A High Quality Draft Consensus Sequence of the Genome of a Heterozygous Grapevine Variety

PubMed Central

Cartwright, Dustin A.; Cestaro, Alessandro; Pruss, Dmitry; Pindo, Massimo; FitzGerald, Lisa M.; Vezzulli, Silvia; Reid, Julia; Malacarne, Giulia; Iliev, Diana; Coppola, Giuseppina; Wardell, Bryan; Micheletti, Diego; Macalma, Teresita; Facci, Marco; Mitchell, Jeff T.; Perazzolli, Michele; Eldredge, Glenn; Gatto, Pamela; Oyzerski, Rozan; Moretto, Marco; Gutin, Natalia; Stefanini, Marco; Chen, Yang; Segala, Cinzia; Davenport, Christine; Demattè, Lorenzo; Mraz, Amy; Battilana, Juri; Stormo, Keith; Costa, Fabrizio; Tao, Quanzhou; Si-Ammour, Azeddine; Harkins, Tim; Lackey, Angie; Perbost, Clotilde; Taillon, Bruce; Stella, Alessandra; Solovyev, Victor; Fawcett, Jeffrey A.; Sterck, Lieven; Vandepoele, Klaas; Grando, Stella M.; Toppo, Stefano; Moser, Claudio; Lanchbury, Jerry; Bogden, Robert; Skolnick, Mark; Sgaramella, Vittorio; Bhatnagar, Satish K.; Fontana, Paolo; Gutin, Alexander; Van de Peer, Yves; Salamini, Francesco; Viola, Roberto

2007-01-01

Background Worldwide, grapes and their derived products have a large market. The cultivated grape species Vitis vinifera has potential to become a model for fruit trees genetics. Like many plant species, it is highly heterozygous, which is an additional challenge to modern whole genome shotgun sequencing. In this paper a high quality draft genome sequence of a cultivated clone of V. vinifera Pinot Noir is presented. Principal Findings We estimate the genome size of V. vinifera to be 504.6 Mb. Genomic sequences corresponding to 477.1 Mb were assembled in 2,093 metacontigs and 435.1 Mb were anchored to the 19 linkage groups (LGs). The number of predicted genes is 29,585, of which 96.1% were assigned to LGs. This assembly of the grape genome provides candidate genes implicated in traits relevant to grapevine cultivation, such as those influencing wine quality, via secondary metabolites, and those connected with the extreme susceptibility of grape to pathogens. Single nucleotide polymorphism (SNP) distribution was consistent with a diffuse haplotype structure across the genome. Of around 2,000,000 SNPs, 1,751,176 were mapped to chromosomes and one or more of them were identified in 86.7% of anchored genes. The relative age of grape duplicated genes was estimated and this made possible to reveal a relatively recent Vitis-specific large scale duplication event concerning at least 10 chromosomes (duplication not reported before). Conclusions Sanger shotgun sequencing and highly efficient sequencing by synthesis (SBS), together with dedicated assembly programs, resolved a complex heterozygous genome. A consensus sequence of the genome and a set of mapped marker loci were generated. Homologous chromosomes of Pinot Noir differ by 11.2% of their DNA (hemizygous DNA plus chromosomal gaps). SNP markers are offered as a tool with the potential of introducing a new era in the molecular breeding of grape. PMID:18094749

Influence of fiber quality on draftometer measurements

USDA-ARS?s Scientific Manuscript database

Fiber-to-fiber and fiber-to-machine friction play an important role in determining textile processing efficiency and end-product quality. A process, known as drafting, is used to control the attenuation of the fiber mass being processed in carding, drawing and spinning. The amount of attenuation t...

Effects of drafting on energy expenditure in in-line skating.

PubMed

Millet, G P; Geslan, R; Ferrier, R; Candau, R; Varray, A

2003-09-01

The purpose of this study was to measure the effects of drafting on energy expenditure in in-line skating, and to investigate whether the "benefit of drafting", i.e. the decrease in energy expenditure, was different between two velocities and two distances separating the two skaters. Eight recreational in-line skaters performed six exercises of 6 min, at 2 velocities (V(1) = 5.51+/-0.45 m x sec(-1); V(2) = 7.01+/-0.67 m x sec(-1)) in 3 conditions (ND = without drafting; D(1)=0.74-0.87 m "close"; D(2) = 1.19-1.36 m "far"). Collection of expired gas was carried out using a breath-by-breath portable gas analyser K4(b2) and the distance between the skaters was measured by video analysis. The skaters' energy expenditure was reduced in all drafting conditions; between D(1) and ND by 9.6+/-4.4% at V(1) and by 2.7+/-3.3% at V(2); between D(2) and ND by 8.8+/-6.0% at V(1) and by 4.2+/-4.8% at V(2). This reduction was significantly (p<0.05) more important at V(1) than V(2) and no differences were observed between D(1) and D(2). In in-line skating, the technical difficulties for drafting efficiently, especially while cornering, resulted in a reduced "benefit of drafting" at high velocity than in other sports. Moreover, the need for the subject to adjust their own cycle frequency to that of the lead skater while drafting "close" would explain partly that there were no significant differences between drafting at D(1) and D(2). These results suggest that the drafting technique should be emphasized in training, especially in non-skilled skaters at high velocities and when cornering.

77 FR 38789 - Notice of Availability of Draft Waste Incidental to Reprocessing Evaluation for the Concentrator...

Federal Register 2010, 2011, 2012, 2013, 2014

2012-06-29

... DEPARTMENT OF ENERGY Notice of Availability of Draft Waste Incidental to Reprocessing Evaluation...: Notice of availability. SUMMARY: The Department of Energy (DOE) announces the availability of a draft... Center in West Valley, New York, are waste incidental to reprocessing and thus are not high-level...

A Menu of Options for Developing Tribal Air Grant Work Plans and Managing Grants for Environmental Results

EPA Pesticide Factsheets

Tool to assist tribes that apply for Clean Air Act (CAA) funding to draft more effective work plans for projects that will develop tribal knowledge of air quality issues and build tribal expertise to manage air quality on tribal lands.

AIR QUALITY CRITERIA FOR PARTICULATE MATTER, VOLUMES I-III, (EXTERNAL REVIEW DRAFT, 1995)

EPA Science Inventory

There is no abstract available for these documents.

If further information is requested, please refer to the bibliographic citation and contact the Technical Information Staff at the number listed above.

• Air Quality Criteria for Particulate Matter, Volume I, Extern...

• 77 FR 12818 - Intent To Prepare a Draft Supplemental Environmental Impact Statement for the Proposed Rio Grande...

  Federal Register 2010, 2011, 2012, 2013, 2014

  2012-03-02

  ..., floodplain development, water quality, ecological resources, endangered species, wildlife refuge objectives, social welfare, human safety, cultural resources, and aesthetic qualities. Development and implementation... risk management study along the Rio Grande from San Acacia downstream to San Marcial in Socorro County...

• VALIDATION OF AMBIENT WATER QUALITY CRITERIA (AWQC) BIOACCUMULATION METHODOLOGY USING FIELD DATA FROM GREEN BAY AND THE HUDSON RIVER

  EPA Science Inventory

  In 1998, EPA published its draft revision to the methodology for deriving ambient water quality criteria to protect human health. Four methods were proposed to determine lipid-normalized bioaccumulation factors based on freely-dissolved water concentrations (BAFs) for nonpolar or...

• Genome analysis of Diploscapter coronatus: insights into molecular peculiarities of a nematode with parthenogenetic reproduction.

  PubMed

  Hiraki, Hideaki; Kagoshima, Hiroshi; Kraus, Christopher; Schiffer, Philipp H; Ueta, Yumiko; Kroiher, Michael; Schierenberg, Einhard; Kohara, Yuji

  2017-06-24

  Sexual reproduction involving the fusion of egg and sperm is prevailing among eukaryotes. In contrast, the nematode Diploscapter coronatus, a close relative of the model Caenorhabditis elegans, reproduces parthenogenetically. Neither males nor sperm have been observed and some steps of meiosis are apparently skipped in this species. To uncover the genomic changes associated with the evolution of parthenogenesis in this nematode, we carried out a genome analysis. We obtained a 170 Mbp draft genome in only 511 scaffolds with a N 50 length of 1 Mbp. Nearly 90% of these scaffolds constitute homologous pairs with a 5.7% heterozygosity on average and inversions and translocations, meaning that the 170 Mbp sequences correspond to the diploid genome. Fluorescent staining shows that the D. coronatus genome consists of two chromosomes (2n = 2). In our genome annotation, we found orthologs of 59% of the C. elegans genes. However, a number of genes were missing or very divergent. These include genes involved in sex determination (e.g. xol-1, tra-2) and meiosis (e.g. the kleisins rec-8 and coh-3/4) giving a possible explanation for the absence of males and the second meiotic division. The high degree of heterozygosity allowed us to analyze the expression level of individual alleles. Most of the homologous pairs show very similar expression levels but others exhibit a 2-5-fold difference. Our high-quality draft genome of D. coronatus reveals the peculiarities of the genome of parthenogenesis and provides some clues to the genetic basis for parthenogenetic reproduction. This draft genome should be the basis to elucidate fundamental questions related to parthenogenesis such as its origin and mechanisms through comparative analyses with other nematodes. Furthermore, being the closest outgroup to the genus Caenorhabditis, the draft genome will help to disclose many idiosyncrasies of the model C. elegans and its congeners in future studies.

• Draft genome of the protandrous Chinese black porgy, Acanthopagrus schlegelii.

  PubMed

  Zhang, Zhiyong; Zhang, Kai; Chen, Shuyin; Zhang, Zhiwei; Zhang, Jinyong; You, Xinxin; Bian, Chao; Xu, Jin; Jia, Chaofeng; Qiang, Jun; Zhu, Fei; Li, Hongxia; Liu, Hailin; Shen, Dehua; Ren, Zhonghong; Chen, Jieming; Li, Jia; Gao, Tianheng; Gu, Ruobo; Xu, Junmin; Shi, Qiong; Xu, Pao

  2018-04-01

  As one of the most popular and valuable commercial marine fishes in China and East Asian countries, the Chinese black porgy (Acanthopagrus schlegelii), also known as the blackhead seabream, has some attractive characteristics such as fast growth rate, good meat quality, resistance to diseases, and excellent adaptability to various environments. Furthermore, the black porgy is a good model for investigating sex changes in fish due to its protandrous hermaphroditism. Here, we obtained a high-quality genome assembly of this interesting teleost species and performed a genomic survey on potential genes associated with the sex-change phenomenon. We generated 175.4 gigabases (Gb) of clean sequence reads using a whole-genome shotgun sequencing strategy. The final genome assembly is approximately 688.1 megabases (Mb), accounting for 93% of the estimated genome size (739.6 Mb). The achieved scaffold N50 is 7.6 Mb, reaching a relatively high level among sequenced fish species. We identified 19 465 protein-coding genes, which had an average transcript length of 17.3 kb. By performing a comparative genomic analysis, we found 3 types of genes potentially associated with sex change, which are useful for studying the genetic basis of the protandrous hermaphroditism. We provide a draft genome assembly of the Chinese black porgy and discuss the potential genetic mechanisms of sex change. These data are also an important resource for studying the biology and for facilitating breeding of this economically important fish.

• The Genomics Education Partnership: Successful Integration of Research into Laboratory Classes at a Diverse Group of Undergraduate Institutions

  PubMed Central

  Shaffer, Christopher D.; Alvarez, Consuelo; Bailey, Cheryl; Barnard, Daron; Bhalla, Satish; Chandrasekaran, Chitra; Chandrasekaran, Vidya; Chung, Hui-Min; Dorer, Douglas R.; Du, Chunguang; Eckdahl, Todd T.; Poet, Jeff L.; Frohlich, Donald; Goodman, Anya L.; Gosser, Yuying; Hauser, Charles; Hoopes, Laura L.M.; Johnson, Diana; Jones, Christopher J.; Kaehler, Marian; Kokan, Nighat; Kopp, Olga R.; Kuleck, Gary A.; McNeil, Gerard; Moss, Robert; Myka, Jennifer L.; Nagengast, Alexis; Morris, Robert; Overvoorde, Paul J.; Shoop, Elizabeth; Parrish, Susan; Reed, Kelynne; Regisford, E. Gloria; Revie, Dennis; Rosenwald, Anne G.; Saville, Ken; Schroeder, Stephanie; Shaw, Mary; Skuse, Gary; Smith, Christopher; Smith, Mary; Spana, Eric P.; Spratt, Mary; Stamm, Joyce; Thompson, Jeff S.; Wawersik, Matthew; Wilson, Barbara A.; Youngblom, Jim; Leung, Wilson; Buhler, Jeremy; Mardis, Elaine R.; Lopatto, David

  2010-01-01

  Genomics is not only essential for students to understand biology but also provides unprecedented opportunities for undergraduate research. The goal of the Genomics Education Partnership (GEP), a collaboration between a growing number of colleges and universities around the country and the Department of Biology and Genome Center of Washington University in St. Louis, is to provide such research opportunities. Using a versatile curriculum that has been adapted to many different class settings, GEP undergraduates undertake projects to bring draft-quality genomic sequence up to high quality and/or participate in the annotation of these sequences. GEP undergraduates have improved more than 2 million bases of draft genomic sequence from several species of Drosophila and have produced hundreds of gene models using evidence-based manual annotation. Students appreciate their ability to make a contribution to ongoing research, and report increased independence and a more active learning approach after participation in GEP projects. They show knowledge gains on pre- and postcourse quizzes about genes and genomes and in bioinformatic analysis. Participating faculty also report professional gains, increased access to genomics-related technology, and an overall positive experience. We have found that using a genomics research project as the core of a laboratory course is rewarding for both faculty and students. PMID:20194808

• Two low coverage bird genomes and a comparison of reference-guided versus de novo genome assemblies.

  PubMed

  Card, Daren C; Schield, Drew R; Reyes-Velasco, Jacobo; Fujita, Matthew K; Andrew, Audra L; Oyler-McCance, Sara J; Fike, Jennifer A; Tomback, Diana F; Ruggiero, Robert P; Castoe, Todd A

  2014-01-01

  As a greater number and diversity of high-quality vertebrate reference genomes become available, it is increasingly feasible to use these references to guide new draft assemblies for related species. Reference-guided assembly approaches may substantially increase the contiguity and completeness of a new genome using only low levels of genome coverage that might otherwise be insufficient for de novo genome assembly. We used low-coverage (∼3.5-5.5x) Illumina paired-end sequencing to assemble draft genomes of two bird species (the Gunnison Sage-Grouse, Centrocercus minimus, and the Clark's Nutcracker, Nucifraga columbiana). We used these data to estimate de novo genome assemblies and reference-guided assemblies, and compared the information content and completeness of these assemblies by comparing CEGMA gene set representation, repeat element content, simple sequence repeat content, and GC isochore structure among assemblies. Our results demonstrate that even lower-coverage genome sequencing projects are capable of producing informative and useful genomic resources, particularly through the use of reference-guided assemblies.

• Two low coverage bird genomes and a comparison of reference-guided versus de novo genome assemblies

  USGS Publications Warehouse

  Card, Daren C.; Schield, Drew R.; Reyes-Velasco, Jacobo; Fujita, Matthre K.; Andrew, Audra L.; Oyler-McCance, Sara J.; Fike, Jennifer A.; Tomback, Diana F.; Ruggiero, Robert P.; Castoe, Todd A.

  2014-01-01

  As a greater number and diversity of high-quality vertebrate reference genomes become available, it is increasingly feasible to use these references to guide new draft assemblies for related species. Reference-guided assembly approaches may substantially increase the contiguity and completeness of a new genome using only low levels of genome coverage that might otherwise be insufficient for de novo genome assembly. We used low-coverage (~3.5–5.5x) Illumina paired-end sequencing to assemble draft genomes of two bird species (the Gunnison Sage-Grouse, Centrocercus minimus, and the Clark's Nutcracker, Nucifraga columbiana). We used these data to estimate de novo genome assemblies and reference-guided assemblies, and compared the information content and completeness of these assemblies by comparing CEGMA gene set representation, repeat element content, simple sequence repeat content, and GC isochore structure among assemblies. Our results demonstrate that even lower-coverage genome sequencing projects are capable of producing informative and useful genomic resources, particularly through the use of reference-guided assemblies.

• High quality draft genome sequence of Janthinobacterium psychrotolerans sp. nov., isolated from a frozen freshwater pond.

  PubMed

  Gong, Xianzhe; Skrivergaard, Stig; Korsgaard, Benjamin Smed; Schreiber, Lars; Marshall, Ian P G; Finster, Kai; Schramm, Andreas

  2017-01-01

  Strain S3-2 T , isolated from sediment of a frozen freshwater pond, shares 99% 16S rRNA gene sequence identity with strains of the genus Janthinobacterium . Strain S3-2 T is a facultative anaerobe that lacks the ability to produce violacein but shows antibiotic resistance, psychrotolerance, incomplete denitrification, and fermentation. The draft genome of strain S3-2 T has a size of ~5.8 Mbp and contains 5,297 genes, including 115 RNA genes. Based on the phenotypic properties of the strain, the low in silico DNA-DNA hybridization (DDH) values with related genomes (<35%), and the low whole genome-based average nucleotide identity (ANI) (<86%) with other strains within the genus Janthinobacterium, we propose that strain S3-2 T is the type strain (= DSM 102223 = LMG 29653) of a new species within this genus. We propose the name Janthinobacterium psychrotolerans sp. nov. to emphasize the capability of the strain to grow at low temperatures.

• Permanent draft genome sequence of the gliding predator Saprospira grandis strain Sa g1 (= HR1)

  DOE Office of Scientific and Technical Information (OSTI.GOV)

  Mavromatis, K; Chertkov, Olga; Lapidus, Alla L.

  2012-01-01

  Saprospira grandis Gross et al. 1911 is a member of the Saprospiraceae, a family in the class 'Sphingobacteria' that remains poorly characterized at the genomic level. The species is known for preying on other marine bacteria via 'ixotrophy'. S. grandis strain Sa g1 was isolated from decaying crab carapace in France and was selected for genome sequencing because of its isolated location in the tree of life. Only one type strain genome has been published so far from the Saprospiraceae, while the sequence of strain Sa g1 represents the second genome to be published from a non-type strain of S.more » grandis. Here we describe the features of this organism, together with the complete genome sequence and annotation. The 4,495,250 bp long Improved-High-Quality draft of the genome with its 3,536 protein-coding and 62 RNA genes is a part of the Genomic Encyclopedia of Bacteria and Archaea project.« less

• 75 FR 54694 - Notice of Availability of a Supplemental Draft Environmental Impact Statement for the...

  Federal Register 2010, 2011, 2012, 2013, 2014

  2010-09-08

  ... Supplemental Draft Environmental Impact Statement for the DesertXpress High-Speed Passenger Rail Project AGENCY... for the DesertXpress High- Speed Passenger Train Project (Project). FRA is the lead agency for the... involve the construction and operation of an interstate high-speed passenger train system between...

• 77 FR 43902 - Notice of Availability of a Supplemental Draft Environmental Impact Statement for the California...

  Federal Register 2010, 2011, 2012, 2013, 2014

  2012-07-26

  ... Supplemental Draft Environmental Impact Statement for the California High-Speed Train Project Fresno to... of the California High- Speed Train (HST) Project (Project). FRA is the lead Federal agency and the California High-Speed Rail Authority (Authority) is the lead state agency for the environmental review...

• A physical map of the bovine genome

  PubMed Central

  Snelling, Warren M; Chiu, Readman; Schein, Jacqueline E; Hobbs, Matthew; Abbey, Colette A; Adelson, David L; Aerts, Jan; Bennett, Gary L; Bosdet, Ian E; Boussaha, Mekki; Brauning, Rudiger; Caetano, Alexandre R; Costa, Marcos M; Crawford, Allan M; Dalrymple, Brian P; Eggen, André; Everts-van der Wind, Annelie; Floriot, Sandrine; Gautier, Mathieu; Gill, Clare A; Green, Ronnie D; Holt, Robert; Jann, Oliver; Jones, Steven JM; Kappes, Steven M; Keele, John W; de Jong, Pieter J; Larkin, Denis M; Lewin, Harris A; McEwan, John C; McKay, Stephanie; Marra, Marco A; Mathewson, Carrie A; Matukumalli, Lakshmi K; Moore, Stephen S; Murdoch, Brenda; Nicholas, Frank W; Osoegawa, Kazutoyo; Roy, Alice; Salih, Hanni; Schibler, Laurent; Schnabel, Robert D; Silveri, Licia; Skow, Loren C; Smith, Timothy PL; Sonstegard, Tad S; Taylor, Jeremy F; Tellam, Ross; Van Tassell, Curtis P; Williams, John L; Womack, James E; Wye, Natasja H; Yang, George; Zhao, Shaying

  2007-01-01

  Background Cattle are important agriculturally and relevant as a model organism. Previously described genetic and radiation hybrid (RH) maps of the bovine genome have been used to identify genomic regions and genes affecting specific traits. Application of these maps to identify influential genetic polymorphisms will be enhanced by integration with each other and with bacterial artificial chromosome (BAC) libraries. The BAC libraries and clone maps are essential for the hybrid clone-by-clone/whole-genome shotgun sequencing approach taken by the bovine genome sequencing project. Results A bovine BAC map was constructed with HindIII restriction digest fragments of 290,797 BAC clones from animals of three different breeds. Comparative mapping of 422,522 BAC end sequences assisted with BAC map ordering and assembly. Genotypes and pedigree from two genetic maps and marker scores from three whole-genome RH panels were consolidated on a 17,254-marker composite map. Sequence similarity allowed integrating the BAC and composite maps with the bovine draft assembly (Btau3.1), establishing a comprehensive resource describing the bovine genome. Agreement between the marker and BAC maps and the draft assembly is high, although discrepancies exist. The composite and BAC maps are more similar than either is to the draft assembly. Conclusion Further refinement of the maps and greater integration into the genome assembly process may contribute to a high quality assembly. The maps provide resources to associate phenotypic variation with underlying genomic variation, and are crucial resources for understanding the biology underpinning this important ruminant species so closely associated with humans. PMID:17697342

• Draft Genome Sequence of Komagataeibacter rhaeticus Strain AF1, a High Producer of Cellulose, Isolated from Kombucha Tea.

  PubMed

  Dos Santos, Renato Augusto Corrêa; Berretta, Andresa A; Barud, Hernane da Silva; Ribeiro, Sidney José Lima; González-García, Laura Natalia; Zucchi, Tiago Domingues; Goldman, Gustavo H; Riaño-Pachón, Diego M

  2014-07-24

  Here, we present the draft genome sequence of Komagatabaeicter rhaeticus strain AF1, which was isolated from Kombucha tea and is capable of producing high levels of cellulose. Copyright © 2014 dos Santos et al.

• 15 CFR 996.31 - Termination of the Quality Assurance Program.

  Code of Federal Regulations, 2010 CFR

  2010-01-01

  ... 15 Commerce and Foreign Trade 3 2010-01-01 2010-01-01 false Termination of the Quality Assurance Program. 996.31 Section 996.31 Commerce and Foreign Trade Regulations Relating to Commerce and Foreign... inability of the standards-drafting group to reach a consensus on the content of the standard; (2) Valid...

76 FR 63909 - Draft Programmatic Environmental Impact Statement (EIS) for Modernization of Training...

Federal Register 2010, 2011, 2012, 2013, 2014

2011-10-14

... the training requirements of military units in Hawai`i. This modernization would improve the quality... at PTA are old, are operating beyond their useful life and do not meet current DoD design standards... with the possible effects to air quality, historic resources, and threatened and endangered species...

76 FR 5386 - Draft Guidance for Industry: Pre-Storage Leukocyte Reduction of Whole Blood and Blood Components...

Federal Register 2010, 2011, 2012, 2013, 2014

2011-01-31

... intended for transfusion, including recommendations for validation and quality control monitoring of the..., including recommendations for validation and quality control monitoring of the leukocyte reduction process... control number 0910-0052; the collections of information in 21 CFR 606.100(b), 606.100(c), and 606.121...

78 FR 44932 - Notice of Intent To Prepare a Draft Environmental Impact Statement for the Pearl River Section...

Federal Register 2010, 2011, 2012, 2013, 2014

2013-07-25

...: Notice of Intent. SUMMARY: Pursuant to Section 211 of the Water Resources Development Act of 1996, the... Water Resources Development Act of 1996, to investigate measures to alleviate flooding in the study area... commercial fisheries, wildlife resources, water quality, air quality, threatened or endangered species...

75 FR 52549 - Environmental Impact Statement; Alabama Beach Mouse Draft General Conservation Plan; Fort Morgan...

Federal Register 2010, 2011, 2012, 2013, 2014

2010-08-26

... the ABM GCP is established, property owners who wish to develop low-density residences on the Fort... potentially significant impacts on biological resources, land use, air quality, water quality, water resources... future development could occur within these areas. It is important that suitable habitat be maintained...

The Contribution of Quality Assurance Reviews to Development in School Systems. Draft.

ERIC Educational Resources Information Center

Cuttance, Peter

This paper discusses key aspects of the British experience with school review and evaluation, which influenced the development of the South Australian framework for school evaluation. The discussion combines the requirements of accountability and development within a framework of quality assurance. The paper describes shortcomings of the internal…

Evidence-informed recommendations for rehabilitation with older adults living with HIV: a knowledge synthesis

PubMed Central

O'Brien, Kelly K; Solomon, Patricia; Trentham, Barry; MacLachlan, Duncan; MacDermid, Joy; Tynan, Anne-Marie; Baxter, Larry; Casey, Alan; Chegwidden, William; Robinson, Greg; Tran, Todd; Wu, Janet; Zack, Elisse

2014-01-01

Objective Our aim was to develop evidence-informed recommendations for rehabilitation with older adults living with HIV. Design We conducted a knowledge synthesis, combining research evidence specific to HIV, rehabilitation and ageing, with evidence on rehabilitation interventions for common comorbidities experienced by older adults with HIV. Methods We included highly relevant HIV-specific research addressing rehabilitation and ageing (stream A) and high-quality evidence on the effectiveness of rehabilitation interventions for common comorbidities experienced by older adults ageing with HIV (stream B). We extracted and synthesised relevant data from the evidence to draft evidence-informed recommendations for rehabilitation. Draft recommendations were refined based on people living with HIV (PLHIV) and clinician experience, values and preferences, reviewed by an interprofessional team for Grading of Recommendations Assessment, Development, and Evaluation (GRADE) (quality) rating and revision and then circulated to PLHIV and clinicians for external endorsement and final refinement. We then devised overarching recommendations to broadly guide rehabilitation with older adults living with HIV. Results This synthesis yielded 8 overarching and 52 specific recommendations. Thirty-six specific recommendations were derived from 108 moderate-level or high-level research articles (meta-analyses and systematic reviews) that described the effectiveness of rehabilitation interventions for comorbidities that may be experienced by older adults with HIV. Recommendations addressed rehabilitation interventions across eight health conditions: bone and joint disorders, cancer, stroke, cardiovascular disease, mental health challenges, cognitive impairments, chronic obstructive pulmonary disease and diabetes. Sixteen specific recommendations were derived from 42 research articles specific to rehabilitation with older adults with HIV. The quality of evidence from which these recommendations were derived was either low or very low, consisting primarily of narrative reviews or descriptive studies with small sample sizes. Recommendations addressed approaches to rehabilitation assessment and interventions, and contextual factors to consider for rehabilitation with older adults living with HIV. Conclusions These evidence-informed recommendations provide a guide for rehabilitation with older adults living with HIV. PMID:24833687

Integrated Science Assessment (ISA) for Sulfur Oxides ...

EPA Pesticide Factsheets

EPA has announced that the Second External Review Draft of the Integrated Science Assessment (ISA) for Sulfur Oxides – Health Criteria has been made available for independent peer review and public review. This draft ISA document represents a concise synthesis and evaluation of the most policy-relevant science and will ultimately provide the scientific bases for EPA’s decision regarding whether the current standard for SO2 sufficiently protects public health. Sulfur oxides is one of six principal (or “criteria”) pollutants for which EPA has established national ambient air quality standards (NAAQS).

Integrated Science Assessment (ISA) for Sulfur Oxides ...

EPA Pesticide Factsheets

EPA has announced that the First External Review Draft of the Integrated Science Assessment (ISA) for Sulfur Oxides – Health Criteria has been made available for independent peer review and public review. This draft ISA document represents a concise synthesis and evaluation of the most policy-relevant science and will ultimately provide the scientific bases for EPA’s decision regarding whether the current standard for SO2 sufficiently protects public health. Sulfur oxides is one of six principal (or “criteria”) pollutants for which EPA has established national ambient air quality standards (NAAQS).

High-Resolution 3-T Magnetic Resonance Imaging of the Shoulder in Nonsymptomatic Professional Baseball Pitcher Draft Picks.

PubMed

Del Grande, Filippo; Aro, Michael; Jalali Farahani, Sahar; Cosgarea, Andrew; Wilckens, John; Carrino, John A

2016-01-01

To retrospectively assess the qualitative and quantitative high-resolution 3-T magnetic resonance imaging (MRI) findings in nonsymptomatic baseball pitcher draft picks. Institutional review board-approved and HIPAA compliant study. Three-Tesla MRI of the dominant shoulder of 19 asymptomatic baseball pitcher draft picks and detailed clinical examination was performed before contract signing. Two radiologists performed independently qualitative and quantitative evaluation of shoulder structures. Descriptive statistics were performed. Sixty-eight percent (13/19), 32% (6/19), and 21% (4/19) of the baseball pitcher draft picks showed tendinopathy, partial thickness tendon tear of the supraspinatus, and acromioclavicular joint osteoarthritis, respectively. Glenohumeral subluxation, glenoid remodeling, and Bennett lesion were present in 53% (10/19), 79% (15/19), and 21% (4/19) of the subjects, respectively. Interclass coefficient was 0.633 to 0.863 and κ was 0.27 to 1. In asymptomatic baseball pitcher draft picks, 3-T MRI frequently shows abnormalities involving rotator cuff tendons, the coracohumeral, inferior glenohumeral, labrum, and osseous structures.

Draft Genome Sequence of Solibacillus kalamii, Isolated from an Air Filter Aboard the International Space Station.

PubMed

Seuylemezian, Arman; Singh, Nitin K; Vaishampayan, Parag; Venkateswaran, Kasthuri

2017-08-31

We report here the draft genome of Solibacillus kalamii ISSFR-015, isolated from a high-energy particulate arrestance filter aboard the International Space Station. The draft genome sequence of this strain contains 3,809,180 bp with an estimated G+C content of 38.61%. Copyright © 2017 Seuylemezian et al.

Draft genome sequence of the silver pomfret fish, Pampus argenteus.

PubMed

AlMomin, Sabah; Kumar, Vinod; Al-Amad, Sami; Al-Hussaini, Mohsen; Dashti, Talal; Al-Enezi, Khaznah; Akbar, Abrar

2016-01-01

Silver pomfret, Pampus argenteus, is a fish species from coastal waters. Despite its high commercial value, this edible fish has not been sequenced. Hence, its genetic and genomic studies have been limited. We report the first draft genome sequence of the silver pomfret obtained using a Next Generation Sequencing (NGS) technology. We assembled 38.7 Gb of nucleotides into scaffolds of 350 Mb with N50 of about 1.5 kb, using high quality paired end reads. These scaffolds represent 63.7% of the estimated silver pomfret genome length. The newly sequenced and assembled genome has 11.06% repetitive DNA regions, and this percentage is comparable to that of the tilapia genome. The genome analysis predicted 16 322 genes. About 91% of these genes showed homology with known proteins. Many gene clusters were annotated to protein and fatty-acid metabolism pathways that may be important in the context of the meat texture and immune system developmental processes. The reference genome can pave the way for the identification of many other genomic features that could improve breeding and population-management strategies, and it can also help characterize the genetic diversity of P. argenteus.

77 FR 70142 - Initialed Draft Revision to the Agreement Suspending the Antidumping Investigation on Certain Hot...

Federal Register 2010, 2011, 2012, 2013, 2014

2012-11-23

...; API 5L Grades A & B....... Two--HSLA & HSLA-F Quality Grades: A572, A1011-HSLAS; A1018-HSLAS, A1011-HSLAS- $661.92 F; A1018-HSLAS-F; API 5L Gr. X42, X46, X52, X56, X60; API 5CT Grades J55 and K55 Three--High Grade Coils and Sheets for Pipes and Casings API 5L Gr. X65, X70, and X80 $770.24 Section III.E is...

Environmental Impact Analysis Process. Volume 1. Preliminary Draft Environmental Impact Statement Construction and Operation of Space Launch Complex 7, Vandenberg Air Force Base, California

DTIC Science & Technology

1989-04-06

Cfiada Agua Viva is a south-flowing, perennial drainage located east of the project area and fed by two springs near Wild Horse Flats. Perennial yields...from this drainage are expected to be less than five gallons per minute (gpm), or 60 acre-feet per year. Caflada Agua Viva has a watershed area of... Agua Viva drainages are shown in Table 3.2.1 (Surface Water Quality, Point Arguello Area). Notably high values of total hardness, specific

[SCHWABE Company's patent portfolio of Ginkgo biloba preparation].

PubMed

Liu, Wei; Cheng, Xin-Min; Geng, Dong-Mei; Tan, Wei; Zou, Wen-Jun

2014-09-01

SCHWABE Company in German is the first and largest manufacturer of Ginkgo biloba preparation. The company not only has leading technology in this field, but also protects its own market effectively through the high quality of patent drafting and exactly patent layout. Based on multi-angle analysis for patent portfolio of G. biloba preparation at application time, legal status, globally layout, Chinese layout, the article provides technical reference of research and development of G. biloba, also provides valuable experience of traditonal Chinese medicine patent portfolio layout for Chinese enterprises.

Single-molecule sequencing of the desiccation-tolerant grass Oropetium thomaeum

DOE Office of Scientific and Technical Information (OSTI.GOV)

VanBuren, Robert; Bryant, Doug; Edger, Patrick P.

Plant genomes, and eukaryotic genomes in general, are typically repetitive, polyploid and heterozygous, which complicates genome assembly1. The short read lengths of early Sanger and current next-generation sequencing platforms hinder assembly through complex repeat regions, and many draft and reference genomes are fragmented, lacking skewed GC and repetitive intergenic sequences, which are gaining importance due to projects like the Encyclopedia of DNA Elements (ENCODE). Here we report the whole-genome sequencing and assembly of the desiccation-tolerant grass Oropetium thomaeum. Using only single-molecule real-time sequencing, which generates long (>16 kilobases) reads with random errors, we assembled 99% (244 megabases) of the Oropetiummore » genome into 625 contigs with an N50 length of 2.4 megabases. Oropetium is an example of a ‘near-complete’ draft genome which includes gapless coverage over gene space as well as intergenic sequences such as centromeres, telomeres, transposable elements and rRNA clusters that are typically unassembled in draft genomes. Oropetium has 28,466 protein-coding genes and 43% repeat sequences, yet with 30% more compact euchromatic regions it is the smallest known grass genome. As a result, the Oropetium genome demonstrates the utility of single-molecule real-time sequencing for assembling high-quality plant and other eukaryotic genomes, and serves as a valuable resource for the plant comparative genomics community.« less

Single-molecule sequencing of the desiccation-tolerant grass Oropetium thomaeum

DOE PAGES

VanBuren, Robert; Bryant, Doug; Edger, Patrick P.; ...

2015-11-11

Plant genomes, and eukaryotic genomes in general, are typically repetitive, polyploid and heterozygous, which complicates genome assembly1. The short read lengths of early Sanger and current next-generation sequencing platforms hinder assembly through complex repeat regions, and many draft and reference genomes are fragmented, lacking skewed GC and repetitive intergenic sequences, which are gaining importance due to projects like the Encyclopedia of DNA Elements (ENCODE). Here we report the whole-genome sequencing and assembly of the desiccation-tolerant grass Oropetium thomaeum. Using only single-molecule real-time sequencing, which generates long (>16 kilobases) reads with random errors, we assembled 99% (244 megabases) of the Oropetiummore » genome into 625 contigs with an N50 length of 2.4 megabases. Oropetium is an example of a ‘near-complete’ draft genome which includes gapless coverage over gene space as well as intergenic sequences such as centromeres, telomeres, transposable elements and rRNA clusters that are typically unassembled in draft genomes. Oropetium has 28,466 protein-coding genes and 43% repeat sequences, yet with 30% more compact euchromatic regions it is the smallest known grass genome. As a result, the Oropetium genome demonstrates the utility of single-molecule real-time sequencing for assembling high-quality plant and other eukaryotic genomes, and serves as a valuable resource for the plant comparative genomics community.« less

Mission-oriented requirements for updating MIL-H-8501: Calspan proposed structure and rationale

NASA Technical Reports Server (NTRS)

Chalk, C. R.; Radford, R. C.

1985-01-01

This report documents the effort by Arvin/Calspan Corporation to formulate a revision of MIL-H-8501A in terms of Mission-Oriented Flying Qualities Requirements for Military Rotorcraft. Emphasis is placed on development of a specification structure which will permit addressing Operational Missions and Flight Phases, Flight Regions, Classification of Required Operational Capability, Categorization of Flight Phases, and Levels of Flying Qualities. A number of definitions is established to permit addressing the rotorcraft state, flight envelopes, environments, and the conditions under which degraded flying qualities are permitted. Tentative requirements are drafted for Required Operational Capability Class 1. Also included is a Background Information and Users Guide for the draft specification structure proposed for the MIL-H-8501A revision. The report also contains a discussion of critical data gaps and attempts to prioritize these data gaps and to suggest experiments that should be performed to generate data needed to support formulation of quantitative design criteria for the additional Operational Capability Classes 2, 3, and 4.

Quality scores for 32,000 genomes

DOE PAGES

Land, Miriam L.; Hyatt, Doug; Jun, Se-Ran; ...

2014-12-08

More than 80% of the microbial genomes in GenBank are of ‘draft’ quality (12,553 draft vs. 2,679 finished, as of October, 2013). In this study, we have examined all the microbial DNA sequences available for complete, draft, and Sequence Read Archive genomes in GenBank as well as three other major public databases, and assigned quality scores for more than 30,000 prokaryotic genome sequences. Scores were assigned using four categories: the completeness of the assembly, the presence of full-length rRNA genes, tRNA composition and the presence of a set of 102 conserved genes in prokaryotes. Most (~88%) of the genomes hadmore » quality scores of 0.8 or better and can be safely used for standard comparative genomics analysis. We compared genomes across factors that may influence the score. We found that although sequencing depth coverage of over 100x did not ensure a better score, sequencing read length was a better indicator of sequencing quality. With few exceptions, most of the 30,000 genomes have nearly all the 102 essential genes. The score can be used to set thresholds for screening data when analyzing “all published genomes” and reference data is either not available or not applicable. The scores highlighted organisms for which commonly used tools do not perform well. This information can be used to improve tools and to serve a broad group of users as more diverse organisms are sequenced. Finally and unexpectedly, the comparison of predicted tRNAs across 15,000 high quality genomes showed that anticodons beginning with an ‘A’ (codons ending with a ‘U’) are almost non-existent, with the exception of one arginine codon (CGU); this has been noted previously in the literature for a few genomes, but not with the depth found here.« less

Quality scores for 32,000 genomes

DOE Office of Scientific and Technical Information (OSTI.GOV)

Land, Miriam L.; Hyatt, Doug; Jun, Se-Ran

More than 80% of the microbial genomes in GenBank are of ‘draft’ quality (12,553 draft vs. 2,679 finished, as of October, 2013). In this study, we have examined all the microbial DNA sequences available for complete, draft, and Sequence Read Archive genomes in GenBank as well as three other major public databases, and assigned quality scores for more than 30,000 prokaryotic genome sequences. Scores were assigned using four categories: the completeness of the assembly, the presence of full-length rRNA genes, tRNA composition and the presence of a set of 102 conserved genes in prokaryotes. Most (~88%) of the genomes hadmore » quality scores of 0.8 or better and can be safely used for standard comparative genomics analysis. We compared genomes across factors that may influence the score. We found that although sequencing depth coverage of over 100x did not ensure a better score, sequencing read length was a better indicator of sequencing quality. With few exceptions, most of the 30,000 genomes have nearly all the 102 essential genes. The score can be used to set thresholds for screening data when analyzing “all published genomes” and reference data is either not available or not applicable. The scores highlighted organisms for which commonly used tools do not perform well. This information can be used to improve tools and to serve a broad group of users as more diverse organisms are sequenced. Finally and unexpectedly, the comparison of predicted tRNAs across 15,000 high quality genomes showed that anticodons beginning with an ‘A’ (codons ending with a ‘U’) are almost non-existent, with the exception of one arginine codon (CGU); this has been noted previously in the literature for a few genomes, but not with the depth found here.« less

Draft Genome Sequences of 20 Salmonella enterica subsp. enterica Serovar Typhimurium Strains Isolated from Swine in Santa Catarina, Brazil.

PubMed

Seribelli, Amanda Aparecida; Frazão, Miliane Rodrigues; Gonzales, Júlia Cunha; Cao, Guojie; Leon, Maria Sanchez; Kich, Jalusa Deon; Allard, Marc William; Falcão, Juliana Pfrimer

2018-04-19

Salmonellosis is a disease with a high incidence worldwide, and Salmonella enterica subsp. enterica serovar Typhimurium is one of the most clinically important serovars. We report here the draft genome sequences of 20 S. Typhimurium strains isolated from swine in Santa Catarina, Brazil. These draft genomes will improve our understanding of S. Typhimurium in Brazil.

The impact of peer review on paediatric forensic reports.

PubMed

Kariyawasam, Uditha

2016-10-01

To retrospectively evaluate the common grammar and spelling errors of the medico-legal reports written by the doctors at the Victorian Forensic Paediatric Medical Service (VFPMS) in both Royal Children's Hospital (RCH) and Monash Medical Centre. The reports were evaluated at two points in time; before and after peer review. The aim of the study was to ascertain whether peer review improved the grammar and spelling in VFPMS medico-legal reports. Draft VFPMS reports are sent to the VFPMS medical director for peer review. The current study sampled 50 reports that were sent consecutively to Dr. Anne Smith from 1st of May 2015. The 50 corresponding final reports were then retrieved from the VFPMS database. The 50 pairs of draft and final reports were scored using a 50-point scoring system. The scores of the draft reports were compared to the scores of the final report to assess if there was a change in quality as measured using an explicit criteria audit of report structure, simple grammar, jargon use and spelling. The audit did not include evaluation of the validity of forensic opinions. The overall scores were statistically analysed using descriptive statistics and a paired T-test. The scores of the reports improved by 2.24% when the final reports were compared to the draft reports (p < 0.001). The peer-review process resulted in a significantly higher quality of medico-legal reports. The report writing and peer-review process could be assisted by an abbreviated version of the checklist used for the audit. Crown Copyright © 2016. Published by Elsevier Ltd. All rights reserved.

Opera: reconstructing optimal genomic scaffolds with high-throughput paired-end sequences.

PubMed

Gao, Song; Sung, Wing-Kin; Nagarajan, Niranjan

2011-11-01

Scaffolding, the problem of ordering and orienting contigs, typically using paired-end reads, is a crucial step in the assembly of high-quality draft genomes. Even as sequencing technologies and mate-pair protocols have improved significantly, scaffolding programs still rely on heuristics, with no guarantees on the quality of the solution. In this work, we explored the feasibility of an exact solution for scaffolding and present a first tractable solution for this problem (Opera). We also describe a graph contraction procedure that allows the solution to scale to large scaffolding problems and demonstrate this by scaffolding several large real and synthetic datasets. In comparisons with existing scaffolders, Opera simultaneously produced longer and more accurate scaffolds demonstrating the utility of an exact approach. Opera also incorporates an exact quadratic programming formulation to precisely compute gap sizes (Availability: http://sourceforge.net/projects/operasf/ ).

Opera: Reconstructing Optimal Genomic Scaffolds with High-Throughput Paired-End Sequences

PubMed Central

Gao, Song; Sung, Wing-Kin

2011-01-01

Abstract Scaffolding, the problem of ordering and orienting contigs, typically using paired-end reads, is a crucial step in the assembly of high-quality draft genomes. Even as sequencing technologies and mate-pair protocols have improved significantly, scaffolding programs still rely on heuristics, with no guarantees on the quality of the solution. In this work, we explored the feasibility of an exact solution for scaffolding and present a first tractable solution for this problem (Opera). We also describe a graph contraction procedure that allows the solution to scale to large scaffolding problems and demonstrate this by scaffolding several large real and synthetic datasets. In comparisons with existing scaffolders, Opera simultaneously produced longer and more accurate scaffolds demonstrating the utility of an exact approach. Opera also incorporates an exact quadratic programming formulation to precisely compute gap sizes (Availability: http://sourceforge.net/projects/operasf/). PMID:21929371

78 FR 69365 - Notice of Meeting of the Agricultural Air Quality Task Force

Federal Register 2010, 2011, 2012, 2013, 2014

2013-11-19

... relation to agriculture. Special emphasis will be placed on obtaining a greater understanding about the relationship between agricultural production and air quality. The meeting is open to the public, and a draft... Thursday, December 4-5, 2013, and conclude at 5:00 p.m. each day. A public comment period will be held on...

Air Quality Criteria for Particulate Matter (Fourth External Review Draft) [revised Chapter 9, August 2004

EPA Science Inventory

EPA is in the process of updating and revising, where appropriate, its Air Quality Criteria for Particulate Matter (PM) as issued in 1996 (usually referred to as the Criteria Document). Sections 108 and 109 of the Clean Air Act require that EPA carry out a periodic review and re...

AIR QUALITY CRITERIA FOR PARTICULATE MATTER (FOURTH EXTERNAL REVIEW DRAFT) [REVISED CHAPTERS 7, 8, AND 9, JUNE 2004

EPA Science Inventory

EPA is in the process of updating and revising, where appropriate, its Air Quality Criteria for Particulate Matter as issued in 1996 (usually referred to as the Criteria Document). Sections 108 and 109 of the Clean Air Act require that EPA carry out a periodic review and revisio...

76 FR 23809 - Science Advisory Board Staff Office Notification of a Public Meeting of the Clean Air Scientific...

Federal Register 2010, 2011, 2012, 2013, 2014

2011-04-28

...: Scope and Methods Plan for Welfare Risk and Exposure Assessment (April 2011 Draft). DATES: The CASAC... Ambient Air Quality Standards: Scope and Methods Plan for Health Risk and Exposure Assessment (April 2011), and Ozone National Ambient Air Quality Standards: Scope and Methods Plan for Welfare Risk and Exposure...

77 FR 15087 - Intent To Prepare a Draft Environmental Impact Statement (DEIS) for the Installation of a...

Federal Register 2010, 2011, 2012, 2013, 2014

2012-03-14

... to Section 404 of the Clean Water Act and Section 10 of the Rivers and Harbor Act, from the Village.../Projects/index.html , under the Village of Bald Head Island Terminal Groin Project. SUPPLEMENTARY... DEIS with the North Carolina Division of Water Quality (NCDWQ) to assess the potential water quality...

Race to the Top District Competition: The Data Quality Campaign's Comments on ED's Proposed Criteria

ERIC Educational Resources Information Center

Data Quality Campaign, 2012

2012-01-01

The Data Quality Campaign (DQC) submits these comments in response to the US Department of Education's (ED) draft requirements, priorities, selection criteria, and definitions for the Race to the Top District (RTT-D) competition. DQC applauds ED for including the following components of the proposal: (1) Data is acknowledged throughout the…

75 FR 20826 - Notice of Intent To Prepare a Draft Environmental Impact Statement on Beach and Dune Restoration...

Federal Register 2010, 2011, 2012, 2013, 2014

2010-04-21

... restore the beach and dune by placing approximately 610,000 cubic yards of beach compatible sand along the proposed project's beach. The sand will be hopper-dredged from a relict shoal approximately three to six... analyze the following: aesthetics/visual quality, agricultural resources, air quality, biological...

The Effect of Assessment of Process after Receiving Teacher Feedback

ERIC Educational Resources Information Center

Ruegg, Rachael

2016-01-01

This exploratory study investigated the effect of assessing both process and product compared to assessing written products alone. Two groups of students received teacher feedback over a one-year period. One group was assessed on their revisions in addition to the quality of final drafts, while a second group was assessed on the quality of final…

Patient-reported quality indicators for osteoarthritis: a patient and public generated self-report measure for primary care.

PubMed

Blackburn, Steven; Higginbottom, Adele; Taylor, Robert; Bird, Jo; Østerås, Nina; Hagen, Kåre Birger; Edwards, John J; Jordan, Kelvin P; Jinks, Clare; Dziedzic, Krysia

2016-01-01

People with osteoarthritis desire high quality care, support and information. However, the quality of care for people with OA in general practice is not routinely collected. Quality Indicators can be used to benefit patients by measuring whether minimum standards of quality care are being met from a patient perspective. The aim of this study was to describe how a Research User Group (RUG) worked alongside researchers to co-produce a set of self-reported quality indicators for people with osteoarthritis when visiting their general practitioner or practice nurse (primary care). These were required in the MOSAICS study, which developed and evaluated a new model of supported self-management of OA to implement the NICE quality standards for OA. This article describes the public involvement in the MOSAICS study. This was 1) the co-development by RUG members and researchers of an Osteoarthritis Quality Indicators United Kingdom (OA QI (UK)) questionnaire for use in primary care, and 2) the comparison of the OA QI (UK) with a similar questionnaire developed in Norway. This study shows how important and effective a research user group can be in working with researchers in developing quality care indicators for osteoarthritis for use in a research study and, potentially, routine use in primary care. The questionnaire is intended to benefit patients by enabling the assessment of the quality of primary care for osteoarthritis from a patient's perspective. The OA QI (UK) has been used to examine differences in the quality of osteoarthritis care in four European countries. Background People with osteoarthritis (OA) desire high quality care, support and information about OA. However, the quality of care for people with OA in general practice is not routinely collected. Quality Indicators (QI) can be used to benefit patients by measuring whether minimum standards of quality care (e.g. NICE quality standards) are being met from a patient perspective. A Research User Group (RUG) worked with researchers to co-produce a set of self-report, patient-generated QIs for OA. The QIs were intended for use in the MOSAICS study, which developed and evaluated a new model of supported self-management of OA to implement the NICE guidelines. We report on 1) the co-development of the OA QI (UK) questionnaire for primary care; and 2) the comparison of the content of the OA QI (UK) questionnaire with a parallel questionnaire developed in Norway for the Musculoskeletal Pain in Ullensaker (MUST) study. Methods Researchers were invited to OA RUG meetings. Firstly, RUG members were asked to consider factors important to patients consulting their general practitioner (GP) for OA and then each person rated their five most important. RUG members then discussed these in relation to a systematic review of OA QIs in order to form a list of OA QIs from a patient perspective. RUG members suggested wording and response options for a draft OA QI (UK) questionnaire to assess the QIs. Finally RUG members commented on draft and final versions of the questionnaire and how it compared with a translated Norwegian OA-QI questionnaire. Results RUG members (5 males, 5 females; aged 52-80 years) attended up to four meetings. RUG members ranked 20 factors considered most important to patients consulting their GP for joint pain. Following discussion, a list of eleven patient-reported QIs for OA consultations were formed. RUG members then suggested the wording and response options of 16 draft items - four QIs were split into two or more questionnaire items to avoid multiple dimensions of care quality within a single item. On comparison of this to the Norwegian OA-QI questionnaire, RUG members commented that both questionnaires contained seven similar QIs. The RUG members and researchers agreed to adopt the Norwegian OA-QI wording for four of these items. RUG members also recommended adopting an additional seven items from the Norwegian OA-QI with some minor word changes to improve their suitability for patients in the UK. One other item from the draft OA QI (UK) questionnaire was retained and eight items were excluded, resulting in a 15-item final version. Conclusions This study describes the development of patient-reported quality indicators for OA primary care derived by members of a RUG group, working in partnership with the research team throughout the study. The OA QI (UK) supports the NICE quality standards for OA and they have been successfully used to assess the quality of OA consultations in primary care in the MOSAICS study. The OA QI (UK) has the potential for routine use in primary care to assess the quality of OA care provided to patients. Ongoing research using both the UK and Norwegian OA-QI questionnaires is assessing the self-reported quality of OA care in different European populations.

Draft assembly of elite inbred line PH207 provides insights into genomic and transcriptome diversity in maize

USDA-ARS?s Scientific Manuscript database

Intense artificial selection over the last 100 years has produced elite maize (Zea mays) inbred lines that combine to produce high-yielding hybrids. To further our understanding of how genome and transcriptome variation contribute to the production of high-yielding hybrids, we generated a draft geno...

75 FR 29357 - Notice of Availability of the Draft Programmatic Environmental Assessment for the Deployment and...

Federal Register 2010, 2011, 2012, 2013, 2014

2010-05-25

... technology that is used to aid in inspecting high-density cargo containers for contraband such as illicit... DEPARTMENT OF HOMELAND SECURITY U.S. Customs and Border Protection Notice of Availability of the Draft Programmatic Environmental Assessment for the Deployment and Operation of High Energy X-Ray...

77 FR 50155 - Neal Smith National Wildlife Refuge, Jasper County, IA

Federal Register 2010, 2011, 2012, 2013, 2014

2012-08-20

... draft plan. In addition, we will use special mailings, newspaper articles, internet postings, and other... quality on prairie and savanna remnants and monitoring and learning from the results of management actions...

An Exploratory Study: Assessment of Modeled Dioxin ...

EPA Pesticide Factsheets

EPA has released an external review draft entitled, An Exploratory Study: Assessment of Modeled Dioxin Exposure in Ceramic Art Studios(External Review Draft). The public comment period and the external peer-review workshop are separate processes that provide opportunities for all interested parties to comment on the document. In addition to consideration by EPA, all public comments submitted in accordance with this notice will also be forwarded to EPA’s contractor for the external peer-review panel prior to the workshop. EPA has realeased this draft document solely for the purpose of pre-dissemination peer review under applicable information quality guidelines. This document has not been formally disseminated by EPA. It does not represent and should not be construed to represent any Agency policy or determination. The purpose of this report is to describe an exploratory investigation of potential dioxin exposures to artists/hobbyists who use ball clay to make pottery and related products.

A Web-Based Search Service to Support Imaging Spectrometer Instrument Operations

NASA Technical Reports Server (NTRS)

Smith, Alexander; Thompson, David R.; Sayfi, Elias; Xing, Zhangfan; Castano, Rebecca

2013-01-01

Imaging spectrometers yield rich and informative data products, but interpreting them demands time and expertise. There is a continual need for new algorithms and methods for rapid first-draft analyses to assist analysts during instrument opera-tions. Intelligent data analyses can summarize scenes to draft geologic maps, searching images to direct op-erator attention to key features. This validates data quality while facilitating rapid tactical decision making to select followup targets. Ideally these algorithms would operate in seconds, never grow bored, and be free from observation bias about the kinds of mineral-ogy that will be found.

Louisiana Coastal Area, Louisiana. Freshwater Diversion to Barataria and Breton Sound Basins. Feasibility Study. Volume 1. Draft Main Report. Draft Environmental Impact Statement.

DTIC Science & Technology

1984-09-01

and shellfish that depend on these organisms would increase. Sport fishing and hunting is related to availability of fish and wild - life resources...colaiLivuly poor quality of the Misissippi R<ivyer wiite ,r oi Lit 1 c i vi iin wate bodil s aud as socia ted fish 6 id wild Ii e resolnrces; ind( ?h pot it...zone between fresh and brackish marsh. Common vegetation in this type includes wiregrass, deerpea, bulltongue, wild millet, bullwhip, and sawgrass. A

Design/Drafting (Graphic Communications). Industrial Arts, Senior High--Level II. North Dakota Senior High Industrial Arts Curriculum Guides.

ERIC Educational Resources Information Center

Poehls, Eddie; And Others

This course guide for a design/drafting course is one of four developed for the graphic communications area in the North Dakota senior high industrial arts education program. (Eight other guides are available for two other areas of Industrial Arts--energy/power and production.) Part 1 provides such introductory information as a definition and…

78 FR 16483 - Notice of Availability of the Draft Uranium Leasing Program Programmatic Environmental Impact...

Federal Register 2010, 2011, 2012, 2013, 2014

2013-03-15

...The U.S. Department of Energy (DOE) announces the availability of the Draft Uranium Leasing Program Programmatic Environmental Impact Statement (Draft ULP PEIS, DOE/EIS-0472D), for public comment. DOE is also announcing the dates, times, and locations for public hearings to receive comments on the Draft ULP PEIS. DOE has prepared the Draft ULP PEIS pursuant to the National Environmental Policy Act of 1969 (NEPA), the Council on Environmental Quality's (CEQ's) NEPA regulations (40 CFR parts 1500-1508), and DOE's NEPA implementing procedures (10 CFR part 1021) to analyze the reasonably foreseeable potential environmental impacts, including the site-specific impacts, of the range of reasonable alternatives for the management of the ULP. DOE's ULP administers 31 tracts of land covering an aggregate of approximately 25,000 acres (10,000 ha) in Mesa, Montrose, and San Miguel Counties in western Colorado for exploration, mine development, operations, and reclamation of uranium mines. The cooperating agencies on this ULP PEIS are the: U.S. Department of the Interior (DOI), Bureau of Land Management (BLM); U.S. Environmental Protection Agency (EPA); Colorado Department of Transportation; Colorado Division of Reclamation, Mining, and Safety; Colorado Parks and Wildlife ; Mesa County Commission; Montrose County Commission; San Juan County Commission; San Miguel County Board of Commissioners; Pueblo of Acoma Tribe; Pueblo de Cochiti Tribe; Pueblo de Isleta Tribe; Navajo Nation; and Southern Ute Indian Tribe.

Numerical Simulation and Validation of a High Head Model Francis Turbine at Part Load Operating Condition

NASA Astrophysics Data System (ADS)

Goyal, Rahul; Trivedi, Chirag; Kumar Gandhi, Bhupendra; Cervantes, Michel J.

2017-07-01

Hydraulic turbines are operated over an extended operating range to meet the real time electricity demand. Turbines operated at part load have flow parameters not matching the designed ones. This results in unstable flow conditions in the runner and draft tube developing low frequency and high amplitude pressure pulsations. The unsteady pressure pulsations affect the dynamic stability of the turbine and cause additional fatigue. The work presented in this paper discusses the flow field investigation of a high head model Francis turbine at part load: 50% of the rated load. Numerical simulation of the complete turbine has been performed. Unsteady pressure pulsations in the vaneless space, runner, and draft tube are investigated and validated with available experimental data. Detailed analysis of the rotor stator interaction and draft tube flow field are performed and discussed. The analysis shows the presence of a rotating vortex rope in the draft tube at the frequency of 0.3 times of the runner rotational frequency. The frequency of the vortex rope precession, which causes severe fluctuations and vibrations in the draft tube, is predicted within 3.9% of the experimental measured value. The vortex rope results pressure pulsations propagating in the system whose frequency is also perceive in the runner and upstream the runner.

The draft genome sequence and annotation of the desert woodrat Neotoma lepida.

PubMed

Campbell, Michael; Oakeson, Kelly F; Yandell, Mark; Halpert, James R; Dearing, Denise

2016-09-01

We present the de novo draft genome sequence for a vertebrate mammalian herbivore, the desert woodrat (Neotoma lepida). This species is of ecological and evolutionary interest with respect to ingestion, microbial detoxification and hepatic metabolism of toxic plant secondary compounds from the highly toxic creosote bush (Larrea tridentata) and the juniper shrub (Juniperus monosperma). The draft genome sequence and annotation have been deposited at GenBank under the accession LZPO01000000.

Integrated editing system for Japanese text and image information "Linernote"

NASA Astrophysics Data System (ADS)

Tanaka, Kazuto

Integrated Japanese text editing system "Linernote" developed by Toyo Industries Co. is explained. The system has been developed on the concept of electronic publishing. It is composed of personal computer NEC PC-9801 VX and other peripherals. Sentence, drawing and image data is inputted and edited under the integrated operating environment in the system and final text is printed out by laser printer. Handling efficiency of time consuming work such as pattern input or page make up has been improved by draft image data indication method on CRT. It is the latest DTP system equipped with three major functions, namly, typesetting for high quality text editing, easy drawing/tracing and high speed image processing.

77 FR 2096 - Notice of Availability of Draft Environmental Impact Statement for the Proposed International...

Federal Register 2010, 2011, 2012, 2013, 2014

2012-01-13

... and scenic resources, climatology, meteorology and air quality, geology, minerals and soils, water... human communities would mostly be small, with the exception of small to moderate impacts on climatology...

Draft genome sequence of Coniochaeta ligniaria NRRL 30616, a lignocellulolytic fungus for bioabatement of inhibitors in plant biomass hydrolysates

USDA-ARS?s Scientific Manuscript database

Here, we report the first draft genome sequence (42.38 Mb that contains 13,657 genes) of Coniochaeta ligniaria NRRL30616, an ascomycete with high biotechnological relevance in the bioenergy field given its high potential for bioabatement of toxic furanic compounds in plant biomass hydrolysates and i...

Teaching Pharmacology Graduate Students how to Write an NIH Grant Application

PubMed Central

O’Donnell, Lauren A.; Surratt, Christopher K.

2015-01-01

Objective. To fill the gap in grant writing training in pharmacology graduate education using an active-learning strategy. Design. Graduate students wrote subsections of a grant according to NIH guidelines. Students revised their applications based on multiple rounds of critiques from professors and peers throughout a semester-long scientific writing course. Assessment. Prerevision and postrevision grant drafts were graded. Students were provided with questionnaires assessing their perception of the process. To determine the impact of feedback on the proposals, the quality of the pre/postrevision drafts was assessed by professors who were blinded and unaffiliated with the course. Conclusion. Student grades improved significantly upon resubmission. Perceptions of the proposals by blinded faculty members favored revised submissions based on multiple criteria. Survey feedback indicated an increase in student confidence in grant writing ability. The results of 3 independent measures demonstrate that intensive feedback on scientific writing improved the quality of student proposals. PMID:28435165

Teaching Pharmacology Graduate Students how to Write an NIH Grant Application.

PubMed

Leak, Rehana K; O'Donnell, Lauren A; Surratt, Christopher K

2015-11-25

Objective. To fill the gap in grant writing training in pharmacology graduate education using an active-learning strategy. Design. Graduate students wrote subsections of a grant according to NIH guidelines. Students revised their applications based on multiple rounds of critiques from professors and peers throughout a semester-long scientific writing course. Assessment. Prerevision and postrevision grant drafts were graded. Students were provided with questionnaires assessing their perception of the process. To determine the impact of feedback on the proposals, the quality of the pre/postrevision drafts was assessed by professors who were blinded and unaffiliated with the course. Conclusion. Student grades improved significantly upon resubmission. Perceptions of the proposals by blinded faculty members favored revised submissions based on multiple criteria. Survey feedback indicated an increase in student confidence in grant writing ability. The results of 3 independent measures demonstrate that intensive feedback on scientific writing improved the quality of student proposals.

Contribution of draft cattle to rural livelihoods in a district of southeastern Uganda endemic for bovine parasitic diseases: an economic evaluation.

PubMed

Okello, Walter O; Muhanguzi, Dennis; MacLeod, Ewan T; Welburn, Susan C; Waiswa, Charles; Shaw, Alexandra P

2015-11-05

A study was conducted in Tororo District in eastern Uganda to assess the socio-economic contribution of draft cattle to rural livelihoods. The aim of the study was to empirically quantify the economic value of draft cattle thus contributing to understanding the impact of endemic parasitic diseases of cattle on livestock productivity and subsequently household income, labor and food security. A total of 205 draft cattle keeping households (n = 205) were randomly selected and structured household questionnaires were administered, focusing on work oxen use, productivity, inputs and outputs. The data obtained was analyzed using standard statistical methods and used to calculate the gross margin from the draft cattle enterprise. Secondary data were obtained from focus group discussions and key informant interviews and these were analyzed using Bayesian methods. The study showed that, apart from being labor saving, the use of animal traction is highly profitable with the gross margin per year from the use of draft cattle amounting to 245 United States dollars per work oxen owning household. The cash obtained from hiring out draft animals was equivalent to nearly a quarter of the average local household's monetary receipts. It also revealed that endemic bovine parasitic diseases such as trypanosomiasis and tick-borne diseases reduced draft cattle output by 20.9 % and potential household income from the use of draft oxen by 32.2 %. The presence of endemic cattle diseases in rural Uganda is adversely affecting the productivity of draft cattle, which in turn affects household income, labor and ultimately food security. This study highlights the contribution of draft cattle to rural livelihoods, thus increasing the expected impact of cost-effective control strategies of endemic production limiting livestock diseases in Uganda.

IRIS Toxicological Review of Ethyl Tertiary Butyl Ether (Etbe) ...

EPA Pesticide Factsheets

In August 2013, EPA released the draft literature searches and associated search strategies, evidence tables, and exposure response arrays for ETBE to obtain input from stakeholders and the public prior to developing the draft IRIS assessment. Specifically, EPA was interested in comments on the following: Draft literature search strategies The approach for identifying studies The screening process for selecting pertinent studies The resulting list of pertinent studies Preliminary evidence tables The process for selecting studies to include in evidence tables The quality of the studies in the evidence tables The literature search strategy, which describes the processes for identifying scientific literature, contains the studies that EPA considered and selected to include in the evidence tables. The preliminary evidence tables and exposure-response arrays present the key study data in a standardized format. The evidence tables summarize the available critical scientific literature. The exposure-response figures provide a graphical representation of the responses at different levels of exposure for each study in the evidence table. The draft Toxicological Review of Ethyl Tertiary Butyl Ether provides scientific support and rationale for the hazard and dose-response assessment pertaining to chronic exposure to ethyl tertiary butyl ether.

SSPACE-LongRead: scaffolding bacterial draft genomes using long read sequence information

PubMed Central

2014-01-01

Background The recent introduction of the Pacific Biosciences RS single molecule sequencing technology has opened new doors to scaffolding genome assemblies in a cost-effective manner. The long read sequence information is promised to enhance the quality of incomplete and inaccurate draft assemblies constructed from Next Generation Sequencing (NGS) data. Results Here we propose a novel hybrid assembly methodology that aims to scaffold pre-assembled contigs in an iterative manner using PacBio RS long read information as a backbone. On a test set comprising six bacterial draft genomes, assembled using either a single Illumina MiSeq or Roche 454 library, we show that even a 50× coverage of uncorrected PacBio RS long reads is sufficient to drastically reduce the number of contigs. Comparisons to the AHA scaffolder indicate our strategy is better capable of producing (nearly) complete bacterial genomes. Conclusions The current work describes our SSPACE-LongRead software which is designed to upgrade incomplete draft genomes using single molecule sequences. We conclude that the recent advances of the PacBio sequencing technology and chemistry, in combination with the limited computational resources required to run our program, allow to scaffold genomes in a fast and reliable manner. PMID:24950923

High-quality draft genome sequence of Flavobacterium suncheonense GH29-5 T (DSM 17707 T) isolated from greenhouse soil in South Korea, and emended description of Flavobacterium suncheonense GH29-5 T

DOE PAGES

Tashkandy, Nisreen; Sabban, Sari; Fakieh, Mohammad; ...

2016-06-16

Flavobacterium suncheonense is a member of the family Flavobacteriaceae in the phylum Bacteroidetes. Strain GH29-5 T (DSM 17707 T ) was isolated from greenhouse soil in Suncheon, South Korea. F. suncheonense GH29-5 T is part of the Genomic Encyclopedia of Bacteria and Archaea project. The 2,880,663 bp long draft genome consists of 54 scaffolds with 2739 protein-coding genes and 82 RNA genes. The genome of strain GH29-5 T has 117 genes encoding peptidases but a small number of genes encoding carbohydrate active enzymes (51 CAZymes). Metallo and serine peptidases were found most frequently. Among CAZymes, eight glycoside hydrolase families, ninemore » glycosyl transferase families, two carbohydrate binding module families and four carbohydrate esterase families were identified. Suprisingly, polysaccharides utilization loci (PULs) were not found in strain GH29-5 T . Based on the coherent physiological and genomic characteristics we suggest that F. suncheonense GH29-5 T feeds rather on proteins than saccharides and lipids.« less

Two Low Coverage Bird Genomes and a Comparison of Reference-Guided versus De Novo Genome Assemblies

PubMed Central

Card, Daren C.; Schield, Drew R.; Reyes-Velasco, Jacobo; Fujita, Matthew K.; Andrew, Audra L.; Oyler-McCance, Sara J.; Fike, Jennifer A.; Tomback, Diana F.; Ruggiero, Robert P.; Castoe, Todd A.

2014-01-01

As a greater number and diversity of high-quality vertebrate reference genomes become available, it is increasingly feasible to use these references to guide new draft assemblies for related species. Reference-guided assembly approaches may substantially increase the contiguity and completeness of a new genome using only low levels of genome coverage that might otherwise be insufficient for de novo genome assembly. We used low-coverage (∼3.5–5.5x) Illumina paired-end sequencing to assemble draft genomes of two bird species (the Gunnison Sage-Grouse, Centrocercus minimus, and the Clark's Nutcracker, Nucifraga columbiana). We used these data to estimate de novo genome assemblies and reference-guided assemblies, and compared the information content and completeness of these assemblies by comparing CEGMA gene set representation, repeat element content, simple sequence repeat content, and GC isochore structure among assemblies. Our results demonstrate that even lower-coverage genome sequencing projects are capable of producing informative and useful genomic resources, particularly through the use of reference-guided assemblies. PMID:25192061

The Transcriptomics of Secondary Growth and Wood Formation in Conifers

PubMed Central

Carvalho, Ana; Paiva, Jorge; Louzada, José; Lima-Brito, José

2013-01-01

In the last years, forestry scientists have adapted genomics and next-generation sequencing (NGS) technologies to the search for candidate genes related to the transcriptomics of secondary growth and wood formation in several tree species. Gymnosperms, in particular, the conifers, are ecologically and economically important, namely, for the production of wood and other forestry end products. Until very recently, no whole genome sequencing of a conifer genome was available. Due to the gradual improvement of the NGS technologies and inherent bioinformatics tools, two draft assemblies of the whole genomes sequence of Picea abies and Picea glauca arose in the current year. These draft genome assemblies will bring new insights about the structure, content, and evolution of the conifer genomes. Furthermore, new directions in the forestry, breeding and research of conifers will be discussed in the following. The identification of genes associated with the xylem transcriptome and the knowledge of their regulatory mechanisms will provide less time-consuming breeding cycles and a high accuracy for the selection of traits related to wood production and quality. PMID:24288610

Draft De Novo Transcriptome of the Rat Kangaroo Potorous tridactylus as a Tool for Cell Biology

PubMed Central

Udy, Dylan B.; Voorhies, Mark; Chan, Patricia P.; Lowe, Todd M.; Dumont, Sophie

2015-01-01

The rat kangaroo (long-nosed potoroo, Potorous tridactylus) is a marsupial native to Australia. Cultured rat kangaroo kidney epithelial cells (PtK) are commonly used to study cell biological processes. These mammalian cells are large, adherent, and flat, and contain large and few chromosomes—and are thus ideal for imaging intra-cellular dynamics such as those of mitosis. Despite this, neither the rat kangaroo genome nor transcriptome have been sequenced, creating a challenge for probing the molecular basis of these cellular dynamics. Here, we present the sequencing, assembly and annotation of the draft rat kangaroo de novo transcriptome. We sequenced 679 million reads that mapped to 347,323 Trinity transcripts and 20,079 Unigenes. We present statistics emerging from transcriptome-wide analyses, and analyses suggesting that the transcriptome covers full-length sequences of most genes, many with multiple isoforms. We also validate our findings with a proof-of-concept gene knockdown experiment. We expect that this high quality transcriptome will make rat kangaroo cells a more tractable system for linking molecular-scale function and cellular-scale dynamics. PMID:26252667

High-quality draft genome sequence of Gracilimonas tropica CL-CB462 T (DSM 19535 T), isolated from a Synechococcus culture

DOE PAGES

Choi, Dong Han; Ahn, Chisang; Jang, Gwang Il; ...

2015-11-11

Gracilimonas tropica Choi et al. 2009 is a member of order Sphingobacteriales, class Sphingobacteriia. Three species of the genus Gracilimonas have been isolated from marine seawater or a salt mine and showed extremely halotolerant and mesophilic features, although close relatives are extremely halophilic or thermophilic. The type strain of the type species of Gracilimonas, G. tropica DSM19535 T, was isolated from a Synechococcus culture which was established from the tropical sea-surface water of the Pacific Ocean. The genome of the strain DSM19535 T was sequenced through the Genomic Encyclopedia of Type Strains, Phase I: the one thousand microbial genomes project.more » Here, we describe the genomic features of the strain. The 3,831,242 bp long draft genome consists of 48 contigs with 3373 protein-coding and 53 RNA genes. Finally, the strain seems to adapt to phosphate limitation and requires amino acids from external environment. In addition, genomic analyses and pasteurization experiment suggested that G. tropica DSM19535 T did not form spore.« less

Draft De Novo Transcriptome of the Rat Kangaroo Potorous tridactylus as a Tool for Cell Biology.

PubMed

Udy, Dylan B; Voorhies, Mark; Chan, Patricia P; Lowe, Todd M; Dumont, Sophie

2015-01-01

The rat kangaroo (long-nosed potoroo, Potorous tridactylus) is a marsupial native to Australia. Cultured rat kangaroo kidney epithelial cells (PtK) are commonly used to study cell biological processes. These mammalian cells are large, adherent, and flat, and contain large and few chromosomes-and are thus ideal for imaging intra-cellular dynamics such as those of mitosis. Despite this, neither the rat kangaroo genome nor transcriptome have been sequenced, creating a challenge for probing the molecular basis of these cellular dynamics. Here, we present the sequencing, assembly and annotation of the draft rat kangaroo de novo transcriptome. We sequenced 679 million reads that mapped to 347,323 Trinity transcripts and 20,079 Unigenes. We present statistics emerging from transcriptome-wide analyses, and analyses suggesting that the transcriptome covers full-length sequences of most genes, many with multiple isoforms. We also validate our findings with a proof-of-concept gene knockdown experiment. We expect that this high quality transcriptome will make rat kangaroo cells a more tractable system for linking molecular-scale function and cellular-scale dynamics.

Draft Genome Sequence of Clostridium pasteurianum NRRL B-598, a Potential Butanol or Hydrogen Producer.

PubMed

Kolek, Jan; Sedlár, Karel; Provazník, Ivo; Patáková, Petra

2014-03-20

We present a draft genome sequence of Clostridium pasteurianum NRRL B-598. This strain ferments saccharides by two-stage acetone-butanol (AB) fermentation, is oxygen tolerant, and has high hydrogen yields.

Photographic and drafting techniques simplify method of producing engineering drawings

NASA Technical Reports Server (NTRS)

Provisor, H.

1968-01-01

Combination of photographic and drafting techniques has been developed to simplify the preparation of three dimensional and dimetric engineering drawings. Conventional photographs can be converted to line drawings by making copy negatives on high contrast film.

Publication guidelines for quality improvement in health care: evolution of the SQUIRE project

PubMed Central

Davidoff, F; Batalden, P; Stevens, D; Ogrinc, G; Mooney, S

2008-01-01

In 2005, draft guidelines were published for reporting studies of quality improvement interventions as the initial step in a consensus process for development of a more definitive version. This article contains the full revised version of the guidelines, which the authors refer to as SQUIRE (Standards for QUality Improvement Reporting Excellence). This paper also describes the consensus process, which included informal feedback from authors, editors and peer reviewers who used the guidelines; formal written commentaries; input from a group of publication guideline developers; ongoing review of the literature on the epistemology of improvement and methods for evaluating complex social programmes; a two-day meeting of stakeholders for critical discussion and debate of the guidelines’ content and wording; and commentary on sequential versions of the guidelines from an expert consultant group. Finally, the authors consider the major differences between SQUIRE and the initial draft guidelines; limitations of and unresolved questions about SQUIRE; ancillary supporting documents and alternative versions that are under development; and plans for dissemination, testing and further development of SQUIRE. PMID:18836063

Draft Assembly of Elite Inbred Line PH207 Provides Insights into Genomic and Transcriptome Diversity in Maize

DOE Office of Scientific and Technical Information (OSTI.GOV)

Hirsch, Candice N.; Hirsch, Cory D.; Brohammer, Alex B.

Intense artificial selection over the last 100 years has produced elite maize (Zea mays) inbred lines that combine to produce high-yielding hybrids. To further our understanding of how genome and transcriptome variation contribute to the production of high-yielding hybrids, we generated a draft genome assembly of the inbred line PH207 to complement and compare with the existing B73 reference sequence. B73 is a founder of the Stiff Stalk germplasm pool, while PH207 is a founder of Iodent germplasm, both of which have contributed substantially to the production of temperate commercial maize and are combined to make heterotic hybrids. Comparison ofmore » these two assemblies revealed over 2500 genes present in only one of the two genotypes and 136 gene families that have undergone extensive expansion or contraction. Transcriptome profiling revealed extensive expression variation, with as many as 10,564 differentially expressed transcripts and 7128 transcripts expressed in only one of the two genotypes in a single tissue. Genotype-specific genes were more likely to have tissue/condition-specific expression and lower transcript abundance. The availability of a high-quality genome assembly for the elite maize inbred PH207 expands our knowledge of the breadth of natural genome and transcriptome variation in elite maize inbred lines across heterotic pools.« less

Draft Assembly of Elite Inbred Line PH207 Provides Insights into Genomic and Transcriptome Diversity in Maize

DOE PAGES

Hirsch, Candice N.; Hirsch, Cory D.; Brohammer, Alex B.; ...

2016-11-01

Intense artificial selection over the last 100 years has produced elite maize (Zea mays) inbred lines that combine to produce high-yielding hybrids. To further our understanding of how genome and transcriptome variation contribute to the production of high-yielding hybrids, we generated a draft genome assembly of the inbred line PH207 to complement and compare with the existing B73 reference sequence. B73 is a founder of the Stiff Stalk germplasm pool, while PH207 is a founder of Iodent germplasm, both of which have contributed substantially to the production of temperate commercial maize and are combined to make heterotic hybrids. Comparison ofmore » these two assemblies revealed over 2500 genes present in only one of the two genotypes and 136 gene families that have undergone extensive expansion or contraction. Transcriptome profiling revealed extensive expression variation, with as many as 10,564 differentially expressed transcripts and 7128 transcripts expressed in only one of the two genotypes in a single tissue. Genotype-specific genes were more likely to have tissue/condition-specific expression and lower transcript abundance. The availability of a high-quality genome assembly for the elite maize inbred PH207 expands our knowledge of the breadth of natural genome and transcriptome variation in elite maize inbred lines across heterotic pools.« less

Draft Assembly of Elite Inbred Line PH207 Provides Insights into Genomic and Transcriptome Diversity in Maize[OPEN

PubMed Central

Soifer, Ilya; Barad, Omer; Shem-Tov, Doron; Baruch, Kobi; Lu, Fei; Hernandez, Alvaro G.; Wright, Chris L.; Koehler, Klaus; Buell, C. Robin; de Leon, Natalia

2016-01-01

Intense artificial selection over the last 100 years has produced elite maize (Zea mays) inbred lines that combine to produce high-yielding hybrids. To further our understanding of how genome and transcriptome variation contribute to the production of high-yielding hybrids, we generated a draft genome assembly of the inbred line PH207 to complement and compare with the existing B73 reference sequence. B73 is a founder of the Stiff Stalk germplasm pool, while PH207 is a founder of Iodent germplasm, both of which have contributed substantially to the production of temperate commercial maize and are combined to make heterotic hybrids. Comparison of these two assemblies revealed over 2500 genes present in only one of the two genotypes and 136 gene families that have undergone extensive expansion or contraction. Transcriptome profiling revealed extensive expression variation, with as many as 10,564 differentially expressed transcripts and 7128 transcripts expressed in only one of the two genotypes in a single tissue. Genotype-specific genes were more likely to have tissue/condition-specific expression and lower transcript abundance. The availability of a high-quality genome assembly for the elite maize inbred PH207 expands our knowledge of the breadth of natural genome and transcriptome variation in elite maize inbred lines across heterotic pools. PMID:27803309

78 FR 5492 - Draft Environmental Impact Statement for Merced Wild and Scenic River Comprehensive Management...

Federal Register 2010, 2011, 2012, 2013, 2014

2013-01-25

...), and the Council on Environmental Quality Regulations (40 CFR part 1500-1508), the National Park... are protected and enhanced over the life of the plan. The Merced River Plan/DEIS evaluates the...

75 FR 57051 - Notice of Intent To Prepare a Draft Environmental Impact Statement for the Sunset Area Community...

Federal Register 2010, 2011, 2012, 2013, 2014

2010-09-17

... community, a Renton Housing Authority property of approximately 100 existing units in 50-year old, two story... quality, including greenhouse gas emissions; earth; energy; environmental health; environmental justice...

78 FR 4134 - Availability of the Draft Finding of No Significant Impact and Final Programmatic Environmental...

Federal Register 2010, 2011, 2012, 2013, 2014

2013-01-18

... restructuring. This range of potential installation reduction and growth (ranging from maximum losses of 8,000... with implementation of the two Action alternatives include impacts to air quality; airspace; cultural...

75 FR 69983 - Notice of Intent to Prepare a Draft Environmental Impact Statement (EIS), Initiate the Public...

Federal Register 2010, 2011, 2012, 2013, 2014

2010-11-16

... that water quality has improved, these canals allow the transfer of both indigenous and nonindigenous... Water and Related Land Resource Implementation Studies, Water Resources Council, March 10, 1983. 2...

78 FR 16656 - Draft Damage Assessment and Restoration Plan and Environmental Assessment for Natural Resource...

Federal Register 2010, 2011, 2012, 2013, 2014

2013-03-18

... may be sent to: Ian Zelo, NOAA Oil Spill Coordinator, Assessment and Restoration Division, 7600 Sand... are: (1) Improve Helmet Creek, restore juvenile and adult fish passage, (2) Improve water quality, and...

Research Matters

ERIC Educational Resources Information Center

VanDeWeghe, Rick

2005-01-01

The critical role played by the teacher feedback on the students' drafts is discussed. The standards-based scales measuring content, organization, and mechanics was used to determine the quality of students' writing and the teachers' feedback commentaries were considered either content level or surface level.

Experimental investigation of the draft tube inlet flow of a bulb turbine

NASA Astrophysics Data System (ADS)

Vuillemard, J.; Aeschlimann, V.; Fraser, R.; Lemay, S.; Deschênes, C.

2014-03-01

In the BulbT project framework, a bulb turbine model was studied with a strongly diverging draft tube. At high discharge, flow separation occurs in the draft tube correlated to significant efficiency and power drops. In this context, a focus was put on the draft tube inlet flow conditions. Actually, a precise inlet flow velocity field is required for comparison and validation purposes with CFD simulation. This paper presents different laser Doppler velocimetry (LDV) measurements at the draft tube inlet and their analysis. The LDV was setup to measure the axial and circumferential velocity on a radius under the runner and a diameter under the hub. A method was developed to perform indirect measurement of the mean radial velocity component. Five operating conditions were studied to correlate the inlet flow to the separation in the draft tube. Mean velocities, fluctuations and frequencies allowed characterizing the flow. Using this experimental database, the flow structure was characterized. Phase averaged velocities based on the runner position allowed detecting the runner blade wakes. The velocity gradients induced by the blade tip vortices were captured. The guide vane wakes was also detected at the draft tube inlet. The recirculation in the hub wake was observed.

Field Results for an Arctic AUV Designed for Characterizing Circulation and Ice Thickness

NASA Astrophysics Data System (ADS)

Bellingham, J. G.; Kirkwood, W. J.; Tervalon, N.; Cokelet, E.; Thomas, H.; Sibenac, M.; Gashler, D.; McEwen, R.; Henthorn, R.; Shane, F.; Osborn, D. J.; Johnson, K.; Overland, J.; Stein, P.; Bahlavouni, A.; Anderson, D.

2002-12-01

An Autonomous Underwater Vehicle designed for operation at high latitudes and under ice completed its first Arctic field tests from the USCGC Healy in fall of 2001. The ALTEX AUV has been under development since 1998, and is being created to provide: unprecedented endurance, ability to navigate at high latitudes, a depth rating of 1500 to 4500 meters depending on payload, and the capability to relay data through the ice to satellites via data buoys. The AUV's initial applications are focused on tracking the warm Atlantic Layer inflow - the primary source of seawater to the Arctic Ocean. Consequently the primary payloads are twin pumped CTD systems. Oxygen and nitrate sensors provide the ability to use NO as a tracer. An ice profiling sonar allows the AUV to estimate the ice thickness in real-time and is designed to generate high quality post-processed ice draft data comparable to that collected through the SCICEX program. The experiments in October aboard the USCGC Healy generated numerous water column and under-ice data sets. Traditional ship-based CTD operations were used to provide a comparison data set for AUV water column measurements. The post-processed ice draft results show reasonable ice profiles and have the potential, when combined with other science data collected, to shed some additional light on upper water column processes in ice-covered regions. Cruise results include: operating the AUV from the USCGC Healy in the ice pack, demonstrating inertial navigation system performance, obtaining oceanographic sections with the AUV, obtaining ice draft measurements with an AUV born sonar, and testing the data-buoy system. This work is supported by the National Science Foundation under grant NSF-OPP 9910290. The Packard Foundation and the Office of Naval Research have also provided support. The project was initiated under the National Ocean Partnership Program under contract N00014-98-1-0814.

The draft genome sequence of cork oak

PubMed Central

Ramos, António Marcos; Usié, Ana; Barbosa, Pedro; Barros, Pedro M.; Capote, Tiago; Chaves, Inês; Simões, Fernanda; Abreu, Isabl; Carrasquinho, Isabel; Faro, Carlos; Guimarães, Joana B.; Mendonça, Diogo; Nóbrega, Filomena; Rodrigues, Leandra; Saibo, Nelson J. M.; Varela, Maria Carolina; Egas, Conceição; Matos, José; Miguel, Célia M.; Oliveira, M. Margarida; Ricardo, Cândido P.; Gonçalves, Sónia

2018-01-01

Cork oak (Quercus suber) is native to southwest Europe and northwest Africa where it plays a crucial environmental and economical role. To tackle the cork oak production and industrial challenges, advanced research is imperative but dependent on the availability of a sequenced genome. To address this, we produced the first draft version of the cork oak genome. We followed a de novo assembly strategy based on high-throughput sequence data, which generated a draft genome comprising 23,347 scaffolds and 953.3 Mb in size. A total of 79,752 genes and 83,814 transcripts were predicted, including 33,658 high-confidence genes. An InterPro signature assignment was detected for 69,218 transcripts, which represented 82.6% of the total. Validation studies demonstrated the genome assembly and annotation completeness and highlighted the usefulness of the draft genome for read mapping of high-throughput sequence data generated using different protocols. All data generated is available through the public databases where it was deposited, being therefore ready to use by the academic and industry communities working on cork oak and/or related species. PMID:29786699

The draft genome sequence of cork oak.

PubMed

Ramos, António Marcos; Usié, Ana; Barbosa, Pedro; Barros, Pedro M; Capote, Tiago; Chaves, Inês; Simões, Fernanda; Abreu, Isabl; Carrasquinho, Isabel; Faro, Carlos; Guimarães, Joana B; Mendonça, Diogo; Nóbrega, Filomena; Rodrigues, Leandra; Saibo, Nelson J M; Varela, Maria Carolina; Egas, Conceição; Matos, José; Miguel, Célia M; Oliveira, M Margarida; Ricardo, Cândido P; Gonçalves, Sónia

2018-05-22

Cork oak (Quercus suber) is native to southwest Europe and northwest Africa where it plays a crucial environmental and economical role. To tackle the cork oak production and industrial challenges, advanced research is imperative but dependent on the availability of a sequenced genome. To address this, we produced the first draft version of the cork oak genome. We followed a de novo assembly strategy based on high-throughput sequence data, which generated a draft genome comprising 23,347 scaffolds and 953.3 Mb in size. A total of 79,752 genes and 83,814 transcripts were predicted, including 33,658 high-confidence genes. An InterPro signature assignment was detected for 69,218 transcripts, which represented 82.6% of the total. Validation studies demonstrated the genome assembly and annotation completeness and highlighted the usefulness of the draft genome for read mapping of high-throughput sequence data generated using different protocols. All data generated is available through the public databases where it was deposited, being therefore ready to use by the academic and industry communities working on cork oak and/or related species.

Community-led comparative genomic and phenotypic analysis of the aquaculture pathogen Pseudomonas baetica a390T sequenced by Ion semiconductor and Nanopore technologies

PubMed Central

Beaton, Ainsley; Lood, Cédric; Cunningham-Oakes, Edward; MacFadyen, Alison; Mullins, Alex J; Bestawy, Walid El; Botelho, João; Chevalier, Sylvie; Dalzell, Chloe; Dolan, Stephen K; Faccenda, Alberto; Ghequire, Maarten G K; Higgins, Steven; Kutschera, Alexander; Murray, Jordan; Redway, Martha; Salih, Talal; Smith, Brian A; Smits, Nathan; Thomson, Ryan; Woodcock, Stuart; Cornelis, Pierre; Lavigne, Rob; van Noort, Vera

2018-01-01

Abstract Pseudomonas baetica strain a390T is the type strain of this recently described species and here we present its high-contiguity draft genome. To celebrate the 16th International Conference on Pseudomonas, the genome of P. baetica strain a390T was sequenced using a unique combination of Ion Torrent semiconductor and Oxford Nanopore methods as part of a collaborative community-led project. The use of high-quality Ion Torrent sequences with long Nanopore reads gave rapid, high-contiguity and -quality, 16-contig genome sequence. Whole genome phylogenetic analysis places P. baetica within the P. koreensis clade of the P. fluorescens group. Comparison of the main genomic features of P. baetica with a variety of other Pseudomonas spp. suggests that it is a highly adaptable organism, typical of the genus. This strain was originally isolated from the liver of a diseased wedge sole fish, and genotypic and phenotypic analyses show that it is tolerant to osmotic stress and to oxytetracycline. PMID:29579234

78 FR 47241 - Amendments to the Water Quality Regulations, Water Code and Comprehensive Plan To Revise the...

Federal Register 2010, 2011, 2012, 2013, 2014

2013-08-05

...The Delaware River Basin Commission (``DRBC'' or ``Commission'') will hold a public hearing to receive comments on proposed amendments to the Commission's Water Quality Regulations, Water Code and Comprehensive Plan to revise the water quality criteria for polychlorinated biphenyls (``PCBs'') in the Delaware Estuary and Bay, DRBC Water Quality Management Zones 2 through 6, for the protection of human health from carcinogenic effects. The Commission will simultaneously solicit comment on a draft implementation strategy to support achievement of the criteria.

75 FR 54871 - Science Advisory Board Staff Office; Notification of a Clean Air Scientific Advisory Committee...

Federal Register 2010, 2011, 2012, 2013, 2014

2010-09-09

... Scientific Advisory Committee NO X and SO X Secondary National Ambient Air Quality Standards (NAAQS) Review... Ambient Air Quality Standards for NO X and SO X : Second External Review Draft (September 2010). DATES... (FACA), Public Law 92-463 5 U.S.C., App. 2, notice is hereby given that the CASAC NO X & SO X Secondary...

PRINTQUAL - a measure for assessing the quality of newspaper reporting of suicide.

PubMed

John, Ann; Hawton, Keith; Lloyd, Keith; Luce, Ann; Platt, Stephen; Scourfield, Jonathan; Marchant, Amanda L; Jones, Phil A; Dennis, Mick S

2014-01-01

Many studies have demonstrated a relationship between newspaper reporting of actual or fictional suicides and subsequent suicidal behaviors. Previous measures of the quality of reporting lack consistency concerning which specific elements should be included and how they should be weighted. To develop an instrument, PRINTQUAL, comprising two scales of the quality (poor and good) of newspaper reporting of suicide that can be used in future studies of reporting. A first draft of the PRINTQUAL instrument was compiled, comprising items indicative of poor- and good-quality newspaper reporting based on guidelines and key sources of evidence. This was refined by team members and then circulated to a group of international experts in the field for further opinion and weighting of individual items. The final instrument comprised 19 items in the poor-quality scale and four in the good-quality scale. Following training, agreement between raters was acceptably high for most items (κ ≥ .75) except for three items for which agreement was still acceptable (κ ≥ .60). The PRINTQUAL instrument for assessing the quality of newspaper reporting of suicide appears appropriate for use in research and monitoring in future studies.

Experimental analysis of the flow pattern of a pump turbine model in pump mode

NASA Astrophysics Data System (ADS)

Guggenberger, Mark; Senn, Florian; Jaberg, Helmut; Gehrer, Arno; Sallaberger, Manfred; Widmer, Christian

2016-11-01

Reversible pump turbines are the only means to store primary energy in an highly efficient way. Within a short time their operation can be switched between the different operational regimes thus enhancing the stabilization of the electric grid. These qualities in combination with the operation even at off-design conditions offer a high flexibility to the energy market. However, pump turbines pass through operational regimes where their behaviour becomes unstable. One of these effects occurs when the flowrate is decreased continuously down to a minimum. This point is the physical limitation of the pump operation and is very difficult to predict properly by numerical design without a model test. The purpose of the present study is to identify the fluid mechanical phenomena leading to the occurrence of instabilities of pump turbines in pump mode. A reduced scale model of a ANDRITZ pump turbine was installed on a 4-quadrant test rig for the experimental investigation of unstable conditions in pump mode. The performed measurements are based on the IEC60193-standard. Characteristic measurements at a single guide vane opening were carried out to get a detailed insight into the instabilities in pump mode. The interaction between runner and guide vane was analysed by Particle Image Velocimetry. Furthermore, high-speed visualizations of the suction side part load flow and the suction recirculation were performed. Like never before the flow pattern in the draft tube cone became visible with the help of a high-speed camera by intentionally caused cavitation effects which allow a qualitative view on the flow pattern in the draft tube cone. Suction recirculation is observed in form of single vortices separating from each runner blade and stretching into the draft tube against the main flow direction. To find an explanation for the flow phenomena responsible for the appearance of the unstable head curve also characteristic velocity distributions on the pressure side were combined with high-speed visualizations on the suction side of the pump turbine model. The results enhance the comprehension of the physical background leading to the instability and improve the numerical predictability of the instability in pump mode.

32 CFR 989.19 - Draft EIS.

Code of Federal Regulations, 2010 CFR

2010-07-01

... summary to the public with an attached list of locations (such as public libraries) where the entire draft... a proposed action will potentially have disproportionately high and adverse human health or... as when there has been a significant change in circumstances, development of significant new...

U.S. Draft Domestic Action Plan for Lake Erie

EPA Pesticide Factsheets

In 2016, in response to the 2012 Great Lakes Water Quality Agreement commitments, Canada and the U.S. adopted phosphorus reduction targets for Lake Erie. Each country is developing domestic action plans which outline strategies for meeting the new targets.

LOOP marine and estuarine monitoring program, 1978-95 : volume 6 : sediment quality.

DOT National Transportation Integrated Search

1998-01-01

The Louisiana Offshore Oil Port (LOOP) facilities in coastal Louisiana provide the United States with the country's only Superport for off-loading deep draft tankers. Sediment samples were collected quarterly from 1970 through 1995 at designated stat...

[Applying the Modified Delphi Technique to Develop the Role of HIV Case Managers and Essential Nursing Competencies in HIV Care].

PubMed

Ko, Nai-Ying; Hsieh, Chia-Yin; Chen, Yen-Chin; Tsai, Chen-Hsi; Liu, Hsiao-Ying; Liu, Li-Fang

2015-08-01

Since 2005, the Taiwan Centers for Disease Control (Taiwan CDC) initiated an HIV case management program in AIDS-designated hospitals to provide integrative services and risk-reduction counseling for HIV-infected individuals. In light of the increasingly complex and highly specialized nature of clinical care, expanding and improving competency-based professional education is important to enhance the quality of HIV/AIDS care. The aim of this study was to develop the essential competency framework for HIV care for HIV case managers in Taiwan. We reviewed essential competencies of HIV care from Canada, the United Kingdom, and several African countries and devised descriptions of the roles of case managers and of the associated core competencies for HIV care in Taiwan. The modified Delphi technique was used to evaluate the draft framework of these roles and core competencies. A total of 15 HIV care experts were invited to join the expert panel to review and rank the draft framework. The final framework consisted of 7 roles and 27 competencies for HIV case managers. In Round 1, only 3 items did not receive consensus approval from the experts. After modification based on opinions of the experts, 7 roles and 27 competencies received 97.06% consensus approval in Round 2 and were organized into the final framework for HIV case managers. These roles and associated core competencies were: HIV Care Expert (9 competencies), Communicator (1 competency), Collaborator (4 competencies), Navigator (2 competencies), Manager (4 competencies), Advocate (2 competencies), and Professional (5 competencies). The authors developed an essential competency framework for HIV care using the consensus of a multidisciplinary expert panel. Curriculum developers and advanced nurses and practitioners may use this framework to support developments and to ensure a high quality of HIV care.

Architectural Drafting. Curriculum Development. Bulletin 1779.

ERIC Educational Resources Information Center

Eppler, Thomas

This teaching guide is designed to aid high school vocational education teachers in teaching a course in architectural drafting for students who plan to become architects, interior designers and decorators, architectural draftspersons, landscape engineers, building contractors, building estimators, real estate persons or appraisers, and others.…

Integrated Science Assessment (ISA) for Carbon Monoxide ...

EPA Pesticide Factsheets

EPA announced that the Second External Review Draft of the Integrated Science Assessment (ISA) for Carbon Monoxide (CO) and related Annexes was made available for independent peer review and public review. This draft ISA document represents a concise synthesis and evaluation of the most policy-relevant science and will ultimately provide the scientific bases for EPA's decision regarding whether the current standards for CO sufficiently protect public health and the environment. Section 108(a) of the Clean Air Act directs the EPA Administrator to identify certain pollutants that “cause or contribute to air pollution which may reasonably be anticipated to endanger public health or welfare” and to issue air quality criteria for them. These air quality criteria are to “accurately reflect the latest scientific knowledge useful in indicating the kind and extent of all identifiable effects on public health or welfare which may be expected from the presence of such pollutant in the ambient air….” Under section 109 of the Act, EPA is to establish national ambient air quality standards (NAAQS) for each pollutant for which EPA has issued criteria. Section 109(d) of the Act requires periodic review and, if appropriate, revision of existing air quality criteria to reflect advances in scientific knowledge on the effects of the pollutant on public health or welfare. EPA is also to revise the NAAQS, if appropriate, based on the revised air quality criteria.

Computer-Integrated Manufacturing Technology. Tech Prep Competency Profile.

ERIC Educational Resources Information Center

Lakeland Tech Prep Consortium, Kirtland, OH.

This tech prep competency profile covers these occupations: manufacturing technician, computer-assisted design and drafting (CADD) technician, quality technician, and mechanical technician. Section 1 provides occupation definitions. Section 2 lists development committee members. Section 3 provides the leveling codes---abbreviations for grade level…

21 CFR 211.160 - General requirements.

Code of Federal Regulations, 2010 CFR

2010-04-01

... drafted by the appropriate organizational unit and reviewed and approved by the quality control unit. The...: GENERAL CURRENT GOOD MANUFACTURING PRACTICE FOR FINISHED PHARMACEUTICALS Laboratory Controls § 211.160... procedures, or other laboratory control mechanisms required by this subpart, including any change in such...

Conceptual designs and cost estimates of mechanical draft wet/dry and natural draft dry cooling systems using Curtiss-Wright integral fin-tube heat exchangers

DOE Office of Scientific and Technical Information (OSTI.GOV)

Haberski, R.J.; Bentz, J.C.

1979-04-01

This study was performed to establish a conceptual design and cost evaluation of an advanced technology mechanical draft wet/dry and natural draft dry cooling systems for large electric power plants using a high performance integral fin-tube heat transfer surface. This study was part of an overall DOE program to develop and demonstrate advanced concept cooling systems for large electric power plants. The results obtained show significant economic advantages compared to results previously published for conventional cooling systems. These advantages are due to the higher heat transfer and lower pressure loss which occur with the use of the selected multi-port integralmore » fin-tubes.« less

78 FR 40128 - Notice of Intent to Prepare a Draft Environmental Impact Statement (EIS) for the Reaches 8, 9...

Federal Register 2010, 2011, 2012, 2013, 2014

2013-07-03

... to R-134+135. The Town proposes to place approximately 74,300 cubic yards of beach quality sand in... beach quality sand is proposed to be placed between R-134+135 and R-135+551 to elevate the existing berm... and dune restoration projects between R-128+955 and R-138+551 with sand placement and the construction...

Gloss uniformity measurement update for ISO/IEC 19751

NASA Astrophysics Data System (ADS)

Ng, Yee S.; Cui, Chengwu; Kuo, Chunghui; Maggard, Eric; Mashtare, Dale; Morris, Peter

2005-01-01

To address the standardization issues of perceptually based image quality for printing systems, ISO/IEC JTC1/SC28, the standardization committee for office equipment chartered the W1.1 project with the responsibility of drafting a proposal for an international standard for the evaluation of printed image quality1. An ISO draft Standard2, ISO/WD 19751-1, Office Equipment - Appearance-based image quality standards for printers - Part 1: Overview, Procedure and Common Methods, 2004 describes the overview of this multi-part appearance-based image quality standard. One of the ISO 19751 multi-part Standard"s tasks is to address the appearance-based gloss and gloss uniformity issues (in ISO 19751-2). This paper summarizes the current status and technical progress since the last two updates3, 4. In particular, we will be discussion our attempt to include 75 degree gloss (G75) objective measurement5 in differential gloss and within-page gloss uniformity. The result for a round-robin experiment involving objective measurement of differential gloss using G60 and G75 gloss measurement geometry is described. The results for two perceptual-based round-robin experiments relating to haze effect on the perception of gloss, and gloss artifacts (gloss streaks/bands, gloss graininess/mottle) are discussed.

Gloss uniformity measurement update for ISO/IEC 19751

NASA Astrophysics Data System (ADS)

Ng, Yee S.; Cui, Chengwu; Kuo, Chunghui; Maggard, Eric; Mashtare, Dale; Morris, Peter

2004-10-01

To address the standardization issues of perceptually based image quality for printing systems, ISO/IEC JTC1/SC28, the standardization committee for office equipment chartered the W1.1 project with the responsibility of drafting a proposal for an international standard for the evaluation of printed image quality1. An ISO draft Standard2, ISO/WD 19751-1, Office Equipment - Appearance-based image quality standards for printers - Part 1: Overview, Procedure and Common Methods, 2004 describes the overview of this multi-part appearance-based image quality standard. One of the ISO 19751 multi-part Standard"s tasks is to address the appearance-based gloss and gloss uniformity issues (in ISO 19751-2). This paper summarizes the current status and technical progress since the last two updates3, 4. In particular, we will be discussion our attempt to include 75 degree gloss (G75) objective measurement5 in differential gloss and within-page gloss uniformity. The result for a round-robin experiment involving objective measurement of differential gloss using G60 and G75 gloss measurement geometry is described. The results for two perceptual-based round-robin experiments relating to haze effect on the perception of gloss, and gloss artifacts (gloss streaks/bands, gloss graininess/mottle) are discussed.

The high-quality draft genome of peach (Prunus persica) identifies unique patterns of genetic diversity, domestication and genome evolution.

PubMed

Verde, Ignazio; Abbott, Albert G; Scalabrin, Simone; Jung, Sook; Shu, Shengqiang; Marroni, Fabio; Zhebentyayeva, Tatyana; Dettori, Maria Teresa; Grimwood, Jane; Cattonaro, Federica; Zuccolo, Andrea; Rossini, Laura; Jenkins, Jerry; Vendramin, Elisa; Meisel, Lee A; Decroocq, Veronique; Sosinski, Bryon; Prochnik, Simon; Mitros, Therese; Policriti, Alberto; Cipriani, Guido; Dondini, Luca; Ficklin, Stephen; Goodstein, David M; Xuan, Pengfei; Del Fabbro, Cristian; Aramini, Valeria; Copetti, Dario; Gonzalez, Susana; Horner, David S; Falchi, Rachele; Lucas, Susan; Mica, Erica; Maldonado, Jonathan; Lazzari, Barbara; Bielenberg, Douglas; Pirona, Raul; Miculan, Mara; Barakat, Abdelali; Testolin, Raffaele; Stella, Alessandra; Tartarini, Stefano; Tonutti, Pietro; Arús, Pere; Orellana, Ariel; Wells, Christina; Main, Dorrie; Vizzotto, Giannina; Silva, Herman; Salamini, Francesco; Schmutz, Jeremy; Morgante, Michele; Rokhsar, Daniel S

2013-05-01

Rosaceae is the most important fruit-producing clade, and its key commercially relevant genera (Fragaria, Rosa, Rubus and Prunus) show broadly diverse growth habits, fruit types and compact diploid genomes. Peach, a diploid Prunus species, is one of the best genetically characterized deciduous trees. Here we describe the high-quality genome sequence of peach obtained from a completely homozygous genotype. We obtained a complete chromosome-scale assembly using Sanger whole-genome shotgun methods. We predicted 27,852 protein-coding genes, as well as noncoding RNAs. We investigated the path of peach domestication through whole-genome resequencing of 14 Prunus accessions. The analyses suggest major genetic bottlenecks that have substantially shaped peach genome diversity. Furthermore, comparative analyses showed that peach has not undergone recent whole-genome duplication, and even though the ancestral triplicated blocks in peach are fragmentary compared to those in grape, all seven paleosets of paralogs from the putative paleoancestor are detectable.

Drafting.

ERIC Educational Resources Information Center

Hughes, Larry R.

This guide to teaching drafting, one in a series of instructional materials for junior high industrial arts education, is designed to assist teachers as they plan and implement new courses of study and as they make revisions and improvements in existing courses in order to integrate classroom learning with real-life experiences. This drafting…

Articulation Workshop in Drafting. Final Report.

ERIC Educational Resources Information Center

Catonsville Community Coll., MD.

Drafting students from vocational/technical and high schools frequently repeat the same experiences and courses and work with the same instructional materials when they enroll in college engineering drawing, and architecture programs. In order to minimize needless repetition and address the problem of articulation between such schools and the…

Integrated Computer-Aided Drafting Instruction (ICADI).

ERIC Educational Resources Information Center

Chen, C. Y.; McCampbell, David H.

Until recently, computer-aided drafting and design (CAD) systems were almost exclusively operated on mainframes or minicomputers and their cost prohibited many schools from offering CAD instruction. Today, many powerful personal computers are capable of performing the high-speed calculation and analysis required by the CAD application; however,…

Development of an Official Guideline for the Economic Evaluation of Drugs/Medical Devices in Japan.

PubMed

Shiroiwa, Takeru; Fukuda, Takashi; Ikeda, Shunya; Takura, Tomoyuki; Moriwaki, Kensuke

2017-03-01

In Japan, cost-effectiveness evaluation was implemented on a trial basis from fiscal year 2016. The results will be applied to the future repricing of drugs and medical devices. On the basis of a request from the Central Social Insurance Medical Council (Chuikyo), our research team drafted the official methodological guideline for trial implementation. Here, we report the process of developing and the contents of the official guideline for cost-effectiveness evaluation. The guideline reflects discussions at the Chuikyo subcommittee (e.g., the role of quality-adjusted life-year) and incorporates our academic perspective. Team members generated research questions for each section of the guideline and discussions on these questions were carried out. A draft guideline was prepared and submitted to the Ministry of Health, Labour and Welfare (MHLW), and then to the subcommittee. The draft guideline was revised on the basis of the discussions at the subcommitte, if appropriate. Although the "public health care payer's perspective" is standard in this guideline, other perspectives can be applied as necessary depending on the objective of analysis. On the basis of the discussions at the subcommittee, quality-adjusted life-year will be used as the basic outcome. A discount rate of 2% per annum for costs and outcomes is recommended. The final guideline was officially approved by the Chuikyo general assembly in February 2016. This is the first officially approved guideline for the economic evaluation of drugs and medical devices in Japan. The guideline is expected to improve the quality and comparability of submitted cost-effectiveness data for decision making. Copyright © 2017 International Society for Pharmacoeconomics and Outcomes Research (ISPOR). Published by Elsevier Inc. All rights reserved.

Draft Genome Sequence of Sporolactobacillus inulinus Strain CASD, an Efficient d-Lactic Acid-Producing Bacterium with High-Concentration Lactate Tolerance Capability

PubMed Central

Yu, Bo; Su, Fei; Wang, Limin; Xu, Ke; Zhao, Bo; Xu, Ping

2011-01-01

Sporolactobacillus inulinus CASD is an efficient d-lactic acid producer with high optical purity. Here we report for the first time the draft genome sequence of S. inulinus (2,930,096 bp). The large number of annotated two-component system genes makes it possible to explore the mechanism of extraordinary lactate tolerance of S. inulinus CASD. PMID:21952540

Draft genome sequence of Sporolactobacillus inulinus strain CASD, an efficient D-lactic acid-producing bacterium with high-concentration lactate tolerance capability.

PubMed

Yu, Bo; Su, Fei; Wang, Limin; Xu, Ke; Zhao, Bo; Xu, Ping

2011-10-01

Sporolactobacillus inulinus CASD is an efficient D-lactic acid producer with high optical purity. Here we report for the first time the draft genome sequence of S. inulinus (2,930,096 bp). The large number of annotated two-component system genes makes it possible to explore the mechanism of extraordinary lactate tolerance of S. inulinus CASD.

Flow characteristics and spillage mechanisms of an inclined quad-vortex range hood subject to influence from draft.

PubMed

Huang, Rong Fung; Chen, Jia-Kun; Lin, Jyun-Hua

2015-01-01

The flow and spillage characteristics of an inclined quad-vortex (IQV) range hood subject to the influence of drafts from various directions were studied. The laser-assisted smoke flow visualization technique was used to reveal the flow characteristics, and the tracer-gas (sulfur hexafluoride) concentration detection method was used to indicate the quantitative values of the capture efficiency of the hood. It was found that the leakage mechanisms of the IQV range hood are closely related to the flow characteristics. A critical draft velocity of about 0.5 m/s and a critical face velocity of about 0.25 m/s for the IQV range hood were found. When the IQV range hood was influenced by a draft with a velocity larger than the critical draft velocity, the spillage of pollutants became significant and the pollutant spillage rate increased with increasing draft velocity. At draft velocities less than or equal to the critical value, no containment leakages induced by the turbulence diffusion, reverse flow, or boundary-layer separation were observed, and the capture efficiency was about 100%. The IQV range hood exhibited a high ability to resist the influences of lateral and frontal drafts. The capture efficiency of the IQV range hood operated at the suction flow rate 5 to 9 m(3)/min is higher than that of the conventional range hood operated at 11 to 15 m(3)/min.

Vortex rope instabilities in a model of conical draft tube

NASA Astrophysics Data System (ADS)

Skripkin, Sergey; Tsoy, Mikhail; Kuibin, Pavel; Shtork, Sergey

2017-10-01

We report on experimental studies of the formation of vortex ropes in a laboratory simplified model of hydroturbine draft tube. Work is focused on the observation of various flow patterns at the different rotational speed of turbine runner at fixed flow rate. The measurements involve high-speed visualization and pressure pulsations recordings. Draft tube wall pressure pulsations are registered by pressure transducer for different flow regimes. Vortex rope precession frequency were calculated using FFT transform. The experiments showed interesting features of precessing vortex rope like twin spiral and formation of vortex ring.

Drafting with Design in Mind

ERIC Educational Resources Information Center

Daugherty, Michael K.; Carter, Vinson

2011-01-01

Design and drafting are subjects long taught in technology education, and subjects that retain high status in the profession. Admittedly, since the initial publication of Standards for Technological Literacy in 2000, design has taken on a larger role and meaning in the technology education profession. However, design continues to be delivered…

Draft Genome Sequence of the Algicidal Bacterium Mangrovimonas yunxiaonensis Strain LY01

PubMed Central

Li, Yi; Zhu, Hong; Li, Chongping; Zhang, Huajun; Chen, Zhangran; Zheng, Wei

2014-01-01

Mangrovimonas yunxiaonensis LY01, a novel bacterium isolated from mangrove sediment, showed high algicidal effects on harmful algal blooms of Alexandrium tamarense. Here, we present the first draft genome sequence of this strain to further understanding of the functional genes related to algicidal activity. PMID:25428978

Airbrush Technology.

ERIC Educational Resources Information Center

Micklus, C. Samuel

To supplement and extend the offerings in the fields of industrial arts and vocational education, instructions in the use of the airbrush in design oriented activities are presented in this manual. It is written for and directed to the following types of programs: (1) high school--drafting, graphic arts; (2) vocational school--drafting, commercial…

Review of the Draft K-12 Common Core Standards

ERIC Educational Resources Information Center

Carmichael, Sheila Byrd; Wilson, W. Stephen; Martino, Gabrielle; Finn, Chester E., Jr.; Porter-Magee, Kathleen; Winkler, Amber M.

2010-01-01

American education approached a possible turning point when the National Governors Association (NGA) and Council of Chief State School Officers (CCSSO) released drafts of proposed new academic standards in English language arts and math for kindergarten through high school. Already the object of much interest--and some controversy--these are…

76 FR 59155 - Notice of Availability of South Coast Draft Resource Management Plan Revision and Draft...

Federal Register 2010, 2011, 2012, 2013, 2014

2011-09-23

... . Persons who use a telecommunications device for the deaf (TDD) may call the Federal Information Relay... portions of five highly urbanized southern California counties: San Diego, Riverside, San Bernardino... Concern (ACEC), sensitive species and other wildlife habitat, lands with wilderness characteristics...

78 FR 40199 - Draft Spent Fuel Storage and Transportation Interim Staff Guidance

Federal Register 2010, 2011, 2012, 2013, 2014

2013-07-03

... NUCLEAR REGULATORY COMMISSION [NRC-2013-0140] Draft Spent Fuel Storage and Transportation Interim... Spent Fuel Storage and Transportation Interim Staff Guidance No. 24 (SFST-ISG-24), Revision 0, ``The Use of a Demonstration Program as Confirmation of Integrity for Continued Storage of High Burnup Fuel...

High quality draft genome sequence of Bacteroides barnesiae type strain BL2T (DSM 18169T) from chicken caecum

DOE Office of Scientific and Technical Information (OSTI.GOV)

Sakamoto, Mitsuo; Lapidus, Alla L.; Han, James

Bacteroides barnesiae Lan et al. 2006 is a species of the genus Bacteroides, which belongs to the family Bacteroidaceae. Strain BL2T is of interest because it was isolated from the gut of a chicken and the growing awareness that the anaerobic microbiota of the caecum is of benefit for the host and may impact poultry farming. We report that the 3,621,509 bp long genome with its 3,059 protein-coding and 97 RNA genes is a part of the Genomic Encyclopedia of Type Strains, Phase I: the one thousand microbial genomes (KMG) project.

High quality draft genome sequence of Bacteroides barnesiae type strain BL2T (DSM 18169T) from chicken caecum

DOE PAGES

Sakamoto, Mitsuo; Lapidus, Alla L.; Han, James; ...

2015-08-02

Bacteroides barnesiae Lan et al. 2006 is a species of the genus Bacteroides, which belongs to the family Bacteroidaceae. Strain BL2T is of interest because it was isolated from the gut of a chicken and the growing awareness that the anaerobic microbiota of the caecum is of benefit for the host and may impact poultry farming. We report that the 3,621,509 bp long genome with its 3,059 protein-coding and 97 RNA genes is a part of the Genomic Encyclopedia of Type Strains, Phase I: the one thousand microbial genomes (KMG) project.

40 CFR 1503.1 - Inviting comments.

Code of Federal Regulations, 2011 CFR

2011-07-01

... clearinghouse, for securing State and local reviews of the draft environmental impact statements. (3) Request... 40 Protection of Environment 33 2011-07-01 2011-07-01 false Inviting comments. 1503.1 Section 1503.1 Protection of Environment COUNCIL ON ENVIRONMENTAL QUALITY COMMENTING § 1503.1 Inviting comments...

40 CFR 1503.1 - Inviting comments.

Code of Federal Regulations, 2010 CFR

2010-07-01

... clearinghouse, for securing State and local reviews of the draft environmental impact statements. (3) Request... 40 Protection of Environment 32 2010-07-01 2010-07-01 false Inviting comments. 1503.1 Section 1503.1 Protection of Environment COUNCIL ON ENVIRONMENTAL QUALITY COMMENTING § 1503.1 Inviting comments...

Computer-Integrated Manufacturing Technology. Tech Prep Competency Profile.

ERIC Educational Resources Information Center

Lakeland Tech Prep Consortium, Kirtland, OH.

This tech prep competency profile for computer-integrated manufacturing technology begins with definitions for four occupations: manufacturing technician, quality technician, mechanical engineering technician, and computer-assisted design/drafting (CADD) technician. A chart lists competencies by unit and indicates whether entire or partial unit is…

77 FR 5247 - Draft Integrated Science Assessment for Lead

Federal Register 2010, 2011, 2012, 2013, 2014

2012-02-02

... to identify certain pollutants, which among other things, ``cause or contribute to air pollution... other things) with independent scientific review of EPA's air quality criteria. On February 26, 2010 (75..., are publicly available only in hard copy. Publicly available docket materials are available either...

EU-US ABWG AgENCODE Workshop

USDA-ARS?s Scientific Manuscript database

As considerable progress has been made on producing draft quality genomic sequence for many food animal species, the next goal for genomics research is a greater understanding of gene regulation and expression. The EU-US Animal Biotechnology Working Group (ABWG), established by the EU-US Biotechnolo...

How to Write Your First Research Paper

PubMed Central

Kallestinova, Elena D.

2011-01-01

Writing a research manuscript is an intimidating process for many novice writers in the sciences. One of the stumbling blocks is the beginning of the process and creating the first draft. This paper presents guidelines on how to initiate the writing process and draft each section of a research manuscript. The paper discusses seven rules that allow the writer to prepare a well-structured and comprehensive manuscript for a publication submission. In addition, the author lists different strategies for successful revision. Each of those strategies represents a step in the revision process and should help the writer improve the quality of the manuscript. The paper could be considered a brief manual for publication. PMID:21966034

How to write your first research paper.

PubMed

Kallestinova, Elena D

2011-09-01

Writing a research manuscript is an intimidating process for many novice writers in the sciences. One of the stumbling blocks is the beginning of the process and creating the first draft. This paper presents guidelines on how to initiate the writing process and draft each section of a research manuscript. The paper discusses seven rules that allow the writer to prepare a well-structured and comprehensive manuscript for a publication submission. In addition, the author lists different strategies for successful revision. Each of those strategies represents a step in the revision process and should help the writer improve the quality of the manuscript. The paper could be considered a brief manual for publication. Copyright © 2011.

Draft genome sequence of a multidrug-resistant Aeromonas hydrophila ST508 strain carrying rmtD and blaCTX-M-131 isolated from a bloodstream infection.

PubMed

Moura, Quézia; Fernandes, Miriam R; Cerdeira, Louise; Santos, Ana Carolina M; de Souza, Tiago A; Ienne, Susan; Pignatari, Antonio Carlos C; Gales, Ana C; Silva, Rosa M; Lincopan, Nilton

2017-09-01

Here we report the draft genome sequence of a multidrug-resistant (MDR) Aeromonas hydrophila strain belonging to sequence type 508 (ST508) isolated from a human bloodstream infection. Assembly and annotation of this draft genome resulted in 5028498bp and revealed the presence of 16S rRNA methylase rmtD and bla CTX-M-131 genes encoding high-level resistance to aminoglycosides and cephalosporins, respectively, as well as multiple virulence genes. This draft genome can provide significant information for understanding mechanisms on the establishment and treatment of infections caused by this pathogen. Copyright © 2017 International Society for Chemotherapy of Infection and Cancer. Published by Elsevier Ltd. All rights reserved.

Draft Genome Sequences of Klebsiella oxytoca Isolates Originating from a Highly Contaminated Liquid Hand Soap Product.

PubMed

Hammerl, J A; Lasch, P; Nitsche, A; Dabrowski, P W; Hahmann, H; Wicke, A; Kleta, S; Al Dahouk, S; Dieckmann, R

2015-07-23

In 2013, contaminated liquid soap was detected by routine microbiological monitoring of consumer products through state health authorities. Because of its high load of Klebsiella oxytoca, the liquid soap was notified via the European Union Rapid Alert System for Dangerous Non-Food Products (EU-RAPEX) and recalled. Here, we present two draft genome sequences and a summary of their general features. Copyright © 2015 Hammerl et al.

58th SOW Low-Dust Helicopter Landing Zone Final Environmental Assessment

DTIC Science & Technology

2012-11-01

Effects AQCR Air Quality Control Region BASH Bird/wildlife-Aircraft Strike Hazard CEQ Council on Environmental Quality CFR Code of Federal Regulations...force would continue to be applied to minimize risks to aircrews and the general population. No unacceptable hazards to military personnel, the public...and Final EA As a result of comments received on the Draft EA, Section 3.1.2, Global Climate Change, and Hazardous and Toxic Materials and Waste

The Draft Genome Sequence of a Novel High-Efficient Butanol-Producing Bacterium Clostridium Diolis Strain WST.

PubMed

Chen, Chaoyang; Sun, Chongran; Wu, Yi-Rui

2018-03-21

A wild-type solventogenic strain Clostridium diolis WST, isolated from mangrove sediments, was characterized to produce high amount of butanol and acetone with negligible level of ethanol and acids from glucose via a unique acetone-butanol (AB) fermentation pathway. Through the genomic sequencing, the assembled draft genome of strain WST is calculated to be 5.85 Mb with a GC content of 29.69% and contains 5263 genes that contribute to the annotation of 5049 protein-coding sequences. Within these annotated genes, the butanol dehydrogenase gene (bdh) was determined to be in a higher amount from strain WST compared to other Clostridial strains, which is positively related to its high-efficient production of butanol. Therefore, we present a draft genome sequence analysis of strain WST in this article that should facilitate to further understand the solventogenic mechanism of this special microorganism.

Draft framework for watershed-based trading

DOE Office of Scientific and Technical Information (OSTI.GOV)

NONE

1996-05-30

Effluent trading is an innovative way for water quality agencies and community stakeholders to develop common-sense, cost-effective solutions for water quality problems in their watersheds. Trading can allow communities to grow and prosper while retaining their commitment to water quality. The bulk of this framework discusses effluent trading in watersheds. Remaining sections discuss transactions that, while not technically fulfilling the definition of `effluent` trade, do involve the exchange of valued water quality or other ecological improvements between partners responding to market initiatives. This document therefore includes activities such as trades within a facility (intra-plant trading) and wetland mitigation banking, effluentmore » trading/watersheds/watershed management/water quality protection/water quality management.« less

Draft genome sequence of Halomonas lutea strain YIM 91125 T (DSM 23508 T) isolated from the alkaline Lake Ebinur in Northwest China

DOE Office of Scientific and Technical Information (OSTI.GOV)

Gao, Xiao-Yang; Zhi, Xiao-Yang; Li, Hong-Wei

Species of the genus Halomonas are halophilic and their flexible adaption to changes of salinity and temperature brings considerable potential biotechnology applications, such as degradation of organic pollutants and enzyme production. The type strain Halomonas lutea YIM 91125 T was isolated from a hypersaline lake in China. The genome of strain YIM 91125 T becomes the twelfth species sequenced in Halomonas, and the thirteenth species sequenced in Halomonadaceae. We described the features of H. lutea YIM 91125 T, together with the high quality draft genome sequence and annotation of its type strain. The 4,533,090 bp long genome of strain YIMmore » 91125 T with its 4,284 protein-coding and 84 RNA genes is a part of Genomic Encyclopedia of Type Strains, Phase I: the one thousand microbial genomes (KMG-I) project. From the viewpoint of comparative genomics, H. lutea has a larger genome size and more specific genes, which indicated acquisition of function bringing better adaption to its environment. Finally, DDH analysis demonstrated that H. lutea is a distinctive species, and halophilic features and nitrogen metabolism related genes were discovered in its genome.« less

Draft genome sequence of Halomonas lutea strain YIM 91125 T (DSM 23508 T) isolated from the alkaline Lake Ebinur in Northwest China

DOE PAGES

Gao, Xiao-Yang; Zhi, Xiao-Yang; Li, Hong-Wei; ...

2015-01-20

Species of the genus Halomonas are halophilic and their flexible adaption to changes of salinity and temperature brings considerable potential biotechnology applications, such as degradation of organic pollutants and enzyme production. The type strain Halomonas lutea YIM 91125 T was isolated from a hypersaline lake in China. The genome of strain YIM 91125 T becomes the twelfth species sequenced in Halomonas, and the thirteenth species sequenced in Halomonadaceae. We described the features of H. lutea YIM 91125 T, together with the high quality draft genome sequence and annotation of its type strain. The 4,533,090 bp long genome of strain YIMmore » 91125 T with its 4,284 protein-coding and 84 RNA genes is a part of Genomic Encyclopedia of Type Strains, Phase I: the one thousand microbial genomes (KMG-I) project. From the viewpoint of comparative genomics, H. lutea has a larger genome size and more specific genes, which indicated acquisition of function bringing better adaption to its environment. Finally, DDH analysis demonstrated that H. lutea is a distinctive species, and halophilic features and nitrogen metabolism related genes were discovered in its genome.« less

76 FR 5156 - Environmental Impact Statements; Notice of Availability

Federal Register 2010, 2011, 2012, 2013, 2014

2011-01-28

... Firm Water Supplies, Improve Water Quality, and to Reduce Water Costs, San Bernardino and Riverside.... 20110020, Draft EIS, NRCS, IA, Clarke County Water Supply, To Construct a Multiple-purpose Structure that Provides for Rural Water Supply and Water Based Recreational Opportunities, Clarke County, IA, Comment...

42 CFR 431.428 - Reporting requirements.

Code of Federal Regulations, 2012 CFR

2012-10-01

... providing insurance coverage to beneficiaries and uninsured populations. (4) Outcomes of care, quality of... performance of the demonstration. (8) The status of the evaluation and information regarding progress in... must publish its draft annual report on its public Web site within 30 days of submission to CMS. (1...

42 CFR 431.428 - Reporting requirements.

Code of Federal Regulations, 2013 CFR

2013-10-01

... providing insurance coverage to beneficiaries and uninsured populations. (4) Outcomes of care, quality of... performance of the demonstration. (8) The status of the evaluation and information regarding progress in... must publish its draft annual report on its public Web site within 30 days of submission to CMS. (1...

42 CFR 431.428 - Reporting requirements.

Code of Federal Regulations, 2014 CFR

2014-10-01

... providing insurance coverage to beneficiaries and uninsured populations. (4) Outcomes of care, quality of... performance of the demonstration. (8) The status of the evaluation and information regarding progress in... must publish its draft annual report on its public Web site within 30 days of submission to CMS. (1...

77 FR 8884 - Draft Guidance for Industry on Quality Considerations in Demonstrating Biosimilarity to a...

Federal Register 2010, 2011, 2012, 2013, 2014

2012-02-15

... information (e.g., characterization, adventitious agent safety, process controls, and specifications) for the... Manufacturing Process Assessment of Physiochemical Properties Functional Activities Receptor Binding and... 351(k) application. FDA will also seek OMB approval for this information collection. In addition, this...

High-quality permanent draft genome sequence of the extremely osmotolerant diphenol degrading bacterium Halotalea alkalilenta AW-7T, and emended description of the genus Halotalea

DOE PAGES

Ntougias, Spyridon; Lapidus, Alla; Copeland, Alex; ...

2015-08-13

Members of the genus Halotalea (family Halomonadaceae) are of high significance since they can tolerate the greatest glucose and maltose concentrations ever reported for known bacteria and are involved in the degradation of industrial effluents. Here, the characteristics and the permanent-draft genome sequence and annotation of Halotalea alkalilenta AW-7T are described. The microorganism was sequenced as a part of the Genomic Encyclopedia of Type Strains, Phase I: the one thousand microbial genomes (KMG) project at the DOE Joint Genome Institute, and it is the only strain within the genus Halotalea having its genome sequenced. The genome is 4,467,826 bp longmore » and consists of 40 scaffolds with 64.62 % average GC content. A total of 4,104 genes were predicted, comprising of 4,028 protein-coding and 76 RNA genes. Most protein-coding genes (87.79 %) were assigned to a putative function. Halotalea alkalilenta AW-7T encodes the catechol and protocatechuate degradation to β-ketoadipate via the β-ketoadipate and protocatechuate ortho-cleavage degradation pathway, and it possesses the genetic ability to detoxify fluoroacetate, cyanate and acrylonitrile. Lastly, an emended description of the genus Halotalea Ntougias et al. 2007 is also provided in order to describe the delayed fermentation ability of the type strain.« less

Draft Genome Sequence of Mycobacterium chimaera Type Strain Fl-0169

EPA Science Inventory

We report the draft genome sequence of the type strain Mycobacterium chimaera Fl-0169T, a member of the Mycobacterium avium complex (MAC). M. chimaera Fl-0169T was isolated from a patient in Italy and is highly similar to strains of M. chimaera isolated in Ireland, though Fl-016...

Draft Genome Sequence of Streptomyces specialis Type Strain GW41-1564 (DSM 41924).

PubMed

Loucif, Lotfi; Michelle, Caroline; Terras, Jérôme; Rolain, Jean-Marc; Raoult, Didier; Fournier, Pierre-Edouard

2017-03-30

Here, we report the draft genome sequence of Streptomyces specialis type strain GW41-1564, which was isolated from soil. This 5.87-Mb genome exhibits a high G+C content of 72.72% and contains 5,486 protein-coding genes. Copyright © 2017 Loucif et al.

Draft Genome Sequence of the Algicidal Bacterium Mangrovimonas yunxiaonensis Strain LY01.

PubMed

Li, Yi; Zhu, Hong; Li, Chongping; Zhang, Huajun; Chen, Zhangran; Zheng, Wei; Xu, Hong; Zheng, Tianling

2014-11-26

Mangrovimonas yunxiaonensis LY01, a novel bacterium isolated from mangrove sediment, showed high algicidal effects on harmful algal blooms of Alexandrium tamarense. Here, we present the first draft genome sequence of this strain to further understanding of the functional genes related to algicidal activity. Copyright © 2014 Li et al.

78 FR 68859 - Draft Environmental Impact Statement for the Proposed Fee-to-Trust Transfer of Property and...

Federal Register 2010, 2011, 2012, 2013, 2014

2013-11-15

..., intends to file a draft environmental impact statement (DEIS) with the U.S. Environmental Protection... Environmental Protection Agency publishes its Notice of Availability in the Federal Register. ADDRESSES: You may... hearings will be held at the Taunton High School, 50 William Street, Taunton, Massachusetts, and Mashpee...

Computer Aided Design: Instructional Manual. The North Dakota High Technology Mobile Laboratory Project.

ERIC Educational Resources Information Center

Cheng, Wan-Lee

This instructional manual contains 12 learning activity packets for use in a workshop in computer-aided design and drafting (CADD). The lessons cover the following topics: introduction to computer graphics and computer-aided design/drafting; coordinate systems; advance space graphics hardware configuration and basic features of the IBM PC…

26 CFR 48.4042-3 - Certain types of commercial waterway transportation excluded.

Code of Federal Regulations, 2010 CFR

2010-04-01

... Inland Waterways § 48.4042-3 Certain types of commercial waterway transportation excluded. (a) Deep draft... waters other than the territorial waters of the United States or any other country. Thus, the high seas... measured from the deepest appendage. A separage determination of draft must be made for each voyage when...

SSTAC/ARTS review of the draft Integrated Technology Plan (ITP). Volume 8: Aerothermodynamics Automation and Robotics (A/R) systems sensors, high-temperature superconductivity

NASA Technical Reports Server (NTRS)

1991-01-01

Viewgraphs of briefings presented at the SSTAC/ARTS review of the draft Integrated Technology Plan (ITP) on aerothermodynamics, automation and robotics systems, sensors, and high-temperature superconductivity are included. Topics covered include: aerothermodynamics; aerobraking; aeroassist flight experiment; entry technology for probes and penetrators; automation and robotics; artificial intelligence; NASA telerobotics program; planetary rover program; science sensor technology; direct detector; submillimeter sensors; laser sensors; passive microwave sensing; active microwave sensing; sensor electronics; sensor optics; coolers and cryogenics; and high temperature superconductivity.

SSTAC/ARTS review of the draft Integrated Technology Plan (ITP). Volume 8: Aerothermodynamics Automation and Robotics (A/R) systems sensors, high-temperature superconductivity

DOE Office of Scientific and Technical Information (OSTI.GOV)

Not Available

Viewgraphs of briefings presented at the SSTAC/ARTS review of the draft Integrated Technology Plan (ITP) on aerothermodynamics, automation and robotics systems, sensors, and high-temperature superconductivity are included. Topics covered include: aerothermodynamics; aerobraking; aeroassist flight experiment; entry technology for probes and penetrators; automation and robotics; artificial intelligence; NASA telerobotics program; planetary rover program; science sensor technology; direct detector; submillimeter sensors; laser sensors; passive microwave sensing; active microwave sensing; sensor electronics; sensor optics; coolers and cryogenics; and high temperature superconductivity.

Use of a Pediatric Cardiovascular Nursing Consortium for Development and Evaluation of Quality Measures: The C4-MNP Experience.

PubMed

Connor, Jean A; Larson, Carol; Baird, Jennifer; Hickey, Patricia A

2016-01-01

The evidence linking nursing care and patient outcomes has been globally demonstrated. Thus, it is time for translation and application of this evidence to robust measurement that uniquely demonstrates the value of nursing care and the characteristics of the nursing workforce that contribute to optimal patient outcomes. The aim of this study was to identify and develop standardized measures representative of pediatric nursing care of the cardiovascular patient for benchmarking within freestanding children's hospitals. Using a consensus-based approach, the Consortium of Congenital Cardiac Care- Measurement of Nursing Practice (C4-MNP) members developed quality measures within working groups and then individually critiqued all drafted measures. Final draft measures were then independently reviewed and critiqued by an external nursing quality measurement committee. The final quality measures were also made available to a national parent support group for feedback. The development process used by C4-MNP resulted in 10 measures eligible for testing across freestanding children's hospitals. Employing a collaborative consensus-based method plus implementing the criteria of the National Quality Forum and external vetting period provided a strong framework for the development and evaluation of standardized measures. The Consortium will continue with implementation and testing of each measure in 9 of our 28 collaborating centers. This activity will support initial development of benchmarks and evaluation of the association of the measures with patient outcomes. Copyright © 2016 Elsevier Inc. All rights reserved.

National Football League Skilled and Unskilled Positions Vary in Opportunity and Yield in Return to Play After an Anterior Cruciate Ligament Injury

PubMed Central

Yang, JaeWon; Hodax, Jonathan D.; Machan, Jason T.; Secrist, Eric S.; Durand, Wesley M.; Owens, Brett D.; Eltorai, Adam E.M.; Dodson, Christopher C.

2017-01-01

Background: Anterior cruciate ligament (ACL) injuries pose a significant risk to the careers of players in the National Football League (NFL). The relationships between draft round and position on return to play (RTP) among NFL players are not well understood, and the ability to return to preinjury performance levels remains unknown for most positions. Purpose: To test for differences in RTP rates and changes in performance after an ACL injury by position and draft round. We hypothesized that skilled positions would return at a lower rate compared to unskilled positions. We further hypothesized that early draft-round status would relate to a greater rate of RTP and that skilled positions and a lower draft round would correlate with decreased performance for players who return to sport. Study Design: Case-control study; Level of evidence, 3. Methods: Utilizing a previously established database of publicly available information regarding ACL tears among NFL players, athletes with ACL tears occurring between the 2010 and 2013 seasons were identified. Generalized linear models and Kaplan-Meier time-to-event models were used to test the study hypotheses. Results: The overall RTP rate was 61.7%, with skilled players and unskilled players returning at rates of 64.1% and 60.4%, respectively (P = .74). Early draft-round players and unskilled late draft-round players had greater rates of RTP compared to skilled late draft-round players and both unskilled and skilled undrafted free agents (UDFAs). Skilled early draft-round players constituted the only cohort that played significantly fewer games after an injury. Unskilled UDFAs constituted the only cohort to show a significant increase in the number of games started and ratio of games started to games played, starting more games in which they played, after an injury. Conclusion: Early draft-round and unskilled players were more likely to return compared to their later draft-round and skilled peers. Skilled early draft-round players, who displayed relatively high rates of RTP, constituted the only cohort to show a decline in performance. Unskilled UDFAs, who exhibited relatively low rates of RTP, constituted the only cohort to show an increase in performance. The significant effect of draft round and position type on RTP may be caused by a combination of differences in talent levels and in opportunities given to returning to play. PMID:28975136

Householder's Perceptions of Insulation Adequacy and Drafts in the Home in 2001

EIA Publications

2004-01-01

In order to improve the estimation of end-use heating consumption, the Energy Information Administration's (EIA), 2001 Residential Energy Consumption Survey (RECS), for the first time, asked respondents to judge how drafty they perceived their homes to be as a measure of insulation quality.

Emerging Media in Engineering Technology: A Case Study in Higher Education

ERIC Educational Resources Information Center

Ostler, Karl B.

2013-01-01

Technical illustration, 3D modeling, and design drafting under the discipline of engineering technology face rapid change and advancements in technologies such that educational leaders must continually anticipate change and make intelligent choices in providing a quality educational experience to adult students. However, how successful program…

78 FR 67140 - Office of Environmental Information; Pause the Development of the Draft Quality Standard for...

Federal Register 2010, 2011, 2012, 2013, 2014

2013-11-08

... ENVIRONMENTAL PROTECTION AGENCY [FRL-9902-70-OEI] Office of Environmental Information; Pause the... (External) Organizations and Two Associated QA Handbooks AGENCY: Environmental Protection Agency (EPA... Environmental Data Collection, Production, and Use by Non-EPA (External) Organizations and two associated QA...

76 FR 41192 - Mines Management Inc. Montanore Project, Kootenai National Forest, Lincoln County, MT

Federal Register 2010, 2011, 2012, 2013, 2014

2011-07-13

... DEPARTMENT OF AGRICULTURE Forest Service Mines Management Inc. Montanore Project, Kootenai... can be reviewed at: http://www.fs.fed.usda.gov/goto/kootenai/projects . Mines Management Inc. owns two... of Environmental Quality, issued a Draft Environmental Impact Statement for the Montanore Project. In...

21 CFR 211.160 - General requirements.

Code of Federal Regulations, 2011 CFR

2011-04-01

... 21 Food and Drugs 4 2011-04-01 2011-04-01 false General requirements. 211.160 Section 211.160 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN SERVICES (CONTINUED) DRUGS... drafted by the appropriate organizational unit and reviewed and approved by the quality control unit. The...

78 FR 70040 - Draft Integrated Science Assessment for Nitrogen Oxides-Health Criteria

Federal Register 2010, 2011, 2012, 2013, 2014

2013-11-22

... (NO 2 ). The Integrated Science Assessment (ISA), in conjunction with additional technical and policy... the secondary (welfare-based) NAAQS for NO 2 , in conjunction with a review of the secondary NAAQS for... called an Air Quality Criteria Document). The ISA, in conjunction with additional technical and policy...

75 FR 16837 - Public Review of Draft United States Thoroughfare, Landmark, and Postal Address Data Standard

Federal Register 2010, 2011, 2012, 2013, 2014

2010-04-02

... the structure of their address information but have not defined the elements that constitute an address. Knowledge of structure, content, and quality is required to successfully share information in a... discrete elements of address information and provides standardized terminology and definitions to alleviate...

77 FR 46952 - Approval and Promulgation of Air Quality Implementation Plans; Wisconsin; Regional Haze

Federal Register 2010, 2011, 2012, 2013, 2014

2012-08-07

... ``its Draft Economic Incentives Program Guidance is relevant to this rulemaking.'' Sierra Club states... Economic Incentive Program Guidance and in program-specific guidance that more directly addresses specific... Economic Incentives Program Guidance. Comment: Sierra Club comments that, ``the emission limits proposed by...

77 FR 77079 - Agency Information Collection Activities: Proposed Collection: Comment Request

Federal Register 2010, 2011, 2012, 2013, 2014

2012-12-31

... the data collection plans and draft instruments, email [email protected] or call the HRSA Reports... accuracy of the estimated burden, (3) ways to enhance the quality, utility, and clarity of the information... Rural Hospital Flexibility Grant Program Performance Measure Determination (OMB No. 0915-xxxx) - New...

Measuring the Impact of Port of Charleston Activities on Local Air Quality

EPA Science Inventory

Ports are a critical feature of the nation’s economy; port commerce supports 13 million jobs and contributes $3.15 trillion to the economy. The value of goods shipped through seaports represents 11% of the GDP. The US has 360 commercial ports, including 150 deep-draft seaports....

76 FR 38650 - Draft Integrated Science Assessment for Lead

Federal Register 2010, 2011, 2012, 2013, 2014

2011-07-01

... Science Assessment for Lead AGENCY: Environmental Protection Agency. ACTION: Notice of extension of public... Lead'' (EPA/600/R-10/075A). The original Federal Register notice announcing the public comment period... review of the National Ambient Air Quality Standards (NAAQS) for Lead. DATES: The public comment period...

76 FR 10893 - Draft Integrated Science Assessment for Ozone and Related Photochemical Oxidants

Federal Register 2010, 2011, 2012, 2013, 2014

2011-02-28

... Oxidants'' will be available primarily via the Internet on the National Center for Environmental Assessment... Administrator to identify certain pollutants which, among other things, ``cause or contribute to air pollution... Clean Air Act, is charged (among other things) with independent scientific review of EPA's air quality...

Fact Sheets on Review of National Ambient Air Quality Standards for Ozone Staff Papers

EPA Pesticide Factsheets

The second draft Staff Paper points to an expanded body of health effects evidence suggesting a wide range of adverse health effects associated with exposure to ambient ozone. This is part of the process for review of the NAAQS for ground-level ozone.

Air Quality Criteria for Ozone and Other Photochemical Oxidants. External Review Draft No 1. Volume III

EPA Science Inventory

This review of the effects of photochemical oxidants on vegetation and the responses of vegetation to photochemical oxidants first discusses the general methodologies used in studies of air pollution effects to provide a basis for understanding the methods, approaches, and experi...

Turf and Lawn Management: A Course in Agricultural Education. Curriculum Guide. Preliminary Draft.

ERIC Educational Resources Information Center

Mercer, R. J., Ed.

The curriculum guide (preliminary draft) is a result of the revision of the total South Carolina agricultural education curriculum; the scope of the turf and lawn management industry and its direct and indirect employment opportunities provide ample reasons for such a course offering in South Carolina high schools. The guide presents objectives,…

Draft Genome Sequences of Three Escherichia coli Strains with Different In Vivo Pathogenicities in an Avian (Ascending) Infection Model of the Oviduct

PubMed Central

Thøfner, Ida Cecilie Naundrup; Pors, Susanne Elisabeth; Christensen, Henrik; Bisgaard, Magne; Christensen, Jens Peter

2015-01-01

Here, we present three draft genome sequences of Escherichia coli strains that experimentally were proven to possess low (strain D2-2), intermediate (Chronic_salp), or high virulence (Cp6salp3) in an avian (ascending) infection model of the oviduct. PMID:25953185

The UK's Draft Guidance for Health in SEA in light of HIA community priorities and the UNECE SEA Protocol

DOE Office of Scientific and Technical Information (OSTI.GOV)

Posas, Paula J., E-mail: pjposas@gmail.co

2011-04-15

The Protocol on Strategic Environmental Assessment (SEA) to the United Nations Economic Commission for Europe (UNECE) Espoo Convention came into force on 11 July 2010. This Protocol, to which the European Union is party, gives a legal basis for enhanced attention to human health in the SEA process. In this context, the United Kingdom's (UK's) 2007 Draft Guidance on Health in Strategic Environmental Assessment represents an important early government-led effort to bring health issues and public health considerations more significantly into the SEA process. Since the UK is a worldwide leader in environmental and various other types of impact assessment,more » and since other countries may eventually consider its example in efforts to meet UNECE SEA Protocol requirements, scrutiny of its outputs is warranted. This paper thus examines the UK's Draft Guidance from both HIA academic and practitioner perspectives. First it assesses the extent to which the Draft Guidance reflects recent issues and lessons learned in the academic literature. In order to make the assessment, a meta-analysis of 70 HIA-related peer-reviewed articles was undertaken to extract authors' priority recommendations. These recommendations were subsequently compared with the contents of the Draft Guidance. Secondly, the Draft Guidance was assessed for its accordance with recommendations of the UNECE SEA Protocol background paper written by two HIA practitioners. Overall, the Draft Guidance's accordance with both sets of recommendations was found to be high, with only a few easily-remedied gaps. This evaluation suggests that the UK's Draft Guidance can be a useful starting point in the creation of future guidance on health in SEA in both the UK and other countries.« less

Development of genomic tools in a widespread tropical tree, Symphonia globulifera L.f.: a new low-coverage draft genome, SNP and SSR markers.

PubMed

Olsson, Sanna; Seoane-Zonjic, Pedro; Bautista, Rocío; Claros, M Gonzalo; González-Martínez, Santiago C; Scotti, Ivan; Scotti-Saintagne, Caroline; Hardy, Olivier J; Heuertz, Myriam

2017-07-01

Population genetic studies in tropical plants are often challenging because of limited information on taxonomy, phylogenetic relationships and distribution ranges, scarce genomic information and logistic challenges in sampling. We describe a strategy to develop robust and widely applicable genetic markers based on a modest development of genomic resources in the ancient tropical tree species Symphonia globulifera L.f. (Clusiaceae), a keystone species in African and Neotropical rainforests. We provide the first low-coverage (11X) fragmented draft genome sequenced on an individual from Cameroon, covering 1.027 Gbp or 67.5% of the estimated genome size. Annotation of 565 scaffolds (7.57 Mbp) resulted in the prediction of 1046 putative genes (231 of them containing a complete open reading frame) and 1523 exact simple sequence repeats (SSRs, microsatellites). Aligning a published transcriptome of a French Guiana population against this draft genome produced 923 high-quality single nucleotide polymorphisms. We also preselected genic SSRs in silico that were conserved and polymorphic across a wide geographical range, thus reducing marker development tests on rare DNA samples. Of 23 SSRs tested, 19 amplified and 18 were successfully genotyped in four S. globulifera populations from South America (Brazil and French Guiana) and Africa (Cameroon and São Tomé island, F ST  = 0.34). Most loci showed only population-specific deviations from Hardy-Weinberg proportions, pointing to local population effects (e.g. null alleles). The described genomic resources are valuable for evolutionary studies in Symphonia and for comparative studies in plants. The methods are especially interesting for widespread tropical or endangered taxa with limited DNA availability. © 2016 John Wiley & Sons Ltd.

Scenario drafting to anticipate future developments in technology assessment.

PubMed

Retèl, Valesca P; Joore, Manuela A; Linn, Sabine C; Rutgers, Emiel J T; van Harten, Wim H

2012-08-16

Health Technology Assessment (HTA) information, and in particular cost-effectiveness data is needed to guide decisions, preferably already in early stages of technological development. However, at that moment there is usually a high degree of uncertainty, because evidence is limited and different development paths are still possible. We developed a multi-parameter framework to assess dynamic aspects of a technology -still in development-, by means of scenario drafting to determine the effects, costs and cost-effectiveness of possible future diffusion patterns. Secondly, we explored the value of this method on the case of the clinical implementation of the 70-gene signature for breast cancer, a gene expression profile for selecting patients who will benefit most from chemotherapy. To incorporate process-uncertainty, ten possible scenarios regarding the introduction of the 70-gene signature were drafted with European experts. Out of 5 most likely scenarios, 3 drivers of diffusion (non-compliance, technical failure, and uptake) were quantitatively integrated in a decision-analytical model. For these scenarios, the cost-effectiveness of the 70-gene signature expressed in Incremental Cost-Effectiveness Ratios (ICERs) was compared to clinical guidelines, calculated from the past (2005) until the future (2020). In 2005 the ICER was €1,9 million/quality-adjusted-life-year (QALY), meaning that the 70-gene signature was not yet cost-effective compared to the current clinical guideline. The ICER for the 70-gene signature improved over time with a range of €1,9 million to €26,145 in 2010 and €1,9 million to €11,123/QALY in 2020 depending on the separate scenario used. From 2010, the 70-gene signature should be cost-effective, based on the combined scenario. The uptake-scenario had strongest influence on the cost-effectiveness. When optimal diffusion of a technology is sought, incorporating process-uncertainty by means of scenario drafting into a decision model may reveal unanticipated developments and can demonstrate a range of possible cost-effectiveness outcomes. The effect of scenarios give additional information on the speed with cost effectiveness might be reached and thus provide a more realistic picture for policy makers, opinion leaders and manufacturers.

Scenario drafting to anticipate future developments in technology assessment

PubMed Central

2012-01-01

Background Health Technology Assessment (HTA) information, and in particular cost-effectiveness data is needed to guide decisions, preferably already in early stages of technological development. However, at that moment there is usually a high degree of uncertainty, because evidence is limited and different development paths are still possible. We developed a multi-parameter framework to assess dynamic aspects of a technology -still in development-, by means of scenario drafting to determine the effects, costs and cost-effectiveness of possible future diffusion patterns. Secondly, we explored the value of this method on the case of the clinical implementation of the 70-gene signature for breast cancer, a gene expression profile for selecting patients who will benefit most from chemotherapy. Methods To incorporate process-uncertainty, ten possible scenarios regarding the introduction of the 70-gene signature were drafted with European experts. Out of 5 most likely scenarios, 3 drivers of diffusion (non-compliance, technical failure, and uptake) were quantitatively integrated in a decision-analytical model. For these scenarios, the cost-effectiveness of the 70-gene signature expressed in Incremental Cost-Effectiveness Ratios (ICERs) was compared to clinical guidelines, calculated from the past (2005) until the future (2020). Results In 2005 the ICER was €1,9 million/quality-adjusted-life-year (QALY), meaning that the 70-gene signature was not yet cost-effective compared to the current clinical guideline. The ICER for the 70-gene signature improved over time with a range of €1,9 million to €26,145 in 2010 and €1,9 million to €11,123/QALY in 2020 depending on the separate scenario used. From 2010, the 70-gene signature should be cost-effective, based on the combined scenario. The uptake-scenario had strongest influence on the cost-effectiveness. Conclusions When optimal diffusion of a technology is sought, incorporating process-uncertainty by means of scenario drafting into a decision model may reveal unanticipated developments and can demonstrate a range of possible cost-effectiveness outcomes. The effect of scenarios give additional information on the speed with cost effectiveness might be reached and thus provide a more realistic picture for policy makers, opinion leaders and manufacturers. PMID:22894140

Assessment of the Impacts of Global Change on Regional U.S. Air Quality (External Review Draft)

EPA Science Inventory

This report was prepared by the Global Change Research Program (GCRP) in the National Center for Environmental Assessment (NCEA) of the Office of Research and Development (ORD) at the U.S. Environmental Protection Agency (EPA). It is intended for managers and scientists worki...

Assessment of the Potential Impacts of Hydraulic Fracturing for Oil and Gas on Drinking Water Resources (External Review Draft)

EPA Science Inventory

This assessment provides a review and synthesis of available scientific literature and data to assess the potential for hydraulic fracturing for oil and gas to impact the quality or quantity of drinking water resources, and identifies factors affecting the frequency or severity o...

76 FR 16737 - Notice of Availability of the Draft Environmental Impact Statement for the Proposed South Coast...

Federal Register 2010, 2011, 2012, 2013, 2014

2011-03-25

..., visual effects, noise, vibration, cultural resources, air quality, open space, farmland, hazardous... is also available to review at the following locations: 1. State Transportation Library of Massachusetts 10 Park Plaza, 2nd Floor, Boston, MA. 2. Russell Memorial Library, 88 Main Street, Acushnet, MA. 3...

A Field-Based Aquatic Life Benchmark for Conductivity in Central Appalachian Streams (2010) (External Review Draft)

EPA Science Inventory

This report adapts the standard U.S. EPA methodology for deriving ambient water quality criteria. Rather than use toxicity test results, the adaptation uses field data to determine the loss of 5% of genera from streams. The method is applied to derive effect benchmarks for disso...

Proper Installation of Replacement Windows | Efficient Windows

Science.gov Websites

. Quality installation is critical for an airtight fit and a continuous water barrier to prevent drafts , water damage and condensation. The complexity of the installation depends on whether you are considering and specifications. Install level, plumb, and square. Install water tight: water must be prevented

National Policy on Education and Higher Education

ERIC Educational Resources Information Center

Varughese, Rajan

2017-01-01

The Ministry of Human Resource Development (MHRD) has come out with the draft of 'National Education Policy (NEP) 2016' in April 2016. The new NEP 2016 seeks to create conditions to improve the quality of teaching, learning and assessment, and promote transparency in the management of education in the country. The policy prescriptions enunciated…

Educating for Sustainability in Language Degrees: A Tale of 2 Case-Studies

ERIC Educational Resources Information Center

Hubscher-Davidson, Séverine; Panichelli-Batalla, Stéphanie

2016-01-01

Purpose: According to the 2014 joint report drafted by the Quality Assurance Agency and Higher Education Academy (QAA/HEA), universities have a key role to play in the development of sustainability literate graduates who can contribute to an environmentally responsible society. This paper aims to assess language students' understanding of…

Design and quality standards for custom hybrid microcircuits

NASA Technical Reports Server (NTRS)

Allen, R. V.

1977-01-01

A hybrid microcircuit standard was developed after a thorough review of applicable NASA, military, industry, and technical society specifications and standards and compilation of comments from technical reviewers throughout the hybrid industry. The draft of the standard submitted to the technical reviewers, the comments from the reviewers, and the completed standard are discussed.

77 FR 38377 - Notice of Availability of the Draft Environmental Impact Statement: Los Angeles County, CA

Federal Register 2010, 2011, 2012, 2013, 2014

2012-06-27

... modernize the design at the I-405, SR-91 and a portion of the I-5 interchanges, modernize and reconfigure... Intelligent Transportation Systems (ITS) improvements; improvements to 42 local arterial intersections within the I-710 Corridor; aesthetic enhancements; and, drainage and water quality improvement design...

75 FR 63447 - Intent To Prepare a Draft Environmental Impact Statement for the Louisiana Coastal Area...

Federal Register 2010, 2011, 2012, 2013, 2014

2010-10-15

... quality of the environmental decision making. The public includes affected and interested Federal, state... EIS for the LCA Ecosystem Restoration Study, November 2004. The record of decision for the... Ecosystem Restoration Study, November 2004. The record of decision for the programmatic EIS was signed on...

78 FR 4165 - Notice of Availability of the Draft Environmental Impact Statement for the Arturo Mine Project...

Federal Register 2010, 2011, 2012, 2013, 2014

2013-01-18

... office, substation and associated power transmission lines, water wells, water distribution and sewer...; access; discharges to surface water; air quality (including mercury); and impacts to stream drainages... which may impact water resources, and the Elko County Board of Commissioners have raised concerns about...

75 FR 26839 - Metrics and Standards for Intercity Passenger Rail Service under Section 207 of the Passenger...

Federal Register 2010, 2011, 2012, 2013, 2014

2010-05-12

... performance and service quality of intercity passenger train operations. In compliance with the statute, the FRA and Amtrak jointly drafted performance metrics and standards for intercity passenger rail service... and Standards for Intercity Passenger Rail Service under Section 207 of the Passenger Rail Investment...

78 FR 29392 - Embedded Digital Devices in Safety-Related Systems, Systems Important to Safety, and Items Relied...

Federal Register 2010, 2011, 2012, 2013, 2014

2013-05-20

... NUCLEAR REGULATORY COMMISSION [NRC-2013-0098] Embedded Digital Devices in Safety-Related Systems... (NRC) is issuing for public comment Draft Regulatory Issue Summary (RIS) 2013-XX, ``Embedded Digital... requirements for the quality and reliability of basic components with embedded digital devices. DATES: Submit...

IFSM-predicted environmental and economic affects of bmps on representative farms in southeastern Pennsylvania

USDA-ARS?s Scientific Manuscript database

According to the U.S. EPA, agricultural runoff is the largest contributor to water quality degradation in the Chesapeake Bay, accounting for about 44 percent of nutrient pollution and 65 percent of sediment pollution that enters the Bay (Draft Chesapeake Bay TMDL, 2010). Pennsylvania produces the se...

77 FR 36534 - Third External Review Draft Integrated Science Assessment for Ozone and Related Photochemical...

Federal Register 2010, 2011, 2012, 2013, 2014

2012-06-19

... the Internet on the National Center for Environmental Assessment's home page under the Recent... which, among other things, ``cause or contribute to air pollution which may reasonably be anticipated to... Clean Air Act, is charged (among other things) with independent scientific review of EPA's air quality...

78 FR 51719 - Draft Toxicological Review of Benzo[a]pyrene: In Support of the Summary Information in the...

Federal Register 2010, 2011, 2012, 2013, 2014

2013-08-21

... Environmental Assessment (NCEA) within the EPA Office of Research and Development (ORD). EPA is releasing this... Program is a human health assessment program that evaluates quantitative and qualitative risk information... IRIS Program, EPA provides the highest quality science-based human health assessments to support the...

78 FR 11808 - Approval and Promulgation of Implementation Plans; Tennessee: Approve Knox County Supplemental...

Federal Register 2010, 2011, 2012, 2013, 2014

2013-02-20

... INFORMATION: On December 18, 2012, EPA proposed to approve, through parallel processing, a draft revision to... County to account for changes in the emissions model and vehicle miles traveled projection model. EPA is... submit comments. FOR FURTHER INFORMATION CONTACT: Kelly Sheckler, Air Quality Modeling and Transportation...

Draft Genome Sequence of Corynebacterium ulcerans FRC58, Isolated from the Bronchitic Aspiration of a Patient in France

PubMed Central

Silva, Andréia do Socorro de Sousa; Baraúna, Rafael Azevedo; de Sá, Pablo Caracciolo Gomes; das Graças, Diego Assis; Carneiro, Adriana Ribeiro; Thouvenin, Maxime; Azevedo, Vasco; Badell, Edgar; Guiso, Nicole; da Silva, Artur Luiz da Costa

2014-01-01

Corynebacterium ulcerans is a bacterial species with high importance because it causes infections in animals and, rarely, in humans. Its virulence mechanisms remain unclear. The current study describes the draft genome of C. ulcerans FRC58, which was isolated from the bronchitic aspiration of a patient in France. PMID:24407640

Draft Genome Sequences of Two Protease-Producing Strains of Arsukibacterium, Isolated from Two Cold and Alkaline Environments

PubMed Central

Lylloff, Jeanette E.; Hansen, Lea B. S.; Jepsen, Morten; Hallin, Peter F.; Sørensen, Søren J.; Glaring, Mikkel A.

2015-01-01

Arsukibacterium ikkense GCM72T and a close relative, Arsukibacterium sp. MJ3, were isolated from two cold and alkaline environments as producers of extracellular proteolytic enzymes active at high pH and low temperature. This report describes the two draft genome sequences, which may serve as sources of future industrial enzymes. PMID:26044431

Draft Genome Sequences of Three Escherichia coli Strains with Different In Vivo Pathogenicities in an Avian (Ascending) Infection Model of the Oviduct.

PubMed

Olsen, Rikke Heidemann; Thøfner, Ida Cecilie Naundrup; Pors, Susanne Elisabeth; Christensen, Henrik; Bisgaard, Magne; Christensen, Jens Peter

2015-05-07

Here, we present three draft genome sequences of Escherichia coli strains that experimentally were proven to possess low (strain D2-2), intermediate (Chronic_salp), or high virulence (Cp6salp3) in an avian (ascending) infection model of the oviduct. Copyright © 2015 Olsen et al.

Computer Aided Drafting and Design, Industrial Manufacturing Technician, and Mechanical Engineering Technician and Machine Tool, Die and Moldmaking Technology. Tech Prep Competency Profile.

ERIC Educational Resources Information Center

Mid-East Ohio Tech Prep Consortium, Zanesville.

This document contains competency profiles in four areas: computer-aided drafting and design; industrial manufacturing technician; mechanical engineering technician; and machine tool, die, and moldmaking technology occupations. The profiles are intended for use in articulating tech prep programs from high school through associate degrees in Ohio.…

Draft Genome Sequence of Corynebacterium kefirresidentii SB, Isolated from Kefir.

PubMed

Blasche, Sonja; Kim, Yongkyu; Patil, Kiran R

2017-09-14

The genus Corynebacterium includes Gram-positive species with a high G+C content. We report here a novel species, Corynebacterium kefirresidentii SB, isolated from kefir grains collected in Germany. Its draft genome sequence was remarkably dissimilar (average nucleotide identity, 76.54%) to those of other Corynebacterium spp., confirming that this is a unique novel species. Copyright © 2017 Blasche et al.

Integrated Science Assessment (ISA) for Sulfur Oxides ...

EPA Pesticide Factsheets

This draft document provides EPA’s evaluation and synthesis of the most policy-relevant science related to the health effects of sulfur oxides. When final, it will provide a critical part of the scientific foundation for EPA’s decision regarding the adequacy of the current primary (health-based) National Ambient Air Quality Standard (NAAQS) for sulfur dioxide. The references considered for inclusion in or cited in the external review draft ISA are available at https://hero.epa.gov/hero/sulfur-oxides. The intent of the ISA, according to the CAA, is to “accurately reflect the latest scientific knowledge expected from the presence of [a] pollutant in ambient air” (U.S. Code, 1970a, 1970b). It includes an assessment of scientific research from atmospheric sciences, exposure sciences, dosimetry, mode of action, animal and human toxicology, and epidemiology. Key information and judgments formerly found in the Air Quality Criteria Documents (AQCDs) for sulfur oxides (SOx) are included; Annexes provide additional details supporting the ISA. Together, the ISA and Annexes serve to update and revise the last SOx ISA which was published in 2008.

Toward Global Comparability of Sexual Orientation Data in Official Statistics: A Conceptual Framework of Sexual Orientation for Health Data Collection in New Zealand's Official Statistics System

PubMed Central

Gray, Alistair; Veale, Jaimie F.; Binson, Diane; Sell, Randell L.

2013-01-01

Objective. Effectively addressing health disparities experienced by sexual minority populations requires high-quality official data on sexual orientation. We developed a conceptual framework of sexual orientation to improve the quality of sexual orientation data in New Zealand's Official Statistics System. Methods. We reviewed conceptual and methodological literature, culminating in a draft framework. To improve the framework, we held focus groups and key-informant interviews with sexual minority stakeholders and producers and consumers of official statistics. An advisory board of experts provided additional guidance. Results. The framework proposes working definitions of the sexual orientation topic and measurement concepts, describes dimensions of the measurement concepts, discusses variables framing the measurement concepts, and outlines conceptual grey areas. Conclusion. The framework proposes standard definitions and concepts for the collection of official sexual orientation data in New Zealand. It presents a model for producers of official statistics in other countries, who wish to improve the quality of health data on their citizens. PMID:23840231

Enhanced annotations and features for comparing thousands of Pseudomonas genomes in the Pseudomonas genome database.

PubMed

Winsor, Geoffrey L; Griffiths, Emma J; Lo, Raymond; Dhillon, Bhavjinder K; Shay, Julie A; Brinkman, Fiona S L

2016-01-04

The Pseudomonas Genome Database (http://www.pseudomonas.com) is well known for the application of community-based annotation approaches for producing a high-quality Pseudomonas aeruginosa PAO1 genome annotation, and facilitating whole-genome comparative analyses with other Pseudomonas strains. To aid analysis of potentially thousands of complete and draft genome assemblies, this database and analysis platform was upgraded to integrate curated genome annotations and isolate metadata with enhanced tools for larger scale comparative analysis and visualization. Manually curated gene annotations are supplemented with improved computational analyses that help identify putative drug targets and vaccine candidates or assist with evolutionary studies by identifying orthologs, pathogen-associated genes and genomic islands. The database schema has been updated to integrate isolate metadata that will facilitate more powerful analysis of genomes across datasets in the future. We continue to place an emphasis on providing high-quality updates to gene annotations through regular review of the scientific literature and using community-based approaches including a major new Pseudomonas community initiative for the assignment of high-quality gene ontology terms to genes. As we further expand from thousands of genomes, we plan to provide enhancements that will aid data visualization and analysis arising from whole-genome comparative studies including more pan-genome and population-based approaches. © The Author(s) 2015. Published by Oxford University Press on behalf of Nucleic Acids Research.

Main Ingredients for Success in L2 Academic Writing: Outlining, Drafting and Proofreading.

PubMed

Munoz-Luna, Rosa

2015-01-01

Spanish undergraduates of English Studies are required to submit their essays in academic English, a genre which most of them are not acquainted with. This paper aims to explore the extralinguistic side of second language (L2) academic writing, more specifically, the combination of metalinguistic items (e.g. transition and frame markers, among others) with students' writing strategies when composing an academic text in L2 English. The research sample conveys a group of 200 Spanish undergraduates of English Studies; they are in their fourth year, so they are expected to be proficient in English academic writing but their written production quality varies considerably. Results are analysed following a mixed methodology by which metalinguistic items are statistically measured, and then contrasted with semi-structured interview results; SPSS and NVivo provide quantitative and qualitative outcomes, respectively. The analyses reveal that undergraduate students who produce complex sentences and more coherent texts employ a wider range of writing strategies both prior and while writing, being able to (un)consciously structure and design their texts more successfully. These high-scoring students make more proficient use of complex transition markers for coherence and frame markers for textual cohesion; their commonly used (pre-)writing strategies are drafting, outlining, and proofreading.

Main Ingredients for Success in L2 Academic Writing: Outlining, Drafting and Proofreading

PubMed Central

Munoz-Luna, Rosa

2015-01-01

Spanish undergraduates of English Studies are required to submit their essays in academic English, a genre which most of them are not acquainted with. This paper aims to explore the extralinguistic side of second language (L2) academic writing, more specifically, the combination of metalinguistic items (e.g. transition and frame markers, among others) with students’ writing strategies when composing an academic text in L2 English. The research sample conveys a group of 200 Spanish undergraduates of English Studies; they are in their fourth year, so they are expected to be proficient in English academic writing but their written production quality varies considerably. Results are analysed following a mixed methodology by which metalinguistic items are statistically measured, and then contrasted with semi-structured interview results; SPSS and NVivo provide quantitative and qualitative outcomes, respectively. The analyses reveal that undergraduate students who produce complex sentences and more coherent texts employ a wider range of writing strategies both prior and while writing, being able to (un)consciously structure and design their texts more successfully. These high-scoring students make more proficient use of complex transition markers for coherence and frame markers for textual cohesion; their commonly used (pre-)writing strategies are drafting, outlining, and proofreading. PMID:26046836

Draft genome of the red harvester ant Pogonomyrmex barbatus.

PubMed

Smith, Chris R; Smith, Christopher D; Robertson, Hugh M; Helmkampf, Martin; Zimin, Aleksey; Yandell, Mark; Holt, Carson; Hu, Hao; Abouheif, Ehab; Benton, Richard; Cash, Elizabeth; Croset, Vincent; Currie, Cameron R; Elhaik, Eran; Elsik, Christine G; Favé, Marie-Julie; Fernandes, Vilaiwan; Gibson, Joshua D; Graur, Dan; Gronenberg, Wulfila; Grubbs, Kirk J; Hagen, Darren E; Viniegra, Ana Sofia Ibarraran; Johnson, Brian R; Johnson, Reed M; Khila, Abderrahman; Kim, Jay W; Mathis, Kaitlyn A; Munoz-Torres, Monica C; Murphy, Marguerite C; Mustard, Julie A; Nakamura, Rin; Niehuis, Oliver; Nigam, Surabhi; Overson, Rick P; Placek, Jennifer E; Rajakumar, Rajendhran; Reese, Justin T; Suen, Garret; Tao, Shu; Torres, Candice W; Tsutsui, Neil D; Viljakainen, Lumi; Wolschin, Florian; Gadau, Jürgen

2011-04-05

We report the draft genome sequence of the red harvester ant, Pogonomyrmex barbatus. The genome was sequenced using 454 pyrosequencing, and the current assembly and annotation were completed in less than 1 y. Analyses of conserved gene groups (more than 1,200 manually annotated genes to date) suggest a high-quality assembly and annotation comparable to recently sequenced insect genomes using Sanger sequencing. The red harvester ant is a model for studying reproductive division of labor, phenotypic plasticity, and sociogenomics. Although the genome of P. barbatus is similar to other sequenced hymenopterans (Apis mellifera and Nasonia vitripennis) in GC content and compositional organization, and possesses a complete CpG methylation toolkit, its predicted genomic CpG content differs markedly from the other hymenopterans. Gene networks involved in generating key differences between the queen and worker castes (e.g., wings and ovaries) show signatures of increased methylation and suggest that ants and bees may have independently co-opted the same gene regulatory mechanisms for reproductive division of labor. Gene family expansions (e.g., 344 functional odorant receptors) and pseudogene accumulation in chemoreception and P450 genes compared with A. mellifera and N. vitripennis are consistent with major life-history changes during the adaptive radiation of Pogonomyrmex spp., perhaps in parallel with the development of the North American deserts.

Draft Genome Sequence of Thermotoga maritima A7A Reconstructed from Metagenomic Sequencing Analysis of a Hydrocarbon Reservoir in the Bass Strait, Australia

PubMed Central

Sutcliffe, Brodie; Rosewarne, Carly P.; Greenfield, Paul; Li, Dongmei

2013-01-01

The draft genome sequence of Thermotoga maritima A7A was obtained from a metagenomic assembly obtained from a high-temperature hydrocarbon reservoir in the Gippsland Basin, Australia. The organism is predicted to be a motile anaerobe with an array of catabolic enzymes for the degradation of numerous carbohydrates. PMID:24009120

Draft Genome Sequence of Mycobacterium chimaera Type Strain Fl-0169.

PubMed

Pfaller, Stacy; Tokarev, Vasily; Kessler, Collin; McLimans, Christopher; Gomez-Alvarez, Vicente; Wright, Justin; King, Dawn; Lamendella, Regina

2017-02-23

We report here the draft genome sequence of the type strain Mycobacterium chimaera Fl-0169, a member of the Mycobacterium avium complex (MAC). M. chimaera Fl-0169 T was isolated from a patient in Italy and is highly similar to strains of M. chimaera isolated in Ireland, although Fl-0169 T possesses unique virulence genes. Copyright © 2017 Pfaller et al.

Draft Genome Sequence of Sphingobium chinhatense Strain IP26T, Isolated from a Hexachlorocyclohexane Dumpsite

PubMed Central

Niharika, Neha; Sangwan, Naseer; Ahmad, Salar; Singh, Priya; Khurana, J. P.

2013-01-01

Sphingobium chinhatense strain IP26T is a conducive hexachlorocyclohexane (HCH) degrader isolated from a heavily contaminated (450 mg HCH/g soil) HCH dumpsite. IP26T degrades α-, β-, γ-, and δ-HCH, which are highly persistent in the environment. Here we report the draft genome sequence (~5.8 Mbp) of this strain. PMID:23990581

Draft Genome Sequence of the Polyextremophilic Halorubrum sp. Strain AJ67, Isolated from Hyperarsenic Lakes in the Argentinian Puna.

PubMed

Burguener, Germán F; Maldonado, Marcos J; Revale, Santiago; Fernández Do Porto, Darío; Rascován, Nicolás; Vázquez, Martín; Farías, María Eugenia; Marti, Marcelo A; Turjanski, Adrián Gustavo

2014-02-06

Halorubrum sp. strain AJ67, an extreme halophilic UV-resistant archaeon, was isolated from Laguna Antofalla in the Argentinian Puna. The draft genome sequence suggests the presence of potent enzyme candidates that are essential for survival under multiple environmental extreme conditions, such as high UV radiation, elevated salinity, and the presence of critical arsenic concentrations.

Power break off in a bulb turbine: wall pressure sensor investigation

NASA Astrophysics Data System (ADS)

Duquesne, P.; Maciel, Y.; Aeschlimann, V.; Ciocan, G. D.; Deschênes, C.

2014-03-01

A measurement campaign using unsteady wall pressure sensors on a bulb turbine draft tube was performed over the power and efficiency break off range of a N11 curve. This study is part of the BulbT project, undertaken by the Consortium on hydraulic machines and the LAMH (Hydraulic Machine Laboratory of Laval University). The chosen operating points include the best efficiency point for a high runner blade angle and a high N11. Three other points, with the same N11, have been selected in the break off zone of the efficiency curve. Flow conditions have been set using the guide vanes while the runner blade angle remained constant. The pressure sensors were developed from small piezoresistive chips with high frequency response. The calibration gave an instrumental error lower than 0.3% of the measurement range. The unsteady wall pressure was measured simultaneously at 13 locations inside the first part of the draft tube, which is conical, and at 16 locations in the circular to rectangular transition part just downstream. It was also measured at 11 locations along a streamwise line path at the bottom left part of the draft tube, where flow separation occurs, covering the whole streamwise extent of the draft tube. For seven radial-azimuthal planes, four sensors were distributed azimuthally. As confirmed by tuft visualizations, the break off phenomenon is correlated to the presence of flow separation inside the diffuser at the wall. The break off is linked to the appearance of a large recirculation in the draft tube. The efficiency drop increases with the size of the separated region. Analysis of the draft tube pressure coefficients confirms that the break off is related to diffuser losses. The streamwise evolution of the mean pressure coefficient is analyzed for the different operating conditions. An azimuthal dissymmetry of the mean pressure produced by the separation is detected. The pressure signals have been analyzed and used to track the separation zone depending on the operating conditions. Spectral analysis of these signals reveals a low frequency unsteadiness generated by the flow separation.

Influence of upstream disturbance on the draft-tube flow of Francis turbine under part-load conditions

NASA Astrophysics Data System (ADS)

Chen, Ting; Zheng, Xianghao; Zhang, Yu-ning; Li, Shengcai

2018-02-01

Owing to the part-load operations for the enhancement of grid flexibility, the Francis turbine often suffers from severe low-frequency and large-amplitude hydraulic instability, which is mostly pertinent to the highly unsteady swirling vortex rope in the draft tube. The influence of disturbances in the upstream (e.g., large-scale vortex structures in the spiral casing) on the draft-tube vortex flow is not well understood yet. In the present paper, the influence of the upstream disturbances on the vortical flow in the draft tube is studied based on the vortex identification method and the analysis of several important parameters (e.g., the swirl number and the velocity profile). For a small guide vane opening (representing the part-load condition), the vortices triggered in the spiral casing propagate downstream and significantly affect the swirling vortex-rope precession in the draft tube, leading to the changes of the intensity and the processional frequency of the swirling vortex rope. When the guide vane opening approaches the optimum one (representing the full-load condition), the upstream disturbance becomes weaker and thus its influences on the downstream flow are very limited.

46 CFR 97.40-10 - Draft marks and draft indicating systems.

Code of Federal Regulations, 2013 CFR

2013-10-01

... 46 Shipping 4 2013-10-01 2013-10-01 false Draft marks and draft indicating systems. 97.40-10... VESSELS OPERATIONS Markings on Vessels § 97.40-10 Draft marks and draft indicating systems. (a) All vessels must have draft marks plainly and legibly visible upon the stem and upon the sternpost or...

46 CFR 196.40-10 - Draft marks and draft indicating systems.

Code of Federal Regulations, 2012 CFR

2012-10-01

... 46 Shipping 7 2012-10-01 2012-10-01 false Draft marks and draft indicating systems. 196.40-10... VESSELS OPERATIONS Markings on Vessels § 196.40-10 Draft marks and draft indicating systems. (a) All vessels must have draft marks plainly and legibly visible upon the stem and upon the sternpost or...

46 CFR 78.50-10 - Draft marks and draft indicating systems.

Code of Federal Regulations, 2013 CFR

2013-10-01

... 46 Shipping 3 2013-10-01 2013-10-01 false Draft marks and draft indicating systems. 78.50-10... OPERATIONS Markings on Vessels § 78.50-10 Draft marks and draft indicating systems. (a) All vessels must have draft marks plainly and legibly visible upon the stem and upon the sternpost or rudderpost or any place...

46 CFR 97.40-10 - Draft marks and draft indicating systems.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 46 Shipping 4 2010-10-01 2010-10-01 false Draft marks and draft indicating systems. 97.40-10... VESSELS OPERATIONS Markings on Vessels § 97.40-10 Draft marks and draft indicating systems. (a) All vessels must have draft marks plainly and legibly visible upon the stem and upon the sternpost or...

46 CFR 97.40-10 - Draft marks and draft indicating systems.

Code of Federal Regulations, 2012 CFR

2012-10-01

... 46 Shipping 4 2012-10-01 2012-10-01 false Draft marks and draft indicating systems. 97.40-10... VESSELS OPERATIONS Markings on Vessels § 97.40-10 Draft marks and draft indicating systems. (a) All vessels must have draft marks plainly and legibly visible upon the stem and upon the sternpost or...

46 CFR 196.40-10 - Draft marks and draft indicating systems.

Code of Federal Regulations, 2013 CFR

2013-10-01

... 46 Shipping 7 2013-10-01 2013-10-01 false Draft marks and draft indicating systems. 196.40-10... VESSELS OPERATIONS Markings on Vessels § 196.40-10 Draft marks and draft indicating systems. (a) All vessels must have draft marks plainly and legibly visible upon the stem and upon the sternpost or...

46 CFR 97.40-10 - Draft marks and draft indicating systems.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 46 Shipping 4 2014-10-01 2014-10-01 false Draft marks and draft indicating systems. 97.40-10... VESSELS OPERATIONS Markings on Vessels § 97.40-10 Draft marks and draft indicating systems. (a) All vessels must have draft marks plainly and legibly visible upon the stem and upon the sternpost or...

46 CFR 196.40-10 - Draft marks and draft indicating systems.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 46 Shipping 7 2011-10-01 2011-10-01 false Draft marks and draft indicating systems. 196.40-10... VESSELS OPERATIONS Markings on Vessels § 196.40-10 Draft marks and draft indicating systems. (a) All vessels must have draft marks plainly and legibly visible upon the stem and upon the sternpost or...

46 CFR 78.50-10 - Draft marks and draft indicating systems.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 46 Shipping 3 2010-10-01 2010-10-01 false Draft marks and draft indicating systems. 78.50-10... OPERATIONS Markings on Vessels § 78.50-10 Draft marks and draft indicating systems. (a) All vessels must have draft marks plainly and legibly visible upon the stem and upon the sternpost or rudderpost or any place...

46 CFR 78.50-10 - Draft marks and draft indicating systems.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 46 Shipping 3 2014-10-01 2014-10-01 false Draft marks and draft indicating systems. 78.50-10... OPERATIONS Markings on Vessels § 78.50-10 Draft marks and draft indicating systems. (a) All vessels must have draft marks plainly and legibly visible upon the stem and upon the sternpost or rudderpost or any place...

46 CFR 78.50-10 - Draft marks and draft indicating systems.

Code of Federal Regulations, 2012 CFR

2012-10-01

... 46 Shipping 3 2012-10-01 2012-10-01 false Draft marks and draft indicating systems. 78.50-10... OPERATIONS Markings on Vessels § 78.50-10 Draft marks and draft indicating systems. (a) All vessels must have draft marks plainly and legibly visible upon the stem and upon the sternpost or rudderpost or any place...

46 CFR 97.40-10 - Draft marks and draft indicating systems.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 46 Shipping 4 2011-10-01 2011-10-01 false Draft marks and draft indicating systems. 97.40-10... VESSELS OPERATIONS Markings on Vessels § 97.40-10 Draft marks and draft indicating systems. (a) All vessels must have draft marks plainly and legibly visible upon the stem and upon the sternpost or...

46 CFR 196.40-10 - Draft marks and draft indicating systems.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 46 Shipping 7 2010-10-01 2010-10-01 false Draft marks and draft indicating systems. 196.40-10... VESSELS OPERATIONS Markings on Vessels § 196.40-10 Draft marks and draft indicating systems. (a) All vessels must have draft marks plainly and legibly visible upon the stem and upon the sternpost or...

46 CFR 78.50-10 - Draft marks and draft indicating systems.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 46 Shipping 3 2011-10-01 2011-10-01 false Draft marks and draft indicating systems. 78.50-10... OPERATIONS Markings on Vessels § 78.50-10 Draft marks and draft indicating systems. (a) All vessels must have draft marks plainly and legibly visible upon the stem and upon the sternpost or rudderpost or any place...

46 CFR 196.40-10 - Draft marks and draft indicating systems.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 46 Shipping 7 2014-10-01 2014-10-01 false Draft marks and draft indicating systems. 196.40-10... VESSELS OPERATIONS Markings on Vessels § 196.40-10 Draft marks and draft indicating systems. (a) All vessels must have draft marks plainly and legibly visible upon the stem and upon the sternpost or...

Draft genome sequence of Trametes villosa (Sw.) Kreisel CCMB561, a tropical white-rot Basidiomycota from the semiarid region of Brazil.

PubMed

Ferreira, Dalila Souza Santos; Kato, Rodrigo Bentes; Miranda, Fábio Malcher; da Costa Pinheiro, Kenny; Fonseca, Paula Luize Camargos; Tomé, Luiz Marcelo Ribeiro; Vaz, Aline Bruna Martins; Badotti, Fernanda; Ramos, Rommel Thiago Jucá; Brenig, Bertram; Azevedo, Vasco Ariston de Carvalho; Benevides, Raquel Guimarães; Góes-Neto, Aristóteles

2018-06-01

Herein, we present the draft genome of Trametes villosa isolate CCMB561, a wood-decaying Basidiomycota commonly found in tropical semiarid climate. The genome assembly was 57.98 Mb in size with an L50 of 691. A total of 16,711 putative protein-encoding genes was predicted, including 590 genes coding for carbohydrate-active enzymes (CAZy), directly involved in the decomposition of lignocellulosic materials. This is the first genome of this species of high interest in bioenergy research. The draft genome of Trametes villosa isolate CCMB561 will provide an important resource for future investigations in biofuel production, bioremediation and other green technologies.

46 CFR 32.05-1 - Draft marks and draft indicating systems-TB/ALL.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 46 Shipping 1 2014-10-01 2014-10-01 false Draft marks and draft indicating systems-TB/ALL. 32.05-1..., MACHINERY, AND HULL REQUIREMENTS Markings § 32.05-1 Draft marks and draft indicating systems—TB/ALL. (a) All vessels must have draft marks plainly and legibly visible upon the stem and upon the sternpost or...

46 CFR 32.05-1 - Draft marks and draft indicating systems-TB/ALL.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 46 Shipping 1 2011-10-01 2011-10-01 false Draft marks and draft indicating systems-TB/ALL. 32.05-1..., MACHINERY, AND HULL REQUIREMENTS Markings § 32.05-1 Draft marks and draft indicating systems—TB/ALL. (a) All vessels must have draft marks plainly and legibly visible upon the stem and upon the sternpost or...

46 CFR 32.05-1 - Draft marks and draft indicating systems-TB/ALL.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 46 Shipping 1 2010-10-01 2010-10-01 false Draft marks and draft indicating systems-TB/ALL. 32.05-1..., MACHINERY, AND HULL REQUIREMENTS Markings § 32.05-1 Draft marks and draft indicating systems—TB/ALL. (a) All vessels must have draft marks plainly and legibly visible upon the stem and upon the sternpost or...

46 CFR 32.05-1 - Draft marks and draft indicating systems-TB/ALL.

Code of Federal Regulations, 2013 CFR

2013-10-01

... 46 Shipping 1 2013-10-01 2013-10-01 false Draft marks and draft indicating systems-TB/ALL. 32.05-1..., MACHINERY, AND HULL REQUIREMENTS Markings § 32.05-1 Draft marks and draft indicating systems—TB/ALL. (a) All vessels must have draft marks plainly and legibly visible upon the stem and upon the sternpost or...

46 CFR 32.05-1 - Draft marks and draft indicating systems-TB/ALL.

Code of Federal Regulations, 2012 CFR

2012-10-01

... 46 Shipping 1 2012-10-01 2012-10-01 false Draft marks and draft indicating systems-TB/ALL. 32.05-1..., MACHINERY, AND HULL REQUIREMENTS Markings § 32.05-1 Draft marks and draft indicating systems—TB/ALL. (a) All vessels must have draft marks plainly and legibly visible upon the stem and upon the sternpost or...

77 FR 51798 - First Draft Documents Related to the Review of the National Ambient Air Quality Standards for Ozone

Federal Register 2010, 2011, 2012, 2013, 2014

2012-08-27

... comment due to technical difficulties and cannot contact you for clarification, EPA may not be able to... comments, remember to: Identify the rulemaking by docket number and other identifying information (subject... 2009 and was the subject of a consultation with the Clean Air Scientific Advisory Committee (CASAC) on...

75 FR 39253 - Release of Second Draft Document Related to the Review of the National Ambient Air Quality...

Federal Register 2010, 2011, 2012, 2013, 2014

2010-07-08

... EPA cannot read your comment due to technical difficulties and cannot contact you for clarification... docket number and other identifying information (subject heading, Federal Register date and page number... October 2007 and was the subject of a consultation with the Clean Air Scientific Advisory Committee (CASAC...

76 FR 20347 - Release of Draft Integrated Review Plan for the National Ambient Air Quality Standards for Lead

Federal Register 2010, 2011, 2012, 2013, 2014

2011-04-12

... you submit. If EPA cannot read your comment due to technical difficulties and cannot contact you for... rulemaking by docket number and other identifying information (subject heading, Federal Register date and... phase for the review. This phase began with a science policy workshop to identify issues and questions...

Assessing the Relationship between Different Types of Student Feedback and the Quality of Revised Writing

ERIC Educational Resources Information Center

Diab, Nuwar Mawlawi

2011-01-01

This paper reports on a quasi-experimental study comparing the effects of peer-editing to self-editing on improving students' revised drafts. The study involved two intact classes (experimental and control groups) of an English course. The experimental group practiced peer-editing while the control group engaged in self-editing. After receiving…

76 FR 549 - Clean Water Act Section 303(d): Notice for the Establishment of the Total Maximum Daily Load...

Federal Register 2010, 2011, 2012, 2013, 2014

2011-01-05

... EPA's establishment of the Chesapeake Bay (Bay) TMDL on December 29, 2010 for nitrogen, phosphorus and... revised the draft TMDL as appropriate and established the Bay TMDL for nitrogen, phosphorus and sediment..., phosphorus and sediment which can enter a waterbody without causing a violation in the water quality...

75 FR 57056 - Notice of Availability of the Draft Buffalo Resource Management Plan Amendment for the...

Federal Register 2010, 2011, 2012, 2013, 2014

2010-09-17

... further information contact Thomas Bills, Buffalo RMPA Team Leader, telephone at 307-684-1133; mailing..., threatened and endangered species, visual resources, air quality, wilderness characteristics, and other.... Donald A. Simpson, State Director. [FR Doc. 2010-23330 Filed 9-16-10; 8:45 am] BILLING CODE 4310-22-P ...

78 FR 39010 - Notice of Availability of the Draft Resource Management Plan and Associated Environmental Impact...

Federal Register 2010, 2011, 2012, 2013, 2014

2013-06-28

...: Thomas Bills, Buffalo RMP Team Leader, BLM Buffalo Field Office, 1425 Fort Street, Buffalo, WY 82834... sage-grouse, livestock grazing, air quality, lands with wilderness characteristics, suitability for... CFR 1506.10, 43 CFR 1610.2. Donald A. Simpson, State Director, Wyoming. [FR Doc. 2013-15381 Filed 6-27...

76 FR 28505 - Okanogan Public Utility District No. 1 of Okanogan County, WA; Notice of Availability of Draft...

Federal Register 2010, 2011, 2012, 2013, 2014

2011-05-17

....5 miles of new and upgraded access roads. The Enloe Project would operate automatically in a run-of... run-of-river and implementing agency-recommended ramping rates downstream of the project during... effects on geology and soils and water quality. Run-of-river operation would minimize effects on aquatic...

78 FR 42928 - Draft Environmental Assessment for the Cotton Quality Research Station Land Transfer

Federal Register 2010, 2011, 2012, 2013, 2014

2013-07-18

... Research Station (CQRS) from the USDA Agricultural Research Service (ARS) in Clemson, South Carolina, to... comments related to the proposed CQRS Land Transfer by any of the following methods: Email: [email protected]ars.usda.gov , Fax: 309-681-6683. Mail: USDA-ARS-SHEMB, NCAUR, 1815 North University Avenue, Room 2016...

77 FR 72820 - Hot-Rolled Flat-Rolled Carbon-Quality Steel Products From the Russian Federation; 2010-2011...

Federal Register 2010, 2011, 2012, 2013, 2014

2012-12-06

... Ministry of Russia'') initialed a draft revision to the Agreement which realigns the reference prices... 30, 2012, the Department and The Economy Ministry of Russia signed the final revision to the... date, the Department revoked Russia's status as a non-market-economy country under section 771(18)(B...

77 FR 38882 - Notice of Availability of a Draft Environmental Impact Statement, for the Chicago, IL to St...

Federal Register 2010, 2011, 2012, 2013, 2014

2012-06-29

... INFORMATION CONTACT: Andrea E. Martin, Environmental Protection Specialist, Office of Railroad Policy and... Environmental Policy Act of 1969 (NEPA) (42 U.S.C. 4321 et seq.), the Council on Environmental Quality (CEQ... viewing at the following locations along the Corridor: Hayner Library, 326 Belle Street, Alton, IL 62002...

Text Revision in Deaf and Hearing Bilingual Students

ERIC Educational Resources Information Center

Teruggi, Lilia A.; Gutiérrez-Cáceres, Rafaela

2016-01-01

In this study we explored the revision process and strategies implemented by deaf and hearing students who attend the same bilingual school context (LIS and Italian). For that we analysed and compared the types and quality of revisions made by deaf and hearing participants to their first draft of a narrative text ("Frog, Where Are You?")…

77 FR 48131 - Draft Finding of No Significant Impact and Programmatic Environmental Assessment for the...

Federal Register 2010, 2011, 2012, 2013, 2014

2012-08-13

... consumption of freshwater resources and returning water back to the same watershed so as not to deplete the groundwater and surface water resources of that region in quantity or quality; and (3) Reducing, reusing, and... and behavior as well as multiple possible projects and technologies to enhance resource efficiency...

75 FR 1802 - Termination of the Environmental Impact Statement for the General Management Plan, Gila Cliff...

Federal Register 2010, 2011, 2012, 2013, 2014

2010-01-13

... Management Plan because it has determined that an Environmental Assessment (EA) is the more appropriate... Assessment was approved by the Chief of the NPS Environmental Quality Division on November 4, 2009. The draft general management plan and Environmental Assessment is expected to be distributed for a 30 day public...

Multiple Alternatives Modeling in Determining Fiscal Roll-Backs during Educational Funding Crises. Interim Draft. Paper and Report Series No. 70.

ERIC Educational Resources Information Center

Wholeben, Brent E.; Sullivan, John M.

This report provides an extensive discussion of the use of criterion referenced, mathematical modeling procedures to determine which budget reductions minimize reduction in the quality of educational programs. Part I, "Evaluation of Potential Budgeting Roll-backs under Educational Fiscal Crisis," explains the basic design of multiple…

Environmental Quality Technology Demonstration, Evaluation and Transfer Activities

DTIC Science & Technology

1996-12-02

and Appropriate Requirements) and ARAR support documentation for interim and final Installation Restoration Program (IRP) remedial actions . During the...Relevant and Appropriate Requirements (ARARs) for Fort Devens , Massachusetts. Delivered Federal Environmental Legislative Power Curve in hard copy and... Remedial Actions . Delivered revised tables for Desk Guide on Interpretation of Applicable or Relevant and Appropriate Requirements — Interim Draft

The Oral Language Process in Writing: A Real-Life Writing Session.

ERIC Educational Resources Information Center

Shuy, Roger W.; Robinson, David G.

1990-01-01

Analyzes a real-life writing session involving a male executive in the construction business, his female secretary, and a male representing himself as a state official, working collaboratively to write a letter to a state official urging action on a long overdue claim. Discusses the quality of the drafts and the participants' roles. (KEH)

NIH state-of-the-science conference statement: Enhancing use and quality of colorectal cancer screening.

PubMed

Steinwachs, Donald; Allen, Jennifer Dacey; Barlow, William Eric; Duncan, R Paul; Egede, Leonard E; Friedman, Lawrence S; Keating, Nancy L; Kim, Paula; Lave, Judith R; LaVeist, Thomas A; Ness, Roberta B; Optican, Robert J; Virnig, Beth A

2010-02-04

To provide health care providers, patients, and the general public with a responsible assessment of currently available data on enhancing use and quality of colorectal cancer screening. A non-DHHS, nonadvocate 13-member panel representing the fields of cancer surveillance, health services research, community-based research, informed decision-making, access to care, health care policy, health communication, health economics, health disparities, epidemiology, statistics, thoracic radiology, internal medicine, gastroenterology, public health, end-of-life care, and a public representative. In addition, 20 experts from pertinent fields presented data to the panel and conference audience. Presentations by experts and a systematic review of the literature prepared by the RTI International-University of North Carolina Evidence-based Practice Center, through the Agency for Healthcare Research and Quality. Scientific evidence was given precedence over anecdotal experience. The panel drafted its statement based on scientific evidence presented in open forum and on published scientific literature. The draft statement was presented on the final day of the conference and circulated to the audience for comment. The panel released a revised statement later that day at http://consensus.nih.gov. This statement is an independent report of the panel and is not a policy statement of the NIH or the Federal Government. The panel found that despite substantial progress toward higher colorectal cancer screening rates nationally, screening rates fall short of desirable levels. Targeted initiatives to improve screening rates and reduce disparities in underscreened communities and population subgroups could further reduce colorectal cancer morbidity and mortality. This could be achieved by utilizing the full range of screening options and evidence-based interventions for increasing screening rates. With additional investments in quality monitoring, Americans could be assured that all screening achieves high rates of cancer prevention and early detection. To close the gap in screening, this report identifies the following priority areas for implementation and research to enhance the use and quality of colorectal cancer screening: • Eliminate financial barriers to colorectal cancer screening and appropriate follow up. • Widely implement interventions that have proven effective at increasing colorectal cancer screening, including patient reminder systems and one-on-one interactions with providers, educators, or navigators. • Conduct research to assess the effectiveness of tailoring programs to match the characteristics and preferences of target population groups to increase colorectal cancer screening. • Implement systems to ensure appropriate follow-up of positive colorectal cancer screening results. • Develop systems to assure high quality of colorectal cancer screening programs. • Conduct studies to determine the comparative effectiveness of the various colorectal cancer screening methods in usual practice settings.

Managing uncertainty in metabolic network structure and improving predictions using EnsembleFBA

PubMed Central

2017-01-01

Genome-scale metabolic network reconstructions (GENREs) are repositories of knowledge about the metabolic processes that occur in an organism. GENREs have been used to discover and interpret metabolic functions, and to engineer novel network structures. A major barrier preventing more widespread use of GENREs, particularly to study non-model organisms, is the extensive time required to produce a high-quality GENRE. Many automated approaches have been developed which reduce this time requirement, but automatically-reconstructed draft GENREs still require curation before useful predictions can be made. We present a novel approach to the analysis of GENREs which improves the predictive capabilities of draft GENREs by representing many alternative network structures, all equally consistent with available data, and generating predictions from this ensemble. This ensemble approach is compatible with many reconstruction methods. We refer to this new approach as Ensemble Flux Balance Analysis (EnsembleFBA). We validate EnsembleFBA by predicting growth and gene essentiality in the model organism Pseudomonas aeruginosa UCBPP-PA14. We demonstrate how EnsembleFBA can be included in a systems biology workflow by predicting essential genes in six Streptococcus species and mapping the essential genes to small molecule ligands from DrugBank. We found that some metabolic subsystems contributed disproportionately to the set of predicted essential reactions in a way that was unique to each Streptococcus species, leading to species-specific outcomes from small molecule interactions. Through our analyses of P. aeruginosa and six Streptococci, we show that ensembles increase the quality of predictions without drastically increasing reconstruction time, thus making GENRE approaches more practical for applications which require predictions for many non-model organisms. All of our functions and accompanying example code are available in an open online repository. PMID:28263984

Managing uncertainty in metabolic network structure and improving predictions using EnsembleFBA.

PubMed

Biggs, Matthew B; Papin, Jason A

2017-03-01

Genome-scale metabolic network reconstructions (GENREs) are repositories of knowledge about the metabolic processes that occur in an organism. GENREs have been used to discover and interpret metabolic functions, and to engineer novel network structures. A major barrier preventing more widespread use of GENREs, particularly to study non-model organisms, is the extensive time required to produce a high-quality GENRE. Many automated approaches have been developed which reduce this time requirement, but automatically-reconstructed draft GENREs still require curation before useful predictions can be made. We present a novel approach to the analysis of GENREs which improves the predictive capabilities of draft GENREs by representing many alternative network structures, all equally consistent with available data, and generating predictions from this ensemble. This ensemble approach is compatible with many reconstruction methods. We refer to this new approach as Ensemble Flux Balance Analysis (EnsembleFBA). We validate EnsembleFBA by predicting growth and gene essentiality in the model organism Pseudomonas aeruginosa UCBPP-PA14. We demonstrate how EnsembleFBA can be included in a systems biology workflow by predicting essential genes in six Streptococcus species and mapping the essential genes to small molecule ligands from DrugBank. We found that some metabolic subsystems contributed disproportionately to the set of predicted essential reactions in a way that was unique to each Streptococcus species, leading to species-specific outcomes from small molecule interactions. Through our analyses of P. aeruginosa and six Streptococci, we show that ensembles increase the quality of predictions without drastically increasing reconstruction time, thus making GENRE approaches more practical for applications which require predictions for many non-model organisms. All of our functions and accompanying example code are available in an open online repository.

Drafting Fundamentals. Drafting Module 1. Instructor's Guide.

ERIC Educational Resources Information Center

Missouri Univ., Columbia. Instructional Materials Lab.

This Missouri Vocational Instruction Management System instructor's drafting guide has been keyed to the drafting competency profile developed by state industry and education professionals. The guide contains a cross-reference table of instructional materials. Ten units cover drafting fundamentals: (1) introduction to drafting; (2) general safety;…

Draft Genome Sequence of Thermoanaerobacter sp. Strain A7A, Reconstructed from a Metagenome Obtained from a High-Temperature Hydrocarbon Reservoir in the Bass Strait, Australia

PubMed Central

Li, Dongmei; Greenfield, Paul; Rosewarne, Carly P.

2013-01-01

The draft genome sequence of Thermoanaerobacter sp. strain A7A was reconstructed from a metagenome of a microbial consortium obtained from the Tuna oil field in the Gippsland Basin, Australia. The organism is a strict anaerobe that is predicted to ferment a range of simple sugars and undertake sulfur reduction. PMID:24029756

Draft Genome Sequence of the Polyextremophilic Exiguobacterium sp. Strain S17, Isolated from Hyperarsenic Lakes in the Argentinian Puna.

PubMed

Ordoñez, Omar F; Lanzarotti, Esteban; Kurth, Daniel; Gorriti, Marta F; Revale, Santiago; Cortez, Néstor; Vazquez, Martin P; Farías, María E; Turjanski, Adrian G

2013-07-25

Exiguobacterium sp. strain S17 is a moderately halotolerant, arsenic-resistant bacterium that was isolated from Laguna Socompa stromatolites in the Argentinian Puna. The draft genome sequence suggests potent enzyme candidates that are essential for survival under multiple environmental extreme conditions, such as high levels of UV radiation, elevated salinity, and the presence of critical arsenic concentrations.

Draft Genome Sequence of the Polyextremophilic Halorubrum sp. Strain AJ67, Isolated from Hyperarsenic Lakes in the Argentinian Puna

PubMed Central

Burguener, Germán F.; Maldonado, Marcos J.; Revale, Santiago; Fernández Do Porto, Darío; Rascován, Nicolás; Vázquez, Martín; Farías, María Eugenia; Marti, Marcelo A.

2014-01-01

Halorubrum sp. strain AJ67, an extreme halophilic UV-resistant archaeon, was isolated from Laguna Antofalla in the Argentinian Puna. The draft genome sequence suggests the presence of potent enzyme candidates that are essential for survival under multiple environmental extreme conditions, such as high UV radiation, elevated salinity, and the presence of critical arsenic concentrations. PMID:24503991

United Kingdom unveils ambitious air pollution plan

NASA Astrophysics Data System (ADS)

Warren, Matthew

2018-06-01

The U.K. government's new strategy to combat air pollution has drawn praise for its ambitious goals—and reservations about whether they will be achieved. Environmental scientists have applauded the aims of the Clean Air Strategy, which include substantially reducing the number of people breathing air containing high levels of fine particulates and curbing ammonia emissions from agriculture. But for now, the document, published as a draft for public consultation on 22 May, remains light on the specific policies that will help the country attain these goals. And although the government has said that with the new strategy it will go further than the European Union in tackling air pollution post-Brexit, scientists say major improvements to air quality will require a coordinated effort across Europe.

Evaluation of microfluidic channels with optical coherence tomography

NASA Astrophysics Data System (ADS)

Czajkowski, J.; Prykäri, T.; Alarousu, E.; Lauri, J.; Myllylä, R.

2010-11-01

Application of time domain, ultra high resolution optical coherence tomography (UHR-OCT) in evaluation of microfluidic channels is demonstrated. Presented study was done using experimental UHR-OCT device based on a Kerr-lens mode locked Ti:sapphire femtosecond laser, a photonic crystal fibre and modified, free-space Michelson interferometer. To show potential of the technique, microfluidic chip fabricated by VTT Center for Printed Intelligence (Oulu, Finland) was measured. Ability for full volumetric reconstruction in non-contact manner enabled complete characterization of closed entity of a microfluidic channel without contamination and harm for the sample. Measurement, occurring problems, and methods of postprocessing for raw data are described. Results present completely resolved physical structure of the channel, its spatial dimensions, draft angles and evaluation of lamination quality.

An empirical definition of clinical supervision.

PubMed

Milne, Derek

2007-11-01

The growing recognition of clinical supervision as the basis for high-quality mental health services is apparent in policy, research and clinical practice, but an empirical definition is required to progress research and practice. A logical analysis was used to draft a working definition, and then a systematic review of 24 empirical studies of clinical supervision produced a best evidence synthesis, which was used to test and improve this definition. The logical analysis indicated that the most popular definition (Bernard & Goodyear, 1992) failed all four necessary tests of a good definition: precision, specification, operationalization and corroboration. The systematic review synthesis was then used to test the working definition, which passed these tests (with two amendments). These two complementary review approaches created a firmer basis for advancing research and practice.

Functional Drafting, Drafting--Intermediate: 9255.02.

ERIC Educational Resources Information Center

Dade County Public Schools, Miami, FL.

The course consists of six instructional blocks totaling 135 clock hours: (1) functional drafting, (2) threads and fasteners, (3) pictorial drawings, (4) introduction to electronic drafting, (5) introduction to piping drafting, and (6) Quinmester posttest. Mastery of skills indicated in Drafting-Basic--9255.01 is a prerequisite. In the functional…

76 FR 41808 - Draft Environmental Impact Statement and Draft Habitat Conservation Plan for Oncor Electric...

Federal Register 2010, 2011, 2012, 2013, 2014

2011-07-15

...] Draft Environmental Impact Statement and Draft Habitat Conservation Plan for Oncor Electric Delivery..., announce the availability of the draft environmental impact statement and the draft Oncor Electric Delivery... Oncor Electric Delivery Company, LLC (Applicant; Oncor), and (2) That the Applicant has developed a...