Auditory Temporal Resolution in Individuals with Diabetes Mellitus Type 2.

PubMed

Mishra, Rajkishor; Sanju, Himanshu Kumar; Kumar, Prawin

2016-10-01

Introduction  "Diabetes mellitus is a group of metabolic disorders characterized by elevated blood sugar and abnormalities in insulin secretion and action" (American Diabetes Association). Previous literature has reported connection between diabetes mellitus and hearing impairment. There is a dearth of literature on auditory temporal resolution ability in individuals with diabetes mellitus type 2. Objective  The main objective of the present study was to assess auditory temporal resolution ability through GDT (Gap Detection Threshold) in individuals with diabetes mellitus type 2 with high frequency hearing loss. Methods  Fifteen subjects with diabetes mellitus type 2 with high frequency hearing loss in the age range of 30 to 40 years participated in the study as the experimental group. Fifteen age-matched non-diabetic individuals with normal hearing served as the control group. We administered the Gap Detection Threshold (GDT) test to all participants to assess their temporal resolution ability. Result  We used the independent t -test to compare between groups. Results showed that the diabetic group (experimental) performed significantly poorer compared with the non-diabetic group (control). Conclusion  It is possible to conclude that widening of auditory filters and changes in the central auditory nervous system contributed to poorer performance for temporal resolution task (Gap Detection Threshold) in individuals with diabetes mellitus type 2. Findings of the present study revealed the deteriorating effect of diabetes mellitus type 2 at the central auditory processing level.

AIRS Subpixel Cloud Characterization Using MODIS Cloud Products.

NASA Astrophysics Data System (ADS)

Li, Jun; Menzel, W. Paul; Sun, Fengying; Schmit, Timothy J.; Gurka, James

2004-08-01

The Moderate Resolution Imaging Spectroradiometer (MODIS) and the Atmospheric Infrared Sounder (AIRS) measurements from the Earth Observing System's (EOS's) Aqua satellite enable improved global monitoring of the distribution of clouds. MODIS is able to provide, at high spatial resolution (1 5 km), a cloud mask, surface and cloud types, cloud phase, cloud-top pressure (CTP), effective cloud amount (ECA), cloud particle size (CPS), and cloud optical thickness (COT). AIRS is able to provide CTP, ECA, CPS, and COT at coarser spatial resolution (13.5 km at nadir) but with much better accuracy using its high-spectral-resolution measurements. The combined MODIS AIRS system offers the opportunity for improved cloud products over those possible from either system alone. The key steps for synergistic use of imager and sounder radiance measurements are 1) collocation in space and time and 2) imager cloud amount, type, and phase determination within the sounder pixel. The MODIS and AIRS measurements from the EOS Aqua satellite provide the opportunity to study the synergistic use of advanced imager and sounder measurements. As the first step, the MODIS classification procedure is applied to identify various surface and cloud types within an AIRS footprint. Cloud-layer information (lower, midlevel, or high clouds) and phase information (water, ice, or mixed-phase clouds) within the AIRS footprint are sorted and characterized using MODIS 1-km-spatial-resolution data. The combined MODIS and AIRS data for various scenes are analyzed to study the utility of the synergistic use of high-spatial-resolution imager products and high-spectral-resolution sounder radiance measurements. There is relevance to the optimal use of data from the Advanced Baseline Imager (ABI) and Hyperspectral Environmental Suite (HES) systems, which are to fly on the Geostationary Operational Environmental Satellite (GOES)-R.

High-resolution, cryogenic, side-entry type specimen stage

DOEpatents

King, Wayne E.; Merkle, Karl L.

1979-01-01

A high-resolution, cryogenic side-entry type specimen stage includes a copper block within which a specimen can be positioned in the electron beam of an electron microscope, one end of the copper block constituting a specimen heat exchanger, means for directing a flow of helium at cryogenic temperature into the heat exchanger, and electrical leads running from the specimen to the exterior of the microscope for four point D.C. electrical resistivity measurements.

Immunohistochemical myofiber typing and high-resolution myofibrillar lesion detection in LR white embedded muscle

NASA Technical Reports Server (NTRS)

Thompson, J. L.; Vijayan, K.; Riley, D. A.

2000-01-01

We have developed a method of fixing, embedding, sectioning, and staining that allows high-resolution detection of myofibrillar structure and myosin immunocytochemical muscle fiber typing in serial semithin sections of LR White plastic embedded muscle at the light microscopic level. Traditional approaches, such as cryostat sections, permit fiber typing, but small myofibrillar lesions (1-3 sarcomeres) are difficult to detect because of section thickness. Semithin sections of hydrophobic resins do not stain well either histochemically or immunocytochemically. Electron microscopy can resolve lesions and discriminate fiber types based on morphology, but the sampling area is small. Our goal was to develop a rapid method for defining both fiber type and high-resolution primary myofibrillar lesion damage. Mild fixation (1-4% paraformaldehyde, 0. 05-0.1% glutaraldehyde) and embedment in a hydrophilic resin (LR White) were used. Myofibrillar structure was extremely well preserved at the light microscopic (LM) level, and lesions could be readily resolved in Toluidine blue stained 500-nm sections. Fiber type was defined by LM immunomyosin staining of serial plastic semithin sections, which demonstrated reciprocal staining patterns for "fast (Sigma M4276) and "total" (skeletal muscle) myosins (Sigma M7523). Copyright 2000 Wiley-Liss, Inc.

Classification of human coronary atherosclerotic plaques using ex vivo high-resolution multicontrast-weighted MRI compared with histopathology.

PubMed

Li, Tao; Li, Xin; Zhao, Xihai; Zhou, Weihua; Cai, Zulong; Yang, Li; Guo, Aitao; Zhao, Shaohong

2012-05-01

The objective of our study was to evaluate the feasibility of ex vivo high-resolution multicontrast-weighted MRI to accurately classify human coronary atherosclerotic plaques according to the American Heart Association classification. Thirteen human cadaver heart specimens were imaged using high-resolution multicontrast-weighted MR technique (T1-weighted, proton density-weighted, and T2-weighted). All MR images were matched with histopathologic sections according to the landmark of the bifurcation of the left main coronary artery. The sensitivity and specificity of MRI for the classification of plaques were determined, and Cohen's kappa analysis was applied to evaluate the agreement between MRI and histopathology in the classification of atherosclerotic plaques. One hundred eleven MR cross-sectional images obtained perpendicular to the long axis of the proximal left anterior descending artery were successfully matched with the histopathologic sections. For the classification of plaques, the sensitivity and specificity of MRI were as follows: type I-II (near normal), 60% and 100%; type III (focal lipid pool), 80% and 100%; type IV-V (lipid, necrosis, fibrosis), 96.2% and 88.2%; type VI (hemorrhage), 100% and 99.0%; type VII (calcification), 93% and 100%; and type VIII (fibrosis without lipid core), 100% and 99.1%, respectively. Isointensity, which indicates lipid composition on histopathology, was detected on MRI in 48.8% of calcified plaques. Agreement between MRI and histopathology for plaque classification was 0.86 (p < 0.001). Ex vivo high-resolution multicontrast-weighted MRI can accurately classify advanced atherosclerotic plaques in human coronary arteries.

Nuclear and chloroplast DNA differentiation in Andean potatoes.

PubMed

Sukhotu, Thitaporn; Kamijima, Osamu; Hosaka, Kazuyoshi

2004-02-01

Over 3500 accessions of Andean landraces have been known in potato, classified into 7 cultivated species ranging from 2x to 5x (Hawkes 1990). Chloroplast DNA (ctDNA), distinguished into T, W, C, S, and A types, showed extensive overlaps in their frequencies among cultivated species and between cultivated and putative ancestral wild species. In this study, 76 accessions of cultivated and 19 accessions of wild species were evaluated for ctDNA types and examined by ctDNA high-resolution markers (ctDNA microsatellites and H3 marker) and nuclear DNA restriction fragment length polymorphisms (RFLPs). ctDNA high-resolution markers identified 25 different ctDNA haplotypes. The S- and A-type ctDNAs were discriminated as unique haplotypes from 12 haplotypes having C-type ctDNA and T-type ctDNA from 10 haplotypes having W-type ctDNA. Differences among ctDNA types were strongly correlated with those of ctDNA high-resolution markers (r = 0.822). Differentiation between W-type ctDNA and C-, S-, and A-type ctDNAs was supported by nDNA RFLPs in most species except for those of recent or immediate hybrid origin. However, differentiation among C-, S-, and A-type ctDNAs was not clearly supported by nDNA RFLPs, suggesting that frequent genetic exchange occurred among them and (or) they shared the same gene pool owing to common ancestry.

Enhancing Hi-C data resolution with deep convolutional neural network HiCPlus.

PubMed

Zhang, Yan; An, Lin; Xu, Jie; Zhang, Bo; Zheng, W Jim; Hu, Ming; Tang, Jijun; Yue, Feng

2018-02-21

Although Hi-C technology is one of the most popular tools for studying 3D genome organization, due to sequencing cost, the resolution of most Hi-C datasets are coarse and cannot be used to link distal regulatory elements to their target genes. Here we develop HiCPlus, a computational approach based on deep convolutional neural network, to infer high-resolution Hi-C interaction matrices from low-resolution Hi-C data. We demonstrate that HiCPlus can impute interaction matrices highly similar to the original ones, while only using 1/16 of the original sequencing reads. We show that the models learned from one cell type can be applied to make predictions in other cell or tissue types. Our work not only provides a computational framework to enhance Hi-C data resolution but also reveals features underlying the formation of 3D chromatin interactions.

Spectroscopic Characterisation of CARMENES Target Candidates from FEROS, CAFE and HRS High-Resolution Spectra

NASA Astrophysics Data System (ADS)

Passegger, Vera Maria; Reiners, Ansgar; Jeffers, Sandra V.; Wende, Sebastian; Schöfer, Patrick; Amado, Pedro J.; Caballero, Jose A.; Montes, David; Mundt, Reinhard; Ribas, Ignasi; Quirrenbach, Andreas

2016-07-01

CARMENES (Calar Alto high-Resolution search for M dwarfs with Exoearths with Near-infrared and optical Échelle Spectrographs) started a new planet survey on M-dwarfs in January this year. The new high-resolution spectrographs are operating in the visible and near-infrared at Calar Alto Observatory. They will perform high-accuracy radial-velocity measurements (goal 1 m s-1) of about 300 M-dwarfs with the aim to detect low-mass planets within habitable zones. We characterised the candidate sample for CARMENES and provide fundamental parameters for these stars in order to constrain planetary properties and understand star-planet systems. Using state-of-the-art model atmospheres (PHOENIX-ACES) and χ2-minimization with a downhill-simplex method we determine effective temperature, surface gravity and metallicity [Fe/H] for high-resolution spectra of around 480 stars of spectral types M0.0-6.5V taken with FEROS, CAFE and HRS. We find good agreement between the models and our observed high-resolution spectra. We show the performance of the algorithm, as well as results, parameter and spectral type distributions for the CARMENES candidate sample, which is used to define the CARMENES target sample. We also present first preliminary results obtained from CARMENES spectra.

Room temperature X- and gamma-ray detectors using thallium bromide crystals

NASA Astrophysics Data System (ADS)

Hitomi, K.; Muroi, O.; Shoji, T.; Suehiro, T.; Hiratate, Y.

1999-10-01

Thallium bromide (TlBr) is a compound semiconductor with wide band gap (2.68eV) and high X- and γ-ray stopping power. The TlBr crystals were grown by the horizontal travelling molten zone (TMZ) method using purified material. Two types of room temperature X- and γ-ray detectors were fabricated from the TlBr crystals: TlBr detectors with high detection efficiency for positron annihilation γ-ray (511keV) detection and TlBr detectors with high-energy resolution for low-energy X-ray detection. The detector of the former type demonstrated energy resolution of 56keV FWHM (11%) for 511keV γ-rays. Energy resolution of 1.81keV FWHM for 5.9keV was obtained from the detector of the latter type. In order to analyze noise characteristics of the detector-preamplifier assembly, the equivalent noise charge (ENC) was measured as a function of the amplifier shaping time for the high-resolution detector. This analysis shows that parallel white noise and /1/f noise were dominant noise sources in the detector system. Current-voltage characteristics of the TlBr detector with a small Peltier cooler were also measured. Significant reduction of the detector leakage current was observed for the cooled detectors.

Prescription of land-surface boundary conditions in GISS GCM 2: A simple method based on high-resolution vegetation data bases

NASA Technical Reports Server (NTRS)

Matthews, E.

1984-01-01

A simple method was developed for improved prescription of seasonal surface characteristics and parameterization of land-surface processes in climate models. This method, developed for the Goddard Institute for Space Studies General Circulation Model II (GISS GCM II), maintains the spatial variability of fine-resolution land-cover data while restricting to 8 the number of vegetation types handled in the model. This was achieved by: redefining the large number of vegetation classes in the 1 deg x 1 deg resolution Matthews (1983) vegetation data base as percentages of 8 simple types; deriving roughness length, field capacity, masking depth and seasonal, spectral reflectivity for the 8 types; and aggregating these surface features from the 1 deg x 1 deg resolution to coarser model resolutions, e.g., 8 deg latitude x 10 deg longitude or 4 deg latitude x 5 deg longitude.

Development of a sub-cm high resolution ion Doppler tomography diagnostics for fine structure measurement of guide field reconnection in TS-U

NASA Astrophysics Data System (ADS)

Tanabe, Hiroshi; Koike, Hideya; Hatano, Hironori; Hayashi, Takumi; Cao, Qinghong; Himeno, Shunichi; Kaneda, Taishi; Akimitsu, Moe; Sawada, Asuka; Ono, Yasushi

2017-10-01

A new type of high-throughput/high-resolution 96CH ion Doppler tomography diagnostics has been developed using ``multi-slit'' spectroscopy technique for detailed investigation of fine structure formation during high guide field magnetic reconnection. In the last three years, high field merging experiment in MAST pioneered new frontiers of reconnection heating: formation of highly peaked structure around X-point in high guide field condition (Bt > 0.3 T), outflow dissipation under the influence of better plasma confinement to form high temperature ring structure which aligns with closed flux surface of toroidal plasma, and interaction between ion and electron temperature profile during transport/confinement phase to form triple peak structure (τeiE 4 ms). To investigate more detailed mechanism with in-situ magnetic measurement, the university of Tokyo starts the upgrade of plasma parameters and spatial resolution of optical diagnostics as in MAST. Now, a new type of high-throughput/high-resolution 96CH ion Doppler tomography diagnostics system construction has been completed and it successfully resolved fine structure of ion heating downstream, aligned with closed flux surface formed by reconnected field. This work was supported by JSPS KAKENHI Grant Numbers 15H05750, 15K14279 and 17H04863.

Development of a high spectral resolution surface albedo product for the ARM Southern Great Plains central facility

NASA Astrophysics Data System (ADS)

McFarlane, S. A.; Gaustad, K. L.; Mlawer, E. J.; Long, C. N.; Delamere, J.

2011-09-01

We present a method for identifying dominant surface type and estimating high spectral resolution surface albedo at the Atmospheric Radiation Measurement (ARM) facility at the Southern Great Plains (SGP) site in Oklahoma for use in radiative transfer calculations. Given a set of 6-channel narrowband visible and near-infrared irradiance measurements from upward and downward looking multi-filter radiometers (MFRs), four different surface types (snow-covered, green vegetation, partial vegetation, non-vegetated) can be identified. A normalized difference vegetation index (NDVI) is used to distinguish between vegetated and non-vegetated surfaces, and a scaled NDVI index is used to estimate the percentage of green vegetation in partially vegetated surfaces. Based on libraries of spectral albedo measurements, a piecewise continuous function is developed to estimate the high spectral resolution surface albedo for each surface type given the MFR albedo values as input. For partially vegetated surfaces, the albedo is estimated as a linear combination of the green vegetation and non-vegetated surface albedo values. The estimated albedo values are evaluated through comparison to high spectral resolution albedo measurements taken during several Intensive Observational Periods (IOPs) and through comparison of the integrated spectral albedo values to observed broadband albedo measurements. The estimated spectral albedo values agree well with observations for the visible wavelengths constrained by the MFR measurements, but have larger biases and variability at longer wavelengths. Additional MFR channels at 1100 nm and/or 1600 nm would help constrain the high resolution spectral albedo in the near infrared region.

Development of a high spectral resolution surface albedo product for the ARM Southern Great Plains central facility

NASA Astrophysics Data System (ADS)

McFarlane, S. A.; Gaustad, K. L.; Mlawer, E. J.; Long, C. N.; Delamere, J.

2011-05-01

We present a method for identifying dominant surface type and estimating high spectral resolution surface albedo at the Atmospheric Radiation Measurement (ARM) facility at the Southern Great Plains (SGP) site in Oklahoma for use in radiative transfer calculations. Given a set of 6-channel narrowband visible and near-infrared irradiance measurements from upward and downward looking multi-filter radiometers (MFRs), four different surface types (snow-covered, green vegetation, partial vegetation, non-vegetated) can be identified. A normalized difference vegetation index (NDVI) is used to distinguish between vegetated and non-vegetated surfaces, and a scaled NDVI index is used to estimate the percentage of green vegetation in partially vegetated surfaces. Based on libraries of spectral albedo measurements, a piecewise continuous function is developed to estimate the high spectral resolution surface albedo for each surface type given the MFR albedo values as input. For partially vegetated surfaces, the albedo is estimated as a linear combination of the green vegetation and non-vegetated surface albedo values. The estimated albedo values are evaluated through comparison to high spectral resolution albedo measurements taken during several Intensive Observational Periods (IOPs) and through comparison of the integrated spectral albedo values to observed broadband albedo measurements. The estimated spectral albedo values agree well with observations for the visible wavelengths constrained by the MFR measurements, but have larger biases and variability at longer wavelengths. Additional MFR channels at 1100 nm and/or 1600 nm would help constrain the high resolution spectral albedo in the near infrared region.

High-resolution separation of neodymium and dysprosium ions utilizing extractant-impregnated graft-type particles.

PubMed

Uchiyama, Shoichiro; Sasaki, Takaaki; Ishihara, Ryo; Fujiwara, Kunio; Sugo, Takanobu; Umeno, Daisuke; Saito, Kyoichi

2018-01-19

An efficient method for rare metal recovery from environmental water and urban mines is in high demand. Toward rapid and high-resolution rare metal ion separation, a novel bis(2-ethylhexyl) phosphate (HDEHP)-impregnated graft-type particle as a filler for a chromatography column is proposed. To achieve rapid and high-resolution separation, a convection-flow-aided elution mode is required. The combination of 35 μm non-porous particles and a polymer-brush-rich particle structure minimizes the distance from metal ion binding sites to the convection flow in the column, resulting in minimized diffusional mass transfer resistance and the convection-flow-aided elution mode. The HDEHP-impregnated graft-type non-porous-particle-packed cartridge developed in this study exhibited a higher separation performance for model rare metals, neodymium (III) and dysprosium (III) ions, and a narrower peak at a higher linear velocity, than those of previous HDEHP-impregnated fiber-packed and commercially available Lewatit ® VP OC 1026-packed cartridges. Copyright © 2017 Elsevier B.V. All rights reserved.

High-resolution Identification and Separation of Living Cell Types by Multiple microRNA-responsive Synthetic mRNAs.

PubMed

Endo, Kei; Hayashi, Karin; Saito, Hirohide

2016-02-23

The precise identification and separation of living cell types is critical to both study cell function and prepare cells for medical applications. However, intracellular information to distinguish live cells remains largely inaccessible. Here, we develop a method for high-resolution identification and separation of cell types by quantifying multiple microRNA (miRNA) activities in live cell populations. We found that a set of miRNA-responsive, in vitro synthesized mRNAs identify a specific cell population as a sharp peak and clearly separate different cell types based on less than two-fold differences in miRNA activities. Increasing the number of miRNA-responsive mRNAs enhanced the capability for cell identification and separation, as we precisely and simultaneously distinguished different cell types with similar miRNA profiles. In addition, the set of synthetic mRNAs separated HeLa cells into subgroups, uncovering heterogeneity of the cells and the level of resolution achievable. Our method could identify target live cells and improve the efficiency of cell purification from heterogeneous populations.

What Geoscience Experts and Novices Look At, and What They See, When Viewing Data Visualizations

ERIC Educational Resources Information Center

Kastens, Kim A.; Shipley, Thomas F.; Boone, Alexander P.; Straccia, Frances

2016-01-01

This study examines how geoscience experts and novices make meaning from an iconic type of data visualization: shaded relief images of bathymetry and topography. Participants examined, described, and interpreted a global image, two high-resolution seafloor images, and 2 high-resolution continental images, while having their gaze direction…

Charting improvements in US registry HLA typing ambiguity using a typing resolution score.

PubMed

Paunić, Vanja; Gragert, Loren; Schneider, Joel; Müller, Carlheinz; Maiers, Martin

2016-07-01

Unrelated stem cell registries have been collecting HLA typing of volunteer bone marrow donors for over 25years. Donor selection for hematopoietic stem cell transplantation is based primarily on matching the alleles of donors and patients at five polymorphic HLA loci. As HLA typing technologies have continually advanced since the beginnings of stem cell transplantation, registries have accrued typings of varied HLA typing ambiguity. We present a new typing resolution score (TRS), based on the likelihood of self-match, that allows the systematic comparison of HLA typings across different methods, data sets and populations. We apply the TRS to chart improvement in HLA typing within the Be The Match Registry of the United States from the initiation of DNA-based HLA typing to the current state of high-resolution typing using next-generation sequencing technologies. In addition, we present a publicly available online tool for evaluation of any given HLA typing. This TRS objectively evaluates HLA typing methods and can help define standards for acceptable recruitment HLA typing. Copyright © 2016 American Society for Histocompatibility and Immunogenetics. Published by Elsevier Inc. All rights reserved.

High-resolution 3-T MRI of the triangular fibrocartilage complex in the wrist: injury pattern and MR features.

PubMed

Zhan, Huili; Zhang, Huibo; Bai, Rongjie; Qian, Zhanhua; Liu, Yue; Zhang, Heng; Yin, Yuming

2017-12-01

To investigate if using high-resolution 3-T MRI can identify additional injuries of the triangular fibrocartilage complex (TFCC) beyond the Palmer classification. Eighty-six patients with surgically proven TFCC injury were included in this study. All patients underwent high-resolution 3-T MRI of the injured wrist. The MR imaging features of TFCC were analyzed according to the Palmer classification. According to the Palmer classification, 69 patients could be classified as having Palmer injuries (52 had traumatic tears and 17 had degenerative tears). There were 17 patients whose injuries could not be classified according to the Palmer classification: 13 had volar or dorsal capsular TFC detachment and 4 had a horizontal tear of the articular disk. Using high-resolution 3-T MRI, we have not only found all the TFCC injuries described in the Palmer classification, additional injury types were found in this study, including horizontal tear of the TFC and capsular TFC detachment. We propose the modified Palmer classification and add the injury types that were not included in the original Palmer classification.

T85C polymorphisms of the dihydropyrimidine dehydrogenase gene detected in gastric cancer tissues by high-resolution melting curve analysis.

PubMed

Fang, Weijia; Xu, Nong; Jin, Dazhi; Chen, Yu; Chen, Xiaogang; Zheng, Yi; Shen, Hong; Yuan, Ying; Zheng, Shusen

2012-01-01

Dihydropyrimidine dehydrogenase is a key enzyme acting on the metabolic pathway of medications for gastric cancer. High-resolution melting curve technology, which was developed recently, can distinguish the wild-type dihydropyrimidine dehydrogenase gene from multiple polymorphisms by fluorescent quantitative polymerase chain reaction products in a direct and effective manner. T85C polymorphisms of dihydropyrimidine dehydrogenase in the peripheral blood of 112 Chinese gastric cancer patients were detected by real-time polymerase chain reaction combined with high-resolution melting curve technology. Primer design, along with the reaction system and conditions, was optimized based on the GenBank sequence. Seventy nine cases of wild-type (TT, [70.5%]), 29 cases of heterozygous (TC, [25.9%]), and 4 cases of homozygous mutant (CC, [3.6%]) were observed. The result was completely consistent with the results of the sequencing. Real-time polymerase chain reaction combined with high-resolution melting curve technology is a rapid, simple, reliable, direct-viewing, and convenient method for the detection and screening of polymorphisms.

Sub-25-nm laboratory x-ray microscopy using a compound Fresnel zone plate.

PubMed

von Hofsten, Olov; Bertilson, Michael; Reinspach, Julia; Holmberg, Anders; Hertz, Hans M; Vogt, Ulrich

2009-09-01

Improving the resolution in x-ray microscopes is of high priority to enable future applications in nanoscience. However, high-resolution zone-plate optics often have low efficiency, which makes implementation in laboratory microscopes difficult. We present a laboratory x-ray microscope based on a compound zone plate. The compound zone plate utilizes multiple diffraction orders to achieve high resolution while maintaining reasonable efficiency. We analyze the illumination conditions necessary for this type of optics in order to suppress stray light and demonstrate microscopic imaging resolving 25 nm features.

A method for generating high resolution satellite image time series

NASA Astrophysics Data System (ADS)

Guo, Tao

2014-10-01

There is an increasing demand for satellite remote sensing data with both high spatial and temporal resolution in many applications. But it still is a challenge to simultaneously improve spatial resolution and temporal frequency due to the technical limits of current satellite observation systems. To this end, much R&D efforts have been ongoing for years and lead to some successes roughly in two aspects, one includes super resolution, pan-sharpen etc. methods which can effectively enhance the spatial resolution and generate good visual effects, but hardly preserve spectral signatures and result in inadequate analytical value, on the other hand, time interpolation is a straight forward method to increase temporal frequency, however it increase little informative contents in fact. In this paper we presented a novel method to simulate high resolution time series data by combing low resolution time series data and a very small number of high resolution data only. Our method starts with a pair of high and low resolution data set, and then a spatial registration is done by introducing LDA model to map high and low resolution pixels correspondingly. Afterwards, temporal change information is captured through a comparison of low resolution time series data, and then projected onto the high resolution data plane and assigned to each high resolution pixel according to the predefined temporal change patterns of each type of ground objects. Finally the simulated high resolution data is generated. A preliminary experiment shows that our method can simulate a high resolution data with a reasonable accuracy. The contribution of our method is to enable timely monitoring of temporal changes through analysis of time sequence of low resolution images only, and usage of costly high resolution data can be reduces as much as possible, and it presents a highly effective way to build up an economically operational monitoring solution for agriculture, forest, land use investigation, environment and etc. applications.

Utilization of Short-Simulations for Tuning High-Resolution Climate Model

NASA Astrophysics Data System (ADS)

Lin, W.; Xie, S.; Ma, P. L.; Rasch, P. J.; Qian, Y.; Wan, H.; Ma, H. Y.; Klein, S. A.

2016-12-01

Many physical parameterizations in atmospheric models are sensitive to resolution. Tuning the models that involve a multitude of parameters at high resolution is computationally expensive, particularly when relying primarily on multi-year simulations. This work describes a complementary set of strategies for tuning high-resolution atmospheric models, using ensembles of short simulations to reduce the computational cost and elapsed time. Specifically, we utilize the hindcast approach developed through the DOE Cloud Associated Parameterization Testbed (CAPT) project for high-resolution model tuning, which is guided by a combination of short (< 10 days ) and longer ( 1 year) Perturbed Parameters Ensemble (PPE) simulations at low resolution to identify model feature sensitivity to parameter changes. The CAPT tests have been found to be effective in numerous previous studies in identifying model biases due to parameterized fast physics, and we demonstrate that it is also useful for tuning. After the most egregious errors are addressed through an initial "rough" tuning phase, longer simulations are performed to "hone in" on model features that evolve over longer timescales. We explore these strategies to tune the DOE ACME (Accelerated Climate Modeling for Energy) model. For the ACME model at 0.25° resolution, it is confirmed that, given the same parameters, major biases in global mean statistics and many spatial features are consistent between Atmospheric Model Intercomparison Project (AMIP)-type simulations and CAPT-type hindcasts, with just a small number of short-term simulations for the latter over the corresponding season. The use of CAPT hindcasts to find parameter choice for the reduction of large model biases dramatically improves the turnaround time for the tuning at high resolution. Improvement seen in CAPT hindcasts generally translates to improved AMIP-type simulations. An iterative CAPT-AMIP tuning approach is therefore adopted during each major tuning cycle, with the former to survey the likely responses and narrow the parameter space, and the latter to verify the results in climate context along with assessment in greater detail once an educated set of parameter choice is selected. Limitations on using short-term simulations for tuning climate model are also discussed.

High-Resolution FTIR Spectrum of the ν 5Band of HCOOD

NASA Astrophysics Data System (ADS)

Goh, K. L.; Ong, P. P.; Tan, T. L.; Teo, H. H.; Wang, W. F.

1998-10-01

The high-resolution Fourier transform infrared spectrum of HCOOD has been measured in the ν5region between 1120 and 1220 cm-1with a resolution of 0.004 cm-1. As expected for an in-plane vibrational fundamental mode, the ν5band is a hybrid band consisting of botha-type andb-type transitions. Using the Watson'sA-reduced Hamiltonian in theIrrepresentation, 1943 infrared transitions have been assigned and fitted to give 12 rovibrational constants for thev5= 1 state. The ν5band is primarilyAtype with a band center at 1177.09378 ± 0.00002 cm-1. It is found that ν5is slightly perturbed by the nearby 2ν7. About 90 perturbed transitions were identified.

VizieR Online Data Catalog: Magnetic early B-type stars. I. (Shultz+, 2018)

NASA Astrophysics Data System (ADS)

Shultz, M.; Wade, G. A.; Rivinius, Th.; Neiner, C.; Alecian, E.; Bohlender, D.; Monin, D.; Sikora, J.; Mimes Collaboration; Binamics Collaboration

2018-03-01

Longitudinal magnetic field measurements of early B-type stars derived from 1) least-squares deconvolution profiles extracted from high-resolution spectropolarimetric data (ESPaDOnS, Narval, HARPSpol), using masks consisting of metallic lines, metallic + He lines, individual chemical elements, as well as single-line H measurements; and 2) from single-line low-resolution spectropolarimetric observations with dimaPol. (3 data files).

MRI Superresolution Using Self-Similarity and Image Priors

PubMed Central

Manjón, José V.; Coupé, Pierrick; Buades, Antonio; Collins, D. Louis; Robles, Montserrat

2010-01-01

In Magnetic Resonance Imaging typical clinical settings, both low- and high-resolution images of different types are routinarily acquired. In some cases, the acquired low-resolution images have to be upsampled to match with other high-resolution images for posterior analysis or postprocessing such as registration or multimodal segmentation. However, classical interpolation techniques are not able to recover the high-frequency information lost during the acquisition process. In the present paper, a new superresolution method is proposed to reconstruct high-resolution images from the low-resolution ones using information from coplanar high resolution images acquired of the same subject. Furthermore, the reconstruction process is constrained to be physically plausible with the MR acquisition model that allows a meaningful interpretation of the results. Experiments on synthetic and real data are supplied to show the effectiveness of the proposed approach. A comparison with classical state-of-the-art interpolation techniques is presented to demonstrate the improved performance of the proposed methodology. PMID:21197094

Explicit and implicit compact high-resolution shock-capturing methods for multidimensional Euler equations 1: Formulation

NASA Technical Reports Server (NTRS)

Yee, H. C.

1995-01-01

Two classes of explicit compact high-resolution shock-capturing methods for the multidimensional compressible Euler equations for fluid dynamics are constructed. Some of these schemes can be fourth-order accurate away from discontinuities. For the semi-discrete case their shock-capturing properties are of the total variation diminishing (TVD), total variation bounded (TVB), total variation diminishing in the mean (TVDM), essentially nonoscillatory (ENO), or positive type of scheme for 1-D scalar hyperbolic conservation laws and are positive schemes in more than one dimension. These fourth-order schemes require the same grid stencil as their second-order non-compact cousins. One class does not require the standard matrix inversion or a special numerical boundary condition treatment associated with typical compact schemes. Due to the construction, these schemes can be viewed as approximations to genuinely multidimensional schemes in the sense that they might produce less distortion in spherical type shocks and are more accurate in vortex type flows than schemes based purely on one-dimensional extensions. However, one class has a more desirable high-resolution shock-capturing property and a smaller operation count in 3-D than the other class. The extension of these schemes to coupled nonlinear systems can be accomplished using the Roe approximate Riemann solver, the generalized Steger and Warming flux-vector splitting or the van Leer type flux-vector splitting. Modification to existing high-resolution second- or third-order non-compact shock-capturing computer codes is minimal. High-resolution shock-capturing properties can also be achieved via a variant of the second-order Lax-Friedrichs numerical flux without the use of Riemann solvers for coupled nonlinear systems with comparable operations count to their classical shock-capturing counterparts. The simplest extension to viscous flows can be achieved by using the standard fourth-order compact or non-compact formula for the viscous terms.

Generation of High Resolution Land Surface Parameters in the Community Land Model

NASA Astrophysics Data System (ADS)

Ke, Y.; Coleman, A. M.; Wigmosta, M. S.; Leung, L.; Huang, M.; Li, H.

2010-12-01

The Community Land Model (CLM) is the land surface model used for the Community Atmosphere Model (CAM) and the Community Climate System Model (CCSM). It examines the physical, chemical, and biological processes across a variety of spatial and temporal scales. Currently, efforts are being made to improve the spatial resolution of the CLM, in part, to represent finer scale hydrologic characteristics. Current land surface parameters of CLM4.0, in particular plant functional types (PFT) and leaf area index (LAI), are generated from MODIS and calculated at a 0.05 degree resolution. These MODIS-derived land surface parameters have also been aggregated to coarser resolutions (e.g., 0.5, 1.0 degrees). To evaluate the response of CLM across various spatial scales, higher spatial resolution land surface parameters need to be generated. In this study we examine the use of Landsat TM/ETM+ imagery and data fusion techniques for generating land surface parameters at a 1km resolution within the Pacific Northwest United States. . Land cover types and PFTs are classified based on Landsat multi-season spectral information, DEM, National Land Cover Database (NLCD) and the USDA-NASS Crop Data Layer (CDL). For each PFT, relationships between MOD15A2 high quality LAI values, Landsat-based vegetation indices, climate variables, terrain, and laser-altimeter derived vegetation height are used to generate monthly LAI values at a 30m resolution. The high-resolution PFT and LAI data are aggregated to create a 1km model grid resolution. An evaluation and comparison of CLM land surface response at both fine and moderate scale is presented.

Development and analytical characterization of a Grimm-type glow discharge ion source operated with high gas flow rates and coupled to a mass spectrometer with high mass resolution1

NASA Astrophysics Data System (ADS)

Beyer, Claus; Feldmann, Ingo; Gilmour, Dave; Hoffmann, Volker; Jakubowski, Norbert

2002-10-01

A Grimm-type glow discharge ion source has been developed and was coupled to a commercial inductively coupled plasma mass spectrometer (ICP-MS) with high mass resolution (Axiom, ThermoElemental, Winsford, UK) by exchanging the front plate of the ICP-MS interface system only. In addition to high discharge powers of up to 70 W, which are typical for a Grimm-type design, this source could be operated with relative high gas flow rates of up to 240 ml min -1. In combination with a high discharge voltage the signal intensities are reaching a constant level within the first 20 s after the discharge has started. An analytical characterization of this source is given utilizing a calibration using the steel standard reference material NIST 1261A-1265A. The sensitivity for the investigated elements measured with a resolution of 4000 is in the range of 500-6000 cps μg -1 g -1, and a relative standard deviation (R.S.D.) of the measured isotope relative to Fe of less than 8% for the major and minor components of the sample has been achieved. Limits of detection at ng g -1 levels could be obtained.

Large High Resolution Displays for Co-Located Collaborative Sensemaking: Display Usage and Territoriality

DOE Office of Scientific and Technical Information (OSTI.GOV)

Bradel, Lauren; Endert, Alexander; Koch, Kristen

2013-08-01

Large, high-resolution vertical displays carry the potential to increase the accuracy of collaborative sensemaking, given correctly designed visual analytics tools. From an exploratory user study using a fictional textual intelligence analysis task, we investigated how users interact with the display to construct spatial schemas and externalize information, as well as how they establish shared and private territories. We investigated the space management strategies of users partitioned by type of tool philosophy followed (visualization- or text-centric). We classified the types of territorial behavior exhibited in terms of how the users interacted with information on the display (integrated or independent workspaces). Next,more » we examined how territorial behavior impacted the common ground between the pairs of users. Finally, we offer design suggestions for building future co-located collaborative visual analytics tools specifically for use on large, high-resolution vertical displays.« less

Combining High Spatial Resolution Optical and LIDAR Data for Object-Based Image Classification

NASA Astrophysics Data System (ADS)

Li, R.; Zhang, T.; Geng, R.; Wang, L.

2018-04-01

In order to classify high spatial resolution images more accurately, in this research, a hierarchical rule-based object-based classification framework was developed based on a high-resolution image with airborne Light Detection and Ranging (LiDAR) data. The eCognition software is employed to conduct the whole process. In detail, firstly, the FBSP optimizer (Fuzzy-based Segmentation Parameter) is used to obtain the optimal scale parameters for different land cover types. Then, using the segmented regions as basic units, the classification rules for various land cover types are established according to the spectral, morphological and texture features extracted from the optical images, and the height feature from LiDAR respectively. Thirdly, the object classification results are evaluated by using the confusion matrix, overall accuracy and Kappa coefficients. As a result, a method using the combination of an aerial image and the airborne Lidar data shows higher accuracy.

Evaluation of Ion Torrent sequencing technology for rapid clinical human leucocyte antigen typing.

PubMed

Guerra, Sandra G; Chong, Winnie; Brown, Colin J; Navarrete, Cristina V

2018-06-05

The development of techniques to define the human leucocyte antigen (HLA) region has proven to be challenging due to its high level of polymorphism. Within a clinical laboratory, a technique for high-resolution HLA typing, which is rapid and cost effective is essential. NGS has provided a rapid, high-resolution HLA typing solution, which has reduced the number of HLA ambiguities seen with other typing methods. In this study, the One Lambda NXType NGS kit was tested on the Ion Torrent PGM platform. A total of 362 registry donors from four ethnic populations (Europeans, South Asians, Africans and Chinese) were NGS HLA typed across 9-loci (HLA-A, -B, -C, -DRB1,-DRB345 -DQB1 and -DPB1). Concordance rates of 91%-98% were obtained (for HLA-A, -B, -C, -DRB1, -DQB1 and -DPB1) when compared to historical PCR-SSO HLA types, and the identification of uncommon alleles such as A*24:07:01 and C*04:82 were observed. A turnaround time of four days was achieved for typing 44 samples. However, some limitations were observed; primer locations did not allow all ambiguities to be resolved for HLA Class II where Exon I and IV amplification are needed (HLA-DRB1*04:07:01/04:92, HLA-DRB1*09:01:02/*09:21 and HLA-DRB1*12:01:01/*12:10). This study has demonstrated high-resolution typing by NGS can be achieved in an acceptable turnaround time for a clinical laboratory; however, the Ion Torrent workflow has some technical limitations that should be addressed. © 2018 John Wiley & Sons Ltd.

A comparison of measured radiances from AIRS and HIRS across different cloud types

NASA Astrophysics Data System (ADS)

Schreier, M. M.; Kahn, B. H.; Staten, P.

2015-12-01

The observation of Earth's atmosphere with passive remote sensing instruments is ongoing for decades and resulting in a long-term global dataset. Two prominent examples are operational satellite platforms from the National Oceanic and Atmospheric Administration (NOAA) or research platforms like NASA's Earth Observing System (EOS). The observed spectral ranges of these observations are often similar among the different platforms, but have large differences when it comes to resolution, accuracy and quality control. Our approach is to combine different kinds of instruments at the pixel-scale to improve the characterization of infrared radiances. We focus on data from the High-resolution Infrared Radiation Sounder (HIRS) and compare the observations to radiances from the Atmospheric Infrared Sounder (AIRS) on Aqua. The high spectral resolution of AIRS is used to characterize and possibly recalibrate the observed radiances from HIRS. Our approach is unique in that we use additional information from other passive instruments on the same platforms including the Advanced Very High Resolution Radiometer (AVHRR) and the MODerate resolution Imaging Spectroradiometer (MODIS). We will present comparisons of radiances from HIRS and AIRS within different types of clouds that are determined from the imagers. In this way, we can analyze and select the most homogeneous conditions for radiance comparisons and a possible re-calibration of HIRS. We hope to achieve a cloud-type-dependent calibration and quality control for HIRS, which can be extrapolated into the past via inter-calibration of the different HIRS instruments beyond the time of AIRS.

High Spatiotemporal Resolution Prostate MRI

DTIC Science & Technology

2017-09-01

AWARD NUMBER: W81XWH-15-1-0341 TITLE: High Spatiotemporal Resolution Prostate MRI PRINCIPAL INVESTIGATOR: Stephen J. Riederer, Ph.D...views, opinions and/or findings contained in this report are those of the author(s) and should not be construed as an official Department of the Army...ADDRESS. 1. REPORT DATE September 2017 2. REPORT TYPE Annual 3. DATES COVERED 15 Aug 2016 - 14 Aug 2017 4. TITLE AND SUBTITLE High Spatiotemporal

High-Resolution 3T MR Imaging of the Triangular Fibrocartilage Complex

PubMed Central

von Borstel, Donald; Wang, Michael; Small, Kirstin; Nozaki, Taiki; Yoshioka, Hiroshi

2017-01-01

This study is intended as a review of 3Tesla (T) magnetic resonance (MR) imaging of the triangular fibrocartilage complex (TFCC). The recent advances in MR imaging, which includes high field strength magnets, multi-channel coils, and isotropic 3-dimensional (3D) sequences have enabled the visualization of precise TFCC anatomy with high spatial and contrast resolution. In addition to the routine wrist protocol, there are specific techniques used to optimize 3T imaging of the wrist; including driven equilibrium sequence (DRIVE), parallel imaging, and 3D imaging. The coil choice for 3T imaging of the wrist depends on a number of variables, and the proper coil design selection is critical for high-resolution wrist imaging with high signal and contrast-to-noise ratio. The TFCC is a complex structure and is composed of the articular disc (disc proper), the triangular ligament, the dorsal and volar radioulnar ligaments, the meniscus homologue, the ulnar collateral ligament (UCL), the extensor carpi ulnaris (ECU) tendon sheath, and the ulnolunate and ulnotriquetral ligaments. The Palmer classification categorizes TFCC lesions as traumatic (type 1) or degenerative (type 2). In this review article, we present clinical high-resolution MR images of normal TFCC anatomy and TFCC injuries with this classification system. PMID:27535592

High-Resolution 3T MR Imaging of the Triangular Fibrocartilage Complex.

PubMed

von Borstel, Donald; Wang, Michael; Small, Kirstin; Nozaki, Taiki; Yoshioka, Hiroshi

2017-01-10

This study is intended as a review of 3Tesla (T) magnetic resonance (MR) imaging of the triangular fibrocartilage complex (TFCC). The recent advances in MR imaging, which includes high field strength magnets, multi-channel coils, and isotropic 3-dimensional (3D) sequences have enabled the visualization of precise TFCC anatomy with high spatial and contrast resolution. In addition to the routine wrist protocol, there are specific techniques used to optimize 3T imaging of the wrist; including driven equilibrium sequence (DRIVE), parallel imaging, and 3D imaging. The coil choice for 3T imaging of the wrist depends on a number of variables, and the proper coil design selection is critical for high-resolution wrist imaging with high signal and contrast-to-noise ratio. The TFCC is a complex structure and is composed of the articular disc (disc proper), the triangular ligament, the dorsal and volar radioulnar ligaments, the meniscus homologue, the ulnar collateral ligament (UCL), the extensor carpi ulnaris (ECU) tendon sheath, and the ulnolunate and ulnotriquetral ligaments. The Palmer classification categorizes TFCC lesions as traumatic (type 1) or degenerative (type 2). In this review article, we present clinical high-resolution MR images of normal TFCC anatomy and TFCC injuries with this classification system.

Core Genome Multilocus Sequence Typing Scheme for High-Resolution Typing of Enterococcus faecium

PubMed Central

de Been, Mark; Pinholt, Mette; Top, Janetta; Bletz, Stefan; van Schaik, Willem; Brouwer, Ellen; Rogers, Malbert; Kraat, Yvette; Bonten, Marc; Corander, Jukka; Westh, Henrik; Harmsen, Dag

2015-01-01

Enterococcus faecium, a common inhabitant of the human gut, has emerged in the last 2 decades as an important multidrug-resistant nosocomial pathogen. Since the start of the 21st century, multilocus sequence typing (MLST) has been used to study the molecular epidemiology of E. faecium. However, due to the use of a small number of genes, the resolution of MLST is limited. Whole-genome sequencing (WGS) now allows for high-resolution tracing of outbreaks, but current WGS-based approaches lack standardization, rendering them less suitable for interlaboratory prospective surveillance. To overcome this limitation, we developed a core genome MLST (cgMLST) scheme for E. faecium. cgMLST transfers genome-wide single nucleotide polymorphism (SNP) diversity into a standardized and portable allele numbering system that is far less computationally intensive than SNP-based analysis of WGS data. The E. faecium cgMLST scheme was built using 40 genome sequences that represented the diversity of the species. The scheme consists of 1,423 cgMLST target genes. To test the performance of the scheme, we performed WGS analysis of 103 outbreak isolates from five different hospitals in the Netherlands, Denmark, and Germany. The cgMLST scheme performed well in distinguishing between epidemiologically related and unrelated isolates, even between those that had the same sequence type (ST), which denotes the higher discriminatory power of this cgMLST scheme over that of conventional MLST. We also show that in terms of resolution, the performance of the E. faecium cgMLST scheme is equivalent to that of an SNP-based approach. In conclusion, the cgMLST scheme developed in this study facilitates rapid, standardized, and high-resolution tracing of E. faecium outbreaks. PMID:26400782

Core Genome Multilocus Sequence Typing Scheme for High- Resolution Typing of Enterococcus faecium.

PubMed

de Been, Mark; Pinholt, Mette; Top, Janetta; Bletz, Stefan; Mellmann, Alexander; van Schaik, Willem; Brouwer, Ellen; Rogers, Malbert; Kraat, Yvette; Bonten, Marc; Corander, Jukka; Westh, Henrik; Harmsen, Dag; Willems, Rob J L

2015-12-01

Enterococcus faecium, a common inhabitant of the human gut, has emerged in the last 2 decades as an important multidrug-resistant nosocomial pathogen. Since the start of the 21st century, multilocus sequence typing (MLST) has been used to study the molecular epidemiology of E. faecium. However, due to the use of a small number of genes, the resolution of MLST is limited. Whole-genome sequencing (WGS) now allows for high-resolution tracing of outbreaks, but current WGS-based approaches lack standardization, rendering them less suitable for interlaboratory prospective surveillance. To overcome this limitation, we developed a core genome MLST (cgMLST) scheme for E. faecium. cgMLST transfers genome-wide single nucleotide polymorphism(SNP) diversity into a standardized and portable allele numbering system that is far less computationally intensive than SNP-based analysis of WGS data. The E. faecium cgMLST scheme was built using 40 genome sequences that represented the diversity of the species. The scheme consists of 1,423 cgMLST target genes. To test the performance of the scheme, we performed WGS analysis of 103 outbreak isolates from five different hospitals in the Netherlands, Denmark, and Germany. The cgMLST scheme performed well in distinguishing between epidemiologically related and unrelated isolates, even between those that had the same sequence type (ST), which denotes the higher discriminatory power of this cgMLST scheme over that of conventional MLST. We also show that in terms of resolution, the performance of the E. faecium cgMLST scheme is equivalent to that of an SNP-based approach. In conclusion, the cgMLST scheme developed in this study facilitates rapid, standardized, and high-resolution tracing of E. faecium outbreaks.

Study of hyperspectral characteristics of different types of flares and smoke candles

NASA Astrophysics Data System (ADS)

Farley, Vincent; Chamberland, Martin; Lagueux, Philippe; Kastek, Mariusz; Piatkowski, Tadeusz; Dulski, Rafal

2012-06-01

Modern infrared camouflage and countermeasure technologies used in the context of military operations have evolved rapidly over the last decade. Indeed, some infrared seekers and decoy/flares tend to have spectral sensitivity tailored to closely match the emission signatures of military vehicles (such as aircrafts, tanks) and reject other sources. Similarly, some candles (or smoke bombs) are developed to generate large area screens with very high absorption in the infrared. The Military University of Technology has conducted an intensive field campaign where various types of flares and smoke candles were deployed in different conditions and measured. The high spectral, spatial and temporal resolution acquisition of these thermodynamic events was recorded with the Telops Hyper-Cam. The Hyper-Cam enables simultaneous acquisition of spatial and spectral information at high resolutions in both domains. The ability to study combustion systems with high resolution, co-registered imagery and spectral data is made possible. This paper presents the test campaign concept and definition and the analysis of the recorded measurements.

Investigation of carbonates in the Sutter's Mill meteorite grains with hyperspectral infrared imaging micro-spectroscopy

NASA Astrophysics Data System (ADS)

Yesiltas, Mehmet

2018-04-01

Synchrotron-based high spatial resolution hyperspectral infrared imaging technique provides thousands of infrared spectra with high resolution, thus allowing us to acquire detailed spatial maps of chemical molecular structures for many grains in short times. Utilizing this technique, thousands of infrared spectra were analyzed at once instead of inspecting each spectrum separately. Sutter's Mill meteorite is a unique carbonaceous type meteorite with highly heterogeneous chemical composition. Multiple grains from the Sutter's Mill meteorite have been studied using this technique and the presence of both hydrous and anhydrous silicate minerals have been observed. It is observed that the carbonate mineralogy varies from simple to more complex carbonates even within a few microns in the meteorite grains. These variations, the type and distribution of calcite-like vs. dolomite-like carbonates are presented by means of hyperspectral FTIR imaging spectroscopy with high resolution. Various scenarios for the formation of different carbonate compositions in the Sutter's Mill parent body are discussed.

Defect detection on hardwood logs using high resolution three-dimensional laser scan data

Treesearch

Liya Thomas; Lamine Mili; Clifford A. Shaffer; Ed Thomas; Ed Thomas

2004-01-01

The location, type, and severity of external defects on hardwood logs and skills are the primary indicators of overall log quality and value. External defects provide hints about the internal log characteristics. Defect data would improve the sawyer's ability to process logs such that a higher valued product (lumber) is generated. Using a high-resolution laser log...

How low can you go? Changing the resolution of novel complex objects in visual working memory according to task demands

PubMed Central

Allon, Ayala S.; Balaban, Halely; Luria, Roy

2014-01-01

In three experiments we manipulated the resolution of novel complex objects in visual working memory (WM) by changing task demands. Previous studies that investigated the trade-off between quantity and resolution in visual WM yielded mixed results for simple familiar stimuli. We used the contralateral delay activity as an electrophysiological marker to directly track the deployment of visual WM resources while participants preformed a change-detection task. Across three experiments we presented the same novel complex items but changed the task demands. In Experiment 1 we induced a medium resolution task by using change trials in which a random polygon changed to a different type of polygon and replicated previous findings showing that novel complex objects are represented with higher resolution relative to simple familiar objects. In Experiment 2 we induced a low resolution task that required distinguishing between polygons and other types of stimulus categories, but we failed in finding a corresponding decrease in the resolution of the represented item. Finally, in Experiment 3 we induced a high resolution task that required discriminating between highly similar polygons with somewhat different contours. This time, we observed an increase in the item’s resolution. Our findings indicate that the resolution for novel complex objects can be increased but not decreased according to task demands, suggesting that minimal resolution is required in order to maintain these items in visual WM. These findings support studies claiming that capacity and resolution in visual WM reflect different mechanisms. PMID:24734026

Evaluation of High-Resolution Melting Curve Analysis of Ligation-Mediated Real-Time PCR, a Rapid Method for Epidemiological Typing of ESKAPE (Enterococcus faecium, Staphylococcus aureus, Klebsiella pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa, and Enterobacter Species) Pathogens

PubMed Central

Ryberg, Anna; Billström, Hanna; Hällgren, Anita; Nilsson, Lennart E.; Marklund, Britt-Inger; Olsson-Liljequist, Barbro; Schön, Thomas

2014-01-01

A single-tube method, ligation-mediated real-time PCR high-resolution melt analysis (LMqPCR HRMA), was modified for the rapid typing of Enterococcus faecium, Staphylococcus aureus, Klebsiella pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa, and Enterobacter spp. (ESKAPE) pathogens. A 97% agreement (60/62 isolates) was achieved in comparison to pulsed-field gel electrophoresis (PFGE) results, which indicates that LMqPCR HRMA is a rapid and accurate screening tool for monitoring nosocomial outbreaks. PMID:25232168

Orbitrap-MS and FT-ICR-MS of Free and Labile Organic Matter from Carbonaceous Chondrites

NASA Astrophysics Data System (ADS)

Orthous-Daunay, F.-R.; Thissen, R.; Vuitton, V.; Somogyi, A.; Mespoulede, M.; Beck, P.; Bonnet, J.-Y.; Dutuit, O.; Schmitt, B.; Quirico, E.

2011-03-01

We used two types of high-resolution FT-MS to analyze the free and labile organic matter in carbonaceous chondrites of type 1 and 2. The methanol extraction and laser desorption gave access to highly and poorly functionalized molecules respectively.

Low resolution spectroscopy of selected Algol systems

NASA Astrophysics Data System (ADS)

Devarapalli, Shanti Priya; Jagirdar, Rukmini; Parthasarathy, M.; Sahu, D. K.; Mohan, Vijay; Bhatt, B. C.; Thomas, Vineet S.

2018-04-01

The analysis of spectroscopic data for 30 Algol-type binaries is presented. All these systems are short period Algols having primaries with spectral types B and A. Dominant spectral lines were identified for the spectra collected and their equivalent widths were calculated. All the spectra were examined to understand presence of mass transfer, a disk or circumstellar matter and chromospheric emission. We also present first spectroscopic and period study for few Algols and conclude that high resolution spectra within and outside the primary minimum are needed for better understanding of these Algol type close binaries.

The influence of model grid resolution on estimation of national scale nitrogen deposition and exceedance of critical levels

NASA Astrophysics Data System (ADS)

Dore, A. J.; Kryza, M.; Hall, J. R.; Hallsworth, S.; Keller, V. J. D.; Vieno, M.; Sutton, M. A.

2011-12-01

The Fine Resolution Atmospheric Multi-pollutant Exchange model (FRAME) has been applied to model the spatial distribution of nitrogen deposition and air concentration over the UK at a 1 km spatial resolution. The modelled deposition and concentration data were gridded at resolutions of 1 km, 5 km and 50 km to test the sensitivity of calculations of the exceedance of critical loads for nitrogen deposition to the deposition data resolution. The modelled concentrations of NO2 were validated by comparison with measurements from the rural sites in the national monitoring network and were found to achieve better agreement with the high resolution 1 km data. High resolution plots were found to represent a more physically realistic distribution of nitrogen air concentrations and deposition resulting from use of 1 km resolution precipitation and emissions data as compared to 5 km resolution data. Summary statistics for national scale exceedance of the critical load for nitrogen deposition were not highly sensitive to the grid resolution of the deposition data but did show greater area exceedance with coarser grid resolution due to spatial averaging of high nitrogen deposition hot spots. Local scale deposition at individual Sites of Special Scientific Interest and high precipitation upland sites was sensitive to choice of grid resolution of deposition data. Use of high resolution data tended to generate lower deposition values in sink areas for nitrogen dry deposition (Sites of Scientific Interest) and higher values in high precipitation upland areas. In areas with generally low exceedance (Scotland) and for certain vegetation types (montane), the exceedance statistics were more sensitive to model data resolution.

The influence of model grid resolution on estimation of national scale nitrogen deposition and exceedance of critical loads

NASA Astrophysics Data System (ADS)

Dore, A. J.; Kryza, M.; Hall, J. R.; Hallsworth, S.; Keller, V. J. D.; Vieno, M.; Sutton, M. A.

2012-05-01

The Fine Resolution Atmospheric Multi-pollutant Exchange model (FRAME) was applied to model the spatial distribution of reactive nitrogen deposition and air concentration over the United Kingdom at a 1 km spatial resolution. The modelled deposition and concentration data were gridded at resolutions of 1 km, 5 km and 50 km to test the sensitivity of calculations of the exceedance of critical loads for nitrogen deposition to the deposition data resolution. The modelled concentrations of NO2 were validated by comparison with measurements from the rural sites in the national monitoring network and were found to achieve better agreement with the high resolution 1 km data. High resolution plots were found to represent a more physically realistic distribution of reactive nitrogen air concentrations and deposition resulting from use of 1 km resolution precipitation and emissions data as compared to 5 km resolution data. Summary statistics for national scale exceedance of the critical load for nitrogen deposition were not highly sensitive to the grid resolution of the deposition data but did show greater area exceedance with coarser grid resolution due to spatial averaging of high nitrogen deposition hot spots. Local scale deposition at individual Sites of Special Scientific Interest and high precipitation upland sites was sensitive to choice of grid resolution of deposition data. Use of high resolution data tended to generate lower deposition values in sink areas for nitrogen dry deposition (Sites of Scientific Interest) and higher values in high precipitation upland areas. In areas with generally low exceedance (Scotland) and for certain vegetation types (montane), the exceedance statistics were more sensitive to model data resolution.

High-resolution spectra of the 3.29 micron interstellar emission feature - A summary

NASA Technical Reports Server (NTRS)

Tokunaga, A. T.; Sellgren, K.; Smith, R. G.; Nagata, T.; Sakata, A.; Nakada, Y.

1991-01-01

High spectral resolution observations of the 3.29-micron interstellar emission feature show two types of profiles. Type 1 has a central wavelength of 3.289-micron and is observed in extended objects such as planetary nebulae and H II regions. Type 2 has a central wavelength of 3.296 microns and is observed around a small number of stellar sources. Type 2 has a full width at half-maximum of 0.020 micron; Type 1 has a broader FWHM, perhaps as much as 0.042 micron, but this is uncertain because of contamination by Pf(delta) emission. These profiles are tabulated for comparison to laboratory data. It is found that no proposed identification for the 3.29-micron emission feature definitely matches the observational spectra, although amorphous aromatic materials and heated polycyclic aromatic hydrocarbons tend to fit the best.

Recent applications of gas chromatography with high-resolution mass spectrometry.

PubMed

Špánik, Ivan; Machyňáková, Andrea

2018-01-01

Gas chromatography coupled to high-resolution mass spectrometry is a powerful analytical method that combines excellent separation power of gas chromatography with improved identification based on an accurate mass measurement. These features designate gas chromatography with high-resolution mass spectrometry as the first choice for identification and structure elucidation of unknown volatile and semi-volatile organic compounds. Gas chromatography with high-resolution mass spectrometry quantitative analyses was previously focused on the determination of dioxins and related compounds using magnetic sector type analyzers, a standing requirement of many international standards. The introduction of a quadrupole high-resolution time-of-flight mass analyzer broadened interest in this method and novel applications were developed, especially for multi-target screening purposes. This review is focused on the development and the most interesting applications of gas chromatography coupled to high-resolution mass spectrometry towards analysis of environmental matrices, biological fluids, and food safety since 2010. The main attention is paid to various approaches and applications of gas chromatography coupled to high-resolution mass spectrometry for non-target screening to identify contaminants and to characterize the chemical composition of environmental, food, and biological samples. The most interesting quantitative applications, where a significant contribution of gas chromatography with high-resolution mass spectrometry over the currently used methods is expected, will be discussed as well. © 2017 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Type-1 hepatorenal syndrome associated with infections in cirrhosis: natural history, outcome of kidney function, and survival.

PubMed

Barreto, Rogelio; Fagundes, Claudia; Guevara, Mónica; Solà, Elsa; Pereira, Gustavo; Rodríguez, Ezequiel; Graupera, Isabel; Martín-Llahí, Marta; Ariza, Xavier; Cárdenas, Andrés; Fernández, Javier; Rodés, Juan; Arroyo, Vicente; Ginès, Pere

2014-04-01

Type-1 hepatorenal syndrome (HRS) is a common complication of bacterial infections in cirrhosis, but its natural history remains undefined. To assess the outcome of kidney function and survival of patients with type-1 HRS associated with infections, 70 patients diagnosed during a 6-year period were evaluated prospectively. Main outcomes were no reversibility of type-1 HRS during treatment of the infection and 3-month survival. Forty-seven (67%) of the 70 patients had no reversibility of type-1 HRS during treatment of the infection. [Correction to previous sentence added March 10, 2014, after first online publication: "Twenty-three (33%)" was changed to "Forty-seven (67%)."] The main predictive factor of no reversibility of type-1 HRS was absence of infection resolution (no reversibility: 96% versus 48% in patients without and with resolution of the infection; P < 0.001). Independent predictive factors of no reversibility of type-1 HRS were age, high baseline serum bilirubin, nosocomial infection, and reduction in serum creatinine <0.3 mg/dL at day 3 of antibiotic treatment. No reversibility was also associated with severity of circulatory dysfunction, as indicated by more marked activity of the vasoconstrictor systems. In the whole series, 3-month probability of survival was only 21%. Factors associated with poor prognosis were baseline serum bilirubin, no reversibility of type-1 HRS, lack of resolution of the infection, and development of septic shock after diagnosis of type-1 HRS. Type-1 HRS associated with infections is not reversible in two-thirds of patients with treatment of infection only. No reversibility of type-1 HRS is associated with lack of resolution of the infection, age, high bilirubin, and no early improvement of kidney function and implies a poor prognosis. These results may help advance the management of patients with type-1 HRS associated with infections. © 2014 by the American Association for the Study of Liver Diseases.

Instrumentation in molecular imaging.

PubMed

Wells, R Glenn

2016-12-01

In vivo molecular imaging is a challenging task and no single type of imaging system provides an ideal solution. Nuclear medicine techniques like SPECT and PET provide excellent sensitivity but have poor spatial resolution. Optical imaging has excellent sensitivity and spatial resolution, but light photons interact strongly with tissues and so only small animals and targets near the surface can be accurately visualized. CT and MRI have exquisite spatial resolution, but greatly reduced sensitivity. To overcome the limitations of individual modalities, molecular imaging systems often combine individual cameras together, for example, merging nuclear medicine cameras with CT or MRI to allow the visualization of molecular processes with both high sensitivity and high spatial resolution.

A Modeling Approach to Global Land Surface Monitoring with Low Resolution Satellite Imaging

NASA Technical Reports Server (NTRS)

Hlavka, Christine A.; Dungan, Jennifer; Livingston, Gerry P.; Gore, Warren J. (Technical Monitor)

1998-01-01

The effects of changing land use/land cover on global climate and ecosystems due to greenhouse gas emissions and changing energy and nutrient exchange rates are being addressed by federal programs such as NASA's Mission to Planet Earth (MTPE) and by international efforts such as the International Geosphere-Biosphere Program (IGBP). The quantification of these effects depends on accurate estimates of the global extent of critical land cover types such as fire scars in tropical savannas and ponds in Arctic tundra. To address the requirement for accurate areal estimates, methods for producing regional to global maps with satellite imagery are being developed. The only practical way to produce maps over large regions of the globe is with data of coarse spatial resolution, such as Advanced Very High Resolution Radiometer (AVHRR) weather satellite imagery at 1.1 km resolution or European Remote-Sensing Satellite (ERS) radar imagery at 100 m resolution. The accuracy of pixel counts as areal estimates is in doubt, especially for highly fragmented cover types such as fire scars and ponds. Efforts to improve areal estimates from coarse resolution maps have involved regression of apparent area from coarse data versus that from fine resolution in sample areas, but it has proven difficult to acquire sufficient fine scale data to develop the regression. A method for computing accurate estimates from coarse resolution maps using little or no fine data is therefore needed.

Production of high-resolution forest-ecosite maps based on model predictions of soil moisture and nutrient regimes over a large forested area.

PubMed

Yang, Qi; Meng, Fan-Rui; Bourque, Charles P-A; Zhao, Zhengyong

2017-09-08

Forest ecosite reflects the local site conditions that are meaningful to forest productivity as well as basic ecological functions. Field assessments of vegetation and soil types are often used to identify forest ecosites. However, the production of high-resolution ecosite maps for large areas from interpolating field data is difficult because of high spatial variation and associated costs and time requirements. Indices of soil moisture and nutrient regimes (i.e., SMR and SNR) introduced in this study reflect the combined effects of biogeochemical and topographic factors on forest growth. The objective of this research is to present a method for creating high-resolution forest ecosite maps based on computer-generated predictions of SMR and SNR for an area in Atlantic Canada covering about 4.3 × 10 6 hectares (ha) of forestland. Field data from 1,507 forest ecosystem classification plots were used to assess the accuracy of the ecosite maps produced. Using model predictions of SMR and SNR alone, ecosite maps were 61 and 59% correct in identifying 10 Acadian- and Maritime-Boreal-region ecosite types, respectively. This method provides an operational framework for the production of high-resolution maps of forest ecosites over large areas without the need for data from expensive, supplementary field surveys.

Characterization of 1.2×1.2 mm2 silicon photomultipliers with Ce:LYSO, Ce:GAGG, and Pr:LuAG scintillation crystals as detector modules for positron emission tomography

NASA Astrophysics Data System (ADS)

Omidvari, N.; Sharma, R.; Ganka, T. R.; Schneider, F. R.; Paul, S.; Ziegler, S. I.

2017-04-01

The design of a positron emission tomography (PET) scanner is specially challenging since it should not compromise high spatial resolution, high sensitivity, high count-rate capability, and good energy and time resolution. The geometrical design of the system alongside the characteristics of the individual PET detector modules contributes to the overall performance of the scanner. The detector performance is mainly influenced by the characteristics of the photo-detector and the scintillation crystal. Although silicon photomultipliers (SiPMs) have already proven to be promising photo-detectors for PET, their performance is highly influenced by micro-cell structure and production technology. Therefore, five types of SiPMs produced by KETEK with an active area size of 1.2 × 1.2 mm2 were characterized in this study. The SiPMs differed in the production technology and had micro-cell sizes of 25, 50, 75, and 100 μm. Performance of the SiPMs was evaluated in terms of their breakdown voltage, temperature sensitivity, dark count rate, and correlated noise probability. Subsequently, energy resolution and coincidence time resolution (CTR) of the SiPMs were measured with five types of crystals, including two Ce:LYSO, two Ce:GAGG, and one Pr:LuAG. Two crystals with a geometry of 1.5 × 1.5 × 6 mm3 were available from each type. The best CTR achieved was ~ 240 ps, which was obtained with the Ce:LYSO crystals coupled to the 50 μm SiPM produced with the trench technology. The best energy resolution for the 511 keV photo-peak was ~ 11% and was obtained with the same SiPM coupled to the Ce:GAGG crystals.

High-resolution photo-mosaic time-series imagery for monitoring human use of an artificial reef.

PubMed

Wood, Georgina; Lynch, Tim P; Devine, Carlie; Keller, Krystle; Figueira, Will

2016-10-01

Successful marine management relies on understanding patterns of human use. However, obtaining data can be difficult and expensive given the widespread and variable nature of activities conducted. Remote camera systems are increasingly used to overcome cost limitations of conventional labour-intensive methods. Still, most systems face trade-offs between the spatial extent and resolution over which data are obtained, limiting their application. We trialed a novel methodology, CSIRO Ruggedized Autonomous Gigapixel System (CRAGS), for time series of high-resolution photo-mosaic (HRPM) imagery to estimate fine-scale metrics of human activity at an artificial reef located 1.3 km from shore. We compared estimates obtained using the novel system to those produced with a web camera that concurrently monitored the site. We evaluated the effect of day type (weekday/weekend) and time of day on each of the systems and compared to estimates obtained from binocular observations. In general, both systems delivered similar estimates for the number of boats observed and to those obtained by binocular counts; these results were also unaffected by the type of day (weekend vs. weekday). CRAGS was able to determine additional information about the user type and party size that was not possible with the lower resolution webcam system. However, there was an effect of time of day as CRAGS suffered from poor image quality in early morning conditions as a result of fixed camera settings. Our field study provides proof of concept of use of this new cost-effective monitoring tool for the remote collection of high-resolution large-extent data on patterns of human use at high temporal frequency.

Applications and Innovations for Use of High Definition and High Resolution Digital Motion Imagery in Space Operations

NASA Technical Reports Server (NTRS)

Grubbs, Rodney

2016-01-01

The first live High Definition Television (HDTV) from a spacecraft was in November, 2006, nearly ten years before the 2016 SpaceOps Conference. Much has changed since then. Now, live HDTV from the International Space Station (ISS) is routine. HDTV cameras stream live video views of the Earth from the exterior of the ISS every day on UStream, and HDTV has even flown around the Moon on a Japanese Space Agency spacecraft. A great deal has been learned about the operations applicability of HDTV and high resolution imagery since that first live broadcast. This paper will discuss the current state of real-time and file based HDTV and higher resolution video for space operations. A potential roadmap will be provided for further development and innovations of high-resolution digital motion imagery, including gaps in technology enablers, especially for deep space and unmanned missions. Specific topics to be covered in the paper will include: An update on radiation tolerance and performance of various camera types and sensors and ramifications on the future applicability of these types of cameras for space operations; Practical experience with downlinking very large imagery files with breaks in link coverage; Ramifications of larger camera resolutions like Ultra-High Definition, 6,000 [pixels] and 8,000 [pixels] in space applications; Enabling technologies such as the High Efficiency Video Codec, Bundle Streaming Delay Tolerant Networking, Optical Communications and Bayer Pattern Sensors and other similar innovations; Likely future operations scenarios for deep space missions with extreme latency and intermittent communications links.

Fabrication of high-resolution x-ray diffractive optics at King's College London

NASA Astrophysics Data System (ADS)

Charalambous, Pambos S.; Anastasi, Peter A. F.; Burge, Ronald E.; Popova, Katia

1995-09-01

The fabrication of high resolution x-ray diffractive optics, and Fresnel zone plates (ZPs) in particular, is a very demanding multifaceted technological task. The commissioning of more (and brighter) synchrotron radiation sources, has increased the number of x-ray imaging beam lines world wide. The availability of cheaper and more effective laboratory x-ray sources, has further increased the number of laboratories involved in x-ray imaging. The result is an ever increasing demand for x-ray optics with a very wide range of specifications, reflecting the particular type of x-ray imaging performed at different laboratories. We have been involved in all aspects of high resolution nanofabrication for a number of years, and we have explored many different methods of lithography, which, although unorthodox, open up possibilities, and increase our flexibility for the fabrication of different diffractive optical elements, as well as other types of nanostructures. The availability of brighter x-ray sources, means that the diffraction efficiency of the ZPs is becoming of secondary importance, a trend which will continue in the future. Resolution, however, is important and will always remain so. Resolution is directly related to the accuracy af pattern generation, as well as the ability to draw fine lines. This is the area towards which we have directed most of our efforts so far.

RBS/C, HRTEM and HRXRD study of damage accumulation in irradiated SrTiO3

DOE Office of Scientific and Technical Information (OSTI.GOV)

Jagielski, Jacek; Jozwik, Przemyslaw A.; Jozwik Biala, Iwona

2013-05-14

Damage accumulation in argon-irradiated SrTiO3 single crystals has been studied by using combination of Rutherford Backscattering/Channeling (RBS/C), High Resolution Transmission Electron Microscopy (HRTEM) and High Resolution X-Ray Diffraction (HRXRD) techniques. The RBS/C spectra were fitted using McChasy, a Monte Carlo simulation code allowing the quantitative analysis of amorphous-like and dislocation-like types of defects. The results were interpreted by using a Multi-Step Damage Accumulation model which assumes, that the damage accumulation occurs in a series of structural transformations, the defect transformations are triggered by a stress caused by formation of a free volume in the irradiated crystal. This assumption has beenmore » confirmed by High Resolution Transmission Electron Microscopy and High Resolution X-Ray Diffraction analysis.« less

A high-resolution cattle CNV map by population-scale genome sequencing

USDA-ARS?s Scientific Manuscript database

Copy Number Variations (CNVs) are common genomic structural variations that have been linked to human diseases and phenotypic traits. CNVs represent an important type of genetic variation among cattle breeds and even individual animals; however, only low-resolution maps of cattle CNVs currently exis...

A high time and spatial resolution MRPC designed for muon tomography

NASA Astrophysics Data System (ADS)

Shi, L.; Wang, Y.; Huang, X.; Wang, X.; Zhu, W.; Li, Y.; Cheng, J.

2014-12-01

A prototype of cosmic muon scattering tomography system has been set up in Tsinghua University in Beijing. Multi-gap Resistive Plate Chamber (MRPC) is used in the system to get the muon tracks. Compared with other detectors, MRPC can not only provide the track but also the Time of Flight (ToF) between two detectors which can estimate the energy of particles. To get a more accurate track and higher efficiency of the tomography system, a new type of high time and two-dimensional spatial resolution MRPC has been developed. A series of experiments have been done to measure the efficiency, time resolution and spatial resolution. The results show that the efficiency can reach 95% and its time resolution is around 65 ps. The cluster size is around 4 and the spatial resolution can reach 200 μ m.

High resolution extensometer based on optical encoder for measurement of small landslide displacements

NASA Astrophysics Data System (ADS)

Afandi, M. I.; Adinanta, H.; Setiono, A.; Qomaruddin; Widiyatmoko, B.

2018-03-01

There are many ways to measure landslide displacement using sensors such as multi-turn potentiometer, fiber optic strain sensor, GPS, geodetic measurement, ground penetrating radar, etc. The proposed way is to use an optical encoder that produces pulse signal with high stability of measurement resolution despite voltage source instability. The landslide measurement using extensometer based on optical encoder has the ability of high resolution for wide range measurement and for a long period of time. The type of incremental optical encoder provides information about the pulse and direction of a rotating shaft by producing quadrature square wave cycle per increment of shaft movement. The result of measurement using 2,000 pulses per resolution of optical encoder has been obtained. Resolution of extensometer is 36 μm with speed limit of about 3.6 cm/s. System test in hazard landslide area has been carried out with good reliability for small landslide displacement monitoring.

Evaluation of high-resolution melting curve analysis of ligation-mediated real-time PCR, a rapid method for epidemiological typing of ESKAPE (Enterococcus faecium, Staphylococcus aureus, Klebsiella pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa, and Enterobacter Species) pathogens.

PubMed

Woksepp, Hanna; Ryberg, Anna; Billström, Hanna; Hällgren, Anita; Nilsson, Lennart E; Marklund, Britt-Inger; Olsson-Liljequist, Barbro; Schön, Thomas

2014-12-01

A single-tube method, ligation-mediated real-time PCR high-resolution melt analysis (LMqPCR HRMA), was modified for the rapid typing of Enterococcus faecium, Staphylococcus aureus, Klebsiella pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa, and Enterobacter spp. (ESKAPE) pathogens. A 97% agreement (60/62 isolates) was achieved in comparison to pulsed-field gel electrophoresis (PFGE) results, which indicates that LMqPCR HRMA is a rapid and accurate screening tool for monitoring nosocomial outbreaks. Copyright © 2014, American Society for Microbiology. All Rights Reserved.

High resolution tsunami modelling for the evaluation of potential risk areas in Setúbal (Portugal)

NASA Astrophysics Data System (ADS)

Ribeiro, J.; Silva, A.; Leitão, P.

2011-08-01

The use of high resolution hydrodynamic modelling to simulate the potential effects of tsunami events can provide relevant information about the most probable inundation areas. Moreover, the consideration of complementary data such as the type of buildings, location of priority equipment, type of roads, enables mapping of the most vulnerable zones, computing of the expected damage on man-made structures, constrain of the definition of rescue areas and escape routes, adaptation of emergency plans and proper evaluation of the vulnerability associated with different areas and/or equipment. Such an approach was used to evaluate the specific risks associated with a potential occurrence of a tsunami event in the region of Setúbal (Portugal), which was one of the areas most seriously affected by the 1755 tsunami. In order to perform an evaluation of the hazard associated with the occurrence of a similar event, high resolution wave propagation simulations were performed considering different potential earthquake sources with different magnitudes. Based on these simulations, detailed inundation maps associated with the different events were produced. These results were combined with the available information on the vulnerability of the local infrastructures (building types, roads and streets characteristics, priority buildings) in order to impose restrictions in the production of high-scale potential damage maps, escape routes and emergency routes maps.

Continuous Beam Steering From a Segmented Liquid Crystal Optical Phased Array

NASA Technical Reports Server (NTRS)

Titus, Charles M.; Pouch, John; Nguyen, Hung; Miranda, Felix; Bos, Philip J.

2002-01-01

Optical communications to and from deep space probes will require beams possessing divergence on the order of a microradian, and must be steered with sub-microradian precision. Segmented liquid crystal spatial phase modulators, a type of optical phased array, are considered for this ultra-high resolution beam steering. It is shown here that in an ideal device of this type, there are ultimately no restrictions on the angular resolution. Computer simulations are used to obtain that result, and to analyze the influence of beam truncation and substrate flatness on the performance of this type of device.

Continuous Beam Steering From A Segmented Liquid Crystal Optical Phased Array

NASA Technical Reports Server (NTRS)

Pouch, John; Nguyen, Hung; Miranda, Felix; Titus, Charles M.; Bos, Philip J.

2002-01-01

Optical communications to and from deep space probes will require beams possessing divergence on the order of a microradian, and must be steered with sub-microradian precision. Segmented liquid crystal spatial phase modulators, a type of optical phased array, are considered for this ultra-high resolution beam steering. It is shown here that in an ideal device of this type, there are ultimately no restrictions on the angular resolution. Computer simulations are used to obtain that result, and to analyze the influence of beam truncation and substrate flatness on the performance of this type of device.

High-pressure, High-temperature Deformation Experiment Using the New Generation Griggs-type Apparatus

PubMed Central

Précigout, Jacques; Stünitz, Holger; Pinquier, Yves; Champallier, Rémi; Schubnel, Alexandre

2018-01-01

In order to address geological processes at great depths, rock deformation should ideally be tested at high pressure (> 0.5 GPa) and high temperature (> 300 °C). However, because of the low stress resolution of current solid-pressure-medium apparatuses, high-resolution measurements are today restricted to low-pressure deformation experiments in the gas-pressure-medium apparatus. A new generation of solid-medium piston-cylinder ("Griggs-type") apparatus is here described. Able to perform high-pressure deformation experiments up to 5 GPa and designed to adapt an internal load cell, such a new apparatus offers the potential to establish a technological basis for high-pressure rheology. This paper provides video-based detailed documentation of the procedure (using the "conventional" solid-salt assembly) to perform high-pressure, high-temperature experiments with the newly designed Griggs-type apparatus. A representative result of a Carrara marble sample deformed at 700 °C, 1.5 GPa and 10-5 s-1 with the new press is also given. The related stress-time curve illustrates all steps of a Griggs-type experiment, from increasing pressure and temperature to sample quenching when deformation is stopped. Together with future developments, the critical steps and limitations of the Griggs apparatus are then discussed. PMID:29683444

Rapid, scalable and highly automated HLA genotyping using next-generation sequencing: a transition from research to diagnostics

PubMed Central

2013-01-01

Background Human leukocyte antigen matching at allelic resolution is proven clinically significant in hematopoietic stem cell transplantation, lowering the risk of graft-versus-host disease and mortality. However, due to the ever growing HLA allele database, tissue typing laboratories face substantial challenges. In light of the complexity and the high degree of allelic diversity, it has become increasingly difficult to define the classical transplantation antigens at high-resolution by using well-tried methods. Thus, next-generation sequencing is entering into diagnostic laboratories at the perfect time and serving as a promising tool to overcome intrinsic HLA typing problems. Therefore, we have developed and validated a scalable automated HLA class I and class II typing approach suitable for diagnostic use. Results A validation panel of 173 clinical and proficiency testing samples was analysed, demonstrating 100% concordance to the reference method. From a total of 1,273 loci we were able to generate 1,241 (97.3%) initial successful typings. The mean ambiguity reduction for the analysed loci was 93.5%. Allele assignment including intronic sequences showed an improved resolution (99.2%) of non-expressed HLA alleles. Conclusion We provide a powerful HLA typing protocol offering a short turnaround time of only two days, a fully integrated workflow and most importantly a high degree of typing reliability. The presented automated assay is flexible and can be scaled by specific primer compilations and the use of different 454 sequencing systems. The workflow was successfully validated according to the policies of the European Federation for Immunogenetics. Next-generation sequencing seems to become one of the new methods in the field of Histocompatibility. PMID:23557197

Formation routes and structural details of the CaF1 layer on Si(111) from high-resolution noncontact atomic force microscopy data

NASA Astrophysics Data System (ADS)

Rahe, Philipp; Smith, Emily F.; Wollschläger, Joachim; Moriarty, Philip J.

2018-03-01

We investigate the CaF1/Si (111 ) interface using a combination of high-resolution scanning tunneling and noncontact atomic force microscopy operated at cryogenic temperature as well as x-ray photoelectron spectroscopy. Submonolayer CaF1 films grown at substrate temperatures between 550 and 600 ∘C on Si (111 ) surfaces reveal the existence of two island types that are distinguished by their edge topology, nucleation position, measured height, and inner defect structure. Our data suggest a growth model where the two island types are the result of two reaction pathways during CaF1 interface formation. A key difference between these two pathways is identified to arise from the excess species during the growth process, which can be either fluorine or silicon. Structural details as a result of this difference are identified by means of high-resolution noncontact atomic force microscopy and add insights into the growth mode of this heteroepitaxial insulator-on-semiconductor system.

Capability of AVHRR data in discriminating rangeland cover mixtures

USGS Publications Warehouse

Senay, Gabriel B.; Elliott, R.L.

2002-01-01

A combination of high temporal resolution Advanced Very High Resolution Radiometer (AVHRR) data and high spatial information Map Information Analysis and Display System (MIADS) landuse/landcover data from the United States Department of Agriculture-Natural Resources Conservation Service (USDA-NRCS) were used to investigate the feasibility of using the combined dataset for regional evapotranspiration (ET) studies. It was shown that the biweekly maximum Normalized Difference Vegetation Index (NDVI) composite AVHRR data were capable of discriminating rangelands with different types of trees and shrubs species. AVHRR data also showed a potential to distinguish canopy cover differences within a mix of similar species. The combination of MIADS data and AVHRR data can be used to study temporal dynamics of various cover types for use in regional ET estimates.

What have we learned from the German consortium project STORM aiming at high-resolution climate simulations?

NASA Astrophysics Data System (ADS)

von Storch, Jin-Song

2014-05-01

The German consortium STORM was built to explore high-resolution climate simulations using the high-performance computer stored at the German Climate Computer Center (DKRZ). One of the primary goals is to quantify the effect of unresolved (and parametrized) processes on climate sensitivity. We use ECHAM6/MPIOM, the coupled atmosphere-ocean model developed at the Max-Planck Institute for Meteorology. The resolution is T255L95 for the atmosphere and 1/10 degree and 80 vertical levels for the ocean. We discuss results of stand-alone runs, i.e. the ocean-only simulation driven by the NCEP/NCAR renalaysis and the atmosphere-only AMIP-type of simulation. Increasing resolution leads to a redistribution of biases, even though some improvements, both in the atmosphere and in the ocean, can clearly be attributed to the increase in resolution. We represent also new insights on ocean meso-scale eddies, in particular their effects on the ocean's energetics. Finally, we discuss the status and problems of the coupled high-resolution runs.

X-ray diagnostics of massive star winds

NASA Astrophysics Data System (ADS)

Oskinova, L. M.; Ignace, R.; Huenemoerder, D. P.

2017-11-01

Observations with powerful X-ray telescopes, such as XMM-Newton and Chandra, significantly advance our understanding of massive stars. Nearly all early-type stars are X-ray sources. Studies of their X-ray emission provide important diagnostics of stellar winds. High-resolution X-ray spectra of O-type stars are well explained when stellar wind clumping is taking into account, providing further support to a modern picture of stellar winds as non-stationary, inhomogeneous outflows. X-ray variability is detected from such winds, on time scales likely associated with stellar rotation. High-resolution X-ray spectroscopy indicates that the winds of late O-type stars are predominantly in a hot phase. Consequently, X-rays provide the best observational window to study these winds. X-ray spectroscopy of evolved, Wolf-Rayet type, stars allows to probe their powerful metal enhanced winds, while the mechanisms responsible for the X-ray emission of these stars are not yet understood.

High Resolution Numerical Simulations of Primary Atomization in Diesel Sprays with Single Component Reference Fuels

DTIC Science & Technology

2015-09-01

NC. 14. ABSTRACT A high-resolution numerical simulation of jet breakup and spray formation from a complex diesel fuel injector at diesel engine... diesel fuel injector at diesel engine type conditions has been performed. A full understanding of the primary atomization process in diesel fuel... diesel liquid sprays the complexity is further compounded by the physical attributes present including nozzle turbulence, large density ratios

Assessment of a high-resolution central scheme for the solution of the relativistic hydrodynamics equations

NASA Astrophysics Data System (ADS)

Lucas-Serrano, A.; Font, J. A.; Ibáñez, J. M.; Martí, J. M.

2004-12-01

We assess the suitability of a recent high-resolution central scheme developed by \\cite{kurganov} for the solution of the relativistic hydrodynamic equations. The novelty of this approach relies on the absence of Riemann solvers in the solution procedure. The computations we present are performed in one and two spatial dimensions in Minkowski spacetime. Standard numerical experiments such as shock tubes and the relativistic flat-faced step test are performed. As an astrophysical application the article includes two-dimensional simulations of the propagation of relativistic jets using both Cartesian and cylindrical coordinates. The simulations reported clearly show the capabilities of the numerical scheme of yielding satisfactory results, with an accuracy comparable to that obtained by the so-called high-resolution shock-capturing schemes based upon Riemann solvers (Godunov-type schemes), even well inside the ultrarelativistic regime. Such a central scheme can be straightforwardly applied to hyperbolic systems of conservation laws for which the characteristic structure is not explicitly known, or in cases where a numerical computation of the exact solution of the Riemann problem is prohibitively expensive. Finally, we present comparisons with results obtained using various Godunov-type schemes as well as with those obtained using other high-resolution central schemes which have recently been reported in the literature.

Forest cover type analysis of New England forests using innovative WorldView-2 imagery

NASA Astrophysics Data System (ADS)

Kovacs, Jenna M.

For many years, remote sensing has been used to generate land cover type maps to create a visual representation of what is occurring on the ground. One significant use of remote sensing is the identification of forest cover types. New England forests are notorious for their especially complex forest structure and as a result have been, and continue to be, a challenge when classifying forest cover types. To most accurately depict forest cover types occurring on the ground, it is essential to utilize image data that have a suitable combination of both spectral and spatial resolution. The WorldView-2 (WV2) commercial satellite, launched in 2009, is the first of its kind, having both high spectral and spatial resolutions. WV2 records eight bands of multispectral imagery, four more than the usual high spatial resolution sensors, and has a pixel size of 1.85 meters at the nadir. These additional bands have the potential to improve classification detail and classification accuracy of forest cover type maps. For this reason, WV2 imagery was utilized on its own, and in combination with Landsat 5 TM (LS5) multispectral imagery, to evaluate whether these image data could more accurately classify forest cover types. In keeping with recent developments in image analysis, an Object-Based Image Analysis (OBIA) approach was used to segment images of Pawtuckaway State Park and nearby private lands, an area representative of the typical complex forest structure found in the New England region. A Classification and Regression Tree (CART) analysis was then used to classify image segments at two levels of classification detail. Accuracies for each forest cover type map produced were generated using traditional and area-based error matrices, and additional standard accuracy measures (i.e., KAPPA) were generated. The results from this study show that there is value in analyzing imagery with both high spectral and spatial resolutions, and that WV2's new and innovative bands can be useful for the classification of complex forest structures.

Mid-infrared interferometry of AGNs: A statistical view into the dusty nuclear environment of the Seyfert Galaxies.

NASA Astrophysics Data System (ADS)

Lopez-Gonzaga, N.

2015-09-01

The high resolution achieved by the instrument MIDI at the VLTI allowed to obtain more detail information about the geometry and structure of the nuclear mid-infrared emission of AGNs, but due to the lack of real images, the interpretation of the results is not an easy task. To profit more from the high resolution data, we developed a statistical tool that allows interpret these data using clumpy torus models. A statistical approach is needed to overcome effects such as, the randomness in the position of the clouds and the uncertainty of the true position angle on the sky. Our results, obtained by studying the mid-infrared emission at the highest resolution currently available, suggest that the dusty environment of Type I objects is formed by a lower number of clouds than Type II objects.

The role of digital sample information within the digital geoscience infrastructure: a pragmatic approach

NASA Astrophysics Data System (ADS)

Howe, Michael

2014-05-01

Much of the digital geological information on the composition, properties and dynamics of the subsurface is based ultimately on physical samples, many of which are archived to provide a basis for the information. Online metadata catalogues of these collections have now been available for many years. Many of these are institutional and tightly focussed, with UK examples including the British Geological Survey's (BGS) palaeontological samples database, PalaeoSaurus (http://www.bgs.ac.uk/palaeosaurus/), and mineralogical and petrological sample database, Britrocks (http://www.bgs.ac.uk/data/britrocks.html) . There are now a growing number of international sample metadata databases, including The Palaeobiology Database (http://paleobiodb.org/) and SESAR, the IGSN (International Geo Sample Number) database (http://www.geosamples.org/catalogsearch/ ). More recently the emphasis has moved beyond metadata (locality, identification, age, citations, etc) to digital imagery, with the intention of providing the user with at least enough information to determine whether viewing the sample would be worthwhile. Recent BGS examples include high resolution (e.g. 7216 x 5412 pixel) hydrocarbon well core images (http://www.bgs.ac.uk/data/offshoreWells/wells.cfc?method=searchWells) , high resolution rock thin section images (e.g. http://www.largeimages.bgs.ac.uk/iip/britrocks.html?id=290000/291739 ) and building stone images (http://geoscenic.bgs.ac.uk/asset-bank/action/browseItems?categoryId=1547&categoryTypeId=1) . This has been developed further with high resolution stereo images. The Jisc funded GB3D type fossils online project delivers these as red-cyan anaglyphs (http://www.3d-fossils.ac.uk/). More innovatively, the GB3D type fossils project has laser scanned several thousand type fossils and the resulting 3d-digital models are now being delivered through the online portal. Importantly, this project also represents collaboration between the BGS, Oxford and Cambridge Universities, the National Museums of Wales, and numerous other national, local and regional museums. The lack of currently accepted international standards and infrastructures for the delivery of high resolution images and 3d-digital models has necessitated the BGS in developing or selecting its own. Most high resolution images have been delivered using the JPEG 2000 format because of its quality and speed. Digital models have been made available in both .PLY and .OBJ format because of their respective efficient file size, and flexibility. Consideration must now be given to European and international standards and infrastructures for the delivery of high resolution images and 3d-digital models.

Temporal and spatial scaling impacts on extreme precipitation

NASA Astrophysics Data System (ADS)

Eggert, B.; Berg, P.; Haerter, J. O.; Jacob, D.; Moseley, C.

2015-01-01

Both in the current climate and in the light of climate change, understanding of the causes and risk of precipitation extremes is essential for protection of human life and adequate design of infrastructure. Precipitation extreme events depend qualitatively on the temporal and spatial scales at which they are measured, in part due to the distinct types of rain formation processes that dominate extremes at different scales. To capture these differences, we first filter large datasets of high-resolution radar measurements over Germany (5 min temporally and 1 km spatially) using synoptic cloud observations, to distinguish convective and stratiform rain events. In a second step, for each precipitation type, the observed data are aggregated over a sequence of time intervals and spatial areas. The resulting matrix allows a detailed investigation of the resolutions at which convective or stratiform events are expected to contribute most to the extremes. We analyze where the statistics of the two types differ and discuss at which resolutions transitions occur between dominance of either of the two precipitation types. We characterize the scales at which the convective or stratiform events will dominate the statistics. For both types, we further develop a mapping between pairs of spatially and temporally aggregated statistics. The resulting curve is relevant when deciding on data resolutions where statistical information in space and time is balanced. Our study may hence also serve as a practical guide for modelers, and for planning the space-time layout of measurement campaigns. We also describe a mapping between different pairs of resolutions, possibly relevant when working with mismatched model and observational resolutions, such as in statistical bias correction.

Ultraviolet Observations of M-Type Symbiotic Stars

NASA Astrophysics Data System (ADS)

Michalitsianos, Andrew G.

The significant differences revealed in high dispersion short wavelength spectra of two M-type symbiotic stars RW Hya (gM2 + pec) and RX Pup (M5 + pec) observed previously with IUE emphasizes the need for high resolution observations of a wide range of similar objects. The anomalies observed in high excitation lines in RX Pup of He II, N III], N IV], O III], C III], C IV and Si III] that show split line profiles, multiple component Doppler displaced components, and broadened blue wing emission structure in N III] and N IV] suggest motion in circumstellar material. In contrast, high dispersion UV spectra of RW Hya reveal narrow high excitation emission lines that give no suggestion of macroscopic motions in the circumstellar gas. We wish to extend observations of a selected number of symbiotic stars observed previously but in low resolution, to high dispersion in order to determine if particular M-type symbiotic stars exhibit anomalies in their line profile. As such, symbiotic stars exhibiting velocity structure in emission lines may form a subset of objects that are characterized by mass motions in their circumstellar envelops that create high excitation emission. UV line and continuum emission from other M-type symbiotics may arise from mainly photo-excitation processes that results from the intense radiation field associated with the hot secondary companion.

Computer programs to assist in high resolution thermal denaturation and circular dichroism studies on nucleic acids

PubMed Central

Goodman, Thomas C.; Hardies, Stephen C.; Cortez, Carlos; Hillen, Wolfgang

1981-01-01

Computer programs are described that direct the collection, processing, and graphical display of numerical data obtained from high resolution thermal denaturation (1-3) and circular dichroism (4) studies. Besides these specific applications, the programs may also be useful, either directly or as programming models, in other types of spectrophotometric studies employing computers, programming languages, or instruments similar to those described here (see Materials and Methods). PMID:7335498

Operation Ivy. Project 8. 4. Report to the Scientific Director. High-resolution spectroscopy at Ivy compared with previous tests

DOE Office of Scientific and Technical Information (OSTI.GOV)

Beck, C.A.

1985-04-01

The high-resolution ultraviolet and visible spectra of typical test nuclear detonations up to and including Operation Ivy were analyzed and compared. Topics studied include the types of atomc and molecular material observed (with calculations, in some cases, of the relative quantities involved), the ultraviolet cutoff, and rotational temperatures. Variation of these quantities with the radiochemical yield of the bomb is indicated.

Hydraulic Tomography and High-Resolution Slug Testing to Determine Hydraulic Conductivity Distributions

DTIC Science & Technology

2011-02-01

Research Report Documentation Page Form ApprovedOMB No. 0704-0188 Public reporting burden for the collection of information is estimated to average...REPORT DATE FEB 2011 2. REPORT TYPE N/A 3. DATES COVERED - 4. TITLE AND SUBTITLE Hydraulic Tomography and High-Resolution Slug Testing to...NUMBER 5f. WORK UNIT NUMBER 7. PERFORMING ORGANIZATION NAME(S) AND ADDRESS(ES) University of Kansas Center for Research 8. PERFORMING

Enhanced Deforestation Mapping in North Korea using Spatial-temporal Image Fusion Method and Phenology-based Index

NASA Astrophysics Data System (ADS)

Jin, Y.; Lee, D.

2017-12-01

North Korea (the Democratic People's Republic of Korea, DPRK) is known to have some of the most degraded forest in the world. The characteristics of forest landscape in North Korea is complex and heterogeneous, the major vegetation cover types in the forest are hillside farm, unstocked forest, natural forest, and plateau vegetation. Better classification of types in high spatial resolution of deforested areas could provide essential information for decisions about forest management priorities and restoration of deforested areas. For mapping heterogeneous vegetation covers, the phenology-based indices are helpful to overcome the reflectance value confusion that occurs when using one season images. Coarse spatial resolution images may be acquired with a high repetition rate and it is useful for analyzing phenology characteristics, but may not capture the spatial detail of the land cover mosaic of the region of interest. Previous spatial-temporal fusion methods were only capture the temporal change, or focused on both temporal change and spatial change but with low accuracy in heterogeneous landscapes and small patches. In this study, a new concept for spatial-temporal image fusion method focus on heterogeneous landscape was proposed to produce fine resolution images at both fine spatial and temporal resolution. We classified the three types of pixels between the base image and target image, the first type is only reflectance changed caused by phenology, this type of pixels supply the reflectance, shape and texture information; the second type is both reflectance and spectrum changed in some bands caused by phenology like rice paddy or farmland, this type of pixels only supply shape and texture information; the third type is reflectance and spectrum changed caused by land cover type change, this type of pixels don't provide any information because we can't know how land cover changed in target image; and each type of pixels were applied different prediction methods. Results show that both STARFM and FSDAF predicted in low accuracy in second type pixels and small patches. Classification results used spatial-temporal image fusion method proposed in this study showed overall classification accuracy of 89.38%, with corresponding kappa coefficients of 0.87.

Atomic-resolution transmission electron microscopy of electron beam–sensitive crystalline materials

NASA Astrophysics Data System (ADS)

Zhang, Daliang; Zhu, Yihan; Liu, Lingmei; Ying, Xiangrong; Hsiung, Chia-En; Sougrat, Rachid; Li, Kun; Han, Yu

2018-02-01

High-resolution imaging of electron beam–sensitive materials is one of the most difficult applications of transmission electron microscopy (TEM). The challenges are manifold, including the acquisition of images with extremely low beam doses, the time-constrained search for crystal zone axes, the precise image alignment, and the accurate determination of the defocus value. We develop a suite of methods to fulfill these requirements and acquire atomic-resolution TEM images of several metal organic frameworks that are generally recognized as highly sensitive to electron beams. The high image resolution allows us to identify individual metal atomic columns, various types of surface termination, and benzene rings in the organic linkers. We also apply our methods to other electron beam–sensitive materials, including the organic-inorganic hybrid perovskite CH3NH3PbBr3.

Low resolution spectroscopic investigation of Am stars using Automated method

NASA Astrophysics Data System (ADS)

Sharma, Kaushal; Joshi, Santosh; Singh, Harinder P.

2018-04-01

The automated method of full spectrum fitting gives reliable estimates of stellar atmospheric parameters (Teff, log g and [Fe/H]) for late A, F, G, and early K type stars. Recently, the technique was further improved in the cooler regime and the validity range was extended up to a spectral type of M6 - M7 (Teff˜ 2900 K). The present study aims to explore the application of this method on the low-resolution spectra of Am stars, a class of chemically peculiar stars, to examine its robustness for these objects. We use ULySS with the Medium-resolution INT Library of Empirical Spectra (MILES) V2 spectral interpolator for parameter determination. The determined Teff and log g values are found to be in good agreement with those obtained from high-resolution spectroscopy.

Information Extraction of High Resolution Remote Sensing Images Based on the Calculation of Optimal Segmentation Parameters

PubMed Central

Zhu, Hongchun; Cai, Lijie; Liu, Haiying; Huang, Wei

2016-01-01

Multi-scale image segmentation and the selection of optimal segmentation parameters are the key processes in the object-oriented information extraction of high-resolution remote sensing images. The accuracy of remote sensing special subject information depends on this extraction. On the basis of WorldView-2 high-resolution data, the optimal segmentation parameters methodof object-oriented image segmentation and high-resolution image information extraction, the following processes were conducted in this study. Firstly, the best combination of the bands and weights was determined for the information extraction of high-resolution remote sensing image. An improved weighted mean-variance method was proposed andused to calculatethe optimal segmentation scale. Thereafter, the best shape factor parameter and compact factor parameters were computed with the use of the control variables and the combination of the heterogeneity and homogeneity indexes. Different types of image segmentation parameters were obtained according to the surface features. The high-resolution remote sensing images were multi-scale segmented with the optimal segmentation parameters. Ahierarchical network structure was established by setting the information extraction rules to achieve object-oriented information extraction. This study presents an effective and practical method that can explain expert input judgment by reproducible quantitative measurements. Furthermore the results of this procedure may be incorporated into a classification scheme. PMID:27362762

Information Extraction of High Resolution Remote Sensing Images Based on the Calculation of Optimal Segmentation Parameters.

PubMed

Zhu, Hongchun; Cai, Lijie; Liu, Haiying; Huang, Wei

2016-01-01

Multi-scale image segmentation and the selection of optimal segmentation parameters are the key processes in the object-oriented information extraction of high-resolution remote sensing images. The accuracy of remote sensing special subject information depends on this extraction. On the basis of WorldView-2 high-resolution data, the optimal segmentation parameters methodof object-oriented image segmentation and high-resolution image information extraction, the following processes were conducted in this study. Firstly, the best combination of the bands and weights was determined for the information extraction of high-resolution remote sensing image. An improved weighted mean-variance method was proposed andused to calculatethe optimal segmentation scale. Thereafter, the best shape factor parameter and compact factor parameters were computed with the use of the control variables and the combination of the heterogeneity and homogeneity indexes. Different types of image segmentation parameters were obtained according to the surface features. The high-resolution remote sensing images were multi-scale segmented with the optimal segmentation parameters. Ahierarchical network structure was established by setting the information extraction rules to achieve object-oriented information extraction. This study presents an effective and practical method that can explain expert input judgment by reproducible quantitative measurements. Furthermore the results of this procedure may be incorporated into a classification scheme.

High resolution optical DNA mapping

NASA Astrophysics Data System (ADS)

Baday, Murat

Many types of diseases including cancer and autism are associated with copy-number variations in the genome. Most of these variations could not be identified with existing sequencing and optical DNA mapping methods. We have developed Multi-color Super-resolution technique, with potential for high throughput and low cost, which can allow us to recognize more of these variations. Our technique has made 10--fold improvement in the resolution of optical DNA mapping. Using a 180 kb BAC clone as a model system, we resolved dense patterns from 108 fluorescent labels of two different colors representing two different sequence-motifs. Overall, a detailed DNA map with 100 bp resolution was achieved, which has the potential to reveal detailed information about genetic variance and to facilitate medical diagnosis of genetic disease.

Pulsed-neutron imaging by a high-speed camera and center-of-gravity processing

NASA Astrophysics Data System (ADS)

Mochiki, K.; Uragaki, T.; Koide, J.; Kushima, Y.; Kawarabayashi, J.; Taketani, A.; Otake, Y.; Matsumoto, Y.; Su, Y.; Hiroi, K.; Shinohara, T.; Kai, T.

2018-01-01

Pulsed-neutron imaging is attractive technique in the research fields of energy-resolved neutron radiography and RANS (RIKEN) and RADEN (J-PARC/JAEA) are small and large accelerator-driven pulsed-neutron facilities for its imaging, respectively. To overcome the insuficient spatial resolution of the conunting type imaging detectors like μ NID, nGEM and pixelated detectors, camera detectors combined with a neutron color image intensifier were investigated. At RANS center-of-gravity technique was applied to spots image obtained by a CCD camera and the technique was confirmed to be effective for improving spatial resolution. At RADEN a high-frame-rate CMOS camera was used and super resolution technique was applied and it was recognized that the spatial resolution was futhermore improved.

Potential Long-Term Records of Surface Albedo at Fine Spatiotemporal Resolution from Landsat/Sentinle-2A Surface Reflectance and MODIS/VIIRS BRDF

NASA Astrophysics Data System (ADS)

Li, Z.; Schaaf, C.; Shuai, Y.; Liu, Y.; Sun, Q.; Erb, A.; Wang, Z.

2016-12-01

The land surface albedo products at fine spatial resolutions are generated by coupling surface reflectance (SR) from Landsat (30 m) or Sentinel-2A (20 m) with concurrent surface anisotropy information (the Bidirectional Reflectance Distribution Function - BRDF) at coarser spatial resolutions from sequential multi-angular observations by the Moderate Resolution Imaging Spectroradiometer (MODIS) or its successor, the Visible Infrared Imaging Radiometer Suite (VIIRS). We assess the comparability of four types of fine-resolution albedo products (black-sky and white-sky albedos over the shortwave broad band) generated by coupling, (1) Landsat-8 Optical Land Imager (OLI) SR with MODIS BRDF; (2) OLI SR with VIIRS BRDF; (3) Sentinel-2A MultiSpectral Instrument (MSI) SR with MODIS BRDF; and (4) MSI SR with VIIRS BRDF. We evaluate the accuracy of these four types of fine-resolution albedo products using ground tower measurements of surface albedo over six SURFace RADiation Network (SURFRAD) sites in the United States. For comparison with the ground measurements, we estimate the actual (blue-sky) albedo values at the six sites by using the satellite-based retrievals of black-sky and white-sky albedos and taking into account the proportion of direct and diffuse solar radiation from the ground measurements at the sites. The coupling of the OLI and MSI SR with MODIS BRDF has already been shown to provide accurate fine-resolution albedo values. With demonstration of a high agreement in BRDF products from MODIS and VIIRS, we expect to see consistency between all four types of fine-resolution albedo products. This assurance of consistency between the couplings of both OLI and MSI with both MODIS and VIIRS guarantees the production of long-term records of surface albedo at fine spatial resolutions and an increased temporal resolution. Such products will be critical in studying land surface changes and associated surface energy balance over the dynamic and heterogeneous landscapes most susceptible to climate change (such as arctic, coastal, and high-elevation zones).

Comparison of Dixon Sequences for Estimation of Percent Breast Fibroglandular Tissue

PubMed Central

Ledger, Araminta E. W.; Scurr, Erica D.; Hughes, Julie; Macdonald, Alison; Wallace, Toni; Thomas, Karen; Wilson, Robin; Leach, Martin O.; Schmidt, Maria A.

2016-01-01

Objectives To evaluate sources of error in the Magnetic Resonance Imaging (MRI) measurement of percent fibroglandular tissue (%FGT) using two-point Dixon sequences for fat-water separation. Methods Ten female volunteers (median age: 31 yrs, range: 23–50 yrs) gave informed consent following Research Ethics Committee approval. Each volunteer was scanned twice following repositioning to enable an estimation of measurement repeatability from high-resolution gradient-echo (GRE) proton-density (PD)-weighted Dixon sequences. Differences in measures of %FGT attributable to resolution, T1 weighting and sequence type were assessed by comparison of this Dixon sequence with low-resolution GRE PD-weighted Dixon data, and against gradient-echo (GRE) or spin-echo (SE) based T1-weighted Dixon datasets, respectively. Results %FGT measurement from high-resolution PD-weighted Dixon sequences had a coefficient of repeatability of ±4.3%. There was no significant difference in %FGT between high-resolution and low-resolution PD-weighted data. Values of %FGT from GRE and SE T1-weighted data were strongly correlated with that derived from PD-weighted data (r = 0.995 and 0.96, respectively). However, both sequences exhibited higher mean %FGT by 2.9% (p < 0.0001) and 12.6% (p < 0.0001), respectively, in comparison with PD-weighted data; the increase in %FGT from the SE T1-weighted sequence was significantly larger at lower breast densities. Conclusion Although measurement of %FGT at low resolution is feasible, T1 weighting and sequence type impact on the accuracy of Dixon-based %FGT measurements; Dixon MRI protocols for %FGT measurement should be carefully considered, particularly for longitudinal or multi-centre studies. PMID:27011312

CtGEM typing: Discrimination of Chlamydia trachomatis ocular and urogenital strains and major evolutionary lineages by high resolution melting analysis of two amplified DNA fragments.

PubMed

Giffard, Philip M; Andersson, Patiyan; Wilson, Judith; Buckley, Cameron; Lilliebridge, Rachael; Harris, Tegan M; Kleinecke, Mariana; O'Grady, Kerry-Ann F; Huston, Wilhelmina M; Lambert, Stephen B; Whiley, David M; Holt, Deborah C

2018-01-01

Chlamydia trachomatis infects the urogenital tract (UGT) and eyes. Anatomical tropism is correlated with variation in the major outer membrane protein encoded by ompA. Strains possessing the ocular ompA variants A, B, Ba and C are typically found within the phylogenetically coherent "classical ocular lineage". However, variants B, Ba and C have also been found within three distinct strains in Australia, all associated with ocular disease in children and outside the classical ocular lineage. CtGEM genotyping is a method for detecting and discriminating ocular strains and also the major phylogenetic lineages. The rationale was facilitation of surveillance to inform responses to C. trachomatis detection in UGT specimens from young children. CtGEM typing is based on high resolution melting analysis (HRMA) of two PCR amplified fragments with high combinatorial resolving power, as defined by computerised comparison of 65 whole genomes. One fragment is from the hypothetical gene defined by Jali-1891 in the C. trachomatis B_Jali20 genome, while the other is from ompA. Twenty combinatorial CtGEM types have been shown to exist, and these encompass unique genotypes for all known ocular strains, and also delineate the TI and T2 major phylogenetic lineages, identify LGV strains and provide additional resolution beyond this. CtGEM typing and Sanger sequencing were compared with 42 C. trachomatis positive clinical specimens, and there were no disjunctions. CtGEM typing is a highly efficient method designed and tested using large scale comparative genomics. It divides C. trachomatis into clinically and biologically meaningful groups, and may have broad application in surveillance.

Identification of PTP1B and α-Glucosidase Inhibitory Serrulatanes from Eremophila spp. by Combined use of Dual High-Resolution PTP1B and α-Glucosidase Inhibition Profiling and HPLC-HRMS-SPE-NMR.

PubMed

Wubshet, Sileshi G; Tahtah, Yousof; Heskes, Allison M; Kongstad, Kenneth T; Pateraki, Irini; Hamberger, Björn; Møller, Birger L; Staerk, Dan

2016-04-22

According to the International Diabetes Federation, type 2 diabetes (T2D) has reached epidemic proportions, affecting more than 382 million people worldwide. Inhibition of protein tyrosine phosphatase-1B (PTP1B) and α-glucosidase is a recognized therapeutic approach for management of T2D and its associated complications. The lack of clinical drugs targeting PTP1B and side effects of the existing α-glucosidase drugs, emphasize the need for new drug leads for these T2D targets. In the present work, dual high-resolution PTP1B and α-glucosidase inhibition profiles of Eremophila gibbosa, E. glabra, and E. aff. drummondii "Kalgoorlie" were used for pinpointing α-glucosidase and/or PTP1B inhibitory constituents directly from the crude extracts. A subsequent targeted high-performance liquid chromatography-high-resolution mass spectrometry-solid-phase extraction-nuclear magnetic resonance spectroscopy (HPLC-HRMS-SPE-NMR) analysis and preparative-scale HPLC isolation led to identification of 21 metabolites from the three species, of which 16 were serrulatane-type diterpenoids (12 new) associated with either α-glucosidase and/or PTP1B inhibition. This is the first report of serrulatane-type diterpenoids as potential α-glucosidase and/or PTP1B inhibitors.

Gene Scanning of an Internalin B Gene Fragment Using High-Resolution Melting Curve Analysis as a Tool for Rapid Typing of Listeria monocytogenes

PubMed Central

Pietzka, Ariane T.; Stöger, Anna; Huhulescu, Steliana; Allerberger, Franz; Ruppitsch, Werner

2011-01-01

The ability to accurately track Listeria monocytogenes strains involved in outbreaks is essential for control and prevention of listeriosis. Because current typing techniques are time-consuming, cost-intensive, technically demanding, and difficult to standardize, we developed a rapid and cost-effective method for typing of L. monocytogenes. In all, 172 clinical L. monocytogenes isolates and 20 isolates from culture collections were typed by high-resolution melting (HRM) curve analysis of a specific locus of the internalin B gene (inlB). All obtained HRM curve profiles were verified by sequence analysis. The 192 tested L. monocytogenes isolates yielded 15 specific HRM curve profiles. Sequence analysis revealed that these 15 HRM curve profiles correspond to 18 distinct inlB sequence types. The HRM curve profiles obtained correlated with the five phylogenetic groups I.1, I.2, II.1, II.2, and III. Thus, HRM curve analysis constitutes an inexpensive assay and represents an improvement in typing relative to classical serotyping or multiplex PCR typing protocols. This method provides a rapid and powerful screening tool for simultaneous preliminary typing of up to 384 samples in approximately 2 hours. PMID:21227395

High-resolution α-amylase assay combined with high-performance liquid chromatography-solid-phase extraction-nuclear magnetic resonance spectroscopy for expedited identification of α-amylase inhibitors: proof of concept and α-amylase inhibitor in cinnamon.

PubMed

Okutan, Leyla; Kongstad, Kenneth T; Jäger, Anna K; Staerk, Dan

2014-11-26

Type 2 diabetes affects millions of people worldwide, and new improved drugs or functional foods containing selective α-amylase inhibitors are needed for improved management of blood glucose. In this article the development of a microplate-based high-resolution α-amylase inhibition assay with direct photometric measurement of α-amylase activity is described. The inhibition assay is based on porcine pancreatic α-amylase with 2-chloro-4-nitrophenyl-α-D-maltotriose as substrate, which this gives a stable, sensitive, and cheap inhibition assay as requested for high-resolution purposes. In combination with HPLC-HRMS-SPE-NMR, this provides an analytical platform that allows simultaneous chemical and biological profiling of α-amylase inhibitors in plant extracts. Proof-of-concept with an artificial mixture of six compounds-of which three are known α-amylase inhibitors-showed that the high-resolution α-amylase inhibition profiles allowed detection of sub-microgram amounts of the α-amylase inhibitors. Furthermore, the high-resolution α-amylase inhibition assay/HPLC-HRMS-SPE-NMR platform allowed identification of cinnamaldehyde as the α-amylase inhibitor in cinnamon (Cinnamomum verum Presl.).

Unmanned Aerial Vehicles Produce High-Resolution Seasonally-Relevant Imagery for Classifying Wetland Vegetation

NASA Astrophysics Data System (ADS)

Marcaccio, J. V.; Markle, C. E.; Chow-Fraser, P.

2015-08-01

With recent advances in technology, personal aerial imagery acquired with unmanned aerial vehicles (UAVs) has transformed the way ecologists can map seasonal changes in wetland habitat. Here, we use a multi-rotor (consumer quad-copter, the DJI Phantom 2 Vision+) UAV to acquire a high-resolution (< 8 cm) composite photo of a coastal wetland in summer 2014. Using validation data collected in the field, we determine if a UAV image and SWOOP (Southwestern Ontario Orthoimagery Project) image (collected in spring 2010) differ in their classification of type of dominant vegetation type and percent cover of three plant classes: submerged aquatic vegetation, floating aquatic vegetation, and emergent vegetation. The UAV imagery was more accurate than available SWOOP imagery for mapping percent cover of submergent and floating vegetation categories, but both were able to accurately determine the dominant vegetation type and percent cover of emergent vegetation. Our results underscore the value and potential for affordable UAVs (complete quad-copter system < 3,000 CAD) to revolutionize the way ecologists obtain imagery and conduct field research. In Canada, new UAV regulations make this an easy and affordable way to obtain multiple high-resolution images of small (< 1.0 km2) wetlands, or portions of larger wetlands throughout a year.

Study of the Vibrational Modes of Subsurface Oxygen on Al (111) Using Diode Laser Infrared Reflection-Absorption Spectroscopy.

DTIC Science & Technology

1987-10-15

apparent shift of this band to higher energy with increasing coverage, observed at lower resolution (but higher sensitivity) in electron energy loss...apparent shift of this band to higher energy with increasing coverage, observed at lower resolution (but higher sen- sitivity) in electron energy ...11 using high-resolution electron energy -loss spectroscopy (EELS), is especially intriguing. 02 dissociates on this surface to populate two types of

Multi-resolution voxel phantom modeling: a high-resolution eye model for computational dosimetry

NASA Astrophysics Data System (ADS)

Caracappa, Peter F.; Rhodes, Ashley; Fiedler, Derek

2014-09-01

Voxel models of the human body are commonly used for simulating radiation dose with a Monte Carlo radiation transport code. Due to memory limitations, the voxel resolution of these computational phantoms is typically too large to accurately represent the dimensions of small features such as the eye. Recently reduced recommended dose limits to the lens of the eye, which is a radiosensitive tissue with a significant concern for cataract formation, has lent increased importance to understanding the dose to this tissue. A high-resolution eye model is constructed using physiological data for the dimensions of radiosensitive tissues, and combined with an existing set of whole-body models to form a multi-resolution voxel phantom, which is used with the MCNPX code to calculate radiation dose from various exposure types. This phantom provides an accurate representation of the radiation transport through the structures of the eye. Two alternate methods of including a high-resolution eye model within an existing whole-body model are developed. The accuracy and performance of each method is compared against existing computational phantoms.

Geomorphological diversity of Dong-Sha Atoll based on spectrum and texture analysis in high resolution remote sensing imagery

NASA Astrophysics Data System (ADS)

Chen, Jianyu; Mao, Zhihua; He, Xianqiang

2009-01-01

Coral reefs are complex marine ecosystems that are constructed and maintained by biological communities that thrive in tropical oceans. The Dong-Sha Atoll is located at the northern continental margin of the South China Sea. It has being abused by destructive activity of human being and natural event during recent decades. Remote sensing offers a powerful tool for studying coral reef geomorphology and is the most cost-effective approach for large-scale reef survey. In this paper, the high-resolution Quickbird2 imageries which covered the full atoll are used to categorize the current distribution of coral reefs geomorphological structure therein with the auxiliary SPOT5 and ASTER imageries. Spectral and texture analysis are used to distinguish the geomorphological diversity during data processing. The Gray Level Co-occurrence Matrices is adopted for texture feature extraction and atoll geomorphology mapping in the high-resolution pan-color image of Quickbird2. Quickbird2 is considered as the most appropriate image source for coral reefs studies. In the Dong-Sha Atoll, various dynamical geomorphologic units are developed according to wave energy zones. There the reef frame types are classified to 3 different types according as its diversity at the image. The radial structure system is the most characteristic and from high resolution imagery we can distinguish the discrepancy between them.

Development of a high resolution plantar pressure monitoring pad based on fiber Bragg grating (FBG) sensors.

PubMed

Suresh, R; Bhalla, S; Hao, J; Singh, C

2015-01-01

High importance is given to plantar pressure monitoring in the field of biomedical engineering for the diagnosis of posture related ailments associated with diseases such as diabetes and gonarthrosis. This paper presents the proof-of-concept development of a new high resolution plantar pressure monitoring pad based on fiber Bragg grating (FBG) sensors. In the proposed configuration, the FBG sensors are embedded within layers of carbon composite material (CCM) in turn conforming to an arc shape. A total of four such arc shaped sensors are instrumented in the pad at the locations of the forefoot and the hind foot. As a test of the pad, static plantar pressure is monitored on normal subjects under various posture conditions. The pad is evaluated both as a standalone platform as well as a pad inserted inside a standard shoe. An average pressure sensitivity of 1.2 pm/kPa and a resolution of approximately 0.8 kPa is obtained in this special configuration. The pad is found to be suitable in both configurations- stand-alone pad as well as an insert inside a standard shoe. The proposed set up offers a cost-effective high resolution and accurate plantar pressure measurement system suitable for clinical deployment. The novelty of the developed pressure pad lies in its ability to be used both as platform type as well as inserted in-sole type sensor system.

Resolution of inflammation by interleukin-9-producing type 2 innate lymphoid cells

PubMed Central

Rauber, Simon; Luber, Markus; Weber, Stefanie; Maul, Lisa; Soare, Alina; Wohlfahrt, Thomas; Lin, Neng-Yu; Dietel, Katharina; Bozec, Aline; Herrmann, Martin; Kaplan, Mark H.; Weigmann, Benno; Zaiss, Mario M.; Fearon, Ursula; Veale, Douglas J.; Canete, Juan D.; Distler, Oliver; Rivellese, Felice; Pitzalis, Costantino; Neurath, Markus F.; McKenzie, Andrew N.J.; Wirtz, Stefan; Schett, Georg; Distler, Jörg H.W.; Ramming, Andreas

2017-01-01

Inflammatory diseases such as arthritis are chronic conditions that fail to resolve spontaneously. While the cytokine and cellular pathways triggering arthritis are well defined, those responsible for the resolution of inflammation are incompletely characterized. Here we identified IL-9-producing type 2 innate lymphoid cells (ILC2s) as a molecular and cellular pathway that orchestrates the resolution of chronic inflammation. In mice, the absence of IL-9 impaired ILC2 proliferation, activation of regulatory T cells (Treg) and resulted in chronic arthritis with excessive cartilage destruction and bone loss. In contrast, treatment with IL-9 promoted ILC2-dependent Treg activation and effectively induced resolution of inflammation and protection of bone. Rheumatoid arthritis patients in remission demonstrated high numbers of IL-9+ ILC2s in the joints and in the circulation. Hence, fostering IL-9-mediated ILC2 activation may offer a novel therapeutic approach inducing resolution of inflammation rather than suppression of inflammatory responses. PMID:28714991

High-Resolution Imaging by Adaptive Optics Scanning Laser Ophthalmoscopy Reveals Two Morphologically Distinct Types of Retinal Hard Exudates

PubMed Central

Yamaguchi, Muneo; Nakao, Shintaro; Kaizu, Yoshihiro; Kobayashi, Yoshiyuki; Nakama, Takahito; Arima, Mitsuru; Yoshida, Shigeo; Oshima, Yuji; Takeda, Atsunobu; Ikeda, Yasuhiro; Mukai, Shizuo; Ishibashi, Tatsuro; Sonoda, Koh-hei

2016-01-01

Histological studies from autopsy specimens have characterized hard exudates as a composition of lipid-laden macrophages or noncellular materials including lipid and proteinaceous substances (hyaline substances). However, the characteristics of hard exudates in living patients have not been examined due to insufficient resolution of existing equipment. In this study, we used adaptive optics scanning laser ophthalmoscopy (AO-SLO) to examine the characteristics of hard exudates in patients with retinal vascular diseases. High resolution imaging using AO-SLO enables morphological classification of retinal hard exudates into two types, which could not be distinguished either on fundus examination or by spectral domain optical coherence tomography (SD-OCT). One, termed a round type, consisted of an accumulation of spherical particles (average diameter of particles: 26.9 ± 4.4 μm). The other, termed an irregular type, comprised an irregularly shaped hyper-reflective deposition. The retinal thickness in regions with round hard exudates was significantly greater than the thickness in regions with irregular hard exudates (P = 0.01 →0.02). This differentiation of retinal hard exudates in patients by AO-SLO may help in understanding the pathogenesis and clinical prognosis of retinal vascular diseases. PMID:27641223

High resolution FTIR spectroscopic study of the ν4 band of CH 3CHF 2 enclosed in a flow of cold N 2 gas

NASA Astrophysics Data System (ADS)

Appadoo, Dominique R. T.; Robertson, Evan G.; McNaughton, Don

2003-01-01

An enclosive flow cooling (EFC) cell has been constructed, and coupled to a Brüker IFS 120HR high resolution Fourier transform spectrometer to record rotationally cold absorption spectra of gases of atmospheric interest at high spectral resolution. The new system has been characterized using N 2O, revealing that rotational temperatures as cold as 110 K are readily attainable using liquid nitrogen as a cryogen. Infrared spectra of the ν4 band of 1,1-difluoroethane (R152a), CH 3CHF 2, cooled in the EFC cell have been measured at a resolution of 0.0019 cm-1. Eight hundred and twenty rovibrational transitions of the weak ν4 band with 2⩽ J'⩽46 and Kc'⩽16 were assigned and fitted to Watson's A-reduced Hamiltonian. The ν4 CH 3 symmetric deformation ( a/c-type) was found to be coupled to the ν13 asymmetric deformation ( b-type) via an a-axis Coriolis interaction. In the ensuing analysis, values of spectroscopic constants were obtained for both the ν4 and dark ν13 states. Supporting ab initio calculations up to the MP2/TZV+(3 df,3 p) level are presented.

Interface control document between the NASA Goddard Space Flight Center (GSFC) and Department of Interior EROS Data Center (EDC) for LANDSAT-D. Thematic mapper high resolution 241 mm film

NASA Technical Reports Server (NTRS)

1982-01-01

The 241 mm photographic product produced by the Goddard Space Flight Center Data Management System for LANDSAT-D is described. Film type and format, image dimensions, frame ID, gray scale, resolution patterns, registration marks, etc. are addressed.

Spectroscopic monitoring of bright A-F type candidate hybrid stars discovered by the Kepler mission

NASA Astrophysics Data System (ADS)

Lampens, Patricia; Frémat, Y.; Vermeylen, Lore; De Cat, Peter; Dumortier, Louis; Sódor, Ádám; Sharka, Marek; Bognár, Zsófia

2018-04-01

We report on a study of 250 optical spectra for 50 bright A/F-type candidate hybrid pulsating stars from the Kepler field. Most of the spectra have been collected with the high-resolution spectrograph HERMES attached to the Mercator telescope, La Palma. We determined the radial velocities (RVs), projected rotational velocities, fundamental atmospheric parameters and provide a classification based on the appearance of the cross-correlation profiles and the behaviour of the RVs with time in order to find true hybrid pulsators. Additionally, we also detected new spectroscopic binary and multiple systems in our sample and determined the fraction of spectroscopic systems. In order to be able to extend this investigation to the fainter A-F type candidate hybrid stars, various high-quality spectra collected with 3-4 m sized telescopes suitably equipped with a high-resolution spectrograph and furthermore located in the Northern hemisphere would be ideal. This programme could be done using the new instruments installed at the Devasthal Observatory.

The scale dependence of optical diversity in a prairie ecosystem

NASA Astrophysics Data System (ADS)

Gamon, J. A.; Wang, R.; Stilwell, A.; Zygielbaum, A. I.; Cavender-Bares, J.; Townsend, P. A.

2015-12-01

Biodiversity loss, one of the most crucial challenges of our time, endangers ecosystem services that maintain human wellbeing. Traditional methods of measuring biodiversity require extensive and costly field sampling by biologists with extensive experience in species identification. Remote sensing can be used for such assessment based upon patterns of optical variation. This provides efficient and cost-effective means to determine ecosystem diversity at different scales and over large areas. Sampling scale has been described as a "fundamental conceptual problem" in ecology, and is an important practical consideration in both remote sensing and traditional biodiversity studies. On the one hand, with decreasing spatial and spectral resolution, the differences among different optical types may become weak or even disappear. Alternately, high spatial and/or spectral resolution may introduce redundant or contradictory information. For example, at high resolution, the variation within optical types (e.g., between leaves on a single plant canopy) may add complexity unrelated to specie richness. We studied the scale-dependence of optical diversity in a prairie ecosystem at Cedar Creek Ecosystem Science Reserve, Minnesota, USA using a variety of spectrometers from several platforms on the ground and in the air. Using the coefficient of variation (CV) of spectra as an indicator of optical diversity, we found that high richness plots generally have a higher coefficient of variation. High resolution imaging spectrometer data (1 mm pixels) showed the highest sensitivity to richness level. With decreasing spatial resolution, the difference in CV between richness levels decreased, but remained significant. These findings can be used to guide airborne studies of biodiversity and develop more effective large-scale biodiversity sampling methods.

Micromachined PIN-PMN-PT crystal composite transducer for high-frequency intravascular ultrasound (IVUS) imaging.

PubMed

Li, Xiang; Ma, Teng; Tian, Jian; Han, Pengdi; Zhou, Qifa; Shung, K Kirk

2014-07-01

In this paper, we report the use of micromachined PbIn1/2Nb1/2O3-PbMg1/3Nb2/3O3-PbTiO 3 (PIN-PMNPT) single crystal 1-3 composite material for intravascular ultrasound (IVUS) imaging application. The effective electromechanical coupling coefficient kt(eff) of the composite was measured to be 0.75 to 0.78. Acoustic impedance was estimated to be 20 MRayl. Based on the composite, needle-type and flexible-type IVUS transducers were fabricated. The composite transducer achieved an 86% bandwidth at the center frequency of 41 MHz, which resulted in a 43 μm axial resolution. Ex vivo IVUS imaging was conducted to demonstrate the improvement of axial resolution. The composite transducer was capable of identifying the three layers of a cadaver coronary artery specimen with high resolution. The PIN-PMN-PT-based composite has superior piezoelectric properties comparable to PMN-PT-based composite and its thermal stability is higher than PMN-PT. PIN-PMN-PT crystal can be an alternative approach for fabricating high-frequency composite, instead of using PMN-PT.

Libraries of High and Mid-Resolution Spectra of F, G, K, and M Field Stars

NASA Astrophysics Data System (ADS)

Montes, D.

1998-06-01

I have compiled here the three libraries of high and mid-resolution optical spectra of late-type stars I have recently published. The libraries include F, G, K and M field stars, from dwarfs to giants. The spectral coverage is from 3800 to 1000 Å, with spectral resolution ranging from 0.09 to 3.0 Å. These spectra include many of the spectral lines most widely used as optical and near-infrared indicators of chromospheric activity. The spectra have been obtained with the aim of providing a library of high and mid-resolution spectra to be used in the study of active chromosphere stars by applying a spectral subtraction technique. However, the data set presented here can also be utilized in a wide variety of ways. A digital version of all the fully reduced spectra is available via FTP and the World Wide Web (WWW) in FITS format.

Estimating chlorophyll with thermal and broadband multispectral high resolution imagery from an unmanned aerial system using relevance vector machines for precision agriculture

NASA Astrophysics Data System (ADS)

Elarab, Manal; Ticlavilca, Andres M.; Torres-Rua, Alfonso F.; Maslova, Inga; McKee, Mac

2015-12-01

Precision agriculture requires high-resolution information to enable greater precision in the management of inputs to production. Actionable information about crop and field status must be acquired at high spatial resolution and at a temporal frequency appropriate for timely responses. In this study, high spatial resolution imagery was obtained through the use of a small, unmanned aerial system called AggieAirTM. Simultaneously with the AggieAir flights, intensive ground sampling for plant chlorophyll was conducted at precisely determined locations. This study reports the application of a relevance vector machine coupled with cross validation and backward elimination to a dataset composed of reflectance from high-resolution multi-spectral imagery (VIS-NIR), thermal infrared imagery, and vegetative indices, in conjunction with in situ SPAD measurements from which chlorophyll concentrations were derived, to estimate chlorophyll concentration from remotely sensed data at 15-cm resolution. The results indicate that a relevance vector machine with a thin plate spline kernel type and kernel width of 5.4, having LAI, NDVI, thermal and red bands as the selected set of inputs, can be used to spatially estimate chlorophyll concentration with a root-mean-squared-error of 5.31 μg cm-2, efficiency of 0.76, and 9 relevance vectors.

Using Small Unmanned Aerial Systems to Advance Hydrological Models in Coastal Watersheds

NASA Astrophysics Data System (ADS)

Moorhead, R.; Hathcock, L.; Coffey, J. J.; Hood, R. E.; van Cooten, S.; Choate, K.; Rawson, H.; Kosturock, A.

2014-12-01

Small unmanned aerial systems (sUASs) have the potential to provide highly useful information for models of earth systems that vary over time intervals of days and for which sub-meter resolution is crucial. In particular, the state of coastal watershed plains are highly dependent on vegetation type and cover, soil type, weather, river flooding, and coastal inundation. The vegetation type and cover affect the drying potential, as well as the watershed's resistance to flood water movement. The soil type, soil moisture, and pond depths affect the ability of the watershed to absorb river flood waters and inundation from the sea. In this presentation we will describe a data collection campaign and model modification effort for hydrological models in a coastal watershed. The data collection campaign is obtaining data bimonthly using multiple UASs to capture the state of the watershed quicker. In particular, the vegetation cover and the extent of the water surface expression are captured at approximately a 1 inch spatial resolution over a few days with sUASs that can image 1-2 square miles per hour. The vegetation data provides a time-varying input to improve the estimation of the roughness coefficient and the dry potential from the traditionally static datasets. By correlating the high spatio-temporal resolution surface water expression with data from approximately ten river gauges, models can be improved and validated under more conditions. The presentation will also discuss the requisite sUAS capabilities and our experience in using them.

On the Role of Urban and Vegetative Land Cover in the Identification of Tornado Damage Using Dual-Resolution Multispectral Satellite Imagery

NASA Astrophysics Data System (ADS)

Kingfield, D.; de Beurs, K.

2014-12-01

It has been demonstrated through various case studies that multispectral satellite imagery can be utilized in the identification of damage caused by a tornado through the change detection process. This process involves the difference in returned surface reflectance between two images and is often summarized through a variety of ratio-based vegetation indices (VIs). Land cover type plays a large contributing role in the change detection process as the reflectance properties of vegetation can vary based on several factors (e.g. species, greenness, density). Consequently, this provides the possibility for a variable magnitude of loss, making certain land cover regimes less reliable in the damage identification process. Furthermore, the tradeoff between sensor resolution and orbital return period may also play a role in the ability to detect catastrophic loss. Moderate resolution imagery (e.g. Moderate Resolution Imaging Spectroradiometer (MODIS)) provides relatively coarse surface detail with a higher update rate which could hinder the identification of small regions that underwent a dynamic change. Alternatively, imagery with higher spatial resolution (e.g. Landsat) have a longer temporal return period between successive images which could result in natural recovery underestimating the absolute magnitude of damage incurred. This study evaluates the role of land cover type and sensor resolution on four high-end (EF3+) tornado events occurring in four different land cover groups (agriculture, forest, grassland, urban) in the spring season. The closest successive clear images from both Landsat 5 and MODIS are quality controlled for each case. Transacts of surface reflectance across a homogenous land cover type both inside and outside the damage swath are extracted. These metrics are synthesized through the calculation of six different VIs to rank the calculated change metrics by land cover type, sensor resolution and VI.

A strontium and neodymium isotopic study of Apollo 17 high-Ti mare basalts: Resolution of ages, evolution of magmas, and origins of source heterogeneities

DOE Office of Scientific and Technical Information (OSTI.GOV)

Paces, J.B.; Neal, C.R.; Taylor, L.A.

1991-07-01

A combined Sr and Nd isotopic study of 15 Apollo 17 high-Ti mare basalts was undertaken to investigate geochronological and compositional differences between previously identified magma types (A, B1, B2, and C). Whole-rock and mineral separates for one of the least-evolved Type B1 basalts, 70139, yield Sm-Nd and Rb-Sr isochron ages of 3.71 {plus minus} 0.12 Ga and 3.65 {plus minus} 0.07 Ga and a Rb-Sr isochron age of 3.67 {plus minus} 0.10 Ga. Although these two ages are non-resolvable by themselves, compilation of all available geochronological data allows resolution of Type A and B1/B2 ages at high levels ofmore » confidence (> 99%). The most reliably dated samples, classified according to their geochemical type, yield weighted average ages of 3.75 {plus minus} 0.02 Ga for Type A (N = 4) and 3.69 {plus minus} 0.02 Ga for Type B1/B2 (N = 3) basalts. Insufficient geochronological data are available to place the rare, Type C basalts within this stratigraphy. The authors propose that age differences correlate with geochemical magma type, and that early magmatism was dominated by eruption of Type A basalts while later activity was dominated by effusion of Type B1 and B2 basalts.« less

Final Report: High Spectral Resolution Atmospheric Emitted Radiance Studies with the ARM UAV

DOE Office of Scientific and Technical Information (OSTI.GOV)

Revercomb, Henry E.

1999-12-31

The active participation in the Atmospheric Radiation Measurement (ARM) Unmanned Airborne Vehicle (UAV) science team that was anticipated in the grant proposal was indefinitely delayed after the first year due to a programmatic decision to exclude the high spectral resolution observations from the existing ARM UAV program. However, this report shows that substantial progress toward the science objectives of this grant have made with the help of separate funding from NASA and other agencies. In the four year grant period (including time extensions), a new high spectral resolution instrument has been flown and has successfully demonstrated the ability to obtainmore » measurements of the type needed in the conduct of this grant. In the near term, the third water vapor intensive observing period (WVIOP-3) in October 2000 will provide an opportunity to bring the high spectral resolution observations of upwelling radiance into the ARM program to complement the downwelling radiance observations from the existing ARM AERI instruments. We look forward to a time when the ARM-UAV program is able to extend its scope to include the capability for making these high spectral resolution measurements from a UAV platform.« less

High-resolution high-efficiency multilayer Fresnel zone plates for soft and hard x-rays

NASA Astrophysics Data System (ADS)

Sanli, Umut T.; Keskinbora, Kahraman; Gregorczyk, Keith; Leister, Jonas; Teeny, Nicolas; Grévent, Corinne; Knez, Mato; Schütz, Gisela

2015-09-01

X-ray microscopy enables high spatial resolutions, high penetration depths and characterization of a broad range of materials. Calculations show that nanometer range resolution is achievable in the hard X-ray regime by using Fresnel zone plates (FZPs) if certain conditions are satisfied. However, this requires, among other things, aspect ratios of several thousands. The multilayer (ML) type FZPs, having virtually unlimited aspect ratios, are strong candidates to achieve single nanometer resolutions. Our research is focused on the fabrication of ML-FZPs which encompasses deposition of multilayers over a glass fiber via the atomic layer deposition (ALD), which is subsequently sliced in the optimum thickness for the X-ray energy by a focused ion beam (FIB). We recently achieved aberration free imaging by resolving 21 nm features with an efficiency of up to 12.5 %, the highest imaging resolution achieved by an ML-FZP. We also showed efficient focusing of 7.9 keV X-rays down to 30 nm focal spot size (FWHM). For resolutions below ~10 nm, efficiencies would decrease significantly due to wave coupling effects. To compensate this effect high efficiency, low stress materials have to be researched, as lower intrinsic stresses will allow fabrication of larger FZPs with higher number of zones, leading to high light intensity at the focus. As a first step we fabricated an ML-FZP with a diameter of 62 μm, an outermost zone width of 12 nm and 452 active zones. Further strategies for fabrication of high resolution high efficiency multilayer FZPs will also be discussed.

THE CRAB PULSAR AT CENTIMETER WAVELENGTHS. II. SINGLE PULSES

DOE Office of Scientific and Technical Information (OSTI.GOV)

Hankins, T. H.; Eilek, J. A.; Jones, G., E-mail: thankins@aoc.nrao.edu

2016-12-10

We have carried out new, high-frequency, high-time-resolution observations of the Crab pulsar. Combining these with our previous data, we characterize bright single pulses associated with the Main Pulse, both the Low-Frequency and High-Frequency Interpulses, and the two  High-Frequency Components. Our data include observations at frequencies ranging from 1 to 43 GHz with time resolutions down to a fraction of a nanosecond. We find that at least two types of emission physics are operating in this pulsar. Both Main Pulses and Low-Frequency Interpulses, up to ∼10 GHz, are characterized by nanoshot emission—overlapping clumps of narrowband nanoshots, each with its own polarization signature.more » High-Frequency Interpulses, between 5 and 30 GHz, are characterized by spectral band emission—linearly polarized emission containing ∼30 proportionately spaced spectral bands. We cannot say whether the longer-duration High-Frequency Components pulses are due to a scattering process, or if they come from yet another type of emission physics.« less

Neutron-capture element abundances in the planetary nebula NGC 5315 from deep high-resolution optical and near-IR spectrophotometry

NASA Astrophysics Data System (ADS)

Madonna, S.; García-Rojas, J.; Sterling, N. C.; Luridiana, V.

2017-03-01

We have done a spectroscopical analysis of the type I planetary nebula (PN) NGC 5315, through high-resolution (R ˜ 40000) optical spectroscopy with UVES at the 8.2m Very Large Telescope, and medium-resolution (R ˜ 4800) near-IR spectroscopy with FIRE at the 6.5m Magellan Baade telescope, covering a wide spectral range from 0.31 μm to 2.50μm. The main aim of this work is to investigate the slow neutron(n)-capture process (the s-process) in the Asymptotic Giant Branch (AGB) star progenitor of a type I PNe. We detected and identified about 700 features, including lines from the n-capture elements Kr, Se, and possibly Br and Xe. We compute physical conditions using line ratios of common ions. Ionic abundances are computed for the species with available atomic data. We calculate total abundances using recent ionization correction factors (ICFs) or by summing ionic abundances. Our results for common elements are in good agreement with previous works on the same object. We do not find a substantial s-process enrichment in NGC 5315, which is typical for type I PNe.

Solar flare microwave observations with high spectral resolution

NASA Astrophysics Data System (ADS)

Bruggmann, G.; Magun, A.; Benz, A. O.; Stehling, W.

1990-12-01

The solar flare radio emission in the 6-8 GHz range was observed with a high resolution spectrometer. The observed band corresponds to the plasma frequencies and gyrofrequencies of the transition region and the lowest part of the corona in active regions. Most of the emissions were found to be broadbanded, as expected from the gyrosynchrotron mechanism. In eight out of 46 observed events, spectral structures of three types were detected: spikes below the time resolution of 100 ms, slowly drifting broadband structures, and a narrow bandwidth patch of continuum emission. These first narrowband bursts spectrally recorded in the 6-8 GHz range are generally weak. Slowly drifting structures are the only type compatible with the gyrosynchrotron emission mechanism. A simple argument based on free-free absorption shows that plasma emission can only be propagated if the radiation originates in a dense region with a small density-scale length. The same holds for maser emission at a low harmonic of the electron gyrofrequency. Possible emission mechanisms and diagnostic capabilities are discussed.

Large gamma-ray detector arrays and electromagnetic separators

NASA Astrophysics Data System (ADS)

Lee, I.-Yang

2013-12-01

The use of large gamma-ray detector arrays with electromagnetic separators is a powerful combination. Various types of gamma-ray detectors have been used; some provide high detector efficiency such as scintillation detector array, others use Ge detectors for good energy resolution, and recently developed Ge energy tracking arrays gives both high peak-to-background ratio and position resolution. Similarly, different types of separators were used to optimize the performance under different experimental requirements and conditions. For example, gas-filled separators were used in heavy element studies for their large efficiency and beam rejection factor. Vacuum separators with good isotope resolution were used in transfer and fragmentation reactions for the study of nuclei far from stability. This paper presents results from recent experiments using gamma-ray detector arrays in combination with electromagnetic separators, and discusses the physics opportunities provided by these instruments. In particular, we review the performance of the instruments currently in use, and discuss the requirements of instruments for future radioactive beam accelerator facilities.

NAOMI instrument: a product line of compact and versatile cameras designed for high resolution missions in Earth observation

NASA Astrophysics Data System (ADS)

Luquet, Ph.; Chikouche, A.; Benbouzid, A. B.; Arnoux, J. J.; Chinal, E.; Massol, C.; Rouchit, P.; De Zotti, S.

2017-11-01

EADS Astrium is currently developing a new product line of compact and versatile instruments for high resolution missions in Earth Observation. First version has been developed in the frame of the ALSAT-2 contract awarded by the Algerian Space Agency (ASAL) to EADS Astrium. The Silicon Carbide Korsch-type telescope coupled with a multilines detector array offers a 2.5 m GSD in PAN band at Nadir @ 680 km altitude (10 m GSD in the four multispectral bands) with a 17.5 km swath width. This compact camera - 340 (W) x 460 (L) x 510 (H) mm3, 13 kg - is embarked on a Myriade-type small platform. The electronics unit accommodates video, housekeeping, and thermal control functions and also a 64 Gbit mass memory. Two satellites are developed; the first one is planned to be launched on mid 2009. Several other versions of the instrument have already been defined with enhanced resolution or/and larger field of view.

230Th-U dating of surficial deposits using the ion microprobe (SHRIMP-RG): A microstratigraphic perspective

USGS Publications Warehouse

Maher, K.; Wooden, J.L.; Paces, J.B.; Miller, D.M.

2007-01-01

We used the sensitive high-resolution ion microprobe reverse-geometry (SHRIMP-RG) to date pedogenic opal using the 230Th-U system. Due to the high-spatial resolution of an ion microprobe (typically 30 ??m), regions of pure opal within a sample can be targeted and detrital material can be avoided. In addition, because the technique is non-destructive, the sample can be preserved for other types of analyses including electron microprobe or other stable isotope or trace element ion microprobe measurements. The technique is limited to material with U concentrations greater than ???50 ppm. However, the high spatial resolution, small sample requirements, and the ability to avoid detrital material make this technique a suitable technique for dating many Pleistocene deposits formed in semi-arid environments. To determine the versatility of the method, samples from several different deposits were analyzed, including silica-rich pebble coatings from pedogenic carbonate horizons, a siliceous sinter deposit, and opaline silica deposited as a spring mound. U concentrations for 30-??m-diameter spots ranged from 50 to 1000 ppm in these types of materials. The 230Th/232Th activity ratios also ranged from ???100 to 106, eliminating the need for detrital Th corrections that reduce the precision of traditional U-Th ages for many milligram- and larger-sized samples. In pedogenic material, layers of high-U opal (ca. 500 ppm) are commonly juxtaposed next to layers of calcite with much lower U concentrations (1-2 ppm). If these types of samples are not analyzed using a technique with the appropriate spatial resolution, the ages may be strongly biased towards the age of the opal. Comparison with standard TIMS (Thermal Ionization Mass Spectrometry) measurements from separate microdrilled samples suggests that although the analytical precision of the ion microprobe (SHRIMP-RG) measurements is less than TIMS, the high spatial resolution results in better accuracy in the age determination for finely layered or complex deposits. The ion microprobe approach also may be useful for pre-screening samples to determine the age and degree of post-depositional alteration, analyzing finely layered samples or samples with complex growth histories, and obtaining simultaneous measurements of trace elements.

Spatial-Resolution Cell Type Proteome Profiling of Cancer Tissue by Fully Integrated Proteomics Technology.

PubMed

Xu, Ruilian; Tang, Jun; Deng, Quantong; He, Wan; Sun, Xiujie; Xia, Ligang; Cheng, Zhiqiang; He, Lisheng; You, Shuyuan; Hu, Jintao; Fu, Yuxiang; Zhu, Jian; Chen, Yixin; Gao, Weina; He, An; Guo, Zhengyu; Lin, Lin; Li, Hua; Hu, Chaofeng; Tian, Ruijun

2018-05-01

Increasing attention has been focused on cell type proteome profiling for understanding the heterogeneous multicellular microenvironment in tissue samples. However, current cell type proteome profiling methods need large amounts of starting materials which preclude their application to clinical tumor specimens with limited access. Here, by seamlessly combining laser capture microdissection and integrated proteomics sample preparation technology SISPROT, specific cell types in tumor samples could be precisely dissected with single cell resolution and processed for high-sensitivity proteome profiling. Sample loss and contamination due to the multiple transfer steps are significantly reduced by the full integration and noncontact design. H&E staining dyes which are necessary for cell type investigation could be selectively removed by the unique two-stage design of the spintip device. This easy-to-use proteome profiling technology achieved high sensitivity with the identification of more than 500 proteins from only 0.1 mm 2 and 10 μm thickness colon cancer tissue section. The first cell type proteome profiling of four cell types from one colon tumor and surrounding normal tissue, including cancer cells, enterocytes, lymphocytes, and smooth muscle cells, was obtained. 5271, 4691, 4876, and 2140 protein groups were identified, respectively, from tissue section of only 5 mm 2 and 10 μm thickness. Furthermore, spatially resolved proteome distribution profiles of enterocytes, lymphocytes, and smooth muscle cells on the same tissue slices and across four consecutive sections with micrometer distance were successfully achieved. This fully integrated proteomics technology, termed LCM-SISPROT, is therefore promising for spatial-resolution cell type proteome profiling of tumor microenvironment with a minute amount of clinical starting materials.

High-Resolution Melting Analysis for Rapid Detection of Sequence Type 131 Escherichia coli.

PubMed

Harrison, Lucas B; Hanson, Nancy D

2017-06-01

Escherichia coli isolates belonging to the sequence type 131 (ST131) clonal complex have been associated with the global distribution of fluoroquinolone and β-lactam resistance. Whole-genome sequencing and multilocus sequence typing identify sequence type but are expensive when evaluating large numbers of samples. This study was designed to develop a cost-effective screening tool using high-resolution melting (HRM) analysis to differentiate ST131 from non-ST131 E. coli in large sample populations in the absence of sequence analysis. The method was optimized using DNA from 12 E. coli isolates. Singleplex PCR was performed using 10 ng of DNA, Type-it HRM buffer, and multilocus sequence typing primers and was followed by multiplex PCR. The amplicon sizes ranged from 630 to 737 bp. Melt temperature peaks were determined by performing HRM analysis at 0.1°C resolution from 50 to 95°C on a Rotor-Gene Q 5-plex HRM system. Derivative melt curves were compared between sequence types and analyzed by principal component analysis. A blinded study of 191 E. coli isolates of ST131 and unknown sequence types validated this methodology. This methodology returned 99.2% specificity (124 true negatives and 1 false positive) and 100% sensitivity (66 true positives and 0 false negatives). This HRM methodology distinguishes ST131 from non-ST131 E. coli without sequence analysis. The analysis can be accomplished in about 3 h in any laboratory with an HRM-capable instrument and principal component analysis software. Therefore, this assay is a fast and cost-effective alternative to sequencing-based ST131 identification. Copyright © 2017 Harrison and Hanson.

First results from the Goddard High-Resolution spectrograph - High-resolution observations of the 1942 A resonance line of HG II in the chemically peculiar B star, Chi Lupi

NASA Technical Reports Server (NTRS)

Leckrone, David S.; Wahlgren, Glenn M.; Johansson, Sveneric G.

1991-01-01

The Goddard High-Resolution Spectrograph on the HST has been used to obtain high S/N observations of the sharp-lined, Hg- and Pt-rich B-type star, Chi Lupi, with a resolving power of 87,000. The observations reveal a level of spectroscopic detail never before observed at ultraviolet wavelengths for any star other than the sun. Concentrating on the region around the resonance line of Hg II at 1942 A, the profile and central position of this line confirm beyond doubt that the Hg isotope anomaly in Chi Lupi is real and extreme, with Hg being heavily concentrated in the form of Hg-204. The problems in atomic physics which impair the accurate analysis of spectra of this quality are emphasized.

Development of an S-band cavity-type beam position monitor for a high power THz free-electron laser

NASA Astrophysics Data System (ADS)

Noh, Seon Yeong; Kim, Eun-San; Hwang, Ji-Gwang; Heo, A.; won Jang, Si; Vinokurov, Nikolay A.; Jeong, Young UK; Hee Park, Seong; Jang, Kyu-Ha

2015-01-01

A cavity-type beam position monitor (BPM) has been developed for a compact terahertz (THz) free-electron laser (FEL) system and ultra-short pulsed electron Linac system at the Korea Atomic Energy Research Institute (KAERI). Compared with other types of BPMs, the cavity-type BPM has higher sensitivity and faster response time even at low charge levels. When electron beam passes through the cavity-type BPM, it excites the dipole mode of the cavity of which amplitude depends linearly on the beam offset from the center of the cavity. Signals from the BPM were measured as a function of the beam offset by using an oscilloscope. The microtron accelerator for the KAERI THz FEL produces the electron beam with an energy of 6.5 MeV and pulse length of 5 μs with a micropulse of 10-20 ps at the frequency of 2.801 GHz. The macropulse beam current is 40 mA. Because the microtron provides multi-bunch system, output signal would be the superposition of each single bunch. So high output signal can be obtained from superposition of each single bunch. The designed position resolution of the cavity-type BPM in multi-bunch is submicron. Our cavity-type BPM is made of aluminum and vacuum can be maintained by indium sealing without brazing process, resulting in easy modification and cost saving. The resonance frequency of the cavity-type BPM is 2.803 GHz and the cavity-type BPM dimensions are 200 × 220 mm (length × height) with a pipe diameter of 38 mm. The measured position sensitivity was 6.19 (mV/mm)/mA and the measured isolation between the X and Y axis was -39 dB. By measuring the thermal noise of system, position resolution of the cavity-type BPM was estimated to be less than 1 μm. In this article, we present the test results of the S-band cavity-type BPM and prove the feasibility of the beam position measurement with high resolution using this device.

Development of an S-band cavity-type beam position monitor for a high power THz free-electron laser.

PubMed

Noh, Seon Yeong; Kim, Eun-San; Hwang, Ji-Gwang; Heo, A; Jang, Si won; Vinokurov, Nikolay A; Jeong, Young U K; Park, Seong Hee; Jang, Kyu-Ha

2015-01-01

A cavity-type beam position monitor (BPM) has been developed for a compact terahertz (THz) free-electron laser (FEL) system and ultra-short pulsed electron Linac system at the Korea Atomic Energy Research Institute (KAERI). Compared with other types of BPMs, the cavity-type BPM has higher sensitivity and faster response time even at low charge levels. When electron beam passes through the cavity-type BPM, it excites the dipole mode of the cavity of which amplitude depends linearly on the beam offset from the center of the cavity. Signals from the BPM were measured as a function of the beam offset by using an oscilloscope. The microtron accelerator for the KAERI THz FEL produces the electron beam with an energy of 6.5 MeV and pulse length of 5 μs with a micropulse of 10-20 ps at the frequency of 2.801 GHz. The macropulse beam current is 40 mA. Because the microtron provides multi-bunch system, output signal would be the superposition of each single bunch. So high output signal can be obtained from superposition of each single bunch. The designed position resolution of the cavity-type BPM in multi-bunch is submicron. Our cavity-type BPM is made of aluminum and vacuum can be maintained by indium sealing without brazing process, resulting in easy modification and cost saving. The resonance frequency of the cavity-type BPM is 2.803 GHz and the cavity-type BPM dimensions are 200 × 220 mm (length × height) with a pipe diameter of 38 mm. The measured position sensitivity was 6.19 (mV/mm)/mA and the measured isolation between the X and Y axis was -39 dB. By measuring the thermal noise of system, position resolution of the cavity-type BPM was estimated to be less than 1 μm. In this article, we present the test results of the S-band cavity-type BPM and prove the feasibility of the beam position measurement with high resolution using this device.

Development of an S-band cavity-type beam position monitor for a high power THz free-electron laser

DOE Office of Scientific and Technical Information (OSTI.GOV)

Noh, Seon Yeong; Kim, Eun-San, E-mail: eskim1@knu.ac.kr; Hwang, Ji-Gwang

2015-01-15

A cavity-type beam position monitor (BPM) has been developed for a compact terahertz (THz) free-electron laser (FEL) system and ultra-short pulsed electron Linac system at the Korea Atomic Energy Research Institute (KAERI). Compared with other types of BPMs, the cavity-type BPM has higher sensitivity and faster response time even at low charge levels. When electron beam passes through the cavity-type BPM, it excites the dipole mode of the cavity of which amplitude depends linearly on the beam offset from the center of the cavity. Signals from the BPM were measured as a function of the beam offset by using anmore » oscilloscope. The microtron accelerator for the KAERI THz FEL produces the electron beam with an energy of 6.5 MeV and pulse length of 5 μs with a micropulse of 10-20 ps at the frequency of 2.801 GHz. The macropulse beam current is 40 mA. Because the microtron provides multi-bunch system, output signal would be the superposition of each single bunch. So high output signal can be obtained from superposition of each single bunch. The designed position resolution of the cavity-type BPM in multi-bunch is submicron. Our cavity-type BPM is made of aluminum and vacuum can be maintained by indium sealing without brazing process, resulting in easy modification and cost saving. The resonance frequency of the cavity-type BPM is 2.803 GHz and the cavity-type BPM dimensions are 200 × 220 mm (length × height) with a pipe diameter of 38 mm. The measured position sensitivity was 6.19 (mV/mm)/mA and the measured isolation between the X and Y axis was −39 dB. By measuring the thermal noise of system, position resolution of the cavity-type BPM was estimated to be less than 1 μm. In this article, we present the test results of the S-band cavity-type BPM and prove the feasibility of the beam position measurement with high resolution using this device.« less

Catalogue of Lygistorrhinidae (Diptera: Bibionomorpha) types housed in the collection of the Museu de Zoologia da Universidade de São Paulo, Brazil.

PubMed

Oliveira, Sarah Siqueira; Falaschi, Rafaela Lopes; Lamas, Carlos josÉ Einicker

2018-04-20

This paper provides a catalogue of the type specimens of Lygistorrhinidae (Diptera: Bibionomorpha) held in the collection of the Museu de Zoologia da Universidade de São Paulo, Brazil (MZUSP). Label information and type condition of the six type specimens (three holotypes and three paratypes) of four species are provided, along with high resolution images of the types and their labels.

High spatial resolution mapping of land cover types in a priority area for conservation in the Brazilian savanna

NASA Astrophysics Data System (ADS)

Ribeiro, F.; Roberts, D. A.; Hess, L. L.; Davis, F. W.; Caylor, K. K.; Nackoney, J.; Antunes Daldegan, G.

2017-12-01

Savannas are heterogeneous landscapes consisting of highly mixed land cover types that lack clear distinct boundaries. The Brazilian Cerrado is a Neotropical savanna considered a biodiversity hotspot for conservation due to its biodiversity richness and rapid transformation of its landscape by crop and pasture activities. The Cerrado is one of the most threatened Brazilian biomes and only 2.2% of its original extent is strictly protected. Accurate mapping and monitoring of its ecosystems and adjacent land use are important to select areas for conservation and to improve our understanding of the dynamics in this biome. Land cover mapping of savannas is difficult due to spectral similarity between land cover types resulting from similar vegetation structure, floristically similar components, generalization of land cover classes, and heterogeneity usually expressed as small patch sizes within the natural landscape. These factors are the major contributor to misclassification and low map accuracies among remote sensing studies in savannas. Specific challenges to map the Cerrado's land cover types are related to the spectral similarity between classes of land use and natural vegetation, such as natural grassland vs. cultivated pasture, and forest ecosystem vs. crops. This study seeks to classify and evaluate the land cover patterns across an area ranked as having extremely high priority for future conservation in the Cerrado. The main objective of this study is to identify the representativeness of each vegetation type across the landscape using high to moderate spatial resolution imagery using an automated scheme. A combination of pixel-based and object-based approaches were tested using RapidEye 3A imagery (5m spatial resolution) to classify the Cerrado's major land cover types. The random forest classifier was used to map the major ecosystems present across the area, and demonstrated to have an effective result with 68% of overall accuracy. Post-classification modification was performed to refine information to the major physiognomic groups of each ecosystem type. In this step, we used segmentation in eCognition, considering the random forest classification as input as well as other environmental layers (e.g. slope, soil types), which improved the overall classification to 75%.

A high-resolution angiosperm pollen reference record covering Albian mid-latitude coastal deposits (Lusitanian Basin, Portugal)

NASA Astrophysics Data System (ADS)

Horikx, Maurits; Dinis, Jorge L.; Heimhofer, Ulrich

2013-04-01

The Lusitanian Basin in Portugal is one of the most important areas to investigate the rise and radiation of early angiosperms. Here, important micro-, macro- and mesofossil remains including pollen, reproductive organs, fruits and seeds have been found. In this study, a high-resolution Early to Late Albian pollen record from a thick (~160m) coastal succession in the Lusitanian Basin containing mixed carbonate-siliciclastic near-shore deposits is generated. The outcrop is located near the town of Ericeira (São Julião) and exhibits some important new features compared to existing records from the Lusitanian basin. The comparatively proximal depositional setting and high sedimentation rate of the São Julião outcrop is well suited for high-resolution palynological sampling compared to previously studied, more distal outcrops. In addition, the succession covers almost the entire Albian including a thick interval representing Late Albian strata. Dating of the succession was obtained using dinoflagellate cyst biostratigraphy, bulk C-isotope analysis and strontium isotope analysis of low-Mg oysters and rudist shells. The high-resolution pollen record shows a distinct radiation pattern of early angiosperm pollen as well as significant changes in the accompanying palynoflora. During most of the section gymnosperm pollen types such as Classopollis spp., Inaperturopollenites spp. and Exesipollenites spp. are dominant. Angiosperm pollen abundances do not exceed 20%, although angiosperms increase slightly from the Early Albian onwards. Monoaperturate grains of magnoliid or monocot affinity remain the most dominant angiosperm pollen type, both in abundances and diversity. Tricolpate and zonoaperturate pollen grains are also present. In addition, the occurrence of several odd-shaped Dichastopollenites-type pollen types is intriguing. The palynological results indicate a warm and dry climate during most of the Albian, although a rise in the spores over pollen ratio in the second half of the section may suggest more humid conditions. In summary, the combined approach of Sr- and C-isotope stratigraphy together with marine biostratigraphy gives an accurate age assessment, which enables precise dating of the angiosperm pollen pattern emerging during the Albian.

Constraints on the Profiles of Total Water PDF in AGCMs from AIRS and a High-Resolution Model

NASA Technical Reports Server (NTRS)

Molod, Andrea

2012-01-01

Atmospheric general circulation model (AGCM) cloud parameterizations generally include an assumption about the subgrid-scale probability distribution function (PDF) of total water and its vertical profile. In the present study, the Atmospheric Infrared Sounder (AIRS) monthly-mean cloud amount and relative humidity fields are used to compute a proxy for the second moment of an AGCM total water PDF called the RH01 diagnostic, which is the AIRS mean relative humidity for cloud fractions of 0.1 or less. The dependence of the second moment on horizontal grid resolution is analyzed using results from a high-resolution global model simulation.The AIRS-derived RH01 diagnostic is generally larger near the surface than aloft, indicating a narrower PDF near the surface, and varies with the type of underlying surface. High-resolution model results show that the vertical structure of profiles of the AGCM PDF second moment is unchanged as the grid resolution changes from 200 to 100 to 50 km, and that the second-moment profiles shift toward higher values with decreasing grid spacing.Several Goddard Earth Observing System, version 5 (GEOS-5), AGCM simulations were performed with several choices for the profile of the PDF second moment. The resulting cloud and relative humidity fields were shown to be quite sensitive to the prescribed profile, and the use of a profile based on the AIRS-derived proxy results in improvements relative to observational estimates. The AIRS-guided total water PDF profiles, including their dependence on underlying surface type and on horizontal resolution, have been implemented in the version of the GEOS-5 AGCM used for publicly released simulations.

Cavity beam position monitor system for the Accelerator Test Facility 2

NASA Astrophysics Data System (ADS)

Kim, Y. I.; Ainsworth, R.; Aryshev, A.; Boogert, S. T.; Boorman, G.; Frisch, J.; Heo, A.; Honda, Y.; Hwang, W. H.; Huang, J. Y.; Kim, E.-S.; Kim, S. H.; Lyapin, A.; Naito, T.; May, J.; McCormick, D.; Mellor, R. E.; Molloy, S.; Nelson, J.; Park, S. J.; Park, Y. J.; Ross, M.; Shin, S.; Swinson, C.; Smith, T.; Terunuma, N.; Tauchi, T.; Urakawa, J.; White, G. R.

2012-04-01

The Accelerator Test Facility 2 (ATF2) is a scaled demonstrator system for final focus beam lines of linear high energy colliders. This paper describes the high resolution cavity beam position monitor (BPM) system, which is a part of the ATF2 diagnostics. Two types of cavity BPMs are used, C-band operating at 6.423 GHz, and S-band at 2.888 GHz with an increased beam aperture. The cavities, electronics, and digital processing are described. The resolution of the C-band system with attenuators was determined to be approximately 250 nm and 1μm for the S-band system. Without attenuation the best recorded C-band cavity resolution was 27 nm.

Aircraft Detection in High-Resolution SAR Images Based on a Gradient Textural Saliency Map.

PubMed

Tan, Yihua; Li, Qingyun; Li, Yansheng; Tian, Jinwen

2015-09-11

This paper proposes a new automatic and adaptive aircraft target detection algorithm in high-resolution synthetic aperture radar (SAR) images of airport. The proposed method is based on gradient textural saliency map under the contextual cues of apron area. Firstly, the candidate regions with the possible existence of airport are detected from the apron area. Secondly, directional local gradient distribution detector is used to obtain a gradient textural saliency map in the favor of the candidate regions. In addition, the final targets will be detected by segmenting the saliency map using CFAR-type algorithm. The real high-resolution airborne SAR image data is used to verify the proposed algorithm. The results demonstrate that this algorithm can detect aircraft targets quickly and accurately, and decrease the false alarm rate.

P-CSI v1.0, an accelerated barotropic solver for the high-resolution ocean model component in the Community Earth System Model v2.0

NASA Astrophysics Data System (ADS)

Huang, Xiaomeng; Tang, Qiang; Tseng, Yuheng; Hu, Yong; Baker, Allison H.; Bryan, Frank O.; Dennis, John; Fu, Haohuan; Yang, Guangwen

2016-11-01

In the Community Earth System Model (CESM), the ocean model is computationally expensive for high-resolution grids and is often the least scalable component for high-resolution production experiments. The major bottleneck is that the barotropic solver scales poorly at high core counts. We design a new barotropic solver to accelerate the high-resolution ocean simulation. The novel solver adopts a Chebyshev-type iterative method to reduce the global communication cost in conjunction with an effective block preconditioner to further reduce the iterations. The algorithm and its computational complexity are theoretically analyzed and compared with other existing methods. We confirm the significant reduction of the global communication time with a competitive convergence rate using a series of idealized tests. Numerical experiments using the CESM 0.1° global ocean model show that the proposed approach results in a factor of 1.7 speed-up over the original method with no loss of accuracy, achieving 10.5 simulated years per wall-clock day on 16 875 cores.

Microlaser-based displays

NASA Astrophysics Data System (ADS)

Bergstedt, Robert; Fink, Charles G.; Flint, Graham W.; Hargis, David E.; Peppler, Philipp W.

1997-07-01

Laser Power Corporation has developed a new type of projection display, based upon microlaser technology and a novel scan architecture, which provides the foundation for bright, extremely high resolution images. A review of projection technologies is presented along with the limitations of each and the difficulties they experience in trying to generate high resolution imagery. The design of the microlaser based projector is discussed along with the advantage of this technology. High power red, green, and blue microlasers have been designed and developed specifically for use in projection displays. These sources, in combination with high resolution, high contrast modulator, produce a 24 bit color gamut, capable of supporting the full range of real world colors. The new scan architecture, which reduces the modulation rate and scan speeds required, is described. This scan architecture, along with the inherent brightness of the laser provides the fundamentals necessary to produce a 5120 by 4096 resolution display. The brightness and color uniformity of the display is excellent, allowing for tiling of the displays with far fewer artifacts than those in a traditionally tiled display. Applications for the display include simulators, command and control centers, and electronic cinema.

Modeling Above-Ground Biomass Across Multiple Circum-Arctic Tundra Sites Using High Spatial Resolution Remote Sensing

NASA Astrophysics Data System (ADS)

Räsänen, Aleksi; Juutinen, Sari; Aurela, Mika; Virtanen, Tarmo

2017-04-01

Biomass is one of the central bio-geophysical variables in Earth observation for tracking plant productivity, and flow of carbon, nutrients, and water. Most of the satellite based biomass mapping exercises in Arctic environments have been performed by using rather coarse spatial resolution data, e.g. Landsat and AVHRR which have spatial resolutions of 30 m and >1 km, respectively. While the coarse resolution images have high temporal resolution, they are incapable of capturing the fragmented nature of tundra environment and fine-scale changes in vegetation and carbon exchange patterns. Very high spatial resolution (VHSR, spatial resolution 0.5-2 m) satellite images have the potential to detect environmental variables with an ecologically sound spatial resolution. The usage of VHSR images has, nevertheless, been modest so far in biomass modeling in the Arctic. Our objectives were to use VHSR for predicting above ground biomass in tundra landscapes, evaluate whether a common predictive model can be applied across circum-Arctic tundra and peatland sites having different types of vegetation, and produce knowledge on distribution of plant functional types (PFT) in these sites. Such model development is dependent on ground-based surveys of vegetation with the same spatial resolution and extent with the VHSR images. In this study, we conducted ground-based surveys of vegetation composition and biomass in four different arctic tundra or peatland areas located in Russia, Canada, and Finland. First, we sorted species into PFTs and developed PFT-specific models to predict biomass on the basis of non-destructive measurements (cover, height). Second, we predicted overall biomass on landscape scale by combinations of single bands and vegetation indices of very high resolution satellite images (QuickBird or WorldView-2 images of the eight sites). We compared area-specific empirical regression models and common models that were applied across all sites. We found that NDVI was usually the highest scoring spectral indices in explaining biomass distribution with good explanatory power. Furthermore, models which had more than one explanatory variable had higher explanatory power than models with a single index. The dissimilarity between common and site-specific model estimates was, however, high and data indicates that variation in vegetation properties and its impact on spectral reflectance needs to be acknowledged. Our work produced knowledge on above-ground biomass distribution and contribution of PFTs across circum-Arctic low-growth landscapes and will contribute to developing space-borne vegetation monitoring schemes utilizing VHSR satellite images.

High-resolution manometry findings in patients with achalasia and massive dilated megaesophagus.

PubMed

Menezes, M A; Andolfi, C; Herbella, F A M; Patti, M G

2017-05-01

Achalasia may present in a non-advanced or an advanced (end stage) stage based on the degree of esophageal dilatation. Manometric parameters and esophageal caliber may be prognostic for the outcome of treatment. The correlation between manometry and disease stage has not been yet fully studied. This study aims to describe high-resolution manometry findings in patients with achalasia and massive dilated megaesophagus. Eighteen patients (mean age 61 years, 55% females) with achalasia and massive dilated megaesophagus, as defined by a maximum esophageal dilatation >10 cm at the barium esophagram, were studied. Achalasia was considered secondary to Chagas' disease in 14 (78%) of the patients and idiopathic in the remaining. All patients underwent high-resolution manometry. Upper esophageal sphincter was hypotonic and had impaired relaxation in the majority of patients. Aperistalsis was seen in all patients with an equal distribution of Chicago type I and type II. No type III was noticed. Lower esophageal sphincter did not have a characteristic manometric pattern. In 50% of the cases, the manometry catheter was not able to reach the stomach. Our results did not show a manometric pattern in patients with achalasia and massive dilated esophagus. © The Authors 2017. Published by Oxford University Press on behalf of International Society for Diseases of the Esophagus. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.

Impacts of future changes in weather condition on U.S. transportation

NASA Astrophysics Data System (ADS)

Ashfaq, M.; Pagan, B. R.; Bonds, B. W.; Rastogi, D.

2016-12-01

High-resolution near-term climate projections suggest an intensification of the regional hydrological cycle over the U.S., leading to stronger and more frequent precipitation events. Increase in precipitation extremes is driven by both warm season convection driven rainstorms and frontal based cold season snowstorms. Results also indicate that future warming is driven more by hot extremes, as decrease in cold extremes is three times less than increase in hot extremes. While projected changes may likely impact the transportation system across the U.S., accurate estimation of such impacts requires knowledge of changes in precipitation types (rain, snow, ice, freezing rain). Here we apply four commonly used precipitation typing algorithms to determine different types of precipitation in an 11-memebr high-resolution (18 km) climate projections dataset that covers 40 years (1966-2005) in the baseline and 40 years (2011-2050) in the future period under Representative Concentration Pathway 8.5. The results are compared with the NARR-based precipitation classification in the historical period at the county level. Documented weather related county level fatal crash data for the CONUS and non-fatal crash data for selected states in the eastern half of the U.S. is compiled to develop the historical baseline for the impact of weather conditions on transportation. Further analysis is carried out to understand the ability of an ensemble of high-resolution simulations to produce different precipitation types in the baseline period, potential changes in the occurrence of each type of weather condition in the future period and that how such changes may impact road conditions, vehicle crashes and human fatalities. Additional analysis will also be explored to understand the impact of changes in winter weather conditions on the cost associated with road maintenance.

Dynamic frequency-domain interferometer for absolute distance measurements with high resolution

NASA Astrophysics Data System (ADS)

Weng, Jidong; Liu, Shenggang; Ma, Heli; Tao, Tianjiong; Wang, Xiang; Liu, Cangli; Tan, Hua

2014-11-01

A unique dynamic frequency-domain interferometer for absolute distance measurement has been developed recently. This paper presents the working principle of the new interferometric system, which uses a photonic crystal fiber to transmit the wide-spectrum light beams and a high-speed streak camera or frame camera to record the interference stripes. Preliminary measurements of harmonic vibrations of a speaker, driven by a radio, and the changes in the tip clearance of a rotating gear wheel show that this new type of interferometer has the ability to perform absolute distance measurements both with high time- and distance-resolution.

Quantitative Evaluation of PET Respiratory Motion Correction Using MR Derived Simulated Data

NASA Astrophysics Data System (ADS)

Polycarpou, Irene; Tsoumpas, Charalampos; King, Andrew P.; Marsden, Paul K.

2015-12-01

The impact of respiratory motion correction on quantitative accuracy in PET imaging is evaluated using simulations for variable patient specific characteristics such as tumor uptake and respiratory pattern. Respiratory patterns from real patients were acquired, with long quiescent motion periods (type-1) as commonly observed in most patients and with long-term amplitude variability as is expected under conditions of difficult breathing (type-2). The respiratory patterns were combined with an MR-derived motion model to simulate real-time 4-D PET-MR datasets. Lung and liver tumors were simulated with diameters of 10 and 12 mm and tumor-to-background ratio ranging from 3:1 to 6:1. Projection data for 6- and 3-mm PET resolution were generated for the Philips Gemini scanner and reconstructed without and with motion correction using OSEM (2 iterations, 23 subsets). Motion correction was incorporated into the reconstruction process based on MR-derived motion fields. Tumor peak standardized uptake values (SUVpeak) were calculated from 30 noise realizations. Respiratory motion correction improves the quantitative performance with the greatest benefit observed for patients of breathing type-2. For breathing type-1 after applying motion correction, SUVpeak of 12-mm liver tumor with 6:1 contrast was increased by 46% for a current PET resolution (i.e., 6 mm) and by 47% for a higher PET resolution (i.e., 3 mm). Furthermore, the results of this study indicate that the benefit of higher scanner resolution is small unless motion correction is applied. In particular, for large liver tumor (12 mm) with low contrast (3:1) after motion correction, the SUVpeak was increased by 34% for 6-mm resolution and by 50% for a higher PET resolution (i.e., 3-mm resolution. This investigation indicates that there is a high impact of respiratory motion correction on tumor quantitative accuracy and that motion correction is important in order to benefit from the increased resolution of future PET scanners.

[Polymorphism of HLA-B* 40 gene family in Chinese Han population].

PubMed

Li, Zhen; Jin, Shi-Zheng; Cheng, Liang-Hong; Wang, Da-Ming; Zhou, Dan; Zou, Hong-Yan; Wu, Guo-Guang

2005-04-01

To investigate the allele distribution of HLA-B* 40 gene family in Chinese Han population and to study its influence on the selection of clinical transplantation donor, the HLA-B genetypes of 381 individuals randomly selected from Chinese National Marrow Donor Project were identified by PCR-SSO, and then all the HLA-B* 40 positive samples from the above population and the B* 40 homozygote samples received from another 1 270 registered donors were analyzed by PCR-SBT and PCR-SSP at high resolution. The results showed that the population of 381 registered donors was examined at HLA-B locus by using Hardy-Weinberg equilibrium, the gene frequency of HLA-B* 40 was 0.1692. Four different HLA-B* 40 alleles (B* 4001, B* 4002, B* 4003, B* 4006) were identified, and the serological specificity was B60 and B61 respectively. The relative frequency of each allele was 0.1192 for B* 4001, 0.0154 for B* 4002, 0.0038 for B* 4003, 0.0308 for B* 4006. The distribution of B* 40 homozygote revealed a certain regularity at high-resolution, B* 40XX (B* 4001 group), at low-resolution; B* 4001 at high resolution; B* 40XX (B* 4002 group), at low-resolution; B* 4002 or B* 4006 or heterozygote of both at high-resolution. It is concluded that in Chinese Han population, predominant allele in HLA-B* 40 gene family is B* 4001, the high-resolution typing may be recommended to use for the selection of clinical transplantation donor.

A 3D CZT high resolution detector for x- and gamma-ray astronomy

NASA Astrophysics Data System (ADS)

Kuvvetli, I.; Budtz-Jørgensen, C.; Zappettini, A.; Zambelli, N.; Benassi, G.; Kalemci, E.; Caroli, E.; Stephen, J. B.; Auricchio, N.

2014-07-01

At DTU Space we have developed a high resolution three dimensional (3D) position sensitive CZT detector for high energy astronomy. The design of the 3D CZT detector is based on the CZT Drift Strip detector principle. The position determination perpendicular to the anode strips is performed using a novel interpolating technique based on the drift strip signals. The position determination in the detector depth direction, is made using the DOI technique based the detector cathode and anode signals. The position determination along the anode strips is made with the help of 10 cathode strips orthogonal to the anode strips. The position resolutions are at low energies dominated by the electronic noise and improve therefore with increased signal to noise ratio as the energy increases. The achievable position resolution at higher energies will however be dominated by the extended spatial distribution of the photon produced ionization charge. The main sources of noise contribution of the drift signals are the leakage current between the strips and the strip capacitance. For the leakage current, we used a metallization process that reduces the leakage current by means of a high resistive thin layer between the drift strip electrodes and CZT detector material. This method was applied to all the proto type detectors and was a very effective method to reduce the surface leakage current between the strips. The proto type detector was recently investigated at the European Synchrotron Radiation Facility, Grenoble which provided a fine 50 × 50 μm2 collimated X-ray beam covering an energy band up to 600 keV. The Beam positions are resolved very well with a ~ 0.2 mm position resolution (FWHM ) at 400 keV in all directions.

Fiber Optic Distributed Sensors for High-resolution Temperature Field Mapping.

PubMed

Lomperski, Stephen; Gerardi, Craig; Lisowski, Darius

2016-11-07

The reliability of computational fluid dynamics (CFD) codes is checked by comparing simulations with experimental data. A typical data set consists chiefly of velocity and temperature readings, both ideally having high spatial and temporal resolution to facilitate rigorous code validation. While high resolution velocity data is readily obtained through optical measurement techniques such as particle image velocimetry, it has proven difficult to obtain temperature data with similar resolution. Traditional sensors such as thermocouples cannot fill this role, but the recent development of distributed sensing based on Rayleigh scattering and swept-wave interferometry offers resolution suitable for CFD code validation work. Thousands of temperature measurements can be generated along a single thin optical fiber at hundreds of Hertz. Sensors function over large temperature ranges and within opaque fluids where optical techniques are unsuitable. But this type of sensor is sensitive to strain and humidity as well as temperature and so accuracy is affected by handling, vibration, and shifts in relative humidity. Such behavior is quite unlike traditional sensors and so unconventional installation and operating procedures are necessary to ensure accurate measurements. This paper demonstrates implementation of a Rayleigh scattering-type distributed temperature sensor in a thermal mixing experiment involving two air jets at 25 and 45 °C. We present criteria to guide selection of optical fiber for the sensor and describe installation setup for a jet mixing experiment. We illustrate sensor baselining, which links readings to an absolute temperature standard, and discuss practical issues such as errors due to flow-induced vibration. This material can aid those interested in temperature measurements having high data density and bandwidth for fluid dynamics experiments and similar applications. We highlight pitfalls specific to these sensors for consideration in experiment design and operation.

Multilocus sequence typing (MLST) for lineage assignment and high resolution diversity studies in Trypanosoma cruzi.

PubMed

Yeo, Matthew; Mauricio, Isabel L; Messenger, Louisa A; Lewis, Michael D; Llewellyn, Martin S; Acosta, Nidia; Bhattacharyya, Tapan; Diosque, Patricio; Carrasco, Hernan J; Miles, Michael A

2011-06-01

Multilocus sequence typing (MLST) is a powerful and highly discriminatory method for analysing pathogen population structure and epidemiology. Trypanosoma cruzi, the protozoan agent of American trypanosomiasis (Chagas disease), has remarkable genetic and ecological diversity. A standardised MLST protocol that is suitable for assignment of T. cruzi isolates to genetic lineage and for higher resolution diversity studies has not been developed. We have sequenced and diplotyped nine single copy housekeeping genes and assessed their value as part of a systematic MLST scheme for T. cruzi. A minimum panel of four MLST targets (Met-III, RB19, TcGPXII, and DHFR-TS) was shown to provide unambiguous assignment of isolates to the six known T. cruzi lineages (Discrete Typing Units, DTUs TcI-TcVI). In addition, we recommend six MLST targets (Met-II, Met-III, RB19, TcMPX, DHFR-TS, and TR) for more in depth diversity studies on the basis that diploid sequence typing (DST) with this expanded panel distinguished 38 out of 39 reference isolates. Phylogenetic analysis implies a subdivision between North and South American TcIV isolates. Single Nucleotide Polymorphism (SNP) data revealed high levels of heterozygosity among DTUs TcI, TcIII, TcIV and, for three targets, putative corresponding homozygous and heterozygous loci within DTUs TcI and TcIII. Furthermore, individual gene trees gave incongruent topologies at inter- and intra-DTU levels, inconsistent with a model of strict clonality. We demonstrate the value of systematic MLST diplotyping for describing inter-DTU relationships and for higher resolution diversity studies of T. cruzi, including presence of recombination events. The high levels of heterozygosity will facilitate future population genetics analysis based on MLST haplotypes.

Definition of a temporal distribution index for high temporal resolution precipitation data over Peninsular Spain and the Balearic Islands: the fractal dimension; and its synoptic implications

NASA Astrophysics Data System (ADS)

Meseguer-Ruiz, Oliver; Osborn, Timothy J.; Sarricolea, Pablo; Jones, Philip D.; Cantos, Jorge Olcina; Serrano-Notivoli, Roberto; Martin-Vide, Javier

2018-03-01

Precipitation on the Spanish mainland and in the Balearic archipelago exhibits a high degree of spatial and temporal variability, regardless of the temporal resolution of the data considered. The fractal dimension indicates the property of self-similarity, and in the case of this study, wherein it is applied to the temporal behaviour of rainfall at a fine (10-min) resolution from a total of 48 observatories, it provides insights into its more or less convective nature. The methodology of Jenkinson & Collison which automatically classifies synoptic situations at the surface, as well as an adaptation of this methodology at 500 hPa, was applied in order to gain insights into the synoptic implications of extreme values of the fractal dimension. The highest fractal dimension values in the study area were observed in places with precipitation that has a more random behaviour over time with generally high totals. Four different regions in which the atmospheric mechanisms giving rise to precipitation at the surface differ from the corresponding above-ground mechanisms have been identified in the study area based on the fractal dimension. In the north of the Iberian Peninsula, high fractal dimension values are linked to a lower frequency of anticyclonic situations, whereas the opposite occurs in the central region. In the Mediterranean, higher fractal dimension values are associated with a higher frequency of the anticyclonic type and a lower frequency of the advective type from the east. In the south, lower fractal dimension values indicate higher frequency with respect to the anticyclonic type from the east and lower frequency with respect to the cyclonic type.

Fast fMRI provides high statistical power in the analysis of epileptic networks.

PubMed

Jacobs, Julia; Stich, Julia; Zahneisen, Benjamin; Assländer, Jakob; Ramantani, Georgia; Schulze-Bonhage, Andreas; Korinthenberg, Rudolph; Hennig, Jürgen; LeVan, Pierre

2014-03-01

EEG-fMRI is a unique method to combine the high temporal resolution of EEG with the high spatial resolution of MRI to study generators of intrinsic brain signals such as sleep grapho-elements or epileptic spikes. While the standard EPI sequence in fMRI experiments has a temporal resolution of around 2.5-3s a newly established fast fMRI sequence called MREG (Magnetic-Resonance-Encephalography) provides a temporal resolution of around 100ms. This technical novelty promises to improve statistics, facilitate correction of physiological artifacts and improve the understanding of epileptic networks in fMRI. The present study compares simultaneous EEG-EPI and EEG-MREG analyzing epileptic spikes to determine the yield of fast MRI in the analysis of intrinsic brain signals. Patients with frequent interictal spikes (>3/20min) underwent EEG-MREG and EEG-EPI (3T, 20min each, voxel size 3×3×3mm, EPI TR=2.61s, MREG TR=0.1s). Timings of the spikes were used in an event-related analysis to generate activation maps of t-statistics. (FMRISTAT, |t|>3.5, cluster size: 7 voxels, p<0.05 corrected). For both sequences, the amplitude and location of significant BOLD activations were compared with the spike topography. 13 patients were recorded and 33 different spike types could be analyzed. Peak T-values were significantly higher in MREG than in EPI (p<0.0001). Positive BOLD effects correlating with the spike topography were found in 8/29 spike types using the EPI and in 22/33 spikes types using the MREG sequence. Negative BOLD responses in the default mode network could be observed in 3/29 spike types with the EPI and in 19/33 with the MREG sequence. With the latter method, BOLD changes were observed even when few spikes occurred during the investigation. Simultaneous EEG-MREG thus is possible with good EEG quality and shows higher sensitivity in regard to the localization of spike-related BOLD responses than EEG-EPI. The development of new methods of analysis for this sequence such as modeling of physiological noise, temporal analysis of the BOLD signal and defining appropriate thresholds is required to fully profit from its high temporal resolution. © 2013.

Near-Infrared Continuum and 3.3um PAH Imaging of the Starburst Ring in the Type I Seyfert Galaxy NGC 7469

NASA Technical Reports Server (NTRS)

Mazzarella, J.; Voit, G.; Soifer, B.; Matthews, K.; Graham, J.; Armus, L.; Shupe, D.

1993-01-01

High resolution near-infrared images of the type 1 Seyfert Galaxy NGC 7469 have been obtained to probe its dusty nuclear environment. Direct images are relatively featureless, but residual images created by subtacting a smooth model based on best-fitting elliptical isoophotes reveal a tight inner spiral whose high surface-brightness portions correspond to a previously detected 3.

Push-Broom-Type Very High-Resolution Satellite Sensor Data Correction Using Combined Wavelet-Fourier and Multiscale Non-Local Means Filtering.

PubMed

Kang, Wonseok; Yu, Soohwan; Seo, Doochun; Jeong, Jaeheon; Paik, Joonki

2015-09-10

In very high-resolution (VHR) push-broom-type satellite sensor data, both destriping and denoising methods have become chronic problems and attracted major research advances in the remote sensing fields. Since the estimation of the original image from a noisy input is an ill-posed problem, a simple noise removal algorithm cannot preserve the radiometric integrity of satellite data. To solve these problems, we present a novel method to correct VHR data acquired by a push-broom-type sensor by combining wavelet-Fourier and multiscale non-local means (NLM) filters. After the wavelet-Fourier filter separates the stripe noise from the mixed noise in the wavelet low- and selected high-frequency sub-bands, random noise is removed using the multiscale NLM filter in both low- and high-frequency sub-bands without loss of image detail. The performance of the proposed method is compared to various existing methods on a set of push-broom-type sensor data acquired by Korean Multi-Purpose Satellite 3 (KOMPSAT-3) with severe stripe and random noise, and the results of the proposed method show significantly improved enhancement results over existing state-of-the-art methods in terms of both qualitative and quantitative assessments.

Push-Broom-Type Very High-Resolution Satellite Sensor Data Correction Using Combined Wavelet-Fourier and Multiscale Non-Local Means Filtering

PubMed Central

Kang, Wonseok; Yu, Soohwan; Seo, Doochun; Jeong, Jaeheon; Paik, Joonki

2015-01-01

In very high-resolution (VHR) push-broom-type satellite sensor data, both destriping and denoising methods have become chronic problems and attracted major research advances in the remote sensing fields. Since the estimation of the original image from a noisy input is an ill-posed problem, a simple noise removal algorithm cannot preserve the radiometric integrity of satellite data. To solve these problems, we present a novel method to correct VHR data acquired by a push-broom-type sensor by combining wavelet-Fourier and multiscale non-local means (NLM) filters. After the wavelet-Fourier filter separates the stripe noise from the mixed noise in the wavelet low- and selected high-frequency sub-bands, random noise is removed using the multiscale NLM filter in both low- and high-frequency sub-bands without loss of image detail. The performance of the proposed method is compared to various existing methods on a set of push-broom-type sensor data acquired by Korean Multi-Purpose Satellite 3 (KOMPSAT-3) with severe stripe and random noise, and the results of the proposed method show significantly improved enhancement results over existing state-of-the-art methods in terms of both qualitative and quantitative assessments. PMID:26378532

First High Resolution IR Study of the νb{14} (a') A-Type Band Near 421.847 \\wn of 2-^{13}C-PROPENE

NASA Astrophysics Data System (ADS)

Daunt, S. J.; Grzywacz, Robert; Billinghurst, Brant E.

2017-06-01

This is is the first high resolution IR study of any band of the 2-^{13}C-propene species. There have been only two previous high resolution studies of vibration-rotation bands of the normal species. The band examined here is the νb{14} (A') CCC skeletal bending near 421.847 \\wn which has an A-Type asymmetric rotor structure. The spectra were recorded on the FTS at the Far-IR beamline of the Canadian Light Source with a resolution of Δν = 0.0009 \\wn. We have assigned and fitted around 2200 transitions and determined ground and upper state rotational constants. Lines with J up to 49 and K up to 12 were included. The subbands with K greater than 12 were perturbed and show torsional splittings that vary from small to extremely large. The fitting was done with the PGOPHER program of Colin Western. The GS constants are in good agreement with the MW constants reported recently by Craig, Groner and co-workers. Ainetschian, Fraser, Ortigoso & Pate, J. Chem. Phys. 100, 729 ff. (1994); Lafferty, Flaud & Herman, J. Mol. Struct. 780-781, 65 ff. (2006). Western, J. Quant. Spectrosc. Rad. Transf. 186, 221 ff. (2017). Paper M109, 71st ISMS Symposium (2016); J. Mol. Spectrosc. 328, 1-6 (2016).

High resolution flow field prediction for tail rotor aeroacoustics

NASA Technical Reports Server (NTRS)

Quackenbush, Todd R.; Bliss, Donald B.

1989-01-01

The prediction of tail rotor noise due to the impingement of the main rotor wake poses a significant challenge to current analysis methods in rotorcraft aeroacoustics. This paper describes the development of a new treatment of the tail rotor aerodynamic environment that permits highly accurate resolution of the incident flow field with modest computational effort relative to alternative models. The new approach incorporates an advanced full-span free wake model of the main rotor in a scheme which reconstructs high-resolution flow solutions from preliminary, computationally inexpensive simulations with coarse resolution. The heart of the approach is a novel method for using local velocity correction terms to capture the steep velocity gradients characteristic of the vortex-dominated incident flow. Sample calculations have been undertaken to examine the principal types of interactions between the tail rotor and the main rotor wake and to examine the performance of the new method. The results of these sample problems confirm the success of this approach in capturing the high-resolution flows necessary for analysis of rotor-wake/rotor interactions with dramatically reduced computational cost. Computations of radiated sound are also carried out that explore the role of various portions of the main rotor wake in generating tail rotor noise.

Design principles and applications of a cooled CCD camera for electron microscopy.

PubMed

Faruqi, A R

1998-01-01

Cooled CCD cameras offer a number of advantages in recording electron microscope images with CCDs rather than film which include: immediate availability of the image in a digital format suitable for further computer processing, high dynamic range, excellent linearity and a high detective quantum efficiency for recording electrons. In one important respect however, film has superior properties: the spatial resolution of CCD detectors tested so far (in terms of point spread function or modulation transfer function) are inferior to film and a great deal of our effort has been spent in designing detectors with improved spatial resolution. Various instrumental contributions to spatial resolution have been analysed and in this paper we discuss the contribution of the phosphor-fibre optics system in this measurement. We have evaluated the performance of a number of detector components and parameters, e.g. different phosphors (and a scintillator), optical coupling with lens or fibre optics with various demagnification factors, to improve the detector performance. The camera described in this paper, which is based on this analysis, uses a tapered fibre optics coupling between the phosphor and the CCD and is installed on a Philips CM12 electron microscope equipped to perform cryo-microscopy. The main use of the camera so far has been in recording electron diffraction patterns from two dimensional crystals of bacteriorhodopsin--from wild type and from different trapped states during the photocycle. As one example of the type of data obtained with the CCD camera a two dimensional Fourier projection map from the trapped O-state is also included. With faster computers, it will soon be possible to undertake this type of work on an on-line basis. Also, with improvements in detector size and resolution, CCD detectors, already ideal for diffraction, will be able to compete with film in the recording of high resolution images.

Broad Separation of Isomeric Lipids by High-Resolution Differential Ion Mobility Spectrometry with Tandem Mass Spectrometry

NASA Astrophysics Data System (ADS)

Bowman, Andrew P.; Abzalimov, Rinat R.; Shvartsburg, Alexandre A.

2017-08-01

Maturation of metabolomics has brought a deeper appreciation for the importance of isomeric identity of lipids to their biological role, mirroring that for proteoforms in proteomics. However, full characterization of the lipid isomerism has been thwarted by paucity of rapid and effective analytical tools. A novel approach is ion mobility spectrometry (IMS) and particularly differential or field asymmetric waveform IMS (FAIMS) at high electric fields, which is more orthogonal to mass spectrometry. Here we broadly explore the power of FAIMS to separate lipid isomers, and find a 75% success rate across the four major types of glycero- and phospho- lipids ( sn, chain length, double bond position, and cis/ trans). The resolved isomers were identified using standards, and (for the first two types) tandem mass spectrometry. These results demonstrate the general merit of incorporating high-resolution FAIMS into lipidomic analyses.

SAPHIRE (scintillator avalanche photoconductor with high resolution emitter readout) for low dose x-ray imaging: Spatial resolution

DOE Office of Scientific and Technical Information (OSTI.GOV)

Li Dan; Zhao Wei

2008-07-15

An indirect flat panel imager (FPI) with programmable avalanche gain and field emitter array (FEA) readout is being investigated for low-dose and high resolution x-ray imaging. It is made by optically coupling a structured x-ray scintillator, e.g., thallium (Tl) doped cesium iodide (CsI), to an amorphous selenium (a-Se) avalanche photoconductor called high-gain avalanche rushing amorphous photoconductor (HARP). The charge image created by the scintillator/HARP (SHARP) combination is read out by the electron beams emitted from the FEA. The proposed detector is called scintillator avalanche photoconductor with high resolution emitter readout (SAPHIRE). The programmable avalanche gain of HARP can improve themore » low dose performance of indirect FPI while the FEA can be made with pixel sizes down to 50 {mu}m. Because of the avalanche gain, a high resolution type of CsI (Tl), which has not been widely used in indirect FPI due to its lower light output, can be used to improve the high spatial frequency performance. The purpose of the present article is to investigate the factors affecting the spatial resolution of SAPHIRE. Since the resolution performance of the SHARP combination has been well studied, the focus of the present work is on the inherent resolution of the FEA readout method. The lateral spread of the electron beam emitted from a 50 {mu}mx50 {mu}m pixel FEA was investigated with two different electron-optical designs: mesh-electrode-only and electrostatic focusing. Our results showed that electrostatic focusing can limit the lateral spread of electron beams to within the pixel size of down to 50 {mu}m. Since electrostatic focusing is essentially independent of signal intensity, it will provide excellent spatial uniformity.« less

Constraining regional scale carbon budgets at the US West Coast using a high-resolution atmospheric inverse modeling approach

NASA Astrophysics Data System (ADS)

Goeckede, M.; Michalak, A. M.; Vickers, D.; Turner, D.; Law, B.

2009-04-01

The study presented is embedded within the NACP (North American Carbon Program) West Coast project ORCA2, which aims at determining the regional carbon balance of the US states Oregon, California and Washington. Our work specifically focuses on the effect of disturbance history and climate variability, aiming at improving our understanding of e.g. drought stress and stand age on carbon sources and sinks in complex terrain with fine-scale variability in land cover types. The ORCA2 atmospheric inverse modeling approach has been set up to capture flux variability on the regional scale at high temporal and spatial resolution. Atmospheric transport is simulated coupling the mesoscale model WRF (Weather Research and Forecast) with the STILT (Stochastic Time Inverted Lagrangian Transport) footprint model. This setup allows identifying sources and sinks that influence atmospheric observations with highly resolved mass transport fields and realistic turbulent mixing. Terrestrial biosphere carbon fluxes are simulated at spatial resolutions of up to 1km and subdaily timesteps, considering effects of ecoregion, land cover type and disturbance regime on the carbon budgets. Our approach assimilates high-precision atmospheric CO2 concentration measurements and eddy-covariance data from several sites throughout the model domain, as well as high-resolution remote sensing products (e.g. LandSat, MODIS) and interpolated surface meteorology (DayMet, SOGS, PRISM). We present top-down modeling results that have been optimized using Bayesian inversion, reflecting the information on regional scale carbon processes provided by the network of high-precision CO2 observations. We address the level of detail (e.g. spatial and temporal resolution) that can be resolved by top-down modeling on the regional scale, given the uncertainties introduced by various sources for model-data mismatch. Our results demonstrate the importance of accurate modeling of carbon-water coupling, with the representation of water availability and drought stress playing a dominant role to capture spatially variable CO2 exchange rates in a region characterized by strong climatic gradients.

Parsing Stem Cell Lineage Development Using High Content Image Analysis of Epigenetic Spatial Markers.

PubMed

Kim, Joseph J; Moghe, Prabhas V

2018-06-14

This unit describes a protocol for acquiring and analyzing high-content super-resolution images of human stem cell nuclei for the characterization and classification of the cell differentiation paths based on distinct patterns of epigenetic mark organization. Here, we describe the cell culture, immunocytochemical labeling, super-resolution imaging parameters, and MATLAB-based quantitative image analysis approaches for monitoring human mesenchymal stem cells (hMSCs) and human induced pluripotent stem cells (hiPSCs) as the cells differentiate towards various lineages. Although this protocol uses specific cell types as examples, this approach could be easily extended to a variety of cell types and nuclear epigenetic and mechanosensitive biomarkers that are relevant to specific cell developmental scenarios. © 2018 by John Wiley & Sons, Inc. Copyright © 2018 John Wiley & Sons, Inc.

First High Resolution IR Spectra of 2-^{13}C-PROPANE. the νb{9} B-Type Band Near 366.767 \\wn and the νb{26} C-Type Band Near 746.615 \\wn. Determination of Ground and Upper State Constants.

NASA Astrophysics Data System (ADS)

Daunt, S. J.; Grzywacz, Robert; Lafferty, Walter; Flaud, Jean-Marie; Billinghurst, Brant E.

2017-06-01

This is the first report in a project to record high resolution IR data of the ^{13}C and D substituted isotopologues of propane. In this talk we will give details on the first high resolution (Δν = 0.0009 \\wn) IR investigation of 2-^{13}C-propane. Spectra of the CCC skeletal bending mode near 336.767 \\wn (B-type) and the wagging mode near 746.615 \\wn (C-type) were recorded using the FTS on the Far-IR beamline of the Canadian Light Source (CLS). The spectra were assigned both traditionally and with the aid of the PGOPHER program of Colin Western. The only available MW data on this molecule are the six K =0 J lines from Lide. We therefore had to use the present data to determine a new set of ground state constants that included centrifugal distortion terms for this molecule. We compare these experimentally determined values with the recent ab initio values of Villa, Senent & Carvajal. Upper state constants for both bands have been found that provide a good simulation of the spectra. The hope is that this data will be useful in identifying isotopic propane lines in Titan and other astrophysical objects. C. Western, J. Quant. Spectrosc. & Rad. Transf. 186, 221 ff. (2017). Lide, J.Chem. Phys. 33, p.1514ff. (1960). Villa, Senent & Carvajal, PCCP 15, 10258 (2013).

Homogenization of Electromagnetic and Seismic Wavefields for Joint Inverse Modeling

NASA Astrophysics Data System (ADS)

Newman, G. A.; Commer, M.; Petrov, P.; Um, E. S.

2011-12-01

A significant obstacle in developing a robust joint imaging technology exploiting seismic and electromagnetic (EM) wave fields is the resolution at which these different geophysical measurements sense the subsurface. Imaging of seismic reflection data is an order of magnitude finer in resolution and scale compared to images produced with EM data. A consistent joint image of the subsurface geophysical attributes (velocity, electrical conductivity) requires/demands the different geophysical data types be similar in their resolution of the subsurface. The superior resolution of seismic data results from the fact that the energy propagates as a wave, while propagation of EM energy is diffusive and attenuates with distance. On the other hand, the complexity of the seismic wave field can be a significant problem due to high reflectivity of the subsurface and the generation of multiple scattering events. While seismic wave fields have been very useful in mapping the subsurface for energy resources, too much scattering and too many reflections can lead to difficulties in imaging and interpreting seismic data. To overcome these obstacles a formulation for joint imaging of seismic and EM wave fields is introduced, where each data type is matched in resolution. In order to accomplish this, seismic data are first transformed into the Laplace-Fourier Domain, which changes the modeling of the seismic wave field from wave propagation to diffusion. Though high frequency information (reflectivity) is lost with this transformation, several benefits follow: (1) seismic and EM data can be easily matched in resolution, governed by the same physics of diffusion, (2) standard least squares inversion works well with diffusive type problems including both transformed seismic and EM, (3) joint imaging of seismic and EM data may produce better starting velocity models critical for successful reverse time migration or full waveform imaging of seismic data (non transformed) and (4) possibilities to image across multiple scale lengths, incorporating different types of geophysical data and attributes in the process. Important numerical details of 3D seismic wave field simulation in the Laplace-Fourier domain for both acoustic and elastic cases will also be discussed.

On controlling nonlinear dissipation in high order filter methods for ideal and non-ideal MHD

NASA Technical Reports Server (NTRS)

Yee, H. C.; Sjogreen, B.

2004-01-01

The newly developed adaptive numerical dissipation control in spatially high order filter schemes for the compressible Euler and Navier-Stokes equations has been recently extended to the ideal and non-ideal magnetohydrodynamics (MHD) equations. These filter schemes are applicable to complex unsteady MHD high-speed shock/shear/turbulence problems. They also provide a natural and efficient way for the minimization of Div(B) numerical error. The adaptive numerical dissipation mechanism consists of automatic detection of different flow features as distinct sensors to signal the appropriate type and amount of numerical dissipation/filter where needed and leave the rest of the region free from numerical dissipation contamination. The numerical dissipation considered consists of high order linear dissipation for the suppression of high frequency oscillation and the nonlinear dissipative portion of high-resolution shock-capturing methods for discontinuity capturing. The applicable nonlinear dissipative portion of high-resolution shock-capturing methods is very general. The objective of this paper is to investigate the performance of three commonly used types of nonlinear numerical dissipation for both the ideal and non-ideal MHD.

a Spiral-Based Downscaling Method for Generating 30 M Time Series Image Data

NASA Astrophysics Data System (ADS)

Liu, B.; Chen, J.; Xing, H.; Wu, H.; Zhang, J.

2017-09-01

The spatial detail and updating frequency of land cover data are important factors influencing land surface dynamic monitoring applications in high spatial resolution scale. However, the fragmentized patches and seasonal variable of some land cover types (e. g. small crop field, wetland) make it labor-intensive and difficult in the generation of land cover data. Utilizing the high spatial resolution multi-temporal image data is a possible solution. Unfortunately, the spatial and temporal resolution of available remote sensing data like Landsat or MODIS datasets can hardly satisfy the minimum mapping unit and frequency of current land cover mapping / updating at the same time. The generation of high resolution time series may be a compromise to cover the shortage in land cover updating process. One of popular way is to downscale multi-temporal MODIS data with other high spatial resolution auxiliary data like Landsat. But the usual manner of downscaling pixel based on a window may lead to the underdetermined problem in heterogeneous area, result in the uncertainty of some high spatial resolution pixels. Therefore, the downscaled multi-temporal data can hardly reach high spatial resolution as Landsat data. A spiral based method was introduced to downscale low spatial and high temporal resolution image data to high spatial and high temporal resolution image data. By the way of searching the similar pixels around the adjacent region based on the spiral, the pixel set was made up in the adjacent region pixel by pixel. The underdetermined problem is prevented to a large extent from solving the linear system when adopting the pixel set constructed. With the help of ordinary least squares, the method inverted the endmember values of linear system. The high spatial resolution image was reconstructed on the basis of high spatial resolution class map and the endmember values band by band. Then, the high spatial resolution time series was formed with these high spatial resolution images image by image. Simulated experiment and remote sensing image downscaling experiment were conducted. In simulated experiment, the 30 meters class map dataset Globeland30 was adopted to investigate the effect on avoid the underdetermined problem in downscaling procedure and a comparison between spiral and window was conducted. Further, the MODIS NDVI and Landsat image data was adopted to generate the 30m time series NDVI in remote sensing image downscaling experiment. Simulated experiment results showed that the proposed method had a robust performance in downscaling pixel in heterogeneous region and indicated that it was superior to the traditional window-based methods. The high resolution time series generated may be a benefit to the mapping and updating of land cover data.

Micromachined PIN-PMN-PT Crystal Composite Transducer for High-Frequency Intravascular Ultrasound (IVUS) Imaging

PubMed Central

Li, Xiang; Ma, Teng; Tian, Jian; Han, Pengdi; Zhou, Qifa; Shung, K. Kirk

2015-01-01

In this paper, we report the use of micromachined PbIn1/2Nb1/2O3–PbMg1/3Nb2/3O3–PbTiO3 (PIN-PMN-PT) single crystal 1–3 composite material for intravascular ultrasound (IVUS) imaging application. The effective electromechanical coupling coefficient kt(eff) of the composite was measured to be 0.75 to 0.78. Acoustic impedance was estimated to be 20 MRayl. Based on the composite, needle-type and flexible-type IVUS transducers were fabricated. The composite transducer achieved an 86% bandwidth at the center frequency of 41 MHz, which resulted in a 43 μm axial resolution. Ex vivo IVUS imaging was conducted to demonstrate the improvement of axial resolution. The composite transducer was capable of identifying the three layers of a cadaver coronary artery specimen with high resolution. The PIN-PMN-PT-based composite has superior piezoelectric properties comparable to PMN-PT-based composite and its thermal stability is higher than PMN-PT. PIN-PMN-PT crystal can be an alternative approach for fabricating high-frequency composite, instead of using PMN-PT. PMID:24960706

Increasing the electron-transfer ability of Cyanidioschyzon merolae ferredoxin by a one-point mutation – A high resolution and Fe-SAD phasing crystal structure analysis of the Asp58Asn mutant

DOE Office of Scientific and Technical Information (OSTI.GOV)

Ueno, Yuko; Matsumoto, Takashi; Yamano, Akihito

2013-07-12

Highlights: •A single amino acid change on the ferredoxin surface affects electron transfer. •Precise positions of amide atoms were located utilizing no prior structural data. •Ultra high resolution and SAD phasing may be used for bias-free model building. -- Abstract: Cyanidioschyzon merolae (Cm) is a single cell red algae that grows in rather thermophilic (40–50 °C) and acidic (pH 1–3) conditions. Ferredoxin (Fd) was purified from this algae and characterized as a plant-type [2Fe–2S] Fd by physicochemical techniques. A high resolution (0.97 Å) three-dimensional structure of the CmFd D58N mutant molecule has been determined using the Fe-SAD phasing method tomore » clarify the precise position of the Asn58 amide, as this substitution increases the electron-transfer ability relative to wild-type CmFd by a factor of 1.5. The crystal structure reveals an electro-positive surface surrounding Asn58 that may interact with ferredoxin NADP{sup +} reductase or cytochrome c.« less

High resolution Physio-chemical Tissue Analysis: Towards Non-invasive In Vivo Biopsy

NASA Astrophysics Data System (ADS)

Xu, Guan; Meng, Zhuo-Xian; Lin, Jian-Die; Deng, Cheri X.; Carson, Paul L.; Fowlkes, J. Brian; Tao, Chao; Liu, Xiaojun; Wang, Xueding

2016-02-01

Conventional gold standard histopathologic diagnosis requires information of both high resolution structural and chemical changes in tissue. Providing optical information at ultrasonic resolution, photoacoustic (PA) technique could provide highly sensitive and highly accurate tissue characterization noninvasively in the authentic in vivo environment, offering a replacement for histopathology. A two-dimensional (2D) physio-chemical spectrogram (PCS) combining micrometer to centimeter morphology and chemical composition simultaneously can be generated for each biological sample with PA measurements at multiple optical wavelengths. This spectrogram presents a unique 2D “physio-chemical signature” for any specific type of tissue. Comprehensive analysis of PCS, termed PA physio-chemical analysis (PAPCA), can lead to very rich diagnostic information, including the contents of all relevant molecular and chemical components along with their corresponding histological microfeatures, comparable to those accessible by conventional histology. PAPCA could contribute to the diagnosis of many diseases involving diffusive patterns such as fatty liver.

Data collection and simulation of high range resolution laser radar for surface mine detection

NASA Astrophysics Data System (ADS)

Steinvall, Ove; Chevalier, Tomas; Larsson, Håkan

2006-05-01

Rapid and efficient detection of surface mines, IED's (Improvised Explosive Devices) and UXO (Unexploded Ordnance) is of high priority in military conflicts. High range resolution laser radars combined with passive hyper/multispectral sensors offer an interesting concept to help solving this problem. This paper reports on laser radar data collection of various surface mines in different types of terrain. In order to evaluate the capability of 3D imaging for detecting and classifying the objects of interest a scanning laser radar was used to scan mines and surrounding terrain with high angular and range resolution. These data were then fed into a laser radar model capable of generating range waveforms for a variety of system parameters and combinations of different targets and backgrounds. We can thus simulate a potential system by down sampling to relevant pixel sizes and laser/receiver characteristics. Data, simulations and examples will be presented.

Performance study of double SOI image sensors

NASA Astrophysics Data System (ADS)

Miyoshi, T.; Arai, Y.; Fujita, Y.; Hamasaki, R.; Hara, K.; Ikegami, Y.; Kurachi, I.; Nishimura, R.; Ono, S.; Tauchi, K.; Tsuboyama, T.; Yamada, M.

2018-02-01

Double silicon-on-insulator (DSOI) sensors composed of two thin silicon layers and one thick silicon layer have been developed since 2011. The thick substrate consists of high resistivity silicon with p-n junctions while the thin layers are used as SOI-CMOS circuitry and as shielding to reduce the back-gate effect and crosstalk between the sensor and the circuitry. In 2014, a high-resolution integration-type pixel sensor, INTPIX8, was developed based on the DSOI concept. This device is fabricated using a Czochralski p-type (Cz-p) substrate in contrast to a single SOI (SSOI) device having a single thin silicon layer and a Float Zone p-type (FZ-p) substrate. In the present work, X-ray spectra of both DSOI and SSOI sensors were obtained using an Am-241 radiation source at four gain settings. The gain of the DSOI sensor was found to be approximately three times that of the SSOI device because the coupling capacitance is reduced by the DSOI structure. An X-ray imaging demonstration was also performed and high spatial resolution X-ray images were obtained.

Investigation on the separability of slums by multi-aspect TerraSAR-X dual-co-polarized high resolution spotlight images based on the multi-scale evaluation of local distributions

NASA Astrophysics Data System (ADS)

Schmitt, Andreas; Sieg, Tobias; Wurm, Michael; Taubenböck, Hannes

2018-02-01

Following recent advances in distinguishing settlements vs. non-settlement areas from latest SAR data, the question arises whether a further automatic intra-urban delineation and characterization of different structural types is possible. This paper studies the appearance of the structural type ;slums; in high resolution SAR images. Geocoded Kennaugh elements are used as backscatter information and Schmittlet indices as descriptor of local texture. Three cities with a significant share of slums (Cape Town, Manila, Mumbai) are chosen as test sites. These are imaged by TerraSAR-X in the dual-co-polarized high resolution spotlight mode in any available aspect angle. Representative distributions are estimated and fused by a robust approach. Our observations identify a high similarity of slums throughout all three test sites. The derived similarity maps are validated with reference data sets from visual interpretation and ground truth. The final validation strategy is based on completeness and correctness versus other classes in relation to the similarity. High accuracies (up to 87%) in identifying morphologic slums are reached for Cape Town. For Manila (up to 60%) and Mumbai (up to 54%), the distinction is more difficult due to their complex structural configuration. Concluding, high resolution SAR data can be suitable to automatically trace potential locations of slums. Polarimetric information and the incidence angle seem to have a negligible impact on the results whereas the intensity patterns and the passing direction of the satellite are playing a key role. Hence, the combination of intensity images (brightness) acquired from ascending and descending orbits together with Schmittlet indices (spatial pattern) promises best results. The transfer from the automatically recognized physical similarity to the semantic interpretation remains challenging.

Characterizing Arctic Sea Ice Topography Using High-Resolution IceBridge Data

NASA Technical Reports Server (NTRS)

Petty, Alek; Tsamados, Michel; Kurtz, Nathan; Farrell, Sinead; Newman, Thomas; Harbeck, Jeremy; Feltham, Daniel; Richter-Menge, Jackie

2016-01-01

We present an analysis of Arctic sea ice topography using high resolution, three-dimensional, surface elevation data from the Airborne Topographic Mapper, flown as part of NASA's Operation IceBridge mission. Surface features in the sea ice cover are detected using a newly developed surface feature picking algorithm. We derive information regarding the height, volume and geometry of surface features from 2009-2014 within the Beaufort/Chukchi and Central Arctic regions. The results are delineated by ice type to estimate the topographic variability across first-year and multi-year ice regimes.

Self-Powered High-Resolution and Pressure-Sensitive Triboelectric Sensor Matrix for Real-Time Tactile Mapping.

PubMed

Wang, Xiandi; Zhang, Hanlu; Dong, Lin; Han, Xun; Du, Weiming; Zhai, Junyi; Pan, Caofeng; Wang, Zhong Lin

2016-04-20

A triboelectric sensor matrix (TESM) can accurately track and map 2D tactile sensing. A self-powered, high-resolution, pressure-sensitive, flexible and durable TESM with 16 × 16 pixels is fabricated for the fast detection of single-point and multi-point touching. Using cross-locating technology, a cross-type TESM with 32 × 20 pixels is developed for more rapid tactile mapping, which significantly reduces the addressing lines from m × n to m + n. © 2016 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Techniques for automatic large scale change analysis of temporal multispectral imagery

NASA Astrophysics Data System (ADS)

Mercovich, Ryan A.

Change detection in remotely sensed imagery is a multi-faceted problem with a wide variety of desired solutions. Automatic change detection and analysis to assist in the coverage of large areas at high resolution is a popular area of research in the remote sensing community. Beyond basic change detection, the analysis of change is essential to provide results that positively impact an image analyst's job when examining potentially changed areas. Present change detection algorithms are geared toward low resolution imagery, and require analyst input to provide anything more than a simple pixel level map of the magnitude of change that has occurred. One major problem with this approach is that change occurs in such large volume at small spatial scales that a simple change map is no longer useful. This research strives to create an algorithm based on a set of metrics that performs a large area search for change in high resolution multispectral image sequences and utilizes a variety of methods to identify different types of change. Rather than simply mapping the magnitude of any change in the scene, the goal of this research is to create a useful display of the different types of change in the image. The techniques presented in this dissertation are used to interpret large area images and provide useful information to an analyst about small regions that have undergone specific types of change while retaining image context to make further manual interpretation easier. This analyst cueing to reduce information overload in a large area search environment will have an impact in the areas of disaster recovery, search and rescue situations, and land use surveys among others. By utilizing a feature based approach founded on applying existing statistical methods and new and existing topological methods to high resolution temporal multispectral imagery, a novel change detection methodology is produced that can automatically provide useful information about the change occurring in large area and high resolution image sequences. The change detection and analysis algorithm developed could be adapted to many potential image change scenarios to perform automatic large scale analysis of change.

Multi-Resolution Climate Ensemble Parameter Analysis with Nested Parallel Coordinates Plots.

PubMed

Wang, Junpeng; Liu, Xiaotong; Shen, Han-Wei; Lin, Guang

2017-01-01

Due to the uncertain nature of weather prediction, climate simulations are usually performed multiple times with different spatial resolutions. The outputs of simulations are multi-resolution spatial temporal ensembles. Each simulation run uses a unique set of values for multiple convective parameters. Distinct parameter settings from different simulation runs in different resolutions constitute a multi-resolution high-dimensional parameter space. Understanding the correlation between the different convective parameters, and establishing a connection between the parameter settings and the ensemble outputs are crucial to domain scientists. The multi-resolution high-dimensional parameter space, however, presents a unique challenge to the existing correlation visualization techniques. We present Nested Parallel Coordinates Plot (NPCP), a new type of parallel coordinates plots that enables visualization of intra-resolution and inter-resolution parameter correlations. With flexible user control, NPCP integrates superimposition, juxtaposition and explicit encodings in a single view for comparative data visualization and analysis. We develop an integrated visual analytics system to help domain scientists understand the connection between multi-resolution convective parameters and the large spatial temporal ensembles. Our system presents intricate climate ensembles with a comprehensive overview and on-demand geographic details. We demonstrate NPCP, along with the climate ensemble visualization system, based on real-world use-cases from our collaborators in computational and predictive science.

Correlation and agreement between eplet mismatches calculated using serological, low-intermediate and high resolution molecular human leukocyte antigen typing methods.

PubMed

Fidler, Samantha; D'Orsogna, Lloyd; Irish, Ashley B; Lewis, Joshua R; Wong, Germaine; Lim, Wai H

2018-03-02

Structural human leukocyte antigen (HLA) matching at the eplet level can be identified by HLAMatchmaker, which requires the entry of four-digit alleles. The aim of this study was to evaluate the agreement between eplet mismatches calculated by serological and two-digit typing methods compared to high-resolution four-digit typing. In a cohort of 264 donor/recipient pairs, the evaluation of measurement error was assessed using intra-class correlation to confirm the absolute agreement between the number of eplet mismatches at class I (HLA-A, -B, C) and II loci (HLA-DQ and -DR) calculated using serological or two-digit molecular typing compared to four-digit molecular typing methods. The proportion of donor/recipient pairs with a difference of >5 eplet mismatches between the HLA typing methods was also determined. Intra-class correlation coefficients between serological and four-digit molecular typing methods were 0.969 (95% confidence intervals [95% CI] 0.960-0.975) and 0.926 (95% CI 0.899-0.944), respectively; and 0.995 (95% CI 0.994-0.996) and 0.993 (95% CI 0.991-0.995), respectively between two-digit and four-digit molecular typing methods. The proportion of donor/recipient pairs with a difference of >5 eplet mismatches at class I and II loci was 4% and 16% for serological versus four-digit molecular typing methods, and 0% and 2% for two-digit versus four-digit molecular typing methods, respectively. In this small predominantly Caucasian population, compared with serology, there is a high level of agreement in the number of eplet mismatches calculated using two-compared to four-digit molecular HLA-typing methods, suggesting that two-digit typing may be sufficient in determining eplet mismatch load in kidney transplantation.

Amperometric Carbon Fiber Nitrite Microsensor for In Situ Biofilm Monitoring

EPA Science Inventory

A highly selective needle type solid state amperometric nitrite microsensor based on direct nitrite oxidation on carbon fiber was developed using a simplified fabrication method. The microsensor’s tip diameter was approximately 7 µm, providing a high spatial resolution of at lea...

Spatial Modeling of Geometallurgical Properties: Techniques and a Case Study

DOE Office of Scientific and Technical Information (OSTI.GOV)

Deutsch, Jared L., E-mail: jdeutsch@ualberta.ca; Palmer, Kevin; Deutsch, Clayton V.

High-resolution spatial numerical models of metallurgical properties constrained by geological controls and more extensively by measured grade and geomechanical properties constitute an important part of geometallurgy. Geostatistical and other numerical techniques are adapted and developed to construct these high-resolution models accounting for all available data. Important issues that must be addressed include unequal sampling of the metallurgical properties versus grade assays, measurements at different scale, and complex nonlinear averaging of many metallurgical parameters. This paper establishes techniques to address each of these issues with the required implementation details and also demonstrates geometallurgical mineral deposit characterization for a copper–molybdenum deposit inmore » South America. High-resolution models of grades and comminution indices are constructed, checked, and are rigorously validated. The workflow demonstrated in this case study is applicable to many other deposit types.« less

Aircraft Detection in High-Resolution SAR Images Based on a Gradient Textural Saliency Map

PubMed Central

Tan, Yihua; Li, Qingyun; Li, Yansheng; Tian, Jinwen

2015-01-01

This paper proposes a new automatic and adaptive aircraft target detection algorithm in high-resolution synthetic aperture radar (SAR) images of airport. The proposed method is based on gradient textural saliency map under the contextual cues of apron area. Firstly, the candidate regions with the possible existence of airport are detected from the apron area. Secondly, directional local gradient distribution detector is used to obtain a gradient textural saliency map in the favor of the candidate regions. In addition, the final targets will be detected by segmenting the saliency map using CFAR-type algorithm. The real high-resolution airborne SAR image data is used to verify the proposed algorithm. The results demonstrate that this algorithm can detect aircraft targets quickly and accurately, and decrease the false alarm rate. PMID:26378543

High Angular Resolution and Lightweight X-Ray Optics for Astronomical Missions

NASA Technical Reports Server (NTRS)

Zhang, W. W.; Biskach, M. P.; Blake, P. N.; Chan, K. W.; Evans, T. C.; Hong, M.; Jones, W. D.; Jones, W. D.; Kolos, L. D.; Mazzarella, J. M.;

Dual Double-Wedge Pseudo-Depolarizer with Anamorphic PSF

NASA Technical Reports Server (NTRS)

Hill, Peter; Thompson, Patrick

2012-01-01

A polarized scene, which may occur at oblique illumination angles, creates a radiometric signal that varies as a function of viewing angle. One common optical component that is used to minimize such an effect is a polarization scrambler or depolarizer. As part of the CLARREO mission, the SOLARIS instrument project at Goddard Space Flight Center has developed a new class of polarization scramblers using a dual double-wedge pseudo-depolarizer that produces an anamorphic point spread function (PSF). The SOLARIS instrument uses two Wollaston type scramblers in series, each with a distinct wedge angle, to image a pseudo-depolarized scene that is free of eigenstates. Since each wedge is distinct, the scrambler is able to produce an anamorphic PSF that maintains high spatial resolution in one dimension by sacrificing the spatial resolution in the other dimension. This scrambler geometry is ideal for 1-D imagers, such as pushbroom slit spectrometers, which require high spectral resolution, high spatial resolution, and low sensitivity to polarized light. Moreover, the geometry is applicable to a wide range of scientific instruments that require both high SNR (signal-to-noise ratio) and low sensitivity to polarized scenes

Evaluation of high resolution ultrasound as a tool for assessing the 3D volume of blood clots during in vitro thrombolysis.

PubMed

Auboire, Laurent; Escoffre, Jean-Michel; Fouan, Damien; Jacquet, Jean-René; Ossant, Frédéric; Grégoire, Jean-Marc; Bouakaz, Ayache

2017-07-24

Thrombosis is a major cause of several diseases, i.e. myocardial infarction, cerebral stroke and pulmonary embolism. Thrombolytic therapies are required to induce fast and efficient recanalization of occluded vessels. To evaluate the in vitro efficacy of these thrombolytic strategies, measuring clot dissolution is essential. This study aimed to evaluate and validate high resolution ultrasound as a tool to assess the exact volume of clots in 3D and in real time during in vitro thrombolytic drug testing. This new method was validated by measuring the effects of concentration range of recombinant tissue type plasminogen activator on a blood clot during complete occlusion or 70% stenosis of a vessel. This study shows that high resolution ultrasound imaging allows for a real-time assessment of the 3D volume of a blood clot with negligible inter- and intra-operator variabilities. The conclusions drawn from this study demonstrate the promising potential of high resolution ultrasound imaging for the in vitro assessment of new thrombolytic drugs.

Combining one-step Sanger sequencing with phasing probe hybridization for HLA class I typing yields rapid, G-group resolution predicting 99% of unique full length protein sequences.

PubMed

Tu, Bin; Masaberg, Carly; Hou, Lihua; Behm, Daniel; Brescia, Peter; Cha, Nuri; Kariyawasam, Kanthi; Lee, Jar How; Nong, Thoa; Sells, John; Tausch, Paul; Yang, Ruyan; Ng, Jennifer; Hurley, Carolyn Katovich

2017-02-01

Sanger-based DNA sequencing of exons 2+3 of HLA class I alleles from a heterozygote frequently results in two or more alternative genotypes. This study was undertaken to reduce the time and effort required to produce a single high resolution HLA genotype. Samples were typed in parallel by Sanger sequencing and oligonucleotide probe hybridization. This workflow, together with optimization of analysis software, was tested and refined during the typing of over 42,000 volunteers for an unrelated hematopoietic progenitor cell donor registry. Next generation DNA sequencing (NGS) was applied to over 1000 of these samples to identify the alleles present within the G group designations. Single genotypes at G level resolution were obtained for over 95% of the loci without additional assays. The vast majority of alleles identified (>99%) were the primary allele giving the G groups their name. Only 0.7% of the alleles identified encoded protein variants that were not detected by a focus on the antigen recognition domain (ARD)-encoding exons. Our combined method routinely provides biologically relevant typing resolution at the level of the ARD. It can be applied to both single samples or to large volume typing supporting either bone marrow or solid organ transplantation using technologies currently available in many HLA laboratories. © 2017 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.

Magnified hard x-ray microtomography: toward tomography with submicron resolution

NASA Astrophysics Data System (ADS)

Schroer, Christian G.; Benner, Boris; Guenzler, Til F.; Kuhlmann, Marion; Lengeler, Bruno; Rau, Christoph; Weitkamp, Timm; Snigirev, Anatoly A.; Snigireva, Irina

2002-01-01

Parabolic compound refractive lenses (PCRLs) are high quality imaging optics for hard x-rays that can be used as an objective lens in a new type of hard x-ray full field microscope. Using an aluminium PCRL, this new type of microscope has been shown to have a resolution of 350 nm. Further improvement of the resolution down to 50 nm can be expected using beryllium as a lens material. The large depth of field (several mm) of the microscope results in sharp projection images for samples that fit into the field of view of about 300 micrometers. This allows to combine magnified imaging with tomographic techniques. First results of magnified microtomography are shown. Contrast formation in the microscope and the consequences for tomographic reconstruction are discussed. An outlook on further developments is given.

Bioinspired enantioselective synthesis of crinine-type alkaloids via iridium-catalyzed asymmetric hydrogenation of enones† †Electronic supplementary information (ESI) available. See DOI: 10.1039/c7sc02112g Click here for additional data file.

PubMed Central

Zuo, Xiao-Dong; Guo, Shu-Min; Yang, Rui

2017-01-01

A bioinspired enantioselective synthesis of crinine-type alkaloids has been developed by iridium-catalyzed asymmetric hydrogenation of racemic cycloenones. The method features a biomimetic stereodivergent resolution of the substrates bearing a remote arylated quaternary stereocenter. Using this protocol, 24 crinine-type alkaloids and 8 analogues were synthesized in a concise and rapid way with high yield and high enantioselectivity. PMID:28989653

Wave Phenomena Associated with Interplanetary Shocks

NASA Astrophysics Data System (ADS)

Golla, T.; MacDowall, R. J.

2016-12-01

Although laboratory and space-based experiments were used for the last several decades to study the collisionless shocks, several questions remain less than fully understood. These include: (1) what type of wave-particle energy dissipation is responsible for the shock formation, (2) what type of in-situ waves occur in the upstream, transition and downstream regions, and (3) which physical processes are responsible for the excitation of the fundamental and second harmonic solar type II radio emissions. In this study, we will address these issues using (1) the in situ and radio wave data obtained by the WAVES experiments of the STEREO A and B, and WIND spacecraft, especially the high time resolution data from the time domain samplers (TDS) of these WAVES experiments and (2) the Fourier, wavelet and higher order spectral analysis techniques. Using the in situ wave data, especially the high time resolution data observed during the local type II bursts, we will identify the nonlinear processes associated with these solar radio emissions. Comparing the estimated radio intensities by the known emission mechanisms for the observed peak Langmuir wave intensities with the observed peak radio intensities of type II bursts, we will identify the emission mechanisms.

Television experiment for Mariner Mars 1971

USGS Publications Warehouse

Masursky, H.; Batson, R.; Borgeson, W.; Carr, M.; McCauley, J.; Milton, D.; Wildey, R.; Wilhelms, D.; Murray, B.; Horowitz, N.; Leighton, R.; Sharp, R.; Thompson, W.; Briggs, G.; Chandeysson, P.; Shipley, E.; Sagan, C.; Pollack, J.; Lederberg, J.; Levinthal, E.; Hartmann, W.; McCord, T.; Smith, B.; Davies, M.; De Vaucouleurs, G.; Leovy, C.

1970-01-01

The Television Experiment objectives are to provide imaging data which will complement previously gathered data and extend our knowledge of Mars. The two types of investigations will be fixed-feature (for mapping) and variable-feature (for surface and atmospheric changes). Two cameras with a factor-of-ten difference in resolution will be used on each spacecraft for medium- and high-resolution imagery. Mapping of 70% of the planet's surface will be provided by medium-resolution imagery. Spot coverage of about 5% of the surface will be possible with the high-resolution imagery. The experiment's 5 Principal Investigators and 21 Co-Investigators are organized into a team. Scientific disciplines and technical task groups have been formed to provide the formulation of experiment requirements for mission planning and instrument development. It is expected that the team concept will continue through the operational and reporting phases of the Mariner Mars 1971 Project. ?? 1970.

High Resolution Melting (HRM) applied to wine authenticity.

PubMed

Pereira, Leonor; Gomes, Sónia; Castro, Cláudia; Eiras-Dias, José Eduardo; Brazão, João; Graça, António; Fernandes, José R; Martins-Lopes, Paula

2017-02-01

Wine authenticity methods are in increasing demand mainly in Denomination of Origin designations. The DNA-based methodologies are a reliable means of tracking food/wine varietal composition. The main aim of this work was the study of High Resolution Melting (HRM) application as a screening method for must and wine authenticity. Three sample types (leaf, must and wine) were used to validate the three developed HRM assays (Vv1-705bp; Vv2-375bp; and Vv3-119bp). The Vv1 HRM assay was only successful when applied to leaf and must samples. The Vv2 HRM assay successfully amplified all sample types, allowing genotype discrimination based on melting temperature values. The smallest amplicon, Vv3, produced a coincident melting curve shape in all sample types (leaf and wine) with corresponding genotypes. This study presents sensitive, rapid and efficient HRM assays applied for the first time to wine samples suitable for wine authenticity purposes. Copyright © 2016 Elsevier Ltd. All rights reserved.

EXPLORING DATA-DRIVEN SPECTRAL MODELS FOR APOGEE M DWARFS

NASA Astrophysics Data System (ADS)

Lua Birky, Jessica; Hogg, David; Burgasser, Adam J.; Jessica Birky

2018-01-01

The Cannon (Ness et al. 2015; Casey et al. 2016) is a flexible, data-driven spectral modeling and parameter inference framework, demonstrated on high-resolution Apache Point Galactic Evolution Experiment (APOGEE; λ/Δλ~22,500, 1.5-1.7µm) spectra of giant stars to estimate stellar labels (Teff, logg, [Fe/H], and chemical abundances) to precisions higher than the model-grid pipeline. The lack of reliable stellar parameters reported by the APOGEE pipeline for temperatures less than ~3550K, motivates extension of this approach to M dwarf stars. Using a training set of 51 M dwarfs with spectral types ranging M0-M9 obtained from SDSS optical spectra, we demonstrate that the Cannon can infer spectral types to a precision of +/-0.6 types, making it an effective tool for classifying high-resolution near-infrared spectra. We discuss the potential for extending this work to determine the physical stellar labels Teff, logg, and [Fe/H].This work is supported by the SDSS Faculty and Student (FAST) initiative.

Super-Resolution Person Re-Identification With Semi-Coupled Low-Rank Discriminant Dictionary Learning.

PubMed

Jing, Xiao-Yuan; Zhu, Xiaoke; Wu, Fei; Hu, Ruimin; You, Xinge; Wang, Yunhong; Feng, Hui; Yang, Jing-Yu

2017-03-01

Person re-identification has been widely studied due to its importance in surveillance and forensics applications. In practice, gallery images are high resolution (HR), while probe images are usually low resolution (LR) in the identification scenarios with large variation of illumination, weather, or quality of cameras. Person re-identification in this kind of scenarios, which we call super-resolution (SR) person re-identification, has not been well studied. In this paper, we propose a semi-coupled low-rank discriminant dictionary learning (SLD 2 L) approach for SR person re-identification task. With the HR and LR dictionary pair and mapping matrices learned from the features of HR and LR training images, SLD 2 L can convert the features of the LR probe images into HR features. To ensure that the converted features have favorable discriminative capability and the learned dictionaries can well characterize intrinsic feature spaces of the HR and LR images, we design a discriminant term and a low-rank regularization term for SLD 2 L. Moreover, considering that low resolution results in different degrees of loss for different types of visual appearance features, we propose a multi-view SLD 2 L (MVSLD 2 L) approach, which can learn the type-specific dictionary pair and mappings for each type of feature. Experimental results on multiple publicly available data sets demonstrate the effectiveness of our proposed approaches for the SR person re-identification task.

Characterization of primary organic aerosol emissions from meat cooking, trash burning, and motor vehicles with high-resolution aerosol mass spectrometry and comparison with ambient and chamber observations.

PubMed

Mohr, Claudia; Huffman, Alex; Cubison, Michael J; Aiken, Allison C; Docherty, Kenneth S; Kimmel, Joel R; Ulbrich, Ingrid M; Hannigan, Michael; Jimenez, Jose L

2009-04-01

Organic aerosol (OA) emissions from motor vehicles, meat-cooking and trash burning are analyzed here using a high-resolution aerosol mass spectrometer (AMS). High resolution data show that aerosols emitted by combustion engines and plastic burning are dominated by hydrocarbon-like organic compounds. Meat cooking and especially paper burning emissions contain significant fractions of oxygenated organic compounds; however, their unit-resolution mass spectral signatures are very similar to those from ambient hydrocarbon-like OA, and very different from the mass spectra of ambient secondary or oxygenated OA (OOA). Thus, primary OA from these sources is unlikelyto be a significant direct source of ambient OOA. There are significant differences in high-resolution tracer m/zs that may be useful for differentiating some of these sources. Unlike in most ambient spectra, all of these sources have low total m/z 44 and this signal is not dominated by the CO2+ ion. All sources have high m/z 57, which is low during high OOA ambient periods. Spectra from paper burning are similar to some types of biomass burning OA, with elevated m/z 60. Meat cooking aerosols also have slightly elevated m/z 60, whereas motor vehicle emissions have very low signal at this m/z.

Development of a Cost-Effective Modular Pixelated NaI(Tl) Detector for Clinical SPECT Applications

PubMed Central

Rozler, Mike; Liang, Haoning; Chang, Wei

2013-01-01

A new pixelated detector for high-resolution clinical SPECT applications was designed and tested. The modular detector is based on a scintillator block comprised of 2.75×2.75×10 mm3 NaI(Tl) pixels and decoded by an array of 51 mm diameter single-anode PMTs. Several configurations, utilizing two types of PMTs, were evaluated using a collimated beam source to measure positioning accuracy directly. Good pixel separation was observed, with correct pixel identification ranging from 60 to 72% averaged over the entire area of the modules, depending on the PMT type and configuration. This translates to a significant improvement in positioning accuracy compared to continuous slab detectors of the same thickness, along with effective reduction of “dead” space at the edges. The observed 10% average energy resolution compares well to continuous slab detectors. The combined performance demonstrates the suitability of pixelated detectors decoded with a relatively small number of medium-sized PMTs as a cost-effective approach for high resolution clinical SPECT applications, in particular those involving curved detector geometries. PMID:24146436

Transmission type flat-panel X-ray source using ZnO nanowire field emitters

DOE Office of Scientific and Technical Information (OSTI.GOV)

Chen, Daokun; Song, Xiaomeng; Zhang, Zhipeng

2015-12-14

A transmission type flat-panel X-ray source in diode structure was fabricated. Large-scale patterned ZnO nanowires grown on a glass substrate by thermal oxidation were utilized as field emitters, and tungsten thin film coated on silica glass was used as the transmission anode. Uniform distribution of X-ray generation was achieved, which benefited from the uniform electron emission from ZnO nanowires. Self-ballasting effect induced by the intrinsic resistance of ZnO nanowire and decreasing of screening effect caused by patterned emitters account for the uniform emission. Characteristic X-ray peaks of W-L lines and bremsstrahlung X-rays have been observed under anode voltages at amore » range of 18–20 kV, the latter of which were the dominant X-ray signals. High-resolution X-ray images with spatial resolution less than 25 μm were obtained by the flat-panel X-ray source. The high resolution was attributed to the small divergence angle of the emitted X-rays from the transmission X-ray source.« less

Multiresolution quantification of deciduousness in West-Central African forests

NASA Astrophysics Data System (ADS)

Viennois, G.; Barbier, N.; Fabre, I.; Couteron, P.

2013-11-01

The characterization of leaf phenology in tropical forests is of major importance for forest typology as well as to improve our understanding of earth-atmosphere-climate interactions or biogeochemical cycles. The availability of satellite optical data with a high temporal resolution has permitted the identification of unexpected phenological cycles, particularly over the Amazon region. A primary issue in these studies is the relationship between the optical reflectance of pixels of 1 km or more in size and ground information of limited spatial extent. In this paper, we demonstrate that optical data with high to very-high spatial resolution can help bridge this scale gap by providing snapshots of the canopy that allow discernment of the leaf-phenological stage of trees and the proportions of leaved crowns within the canopy. We also propose applications for broad-scale forest characterization and mapping in West-Central Africa over an area of 141 000 km2. Eleven years of the Moderate Resolution Imaging Spectroradiometer (MODIS) Enhanced Vegetation Index (EVI) data were averaged over the wet and dry seasons to provide a data set of optimal radiometric quality at a spatial resolution of 250 m. Sample areas covered at a very-high (GeoEye) and high (SPOT-5) spatial resolution were used to identify forest types and to quantify the proportion of leaved trees in the canopy. The dry-season EVI was positively correlated with the proportion of leaved trees in the canopy. This relationship allowed the conversion of EVI into canopy deciduousness at the regional level. On this basis, ecologically important forest types could be mapped, including young secondary, open Marantaceae, Gilbertiodendron dewevrei and swamp forests. We show that in West-Central African forests, a large share of the variability in canopy reflectance, as captured by the EVI, is due to variation in the proportion of leaved trees in the upper canopy, thereby opening new perspectives for biodiversity and carbon-cycle applications.

Multiresolution quantification of deciduousness in West Central African forests

NASA Astrophysics Data System (ADS)

Viennois, G.; Barbier, N.; Fabre, I.; Couteron, P.

2013-04-01

The characterization of leaf phenology in tropical forests is of major importance and improves our understanding of earth-atmosphere-climate interactions. The availability of satellite optical data with a high temporal resolution has permitted the identification of unexpected phenological cycles, particularly over the Amazon region. A primary issue in these studies is the relationship between the optical reflectance of pixels of 1 km or more in size and ground information of limited spatial extent. In this paper, we demonstrate that optical data with high to very-high spatial resolution can help bridge this scale gap by providing snapshots of the canopy that allow discernment of the leaf-phenological stage of trees and the proportions of leaved crowns within the canopy. We also propose applications for broad-scale forest characterization and mapping in West Central Africa over an area of 141 000 km2. Eleven years of the Moderate Resolution Imaging Spectroradiometer (MODIS) Enhanced Vegetation Index (EVI) data were averaged over the wet and dry seasons to provide a dataset of optimal radiometric quality at a spatial resolution of 250 m. Sample areas covered at a very-high (GeoEye) and high (SPOT-5) spatial resolution were used to identify forest types and to quantify the proportion of leaved trees in the canopy. The dry season EVI was positively correlated with the proportion of leaved trees in the canopy. This relationship allowed the conversion of EVI into canopy deciduousness at the regional level. On this basis, ecologically important forest types could be mapped, including young secondary, open Marantaceae, Gilbertiodendron dewevrei and swamp forests. We show that in west central African forests, a large share of the variability in canopy reflectance, as captured by the EVI, is due to variation in the proportion of leaved trees in the upper canopy, thereby opening new perspectives for biodiversity and carbon-cycle applications.

Improving Numerical Weather Predictions of Summertime Precipitation Over the Southeastern U.S. Through a High-Resolution Initialization of the Surface State

NASA Technical Reports Server (NTRS)

Case, Jonathan L.; Kumar, Sujay V.; Krikishen, Jayanthi; Jedlovec, Gary J.

2011-01-01

It is hypothesized that high-resolution, accurate representations of surface properties such as soil moisture and sea surface temperature are necessary to improve simulations of summertime pulse-type convective precipitation in high resolution models. This paper presents model verification results of a case study period from June-August 2008 over the Southeastern U.S. using the Weather Research and Forecasting numerical weather prediction model. Experimental simulations initialized with high-resolution land surface fields from the NASA Land Information System (LIS) and sea surface temperature (SST) derived from the Moderate Resolution Imaging Spectroradiometer (MODIS) are compared to a set of control simulations initialized with interpolated fields from the National Centers for Environmental Prediction 12-km North American Mesoscale model. The LIS land surface and MODIS SSTs provide a more detailed surface initialization at a resolution comparable to the 4-km model grid spacing. Soil moisture from the LIS spin-up run is shown to respond better to the extreme rainfall of Tropical Storm Fay in August 2008 over the Florida peninsula. The LIS has slightly lower errors and higher anomaly correlations in the top soil layer, but exhibits a stronger dry bias in the root zone. The model sensitivity to the alternative surface initial conditions is examined for a sample case, showing that the LIS/MODIS data substantially impact surface and boundary layer properties.

Some lessons and thoughts from development of an old-fashioned high-resolution atmospheric general circulation model

NASA Astrophysics Data System (ADS)

Ohfuchi, Wataru; Enomoto, Takeshi; Yoshioka, Mayumi K.; Takaya, Koutarou

2014-05-01

Some high-resolution simulations with a conventional atmospheric general circulation model (AGCM) were conducted right after the first Earth Simulator started operating in the spring of 2002. More simulations with various resolutions followed. The AGCM in this study, AFES (Agcm For the Earth Simulator), is a primitive equation spectral transform method model with a cumulus convection parameterization. In this presentation, some findings from comparisons between high and low-resolution simulations, and some future perspectives of old-fashioned AGCMs will be discussed. One obvious advantage of increasing resolution is capability of resolving the fine structures of topography and atmospheric flow. By increasing resolution from T39 (about 320 km horizontal grid interval) to T79 (160 km), to T159 (80 km) to T319 (40 km), topographic precipitation over Japan becomes increasingly realistic. This feature is necessary for climate and weather studies involving both global and local aspects. In order to resolve submesoscale (about 100 km horizontal scale) atmospheric circulation, about 10-km grid interval is necessary. Comparing T1279 (10 km) simulations with T319 ones, it is found that, for example, the intensity of heavy rain associated with Baiu front and the central pressure of typhoon become more realistic. These realistic submesoscale phenomena should have impact on larger-sale flow through dynamics and thermodynamics. An interesting finding by increasing horizontal resolution of a conventional AGCM is that some cumulus convection parameterizations, such as Arakawa-Schubert type scheme, gradually stop producing precipitation, while some others, such as Emanuel type, do not. With the former, the grid condensation increases with the model resolution to compensate. Which characteristics are more desirable is arguable but it is an important feature one has to consider when developing a high-resolution conventional AGCM. Many may think that conventional primitive equation spectral transform AGCMs, such as AFES, have no future. Developing globally homogeneous nonhydrostatic cloud resolving grid AGCMs is obviously a straightforward direction for the future. However these models will be very expensive for many users for a while, perhaps for the next some decades. On the other hand, old-fashioned AGCMs with a grid interval of 20-100 km will remain to be accurate and efficient tools for many users for many years to come. Also by coupling with a fine-resolution regional nonhydrostatic model, a conventional AGCM may overcome its limitation for use in climate and weather studies in the future.

Principle and design of small-sized and high-definition x-ray machine

NASA Astrophysics Data System (ADS)

Zhao, Anqing

2010-10-01

The paper discusses the circuit design and working principles of VMOS PWM type 75KV10mA high frequency X-ray machine. The system mainly consists of silicon controlled rectifier, VMOS tube PWM type high-frequency and highvoltage inverter circuit, filament inverter circuit, high-voltage rectifier filter circuit and as X-ray tube. The working process can be carried out under the control of a single-chip microcomputer. Due to the small size and high resolution in imaging, the X-ray machine is mostly adopted for emergent medical diagnosis and specific circumstances where nondestructive tests are conducted.

Resolution Enhancement of MODIS-derived Water Indices for Studying Persistent Flooding

NASA Astrophysics Data System (ADS)

Underwood, L. W.; Kalcic, M. T.; Fletcher, R. M.

2012-12-01

Monitoring coastal marshes for persistent flooding and salinity stress is a high priority issue in Louisiana. Remote sensing can identify environmental variables that can be indicators of marsh habitat conditions, and offer timely and relatively accurate information for aiding wetland vegetation management. Monitoring activity accuracy is often limited by mixed pixels which occur when areas represented by the pixel encompasses more than one cover type. Mixtures of marsh grasses and open water in 250m Moderate Resolution Imaging Spectroradiometer (MODIS) data can impede flood area estimation. Flood mapping of such mixtures requires finer spatial resolution data to better represent the cover type composition within 250m MODIS pixel. Fusion of MODIS and Landsat can improve both spectral and temporal resolution of time series products to resolve rapid changes from forcing mechanisms like hurricane winds and storm surge. For this study, using a method for estimating sub-pixel values from a MODIS time series of a Normalized Difference Water Index (NDWI), using temporal weighting, was implemented to map persistent flooding in Louisiana coastal marshes. Ordinarily NDWI computed from daily 250m MODIS pixels represents a mixture of fragmented marshes and water. Here, sub-pixel NDWI values were derived for MODIS data using Landsat 30-m data. Each MODIS pixel was disaggregated into a mixture of the eight cover types according to the classified image pixels falling inside the MODIS pixel. The Landsat pixel means for each cover type inside a MODIS pixel were computed for the Landsat data preceding the MODIS image in time and for the Landsat data succeeding the MODIS image. The Landsat data were then weighted exponentially according to closeness in date to the MODIS data. The reconstructed MODIS data were produced by summing the product of fractional cover type with estimated NDWI values within each cover type. A new daily time series was produced using both the reconstructed 250-m MODIS, with enhanced features, and the approximated daily 30-m high-resolution image based on Landsat data. The algorithm was developed and tested over the Calcasieu-Sabine Basin, which was heavily inundated by storm surge from Hurricane Ike to study the extent and duration of flooding following the storm. Time series for 2000-2009, covering flooding events by Hurricane Rita in 2005 and Hurricane Ike in 2008, were derived. High resolution images were formed for all days in 2008 between the first cloud free Landsat scene and the last cloud-free Landsat scene. To refine and validate flooding maps, each time series was compared to Louisiana Coastwide Reference Monitoring System (CRMS) station water levels adjusted to marsh to optimize thresholds for MODIS-derived time series of NDWI. Seasonal fluctuations were adjusted by subtracting ten year average NDWI for marshes, excluding the hurricane events. Results from different NDWI indices and a combination of indices were compared. Flooding persistence that was mapped with higher-resolution data showed some improvement over the original MODIS time series estimates. The advantage of this novel technique is that improved mapping of extent and duration of inundation can be provided.

Resolution Enhancement of MODIS-Derived Water Indices for Studying Persistent Flooding

NASA Technical Reports Server (NTRS)

Underwood, L. W.; Kalcic, Maria; Fletcher, Rose

2012-01-01

Monitoring coastal marshes for persistent flooding and salinity stress is a high priority issue in Louisiana. Remote sensing can identify environmental variables that can be indicators of marsh habitat conditions, and offer timely and relatively accurate information for aiding wetland vegetation management. Monitoring activity accuracy is often limited by mixed pixels which occur when areas represented by the pixel encompasses more than one cover type. Mixtures of marsh grasses and open water in 250m Moderate Resolution Imaging Spectroradiometer (MODIS) data can impede flood area estimation. Flood mapping of such mixtures requires finer spatial resolution data to better represent the cover type composition within 250m MODIS pixel. Fusion of MODIS and Landsat can improve both spectral and temporal resolution of time series products to resolve rapid changes from forcing mechanisms like hurricane winds and storm surge. For this study, using a method for estimating sub-pixel values from a MODIS time series of a Normalized Difference Water Index (NDWI), using temporal weighting, was implemented to map persistent flooding in Louisiana coastal marshes. Ordinarily NDWI computed from daily 250m MODIS pixels represents a mixture of fragmented marshes and water. Here, sub-pixel NDWI values were derived for MODIS data using Landsat 30-m data. Each MODIS pixel was disaggregated into a mixture of the eight cover types according to the classified image pixels falling inside the MODIS pixel. The Landsat pixel means for each cover type inside a MODIS pixel were computed for the Landsat data preceding the MODIS image in time and for the Landsat data succeeding the MODIS image. The Landsat data were then weighted exponentially according to closeness in date to the MODIS data. The reconstructed MODIS data were produced by summing the product of fractional cover type with estimated NDWI values within each cover type. A new daily time series was produced using both the reconstructed 250-m MODIS, with enhanced features, and the approximated daily 30-m high-resolution image based on Landsat data. The algorithm was developed and tested over the Calcasieu-Sabine Basin, which was heavily inundated by storm surge from Hurricane Ike to study the extent and duration of flooding following the storm. Time series for 2000-2009, covering flooding events by Hurricane Rita in 2005 and Hurricane Ike in 2008, were derived. High resolution images were formed for all days in 2008 between the first cloud free Landsat scene and the last cloud-free Landsat scene. To refine and validate flooding maps, each time series was compared to Louisiana Coastwide Reference Monitoring System (CRMS) station water levels adjusted to marsh to optimize thresholds for MODIS-derived time series of NDWI. Seasonal fluctuations were adjusted by subtracting ten year average NDWI for marshes, excluding the hurricane events. Results from different NDWI indices and a combination of indices were compared. Flooding persistence that was mapped with higher-resolution data showed some improvement over the original MODIS time series estimates. The advantage of this novel technique is that improved mapping of extent and duration of inundation can be provided.

Fabrication of X-ray Microcalorimeter Focal Planes Composed of Two Distinct Pixel Types.

PubMed

Wassell, E J; Adams, J S; Bandler, S R; Betancourt-Martinez, G L; Chiao, M P; Chang, M P; Chervenak, J A; Datesman, A M; Eckart, M E; Ewin, A J; Finkbeiner, F M; Ha, J Y; Kelley, R; Kilbourne, C A; Miniussi, A R; Sakai, K; Porter, F; Sadleir, J E; Smith, S J; Wakeham, N A; Yoon, W

2017-06-01

We are developing superconducting transition-edge sensor (TES) microcalorimeter focal planes for versatility in meeting specifications of X-ray imaging spectrometers including high count-rate, high energy resolution, and large field-of-view. In particular, a focal plane composed of two sub-arrays: one of fine-pitch, high count-rate devices and the other of slower, larger pixels with similar energy resolution, offers promise for the next generation of astrophysics instruments, such as the X-ray Integral Field Unit (X-IFU) instrument on the European Space Agency's Athena mission. We have based the sub-arrays of our current design on successful pixel designs that have been demonstrated separately. Pixels with an all gold X-ray absorber on 50 and 75 micron scales where the Mo/Au TES sits atop a thick metal heatsinking layer have shown high resolution and can accommodate high count-rates. The demonstrated larger pixels use a silicon nitride membrane for thermal isolation, thinner Au and an added bismuth layer in a 250 micron square absorber. To tune the parameters of each sub-array requires merging the fabrication processes of the two detector types. We present the fabrication process for dual production of different X-ray absorbers on the same substrate, thick Au on the small pixels and thinner Au with a Bi capping layer on the larger pixels to tune their heat capacities. The process requires multiple electroplating and etching steps, but the absorbers are defined in a single ion milling step. We demonstrate methods for integrating heatsinking of the two types of pixel into the same focal plane consistent with the requirements for each sub-array, including the limiting of thermal crosstalk. We also discuss fabrication process modifications for tuning the intrinsic transition temperature (T c ) of the bilayers for the different device types through variation of the bilayer thicknesses. The latest results on these "hybrid" arrays will be presented.

Fabrication of X-ray Microcalorimeter Focal Planes Composed of Two Distinct Pixel Types

PubMed Central

Wassell, E. J.; Adams, J. S.; Bandler, S. R.; Betancourt-Martinez, G. L.; Chiao, M. P.; Chang, M. P.; Chervenak, J. A.; Datesman, A. M.; Eckart, M. E.; Ewin, A. J.; Finkbeiner, F. M.; Ha, J. Y.; Kelley, R.; Kilbourne, C. A.; Miniussi, A. R.; Sakai, K.; Porter, F.; Sadleir, J. E.; Smith, S. J.; Wakeham, N. A.; Yoon, W.

2017-01-01

We are developing superconducting transition-edge sensor (TES) microcalorimeter focal planes for versatility in meeting specifications of X-ray imaging spectrometers including high count-rate, high energy resolution, and large field-of-view. In particular, a focal plane composed of two sub-arrays: one of fine-pitch, high count-rate devices and the other of slower, larger pixels with similar energy resolution, offers promise for the next generation of astrophysics instruments, such as the X-ray Integral Field Unit (X-IFU) instrument on the European Space Agency’s Athena mission. We have based the sub-arrays of our current design on successful pixel designs that have been demonstrated separately. Pixels with an all gold X-ray absorber on 50 and 75 micron scales where the Mo/Au TES sits atop a thick metal heatsinking layer have shown high resolution and can accommodate high count-rates. The demonstrated larger pixels use a silicon nitride membrane for thermal isolation, thinner Au and an added bismuth layer in a 250 micron square absorber. To tune the parameters of each sub-array requires merging the fabrication processes of the two detector types. We present the fabrication process for dual production of different X-ray absorbers on the same substrate, thick Au on the small pixels and thinner Au with a Bi capping layer on the larger pixels to tune their heat capacities. The process requires multiple electroplating and etching steps, but the absorbers are defined in a single ion milling step. We demonstrate methods for integrating heatsinking of the two types of pixel into the same focal plane consistent with the requirements for each sub-array, including the limiting of thermal crosstalk. We also discuss fabrication process modifications for tuning the intrinsic transition temperature (Tc) of the bilayers for the different device types through variation of the bilayer thicknesses. The latest results on these “hybrid” arrays will be presented. PMID:28804229

Fabrication of X-ray Microcalorimeter Focal Planes Composed of Two Distinct Pixel Types

NASA Technical Reports Server (NTRS)

Wassell, Edward J.; Adams, Joseph S.; Bandler, Simon R.; Betancour-Martinez, Gabriele L; Chiao, Meng P.; Chang, Meng Ping; Chervenak, James A.; Datesman, Aaron M.; Eckart, Megan E.; Ewin, Audrey J.;

High-Resolution Manometry Evaluation of the Pharynx and Upper Esophageal Sphincter Motility in Patients with Achalasia.

PubMed

Menezes, Mariano A; Herbella, Fernando A M; Patti, Marco G

2015-10-01

The motility of the pharynx and upper esophageal sphincter (UES) is still poorly understood. It is also unclear if the motility of this area may be compromised in patients with achalasia. This study aims to evaluate the motility of the pharynx, UES, and proximal esophagus in patients with esophageal achalasia. Sixty patients with achalasia underwent high-resolution manometry (HRM) (52 % females, mean age 54 years). Esophageal dilatation was classified according to the radiologic diameter in Type I (<4 cm): 6 %; Type II (4-7 cm): 36 %; Type III (7-10 cm): 34 %; and Type IV (>10 cm): 24 %. HRM classified 43 % of the patients as Chicago Type I and 57 % as Type II. Manometric parameters were compared to normal values obtained from a previous study in volunteers. The motility of the velopharynx showed short, premature, and hypertonic contraction. The epiglottis also showed hypertonic contraction. The UES had increased residual pressure. Chicago classification Type II patients had higher UES residual pressure (p = 0.03). The degree of esophageal dilatation did not correlate with manometric parameters. Achalasia may affect the motility of the pharyngo-upper esophageal area. The changes observed may represent functional alterations to prevent aspiration, especially in patients with Chicago classification Type II achalasia.

Application of Curved MPR Algorithm to High Resolution 3 Dimensional T2 Weighted CISS Images for Virtual Uncoiling of Membranous Cochlea as an Aid for Cochlear Morphometry.

PubMed

Kumar, Joish Upendra; Kavitha, Y

2017-02-01

With the use of various surgical techniques, types of implants, the preoperative assessment of cochlear dimensions is becoming increasingly relevant prior to cochlear implantation. High resolution CISS protocol MRI gives a better assessment of membranous cochlea, cochlear nerve, and membranous labyrinth. Curved Multiplanar Reconstruction (MPR) algorithm provides better images that can be used for measuring dimensions of membranous cochlea. To ascertain the value of curved multiplanar reconstruction algorithm in high resolution 3-Dimensional T2 Weighted Gradient Echo Constructive Interference Steady State (3D T2W GRE CISS) imaging for accurate morphometry of membranous cochlea. Fourteen children underwent MRI for inner ear assessment. High resolution 3D T2W GRE CISS sequence was used to obtain images of cochlea. Curved MPR reconstruction algorithm was used to virtually uncoil the membranous cochlea on the volume images and cochlear measurements were done. Virtually uncoiled images of membranous cochlea of appropriate resolution were obtained from the volume data obtained from the high resolution 3D T2W GRE CISS images, after using curved MPR reconstruction algorithm mean membranous cochlear length in the children was 27.52 mm. Maximum apical turn diameter of membranous cochlea was 1.13 mm, mid turn diameter was 1.38 mm, basal turn diameter was 1.81 mm. Curved MPR reconstruction algorithm applied to CISS protocol images facilitates in getting appropriate quality images of membranous cochlea for accurate measurements.

Gridless, pattern-driven point cloud completion and extension

NASA Astrophysics Data System (ADS)

Gravey, Mathieu; Mariethoz, Gregoire

2016-04-01

While satellites offer Earth observation with a wide coverage, other remote sensing techniques such as terrestrial LiDAR can acquire very high-resolution data on an area that is limited in extension and often discontinuous due to shadow effects. Here we propose a numerical approach to merge these two types of information, thereby reconstructing high-resolution data on a continuous large area. It is based on a pattern matching process that completes the areas where only low-resolution data is available, using bootstrapped high-resolution patterns. Currently, the most common approach to pattern matching is to interpolate the point data on a grid. While this approach is computationally efficient, it presents major drawbacks for point clouds processing because a significant part of the information is lost in the point-to-grid resampling, and that a prohibitive amount of memory is needed to store large grids. To address these issues, we propose a gridless method that compares point clouds subsets without the need to use a grid. On-the-fly interpolation involves a heavy computational load, which is met by using a GPU high-optimized implementation and a hierarchical pattern searching strategy. The method is illustrated using data from the Val d'Arolla, Swiss Alps, where high-resolution terrestrial LiDAR data are fused with lower-resolution Landsat and WorldView-3 acquisitions, such that the density of points is homogeneized (data completion) and that it is extend to a larger area (data extension).

Comparing High Definition Live Interactive and Store-and-Forward Consultations to In-Person Examinations.

PubMed

Marchell, Richard; Locatis, Craig; Burges, Gene; Maisiak, Richard; Liu, Wei-Li; Ackerman, Michael

2017-03-01

There is little teledermatology research directly comparing remote methods, even less research with two in-person dermatologist agreement providing a baseline for comparing remote methods, and no research using high definition video as a live interactive method. To compare in-person consultations with store-and-forward and live interactive methods, the latter having two levels of image quality. A controlled study was conducted where patients were examined in-person, by high definition video, and by store-and-forward methods. The order patients experienced methods and residents assigned methods rotated, although an attending always saw patients in-person. The type of high definition video employed, lower resolution compressed or higher resolution uncompressed, was alternated between clinics. Primary and differential diagnoses, biopsy recommendations, and diagnostic and biopsy confidence ratings were recorded. Concordance and confidence were significantly better for in-person versus remote methods and biopsy recommendations were lower. Store-and-forward and higher resolution uncompressed video results were similar and better than those for lower resolution compressed video. Dermatology residents took store-and-forward photos and their quality was likely superior to those normally taken in practice. There were variations in expertise between the attending and second and third year residents. The superiority of in-person consultations suggests the tendencies to order more biopsies or still see patients in-person are often justified in teledermatology and that high resolution uncompressed video can close the resolution gap between store-and-forward and live interactive methods.

Does infantile abduction deficit indicate duane retraction syndrome until disproven?

PubMed

Kim, Jae Hyoung; Hwang, Jeong-Min

2014-11-01

Duane retraction syndrome consists of abduction deficit and palpebral fissure narrowing, upshoots, or downshoots on adduction. Infants with abduction deficit should be considered to have Duane retraction syndrome until disproven, because congenital abducens nerve palsy is extremely rare. The abducens nerve on the affected side is absent in type 1 Duane retraction syndrome and in some type 3 patients. The authors present a 7-month-old girl who showed limitation of abduction simulating Duane retraction syndrome. High-resolution magnetic resonance imaging (MRI) revealed atrophic lateral rectus and present abducens nerve. This report is important because this case showed that congenital abducens nerve palsy exists, although it is extremely rare, and high-resolution MRI could be pivotal for the differentiation of Duane retraction syndrome and congenital abducens nerve palsy in infancy. © The Author(s) 2014.

Spectroscopic Confirmation That 2MASS J07414279–0506464 Is a Mid-type L Dwarf

NASA Astrophysics Data System (ADS)

Cushing, Michael C.; Moskovitz, Nicholas; Gustafsson, Annika

2018-06-01

We present a low-resolution near-infrared spectrum of 2MASS J07414279-0506464, a mid-type L dwarf candidate recently identified by Scholz & Bell. The spectrum was obtained using the Near-Infrared High Throughput Spectrograph (NIHTS) on Lowell Observatory's 4.3 m Discovery Channel Telescope and indicates that 2MASS J07414279-0506464 has a spectral type of L5.

Advances in photographic X-ray imaging for solar astronomy

NASA Technical Reports Server (NTRS)

Moses, J. Daniel; Schueller, R.; Waljeski, K.; Davis, John M.

1989-01-01

The technique of obtaining quantitative data from high resolution soft X-ray photographic images produced by grazing incidence optics was successfully developed to a high degree during the Solar Research Sounding Rocket Program and the S-054 X-Ray Spectrographic Telescope Experiment Program on Skylab. Continued use of soft X-ray photographic imaging in sounding rocket flights of the High Resolution Solar Soft X-Ray Imaging Payload has provided opportunities to further develop these techniques. The developments discussed include: (1) The calibration and use of an inexpensive, commercially available microprocessor controlled drum type film processor for photometric film development; (2) The use of Kodak Technical Pan 2415 film and Kodak SO-253 High Speed Holographic film for improved resolution; and (3) The application of a technique described by Cook, Ewing, and Sutton for determining the film characteristics curves from density histograms of the flight film. Although the superior sensitivity, noise level, and linearity of microchannel plate and CCD detectors attracts the development efforts of many groups working in soft X-ray imaging, the high spatial resolution and dynamic range as well as the reliability and ease of application of photographic media assures the continued use of these techniques in solar X-ray astronomy observations.

Computer-aided diagnosis of lung cancer: definition and detection of ground-glass opacity type of nodules by high-resolution computed tomography.

PubMed

Okada, Tohru; Iwano, Shingo; Ishigaki, Takeo; Kitasaka, Takayuki; Hirano, Yasushi; Mori, Kensaku; Suenaga, Yasuhito; Naganawa, Shinji

2009-02-01

The ground-glass opacity (GGO) of lung cancer is identified only subjectively on computed tomography (CT) images as no quantitative characteristic has been defined for GGOs. We sought to define GGOs quantitatively and to differentiate between GGOs and solid-type lung cancers semiautomatically with a computer-aided diagnosis (CAD). High-resolution CT images of 100 pulmonary nodules (all peripheral lung cancers) were collected from our clinical records. Two radiologists traced the contours of nodules and distinguished GGOs from solid areas. The CT attenuation value of each area was measured. Differentiation between cancer types was assessed by a receiver-operating characteristic (ROC) analysis. The mean CT attenuation of the GGO areas was -618.4 +/- 212.2 HU, whereas that of solid areas was -68.1 +/- 230.3 HU. CAD differentiated between solidand GGO-type lung cancers with a sensitivity of 86.0% and specificity of 96.5% when the threshold value was -370 HU. Four nodules of mixed GGOs were incorrectly classified as the solid type. CAD detected 96.3% of GGO areas when the threshold between GGO and solid areas was 194 HU. Objective definition of GGO area by CT attenuation is feasible. This method is useful for semiautomatic differentiation between GGOs and solid types of lung cancer.

Influence of Gridded Standoff Measurement Resolution on Numerical Bathymetric Inversion

NASA Astrophysics Data System (ADS)

Hesser, T.; Farthing, M. W.; Brodie, K.

2016-02-01

The bathymetry from the surfzone to the shoreline incurs frequent, active movement due to wave energy interacting with the seafloor. Methodologies to measure bathymetry range from point-source in-situ instruments, vessel-mounted single-beam or multi-beam sonar surveys, airborne bathymetric lidar, as well as inversion techniques from standoff measurements of wave processes from video or radar imagery. Each type of measurement has unique sources of error and spatial and temporal resolution and availability. Numerical bathymetry estimation frameworks can use these disparate data types in combination with model-based inversion techniques to produce a "best-estimate of bathymetry" at a given time. Understanding how the sources of error and varying spatial or temporal resolution of each data type affect the end result is critical for determining best practices and in turn increase the accuracy of bathymetry estimation techniques. In this work, we consider an initial step in the development of a complete framework for estimating bathymetry in the nearshore by focusing on gridded standoff measurements and in-situ point observations in model-based inversion at the U.S. Army Corps of Engineers Field Research Facility in Duck, NC. The standoff measurement methods return wave parameters computed using linear wave theory from the direct measurements. These gridded datasets can range in temporal and spatial resolution that do not match the desired model parameters and therefore could lead to a reduction in the accuracy of these methods. Specifically, we investigate the affect of numerical resolution on the accuracy of an Ensemble Kalman Filter bathymetric inversion technique in relation to the spatial and temporal resolution of the gridded standoff measurements. The accuracies of the bathymetric estimates are compared with both high-resolution Real Time Kinematic (RTK) single-beam surveys as well as alternative direct in-situ measurements using sonic altimeters.

Comparative analysis of core genome MLST and SNP typing within a European Salmonella serovar Enteritidis outbreak.

PubMed

Pearce, Madison E; Alikhan, Nabil-Fareed; Dallman, Timothy J; Zhou, Zhemin; Grant, Kathie; Maiden, Martin C J

2018-06-02

Multi-country outbreaks of foodborne bacterial disease present challenges in their detection, tracking, and notification. As food is increasingly distributed across borders, such outbreaks are becoming more common. This increases the need for high-resolution, accessible, and replicable isolate typing schemes. Here we evaluate a core genome multilocus typing (cgMLST) scheme for the high-resolution reproducible typing of Salmonella enterica (S. enterica) isolates, by its application to a large European outbreak of S. enterica serovar Enteritidis. This outbreak had been extensively characterised using single nucleotide polymorphism (SNP)-based approaches. The cgMLST analysis was congruent with the original SNP-based analysis, the epidemiological data, and whole genome MLST (wgMLST) analysis. Combination of the cgMLST and epidemiological data confirmed that the genetic diversity among the isolates predated the outbreak, and was likely present at the infection source. There was consequently no link between country of isolation and genetic diversity, but the cgMLST clusters were congruent with date of isolation. Furthermore, comparison with publicly available Enteritidis isolate data demonstrated that the cgMLST scheme presented is highly scalable, enabling outbreaks to be contextualised within the Salmonella genus. The cgMLST scheme is therefore shown to be a standardised and scalable typing method, which allows Salmonella outbreaks to be analysed and compared across laboratories and jurisdictions. Copyright © 2018. Published by Elsevier B.V.

Competitive amplification of differentially melting amplicons (CADMA) enables sensitive and direct detection of all mutation types by high-resolution melting analysis.

PubMed

Kristensen, Lasse S; Andersen, Gitte B; Hager, Henrik; Hansen, Lise Lotte

2012-01-01

Sensitive and specific mutation detection is of particular importance in cancer diagnostics, prognostics, and individualized patient treatment. However, the majority of molecular methodologies that have been developed with the aim of increasing the sensitivity of mutation testing have drawbacks in terms of specificity, convenience, or costs. Here, we have established a new method, Competitive Amplification of Differentially Melting Amplicons (CADMA), which allows very sensitive and specific detection of all mutation types. The principle of the method is to amplify wild-type and mutated sequences simultaneously using a three-primer system. A mutation-specific primer is designed to introduce melting temperature decreasing mutations in the resulting mutated amplicon, while a second overlapping primer is designed to amplify both wild-type and mutated sequences. When combined with a third common primer very sensitive mutation detection becomes possible, when using high-resolution melting (HRM) as detection platform. The introduction of melting temperature decreasing mutations in the mutated amplicon also allows for further mutation enrichment by fast coamplification at lower denaturation temperature PCR (COLD-PCR). For proof-of-concept, we have designed CADMA assays for clinically relevant BRAF, EGFR, KRAS, and PIK3CA mutations, which are sensitive to, between 0.025% and 0.25%, mutated alleles in a wild-type background. In conclusion, CADMA enables highly sensitive and specific mutation detection by HRM analysis. © 2011 Wiley Periodicals, Inc.

Countercurrent chromatography separation of saponins by skeleton type from Ampelozizyphus amazonicus for off-line ultra-high-performance liquid chromatography/high resolution accurate mass spectrometry analysis and characterisation.

PubMed

de Souza Figueiredo, Fabiana; Celano, Rita; de Sousa Silva, Danila; das Neves Costa, Fernanda; Hewitson, Peter; Ignatova, Svetlana; Piccinelli, Anna Lisa; Rastrelli, Luca; Guimarães Leitão, Suzana; Guimarães Leitão, Gilda

2017-01-20

Ampelozizyphus amazonicus Ducke (Rhamnaceae), a medicinal plant used to prevent malaria, is a climbing shrub, native to the Amazonian region, with jujubogenin glycoside saponins as main compounds. The crude extract of this plant is too complex for any kind of structural identification, and HPLC separation was not sufficient to resolve this issue. Therefore, the aim of this work was to obtain saponin enriched fractions from the bark ethanol extract by countercurrent chromatography (CCC) for further isolation and identification/characterisation of the major saponins by HPLC and MS. The butanol extract was fractionated by CCC with hexane - ethyl acetate - butanol - ethanol - water (1:6:1:1:6; v/v) solvent system yielding 4 group fractions. The collected fractions were analysed by UHPLC-HRMS (ultra-high-performance liquid chromatography/high resolution accurate mass spectrometry) and MS n . Group 1 presented mainly oleane type saponins, and group 3 showed mainly jujubogenin glycosides, keto-dammarane type triterpene saponins and saponins with C 31 skeleton. Thus, CCC separated saponins from the butanol-rich extract by skeleton type. A further purification of group 3 by CCC (ethyl acetate - ethanol - water (1:0.2:1; v/v)) and HPLC-RI was performed in order to obtain these unusual aglycones in pure form. Copyright © 2016 Elsevier B.V. All rights reserved.

Genotyping Applications for Transplantation and Transfusion Management: The Emory Experience.

PubMed

Fasano, Ross M; Sullivan, Harold Cliff; Bray, Robert A; Gebel, Howard M; Meyer, Erin K; Winkler, Annie M; Josephson, Cassandra D; Stowell, Sean R; Sandy Duncan, Alexander; Roback, John D

2017-03-01

Current genotyping methodologies for transplantation and transfusion management employ multiplex systems that allow for simultaneous detection of multiple HLA antigens, human platelet antigens, and red blood cell (RBC) antigens. The development of high-resolution, molecular HLA typing has led to improved outcomes in unrelated hematopoietic stem cell transplants by better identifying compatible alleles of the HLA-A, B, C, DRB1, and DQB1 antigens. In solid organ transplantation, the combination of high-resolution HLA typing with solid-phase antibody identification has proven of value for highly sensitized patients and has significantly reduced incompatible crossmatches at the time of organ allocation. This database-driven, combined HLA antigen/antibody testing has enabled routine implementation of "virtual crossmatching" and may even obviate the need for physical crossmatching. In addition, DNA-based testing for RBC antigens provides an alternative typing method that mitigates many of the limitations of hemagglutination-based phenotyping. Although RBC genotyping has utility in various transfusion settings, it has arguably been most useful for minimizing alloimmunization in the management of transfusion-dependent patients with sickle cell disease or thalassemia. The availability of high-throughput RBC genotyping for both individuals and large populations of donors, along with coordinated informatics systems to compare patients' antigen profiles with available antigen-negative and/or rare blood-typed donors, holds promise for improving the efficiency, reliability, and extent of RBC matching for this population.

New HLA haplotype frequency reference standards: high-resolution and large sample typing of HLA DR-DQ haplotypes in a sample of European Americans.

PubMed

Klitz, W; Maiers, M; Spellman, S; Baxter-Lowe, L A; Schmeckpeper, B; Williams, T M; Fernandez-Viña, M

2003-10-01

A collaborative study involving a large sample of European Americans was typed for the histocompatibility loci of the HLA DR-DQ region and subjected to intensive typing validation measures in order to accurately determine haplotype composition and frequency. The resulting tables have immediate application to HLA typing and allogeneic transplantation. The loci within the DR-DQ region are especially valuable for such an undertaking because of their tight linkage and high linkage disequilibrium. The 3798 haplotypes, derived from 1899 unrelated individuals, had a total of 75 distinct DRB1-DQA1-DQB1 haplotypes. The frequency distribution of the haplotypes was right skewed with haplotypes occurring at a frequency of less than 1% numbering 59 and yet constituting less than 12% of the total sample. Given DRB1 typing, it was possible to infer the exact DQA1 and DQB1 composition of a haplotype with high confidence (>90% likelihood) in 21 of the 35 high-resolution DRB1 alleles present in the sample. Of the DRB1 alleles without high reliability for DQ haplotype inference, only *0401, *0701 and *1302 were common, the remaining 11 DRB1 alleles constituting less than 5% of the total sample. This approach failed for the 13 serologically equivalent DR alleles in which only 33% of DQ haplotypes could be reliably inferred. The 36 DQA1-DQB1 haplotypes present in the total sample conformed to the known pattern of permissible heterodimers. Four DQA1-DQB1 haplotypes, all rare, are reported here for the first time. The haplotype frequency tables are suitable as a reference standard for HLA typing of the DR and DQ loci in European Americans.

A review on high-resolution CMOS delay lines: towards sub-picosecond jitter performance.

PubMed

Abdulrazzaq, Bilal I; Abdul Halin, Izhal; Kawahito, Shoji; Sidek, Roslina M; Shafie, Suhaidi; Yunus, Nurul Amziah Md

2016-01-01

A review on CMOS delay lines with a focus on the most frequently used techniques for high-resolution delay step is presented. The primary types, specifications, delay circuits, and operating principles are presented. The delay circuits reported in this paper are used for delaying digital inputs and clock signals. The most common analog and digitally-controlled delay elements topologies are presented, focusing on the main delay-tuning strategies. IC variables, namely, process, supply voltage, temperature, and noise sources that affect delay resolution through timing jitter are discussed. The design specifications of these delay elements are also discussed and compared for the common delay line circuits. As a result, the main findings of this paper are highlighting and discussing the followings: the most efficient high-resolution delay line techniques, the trade-off challenge found between CMOS delay lines designed using either analog or digitally-controlled delay elements, the trade-off challenge between delay resolution and delay range and the proposed solutions for this challenge, and how CMOS technology scaling can affect the performance of CMOS delay lines. Moreover, the current trends and efforts used in order to generate output delayed signal with low jitter in the sub-picosecond range are presented.

High-resolution ionization detector and array of such detectors

DOEpatents

McGregor, Douglas S [Ypsilanti, MI; Rojeski, Ronald A [Pleasanton, CA

2001-01-16

A high-resolution ionization detector and an array of such detectors are described which utilize a reference pattern of conductive or semiconductive material to form interaction, pervious and measurement regions in an ionization substrate of, for example, CdZnTe material. The ionization detector is a room temperature semiconductor radiation detector. Various geometries of such a detector and an array of such detectors produce room temperature operated gamma ray spectrometers with relatively high resolution. For example, a 1 cm.sup.3 detector is capable of measuring .sup.137 Cs 662 keV gamma rays with room temperature energy resolution approaching 2% at FWHM. Two major types of such detectors include a parallel strip semiconductor Frisch grid detector and the geometrically weighted trapezoid prism semiconductor Frisch grid detector. The geometrically weighted detector records room temperature (24.degree. C.) energy resolutions of 2.68% FWHM for .sup.137 Cs 662 keV gamma rays and 2.45% FWHM for .sup.60 Co 1.332 MeV gamma rays. The detectors perform well without any electronic pulse rejection, correction or compensation techniques. The devices operate at room temperature with simple commercially available NIM bin electronics and do not require special preamplifiers or cooling stages for good spectroscopic results.

Monitoring rangeland dynamics in Senegal with advanced very high resolution radiometer data

USGS Publications Warehouse

Tappan, G. Gray; Tyler, Dean J.; Wehde, M. E.; Moore, Donald G.

1992-01-01

Time‐series Normalized Difference Vegetation Index (NDVI) data, computed from Advanced Very High Resolution Radiometer data, are being used by regional and national programs in the African Sahel to monitor seasonal rangeland conditions. The data are often used as indicators of grazing conditions and drought. However, distinguishing rangelands from other vegetation cover types on NDVI images is difficult. A second complication is that rangeland types and their associated productivity vary geographically by soil type. To effectively assess rangeland conditions, seasonal fluctuations (due to climatic cycles) must be isolated from long‐term production characteristics associated with vegetation type and soil differences. Rangeland NDVI dynamics, including qualitative assessments of rangeland production, and the timing and length of the growing season in Senegal were examined by using 7.4‐km global area coverage satellite data. Analyses were based on 10‐day NDVI composite image data from 1982 through 1989. The NDVI image data were stratified by rangeland and soil polygons derived from locally available resource maps. Time‐series NDVI statistics were calculated from the resource polygons that had been interpreted into high, medium, and low production rangelands. Analysts monitoring rangeland conditions can better identify seasonal anomalies such as drought by comparing production potential within homogeneous; resource polygons with the current NDVI data.

Re-assessing Present Day Global Mass Transport and Glacial Isostatic Adjustment From a Data Driven Approach

NASA Astrophysics Data System (ADS)

Wu, X.; Jiang, Y.; Simonsen, S.; van den Broeke, M. R.; Ligtenberg, S.; Kuipers Munneke, P.; van der Wal, W.; Vermeersen, B. L. A.

2017-12-01

Determining present-day mass transport (PDMT) is complicated by the fact that most observations contain signals from both present day ice melting and Glacial Isostatic Adjustment (GIA). Despite decades of progress in geodynamic modeling and new observations, significant uncertainties remain in both. The key to separate present-day ice mass change and signals from GIA is to include data of different physical characteristics. We designed an approach to separate PDMT and GIA signatures by estimating them simultaneously using globally distributed interdisciplinary data with distinct physical information and a dynamically constructed a priori GIA model. We conducted a high-resolution global reappraisal of present-day ice mass balance with focus on Earth's polar regions and its contribution to global sea-level rise using a combination of ICESat, GRACE gravity, surface geodetic velocity data, and an ocean bottom pressure model. Adding ice altimetry supplies critically needed dual data types over the interiors of ice covered regions to enhance separation of PDMT and GIA signatures, and achieve half an order of magnitude expected higher accuracies for GIA and consequently ice mass balance estimates. The global data based approach can adequately address issues of PDMT and GIA induced geocenter motion and long-wavelength signatures important for large areas such as Antarctica and global mean sea level. In conjunction with the dense altimetry data, we solved for PDMT coefficients up to degree and order 180 by using a higher-resolution GRACE data set, and a high-resolution a priori PDMT model that includes detailed geographic boundaries. The high-resolution approach solves the problem of multiple resolutions in various data types, greatly reduces aliased errors from a low-degree truncation, and at the same time, enhances separation of signatures from adjacent regions such as Greenland and Canadian Arctic territories.

Three-dimensional imaging of micro-specimen by optical scanning holography

NASA Astrophysics Data System (ADS)

Liu, Jung-Ping; Tsou, Cheng-Hao

2017-04-01

Optical scanning holography (OSH) is a scanning-type digital holographic technique. In OSH, a heterodyne interference pattern is generated to raster scan the object. OSH can be operated in the incoherent mode and thus is able to record a fluorescence hologram. In addition, resolution of the OSH is proportional to the density of the interference pattern. Here we use a high-NA microscope objective to generate a dynamic Fresnel zone plate to record a hologram of micro-specimen. The achieved transverse resolution and longitudinal resolution are 0.78μm and 3.1μm, respectively.

Sensitivity and accuracy of high-throughput metabarcoding methods used to describe aquatic communities for early detection of invasve fish species

EPA Science Inventory

For early detection biomonitoring of aquatic invasive species, sensitivity to rare individuals and accurate, high-resolution taxonomic classification are critical to minimize Type I and II detection errors. Given the great expense and effort associated with morphological identifi...

High-Resolution Audio with Inaudible High-Frequency Components Induces a Relaxed Attentional State without Conscious Awareness.

PubMed

Kuribayashi, Ryuma; Nittono, Hiroshi

2017-01-01

High-resolution audio has a higher sampling frequency and a greater bit depth than conventional low-resolution audio such as compact disks. The higher sampling frequency enables inaudible sound components (above 20 kHz) that are cut off in low-resolution audio to be reproduced. Previous studies of high-resolution audio have mainly focused on the effect of such high-frequency components. It is known that alpha-band power in a human electroencephalogram (EEG) is larger when the inaudible high-frequency components are present than when they are absent. Traditionally, alpha-band EEG activity has been associated with arousal level. However, no previous studies have explored whether sound sources with high-frequency components affect the arousal level of listeners. The present study examined this possibility by having 22 participants listen to two types of a 400-s musical excerpt of French Suite No. 5 by J. S. Bach (on cembalo, 24-bit quantization, 192 kHz A/D sampling), with or without inaudible high-frequency components, while performing a visual vigilance task. High-alpha (10.5-13 Hz) and low-beta (13-20 Hz) EEG powers were larger for the excerpt with high-frequency components than for the excerpt without them. Reaction times and error rates did not change during the task and were not different between the excerpts. The amplitude of the P3 component elicited by target stimuli in the vigilance task increased in the second half of the listening period for the excerpt with high-frequency components, whereas no such P3 amplitude change was observed for the other excerpt without them. The participants did not distinguish between these excerpts in terms of sound quality. Only a subjective rating of inactive pleasantness after listening was higher for the excerpt with high-frequency components than for the other excerpt. The present study shows that high-resolution audio that retains high-frequency components has an advantage over similar and indistinguishable digital sound sources in which such components are artificially cut off, suggesting that high-resolution audio with inaudible high-frequency components induces a relaxed attentional state without conscious awareness.

Measuring Large-Scale Social Networks with High Resolution

PubMed Central

Stopczynski, Arkadiusz; Sekara, Vedran; Sapiezynski, Piotr; Cuttone, Andrea; Madsen, Mette My; Larsen, Jakob Eg; Lehmann, Sune

2014-01-01

This paper describes the deployment of a large-scale study designed to measure human interactions across a variety of communication channels, with high temporal resolution and spanning multiple years—the Copenhagen Networks Study. Specifically, we collect data on face-to-face interactions, telecommunication, social networks, location, and background information (personality, demographics, health, politics) for a densely connected population of 1 000 individuals, using state-of-the-art smartphones as social sensors. Here we provide an overview of the related work and describe the motivation and research agenda driving the study. Additionally, the paper details the data-types measured, and the technical infrastructure in terms of both backend and phone software, as well as an outline of the deployment procedures. We document the participant privacy procedures and their underlying principles. The paper is concluded with early results from data analysis, illustrating the importance of multi-channel high-resolution approach to data collection. PMID:24770359

High-spatial-resolution K-band Imaging of Select K2 Campaign Fields

NASA Astrophysics Data System (ADS)

Colón, Knicole D.; Howell, Steve B.; Ciardi, David R.; Barclay, Thomas

2017-12-01

NASA's K2 mission began observing fields along the ecliptic plane in 2014. Each observing campaign lasts approximately 80 days, during which high-precision optical photometry of select astrophysical targets is collected by the Kepler spacecraft. Due to the 4 arcsec pixel scale of the Kepler photometer, significant blending between the observed targets can occur (especially in dense fields close to the Galactic plane). We undertook a program to use the Wide Field Camera (WFCAM) on the 3.8 m United Kingdom InfraRed Telescope (UKIRT) to collect high-spatial-resolution near-infrared images of targets in select K2 campaign fields, which we report here. These 0.4 arcsec resolution K-band images offer the opportunity to perform a variety of science, including vetting exoplanet candidates by identifying nearby stars blended with the target star and estimating the size, color, and type of galaxies observed by K2.

Nanoscale deformation analysis with high-resolution transmission electron microscopy and digital image correlation

DOE PAGES

Wang, Xueju; Pan, Zhipeng; Fan, Feifei; ...

2015-09-10

We present an application of the digital image correlation (DIC) method to high-resolution transmission electron microscopy (HRTEM) images for nanoscale deformation analysis. The combination of DIC and HRTEM offers both the ultrahigh spatial resolution and high displacement detection sensitivity that are not possible with other microscope-based DIC techniques. We demonstrate the accuracy and utility of the HRTEM-DIC technique through displacement and strain analysis on amorphous silicon. Two types of error sources resulting from the transmission electron microscopy (TEM) image noise and electromagnetic-lens distortions are quantitatively investigated via rigid-body translation experiments. The local and global DIC approaches are applied for themore » analysis of diffusion- and reaction-induced deformation fields in electrochemically lithiated amorphous silicon. As a result, the DIC technique coupled with HRTEM provides a new avenue for the deformation analysis of materials at the nanometer length scales.« less

Hyperspectral Remote Sensing of Atmospheric Profiles from Satellites and Aircraft

NASA Technical Reports Server (NTRS)

Smith, W. L.; Zhou, D. K.; Harrison, F. W.; Revercomb, H. E.; Larar, A. M.; Huang, H. L.; Huang, B.

2001-01-01

A future hyperspectral resolution remote imaging and sounding system, called the GIFTS (Geostationary Imaging Fourier Transform Spectrometer), is described. An airborne system, which produces the type of hyperspectral resolution sounding data to be achieved with the GIFTS, has been flown on high altitude aircraft. Results from simulations and from the airborne measurements are presented to demonstrate the revolutionary remote sounding capabilities to be realized with future satellite hyperspectral remote imaging/sounding systems.

Orbiting solar observatory 8 high resolution ultraviolet spectrometer experiment

NASA Technical Reports Server (NTRS)

1980-01-01

Oscillations, physical properties of the solar atmosphere, motions in the quiet solar atmosphere, coronal holes, motions in solar active regions, solar flares, the structure of plage regions, an atlas, and aeronomy are summarized. Photometric sensitivity, scattered light, ghosts, focus and spectral resolution, wavelength drive, photometric sensitivity, and scattered light, are also summarized. Experiments are described according to spacecraft made and experiment type. Some of the most useful data reduction programs are described.

Laboratory measurements of upwelled radiance and reflectance spectra of Calvert, Ball, Jordan, and Feldspar soil sediments

NASA Technical Reports Server (NTRS)

Whitlock, C. H.; Usry, J. W.; Witte, W. G.; Gurganus, E. A.

1977-01-01

An effort to investigate the potential of remote sensing for monitoring nonpoint source pollution was conducted. Spectral reflectance characteristics for four types of soil sediments were measured for mixture concentrations between 4 and 173 ppm. For measurements at a spectral resolution of 32 mm, the spectral reflectances of Calvert, Ball, Jordan, and Feldspar soil sediments were distinctly different over the wavelength range from 400 to 980 nm at each concentration tested. At high concentrations, spectral differences between the various sediments could be detected by measurements with a spectral resolution of 160 nm. At a low concentration, only small differences were observed between the various sediments when measurements were made with 160 nm spectral resolution. Radiance levels generally varied in a nonlinear manner with sediment concentration; linearity occurred in special cases, depending on sediment type, concentration range, and wavelength.

Compact Infrared Spectrometers

NASA Technical Reports Server (NTRS)

Mouroulis, Pantazis

2009-01-01

Concentric spectrometer forms are advantageous for constructing a variety of systems spanning the entire visible to infrared range. Spectrometer examples are given, including broadband or high resolution forms. Some issues associated with the Dyson catadioptric type are also discussed.

High resolution diffraction imaging of crystals grown in microgravity and closely related terrestrial crystals

NASA Technical Reports Server (NTRS)

Steiner, B.; Dobbyn, R.; Black, D.; Burdette, H.; Kuriyama, M.; Spal, R.; Vandenberg, L.; Fripp, A.; Simchick, R.; Lal, R.

1991-01-01

Irregularities found in three crystals grown in space, in four crystals grown entirely on the ground were examined and compared. Irregularities were observed in mercuric iodide, lead tin telluride, triglycine sulfate, and gallium arsenide by high resolution synchrotron x radiation diffraction imaging. Radiation detectors made from mercuric iodide crystals grown in microgravity were reported to perform far better than conventional detectors grown from the same material under full gravity. Effort is now underway to reproduce these 'space' crystals, optimize their properties, and extend comparable superiority to other types of materials.

Recent advances in small molecule OLED-on-silicon microdisplays

NASA Astrophysics Data System (ADS)

Ghosh, Amalkumar P.; Ali, Tariq A.; Khayrullin, Ilyas; Vazan, Fridrich; Prache, Olivier F.; Wacyk, Ihor

2009-08-01

High resolution OLED-on-silicon microdisplay technology is unique and challenging since it requires very small subpixel dimensions (~ 2-5 microns). eMagin's OLED microdisplay is based on white top emitter architecture using small molecule organic materials. The devices are fabricated using high Tg materials. The devices are hermetically sealed with vacuum deposited thin film layers. LCD-type color filters are patterned using photolithography methods to generate primary R, G, B colors. Results of recent improvements in the OLED-on-silicon microdisplay technology, with emphasis on efficiencies, lifetimes, grey scale and CIE color coordinates for SVGA and SXGA resolution microdisplays is presented.

CAFÉ-BEANS: An exhaustive hunt for high-mass binaries

NASA Astrophysics Data System (ADS)

Negueruela, I.; Maíz-Apellániz, J.; Simón-Díaz, S.; Alfaro, E. J.; Herrero, A.; Alonso, J.; Barbá, R.; Lorenzo, J.; Marco, A.; Monguió, M.; Morrell, N.; Pellerin, A.; Sota, A.; Walborn, N. R.

2015-05-01

CAFÉ-BEANS is an on-going survey running on the 2.2 m telescope at Calar Alto. For more than two years, CAFÉ-BEANS has been collecting high-resolution spectra of early-type stars with the aim of detecting and characterising spectroscopic binaries. The main goal of this project is a thorough characterisation of multiplicity in high-mass stars by detecting all spectroscopic and visual binaries in a large sample of Galactic O-type stars, and solving their orbits. Our final objective is eliminating all biases in the high-mass-star IMF created by undetected binaries.

Storage and utilization of HLA genomic data--new approaches to HLA typing.

PubMed

Helmberg, W

2000-01-01

Currently available DNA-based HLA typing assays can provide detailed information about sequence motifs of a tested sample. It is still a common practice, however, for information acquired by high-resolution sequence specific oligonucleotide probe (SSOP) typing or sequence specific priming (SSP) to be presented in a low-resolution serological format. Unfortunately, this representation can lead to significant loss of useful data in many cases. An alternative to assigning allele equivalents to suchDNA typing results is simply to store the observed typing pattern and utilize the information with the help of Virtual DNA Analysis (VDA). Interpretation of the stored typing patterns can then be updated based on newly defined alleles, assuming the sequence motifs detected by the typing reagents are known. Rather than updating reagent specificities in individual laboratories, such updates should be performed in a central, publicly available sequence database. By referring to this database, HLA genomic data can then be stored and transferred between laboratories without loss of information. The 13th International Histocompatibility Workshop offers an ideal opportunity to begin building this common database for the entire human MHC.

Detecting spatio-temporal changes in agricultural land use in Heilongjiang province, China using MODIS time-series data and a random forest regression model

NASA Astrophysics Data System (ADS)

Hu, Q.; Friedl, M. A.; Wu, W.

2017-12-01

Accurate and timely information regarding the spatial distribution of crop types and their changes is essential for acreage surveys, yield estimation, water management, and agricultural production decision-making. In recent years, increasing population, dietary shifts and climate change have driven drastic changes in China's agricultural land use. However, no maps are currently available that document the spatial and temporal patterns of these agricultural land use changes. Because of its short revisit period, rich spectral bands and global coverage, MODIS time series data has been shown to have great potential for detecting the seasonal dynamics of different crop types. However, its inherently coarse spatial resolution limits the accuracy with which crops can be identified from MODIS in regions with small fields or complex agricultural landscapes. To evaluate this more carefully and specifically understand the strengths and weaknesses of MODIS data for crop-type mapping, we used MODIS time-series imagery to map the sub-pixel fractional crop area for four major crop types (rice, corn, soybean and wheat) at 500-m spatial resolution for Heilongjiang province, one of the most important grain-production regions in China where recent agricultural land use change has been rapid and pronounced. To do this, a random forest regression (RF-g) model was constructed to estimate the percentage of each sub-pixel crop type in 2006, 2011 and 2016. Crop type maps generated through expert visual interpretation of high spatial resolution images (i.e., Landsat and SPOT data) were used to calibrate the regression model. Five different time series of vegetation indices (155 features) derived from different spectral channels of MODIS land surface reflectance (MOD09A1) data were used as candidate features for the RF-g model. An out-of-bag strategy and backward elimination approach was applied to select the optimal spectra-temporal feature subset for each crop type. The resulting crop maps were assessed in two ways: (1) wall-to-wall pixel comparison with corresponding high spatial resolution reference maps; and (2) county-level comparison with census data. Based on these derived maps, changes in crop type, total area, and spatial patterns of change in Heilongjiang province during 2006-2016 were analyzed.

Using whole genome sequencing to study American foulbrood epidemiology in honeybees

PubMed Central

Ågren, Joakim; Schäfer, Marc Oliver

2017-01-01

American foulbrood (AFB), caused by Paenibacillus larvae, is a devastating disease in honeybees. In most countries, the disease is controlled through compulsory burning of symptomatic colonies causing major economic losses in apiculture. The pathogen is endemic to honeybees world-wide and is readily transmitted via the movement of hive equipment or bees. Molecular epidemiology of AFB currently largely relies on placing isolates in one of four ERIC-genotypes. However, a more powerful alternative is multi-locus sequence typing (MLST) using whole-genome sequencing (WGS), which allows for high-resolution studies of disease outbreaks. To evaluate WGS as a tool for AFB-epidemiology, we applied core genome MLST (cgMLST) on isolates from a recent outbreak of AFB in Sweden. The high resolution of the cgMLST allowed different bacterial clones involved in the disease outbreak to be identified and to trace the source of infection. The source was found to be a beekeeper who had sold bees to two other beekeepers, proving the epidemiological link between them. No such conclusion could have been made using conventional MLST or ERIC-typing. This is the first time that WGS has been used to study the epidemiology of AFB. The results show that the technique is very powerful for high-resolution tracing of AFB-outbreaks. PMID:29140998

Earth Observatory Satellite system definition study. Report no. 5: System design and specifications. Part 2: Ground system element specifications

NASA Technical Reports Server (NTRS)

1974-01-01

The Ground System requirements for the Land Resources Management (LRM) type-A and type-B missions of the Earth Observatory Satellite (EOS) program are presented. Specifications for the Thematic Mapper data processing are provided (LRM A mission). The specifications also cover the R and D instruments (Thematic Mapper and High Resolution Pointable Imager) data processing for the LRM type-B mission.

High resolution molecular and histological analysis of renal disease progression in ZSF1 fa/faCP rats, a model of type 2 diabetic nephropathy

PubMed Central

Zhao, Shanrong; Schlerman, Franklin J.; Savary, Leigh; Campanholle, Gabriela; Johnson, Bryce G.; Xi, Li; Nguyen, Vuong; Zhan, Yutian; Lech, Matthew P.; Wang, Ju; Nie, Qing; Karsdal, Morten A.; Genovese, Federica; Boucher, Germaine; Brown, Thomas P.; Zhang, Baohong; Homer, Bruce L.; Martinez, Robert V.

2017-01-01

ZSF1 rats exhibit spontaneous nephropathy secondary to obesity, hypertension, and diabetes, and have gained interest as a model system with potentially high translational value to progressive human disease. To thoroughly characterize this model, and to better understand how closely it recapitulates human disease, we performed a high resolution longitudinal analysis of renal disease progression in ZSF1 rats spanning from early disease to end stage renal disease. Analyses included metabolic endpoints, renal histology and ultrastructure, evaluation of a urinary biomarker of fibrosis, and transcriptome analysis of glomerular-enriched tissue over the course of disease. Our findings support the translational value of the ZSF1 rat model, and are provided here to assist researchers in the determination of the model’s suitability for testing a particular mechanism of interest, the design of therapeutic intervention studies, and the identification of new targets and biomarkers for type 2 diabetic nephropathy. PMID:28746409

High resolution molecular and histological analysis of renal disease progression in ZSF1 fa/faCP rats, a model of type 2 diabetic nephropathy.

PubMed

Dower, Ken; Zhao, Shanrong; Schlerman, Franklin J; Savary, Leigh; Campanholle, Gabriela; Johnson, Bryce G; Xi, Li; Nguyen, Vuong; Zhan, Yutian; Lech, Matthew P; Wang, Ju; Nie, Qing; Karsdal, Morten A; Genovese, Federica; Boucher, Germaine; Brown, Thomas P; Zhang, Baohong; Homer, Bruce L; Martinez, Robert V

2017-01-01

ZSF1 rats exhibit spontaneous nephropathy secondary to obesity, hypertension, and diabetes, and have gained interest as a model system with potentially high translational value to progressive human disease. To thoroughly characterize this model, and to better understand how closely it recapitulates human disease, we performed a high resolution longitudinal analysis of renal disease progression in ZSF1 rats spanning from early disease to end stage renal disease. Analyses included metabolic endpoints, renal histology and ultrastructure, evaluation of a urinary biomarker of fibrosis, and transcriptome analysis of glomerular-enriched tissue over the course of disease. Our findings support the translational value of the ZSF1 rat model, and are provided here to assist researchers in the determination of the model's suitability for testing a particular mechanism of interest, the design of therapeutic intervention studies, and the identification of new targets and biomarkers for type 2 diabetic nephropathy.

A thin wideband high-spatial-resolution focusing metasurface for near-field passive millimeter-wave imaging

NASA Astrophysics Data System (ADS)

Chu, Hongjun; Qi, Jiaran; Xiao, Shanshan; Qiu, Jinghui

2018-04-01

In this paper, we present a flat transmission-type focusing metasurface for the near-field passive millimeter-wave (PMMW) imaging systems. Considering the non-uniform wavefront of the actual feeding horn, the metasurface is configured by unit cells consisting of coaxial annular apertures and is optimized to achieve broadband, high spatial resolution, and polarization insensitive properties important for PMMW imaging applications in the frequency range from 33 GHz to 37 GHz, with the focal spot as small as 0.43λ0 (@35 GHz). A prototype of the proposed metasurface is fabricated, and the measurement results fairly agree with the simulation ones. Furthermore, an experimental single-sensor PMMW imaging system is constructed based on the metasurface and a Ka-band direct detection radiometer. The experimental results show that the azimuth resolution of the system can reach approximately 4 mm (≈0.47λ0). It is shown that the proposed metasurface can potentially replace the bulky dielectric-lens or reflector antenna to achieve possibly more compact PMMW imaging systems with high spatial resolution approaching the diffraction-limit.

Fractionation of persistent organic pollutants in fish oil by high-performance liquid chromatography on a 2-(1-pyrenyl)ethyl silica column.

PubMed

Ortiz, X; Martí, R; Montaña, M J; Gasser, M; Margarit, L; Broto, F; Díaz-Ferrero, J

2010-09-01

The analysis of persistent organic pollutants in foodstuffs has become necessary for control of their levels in products for human and animal consumption. These analytical procedures usually require a fractionation step in order to separate the different families of pollutants to avoid interferences during the instrumental determination. In this study the separation was carried out on a 2-(1-pyrenyl)ethyl silica column, where analyte fractionation was based on differences in planarity and aromaticity. The fractionation of several types of persistent organic pollutants found in fish oil samples was studied; the pollutants included polychlorinated dibenzo-p-dioxins and dibenzofurans, polychlorinated biphenyls, polybrominated diphenyl ethers, and some organochlorine pesticides. Fractions were analyzed by high-resolution gas chromatography with electron-capture detection and high-resolution gas chromatography-high resolution mass spectroscopy. Finally, the whole method (including the purification, fractionation, and instrumental determination steps) was validated and successfully applied to the analysis of several samples of fish oil.

A high-sensitivity search for extraterrestrial intelligence at lambda 18 cm

NASA Technical Reports Server (NTRS)

Tarter, J.; Cuzzi, J.; Black, D.; Clark, T.

1980-01-01

A targeted high-sensitivity search for narrow-band signals near a wavelength of 18 cm has been conducted using the 91-m radiotelescope of the National Radio Astronomy Observatory. The search included 201 nearby solar-type stars and achieved a frequency resolution of 5.5 Hz over a 1.4-MHz bandwidth. This high spectral resolution was obtained through a non-real-time reduction procedure using a Mark I VLBI recording terminal in conjunction with the CDC 7600 computational facility at the NASA-Ames Research Center. This is the first high-resolution search for narrow-band signals in this wavelength regime. To date it is the most sensitive search per unit observing time of any search strategy which does not postulate a unique magic frequency. Data show no evidence for narrow-band signals due to extraterrestrial intelligence at a 12-standard-deviation upper limit on signal strength of 1.1 x 10 to the -23rd W/sq m.

High Resolution Radioluminescence Microscopy for the Study of Prostate Tissue Slice Cell Metabolism and Monitoring of Treatment Response

DTIC Science & Technology

2016-12-01

REPORT DATE : December 2016 TYPE OF REPORT: Final PREPARED FOR: U.S. Army Medical Research and Materiel Command Fort Detrick, Maryland 21702-5012...display a currently valid OMB control number. PLEASE DO NOT RETURN YOUR FORM TO THE ABOVE ADDRESS. 1. REPORT DATE (DD-MM-YYYY) December 2016 2. REPORT...TYPE Final 3. DATES COVERED (From - To) 30Sept2014 - 29Sept2016 4. TITLE AND SUBTITLE Monitoring of Treatment Response 5a. CONTRACT NUMBER High

pnCCD for photon detection from near-infrared to X-rays

NASA Astrophysics Data System (ADS)

Meidinger, Norbert; Andritschke, Robert; Hartmann, Robert; Herrmann, Sven; Holl, Peter; Lutz, Gerhard; Strüder, Lothar

2006-09-01

A pnCCD is a special type of charge-coupled device developed for spectroscopy and imaging of X-rays with high time resolution and quantum efficiency. Its most famous application is the operation on the XMM-Newton satellite, an X-ray astronomy mission that was launched by the European space agency in 1999. The excellent performance of the focal plane camera has been maintained for more than 6 years in orbit. The energy resolution in particular has shown hardly any degradation since launch. In order to satisfy the requirements of future X-ray astronomy missions as well as those of ground-based experiments, a new type of pnCCD has been developed. This ‘frame-store pnCCD’ shows an enhanced performance compared to the XMM-Newton type of pnCCD. Now, more options in device design and operation are available to tailor the detector to its respective application. Part of this concept is a programmable analog signal processor, which has been developed for the readout of the CCD signals. The electronic noise of the new detector has a value of only 2 electrons equivalent noise charge (ENC), which is less than half of the figure achieved for the XMM-Newton-type pnCCD. The energy resolution for the Mn-Kα line at 5.9 keV is approximately 130 eV FWHM. We have close to 100% quantum efficiency for both low- and high-energy photon detection (e.g. the C-K line at 277 eV, and the Ge-Kα line at 10 keV, respectively). Very high frame rates of 1000 images/s have been achieved due to the ultra-fast readout accomplished by the parallel architecture of the pnCCD and the analog signal processor. Excellent spectroscopic performance is shown even at the relatively high operating temperature of -25 °C that can be achieved by a Peltier cooler. The applications of the low-noise and fast pnCCD detector are not limited to the detection of X-rays. With an anti-reflective coating deposited on the photon entrance window, we achieve high quantum efficiency also for near-infrared and optical photons. A novel type of pnCCD is in preparation, which allows single optical photon counting. This feature is accomplished by implementation of an avalanche-type amplifier in the pnCCD concept.

Moderate-resolution spectral standards from lambda 5600 to lambda 9000

NASA Technical Reports Server (NTRS)

Allen, Lori E.; Strom, Karen M.

1995-01-01

We present a grid of stellar classification spectra of moderate resolution (R approximately 1500) in the range lambda lambda 5600-9000 A, compiled from high signal-to noise spectra of 275 stars, most in the open clusters Praesepe and M67. The grid covers dwarfs from types B8 through M5, giants from G8 through M7, and subgiants from F5 through K0. We catalog atomic and molecular absorption features useful for stellar classification, and demonstrate the use of luminosity-sensitive features to distinguish between late-type dwarf and giant stars. The entire database is made available in digital format on anonymous ftp and through the World Wide Web.

Electron spectroscopy for chemical analysis: Sample analysis

NASA Technical Reports Server (NTRS)

Carter, W. B.

1989-01-01

Exposure conditions in atomic oxygen (ESCA) was performed on an SSL-100/206 Small Spot Spectrometer. All data were taken with the use of a low voltage electron flood gun and a charge neutralization screen to minimize charging effects on the data. The X-ray spot size and electron flood gun voltage used are recorded on the individual spectra as are the instrumental resolutions. Two types of spectra were obtained for each specimen: (1) general surveys, and (2) high resolution spectra. The two types of data reduction performed are: (1) semiquantitative compositional analysis, and (2) peak fitting. The materials analyzed are: (1) kapton 4, 5, and 6, (2) HDPE 19, 20, and 21, and (3) PVDF 4, 5, and 6.

High-resolution abundance analysis of the metallic-line star HR 7250

NASA Astrophysics Data System (ADS)

Elmaslı, Aslı; Ünal, Kübraözge; Çalışkan, Şeyma

2018-07-01

We estimated the stellar parameters and chemical abundances of the highly neglected A-type star HR 7250. The star's high resolution spectrum, spanning a wavelength range from 3900 to 7900 Å, was obtained at the TÜBİTAK National Observatory. We derived the abundances of 14 elements (O, Na, Mg, Si, S, Ca, Sc, Ti, Cr, Fe, Ni, Sr, Y, and Ba) for HR 7250 from the unblended lines of the star's spectrum. Our analysis shows that HR 7250 is a chemically peculiar Am star. We also estimated its age and mass as 400 ± 70 Myr and 3.25 ± 0.17 M⊙ from evolutionary tracks and isochrones.

Spontaneous Raman scattering as a high resolution XUV radiation source

NASA Technical Reports Server (NTRS)

Rothenberg, J. E.; Young, J. F.; Harris, S. E.

1983-01-01

A type of high resolution XUV radiation source is described which is based upon spontaneous anti-Stokes scattering of tunable incident laser radiation from atoms excited to metastable levels. The theory of the source is summarized and two sets of experiments using He (1s2s)(1)S atoms, produced in a cw hollow cathode and in a pulsed high power microwave discharge, are discussed. The radiation source is used to examine transitions originating from the 3p(6) shell of potassium. The observed features include four previously unreported absorption lines and several sharp interferences of closely spaced autoionizing lines. A source linewidth of about 1.9 cm(-1) at 185,000 cm(-1) is demonstrated.

VizieR Online Data Catalog: Abundances in dwarfs, subgiants, and giants (da Silva+, 2015)

NASA Astrophysics Data System (ADS)

da Silva, R.; Milone, A. C.; Rocha-Pinto, H. J.

2015-05-01

Photospheric parameters mass, age, and the abundances of C, N, O, Na, Mg, Si, Ca, Ti, V, Mn, Fe, Ni, Cu, and Ba for a sample of FGK dwarfs, subgiants, and giants are derived. We used spectra of high-resolution (R~42,000) and high S/N (>150 on average) available in the ELODIE online database (Moultaka et al., 2004PASP..116..693M). These are spectra collected with the ELODIE high-resolution spectrograph (Baranne et al. 1996) of the Haute Provence Observatory (France). Only spectra with individual S/N>20 and with an image type classified as "object fibre only" (OBJO) were used. (7 data files).

Q: How do Microscopes Work?

ERIC Educational Resources Information Center

Zimov, Sarah

2004-01-01

Microscopes allow scientists to examine everyday objects in extraordinary ways. They provide high-resolution images that show objects in fine detail. This brief article describes the many types of microscopes and how they are used in different scientific venues.

A high-resolution method for the localization of proanthocyanidins in plant tissues

PubMed Central

2011-01-01

Background Histochemical staining of plant tissues with 4-dimethylaminocinnamaldehyde (DMACA) or vanillin-HCl is widely used to characterize spatial patterns of proanthocyanidin accumulation in plant tissues. These methods are limited in their ability to allow high-resolution imaging of proanthocyanidin deposits. Results Tissue embedding techniques were used in combination with DMACA staining to analyze the accumulation of proanthocyanidins in Lotus corniculatus (L.) and Trifolium repens (L.) tissues. Embedding of plant tissues in LR White or paraffin matrices, with or without DMACA staining, preserved the physical integrity of the plant tissues, allowing high-resolution imaging that facilitated cell-specific localization of proanthocyanidins. A brown coloration was seen in proanthocyanidin-producing cells when plant tissues were embedded without DMACA staining and this was likely to have been due to non-enzymatic oxidation of proanthocyanidins and the formation of colored semiquinones and quinones. Conclusions This paper presents a simple, high-resolution method for analysis of proanthocyanidin accumulation in organs, tissues and cells of two plant species with different patterns of proanthocyanidin accumulation, namely Lotus corniculatus (birdsfoot trefoil) and Trifolium repens (white clover). This technique was used to characterize cell type-specific patterns of proanthocyanidin accumulation in white clover flowers at different stages of development. PMID:21595992

Zebrafish skeleton development: High resolution micro-CT and FIB-SEM block surface serial imaging for phenotype identification

PubMed Central

Silvent, Jeremie; Akiva, Anat; Brumfeld, Vlad; Reznikov, Natalie; Rechav, Katya; Yaniv, Karina; Addadi, Lia; Weiner, Steve

2017-01-01

Although bone is one of the most studied living materials, many questions about the manner in which bones form remain unresolved, including fine details of the skeletal structure during development. In this study, we monitored skeleton development of zebrafish larvae, using calcein fluorescence, high-resolution micro-CT 3D images and FIB-SEM in the block surface serial imaging mode. We compared calcein staining of the skeletons of the wild type and nacre mutants, which are transparent zebrafish, with micro-CT for the first 30 days post fertilization embryos, and identified significant differences. We quantified the bone volumes and mineral contents of bones, including otoliths, during development, and showed that such developmental differences, including otolith development, could be helpful in identifying phenotypes. In addition, high-resolution imaging revealed the presence of mineralized aggregates in the notochord, before the formation of the first bone in the axial skeleton. These structures might play a role in the storage of the mineral. Our results highlight the potential of these high-resolution 3D approaches to characterize the zebrafish skeleton, which in turn could prove invaluable information for better understanding the development and the characterization of skeletal phenotypes. PMID:29220379

High Resolution Measurement of the Glycolytic Rate

PubMed Central

Bittner, Carla X.; Loaiza, Anitsi; Ruminot, Iván; Larenas, Valeria; Sotelo-Hitschfeld, Tamara; Gutiérrez, Robin; Córdova, Alex; Valdebenito, Rocío; Frommer, Wolf B.; Barros, L. Felipe

2010-01-01

The glycolytic rate is sensitive to physiological activity, hormones, stress, aging, and malignant transformation. Standard techniques to measure the glycolytic rate are based on radioactive isotopes, are not able to resolve single cells and have poor temporal resolution, limitations that hamper the study of energy metabolism in the brain and other organs. A new method is described in this article, which makes use of a recently developed FRET glucose nanosensor to measure the rate of glycolysis in single cells with high temporal resolution. Used in cultured astrocytes, the method showed for the first time that glycolysis can be activated within seconds by a combination of glutamate and K+, supporting a role for astrocytes in neurometabolic and neurovascular coupling in the brain. It was also possible to make a direct comparison of metabolism in neurons and astrocytes lying in close proximity, paving the way to a high-resolution characterization of brain energy metabolism. Single-cell glycolytic rates were also measured in fibroblasts, adipocytes, myoblasts, and tumor cells, showing higher rates for undifferentiated cells and significant metabolic heterogeneity within cell types. This method should facilitate the investigation of tissue metabolism at the single-cell level and is readily adaptable for high-throughput analysis. PMID:20890447

Two-dimensional dopant profiling of gallium nitride p-n junctions by scanning capacitance microscopy

NASA Astrophysics Data System (ADS)

Lamhamdi, M.; Cayrel, F.; Frayssinet, E.; Bazin, A. E.; Yvon, A.; Collard, E.; Cordier, Y.; Alquier, D.

2016-04-01

Two-dimensional imaging of dopant profiles for n and p-type regions are relevant for the development of new power semiconductors, especially for gallium nitride (GaN) for which classical profiling techniques are not adapted. This is a challenging task since it needs a technique with simultaneously good sensitivity, high spatial resolution and high dopant gradient resolution. To face these challenges, scanning capacitance microscopy combined with Atomic Force Microscopy is a good candidate, presenting reproducible results, as demonstrated in literature. In this work, we attempt to distinguish reliably and qualitatively the various doping concentrations and type at p-n and unipolar junctions. For both p-n and unipolar junctions three kinds of samples were prepared and measured separately. The space-charge region of the p-n metallurgical junction, giving rise to different contrasts under SCM imaging, is clearly observed, enlightening the interest of the SCM technique.

High-resolution analysis of locomotor activity rhythms in disconnected, a visual-system mutant of Drosophila melanogaster.

PubMed

Dowse, H B; Dushay, M S; Hall, J C; Ringo, J M

1989-07-01

Free-running locomotor activity and eclosion rhythms of Drosophila melanogaster, mutant at the disconnected (disco) locus, are substantially different from the wild-type phenotype. Initial periodogram analysis revealed little or no rhythmicity (Dushay et al., 1989). We have reanalyzed the locomotor activity data using high-resolution signal analysis (maximum-entropy spectral analysis, or MESA). These analyses, corroborated by autocorrelograms, uncovered significant residual circadian rhythmicity and strong ultradian rhythms in most of the animals tested. In this regard the disco mutants are much like flies expressing mutant alleles of the period gene, as well as wild-type flies reared throughout life in constant darkness. We hypothesize that light normally triggers the coupling of multiple ultradian oscillators into a functional circadian clock and that this process is disrupted in disco flies as a result of the neural lesion.

Sea Surface Wakes Observed by Spaceborne SAR in the Offshore Wind Farms

NASA Astrophysics Data System (ADS)

Li, Xiaoming; Lehner, Susanne; Jacobsen, Sven

2014-11-01

In the paper, we present some X-band spaceborne synthetic aperture radar (SAR) TerraSAR-X (TS-X) images acquired at the offshore wind farms in the North Sea and the East China Sea. The high spatial resolution SAR images show different sea surface wake patterns downstream of the offshore wind turbines. The analysis suggests that there are major two types of wakes among the observed cases. The wind turbine wakes generated by movement of wind around wind turbines are the most often observed cases. In contrast, due to the strong local tidal currents in the near shore wind farm sites, the tidal current wakes induced by tidal current impinging on the wind turbine piles are also observed in the high spatial resolution TS-X images. The discrimination of the two types of wakes observed in the offshore wind farms is also described in the paper.

Spectroscopy of bright Algol-type semi-detached close binary system HU Tauri (HR 1471)

NASA Astrophysics Data System (ADS)

Parthasarathy, M.

2018-01-01

Radial velocities of the primary component (B8V) of HU Tauri derived from the photographic spectra obtained during January 1974 to December 1974 and spectroscopic orbital elements from the analysis of the radial velocity curve of the B8V primary are given. The H line of the late type secondary component is clearly detected on the photographic spectra taken around the quadratures and radial velocities of the secondary component are derived. The radial velocity semi amplitudes of the primary (K) and secondary (K) are found to be 60 km/sec and 234 km/sec respectively. The mass ratio M/M = K/K is found to be 0.2564. The detection of the H line of the secondary is confirmed from the high resolution spectra that I obtained during 1981 and 1983 at quadratures using the 2.1-m McDonald observatory Otto Struve reflector telescope and high resolution coude Reticon spectrograph.

Image degradation in aerial imagery duplicates. [photographic processing of photographic film and reproduction (copying)

NASA Technical Reports Server (NTRS)

Lockwood, H. E.

1975-01-01

A series of Earth Resources Aircraft Program data flights were made over an aerial test range in Arizona for the evaluation of large cameras. Specifically, both medium altitude and high altitude flights were made to test and evaluate a series of color as well as black-and-white films. Image degradation, inherent in duplication processing, was studied. Resolution losses resulting from resolution characteristics of the film types are given. Color duplicates, in general, are shown to be degraded more than black-and-white films because of the limitations imposed by available aerial color duplicating stock. Results indicate that a greater resolution loss may be expected when the original has higher resolution. Photographs of the duplications are shown.

High-definition computed tomography for coronary artery stents imaging: Initial evaluation of the optimal reconstruction algorithm.

PubMed

Cui, Xiaoming; Li, Tao; Li, Xin; Zhou, Weihua

2015-05-01

The aim of this study was to evaluate the in vivo performance of four image reconstruction algorithms in a high-definition CT (HDCT) scanner with improved spatial resolution for the evaluation of coronary artery stents and intrastent lumina. Thirty-nine consecutive patients with a total of 71 implanted coronary stents underwent coronary CT angiography (CCTA) on a HDCT (Discovery CT 750 HD; GE Healthcare) with the high-resolution scanning mode. Four different reconstruction algorithms (HD-stand, HD-detail; HD-stand-plus; HD-detail-plus) were applied to reconstruct the stented coronary arteries. Image quality for stent characterization was assessed. Image noise and intrastent luminal diameter were measured. The relationship between the measurement of inner stent diameter (ISD) and the true stent diameter (TSD) and stent type were analysed. The stent-dedicated kernel (HD-detail) offered the highest percentage (53.5%) of good image quality for stent characterization and the highest ratio (68.0±8.4%) of visible stent lumen/true stent lumen for luminal diameter measurement at the expense of an increased overall image noise. The Pearson correlation coefficient between the ISD and TSD measurement and spearman correlation coefficient between the ISD measurement and stent type were 0.83 and 0.48, respectively. Compared with standard reconstruction algorithms, high-definition CT imaging technique with dedicated high-resolution reconstruction algorithm provides more accurate stent characterization and intrastent luminal diameter measurement. Copyright © 2015 Elsevier Ireland Ltd. All rights reserved.

Atmospheric absorption measurements in the region of 1 mm wavelength.

NASA Technical Reports Server (NTRS)

Emery, R.

1972-01-01

A Froome-type plasma-metal-junction device (1962) was used in high-resolution radiation transmission measurements in the atmosphere at wavelengths from 0.5 to 3.0 mm. The experimental and theoretical results for water vapor absorption lines in two submillimeter wavelength windows were compared, showing that this technique provided a much higher wavelength accuracy than more conventional optical-type spectroscopy.

Real-time high-resolution heterodyne-based measurements of spectral dynamics in fibre lasers

PubMed Central

Sugavanam, Srikanth; Fabbri, Simon; Le, Son Thai; Lobach, Ivan; Kablukov, Sergey; Khorev, Serge; Churkin, Dmitry

2016-01-01

Conventional tools for measurement of laser spectra (e.g. optical spectrum analysers) capture data averaged over a considerable time period. However, the generation spectrum of many laser types may involve spectral dynamics whose relatively fast time scale is determined by their cavity round trip period, calling for instrumentation featuring both high temporal and spectral resolution. Such real-time spectral characterisation becomes particularly challenging if the laser pulses are long, or they have continuous or quasi-continuous wave radiation components. Here we combine optical heterodyning with a technique of spatio-temporal intensity measurements that allows the characterisation of such complex sources. Fast, round-trip-resolved spectral dynamics of cavity-based systems in real-time are obtained, with temporal resolution of one cavity round trip and frequency resolution defined by its inverse (85 ns and 24 MHz respectively are demonstrated). We also show how under certain conditions for quasi-continuous wave sources, the spectral resolution could be further increased by a factor of 100 by direct extraction of phase information from the heterodyned dynamics or by using double time scales within the spectrogram approach. PMID:26984634

Impact of Mutation Type and Amplicon Characteristics on Genetic Diversity Measures Generated Using a High-Resolution Melting Diversity Assay

PubMed Central

Cousins, Matthew M.; Donnell, Deborah; Eshleman, Susan H.

2013-01-01

We adapted high-resolution melting (HRM) technology to measure genetic diversity without sequencing. Diversity is measured as a single numeric HRM score. Herein, we determined the impact of mutation types and amplicon characteristics on HRM diversity scores. Plasmids were generated with single-base changes, insertions, and deletions. Different primer sets were used to vary the position of mutations within amplicons. Plasmids and plasmid mixtures were analyzed to determine the impact of mutation type, position, and concentration on HRM scores. The impact of amplicon length and G/C content on HRM scores was also evaluated. Different mutation types affected HRM scores to varying degrees (1-bp deletion < 1-bp change < 3-bp insertion < 9-bp insertion). The impact of mutations on HRM scores was influenced by amplicon length and the position of the mutation within the amplicon. Mutations were detected at concentrations of 5% to 95%, with the greatest impact at 50%. The G/C content altered melting temperature values of amplicons but had no impact on HRM scores. These data are relevant to the design of assays that measure genetic diversity using HRM technology. PMID:23178437

TRMM Precipitation Radar Reflectivity Profiles Compared to High-Resolution Airborne and Ground-Based Radar Measurements

NASA Technical Reports Server (NTRS)

Heymsfield, G. M.; Geerts, B.; Tian, L.

1999-01-01

In this paper, TRMM (Tropical Rainfall Measuring Mission Satellite) Precipitation Radar (PR) products are evaluated by means of simultaneous comparisons with data from the high-altitude ER-2 Doppler Radar (EDOP), as well as ground-based radars. The comparison is aimed primarily at the vertical reflectivity structure, which is of key importance in TRMM rain type classification and latent heating estimation. The radars used in this study have considerably different viewing geometries and resolutions, demanding non-trivial mapping procedures in common earth-relative coordinates. Mapped vertical cross sections and mean profiles of reflectivity from the PR, EDOP, and ground-based radars are compared for six cases. These cases cover a stratiform frontal rainband, convective cells of various sizes and stages, and a hurricane. For precipitating systems that are large relative to the PR footprint size, PR reflectivity profiles compare very well to high-resolution measurements thresholded to the PR minimum reflectivity, and derived variables such as bright band height and rain types are accurate, even at high PR incidence angles. It was found that for, the PR reflectivity of convective cells small relative to the PR footprint is weaker than in reality. Some of these differences can be explained by non-uniform beam filling. For other cases where strong reflectivity gradients occur within a PR footprint, the reflectivity distribution is spread out due to filtering by the PR antenna illumination pattern. In these cases, rain type classification may err and be biased towards the stratiform type, and the average reflectivity tends to be underestimated. The limited sensitivity of the PR implies that the upper regions of precipitation systems remain undetected and that the PR storm top height estimate is unreliable, usually underestimating the actual storm top height. This applies to all cases but the discrepancy is larger for smaller cells where limited sensitivity is compounded by incomplete beam filling. Users of level three TRMM PR products should be aware of this scale dependency.

Influence of total beam current on HRTEM image resolution in differentially pumped ETEM with nitrogen gas.

PubMed

Bright, A N; Yoshida, K; Tanaka, N

2013-01-01

Environmental transmission electron microscopy (ETEM) enables the study of catalytic and other reaction processes as they occur with Angstrom-level resolution. The microscope used is a dedicated ETEM (Titan ETEM, FEI Company) with a differential pumping vacuum system and apertures, allowing aberration corrected high-resolution transmission electron microscopy (HRTEM) imaging to be performed with gas pressures up to 20 mbar in the sample area and with significant advantages over membrane-type E-cell holders. The effect on image resolution of varying the nitrogen gas pressure, electron beam current density and total beam current were measured using information limit (Young's fringes) on a standard cross grating sample and from silicon crystal lattice imaging. As expected, increasing gas pressure causes a decrease in HRTEM image resolution. However, the total electron beam current also causes big changes in the image resolution (lower beam current giving better resolution), whereas varying the beam current density has almost no effect on resolution, a result that has not been reported previously. This behavior is seen even with zero-loss filtered imaging, which we believe shows that the drop in resolution is caused by elastic scattering at gas ions created by the incident electron beam. Suitable conditions for acquiring high resolution images in a gas environment are discussed. Lattice images at nitrogen pressures up to 16 mbar are shown, with 0.12 nm information transfer at 4 mbar. Copyright © 2012 Elsevier B.V. All rights reserved.

Downscaling of Seasonal Landsat-8 and MODIS Land Surface Temperature (LST) in Kolkata, India

NASA Astrophysics Data System (ADS)

Garg, R. D.; Guha, S.; Mondal, A.; Lakshmi, V.; Kundu, S.

2017-12-01

The quality of life of urban people is affected by urban heat environment. The urban heat studies can be carried out using remotely sensed thermal infrared imagery for retrieving Land Surface Temperature (LST). Currently, high spatial resolution (<200 m) thermal images are limited and their temporal resolution is low (e.g., 17 days of Landsat-8). Coarse spatial resolution (1000 m) and high temporal resolution (daily) thermal images of MODIS (Moderate Resolution Imaging Spectroradiometer) are frequently available. The present study is to downscale spatially coarser resolution of the thermal image to fine resolution thermal image using regression based downscaling technique. This method is based on the relationship between (LST) and vegetation indices (e.g., Normalized Difference Vegetation Index or NDVI) over a heterogeneous landscape. The Kolkata metropolitan city, which experiences a tropical wet-and-dry type of climate has been selected for the study. This study applied different seasonal open source satellite images viz., Landsat-8 and Terra MODIS. The Landsat-8 images are aggregated at 960 m resolution and downscaled into 480, 240 120 and 60 m. Optical and thermal resolution of Landsat-8 and MODIS are 30 m and 60 m; 250 m and 1000 m respectively. The homogeneous land cover areas have shown better accuracy than heterogeneous land cover areas. The downscaling method plays a crucial role while the spatial resolution of thermal band renders it unable for advanced study. Key words: Land Surface Temperature (LST), Downscale, MODIS, Landsat, Kolkata

Aerosol Airmass Type Mapping Over the Urban Mexico City Region From Space-based Multi-angle Imaging

NASA Technical Reports Server (NTRS)

Patadia, F.; Kahn, R. A.; Limbacher, J. A.; Burton, S. P.; Ferrare, R. A.; Hostetler, C. A.; Hair, J. W.

2013-01-01

Using Multi-angle Imaging SpectroRadiometer (MISR) and sub-orbital measurements from the 2006 INTEX-B/MILAGRO field campaign, in this study we explore MISR's ability to map different aerosol air mass types over the Mexico City metropolitan area. The aerosol air mass distinctions are based on shape, size and single scattering albedo retrievals from the MISR Research Aerosol Retrieval algorithm. In this region, the research algorithm identifies dust-dominated aerosol mixtures based on non-spherical particle shape, whereas spherical biomass burning and urban pollution particles are distinguished by particle size. Two distinct aerosol air mass types based on retrieved particle microphysical properties, and four spatially distributed aerosol air masses, are identified in the MISR data on 6 March 2006. The aerosol air mass type identification results are supported by coincident, airborne high-spectral-resolution lidar (HSRL) measurements. Aerosol optical depth (AOD) gradients are also consistent between the MISR and sub-orbital measurements, but particles having single-scattering albedo of approx. 0.7 at 558 nm must be included in the retrieval algorithm to produce good absolute AOD comparisons over pollution-dominated aerosol air masses. The MISR standard V22 AOD product, at 17.6 km resolution, captures the observed AOD gradients qualitatively, but retrievals at this coarse spatial scale and with limited spherical absorbing particle options underestimate AOD and do not retrieve particle properties adequately over this complex urban region. However, we demonstrate how AOD and aerosol type mapping can be accomplished with MISR data over complex urban regions, provided the retrieval is performed at sufficiently high spatial resolution, and with a rich enough set of aerosol components and mixtures.

Countercurrent Chromatographic Separation of Proteins Using an Eccentric Coiled Column with Synchronous and Nonsynchronous Type-J Planetary Motions

PubMed Central

SHINOMIYA, Kazufusa; YOSHIDA, Kazunori; TOKURA, Koji; TSUKIDATE, Etsuhiro; YANAGIDAIRA, Kazuhiro; ITO, Yoichiro

2015-01-01

Protein separation was performed using the high-speed counter-current chromatograph (HSCCC) at both synchronous and nonsynchronous type-J planetary motions. The partition efficiency was evaluated with two different column configurations, eccentric coil and toroidal coil, on the separation of a set of stable protein samples including cytochrome C, myoglobin and lysozyme with a polymer phase system composed of 12.5% (w/w) polyethylene glycol 1000 and 12.5% (w/w) dibasic potassium phosphate. Better peak resolution was obtained by the eccentric coil than by the toroidal coil using either lower or upper phase as the mobile phase. The peak resolution was further improved using the eccentric coil by the nonsynchronous type-J planetary motion with the combination of 1066 rpm of column rotation and 1000 rpm of revolution. PMID:25765276

Countercurrent chromatographic separation of proteins using an eccentric coiled column with synchronous and nonsynchronous type-J planetary motions.

PubMed

Shinomiya, Kazufusa; Yoshida, Kazunori; Tokura, Koji; Tsukidate, Etsuhiro; Yanagidaira, Kazuhiro; Ito, Yoichiro

2015-01-01

Protein separation was performed using the high-speed countercurrent chromatograph (HSCCC) at both synchronous and nonsynchronous type-J planetary motions. The partition efficiency was evaluated with two different column configurations, eccentric coil and toroidal coil, on the separation of a set of stable protein samples including cytochrome C, myoglobin and lysozyme with a polymer phase system composed of 12.5% (w/w) polyethylene glycol 1000 and 12.5% (w/w) dibasic potassium phosphate. Better peak resolution was obtained by the eccentric coil than by the toroidal coil using either lower or upper phase as the mobile phase. The peak resolution was further improved using the eccentric coil by the nonsynchronous type-J planetary motion with the combination of 1066 rpm of column rotation and 1000 rpm of revolution.

Comparing High Definition Live Interactive and Store-and-Forward Consultations to In-Person Examinations

PubMed Central

Locatis, Craig; Burges, Gene; Maisiak, Richard; Liu, Wei-Li; Ackerman, Michael

2017-01-01

Abstract Background: There is little teledermatology research directly comparing remote methods, even less research with two in-person dermatologist agreement providing a baseline for comparing remote methods, and no research using high definition video as a live interactive method. Objective: To compare in-person consultations with store-and-forward and live interactive methods, the latter having two levels of image quality. Methods: A controlled study was conducted where patients were examined in-person, by high definition video, and by store-and-forward methods. The order patients experienced methods and residents assigned methods rotated, although an attending always saw patients in-person. The type of high definition video employed, lower resolution compressed or higher resolution uncompressed, was alternated between clinics. Primary and differential diagnoses, biopsy recommendations, and diagnostic and biopsy confidence ratings were recorded. Results: Concordance and confidence were significantly better for in-person versus remote methods and biopsy recommendations were lower. Store-and-forward and higher resolution uncompressed video results were similar and better than those for lower resolution compressed video. Limitations: Dermatology residents took store-and-forward photos and their quality was likely superior to those normally taken in practice. There were variations in expertise between the attending and second and third year residents. Conclusion: The superiority of in-person consultations suggests the tendencies to order more biopsies or still see patients in-person are often justified in teledermatology and that high resolution uncompressed video can close the resolution gap between store-and-forward and live interactive methods. PMID:27705083

Multishot versus Single-Shot Pulse Sequences in Very High Field fMRI: A Comparison Using Retinotopic Mapping

PubMed Central

Gatenby, J. Christopher; Gore, John C.; Tong, Frank

2012-01-01

High-resolution functional MRI is a leading application for very high field (7 Tesla) human MR imaging. Though higher field strengths promise improvements in signal-to-noise ratios (SNR) and BOLD contrast relative to fMRI at 3 Tesla, these benefits may be partially offset by accompanying increases in geometric distortion and other off-resonance effects. Such effects may be especially pronounced with the single-shot EPI pulse sequences typically used for fMRI at standard field strengths. As an alternative, one might consider multishot pulse sequences, which may lead to somewhat lower temporal SNR than standard EPI, but which are also often substantially less susceptible to off-resonance effects. Here we consider retinotopic mapping of human visual cortex as a practical test case by which to compare examples of these sequence types for high-resolution fMRI at 7 Tesla. We performed polar angle retinotopic mapping at each of 3 isotropic resolutions (2.0, 1.7, and 1.1 mm) using both accelerated single-shot 2D EPI and accelerated multishot 3D gradient-echo pulse sequences. We found that single-shot EPI indeed led to greater temporal SNR and contrast-to-noise ratios (CNR) than the multishot sequences. However, additional distortion correction in postprocessing was required in order to fully realize these advantages, particularly at higher resolutions. The retinotopic maps produced by both sequence types were qualitatively comparable, and showed equivalent test/retest reliability. Thus, when surface-based analyses are planned, or in other circumstances where geometric distortion is of particular concern, multishot pulse sequences could provide a viable alternative to single-shot EPI. PMID:22514646

Quantification of in vivo colonic motor patterns in healthy humans before and after a meal revealed by high-resolution fiber-optic manometry

PubMed Central

DINNING, P. G.; WIKLENDT, L.; MASLEN, L.; GIBBINS, I.; PATTON, V.; ARKWRIGHT, J. W.; LUBOWSKI, D. Z.; O'GRADY, G.; BAMPTON, P. A.; BROOKES, S. J.; COSTA, M.

2015-01-01

Background Until recently, investigations of the normal patterns of motility of the healthy human colon have been limited by the resolution of in vivo recording techniques. Methods We have used a new, high-resolution fiber-optic manometry system (72 sensors at 1-cm intervals) to record motor activity from colon in 10 healthy human subjects. Key Results In the fasted colon, on the basis of rate and extent of propagation, four types of propagating motor pattern could be identified: (i) cyclic motor patterns (at 2–6/min); (ii) short single motor patterns; (iii) long single motor patterns; and (iv) occasional retrograde, slow motor patterns. For the most part, the cyclic and short single motor patterns propagated in a retrograde direction. Following a 700 kCal meal, a fifth motor pattern appeared; high-amplitude propagating sequences (HAPS) and there was large increase in retrograde cyclic motor patterns (5.6±5.4/2 h vs 34.7±19.8/2 h; p < 0.001). The duration and amplitude of individual pressure events were significantly correlated. Discriminant and multivariate analysis of duration, gradient, and amplitude of the pressure events that made up propagating motor patterns distinguished clearly two types of pressure events: those belonging to HAPS and those belonging to all other propagating motor patterns. Conclusions & Inferences This work provides the first comprehensive description of colonic motor patterns recorded by high-resolution manometry and demonstrates an abundance of retrograde propagating motor patterns. The propagating motor patterns appear to be generated by two independent sources, potentially indicating their neurogenic or myogenic origin. PMID:25131177

Multishot versus single-shot pulse sequences in very high field fMRI: a comparison using retinotopic mapping.

PubMed

Swisher, Jascha D; Sexton, John A; Gatenby, J Christopher; Gore, John C; Tong, Frank

2012-01-01

High-resolution functional MRI is a leading application for very high field (7 Tesla) human MR imaging. Though higher field strengths promise improvements in signal-to-noise ratios (SNR) and BOLD contrast relative to fMRI at 3 Tesla, these benefits may be partially offset by accompanying increases in geometric distortion and other off-resonance effects. Such effects may be especially pronounced with the single-shot EPI pulse sequences typically used for fMRI at standard field strengths. As an alternative, one might consider multishot pulse sequences, which may lead to somewhat lower temporal SNR than standard EPI, but which are also often substantially less susceptible to off-resonance effects. Here we consider retinotopic mapping of human visual cortex as a practical test case by which to compare examples of these sequence types for high-resolution fMRI at 7 Tesla. We performed polar angle retinotopic mapping at each of 3 isotropic resolutions (2.0, 1.7, and 1.1 mm) using both accelerated single-shot 2D EPI and accelerated multishot 3D gradient-echo pulse sequences. We found that single-shot EPI indeed led to greater temporal SNR and contrast-to-noise ratios (CNR) than the multishot sequences. However, additional distortion correction in postprocessing was required in order to fully realize these advantages, particularly at higher resolutions. The retinotopic maps produced by both sequence types were qualitatively comparable, and showed equivalent test/retest reliability. Thus, when surface-based analyses are planned, or in other circumstances where geometric distortion is of particular concern, multishot pulse sequences could provide a viable alternative to single-shot EPI.

The Bidirectional Reflectance Distribution Function and Albedo Analysis for Various Land-Cover Types in the Midwestern United States for the Tempo Satellite

NASA Astrophysics Data System (ADS)

Marshall, Bethany

The Tropospheric Emissions: Monitoring of Pollution (TEMPO) satellite, scheduled to be launched around the year 2020, is the first phase of NASA's next generation of missions that will study the composition of the Earth's atmosphere. TEMPO provides a new set of high-resolution ( 0.4 nm) spectral data in the ultraviolet and visible portion of the electromagnetic spectrum that can be used to measure atmospheric pollutants. Due to its high spectral resolution and hourly temporal resolution covering entire North America, TEMPO data can also be used along with the calculation of spectral indices using the bidirectional reflection distribution functions (BRDF) or albedo to improve crop growth and yield monitoring for regional food security. The objectives of this thesis research were to 1) characterize BRDF/albedo of various land-cover types in Midwestern United States that can be used to remove land surface competent from at-sensor TEMPO radiances for accurate estimation of atmospheric chemistry and 2) evaluation of TEMPO data for regional agro-ecosystem studies. To this end, we: (1) collected 461 upwelling and downwelling solar irradiances and spectral albedo of various land-cover types (e.g., grapevine, maize, soybean, tomato, rock, asphalt road and concrete pave way, clean and turbid waters) at 110 sites in the States of Missouri, Illinois, Indiana, and Colorado using a PSR-3500 hand-held Spectroradiometer; (2) conducted a field and manned aircraft data collection campaign in Maryland Heights, Missouri using the Geo-TASO flight instrument flown onboard the NASA HU-25C Falcon aircraft on August 13, 2014; and (3) utilized Ross-Li Kernel BRDF model and MODTRAN radiative transfer simulations to characterize BRDF/albedo of various land-cover types. TEMPO retrieval of atmospheric gases must account for the effects of surface BRDF/albedo. Since BRDF is an inherent optical properties of surface, this research will contribute to the TEMPO mission by providing high-resolution spectral BRDF/albedo database, which may also promote the use of the TEMPO data for agricultural and ecosystem monitoring.

Source Identification of PM2.5 in Steubenville, Ohio Using a Hybrid Method for Highly Time-resolved Data

EPA Science Inventory

A new source-type identification method, Reduction and Species Clustering Using Episodes (ReSCUE), was developed to exploit the temporal synchronicity between species to form clusters of species that vary together. High time-resolution (30 min) PM_2.5 sampling was condu...

Grazing Incidence Nickel Replicated Optics for Hard X-ray Telescopes

NASA Technical Reports Server (NTRS)

Peturzzo, J. J., III; Elsner, R. F.; Joy, M. K.; ODell, S. L.; Weisskopf, M. C.

1997-01-01

The requirements for future hard x-ray (up to 50 keV) telescopes are lightweight, high angular resolution optics with large collecting areas. Grazing incidence replicated optics are an excellent candidate for this, type of mission, providing better angular resolution, comparable area/unit mass, and simpler fabrication than multilayer-coated foils. Most importantly, the technology to fabricate the required optics currently exists. A comparison of several hard x-ray telescope designs will be presented.

Atomic resolution structure of cucurmosin, a novel type 1 ribosome-inactivating protein from the sarcocarp of Cucurbita moschata

DOE Office of Scientific and Technical Information (OSTI.GOV)

Hou, Xiaomin; Meehan, Edward J.; Xie, Jieming

2008-10-27

A novel type 1 ribosome-inactivating protein (RIP) designated cucurmosin was isolated from the sarcocarp of Cucurbita moschata (pumpkin). Besides rRNA N-glycosidase activity, cucurmosin exhibits strong cytotoxicities to three cancer cell lines of both human and murine origins, but low toxicity to normal cells. Plant genomic DNA extracted from the tender leaves was amplified by PCR between primers based on the N-terminal sequence and X-ray sequence of the C-terminal. The complete mature protein sequence was obtained from N-terminal protein sequencing and partial DNA sequencing, confirmed by high resolution crystal structure analysis. The crystal structure of cucurmosin has been determined at 1.04more » {angstrom}, a resolution that has never been achieved before for any RIP. The structure contains two domains: a large N-terminal domain composed of seven {alpha}-helices and eight {beta}-strands, and a smaller C-terminal domain consisting of three {alpha}-helices and two {beta}-strands. The high resolution structure established a glycosylation pattern of GlcNAc{sub 2}Man3Xyl. Asn225 was identified as a glycosylation site. Residues Tyr70, Tyr109, Glu158 and Arg161 define the active site of cucurmosin as an RNA N-glycosidase. The structural basis of cytotoxicity difference between cucurmosin and trichosanthin is discussed.« less

Kinetic and dynamic kinetic resolution of secondary alcohols with ionic-surfactant-coated Burkholderia cepacia lipase: substrate scope and enantioselectivity.

PubMed

Kim, Cheolwoo; Lee, Jusuk; Cho, Jeonghun; Oh, Yeonock; Choi, Yoon Kyung; Choi, Eunjeong; Park, Jaiwook; Kim, Mahn-Joo

2013-03-15

Forty-four different secondary alcohols, which can be classified into several types (II-IX), were tested as the substrates of ionic surfactant-coated Burkholderia cepacia lipase (ISCBCL) to see its substrate scope and enantioselectivity in kinetic and dynamic kinetic resolution (KR and DKR). They include 6 boron-containing alcohols, 24 chiral propargyl alcohols, and 14 diarylmethanols. The results from the studies on KR indicate that ISCBCL accepted most of them with high enantioselectivity at ambient temperature and with useful to high enantioselectivity at elevated temperatures. In particular, ISCBCL displayed high enantioselectivity toward sterically demanding secondary alcohols (types VIII and IX) which have two bulky substituents at the hydroxymethine center. DKR reactions were performed by the combination of ISCBCL with a ruthenium-based racemization catalyst at 25-60 °C. Forty-one secondary alcohols were tested for DKR. About half of them were transformed into their acetates of high enantiopurity (>90% ee) with good yields (>80%). It is concluded that ISCBCL appears to be a superb enzyme for the KR and DKR of secondary alcohols.

Quantum Computers

DTIC Science & Technology

2010-03-04

and their sensitivity to charge and flux fluctuations. The first type of superconducting qubit , the charge qubit , omits the inductance . There is no...nanostructured NbN superconducting nanowire detectors have achieved high efficiency and photon number resolution16,17. One approach to a high-efficiency single...resemble classical high- speed integrated circuits and can be readily fabricated using existing technologies. The basic physics behind superconducting qubits

High-resolution melting genotyping of Enterococcus faecium based on multilocus sequence typing derived single nucleotide polymorphisms.

PubMed

Tong, Steven Y C; Xie, Shirley; Richardson, Leisha J; Ballard, Susan A; Dakh, Farshid; Grabsch, Elizabeth A; Grayson, M Lindsay; Howden, Benjamin P; Johnson, Paul D R; Giffard, Philip M

2011-01-01

We have developed a single nucleotide polymorphism (SNP) nucleated high-resolution melting (HRM) technique to genotype Enterococcus faecium. Eight SNPs were derived from the E. faecium multilocus sequence typing (MLST) database and amplified fragments containing these SNPs were interrogated by HRM. We tested the HRM genotyping scheme on 85 E. faecium bloodstream isolates and compared the results with MLST, pulsed-field gel electrophoresis (PFGE) and an allele specific real-time PCR (AS kinetic PCR) SNP typing method. In silico analysis based on predicted HRM curves according to the G+C content of each fragment for all 567 sequence types (STs) in the MLST database together with empiric data from the 85 isolates demonstrated that HRM analysis resolves E. faecium into 231 "melting types" (MelTs) and provides a Simpson's Index of Diversity (D) of 0.991 with respect to MLST. This is a significant improvement on the AS kinetic PCR SNP typing scheme that resolves 61 SNP types with D of 0.95. The MelTs were concordant with the known ST of the isolates. For the 85 isolates, there were 13 PFGE patterns, 17 STs, 14 MelTs and eight SNP types. There was excellent concordance between PFGE, MLST and MelTs with Adjusted Rand Indices of PFGE to MelT 0.936 and ST to MelT 0.973. In conclusion, this HRM based method appears rapid and reproducible. The results are concordant with MLST and the MLST based population structure.

Detection of α-thalassemia-1 Southeast Asian and Thai Type Deletions and β-thalassemia 3.5-kb Deletion by Single-tube Multiplex Real-time PCR with SYBR Green1 and High-resolution Melting Analysis

PubMed Central

Wiengkum, Thanatcha; Srithep, Sarinee; Chainoi, Isarapong; Singboottra, Panthong; Wongwiwatthananukit, Sanchai

2011-01-01

Background Prevention and control of thalassemia requires simple, rapid, and accurate screening tests for carrier couples who are at risk of conceiving fetuses with severe thalassemia. Methods Single-tube multiplex real-time PCR with SYBR Green1 and high-resolution melting (HRM) analysis were used for the identification of α-thalassemia-1 Southeast Asian (SEA) and Thai type deletions and β-thalassemia 3.5-kb gene deletion. The results were compared with those obtained using conventional gap-PCR. DNA samples were derived from 28 normal individuals, 11 individuals with α-thalassemia-1 SEA type deletion, 2 with α-thalassemia-1 Thai type deletion, and 2 with heterozygous β-thalassemia 3.5-kb gene deletion. Results HRM analysis indicated that the amplified fragments from α-thalassemia-1 SEA type deletion, α-thalassemia-1 Thai type deletion, β-thalassemia 3.5-kb gene deletion, and the wild-type β-globin gene had specific peak heights at mean melting temperature (Tm) values of 86.89℃, 85.66℃, 77.24℃, and 74.92℃, respectively. The results obtained using single-tube multiplex real-time PCR with SYBR Green1 and HRM analysis showed 100% consistency with those obtained using conventional gap-PCR. Conclusions Single-tube multiplex real-time PCR with SYBR Green1 and HRM analysis is a potential alternative for routine clinical screening of the common types of α- and β-thalassemia large gene deletions, since it is simple, cost-effective, and highly accurate. PMID:21779184

A polychromator-type near-infrared spectrometer with a high-sensitivity and high-resolution photodiode array detector for pharmaceutical process monitoring on the millisecond time scale.

PubMed

Murayama, Kodai; Genkawa, Takuma; Ishikawa, Daitaro; Komiyama, Makoto; Ozaki, Yukihiro

2013-02-01

In the fine chemicals industry, particularly in the pharmaceutical industry, advanced sensing technologies have recently begun being incorporated into the process line in order to improve safety and quality in accordance with process analytical technology. For estimating the quality of powders without preparation during drug formulation, near-infrared (NIR) spectroscopy has been considered the most promising sensing approach. In this study, we have developed a compact polychromator-type NIR spectrometer equipped with a photodiode (PD) array detector. This detector is consisting of 640 InGaAs-PD elements with 20-μm pitch. Some high-specification spectrometers, which use InGaAs-PD with 512 elements, have a wavelength resolution of about 1.56 nm when covering 900-1700 nm range. On the other hand, the newly developed detector, having the PD with one of the world's highest density, enables wavelength resolution of below 1.25 nm. Moreover, thanks to the combination with a highly integrated charge amplifier array circuit, measurement speed of the detector is higher by two orders than that of existing PD array detectors. The developed spectrometer is small (120 mm × 220 mm × 200 mm) and light (6 kg), and it contains various key devices including the high-density and high-sensitivity PD array detector, NIR technology, and spectroscopy technology for a spectroscopic analyzer that has the required detection mechanism and high sensitivity for powder measurement, as well as a high-speed measuring function for blenders. Moreover, we have evaluated the characteristics of the developed NIR spectrometer, and the measurement of powder samples confirmed that it has high functionality.

Correlation between Preoperative High Resolution Computed Tomography (CT) Findings with Surgical Findings in Chronic Otitis Media (COM) Squamosal Type.

PubMed

Karki, S; Pokharel, M; Suwal, S; Poudel, R

Background The exact role of High resolution computed tomography (HRCT) temporal bone in preoperative assessment of Chronic suppurative otitis media atticoantral disease still remains controversial. Objective To evaluate the role of high resolution computed tomography temporal bone in Chronic suppurative otitis media atticoantral disease and to compare preoperative computed tomographic findings with intra-operative findings. Method Prospective, analytical study conducted among 65 patients with chronic suppurative otitis media atticoantral disease in Department of Radiodiagnosis, Kathmandu University Dhulikhel Hospital between January 2015 to July 2016. The operative findings were compared with results of imaging. The parameters of comparison were erosion of ossicles, scutum, facial canal, lateral semicircular canal, sigmoid and tegmen plate along with extension of disease to sinus tympani and facial recess. Sensitivity, specificity, negative predictive value, positive predictive values were calculated. Result High resolution computed tomography temporal bone offered sensitivity (Se) and specificity (Sp) of 100% for visualization of sigmoid and tegmen plate erosion. The performance of HRCT in detecting malleus (Se=100%, Sp=95.23%), incus (Se=100%,Sp=80.48%) and stapes (Se=96.55%, Sp=71.42%) erosion was excellent. It offered precise information about facial canal erosion (Se=100%, Sp=75%), scutum erosion (Se=100%, Sp=96.87%) and extension of disease to facial recess and sinus tympani (Se=83.33%,Sp=100%). high resolution computed tomography showed specificity of 100% for lateral semicircular canal erosion (Sp=100%) but with low sensitivity (Se=53.84%). Conclusion The findings of high resolution computed tomography and intra-operative findings were well comparable except for lateral semicircular canal erosion. high resolution computed tomography temporal bone acts as a road map for surgeon to identify the extent of disease, plan for appropriate procedure that is required and prepare for potential complications that can be encountered during surgery.

Mapping Chinese tallow with color-infrared photography

USGS Publications Warehouse

Ramsey, Elijah W.; Nelson, G.A.; Sapkota, S.K.; Seeger, E.B.; Martella, K.D.

2002-01-01

Airborne color-infrared photography (CIR) (1:12,000 scale) was used to map localized occurrences of the widespread and aggressive Chinese tallow (Sapium sebiferum), an invasive species. Photography was collected during senescence when Chinese tallow's bright red leaves presented a high spectral contrast within the native bottomland hardwood and upland forests and marsh land-cover types. Mapped occurrences were conservative because not all senescing tallow leaves are bright red simultaneously. To simulate low spectral but high spatial resolution satellite/airborne image and digital video data, the CIR photography was transformed into raster images at spatial resolutions approximating 0.5 in and 1.0 m. The image data were then spectrally classified for the occurrence of bright red leaves associated with senescing Chinese tallow. Classification accuracies were greater than 95 percent at both spatial resolutions. There was no significant difference in either forest in the detection of tallow or inclusion of non-tallow trees associated with the two spatial resolutions. In marshes, slightly more tallow occurrences were mapped with the lower spatial resolution, but there were also more misclassifications of native land covers as tallow. Combining all land covers, there was no difference at detecting tallow occurrences (equal omission errors) between the two resolutions, but the higher spatial resolution was associated with less inclusion of non-tallow land covers as tallow (lower commission error). Overall, these results confirm that high spatial (???1 m) but low spectral resolution remote sensing data can be used for mapping Chinese tallow trees in dominant environments found in coastal and adjacent upland landscapes.

Genotyping applications for transplantation and transfusion management: The Emory Experience

PubMed Central

Fasano, Ross M.; Sullivan, Harold Cliff; Bray, Bob; Gebel, Howie; Meyer, Erin K.; Winkler, Annie M.; Josephson, Cassandra D.; Stowell, Sean R.; Duncan, Sandy; Roback, John D.

2018-01-01

Current genotyping methodologies for transplantation and transfusion management employ multiplex systems that allow for the simultaneous detection of multiple human leukocyte antigens (HLA), human platelet antigens (HPA) and red blood cell (RBC) antigens. The development of high resolution molecular HLA typing has led to improved outcomes of unrelated hematopoietic stem cell transplants by better identifying suitable donors typed at the allele level for HLA-A, B, C, DRB1 and DQB1 antigens. In solid organ transplantation, the combination of high resolution HLA typing along with solid-phase antibody identification and the calculated PRA have shown to be of specific benefit to highly sensitized patients, and have resulted in significant reductions of incompatible crossmatches at the time of organ allocation. This database-driven combined HLA antigen/antibody testing has promoted the routine implementation of the virtual crossmatch, in which an electronic crossmatch is performed, and perhaps even obviates the need for a physical crossmatch. Additionally, DNA-based testing for RBC antigens provides as an alternative typing method that mitigates many of the limitations of hemagglutination-based phenotyping. Although there are many applications of RBC genotyping in various transfusion settings, it has arguably been most useful in the management of transfusion-dependent patients with sickle cell disease (SCD) and thalassemia to minimize alloimmunization. The availability of high-throughput RBC genotyping for both patients and large populations of donors, along with coordinated informatics systems to link patients’ antigen needs with available antigen-negative and/or rare blood-typed donors, offer promise toward improving the efficiency, reliability, and extent of RBC matching for this population. PMID:28234571

Effects of Reduced Terrestrial LiDAR Point Density on High-Resolution Grain Crop Surface Models in Precision Agriculture

PubMed Central

Hämmerle, Martin; Höfle, Bernhard

2014-01-01

3D geodata play an increasingly important role in precision agriculture, e.g., for modeling in-field variations of grain crop features such as height or biomass. A common data capturing method is LiDAR, which often requires expensive equipment and produces large datasets. This study contributes to the improvement of 3D geodata capturing efficiency by assessing the effect of reduced scanning resolution on crop surface models (CSMs). The analysis is based on high-end LiDAR point clouds of grain crop fields of different varieties (rye and wheat) and nitrogen fertilization stages (100%, 50%, 10%). Lower scanning resolutions are simulated by keeping every n-th laser beam with increasing step widths n. For each iteration step, high-resolution CSMs (0.01 m2 cells) are derived and assessed regarding their coverage relative to a seamless CSM derived from the original point cloud, standard deviation of elevation and mean elevation. Reducing the resolution to, e.g., 25% still leads to a coverage of >90% and a mean CSM elevation of >96% of measured crop height. CSM types (maximum elevation or 90th-percentile elevation) react differently to reduced scanning resolutions in different crops (variety, density). The results can help to assess the trade-off between CSM quality and minimum requirements regarding equipment and capturing set-up. PMID:25521383

High resolution weather data for urban hydrological modelling and impact assessment, ICT requirements and future challenges

NASA Astrophysics Data System (ADS)

ten Veldhuis, Marie-claire; van Riemsdijk, Birna

2013-04-01

Hydrological analysis of urban catchments requires high resolution rainfall and catchment information because of the small size of these catchments, high spatial variability of the urban fabric, fast runoff processes and related short response times. Rainfall information available from traditional radar and rain gauge networks does no not meet the relevant scales of urban hydrology. A new type of weather radars, based on X-band frequency and equipped with Doppler and dual polarimetry capabilities, promises to provide more accurate rainfall estimates at the spatial and temporal scales that are required for urban hydrological analysis. Recently, the RAINGAIN project was started to analyse the applicability of this new type of radars in the context of urban hydrological modelling. In this project, meteorologists and hydrologists work closely together in several stages of urban hydrological analysis: from the acquisition procedure of novel and high-end radar products to data acquisition and processing, rainfall data retrieval, hydrological event analysis and forecasting. The project comprises of four pilot locations with various characteristics of weather radar equipment, ground stations, urban hydrological systems, modelling approaches and requirements. Access to data processing and modelling software is handled in different ways in the pilots, depending on ownership and user context. Sharing of data and software among pilots and with the outside world is an ongoing topic of discussion. The availability of high resolution weather data augments requirements with respect to the resolution of hydrological models and input data. This has led to the development of fully distributed hydrological models, the implementation of which remains limited by the unavailability of hydrological input data. On the other hand, if models are to be used in flood forecasting, hydrological models need to be computationally efficient to enable fast responses to extreme event conditions. This presentation will highlight ICT-related requirements and limitations in high resolution urban hydrological modelling and analysis. Further ICT challenges arise in provision of high resolution radar data for diverging information needs as well as in combination with other data sources in the urban environment. Different types of information are required for such diverse activities as operational flood protection, traffic management, large event organisation, business planning in shopping districts and restaurants, timing of family activities. These different information needs may require different configurations and data processing for radars and other data sources. An ICT challenge is to develop techniques for deciding how to automatically respond to these diverging information needs (e.g., through (semi-)automated negotiation). Diverse activities also provide a wide variety of information resources that can supplement traditional networks of weather sensors, such as rain sensors on cars and social media. Another ICT challenge is how to combine data from these different sources for answering a particular information need. Examples will be presented of solutions are currently being explored.

High resolution FTIR spectroscopy of the ν7 band of CD3CCH

NASA Astrophysics Data System (ADS)

Pal, Ayan Kumar; Kshirsagar, R. J.

2018-03-01

The high-resolution Fourier transform spectrum of propyne-d3 (CD3CCH) at room temperature has been recorded in the region of the ν7 band (950-1200 cm-1) at an apodized resolution of 0.004 cm-1. About 2400 lines consisting of a total of 25 sub-bands ranging from KΔK = -13 to 12 have been assigned in the ν7 band of CD3CCH. In the fitting analysis, the ν4 = 1 state to which transitions have not been identified in the experimental spectrum included as a "shadow" state. The data have been analyzed taking into account of the strong x-y Coriolis interaction of the ν7 = 1 state with the ν4 = 1 state. l-type interactions between the ± l components of the ν7 = 1 state, and a weak k-type doubling interaction between ν7 = 1 and ν4 = 1 states have been included in the analysis. The vibration-rotation transitions for K ≥ 8 show fairly large amount of deviation and most likely interacted by other nearby states. The transitions upto K = 7 and Jmax = 61 could be fitted with a standard deviation of 0.0007 cm-1.

JIGSAW-GEO (1.0): Locally Orthogonal Staggered Unstructured Grid Generation for General Circulation Modelling on the Sphere

NASA Technical Reports Server (NTRS)

Engwirda, Darren

2017-01-01

An algorithm for the generation of non-uniform, locally orthogonal staggered unstructured spheroidal grids is described. This technique is designed to generate very high-quality staggered VoronoiDelaunay meshes appropriate for general circulation modelling on the sphere, including applications to atmospheric simulation, ocean-modelling and numerical weather prediction. Using a recently developed Frontal-Delaunay refinement technique, a method for the construction of high-quality unstructured spheroidal Delaunay triangulations is introduced. A locally orthogonal polygonal grid, derived from the associated Voronoi diagram, is computed as the staggered dual. It is shown that use of the Frontal-Delaunay refinement technique allows for the generation of very high-quality unstructured triangulations, satisfying a priori bounds on element size and shape. Grid quality is further improved through the application of hill-climbing-type optimisation techniques. Overall, the algorithm is shown to produce grids with very high element quality and smooth grading characteristics, while imposing relatively low computational expense. A selection of uniform and non-uniform spheroidal grids appropriate for high-resolution, multi-scale general circulation modelling are presented. These grids are shown to satisfy the geometric constraints associated with contemporary unstructured C-grid-type finite-volume models, including the Model for Prediction Across Scales (MPAS-O). The use of user-defined mesh-spacing functions to generate smoothly graded, non-uniform grids for multi-resolution-type studies is discussed in detail.

JIGSAW-GEO (1.0): locally orthogonal staggered unstructured grid generation for general circulation modelling on the sphere

NASA Astrophysics Data System (ADS)

Engwirda, Darren

2017-06-01

An algorithm for the generation of non-uniform, locally orthogonal staggered unstructured spheroidal grids is described. This technique is designed to generate very high-quality staggered Voronoi-Delaunay meshes appropriate for general circulation modelling on the sphere, including applications to atmospheric simulation, ocean-modelling and numerical weather prediction. Using a recently developed Frontal-Delaunay refinement technique, a method for the construction of high-quality unstructured spheroidal Delaunay triangulations is introduced. A locally orthogonal polygonal grid, derived from the associated Voronoi diagram, is computed as the staggered dual. It is shown that use of the Frontal-Delaunay refinement technique allows for the generation of very high-quality unstructured triangulations, satisfying a priori bounds on element size and shape. Grid quality is further improved through the application of hill-climbing-type optimisation techniques. Overall, the algorithm is shown to produce grids with very high element quality and smooth grading characteristics, while imposing relatively low computational expense. A selection of uniform and non-uniform spheroidal grids appropriate for high-resolution, multi-scale general circulation modelling are presented. These grids are shown to satisfy the geometric constraints associated with contemporary unstructured C-grid-type finite-volume models, including the Model for Prediction Across Scales (MPAS-O). The use of user-defined mesh-spacing functions to generate smoothly graded, non-uniform grids for multi-resolution-type studies is discussed in detail.

The CARMENES search for exoplanets around M dwarfs. High-resolution optical and near-infrared spectroscopy of 324 survey stars

NASA Astrophysics Data System (ADS)

Reiners, A.; Zechmeister, M.; Caballero, J. A.; Ribas, I.; Morales, J. C.; Jeffers, S. V.; Schöfer, P.; Tal-Or, L.; Quirrenbach, A.; Amado, P. J.; Kaminski, A.; Seifert, W.; Abril, M.; Aceituno, J.; Alonso-Floriano, F. J.; Ammler-von Eiff, M.; Antona, R.; Anglada-Escudé, G.; Anwand-Heerwart, H.; Arroyo-Torres, B.; Azzaro, M.; Baroch, D.; Barrado, D.; Bauer, F. F.; Becerril, S.; Béjar, V. J. S.; Benítez, D.; Berdinas˜, Z. M.; Bergond, G.; Blümcke, M.; Brinkmöller, M.; del Burgo, C.; Cano, J.; Cárdenas Vázquez, M. C.; Casal, E.; Cifuentes, C.; Claret, A.; Colomé, J.; Cortés-Contreras, M.; Czesla, S.; Díez-Alonso, E.; Dreizler, S.; Feiz, C.; Fernández, M.; Ferro, I. M.; Fuhrmeister, B.; Galadí-Enríquez, D.; Garcia-Piquer, A.; García Vargas, M. L.; Gesa, L.; Galera, V. Gómez; González Hernández, J. I.; González-Peinado, R.; Grözinger, U.; Grohnert, S.; Guàrdia, J.; Guenther, E. W.; Guijarro, A.; Guindos, E. de; Gutiérrez-Soto, J.; Hagen, H.-J.; Hatzes, A. P.; Hauschildt, P. H.; Hedrosa, R. P.; Helmling, J.; Henning, Th.; Hermelo, I.; Hernández Arabí, R.; Hernández Castaño, L.; Hernández Hernando, F.; Herrero, E.; Huber, A.; Huke, P.; Johnson, E. N.; Juan, E. de; Kim, M.; Klein, R.; Klüter, J.; Klutsch, A.; Kürster, M.; Lafarga, M.; Lamert, A.; Lampón, M.; Lara, L. M.; Laun, W.; Lemke, U.; Lenzen, R.; Launhardt, R.; López del Fresno, M.; López-González, J.; López-Puertas, M.; López Salas, J. F.; López-Santiago, J.; Luque, R.; Magán Madinabeitia, H.; Mall, U.; Mancini, L.; Mandel, H.; Marfil, E.; Marín Molina, J. A.; Maroto Fernández, D.; Martín, E. L.; Martín-Ruiz, S.; Marvin, C. J.; Mathar, R. J.; Mirabet, E.; Montes, D.; Moreno-Raya, M. E.; Moya, A.; Mundt, R.; Nagel, E.; Naranjo, V.; Nortmann, L.; Nowak, G.; Ofir, A.; Oreiro, R.; Pallé, E.; Panduro, J.; Pascual, J.; Passegger, V. M.; Pavlov, A.; Pedraz, S.; Pérez-Calpena, A.; Medialdea, D. Pérez; Perger, M.; Perryman, M. A. C.; Pluto, M.; Rabaza, O.; Ramón, A.; Rebolo, R.; Redondo, P.; Reffert, S.; Reinhart, S.; Rhode, P.; Rix, H.-W.; Rodler, F.; Rodríguez, E.; Rodríguez-López, C.; Rodríguez Trinidad, A.; Rohloff, R.-R.; Rosich, A.; Sadegi, S.; Sánchez-Blanco, E.; Sánchez Carrasco, M. A.; Sánchez-López, A.; Sanz-Forcada, J.; Sarkis, P.; Sarmiento, L. F.; Schäfer, S.; Schmitt, J. H. M. M.; Schiller, J.; Schweitzer, A.; Solano, E.; Stahl, O.; Strachan, J. B. P.; Stürmer, J.; Suárez, J. C.; Tabernero, H. M.; Tala, M.; Trifonov, T.; Tulloch, S. M.; Ulbrich, R. G.; Veredas, G.; Vico Linares, J. I.; Vilardell, F.; Wagner, K.; Winkler, J.; Wolthoff, V.; Xu, W.; Yan, F.; Zapatero Osorio, M. R.

2018-04-01

The CARMENES radial velocity (RV) survey is observing 324 M dwarfs to search for any orbiting planets. In this paper, we present the survey sample by publishing one CARMENES spectrum for each M dwarf. These spectra cover the wavelength range 520-1710 nm at a resolution of at least R >80 000, and we measure its RV, Hα emission, and projected rotation velocity. We present an atlas of high-resolution M-dwarf spectra and compare the spectra to atmospheric models. To quantify the RV precision that can be achieved in low-mass stars over the CARMENES wavelength range, we analyze our empirical information on the RV precision from more than 6500 observations. We compare our high-resolution M-dwarf spectra to atmospheric models where we determine the spectroscopic RV information content, Q, and signal-to-noise ratio. We find that for all M-type dwarfs, the highest RV precision can be reached in the wavelength range 700-900 nm. Observations at longer wavelengths are equally precise only at the very latest spectral types (M8 and M9). We demonstrate that in this spectroscopic range, the large amount of absorption features compensates for the intrinsic faintness of an M7 star. To reach an RV precision of 1 m s-1 in very low mass M dwarfs at longer wavelengths likely requires the use of a 10 m class telescope. For spectral types M6 and earlier, the combination of a red visual and a near-infrared spectrograph is ideal to search for low-mass planets and to distinguish between planets and stellar variability. At a 4 m class telescope, an instrument like CARMENES has the potential to push the RV precision well below the typical jitter level of 3-4 m s-1.

Chiral-phase high-performance liquid chromatography of rotenoid racemates

USGS Publications Warehouse

Abidi, S.L.

1987-01-01

The high-performance liquid chromatograhic (HPLC) behavior of parent rotenoids (type I) and the hydroxyl-analogues (type II) on three different chiral stationary phases (CSPs) was studied. Separations of optical isomers were achieved in various degrees depending largely upon the rotenoidal structures and the CSP types employed. Enantiomers of all but elliptone compounds were separable on β-cyclodextrin-bonded silica (CDS). Without exception, the 12a-hydroxyrotenoid antipodes were resolved on Pirkle's phenylglycine-bonded silica (PGS) despite unsuccessful attenmpts to resolve the type I rotenoidal racemates. Conversely, optical resolution of the latter rotenoids was accomplished by using a helical polytriphenylmethylacrylate-coated silica (TPS) column and the observed separation factors (α values) ranged from 1.14 to 1.90. The results from HPLC of type II rotenoids on TPS (α = 1.00–1.63) suggested that variations in E-ring structures had profound influence on the resolution outcome. Conjugated double bonds on the E-ring and the desisopropylation of the five-membered E-ring ot type II rotenoids appeared to be important structural features for chiral recognition involving the TPS substrate. In both reversed-phase (CDS) and normal-pahse (PGS and TPS) HPLC modes, the less polar enantiomers were the 6aβ,12aβ-rotenoids as observed in most cases, though this relationship was reversed in the cases of deguelin and hydroxyelliptone probably due to conformational effects of rotenoidal ring systems.

Spectra of polarized thermal radiation in a cloudy atmosphere: Line-by-Line and Monte Carlo model for passive remote sensing of cirrus and polar clouds

NASA Astrophysics Data System (ADS)

Fomin, Boris; Falaleeva, Victoria

2016-07-01

A polarized high-resolution 1-D model has been presented for TIR (Thermal Infrared) remote sensing application. It is based on the original versions of MC (Monte Carlo) and LbL (Line-by-Line) algorithms, which have shown their effectiveness when modelling the thermal radiation atmospheric transfer, taking into account, the semi-transparent Ci-type and polar clouds scattering, as well as the direct consideration of the spectra of molecular absorption. This model may be useful in the planning of satellite experiments and in the validation of similar models, which use the "k-distribution" or other approximations, to account for gaseous absorption. The example simulations demonstrate that, the selective gas absorption does not only significantly affect the absorption and emission of radiation, but also, its polarization in the Ci-type clouds. As a result, the spectra of polarized radiation contain important information about the clouds, and а high-resolution polarized limb sounding in the TIR, seems to be a useful tool in obtaining information on cloud types and their vertical structures.

Novel autosomal dominant mandibulofacial dysostosis with ptosis: clinical description and exclusion of TCOF1

PubMed Central

Hedera, P; Toriello, H; Petty, E

2002-01-01

Methods: Six affected family members underwent a complete medical genetics physical examination and two affected subjects had skeletal survey. All available medical records were reviewed. Linkage analysis using the markers spanning the TCOF1 locus was performed. One typically affected family member had a high resolution karyotype. Results: Affected subjects had significant craniofacial abnormalities without any significant acral changes and thus had a phenotype consistent with a MFD variant. Distinctive features included hypoplasia of the zygomatic complex, micrognathia with malocclusion, auricular abnormalities with conductive hearing loss, and ptosis. Significantly negative two point lod scores were obtained for markers spanning the TCOF1 locus, excluding the possibility that the disease in our kindred is allelic with TCS. High resolution karyotype was normal. Conclusions: We report a kindred with a novel type of MFD that is not linked to the TCOF1 locus and is also clinically distinct from other types of AD MFD. Identification of additional families will facilitate identification of the gene causing this type of AD MFD and further characterisation of the clinical phenotype. PMID:12114479

A multiscale Bayesian data integration approach for mapping air dose rates around the Fukushima Daiichi Nuclear Power Plant.

PubMed

Wainwright, Haruko M; Seki, Akiyuki; Chen, Jinsong; Saito, Kimiaki

2017-02-01

This paper presents a multiscale data integration method to estimate the spatial distribution of air dose rates in the regional scale around the Fukushima Daiichi Nuclear Power Plant. We integrate various types of datasets, such as ground-based walk and car surveys, and airborne surveys, all of which have different scales, resolutions, spatial coverage, and accuracy. This method is based on geostatistics to represent spatial heterogeneous structures, and also on Bayesian hierarchical models to integrate multiscale, multi-type datasets in a consistent manner. The Bayesian method allows us to quantify the uncertainty in the estimates, and to provide the confidence intervals that are critical for robust decision-making. Although this approach is primarily data-driven, it has great flexibility to include mechanistic models for representing radiation transport or other complex correlations. We demonstrate our approach using three types of datasets collected at the same time over Fukushima City in Japan: (1) coarse-resolution airborne surveys covering the entire area, (2) car surveys along major roads, and (3) walk surveys in multiple neighborhoods. Results show that the method can successfully integrate three types of datasets and create an integrated map (including the confidence intervals) of air dose rates over the domain in high resolution. Moreover, this study provides us with various insights into the characteristics of each dataset, as well as radiocaesium distribution. In particular, the urban areas show high heterogeneity in the contaminant distribution due to human activities as well as large discrepancy among different surveys due to such heterogeneity. Copyright © 2016 Elsevier Ltd. All rights reserved.

Delineating Tree Types in a Complex Tropical Forest Setting Using High Resolution Multispectral Satellite Imagery

NASA Astrophysics Data System (ADS)

Cross, M.

2016-12-01

An improved process for the identification of tree types from satellite imagery for tropical forests is needed for more accurate assessments of the impact of forests on the global climate. La Selva Biological Station in Costa Rica was the tropical forest area selected for this particular study. WorldView-3 imagery was utilized because of its high spatial, spectral and radiometric resolution, its availability, and its potential to differentiate species in a complex forest setting. The first-step was to establish confidence in the high spatial and high radiometric resolution imagery from WorldView-3 in delineating tree types within a complex forest setting. In achieving this goal, ASD field spectrometer data were collected of specific tree species to establish solid ground control within the study site. The spectrometer data were collected from the top of each specific tree canopy utilizing established towers located at La Selva Biological Station so as to match the near-nadir view of the WorldView-3 imagery. The ASD data was processed utilizing the spectral response functions for each of the WorldView-3 bands to convert the ASD data into a band specific reflectivity. This allowed direct comparison of the ASD spectrometer reflectance data to the WorldView-3 multispectral imagery. The WorldView-3 imagery was processed to surface reflectance using two standard atmospheric correction procedures and the proprietary DigitalGlobe Atmospheric Compensation (AComp) product. The most accurate correction process was identified through comparison to the spectrometer data collected. A series of statistical measures were then utilized to access the accuracy of the processed imagery and which imagery bands are best suited for tree type identification. From this analysis, a segmentation/classification process was performed to identify individual tree type locations within the study area. It is envisioned the results of this study will improve traditional forest classification processes, provide more accurate assessments of species density and distribution, facilitate a more accurate biomass estimate of the tropical forest which will impact the accuracy of tree carbon storage estimates, and ultimately assist in developing a better overall characterization of tropical rainforest dynamics.

The High Angular Resolution Multiplicity of Massive Stars

DTIC Science & Technology

2009-02-01

binaries: visual – stars: early-type – stars: individual ( iota Ori, delta Ori, delta Sco) – techniques: interferometric Online-only material...STATEMENT Approved for public release; distribution unlimited 13. SUPPLEMENTARY NOTES 14. ABSTRACT 15. SUBJECT TERMS 16. SECURITY

Hybrid-mode read-in integrated circuit for infrared scene projectors

NASA Astrophysics Data System (ADS)

Cho, Min Ji; Shin, Uisub; Lee, Hee Chul

2017-05-01

The infrared scene projector (IRSP) is a tool for evaluating infrared sensors by producing infrared images. Because sensor testing with IRSPs is safer than field testing, the usefulness of IRSPs is widely recognized at present. The important performance characteristics of IRSPs are the thermal resolution and the thermal dynamic range. However, due to an existing trade-off between these requirements, it is often difficult to find a workable balance between them. The conventional read-in integrated circuit (RIIC) can be classified into two types: voltage-mode and current-mode types. An IR emitter driven by a voltage-mode RIIC offers a fine thermal resolution. On the other hand, an emitter driven by the current-mode RIIC has the advantage of a wide thermal dynamic range. In order to provide various scenes, i.e., from highresolution scenes to high-temperature scenes, both of the aforementioned advantages are required. In this paper, a hybridmode RIIC which is selectively operated in two modes is proposed. The mode-selective characteristic of the proposed RIIC allows users to generate high-fidelity scenes regardless of the scene content. A prototype of the hybrid-mode RIIC was fabricated using a 0.18-μm 1-poly 6-metal CMOS process. The thermal range and the thermal resolution of the IR emitter driven by the proposed circuit were calculated based on measured data. The estimated thermal dynamic range of the current mode was from 261K to 790K, and the estimated thermal resolution of the voltage mode at 300K was 23 mK with a 12-bit gray-scale resolution.

Correlation and agreement between eplet mismatches calculated using serological, low-intermediate and high resolution molecular human leukocyte antigen typing methods

PubMed Central

Fidler, Samantha; D’Orsogna, Lloyd; Irish, Ashley B.; Lewis, Joshua R.; Wong, Germaine; Lim, Wai H.

2018-01-01

Structural human leukocyte antigen (HLA) matching at the eplet level can be identified by HLAMatchmaker, which requires the entry of four-digit alleles. The aim of this study was to evaluate the agreement between eplet mismatches calculated by serological and two-digit typing methods compared to high-resolution four-digit typing. In a cohort of 264 donor/recipient pairs, the evaluation of measurement error was assessed using intra-class correlation to confirm the absolute agreement between the number of eplet mismatches at class I (HLA-A, -B, C) and II loci (HLA-DQ and -DR) calculated using serological or two-digit molecular typing compared to four-digit molecular typing methods. The proportion of donor/recipient pairs with a difference of >5 eplet mismatches between the HLA typing methods was also determined. Intra-class correlation coefficients between serological and four-digit molecular typing methods were 0.969 (95% confidence intervals [95% CI] 0.960–0.975) and 0.926 (95% CI 0.899–0.944), respectively; and 0.995 (95% CI 0.994–0.996) and 0.993 (95% CI 0.991–0.995), respectively between two-digit and four-digit molecular typing methods. The proportion of donor/recipient pairs with a difference of >5 eplet mismatches at class I and II loci was 4% and 16% for serological versus four-digit molecular typing methods, and 0% and 2% for two-digit versus four-digit molecular typing methods, respectively. In this small predominantly Caucasian population, compared with serology, there is a high level of agreement in the number of eplet mismatches calculated using two-compared to four-digit molecular HLA-typing methods, suggesting that two-digit typing may be sufficient in determining eplet mismatch load in kidney transplantation. PMID:29568344

New high-resolution electrostatic ion mass analyzer using time of flight

NASA Technical Reports Server (NTRS)

Hamilton, D. C.; Gloeckler, G.; Ipavich, F. M.; Lundgren, R. A.; Sheldon, R. B.

1990-01-01

The design of a high-resolution ion-mass analyzer is described, which is based on an accurate measurement of the time of flight (TOF) of ions within a region configured to produce a harmonic potential. In this device, the TOF, which is independent of ion energy, is determined from a start pulse from secondary electrons produced when the ion passes through a thin carbon foil at the entrance of the TOF region and at a stop pulse from the ion striking a microchannel plate upon exciting the region. A laboratory prototype instrument called 'VMASS' was built and was tested at the Goddard Space Flight Center electrostatic accelerator, showing a good mass resolution of the instrument. Sensors of the VMASS type will form part of the WIND Solar Wind and Suprathermal Ion experiment, the Soho mission, and the Advanced Composition Explorer.

High resolution MRI of the normal finger at 0.1 T: anatomic correlations.

PubMed

Drapé, J L; Constantinesco, A; Arbogast, S; Sick, H; Wolfram-Gabel, R; Brunot, B

1992-01-01

MR images of the fingers are obtained in a 128 x 128 or 256 x 256 matrix format using a prototype of a mini imager dedicated to the hand. The vertical field of 0.1 T is provided by an electro-magnet with an air gap of 15 cm equipped with a single solenoidal coil. No Faraday cage is used. The maximum in plane pixel resolution of 100 mu is obtained for a field of view of 2.5 cm with a slice thickness of 2 mm. The identification of fine structures of the finger is demonstrated by the anatomical and histological correlations. This type of imager which is adapted to very limited field of views demonstrate that high resolution MRI of limb extremities can be achieved at 0.1 T.

VizieR Online Data Catalog: Orphan stream high-resolution spectroscopic study (Casey+, 2014)

NASA Astrophysics Data System (ADS)

Casey, A. R.; Keller, S. C.; da Costa, G.; Frebel, A.; Maunder, E.

2017-06-01

High-resolution spectra for five Orphan stream candidates and seven well-studied standard stars have been obtained with the Magellan Inamori Kyocera Echelle spectrograph (Bernstein et al. 2003SPIE.4841.1694B) on the Magellan Clay telescope. These objects were observed in 2011 March using a 1" wide slit in mean seeing of 0.9". This slit configuration provides continuous spectral coverage from 333 nm to 915 nm, with a spectral resolution of R=25000 in the blue arm and R=28000 in the red arm. A minimum of 10 exposures of each calibration type (biases, flat fields, and diffuse flats) were observed in the afternoon of each day, with additional flat-field and Th-Ar arc lamp exposures performed throughout the night to ensure an accurate wavelength calibration. (3 data files).

The use of colonic and anorectal high-resolution manometry and its place in clinical work and in research.

PubMed

Dinning, P G; Carrington, E V; Scott, S M

2015-12-01

In the esophagus, high-resolution manometry (HRM) has become a standard diagnostic tool in the investigation of suspected motility disorders. However, at the opposite end of the digestive tract (i.e., the colon and anorectum), the use of HRM still remains in its infancy, with relatively few published studies in the scientific literature. Further, the clinical utility of those studies that have been performed is largely undetermined. This review assesses all of the HRM studies published to date from both the colon and anorectum, explores the catheter types used, and attempts to determine the worth of HRM over traditional 'low-resolution' recordings from the same regions. Ultimately, this review addresses whether HRM currently provides information that will benefit patient diagnosis and treatment. © 2015 John Wiley & Sons Ltd.

High resolution modelling and observation of wind-driven surface currents in a semi-enclosed estuary

NASA Astrophysics Data System (ADS)

Nash, S.; Hartnett, M.; McKinstry, A.; Ragnoli, E.; Nagle, D.

2012-04-01

Hydrodynamic circulation in estuaries is primarily driven by tides, river inflows and surface winds. While tidal and river data can be quite easily obtained for input to hydrodynamic models, sourcing accurate surface wind data is problematic. Firstly, the wind data used in hydrodynamic models is usually measured on land and can be quite different in magnitude and direction from offshore winds. Secondly, surface winds are spatially-varying but due to a lack of data it is common practice to specify a non-varying wind speed and direction across the full extents of a model domain. These problems can lead to inaccuracies in the surface currents computed by three-dimensional hydrodynamic models. In the present research, a wind forecast model is coupled with a three-dimensional numerical model of Galway Bay, a semi-enclosed estuary on the west coast of Ireland, to investigate the effect of surface wind data resolution on model accuracy. High resolution and low resolution wind fields are specified to the model and the computed surface currents are compared with high resolution surface current measurements obtained from two high frequency SeaSonde-type Coastal Ocean Dynamics Applications Radars (CODAR). The wind forecast models used for the research are Harmonie cy361.3, running on 2.5 and 0.5km spatial grids for the low resolution and high resolution models respectively. The low-resolution model runs over an Irish domain on 540x500 grid points with 60 vertical levels and a 60s timestep and is driven by ECMWF boundary conditions. The nested high-resolution model uses 300x300 grid points on 60 vertical levels and a 12s timestep. EFDC (Environmental Fluid Dynamics Code) is used for the hydrodynamic model. The Galway Bay model has ten vertical layers and is resolved spatially and temporally at 150m and 4 sec respectively. The hydrodynamic model is run for selected hindcast dates when wind fields were highly energetic. Spatially- and temporally-varying wind data is provided by offline coupling with the wind forecast models. Modelled surface currents show good correlation with CODAR observed currents and the resolution of the surface wind data is shown to be important for model accuracy.

Optical design of a Michelson wide-field multiple-aperture telescope

NASA Astrophysics Data System (ADS)

Cassaing, Frederic; Sorrente, Beatrice; Fleury, Bruno; Laubier, David

2004-02-01

Multiple-Aperture Optical Telescopes (MAOTs) are a promising solution for very high resolution imaging. In the Michelson configuration, the instrument is made of sub-telescopes distributed in the pupil and combined by a common telescope via folding periscopes. The phasing conditions of the sub-pupils lead to specific optical constraints in these subsystems. The amplitude of main contributors to the wavefront error (WFE) is given as a function of high level requirements (such as field or resolution) and free parameters, mainly the sub-telescope type, magnification and diameter. It is shown that for the periscopes, the field-to-resolution ratio is the main design driver and can lead to severe specifications. The effect of sub-telescopes aberrations on the global WFE can be minimized by reducing their diameter. An analytical tool for the MAOT design has been derived from this analysis, illustrated and validated in three different cases: LEO or GEO Earth observation and astronomy with extremely large telescopes. The last two cases show that a field larger than 10 000 resolution elements can be covered with a very simple MAOT based on Mersenne paraboloid-paraboloid sub-telescopes. Michelson MAOTs are thus a solution to be considered for high resolution wide-field imaging, from space or ground.

Applications using high-Tc superconducting terahertz emitters

PubMed Central

Nakade, Kurama; Kashiwagi, Takanari; Saiwai, Yoshihiko; Minami, Hidetoshi; Yamamoto, Takashi; Klemm, Richard A.; Kadowaki, Kazuo

2016-01-01

Using recently-developed THz emitters constructed from single crystals of the high-Tc superconductor Bi2Sr2CaCu2O8+δ, we performed three prototype tests of the devices to demonstrate their unique characteristic properties for various practical applications. The first is a compact and simple transmission type of THz imaging system using a Stirling cryocooler. The second is a high-resolution Michelson interferometer used as a phase-sensitive reflection-type imaging system. The third is a system with precise temperature control to measure the liquid absorption coefficient. The detailed characteristics of these systems are discussed. PMID:26983905

Compact type-I coil planet centrifuge for counter-current chromatography

PubMed Central

Yang, Yi; Gu, Dongyu; Liu, Yongqiang; Aisa, Haji Akber; Ito, Yoichiro

2009-01-01

A compact type-I coil planet centrifuge has been developed for performing counter-current chromatography. It has a revolution radius of 10 cm and a column holder height of 5 cm compared with 37 cm and 50 cm in the original prototype, respectively. The reduction in the revolution radius and column length permits application of higher revolution speed and more stable balancing of the rotor which leads us to learn more about its performance and the future potential of type-I coil planet centrifuge. The chromatographic performance of this apparatus was evaluated in terms of retention of the stationary phase (Sf), peak resolution (Rs), theoretical plate (N) and peak retention time (tR). The results of the experiment indicated that increasing the revolution speed slightly improved both the retention of the stationary phase and the peak resolution while the separation time is remarkably shortened to yield an excellent peak resolution at a revolution speed of 800 rpm. With a 12 ml capacity coiled column, DNP-glu, DNP-β-ala and DNP-ala were resolved at Rs of 2.75 and 2.16 within 90 min at a flow rate of 0.4 ml/min. We believe that the compact type-I coil planet centrifuge has a high analytical potential. PMID:20060979

Compact type-I coil planet centrifuge for counter-current chromatography.

PubMed

Yang, Yi; Gu, Dongyu; Liu, Yongqiang; Aisa, Haji Akber; Ito, Yoichiro

2010-02-19

A compact type-I coil planet centrifuge has been developed for performing counter-current chromatography. It has a revolution radius of 10 cm and a column holder height of 5 cm compared with 37 and 50 cm in the original prototype, respectively. The reduction in the revolution radius and column length permits application of higher revolution speed and more stable balancing of the rotor which leads us to learn more about its performance and the future potential of type-I coil planet centrifuge. The chromatographic performance of this apparatus was evaluated in terms of retention of the stationary phase (S(f)), peak resolution (R(s)), theoretical plate (N) and peak retention time (t(R)). The results of the experiment indicated that increasing the revolution speed slightly improved both the retention of the stationary phase and the peak resolution while the separation time is remarkably shortened to yield an excellent peak resolution at a revolution speed of 800 rpm. With a 12 ml capacity coiled column, DNP-DL-glu, DNP-beta-ala and DNP-l-ala were resolved at R(s) of 2.75 and 2.16 within 90 min at a flow rate of 0.4 ml/min. We believe that the compact type-I coil planet centrifuge has a high analytical potential. Published by Elsevier B.V.

Infrared near-field spectroscopy of trace explosives using an external cavity quantum cascade laser.

PubMed

Craig, Ian M; Taubman, Matthew S; Lea, A Scott; Phillips, Mark C; Josberger, Erik E; Raschke, Markus B

2013-12-16

Utilizing a broadly-tunable external cavity quantum cascade laser for scattering-type scanning near-field optical microscopy (s-SNOM), we measure infrared spectra of particles of explosives by probing characteristic nitro-group resonances in the 7.1-7.9 µm wavelength range. Measurements are presented with spectral resolution of 0.25 cm(-1), spatial resolution of 25 nm, sensitivity better than 100 attomoles, and at a rapid acquisition time of 90 s per spectrum. We demonstrate high reproducibility of the acquired s-SNOM spectra with very high signal-to-noise ratios and relative noise of <0.02 in self-homodyne detection.

Massive stars in the Sagittarius Dwarf Irregular Galaxy

NASA Astrophysics Data System (ADS)

Garcia, Miriam

2018-02-01

Low metallicity massive stars hold the key to interpret numerous processes in the past Universe including re-ionization, starburst galaxies, high-redshift supernovae, and γ-ray bursts. The Sagittarius Dwarf Irregular Galaxy [SagDIG, 12+log(O/H) = 7.37] represents an important landmark in the quest for analogues accessible with 10-m class telescopes. This Letter presents low-resolution spectroscopy executed with the Gran Telescopio Canarias that confirms that SagDIG hosts massive stars. The observations unveiled three OBA-type stars and one red supergiant candidate. Pending confirmation from high-resolution follow-up studies, these could be the most metal-poor massive stars of the Local Group.

Multiscale sampling of plant diversity: Effects of minimum mapping unit size

USGS Publications Warehouse

Stohlgren, T.J.; Chong, G.W.; Kalkhan, M.A.; Schell, L.D.

1997-01-01

Only a small portion of any landscape can be sampled for vascular plant diversity because of constraints of cost (salaries, travel time between sites, etc.). Often, the investigator decides to reduce the cost of creating a vegetation map by increasing the minimum mapping unit (MMU), and/or by reducing the number of vegetation classes to be considered. Questions arise about what information is sacrificed when map resolution is decreased. We compared plant diversity patterns from vegetation maps made with 100-ha, 50-ha, 2-ha, and 0.02-ha MMUs in a 754-ha study area in Rocky Mountain National Park, Colorado, United States, using four 0.025-ha and 21 0.1-ha multiscale vegetation plots. We developed and tested species-log(area) curves, correcting the curves for within-vegetation type heterogeneity with Jaccard's coefficients. Total species richness in the study area was estimated from vegetation maps at each resolution (MMU), based on the corrected species-area curves, total area of the vegetation type, and species overlap among vegetation types. With the 0.02-ha MMU, six vegetation types were recovered, resulting in an estimated 552 species (95% CI = 520-583 species) in the 754-ha study area (330 plant species were observed in the 25 plots). With the 2-ha MMU, five vegetation types were recognized, resulting in an estimated 473 species for the study area. With the 50-ha MMU, 439 plant species were estimated for the four vegetation types recognized in the study area. With the 100-ha MMU, only three vegetation types were recognized, resulting in an estimated 341 plant species for the study area. Locally rare species and keystone ecosystems (areas of high or unique plant diversity) were missed at the 2-ha, 50-ha, and 100-ha scales. To evaluate the effects of minimum mapping unit size requires: (1) an initial stratification of homogeneous, heterogeneous, and rare habitat types; and (2) an evaluation of within-type and between-type heterogeneity generated by environmental gradients and other factors. We suggest that at least some portions of vegetation maps created at a coarser level of resolution be validated at a higher level of resolution.

Satellite Remote Sensing of Cropland Characteristics in 30m Resolution: The First North American Continental-Scale Classification on High Performance Computing Platforms

NASA Astrophysics Data System (ADS)

Massey, Richard

Cropland characteristics and accurate maps of their spatial distribution are required to develop strategies for global food security by continental-scale assessments and agricultural land use policies. North America is the major producer and exporter of coarse grains, wheat, and other crops. While cropland characteristics such as crop types are available at country-scales in North America, however, at continental-scale cropland products are lacking at fine sufficient resolution such as 30m. Additionally, applications of automated, open, and rapid methods to map cropland characteristics over large areas without the need of ground samples are needed on efficient high performance computing platforms for timely and long-term cropland monitoring. In this study, I developed novel, automated, and open methods to map cropland extent, crop intensity, and crop types in the North American continent using large remote sensing datasets on high-performance computing platforms. First, a novel method was developed in this study to fuse pixel-based classification of continental-scale Landsat data using Random Forest algorithm available on Google Earth Engine cloud computing platform with an object-based classification approach, recursive hierarchical segmentation (RHSeg) to map cropland extent at continental scale. Using the fusion method, a continental-scale cropland extent map for North America at 30m spatial resolution for the nominal year 2010 was produced. In this map, the total cropland area for North America was estimated at 275.2 million hectares (Mha). This map was assessed for accuracy using randomly distributed samples derived from United States Department of Agriculture (USDA) cropland data layer (CDL), Agriculture and Agri-Food Canada (AAFC) annual crop inventory (ACI), Servicio de Informacion Agroalimentaria y Pesquera (SIAP), Mexico's agricultural boundaries, and photo-interpretation of high-resolution imagery. The overall accuracies of the map are 93.4% with a producer's accuracy for crop class at 85.4% and user's accuracy of 74.5% across the continent. The sub-country statistics including state-wise and county-wise cropland statistics derived from this map compared well in regression models resulting in R2 > 0.84. Secondly, an automated phenological pattern matching (PPM) method to efficiently map cropping intensity was also developed in this study. This study presents a continental-scale cropping intensity map for the North American continent at 250m spatial resolution for 2010. In this map, the total areas for single crop, double crop, continuous crop, and fallow were estimated to be 123.5 Mha, 11.1 Mha, 64.0 Mha, and 83.4 Mha, respectively. This map was assessed using limited country-level reference datasets derived from United States Department of Agriculture cropland data layer and Agriculture and Agri-Food Canada annual crop inventory with overall accuracies of 79.8% and 80.2%, respectively. Third, two novel and automated decision tree classification approaches to map crop types across the conterminous United States (U.S.) using MODIS 250 m resolution data: 1) generalized, and 2) year-specific classification were developed. The classification approaches use similarities and dissimilarities in crop type phenology derived from NDVI time-series data for the two approaches. Annual crop type maps were produced for 8 major crop types in the United States using the generalized classification approach for 2001-2014 and the year-specific approach for 2008, 2010, 2011 and 2012. The year-specific classification had overall accuracies greater than 78%, while the generalized classifier had accuracies greater than 75% for the conterminous U.S. for 2008, 2010, 2011, and 2012. The generalized classifier enables automated and routine crop type mapping without repeated and expensive ground sample collection year after year with overall accuracies > 70% across all independent years. Taken together, these cropland products of extent, cropping intensity, and crop types, are significantly beneficial in agricultural and water use planning and monitoring to formulate policies towards global and North American food security issues.

A High Space-Time Resolution Dataset Linking Meteorological Forcing and Hydro-Sedimentary Response in a Mesoscale Mediterranean Catchment (Auzon) of the Ardèche Region, France

NASA Astrophysics Data System (ADS)

Nord, G.; Braud, I.; Boudevillain, B.; Gérard, S.; Molinié, G.; Vandervaere, J. P.; Huza, J.; Le Coz, J.; Dramais, G.; Legout, C.; Berne, A.; Grazioli, J.; Raupach, T.; Van Baelen, J.; Wijbrans, A.; Delrieu, G.; Andrieu, J.; Caliano, M.; Aubert, C.; Teuling, R.; Le Boursicaud, R.; Branger, F.; Vincendon, B.; Horner, I.

2014-12-01

A comprehensive hydrometeorological dataset is presented spanning the period 1 Jan 2011-31 Dec 2014 to improve the understanding and simulation of the hydrological processes leading to flash floods in a mesoscale catchment (Auzon, 116 km2) of the Mediterranean region. The specificity of the dataset is its high space-time resolution, especially concerning rainfall and the hydrological response which is particularly adapted to the highly spatially variable rainfall events that may occur in this region. This type of dataset is rare in scientific literature because of the quantity and type of sensors for meteorology and surface hydrology. Rainfall data include continuous precipitation measured by rain-gages (5 min time step for the research network of 21 rain-gages and 1h time step for the operational network of 9 rain-gages), S-band Doppler dual-polarization radar (1 km2, 5 min resolution), and disdrometers (11 sensors working at 1 min time step). During the special observation period (SOP-1) and enhanced observation period (Sep-Dec 2012, Sep-Dec 2013) of the HyMeX (Hydrological Cycle in the Mediterranean Experiment) project, two X-band radars provided precipitation measurements at very fine spatial and temporal scales (1 ha, 5 min). Meteorological data are taken from the operational surface weather observation stations of Meteo France at the hourly time resolution (6 stations in the region of interest). The monitoring of surface hydrology and suspended sediment is multi-scale and based on nested catchments. Three hydrometric stations measure water discharge and additional physico-chemical variables at a 2-10 min time resolution. Two experimental plots monitor overland flow and erosion at 1 min time resolution on a hillslope with vineyard. A network of 11 gauges continuously measures water level and temperature in headwater subcatchments at a time resolution of 2-5 min. A network of soil moisture sensors enable the continuous measurement of soil volumetric water content at 20 min time resolution at 9 sites. Additionally, opportunistic observations (soil moisture measurements and stream gauging) were performed during floods between 2012 and 2014. The data are appropriate for understanding rainfall variability, improving areal rainfall estimations and progress in distributed hydrological modelling.

A High Space-Time Resolution Dataset Linking Meteorological Forcing and Hydro-Sedimentary Response in a Mesoscale Mediterranean Catchment (Auzon) of the Ardèche Region, France

NASA Astrophysics Data System (ADS)

Nord, G.; Braud, I.; Boudevillain, B.; Gérard, S.; Molinié, G.; Vandervaere, J. P.; Huza, J.; Le Coz, J.; Dramais, G.; Legout, C.; Berne, A.; Grazioli, J.; Raupach, T.; Van Baelen, J.; Wijbrans, A.; Delrieu, G.; Andrieu, J.; Caliano, M.; Aubert, C.; Teuling, R.; Le Boursicaud, R.; Branger, F.; Vincendon, B.; Horner, I.

2015-12-01

A comprehensive hydrometeorological dataset is presented spanning the period 1 Jan 2011-31 Dec 2014 to improve the understanding and simulation of the hydrological processes leading to flash floods in a mesoscale catchment (Auzon, 116 km2) of the Mediterranean region. The specificity of the dataset is its high space-time resolution, especially concerning rainfall and the hydrological response which is particularly adapted to the highly spatially variable rainfall events that may occur in this region. This type of dataset is rare in scientific literature because of the quantity and type of sensors for meteorology and surface hydrology. Rainfall data include continuous precipitation measured by rain-gages (5 min time step for the research network of 21 rain-gages and 1h time step for the operational network of 9 rain-gages), S-band Doppler dual-polarization radar (1 km2, 5 min resolution), and disdrometers (11 sensors working at 1 min time step). During the special observation period (SOP-1) and enhanced observation period (Sep-Dec 2012, Sep-Dec 2013) of the HyMeX (Hydrological Cycle in the Mediterranean Experiment) project, two X-band radars provided precipitation measurements at very fine spatial and temporal scales (1 ha, 5 min). Meteorological data are taken from the operational surface weather observation stations of Meteo France at the hourly time resolution (6 stations in the region of interest). The monitoring of surface hydrology and suspended sediment is multi-scale and based on nested catchments. Three hydrometric stations measure water discharge and additional physico-chemical variables at a 2-10 min time resolution. Two experimental plots monitor overland flow and erosion at 1 min time resolution on a hillslope with vineyard. A network of 11 gauges continuously measures water level and temperature in headwater subcatchments at a time resolution of 2-5 min. A network of soil moisture sensors enable the continuous measurement of soil volumetric water content at 20 min time resolution at 9 sites. Additionally, opportunistic observations (soil moisture measurements and stream gauging) were performed during floods between 2012 and 2014. The data are appropriate for understanding rainfall variability, improving areal rainfall estimations and progress in distributed hydrological modelling.

Abstract ID: 242 Simulation of a Fast Timing Micro-Pattern Gaseous Detector for TOF-PET.

PubMed

Radogna, Raffaella; Verwilligen, Piet

2018-01-01

Micro-Pattern Gas Detectors (MPGDs) are a new generation of gaseous detectors that have been developed thanks to advances in micro-structure technology. The main features of the MPGDs are: high rate capability (>50 MHz/cm 2 ); excellent spatial resolution (down to 50 μm); good time resolution (down to 3 ns); reduced radiation length, affordable costs, and possible flexible geometries. A new detector layout has been recently proposed that aims at combining both the high spatial resolution and high rate capability (100 MHz/cm 2 ) of the current state-of-the-art MPGDs with a high time resolution. This new type of MPGD is named the Fast Timing MPGD (FTM) detector [1,2]. The FTM developed for detecting charged particles can potentially reach sub-millimeter spatial resolution and 100 ps time resolution. This contribution introduces a Fast Timing MPGD technology optimized to detect photons, as an innovative PET imaging detector concept and emphases the importance of full detector simulation to guide the design of the detector geometry. The design and development of a new FTM, combining excellent time and spatial resolution, while exploiting the advantages of a reasonable energy resolution, will be a boost for the design of affordable TOF-PET scanner with improved image contrast. The use of such an affordable gas detector allows to instrument large areas in a cost-effective way, and to increase in image contrast for shorter scanning times (lowering the risk for the patient) and better diagnosis of the disease. In this report a dedicated simulation study is performed to optimize the detector design in the contest of the INFN project MPGD-Fatima. Results are obtained with ANSYS, COMSOL, GARFIELD++ and GEANT4 simulation tools. The final detector layout will be trade-off between fast time and good energy resolution. Copyright © 2017.

Future prospects for high resolution X-ray spectrometers

NASA Technical Reports Server (NTRS)

Canizares, C. R.

1981-01-01

Capabilities of the X-ray spectroscopy payloads were compared. Comparison of capabilities of AXAF in the context of the science to be achieved is reported. The Einstein demonstrated the tremendous scientific power of spectroscopy to probe deeply the astrophysics of all types of celestial X-ray source. However, it has limitations in sensitivity and resolution. Each of the straw man instruments has a sensitivity that is at least an order of magnitude better than that of the Einstein FPSC. The AXAF promises powerful spectral capability.

High-resolution Rainfall Mapping in Dallas-Fort Worth (DFW) Urban Network of Radars at Multiple Frequencies

NASA Astrophysics Data System (ADS)

Chandra, Chandrasekar V.; Chen*, Haonan

2015-04-01

Urban flash flood is one of the most commonly encountered hazardous weather phenomena. Unfortunately, the rapid urbanization has made the densely populated areas even more vulnerable to flood risks. Hence, accurate and timely monitoring of rainfall at high spatiotemporal resolution is critical to severe weather warning and civil defense, especially in urban areas. However, it is still challenging to produce high-resolution products based on the large S-band National Weather Service (NWS) Next-Generation Weather Radar (NEXRAD), due to the sampling limitations and Earth curvature effect. Since 2012, the U.S. National Science Foundation Engineering Research Center (NSF-ERC) for Collaborative Adaptive Sensing of the Atmosphere (CASA) has initiated the development of Dallas-Fort Worth (DFW) radar remote sensing network for urban weather hazards mitigation. The DFW urban radar network consists of a combination of high-resolution X-band radars and a standard NWS NEXRAD radar operating at S-band frequency. High-resolution quantitative precipitation estimation (QPE) is one of the major research goals in the deployment of this urban radar network. It has been shown in the literature that the dual-polarization radar techniques can improve the QPE accuracy over traditional single-polarization radars by rendering more measurements to enhance the data quality, providing more information about rain drop size distribution (DSD), and implying more characteristics of different hydrometeor types. This paper will present the real-time dual-polarization CASA DFW QPE system, which is developed via fusion of observations from both the high-resolution X band radar network and the S-band NWS radar. The specific dual-polarization rainfall algorithms at different frequencies (i.e., S- and X-band) will be described in details. In addition, the fusion methodology combining observations at different temporal resolution will be presented. In order to demonstrate the capability of rainfall estimation of the CASA DFW QPE system, rainfall measurements from ground rain gauges will be used for evaluation purposes. This high-resolution QPE system is used for urban flash flood forecasting when coupled with hydrological models.

Measurement of magnetic field aligned potential differences using high resolution conjugate photoelectron energy spectra

NASA Technical Reports Server (NTRS)

Peterson, W. K.; Doering, J. P.; Potemra, T. A.; Bostrom, C. O.; Brace, L. H.; Heelis, R. A.; Hanson, W. B.

1977-01-01

Simultaneous high-resolution observations of a distinctive feature in the energy spectrum of conjugate photoelectrons and spacecraft potential relative to the local ionosphere have allowed the net potential difference between magnetic conjugate points at latitudes below the region of low-energy (i.e., lower than 100 eV) auroral electron precipitation to be determined. Measurements made at 300 km from Atmosphere Explorer C show that there is normally no net potential difference between hemispheres in this region, which extended up to invariant latitudes as high as 74 deg. Two types of apparently related anomalous behavior were infrequently observed at high latitudes. During these periods the incident flux of conjugate photoelectrons was either decelerated by about 3 eV or was not detected.

Therapeutic Outcome of Achalasia Based on High-Resolution Manometry: A Korean Multicenter Study.

PubMed

Lee, Hyuk; Chung, Hyunsoo; Lee, Tae Hee; Hong, Kyoung Sup; Youn, Young Hoon; Park, Jung Ho; Park, Hyung Seok; Park, Hyojin

2017-09-11

Because achalasia subtype is associated with therapeutic response, it is possible that regional differences in subtype distribution could lead to differences in therapeutic outcomes. We aimed to evaluate and compare high-resolution manometry (HRM) profiles among the different subtypes of achalasia and to elucidate predictive factors associated with treatment outcomes. Patients who were diagnosed with achalasia using HRM at 4 Korean university hospitals were retrospectively identified and analyzed. Sixty-four patients with untreated achalasia were divided into 3 subtypes using the Chicago classification system. Clinical characteristics, manometric features, and treatment outcomes were compared. Among 64 patients diagnosed with achalasia, 31 patients were classified as type I, 27 as type II, and 6 as type III. Regarding HRM parameters, there were statistically significant differences in basal lower esophageal sphincter pressure, 4-second-integrated relaxation pressure, residual upper esophageal sphincter pressure, body amplitude, and maximal intrabolus pressure between subtypes. Regarding therapeutic outcome, type II patients (overall success rate of 80.0%) were more likely to respond than type I (55.2%) or type III (33.2%) patients. Multivariate analysis demonstrated that achalasia subtype (type I vs. III, P = 0.072; type II vs. III, P = 0.005), therapeutic modality (dilation vs. pharmacologic, P = 0.013; laparoscopic Heller's myotomy vs. pharmacologic, P = 0.006), and HRM-measured esophageal length (<27.5 vs. ≥27.5 cm, P = 0.014) are independent predictive factors for therapeutic failure. Patients with type II achalasia had better treatment outcomes than patients with other achalasia subtypes. Achalasia subtype, therapeutic modality, and esophageal length are independent predictive factors of therapeutic outcome.

Treatment of achalasia in the era of high-resolution manometry

PubMed Central

Torresan, Francesco; Ioannou, Alexandros; Azzaroli, Francesco; Bazzoli, Franco

2015-01-01

Esophageal achalasia is a primary motility disorder characterized by impaired lower esophageal sphincter relaxation and absence of esophageal peristalsis leading to impaired bolus transit, manifested with symptoms such as dysphagia, regurgitation, retrosternal pain, and weight loss. The standard diagnostic tool is esophageal manometry which demonstrates incomplete relaxation of the lower esophageal sphincter and impaired esophageal peristalsis. Recently, a new advanced technique, high-resolution manometry (HRM) with the addition of pressure topography plotting, using multiple sensors to capture the manometric data as a spatial continuum, allows a detailed pressure recording of the esophageal motility. This technique, currently the gold standard for the diagnosis of achalasia, has led to a subclassification of three manometric types that seem to have different responsiveness to treatment. Because its pathogenesis is as yet unknown, achalasia treatment options are not curative. Type II achalasia patients respond better to treatment compared to those with types I and III. Low-risk patients with type I or II achalasia have good outcome with both graded pneumatic dilatations and laparoscopic Heller myotomy, while type III achalasia patients respond better to laparoscopic Heller myotomy. Although, type III achalasia patients responds less in comparison to types I and II to laparoscopic Heller myotomy. Peroral endoscopic myotomy is a promising new technique but long-term follow-up studies for its safety and efficacy must be performed. This article reviews the current therapeutic options, highlighting the impact of HRM to predict the outcome and the new insights for the treatment of achalasia. PMID:26130022

High-Resolution Structure and Mechanism of an F/V-Hybrid Rotor Ring in a Na+-coupled ATP Synthase

PubMed Central

Matthies, Doreen; Zhou, Wenchang; Klyszejko, Adriana L.; Anselmi, Claudio; Yildiz, Özkan; Brandt, Karsten; Müller, Volker; Faraldo-Gómez, José D.; Meier, Thomas

2014-01-01

All rotary ATPases catalyze the interconversion of ATP and ADP-Pi through a mechanism that is coupled to the transmembrane flow of H+ or Na+. Physiologically, however, F/A-type enzymes specialize in ATP synthesis driven by downhill ion diffusion, while eukaryotic V-type ATPases function as ion pumps. To begin to rationalize the molecular basis for this functional differentiation, we solved the crystal structure of the Na+-driven membrane rotor of the Acetobacterium woodii ATP synthase, at 2.1 Å resolution. Unlike known structures, this rotor ring is a 9:1 heteromer of F- and V-type c-subunits, and therefore features a hybrid configuration of ion-binding sites along its circumference. Molecular and kinetic simulations are used to dissect the mechanisms of Na+ recognition and rotation of this c-ring, and to explain the functional implications of the V-type c-subunit. These structural and mechanistic insights indicate an evolutionary path between synthases and pumps involving adaptations in the rotor ring. PMID:25381992

A DNA methylation map of human cancer at single base-pair resolution.

PubMed

Vidal, E; Sayols, S; Moran, S; Guillaumet-Adkins, A; Schroeder, M P; Royo, R; Orozco, M; Gut, M; Gut, I; Lopez-Bigas, N; Heyn, H; Esteller, M

2017-10-05

Although single base-pair resolution DNA methylation landscapes for embryonic and different somatic cell types provided important insights into epigenetic dynamics and cell-type specificity, such comprehensive profiling is incomplete across human cancer types. This prompted us to perform genome-wide DNA methylation profiling of 22 samples derived from normal tissues and associated neoplasms, including primary tumors and cancer cell lines. Unlike their invariant normal counterparts, cancer samples exhibited highly variable CpG methylation levels in a large proportion of the genome, involving progressive changes during tumor evolution. The whole-genome sequencing results from selected samples were replicated in a large cohort of 1112 primary tumors of various cancer types using genome-scale DNA methylation analysis. Specifically, we determined DNA hypermethylation of promoters and enhancers regulating tumor-suppressor genes, with potential cancer-driving effects. DNA hypermethylation events showed evidence of positive selection, mutual exclusivity and tissue specificity, suggesting their active participation in neoplastic transformation. Our data highlight the extensive changes in DNA methylation that occur in cancer onset, progression and dissemination.

Differential Deposition Technique for Figure Corrections in Grazing Incidence X-ray Optics

NASA Technical Reports Server (NTRS)

Kilaru, Kiranmayee; Ramsey, Brian D.; Gubarev, Mikhail

2009-01-01

A differential deposition technique is being developed to correct the low- and mid-spatial-frequency deviations in the axial figure profile of Wolter type grazing incidence X-ray optics. These deviations arise due to various factors in the fabrication process and they degrade the performance of the optics by limiting the achievable angular resolution. In the differential deposition technique, material of varying thickness is selectively deposited along the length of the optic to minimize these deviations, thereby improving the overall figure. High resolution focusing optics being developed at MSFC for small animal radionuclide imaging are being coated to test the differential deposition technique. The required spatial resolution for these optics is 100 m. This base resolution is achievable with the regular electroform-nickel-replication fabrication technique used at MSFC. However, by improving the figure quality of the optics through differential deposition, we aim at significantly improving the resolution beyond this value.

Impact of tropical cyclones on modeled extreme wind-wave climate

DOE PAGES

Timmermans, Ben; Stone, Daithi; Wehner, Michael; ...

2017-02-16

Here, the effect of forcing wind resolution on the extremes of global wind-wave climate are investigated in numerical simulations. Forcing winds from the Community Atmosphere Model at horizontal resolutions of ~1.0° and ~0.25° are used to drive Wavewatch III. Differences in extreme wave height are found to manifest most strongly in tropical cyclone (TC) regions, emphasizing the need for high-resolution forcing in those areas. Comparison with observations typically show improvement in performance with increased forcing resolution, with a strong influence in the tail of the distribution, although simulated extremes can exceed observations. A simulation for the end of the 21stmore » century under a RCP 8.5 type emission scenario suggests further increases in extreme wave height in TC regions.« less

Impact of tropical cyclones on modeled extreme wind-wave climate

NASA Astrophysics Data System (ADS)

Timmermans, Ben; Stone, Dáithí; Wehner, Michael; Krishnan, Harinarayan

2017-02-01

The effect of forcing wind resolution on the extremes of global wind-wave climate are investigated in numerical simulations. Forcing winds from the Community Atmosphere Model at horizontal resolutions of ˜1.0° and ˜0.25° are used to drive Wavewatch III. Differences in extreme wave height are found to manifest most strongly in tropical cyclone (TC) regions, emphasizing the need for high-resolution forcing in those areas. Comparison with observations typically show improvement in performance with increased forcing resolution, with a strong influence in the tail of the distribution, although simulated extremes can exceed observations. A simulation for the end of the 21st century under a RCP 8.5 type emission scenario suggests further increases in extreme wave height in TC regions.

Low-cost, high-precision micro-lensed optical fiber providing deep-micrometer to deep-nanometer-level light focusing.

PubMed

Wen, Sy-Bor; Sundaram, Vijay M; McBride, Daniel; Yang, Yu

2016-04-15

A new type of micro-lensed optical fiber through stacking appropriate high-refractive microspheres at designed locations with respect to the cleaved end of an optical fiber is numerically and experimentally demonstrated. This new type of micro-lensed optical fiber can be precisely constructed with low cost and high speed. Deep micrometer-scale and submicrometer-scale far-field light spots can be achieved when the optical fibers are multimode and single mode, respectively. By placing an appropriate teardrop dielectric nanoscale scatterer at the far-field spot of this new type of micro-lensed optical fiber, a deep-nanometer near-field spot can also be generated with high intensity and minimum joule heating, which is valuable in high-speed, high-resolution, and high-power nanoscale detection compared with traditional near-field optical fibers containing a significant portion of metallic material.

Restoration of high-resolution AFM images captured with broken probes

NASA Astrophysics Data System (ADS)

Wang, Y. F.; Corrigan, D.; Forman, C.; Jarvis, S.; Kokaram, A.

2012-03-01

A type of artefact is induced by damage of the scanning probe when the Atomic Force Microscope (AFM) captures a material surface structure with nanoscale resolution. This artefact has a dramatic form of distortion rather than the traditional blurring artefacts. Practically, it is not easy to prevent the damage of the scanning probe. However, by using natural image deblurring techniques in image processing domain, a comparatively reliable estimation of the real sample surface structure can be generated. This paper introduces a novel Hough Transform technique as well as a Bayesian deblurring algorithm to remove this type of artefact. The deblurring result is successful at removing blur artefacts in the AFM artefact images. And the details of the fibril surface topography are well preserved.

High-Resolution Spectroscopy of some very Active Southern Stars

NASA Technical Reports Server (NTRS)

Soderblom, David R.; King, Jeremy R.; Henry, Todd J.

1998-01-01

We have obtained high-resolution echelle spectra of 18 solar-type stars that an earlier survey showed to have very high levels of Ca II H and K emission. Most of these stars belong to close binary systems, but five remain as probable single stars or well-separated binaries that are younger than the Pleiades on the basis of their lithium abundances and H.alpha emission. Three of these probable single stars also lie more than 1 mag above the main sequence in a color-magnitude diagram, and appear to have ages of 10 to 15 Myr. Two of them, HD 202917 and HD 222259, also appear to have a kinematic association with the pre-main-sequence multiple system HD 98800.

Etalon Array Reconstructive Spectrometry

NASA Astrophysics Data System (ADS)

Huang, Eric; Ma, Qian; Liu, Zhaowei

2017-01-01

Compact spectrometers are crucial in areas where size and weight may need to be minimized. These types of spectrometers often contain no moving parts, which makes for an instrument that can be highly durable. With the recent proliferation in low-cost and high-resolution cameras, camera-based spectrometry methods have the potential to make portable spectrometers small, ubiquitous, and cheap. Here, we demonstrate a novel method for compact spectrometry that uses an array of etalons to perform spectral encoding, and uses a reconstruction algorithm to recover the incident spectrum. This spectrometer has the unique capability for both high resolution and a large working bandwidth without sacrificing sensitivity, and we anticipate that its simplicity makes it an excellent candidate whenever a compact, robust, and flexible spectrometry solution is needed.

Rotary ATPases

PubMed Central

Stewart, Alastair G.; Sobti, Meghna; Harvey, Richard P.; Stock, Daniela

2013-01-01

Rotary ATPases are molecular rotary motors involved in biological energy conversion. They either synthesize or hydrolyze the universal biological energy carrier adenosine triphosphate. Recent work has elucidated the general architecture and subunit compositions of all three sub-types of rotary ATPases. Composite models of the intact F-, V- and A-type ATPases have been constructed by fitting high-resolution X-ray structures of individual subunits or sub-complexes into low-resolution electron densities of the intact enzymes derived from electron cryo-microscopy. Electron cryo-tomography has provided new insights into the supra-molecular arrangement of eukaryotic ATP synthases within mitochondria and mass-spectrometry has started to identify specifically bound lipids presumed to be essential for function. Taken together these molecular snapshots show that nano-scale rotary engines have much in common with basic design principles of man made machines from the function of individual “machine elements” to the requirement of the right “fuel” and “oil” for different types of motors. PMID:23369889

High-Resolution Mesoscale Simulations of the 6-7 May 2000 Missouri Flash Flood: Impact of Model Initialization and Land Surface Treatment

NASA Technical Reports Server (NTRS)

Baker, R. David; Wang, Yansen; Tao, Wei-Kuo; Wetzel, Peter; Belcher, Larry R.

2004-01-01

High-resolution mesoscale model simulations of the 6-7 May 2000 Missouri flash flood event were performed to test the impact of model initialization and land surface treatment on timing, intensity, and location of extreme precipitation. In this flash flood event, a mesoscale convective system (MCS) produced over 340 mm of rain in roughly 9 hours in some locations. Two different types of model initialization were employed: 1) NCEP global reanalysis with 2.5-degree grid spacing and 12-hour temporal resolution, and 2) Eta reanalysis with 40- km grid spacing and $hour temporal resolution. In addition, two different land surface treatments were considered. A simple land scheme. (SLAB) keeps soil moisture fixed at initial values throughout the simulation, while a more sophisticated land model (PLACE) allows for r interactive feedback. Simulations with high-resolution Eta model initialization show considerable improvement in the intensity of precipitation due to the presence in the initialization of a residual mesoscale convective vortex (hlCV) from a previous MCS. Simulations with the PLACE land model show improved location of heavy precipitation. Since soil moisture can vary over time in the PLACE model, surface energy fluxes exhibit strong spatial gradients. These surface energy flux gradients help produce a strong low-level jet (LLJ) in the correct location. The LLJ then interacts with the cold outflow boundary of the MCS to produce new convective cells. The simulation with both high-resolution model initialization and time-varying soil moisture test reproduces the intensity and location of observed rainfall.

High resolution remote sensing information identification for characterizing uranium mineralization setting in Namibia

NASA Astrophysics Data System (ADS)

Zhang, Jie-Lin; Wang, Jun-hu; Zhou, Mi; Huang, Yan-ju; Xuan, Yan-xiu; Wu, Ding

2011-11-01

The modern Earth Observation System (EOS) technology takes important role in the uranium geological exploration, and high resolution remote sensing as one of key parts of EOS is vital to characterize spectral and spatial information of uranium mineralization factors. Utilizing satellite high spatial resolution and hyperspectral remote sensing data (QuickBird, Radarsat2, ASTER), field spectral measurement (ASD data) and geological survey, this paper established the spectral identification characteristics of uranium mineralization factors including six different types of alaskite, lower and upper marble of Rössing formation, dolerite, alkali metasomatism, hematization and chloritization in the central zone of Damara Orogen, Namibia. Moreover, adopted the texture information identification technology, the geographical distribution zones of ore-controlling faults and boundaries between the different strata were delineated. Based on above approaches, the remote sensing geological anomaly information and image interpretation signs of uranium mineralization factors were extracted, the metallogenic conditions were evaluated, and the prospective areas have been predicted.

Direct observation of charged domain walls in hybrid improper ferroelectric (Ca,Sr)3Ti2O7

NASA Astrophysics Data System (ADS)

Kurushima, Kousuke; Yoshimoto, Wataru; Ishii, Yui; Cheong, Sang-Wook; Mori, Shigeo

2017-10-01

We investigated ferroelectric (FE) domain wall structures including “charged domain walls” of hybrid improper FE (Ca,Sr)3Ti2O7 at the subatomic resolution by dark-field transmission electron microscopy (TEM) and high-resolution state-of-the-art aberration-corrected high-angle annular-dark-field (HAADF) scanning transmission electron microscopy (STEM). Dark-field TEM and high-resolution HAADF-STEM images obtained in the FE phase of single crystals of Ca2.46Sr0.54Ti2O7 revealed the formation of abundant charged domain walls with the head-to-head and tail-to-tail configurations in the FE domain structure, in addition to the FE 180° domain structure. The charged domain walls with the head-to-head and tail-to-tail FE polarizations exist stably and can be characterized as the unique double arc-type displacement of Ca/Sr ions in a unit cell without charge accumulation.

What does C II lambda 2325 A emission tell us about chromospheres of red supergiants? - A critical test using Zeta Aurigae-type K supergiants

NASA Technical Reports Server (NTRS)

Schroeder, K.-P.; Reimers, D.; Carpenter, K. G.; Brown, A.

1988-01-01

The limitations of the Carpenter et al. (1985) C II intercombination multiplet method of determining the density and geometric extent of red giant chromospheres are presently tested through observation of the C II 2325 A emission of two K-type supergiants whose empirical model chromospheres have been derived by high-resolution IUE observations at eclipse phases. While the observed C II emission fluxes are well reproduced, much of this emission originates in the high-density lower chromosphere.

The System Design, Engineering Architecture, and Preliminary Results of a Lower-Cost High-Sensitivity High-Resolution Positron Emission Mammography Camera.

PubMed

Zhang, Yuxuan; Ramirez, Rocio A; Li, Hongdi; Liu, Shitao; An, Shaohui; Wang, Chao; Baghaei, Hossain; Wong, Wai-Hoi

2010-02-01

A lower-cost high-sensitivity high-resolution positron emission mammography (PEM) camera is developed. It consists of two detector modules with the planar detector bank of 20 × 12 cm(2). Each bank has 60 low-cost PMT-Quadrant-Sharing (PQS) LYSO blocks arranged in a 10 × 6 array with two types of geometries. One is the symmetric 19.36 × 19.36 mm(2) block made of 1.5 × 1.5 × 10 mm(3) crystals in a 12 × 12 array. The other is the 19.36 × 26.05 mm(2) asymmetric block made of 1.5 × 1.9 × 10 mm(3) crystals in 12 × 13 array. One row (10) of the elongated blocks are used along one side of the bank to reclaim the half empty PMT photocathode in the regular PQS design to reduce the dead area at the edge of the module. The bank has a high overall crystal packing fraction of 88%, which results in a very high sensitivity. Mechanical design and electronics have been developed for low-cost, compactness, and stability purposes. Each module has four Anger-HYPER decoding electronics that can handle a count-rate of 3 Mcps for single events. A simple two-module coincidence board with a hardware delay window for random coincidences has been developed with an adjustable window of 6 to 15 ns. Some of the performance parameters have been studied by preliminary tests and Monte Carlo simulations, including the crystal decoding map and the 17% energy resolution of the detectors, the point source sensitivity of 11.5% with 50 mm bank-to-bank distance, the 1.2 mm-spatial resolutions, 42 kcps peak Noise Equivalent Count Rate at 7.0-mCi total activity in human body, and the resolution phantom images. Those results show that the design goal of building a lower-cost, high-sensitivity, high-resolution PEM detector is achieved.

Resolution factors in edgeline holography.

PubMed

Trolinger, J D; Gee, T H

1971-06-01

When an in-line Fresnel hologram of an object such as a projectile in flight is made, the reconstruction comprises an image of the outside edge of the object superimposed upon a Fresnel diffraction pattern of the edge and an unmodulated portion of the reconstruction beam. When the reconstructed image is bandpass filtered, the only remaining significant contribution is that of a diffraction pattern which is symmetrical about an edgeline gaussian image of the object. The present paper discusses the application of this type of holography in accurately locating the edge of a large dynamic object, the position of which is not accurately known in any dimension. A theoretical and experimental analysis was performed to study the effects of motion, hologram size, film type, and practical limitations upon the attainable resolution in the reconstructed image. The bandlimiting effect of motion is used to relate the motion effected resolution limit of holography to that of photography. The study shows that an edgeline can be accurately located even at high velocity normal to the edge.

Comparison of GC stationary phases for the separation of fatty acid methyl esters in biodiesel fuels.

PubMed

Goding, Julian C; Ragon, Dorisanne Y; O'Connor, Jack B; Boehm, Sarah J; Hupp, Amber M

2013-07-01

The fatty acid methyl ester (FAME) content of biodiesel fuels has traditionally been determined using gas chromatography with a polar stationary phase. In this study, a direct comparison of the separation of FAMEs present in various biodiesel samples on three polar stationary phases and one moderately polar stationary phase (with comparable column dimensions) was performed. Retention on each column was based on solubility in and polarity of the phase. Quantitative metrics describing the resolution of important FAME pairs indicate high resolution on all polar columns, yet the best resolution, particularly of geometric isomers, is achieved on the cyanopropyl column. In addition, the separation of four C18 monounsaturated isomers was optimized and the elution order determined on each column. FAME composition of various biodiesel fuel types was determined on each column to illustrate (1) chemical differences in biodiesels produced from different feedstocks and (2) chemical similarities in biodiesels of the same feedstock type produced in different locations and harvest seasons.

FlyMAD: rapid thermogenetic control of neuronal activity in freely walking Drosophila.

PubMed

Bath, Daniel E; Stowers, John R; Hörmann, Dorothea; Poehlmann, Andreas; Dickson, Barry J; Straw, Andrew D

2014-07-01

Rapidly and selectively modulating the activity of defined neurons in unrestrained animals is a powerful approach in investigating the circuit mechanisms that shape behavior. In Drosophila melanogaster, temperature-sensitive silencers and activators are widely used to control the activities of genetically defined neuronal cell types. A limitation of these thermogenetic approaches, however, has been their poor temporal resolution. Here we introduce FlyMAD (the fly mind-altering device), which allows thermogenetic silencing or activation within seconds or even fractions of a second. Using computer vision, FlyMAD targets an infrared laser to freely walking flies. As a proof of principle, we demonstrated the rapid silencing and activation of neurons involved in locomotion, vision and courtship. The spatial resolution of the focused beam enabled preferential targeting of neurons in the brain or ventral nerve cord. Moreover, the high temporal resolution of FlyMAD allowed us to discover distinct timing relationships for two neuronal cell types previously linked to courtship song.

Experimental assessment and analysis of super-resolution in fluorescence microscopy based on multiple-point spread function fitting of spectrally demultiplexed images

NASA Astrophysics Data System (ADS)

Nishimura, Takahiro; Kimura, Hitoshi; Ogura, Yusuke; Tanida, Jun

2018-06-01

This paper presents an experimental assessment and analysis of super-resolution microscopy based on multiple-point spread function fitting of spectrally demultiplexed images using a designed DNA structure as a test target. For the purpose, a DNA structure was designed to have binding sites at a certain interval that is smaller than the diffraction limit. The structure was labeled with several types of quantum dots (QDs) to acquire their spatial information as spectrally encoded images. The obtained images are analyzed with a point spread function multifitting algorithm to determine the QD locations that indicate the binding site positions. The experimental results show that the labeled locations can be observed beyond the diffraction-limited resolution using three-colored fluorescence images that were obtained with a confocal fluorescence microscope. Numerical simulations show that labeling with eight types of QDs enables the positions aligned at 27.2-nm pitches on the DNA structure to be resolved with high accuracy.

Coastal habitat mapping in the Aegean Sea using high resolution orthophoto maps

NASA Astrophysics Data System (ADS)

Topouzelis, Konstantinos; Papakonstantinou, Apostolos; Doukari, Michaela; Stamatis, Panagiotis; Makri, Despina; Katsanevakis, Stelios

2017-09-01

The significance of coastal habitat mapping lies in the need to prevent from anthropogenic interventions and other factors. Until 2015, Landsat-8 (30m) imagery were used as medium spatial resolution satellite imagery. So far, Sentinel-2 satellite imagery is very useful for more detailed regional scale mapping. However, the use of high resolution orthophoto maps, which are determined from UAV data, is expected to improve the mapping accuracy. This is due to small spatial resolution of the orthophoto maps (30 cm). This paper outlines the integration of UAS for data acquisition and Structure from Motion (SfM) pipeline for the visualization of selected coastal areas in the Aegean Sea. Additionally, the produced orthophoto maps analyzed through an object-based image analysis (OBIA) and nearest-neighbor classification for mapping the coastal habitats. Classification classes included the main general habitat types, i.e. seagrass, soft bottom, and hard bottom The developed methodology applied at the Koumbara beach (Ios Island - Greece). Results showed that UAS's data revealed the sub-bottom complexity in large shallow areas since they provide such information in the spatial resolution that permits the mapping of seagrass meadows with extreme detail. The produced habitat vectors are ideal as reference data for studies with satellite data of lower spatial resolution.

Comparison of alternative spatial resolutions in the application of a spatially distributed biogeochemical model over complex terrain

USGS Publications Warehouse

Turner, D.P.; Dodson, R.; Marks, D.

1996-01-01

Spatially distributed biogeochemical models may be applied over grids at a range of spatial resolutions, however, evaluation of potential errors and loss of information at relatively coarse resolutions is rare. In this study, a georeferenced database at the 1-km spatial resolution was developed to initialize and drive a process-based model (Forest-BGC) of water and carbon balance over a gridded 54976 km2 area covering two river basins in mountainous western Oregon. Corresponding data sets were also prepared at 10-km and 50-km spatial resolutions using commonly employed aggregation schemes. Estimates were made at each grid cell for climate variables including daily solar radiation, air temperature, humidity, and precipitation. The topographic structure, water holding capacity, vegetation type and leaf area index were likewise estimated for initial conditions. The daily time series for the climatic drivers was developed from interpolations of meteorological station data for the water year 1990 (1 October 1989-30 September 1990). Model outputs at the 1-km resolution showed good agreement with observed patterns in runoff and productivity. The ranges for model inputs at the 10-km and 50-km resolutions tended to contract because of the smoothed topography. Estimates for mean evapotranspiration and runoff were relatively insensitive to changing the spatial resolution of the grid whereas estimates of mean annual net primary production varied by 11%. The designation of a vegetation type and leaf area at the 50-km resolution often subsumed significant heterogeneity in vegetation, and this factor accounted for much of the difference in the mean values for the carbon flux variables. Although area wide means for model outputs were generally similar across resolutions, difference maps often revealed large areas of disagreement. Relatively high spatial resolution analyses of biogeochemical cycling are desirable from several perspectives and may be particularly important in the study of the potential impacts of climate change.

High-resolution HLA haplotype frequencies of stem cell donors in Germany with foreign parentage: how can they be used to improve unrelated donor searches?

PubMed

Pingel, Julia; Solloch, Ute V; Hofmann, Jan A; Lange, Vinzenz; Ehninger, Gerhard; Schmidt, Alexander H

2013-03-01

In hematopoietic stem cell transplantation, human leukocyte antigens (HLA), usually HLA loci A, B, C, DRB1 and DQB1, are required to check histocompatibility between a potential donor and the recipient suffering from a malignant or non-malignant blood disease. As databases of potential unrelated donors are very heterogeneous with respect to typing resolution and number of typed loci, donor registries make use of haplotype frequency-based algorithms to provide matching probabilities for each potentially matching recipient/donor pair. However, it is well known that HLA allele and haplotype frequencies differ significantly between populations. We estimated high-resolution HLA-A, -B, -C, -DRB1 haplotype and allele frequencies of donors within DKMS German Bone Marrow Donor Center with parentage from 17 different countries: Turkey, Poland, Italy, Russian Federation, Croatia, Greece, Austria, Kazakhstan, France, The Netherlands, Republic of China, Romania, Portugal, USA, Spain, United Kingdom and Bosnia and Herzegovina. 5-locus haplotypes including HLA-DQB1 are presented for Turkey, Poland, Italy and Russian Federation. We calculated linkage disequilibria for each sample. Genetic distances between included countries could be shown to reflect geography. We further demonstrate how genetic differences between populations are reflected in matching probabilities of recipient/donor pairs and how they influence the search for unrelated donors as well as strategic donor center typings. Copyright © 2012 American Society for Histocompatibility and Immunogenetics. Published by Elsevier Inc. All rights reserved.

Analysis and classification of commercial ham slice images using directional fractal dimension features.

PubMed

Mendoza, Fernando; Valous, Nektarios A; Allen, Paul; Kenny, Tony A; Ward, Paddy; Sun, Da-Wen

2009-02-01

This paper presents a novel and non-destructive approach to the appearance characterization and classification of commercial pork, turkey and chicken ham slices. Ham slice images were modelled using directional fractal (DF(0°;45°;90°;135°)) dimensions and a minimum distance classifier was adopted to perform the classification task. Also, the role of different colour spaces and the resolution level of the images on DF analysis were investigated. This approach was applied to 480 wafer thin ham slices from four types of hams (120 slices per type): i.e., pork (cooked and smoked), turkey (smoked) and chicken (roasted). DF features were extracted from digitalized intensity images in greyscale, and R, G, B, L(∗), a(∗), b(∗), H, S, and V colour components for three image resolution levels (100%, 50%, and 25%). Simulation results show that in spite of the complexity and high variability in colour and texture appearance, the modelling of ham slice images with DF dimensions allows the capture of differentiating textural features between the four commercial ham types. Independent DF features entail better discrimination than that using the average of four directions. However, DF dimensions reveal a high sensitivity to colour channel, orientation and image resolution for the fractal analysis. The classification accuracy using six DF dimension features (a(90°)(∗),a(135°)(∗),H(0°),H(45°),S(0°),H(90°)) was 93.9% for training data and 82.2% for testing data.

High-resolution synchrotron X-ray analysis of bioglass-enriched hydrogels.

PubMed

Gorodzha, Svetlana; Douglas, Timothy E L; Samal, Sangram K; Detsch, Rainer; Cholewa-Kowalska, Katarzyna; Braeckmans, Kevin; Boccaccini, Aldo R; Skirtach, Andre G; Weinhardt, Venera; Baumbach, Tilo; Surmeneva, Maria A; Surmenev, Roman A

2016-05-01

Enrichment of hydrogels with inorganic particles improves their suitability for bone regeneration by enhancing their mechanical properties, mineralizability, and bioactivity as well as adhesion, proliferation, and differentiation of bone-forming cells, while maintaining injectability. Low aggregation and homogeneous distribution maximize particle surface area, promoting mineralization, cell-particle interactions, and homogenous tissue regeneration. Hence, determination of the size and distribution of particles/particle agglomerates in the hydrogel is desirable. Commonly used techniques have drawbacks. High-resolution techniques (e.g., SEM) require drying. Distribution in the dry state is not representative of the wet state. Techniques in the wet state (histology, µCT) are of lower resolution. Here, self-gelling, injectable composites of Gellan Gum (GG) hydrogel and two different types of sol-gel-derived bioactive glass (bioglass) particles were analyzed in the wet state using Synchrotron X-ray radiation, enabling high-resolution determination of particle size and spatial distribution. The lower detection limit volume was 9 × 10(-5) mm(3) . Bioglass particle suspensions were also studied using zeta potential measurements and Coulter analysis. Aggregation of bioglass particles in the GG hydrogels occurred and aggregate distribution was inhomogeneous. Bioglass promoted attachment of rat mesenchymal stem cells (rMSC) and mineralization. © 2016 Wiley Periodicals, Inc.

High temporal resolution of extreme rainfall rate variability and the acoustic classification of rainfall

NASA Astrophysics Data System (ADS)

Nystuen, Jeffrey A.; Amitai, Eyal

2003-04-01

The underwater sound generated by raindrop splashes on a water surface is loud and unique allowing detection, classification and quantification of rainfall. One of the advantages of the acoustic measurement is that the listening area, an effective catchment area, is proportional to the depth of the hydrophone and can be orders of magnitude greater than other in situ rain gauges. This feature allows high temporal resolution of the rainfall measurement. A series of rain events with extremely high rainfall rates, over 100 mm/hr, is examined acoustically. Rapid onset and cessation of rainfall intensity are detected within the convective cells of these storms with maximum 5-s resolution values exceeding 1000 mm/hr. The probability distribution functions (pdf) for rainfall rate occurrence and water volume using the longer temporal resolutions typical of other instruments do not include these extreme values. The variance of sound intensity within different acoustic frequency bands can be used as an aid to classify rainfall type. Objective acoustic classification algorithms are proposed. Within each rainfall classification the relationship between sound intensity and rainfall rate is nearly linear. The reflectivity factor, Z, also has a linear relationship with rainfall rate, R, for each rainfall classification.

Defining habitat covariates in camera-trap based occupancy studies

PubMed Central

Niedballa, Jürgen; Sollmann, Rahel; Mohamed, Azlan bin; Bender, Johannes; Wilting, Andreas

2015-01-01

In species-habitat association studies, both the type and spatial scale of habitat covariates need to match the ecology of the focal species. We assessed the potential of high-resolution satellite imagery for generating habitat covariates using camera-trapping data from Sabah, Malaysian Borneo, within an occupancy framework. We tested the predictive power of covariates generated from satellite imagery at different resolutions and extents (focal patch sizes, 10–500 m around sample points) on estimates of occupancy patterns of six small to medium sized mammal species/species groups. High-resolution land cover information had considerably more model support for small, patchily distributed habitat features, whereas it had no advantage for large, homogeneous habitat features. A comparison of different focal patch sizes including remote sensing data and an in-situ measure showed that patches with a 50-m radius had most support for the target species. Thus, high-resolution satellite imagery proved to be particularly useful in heterogeneous landscapes, and can be used as a surrogate for certain in-situ measures, reducing field effort in logistically challenging environments. Additionally, remote sensed data provide more flexibility in defining appropriate spatial scales, which we show to impact estimates of wildlife-habitat associations. PMID:26596779

North Atlantic Tropical Cyclones: historical simulations and future changes with the new high-resolution Arpege AGCM.

NASA Astrophysics Data System (ADS)

Pilon, R.; Chauvin, F.; Palany, P.; Belmadani, A.

2017-12-01

A new version of the variable high-resolution Meteo-France Arpege atmospheric general circulation model (AGCM) has been developed for tropical cyclones (TC) studies, with a focus on the North Atlantic basin, where the model horizontal resolution is 15 km. Ensemble historical AMIP (Atmospheric Model Intercomparison Project)-type simulations (1965-2014) and future projections (2020-2080) under the IPCC (Intergovernmental Panel on Climate Change) representative concentration pathway (RCP) 8.5 scenario have been produced. TC-like vortices tracking algorithm is used to investigate TC activity and variability. TC frequency, genesis, geographical distribution and intensity are examined. Historical simulations are compared to best-track and reanalysis datasets. Model TC frequency is generally realistic but tends to be too high during the rst decade of the historical simulations. Biases appear to originate from both the tracking algorithm and model climatology. Nevertheless, the model is able to simulate extremely well intense TCs corresponding to category 5 hurricanes in the North Atlantic, where grid resolution is highest. Interaction between developing TCs and vertical wind shear is shown to be contributing factor for TC variability. Future changes in TC activity and properties are also discussed.

Results from a new die-to-database reticle inspection platform

NASA Astrophysics Data System (ADS)

Broadbent, William; Xiong, Yalin; Giusti, Michael; Walsh, Robert; Dayal, Aditya

2007-03-01

A new die-to-database high-resolution reticle defect inspection system has been developed for the 45nm logic node and extendable to the 32nm node (also the comparable memory nodes). These nodes will use predominantly 193nm immersion lithography although EUV may also be used. According to recent surveys, the predominant reticle types for the 45nm node are 6% simple tri-tone and COG. Other advanced reticle types may also be used for these nodes including: dark field alternating, Mask Enhancer, complex tri-tone, high transmission, CPL, EUV, etc. Finally, aggressive model based OPC will typically be used which will include many small structures such as jogs, serifs, and SRAF (sub-resolution assist features) with accompanying very small gaps between adjacent structures. The current generation of inspection systems is inadequate to meet these requirements. The architecture and performance of a new die-to-database inspection system is described. This new system is designed to inspect the aforementioned reticle types in die-to-database and die-to-die modes. Recent results from internal testing of the prototype systems are shown. The results include standard programmed defect test reticles and advanced 45nm and 32nm node reticles from industry sources. The results show high sensitivity and low false detections being achieved.

Classification of sea ice types with single-band (33.6 GHz) airborne passive microwave imagery

NASA Astrophysics Data System (ADS)

Eppler, Duane T.; Farmer, L. Dennis; Lohanick, Alan W.; Hoover, Mervyn

1986-09-01

During March 1983 extensive high-quality airborne passive Ka band (33.6 GHz) microwave imagery and coincident high-resolution aerial photography were obtained of ice along a 378-km flight line in the Beaufort Sea. Analysis of these data suggests that four classes of winter surfaces can be distinguished solely on the basis of 33.6-GHz brightness temperature: open water, frazil, old ice, and young/first-year ice. New ice (excluding frazil) and nilas display brightness temperatures that overlap the range of temperatures characteristic of old ice and, to a lesser extent, young/first-year ice. Scenes in which a new ice or nilas are present in appreciable amounts are subject to substantial errors in classification if static measures of Ka band radiometric brightness temperature alone are considered. Textural characteristics of nilas and new ice, however, differ significantly from textural features characteristic of other ice types and probably can be used with brightness temperature data to classify ice type in high-resolution single-band microwave images. In any case, open water is radiometrically the coldest surface observed in any scene. Lack of overlap between brightness temperatures characteristic of other surfaces indicates that estimates of the areal extent of open water based on only 33.6-GHz brightness temperatures are accurate.

High-speed high-resolution epifluorescence imaging system using CCD sensor and digital storage for neurobiological research

NASA Astrophysics Data System (ADS)

Takashima, Ichiro; Kajiwara, Riichi; Murano, Kiyo; Iijima, Toshio; Morinaka, Yasuhiro; Komobuchi, Hiroyoshi

2001-04-01

We have designed and built a high-speed CCD imaging system for monitoring neural activity in an exposed animal cortex stained with a voltage-sensitive dye. Two types of custom-made CCD sensors were developed for this system. The type I chip has a resolution of 2664 (H) X 1200 (V) pixels and a wide imaging area of 28.1 X 13.8 mm, while the type II chip has 1776 X 1626 pixels and an active imaging area of 20.4 X 18.7 mm. The CCD arrays were constructed with multiple output amplifiers in order to accelerate the readout rate. The two chips were divided into either 24 (I) or 16 (II) distinct areas that were driven in parallel. The parallel CCD outputs were digitized by 12-bit A/D converters and then stored in the frame memory. The frame memory was constructed with synchronous DRAM modules, which provided a capacity of 128 MB per channel. On-chip and on-memory binning methods were incorporated into the system, e.g., this enabled us to capture 444 X 200 pixel-images for periods of 36 seconds at a rate of 500 frames/second. This system was successfully used to visualize neural activity in the cortices of rats, guinea pigs, and monkeys.

Validation of Atmospheric InfraRed Sounder (AIRS) spectral radiances with the Scanning High-resolution Interferometer Sounder (S-HIS) aircraft instrument

NASA Astrophysics Data System (ADS)

Tobin, David C.; Revercomb, Henry E.; Moeller, Chris C.; Knuteson, Robert O.; Best, Fred A.; Smith, William L.; van Delst, Paul; LaPorte, Daniel D.; Ellington, Scott D.; Werner, Mark D.; Dedecker, Ralph G.; Garcia, Raymond K.; Ciganovich, Nick N.; Howell, Hugh B.; Dutcher, Steven B.; Taylor, Joe K.

2004-11-01

The ability to accurately validate high spectral resolution infrared radiance measurements from space using comparisons with aircraft spectrometer observations has been successfully demonstrated. The demonstration is based on an under-flight of the Atmospheric Infrared Sounder (AIRS) on the NASA Aqua spacecraft by the Scanning High resolution Interferometer Sounder (S-HIS) on the NASA ER-2 high altitude aircraft on 21 November 2002 and resulted in brightness temperature differences approaching 0.1K for most of the spectrum. This paper presents the details of this AIRS/S-HIS validation case and also presents comparisons of Aqua AIRS and Moderate Resolution Imaging Spectroradiometer (MODIS) radiance observations. Aircraft comparisons of this type provide a mechanism for periodically testing the absolute calibration of spacecraft instruments with instrumentation for which the calibration can be carefully maintained on the ground. This capability is especially valuable for assuring the long-term consistency and accuracy of climate observations. It is expected that aircraft flights of the S-HIS and its close cousin the National Polar Orbiting Environmental Satellite System (NPOESS) Atmospheric Sounder Testbed (NAST) will be used to check the long-term stability of the NASA EOS spacecrafts (Terra, Aqua and Aura) and the follow-on complement of operational instruments, including the Cross-track Infrared Sounder (CrIS).

High Resolution Crystal Structure of Human β-Glucuronidase Reveals Structural Basis of Lysosome Targeting

PubMed Central

Hassan, Md. Imtaiyaz; Waheed, Abdul; Grubb, Jeffery H.; Klei, Herbert E.; Korolev, Sergey; Sly, William S.

2013-01-01

Human β-glucuronidase (GUS) cleaves β-D-glucuronic acid residues from the non-reducing termini of glycosaminoglycan and its deficiency leads to mucopolysaccharidosis type VII (MPSVII). Here we report a high resolution crystal structure of human GUS at 1.7 Å resolution and present an extensive analysis of the structural features, unifying recent findings in the field of lysosome targeting and glycosyl hydrolases. The structure revealed several new details including a new glycan chain at Asn272, in addition to that previously observed at Asn173, and coordination of the glycan chain at Asn173 with Lys197 of the lysosomal targeting motif which is essential for phosphotransferase recognition. Analysis of the high resolution structure not only provided new insights into the structural basis for lysosomal targeting but showed significant differences between human GUS, which is medically important in its own right, and E. coli GUS, which can be selectively inhibited in the human gut to prevent prodrug activation and is also widely used as a reporter gene by plant biologists. Despite these differences, both human and E. coli GUS share a high structure homology in all three domains with most of the glycosyl hydrolases, suggesting that they all evolved from a common ancestral gene. PMID:24260279

Association of High-Resolution Manometry Metrics with the Symptoms of Achalasia and the Symptomatic Outcomes of Peroral Esophageal Myotomy

PubMed Central

Tang, Yurong; Xie, Chen; Wang, Meifeng; Jiang, Liuqin; Shi, Ruihua; Lin, Lin

2015-01-01

Background High-resolution manometry (HRM) has improved the accuracy of manometry in detecting achalasia and has helped distinguish between clinically relevant subtypes. This study investigated whether HRM metrics correlate with the achalasia symptoms and symptomatic outcomes of peroral esophageal myotomy (POEM). Methods Of the 30 patients who were enrolled, 25 were treated with POEM, 12 of who underwent HRM after 3 months. All the patients completed the Eckardt score questionnaires, and those who underwent POEM were followed up for about 6 months. Pearson correlation was used to assess the relationship between the HRM metrics and symptoms and outcomes. Key results The integrated relaxation pressure (IRP) score positively correlated with the total Eckardt score, regurgitation score and weight loss score in all the patients, and with the weight loss score in type I achalasia. In 25 patients (10 patients, type I; 15 patients, type II) who underwent POEM, the total Eckardt scores and individual symptom scores significantly decreased after surgery. Changes in the Eckardt scores were similar between type I and type II. Further, the Eckardt scores and weight loss score changes were positively correlated with baseline IRP. Twelve patients (4 patients, type I; 8 patients, type II) underwent HRM again after POEM. IRP changed significantly after POEM, as did the DEP in type II. The IRP changes after POEM were positively correlated with the Eckardt score changes. Conclusions & Inferences IRP is correlated with the symptoms and outcomes of achalasia patients. Thus, HRM is effective for assessing the severity of achalasia and can predict the efficacy of POEM. PMID:26421919

Association of High-Resolution Manometry Metrics with the Symptoms of Achalasia and the Symptomatic Outcomes of Peroral Esophageal Myotomy.

PubMed

Tang, Yurong; Xie, Chen; Wang, Meifeng; Jiang, Liuqin; Shi, Ruihua; Lin, Lin

2015-01-01

High-resolution manometry (HRM) has improved the accuracy of manometry in detecting achalasia and has helped distinguish between clinically relevant subtypes. This study investigated whether HRM metrics correlate with the achalasia symptoms and symptomatic outcomes of peroral esophageal myotomy (POEM). Of the 30 patients who were enrolled, 25 were treated with POEM, 12 of who underwent HRM after 3 months. All the patients completed the Eckardt score questionnaires, and those who underwent POEM were followed up for about 6 months. Pearson correlation was used to assess the relationship between the HRM metrics and symptoms and outcomes. The integrated relaxation pressure (IRP) score positively correlated with the total Eckardt score, regurgitation score and weight loss score in all the patients, and with the weight loss score in type I achalasia. In 25 patients (10 patients, type I; 15 patients, type II) who underwent POEM, the total Eckardt scores and individual symptom scores significantly decreased after surgery. Changes in the Eckardt scores were similar between type I and type II. Further, the Eckardt scores and weight loss score changes were positively correlated with baseline IRP. Twelve patients (4 patients, type I; 8 patients, type II) underwent HRM again after POEM. IRP changed significantly after POEM, as did the DEP in type II. The IRP changes after POEM were positively correlated with the Eckardt score changes. IRP is correlated with the symptoms and outcomes of achalasia patients. Thus, HRM is effective for assessing the severity of achalasia and can predict the efficacy of POEM.

Clinical, Endoscopic, and Radiologic Features of Three Subtypes of Achalasia, Classified Using High-Resolution Manometry

PubMed Central

Khan, Mohammed Q.; AlQaraawi, Abdullah; Al-Sohaibani, Fahad; Al-Kahtani, Khalid; Al-Ashgar, Hamad I.

2015-01-01

Background/Aims: High-resolution manometry (HRM) has improved the accuracy of manometry in detecting achalasia and determining its subtypes. However, the correlation of achalasia subtypes with clinical, endoscopic, and radiologic findings has not been assessed. We aimed to evaluate and compare the clinical, endoscopic, and fluoroscopy findings associated with three subtypes of achalasia using HRM. Patients and Methods: The retrospective clinical data, HRM, endoscopy, and radiologic findings were obtained from the medical records of untreated achalasia patients. Results: From 2011 to 2013, 374 patients underwent HRM. Fifty-two patients (14%) were diagnosed with achalasia, but only 32 (8.5%) of these patients had not received treatment and were therefore included in this study. The endoscopy results were normal in 28% of the patients, and a barium swallow was inconclusive in 31% of the achalasia patients. Ten patients (31%) were classified as having type I achalasia, 17 (53%) were classified as type II, and 5 (16%) were classified as type III. Among the three subtypes, type I patients were on average the youngest and had the longest history of dysphagia, mildest chest pain, most significant weight loss, and most dilated esophagus with residual food. Chest pain was most common in type III patients, and frequently had normal fluoroscopic and endoscopic results. Conclusion: The clinical, radiologic, and endoscopic findings were not significantly different between patients with type I and type II untreated achalasia. Type III patients had the most severe symptoms and were the most difficult to diagnose based on varied clinical, radiologic, and endoscopic findings. PMID:26021774

SIL-STED microscopy technique enhancing super-resolution of fluorescence microscopy

NASA Astrophysics Data System (ADS)

Park, No-Cheol; Lim, Geon; Lee, Won-sup; Moon, Hyungbae; Choi, Guk-Jong; Park, Young-Pil

2017-08-01

We have characterized a new type STED microscope which combines a high numerical aperture (NA) optical head with a solid immersion lens (SIL), and we call it as SIL-STED microscope. The advantage of a SIL-STED microscope is that its high NA of the SIL makes it superior to a general STED microscope in lateral resolution, thus overcoming the optical diffraction limit at the macromolecular level and enabling advanced super-resolution imaging of cell surface or cell membrane structure and function Do. This study presents the first implementation of higher NA illumination in a STED microscope limiting the fluorescence lateral resolution to about 40 nm. The refractive index of the SIL which is made of material KTaO3 is about 2.23 and 2.20 at a wavelength of 633 nm and 780 nm which are used for excitation and depletion in STED imaging, respectively. Based on the vector diffraction theory, the electric field focused by the SILSTED microscope is numerically calculated so that the numerical results of the point dispersion function of the microscope and the expected resolution could be analyzed. For further investigation, fluorescence imaging of nano size fluorescent beads is fulfilled to show improved performance of the technique.

Further developments of series-connected superconducting tunnel junction to radiation detection

NASA Astrophysics Data System (ADS)

Kurakado, Masahiko; Ohsawa, Daisuke; Katano, Rintaro; Ito, Shin; Isozumi, Yasuhito

1997-10-01

One of the promising radiation detection devices for various practical applications is the series-connected superconducting tunnel junction (STJ) detector. In this article, interesting topics of the detectors are described since our previous work: e.g., more than two order higher detection efficiency compared with single STJ detectors, high count rate detection, and position resolution. Detectors were cooled to 0.35-0.4 K by means of a convenient 3He cryostat. The 5.9 and 6.5 keV x rays from 55Fe are separated by a detector specially designed for x-ray detection. The possible count rate of the series-junction detector estimated from the shaping-time constant applied in the measurements is high, e.g., over 104 counts per second. A series-junction detector equipped with a position sensing mechanism has shown a position resolution of about 35 μm in a sensing area with a radius of 1.1 mm. The position resolution of series junctions improves the energy resolution. A new type series-connected STJ detector is also proposed, i.e., the dispersed multitrap series-junction detector, for further improvement of detection efficiency and energy resolution.

Digital data from the Great Sand Dunes airborne gravity gradient survey, south-central Colorado

USGS Publications Warehouse

Drenth, B.J.; Abraham, J.D.; Grauch, V.J.S.; Labson, V.F.; Hodges, G.

2013-01-01

This report contains digital data and supporting explanatory files describing data types, data formats, and survey procedures for a high-resolution airborne gravity gradient (AGG) survey at Great Sand Dunes National Park, Alamosa and Saguache Counties, south-central Colorado. In the San Luis Valley, the Great Sand Dunes survey covers a large part of Great Sand Dunes National Park and Preserve. The data described were collected from a high-resolution AGG survey flown in February 2012, by Fugro Airborne Surveys Corp., on contract to the U.S. Geological Survey. Scientific objectives of the AGG survey are to investigate the subsurface structural framework that may influence groundwater hydrology and seismic hazards, and to investigate AGG methods and resolution using different flight specifications. Funding was provided by an airborne geophysics training program of the U.S. Department of Defense's Task Force for Business & Stability Operations.

Electric field imaging of single atoms

PubMed Central

Shibata, Naoya; Seki, Takehito; Sánchez-Santolino, Gabriel; Findlay, Scott D.; Kohno, Yuji; Matsumoto, Takao; Ishikawa, Ryo; Ikuhara, Yuichi

2017-01-01

In scanning transmission electron microscopy (STEM), single atoms can be imaged by detecting electrons scattered through high angles using post-specimen, annular-type detectors. Recently, it has been shown that the atomic-scale electric field of both the positive atomic nuclei and the surrounding negative electrons within crystalline materials can be probed by atomic-resolution differential phase contrast STEM. Here we demonstrate the real-space imaging of the (projected) atomic electric field distribution inside single Au atoms, using sub-Å spatial resolution STEM combined with a high-speed segmented detector. We directly visualize that the electric field distribution (blurred by the sub-Å size electron probe) drastically changes within the single Au atom in a shape that relates to the spatial variation of total charge density within the atom. Atomic-resolution electric field mapping with single-atom sensitivity enables us to examine their detailed internal and boundary structures. PMID:28555629

Deconvolving the Nucleus of Centaurus A Using Chandra PSF Library

NASA Technical Reports Server (NTRS)

Karovska, Margarita

2000-01-01

Centaurus A (NGC 5128) is a giant early-type galaxy containing the nearest (at 3.5 Mpc) radio-bright Active Galactic Nucleus (AGN). Cen A was observed with the High Resolution Camera (HRC) on the Chandra X-ray Observatory on several occasions since the launch in July 1999. The high-angular resolution (less than 0.5 arcsecond) Chandra/HRC images reveal X ray multi-scale structures in this object with unprecedented detail and clarity, including the bright nucleus believed to be associated with a supermassive black hole. We explored the spatial extent of the Cen A nucleus using deconvolution techniques on the full resolution Chandra images. Model point spread functions (PSFs) were derived from the standard Chandra raytrace PSF library as well as unresolved point sources observed with Chandra. The deconvolved images show that the Cen A nucleus is resolved and asymmetric. We discuss several possible causes of this extended emission and of the asymmetries.

High resolution FTIR spectrum of the ν 6 band of deuterated formic acid (DCOOH)

NASA Astrophysics Data System (ADS)

Goh, K. L.; Ong, P. P.; Tan, T. L.; Teo, H. H.

1999-07-01

The high resolution FTIR spectrum of the ν 6 band of DCOOH has been measured with a resolution of 0.004 cm -1 in the spectral range of 920-1020 cm -1. The ν 6 band was found to be perturbed by the neighbouring ν 8 band situated at about 100 cm -1 lower. Using a Watson's A-reduced Hamiltonian in the Ir representation, and with the inclusion of a-, and b-Coriolis coupling constants, a simultaneous fit of ν 6 and ν 8 was performed, fitting a total of 1656 infrared transitions of ν 6 with an rms uncertainty of 0.00038 cm -1. A set of accurate rovibrational constants for ν 6 were obtained. The ν 6 band was analysed to be primarily A-typed with a band centre at 970.88931±0.00003 cm -1.

Photoacoustic imaging of single circulating melanoma cells in vivo

NASA Astrophysics Data System (ADS)

Wang, Lidai; Yao, Junjie; Zhang, Ruiying; Xu, Song; Li, Guo; Zou, Jun; Wang, Lihong V.

2015-03-01

Melanoma, one of the most common types of skin cancer, has a high mortality rate, mainly due to a high propensity for tumor metastasis. The presence of circulating tumor cells (CTCs) is a potential predictor for metastasis. Label-free imaging of single circulating melanoma cells in vivo provides rich information on tumor progress. Here we present photoacoustic microscopy of single melanoma cells in living animals. We used a fast-scanning optical-resolution photoacoustic microscope to image the microvasculature in mouse ears. The imaging system has sub-cellular spatial resolution and works in reflection mode. A fast-scanning mirror allows the system to acquire fast volumetric images over a large field of view. A 500-kHz pulsed laser was used to image blood and CTCs. Single circulating melanoma cells were imaged in both capillaries and trunk vessels in living animals. These high-resolution images may be used in early detection of CTCs with potentially high sensitivity. In addition, this technique enables in vivo study of tumor cell extravasation from a primary tumor, which addresses an urgent pre-clinical need.

Development of Forest Drought Index and Forest Water Use Prediction in Gyeonggi Province, Korea Using High-Resolution Weather Research and Forecast Data and Localized JULES Land Surface Model

NASA Astrophysics Data System (ADS)

Lee, H.; Park, J.; Cho, S.; Lee, S. J.; Kim, H. S.

2017-12-01

Forest determines the amount of water available to low land ecosystems, which use the rest of water after evapotranspiration by forests. Substantial increase of drought, especially for seasonal drought, has occurred in Korea due to climate change, recently. To cope with this increasing crisis, it is necessary to predict the water use of forest. In our study, forest water use in the Gyeonggi Province in Korea was estimated using high-resolution (spatial and temporal) meteorological forecast data and localized Joint UK Land Environment Simulator (JULES) which is one of the widely used land surface models. The modeled estimation was used for developing forest drought index. The localization of the model was conducted by 1) refining the existing two tree plant functional types (coniferous and deciduous trees) into five (Quercus spp., other deciduous tree spp., Pinus spp., Larix spp., and other coniferous spp.), 2) correcting moderate resolution imaging spectroradiometer (MODIS) leaf area index (LAI) through data assimilation with in situ measured LAI, and 3) optimizing the unmeasured plant physiological parameters (e.g. leaf nitrogen contents, nitrogen distribution within canopy, light use efficiency) based on sensitivity analysis of model output values. The high-resolution (hourly and 810 × 810 m) National Center for AgroMeteorology-Land-Atmosphere Modeling Package (NCAM-LAMP) data were employed as meteorological input data in JULES. The plant functional types and soil texture of each grid cell in the same resolution with that of NCAM-LAMP was also used. The performance of the localized model in estimating forest water use was verified by comparison with the multi-year sapflow measurements and Eddy covariance data of Taehwa Mountain site. Our result can be used as referential information to estimate the forest water use change by the climate change. Moreover, the drought index can be used to foresee the drought condition and prepare to it.

A preliminary neutron diffraction study of rasburicase, a recombinant urate oxidase enzyme, complexed with 8-azaxanthin

DOE Office of Scientific and Technical Information (OSTI.GOV)

Budayova-Spano, Monika, E-mail: spano@embl-grenoble.fr; Institut Laue-Langevin, 6 Rue Jules Horowitz, BP 156, 38042 Grenoble; Bonneté, Françoise

2006-03-01

Neutron diffraction data of hydrogenated recombinant urate oxidase enzyme (Rasburicase), complexed with a purine-type inhibitor 8-azaxanthin, was collected to 2.1 Å resolution from a crystal grown in D{sub 2}O by careful control and optimization of crystallization conditions via knowledge of the phase diagram. Deuterium atoms were clearly seen in the neutron-scattering density map. Crystallization and preliminary neutron diffraction measurements of rasburicase, a recombinant urate oxidase enzyme expressed by a genetically modified Saccharomyces cerevisiae strain, complexed with a purine-type inhibitor (8-azaxanthin) are reported. Neutron Laue diffraction data were collected to 2.1 Å resolution using the LADI instrument from a crystal (grownmore » in D{sub 2}O) with volume 1.8 mm{sup 3}. The aim of this neutron diffraction study is to determine the protonation states of the inhibitor and residues within the active site. This will lead to improved comprehension of the enzymatic mechanism of this important enzyme, which is used as a protein drug to reduce toxic uric acid accumulation during chemotherapy. This paper illustrates the high quality of the neutron diffraction data collected, which are suitable for high-resolution structural analysis. In comparison with other neutron protein crystallography studies to date in which a hydrogenated protein has been used, the volume of the crystal was relatively small and yet the data still extend to high resolution. Furthermore, urate oxidase has one of the largest primitive unit-cell volumes (space group I222, unit-cell parameters a = 80, b = 96, c = 106 Å) and molecular weights (135 kDa for the homotetramer) so far successfully studied with neutrons.« less

PHOENIX IR Spectra of CO in the Sun and the Stars

NASA Astrophysics Data System (ADS)

Ayres, T. R.; Valenti, J. A.; Hinkle, K. H.; Johns-Krull, C. M.; Wiedemann, G. R.

1998-05-01

We report high-resolution (R ~ 5*E(4) ) spectra of the 2143 cm(-1) (4.7 mu m) interval---containing lines from the fundamental (Delta v =1) bands of carbon monoxide---in the Sun and other late-type stars, obtained with the PHOENIX cryogenic infrared spectrometer. The solar work was conducted at the McMath-Pierce telescope during the period 21--26 April 1997, while the stellar observations were obtained on the night of 6 December 1997 at the Kitt Peak 2.1-m. Comparisons of spatially-averaged spectra from the long-slit observations of the Sun with very high-resolution Fourier transform spectrometer scans permitted an evalution of the PHOENIX instrumental profile (affected by flexing of the grating owing to unequal thermal coefficients of the epoxy replica and the silicon substrate). The profile information subsequently was applied in comparisons of the stellar data sets with CO spectra synthesized using a variety of prototype thermal structure models. On the stellar side, we concentrated on bright K-type giants whose broad CO profiles are fully resolved at PHOENIX resolution. Our intent was to test the degree of thermal heterogeneity in the outer layers of the red giant atmospheres; analogous to the ``thermal bifurcation'' effects deduced in the solar context (namely, the dichotomy between classical hot chromosphere and the controversial cool ``COmosphere''). Our spectral analyses provide a preview of the power of PHOENIX for high-resolution infrared spectroscopy of stars; to be realized in the coming months when the original grating is replaced with an improved version. [-2mm] The observations were obtained at the National Optical Astronomy Observatories, which is operated by AURA, Inc., under a cooperative agreement with the National Science Foundation. This work was supported by NSF grant AST-9618505.

Using a flat-panel detector in high resolution cone beam CT for dental imaging.

PubMed

Baba, R; Ueda, K; Okabe, M

2004-09-01

Cone beam CT (CBCT) requires a two-dimensional X-ray detector. In the several CBCT systems developed for dental imaging, detection has been by the combination of an X-ray image intensifier and charge-coupled device (CCD) camera. In this paper, we propose a new CBCT system in which the detector is of the flat-panel type and evaluate its performance in dental imaging. We developed a prototype CBCT that has a flat-panel-type detector. The detector consists of a CsI scintillator screen and a photosensor array. First, the flat panel detector and image intensifier detector were compared in terms of the signal-to-noise ratio (SNR) of projected images. We then used these data and a theoretical formula to evaluate noise in reconstructed images. Second, reconstructed images of a bar pattern phantom were obtained as a way of evaluating the spatial resolution. Then, reconstructed images of a skull phantom were obtained. The SNR of the developed system was 1.6 times as high as that of a system with an image intensifier detector of equal detector pitch. The system was capable of resolving a 0.35 mm pattern and its field of view almost completely encompassed that of an image intensifier detector which is used in dentomaxillofacial imaging. The fine spatial resolution of the detector led to images in which the structural details of a skull phantom were clearly visible. The system's isotropically fine resolution will lead to improved precision in dental diagnosis and surgery. The next stage of our research will be the development of a flat panel detector system with a high frame acquisition rate.

Measurement of high-resolution mechanical contraction of cardiac muscle by induced eddy current.

PubMed

Lee, Young-Jae; Lee, Kang-Hwi; Kang, Seung-Jin; Kim, Kyeung-Nam; Khang, Seonah; Koo, Hye Ran; Gi, Sunok; Lee, Joo Hyeon; Lee, Jeong-Whan

2014-01-01

There are many types of devices which help to manage a personal health conditions such as heartbeat chest belt, pedometer and smart watch. And the most common device has the relationship with heart rate or ECG data. However, users have to attach some electrode or fasten the belt on the bare skin to measure bio-signal information. Therefore, most of people want more convenient and short-ready-time and no-need to attach electrode. In this paper, we proposed the high-resolution measuring system of mechanical activity of cardiac muscle and thereby measure heartbeat. The principle of the proposed measuring method is that the alternating current generate alternating magnetic field around coil. This primary magnetic field induces eddy current which makes magnetic field against primary coil in the nearby objects. To measure high-resolution changes of the induced secondary magnetic fields, we used digital Phase-locked loop(PLL) circuit which provides more high-resolution traces of frequency changes than the previous studies based on digital frequency counter method. As a result of our preliminary experiment, peak-peak intervals of the proposed method showed high correlation with R-R intervals of clinical ECG signals(r=0.9249). Also, from signal traces of the proposed method, we might make a conjecture that the contraction of atrium or ventricle is reflected by changing conductivity of cardiac muscle which is beating ceaselessly.

Development of a Dual-PIV system for high-speed flow applications

NASA Astrophysics Data System (ADS)

Schreyer, Anne-Marie; Lasserre, Jean J.; Dupont, Pierre

2015-10-01

A new Dual-particle image velocimetry (Dual-PIV) system for application in supersonic flows was developed. The system was designed for shock wave/turbulent boundary layer interactions with separation. This type of flow places demanding requirements on the system, from the large range of characteristic frequencies O(100 Hz-100 kHz) to spatial and temporal resolutions necessary for the measurement of turbulent quantities (Dolling in AIAA J 39(8):1517-1531, 2001; Dupont et al. in J Fluid Mech 559:255-277, 2006; Smits and Dussauge in Turbulent shear layers in supersonic flow, 2nd edn. Springer, New York, 2006). While classic PIV systems using high-resolution CCD sensors allow high spatial resolution, these systems cannot provide the required temporal resolution. Existing high-speed PIV systems provide temporal and CMOS sensor resolutions, and even laser pulse energies, that are not adapted to our needs. The only obvious solution allowing sufficiently high spatial resolution, access to high frequencies, and a high laser pulse energy is a multi-frame system: a Dual-PIV system, consisting of two synchronized PIV systems observing the same field of view, will give access to temporal characteristics of the flow. The key technology of our system is frequency-based image separation: two lasers of different wavelengths illuminate the field of view. The cross-pollution with laser light from the respective other branches was quantified during system validation. The overall system noise was quantified, and the prevailing error of only 2 % reflects the good spatial and temporal alignment. The quality of the measurement system is demonstrated with some results on a subsonic jet flow including the spatio-temporal inter-correlation functions between the systems. First measurements in a turbulent flat-plate boundary layer at Mach 2 show the same satisfactory data quality and are also presented and discussed.

High-resolution X-ray diffraction with no sample preparation

PubMed Central

Turner, S. M. R.; Degryse, P.; Shortland, A. J.

2017-01-01

It is shown that energy-dispersive X-ray diffraction (EDXRD) implemented in a back-reflection geometry is extremely insensitive to sample morphology and positioning even in a high-resolution configuration. This technique allows high-quality X-ray diffraction analysis of samples that have not been prepared and is therefore completely non-destructive. The experimental technique was implemented on beamline B18 at the Diamond Light Source synchrotron in Oxfordshire, UK. The majority of the experiments in this study were performed with pre-characterized geological materials in order to elucidate the characteristics of this novel technique and to develop the analysis methods. Results are presented that demonstrate phase identification, the derivation of precise unit-cell parameters and extraction of microstructural information on unprepared rock samples and other sample types. A particular highlight was the identification of a specific polytype of a muscovite in an unprepared mica schist sample, avoiding the time-consuming and difficult preparation steps normally required to make this type of identification. The technique was also demonstrated in application to a small number of fossil and archaeological samples. Back-reflection EDXRD implemented in a high-resolution configuration shows great potential in the crystallographic analysis of cultural heritage artefacts for the purposes of scientific research such as provenancing, as well as contributing to the formulation of conservation strategies. Possibilities for moving the technique from the synchrotron into museums are discussed. The avoidance of the need to extract samples from high-value and rare objects is a highly significant advantage, applicable also in other potential research areas such as palaeontology, and the study of meteorites and planetary materials brought to Earth by sample-return missions. PMID:28660862

Novel autosomal dominant mandibulofacial dysostosis with ptosis: clinical description and exclusion of TCOF1.

PubMed

Hedera, P; Toriello, H V; Petty, E M

2002-07-01

Treacher Collins syndrome (TCS), the most common type of mandibulofacial dysostosis (MFD), is genetically homogeneous. Other types of MFD are less common and, of these, only the Bauru type of MFD has an autosomal dominant (AD) mode of inheritance established. Here we report clinical features of a kindred with a unique AD MFD with the exclusion of linkage to the TCS locus (TCOF1) on chromosome 5q31-q32. Six affected family members underwent a complete medical genetics physical examination and two affected subjects had skeletal survey. All available medical records were reviewed. Linkage analysis using the markers spanning the TCOF1 locus was performed. One typically affected family member had a high resolution karyotype. Affected subjects had significant craniofacial abnormalities without any significant acral changes and thus had a phenotype consistent with a MFD variant. Distinctive features included hypoplasia of the zygomatic complex, micrognathia with malocclusion, auricular abnormalities with conductive hearing loss, and ptosis. Significantly negative two point lod scores were obtained for markers spanning the TCOF1 locus, excluding the possibility that the disease in our kindred is allelic with TCS. High resolution karyotype was normal. We report a kindred with a novel type of MFD that is not linked to the TCOF1 locus and is also clinically distinct from other types of AD MFD. Identification of additional families will facilitate identification of the gene causing this type of AD MFD and further characterisation of the clinical phenotype.

Characterizing bidirectional reflectance and spectral albedo of various land cover types in Midwest using GeoTASO Summer-2014 campaign

NASA Astrophysics Data System (ADS)

Wulamu, A.; Fishman, J.; Maimaitiyiming, M.; Leitch, J. W.; Zoogman, P.; Liu, X.; Chance, K.; Marshall, B.

2015-12-01

Understanding the bi-directional reflectance function (BRDF) and spectral albedo of various land-cover types is critical for retrieval of trace gas measurements from planned geostationary satellites such as the Tropospheric Emissions: Monitoring of Pollution (TEMPO). Radiant energy, which will be measured by these instruments at the top of atmosphere (TOA) at unprecedented spectral resolution, is strongly influenced by how this energy is reflected by the underlying surface. Thus, it is critical that we understand this phenomenon at comparable wavelength resolution. As part of the NASA ESTO-funded Geostationary Trace gas and Aerosol Sensor Optimization (GeoTASO) development project, we carried out synchronous field and airborne data collection campaigns in the St Louis Metro region in Summer 2014. We collected spectral reflectance data of various land cover types on the ground within hours of a GeoTASO overpass using a field-based hyperspectral spectroradiometer (model PSR3500 from Spectral Evolution). Field measurements collecting in-situ spectral albedo and bidirectional reflectance factors were also obtained in July and August of 2015. In this study, we present our preliminary findings from in-situ and airborne GeoTASO derived spectral albedo and BRDF characteristics of major land cover types at TEMPO spectral profiles, which are necessary for the accurate retrieval of tropospheric trace gases and aerosols. First, a spectral database of various targets (e.g., plants, soils, rocks, man-made objects and water) was developed using field measurements. Next, the GeoTASO airborne data were corrected using MODTRAN and field measurements to derive spectral albedo and BRDF. High spatial resolution land-cover types were extracted using satellite images (e.g., Landsat, WorldView, IKONOS, etc.) at resolutions from 2 m - 30 m. Lastly, spectral albedo/BRDFs corresponding to various land cover types were analyzed using both field and GeoTASO measurements.

High-resolution scanning electron microscopy of frozen-hydrated cells.

PubMed

Walther, P; Chen, Y; Pech, L L; Pawley, J B

1992-11-01

Cryo-fixed yeast Paramecia and sea urchin embryos were investigated with an in-lens type field-emission SEM using a cold stage. The goal was to further develop and investigate the processing of frozen samples for the low-temperature scanning electron microscope (LTSEM). Uncoated frozen-hydrated samples were imaged with the low-voltage backscattered electron signal (BSE). Resolution and contrast were sufficient to visualize cross-fractured membranes, nuclear pores and small vesicles in the cytoplasm. It is assumed that the resolution of this approach is limited by the extraction depth of the BSE which depends upon the accelerating voltage of the primary beam (V0). In this study, the lowest possible V0 was 2.6 kV because below this value the sensitivity of the BSE detector is insufficient. It is concluded that the resolution of the uncoated specimen could be improved if equipment were available for high-resolution BSE imaging at 0.5-2 kV. Higher resolution was obtained with platinum cryo-coated samples, on which intramembranous particles were easily imaged. These images even show the ring-like appearance of the hexagonally arranged intramembranous particles known from high-resolution replica studies. On fully hydrated samples at high magnification, the observation time for a particular area is limited by mass loss caused by electron irradiation. Other potential sources of artefacts are the deposition of water vapour contamination and shrinkage caused by the sublimation of ice. Imaging of partially dehydrated (partially freeze-dried) samples, e.g. high-pressure frozen Paramecium and sea urchin embryos, will probably become the main application in cell biology. In spite of possible shrinkage problems, this approach has a number of advantages compared with any other electron microscopy preparation method: no chemical fixation is necessary, eliminating this source of artefacts; due to partial removal of the water additional structures in the cytoplasm can be investigated; and finally, the mass loss due to electron beam irradiation is greatly reduced compared to fully frozen-hydrated specimens.

Resolution in forensic microbial genotyping

DOE Office of Scientific and Technical Information (OSTI.GOV)

Velsko, S P

2005-08-30

Resolution is a key parameter for differentiating among the large number of strain typing methods that could be applied to pathogens involved in bioterror events or biocrimes. In this report we develop a first-principles analysis of strain typing resolution using a simple mathematical model to provide a basis for the rational design of microbial typing systems for forensic applications. We derive two figures of merit that describe the resolving power and phylogenetic depth of a strain typing system. Rough estimates of these figures-of-merit for MLVA, MLST, IS element, AFLP, hybridization microarrays, and other bacterial typing methods are derived from mutationmore » rate data reported in the literature. We also discuss the general problem of how to construct a ''universal'' practical typing system that has the highest possible resolution short of whole-genome sequencing, and that is applicable with minimal modification to a wide range of pathogens.« less

Three-dimensional hydrogen microscopy using a high-energy proton probe

NASA Astrophysics Data System (ADS)

Dollinger, G.; Reichart, P.; Datzmann, G.; Hauptner, A.; Körner, H.-J.

2003-01-01

It is a challenge to measure two-dimensional or three-dimensional (3D) hydrogen profiles on a micrometer scale. Quantitative hydrogen analyses of micrometer resolution are demonstrated utilizing proton-proton scattering at a high-energy proton microprobe. It has more than an-order-of-magnitude better position resolution and in addition higher sensitivity than any other technique for 3D hydrogen analyses. This type of hydrogen imaging opens plenty room to characterize microstructured materials, and semiconductor devices or objects in microbiology. The first hydrogen image obtained with a 10 MeV proton microprobe shows the hydrogen distribution of the microcapillary system being present in the wing of a mayfly and demonstrates the potential of the method.

Using Airborne High Spectral Resolution Lidar Data to Evaluate Combined Active Plus Passive Retrievals of Aerosol Extinction Profiles

NASA Technical Reports Server (NTRS)

Burton, S. P.; Ferrare, R. A.; Kittaka, C.; Hostetler, C. A.; Hair, J. W.; Obland, M. D.; Rogers, R. R.; Cook, A. L.; Haper, D. B.

2008-01-01

Aerosol extinction profiles are derived from backscatter data by constraining the retrieval with column aerosol optical thickness (AOT), for example from coincident MODIS observations and without reliance on a priori assumptions about aerosol type or optical properties. The backscatter data were acquired with the NASA Langley High Spectral Resolution Lidar (HSRL). The HSRL also simultaneously measures extinction independently, thereby providing an ideal data set for evaluating the constrained retrieval of extinction from backscatter. We will show constrained extinction retrievals using various sources of column AOT, and examine comparisons with the HSRL extinction measurements and with a similar retrieval using data from the CALIOP lidar on the CALIPSO satellite.

Users manual for coordinate generation code CRDSRA

NASA Technical Reports Server (NTRS)

Shamroth, S. J.

1985-01-01

Generation of a viable coordinate system represents an important component of an isolated airfoil Navier-Stokes calculation. The manual describes a computer code for generation of such a coordinate system. The coordinate system is a general nonorthogonal one in which high resolution normal to the airfoil is obtained in the vicinity of the airfoil surface, and high resolution along the airfoil surface is obtained in the vicinity of the airfoil leading edge. The method of generation is a constructive technique which leads to a C type coordinate grid. The method of construction as well as input and output definitions are contained herein. The computer code itself as well as a sample output is being submitted to COSMIC.

Unification of some advection schemes in two dimensions

NASA Technical Reports Server (NTRS)

Sidilkover, D.; Roe, P. L.

1995-01-01

The relationship between two approaches towards construction of genuinely two-dimensional upwind advection schemes is established. One of these approaches is of the control volume type applicable on structured cartesian meshes. It resulted in the compact high resolution schemes capable of maintaining second order accuracy in both homogeneous and inhomogeneous cases. Another one is the fluctuation splitting approach, which is well suited for triangular (and possibly) unstructured meshes. Understanding the relationship between these two approaches allows us to formulate here a new fluctuation splitting high resolution (i.e. possible use of artificial compression, while maintaining positivity property) scheme. This scheme is shown to be linearity preserving in inhomogeneous as well as homogeneous cases.

High resolution projection X-ray microscope equipped with fluorescent X-ray analyzer and its applications

NASA Astrophysics Data System (ADS)

Minami, K.; Saito, Y.; Kai, H.; Shirota, K.; Yada, K.

2009-09-01

We have newly developed an open type fine-focus X-ray tube "TX-510" to realize a spatial resolution of 50nm and to radiate low energy characteristic X-rays for giving high absorption contrast to images of microscopic organisms. The "TX-510" employs a ZrO/W(100) Schottky emitter and an "In-Lens Field Emission Gun". The key points of the improvements are (1) reduced spherical aberration coefficient of magnetic objective lens, (2) easy and accurate focusing, (3) newly designed astigmatism compensator, (4) segmented thin film target for interchanging the target materials by electron beam shift and (5) fluorescent X-ray analysis system.

Sub-Nanosecond Cinematography In Laser Fusion Research: Current Techniques And Applications At The Lawrence Livermore National Laboratory*

NASA Astrophysics Data System (ADS)

Coleman, Lamar W...

1985-02-01

Progress in laser fusion research has increased the need for detail and precision in the diagnosis of experiments. This has spawned the development and use of sophisticated sub-nanosecond resolution diavostic systems. These systems typically use ultrafast x-ray or optical streak caAleras in combination. with spatially imaging or spectrally dispersing elements. These instruments provide high resolution data essential for understanding the processes occurrilltg in the interaction. of high. intensity laser light with targets. Several of these types of instruments and their capabilities will be discussed. The utilization of these kinds of diagnostics systems on the nearly completed 100 kJ Nova laser facility will be described.

Sub-nanosecond cinematography in laser fusion research: Current techniques and applications at the Lawrence Livermore Laboratory

NASA Astrophysics Data System (ADS)

Coleman, L. W.

1985-01-01

Progress in laser fusion research has increased the need for detail and precision in the diagnosis of experiments. This has spawned the development and use of sophisticated sub-nanosecond resolution diagnostic systems. These systems typically use ultrafast X-ray or optical streak cameras in combination with spatially imaging or spectrally dispersing elements. These instruments provide high resolution data essential for understanding the processes occurring in the interaction of high intensity laser light with targets. Several of these types of instruments and their capabilities will be discussed. The utilization of these kinds of diagnostics systems on the nearly completed 100 kJ Nova laser facility will be described.

Proteomic screening of glutamatergic mouse brain synaptosomes isolated by fluorescence activated sorting

PubMed Central

Biesemann, Christoph; Grønborg, Mads; Luquet, Elisa; Wichert, Sven P; Bernard, Véronique; Bungers, Simon R; Cooper, Ben; Varoqueaux, Frédérique; Li, Liyi; Byrne, Jennifer A; Urlaub, Henning; Jahn, Olaf; Brose, Nils; Herzog, Etienne

2014-01-01

For decades, neuroscientists have used enriched preparations of synaptic particles called synaptosomes to study synapse function. However, the interpretation of corresponding data is problematic as synaptosome preparations contain multiple types of synapses and non-synaptic neuronal and glial contaminants. We established a novel Fluorescence Activated Synaptosome Sorting (FASS) method that substantially improves conventional synaptosome enrichment protocols and enables high-resolution biochemical analyses of specific synapse subpopulations. Employing knock-in mice with fluorescent glutamatergic synapses, we show that FASS isolates intact ultrapure synaptosomes composed of a resealed presynaptic terminal and a postsynaptic density as assessed by light and electron microscopy. FASS synaptosomes contain bona fide glutamatergic synapse proteins but are almost devoid of other synapse types and extrasynaptic or glial contaminants. We identified 163 enriched proteins in FASS samples, of which FXYD6 and Tpd52 were validated as new synaptic proteins. FASS purification thus enables high-resolution biochemical analyses of specific synapse subpopulations in health and disease. PMID:24413018

What Do High-Resolution EIT Waves Tell Us About CMEs?

NASA Technical Reports Server (NTRS)

Thompson, Barbara

2010-01-01

Although many studies have demonstrated that some coronal waves are not generated by corona) mass ejections, we have learned a great deal about the ability of corona) mass ejections to drive large-scale corona) waves, also called "EIT waves." We present new results based on EIT wave amplitude, timing, speed, and direction of propagation, with respect to their correlation with CME-related dimmings, speeds, locations and widths. Furthermore, we demonstrate the ability to correlate different aspects of EIT waves with some of the observed structure of CMEs observed in coronagraph data. Finally, we expand on the discussion of the types of wave modes that can be generated by a corona) mass ejection, and how these observations can serve as a diagnostic of the type of impulse a CME can deliver to the surrounding corona. These diagnostics are obtained by examining the motion of individual field lines, requiring high-resolution observations like those provided by TRACE and SDO/AIA.

Urban Shanty Town Recognition Based on High-Resolution Remote Sensing Images and National Geographical Monitoring Features - a Case Study of Nanning City

NASA Astrophysics Data System (ADS)

He, Y.; He, Y.

2018-04-01

Urban shanty towns are communities that has contiguous old and dilapidated houses with more than 2000 square meters built-up area or more than 50 households. This study makes attempts to extract shanty towns in Nanning City using the product of Census and TripleSat satellite images. With 0.8-meter high-resolution remote sensing images, five texture characteristics (energy, contrast, maximum probability, and inverse difference moment) of shanty towns are trained and analyzed through GLCM. In this study, samples of shanty town are well classified with 98.2 % producer accuracy of unsupervised classification and 73.2 % supervised classification correctness. Low-rise and mid-rise residential blocks in Nanning City are classified into 4 different types by using k-means clustering and nearest neighbour classification respectively. This study initially establish texture feature descriptions of different types of residential areas, especially low-rise and mid-rise buildings, which would help city administrator evaluate residential blocks and reconstruction shanty towns.

Genotyping of Listeria monocytogenes isolates from poultry carcasses using high resolution melting (HRM) analysis.

PubMed

Sakaridis, Ioannis; Ganopoulos, Ioannis; Madesis, Panagiotis; Tsaftaris, Athanasios; Argiriou, Anagnostis

2014-01-02

An outbreak situation of human listeriosis requires a fast and accurate protocol for typing Listeria monocytogenes . Existing techniques are either characterized by low discriminatory power or are laborious and require several days to give a final result. Polymerase chain reaction (PCR) coupled with high resolution melting (HRM) analysis was investigated in this study as an alternative tool for a rapid and precise genotyping of L. monocytogenes isolates. Fifty-five isolates of L. monocytogenes isolated from poultry carcasses and the environment of four slaughterhouses were typed by HRM analysis using two specific markers, internalin B and ssrA genes. The analysis of genotype confidence percentage of L. monocytogenes isolates produced by HRM analysis generated dendrograms with two major groups and several subgroups. Furthermore, the analysis of the HRM curves revealed that all L. monocytogenes isolates could easily be distinguished. In conclusion, HRM was proven to be a fast and powerful tool for genotyping isolates of L. monocytogenes .

The ALMA correlator

NASA Astrophysics Data System (ADS)

Escoffier, R. P.; Comoretto, G.; Webber, J. C.; Baudry, A.; Broadwell, C. M.; Greenberg, J. H.; Treacy, R. R.; Cais, P.; Quertier, B.; Camino, P.; Bos, A.; Gunst, A. W.

2007-02-01

Aims: The Atacama Large Millimeter Array (ALMA) is an international astronomy facility to be used for detecting and imaging all types of astronomical sources at millimeter and submillimeter wavelengths at a 5000-m elevation site in the Atacama Desert of Chile. Our main aims are: describe the correlator sub-system which is that part of the ALMA system that combines the signal from up to 64 remote individual radio antennas and forms them into a single instrument; emphasize the high spectral resolution and the configuration flexibility available with the ALMA correlator. Methods: The main digital signal processing features and a block diagram of the correlator being constructed for the ALMA radio astronomy observatory are presented. Tables of observing modes and spectral resolutions offered by the correlator system are given together with some examples of multi-resolution spectral modes. Results: The correlator is delivered by quadrants and the first quadrant is being tested while most of the other printed circuit cards required by the system have been produced. In its final version the ALMA correlator will process the outputs of up to 64 antennas using an instantaneous bandwidth of 8 GHz in each of two polarizations per antenna. In the frequency division mode, unrivalled spectral flexibility together with very high resolution (3.8 kHz) and up to 8192 spectral points are achieved. In the time division mode high time resolution is available with minimum data dump rates of 16 ms for all cross-products.

High Resolution Ultrasound Superharmonic Perfusion Imaging: In Vivo Feasibility and Quantification of Dynamic Contrast-Enhanced Acoustic Angiography.

PubMed

Lindsey, Brooks D; Shelton, Sarah E; Martin, K Heath; Ozgun, Kathryn A; Rojas, Juan D; Foster, F Stuart; Dayton, Paul A

2017-04-01

Mapping blood perfusion quantitatively allows localization of abnormal physiology and can improve understanding of disease progression. Dynamic contrast-enhanced ultrasound is a low-cost, real-time technique for imaging perfusion dynamics with microbubble contrast agents. Previously, we have demonstrated another contrast agent-specific ultrasound imaging technique, acoustic angiography, which forms static anatomical images of the superharmonic signal produced by microbubbles. In this work, we seek to determine whether acoustic angiography can be utilized for high resolution perfusion imaging in vivo by examining the effect of acquisition rate on superharmonic imaging at low flow rates and demonstrating the feasibility of dynamic contrast-enhanced superharmonic perfusion imaging for the first time. Results in the chorioallantoic membrane model indicate that frame rate and frame averaging do not affect the measured diameter of individual vessels observed, but that frame rate does influence the detection of vessels near and below the resolution limit. The highest number of resolvable vessels was observed at an intermediate frame rate of 3 Hz using a mechanically-steered prototype transducer. We also demonstrate the feasibility of quantitatively mapping perfusion rate in 2D in a mouse model with spatial resolution of ~100 μm. This type of imaging could provide non-invasive, high resolution quantification of microvascular function at penetration depths of several centimeters.

Comparison and Evaluation of the Molecular Typing Methods for Toxigenic Vibrio cholerae in Southwest China.

PubMed

Liao, Feng; Mo, Zhishuo; Chen, Meiling; Pang, Bo; Fu, Xiaoqing; Xu, Wen; Jing, Huaiqi; Kan, Biao; Gu, Wenpeng

2018-01-01

Vibrio cholerae O1 strains taken from the repository of Yunnan province, southwest China, were abundant and special. We selected 70 typical toxigenic V. cholerae (69 O1 and one O139 serogroup strains) isolated from Yunnan province, performed the pulsed field gel electrophoresis (PFGE), multilocus sequence typing (MLST), and MLST of virulence gene (V-MLST) methods, and evaluated the resolution abilities for typing methods. The ctxB subunit sequence analysis for all strains have shown that cholera between 1986 and 1995 was associated with mixed infections with El Tor and El Tor variants, while infections after 1996 were all caused by El Tor variant strains. Seventy V. cholerae obtained 50 PFGE patterns, with a high resolution. The strains could be divided into three groups with predominance of strains isolated during 1980s, 1990s, and 2000s, respectively, showing a good consistency with the epidemiological investigation. We also evaluated two MLST method for V. cholerae , one was used seven housekeeping genes ( adk , gyrB , metE , pntA , mdh , purM , and pyrC ), and all the isolates belonged to ST69; another was used nine housekeeping genes ( cat , chi , dnaE , gyrB , lap , pgm , recA , rstA , and gmd ). A total of seven sequence types (STs) were found by using this method for all the strains; among them, rstA gene had five alleles, recA and gmd have two alleles, and others had only one allele. The virulence gene sequence typing method ( ctxAB , tcpA , and toxR ) showed that 70 strains were divided into nine STs; among them, tcpA gene had six alleles, toxR had five alleles, while ctxAB was identical for all the strains. The latter two sequences based typing methods also had consistency with epidemiology of the strains. PFGE had a higher resolution ability compared with the sequence based typing method, and MLST used seven housekeeping genes showed the lower resolution power than nine housekeeping genes and virulence genes methods. These two sequence typing methods could distinguish some epidemiological special strains in local area.

The type material of Mantodea (praying mantises) deposited in the National Museum of Natural History, Smithsonian Institution, USA

PubMed Central

Svenson, Gavin J.

2014-01-01

Abstract The collection of Mantodea of the National Museum of Natural History, Smithsonian Institution, includes 26 holotypes, 7 allotypes, 4 lectotypes, 23 paratypes, and 1 paralectotype. Four type specimens were designated as lectotypes within this work. Highly accurate measurement data, high resolution images of specimens and labels, verbatim label data, georeferenced coordinates, original and newly assigned database codes, and bibliographic data are presented for all primary types. Label data for all paratype specimens in the collection are provide in tabular form. The location of the USNM collection has been moved to the Cleveland Museum of Natural History as a loan under the Off-site Enhancement Program. PMID:25152673

Super-Resolution in Plenoptic Cameras Using FPGAs

PubMed Central

Pérez, Joel; Magdaleno, Eduardo; Pérez, Fernando; Rodríguez, Manuel; Hernández, David; Corrales, Jaime

2014-01-01

Plenoptic cameras are a new type of sensor that extend the possibilities of current commercial cameras allowing 3D refocusing or the capture of 3D depths. One of the limitations of plenoptic cameras is their limited spatial resolution. In this paper we describe a fast, specialized hardware implementation of a super-resolution algorithm for plenoptic cameras. The algorithm has been designed for field programmable graphic array (FPGA) devices using VHDL (very high speed integrated circuit (VHSIC) hardware description language). With this technology, we obtain an acceleration of several orders of magnitude using its extremely high-performance signal processing capability through parallelism and pipeline architecture. The system has been developed using generics of the VHDL language. This allows a very versatile and parameterizable system. The system user can easily modify parameters such as data width, number of microlenses of the plenoptic camera, their size and shape, and the super-resolution factor. The speed of the algorithm in FPGA has been successfully compared with the execution using a conventional computer for several image sizes and different 3D refocusing planes. PMID:24841246

Super-resolution in plenoptic cameras using FPGAs.

PubMed

Pérez, Joel; Magdaleno, Eduardo; Pérez, Fernando; Rodríguez, Manuel; Hernández, David; Corrales, Jaime

2014-05-16

Plenoptic cameras are a new type of sensor that extend the possibilities of current commercial cameras allowing 3D refocusing or the capture of 3D depths. One of the limitations of plenoptic cameras is their limited spatial resolution. In this paper we describe a fast, specialized hardware implementation of a super-resolution algorithm for plenoptic cameras. The algorithm has been designed for field programmable graphic array (FPGA) devices using VHDL (very high speed integrated circuit (VHSIC) hardware description language). With this technology, we obtain an acceleration of several orders of magnitude using its extremely high-performance signal processing capability through parallelism and pipeline architecture. The system has been developed using generics of the VHDL language. This allows a very versatile and parameterizable system. The system user can easily modify parameters such as data width, number of microlenses of the plenoptic camera, their size and shape, and the super-resolution factor. The speed of the algorithm in FPGA has been successfully compared with the execution using a conventional computer for several image sizes and different 3D refocusing planes.

Structures observed on the spot radiance fields during the FIRE experiment

NASA Technical Reports Server (NTRS)

Seze, Genevieve; Smith, Leonard; Desbois, Michel

1990-01-01

Three Spot images taken during the FIRE experiment on stratocumulus are analyzed. From this high resolution data detailed observations of the true cloud radiance field may be made. The structure and inhomogeneity of these radiance fields hold important implications for the radiation budget, while the fine scale structure in radiance field provides information on cloud dynamics. Wieliki and Welsh, and Parker et al., have quantified the inhomogeneities of the cumulus clouds through a careful examination of the distribution of cloud (and hole) size as functions of an effective cloud diameter and radiance threshold. Cahalan (1988) has compared for different cloud types of (stratocumulus, fair weather cumulus, convective clouds in the ITCZ) the distributions of clouds (and holes) sizes, the relation between the size and the perimeter of these clouds (and holes), and examining the possibility of scale invariance. These results are extended from LANDSAT resolution (57 m and 30 m) to the Spot resolution (10 m) resolution in the case of boundary layer clouds. Particular emphasis is placed on the statistics of zones of high and low reflectivity as a function of a threshold reflectivity.

Structures and textures of the Murchison and Mighei carbonaceous chondrite matrices

NASA Technical Reports Server (NTRS)

Mackinnon, I. D. R.

1980-01-01

High-resolution transmission electron microscopy has confirmed earlier observations that the character of the Murchison and Mighei fine-grained matrices is complex in mineralogy and texture. Layer structure minerals occur as planar laths, rounded grains or subhedral grains, and range in size from less than 100 A to about 1 micrometer. Serpentine-type and brucite-type structures predominate in the CM matrices. The occurrence of Povlen chrysolite and a vein of disordered mixed-layer and brucite-type material cutting a large lizardite-type grain suggests that at least some of the matrix materials were formed by alteration of preexisting material.

Automatic Building Damage Detection Method Using High-Resolution Remote Sensing Images and 3d GIS Model

NASA Astrophysics Data System (ADS)

Tu, Jihui; Sui, Haigang; Feng, Wenqing; Song, Zhina

2016-06-01

In this paper, a novel approach of building damaged detection is proposed using high resolution remote sensing images and 3D GIS-Model data. Traditional building damage detection method considers to detect damaged building due to earthquake, but little attention has been paid to analyze various building damaged types(e.g., trivial damaged, severely damaged and totally collapsed.) Therefore, we want to detect the different building damaged type using 2D and 3D feature of scenes because the real world we live in is a 3D space. The proposed method generalizes that the image geometric correction method firstly corrects the post-disasters remote sensing image using the 3D GIS model or RPC parameters, then detects the different building damaged types using the change of the height and area between the pre- and post-disasters and the texture feature of post-disasters. The results, evaluated on a selected study site of the Beichuan earthquake ruins, Sichuan, show that this method is feasible and effective in building damage detection. It has also shown that the proposed method is easily applicable and well suited for rapid damage assessment after natural disasters.

High resolution absorption spectrum of CO2between 1750 and 2000 Å. 2. Rotational analysis of two parallel-type bands assigned to the lowest electronic transition 13B2←

NASA Astrophysics Data System (ADS)

Cossart-Magos, Claudina; Launay, Françoise; Parkin, James E.

The absorption spectrum of CO2 gas between 175 and 200 nm was photographed at high resolution some years ago. This very weak spectral region proved to be extremely rich in bands showing rotational fine structure. In Part 1 [C. Cossart-Magos, F. Launay, J. E. Parkin, Mol. Phys., 75, 835 (1992), nine perpendicular-type bands were assigned to the lowest singlet-singlet transition, 11A2 ← ν'3 (b2) vibration. Here, the parallel-type bands observed at 185.7 and 175.6 nm are assigned to the lowest triplet-singlet transition, 13B2 ← TMPH0629math005 ν'2 (a1) vibration. The assignment and the rotational and spin constant values obtained are discussed in relation to previous experimental data and ab initio calculation results on the lowest excited states of CO2. The actual role of the 13B2 state in CO2 photodissociation, O(3P)+CO(X1Σ+) recombination, and O(1D) emission quenching by CO(X) molecules is reviewed.

The frequency of HLA-B(∗)57:01 and the risk of abacavir hypersensitivity reactions in the majority population of Costa Rica.

PubMed

Arrieta-Bolaños, Esteban; Madrigal, J Alejandro; Marsh, Steven G E; Shaw, Bronwen E; Salazar-Sánchez, Lizbeth

2014-11-01

HLA-B(∗)57:01 is a well-known and cost-effective pharmacogenetic marker for abacavir hypersensitivity. As with other HLA alleles, there is widespread variation in its frequency across populations. The Costa Rica Central Valley Population (CCVP) is the major population in this country. The frequency of HLA-B(∗)57:01 in this population has not been described yet. Thus, our aim was to determine the frequency of this allele in the CCVP. 200 unrelated healthy volunteer donors born in the CCVP were typed. HLA-B(∗)57-positive samples identified by HLA intermediate resolution typing methods were further typed by SBT to high resolution. An HLA-B(∗)57:01 carrier frequency of 5.00% was determined in this sample. This frequency is relatively high in comparison to reports from other populations in Latin America. These results suggest that there is a considerable frequency of HLA-B(∗)57:01 in the CCVP and that pharmacogenetic testing for HIV+ patients who are going to receive abacavir-based treatment should be considered in this country. Copyright © 2014 American Society for Histocompatibility and Immunogenetics. Published by Elsevier Inc. All rights reserved.

Velocity map imaging using an in-vacuum pixel detector.

PubMed

Gademann, Georg; Huismans, Ymkje; Gijsbertsen, Arjan; Jungmann, Julia; Visschers, Jan; Vrakking, Marc J J

2009-10-01

The use of a new type in-vacuum pixel detector in velocity map imaging (VMI) is introduced. The Medipix2 and Timepix semiconductor pixel detectors (256 x 256 square pixels, 55 x 55 microm2) are well suited for charged particle detection. They offer high resolution, low noise, and high quantum efficiency. The Medipix2 chip allows double energy discrimination by offering a low and a high energy threshold. The Timepix detector allows to record the incidence time of a particle with a temporal resolution of 10 ns and a dynamic range of 160 micros. Results of the first time application of the Medipix2 detector to VMI are presented, investigating the quantum efficiency as well as the possibility to operate at increased background pressure in the vacuum chamber.

A new collimator for I-123-IMP SPECT imaging of the brain

DOE Office of Scientific and Technical Information (OSTI.GOV)

Oyamada, H.; Fukukita, H.; Tanaka, E.

1985-05-01

At present, commercially available I-123-IMP is contaminated with I-124 and its concentration on the assay date is said to be approximately 5%. Therefore, the application of medium energy parallel hole collimator (MEPC) used in many places for SPECT results in deterioration of the image quality. Recently, the authors have developed a new collimator for I-123-IMP SPECT imaging comprised of 4 slat type units; ultrahigh resolution (UHR), high resolution (HR), high sensitivity (HS), and ultrahigh sensitivity (UHS). The slit width/septum thickness in mm for UHR, HR, HS, and UHS are 0.9/0.5, 1.5/0.85, 3.2/1.5, and 5.2/2.0, respectively. In practice, either UHR ormore » HR is set to the detector (Shimadzu LFOV-E, modified type) together with either HS or UHS. The former is always set to the detector with the slit direction parallel to the rotation axis, and the latter is set with its slit direction at a right angle to the former. This is based on an idea that, upon sacrifice of resolution to some extent, sensitivity can be gained on the axial direction while the resolution on the transaxial slice will still be sufficiently preserved. Resolutions (transaxial direction/axial direction) in FWHM (mm) for each combination (UHR-HS, UHR-UHS, HR-HS, and HR-UHS) were 15.9/31.4, 15.9/36.5,23.2/33.3, and 23.9/40.7, respectively, whereas the resolution of MEPC was 28.7/29.5. On the other hand, relative sensitivities to MEPC were 0.57, 0.86, 0.80, and 1.16. The authors conclude that the combination of UHR and HS is best suited for clinical practice and, at present they are obtaining I-123-IMP SPECT images of good quality.« less

"One-Stop Shop": Free-Breathing Dynamic Contrast-Enhanced Magnetic Resonance Imaging of the Kidney Using Iterative Reconstruction and Continuous Golden-Angle Radial Sampling.

PubMed

Riffel, Philipp; Zoellner, Frank G; Budjan, Johannes; Grimm, Robert; Block, Tobias K; Schoenberg, Stefan O; Hausmann, Daniel

2016-11-01

The purpose of the present study was to evaluate a recently introduced technique for free-breathing dynamic contrast-enhanced renal magnetic resonance imaging (MRI) applying a combination of radial k-space sampling, parallel imaging, and compressed sensing. The technique allows retrospective reconstruction of 2 motion-suppressed sets of images from the same acquisition: one with lower temporal resolution but improved image quality for subjective image analysis, and one with high temporal resolution for quantitative perfusion analysis. In this study, 25 patients underwent a kidney examination, including a prototypical fat-suppressed, golden-angle radial stack-of-stars T1-weighted 3-dimensional spoiled gradient-echo examination (GRASP) performed after contrast agent administration during free breathing. Images were reconstructed at temporal resolutions of 55 spokes per frame (6.2 seconds) and 13 spokes per frame (1.5 seconds). The GRASP images were evaluated by 2 blinded radiologists. First, the reconstructions with low temporal resolution underwent subjective image analysis: the radiologists assessed the best arterial phase and the best renal phase and rated image quality score for each patient on a 5-point Likert-type scale.In addition, the diagnostic confidence was rated according to a 3-point Likert-type scale. Similarly, respiratory motion artifacts and streak artifacts were rated according to a 3-point Likert-type scale.Then, the reconstructions with high temporal resolution were analyzed with a voxel-by-voxel deconvolution approach to determine the renal plasma flow, and the results were compared with values reported in previous literature. Reader 1 and reader 2 rated the overall image quality score for the best arterial phase and the best renal phase with a median image quality score of 4 (good image quality) for both phases, respectively. A high diagnostic confidence (median score of 3) was observed. There were no respiratory motion artifacts in any of the patients. Streak artifacts were present in all of the patients, but did not compromise diagnostic image quality.The estimated renal plasma flow was slightly higher (295 ± 78 mL/100 mL per minute) than reported in previous MRI-based studies, but also closer to the physiologically expected value. Dynamic, motion-suppressed contrast-enhanced renal MRI can be performed in high diagnostic quality during free breathing using a combination of golden-angle radial sampling, parallel imaging, and compressed sensing. Both morphologic and quantitative functional information can be acquired within a single acquisition.

A depth-encoding PET detector that uses light sharing and single-ended readout with silicon photomultipliers

NASA Astrophysics Data System (ADS)

Kuang, Zhonghua; Yang, Qian; Wang, Xiaohui; Fu, Xin; Ren, Ning; Sang, Ziru; Wu, San; Zheng, Yunfei; Zhang, Xianming; Hu, Zhanli; Du, Junwei; Liang, Dong; Liu, Xin; Zheng, Hairong; Yang, Yongfeng

2018-02-01

Detectors with depth-encoding capability and good timing resolution are required to develop high-performance whole-body or total-body PET scanners. In this work, depth-encoding PET detectors that use light sharing between two discrete crystals and single-ended readout with silicon photomultipliers (SiPMs) were manufactured and evaluated. The detectors consisted of two unpolished 3  ×  3  ×  20 mm3 LYSO crystals with different coupling materials between them and were read out by Hamamatsu 3  ×  3 mm2 SiPMs with one-to-one coupling. The ratio of the energy of one SiPM to the total energy of two SiPMs was used to measure the depth of interaction (DOI). Detectors with different coupling materials in-between the crystals were measured in the singles mode in an effort to obtain detectors that can provide good DOI resolution. The DOI resolution and energy resolution of three types of detector were measured and the timing resolution was measured for the detector with the best DOI and energy resolution. The optimum detector, with 5 mm optical glue, a 9 mm triangular ESR and a 6 mm rectangular ESR in-between the unpolished crystals, provides a DOI resolution of 2.65 mm, an energy resolution of 10.0% and a timing resolution of 427 ps for events of E  >  400 keV. The detectors simultaneously provide good DOI and timing resolution, and show great promise for the development of high-performance whole-body and total-body PET scanners.

Study on High Resolution Membrane-Based Diffractive Optical Imaging on Geostationary Orbit

NASA Astrophysics Data System (ADS)

Jiao, J.; Wang, B.; Wang, C.; Zhang, Y.; Jin, J.; Liu, Z.; Su, Y.; Ruan, N.

2017-05-01

Diffractive optical imaging technology provides a new way to realize high resolution earth observation on geostationary orbit. There are a lot of benefits to use the membrane-based diffractive optical element in ultra-large aperture optical imaging system, including loose tolerance, light weight, easy folding and unfolding, which make it easy to realize high resolution earth observation on geostationary orbit. The implementation of this technology also faces some challenges, including the configuration of the diffractive primary lens, the development of high diffraction efficiency membrane-based diffractive optical elements, and the correction of the chromatic aberration of the diffractive optical elements. Aiming at the configuration of the diffractive primary lens, the "6+1" petal-type unfold scheme is proposed, which consider the compression ratio, the blocking rate and the development complexity. For high diffraction efficiency membrane-based diffractive optical element, a self-collimating method is proposed. The diffraction efficiency is more than 90 % of the theoretical value. For the chromatic aberration correction problem, an optimization method based on schupmann is proposed to make the imaging spectral bandwidth in visible light band reach 100 nm. The above conclusions have reference significance for the development of ultra-large aperture diffractive optical imaging system.

The ASTRO-H X-ray Observatory

NASA Astrophysics Data System (ADS)

Takahashi, Tadayuki; Mitsuda, Kazuhisa; Kelley, Richard; Aarts, Henri; Aharonian, Felix; Akamatsu, Hiroki; Akimoto, Fumie; Allen, Steve; Anabuki, Naohisa; Angelini, Lorella; Arnaud, Keith; Asai, Makoto; Audard, Marc; Awaki, Hisamitsu; Azzarello, Philipp; Baluta, Chris; Bamba, Aya; Bando, Nobutaka; Bautz, Mark; Blandford, Roger; Boyce, Kevin; Brown, Greg; Cackett, Ed; Chernyakova, Mara; Coppi, Paolo; Costantini, Elisa; de Plaa, Jelle; den Herder, Jan-Willem; DiPirro, Michael; Done, Chris; Dotani, Tadayasu; Doty, John; Ebisawa, Ken; Eckart, Megan; Enoto, Teruaki; Ezoe, Yuichiro; Fabian, Andrew; Ferrigno, Carlo; Foster, Adam; Fujimoto, Ryuichi; Fukazawa, Yasushi; Funk, Stefan; Furuzawa, Akihiro; Galeazzi, Massimiliano; Gallo, Luigi; Gandhi, Poshak; Gendreau, Keith; Gilmore, Kirk; Haas, Daniel; Haba, Yoshito; Hamaguchi, Kenji; Hatsukade, Isamu; Hayashi, Takayuki; Hayashida, Kiyoshi; Hiraga, Junko; Hirose, Kazuyuki; Hornschemeier, Ann; Hoshino, Akio; Hughes, John; Hwang, Una; Iizuka, Ryo; Inoue, Yoshiyuki; Ishibashi, Kazunori; Ishida, Manabu; Ishimura, Kosei; Ishisaki, Yoshitaka; Ito, Masayuki; Iwata, Naoko; Iyomoto, Naoko; Kaastra, Jelle; Kallman, Timothy; Kamae, Tuneyoshi; Kataoka, Jun; Katsuda, Satoru; Kawahara, Hajime; Kawaharada, Madoka; Kawai, Nobuyuki; Kawasaki, Shigeo; Khangaluyan, Dmitry; Kilbourne, Caroline; Kimura, Masashi; Kinugasa, Kenzo; Kitamoto, Shunji; Kitayama, Tetsu; Kohmura, Takayoshi; Kokubun, Motohide; Kosaka, Tatsuro; Koujelev, Alex; Koyama, Katsuji; Krimm, Hans; Kubota, Aya; Kunieda, Hideyo; LaMassa, Stephanie; Laurent, Philippe; Lebrun, Francois; Leutenegger, Maurice; Limousin, Olivier; Loewenstein, Michael; Long, Knox; Lumb, David; Madejski, Grzegorz; Maeda, Yoshitomo; Makishima, Kazuo; Marchand, Genevieve; Markevitch, Maxim; Matsumoto, Hironori; Matsushita, Kyoko; McCammon, Dan; McNamara, Brian; Miller, Jon; Miller, Eric; Mineshige, Shin; Minesugi, Kenji; Mitsuishi, Ikuyuki; Miyazawa, Takuya; Mizuno, Tsunefumi; Mori, Hideyuki; Mori, Koji; Mukai, Koji; Murakami, Toshio; Murakami, Hiroshi; Mushotzky, Richard; Nagano, Hosei; Nagino, Ryo; Nakagawa, Takao; Nakajima, Hiroshi; Nakamori, Takeshi; Nakazawa, Kazuhiro; Namba, Yoshiharu; Natsukari, Chikara; Nishioka, Yusuke; Nobukawa, Masayoshi; Nomachi, Masaharu; O'Dell, Steve; Odaka, Hirokazu; Ogawa, Hiroyuki; Ogawa, Mina; Ogi, Keiji; Ohashi, Takaya; Ohno, Masanori; Ohta, Masayuki; Okajima, Takashi; Okamoto, Atsushi; Okazaki, Tsuyoshi; Ota, Naomi; Ozaki, Masanobu; Paerels, Fritzs; Paltani, Stéphane; Parmar, Arvind; Petre, Robert; Pohl, Martin; Porter, F. Scott; Ramsey, Brian; Reis, Rubens; Reynolds, Christopher; Russell, Helen; Safi-Harb, Samar; Sakai, Shin-ichiro; Sameshima, Hiroaki; Sanders, Jeremy; Sato, Goro; Sato, Rie; Sato, Yohichi; Sato, Kosuke; Sawada, Makoto; Serlemitsos, Peter; Seta, Hiromi; Shibano, Yasuko; Shida, Maki; Shimada, Takanobu; Shinozaki, Keisuke; Shirron, Peter; Simionescu, Aurora; Simmons, Cynthia; Smith, Randall; Sneiderman, Gary; Soong, Yang; Stawarz, Lukasz; Sugawara, Yasuharu; Sugita, Hiroyuki; Sugita, Satoshi; Szymkowiak, Andrew; Tajima, Hiroyasu; Takahashi, Hiromitsu; Takeda, Shin-ichiro; Takei, Yoh; Tamagawa, Toru; Tamura, Takayuki; Tamura, Keisuke; Tanaka, Takaaki; Tanaka, Yasuo; Tashiro, Makoto; Tawara, Yuzuru; Terada, Yukikatsu; Terashima, Yuichi; Tombesi, Francesco; Tomida, Hiroshi; Tsuboi, Yohko; Tsujimoto, Masahiro; Tsunemi, Hiroshi; Tsuru, Takeshi; Uchida, Hiroyuki; Uchiyama, Yasunobu; Uchiyama, Hideki; Ueda, Yoshihiro; Ueno, Shiro; Uno, Shinichiro; Urry, Meg; Ursino, Eugenio; de Vries, Cor; Wada, Atsushi; Watanabe, Shin; Werner, Norbert; White, Nicholas; Yamada, Takahiro; Yamada, Shinya; Yamaguchi, Hiroya; Yamasaki, Noriko; Yamauchi, Shigeo; Yamauchi, Makoto; Yatsu, Yoichi; Yonetoku, Daisuke; Yoshida, Atsumasa; Yuasa, Takayuki

2012-09-01

The joint JAXA/NASA ASTRO-H mission is the sixth in a series of highly successful X-ray missions initiated by the Institute of Space and Astronautical Science (ISAS). ASTRO-H will investigate the physics of the highenergy universe via a suite of four instruments, covering a very wide energy range, from 0.3 keV to 600 keV. These instruments include a high-resolution, high-throughput spectrometer sensitive over 0.3-12 keV with high spectral resolution of ΔE ≦ 7 eV, enabled by a micro-calorimeter array located in the focal plane of thin-foil X-ray optics; hard X-ray imaging spectrometers covering 5-80 keV, located in the focal plane of multilayer-coated, focusing hard X-ray mirrors; a wide-field imaging spectrometer sensitive over 0.4-12 keV, with an X-ray CCD camera in the focal plane of a soft X-ray telescope; and a non-focusing Compton-camera type soft gamma-ray detector, sensitive in the 40-600 keV band. The simultaneous broad bandpass, coupled with high spectral resolution, will enable the pursuit of a wide variety of important science themes.

A Novel, Aqueous Surface Treatment To Thermally Stabilize High Resolution Positive Photoresist Images*

NASA Astrophysics Data System (ADS)

Grunwald, John J.; Spencer, Allen C.

1986-07-01

The paper describes a new approach to thermally stabilize the already imaged profile of high resolution positive photoresists such as ULTRAMAC" PR-914. ***XD-4000, an aqueous emulsion of a blend of fluorine-bearing compounds is spun on top of the developed, positive photoresist-imaged wafer, and baked. This allows the photoresist to withstand temperatures up to at least 175 deg. C. while essentially maintaining vertical edge profiles. Also, adverse effects of "outgassing" in harsh environments, ie., plasma and ion implant are greatly minimized by allowing the high resolution imaged photoresist to be post-baked at "elevated" temperatures. Another type of product that accomplishes the same effect is ***XD-4005, an aqueous emulsion of a high temperature-resistant polymer. While the exact mechanism is yet to be identified, it is postulated that absorption of the "polymeric" species into the "skin" of the imaged resist forms a temperature resistant "envelope", thereby allowing high resolution photoresists to also serve in a "high temperature" mode, without reticulation, or other adverse effects due to thermal degradation. SEM's are presented showing imaged ULTRAMAC" PR-914 and ULTRAMAC" **EPA-914 geometries coated with XD-4000 or XD-4005 and followed by plasma etched oxide,polysilicon and aluminum. Selectivity ratios are compared with and without the novel treatment and are shown to be significantly better with the treatment. The surface-treated photoresist for thermal resistance remains easily strippable in solvent-based or plasma media, unlike photoresists that have undergone "PRIST" or other gaseous thermal stabilization methods.

High Resolution Thz and FIR Spectroscopy of SOCl_2

NASA Astrophysics Data System (ADS)

Martin-Drumel, M. A.; Cuisset, A.; Sadovskii, D. A.; Mouret, G.; Hindle, F.; Pirali, O.

2013-06-01

Thionyl chloride (SOCl_2) is an extremely powerful oxidant widely used in industrial processes and playing a role in the chemistry of the atmosphere. In addition, it has a molecular configuration similar to that of phosgene (COCl_2), and is therefore of particular interest for security and defense applications. Low resolution vibrational spectra of gas phase SOCl_2 as well as high resolution pure rotational transitions up to 25 GHz have previously been investigated. To date no high resolution data are reported at frequencies higher than 25 GHz. We have investigated the THz absorption spectrum of SOCl_2 in the spectral region 70-650 GHz using a frequency multiplier chain coupled to a 1 m long single path cell containing a pressure of about 15 μbar. At the time of the writing, about 8000 pure rotational transitions of SO^{35}Cl_2 with highest J and K_a values of 110 and 50 respectively have been assigned on the spectrum. We have also recorded the high resolution FIR spectra of SOCl_2 in the spectral range 50-700 wn using synchrotron radiation at the AILES beamline of SOLEIL facility. A White-type cell aligned with an absorption path length of 150 m has been used to record, at a resolution of 0.001 wn, two spectra at pressures of 5 and 56 μbar of SOCl_2. On these spectra all FIR modes of SOCl_2 are observed (ν_2 to ν_6) and present a resolved rotational structure. Their analysis is in progress. T. J. Johnson et al., J. Phys. Chem. A 107, 6183 (2003) D. E. Martz and R. T. Lagemann, J. Chem. Phys. 22,1193 (1954) H. S. P. Müller and M. C. L. Gerry, J. Chem. Soc. Faraday Trans. 90, 3473 (1994)

Estimating Vegetation Rainfall Interception Using Remote Sensing Observations at Very High Resolution

NASA Astrophysics Data System (ADS)

Cui, Y.; Zhao, P.; Hong, Y.; Fan, W.; Yan, B.; Xie, H.

2017-12-01

Abstract: As an important compont of evapotranspiration, vegetation rainfall interception is the proportion of gross rainfall that is intercepted, stored and subsequently evaporated from all parts of vegetation during or following rainfall. Accurately quantifying the vegetation rainfall interception at a high resolution is critical for rainfall-runoff modeling and flood forecasting, and is also essential for understanding its further impact on local, regional, and even global water cycle dynamics. In this study, the Remote Sensing-based Gash model (RS-Gash model) is developed based on a modified Gash model for interception loss estimation using remote sensing observations at the regional scale, and has been applied and validated in the upper reach of the Heihe River Basin of China for different types of vegetation. To eliminate the scale error and the effect of mixed pixels, the RS-Gash model is applied at a fine scale of 30 m with the high resolution vegetation area index retrieved by using the unified model of bidirectional reflectance distribution function (BRDF-U) for the vegetation canopy. Field validation shows that the RMSE and R2 of the interception ratio are 3.7% and 0.9, respectively, indicating the model's strong stability and reliability at fine scale. The temporal variation of vegetation rainfall interception loss and its relationship with precipitation are further investigated. In summary, the RS-Gash model has demonstrated its effectiveness and reliability in estimating vegetation rainfall interception. When compared to the coarse resolution results, the application of this model at 30-m fine resolution is necessary to resolve the scaling issues as shown in this study. Keywords: rainfall interception; remote sensing; RS-Gash analytical model; high resolution

The Hemiptera type-material housed in the "Museu de Ciências Naturais, Fundação Zoobotânica do Rio Grande do Sul" of Porto Alegre, Brazil.

PubMed

Ruschel, Tatiana Petersen; Guidoti, Marcus; Barcellos, Aline

2013-01-01

We provide a commented and referenced list on the type material deposited in the "Museu de Ciencias Naturais, Fundação Zoobotânica do Rio Grande do Sul", Porto Alegre, Brazil. Geographic coordinates are available on a digital repository for free access. High-resolution images of the specimens are available under request.

Electro-mechanical coupling of semiconductor film grown on stainless steel by oxidation

NASA Astrophysics Data System (ADS)

Lin, M. C.; Wang, G.; Guo, L. Q.; Qiao, L. J.; Volinsky, Alex A.

2013-09-01

Electro-mechanical coupling phenomenon in oxidation film on stainless steel has been discovered by using current-sensing atomic force microscopy, along with the I-V curves measurements. The oxidation films exhibit either ohmic, n-type, or p-type semiconductor properties, according to the obtained I-V curves. This technique allows characterizing oxidation films with high spatial resolution. Semiconductor properties of oxidation films must be considered as additional stress corrosion cracking mechanisms.

A novel typing method for Listeria monocytogenes using high-resolution melting analysis (HRMA) of tandem repeat regions.

PubMed

Ohshima, Chihiro; Takahashi, Hajime; Iwakawa, Ai; Kuda, Takashi; Kimura, Bon

2017-07-17

Listeria monocytogenes, which is responsible for causing food poisoning known as listeriosis, infects humans and animals. Widely distributed in the environment, this bacterium is known to contaminate food products after being transmitted to factories via raw materials. To minimize the contamination of products by food pathogens, it is critical to identify and eliminate factory entry routes and pathways for the causative bacteria. High resolution melting analysis (HRMA) is a method that takes advantage of differences in DNA sequences and PCR product lengths that are reflected by the disassociation temperature. Through our research, we have developed a multiple locus variable-number tandem repeat analysis (MLVA) using HRMA as a simple and rapid method to differentiate L. monocytogenes isolates. While evaluating our developed method, the ability of MLVA-HRMA, MLVA using capillary electrophoresis, and multilocus sequence typing (MLST) was compared for their ability to discriminate between strains. The MLVA-HRMA method displayed greater discriminatory ability than MLST and MLVA using capillary electrophoresis, suggesting that the variation in the number of repeat units, along with mutations within the DNA sequence, was accurately reflected by the melting curve of HRMA. Rather than relying on DNA sequence analysis or high-resolution electrophoresis, the MLVA-HRMA method employs the same process as PCR until the analysis step, suggesting a combination of speed and simplicity. The result of MLVA-HRMA method is able to be shared between different laboratories. There are high expectations that this method will be adopted for regular inspections at food processing facilities in the near future. Copyright © 2017. Published by Elsevier B.V.

Application of High-Resolution Thermal Infrared Remote Sensing and GIS to Assess the Urban Heat Island Effect

NASA Technical Reports Server (NTRS)

Lo, C. P.; Quattrochi, D. A.; Luvall, J. C.

1997-01-01

Day and night airborne thermal infrared image data at 5 m spatial resolution acquired with the 15-channel (0.45 micron - 12.2 micron) Advanced Thermal and Land Applications Sensor (ATLAS) over Alabama, Huntsville on 7 September, 1994 were used to study changes in the thermal signatures of urban land cover types between day and night. Thermal channel number 13 (9.6 micron - 10.2 micron) data with the best noise-equivalent temperature change (NEAT) of 0.25 C after atmospheric corrections and temperature calibration were selected for use in this analysis. This research also examined the relation between land cover irradiance and vegetation amount, using the Normalized Difference Vegetation Index (NDVI), obtained by ratioing the difference and the sum of the red (channel number 3: 0.60-0.63 micron) and reflected infrared (channel number 6: 0.76-0.90 micron) ATLAS data. Based on the mean radiance values, standard deviations, and NDVI extracted from 351 pairs of polygons of day and night channel number 13 images for the city of Huntsville, a spatial model of warming and cooling characteristics of commercial, residential, agricultural, vegetation, and water features was developed using a GIS approach. There is a strong negative correlation between NDVI and irradiance of residential, agricultural, and vacant/transitional land cover types, indicating that the irradiance of a land cover type is greatly influenced by the amount of vegetation present. The predominance of forests, agricultural, and residential uses associated with varying degrees of tree cover showed great contrasts with commercial and services land cover types in the center of the city, and favors the development of urban heat islands. The high-resolution thermal infrared images match the complexity of the urban environment, and are capable of characterizing accurately the urban land cover types for the spatial modeling of the urban heat island effect using a GIS approach.

Object Based Image Analysis Combining High Spatial Resolution Imagery and Laser Point Clouds for Urban Land Cover

NASA Astrophysics Data System (ADS)

Zou, Xiaoliang; Zhao, Guihua; Li, Jonathan; Yang, Yuanxi; Fang, Yong

2016-06-01

With the rapid developments of the sensor technology, high spatial resolution imagery and airborne Lidar point clouds can be captured nowadays, which make classification, extraction, evaluation and analysis of a broad range of object features available. High resolution imagery, Lidar dataset and parcel map can be widely used for classification as information carriers. Therefore, refinement of objects classification is made possible for the urban land cover. The paper presents an approach to object based image analysis (OBIA) combing high spatial resolution imagery and airborne Lidar point clouds. The advanced workflow for urban land cover is designed with four components. Firstly, colour-infrared TrueOrtho photo and laser point clouds were pre-processed to derive the parcel map of water bodies and nDSM respectively. Secondly, image objects are created via multi-resolution image segmentation integrating scale parameter, the colour and shape properties with compactness criterion. Image can be subdivided into separate object regions. Thirdly, image objects classification is performed on the basis of segmentation and a rule set of knowledge decision tree. These objects imagery are classified into six classes such as water bodies, low vegetation/grass, tree, low building, high building and road. Finally, in order to assess the validity of the classification results for six classes, accuracy assessment is performed through comparing randomly distributed reference points of TrueOrtho imagery with the classification results, forming the confusion matrix and calculating overall accuracy and Kappa coefficient. The study area focuses on test site Vaihingen/Enz and a patch of test datasets comes from the benchmark of ISPRS WG III/4 test project. The classification results show higher overall accuracy for most types of urban land cover. Overall accuracy is 89.5% and Kappa coefficient equals to 0.865. The OBIA approach provides an effective and convenient way to combine high resolution imagery and Lidar ancillary data for classification of urban land cover.

Quarterly Performance/Technical Report of the National Marrow Donor Program

DTIC Science & Technology

2008-02-05

HLA-DRB1 High Resolution Typing Closed 9 IIB.1.3 Aim 3 – Evaluate HLA-C Typing of Donors Closed 9 IIB.1.4 Aim 4 – Evaluate Buccal Swabs Open 10 IIB...December 31, 2007 10 of 21 IIB.1.4 Aim 4: Evaluate Buccal Swabs Period 5 Activity: In April of 2006, the NMDP transitioned to the use of... buccal swabs for the collection of DNA samples from newly recruited donors. To support this change in sample type, Quality Control samples for donor

Investigation relative to the Roentgen Satellite (ROSAT)

NASA Technical Reports Server (NTRS)

Elvis, Martin S.; Primini, Francis A.; Fabbiano, Guiseppina; Harris, Daniel E.; Jones-Foreman, Christine; Trinchieri, Ginevra; Golub, Leon; Bookbinder, Jay; Seward, Frederick D.; Zombeck, Martin V.

1994-01-01

Reports include: High Resolution Observations of the Central Region of M31; The X-ray Emission of Low-X-ray-Luminosity Early-Type Galaxies: Gas Versus Compact Sources; Interaction Between Cluster Gas and Radio Features of Cygnus A; Hot Gas and Dark Halos in Early-Type Galaxies; A Gravitational Lens in X-rays - 0957+461; How Massive are Early-Type Galaxies?; Three Crab-Like SNR in the Large Magellanic Cloud; and Soft X-ray Emission from Boundary Layers in Cataclysmic Variables. Papers submitted to the Astrophysical Journal are attached.

The effect of spatial resolution on water scarcity estimates in Australia

NASA Astrophysics Data System (ADS)

Gevaert, Anouk; Veldkamp, Ted; van Dijk, Albert; Ward, Philip

2017-04-01

Water scarcity is an important global issue with severe socio-economic consequences, and its occurrence is likely to increase in many regions due to population growth, economic development and climate change. This has prompted a number of global and regional studies to identify areas that are vulnerable to water scarcity and to determine how this vulnerability will change in the future. A drawback of these studies, however, is that they typically have coarse spatial resolutions. Here, we studied the effect of increasing the spatial resolution of water scarcity estimates in Australia, and the Murray-Darling Basin in particular. This was achieved by calculating the water stress index (WSI), an indicator showing the ratio of water use to water availability, at 0.5 and 0.05 degree resolution for the period 1990-2010. Monthly water availability data were based on outputs of the Australian Water Resources Assessment Landscape model (AWRA-L), which was run at both spatial resolutions and at a daily time scale. Water use information was obtained from a monthly 0.5 degree global dataset that distinguishes between water consumption for irrigation, livestock, industrial and domestic uses. The data were downscaled to 0.05 degree by dividing the sectoral water uses over the areas covered by relevant land use types using a high resolution ( 0.5km) land use dataset. The monthly WSIs at high and low resolution were then used to evaluate differences in the patterns of water scarcity frequency and intensity. In this way, we assess to what extent increasing the spatial resolution can improve the identification of vulnerable areas and thereby assist in the development of strategies to lower this vulnerability. The results of this study provide insight into the scalability of water scarcity estimates and the added value of high resolution water scarcity information in water resources management.

Initial phantom study comparing image quality in computed tomography using adaptive statistical iterative reconstruction and new adaptive statistical iterative reconstruction v.

PubMed

Lim, Kyungjae; Kwon, Heejin; Cho, Jinhan; Oh, Jongyoung; Yoon, Seongkuk; Kang, Myungjin; Ha, Dongho; Lee, Jinhwa; Kang, Eunju

2015-01-01

The purpose of this study was to assess the image quality of a novel advanced iterative reconstruction (IR) method called as "adaptive statistical IR V" (ASIR-V) by comparing the image noise, contrast-to-noise ratio (CNR), and spatial resolution from those of filtered back projection (FBP) and adaptive statistical IR (ASIR) on computed tomography (CT) phantom image. We performed CT scans at 5 different tube currents (50, 70, 100, 150, and 200 mA) using 3 types of CT phantoms. Scanned images were subsequently reconstructed in 7 different scan settings, such as FBP, and 3 levels of ASIR and ASIR-V (30%, 50%, and 70%). The image noise was measured in the first study using body phantom. The CNR was measured in the second study using contrast phantom and the spatial resolutions were measured in the third study using a high-resolution phantom. We compared the image noise, CNR, and spatial resolution among the 7 reconstructed image scan settings to determine whether noise reduction, high CNR, and high spatial resolution could be achieved at ASIR-V. At quantitative analysis of the first and second studies, it showed that the images reconstructed using ASIR-V had reduced image noise and improved CNR compared with those of FBP and ASIR (P < 0.001). At qualitative analysis of the third study, it also showed that the images reconstructed using ASIR-V had significantly improved spatial resolution than those of FBP and ASIR (P < 0.001). Our phantom studies showed that ASIR-V provides a significant reduction in image noise and a significant improvement in CNR as well as spatial resolution. Therefore, this technique has the potential to reduce the radiation dose further without compromising image quality.

Mapping of West Siberian taiga wetland complexes using Landsat imagery: implications for methane emissions

NASA Astrophysics Data System (ADS)

Evgenievna Terentieva, Irina; Vladimirovich Glagolev, Mikhail; Dmitrievna Lapshina, Elena; Faritovich Sabrekov, Alexandr; Maksyutov, Shamil

2016-08-01

High-latitude wetlands are important for understanding climate change risks because these environments sink carbon dioxide and emit methane. However, fine-scale heterogeneity of wetland landscapes poses a serious challenge when generating regional-scale estimates of greenhouse gas fluxes from point observations. In order to reduce uncertainties at the regional scale, we mapped wetlands and water bodies in the taiga zone of The West Siberia Lowland (WSL) on a scene-by-scene basis using a supervised classification of Landsat imagery. Training data consist of high-resolution images and extensive field data collected at 28 test areas. The classification scheme aims at supporting methane inventory applications and includes seven wetland ecosystem types comprising nine wetland complexes distinguishable at the Landsat resolution. To merge typologies, mean relative areas of wetland ecosystems within each wetland complex type were estimated using high-resolution images. Accuracy assessment based on 1082 validation polygons of 10 × 10 pixel size indicated an overall map accuracy of 79 %. The total area of the WSL wetlands and water bodies was estimated to be 52.4 Mha or 4-12 % of the global wetland area. Ridge-hollow complexes prevail in WSL's taiga zone accounting for 33 % of the total wetland area, followed by pine bogs or "ryams" (23 %), ridge-hollow-lake complexes (16 %), open fens (8 %), palsa complexes (7 %), open bogs (5 %), patterned fens (4 %), and swamps (4 %). Various oligotrophic environments are dominant among wetland ecosystems, while poor fens cover only 14 % of the area. Because of the significant change in the wetland ecosystem coverage in comparison to previous studies, a considerable reevaluation of the total CH4 emissions from the entire region is expected. A new Landsat-based map of WSL's taiga wetlands provides a benchmark for validation of coarse-resolution global land cover products and wetland data sets in high latitudes.

High Resolution Digital Surface Model For Production Of Airport Obstruction Charts Using Spaceborne SAR Sensors

NASA Astrophysics Data System (ADS)

Oliveira, Henrique; Rodrigues, Marco; Radius, Andrea

2012-01-01

Airport Obstruction Charts (AOCs) are graphical representations of natural or man-made obstructions (its locations and heights) around airfields, according to International Civil Aviation Organization (ICAO) Annexes 4, 14 and 15. One of the most important types of data used in AOCs production/update tasks is a Digital Surface Model (first reflective surface) of the surveyed area. The development of advanced remote sensing technologies provide the available tools for obstruction data acquisition, while Geographic Information Systems (GIS) present the perfect platform for storing and analyzing this type of data, enabling the production of digital ACOs, greatly contributing to the increase of the situational awareness of pilots and enhancing the air navigation safety level [1]. Data acquisition corresponding to the first reflective surface can be obtained through the use of Airborne Laser-Scanning and Light Detection and Ranging (ALS/LIDAR) or Spaceborne SAR Systems. The need of surveying broad areas, like the entire territory of a state, shows that Spaceborne SAR systems are the most adequate in economic and feasibility terms of the process, to perform the monitoring and producing a high resolution Digital Surface Model (DSM). The high resolution DSM generation depends on many factors: the available data set, the used technique and the setting parameters. To increase the precision and obtain high resolution products, two techniques are available using a stack of data: the PS (Permanent Scatterers) technique [2], that uses large stack of data to identify many stable and coherent targets through multi- temporal analysis, removing the atmospheric contribution and to minimize the estimation errors, and the Small Baseline Subset (SBAS) technique ([3],[4]), that relies on the use of small baseline SAR interferograms and on the application of the so called singular value decomposition (SVD) method, in order to link independent SAR acquisition data sets, separated by large baselines, thus increasing the number of data used for the analysis.

Imaging the motion of electrons in 2D semiconductor heterostructures

NASA Astrophysics Data System (ADS)

Dani, Keshav

Technological progress since the late 20th century has centered on semiconductor devices, such as transistors, diodes, and solar cells. At the heart of these devices, is the internal motion of electrons through semiconductor materials due to applied electric fields or by the excitation of photocarriers. Imaging the motion of these electrons would provide unprecedented insight into this important phenomenon, but requires high spatial and temporal resolution. Current studies of electron dynamics in semiconductors are generally limited by the spatial resolution of optical probes, or by the temporal resolution of electronic probes. In this talk, we combine femtosecond pump-probe techniques with spectroscopic photoemission electron microscopy to image the motion of photoexcited electrons from high-energy to low-energy states in a 2D InSe/GaAs heterostructure exhibiting a type-II band alignment. At the instant of photoexcitation, energy-resolved photoelectron images reveal a highly non-equilibrium distribution of photocarriers in space and energy. Thereafter, in response to the out-of-equilibrium photocarriers, we observe the spatial redistribution of charges, thus forming internal electric fields, bending the semiconductor bands, and finally impeding further charge transfer. By assembling images taken at different time-delays, we make a movie lasting a few tens of picoseconds of the electron transfer process in the photoexcited type-II heterostructure - a fundamental phenomenon in semiconductor devices like solar cells. Quantitative analysis and theoretical modeling of spatial variations in the video provide insight into future solar cells, electron dynamics in 2D materials, and other semiconductor devices.

Imaging the motion of electrons across semiconductor heterojunctions.

PubMed

Man, Michael K L; Margiolakis, Athanasios; Deckoff-Jones, Skylar; Harada, Takaaki; Wong, E Laine; Krishna, M Bala Murali; Madéo, Julien; Winchester, Andrew; Lei, Sidong; Vajtai, Robert; Ajayan, Pulickel M; Dani, Keshav M

2017-01-01

Technological progress since the late twentieth century has centred on semiconductor devices, such as transistors, diodes and solar cells. At the heart of these devices is the internal motion of electrons through semiconductor materials due to applied electric fields or by the excitation of photocarriers. Imaging the motion of these electrons would provide unprecedented insight into this important phenomenon, but requires high spatial and temporal resolution. Current studies of electron dynamics in semiconductors are generally limited by the spatial resolution of optical probes, or by the temporal resolution of electronic probes. Here, by combining femtosecond pump-probe techniques with spectroscopic photoemission electron microscopy, we imaged the motion of photoexcited electrons from high-energy to low-energy states in a type-II 2D InSe/GaAs heterostructure. At the instant of photoexcitation, energy-resolved photoelectron images revealed a highly non-equilibrium distribution of photocarriers in space and energy. Thereafter, in response to the out-of-equilibrium photocarriers, we observed the spatial redistribution of charges, thus forming internal electric fields, bending the semiconductor bands, and finally impeding further charge transfer. By assembling images taken at different time-delays, we produced a movie lasting a few trillionths of a second of the electron-transfer process in the photoexcited type-II heterostructure-a fundamental phenomenon in semiconductor devices such as solar cells. Quantitative analysis and theoretical modelling of spatial variations in the movie provide insight into future solar cells, 2D materials and other semiconductor devices.

Imaging the motion of electrons across semiconductor heterojunctions

NASA Astrophysics Data System (ADS)

Man, Michael K. L.; Margiolakis, Athanasios; Deckoff-Jones, Skylar; Harada, Takaaki; Wong, E. Laine; Krishna, M. Bala Murali; Madéo, Julien; Winchester, Andrew; Lei, Sidong; Vajtai, Robert; Ajayan, Pulickel M.; Dani, Keshav M.

2017-01-01

Technological progress since the late twentieth century has centred on semiconductor devices, such as transistors, diodes and solar cells. At the heart of these devices is the internal motion of electrons through semiconductor materials due to applied electric fields or by the excitation of photocarriers. Imaging the motion of these electrons would provide unprecedented insight into this important phenomenon, but requires high spatial and temporal resolution. Current studies of electron dynamics in semiconductors are generally limited by the spatial resolution of optical probes, or by the temporal resolution of electronic probes. Here, by combining femtosecond pump-probe techniques with spectroscopic photoemission electron microscopy, we imaged the motion of photoexcited electrons from high-energy to low-energy states in a type-II 2D InSe/GaAs heterostructure. At the instant of photoexcitation, energy-resolved photoelectron images revealed a highly non-equilibrium distribution of photocarriers in space and energy. Thereafter, in response to the out-of-equilibrium photocarriers, we observed the spatial redistribution of charges, thus forming internal electric fields, bending the semiconductor bands, and finally impeding further charge transfer. By assembling images taken at different time-delays, we produced a movie lasting a few trillionths of a second of the electron-transfer process in the photoexcited type-II heterostructure—a fundamental phenomenon in semiconductor devices such as solar cells. Quantitative analysis and theoretical modelling of spatial variations in the movie provide insight into future solar cells, 2D materials and other semiconductor devices.

Improving Science Communication with Responsive Web Design

NASA Astrophysics Data System (ADS)

Hilverda, M.

2013-12-01

Effective science communication requires clarity in both content and presentation. Content is increasingly being viewed via the Web across a broad range of devices, which can vary in screen size, resolution, and pixel density. Readers access the same content from desktop computers, tablets, smartphones, and wearable computing devices. Creating separate presentation formats optimized for each device is inefficient and unrealistic as new devices continually enter the marketplace. Responsive web design is an approach that puts content first within a presentation design that responds automatically to its environment. This allows for one platform to be maintained that can be used effectively for every screen. The layout adapts to screens of all sizes ensuring easy viewing of content for readers regardless of their device. Responsive design is accomplished primarily by the use of media queries within style sheets, which allows for changes to layout properties to be defined based on media types (i.e. screen, print) and resolution. Images and other types of multimedia can also be defined to scale automatically to fit different screen dimensions, although some media types require additional effort for proper implementation. Hardware changes, such as high pixel density screens, also present new challenges for effective presentation of content. High pixel density screens contain a greater number of pixels within a screen area increasing the pixels per inch (PPI) compared to standard screens. The result is increased clarity for text and vector media types, but often decreased clarity for standard resolution raster images. Media queries and other custom solutions can assist by specifying higher resolution images for high pixel density screens. Unfortunately, increasing image resolution results in significantly more data being transferred to the device. Web traffic on mobile devices such as smartphones and tablets is on a steady growth trajectory and many mobile devices around the world use low-bandwidth connections. Communicating science effectively includes efficient delivery of the information to the reader. To meet this criteria, responsive designs should also incorporate "mobile first" elements such as serving ideal image sizes (a low resolution cell phone does not need to receive a large desktop image) and a focus on fast, readable content delivery. The technical implementation of responsive web design is constantly changing as new web standards and approaches become available. However, fundamental design principles such as grid layouts, clear typography, and proper use of white space should be an important part of content delivery within any responsive design. This presentation will discuss current responsive design approaches for improving scientific communication across multiple devices, operating systems, and bandwidth capacities. The presentation will also include example responsive designs for scientific papers and websites. Implementing a responsive design approach with a focus on content and fundamental design principles is an important step to ensuring scientific information remains clear and accessible as screens and devices continue to evolve.

Detailed Hydraulic Assessment Using a High-Resolution Piezocone Coupled to the GeoVIS

DTIC Science & Technology

2008-07-01

statistical means can be performed. • Repeat K comparisons at a highly permeable site. The piezocone is capable of estimating K in soils of higher...Version 1, March 2006, 131 pp. Ferritto, J.M., 1997. Seismic Design Criteria for Soil Liquefaction , NFESC Technical Report TR-2077-SHR, June, 1997... Soil Type and Well Design Logs................................................................... 20 Figure 6. Interpolated Three-Dimensional Head

Development and Evaluation of a Dynamic, 3-Degree-of-Freedom (DOF) Wind Tunnel Model

DTIC Science & Technology

2016-11-01

DATE (DD-MM-YYYY) November 2016 2. REPORT TYPE Contractor Report 3. DATES COVERED (From - To) November 2014–October 2015 4. TITLE AND SUBTITLE...eliminates any gimbal-induced flow disturbances. All 3 axes can be locked independently and are instrumented with high -resolution feedback. Experiments... high -speed video frames of the subsonic model in the transonic wind tunnel, Run 63

Multidecadal Rates of Disturbance- and Climate Change-Induced Land Cover Change in Arctic and Boreal Ecosystems over Western Canada and Alaska Inferred from Dense Landsat Time Series

NASA Astrophysics Data System (ADS)

Wang, J.; Sulla-menashe, D. J.; Woodcock, C. E.; Sonnentag, O.; Friedl, M. A.

2017-12-01

Rapid climate change in arctic and boreal ecosystems is driving changes to land cover composition, including woody expansion in the arctic tundra, successional shifts following boreal fires, and thaw-induced wetland expansion and forest collapse along the southern limit of permafrost. The impacts of these land cover transformations on the physical climate and the carbon cycle are increasingly well-documented from field and model studies, but there have been few attempts to empirically estimate rates of land cover change at decadal time scale and continental spatial scale. Previous studies have used too coarse spatial resolution or have been too limited in temporal range to enable broad multi-decadal assessment of land cover change. As part of NASA's Arctic Boreal Vulnerability Experiment (ABoVE), we are using dense time series of Landsat remote sensing data to map disturbances and classify land cover types across the ABoVE extended domain (spanning western Canada and Alaska) over the last three decades (1982-2014) at 30 m resolution. We utilize regionally-complete and repeated acquisition high-resolution (<2 m) DigitalGlobe imagery to generate training data from across the region that follows a nested, hierarchical classification scheme encompassing plant functional type and cover density, understory type, wetland status, and land use. Additionally, we crosswalk plot-level field data into our scheme for additional high quality training sites. We use the Continuous Change Detection and Classification algorithm to estimate land cover change dates and temporal-spectral features in the Landsat data. These features are used to train random forest classification models and map land cover and analyze land cover change processes, focusing primarily on tundra "shrubification", post-fire succession, and boreal wetland expansion. We will analyze the high resolution data based on stratified random sampling of our change maps to validate and assess the accuracy of our model predictions. In this paper, we present initial results from this effort, including sub-regional analyses focused on several key areas, such as the Taiga Plains and the Southern Arctic ecozones, to calibrate our random forest models and assess results.

Toward 10-km mesh global climate simulations

NASA Astrophysics Data System (ADS)

Ohfuchi, W.; Enomoto, T.; Takaya, K.; Yoshioka, M. K.

2002-12-01

An atmospheric general circulation model (AGCM) that runs very efficiently on the Earth Simulator (ES) was developed. The ES is a gigantic vector-parallel computer with the peak performance of 40 Tflops. The AGCM, named AFES (AGCM for ES), was based on the version 5.4.02 of an AGCM developed jointly by the Center for Climate System Research, the University of Tokyo and the Japanese National Institute for Environmental Sciences. The AFES was, however, totally rewritten in FORTRAN90 and MPI while the original AGCM was written in FORTRAN77 and not capable of parallel computing. The AFES achieved 26 Tflops (about 65 % of the peak performance of the ES) at resolution of T1279L96 (10-km horizontal resolution and 500-m vertical resolution in middle troposphere to lower stratosphere). Some results of 10- to 20-day global simulations will be presented. At this moment, only short-term simulations are possible due to data storage limitation. As ten tera flops computing is achieved, peta byte data storage are necessary to conduct climate-type simulations at this super-high resolution global simulations. Some possibilities for future research topics in global super-high resolution climate simulations will be discussed. Some target topics are mesoscale structures and self-organization of the Baiu-Meiyu front over Japan, cyclogenecsis over the North Pacific and typhoons around the Japan area. Also improvement in local precipitation with increasing horizontal resolution will be demonstrated.

A DNA methylation map of human cancer at single base-pair resolution

PubMed Central

Vidal, E; Sayols, S; Moran, S; Guillaumet-Adkins, A; Schroeder, M P; Royo, R; Orozco, M; Gut, M; Gut, I; Lopez-Bigas, N; Heyn, H; Esteller, M

2017-01-01

Although single base-pair resolution DNA methylation landscapes for embryonic and different somatic cell types provided important insights into epigenetic dynamics and cell-type specificity, such comprehensive profiling is incomplete across human cancer types. This prompted us to perform genome-wide DNA methylation profiling of 22 samples derived from normal tissues and associated neoplasms, including primary tumors and cancer cell lines. Unlike their invariant normal counterparts, cancer samples exhibited highly variable CpG methylation levels in a large proportion of the genome, involving progressive changes during tumor evolution. The whole-genome sequencing results from selected samples were replicated in a large cohort of 1112 primary tumors of various cancer types using genome-scale DNA methylation analysis. Specifically, we determined DNA hypermethylation of promoters and enhancers regulating tumor-suppressor genes, with potential cancer-driving effects. DNA hypermethylation events showed evidence of positive selection, mutual exclusivity and tissue specificity, suggesting their active participation in neoplastic transformation. Our data highlight the extensive changes in DNA methylation that occur in cancer onset, progression and dissemination. PMID:28581523

Interlaced zone plate optics for hard X-ray imaging in the 10 nm range

DOE PAGES

Mohacsi, Istvan; Vartiainen, Ismo; Rosner, Benedikt; ...

2017-03-08

Multi-keV X-ray microscopy has been particularly successful in bridging the resolution gap between optical and electron microscopy. However, resolutions below 20 nm are still considered challenging, as high throughput direct imaging methods are limited by the availability of suitable optical elements. In order to bridge this gap, we present a new type of Fresnel zone plate lenses aimed at the sub-20 and the sub-10 nm resolution range. By extending the concept of double-sided zone plate stacking, we demonstrate the doubling of the effective line density and thus the resolution and provide large aperture, single- chip optical devices with 15 andmore » 7 nm smallest zone widths. The detailed characterization of these lenses shows excellent optical properties with focal spots down to 7.8 nm. Furthermore, beyond wave front characterization, the zone plates also excel in typical imaging scenarios, verifying their resolution close to their diffraction limited optical performance.« less

Interlaced zone plate optics for hard X-ray imaging in the 10 nm range

DOE Office of Scientific and Technical Information (OSTI.GOV)

Mohacsi, Istvan; Vartiainen, Ismo; Rosner, Benedikt

Multi-keV X-ray microscopy has been particularly successful in bridging the resolution gap between optical and electron microscopy. However, resolutions below 20 nm are still considered challenging, as high throughput direct imaging methods are limited by the availability of suitable optical elements. In order to bridge this gap, we present a new type of Fresnel zone plate lenses aimed at the sub-20 and the sub-10 nm resolution range. By extending the concept of double-sided zone plate stacking, we demonstrate the doubling of the effective line density and thus the resolution and provide large aperture, single- chip optical devices with 15 andmore » 7 nm smallest zone widths. The detailed characterization of these lenses shows excellent optical properties with focal spots down to 7.8 nm. Furthermore, beyond wave front characterization, the zone plates also excel in typical imaging scenarios, verifying their resolution close to their diffraction limited optical performance.« less

Interlaced zone plate optics for hard X-ray imaging in the 10 nm range

PubMed Central

Mohacsi, Istvan; Vartiainen, Ismo; Rösner, Benedikt; Guizar-Sicairos, Manuel; Guzenko, Vitaliy A.; McNulty, Ian; Winarski, Robert; Holt, Martin V.; David, Christian

2017-01-01

Multi-keV X-ray microscopy has been particularly successful in bridging the resolution gap between optical and electron microscopy. However, resolutions below 20 nm are still considered challenging, as high throughput direct imaging methods are limited by the availability of suitable optical elements. In order to bridge this gap, we present a new type of Fresnel zone plate lenses aimed at the sub-20 and the sub-10 nm resolution range. By extending the concept of double-sided zone plate stacking, we demonstrate the doubling of the effective line density and thus the resolution and provide large aperture, singlechip optical devices with 15 and 7 nm smallest zone widths. The detailed characterization of these lenses shows excellent optical properties with focal spots down to 7.8 nm. Beyond wave front characterization, the zone plates also excel in typical imaging scenarios, verifying their resolution close to their diffraction limited optical performance.

Interlaced zone plate optics for hard X-ray imaging in the 10 nm range

NASA Astrophysics Data System (ADS)

Mohacsi, Istvan; Vartiainen, Ismo; Rösner, Benedikt; Guizar-Sicairos, Manuel; Guzenko, Vitaliy A.; McNulty, Ian; Winarski, Robert; Holt, Martin V.; David, Christian

2017-03-01

Multi-keV X-ray microscopy has been particularly successful in bridging the resolution gap between optical and electron microscopy. However, resolutions below 20 nm are still considered challenging, as high throughput direct imaging methods are limited by the availability of suitable optical elements. In order to bridge this gap, we present a new type of Fresnel zone plate lenses aimed at the sub-20 and the sub-10 nm resolution range. By extending the concept of double-sided zone plate stacking, we demonstrate the doubling of the effective line density and thus the resolution and provide large aperture, singlechip optical devices with 15 and 7 nm smallest zone widths. The detailed characterization of these lenses shows excellent optical properties with focal spots down to 7.8 nm. Beyond wave front characterization, the zone plates also excel in typical imaging scenarios, verifying their resolution close to their diffraction limited optical performance.

A digital-type fluxgate magnetometer using a sigma-delta digital-to-analog converter for a sounding rocket experiment

NASA Astrophysics Data System (ADS)

Iguchi, Kyosuke; Matsuoka, Ayako

2014-07-01

One of the design challenges for future magnetospheric satellite missions is optimizing the mass, size, and power consumption of the instruments to meet the mission requirements. We have developed a digital-type fluxgate (DFG) magnetometer that is anticipated to have significantly less mass and volume than the conventional analog-type. Hitherto, the lack of a space-grade digital-to-analog converter (DAC) with good accuracy has prevented the development of a high-performance DFG. To solve this problem, we developed a high-resolution DAC using parts whose performance was equivalent to existing space-grade parts. The developed DAC consists of a 1-bit second-order sigma-delta modulator and a fourth-order analog low-pass filter. We tested the performance of the DAC experimentally and found that it had better than 17-bits resolution in 80% of the measurement range, and the linearity error was 2-13.3 of the measurement range. We built a DFG flight model (in which this DAC was embedded) for a sounding rocket experiment as an interim step in the development of a future satellite mission. The noise of this DFG was 0.79 nTrms at 0.1-10 Hz, which corresponds to a roughly 17-bit resolution. The results show that the sigma-delta DAC and the DFG had a performance that is consistent with our optimized design, and the noise was as expected from the noise simulation. Finally, we have confirmed that the DFG worked successfully during the flight of the sounding rocket.

Stacked color image sensor using wavelength-selective organic photoconductive films with zinc-oxide thin film transistors as a signal readout circuit

NASA Astrophysics Data System (ADS)

Seo, Hokuto; Aihara, Satoshi; Namba, Masakazu; Watabe, Toshihisa; Ohtake, Hiroshi; Kubota, Misao; Egami, Norifumi; Hiramatsu, Takahiro; Matsuda, Tokiyoshi; Furuta, Mamoru; Nitta, Hiroshi; Hirao, Takashi

2010-01-01

Our group has been developing a new type of image sensor overlaid with three organic photoconductive films, which are individually sensitive to only one of the primary color components (blue (B), green (G), or red (R) light), with the aim of developing a compact, high resolution color camera without any color separation optical systems. In this paper, we firstly revealed the unique characteristics of organic photoconductive films. Only choosing organic materials can tune the photoconductive properties of the film, especially excellent wavelength selectivities which are good enough to divide the incident light into three primary colors. Color separation with vertically stacked organic films was also shown. In addition, the high-resolution of organic photoconductive films sufficient for high-definition television (HDTV) was confirmed in a shooting experiment using a camera tube. Secondly, as a step toward our goal, we fabricated a stacked organic image sensor with G- and R-sensitive organic photoconductive films, each of which had a zinc oxide (ZnO) thin film transistor (TFT) readout circuit, and demonstrated image pickup at a TV frame rate. A color image with a resolution corresponding to the pixel number of the ZnO TFT readout circuit was obtained from the stacked image sensor. These results show the potential for the development of high-resolution prism-less color cameras with stacked organic photoconductive films.

A climatology of visible surface reflectance spectra

NASA Astrophysics Data System (ADS)

Zoogman, Peter; Liu, Xiong; Chance, Kelly; Sun, Qingsong; Schaaf, Crystal; Mahr, Tobias; Wagner, Thomas

2016-09-01

We present a high spectral resolution climatology of visible surface reflectance as a function of wavelength for use in satellite measurements of ozone and other atmospheric species. The Tropospheric Emissions: Monitoring of Pollution (TEMPO) instrument is planned to measure backscattered solar radiation in the 290-740 nm range, including the ultraviolet and visible Chappuis ozone bands. Observation in the weak Chappuis band takes advantage of the relative transparency of the atmosphere in the visible to achieve sensitivity to near-surface ozone. However, due to the weakness of the ozone absorption features this measurement is more sensitive to errors in visible surface reflectance, which is highly variable. We utilize reflectance measurements of individual plant, man-made, and other surface types to calculate the primary modes of variability of visible surface reflectance at a high spectral resolution, comparable to that of TEMPO (0.6 nm). Using the Moderate-resolution Imaging Spectroradiometer (MODIS) Bidirection Reflectance Distribution Function (BRDF)/albedo product and our derived primary modes we construct a high spatial resolution climatology of wavelength-dependent surface reflectance over all viewing scenes and geometries. The Global Ozone Monitoring Experiment-2 (GOME-2) Lambertian Equivalent Reflectance (LER) product provides complementary information over water and snow scenes. Preliminary results using this approach in multispectral ultraviolet+visible ozone retrievals from the GOME-2 instrument show significant improvement to the fitting residuals over vegetated scenes.

Mapping Spatial Variability in Health and Wealth Indicators in Accra, Ghana Using High Spatial Resolution Imagery

NASA Astrophysics Data System (ADS)

Engstrom, R.; Ashcroft, E.

2014-12-01

There has been a tremendous amount of research conducted that examines disparities in health and wealth of persons between urban and rural areas however, relatively little research has been undertaken to examine variations within urban areas. A major limitation to elucidating differences with urban areas is the lack of social and demographic data at a sufficiently high spatial resolution to determine these differences. Generally the only available data that contain this information are census data which are collected at most every ten years and are often difficult to obtain at a high enough spatial resolution to allow for examining in depth variability in health and wealth indicators at high spatial resolutions, especially in developing countries. High spatial resolution satellite imagery may be able to provide timely and synoptic information that is related to health and wealth variability within a city. In this study we use two dates of Quickbird imagery (2003 and 2010) classified into the vegetation-impervious surface-soil (VIS) model introduced by Ridd (1995). For 2003 we only have partial coverage of the city, while for 2010 we have a mosaic, which covers the entire city of Accra, Ghana. Variations in the VIS values represent the physical variations within the city and these are compared to variations in economic, and/or sociodemographic data derived from the 2000 Ghanaian census at two spatial resolutions, the enumeration area (approximately US Census Tract) and the neighborhood for the city. Results indicate a significant correlation between both vegetation and impervious surface to type of cooking fuel used in the household, population density, housing density, availability of sewers, cooking space usage, and other variables. The correlations are generally stronger at the neighborhood level and the relationships are stable through time and space. Overall, the results indicate that information derived from high resolution satellite data is related to indicators of health and wealth within a developing world city and that the even if the imagery is collected 10 years after the census information, the relationships are still significant.

Mass loss in red giants and supergiants

NASA Technical Reports Server (NTRS)

Sanner, F.

1976-01-01

The circumstellar envelopes surrounding 13 late-type giants and supergiants have been studied using a homogeneous collection of high-resolution, photoelectric scans of strong optical resonance lines. Various properties of the envelopes, including the mass loss rate, dilution factor, hydrogen density, and degree of ionization, have been determined quantitatively.

A high-resolution map of the three-dimensional chromatin interactome in human cells.

PubMed

Jin, Fulai; Li, Yan; Dixon, Jesse R; Selvaraj, Siddarth; Ye, Zhen; Lee, Ah Young; Yen, Chia-An; Schmitt, Anthony D; Espinoza, Celso A; Ren, Bing

2013-11-14

A large number of cis-regulatory sequences have been annotated in the human genome, but defining their target genes remains a challenge. One strategy is to identify the long-range looping interactions at these elements with the use of chromosome conformation capture (3C)-based techniques. However, previous studies lack either the resolution or coverage to permit a whole-genome, unbiased view of chromatin interactions. Here we report a comprehensive chromatin interaction map generated in human fibroblasts using a genome-wide 3C analysis method (Hi-C). We determined over one million long-range chromatin interactions at 5-10-kb resolution, and uncovered general principles of chromatin organization at different types of genomic features. We also characterized the dynamics of promoter-enhancer contacts after TNF-α signalling in these cells. Unexpectedly, we found that TNF-α-responsive enhancers are already in contact with their target promoters before signalling. Such pre-existing chromatin looping, which also exists in other cell types with different extracellular signalling, is a strong predictor of gene induction. Our observations suggest that the three-dimensional chromatin landscape, once established in a particular cell type, is relatively stable and could influence the selection or activation of target genes by a ubiquitous transcription activator in a cell-specific manner.

High-resolution confocal imaging of wall ingrowth deposition in plant transfer cells: Semi-quantitative analysis of phloem parenchyma transfer cell development in leaf minor veins of Arabidopsis.

PubMed

Nguyen, Suong T T; McCurdy, David W

2015-04-23

Transfer cells (TCs) are trans-differentiated versions of existing cell types designed to facilitate enhanced membrane transport of nutrients at symplasmic/apoplasmic interfaces. This transport capacity is conferred by intricate wall ingrowths deposited secondarily on the inner face of the primary cell wall, hence promoting the potential trans-membrane flux of solutes and consequently assigning TCs as having key roles in plant growth and productivity. However, TCs are typically positioned deep within tissues and have been studied mostly by electron microscopy. Recent advances in fluorophore labelling of plant cell walls using a modified pseudo-Schiff-propidium iodide (mPS-PI) staining procedure in combination with high-resolution confocal microscopy have allowed visualization of cellular details of individual tissue layers in whole mounts, hence enabling study of tissue and cellular architecture without the need for tissue sectioning. Here we apply a simplified version of the mPS-PI procedure for confocal imaging of cellulose-enriched wall ingrowths in vascular TCs at the whole tissue level. The simplified mPS-PI staining procedure produced high-resolution three-dimensional images of individual cell types in vascular bundles and, importantly, wall ingrowths in phloem parenchyma (PP) TCs in minor veins of Arabidopsis leaves and companion cell TCs in pea. More efficient staining of tissues was obtained by replacing complex clearing procedures with a simple post-fixation bleaching step. We used this modified procedure to survey the presence of PP TCs in other tissues of Arabidopsis including cotyledons, cauline leaves and sepals. This high-resolution imaging enabled us to classify different stages of wall ingrowth development in Arabidopsis leaves, hence enabling semi-quantitative assessment of the extent of wall ingrowth deposition in PP TCs at the whole leaf level. Finally, we conducted a defoliation experiment as an example of using this approach to statistically analyze responses of PP TC development to leaf ablation. Use of a modified mPS-PI staining technique resulted in high-resolution confocal imaging of polarized wall ingrowth deposition in TCs. This technique can be used in place of conventional electron microscopy and opens new possibilities to study mechanisms determining polarized deposition of wall ingrowths and use reverse genetics to identify regulatory genes controlling TC trans-differentiation.

Synergistic use of MODIS cloud products and AIRS radiance measurements for retrieval of cloud parameters

NASA Astrophysics Data System (ADS)

Li, J.; Menzel, W.; Sun, F.; Schmit, T.

2003-12-01

The Moderate-Resolution Imaging Spectroradiometer (MODIS) and Atmospheric Infrared Sounder (AIRS) measurements from the Earth Observing System's (EOS) Aqua satellite will enable global monitoring of the distribution of clouds. MODIS is able to provide at high spatial resolution (1 ~ 5km) the cloud mask, surface and cloud types, cloud phase, cloud-top pressure (CTP), effective cloud amount (ECA), cloud particle size (CPS), and cloud water path (CWP). AIRS is able to provide CTP, ECA, CPS, and CWP within the AIRS footprint with much better accuracy using its greatly enhanced hyperspectral remote sensing capability. The combined MODIS / AIRS system offers the opportunity for cloud products improved over those possible from either system alone. The algorithm developed was applied to process the AIRS longwave cloudy radiance measurements; results are compared with MODIS cloud products, as well as with the Geostationary Operational Environmental Satellite (GOES) sounder cloud products, to demonstrate the advantage of synergistic use of high spatial resolution MODIS cloud products and high spectral resolution AIRS sounder radiance measurements for optimal cloud retrieval. Data from ground-based instrumentation at the Atmospheric Radiation Measurement (ARM) Program Cloud and Radiation Test Bed (CART) in Oklahoma were used for the validation; results show that AIRS improves the MODIS cloud products in certain cases such as low-level clouds.

The High-energy Burst Spectrometer for SMESE Mission

NASA Astrophysics Data System (ADS)

Gu, Qiang; Chang, Jin

2009-01-01

The SMall Explorer for Solar Eruptions (SMESE) is a small satellite being developed jointly by China and France. It is planed to launch around the next solar maximum year (˜ 2011) for observing simultaneously the two most violent types of eruptive events on the sun (the coronal mass ejection (CME) and the solar flare) and investigating their relationship. As one of the 3 main payloads of the small satellite, the high energy burst spectrometer (HEBS) adopts the upto- date high-resolution LaBr3 scintillation detector to observe the high-energy solar radiation in the range 10 keV—600 MeV. Its energy resolution is better than 3.0% at 662 keV, 2-fold higher than that of current scintillation detectors, promising a breakthrough in the studies of energy release in solar flares and CMEs, particle acceleration and the relationship between solar flares and CMEs.

A Survey of Near-infrared Diffuse Interstellar Bands

NASA Astrophysics Data System (ADS)

Hamano, Satochi; Kobayashi, Naoto; Kawakita, Hideyo; Ikeda, Yuji; Kondo, Sohei; Sameshima, Hiroaki; Arai, Akira; Matsunaga, Noriyuki; Yasui, Chikako; Mizumoto, Misaki; Fukue, Kei; Izumi, Natsuko; Otsubo, Shogo; Takenada, Keiichi

2018-04-01

We propose a study of interstellar molecules with near-infrared (NIR) high-resolution spectroscopy as a science case for the 3.6-m Devasthal Optical Telescope (DOT). In particular, we present the results obtained on-going survey of diffuse interstellar bands (DIBs) in NIR with the newly developed NIR high-resolution spectrograph WINERED, which offers a high sensitivity in the wavelength range of 0.91-1.36 µm. Using the WINERED spectrograph attached to the 1.3-m Araki telescope in Japan, we obtained high-quality spectra of a number of early-type stars in various environments, such as diffuse interstellar clouds, dark clouds and star-forming regions, to investigate the properties of NIR DIBs and constrain their carriers. As a result, we successfully identified about 50 new NIR DIBs, where only five fairly strong DIBs had been identified previously. Also, some properties of DIBs in the NIR are discussed to constrain the carriers of DIBs.

Vii. New Kr IV - VII Oscillator Strengths and an Improved Spectral Analysis of the Hot, Hydrogen-deficient Do-type White Dwarf RE 0503-289

NASA Technical Reports Server (NTRS)

Rauch, T.; Quinet, P.; Hoyer, D.; Werner, K.; Richter, P.; Kruk, J. W.; Demleitner, M.

2016-01-01

For the spectral analysis of high-resolution and high signal-to-noise (SN) spectra of hot stars, state-of-the-art non-local thermodynamic equilibrium (NLTE) model atmospheres are mandatory. These are strongly dependent on the reliability of the atomic data that is used for their calculation. Aims. New Krivvii oscillator strengths for a large number of lines enable us to construct more detailed model atoms for our NLTEmodel-atmosphere calculations. This enables us to search for additional Kr lines in observed spectra and to improve Kr abundance determinations. Methods. We calculated Krivvii oscillator strengths to consider radiative and collisional bound-bound transitions in detail in our NLTE stellar-atmosphere models for the analysis of Kr lines that are exhibited in high-resolution and high SN ultraviolet (UV)observations of the hot white dwarf RE 0503.

Multiplicity of the Galactic Senior Citizens: A high-resolution search for cool subdwarf companions

NASA Astrophysics Data System (ADS)

Ziegler, Carl; Law, Nicholas M.

2015-01-01

Cool subdwarfs, with spectral types late K and M, are the oldest members of the low-mass stellar population. Mostly present in the galactic halo, subdwarfs are characterized by their low metallicity and high proper-motions. Understanding their binary fraction could give key insights into the star formation process early in the Milky Way's history. However, because of their low luminosity and relative rarity in the solar neighborhood, binary surveys of cool subdwarfs have suffered from small sample sizes and large incompleteness gaps. It appears, however, that the binary fraction of red subdwarfs is much lower than for their main-sequence cousins. Using the highly efficient Robo-AO system, we present the largest high-resolution survey of subdwarfs yet. We find from 349 target cool subdwarfs, 39 are in multiple systems, 13 newly discovered, for a binary fraction of 11 ± 1.8%.

A high-stability scanning tunneling microscope achieved by an isolated tiny scanner with low voltage imaging capability.

PubMed

Wang, Qi; Hou, Yubin; Wang, Junting; Lu, Qingyou

2013-11-01

We present a novel homebuilt scanning tunneling microscope (STM) with high quality atomic resolution. It is equipped with a small but powerful GeckoDrive piezoelectric motor which drives a miniature and detachable scanning part to implement coarse approach. The scanning part is a tiny piezoelectric tube scanner (industry type: PZT-8, whose d31 coefficient is one of the lowest) housed in a slightly bigger polished sapphire tube, which is riding on and spring clamped against the knife edges of a tungsten slot. The STM so constructed shows low back-lashing and drifting and high repeatability and immunity to external vibrations. These are confirmed by its low imaging voltages, low distortions in the spiral scanned images, and high atomic resolution quality even when the STM is placed on the ground of the fifth floor without any external or internal vibration isolation devices.

Flexible sample environment for high resolution neutron imaging at high temperatures in controlled atmosphere

DOE Office of Scientific and Technical Information (OSTI.GOV)

Makowska, Małgorzata G.; Theil Kuhn, Luise; Cleemann, Lars N.

In high material penetration by neutrons allows for experiments using sophisticated sample environments providing complex conditions. Thus, neutron imaging holds potential for performing in situ nondestructive measurements on large samples or even full technological systems, which are not possible with any other technique. Our paper presents a new sample environment for in situ high resolution neutron imaging experiments at temperatures from room temperature up to 1100 degrees C and/or using controllable flow of reactive atmospheres. The design also offers the possibility to directly combine imaging with diffraction measurements. Design, special features, and specification of the furnace are described. In addition,more » examples of experiments successfully performed at various neutron facilities with the furnace, as well as examples of possible applications are presented. Our work covers a broad field of research from fundamental to technological investigations of various types of materials and components.« less

Flexible sample environment for high resolution neutron imaging at high temperatures in controlled atmosphere

DOE PAGES

Makowska, Małgorzata G.; Theil Kuhn, Luise; Cleemann, Lars N.; ...

2015-12-17

In high material penetration by neutrons allows for experiments using sophisticated sample environments providing complex conditions. Thus, neutron imaging holds potential for performing in situ nondestructive measurements on large samples or even full technological systems, which are not possible with any other technique. Our paper presents a new sample environment for in situ high resolution neutron imaging experiments at temperatures from room temperature up to 1100 degrees C and/or using controllable flow of reactive atmospheres. The design also offers the possibility to directly combine imaging with diffraction measurements. Design, special features, and specification of the furnace are described. In addition,more » examples of experiments successfully performed at various neutron facilities with the furnace, as well as examples of possible applications are presented. Our work covers a broad field of research from fundamental to technological investigations of various types of materials and components.« less

Accurate Typing of Human Leukocyte Antigen Class I Genes by Oxford Nanopore Sequencing.

PubMed

Liu, Chang; Xiao, Fangzhou; Hoisington-Lopez, Jessica; Lang, Kathrin; Quenzel, Philipp; Duffy, Brian; Mitra, Robi David

2018-04-03

Oxford Nanopore Technologies' MinION has expanded the current DNA sequencing toolkit by delivering long read lengths and extreme portability. The MinION has the potential to enable expedited point-of-care human leukocyte antigen (HLA) typing, an assay routinely used to assess the immunologic compatibility between organ donors and recipients, but the platform's high error rate makes it challenging to type alleles with accuracy. We developed and validated accurate typing of HLA by Oxford nanopore (Athlon), a bioinformatic pipeline that i) maps nanopore reads to a database of known HLA alleles, ii) identifies candidate alleles with the highest read coverage at different resolution levels that are represented as branching nodes and leaves of a tree structure, iii) generates consensus sequences by remapping the reads to the candidate alleles, and iv) calls the final diploid genotype by blasting consensus sequences against the reference database. Using two independent data sets generated on the R9.4 flow cell chemistry, Athlon achieved a 100% accuracy in class I HLA typing at the two-field resolution. Copyright © 2018 American Society for Investigative Pathology and the Association for Molecular Pathology. Published by Elsevier Inc. All rights reserved.

Metabolic profiling of Arabidopsis thaliana epidermal cells

PubMed Central

Ebert, Berit; Zöller, Daniela; Erban, Alexander; Fehrle, Ines; Hartmann, Jürgen; Niehl, Annette; Kopka, Joachim; Fisahn, Joachim

2010-01-01

Metabolic phenotyping at cellular resolution may be considered one of the challenges in current plant physiology. A method is described which enables the cell type-specific metabolic analysis of epidermal cell types in Arabidopsis thaliana pavement, basal, and trichome cells. To achieve the required high spatial resolution, single cell sampling using microcapillaries was combined with routine gas chromatography-time of flight-mass spectrometry (GC-TOF-MS) based metabolite profiling. The identification and relative quantification of 117 mostly primary metabolites has been demonstrated. The majority, namely 90 compounds, were accessible without analytical background correction. Analyses were performed using cell type-specific pools of 200 microsampled individual cells. Moreover, among these identified metabolites, 38 exhibited differential pool sizes in trichomes, basal or pavement cells. The application of an independent component analysis confirmed the cell type-specific metabolic phenotypes. Significant pool size changes between individual cells were detectable within several classes of metabolites, namely amino acids, fatty acids and alcohols, alkanes, lipids, N-compounds, organic acids and polyhydroxy acids, polyols, sugars, sugar conjugates and phenylpropanoids. It is demonstrated here that the combination of microsampling and GC-MS based metabolite profiling provides a method to investigate the cellular metabolism of fully differentiated plant cell types in vivo. PMID:20150518

Microchip assays for screening monoclonal antibody product quality.

PubMed

Chen, Xiaoyu; Tang, Kaiyan; Lee, Maximilian; Flynn, Gregory C

2008-12-01

Microchip CE-SDS was evaluated as a high-throughput alternative to conventional CE-SDS for monitoring monoclonal antibody protein quality. A commercial instrument (LabChip) 90) was used to separate dodecyl sulfate coated proteins through a sieving polymer based on the proteins' sizes. Under reducing conditions, the microchip CE-SDS separation was similar to that of conventional CE-SDS, providing reasonable resolution of the non-glycosylated and the glycosylated heavy chains. The fluorescence detection on LabChip 90 using non-covalent fluorescent labeling method was about as sensitive as the 220 nm UV detection used in a conventional CE instrument. A simple glycan typing assay was developed for the reducing microchip CE-SDS format. Antibodies, either pure or in crude cell culture media are treated with Endoglycosidase H, which specifically cleaves the hybrid and high mannose type glycans. A heavy chain migration shift on reducing CE-SDS resulting from the loss of glycan is used to measure the level of high mannose/hybrid type glycans as a percentage of the total glycans. Microchip CE-SDS, under both non-reducing and reducing conditions, can be used in a variety of antibody product screening assays. The microchip analyses provide sufficient resolution and sensitivity for this purpose but on a time scale approximately 70 times faster (41 s versus 50 min per sample) than conventional CE separation under typical operational conditions.

Towards High-Resolution Tissue Imaging Using Nanospray Desorption Electrospray Ionization Mass Spectrometry Coupled to Shear Force Microscopy

NASA Astrophysics Data System (ADS)

Nguyen, Son N.; Sontag, Ryan L.; Carson, James P.; Corley, Richard A.; Ansong, Charles; Laskin, Julia

2018-02-01

Constant mode ambient mass spectrometry imaging (MSI) of tissue sections with high lateral resolution of better than 10 μm was performed by combining shear force microscopy with nanospray desorption electrospray ionization (nano-DESI). Shear force microscopy enabled precise control of the distance between the sample and nano-DESI probe during MSI experiments and provided information on sample topography. Proof-of-concept experiments were performed using lung and brain tissue sections representing spongy and dense tissues, respectively. Topography images obtained using shear force microscopy were comparable to the results obtained using contact profilometry over the same region of the tissue section. Variations in tissue height were found to be dependent on the tissue type and were in the range of 0-5 μm for lung tissue and 0-3 μm for brain tissue sections. Ion images of phospholipids obtained in this study are in good agreement with literature data. Normalization of nano-DESI MSI images to the signal of the internal standard added to the extraction solvent allowed us to construct high-resolution ion images free of matrix effects.

Observations of Exoplanets with the Stratospheric Observatory for Infrared Astronomy (SOFIA)

NASA Astrophysics Data System (ADS)

Gehrz, R.; Becklin, E.

2010-10-01

The joint U.S. and German Stratospheric Observatory for Infrared Astronomy (SOFIA) is a 2.5-meter infrared airborne telescope in a Boeing 747-SP that will begin science flights in 2010. Flying in the stratosphere at altitudes as high as 45,000 feet, SOFIA will be used to conduct photometric, spectroscopic, and imaging observations at wavelengths from 0.3 microns to 1.9 millimeters with an average transmission of greater than 80 percent. SOFIA's first-generation instrument complement includes high speed photometers, broadband imagers, moderate resolution spectrographs capable of resolving broad features due to dust and large molecules, and high resolution spectrometers suitable for kinematic studies of molecular and atomic gas lines at km/s resolution. These and future instruments will give SOFIA the potential to make unique contributions to the characterization of the atmospheres of exoplanets that transit their parent stars. First-light images obtained on May 26, 2010 with the FORCAST imager will be shown. We will discuss several types of experiments that are being contemplated with respect to observations of exoplanets .

High-Resolution Study of the Perturbation in the CO Triplet Band

NASA Astrophysics Data System (ADS)

Momona, M.; Kanamori, H.; Sakurai, K.

1993-05-01

Seven hundred absorption lines have been observed in the discharge plasma of He and CO in the 12600-12 800 cm-1 region with Doppler-limited resolution by a near-infrared diode laser spectrometer. Out of complex spectral patterns, more than 400 lines were assigned to the CO triplet band, d3Δ(v‧ = 2) - a3Π (v″ = 1). The upper state of this transition is known to be severely perturbed. The measurement of all the spin subbands of the 3Δ - 3Π transition allowed us to reanalyze the perturbation of the d3Δ (v = 2) state with the highly vibrationally excited state, v = 9, in the a3Π state. Diode laser spectroscopy with high sensitivity and Doppler-limited resolution revealed the overtone band transition from v = 1 to v = 9 within the a3Π state and the Λ-type doubling in the d3Δ state. Those interesting phenomena can be understood as a result of the perturbation and were successfully reproduced by the eigenvectors determined in this analysis.

Scattering-type scanning near-field optical microscopy with reconstruction of vertical interaction

PubMed Central

Wang, Le; Xu, Xiaoji G.

2015-01-01

Scattering-type scanning near-field optical microscopy provides access to super-resolution spectroscopic imaging of the surfaces of a variety of materials and nanostructures. In addition to chemical identification, it enables observations of nano-optical phenomena, such as mid-infrared plasmons in graphene and phonon polaritons in boron nitride. Despite the high lateral spatial resolution, scattering-type near-field optical microscopy is not able to provide characteristics of near-field responses in the vertical dimension, normal to the sample surface. Here, we present an accurate and fast reconstruction method to obtain vertical characteristics of near-field interactions. For its first application, we investigated the bound electromagnetic field component of surface phonon polaritons on the surface of boron nitride nanotubes and found that it decays within 20 nm with a considerable phase change in the near-field signal. The method is expected to provide characterization of the vertical field distribution of a wide range of nano-optical materials and structures. PMID:26592949

High-resolution FTIR spectroscopy of the Coriolis interacting nu3 and nu9 fundamentals of methylene fluoride-d2

NASA Astrophysics Data System (ADS)

Goh, K. L.; Tan, T. L.; Ong, P. P.; Chaw, K. H.; Teo, H. H.

The Fourier transform infrared spectrum of the υ3 and υ9 bands of methylene fluoride-d2 (CD2F2) has been recorded with an unapodized resolution of 0.0024cm-1 in the frequency range 970-1080cm-1. These two bands with band centres approximately 26 cm-1 apart were mutually coupled by Coriolis interactions. By fitting a total of 1639 infrared transitions of both υ3 and υ9 with a standard deviation of 0.00084cm-1 S/S using a Watson's A-reduced Hamiltonian in the Ir representation with the inclusion of a first order c-type Coriolis resonance term, two sets of rovibrational constants for υ3 = 1 and υ9 = 1 states were derived. The υ3 band is B-type while the υ9 band is A-type with band centres at 1030.1573 ± 0.0003 and 1003.7435 ± 0.0001cm-1, respectively.

Contrast-to-noise ratio with different settings in a CBCT machine in presence of different root-end filling materials: an in vitro study.

PubMed

Demirturk Kocasarac, Husniye; Helvacioglu Yigit, Dilek; Bechara, Boulos; Sinanoglu, Alper; Noujeim, Marcel

2016-01-01

To compare the contrast-to-noise ratio (CNR) of multiple acquisition settings for four types of retrograde filling materials in CBCT images taken for endodontic surgery follow-up. 20 maxillary central incisors were endodontically treated and obturated with 4 different root-end filling materials: amalgam, mineral trioxide aggregate, SuperEBA(™) (Harry J Bosworth Company, Skokie, IL) and Biodentine™ (Septodont, Saint-Maur-des-Faussés, France). Teeth were placed in a skull and scanned, one by one, with the Planmeca ProMax(®) 3D Max (Planmeca, Helsinki, Finland); at different voltages: 66, 76, 84 and 96 kVp; with low, normal and high resolution and high definition (HD); with and without metal artefact reduction (MAR). Images were analyzed using ImageJ software (National Institutes of Health, Bethesda, MD) to calculate the CNR. The dose-area product was registered, and the effective dose calculated. No statistically significant difference was noted between the four materials. 84 and 96 kVp with low resolution and the use of MAR-generated images that have statistically better CNR than 66 and 76 kVp with HD, normal and high resolutions and without MAR. The use of low resolution also generated the smallest value of effective dose. The best setting for radiographic follow-up in an endodontic surgery with retrograde filling on the Planmeca ProMax is 96 kVp with low resolution and high MAR; this setting produced one of the lowest effective doses.

GLAST Burst Monitor Signal Processing System

NASA Astrophysics Data System (ADS)

Bhat, P. Narayana; Briggs, Michael; Connaughton, Valerie; Diehl, Roland; Fishman, Gerald; Greiner, Jochen; Kippen, R. Marc; von Kienlin, Andreas; Kouveliotou, Chryssa; Lichti, Giselher; Meegan, Charles; Paciesas, William; Persyn, Steven; Preece, Robert; Steinle, Helmut; Wilson-Hodge, Colleen

2007-07-01

The onboard Data Processing Unit (DPU), designed and built by Southwest Research Institute, performs the high-speed data acquisition for GBM. The analog signals from each of the 14 detectors are digitized by high-speed multichannel analog data acquisition architecture. The streaming digital values resulting from a periodic (period of 104.2 ns) sampling of the analog signal by the individual ADCs are fed to a Field-Programmable Gate Array (FPGA). Real-time Digital Signal Processing (DSP) algorithms within the FPGA implement functions like filtering, thresholding, time delay and pulse height measurement. The spectral data with a 12-bit resolution are formatted according to the commandable look-up-table (LUT) and then sent to the High-Speed Science-Date Bus (HSSDB, speed=1.5 MB/s) to be telemetered to ground. The DSP offers a novel feature of a commandable & constant event deadtime. The ADC non-linearities have been calibrated so that the spectral data can be corrected during analysis. The best temporal resolution is 2 μs for the pre-burst & post-trigger time-tagged events (TTE) data. The time resolution of the binned data types is commandable from 64 msec to 1.024 s for the CTIME data (8 channel spectral resolution) and 1.024 to 32.768 s for the CSPEC data (128 channel spectral resolution). The pulse pile-up effects have been studied by Monte Carlo simulations. For a typical GRB, the possible shift in the Epeak value at high-count rates (~100 kHz) is ~1% while the change in the single power-law index could be up to 5%.

Integrating Landsat Data and High-Resolution Imagery for Applied Conservation Assessment of Forest Cover in Latin American Heterogenous Landscapes

NASA Astrophysics Data System (ADS)

Thomas, N.; Rueda, X.; Lambin, E.; Mendenhall, C. D.

2012-12-01

Large intact forested regions of the world are known to be critical to maintaining Earth's climate, ecosystem health, and human livelihoods. Remote sensing has been successfully implemented as a tool to monitor forest cover and landscape dynamics over broad regions. Much of this work has been done using coarse resolution sensors such as AVHRR and MODIS in combination with moderate resolution sensors, particularly Landsat. Finer scale analysis of heterogeneous and fragmented landscapes is commonly performed with medium resolution data and has had varying success depending on many factors including the level of fragmentation, variability of land cover types, patch size, and image availability. Fine scale tree cover in mixed agricultural areas can have a major impact on biodiversity and ecosystem sustainability but may often be inadequately captured with the global to regional (coarse resolution and moderate resolution) satellite sensors and processing techniques widely used to detect land use and land cover changes. This study investigates whether advanced remote sensing methods are able to assess and monitor percent tree canopy cover in spatially complex human-dominated agricultural landscapes that prove challenging for traditional mapping techniques. Our study areas are in high altitude, mixed agricultural coffee-growing regions in Costa Rica and the Colombian Andes. We applied Random Forests regression tree analysis to Landsat data along with additional spectral, environmental, and spatial variables to predict percent tree canopy cover at 30m resolution. Image object-based texture, shape, and neighborhood metrics were generated at the Landsat scale using eCognition and included in the variable suite. Training and validation data was generated using high resolution imagery from digital aerial photography at 1m to 2.5 m resolution. Our results are promising with Pearson's correlation coefficients between observed and predicted percent tree canopy cover of .86 (Costa Rica) and .83 (Colombia). The tree cover mapping developed here supports two distinct projects on sustaining biodiversity and natural and human capital: in Costa Rica the tree canopy cover map is utilized to predict bird community composition; and in Colombia the mapping is performed for two time periods and used to assess the impact of coffee eco-certification programs on the landscape. This research identifies ways to leverage readily available, high quality, and cost-free Landsat data or other medium resolution satellite data sources in combination with high resolution data, such as that frequently available through Google Earth, to monitor and support sustainability efforts in fragmented and heterogeneous landscapes.

High definition clouds and precipitation for climate prediction -results from a unified German research initiative on high resolution modeling and observations

NASA Astrophysics Data System (ADS)

Rauser, F.

2013-12-01

We present results from the German BMBF initiative 'High Definition Cloud and Precipitation for advancing Climate Prediction -HD(CP)2'. This initiative addresses most of the problems that are discussed in this session in one, unified approach: cloud physics, convection, boundary layer development, radiation and subgrid variability are approached in one organizational framework. HD(CP)2 merges both observation and high performance computing / model development communities to tackle a shared problem: how to improve the understanding of the most important subgrid-scale processes of cloud and precipitation physics, and how to utilize this knowledge for improved climate predictions. HD(CP)2 is a coordinated initiative to: (i) realize; (ii) evaluate; and (iii) statistically characterize and exploit for the purpose of both parameterization development and cloud / precipitation feedback analysis; ultra-high resolution (100 m in the horizontal, 10-50 m in the vertical) regional hind-casts over time periods (3-15 y) and spatial scales (1000-1500 km) that are climatically meaningful. HD(CP)2 thus consists of three elements (the model development and simulations, their observational evaluation and exploitation/synthesis to advance CP prediction) and its first three-year phase has started on October 1st 2012. As a central part of HD(CP)2, the HD(CP)2 Observational Prototype Experiment (HOPE) has been carried out in spring 2013. In this campaign, high resolution measurements with a multitude of instruments from all major centers in Germany have been carried out in a limited domain, to allow for unprecedented resolution and precision in the observation of microphysics parameters on a resolution that will allow for evaluation and improvement of ultra-high resolution models. At the same time, a local area version of the new climate model ICON of the Max Planck Institute and the German weather service has been developed that allows for LES-type simulations on high resolutions on limited domains. The advantage of modifying an existing, evolving climate model is to share insights from high resolution runs directly with the large-scale modelers and to allow for easy intercomparison and evaluation later on. Within this presentation, we will give a short overview on HD(CP)2 , show results from the observation campaign HOPE and the LES simulations of the same domain and conditions and will discuss how these will lead to an improved understanding and evaluation background for the efforts to improve fast physics in our climate model.

Measuring Ambiguity in HLA Typing Methods

PubMed Central

Madbouly, Abeer; Freeman, John; Maiers, Martin

2012-01-01

In hematopoietic stem cell transplantation, donor selection is based primarily on matching donor and patient HLA genes. These genes are highly polymorphic and their typing can result in exact allele assignment at each gene (the resolution at which patients and donors are matched), but it can also result in a set of ambiguous assignments, depending on the typing methodology used. To facilitate rapid identification of matched donors, registries employ statistical algorithms to infer HLA alleles from ambiguous genotypes. Linkage disequilibrium information encapsulated in haplotype frequencies is used to facilitate prediction of the most likely haplotype assignment. An HLA typing with less ambiguity produces fewer high-probability haplotypes and a more reliable prediction. We estimated ambiguity for several HLA typing methods across four continental populations using an information theory-based measure, Shannon's entropy. We used allele and haplotype frequencies to calculate entropy for different sets of 1,000 subjects with simulated HLA typing. Using allele frequencies we calculated an average entropy in Caucasians of 1.65 for serology, 1.06 for allele family level, 0.49 for a 2002-era SSO kit, and 0.076 for single-pass SBT. When using haplotype frequencies in entropy calculations, we found average entropies of 0.72 for serology, 0.73 for allele family level, 0.05 for SSO, and 0.002 for single-pass SBT. Application of haplotype frequencies further reduces HLA typing ambiguity. We also estimated expected confirmatory typing mismatch rates for simulated subjects. In a hypothetical registry with all donors typed using the same method, the entropy values based on haplotype frequencies correspond to confirmatory typing mismatch rates of 1.31% for SSO versus only 0.08% for SBT. Intermediate-resolution single-pass SBT contains the least ambiguity of the methods we evaluated and therefore the most certainty in allele prediction. The presented measure objectively evaluates HLA typing methods and can help define acceptable HLA typing for donor recruitment. PMID:22952712

Modeling soil temperature change in Seward Peninsula, Alaska

NASA Astrophysics Data System (ADS)

Debolskiy, M. V.; Nicolsky, D.; Romanovsky, V. E.; Muskett, R. R.; Panda, S. K.

2017-12-01

Increasing demand for assessment of climate change-induced permafrost degradation and its consequences promotes creation of high-resolution modeling products of soil temperature changes. This is especially relevant for areas with highly vulnerable warm discontinuous permafrost in the Western Alaska. In this study, we apply ecotype-based modeling approach to simulate high-resolution permafrost distribution and its temporal dynamics in Seward Peninsula, Alaska. To model soil temperature dynamics, we use a transient soil heat transfer model developed at the Geophysical Institute Permafrost Laboratory (GIPL-2). The model solves one dimensional nonlinear heat equation with phase change. The developed model is forced with combination of historical climate and different future scenarios for 1900-2100 with 2x2 km resolution prepared by Scenarios Network for Alaska and Arctic Planning (2017). Vegetation, snow and soil properties are calibrated by ecotype and up-scaled by using Alaska Existing Vegetation Type map for Western Alaska (Flemming, 2015) with 30x30 m resolution provided by Geographic Information Network of Alaska (UAF). The calibrated ecotypes cover over 75% of the study area. We calibrate the model using a data assimilation technique utilizing available observations of air, surface and sub-surface temperatures and snow cover collected by various agencies and research groups (USGS, Geophysical Institute, USDA). The calibration approach takes into account a natural variability between stations in the same ecotype and finds an optimal set of model parameters (snow and soil properties) within the study area. This approach allows reduction in microscale heterogeneity and aggregated soil temperature data from shallow boreholes which is highly dependent on local conditions. As a result of this study we present a series of preliminary high resolution maps for the Seward Peninsula showing changes in the active layer depth and ground temperatures for the current climate and future climate change scenarios.

High-Resolution Three-Dimensional Computed Tomography for Assessing Complications Related to Intrathecal Drug Delivery.

PubMed

Morgalla, Matthias; Fortunato, Marcos; Azam, Ala; Tatagiba, Marcos; Lepski, Guillherme

2016-07-01

The assessment of the functionality of intrathecal drug delivery (IDD) systems remains difficult and time-consuming. Catheter-related problems are still very common, and sometimes difficult to diagnose. The aim of the present study is to investigate the accuracy of high-resolution three-dimensional computed tomography (CT) in order to detect catheter-related pump dysfunction. An observational, retrospective investigation. Academic medical center in Germany. We used high-resolution three dimensional (3D) computed tomography with volume rendering technique (VRT) or fluoroscopy and conventional axial-CT to assess IDD-related complications in 51 patients from our institution who had IDD systems implanted for the treatment of chronic pain or spasticity. Twelve patients (23.5%) presented a total of 22 complications. The main type of complication in our series was catheter-related (50%), followed by pump failure, infection, and inappropriate refilling. Fluoroscopy and conventional CT were used in 12 cases. High-resolution 3D CT VRT scan was used in 35 instances with suspected yet unclear complications. Using 3D-CT (VRT) the sensitivity was 58.93% - 100% (CI 95%) and the specificity 87.54% - 100% (CI 95%).The positive predictive value was 58.93% - 100% (CI 95%) and the negative predictive value: 87.54% - 100% (CI 95%).Fluoroscopy and axial CT as a combined diagnostic tool had a sensitivity of 8.3% - 91.7% (CI 95%) and a specificity of 62.9% - 100% (CI 95%). The positive predictive value was 19.29% - 100% (CI 95%) and the negative predictive value: 44.43% - 96.89% (CI 95%). This study is limited by its observational design and the small number of cases. High-resolution 3D CT VRT is a non- invasive method that can identify IDD-related complications with more precision than axial CT and fluoroscopy.

Thermospheric density variations: Observability using precision satellite orbits and effects on orbit propagation

NASA Astrophysics Data System (ADS)

Lechtenberg, Travis; McLaughlin, Craig A.; Locke, Travis; Krishna, Dhaval Mysore

2013-01-01

paper examines atmospheric density estimated using precision orbit ephemerides (POE) from the CHAMP and GRACE satellites during short periods of greater atmospheric density variability. The results of the calibration of CHAMP densities derived using POEs with those derived using accelerometers are examined for three different types of density perturbations, [traveling atmospheric disturbances (TADs), geomagnetic cusp phenomena, and midnight density maxima] in order to determine the temporal resolution of POE solutions. In addition, the densities are compared to High-Accuracy Satellite Drag Model (HASDM) densities to compare temporal resolution for both types of corrections. The resolution for these models of thermospheric density was found to be inadequate to sufficiently characterize the short-term density variations examined here. Also examined in this paper is the effect of differing density estimation schemes by propagating an initial orbit state forward in time and examining induced errors. The propagated POE-derived densities incurred errors of a smaller magnitude than the empirical models and errors on the same scale or better than those incurred using the HASDM model.

Debunking the Myth of Two-Temperature Coronae for RS CVn Systems Using Contemporaneous

NASA Astrophysics Data System (ADS)

Linsky, Jeffrey L.

For many years the standard analysis of low energy resolution x-ray observations of active late-type stars with the IPC, PSPC, TGS, and SSS has been to assume that the stellar coronae have plasma at only two temperatures. This type of analysis is constrained by the small information content and limited bandpass of the data but has NO PHYSICAL BASIS WHATSOEVER. We propose to test this hypothesis and to go beyond it to derive continuous emission measure distributions for the coronae of three very bright RS CVn systems (sigma-2 Cor Bor, UX Ari and VY Ari) using CONTEMPORANEOUS high resolution EUVE spectra and the improved x-ray energy resolution of ASCA. EUVE provides Fe lines with a broad range of ionization to derive the emission measure EM(T) independent of any uncertainties in the coronal abundances, while ASCA provides information on the hot plasma as seen in blended features of Mg, Si, S, and Fe and can test for coronal abundances different from the photosphere. We will model the quiescent and flare emission with magnetic loops.

Absolute parameters and chemical composition of the binary star OU Gem

NASA Astrophysics Data System (ADS)

Glazunova, L. V.; Mishenina, T. V.; Soubiran, C.; Kovtyukh, V. V.

2014-10-01

The absolute parameters and chemical composition of the BY Dra-type spectroscopic binary OU Gem (HD 45088) were determined on the basis of 10 high-resolution spectra. A new orbital solution of the binary system was determined, the binary ephemerides were specified, and the main physical and atmospheric parameters of the binary components were obtained. The chemical composition of both components was estimated for the first time for the stars of such type.

Diode laser slit-jet spectra and analysis of the fundamental of 1-chloro-1,1-difluoroethane (HCFC-142b)

NASA Astrophysics Data System (ADS)

Snels, M.; D'Amico, G.

2002-11-01

High resolution infrared spectra (0.001 cm^{-1}) have been measured for mixtures of 1-chloro-1,1-difluoroethane in Ne, expanded in a supersonic planar jet. The ν_7 fundamental has been analyzed for both isotopic species, CH3CF2 ^{35}Cl and CH3CF2 ^{37}Cl. A weak b-type component has been observed for the first time.

User Friendly Processing of Sediment CT Data: Software and Application in High Resolution Non-Destructive Sediment Core Data Sets

NASA Astrophysics Data System (ADS)

Reilly, B. T.; Stoner, J. S.; Wiest, J.; Abbott, M. B.; Francus, P.; Lapointe, F.

2015-12-01

Computed Tomography (CT) of sediment cores allow for high resolution images, three dimensional volumes, and down core profiles, generated through the attenuation of X-rays as a function of density and atomic number. When using a medical CT-Scanner, these quantitative data are stored in pixels using the Hounsfield scale, which are relative to the attenuation of X-rays in water and air at standard temperature and pressure. Here we present MATLAB based software specifically designed for sedimentary applications with a user friendly graphical interface to process DICOM files and stitch overlapping CT scans. For visualization, the software allows easy generation of core slice images with grayscale and false color relative to a user defined Hounsfield number range. For comparison to other high resolution non-destructive methods, down core Hounsfield number profiles are extracted using a method robust to coring imperfections, like deformation, bowing, gaps, and gas expansion. We demonstrate the usefulness of this technique with lacustrine sediment cores from the Western United States and Canadian High Arctic, including Fish Lake, Oregon, and Sawtooth Lake, Ellesmere Island. These sites represent two different depositional environments and provide examples for a variety of common coring defects and lithologies. The Hounsfield profiles and images can be used in combination with other high resolution data sets, including sediment magnetic parameters, XRF core scans and many other types of data, to provide unique insights into how lithology influences paleoenvironmental and paleomagnetic records and their interpretations.

Subcellular-level resolution MALDI-MS imaging of maize leaf metabolites by MALDI-linear ion trap-Orbitrap mass spectrometer

DOE PAGES

Korte, Andrew R.; Yandeau-Nelson, Marna D.; Nikolau, Basil J.; ...

2015-01-25

A significant limiting factor in achieving high spatial resolution for matrix-assisted laser desorption ionization-mass spectrometry (MALDI-MS) imaging is the size of the laser spot at the sample surface. We present modifications to the beam-delivery optics of a commercial MALDI-linear ion trap-Orbitrap instrument, incorporating an external Nd:YAG laser, beam-shaping optics, and an aspheric focusing lens, to reduce the minimum laser spot size from ~50 μm for the commercial configuration down to ~9 μm for the modified configuration. This improved system was applied for MALDI-MS imaging of cross sections of juvenile maize leaves at 5-μm spatial resolution using an oversampling method. Theremore » are a variety of different metabolites including amino acids, glycerolipids, and defense-related compounds were imaged at a spatial resolution well below the size of a single cell. Such images provide unprecedented insights into the metabolism associated with the different tissue types of the maize leaf, which is known to asymmetrically distribute the reactions of C4 photosynthesis among the mesophyll and bundle sheath cell types. The metabolite ion images correlate with the optical images that reveal the structures of the different tissues, and previously known and newly revealed asymmetric metabolic features are observed.« less

Pyrolysis-high resolution gas chromatography and pyrolysis gas chromatography-mass spectrometry of kerogens and kerogen precursors

NASA Technical Reports Server (NTRS)

Van De Meent, D.; Brown, S. C.; Philp, R. P.; Simoneit, B. R. T.

1980-01-01

A series of kerogens and kerogen precursors isolated from DSDP samples, oil shales and recent algal mats have been examined by Curie point pyrolysis-high resolution gas chromatography and gas chromatography-mass spectrometry. This study has shown that the three main types of kerogens (marine, terrestrial and mixtures of both) can be characterized using these techniques. The marine (algal) kerogens yield principally aliphatic products and the terrestrial kerogens yield more aromatic and phenolic products with some n-alkanes and n-alkenes. The yields of n-alkanes and n-alkenes increase and phenols decrease with increasing geologic age, however, pyrolysis-GC cannot be used to characterize the influence of short term diagenesis on the kerogen structure.

Redundancy management of multiple KT-70 inertial measurement units applicable to the space shuttle

NASA Technical Reports Server (NTRS)

Cook, L. J.

1975-01-01

Results of an investigation of velocity failure detection and isolation for 3 inertial measuring units (IMU) and 2 inertial measuring units (IMU) configurations are presented. The failure detection and isolation algorithm performance was highly successful and most types of velocity errors were detected and isolated. The failure detection and isolation algorithm also included attitude FDI but was not evaluated because of the lack of time and low resolution in the gimbal angle synchro outputs. The shuttle KT-70 IMUs will have dual-speed resolvers and high resolution gimbal angle readouts. It was demonstrated by these tests that a single computer utilizing a serial data bus can successfully control a redundant 3-IMU system and perform FDI.

High Resolution X-Ray Micro-CT of Ultra-Thin Wall Space Components

NASA Technical Reports Server (NTRS)

Roth, Don J.; Rauser, R. W.; Bowman, Randy R.; Bonacuse, Peter; Martin, Richard E.; Locci, I. E.; Kelley, M.

2012-01-01

A high resolution micro-CT system has been assembled and is being used to provide optimal characterization for ultra-thin wall space components. The Glenn Research Center NDE Sciences Team, using this CT system, has assumed the role of inspection vendor for the Advanced Stirling Convertor (ASC) project at NASA. This article will discuss many aspects of the development of the CT scanning for this type of component, including CT system overview; inspection requirements; process development, software utilized and developed to visualize, process, and analyze results; calibration sample development; results on actual samples; correlation with optical/SEM characterization; CT modeling; and development of automatic flaw recognition software. Keywords: Nondestructive Evaluation, NDE, Computed Tomography, Imaging, X-ray, Metallic Components, Thin Wall Inspection

Relaxation approximations to second-order traffic flow models by high-resolution schemes

DOE Office of Scientific and Technical Information (OSTI.GOV)

Nikolos, I.K.; Delis, A.I.; Papageorgiou, M.

2015-03-10

A relaxation-type approximation of second-order non-equilibrium traffic models, written in conservation or balance law form, is considered. Using the relaxation approximation, the nonlinear equations are transformed to a semi-linear diagonilizable problem with linear characteristic variables and stiff source terms with the attractive feature that neither Riemann solvers nor characteristic decompositions are in need. In particular, it is only necessary to provide the flux and source term functions and an estimate of the characteristic speeds. To discretize the resulting relaxation system, high-resolution reconstructions in space are considered. Emphasis is given on a fifth-order WENO scheme and its performance. The computations reportedmore » demonstrate the simplicity and versatility of relaxation schemes as numerical solvers.« less

Analytical ultrasonics for evaluation of composite materials response. Part 2: Generation and detection

NASA Technical Reports Server (NTRS)

Duke, J. C., Jr.; Henneke, E. G., II

1986-01-01

To evaluate the response of composite materials, it is imperative that the input excitation as well as the observed output be well characterized. This characterization ideally should be in terms of displacements as a function of time with high spatial resolution. Additionally, the ability to prescribe these features for the excitation is highly desirable. Various methods for generating and detecting ultrasound in advanced composite materials are examined. Characterization and tailoring of input excitation is considered for contact and noncontact, mechanical, and electromechanical devices. Type of response as well as temporal and spatial resolution of detection methods are discussed as well. Results of investigations at Virginia Tech in application of these techniques to characterizing the response of advanced composites are presented.

Diagnosis and Treatment Strategy of Achalasia Subtypes and Esophagogastric Junction Outflow Obstruction Based on High-Resolution Manometry.

PubMed

Ihara, Eikichi; Muta, Kazumasa; Fukaura, Keita; Nakamura, Kazuhiko

2017-01-01

Based on Chicago Classification version 3.0, the disorders of esophagogastric junction outflow obstruction (EGJOO) include achalasia (types I, II and III) and EGJOO. Although no curative treatments are currently available for the treatment of the disorders of EGJOO, medical treatments, endoscopic pneumatic dilation (PD), laparoscopic Heller myotomy (LHM), and per-oral endoscopic myotomy (POEM) are usually the sought-after modes of treatment. Since the etiology and pathogenesis might vary depending on the types of EGJOO disorders, treatment strategies should be considered based on those subtypes. Based on the accumulated evidences, the treatment strategies of our institution are as follows: effects of medical treatments on achalasia are limited. Either PD or LHM/POEM can be considered a first-line in achalasia type I, according to the patient's wish. PD and POEM can be considered first-line in achalasia types II and III, respectively. Conversely, In EGJOO, medical treatments including drugs like acotiamide and/or diltiazem can be tested as a first-line, and PD and POEM will be considered second and third-line treatments, respectively. Key Messages: The classification of subtypes based on high-resolution manometry will help us consider which treatment option can be selected as a first-line treatment depending upon the subtypes of disorders of EGJOO. Acotiamide has the potential to cure patients with EGJOO. © 2016 S. Karger AG, Basel.

[Prediction of round window visibility in cochlear implantation with temporal bone high resolution computed tomography].

PubMed

Sun, S P; Lu, W; Lei, Y B; Men, X M; Zuo, B; Ding, S G

2017-08-07

Objective: To discuss the prediction of round window(RW) visibility in cochlear implantation(CI) with temporal bone high resolution computed tomography(HRCT). Methods: From January 2013 to January 2017, 130 cases underwent both HRCT and CI in our hospital were analyzed. The distance from facial nerve to posterior canal wall(FWD), the angle between facial nerve and inner margin of round window(FRA), and the angle between facial nerve and tympanic anulus to inner margin of round window(FRAA) were detected at the level of round window on axial temporal bone HRCT. A line parallel to the posterior wall of ear canal was drawn from the anterior wall of facial nerve at the level of round window on axial temporal bone HRCT and its relationship with round window was detected (facial-round window line, FRL): type0-posterior to the round window, type1-between the round window, type2-anterior to the round window. Their(FWD, FRA, FRAA, FRL) relationships with intra-operative round window visibility were analyzed by SPSS 17.0 software. Results: FWD( F =18.76, P =0.00), FRA( F =34.57, P =0.00), FRAA ( F =14.24, P =0.00) could affect the intra-operative RW visibility significantly. RW could be exposed completely during CI when preoperative HRCT showing type0 FRL. RW might be partly exposed and not exposed when preoperative HRCT showing type1 and type2 FRL respectively. Conclusion: FWD, FRA, FRAA and FRL of temporal bone HRCT can predict intra-operative round window visibility effectively in CI surgery.

Surface water classification and monitoring using polarimetric synthetic aperture radar

NASA Astrophysics Data System (ADS)

Irwin, Katherine Elizabeth

Surface water classification using synthetic aperture radar (SAR) is an established practice for monitoring flood hazards due to the high temporal and spatial resolution it provides. Surface water change is a dynamic process that varies both spatially and temporally, and can occur on various scales resulting in significant impacts on affected areas. Small-scale flooding hazards, caused by beaver dam failure, is an example of surface water change, which can impact nearby infrastructure and ecosystems. Assessing these hazards is essential to transportation and infrastructure maintenance. With current satellite missions operating in multiple polarizations, spatio-temporal resolutions, and frequencies, a comprehensive comparison between SAR products for surface water monitoring is necessary. In this thesis, surface water extent models derived from high resolution single-polarization TerraSAR-X (TSX) data, medium resolution dual-polarization TSX data and low resolution quad-polarization RADARSAT-2 (RS-2) data are compared. There exists a compromise between acquiring SAR data with a high resolution or high information content. Multi-polarization data provides additional phase and intensity information, which makes it possible to better classify areas of flooded vegetation and wetlands. These locations are often where fluctuations in surface water occur and are essential for understanding dynamic underlying processes. However, often multi-polarized data is acquired at a low resolution, which cannot image these zones effectively. High spatial resolution, single-polarization TSX data provides the best model of open water. However, these single-polarization observations have limited information content and are affected by shadow and layover errors. This often hinders the classification of other land cover types. The dual-polarization TSX data allows for the classification of flooded vegetation, but classification is less accurate compared to the quad-polarization RS-2 data. The RS-2 data allows for the discrimination of open water, marshes/fields and forested areas. However, the RS-2 data is less applicable to small scale surface water monitoring (e.g. beaver dam failure), due to its low spatial resolution. By understanding the strengths and weaknesses of available SAR technology, an appropriate product can be chosen for a specific target application involving surface water change. This research benefits the eventual development of a space-based monitoring strategy over longer periods.

Influences of Altered River Geomorphology on Channel-Floodplain Mass and Momentum Transfer

NASA Astrophysics Data System (ADS)

Byrne, C. F.; Stone, M. C.

2017-12-01

River management strategies, including both river engineering and restoration, have altered river geomorphology and associated lateral channel-floodplain connectivity throughout the world. This altered connectivity is known to drive changes in ecologic and geomorphic processes during floods, however, quantification of altered connectivity is difficult due to the highly dynamic spatial and temporal nature of flood wave conditions. The objective of this research was to quantify the physical processes of lateral mass and momentum transfer at the channel-floodplain interface. The objective was achieved with the implementation of novel scripting and high-resolution, two-dimensional hydrodynamic modeling techniques under unsteady flow conditions. The process-based analysis focused on three geomorphic feature types within the Middle Rio Grande, New Mexico, USA: (1) historical floodplain surfaces, (2) inset floodplain surfaces formed as a result of channel training and hydrologic alteration, and (3) mechanically restored floodplain surfaces. Results suggest that inset floodplain feature types are not only subject to greater mass and momentum transfer magnitudes, but those connections are also more heterogeneous in nature compared with historical feature types. While restored floodplain feature types exhibit transfer magnitudes and heterogeneity comparable to inset feature types, the surfaces are not of great enough spatial extent to substantially influence total channel-floodplain mass and momentum transfer. Mass and momentum transfer also displayed differing characteristic changes as a result of increased flood magnitude, indicating that linked hydrodynamic processes can be altered differently as a result of geomorphic and hydrologic change. The results display the potential of high-resolution modeling strategies in capturing the spatial and temporal complexities of river processes. In addition, the results have implications for other fields of river science including biogeochemical exchange at the channel-floodplain interface and quantification of process associated with environmental flow and river restoration strategies.

High resolution 3D confocal microscope imaging of volcanic ash particles.

PubMed

Wertheim, David; Gillmore, Gavin; Gill, Ian; Petford, Nick

2017-07-15

We present initial results from a novel high resolution confocal microscopy study of the 3D surface structure of volcanic ash particles from two recent explosive basaltic eruptions, Eyjafjallajökull (2010) and Grimsvötn (2011), in Iceland. The majority of particles imaged are less than 100μm in size and include PM 10 s, known to be harmful to humans if inhaled. Previous studies have mainly used 2D microscopy to examine volcanic particles. The aim of this study was to test the potential of 3D laser scanning confocal microscopy as a reliable analysis tool for these materials and if so to what degree high resolution surface and volume data could be obtained that would further aid in their classification. First results obtained using an Olympus LEXT scanning confocal microscope with a ×50 and ×100 objective lens are highly encouraging. They reveal a range of discrete particle types characterised by sharp or concave edges consistent with explosive formation and sudden rupture of magma. Initial surface area/volume ratios are given that may prove useful in subsequent modelling of damage to aircraft engines and human tissue where inhalation has occurred. Copyright © 2017 Elsevier B.V. All rights reserved.

Millimeter and Sub-millimeter High Resolution Spectroscopy: New Frontiers with ALMA

NASA Astrophysics Data System (ADS)

Ziurys, Lucy M.

2016-06-01

It is becoming increasingly clear that new laboratory data will be critical for the next decade of observations with the Atacama Large Millimeter Array (ALMA). The high spatial resolution offered by ALMA will probe new regions of molecular complexity, including the inner envelopes of evolved stars, regions dominated by UV radiation, and the densest cores of molecular clouds. New molecular lines will be discovered in the wide wavelength range covered by the ALMA bands, and high resolution, gas-phase spectroscopy are needed to provide crucial “rest frequencies.” In particular, highly accurate methods that measure millimeter and sub-millimeter rotational transitions, such as direct absorption and Fourier transform mm-wave techniques, are important, especially when coupled to exotic molecular production schemes. Recent ALMA studies of SH+ and larger organic species have already demonstrated the need for laboratory measurements. New laboratory work will likely be required for circumstellar refractory molecules, radicals and ions generated near photon-dominated regions (PDRs), and large, organic-type species. This talk will give an overview of current contributions of laboratory spectroscopy to ALMA observations, summarize relevant spectroscopic techniques, and provide input into future prospects and directions.

Edible seaweed as future functional food: Identification of α-glucosidase inhibitors by combined use of high-resolution α-glucosidase inhibition profiling and HPLC-HRMS-SPE-NMR.

PubMed

Liu, Bingrui; Kongstad, Kenneth T; Wiese, Stefanie; Jäger, Anna K; Staerk, Dan

2016-07-15

Crude chloroform, ethanol and acetone extracts of nineteen seaweed species were screened for their antioxidant and α-glucosidase inhibitory activity. Samples showing more than 60% α-glucosidase inhibitory activity, at a concentration of 1 mg/ml, were furthermore investigated using high-resolution α-glucosidase inhibition profiling combined with high-performance liquid chromatography-high-resolution mass spectrometry-solid-phase extraction-nuclear magnetic resonance spectroscopy (HR-bioassay/HPLC-HRMS-SPE-NMR). The results showed Ascophyllum nodosum and Fucus vesicolosus to be rich in antioxidants, equaling a Trolox equivalent antioxidant capacity of 135 and 108 mM Troloxmg(-1) extract, respectively. HR-bioassay/HPLC-HRMS-SPE-NMR showed the α-glucosidase inhibitory activity of A. nodosum, F. vesoculosus, Laminaria digitata, Laminaria japonica and Undaria pinnatifida to be caused by phlorotannins as well as fatty acids - with oleic acid, linoleic acid and eicosapentaenoic acid being the most potent with IC50 values of 0.069, 0.075 and 0.10 mM, respectively, and showing a mixed-type inhibition mode. Copyright © 2016 Elsevier Ltd. All rights reserved.

Velocity map imaging using an in-vacuum pixel detector

DOE Office of Scientific and Technical Information (OSTI.GOV)

Gademann, Georg; Huismans, Ymkje; Gijsbertsen, Arjan

The use of a new type in-vacuum pixel detector in velocity map imaging (VMI) is introduced. The Medipix2 and Timepix semiconductor pixel detectors (256x256 square pixels, 55x55 {mu}m{sup 2}) are well suited for charged particle detection. They offer high resolution, low noise, and high quantum efficiency. The Medipix2 chip allows double energy discrimination by offering a low and a high energy threshold. The Timepix detector allows to record the incidence time of a particle with a temporal resolution of 10 ns and a dynamic range of 160 {mu}s. Results of the first time application of the Medipix2 detector to VMImore » are presented, investigating the quantum efficiency as well as the possibility to operate at increased background pressure in the vacuum chamber.« less

Oxidized crystalline (3 × 1)-O surface phases of InAs and InSb studied by high-resolution photoelectron spectroscopy

DOE Office of Scientific and Technical Information (OSTI.GOV)

Tuominen, M., E-mail: tmleir@utu.fi, E-mail: pekka.laukkanen@utu.fi; Lång, J.; Dahl, J.

2015-01-05

The pre-oxidized crystalline (3×1)-O structure of InAs(100) has been recently found to significantly improve insulator/InAs junctions for devices, but the atomic structure and formation of this useful oxide layer are not well understood. We report high-resolution photoelectron spectroscopy analysis of (3×1)-O on InAs(100) and InSb(100). The findings reveal that the atomic structure of (3×1)-O consists of In atoms with unexpected negative (between −0.64 and −0.47 eV) and only moderate positive (In{sub 2}O type) core-level shifts; highly oxidized group-V sites; and four different oxygen sites. These fingerprint shifts are compared to those of previously studied oxides of III-V to elucidate oxidation processes.

The Environmental Impact of Intra-Cluster Medium on the Interstellar Medium in Early Type Galaxies

NASA Technical Reports Server (NTRS)

Trinchieri, Ginevra

1993-01-01

Draft versions of three articles submitted for publication are presented. The first two articles address high resolution X-ray images of early type galaxies observed with the ROSAT HRI and PSPC. Data for NGC 1553 and NGC 5846 indicate that the emission is highly irregular, with interesting features at different scales. The gas temperatures also vary both with the galactocentric radius and in correspondence to regions of higher emission and denser material. Strikingly similar features are observed in the X-ray and H-alpha morphologies of NGC 1553 and NGC 5846, while smooth, regular isophotes are observed in NGC 4649 at both wavelengths. The third article addresses ROSAT PSPC observations of 5 X-ray bright early type galaxies.

Highly-evolved stars

NASA Technical Reports Server (NTRS)

Heap, S. R.

1981-01-01

The ways in which the IUE has proved useful in studying highly evolved stars are reviewed. The importance of high dispersion spectra for abundance analyses of the sd0 stars and for studies of the wind from the central star of NGC 6543 and the wind from the 0 type component of Vela X-1 is shown. Low dispersion spectra are used for absolute spectrophotometry of the dwarf nova, Ex Hya. Angular resolution is important for detecting and locating UV sources in globular clusters.

Observation and simulation of net primary productivity in Qilian Mountain, western China.

PubMed

Zhou, Y; Zhu, Q; Chen, J M; Wang, Y Q; Liu, J; Sun, R; Tang, S

2007-11-01

We modeled net primary productivity (NPP) at high spatial resolution using an advanced spaceborne thermal emission and reflection radiometer (ASTER) image of a Qilian Mountain study area using the boreal ecosystem productivity simulator (BEPS). Two key driving variables of the model, leaf area index (LAI) and land cover type, were derived from ASTER and moderate resolution imaging spectroradiometer (MODIS) data. Other spatially explicit inputs included daily meteorological data (radiation, precipitation, temperature, humidity), available soil water holding capacity (AWC), and forest biomass. NPP was estimated for coniferous forests and other land cover types in the study area. The result showed that NPP of coniferous forests in the study area was about 4.4 tCha(-1)y(-1). The correlation coefficient between the modeled NPP and ground measurements was 0.84, with a mean relative error of about 13.9%.

Retrieval of methanol absorption parameters at terahertz frequencies using multispectral fitting

NASA Astrophysics Data System (ADS)

Slocum, David M.; Xu, Li-Hong; Giles, Robert H.; Goyette, Thomas M.

2015-12-01

A high-resolution broadband study of the methanol absorption spectrum was performed at 1.480-1.495 THz. The transmittance was recorded under both self- and air-broadening conditions for multiple pressures at a resolution of 500 kHz. A multispectral fitting analysis was then performed. The transition frequency, absolute intensity, self- and air-broadening coefficients, and self- and air-induced pressure shifts were retrieved for 221 absorption lines using the multispectral fitting routine. Observed in the data were two different series of transitions, both a b-type Q-branch with K = - 7 ← - 6 and an a-type R-branch with J = 31 ← 30 . The retrieved frequency position values were compared with values from spectral databases and trends within the different series were identified. An analysis of the precision of the fitting routine was also performed.

Common path in-line holography using enhanced joint object reference digital interferometers

PubMed Central

Kelner, Roy; Katz, Barak; Rosen, Joseph

2014-01-01

Joint object reference digital interferometer (JORDI) is a recently developed system capable of recording holograms of various types [Opt. Lett. 38(22), 4719 (2013)24322115]. Presented here is a new enhanced system design that is based on the previous JORDI. While the previous JORDI has been based purely on diffractive optical elements, displayed on spatial light modulators, the present design incorporates an additional refractive objective lens, thus enabling hologram recording with improved resolution and increased system applicability. Experimental results demonstrate successful hologram recording for various types of objects, including transmissive, reflective, three-dimensional, phase and highly scattering objects. The resolution limit of the system is analyzed and experimentally validated. Finally, the suitability of JORDI for microscopic applications is verified as a microscope objective based configuration of the system is demonstrated. PMID:24663838

An integrated signal conditioner for high-frequency inductive position sensors

NASA Astrophysics Data System (ADS)

Rahal, Mohamad; Demosthenous, Andreas

2010-01-01

This paper describes the design, implementation and evaluation of a signal conditioner application-specific integrated circuit (ASIC) for high-frequency inductive non-contact position sensors. These sensors employ a radio frequency technology based on an antenna planar arrangement and a resonant target, have a high inherent resolution (0.1% of antenna length) and can measure target position over a wide distance range (<0.1 mm to >10 m). However, due to the relatively high-frequency excitation (1 MHz typically) and to the specific layouts of these sensors, there is unwanted capacitive coupling between the transmitter and receiver coils; this type of distortion reduces linearity and resolution. The ASIC, which is the first generation of its kind for this type of sensor, employs a differential mixer topology which suppresses the capacitive coupling offsets. The system architecture and circuit details are presented. The ASIC was fabricated in a 0.6 µm high-voltage CMOS technology occupying an area of 8 mm2. It dissipates about 30 mA from a 24 V power supply. The ASIC was tested with a high-frequency inductive position sensor (with an antenna length of 10.8 cm). The measured input-referred offset due to transmitter crosstalk is on average about 22 µV over a wide phase difference variation (-99° to +117°) between the transmitter and demodulating signals.

On the 3He anomaly in hot subdwarf B stars

NASA Astrophysics Data System (ADS)

Schneider, David; Irrgang, Andreas; Heber, Ulrich; Nieva, Maria F.; Przybilla, Norbert

2017-12-01

Decades ago, 3He isotope enrichment in helium-weak B-type main-sequence, in blue horizontal branch and in hot subdwarf B (sdB) stars, i.e., helium-core burning stars of the extreme horizontal branch, were discovered. Diffusion processes in the atmosphere of these stars lead to the observed abundance anomalies. Quantitative spectral analyses of high-resolution spectra to derive photospheric isotopic helium abundance ratios for known 3He sdBs have not been performed yet. We present preliminary results of high-resolution and high S/N spectra to determine the 3He and 4He abundances of nine known 3He sdBs. We used a hybrid local/non-local thermodynamic equilibrium (LTE/NLTE) approach for B-type stars investigating multiple He i lines, including λ4922 Å and λ6678 Å, which show the strongest isotopic shifts in the optical spectral range.We also report the discovery of four new 3He sdBs from the ESO Supernova Progenitor survey. Most of the 3He sdBs cluster in a narrow temperature strip between ˜ 26000 K and ˜ 30000 K and have almost no atmospheric 4He at all. Interestingly, three 3He sdBs show evidence for vertical helium stratification.

High-Resolution Analysis of the ν 6, ν 7, ν 8, and ν 9Bands of H 15N 16O 3Measured by Fourier Transform Spectroscopy

NASA Astrophysics Data System (ADS)

Keller, F.; Perrin, A.; Flaud, J.-M.; Johns, J. W. C.; Lu, Z.; Looi, E. C.

1998-10-01

The analysis of the ν6, ν7, ν8, and ν9bands of H15N16O3located at 646.9641, 578.4719, 743.6166, and 458.2917 cm-1, respectively, has been carried out in the 400-800 cm-1region using high-resolution Fourier transform spectra recorded at Ottawa. Using the ground state energy levels calculated from thev= 0 rotational constants of H15N16O3[A. P. Cox, M. C. Ellis, C. J. Attfield, and A. C. Ferris,J. Mol. Struct.320, 91-106 (1994)], it was possible to assign theA-type ν6and ν7bands and theC-type ν8and ν9bands of H15N16O3up to highJandKarotational quantum numbers. Thev6= 1,v7= 1, v8= 1, andv9= 1 experimental energy levels were then introduced in a least-squares fit calculation and precise upper state Hamiltonian constants (band centers and rotational constants) were determined allowing one to reproduce the infrared data to within the experimental uncertainty.

Machine Learning Based Single-Frame Super-Resolution Processing for Lensless Blood Cell Counting

PubMed Central

Huang, Xiwei; Jiang, Yu; Liu, Xu; Xu, Hang; Han, Zhi; Rong, Hailong; Yang, Haiping; Yan, Mei; Yu, Hao

2016-01-01

A lensless blood cell counting system integrating microfluidic channel and a complementary metal oxide semiconductor (CMOS) image sensor is a promising technique to miniaturize the conventional optical lens based imaging system for point-of-care testing (POCT). However, such a system has limited resolution, making it imperative to improve resolution from the system-level using super-resolution (SR) processing. Yet, how to improve resolution towards better cell detection and recognition with low cost of processing resources and without degrading system throughput is still a challenge. In this article, two machine learning based single-frame SR processing types are proposed and compared for lensless blood cell counting, namely the Extreme Learning Machine based SR (ELMSR) and Convolutional Neural Network based SR (CNNSR). Moreover, lensless blood cell counting prototypes using commercial CMOS image sensors and custom designed backside-illuminated CMOS image sensors are demonstrated with ELMSR and CNNSR. When one captured low-resolution lensless cell image is input, an improved high-resolution cell image will be output. The experimental results show that the cell resolution is improved by 4×, and CNNSR has 9.5% improvement over the ELMSR on resolution enhancing performance. The cell counting results also match well with a commercial flow cytometer. Such ELMSR and CNNSR therefore have the potential for efficient resolution improvement in lensless blood cell counting systems towards POCT applications. PMID:27827837

Using Power Spectrum Analysis to Evaluate 18O-Water Labeling Data Acquired from Low Resolution Mass Spectrometers

PubMed Central

Sadygov, Rovshan G.; Zhao, Yingxin; Haidacher, Sigmund J.; Starkey, Jonathan M.; Tilton, Ronald G.; Denner, Larry

2010-01-01

We describe a method for ratio estimations in 18O-water labeling experiments acquired from low resolution isotopically resolved data. The method is implemented in a software package specifically designed for use in experiments making use of zoom-scan mode data acquisition. Zoom-scan mode data allows commonly used ion trap mass spectrometers to attain isotopic resolution, which make them amenable to use in labeling schemes such as 18O-water labeling, but algorithms and software developed for high resolution instruments may not be appropriate for the lower resolution data acquired in zoom-scan mode. The use of power spectrum analysis is proposed as a general approach which may be uniquely suited to these data types. The software implementation uses power spectrum to remove high-frequency noise, and band-filter contributions from co-eluting species of differing charge states. From the elemental composition of a peptide sequence we generate theoretical isotope envelopes of heavy-light peptide pairs in five different ratios; these theoretical envelopes are correlated with the filtered experimental zoom scans. To automate peptide quantification in high-throughput experiments, we have implemented our approach in a computer program, MassXplorer. We demonstrate the application of MassXplorer to two model mixtures of known proteins, and to a complex mixture of mouse kidney cortical extract. Comparison with another algorithm for ratio estimations demonstrates the increased precision and automation of MassXplorer. PMID:20568695