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Sample records for high-resolution optical recording

  1. High-resolution non-contact measurement of the electrical activity of plants in situ using optical recording

    PubMed Central

    Zhao, Dong-Jie; Chen, Yang; Wang, Zi-Yang; Xue, Lin; Mao, Tong-Lin; Liu, Yi-Min; Wang, Zhong-Yi; Huang, Lan

    2015-01-01

    The limitations of conventional extracellular recording and intracellular recording make high-resolution multisite recording of plant bioelectrical activity in situ challenging. By combining a cooled charge-coupled device camera with a voltage-sensitive dye, we recorded the action potentials in the stem of Helianthus annuus and variation potentials at multiple sites simultaneously with high spatial resolution. The method of signal processing using coherence analysis was used to determine the synchronization of the selected signals. Our results provide direct visualization of the phloem, which is the distribution region of the electrical activities in the stem and leaf of H. annuus, and verify that the phloem is the main action potential transmission route in the stems of higher plants. Finally, the method of optical recording offers a unique opportunity to map the dynamic bioelectrical activity and provides an insight into the mechanisms of long-distance electrical signal transmission in higher plants. PMID:26333536

  2. Holographic high-resolution endoscopic image recording

    NASA Astrophysics Data System (ADS)

    Bjelkhagen, Hans I.

    1991-03-01

    Endoscopic holography or endoholography combines the features of endoscopy and holography. The purpose of endoholographic imaging is to provide the physician with a unique means of extending diagnosis by providing a life-like record of tissue. Endoholographic recording will provide means for microscopic examination of tissue and in some cases may obviate the need to excise specimens for biopsy. In this method holograms which have the unique properties of three-dimensionality large focal depth and high resolution are made with a newly designed endoscope. The endoscope uses a single-mode optical fiber for illumination and single-beam reflection holograms are recorded in close contact with the tissue at the distal end of the endoscope. The holograms are viewed under a microscope. By using the proper combinations of dyes for staining specific tissue types with various wavelengths of laser illumination increased contrast on the cellular level can be obtained. Using dyes such as rose bengal in combination with the 514. 5 nm line of an argon ion laser and trypan blue or methylene blue with the 647. 1 nm line of a krypton ion laser holograms of the stained colon of a dog showed the architecture of the colon''s columnar epithelial cells. It is hoped through chronological study using this method in-vivo an increased understanding of the etiology and pathology of diseases such as Crohn''s diseases colitis proctitis and several different forms of cancer will help

  3. High resolution optical DNA mapping

    NASA Astrophysics Data System (ADS)

    Baday, Murat

    Many types of diseases including cancer and autism are associated with copy-number variations in the genome. Most of these variations could not be identified with existing sequencing and optical DNA mapping methods. We have developed Multi-color Super-resolution technique, with potential for high throughput and low cost, which can allow us to recognize more of these variations. Our technique has made 10--fold improvement in the resolution of optical DNA mapping. Using a 180 kb BAC clone as a model system, we resolved dense patterns from 108 fluorescent labels of two different colors representing two different sequence-motifs. Overall, a detailed DNA map with 100 bp resolution was achieved, which has the potential to reveal detailed information about genetic variance and to facilitate medical diagnosis of genetic disease.

  4. High resolution wavefront measurement of aspheric optics

    NASA Astrophysics Data System (ADS)

    Erichsen, I.; Krey, S.; Heinisch, J.; Ruprecht, A.; Dumitrescu, E.

    2008-08-01

    With the recently emerged large volume production of miniature aspheric lenses for a wide range of applications, a new fast fully automatic high resolution wavefront measurement instrument has been developed. The Shack-Hartmann based system with reproducibility better than 0.05 waves is able to measure highly aspheric optics and allows for real time comparison with design data. Integrated advanced analysis tools such as calculation of Zernike coefficients, 2D-Modulation Transfer Function (MTF), Point Spread Function (PSF), Strehl-Ratio and the measurement of effective focal length (EFL) as well as flange focal length (FFL) allow for the direct verification of lens properties and can be used in a development as well as in a production environment.

  5. Wavefront metrology for high resolution optical systems

    NASA Astrophysics Data System (ADS)

    Miyakawa, Ryan H.

    Next generation extreme ultraviolet (EUV) optical systems are moving to higher resolution optics to accommodate smaller length scales targeted by the semiconductor industry. As the numerical apertures (NA) of the optics become larger, it becomes increasingly difficult to characterize aberrations due to experimental challenges associated with high-resolution spatial filters and geometrical effects caused by large incident angles of the test wavefront. This dissertation focuses on two methods of wavefront metrology for high resolution optical systems. The first method, lateral shearing interferometry (LSI), is a self-referencing interferometry where the test wavefront is incident on a low spatial frequency grating, and the resulting interference between the diffracted orders is used to reconstruct the wavefront aberrations. LSI has many advantages over other interferometric tests such as phase-shifting point diffraction interferometry (PS/PDI) due to its experimental simplicity, stability, relaxed coherence requirements, and its ability to scale to high numerical apertures. While LSI has historically been a qualitative test, this dissertation presents a novel quantitative investigation of the LSI interferogram. The analysis reveals the existence of systematic aberrations due to the nonlinear angular response from the diffraction grating that compromises the accuracy of LSI at medium to high NAs. In the medium NA regime (0.15 < NA < 0.35), a holographic model is presented that derives the systematic aberrations in closed form, which demonstrates an astigmatism term that scales as the square of the grating defocus. In the high NA regime (0.35 < NA), a geometrical model is introduced that describes the aberrations as a system of transcendental equations that can be solved numerically. The characterization and removal of these systematic errors is a necessary step that unlocks LSI as a viable candidate for high NA EUV optical testing. The second method is a novel image

  6. Wide swath and high resolution optical imaging satellite of Japan

    NASA Astrophysics Data System (ADS)

    Katayama, Haruyoshi; Kato, Eri; Imai, Hiroko; Sagisaka, Masakazu

    2016-05-01

    The "Advanced optical satellite" (tentative name) is a follow-on mission from ALOS. Mission objectives of the advanced optical satellite is to build upon the existing advanced techniques for global land observation using optical sensors, as well as to promote data utilization for social needs. Wide swath and high resolution optical imager onboard the advanced optical satellite will extend the capabilities of earlier ALOS missions. The optical imager will be able to collect high-resolution (< 1 m) and wide-swath (70 km) images with high geo-location accuracy. This paper introduces a conceptual design of the advanced optical satellite.

  7. Optical diffraction tomography for high resolution live cell imaging.

    PubMed

    Sung, Yongjin; Choi, Wonshik; Fang-Yen, Christopher; Badizadegan, Kamran; Dasari, Ramachandra R; Feld, Michael S

    2009-01-05

    We report the experimental implementation of optical diffraction tomography for quantitative 3D mapping of refractive index in live biological cells. Using a heterodyne Mach-Zehnder interferometer, we record complex field images of light transmitted through a sample with varying directions of illumination. To quantitatively reconstruct the 3D map of complex refractive index in live cells, we apply optical diffraction tomography based on the Rytov approximation. In this way, the effect of diffraction is taken into account in the reconstruction process and diffraction-free high resolution 3D images are obtained throughout the entire sample volume. The quantitative refractive index map can potentially serve as an intrinsic assay to provide the molecular concentrations without the addition of exogenous agents and also to provide a method for studying the light scattering properties of single cells.

  8. High Resolution Optical Imaging through the Atmosphere

    DTIC Science & Technology

    1989-12-28

    Papaliolios, with respect to the Sanduleak B3 supergiant At the time of Nisenson, and Ebstein 1985) which determines the x-y posi- these observations, it was...blown away by the SN flash. However, such a also wish to thank N. Carleton and S. Ebstein for their aid in source would be nearly the brightest star in...Papaliolios, Nisenson, and Ebstein 1986) and a front- speckle techniques, produced supporting evidence for the end optics package. The optics package includes

  9. Invariant high resolution optical skin imaging

    NASA Astrophysics Data System (ADS)

    Murali, Supraja; Rolland, Jannick

    2007-02-01

    Optical Coherence Microscopy (OCM) is a bio-medical low coherence interferometric imaging technique that has become a topic of active research because of its ability to provide accurate, non-invasive cross-sectional images of biological tissue with much greater resolution than the current common technique ultrasound. OCM is a derivative of Optical Coherence Tomography (OCT) that enables greater resolution imposed by the implementation of an optical confocal design involving high numerical aperture (NA) focusing in the sample. The primary setback of OCM, however is the depth dependence of the lateral resolution obtained that arises from the smaller depth of focus of the high NA beam. We propose to overcome this limitation using a dynamic focusing lens design that can achieve quasi-invariant lateral resolution up to 1.5mm depth of skin tissue.

  10. Optical autofocus for high resolution laser photoplotting

    NASA Astrophysics Data System (ADS)

    Alonso, Jose; Crespo, Daniel; Jimenez, Isidoro; Bernabeu, Eusebio

    2005-07-01

    An all optical autofocus has been designed and tested for tight line width control in a high NA laser photoplotter system. The laser system is based in a GaN semiconductor laser with power 30 mW and wavelength 405 nm. The advantage of using this laser, despite the relatively long wavenlength, is compactness and easy for high frequency modulation. The autofocus system is based in a secondary 635 nm GaAlAs laser without need for wavelength, neither power stabilization. The two beams are delivered coaxially through the focusing lens by means of a dichroic beamsplitter. Focusing lens need no correction for chromatic aberration, as this is compensed by appropriate autofocus beam divergence. After reflection in the sample, the autofocus beam is separated from the returning writing beam and then guided to a collimation sensor, in which defocus of about 1/20 of the Rayleigh range of the writing beam can be detected and compensated by an analogue PID electronic control. Stable linewidth within 5% is achieved with different numerical aperture focusing lenses.

  11. Compact and high-resolution optical orbital angular momentum sorter

    NASA Astrophysics Data System (ADS)

    Wan, Chenhao; Chen, Jian; Zhan, Qiwen

    2017-03-01

    A compact and high-resolution optical orbital angular momentum (OAM) sorter is proposed and demonstrated. The sorter comprises a quadratic fan-out mapper and a dual-phase corrector positioned in the pupil plane and the Fourier plane, respectively. The optical system is greatly simplified compared to previous demonstrations of OAM sorting, and the performance in resolution and efficiency is maintained. A folded configuration is set up using a single reflective spatial light modulator (SLM) to demonstrate the validity of the scheme. The two phase elements are implemented on the left and right halves of the SLM and connected by a right-angle prism. Experimental results demonstrate the high resolution of the compact OAM sorter, and the current limit in efficiency can be overcome by replacing with transmissive SLMs and removing the beam splitters. This novel scheme paves the way for the miniaturization and integration of high-resolution OAM sorters.

  12. Optical multichannel analyzer techniques for high resolution optical spectroscopy

    SciTech Connect

    Chao, J.L.

    1980-06-01

    The development of optical multichannel analyzer techniques for UV/VIS spectroscopy is presented. The research focuses on the development of spectroscopic techniques for measuring high resolution spectral lineshape functions from the exciton phosphorescence in H/sub 2/-1,2,4,5-tetrachlorobenzene. It is found that the temperature dependent frequency shifts and widths confirm a theoretical model based on an exchange theory. The exchange of low energy phonon modes which couple with excited state exciton transitions is shown to display the proper temperature dependent behavior. In addition to the techniques for using the optical multichannel analyzer (OMA) to perform low light level target integration, the use of the OMA for capturing spectral information in transient pulsed laser applications is discussed. An OMP data acquisition system developed for real-time signal processng is described. Both hardware and software interfacing considerations for control and data acquisition by a microcomputer are described. The OMA detector is described in terms of the principles behind its photoelectron detection capabilities and its design is compared with other optoelectronic devices.

  13. High-resolution electronic imaging system for schlieren recording

    NASA Astrophysics Data System (ADS)

    Honour, Joseph

    2001-04-01

    High speed Schlieren photography is a reliable means of visualizing small changes of refractive index resulting from density differences within a transparent media. Schlieren techniques are frequently used for investigating the aerodynamics of high velocity projectiles to confirm the formation of shock waves on leading edge surfaces so that optimum design performance can be achieved. Traditionally this type of investigation would have been undertaken using film cameras, however, improvements in image quality provided by the rapid development of intensified silicon based sensors and associated electronics has offered a reliable alternative, without the inherent difficulties in quantitative data extraction. The development of a high resolution sixteen image electronic camera system provides the researcher with versatile recording system that can be used to capture detailed image sequences at framing rates up to two hundred million pictures per second. The number of information points is maintained, irrespective of framing rate, making it ideal for recording the complexity of detail available from these sensitive Schlieren techniques. The high resolution images, which are displayed within twenty seconds of capture, flexibility of operation, and comprehensive analysis software provide fast reliable access to experimental data.

  14. Adaptive optics high resolution spectroscopy: present status and future direction

    SciTech Connect

    Alcock, C; Angel, R; Ciarlo, D; Fugate, R O; Ge, J; Kuzmenko, P; Lloyd-Hart, M; Macintosh, B; Najita, J; Woolf, N

    1999-07-27

    High resolution spectroscopy experiments with visible adaptive optics (AO) telescopes at Starfire Optical Range and Mt. Wilson have demonstrated that spectral resolution can be routinely improved by a factor of - 10 over the seeing-limited case with no extra light losses at visible wavelengths. With large CCDs now available, a very wide wavelength range can be covered in a single exposure. In the near future, most large ground-based telescopes will be equipped with powerful A0 systems. Most of these systems are aimed primarily at diffraction-limited operation in the near IR. An exciting new opportunity will thus open up for high resolution IR spectroscopy. Immersion echelle gratings with much coarser grooves being developed by us at LLNL will play a critical role in achieving high spectral resolution with a compact and low cost IR cryogenically cooled spectrograph and simultaneous large wavelength coverage on relatively small IR detectors. We have constructed a new A0 optimized spectrograph at Steward Observatory to provide R = 200,000 in the optical, which is being commissioned at the Starfire Optical Range 3.5m telescope. We have completed the optical design of the LLNL IR Immersion Spectrograph (LISPEC) to take advantage of improved silicon etching technology. Key words: adaptive optics, spectroscopy, high resolution, immersion gratings

  15. High Resolution Imagery of Haarp-Induced Optical Emissions

    NASA Astrophysics Data System (ADS)

    Kendall, E. A.

    2012-12-01

    One powerful technique for diagnosing radio frequency interactions in the ionosphere is to use ground-based optical instrumentation. High-frequency (HF), heater-induced optical emission observations can be used to diagnose electron energies and distributions in the heated region, illuminate natural and/or artificially induced ionospheric irregularities, determine ExB plasma drifts, and measure quenching rates by neutral species. Optical emissions are caused by HF-accelerated electrons colliding with various atmospheric constituents, which in turn emit a photon. The most common emissions are 630.0 nm O(1D), 557.7 nm O(1S), and 427.8 nm N2+(1NG). Since fairly wide field-of-view imagers are typically deployed in airglow campaigns, it is not well-known what meter-scale features exist in the artificial airglow emissions. Telescopic imaging provides high resolution spatial coverage of ionospheric irregularities and goes hand in hand with other observing techniques such as GPS scintillation, radar, and ionosonde. Imaging can be used to verify the interpretation of data from these other instruments, and this in turn allows confidence in such measurements when airglow cannot be observed (high solar angle or cloud cover). Telescopic imaging of airglow is the only technique capable of simultaneously determining the properties of ionospheric irregularities at decameter resolution over a range of several kilometers. The HAARP telescopic imager consists of two cameras, a set of optics for each camera, and a robotic mount that supports and orients the system. The camera and optics systems are identical except for the camera lenses: one has a wide-angle lens (~19 degrees) and the other has a telescopic lens (~3 degrees). The telescopic imager has a resolution of ~20 m in the F layer and ~10 m in the E layer, which allows the observation of decameter- and kilometer-scale features. Telescopic data has been recorded at HAARP for several years and images will be presented showing

  16. High-resolution adaptive optics findings in talc retinopathy.

    PubMed

    Soliman, Mohamed K; Sarwar, Salman; Hanout, Mostafa; Sadiq, Mohammad A; Agarwal, Aniruddha; Gulati, Vikas; Nguyen, Quan Dong; Sepah, Yasir J

    2015-01-01

    Talc retinopathy is a recognized ocular condition characterized by the presence of small, yellow, glistening crystals found inside small retinal vessels and within different retinal layers. These crystals can be associated with retinal vascular occlusion and ischemia. Different diagnostic modalities have been used previously to characterize the retinal lesions in talc retinopathy. Adaptive optics, a high resolution imaging technique, is used to evaluate the location, appearance and distribution of talc crystals in a case of talc retinopathy.

  17. Adaptive optics with pupil tracking for high resolution retinal imaging.

    PubMed

    Sahin, Betul; Lamory, Barbara; Levecq, Xavier; Harms, Fabrice; Dainty, Chris

    2012-02-01

    Adaptive optics, when integrated into retinal imaging systems, compensates for rapidly changing ocular aberrations in real time and results in improved high resolution images that reveal the photoreceptor mosaic. Imaging the retina at high resolution has numerous potential medical applications, and yet for the development of commercial products that can be used in the clinic, the complexity and high cost of the present research systems have to be addressed. We present a new method to control the deformable mirror in real time based on pupil tracking measurements which uses the default camera for the alignment of the eye in the retinal imaging system and requires no extra cost or hardware. We also present the first experiments done with a compact adaptive optics flood illumination fundus camera where it was possible to compensate for the higher order aberrations of a moving model eye and in vivo in real time based on pupil tracking measurements, without the real time contribution of a wavefront sensor. As an outcome of this research, we showed that pupil tracking can be effectively used as a low cost and practical adaptive optics tool for high resolution retinal imaging because eye movements constitute an important part of the ocular wavefront dynamics.

  18. Adaptive optics with pupil tracking for high resolution retinal imaging

    PubMed Central

    Sahin, Betul; Lamory, Barbara; Levecq, Xavier; Harms, Fabrice; Dainty, Chris

    2012-01-01

    Adaptive optics, when integrated into retinal imaging systems, compensates for rapidly changing ocular aberrations in real time and results in improved high resolution images that reveal the photoreceptor mosaic. Imaging the retina at high resolution has numerous potential medical applications, and yet for the development of commercial products that can be used in the clinic, the complexity and high cost of the present research systems have to be addressed. We present a new method to control the deformable mirror in real time based on pupil tracking measurements which uses the default camera for the alignment of the eye in the retinal imaging system and requires no extra cost or hardware. We also present the first experiments done with a compact adaptive optics flood illumination fundus camera where it was possible to compensate for the higher order aberrations of a moving model eye and in vivo in real time based on pupil tracking measurements, without the real time contribution of a wavefront sensor. As an outcome of this research, we showed that pupil tracking can be effectively used as a low cost and practical adaptive optics tool for high resolution retinal imaging because eye movements constitute an important part of the ocular wavefront dynamics. PMID:22312577

  19. Coralline red algae as high-resolution climate recorders

    NASA Astrophysics Data System (ADS)

    Halfar, J.; Steneck, R. S.; Joachimski, M.; Kronz, A.; Wanamaker, A. D., Jr.

    2008-06-01

    Most high-resolution, proxy-based paleoclimate research hasconcentrated on tropical oceans, while mid- and high-latitudemarine regions have received less attention, despite their importancein the global climate system. At present, sclerochronologicalanalyses of bivalve mollusks supply the bulk of annual- to subannual-resolutionextratropical marine climate data, even though interpretationis complicated by a slowdown of growth with increasing shellage. Hence, in order to address the need for additional high-resolutionproxy climate data from extratropical regions, we conductedthe first year-long in situ field calibration of the corallinered alga Clathromorphum compactum in the Gulf of Maine, UnitedStates. Coralline red algae are widely distributed in coastalregions worldwide, and individual calcified plants can livecontinuously for several centuries in temperate and subarcticoceans. Stable oxygen isotopes extracted at subannual resolutionfrom growth increments of monitored specimens of C. compactumrelate well to in situ-measured sea-surface temperaturesduring the May to December calcification period, highlightingthe suitability of coralline red algae as an extratropical climatearchive. Furthermore, there is a strong correlation betweena 30 yr {sigma}18O record of C. compactum and an instrumental sea-surfacetemperature record (r = -0.58, p = 0.0008) and a proxyreconstruction derived from the bivalve Arctica islandica collectedin the central Gulf of Maine (r = 0.54, p = 0.002).

  20. A high-resolution record of Greenland mass balance

    NASA Astrophysics Data System (ADS)

    McMillan, Malcolm; Leeson, Amber; Shepherd, Andrew; Briggs, Kate; Armitage, Thomas W. K.; Hogg, Anna; Kuipers Munneke, Peter; Broeke, Michiel; Noël, Brice; Berg, Willem Jan; Ligtenberg, Stefan; Horwath, Martin; Groh, Andreas; Muir, Alan; Gilbert, Lin

    2016-07-01

    We map recent Greenland Ice Sheet elevation change at high spatial (5 km) and temporal (monthly) resolution using CryoSat-2 altimetry. After correcting for the impact of changing snowpack properties associated with unprecedented surface melting in 2012, we find good agreement (3 cm/yr bias) with airborne measurements. With the aid of regional climate and firn modeling, we compute high spatial and temporal resolution records of Greenland mass evolution, which correlate (R = 0.96) with monthly satellite gravimetry and reveal glacier dynamic imbalance. During 2011-2014, Greenland mass loss averaged 269 ± 51 Gt/yr. Atmospherically driven losses were widespread, with surface melt variability driving large fluctuations in the annual mass deficit. Terminus regions of five dynamically thinning glaciers, which constitute less than 1% of Greenland's area, contributed more than 12% of the net ice loss. This high-resolution record demonstrates that mass deficits extending over small spatial and temporal scales have made a relatively large contribution to recent ice sheet imbalance.

  1. High-resolution second harmonic optical coherence tomography

    NASA Astrophysics Data System (ADS)

    Jiang, Yi; Tomov, Ivan V.; Wang, Yimin; Chen, Zhongping

    2005-04-01

    A high-resolution Second Harmonic Optical Coherence Tomography (SH-OCT) system is demonstrated using a spectrum broadened femtosecond Ti:sapphire laser. An axial resolution of 4.2 μm at the second harmonic wave center wavelength of 400 nm has been achieved. Because the SH-OCT system uses the second harmonic generation signals that strongly depend on the orientation, polarization and local symmetry properties of chiral molecules, this technique provides unique contrast enhancement to conventional optical coherence tomography. The system is applied to image biological tissues like the rat-tail tendon. Images of highly organized collagen fibrils in the rat-tail tendon have been demonstrated.

  2. All-Optical Ultrasound Transducers for High Resolution Imaging

    NASA Astrophysics Data System (ADS)

    Sheaff, Clay Smith

    High frequency ultrasound (HFUS) has increasingly been used within the past few decades to provide high resolution (< 200 mum) imaging in medical applications such as endoluminal imaging, intravascular imaging, ophthalmology, and dermatology. The optical detection and generation of HFUS using thin films offers numerous advantages over traditional piezoelectric technology. Circumvention of an electronic interface with the device head is one of the most significant given the RF noise, crosstalk, and reduced capacitance that encumbers small-scale electronic transducers. Thin film Fabry-Perot interferometers - also known as etalons - are well suited for HFUS receivers on account of their high sensitivity, wide bandwidth, and ease of fabrication. In addition, thin films can be used to generate HFUS when irradiated with optical pulses - a method referred to as Thermoelastic Ultrasound Generation (TUG). By integrating a polyimide (PI) film for TUG into an etalon receiver, we have created for the first time an all-optical ultrasound transducer that is both thermally stable and capable of forming fully sampled 2-D imaging arrays of arbitrary configuration. Here we report (1) the design and fabrication of PI-etalon transducers; (2) an evaluation of their optical and acoustic performance parameters; (3) the ability to conduct high-resolution imaging with synthetic 2-D arrays of PI-etalon elements; and (4) work towards a fiber optic PI-etalon for in vivo use. Successful development of a fiber optic imager would provide a unique field-of-view thereby exposing an abundance of prospects for minimally-invasive analysis, diagnosis, and treatment of disease.

  3. Optical Histology: High-Resolution Visualization of Tissue Microvasculature

    NASA Astrophysics Data System (ADS)

    Moy, Austin Jing-Ming

    Mammalian tissue requires the delivery of nutrients, growth factors, and the exchange of oxygen and carbon dioxide gases to maintain normal function. These elements are delivered by the blood, which travels through the connected network of blood vessels, known as the vascular system. The vascular system consists of large feeder blood vessels (arteries and veins) that are connected to the small blood vessels (arterioles and venules), which in turn are connected to the capillaries that are directly connected to the tissue and facilitate gas exchange and nutrient delivery. These small blood vessels and capillaries make up an intricate but organized network of blood vessels that exist in all mammalian tissues known as the microvasculature and are very important in maintaining the health and proper function of mammalian tissue. Due to the importance of the microvasculature in tissue survival, disruption of the microvasculature typically leads to tissue dysfunction and tissue death. The most prevalent method to study the microvasculature is visualization. Immunohistochemistry (IHC) is the gold-standard method to visualize tissue microvasculature. IHC is very well-suited for highly detailed interrogation of the tissue microvasculature at the cellular level but is unwieldy and impractical for wide-field visualization of the tissue microvasculature. The objective my dissertation research was to develop a method to enable wide-field visualization of the microvasculature, while still retaining the high-resolution afforded by optical microscopy. My efforts led to the development of a technique dubbed "optical histology" that combines chemical and optical methods to enable high-resolution visualization of the microvasculature. The development of the technique first involved preliminary studies to quantify optical property changes in optically cleared tissues, followed by development and demonstration of the methodology. Using optical histology, I successfully obtained high

  4. 3D high resolution pure optical photoacoustic microscopy

    NASA Astrophysics Data System (ADS)

    Xie, Zhixing; Chen, Sung-Liang; Ling, Tao; Guo, L. Jay; Carson, Paul L.; Wang, Xueding

    2012-02-01

    The concept of pure optical photoacoustic microscopy(POPAM) was proposed based on optical rastering of a focused excitation beam and optically sensing the photoacoustic signal using a microring resonator fabricated by a nanoimprinting technique. After some refinedment of in the resonator structure and mold fabrication, an ultrahigh Q factor of 3.0×105 was achieved which provided high sensitivity with a noise equivalent detectable pressure(NEDP) value of 29Pa. This NEDP is much lower than the hundreds of Pascals achieved with existing optical resonant structures such as etalons, fiber gratings and dielectric multilayer interference filters available for acoustic measurement. The featured high sensitivity allowed the microring resonator to detect the weak photoacoustic signals from micro- or submicroscale objects. The inherent superbroad bandwidth of the optical microring resonator combined with an optically focused scanning beam provided POPAM of high resolution in the axial as well as both lateral directions while the axial resolution of conventional photoacoustic microscopy (PAM) suffers from the limited bandwidth of PZT detectors. Furthermore, the broadband microring resonator showed similar sensitivity to that of our most sensitive PZT detector. The current POPAM system provides a lateral resolution of 5μm and an axial resolution of 8μm, comparable to that achieved by optical microscopy while presenting the unique contrast of optical absorption and functional information complementing other optical modalities. The 3D structure of microvasculature, including capillary networks, and even individual red blood cells have been discerned successfully in the proof-of-concept experiments on mouse bladders ex vivo and mouse ears in vivo. The potential of approximately GHz bandwidth of the microring resonator also might allow much higher resolution than shown here in microscopy of optical absorption and acoustic propagation properties at depths in unfrozen tissue

  5. Precision glass molding of high-resolution diffractive optical elements

    NASA Astrophysics Data System (ADS)

    Prater, Karin; Dukwen, Julia; Scharf, Toralf; Herzig, Hans P.; Plöger, Sven; Hermerschmidt, Andreas

    2016-04-01

    The demand of high resolution diffractive optical elements (DOE) is growing. Smaller critical dimensions allow higher deflection angles and can fulfill more demanding requirements, which can only be met by using electron-beam lithography. Replication techniques are more economical, since the high cost of the master can be distributed among a larger number of replicas. The lack of a suitable mold material for precision glass molding has so far prevented an industrial use. Glassy Carbon (GC) offers a high mechanical strength and high thermal strength. No anti-adhesion coatings are required in molding processes. This is clearly an advantage for high resolution, high aspect ratio microstructures, where a coating with a thickness between 10 nm and 200 nm would cause a noticeable rounding of the features. Electron-beam lithography was used to fabricate GC molds with highest precision and feature sizes from 250 nm to 2 μm. The master stamps were used for precision glass molding of a low Tg glass L-BAL42 from OHARA. The profile of the replicated glass is compared to the mold with the help of SEM images. This allows discussion of the max. aspect-ratio and min. feature size. To characterize optical performances, beamsplitting elements are fabricated and their characteristics were investigated, which are in excellent agreement to theory.

  6. ASIC-enabled High Resolution Optical Time Domain Reflectometer

    NASA Astrophysics Data System (ADS)

    Skendzic, Sandra

    Fiber optics has become the preferred technology in communication systems because of what it has to offer: high data transmission rates, immunity to electromagnetic interference, and lightweight, flexible cables. An optical time domain reflectometer (OTDR) provides a convenient method of locating and diagnosing faults (e.g. break in a fiber) along a fiber that can obstruct crucial optical pathways. Both the ability to resolve the precise location of the fault and distinguish between two discrete, closely spaced faults are figures of merit. This thesis presents an implementation of a high resolution OTDR through the use of a compact and programmable ASIC (application specific integrated circuit). The integration of many essential OTDR functions on a single chip is advantageous over existing commercial instruments because it enables small, lightweight packaging, and offers low power and cost efficiency. Furthermore, its compactness presents the option of placing multiple ASICs in parallel, which can conceivably ease the characterization of densely populated fiber optic networks. The OTDR ASIC consists of a tunable clock, pattern generator, precise timer, electrical receiver, and signal sampling circuit. During OTDR operation, the chip generates narrow electrical pulse, which can then be converted to optical format when coupled with an external laser diode driver. The ASIC also works with an external photodetector to measure the timing and amplitude of optical reflections in a fiber. It has a 1 cm sampling resolution, which allows for a 2 cm spatial resolution. While this OTDR ASIC has been previously demonstrated for multimode fiber fault diagnostics, this thesis focuses on extending its functionality to single mode fiber. To validate this novel approach to OTDR, this thesis is divided into five chapters: (1) introduction, (2) implementation, (3), performance of ASIC-based OTDR, (4) exploration in optical pre-amplification with a semiconductor optical amplifier, and

  7. High-resolution optical telescope for ultraviolet /UV/ radiation field

    NASA Technical Reports Server (NTRS)

    Karayan, W. W.

    1979-01-01

    Design techniques are discussed for all-reflecting optics from first-order system considerations and applications currently utilized in the field of astronomical optics. The solution of the Dall-Karkham design problem is described, showing the advantage of inexpensive construction as compared with higher order surfaces. The design process reported here is a F/5 collecting system which quickly mates directly with the spectrometer; it is capable of achieving desired high resolution and sensitivity requirements. The theoretical limit of aberration tolerances is achieved with less than 1/8 of a wavelength at final focus (OPD). The design of spectrometer for ultra-violet (UV) radiation and its mechanism is included in this study.

  8. Next generation high resolution adaptive optics fundus imager

    NASA Astrophysics Data System (ADS)

    Fournier, P.; Erry, G. R. G.; Otten, L. J.; Larichev, A.; Irochnikov, N.

    2005-12-01

    The spatial resolution of retinal images is limited by the presence of static and time-varying aberrations present within the eye. An updated High Resolution Adaptive Optics Fundus Imager (HRAOFI) has been built based on the development from the first prototype unit. This entirely new unit was designed and fabricated to increase opto-mechanical integration and ease-of-use through a new user interface. Improved camera systems for the Shack-Hartmann sensor and for the scene image were implemented to enhance the image quality and the frequency of the Adaptive Optics (AO) control loop. An optimized illumination system that uses specific wavelength bands was applied to increase the specificity of the images. Sample images of clinical trials of retinas, taken with and without the system, are shown. Data on the performance of this system will be presented, demonstrating the ability to calculate near diffraction-limited images.

  9. High-Resolution Mammography Detector Employing Optical Switching Readout

    NASA Astrophysics Data System (ADS)

    Irisawa, Kaku; Kaneko, Yasuhisa; Yamane, Katsutoshi; Sendai, Tomonari; Hosoi, Yuichi

    Conceiving a new detector structure, FUJIFILM Corporation has successfully put its invention of an X-ray detector employing "Optical Switching" into practical use. Since Optical Switching Technology allows an electrode structure to be easily designed, both high resolution of pixel pitch and low electrical noise readout have been achieved, which have consequently realized the world's smallest pixel size of 50×50 μm2 from a Direct-conversion FPD system as well as high DQE. The digital mammography system equipped with this detector enables to acquire high definition images while maintaining granularity. Its outstanding feature is to be able to acquire high-precision images of microcalcifications which is an important index in breast examination.

  10. High-Resolution Optical Tweezers for Single-Molecule Manipulation

    PubMed Central

    Zhang, Xinming; Ma, Lu; Zhang, Yongli

    2013-01-01

    Forces hold everything together and determine its structure and dynamics. In particular, tiny forces of 1-100 piconewtons govern the structures and dynamics of biomacromolecules. These forces enable folding, assembly, conformational fluctuations, or directional movements of biomacromolecules over sub-nanometer to micron distances. Optical tweezers have become a revolutionary tool to probe the forces, structures, and dynamics associated with biomacromolecules at a single-molecule level with unprecedented resolution. In this review, we introduce the basic principles of optical tweezers and their latest applications in studies of protein folding and molecular motors. We describe the folding dynamics of two strong coiled coil proteins, the GCN4-derived protein pIL and the SNARE complex. Both complexes show multiple folding intermediates and pathways. ATP-dependent chromatin remodeling complexes translocate DNA to remodel chromatin structures. The detailed DNA translocation properties of such molecular motors have recently been characterized by optical tweezers, which are reviewed here. Finally, several future developments and applications of optical tweezers are discussed. These past and future applications demonstrate the unique advantages of high-resolution optical tweezers in quantitatively characterizing complex multi-scale dynamics of biomacromolecules. PMID:24058311

  11. High-resolution optical tweezers for single-molecule manipulation.

    PubMed

    Zhang, Xinming; Ma, Lu; Zhang, Yongli

    2013-09-01

    Forces hold everything together and determine its structure and dynamics. In particular, tiny forces of 1-100 piconewtons govern the structures and dynamics of biomacromolecules. These forces enable folding, assembly, conformational fluctuations, or directional movements of biomacromolecules over sub-nanometer to micron distances. Optical tweezers have become a revolutionary tool to probe the forces, structures, and dynamics associated with biomacromolecules at a single-molecule level with unprecedented resolution. In this review, we introduce the basic principles of optical tweezers and their latest applications in studies of protein folding and molecular motors. We describe the folding dynamics of two strong coiled coil proteins, the GCN4-derived protein pIL and the SNARE complex. Both complexes show multiple folding intermediates and pathways. ATP-dependent chromatin remodeling complexes translocate DNA to remodel chromatin structures. The detailed DNA translocation properties of such molecular motors have recently been characterized by optical tweezers, which are reviewed here. Finally, several future developments and applications of optical tweezers are discussed. These past and future applications demonstrate the unique advantages of high-resolution optical tweezers in quantitatively characterizing complex multi-scale dynamics of biomacromolecules.

  12. Gemini high-resolution optical spectrograph conceptual design

    NASA Astrophysics Data System (ADS)

    Szeto, Kei; McConnachie, Alan; Anthony, André; Bohlender, David; Crampton, David; Desaulniers, Pierre; Dunn, Jennifer; Hardy, Tim; Hill, Alexis; Monin, Dmitry; Pazder, John; Schwab, Christian; Spano, Paola; Starkenburg, Else; Thibault, Simon; Walker, Gordon; Venn, Kim; Zhang, Hu

    2012-09-01

    A multiplexed moderate resolution (R = 34,000) and a single object high resolution (R = 90,000) spectroscopic facility for the entire 340 - 950nm wavelength region has been designed for Gemini. The result is a high throughput, versatile instrument that will enable precision spectroscopy for decades to come. The extended wavelength coverage for these relatively high spectral resolutions is achieved by use of an Echelle grating with VPH cross-dispersers and for the R = 90,000 mode utilization of an image slicer. The design incorporates a fast, efficient, reliable system for acquiring targets over the7 arcmin field of Gemini. This paper outlines the science case development and requirements flow-down process that leads to the configuration of the HIA instrument and describes the overall GHOS conceptual design. In addition, this paper discusses design trades examined during the conceptual design study instrument group of the Herzberg Institute of Astrophysics has been commissioned by the Gemini Observatory as one of the three competing organizations to conduct a conceptual design study for a new Gemini High-Resolution Optical Spectrograph (GHOS). This paper outlines the science case development and requirements flow-down process that leads to the configuration of the HIA instrument and describes the overall GHOS conceptual design. In addition, this paper discusses design trades examined during the conceptual design study.

  13. High-resolution optical fiber heterodyne interferometer for measuring displacement

    NASA Astrophysics Data System (ADS)

    Zhao, Yang; Wang, Jia; Cao, Mang; Li, Dacheng

    1990-07-01

    Many Methods have been developed to .easure displace.ent with high accuracy, for exap1e, with a dual frequency laser interferometer (AC interferometer) and an classic interferoseter (DC interferoeter) which use a stabilized laser and fringe counter, and an AC interfero.eter has ore advantage over the DC one. An AC interfero.eter with a Zee.an laser can get a high resolution, in the order of nanoMeters, but its resolution extension liRited by nonlinear relation between phase and displace.ent which caused by the two-frequency coRponents in interferoaeter[1]. Because the fundaaental length scale of the interferometer is the wavelength of the light source in the air. The accuracy of an interferoeter is li.ited by the operating envireaent, teRperature, husidity, pressure, etc. because the aiRs of interferoseters expose in the air. A high resolution optical fiber heterodyne interfermeter is described in the paper.

  14. HIGH RESOLUTION OPTICAL AND NIR SPECTRA OF HBC 722

    SciTech Connect

    Lee, Jeong-Eun; Park, Sunkyung; Green, Joel D.; Cochran, William D.; Kang, Wonseok; Lee, Sang-Gak; Sung, Hyun-Il E-mail: sunkyung@khu.ac.kr E-mail: wdc@astro.as.utexas.edu E-mail: sanggak@kywa.or.kr

    2015-07-01

    We present the results of high resolution (R ≥ 30,000) optical and near-IR spectroscopic monitoring observations of HBC 722, a recent FU Orionis object that underwent an accretion burst in 2010. We observed HBC 722 in the optical/near-IR with the Bohyunsan Optical Echelle Spectrograph, Hobby–Eberly Telescope-HRS, and Immersion Grating Infrared Spectrograph, at various points in the outburst. We found atomic lines with strongly blueshifted absorption features or P Cygni profiles, both evidence of a wind driven by the accretion. Some lines show a broad double-peaked absorption feature, evidence of disk rotation. However, the wind-driven and disk-driven spectroscopic features are anti-correlated in time; the disk features became strong as the wind features disappeared. This anti-correlation might indicate that the rebuilding of the inner disk was interrupted by the wind pressure during the first 2 years. The half-width at half-depth of the double-peaked profiles decreases with wavelength, indicative of the Keplerian rotation; the optical spectra with the disk feature are fitted by a G5 template stellar spectrum convolved with a rotation velocity of 70 km s{sup −1} while the near-IR disk features are fitted by a K5 template stellar spectrum convolved with a rotation velocity of 50 km s{sup −1}. Therefore, the optical and near-IR spectra seem to trace the disk at 39 and 76 R{sub ⊙}, respectively. We fit a power-law temperature distribution in the disk, finding an index of 0.8, comparable to optically thick accretion disk models.

  15. High Resolution Optical and NIR Spectra of HBC 722

    NASA Astrophysics Data System (ADS)

    Lee, Jeong-Eun; Park, Sunkyung; Green, Joel D.; Cochran, William D.; Kang, Wonseok; Lee, Sang-Gak; Sung, Hyun-Il

    2015-07-01

    We present the results of high resolution (R ≥ 30,000) optical and near-IR spectroscopic monitoring observations of HBC 722, a recent FU Orionis object that underwent an accretion burst in 2010. We observed HBC 722 in the optical/near-IR with the Bohyunsan Optical Echelle Spectrograph, Hobby-Eberly Telescope-HRS, and Immersion Grating Infrared Spectrograph, at various points in the outburst. We found atomic lines with strongly blueshifted absorption features or P Cygni profiles, both evidence of a wind driven by the accretion. Some lines show a broad double-peaked absorption feature, evidence of disk rotation. However, the wind-driven and disk-driven spectroscopic features are anti-correlated in time; the disk features became strong as the wind features disappeared. This anti-correlation might indicate that the rebuilding of the inner disk was interrupted by the wind pressure during the first 2 years. The half-width at half-depth of the double-peaked profiles decreases with wavelength, indicative of the Keplerian rotation; the optical spectra with the disk feature are fitted by a G5 template stellar spectrum convolved with a rotation velocity of 70 km s-1 while the near-IR disk features are fitted by a K5 template stellar spectrum convolved with a rotation velocity of 50 km s-1. Therefore, the optical and near-IR spectra seem to trace the disk at 39 and 76 R⊙, respectively. We fit a power-law temperature distribution in the disk, finding an index of 0.8, comparable to optically thick accretion disk models. Based on observations obtained with the Hobby-Eberly Telescope, which is a joint project of the University of Texas at Austin, the Pennsylvania State University, Stanford University, Ludwig-Maximilians-Universität München, and Georg-August-Universität Göttingen.

  16. Helium-neon laser: thermal high-resolution recording.

    PubMed

    Carlson, C O; Stone, E; Bernstein, H L; Tomita, W K; Myers, W C

    1966-12-23

    Scan-line recording by means of a moving laser spot has been achieved on metallic and organic thin films Recording rates of the order of one million spots per second were obtained with a laser beam power of 38 milliwatts at the recording surface. Typical recorded line widths were of the order of 2 microns.

  17. A New, Adaptable, Optical High-Resolution 3-Axis Sensor.

    PubMed

    Buchhold, Niels; Baumgartner, Christian

    2017-01-27

    This article presents a new optical, multi-functional, high-resolution 3-axis sensor which serves to navigate and can, for example, replace standard joysticks in medical devices such as electric wheelchairs, surgical robots or medical diagnosis devices. A light source, e.g., a laser diode, is affixed to a movable axis and projects a random geometric shape on an image sensor (CMOS or CCD). The downstream microcontroller's software identifies the geometric shape's center, distortion and size, and then calculates x, y, and z coordinates, which can be processed in attached devices. Depending on the image sensor in use (e.g., 6.41 megapixels), the 3-axis sensor features a resolution of 1544 digits from right to left and 1038 digits up and down. Through interpolation, these values rise by a factor of 100. A unique feature is the exact reproducibility (deflection to coordinates) and its precise ability to return to its neutral position. Moreover, optical signal processing provides a high level of protection against electromagnetic and radio frequency interference. The sensor is adaptive and adjustable to fit a user's range of motion (stroke and force). This recommendation aims to optimize sensor systems such as joysticks in medical devices in terms of safety, ease of use, and adaptability.

  18. A New, Adaptable, Optical High-Resolution 3-Axis Sensor

    PubMed Central

    Buchhold, Niels; Baumgartner, Christian

    2017-01-01

    This article presents a new optical, multi-functional, high-resolution 3-axis sensor which serves to navigate and can, for example, replace standard joysticks in medical devices such as electric wheelchairs, surgical robots or medical diagnosis devices. A light source, e.g., a laser diode, is affixed to a movable axis and projects a random geometric shape on an image sensor (CMOS or CCD). The downstream microcontroller’s software identifies the geometric shape’s center, distortion and size, and then calculates x, y, and z coordinates, which can be processed in attached devices. Depending on the image sensor in use (e.g., 6.41 megapixels), the 3-axis sensor features a resolution of 1544 digits from right to left and 1038 digits up and down. Through interpolation, these values rise by a factor of 100. A unique feature is the exact reproducibility (deflection to coordinates) and its precise ability to return to its neutral position. Moreover, optical signal processing provides a high level of protection against electromagnetic and radio frequency interference. The sensor is adaptive and adjustable to fit a user’s range of motion (stroke and force). This recommendation aims to optimize sensor systems such as joysticks in medical devices in terms of safety, ease of use, and adaptability. PMID:28134824

  19. High-resolution retinal imaging with micro adaptive optics system.

    PubMed

    Niu, Saisai; Shen, Jianxin; Liang, Chun; Zhang, Yunhai; Li, Bangming

    2011-08-01

    Based on the dynamic characteristics of human eye aberration, a microadaptive optics retina imaging system set is established for real-time wavefront measurement and correction. This paper analyzes the working principles of a 127-unit Hartmann-Shack wavefront sensor and a 37-channel micromachine membrane deformable mirror adopted in the system. The proposed system achieves wavefront reconstruction through the adaptive centroid detection method and the mode reconstruction algorithm of Zernike polynomials, so that human eye aberration can be measured accurately. Meanwhile, according to the adaptive optics aberration correction control model, a closed-loop iterative aberration correction algorithm based on Smith control is presented to realize efficient and real-time correction of human eye aberration with different characteristics, and characteristics of the time domain of the system are also optimized. According to the experiment results tested on a USAF 1951 standard resolution target and a living human retina (subject ZHY), the resolution of the system can reach 3.6 LP/mm, and the human eye wavefront aberration of 0.728λ (λ=785 nm) can be corrected to 0.081λ in root mean square (RMS) so as to achieve the diffraction limit (Strehl ratio is 0.866), then high-resolution retina images are obtained.

  20. Urban Ecosystems from Spaceborne High-Resolution Optical Data

    NASA Astrophysics Data System (ADS)

    Haas, Jan; Jacob, Alexander; Ban, Yifang

    2014-11-01

    The potential of high-resolution optical satellite images for mapping of ecologically important urban space is investigated in this study. Both a GeoEye-1 and a Landsat 8 scene over central Shanghai were first segmented by two different algorithms and then classified into seven urban classes by SVM. Shadows in the pan-sharpened GeoEye-1 image were masked out and replaced by the corresponding pan-sharpened classified Landsat 8 image. Largest confusions occurred between sealed and permeable but non-vegetated surfaces, and between low-rise residential and high-rise commercial buildings. Based on the classification result, ecosystem service balances, supply and demand was modelled for each particular land cover class. Classification accuracies of 88% and 91% could be reached, indicating the suitability of the underlying data and method for this application domain. The KTH-SEG segmentation algorithm slightly outperformed the one implemented in eCognition. The highest supply of ecosystem services was found in water bodies whereas high-rise built-up areas revealed largest demands.

  1. Urban Ecosystems from Spaceborne High-Resolution Optical Data

    NASA Astrophysics Data System (ADS)

    Haas, Jan; Jacob, Alexander; Ban, Yifang

    2014-11-01

    The potential of high-resolution optical satellite images for mapping of ecologically important urban space is investigated in this study. Both a GeoEye-1 and a Landsat 8 scene over central Shanghai were first segmented by two different algorithms and then classified into seven urban classes by SVM. Shadows in the pan-sharpened GeoEye-1 image were masked out and replaced by the corresponding pan-sharpened classified Landsat 8 image. Largest confusions occurred between sealed and permeable but non- vegetated surfaces, and between low-rise residential and high-rise commercial buildings. Based on the classification result, ecosystem service balances, supply and demand was modelled for each particular land cover class. Classification accuracies of 88% and 91% could be reached, indicating the suitability of the underlying data and method for this application domain. The KTH-SEG segmentation algorithm slightly outperformed the one implemented in eCognition. The highest supply of ecosystem services was found in water bodies whereas high-rise built-up areas revealed largest demands.

  2. Large Field, High Resolution Full-Field Optical Coherence Tomography

    PubMed Central

    Assayag, Osnath; Antoine, Martine; Sigal-Zafrani, Brigitte; Riben, Michael; Harms, Fabrice; Burcheri, Adriano; Grieve, Kate; Dalimier, Eugénie; Le Conte de Poly, Bertrand; Boccara, Claude

    2014-01-01

    We present a benchmark pilot study in which high-resolution Full-Field Optical Coherence Tomography (FF-OCT) was used to image human breast tissue and is evaluated to assess its ability to aid the pathologist’s management of intra-operative diagnoses. FF-OCT imaging safety was investigated and agreement between FF-OCT and routinely prepared histopathological images was evaluated. The compact setup used for this study provides 1 µm3 resolution and 200 µm imaging depth, and a 2.25 cm2 specimen is scanned in about 7 minutes. 75 breast specimens were imaged from 22 patients (21 women, 1 man) with a mean age of 58 (range: 25-83). Pathologists blind diagnosed normal/benign or malignant tissue based on FF-OCT images alone, diagnosis from histopathology followed for comparison. The contrast in the FF-OCT images is generated by intrinsic tissue scattering properties, meaning that no tissue staining or preparation is required. Major architectural features and tissue structures of benign breast tissue, including adipocytes, fibrous stroma, lobules and ducts were characterized. Subsequently, features resulting from pathological modification were characterized and a diagnosis decision tree was developed. Using FF-OCT images, two breast pathologists were able to distinguish normal/benign tissue from lesional with a sensitivity of 94% and 90%, and specificity of 75% and 79% respectively. PMID:24000981

  3. PEPSI, the High-Resolution Optical-IR Spectrograph for the LBT

    NASA Astrophysics Data System (ADS)

    Andersen, Michael; Strassmeier, Klaus; Hoffman, Axel; Woche, Manfred; Spano, Paolo

    PEPSI is a high resolution fibre feed optical-IR polarimetric echelle spectrograph for the Large Binocular Telescope (LBT). PEPSI utilizes the two 8.4m LBT apertures to simultaneously record four polarization states at a resolution of 120.000. The extension of the coverage towards the IR is mainly motivated by the larger Zeeman splitting of IR lines, which would allow to study weaker/fainter magnetic structures on stars. The two optical arms, which also have an integral light mode with R up to 300.000, are under construction, while the IR arm is being designed.

  4. Optical side scattering radiometry for high resolution, wide dynamic range longitudinal assessment of optical fibers.

    PubMed

    Sandoghchi, S R; Petrovich, M; Gray, D R; Chen, Y; Wheeler, N V; Bradley, T D; Wong, N H L; Jasion, G T; Hayes, J; Fokoua, E Numkam; Alonso, M B; Mousavi, S M; Richardson, D J; Poletti, F

    2015-10-19

    Current optical reflectometric techniques used to characterize optical fibers have to trade-off longitudinal range with spatial resolution and therefore struggle to provide simultaneously wide dynamic range (>20dB) and high resolution (<10cm). In this work, we develop and present a technique we refer to as Optical Side Scattering Radiometry (OSSR) capable of resolving discrete and distributed scattering properties of fibers along their length with up to 60dB dynamic range and 5cm spatial resolution. Our setup is first validated on a standard single mode telecoms fiber. Then we apply it to a record-length 11km hollow core photonic band-gap fiber (HC-PBGF) the characterization requirements of which lie far beyond the capability of standard optical reflectometric instruments. We next demonstrate use of the technique to investigate and explain the unusually high loss observed in another HC-PBGF and finally demonstrate its flexibility by measuring a HC-PBGF operating at a wavelength of 2µm. In all of these examples, good agreement between the OSSR measurements and other well-established (but more limited) characterization methods, i.e. cutback loss and OTDR, was obtained.

  5. Improved automatic optic nerve radius estimation from high resolution MRI

    NASA Astrophysics Data System (ADS)

    Harrigan, Robert L.; Smith, Alex K.; Mawn, Louise A.; Smith, Seth A.; Landman, Bennett A.

    2017-02-01

    The optic nerve (ON) is a vital structure in the human visual system and transports all visual information from the retina to the cortex for higher order processing. Due to the lack of redundancy in the visual pathway, measures of ON damage have been shown to correlate well with visual deficits. These measures are typically taken at an arbitrary anatomically defined point along the nerve and do not characterize changes along the length of the ON. We propose a fully automated, three-dimensionally consistent technique building upon a previous independent slice-wise technique to estimate the radius of the ON and surrounding cerebrospinal fluid (CSF) on high-resolution heavily T2-weighted isotropic MRI. We show that by constraining results to be three-dimensionally consistent this technique produces more anatomically viable results. We compare this technique with the previously published slice-wise technique using a short-term reproducibility data set, 10 subjects, follow-up <1 month, and show that the new method is more reproducible in the center of the ON. The center of the ON contains the most accurate imaging because it lacks confounders such as motion and frontal lobe interference. Long-term reproducibility, 5 subjects, follow-up of approximately 11 months, is also investigated with this new technique and shown to be similar to short-term reproducibility, indicating that the ON does not change substantially within 11 months. The increased accuracy of this new technique provides increased power when searching for anatomical changes in ON size amongst patient populations.

  6. Special issue on high-resolution optical imaging

    NASA Astrophysics Data System (ADS)

    Smith, Peter J. S.; Davis, Ilan; Galbraith, Catherine G.; Stemmer, Andreas

    2013-09-01

    The pace of development in the field of advanced microscopy is truly breath-taking, and is leading to major breakthroughs in our understanding of molecular machines and cell function. This special issue of Journal of Optics draws attention to a number of interesting approaches, ranging from fluorescence and imaging of unlabelled cells, to computational methods, all of which are describing the ever increasing detail of the dynamic behaviour of molecules in the living cell. This is a field which traditionally, and currently, demonstrates a marvellous interplay between the disciplines of physics, chemistry and biology, where apparent boundaries to resolution dissolve and living cells are viewed in ever more clarity. It is fertile ground for those interested in optics and non-conventional imaging to contribute high-impact outputs in the fields of cell biology and biomedicine. The series of articles presented here has been selected to demonstrate this interdisciplinarity and to encourage all those with a background in the physical sciences to 'dip their toes' into the exciting and dynamic discoveries surrounding cell function. Although single molecule super-resolution microscopy is commercially available, specimen preparation and interpretation of single molecule data remain a major challenge for scientists wanting to adopt the techniques. The paper by Allen and Davidson [1] provides a much needed detailed introduction to the practical aspects of stochastic optical reconstruction microscopy, including sample preparation, image acquisition and image analysis, as well as a brief description of the different variants of single molecule localization microscopy. Since super-resolution microscopy is no longer restricted to three-dimensional imaging of fixed samples, the review by Fiolka [2] is a timely introduction to techniques that have been successfully applied to four-dimensional live cell super-resolution microscopy. The combination of multiple high-resolution techniques

  7. High-resolution optical spectrum characterization using optical channel estimation and spectrum stitching technique.

    PubMed

    Jin, Chao; Bao, Yuan; Li, Zhaohui; Gui, Tao; Shang, Haiyan; Feng, Xinhuan; Li, Jianping; Yi, Xingwen; Yu, Changyuan; Li, Guifang; Lu, Chao

    2013-07-01

    A technique is proposed to measure the high-resolution and wide-band characterization of amplitude, phase responses, and polarization property of optical components. This technique combines the optical spectrum stitching and optical channel estimation methods. Two kinds of fiber Bragg grating based Fabry-Perot cavities with ultrafine structures have been characterized based on this technique. By using 1024 point fast Fourier transform and a narrow linewidth, wavelength-tunable laser source, a frequency resolution of ~10 MHz is realized with an optical measurement range beyond 250 GHz.

  8. High-resolution second-harmonic optical coherence tomography of collagen in rat-tail tendon

    NASA Astrophysics Data System (ADS)

    Jiang, Yi; Tomov, Ivan V.; Wang, Yimin; Chen, Zhongping

    2005-03-01

    A high-resolution second-harmonic optical coherence tomography (SH-OCT) system is demonstrated using a spectrum broadened femtosecond Ti :sapphire laser. An axial resolution of 4.2μm at the second-harmonic wave center wavelength of 400 nm has been achieved. Because the SH-OCT system uses the second-harmonic generation signals that strongly depend on the orientation, polarization, and local symmetry properties of chiral molecules, this technique provides unique contrast enhancement to conventional optical coherence tomography. The system is applied to image biological tissues of the rat-tail tendon. Highly organized collagen fibrils in the rat-tail tendon can be visualized in recorded images.

  9. Adaptive Optics Technology for High-Resolution Retinal Imaging

    PubMed Central

    Lombardo, Marco; Serrao, Sebastiano; Devaney, Nicholas; Parravano, Mariacristina; Lombardo, Giuseppe

    2013-01-01

    Adaptive optics (AO) is a technology used to improve the performance of optical systems by reducing the effects of optical aberrations. The direct visualization of the photoreceptor cells, capillaries and nerve fiber bundles represents the major benefit of adding AO to retinal imaging. Adaptive optics is opening a new frontier for clinical research in ophthalmology, providing new information on the early pathological changes of the retinal microstructures in various retinal diseases. We have reviewed AO technology for retinal imaging, providing information on the core components of an AO retinal camera. The most commonly used wavefront sensing and correcting elements are discussed. Furthermore, we discuss current applications of AO imaging to a population of healthy adults and to the most frequent causes of blindness, including diabetic retinopathy, age-related macular degeneration and glaucoma. We conclude our work with a discussion on future clinical prospects for AO retinal imaging. PMID:23271600

  10. Adaptive optics technology for high-resolution retinal imaging.

    PubMed

    Lombardo, Marco; Serrao, Sebastiano; Devaney, Nicholas; Parravano, Mariacristina; Lombardo, Giuseppe

    2012-12-27

    Adaptive optics (AO) is a technology used to improve the performance of optical systems by reducing the effects of optical aberrations. The direct visualization of the photoreceptor cells, capillaries and nerve fiber bundles represents the major benefit of adding AO to retinal imaging. Adaptive optics is opening a new frontier for clinical research in ophthalmology, providing new information on the early pathological changes of the retinal microstructures in various retinal diseases. We have reviewed AO technology for retinal imaging, providing information on the core components of an AO retinal camera. The most commonly used wavefront sensing and correcting elements are discussed. Furthermore, we discuss current applications of AO imaging to a population of healthy adults and to the most frequent causes of blindness, including diabetic retinopathy, age-related macular degeneration and glaucoma. We conclude our work with a discussion on future clinical prospects for AO retinal imaging.

  11. Bendable X-ray Optics for High Resolution Imaging

    NASA Technical Reports Server (NTRS)

    Gubarev, M.; Ramsey, B.; Kilaru, K.; Atkins, C.; Broadway, D.

    2014-01-01

    Current state-of the-art for x-ray optics fabrication calls for either the polishing of massive substrates into high-angular-resolution mirrors or the replication of thin, lower-resolution, mirrors from perfectly figured mandrels. Future X-ray Missions will require a change in this optics fabrication paradigm in order to achieve sub-arcsecond resolution in light-weight optics. One possible approach to this is to start with perfectly flat, light-weight surface, bend it into a perfect cone, form the desired mirror figure by material deposition, and insert the resulting mirror into a telescope structure. Such an approach is currently being investigated at MSFC, and a status report will be presented detailing the results of finite element analyses, bending tests and differential deposition experiments.

  12. Very high-resolution spectroscopy: the ESPRESSO optical design

    NASA Astrophysics Data System (ADS)

    Spanò, P.; Delabre, B.; Dekker, H.; Pepe, F.; Zerbi, F. M.; Di Marcantonio, P.; Cristiani, S.; Mégevand, D.

    2012-09-01

    Resolving power of spectrographs for large telescopes is generally limited by the maximum dimension of the dispersion gratings. To overcome this limit, innovative optical configurations have been designed, starting from the ideas proposed for CODEX. By properly combining pupil slicing and anamorphic magnification, a R~63'000-210'000 spectrograph has been designed. Many different solutions were proposed during the early design phases, and a detailed trade off study has been carried out to improve efficiency, manufacturability, and reduce risks and costs of the preliminary designs. We present a full description of the optical design of the spectrograph after preliminary design review, together with expected performances.

  13. High resolution measurement of water levels in optical components

    NASA Astrophysics Data System (ADS)

    Murrieta-Rico, Fabian N.; Petranovskii, Vitalii; Sergiyenko, Oleg; Hernandez-Balbuena, Daniel; Raymond-Herrera, Oscar

    2016-09-01

    Systems for optical analysis use vacuum chambers, where low pressures are reached. Remaining water molecules are the prevalent contaminant in high vacuum chambers. For this reason measurement of water levels is an important task that allows correct equipment operation. In this work, a different approach is presented for detecting and quantifying the water molecules inside a the vacuum chamber used in optical systems. A zeolite coated quartz crystal microbalance is used for detecting the water molecules, and the change in the resonance frequency is measured using a novel technique known as the principle of rational approximations. Theoretical results show how nanograms of adsorbed molecules are measured, and the number of molecules are quantified.

  14. On a possibility high resolution optical tomograph design

    NASA Astrophysics Data System (ADS)

    Khohlov, K. L.; Sokolov, V. K.

    2006-04-01

    An actual problem of the modern medicine is statement of the diagnosis of diseases of a thyroid gland of the person. Extreme sensitivity of this organ to a X-ray radiation and invasive to methods puts a problem about development of an optical tomograph of a high solution. For this purpose the method of coherent optical detection (CDT) in a combination to a posteriori handling can be used with the purpose of elimination of influence of a spread function of a figuring system.

  15. Optical system for high resolution spectrometer/monochromator

    DOEpatents

    Hettrick, Michael C.; Underwood, James H.

    1988-01-01

    An optical system for use in a spectrometer or monochromator employing a mirror which reflects electromagnetic radiation from a source to converge with same in a plane. A straight grooved, varied-spaced diffraction grating receives the converging electromagnetic radiation from the mirror and produces a spectral image for capture by a detector, target or like receiver.

  16. Optical system for high resolution spectrometer/monochromator

    DOEpatents

    Hettrick, M.C.; Underwood, J.H.

    1988-10-11

    An optical system for use in a spectrometer or monochromator employing a mirror which reflects electromagnetic radiation from a source to converge with same in a plane is disclosed. A straight grooved, varied-spaced diffraction grating receives the converging electromagnetic radiation from the mirror and produces a spectral image for capture by a detector, target or like receiver. 11 figs.

  17. High-resolution parallel optical coherence tomography in scattering samples

    NASA Astrophysics Data System (ADS)

    Laubscher, M.; Ducros, Mathieu G.; Karamata, Boris; Bourquin, Stephane; Lasser, Theo

    2001-11-01

    Parallel optical coherence tomography in scattering samples is demonstrated using a 58 by 58 smart-pixel detector array. A femtosecond mode-locked Ti:Sapphire laser in combination with a free space Michelson interferometer was employed to achieve 4micrometers longitudinal resolution and 9mm transverse resolution on a 260x260 micrometers 2 field of view. We imaged a resolution target covered by an intralipid solution with different scattering coefficients as well as onion cells.

  18. High-resolution proxy record of Holocene climate from a loess section in Southwestern Nebraska, USA

    USGS Publications Warehouse

    Miao, X.; Mason, J.A.; Johnson, W.C.; Wang, Hongfang

    2007-01-01

    Multi-proxy analysis was used to produce a high-resolution paleoclimatic record from an exceptionally thick section of the Holocene Bignell Loess near Wauneta, Southwestern Nebraska, in the central Great Plains. The Wauneta section has excellent age control, based on optically stimulated luminescence (OSL) and radiocarbon dating, and records multiple episodes of rapid loess deposition alternating with slower deposition and soil formation. The lowermost and uppermost OSL ages obtained from the Bignell Loess are 10,250 ?? 610??years (5.9??m depth) and 100 ?? 10??years (0.1??m depth), respectively. As a result, the Holocene has been temporally confined. Stratigraphically, the Bignell Loess overlies the Late Pleistocene Peoria Loess (deposited ??? 21-14??ka), and the two units are separated by the Brady Soil which is distinguished by its color and other pedogenic features. L*a*b* color parameters and organic carbon content of Bignell Loess are sensitive proxies to differentiate drought-induced aeolian sediment layers from the intercalated soil horizons. Soil organic carbon-derived ??13C data suggest that the C3-dominated floral environment during Peoria Loess deposition shifted dramatically to a C4-dominated environment during Brady Soil formation in response to a warming trend. Even greater C4 abundance characterized the late Holocene. High-resolution ??13C data support the contention that C3 vs. C4 vegetation change in the Holocene reflects ecosystem response to frequent vegetation disturbance under arid conditions. Time series analysis reveals that ??13C and color parameters display high frequency variation with periodicities of 103-118??years and 103??years, respectively. Similar periodicities were also reported in studies of North Dakota lakes, though the physical mechanism responsible is uncertain. Comparison of Bignell Loess color and tropical Pacific sea surface temperatures (SSTs) allows evaluation of a proposed teleconnection between drought in the Great

  19. High-resolution full-field spatial coherence gated optical tomography using monochromatic light source

    NASA Astrophysics Data System (ADS)

    Srivastava, Vishal; Nandy, Sreyankar; Singh Mehta, Dalip

    2013-09-01

    We demonstrate dispersion free, high-resolution full-field spatial coherence gated optical tomography using spatially incoherent monochromatic light source. Spatial coherence properties of light source were synthesized by means of combining a static diffuser and vibrating multi mode fiber bundle. Due to low spatial coherence of light source, the axial resolution of the system was achieved similar to that of conventional optical coherence tomography which utilizes low temporal coherence. Experimental results of fringe visibility versus optical path difference are presented for varying numerical apertures objective lenses. High resolution optically sectioned images of multilayer onion skin, and red blood cells are presented.

  20. High Resolution Aerosol Optical Thickness Retrievals for Air Quality Applications

    NASA Astrophysics Data System (ADS)

    Gupta, P.; Christopher, S. A.

    2006-05-01

    Due to its adverse effects on human health, global biota, climate and economy, the study of urban air quality and anthropogenic aerosols have gained significant attention of scientist, government and environmental agencies. Recent studies have shown the potential of monitoring particulate matter on a global basis primarily from polar orbiting satellites. Although the surface measurements of PM2.5 mass correlated well with satellite derived aerosol optical thickness (AOT) over selected locations, considerable challenges remains on using this relationship for regular monitoring and forecasting. The present retrieval of aerosol products from satellite measurements is more focused for climate change applications. For example the MODIS aerosol product is at 10X10 km2 grid cells. The goal of our study is to develop high spatial resolution aerosol optical thickness products over several AERONET and EPA PM2.5 monitoring stations in Eastern and South-East United States. Retrieval algorithm will make use of MODIS radiances observations and aerosols models for retrieval purpose will be derived from AERONET observations.

  1. Application of a continuously tunable, cw optical parametric oscillator for high-resolution spectroscopy.

    PubMed

    Gibson, G M; Dunn, M H; Padgett, M J

    1998-01-01

    We report the use of a smoothly tunable, single-frequency continuous-wave optical parametric oscillator (OPO) for high-resolution spectroscopy. The OPO is based on potassium titanyl phosphate and is resonant for both signal and idler fields, resulting in a device with a very low pump power threshold of 30 mW. The frequency-selective nature of the doubly resonant oscillator ensures that the signal and idler modes can be tuned across the entire phase-match bandwidth without the need for additional intracavity frequency-selective components. Smooth frequency tuning of the output of the OPO is obtained by tuning of the pump laser. To demonstrate the practicality of our OPO we recorded the absorption spectrum of cesium vapor in the 1-microm spectral region.

  2. High-resolution frequency domain second harmonic optical coherence tomography

    NASA Astrophysics Data System (ADS)

    Su, Jianping; Tomov, I. V.; Jiang, Yi; Chen, Zhongping

    2007-02-01

    We used continuum generated in an 8.5 cm long fiber by a femtosecond Yb fiber laser to improve threefold the axial resolution of frequency domain SH-OCT to 12μm. The acquisition time was shortened by more than two orders of magnitude compared to time domain SH-OCT. The system was applied to image biological tissue of fish scales, pig leg tendon and rabbit eye sclera. Highly organized collagen fibrils can be visualized in the recorded images. Polarization dependence on second harmonic has been used to obtain polarization resolved images.

  3. High-resolution fracture aperture mapping using optical profilometry

    NASA Astrophysics Data System (ADS)

    Ameli, Pasha; Elkhoury, Jean E.; Detwiler, Russell L.

    2013-10-01

    Fractures play an important role in the Earth's crust, often controlling both mechanical and transport processes. Developing a mechanistic understanding of these processes requires quantifying the roughness of fracture surfaces and the contacts and void spaces between fracture surfaces at high spatial resolution (10s of microns) over a broad range of scales (centimeters to meters). Here we present a scalable method for measuring fracture surfaces and reconstructing fracture aperture fields using an optical profilometer. We evaluate the method by measuring two fractured limestone cores; one is a tensile fracture with strong cross correlation between the surfaces and the other is a saw-cut, sand-blasted fracture with negligible cross correlation between the surfaces. Results of repeated measurements of these two fractures suggest that well-correlated surfaces, where the correlation between the surfaces can aid reconstruction, can be reproduced with local uncertainties with median standard deviation of 8 μm . Poorly correlated surfaces, where reconstruction relies solely upon the precision of the placement of the halves of the core on the profilometer stage, can be reproduced with local uncertainties with median standard deviation of 20 μm . Additionally, we quantified the accuracy of the technique by comparing calculated aperture profiles of a fractured concrete core to thin sections cut from the core after impregnating it with epoxy. The median deviation between the two measurements, which includes errors due to residual misalignment of the profiles, was 29 μm supporting the accuracy of the method. Our results emphasize the potential for using noncontact surface measurement techniques to accurately and precisely reconstruct fracture apertures over a wide range of length scales.

  4. High-Resolution Holocene Records of Paleoceanographic and Paleoclimatic Variability from the Southern Alaskan Continental Margin

    NASA Astrophysics Data System (ADS)

    Finney, B. P.; Jaeger, J. M.; Mix, A. C.; Cowan, E. A.; Gulick, S. S.; Mayer, L. A.; Pisias, N. G.; Powell, R. D.; Prahl, F.; Stoner, J. S.

    2004-12-01

    We are investigating sediments from the fjords and continental margin of southern Alaska to develop high-resolution climatic and oceanographic records for the Late Quaternary. Our goal is to better understand linkages between climatic, terrestrial and oceanic systems in this tectonically active and biologically productive region. A field program was conducted aboard the R/V Maurice Ewing in August/September 2004 utilizing geophysical surveys (high-resolution swath bathymetric and backscatter imaging, shallow sub-bottom profiling, and where permitted, high-resolution seismic reflection profiling), piston and multi-coring, and CTD/water sampling at about 30 sites in this region. Cores are being analyzed for sedimentological, microfossil, geochemical and stable isotopic proxies, with chronologies constrained by Pb-210, AMS radiocarbon, tephrochronolgic and paleomagnetic dating. Our preliminary results demonstrate that these rapidly accumulating sedimentary archives can resolve environmental changes on annual to decadal timescales. Records of recent changes in lithogenic sediment accumulation and biological productivity on the Gulf of Alaska shelf track historical climatic data that extends to the early 20th century in this region. The records also correlate with multi-decadal climate regimes during the Little Ice Age as suggested by tree-ring, glacial advance and salmon abundance records from nearby coastal sites. Jack Dymond's enthusiasm for collaborative, interdisciplinary research will help guide us in unraveling the fingerprints of key processes in this relatively unexplored region.

  5. High resolution hybrid optical and acoustic sea floor maps (Invited)

    NASA Astrophysics Data System (ADS)

    Roman, C.; Inglis, G.

    2013-12-01

    This abstract presents a method for creating hybrid optical and acoustic sea floor reconstructions at centimeter scale grid resolutions with robotic vehicles. Multibeam sonar and stereo vision are two common sensing modalities with complementary strengths that are well suited for data fusion. We have recently developed an automated two stage pipeline to create such maps. The steps can be broken down as navigation refinement and map construction. During navigation refinement a graph-based optimization algorithm is used to align 3D point clouds created with both the multibeam sonar and stereo cameras. The process combats the typical growth in navigation error that has a detrimental affect on map fidelity and typically introduces artifacts at small grid sizes. During this process we are able to automatically register local point clouds created by each sensor to themselves and to each other where they overlap in a survey pattern. The process also estimates the sensor offsets, such as heading, pitch and roll, that describe how each sensor is mounted to the vehicle. The end results of the navigation step is a refined vehicle trajectory that ensures the points clouds from each sensor are consistently aligned, and the individual sensor offsets. In the mapping step, grid cells in the map are selectively populated by choosing data points from each sensor in an automated manner. The selection process is designed to pick points that preserve the best characteristics of each sensor and honor some specific map quality criteria to reduce outliers and ghosting. In general, the algorithm selects dense 3D stereo points in areas of high texture and point density. In areas where the stereo vision is poor, such as in a scene with low contrast or texture, multibeam sonar points are inserted in the map. This process is automated and results in a hybrid map populated with data from both sensors. Additional cross modality checks are made to reject outliers in a robust manner. The final

  6. High resolution optical surface metrology with the slope measuring portable optical test system

    NASA Astrophysics Data System (ADS)

    Maldonado, Alejandro V.

    New optical designs strive to achieve extreme performance, and continually increase the complexity of prescribed optical shapes, which often require wide dynamic range and high resolution. SCOTS, or the Software Configurable Optical Test System, can measure a wide range of optical surfaces with high sensitivity using surface slope. This dissertation introduces a high resolution version of SCOTS called SPOTS, or the Slope measuring Portable Optical Test System. SPOTS improves the metrology of surface features on the order of sub-millimeter to decimeter spatial scales and nanometer to micrometer level height scales. Currently there is no optical surface metrology instrument with the same utility. SCOTS uses a computer controlled display (such as an LCD monitor) and camera to measure surface slopes over the entire surface of a mirror. SPOTS differs in that an additional lens is placed near the surface under test. A small prototype system is discussed in general, providing the support for the design of future SPOTS devices. Then the SCOTS instrument transfer function is addressed, which defines the way the system filters surface heights. Lastly, the calibration and performance of larger SPOTS device is analyzed with example measurements of the 8.4-m diameter aspheric Large Synoptic Survey Telescope's (LSST) primary mirror. In general optical systems have a transfer function, which filters data. In the case of optical imaging systems the instrument transfer function (ITF) follows the modulation transfer function (MTF), which causes a reduction of contrast as a function of increasing spatial frequency due to diffraction. In SCOTS, ITF is shown to decrease the measured height of surface features as their spatial frequency increases, and thus the SCOTS and SPOTS ITF is proportional to their camera system's MTF. Theory and simulations are supported by a SCOTS measurement of a test piece with a set of lithographically written sinusoidal surface topographies. In addition, an

  7. A high resolution magneto-optical system for imaging of individual magnetic flux quanta.

    PubMed

    Golubchik, Daniel; Polturak, Emil; Koren, Gad; Lipson, Stephen G

    2009-08-31

    A high-resolution magneto-optical imaging system is described. In this system magneto-optical Kerr effect is utilized for resolving individual flux quanta in a type II superconductor. Using an ultra thin EuSe indicator a spatial resolution of 0.8 microm is achieved.

  8. High-resolution handheld rigid endomicroscope based on full-field optical coherence tomography

    NASA Astrophysics Data System (ADS)

    Benoit a la Guillaume, Emilie; Martins, Franck; Boccara, Claude; Harms, Fabrice

    2016-02-01

    Full-field optical coherence tomography (FF-OCT) is a powerful tool for nondestructive assessment of biological tissue, i.e., for the structural examination of tissue in depth at a cellular resolution. Mostly known as a microscopy device for ex vivo analysis, FF-OCT has also been adapted to endoscopy setups since it shows good potential for in situ cancer diagnosis and biopsy guidance. Nevertheless, all the attempts to perform endoscopic FF-OCT imaging did not go beyond lab setups. We describe here, to the best of our knowledge, the first handheld FF-OCT endoscope based on a tandem interferometry assembly using incoherent illumination. A common-path passive imaging interferometer at the tip of an optical probe makes it robust and insensitive to environmental perturbations, and a low finesse Fabry-Perot processing interferometer guarantees a compact system. A good resolution (2.7 μm transverse and 6 μm axial) is maintained through the long distance, small diameter relay optics of the probe, and a good signal-to-noise ratio is achieved in a limited 100 ms acquisition time. High-resolution images and a movie of a rat brain slice have been recorded by moving the contact endoscope over the surface of the sample, allowing for tissue microscopic exploration at 20 μm under the surface. These promising ex vivo results open new perspectives for in vivo imaging of biological tissue, in particular, in the field of cancer and surgical margin assessment.

  9. Computational high-resolution optical imaging of the living human retina

    NASA Astrophysics Data System (ADS)

    Shemonski, Nathan D.; South, Fredrick A.; Liu, Yuan-Zhi; Adie, Steven G.; Scott Carney, P.; Boppart, Stephen A.

    2015-07-01

    High-resolution in vivo imaging is of great importance for the fields of biology and medicine. The introduction of hardware-based adaptive optics (HAO) has pushed the limits of optical imaging, enabling high-resolution near diffraction-limited imaging of previously unresolvable structures. In ophthalmology, when combined with optical coherence tomography, HAO has enabled a detailed three-dimensional visualization of photoreceptor distributions and individual nerve fibre bundles in the living human retina. However, the introduction of HAO hardware and supporting software adds considerable complexity and cost to an imaging system, limiting the number of researchers and medical professionals who could benefit from the technology. Here we demonstrate a fully automated computational approach that enables high-resolution in vivo ophthalmic imaging without the need for HAO. The results demonstrate that computational methods in coherent microscopy are applicable in highly dynamic living systems.

  10. High resolution 900 yr volcanic and climatic record from the Vostok area, East Antarctica

    NASA Astrophysics Data System (ADS)

    Osipov, E. Yu.; Khodzher, T. V.; Golobokova, L. P.; Onischuk, N. A.; Lipenkov, V. Ya.; Ekaykin, A. A.; Osipova, O. P.

    2013-05-01

    Detailed volcanic record of the last 900 yr (1093-2010 AD) has been received using high resolution (2-3 samples per accumulation year) sulfate measurements in four snow/firn cores from the Vostok station area, East Antarctica. Totally, 33 volcanic events have been identified in the record, including well-known low latitude eruption signals found in many polar ice cores (e.g., Pinatubo 1991, Agung 1963, Krakatoa 1883, Tambora 1815, Huanaputina 1600, Kuwae 1452), however in comparison with other Antarctic sites the record has more events covering the last 900 yr. The strongest volcanic signals occurred during mid-13th, mid-15th and 18th centuries. The largest volcanic signal of Vostok (both in sulfate concentration and flux) is the 1452 AD Kuwae eruption. Average snow accumulation rate calculated for the period 1093-2010 AD is 21.3 ± 2.3 mm H2O. Accumulation record demonstrates a slight positive trend, however sharply increased accumulation rate during the periods from 1600 to 1815 AD (by 11% from long-term mean) and from 1963 to 2010 AD (by 15%) are typical features of the site. Na+ record shows strong decadal-scale variability probably connected with coupled changes in atmospheric transport patterns over Antarctica (meridional circulation change) and local glaciology. The obtained high resolution climatic records suggest a high sensitivity of the Vostok location to environmental changes in Southern Hemisphere.

  11. The Gemini High-Resolution Optical SpecTrograph (GHOST) bench spectrograph optical design

    NASA Astrophysics Data System (ADS)

    Pazder, John; Burley, Greg; Ireland, Michael J.; Robertson, Gordon; Sheinis, Andrew; Zhelem, Ross

    2016-08-01

    Gemini High-Resolution Optical SpecTrograph (GHOST) is a fiber-fed spectrograph being developed for the Gemini telescope. GHOST is a white pupil échelle spectrograph with high efficiency and a broad continuous wavelength coverage (363-1000nm) with R>50,000 in two-object mode and >75,000 in single-object mode. The design incorporates a novel zero-Petzval sum white pupil relay to eliminate grating aberrations at the cross-dispersers. Cameras are based on non-achromatic designs with tilted detectors to eliminate the need for exotic glasses. This paper outlines the optical design of the bench-mounted spectrograph and the predicted spectrograph resolution and efficiency for the spectrograph.

  12. Design techniques and analysis of high-resolution neural recording systems targeting epilepsy focus localization.

    PubMed

    Shoaran, Mahsa; Pollo, Claudio; Leblebici, Yusuf; Schmid, Alexandre

    2012-01-01

    The design of a high-density neural recording system targeting epilepsy monitoring is presented. Circuit challenges and techniques are discussed to optimize the amplifier topology and the included OTA. A new platform supporting active recording devices targeting wireless and high-resolution focus localization in epilepsy diagnosis is also proposed. The post-layout simulation results of an amplifier dedicated to this application are presented. The amplifier is designed in a UMC 0.18µm CMOS technology, has an NEF of 2.19 and occupies a silicon area of 0.038 mm(2), while consuming 5.8 µW from a 1.8-V supply.

  13. An integral design strategy combining optical system and image processing to obtain high resolution images

    NASA Astrophysics Data System (ADS)

    Wang, Jiaoyang; Wang, Lin; Yang, Ying; Gong, Rui; Shao, Xiaopeng; Liang, Chao; Xu, Jun

    2016-05-01

    In this paper, an integral design that combines optical system with image processing is introduced to obtain high resolution images, and the performance is evaluated and demonstrated. Traditional imaging methods often separate the two technical procedures of optical system design and imaging processing, resulting in the failures in efficient cooperation between the optical and digital elements. Therefore, an innovative approach is presented to combine the merit function during optical design together with the constraint conditions of image processing algorithms. Specifically, an optical imaging system with low resolution is designed to collect the image signals which are indispensable for imaging processing, while the ultimate goal is to obtain high resolution images from the final system. In order to optimize the global performance, the optimization function of ZEMAX software is utilized and the number of optimization cycles is controlled. Then Wiener filter algorithm is adopted to process the image simulation and mean squared error (MSE) is taken as evaluation criterion. The results show that, although the optical figures of merit for the optical imaging systems is not the best, it can provide image signals that are more suitable for image processing. In conclusion. The integral design of optical system and image processing can search out the overall optimal solution which is missed by the traditional design methods. Especially, when designing some complex optical system, this integral design strategy has obvious advantages to simplify structure and reduce cost, as well as to gain high resolution images simultaneously, which has a promising perspective of industrial application.

  14. Plasmonic particles of colloidal silver in high-resolution recording media

    NASA Astrophysics Data System (ADS)

    Andreeva, O. V.; Andreeva, N. V.; Kuzmina, T. B.

    2017-01-01

    The optical properties of colloidal silver particles formed photographically in high-resolution silver halide photographic materials have been considered. The conditions that allow one to obtain exposed and developed light-sensitive silver halide particles in the form of colloidal particles of metallic silver having the properties of localized plasmons have been described. The results of the studies of the developed silver particles in traditional photographic materials for image holography and in nanoporous silver halide photographic materials for volume holography have been presented. The perspectives of using plasmonic silver nanoparticles produced photographically have been discussed.

  15. Optic for an endoscope/borescope having high resolution and narrow field of view

    DOEpatents

    Stone, Gary F.; Trebes, James E.

    2003-10-28

    An optic having optimized high spatial resolution, minimal nonlinear magnification distortion while at the same time having a limited chromatic focal shift or chromatic aberrations. The optic located at the distal end of an endoscopic inspection tool permits a high resolution, narrow field of view image for medical diagnostic applications, compared to conventional optics for endoscopic instruments which provide a wide field of view, low resolution image. The image coverage is over a narrow (<20 degrees) field of view with very low optical distortion (<5% pin cushion or barrel distortion. The optic is also optimized for best color correction as well as to aid medical diagnostics.

  16. High resolution atomic coherent control via spectral phase manipulation of an optical frequency comb.

    PubMed

    Stowe, Matthew C; Cruz, Flavio C; Marian, Adela; Ye, Jun

    2006-04-21

    We demonstrate high resolution coherent control of cold atomic rubidium utilizing spectral phase manipulation of a femtosecond optical frequency comb. Transient coherent accumulation is directly manifested by the enhancement of signal amplitude and spectral resolution via the pulse number. The combination of frequency comb technology and spectral phase manipulation enables coherent control techniques to enter a new regime with natural linewidth resolution.

  17. High Resolution Atomic Coherent Control via Spectral Phase Manipulation of an Optical Frequency Comb

    SciTech Connect

    Stowe, Matthew C.; Cruz, Flavio C.; Marian, Adela; Ye Jun

    2006-04-21

    We demonstrate high resolution coherent control of cold atomic rubidium utilizing spectral phase manipulation of a femtosecond optical frequency comb. Transient coherent accumulation is directly manifested by the enhancement of signal amplitude and spectral resolution via the pulse number. The combination of frequency comb technology and spectral phase manipulation enables coherent control techniques to enter a new regime with natural linewidth resolution.

  18. High-Resolution Adaptive Optics Test-Bed for Vision Science

    SciTech Connect

    Wilks, S C; Thomspon, C A; Olivier, S S; Bauman, B J; Barnes, T; Werner, J S

    2001-09-27

    We discuss the design and implementation of a low-cost, high-resolution adaptive optics test-bed for vision research. It is well known that high-order aberrations in the human eye reduce optical resolution and limit visual acuity. However, the effects of aberration-free eyesight on vision are only now beginning to be studied using adaptive optics to sense and correct the aberrations in the eye. We are developing a high-resolution adaptive optics system for this purpose using a Hamamatsu Parallel Aligned Nematic Liquid Crystal Spatial Light Modulator. Phase-wrapping is used to extend the effective stroke of the device, and the wavefront sensing and wavefront correction are done at different wavelengths. Issues associated with these techniques will be discussed.

  19. On the optical stability of high-resolution transmission electron microscopes.

    PubMed

    Barthel, J; Thust, A

    2013-11-01

    In the recent two decades the technique of high-resolution transmission electron microscopy experienced an unprecedented progress through the introduction of hardware aberration correctors and by the improvement of the achievable resolution to the sub-Ångström level. The important aspect that aberration correction at a given resolution requires also a well defined amount of optical stability has received little attention so far. Therefore we investigate the qualification of a variety of high-resolution electron microscopes to maintain an aberration corrected optical state in terms of an optical lifetime. We develop a comprehensive statistical framework for the estimation of the optical lifetime and find remarkably low values between tens of seconds and a couple of minutes. Probability curves are introduced, which inform the operator about the chance to work still in the fully aberration corrected state.

  20. Polarization maintaining fiber based ultra-high resolution spectral domain polarization sensitive optical coherence tomography

    PubMed Central

    2010-01-01

    We present a new ultra high resolution spectral domain polarization sensitive optical coherence tomography (PS-OCT) system based on polarization maintaining (PM) fibers. The method transfers the principles of our previous bulk optic PS-OCT systems to a fiberized setup. The phase shift between the orthogonal polarization states travelling in the two orthogonal modes of the PM fiber is compensated by software in post processing. Thereby, the main advantage of our bulk optics setups, i.e. the use of only a single input polarization state to simultaneously acquire reflectivity, retardation, optic axis orientation, and Stokes vector, is maintained. The use of a broadband light source of 110 nm bandwidth provides improved depth resolution and smaller speckle size. The latter is important for improved resolution of depolarization imaging. We demonstrate our instrument for high-resolution PS-OCT imaging of the healthy human retina. PMID:20052196

  1. [High-resolution eye movement recording in the assessment of neurologic complications in HIV-1 infection].

    PubMed

    Currie, J; Ramsden, B; McArthur, C; Lunch, J; Maruff, P; Benson, E; Perdices, M; Cooper, D

    1989-12-01

    HIV-1 related brain disease gives rise to widespread eye movement abnormalities that include impairment of fixation, saccadic speed and accuracy, antisaccadic generation and smooth pursuit function. Quantitative high resolution recording of eye movements is a valuable, non-invasive technique both for measuring the severity and progression of the AIDS dementia complex and the early detection of neurologic dysfunction in asymptomatic HIV-seropositive subjects or in patients with AIDS. In particular, it may be of use in neurologically at-risk patients requiring antiviral therapy and in monitoring the neurologic responses to such treatment.

  2. High resolution 3D dosimetry for microbeam radiation therapy using optical CT

    NASA Astrophysics Data System (ADS)

    McErlean, C.; Bräuer-Krisch, E.; Adamovics, J.; Leach, M. O.; Doran, S. J.

    2015-01-01

    Optical Computed Tomography (CT) is a promising technique for dosimetry of Microbeam Radiation Therapy (MRT), providing high resolution 3D dose maps. Here different MRT irradiation geometries are visualised showing the potential of Optical CT as a tool for future MRT trials. The Peak-to-Valley dose ratio (PVDR) is calculated to be 7 at a depth of 3mm in the radiochromic dosimeter PRESAGE®. This is significantly lower than predicted values and possible reasons for this are discussed.

  3. High-resolution photometric optical monitoring for thin-film deposition.

    PubMed

    Rabady, Rabi; Zinoviev, Kirill; Avrutsky, Ivan

    2004-01-01

    Real-time monitoring of thin-film deposition with high resolution is important for precise fabrication of thin-film devices in a technological environment with ever-increasing demands for smaller size and better performance. Using photometry, we were able to achieve a real-time optical monitoring resolution of film thickness that is comparable with a single atomic layer scale (i.e., subnanometer). Filtering noise efficiently and compensating for sources of error by use of an appropriate model produced this high resolution. The procedure proved reliable and can be useful in the thin-film-deposition industry.

  4. Analysis and modeling of atmospheric turbulence on the high-resolution space optical systems

    NASA Astrophysics Data System (ADS)

    Lili, Jiang; Chen, Xiaomei; Ni, Guoqiang

    2016-09-01

    Modeling and simulation of optical remote sensing system plays an unslightable role in remote sensing mission predictions, imaging system design, image quality assessment. It has already become a hot research topic at home and abroad. Atmospheric turbulence influence on optical systems is attached more and more importance to as technologies of remote sensing are developed. In order to study the influence of atmospheric turbulence on earth observation system, the atmospheric structure parameter was calculated by using the weak atmospheric turbulence model; and the relationship of the atmospheric coherence length and high resolution remote sensing optical system was established; then the influence of atmospheric turbulence on the coefficient r0h of optical remote sensing system of ground resolution was derived; finally different orbit height of high resolution optical system imaging quality affected by atmospheric turbulence was analyzed. Results show that the influence of atmospheric turbulence on the high resolution remote sensing optical system, the resolution of which has reached sub meter level meter or even the 0.5m, 0.35m and even 0.15m ultra in recent years, image quality will be quite serious. In the above situation, the influence of the atmospheric turbulence must be corrected. Simulation algorithms of PSF are presented based on the above results. Experiment and analytical results are posted.

  5. Optical fibers for high-resolution in vivo microendoscopic fluorescence imaging

    NASA Astrophysics Data System (ADS)

    Oh, Gyungseok; Chung, Euiheon; Yun, Seok H.

    2013-12-01

    Optical fiber-based high-resolution fluorescence imaging techniques have promising applications in clinical practice and preclinical research using animals. Here we review the instrumentation and applications of microendoscopy based on various types of optical fibers. Single-mode fibers and double-clad fibers have been widely used for delivering light from light sources to tissues and collecting light from tissues to photodetectors. Coherent fiber bundles, cylindrical graded-index lenses, and multi-mode fibers have been employed in both beam-scanning and non-scanning microscopy. With continuing advances of optical fiber technologies, further innovations in optical microendoscopy are expected.

  6. Ultra-high aspect ratio high-resolution nanofabrication for hard X-ray diffractive optics.

    PubMed

    Chang, Chieh; Sakdinawat, Anne

    2014-06-27

    Although diffractive optics have played a major role in nanoscale soft X-ray imaging, high-resolution and high-efficiency diffractive optics have largely been unavailable for hard X-rays where many scientific, technological and biomedical applications exist. This is owing to the long-standing challenge of fabricating ultra-high aspect ratio high-resolution dense nanostructures. Here we report significant progress in ultra-high aspect ratio nanofabrication of high-resolution, dense silicon nanostructures using vertical directionality controlled metal-assisted chemical etching. The resulting structures have very smooth sidewalls and can be used to pattern arbitrary features, not limited to linear or circular. We focus on the application of X-ray zone plate fabrication for high-efficiency, high-resolution diffractive optics, and demonstrate the process with linear, circular, and spiral zone plates. X-ray measurements demonstrate high efficiency in the critical outer layers. This method has broad applications including patterning for thermoelectric materials, battery anodes and sensors among others.

  7. High-resolution imaging of biological tissue with full-field optical coherence tomography

    NASA Astrophysics Data System (ADS)

    Zhu, Yue; Gao, Wanrong

    2015-03-01

    A new full-field optical coherence tomography system with high-resolution has been developed for imaging of cells and tissues. Compared with other FF-OCT (Full-field optical coherence tomography, FF-OCT) systems illuminated with optical fiber bundle, the improved Köhler illumination arrangement with a halogen lamp was used in the proposed FF-OCT system. High numerical aperture microscopic objectives were used for imaging and a piezoelectric ceramic transducer (PZT) was used for phase-shifting. En-face tomographic images can be obtained by applying the five-step phase-shifting algorithm to a series of interferometric images which are recorded by a smart camera. Three-dimensional images can be generated from these tomographic images. Imaging of the chip of Intel Pentium 4 processor demonstrated the ultrahigh resolution of the system (lateral resolution is 0.8μm ), which approaches the theoretical resolution 0.7 μm× 0.5 μm (lateral × axial). En-face images of cells of onion show an excellent performance of the system in generating en-face images of biological tissues. Then, unstained pig stomach was imaged as a tissue and gastric pits could be easily recognized using FF-OCT system. Our study provides evidence for the potential ability of FFOCT in identifying gastric pits from pig stomach tissue. Finally, label-free and unstained ex vivo human liver tissues from both normal and tumor were imaged with this FFOCT system. The results show that the setup has the potential for medical diagnosis applications such liver cancer diagnosis.

  8. Optimal site selection for a high resolution ice core record in East Antarctica

    NASA Astrophysics Data System (ADS)

    Vance, T.; Roberts, J.; Moy, A.; Curran, M.; Tozer, C.; Gallant, A.; Abram, N.; van Ommen, T.; Young, D.; Grima, C.; Blankenship, D.; Siegert, M.

    2015-11-01

    Ice cores provide some of the best dated and most comprehensive proxy records, as they yield a vast and growing array of proxy indicators. Selecting a site for ice core drilling is nonetheless challenging, as the assessment of potential new sites needs to consider a variety of factors. Here, we demonstrate a systematic approach to site selection for a new East Antarctic high resolution ice core record. Specifically, seven criteria are considered: (1) 2000 year old ice at 300 m depth, (2) above 1000 m elevation, (3) a minimum accumulation rate of 250 mm yr-1 IE, (4) minimal surface re-working to preserve the deposited climate signal, (5) a site with minimal displacement or elevation change of ice at 300 m depth, (6) a strong teleconnection to mid-latitude climate and (7) an appropriately complementary relationship to the existing Law Dome record (a high resolution record in East Antarctica). Once assessment of these physical characteristics identified promising regions, logistical considerations (for site access and ice core retrieval) were briefly considered. We use Antarctic surface mass balance syntheses, along with ground-truthing of satellite data by airborne radar surveys to produce all-of-Antarctica maps of surface roughness, age at specified depth, elevation and displacement change and surface air temperature correlations to pinpoint promising locations. We also use the European Centre for Medium-Range Weather Forecast ERA 20th Century reanalysis (ERA-20C) to ensure a site complementary to the Law Dome record is selected. We find three promising sites in the Indian Ocean sector of East Antarctica in the coastal zone from Enderby Land to the Ingrid Christensen Coast (50-100° E). Although we focus on East Antarctica for a new ice core site, the methodology is more generally applicable and we include key parameters for all of Antarctica which may be useful for ice core site selection elsewhere and/or for other purposes.

  9. Optimal site selection for a high-resolution ice core record in East Antarctica

    NASA Astrophysics Data System (ADS)

    Vance, Tessa R.; Roberts, Jason L.; Moy, Andrew D.; Curran, Mark A. J.; Tozer, Carly R.; Gallant, Ailie J. E.; Abram, Nerilie J.; van Ommen, Tas D.; Young, Duncan A.; Grima, Cyril; Blankenship, Don D.; Siegert, Martin J.

    2016-03-01

    Ice cores provide some of the best-dated and most comprehensive proxy records, as they yield a vast and growing array of proxy indicators. Selecting a site for ice core drilling is nonetheless challenging, as the assessment of potential new sites needs to consider a variety of factors. Here, we demonstrate a systematic approach to site selection for a new East Antarctic high-resolution ice core record. Specifically, seven criteria are considered: (1) 2000-year-old ice at 300 m depth; (2) above 1000 m elevation; (3) a minimum accumulation rate of 250 mm years-1 IE (ice equivalent); (4) minimal surface reworking to preserve the deposited climate signal; (5) a site with minimal displacement or elevation change in ice at 300 m depth; (6) a strong teleconnection to midlatitude climate; and (7) an appropriately complementary relationship to the existing Law Dome record (a high-resolution record in East Antarctica). Once assessment of these physical characteristics identified promising regions, logistical considerations (for site access and ice core retrieval) were briefly considered. We use Antarctic surface mass balance syntheses, along with ground-truthing of satellite data by airborne radar surveys to produce all-of-Antarctica maps of surface roughness, age at specified depth, elevation and displacement change, and surface air temperature correlations to pinpoint promising locations. We also use the European Centre for Medium-Range Weather Forecast ERA 20th Century reanalysis (ERA-20C) to ensure that a site complementary to the Law Dome record is selected. We find three promising sites in the Indian Ocean sector of East Antarctica in the coastal zone from Enderby Land to the Ingrid Christensen Coast (50-100° E). Although we focus on East Antarctica for a new ice core site, the methodology is more generally applicable, and we include key parameters for all of Antarctica which may be useful for ice core site selection elsewhere and/or for other purposes.

  10. Bivariable analysis of ventricular late potentials in high resolution ECG records

    NASA Astrophysics Data System (ADS)

    Orosco, L.; Laciar, E.

    2007-11-01

    In this study the bivariable analysis for ventricular late potentials detection in high-resolution electrocardiographic records is proposed. The standard time-domain analysis and the application of the time-frequency technique to high-resolution ECG records are briefly described as well as their corresponding results. In the proposed technique the time-domain parameter, QRSD and the most significant time-frequency index, ENQRS are used like variables. A bivariable index is defined, that combines the previous parameters. The propose technique allows evaluating the risk of ventricular tachycardia in post-myocardial infarct patients. The results show that the used bivariable index allows discriminating between the patient's population with ventricular tachycardia and the subjects of the control group. Also, it was found that the bivariable technique obtains a good valuation as diagnostic test. It is concluded that comparatively, the valuation of the bivariable technique as diagnostic test is superior to that of the time-domain method and the time-frequency technique evaluated individually.

  11. A High-resolution Detrital and Oxygen Isotope Record from Flemish Pass, Labrador Sea

    NASA Astrophysics Data System (ADS)

    deJesus, E.; Hoffman, J. S.; Clark, P. U.; Mix, A. C.

    2014-12-01

    High-resolution records of deglacial paleoceanographic change along the Labrador shelf are scarce. However, they are required in order to characterize and understand possible ice-ocean interactions involving the eastern sector of the Laurentide Ice Sheet (LIS). We have generated a high-resolution stable isotope and detrital stratigraphic record for core HU2001043-008 (990m, 48° N, 45° W) from Flemish Pass, Labrador Sea, to better understand the role of LIS ice-rafting events in abrupt climate changes during the last glaciation. Samples at two-centimeter resolution were disaggregated, washed, and picked for Neogloboquandrina pachyderma (sinistral) for stable isotope and radiocarbon analysis. The δ18O signal in foraminiferal calcite allows us to examine surface-ocean changes that may indicate an influx of freshwater, which may or may not be related to an LIS ice-rafting event. Our results will help in developing a better understanding of the source of LIS ice-rafting events, precursory indicators of the events, and how these events are associated with changes in deep-water formation in the Labrador Sea.

  12. Fiber optic cable-based high-resolution, long-distance VGA extenders

    NASA Astrophysics Data System (ADS)

    Rhee, Jin-Geun; Lee, Iksoo; Kim, Heejoon; Kim, Sungjoon; Koh, Yeon-Wan; Kim, Hoik; Lim, Jiseok; Kim, Chur; Kim, Jungwon

    2013-02-01

    Remote transfer of high-resolution video information finds more applications in detached display applications for large facilities such as theaters, sports complex, airports, and security facilities. Active optical cables (AOCs) provide a promising approach for enhancing both the transmittable resolution and distance that standard copper-based cables cannot reach. In addition to the standard digital formats such as HDMI, the high-resolution, long-distance transfer of VGA format signals is important for applications where high-resolution analog video ports should be also supported, such as military/defense applications and high-resolution video camera links. In this presentation we present the development of a compressionless, high-resolution (up to WUXGA, 1920x1200), long-distance (up to 2 km) VGA extenders based on serialized technique. We employed asynchronous serial transmission and clock regeneration techniques, which enables lower cost implementation of VGA extenders by removing the necessity for clock transmission and large memory at the receiver. Two 3.125-Gbps transceivers are used in parallel to meet the required maximum video data rate of 6.25 Gbps. As the data are transmitted asynchronously, 24-bit pixel clock time stamp is employed to regenerate video pixel clock accurately at the receiver side. In parallel to the video information, stereo audio and RS-232 control signals are transmitted as well.

  13. High-Resolution Imaging of the Optic Nerve and Retina in Optic Nerve Hypoplasia

    PubMed Central

    Pilat, Anastasia; Sibley, Daniel; McLean, Rebecca J.; Proudlock, Frank A.; Gottlob, Irene

    2015-01-01

    Purpose To investigate the optic nerve and macular morphology in patients with optic nerve hypoplasia (ONH) using spectral-domain optical coherence tomography (SD OCT). Design Prospective, cross-sectional, observational study. Subjects A total of 16 participants with ONH (10 female and 6 male; mean age, 17.2 years; 6 bilateral involvement) and 32 gender-, age-, ethnicity-, and refraction-matched healthy controls. Methods High-resolution SD OCT (Copernicus [Optopol Technology S.A., Zawiercie, Poland], 3 μm resolution) and handheld SD OCT (Bioptigen Inc [Research Triangle Park, NC], 2.6 μm resolution) devices were used to acquire horizontal scans through the center of the optic disc and macula. Main Outcome Measures Horizontal optic disc/cup and rim diameters, cup depth, peripapillary retinal nerve fiber layer (RNFL), and thickness of individual retinal layers in participants with ONH and in controls. Results Patients with ONH had significantly smaller discs (P < 0.03 and P < 0.001 compared with unaffected eye and healthy controls, respectively), horizontal cup diameter (P < 0.02 for both), and cup depth (P < 0.02 and P < 0.01, respectively). In the macula, significantly thinner RNFL (nasally), ganglion cell layer (GCL) (nasally and temporally), inner plexiform layer (IPL) (nasally), outer nuclear layer (ONL) (nasally), and inner segment (centrally and temporally) were found in patients with ONH compared with the control group (P < 0.05 for all comparisons). Continuation of significantly thicker GCL, IPL, and outer plexiform layer in the central retinal area (i.e., foveal hypoplasia) was found in more than 80% of patients with ONH. Clinically unaffected fellow eyes of patients with ONH showed mild features of underdevelopment. Visual acuity and presence of septo-optic dysplasia were associated with changes in GCL and IPL. Sensitivity and specificity for the detection of ONH based on disc and retinal optical coherence tomography (OCT) parameters were >80

  14. High-resolution optical spectroscopy of RS Ophiuchi during 2008-2009

    NASA Astrophysics Data System (ADS)

    Somero, A.; Hakala, P.; Wynn, G. A.

    2017-01-01

    RS Ophiuchi (RS Oph) is a symbiotic variable and a recurrent nova (RN). We have monitored it with the Nordic Optical Telescope and obtained 30 high-resolution (R = 46 000) optical spectra over one orbital cycle during quiescence. To our knowledge, this is the best-sampled high-resolution spectroscopic data set of RS Oph over one orbital period. We do not detect any direct signatures of an accretion disc such as double peaked emission lines, but many line profiles are complex consisting of superimposed emission and absorption components. We measure the spin of the red giant and conclude that it is tidally locked to the binary orbit. We observe Na I absorption features, probably arising from the circumbinary medium, that has been shaped by previous RN outbursts. We do not detect any intrinsic polarization in the optical wavelengths.

  15. a Decade-Spanning High-Resolution Asynchronous Optical Sampling Based Terahertz Time-Domain Spectrometer

    NASA Astrophysics Data System (ADS)

    Good, Jacob T.; Holland, Daniel; Finneran, Ian A.; Carroll, Brandon; Allodi, Marco A.; Blake, Geoffrey

    2015-06-01

    High-resolution ASynchronous OPtical Sampling (ASOPS) is a technique that substantially improves the combined frequency resolution and bandwidth of ASOPS based TeraHertz Time-Domain Spectroscopy (THz-TDS) systems. We employ two mode-locked femtosecond Ti:Sapphire oscillators with repetition frequencies of 80 MHz operating at a fixed repetition frequency offset of 100 Hz. This offset lock is maintained by a Phase-Locked Loop (PLL) operating at the 60th harmonic of the repetition rate of the Ti:Sapphire oscillators. Their respective time delay is scanned across 12.5 ns requiring a scan time of 10 ms, supporting a time delay resolution of up to 15.6 fs. ASOPS-THz-TDS enables high-resolution spectroscopy that is impossible for a THz-TDS system employing a mechanical delay stage. We measure a timing jitter of 1.36 fs for the system using an air-gap etalon and an optical cross-correlator. We report a Root-Mean-Square deviation of 20.7 MHz and a mean deviation of 14.4 MHz for water absorption lines from 0.5 to 2.7. High-resolution ASOPS-THz-TDS enables high resolution spectroscopy of both gas-phase and condensed-phase samples across a decade of THz bandwidth.

  16. Simultaneous Confocal Scanning Laser Ophthalmoscopy Combined with High-Resolution Spectral-Domain Optical Coherence Tomography: A Review

    PubMed Central

    Castro Lima, Verônica; Rodrigues, Eduardo B.; Nunes, Renata P.; Sallum, Juliana F.; Farah, Michel E.; Meyer, Carsten H.

    2011-01-01

    We aimed to evaluate technical aspects and the clinical relevance of a simultaneous confocal scanning laser ophthalmoscope and a high-speed, high-resolution, spectral-domain optical coherence tomography (SDOCT) device for retinal imaging. The principle of confocal scanning laser imaging provides a high resolution of retinal and choroidal vasculature with low light exposure. Enhanced contrast, details, and image sharpness are generated using confocality. The real-time SDOCT provides a new level of accuracy for assessment of the angiographic and morphological correlation. The combined system allows for simultaneous recordings of topographic and tomographic images with accurate correlation between them. Also it can provide simultaneous multimodal imaging of retinal pathologies, such as fluorescein and indocyanine green angiographies, infrared and blue reflectance (red-free) images, fundus autofluorescence images, and OCT scans (Spectralis HRA + OCT; Heidelberg Engineering, Heidelberg, Germany). The combination of various macular diagnostic tools can lead to a better understanding and improved knowledge of macular diseases. PMID:22132313

  17. Direct Push Optical Screening Tool for High-Resolution, Real-Time Mapping of Chlorinated Solvent DNAPL Architecture

    DTIC Science & Technology

    2016-04-01

    FINAL REPORT Direct Push Optical Screening Tool for High-Resolution, Real- Time Mapping of Chlorinated Solvent DNAPL Architecture ESTCP Project ER...Direct Push Optical Screening Tool for High Resolution, Real-time Mapping of Chlorinated Solvent DNAPL Architecture Murray Einarson Haley...for real-time, high-resolution mapping of petroleum hydrocarbon and coal tar-based NAPL source zones. The objective of this project is to demonstrate

  18. Direct Push Optical Screening Tool for High Resolution, Real-Time Mapping of Chlorinated Solvent DNAPL Architecture

    DTIC Science & Technology

    2016-07-01

    ER-201121) Direct Push Optical Screening Tool for High- Resolution, Real-Time Mapping of Chlorinated Solvent DNAPL Architecture July 2016 This...Report April 2011 - March 2016 Direct Push Optical Screening Tool for High Resolution, Real-time Mapping of Chlorinated Solvent DNAPL Architecture...tools are currently available for real-time, high-resolution mapping of petroleum hydrocarbon and coal tar-based NAPL source zones. The objective of

  19. The Potential for High-Resolution Palaeoclimate Records of the Pliocene from Speleothems

    NASA Astrophysics Data System (ADS)

    Drysdale, R.; Woodhead, J. D.; Hellstrom, J. C.

    2014-12-01

    The Pliocene is widely regarded as a useful analogue for future greenhouse warming and is thus an important interval for palaeoclimate study. Much of what we know about climate through the Pliocene comes from marine sediments. However, we know relatively little about interannual to interdecadal variability, yet reconstructing Pliocene climate at such resolution has the potential to yield important information for testing climate models. Recent advances in uranium-lead (U-Pb) dating have paved the way for investigating Pliocene (and older) high-resolution palaeoclimate records from speleothems. We present such an example from the Nullarbor Plain, Australia. The Nullabor is an emerged, karstified platform of Eocene to Miocene limestones situated on the arid southern fringe of the Australian continent. Caves developed in the karst preserve a large archive of ancient calcite speleothems, in stark contrast to the virtual lack of calcite speleothems today. U-Pb radiometric dating of these speleothems reveals that most the growth occurred during the Pliocene. The geochemistry of these speleothems suggests that the Nullarbor was a wetter and more-vegetated environment at the time. High-resolution geochemical analyses and fluorescence microscopy show that some of the speleothems are almost certainly annually laminated, and contain multi-proxy signatures similar to Holocene speleothems that are commonly associated with hydrological processes. We present a series of encouraging preliminary results from several specimens.

  20. Colonic motor abnormalities in slow transit constipation defined by high resolution, fibre-optic manometry.

    PubMed

    Dinning, P G; Wiklendt, L; Maslen, L; Patton, V; Lewis, H; Arkwright, J W; Wattchow, D A; Lubowski, D Z; Costa, M; Bampton, P A

    2015-03-01

    Slow transit constipation (STC) is associated with colonic motor abnormalities. The underlying cause(s) of the abnormalities remain poorly defined. In health, utilizing high resolution fiber-optic manometry, we have described a distal colonic propagating motor pattern with a slow wave frequency of 2-6 cycles per minute (cpm). A high calorie meal caused a rapid and significant increase in this activity, suggesting the intrinsic slow wave activity could be mediated by extrinsic neural input. Utilizing the same protocol our aim was to characterize colonic meal response STC patients. A fiber-optic manometry catheter (72 sensors at 1 cm intervals) was colonoscopically placed with the tip clipped at the ascending or transverse colon, in 14 patients with scintigraphically confirmed STC. Manometric recordings were taken, for 2 h pre and post a 700 kCal meal. Data were compared to 12 healthy adults. Prior to and/or after the meal the cyclic propagating motor pattern was identified in 13 of 14 patients. However, the meal, did not increase the cyclic motor pattern (preprandial 7.4 ± 7.6 vs postprandial 8.3 ± 4.5 per/2 h), this is in contrast to the dramatic increase observed in health (8.3 ± 13.3 vs 59.1 ± 89.0 per/2 h; p < 0.001). In patients with STC a meal fails to induce the normal increase in the distal colonic cyclic propagating motor patterns. We propose that these data may indicate that the normal extrinsic parasympathetic inputs to the colon are attenuated in these patients. © 2015 John Wiley & Sons Ltd.

  1. Optical design of a versatile FIRST high-resolution near-IR spectrograph

    NASA Astrophysics Data System (ADS)

    Zhao, Bo; Ge, Jian

    2012-09-01

    We report the update optical design of a versatile FIRST high resolution near IR spectrograph, which is called Florida IR Silicon immersion grating spectromeTer (FIRST). This spectrograph uses cross-dispersed echelle design with white pupils and also takes advantage of the image slicing to increase the spectra resolution, while maintaining the instrument throughput. It is an extremely high dispersion R1.4 (blazed angle of 54.74°) silicon immersion grating with a 49 mm diameter pupil is used as the main disperser at 1.4μm -1.8μm to produce R=72,000 while an R4 echelle with the same pupil diameter produces R=60,000 at 0.8μm -1.35μm. Two cryogenic Volume Phase Holographic (VPH) gratings are used as cross-dispersers to allow simultaneous wavelength coverage of 0.8μm -1.8μm. The butterfly mirrors and dichroic beamsplitters make a compact folding system to record these two wavelength bands with a 2kx2k H2RG array in a single exposure. By inserting a mirror before the grating disperser (the SIG and the echelle), this spectrograph becomes a very efficient integral field 3-D imaging spectrograph with R=2,000-4,000 at 0.8μm-1.8μm by coupling a 10x10 telescope fiber bundle with the spectrograph. Details about the optical design and performance are reported.

  2. Construction of a high resolution microscope with conventional and holographic optical trapping capabilities.

    PubMed

    Butterfield, Jacqualine; Hong, Weili; Mershon, Leslie; Vershinin, Michael

    2013-04-22

    High resolution microscope systems with optical traps allow for precise manipulation of various refractive objects, such as dielectric beads (1) or cellular organelles (2,3), as well as for high spatial and temporal resolution readout of their position relative to the center of the trap. The system described herein has one such "traditional" trap operating at 980 nm. It additionally provides a second optical trapping system that uses a commercially available holographic package to simultaneously create and manipulate complex trapping patterns in the field of view of the microscope (4,5) at a wavelength of 1,064 nm. The combination of the two systems allows for the manipulation of multiple refractive objects at the same time while simultaneously conducting high speed and high resolution measurements of motion and force production at nanometer and piconewton scale.

  3. Construction of a High Resolution Microscope with Conventional and Holographic Optical Trapping Capabilities

    PubMed Central

    Butterfield, Jacqualine; Hong, Weili; Mershon, Leslie; Vershinin, Michael

    2013-01-01

    High resolution microscope systems with optical traps allow for precise manipulation of various refractive objects, such as dielectric beads 1 or cellular organelles 2,3, as well as for high spatial and temporal resolution readout of their position relative to the center of the trap. The system described herein has one such "traditional" trap operating at 980 nm. It additionally provides a second optical trapping system that uses a commercially available holographic package to simultaneously create and manipulate complex trapping patterns in the field of view of the microscope 4,5 at a wavelength of 1,064 nm. The combination of the two systems allows for the manipulation of multiple refractive objects at the same time while simultaneously conducting high speed and high resolution measurements of motion and force production at nanometer and piconewton scale. PMID:23629580

  4. InSAR Forensics: Tracing InSAR Scatterers in High Resolution Optical Image

    NASA Astrophysics Data System (ADS)

    Wang, Yuanyuan; Zhu, XiaoXiang

    2015-05-01

    This paper presents a step towards a better interpretation of the scattering mechanism of different objects and their deformation histories in SAR interferometry (InSAR). The proposed technique traces individual SAR scatterer in high resolution optical images where their geometries, materials, and other properties can be better analyzed and classified. And hence scatterers of a same object can be analyzed in group, which brings us to a new level of InSAR deformation monitoring.

  5. High-resolution record of pyrogenic polycyclic aromatic hydrocarbon deposition during the 20th century.

    PubMed

    Lima, Ana Lúcia C; Eglinton, Timothy I; Reddy, Christopher M

    2003-01-01

    A high-resolution record of polycyclic aromatic hydrocarbon (PAH) deposition in Rhode Island over the past approximately 180 years was constructed using a sediment core from the anoxic Pettaquamscutt River basin. The record showed significantly more structure than has hitherto been reported and revealed four distinct maxima in PAH flux. The characteristic increase in PAH flux at the turn of the 20th century was captured in detail, leading to an initial maximum prior to the Great Depression. The overall peak in PAH flux in the 1950s was followed by a maximum that immediately preceded the 1973 Organization of Petroleum Exporting Countries (OPEC) oil embargo. During the most recent portion of the record, an abrupt increase in PAH flux between 1996 and 1999 has been found to follow a period of near constant fluxes. Because source-diagnostic ratios indicate that petrogenic inputs are minor throughout the record, these trends are interpreted in terms of past variations in the magnitude and type of combustion processes. For the most recent PAH maximum, energy consumption data suggest that diesel fuel combustion, and hence traffic of heavier vehicles, is the most probable cause for the increase in PAH flux. Systematic variations in the relative abundance of individual PAHs in conjunction with the above changes in flux are interpreted in relation to the evolution of combustion processes. Coronene, retene, and perylene are notable exceptions, exhibiting unique down-core profiles.

  6. Low-power high-resolution 32-channel neural recording system.

    PubMed

    Yun, Xiao; Kim, Donghwi; Stanaćević, Milutin; Mainen, Zachary

    2007-01-01

    A design of low-power 32-channel neural recording system with on-chip high-resolution A/D converters is presented. A neural front-end including low-noise fully differential pre-amplifier, gain stage, and buffer consumes only 56 mu W. Two 13-bits extended counting A/D converters running at 512KHz sampling rate are integrated with 32 neural front-ends on a chip. The experimental prototype was designed in 0.6 microm CMOS process. With a 3.3V power supply, total power consumption of a chip is 22mW and the whole system occupies an area of 3mm x 3mm.

  7. High-resolution palaeomagnetic records of the Laschamp geomagnetic excursion from ODP Sites 1061 and 1062

    NASA Astrophysics Data System (ADS)

    Bourne, M. D.; Henderson, G. M.; Thomas, A. L.; Mac Niocaill, C.

    2012-12-01

    The Laschamp geomagnetic excursion (~41 ka) was a brief global deviation in geomagnetic field behaviour from that expected during normal secular variation. Previously published records suggest rapid changes in field direction and a concurrent substantial decrease in field intensity. We present here high-resolution palaeomagnetic records of the Laschamp excursion obtained from two Ocean Drilling Program (ODP) Sites 1061 and 1062 on the Blake-Bahama Outer Ridge (ODP Leg 172) and compare this record with previously published records of the Blake and Iceland Basin Excursions. Relatively high sedimentation rates (>10 cm kyr-1) at these locations allow the determination of transitional field behaviour during the excursion. Rather than assuming a constant sedimentation rate between assigned age tie-points, we employ measurements of 230Thxs concentration in the sediment to assess variations in the sedimentation rates through the core sections of interest. This allows us to better determine the temporal behaviour of the Laschamp excursion with greater accuracy and known uncertainty. The Laschamp excursion at this location appears to be much shorter in duration than the Blake and Iceland Basin excursions. Palaeomagnetic measurements of discrete samples from four cores reveal a single excursional feature, across an interval of 30 cm, associated with a broader palaeointensity low. The excursion is characterised by rapid transitions (less than 500 years) between a stable normal polarity and a partially-reversed, polarity. Peaks in inclination either side of the directional excursion indicate periods of time when the local field is dominated by vertical flux patches. Similar behaviour has been observed in records of the Iceland Basin Excursion from the same region. The palaeointensity record is in good agreement between the two sites. The palaeointensity record shows two minima, where the second dip in intensity is associated with a more limited directional deviation. Similar

  8. Reconstruction of a continuous high-resolution CO2 record over the past 20 million years

    NASA Astrophysics Data System (ADS)

    van de Wal, R. S. W.; de Boer, B.; Lourens, L. J.; Köhler, P.; Bintanja, R.

    2011-12-01

    The gradual cooling of the climate during the Cenozoic has generally been attributed to a decrease in CO2 concentration in the atmosphere. The lack of transient climate models and, in particular, the lack of high-resolution proxy records of CO2, beyond the ice-core record prohibit, however, a full understanding of, for example, the inception of the Northern Hemisphere glaciation and mid-Pleistocene transition. Here we elaborate on an inverse modelling technique to reconstruct a continuous CO2 series over the past 20 million year (Myr), by decomposing the global deep-sea benthic δ18O record into a mutually consistent temperature and sea level record, using a set of 1-D models of the major Northern and Southern Hemisphere ice sheets. We subsequently compared the modelled temperature record with ice core and proxy-derived CO2 data to create a continuous CO2 reconstruction over the past 20 Myr. Results show a gradual decline from 450 ppmv around 15 Myr ago to 225 ppmv for mean conditions of the glacial-interglacial cycles of the last 1 Myr, coinciding with a gradual cooling of the global surface temperature of 10 K. Between 13 to 3 Myr ago there is no long-term sea level variation caused by ice-volume changes. We find no evidence of change in the long-term relation between temperature change and CO2, other than the effect following the saturation of the absorption bands for CO2. The reconstructed CO2 record shows that the Northern Hemisphere glaciation starts once the long-term average CO2 concentration drops below 265 ppmv after a period of strong decrease in CO2. Finally, only a small long-term decline of 23 ppmv is found during the mid-Pleistocene transition, constraining theories on this major transition in the climate system. The approach is not accurate enough to revise current ideas about climate sensitivity.

  9. High resolution coral records of reactive and micronutrient trace metals: Monitoring biological responses to flood plumes.

    NASA Astrophysics Data System (ADS)

    Wyndham, T. D.; McCulloch, M. T.; Decarlo, E. H.

    2004-12-01

    The influences of flood plumes on the coastal ocean are difficult to investigate because they are intermittent, transient and highly variable in nature. The application of trace metals in coral carbonates as proxy recorders of marine environmental conditions has been demonstrated as an excellent method for overcoming these difficulties. Coral records of trace metals have been widely used to provide historical records of physical impacts of flood plumes such changing salinity (δ O18), sediment load (Ba) and anthropogenic inputs such as heavy metal pollution (eg. Pb, Cd). Despite successful applications to physical properties, the use of coral records to monitoring the biologic responses to these changing environmental conditions has proved more difficult. With improvements in analytical techniques however, it is now possible to investigate coral records of reactive (rare earth elements) and micronutrient (eg Mn, Zn and Cu) trace metals, which can be used to more widely explore the biogeochemical implications of flood plumes to the coastal ocean. We have obtained high resolution temporal records of rare earth elements, Cu, Zn, Mn and Sn, from corals from two locations, (1) near Townsville on the Great Barrier Reef (GBR) and (2) Kaneohe Bay on Oahu, Hawaii. Both of these locations often display large phytoplankton blooms following flood events, providing a good opportunity to test the idea that coral records can be used to monitor the biological response to flood plumes. These coral records show significant responses to flood plumes that can only be attributed to biogeochemical cycling that occurs as a result of the flood plume influence, rather than the flood plume itself. Thus a mechanism for exploring the biological response to flood plumes is provided. It has generally proven difficult however, to interpret these coral records without a good understanding of the coastal processes. Therefore we also include evidence from direct trace metal measurements of a

  10. In Situ Visualization of Tears on Contact Lens Using Ultra High Resolution Optical Coherence Tomography

    PubMed Central

    Wang, Jianhua; Jiao, Shuliang; Ruggeri, Marco; Shousha, Mohammed Abou; Chen, Qi

    2012-01-01

    Objective To demonstrate the capability of directly visualizing the tear film on contact lenses using optical coherence tomography (OCT). Methods Six eyes of three healthy subjects wearing PureVision and ACUVUE Advance soft and Boston RGP hard contact lenses were imaged with a custom built, high speed, ultra-high resolution spectral domain optical coherence tomograph. Refresh Liquigel was used to demonstrate the effect of artificial tears on the tear film. Results Ultra high resolution images of the pre- and post-lens films were directly visualized when each lens was inserted onto the eye. After the instillation of artificial tears during lens wear, the tear film was thicker. The post-lens tear film underneath the lens edge was clearly shown. Interactions between the lens edges and the ocular surface were obtained for each of the lens types and base curves. With a contrast enhancement agent, tear menisci on the contact lenses around the upper and lower eyelids were highlighted. With hard contact lenses, the tear film was visualized clearly and changed after a blink when the lens was pulled up by the lid. Conclusions Ultra-high resolution OCT is a potentially promising technique for imaging tears around contact lenses. This successful demonstration of in situ post-lens tear film imaging suggests that OCT could open a new era in studying tear dynamics during contact lens wear. The novel method may lead to new ways of evaluating contact lens fitting. PMID:19265323

  11. An ultra-high resolution last deglacial marine sediment records of the Northwest Atlantic Ocean

    NASA Astrophysics Data System (ADS)

    Rashid, H.; Piper, D.; Marche, B.; Vermooten, M.; Lazar, K.; Brockway, B.

    2016-12-01

    Lack of high sedimentation rate records of past changes pertaining to the late Pleistocene Laurentide ice-sheet (LIS) dynamics has prevented efforts to differentiate the various forcings in modulating abrupt climate changes. Here, we present an ultra-high resolution sediment record spanning approximately 1,500 km of the Eastern Canadian continental margin. The new record comprises four sediment cores which were collected from the northwest Labrador Sea (i.e., Saglek Bank) to southwestern Flemish Pass to the southeast Grand Banks in outer shelf and slope settings. Fifty new 14C-accelerator mass spectrometric dates were obtained to construct the stratigraphy. The total sediment thickness of the new record is 41 m covering the past 26 ka with 1.58/ka mean sediment rate, the highest sediment rate ever reported from the Northwest Atlantic Ocean for this time interval. Further, the temporal resolution of the record varies from a couple of decades to centuries depending on the time interval. X-ray fluorescence (XRF) data in conjunction with physical properties of sediments and petrology allowed us to distinguish sediment delivered by major ice-streams of the LIS namely the Hudson Strait, Hopedale Saddle, and Cumberland Sound ice streams. Heinrich layers 1 and 2 are well identified by their Labrador Sea specific characteristics. The so-called Younger Dryas equivalent Heinrich layer H0 was identified in these cores but the timing of onset of H0 has an offset by nearly 1,000 years with that of the 12.9 ka, suggesting that the YD event was not initiated by the Hudson Strait compared to other Heinrich events.

  12. High-resolution approaches to understanding short- and long-term trends in speleothem geochemical records

    NASA Astrophysics Data System (ADS)

    Fairchild, I. J.; Baker, A.; Mattey, D.; Spötl, C.; McDermott, F.; Baldini, L.; McMillan, E. A.; Frisia, S.; Andreo, B.; Borsato, A.

    2005-12-01

    Speleothem geochemical records can display and allow accurate dating of large-scale reorganizations of the climate system. Whilst large shifts in oxygen isotope composition in climatically sensitive (e.g. monsoonal) areas can be confidently interpreted in terms of the nature and sense of change, most more subtle variations cannot be unambiguously interpreted from low-resolution records. The use of high-resolution (sub-annual) records allows the processes involved in change to be more confidently interpreted and examples are given from work in several current European projects. Whilst some cave drip sites are well-mixed through the year, others reveal the seasonality of dripwater composition, and sub-annual oxygen isotope records can record the varying contributions of different seasons. When coupled with improved understanding of meteorological controls on rainfall composition, both the nature and mechanisms of climatic change can be tackled. Carbon isotopic patterns can be strongly influenced by seasonal patterns of cave ventilation that could reveal, e.g. intensity of winter cold. These are superimposed on the better-known vegetation effects. Trace element patterns depend strongly on cave physiology and both seasonal dryness and seasonally varying cave ventilation are key controls in different cases. Caves dominated by seasonal dryness lend themselves to parallel interpretation of longer-term aridity through Sr and Mg trends. In contrast, Alpine caves show interlinked hydrological and cave ventilation controls that can be distinguished by annual patterns of different trace species such as Pb, Y and P (hydrological control) and S (ventilation control). The multi-proxy approach calibrated with reference to modern dripwater behaviour and speleothems forming during the instrumental climatic period provides a sound basis for the interpretation of long-term trends.

  13. Synchronized High-Resolution Lacustrine Records in Iceland show Non-Linear Response to Holocene Insolation

    NASA Astrophysics Data System (ADS)

    Geirsdottir, A.; Miller, G. H.; Larsen, D. J.; Thordarson, T.; Ólafsdóttir, S.; Stoner, J. S.

    2010-12-01

    Icelandic lakes commonly have sedimentation rates in excess of 1 m ka-1 through the Holocene, offering the potential for records of environmental change at decadal or better resolution. Icelandic lake sediment contains numerous volcanic tephra layers of known age, which together with high-resolution sediment paleomagnetic secular variations (PSV) allow synchronization of sediment cores from both lacustrine and marine archives. We present synchronized high-resolution paleoclimatic records from two Icelandic lakes with very different catchment characteristics. By combining PSV records and key tephra tie points we are able to synchronize the lacustrine records with each other and with a well-dated marine core from the shelf north of Iceland. The large PSV signal that characterizes the Icelandic Holocene records allows 40 to 60 secure tie points over the past 10 ka of sediment records. The high frequency of tie points allows the reconstruction of sediment accumulation rate changes in the lacustrine records that were not apparent from the tephrochonological controls. The first order trends in the lacustine climate proxies (BSi and TOC) are similar. BSi climbs to a maximum value shortly after 8 ka, then declines toward present, reflecting a relatively late Holocene thermal maximum, lagging the Greenland ice core record by ca. 2 ka. The peak of the HTM in Iceland was warm enough to melt glaciers completely with temperatures estimated to have been 3.5°C higher relative to 1960-1990 averages. Decreasing summer insolation is reflected not by gradual cooling after the HTM, but by incremental changes in state. TOC and BSi track each other during warm times, but diverge, and sedimentation rates increase, during perturbations and cold times at 8.4 ka, 5.5 ka, 4.3 to 4 ka, 3.1 ka to 2.8 ka. Following these departures, BSi usually exhibits a step-function change, re-equilibrating at a lower BSi value. Some of the departures may be related to Icelandic volcanism influencing

  14. Development of a high resolution optical-fiber tilt sensor by F-P filter

    NASA Astrophysics Data System (ADS)

    Pan, Jianjun; Nan, Qiuming; Li, Shujie; Hao, Zhonghua

    2017-04-01

    A high-resolution tilt sensor is developed, which is composed of a pair of optical fiber collimators and a simple pendulum with an F-P filter. The tilt angle is measured by demodulating the shift of center wavelength of F-P filter, which is caused by incidence angle changing. The relationship between tilted angle and the center wavelength is deduced. Calibration experiment results also confirm the deduction, and show that it is easy to obtain a high resolution. Setting the initial angle to 6degree, the measurement range is ±3degree, its average sensitivity is 1104pm/degree, and its average resolution is as high as 0.0009degree.

  15. High-resolution and wide-bandwidth light intensity fiber optic displacement sensor for MEMS metrology.

    PubMed

    Orłowska, Karolina; Świątkowski, Michał; Kunicki, Piotr; Kopiec, Daniel; Gotszalk, Teodor

    2016-08-01

    We report on the design, properties, and applications of a high-resolution and wide-bandwidth light intensity fiber optic displacement sensor for microelectromechanical system (MEMS) metrology. There are two types of structures that the system is dedicated to: vibrating with both high and low frequencies. In order to ensure high-frequency and high-resolution measurements, frequency down mixing and selective signal processing were applied. The obtained effective measuring bandwidth ranges from single hertz to 1 megahertz. The achieved resolution presented here is 116  pm/Hz1/2 and 138  pm/Hz1/2 for low-frequency and high-frequency operation modes, respectively, whereas the measurement of static displacement is 100 μm.

  16. Far-field high resolution effects and manipulating of electromagnetic waves based on transformation optics

    NASA Astrophysics Data System (ADS)

    Ji, XueBin; Zang, XiaoFei; Li, Zhou; Shi, Cheng; Chen, Lin; Cai, Bin; Zhu, YiMing

    2015-05-01

    Based on the transformation optics (TO) and the effective medium theory (EMT), a new illusion media with homogeneous and isotropic materials is proposed to realize the far-field high resolution effects. When two point sources with the separation distance of λ0 / 4 are covered with the illusion media (λ0 is the free-space wavelength), the corresponding far-field pattern is equivalent to the case of two point sources with the separation distance larger than λ0 / 2 in free space, leading to the far-field high resolution effects (in free space, the separation distance of λ0 / 4 is less than half-wavelength, and thus the two point sources cannot be distinguished from each other). Furthermore, such illusion media can be applied to design tunable high-directivity antenna and an angle-dependent floating carpet cloak. Full wave simulations are carried out to verify the performance of our device.

  17. Adaptive optics high-resolution IR spectroscopy with silicon grisms and immersion gratings

    NASA Astrophysics Data System (ADS)

    Ge, Jian; McDavitt, Daniel L.; Chakraborty, Abhijit; Bernecker, John L.; Miller, Shane

    2003-02-01

    The breakthrough of silicon immersion grating technology at Penn State has the ability to revolutionize high-resolution infrared spectroscopy when it is coupled with adaptive optics at large ground-based telescopes. Fabrication of high quality silicon grism and immersion gratings up to 2 inches in dimension, less than 1% integrated scattered light, and diffraction-limited performance becomes a routine process thanks to newly developed techniques. Silicon immersion gratings with etched dimensions of ~ 4 inches are being developed at Penn State. These immersion gratings will be able to provide a diffraction-limited spectral resolution of R = 300,000 at 2.2 micron, or 130,000 at 4.6 micron. Prototype silicon grisms have been successfully used in initial scientific observations at the Lick 3m telescope with adaptive optics. Complete K band spectra of a total of 6 T Tauri and Ae/Be stars and their close companions at a spectral resolution of R ~ 3000 were obtained. This resolving power was achieved by using a silicon echelle grism with a 5 mm pupil diameter in an IR camera. These results represent the first scientific observations conducted by the high-resolution silicon grisms, and demonstrate the extremely high dispersing power of silicon-based gratings. New discoveries from this high spatial and spectral resolution IR spectroscopy will be reported. The future of silicon-based grating applications in ground-based AO IR instruments is promising. Silicon immersion gratings will make very high-resolution spectroscopy (R > 100,000) feasible with compact instruments for implementation on large telescopes. Silicon grisms will offer an efficient way to implement low-cost medium to high resolution IR spectroscopy (R ~ 1000-50000) through the conversion of existing cameras into spectrometers by locating a grism in the instrument's pupil location.

  18. A geochemical high-resolution record of late Holocene droughts from western-central Mexico

    NASA Astrophysics Data System (ADS)

    Sosa, S.; Lozano-Garcia, S.; Roy, P.; Caballero, M.

    2013-05-01

    We present a model of change in precipitation over the late Holocene from Santa Maria del Oro Lake (SMO) located at the western end of central Mexico. The present day precipitation in the region is associated mainly to the Mexican Monsoon and ENSO activity. This tropical site is characterized by 7 to 8 month long dry season between October and May. The model is based on high-resolution Titanium and Calcium concentrations data obtained by an ITRAX XRF core scanner and magnetic susceptibility of two 14C dated lacustrine sequences from the crater lake of SMO. The Ti record is a proxy indicator of runoff into the basin and we validated this record by comparing it with registers of historical droughts and tree ring chronologies. The geochemical data documents 4 different intervals of drought (i.e. 4000-3500, 3100-2050, 1750-1000 cal yr BP and last 120 years) and 3 wet periods (3500-3100, 2050-1750 and 1000-120 cal yr BP). The droughts were of different intensities and we identified a major drought during the Classic (0 to 1000 AD) with gradual increase in aridity towards the Epiclassic (800 to 1000 AD) that coincides with the Maya drought. The drought record at Santa Maria del Oro is compared with a record from the Cariaco basin indicating the latitudinal displacement of ITCZ and with the El Junco register of ENSO. Spectral analysis of the data indicates 50-year periodicities possibly related to solar activity and climatic forcings such as ENSO.

  19. High-resolution record of the Laschamp geomagnetic excursion at the Blake-Bahama Outer Ridge

    NASA Astrophysics Data System (ADS)

    Bourne, Mark D.; Mac Niocaill, Conall; Thomas, Alex L.; Henderson, Gideon M.

    2013-12-01

    Geomagnetic excursions are brief deviations of the geomagnetic field from behaviour expected during `normal secular' variation. The Laschamp excursion at ˜41 ka was one such deviation. Previously published records suggest rapid changes in field direction and a concurrent substantial decrease in field intensity associated with this excursion. Accurate dating of excursions, and determination of their durations from multiple locations, is vital to our understanding of global field behaviour during these deviations. We present here high-resolution palaeomagnetic records of the Laschamp excursion obtained from two Ocean Drilling Program (ODP) Sites, 1061 and 1062 on the Blake-Bahama Outer Ridge (ODP Leg 172). High sedimentation rates (˜30-40 cm kyr-1) at these locations allow determination of transitional field behaviour during the excursion. Palaeomagnetic measurements of discrete samples from four cores reveal a single excursional feature, across an interval of 30 cm, associated with a broader palaeointensity low. We determine the age and duration of the Laschamp excursion using a stratigraphy linked to the δ18O record from the Greenland ice cores. This chronology dates the Laschamp excursion at the Blake Ridge to 41.3 ka. The excursion is characterized by rapid transitions (less than 200 yr) between stable normal polarity and a partially reversed polarity state. The palaeointensity record is in good agreement between the two sites, revealing two prominent minima. The first minimum is associated with the Laschamp excursion at 41 ka and the second corresponds to the Mono Lake excursion at ˜35.5 ka. We determine that the directional excursion during the Laschamp at this location was no longer than ˜400 yr, occurring within a palaeointensity minimum that lasted 2000 yr. The Laschamp excursion at this location is much shorter in duration than the Blake and Iceland Basin excursions.

  20. Low Frequency Error Analysis and Calibration for High-Resolution Optical Satellite's Uncontrolled Geometric Positioning

    NASA Astrophysics Data System (ADS)

    Wang, Mi; Fang, Chengcheng; Yang, Bo; Cheng, Yufeng

    2016-06-01

    The low frequency error is a key factor which has affected uncontrolled geometry processing accuracy of the high-resolution optical image. To guarantee the geometric quality of imagery, this paper presents an on-orbit calibration method for the low frequency error based on geometric calibration field. Firstly, we introduce the overall flow of low frequency error on-orbit analysis and calibration, which includes optical axis angle variation detection of star sensor, relative calibration among star sensors, multi-star sensor information fusion, low frequency error model construction and verification. Secondly, we use optical axis angle change detection method to analyze the law of low frequency error variation. Thirdly, we respectively use the method of relative calibration and information fusion among star sensors to realize the datum unity and high precision attitude output. Finally, we realize the low frequency error model construction and optimal estimation of model parameters based on DEM/DOM of geometric calibration field. To evaluate the performance of the proposed calibration method, a certain type satellite's real data is used. Test results demonstrate that the calibration model in this paper can well describe the law of the low frequency error variation. The uncontrolled geometric positioning accuracy of the high-resolution optical image in the WGS-84 Coordinate Systems is obviously improved after the step-wise calibration.

  1. High-resolution stable carbon isotope record of the Permian to earliest Triassic from East Greenland

    NASA Astrophysics Data System (ADS)

    Sanson Barrera, Anna; Hochuli, Peter A.; Bucher, Hugo; Meier, Maximiliano; Schneebeli Hermann, Elke; Weissert, Helmut; Bernasconi, Stefano M.

    2013-04-01

    The Late Permian and Early Triassic organic carbon isotope records show global major excursions probably triggered by episodic volcanic degasing of the Siberian Large Igneous Province. Important and rapid fluctuations of the global carbon cycle are also reflected in the biosphere. The geological record seems to comprise several major floral and marine faunal turnovers indicating short-lived biotic recoveries. In northwest Pangea, the active Early Triassic Greenland - Norway rifting system led to the accommodation of thick sedimentary sequences. This basin has a great potential for detailed studies of regional and global biotic and climatic changes with high temporal resolution during this critical interval in Earth's history. The western part of this basin is exposed in north-eastern Greenland and is represented by a succession of deltaic sediments organized in a general regressive trend ranging throughout the Griesbachian and the onset of the Dienerian. On the eastern side of the basin the succession has been drilled off the Norwegian coast. On Hold with Hope (East Greenland, 74°N) up to ca. 800m thick sections of the ammonoid-bearing Early Triassic Wordie Creek Formation have been logged and sampled. Here we present a high-resolution organic carbon isotope record and preliminary palynofacies data of a 500m thick composite section ranging from the Permian into the earliest Triassic. The organic carbon isotope record is closely comparable to the coeval section from the Trøndelag platform in Mid-Norway. The two records show a first major negative shift (ca. -6‰) representing the unconformity between the Ravnefjeld and the Wordie Creek formations, regionally known as the lithological Permian-Triassic boundary. Higher up, a second negative shift of ca. -4‰ correlates with the carbon shift associated with the GSSP Permian-Triassic boundary as defined at Meishan (China), represented by carbon isotope values around -30‰. This negative shift is followed by a

  2. A High-Resolution Porphyrin Nitrogen Isotope Record of an Oceanic Anoxic Event

    NASA Astrophysics Data System (ADS)

    Pearson, A.; Higgins, M. B.; Robinson, R. S.; Carter, S. J.

    2010-12-01

    Nitrogen isotope values measured in sediments deposited at times of widespread marine suboxic conditions are consistently more depleted in 15N than are modern marine sediments. We measured a high-resolution δ15N record of sedimentary porphyrins from a section spanning the Cenomanian-Turonian Oceanic Anoxic Event (OAE) II to examine changes in the nitrogen cycle recorded in surface waters. We compare these values to δ15N values of three sedimentary fractions: bulk sediment, extractable organic matter (bitumen), and non-extractable organic matter (kerogen). All fractions record similar trends; are offset from each other by consistent differences that are reflective of algal (not cyanobacterial) export; and are depleted in 15N compared to modern sedimentary samples, both before, during, and after the interval defined by the OAE. Prior to the termination of OAE II, there is a slight further negative excursion in δ15N values. In order to interpret these and other depleted δ15N values measured in similar black shales, we invoke a nitrogen isotope model that considers the importance of redox transformations of N under suboxic conditions. This model invokes an ocean in which the deep N reservoir is dominated by NH4+, limited nitrification occurs in the photic zone, and chemocline denitrification acts as a quantitative sink for downwelling nitrate. Although in this model a large component of net production may be fueled by N fixation, N fixation alone cannot generate biomass with δ15N values as depleted as are seen in many Mesozoic OAE sections. We postulate that throughout the Phanerozoic, excursions leading to negative values of d15N reflect relatively low rates of nitrosification relative to the sum of NH4+ utilization and N fixation.

  3. Inter- and intrarater reliability of the Chicago Classification in pediatric high-resolution esophageal manometry recordings.

    PubMed

    Singendonk, M M J; Smits, M J; Heijting, I E; van Wijk, M P; Nurko, S; Rosen, R; Weijenborg, P W; Abu-Assi, R; Hoekman, D R; Kuizenga-Wessel, S; Seiboth, G; Benninga, M A; Omari, T I; Kritas, S

    2015-02-01

    The Chicago Classification (CC) facilitates interpretation of high-resolution manometry (HRM) recordings. Application of this adult based algorithm to the pediatric population is unknown. We therefore assessed intra and interrater reliability of software-based CC diagnosis in a pediatric cohort. Thirty pediatric solid state HRM recordings (13M; mean age 12.1 ± 5.1 years) assessing 10 liquid swallows per patient were analyzed twice by 11 raters (six experts, five non-experts). Software-placed anatomical landmarks required manual adjustment or removal. Integrated relaxation pressure (IRP4s), distal contractile integral (DCI), contractile front velocity (CFV), distal latency (DL) and break size (BS), and an overall CC diagnosis were software-generated. In addition, raters provided their subjective CC diagnosis. Reliability was calculated with Cohen's and Fleiss' kappa (κ) and intraclass correlation coefficient (ICC). Intra- and interrater reliability of software-generated CC diagnosis after manual adjustment of landmarks was substantial (mean κ = 0.69 and 0.77 respectively) and moderate-substantial for subjective CC diagnosis (mean κ = 0.70 and 0.58 respectively). Reliability of both software-generated and subjective diagnosis of normal motility was high (κ = 0.81 and κ = 0.79). Intra- and interrater reliability were excellent for IRP4s, DCI, and BS. Experts had higher interrater reliability than non-experts for DL (ICC = 0.65 vs ICC = 0.36 respectively) and the software-generated diagnosis diffuse esophageal spasm (DES, κ = 0.64 vs κ = 0.30). Among experts, the reliability for the subjective diagnosis of achalasia and esophageal gastric junction outflow obstruction was moderate-substantial (κ = 0.45-0.82). Inter- and intrarater reliability of software-based CC diagnosis of pediatric HRM recordings was high overall. However, experience was a factor influencing the diagnosis of some motility disorders, particularly DES and achalasia. © 2014 John Wiley & Sons

  4. Ultra-high resolution spectral domain optical coherence tomography using supercontinuum light source

    NASA Astrophysics Data System (ADS)

    Lim, Yiheng; Yatagai, Toyohiko; Otani, Yukitoshi

    2016-04-01

    An ultra-high resolution spectral domain optical coherence tomography (SD-OCT) was developed using a cost-effective supercontinuum laser. A spectral filter consists of a dispersive prism, a cylindrical lens and a right-angle prism was built to transmit the wavelengths in range 680-940 nm to the OCT system. The SD-OCT has achieved 1.9 μm axial resolution and the sensitivity was estimated to be 91.5 dB. A zero-crossing fringes matching method which maps the wavelengths to the pixel indices of the spectrometer was proposed for the OCT spectral calibration. A double sided foam tape as a static sample and the tip of a middle finger as a biological sample were measured by the OCT. The adhesive and the internal structure of the foam of the tape were successfully visualized in three dimensions. Sweat ducts was clearly observed in the OCT images at very high resolution. To the best of our knowledge, this is the first demonstration of ultra-high resolution visualization of sweat duct by OCT.

  5. A flux-calibrated, high-resolution atlas of optical sky emission from UVES

    NASA Astrophysics Data System (ADS)

    Hanuschik, R. W.

    2003-09-01

    This paper presents a flux-calibrated, high-resolution, high-SNR atlas of optical and near-IR sky emission. It provides a complete template of the high-resolution night-sky emission spectrum with the deepest exposures ever obtained from the ground. The data have been acquired by UVES, ESO's echelle spectrograph at the 8.2-m UT2 telescope of the Very Large Telescope (VLT). Raw data stacks with up to 16 hours of integration time have been combined. The spectrum covers the range 3140-10 430 Å at a resolving power of about 45 000. A total of 2810 sky emission lines have been measured. This high-resolution spectrum is intended to be used for the identification of previously unknown faint sky lines, for simulations of ground based observations where the sky background is important, as a template for checks on the accuracy and stability of the wavelength scale, and as a reference for the reduction of spectra of faint objects. Figures 7-41 are only available in electronic form at http://www.edpsciences.org Tables 4-9 are only available in electronic form at the CDS via anonymous ftp to cdsarc.u-strasbg.fr (130.79.128.5) or via http:/ /cdsweb.u-strasbg.fr/cgi -bin/qcat?J/A+A/407/1157 Based on observations obtained with the VLT at the European Southern Observatory, Paranal, Chile.

  6. A High-resolution Multi-wavelength Simultaneous Imaging System with Solar Adaptive Optics

    NASA Astrophysics Data System (ADS)

    Rao, Changhui; Zhu, Lei; Gu, Naiting; Rao, Xuejun; Zhang, Lanqiang; Bao, Hua; Kong, Lin; Guo, Youming; Zhong, Libo; Ma, Xue’an; Li, Mei; Wang, Cheng; Zhang, Xiaojun; Fan, Xinlong; Chen, Donghong; Feng, Zhongyi; Wang, Xiaoyun; Wang, Zhiyong

    2017-10-01

    A high-resolution multi-wavelength simultaneous imaging system from visible to near-infrared bands with a solar adaptive optics system, in which seven imaging channels, including the G band (430.5 nm), the Na i line (589 nm), the Hα line (656.3 nm), the TiO band (705.7 nm), the Ca ii IR line (854.2 nm), the He i line (1083 nm), and the Fe i line (1565.3 nm), are chosen, is developed to image the solar atmosphere from the photosphere layer to the chromosphere layer. To our knowledge, this is the solar high-resolution imaging system with the widest spectral coverage. This system was demonstrated at the 1 m New Vaccum Solar Telescope and the on-sky high-resolution observational results were acquired. In this paper, we will illustrate the design and performance of the imaging system. The calibration and the data reduction of the system are also presented.

  7. Comparison of three-dimensional optical coherence tomography and high resolution photography for art conservation studies.

    PubMed

    Adler, Desmond C; Stenger, Jens; Gorczynska, Iwona; Lie, Henry; Hensick, Teri; Spronk, Ron; Wolohojian, Stephan; Khandekar, Narayan; Jiang, James Y; Barry, Scott; Cable, Alex E; Huber, Robert; Fujimoto, James G

    2007-11-26

    Gold punchwork and underdrawing in Renaissance panel paintings are analyzed using both three-dimensional swept source / Fourier domain optical coherence tomography (3D-OCT) and high resolution digital photography. 3D-OCT can generate en face images with micrometer-scale resolutions at arbitrary sectioning depths, rejecting out-of-plane light by coherence gating. Therefore 3D-OCT is well suited for analyzing artwork where a surface layer obscures details of interest. 3D-OCT also enables cross-sectional imaging and quantitative measurement of 3D features such as punch depth, which is beneficial for analyzing the tools and techniques used to create works of art. High volumetric imaging speeds are enabled by the use of a Fourier domain mode locked (FDML) laser as the 3D-OCT light source. High resolution infrared (IR) digital photography is shown to be particularly useful for the analysis of underdrawing, where the materials used for the underdrawing and paint layers have significantly different IR absrption properties. In general, 3D-OCT provides a more flexible and comprehensive analysis of artwork than high resolution photography, but also requires more complex instrumentation and data analysis.

  8. Contrast enhanced high-resolution diffuse optical tomography of the human brain using ICG

    PubMed Central

    Habermehl, Christina; Schmitz, Christoph H.; Steinbrink, Jens

    2011-01-01

    Non-invasive diffuse optical tomography (DOT) of the adult brain has recently been shown to improve the spatial resolution for functional brain imaging applications. Here we show that high-resolution (HR) DOT is also advantageous for clinical perfusion imaging using an optical contrast agent. We present the first HR-DOT results with a continuous wave near infrared spectroscopy setup using a dense grid of optical fibers and indocyanine green (ICG) as an exogenic contrast agent. We find an early arrival of the ICG bolus in the intracerebral tissue and a delayed arrival of the bolus in the extracerebral tissue, achieving the separation of both layers. This demonstrates the method’s potential for brain perfusion monitoring in neurointensive care patients. PMID:21935232

  9. All-optical endoscopic probe for high resolution 3D photoacoustic tomography

    NASA Astrophysics Data System (ADS)

    Ansari, R.; Zhang, E.; Desjardins, A. E.; Beard, P. C.

    2017-03-01

    A novel all-optical forward-viewing photoacoustic probe using a flexible coherent fibre-optic bundle and a Fabry- Perot (FP) ultrasound sensor has been developed. The fibre bundle, along with the FP sensor at its distal end, synthesizes a high density 2D array of wideband ultrasound detectors. Photoacoustic waves arriving at the sensor are spatially mapped by optically scanning the proximal end face of the bundle in 2D with a CW wavelength-tunable interrogation laser. 3D images are formed from the detected signals using a time-reversal image reconstruction algorithm. The system has been characterized in terms of its PSF, noise-equivalent pressure and field of view. Finally, the high resolution 3D imaging capability has been demonstrated using arbitrary shaped phantoms and duck embryo.

  10. High-Resolution Spectroscopy and Optical Photometry of MWC 349A and MWC 349B

    NASA Astrophysics Data System (ADS)

    Manset, N.; Miroshnichenko, A. S.; Zharikov, S. V.; Kusakin, A. V.

    2017-02-01

    MWC 349A is a V ˜ 13 mag object with the B[e] phenomenon, a very strong optical emission-line spectrum, maser and laser line emission, and a radio-bright bipolar nebula, attenuated by ˜10 mag of interstellar extinction. MWC 349B is a visual companion of MWC 349A with no previously reported signs of emission. The physical connection of the pair has been the subject of debates toward revealing the evolutionary state of MWC 349A. Only low-resolution spectra reported for MWC 349B resulted in estimating its parameters with a large uncertainty. We report the results of high-resolution optical spectroscopy of both objects as well as their optical photometry.

  11. High-Resolution "Fleezers": Dual-Trap Optical Tweezers Combined with Single-Molecule Fluorescence Detection.

    PubMed

    Whitley, Kevin D; Comstock, Matthew J; Chemla, Yann R

    2017-01-01

    Recent advances in optical tweezers have greatly expanded their measurement capabilities. A new generation of hybrid instrument that combines nanomechanical manipulation with fluorescence detection-fluorescence optical tweezers, or "fleezers"-is providing a powerful approach to study complex macromolecular dynamics. Here, we describe a combined high-resolution optical trap/confocal fluorescence microscope that can simultaneously detect sub-nanometer displacements, sub-piconewton forces, and single-molecule fluorescence signals. The primary technical challenge to these hybrid instruments is how to combine both measurement modalities without sacrificing the sensitivity of either one. We present general design principles to overcome this challenge and provide detailed, step-by-step instructions to implement them in the construction and alignment of the instrument. Lastly, we present a set of protocols to perform a simple, proof-of-principle experiment that highlights the instrument capabilities.

  12. Optimal design of an earth observation optical system with dual spectral and high resolution

    NASA Astrophysics Data System (ADS)

    Yan, Pei-pei; Jiang, Kai; Liu, Kai; Duan, Jing; Shan, Qiusha

    2017-02-01

    With the increasing demand of the high-resolution remote sensing images by military and civilians, Countries around the world are optimistic about the prospect of higher resolution remote sensing images. Moreover, design a visible/infrared integrative optic system has important value in earth observation. Because visible system can't identify camouflage and recon at night, so we should associate visible camera with infrared camera. An earth observation optical system with dual spectral and high resolution is designed. The paper mainly researches on the integrative design of visible and infrared optic system, which makes the system lighter and smaller, and achieves one satellite with two uses. The working waveband of the system covers visible, middle infrared (3-5um). Dual waveband clear imaging is achieved with dispersive RC system. The focal length of visible system is 3056mm, F/# is 10.91. And the focal length of middle infrared system is 1120mm, F/# is 4. In order to suppress the middle infrared thermal radiation and stray light, the second imaging system is achieved and the narcissus phenomenon is analyzed. The system characteristic is that the structure is simple. And the especial requirements of the Modulation Transfer Function (MTF), spot, energy concentration, and distortion etc. are all satisfied.

  13. Texture Analysis Based Fusion Experiments Using High-Resolution SAR and Optical Imagery

    NASA Astrophysics Data System (ADS)

    Zhao, S.; Luo, Y.; Zhou, H.; Xue, Q.; Wang, A.

    2012-08-01

    High resolution SAR images contain plenty of detailed textural features, and optical images have spectral features. For the different characteristics of the two images, Firstly, we extracted textural measures of TerraSAR-X image based on the Gray Level Co-occurrence Matrix (GLCM) method, and chose the appropriate window. Then data fusion between textural measures of TerraSAR-X image and QuickBird multi-spectral image was implemented based on PCA transform, and the fusion results were quantitatively evaluated, showing that the fusion image keep spectral information well and the spatial information be enhanced.

  14. High resolution optical spectroscopy of air-induced electrical instabilities in n-type polymer semiconductors.

    PubMed

    Di Pietro, Riccardo; Sirringhaus, Henning

    2012-07-03

    We use high-resolution charge-accumulation optical spectroscopy to measure charge accumulation in the channel of an n-type organic field-effect transistor. We monitor the degradation of device performance in air, correlate the onset voltage shift with the reduction of charge accumulated in the polymer semiconductor, and explain the results in view of the redox reaction between the polymer, water and oxygen in the accumulation layer. Copyright © 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

  15. High Resolution Spectroscopy of Naphthalene Calibrated by AN Optical Frequency Comb

    NASA Astrophysics Data System (ADS)

    Nishiyama, Akiko; Nakashima, Kazuki; Matsuba, Ayumi; Misono, Masatoshi

    2015-06-01

    In high-resolution molecular spectroscopy, the precise measure of the optical frequency is crucial to evaluate minute shifts and splittings of the energy levels. On the other hand, in such spectroscopy, thousands of spectral lines distributed over several wavenumbers have to be measured by a continuously scanning cw laser. Therefore, the continuously changing optical frequency of the scanning laser has to be determined with enough precision. To satisfy these contradictory requirements, we have been developed two types of high-resolution spectroscopic systems employing an optical frequency comb. One of the systems employs RF band-pass filters to generate equally spaced frequency markers for optical frequency calibration, and is appropriate for wide wavelength-range measurement with relatively high scanning rate.^a In the other system, the beat frequency between the optical frequency comb and the scanning laser is controlled by an acousto-optic frequency shifter. This system is suitable for more precise measurement, and enables detailed analyses of frequency characteristics of scanning laser.^b In the present study, we observe Doppler-free two-photon absorption spectra of A^1B1u (v_4 = 1) ← X^1A_g (v = 0) transition of naphthalene around 298 nm. The spectral lines are rotationally resolved and the resolution is about 100 kHz. For ^qQ transition, the rotational lines are assigned, and molecular constants in the excited state are determined. In addition, we analyze the origin of the measured linewidth and Coriolis interactions between energy levels. To determine molecular constants more precisely, we proceed to measure and analyze spectra of other transitions, such as ^sS transitions. ^a A. Nishiyama, D. Ishikawa, and M. Misono, J. Opt. Soc. Am. B 30, 2107 (2013). ^b A. Nishiyama, A. Matsuba, and M. Misono, Opt. Lett. 39, 4923 (2014).

  16. Q-Spec: a concept for the Giant Magellan Telescope high resolution optical spectrograph

    NASA Astrophysics Data System (ADS)

    Barnes, S.; MacQueen, P.

    2008-07-01

    Q-Spec is a concept for the Giant Magellan Telescope High Resolution Optical Spectrograph. It is a seeing limited, four channel spectrograph designed for high efficiency, high resolution spectroscopy from 305 to 1060 nm. Overall instrument dimensions are minimized with anamorphic preslit optics, pupil slicing, and white pupil beam demagnification. Q-spec uses two 300 × 1600mm R4 echelle grating mosaics of either 2 or 4 individual gratings, with 41.6 and 31.6 grooves/mm line densities. Two beam sizes are selectable in the preslit optics. A 450mm beam yields Rφ = 30,000 while a 900mm beam reaches Rφ = 60,000. Both beams are anamorphised before echelle dispersion, and the larger beam is pupil sliced. The post-echelle white pupil transfer optics demagnify the beam by 3. This allows the use of efficient vph grating cross-dispersers, and unvignetted f/1.5-2.0 dioptric cameras with optical element diameters under 250 mm. The bandwidth is split by a dichroic prior to the entrance slit, and by dichroics near the intermediate foci of the two sets of white pupil transfer optics. The four fixed spectral formats have 2-pixel resolving powers of ~200,000 and it is anticipated that resolving powers of R = 150, 000 or greater will be possible. The largest ccd is 6k × 6k with 15 μm pixels, and the minimum order separation is around 10 arcseconds. Q-spec can be fed with fibers in either multiple-object and/or precision radial velocity modes. Excluding the gravity-invariant thermal and vacuum enclosures, the instrument volume is a modest 5 × 2.5 × 2m in size.

  17. High-resolution speleothem record of precipitation from the Yucatan Peninsula spanning the Maya Preclassic Period

    NASA Astrophysics Data System (ADS)

    Medina-Elizalde, Martín; Burns, Stephen J.; Polanco-Martínez, Josué M.; Beach, Timothy; Lases-Hernández, Fernanda; Shen, Chuan-Chou; Wang, Hao-Cheng

    2016-03-01

    We produced a new high-resolution absolute U-Th dated stalagmite oxygen isotope record (δ18O) from Río Secreto, Playa del Carmen, Yucatan Peninsula (YP). This new 1434-year stalagmite record (named Itzamna after the Maya god of creation) spans the time interval between BCE 1037 and CE 397 with an average resolution of 8 ± 2 years. It provides a novel view of climate evolution over the Preclassic and early Classic periods in Maya history. To understand the controls of regional precipitation δ18O on seasonal time scales, we characterized the amount effect between precipitation amount (P) and precipitation δ18O (δP). We found that precipitation δ18O in the Yucatan Peninsula is controlled by the amount effect on seasonal scales (δP/ΔP = - 0.0137 ± 0.0031‰ per mm, r = 0.9), as suspected but never before demonstrated. Cave drip δ18O is consistent with the annual amount-weighted δ18O composition of precipitation. Multiple lines of evidence suggest that stalagmite δ18O reflects isotopic equilibrium conditions and thus stalagmite δ18O changes are interpreted to reflect precipitation amount. We determined quantitative precipitation changes from the stalagmite δ18O record following previous methods (Medina-Elizalde and Rohling, 2012). The stalagmite precipitation record suggests twelve periods of anomalous precipitation reductions ranging between about 30 and 70% below mean conditions at the time and with durations from 6 years to 31 years. Between BCE 520 and 166, the speleothem precipitation record suggests that the YP experienced an interval of high precipitation labeled the Late Preclassic Humid Period (LPHP) with precipitation maxima of up to + 86 ± 20%. Preclassic Maya cultural expansion in El Mirador Basin, located in northern Guatemala, took place while the peninsula transitioned from the LPHP to an interval with below average precipitation. We find that the Preclassic abandonment of major centers in the Mirador Basin and others around the Maya

  18. High resolution Holocene sea ice records from Herald Canyon, Chukchi Sea

    NASA Astrophysics Data System (ADS)

    Pearce, Christof; Jakobsson, Martin; O'Regan, Matt; Rattray, Jayne; Barrientos, Natalia; Muchitiello, Francesco; Smittenburg, Rienk; Cronin, Tom; Coxall, Helen; Semiletov, Igor

    2016-04-01

    Arctic Ocean sea ice plays a critical role in the Earth's climate system because of the positive ice-albedo feedback mechanisms as well as its control on ocean-atmospheric heat exchange and potential influence on the thermohaline circulation. Key to improving our understanding of Arctic sea ice cover and its reaction to external forcing is the reconstruction of past variability through paleo-records such as marine sediment cores. Although the observed recent sea ice loss seems to be the strongest of the last millennia, it is still uncertain whether the shift from perennial to seasonal ice cover expected for the near future was unprecedented during the current interglacial. High resolution sea ice reconstructions from the Arctic Ocean are rare, and specifically records from the Russian Arctic are underrepresented. In this study, we present results from marine sediment cores from the Herald Canyon in the East Siberian Sea. The area is one of the major conduits of Pacific water entering the Arctic Ocean basin from the Bering Strait and is thus an ideal place to study past variability of the inflow of these nutrient rich waters. Radiocarbon dating of mollusks indicates very high sedimentation rates at the coring sites which allowed for analyses at centennial resolution up to decadal resolution in the late Holocene. Core samples were analyzed for the biomarker IP25, which is produced by diatoms living in sea ice and is used as a proxy of past seasonal sea ice concentrations. Preliminary results indicate the presence of seasonal sea ice during the entire Late Holocene and show a significant increase of sea ice concentrations during the last millennia.

  19. High resolution Holocene sea ice records from Herald Canyon, East Siberian Sea

    NASA Astrophysics Data System (ADS)

    Pearce, C.; Rattray, J.; Jakobsson, M.; Barrientos, N.; Muschitiello, F.; Smittenberg, R.; O'Regan, M.; Coxall, H.

    2015-12-01

    Arctic Ocean sea ice plays a critical role in the Earth's climate system because of the positive ice-albedo feedback mechanisms as well as its control on ocean-atmospheric heat exchange and potential influence on the thermohaline circulation. Key to improving our understanding of Arctic sea ice cover and its reaction to external forcing is the reconstruction of past variability through paleo-records such as marine sediment cores. Although the observed recent sea ice loss seems to be the strongest of the last millennia, it is still uncertain whether the shift from perennial to seasonal ice cover expected for the near future was unprecedented during the current interglacial. High resolution sea ice reconstructions from the Arctic Ocean are rare, and specifically records from the Russian Arctic are underrepresented. In this study, we present results from marine sediment cores from the Herald Canyon in the East Siberian Sea. The area is one of the major conduits of Pacific water entering the Arctic Ocean basin from the Bering Strait and is thus an ideal place to study past variability of the inflow of these nutrient rich waters. Radiocarbon dating of mollusks indicates very high sedimentation rates at the coring sites which allowed for analyses at centennial resolution up to decadal resolution in the late Holocene. Core samples were analyzed for the biomarker IP25, which is produced by diatoms living in sea ice and is used as a proxy of past seasonal sea ice concentrations. Preliminary results indicate the presence of seasonal sea ice during the entire Late Holocene and show a significant increase of sea ice concentrations during the last millennia.

  20. High resolution Holocene paleomagnetic secular variation records from Bay of Bengal

    NASA Astrophysics Data System (ADS)

    Usapkar, A.; Dewangan, P.; Badesab, F. K.; Mazumdar, A.; Ramprasad, T.; Krishna, K. S.; Basavaiah, N.

    2016-03-01

    We present high resolution paleosecular variation (PSV) records up to 8 cal. kyr BP from three piston cores, MD161/8, MD161/11 and MD161/13 acquired in the Krishna-Godavari (KG) basin, Bay of Bengal. During the Holocene period, high sedimentation rates are recorded at MD161/8 (38.8-248.3 cm/kyr), MD161/11 (137-336 cm/kyr) and MD161/13 (∼573 cm/kyr). Rock magnetic data analysis suggests that the remanence signal is carried by titanomagnetite/titanohematite grains in stable single domain (SSD)/pseudo single domain (PSD) state. The PSV records of MD161/11 and MD161/13 show good correlation in the uppermost sediments despite significant variation in the sedimentation rates; however, poor correlation of PSV records is observed for the core MD161/8 probably due to local effects. Paleoinclination records of MD161/8, MD161/11 and MD161/13 show a low between ∼2.4 and 2.0 cal. kyr BP, an increase between 2.0 and 1.4 cal. kyr BP and a decrease towards the present. To varying degrees these trends can be observed in the other Asian PSV records of Shuangchiling (SCL) and Biwa lakes. However, the magnitude of the observed inclination anomaly in KG basin is higher (∼40°) compared to those reported from SCL (∼25°) and Biwa (∼10°) lakes. Paleodeclination records of MD161/11 and MD161/13 show a decline between ∼4.0 and 2.9 cal. kyr BP, an increase between 2.9 and 2.1 cal. kyr BP, a substantial decrease between ∼2.1 and 1.0 cal. kyr BP and an increase towards the present. Similar trends can be observed in the other Asian PSV records of SCL and Biwa lakes with a minor age offset of 0.2-0.5 kyr. The available models CALS7k.2 and CALS10k.1 are evaluated for their capability in predicting the inclination and declination anomalies from the Asian regions. The CALS7k.2 model can predict most of the inclination anomalies while the CALS10k.1 is unable to predict many of them. The CALS7k.2 model shows that the observed inclination anomalies can be attributed to the presence of

  1. A New High-Resolution Record of the Blake Geomagnetic Excursion from ODP Site 1062

    NASA Astrophysics Data System (ADS)

    Bourne, M. D.; Mac Niocaill, C.; Henderson, G. M.; Thomas, A. L.; Faurschou Knudsen, M.

    2010-12-01

    We present a high resolution record of the Blake geomagnetic excursion from Ocean Drilling Program (ODP) Site 1062 on the Blake-Bahama Outer Ridge. The excursion is recorded in three separate cores, with the high sedimentation rate (~10 cm/ka) at this location allowing the determination of transitional field behaviour during the excursion. A complex geometry is observed for the excursional geomagnetic field at the site. The directional records show an initial deviation from the expected directions across an interval of 1 m that achieves a completely reversed state, and then returns to normal polarity. A second, although less well-defined, short-lived phase of anomalous directions is observed immediately following the first event in two of the three cores. Measurements of the magnetic susceptibility show little variation through the core indicating that the concentration and grain size of the remanence carriers remain relatively constant during the studied interval. Measurements of the S-Ratio and remanence coercivity also remain constant through the sections of interest, and indicate magnetite to be the primary remanence carrier. The relatively homogeneous sediment enables the determination of two relative palaeointensity proxies by normalizing natural remanent magnetization measurements using artificially induced magnetizations (anhysteretic remanence, ARM and isothermal remanence, IRM). These records are consistent between all three cores. The relative palaeointensity proxies suggest that the Earth's magnetic field decreased substantially in intensity several tens of kyr prior to the initial event, before reaching an intensity minimum coinciding with the directional excursion maximum. A second palaeointensity minimum is also observed after the excursional event with no associated directional change. These features are consistent with global palaeointensity stacks. Our age model uses a new oxygen isotope stratigraphy. However, rather than assuming a constant

  2. A high-resolution speleothem record of western equatorial Pacific rainfall: Implications for Holocene ENSO evolution

    NASA Astrophysics Data System (ADS)

    Chen, Sang; Hoffmann, Sharon S.; Lund, David C.; Cobb, Kim M.; Emile-Geay, Julien; Adkins, Jess F.

    2016-05-01

    coupled air-sea dynamics that are sensitive to precessional insolation forcing. Isolating the exact mechanisms that drive long-term ENSO evolution will require additional high-resolution paleoclimatic reconstructions and further investigation of Holocene tropical climate evolution using coupled climate models.

  3. A high resolution late Holocene palaeo environmental record from the central Adriatic Sea

    NASA Astrophysics Data System (ADS)

    Oldfield, F.; Asioli, A.; Accorsi, C. A.; Mercuri, A. M.; Juggins, S.; Langone, L.; Rolph, T.; Trincardi, F.; Wolff, G.; Gibbs, Z.; Vigliotti, L.; Frignani, M.; van der Post, K.; Branch, N.

    2003-02-01

    increased erosion, anti-ferromagetic minerals (haematite and/or goethite) become more important, as well as both paramagnetic minerals and super-paramagnetic magnetite. Analysis of the alkenone, U 37k', record provides an indication of possible changes in sea surface temperature during the period, but it is premature to place too much reliance on these inferred changes until the indirect effects of past changes in the depth of the halocline and in circulation have been more fully evaluated. The combination of methods used and the results obtained illustrate the potential value of such high resolution near-shore marine sedimentary sequences for recording wide-scale human impact, documenting the effects of this on marine sedimentation and fauna and, potentially, disentangling evidence for human activities from that for past changes in climate.

  4. Ship detection for high resolution optical imagery with adaptive target filter

    NASA Astrophysics Data System (ADS)

    Ju, Hongbin

    2015-10-01

    Ship detection is important due to both its civil and military use. In this paper, we propose a novel ship detection method, Adaptive Target Filter (ATF), for high resolution optical imagery. The proposed framework can be grouped into two stages, where in the first stage, a test image is densely divided into different detection windows and each window is transformed to a feature vector in its feature space. The Histograms of Oriented Gradients (HOG) is accumulated as a basic feature descriptor. In the second stage, the proposed ATF highlights all the ship regions and suppresses the undesired backgrounds adaptively. Each detection window is assigned a score, which represents the degree of the window belonging to a certain ship category. The ATF can be adaptively obtained by the weighted Logistic Regression (WLR) according to the distribution of backgrounds and targets of the input image. The main innovation of our method is that we only need to collect positive training samples to build the filter, while the negative training samples are adaptively generated by the input image. This is different to other classification method such as Support Vector Machine (SVM) and Logistic Regression (LR), which need to collect both positive and negative training samples. The experimental result on 1-m high resolution optical images shows the proposed method achieves a desired ship detection performance with higher quality and robustness than other methods, e.g., SVM and LR.

  5. High resolution in vivo intra-arterial imaging with optical coherence tomography

    PubMed Central

    Fujimoto, J; Boppart, S; Tearney, G; Bouma, B; Pitris, C; Brezinski, M

    1999-01-01

    BACKGROUND—Optical coherence tomography (OCT) is a new method of catheter based micron scale imaging. OCT is analogous to ultrasound, measuring the intensity of backreflected infrared light rather than sound waves.
OBJECTIVE—To demonstrate the ability of OCT to perform high resolution imaging of arterial tissue in vivo.
METHODS—OCT imaging of the abdominal aorta of New Zealand white rabbits was performed using a 2.9 F OCT imaging catheter. Using an ultrashort pulse laser as a light source for imaging, an axial resolution of 10 µm was achieved.
RESULTS—Imaging was performed at 4 frames/second and data were saved in either super VHS or digital format. Saline injections were required during imaging because of the signal attenuation caused by blood. Microstructure was sharply defined within the arterial wall and correlated with histology. Some motion artefacts were noted at 4 frames/second.
CONCLUSIONS—In vivo imaging of the rabbit aorta was demonstrated at a source resolution of 10 µm, but required the displacement of blood with saline. The high resolution of OCT allows imaging to be performed near the resolution of histopathology, offering the potential to have an impact both on the identification of high risk plaques and the guidance of interventional procedures.


Keywords: imaging; intravascular ultrasound; plaque rupture; optical coherence tomography PMID:10409522

  6. New optical sensor systems for high-resolution satellite, airborne and terrestrial imaging systems

    NASA Astrophysics Data System (ADS)

    Eckardt, Andreas; Börner, Anko; Lehmann, Frank

    2007-10-01

    The department of Optical Information Systems (OS) at the Institute of Robotics and Mechatronics of the German Aerospace Center (DLR) has more than 25 years experience with high-resolution imaging technology. The technology changes in the development of detectors, as well as the significant change of the manufacturing accuracy in combination with the engineering research define the next generation of spaceborne sensor systems focusing on Earth observation and remote sensing. The combination of large TDI lines, intelligent synchronization control, fast-readable sensors and new focal-plane concepts open the door to new remote-sensing instruments. This class of instruments is feasible for high-resolution sensor systems regarding geometry and radiometry and their data products like 3D virtual reality. Systemic approaches are essential for such designs of complex sensor systems for dedicated tasks. The system theory of the instrument inside a simulated environment is the beginning of the optimization process for the optical, mechanical and electrical designs. Single modules and the entire system have to be calibrated and verified. Suitable procedures must be defined on component, module and system level for the assembly test and verification process. This kind of development strategy allows the hardware-in-the-loop design. The paper gives an overview about the current activities at DLR in the field of innovative sensor systems for photogrammetric and remote sensing purposes.

  7. Experimental validation of a high-resolution diffuse optical imaging modality: photomagnetic imaging

    NASA Astrophysics Data System (ADS)

    Nouizi, Farouk; Luk, Alex; Thayer, Dave; Lin, Yuting; Ha, Seunghoon; Gulsen, Gultekin

    2016-01-01

    We present experimental results that validate our imaging technique termed photomagnetic imaging (PMI). PMI illuminates the medium under investigation with a near-infrared light and measures the induced temperature increase using magnetic resonance imaging. A multiphysics solver combining light and heat propagation is used to model spatiotemporal distribution of temperature increase. Furthermore, a dedicated PMI reconstruction algorithm has been developed to reveal high-resolution optical absorption maps from temperature measurements. Being able to perform measurements at any point within the medium, PMI overcomes the limitations of conventional diffuse optical imaging. We present experimental results obtained on agarose phantoms mimicking biological tissue with inclusions having either different sizes or absorption contrasts, located at various depths. The reconstructed images show that PMI can successfully resolve these inclusions with high resolution and recover their absorption coefficient with high-quantitative accuracy. Even a 1-mm inclusion located 6-mm deep is recovered successfully and its absorption coefficient is underestimated by only 32%. The improved PMI system presented here successfully operates under the maximum skin exposure limits defined by the American National Standards Institute, which opens up the exciting possibility of its future clinical use for diagnostic purposes.

  8. Experimental validation of a high-resolution diffuse optical imaging modality: photomagnetic imaging

    PubMed Central

    Nouizi, Farouk; Luk, Alex; Thayer, Dave; Lin, Yuting; Ha, Seunghoon; Gulsen, Gultekin

    2016-01-01

    Abstract. We present experimental results that validate our imaging technique termed photomagnetic imaging (PMI). PMI illuminates the medium under investigation with a near-infrared light and measures the induced temperature increase using magnetic resonance imaging. A multiphysics solver combining light and heat propagation is used to model spatiotemporal distribution of temperature increase. Furthermore, a dedicated PMI reconstruction algorithm has been developed to reveal high-resolution optical absorption maps from temperature measurements. Being able to perform measurements at any point within the medium, PMI overcomes the limitations of conventional diffuse optical imaging. We present experimental results obtained on agarose phantoms mimicking biological tissue with inclusions having either different sizes or absorption contrasts, located at various depths. The reconstructed images show that PMI can successfully resolve these inclusions with high resolution and recover their absorption coefficient with high-quantitative accuracy. Even a 1-mm inclusion located 6-mm deep is recovered successfully and its absorption coefficient is underestimated by only 32%. The improved PMI system presented here successfully operates under the maximum skin exposure limits defined by the American National Standards Institute, which opens up the exciting possibility of its future clinical use for diagnostic purposes. PMID:26790644

  9. High-resolution palaeomagnetic records of the Laschamp geomagnetic excursion from the Blake Ridge

    NASA Astrophysics Data System (ADS)

    Mac Niocaill, C.; Bourne, M. D.; Thomas, A. L.; Henderson, G. M.

    2013-05-01

    Geomagnetic excursions are brief (1000s of years) deviations in geomagnetic field behaviour from that expected during 'normal secular' variation. The Laschamp excursion (~41 ka) was a global deviation in geomagnetic field behaviour. Previously published records suggest rapid changes in field direction and a concurrent substantial decrease in field intensity. Accurate dating of excursions and determinations of their durations from multiple locations is vital to our understanding to global field behaviour during these deviations. We present here high-resolution palaeomagnetic records of the Laschamp excursion obtained from two Ocean Drilling Program (ODP) Sites 1061 and 1062 on the Blake-Bahama Outer Ridge (ODP Leg 172) Relatively high sedimentation rates (~30-40 cm kyr-1) at these locations allow the determination of transitional field behaviour during the excursion. Despite their advantages, sedimentary records can be limited by the potential for unrecognized variations in sedimentation rates between widely spaced age-constrained boundaries. Rather than assuming a constant sedimentation rate between assigned age tie-points, we employ measurements of the concentration of 230Thxs in the sediment. 230Thxs is a constant flux proxy and may be used to assess variations in the sedimentation rates through the core sections of interest. Following this approach, we present a new age model for Site 1061 that allows us to better determine the temporal behaviour of the Laschamp excursion with greater accuracy and known uncertainty. Palaeomagnetic measurements of discrete samples from four cores reveal a single excursional feature, across an interval of 30 cm, associated with a broader palaeointensity low. The excursion is characterised by rapid transitions (less than 200 years) between a stable normal polarity and a partially-reversed, polarity. Peaks in inclination either side of the directional excursion indicate periods of time when the local field is dominated by vertical

  10. An optical NMR spectrometer for Larmor-beat detection and high-resolution POWER NMR

    NASA Astrophysics Data System (ADS)

    Kempf, J. G.; Marohn, J. A.; Carson, P. J.; Shykind, D. A.; Hwang, J. Y.; Miller, M. A.; Weitekamp, D. P.

    2008-06-01

    Optical nuclear magnetic resonance (ONMR) is a powerful probe of electronic properties in III-V semiconductors. Larmor-beat detection (LBD) is a sensitivity optimized, time-domain NMR version of optical detection based on the Hanle effect. Combining LBD ONMR with the line-narrowing method of POWER (perturbations observed with enhanced resolution) NMR further enables atomically detailed views of local electronic features in III-Vs. POWER NMR spectra display the distribution of resonance shifts or line splittings introduced by a perturbation, such as optical excitation or application of an electric field, that is synchronized with a NMR multiple-pulse time-suspension sequence. Meanwhile, ONMR provides the requisite sensitivity and spatial selectivity to isolate local signals within macroscopic samples. Optical NMR, LBD, and the POWER method each introduce unique demands on instrumentation. Here, we detail the design and implementation of our system, including cryogenic, optical, and radio-frequency components. The result is a flexible, low-cost system with important applications in semiconductor electronics and spin physics. We also demonstrate the performance of our systems with high-resolution ONMR spectra of an epitaxial AlGaAs /GaAs heterojunction. NMR linewidths down to 4.1Hz full width at half maximum were obtained, a 103-fold resolution enhancement relative any previous optically detected NMR experiment.

  11. High-resolution temperature sensing in the Dead Sea using fiber optics

    NASA Astrophysics Data System (ADS)

    Arnon, A.; Lensky, N. G.; Selker, J. S.

    2014-02-01

    The thermal stratification of the Dead Sea was observed in high spatial and temporal resolution by means of fiber-optics temperature sensing. The aim of the research was to employ the novel high-resolution profiler in studying the dynamics of the thermal structure of the Dead Sea and the related processes including the investigation of the metalimnion fluctuations. The 18 cm resolution profiling system was placed vertically through the water column supported by a buoy 450 m from shore, from 2 m above to 53 m below the water surface (just above the local seafloor), covering the entire seasonal upper layer (the metalimnion had an average depth of ˜20 m). Temperature profiles were recorded every 5 min. The May to July 2012 data set allowed quantitative investigation of the thermal morphology dynamics, including objective definitions of key locations within the metalimnion based on the temperature depth profile and its first and second depth derivatives. Analysis of the fluctuation of the defined metalimnion locations showed strong anticorrelation to measured sea level fluctuations. The slope of the sea level versus metalimnion depth was found to be related to the density ratio of the upper layer and the underlying main water body, according to the prediction of a two-layer model. The heat content of the entire water column was calculated by integrating the temperature profiles. The vertically integrated apparent heat content was seen to vary by 50% in a few hours. These fluctuations were not correlated to the atmospheric heat fluxes, nor to the momentum transfer, but were highly correlated to the metalimnion and the sea level fluctuations (r = 0.84). The instantaneous apparent heat flux was 3 orders of magnitude larger than that delivered by radiation, with no direct correlation to the frequency of radiation and wind in the lake. This suggests that the source of the momentary heat flux is lateral advection due to internal waves (with no direct relation to the diurnal

  12. High-speed high-resolution optical coherence tomography at 800 and 1060 nm

    NASA Astrophysics Data System (ADS)

    Považay, B.; Hofer, B.; Hermann, B.; Torti, C.; Kajic, V.; Unterhuber, A.; Drexler, W.

    2008-09-01

    Two high speed systems for spectrometer based frequency domain optical coherence tomography are presented. A device operating at 800 nm, based on the Basler Sprint CMOS camera with linerates of up to 312,000 lps and a device based on the Goodrich SUI LHD 1024 px camera at 1060 nm with 47,000 lps are applied in a clinical environment to normal subjects. The feasibility of clinical high and ultra high-resolution optical coherence tomography (OCT) devices for retinal imaging at different wavelengths, capable of isotropic sampling with 70 to 600 frames per second at 512 depth scans/frame for widefield imaging and high density sampling at 1 Gvoxel are demostrated.

  13. Optical contrast enhancement of high-resolution ocular fundus imaging in vivo using polarimetry

    NASA Astrophysics Data System (ADS)

    Yang, Hansheng; Rao, Xuejun; Zhang, Yudong

    2007-11-01

    The adaptive optics (AO) retina imaging was performed with contrast enhancement by characterizing polarization parameters of the living retina. A removable pair of polarization state generating unit near the optical source and analysis unit near the CCD camera was incorporated into the basic 37-channle deformable mirror AO microscopic ophthalmoscope. Double-pass imaging polarimetry of the human eye was carried out, then incomplete Mueller matrix was calculated and analyzed to optimize the retina imaging condition using polarized light, which caused the subretinal structures with different polarization properties to emerge from the scattering light background, so the contrast of the image can be substantially enhanced. This method is demonstrated briefly and its validity was tested in the laboratory. The high-resolution images of ocular fundus are compared with 8-frame-averaging images we obtained prior to this method. The experiment results now show improved visualization of fundus structures to some extent without greatly sacrificing image resolution.

  14. High-resolution optical spectroscopy in a hollow-core photonic crystal fiber

    NASA Astrophysics Data System (ADS)

    Perrella, C.; Light, P. S.; Stace, T. M.; Benabid, F.; Luiten, A. N.

    2012-01-01

    In this paper, we present detailed high-resolution spectroscopy of rubidium (Rb) vapor confined within a hollow-core photonic crystal fiber (HC-PCF). We find a very low level of additional frequency broadening associated with this confinement, with spectral features being only 1 MHz broader than the natural linewidth of the excited state. We show that this additional broadening is consistent solely with the atoms' transit across the fiber's optical mode. This low level of decoherence opens the door to a wide variety of applications including compact frequency standards and new types of quantum optical devices based on alkali-metal-loaded HC-PCFs. We highlight the low level of decoherence through observation of electromagnetically induced transparency in the confined vapor.

  15. High-Resolution Optical Tweezers Combined With Single-Molecule Confocal Microscopy.

    PubMed

    Whitley, K D; Comstock, M J; Chemla, Y R

    2017-01-01

    We describe the design, construction, and application of an instrument combining dual-trap, high-resolution optical tweezers and a confocal microscope. This hybrid instrument allows nanomechanical manipulation and measurement simultaneously with single-molecule fluorescence detection. We present the general design principles that overcome the challenges of maximizing optical trap resolution while maintaining single-molecule fluorescence sensitivity, and provide details on the construction and alignment of the instrument. This powerful new tool is just beginning to be applied to biological problems. We present step-by-step instructions on an application of this technique that highlights the instrument's capabilities, detecting conformational dynamics in a nucleic acid-processing enzyme. © 2017 Elsevier Inc. All rights reserved.

  16. High-resolution fiber optic temperature sensors using nonlinear spectral curve fitting technique.

    PubMed

    Su, Z H; Gan, J; Yu, Q K; Zhang, Q H; Liu, Z H; Bao, J M

    2013-04-01

    A generic new data processing method is developed to accurately calculate the absolute optical path difference of a low-finesse Fabry-Perot cavity from its broadband interference fringes. The method combines Fast Fourier Transformation with nonlinear curve fitting of the entire spectrum. Modular functions of LabVIEW are employed for fast implementation of the data processing algorithm. The advantages of this technique are demonstrated through high performance fiber optic temperature sensors consisting of an infrared superluminescent diode and an infrared spectrometer. A high resolution of 0.01 °C is achieved over a large dynamic range from room temperature to 800 °C, limited only by the silica fiber used for the sensor.

  17. Application of Radar and Optical Images to Create Copernicus High Resolution Layers: Case Studies in Hungary

    NASA Astrophysics Data System (ADS)

    Surek, Gyorgy; Nador, Gizella; Friedl, Zoltan; Gyimesi, Balint; Rada, Matyas; Akos Gera, David; Hubik, Iren; Rotterne Kulesar, Aniko; Totok, Cecilia

    2016-08-01

    Injection of SAR imagery based information in the production of Copernicus High Resolution Layers can help to refine information served by optical satellite imagery, together with a-priori knowledge it may overcome the gaps caused by the cloud cover issue. However, this requires a methodological adaptation, given the different nature of SAR as compared to optical data. The methodological adaptation shall allow for an operational implementation, and shall help reducing the elapsed time between available satellite imagery. This requires the analysis of the potential use of SAR based imagery in the COPERNICUS land context, supported with case studies. In this paper the contribution of radar polarimetry for distinguishing land cover categories is evaluated.

  18. KiwiSpec - an advanced spectrograph for high resolution spectroscopy: optical design and variations

    NASA Astrophysics Data System (ADS)

    Barnes, Stuart I.; Gibson, Steve; Nield, Kathryn; Cochrane, Dave

    2012-09-01

    The KiwiSpec R4-100 is an advanced high resolution spectrograph developed by KiwiStar Optics, Industrial Research Ltd, New Zealand. The instrument is based around an R4 echelle grating and a 100mm collimated beam diameter. The optical design employs a highly asymmetric white pupil design, whereby the transfer collimator has a focal length only 1/3 that of the primary collimator. This allows the cross-dispersers (VPH gratings) and camera optics to be small and low cost while also ensuring a very compact instrument. The KiwiSpec instrument will be bre-fed and is designed to be contained in both thermal and/or vacuum enclosures. The instrument concept is highly exible in order to ensure that the same basic design can be used for a wide variety of science cases. Options include the possibility of splitting the wavelength coverage into 2 to 4 separate channels allowing each channel to be highly optimized for maximum eciency. CCDs ranging from smaller than 2K2K to larger than 4K4K can be accommodated. This allows good (3-4 pixel) sampling of resolving powers ranging from below 50,000 to greater than 100,000. Among the specic design options presented here will be a two-channel concept optimized for precision radial velocities, and a four-channel concept developed for the Gemini High- Resolution Optical Spectrograph (GHOST). The design and performance of a single-channel prototype will be presented elsewhere in these proceedings.

  19. High-resolution conodont oxygen isotope record of Ordovician climate change

    NASA Astrophysics Data System (ADS)

    Chen, J.; Chen, Z.; Algeo, T. J.

    2013-12-01

    The Ordovician Period was characterized by several major events, including a prolonged 'super greenhouse' during the Early Ordovician, the 'Great Ordovician Biodiversification Event (GOBE)' of the Middle and early Late Ordovician, and the Hirnantian ice age and mass extinction of the latest Ordovician (Webby et al., 2004, The Great Ordovician Biodiversification Event, Columbia University Press). The cause of the rapid diversification of marine invertebrates during the GOBE is not clear, however, and several scenarios have been proposed including widespread development of shallow cratonic seas, strong magmatic and tectonic activity, and climate moderation. In order to investigate relationships between climate change and marine ecosystem evolution during the Ordovician, we measured the oxygen isotopic composition of single coniform conodonts using a Cameca secondary ion mass spectrometer. Our δ18O profile shows a shift at the Early/Middle Ordovician transition that is indicative of a rapid 6 to 8 °C cooling. This cooling event marks the termination of the Early Ordovician 'super greenhouse' and may have established cooler tropical seawater temperatures that were more favorable for invertebrate animals, setting the stage for the GOBE. Additional cooling episodes occurred during the early Sandbian, early Katian, and Hirnantian, the last culminating in a short-lived (<1-Myr) end-Ordovician ice age. The much cooler conditions that prevailed at that time may have been an important factor in the end-Ordovician mass extinction. Our results differ from those of Trotter et al. (2008, 'Did cooling oceans trigger Ordovician biodiversification? Evidence from conodont thermometry,' Science 321:550-554). Instead of a slow, protracted cooling through the Early and Middle Ordovician, our high-resolution record shows that cooling occurred in several discrete steps, with the largest step being at the Early/Middle Ordovician transition.

  20. A high-resolution Late Quaternary upwelling record from the anoxic Cariaco Basin, Venezuela

    NASA Astrophysics Data System (ADS)

    Peterson, L. C.; Overpeck, J. T.; Kipp, N. G.; Imbrie, J.

    1991-02-01

    Results are presented of a high-resolution study of the planktonic foraminiferal faunas from two piston cores recovered from the Cariaco Basin in the southern Caribbean Sea. The Cariaco Basin is a small anoxic marine basin on the northern continental margin of Venezuela in an area today characterized by both seasonal trade wind-induced upwelling and pronounced dry and wet seasons. Our data indicate that large changes in the intensity of upwelling, and hence trade wind strength, occurred in this region during the last glacial-interglacial transition and throughout the Holocene. During the last glacial lowstand of sea level, the Cariaco Basin was effectively isolated from the open Caribbean along its northern margin by the then largely emergent Tortuga Bank. Oxic conditions existed in the deep Cariaco Basin at this time, and surface productivity was low. About 12,600 years ago, the abrupt initiation of strong upwelling over the basin and the onset of permanent anoxia in the deep waters are coincident with the rapid rise of sea level that accompanied the peak interval of meltwater discharge from the Laurentide Ice Sheet into the Gulf of Mexico. Strong upwelling between 12,600 and about 10,000 years ago may be related to intensified trade winds resulting, in part, from cooler sea surface temperatures in the Caribbean and Gulf of Mexico. After about 10,000 years ago, upwelling intensity was reduced, though highly variable. A preliminary frequency domain analysis of the Holocene portion of the Cariaco Basin time series suggests that solar forcing may explain a significant component of the century-scale variability observed in the record of upwelling and trade wind strength.

  1. A high-resolution air chemistry record from an Alpine ice core: Fiescherhorn glacier, Swiss Alps

    NASA Astrophysics Data System (ADS)

    Schwikowski, M.; Brütsch, S.; GäGgeler, H. W.; Schotterer, U.

    1999-06-01

    Glaciochemical studies at midlatitudes promise to contribute significantly to the understanding of the atmospheric cycling of species with short atmospheric lifetimes. Here we present results of chemical analyses of environmentally relevant species performed on an ice core from Fiescherhorn glacier, Swiss Alps (3890 m above sea level). This glacier site is unique since it is located near the high-alpine research station Jungfraujoch. There long-term meteorological and air quality measurements exist, which were used to calibrate the paleodata. The 77-m-long ice core was dated by annual layer counting using the seasonally varying signals of tritium and δ18O. It covers the time period 1946-1988 and shows a high net accumulation of water of 1.4 m yr-1 allowing for the reconstruction of high-resolution environmental records. Chemical composition was dominated by secondary aerosol constituents as well as mineral dust components, characterizing the Fiescherhorn site as a relatively unpolluted continental site. Concentrations of species like ammonium, nitrate, and sulfate showed an increasing trend from 1946 until about 1975, reflecting anthropogenic emission trends in western Europe. For mineral dust tracers, no trends were obvious, whereas chloride and sodium showed slightly higher levels from 1965 until 1988, indicating a change in the strength of sea-salt transport. Good agreement between the sulfate paleorecord with direct atmospheric measurements was found (correlation coefficient r2 = 0.41). Thus a "calibration" of the paleorecord over a significant period of time could be conducted, revealing an average scavenging ratio of 180 for sulfate.

  2. UDECON: deconvolution optimization software for restoring high-resolution records from pass-through paleomagnetic measurements

    NASA Astrophysics Data System (ADS)

    Xuan, Chuang; Oda, Hirokuni

    2015-11-01

    The rapid accumulation of continuous paleomagnetic and rock magnetic records acquired from pass-through measurements on superconducting rock magnetometers (SRM) has greatly contributed to our understanding of the paleomagnetic field and paleo-environment. Pass-through measurements are inevitably smoothed and altered by the convolution effect of SRM sensor response, and deconvolution is needed to restore high-resolution paleomagnetic and environmental signals. Although various deconvolution algorithms have been developed, the lack of easy-to-use software has hindered the practical application of deconvolution. Here, we present standalone graphical software UDECON as a convenient tool to perform optimized deconvolution for pass-through paleomagnetic measurements using the algorithm recently developed by Oda and Xuan (Geochem Geophys Geosyst 15:3907-3924, 2014). With the preparation of a format file, UDECON can directly read pass-through paleomagnetic measurement files collected at different laboratories. After the SRM sensor response is determined and loaded to the software, optimized deconvolution can be conducted using two different approaches (i.e., "Grid search" and "Simplex method") with adjustable initial values or ranges for smoothness, corrections of sample length, and shifts in measurement position. UDECON provides a suite of tools to view conveniently and check various types of original measurement and deconvolution data. Multiple steps of measurement and/or deconvolution data can be compared simultaneously to check the consistency and to guide further deconvolution optimization. Deconvolved data together with the loaded original measurement and SRM sensor response data can be saved and reloaded for further treatment in UDECON. Users can also export the optimized deconvolution data to a text file for analysis in other software.

  3. High-resolution in-depth imaging of optically cleared thick samples using an adaptive SPIM

    PubMed Central

    Masson, Aurore; Escande, Paul; Frongia, Céline; Clouvel, Grégory; Ducommun, Bernard; Lorenzo, Corinne

    2015-01-01

    Today, Light Sheet Fluorescence Microscopy (LSFM) makes it possible to image fluorescent samples through depths of several hundreds of microns. However, LSFM also suffers from scattering, absorption and optical aberrations. Spatial variations in the refractive index inside the samples cause major changes to the light path resulting in loss of signal and contrast in the deepest regions, thus impairing in-depth imaging capability. These effects are particularly marked when inhomogeneous, complex biological samples are under study. Recently, chemical treatments have been developed to render a sample transparent by homogenizing its refractive index (RI), consequently enabling a reduction of scattering phenomena and a simplification of optical aberration patterns. One drawback of these methods is that the resulting RI of cleared samples does not match the working RI medium generally used for LSFM lenses. This RI mismatch leads to the presence of low-order aberrations and therefore to a significant degradation of image quality. In this paper, we introduce an original optical-chemical combined method based on an adaptive SPIM and a water-based clearing protocol enabling compensation for aberrations arising from RI mismatches induced by optical clearing methods and acquisition of high-resolution in-depth images of optically cleared complex thick samples such as Multi-Cellular Tumour Spheroids. PMID:26576666

  4. High resolution record of the Last Glacial Maximum in eastern Australia

    NASA Astrophysics Data System (ADS)

    Petherick, Lynda; Moss, Patrick; McGowan, Hamish

    2010-05-01

    A continuous, high resolution (average ca. 22 year) record encompassing the Last Glacial Maximum (LGM) has been developed using multiple proxies (aeolian sediment flux, grain size, pollen and charcoal) in lake sediment from Tortoise Lagoon (TOR), North Stradbroke Island, Queensland, Australia. The presence of Asteraceae tubilifloreae and spineless Asteraceae (common indicators of glacial conditions in Australia) at TOR indicates significantly cooler temperatures (mean annual temperature up to 6oC lower than today). In addition to the palaeoclimatic reconstruction, a record of palaeodust transport pathways for eastern Australia was developed using ICP-MS trace element analysis and geochemical "fingerprinting" of TOR aeolian sediment to continental dust source areas. Vectors between dominant dust source areas and North Stradbroke Island allowed the reconstruction of the position and intensity of LGM dust transport pathways. Furthermore, changes in likely synpotic scale conditions can be postulated based on the position of the dust transport corridors. Similarities between the vegetation at TOR during the LGM and that at temperate sites e.g. Caledonia Fen, Victoria (Kershaw et al. 2007), Redhead Lagoon, New South Wales (Williams et al. 2006) and Barrington Tops, New South Wales (Sweller and Martin 2001) suggests that this record reflects regional conditions across southeastern Australia. The TOR record also correlates well with that from nearby Native Companion Lagoon which suggests that the LGM was actually an extended period of ca. 8 - 10 kyr, characterised by 2 periods of increased aridity (ca. 30 - 26.5 kyr and 21 - 19.5 kyr) (Petherick et al. 2008). A growing number of records from across the Southern Hemisphere e.g. New Zealand (Suggate and Almond 2003; Alloway et al. 2007; Newnham et al. 2007), Chile (Denton et al. 1999), Antarctica (Röthlisberger et al. 2002; EPICA 2006) and Australia (Smith 2009) also show evidence that the LGM encompassed a longer period of

  5. Repeated, noninvasive, high resolution spectral domain optical coherence tomography imaging of zebrafish embryos

    PubMed Central

    Kagemann, Larry; Ishikawa, Hiroshi; Zou, Jian; Charukamnoetkanok, Puwat; Wollstein, Gadi; Townsend, Kelly A.; Gabriele, Michelle L.; Bahary, Nathan; Wei, Xiangyun; Fujimoto, James G.

    2008-01-01

    Purpose To demonstrate a new imaging method for high resolution spectral domain optical coherence tomography (SD-OCT) for small animal developmental imaging. Methods Wildtype zebrafish that were 24, 48, 72, and 120 h post fertilization (hpf) and nok gene mutant (48 hpf) embryos were imaged in vivo. Three additional embryos were imaged twice, once at 72 hpf and again at 120 hpf. Images of the developing eye, brain, heart, whole body, proximal yolk sac, distal yolk sac, and tail were acquired. Three-dimensional OCT data sets (501×180 axial scans) were obtained as well as oversampled frames (8,100 axial scans) and repeated line scans (180 repeated frames). Scan volumes ranged from 750×750 µm to 3×3 mm, each 1.8 mm thick. Three-dimenstional data sets allowed construction of C-mode slabs of the embryo. Results SD-OCT provided ultra-high resolution visualization of the eye, brain, heart, ear, and spine of the developing embryo as early as 24 hpf, and allowed development to be documented in each of these organ systems in consecutive sessions. Repeated line scanning with averaging optimized the visualization of static and dynamic structures contained in SD-OCT images. Structural defects caused by a mutation in the nok gene were readily observed as impeded ocular development, and enlarged pericardial cavities. Conclusions SD-OCT allowed noninvasive, in vivo, ultra-high resolution, high-speed imaging of zebrafish embryos in their native state. The ability to measure structural and functional features repeatedly on the same specimen, without the need to sacrifice, promises to be a powerful tool in small animal developmental imaging. PMID:19052656

  6. Micrometer scale contact lens movements imaged by ultra-high resolution optical coherence tomography

    PubMed Central

    Cui, Lele; Shen, Meixiao; Wang, Michael R.; Wang, Jianhua

    2011-01-01

    PURPOSE To dynamically evaluate contact lens movement and ocular surface shape using ultra-high resolution and ultra-long scan depth optical coherence tomography (OCT). DESIGN Clinical research study of a laboratory technique. METHODS Four different types of soft contact lenses were tested on the left eye of 10 subjects (6 males and 4 females). Lenses edges at primary gaze and temporal and nasal gazes were imaged by ultra-high resolution OCT. Excursion lag was obtained as the distance between the lens edge at primary gaze and immediately after the eye was quickly turned either nasally or temporally. The inferior lens edges were imaged continuously to track vertical movements during blinking. Ultra-long scan depth OCT provided quantifiable images of the ocular surface, and the contour was acquired using custom software. RESULTS Excursion lag at the horizontal meridian was 366 ± 134 μm at temporal gaze and 320 ± 137 μm at nasal gaze (P > .05). The lens uplift at the vertical meridian was 342 ± 155 μm after blinking. There were significant differences in horizontal lags and vertical movements among different lenses (P < .05). Horizontal lags were correlated with radii of curvatures and sagittal heights at 6- and 14- mm horizontal meridian radii (P < .05). The blink-induced lens uplift first lowered by 104 ± 8 μm, and then lifted 342 ± 155 μm after the blink. CONCLUSIONS Ultra-high resolution and ultra-long scan depth OCT can assess micrometer scale lens movements and ocular surface contours. Both lens design and ocular surface shape affected lens movements. PMID:21920493

  7. High resolution windows into early Holocene climate: Sr/(Ca) coral records from the Huon Peninsula

    NASA Astrophysics Data System (ADS)

    McCulloch, Malcolm; Mortimer, Graham; Esat, Tezer; Xianhua, Li; Pillans, Brad; Chappell, John

    1996-02-01

    High-precision measurements of Sr/Ca ratios are reported for Porites corals from the uplifted Holocene coral terraces at Huon Peninsula, Papua New Guinea. The early Holocene Porites have UTh mass spectrometric ages of 8920 ± 60 yr and 7370 ± 50 yr, and δ 234U(t) values of 145 ± 2, similar to modern seawater. The Sr/Ca coral records provide 5-6 year high resolution (near weekly) time windows into early Holocene sea surface temperatures. Seasonal temperature fluctuations are generally in the range of ± 1°C, with occasional excursions of ± 2°C, which may indicate the more frequent recurrence of very strong ENSO (El Niño-Southern Oscillation) events. Mean annual Sr/Ca temperatures of 24.2 ± 1.1°C and 22.9 ± 0.8°C have been obtained, which are ˜ 2-3°C cooler than that exhibited by a modern Porites. These results indicate that, during the early Holocene, the equatorial western Pacific ocean was at least several degrees cooler than present-day temperatures. This is consistent with late glacial coral records from the Caribbean that indicate lower (˜ 6°C) sea surface temperatures for the equatorial oceans. The Huon Peninsula corals also indicate that SSTs were several degrees cooler than those in the Caribbean during the early Holocene. Thus, although the northern hemisphere summer radiation maximum occurred at ˜ 10 ka, there appears to have been a significant lag in the response of the equatorial western Pacific ocean to this warming. Cooler early Holocene sea surface temperatures in the western Pacific may have been due to changing patterns of ocean-atmosphere circulation, resulting from the exposure of large areas of continental shelf in the southeast Asia region, a consequence of lower glacial sea levels. It is likely that ocean temperatures in the Huon Peninsula were influenced by the opening at ˜ 7 ka of the Torres Strait, that now separates New Guinea from the Australian mainland.

  8. Development of a flexible optical fiber based high resolution integrated PET∕MRI system.

    PubMed

    Yamamoto, Seiichi; Watabe, Hiroshi; Kanai, Yasukazu; Watabe, Tadashi; Aoki, Masaaki; Sugiyama, Eiji; Kato, Katsuhiko; Hatazawa, Jun

    2012-11-01

    The simultaneous measurement of PET and magnetic resonance imaging (MRI) is an emerging field for molecular imaging research. Although optical fiber based PET∕MRI systems have advantages on less interference between PET and MRI, there is a drawback in reducing the scintillation light due to the fiber. To reduce the problem, the authors newly developed flexible optical fiber bundle based block detectors and employed them for a high resolution integrated PET∕MRI system. The flexible optical fiber bundle used 0.5 mm diameter, 80 cm long double clad fibers which have dual 12 mm × 24 mm rectangular inputs and a single 24 mm × 24 mm rectangular output. In the input surface, LGSO scintillators of 0.025 mol.% (decay time: ∼31 ns: 0.9 mm × 1.3 mm × 5 mm) and 0.75 mol.% (decay time: ∼46 ns: 0.9 mm × 1.3 mm × 6 mm) were optically coupled in depth direction to form depth-of-interaction detector, arranged in 11 × 13 matrix and optically coupled to the fiber bundle. The two inputs of the bundle are bent for 90°, bound to one, and are optically coupled to a Hamamatsu 1-in. square position sensitive photomultiplier tube. Light loss due to the fiber bundle could be reduced and the performance of the block detectors was improved. Eight optical fiber based block detectors (16 LGSO blocks) were arranged in a 56 mm diameter ring to form a PET system. Spatial resolution and sensitivity were 1.2 mm full-width at half-maximum and 1.2% at the central field-of-view, respectively. Sensitivity change was less than 1% for 2 °C temperature changes. This PET system was integrated with a 0.3 T permanent magnet MRI system which has 17 cm diameter hole at the yoke area for insertion of the PET detector ring. There was no observable interference between PET and MRI. Simultaneous imaging of PET and MRI was successfully performed for small animal studies. The authors confirmed that the developed high resolution PET∕MRI system is promising for molecular

  9. Development of a flexible optical fiber based high resolution integrated PET/MRI system

    SciTech Connect

    Yamamoto, Seiichi; Watabe, Hiroshi; Kanai, Yasukazu; Watabe, Tadashi; Aoki, Masaaki; Sugiyama, Eiji; Kato, Katsuhiko; Hatazawa, Jun

    2012-11-15

    Purpose: The simultaneous measurement of PET and magnetic resonance imaging (MRI) is an emerging field for molecular imaging research. Although optical fiber based PET/MRI systems have advantages on less interference between PET and MRI, there is a drawback in reducing the scintillation light due to the fiber. To reduce the problem, the authors newly developed flexible optical fiber bundle based block detectors and employed them for a high resolution integrated PET/MRI system. Methods: The flexible optical fiber bundle used 0.5 mm diameter, 80 cm long double clad fibers which have dual 12 mm Multiplication-Sign 24 mm rectangular inputs and a single 24 mm Multiplication-Sign 24 mm rectangular output. In the input surface, LGSO scintillators of 0.025 mol.% (decay time: {approx}31 ns: 0.9 mm Multiplication-Sign 1.3 mm Multiplication-Sign 5 mm) and 0.75 mol.% (decay time: {approx}46 ns: 0.9 mm Multiplication-Sign 1.3 mm Multiplication-Sign 6 mm) were optically coupled in depth direction to form depth-of-interaction detector, arranged in 11 Multiplication-Sign 13 matrix and optically coupled to the fiber bundle. The two inputs of the bundle are bent for 90 Degree-Sign , bound to one, and are optically coupled to a Hamamatsu 1-in. square position sensitive photomultiplier tube. Results: Light loss due to the fiber bundle could be reduced and the performance of the block detectors was improved. Eight optical fiber based block detectors (16 LGSO blocks) were arranged in a 56 mm diameter ring to form a PET system. Spatial resolution and sensitivity were 1.2 mm full-width at half-maximum and 1.2% at the central field-of-view, respectively. Sensitivity change was less than 1% for 2 Degree-Sign C temperature changes. This PET system was integrated with a 0.3 T permanent magnet MRI system which has 17 cm diameter hole at the yoke area for insertion of the PET detector ring. There was no observable interference between PET and MRI. Simultaneous imaging of PET and MRI was

  10. Multimodal adaptive optics for depth-enhanced high-resolution ophthalmic imaging

    NASA Astrophysics Data System (ADS)

    Hammer, Daniel X.; Mujat, Mircea; Iftimia, Nicusor V.; Lue, Niyom; Ferguson, R. Daniel

    2010-02-01

    We developed a multimodal adaptive optics (AO) retinal imager for diagnosis of retinal diseases, including glaucoma, diabetic retinopathy (DR), age-related macular degeneration (AMD), and retinitis pigmentosa (RP). The development represents the first ever high performance AO system constructed that combines AO-corrected scanning laser ophthalmoscopy (SLO) and swept source Fourier domain optical coherence tomography (SSOCT) imaging modes in a single compact clinical prototype platform. The SSOCT channel operates at a wavelength of 1 μm for increased penetration and visualization of the choriocapillaris and choroid, sites of major disease activity for DR and wet AMD. The system is designed to operate on a broad clinical population with a dual deformable mirror (DM) configuration that allows simultaneous low- and high-order aberration correction. The system also includes a wide field line scanning ophthalmoscope (LSO) for initial screening, target identification, and global orientation; an integrated retinal tracker (RT) to stabilize the SLO, OCT, and LSO imaging fields in the presence of rotational eye motion; and a high-resolution LCD-based fixation target for presentation to the subject of stimuli and other visual cues. The system was tested in a limited number of human subjects without retinal disease for performance optimization and validation. The system was able to resolve and quantify cone photoreceptors across the macula to within ~0.5 deg (~100-150 μm) of the fovea, image and delineate ten retinal layers, and penetrate to resolve targets deep into the choroid. In addition to instrument hardware development, analysis algorithms were developed for efficient information extraction from clinical imaging sessions, with functionality including automated image registration, photoreceptor counting, strip and montage stitching, and segmentation. The system provides clinicians and researchers with high-resolution, high performance adaptive optics imaging to help

  11. High-resolution adaptive optics retinal imaging of cellular structure in choroideremia.

    PubMed

    Morgan, Jessica I W; Han, Grace; Klinman, Eva; Maguire, William M; Chung, Daniel C; Maguire, Albert M; Bennett, Jean

    2014-09-04

    We characterized retinal structure in patients and carriers of choroideremia using adaptive optics and other high resolution modalities. A total of 57 patients and 18 carriers of choroideremia were imaged using adaptive optics scanning light ophthalmoscopy (AOSLO), optical coherence tomography (OCT), autofluorescence (AF), and scanning light ophthalmoscopy (SLO). Cone density was measured in 59 eyes of 34 patients where the full cone mosaic was observed. The SLO imaging revealed scalloped edges of RPE atrophy and large choroidal vessels. The AF imaging showed hypo-AF in areas of degeneration, while central AF remained present. OCT images showed outer retinal tubulations and thinned RPE/interdigitation layers. The AOSLO imaging revealed the cone mosaic in central relatively intact retina, and cone density was either reduced or normal at 0.5 mm eccentricity. The border of RPE atrophy showed abrupt loss of the cone mosaic at the same location. The AF imaging in comparison with AOSLO showed RPE health may be compromised before cone degeneration. Other disease features, including visualization of choroidal vessels, hyper-reflective clumps of cones, and unique retinal findings, were tabulated to show the frequency of occurrence and model disease progression. The data support the RPE being one primary site of degeneration in patients with choroideremia. Photoreceptors also may degenerate independently. High resolution imaging, particularly AOSLO in combination with OCT, allows single cell analysis of disease in choroideremia. These modalities promise to be useful in monitoring disease progression, and in documenting the efficacy of gene and cell-based therapies for choroideremia and other diseases as these therapies emerge. (ClinicalTrials.gov number, NCT01866371.). Copyright 2014 The Association for Research in Vision and Ophthalmology, Inc.

  12. High-resolution imaging of the retinal nerve fiber layer in normal eyes using adaptive optics scanning laser ophthalmoscopy.

    PubMed

    Takayama, Kohei; Ooto, Sotaro; Hangai, Masanori; Arakawa, Naoko; Oshima, Susumu; Shibata, Naohisa; Hanebuchi, Masaaki; Inoue, Takashi; Yoshimura, Nagahisa

    2012-01-01

    To conduct high-resolution imaging of the retinal nerve fiber layer (RNFL) in normal eyes using adaptive optics scanning laser ophthalmoscopy (AO-SLO). AO-SLO images were obtained in 20 normal eyes at multiple locations in the posterior polar area and a circular path with a 3-4-mm diameter around the optic disc. For each eye, images focused on the RNFL were recorded and a montage of AO-SLO images was created. AO-SLO images for all eyes showed many hyperreflective bundles in the RNFL. Hyperreflective bundles above or below the fovea were seen in an arch from the temporal periphery on either side of a horizontal dividing line to the optic disc. The dark lines among the hyperreflective bundles were narrower around the optic disc compared with those in the temporal raphe. The hyperreflective bundles corresponded with the direction of the striations on SLO red-free images. The resolution and contrast of the bundles were much higher in AO-SLO images than in red-free fundus photography or SLO red-free images. The mean hyperreflective bundle width around the optic disc had a double-humped shape; the bundles at the temporal and nasal sides of the optic disc were narrower than those above and below the optic disc (P<0.001). RNFL thickness obtained by optical coherence tomography correlated with the hyperreflective bundle widths on AO-SLO (P<0.001) AO-SLO revealed hyperreflective bundles and dark lines in the RNFL, believed to be retinal nerve fiber bundles and Müller cell septa. The widths of the nerve fiber bundles appear to be proportional to the RNFL thickness at equivalent distances from the optic disc.

  13. High-resolution, large dynamic range fiber-optic thermometer with cascaded Fabry-Perot cavities.

    PubMed

    Liu, Guigen; Sheng, Qiwen; Hou, Weilin; Han, Ming

    2016-11-01

    The paradox between a large dynamic range and a high resolution commonly exists in nearly all kinds of sensors. Here, we propose a fiber-optic thermometer based on dual Fabry-Perot interferometers (FPIs) made from the same material (silicon), but with different cavity lengths, which enables unambiguous recognition of the dense fringes associated with the thick FPI over the free-spectral range determined by the thin FPI. Therefore, the sensor combines the large dynamic range of the thin FPI and the high resolution of the thick FPI. To verify this new concept, a sensor with one 200 μm thick silicon FPI cascaded by another 10 μm thick silicon FPI was fabricated. A temperature range of -50°C to 130°C and a resolution of 6.8×10-3°C were demonstrated using a simple average wavelength tracking demodulation. Compared to a sensor with only the thick silicon FPI, the dynamic range of the hybrid sensor was more than 10 times larger. Compared to a sensor with only the thin silicon FPI, the resolution of the hybrid sensor was more than 18 times higher.

  14. Loki: a ground-layer adaptive optics high-resolution near-infrared survey camera

    NASA Astrophysics Data System (ADS)

    Baranec, Christoph; Lloyd-Hart, Michael; Meyer, Michael

    2007-09-01

    We present the design of a new high-resolution near-infrared survey camera that will take advantage of the wide corrected field afforded by the 6.5 m MMT's new multi-laser ground-layer adaptive optics (GLAO) system. GLAO technology will correct for turbulence close to the telescope aperture where typically 1/2 to 2/3 of the total atmospheric turbulence lies and is expected to deliver image widths of 0.1-0.2 arc seconds in the near-infrared across a wide range of seeing conditions. The new camera will use a 2 by 2 mosaic of JWST NIRCam detectors, 2048 x 2048 arrays sensitive from 0.6 - 2.5 μm based on Teledyne's HgCdTe HAWAII-2RG detector technology. The camera has a 4 arc minute square field, giving a plate scale of approximately 0.06 arc seconds/pixel, critically sampling the GLAO PSF. In addition, high resolution (0.25 arc seconds or better) multi-object spectroscopy can be supported with cold slit masks inside the dewar; allowing potentially hundreds of spectra to be obtained at once with resolutions of up to 10,000.

  15. High resolution in vivo intra-arterial imaging with optical coherence tomography.

    PubMed

    Fujimoto, J G; Boppart, S A; Tearney, G J; Bouma, B E; Pitris, C; Brezinski, M E

    1999-08-01

    Optical coherence tomography (OCT) is a new method of catheter based micron scale imaging. OCT is analogous to ultrasound, measuring the intensity of backreflected infrared light rather than sound waves. To demonstrate the ability of OCT to perform high resolution imaging of arterial tissue in vivo. OCT imaging of the abdominal aorta of New Zealand white rabbits was performed using a 2.9 F OCT imaging catheter. Using an ultrashort pulse laser as a light source for imaging, an axial resolution of 10 micrometer was achieved. Imaging was performed at 4 frames/second and data were saved in either super VHS or digital format. Saline injections were required during imaging because of the signal attenuation caused by blood. Microstructure was sharply defined within the arterial wall and correlated with histology. Some motion artefacts were noted at 4 frames/second. In vivo imaging of the rabbit aorta was demonstrated at a source resolution of 10 micrometer, but required the displacement of blood with saline. The high resolution of OCT allows imaging to be performed near the resolution of histopathology, offering the potential to have an impact both on the identification of high risk plaques and the guidance of interventional procedures.

  16. A High-resolution Speleothem Record of Western Equatorial Pacific Rainfall: Implications for Holocene ENSO Evolution

    NASA Astrophysics Data System (ADS)

    Chen, S.; Hoffmann, S. S.; Lund, D. C.

    2013-12-01

    The El Niño-Southern Oscillation (ENSO) is the primary driver of interannual climate variability in the tropics and subtropics. Despite substantial progress in understanding ocean-atmosphere feedbacks that drive ENSO today, relatively little is known about its behavior on centennial and longer timescales. Paleoclimate records from lakes, corals and deep-sea sediments suggest ENSO variability was generally weaker during the mid-Holocene (4-6 kyr BP) than the late Holocene (0-4 kyr BP) [1-3]. However, discrepancies amongst the records preclude a clear timeline of Holocene ENSO evolution and therefore attribution of ENSO variability to a specific climate forcing. Here we present δ18O results from a U-Th dated speleothem in Malaysian Borneo nominally sampled at seasonal resolution. The δ18O of Borneo rainfall is a robust proxy of regional convective intensity and precipitation amount, both of which are directly influenced by ENSO activity [4-5]. Replicated δ18O records from multiple cave locations in Borneo indicate stalagmites from the region represent regionally coherent changes in atmospheric convection [6-7]. Our estimates of stalagmite δ18O variance at ENSO periods (2-7 years) show a significant (p < 0.01) reduction in interannual variability during the mid-Holocene (4580-4760 yr BP) relative to both the late Holocene (2400-2600 yr BP) and early Holocene (6620-6720 yr BP and 8100-8300 yr BP). The Borneo results are generally consistent with Holocene records of interannual variability from the eastern equatorial Pacific [2-3], indicating that long-term changes in ENSO were likely the primary driver. Reduced mid-Holocene δ18O variability in Borneo coincides with an overall minimum in δ18O from 3.5 to 5.5 kyr BP, suggesting the low-frequency signal is driven by persistent atmospheric convection in the Western Pacific Warm Pool (WPWP). Additional high resolution time slices are necessary to fully characterize interannual-scale δ18O variance in Borneo and to

  17. Reference-free, high-resolution measurement method of timing jitter spectra of optical frequency combs

    PubMed Central

    Kwon, Dohyeon; Jeon, Chan-Gi; Shin, Junho; Heo, Myoung-Sun; Park, Sang Eon; Song, Youjian; Kim, Jungwon

    2017-01-01

    Timing jitter is one of the most important properties of femtosecond mode-locked lasers and optical frequency combs. Accurate measurement of timing jitter power spectral density (PSD) is a critical prerequisite for optimizing overall noise performance and further advancing comb applications both in the time and frequency domains. Commonly used jitter measurement methods require a reference mode-locked laser with timing jitter similar to or lower than that of the laser-under-test, which is a demanding requirement for many laser laboratories, and/or have limited measurement resolution. Here we show a high-resolution and reference-source-free measurement method of timing jitter spectra of optical frequency combs using an optical fibre delay line and optical carrier interference. The demonstrated method works well for both mode-locked oscillators and supercontinua, with 2 × 10−9 fs2/Hz (equivalent to −174 dBc/Hz at 10-GHz carrier frequency) measurement noise floor. The demonstrated method can serve as a simple and powerful characterization tool for timing jitter PSDs of various comb sources including mode-locked oscillators, supercontinua and recently emerging Kerr-frequency combs; the jitter measurement results enabled by our method will provide new insights for understanding and optimizing timing noise in such comb sources. PMID:28102352

  18. Reference-free, high-resolution measurement method of timing jitter spectra of optical frequency combs

    NASA Astrophysics Data System (ADS)

    Kwon, Dohyeon; Jeon, Chan-Gi; Shin, Junho; Heo, Myoung-Sun; Park, Sang Eon; Song, Youjian; Kim, Jungwon

    2017-01-01

    Timing jitter is one of the most important properties of femtosecond mode-locked lasers and optical frequency combs. Accurate measurement of timing jitter power spectral density (PSD) is a critical prerequisite for optimizing overall noise performance and further advancing comb applications both in the time and frequency domains. Commonly used jitter measurement methods require a reference mode-locked laser with timing jitter similar to or lower than that of the laser-under-test, which is a demanding requirement for many laser laboratories, and/or have limited measurement resolution. Here we show a high-resolution and reference-source-free measurement method of timing jitter spectra of optical frequency combs using an optical fibre delay line and optical carrier interference. The demonstrated method works well for both mode-locked oscillators and supercontinua, with 2 × 10‑9 fs2/Hz (equivalent to ‑174 dBc/Hz at 10-GHz carrier frequency) measurement noise floor. The demonstrated method can serve as a simple and powerful characterization tool for timing jitter PSDs of various comb sources including mode-locked oscillators, supercontinua and recently emerging Kerr-frequency combs; the jitter measurement results enabled by our method will provide new insights for understanding and optimizing timing noise in such comb sources.

  19. Diamond x-ray optics: Transparent, resilient, high-resolution, and wavefront preserving

    DOE PAGES

    Shvyd’ko, Yuri; Blank, Vladimir; Terentyev, Sergey

    2017-06-09

    Diamond features a unique combination of outstanding physical properties perfect for numerous x-ray optics applications, where traditional materials such as silicon fail to perform. In the last two decades, impressive progress has been achieved in synthesizing diamond with high crystalline perfection, in manufacturing efficient, resilient, high-resolution, wavefront-preserving diamond optical components, and in implementing them in cutting-edge x-ray instruments. Diamond optics are essential for tailoring x-rays to the most challenging needs of x-ray research. Furthermore, they are becoming vital for the generation of fully coherent hard x-rays by seeded x-ray free-electron lasers. In this article, we review progress in manufacturing flawlessmore » diamond crystal components and their applications in diverse x-ray optical devices, such as x-ray monochromators, beam splitters, high-reflectance backscattering mirrors, lenses, phase plates, diffraction gratings, bent-crystal spectrographs, and windows.« less

  20. High resolution optical shaft encoder for motor speed control based on an optical disk pick-up

    NASA Astrophysics Data System (ADS)

    Yeh, Wei-Hung; Bletscher, Warren; Mansuripur, M.

    1998-08-01

    Using a three-beam optical pick-up from a compact disk player and a flexible, shaft-mounted diffraction grating, we obtain information about the rotation speed and angular position of the motor's spindle. This information may be used for feedback to the motor for smooth operation. Due to the small size of the focused spot and the built-in auto-focus mechanism of the optical head, the proposed encoder can achieve submicrometer resolution. With high resolution, reliable operation, and low-cost elements, the proposed method is suitable for rotary and linear motion control where accurate positioning of an object is required.

  1. Simultaneous high-resolution pH and spectrophotometric recordings of oxygen binding in blood microvolumes.

    PubMed

    Oellermann, Michael; Pörtner, Hans-O; Mark, Felix C

    2014-05-01

    Oxygen equilibrium curves have been widely used to understand oxygen transport in numerous organisms. A major challenge has been to monitor oxygen binding characteristics and concomitant pH changes as they occur in vivo, in limited sample volumes. Here we report a technique allowing highly resolved and simultaneous monitoring of pH and blood pigment saturation in minute blood volumes. We equipped a gas diffusion chamber with a broad-range fibre-optic spectrophotometer and a micro-pH optode and recorded changes of pigment oxygenation along oxygen partial pressure (PO2) and pH gradients to test the setup. Oxygen binding parameters derived from measurements in only 15 μl of haemolymph from the cephalopod Octopus vulgaris showed low instrumental error (0.93%) and good agreement with published data. Broad-range spectra, each resolving 2048 data points, provided detailed insight into the complex absorbance characteristics of diverse blood types. After consideration of photobleaching and intrinsic fluorescence, pH optodes yielded accurate recordings and resolved a sigmoidal shift of 0.03 pH units in response to changing PO2 from 0 to 21 kPa. Highly resolved continuous recordings along pH gradients conformed to stepwise measurements at low rates of pH changes. In this study we showed that a diffusion chamber upgraded with a broad-range spectrophotometer and an optical pH sensor accurately characterizes oxygen binding with minimal sample consumption and manipulation. We conclude that the modified diffusion chamber is highly suitable for experimental biologists who demand high flexibility, detailed insight into oxygen binding as well as experimental and biological accuracy combined in a single setup.

  2. Tissue differentiation by means of high resolution optical emission spectroscopy during electrosurgical intervention

    NASA Astrophysics Data System (ADS)

    Bürger, Ines; Scharpf, Marcus; Hennenlotter, Jörg; Nüßle, Daniela; Spether, Dominik; Neugebauer, Alexander; Bibinov, Nikita; Stenzl, Arnulf; Fend, Falko; Enderle, Markus; Awakowicz, Peter

    2017-01-01

    Electrosurgery is the use of radio-frequency electric current for the cutting of biological tissue e.g. for resection of tumour tissue. In this work, the optical emission of plasma being generated during the electrosurgical procedure is investigated with a high resolution echelle spectrometer to find differences between tumour tissue and normal renal tissue in a pre-clinical ex vivo study. Trace elements like zinc, iron, copper and cadmium are present in the tissue spectra as well as the electrolytes magnesium, calcium, sodium and potassium and some diatomic molecules such as hydroxyl radical, cyano radical, dicarbon, nitrogen monohydride and molecular nitrogen which are mainly dissociated from polyatomic molecules. With the atomic emission line of cadmium at 228.8 nm the treated tissue can be differentiated in tumorous and healthy tissue with correct assignment of 95% for tumour tissue and 92% for normal renal tissue.

  3. High resolution spectral domain optical coherence tomography (OCT) images of Alström Syndrome

    PubMed Central

    Vingolo, EM; Salvatore, S.; Grenga, PL; Maffei, P.; Milan, G.; Marshall, JD

    2010-01-01

    Alström syndrome (ALMS1) is a multisystemic disorder characterized by cone–rod dystrophy, hearing loss, obesity, insulin resistance and hyperinsulinemia, type 2 diabetes mellitus, dilated cardiomyopathy, and progressive hepatic and renal dysfunction.The cone-rod retinal dystrophy usually develops within a few weeks after birth. We examined a young boy with Alstrom by means of microperimetry MP-1 and optical coherence tomography (OCT) Spectral Domain. Instead of the typical alterations observed in cone-rod dystrophies, the characteristics of the central foveal tissue suggest signs of retinal immaturity, with only a single layer of short thick cones and rods as well as immature short outer segments. High- speed/ high- resolution spectral domain OCT allowed for the first time a detailed analysis of retinal layers in a young patient with Alstrom Syndrome. PMID:21158358

  4. Optical coherence tomography for high-resolution imaging of mouse development in utero

    NASA Astrophysics Data System (ADS)

    Syed, Saba H.; Larin, Kirill V.; Dickinson, Mary E.; Larina, Irina V.

    2011-04-01

    Although the mouse is a superior model to study mammalian embryonic development, high-resolution live dynamic visualization of mouse embryos remain a technical challenge. We present optical coherence tomography as a novel methodology for live imaging of mouse embryos through the uterine wall thereby allowing for time lapse analysis of developmental processes and direct phenotypic analysis of developing embryos. We assessed the capability of the proposed methodology to visualize structures of the living embryo from embryonic stages 12.5 to 18.5 days postcoitus. Repetitive in utero embryonic imaging is demonstrated. Our work opens the door for a wide range of live, in utero embryonic studies to screen for mutations and understand the effects of pharmacological and toxicological agents leading to birth defects.

  5. A low-cost, high-resolution, video-rate imaging optical radar

    SciTech Connect

    Sackos, J.T.; Nellums, R.O.; Lebien, S.M.; Diegert, C.F.; Grantham, J.W.; Monson, T.

    1998-04-01

    Sandia National Laboratories has developed a unique type of portable low-cost range imaging optical radar (laser radar or LADAR). This innovative sensor is comprised of an active floodlight scene illuminator and an image intensified CCD camera receiver. It is a solid-state device (no moving parts) that offers significant size, performance, reliability, and simplicity advantages over other types of 3-D imaging sensors. This unique flash LADAR is based on low cost, commercially available hardware, and is well suited for many government and commercial uses. This paper presents an update of Sandia`s development of the Scannerless Range Imager technology and applications, and discusses the progress that has been made in evolving the sensor into a compact, low, cost, high-resolution, video rate Laser Dynamic Range Imager.

  6. Fabrication of high-resolution gratings for polymeric optical waveguide devices

    NASA Astrophysics Data System (ADS)

    Shibata, Shinya; Sugihara, Okihiro; Kaino, Toshikuni; Okamoto, Naomichi

    2004-06-01

    High resolution gratings for the application of optical waveguide devices are fabricated using a series of photopolymers. The relief gratings were formed by the two-beam interference ablation technique using a third-harmonic generation of a Nd:YAG laser (355nm) onto polyimide and electrooptic polymer films. In polyimide films, the gratings with a period of 400nm and a depth of about 280nm were fabricated by the single-pulse irradiation. We tried to fabricate the gratings using a photoresist accompanied with wet development using an Ar+ laser (488nm). By wet development process, higher aspect and clearer periodical structure at a depth of 320nm and a period of nearly 500nm was realized. High diffraction efficiency of 55.4% was measured from the relief grating. We also replicated the grating to UV curable epoxy resin as an embossing master for the fabrication of waveguide devices.

  7. Continuous-wave whispering-gallery optical parametric oscillator for high-resolution spectroscopy.

    PubMed

    Werner, Christoph S; Buse, Karsten; Breunig, Ingo

    2015-03-01

    We achieve a continuous operation of a whispering gallery optical parametric oscillator by stabilizing the resonator temperature T on the mK level and simultaneously locking the pump frequency to a cavity resonance using the Pound-Drever-Hall technique. The millimeter-sized device converts several mW of a pump wave at 1040 nm wavelength to signal and idler waves around 2000 nm wavelength with more than 50% efficiency. Over 1 h, power and frequency of the signal wave vary by <±1% and by <±25  MHz, respectively. The latter can be tuned over 480 MHz without a mode hop by changing T over 120 mK. In order to prove the suitability for high-resolution spectroscopy, we scan the signal frequency across the resonance of a Fabry-Perot interferometer resolving nicely its 10 MHz linewidth.

  8. High resolution Fourier domain Optical Coherence Tomography at 2 microns for painted objects

    NASA Astrophysics Data System (ADS)

    Liang, H.; Cheung, C. S.; Daniel, J. M. O.; Tokurakawa, M.; Clarkson, W. A.; Spring, M.

    2015-06-01

    Optical Coherence Tomography has been successfully applied to the non-invasive imaging of subsurface microstructure of a variety of materials from biological tissues to painted objects of art. One of the limitations of the technique is the low depth of penetration due to the strong scattering and absorption in the material. Previous studies found that for paint materials, the optimum window for large depth of penetration is around 2.2 microns. This is also true for many other materials with low water content. We have previously demonstrated OCT systems in this wavelength regime for imaging with improved depth of penetration. In this paper, we present an improved 2 micron high resolution Fourier domain OCT system using a broadband supercontinuum source. The system achieved a depth resolution of 9 microns in air (or 6 microns in paint or any polymer).

  9. Kinematic Alignment and Bonding of Silicon Mirrors for High-Resolution Astronomical X-Ray Optics

    NASA Technical Reports Server (NTRS)

    Chan, Kai-Wing; Mazzarella, James R.; Saha, Timo T.; Zhang, William W.; Mcclelland, Ryan S.; Biskack, Michael P.; Riveros, Raul E.; Allgood, Kim D.; Kearney, John D.; Sharpe, Marton V.; hide

    2017-01-01

    Optics for the next generation's high-resolution, high throughput x-ray telescope requires fabrication of well-formed lightweight mirror segments and their integration at arc-second precision. Recent advances in the fabrication of silicon mirrors developed at NASA/Goddard prompted us to develop a new method of mirror alignment and integration. In this method, stiff silicon mirrors are aligned quasi-kinematically and are bonded in an interlocking fashion to produce a "meta-shell" with large collective area. We address issues of aligning and bonding mirrors with this method and show a recent result of 4 seconds-of-arc for a single pair of mirrors tested at soft x-rays.

  10. High-resolution full-field optical coherence tomography with a Linnik microscope.

    PubMed

    Dubois, Arnaud; Vabre, Laurent; Boccara, Alber-Claude; Beaurepaire, Emmanuel

    2002-02-01

    We describe an original microscope for high-resolution optical coherence tomography applications. Our system is based on a Linnik interference microscope with high-numerical-aperture objectives. Lock-in detection of the interference signal is achieved in parallel on a CCD by use of a photoelastic birefringence modulator and full-field stroboscopic illumination with an infrared LED. Transverse cross-section (en-face, or XY) images can be obtained in real time with better than 1-microm axial (Z) resolution and 0.5-microm transverse (XY) resolution. A sensitivity of approximately 80 dB is reached at a 1-image/s acquisition rate, which allows tomography in scattering media such as biological tissues.

  11. A Microscopic Optically Tracking Navigation System That Uses High-resolution 3D Computer Graphics.

    PubMed

    Yoshino, Masanori; Saito, Toki; Kin, Taichi; Nakagawa, Daichi; Nakatomi, Hirofumi; Oyama, Hiroshi; Saito, Nobuhito

    2015-01-01

    Three-dimensional (3D) computer graphics (CG) are useful for preoperative planning of neurosurgical operations. However, application of 3D CG to intraoperative navigation is not widespread because existing commercial operative navigation systems do not show 3D CG in sufficient detail. We have developed a microscopic optically tracking navigation system that uses high-resolution 3D CG. This article presents the technical details of our microscopic optically tracking navigation system. Our navigation system consists of three components: the operative microscope, registration, and the image display system. An optical tracker was attached to the microscope to monitor the position and attitude of the microscope in real time; point-pair registration was used to register the operation room coordinate system, and the image coordinate system; and the image display system showed the 3D CG image in the field-of-view of the microscope. Ten neurosurgeons (seven males, two females; mean age 32.9 years) participated in an experiment to assess the accuracy of this system using a phantom model. Accuracy of our system was compared with the commercial system. The 3D CG provided by the navigation system coincided well with the operative scene under the microscope. Target registration error for our system was 2.9 ± 1.9 mm. Our navigation system provides a clear image of the operation position and the surrounding structures. Systems like this may reduce intraoperative complications.

  12. A Microscopic Optically Tracking Navigation System That Uses High-resolution 3D Computer Graphics

    PubMed Central

    YOSHINO, Masanori; SAITO, Toki; KIN, Taichi; NAKAGAWA, Daichi; NAKATOMI, Hirofumi; OYAMA, Hiroshi; SAITO, Nobuhito

    2015-01-01

    Three-dimensional (3D) computer graphics (CG) are useful for preoperative planning of neurosurgical operations. However, application of 3D CG to intraoperative navigation is not widespread because existing commercial operative navigation systems do not show 3D CG in sufficient detail. We have developed a microscopic optically tracking navigation system that uses high-resolution 3D CG. This article presents the technical details of our microscopic optically tracking navigation system. Our navigation system consists of three components: the operative microscope, registration, and the image display system. An optical tracker was attached to the microscope to monitor the position and attitude of the microscope in real time; point-pair registration was used to register the operation room coordinate system, and the image coordinate system; and the image display system showed the 3D CG image in the field-of-view of the microscope. Ten neurosurgeons (seven males, two females; mean age 32.9 years) participated in an experiment to assess the accuracy of this system using a phantom model. Accuracy of our system was compared with the commercial system. The 3D CG provided by the navigation system coincided well with the operative scene under the microscope. Target registration error for our system was 2.9 ± 1.9 mm. Our navigation system provides a clear image of the operation position and the surrounding structures. Systems like this may reduce intraoperative complications. PMID:26226982

  13. Towards high-resolution retinal prostheses with direct optical addressing and inductive telemetry

    NASA Astrophysics Data System (ADS)

    Ha, Sohmyung; Khraiche, Massoud L.; Akinin, Abraham; Jing, Yi; Damle, Samir; Kuang, Yanjin; Bauchner, Sue; Lo, Yu-Hwa; Freeman, William R.; Silva, Gabriel A.; Cauwenberghs, Gert

    2016-10-01

    Objective. Despite considerable advances in retinal prostheses over the last two decades, the resolution of restored vision has remained severely limited, well below the 20/200 acuity threshold of blindness. Towards drastic improvements in spatial resolution, we present a scalable architecture for retinal prostheses in which each stimulation electrode is directly activated by incident light and powered by a common voltage pulse transferred over a single wireless inductive link. Approach. The hybrid optical addressability and electronic powering scheme provides separate spatial and temporal control over stimulation, and further provides optoelectronic gain for substantially lower light intensity thresholds than other optically addressed retinal prostheses using passive microphotodiode arrays. The architecture permits the use of high-density electrode arrays with ultra-high photosensitive silicon nanowires, obviating the need for excessive wiring and high-throughput data telemetry. Instead, the single inductive link drives the entire array of electrodes through two wires and provides external control over waveform parameters for common voltage stimulation. Main results. A complete system comprising inductive telemetry link, stimulation pulse demodulator, charge-balancing series capacitor, and nanowire-based electrode device is integrated and validated ex vivo on rat retina tissue. Significance. Measurements demonstrate control over retinal neural activity both by light and electrical bias, validating the feasibility of the proposed architecture and its system components as an important first step towards a high-resolution optically addressed retinal prosthesis.

  14. Multi-Sensor Fusion of Infrared and Electro-Optic Signals for High Resolution Night Images

    PubMed Central

    Huang, Xiaopeng; Netravali, Ravi; Man, Hong; Lawrence, Victor

    2012-01-01

    Electro-optic (EO) image sensors exhibit the properties of high resolution and low noise level at daytime, but they do not work in dark environments. Infrared (IR) image sensors exhibit poor resolution and cannot separate objects with similar temperature. Therefore, we propose a novel framework of IR image enhancement based on the information (e.g., edge) from EO images, which improves the resolution of IR images and helps us distinguish objects at night. Our framework superimposing/blending the edges of the EO image onto the corresponding transformed IR image improves their resolution. In this framework, we adopt the theoretical point spread function (PSF) proposed by Hardie et al. for the IR image, which has the modulation transfer function (MTF) of a uniform detector array and the incoherent optical transfer function (OTF) of diffraction-limited optics. In addition, we design an inverse filter for the proposed PSF and use it for the IR image transformation. The framework requires four main steps: (1) inverse filter-based IR image transformation; (2) EO image edge detection; (3) registration; and (4) blending/superimposing of the obtained image pair. Simulation results show both blended and superimposed IR images, and demonstrate that blended IR images have better quality over the superimposed images. Additionally, based on the same steps, simulation result shows a blended IR image of better quality when only the original IR image is available. PMID:23112602

  15. Near-Field Scanning Optical Microscopy for High-Resolution Membrane Studies

    PubMed Central

    Huckabay, Heath A.; Armendariz, Kevin P.; Newhart, William H.; Wildgen, Sarah M.; Dunn, Robert C.

    2012-01-01

    The desire to directly probe biological structures on the length scales that they exist has driven the steady development of various high-resolution microscopy techniques. Among these, optical microscopy and, in particular, fluorescence-based approaches continue to occupy dominant roles in biological studies given their favorable attributes. Fluorescence microscopy is both sensitive and specific, is generally noninvasive toward biological samples, has excellent temporal resolution for dynamic studies, and is relatively inexpensive. Light-based microscopies can also exploit a myriad of contrast mechanisms based on spectroscopic signatures, energy transfer, polarization, and lifetimes to further enhance the specificity or information content of a measurement. Historically, however, spatial resolution has been limited to approximately half the wavelength due to the diffraction of light. Near-field scanning optical microscopy (NSOM) is one of several optical approaches currently being developed that combines the favorable attributes of fluorescence microscopy with superior spatial resolution. NSOM is particularly well suited for studies of both model and biological membranes and application to these systems is discussed. PMID:23086886

  16. High-resolution optical imaging of functional brain architecture in the awake monkey.

    PubMed

    Grinvald, A; Frostig, R D; Siegel, R M; Bartfeld, E

    1991-12-15

    Optical imaging of the functional architecture of cortex, based on intrinsic signals, is a useful tool for the study of the development, organization, and function of the living mammalian brain. This relatively noninvasive technique is based on small activity-dependent changes of the optical properties of cortex. Thus far, functional imaging has been performed only on anesthetized animals. Here we establish that this technique is also suitable for exploring the brain of awake behaving primates. We designed a chronic sealed chamber and mounted it on the skull of a cynomolgus monkey (Macaca fascicularis) over the primary visual cortex to permit imaging through a transparent glass window. Restriction of head position alone was sufficient to eliminate movement noise in awake monkey imaging experiments. High-resolution imaging of the ocular dominance columns and the cytochrome oxidase blobs was achieved simply by taking pictures of the exposed cortex when the awake monkey was viewing video movies alternatively with each eye. Furthermore, the functional maps could be obtained without synchronization of the data acquisition to the animal's respiration and the electrocardiogram. The wavelength dependency and time course of the intrinsic signal were similar in anesthetized and awake monkeys, indicating that the signal sources were the same. We therefore conclude that optical imaging is well suited for exploring functional organization related to higher cognitive brain functions of the primate as well as providing a diagnostic tool for delineating functional cortical borders and assessing proper functions of human patients during neurosurgery.

  17. [Study on an optical system of small ultraviolet imaging spectrometer with high resolution in broadband].

    PubMed

    Cong, Hai-Fang; Wang, Chun-Hui; Wang, Yu

    2013-02-01

    An ultraviolet imaging spectrometer was studied based on the principle of the small scale ultraviolet spectral instrument. The scheme composed of an off-axis parabolic mirror telescope and a single toroidal grating spectral imaging system was designed. The optimization of the optical system is the optimum processing for the parameters of the toroidal grating. The optical path function and the aberration equations of the grating were analyzed. The perfect anastigmatism conditions and imaging conditions of the single toroidal grating system were obtained. These two conditions that cannot be satisfied by the algebra calculation method limit the field of view and waveband of the spectrometer. The genetic algorithm was introduced to solve the problem. A solar-blind ultraviolet imaging spectrometer for 200-280 nm was designed to verify the design method. The optimum initial configuration was calculated and simulated. A system with F/# 5.7, focal length 102 mm and high spatial resolution was designed. The modulation transfer functions (MTF) of all fields of view are more than 0.65 in the waveband in the required Nyquist frequency (20 1p x mm(-1)). The design results indicate that the optical system theory can be applied to the small scale ultraviolet imaging spectrometer with high resolution and spectral broadband.

  18. Multi-sensor fusion of infrared and electro-optic signals for high resolution night images.

    PubMed

    Huang, Xiaopeng; Netravali, Ravi; Man, Hong; Lawrence, Victor

    2012-01-01

    Electro-optic (EO) image sensors exhibit the properties of high resolution and low noise level at daytime, but they do not work in dark environments. Infrared (IR) image sensors exhibit poor resolution and cannot separate objects with similar temperature. Therefore, we propose a novel framework of IR image enhancement based on the information (e.g., edge) from EO images, which improves the resolution of IR images and helps us distinguish objects at night. Our framework superimposing/blending the edges of the EO image onto the corresponding transformed IR image improves their resolution. In this framework, we adopt the theoretical point spread function (PSF) proposed by Hardie et al. for the IR image, which has the modulation transfer function (MTF) of a uniform detector array and the incoherent optical transfer function (OTF) of diffraction-limited optics. In addition, we design an inverse filter for the proposed PSF and use it for the IR image transformation. The framework requires four main steps: (1) inverse filter-based IR image transformation; (2) EO image edge detection; (3) registration; and (4) blending/superimposing of the obtained image pair. Simulation results show both blended and superimposed IR images, and demonstrate that blended IR images have better quality over the superimposed images. Additionally, based on the same steps, simulation result shows a blended IR image of better quality when only the original IR image is available.

  19. High-resolution laser lithography system based on two-dimensional acousto-optic deflection

    NASA Astrophysics Data System (ADS)

    Koechlin, Manuel; Poberaj, Gorazd; Günter, Peter

    2009-08-01

    We present an advanced high-resolution, compact laser lithography system for fast prototyping of complex integrated optics devices comprising microring resonators and photonic crystal structures. Precise and flexible structuring of photoresist patterns is achieved by combing three linear stages (xyz) for sample positioning and a two-dimensional acousto-optical deflector for laser beam steering and intensity control. A continuous wave diode laser operating at a wavelength of 375 nm is used to illuminate all types of photoresists including SU-8. Using a microscope objective with a numerical aperture of 1.40, structure widths of ˜200 nm can be obtained. The write-field covered by acousto-optic deflection can be as large as 200×200 μm2 when using an objective with a focal length of 4.5 mm. With a two-step lithography process, gaps as small as 150 nm between adjacent structures have been achieved, yielding superior photoresist masks for microring resonators with coupling ports.

  20. First steps toward 3D high resolution imaging using adaptive optics and full-field optical coherence tomography

    NASA Astrophysics Data System (ADS)

    Blanco, Leonardo; Blavier, Marie; Glanc, Marie; Pouplard, Florence; Tick, Sarah; Maksimovic, Ivan; Chenegros, Guillaume; Mugnier, Laurent; Lacombe, Francois; Rousset, Gérard; Paques, Michel; Le Gargasson, Jean-François; Sahel, Jose-Alain

    2008-09-01

    We describe here two parts of our future 3D fundus camera coupling Adaptive Optics and full-field Optical Coherence Tomography. The first part is an Adaptive Optics flood imager installed at the Quinze-Vingts Hospital, regularly used on healthy and pathological eyes. A posteriori image reconstruction is performed, increasing the final image quality and field of view. The instrument lateral resolution is better than 2 microns. The second part is a full-field Optical Coherence Tomograph, which has demonstrated capability of performing a simple kind of "4 phases" image reconstruction of non biological samples and ex situ retinas. Final aim is to couple both parts in order to achieve 3D high resolution mapping of in vivo retinas.

  1. Meta-shell Approach for Constructing Lightweight and High Resolution X-Ray Optics

    NASA Technical Reports Server (NTRS)

    McClelland, Ryan S.

    2016-01-01

    Lightweight and high resolution optics are needed for future space-based x-ray telescopes to achieve advances in high-energy astrophysics. Past missions such as Chandra and XMM-Newton have achieved excellent angular resolution using a full shell mirror approach. Other missions such as Suzaku and NuSTAR have achieved lightweight mirrors using a segmented approach. This paper describes a new approach, called meta-shells, which combines the fabrication advantages of segmented optics with the alignment advantages of full shell optics. Meta-shells are built by layering overlapping mirror segments onto a central structural shell. The resulting optic has the stiffness and rotational symmetry of a full shell, but with an order of magnitude greater collecting area. Several meta-shells so constructed can be integrated into a large x-ray mirror assembly by proven methods used for Chandra and XMM-Newton. The mirror segments are mounted to the meta-shell using a novel four point semi-kinematic mount. The four point mount deterministically locates the segment in its most performance sensitive degrees of freedom. Extensive analysis has been performed to demonstrate the feasibility of the four point mount and meta-shell approach. A mathematical model of a meta-shell constructed with mirror segments bonded at four points and subject to launch loads has been developed to determine the optimal design parameters, namely bond size, mirror segment span, and number of layers per meta-shell. The parameters of an example 1.3 m diameter mirror assembly are given including the predicted effective area. To verify the mathematical model and support opto-mechanical analysis, a detailed finite element model of a meta-shell was created. Finite element analysis predicts low gravity distortion and low thermal distortion. Recent results are discussed including Structural Thermal Optical Performance (STOP) analysis as well as vibration and shock testing of prototype meta-shells.

  2. High-resolution imaging spectrometer for recording absolutely calibrated far ultraviolet spectra from laser-produced plasmas

    SciTech Connect

    Brown, Charles M.; Seely, John F.; Feldman, Uri; Holland, Glenn E.; Weaver, James L.; Obenschain, Steven P.; Kjornrattanawanich, Benjawan; Fielding, Drew

    2008-10-15

    An imaging spectrometer was designed and fabricated for recording far ultraviolet spectra from laser-produced plasmas with wavelengths as short as 155 nm. The spectrometer implements a Cassegrain telescope and two gratings in a tandem Wadsworth optical configuration that provides diffraction limited resolution. Spectral images were recorded from plasmas produced by the irradiation of various target materials by intense KrF laser radiation with 248 nm wavelength. Two pairs of high-resolution gratings can be selected for the coverage of two wavebands, one grating pair with 1800 grooves/mm and covering approximately 155-175 nm and another grating pair with 1200 grooves/mm covering 230-260 nm. The latter waveband includes the 248 nm KrF laser wavelength, and the former waveband includes the wavelength of the two-plasmon decay instability at (2/3) the KrF laser wavelength (165 nm). The detection media consist of a complementary metal oxide semiconductor imager, photostimulable phosphor image plates, and a linear array of 1 mm{sup 2} square silicon photodiodes with 0.4 ns rise time. The telescope mirrors, spectrometer gratings, and 1 mm{sup 2} photodiode were calibrated using synchrotron radiation, and this enables the measurement of the absolute emission from the laser-produced plasmas with temporal, spatial, and spectral resolutions. The spectrometer is capable of measuring absolute spectral emissions at 165 nm wavelength as small as 5x10{sup -7} J/nm from a plasma source area of 0.37 mm{sup 2} and with 0.4 ns time resolution.

  3. Fiber Optic Distributed Sensors for High-resolution Temperature Field Mapping

    PubMed Central

    Lomperski, Stephen; Gerardi, Craig; Lisowski, Darius

    2016-01-01

    The reliability of computational fluid dynamics (CFD) codes is checked by comparing simulations with experimental data. A typical data set consists chiefly of velocity and temperature readings, both ideally having high spatial and temporal resolution to facilitate rigorous code validation. While high resolution velocity data is readily obtained through optical measurement techniques such as particle image velocimetry, it has proven difficult to obtain temperature data with similar resolution. Traditional sensors such as thermocouples cannot fill this role, but the recent development of distributed sensing based on Rayleigh scattering and swept-wave interferometry offers resolution suitable for CFD code validation work. Thousands of temperature measurements can be generated along a single thin optical fiber at hundreds of Hertz. Sensors function over large temperature ranges and within opaque fluids where optical techniques are unsuitable. But this type of sensor is sensitive to strain and humidity as well as temperature and so accuracy is affected by handling, vibration, and shifts in relative humidity. Such behavior is quite unlike traditional sensors and so unconventional installation and operating procedures are necessary to ensure accurate measurements. This paper demonstrates implementation of a Rayleigh scattering-type distributed temperature sensor in a thermal mixing experiment involving two air jets at 25 and 45 °C. We present criteria to guide selection of optical fiber for the sensor and describe installation setup for a jet mixing experiment. We illustrate sensor baselining, which links readings to an absolute temperature standard, and discuss practical issues such as errors due to flow-induced vibration. This material can aid those interested in temperature measurements having high data density and bandwidth for fluid dynamics experiments and similar applications. We highlight pitfalls specific to these sensors for consideration in experiment design

  4. A new method of inshore ship detection in high-resolution optical remote sensing images

    NASA Astrophysics Data System (ADS)

    Hu, Qifeng; Du, Yaling; Jiang, Yunqiu; Ming, Delie

    2015-10-01

    Ship as an important military target and water transportation, of which the detection has great significance. In the military field, the automatic detection of ships can be used to monitor ship dynamic in the harbor and maritime of enemy, and then analyze the enemy naval power. In civilian field, the automatic detection of ships can be used in monitoring transportation of harbor and illegal behaviors such as illegal fishing, smuggling and pirates, etc. In recent years, research of ship detection is mainly concentrated in three categories: forward-looking infrared images, downward-looking SAR image, and optical remote sensing images with sea background. Little research has been done into ship detection of optical remote sensing images with harbor background, as the gray-scale and texture features of ships are similar to the coast in high-resolution optical remote sensing images. In this paper, we put forward an effective harbor ship target detection method. First of all, in order to overcome the shortage of the traditional difference method in obtaining histogram valley as the segmentation threshold, we propose an iterative histogram valley segmentation method which separates the harbor and ships from the water quite well. Secondly, as landing ships in optical remote sensing images usually lead to discontinuous harbor edges, we use Hough Transform method to extract harbor edges. First, lines are detected by Hough Transform. Then, lines that have similar slope are connected into a new line, thus we access continuous harbor edges. Secondary segmentation on the result of the land-and-sea separation, we eventually get the ships. At last, we calculate the aspect ratio of the ROIs, thereby remove those targets which are not ship. The experiment results show that our method has good robustness and can tolerate a certain degree of noise and occlusion.

  5. Fiber Optic Distributed Sensors for High-resolution Temperature Field Mapping.

    PubMed

    Lomperski, Stephen; Gerardi, Craig; Lisowski, Darius

    2016-11-07

    The reliability of computational fluid dynamics (CFD) codes is checked by comparing simulations with experimental data. A typical data set consists chiefly of velocity and temperature readings, both ideally having high spatial and temporal resolution to facilitate rigorous code validation. While high resolution velocity data is readily obtained through optical measurement techniques such as particle image velocimetry, it has proven difficult to obtain temperature data with similar resolution. Traditional sensors such as thermocouples cannot fill this role, but the recent development of distributed sensing based on Rayleigh scattering and swept-wave interferometry offers resolution suitable for CFD code validation work. Thousands of temperature measurements can be generated along a single thin optical fiber at hundreds of Hertz. Sensors function over large temperature ranges and within opaque fluids where optical techniques are unsuitable. But this type of sensor is sensitive to strain and humidity as well as temperature and so accuracy is affected by handling, vibration, and shifts in relative humidity. Such behavior is quite unlike traditional sensors and so unconventional installation and operating procedures are necessary to ensure accurate measurements. This paper demonstrates implementation of a Rayleigh scattering-type distributed temperature sensor in a thermal mixing experiment involving two air jets at 25 and 45 °C. We present criteria to guide selection of optical fiber for the sensor and describe installation setup for a jet mixing experiment. We illustrate sensor baselining, which links readings to an absolute temperature standard, and discuss practical issues such as errors due to flow-induced vibration. This material can aid those interested in temperature measurements having high data density and bandwidth for fluid dynamics experiments and similar applications. We highlight pitfalls specific to these sensors for consideration in experiment design

  6. Research on the flywheel components' disturbance mechanism of a high resolution optical satellite

    NASA Astrophysics Data System (ADS)

    Lin, Li; Dong, Wang; Sitong, Zhou; Tan, Luyang

    2016-10-01

    According to the picture of a sub-meter resolution optical satellite acquired on the orbit, there is a phenomenon of jitter in the process of taking pictures. The flywheel as the main attitude control component of the satellite, the disturbance that it caused has great influence on the high resolution optical satellite in its normal action. This paper has respectively researched the flywheel components' disturbance mechanism from three parts, including uneven rotator, rotator friction, bearing disturbance, builds the mathematics model of disturbance to analysis the characteristic of disturbance. we get that the vibration system is not a fully linear system, the system is linear before the occurrence of rubbing. It also can be seen that the system has a number of different cross rigidity, it will often appear unstable motion that resulting in damage, or becomes the ultimate destruction due to the role of nonlinear damping. When the rolling roll in the surface, it will produce an alternative excitation force if there exist defects or damage in the rolling surface. This research would offer guidance for system optimization design and vibrating isolation compensation of the later type of improved satellite.

  7. High-resolution imaging characterization of bladder dynamic morphophysiology by time-lapse optical coherence tomography

    NASA Astrophysics Data System (ADS)

    Pan, Y. T.; Wu, Q.; Wang, Z. G.; Brink, P. R.; Du, C. W.

    2005-09-01

    We report an experimental study of the possibility of high-speed optical coherence tomography (OCT) for high-resolution imaging characterization of detrusor dynamic morphophysiology and analysis of the mechanisms that lead to geriatric incontinence (GI). The spontaneous contractility of intact fresh rabbit bladders was imaged with two-dimensional (2D) OCT ex vivo at up to 8frames/s. The time-lapse 2D OCT images were postprocessed by image segmentation and fast-Fourier-transform analysis to characterize the dynamic morphological changes of the bladder contractility. In addition, we studied young and aging rat bladders to analyze the differences in dynamics. Preliminary results of our ex vivo study reveal that time-lapse OCT can track the contractile waves of bladders at high spatial resolution and characterize their dynamic morphophysiology in terms of amplitude, phase, and frequency. The results suggest that time-lapse OCT has the potential to act as a detrusor optical biopsy to enhance the diagnosis of detrusor dysfunction and thus of the mechanisms that lead to GI.

  8. Diagnosis of Ocular Surface Lesions Using Ultra-High Resolution Optical Coherence Tomography

    PubMed Central

    Shousha, Mohamed Abou; Karp, Carol L.; Canto, Ana Paula; Hodson, Kelly; Oellers, Patrick; Kao, Andrew A.; Bielory, Brett; Matthews, Jared; Dubovy, Sander R.; Perez, Victor L.; Wang, Jianhua

    2012-01-01

    Purpose To assess the use of ultra high resolution optical coherence tomography (UHR-OCT) in the diagnosis of ocular surface lesions. Design Prospective, non-comparative, interventional case series. Participants Fifty four eyes of 53 consecutive patients with biopsy proven ocular surface lesions; 8 primary acquired melanosis, 5 amelanotic melanoma, 2 nevi, 19 ocular surface squamous neoplasia, 1 histiocytosis, 6 conjunctival lymphoma, 2 conjunctival amyloidosis, and 11 pterygia. Intervention UHR-OCT imaging of the ocular surface lesions. Main Outcome Measures Clinical course and photographs, UHR-OCT image and histopathological findings. Results UHR-OCT images of all examined ocular surface lesions showed close correlation with the obtained histopathological specimens. When clinical differential diagnosis of ocular surface lesions was broad, UHR-OCT images provided optical signs that guided towards a more specific diagnosis and management. In cases of amelanotic melanoma, conjunctival amyloidosis, and primary histiocytosis and in one case of ocular surface squamous neoplasia, UHR-OCT was instrumental in guiding the diagnosis. In those cases, UHR-OCT suggested that the presumed clinical diagnosis was incorrect and favored a diagnosis which was later confirmed by histopathological examination. Conclusions Correlations between UHR-OCT and histopathology confirm that UHR-OCT is an adjunctive diagnostic modality that can provide a non-invasive means to help and guide diagnosis and management of ocular surface lesions. PMID:23347984

  9. Optical design of the PEPSI high-resolution spectrograph at LBT

    NASA Astrophysics Data System (ADS)

    Andersen, Michael I.; Spano, Paolo; Woche, Manfred; Strassmeier, Klaus G.; Beckert, Erik

    2004-09-01

    PEPSI is a high-resolution, fiber fed echelle spectrograph with polarimetric capabilities for the LBT. In order to reach a maximum resolution R=120.000 in polarimetric mode and 300.000 in integral light mode with high efficiency in the spectral range 390-1050~nm, we designed a white-pupil configuration with Maksutov collimators. Light is dispersed by an R4 31.6 lines/mm monolithic echelle grating mosaic and split into two arms through dichroics. The two arms, optimized for the spectral range 390-550~nm and 550-1050~nm, respectively, consist of Maksutov transfer collimators, VPH-grism cross dispersers, optimized dioptric cameras and 7.5K x 7.5K 8~μ CCDs. Fibers of different core sizes coupled to different image-slicers allow a high throughput, comparable to that of direct feed instruments. The optical configuration with only spherical and cylindrical surfaces, except for one aspherical surface in each camera, reduces costs and guarantees high optical quality. PEPSI is under construction at AIP with first light expected in 2006.

  10. Pupil-transformation multiconjugate adaptive optics for solar high-resolution imaging

    NASA Astrophysics Data System (ADS)

    Ren, Deqing; Zhang, Xi; Dou, Jiangpei; Zhu, Yongtian; Broadfoot, Robert; Chapman, Julius

    2016-09-01

    We propose a multiconjugate adaptive optics (MCAO) system called pupil-transformation MCAO (PT-MCAO) for solar high-angular resolution imaging over a large field of view. The PT-MCAO, consisting of two deformable mirrors (DMs), uses a Shack-Hartmann wavefront sensor located on the telescope pupil to measure the wavefront slopes from several guide stars. The average slopes are used to control the first DM conjugated on the telescope aperture by a solar ground-layer adaptive optics (AO) approach while the remaining slopes are used to control the second DM conjugated on a high altitude by a conventional solar AO via a geometric PT. The PT-MCAO uses a similar hardware configuration as the conventional star-oriented MCAO. However, a distinctive feature of our PT-MCAO is that it avoids the construction of tomography wavefront, which is a time-consuming and complex process for the solar real-time atmospheric turbulence correction. For the PT-MCAO, current widely used and fully understood conventional solar AO closed-loop control algorithms can be directly used to control the two DMs, which greatly reduces the real-time calculation power requirement and makes the PT-MCAO easy to implement. In this publication, we discuss the PT-MCAO methodology, its unique features, and compare its performance with that of the conventional solar star-oriented MCAO systems, which demonstrate that the PT-MCAO can be immediately used for solar high-resolution imaging.

  11. High-resolution retinal imaging using adaptive optics and Fourier-domain optical coherence tomography

    DOEpatents

    Olivier, Scot S.; Werner, John S.; Zawadzki, Robert J.; Laut, Sophie P.; Jones, Steven M.

    2010-09-07

    This invention permits retinal images to be acquired at high speed and with unprecedented resolution in three dimensions (4.times.4.times.6 .mu.m). The instrument achieves high lateral resolution by using adaptive optics to correct optical aberrations of the human eye in real time. High axial resolution and high speed are made possible by the use of Fourier-domain optical coherence tomography. Using this system, we have demonstrated the ability to image microscopic blood vessels and the cone photoreceptor mosaic.

  12. High-resolution differential mode delay measurement for a multimode optical fiber using a modified optical frequency domain reflectometer.

    PubMed

    Ahn, T-J; Kim, D

    2005-10-03

    A novel differential mode delay (DMD) measurement technique for a multimode optical fiber based on optical frequency domain reflectometry (OFDR) has been proposed. We have obtained a high-resolution DMD value of 0.054 ps/m for a commercial multimode optical fiber with length of 50 m by using a modified OFDR in a Mach-Zehnder interferometer structure with a tunable external cavity laser and a Mach-Zehnder interferometer instead of Michelson interferometer. We have also compared the OFDR measurement results with those obtained using a traditional time-domain measurement method. DMD resolution with our proposed OFDR technique is more than an order of magnitude better than a result obtainable with a conventional time-domain method.

  13. Multidisciplinary study Of Continental/ocean Climate dynamics using High-resolution records from the eastern mediterraneAn (MOCCHA)

    NASA Astrophysics Data System (ADS)

    de Lange, G. J.; Versteegh, G.; Zonneveld, K. A. F.; Bernasconi, S. M.

    2009-04-01

    For high-resolution paleoclimate studies on high-frequency variations, continuous marine records with sufficient time resolution are needed. Such records are rare but vital for our understanding of causes and consequences of climate and environmental change at decadal to millennial time scales. Our initial studies at a near-coastal and a deep Mediterranean anoxic basin site seem to provide a continuous marine paleo-climate record that permits such high-resolution and well dated climate reconstructions for at least the last few kyrs. Cores for the MOCCHA project have been collected during the pre-Moccha ESPRESSO cruise with RV Universitatis and CAPUCCINO cruise with RV Poseidon, followed by the DOPPIO cruise with RV Pelagia. The cores recovered and studied thusfar appear to contain largely laminated sediments (submillimetric) down to 10 kyr. We will introduce the sites with existing and recently published evidence and supplement these with preliminary results for both sites obtained during these cruises. All of these are illustrating their suitability for high-resolution studies of paleoclimate that we hope to extend to > 35 kyr, i.e. for future IODP drilling. This work is supported by the EUROMARGINS Programme of the European Science Foundation NWO.817.01.002 MOCCHA project).

  14. Multilayer Optics for Ultra-high Resolution Solar Imaging in the EUV Region

    NASA Astrophysics Data System (ADS)

    Soufli, R.; Spiller, E.; Sommargren, G. E.; Bajt, S.; Folta, J. A.; Taylor, J. S.; Gullikson, E. M.

    2003-05-01

    Highly reflective multilayer-coated optics operating at near-normal incidence angles have been the enabling technology for solar imaging instruments in the extreme ultraviolet (EUV) energy range. Despite the advances made in recent years towards understanding of solar processes through missions such as TRACE, major unresolved questions in solar physics still remain, for instance on the subjects of coronal heating, eruptive flare and coronal wind initiation. Future generations of missions will need to study the physics of hot magnetized plasmas that occur in the corona on extremely small spatial and temporal scales, requiring imaging instruments with extremely high resolution and large fields of view. Proposals for future solar missions require optics with diameters up to 700 mm and system wavefront errors as low as 0.4 nm. Experimental results will be presented for normal-incidence, four-mirror and two-mirror EUV cameras operating around 13.4 nm. Mirror substrates were manufactured by commercial vendors and achieved figure errors around 0.25 nm rms, verified by phase-shifting, point-diffraction visible light interferometers developed at Lawrence Livermore Lab. The optics were multilayer-coated aligned and tested at facilities at Lawrence Livermore and Lawrence Berkeley National Labs. A large-scale DC-magnetron sputtering tool is used to coat the optics and can accommodate multiple optics up to 600 mm in diameter in a single deposition run. During multilayer deposition, a velocity modulation algorithm is applied in order to achieve extremely precise film thickness control. The deposited Mo/Si coatings demonstrate added figure errors below 0.05 nm rms. While these systems were constructed for EUV lithographic applications, the experimental results are immediately applicable to astronomical x-ray optics. Currently these are the only multilayer-coated EUV cameras worldwide meeting such stringent specifications, and have been implemented in the construction of the first

  15. High-resolution flying-PIV with optical fiber laser delivery

    NASA Astrophysics Data System (ADS)

    Weichselbaum, Noah A.; André, Matthieu A.; Rahimi-Abkenar, Morteza; Manzari, Majid T.; Bardet, Philippe M.

    2016-05-01

    Implementation of non-intrusive optical measurement techniques, such as particle image velocimetry (PIV), in harsh environments requires specialized techniques for introducing controlled laser sheets to the region of interest. Large earthquake shake tables are a particularly challenging environment. Lasers must be mounted away from the table, and the laser sheet has to be delivered precisely and stably to the measurement station. Here, high-power multi-mode step-index fiber optics enable introduction of light from an Nd:YLF pulsed laser to a remote test section. Such lasers are suitable for coupling to optical fibers, which presents a portable, flexible, and safe manner to deliver a PIV light sheet. Best practices for their implementation are reviewed. Particular attention is focused on obtaining a collimated beam of acceptable quality at the output of the fiber. To achieve high spatial resolution, the PIV camera is directly mounted on the moving shake table with care to minimize its vibrations. A special arrangement of PIV planes is deployed for precise in-situ PIV alignment and to monitor and account for residual structure vibrations and beam wandering. The design of the instruments is detailed. Here, an experimental facility for the study of nuclear fuel bundle response to seismic forcing near prototypical conditions is instrumented. Only through integration of a high-resolution flying-PIV system can velocity fields be acquired. Data indicate that in the presence of a mean axial flow, a secondary oscillatory flow develops as the bundle oscillates. Instantaneous, phase-averaged, and fluctuating velocity fields illustrate this phenomenon.

  16. High-resolution full-field optical coherence tomography using high dynamic range image processing

    NASA Astrophysics Data System (ADS)

    Leong-Hoï, A.; Claveau, R.; Montgomery, P. C.; Serio, B.; Uhring, W.; Anstotz, F.; Flury, M.

    2016-04-01

    Full-field optical coherence tomography (FF-OCT) based on white-light interference microscopy, is an emerging noninvasive imaging technique for characterizing biological tissue or optical scattering media with micrometer resolution. Tomographic images can be obtained by analyzing a sequence of interferograms acquired with a camera. This is achieved by scanning an interferometric microscope objectives along the optical axis and performing appropriate signal processing for fringe envelope extraction, leading to three-dimensional imaging over depth. However, noise contained in the images can hide some important details or induce errors in the size of these details. To firstly reduce temporal and spatial noise from the camera, it is possible to apply basic image post processing methods such as image averaging, dark frame subtraction or flat field division. It has been demonstrate that this can improve the quality of microscopy images by enhancing the signal to noise ratio. In addition, the dynamic range of images can be enhanced to improve the contrast by combining images acquired with different exposure times or light intensity. This can be made possible by applying a hybrid high dynamic range (HDR) technique, which is proposed in this paper. High resolution tomographic analysis is thus performed using a combination of the above-mentioned image processing techniques. As a result, the lateral resolution of the system can be improved so as to approach the diffraction limit of the microscope as well as to increase the power of detection, thus enabling new sub-diffraction sized structures contained in a transparent layer, initially hidden by the noise, to be detected.

  17. Suitability of satellite derived and gridded sea surface temperature data sets for calibrating high-resolution marine proxy records

    NASA Astrophysics Data System (ADS)

    Ouellette, G., Jr.; DeLong, K. L.

    2016-02-01

    High-resolution proxy records of sea surface temperature (SST) are increasingly being produced using trace element and isotope variability within the skeletal materials of marine organisms such as corals, mollusks, sclerosponges, and coralline algae. Translating the geochemical variations within these organisms into records of SST requires calibration with SST observations using linear regression methods, preferably with in situ SST records that span several years. However, locations with such records are sparse; therefore, calibration is often accomplished using gridded SST data products such as the Hadley Center's HADSST (5º) and interpolated HADISST (1º) data sets, NOAA's extended reconstructed SST data set (ERSST; 2º), optimum interpolation SST (OISST; 1º), and Kaplan SST data sets (5º). From these data products, the SST used for proxy calibration is obtained for a single grid cell that includes the proxy's study site. The gridded data sets are based on the International Comprehensive Ocean-Atmosphere Data Set (ICOADS) and each uses different methods of interpolation to produce the globally and temporally complete data products except for HadSST, which is not interpolated but quality controlled. This study compares SST for a single site from these gridded data products with a high-resolution satellite-based SST data set from NOAA (Pathfinder; 4 km) with in situ SST data and coral Sr/Ca variability for our study site in Haiti to assess differences between these SST records with a focus on seasonal variability. Our results indicate substantial differences in the seasonal variability captured for the same site among these data sets on the order of 1-3°C. This analysis suggests that of the data products, high-resolution satellite SST best captured seasonal variability at the study site. Unfortunately, satellite SST records are limited to the past few decades. If satellite SST are to be used to calibrate proxy records, collecting modern, living samples is

  18. Insulated Conducting Cantilevered Nanotips and Two-Chamber Recording System for High Resolution Ion Sensing AFM

    PubMed Central

    Meckes, Brian; Arce, Fernando Teran; Connelly, Laura S.; Lal, Ratnesh

    2014-01-01

    Biological membranes contain ion channels, which are nanoscale pores allowing controlled ionic transport and mediating key biological functions underlying normal/abnormal living. Synthetic membranes with defined pores are being developed to control various processes, including filtration of pollutants, charge transport for energy storage, and separation of fluids and molecules. Although ionic transport (currents) can be measured with single channel resolution, imaging their structure and ionic currents simultaneously is difficult. Atomic force microscopy enables high resolution imaging of nanoscale structures and can be modified to measure ionic currents simultaneously. Moreover, the ionic currents can also be used to image structures. A simple method for fabricating conducting AFM cantilevers to image pore structures at high resolution is reported. Tungsten microwires with nanoscale tips are insulated except at the apex. This allows simultaneous imaging via cantilever deflections in normal AFM force feedback mode as well as measuring localized ionic currents. These novel probes measure ionic currents as small as picoampere while providing nanoscale spatial resolution surface topography and is suitable for measuring ionic currents and conductance of biological ion channels. PMID:24663394

  19. New composite blue sensitive materials for high resolution optical data storage

    NASA Astrophysics Data System (ADS)

    Criante, L.; Castagna, R.; Vita, F.; Lucchetta, D. E.; Simoni, F.; Frohmann, S.; Feid, T.; Orlic, S.

    2007-09-01

    In the last three decades several kinds of organic mixtures for holographic recording were developed in order to achieve a new class of DVD-like optical memories for high-density optical data storage. The holographic materials should satisfy the following requirements: high sensitivity to blue light, low losses, high spatial resolution and long term stability. To this aim we developed new organic photosensitive mixtures based on only three components. We recorded high spatial frequency reflection gratings up to 7400 lines/mm with blue laser light (405 nm) by using a conventional holographic setup. We obtained a macro grating diffraction efficiency up to 67%, refractive index modulation over 0.01, optical shrinkage < 2 % and overall losses ~5%. In order to characterize data-storage materials independently on the experimental conditions, the sensitivity has been evaluated through the S parameter which takes into account the diffraction efficiency, recording light intensity, exposure time and sample thickness. The amazing obtained values of S >10 5 cm/J evidences a very fast recording process with a very low writing intensity (less than 20 mW/cm2) corresponding to a recording energy density of few mJ/cm2. The performance of these materials have been also tested in the microholographic geometry.

  20. High resolution retinal imaging with a compact adaptive optics spectral domain optical coherence tomography system

    NASA Astrophysics Data System (ADS)

    Hammer, Daniel X.; Iftimia, Nicusor V.; Bigelow, Chad E.; Ustun, Teoman E.; Bloom, Benjamin; Ferguson, R. Daniel; Burns, Stephen A.

    2007-02-01

    Adaptive optics (AO) is used to correct ocular aberrations primarily in the cornea, lens, and tear film of every eye. Among other applications, AO allows high lateral resolution images to be acquired with scanning laser ophthalmoscopy (SLO) and optical coherence tomography (OCT). Spectral domain optical coherence tomography (SDOCT) is a high-speed imaging technique that can acquire cross-sectional scans with micron-scale axial resolution at tens to hundreds of kHz line rates. We present a compact clinical AO-SDOCT system that achieves micron-scale axial and lateral resolution of retinal structures. The system includes a line scanning laser ophthalmscope (LSLO) for simultaneous wide-field retinal viewing and selection of regions-of-interest. OCT and LSLO imaging and AO correction performance are characterized. We present a case study of a single subject with hyper-reflective lesions associated with stable, resolved central serous retinopathy to compare and contrast AO as applied to scanning laser ophthalmoscopy and optical coherence tomography. The two imaging modes are found to be complementary in terms of information on structure morphology. Both provide additional information lacking in the other. This preliminary finding points to the power of combining SLO and SDOCT in a single research instrument for exploration of disease mechanisms, retinal cellular architecture, and visual psychophysics.

  1. High-resolution optical coherence tomographic imaging of osteoarthritic cartilage during open knee surgery

    PubMed Central

    Li, Xingde; Martin, Scott; Pitris, Costas; Ghanta, Ravi; Stamper, Debra L; Harman, Michelle; Fujimoto, James G; Brezinski, Mark E

    2005-01-01

    This study demonstrates the first real-time imaging in vivo of human cartilage in normal and osteoarthritic knee joints at a resolution of micrometers, using optical coherence tomography (OCT). This recently developed high-resolution imaging technology is analogous to B-mode ultrasound except that it uses infrared light rather than sound. Real-time imaging with 11-μm resolution at four frames per second was performed on six patients using a portable OCT system with a handheld imaging probe during open knee surgery. Tissue registration was achieved by marking sites before imaging, and then histologic processing was performed. Structural changes including cartilage thinning, fissures, and fibrillations were observed at a resolution substantially higher than is achieved with any current clinical imaging technology. The structural features detected with OCT were evident in the corresponding histology. In addition to changes in architectural morphology, changes in the birefringent or the polarization properties of the articular cartilage were observed with OCT, suggesting collagen disorganization, an early indicator of osteoarthritis. Furthermore, this study supports the hypothesis that polarization-sensitive OCT may allow osteoarthritis to be diagnosed before cartilage thinning. This study illustrates that OCT, which can eventually be developed for use in offices or through an arthroscope, has considerable potential for assessing early osteoarthritic cartilage and monitoring therapeutic effects for cartilage repair with resolution in real time on a scale of micrometers. PMID:15743479

  2. Multilayer optics for monochromatic high-resolution x-ray imaging mircoscopes

    NASA Astrophysics Data System (ADS)

    Troussel, Ph.; Do, A.; Gontier, D.; Dennetiere, D.; Høghøj, P.; Hedacq, S.

    2015-08-01

    Within the framework of its researches on Inertial Confinement Fusion (ICF), the "Commissariat à l'Énergie Atomique et aux Énergies Alternatives" (CEA) studies and designs advanced X-ray diagnostics in order to probe dense plasmas produced by Laser facilities. The final goal for those diagnostics is to be used during experiments on the Laser Megajoules french facility (LMJ) at Bordeaux. We present two types of advanced monochromatic High Resolution X-ray Imaging microscopes (HRXI) who have high spatial resolution capability (3-6 μm) and high efficiency. The first microscope so-called MERSSIX consists of two toroïdals mirrors mounted into a Wolter type geometry and working at grazing incidence. Non-periodic multilayer (depth graded) mirrors were developed with special coatings designed to provide broadband X-ray reflectance in the 1 - 22 keV energy range. Associated to this Wolter microscope a potential monochromatic third mirror coated with a multilayer stack can be used for monochromatic application in that range. The second microscope is composed of a transmission gold Fresnel Phase Zone Plate (FPZP) and a narrow bandwidth multilayer mirror. We present an experimental study with X-ray plasma-source and a complete characterization of the X-ray optics on the synchrotron radiation facility BESSY II. Potentialities (a few μspatial resolution monochromatic images) and complementarity of these two monochromatic HRXI are discussed. The design of the MLs for each microscope is detailed.

  3. Ultra-high resolution water window x ray microscope optics design and analysis

    NASA Technical Reports Server (NTRS)

    Shealy, David L.; Wang, C.

    1993-01-01

    This project has been focused on the design and analysis of an ultra-high resolution water window soft-x-ray microscope. These activities have been accomplished by completing two tasks contained in the statement of work of this contract. The new results from this work confirm: (1) that in order to achieve resolutions greater than three times the wavelength of the incident radiation, it will be necessary to use spherical mirror surfaces and to use graded multilayer coatings on the secondary in order to accommodate the large variations of the angle of incidence over the secondary when operating the microscope at numerical apertures of 0.35 or greater; (2) that surface contour errors will have a significant effect on the optical performance of the microscope and must be controlled to a peak-to-valley variation of 50-100 A and a frequency of 8 periods over the surface of a mirror; and (3) that tolerance analysis of the spherical Schwarzschild microscope has been shown that the water window operations will require 2-3 times tighter tolerances to achieve a similar performance of operations with 130 A radiation. These results have been included in a manuscript included in the appendix.

  4. Measurement of ciliary beat frequency using ultra-high resolution optical coherence tomography

    NASA Astrophysics Data System (ADS)

    Chen, Jason J.; Jing, Joseph C.; Su, Erica; Badger, Christopher; Coughlan, Carolyn A.; Chen, Zhongping; Wong, Brian J. F.

    2016-02-01

    Ciliated epithelial cells populate up to 80% of the surface area of the human airway and are responsible for mucociliary transport, which is the key protective mechanism that provides the first line of defense in the respiratory tract. Cilia beat in a rhythmic pattern and may be easily affected by allergens, pollutants, and pathogens, altering ciliary beat frequency (CBF) subsequently. Diseases including cystic fibrosis, chronic obstructive pulmonary disease, and primary ciliary dyskinesia may also decrease CBF. CBF is therefore a critical component of respiratory health. The current clinical method of measuring CBF is phase-contrast microscopy, which involves a tissue biopsy obtained via brushing of the nasal cavity. While this method is minimally invasive, the tissue sample must be oriented to display its profile view, making the visualization of a single layer of cilia challenging. In addition, the conventional method requires subjective analysis of CBF, e.g., manually counting by visual inspection. On the contrary, optical coherence tomography (OCT) has been used to study the retina in ophthalmology as well as vasculature in cardiology, and offers higher resolution than conventional computed tomography and magnetic resonance imaging. Based on this technology, our lab specifically developed an ultra-high resolution OCT system to image the microstructure of the ciliated epithelial cells. Doppler analysis was also performed to determine CBF. Lastly, we also developed a program that utilizes fast Fourier transform to determine CBF under phase-contrast microscopy, providing a more objective method compared to the current method.

  5. High-resolution adaptive optics scanning laser ophthalmoscope with dual deformable mirrors for large aberration correction

    SciTech Connect

    Chen, D; Jones, S M; Silva, D A; Olivier, S S

    2007-01-25

    Scanning laser ophthalmoscopes with adaptive optics (AOSLO) have been shown previously to provide a noninvasive, cellular-scale view of the living human retina. However, the clinical utility of these systems has been limited by the available deformable mirror technology. In this paper, we demonstrate that the use of dual deformable mirrors can effectively compensate large aberrations in the human retina, making the AOSLO system a viable, non-invasive, high-resolution imaging tool for clinical diagnostics. We used a bimorph deformable mirror to correct low-order aberrations with relatively large amplitudes. The bimorph mirror is manufactured by Aoptix, Inc. with 37 elements and 18 {micro}m stroke in a 10 mm aperture. We used a MEMS deformable mirror to correct high-order aberrations with lower amplitudes. The MEMS mirror is manufactured by Boston Micromachine, Inc with 144 elements and 1.5 {micro}m stroke in a 3 mm aperture. We have achieved near diffraction-limited retina images using the dual deformable mirrors to correct large aberrations up to {+-} 3D of defocus and {+-} 3D of cylindrical aberrations with test subjects. This increases the range of spectacle corrections by the AO systems by a factor of 10, which is crucial for use in the clinical environment. This ability for large phase compensation can eliminate accurate refractive error fitting for the patients, which greatly improves the system ease of use and efficiency in the clinical environment.

  6. High-Resolution Optical Coherence Tomography Findings of Lisch Epithelial Corneal Dystrophy

    PubMed Central

    Pole, Cameron; Sise, Adam; Joag, Madhura; Galor, Anat; Bermudez-Magner, Jose Antonio; Dubovy, Sander; Karp, Carol L.

    2015-01-01

    Purpose To describe a case of Lisch epithelial corneal dystrophy (LECD) and present its unique characteristics on high-resolution optical coherence tomography (HR-OCT). Methods A 78-year-old man with whorled corneal epithelial opacities in the right eye was referred for evaluation of ocular surface squamous neoplasia. Clinical evaluation, photos, and HR-OCT images of involved cornea were obtained and scrapings of the affected cornea were sent for histopathologic analysis. Results Clinically the patient presented with a opalescent whirling epithelium in a linear pattern encroaching on the visual axis. HR-OCT showed normal thickness epithelial hyperreflectivity of involved cornea without stromal involvement, along with sharply demarcated borders of unaffected tissue. Histopathologic findings demonstrated vacuolated PAS-positive cells throughout the epithelial layers consistent with LECD. Conclusions HR-OCT was able to provide useful information to rule out ocular surface squamous neoplasia, and confirm the clinical impression of LECD at the time of clinical examination. HR-OCT shows promise as an adjunctive diagnostic tool for ocular surface lesions and pathologies. PMID:26764880

  7. A system for optical high resolution screening of electrical excitable cells.

    PubMed

    Müller, Oliver; Tian, Qinghai; Zantl, Roman; Kahl, Valentin; Lipp, Peter; Kaestner, Lars

    2010-03-01

    The application of primary excitable cells for high content screening (HCS) requires a multitude of novel developments including cell culture and multi-well plates. Here we introduce a novel system combining optimised culture conditions of primary adult cardiomyocytes with the particular needs of excitable cells for arbitrary field stimulation of individual wells. The major advancements of our design were tested in calcium imaging experiments and comprise (i) each well of the plate can be subjected to individual pulse protocols, (ii) the software driving electrical stimulation can run as a stand-alone application but also as a plug-in in HCS software packages, (iii) the optical properties of the plastic substrate (foil) resemble those of glass coverslips fostering high resolution immersion-based microscopy, (iv) the bottom of the foil is coated with an oleophobic layer that prevents immersion oil from sticking, (v) the top of the foil is coated with an elastic film. The latter enables cardiomyocytes to display loaded contractions by mimicking the physiologically occurring local elastic network (e.g. extracellular matrix) and results in significantly increased contractions (with identical calcium transients) when compared to non-elastic substrates. Thus, our novel design and culture conditions represent an essential further step towards the application of primary cultured adult cardiomyocytes for HCS applications.

  8. Wide-field and high-resolution optical imaging for early detection of oral neoplasia

    NASA Astrophysics Data System (ADS)

    Pierce, Mark C.; Schwarz, Richard A.; Rosbach, Kelsey; Roblyer, Darren; Muldoon, Tim; Williams, Michelle D.; El-Naggar, Adel K.; Gillenwater, Ann M.; Richards-Kortum, Rebecca

    2010-02-01

    Current procedures for oral cancer screening typically involve visual inspection of the entire tissue surface at risk under white light illumination. However, pre-cancerous lesions can be difficult to distinguish from many benign conditions when viewed under these conditions. We have developed wide-field (macroscopic) imaging system which additionally images in cross-polarized white light, narrowband reflectance, and fluorescence imaging modes to reduce specular glare, enhance vascular contrast, and detect disease-related alterations in tissue autofluorescence. We have also developed a portable system to enable high-resolution (microscopic) evaluation of cellular features within the oral mucosa in situ. This system is a wide-field epi-fluorescence microscope coupled to a 1 mm diameter, flexible fiber-optic imaging bundle. Proflavine solution was used to specifically label cell nuclei, enabling the characteristic differences in N/C ratio and nuclear distribution between normal, dysplastic, and cancerous oral mucosa to be quantified. This paper discusses the technical design and performance characteristics of these complementary imaging systems. We will also present data from ongoing clinical studies aimed at evaluating diagnostic performance of these systems for detection of oral neoplasia.

  9. Probing Protein Folding Kinetics with High-resolution, Stabilized Optical Tweezers

    NASA Astrophysics Data System (ADS)

    Wong, Wesley; Halvorsen, Ken

    2009-03-01

    Single-molecule techniques provide a powerful means of exploring molecular transitions such as the unfolding and refolding of a protein. However, the quantification of bi-directional transitions and near-equilibrium phenomena poses unique challenges, and is often limited by the detection resolution and long-term stability of the instrument. We have developed unique optical tweezers methods that address these problems, including an interference-based method for high-resolution 3D bead tracking (˜1 nm laterally, ˜0.3 nm vertically, at > 100 Hz), and a continuous autofocus system that stabilizes the trap height to within 1-2 nm longterm [1,2]. We have used our instruments to quantify the force-dependent unfolding and refolding kinetics of single protein domains (e.g. spectrin in collaboration with E. Evans). These single-molecule studies are presented, together with the accompanying probabilistic analysis that we have developed. References: 1. W.P. Wong, V. Heinrich, E. Evans, Mat. Res. Soc. Symp. Proc., 790, P5.1-P5.10 (2004). 2. V. Heinrich, W.P. Wong, K. Halvorsen, E. Evans, Langmuir, 24, 1194-1203 (2008).

  10. In vivo monitoring laser tissue interaction using high resolution Fourier-domain optical coherence tomography

    NASA Astrophysics Data System (ADS)

    Jo, Hang Chan; Shin, Dong Jun; Ahn, Jin-Chul; Chung, Phil-Sang; Kim, DaeYu

    2017-02-01

    Laser-induced therapies include laser ablation to remove or cut target tissue by irradiating high-power focused laser beam. These laser treatments are widely used tools for minimally invasive surgery and retinal surgical procedures in clinical settings. In this study, we demonstrate laser tissue interaction images of various sample tissues using high resolution Fourier-domain optical coherence tomography (Fd-OCT). We use a Q-switch diode-pumped Nd:YVO4 nanosecond laser (532nm central wavelength) with a 4W maximum output power at a 20 kHz repetition rate to ablate in vitro and in vivo samples including chicken breast and mouse ear tissues. The Fd-OCT system acquires time-series Bscan images at the same location during the tissue ablation experiments with 532nm laser irradiation. The real-time series of OCT cross-sectional (B-scan) images compare structural changes of 532nm laser ablation using same and different laser output powers. Laser tissue ablation is demonstrated by the width and the depth of the tissue ablation from the B-scan images.

  11. A targeted illumination optical fiber probe for high resolution fluorescence imaging and optical switching

    NASA Astrophysics Data System (ADS)

    Shinde, Anant; Perinchery, Sandeep Menon; Murukeshan, Vadakke Matham

    2017-04-01

    An optical imaging probe with targeted multispectral and spatiotemporal illumination features has applications in many diagnostic biomedical studies. However, these systems are mostly adapted in conventional microscopes, limiting their use for in vitro applications. We present a variable resolution imaging probe using a digital micromirror device (DMD) with an achievable maximum lateral resolution of 2.7 μm and an axial resolution of 5.5 μm, along with precise shape selective targeted illumination ability. We have demonstrated switching of different wavelengths to image multiple regions in the field of view. Moreover, the targeted illumination feature allows enhanced image contrast by time averaged imaging of selected regions with different optical exposure. The region specific multidirectional scanning feature of this probe has facilitated high speed targeted confocal imaging.

  12. A targeted illumination optical fiber probe for high resolution fluorescence imaging and optical switching.

    PubMed

    Shinde, Anant; Perinchery, Sandeep Menon; Murukeshan, Vadakke Matham

    2017-04-03

    An optical imaging probe with targeted multispectral and spatiotemporal illumination features has applications in many diagnostic biomedical studies. However, these systems are mostly adapted in conventional microscopes, limiting their use for in vitro applications. We present a variable resolution imaging probe using a digital micromirror device (DMD) with an achievable maximum lateral resolution of 2.7 μm and an axial resolution of 5.5 μm, along with precise shape selective targeted illumination ability. We have demonstrated switching of different wavelengths to image multiple regions in the field of view. Moreover, the targeted illumination feature allows enhanced image contrast by time averaged imaging of selected regions with different optical exposure. The region specific multidirectional scanning feature of this probe has facilitated high speed targeted confocal imaging.

  13. A targeted illumination optical fiber probe for high resolution fluorescence imaging and optical switching

    PubMed Central

    Shinde, Anant; Perinchery, Sandeep Menon; Murukeshan, Vadakke Matham

    2017-01-01

    An optical imaging probe with targeted multispectral and spatiotemporal illumination features has applications in many diagnostic biomedical studies. However, these systems are mostly adapted in conventional microscopes, limiting their use for in vitro applications. We present a variable resolution imaging probe using a digital micromirror device (DMD) with an achievable maximum lateral resolution of 2.7 μm and an axial resolution of 5.5 μm, along with precise shape selective targeted illumination ability. We have demonstrated switching of different wavelengths to image multiple regions in the field of view. Moreover, the targeted illumination feature allows enhanced image contrast by time averaged imaging of selected regions with different optical exposure. The region specific multidirectional scanning feature of this probe has facilitated high speed targeted confocal imaging. PMID:28368033

  14. Electrodynamics and energy characteristics of aurora at high resolution by optical methods

    NASA Astrophysics Data System (ADS)

    Dahlgren, H.; Lanchester, B. S.; Ivchenko, N.; Whiter, D. K.

    2016-06-01

    Technological advances leading to improved sensitivity of optical detectors have revealed that aurora contains a richness of dynamic and thin filamentary structures, but the source of the structured emissions is not fully understood. In addition, high-resolution radar data have indicated that thin auroral arcs can be correlated with highly varying and large electric fields, but the detailed picture of the electrodynamics of auroral filaments is yet incomplete. The Auroral Structure and Kinetics (ASK) instrument is a state-of-the-art ground-based instrument designed to investigate these smallest auroral features at very high spatial and temporal resolution, by using three electron multiplying CCDs in parallel for three different narrow spectral regions. ASK is specifically designed to utilize a new optical technique to determine the ionospheric electric fields. By imaging the long-lived O+ line at 732 nm, the plasma flow in the region can be traced, and since the plasma motion is controlled by the electric field, the field strength and direction can be estimated at unprecedented resolution. The method is a powerful tool to investigate the detailed electrodynamics and current systems around the thin auroral filaments. The two other ASK cameras provide information on the precipitation by imaging prompt emissions, and the emission brightness ratio of the two emissions, together with ion chemistry modeling, is used to give information on the energy and energy flux of the precipitating electrons. In this paper, we discuss these measuring techniques and give a few examples of how they are used to reveal the nature and source of fine-scale structuring in the aurora.

  15. Laminar optical tomography: high-resolution 3D functional imaging of superficial tissues

    NASA Astrophysics Data System (ADS)

    Hillman, Elizabeth M. C.; Devor, Anna; Dunn, Andrew K.; Boas, David A.

    2006-03-01

    Laminar Optical Tomography (LOT) is a new medical imaging modality for high-resolution, depth-resolved, functional imaging of superficial tissue such as rodent cortex, skin and the retina. LOT uses visible laser light to image to depths of >2mm (far deeper than microscopy) and is highly sensitive to absorption and fluorescence contrast, enabling spectroscopic functional information such as hemoglobin oxygenation to be imaged with 100-200 micron resolution. LOT has been used to image the hemodynamic response to stimulus in the somatosensory cortex of rats. The resulting three-dimensional (3D) images through the depth of the cortex can be used to delineate the arterial, capillary and venous responses, revealing new information about the intricacies of the oxygenation and blood flow dynamics related to neuronal activation. Additional applications of LOT are being explored, including the integration of 3D Voltage Sensitive Dye fluorescence imaging. LOT imaging uses a system similar to a confocal microscope, quickly scanning a focused beam of light over the surface of the tissue (~8Hz frame rate). Light is detected from both the focus of the scanning beam, and also at increasing distances from the beam's focus. This scattered light has penetrated more deeply into the tissue, and allows features at different depths to be distinguished. An algorithm that includes photon migration modeling of light scattering converts the raw data into 3D images. The motivation for functional optical imaging will be outlined, the basic principles of LOT imaging will be described, and the latest in-vivo results will be presented.

  16. High-resolution paleomagnetic records from Laguna Potrok Aike (Patagonia, Argentina) for the last 16,000 years

    NASA Astrophysics Data System (ADS)

    Gogorza, Claudia S. G.; Irurzun, MaríA. A.; Sinito, Ana M.; Lisé-Pronovost, Agathe; St-Onge, Guillaume; Haberzettl, Torsten; Ohlendorf, Christian; Kastner, Stephanie; Zolitschka, Bernd

    2012-02-01

    Holocene and Late-glacial records documenting variations in direction and intensity of the geomagnetic field during the last 16,000 cal. BP are presented for Southern Patagonia. This continuous high-resolution terrestrial record from Laguna Potrok Aike (51°58'S, 70°23'W) was recovered within the SALSA (South Argentinean Lake Sediment Archives and modeling) project. Mineral magnetic measurements indicate that pseudo single-domain magnetite is the major carrier of the remanence allowing the reliable determination of stable natural remanent magnetization inclinations and declinations from alternating field demagnetization and principal component analysis. Paleomagnetic secular variation records reveal most of the familiar features of declination and inclination that have previously been reported in other records from South Argentina but conspicuous centennial-scale differences are also observed. The results illustrate the potential of paleosecular variations records for dating sedimentary sequences in southern South America.

  17. High-resolution photography of clouds from the surface: Retrieval of optical depth of thin clouds down to centimeter scales: High-Resolution Photography of Clouds

    DOE PAGES

    Schwartz, Stephen E.; Huang, Dong; Vladutescu, Daniela Viviana

    2017-03-08

    This article describes the approach and presents initial results, for a period of several minutes in north central Oklahoma, of an examination of clouds by high resolution digital photography from the surface looking vertically upward. A commercially available camera having 35-mm equivalent focal length up to 1200 mm (nominal resolution as fine as 6 µrad, which corresponds to 9 mm for cloud height 1.5 km) is used to obtain a measure of zenith radiance of a 30 m × 30 m domain as a two-dimensional image consisting of 3456 × 3456 pixels (12 million pixels). Downwelling zenith radiance varies substantiallymore » within single images and between successive images obtained at 4-s intervals. Variation in zenith radiance found on scales down to about 10 cm is attributed to variation in cloud optical depth (COD). Attention here is directed primarily to optically thin clouds, COD less than about 2. A radiation transfer model used to relate downwelling zenith radiance to COD and to relate the counts in the camera image to zenith radiance, permits determination of COD on a pixel-by-pixel basis. COD for thin clouds determined in this way exhibits considerable variation, for example, an order of magnitude within 15 m, a factor of 2 within 4 m, and 25% (0.12 to 0.15) over 14 cm. In conclusion, this approach, which examines cloud structure on scales 3 to 5 orders of magnitude finer than satellite products, opens new avenues for examination of cloud structure and evolution.« less

  18. Optical micromachined ultrasound transducers (OMUT) - a new approach for high resolution imaging

    NASA Astrophysics Data System (ADS)

    Tadayon, M. A.; Ashkenazi, S.

    2013-03-01

    Piezoelectric ultrasound (US) transducers are at the heart of almost any ultrasonic medical imaging probe. However, their sensitivity and reliability severely degrade in applications requiring high frequency (>20 MHz) and small element size (<0.1 mm). Alternative technologies such as capacitive micromachined ultrasound transducers (CMUT) and optical sensing and generation of ultrasound are being investigated. In this paper we present our first steps in developing optical micromachined ultrasound transducers (OMUT) technology. OMUTs rely on microfabrication techniques to construct micron-size air cavities capped by an elastic membrane. The membrane functions as the active ultrasound transmitter and receiver. We will describe the design and testing of prototype OMUT devices which implement a receive-only function. The cavity detector is an optical cavity which its top mirror is deflected under the application of pressure. The intensity of a reflected light beam is highly sensitive to displacement of the top membrane if the optical wavelength is at near-resonance condition. Therefore, US pulses can be detected by recording the reflected light intensity. The sensitivity of the device depends on the mechanical properties of the top membrane and optical characteristics of the optical cavity. The device was fabricated using SU8 as a structural material and gold as a mirror. We have developed a new bonding method to fabricate a sealed, low roughness, high quality optical cavity. The 60μm cavity with the 8.5 μm top membrane is tested in water with 25MHz ultrasound transducer. The NEP of the device for bandwidth of 28MHz was 9.25kPa. The optical cavity has a finesse of around 23.

  19. Eolian and riverine contributions to central-Mediterranean sediments: a high-resolution Holocene record

    NASA Astrophysics Data System (ADS)

    Wu, Jiawang; Böning, Philipp; Pahnke, Katharina; Tachikawa, Kazuyo; De Lange, Gert

    2017-04-01

    Circum-Mediterranean climate variability is reflected in sediments deposited and preserved at the Mediterranean seafloor. Alternating depositions of organic-lean marls and organic-rich sapropel sediments in the eastern Mediterranean Sea (EMS) are clearly related to precessional hydroclimate variability. The exact origin for freshwater sources and related changes therein during sapropel formation are still debated. Here, Sr and Nd isotopes and high-resolution elemental ratios from core CP10BC are used to unravel and constrain different eolian and riverine supplies from North Africa and from northern borderlands to the central Mediterranean over the past 9.8 ka. Based on Sr and Nd isotopic and elemental compositions, the provenance for detrital sediments in the Levantine basin can be adequately described using 2-end-members. However, in the central Mediterranean, a three-endmember mixing system is required. The three endmember include Saharan Dust, Aegean/Nile, and Libyan Soil, which respectively represents the eolian supply from North Africa, the riverine inputs from the Aegean/Nile areas, and the riverine and shelf-derived fluxes from the Libyan-Tunisian margin. For the first time, robust and consistent evidence is given for important riverine supplies from the Libyan-Tunisian margin into the central Mediterranean during sapropel S1 time in particular. Considerable amounts of detrital materials and freshwater must have been delivered into the EMS through the fossil river/wadi systems, which were activated by intensified African monsoon precipitation. A west-east comparison of Sr-Nd isotope data between core CP10BC and 4 other cores throughout the EMS shows that, such detrital supplies originated mainly from western Libya and Tunisia, and were transported as far eastward as 25°E while being diluted by an increasing Nile contribution. The Nile contribution to the central-Mediterranean detrital sediment fraction appears to have been negligible. Moreover, elemental

  20. Motor protein and microtubule mechanics: Application of a novel high-resolution optical trapping technique

    NASA Astrophysics Data System (ADS)

    Allersma, Miriam W.

    Using optical tweezers and a novel detection technique (a quadrant photodiode at the back focal plane or, BFP-QD), this thesis investigates two problems in biophysics, ncd motility and microtubule flexural rigidity. We use optically trapped microspheres to probe the samples. The technique detects the displacements of the microspheres relative to the trap center by monitoring the laser intensity shifts in the back focal plane of the microscope condenser. We use a quadrant diode to detect the shifts, which are due to far-field interference between the trapping laser and scattered laser light from the trapped object. The method yields high-resolution (nm-spatial and μsec-temporal), two-dimensional data, which is largely independent of trap position in the field of view. We first studied the motility of ncd, a kinesin-related motor protein. Motor proteins are able to harness the energy of ATP hydrolysis to perform mechanical work for the cell. Many ncd molecules were adsorbed onto silica microspheres and their motions along the microtubule surface lattice were observed with the BFP-QD method. Since the method is two-dimensional, we were able to monitor axial and lateral motions simultaneously. The average axial velocity was 230 +/- 30 nm/sec (average +/- SD). The high temporal resolution allowed us to investigate dynamical parameters. Spectral analysis showed an increase in viscous drag near the surface for ncd-driven microspheres. In addition, we found that the binding of the motors to microtubules in the presence of the nonhydrolyzable nucleotide adenylylimidodiphosphate caused an increase in the motor elastic constraint. Using a dual optical trap configuration in conjunction with the BFP-QD, we also investigated the elastic properties of taxol-stabilized microtubules. Cytoskeletal filaments are responsible for myriad structural cell functions. Our results were not readily interpreted by a standard bent strut treatment because of the finite size of the microspheres

  1. Fast, high-resolution 3D dosimetry utilizing a novel optical-CT scanner incorporating tertiary telecentric collimation

    SciTech Connect

    Sakhalkar, H. S.; Oldham, M.

    2008-01-15

    This study introduces a charge coupled device (CCD) area detector based optical-computed tomography (optical-CT) scanner for comprehensive verification of radiation dose distributions recorded in nonscattering radiochromic dosimeters. Defining characteristics include: (i) a very fast scanning time of {approx}5 min to acquire a complete three-dimensional (3D) dataset, (ii) improved image formation through the use of custom telecentric optics, which ensures accurate projection images and minimizes artifacts from scattered and stray-light sources, and (iii) high resolution (potentially 50 {mu}m) isotropic 3D dose readout. The performance of the CCD scanner for 3D dose readout was evaluated by comparison with independent 3D readout from the single laser beam OCTOPUS-scanner for the same PRESAGE dosimeters. The OCTOPUS scanner was considered the 'gold standard' technique in light of prior studies demonstrating its accuracy. Additional comparisons were made against calculated dose distributions from the ECLIPSE treatment-planning system. Dose readout for the following treatments were investigated: (i) a single rectangular beam irradiation to investigate small field and very steep dose gradient dosimetry away from edge effects, (ii) a 2-field open beam parallel-opposed irradiation to investigate dosimetry along steep dose gradients, and (iii) a 7-field intensity modulated radiation therapy (IMRT) irradiation to investigate dosimetry for complex treatment delivery involving modulation of fluence and for dosimetry along moderate dose gradients. Dose profiles, dose-difference plots, and gamma maps were employed to evaluate quantitative estimates of agreement between independently measured and calculated dose distributions. Results indicated that dose readout from the CCD scanner was in agreement with independent gold-standard readout from the OCTOPUS-scanner as well as the calculated ECLIPSE dose distribution for all treatments, except in regions within a few millimeters of

  2. Fast, high-resolution 3D dosimetry utilizing a novel optical-CT scanner incorporating tertiary telecentric collimation

    PubMed Central

    Sakhalkar, H. S.; Oldham, M.

    2008-01-01

    This study introduces a charge coupled device (CCD) area detector based optical-computed tomography (optical-CT) scanner for comprehensive verification of radiation dose distributions recorded in nonscattering radiochromic dosimeters. Defining characteristics include: (i) a very fast scanning time of ~5 min to acquire a complete three-dimensional (3D) dataset, (ii) improved image formation through the use of custom telecentric optics, which ensures accurate projection images and minimizes artifacts from scattered and stray-light sources, and (iii) high resolution (potentially 50 μm) isotropic 3D dose readout. The performance of the CCD scanner for 3D dose readout was evaluated by comparison with independent 3D readout from the single laser beam OCTOPUS™-scanner for the same PRESAGE™ dosimeters. The OCTOPUS™ scanner was considered the “gold standard” technique in light of prior studies demonstrating its accuracy. Additional comparisons were made against calculated dose distributions from the ECLIPSE treatment-planning system. Dose readout for the following treatments were investigated: (i) a single rectangular beam irradiation to investigate small field and very steep dose gradient dosimetry away from edge effects, (ii) a 2-field open beam parallel-opposed irradiation to investigate dosimetry along steep dose gradients, and (iii) a 7-field intensity modulated radiation therapy (IMRT) irradiation to investigate dosimetry for complex treatment delivery involving modulation of fluence and for dosimetry along moderate dose gradients. Dose profiles, dose-difference plots, and gamma maps were employed to evaluate quantitative estimates of agreement between independently measured and calculated dose distributions. Results indicated that dose readout from the CCD scanner was in agreement with independent gold-standard readout from the OCTOPUS™-scanner as well as the calculated ECLIPSE dose distribution for all treatments, except in regions within a few

  3. Fast, high-resolution 3D dosimetry utilizing a novel optical-CT scanner incorporating tertiary telecentric collimation.

    PubMed

    Sakhalkar, H S; Oldham, M

    2008-01-01

    This study introduces a charge coupled device (CCD) area detector based optical-computed tomography (optical-CT) scanner for comprehensive verification of radiation dose distributions recorded in nonscattering radiochromic dosimeters. Defining characteristics include: (i) a very fast scanning time of approximately 5 min to acquire a complete three-dimensional (3D) dataset, (ii) improved image formation through the use of custom telecentric optics, which ensures accurate projection images and minimizes artifacts from scattered and stray-light sources, and (iii) high resolution (potentially 50 microm) isotropic 3D dose readout. The performance of the CCD scanner for 3D dose readout was evaluated by comparison with independent 3D readout from the single laser beam OCTOPUS-scanner for the same PRESAGE dosimeters. The OCTOPUS scanner was considered the "gold standard" technique in light of prior studies demonstrating its accuracy. Additional comparisons were made against calculated dose distributions from the ECLIPSE treatment-planning system. Dose readout for the following treatments were investigated: (i) a single rectangular beam irradiation to investigate small field and very steep dose gradient dosimetry away from edge effects, (ii) a 2-field open beam parallel-opposed irradiation to investigate dosimetry along steep dose gradients, and (iii) a 7-field intensity modulated radiation therapy (IMRT) irradiation to investigate dosimetry for complex treatment delivery involving modulation of fluence and for dosimetry along moderate dose gradients. Dose profiles, dose-difference plots, and gamma maps were employed to evaluate quantitative estimates of agreement between independently measured and calculated dose distributions. Results indicated that dose readout from the CCD scanner was in agreement with independent gold-standard readout from the OCTOPUS-scanner as well as the calculated ECLIPSE dose distribution for all treatments, except in regions within a few

  4. Vertical and horizontal corneal epithelial thickness profiles determined by ultra-high resolution optical coherence tomography

    PubMed Central

    Du, Chixin; Wang, Jianhua; Cui, Lele; Shen, Meixiao; Yuan, Yimin

    2011-01-01

    Purpose To measure vertical and horizontal thickness profiles of the central and peripheral corneal epithelium and determine if daytime changes occur. Methods Forty eyes of 20 normal subjects were imaged by ultra-high resolution spectral domain optical coherence tomography to profile the corneal epithelial thickness from the edge of Bowman’s layer to the central cornea across the vertical and horizontal meridians. Measurements were made at 10:00 AM and again at 6, 8 hours later. Results The baseline vertical meridional epithelial thickness was thinnest, 42.9±4.1 μm, at the edge of Bowman’s layer in the superior region. It increased in thickness (p<0.01), towards the central cornea. The central epithelium averaged 52.5±2.4 μm, becoming thickest, 55.2±2.5 μm, in the inferior pericentral region. It thinned towards the inferior periphery, reaching 51.3±5.1 μm at the edge of Bowman’s layer (p<0.01). Along the horizontal meridian, the epithelium was thickest at the nasal side, 58.6±5.1 μm, and temporal side, 59.3±6.6 μm, near the edges of Bowman’s layer. It thinned towards the central cornea. There were no significant changes in the epithelial thickness at any location over 8 hours. Conclusion Epithelial thickness varied over the horizontal and vertical meridians and appeared stable during the daytime. PMID:22357393

  5. Very High Resolution Solar X-ray Imaging Using Diffractive Optics

    NASA Technical Reports Server (NTRS)

    Dennis, B. R.; Skinner, G. K.; Li, M. J.; Shih, A. Y.

    2012-01-01

    This paper describes the development of X-ray diffractive optics for imaging solar flares with better than 0.1 arcsec angular resolution. X-ray images with this resolution of the greater than or equal to 10 MK plasma in solar active regions and solar flares would allow the cross-sectional area of magnetic loops to be resolved and the coronal flare energy release region itself to be probed. The objective of this work is to obtain X-ray images in the iron-line complex at 6.7 keV observed during solar flares with an angular resolution as fine as 0.1 arcsec - over an order of magnitude finer than is now possible. This line emission is from highly ionized iron atoms, primarily Fe xxv, in the hottest flare plasma at temperatures in excess of approximately equal to 10 MK. It provides information on the flare morphology, the iron abundance, and the distribution of the hot plasma. Studying how this plasma is heated to such high temperatures in such short times during solar flares is of critical importance in understanding these powerful transient events, one of the major objectives of solar physics.We describe the design, fabrication, and testing of phase zone plate X-ray lenses with focal lengths of approximately equal to 100 m at these energies that would be capable of achieving these objectives. We show how such lenses could be included on a two-spacecraft formation-flying mission with the lenses on the spacecraft closest to the Sun and an X-ray imaging array on the second spacecraft in the focal plane approximately equal to 100 m away. High resolution X-ray images could be obtained when the two spacecraft are aligned with the region of interest on the Sun. Requirements and constraints for the control of the two spacecraft are discussed together with the overall feasibility of such a formation-flying mission.

  6. Abundance Analysis of 17 Planetary Nebulae from High-Resolution Optical Spectroscopy

    NASA Astrophysics Data System (ADS)

    Sherrard, Cameroun G.; Sterling, Nicholas C.; Dinerstein, Harriet L.; Madonna, Simone; Mashburn, Amanda

    2017-06-01

    We present an abundance analysis of 17 planetary nebulae (PNe) observed with the 2D-coudé echelle spectrograph on the 2.7-m Harlan J. Smith telescope at McDonald Observatory. The spectra cover the wavelength range 3600--10,400 Å at a resolution R = 36,700, and are the first high-resolution optical spectra for many objects in our sample. The number of emission lines detected in individual nebulae range from ~125 to over 600. We derive temperatures, densities, and abundances from collisionally-excited lines using the PyNeb package (Luridiana et al. 2015, A&A, 573, A42) and the ionization correction factor scheme of Delgado-Inglada et al. (2014, MNRAS, 440, 536). The abundances of light elements agree with previous estimates for most of the PNe. Several objects exhibit emission lines of refractory elements such as K and Fe, and neutron-capture elements that can be enriched by the s-process. We find that K and Fe are depleted relative to solar by ~0.3--0.7~dex and 1-2 dex, respectively, and find evidence for s-process enrichments in 10 objects. Several objects in our sample exhibit C, N, and O recombination lines that are useful for abundance determinations. These transitions are used to compute abundance discrepancy factors (ADFs), the ratio of ionic abundances derived from permitted lines to those from collisionally-excited transitions. We explore relations among depletion factors, ADFs, s-process enrichment factors, and other nebular stellar and nebular properties. We acknowledge support from NSF awards AST-901432 and AST-0708429.

  7. Deriving High Resolution UV Aerosol Optical Depth over East Asia using CAI-OMI Joint Retrieval

    NASA Astrophysics Data System (ADS)

    Go, S.; Kim, J.; KIM, M.; Lee, S.

    2015-12-01

    Monitoring aerosols using near UV spectral region have been successfully performed over decades by Ozong Monitoring Instruments (OMI) with benefit of strong aerosol signal over continuous dark surface reflectance, both land and ocean. However, because of big foot print of OMI, the cloud contamination error was a big issue in the UV aerosol algorithm. In the present study, high resolution UV aerosol optical depth (AOD) over East Asia was derived by collaborating the Greenhouse gases Observing SATellite/Thermal And Near infrared Sensor for carbon Observation (GOSAT/TANSO)-Cloud and Aerosol Imager (CAI) and OMI together. AOD of 0.1 degree grid resolution was retrieved using CAI band 1 (380nm) by bring OMI lv.2 aerosol type, single scattering albedo, and aerosol layer peak height in 1 degree grid resolution. Collocation of the two dataset within the 0.5 degree grid with time difference of OMI and CAI less than 5 minute was selected. Selected region becomes wider as it goes to the higher latitude. Also, calculated degradation factor of 1.57 was applied to CAI band1 (380nm) by comparing normalized radiance and Lambertian Equivalent Reflectivity (LER) of both sensors. The calculated degradation factor was reasonable over dark scene, but inconsistent over cirrus cloud and bright area. Then, surface reflectance was developed by compositing CAI LER minimum data over three month period, since the infrequent sampling rate associated with the three-day recursion period of GOSAT and the narrow CAI swath of 1000 km. To retrieve AOD, look up table (LUT) was generated using radiative transfer model VLIDORT NGST. Finally, the retrieved AOD was validated with AERONET ground based measurement data during the Dragon-NE Asia campaign in 2012.

  8. High resolution late Pliocene sea-surface temperature record from the northeast Atlantic Ocean

    USGS Publications Warehouse

    Dowsett, H.J.; Loubere, P.

    1992-01-01

    Application of quantitative micropaleontologic methods of sea-surface temperature (SST) estimation to the planktic foraminiferal faunas at Hole 548 in the northeast Atlantic between 3.5 and 2.0 Ma provide a detailed record of Pliocene surface temperature changes during the onset of major Northern Hemisphere glaciation. SST estimates indicate a relatively warm interval of "enhanced interglacials (warm peaks)" between 3.2 and 2.8 Ma followed by steady cooling with a significant step-like change toward colder temperatures occurring about 2.4 Ma. This record compares well with faunal and SST records from other North Atlantic sites. ?? 1992.

  9. High resolution spectroscopy of the young eruptive star V2492 Cyg currently peaking at record brightness

    NASA Astrophysics Data System (ADS)

    Munari, U.; Traven, G.; Dallaporta, S.; Lorenzetti, D.; Giannini, T.; Antoniucci, S.

    2017-03-01

    Recently, Ibryamov and Semkov (ATel #10170) have reported on a new record brightness attended by the young eruptive star V2492 Cyg along the complex outburst it started in 2010 (Itagaki CBET #2426, Munari et al. CBET #2428).

  10. Investigation of solar active regions at high resolution by balloon flights of the solar optical universal polarimeter, extended definition phase

    NASA Technical Reports Server (NTRS)

    Tarbell, Theodore D.

    1993-01-01

    Technical studies of the feasibility of balloon flights of the former Spacelab instrument, the Solar Optical Universal Polarimeter, with a modern charge-coupled device (CCD) camera, to study the structure and evolution of solar active regions at high resolution, are reviewed. In particular, different CCD cameras were used at ground-based solar observatories with the SOUP filter, to evaluate their performance and collect high resolution images. High resolution movies of the photosphere and chromosphere were successfully obtained using four different CCD cameras. Some of this data was collected in coordinated observations with the Yohkoh satellite during May-July, 1992, and they are being analyzed scientifically along with simultaneous X-ray observations.

  11. High-resolution 900 year volcanic and climatic record from the Vostok area, East Antarctica

    NASA Astrophysics Data System (ADS)

    Osipov, E. Y.; Khodzher, T. V.; Golobokova, L. P.; Onischuk, N. A.; Lipenkov, V. Y.; Ekaykin, A. A.; Shibaev, Y. A.; Osipova, O. P.

    2014-05-01

    Ion chromatography measurements of 1730 snow and firn samples obtained from three short cores and one pit in the Vostok station area, East Antarctica, allowed for the production of the combined volcanic record of the last 900 years (AD 1093-2010). The resolution of the record is 2-3 samples per accumulation year. In total, 24 volcanic events have been identified, including seven well-known low-latitude eruptions (Pinatubo 1991, Agung 1963, Krakatoa 1883, Tambora 1815, Huanaputina 1600, Kuwae 1452, El Chichon 1259) found in most of the polar ice cores. In comparison with three other East Antarctic volcanic records (South Pole, Plateau Remote and Dome C), the Vostok record contains more events within the last 900 years. The differences between the records may be explained by local glaciological conditions, volcanic detection methodology, and, probably, differences in atmospheric circulation patterns. The strongest volcanic signal (both in sulfate concentration and flux) was attributed to the AD 1452 Kuwae eruption, similar to the Plateau Remote and Talos Dome records. The average snow accumulation rate calculated between volcanic stratigraphic horizons for the period AD 1260-2010 is 20.9 mm H2O. Positive (+13%) anomalies of snow accumulation were found for AD 1661-1815 and AD 1992-2010, and negative (-12%) for AD 1260-1601. We hypothesized that the changes in snow accumulation are associated with regional peculiarities in atmospheric transport.

  12. High-resolution Holocene South American monsoon history recorded by a speleothem from Botuverá Cave, Brazil

    NASA Astrophysics Data System (ADS)

    Bernal, J. P.; Cruz, Francisco W.; Stríkis, Nicolás M.; Wang, Xianfeng; Deininger, Michael; Catunda, Maria Carolina A.; Ortega-Obregón, C.; Cheng, Hai; Edwards, R. Lawrence; Auler, Augusto S.

    2016-09-01

    A Holocene stalagmite from Botuverá Cave, southeastern Brazil was analyzed by LA-ICPMS for Mg/Ca, Sr/Ca, Ba/Ca. The observed variability in the record was demonstrated to be modulated by prior calcite precipitation, and, thus, is interpreted to reflect monsoon intensity. We find that the calcite δ18O is strongly correlated with Sr/Ca, indicating that atmospheric circulation over South America and monsoon intensity have been tightly correlated throughout most of the Holocene, both directly responding to solar precession. Comparison with other contemporaneous high-resolution hydroclimate records reveals that SAMS has shown a degree of complexity during the Holocene not previously detected, with periods where the South American Convergence Zone (SACZ) expanded to cover most of the South American sub-continent, and coincident with periods of low-SST in the north Atlantic. We also detect periods where rainfall amount in northeastern and southeastern Brazil are markedly anti-phased, suggesting a north-south migration of SACZ, which it appears to be mediated by solar irradiance. The high-resolution nature of our record allow us to examine the effect that Holocene climate anomalies had upon SAMS dynamics and hydroclimate in southeastern Brazil, in particular the 8.2 ka event and the Little Ice Age. In addition to confirm the internal structure of the events, we also detect the possible consequences of the climatic anomalies upon ocean-atmosphere interactions through its effects upon SAMS.

  13. The key technologies research on the large field-of-view and high-resolution optical synthesis telescope

    NASA Astrophysics Data System (ADS)

    Wang, Haitao; Luo, Qiufeng; Zhu, Yongkai; Ma, Wantai; Zhang, Yajing; Tian, Gui Yun

    2010-08-01

    It briefly introduces the international development status of the high resolution for air-to-ground remote sensing satellite. High resolution for the air-to-ground observation is also the civil and martial pursuing target. Because of the rising cost along with the large-diameter telescope, the weight, cubage will also become large. Nowadays, how to get high resolution with light weight, small cubage launch and large diameter is one of the important research directions in many countries. We raise a method of large field-of-view and high resolution optical synthesis telescope which can solve this problem. It is a co-phased segment mirrors which synthetic aperture diameter is about 1 m. Four 50cm diameter segment mirrors can fulfill the requirement. It is folded during its launch and is spread after it reaches to its working spot. In this way, it can reach the requirement of low launch weight, small launch cubage and can get high resolution observation. This method contains the key technologies of real-time UV coverage, optics design optimization, co-phase measurement and adjustment, micro-displacement sensor technology, the optics design and structure design. We explore the technology which can fulfill field-of-view of 1.86° and the resolution of 0.4m. We will discuss the UV-coverage method which includes the aperture arrangement, the relationship between the aperture number and the synthetic aperture diameter. There are much more detail calculation and analysis to it. Something is discussed about its structure design and optics design in the paper.

  14. The key technologies research on the large field-of-view and high resolution optical synthesis telescope

    NASA Astrophysics Data System (ADS)

    Wang, Haitao; Zhu, Yongkai; Ma, Wantai; Cai, Jiahui; Zhang, Yajing; Tian, Gui Yun

    2008-08-01

    It briefly introduces the international development status of the high resolution for air-to-ground remote sensing satellite. High resolution for the air-to-ground observation is also the civil and martial pursuing target. Because of the rising cost along with the large-diameter telescope, the weight, cubage will also become large. Nowadays, how to get high resolution with light weight, small cubage launch and large diameter is one of the important research directions in many countries. We raise a method of large field-of-view and high resolution optical synthesis telescope which can solve this problem. It is a co-phased segment mirrors which synthetic aperture diameter is about 1 m. Four 50cm diameter segment mirrors can fulfill the requirement. It is folded during its launch and is spread after it reaches to its working spot. In this way, it can reach the requirement of low launch weight, small launch cubage and can get high resolution observation. This method contains the key technologies of real-time UV coverage, optics design optimization, co-phase measurement and adjustment, micro-displacement sensor technology, the optics design and structure design. We explore the technology which can fulfill field-of-view of 1.86° and the resolution of 0.4m. We will discuss the UV-coverage method which includes the aperture arrangement, the relationship between the aperture number and the synthetic aperture diameter. There are much more detail calculation and analysis to it. Something is discussed about its structure design and optics design in the paper.

  15. Twentieth century sea-ice trends in the Ross Sea from a high-resolution, coastal ice-core record

    NASA Astrophysics Data System (ADS)

    Sinclair, Kate E.; Bertler, Nancy A. N.; Bowen, Melissa M.; Arrigo, Kevin R.

    2014-05-01

    We present the first proxy record of sea-ice area (SIA) in the Ross Sea, Antarctica, from a 130 year coastal ice-core record. High-resolution deuterium excess data show prevailing stable SIA from the 1880s until the 1950s, a 2-5% reduction from the mid-1950s to the early-1990s, and a 5% increase after 1993. Additional support for this reconstruction is derived from ice-core methanesulphonic acid concentrations and whaling records. While SIA has continued to decline around much of the West Antarctic coastline since the 1950s, concurrent with increasing air and ocean temperatures, the underlying trend is masked in the Ross Sea by a switch to positive SIA anomalies since the early-1990s. This increase is associated with a strengthening of southerly winds and the enhanced northward advection of sea ice.

  16. High Resolution Record of Carbon Accumulation Rates During Boreal Peatland Initiation

    NASA Astrophysics Data System (ADS)

    Chmura, G. L.; Pendea, I.

    2011-12-01

    Boreal peatlands have important feedbacks to climate as a major global carbon sink but also through release of CH4 to the atmosphere. Paleoenvironmental studies reveal variations in peatland C accumulation rates over the Holocene that have been linked with a decreased concentration in atmospheric CO2 7,000-10,000 years ago. This increased carbon storage has been attributed to climate change and increased productivity. Yet, this period also would have corresponded to early stages of peatland development following retreat of ice sheets and substantial increases in storage could be associated with wetland evolution via lake filling or following marine shoreline emergence. Unravelling past links amongst peatland dynamics, carbon storage, and climate will help us assess potential feedbacks form future changes in these systems, but most paleo studies of boreal peatlands are hampered by low temporal resolution associated with compaction and limitations of 14C dating. Decadal scale C accumulation rates can be determined by dating surface deposits with the short lived radioisotopes 210Pb and 137Cs. However, determination of high resolution C accumulation rates associated with early peatland evolution requires location of a fen that has transformed from salt marsh during a period within the limitation of 210Pb-dating, the last 100-200 yr, a condition not readily visible on the landscape. Boreal coastal regions presently undergoing isostatic rebound can provide such analogues and we located a site in early stages of peatland formation on the coast of James Bay, Quebec. We used palynological analyses to reconstruct ecological changes, measured sediment organic C density, and dated the deposits with 210Pb and 137Cs, and thus were able to document C accumulation rates at a sub-decadal scale. The site transitioned from bay to high elevation tidal marsh to a fen in about 70 yr, a phase difficult to bracket with 14C dating. The high carbon accumulation rates during the tidal marsh

  17. High-resolution frequency-domain second-harmonic optical coherence tomography

    NASA Astrophysics Data System (ADS)

    Su, Jianping; Tomov, Ivan V.; Jiang, Yi; Chen, Zhongping

    2007-04-01

    We used continuum generated in an 8.5 cm long fiber by a femtosecond Yb fiber laser to improve threefold the axial resolution of frequency domain second-harmonic optical coherence tomography (SH-OCT) to 12 μm. The acquisition time was shortened by more than 2 orders of magnitude compared to the time-domain SH-OCT. The system was applied to image biological tissue of fish scales, pig leg tendon, and rabbit eye sclera. Highly organized collagen fibrils can be visualized in the recorded images. Polarization dependence on the SH has been used to obtain polarization resolved images.

  18. Verlorenvlei - The first continuous Holocene high-resolution lake sediment record from the Winter Rainfall Zone of South Africa

    NASA Astrophysics Data System (ADS)

    Haberzettl, T.; Kasper, T.; Lederer, M.; Wündsch, M.; Frenzel, P.; Zabel, M.; Kirsten, K. L.; Meadows, M. E.; Quick, L. J.; St-Onge, G.; Maeusbacher, R.

    2015-12-01

    Verlorenvlei is a coastal lake in the Winter Rainfall Zone of the Western Cape Province of South Africa. Up to now several attempts have been made to recover sediment cores from this lake. However, no continuous high-resolution record covering large parts of the Holocene has been available so far. Within the project RAIN (Regional Archives for Integrated iNvestigations) it was possible to recover a 14.2 m paired parallel core from the central part of Verlorenvlei. Investigations on recent surface sediment distributions (elemental composition and grain sizes) indicate that this sediment core is very well suited for paleoenvironmental reconstructions. Using a set of 23 radiocarbon ages, a chronology for the past 9,000 cal BP was established which suggests continuous sedimentation over this period. Preliminary lithological and geochemical investigations show that this record can be used for sea level reconstructions as the lake was periodically inundated by the ocean during the past 9,000 cal BP. This is recorded in distinctly elevated Ca and Sr contents as well as the occurrence of marine indicator species (snail and mussel shells) in parts of the sediment core. Thin, pale grey layers of fine sediment occurring at various sediment depths seem to reflect event related deposits. In terms of lithology, geochemical and magnetic composition, the upper 50 cm clearly differ from the rest of the record and indicate increased sediment supply from the catchment, which is likely linked to anthropogenic farming activities. In conclusion, the newly recovered sediment record from Verlorenvlei offers excellent potential for a detailed, high-resolution reconstruction of sea level changes, climate variations and anthropogenic impact during the past 9,000 cal BP in an area in which natural archives are very scarce or poorly dated.

  19. A High-Resolution Upper Pleistocene Palaeomagnetic Record from the Fronte Section at Taranto, Italy

    NASA Astrophysics Data System (ADS)

    Lurcock, P. C.; Florindo, F.; Negri, A.; Marabini, S.; Mastronuzzi, G.; Morigi, C.; Vai, G. B.

    2015-12-01

    As part of a project to establish a global boundary stratotype section and point (GSSP) for the Upper Pleistocene, we have investigated the geomagnetic palaeosecular variation and relative palaeointensity of two parallel marine sedimentary cores drilled at the Fronte section at Taranto, Italy. U-channel samples were taken wherever core recovery and lithology permitted, producing a palaeomagnetic record at 1 cm resolution covering around 5 m of the total 9 m coring depth; discrete samples were taken from several of the intervals unsuitable for u-channelling.Stepwise alternating-field treatment resulted in good demagnetization behaviour, with most samples showing a clear, major origin-directed magnetization component. The average inclination of around 57° is close to the geocentric axial dipole inclination of 60° for the sampling site, with the difference attributable to typical inclination shallowing effects.Our record contains several brief excursions of shallowed inclination. The most significant of these reaches inclinations of below 10°, and we have tentatively correlated it with the Blake event. Relative palaeointensity exhibits a similarly dynamic behaviour across this interval. Integration of our palaeomagnetic record with ongoing biostratigraphic work, and with other regional palaeomagnetic data, will provide a more thorough characterization of Late Pleistocene palaeosecular variation in southern Europe.

  20. High-resolution patterning electronic polymers using dopant induced solubility control (Presentation Recording)

    NASA Astrophysics Data System (ADS)

    Moule, Adam J.; Jacobs, Ian E.; Li, Jun; Burg, Stephanie L.; Bilsky, David J.; Rotondo, Brandon T.; Stroeve, Pieter

    2015-08-01

    Organic electronics promise to provide flexible, large-area circuitry such as photovoltaics, displays, and light emitting diodes that can be fabricated inexpensively from solutions. A major obstacle to this vision is that most conjugated organic materials are miscible, making solution-based fabrication of multilayer or micro- to nanoscale patterned films problematic. Here we demonstrate that the solubility of prototypical conductive polymer poly(3-hexylthiophene) (P3HT) can be reversibly "switched off" using high electron affinity molecular dopants, then later recovered with light or a suitable dedoping solution. Using this technique, we are able to stack mutually soluble materials and laterally pattern polymer films using evaporation of dopants through a shadow mask or with light, achieving sub-micrometer, optically limited feature sizes. After forming these structures, the films can be dedoped without disrupting the patterned features; dedoped films have identical optical characteristics, charge carrier mobilities, and NMR spectra as as-cast P3HT films. This method greatly simplifies solution-based device fabrication, is easily adaptable to current manufacturing workflows, and is potentially generalizable to other classes of materials.

  1. Observation and Analysis of High Resolution Optical Line Profiles in Comet Hyakutake (C/1996 B2)

    NASA Astrophysics Data System (ADS)

    Combi, M. R.; Cochran, A. L.

    1997-07-01

    Very high resolution (R=200,000) and high signal-to-noise echelle spectra were obtained of comet C/Hyakutake 1996 B2 using the 2DCoude spectrograph on the 2.7 m telescope at McDonald Observatory during late March and early April 1996. Doppler resolved profiles are presented for individual lines of most of the major optical neutral species: CN, C_2, O((1) D) at 6300{ Angstroms}, O((1) S) at 5577{ Angstroms} , NH_2, and H Balmer-alpha at 6563{ Angstroms}. These may be the first ever to be published for CN, C_2, and O((1) S). In all cases the instrument spectral function is smaller than the intrinsic line widths of the individual cometary lines, so the observations provide clear signatures of lines which are Doppler broadened by different combinations of the coma expansion, exothermic photochemical ejection speeds, and collisional thermalization. For modeling analysis of these data we have used a hybrid fluid/kinetic Monte Carlo approach which can realistically include all of the relevant physical/chemical processes important for shaping the spectral lines. Because of the very short lifetime of the NH_2 parent (NH_3), the NH_2 is collisionally thermalized and provides an excellent probe of the outflow of the expanding coma. Because O((1) D) atoms in the region sampled are produced mainly by the photodissociation of water and the resulting photon is a prompt emission, the line retains signatures of both the basic coma expansion velocity and the 1.6 km s(-1) ejection speed of the O({(1}) D) atoms. The O((1) S) profile is consistent with that for the O((1) D). The profiles of CN and C_2 are somewhat broadened (CN more so than C_2), compared with NH_2, and seem to require a combination of coma expansion and the exothermic ejection speed they receive upon their production. Although the H Balmer-alpha line is complicated by a chance coincidence of an H_2O(+) line and optical depth effects in the solar Lyman-beta which pumps the Balmer-alpha emission, the spread of the wings

  2. High-resolution optical spectroscopy with a buffer-gas-cooled beam of BaH molecules

    NASA Astrophysics Data System (ADS)

    Iwata, G. Z.; McNally, R. L.; Zelevinsky, T.

    2017-08-01

    Barium monohydride (BaH) is an attractive candidate for extending laser cooling and trapping techniques to diatomic hydrides. The apparatus and high-resolution optical spectroscopy presented here demonstrate progress toward this goal. A cryogenic buffer-gas-cooled molecular beam of BaH was constructed and characterized. Pulsed laser ablation into cryogenic helium buffer gas delivers ˜1 ×1010 molecules/sr/pulse in the X +2Σ (v''=0 ,N''=1 ) state of primary interest. More than 1 ×107 of these molecules per pulse enter the downstream science region with forward velocities below 100 m/s and transverse temperature of 0.1 K. This molecular beam enabled high-resolution optical spectra of BaH in quantum states relevant to laser slowing and cooling. The reported measurements include hyperfine structure and magnetic g factors in the X +2Σ , B +2Σ , and A 1/2 2Π states.

  3. Systematic recover of long high-resolution rainfall time series recorded by pluviographs during the 20th century.

    NASA Astrophysics Data System (ADS)

    Delitala, Alessandro M. S.; Deidda, Roberto; Mascaro, Giuseppe; Piga, Enrico; Querzoli, Giorgio

    2010-05-01

    During most of the 20th century, precipitation has been continuously measured by means of the so-called "pluviographs", i.e. rain gauges including a mechanical apparatus for continuously recording the depth of water from precipitation on specific strip charts, usually on a weekly basis. The signal recorded on such strips was visually examined by trained personnel on a regular basis, in order to extract the daily precipitation totals and the maximum precipitation intensities over short periods (from a few minutes to hours). The rest of the high-resolution information contained in the signal was usually not extracted, except for specific cases. A systematic recovering of the entire information at high temporal resolution contained in these precipitation signals would provide a fundamental database to improve the characterization of historical rainfall climatology during the previous century. The Department of Land Engineering of the University of Cagliari has recently developed and tested an automatic software, based on image analysis techniques, which is able to acquire the scanned images of the pluviograph strip charts, to automatically digitise the signal and to produce a digital database of continuous precipitation records at the highest possible temporal resolution, i.e. 5 to 10 minutes. Along with that, a significant amount of daily precipitation totals from the late 19th and the 20th century, either elaborated from pluviograph strip charts or simply derived from bucket rain gauges, still exists in paper form, but it has never been digitalized. Within a project partly-funded by the Operational Programme of the European Union "Italia-Francia Marittimo", the Regional Environmental Protection Agency of Sardinia and the University of Cagliari will recover both the high-resolution rainfall signals and the older time series of daily totals recorded by a large number of pluviographs belonging to the historical monitoring networks of the island of Sardinia. Such data

  4. A Complete Holocene High-resolution Multiproxy Climate Record from the Northern Great Plains

    NASA Astrophysics Data System (ADS)

    Grimm, E. C.; Donovan, J. J.; Brown, K. J.

    2010-12-01

    The decadal-resolution multiproxy (mineralogy, pollen, charcoal) record from Kettle Lake, North Dakota encompasses the entire Holocene, with a chronology established by over 50 AMS radiocarbon dates. This record exhibits millennial scale trends evident in other lower-resolution studies, but with substantially greater detail on the rapidity and timing of major climatic shifts and on short-term climate variability. This record utilizes the rate of endogenic carbonate sedimentation, which depends on the rate of groundwater flow into the lake, as a sensitive proxy for precipitation, especially suitable for lakes supported by water from highly permeable carbonate-rich aquifers. Independent cluster analyses of mineral and pollen data reveal major Holocene mode shifts at 10.73 ka (ka = cal yr BP), 9.25 ka, and 4.44 ka. The early Holocene, 11.7-9.25 ka, is generally wet, with perhaps a trend to higher evaporation associated with warming temperatures. A switch from calcite to aragonite deposition is associated with a severe, but brief, drought at 10.73 ka. From 10.73 ka to 9.25 ka, a generally humid climate is punctuated at 100-300 yr intervals by brief droughts, including the most severe drought of the entire Holocene at 9.25 ka. The number of droughts during this period, including the bracketing droughts at 10.73 ka and 9.25 ka, is comparable to the number of Lake Agassiz recessions and outbursts during this period. Furthermore, the bracketing droughts correspond in age to the largest draw downs (30 and 58 m). Based on this evidence, we propose that droughts evident in the aragonite record at Kettle Lake were induced by either (a) local climate effects related to decreased size of or increased distance from Lake Agassiz, or (b) teleconnections with North Atlantic thermohaline changes associated with Agassiz outbursts. With the retreat of Lake Agassiz far to the north from the Stonewall beach at 9.25 ka, the “Agassiz lake effect” on NGP humidity was removed, and NGP

  5. High-resolution quantitative determination of dielectric function by using scattering scanning near-field optical microscopy.

    PubMed

    Tranca, D E; Stanciu, S G; Hristu, R; Stoichita, C; Tofail, S A M; Stanciu, G A

    2015-07-03

    A new method for high-resolution quantitative measurement of the dielectric function by using scattering scanning near-field optical microscopy (s-SNOM) is presented. The method is based on a calibration procedure that uses the s-SNOM oscillating dipole model of the probe-sample interaction and quantitative s-SNOM measurements. The nanoscale capabilities of the method have the potential to enable novel applications in various fields such as nano-electronics, nano-photonics, biology or medicine.

  6. A High-Resolution Carbonate Record from Holocene IMAGES core MD992286, Skagerrak

    NASA Astrophysics Data System (ADS)

    Gyllencreutz, R.

    2003-04-01

    Marine sediments in the 32.4 m long piston core MD99-2286, provides a continuous and detailed palaeoceanographic and palaeoenvironmenatal record of the last 12 000 years in the Skagerrak. The investigated area is characterised by high sedimentation rates and intense water mass mixing, as a branch of the North Atlantic Current turns anti-clockwise, slows down and becomes mixed with other waters to form the Norwegian Coastal Current. Present-day SSTs in Skagerrak are strongly linked to the NAO-index. Carbonate content in core MD99-2286 was measured using coulometry with 5-cm resolution. The carbonate record shows a steep rise from 9 to 15 % carbonate from 10 ka to 8 ka, and then a gradual decreasing trend down to about 12 % around 4 500 cal y BP. The timing of the peak at 8 ka coincides with the opening of the English Channel. The most likely processes controlling carbonate content in the Skagerrak sediments are redeposition of older carbonate particles (mainly controlled by the Jutland Current) and primary productivity competing with dilution by terrigenous material. The carbonate record from MD99-2286 correlates with a palaeotemperature reconstruction based on oxygen isotopes from a speleothem in northern Norway. The correlation is positive in the interval 9 ka to about 5 ka, and negative from 5 ka to present. The timing of the shift in correlation matches the establishment of the modern circulation pattern, which is marked by a hydrographic shift at 5.5 ka, interpreted from grain size and biostratigraphical data in a core from Skagen. This shift is manifested by an increase in the Jutland Current and stronger inflow of saline North Sea water to Skagerrak and Kattegat. The CALYPSO-corer used for core MD99-2286 is believed to disturb the topmost sediments in cores. Therefore, a 2.5 m long gravity core, Sk000209-2, was retrieved from a nearby location in order to get full recovery of the surface sediments. Age control in core MD99-2286 is obtained from 20 AMS C-14

  7. A high resolution record of chlorine-36 nuclear-weapons-tests fallout from Central Asia

    USGS Publications Warehouse

    Green, J.R.; Cecil, L.D.; Synal, H.-A.; Santos, J.; Kreutz, K.J.; Wake, C.P.

    2004-01-01

    The Inilchek Glacier, located in the Tien Shan Mountains, central Asia, is unique among mid-latitude glaciers because of its relatively large average annual accumulation. In July 2000, two ice cores of 162 and 167 meters (m) in length were collected from the Inilchek Glacier for (chlorine-36) 36Cl analysis a part of a collaborative international effort to study the environmental changes archived in mid-latitude glaciers worldwide. The average annual precipitation at the collection site was calculated to be 1.6 m. In contrast, the reported average annual accumulations at the high-latitude Dye-3 glacial site, Greenland, the mid-latitude Guliya Ice Cap, China, and the mid-latitude Upper Fremont Glacier, Wyoming, USA, were 0.52, 0.16 and 0.76 m, respectively. The resolution of the 36Cl record in one of the Inilchek ice cores was from 2 to 10 times higher than the resolution of the records at these other sites and could provide an opportunity for detailed study of environmental changes that have occurred over the past 150 years. Despite the differences in accumulation among these various glacial sites, the 36Cl profile and peak concentrations for the Inilchek ice core were remarkably similar in shape and magnitude to those for ice cores from these other sites. The 36Cl peak concentration from 1958, the year during the mid-1900s nuclear-weapons-tests period when 36Cl fallout was largest, was preserved in the Inilchek core at a depth of 90.56 m below the surface of the glacier (74.14-m-depth water equivalent) at a concentration of 7.7 ?? 105 atoms of 36Cl/gram (g) of ice. Peak 36Cl concentrations from Dye-3, Guliya and the Upper Fremont glacial sites were 7.1 ?? 105, 5.4 ?? 105 and 0.7 ?? 105 atoms of 36Cl/g of ice, respectively. Measurements of 36Cl preserved in ice cores improve estimates of historical worldwide atmospheric deposition of this isotope and allow the sources of 36Cl in ground water to be better identified. ?? 2004 Published by Elsevier B.V.

  8. A high resolution record of chlorine-36 nuclear-weapons-tests fallout from Central Asia

    NASA Astrophysics Data System (ADS)

    Green, J. R.; Cecil, L. D.; Synal, H.-A.; Santos, J.; Kreutz, K. J.; Wake, C. P.

    2004-08-01

    The Inilchek Glacier, located in the Tien Shan Mountains, central Asia, is unique among mid-latitude glaciers because of its relatively large average annual accumulation. In July 2000, two ice cores of 162 and 167 meters (m) in length were collected from the Inilchek Glacier for (chlorine-36) 36Cl analysis a part of a collaborative international effort to study the environmental changes archived in mid-latitude glaciers worldwide. The average annual precipitation at the collection site was calculated to be 1.6 m. In contrast, the reported average annual accumulations at the high-latitude Dye-3 glacial site, Greenland, the mid-latitude Guliya Ice Cap, China, and the mid-latitude Upper Fremont Glacier, Wyoming, USA, were 0.52, 0.16 and 0.76 m, respectively. The resolution of the 36Cl record in one of the Inilchek ice cores was from 2 to 10 times higher than the resolution of the records at these other sites and could provide an opportunity for detailed study of environmental changes that have occurred over the past 150 years. Despite the differences in accumulation among these various glacial sites, the 36Cl profile and peak concentrations for the Inilchek ice core were remarkably similar in shape and magnitude to those for ice cores from these other sites. The 36Cl peak concentration from 1958, the year during the mid-1900s nuclear-weapons-tests period when 36Cl fallout was largest, was preserved in the Inilchek core at a depth of 90.56 m below the surface of the glacier (74.14-m-depth water equivalent) at a concentration of 7.7 × 105 atoms of 36Cl/gram (g) of ice. Peak 36Cl concentrations from Dye-3, Guliya and the Upper Fremont glacial sites were 7.1 × 105, 5.4 × 105 and 0.7 × 105 atoms of 36Cl/g of ice, respectively. Measurements of 36Cl preserved in ice cores improve estimates of historical worldwide atmospheric deposition of this isotope and allow the sources of 36Cl in ground water to be better identified.

  9. High-Resolution Pollen Record of Deglacial Climate Variability in Central Florida

    NASA Astrophysics Data System (ADS)

    Willard, D. A.; Bernhardt, C. E.; Edgar, T.

    2004-12-01

    Pollen evidence from lacustrine sediments in Tampa Bay, Florida document considerable climatic variability superimposed on deglacial warming in the subtropics. Nine radiocarbon dates on well-preserved mollusk shells provide a reliable chronology of continuous sedimentation from 20 ka to 11.5 ka; examination of pollen assemblages from 2 cm increments within the lacustrine unit provide temporal resolution averaging one sample every 45 years. During the glacial maximum, much drier and cooler than modern conditions are indicated by pollen assemblages enriched in Chenopodiaceae, Carya, Poaceae and Ambrosia. Increased abundance of Pinus pollen between 17.4 ka and 15 ka indicates the existence of warmer, wetter conditions in the interval including Heinrich Event 1. After a reversion to drier, cooler conditions at about 15 ka, Pinus pollen abundance increased again at 14 ka. Combined with the near loss of Carya pollen, these data suggest attainment of nearly modern climatic conditions during the Bolling-Allerod (14-13 ka). However, variability within the Bolling-Allerod is documented by shifts to deglacial-equivalent vegetation during the Older Dryas (13.4-13.6 ka). The Younger Dryas (12.9-11.6 ka) is characterized by two distinct phases: from 12.9-11.9 ka, the increased abundance of Chenopodiaceae and Quercus mark drier, possibly cooler conditions comparable to those of the deglacial (17.4 - 14 ka). From 11.9-11.5 ka, strong dominance of herbaceous Chenopodiaceae is similar to full-glacial conditions, indicating much drier, possibly cooler conditions. Comparison of these paleoclimatic patterns with marine records from Orca Basin in the Gulf of Mexico indicate close temporal correspondence in warming of atmospheric and sea-surface temperatures. Pollen evidence also suggests that significant warming began around 17.4 ka, before the onset of Heinrich Event 1, as has been documented at other sites in the North Atlantic Ocean and Europe. This record of deglacial atmospheric

  10. High-resolution retinal imaging through open-loop adaptive optics.

    PubMed

    Li, Chao; Xia, Mingliang; Li, Dayu; Mu, Quanquan; Xuan, Li

    2010-01-01

    Using the liquid crystal spatial light modulator (LC-SLM) as the wavefront corrector, an open-loop adaptive optics (AO) system for fundus imaging in vivo is constructed. Compared with the LC-SLM closed-loop AO system, the light energy efficiency is increased by a factor of 2, which is helpful for the safety of fundus illumination in vivo. In our experiment, the subjective accommodation method is used to precorrect the defocus aberration, and three subjects with different myopia 0, -3, and -5 D are tested. Although the residual wavefront error after correction cannot to detected, the fundus images adequately demonstrate that the imaging system reaches the resolution of a single photoreceptor cell through the open-loop correction. Without dilating and cyclopleging the eye, the continuous imaging for 8 s is recorded for one of the subjects.

  11. High-resolution retinal imaging through open-loop adaptive optics

    NASA Astrophysics Data System (ADS)

    Li, Chao; Xia, Mingliang; Li, Dayu; Mu, Quanquan; Xuan, Li

    2010-07-01

    Using the liquid crystal spatial light modulator (LC-SLM) as the wavefront corrector, an open-loop adaptive optics (AO) system for fundus imaging in vivo is constructed. Compared with the LC-SLM closed-loop AO system, the light energy efficiency is increased by a factor of 2, which is helpful for the safety of fundus illumination in vivo. In our experiment, the subjective accommodation method is used to precorrect the defocus aberration, and three subjects with different myopia 0, -3, and -5 D are tested. Although the residual wavefront error after correction cannot to detected, the fundus images adequately demonstrate that the imaging system reaches the resolution of a single photoreceptor cell through the open-loop correction. Without dilating and cyclopleging the eye, the continuous imaging for 8 s is recorded for one of the subjects.

  12. Characterization of rock thermal conductivity by high-resolution optical scanning

    USGS Publications Warehouse

    Popov, Y.A.; Pribnow, D.F.C.; Sass, J.H.; Williams, C.F.; Burkhardt, H.

    1999-01-01

    We compared thress laboratory methods for thermal conductivity measurements: divided-bar, line-source and optical scanning. These methods are widely used in geothermal and petrophysical studies, particularly as applied to research on cores from deep scientific boreholes. The relatively new optical scanning method has recently been perfected and applied to geophysical problems. A comparison among these methods for determining the thermal conductivity tensor for anisotropic rocks is based on a representative collection of 80 crystalline rock samples from the KTB continental deep borehole (Germany). Despite substantial thermal inhomogeneity of rock thermal conductivity (up to 40-50% variation) and high anisotropy (with ratios of principal values attaining 2 and more), the results of measurements agree very well among the different methods. The discrepancy for measurements along the foliation is negligible (<1%). The component of thermal conductivity normal to the foliation reveals somewhat larger differences (3-4%). Optical scanning allowed us to characterize the thermal inhomogeneity of rocks and to identify a three-dimensional anisotropy in thermal conductivity of some gneiss samples. The merits of optical scanning include minor random errors (1.6%), the ability to record the variation of thermal conductivity along the sample, the ability to sample deeply using a slow scanning rate, freedom from constraints for sample size and shape, and quality of mechanical treatment of the sample surface, a contactless mode of measurement, high speed of operation, and the ability to measure on a cylindrical sample surface. More traditional methods remain superior for characterizing bulk conductivity at elevated temperature.Three laboratory methods including divided-bar, line-source and optical scanning are widely applied in geothermal and petrophysical studies. In this study, these three methods were compared for determining the thermal conductivity tensor for anisotropic rocks

  13. The 8.2 kyr event recorded at high resolution by a speleothem from the Northern French Alps

    NASA Astrophysics Data System (ADS)

    Couchoud, Isabelle; Drysdale, Russell; Hellstrom, John; Perrette, Yves

    2016-04-01

    Continental records of Holocene climate variability in the Alps are scarce and they rarely document the "8.2 kyr event". Several reasons have been proposed: records do not have sufficient resolution, are not precisely dated or the proxies are not sufficiently sensitive to climate variations associated with this event. The European records of adequate resolution generally allow the identification of an anomaly but its characterisation is unclear: it seems that the event has triggered significant impacts only for a few decades and mainly expressed in winter (whereas most archives are more sensitive to summer conditions). Thus, the detailed characterisation of the impacts of this event requires records with sub-annual resolution and preferably sensitive to winter conditions. Here we present a multi-proxy, high-resolution analysis of this event, as recorded by a stalagmite of the Bauges massif (Northern French Alps), at ~1400 m altitude. Comparison of these results with other regional and distant data allows for a discussion about climatic impacts and teleconnections at this time.

  14. Late Permian-earliest Triassic high-resolution organic carbon isotope and palynofacies records from Kap Stosch (East Greenland)

    NASA Astrophysics Data System (ADS)

    Sanson-Barrera, Anna; Hochuli, Peter A.; Bucher, Hugo; Schneebeli-Hermann, Elke; Weissert, Helmut; Adatte, Thierry; Bernasconi, Stefano M.

    2015-10-01

    During and after the end Permian mass extinction terrestrial and marine biota underwent major changes and reorganizations. The latest Permian and earliest Triassic is also characterized by major negative carbon isotope shifts reflecting fundamental changes in the carbon cycle. The present study documents a high-resolution bulk organic carbon isotope record and palynofacies analysis spanning the latest Permian-earliest Triassic of East Greenland. An almost 700 meter thick composite section from Kap Stosch allowed discriminating 6 chemostratigraphic intervals that provide the basis for the correlation with other coeval records across the world, and for the recognition of basin wide transgressive-regressive events documenting tectonic activity during the opening of the Greenland-Norway Basin. The identification of the main factors that influenced the organic carbon isotope signal during the earliest Triassic (Griesbachian to Dienerian) was possible due to the combination of bulk organic carbon isotope, palynofacies and Rock-Eval data. Two negative carbon isotopic shifts in the Kap Stosch record can be correlated with negative shifts recorded in coeval sections across the globe. A first negative shift precedes the base of the Triassic as defined by the first occurrence of the conodont Hindeodus parvus in the Meishan reference section, and the second one coincides with the suggested Griesbachian-Dienerian boundary. This new organic carbon isotope record from the extended Kap Stosch section from the Boreal Realm documents regional and global carbon cycle signals of the interval between the latest Palaeozoic and the onset of the Mesozoic.

  15. Plio-Pleistocene climate sensitivity evaluated using high-resolution CO2 records.

    PubMed

    Martínez-Botí, M A; Foster, G L; Chalk, T B; Rohling, E J; Sexton, P F; Lunt, D J; Pancost, R D; Badger, M P S; Schmidt, D N

    2015-02-05

    Theory and climate modelling suggest that the sensitivity of Earth's climate to changes in radiative forcing could depend on the background climate. However, palaeoclimate data have thus far been insufficient to provide a conclusive test of this prediction. Here we present atmospheric carbon dioxide (CO2) reconstructions based on multi-site boron-isotope records from the late Pliocene epoch (3.3 to 2.3 million years ago). We find that Earth's climate sensitivity to CO2-based radiative forcing (Earth system sensitivity) was half as strong during the warm Pliocene as during the cold late Pleistocene epoch (0.8 to 0.01 million years ago). We attribute this difference to the radiative impacts of continental ice-volume changes (the ice-albedo feedback) during the late Pleistocene, because equilibrium climate sensitivity is identical for the two intervals when we account for such impacts using sea-level reconstructions. We conclude that, on a global scale, no unexpected climate feedbacks operated during the warm Pliocene, and that predictions of equilibrium climate sensitivity (excluding long-term ice-albedo feedbacks) for our Pliocene-like future (with CO2 levels up to maximum Pliocene levels of 450 parts per million) are well described by the currently accepted range of an increase of 1.5 K to 4.5 K per doubling of CO2.

  16. A high-resolution angiosperm pollen reference record covering Albian mid-latitude coastal deposits (Lusitanian Basin, Portugal)

    NASA Astrophysics Data System (ADS)

    Horikx, Maurits; Dinis, Jorge L.; Heimhofer, Ulrich

    2013-04-01

    The Lusitanian Basin in Portugal is one of the most important areas to investigate the rise and radiation of early angiosperms. Here, important micro-, macro- and mesofossil remains including pollen, reproductive organs, fruits and seeds have been found. In this study, a high-resolution Early to Late Albian pollen record from a thick (~160m) coastal succession in the Lusitanian Basin containing mixed carbonate-siliciclastic near-shore deposits is generated. The outcrop is located near the town of Ericeira (São Julião) and exhibits some important new features compared to existing records from the Lusitanian basin. The comparatively proximal depositional setting and high sedimentation rate of the São Julião outcrop is well suited for high-resolution palynological sampling compared to previously studied, more distal outcrops. In addition, the succession covers almost the entire Albian including a thick interval representing Late Albian strata. Dating of the succession was obtained using dinoflagellate cyst biostratigraphy, bulk C-isotope analysis and strontium isotope analysis of low-Mg oysters and rudist shells. The high-resolution pollen record shows a distinct radiation pattern of early angiosperm pollen as well as significant changes in the accompanying palynoflora. During most of the section gymnosperm pollen types such as Classopollis spp., Inaperturopollenites spp. and Exesipollenites spp. are dominant. Angiosperm pollen abundances do not exceed 20%, although angiosperms increase slightly from the Early Albian onwards. Monoaperturate grains of magnoliid or monocot affinity remain the most dominant angiosperm pollen type, both in abundances and diversity. Tricolpate and zonoaperturate pollen grains are also present. In addition, the occurrence of several odd-shaped Dichastopollenites-type pollen types is intriguing. The palynological results indicate a warm and dry climate during most of the Albian, although a rise in the spores over pollen ratio in the

  17. A high-resolution record of carbon accumulation rates during boreal peatland initiation

    NASA Astrophysics Data System (ADS)

    Pendea, I. F.; Chmura, G. L.

    2012-07-01

    Boreal peatlands are a major global C sink, thus having important feedbacks to climate. A decreased concentration in atmospheric CO2 7000-10 000 yr ago has been linked to variations in peatland C accumulation rates attributed to a warm climate and increased productivity. Yet, this period also corresponds to early stages of peatland development (as peatland was expanding) following retreat of ice sheets and increases in C storage could be associated with wetland evolution via lake filling or following marine shoreline emergence. Unravelling past links amongst peatland dynamics, C storage, and climate will help us assess potential feedbacks from future changes in these systems, but most studies are hampered by low temporal resolution. Here we provide a decadal scale C accumulation record for a fen that has begun transformation from salt marsh within the last 70 yr on the isostatically rebounding coast of James Bay, Québec. We determined time frames for wetland stages using palynological analyses to reconstruct ecological change and 210Pb and 137Cs to date the deposit. The average short-term C accumulation rates during the low and high tidal marsh and incipient fen stage (42, 87 and 182 g C m-2 yr-1, respectively) were as much as six times higher than the global long-term (millennial) average for northern peatlands. We suggest that the atmospheric CO2 flux during the early Holocene could be attributed, in part, to wetland evolution associated with isostatic rebound, which makes land for new wetland formation. Future climate warming will increase eustatic sea level, decrease rates of land emergence and formation of new coastal wetlands, ultimately decreasing rates of C storage of wetlands on rebounding coastlines.

  18. High resolution record of carbon accumulation rates during boreal peatland initiation

    NASA Astrophysics Data System (ADS)

    Florin Pendea, I.; Chmura, G. L.

    2012-01-01

    Boreal peatlands are a major global C sink, thus having important feedbacks to climate. A decreased concentration in atmospheric CO2 7000-10 000 years ago has been linked to variations in peatland C accumulation rates attributed to warm climate change and increased productivity. Yet, this period also corresponds to early stages of peatland development (as peatland was expanding) following retreat of ice sheets and increases in C storage could be associated with wetland evolution via lake filling or following marine shoreline emergence. Unravelling past links amongst peatland dynamics, C storage, and climate will help us assess potential feedbacks from future changes in these systems, but most studies are hampered by low temporal resolution. Here we provide a decadal scale C accumulation record for a fen that has transformed from salt marsh within the last 70 yr on the isostatically rebounding coast of James Bay, Québec. We determined time frames for wetland stages using palynological analyses to reconstruct ecological change and 210Pb and 137Cs to date the deposit. The C accumulation rates during the tidal marsh and fen stage (87 and 182 g C m-2 yr-1, respectively), were as much as six times higher than the global average for northern peatlands. We suggest that the atmospheric CO2 flux during the early Holocene could be attributed, in part, to wetland evolution associated with isostatic rebound which makes land for new wetland formation. Future climate warming will increase eustatic sea level, decrease rates of land emergence and formation of new coastal wetlands, ultimately decreasing rates of C storage of wetlands on rebounding coastlines.

  19. High-resolution Fiber-optic Microendoscopy for in situ Cellular Imaging

    PubMed Central

    Pierce, Mark; Yu, Dihua; Richards-Kortum, Rebecca

    2011-01-01

    Many biological and clinical studies require the longitudinal study and analysis of morphology and function with cellular level resolution. Traditionally, multiple experiments are run in parallel, with individual samples removed from the study at sequential time points for evaluation by light microscopy. Several intravital techniques have been developed, with confocal, multiphoton, and second harmonic microscopy all demonstrating their ability to be used for imaging in situ 1. With these systems, however, the required infrastructure is complex and expensive, involving scanning laser systems and complex light sources. Here we present a protocol for the design and assembly of a high-resolution microendoscope which can be built in a day using off-the-shelf components for under US$5,000. The platform offers flexibility in terms of image resolution, field-of-view, and operating wavelength, and we describe how these parameters can be easily modified to meet the specific needs of the end user. We and others have explored the use of the high-resolution microendoscope (HRME) in in vitro cell culture 2-5, in excised 6 and living animal tissues 2,5, and in human tissues in vivo 2,7. Users have reported the use of several different fluorescent contrast agents, including proflavine 2-4, benzoporphyrin-derivative monoacid ring A (BPD-MA) 5, and fluoroscein 6,7, all of which have received full, or investigational approval from the FDA for use in human subjects. High-resolution microendoscopy, in the form described here, may appeal to a wide range of researchers working in the basic and clinical sciences. The technique offers an effective and economical approach which complements traditional benchtop microscopy, by enabling the user to perform high-resolution, longitudinal imaging in situ. PMID:21248707

  20. High-resolution fiber-optic microendoscopy for in situ cellular imaging.

    PubMed

    Pierce, Mark; Yu, Dihua; Richards-Kortum, Rebecca

    2011-01-11

    Many biological and clinical studies require the longitudinal study and analysis of morphology and function with cellular level resolution. Traditionally, multiple experiments are run in parallel, with individual samples removed from the study at sequential time points for evaluation by light microscopy. Several intravital techniques have been developed, with confocal, multiphoton, and second harmonic microscopy all demonstrating their ability to be used for imaging in situ. With these systems, however, the required infrastructure is complex and expensive, involving scanning laser systems and complex light sources. Here we present a protocol for the design and assembly of a high-resolution microendoscope which can be built in a day using off-the-shelf components for under US$5,000. The platform offers flexibility in terms of image resolution, field-of-view, and operating wavelength, and we describe how these parameters can be easily modified to meet the specific needs of the end user. We and others have explored the use of the high-resolution microendoscope (HRME) in in vitro cell culture, in excised and living animal tissues, and in human tissues in vivo. Users have reported the use of several different fluorescent contrast agents, including proflavine, benzoporphyrin-derivative monoacid ring A (BPD-MA), and fluoroscein, all of which have received full, or investigational approval from the FDA for use in human subjects. High-resolution microendoscopy, in the form described here, may appeal to a wide range of researchers working in the basic and clinical sciences. The technique offers an effective and economical approach which complements traditional benchtop microscopy, by enabling the user to perform high-resolution, longitudinal imaging in situ.

  1. A New Method of Obtaining High-Resolution Paleoclimate Records from Speleothem Fluid Inclusions

    NASA Astrophysics Data System (ADS)

    Logan, A. J.; Horton, T. W.

    2010-12-01

    a minimum quantity of ~100nL water was required. In contrast to liquid water analyses, carbonate inclusion waters gave highly variable results. As plenty of signal was produced from relatively small sample sizes (~20 mg), the observed isotopic variation most likely reflects fractionation during fluid extraction, or natural isotopic variability. Additional tests and modifications to the extraction procedure are in progress, using a recently collected New Zealand stalagmite from a West Coast cave (DOC collection permit WC-27462-GEO). U-Th age data will accompany a paleoclimate record from this stalagmite obtained using standard carbonate analytical techniques, and compared to the results from our new fluid inclusion analyses.

  2. A High-Resolution Record of Warm Water Inflow and Iceberg Calving in Upernavik Isfjord During the Past 150 Years.

    NASA Astrophysics Data System (ADS)

    Vermassen, F.; Andresen, C. S.; Sabine, S.; Holtvoeth, J.; Cordua, A. E.; Wangner, D. J.; Dyke, L. M.; Kjaer, K. H.; Kokfelt, U.; Haubner, K.

    2016-12-01

    There is a growing body of evidence demonstrating that changes in warm water inflow to Greenlandic fjords are linked to the rapid retreat of marine-terminating outlet glaciers. This process is thought to be responsible for a substantial component of the increased mass loss from the Greenland Ice Sheet over the last two decades. Sediment cores from glaciated fjords provide high-resolution sedimentological and biological proxy records which can be used to evaluate the interplay of warm water inflow and glacier calving over recent time scales. In this study, multiple short cores ( 2 m) from Upernavik Isfjord, West Greenland, were analysed to establish a multi-proxy record of glacier behaviour and oceanographic conditions that spans the past 150 years. The down-core variation in the amount of ice-rafted debris reveals periods of increased glacier calving, and biomarker proxies are used to reconstruct variability in the inflow of warm, Atlantic-sourced water to the fjord. Measurements of the sortable silt grain size are used to reconstruct bottom-current strength; periods of vigorous current flow are assumed to be due to enhanced warm water inflow. Finally, a record of glacier terminus position changes, derived from historical observations and satellite imagery, allows comparison of our new proxy records with the retreat of the ice margin from 1849 onwards. We use these data to assess the relative importance of mechanisms controlling the (rapid) retreat of marine-terminating glaciers in Upernavik Isfjord.

  3. A high-resolution record of early Miocene Antarctic glacial history from ODP Site 1165, Prydz Bay

    NASA Astrophysics Data System (ADS)

    Williams, Trevor; Handwerger, David

    2005-06-01

    ODP Site 1165, located 400 km northwest of Prydz Bay, contains a high-resolution early Miocene record of pulses of ice-rafted debris (IRD) originating from the ancestral Lambert Glacier system and the Antarctic coast to the east. The 520 m of early Miocene sediments consist of dark gray claystone with silt laminae (contourites) alternating with decimeter-scale layers of greenish-gray bioturbated claystone that commonly contain ice-rafted debris. Downhole logs also record the alternations between the two facies: the IRD-bearing greenish-grey claystone corresponds to high resistivity and density values because of increased cementation by silica, and corresponds to lower natural gamma radiation values because of diluted clay content. The downhole logs thus allow a continuous and detailed stratigraphic record of the IRD-bearing layers to be obtained. The IRD-bearing layers represent deglaciations and interglacials, when a high flux of icebergs with incorporated material were melting out over the site; the contourite-rich layers represent glacials, when the Polar Current over the site was relatively strong. The sequence is dated by magnetostratigraphy, and the timing of the major IRD pulses is paced by orbital eccentricity, indicating that the volume of the East Antarctic ice sheet also fluctuates on this timescale. After 19.7 Ma, minor precessional and subprecessional IRD layers appear in the record, indicating that the ice sheet becomes more prone to deglaciation through this interval, perhaps associated with the gradual warming trend through the early Miocene.

  4. What can we learn about the dynamics of DO-events from studying the high resolution ice core records?

    NASA Astrophysics Data System (ADS)

    Ditlevsen, Peter

    2017-04-01

    The causes for and possible predictions of rapid climate changes are poorly understood. The most pronounced changes observed, beside the glacial terminations, are the Dansgaard-Oeschger events. Present day general circulation climate models simulating glacial conditions are not capable of reproducing these rapid shifts. It is thus not known if they are due to bifurcations in the structural stability of the climate or if they are induced by stochastic fluctuations. By analyzing a high resolution ice core record we exclude the bifurcation scenario, which strongly suggests that they are noise induced and thus have very limited predictability. Ref: Peter Ditlevsen, "Tipping points in the climate system", in Nonlinear and Stochastic Climate Dynamics, Cambridge University Press (C. Franzke and T. O'Kane, eds.) (2016) P. D. Ditlevsen and S. Johnsen, "Tipping points: Early warning and wishful thinking", Geophys. Res. Lett., 37, L19703, 2010

  5. Hypoxia in high-resolution sediment records: reconstructing the California Current Oxygen Minimum Zone on multi-decadal timescales

    NASA Astrophysics Data System (ADS)

    Moffitt, S. E.; Hill, T. M.

    2012-12-01

    The recent deglaciation event is an ideal laboratory to study the rapid expansion of Oxygen Minimum Zones (OMZs) and the ecological ramifications of such events. Santa Barbara Basin (SBB) sediments are high-resolution archives of seafloor ecosystems, recording both global-scale climate and regional-scale hydrographic events. Seafloor hypoxia in the California Current Ecosystem (CCE) is caused by OMZs in intermediate water depths (300-1200 m), and produces striking evidence in SBB sediment archives. We construct a vertical transect of proxies across SBB (34° 15'N, 119° 45'W) using a core from 418 m water depth (MV0811-15JC), and previously investigated cores from 440 m (MD02-2504) and 570 m (MD02-2503) water depths. Benthic foraminiferal assemblages and planktonic δ18O proxies were quantified at all three depths, while benthic invertebrate communities were quantified in the shallowest core at a 1-cm resolution (~10 years). This high-resolution invertebrate record provides a window into rapid, decadal-scale environmental change in continental margin ecosystems. Seafloor biodiversity is highly variable on 10-10^4 year timescales (across Protist, Mollusc, Arthropod and Echinoderm taxonomic groups), and is tightly coupled to both regional-scale environmental change and global-scale climate events. Additionally, we provide evidence that strongly hypoxic waters shoaled to <300 m water depth at Termination 1A (14.7 ka), implying that the upper boundary of the regional OMZ can expand >150 m on multi-decadal timescales. These data confirm that OMZs have rapidly expanded in the CCE during previous events of global-scale warming, and that continental margin seafloor biodiversity is variable on previously undescribed timescales.

  6. Optical coherence microscopy in 1700-nm spectral band for high-resolution deep-tissue imaging (Conference Presentation)

    NASA Astrophysics Data System (ADS)

    Yamanaka, Masahito; Teranishi, Tatsuhiro; Kawagoe, Hiroyuki; Nishizawa, Norihiko

    2017-02-01

    Optical coherence microscopy (OCM) is a high-resolution imaging technique based on optical coherence tomography and confocal microscopy. The recent studies on OCM operating at 800-1300 nm spectral region have shown that OCM enables to visualize micrometer- or sub-micrometer-scale structures of animal tissues. Although OCMs offers such high-resolution label-free imaging capability of animal tissues, the imaging depth was restricted by multiple light scattering and light absorption of water in samples. Here, for high-resolution deep-tissue imaging, we developed an OCM in the 1700-nm spectral band by using a supercontinuum (SC) source with a Gaussian-like spectral shape in the wavelength region. Recently, it has been reported that the 1700-nm spectral band is a promising choice for enhancing the imaging depth in the observation of turbid scattering tissues because of the low attenuation coefficient of light. In this study, to clarify that the 1700-nm OCM has a potential to realize the enhanced imaging depth, we compared the attenuation of the signal-to-noise ratio between the 1700-nm and 1300-nm OCM imaging of a mouse brain under the same signal detection sensitivity condition. The result shows that the 1700-nm OCM enables us to achieve the enhanced imaging depth. In this 1700-nm OCM, we also confirmed that the lateral resolution of 1.3 µm and axial resolution of 2.8 µm in tissue were achieved.

  7. Cellular basis for trigger and maintenance of ventricular fibrillation in the Brugada syndrome model: high-resolution optical mapping study.

    PubMed

    Aiba, Takeshi; Shimizu, Wataru; Hidaka, Ichiro; Uemura, Kazunori; Noda, Takashi; Zheng, Can; Kamiya, Atsunori; Inagaki, Masashi; Sugimachi, Masaru; Sunagawa, Kenji

    2006-05-16

    We examined how repolarization and depolarization abnormalities contribute to the development of extrasystoles and subsequent ventricular fibrillation (VF) in a model of the Brugada syndrome. Repolarization and depolarization abnormalities have been considered to be mechanisms of the coved-type ST-segment elevation (Brugada-electrocardiogram [ECG]) and development of VF in the Brugada syndrome. We used high-resolution (256 x 256) optical mapping techniques to study arterially perfused canine right ventricular wedges (n = 20) in baseline and in the Brugada-ECG produced by administration of terfenadine (5 micromol/l), pinacidil (2 micromol/l), and pilsicainide (5 micromol/l). We recorded spontaneous episodes of phase 2 re-entrant (P2R)-extrasystoles and subsequent self-terminating polymorphic ventricular tachycardia (PVT) or VF under the Brugada-ECG condition and analyzed the epicardial conduction velocity and action potential duration (APD) restitutions in each condition. Forty-one episodes of spontaneous P2R-extrasystoles in the Brugada-ECG were successfully mapped in 9 of 10 preparations, and 33 of them were originated from the maximum gradient of repolarization (GR(max): 176 +/- 54 ms/mm) area in the epicardium, leading to PVT (n = 12) or VF (n = 5). The epicardial GR(max) was not different between PVT and VF. Wave-break during the first P2R-extrasystole produced multiple wavelets in all VF cases, whereas no wave-break or wave-break followed by wave collision and termination occurred in PVT cases. Moreover, conduction velocity restitution was shifted lower and APD restitution was more variable in VF cases than in PVT cases. Steep repolarization gradient in the epicardium but not endocardium develops P2R-extrasystoles in the Brugada-ECG condition, which might degenerate into VF by further depolarization and repolarization abnormalities.

  8. Optical sedimentation recorder

    DOEpatents

    Bishop, James K.B.

    2014-05-06

    A robotic optical sedimentation recorder is described for the recordation of carbon flux in the oceans wherein both POC and PIC particles are captured at the open end of a submersible sampling platform, the captured particles allowed to drift down onto a collection plate where they can be imaged over time. The particles are imaged using three separate light sources, activated in sequence, one source being a back light, a second source being a side light to provide dark field illumination, and a third source comprising a cross polarized light source to illuminate birefringent particles. The recorder in one embodiment is attached to a buoyancy unit which is capable upon command for bringing the sedimentation recorder to a programmed depth below the ocean surface during recordation mode, and on command returning the unit to the ocean surface for transmission of recorded data and receipt of new instructions. The combined unit is provided with its own power source and is designed to operate autonomously in the ocean for extended periods of time.

  9. Mapping of Impervious Surfaces and Green Vegetation Fraction in Urban Areas Using High-Resolution Optical- and SAR- Data

    NASA Astrophysics Data System (ADS)

    Mack, Benjamin; Becker, Frauke; Menz, Gunther

    2010-12-01

    Impervious surfaces (IS) and vegetated surfaces (VS) are two important variables for urban planning. Both are related to surface biophysical processes (e. g. urban heat island) and the quality of life in urban environments. The new generation of spatially very high resolution satellite sensors (e.g. RapidEye, or TerraSAR-X) show promising capabilities to extract these variables in relevant detail and hence to overcome some of the limitations using traditional approaches. In this study, we show a simple workflow for the synergistic use of high-resolution optical and SAR data for the retrieval of detailed information of IS and VS. Here, the focus is on the processing step of vegetation extraction. Three approaches - linear Spectral Mixture Analysis, per-pixel, and segment-oriented classification - are compared. It will also be analyzed if an explicit shadow analysis leads to a significant improvement of the products.

  10. Three-dimensional imaging of eye surface pathologies and contact lens fit with high resolution spectral optical coherence tomography

    NASA Astrophysics Data System (ADS)

    Wojtkowski, M.; Kałużny, B.; Szkulmowska, A.; Bajraszewski, T.; Szkulmowski, M.; Targowski, P.; Kowalczyk, A.

    2007-02-01

    Purpose: To show potential of Spectral Optical Coherence Tomography system for high resolution, cross-sectional and three-dimensional imaging of eye surface pathologies. Methods: High-speed spectral OCT prototype instrument with 4.5 μm axial resolution was designed and constructed for clinical use. Measurements of anterior segment of human eye have been performed in ophthalmology clinic on 86 patients suffering various eye surface disorders including corneal dystrophies, corneal scars, conjunctival folds, keratoconus, bullus keratopathy, filtration blebs and other post-operative changes. Additionally, examinations of contact lens fit on 97 healthy corneas have been performed up to date. Results: High quality, high resolution cross-sectional images and three-dimensional reconstructions of cornea, conjunctiva and sclera of pathologic eyes together with examples of numerical analysis including segmentation of fluid in filtration blebs, scars and deposits are shown. Quantitative analysis of contact lens fit is demonstrated.

  11. Note: Autocollimation with ultra-high resolution and stability using telephoto objective together with optical enlargement and beam drift compensation

    SciTech Connect

    Zhu, Fan; Tan, Xinran; Tan, Jiubin; Fan, Zhigang

    2016-08-15

    An autocollimation (AC) setup with ultra-high resolution and stability for micro-angle measurement is presented. The telephoto objective, which is characterized in long focal length at a compact structure size, and the optical enlargement unit, which can magnify the image displacement to improve its measurement resolution and accuracy, are used to obtain an ultra-high measurement resolution of the AC. The common-path beam drift compensation is used to suppress the drift of measurement results, which is evident in the high-resolution AC, thus to obtain a high measurement stability. Experimental results indicate that an effective resolution of better than 0.0005 arc sec (2.42 nrad) over a measurement range of ±30 arc sec and a 2-h stability of 0.0061 arc sec (29.57 nrad) can be achieved.

  12. Ultrasensitive high resolution laser spectroscopy and its application to optical frequency standards

    NASA Astrophysics Data System (ADS)

    Ye, Jun

    1997-09-01

    Advanced laser stabilization techniques now enable one to lock laser frequencies onto line centers of natural atomic/molecular resonances with unprecedented precision and accuracy. In this dissertation we discuss our effort in utilizing these techniques to establish visible optical frequency standards. By summarizing our earlier results on frequency measurements of the 87Rb D2 line at 780 nm 127I2 hyperfine transitions at 532 nm, we show the advantage of using a higher quality reference line, usually characterized by its narrower linewidth, higher attainable signal-to-noise ratio and lower sensitivity toward external perturbations. We then present a novel approach of cavity-enhanced frequency modulation spectroscopy for ultra-sensitive detections. The powerful utility of this new technique in the field of frequency standards is demonstrated by probing saturated molecular overtone transitions in the visible and near infrared. Weakly-absorbing gases such as C2H2 and C2HD are placed inside an external high-finesse resonator to enhance their detection sensitivities. A frequency modulation technique is employed to achieve a shot noise limited signal-to- noise ratio. The rf modulation frequency is chosen to match the cavity's free spectral range in order to avoid the cavity-induced conversion of laser frequency noise into amplitude noise. The molecular saturated dispersion signal is directly recovered after demodulation of the cavity transmitted light. A record high integrated absorption sensitivity of 5× 10-13/ (1× 10-14/cm) (at 1 second averaging time) has been obtained. Systematic studies on this new technique are presented on topics of detection sensitivity, signal line shape, signal size and slope, and pressure dependent linewidth broadening and linecenter shift. A Nd:YAG laser is stabilized on the P(5) transition in the (ν2+3/ ν3) overtone band of C2HD at 1.064 μm. Its absolute frequency is established. The excellent signal- to-noise ratio produces a frequency

  13. Fabrication of high-resolution reflective scale grating for an optical encoder using a patterned self-assembly process

    NASA Astrophysics Data System (ADS)

    Fan, Shanjin; Jiang, Weitao; Li, Xuan; Yu, Haoyu; Lei, Biao; Shi, Yongsheng; Yin, Lei; Chen, Bangdao; Liu, Hongzhong

    2016-07-01

    Steel tape scale grating of a reflective incremental linear encoder has a key impact on the measurement accuracy of the optical encoder. However, it is difficult for conventional manufacturing processes to fabricate scale grating with high-resolution grating strips, due to process and material problems. In this paper, self-assembly technology was employed to fabricate high-resolution steel tape scale grating for a reflective incremental linear encoder. Graphene oxide nanoparticles were adopted to form anti-reflective grating strips of steel tape scale grating. They were deposited in the tape, which had a hydrophobic and hydrophilic grating pattern when the dispersion of the nanoparticles evaporated. A standard lift-off process was employed to fabricate the hydrophobic grating strips on the steel tape. Simultaneously, the steel tape itself presents a hydrophilic property. The hydrophobic and hydrophilic grating pattern was thus obtained. In this study, octafluorocyclobutane was used to prepare the hydrophobic grating strips, due to its hydrophobic property. High-resolution graphene oxide steel tape scale grating with a pitch of 20 μm was obtained through the self-assembly process. The photoelectric signals of the optical encoder containing the graphene oxide scale grating and conventional scale grating were tested under the same conditions. Comparison test results showed that the graphene oxide scale grating has a better performance in its amplitude and harmonic components than that of the conventional steel tape scale. A comparison experiment of position errors was also conducted, demonstrating an improvement in the positioning error of the graphene oxide scale grating. The comparison results demonstrated the applicability of the proposed self-assembly process to fabricate high-resolution graphene oxide scale grating for a reflective incremental linear encoder.

  14. High-Resolution Dust record of last glacial period (MIS 4 to MIS 2) from Talos Dome Ice Core

    NASA Astrophysics Data System (ADS)

    Mazzola, Claudia; Maggi, Valter; Delmonte, Barbara; Marino, Federica; Albani, Samuel

    2010-05-01

    Mineral dust trapped in Antarctic ice cores plays an important role in the study of past climate and atmospheric circulation variability in the Southern Hemisphere. In this work we investigate the Talos Dome (Northern Victoria Land, East Antarctica) ice core dust concentration record through a Laser Sensor (LS) technique. Analyses were performed in continuous as a part of the Continuous Flow Analysis (CFA) system, and discontinuously with Beckman Coulter©Counter for comparison and LS calibration. The LS device provided two basic outputs: (1) "bag" (1 m long sections) mean values and (2) high resolution (1 cm resolution) data. Both signals were processed in the contest of this work and two dust records, respectively at low and high resolution, were produced. Here we report the bag mean dust record from the end of the last deglaciation (about 12,000 years B.P.) to Marine Isotopic Stage 4 (MIS 4, 70,000 years BP). The comparison of the TALDICE LS and EPICA-Dome C dust record provides interesting information about climatic conditions of South Pacific-Ross Sea sector of Antarctica, and atmospheric circulation patterns during last glacial period, with a good agreement between MIS 3 and Last Glacial Maximum (LGM), where several millennial and plurimillennial scale climatic oscillation are observed. For these reasons it is possible suppose that dust transport mechanisms towards TD and DC were the same during the last glacial period. However, MIS 4 in TD ice core was lower, both in concentration and flux, compared to EDC. Likely, during LGM, very cool air masses extended above the Antarctic plateau, even in TD area, have created a subsidence condition which haven't allowed the cyclonic perturbation to penetrate the interior of Antarctica continent. In fact, the Polar Front has moved towards lower latitude in that period, maintaining far away the disturbances. These preliminary observations suggest that the coupling between inner sites such as EDC and the Talos Dome

  15. A high-resolution, absolute-dated deglacial speleothem record of Indian Ocean climate from Socotra Island, Yemen

    NASA Astrophysics Data System (ADS)

    Shakun, Jeremy D.; Burns, Stephen J.; Fleitmann, Dominik; Kramers, Jan; Matter, Albert; Al-Subary, Abdulkarim

    2007-07-01

    Stalagmite M1-5 from Socotra Island, Yemen in the northwest Indian Ocean provides a robust, high-resolution paleoclimate record from ˜ 27.4-11.1 ka based on 717 stable isotope and 28 230Th measurements. Variations in M1-5 oxygen isotope ratios ( δ18O) are interpreted to be primarily driven by an amount effect related to changes in the mean position and/or intensity of convection of the intertropical convergence zone, the island's only source of precipitation. The M1-5 δ18O time series is strongly correlated to the Greenland ice cores, similar to an older Socotra speleothem deposited from 53-40 ka [S.J. Burns, D. Fleitmann, A. Matter, J. Kramers, A. Al-Subbary, Indian Ocean climate and an absolute chronology over Dansgaard/Oeschger events 9 to 13, Science 301 (2003) 1365-1367], indicating that a North Atlantic-Indian Ocean cold-dry/warm-wet teleconnection persisted through the end of the last glacial period. Peak aridification occurred at ˜ 23 ka and a gradual increase in moisture thereafter was interrupted by an abrupt drying event at ˜ 16.4 ka, perhaps related to Heinrich event 1. Indian Ocean rainfall increased dramatically during the Bølling period and then decreased continuously and gradually through the Allerød and Younger Dryas. The Holocene began abruptly with increased precipitation at 11.4 ka and was followed by a major but short-lived drying during the Preboreal Oscillation at ˜ 11.2 ka. M1-5 is highly correlated to the Dongge Cave record from 15.5-11 ka, suggesting much of the Indian Ocean monsoon region responded similarly to the major climate changes of the last deglaciation. The transitions into the Younger Dryas and to a lesser extent the Bølling are remarkably gradual in M1-5, as they are in all other Asian speleothem records, occurring over several centuries. These gradual transitions are in striking contrast to high-resolution records from around the North Atlantic basin where the transitions are extremely abrupt and generally occur in

  16. A Reprocessed and Bias-Corrected High-Resolution Satellite Derived Precipitation Record Covering the Entire TRMM/GPM Era

    NASA Astrophysics Data System (ADS)

    Xie, P.; Joyce, R.; Sun, F.; Wu, S.; Yarosh, Y.; Yoo, S.

    2013-12-01

    CMORPH global high-resolution satellite precipitation estimates have been reprocessed and bias-corrected for a 15-year period from January 1998 to the present to cover the entire TRMM/GPM era. As the first step of this project, the CMORPH estimates have been extended backward in time from the December 2002 operational initiation to January 1998 and reprocessed from 2003 to present using the most recent passive microwave (PMW) retrieval algorithm version from all available low earth orbiters and infrared (IR) observations from geostationary platforms. The reprocessed CMORPH precipitation estimates consist of a 15-year homogeneous record of high-resolution precipitation on an 8kmx8km and 30-min resolution covering the globe from 60oS-60oN. Bias correction is then performed for the raw CMORPH over the entire data period from 1998 to the present. Over land, the bias in the raw CMORPH is removed by matching the probability density function (PDF) of the CMORPH with that of the CPC unified daily gauge analysis in two sequential steps. Bias in the raw CMORPH is first removed using PDF tables established for each 0.25olat/lon grid box and for each calendar day using co-located CMORPH - gauge data pairs collected over a sliding window of +/-15 days centered at the target calendar day over a 15-year period from 1998 to 2012 and over a spatial domain centering at the target grid box. The output of this first step is then calibrated against the daily gauge analysis using PDF tables established using data over a 30-day period ending at the target day to remove year-to-year variations of the CMORPH bias. Over ocean, the raw CMORPH satellite estimates are calibrated against a long-term precipitation analysis (pentad GPCP) to ensure temporal homogeneity for climate applications. The high-resolution CMORPH precipitation estimates are integrated to a time / space resolution of pentad / 2.5olat/lon to be compared against the pentad GPCP precipitation analysis. Ratio computed between

  17. High resolution in-vivo imaging of skin with full field optical coherence tomography

    NASA Astrophysics Data System (ADS)

    Dalimier, E.; Bruhat, Alexis; Grieve, K.; Harms, F.; Martins, F.; Boccara, C.

    2014-03-01

    Full-field OCT (FFOCT) has the ability to provide en-face images with a very good axial sectioning as well as a very high transverse resolution (about 1 microns in all directions). Therefore it offers the possibility to visualize biological tissues with very high resolution both on the axial native view, and on vertical reconstructed sections. Here we investigated the potential dermatological applications of in-vivo skin imaging with FFOCT. A commercial FFOCT device was adapted for the in-vivo acquisition of stacks of images on the arm, hand and finger. Several subjects of different benign and pathological skin conditions were tested. The images allowed measurement of the stratum corneum and epidermis thicknesses, measurement of the stratum corneum refractive index, size measurement and count of the keratinocytes, visualization of the dermal-epidermal junction, and visualization of the melanin granules and of the melanocytes. Skins with different pigmentations could be discriminated and skin pathologies such as eczema could be identified. The very high resolution offered by FFOCT both on axial native images and vertical reconstructed sections allows for the visualization and measurement of a set of parameters useful for cosmetology and dermatology. In particular, FFOCT is a potential tool for the understanding and monitoring of skin hydration and pigmentation, as well as skin inflammation.

  18. High-resolution elemental records of Glycymeris glycymeris (Bivalvia) shells from the Iberian upwelling system: Ontogeny and environmental control

    NASA Astrophysics Data System (ADS)

    Freitas, Pedro; Richardson, Christopher; Chenery, Simon; Butler, Paul; Reynolds, David; Gaspar, Miguel; Scourse, James

    2015-04-01

    The great potential of bivalve shells as a high-resolution geochemical proxy archive of environmental conditions at the time of growth has been known for several decades. The elemental composition of bivalve shells has been studied with the purpose of reconstructing environmental conditions: e.g. seawater temperature (Sr and Mg), primary productivity (Li, Mn, Mo and Ba), redox conditions (Mn and Mo), terrigenous inputs (Li) and pollution (Cu, Zn, Cd and Pb). However, the interpretation of such records remains extremely challenging and complex, with processes affecting element incorporation in the shell (e.g. crystal fabrics, organic matrix, shell formation mechanisms and physiological processes) and the influence of more than one environmental parameter affecting elemental composition of bivalve shells. Nevertheless, bivalve shells remain an underused source of information on environmental conditions, with the potential to record high-resolution (sub-weekly to annually), multi-centennial time series of geochemical proxy data. The relatively long-lived bivalve (>100 years) Glycymeris glycymeris occurs in coastal shelf seas of Europe and North West Africa and is a valid annually resolved sclerochronological archive for palaeonvironmental reconstructions. The temporal framework provided by absolute annually dated shell material makes Glycymeris glycymeris a valuable, albeit unexplored, resource for investigating sub-annually resolved geochemical proxies. We present a first evaluation on the potential of Ba, P and U, the latter two elements rarely studied in bivalves, in Glycymeris glycymeris shells to record variations in the environmental conditions, respectively primary productivity, dissolved inorganic phosphorus and carbonate ion concentration/pH. High-resolution (31 to 77 samples per year) profiles of elemental/Ca ratios (E/Ca) over four years of growth (2001 to 2004) were obtained by LA-ICP-MS on two shells (13 and 16 years old) live-collected in 2010 at 30 m

  19. A Boreal high-resolution composite carbon isotope record of the Albian to Turonian interval from the North German Basin

    NASA Astrophysics Data System (ADS)

    Bornemann, André; Erbacher, Jochen; Huck, Stefan; Heimhofer, Ulrich

    2017-04-01

    We present a potentially complete, six-hundred-meters-thick composite record of high-resolution bulk-rock carbon isotope data from northern Germany covering the topmost Lower Albian to Lower Turonian interval. The established composite record consists of more than 1700 measurements ana-lyzed from seven drill sites including the Kirchrode I and II cores (Nebe, 1999; Fenner, 2001), the Anderten I and II cores (Bornemann et al., 2017), two industrial cores Wunstorf 2011/2 and 2011/8 as well as the Wunstorf research core (Voigt et al., 2008). In the central North German Basin the Albian is represented by a several hundred meters-thick succession of clays and clayey marls, whereas the Lower Cenomanian is characterized by the transition from clayey to chalky sedimentation. The latter prevailed during the remaining Cenomanian. The top of the studied succession is marked by the prominent black shales of the Cenomanian-Turonian boundary event (CTBE). The isotope records display the carbon isotope expression of the Oceanic Anoxic Events 1d and 2 as well as the Lower and Mid-Cenomanian Events (LCE, MCE). The applied integrat-ed approach of high-resolution chemostratigraphy and revised biostratigraphy gives way for a substantial improvement of the Boreal Cretaceous stratigraphy. Fenner, J. (2001) The Kirchrode I and II boreholes: technical details and evidence on tectonics, and the palaeoceanographic development during the Albian. Palaeogeogr. Palaeoclimatol. Palaeoecol., 174, 33-65. Nebe, D. (1999) Zyklenuntersuchungen an unterkretazischen Sedimenten in NW-Deutschland - Nachweisbarkeit von Milankovitch-Zyklen [PhD thesis, Ruhr-Univ. Bochum]. Bornemann, A., Erbacher, J., Heldt, M., Kollaske, T., Lübke, N., Huck, S., Vollmar, N.M., Wilmsen, M. (2017) The Albian-Cenomanian transition and Oceanic Anoxic Event 1d - an example from the Boreal Realm. Sedimentol. [doi:10.1111/sed.12347]. Voigt, S., Erbacher, J., Mutterlose, J., Weiss, W., Westerhold, T., Wiese, F., Wilmsen, M

  20. A conceptual design for a Cassegrain-mounted high-resolution optical spectrograph for large-aperture telescopes

    NASA Astrophysics Data System (ADS)

    Froning, Cynthia S.; Osterman, Steven; Burgh, Eric; Beasley, Matthew; Scowen, Paul; Veach, Todd; Jordan, Steven; Ebbets, Dennis; Lieber, Michael; deCino, James; Castilho, Bruno Vaz; Gneiding, Clemens; César de Oliveira, Antonio

    2013-09-01

    We present a conceptual design for a high-resolution optical spectrograph appropriate for mounting at Cassegrain on a large aperture telescope. The design is based on our work for the Gemini High Resolution Optical Spectrograph (CUGHOS) project. Our design places the spectrograph at Cassegrain focus to maximize throughput and blue wavelength coverage, delivering R=40,000 resolving power over a continuous 320-1050 nm waveband with throughputs twice those of current instruments. The optical design uses a two-arm, cross-dispersed echelle format with each arm optimized to maximize efficiency. A fixed image slicer is used to minimize optics sizes. The principal challenge for the instrument design is to minimize flexure and degradation of the optical image. To ensure image stability, our opto-mechanical design combines a cost-effective, passively stable bench employing a honeycomb aluminum structure with active flexure control. The active flexure compensation consists of hexapod mounts for each focal plane with full 6-axis range of motion capability to correct for focus and beam displacement. We verified instrument performance using an integrated model that couples the optical and mechanical design to image performance. The full end-to-end modeling of the system under gravitational, thermal, and vibrational perturbations shows that deflections of the optical beam at the focal plane are <29 μm per exposure under the worst case scenario (<10 μm for most orientations), with final correction to 5 μm or better using open-loop active control to meet the stability requirement. The design elements and high fidelity modeling process are generally applicable to instruments requiring high stability under a varying gravity vector.

  1. High-resolution record of Early to Middle Miocene climate variability from Site 1195, Marion Plateau, NE Queensland margin

    NASA Astrophysics Data System (ADS)

    Lowery, C.; Browning, E.; Leckie, R. M.; John, C. M.

    2012-12-01

    Constraining and quantifying eustatic variations has been a priority for stratigraphers since the publication of the first global sea level curves by the Exxon research group in the late 1970s. Quantifying the glacio-eustatic component of sea level signals has become a greater focus as more recent work has demonstrated that far-field effects like ice-sheet gravitation and isostasy imprint on the "true" eustatic signal of waxing and waning ice sheets so that coeval signals from disparate sites may show significantly different local sea level variations. To this end, the Miocene sediments on the Marion Plateau, a drowned carbonate platform on the Queensland margin of Australia, were drilled by ODP Leg 194 to provide an independent, southern hemisphere test of the sea level record of the New Jersey Margin of North America, the most complete and oft-cited record of Cenozoic sea level variability. A high-resolution record is critical to compare sea level variations across hemispheres. Natural Gamma Ray (NGR) logs of core holes can provide a complete, high-resolution record independent of any problems with core recovery or sampling interval. We here present a NGR record of ODP Site 1195 tied to recently completed nannofossil assemblage data, planktic/benthic foraminiferal ratios, sedimentary particle counts, and benthic foraminifera stable isotopes. Peaks in glauconite and clay content correspond to peaks in NGR. The largest of these peaks correspond to sequence boundaries (lowstands), as glauconite accumulates during periods of low sedimentation along this carbonate-dominated margin. These sequence boundaries, in turn, are each associated with marine oxygen isotope events ("Mi Events") and correlate to sequences on the New Jersey margin, the Gulf of Papua, Great Australian Bight, and McMurdo Sound, indicating that these sequences are eustatically controlled. Although sedimentary particle counts only show strong peaks of glauconite at sequence boundaries, the NGR record

  2. Resolving seasonality in tropical trees: multi-decade, high-resolution oxygen and carbon isotope records from Indonesia and Thailand

    NASA Astrophysics Data System (ADS)

    Poussart, Pascale F.; Evans, Michael N.; Schrag, Daniel P.

    2004-02-01

    Dendrochronological techniques have found limited applications in the tropics because of invisible or indistinct banding in wood. The seasonal cycles of rainfall and relative humidity in these regions, while not sufficient to produce distinct visible rings, may still generate seasonal signals in the oxygen isotopic composition of tree cellulose which can be used for climate reconstruction and chronology. We explore this approach using trees from Indonesia and Thailand, from three different families. Multi-decade δ 18O records from Javanese cross-dated teak rings and bomb radiocarbon-dated Suar wood lacking visible rings demonstrate the reproducibility of the signal between trees grown at the same locality as well as from wider geographical regions. These results confirm predictions that the trees oxygen isotopic signatures reflect an external climatic forcing. High-resolution δ 18O records reveal large seasonal cycles: up to 4‰ for Javanese Suar samples and up to 18‰ for a Thai Podocarpus sample. We show that the six δ 18O and δ 13C cycles measured on a Podocarpus match the number of growth years for the period spanning the time of wounding and cutting of the wedge section. This result demonstrates that the isotopic cycles found in this tree with indistinct annual rings are indeed seasonal and could be used for chronology. We present evidence that stable isotope chronologies of tropical trees also contain insights in tropical tree physiology and growth dynamics.

  3. Linking the North Atlantic to central Europe: a high-resolution Holocene tephrochronological record from northern Germany

    NASA Astrophysics Data System (ADS)

    van den Bogaard, Christel; Schmincke, Hans-Ulrich

    2002-01-01

    A high-resolution Holocene tephrochronology for northern Germany has been established based on systematic tephrostratigraphical analysis of three peat bogs. Microscopic volcanic ash layers have been traced and characterised petrographically and by the chemical composition of the glass shards. At least 37 ash horizons representing 16 different explosive volcanic eruptions have been identified and many can be correlated between the three sites, up to 100 km apart. The tephra layers can be related to Icelandic volcanic sources and some correlated to the eruptions of Askja 1875, Hekla 3, Hekla Selsund, Hekla 4 and Hekla 5, as well as to unspecified eruptions of Icelandic volcanic systems, e.g. Torfajökull. The source volcanoes for some tephra layers remain unidentified. Some tephra layers were known previously from the North Atlantic region (e.g. Sluggan, Glen Garry), others have not been recorded previously in the literature (e.g. microlite tephra). This study provides the first comprehensive Holocene tephrostratigraphical record for northern Germany, complementing the North Atlantic tephrostratigraphical dating framework, effectively extending it into central Europe. The study shows that Icelandic ash layers are even more widespread than hitherto thought.

  4. Retinal Structure of Birds of Prey Revealed by Ultra-High Resolution Spectral-Domain Optical Coherence Tomography

    PubMed Central

    Ruggeri, Marco; Major, James C.; McKeown, Craig; Knighton, Robert W.; Puliafito, Carmen A.

    2010-01-01

    Purpose. To reveal three-dimensional (3-D) information about the retinal structures of birds of prey in vivo. Methods. An ultra-high resolution spectral-domain optical coherence tomography (SD-OCT) system was built for in vivo imaging of retinas of birds of prey. The calibrated imaging depth and axial resolution of the system were 3.1 mm and 2.8 μm (in tissue), respectively. 3-D segmentation was performed for calculation of the retinal nerve fiber layer (RNFL) map. Results. High-resolution OCT images were obtained of the retinas of four species of birds of prey: two diurnal hawks (Buteo platypterus and Buteo brachyurus) and two nocturnal owls (Bubo virginianus and Strix varia). These images showed the detailed retinal anatomy, including the retinal layers and the structure of the deep and shallow foveae. The calculated thickness map showed the RNFL distribution. Traumatic injury to one bird's retina was also successfully imaged. Conclusions. Ultra-high resolution SD-OCT provides unprecedented high-quality 2-D and 3-D in vivo visualization of the retinal structures of birds of prey. SD-OCT is a powerful imaging tool for vision research in birds of prey. PMID:20554605

  5. Retinal structure of birds of prey revealed by ultra-high resolution spectral-domain optical coherence tomography.

    PubMed

    Ruggeri, Marco; Major, James C; McKeown, Craig; Knighton, Robert W; Puliafito, Carmen A; Jiao, Shuliang

    2010-11-01

    To reveal three-dimensional (3-D) information about the retinal structures of birds of prey in vivo. An ultra-high resolution spectral-domain optical coherence tomography (SD-OCT) system was built for in vivo imaging of retinas of birds of prey. The calibrated imaging depth and axial resolution of the system were 3.1 mm and 2.8 μm (in tissue), respectively. 3-D segmentation was performed for calculation of the retinal nerve fiber layer (RNFL) map. High-resolution OCT images were obtained of the retinas of four species of birds of prey: two diurnal hawks (Buteo platypterus and Buteo brachyurus) and two nocturnal owls (Bubo virginianus and Strix varia). These images showed the detailed retinal anatomy, including the retinal layers and the structure of the deep and shallow foveae. The calculated thickness map showed the RNFL distribution. Traumatic injury to one bird's retina was also successfully imaged. Ultra-high resolution SD-OCT provides unprecedented high-quality 2-D and 3-D in vivo visualization of the retinal structures of birds of prey. SD-OCT is a powerful imaging tool for vision research in birds of prey.

  6. Low voltage and high resolution phase modulator based on blue phase liquid crystals with external compact optical system.

    PubMed

    Yan, Jing; Xing, Yufei; Guo, Zhengbo; Li, Qing

    2015-06-15

    Liquid crystal phase modulators are emerging as a new technological advancement, since they can be used for a wide range of applications. To improve their performance, polymer stabilized blue phase liquid crystal (PS-BPLC) phase modulators with fast response time and accurate phase profile become a necessary. Here, we proposed a facile PS-BPLC phase modulator to achieve particularly low voltage and high resolution. By employing a specific external compact optical system setup, the driving voltage is reduced to 26.09V to obtain 2π phase change at the wavelength of 532 nm. An accurate numerical modeling is also conducted to provide a systematic investigation of the fringing electric field effect to the performance of high resolution PS-BPLC phase modulator. The wavefront distortion caused by the fringing electric field can be automatically compensated to generate accurate phase profile for fast response liquid crystal phase modulator. This work provides a new protocol to realize liquid crystal on silicon based fast response and high resolution phase modulator.

  7. High-resolution retinal imaging in young children using a handheld scanner and Fourier-domain optical coherence tomography.

    PubMed

    Gerth, Christina; Zawadzki, Robert J; Héon, Elise; Werner, John S

    2009-02-01

    To test the feasibility and applicability of a handheld probe for Fourier-domain optical coherence tomography (Fd-OCT) retinal imaging in infants and children. Thirty children ages 7 months to 9.9 years, with (10 of 30) or without (20 of 30) retinal pathology, were imaged with Fd-OCT. Imaging was performed under sedation in 10 of 30 children ages 7 months to 3.7 years. A high-resolution Fd-OCT system (axial resolution: 4.5 mum; acquisition speeds: 1000 A-scans/frame, 9 frames/second), constructed at the UC Davis Medical Center, in conjunction with a handheld scanner, was used for retinal imaging. Useful images were obtained from all selected patients. Image acquisition was possible in a conscious state in children as young as 3 years of age. All children tolerated the tests well. The most challenging situation for young children was the lack of an internal fixation target and the moving scanning line, which usually distracted them from a steady fixation. Despite these problems, image quality was comparable with scans previously obtained from an adult population. The flexible handheld scanner in association with high acquisition speed and high-resolution Fd-OCT allows retinal imaging in infants and children. This technology provides high-resolution documentation of retinal structure in a pediatric population for the first time.

  8. Reconstruction of climate dynamics in an Arctic fjord environment: evidence from a multi-proxy high resolution marine record.

    NASA Astrophysics Data System (ADS)

    MacLachlan, S. E.; Howe, J.

    2012-12-01

    The cryosphere is a crucial component of the Earth's climate system, and comprises sea ice, snow, glaciers, ice cap, ice shelves, river and lake ice, ice sheets and frozen ground. The cryosphere has shown ice growth and decay on many timescales associated both with 100,000 year ice age cycles and with shorter-term (<2000 yrs) variations such as the Younger Dryas and the Little Ice Age. Crucially the cyosphere acts as a barometer for climate change because it provides a visible means of assessing the impacts of recent climate warming. Coastal Arctic regions are particularly sensitive to climate change, and records of glacier fluctuations can be used to infer past climate. The western Svalbard margin is a climatically sensitive region presently influenced by the warm and saline Atlantic water of the West Spitsbergen Current. This current is the northernmost extension of the Norwegian Atlantic Current that transports significant quantities of heat northward, maintaining the seas west of the Svalbard shelf increasingly ice free. For the Svalbard area there are currently a number of low-resolution (centennial to multi-decadal) marine records that span the Holocene. Despite their low resolution, several studies have highlighted abrupt environmental shifts and fluctuating glacial conditions during the Holocene. A few low-resolution lake records and other sporadic terrestrial datasets also exist providing a limited insight into the terrestrial environmental changes over the last two millennia. We have generated the first sub-decadal resolution late Holocene climatic record, in order to determine the nature and timing of environmental changes across transient climate events at an unprecedented temporal scale for this region. XRF analyses provides the high-resolution data series, which has been integrated with sedimentological data to better define the environmental processes; thus providing the basis for the reconstruction of climate change in this glaciated fjordic

  9. High-resolution CCD imagers using area-array CCD's for sensing spectral components of an optical line image

    NASA Technical Reports Server (NTRS)

    Elabd, Hammam (Inventor); Kosonocky, Walter F. (Inventor)

    1987-01-01

    CCD imagers with a novel replicated-line-imager architecture are abutted to form an extended line sensor. The sensor is preceded by optics having a slit aperture and having an optical beam splitter or astigmatic lens for projecting multiple line images through an optical color-discriminating stripe filter to the CCD imagers. A very high resolution camera suitable for use in a satellite, for example, is thus provided. The replicated-line architecture of the imager comprises an area-array CCD, successive rows of which are illuminated by replications of the same line segment, as transmitted by respective color filter stripes. The charge packets formed by accumulation of photoresponsive charge in the area-array CCD are read out row by row. Each successive row of charge packets is then converted from parallel to serial format in a CCD line register and its amplitude sensed to generate a line of output signal.

  10. High Resolution deglacial monsoon δ18O record from a new stalagmite from the Kailash Cave, Central India

    NASA Astrophysics Data System (ADS)

    Allu C, Narayana; Pawan K, Gautam; Shraddha, Band; Madhusudan G, Yadava; Rengaswamy, Ramesh; Shen, Chuan-Chou

    2016-04-01

    High resolution δ18O and δ13C data from absolutely dated stalagmites have been useful for reconstructing the Asian monsoon variability (e.g., Yadava et al., 2004; Laskar et al., 2013; Allu et al., 2014; Lone et al., 2014; Sinha et al., 2015). However, many studies lack high resolution spatial and temporal records leaving significant gaps which need to be filled for a vivid understanding of monsoonal variability. We report here the first high resolution stalagmite δ18O isotope results during the last deglacial obtained from the Kailash cave located from the core monsoon region. The length of stalagmite was 480 mm, with an average diameter of 120 mm. The sample was cut for continuous micro milling at 400μm intervals along the growth axis (using new wave research micro-mill-101288) for the analyses of stable oxygen and carbon isotopes using a Delta V plus IRMS at the Physical Research Laboratory, Ahmedabad. The physical appearance of the sample section reveals very fine, straight and clear laminations from the top to 310 mm from below, which have thick laminae. U-Th dates obtained from a Thermo Fisher NEPTUNE multi-collector inductively coupled plasma mass spectrometer (MC-ICP-MS) at High-Precision Mass Spectrometry and Environment Change Laboratory (HISPEC), National Taiwan University, Taiwan (Shen et al., 2012) showed the record spanned ~2400 years from ~14.6 ka to ~12.2 ka. Linear Age-Depth model constructed from dates suggests that the sample grew for ~2.400 years from ~14.6 ka to ~12.2 ka with varying resolutions from ~6 months to ~8 years. Hendy's test from 8 distinct layers shows poor correlation between δ18O and δ13C suggesting the isotopic equilibrium conditions at the time of crystallization. δ18O and δ13C results appear to be cyclic in nature varying in the range from +0.37‰ to -6.07‰ and -1.59‰ to -10.59‰ respectively. Enriched δ18O in top portion represents poor monsoon during the onset of Younger Drayas. Later, the δ18O signals

  11. Visible light high-resolution imaging system for large aperture telescope by liquid crystal adaptive optics with phase diversity technique.

    PubMed

    Xu, Zihao; Yang, Chengliang; Zhang, Peiguang; Zhang, Xingyun; Cao, Zhaoliang; Mu, Quanquan; Sun, Qiang; Xuan, Li

    2017-08-30

    There are more than eight large aperture telescopes (larger than eight meters) equipped with adaptive optics system in the world until now. Due to the limitations such as the difficulties of increasing actuator number of deformable mirror, most of them work in the infrared waveband. A novel two-step high-resolution optical imaging approach is proposed by applying phase diversity (PD) technique to the open-loop liquid crystal adaptive optics system (LC AOS) for visible light high-resolution adaptive imaging. Considering the traditional PD is not suitable for LC AOS, the novel PD strategy is proposed which can reduce the wavefront estimating error caused by non-modulated light generated by liquid crystal spatial light modulator (LC SLM) and make the residual distortions after open-loop correction to be smaller. Moreover, the LC SLM can introduce any aberration which realizes the free selection of phase diversity. The estimating errors are greatly reduced in both simulations and experiments. The resolution of the reconstructed image is greatly improved on both subjective visual effect and the highest discernible space resolution. Such technique can be widely used in large aperture telescopes for astronomical observations such as terrestrial planets, quasars and also can be used in other applications related to wavefront correction.

  12. Using electrons as a high-resolution probe of optical modes in individual nanowires.

    PubMed

    Arslan, Ilke; Hyun, Jerome K; Erni, Rolf; Fairchild, Michael N; Hersee, Stephen D; Muller, David A

    2009-12-01

    While nanowires show increasing promise for optoelectronic applications, probing the subwavelength details of their optical modes has been a challenge with light-based techniques. Here we report the excitation of dielectric optical waveguide modes in a single GaN nanowire using transition radiation generated by a 1 nm diameter electron beam. This spatially resolved study opens important gateways to probing the optical modes of more complex nanostructures, fundamental for optimization of optoelectronic device performance.

  13. A high-resolution compact optical true-time delay beamformer using fiber Bragg grating and highly dispersive fiber

    NASA Astrophysics Data System (ADS)

    Gao, Xinlu; Huang, Shanguo; Wei, Yongfeng; Gao, Chao; Zhou, Jing; Zhang, Hanyi; Gu, Wanyi

    2014-10-01

    A high resolution optical true-time delay (OTTD) beamformer constructed by fiber Bragg grating (FBG) and highly dispersive fiber (HDF) is presented. It can produce the true time delay with the resolution of 1 ps. Besides the proposed system has compact structure and light weight even when a large number of antenna elements are present in a practical antenna array, this is because the used FBG fibers and HDFs are short and independent of the antenna element number. Theoretical analysis and numerical simulations are made. Proof-of-concept experiment results that demonstrate the feasibility of the system are presented.

  14. High-resolution quantitative determination of dielectric function by using scattering scanning near-field optical microscopy

    PubMed Central

    Tranca, D. E.; Stanciu, S. G.; Hristu, R.; Stoichita, C.; Tofail, S. A. M.; Stanciu, G. A.

    2015-01-01

    A new method for high-resolution quantitative measurement of the dielectric function by using scattering scanning near-field optical microscopy (s-SNOM) is presented. The method is based on a calibration procedure that uses the s-SNOM oscillating dipole model of the probe-sample interaction and quantitative s-SNOM measurements. The nanoscale capabilities of the method have the potential to enable novel applications in various fields such as nano-electronics, nano-photonics, biology or medicine. PMID:26138665

  15. Atmospheric decadal variability from high-resolution Dome C ice core records of aerosol constituents beyond the Last Interglacial

    NASA Astrophysics Data System (ADS)

    Bigler, Matthias; Röthlisberger, Regine; Lambert, Fabrice; Wolff, Eric W.; Castellano, Emiliano; Udisti, Roberto; Stocker, Thomas F.; Fischer, Hubertus

    2010-01-01

    Along the EPICA Dome C ice core, we measured concentrations of different water-soluble aerosol constituents and deduced total depositional flux records. Here we present high-resolution sodium, calcium, ammonium and nitrate data covering the last 173,000 years. The first three of these species are passive tracers and reveal source and long-range transport changes whereas nitrate is deposited reversibly. However, it can be used to check isotope-derived accumulation rate estimates, a prerequisite to calculate total depositional fluxes. During the last two transitions from glacial to interglacial periods, changes in the total depositional flux differ strongly for different aerosol species. The largest changes are observed in the terrestrial aerosol proxy non-sea salt calcium, only moderate changes occur in the marine sea salt indicator sodium, while ammonium, a proxy for marine bioproductivity, remains rather constant. In agreement with previous studies, we find that only considerable glacial-interglacial changes at both, the terrestrial and the marine sea salt aerosol source can explain the observed pronounced changes. The unprecedented high-resolution of our data allows for the first time the examination of decadal variability back to the penultimate glacial period. On the one hand, we find occasional fast shifts occurring within a few years; here we present such an event in the calcium record from the penultimate glacial period. On the other hand, we examine variation coefficients and pairwise correlation coefficients, both determined in 200-year windows. They generally reveal only moderate changes. During glacial periods, slightly lower variation coefficients are found, concurrent with slightly higher correlation coefficients, which points to a more uniform and stronger coupled atmospheric long-range transport of the different aerosol species to the East Antarctic Plateau and less influence of cyclonic activities during cold periods. The opposite is observed for

  16. Non-Linear Response to Holocene Insolation Forcing Recorded by High-Resolution Lake Sediment Records Across Iceland

    NASA Astrophysics Data System (ADS)

    Geirsdottir, A.; Miller, G. H.; Axford, Y.

    2009-12-01

    Many Icelandic lakes have sedimentation rates in excess of 1 m ka-1 throughout the Holocene. Such high rates offer the potential for decadally resolved (or better) records of environmental change at this sensitive North Atlantic site. Abundant well-defined tephra provide a secure geochronology. The fidelity of the common climate proxies biogenic silica (BSi) and total organic carbon (TOC), was tested by comparing these proxies in three lakes with very different catchment characteristics. Hestvatn (HST, 60 m deep) in southern Iceland receives overflow from a large river originating in the glaciated highlands of central Iceland, whereas the nearby lake Vestra Gislholtsvatn (VGHV, 15 m deep) has a small, low elevation catchment without glaciers. Haukadalsvatn (HAK, 42 m deep), in northwestern Iceland, has a large, high relief catchment. The BSi record from HAK has been shown to reflect April-May temperatures, with BSi highest when spring temperatures are at their maximum. The first- and second-order trends in BSi are similar in all three lakes for most of the Holocene. This supports the contention that BSi reflects primary productivity, and is less influenced by changes in sedimentation rate. In all three lakes, BSi reaches a maximum value shortly after 8 ka, and then declines gradually toward present, reflecting a relatively late Holocene thermal maximum, potentially due to the influence of meltwater from the lingering Laurentide Ice Sheet. A steady reduction in summer insolation determines this first-order trend towards lower BSi through the middle and late Holocene. Large, abrupt departures from the overall decrease in BSi characterize all three records after 8 ka. Following each rapid BSi decrease, BSi usually exhibits a step-function change, re-equilibrating at a lower BSi value. Some of the strongest departures (ca. 6 ka, 4 to 4.5 ka and ca. 3 ka) may be related to Icelandic volcanism, but the lack of a full recovery to pre-existing values after the eruptions

  17. High Resolution Time Series Observations of Bio-optical and Physical Variability in the Arabian Sea

    DTIC Science & Technology

    1997-09-30

    Dynamics Program activities in the region. The Arabian Sea presents a very unique environment for study of bio -optical and physical processes and their...and biology of the upper layer. Thus, dynamical ranges in measured properties are great, enabling us to apply and test time dependent interdisciplinary models relevant to bio -optical properties and carbon fluxes.

  18. Multimodal three-dimensional imaging with isotropic high resolution using optical projection tomography

    NASA Astrophysics Data System (ADS)

    Miao, Qin; Rahn, J. Richard; Bryant, Ryland C.; Lancaster, Christy A.; Tourovskaia, Anna; Neumann, Thomas; Seibel, Eric J.; Nelson, Alan C.

    2009-02-01

    The optical projection tomography microscope (OPTM) is an optical microscope that acquires focus-invariant images from multiple views of single cells. Although the depth of field of the objective is short, it can be extended by scanning the objective's focal plane. This extended depth of field image is similar to a projection in conventional X-ray CT. Samples flow through a microcapillary tube filled with optical gel. Optical distortion is minimized by matching refractive index of optical gel and tube. Multiple projection images are taken by rotating the microcapillary tube with sub-micron mechanical precision. After these pseudoprojection images are further aligned, computed tomography methods are then applied to the images to create a 3D reconstruction with isometric resolution of 0.35 microns. Three-dimensional reconstructed images of fluorescent microspheres and cells are shown.

  19. Very high resolution optical transition radiation imaging system: Comparison between simulation and experiment

    NASA Astrophysics Data System (ADS)

    Bolzon, B.; Aryshev, A.; Aumeyr, T.; Boogert, S.; Karataev, P.; Kruchinin, K. O.; Lefevre, T.; Mazzoni, S.; Nevay, L.; Shevelev, M.; Terunuma, N.; Urakawa, J.; Welsch, C. P.

    2015-08-01

    Optical transition radiation (OTR) has become a commonly used method for 2D beam imaging measurements. In the Accelerator Test Facility 2 (ATF2) at KEK, beam sizes smaller than the OTR point spread function have been measured. Simulations of the OTR imaging system have been performed using the ZEMAX software to study the effects of optical errors such as aberrations, diffraction, and misalignments of optical components. This paper presents a comparison of simulations of the OTR point spread function with experimental data obtained at ATF2. It shows how the quantification and control of optical errors impacts on optimizing the resolution of the system. We also show that the OTR point spread function needs to be predicted accurately to optimize any optical system and to predict the error made on measurement.

  20. Two phases of the Holocene East African Humid Period: Inferred from a high-resolution geochemical record off Tanzania

    NASA Astrophysics Data System (ADS)

    Liu, Xiting; Rendle-Bühring, Rebecca; Kuhlmann, Holger; Li, Anchun

    2017-02-01

    During the Holocene, the most notably climatic change across the African continent is the African Humid Period (AHP), however the pace and primary forcing for this pluvial condition is still ambiguous, particularly in East Africa. We present a high-resolution marine sediment record off Tanzania to provide insights into the climatic conditions of inland East Africa during the Holocene. Major element ratios (i.e., log-ratios of Fe/Ca and Ti/Ca), derived from X-Ray Fluorescence scanning, have been employed to document variations in humidity in East Africa. Our results show that the AHP is represented by two humid phases: an intense humid period from the beginning of the Holocene to 8 ka (AHP I); and a moderate humid period spanning from 8 to 5.5 ka (AHP II). On the basis of our geochemical record and regime detection, the termination of the AHP initiated at 5.5 ka and ceased around 3.5 ka. Combined with other paleoclimatic records around East Africa, we suggest that the humid conditions in this region responded to Northern Hemisphere (NH) summer insolation. The AHP I and II might have been related to an eastward shift of the Congo Air Boundary and warmer conditions in the western Indian Ocean, which resulted in additional moisture being delivered from the Atlantic and Indian Oceans during the NH summer and autumn, respectively. We further note a drought event throughout East Africa north of 10°S around 8.2 ka, which may have been related to the southward migration of the Intertropical Convergence Zone in response to the NH cooling event.

  1. Changes in black carbon deposition to Antarctica from two high-resolution ice core records, 1850-2000 AD

    NASA Astrophysics Data System (ADS)

    Bisiaux, M. M.; Edwards, R.; McConnell, J. R.; Curran, M. A. J.; Van Ommen, T. D.; Smith, A. M.; Neumann, T. A.; Pasteris, D. R.; Penner, J. E.; Taylor, K.

    2012-05-01

    Refractory black carbon aerosols (rBC) emitted by biomass burning (fires) and fossil fuel combustion, affect global climate and atmospheric chemistry. In the Southern Hemisphere (SH), rBC is transported in the atmosphere from low- and mid-latitudes to Antarctica and deposited to the polar ice sheet preserving a history of emissions and atmospheric transport. Here, we present two high-resolution Antarctic rBC ice core records drilled from the West Antarctic Ice Sheet divide and Law Dome on the periphery of the East Antarctic ice sheet. Separated by ~3500 km, the records span calendar years 1850-2001 and reflect the rBC distribution over the Indian and Pacific ocean sectors of the Southern Ocean. Concentrations of rBC in the ice cores displayed significant variability at annual to decadal time scales, notably in ENSO-QBO and AAO frequency bands. The delay observed between rBC and ENSO variability suggested that ENSO does not directly affect rBC transport, but rather continental hydrology, subsequent fire regimes, and aerosol emissions. From 1850 to 1950, the two ice core records were uncorrelated but were highly correlated from 1950 to 2002 (cross-correlation coefficient at annual resolution: r = 0.54, p < 0.01) due to a common decrease in rBC variability. The decrease in ice-core rBC from the 1950s to late 1980s displays similarities with inventories of SH rBC grass fires and biofuel emissions, which show reduced emission estimates over that period.

  2. Century-scale high-resolution black carbon records in sediment cores from the South Yellow Sea, China

    NASA Astrophysics Data System (ADS)

    Xu, Xiaoming; Hong, Yuehui; Zhou, Qianzhi; Liu, Jinzhong; Yuan, Lirong; Wang, Jianghai

    2017-04-01

    Black carbon (BC) has received increasing attention in the last 20 years because it is not only an absorbent of toxic pollutants but also a greenhouse substance, preserving fire-history records, and more importantly, acting as an indicator of biogeochemical cycles and global changes. By adopting an improved chemothermal oxidation method (WXY), this study reconstructed the century-scale high-resolution records of BC deposition from two fine-grained sediment cores collected from the Yellow Sea Cold Water Mass in the South Yellow Sea. The BC records were divided into five stages, which exhibited specific sequences with three BC peaks at approximately 1891, 1921, and 2007 AD, representing times at which the first heavy storms appeared just after the termination of long-term droughts. The significant correlation between the times of the BC peaks in the cores and heavy storms in the area of the Huanghe (Yellow) River demonstrated that BC peaks could result from markedly strengthened sedimentation due to surface runoff, which augmented the atmospheric deposition. Stable carbon isotope analysis indicated that the evident increase in carbon isotope ratios of BC in Stage 5 might have resulted from the input of weathered rock-derived graphitic carbon cardinally induced by the annual anthropogenic modulation of water-borne sediment in the Huanghe River since 2005 AD. Numerical calculations demonstrated that the input fraction of graphitic carbon was 22.97% for Stage 5, whereas no graphitic carbon entered during Stages 1 and 3. The obtained data provide new and important understanding of the source-sink history of BC in the Yellow Sea.

  3. A High-Resolution Mid- to Late-Holocene Sea-Surface Temperature Record from the Subtropics, Baja California, Mexico

    NASA Astrophysics Data System (ADS)

    Coombs, M.; Herguera, J. C.; Lea, D. W.

    2004-12-01

    San Lazaro basin provides a unique environment in which to study paleoclimate because it lies at the intersection of the cooler California Current that originates to the north and the warmer Pacific tropical current that originates to the south. Studies in this basin will provide insight into the varying strength of these currents at decadal and centennial resolutions. In addition, the present-day basin is suboxic (<3 uM/kg), and laminations throughout the top ˜6 m of ocean floor sediments indicate that it may have been suboxic for the past ˜6.5 ky. Suboxia is advantageous in the study of high-resolution paleoclimate records, as lower levels of oxygen inhibit the activity of sediment-disturbing organisms that might smooth climate records. We will present parts of a new decadal-resolution sea-surface temperature (SST) record from San Lazaro (AKA Soledad) basin, Baja California, Mexico. We estimate SST using Mg/Ca and d18O data measured on surface-dwelling planktonic foraminifera Globigerinoides ruber and Globigerina bulloides from ocean-sediment giant box core MD02-2506C2. This 4.6-m core exhibits an ˜0.8 mm/yr sedimentation rate (van Geen et al., 2003) and represents the period ˜5,000 to ˜500 years BP. We sampled the core at 1-cm continuous (i.e., approximately decadal) resolution. Preliminary results indicate that Mg/Ca values for G. ruber range between 2.3 and 3.3 mmol/mol ( ˜20 and ˜24 deg C), with higher values characteristic of the mid-Holocene. Annual sea-surface temperatures for the period 1950 to 1990 ranged between ˜19 and ˜23 deg C (CalCOFI, COADS), suggesting that mid- to late-Holocene SST values were ˜1 deg C higher than the those of the last half century. We will compare our SST proxy results with d18O records from Santa Barbara basin (e.g., Kennett and Kennett, 2000); tree-ring records from Baja California (Bullock, 2003) and Southern California (De Nava et al., 1999); and pluvial (Davis, 2003) and packrat midden (Clark and Sankey, 1999

  4. High resolution 3D imaging of living cells with sub-optical wavelength phonons

    PubMed Central

    Pérez-Cota, Fernando; Smith, Richard J.; Moradi, Emilia; Marques, Leonel; Webb, Kevin F.; Clark, Matt

    2016-01-01

    Label-free imaging of living cells below the optical diffraction limit poses great challenges for optical microscopy. Biologically relevant structural information remains below the Rayleigh limit and beyond the reach of conventional microscopes. Super-resolution techniques are typically based on the non-linear and stochastic response of fluorescent labels which can be toxic and interfere with cell function. In this paper we present, for the first time, imaging of live cells using sub-optical wavelength phonons. The axial imaging resolution of our system is determined by the acoustic wavelength (λa = λprobe/2n) and not on the NA of the optics allowing sub-optical wavelength acoustic sectioning of samples using the time of flight. The transverse resolution is currently limited to the optical spot size. The contrast mechanism is significantly determined by the mechanical properties of the cells and requires no additional contrast agent, stain or label to image the cell structure. The ability to breach the optical diffraction limit to image living cells acoustically promises to bring a new suite of imaging technologies to bear in answering exigent questions in cell biology and biomedicine. PMID:27996028

  5. High resolution 3D imaging of living cells with sub-optical wavelength phonons

    NASA Astrophysics Data System (ADS)

    Pérez-Cota, Fernando; Smith, Richard J.; Moradi, Emilia; Marques, Leonel; Webb, Kevin F.; Clark, Matt

    2016-12-01

    Label-free imaging of living cells below the optical diffraction limit poses great challenges for optical microscopy. Biologically relevant structural information remains below the Rayleigh limit and beyond the reach of conventional microscopes. Super-resolution techniques are typically based on the non-linear and stochastic response of fluorescent labels which can be toxic and interfere with cell function. In this paper we present, for the first time, imaging of live cells using sub-optical wavelength phonons. The axial imaging resolution of our system is determined by the acoustic wavelength (λa = λprobe/2n) and not on the NA of the optics allowing sub-optical wavelength acoustic sectioning of samples using the time of flight. The transverse resolution is currently limited to the optical spot size. The contrast mechanism is significantly determined by the mechanical properties of the cells and requires no additional contrast agent, stain or label to image the cell structure. The ability to breach the optical diffraction limit to image living cells acoustically promises to bring a new suite of imaging technologies to bear in answering exigent questions in cell biology and biomedicine.

  6. Carbon cycle dynamics and solar activity embedded in a high-resolution 14C speleothem record from Belize, Central America

    NASA Astrophysics Data System (ADS)

    Lechleitner, Franziska A.; Breitenbach, Sebastian F. M.; McIntyre, Cameron; Asmerom, Yemane; Prufer, Keith M.; Polyak, Victor; Culleton, Brendan J.; Kennett, Douglas J.; Eglinton, Timothy I.; Baldini, James U. L.

    2015-04-01

    Speleothem 14C has recently emerged as a potentially powerful proxy for climate reconstruction. Several studies have highlighted the link between karst hydrology and speleothem 14C content, and a number of possible causes for this relationship have been proposed, such as dripwater flow dynamics in the karst and changes in soil organic matter (SOM) turnover time (e.g. Griffiths et al., 2012). Here we present a high resolution 14C record for a stalagmite (YOK-I) from Yok Balum cave in southern Belize, Central America. YOK-I grew continuously over the last 2000 years, and has been dated very precisely with the U-Th method (40 dates, mean uncertainty < 10 years). The excellent chronological control for this stalagmite allows us to calculate 14C activity (a14C) at the time of speleothem deposition (a14Cinit), as well as the dead carbon fraction (DCF), predominantly a measure of the reservoir effect introduced by limestone dissolution in the karst (Genty et al., 2001). Both records show striking similarities to atmospheric a14C (IntCal13) and reconstructions of solar activity and 14C production rate. We infer close coupling between cave environment and atmosphere, with minimal signal dampening, an observation supported by monitoring data (Ridley et al., in press). DCF fluctuates between approximately 10% and 16% over the entire record, with distinctly lower DCF values and higher a14Cinit during a period of reduced rainfall between ca. 700-1100 AD (linked to the Classic Maya Collapse). This behavior is consistent with observations made elsewhere, and suggests that DCF responds to karst hydrological variability, specifically open-closed system transitions. YOK-I a14Cinit typically lags atmospheric values by 10-100 cal years. A shorter lag appears to be linked to periods of drought, suggesting a response of SOM dynamics above the cave to rainfall reduction. Specifically, drought is inferred to lead to reduced bioproductivity and soil carbon turnover, lowering contributions

  7. Flexible miniature compound lens design for high-resolution optical coherence tomography balloon imaging catheter

    PubMed Central

    Fu, Henry L.; Leng, Yuxin; Cobb, Michael J.; Hsu, Kevin; Hwang, Joo Ha; Li, Xingde

    2009-01-01

    We report on a new optics design for an optical coherence tomography (OCT) balloon imaging catheter. The design involves a miniature compound gradient-index (GRIN) rod lens, which consists of a fiber optic mode-field reducer and relay rod lenses to achieve predictable high lateral resolution at a desired large working distance. The compound lens design significantly simplifies the engineering process for an OCT catheter and enables 3-D full circumferential cross sectional imaging of large luminal organs such as human esophagus. An as-designed OCT catheter is developed and demonstrated for real-time in vivo swine esophagus imaging in a 3-D spiral fashion. PMID:19123643

  8. Optics optimization in high-resolution imaging module with extended depth of field

    NASA Astrophysics Data System (ADS)

    Chen, Xi; Bakin, Dmitry; Liu, Changmeng; George, Nicholas

    2008-08-01

    The standard imaging lens for a high resolution sensor was modified to achieve the extended depth of field (EDoF) from 300 mm to infinity. In the module the raw sensor outputs are digitally processed to obtain high contrast images. The overall module is considered as an integrated computational imaging system (ICIS). The simulation results for illustrative designs with different amount of spherical aberrations are provided and compared. Based on the results of simulations we introduced the limiting value of the PSF Strehl ratio as the integral threshold criteria to be used during EDoF lens optimization. A four-element standard lens was modified within the design constraints to achieve the EDoF performance. Two EDoF designs created with different design methods are presented. The imaging modules were compared in terms of Strehl ratios, limiting resolution, modulation frequencies at 50% contrast, and SNR. The output images were simulated for EDoF modules, passed through the image processing pipeline, and compared against the images obtained with the standard lens module.

  9. High resolution imaging of impacted CFRP composites with a fiber-optic laser-ultrasound scanner.

    PubMed

    Pelivanov, Ivan; Ambroziński, Łukasz; Khomenko, Anton; Koricho, Ermias G; Cloud, Gary L; Haq, Mahmoodul; O'Donnell, Matthew

    2016-06-01

    Damage induced in polymer composites by various impacts must be evaluated to predict a component's post-impact strength and residual lifetime, especially when impacts occur in structures related to human safety (in aircraft, for example). X-ray tomography is the conventional standard to study an internal structure with high resolution. However, it is of little use when the impacted area cannot be extracted from a structure. In addition, X-ray tomography is expensive and time-consuming. Recently, we have demonstrated that a kHz-rate laser-ultrasound (LU) scanner is very efficient both for locating large defects and evaluating the material structure. Here, we show that high-quality images of damage produced by the LU scanner in impacted carbon-fiber reinforced polymer (CFRP) composites are similar to those produced by X-ray tomograms; but they can be obtained with only single-sided access to the object under study. Potentially, the LU method can be applied to large components in-situ.

  10. Object-based classification of earthquake damage from high-resolution optical imagery using machine learning

    NASA Astrophysics Data System (ADS)

    Bialas, James; Oommen, Thomas; Rebbapragada, Umaa; Levin, Eugene

    2016-07-01

    Object-based approaches in the segmentation and classification of remotely sensed images yield more promising results compared to pixel-based approaches. However, the development of an object-based approach presents challenges in terms of algorithm selection and parameter tuning. Subjective methods are often used, but yield less than optimal results. Objective methods are warranted, especially for rapid deployment in time-sensitive applications, such as earthquake damage assessment. Herein, we used a systematic approach in evaluating object-based image segmentation and machine learning algorithms for the classification of earthquake damage in remotely sensed imagery. We tested a variety of algorithms and parameters on post-event aerial imagery for the 2011 earthquake in Christchurch, New Zealand. Results were compared against manually selected test cases representing different classes. In doing so, we can evaluate the effectiveness of the segmentation and classification of different classes and compare different levels of multistep image segmentations. Our classifier is compared against recent pixel-based and object-based classification studies for postevent imagery of earthquake damage. Our results show an improvement against both pixel-based and object-based methods for classifying earthquake damage in high resolution, post-event imagery.

  11. High-resolution imaging of colonic mucosa using optical coherence tomography

    NASA Astrophysics Data System (ADS)

    Wang, Ruikang K.; Elder, James B.; Smith, Victoria

    2001-05-01

    We report further the capability of OCT to delineate the microstructures beneath the colonic tissue surface, and to discriminate the normal tissues form the diseased ones. The OCT system operating at central wavelength at 820 nm was used which has measured axial resolution of 12 microns in free space and transversal resolution at 16 microns. The tissue specimens were obtained form the patient in the theater, who diagnosed as bearing colonic cancer and underwent conventional operations in the Hospital, and imaged with the OCT within 0.5-1 hour of removal. More than 10 patients were studied. The result of this study indicates that the important clinical layers and features with depth down to 1~2.0 mm of the colonic mucosa could be clearly delineated with the OCT imaging, and their thickness correlates well with the histology. The OCT images are also able to differentiate the normal colonic mucosa from the diseased ones. In conclusion, OCT is capable of high- resolution in situ imaging of colonic microstructures, without the need for excisional biopsy.

  12. Triplet states in isotopically mixed anthracene crystals: High resolution optical spectroscopy

    NASA Astrophysics Data System (ADS)

    Port, H.; Rund, D.; Wolf, H. C.

    1981-08-01

    The triplet O,O transitions of guest and host in isotopically mixed anthracene crystals of various compositions (A- h10, 13C-monosubstituted A- h10, A- d1h9, A- d2hg in A- d10 and A- d10 in A- h10) have been investigated using high resolution laser excitation spectroscopy. The guest aggregate spectra have been studied in polarized light as a function of guest concentration up to 15%. The analyses allow us to identify the monomer, dimer and trimer lines. From the dimer splittings the dominant resonance pair interactions are dedu The comparison of different mixed crystal systems with guest levels below and above the host exciton band reveals that quasiresonance and superexchange corrections are of minor importance. The experimental resonance pair interactions are used to calculate the triplet exciton band structure of anthracen and the observed guest polarization behaviour is interpreted quantitatively by the Rashba effect. Finally, the lower Davydov component of the host is s and broadened with increasing guest concentration. The shift is discussed using a theoretical model of Lifshitz.

  13. High resolution stalagmite climate record from the Yucatán Peninsula spanning the Maya terminal classic period

    NASA Astrophysics Data System (ADS)

    Medina-Elizalde, Martín; Burns, Stephen J.; Lea, David W.; Asmerom, Yemane; von Gunten, Lucien; Polyak, Victor; Vuille, Mathias; Karmalkar, Ambarish

    2010-09-01

    The decline of the Classic Maya civilization was complex and geographically variable, and occurred over a ~ 150-year interval, known as the Terminal Classic Period (TCP, C.E. 800-950). Paleoclimate studies based on lake sediments from the Yucatán Peninsula lowlands suggested that drought prevailed during the TCP and was likely an important factor in the disintegration of the Classic Maya civilization. The lacustrine evidence for decades of severe drought in the Yucatán Peninsula, however, does not readily explain the long 150-year socio-political decline of the Classic Maya civilization. Here we present a new, absolute-dated, high-resolution stalagmite δ18O record from the northwest Yucatán Peninsula that provides a much more detailed picture of climate variability during the last 1500 years. Direct calibration between stalagmite δ18O and rainfall amount offers the first quantitative estimation of rainfall variability during the Terminal Classic Period. Our results show that eight severe droughts, lasting from 3 to 18 years, occurred during major depopulation events of Classic Maya city-states. During these droughts, rainfall was reduced by 52% to 36%. The number and short duration of the dry intervals help explain why the TCP collapse of the Mayan civilization occurred over 150 years.

  14. High-resolution depositional records of polycyclic aromatic hydrocarbons in the central continental shelf mud of the East China Sea

    SciTech Connect

    Zhigang Guo; Tian Lin; Gan Zhang; Zuosheng Yang; Ming Fang

    2006-09-01

    A well-placed {sup 210}Pb-dated sediment core extracted from the distal mud in the central continental shelf of the East China Sea is used to reconstruct the high-resolution atmospheric depositional record of polycyclic aromatic hydrocarbons (PAHs), believed to be transported mainly from China in the past 200 years due to the East Asian Monsoon. Total PAHs (TPAHs), based on the 16 USEPA priority PAHs, range from 27 in 1788 to 132 ng g{sup -1} in 2001. TPAH variation in the core reflects energy usage changes and follows closely the historical economic development in China. PAHs in the core are dominantly pyrogenic in source, i.e., they are mainly from the incomplete combustion of coal and biomass burning. Several individual PAHs suggest that contribution from incomplete petroleum combustion has increased during recent years. Analysis of the 2 + 3 ring and the 5 + 6 ring PAHs and principle component analysis provide more evidence in the change in the energy structure, especially after 1978 when China embarked on the 'Reform and Open' Policy, indicating the transformation from an agricultural to an industrial economy of China. The historical profile of PAH distribution in the study area is obviously different from the United States and Europe due to their difference in energy structure and economic development stages. 39 refs., 5 figs.

  15. Permanent Electronic Storage of Angiographic Images in the Catheterization Laboratory: High-Resolution and Real-Time Acquisition on Optical Laserdiscs.

    PubMed

    Azancot; Krone; Eiferman; Guermonprez; Metzger; Le Dref O; Steg; Bonan; Lesperance; Hudon; Friolo; Beaufils

    1997-03-01

    The use of electronic ÒfilmlessÓ media for long-term archiving of coronary angiograms has been impeded by the problems of image storage and data transfer among institutions. Although long-term analogue storage of the images is presently feasible, and much less costly than digital storage, processing has been limited to a 625 lines video format, not optimal for high quality images. We developed a bi-directional 1249/625 lines converter, able to store and to replay high resolution (1249 lines) video images, from Component Record Video (CRV) Optical Laser (Analogue) Videodisks. Image quality and medical relevance were evaluated five ways: 1) Outside experts compared 593 static images stored on CRV discs to the same images stored in a high-resolution digital format blinded to source of image. The four experts found no visual or medical difference in 98% of evaluated images and minor differences in the remainder. The differences in the remaining images were not consistent among experts. 2) Two of the experts also compared the enhanced CRV optically stored image to the image obtained on simultaneously recorded 35mm cine film, and found the enhanced CRV stored image to be superior or similar, but never inferior to the film image. 3) 90 representatives from 63 outside institutions compared images from both a digital hard drive and the enhanced CRV optical (analogue) storage displayed at a Windows based digital workstation. During the test they were blinded as to the source of the images. The representatives found no difference in image resolution, quality, diagnostic accuracy, and medical relevance. 4) We evaluated quantitative coronary angiography (QCA) on standard coronary test phantoms using enhanced CRV stored images digitally processed. The correlation of the enhanced CRV image to the actual size of the phantom vessels was similar to the results obtained in the literature from digitally stored images. 5) 78 arterial measurements ranging 0.65 to 4.85 mm were

  16. A novel high-resolution optical imaging modality: photo-magnetic imaging

    NASA Astrophysics Data System (ADS)

    Luk, Alex T.; Thayer, David; Lin, Yuting; Nouizi, Farouk; Gao, Hao; Gulsen, Gultekin

    2013-03-01

    We introduce an entirely new technique, termed Photo-Magnetic Imaging (PMI), which overcomes the limitation of pure optical imaging and provides optical absorption at MRI spatial resolution. PMI uses laser light to heat the medium under investigation and employs MR thermometry for the determination of spatially resolved optical absorption in the probed medium. A FEM-based PMI forward solver has been developed by modeling photon migration and heat diffusion in tissue to compare simulation results with measured MRI maps. We have successfully performed PMI using 2.5 cm diameter agar phantom with two low optical absorption contrast (x 4) inclusions under the ANSI limit. Currently, we are developing the PMI inverse solver and undertaking further phantom and in vivo experiments.

  17. Interrogating Biology with Force: Single Molecule High-Resolution Measurements with Optical Tweezers

    PubMed Central

    Capitanio, Marco; Pavone, Francesco S.

    2013-01-01

    Single molecule force spectroscopy methods, such as optical and magnetic tweezers and atomic force microscopy, have opened up the possibility to study biological processes regulated by force, dynamics of structural conformations of proteins and nucleic acids, and load-dependent kinetics of molecular interactions. Among the various tools available today, optical tweezers have recently seen great progress in terms of spatial resolution, which now allows the measurement of atomic-scale conformational changes, and temporal resolution, which has reached the limit of the microsecond-scale relaxation times of biological molecules bound to a force probe. Here, we review different strategies and experimental configurations recently developed to apply and measure force using optical tweezers. We present the latest progress that has pushed optical tweezers’ spatial and temporal resolution down to today’s values, discussing the experimental variables and constraints that are influencing measurement resolution and how these can be optimized depending on the biological molecule under study. PMID:24047980

  18. Research on the influence of the disturbance characteristics of the flywheel components on a high resolution optical satellite

    NASA Astrophysics Data System (ADS)

    Li, Lin; Zhou, Sitong; Kong, Lin; Xu, Jing; Wang, Dong

    2016-10-01

    In order to study the influence of flywheel micro vibration on the imaging of a high resolution optical satellite, the flywheel components disturbance model was established, and the flywheel components were tested. The analysis of the measured data shows that there is a series of harmonic at the first order frequency 50Hz, and a series of peaks around the 190Hz and 280Hz. The integration of the angular displacement response that was obtained by exerting the unit sine excitation on the satellite and the flywheel measured disturbance data shows that there is a lot of angular displacement harmonic response frequency in 40Hz 80Hz and 230Hz 280Hz, the maximum angular displacement resonance response amplitude is 2.739" along the vertical direction, the angular displacement resonance response amplitude is 2.617" at 245Hz and 2600rpm, and 0.5" magnitude harmonic amplitude around 245Hz. Flywheel micro vibration has a great influence on the high resolution optical satellite imaging quality. Suggestions on further research on micro vibration of flywheel are proposed.

  19. A Boreal high-resolution d13C-carb record of the Albian-Cenomanian transition from NW Germany

    NASA Astrophysics Data System (ADS)

    Bornemann, André; Erbacher, Jochen; Heldt, Matthias; Wilmsen, Markus; Lübke, Nathalie; Heimhofer, Ulrich

    2015-04-01

    . London Spec. Publ., 104, 11-24. Stoll, H.M., Schrag, D.P. (2000). High-resolution stable isotope records from the Upper Cretaceous rocks of Italy and Spain: Glacial episodes in a greenhouse planet? Geol. Soc. Am. Bull., 112, 308-319.

  20. Holocene water mass history off NE Greenland - A first high-resolution sediment record from the western Fram Strait

    NASA Astrophysics Data System (ADS)

    Zehnich, Marc; Palme, Tina; Spielhagen, Robert F.; Hass, H. Christian; Bauch, Henning A.

    2017-04-01

    While the Holocene history of the eastern Fram Strait seems well investigated, no high-resolution paleoenvironmental records were available from the western Fram Strait so far. A new sedimentary record, obtained during expedition PS93.1 (2015) of RV Polarstern on the outermost NE Greenland shelf, allows for the first time to reconstruct Holocene changes in near-surface salinities, temperature, stratification and water masses (polar waters vs. Atlantic Water), potentially related to variations of the freshwater and sea ice export from the Arctic Ocean. The 260 cm long sedimentary record from site PS93/025 (80.5°N, 8.5°W) was investigated for sediment composition, foraminifer contents, grain size variations (sortable silt) and the isotopic composition of planktic foraminifers. Radiocarbon datings reveal an age of 10.2 cal-ka for the core base and continuous sedimentation throughout most of the Holocene. The sediments are generally very fine-grained (<2% sand). The grain size record reveals a fining-upwards trend and sediments from <6.5 cal-ka consist of <0.5% coarse fraction. A comparison of foraminifer and coarse fraction abundances shows strong similarities. Apparently the contribution of coarse terrestrial material from iceberg transport was extremely low throughout the last 10.2 cal-ka. Foraminifer abundances (both planktic and benthic) are high in Early Holocene sediments until ca. 7 cal-ka and decrease rapidly thereafter. This is interpreted to reflect a relatively strong advection of Atlantic Water to the NW Fram Strait, which correlates well with similar findings on the eastern side of the Arctic Gateway. Sortable silt grain sizes are high (27-32 µm) in the older part of the record and gradually decrease between 7 cal-ka and 4 cal-ka. After ca. 4 cal-ka, sortable silt shows values of 20-22 µm and little variation. Considering also the grain-size distribution curves, we propose a decline of bottom current velocities on the outer NE Greenland shelf after 7

  1. Assessment of ultra-high resolution optical coherence tomography for monitoring tissue effects caused by laser photocoagulation of ex-vivo porcine retina

    NASA Astrophysics Data System (ADS)

    Steiner, Patrick; Enzmann, Volker; Wolf, Sebastian; Bossen, Anke; Meier, Christoph; Sznitman, Raphael

    2015-03-01

    Retinal laser photocoagulation is an established and successful treatment for a variety of retinal diseases. While being a valuable treatment modality, laser photocoagulation shows the drawback of employing high energy lasers which are capable of physically destroying the neural retina. For reliable therapy, it is therefore crucial to closely monitor the therapy effects caused in the retinal tissue. A depth resolved representation of optical tissue properties as provided by optical coherence tomography may provide valuable information about the treatment effects in the retinal layers if recorded simultaneously to laser coagulation. Therefore, in this work, the use of ultra-high resolution optical coherence tomography to represent tissue changes caused by conventional and selective retinal photocoagulation is investigated. Laser lesions were placed on porcine retina ex-vivo using a 577 nm laser as well as a pulsed laser at 527 nm built for selective treatment of the retinal pigment epithelium. Applied energies were varied to generate lesions best representing the span from under- to overtreatment. The lesions were examined using a custom-designed optical coherence tomography system with an axial resolution of 1.78 μm and 70 kHz Ascan rate. Optical coherence tomography scans included volume scans before and after irradiation, as well as time lapse scans (Mscan) of the lesions. Results show OCT lesion visibility thresholds to be below the thresholds of ophthalmoscopic inspection. With the ultra-high resolution OCT, 42% - 44% of ophthalmoscopically invisible lesions could be detected and lesions that were under- or overexposed could be distinguished using the OCT data.

  2. Optical Demonstration of a Medical Imaging System with an EMCCD-Sensor Array for Use in a High Resolution Dynamic X-ray Imager.

    PubMed

    Qu, Bin; Huang, Ying; Wang, Weiyuan; Sharma, Prateek; Kuhls-Gilcrist, Andrew T; Cartwright, Alexander N; Titus, Albert H; Bednarek, Daniel R; Rudin, Stephen

    2010-10-30

    Use of an extensible array of Electron Multiplying CCDs (EMCCDs) in medical x-ray imager applications was demonstrated for the first time. The large variable electronic-gain (up to 2000) and small pixel size of EMCCDs provide effective suppression of readout noise compared to signal, as well as high resolution, enabling the development of an x-ray detector with far superior performance compared to conventional x-ray image intensifiers and flat panel detectors. We are developing arrays of EMCCDs to overcome their limited field of view (FOV). In this work we report on an array of two EMCCD sensors running simultaneously at a high frame rate and optically focused on a mammogram film showing calcified ducts. The work was conducted on an optical table with a pulsed LED bar used to provide a uniform diffuse light onto the film to simulate x-ray projection images. The system can be selected to run at up to 17.5 frames per second or even higher frame rate with binning. Integration time for the sensors can be adjusted from 1 ms to 1000 ms. Twelve-bit correlated double sampling AD converters were used to digitize the images, which were acquired by a National Instruments dual-channel Camera Link PC board in real time. A user-friendly interface was programmed using LabVIEW to save and display 2K × 1K pixel matrix digital images. The demonstration tiles a 2 × 1 array to acquire increased-FOV stationary images taken at different gains and fluoroscopic-like videos recorded by scanning the mammogram simultaneously with both sensors. The results show high resolution and high dynamic range images stitched together with minimal adjustments needed. The EMCCD array design allows for expansion to an M×N array for arbitrarily larger FOV, yet with high resolution and large dynamic range maintained.

  3. Ultra-High Resolution Optical Coherence Tomography Imaging of Unilateral Drusen in a 31 Year Old Woman

    PubMed Central

    de Carlo, Talisa E; Adhi, Mehreen; Lu, Chen D; Duker, Jay S; Fujimoto, James G; Waheed, Nadia K

    2016-01-01

    We report a case of widespread unilateral drusen in a healthy 31 year old Caucasian woman using multi-modal imaging including ultra-high resolution optical coherence tomography (UHR-OCT). Dilated fundus exam showed multiple drusen-like lesions in the posterior pole without heme or fluid. Fundus auto fluorescence demonstrated hyperautofluorescent at the deposits. Fluorescein angiography revealed mild hyperfluorescence and staining of the lesions. Spectral-domain optical coherence tomography (SD-OCT) OS showed accumulations in the temporal macula at Bruch’s membrane. UHR-OCT provided improved axial resolution compared to the standard 5 μm on the commercial SD-OCT and confirmed the presence of deposits in Bruch’s membrane, consistent with drusen. The retinal layers were draped over the excrescences but did not show any disruption. PMID:27398405

  4. High-resolution full-field optical coherence microscopy using a Mirau interferometer for the quantitative imaging of biological cells.

    PubMed

    Anna, Tulsi; Srivastava, Vishal; Mehta, Dalip Singh; Shakher, Chandra

    2011-12-01

    In this paper quantitative imaging of biological cells using high-resolution full-field optical coherence microscopy (FF-OCM) is reported. The FF-OCM was realized using a swept-source system, a Mirau interferometer, and a CCD camera (a two-dimensional detection unit). A Mirau-interferometric objective lens was used to generate the interferometric signal. The signal was analyzed by a Fourier analysis technique. Optically sectioned amplitude images and a quantitative phase map of biological cells such as onion skin and red blood cells (RBCs) are demonstrated. Further, the refractive index profile of the RBCs is also presented. For the 50× Mirau objective, the experimentally achieved axial and transverse resolution of the present system are 3.8 and 1.2 μm, respectively. The CCD provides parallel detection and measures enface images without X, Y, Z mechanical scanning.

  5. Investigation of solar active regions at high resolution by balloon flights of the solar optical universal polarimeter, definition phase

    NASA Technical Reports Server (NTRS)

    Tarbell, Theodore D.; Topka, Kenneth P.

    1992-01-01

    The definition phase of a scientific study of active regions on the sun by balloon flight of a former Spacelab instrument, the Solar Optical Universal Polarimeter (SOUP) is described. SOUP is an optical telescope with image stabilization, tunable filter and various cameras. After the flight phase of the program was cancelled due to budgetary problems, scientific and engineering studies relevant to future balloon experiments of this type were completed. High resolution observations of the sun were obtained using SOUP components at the Swedish Solar Observatory in the Canary Islands. These were analyzed and published in studies of solar magnetic fields and active regions. In addition, testing of low-voltage piezoelectric transducers was performed, which showed they were appropriate for use in image stabilization on a balloon.

  6. A scanning, all-fiber Sagnac interferometer for high resolution magneto-optic measurements at 820 nm

    SciTech Connect

    Fried, Alexander; Fejer, Martin; Kapitulnik, Aharon

    2014-10-15

    The Sagnac Interferometer has historically been used for detecting non-reciprocal phenomena, such as rotation. We demonstrate an apparatus in which this technique is employed for high resolution measurements of the Magneto-Optical Polar Kerr effect—a direct indicator of magnetism. Previous designs have incorporated free-space components which are bulky and difficult to align. We improve upon this technique by using all fiber-optic coupled components and demonstrate operation at a new wavelength, 820 nm, with which we can achieve better than 1 μrad resolution. Mounting the system on a piezo-electric scanner allows us to acquire diffraction limited images with 1.5 μm spatial resolution. We also provide extensive discussion on the details and of the Sagnac Interferometer's construction.

  7. High-Resolution Seismic Tomography from Dense Array Recordings of the August 23, 2011 Central Virginia Earthquake Aftershock Sequence

    NASA Astrophysics Data System (ADS)

    Davenport, K.; Hole, J. A.; Chapman, M. C.; Han, L.; Quiros, D. A.; Brown, L. D.; Mooney, W. D.

    2012-12-01

    The M 5.8 earthquake on August 23, 2011 in Louisa Co. Virginia is the largest event recorded in the Central Virginia Seismic Zone. This intraplate seismic zone is comprised of many poorly-defined seismogenic structures. Specific faults responsible for earthquakes in the CVSZ and other eastern US seismic zones are usually not identified due to relatively low strain rates and sparse seismic networks. The August 23, 2011 event provided an opportunity to test a novel type of high-density aftershock deployment that will define the associated fault structures at higher resolution than traditional aftershock networks. The AIDA (Aftershock Imaging with Dense Arrays) survey was designed to record wavefields at sufficiently dense spacing to minimize spatial aliasing and lower the event detection threshold. This use of array methods will allow the location of aftershocks with high precision and the imaging of geologic structure with resolution on par with typical controlled-source crustal surveys. Beginning August 27, AIDA deployed 201 stations in three phases, including lines with 200-m station spacing above the aftershock zone and a 60-km profile of three-component sensors to quantify regional attenuation characteristics. Preliminary work has focused on joint tomography for seismic velocity and hypocenter locations, vertical seismic profile event stacking, and ambient noise imaging. Preliminary aftershock locations define a best-fitting plane striking ~25° and dipping 55° E, consistent with the moment tensor solution for the main shock. Work in progress includes locating more of the >1000 events that have high signal-to-noise, event imaging using reverse-time waveform migration, and applying interferometry methods to image earth structure. The resulting high-resolution subsurface images and aftershock characterization will help constrain the geologic model of the region, and provide a template for future high-density aftershock deployments.

  8. Radiocarbon variability in the western equatorial Pacific inferred from a high-resolution coral record from Nauru Island

    SciTech Connect

    Guilderson, T.P.; Schrag, D.P.; Kashgarian, M.; Southon, J.

    1998-10-01

    We have generated a high resolution coral {Delta}{sup 14}C record spanning the last 50 years to document the seasonal and interannual redistribution of surface waters in the western tropical Pacific. Prebomb (1947{endash}1956) {Delta}{sup 14}C values average {minus}63{per_thousand} and have a total range of 30{per_thousand}. Values begin to increase in 1957, reaching a maximum of 137{per_thousand} in mid-1983. Large interannual variability of up to 80{per_thousand} closely follows the El Ni{tilde n}o-Southern Oscillation (ENSO). During each ENSO warm phase, {Delta}{sup 14}C values begin to increase, reflecting the reduction of low-{sup 14}C water upwelling in the east and the invasion of subtropical water into the western equatorial tropical Pacific. Maximum {Delta}{sup 14}C values are in phase or lag the corresponding sea surface temperature maxima in the eastern tropical Pacific, whereas the rapid return to more negative {Delta}{sup 14}C is in phase with eastern Pacific ENSO indices. The highest-amplitude excursions occur during the 1965/1966 and 1972/1973 events, when the {sup 14}C contrast is highest between the eastern Pacific and subtropics. The 1982/1983 El Ni{tilde n}o, although a larger ENSO event, has a lower {Delta}{sup 14}C amplitude, reflecting the penetration of bomb radiocarbon into the equatorial undercurrent and the reduced contrast in {Delta}{sup 14}C between thermocline and subtropical surface waters at that time. This coral record demonstrates the potential for using similar radiocarbon time series for documenting variability in Pacific shallow circulation over interannual and decadal timescales. {copyright} 1998 American Geophysical Union

  9. Fiber optic multipoint high-resolution level sensor for biomedical applications

    NASA Astrophysics Data System (ADS)

    Khotiaintsev, Sergei N.; de Leon Paredes, Victor; Molina-Flores, Esteban; Zemliak, A.; Svirid, V.; Matiuch, A.; Kosinsky, V.

    1995-01-01

    Fiber-optic sensors are attractive for biomedical applications primarily because of their non- electrical and non-conductive nature. This secures the safety of use and immunity to electromagnetic interference. In addition to these advantages, the optical-fiber level-sensor presented here has a small size, high accuracy, and fast time response. The sensor probe is chemically passive and can be easily sterilized. These features make the sensor attractive for numerous applications in physiology, biochemistry and medicine. The level-sensor presented here is a multipoint discrete optical-fiber device employing an array of small-size liquid- sensitive optical refractometric transducers of new type. The array of such transducers is connected via multi-fiber optical cable to the optoelectronic transmitter-receiver unit of the sensor. It performs an intelligent quasi-continuous level monitoring of a fluid. The sensor accuracy, resolution and level measurement range depend on the array length and the total number of the transducers employed. In case of the most close spacing of the transducers, the sensor level-resolution is better than plus or minus 0.2 mm.

  10. Three-Dimensional High-Resolution Optical/X-Ray Stereoscopic Tracking Velocimetry

    NASA Technical Reports Server (NTRS)

    Cha, Soyoung S.; Ramachandran, Narayanan

    2004-01-01

    Measurement of three-dimensional (3-D) three-component velocity fields is of great importance in a variety of research and industrial applications for understanding materials processing, fluid physics, and strain/displacement measurements. The 3-D experiments in these fields most likely inhibit the use of conventional techniques, which are based only on planar and optically-transparent-field observation. Here, we briefly review the current status of 3-D diagnostics for motion/velocity detection, for both optical and x-ray systems. As an initial step for providing 3-D capabilities, we nave developed stereoscopic tracking velocimetry (STV) to measure 3-D flow/deformation through optical observation. The STV is advantageous in system simplicity, for continually observing 3- D phenomena in near real-time. In an effort to enhance the data processing through automation and to avoid the confusion in tracking numerous markers or particles, artificial neural networks are employed to incorporate human intelligence. Our initial optical investigations have proven the STV to be a very viable candidate for reliably measuring 3-D flow motions. With previous activities are focused on improving the processing efficiency, overall accuracy, and automation based on the optical system, the current efforts is directed to the concurrent expansion to the x-ray system for broader experimental applications.

  11. Three-Dimensional High-Resolution Optical/X-Ray Stereoscopic Tracking Velocimetry

    NASA Technical Reports Server (NTRS)

    Cha, Soyoung S.; Ramachandran, Naryanan

    2005-01-01

    Measurement of three-dimensional (3-D) three-component velocity fields is of great importance in a variety of research and industrial applications for understanding materials processing, fluid physics, and strain/displacement measurements. The 3-D experiments in these fields most likely inhibit the use of conventional techniques, which are based only on planar and optically-transparent-field observation. Here, we briefly review the current status of 3-D diagnostics for motion/velocity detection, for both optical and x-ray systems. As an initial step for providing 3-D capabilities, we have developed stereoscopic tracking velocimetry (STV) to measure 3-D flow/deformation through optical observation. The STV is advantageous in system simplicity, for continually observing 3-D phenomena in near real-time. In an effort to enhance the data processing through automation and to avoid the confusion in tracking numerous markers or particles, artificial neural networks are employed to incorporate human intelligence. Our initial optical investigations have proven the STV to be a very viable candidate for reliably measuring 3-D flow motions. With previous activities focused on improving the processing efficiency, overall accuracy, and automation based on the optical system, the current efforts is directed to the concurrent expansion to the x-ray system for broader experimental applications.

  12. Three-Dimensional High-Resolution Optical/X-Ray Stereoscopic Tracking Velocimetry

    NASA Technical Reports Server (NTRS)

    Cha, Soyoung S.; Ramachandran, Narayanan

    2004-01-01

    Measurement of three-dimensional (3-D) three-component velocity fields is of great importance in a variety of research and industrial applications for understanding materials processing, fluid physics, and strain/displacement measurements. The 3-D experiments in these fields most likely inhibit the use of conventional techniques, which are based only on planar and optically-transparent-field observation. Here, we briefly review the current status of 3-D diagnostics for motion/velocity detection, for both optical and x-ray systems. As an initial step for providing 3-D capabilities, we nave developed stereoscopic tracking velocimetry (STV) to measure 3-D flow/deformation through optical observation. The STV is advantageous in system simplicity, for continually observing 3- D phenomena in near real-time. In an effort to enhance the data processing through automation and to avoid the confusion in tracking numerous markers or particles, artificial neural networks are employed to incorporate human intelligence. Our initial optical investigations have proven the STV to be a very viable candidate for reliably measuring 3-D flow motions. With previous activities are focused on improving the processing efficiency, overall accuracy, and automation based on the optical system, the current efforts is directed to the concurrent expansion to the x-ray system for broader experimental applications.

  13. An Optoelectronic Equivalent Narrowband Filter for High Resolution Optical Spectrum Analysis.

    PubMed

    Feng, Kunpeng; Cui, Jiwen; Dang, Hong; Wu, Weidong; Sun, Xun; Jiang, Xuelin; Tan, Jiubin

    2017-02-10

    To achieve a narrow bandwidth optical filter with a wide swept range for new generation optical spectrum analysis (OSA) of high performance optical sensors, an optoelectronic equivalent narrowband filter (OENF) was investigated and a swept optical filter with bandwidth of several MHz and sweep range of several tens of nanometers was built using electric filters and a sweep laser as local oscillator (LO). The principle of OENF is introduced and analysis of the OENF system is presented. Two electric filters are optimized to be RBW filters for high and medium spectral resolution applications. Both simulations and experiments are conducted to verify the OENF principle and the results show that the power uncertainty is less than 1.2% and the spectral resolution can reach 6 MHz. Then, a real-time wavelength calibration system consisting of a HCN gas cell and Fabry-Pérot etalon is proposed to guarantee a wavelength accuracy of ±0.4 pm in the C-band and to reduce the influence of phase noise and nonlinear velocity of the LO sweep. Finally, OSA experiments on actual spectra of various optical sensors are conducted using the OENF system. These experimental results indicate that OENF system has an excellent capacity for the analysis of fine spectrum structures.

  14. An Optoelectronic Equivalent Narrowband Filter for High Resolution Optical Spectrum Analysis

    PubMed Central

    Feng, Kunpeng; Cui, Jiwen; Dang, Hong; Wu, Weidong; Sun, Xun; Jiang, Xuelin; Tan, Jiubin

    2017-01-01

    To achieve a narrow bandwidth optical filter with a wide swept range for new generation optical spectrum analysis (OSA) of high performance optical sensors, an optoelectronic equivalent narrowband filter (OENF) was investigated and a swept optical filter with bandwidth of several MHz and sweep range of several tens of nanometers was built using electric filters and a sweep laser as local oscillator (LO). The principle of OENF is introduced and analysis of the OENF system is presented. Two electric filters are optimized to be RBW filters for high and medium spectral resolution applications. Both simulations and experiments are conducted to verify the OENF principle and the results show that the power uncertainty is less than 1.2% and the spectral resolution can reach 6 MHz. Then, a real-time wavelength calibration system consisting of a HCN gas cell and Fabry–Pérot etalon is proposed to guarantee a wavelength accuracy of ±0.4 pm in the C-band and to reduce the influence of phase noise and nonlinear velocity of the LO sweep. Finally, OSA experiments on actual spectra of various optical sensors are conducted using the OENF system. These experimental results indicate that OENF system has an excellent capacity for the analysis of fine spectrum structures. PMID:28208624

  15. River pollution remediation monitored by optical and infrared high-resolution satellite images.

    PubMed

    Trivero, Paolo; Borasi, Maria; Biamino, Walter; Cavagnero, Marco; Rinaudo, Caterina; Bonansea, Matias; Lanfri, Sofia

    2013-09-01

    The Bormida River Basin, located in the northwestern region of Italy, has been strongly contaminated by the ACNA chemical factory. This factory was in operation from 1892 to 1998, and contamination from the factory has had deleterious consequences on the water quality, agriculture, natural ecosystems and human health. Attempts have been made to remediate the site. The aims of this study were to use high-resolution satellite images combined with a classical remote sensing methodology to monitor vegetation conditions along the Bormida River, both upstream and downstream of the ACNA chemical factory site, and to compare the results obtained at different times before and after the remediation process. The trends of the Normalised Difference Vegetation Index (NDVI) and the Enhanced Vegetation Index (EVI) along the riverbanks are used to assess the effect of water pollution on vegetation. NDVI and EVI values show that the contamination produced by the ACNA factory had less severe effects in the year 2007, when most of the remediation activities were concluded, than in 2006 and 2003. In 2007, the contamination effects were noticeable up to 6 km downstream of the factory, whereas in 2003 and 2006 the influence range was up to about 12 km downstream of the factory. The results of this study show the effectiveness of remediation activities that have been taking place in this area. In addition, the comparison between NDVI and EVI shows that the EVI is more suitable to characterise the vegetation health and can be considered an additional tool to assess vegetation health and to monitor restoration activities.

  16. Extended-focus optical coherence microscopy for high-resolution imaging of the murine brain

    PubMed Central

    Tamborski, Szymon; Lyu, Hong Chou; Dolezyczek, Hubert; Malinowska, Monika; Wilczynski, Grzegorz; Szlag, Daniel; Lasser, Theo; Wojtkowski, Maciej; Szkulmowski, Maciej

    2016-01-01

    We propose a new method and optical instrumentation for mouse brain imaging based on extended-focus optical coherence microscopy. This in vivo imaging technique allows the evaluation of the cytoarchitecture at cellular level and the circulation system dynamics in three dimensions. This minimally invasive and non-contact approach is performed without the application of contrasting agents. The optical design achieved a resolution of 2.2 μm over a distance of 800 μm, which was sufficient to obtain a detailed three-dimensional image of a wild-type mouse’s brain down to the layer III of the cortex. Intrinsically contrasted microvessels and structures similar to the bodies of neurons were distinguishable. PMID:27895982

  17. Broadband miniature optical ultrasound probe for high resolution vascular tissue imaging

    PubMed Central

    Colchester, Richard J.; Zhang, Edward Z.; Mosse, Charles A.; Beard, Paul C.; Papakonstantinou, Ioannis; Desjardins, Adrien E.

    2015-01-01

    An all-optical ultrasound probe for vascular tissue imaging was developed. Ultrasound was generated by pulsed laser illumination of a functionalized carbon nanotube composite coating on the end face of an optical fiber. Ultrasound was detected with a Fabry-Pérot (FP) cavity on the end face of an adjacent optical fiber. The probe diameter was < 0.84 mm and had an ultrasound bandwidth of ~20 MHz. The probe was translated across the tissue sample to create a virtual linear array of ultrasound transmit/receive elements. At a depth of 3.5 mm, the axial resolution was 64 µm and the lateral resolution was 88 µm, as measured with a carbon fiber target. Vascular tissues from swine were imaged ex vivo and good correspondence to histology was observed. PMID:25909031

  18. High-resolution imaging of neoplastic lesions using optical coherence tomography

    NASA Astrophysics Data System (ADS)

    Pitris, Constantinos; Goodman, Annekathryn; Boppart, Stephen A.; Drexler, Wolfgang; Jesser, Christine; Stamper, Debra L.; Brezinski, Mark E.; Fujimoto, James G.

    1999-04-01

    A technology capable of imaging tissue, at or near the cellular level, could lead to the detection of neoplasias at earlier stages than currently possible. This could significantly improve patient outcomes, since once cancer becomes metastatic, cure is difficult. Optical coherence tomography (OCT), a recently developed imaging technology, has ben shown to achieve resolution in the cellular and subcellular range, and it could improve the diagnostic range of clinical imaging procedures. To assess the clinical applicability of OCT, neoplastic specimens from the urinary, gastrointestinal and female reproductive tract were imaged. Sharp differentiation of structures included the mucosa/submucosal/muscularis boundaries, epithelium, glands, supportive tissue, and intramural cysts. The ability of optical coherence tomography to image tissue microstructure at or near the cellular level make it a potentially powerful technology for minimally invasive assessment of tissue microstructure. The resolution of optical coherence tomography, which is greater than any current clinical imaging modality, make it particularly attractive for the assessment of early neoplastic changes.

  19. High-Resolution Optical and Near-Infrared Imaging of Young Circumstellar Disks

    NASA Technical Reports Server (NTRS)

    McCaughrean, Mark; Stapelfeldt, Karl; Close, Laird

    2000-01-01

    In the past five years, observations at optical and near-infrared wavelengths obtained with the Hubble Space Telescope and ground-based adaptive optics have provided the first well-resolved images of young circumstellar disks which may form planetary systems. We review these two observational techniques and highlight their results by presenting prototype examples of disks imaged in the Taurus-Auriga and Orion star-forming regions. As appropriate, we discuss the disk parameters that may be typically derived from the observations, as well as the implications that the observations may have on our understanding of, for example, the role of the ambient environment in shaping the disk evolution. We end with a brief summary of the prospects for future improvements in space- and ground-based optical/IR imaging techniques, and how they may impact disk studies.

  20. High-resolution optical frequency dissemination on a telecommunications network with data traffic.

    PubMed

    Kéfélian, Fabien; Lopez, Olivier; Jiang, Haifeng; Chardonnet, Christian; Amy-Klein, Anne; Santarelli, Giorgio

    2009-05-15

    We transferred the frequency of an ultrastable laser over a 108-km-long urban fiber link comprising 22 km of an optical communications network fiber simultaneously carrying Internet data traffic. The metrological signal and the digital data signal were transferred over two different frequency channels in a dense wavelength-division multiplexing scheme. The metrological signal was inserted in and extracted from the communication network using bidirectional off-the-shelf optical add-drop multiplexers. The link-induced phase noise was measured and canceled with a round-trip technique using an all-fiber-based interferometer. The compensated link showed an Allan deviation of a few 10(-16) at 1 s and below 10(-19) at 10,000 s. This work paves the way to a wide dissemination of ultrastable optical clock signals between distant laboratories via the Internet.

  1. Characterization of Biogeochemical Variability in a Tidal Estuary Using High Resolution Optical Measurements

    NASA Astrophysics Data System (ADS)

    Chang, G.; Jones, C.; Martin, T.

    2015-12-01

    The Berry's Creek Study Area (BCSA) is a tidal estuary located in New Jersey. Several chemicals of potential concern (COPCs) are present in the BCSA waterway and marshes, including mercury, methyl mercury, and polychlorinated biphenyls. Concentrations of COPCs and suspended solids in the BCSA vary temporally and spatially due to tidal variability, freshwater flow events, and interaction of marsh, waterway, and sediment bed materials. This system-wide variability confounds evaluation of COPC sources and transport mechanisms when using conventional laboratory-based analysis of discrete water column samples. Therefore, an optically-based biogeochemical monitoring program was conducted using near-continuous measurements of optical properties and an optical-biogeochemical partial least-squares regression model pioneered by B. Bergamaschi (USGS) and colleagues. The objective of the study was to characterize COPC concentration dynamics in the BCSA water column and relate the analysis to sediment bed processes. Optical-biogeochemical model results indicated that, in general, measured optical properties were sufficient for predicting COPC concentrations to within 10% of the accuracy of laboratory-based analytical measurements. The continuous, high temporal resolution time series of COPC concentrations determined by the optical-biogeochemical model enabled evaluation of the sediment bed dynamics and variability of COPCs in the surface water of the BCSA. Results indicate that tidally-induced resuspension of waterway sediment bed particulates is the primary mechanism for transport of COPCs to surface water. Waterway-marsh tidal exchange shows a net mass flux of particulate COPCs from waterway to marsh, indicating that particulate COPCs are retained and accumulate in the marshes with relatively little net export of dissolved COPCs from the marshes to the waterway.

  2. A high-resolution temporal record of environmental changes in the Eastern Caribbean (Guadeloupe) from 40 to 10 ka BP

    NASA Astrophysics Data System (ADS)

    Royer, Aurélien; Malaizé, Bruno; Lécuyer, Christophe; Queffelec, Alain; Charlier, Karine; Caley, Thibaut; Lenoble, Arnaud

    2017-01-01

    In neotropical regions, fossil bat guano accumulated over time as laminated layers in caves, hence providing a high-resolution temporal record of terrestrial environmental changes. Additionally, cave settings have the property to preserve such organic sediments from processes triggered by winds (deflation, abrasion and sandblasting) and intense rainfall (leaching away). This study reports both stable carbon and nitrogen isotope compositions of frugivorous bat guano deposited in a well-preserved stratigraphic succession of Blanchard Cave on Marie-Galante, Guadeloupe. These isotopic data are discussed with regard to climate changes and its specific impact on Eastern Caribbean vegetation during the Late Pleistocene from 40 to 10 ka cal. BP. Guano δ13C values are higher than modern ones, suggesting noticeable vegetation changes. This provides also evidence for overall drier environmental conditions during the Pleistocene compared to today. Meanwhile, within this generally drier climate, shifts between wetter and drier conditions can be observed. Large temporal amplitudes in both δ13C and δ15N variations reaching up to 5.9‰ and 16.8‰, respectively, also indicate these oceanic tropical environments have been highly sensitive to regional or global climatic forcing. Stable isotope compositions of bat guano deposited from 40 to 35 ka BP, the Last Glacial Maximum and the Younger-Dryas reveal relatively wet environmental conditions whereas, at least from the end of the Heinrich event 1 and the Bølling period the region experienced drier environmental conditions. Nevertheless, when considering uncertainties in the model age, the isotopic record of Blanchard Cave show relatively similar variations with known proxy records from the northern South America and Central America, suggesting thus that the Blanchard Cave record is a robust proxy of past ITCZ migration. Teleconnections through global atmospheric pattern suggest that islands of the eastern Caribbean Basin could

  3. High-Resolution Two-Dimensional Optical Spectroscopy of Electron Spins

    NASA Astrophysics Data System (ADS)

    Salewski, M.; Poltavtsev, S. V.; Yugova, I. A.; Karczewski, G.; Wiater, M.; Wojtowicz, T.; Yakovlev, D. R.; Akimov, I. A.; Meier, T.; Bayer, M.

    2017-07-01

    Multidimensional coherent optical spectroscopy is one of the most powerful tools for investigating complex quantum mechanical systems. While it was conceived decades ago in magnetic resonance spectroscopy using microwaves and radio waves, it has recently been extended into the visible and UV spectral range. However, resolving MHz energy splittings with ultrashort laser pulses still remains a challenge. Here, we analyze two-dimensional Fourier spectra for resonant optical excitation of resident electrons to localized trions or donor-bound excitons in semiconductor nanostructures subject to a transverse magnetic field. Particular attention is devoted to Raman coherence spectra, which allow one to accurately evaluate tiny splittings of the electron ground state and to determine the relaxation times in the electron spin ensemble. A stimulated steplike Raman process induced by a sequence of two laser pulses creates a coherent superposition of the ground-state doublet which can be retrieved only optically because of selective excitation of the same subensemble with a third pulse. This provides the unique opportunity to distinguish between different complexes that are closely spaced in energy in an ensemble. The related experimental demonstration is based on photon-echo measurements in an n -type CdTe /(Cd ,Mg )Te quantum-well structure detected by a heterodyne technique. The difference in the sub-μ eV range between the Zeeman splittings of donor-bound electrons and electrons localized at potential fluctuations can be resolved even though the homogeneous linewidth of the optical transitions is larger by 2 orders of magnitude.

  4. High Resolution Optical/Near-Infrared Imaging of Cool Ultraluminous Infrared Galaxies

    NASA Technical Reports Server (NTRS)

    Surace, J.; Sanders, D.; Evans, A.

    1999-01-01

    We present here new multiwavelength observations with 1.5 and 4x the spatial resolution of previous ground-based observations at optical and near-infrared wavelengths; despite being ground-based, they allow us to isolate interesting features such as the star-forming knots detected in the warm ULIG sample.

  5. High-Resolution Radial Velocity Mapping of Optical Filaments in Evolved Supernova Remnants

    NASA Astrophysics Data System (ADS)

    Greidanus, H.; Strom, R. G.

    The authors report on observations of the kinematical structure of optical filaments in evolved supernova remnants, using an imaging Fabry-Perot interferometer. The radial velocity characteristics as seen in [O III] λ5007 emission in one area in the Cygnus Loop are described, where four kinematically different components contributing to the emission can be recognized.

  6. High Resolution Optical/Near-Infrared Imaging of Cool Ultraluminous Infrared Galaxies

    NASA Technical Reports Server (NTRS)

    Surace, J.; Sanders, D.; Evans, A.

    1999-01-01

    We present here new multiwavelength observations with 1.5 and 4x the spatial resolution of previous ground-based observations at optical and near-infrared wavelengths; despite being ground-based, they allow us to isolate interesting features such as the star-forming knots detected in the warm ULIG sample.

  7. Interrogating biology with force: single molecule high-resolution measurements with optical tweezers.

    PubMed

    Capitanio, Marco; Pavone, Francesco S

    2013-09-17

    Single molecule force spectroscopy methods, such as optical and magnetic tweezers and atomic force microscopy, have opened up the possibility to study biological processes regulated by force, dynamics of structural conformations of proteins and nucleic acids, and load-dependent kinetics of molecular interactions. Among the various tools available today, optical tweezers have recently seen great progress in terms of spatial resolution, which now allows the measurement of atomic-scale conformational changes, and temporal resolution, which has reached the limit of the microsecond-scale relaxation times of biological molecules bound to a force probe. Here, we review different strategies and experimental configurations recently developed to apply and measure force using optical tweezers. We present the latest progress that has pushed optical tweezers' spatial and temporal resolution down to today's values, discussing the experimental variables and constraints that are influencing measurement resolution and how these can be optimized depending on the biological molecule under study. Copyright © 2013 Biophysical Society. Published by Elsevier Inc. All rights reserved.

  8. Fiber-Optic Strain Gauge With High Resolution And Update Rate

    NASA Technical Reports Server (NTRS)

    Figueroa, Fernando; Mahajan, Ajay; Sayeh, Mohammad; Regez, Bradley

    2007-01-01

    An improved fiber-optic strain gauge is capable of measuring strains in the approximate range of 0 to 50 microstrains with a resolution of 0.1 microstrain. (To some extent, the resolution of the strain gauge can be tailored and may be extensible to 0.01 microstrain.) The total cost of the hardware components of this strain gauge is less than $100 at 2006 prices. In comparison with prior strain gauges capable of measurement of such low strains, this strain gauge is more accurate, more economical, and more robust, and it operates at a higher update rate. Strain gauges like this one are useful mainly for measuring small strains (including those associated with vibrations) in such structures as rocket test stands, buildings, oilrigs, bridges, and dams. The technology was inspired by the need to measure very small strains on structures supporting liquid oxygen tanks, as a way to measure accurately mass of liquid oxygen during rocket engine testing. This improved fiber-optic strain gauge was developed to overcome some of the deficiencies of both traditional foil strain gauges and prior fiber-optic strain gauges. Traditional foil strain gages do not have adequate signal-to-noise ratios at such small strains. Fiber-optic strain gauges have been shown to be potentially useful for measuring such small strains, but heretofore, the use of fiberoptic strain gauges has been inhibited, variously, by complexity, cost, or low update rate.

  9. Individual sarcomere length determination from isolated cardiac cells using high-resolution optical microscopy and digital image processing.

    PubMed Central

    Roos, K P; Brady, A J

    1982-01-01

    Discrete sarcomere lengths have been determined from dynamically contracting isolated cardiac cells with a high-speed, high-resolution direct optical imaging system. Calcium-tolerant cardiac cells from the rat are isolated by perfusion with collagenase and hyaluronidase. Individual sarcomere lengths can be determined by directly imaging the cell's striation pattern onto a solid-state charge-coupled device (CCD) detector interfaced with a digital computer. The precision of detection in a real light microscopic optical system is discussed in relation to the type of image detector, optical contract enhancement techniques, and digital image processing. The optical performance of the direct striation pattern image apparatus has been determined empirically with test grids under standard bright-field and Nomarski-differential interference contrast (DIC) conditions for application to real muscle imaging. Discrete striation positions of isolated cells have been detected and followed with high precision during phasic contraction-relaxation cycles down to average sarcomere lengths as short as 1.43 +/- 0.053 microns. The maximum rates of contraction and relaxation are rapid and synchronous in time course along the length of the cell. These results indicate that direct optical imaging can provide an accurate means to monitor discrete striations and sarcomere lengths along the length of Ca2+-tolerant heart cells. Images FIGURE 1 FIGURE 4 PMID:7183337

  10. High-resolution image reconstruction technique applied to the optical testing of ground-based astronomical telescopes

    NASA Astrophysics Data System (ADS)

    Jin, Zhenyu; Lin, Jing; Liu, Zhong

    2008-07-01

    By study of the classical testing techniques (such as Shack-Hartmann Wave-front Sensor) adopted in testing the aberration of ground-based astronomical optical telescopes, we bring forward two testing methods on the foundation of high-resolution image reconstruction technology. One is based on the averaged short-exposure OTF and the other is based on the Speckle Interferometric OTF by Antoine Labeyrie. Researches made by J.Ohtsubo, F. Roddier, Richard Barakat and J.-Y. ZHANG indicated that the SITF statistical results would be affected by the telescope optical aberrations, which means the SITF statistical results is a function of optical system aberration and the atmospheric Fried parameter (seeing). Telescope diffraction-limited information can be got through two statistics methods of abundant speckle images: by the first method, we can extract the low frequency information such as the full width at half maximum (FWHM) of the telescope PSF to estimate the optical quality; by the second method, we can get a more precise description of the telescope PSF with high frequency information. We will apply the two testing methods to the 2.4m optical telescope of the GMG Observatory, in china to validate their repeatability and correctness and compare the testing results with that of the Shack-Hartmann Wave-Front Sensor got. This part will be described in detail in our paper.

  11. Light-directed functionalization methods for high-resolution optical fiber based biosensors

    NASA Astrophysics Data System (ADS)

    Kahyaoglu, Leyla Nesrin; Madangopal, Rajtarun; Stensberg, Matthew; Rickus, Jenna L.

    2005-05-01

    Recent advances in miniaturization and analyte-sensitive fluorescent indicators make optical fiber biosensors promising alternatives to microelectrodes. Optical sensing offers several advantages over electrochemical methods including increased stability and better spatial control to monitor physiological processes at cellular resolutions. The distal end of an optical fiber can be functionalized with different fluorophore/polymer combinations through mechanical, dip-coating or photopolymerization techniques. Unlike mechanical and dip-coating schemes, photopolymerization can spatially confine the sensing layer in the vicinity of light in a more reproducible and controllable manner. The objective of this study was to fabricate microscale fluorescence lifetime based optrodes using UV-induced photopolymerization. Six commercially available acrylate based monomers were investigated for stable entrapment of the oxygen sensitive porphyrin dye (PtTFPP) dye via photopolymerization at the end of optical fibers. Of these, the acrylate-functionalized alkoxysilane monomer, 3-methacryloxypropyl-trimethoxysilane (tradename Dynasylan MEMO) showed maximal response to changes in oxygen concentration. Dye-doped polymer microtips were grown at the ends 50 μm optical fibers and sensitivity and response time were optimized by varying both the concentration of doped dye and the excitation power used for polymerization. The resulting sensors showed linear response within the physiologically relevant range of oxygen concentrations and fast response times. While applied here to oxygen sensing, the photopolymer formulation and process parameters described are compatible with a wide range of available organic dyes and can be used to pattern arrays of spots, needles or more complex shapes at high spatial resolution.

  12. Integration of Permanent Scatterers Analysis and High Resolution Optical Images within Landslide Risk Analysis

    NASA Astrophysics Data System (ADS)

    Farina, P.; Colombo, D.; Fumagalli, A.; Gontier, E.; Moretti, S.

    2004-06-01

    In the framework of the on-going SLAM project (Service for Landslide Monitoring), funded by the European Space Agency (ESA) the Permanent Scatterers processing and the analysis of high resolution images (e.g. SPOT5) have been performed at a basin scale, on the whole territory of the Arno River basin (Central Italy). The project aims at the development of a service based on the integration of EO-data within the current practices used for landslide risk management. The studied area, with a spatial extension of about 9,000 km2, has been chosen for the presence of a high number of mass movements (up today about 20,000 landslides have been mapped and 300 of them have been classified as at high risk from the institutional authorities) and for its significance, in terms of landslide typology and environmental conditions, with respect to the Italian Apennine territory. By considering the technical requirements imposed by the Italian legislation for the documents related to landslide risk management, three products based on EO- data have been defined related to landslide inventory mapping, landslide hazard mapping and landslide monitoring. To this aim, about 350 SAR images have been interferometrically processed by means of the PS technique, detecting about 600,000 PS. The processing of SPOT5 images and aerial-photos, still in progress, have been performed for the extraction of features related to the landslide presence, useful for the geomorphological analysis and, as a consequence in order to give a spatial meaning to the punctual information provided by the PS. This procedure has been coupled with a intense geological interpretation phase characterized by the analysis of traditional in situ monitoring data, ancillary data and the performing of field surveys. The results of the above described methodologies will impact on the current documents used by the Arno Basin Authority for the landslide risk management (e.g. P.A.I., Piano di Assetto Idrogeologico) creating a case

  13. Fibre-optic coupling to high-resolution CCD and CMOS image sensors

    NASA Astrophysics Data System (ADS)

    van Silfhout, R. G.; Kachatkou, A. S.

    2008-12-01

    We describe a simple method of gluing fibre-optic faceplates to complementary metal oxide semiconductor (CMOS) active pixel and charge coupled device (CCD) image sensors and report on their performance. Cross-sectional cuts reveal that the bonding layer has a thickness close to the diameter of the individual fibres and is uniform over the whole sensor area. Our method requires no special tools or alignment equipment and gives reproducible and high-quality results. The method maintains a uniform bond layer thickness even if sensor dies are mounted at slight angles with their package. These fibre-coupled sensors are of particular interest to X-ray imaging applications but also provide a solution for compact optical imaging systems.

  14. High-resolution optical spectroscopy using multimode interference in a compact tapered fibre.

    PubMed

    Wan, Noel H; Meng, Fan; Schröder, Tim; Shiue, Ren-Jye; Chen, Edward H; Englund, Dirk

    2015-07-23

    Optical spectroscopy is a fundamental tool in numerous areas of science and technology. Much effort has focused on miniaturizing spectrometers, but thus far at the cost of spectral resolution and broad operating range. Here we describe a compact spectrometer that achieves both high spectral resolution and broad bandwidth. The device relies on imaging multimode interference from leaky modes along a multimode tapered optical fibre, resulting in spectrally distinguishable spatial patterns over a wide range of wavelengths from 500 to 1,600 nm. This tapered fibre multimode interference spectrometer achieves a spectral resolution down to 40 pm in the visible spectrum and 10 pm in the near-infrared spectrum (corresponding to resolving powers of 10(4)-10(5)). Multimode interference spectroscopy is suitable in a variety of device geometries, including planar waveguides in a broad range of transparent materials.

  15. High-resolution mapping of quantum efficiency of silicon photodiode via optical-feedback laser microthermography

    SciTech Connect

    Cemine, Vernon Julius; Blanca, Carlo Mar; Saloma, Caesar

    2006-09-20

    We map the external quantum efficiency (QE) distribution of a silicon photodiode (PD) sample via a thermographic imaging technique based on optical-feedback laser confocal microscopy. An image pair consisting of the confocal reflectance image and the 2D photocurrent map is simultaneously acquired to delineate the following regions of interest on the sample: the substrate, the n-type region, the pn overlay, and the bonding pad. The 2D QE distribution is derived from the photocurrent map to quantify the optical performance of these sites. The thermal integrity of the sample is then evaluated by deriving the rate of change of QE with temperature T at each point on the silicon PD. These gradient maps function not only as stringent measures of local thermal QE activity but they also expose probable defect locations on the sample at high spatial resolution - a capability that is not feasible with existing bulk measurement techniques.

  16. Revealing past environmental changes on the Antarctic Peninsula by analyzing high resolution sedimentary records from Lake Esmeralda, Vega Island

    NASA Astrophysics Data System (ADS)

    Píšková, Anna; Nývlt, Daniel; Roman, Matěj; Lirio, Juan Manuel; Kopalová, Kateřina

    2017-04-01

    Topographically and climatically, the environment of the Antarctic Peninsula (AP) differs significantly from Continental Antarctica. The AP forms an unbroken chain of rugged, alpine topography, which forms a climatic barrier separating the warmer Bellingshausen Sea on the western coast from the colder Weddell Sea on the east. The AP has experienced one of the highest temperature increases on Earth in the second half of the 20th century as a response to the ongoing global warming (Turner et al., 2005). However, the last decade was colder and a significant decrease in air temperature was detected especially in the north-eastern part of the AP (Turner et al., 2016; Oliva et al., 2017). The extreme sensitivity of the area to climate change represents exceptional potential for AP palaeoclimatic records either from marine, lacustrine, or ice cores. We have analysed several sedimentary cores from yet unstudied Lake Esmeralda, which was formed as a result of the last deglaciation during the Pleistocene/Holocene transition. We here focus on the longest (177 cm) core that could record environmental changes of the past millennia. In order to describe both inorganic and organic components of the sediment we used a combination of the following methods: XRF, XRD, magnetic susceptibility measurement, chemical analysis for determination of cation exchange capacity, grain size analysis, geochemical analysis (TIC, TOC, TS), high pressure liquid chromatography (HPLC) and diatom community analysis. This combination of methods gave us an insight in the past environmental changes in the lake catchment as well as in the lake body. More specifically it enabled us to define periods of enhanced weathering and to distinguish colder and warmer phases. The preliminary results will be completed with age-depth model resulting in a high resolution multi-proxy record that will contribute to a better, more detailed picture of the past climatic and environmental changes in the north-eastern AP

  17. A Novel, High-Resolution, High-Speed Fiber-Optic Temperature Sensor for Oceanographic Applications

    DTIC Science & Technology

    2015-05-11

    sharp thermo -gradient underwater. Keywords— Fiber-optic thermometer; Fabry-Pérot interferometer; ocean microstructure; turbulence I...in this paper is based on a FP cavity formed by thin crystalline silicon film attached to the end face of a single-mode fiber. Due to the thermo ...the dynamic temperature variations associated with a strong microstructure thermo -gradient is demonstrated. A. Sensitivity (a) (b) Fig. 1. (a

  18. High-resolution absolute frequency referenced fiber optic sensor for quasi-static strain sensing

    SciTech Connect

    Lam, Timothy T.-Y.; Chow, Jong H.; Shaddock, Daniel A.; Littler, Ian C. M.; Gagliardi, Gianluca; Gray, Malcolm B.; McClelland, David E.

    2010-07-20

    We present a quasi-static fiber optic strain sensing system capable of resolving signals below nanostrain from 20 mHz. A telecom-grade distributed feedback CW diode laser is locked to a fiber Fabry-Perot sensor, transferring the detected signals onto the laser. An H{sup 13}C{sup 14}N absorption line is then used as a frequency reference to extract accurate low-frequency strain signals from the locked system.

  19. Recent developments in high-resolution optical diagnostics of repetitively pulsed laser-target effects

    NASA Astrophysics Data System (ADS)

    Hugenschmidt, Manfred; Althaus, Marion

    1995-05-01

    High energy densities, as required both in research and in industry, are achieved by the use of lasers. Extremely highpower densities are obtained in the pulsed mode with short microsecond(s) -, ns-, or even ultrashort ps- to fs- pulses. The interaction of such powerful laser pulses with any type of solid state, liquid or gaseous materials is then causing rapidly developing, nonstationary, optically nonlinear processes. Experimental investigations of these effects are therefore requiring special measuring techniques with high spatial and temporal resolution. Optical and optronical methods have proven to be particularly useful. Methods based on laser diagnostics, including high speed photography, cinematography, speckle techniques, holography, videography, infrared techniques or arbitrary combinations of these, are therefore considered to be important tools in these laser effect studies. The investigations reported in the present paper are referring to carbon dioxide-laser effects in intensity ranges which are useful for many industrial applications, such as for example in the field of material processing. Basic interest is actually in pulsed, plasma sustained laser target interaction phenomena which occur above critical threshold power densities, specific for each type of material. Surface induced, highly ionized absorption waves are then determining the energy transfer from the coherent laser radiation field towards the targets. The experiments at ISL were aimed at investigating plasma parameters and their influence on the energy transfer rates, by fast optical, electrical and optronical techniques, such as mentioned above. The results to be discussed refer to target effects, basically observed on optically transparent materials, subject to high average power pulsed carbon dioxide-laser radiation, with repetition rates of several tens to hundred pps at multi-MW/cm2 to GW/cm2 peak power densities and average power densities in the multi-kW/cm2-range.

  20. Ultra-high, resolution, modular optical angle encoder for space-based opto-mechanical applications

    NASA Technical Reports Server (NTRS)

    Luther, Holger; Beard, Paul; Mitchell, Donald; Thorburn, William

    1995-01-01

    A 27-bit optical encoder using a novel patent pending technology has been developed by the MicroE Development Center of BEI Sensors & Systems Company and tested by the Sensor Systems Group (SSG) Inc., in a positioning and stabilization mirror assembly (PSMA) designed and constructed under a grant from the Marshall Space Flight Center. Test results verified performance within the specifications of the PSMA.

  1. Five Micron High Resolution MALDI Mass Spectrometry Imaging with Simple, Interchangeable, Multi-Resolution Optical System

    NASA Astrophysics Data System (ADS)

    Feenstra, Adam D.; Dueñas, Maria Emilia; Lee, Young Jin

    2017-01-01

    High-spatial resolution mass spectrometry imaging (MSI) is crucial for the mapping of chemical distributions at the cellular and subcellular level. In this work, we improved our previous laser optical system for matrix-assisted laser desorption ionization (MALDI)-MSI, from 9 μm practical laser spot size to a practical laser spot size of 4 μm, thereby allowing for 5 μm resolution imaging without oversampling. This is accomplished through a combination of spatial filtering, beam expansion, and reduction of the final focal length. Most importantly, the new laser optics system allows for simple modification of the spot size solely through the interchanging of the beam expander component. Using 10×, 5×, and no beam expander, we could routinely change between 4, 7, and 45 μm laser spot size, in less than 5 min. We applied this multi-resolution MALDI-MSI system to a single maize root tissue section with three different spatial resolutions of 5, 10, and 50 μm and compared the differences in imaging quality and signal sensitivity. We also demonstrated the difference in depth of focus between the optical systems with 10× and 5× beam expanders.

  2. Development of compact and ultra-high-resolution spectrograph with multi-GHz optical frequency comb

    NASA Astrophysics Data System (ADS)

    Endo, Mamoru; Sukegawa, Takashi; Silva, Alissa; Kobayashi, Yohei

    2014-08-01

    In recent years, a calibration method for an astronomical spectrograph using an optical frequency comb (OFC) with a repetition rate of more than ten GHz has been developed successfully [1-5]. But controlling filtering cavities that are used for thinning out longitudinal modes precludes long term stability. The super-mode noise coming from the fundamental repetition rate is an additional problem. We developed a laser-diode pumped Yb:Y2O3 ceramic oscillator, which enabled the generation of 4-GHz (maximum repetition rate of 6.7 GHz) pulse trains directly with a spectrum width of 7 nm (full-width half-maximum, FWHM), and controlled its optical frequency within a MHz level of accuracy using a beat note between the 4-GHz laser and a 246-MHz Yb-fiber OFC. The optical frequency of the Yb-fiber OFC was phase locked to a Rb clock frequency standard. Furthermore we also built a table-top multi-pass spectrograph with a maximum frequency resolution of 600 MHz and a bandwidth of 1 nm using a large-size high-efficiency transmission grating. The resolution could be changed by selecting the number of passes through the grating. This spectrograph could resolve each longitudinal mode of our 4-GHz OFC clearly, and more than 10% throughput was obtained when the resolution was set to 600 MHz. We believe that small and middle scale astronomical observatories could easily implement such an OFC-calibrated spectrograph.

  3. X-ray microbeam measurements with a high resolution scintillator fibre-optic dosimeter.

    PubMed

    Archer, James; Li, Enbang; Petasecca, Marco; Dipuglia, Andrew; Cameron, Matthew; Stevenson, Andrew; Hall, Chris; Hausermann, Daniel; Rosenfeld, Anatoly; Lerch, Michael

    2017-09-29

    Synchrotron microbeam radiation therapy is a novel external beam therapy under investigation, that uses highly brilliant synchrotron x-rays in microbeams 50 μm width, with separation of 400 μm, as implemented here. Due to the fine spatial fractionation dosimetry of these beams is a challenging and complicated problem. In this proof-of-concept work, we present a fibre optic dosimeter that uses plastic scintillator as the radiation conversion material. We claim an ideal one-dimensional resolution of 50 μm. Using plastic scintillator and fibre optic makes this dosimeter water-equivalent, a very desirable dosimetric property. The dosimeter was tested at the Australian Synchrotron, on the Imaging and Medical Beam-Line. The individual microbeams were able to be resolved and the peak-to-valley dose ratio and the full width at half maximum of the microbeams was measured. These results are compared to a semiconductor strip detector of the same spatial resolution. A percent depth dose was measured and compared to data acquired by an ionisation chamber. The results presented demonstrate significant steps towards the development of an optical dosimeter with the potential to be applied in quality assurance of microbeam radiation therapy, which is vital if clinical trials are to be performed on human patients.

  4. Five Micron High Resolution MALDI Mass Spectrometry Imaging with Simple, Interchangeable, Multi-Resolution Optical System

    NASA Astrophysics Data System (ADS)

    Feenstra, Adam D.; Dueñas, Maria Emilia; Lee, Young Jin

    2017-03-01

    High-spatial resolution mass spectrometry imaging (MSI) is crucial for the mapping of chemical distributions at the cellular and subcellular level. In this work, we improved our previous laser optical system for matrix-assisted laser desorption ionization (MALDI)-MSI, from 9 μm practical laser spot size to a practical laser spot size of 4 μm, thereby allowing for 5 μm resolution imaging without oversampling. This is accomplished through a combination of spatial filtering, beam expansion, and reduction of the final focal length. Most importantly, the new laser optics system allows for simple modification of the spot size solely through the interchanging of the beam expander component. Using 10×, 5×, and no beam expander, we could routinely change between 4, 7, and 45 μm laser spot size, in less than 5 min. We applied this multi-resolution MALDI-MSI system to a single maize root tissue section with three different spatial resolutions of 5, 10, and 50 μm and compared the differences in imaging quality and signal sensitivity. We also demonstrated the difference in depth of focus between the optical systems with 10× and 5× beam expanders.

  5. Single-shot high-resolution characterization of optical pulses by spectral phase diversity

    SciTech Connect

    Dorrer, C.; Waxer, L. J.; Kalb, A.; Hill, E. M.; Bromage, J.

    2015-12-15

    The concept of spectral phase diversity is proposed and applied to the temporal characterization of optical pulses. The experimental trace is composed of the measured power of a plurality of ancillary optical pulses derived from the pulse under test by adding known amounts of chromatic dispersion. The spectral phase of the pulse under test is retrieved by minimizing the error between the experimental trace and a trace calculated from the optical spectrum using the known diagnostic parameters. An assembly composed of splitters and dispersive delay fibers has been used to generate 64 ancillary pulses whose instantaneous power can be detected in a single shot with a high-bandwidth photodiode and oscilloscope. Pulse-shape reconstruction for pulses shorter than the photodetection impulse response has been demonstrated.The diagnostic is experimentally shown to accurately characterize pulses from a chirped-pulse–amplification system when its stretcher is detuned from the position for optimal recompression. As a result, various investigations of the performance with respect to the number of ancillary pulses and the range of chromatic dispersion generated in the diagnostic are presented.

  6. High-resolution in vivo nanoparticle imaging using magnetomotive optical coherence tomography

    NASA Astrophysics Data System (ADS)

    Oldenburg, Amy L.; Luo, Wei; Boppart, Stephen A.

    2006-02-01

    In vivo imaging of the nanoparticle-tissue interaction reveals processes which aid in the improvement of disease-specific markers. Magnetomotive optical coherence tomography (MM-OCT) may fill this role by imaging magnetic nanoparticles (Fe 3O 4, 20-30nm diameter) similar to those currently used for MRI contrast. This is performed by modulating a small (<20mm) electromagnet during conventional OCT imaging and detecting the induced displacement (magnetomotion) of the nanoparticles. In a recent advance, increased specificity was achieved using a 3-pulse sequence to measure the intrinsic background fluctuation to normalize the magnetomotive signal. In this way ghosting due to physiological and Brownian motion are eliminated. Silicone tissue phantoms which are both optically and mechanically similar to soft human tissue were used to measure the scaling of the magnetomotive signal with magnetic field strength, local optical scattering efficiency, and magnetic nanoparticle concentration. MM-OCT is sensitive to magnetite nanoparticles at a concentration of 220μg/g (P>.975), with the possibility of detecting even lower concentrations (63μg/g) with minor improvements. The MM-OCT signal exhibits a gentler falloff in depth (~4dB over 0.5mm) than conventional OCT imaging, limited ultimately by shot noise. The performance of MM-OCT was evaluated in vivo in a Xenopus laevis tadpole exposed to magnetic nanoparticles for 24 hours prior to imaging. Corresponding histology demonstrates the ability to correctly identify regions of high nanoparticle concentration with in vivo MM-OCT.

  7. Development of micro-optics for high-resolution IL spectroscopy with a proton microbeam probe

    NASA Astrophysics Data System (ADS)

    Kada, Wataru; Satoh, Takahiro; Yokoyama, Akihito; Koka, Masashi; Kamiya, Tomihiro

    2014-01-01

    Confocal optics for ion luminescence (IL) was developed for the precise analysis of the chemical composition of microscopic targets with an external proton microbeam probe. Anti-reflection-coated confocal micro-lens optics with an effective focus area of approximately 800 × 800 μm was installed on the microbeam line of a single-ended accelerator. Chromatic aberrations of the confocal optics were examined at wavelengths of 300-900 nm. An electrically-cooled back-thinned charge coupled device spectrometer with a wavelength resolution of 0.5 nm was used for the microscopic spectroscopy and IL imaging of microscopic mineral targets. Simultaneous microscopic IL and micro-PIXE analysis were performed using an external 3 MeV H+ microbeam with a current of less than 100 pA. A spectral resolution of 3 nm was achieved for a single IL peak which corresponded to Cr3+ impurities in a single-crystal of aluminum oxide. The use of IL spectroscopy and imaging for aerosol targets revealed microscopic distributions of the chemical and elemental composition in the atmosphere.

  8. Single-shot high-resolution characterization of optical pulses by spectral phase diversity

    DOE PAGES

    Dorrer, C.; Waxer, L. J.; Kalb, A.; ...

    2015-12-15

    The concept of spectral phase diversity is proposed and applied to the temporal characterization of optical pulses. The experimental trace is composed of the measured power of a plurality of ancillary optical pulses derived from the pulse under test by adding known amounts of chromatic dispersion. The spectral phase of the pulse under test is retrieved by minimizing the error between the experimental trace and a trace calculated from the optical spectrum using the known diagnostic parameters. An assembly composed of splitters and dispersive delay fibers has been used to generate 64 ancillary pulses whose instantaneous power can be detectedmore » in a single shot with a high-bandwidth photodiode and oscilloscope. Pulse-shape reconstruction for pulses shorter than the photodetection impulse response has been demonstrated.The diagnostic is experimentally shown to accurately characterize pulses from a chirped-pulse–amplification system when its stretcher is detuned from the position for optimal recompression. As a result, various investigations of the performance with respect to the number of ancillary pulses and the range of chromatic dispersion generated in the diagnostic are presented.« less

  9. Five Micron High Resolution MALDI Mass Spectrometry Imaging with Simple, Interchangeable, Multi-Resolution Optical System

    DOE PAGES

    Feenstra, Adam D.; Dueñas, Maria Emilia; Lee, Young Jin

    2017-01-03

    High-spatial resolution mass spectrometry imaging (MSI) is crucial for the mapping of chemical distributions at the cellular and subcellular level. Here in this work, we improved our previous laser optical system for matrix-assisted laser desorption ionization (MALDI)-MSI, from ~9 μm practical laser spot size to a practical laser spot size of ~4 μm, thereby allowing for 5 μm resolution imaging without oversampling. This is accomplished through a combination of spatial filtering, beam expansion, and reduction of the final focal length. Most importantly, the new laser optics system allows for simple modification of the spot size solely through the interchanging ofmore » the beam expander component. Using 10×, 5×, and no beam expander, we could routinely change between ~4, ~7, and ~45 μm laser spot size, in less than 5 min. We applied this multi-resolution MALDI-MSI system to a single maize root tissue section with three different spatial resolutions of 5, 10, and 50 μm and compared the differences in imaging quality and signal sensitivity. Lastly, we also demonstrated the difference in depth of focus between the optical systems with 10× and 5× beam expanders.« less

  10. High resolution geochemical proxy record of the last 600yr in a speleothem from the northwest Iberian Peninsula

    NASA Astrophysics Data System (ADS)

    Iglesias González, Miguel; Pisonero, Jorge; Cheng, Hai; Edwards, R. Lawrence; Stoll, Heather

    2017-04-01

    In meteorology and climatology, the instrumental period is the period where we have measured directly by instrumentation, different meteorological data along the surface which allow us to determinate the evolution of the climate during the last 150 years over the world. At the beginning, the density of this data were very low, so we have to wait until the last 75-100 years to have a good network in most of the parts of the surface. This time period is very small if we want to analyze the relationship between geochemical and instrumental variability in any speleothem. So a very high resolution data is needed to determinate the connection between both of them in the instrumental period, to try to determinate de evolution of climate in the last 600 years. Here we present a high resolution speleothem record from a cave located in the middle of the Cantabrian Mountains without any anthropologic influence and with no CO2 seasonal variability. This 600yr stalagmite, dated with U/Th method with a growth rate from 100 to 200 micrometers/yr calculated with Bchron model, provide us accurate information of the climate conditions near the cave. Trace elements are analyzed at 8 micrometers intervals by Laser Ablation ICP-MS which resolves even monthly resolution during the last 600 years with special attention with Sr, Mg, Al and Si. This data, without seasonal variability and with the presence of a river inside the cave, give us very valuable information about the extreme flood events inside the cave during the whole period, which is related with the precipitations and the snow fusion events outside the cave. We identify more extremely flood events during the Little Ice Age than in the last 100yr. As well, we have trace elements data with spatial resolution of 0.2mm analyzed with ICP-AES which allow us to compare the geochemical variability with both technics. We also analyze stable isotope d13C and d18O with a spatial resolution of 0.2mm, so we are able to identify variations

  11. High Resolution, Multi-Proxy Records of Holocene Biomass Burning, Environmental Change, and Human Occupation in the Southern Maya Lowlands

    NASA Astrophysics Data System (ADS)

    Anderson, L.; Wahl, D.

    2011-12-01

    Understanding the relationship between the prehistoric Maya and their environment continues to be a primary research focus, particularly with respect to discerning the role of humans versus climate in driving environmental change. Fire was fundamental to prehistoric Maya architectural and agricultural land use practices. Burning was used to open forest for cultivation as well as for the construction of site centers and settlements. The production of lime plaster, and important building material, was dependent on significant amounts of green wood for kiln fuel. Large populations employing land use strategies dependent on burning would have put tremendous demands on forest resources. Despite the significance of fire in Maya pre-history, there has been no focused effort to produce records of biomass burning and its impacts. Here we present preliminary high-resolution fossil charcoal data that span the Holocene from a network of lacustrine and paludal sites across Peten, Guatemala. Charcoal influx data from the early to mid Holocene, prior to the arrival of sedentary agriculturalists, provides a baseline to infer natural fire regimes under specific climatic conditions, increasing our understanding of tropical fire ecology. Charcoal deposition that co-varies with evidence of agriculture and human activity can be attributed to anthropogenic burning. Results are synthesized with existing data (pollen, δ18O and δ13C, magnetic susceptibility, and physical properties) in an effort to understand the processes driving the location, timing, and extent of fires across the region. Placed in the context of changes in vegetation, sedimentation regime, and hydrology, these data provide new insight into topical fire ecology before the period of human occupation, as well as the dynamic relationship between the prehistoric Maya and their environment.

  12. High Resolution Trajectory-Based Smoke Forecasts Using VIIRS Aerosol Optical Depth and NUCAPS Carbon Monoxide Retrievals

    NASA Astrophysics Data System (ADS)

    Pierce, R. B.; Smith, N.; Barnet, C.; Barnet, C. D.; Kondragunta, S.; Davies, J. E.; Strabala, K.

    2016-12-01

    We use Suomi National Polar-orbiting Partnership (S-NPP) Visible Infrared Imaging Radiometer Suite (VIIRS) Aerosol Optical Depth (AOD) and combined Cross-track Infrared Sounder (CrIS) and Advanced Technology Microwave Sounder (ATMS) NOAA-Unique CrIS-ATMS Processing System (NUCAPS) carbon monoxide (CO) retrievals to initialize trajectory-based, high spatial resolution North American smoke dispersion forecasts during the May 2016 Fort McMurray wildfire in northern Alberta and the July 2016 Soberanes Fire in Northern California. These two case studies illustrate how long range transport of wild fire smoke can adversely impact surface air quality thousands of kilometers downwind and how local topographic flow can lead to complex transport patterns near the wildfire source region. The NUCAPS CO retrievals are shown to complement the high resolution VIIRS AOD retrievals by providing retrievals in partially cloudy scenes and also providing information on the vertical distribution of the wildfire smoke. This work addresses the need for low latency, web-based, high resolution forecasts of smoke dispersion for use by NWS Incident Meteorologists (IMET) to support on-site decision support services for fire incident management teams. The primary user community for the IDEA-I smoke forecasts is the Western regions of the NWS and US EPA due to the significant impacts of wildfires in these regions. Secondary users include Alaskan NWS offices and Western State and Local air quality management agencies such as the Western Regional Air Partnership (WRAP).

  13. Ultra High-Resolution Anterior Segment Optical Coherence Tomography in the Diagnosis and Management of Ocular Surface Squamous Neoplasia

    PubMed Central

    Thomas, Benjamin J.; Galor, Anat; Nanji, Afshan A.; Sayyad, Fouad El; Wang, Jianhua; Dubovy, Sander R.; Joag, Madhura G.; Karp, Carol L.

    2014-01-01

    The development of optical coherence tomography (OCT) technology has helped to usher in a new era of in vivo diagnostic imaging of the eye. The utilization of OCT for imaging of the anterior segment and ocular surface has evolved from time-domain devices to spectral-domain devices with greater penetrance and resolution, providing novel images of anterior segment pathology to assist in diagnosis and management of disease. Ocular surface squamous neoplasia (OSSN) is one such pathology that has proven demonstrable by certain anterior segment OCT machines, specifically the newer devices capable of performing ultra high-resolution OCT (UHR-OCT). Distinctive features of OSSN on high resolution OCT allow for diagnosis and differentiation from other ocular surface pathologies. Subtle findings on these images help to characterize the OSSN lesions beyond what is apparent with the clinical examination, providing guidance for clinical management. The purpose of this review is to examine the published literature on the utilization of UHR-OCT for the diagnosis and management of OSSN, as well as to report novel uses of this technology and potential directions for its future development. PMID:24439046

  14. The precision radial velocity error budget for the Gemini High-resolution Optical SpecTrograph (GHOST)

    NASA Astrophysics Data System (ADS)

    Ireland, Michael J.; Artigau, Étienne; Burley, Greg; Edgar, Michael; Margheim, Steve; Robertson, Gordon; Pazder, John; McDermid, Richard; Zhelem, Ross

    2016-08-01

    The Gemini High-resolution Optical SpecTrograph (GHOST) is a fiber fed spectrograph primarily designed for high efficiency and broad wavelength coverage (363 -1000nm), with an anticipated commissioning early in 2018. The primary scientific goal of the Precision Radial Velocity (PRV) mode will be follow-up of relatively faint (R>12) transiting exoplanet targets, especially from the TESS mission. In the PRV mode, the 1.2 arcsec diameter stellar image will be split 19 ways, combined in a single slit with a simultaneous Th/Xe reference source, dispersed at a resolving power of 80,000 and imaged onto two detectors. The spectrograph will be thermally stabilized in the Gemini pier laboratory, and modal noise will be reduced below other sources through the use of a fiber agitator. Unlike other precision high resolution spectrographs, GHOST will not be pressure controlled (although pressure will be monitored precisely), and there will be no double scrambler or shaped (e.g. octagonal) fibers. Instead, GHOST will have to rely on simultaneous two-color imaging of the slit and the simultaneous Th/Xe fiber to correct for variable fiber illumination and focal-ratio degradation. This configuration presents unique challenges in estimating a PRV error budget.

  15. A high-resolution record of Southern Ocean intermediate water radiocarbon over the past 30,000 years

    NASA Astrophysics Data System (ADS)

    Hines, Sophia K. V.; Southon, John R.; Adkins, Jess F.

    2015-12-01

    The circulation of intermediate waters plays an important role in global heat and carbon transport in the ocean and changes in their distribution are closely tied to glacial-interglacial climate change. Coupled radiocarbon and U/Th measurements on deep-sea Desmophyllum dianthus corals allow for the reconstruction of past intermediate water ventilation. We present a high-resolution time series of Antarctic Intermediate Water radiocarbon from 44 corals spanning 30 ka through the start of the Holocene, encompassing the transition into the Last Glacial Maximum (LGM) and the last deglaciation. Corals were collected south of Tasmania from water depths between 1430 and 1950 m with 80% of them between 1500 and 1700 m, giving us a continuous record from a narrow depth range. The record shows three distinct periods of circulation: the MIS 3-2 transition, the LGM/Heinrich Stadial 1 (extending from ∼22 to 16 kyr BP), and the Antarctic Cold Reversal (ACR). The MIS 3-2 transition and the ACR are characterized by abrupt changes in intermediate water radiocarbon while the LGM time period generally follows the atmosphere at a constant offset, in support of the idea that the LGM ocean was at steady state for its 14C distribution. Closer inspection of the LGM time period reveals a 40‰ jump at ∼19 ka from an atmospheric offset of roughly 230‰ to 190‰, coincident with an observed 10-15 m rise in sea level and a southward shift of the Subantarctic and Polar Fronts, an abrupt change not seen in deeper records. During the ACR time period intermediate water radiocarbon is on average less offset from the atmosphere (∼ 110 ‰) and much more variable. This variability has been captured within the lifetimes of three individual corals with changes of up to 35‰ over ∼40 yr, likely caused by the movement of Southern Ocean fronts. This surprising result of relatively young and variable intermediate water radiocarbon during the ACR seems to go against the canonical idea of reduced

  16. High-Resolution Speleothem Records of Climate Variability from the Northwestern Iberian Peninsula During the Last 30,000 Years

    NASA Astrophysics Data System (ADS)

    Moreno, A.; Stoll, H.; Jiménez-Sánchez, M.; Valero-Garcés, B. L.; Edwards, L.; Ito, E.; Vadillo, I.; Trigo, R. M.; Cacho, I.

    2007-12-01

    hydrological and soil-vegetation patterns of variation. Our high resolution (48 hour) analysis of oxygen isotopes in modern precipitation at the site reveals a large (6 ‰) range which is highly correlated with the daily CPC NAO index and only weakly correlated with air temperature or precipitation amount. Oxygen isotopes in rain and dripwater might thus be most strongly regulated by the dynamics of precipitation (moisture source and condensation height and temperature), superimposed on the trend in δ18O of seawater from ice volume effects. Thus, the negative isotopic excursion during the Younger Dryas and those events during the Holocene could reflect precipitation generated under conditions more similar to those characterizing the modern negative NAO phase. Mg/Ca ratios rise sharply after 8000 years BP likely reflecting sea level rise and greater proximity to the coast which increases sea salt aerosol Mg deposition.

  17. Optical depth of the Martian atmosphere and surface albedo from high-resolution orbiter images

    NASA Astrophysics Data System (ADS)

    Petrova, E. V.; Hoekzema, N. M.; Markiewicz, W. J.; Thomas, N.; Stenzel, O. J.

    2012-01-01

    In this paper we describe and evaluate the so-called shadow method. This method can be used to estimate the optical depth of the Martian atmosphere from the differences in brightness between shadowed and sunlit regions observed from an orbiter. We present elaborate and simplified versions of the method and analyze the capabilities and the sources of errors. It proves essential to choose shadowed and sunlit comparison regions with similar surface properties. Accurate knowledge of the observing geometry, including the slopes of the observed region, is important as well, since the procedure should be corrected for the non-horizontal surface. Moreover, the elaborate version of the shadow method can be sensitive to (i) the optical model of aerosols and (ii) the assumed bi-directional reflectance function of the surface. To obtain reliable estimates, the analyzed images must have a high spatial resolution, which the HiRISE camera onboard the MRO provides. We tested the shadow method on two HiRISE images of Victoria crater (TRA_0873_1780 and PSP_001414_1780) that were taken while this crater was the exploration site of the Opportunity rover. While the rover measured optical depth τ approximately in the ranges from 0.43 to 0.53 and from 0.53 to 0.59 by imaging the sun, our shadow procedure yielded τ about 0.50 and 0.575, respectively (from the HiRISE's red images). Thus, the agreement is quite good. The obtained estimates of the surface albedo are about 0.20 and 0.17, respectively.

  18. High-resolution, long-term characterization of bacterial motility using optical tweezers

    PubMed Central

    Min, Taejin L.; Mears, Patrick J.; Chubiz, Lon M.; Rao, Christopher V.; Golding, Ido; Chemla, Yann R.

    2009-01-01

    We present a single-cell motility assay, which allows the quantification of bacterial swimming in a well-controlled environment, for durations of up to an hour and with a temporal resolution higher than the flagellar rotation rates of ~100 Hz. The assay is based on an instrument combining optical tweezers, light and fluorescence microscopy, and a microfluidic chamber. Using this device we characterized the long-term statistics of the run-tumble time series in individual Escherichia coli cells. We also quantified higher-order features of bacterial swimming, such as changes in velocity and reversals of swimming direction. PMID:19801991

  19. High-resolution in vivo optical imaging of stroke injury and repair

    PubMed Central

    Sakadžić, Sava; Lee, Jonghwan; Boas, David A.; Ayata, Cenk

    2015-01-01

    Central nervous system (CNS) function and dysfunction are best understood within a framework of interactions between neuronal, glial and vascular compartments comprising the neurovascular unit (NVU), all of which contribute to stroke-induced CNS injury, plasticity, repair, and recovery. Recent advances in in vivo optical microscopy have enabled us to observe and interrogate cells and their processes with high spatial resolution in real time and in their natural environment deep in the brain tissue. Here, we review some of these state-of-the-art imaging techniques with an emphasis on imaging the interactions among the constituents of the NVU during ischemic injury and repair in small animal models. PMID:25960347

  20. Optical scanning holography as a technique for high-resolution three-dimensional biological microscopy

    NASA Astrophysics Data System (ADS)

    Swoger, Jim; Martinez-Corral, Manuel; Huisken, Jan; Stelzer, Ernst H. K.

    2002-09-01

    The applicability of optical scanning holography (OSH) to the field of microscopic imaging for biological applications is assessed. A generalized mathematical description of OSH that takes into account polarization effects, high numerical apertures, and generalized illumination wave fronts is presented. This description is used to show that the proposed single-beam scanning technique relaxes the restrictions under which OSH functions correctly compared with the conventional double-beam scanning method. It is also shown that, although in general OSH is restricted to thin samples, this condition can be relaxed in nonrefracting fluorescence samples, which are of importance in biological microscopy.

  1. High resolution kilometric range optical telemetry in air by radio frequency phase measurement

    NASA Astrophysics Data System (ADS)

    Guillory, Joffray; Šmíd, Radek; García-Márquez, Jorge; Truong, Daniel; Alexandre, Christophe; Wallerand, Jean-Pierre

    2016-07-01

    We have developed an optical Absolute Distance Meter (ADM) based on the measurement of the phase accumulated by a Radio Frequency wave during its propagation in the air by a laser beam. In this article, the ADM principle will be described and the main results will be presented. In particular, we will emphasize how the choice of an appropriate photodetector can significantly improve the telemeter performances by minimizing the amplitude to phase conversion. Our prototype, tested in the field, has proven its efficiency with a resolution better than 15 μm for a measurement time of 10 ms and distances up to 1.2 km.

  2. Compact, low-cost, and high-resolution interrogation unit for optical sensors

    SciTech Connect

    Kiesel, Peter; Schmidt, Oliver; Mohta, Setu; Johnson, Noble; Malzer, Stefan

    2006-11-13

    Compact wavelength detectors that resolve wavelength changes in the subpicometer range over a broad spectral range are presented. A photodiode array or position sensor device is coated with a linear variable filter that converts the wavelength of the incident light into a spatial intensity distribution. The centroid of the spatial distribution is determined by a differential readout of the two elements of the photodiode array or the position sensor device. The device can interrogate any optical sensor that produces a wavelength shift in response to a stimulus. The potential of this device was tested by interrogating fiber-Bragg-grating sensors.

  3. High-resolution adaptive optics scanning laser ophthalmoscope with multiple deformable mirrors

    DOEpatents

    Chen, Diana C.; Olivier, Scot S.; Jones; Steven M.

    2010-02-23

    An adaptive optics scanning laser ophthalmoscopes is introduced to produce non-invasive views of the human retina. The use of dual deformable mirrors improved the dynamic range for correction of the wavefront aberrations compared with the use of the MEMS mirror alone, and improved the quality of the wavefront correction compared with the use of the bimorph mirror alone. The large-stroke bimorph deformable mirror improved the capability for axial sectioning with the confocal imaging system by providing an easier way to move the focus axially through different layers of the retina.

  4. High resolution kilometric range optical telemetry in air by radio frequency phase measurement

    SciTech Connect

    Guillory, Joffray; García-Márquez, Jorge; Truong, Daniel; Wallerand, Jean-Pierre; Šmíd, Radek; Alexandre, Christophe

    2016-07-15

    We have developed an optical Absolute Distance Meter (ADM) based on the measurement of the phase accumulated by a Radio Frequency wave during its propagation in the air by a laser beam. In this article, the ADM principle will be described and the main results will be presented. In particular, we will emphasize how the choice of an appropriate photodetector can significantly improve the telemeter performances by minimizing the amplitude to phase conversion. Our prototype, tested in the field, has proven its efficiency with a resolution better than 15 μm for a measurement time of 10 ms and distances up to 1.2 km.

  5. Quasi-microscope concept for planetary missions. [optically augmented lander camera for high resolution microscopy

    NASA Technical Reports Server (NTRS)

    Huck, F. O.; Burcher, E. E.; Wall, S. D.; Arvidson, R. E.; Giat, O.

    1977-01-01

    Viking lander cameras have returned stereo and multispectral views of the Martian surface with a resolution that approaches 2 mm/lp in the near field. A two-orders-of-magnitude increase in resolution could be obtained for collected surface samples by augmenting these cameras with auxiliary optics that would neither impose special camera design requirements nor limit the cameras field of view of the terrain. Quasi-microscope images would provide valuable data on the physical and chemical characteristics of planetary regoliths.

  6. High resolution on-chip optical filter array based on double subwavelength grating reflectors

    SciTech Connect

    Horie, Yu; Arbabi, Amir; Han, Seunghoon; Faraon, Andrei

    2015-11-05

    An optical filter array consisting of vertical narrow-band Fabry-Pèrot (FP) resonators formed by two highly reflective high contrast subwavelength grating mirrors is reported. The filters are designed to cover a wide range of operation wavelengths ( Δλ/λ=5%) just by changing the in-plane grating parameters while the device thickness is maintained constant. In conclusion, operation in the telecom band with transmission efficiencies greater than 40% and quality factors greater than 1,000 are measured experimentally for filters fabricated on the same substrate.

  7. Ultra-high sensitive and high resolution optical coherence tomography using a laser induced electromagnetic dipole

    NASA Astrophysics Data System (ADS)

    Kuroda, Hiroto; Baba, Motoyoshi; Suzuki, Masayuki; Yoneya, Shin

    2013-09-01

    Utilizing an optical coherence tomography measurement, we have developed a technique that actively uses a dielectric response due to an induced dipole moment caused by a mode-locked pulsed laser light source. Irradiated laser light in the material induces a photo-induced electric dipole resulting in a refractive index change for its strong electric field. Using this technique, we obtained a highly sensitive fundus retina tomogram of a human eye in vivo with a resolution of 1.3 μm by single scanning for 20 ms using 8 fs mode-locked pulse laser light with a 350 nm spread spectrum.

  8. Ultra-high resolution optical CT dosimetry for the visualisation of synchrotron microbeam therapy doses

    NASA Astrophysics Data System (ADS)

    Doran, S. J.; Rahman, A. T. Abdul; Bräuer-Krisch, E.; Brochard, T.; Adamovics, J.

    2013-06-01

    Optical CT is a method that can potentially provide both accurate dosimetry at high spatial resolution and 3-D visualisation over a large field-of-view in a single dataset. The major factors limiting spatial resolution in previous studies are analysed here and it is shown that improvements in equipment specification can overcome many of these. The need for ultra-high spatial resolution in the verification of microbeam radiation therapy verification is demonstrated and example images of a PRESAGE® sample are presented.

  9. A simple fiber-optic microprobe for high resolution light measurements: application in marine sediment

    NASA Technical Reports Server (NTRS)

    Jorgensen, B. B.; Des Marais, D. J.

    1986-01-01

    A fiber-optic microphobe is described which is inexpensive and simple to build and use. It consists of an 80-micrometers optical fiber which at the end is tapered down to a rounded sensing tip of 20-30-micrometers diameter. The detector is a hybrid photodiode/amplifier. The probe has a sensitivity of 0.01 microEinst m-2 s-1 and a spectral range of 300-1,100 nm. Spectral light gradients were measured in fine-grained San Francisco Bay sediment that had an undisturbed diatom coating on the surface. The photic zone of the mud was only 0.4 mm deep. Measured in situ spectra showed extinction maxima at 430-520, 620-630, 670, and 825-850 nm due to absorption by chlorophyll a, carotenoids, phycocyanin, and bacterio-chlorophyll a. Maximum light penetration in the visible range was found in both the violet and the red < or = 400 and > or = 700 nm.

  10. High-Resolution Adaptive Optics Scanning Laser Ophthalmoscope with Dual Deformable Mirrors

    SciTech Connect

    Chen, D C; Jones, S M; Silva, D A; Olivier, S S

    2006-08-11

    Adaptive optics scanning laser ophthalmoscope (AO SLO) has demonstrated superior optical quality of non-invasive view of the living retina, but with limited capability of aberration compensation. In this paper, we demonstrate that the use of dual deformable mirrors can effectively compensate large aberrations in the human retina. We used a bimorph mirror to correct large-stroke, low-order aberrations and a MEMS mirror to correct low-stroke, high-order aberration. The measured ocular RMS wavefront error of a test subject was 240 nm without AO compensation. We were able to reduce the RMS wavefront error to 90 nm in clinical settings using one deformable mirror for the phase compensation and further reduced the wavefront error to 48 nm using two deformable mirrors. Compared with that of a single-deformable-mirror SLO system, dual AO SLO offers much improved dynamic range and better correction of the wavefront aberrations. The use of large-stroke deformable mirrors provided the system with the capability of axial sectioning different layers of the retina. We have achieved diffraction-limited in-vivo retinal images of targeted retinal layers such as photoreceptor layer, blood vessel layer and nerve fiber layers with the combined phase compensation of the two deformable mirrors in the AO SLO.

  11. Adaptive optics for high resolution spectroscopy: a direct application with the future NIRPS spectrograph

    NASA Astrophysics Data System (ADS)

    Conod, U.; Blind, N.; Wildi, F.; Pepe, F.

    2016-07-01

    Radial velocity instruments require high spectral resolution and extreme thermo-mechanical stability, even more difficult to achieve in near-infra red (NIR) where the spectrograph has to be cooled down. For a seeing-limited spectrograph, the price of high spectral resolution is an increased instrument volume, proportional to the diameter of the primary mirror. A way to control the size, cost, and stability of radial velocity spectrographs is to reduce the beam optical etendue thanks to an Adaptive Optics (AO) system. While AO has revolutionized the field of high angular resolution and high contrast imaging during the last 20 years, it has not yet been (successfully) used as a way to control spectrographs size, especially in the field of radial velocities. In this work we present the AO module of the future NIRPS spectrograph for the ESO 3.6 m telescope, that will be feed with multi-mode fibers. We converge to an AO system using a Shack-Hartmann wavefront sensor with 14x14 subapertures, able to feed 50% of the energy into a 0.4" fiber in the range of 0.98 to 1.8 μm for M-type stars as faint as I=12.

  12. High-resolution all-optical photoacoustic imaging system for remote interrogation of biological specimens

    NASA Astrophysics Data System (ADS)

    Sampathkumar, Ashwin

    2014-05-01

    Conventional photoacoustic imaging (PAI) employs light pulses to produce a photoacoustic (PA) effect and detects the resulting acoustic waves using an ultrasound transducer acoustically coupled to the target tissue. The resolution of conventional PAI is limited by the sensitivity and bandwidth of the ultrasound transducer. We have developed an all-optical versatile PAI system for characterizing ex vivo and in vivo biological specimens. The system employs noncontact interferometric detection of the acoustic signals that overcomes limitations of conventional PAI. A 532-nm pump laser with a pulse duration of 5 ns excited the PA effect in tissue. Resulting acoustic waves produced surface displacements that were sensed using a 532-nm continuous-wave (CW) probe laser in a Michelson interferometer with a GHz bandwidth. The pump and probe beams were coaxially focused using a 50X objective giving a diffraction-limited spot size of 0.48 μm. The phase-encoded probe beam was demodulated using a homodyne interferometer. The detected time-domain signal was time reversed using k-space wave-propagation methods to produce a spatial distribution of PA sources in the target tissue. Performance was assessed using PA images of ex vivo rabbit lymph node specimens and human tooth samples. A minimum peak surface displacement sensitivity of 0.19 pm was measured. The all-optical PAI (AOPAI) system is well suited for assessment of retinal diseases, caries lesion detection, skin burns, section less histology and pressure or friction ulcers.

  13. A simple fiber-optic microprobe for high resolution light measurements: application in marine sediment

    NASA Technical Reports Server (NTRS)

    Jorgensen, B. B.; Des Marais, D. J.

    1986-01-01

    A fiber-optic microphobe is described which is inexpensive and simple to build and use. It consists of an 80-micrometers optical fiber which at the end is tapered down to a rounded sensing tip of 20-30-micrometers diameter. The detector is a hybrid photodiode/amplifier. The probe has a sensitivity of 0.01 microEinst m-2 s-1 and a spectral range of 300-1,100 nm. Spectral light gradients were measured in fine-grained San Francisco Bay sediment that had an undisturbed diatom coating on the surface. The photic zone of the mud was only 0.4 mm deep. Measured in situ spectra showed extinction maxima at 430-520, 620-630, 670, and 825-850 nm due to absorption by chlorophyll a, carotenoids, phycocyanin, and bacterio-chlorophyll a. Maximum light penetration in the visible range was found in both the violet and the red < or = 400 and > or = 700 nm.

  14. A New High-Resolution Record of Benthic and Planktonic Foraminiferal Stable Isotopes from the Bermuda Rise

    NASA Astrophysics Data System (ADS)

    Curry, W. B.; Keigwin, L. D.

    2011-12-01

    A new core from Bermuda Rise (KNR191-1-19CDH, 33° 41.194' N, 57° 36.907' W, 4579 meters) recovered 38 m of sediment spanning the last 160,000 years. We have now completed initial high-resolution records of planktonic (G. ruber, 212-250μm) and benthic (C. wuellerstorfi, C. sp., and N. umbonifera, for the most part) foraminiferal stable isotopes for the last deglaciation using samples spaced every centimeter, providing a median sample spacing of about 110 to 130 years. Because of the high and variable sedimentation rates, the foraminifera are not always present in each sample so the mean sample spacing in each time series is a bit higher. The age model for this core is based on many new AMS 14C dates on G. inflata, and additional age control provided by correlation to other well-dated cores nearby. The records are higher resolution reproductions of several previous studies, all accomplished on different Bermuda Rise sediment cores. The G. ruber δ18O record exhibits a three-stage decrease during the deglaciation. An initial decrease of about 1% occurs at 16.5 ka and is followed by a return to near glacial values by 15.5 ka. The next decrease of about 1.5% begins at about 14.6 ka and is nearly synchronous with the Bolling-Allerod warming observed in the Greenland ice cores. A Younger Dryas cooling, observed as a 0.5% increase in δ18O, is followed by a warming into the early Holocene where oxygen isotope values are about 2% lower than glacial maximum values. The benthic foraminiferal carbon isotope record is unprecendented in its detail for Bermuda Rise cores. Last glacial maximum values are variable but average about -0.5% (VPDB). There is an increase to higher values at about 16.5 ka - synchronous with the first decrease in G. ruber δ18O - and a return to very low values (-0.7 to -0.8% VPDB) by about 15.5 ka. The record exhibits an abrupt 1% increase in δ13C beginning at about 14.4 ka, followed by a 0.5% decrease during the Younger Dryas. Maximum values occur

  15. Flexible, high-resolution micro-optical coherence tomography endobronchial probe toward in vivo imaging of cilia.

    PubMed

    Cui, Dongyao; Chu, Kengyeh K; Yin, Biwei; Ford, Timothy N; Hyun, Chulho; Leung, Hui Min; Gardecki, Joseph A; Solomon, George M; Birket, Susan E; Liu, Linbo; Rowe, Steven M; Tearney, Guillermo J

    2017-02-15

    We report the design and fabrication of a flexible, longitudinally scanning high-resolution micro-optical coherence tomography (μOCT) endobronchial probe, optimized for micro-anatomical imaging in airways. The 2.4 mm diameter and flexibility of the probe allows it to be inserted into the instrument channel of a standard bronchoscope, enabling real-time video guidance of probe placement. To generate a depth-of-focus enhancing annular beam, we utilized a new fabrication method, whereby a hollow glass ferrule was angle-polished and gold-coated to produce an elongated annular reflector. We present validation data that verifies the preservation of linear scanning, despite the use of flexible materials. When utilized on excised, cultured mouse trachea, the probe acquired images of comparable quality to those obtained by a benchtop μOCT system.

  16. Ultra-high resolution polarization-sensitive optical coherence tomography for imaging of the retinal nerve fiber layer

    NASA Astrophysics Data System (ADS)

    Cense, Barry; Reddikumar, Maddipatla; Cervantes, Joel

    2017-04-01

    A spectrometer design with a multiple line line-scan camera and beam displacer is presented for ultra-high resolution optical coherence tomography measurements of the human retina at 840 nm. The beam displacer offsets the two orthogonal polarization states on the same line-scan camera, which reduces k-space mapping complexity, as data in both polarization channels can be mapped with the same procedure. Its coherence length is 2.8 μm in tissue (n = 1.38). Birefringence values of 1°/μm and higher were found in a circle with a radius of 2.5° eccentricity centered on the fovea, and in the raphe, pointing at a higher packing density of microtubules and a lower concentration of glia. Birefringence measurements may be more helpful in the modeling of individual structure-function maps than thickness measurements, as they are not affected by glial content.

  17. Moving-source elastic wave reconstruction for high-resolution optical coherence elastography

    NASA Astrophysics Data System (ADS)

    Hsieh, Bao-Yu; Song, Shaozhen; Nguyen, Thu-Mai; Yoon, Soon Joon; Shen, Tueng T.; Wang, Ruikang K.; O'Donnell, Matthew

    2016-11-01

    Optical coherence tomography (OCT)-based elasticity imaging can map soft tissue elasticity based on speckle-tracking of elastic wave propagation using highly sensitive phase measurements of OCT signals. Using a fixed elastic wave source and moving detection, current imaging sequences have difficulty in reconstructing tissue elasticity within speckle-free regions, for example, within the crystalline lens of the eye. We present a moving acoustic radiation force imaging sequence to reconstruct elastic properties within a speckle-free region by tracking elastic wave propagation from multiple laterally moving sources across the field of view. We demonstrate the proposed strategy using heterogeneous and partial speckle-free tissue-mimicking phantoms. Harder inclusions within the speckle-free region can be detected, and the contrast-to-noise ratio slightly enhanced compared to current OCE imaging sequences. The results suggest that a moving source approach may be appropriate for OCE studies within the large speckle-free regions of the crystalline lens.

  18. Mirrors for High Resolution X-Ray Optics---Figure Preserving IR/PT Coating

    NASA Technical Reports Server (NTRS)

    Chan, Kai-Wing; Olsen, Lawrence; Sharpe, Marton; Numata, Ai; McClelland, Ryan; Saha, Timo; Zhang, Will

    2016-01-01

    Coating stress of 10 - 20 nm of Ir is sufficiently high to distort the figure of arc-second thin lightweight mirrors. For iridium: --Stress sigma 4 GPa for 15 nm film implies 60 Nm integrated stress-- Need less than 3 N/m (or stress less than 200 MPa) for sub-arcsecond optics. Basic Approaches for Mitigation. A. Annealing the film-- Glass can be heat up to 400 C without distortion. Silicon is even more resistant.-- It was found that recovery is limited by residual thermal stress from taking the mirror down from high T. B. Coating bi-layer films with compressive stress with tensile stress. C. Front-and-back coating with magnetron sputtering or atomic layer deposition-- Sputtering involve spanning of substrates. Geometric difference in setup (convexness/concaveness of curved mirrors) does not permit precise front-and-back matching-- Atomic layer deposition can provide a uniform deposition front and back simultaneously.

  19. COSMIC: A high resolution, large collecting area telescope. [Coherent Optical System of Modular Imaging Collectors (COSMIC)

    NASA Technical Reports Server (NTRS)

    Traub, W. A.; Carleton, N. P.

    1985-01-01

    The spaceborne Coherent Optical System of Modular Imaging Collectors (COSMIC) is presented. It has high angular resolution and can produce images of complex, low-surface-brightness objects such as distant galaxies. If configured as a 36 m filled linear array, COSMIC can have 15 times better angular resolution and 10 times greater collecting area than the Space Telescope. Alternatively, if the collecting area is spread out to create an unfilled two-dimensional array, there is the additional advantage of not needing to rotate the array in order to build up a reconstructed image. Considerations which led to the design concept, scientific goals, and the potentially useful role of a space station for assembly are discussed.

  20. High resolution spectroscopy of Cs vapor confined in optical cells of few-micron thicknesses

    NASA Astrophysics Data System (ADS)

    Cartaleva, S.; Krasteva, A.; Sargsyan, A.; Sarkisyan, D.; Slavov, D.; Vartanyan, T.

    2012-12-01

    We present here the new behavior of Electromagnetically Induced Transparency (EIT), Velocity Selective Optical Pumping (VSOP) and Velocity Selective Excitation (VSE) resonances observed in Cs vapor confined in unique cells with thicknesses L = 1.5λ and L = 6λ. It is shown experimentally that in both cells, the EIT resonance is significantly narrower than would be expected from the ground state dephasing rate due to atomic collisions with the cell windows. The enhanced absorption (fluorescence) narrow VSOP resonance at the closed transition transforms into reduced absorption (fluorescence) one with small increase of atomic concentration or light intensity. A striking difference appears between the VSE resonance broadening due to excited atom thermalization, in L = 6λ and conventional L = 2.5 cm cells.

  1. High Resolution Phase-Sensitive Magnetomotive Optical Coherence Microscopy for Tracking Magnetic Microbeads and Cellular Mechanics

    PubMed Central

    Crecea, Vasilica; Graf, Benedikt W.; Kim, Taewoo; Popescu, Gabriel; Boppart, Stephen A.

    2014-01-01

    We present a real-time multimodal near-infrared imaging technology that tracks externally induced axial motion of magnetic microbeads in single cells in culture. The integrated multimodal imaging technique consists of phase-sensitive magnetomotive optical coherence microscopy (MM-OCM) and multiphoton microscopy (MPM).MPMis utilized for the visualization of multifunctional fluorescent and magnetic microbeads, while MM-OCM detects, with nanometer-scale sensitivity, periodic displacements of the microbeads induced by the modulation of an external magnetic field. Magnetomotive signals are measured from mouse macrophages, human breast primary ductal carcinoma cells, and human breast epithelial cells in culture, and validated with full-field phase-sensitive microscopy. This methodology demonstrates the capability for imaging controlled cell dynamics and has the potential for measuring cell biomechanical properties, which are important in assessing the health and pathological state of cells. PMID:25400496

  2. High resolution coherence domain depth-resolved nailfold capillaroscopy based on correlation mapping optical coherence tomography

    NASA Astrophysics Data System (ADS)

    Subhash, Hrebesh M.; O'Gorman, Sean; Neuhaus, Kai; Leahy, Martin

    2014-03-01

    In this paper we demonstrate a novel application of correlation mapping optical coherence tomography (cm-OCT) for volumetric nailfold capillaroscopy (NFC). NFC is a widely used non-invasive diagnostic method to analyze capillary morphology and microvascular abnormalities of nailfold area for a range of disease conditions. However, the conventional NFC is incapable of providing volumetric imaging, when volumetric quantitative microangiopathic parameters such as plexus morphology, capillary density, and morphologic anomalies of the end row loops most critical. cm-OCT is a recently developed well established coherence domain magnitude based angiographic modality, which takes advantage of the time-varying speckle effect, which is normally dominant in the vicinity of vascular regions compared to static tissue region. It utilizes the correlation coefficient as a direct measurement of decorrelation between two adjacent B-frames to enhance the visibility of depth-resolved microcirculation.

  3. Low frequency Raman scattering for high resolution low temperature optical fiber sensors

    NASA Astrophysics Data System (ADS)

    Rabia, M. K.; Jurdyc, A.-M.; Le Brusq, J.; Champagnon, B.; Vouagner, D.

    2017-09-01

    Raman distributed optical fiber temperature sensors are based on the intensity ratio of the anti-Stokes to the Stokes Raman band at 440 cm-1 of silica. In this paper we predict that the sensitivity of the Raman measurements for low temperatures can be improved by considering the Boson peak in the low frequency Raman scattering domain at 60 cm-1. In this way Raman temperature sensors can be performed down to cryogenic temperatures. It is further shown that the Boson peak is less dependent than the 440 cm-1 band to the polarization of light. For the usual excitation at 1550 nm the anti-Stokes Boson peak at 1536 nm is in the low loss transmission window of the silica fibers.

  4. High-resolution optical and ultraviolet absorption-line studies of interstellar gas

    NASA Technical Reports Server (NTRS)

    Cowie, Lennox L.; Songaila, Antoinette

    1986-01-01

    Recent progress in the characterization of the interstellar medium (ISM) by means of optical and UV spectral data is summarized. The gas is studied by focusing on background stars whose spectra can be accurately modeled to provide the light source for the absorption-line scans. The capabilities of earth- and space-based instruments which have been and are used for the surveys are delineated. The distributions of diffuse gas densities and characteristics of the cold, warm and hot gas in the Galaxy are described in terms of the elemental abundances, kinetics and distributions of the gas. Particular note is taken of gas in the solar neighborhood and around SNR, and of absorption-line data of cosmological significance.

  5. High-Resolution and Lightweight X-ray Optics for the X-Ray Surveyor

    NASA Astrophysics Data System (ADS)

    Zhang, William

    Envisioned in "Enduring Quest, Daring Visions" and under study by NASA as a potential major mission for the 2020s, the X-ray Surveyor mission will likely impose three requirements on its optics: (1) high angular resolution: 0.5 PSF, (2) large effective area: e10,000 cm2 or more, and (3) affordable production cost: $500M. We propose a technology that can meet these requirements by 2020. It will help the X-ray Surveyor secure the endorsement of the coming decadal survey and enable its implementation following WFIRST. The technology comprises four elements: (1) fabrication of lightweight single crystal silicon mirrors, (2) coating these mirrors with iridium to maximize effective area without figure degradation, (3) alignment and bonding of these mirrors to form meta-shells that will be integrated to make a mirror assembly, and (4) systems engineering to ensure that the mirror assembly meet all science performance and spaceflight environmental requirements. This approach grows out of our existing approach based on glass slumping. Using glass slumping technology, we have been able to routinely build and test mirror modules of 10half-power diameter (HPD). While comparable in HPD to XMM-Newtons electroformed nickel mirrors, these mirror modules are 10 times lighter. Likewise, while comparable in weight to Suzakus epoxy-replicated aluminum foil mirrors, these modules have 10 times better HPD. These modules represent the current state of the art of lightweight X-ray optics. Although both successful and mature, the glass slumping technology has reached its limit and cannot achieve sub-arc second HPD. Therefore, we are pursuing the new approach based on polishing single crystal silicon. The new approach will enable the building and testing of mirror modules, called meta-shells, capable of 3HPD by 2018 and 1HPD by 2020, and has the potential to reach diffraction limits ( 0.1) in the 2020s.

  6. Affordable and lightweight high-resolution x-ray optics for astronomical missions

    NASA Astrophysics Data System (ADS)

    Zhang, W. W.; Biskach, M. P.; Bly, V. T.; Carter, J. M.; Chan, K. W.; Gaskin, J. A.; Hong, M.; Hohl, B. R.; Jones, W. D.; Kolodziejczak, J. J.; Kolos, L. D.; Mazzarella, J. R.; McClelland, R. S.; McKeon, K. P.; Miller, T. M.; O'Dell, S. L.; Riveros, R. E.; Saha, T. T.; Schofield, M. J.; Sharpe, M. V.; Smith, H. C.

    2014-07-01

    Future x-ray astronomical missions require x-ray mirror assemblies that provide both high angular resolution and large photon collecting area. In addition, as x-ray astronomy undertakes more sensitive sky surveys, a large field of view is becoming increasingly important as well. Since implementation of these requirements must be carried out in broad political and economical contexts, any technology that meets these performance requirements must also be financially affordable and can be implemented on a reasonable schedule. In this paper we report on progress of an x-ray optics development program that has been designed to address all of these requirements. The program adopts the segmented optical design, thereby is capable of making both small and large mirror assemblies for missions of any size. This program has five technical elements: (1) fabrication of mirror substrates, (2) coating, (3) alignment, (4) bonding, and (5) mirror module systems engineering and testing. In the past year we have made progress in each of these five areas, advancing the angular resolution of mirror modules from 10.8 arc-seconds half-power diameter reported (HPD) a year ago to 8.3 arc-seconds now. These mirror modules have been subjected to and passed all environmental tests, including vibration, acoustic, and thermal vacuum. As such this technology is ready for implementing a mission that requires a 10-arc-second mirror assembly. Further development in the next two years would make it ready for a mission requiring a 5-arc-second mirror assembly. We expect that, by the end of this decade, this technology would enable the x-ray astrophysical community to compete effectively for a major x-ray mission in the 2020s that would require one or more 1-arc-second mirror assemblies for imaging, spectroscopic, timing, and survey studies.

  7. Affordable and Lightweight High-Resolution X-ray Optics for Astronomical Missions

    NASA Technical Reports Server (NTRS)

    Zhang, W. W.; Biskach, M. P.; Bly, V. T.; Carter, J. M.; Chan, K. W.; Gaskin, J. A.; Hong, M.; Hohl, B. R.; Jones, W. D.; Kolodziejczak, J. J.

    2014-01-01

    Future x-ray astronomical missions require x-ray mirror assemblies that provide both high angular resolution and large photon collecting area. In addition, as x-ray astronomy undertakes more sensitive sky surveys, a large field of view is becoming increasingly important as well. Since implementation of these requirements must be carried out in broad political and economical contexts, any technology that meets these performance requirements must also be financially affordable and can be implemented on a reasonable schedule. In this paper we report on progress of an x-ray optics development program that has been designed to address all of these requirements. The program adopts the segmented optical design, thereby is capable of making both small and large mirror assemblies for missions of any size. This program has five technical elements: (1) fabrication of mirror substrates, (2) coating, (3) alignment, (4) bonding, and (5) mirror module systems engineering and testing. In the past year we have made progress in each of these five areas, advancing the angular resolution of mirror modules from 10.8 arc-seconds half-power diameter reported (HPD) a year ago to 8.3 arc-seconds now. These mirror modules have been subjected to and passed all environmental tests, including vibration, acoustic, and thermal vacuum. As such this technology is ready for implementing a mission that requires a 10-arc-second mirror assembly. Further development in the next two years would make it ready for a mission requiring a 5-arc-second mirror assembly. We expect that, by the end of this decade, this technology would enable the x-ray astrophysical community to compete effectively for a major x-ray mission in the 2020s that would require one or more 1-arc-second mirror assemblies for imaging, spectroscopic, timing, and survey studies.

  8. Affordable and Lightweight High-Resolution X-ray Optics for Astronomical Missions

    NASA Technical Reports Server (NTRS)

    Zhang, W. W.; Biskach, M. P.; Bly, V. T.; Carter, J. M.; Chan, K. W.; Gaskin, J. A.; Hong, M.; Hohl, B. R.; Jones, W. D.; Kolodziejczak, J. J.

    2014-01-01

    Future x-ray astronomical missions require x-ray mirror assemblies that provide both high angular resolution and large photon collecting area. In addition, as x-ray astronomy undertakes more sensitive sky surveys, a large field of view is becoming increasingly important as well. Since implementation of these requirements must be carried out in broad political and economical contexts, any technology that meets these performance requirements must also be financially affordable and can be implemented on a reasonable schedule. In this paper we report on progress of an x-ray optics development program that has been designed to address all of these requirements. The program adopts the segmented optical design, thereby is capable of making both small and large mirror assemblies for missions of any size. This program has five technical elements: (1) fabrication of mirror substrates, (2) coating, (3) alignment, (4) bonding, and (5) mirror module systems engineering and testing. In the past year we have made progress in each of these five areas, advancing the angular resolution of mirror modules from 10.8 arc-seconds half-power diameter reported (HPD) a year ago to 8.3 arc-seconds now. These mirror modules have been subjected to and passed all environmental tests, including vibration, acoustic, and thermal vacuum. As such this technology is ready for implementing a mission that requires a 10-arc-second mirror assembly. Further development in the next two years would make it ready for a mission requiring a 5-arc-second mirror assembly. We expect that, by the end of this decade, this technology would enable the x-ray astrophysical community to compete effectively for a major x-ray mission in the 2020s that would require one or more 1-arc-second mirror assemblies for imaging, spectroscopic, timing, and survey studies.

  9. High resolution scanning optical imaging of a frozen polymer p-n junction

    NASA Astrophysics Data System (ADS)

    AlTal, Faleh; Gao, Jun

    2016-09-01

    Semiconductor homojunctions such as p-n or p-i-n junctions are the building blocks of many semiconductor devices such as diodes, photodetectors, transistors, or solar cells. The determination of junction depletion width is crucial for the design and realization of high-performance devices. The polymer analogue of a conventional p-n or p-i-n junction can be created by in situ electrochemical doping in a polymer light-emitting electrochemical cell (LEC). As a result of doping and junction formation, the LECs possess some highly desirable device characteristics. The LEC junction, however, is still poorly understood due to the difficulties of characterizing a dynamic-junction device. Here, we report concerted optical-beam-induced-current (OBIC) and scanning photoluminescence (PL) imaging studies of planar LECs that have been frozen to preserve the doping profile. By optimizing the cell composition, the electrode work function, and the turn-on conditions, we realize a long, straight, and highly emissive p-n junction with an interelectrode spacing of 700 μm. The extremely broad planar cell allows for time-lapse fluorescence imaging of the in situ electrochemical doping process and detailed scanning of the entire cell. A total of eighteen scans at seven locations along the junction have been performed using a versatile, custom cryogenic laser scanning apparatus. The Gaussian OBIC profiles yield an average 1/e2 junction width of only 1.5 μm, which is the smallest ever reported in a planar LEC. The controlled dedoping of the frozen device via warming cycles leads to an unexpectedly narrower OBIC profile, suggesting the presence and disappearance of fine structures at the edges of the frozen p-n junction. The results reported in this work provide new insight into the nature and structure of the LEC p-n junction. Since only about 0.2% of the entire device area is photoactive in response to an incident optical beam, the effective junction width (or volume) must be

  10. A Novel Method of Aircraft Detection Based on High-Resolution Panchromatic Optical Remote Sensing Images

    PubMed Central

    Wang, Wensheng; Nie, Ting; Fu, Tianjiao; Ren, Jianyue; Jin, Longxu

    2017-01-01

    In target detection of optical remote sensing images, two main obstacles for aircraft target detection are how to extract the candidates in complex gray-scale-multi background and how to confirm the targets in case the target shapes are deformed, irregular or asymmetric, such as that caused by natural conditions (low signal-to-noise ratio, illumination condition or swaying photographing) and occlusion by surrounding objects (boarding bridge, equipment). To solve these issues, an improved active contours algorithm, namely region-scalable fitting energy based threshold (TRSF), and a corner-convex hull based segmentation algorithm (CCHS) are proposed in this paper. Firstly, the maximal variance between-cluster algorithm (Otsu’s algorithm) and region-scalable fitting energy (RSF) algorithm are combined to solve the difficulty of targets extraction in complex and gray-scale-multi backgrounds. Secondly, based on inherent shapes and prominent corners, aircrafts are divided into five fragments by utilizing convex hulls and Harris corner points. Furthermore, a series of new structure features, which describe the proportion of targets part in the fragment to the whole fragment and the proportion of fragment to the whole hull, are identified to judge whether the targets are true or not. Experimental results show that TRSF algorithm could improve extraction accuracy in complex background, and that it is faster than some traditional active contours algorithms. The CCHS is effective to suppress the detection difficulties caused by the irregular shape. PMID:28481260

  11. High resolution mechano-optical method for acoustic field measurements in air

    NASA Astrophysics Data System (ADS)

    Welter, J. T.; Sathish, S.; Cherry, M. R.; Brodrick, P. G.

    2013-01-01

    Acoustic fields are typically visualized by measuring spatial variation of pressure in a medium, using optical (ie: Schlieren, laser interferometry) and electro-mechanical (ie: transducers, micro-electro-mechanical sensors) methods. These methods have limited ability to visualize acoustic fields in air, especially at high spatial resolution (< 0.5 mm). This paper presents a method to detect and quantify the acoustic fields in air by measuring the displacements of a micro-reflector attached to fiber with a laser interferometer. The potential of the method is demonstrated by measuring acoustic pressure of an air coupled transducer, and the variation of acoustic pressure in the focal region of an air coupled acoustic lens. In the current experimental arrangement an approximate spatial resolution of 250 microns and an approximate acoustic pressure of 7 mPa have been demonstrated. A physics based mathematical model is presented that has been used to analyze the spatial resolution and acoustic pressure. Limitations of the method and possible improvements to achieve higher spatial and temporal resolution are discussed.

  12. Mapping of photoreceptor dysfunction using high resolution three-dimensional spectral optical coherence tomography

    NASA Astrophysics Data System (ADS)

    Sikorski, B. L.; Szkulmowski, M.; Kałużny, J. J.; Bajraszewski, T.; Kowalczyk, A.; Wojtkowski, M.

    2008-02-01

    The ability to obtain reliable information on functional status of photoreceptor layer is essential for assessing vision impairment in patients with macular diseases. The reconstruction of three-dimensional retinal structure in vivo using Spectral Optical Coherence Tomography (Spectral OCT) became possible with a recent progress of the OCT field. Three-dimensional data collected by Spectral OCT devices comprise information on light intensity back-reflected from the junction between photoreceptor outer and inner segments (IS/OS) and thus can be used for evaluating photoreceptors impairment. In this paper, we introduced so called Spectral OCT reflectivity maps - a new method of selecting and displaying the spatial distribution of reflectivity of individual retinal layers. We analyzed the reflectivity of the IS/OS layer in various macular diseases. We have measured eyes of 49 patients with photoreceptor dysfunction in course of age-related macular degeneration, macular holes, central serous chorioretinopathy, acute zonal occult outer retinopathy, multiple evanescent white dot syndrome, acute posterior multifocal placoid pigment epitheliopathy, drug-induced retinopathy and congenital disorders.

  13. High-resolution contrast-enhanced optical coherence tomography in mice retinae

    NASA Astrophysics Data System (ADS)

    Sen, Debasish; SoRelle, Elliott D.; Liba, Orly; Dalal, Roopa; Paulus, Yannis M.; Kim, Tae-Wan; Moshfeghi, Darius M.; de la Zerda, Adam

    2016-06-01

    Optical coherence tomography (OCT) is a noninvasive interferometric imaging modality providing anatomical information at depths of millimeters and a resolution of micrometers. Conventional OCT images limit our knowledge to anatomical structures alone, without any contrast enhancement. Therefore, here we have, for the first time, optimized an OCT-based contrast-enhanced imaging system for imaging single cells and blood vessels in vivo inside the living mouse retina at subnanomolar sensitivity. We used bioconjugated gold nanorods (GNRs) as exogenous OCT contrast agents. Specifically, we used anti-mouse CD45 coated GNRs to label mouse leukocytes and mPEG-coated GNRs to determine sensitivity of GNR detection in vivo inside mice retinae. We corroborated OCT observations with hyperspectral dark-field microscopy of formalin-fixed histological sections. Our results show that mouse leukocytes that otherwise do not produce OCT contrast can be labeled with GNRs leading to significant OCT intensity equivalent to a 0.5 nM GNR solution. Furthermore, GNRs injected intravenously can be detected inside retinal blood vessels at a sensitivity of ˜0.5 nM, and GNR-labeled cells injected intravenously can be detected inside retinal capillaries by enhanced OCT contrast. We envision the unprecedented resolution and sensitivity of functionalized GNRs coupled with OCT to be adopted for longitudinal studies of retinal disorders.

  14. High-Resolution Optical Coherence Tomography Retinal Imaging: A Case Series Illustrating Potential and Limitations

    PubMed Central

    Puzyeyeva, Olena; Lam, Wai Ching; Flanagan, John G.; Brent, Michael H.; Devenyi, Robert G.; Mandelcorn, Mark S.; Wong, Tien; Hudson, Christopher

    2011-01-01

    Purpose. To present a series of retinal disease cases that were imaged by spectral domain optical coherence tomography (SD-OCT) in order to illustrate the potential and limitations of this new imaging modality. Methods. The series comprised four selected cases (one case each) of age-related macular degeneration (ARMD), diabetic retinopathy (DR), central retinal artery occlusion (CRAO), and branch retinal vein occlusion (BRVO). Patients were imaged using the Heidelberg Spectralis (Heidelberg Engineering, Germany) in SD-OCT mode. Patients also underwent digital fundus photography and clinical assessment. Results. SD-OCT imaging of a case of age-related macular degeneration revealed a subfoveal choroidal neovascular membrane with detachment of the retinal pigment epithelium (RPE) and neurosensory retina. Using SD-OCT, the cases of DR and BRVO both exhibited macular edema with cystoid spaces visible in the outer retina. Conclusions. The ability of SD-OCT to clearly and objectively elucidate subtle morphological changes within the retinal layers provides information that can be used to formulate diagnoses with greater confidence. PMID:21969910

  15. High-resolution imaging diagnosis of human fetal membrane by three-dimensional optical coherence tomography

    NASA Astrophysics Data System (ADS)

    Ren, Hugang; Avila, Cecilia; Kaplan, Cynthia; Pan, Yingtian

    2011-11-01

    Microscopic chorionic pseudocyst (MCP) arising in the chorion leave of the human fetal membrane (FM) is a clinical precursor for preeclampsia which may progress to fatal medical conditions (e.g., abortion) if left untreated. To examine the utility of three-dimensional (3D) optical coherence tomography (OCT) for noninvasive delineation of the morphology of human fetal membranes and early clinical detection of MCP, 60 human FM specimens were acquired from 10 different subjects undergoing term cesarean delivery for an ex vivo feasibility study. Our results showed that OCT was able to identify the four-layer architectures of human FMs consisting of high-scattering decidua vera (DV, average thickness dDV ~ 92+/-38 μm), low-scattering chorion and trophoblast (CT, dCT ~ 150+/-67 μm), high-scattering subepithelial amnion (A, dA ~ 95+/-36 μm), and low-scattering epithelium (E, dE ~ 29+/-8 μm). Importantly, 3D OCT was able to instantaneously detect MCPs (low scattering due to edema, fluid buildup, vasodilatation) and track (staging) their thicknesses dMCP ranging from 24 to 615 μm. It was also shown that high-frequency ultrasound was able to compliment OCT for detecting more advanced thicker MCPs (e.g., dMCP>615 μm) because of its increased imaging depth.

  16. In vivo imaging of raptor retina with ultra high resolution spectral domain optical coherence tomography

    NASA Astrophysics Data System (ADS)

    Ruggeri, Marco; Major, James C., Jr.; McKeown, Craig; Wehbe, Hassan; Jiao, Shuliang; Puliafito, Carmen A.

    2008-02-01

    Among birds, raptors are well known for their exceptional eyesight, which is partly due to the unique structure of their retina. Because the raptor retina is the most advanced of any animal species, in vivo examination of its structure would be remarkable. Furthermore, a noticeable percentage of traumatic ocular injuries are identified in birds of prey presented to rehabilitation facilities. Injuries affecting the posterior segment have been considered as a major impact on raptor vision. Hence, in vivo examination of the structure of the posterior segment of the raptors would be helpful for the diagnosis of traumatized birds. The purpose of this study is to demonstrate the application of ultrahigh-resolution Spectral Domain Optical Coherence Tomography (SD-OCT) for non contact in vivo imaging of the retina of birds of prey, which to the best of our knowledge has never been attempted. For the first time we present high quality OCT images of the retina of two species of bird of prey, one diurnal hawk and one nocturnal owl.

  17. Three Dimensional Motion Tracking for High Resolution Optical Microscopy, in vivo

    PubMed Central

    Pursley, Randall; Pohida, Thomas J.; Glancy, Brian; Taylor, Joni; Chess, David; Kellman, Peter; Xue, Hui; Balaban, Robert S.

    2013-01-01

    When conducting optical imaging experiments, in vivo, the signal to noise ratio and effective spatial and temporal resolution is fundamentally limited by physiological motion of the tissue. A 3-dimensional motion tracking scheme, using a multi-photon excitation microscope with a resonant galvanometer, (512×512 pixels at 33 frames/sec) is described to overcome physiological motion, in vivo. The use of commercially available graphical processing units permitted the rapid 3-dimensional cross-correlation of sequential volumes to detect displacements and adjust tissue position to track motions in near real-time. Motion phantom tests maintained micron resolution with displacement velocities of up to 200 μm/min, well within the drift observed in many biological tissues under physiologically relevant conditions. In vivo experiments on mouse skeletal muscle using the capillary vasculature with luminal dye as a displacement reference revealed an effective and robust method of tracking tissue motion to enable (1) signal averaging over time without compromising resolution, and (2) tracking of cellular regions during a physiological perturbation. PMID:22582797

  18. High-resolution optical coherence tomography in mouse models of genetic and induced retinal degeneration

    NASA Astrophysics Data System (ADS)

    Cimalla, Peter; Carido, Madalena; Pran Babu, Sheik; Santos-Ferreira, Tiago; Gaertner, Maria; Kordowich, Simon; Wittig, Dierk; Ader, Marius; Karl, Mike; Koch, Edmund

    2013-06-01

    For the study of disease mechanisms and the development of novel therapeutic strategies for retinal pathologies in human, rodent models play an important role. Nowadays, optical coherence tomography (OCT) allows three-dimensional investigation of retinal events over time. However, a detailed analysis of how different retinal degenerations are reflected in OCT images is still lacking in the biomedical field. Therefore, we use OCT to visualize retinal degeneration in specific mouse models in order to study disease progression in vivo and improve image interpretation of this noninvasive modality. We use a self-developed spectral domain OCT system for simultaneous dual-band imaging in the 0.8 μm- and 1.3 μm-wavelength range - the two most common spectral bands in biomedical OCT. A fiber-coupled ophthalmic scanning unit allows flexible imaging of the eye with a high axial resolution of 3 - 4 μm in tissue. Four different mouse models consisting of one genetic (rhodopsin-deficient and three induced retinal degenerations (sodium iodate-induced damage, light-induced photoreceptor damage and Kainate neurotoxin damage) were investigated. OCT imaging was performed daily or weekly, depending on the specific degeneration model, over a time period of up to 9 weeks. Individual retinal layers that were affected by the specific degeneration could successfully be identified and monitored over the observation time period. Therefore, longitudinal OCT studies deliver reliable information about the retinal microstructure and the time course of retinal degeneration processes in vivo.

  19. High Resolution Optical Spectroscopy of Rosetta Target 67P/Churyumov-Gerasimenko Using Keck HIRES

    NASA Astrophysics Data System (ADS)

    McKay, Adam; Cochran, Anita L.; Bodewits, Dennis; A'Hearn, Michael F.; Altwegg, Kathrin; Gulkis, Samuel; Snodgrass, Colin; de Val-Borro, Miguel; Kelley, Michael S.; Feaga, Lori M.; Wooden, Diane H.; Bauer, James M.; Kramer, Emily A.

    2016-10-01

    We present high spectral resolution optical spectroscopy of Rosetta target 67P/Churyumov-Gerasimenko obtained on UT Dec 26 and 27, 2015 using the HIRES instrument on Keck I when the comet was at a heliocentric distance of approximately 2 AU post-perihelion. The spectra cover a spectral range of 3500-10000 Angstroms at a spectral resolution of 67,000. These observations aim to provide high spectral resolution, large projected field of view context for the high spatial resolution and small projected field of view observations obtained from the Rosetta instrument suite. We report detections of CN, NH2, and [OI] emission. From the [OI]6300 emission we derive a water production rate of approximately 2 x 1027 mol/s. Production rates (or upper limits) for other species will be presented and placed in context with recent results from Rosetta. We will also present results pertaining to the [OI]5577 line, which combined with the [OI]6300 emission can be used as a proxy for CO2. We will compare our results to observations obtained by Rosetta as well as NEOWISE and Spitzer.

  20. High-reflectivity High-resolution X-ray Crystal Optics with Diamonds

    SciTech Connect

    Shvyd’ko, Y.; Stoupin, S; Cunsolo, A; Said, A; Huang, X

    2010-01-01

    Owing to the depth to which hard X-rays penetrate into most materials, it is commonly accepted that the only way to realize hard-X-ray mirrors with near 100% reflectance is under conditions of total external reflection at grazing incidence to a surface. At angles away from grazing incidence, substantial reflectance of hard X-rays occurs only as a result of constructive interference of the waves scattered from periodically ordered atomic planes in crystals (Bragg diffraction). Theory predicts that even at normal incidence the reflection of X-rays from diamond under the Bragg condition should approach 100% - substantially higher than from any other crystal. Here we demonstrate that commercially produced synthetic diamond crystals do indeed show an unprecedented reflecting power at normal incidence and millielectronvolt-narrow reflection bandwidths for hard X-rays. Bragg diffraction measurements of reflectivity and the energy bandwidth show remarkable agreement with theory. Such properties are valuable to the development of hard-X-ray optics, and could greatly assist the realization of fully coherent X-ray sources, such as X-ray free-electron laser oscillators.

  1. An endoscopic diffuse optical tomographic method with high resolution based on the improved FOCUSS method

    NASA Astrophysics Data System (ADS)

    Qin, Zhuanping; Ma, Wenjuan; Ren, Shuyan; Geng, Liqing; Li, Jing; Yang, Ying; Qin, Yingmei

    2017-02-01

    Endoscopic DOT has the potential to apply to cancer-related imaging in tubular organs. Although the DOT has relatively large tissue penetration depth, the endoscopic DOT is limited by the narrow space of the internal tubular tissue, so as to the relatively small penetration depth. Because some adenocarcinomas including cervical adenocarcinoma are located in deep canal, it is necessary to improve the imaging resolution under the limited measurement condition. To improve the resolution, a new FOCUSS algorithm along with the image reconstruction algorithm based on the effective detection range (EDR) is developed. This algorithm is based on the region of interest (ROI) to reduce the dimensions of the matrix. The shrinking method cuts down the computation burden. To reduce the computational complexity, double conjugate gradient method is used in the matrix inversion. For a typical inner size and optical properties of the cervix-like tubular tissue, reconstructed images from the simulation data demonstrate that the proposed method achieves equivalent image quality to that obtained from the method based on EDR when the target is close the inner boundary of the model, and with higher spatial resolution and quantitative ratio when the targets are far from the inner boundary of the model. The quantitative ratio of reconstructed absorption and reduced scattering coefficient can be up to 70% and 80% under 5mm depth, respectively. Furthermore, the two close targets with different depths can be separated from each other. The proposed method will be useful to the development of endoscopic DOT technologies in tubular organs.

  2. High-resolution contrast-enhanced optical coherence tomography in mice retinae

    PubMed Central

    Sen, Debasish; SoRelle, Elliott D.; Liba, Orly; Dalal, Roopa; Paulus, Yannis M.; Kim, Tae-Wan; Moshfeghi, Darius M.; de la Zerda, Adam

    2016-01-01

    Abstract. Optical coherence tomography (OCT) is a noninvasive interferometric imaging modality providing anatomical information at depths of millimeters and a resolution of micrometers. Conventional OCT images limit our knowledge to anatomical structures alone, without any contrast enhancement. Therefore, here we have, for the first time, optimized an OCT-based contrast-enhanced imaging system for imaging single cells and blood vessels in vivo inside the living mouse retina at subnanomolar sensitivity. We used bioconjugated gold nanorods (GNRs) as exogenous OCT contrast agents. Specifically, we used anti-mouse CD45 coated GNRs to label mouse leukocytes and mPEG-coated GNRs to determine sensitivity of GNR detection in vivo inside mice retinae. We corroborated OCT observations with hyperspectral dark-field microscopy of formalin-fixed histological sections. Our results show that mouse leukocytes that otherwise do not produce OCT contrast can be labeled with GNRs leading to significant OCT intensity equivalent to a 0.5 nM GNR solution. Furthermore, GNRs injected intravenously can be detected inside retinal blood vessels at a sensitivity of ∼0.5  nM, and GNR-labeled cells injected intravenously can be detected inside retinal capillaries by enhanced OCT contrast. We envision the unprecedented resolution and sensitivity of functionalized GNRs coupled with OCT to be adopted for longitudinal studies of retinal disorders. PMID:27264492

  3. Three-dimensional motion tracking for high-resolution optical microscopy, in vivo.

    PubMed

    Bakalar, M; Schroeder, J L; Pursley, R; Pohida, T J; Glancy, B; Taylor, J; Chess, D; Kellman, P; Xue, H; Balaban, R S

    2012-06-01

    When conducting optical imaging experiments, in vivo, the signal to noise ratio and effective spatial and temporal resolution is fundamentally limited by physiological motion of the tissue. A three-dimensional (3D) motion tracking scheme, using a multiphoton excitation microscope with a resonant galvanometer, (512 × 512 pixels at 33 frames s(-1)) is described to overcome physiological motion, in vivo. The use of commercially available graphical processing units permitted the rapid 3D cross-correlation of sequential volumes to detect displacements and adjust tissue position to track motions in near real-time. Motion phantom tests maintained micron resolution with displacement velocities of up to 200 μm min(-1), well within the drift observed in many biological tissues under physiologically relevant conditions. In vivo experiments on mouse skeletal muscle using the capillary vasculature with luminal dye as a displacement reference revealed an effective and robust method of tracking tissue motion to enable (1) signal averaging over time without compromising resolution, and (2) tracking of cellular regions during a physiological perturbation.

  4. High Resolution Imaging of Polymers Using Stochastic Optical Reconstruction Microscopy (STORM)

    NASA Astrophysics Data System (ADS)

    Gramlich, M. W.; Bae, J.; Hayward, R.; Ross, J. L.

    2013-03-01

    Recent super-resolution fluorescence imaging techniques represent attractive new methods for structural characterization of polymeric systems. STORM is a technique developed over the last decade to image structure and dynamics in biological systems. The high spatial resolution approaches that of other well-established techniques, such as atomic force microscopy (AFM) or scanning electron microscopy, but with all the advantages of a far-field optical technique. We have adapted STORM imaging techniques to polymeric materials, specifically using thin film blends of polystyrene (PS) and poly(methyl methacrylate) (PMMA) as a model system. We labeled PMMA with Alexa-647 fluorescent dye, and combined 10wt% label to un-labeled PMMA, then prepared 50:50 by weight blends with PS. We find the lateral PMMA domain size increases with film thickness. Furthermore, we show that the structure and size of the domains is equivalent to results from AFM. Funding is acknowledged from NSF MRI grant#DBI-0923318 to Ross and Wadsworth, ``Development of FPALM-STORM for Live Cell Single Molecule Microscopy'' NSF MRSEC grant #DMR-0820506 to UMass. We would like to acknowledge Rachel Letteri, Brent Hammer, Todd Emrick, Weiyin Gu, and Tom Russell for help with material preparation.

  5. High-resolution wind speed measurements using actively heated fiber optics

    NASA Astrophysics Data System (ADS)

    Sayde, Chadi; Thomas, Christoph K.; Wagner, James; Selker, John

    2015-11-01

    We present a novel technique to simultaneously measure wind speed (U) at thousands of locations continuously in time based on measurement of velocity-dependent heat transfer from a heated surface. Measuring temperature differences between paired passive and actively heated fiber-optic (AHFO) cables with a distributed temperature sensing system allowed estimation of U at over 2000 sections along the 230 m transect (resolution of 0.375 m and 5.5 s). The underlying concept is similar to that of a hot wire anemometer extended in space. The correlation coefficient between U measured by two colocated sonic anemometers and the AHFO were 0.91 during the day and 0.87 at night. The combination of classical passive and novel AHFO provides unprecedented dynamic observations of both air temperature and wind speed spanning 4 orders of magnitude in spatial scale (0.1-1000 m) while resolving individual turbulent motions, opening new opportunities for testing basic theories for near-surface geophysical flows.

  6. Dual-modal three-dimensional imaging of single cells with isometric high resolution using an optical projection tomography microscope

    NASA Astrophysics Data System (ADS)

    Miao, Qin; Rahn, J. Richard; Tourovskaia, Anna; Meyer, Michael G.; Neumann, Thomas; Nelson, Alan C.; Seibel, Eric J.

    2009-11-01

    The practice of clinical cytology relies on bright-field microscopy using absorption dyes like hematoxylin and eosin in the transmission mode, while the practice of research microscopy relies on fluorescence microscopy in the epi-illumination mode. The optical projection tomography microscope is an optical microscope that can generate 3-D images of single cells with isometric high resolution both in absorption and fluorescence mode. Although the depth of field of the microscope objective is in the submicron range, it can be extended by scanning the objective's focal plane. The extended depth of field image is similar to a projection in a conventional x-ray computed tomography. Cells suspended in optical gel flow through a custom-designed microcapillary. Multiple pseudoprojection images are taken by rotating the microcapillary. After these pseudoprojection images are further aligned, computed tomography methods are applied to create 3-D reconstruction. 3-D reconstructed images of single cells are shown in both absorption and fluorescence mode. Fluorescence spatial resolution is measured at 0.35 μm in both axial and lateral dimensions. Since fluorescence and absorption images are taken in two different rotations, mechanical error may cause misalignment of 3-D images. This mechanical error is estimated to be within the resolution of the system.

  7. An Alternative Approach for Registration of High-Resolution Satellite Optical Imagery and ICESat Laser Altimetry Data

    PubMed Central

    Liu, Shijie; Lv, Yi; Tong, Xiaohua; Xie, Huan; Liu, Jun; Chen, Lei

    2016-01-01

    Satellite optical images and altimetry data are two major data sources used in Antarctic research. The integration use of these two datasets is expected to provide more accurate and higher quality products, during which data registration is the first issue that needs to be solved. This paper presents an alternative approach for the registration of high-resolution satellite optical images and ICESat (Ice, Cloud, and land Elevation Satellite) laser altimetry data. Due to the sparse distribution characteristic of the ICESat laser point data, it is difficult and even impossible to find same-type conjugate features between ICESat data and satellite optical images. The method is implemented in a direct way to correct the point-to-line inconsistency in image space through 2D transformation between the projected terrain feature points and the corresponding 2D image lines, which is simpler than discrepancy correction in object space that requires stereo images for 3D model construction, and easier than the indirect way of image orientation correction via photogrammetric bundle adjustment. The correction parameters are further incorporated into imaging model through RPCs (Rational Polynomial Coefficients) generation/regeneration for the convenience of photogrammetric applications. The experimental results by using the ASTER (Advanced Spaceborne Thermal Emission and Reflection Radiometer) images and ZY-3 (Ziyuan-3 satellite) images for registration with ICESat data showed that sub-pixel level registration accuracies were achieved after registration, which have validated the feasibility and effectiveness of the presented approach. PMID:27898048

  8. An Alternative Approach for Registration of High-Resolution Satellite Optical Imagery and ICESat Laser Altimetry Data.

    PubMed

    Liu, Shijie; Lv, Yi; Tong, Xiaohua; Xie, Huan; Liu, Jun; Chen, Lei

    2016-11-27

    Satellite optical images and altimetry data are two major data sources used in Antarctic research. The integration use of these two datasets is expected to provide more accurate and higher quality products, during which data registration is the first issue that needs to be solved. This paper presents an alternative approach for the registration of high-resolution satellite optical images and ICESat (Ice, Cloud, and land Elevation Satellite) laser altimetry data. Due to the sparse distribution characteristic of the ICESat laser point data, it is difficult and even impossible to find same-type conjugate features between ICESat data and satellite optical images. The method is implemented in a direct way to correct the point-to-line inconsistency in image space through 2D transformation between the projected terrain feature points and the corresponding 2D image lines, which is simpler than discrepancy correction in object space that requires stereo images for 3D model construction, and easier than the indirect way of image orientation correction via photogrammetric bundle adjustment. The correction parameters are further incorporated into imaging model through RPCs (Rational Polynomial Coefficients) generation/regeneration for the convenience of photogrammetric applications. The experimental results by using the ASTER (Advanced Spaceborne Thermal Emission and Reflection Radiometer) images and ZY-3 (Ziyuan-3 satellite) images for registration with ICESat data showed that sub-pixel level registration accuracies were achieved after registration, which have validated the feasibility and effectiveness of the presented approach.

  9. High-resolution anatomic, diffusion tensor, and magnetization transfer magnetic resonance imaging of the optic chiasm at 3T.

    PubMed

    Vinogradov, Elena; Degenhardt, Alexandra; Smith, Derek; Marquis, Robert; Vartanian, Timothy K; Kinkel, Philip; Maier, Stephan E; Hackney, David B; Lenkinski, Robert E

    2005-08-01

    To evaluate techniques for anatomical and physiological imaging of the intracranial optic nerve (ON), optic chiasm (OC), and optic tract (OT) at 3T with the aim of visualizing axonal damage in multiple sclerosis (MS). Imaging was performed on a 3T scanner employing a custom-designed head coil that consisted of a coil array with four coils (30 x 30 cm(2)). Oblique fast spin echo (FSE) images, magnetization transfer (MT)-enhanced 3D gradient-echo (GRE) time-of-flight (TOF) images, and line scan diffusion images (LSDI) were obtained. Full diffusion tensor (DT) analysis was performed, and apparent diffusion coefficient (ADC), fractional anisotropy (FA), and fiber direction maps were obtained. FSE anatomic images were obtained with an in-plane resolution of 0.39 x 0.52 mm(2). The in-plane resolution of the MT and LSDI images was 0.78 x 0.78 mm(2). The OC, intracranial ON, and OT can be seen on these images. The dominant fiber orientations in the OC, ON, and OT, as derived from the DT images, are displayed. This study shows that by using 3T and a custom-designed, four-channel head coil, it is possible to acquire high-resolution anatomical and physiological images of the OC, ON, and OT. The pilot results presented here pave the way for imaging the anterior visual pathway in patients with MS. (c) 2005 Wiley-Liss, Inc.

  10. Sentinel-2 Optical High Resolution Mission for GMES Land Operational Services

    NASA Astrophysics Data System (ADS)

    Isola, Claudia; Drusch, Matthias; Gascon, Ferran; Martimort, Philippe; Del Bello, Umberto; Spoto, Francois; Sy, Omas; Laberinti, Paolo

    2010-05-01

    Long-term availability of Earth observation-based services and continuity of consistent high quality data is - apart from meteorological services - not guaranteed in Europe. In order to contribute to improve its response to ever growing challenges of global safety and climate change, Europe requires an independent sustained and reliable Earth observation system. The Global Monitoring for Environment and Security (GMES) is a European programme for the implementation of a European capacity to provide independent and permanent access to reliable Earth observation data. To ensure the operational provision of appropriate Earth-observation data the GMES Space Component (GSC) includes a series of five space missions called 'Sentinels', which are being developed by ESA specifically for GMES. The European Space Agency (ESA) in partnership with the European Commission (EC) is developing the Sentinel-2 optical imaging mission devoted to the operational monitoring of land and coastal areas. The Sentinel-2 mission is based on a twin satellites configuration deployed in polar sun-synchronous orbit and designed to offer a unique combination of systematic global coverage, high revisit (five days at equator with two satellites) and high spatial resolution imagery (10/20/60m). The Multi-Spectral Imager (MSI) features 13 spectral bands, going from visible to short wave infrared domains. The instrument is designed to provide in orbit calibration, excellent radiometric and geometric performance, and with a capability to support accurate image geo-location and co-registration. The Sentinel-2 mission is more particularly tailored to the monitoring of land terrains, including vegetation and urban areas. Sentinel-2 will ensure data continuity with the SPOT and Landsat multi-spectral sensors, while accounting for future service evolution. The lifetime of each Sentinel-2 spacecraft is specified as 7 years and propellant is sized for 12 years, including provision for de-orbiting manoeuvres at

  11. Survey of Close-in Super-Earths Using a New Generation Optical High Resolution Spectrograph

    NASA Astrophysics Data System (ADS)

    Sithajan, Sirinrat; Ge, J.; Muterspaugh, M. W.; Varosi, F.; Li, R.; Ma, B.; Thomas, N. B.; Wang, J.; Barnes, R.; Maxwell, T.

    2014-01-01

    A number of planets discovered using various ground-based and space-based surveys have allowed us to begin to study their statistical properties and find clues for their formation mechanisms. However, due to the traditionally adopted survey strategy, which is to observe stars with variable observational cadences (“run and gun” style), of high precision radial velocity (RV) surveys, the resulting survey completeness is usually low, especially at the low mass regime. This prevents us from obtaining critical data needed to investigate the statistical properties and formation mechanisms of low mass planet populations. We have designed a deep and homogeneous RV survey of ~500 nearby bright FGKM dwarfs for detecting low mass planets in 2014-2017 with a totally different survey strategy and cadence. This survey will observe every selected (bright, inactive, F5V to M4V) star ~100 times randomly spread over 300 days using our new generation extremely high Doppler precision optical spectrograph, called EXtremely high Precision ExtrasolaR planet Tracker III (EXPERT-III) 1 m/s precision), at the Automatic Spectroscopic Telescope (AST), a 2-m robotic telescope at Fairborn Observatory in Arizona. The flexible queue schedule offered by this robotic telescope greatly facilitates this high precision and high cadence survey. We have demonstrated the survey performance and predicted results through simulations. They show that an unprecedented high completeness sample of close-in super-Earths can be achieved. Therefore, this survey will not only precisely measure statistical properties of the close-in super Earth population largely uncovered by the Kepler mission, but also offer a uniquely homogeneous sample to constrain various planet formation models. Furthermore, this survey has a great sensitivity to probe super-Earth planets in habitable zones around K and M dwarfs.

  12. Fine Particulate Matter Predictions Using High Resolution Aerosol Optical Depth (AOD) Retrievals

    NASA Technical Reports Server (NTRS)

    Chudnovsky, Alexandra A.; Koutrakis, Petros; Kloog, Itai; Melly, Steven; Nordio, Francesco; Lyapustin, Alexei; Wang, Jujie; Schwartz, Joel

    2014-01-01

    To date, spatial-temporal patterns of particulate matter (PM) within urban areas have primarily been examined using models. On the other hand, satellites extend spatial coverage but their spatial resolution is too coarse. In order to address this issue, here we report on spatial variability in PM levels derived from high 1 km resolution AOD product of Multi-Angle Implementation of Atmospheric Correction (MAIAC) algorithm developed for MODIS satellite. We apply day-specific calibrations of AOD data to predict PM(sub 2.5) concentrations within the New England area of the United States. To improve the accuracy of our model, land use and meteorological variables were incorporated. We used inverse probability weighting (IPW) to account for nonrandom missingness of AOD and nested regions within days to capture spatial variation. With this approach we can control for the inherent day-to-day variability in the AOD-PM(sub 2.5) relationship, which depends on time-varying parameters such as particle optical properties, vertical and diurnal concentration profiles and ground surface reflectance among others. Out-of-sample "ten-fold" cross-validation was used to quantify the accuracy of model predictions. Our results show that the model-predicted PM(sub 2.5) mass concentrations are highly correlated with the actual observations, with out-of- sample R(sub 2) of 0.89. Furthermore, our study shows that the model captures the pollution levels along highways and many urban locations thereby extending our ability to investigate the spatial patterns of urban air quality, such as examining exposures in areas with high traffic. Our results also show high accuracy within the cities of Boston and New Haven thereby indicating that MAIAC data can be used to examine intra-urban exposure contrasts in PM(sub 2.5) levels.

  13. Development, validation, and fusion of high resolution active and passive optical imagery

    NASA Astrophysics Data System (ADS)

    Bissett, W. P.; DeBra, Sharon; Kadiwala, Mubin; Kohler, David D. R.; Mobley, Curtis; Steward, Robert G.; Weidemann, Alan; Davis, Curtiss O.; Lillycrop, Jeff; Pope, Robert

    2005-05-01

    HyperSpectral Imagery (HSI) of the coastal zone often focuses on the estimation of bathymetry. However, the estimation of bathymetry requires knowledge, or the simultaneous solution, of water column Inherent Optical Properties (IOPs) and bottom reflectance. The numerical solution to the simultaneous set of equations for bathymetry, IOPs, and bottom reflectance places high demands on the spectral quality, calibration, atmospheric correction, and Signal-to-Noise (SNR) of the HSI data stream. In October of 2002, a joint FERI/NRL/NAVO/USACE HSI/LIDAR experiment was conducted off of Looe Key, FL. This experiment yielded high quality HSI data at a 2 m resolution and bathymetric LIDAR data at a 4 m resolution. The joint data set allowed for the advancement and validation of a previously generated Look-Up-Table (LUT) approach to the simultaneous retrieval of bathymetry, IOPs, and bottom type. Bathymetric differences between the two techniques were normally distributed around a 0 mean, with the exception of two peaks. One peak related to a mechanical problem in the LIDAR detector mirrors that causes errors on the edges of the LIDAR flight lines. The other significant difference occurred in a single geographic area (Hawk Channel) suggesting an incomplete IOP or bottom reflectance description in the LUT data base. In addition, benthic habitat data from NOAA"s National Ocean Service (NOS) and the Florida Wildlife Research Institute (FWRI) provided validation data for the estimation of bottom type. Preliminary analyses of the bottom type estimation suggest that the best retrievals are for seagrass bottoms. One source of the potential difficulties may be that the LUT database was generated from a more pristine location (Lee Stocking Island, Bahamas). It is expected that fusing the HSI/LIDAR data streams should reduce the errors in bottom typing and IOP estimation.

  14. Development of an optical parametric generator with pulsed dye amplification for high-resolution laser spectroscopy

    NASA Astrophysics Data System (ADS)

    Bhuiyan, A. H.; Richardson, D. R.; Naik, S. V.; Lucht, R. P.

    2009-03-01

    An injection-seeded optical parametric generator (OPG), coupled with three pulsed dye amplification (PDA) stages, was shown to produce tunable, narrow linewidth laser radiation. The OPG was composed of a pair of beta barium borate ( β-BBO) crystals and pumped by the third harmonic (355 nm) output of a seeded Nd:YAG laser. The OPG was injection-seeded at the idler wavelength (824 nm) using an external cavity diode laser (ECDL) with a mode-hop-free tuning range of 20 GHz. Using the PDA stages, the OPG output signal (624 nm) was amplified to 19 mJ/pulse, while maintaining a spectral linewidth of approximately 160 MHz at full-width-half-maximum (FWHM) which was within a factor of 2 of the Fourier limit. A system of lenses and apertures was used to minimize amplified spontaneous emission (ASE) in the PDA stages. Using the OPG/PDA system, two-photon laser-induced fluorescence measurements of atomic oxygen were performed by sum-frequency-mixing the 624-nm beam with the third harmonic output of the seeded Nd:YAG laser to generate approximately 1 mJ/pulse of ultraviolet radiation near 226 nm. Voigt line shapes were found to be in good agreement with oxygen atom spectra in atmospheric-pressure, laminar, counter-flow flames; the magnitude of Doppler and collisional broadening was approximately the same. The measured O-atom concentration profile was found to compare well with that calculated using an opposed-flow flame code.

  15. High resolution three-dimensional reconstruction of the collagenous matrix of the human optic nerve head.

    PubMed

    Winkler, Moritz; Jester, Bryan; Nien-Shy, Chyong; Massei, Salina; Minckler, Don S; Jester, James V; Brown, Donald J

    2010-02-15

    Glaucoma is the second most common cause of blindness worldwide, leading to irreversible loss of vision. Prior studies indicate that ocular pressure-induced displacement of the lamina cribrosa (LC) may be responsible for retinal ganglion cell axon damage inside the neural canal. We present a novel approach to imaging the entire lamina cribrosa and the scleral canal at high lateral and axial resolution by using a combination of array tomography and nonlinear optical imaging of serial ultrathin orthogonal sections to detect second harmonic generated (SHG) signals from collagen. The resulting images can be analyzed individually or combined to form a three-dimensional reconstruction of the lamina. Due to the specificity of SHG generated from collagen the density and distribution of collagen inside the scleral canal can be objectively quantified with a high degree of accuracy. The reconstruction shows a non-uniform distribution of collagen along both the longitudinal and orthogonal axes. Mapping the collagen density by geographic region reveals significant differences in collagen content that result in "thin spots" with low collagen density as well as areas of very high collagen content. This suggests a non-uniform mechanical stiffness across the lamina that may account for increased axon damage observed in glaucoma patients. The inferior temporal region of the ONH in particular is marked by low collagen density, which corresponds with clinical observations identifying this region as being more susceptible to damage during the onset of glaucoma. Further application of this technique will help characterize the relationship of age, race and gender on the morphology of the LC.

  16. High-resolution Record of Holocene Climate, Vegetation, and Fire from a Raised Peat Bog, Prince Edward Island, Canadian Maritimes

    NASA Astrophysics Data System (ADS)

    Peros, M. C.; Chan, K.; Ponsford, L.; Carroll, J.; Magnan, G.

    2014-12-01

    Raised peat bogs receive all precipitation and nutrients from the atmosphere and are thus widely used archives for information on past environments and climates. In this paper we provide high-resolution multi-proxy data from a raised bog from northeastern Prince Edward Island, located in the Gulf of St. Lawrence, Canada. We studied testate amoeba (a proxy for water table depth), macrocharcoal (a proxy for local-scale fire), peat humification (a proxy for decomposition), plant macrofossils (indicative of local-scale vegetation), and organic matter content (yielding carbon accumulation rates) from a 5.5 m long core lifted from the center of Baltic Bog. Eleven AMS radiocarbon dates show that peat accumulation began before 9000 cal yr BP and continued almost uninterrupted until the present. The macrofossil data show that a transition from a sedge-dominated fen to a sphagnum-dominated bog occurred around 8000 cal yr BP, and sphagnum remained dominant in the bog throughout most of the Holocene. A testate amoeba-based reconstruction of water table depth indicates that conditions were drier during the early Holocene (~8000 to 5000 cal yr BP) and became gradually wetter into the late Holocene. In addition, a number of higher frequency shifts in precipitation are inferred throughout the Holocene on the basis of the testate amoeba and humification results. The macrocharcoal evidence indicates fire—probably in the surrounding forest—was relatively more common during the early Holocene, perhaps due to drier climate conditions. A large influx of charcoal at around 2000 cal yr BP suggests the presence of one or more major fires at this time, and a concurrent decrease in the rate of peat accumulation indicates the fire may have affected the bog itself. The data from Baltic Bog is broadly comparable to other proxy data (in particular pollen studies) from the Canadian Maritimes. This work is important because it: 1) helps us better understand the role of hydroclimatic

  17. High-Resolution Recording of the Circadian Oscillator in Primary Mouse α- and β-Cell Culture.

    PubMed

    Petrenko, Volodymyr; Gosmain, Yvan; Dibner, Charna

    2017-01-01

    Circadian clocks have been developed in evolution as an anticipatory mechanism allowing for adaptation to the constantly changing light environment due to rotation of the Earth. This mechanism is functional in all light-sensitive organisms. There is a considerable body of evidence on the tight connection between the circadian clock and most aspects of physiology and metabolism. Clocks, operative in the pancreatic islets, have caught particular attention in the last years due to recent reports on their critical roles in regulation of insulin secretion and etiology of type 2 diabetes. While β-cell clocks have been extensively studied during the last years, α-cell clocks and their role in islet function and orchestration of glucose metabolism stayed unexplored, largely due to the difficulty to isolate α-cells, which represents a considerable technical challenge. Here, we provide a detailed description of an experimental approach for the isolation of separate mouse α- and β-cell population, culture of isolated primary α- and β-cells, and their subsequent long-term high-resolution circadian bioluminescence recording. For this purpose, a triple reporter ProGlucagon-Venus/RIP-Cherry/Per2:Luciferase mouse line was established, carrying specific fluorescent reporters for α- and β-cells, and luciferase reporter for monitoring the molecular clockwork. Flow cytometry fluorescence-activated cell sorting allowed separating pure α- and β-cell populations from isolated islets. Experimental conditions, developed by us for the culture of functional primary mouse α- and β-cells for at least 10 days, will be highlighted. Importantly, temporal analysis of freshly isolated α- and β-cells around-the-clock revealed preserved rhythmicity of core clock genes expression. Finally, we describe the setting to assess circadian rhythm in cultured α- and β-cells synchronized in vitro. The here-described methodology allows to analyze the functional properties of primary α- and

  18. High-Resolution Geochemical and Paleoecological Records of Climate Change Since the Late Glacial at Lake Tanganyika, East Africa

    NASA Astrophysics Data System (ADS)

    Alin, S. R.; Cohen, A. S.

    2002-12-01

    We used high-resolution geochemical and paleoecological records from shallow-water sediment cores to refine previous descriptions of climatic conditions at Lake Tanganyika, East Africa, for the period from the Late Glacial to the present. Radiocarbon and 210Pb dating were used to establish chronologies for the cores. Sedimentological changes indicate that lake level has risen approximately 50-70 m since the Late Glacial. A depositional hiatus occurred between 6.4 and 11.4 ka BP (all dates in calendar years) in several of the shallow-water cores. Elemental abundance (%C, %N) and stable isotopic (δ15N, δ13C) data for one core suggest that substantial changes in primary productivity and nutrient recycling regimes have occurred since 6.4 ka BP. Carbonate and ostracode crustacean preservation were low and nil, respectively, prior to 2.4 ka BP. Generally, these data support previous interpretations of regional paleoclimate and lake conditions, with wet and warm conditions during the interval from 6.4 to 4.0 ka, and increasingly arid conditions since 2.4 ka. However, for the interval from 4.0 to 2.4 ka, paleoenvironmental indicators (δ15N, reduced carbonate and ostracode preservation) suggest that the central part of Lake Tanganyika was stably stratified at a shallower depth than present as a result of diminished southerly trade winds. After 2.4 ka BP, sedimentary carbonate concentrations increase, and δ13C values become enriched, suggesting that lacustrine productivity increased with the resumption of deeper wind-driven mixing, lasting until 1 ka BP. For post-2.4 ka samples, species abundance data for ostracodes were used to generate an ostracode water depth index (OWDI). OWDI indicated that severe drought conditions were persistent or recurred at Lake Tanganyika between 1550 and 1850 A.D. Droughts resulted in marked lowstands at Lake Tanganyika at 1580+/-15 A.D., 1730+/-35 A.D., and 1800+/-30 A.D. These data contribute new information on the timing of Little Ice Age

  19. Chloride, nitrate and sulphate high resolution records for the last two glacial-interglacial cycles from EDC ice core.

    NASA Astrophysics Data System (ADS)

    Migliori, A.; Becagli, S.; Benassai, S.; Castellano, E.; Severi, M.; Traversi, R.; Udisti, R.

    2003-04-01

    During the 2001/2002 Antarctic campaign, in field Fast Ion Chromatographic analysis was continued on the EPICA Dome C ice core (EDC) yielding chloride, nitrate and sulphate high-resolution records up to 2160 m depth. This depth interval is supposed to cover the last two glacial/interglacial cycles. Since EDC isotopic measurements are in progress at present, chemical profiles were compared with deuterium profile from Vostok ice core, aiming to study the relationship between climatic and environmental changes. All the components show lower and rather constant background levels over the two interglacial periods (Holocene and Eemian) with respect to glacial ones. Sulphate background profile, perturbed by a large number of volcanic spikes, shows a little sensitivity to glacial/interglacial climatic changes. Since the variations of the sulphate concentration are similar in extent to the estimated snow accumulation changes, this component does not seem to be affected by significant changes in source intensity and transport efficiency during the different climatic periods. Chloride and nitrate concentration profiles exhibit a higher variability along the ice core, with very low and stable interglacial values and high and noisy glacial levels. By comparing the Vostok deuterium profile in the last glacial period, where the temporal resolution of isotopic measurement is particularly high, a good correspondence between chloride maxima and deuterium minima can be observed, even for short-term variations. Both environmental variations and glaciological features can modulate the response of chloride to the temperature changes. Indeed, post-depositional effects through HCl re-emission into the atmosphere heavily affect chloride. Its persistence in the snow layers is therefore related to snow accumulation rate and snow acidity. In cold periods, despite the lower accumulation rate, the higher alkaline dust atmospheric load is able to fix higher chloride snow concentration preventing

  20. Quantification of in vivo colonic motor patterns in healthy humans before and after a meal revealed by high-resolution fiber-optic manometry

    PubMed Central

    DINNING, P. G.; WIKLENDT, L.; MASLEN, L.; GIBBINS, I.; PATTON, V.; ARKWRIGHT, J. W.; LUBOWSKI, D. Z.; O'GRADY, G.; BAMPTON, P. A.; BROOKES, S. J.; COSTA, M.

    2015-01-01

    Background Until recently, investigations of the normal patterns of motility of the healthy human colon have been limited by the resolution of in vivo recording techniques. Methods We have used a new, high-resolution fiber-optic manometry system (72 sensors at 1-cm intervals) to record motor activity from colon in 10 healthy human subjects. Key Results In the fasted colon, on the basis of rate and extent of propagation, four types of propagating motor pattern could be identified: (i) cyclic motor patterns (at 2–6/min); (ii) short single motor patterns; (iii) long single motor patterns; and (iv) occasional retrograde, slow motor patterns. For the most part, the cyclic and short single motor patterns propagated in a retrograde direction. Following a 700 kCal meal, a fifth motor pattern appeared; high-amplitude propagating sequences (HAPS) and there was large increase in retrograde cyclic motor patterns (5.6±5.4/2 h vs 34.7±19.8/2 h; p < 0.001). The duration and amplitude of individual pressure events were significantly correlated. Discriminant and multivariate analysis of duration, gradient, and amplitude of the pressure events that made up propagating motor patterns distinguished clearly two types of pressure events: those belonging to HAPS and those belonging to all other propagating motor patterns. Conclusions & Inferences This work provides the first comprehensive description of colonic motor patterns recorded by high-resolution manometry and demonstrates an abundance of retrograde propagating motor patterns. The propagating motor patterns appear to be generated by two independent sources, potentially indicating their neurogenic or myogenic origin. PMID:25131177

  1. Using fire regimes to delineate zones in a high-resolution lake sediment record from the western United States

    Treesearch

    Jesse L. Morris; Andrea Brunelle; R. Justin DeRose; Heikki Seppa; Mitchell J. Power; Vachel Carter; Ryan Bares

    2013-01-01

    Paleoenvironmental reconstructions are important for understanding the influence of long-term climate variability on ecosystems and landscape disturbance dynamics. In this paper we explore the linkages among past climate, vegetation, and fire regimes using a high-resolution pollen and charcoal reconstruction from Morris Pond located on the Markagunt Plateau in...

  2. A high-resolution record of climate variability and landscape response from Kettle Lake, northern Great Plains, North America

    NASA Astrophysics Data System (ADS)

    Grimm, Eric C.; Donovan, Joseph J.; Brown, Kendrick J.

    2011-09-01

    A decadal-scale multiproxy record of minerals, pollen, and charcoal from Kettle Lake, North Dakota provides a high-resolution record of climate and vegetation change spanning the entire Holocene from the northern Great Plains (NGP) in North America. The chronology is established by over 50 AMS radiocarbon dates. This record exhibits millennial-scale trends evident in other lower-resolution studies, but with much more detail on short-term climate variability and on the rapidity and timing of major climatic shifts. As a proxy for precipitation, we utilize the rate of endogenic carbonate sedimentation, which depends on groundwater inflow, which in turn depends on precipitation. Independent cluster analyses of mineral and pollen data reveal major Holocene mode shifts at 10.73 ka (ka = cal yr BP), 9.25 ka, and 4.44 ka. The early Holocene, 11.7-9.25 ka, was generally wet, with perhaps a trend to higher evaporation associated with warming temperatures. A switch from calcite to aragonite deposition associated with a severe, but brief drought occurred at 10.73 ka. From 10.73 ka to 9.25 ka, climate was generally humid but punctuated at 100-300 yr intervals by brief droughts, including the most severe drought of the entire Holocene at 9.25 ka. This event was coeval with the 9.3-9.2 ka event in the Greenland ice cores and observed at a number of sites worldwide. In contrast, the prominent 8.2 ka event in Greenland is not remarkable at Kettle Lake. The prominence of the 9.25 event locally in the NGP may be due to a major drawdown and northward retreat of Lake Agassiz at this time, reducing its mesoclimatic effect on the NGP and thrusting the region into an insolation controlled regime. The mid-Holocene, 9.25-4.44 ka, was characterized by great variability in moisture on a multi-decadal scale, with severe droughts alternating with more humid periods. The high abundance of the weedy but drought intolerant Ambrosia generally during the mid-Holocene and specifically during the

  3. Next Generation X-Ray Optics: High-Resolution, Light-Weight, and Low-Cost

    NASA Technical Reports Server (NTRS)

    Zhang, William W.

    2011-01-01

    segments is the continued development and perfection of alignment and integration techniques, for incorporating individual mirror segments into a precision mirror assembly. Recently, we have been developing a technique called edge-bonding, which has achieved an accuracy to enable 10- arcsecond x-ray telescopes. Currently, we are investigating and improving the long-term alignment stability of so-bonded mirrors. Next, we shall refine this process to enable 5-arsecond x-ray telescopes. This technology development program includes all elements to demonstrate progress toward TRL-6: metrology; x-ray performance tests; coupled structural, thermal, and optical performance analysis, and environmental testing.

  4. Next Generation X-Ray Optics: High-Resolution, Light-Weight, and Low-Cost

    NASA Technical Reports Server (NTRS)

    Zhang, William W.

    2012-01-01

    mirror segments is the continued development and perfection of alignment and integration techniques, for incorporating individual mirror segments into a precision mirror assembly. Recently, we have been developing a technique called edge-bonding, which has achieved an accuracy to enable 10-arcsecond x-ray telescopes. Currently, we are investigating and improving the long-term alignment stability of so-bonded mirrors. Next, we shall refine this process to enable 5-arsecond x-ray telescopes. This technology development program includes all elements to demonstrate progress toward TRL-6: metrology; x-ray performance tests; coupled structural, thermal, and optical performance analysis, and environmental testing.

  5. High-Resolution Profiling of Richardson Number Across the Surface Boundary Layer Using Heated Fiber Optics

    NASA Astrophysics Data System (ADS)

    Sayde, C.; Higgins, C. W.; Perdosa, R.; Mahaffee, W.; Selker, J. S.

    2016-12-01

    Most critical atmospheric processes are a balance between buoyancy and shear, typically measured with the Richardson number. The fine scale motions associated with critical or near critical valued of Richardson number are understudied because the location and timing of these events are not known a-priori. To study these motions and quantify their importance for transport of heat momentum and water vapor in the atmospheric boundary layer, a distributed measurement approach for temperature and wind speed is required. Here we present the results of 12.5 cm resolution distributed profiling of wind speed and temperature for the first 37 m of the surface boundary layer. Distributed Temperature Sensing (DTS) technology was employed to measure temperature every 5 s and 12.5 cm along two Fiber Optics (FO) cables suspended from 37 m elevation to ground by a blimp anchored above a vineyard in the Willamette Valley, Oregon. 3D printed FO holders installed every 3 m along the suspended FO cables insured constant spacing of 7.5 cm between the two cables. The first FO cable was 0.9mm in diameter and reported ambient air temperature. The second FO cable was embedded in a thin stainless steel tube (1.3 mm OD) continuously heated by an electrical current to provide continuous wind speed measurements every 12.5 cm along the heated cable. Analogous to a hot-wire anemometer, this approach is based on the principal of velocity-dependent heat transfer from a heated surface. The co-located wind speed and ambient temperature measurements are used to calculate Richardson number with a spatial and temporal resolution of 12.5 cm and 5 s respectively for the first 37 m of the surface boundary layer. The equipment employed, including the heating system, which is available to all US scientists, was provided by CTEMPs.org thanks to the generous grant support from the National Science Foundation under Grant Number EAR 0930061. Any opinions, findings, and conclusions or recommendations expressed in

  6. Sentinel-2 Optical High Resolution Mission for GMES Land Operational Services

    NASA Astrophysics Data System (ADS)

    Drusch, M.; Gascon, F.; Martimort, P.; Spoto, F.

    2009-12-01

    In the framework of the Global Monitoring for Environment and Security (GMES) programme, the European Space Agency (ESA) in partnership with the European Commission (EC) is developing the Sentinel-2 optical imaging mission devoted to the operational monitoring of land and coastal areas. The Sentinel-2 mission is based on a twin satellites configuration deployed in polar sun-synchronous orbit and designed to offer a unique combination of systematic global coverage, high revisit (five days at equator with two satellites) and high spatial resolution imagery (10/20/60m). The Multispectral instrument features 13 spectral bands, going from visible to short wave infrared domains. The instrument is designed to provide in orbit calibration, excellent radiometric and geometric performance, and with a capability to support accurate image geolocation and co-registration. The Sentinel-2 mission is more particularly tailored to the monitoring of land terrains, including vegetation and urban areas. Sentinel-2 will ensure data continuity with the SPOT and Landsat multi-spectral sensors, while accounting for future service evolution. The lifetime of each Sentinel-2 spacecraft is specified as 7 years and propellant is sized for 12 years, including provision for de-orbiting manoeuvres at end-of-life. The satellite will be three-axis stabilized with an AOCS based on high-rate multi-head star trackers, mounted on the instrument structure for better pointing accuracy and stability, as well as a laser gyroscope and a dual-frequency GNSS receiver. The Multi-Spectral Instrument (MSI) is based on the pushbroom concept. It features a Three Mirror Anastigmat (TMA) telescope with a pupil diameter of about 150 mm, and achieves a very good imaging quality all across its wide Field of View (290 km swath width, significantly enlarged with respect to Landsat and SPOT). The telescope structure and the mirrors are made of silicon carbide for minimizing thermo-elastic deformations. The visible and

  7. High resolution archives from the Black Sea and Caspian Sea as records of hydrological changes during the Holocene

    NASA Astrophysics Data System (ADS)

    Marret, F.; Mudie, P.; Leroy, S.; Aksu, A.; Hiscott, R.

    2009-04-01

    Studies of multiple proxies from sediments of marginal seas, such as the Black Sea, or epicontinental seas, such as the Caspian Sea, have demonstrated their great potential to document past changes in climate and hydrological cycles. Over the last decade, the Black Sea has been the centre of focus with regards to the timing, speed and extent of its reconnection with the Mediterranean Sea at the beginning of the Holocene; this has led to controversial debate in the scientific community and world-wide public interest. Notably, it has been suggested that the speed of this catastrophic flooding event led to the dispersal of Neolithic populations living on the northern shore of the Black Sea. The evidence for this abrupt flooding hypothesis included geophysical surveys, sedimentological and geochemical studies on the western and northern shelves. Although the interpretation of these data may be equivocal, it appears that a major environmental change occurred at around 8.5 ka BP as evidenced by a number of proxies (1). The Caspian Sea has revealed significant information with regards to modern marine proxies that were previously identified in early Holocene Black Sea sediments. Their modern distribution in the Caspian Sea has allowed to better constraint the paleoconditions of the Black Sea before its reconnection with the Mediterranean Sea at the beginning of the Holocene (2). Further studies of late Holocene Caspian Sea sediments have highlighted changes in salinity due to modification in river flows and climate change (3). We present here evidence for the gradual pace of this transformation of the Black Sea, from a brackish lake to its present-day marine conditions, based on a high resolution record of dinoflagellate cysts that are used to reconstruct past sea-surface conditions. Most previous Black Sea dinocyst studies were conducted at a low resolution, on the order of millennia, whereas our new record has a multi-decadal resolution. This has enabled us to pinpoint

  8. A comparative study of noise in supercontinuum light sources for ultra-high resolution optical coherence tomography

    NASA Astrophysics Data System (ADS)

    Maria, M.; Gonzalo, I. B.; Bondu, M.; Engelsholm, R. D.; Feuchter, T.; Moselund, P. M.; Leick, L.; Bang, O.; Podoleanu, A.

    2017-03-01

    Supercontinuum (SC) light is a well-established technology, which finds applications in several domains ranging from chemistry to material science and imaging systems [1-2]. More specifically, its ultra-wide optical bandwidth and high average power make it an ideal tool for Optical Coherence Tomography (OCT). Over the last 5 years, numerous examples have demonstrated its high potential [3-4] in this context. However, SC light sources present pulse-to-pulse intensity variation that can limit the performance of any OCT system [5] by degrading their signal to noise ratio (SNR). To this goal, we have studied and compared the noise of several SC light sources and evaluated how their noise properties affect the performance of Ultra-High Resolution OCT (UHR-OCT) at 1300 nm. We have measured several SC light sources with different parameters (pulse length, en