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Sample records for highly efficient transduction

  1. Highly efficient transduction of primary adult CNS and PNS neurons

    PubMed Central

    Levin, Evgeny; Diekmann, Heike; Fischer, Dietmar

    2016-01-01

    Delivery and expression of recombinant genes, a key methodology for many applications in biological research, remains a challenge especially for mature neurons. Here, we report easy, highly efficient and well tolerated transduction of adult peripheral and central neuronal populations of diverse species in culture using VSV-G pseudo-typed, recombinant baculovirus (BacMam). Transduction rates of up to 80% were reliably achieved at high multiplicity of infection without apparent neuro-cytopathic effects. Neurons could be transduced either shortly after plating or after several days in culture. Co-incubation with two different baculoviruses attained near complete co-localization of fluorescent protein expression, indicating multigene delivery. Finally, evidence for functional protein expression is provided by means of cre-mediated genetic recombination and neurite outgrowth assays. Recombinant protein was already detected within hours after transduction, thereby enabling functional readouts even in relatively short-lived neuronal cultures. Altogether, these results substantiate the usefulness of baculovirus-mediated transduction of mature neurons for future research in neuroscience. PMID:27958330

  2. Generation of Functional Human Cardiac Progenitor Cells by High-Efficiency Protein Transduction

    PubMed Central

    Li, Xiao-Hong; Li, Qianqian; Jiang, Lin; Deng, Chunyu; Liu, Zaiyi; Fu, Yongheng; Zhang, Mengzhen; Tan, Honghong; Feng, Yuliang; Shan, Zhixin

    2015-01-01

    The reprogramming of fibroblasts to induced pluripotent stem cells raises the possibility that somatic cells could be directly reprogrammed to cardiac progenitor cells (CPCs). The present study aimed to assess highly efficient protein-based approaches to reduce or eliminate the genetic manipulations to generate CPCs for cardiac regeneration therapy. A combination of QQ-reagent-modified Gata4, Hand2, Mef2c, and Tbx5 and three cytokines rapidly and efficiently reprogrammed human dermal fibroblasts (HDFs) into CPCs. This reprogramming process enriched trimethylated histone H3 lysine 4, monoacetylated histone H3 lysine 9, and Baf60c at the Nkx2.5 cardiac enhancer region by the chromatin immunoprecipitation quantitative polymerase chain reaction assay. Protein-induced CPCs transplanted into rat hearts after myocardial infarction improved cardiac function, and this was related to differentiation into cardiomyocyte-like cells. These findings demonstrate that the highly efficient protein-transduction method can directly reprogram HDFs into CPCs. This protein reprogramming strategy lays the foundation for future refinements both in vitro and in vivo and might provide a source of CPCs for regenerative approaches. Significance The findings from the present study have demonstrated an efficient protein-transduction method of directly reprogramming fibroblasts into cardiac progenitor cells. These results have great potential in cell-based therapy for cardiovascular diseases. PMID:26564862

  3. Development of Optimized AAV Serotype Vectors for High-Efficiency Transduction at Further Reduced Doses.

    PubMed

    Ling, Chen; Li, Baozheng; Ma, Wenqin; Srivastava, Arun

    2016-08-01

    We have described the development of capsid-modified next-generation AAV vectors for both AAV2 and AAV3 serotypes, in which specific surface-exposed tyrosine (Y), serine (S), threonine (T), and lysine (K) residues on viral capsids were modified to achieve high-efficiency transduction at lower doses. We have also described the development of genome-modified AAV vectors, in which the transcriptionally inactive, single-stranded AAV genome was modified to achieve improved transgene expression. Here, we describe that combination of capsid modifications and genome modifications leads to the generation of optimized AAV serotype vectors, which transduce cells and tissues more efficiently, both in vitro and in vivo, at ∼20-30-fold reduced doses. These studies have significant implications in the potential use of the optimized AAV serotype vectors in human gene therapy.

  4. Highly efficient intracellular transduction in three-dimensional gradients for programming cell fate.

    PubMed

    Eltaher, Hoda M; Yang, Jing; Shakesheff, Kevin M; Dixon, James E

    2016-09-01

    Fundamental behaviour such as cell fate, growth and death are mediated through the control of key genetic transcriptional regulators. These regulators are activated or repressed by the integration of multiple signalling molecules in spatio-temporal gradients. Engineering these gradients is complex but considered key in controlling tissue formation in regenerative medicine approaches. Direct programming of cells using exogenously delivered transcription factors can by-pass growth factor complexity but there is still a requirement to deliver such activity spatio-temporally. We previously developed a technology termed GAG-binding enhanced transduction (GET) to efficiently deliver a variety of cargoes intracellularly using GAG-binding domains to promote cell targeting, and cell penetrating peptides (CPPs) to allow cell entry. Herein we demonstrate that GET can be used in a three dimensional (3D) hydrogel matrix to produce gradients of intracellular transduction of mammalian cells. Using a compartmentalised diffusion model with a source-gel-sink (So-G-Si) assembly, we created gradients of reporter proteins (mRFP1-tagged) and a transcription factor (TF, myogenic master regulator MyoD) and showed that GET can be used to deliver molecules into cells spatio-temporally by monitoring intracellular transduction and gene expression programming as a function of location and time. The ability to spatio-temporally control the intracellular delivery of functional proteins will allow the establishment of gradients of cell programming in hydrogels and approaches to direct cellular behaviour for many regenerative medicine applications. Regenerative medicine aims to reform functional biological tissues by controlling cell behaviour. Growth factors (GFs) are soluble cues presented to cells in spatio-temporal gradients and play important roles programming cell fate and gene expression. The efficient transduction of cells by GET (Glycosaminoglycan-enhanced transducing

  5. A Simple High Efficiency Intra-Islet Transduction Protocol Using Lentiviral Vectors.

    PubMed

    Jimenez-Moreno, Carmen Maria; Herrera-Gomez, Irene de Gracia; Lopez-Noriega, Livia; Lorenzo, Petra Isabel; Cobo-Vuilleumier, Nadia; Fuente-Martin, Esther; Mellado-Gil, Jose Manuel; Parnaud, Geraldine; Bosco, Domenico; Gauthier, Benoit Raymond; Martin-Montalvo, Alejandro

    2015-01-01

    Successful normalization of blood glucose in patients transplanted with pancreatic islets isolated from cadaveric donors established the proof-of-concept that Type 1 Diabetes Mellitus is a curable disease. Nonetheless, major caveats to the widespread use of this cell therapy approach have been the shortage of islets combined with the low viability and functional rates subsequent to transplantation. Gene therapy targeted to enhance survival and performance prior to transplantation could offer a feasible approach to circumvent these issues and sustain a durable functional β-cell mass in vivo. However, efficient and safe delivery of nucleic acids to intact islet remains a challenging task. Here we describe a simple and easy-to-use lentiviral transduction protocol that allows the transduction of approximately 80 % of mouse and human islet cells while preserving islet architecture, metabolic function and glucose-dependent stimulation of insulin secretion. Our protocol will facilitate to fully determine the potential of gene expression modulation of therapeutically promising targets in entire pancreatic islets for xenotransplantation purposes.

  6. Controlled release of GAG-binding enhanced transduction (GET) peptides for sustained and highly efficient intracellular delivery.

    PubMed

    Abu-Awwad, Hosam Al-Deen M; Thiagarajan, Lalitha; Dixon, James E

    2017-07-15

    Controlled release systems for therapeutic molecules are vital to allow the sustained local delivery of their activities which direct cell behaviour and enable novel regenerative strategies. Direct programming of cells using exogenously delivered transcription factors can by-pass growth factor signalling but there is still a requirement to deliver such activity spatio-temporally. We previously developed a technology termed GAG-binding enhanced transduction (GET) to efficiently deliver a variety of cargoes intracellularly, using GAG-binding domains which promote cell targeting, and cell penetrating peptides (CPPs) which allow cell entry. Herein we demonstrate that GET system can be used in controlled release systems to mediate sustained intracellular transduction over one week. We assessed the stability and activity of GET peptides in poly(dl-lactic acid-co-glycolic acid) (PLGA) microparticles (MPs) prepared using a S/O/W double emulsion method. Efficient encapsulation (∼65%) and tailored protein release profiles could be achieved, however intracellular transduction was significantly inhibited post-release. To retain GET peptide activity we optimized a strategy of co-encapsulation of l-Histidine, which may form a complex with the PLGA degradation products under acidic conditions. Simulations of the polymer microclimate showed that hydrolytic acidic PLGA degradation products directly inhibited GET peptide transduction activity, and use of l-Histidine significantly enhanced released protein delivery. The ability to control the intracellular transduction of functional proteins into cells will facilitate new localized delivery methods and allow approaches to direct cellular behaviour for many regenerative medicine applications. The goal for regenerative medicine is to restore functional biological tissue by controlling and augmenting cellular behaviour. Either Transcription (TFs) or growth factors (GFs) can be presented to cells in spatio-temporal gradients for

  7. High-efficiency transduction of fibroblasts and mesenchymal stem cells by tyrosine-mutant AAV2 vectors for their potential use in cellular therapy.

    PubMed

    Li, Mengxin; Jayandharan, Giridhara R; Li, Baozheng; Ling, Chen; Ma, Wenqin; Srivastava, Arun; Zhong, Li

    2010-11-01

    Adeno-associated virus 2 (AAV2) vectors transduce fibroblasts and mesenchymal stem cells (MSCs) inefficiently, which limits their potential widespread applicability in combinatorial gene and cell therapy. We have reported that AAV2 vectors fail to traffic efficiently to the nucleus in murine fibroblasts. We have also reported that site-directed mutagenesis of surface-exposed tyrosine residues on viral capsids leads to improved intracellular trafficking of the mutant vectors, and the transduction efficiency of the single tyrosine-mutant vectors is ∼10-fold higher in human cells. In the current studies, we evaluated the transduction efficiency of single as well as multiple tyrosine-mutant AAV2 vectors in murine fibroblasts. Our results indicate that the Y444F mutant vectors transduce these cells most efficiently among the seven single-mutant vectors, with >30-fold increase in transgene expression compared with the wild-type vectors. When the Y444F mutation is combined with additional mutations (Y500F and Y730F), the transduction efficiency of the triple-mutant vectors is increased by ∼130-fold and the viral intracellular trafficking is also significant improved. Similarly, the triple-mutant vectors are capable of transducing up to 80-90% of bone marrow-derived primary murine as well as human MSCs. Thus, high-efficiency transduction of fibroblasts with reprogramming genes to generate induced pluripotent stem cells, and the MSCs for delivering therapeutic genes, should now be feasible with the tyrosine-mutant AAV vectors.

  8. High-Efficiency Transduction of Primary Human Hematopoietic Stem/Progenitor Cells by AAV6 Vectors: Strategies for Overcoming Donor-Variation and Implications in Genome Editing

    PubMed Central

    Ling, Chen; Bhukhai, Kanit; Yin, Zifei; Tan, Mengqun; Yoder, Mervin C.; Leboulch, Philippe; Payen, Emmanuel; Srivastava, Arun

    2016-01-01

    We have reported that of the 10 commonly used AAV serotype vectors, AAV6 is the most efficient in transducing primary human hematopoietic stem/progenitor cells (HSPCs). However, the transduction efficiency of the wild-type (WT) AAV6 vector varies greatly in HSPCs from different donors. Here we report two distinct strategies to further increase the transduction efficiency in HSPCs from donors that are transduced less efficiently with the WT AAV6 vectors. The first strategy involved modifications of the viral capsid proteins where specific surface-exposed tyrosine (Y) and threonine (T) residues were mutagenized to generate a triple-mutant (Y705 + Y731F + T492V) AAV6 vector. The second strategy involved the use of ex vivo transduction at high cell density. The combined use of these strategies resulted in transduction efficiency exceeding ~90% in HSPCs at significantly reduced vector doses. Our studies have significant implications in the optimal use of capsid-optimized AAV6 vectors in genome editing in HSPCs. PMID:27759036

  9. High-Efficiency Transduction of Fibroblasts and Mesenchymal Stem Cells by Tyrosine-Mutant AAV2 Vectors for Their Potential Use in Cellular Therapy

    PubMed Central

    Li, Mengxin; Jayandharan, Giridhara R.; Li, Baozheng; Ling, Chen; Ma, Wenqin; Srivastava, Arun

    2010-01-01

    Abstract Adeno-associated virus 2 (AAV2) vectors transduce fibroblasts and mesenchymal stem cells (MSCs) inefficiently, which limits their potential widespread applicability in combinatorial gene and cell therapy. We have reported that AAV2 vectors fail to traffic efficiently to the nucleus in murine fibroblasts. We have also reported that site-directed mutagenesis of surface-exposed tyrosine residues on viral capsids leads to improved intracellular trafficking of the mutant vectors, and the transduction efficiency of the single tyrosine-mutant vectors is ∼10-fold higher in human cells. In the current studies, we evaluated the transduction efficiency of single as well as multiple tyrosine-mutant AAV2 vectors in murine fibroblasts. Our results indicate that the Y444F mutant vectors transduce these cells most efficiently among the seven single-mutant vectors, with >30-fold increase in transgene expression compared with the wild-type vectors. When the Y444F mutation is combined with additional mutations (Y500F and Y730F), the transduction efficiency of the triple-mutant vectors is increased by ∼130-fold and the viral intracellular trafficking is also significant improved. Similarly, the triple-mutant vectors are capable of transducing up to 80–90% of bone marrow-derived primary murine as well as human MSCs. Thus, high-efficiency transduction of fibroblasts with reprogramming genes to generate induced pluripotent stem cells, and the MSCs for delivering therapeutic genes, should now be feasible with the tyrosine-mutant AAV vectors. PMID:20507237

  10. Highly efficient tumor transduction and antitumor efficacy in experimental human malignant mesothelioma using replicating gibbon ape leukemia virus.

    PubMed

    Kubo, S; Takagi-Kimura, M; Logg, C R; Kasahara, N

    2013-12-01

    Retroviral replicating vectors (RRVs) have been shown to achieve efficient tumor transduction and enhanced therapeutic benefit in a wide variety of cancer models. Here we evaluated two different RRVs derived from amphotropic murine leukemia virus (AMLV) and gibbon ape leukemia virus (GALV), in human malignant mesothelioma cells. In vitro, both RRVs expressing the green fluorescent protein gene efficiently replicated in most mesothelioma cell lines tested, but not in normal mesothelial cells. Notably, in ACC-MESO-1 mesothelioma cells that were not permissive for AMLV-RRV, the GALV-RRV could spread efficiently in culture and in mice with subcutaneous xenografts by in vivo fluorescence imaging. Next, GALV-RRV expressing the cytosine deaminase prodrug activator gene showed efficient killing of ACC-MESO-1 cells in a prodrug 5-fluorocytosine dose-dependent manner, compared with AMLV-RRV. GALV-RRV-mediated prodrug activator gene therapy achieved significant inhibition of subcutaneous ACC-MESO-1 tumor growth in nude mice. Quantitative reverse transcription PCR demonstrated that ACC-MESO-1 cells express higher PiT-1 (GALV receptor) and lower PiT-2 (AMLV receptor) compared with normal mesothelial cells and other mesothelioma cells, presumably accounting for the distinctive finding that GALV-RRV replicates much more robustly than AMLV-RRV in these cells. These data indicate the potential utility of GALV-RRV-mediated prodrug activator gene therapy in the treatment of mesothelioma.

  11. Coating urchinlike gold nanoparticles with polypyrrole thin shells to produce photothermal agents with high stability and photothermal transduction efficiency.

    PubMed

    Li, Jing; Han, Jishu; Xu, Tianshu; Guo, Changrun; Bu, Xinyuan; Zhang, Hao; Wang, Liping; Sun, Hongchen; Yang, Bai

    2013-06-11

    Photothermal therapy using inorganic nanoparticles (NPs) is a promising technique for the selective treatment of tumor cells because of their capability to convert the absorbed radiation into heat energy. Although anisotropic gold (Au) NPs present an excellent photothermal effect, the poor structural stability during storage and/or upon laser irradiation still limits their practical application as efficient photothermal agents. With the aim of improving the stability, in this work we adopted biocompatible polypyrrole (PPy) as the shell material for coating urchinlike Au NPs. The experimental results indicate that a several nanometer PPy shell is enough to maintain the structural stability of NPs. In comparison to the bare NPs, PPy-coated NPs exhibit improved structural stability toward storage, heat, pH, and laser irradiation. In addition, the thin shell of PPy also enhances the photothermal transduction efficiency (η) of PPy-coated Au NPs, resulting from the absorption of PPy in the red and near-infrared (NIR) regions. For example, the PPy-coated Au NPs with an Au core diameter of 120 nm and a PPy shell of 6.0 nm exhibit an η of 24.0% at 808 nm, which is much higher than that of bare Au NPs (η = 11.0%). As a primary attempt at photothermal therapy, the PPy-coated Au NPs with a 6.0 nm PPy shell exhibit an 80% death rate of Hela cells under 808 nm NIR laser irradiation.

  12. Intradermal delivery of adenoviral type-35 vectors leads to high efficiency transduction of mature, CD8+ T cell-stimulating skin-emigrated dendritic cells.

    PubMed

    de Gruijl, Tanja D; Ophorst, Olga J A E; Goudsmit, Jaap; Verhaagh, Sandra; Lougheed, Sinéad M; Radosevic, Katarina; Havenga, Menzo J E; Scheper, Rik J

    2006-08-15

    Recombinant adenovirus (Ad) type 35 (rAd35) shows great promise as vaccine carrier with the advantage of low pre-existing immunity in human populations, in contrast to the more commonly used rAd5 vector. The rAd35 vector uses CD46 as a high-affinity receptor, which, unlike the rAd5 receptor, is expressed on human dendritic cells (DC), the most powerful APCs identified to date. In this study, we show that in contrast to rAd5, rAd35 infects migrated and mature CD83+ cutaneous DC with high efficiency (up to 80%), when delivered intradermally in an established human skin explant model. The high transduction efficiency is in line with high expression levels of CD46 detected on migratory cutaneous DC, which proved to be further increased upon intradermal administration of GM-CSF and IL-4. As compared with Ad5, these Ad35 infection characteristics translate into higher absolute numbers of skin-emigrated DC per explant that both express the transgene and are phenotypically mature. Finally, we demonstrate that upon intracutaneous delivery of a rAd35 vaccine encoding the circumsporozoite (CS) protein of Plasmodium falciparum, emigrated DC functionally express and process CS-derived epitopes and are capable of activating specific CD8+ effector T cells, as evidenced by activation of an HLA-A2-restricted CS-specific CD8+ T cell clone. Collectively, these data demonstrate the utility of rAd35 vectors for efficient in vivo human DC transduction.

  13. Pip5 Transduction Peptides Direct High Efficiency Oligonucleotide-mediated Dystrophin Exon Skipping in Heart and Phenotypic Correction in mdx Mice

    PubMed Central

    Yin, HaiFang; Saleh, Amer F; Betts, Corinne; Camelliti, Patrizia; Seow, Yiqi; Ashraf, Shirin; Arzumanov, Andrey; Hammond, Suzan; Merritt, Thomas; Gait, Michael J; Wood, Matthew JA

    2011-01-01

    Induced splice modulation of pre-mRNAs shows promise to correct aberrant disease transcripts and restore functional protein and thus has therapeutic potential. Duchenne muscular dystrophy (DMD) results from mutations that disrupt the DMD gene open reading frame causing an absence of dystrophin protein. Antisense oligonucleotide (AO)-mediated exon skipping has been shown to restore functional dystrophin in mdx mice and DMD patients treated intramuscularly in two recent phase 1 clinical trials. Critical to the therapeutic success of AO-based treatment will be the ability to deliver AOs systemically to all affected tissues including the heart. Here, we report identification of a series of transduction peptides (Pip5) as AO conjugates for enhanced systemic and particularly cardiac delivery. One of the lead peptide-AO conjugates, Pip5e-AO, showed highly efficient exon skipping and dystrophin production in mdx mice with complete correction of the aberrant DMD transcript in heart, leading to >50% of the normal level of dystrophin in heart. Mechanistic studies indicated that the enhanced activity of Pip5e-phosphorodiamidate morpholino (PMO) is partly explained by more efficient nuclear delivery. Pip5 series derivatives therefore have significant potential for advancing the development of exon skipping therapies for DMD and may have application for enhanced cardiac delivery of other biotherapeutics. PMID:21505427

  14. Optimization of the transductional efficiency of lentiviral vectors: effect of sera and polycations.

    PubMed

    Denning, Warren; Das, Suvendu; Guo, Siqi; Xu, Jun; Kappes, John C; Hel, Zdenek

    2013-03-01

    Lentiviral vectors are widely used as effective gene-delivery vehicles. Optimization of the conditions for efficient lentiviral transduction is of a high importance for a variety of research applications. Presence of positively charged polycations reduces the electrostatic repulsion forces between a negatively charged cell and an approaching enveloped lentiviral particle resulting in an increase in the transduction efficiency. Although a variety of polycations are commonly used to enhance the transduction with retroviruses, the relative effect of various types of polycations on the efficiency of transduction and on the potential bias in the determination of titer of lentiviral vectors is not fully understood. Here, we present data suggesting that DEAE-dextran provides superior results in enhancing lentiviral transduction of most tested cell lines and primary cell cultures. Specific type and source of serum affects the efficiency of transduction of target cell populations. Non-specific binding of enhanced green fluorescent protein (EGFP)-containing membrane aggregates in the presence of DEAE-dextran does not significantly affect the determination of the titer of EGFP-expressing lentiviral vectors. In conclusion, various polycations and types of sera should be tested when optimizing lentiviral transduction of target cell populations.

  15. High-sensitivity linear piezoresistive transduction for nanomechanical beam resonators

    NASA Astrophysics Data System (ADS)

    Sansa, Marc; Fernández-Regúlez, Marta; Llobet, Jordi; San Paulo, Álvaro; Pérez-Murano, Francesc

    2014-07-01

    Highly sensitive conversion of motion into readable electrical signals is a crucial and challenging issue for nanomechanical resonators. Efficient transduction is particularly difficult to realize in devices of low dimensionality, such as beam resonators based on carbon nanotubes or silicon nanowires, where mechanical vibrations combine very high frequencies with miniscule amplitudes. Here we describe an enhanced piezoresistive transduction mechanism based on the asymmetry of the beam shape at rest. We show that this mechanism enables highly sensitive linear detection of the vibration of low-resistivity silicon beams without the need of exceptionally large piezoresistive coefficients. The general application of this effect is demonstrated by detecting multiple-order modes of silicon nanowire resonators made by either top-down or bottom-up fabrication methods. These results reveal a promising approach for practical applications of the simplest mechanical resonators, facilitating its manufacturability by very large-scale integration technologies.

  16. Increased transduction efficiency of primary hematopoietic cells by physical colocalization of retrovirus and target cells.

    PubMed

    Hutchings, M; Moriwaki, K; Dilloo, D; Hoffmann, T; Kimbrough, S; Johnsen, H E; Brenner, M K; Heslop, H E

    1998-06-01

    Efficient gene transfer into hematopoietic stem cells offers a number of potential therapeutic applications. However, the relatively low titer of retroviral supernatants and the requirement for cell division to ensure integration have meant that transduction efficiency has been low. We have modified a flowthrough approach to cell transduction and have been able consistently to increase gene transfer efficiency into human hematopoietic progenitor cells. We transduced CD34 cells with retroviral vectors encoding a truncated nerve growth factor receptor (NGFR) or neo. Retroviral supernatant was pulled through 0.2-micron polycarbonated membranes, followed by placement of cells on the filter. In the absence of cytokines, the transduction efficiency of CD34 cells with a NGFR vector was increased 3-11-fold over that obtained at an identical MOI in liquid culture to produce 11%-44% transduction. Furthermore, both Thy1+ and Thy1- subsets in a total CD34 population were transduced with similar efficiency, and transduction with a neo vector, as measured by G418 resistance in clonogenic assays, increased 1.5-5-fold. The mechanism by which gene transfer is improved may reflect colocalization of cells and retrovirus. Costaining of cells transduced on the filter with an NGFR retrovirus with both an NGFR antibody and a gp70 antibody that recognizes viral coat protein revealed high-level coexpression. The levels of in vitro gene transfer we obtain are equivalent to those observed when CD34 cells are cocultured in liquid culture with cytokines. However, culture with cytokines may commit CD34 cells to differentiation and has produced disappointingly low levels of subsequent in vivo gene transfer. Gene marking studies using distinguishable retroviral vectors will provide a means of learning whether the effects of flowthrough transduction genuinely enhance the efficiency of gene transfer to human marrow-repopulating cells.

  17. Efficient lentiviral gene transfer to canine repopulating cells using an overnight transduction protocol.

    PubMed

    Horn, Peter A; Keyser, Kirsten A; Peterson, Laura J; Neff, Tobias; Thomasson, Bobbie M; Thompson, Jesse; Kiem, Hans-Peter

    2004-05-15

    The use of lentiviral vectors for the transduction of hematopoietic stem cells has evoked much interest owing to their ability to stably integrate into the genome of nondividing cells. However, published large animal studies have reported highly variable gene transfer rates of typically less than 1%. Here we report the use of lentiviral vectors for the transduction of canine CD34(+) hematopoietic repopulating cells using a very short, 18-hour transduction protocol. We compared lentiviral transduction of hematopoietic repopulating cells from either stem cell factor (SCF)- and granulocyte-colony stimulating factor (G-CSF)-primed marrow or mobilized peripheral blood in a competitive repopulation assay in 3 dogs. All dogs engrafted rapidly within 9 days. Transgene expression was detected in all lineages (B cells, T cells, granulocytes, and red blood cells as well as platelets) indicating multilineage engraftment of transduced cells, with overall long-term marking levels of up to 12%. Gene transfer levels in mobilized peripheral blood cells were slightly higher than in primed marrow cells. In conclusion, we show efficient lentiviral transduction of canine repopulating cells using an overnight transduction protocol. These results have important implications for the design of stem cell gene therapy protocols, especially for those diseases in which the maintenance of stem cells in culture is a major limitation.

  18. A novel semi-transductive learning framework for efficient atypicality detection in chest radiographs

    NASA Astrophysics Data System (ADS)

    Alzubaidi, Mohammad; Balasubramanian, Vineeth; Patel, Ameet; Panchanathan, Sethuraman; Black, John A., Jr.

    2012-03-01

    Inductive learning refers to machine learning algorithms that learn a model from a set of training data instances. Any test instance is then classified by comparing it to the learned model. When the set of training instances lend themselves well to modeling, the use of a model substantially reduces the computation cost of classification. However, some training data sets are complex, and do not lend themselves well to modeling. Transductive learning refers to machine learning algorithms that classify test instances by comparing them to all of the training instances, without creating an explicit model. This can produce better classification performance, but at a much higher computational cost. Medical images vary greatly across human populations, constituting a data set that does not lend itself well to modeling. Our previous work showed that the wide variations seen across training sets of "normal" chest radiographs make it difficult to successfully classify test radiographs with an inductive (modeling) approach, and that a transductive approach leads to much better performance in detecting atypical regions. The problem with the transductive approach is its high computational cost. This paper develops and demonstrates a novel semi-transductive framework that can address the unique challenges of atypicality detection in chest radiographs. The proposed framework combines the superior performance of transductive methods with the reduced computational cost of inductive methods. Our results show that the proposed semitransductive approach provides both effective and efficient detection of atypical regions within a set of chest radiographs previously labeled by Mayo Clinic expert thoracic radiologists.

  19. Confocal Scanner for Highly Sensitive Photonic Transduction of Nanomechanical Resonators

    NASA Astrophysics Data System (ADS)

    Diao, Zhu; Losby, Joseph E.; Sauer, Vincent T. K.; Westwood, Jocelyn N.; Freeman, Mark R.; Hiebert, Wayne K.

    2013-06-01

    We show that a simple confocal laser scanning system can be used to couple light through grating couplers into nanophotonic circuits. The coupling efficiency is better than 15% per coupler. Our technique avoids using multi-axis fibre stages and is especially advantageous when the nanophotonic circuit is kept in vacuum, e.g., for nanomechanical resonator displacement transduction. This was demonstrated by recording the resonant response of a nanomechanical doubly clamped beam embedded in a race-track optical cavity. The nanophotonic transduction offers an increase of two orders of magnitude in transduction responsivity compared with conventional free-space optical interferometry.

  20. Effects of pergolide mesylate on transduction efficiency of PEP-1-catalase protein

    SciTech Connect

    Sohn, Eun Jeong; Kim, Dae Won; Kim, Young Nam; Kim, So Mi; Lim, Soon Sung; Kang, Tae-Cheon; Kwon, Hyeok Yil; Kim, Duk-Soo; Cho, Sung-Woo; Han, Kyu Hyung; Park, Jinseu; Eum, Won Sik; Hwang, Hyun Sook; Choi, Soo Young

    2011-03-18

    Research highlights: {yields} We studied effects of pergolide mesylate (PM) on in vitro and in vivo transduction of PEP-1-catalase. {yields} PEP-1-catatase inhibited 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced inflammation. {yields} PM enhanced the transduction of PEP-1-catalase into HaCaT cells and skin tissue. {yields} PM increased anti-inflammatory activity of PEP-1-catalase. {yields} PM stimulated therapeutic action of anti-oxidant enzyme catalase in oxidative-related diseases. -- Abstract: The low transduction efficiency of various proteins is an obstacle to their therapeutic application. However, protein transduction domains (PTDs) are well-known for a highly effective tool for exogenous protein delivery to cells. We examined the effects of pergolide mesylate (PM) on the transduction of PEP-1-catalase into HaCaT human keratinocytes and mice skin and on the anti-inflammatory activity of PEP-1-catatase against 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced inflammation using Western blot and histological analysis. PM enhanced the time- and dose-dependent transduction of PEP-1-catalase into HaCaT cells without affecting the cellular toxicity. In a mouse edema model, PEP-1-catalase inhibited the increased expressions of inflammatory mediators and cytokines such as cyclooxygenase-2, inducible nitric oxide synthase, interleukin-6 and -1{beta}, and tumor necrosis factor-{alpha} induced by TPA. On the other hand, PM alone failed to exert any significant anti-inflammatory effects. However, the anti-inflammatory effect of co-treatment with PEP-1-catalase and PM was more potent than that of PEP-1-catalase alone. Our results indicate that PM may enhance the delivery of PTDs fusion therapeutic proteins to target cells and tissues and has potential to increase their therapeutic effects of such drugs against various diseases.

  1. High efficiency retroviral mediated gene transduction into single isolated immature and replatable CD34(3+) hematopoietic stem/progenitor cells from human umbilical cord blood

    PubMed Central

    1993-01-01

    Umbilical cord blood is rich in hematopoietic stem and progenitor cells and has recently been used successfully in the clinic as an alternative source of engrafting and marrow repopulating cells. With the likelihood that cord blood stem/progenitor cells will be used for gene therapy to correct genetic disorders, we evaluated if a TK-neo gene could be directly transduced in a stable manner into single isolated subsets of purified immature hematopoietic cells that demonstrate self-renewed ability as estimated by colony replating capacity. Sorted CD34(3+) cells from cord blood were prestimulated with erythropoietin (Epo), steel factor (SLF), interleukin (IL)-3, and granulocyte-macrophage colony stimulating factor (GM-CSF) and transduced with the gene in two ways. CD34(3+) cells were incubated with retroviral-containing supernatant from TK-neo vector-producing cells, washed, and plated directly or resorted as CD34(3+) cells into single wells containing a single cell or 10 cells. Alternatively, CD34(3+) cells were sorted as a single cell/well and then incubated with viral supernatant. These cells were cultured with Epo, SLF, IL-3, and GM-CSF +/- G418. The TK-neo gene was introduced at very high efficiency into low numbers of or isolated single purified CD34(3+) immature hematopoietic cells without stromal cells as a source of virus or accessory cells. Proviral integration was detected in primary G418-resistant(R) colonies derived from single immature hematopoietic cells, and in cells from replated colonies derived from G418R-colony forming unit-granulocyte erythroid macrophage megakaryocyte (CFU-GEMM) and -high proliferative potential colony forming cells (HPP-CFC). This demonstrates stable expression of the transduced gene into single purified stem/progenitor cells with replating capacity, results that should be applicable for future clinical studies that may utilize selected subsets of stem/progenitor cells for gene therapy. PMID:7504056

  2. Membrane penetrating peptides greatly enhance baculovirus transduction efficiency into mammalian cells

    SciTech Connect

    Chen, Hong-Zhang; Wu, Carol P.; Chao, Yu-Chan; Liu, Catherine Yen-Yen

    2011-02-11

    Research highlights: {yields} Ligation of CTP with GP64 enhances baculovirus transduction into mammalian cells. {yields} Fusion of PTD with VP39 enhances baculovirus transduction into mammalian cells. {yields} CTP and PTD-carrying viruses improve the transduction of co-transduced baculoviruses. {yields} Virus entry and gene expression can be separate events in different cell types. -- Abstract: The baculovirus group of insect viruses is widely used for foreign gene introduction into mammalian cells for gene expression and protein production; however, the efficiency of baculovirus entry into mammalian cells is in general still low. In this study, two recombinant baculoviruses were engineered and their ability to improve viral entry was examined: (1) cytoplasmic transduction peptide (CTP) was fused with baculovirus envelope protein, GP64, to produce a cytoplasmic membrane penetrating baculovirus (vE-CTP); and (2) the protein transduction domain (PTD) of HIV TAT protein was fused with the baculovirus capsid protein VP39 to form a nuclear membrane penetrating baculovirus (vE-PTD). Transduction experiments showed that both viruses had better transduction efficiency than vE, a control virus that only expresses EGFP in mammalian cells. Interestingly, vE-CTP and vE-PTD were also able to improve the transduction efficiency of a co-transduced baculovirus, resulting in higher levels of gene expression. Our results have described new routes to further enhance the development of baculovirus as a tool for gene delivery into mammalian cells.

  3. Pseudotyped adeno-associated viral vector tropism and transduction efficiencies in murine wound healing.

    PubMed

    Keswani, Sundeep G; Balaji, Swathi; Le, Louis; Leung, Alice; Lim, Foong-Yen; Habli, Mounira; Jones, Helen N; Wilson, James M; Crombleholme, Timothy M

    2012-01-01

    Cell specific gene transfer and sustained transgene expression are goals of cutaneous gene therapy for tissue repair and regeneration. Adeno-associated virus serotype 2 (AAV2/2) mediated gene transfer to the skin results in stable transgene expression in the muscle fascicles of the panniculus carnosus in mice, with minimal gene transfer to the dermal or epidermal elements. We hypothesized that pseudotyped AAV vectors may have a unique and characteristic tropism and transduction efficiency profile for specific cells in the cutaneous wounds. We compared transduction efficiencies of cells in the epidermis, cells in the dermis, and the fascicles of the panniculus carnosus by AAV2/2 and three pseudotyped AAV vectors, AAV2/5, AAV2/7, and AAV2/8 in a murine excisional wound model. AAV2/5 and AAV2/8 result in significantly enhanced transduction of cells both in the epidermis and the dermis compared to AAV2/2. AAV2/5 transduces both the basilar and supra-basilar keratinocytes. In contrast, AAV2/8 transduces mainly supra-basilar keratinocytes. Both AAV2/7 and AAV2/8 result in more efficient gene transfer to the muscular panniculus carnosus compared to AAV2/2. The capsid of the different pseudotyped AAV vectors produces distinct tropism and efficiency profiles in the murine wound healing model. Both AAV2/5 and AAV2/8 administration result in significantly enhanced gene transfer. To further characterize cell specific transduction and tropism profiles of the AAV pseudotyped vectors, we performed in vitro experiments using human and mouse primary dermal fibroblasts. Our data demonstrate that pseudotyping strategy confers a differential transduction of dermal fibroblasts, with higher transduction of both human and murine cells by AAV2/5 and AAV2/8 at early and later time points. At later time points, AAV2/2 demonstrates increased transduction. Interestingly, AAV2/8 appears to be more efficacious in transducing human cells as compared to AAV2/5. The pseudotype-specific pattern of

  4. An Efficient Large-Scale Retroviral Transduction Method Involving Preloading the Vector into a RetroNectin-Coated Bag with Low-Temperature Shaking

    PubMed Central

    Dodo, Katsuyuki; Chono, Hideto; Saito, Naoki; Tanaka, Yoshinori; Tahara, Kenichi; Nukaya, Ikuei; Mineno, Junichi

    2014-01-01

    In retroviral vector-mediated gene transfer, transduction efficiency can be hampered by inhibitory molecules derived from the culture fluid of virus producer cell lines. To remove these inhibitory molecules to enable better gene transduction, we had previously developed a transduction method using a fibronectin fragment-coated vessel (i.e., the RetroNectin-bound virus transduction method). In the present study, we developed a method that combined RetroNectin-bound virus transduction with low-temperature shaking and applied this method in manufacturing autologous retroviral-engineered T cells for adoptive transfer gene therapy in a large-scale closed system. Retroviral vector was preloaded into a RetroNectin-coated bag and incubated at 4°C for 16 h on a reciprocating shaker at 50 rounds per minute. After the supernatant was removed, activated T cells were added to the bag. The bag transduction method has the advantage of increasing transduction efficiency, as simply flipping over the bag during gene transduction facilitates more efficient utilization of the retroviral vector adsorbed on the top and bottom surfaces of the bag. Finally, we performed validation runs of endoribonuclease MazF-modified CD4+ T cell manufacturing for HIV-1 gene therapy and T cell receptor-modified T cell manufacturing for MAGE-A4 antigen-expressing cancer gene therapy and achieved over 200-fold (≥1010) and 100-fold (≥5×109) expansion, respectively. In conclusion, we demonstrated that the large-scale closed transduction system is highly efficient for retroviral vector-based T cell manufacturing for adoptive transfer gene therapy, and this technology is expected to be amenable to automation and improve current clinical gene therapy protocols. PMID:24454964

  5. TRIM5α variations influence transduction efficiency with lentiviral vectors in both human and rhesus CD34(+) cells in vitro and in vivo.

    PubMed

    Evans, Molly E; Kumkhaek, Chutima; Hsieh, Matthew M; Donahue, Robert E; Tisdale, John F; Uchida, Naoya

    2014-02-01

    Human immunodeficiency virus type 1 (HIV-1) vectors can transduce human hematopoietic stem cells (HSC), but transduction efficiency varies among individuals. The innate immune factor tripartite motif-containing protein 5α (TRIM5α) plays an important role for restriction of retroviral infection. In this study, we examined whether TRIM5α could account for variations in transduction efficiency using both an established rhesus gene therapy model and human CD34(+) cell culture. Evaluation of TRIM5α genotypes (Mamu-1, -2, -3, -4, -5, and TrimCyp) in 16 rhesus macaques that were transplanted with transduced CD34(+) cells showed a significant correlation between TRIM5α Mamu-4 and high gene marking in both lymphocytes and granulocytes 6 months after transplantation. Since significant human TRIM5α coding polymorphisms were not known, we evaluated TRIM5α expression levels in human CD34(+) cells from 14 donors. Three days after HIV-1 vector transduction, measured transduction efficiency varied significantly among donors and was negatively correlated with TRIM5α expression levels. In summary, transduction efficiency in both rhesus and human CD34(+) cells was influenced by TRIM5α variations (genotypes and expression levels). Our findings are important for both understanding and mitigating the variability of transduction efficiency for rhesus and human CD34(+) cells.

  6. Efficient preparation of cationized gelatin for gene transduction.

    PubMed

    Fukuyama, Naoto; Onuma, Tsuyoshi; Jujo, Shio; Tamai, Yoshifumi; Suzuki, Takahiro; Myojin, Kazunori; Tabata, Yasuhiko; Ishihara, Yoshimi; Takano, Jiro; Mori, Hidezo

    2006-07-20

    We previously reported gene therapy using cationized gelatin microspheres of φ20-32 μm, prepared from pig skin, as a transducing agent, but although the gelatin offered various advantages, its yield was extremely low (only 0.1%). In this study, we markedly improved the yield of φ20-32 μm cationized gelatin microspheres and prepared a newly less than φ20 μm cationized gelatin. Conventionally, cationized gelatin is prepared by cationization, particulation by agitation, and cross-linking. The yield is determined by the particulation step, for which we had used a three-necked distillation flask of 500 mL and an agitation speed of 420 rpm. The yield was significantly increased from 0.13 ± 0.02% to 8.80 ± 1.90% by using a smaller flask of 300 mL and an agitation speed of 25000 rpm (p < 0.01). We could also prepare cationized gelatin of less than φ20 μm, which had not been possible previously. We confirmed that efficient gene introduction into peritoneal macrophages could be achieved with the new cationized gelatin.

  7. Multilayered films fabricated from an oligoarginine-conjugated protein promote efficient surface-mediated protein transduction.

    PubMed

    Jewell, Christopher M; Fuchs, Stephen M; Flessner, Ryan M; Raines, Ronald T; Lynn, David M

    2007-03-01

    The conjugation of cationic protein transduction domains to proteins results in an increase in the extent to which proteins are internalized by cells. This investigation sought to determine whether the conjugation of a protein transduction domain to a functional protein could be used to facilitate the incorporation of the protein into multilayered polyelectrolyte films and, subsequently, whether these films could be used to promote surface-mediated protein transduction. We demonstrate that it is possible to fabricate multilayered assemblies 80 nm thick using sodium polystyrene sulfonate (SPS) and bovine pancreatic ribonuclease (RNase A) conjugated to the cationic protein transduction domain nonaarginine (R(9)) using an entirely aqueous layer-by-layer process. We demonstrate further that the conjugation of R(9) to RNase A permits the assembly of multilayered films under conditions that do not allow for the incorporation of the unmodified protein. This result suggests that R(9) functions as a cationic anchor and serves to increase the strength of electrostatic interactions with SPS and facilitate layer-by-layer assembly. We also demonstrate that RNase A-R(9)/SPS films dissolve rapidly in physiologically relevant media and that macroscopic objects coated with these materials can be used to mediate high levels of protein transduction in mammalian cells. These results suggest the basis of general methods that could contribute to the design of materials that permit spatial and temporal control over the delivery of therapeutic proteins to cells and tissues.

  8. Branched oligomerization of cell-permeable peptides markedly enhances the transduction efficiency of adenovirus into mesenchymal stem cells.

    PubMed

    Park, S-H; Doh, J; Park, S I; Lim, J Y; Kim, S M; Youn, J-I; Jin, H-T; Seo, S-H; Song, M-Y; Sung, S Y; Kim, M; Hwang, S J; Choi, J-M; Lee, S-K; Lee, H Y; Lim, C L; Chung, Y J; Yang, D; Kim, H-N; Lee, Z H; Choi, K Y; Jeun, S-S; Sung, Y C

    2010-08-01

    Cell-permeable peptides (CPPs) promote the transduction of nonpermissive cells by recombinant adenovirus (rAd) to improve the therapeutic efficacy of rAd. In this study, branched oligomerization of CPPs significantly enhanced the transduction of human mesenchymal stem cells (MSCs) by rAd in a CPP type-independent manner. In particular, tetrameric CPPs increased transduction efficiency at 3000-5000-fold lower concentrations than did monomeric CPPs. Although branched oligomerization of CPPs also increases cytotoxicity, optimal concentrations of tetrameric CPPs required for maximum transduction are at least 300-1000-fold lower than those causing 50% cytotoxicity. Furthermore, although only approximately 60% of MSCs were maximally transduced at 500 muM of monomeric CPPs, >95% of MSCs were transduced with 0.1 muM of tetrameric CPPs. Tetrameric CPPs also significantly increased the formation and net surface charge of CPP/rAd complexes, as well as the binding of rAd to cell membranes at a greater degree than did monomeric CPPs, followed by rapid internalization into MSCs. In a critical-size calvarial defect model, the inclusion of tetrameric CPPs in ex vivo transduction of rAd expressing bone morphogenetic protein 2 into MSCs promoted highly mineralized bone formation. In addition, MSCs that were transduced with rAd expressing brain-derived neurotrophic factor in the presence of tetrameric CPPs improved functional recovery in a spinal cord injury model. These results demonstrated the potential for tetrameric CPPs to provide an innovative tool for MSC-based gene therapy and for in vitro gene delivery to MSCs.

  9. Serotype-dependent transduction efficiencies of recombinant adeno-associated viral vectors in monkey neocortex

    PubMed Central

    Gerits, Annelies; Vancraeyenest, Pascaline; Vreysen, Samme; Laramée, Marie-Eve; Michiels, Annelies; Gijsbers, Rik; Van den Haute, Chris; Moons, Lieve; Debyser, Zeger; Baekelandt, Veerle; Arckens, Lutgarde; Vanduffel, Wim

    2015-01-01

    Abstract. Viral vector-mediated expression of genes (e.g., coding for opsins and designer receptors) has grown increasingly popular. Cell-type specific expression is achieved by altering viral vector tropism through crosspackaging or by cell-specific promoters driving gene expression. Detailed information about transduction properties of most recombinant adeno-associated viral vector (rAAV) serotypes in macaque cortex is gradually becoming available. Here, we compare transduction efficiencies and expression patterns of reporter genes in two macaque neocortical areas employing different rAAV serotypes and promoters. A short version of the calmodulin-kinase-II (CaMKIIα0.4) promoter resulted in reporter gene expression in cortical neurons for all tested rAAVs, albeit with different efficiencies for spread: rAAV2/5>>rAAV2/7>rAAV2/8>rAAV2/9>>rAAV2/1 and proportion of transduced cells: rAAV2/1>rAAV2/5>rAAV2/7=rAAV2/9>rAAV2/8. In contrast to rodent studies, the cytomegalovirus (CMV) promoter appeared least efficient in macaque cortex. The human synapsin-1 promoter preceded by the CMV enhancer (enhSyn1) produced homogeneous reporter gene expression across all layers, while two variants of the CaMKIIα promoter resulted in different laminar transduction patterns and cell specificities. Finally, differences in expression patterns were observed when the same viral vector was injected in two neocortical areas. Our results corroborate previous findings that reporter-gene expression patterns and efficiency of rAAV transduction depend on serotype, promoter, cortical layer, and area. PMID:26839901

  10. Physiological performance of warm-adapted marine ectotherms: Thermal limits of mitochondrial energy transduction efficiency.

    PubMed

    Martinez, Eloy; Hendricks, Eric; Menze, Michael A; Torres, Joseph J

    2016-01-01

    Thermal regimes in aquatic systems have profound implications for the physiology of ectotherms. In particular, the effect of elevated temperatures on mitochondrial energy transduction in tropical and subtropical teleosts may have profound consequences on organismal performance and population viability. Upper and lower whole-organism critical temperatures for teleosts suggest that subtropical and tropical species are not susceptible to the warming trends associated with climate change, but sub-lethal effects on energy transduction efficiency and population dynamics remain unclear. The goal of the present study was to compare the thermal sensitivity of processes associated with mitochondrial energy transduction in liver mitochondria from the striped mojarra (Eugerres plumieri), the whitemouth croaker (Micropogonias furnieri) and the palometa (Trachinotus goodei), to those of the subtropical pinfish (Lagodon rhomboides) and the blue runner (Caranx crysos). Mitochondrial function was assayed at temperatures ranging from 10 to 40°C and results obtained for both tropical and subtropical species showed a reduction in the energy transduction efficiency of the oxidative phosphorylation (OXPHOS) system in most species studied at temperatures below whole-organism critical temperature thresholds. Our results show a loss of coupling between O2 consumption and ATP production before the onset of the critical thermal maxima, indicating that elevated temperature may severely impact the yield of ATP production per carbon unit oxidized. As warming trends are projected for tropical regions, increasing water temperatures in tropical estuaries and coral reefs could impact long-term growth and reproductive performance in tropical organisms, which are already close to their upper thermal limit. Copyright © 2015 Elsevier Inc. All rights reserved.

  11. Concurrent measures of fusion and transduction efficiency of primary CD34+ cells with human immunodeficiency virus 1-based lentiviral vectors reveal different effects of transduction enhancers.

    PubMed

    Ingrao, Dina; Majdoul, Saliha; Seye, Ababacar K; Galy, Anne; Fenard, David

    2014-02-01

    Lentiviral vectors (LVs) are used for various gene transfer applications, notably for hematopoietic gene therapy, but methods are lacking for precisely evaluating parameters that control the efficiency of transduction in relation to the entry of vectors into target cells. We adapted a fluorescence resonance energy transfer-based human immunodeficiency virus-1 fusion assay to measure the entry of nonreplicative recombinant LVs in various cell types, including primary human hematopoietic stem progenitor cells (HSPCs), and to quantify the level of transduction of the same initially infected cells. The assay utilizes recombinant LVs containing β-lactamase (BLAM)-Vpr chimeric proteins (BLAM-LVs) and encoding a truncated form of the low-affinity nerve growth factor receptor (ΔNGFR). After infection of target cells with BLAM-LVs, the vector entry rapidly leads to BLAM-Vpr release into the cytoplasm, which is measured by cleavage of a fluorescent substrate using flow cytometry. Parallel cultures of the same infected cells show transduction efficiency resulting from ΔNGFR expression. This LV-based fusion/transduction assay is a dynamic and versatile tool, revealing, for instance, the postentry restrictions of LVs known to occur in cells of hematopoietic origin, especially human HSPCs. Furthermore, this BLAM-LV assay allowed us to evaluate the effect of cytokine prestimulation of HSPCs on the entry step of LVs. The assay also shows that transduction enhancers such as Vectofusin-1 or Retronectin can partially relieve the postentry block, but their effects differ in how they promote LV entry. In conclusion, one such assay should be useful to study hematopoietic postentry restrictions directed against LVs and therefore should allow improvements in various LV-based gene therapy protocols.

  12. Efficient Gene Transduction of Dispersed Islet Cells in Culture Using Fiber-Modified Adenoviral Vectors

    PubMed Central

    Hanayama, Hiroyuki; Ohashi, Kazuo; Utoh, Rie; Shimizu, Hirofumi; Ise, Kazuya; Sakurai, Fuminori; Mizuguchi, Hiroyuki; Tsuchiya, Hiroyuki; Okano, Teruo; Gotoh, Mitsukazu

    2015-01-01

    To establish novel islet-based therapies, our group has recently developed technologies for creating functional neo-islet tissues in the subcutaneous space by transplanting monolithic sheets of dispersed islet cells (islet cell sheets). Improving cellular function and viability are the next important challenges for enhancing the therapeutic effects. This article describes the adenoviral vector-mediated gene transduction of dispersed islet cells under culture conditions. Purified pancreatic islets were obtained from Lewis rats and dissociated into single islet cells. Cells were plated onto laminin-5-coated temperature-responsive polymer poly(N-isopropylacrylamide)-immobilized plastic dishes. At 0 h, islet cells were infected for 1 h with either conventional type 5 adenoviral vector (Ad-CA-GFP) or fiber-modified adenoviral vector (AdK7-CA-GFP) harboring a polylysine (K7) peptide in the C terminus of the fiber knob. We investigated gene transduction efficiency at 48 h after infection and found that AdK7-CA-GFP yielded higher transduction efficiencies than Ad-CA-GFP at a multiplicity of infection (MOI) of 5 and 10. For AdK7-CA-GFP at MOI = 10, 84.4 ± 1.5% of islet cells were found to be genetically transduced without marked vector infection-related cellular damage as determined by viable cell number and lactate dehydrogenase (LDH) release assay. After AdK7-CA-GFP infection at MOI = 10, cells remained attached and expanded to nearly full confluency, showing that this adenoviral infection protocol is a feasible approach for creating islet cell sheets. We have shown that dispersed and cultured islet cells can be genetically modified efficiently using fiber-modified adenoviral vectors. Therefore, this gene therapy technique could be used for cellular modification or biological assessment of dispersed islet cells. PMID:26858906

  13. [Expression, purification, stability and transduction efficiency of GST-SOD1-R9 fusion protein].

    PubMed

    Pan, Jianru; Wu, Lunqiao; He, Huocong; Chen, Lijuan; Su, Ying; Li, Lingling; Liu, Shutao

    2017-05-25

    The fusion of cell permeable peptide TAT and bifunctional antioxidant enzymes, GST (Glutathione sulfur transferase)-TAT-SOD1 (Cu, Zn superoxide dismutase), is an intracellular superoxide scavenger. Compared with SOD1-TAT, GST-TAT-SOD1 has better protective effect on oxidative damage but less transduction efficiency. A novel cell permeable bifunctional antioxidant enzymes with the fusion of GST, SOD1 and polyarginine R9 was constructed for higher transduction efficiency. The full nucleotide sequence of SOD1-R9 was synthesized and inserted into the prokaryotic expression vector pGEX-4T-1 with the GST tag. After the successful construction of the prokaryotic expression vectors of GST-SOD1-R9, the recombinant vector was then transformed into Escherichia coli BL21 (DE3) and the GST-SOD1-R9 fusion protein was produced with the induction of IPTG. The soluble expression of GST-SOD1-R9 fusion protein was combining with the induction temperature and time. The best soluble expression was obtained with the induction temperature of 25 ℃ and the induction time of 11 h. The fusion protein was purified through the combination of 80% ammonium sulfate precipitation and affinity chromatography using glutathione agarose, and verified by SDS-PAGE and special enzymatic activity. The thermal and pH stability of GST-SOD1-R9 fusion protein were analyzed and the SOD and GST activity of fusion protein were proved to be well maintained under physiological conditions. Finally, the transduction efficiency of GST-SOD1-R9 fusion protein was proved to be better than GST-TAT-SOD1 fusion protein (P<0.05). These works establish a foundation for further study of the protective effect of GST-SOD1-R9 fusion protein against oxidative damage.

  14. Efficient and Targeted Transduction of Nonhuman Primate Liver With Systemically Delivered Optimized AAV3B Vectors.

    PubMed

    Li, Shaoyong; Ling, Chen; Zhong, Li; Li, Mengxin; Su, Qin; He, Ran; Tang, Qiushi; Greiner, Dale L; Shultz, Leonard D; Brehm, Michael A; Flotte, Terence R; Mueller, Christian; Srivastava, Arun; Gao, Guangping

    2015-12-01

    Recombinant adeno-associated virus serotype 3B (rAAV3B) can transduce cultured human liver cancer cells and primary human hepatocytes efficiently. Serine (S)- and threonine (T)-directed capsid modifications further augment its transduction efficiency. Systemically delivered capsid-optimized rAAV3B vectors can specifically target cancer cells in a human liver cancer xenograft model, suggesting their potential use for human liver-directed gene therapy. Here, we compared transduction efficiencies of AAV3B and AAV8 vectors in cultured primary human hepatocytes and cancer cells as well as in human and mouse hepatocytes in a human liver xenograft NSG-PiZ mouse model. We also examined the safety and transduction efficacy of wild-type (WT) and capsid-optimized rAAV3B in the livers of nonhuman primates (NHPs). Intravenously delivered S663V+T492V (ST)-modified self-complementary (sc) AAV3B-EGFP vectors led to liver-targeted robust enhanced green fluorescence protein (EGFP) expression in NHPs without apparent hepatotoxicity. Intravenous injections of both WT and ST-modified rAAV3B.ST-rhCG vectors also generated stable super-physiological levels of rhesus chorionic gonadotropin (rhCG) in NHPs. The vector genome predominantly targeted the liver. Clinical chemistry and histopathology examinations showed no apparent vector-related toxicity. Our studies should be important and informative for clinical development of optimized AAV3B vectors for human liver-directed gene therapy.

  15. Heavy metal accumulation and signal transduction in herbaceous and woody plants: Paving the way for enhancing phytoremediation efficiency.

    PubMed

    Luo, Zhi-Bin; He, Jiali; Polle, Andrea; Rennenberg, Heinz

    2016-11-01

    Heavy metal (HM)-accumulating herbaceous and woody plants are employed for phytoremediation. To develop improved strategies for enhancing phytoremediation efficiency, knowledge of the microstructural, physiological and molecular responses underlying HM-accumulation is required. Here we review the progress in understanding the structural, physiological and molecular mechanisms underlying HM uptake, transport, sequestration and detoxification, as well as the regulation of these processes by signal transduction in response to HM exposure. The significance of genetic engineering for enhancing phytoremediation efficiency is also discussed. In herbaceous plants, HMs are taken up by roots and transported into the root cells via transmembrane carriers for nutritional ions. The HMs absorbed by root cells can be further translocated to the xylem vessels and unloaded into the xylem sap, thereby reaching the aerial parts of plants. HMs can be sequestered in the cell walls, vacuoles and the Golgi apparatuses. Plant roots initially perceive HM stress and trigger the signal transduction, thereby mediating changes at the molecular, physiological, and microstructural level. Signaling molecules such as phytohormones, reactive oxygen species (ROS) and nitric oxide (NO), modulate plant responses to HMs via differentially expressed genes, activation of the antioxidative system and coordinated cross talk among different signaling molecules. A number of genes participated in HM uptake, transport, sequestration and detoxification have been functionally characterized and transformed to target plants for enhancing phytoremediation efficiency. Fast growing woody plants hold an advantage over herbaceous plants for phytoremediation in terms of accumulation of high HM-amounts in their large biomass. Presumably, woody plants accumulate HMs using similar mechanisms as herbaceous counterparts, but the processes of HM accumulation and signal transduction can be more complex in woody plants

  16. Au-Ag@Au Hollow Nanostructure with Enhanced Chemical Stability and Improved Photothermal Transduction Efficiency for Cancer Treatment.

    PubMed

    Jiang, Tongtong; Song, Jiangluqi; Zhang, Wenting; Wang, Hao; Li, Xiaodong; Xia, Ruixiang; Zhu, Lixin; Xu, Xiaoliang

    2015-10-07

    Despite the fact that Au-Ag hollow nanoparticles (HNPs) have gained much attention as ablation agents for photothermal therapy, the instability of the Ag element limits their applications. Herein, excess Au atoms were deposited on the surface of a Au-Ag HNP by improving the reduction power of l-ascorbic acid (AA) and thereby preventing the reaction between HAuCl4 and the Ag element in the Au-Ag alloy nanostructure. Significantly, the obtained Au-Ag@Au HNPs show excellent chemical stability in an oxidative environment, together with remarkable increase in extinction peak intensity and obvious narrowing in peak width. Moreover, finite-difference time-domain (FDTD) was used to simulate the optical properties and electric field distribution of HNPs. The calculated results show that the proportion of absorption cross section in total extinction cross section increases with the improvement of Au content in HNP. As predicted by the theoretical calculation results, Au-Ag@Au nanocages (NCs) exhibit a photothermal transduction efficiency (η) as high as 36.5% at 808 nm, which is higher than that of Au-Ag NCs (31.2%). Irradiated by 808 nm laser at power densities of 1 W/cm(2), MCF-7 breast cancer cells incubated with PEGylated Au-Ag@Au NCs were seriously destroyed. Combined together, Au-Ag@Au HNPs with enhanced chemical stability and improved photothermal transduction efficiency show superior competitiveness as photothermal agents.

  17. Efficient transduction of cytotoxic and anti-HIV-1 genes by a gene-regulatable lentiviral vector.

    PubMed

    Shinoda, Yasuhiko; Hieda, Kuniko; Koyanagi, Yoshio; Suzuki, Youichi

    2009-10-01

    Lentiviral vectors modified from human immunodeficiency virus type 1 (HIV-1) offer a promising approach for gene therapy, facilitating transduction of genes into non-dividing cells both in vitro and in vivo. When transducing cytotoxic or anti-HIV genes, however, the vector must avoid self-inhibition by the transgene that can lead to a disruption in production of infectious virions. In this study, we constructed two HIV-1-based lentiviral vectors harboring the mifepristone-inducible gene expression unit in either the forward or the reverse orientation with respect to the direction of viral genomic RNA. The ability of these vectors to transduce cytotoxic and anti-HIV genes was evaluated. When human CD14 was used as a transgene, infectious lentiviral vectors were produced by both forward and reverse vector systems. CD14 expression was efficiently induced in cells transduced by both lentiviral vectors following treatment with mifepristone. However, a higher level of basal transgene expression was observed in the forward vector system in the absence of mifepristone. In contrast, high titers of infectious lentiviral vector containing the cytotoxic vesicular stomatitis virus M gene were successfully generated using the reverse vector, but not the forward vector. In addition, when a VPS4Bdominant negative mutant against HIV-1 budding was cloned into the reverse vector, significant amounts of lentiviral vector were obtained. Subsequent transduction of cells with the VPS4B mutant resulted in approximately 50% inhibition of HIV-1 production only in the presence of mifepristone. Our study thus demonstrates that incorporation of a mifepristone-regulatable gene expression unit in the reverse orientation makes significant advances toward development of a lentiviral vector that allows transduction of harmful genes.

  18. Efficient myogenic differentiation of human adipose-derived stem cells by the transduction of engineered MyoD protein

    SciTech Connect

    Sung, Min Sun; Mun, Ji-Young; Kwon, Ohsuk; Kwon, Ki-Sun; Oh, Doo-Byoung

    2013-07-19

    Highlights: •MyoD was engineered to contain protein transduction domain and endosome-disruptive INF7 peptide. •The engineered MyoD-IT showed efficient nuclear targeting through an endosomal escape by INF7 peptide. •By applying MyoD-IT, human adipose-derived stem cells (hASCs) were differentiated into myogenic cells. •hASCs differentiated by applying MyoD-IT fused to myotubes through co-culturing with mouse myoblasts. •Myogenic differentiation using MyoD-IT is a safe method without the concern of altering the genome. -- Abstract: Human adipose-derived stem cells (hASCs) have great potential as cell sources for the treatment of muscle disorders. To provide a safe method for the myogenic differentiation of hASCs, we engineered the MyoD protein, a key transcription factor for myogenesis. The engineered MyoD (MyoD-IT) was designed to contain the TAT protein transduction domain for cell penetration and the membrane-disrupting INF7 peptide, which is an improved version of the HA2 peptide derived from influenza. MyoD-IT showed greatly improved nuclear targeting ability through an efficient endosomal escape induced by the pH-sensitive membrane disruption of the INF7 peptide. By applying MyoD-IT to a culture, hASCs were efficiently differentiated into long spindle-shaped myogenic cells expressing myosin heavy chains. Moreover, these cells differentiated by an application of MyoD-IT fused to myotubes with high efficiency through co-culturing with mouse C2C12 myoblasts. Because internalized proteins can be degraded in cells without altering the genome, the myogenic differentiation of hASCs using MyoD-IT would be a safe and clinically applicable method.

  19. Uncovering signal transduction networks from high-throughput data by integer linear programming.

    PubMed

    Zhao, Xing-Ming; Wang, Rui-Sheng; Chen, Luonan; Aihara, Kazuyuki

    2008-05-01

    Signal transduction is an important process that transmits signals from the outside of a cell to the inside to mediate sophisticated biological responses. Effective computational models to unravel such a process by taking advantage of high-throughput genomic and proteomic data are needed to understand the essential mechanisms underlying the signaling pathways. In this article, we propose a novel method for uncovering signal transduction networks (STNs) by integrating protein interaction with gene expression data. Specifically, we formulate STN identification problem as an integer linear programming (ILP) model, which can be actually solved by a relaxed linear programming algorithm and is flexible for handling various prior information without any restriction on the network structures. The numerical results on yeast MAPK signaling pathways demonstrate that the proposed ILP model is able to uncover STNs or pathways in an efficient and accurate manner. In particular, the prediction results are found to be in high agreement with current biological knowledge and available information in literature. In addition, the proposed model is simple to be interpreted and easy to be implemented even for a large-scale system.

  20. Recombinant adeno-associated viral (rAAV) vectors mediate efficient gene transduction in cultured neonatal and adult microglia.

    PubMed

    Su, Wei; Kang, John; Sopher, Bryce; Gillespie, James; Aloi, Macarena S; Odom, Guy L; Hopkins, Stephanie; Case, Amanda; Wang, David B; Chamberlain, Jeffrey S; Garden, Gwenn A

    2016-01-01

    Microglia are a specialized population of myeloid cells that mediate CNS innate immune responses. Efforts to identify the cellular and molecular mechanisms that regulate microglia behaviors have been hampered by the lack of effective tools for manipulating gene expression. Cultured microglia are refractory to most chemical and electrical transfection methods, yielding little or no gene delivery and causing toxicity and/or inflammatory activation. Recombinant adeno-associated viral (rAAVs) vectors are non-enveloped, single-stranded DNA vectors commonly used to transduce many primary cell types and tissues. In this study, we evaluated the feasibility and efficiency of utilizing rAAV serotype 2 (rAAV2) to modulate gene expression in cultured microglia. rAAV2 yields high transduction and causes minimal toxicity or inflammatory response in both neonatal and adult microglia. To demonstrate that rAAV transduction can induce functional protein expression, we used rAAV2 expressing Cre recombinase to successfully excise a LoxP-flanked miR155 gene in cultured microglia. We further evaluated rAAV serotypes 5, 6, 8, and 9, and observed that all efficiently transduced cultured microglia to varying degrees of success and caused little or no alteration in inflammatory gene expression. These results provide strong encouragement for the application of rAAV-mediated gene expression in microglia for mechanistic and therapeutic purposes. Neonatal microglia are functionally distinct from adult microglia, although the majority of in vitro studies utilize rodent neonatal microglia cultures because of difficulties of culturing adult cells. In addition, cultured microglia are refractory to most methods for modifying gene expression. Here, we developed a novel protocol for culturing adult microglia and evaluated the feasibility and efficiency of utilizing Recombinant Adeno-Associated Virus (rAAV) to modulate gene expression in cultured microglia.

  1. Stochastic model-assisted development of efficient low-dose viral transduction in microfluidics.

    PubMed

    Luni, Camilla; Michielin, Federica; Barzon, Luisa; Calabrò, Vincenza; Elvassore, Nicola

    2013-02-19

    Adenoviruses are commonly used in vitro as gene transfer vectors in multiple applications. Nevertheless, issues such as low infection efficiency and toxicity effects on host cells have not been resolved yet. This work aims at developing a new versatile tool to enhance the expression of transduced genes while working at low viral doses in a sequential manner. We developed a microfluidic platform with automatically controlled sequential perfusion stages, which includes 10 independent channels. In addition, we built a stochastic mathematical model, accounting for the discrete nature of cells and viruses, to predict not only the percentage of infected cells, but also the associated infecting-virus distribution in the cell population. Microfluidic system and mathematical model were coupled to define an efficient experimental strategy. We used human foreskin fibroblasts, infected by replication-incompetent adenoviruses carrying EGFP gene, as the testing system. Cell characterization was performed through fluorescence microscopy, followed by image analysis. We explored the effect of different aspects: perfusion, multiplicity of infection, and temporal patterns of infection. We demonstrated feasibility of performing efficient viral transduction at low doses, by repeated pulses of cell-virus contact. This procedure also enhanced the exogenous gene expression in the sequential microfluidic infection system compared to a single infection at a higher, nontoxic, viral dose.

  2. Stochastic Model-Assisted Development of Efficient Low-Dose Viral Transduction in Microfluidics

    PubMed Central

    Luni, Camilla; Michielin, Federica; Barzon, Luisa; Calabrò, Vincenza; Elvassore, Nicola

    2013-01-01

    Adenoviruses are commonly used in vitro as gene transfer vectors in multiple applications. Nevertheless, issues such as low infection efficiency and toxicity effects on host cells have not been resolved yet. This work aims at developing a new versatile tool to enhance the expression of transduced genes while working at low viral doses in a sequential manner. We developed a microfluidic platform with automatically controlled sequential perfusion stages, which includes 10 independent channels. In addition, we built a stochastic mathematical model, accounting for the discrete nature of cells and viruses, to predict not only the percentage of infected cells, but also the associated infecting-virus distribution in the cell population. Microfluidic system and mathematical model were coupled to define an efficient experimental strategy. We used human foreskin fibroblasts, infected by replication-incompetent adenoviruses carrying EGFP gene, as the testing system. Cell characterization was performed through fluorescence microscopy, followed by image analysis. We explored the effect of different aspects: perfusion, multiplicity of infection, and temporal patterns of infection. We demonstrated feasibility of performing efficient viral transduction at low doses, by repeated pulses of cell-virus contact. This procedure also enhanced the exogenous gene expression in the sequential microfluidic infection system compared to a single infection at a higher, nontoxic, viral dose. PMID:23442972

  3. Z-shaped magnetostrictive patch for efficient transduction of a torsional wave mode in a cylindrical waveguide

    NASA Astrophysics Data System (ADS)

    Park, Chan Il; Cho, Seung Hyun; Kim, Yoon Young

    2006-10-01

    An earlier investigation [C. I. Park et al., Appl. Phys. Lett. 87, 224105 (2005)] using topology optimization showed that if V-shaped yokes are attached to magnetostrictive strips obliquely bonded on the surface of a cylindrical waveguide, the transduction efficiency of ultrasonic torsional waves can be substantially increased. However, some unwanted wave modes are also generated unless some damping treatment is applied. This investigation shows that if the strips are reconfigured as Z-shaped patches, the transduction efficiency gained by the optimization technique can be maintained without the generation of unwanted modes. By experiments using the Z-shaped patches, the transduction characteristics including the frequency characteristics of an ultrasonic torsional wave are also investigated.

  4. AAV8 capsid variable regions at the two-fold symmetry axis contribute to high liver transduction by mediating nuclear entry and capsid uncoating

    SciTech Connect

    Tenney, Rebeca M.; Bell, Christie L.; Wilson, James M.

    2014-04-15

    Adeno-associated virus serotype 8 (AAV8) is a promising vector for liver-directed gene therapy. Although efficient uncoating of viral capsids has been implicated in AAV8's robust liver transduction, much about the biology of AAV8 hepatotropism remains unclear. Our study investigated the structural basis of AAV8 liver transduction efficiency by constructing chimeric vector capsids containing sequences derived from AAV8 and AAV2 – a highly homologous yet poorly hepatotropic serotype. Engineered vectors containing capsid variable regions (VR) VII and IX from AAV8 in an AAV2 backbone mediated near AAV8-like transduction in mouse liver, with higher numbers of chimeric genomes detected in whole liver cells and isolated nuclei. Interestingly, chimeric capsids within liver nuclei also uncoated similarly to AAV8 by 6 weeks after administration, in contrast with AAV2, of which a significantly smaller proportion were uncoated. This study links specific AAV capsid regions to the transduction ability of a clinically relevant AAV serotype. - Highlights: • We construct chimeric vectors to identify determinants of AAV8 liver transduction. • An AAV2-based vector with 17 AAV8 residues exhibited high liver transduction in mice. • This vector also surpassed AAV2 in cell entry, nuclear entry and onset of expression. • Most chimeric vector particles were uncoated at 6 weeks, like AAV8 and unlike AAV2. • Chimera retained heparin binding and was antigenically distinct from AAV2 and AAV8.

  5. Efficient myogenic differentiation of human adipose-derived stem cells by the transduction of engineered MyoD protein.

    PubMed

    Sung, Min Sun; Mun, Ji-Young; Kwon, Ohsuk; Kwon, Ki-Sun; Oh, Doo-Byoung

    2013-07-19

    Human adipose-derived stem cells (hASCs) have great potential as cell sources for the treatment of muscle disorders. To provide a safe method for the myogenic differentiation of hASCs, we engineered the MyoD protein, a key transcription factor for myogenesis. The engineered MyoD (MyoD-IT) was designed to contain the TAT protein transduction domain for cell penetration and the membrane-disrupting INF7 peptide, which is an improved version of the HA2 peptide derived from influenza. MyoD-IT showed greatly improved nuclear targeting ability through an efficient endosomal escape induced by the pH-sensitive membrane disruption of the INF7 peptide. By applying MyoD-IT to a culture, hASCs were efficiently differentiated into long spindle-shaped myogenic cells expressing myosin heavy chains. Moreover, these cells differentiated by an application of MyoD-IT fused to myotubes with high efficiency through co-culturing with mouse C2C12 myoblasts. Because internalized proteins can be degraded in cells without altering the genome, the myogenic differentiation of hASCs using MyoD-IT would be a safe and clinically applicable method. Copyright © 2013 Elsevier Inc. All rights reserved.

  6. Interferometric Motion Detection in Atomic Layer 2D Nanostructures: Visualizing Signal Transduction Efficiency and Optimization Pathways

    NASA Astrophysics Data System (ADS)

    Wang, Zenghui; Feng, Philip X.-L.

    2016-07-01

    Atomic layer crystals are emerging building blocks for enabling new two-dimensional (2D) nanomechanical systems, whose motions can be coupled to other attractive physical properties in such 2D systems. Optical interferometry has been very effective in reading out the infinitesimal motions of these 2D structures and spatially resolving different modes. To quantitatively understand the detection efficiency and its dependence on the device parameters and interferometric conditions, here we present a systematic study of the intrinsic motion responsivity in 2D nanomechanical systems using a Fresnel-law-based model. We find that in monolayer to 14-layer structures, MoS2 offers the highest responsivity among graphene, h-BN, and MoS2 devices and for the three commonly used visible laser wavelengths (633, 532, and 405 nm). We also find that the vacuum gap resulting from the widely used 300 nm-oxide substrate in making 2D devices, fortunately, leads to close-to-optimal responsivity for a wide range of 2D flakes. Our results elucidate and graphically visualize the dependence of motion transduction responsivity upon 2D material type and number of layers, vacuum gap, oxide thickness, and detecting wavelength, thus providing design guidelines for constructing 2D nanomechanical systems with optimal optical motion readout.

  7. Interferometric Motion Detection in Atomic Layer 2D Nanostructures: Visualizing Signal Transduction Efficiency and Optimization Pathways.

    PubMed

    Wang, Zenghui; Feng, Philip X-L

    2016-07-28

    Atomic layer crystals are emerging building blocks for enabling new two-dimensional (2D) nanomechanical systems, whose motions can be coupled to other attractive physical properties in such 2D systems. Optical interferometry has been very effective in reading out the infinitesimal motions of these 2D structures and spatially resolving different modes. To quantitatively understand the detection efficiency and its dependence on the device parameters and interferometric conditions, here we present a systematic study of the intrinsic motion responsivity in 2D nanomechanical systems using a Fresnel-law-based model. We find that in monolayer to 14-layer structures, MoS2 offers the highest responsivity among graphene, h-BN, and MoS2 devices and for the three commonly used visible laser wavelengths (633, 532, and 405 nm). We also find that the vacuum gap resulting from the widely used 300 nm-oxide substrate in making 2D devices, fortunately, leads to close-to-optimal responsivity for a wide range of 2D flakes. Our results elucidate and graphically visualize the dependence of motion transduction responsivity upon 2D material type and number of layers, vacuum gap, oxide thickness, and detecting wavelength, thus providing design guidelines for constructing 2D nanomechanical systems with optimal optical motion readout.

  8. Combination of adenovirus and cross-linked low molecular weight PEI improves efficiency of gene transduction

    NASA Astrophysics Data System (ADS)

    Han, Jianfeng; Zhao, Dong; Zhong, Zhirong; Zhang, Zhirong; Gong, Tao; Sun, Xun

    2010-03-01

    Recombinant adenovirus (Ad)-mediated gene therapy is an exciting novel strategy in cancer treatment. However, poor infection efficiency with coxsackievirus and adenovirus receptor (CAR) down-regulated cancer cell lines is one of the major challenges for its practical and extensive application. As an alternative method of viral gene delivery, a non-viral carrier using cationic materials could compensate for the limitation of adenovirus. In our study, adenovectors were complexed with a new synthetic polymer PEI-DEG-bis-NPC (PDN) based on polyethylenimine (PEI), and then the properties of the vehicle were characterized by measurement of size distribution, zeta potential and transmission electron microscopy (TEM). Enhancement of gene transduction by Ad/PDN complexes was observed in both CAR-overexpressing cell lines (A549) and CAR-lacking cell lines (MDCK, CHO, LLC), as a result of facilitating binding and cell uptake of adenoviral particles by the cationic component. Ad/PDN complexes also promoted the inhibition of tumor growth in vivo and prolonged the survival time of tumor-bearing mice. These data suggest that a combination of viral and non-viral gene delivery methods may offer a new approach to successful cancer gene therapy.

  9. Interferometric Motion Detection in Atomic Layer 2D Nanostructures: Visualizing Signal Transduction Efficiency and Optimization Pathways

    PubMed Central

    Wang, Zenghui; Feng, Philip X.-L.

    2016-01-01

    Atomic layer crystals are emerging building blocks for enabling new two-dimensional (2D) nanomechanical systems, whose motions can be coupled to other attractive physical properties in such 2D systems. Optical interferometry has been very effective in reading out the infinitesimal motions of these 2D structures and spatially resolving different modes. To quantitatively understand the detection efficiency and its dependence on the device parameters and interferometric conditions, here we present a systematic study of the intrinsic motion responsivity in 2D nanomechanical systems using a Fresnel-law-based model. We find that in monolayer to 14-layer structures, MoS2 offers the highest responsivity among graphene, h-BN, and MoS2 devices and for the three commonly used visible laser wavelengths (633, 532, and 405 nm). We also find that the vacuum gap resulting from the widely used 300 nm-oxide substrate in making 2D devices, fortunately, leads to close-to-optimal responsivity for a wide range of 2D flakes. Our results elucidate and graphically visualize the dependence of motion transduction responsivity upon 2D material type and number of layers, vacuum gap, oxide thickness, and detecting wavelength, thus providing design guidelines for constructing 2D nanomechanical systems with optimal optical motion readout. PMID:27464908

  10. Recombinant adeno-associated virus-mediated high-efficiency, transient expression of the murine cationic amino acid transporter (ecotropic retroviral receptor) permits stable transduction of human HeLa cells by ecotropic retroviral vectors.

    PubMed Central

    Bertran, J; Miller, J L; Yang, Y; Fenimore-Justman, A; Rueda, F; Vanin, E F; Nienhuis, A W

    1996-01-01

    Adeno-associated virus has a broad host range, is nonpathogenic, and integrates into a preferred location on chromosome 19, features that have fostered development of recombinant adeno-associated viruses (rAAV) as gene transfer vectors for therapeutic applications. We have used an rAAV to transfer and express the murine cationic amino acid transporter which functions as the ecotropic retroviral receptor, thereby rendering human cells conditionally susceptible to infection by an ecotropic retroviral vector. The proportion of human HeLa cells expressing the receptor at 60 h varied as a function of the multiplicity of infection (MOI) with the rAAV. Cells expressing the ecotropic receptor were efficiently transduced with an ecotropic retroviral vector encoding a nucleus-localized form of beta-galactosidase. Cells coexpressing the ecotropic receptor and nucleus-localized beta-galactosidase were isolated by fluorescence-activated cell sorting, and cell lines were recovered by cloning at limiting dilution. After growth in culture, all clones contained the retroviral vector genome, but fewer than 10% (3 of 47) contained the rAAV genome and continued to express the ecotropic receptor. The ecotropic receptor coding sequences in the rAAV genome were under the control of a tetracycline-modulated promoter. In the presence of tetracycline, receptor expression was low and the proportion of cells transduced by the ecotropic retroviral vector was decreased. Modulation of receptor expression was achieved with both an episomal and an integrated form of the rAAV genome. These data establish that functional gene expression from an rAAV genome can occur transiently without genome integration. PMID:8794313

  11. Herpes simplex viral-vector design for efficient transduction of nonneuronal cells without cytotoxicity.

    PubMed

    Miyagawa, Yoshitaka; Marino, Pietro; Verlengia, Gianluca; Uchida, Hiroaki; Goins, William F; Yokota, Shinichiro; Geller, David A; Yoshida, Osamu; Mester, Joseph; Cohen, Justus B; Glorioso, Joseph C

    2015-03-31

    The design of highly defective herpes simplex virus (HSV) vectors for transgene expression in nonneuronal cells in the absence of toxic viral-gene activity has been elusive. Here, we report that elements of the latency locus protect a nonviral promoter against silencing in primary human cells in the absence of any viral-gene expression. We identified a CTCF motif cluster 5' to the latency promoter and a known long-term regulatory region as important elements for vigorous transgene expression from a vector that is functionally deleted for all five immediate-early genes and the 15-kb internal repeat region. We inserted a 16.5-kb expression cassette for full-length mouse dystrophin and report robust and durable expression in dystrophin-deficient muscle cells in vitro. Given the broad cell tropism of HSV, our design provides a nontoxic vector that can accommodate large transgene constructs for transduction of a wide variety of cells without vector integration, thereby filling an important void in the current arsenal of gene-therapy vectors.

  12. Herpes simplex viral-vector design for efficient transduction of nonneuronal cells without cytotoxicity

    PubMed Central

    Miyagawa, Yoshitaka; Marino, Pietro; Verlengia, Gianluca; Uchida, Hiroaki; Goins, William F.; Yokota, Shinichiro; Geller, David A.; Yoshida, Osamu; Mester, Joseph; Cohen, Justus B.; Glorioso, Joseph C.

    2015-01-01

    The design of highly defective herpes simplex virus (HSV) vectors for transgene expression in nonneuronal cells in the absence of toxic viral-gene activity has been elusive. Here, we report that elements of the latency locus protect a nonviral promoter against silencing in primary human cells in the absence of any viral-gene expression. We identified a CTCF motif cluster 5′ to the latency promoter and a known long-term regulatory region as important elements for vigorous transgene expression from a vector that is functionally deleted for all five immediate-early genes and the 15-kb internal repeat region. We inserted a 16.5-kb expression cassette for full-length mouse dystrophin and report robust and durable expression in dystrophin-deficient muscle cells in vitro. Given the broad cell tropism of HSV, our design provides a nontoxic vector that can accommodate large transgene constructs for transduction of a wide variety of cells without vector integration, thereby filling an important void in the current arsenal of gene-therapy vectors. PMID:25775541

  13. Identification and Validation of Small Molecules That Enhance Recombinant Adeno-associated Virus Transduction following High-Throughput Screens

    PubMed Central

    Nicolson, Sarah C.; Li, Chengwen; Hirsch, Matthew L.; Setola, Vincent

    2016-01-01

    ABSTRACT While the recent success of adeno-associated virus (AAV)-mediated gene therapy in clinical trials is promising, challenges still face the widespread applicability of recombinant AAV(rAAV). A major goal is to enhance the transduction efficiency of vectors in order to achieve therapeutic levels of gene expression at a vector dose that is below the immunological response threshold. In an attempt to identify novel compounds that enhance rAAV transduction, we performed two high-throughput screens comprising 2,396 compounds. We identified 13 compounds that were capable of enhancing transduction, of which 12 demonstrated vector-specific effects and 1 could also enhance vector-independent transgene expression. Many of these compounds had similar properties and could be categorized into five groups: epipodophyllotoxins (group 1), inducers of DNA damage (group 2), effectors of epigenetic modification (group 3), anthracyclines (group 4), and proteasome inhibitors (group 5). We optimized dosing for the identified compounds in several immortalized human cell lines as well as normal diploid cells. We found that the group 1 epipodophyllotoxins (teniposide and etoposide) consistently produced the greatest transduction enhancement. We also explored transduction enhancement among single-stranded, self-complementary, and fragment vectors and found that the compounds could impact fragmented rAAV2 transduction to an even greater extent than single-stranded vectors. In vivo analysis of rAAV2 and all of the clinically relevant compounds revealed that, consistent with our in vitro results, teniposide exhibited the greatest level of transduction enhancement. Finally, we explored the capability of teniposide to enhance transduction of fragment vectors in vivo using an AAV8 capsid that is known to exhibit robust liver tropism. Consistent with our in vitro results, teniposide coadministration greatly enhanced fragmented rAAV8 transduction at 48 h and 8 days. This study provides a

  14. Comparative Transduction Efficiency of AAV Vector Serotypes 1–6 in the Substantia Nigra and Striatum of the Primate Brain

    PubMed Central

    Markakis, Eleni A; Vives, Kenneth P; Bober, Jeremy; Leichtle, Stefan; Leranth, Csaba; Beecham, Jeff; Elsworth, John D; Roth, Robert H; Samulski, R Jude; Redmond, D Eugene

    2009-01-01

    Vectors derived from adeno-associated virus (AAV) are promising candidates for neural cell transduction in vivo because they are nonpathogenic and achieve long-term transduction in the central nervous system. AAV serotype 2 (AAV2) is the most widely used AAV vector in clinical trials based largely on its ability to transduce neural cells in the rodent and primate brain. Prior work in rodents suggests that other serotypes might be more efficient; however, a systematic evaluation of vector transduction efficiency has not yet been performed in the primate brain. In this study, AAV viral vectors of serotypes 1–6 with an enhanced green-fluorescent protein (GFP) reporter gene were generated at comparable titers, and injected in equal amounts into the brains of Chlorocebus sabaeus. Vector injections were placed in the substantia nigra (SN) and the caudate nucleus (CD). One month after injection, immunohistochemistry for GFP was performed and the total number of GFP+ cells was calculated using unbiased stereology. AAV5 was the most efficient vector, not only transducing significantly more cells than any other serotype, but also transducing both NeuN+ and glial-fibrillary-acidic protein positive (GFAP+) cells. These results suggest that AAV5 is a more effective vector than AAV2 at delivering potentially therapeutic transgenes to the nigrostriatal system of the primate brain. PMID:20010918

  15. High efficiency RCCI combustion

    NASA Astrophysics Data System (ADS)

    Splitter, Derek A.

    An experimental investigation of the pragmatic limits of Reactivity Controlled Compression Ignition (RCCI) engine efficiency was performed. The study utilized engine experiments combined with zero-dimensional modeling. Initially, simulations were used to suggest conditions of high engine efficiency with RCCI. Preliminary simulations suggested that high efficiency could be obtained by using a very dilute charge with a high compression ratio. Moreover, the preliminary simulations further suggested that with simultaneous 50% reductions in heat transfer and incomplete combustion, 60% gross thermal efficiency may be achievable with RCCI. Following the initial simulations, experiments to investigate the combustion process, fuel effects, and methods to reduce heat transfer and incomplete combustion reduction were conducted. The results demonstrated that the engine cycle and combustion process are linked, and if high efficiency is to be had, then the combustion event must be tailored to the initial cycle conditions. It was found that reductions to engine heat transfer are a key enabler to increasing engine efficiency. In addition, it was found that the piston oil jet gallery cooling in RCCI may be unnecessary, as it had a negative impact on efficiency. Without piston oil gallery cooling, it was found that RCCI was nearly adiabatic, achieving 95% of the theoretical maximum cycle efficiency (air standard Otto cycle efficiency).

  16. Chemical Modification with High Molecular Weight Polyethylene Glycol Reduces Transduction of Hepatocytes and Increases Efficacy of Intravenously Delivered Oncolytic Adenovirus

    PubMed Central

    Doronin, Konstantin; Shashkova, Elena V.; May, Shannon M.; Hofherr, Sean E.

    2009-01-01

    Abstract Oncolytic adenoviruses are anticancer agents that replicate within tumors and spread to uninfected tumor cells, amplifying the anticancer effect of initial transduction. We tested whether coating the viral particle with polyethylene glycol (PEG) could reduce transduction of hepatocytes and hepatotoxicity after systemic (intravenous) administration of oncolytic adenovirus serotype 5 (Ad5). Conjugating Ad5 with high molecular weight 20-kDa PEG but not with 5-kDa PEG reduced hepatocyte transduction and hepatotoxicity after intravenous injection. PEGylation with 20-kDa PEG was as efficient at detargeting adenovirus from Kupffer cells and hepatocytes as virus predosing and warfarin. Bioluminescence imaging of virus distribution in two xenograft tumor models in nude mice demonstrated that PEGylation with 20-kDa PEG reduced liver infection 19- to 90-fold. Tumor transduction levels were similar for vectors PEGylated with 20-kDa PEG and unPEGylated vectors. Anticancer efficacy after a single intravenous injection was retained at the level of unmodified vector in large established prostate carcinoma xenografts, resulting in complete elimination of tumors in all animals and long-term tumor-free survival. Anticancer efficacy after a single intravenous injection was increased in large established hepatocellular carcinoma xenografts, resulting in significant prolongation of survival as compared with unmodified vector. The increase in efficacy was comparable to that obtained with predosing and warfarin pretreatment, significantly extending the median of survival. Shielding adenovirus with 20-kDa PEG may be a useful approach to improve the therapeutic window of oncolytic adenovirus after systemic delivery to primary and metastatic tumor sites. PMID:19469693

  17. Experimental demonstration of high fidelity entanglement distribution over decoherence channels via qubit transduction.

    PubMed

    Lim, Hyang-Tag; Hong, Kang-Hee; Kim, Yoon-Ho

    2015-10-21

    Quantum coherence and entanglement, which are essential resources for quantum information, are often degraded and lost due to decoherence. Here, we report a proof-of-principle experimental demonstration of high fidelity entanglement distribution over decoherence channels via qubit transduction. By unitarily switching the initial qubit encoding to another, which is insensitive to particular forms of decoherence, we have demonstrated that it is possible to avoid the effect of decoherence completely. In particular, we demonstrate high-fidelity distribution of photonic polarization entanglement over quantum channels with two types of decoherence, amplitude damping and polarization-mode dispersion, via qubit transduction between polarization qubits and dual-rail qubits. These results represent a significant breakthrough in quantum communication over decoherence channels as the protocol is input-state independent, requires no ancillary photons and symmetries, and has near-unity success probability.

  18. An Interactive Macrophage Signal Transduction Map Facilitates Comparative Analyses of High-Throughput Data.

    PubMed

    Wentker, Pia; Eberhardt, Martin; Dreyer, Florian S; Bertrams, Wilhelm; Cantone, Martina; Griss, Kathrin; Schmeck, Bernd; Vera, Julio

    2017-03-01

    Macrophages (Mϕs) are key players in the coordination of the lifesaving or detrimental immune response against infections. The mechanistic understanding of the functional modulation of Mϕs by pathogens and pharmaceutical interventions at the signal transduction level is still far from complete. The complexity of pathways and their cross-talk benefits from holistic computational approaches. In the present study, we reconstructed a comprehensive, validated, and annotated map of signal transduction pathways in inflammatory Mϕs based on the current literature. In a second step, we selectively expanded this curated map with database knowledge. We provide both versions to the scientific community via a Web platform that is designed to facilitate exploration and analysis of high-throughput data. The platform comes preloaded with logarithmic fold changes from 44 data sets on Mϕ stimulation. We exploited three of these data sets-human primary Mϕs infected with the common lung pathogens Streptococcus pneumoniae, Legionella pneumophila, or Mycobacterium tuberculosis-in a case study to show how our map can be customized with expression data to pinpoint regulated subnetworks and druggable molecules. From the three infection scenarios, we extracted a regulatory core of 41 factors, including TNF, CCL5, CXCL10, IL-18, and IL-12 p40, and identified 140 drugs targeting 16 of them. Our approach promotes a comprehensive systems biology strategy for the exploitation of high-throughput data in the context of Mϕ signal transduction. In conclusion, we provide a set of tools to help scientists unravel details of Mϕ signaling. The interactive version of our Mϕ signal transduction map is accessible online at https://vcells.net/macrophage. Copyright © 2017 by The American Association of Immunologists, Inc.

  19. High efficiency incandescent lighting

    SciTech Connect

    Bermel, Peter; Ilic, Ognjen; Chan, Walker R.; Musabeyoglu, Ahmet; Cukierman, Aviv Ruben; Harradon, Michael Robert; Celanovic, Ivan; Soljacic, Marin

    2014-09-02

    Incandescent lighting structure. The structure includes a thermal emitter that can, but does not have to, include a first photonic crystal on its surface to tailor thermal emission coupled to, in a high-view-factor geometry, a second photonic filter selected to reflect infrared radiation back to the emitter while passing visible light. This structure is highly efficient as compared to standard incandescent light bulbs.

  20. High-speed odor transduction and pulse tracking by insect olfactory receptor neurons

    PubMed Central

    Szyszka, Paul; Gerkin, Richard C.; Galizia, C. Giovanni; Smith, Brian H.

    2014-01-01

    Sensory systems encode both the static quality of a stimulus (e.g., color or shape) and its kinetics (e.g., speed and direction). The limits with which stimulus kinetics can be resolved are well understood in vision, audition, and somatosensation. However, the maximum temporal resolution of olfactory systems has not been accurately determined. Here, we probe the limits of temporal resolution in insect olfaction by delivering high frequency odor pulses and measuring sensory responses in the antennae. We show that transduction times and pulse tracking capabilities of olfactory receptor neurons are faster than previously reported. Once an odorant arrives at the boundary layer of the antenna, odor transduction can occur within less than 2 ms and fluctuating odor stimuli can be resolved at frequencies more than 100 Hz. Thus, insect olfactory receptor neurons can track stimuli of very short duration, as occur when their antennae encounter narrow filaments in an odor plume. These results provide a new upper bound to the kinetics of odor tracking in insect olfactory receptor neurons and to the latency of initial transduction events in olfaction. PMID:25385618

  1. Differentially piezoresistive transduction of high-Q encapsulated SOI-MEMS resonators with sub-100 nm gaps.

    PubMed

    Li, Cheng-Syun; Li, Ming-Huang; Li, Sheng-Shian

    2015-01-01

    A differentially piezoresistive (piezo-R) readout proposed for single-crystal-silicon (SCS) microelectromechanical systems (MEMS) resonators is implemented in a foundrybased resonator platform, demonstrating effective feedthrough cancellation using just simple piezoresistors from the resonator supports while maximizing their capacitively transduced driving areas. The SCS resonators are fabricated by a CMOS foundry using an SOI-MEMS technology together with a polysilicon refill process. A high electromechanical coupling coefficient is attained by the use of 50-nm transducer gap spacing. Moreover, a vacuum package of the fabricated resonators is carried out through wafer-level bonding process. In this work, the corner supporting beams of the resonator serve not only mechanical supports but also piezoresistors for detecting the motional signal, hence substantially simplifying the overall resonator design to realize the piezo-R sensing. In addition, the fabricated resonators are capable of either capacitive sensing or piezo-R detection under the same capacitive drive. To mitigate feedthrough signals from parasitics, a differential measurement configuration of the piezo-R transduction is implemented in this work, featuring more than 30-dB improvement on the feedthrough level as compared with the single-ended piezo-R counterpart and purely capacitive sensing readout. Furthermore, the high-Q design of the mechanical supports is also investigated, offering Q more than 10 000 with efficient piezo-R transduction for MEMS resonators.

  2. High Efficiency Cell Development

    NASA Technical Reports Server (NTRS)

    Carbajal, B. G.

    1979-01-01

    The specific activity was to improve the tandem junction Cell (TJC) as a high efficiency solar cell. The TJC development was to be consistent with module assembly and should contribute to the overall goals of the Low-Cost Solar Array Project. During 1978, TJC efficiency improved from approximately 11 percent to approximately 16 percent (AMI). Photogenerated current densities in excess of 42 mA/sq cm were observed at AMO. Open circuit voltages as high as 0.615 V were measured at AMO. Fill factor was only 0.68 - 0.75 due to a nonoptimum metal contact design. A device model was conceived in which the solar cell is modelled as a transitor. There are virtually no interconnect or packaging factor systems and the TJC is compatible with all conventional module fabrication systems. A modification of the TJC, the Front Surface Field (FSF) cell, was also explored.

  3. High efficiency magnetic bearings

    NASA Technical Reports Server (NTRS)

    Studer, Philip A.; Jayaraman, Chaitanya P.; Anand, Davinder K.; Kirk, James A.

    1993-01-01

    Research activities concerning high efficiency permanent magnet plus electromagnet (PM/EM) pancake magnetic bearings at the University of Maryland are reported. A description of the construction and working of the magnetic bearing is provided. Next, parameters needed to describe the bearing are explained. Then, methods developed for the design and testing of magnetic bearings are summarized. Finally, a new magnetic bearing which allows active torque control in the off axes directions is discussed.

  4. Phenomenally High Transduction Air/gas Transducers for Practical Non-Contact Ultrasonic Applications

    NASA Astrophysics Data System (ADS)

    Bhardwaj, Mahesh C.

    2009-03-01

    Based on novel acoustic impedance matching layers and high coupling piezoelectric materials this paper describes exceptionally high air/gas transduction ultrasonic transducers. By providing applications oriented performance of these transducers we also usher in the era of much desired Non-Contact Ultrasound (NCU) testing and analysis of a wide range of materials including early stage formation of materials such as uncured composite prepregs, green ceramics and powder metals, plastics, elastomers, porous, hygroscopic, chemically bonded and other materials. Besides quality control, ultimately NCU offers timely opportunities for cost-effective materials production, energy savings, and environment protection.

  5. High Efficiency, Clean Combustion

    SciTech Connect

    Donald Stanton

    2010-03-31

    Energy use in trucks has been increasing at a faster rate than that of automobiles within the U.S. transportation sector. According to the Energy Information Administration (EIA) Annual Energy Outlook (AEO), a 23% increase in fuel consumption for the U.S. heavy duty truck segment is expected between 2009 to 2020. The heavy duty vehicle oil consumption is projected to grow between 2009 and 2050 while light duty vehicle (LDV) fuel consumption will eventually experience a decrease. By 2050, the oil consumption rate by LDVs is anticipated to decrease below 2009 levels due to CAFE standards and biofuel use. In contrast, the heavy duty oil consumption rate is anticipated to double. The increasing trend in oil consumption for heavy trucks is linked to the vitality, security, and growth of the U.S. economy. An essential part of a stable and vibrant U.S. economy is a productive U.S. trucking industry. Studies have shown that the U.S. gross domestic product (GDP) is strongly correlated to freight transport. Over 90% of all U.S. freight tonnage is transported by diesel power and over 75% is transported by trucks. Given the vital role that the trucking industry plays in the economy, improving the efficiency of the transportation of goods was a central focus of the Cummins High Efficient Clean Combustion (HECC) program. In a commercial vehicle, the diesel engine remains the largest source of fuel efficiency loss, but remains the greatest opportunity for fuel efficiency improvements. In addition to reducing oil consumption and the dependency on foreign oil, this project will mitigate the impact on the environment by meeting US EPA 2010 emissions regulations. Innovation is a key element in sustaining a U.S. trucking industry that is competitive in global markets. Unlike passenger vehicles, the trucking industry cannot simply downsize the vehicle and still transport the freight with improved efficiency. The truck manufacturing and supporting industries are faced with numerous

  6. Superstructure high efficiency photovoltaics

    NASA Technical Reports Server (NTRS)

    Wagner, M.; So, L. C.; Leburton, J. P.

    1987-01-01

    A novel class of photovoltaic cascade structures is introduced which features multijunction upper subcells. These superstructure high efficiency photovoltaics (SHEP's) exhibit enhanced upper subcell spectral response because of the additional junctions which serve to reduce bulk recombination losses by decreasing the mean collection distance for photogenerated minority carriers. Two possible electrical configurations were studied and compared: a three-terminal scheme that allows both subcells to be operated at their individual maximum power points and a two-terminal configuration with an intercell ohmic contact for series interconnection. The three-terminal devices were found to be superior both in terms of beginning-of-life expectancy and radiation tolerance. Realistic simulations of three-terminal AlGaAs/GaAs SHEP's show that one sun AMO efficiencies in excess of 26 percent are possible.

  7. The effect of surface modification of adenovirus with an arginine-grafted bioreducible polymer on transduction efficiency and immunogenicity in cancer gene therapy.

    PubMed

    Kim, Pyung-Hwan; Kim, Tae-Il; Yockman, James W; Kim, Sung Wan; Yun, Chae-Ok

    2010-03-01

    Adenoviral vectors offer many advantages for cancer gene therapy, including high transduction efficiency, but safety concerns related to severe immunogenicity and other side effects have led to careful reconsideration of their use in human clinical trials. To overcome these issues, a strategy of generating hybrid vectors that combine viral and non-viral elements as more intelligent gene carriers has been employed. Here, we coated adenovirus (Ad) with an arginine-grafted bioreducible polymer (ABP) via electrostatic interaction. We examined the effect of ABP-coated Ad complex at various ABP molecules/Ad particle ratios. Enhanced transduction efficiency was observed in cells treated with cationic ABP polymer-coated Ad complex compared to naked Ad. We also examined the coating of Ad with ABP polymers at the optimal polymer ratio using dynamic light scattering and transmission electron microscopy. In both high and low coxsackie virus and adenovirus receptor (CAR)-expressing cells, ABP-coated Ad complex produced higher levels of transgene expression than cationic polymer 25K PEI. Notably, high cytotoxicity was observed with 25K PEI-coated Ad complex treatment, but not with ABP-coated Ad complex treatment. In addition, ABP-coated Ad complex was not significantly inhibited by serum, in contrast to naked Ad. Moreover, ABP-coated Ad complex significantly reduced the innate immune response relative to naked Ad, as assessed by interleukin-6 (IL-6) cytokine release from macrophage cells. Overall, our studies demonstrate that Ad complex formed with ABP cationic polymer may improve the efficiency of Ad and be a promising tool for cancer gene therapy. (c) 2009 Elsevier Ltd. All rights reserved.

  8. High efficiency multifrequency feed

    NASA Technical Reports Server (NTRS)

    Ajioka, J. S.; Tsuda, G. I.; Leeper, W. A. (Inventor)

    1974-01-01

    Antenna systems and particularly compact and simple antenna feeds which can transmit and receive simultaneously in at least three frequency bands, each with high efficiency and polarization diversity are described. The feed system is applicable for frequency bands having nominal frequency bands with the ratio 1:4:6. By way of example, satellite communications telemetry bands operate in frequency bands 0.8 - 1.0 GHz, 3.7 - 4.2 GHz and 5.9 - 6.4 GHz. In addition, the antenna system of the invention has monopulse capability for reception with circular or diverse polarization at frequency band 1.

  9. High efficiency photoionization detector

    DOEpatents

    Anderson, D.F.

    1984-01-31

    A high efficiency photoionization detector is described using tetraaminoethylenes in a gaseous state having a low ionization potential and a relative photoionization cross section which closely matches the emission spectrum of xenon gas. Imaging proportional counters are also disclosed using the novel photoionization detector of the invention. The compound of greatest interest is TMAE which comprises tetrakis(dimethylamino)ethylene which has a measured ionization potential of 5.36 [+-] 0.02 eV, and a vapor pressure of 0.35 torr at 20 C. 6 figs.

  10. High efficiency photoionization detector

    DOEpatents

    Anderson, David F.

    1984-01-01

    A high efficiency photoionization detector using tetraaminoethylenes in a gaseous state having a low ionization potential and a relative photoionization cross section which closely matches the emission spectrum of xenon gas. Imaging proportional counters are also disclosed using the novel photoionization detector of the invention. The compound of greatest interest is TMAE which comprises tetrakis(dimethylamino)ethylene which has a measured ionization potential of 5.36.+-.0.02 eV, and a vapor pressure of 0.35 torr at 20.degree. C.

  11. Efficient gene delivery and selective transduction of astrocytes in the mammalian brain using viral vectors

    PubMed Central

    Merienne, Nicolas; Douce, Juliette Le; Faivre, Emilie; Déglon, Nicole; Bonvento, Gilles

    2013-01-01

    Astrocytes are now considered as key players in brain information processing because of their newly discovered roles in synapse formation and plasticity, energy metabolism and blood flow regulation. However, our understanding of astrocyte function is still fragmented compared to other brain cell types. A better appreciation of the biology of astrocytes requires the development of tools to generate animal models in which astrocyte-specific proteins and pathways can be manipulated. In addition, it is becoming increasingly evident that astrocytes are also important players in many neurological disorders. Targeted modulation of protein expression in astrocytes would be critical for the development of new therapeutic strategies. Gene transfer is valuable to target a subpopulation of cells and explore their function in experimental models. In particular, viral-mediated gene transfer provides a rapid, highly flexible and cost-effective, in vivo paradigm to study the impact of genes of interest during central nervous system development or in adult animals. We will review the different strategies that led to the recent development of efficient viral vectors that can be successfully used to selectively transduce astrocytes in the mammalian brain. PMID:23847471

  12. Better Targeting, Better Efficiency for Wide-Scale Neuronal Transduction with the Synapsin Promoter and AAV-PHP.B

    PubMed Central

    Jackson, Kasey L.; Dayton, Robert D.; Deverman, Benjamin E.; Klein, Ronald L.

    2016-01-01

    Widespread genetic modification of cells in the central nervous system (CNS) with a viral vector has become possible and increasingly more efficient. We previously applied an AAV9 vector with the cytomegalovirus/chicken beta-actin (CBA) hybrid promoter and achieved wide-scale CNS transduction in neonatal and adult rats. However, this method transduces a variety of tissues in addition to the CNS. Thus we studied intravenous AAV9 gene transfer with a synapsin promoter to better target the neurons. We noted in systematic comparisons that the synapsin promoter drives lower level expression than does the CBA promoter. The engineered adeno-associated virus (AAV)-PHP.B serotype was compared with AAV9, and AAV-PHP.B did enhance the efficiency of expression. Combining the synapsin promoter with AAV-PHP.B could therefore be advantageous in terms of combining two refinements of targeting and efficiency. Wide-scale expression was used to model a disease with widespread pathology. Vectors encoding the amyotrophic lateral sclerosis (ALS)-related protein transactive response DNA-binding protein, 43 kDa (TDP-43) with the synapsin promoter and AAV-PHP.B were used for efficient CNS-targeted TDP-43 expression. Intracerebroventricular injections were also explored to limit TDP-43 expression to the CNS. The neuron-selective promoter and the AAV-PHP.B enhanced gene transfer and ALS disease modeling in adult rats. PMID:27867348

  13. Better Targeting, Better Efficiency for Wide-Scale Neuronal Transduction with the Synapsin Promoter and AAV-PHP.B.

    PubMed

    Jackson, Kasey L; Dayton, Robert D; Deverman, Benjamin E; Klein, Ronald L

    2016-01-01

    Widespread genetic modification of cells in the central nervous system (CNS) with a viral vector has become possible and increasingly more efficient. We previously applied an AAV9 vector with the cytomegalovirus/chicken beta-actin (CBA) hybrid promoter and achieved wide-scale CNS transduction in neonatal and adult rats. However, this method transduces a variety of tissues in addition to the CNS. Thus we studied intravenous AAV9 gene transfer with a synapsin promoter to better target the neurons. We noted in systematic comparisons that the synapsin promoter drives lower level expression than does the CBA promoter. The engineered adeno-associated virus (AAV)-PHP.B serotype was compared with AAV9, and AAV-PHP.B did enhance the efficiency of expression. Combining the synapsin promoter with AAV-PHP.B could therefore be advantageous in terms of combining two refinements of targeting and efficiency. Wide-scale expression was used to model a disease with widespread pathology. Vectors encoding the amyotrophic lateral sclerosis (ALS)-related protein transactive response DNA-binding protein, 43 kDa (TDP-43) with the synapsin promoter and AAV-PHP.B were used for efficient CNS-targeted TDP-43 expression. Intracerebroventricular injections were also explored to limit TDP-43 expression to the CNS. The neuron-selective promoter and the AAV-PHP.B enhanced gene transfer and ALS disease modeling in adult rats.

  14. HIGH EFFICIENCY SYNGAS GENERATION

    SciTech Connect

    Robert J. Copeland; Yevgenia Gershanovich; Brian Windecker

    2005-02-01

    This project investigated an efficient and low cost method of auto-thermally reforming natural gas to hydrogen and carbon monoxide. Reforming is the highest cost step in producing products such as methanol and Fisher Tropsch liquids (i.e., gas to liquids); and reducing the cost of reforming is the key to reducing the cost of these products. Steam reforming is expensive because of the high cost of the high nickel alloy reforming tubes (i.e., indirectly fired reforming tubes). Conventional auto-thermal or Partial Oxidation (POX) reforming minimizes the size and cost of the reformers and provides a near optimum mixture of CO and hydrogen. However POX requires pure oxygen, which consumes power and significantly increases the cost to reforming. Our high efficiency process extracts oxygen from low-pressure air with novel oxygen sorbent and transfers the oxygen to a nickel-catalyzed reformer. The syngas is generated at process pressure (typically 20 to 40 bar) without nitrogen dilution and has a 1CO to 2H{sub 2} ratio that is near optimum for the subsequent production of Fisher-Tropsch liquid to liquids and other chemicals (i.e., Gas to Liquids, GTL). Our high process efficiency comes from the way we transfer the oxygen into the reformer. All of the components of the process, except for the oxygen sorbent, are commonly used in commercial practice. A process based on a longlived, regenerable, oxygen transfer sorbent could substantially reduce the cost of natural gas reforming to syngas. Lower cost syngas (CO + 2H{sub 2}) that is the feedstock for GTL would reduce the cost of GTL and for other commercial applications (e.g., methanol, other organic chemicals). The vast gas resources of Alaska's North Slope (ANS) offer more than 22 Tcf of gas and GTL production in this application alone, and could account for as much as 300,000 to 700,000 bpd for 20 to 30+ years. We developed a new sorbent, which is an essential part of the High Efficiency Oxygen Process (HOP). We tested the

  15. High-efficiency CARM

    SciTech Connect

    Bratman, V.L.; Kol`chugin, B.D.; Samsonov, S.V.; Volkov, A.B.

    1995-12-31

    The Cyclotron Autoresonance Maser (CARM) is a well-known variety of FEMs. Unlike the ubitron in which electrons move in a periodical undulator field, in the CARM the particles move along helical trajectories in a uniform magnetic field. Since it is much simpler to generate strong homogeneous magnetic fields than periodical ones for a relatively low electron energy ({Brit_pounds}{le}1-3 MeV) the period of particles` trajectories in the CARM can be sufficiently smaller than in the undulator in which, moreover, the field decreases rapidly in the transverse direction. In spite of this evident advantage, the number of papers on CARM is an order less than on ubitron, which is apparently caused by the low (not more than 10 %) CARM efficiency in experiments. At the same time, ubitrons operating in two rather complicated regimes-trapping and adiabatic deceleration of particles and combined undulator and reversed guiding fields - yielded efficiencies of 34 % and 27 %, respectively. The aim of this work is to demonstrate that high efficiency can be reached even for a simplest version of the CARM. In order to reduce sensitivity to an axial velocity spread of particles, a short interaction length where electrons underwent only 4-5 cyclotron oscillations was used in this work. Like experiments, a narrow anode outlet of a field-emission electron gun cut out the {open_quotes}most rectilinear{close_quotes} near-axis part of the electron beam. Additionally, magnetic field of a small correcting coil compensated spurious electron oscillations pumped by the anode aperture. A kicker in the form of a sloping to the axis frame with current provided a control value of rotary velocity at a small additional velocity spread. A simple cavity consisting of a cylindrical waveguide section restricted by a cut-off waveguide on the cathode side and by a Bragg reflector on the collector side was used as the CARM-oscillator microwave system.

  16. Evaluation of ADA gene expression and transduction efficiency in ADA/SCID patients undergoing gene therapy.

    PubMed

    Carlucci, F; Tabucchi, A; Aiuti, A; Rosi, F; Floccari, F; Pagani, R; Marinello, E

    2004-10-01

    A capillary electrophoresis (CE) method was developed for ADA/SCID diagnosis and monitoring of enzyme replacement therapy, as well as for exploring the transfection efficiency for different retroviral vectors in gene therapy.

  17. A rapid and efficient polyethylenimine-based transfection method to prepare lentiviral or retroviral vectors: useful for making iPS cells and transduction of primary cells.

    PubMed

    Yang, Shaozhe; Shi, Haijun; Chu, Xinran; Zhou, Xiaoling; Sun, Pingnan

    2016-09-01

    To improve the efficiency, reproducibility and consistency of the PEI-based transfection method that is often used in preparation of recombinant lentiviral or retroviral vectors. The contributions to transfection efficiency of multi-factors including concentration of PEI or DNA, dilution buffer for PEI/DNA, manner to prepare PEI/DNA complexes, influence of serum, incubation time for PEI/DNA complexes, and transfection time were studied. Gentle mixing during the preparation of PEI/DNA transfection complexes is critical for a high transfection efficiency. PEI could be stored at room temperature or 4 °C, and most importantly, multigelation should be avoided. The transfection efficiency of the PEI-based new method in different types of cells, such as 293T, Cos-7, HeLa, HepG2, Hep3B, Huh7 and L02, was also higher than that of the previous method. After optimization, the titer of our lentiviral system or retroviral system produced by PEI-based new method was about 10- or 3-times greater than that produced by PEI-based previous method, respectively. We provide a rapid and efficient PEI-based method for preparation of recombinant lentiviral or retroviral vectors which is useful for making iPS cells as well as transduction of primary cell cultures.

  18. Efficient transduction and optogenetic stimulation of retinal bipolar cells by a synthetic adeno-associated virus capsid and promoter

    PubMed Central

    Cronin, Therese; Vandenberghe, Luk H; Hantz, Péter; Juttner, Josephine; Reimann, Andreas; Kacsó, Ágota–Enikő; Huckfeldt, Rachel M; Busskamp, Volker; Kohler, Hubertus; Lagali, Pamela S; Roska, Botond; Bennett, Jean

    2014-01-01

    In this report, we describe the development of a modified adeno-associated virus (AAV) capsid and promoter for transduction of retinal ON-bipolar cells. The bipolar cells, which are post-synaptic to the photoreceptors, are important retinal targets for both basic and preclinical research. In particular, a therapeutic strategy under investigation for advanced forms of blindness involves using optogenetic molecules to render ON-bipolar cells light-sensitive. Currently, delivery of adequate levels of gene expression is a limiting step for this approach. The synthetic AAV capsid and promoter described here achieves high level of optogenetic transgene expression in ON-bipolar cells. This evokes high-frequency (∼100 Hz) spiking responses in ganglion cells of previously blind, rd1, mice. Our vector is a promising vehicle for further development toward potential clinical use. PMID:25092770

  19. High Efficiency Integrated Package

    SciTech Connect

    Ibbetson, James

    2013-09-15

    Solid-state lighting based on LEDs has emerged as a superior alternative to inefficient conventional lighting, particularly incandescent. LED lighting can lead to 80 percent energy savings; can last 50,000 hours – 2-50 times longer than most bulbs; and contains no toxic lead or mercury. However, to enable mass adoption, particularly at the consumer level, the cost of LED luminaires must be reduced by an order of magnitude while achieving superior efficiency, light quality and lifetime. To become viable, energy-efficient replacement solutions must deliver system efficacies of ≥ 100 lumens per watt (LPW) with excellent color rendering (CRI > 85) at a cost that enables payback cycles of two years or less for commercial applications. This development will enable significant site energy savings as it targets commercial and retail lighting applications that are most sensitive to the lifetime operating costs with their extended operating hours per day. If costs are reduced substantially, dramatic energy savings can be realized by replacing incandescent lighting in the residential market as well. In light of these challenges, Cree proposed to develop a multi-chip integrated LED package with an output of > 1000 lumens of warm white light operating at an efficacy of at least 128 LPW with a CRI > 85. This product will serve as the light engine for replacement lamps and luminaires. At the end of the proposed program, this integrated package was to be used in a proof-of-concept lamp prototype to demonstrate the component’s viability in a common form factor. During this project Cree SBTC developed an efficient, compact warm-white LED package with an integrated remote color down-converter. Via a combination of intensive optical, electrical, and thermal optimization, a package design was obtained that met nearly all project goals. This package emitted 1295 lm under instant-on, room-temperature testing conditions, with an efficacy of 128.4 lm/W at a color temperature of ~2873

  20. Efficient Transduction of Human and Rhesus Macaque Primary T Cells by a Modified Human Immunodeficiency Virus Type 1-Based Lentiviral Vector.

    PubMed

    He, Huan; Xue, Jing; Wang, Weiming; Liu, Lihong; Ye, Chaobaihui; Cong, Zhe; Kimata, Jason T; Qin, Chuan; Zhou, Paul

    2017-03-01

    Human immunodeficiency virus type 1 (HIV-1)-based lentiviral vectors efficiently transduce genes to human, but not rhesus, primary T cells and hematopoietic stem cells (HSCs). The poor transduction of HIV-1 vectors to rhesus cells is mainly due to species-specific restriction factors such as rhesus TRIM5α. Previously, several strategies to modify HIV-1 vectors were developed to overcome rhesus TRIM5α restriction. While the modified HIV-1 vectors efficiently transduce rhesus HSCs, they remain suboptimal for rhesus primary T cells. Recently, HIV-1 variants that encode combinations of LNEIE mutations in capsid (CA) protein and SIVmac239 Vif were found to replicate efficiently in rhesus primary T cells. Thus, the present study tested whether HIV-1 vectors packaged by a packaging construct containing these CA substitutions could efficiently transduce both human and rhesus primary CD4 T cells. To accomplish this, LNEIE mutations were made in the packaging construct CEMΔ8.9, and recombinant HIV-1 vectors packaged by Δ8.9 WT or Δ8.9 LNEIE were generated. Transduction rates, CA stability, and vector integration in CEMss-CCR5 and CEMss-CCR5-rhTRIM5α/green fluorescent protein cells, as well as transduction rates in human and rhesus primary CD4 T cells by Δ8.9 WT or Δ8.9 LNEIE-packaged HIV-1 vectors, were compared. Finally, the influence of rhesus TRIM5α variations in transduction rates to primary CD4 T cells from a cohort of 37 Chinese rhesus macaques was studied. While it maintains efficient transduction for human T-cell line and primary CD4 T cells, Δ8.9 LNEIE-packaged HIV-1 vector overcomes rhesus TRIM5α-mediated CA degradation, resulting in significantly higher transduction efficiency of rhesus primary CD4 T cells than Δ8.9 WT-packaged HIV-1 vector. Rhesus TRIM5α variations strongly influence transduction efficiency of rhesus primary CD4 T cells by both Δ8.9 WT or Δ8.9 LNEIE-packaged HIV-1 vectors. Thus, it is concluded that Δ8.9 LNEIE-packaged HIV-1

  1. Modified HIV-1 based lentiviral vectors have an effect on viral transduction efficiency and gene expression in vitro and in vivo.

    PubMed

    Park, F; Kay, M A

    2001-09-01

    Gene transfer using lentiviral vectors has been recently shown to be enhanced with cis-acting elements in a cell-type-dependent manner in vivo. For this reason, the study reported here was designed to modify lentiviral vectors that express lacZ, human factor IX (FIX), or human alpha1-anti-trypsin (AAT) to study the effect of different cis DNA elements on transduction efficiencies. We found that incorporation of the central polypurine tract sequence (cppt) increased transduction efficiency in vitro while increasing the transduction of non-cell-cycling hepatocytes in vivo. C57Bl/6 scid mice that were administered lentiviral vectors devoid of the cppt (2 x 10(8) transducing units (T.U.)/mouse) had 81% of their lacZ-transduced hepatocytes colabeled with the cell cycle marker 5'-bromo-2'-deoxyuridine (BrdU). In contrast, inclusion of the cppt reduced the colabeling in mouse hepatocytes by 50%. Further modifications in the lentiviral vectors were performed to enhance viral titer and gene expression. We found that the inclusion of a matrix attachment region (MAR) from immunoglobulin-kappa (Igkappa) significantly increased the transduction efficiency, as measured by transgene protein expression and proviral DNA copy number, compared with vectors without Igkappa MAR. In vitro studies using human hepatoma cells demonstrated a significant increase (two- to fourfold) in human AAT and human FIX production when the Igkappa MAR was incorporated. In vivo transduction of partially hepatectomized C57Bl/6 mice given an optimized lentiviral vector containing the cppt and Igkappa MAR (2 x 10(8) T.U./mouse) resulted in sustained therapeutic levels of serum FIX (approximately 65 ng/ml). Our study demonstrates the importance of cis-acting elements to enhancing the transduction ability of lentiviral vectors and the expression of vector transgenes.

  2. A bead-based western for high-throughput cellular signal transduction analyses

    PubMed Central

    Treindl, Fridolin; Ruprecht, Benjamin; Beiter, Yvonne; Schultz, Silke; Döttinger, Anette; Staebler, Annette; Joos, Thomas O.; Kling, Simon; Poetz, Oliver; Fehm, Tanja; Neubauer, Hans; Kuster, Bernhard; Templin, Markus F.

    2016-01-01

    Dissecting cellular signalling requires the analysis of large number of proteins. The DigiWest approach we describe here transfers the western blot to a bead-based microarray platform. By combining gel-based protein separation with immobilization on microspheres, hundreds of replicas of the initial blot are created, thus enabling the comprehensive analysis of limited material, such as cells collected by laser capture microdissection, and extending traditional western blotting to reach proteomic scales. The combination of molecular weight resolution, sensitivity and signal linearity on an automated platform enables the rapid quantification of hundreds of specific proteins and protein modifications in complex samples. This high-throughput western blot approach allowed us to identify and characterize alterations in cellular signal transduction that occur during the development of resistance to the kinase inhibitor Lapatinib, revealing major changes in the activation state of Ephrin-mediated signalling and a central role for p53-controlled processes. PMID:27659302

  3. Adeno-Associated Viral Vectors Based on Serotype 3b Use Components of the Fibroblast Growth Factor Receptor Signaling Complex for Efficient Transduction

    PubMed Central

    Messina, Emily L.; Nienaber, Jeffrey; Daneshmand, Mani; Villamizar, Nestor; Samulski, Jude; Milano, Carmelo

    2012-01-01

    Abstract Adeno-associated virus type 3b (AAV3b) has been largely ignored by gene therapists because of the inability of vectors based on this serotype to transduce target tissues efficiently. Here we describe a phenomenon unique to AAV3b in that vectors based on this serotype mediate enhanced transduction in the presence of heparin. Among the many biological functions attributed to heparin, its interaction with, and ability to regulate, several growth factors (GFs) and growth factor receptors (GFRs) has been well characterized. Using GFR-overexpressing cell lines, soluble GFs and heparins, as well as specific GFR inhibitors, we have demonstrated a requirement for fibroblast growth factor receptor-2 (FGFR2) and FGF1 in the heparin-mediated augmentation of AAV3b vector transduction. In contrast to AAV2, we establish that heparin can be used as an adjunct with AAV3b to further increase transduction in a variety of cells and target tissues, additionally suggesting that AAV3b may be an attractive viral vector for clinical use during procedures in which heparin is used. In summary, AAV3b exhibits FGFR2-dependent, markedly enhanced transduction efficiency in the presence of heparin and FGFs, which could make it a useful vector for gene therapy in a variety of human diseases. PMID:22680698

  4. Adeno-associated viral vectors based on serotype 3b use components of the fibroblast growth factor receptor signaling complex for efficient transduction.

    PubMed

    Messina, Emily L; Nienaber, Jeffrey; Daneshmand, Mani; Villamizar, Nestor; Samulski, Jude; Milano, Carmelo; Bowles, Dawn E

    2012-10-01

    Adeno-associated virus type 3b (AAV3b) has been largely ignored by gene therapists because of the inability of vectors based on this serotype to transduce target tissues efficiently. Here we describe a phenomenon unique to AAV3b in that vectors based on this serotype mediate enhanced transduction in the presence of heparin. Among the many biological functions attributed to heparin, its interaction with, and ability to regulate, several growth factors (GFs) and growth factor receptors (GFRs) has been well characterized. Using GFR-overexpressing cell lines, soluble GFs and heparins, as well as specific GFR inhibitors, we have demonstrated a requirement for fibroblast growth factor receptor-2 (FGFR2) and FGF1 in the heparin-mediated augmentation of AAV3b vector transduction. In contrast to AAV2, we establish that heparin can be used as an adjunct with AAV3b to further increase transduction in a variety of cells and target tissues, additionally suggesting that AAV3b may be an attractive viral vector for clinical use during procedures in which heparin is used. In summary, AAV3b exhibits FGFR2-dependent, markedly enhanced transduction efficiency in the presence of heparin and FGFs, which could make it a useful vector for gene therapy in a variety of human diseases.

  5. Efficient signal transduction by a chimeric yeast-mammalian G protein alpha subunit Gpa1-Gsalpha covalently fused to the yeast receptor Ste2.

    PubMed Central

    Medici, R; Bianchi, E; Di Segni, G; Tocchini-Valentini, G P

    1997-01-01

    Saccharomyces cerevisiae uses G protein-coupled receptors for signal transduction. We show that a fusion protein between the alpha-factor receptor (Ste2) and the Galpha subunit (Gpa1) transduces the signal efficiently in yeast cells devoid of the endogeneous STE2 and GPA1 genes. To evaluate the function of different domains of Galpha, a chimera between the N-terminal region of yeast Gpa1 and the C-terminal region of rat Gsalpha has been constructed. This chimeric Gpa1-Gsalpha is capable of restoring viability to haploid gpa1Delta cells, but signal transduction is prevented. This is consistent with evidence showing that the C-terminus of the homologous Galpha is required for receptor-G protein recognition. Surprisingly, a fusion protein between Ste2 and Gpa1-Gsalpha is able to transduce the signal efficiently. It appears, therefore, that the C-terminus of Galpha is mainly responsible for bringing the G protein into the close proximity of the receptor's intracellular domains, thus ensuring efficient coupling, rather than having a particular role in transmitting the signal. To confirm this conclusion, we show that two proteins interacting with each other (such as Snf1 and Snf4, or Ras and Raf), each of them fused either to the receptor or to the chimeric Galpha, allow efficient signal transduction. PMID:9405353

  6. High efficiency furnace

    SciTech Connect

    Hwang, K. S.; Koestler, D. J.

    1985-12-31

    Disclosed is a dwelling furnace having at least one clam-shell type primary heat exchanger in parallel orientation with a secondary heat exchanger, both the primary and secondary heat exchangers being vertically oriented relative to a furnace housing and parallel to the flow of air to be heated. The primary heat exchanger has a combustion chamber in the lower end thereof, and the lower end of the secondary heat exchanger exhausts into a tertiary heat exchanger oriented approximately perpendicular to the primary and secondary heat exchangers and horizontally relative to the housing, below the combustion chambers of the primary heat exchangers and below the exhaust outlet of the secondary heat exchanger. The tertiary heat exchanger includes a plurality of condensation tubes for retrieving the latent heat of condensation of the combustion gases. The furnace further comprises an induced draft blower for drawing combustion gases through the heat exchangers and inducting sufficient air to the combustion chamber of the primary heat exchanger for efficient combustion.

  7. Vitrectomy Before Intravitreal Injection of AAV2/2 Vector Promotes Efficient Transduction of Retinal Ganglion Cells in Dogs and Nonhuman Primates.

    PubMed

    Tshilenge, Kizito-Tshitoko; Ameline, Baptiste; Weber, Michel; Mendes-Madeira, Alexandra; Nedellec, Steven; Biget, Marine; Provost, Nathalie; Libeau, Lyse; Blouin, Véronique; Deschamps, Jack-Yves; Le Meur, Guylène; Colle, Marie-Anne; Moullier, Philippe; Pichard, Virginie; Rolling, Fabienne

    2016-06-01

    Recombinant adeno-associated virus (AAV) has emerged as a promising vector for retinal gene delivery to restore visual function in certain forms of inherited retinal dystrophies. Several studies in rodent models have shown that intravitreal injection of the AAV2/2 vector is the optimal route for efficient retinal ganglion cell (RGC) transduction. However, translation of these findings to larger species, including humans, is complicated by anatomical differences in the eye, a key difference being the comparatively smaller volume of the vitreous chamber in rodents. Here, we address the role of the vitreous body as a potential barrier to AAV2/2 diffusion and transduction in the RGCs of dogs and macaques, two of the most relevant preclinical models. We intravitreally administered the AAV2/2 vector carrying the CMV-eGFP reporter cassette in dog and macaque eyes, either directly into the vitreous chamber or after complete vitrectomy, a surgical procedure that removes the vitreous body. Our findings suggest that the vitreous body appears to trap the injected vector, thus impairing the diffusion and transduction of AAV2/2 to inner retinal neurons. We show that vitrectomy before intravitreal vector injection is an effective means of overcoming this physical barrier, improving the transduction of RGCs in dog and macaque retinas. These findings support the use of vitrectomy in clinical trials of intravitreal gene transfer techniques targeting inner retinal neurons.

  8. High efficiency gas burner

    DOEpatents

    Schuetz, Mark A.

    1983-01-01

    A burner assembly provides for 100% premixing of fuel and air by drawing the air into at least one high velocity stream of fuel without power assist. Specifically, the nozzle assembly for injecting the fuel into a throat comprises a plurality of nozzles in a generally circular array. Preferably, swirl is imparted to the air/fuel mixture by angling the nozzles. The diffuser comprises a conical primary diffuser followed by a cusp diffuser.

  9. Development of automated high throughput single molecular microfluidic detection platform for signal transduction analysis

    NASA Astrophysics Data System (ADS)

    Huang, Po-Jung; Baghbani Kordmahale, Sina; Chou, Chao-Kai; Yamaguchi, Hirohito; Hung, Mien-Chie; Kameoka, Jun

    2016-03-01

    Signal transductions including multiple protein post-translational modifications (PTM), protein-protein interactions (PPI), and protein-nucleic acid interaction (PNI) play critical roles for cell proliferation and differentiation that are directly related to the cancer biology. Traditional methods, like mass spectrometry, immunoprecipitation, fluorescence resonance energy transfer, and fluorescence correlation spectroscopy require a large amount of sample and long processing time. "microchannel for multiple-parameter analysis of proteins in single-complex (mMAPS)"we proposed can reduce the process time and sample volume because this system is composed by microfluidic channels, fluorescence microscopy, and computerized data analysis. In this paper, we will present an automated mMAPS including integrated microfluidic device, automated stage and electrical relay for high-throughput clinical screening. Based on this result, we estimated that this automated detection system will be able to screen approximately 150 patient samples in a 24-hour period, providing a practical application to analyze tissue samples in a clinical setting.

  10. Potential insight for drug discovery from high fidelity receptor-mediated transduction mechanisms in insects

    PubMed Central

    Raffa, Robert B.; Raffa, Kenneth F.

    2011-01-01

    Introduction There is a pervasive and growing concern about the small number of new pharmaceutical agents. There are many proposed explanations for this trend that do not involve the drug-discovery process per se, but the discovery process itself has also come under scrutiny. If the current paradigms are indeed not working, where are novel ideas to come from? Perhaps it is time to look to novel sources. Areas covered The receptor-signaling and 2nd-messenger transduction processes present in insects are quite similar to those in mammals (involving G proteins, ion channels, etc.). However, a review of these systems reveals an unprecedented degree of high potency and receptor selectivity to an extent greater than that modeled in most current drug-discovery approaches. Expert opinion A better understanding of insect receptor pharmacology could stimulate novel theoretical and practical ideas in mammalian pharmacology (drug discovery) and, conversely, the application of pharmacology and medicinal chemistry principles could stimulate novel advances in entomology (safer and more targeted control of pest species). PMID:21984882

  11. C-reactive protein (CRP) is essential for efficient systemic transduction of recombinant adeno-associated virus vector 1 (rAAV-1) and rAAV-6 in mice.

    PubMed

    Denard, Jerome; Marolleau, Beatrice; Jenny, Christine; Rao, Tata Nageswara; Fehling, Hans Jörg; Voit, Thomas; Svinartchouk, Fedor

    2013-10-01

    The clinical relevance of gene therapy using the recombinant adeno-associated virus (rAAV) vectors often requires widespread distribution of the vector, and in this case, systemic delivery is the optimal route of administration. Humoral blood factors, such as antibodies or complement, are the first barriers met by the vectors administered systemically. We have found that other blood proteins, galectin 3 binding protein (G3BP) and C-reactive protein (CRP), can interact with different AAV serotypes in a species-specific manner. While interactions of rAAV vectors with G3BP, antibodies, or complement lead to a decrease in vector efficacy, systemic transduction of the CRP-deficient mouse and its respective control clearly established that binding to mouse CRP (mCRP) boosts rAAV vector 1 (rAAV-1) and rAAV-6 transduction efficiency in skeletal muscles over 10 times. Notably, the high efficacy of rAAV-6 in CRP-deficient mice can be restored by reconstitution of the CRP-deficient mouse with mCRP. Human CRP (hCRP) does not interact with either rAAV-1 or rAAV-6, and, consequently, the high efficiency of mCRP-mediated muscle transduction by these serotypes in mice cannot be translated to humans. No interaction of mCRP or hCRP was observed with rAAV-8 and rAAV-9. We show, for the first time, that serum components can significantly enhance rAAV-mediated tissue transduction in a serotype- and species-specific manner. Bioprocessing in body fluids should be considered when transfer of a preclinical proof of concept for AAV-based gene therapy to humans is planned.

  12. High efficiency centrifugal pump

    SciTech Connect

    Nasvytis, P.J.; Jahrstorfer, G.W.

    1983-10-11

    A high speed fuel pump for a gas turnbine engine has a positively-driven shroud positioned between a main impeller and the wall of a pumping cavity to reduce impeller drag. The shroud is formed by a first disc having a boost impeller connected to its central hub portion and a second disc having a gear carried by its central hub portion. The main drive shaft assembly to which the main impeller is connected, carries a gear which meshes with gear mounted upon a shaft. The shaft also carries a gear which meshes with the gear. The gears are sized so that the shroud is driven at one-half the speed of the main impeller in order to maximize impeller drag reduction and enhance pumping capability when severe inlet conditions are present at the pump inlet.

  13. High efficiency solar cell processing

    NASA Technical Reports Server (NTRS)

    Ho, F.; Iles, P. A.

    1985-01-01

    At the time of writing, cells made by several groups are approaching 19% efficiency. General aspects of the processing required for such cells are discussed. Most processing used for high efficiency cells is derived from space-cell or concentrator cell technology, and recent advances have been obtained from improved techniques rather than from better understanding of the limiting mechanisms. Theory and modeling are fairly well developed, and adequate to guide further asymptotic increases in performance of near conventional cells. There are several competitive cell designs with promise of higher performance ( 20%) but for these designs further improvements are required. The available cell processing technology to fabricate high efficiency cells is examined.

  14. Infliximab reverses steatosis and improves insulin signal transduction in liver of rats fed a high-fat diet.

    PubMed

    Barbuio, Raquel; Milanski, Marciane; Bertolo, Manoel B; Saad, Mário J; Velloso, Lício A

    2007-09-01

    Non-alcoholic fatty liver disease, induced by nutritional factors, is one of the leading causes of hepatic dysfunction in the modern world. The activation of proinflammatory signaling in the liver, which is induced by systemic and locally produced cytokines, and the development of hepatic insulin resistance are two important factors associated with the progression from steatosis to steatohepatitis, a pre-cirrhotic condition. The objective of the present study was to evaluate the effect of inhibition of tumour necrosis factor (TNF)-alpha , using the monoclonal antibody infliximab, on the expression of cytokines, induction of steatosis and fibrosis, and insulin signal transduction in the liver of Wistar rats fed a high-fat diet. Ten days of treatment with infliximab significantly reduced the expression of the proinflammatory markers, TNF-alpha , IL-6, IL-1beta , and SOCS-3, in the liver of rats fed a high-fat diet. This was accompanied by reduced fat deposition and fibrosis and by improved insulin signal transduction through insulin receptor (IR)/IR substrate/Akt/FOXO1 and JAK2/STAT3 pathways. In conclusion, short-term inhibition of TNF-alpha with infliximab reduces inflammation and steatosis/fibrosis, while improving insulin signal transduction in an animal model treated with a high-fat diet.

  15. Advanced high efficiency concentrator cells

    SciTech Connect

    Gale, R. . Varian Research Center)

    1992-06-01

    This report describes research to develop the technology needed to demonstrate a monolithic, multijunction, two-terminal, concentrator solar cell with a terrestrial power conversion efficiency greater than 35%. Under three previous subcontracts, Varian developed many of the aspects of a technology needed to fabricate very high efficiency concentrator cells. The current project was aimed at exploiting the new understanding of high efficiency solar cells. Key results covered in this report are as follows. (1) A 1.93-eV AlGaAs/1.42-eV GaAs metal-interconnected cascade cell was manufactured with a one-sun efficiency at 27.6% at air mass 1.5 (AM1.5) global. (2) A 1.0eV InGaAs cell was fabricated on the reverse'' side of a low-doped GaAs substrate with a one-sun efficiency of 2.5% AM1.5 diffuse and a short-circuit current of 14.4 mA/cm{sup 2}. (3) Small-scale manufacturing of GaAs p/n concentrator cells was attempted and obtained an excellent yield of high-efficiency cells. (4) Grown-in tunnel junction cell interconnects that are transparent and thermally stable using C and Si dopants were developed. 10 refs.

  16. High efficiency SPS klystron design

    NASA Technical Reports Server (NTRS)

    Nalos, E. J.

    1980-01-01

    The most likely compact configuration to realize both high efficiency and high gain (approx. 40 dB) is a 5-6 cavity design focused by an electromagnet. The basic klystron efficiency cannot be expected to exceed 70-75% without collector depression. It was estimated that the net benefit of a 5 stage collector over a 2 stage collector is between 1.5 and 3.5 kW per tube. A modulating anode is incorporated in the design to enable rapid shutoff of the beam current in case the r.f. drive should be removed.

  17. High efficiency solar panel /HESP/

    NASA Technical Reports Server (NTRS)

    Stella, P. M.; Gay, C.; Uno, F.; Scott-Monck, J.

    1978-01-01

    A family of high efficiency, weldable silicon solar cells, incorporating nearly every feature of advanced cell technology developed in the past four years, was produced and subjected to space qualification testing. This matrix contained both field and non-field cells ranging in thickness from 0.10 mm to 0.30 mm, and in base resistivity from nominal two to one hundred ohm-cm. Initial power outputs as high as 20 mW/sq cm (14.8% AM0 efficiency) were produced by certain cell types within the matrix.

  18. High Efficiency Engine Technologies Program

    SciTech Connect

    Rich Kruiswyk

    2010-07-13

    Caterpillar's Product Development and Global Technology Division carried out a research program on waste heat recovery with support from DOE (Department of Energy) and the DOE National Energy Technology Laboratory. The objective of the program was to develop a new air management and exhaust energy recovery system that would demonstrate a minimum 10% improvement in thermal efficiency over a base heavy-duty on-highway diesel truck engine. The base engine for this program was a 2007 C15 15.2L series-turbocharged on-highway truck engine with a LPL (low-pressure loop) exhaust recirculation system. The focus of the program was on the development of high efficiency turbomachinery and a high efficiency turbocompound waste heat recovery system. The focus of each area of development was as follows: (1) For turbine stages, the focus was on investigation and development of technologies that would improve on-engine exhaust energy utilization compared to the conventional radial turbines in widespread use today. (2) For compressor stages, the focus was on investigating compressor wheel design parameters beyond the range typically utilized in production, to determine the potential efficiency benefits thereof. (3) For turbocompound, the focus was on the development of a robust bearing system that would provide higher bearing efficiencies compared to systems used in turbocompound power turbines in production. None of the turbocharger technologies investigated involved addition of moving parts, actuators, or exotic materials, thereby increasing the likelihood of a favorable cost-value tradeoff for each technology. And the turbocompound system requires less hardware addition than competing bottoming cycle technologies, making it a more attractive solution from a cost and packaging standpoint. Main outcomes of the program are as follows: (1) Two turbine technologies that demonstrated up to 6% improvement in turbine efficiency on gas stand and 1-3% improvement in thermal efficiency in

  19. Enabling High Efficiency Ethanol Engines

    SciTech Connect

    Szybist, J.; Confer, K.

    2011-03-01

    Delphi Automotive Systems and ORNL established this CRADA to explore the potential to improve the energy efficiency of spark-ignited engines operating on ethanol-gasoline blends. By taking advantage of the fuel properties of ethanol, such as high compression ratio and high latent heat of vaporization, it is possible to increase efficiency with ethanol blends. Increasing the efficiency with ethanol-containing blends aims to remove a market barrier of reduced fuel economy with E85 fuel blends, which is currently about 30% lower than with petroleum-derived gasoline. The same or higher engine efficiency is achieved with E85, and the reduction in fuel economy is due to the lower energy density of E85. By making ethanol-blends more efficient, the fuel economy gap between gasoline and E85 can be reduced. In the partnership between Delphi and ORNL, each organization brought a unique and complementary set of skills to the project. Delphi has extensive knowledge and experience in powertrain components and subsystems as well as overcoming real-world implementation barriers. ORNL has extensive knowledge and expertise in non-traditional fuels and improving engine system efficiency for the next generation of internal combustion engines. Partnering to combine these knowledge bases was essential towards making progress to reducing the fuel economy gap between gasoline and E85. ORNL and Delphi maintained strong collaboration throughout the project. Meetings were held regularly, usually on a bi-weekly basis, with additional reports, presentations, and meetings as necessary to maintain progress. Delphi provided substantial hardware support to the project by providing components for the single-cylinder engine experiments, engineering support for hardware modifications, guidance for operational strategies on engine research, and hardware support by providing a flexible multi-cylinder engine to be used for optimizing engine efficiency with ethanol-containing fuels.

  20. Efficient transduction of vascular smooth muscle cells with a translational AAV2.5 vector: a new perspective for in-stent restenosis gene therapy.

    PubMed

    Lompré, A-M; Hadri, L; Merlet, E; Keuylian, Z; Mougenot, N; Karakikes, I; Chen, J; Atassi, F; Marchand, A; Blaise, R; Limon, I; McPhee, S W J; Samulski, R J; Hajjar, R J; Lipskaia, L

    2013-09-01

    Coronary artery disease represents the leading cause of mortality in the developed world. Percutaneous coronary intervention involving stent placement remains disadvantaged by restenosis or thrombosis. Vascular gene therapy-based methods may be approached, but lack a vascular gene delivery vector. We report a safe and efficient long-term transduction of rat carotid vessels after balloon injury intervention with a translational optimized AAV2.5 vector. Compared with other known adeno-associated virus (AAV) serotypes, AAV2.5 demonstrated the highest transduction efficiency of human coronary artery vascular smooth muscle cells (VSMCs) in vitro. Local delivery of AAV2.5-driven transgenes in injured carotid arteries resulted in transduction as soon as day 2 after surgery and persisted for at least 30 days. In contrast to adenovirus 5 vector, inflammation was not detected in AAV2.5-transduced vessels. The functional effects of AAV2.5-mediated gene transfer on neointimal thickening were assessed using the sarco/endoplasmic reticulum Ca(2+) ATPase isoform 2a (SERCA2a) human gene, known to inhibit VSMC proliferation. At 30 days, human SERCA2a messenger RNA was detected in transduced arteries. Morphometric analysis revealed a significant decrease in neointimal hyperplasia in AAV2.5-SERCA2a-transduced arteries: 28.36±11.30 (n=8) vs 77.96±24.60 (n=10) μm(2), in AAV2.5-green fluorescent protein-infected, P<0.05. In conclusion, AAV2.5 vector can be considered as a promising safe and effective vector for vascular gene therapy.

  1. Dynamic receptor team formation can explain the high signal transduction gain in Escherichia coli.

    PubMed

    Albert, Réka; Chiu, Yu-Wen; Othmer, Hans G

    2004-05-01

    Evolution has provided many organisms with sophisticated sensory systems that enable them to respond to signals in their environment. The response frequently involves alteration in the pattern of movement, either by directed movement, a process called taxis, or by altering the speed or frequency of turning, which is called kinesis. Chemokinesis has been most thoroughly studied in the peritrichous bacterium Escherichia coli, which has four helical flagella distributed over the cell surface, and swims by rotating them. When rotated counterclockwise the flagella coalesce into a propulsive bundle, producing a relatively straight "run," and when rotated clockwise they fly apart, resulting in a "tumble" which reorients the cell with little translocation. A stochastic process generates the runs and tumbles, and in a chemoeffector gradient, runs that carry the cell in a favorable direction are extended. The cell senses spatial gradients as temporal changes in receptor occupancy and changes the probability of counterclockwise rotation (the bias) on a fast timescale, but adaptation returns the bias to baseline on a slow timescale, enabling the cell to detect and respond to further concentration changes. The overall structure of the signal transduction pathways is well characterized in E. coli, but important details are still not understood. Only recently has a source of gain in the signal transduction network been identified experimentally, and here we present a mathematical model based on dynamic assembly of receptor teams that can explain this observation.

  2. Dynamic Receptor Team Formation Can Explain the High Signal Transduction Gain in Escherichia coli

    PubMed Central

    Albert, Réka; Chiu, Yu-wen; Othmer, Hans G.

    2004-01-01

    Evolution has provided many organisms with sophisticated sensory systems that enable them to respond to signals in their environment. The response frequently involves alteration in the pattern of movement, either by directed movement, a process called taxis, or by altering the speed or frequency of turning, which is called kinesis. Chemokinesis has been most thoroughly studied in the peritrichous bacterium Escherichia coli, which has four helical flagella distributed over the cell surface, and swims by rotating them. When rotated counterclockwise the flagella coalesce into a propulsive bundle, producing a relatively straight “run,” and when rotated clockwise they fly apart, resulting in a “tumble” which reorients the cell with little translocation. A stochastic process generates the runs and tumbles, and in a chemoeffector gradient, runs that carry the cell in a favorable direction are extended. The cell senses spatial gradients as temporal changes in receptor occupancy and changes the probability of counterclockwise rotation (the bias) on a fast timescale, but adaptation returns the bias to baseline on a slow timescale, enabling the cell to detect and respond to further concentration changes. The overall structure of the signal transduction pathways is well characterized in E. coli, but important details are still not understood. Only recently has a source of gain in the signal transduction network been identified experimentally, and here we present a mathematical model based on dynamic assembly of receptor teams that can explain this observation. PMID:15111386

  3. High-power, high-efficiency FELs

    SciTech Connect

    Sessler, A.M.

    1989-04-01

    High power, high efficiency FELs require tapering, as the particles loose energy, so as to maintain resonance between the electromagnetic wave and the particles. They also require focusing of the particles (usually done with curved pole faces) and focusing of the electromagnetic wave (i.e. optical guiding). In addition, one must avoid transverse beam instabilities (primarily resistive wall) and longitudinal instabilities (i.e sidebands). 18 refs., 7 figs., 3 tabs.

  4. Specialized Transduction Designed for Precise High-Throughput Unmarked Deletions in Mycobacterium tuberculosis

    PubMed Central

    Jain, Paras; Hsu, Tsungda; Arai, Masayoshi; Biermann, Karolin; Thaler, David S.; Nguyen, Andrew; González, Pablo A.; Tufariello, Joann M.; Kriakov, Jordan; Chen, Bing; Larsen, Michelle H.

    2014-01-01

    ABSTRACT Specialized transduction has proven to be useful for generating deletion mutants in most mycobacteria, including virulent Mycobacterium tuberculosis. We have improved this system by developing (i) a single-step strategy for the construction of allelic exchange substrates (AES), (ii) a temperature-sensitive shuttle phasmid with a greater cloning capacity than phAE87, and (iii) bacteriophage-mediated transient expression of site-specific recombinase to precisely excise antibiotic markers. The methods ameliorate rate-limiting steps in strain construction in these difficult-to-manipulate bacteria. The new methods for strain construction were demonstrated to generalize to all classes of genes and chromosomal loci by generating more than 100 targeted single- or multiple-deletion substitutions. These improved methods pave the way for the generation of a complete ordered library of M. tuberculosis null strains, where each strain is deleted for a single defined open reading frame in M. tuberculosis. PMID:24895308

  5. High Efficiency Room Air Conditioner

    SciTech Connect

    Bansal, Pradeep

    2015-01-01

    This project was undertaken as a CRADA project between UT-Battelle and Geberal Electric Company and was funded by Department of Energy to design and develop of a high efficiency room air conditioner. A number of novel elements were investigated to improve the energy efficiency of a state-of-the-art WAC with base capacity of 10,000 BTU/h. One of the major modifications was made by downgrading its capacity from 10,000 BTU/hr to 8,000 BTU/hr by replacing the original compressor with a lower capacity (8,000 BTU/hr) but high efficiency compressor having an EER of 9.7 as compared with 9.3 of the original compressor. However, all heat exchangers from the original unit were retained to provide higher EER. The other subsequent major modifications included- (i) the AC fan motor was replaced by a brushless high efficiency ECM motor along with its fan housing, (ii) the capillary tube was replaced with a needle valve to better control the refrigerant flow and refrigerant set points, and (iii) the unit was tested with a drop-in environmentally friendly binary mixture of R32 (90% molar concentration)/R125 (10% molar concentration). The WAC was tested in the environmental chambers at ORNL as per the design rating conditions of AHAM/ASHRAE (Outdoor- 95F and 40%RH, Indoor- 80F, 51.5%RH). All these modifications resulted in enhancing the EER of the WAC by up to 25%.

  6. High Efficiency IMM Solar Cells

    NASA Astrophysics Data System (ADS)

    Sharps, P.; Cho, B.; Chumney, D.; Cornfeild, A.; Guzie, B.; Hazlett, D.; Lin, Y.; Mackos, C.; Patel, P.; Stan, M.; Steinfeldt, J.; Tourino, C.

    2014-08-01

    We review the status of currently available commercial multi-junction cells, review options for next generation high efficiency cell architectures, and present the latest developments on the inverted metamorphic multi- junction (IMM) solar cell. Over 20,000 IMM cells have been prototyped to date, and efficiencies of up to 37% have been measured. We present the most recent performance data, including the response to particle radiation. The IMM cell can be used in a number of rigid or flexible configurations, and considerable effort is currently focused on cell packaging and panel integration. We discuss several design options, including a "drop in" replacement for the current 29.5% ZTJ cell technology. We will also address the reliability and cost of the IMM cell.

  7. High Efficiency Germanium Immersion Gratings

    SciTech Connect

    Kuzmenko, P J; Davis, P J; Little, S L; Little, L M; Bixler, J V

    2006-05-01

    We have fabricated several germanium immersion gratings by single crystal, single point diamond flycutting on an ultra-precision lathe. Use of a dead sharp tool produces groove corners less than 0.1 micron in radius and consequently high diffraction efficiency. We measured first order efficiencies in immersion of over 80% at 10.6 micron wavelength. Wavefront error was low averaging 0.06 wave rms (at 633 nm) across the full aperture. The grating spectral response was free of ghosts down to our detection limit of 1 part in 10{sup 4}. Scatter should be low based upon the surface roughness. Measurement of the spectral line profile of a CO{sub 2} laser sets an upper bound on total integrated scatter of 0.5%.

  8. Lipid and cationic polymer based transduction of botulinum holotoxin, or toxin protease alone, extends the target cell range and improves the efficiency of intoxication.

    PubMed

    Kuo, Chueh-Ling; Oyler, George; Shoemaker, Charles B

    2010-01-01

    Botulinum neurotoxin (BoNT) heavy chain (Hc) facilitates receptor-mediated endocytosis into neuronal cells and transport of the light chain (Lc) protease to the cytosol where neurotransmission is inhibited as a result of SNARE protein cleavage. Here we show that the role of BoNT Hc in cell intoxication can be replaced by commercial lipid-based and polycationic polymer DNA transfection reagents. BoNT "transduction" by these reagents permits efficient intoxication of neuronal cells as well as some non-neuronal cell lines normally refractory to BoNT. Surprisingly, the reagents facilitate delivery of recombinant BoNT Lc protease to the cytosol of both neuronal and non-neuronal cells in the absence of BoNT Hc, and with sensitivities approaching that of BoNT holotoxin. Transduction of BoNT, as with natural intoxication, is inhibited by bafilomycin A1, methylamine and ammonium chloride indicating that both pathways require endosome acidification. DNA transfection reagents facilitate intoxication by holotoxins, or isolated Lc proteases, of all three BoNT serotypes tested (A, B, E). These results suggest that lipid and cationic polymer transfection reagents facilitate cytosolic delivery of BoNT holotoxins and isolated Lc proteases by an endosomal uptake pathway.

  9. Treatment with hydroxyurea and tyrphostin-1 significantly improves the transduction efficiency of recombinant adeno-associated viruses in human cancer cells.

    PubMed

    Kim, Sung Jin; Nam, Young Ran; Shin, Ohkyu; Choi, Jene; Lee, Boyoung; Chang, Jin Woo; Kwon, Yunhee Kim; Park, Keerang; Lee, Heuiran

    2005-12-01

    To enhance the transduction efficiency (TE) of a recombinant adeno-associated virus 2 (rAAV2) in human cancer cells, we examined the combined effects of various chemicals known to influence the rAAV2 transduction process at distinct steps. Among the agents tested were trichostatin A, a histone deacetylase inhibitor, MG-132, a proteosome inhibitor, the genotoxic agents hydroxyurea, aphidicolin, etoposide and camptothecin, and tyrphostin-1, an epidermal growth factor receptor inhibitor. During or after chemical treatment, various human cancer cells were infected with rAAV2 expressing beta-galactosidase. Treatment with hydroxy-urea or etoposide plus tyrphostin-1 dramatically increased the TE in most cell lines. The combination of hydroxyurea plus tyrphostin-1 increased TE to 37.7+/-7.9%, 32.8+/-2.0% and 31.8+/-2.1% in SK-Hep1, HeLa, and HCT116 cells, respectively. In addition, following rAAV2 infection and treatment with hydroxyurea plus tyrphostin-1, long-term transgene expression was observed for up to 6 months, with no damage to the transduced cells. These results indicate that rAAV2 transgene expression can be significantly enhanced by a combination of chemical agents with distinct activity and prolonged gene expression can occur following rAAV2 gene transfer into human cancer cells.

  10. A simple, high efficiency, high resolution spectropolarimeter

    NASA Astrophysics Data System (ADS)

    Barden, Samuel C.

    2012-09-01

    A simple concept is described that uses volume phase holographic gratings as polarizing dispersers for a high efficiency, high resolution spectropolarimeter. Although the idea has previously been mentioned in the literature as possible, such a concept has not been explored in detail. Performance analysis is presented for a VPHG spectropolarimeter concept that could be utilized for both solar and night-time astronomy. Instrumental peak efficiency can approach 100% with spectral dispersions permitting R~200,000 spectral resolution with diffraction limited telescopes. The instrument has 3-channels: two dispersed image planes with orthogonal polarization and an undispersed image plane. The concept has a range of versatility where it could be configured (with appropriate half-wave plates) for slit-fed spectroscopy or without slits for snapshot/hyperspectral/tomographic spectroscopic imaging. Multiplex gratings could also be used for the simultaneous recording of two separate spectral bands or multiple instruments could be daisy chained with beam splitters for further spectral coverage.

  11. High efficiency motor rewind study

    NASA Astrophysics Data System (ADS)

    Wallace, A. K.; Spee, R.

    1991-02-01

    The objective of performing this work was to evaluate a new technology used for rewinding electric motors. Motor performance evaluation was conducted at the motor test facility at Oregon State University. The test program consisted of comparing new high efficiency motor technology and standard rewind technology with the Unity-Plus system. The Unity-Plus configuration exhibited reduced efficiency over the complete load range compared to the other motors. Appropriately sized capacitors connected to the terminals of the conventional induction motor produced the same power factor improvement as the Unity-Plus system. Torque production and torque pulsation were very similar for all systems. The Unity-Plus configuration drew lower starting currents but the duration of the starting transient was increased. Motor temperature rise was about the same for all systems. Noise levels were about the same in all systems. Although determination of time to failure was not undertaken, the expected lifetime of the Unit-Plus system is probably less due to higher capacitor stress and higher insulation stress. The investigation concludes that a conventional induction motor with terminal capacitors is the most acceptable way of obtaining good efficiency and power factor and the Unity-Plus system cannot be recommended on the basis of any of the evaluation criteria used in this study.

  12. High-efficiency photoionization detector

    SciTech Connect

    Anderson, D.F.

    1981-05-12

    A high efficiency photoionization detector using tetraaminoethylenes in a gaseous state having a low ionization potential and a relative photoionization cross section which closely matches the emission spectrum of xenon gas. Imaging proportional counters are also disclosed using the novel photoionization detector of the invention. The compound of greatest interest is TMAE which comprises tetrakis(dimethylamino)ethylene which has a measured ionization potential of 5.36 +- 0.02 eV, and a vapor pressure of 0.35 torr at 20/sup 0/C.

  13. High-efficiency photovoltaic cells

    DOEpatents

    Yang, H.T.; Zehr, S.W.

    1982-06-21

    High efficiency solar converters comprised of a two cell, non-lattice matched, monolithic stacked semiconductor configuration using optimum pairs of cells having bandgaps in the range 1.6 to 1.7 eV and 0.95 to 1.1 eV, and a method of fabrication thereof, are disclosed. The high band gap subcells are fabricated using metal organic chemical vapor deposition (MOCVD), liquid phase epitaxy (LPE) or molecular beam epitaxy (MBE) to produce the required AlGaAs layers of optimized composition, thickness and doping to produce high performance, heteroface homojunction devices. The low bandgap subcells are similarly fabricated from AlGa(As)Sb compositions by LPE, MBE or MOCVD. These subcells are then coupled to form a monolithic structure by an appropriate bonding technique which also forms the required transparent intercell ohmic contact (IOC) between the two subcells. Improved ohmic contacts to the high bandgap semiconductor structure can be formed by vacuum evaporating to suitable metal or semiconductor materials which react during laser annealing to form a low bandgap semiconductor which provides a low contact resistance structure.

  14. High-efficiency dielectrophoretic ratchet.

    PubMed

    Germs, Wijnand Chr; Roeling, Erik M; van Ijzendoorn, Leo J; Smalbrugge, Barry; de Vries, Tjibbe; Geluk, Erik Jan; Janssen, René A J; Kemerink, Martijn

    2012-10-01

    Brownian ratchets enable the use of thermal motion in performing useful work. They typically employ spatial asymmetry to rectify nondirected external forces that drive the system out of equilibrium (cf. running marbles on a shaking washboard). The major application foreseen for Brownian ratchets is high-selectivity fractionation of particle or molecule distributions. Here, we investigate the functioning of an important model system, the on/off ratchet for water-suspended particles, in which interdigitated finger electrodes can be switched on and off to create a time-dependent, spatially periodic but asymmetric potential. Surprisingly, we find that mainly dielectrophoretic rather than electrophoretic forces are responsible for the ratchet effect. This has major implications for the (a)symmetry of the ratchet potential and the settings needed for optimal performance. We demonstrate that by applying a potential offset the ratchet can be optimized such that its particle displacement efficiency reaches the theoretical upper limit corresponding to the electrode geometry and particle size. Efficient fractionation based on size selectivity is therefore not only possible for charged species, but also for uncharged ones, which greatly expands the applicability range of this type of Brownian ratchet.

  15. High efficiency shale oil recovery

    SciTech Connect

    Adams, C.D.

    1992-07-18

    The overall project objective is to demonstrate the high efficiency of the Adams Counter-Current shale oil recovery process. The efficiency will first be demonstrated at bench-scale, in the current phase, after which the demonstration will be extended to the operation of a small pilot plant. Thus the immediate project objective is to obtain data on oil shale retorting operations in a small batch rotary kiln that will be representative of operations in the proposed continuous process pilot plant. Although an oil shale batch sample is sealed in the batch kiln from the start until the end of the run, the process conditions for the batch are the same as the conditions that an element of oil shale would encounter in a larger continuous process kiln. For example, similar conditions of heatup rate, oxidation of the residue and cool-down prevail for the element in both systems. This batch kiln is a unit constructed in a 1987 Phase I SBIR tar sand retorting project. The kiln worked fairly well in that project; however, the need for certain modifications was observed. These modifications are now underway to simplify the operation and make the data and analysis more exact. The second quarter agenda consisted of (a) kiln modifications; (b) sample preparation; and (c) Heat Transfer calibration runs (part of proposal task number 3 -- to be completed by the end of month 7).

  16. High efficiency laser spectrum conditioner

    DOEpatents

    Greiner, Norman R.

    1980-01-01

    A high efficiency laser spectrum conditioner for generating a collinear parallel output beam containing a predetermined set of frequencies from a multifrequency laser. A diffraction grating and spherical mirror are used in combination, to disperse the various frequencies of the input laser beam and direct these frequencies along various parallel lines spatially separated from one another to an apertured mask. Selection of the desired frequencies is accomplished by placement of apertures at locations on the mask where the desired frequencies intersect the mask. A recollimated parallel output beam with the desired set of frequencies is subsequently generated utilizing a mirror and grating matched and geometrically aligned in the same manner as the input grating and mirror.

  17. High efficiency ozone generation system

    SciTech Connect

    Karlson, E.L.

    1990-01-09

    This final report entails research prepared to verify the workings and the efficiency of producing ozone with the ELK'' Ozone Generator, which operates at an elevated gas pressure of up to 20 MPA (3000 psi) and is an improvement of the corona discharge ozone generator. The increased pressure produces an increase in the density of oxygen gas fed into the generator. This, in turn, leads to an increased yield of ozone in the ozone oxygen gas mixture leaving the generator. The design of this new ozone generator incorporates a novel positioning of the dielectric to preserve its mechanical integrity at high operating pressures and also incorporates a novel heat removal technique. A large number of ozone production runs have been made at different pressures. Large populations of data such as, temperature points throughout the generator, gas flow, cooling water flow parameters, operating gas pressure, ozone concentration, and data on the dielectric cooling, have been compiled and fed into our computer. This new data indicates not only that high pressures used in a controlled fashion will produce more ozone per watt hour but also indicates what problems exist when pressures are increased, such as the generation of high temperatures not only in the area of ozone generation but within the dielectric. The data also shows the necessary residence time for maximum ozone production at a particular pressure, voltage, temperature and electrode spacing. 14 refs., 22 figs.

  18. In Vivo Stable Transduction of Humanized Liver Tissue in Chimeric Mice via High-Capacity Adenovirus–Lentivirus Hybrid Vector

    PubMed Central

    Kataoka, Miho; Tateno, Chise; Yoshizato, Katsutoshi; Kawasaki, Yoshiko; Kimura, Takahiro; Faure-Kumar, Emmanuelle; Palmer, Donna J.; Ng, Philip; Okamura, Haruki; Kasahara, Noriyuki

    2010-01-01

    Abstract We developed hybrid vectors employing high-capacity adenovirus as a first-stage carrier encoding all the components required for in situ production of a second-stage lentivirus, thereby achieving stable transgene expression in secondary target cells. Such vectors have never previously been tested in normal tissues, because of the scarcity of suitable in vivo systems permissive for second-stage lentivirus assembly. Here we employed a novel murine model in which endogenous liver tissue is extensively reconstituted with engrafted human hepatocytes, and successfully achieved stable transduction by the second-stage lentivirus produced in situ from first-stage adenovirus. This represents the first demonstration of the functionality of adenoviral-lentiviral hybrid vectors in a normal parenchymal organ in vivo. PMID:19725756

  19. A method to obtain the thermal parameters and the photothermal transduction efficiency in an optical hyperthermia device based on laser irradiation of gold nanoparticles.

    PubMed

    Sánchez López de Pablo, Cristina; Olmedo, José Javier Serrano; Rosales, Alejandra Mina; Ramírez Hernández, Norma; Del Pozo Guerrero, Francisco

    2014-01-01

    Optical hyperthermia systems based on the laser irradiation of gold nanorods seem to be a promising tool in the development of therapies against cancer. After a proof of concept in which the authors demonstrated the efficiency of this kind of systems, a modeling process based on an equivalent thermal-electric circuit has been carried out to determine the thermal parameters of the system and an energy balance obtained from the time-dependent heating and cooling temperature curves of the irradiated samples in order to obtain the photothermal transduction efficiency. By knowing this parameter, it is possible to increase the effectiveness of the treatments, thanks to the possibility of predicting the response of the device depending on the working configuration. As an example, the thermal behavior of two different kinds of nanoparticles is compared. The results show that, under identical conditions, the use of PEGylated gold nanorods allows for a more efficient heating compared with bare nanorods, and therefore, it results in a more effective therapy.

  20. A method to obtain the thermal parameters and the photothermal transduction efficiency in an optical hyperthermia device based on laser irradiation of gold nanoparticles

    NASA Astrophysics Data System (ADS)

    Sánchez López de Pablo, Cristina; Serrano Olmedo, José Javier; Mina Rosales, Alejandra; Ramírez Hernández, Norma; del Pozo Guerrero, Francisco

    2014-08-01

    Optical hyperthermia systems based on the laser irradiation of gold nanorods seem to be a promising tool in the development of therapies against cancer. After a proof of concept in which the authors demonstrated the efficiency of this kind of systems, a modeling process based on an equivalent thermal-electric circuit has been carried out to determine the thermal parameters of the system and an energy balance obtained from the time-dependent heating and cooling temperature curves of the irradiated samples in order to obtain the photothermal transduction efficiency. By knowing this parameter, it is possible to increase the effectiveness of the treatments, thanks to the possibility of predicting the response of the device depending on the working configuration. As an example, the thermal behavior of two different kinds of nanoparticles is compared. The results show that, under identical conditions, the use of PEGylated gold nanorods allows for a more efficient heating compared with bare nanorods, and therefore, it results in a more effective therapy.

  1. A method to obtain the thermal parameters and the photothermal transduction efficiency in an optical hyperthermia device based on laser irradiation of gold nanoparticles

    PubMed Central

    2014-01-01

    Optical hyperthermia systems based on the laser irradiation of gold nanorods seem to be a promising tool in the development of therapies against cancer. After a proof of concept in which the authors demonstrated the efficiency of this kind of systems, a modeling process based on an equivalent thermal-electric circuit has been carried out to determine the thermal parameters of the system and an energy balance obtained from the time-dependent heating and cooling temperature curves of the irradiated samples in order to obtain the photothermal transduction efficiency. By knowing this parameter, it is possible to increase the effectiveness of the treatments, thanks to the possibility of predicting the response of the device depending on the working configuration. As an example, the thermal behavior of two different kinds of nanoparticles is compared. The results show that, under identical conditions, the use of PEGylated gold nanorods allows for a more efficient heating compared with bare nanorods, and therefore, it results in a more effective therapy. PMID:25232297

  2. High efficiency shale oil recovery

    SciTech Connect

    Adams, D.C.

    1993-04-22

    The overall project objective is to demonstrate the high efficiency of the Adams Counter-Current shale oil recovery process. The efficiency will first be demonstrated on a small scale, in the current phase, after which the demonstration will be extended to the operation of a small pilot plant. Thus the immediate project objective is to obtain data on oil shale retorting operations in a small batch rotary kiln that will be representative of operations in the proposed continuous process pilot plant. Although an oil shale batch sample is sealed in the batch kiln from the start until the end of the run, the process conditions for the batch are the same as the conditions that an element of oil shale would encounter in a continuous process kiln. Similar chemical and physical conditions (heating, mixing, pyrolysis, oxidation) exist in both systems.The two most important data objectives in this phase of the project are to demonstrate (1) that the heat recovery projected for this project is reasonable and (2) that an oil shale kiln will run well and not plug up due to sticking and agglomeration. The following was completed this quarter. (1) Twelve pyrolysis runs were made on five different oil shales. All of the runs exhibited a complete absence of any plugging, tendency. Heat transfer for Green River oil shale in the rotary kiln was 84.6 Btu/hr/ft[sup 2]/[degrees]F, and this will provide for ample heat exchange in the Adams kiln. (2) One retorted residue sample was oxidized at 1000[degrees]F. Preliminary indications are that the ash of this run appears to have been completely oxidized. (3) Further minor equipment repairs and improvements were required during the course of the several runs.

  3. High efficiency shale oil recovery

    SciTech Connect

    Adams, D.C.

    1992-01-01

    The overall project objective is to demonstrate the high efficiency of the Adams Counter-Current shale oil recovery process. The efficiency will first be demonstrated at bench-scale, in the current phase, after which the demonstration will be extended to the operation of a small pilot plant. Thus the immediate project objective is to obtain data on oil shale retorting operations in a small batch rotary kiln that will be representative of operations in the proposed continuous process pilot plant. Although a batch oil shale sample will be sealed in the batch kiln from the start until the end of the run, the process conditions for the batch will be the same as the conditions that an element of oil shale would encounter in a large continuous process kiln. For example, similar conditions of heat-up rate (20 deg F/min during the pyrolysis), oxidation of the residue and cool-down will prevail for the element in both systems. This batch kiln is a unit constructed in a 1987 Phase I SBIR tar sand retorting project. The kiln worked fairly well in that project; however, the need for certain modifications was observed. These modifications are now underway to simplify the operation and make the data and analysis more exact. The agenda for the first three months of the project consisted of the first of nine tasks and was specified as the following four items: 1. Sample acquisition and equipment alteration: Obtain seven oil shale samples, of varying grade each 10 lb or more, and samples of quartz sand. Order equipment for kiln modification. 3. Set up and modify kiln for operation, including electric heaters on the ends of the kiln. 4. Connect data logger and make other repairs and changes in rotary batch kiln.

  4. High efficiency shale oil recovery

    SciTech Connect

    Adams, D.C.

    1992-01-01

    The overall project objective is to demonstrate the high efficiency of the Adams Counter-Current shale oil recovery process. The efficiency will first be demonstrated on a small scale, in the current phase, after which the demonstration will be extended to the operation of a small pilot plant. Thus the immediate project objective is to obtain data on oil shale retorting operations in a small batch rotary kiln that will be representative of operations in the proposed continuous process pilot plant. Although an oil shale batch sample is sealed in the batch kiln from the start until the end of the run, the process conditions for the batch are the same as the conditions that an element of oil shale would encounter in a continuous process kiln. Similar chemical and physical (heating, mixing) conditions exist in both systems. The two most important data objectives in this phase of the project are to demonstrate (1) that the heat recovery projected for this project is reasonable and (2) that an oil shale kiln will run well and not plug up due to sticking and agglomeration. The following was completed and is reported on this quarter: (1) A software routine was written to eliminate intermittently inaccurate temperature readings. (2) We completed the quartz sand calibration runs, resolving calibration questions from the 3rd quarter. (3) We also made low temperature retorting runs to identify the need for certain kiln modifications and kiln modifications were completed. (4) Heat Conductance data on two Pyrolysis runs were completed on two samples of Occidental oil shale.

  5. High Efficiency Cascade Solar Cells

    SciTech Connect

    Shuguang Deng, Seamus Curran, Igor Vasiliev

    2010-09-28

    This report summarizes the main work performed by New Mexico State University and University of Houston on a DOE sponsored project High Efficiency Cascade Solar Cells. The main tasks of this project include materials synthesis, characterization, theoretical calculations, organic solar cell device fabrication and test. The objective of this project is to develop organic nano-electronic-based photovoltaics. Carbon nanotubes and organic conjugated polymers were used to synthesize nanocomposites as the new active semiconductor materials that were used for fabricating two device architectures: thin film coating and cascade solar cell fiber. Chemical vapor deposition technique was employed to synthesized a variety of carbon nanotubes (single-walled CNT, doubled-walled CNT, multi-walled CNT, N-doped SWCNT, DWCNT and MWCNT, and B-doped SWCNT, DWCNT and MWCNT) and a few novel carbon structures (CNT-based nanolance, nanocross and supported graphene film) that have potential applications in organic solar cells. Purification procedures were developed for removing amorphous carbons from carbon nanotubes, and a controlled oxidation method was established for partial truncation of fullerene molecules. Carbon nanotubes (DWCNT and DWCNT) were functionalized with fullerenes and dyes covalently and used to form nanocomposites with conjugated polymers. Biologically synthesized Tellurium nanotubes were used to form composite with the conjugated polymers as well, which generated the highest reported optical limiting values from composites. Several materials characterization technique including SEM/TEM, Raman, AFM, UV-vis, adsorption and EDS were employed to characterize the physical and chemical properties of the carbon nanotubes, the functionalized carbon nanotubes and the nanocomposites synthesized in this project. These techniques allowed us to have a spectroscopic and morphological control of the composite formation and to understand the materials assembled. A parallel 136-CPU

  6. In vivo protein transduction: delivery of PEP-1-SOD1 fusion protein into myocardium efficiently protects against ischemic insult.

    PubMed

    Zhang, You-En; Wang, Jia-Ning; Tang, Jun-Ming; Guo, Ling-Yun; Yang, Jian-Ye; Huang, Yong-Zhang; Tan, Yan; Fu, Shou-Zhi; Kong, Xia; Zheng, Fei

    2009-02-28

    Myocardial ischemia-reperfusion injury is a medical problem occurring as damage to the myocardium following blood flow restoration after a critical period of coronary occlusion. Oxygen free radicals (OFR) are implicated in reperfusion injury after myocardial ischemia. The antioxidant enzyme, Cu, Zn-superoxide dismutase (Cu, Zn-SOD, also called SOD1) is one of the major means by which cells counteract the deleterious effects of OFR after ischemia. Recently, we reported that a PEP-1-SOD1 fusion protein was efficiently delivered into cultured cells and isolated rat hearts with ischemia-reperfusion injury. In the present study, we investigated the protective effects of the PEP-1-SOD1 fusion protein after ischemic insult. Immunofluorescecnce analysis revealed that the expressed and purified PEP-1-SOD1 fusion protein injected into rat tail veins was efficiently transduced into the myocardium with its native protein structure intact. When injected into Sprague-Dawley rat tail veins, the PEP-1- SOD1 fusion protein significantly attenuated myocardial ischemia-reperfusion damage; characterized by improving cardiac function of the left ventricle, decreasing infarct size, reducing the level of malondialdehyde (MDA), decreasing the release of creatine kinase (CK) and lactate dehydrogenase (LDH), and relieving cardiomyocyte apoptosis. These results suggest that the biologically active intact forms of PEP-1-SOD1 fusion protein will provide an efficient strategy for therapeutic delivery in various diseases related to SOD1 or to OFR.

  7. Towards highly efficient water photoelectrolysis

    NASA Astrophysics Data System (ADS)

    Elavambedu Prakasam, Haripriya

    ethylene glycol resulted in remarkable growth characteristics of titania nanotube arrays, hexagonal closed packed up to 1 mm in length, with tube aspect ratios of approximately 10,000. For the first time, complete anodization of the starting titanium foil has been demonstrated resulting in back to back nanotube array membranes ranging from 360 mum--1 mm in length. The nanotubes exhibited growth rates of up to 15 mum/hr. A detailed study on the factors affecting the growth rate and nanotube dimensions is presented. It is suggested that faster high field ionic conduction through a thinner barrier layer is responsible for the higher growth rates observed in electrolytes containing ethylene glycol. Methods to fabricate free standing, titania nanotube array membranes ranging in thickness from 50 microm--1000 mum has also been an outcome of this dissertation. In an effort to combine the charge transport properties of titania with the light absorption properties of iron (III) oxide, films comprised of vertically oriented Ti-Fe-O nanotube arrays on FTO coated glass substrates have been successfully synthesized in ethylene glycol electrolytes. Depending upon the Fe content the bandgap of the resulting films varied from about 3.26 to 2.17 eV. The Ti-Fe oxide nanotube array films demonstrated a photocurrent of 2 mA/cm2 under global AM 1.5 illumination with a 1.2% (two-electrode) photoconversion efficiency, demonstrating a sustained, time-energy normalized hydrogen evolution rate by water splitting of 7.1 mL/W·hr in a 1 M KOH solution with a platinum counter electrode under an applied bias of 0.7 V. The Ti-Fe-O material architecture demonstrates properties useful for hydrogen generation by water photoelectrolysis and, more importantly, this dissertation demonstrates that the general nanotube-array synthesis technique can be extended to other ternary oxide compositions of interest for water photoelectrolysis.

  8. Efficiency and reliability assessments of retrofitted high-efficiency motors

    SciTech Connect

    Hsu, John S.; Otaduy, P.J.; Dueck, J.D.

    1994-12-31

    The majority of electric-motor applications are pumps, fans, blowers, and certain compressors that follow the load torque pattern described in this paper. It has been known for many years that simply replacing the old motor with a high-efficiency motor might not produce the expected efficiency gain. This paper suggests the calculations for the effective efficiency and temperature rise of the high-efficiency motor. The reliability in terms of temperature rise, downsizing, power factor, harmonics, mechanical structure, etc., are discussed.

  9. High efficiency, long life terrestrial solar panel

    NASA Technical Reports Server (NTRS)

    Chao, T.; Khemthong, S.; Ling, R.; Olah, S.

    1977-01-01

    The design of a high efficiency, long life terrestrial module was completed. It utilized 256 rectangular, high efficiency solar cells to achieve high packing density and electrical output. Tooling for the fabrication of solar cells was in house and evaluation of the cell performance was begun. Based on the power output analysis, the goal of a 13% efficiency module was achievable.

  10. High-efficiency wind turbine

    NASA Technical Reports Server (NTRS)

    Hein, L. A.; Myers, W. N.

    1980-01-01

    Vertical axis wind turbine incorporates several unique features to extract more energy from wind increasing efficiency 20% over conventional propeller driven units. System also features devices that utilize solar energy or chimney effluents during periods of no wind.

  11. High efficiency turbine blade coatings

    SciTech Connect

    Youchison, Dennis L.; Gallis, Michail A.

    2014-06-01

    The development of advanced thermal barrier coatings (TBCs) of yttria stabilized zirconia (YSZ) that exhibit lower thermal conductivity through better control of electron beam - physical vapor deposition (EB-PVD) processing is of prime interest to both the aerospace and power industries. This report summarizes the work performed under a two-year Lab-Directed Research and Development (LDRD) project (38664) to produce lower thermal conductivity, graded-layer thermal barrier coatings for turbine blades in an effort to increase the efficiency of high temperature gas turbines. This project was sponsored by the Nuclear Fuel Cycle Investment Area. Therefore, particular importance was given to the processing of the large blades required for industrial gas turbines proposed for use in the Brayton cycle of nuclear plants powered by high temperature gas-cooled reactors (HTGRs). During this modest (~1 full-time equivalent (FTE)) project, the processing technology was developed to create graded TBCs by coupling ion beam-assisted deposition (IBAD) with substrate pivoting in the alumina-YSZ system. The Electron Beam - 1200 kW (EB-1200) PVD system was used to deposit a variety of TBC coatings with micron layered microstructures and reduced thermal conductivity below 1.5 W/m.K. The use of IBAD produced fully stoichiometric coatings at a reduced substrate temperature of 600°C and a reduced oxygen background pressure of 0.1 Pa. IBAD was also used to successfully demonstrate the transitioning of amorphous PVD-deposited alumina to the -phase alumina required as an oxygen diffusion barrier and for good adhesion to the substrate Ni2Al3 bondcoat. This process replaces the time consuming thermally grown oxide formation required before the YSZ deposition. In addition to the process technology, Direct Simulation Monte Carlo plume modeling and spectroscopic characterization of the PVD plumes were performed. The project consisted of five tasks. These included the

  12. Towards high efficiency heliostat fields

    NASA Astrophysics Data System (ADS)

    Arbes, Florian; Wöhrbach, Markus; Gebreiter, Daniel; Weinrebe, Gerhard

    2017-06-01

    CSP power plants have great potential to substantially contribute to world energy supply. To set this free, cost reductions are required for future projects. Heliostat field layout optimization offers a great opportunity to improve field efficiency. Field efficiency primarily depends on the positions of the heliostats around the tower, commonly known as the heliostat field layout. Heliostat shape also influences efficiency. Improvements to optical efficiency results in electricity cost reduction without adding any extra technical complexity. Due to computational challenges heliostat fields are often arranged in patterns. The mathematical models of the radial staggered or spiral patterns are based on two parameters and thus lead to uniform patterns. Optical efficiencies of a heliostat field do not change uniformly with the distance to the tower, they even differ in the northern and southern field. A fixed pattern is not optimal in many parts of the heliostat field, especially when used as large scaled heliostat field. In this paper, two methods are described which allow to modify field density suitable to inconsistent field efficiencies. A new software for large scale heliostat field evaluation is presented, it allows for fast optimizations of several parameters for pattern modification routines. It was used to design a heliostat field with 23,000 heliostats, which is currently planned for a site in South Africa.

  13. α2,3 and α2,6 N-Linked Sialic Acids Facilitate Efficient Binding and Transduction by Adeno-Associated Virus Types 1 and 6

    PubMed Central

    Wu, Zhijian; Miller, Edward; Agbandje-McKenna, Mavis; Samulski, Richard Jude

    2006-01-01

    Recombinant adeno-associated viruses (AAVs) are promising vectors in the field of gene therapy. Different AAV serotypes display distinct tissue tropism, believed to be related to the distribution of their receptors on target cells. Of the 11 well-characterized AAV serotypes, heparan sulfate proteoglycan and sialic acid have been suggested to be the attachment receptors for AAV type 2 and types 4 and 5, respectively. In this report, we identify the receptor for the two closely related serotypes, AAV1 and AAV6. First, we demonstrate using coinfection experiments and luciferase reporter analysis that AAV1 and AAV6 compete for similar receptors. Unlike heparin sulfate, enzymatic or genetic removal of sialic acid markedly reduced AAV1 and AAV6 binding and transduction. Further analysis using lectin staining and lectin competition assays identified that AAV1 and AAV6 use either α2,3-linked or α2,6-linked sialic acid when transducing numerous cell types (HepG2, Pro-5, and Cos-7). Treatment of cells with proteinase K but not glycolipid inhibitor reduced AAV1 and AAV6 infection, supporting the hypothesis that the sialic acid that facilitates infection is associated with glycoproteins rather than glycolipids. In addition, we determined by inhibitor (N-benzyl GalNAc)- and cell line-specific (Lec-1) studies that AAV1 and AAV6 require N-linked and not O-linked sialic acid. Furthermore, a resialylation experiment on a deficient Lec-2 cell line confirmed a 2,3 and 2,6 N-linked sialic acid requirement, while studies of mucin with O-linked sialic acid showed no inhibition effect for AAV1 and AAV6 transduction on Cos-7 cells. Finally, using a glycan array binding assay we determined that AAV1 efficiently binds to NeuAcα2-3GalNAcβ1-4GlcNAc, as well as two glycoproteins with α2,3 and α2,6 N-linked sialic acids. Taken together, competition, genetic, inhibitor, enzymatic reconstitution, and glycan array experiments support α2,3 and α2,6 sialic acids that are present on N

  14. High Energy Efficiency Air Conditioning

    SciTech Connect

    Edward McCullough; Patrick Dhooge; Jonathan Nimitz

    2003-12-31

    This project determined the performance of a new high efficiency refrigerant, Ikon B, in a residential air conditioner designed to use R-22. The refrigerant R-22, used in residential and small commercial air conditioners, is being phased out of production in developed countries beginning this year because of concerns regarding its ozone depletion potential. Although a replacement refrigerant, R-410A, is available, it operates at much higher pressure than R-22 and requires new equipment. R-22 air conditioners will continue to be in use for many years to come. Air conditioning is a large part of expensive summer peak power use in many parts of the U.S. Previous testing and computer simulations of Ikon B indicated that it would have 20 - 25% higher coefficient of performance (COP, the amount of cooling obtained per energy used) than R-22 in an air-cooled air conditioner. In this project, a typical new R-22 residential air conditioner was obtained, installed in a large environmental chamber, instrumented, and run both with its original charge of R-22 and then with Ikon B. In the environmental chamber, controlled temperature and humidity could be maintained to obtain repeatable and comparable energy use results. Tests with Ikon B included runs with and without a power controller, and an extended run for several months with subsequent analyses to check compatibility of Ikon B with the air conditioner materials and lubricant. Baseline energy use of the air conditioner with its original R-22 charge was measured at 90 deg F and 100 deg F. After changeover to Ikon B and a larger expansion orifice, energy use was measured at 90 deg F and 100 deg F. Ikon B proved to have about 19% higher COP at 90 deg F and about 26% higher COP at 100 deg F versus R-22. Ikon B had about 20% lower cooling capacity at 90 deg F and about 17% lower cooling capacity at 100 deg F versus R-22 in this system. All results over multiple runs were within 1% relative standard deviation (RSD). All of these

  15. Single-crystal lead magnesium niobate-lead titanate (PMN/PT) as a broadband high power transduction material.

    PubMed

    Moffett, Mark B; Robinson, Harold C; Powers, James M; Baird, P David

    2007-05-01

    Two experimental underwater acoustic projectors, a tonpilz array, and a cylindrical line array, were built with single crystal, lead magnesium niobate/lead titanate, a piezoelectric transduction material possessing a large electromechanical coupling factor (k33 = 0.9). The mechanical quality factor, Q(m), and the effective coupling factor, k(eff), determine the frequency band over which high power can be transmitted; k(eff) cannot be greater than the piezoelectric material value, and so a high material coupling factor is a requisite for broadband operation. Stansfield's bandwidth criteria are used to calculate the optimum Q(m) value, Q(opt) approximately 1.2 (1-k(eff)2 1/2/k(eff). The results for the tonpilz projector exhibited k(eff) = 0.730, Q(m) = 1.17 (very near optimal), and a fractional bandwidth of 0.93. For the cylindrical transducer array, k(eff) = 0.867, Q(m) = 0.91 (larger than the optimum value, 0.7), and the bandwidth was 1.16. Although the measured bandwidths were less than optimal, they were accurately predicted by the theory, despite the highly simplified nature of the Van Dyke equivalent circuit, on which the theory is based.

  16. High efficiency stationary hydrogen storage

    SciTech Connect

    Hynek, S.; Fuller, W.; Truslow, S.

    1995-09-01

    Stationary storage of hydrogen permits one to make hydrogen now and use it later. With stationary hydrogen storage, one can use excess electrical generation capacity to power an electrolyzer, and store the resultant hydrogen for later use or transshipment. One can also use stationary hydrogen as a buffer at fueling stations to accommodate non-steady fueling demand, thus permitting the hydrogen supply system (e.g., methane reformer or electrolyzer) to be sized to meet the average, rather than the peak, demand. We at ADL designed, built, and tested a stationary hydrogen storage device that thermally couples a high-temperature metal hydride to a phase change material (PCM). The PCM captures and stores the heat of the hydriding reaction as its own heat of fusion (that is, it melts), and subsequently returns that heat of fusion (by freezing) to facilitate the dehydriding reaction. A key component of this stationary hydrogen storage device is the metal hydride itself. We used nickel-coated magnesium powder (NCMP) - magnesium particles coated with a thin layer of nickel by means of chemical vapor deposition (CVD). Magnesium hydride can store a higher weight fraction of hydrogen than any other practical metal hydride, and it is less expensive than any other metal hydride. We designed and constructed an experimental NCM/PCM reactor out of 310 stainless steel in the form of a shell-and-tube heat exchanger, with the tube side packed with NCMP and the shell side filled with a eutectic mixture of NaCL, KCl, and MgCl{sub 2}. Our experimental results indicate that with proper attention to limiting thermal losses, our overall efficiency will exceed 90% (DOE goal: >75%) and our overall system cost will be only 33% (DOE goal: <50%) of the value of the delivered hydrogen. It appears that NCMP can be used to purify hydrogen streams and store hydrogen at the same time. These prospects make the NCMP/PCM reactor an attractive component in a reformer-based hydrogen fueling station.

  17. Meeting Report: Teaching Signal Transduction

    PubMed Central

    Kramer, IJsbrand; Thomas, Geraint

    2006-01-01

    In July, 2005, the European Institute of Chemistry and Biology at the campus of the University of Bordeaux, France, hosted a focused week of seminars, workshops, and discussions around the theme of “teaching signal transduction.” The purpose of the summer school was to offer both junior and senior university instructors a chance to reflect on the development and delivery of their teaching activities in this area. This was achieved by combining open seminars with restricted access workshops and discussion events. The results suggest ways in which systems biology, information and communication technology, Web-based investigations, and high standard illustrations might be more effectively and efficiently incorporated into modern cell biology courses. PMID:17012185

  18. High efficiency ground data transmission

    NASA Technical Reports Server (NTRS)

    Dickinson, W. B.

    1973-01-01

    It is demonstrated that state-of-the-art communications technology can be implemented and reliably operated on a global basis to increase the transmission rates and efficiencies on circuits with bandwidths greater than the typical speech channel. Optimization is affected by optimum clock recovery procedures, multilevel pulse amplitude modulation, single sideband amplitude modulation, transversal filter equalizers, data scrambling, and active compensation for phase instability.

  19. Highly efficient gene transfer using a retroviral vector into murine T cells for preclinical chimeric antigen receptor-expressing T cell therapy

    SciTech Connect

    Kusabuka, Hotaka; Fujiwara, Kento; Tokunaga, Yusuke; Hirobe, Sachiko; Nakagawa, Shinsaku Okada, Naoki

    2016-04-22

    Adoptive immunotherapy using chimeric antigen receptor-expressing T (CAR-T) cells has attracted attention as an efficacious strategy for cancer treatment. To prove the efficacy and safety of CAR-T cell therapy, the elucidation of immunological mechanisms underlying it in mice is required. Although a retroviral vector (Rv) is mainly used for the introduction of CAR to murine T cells, gene transduction efficiency is generally less than 50%. The low transduction efficiency causes poor precision in the functional analysis of CAR-T cells. We attempted to improve the Rv gene transduction protocol to more efficiently generate functional CAR-T cells by optimizing the period of pre-cultivation and antibody stimulation. In the improved protocol, gene transduction efficiency to murine T cells was more than 90%. In addition, almost all of the prepared murine T cells expressed CAR after puromycin selection. These CAR-T cells had antigen-specific cytotoxic activity and secreted multiple cytokines by antigen stimulation. We believe that our optimized gene transduction protocol for murine T cells contributes to the advancement of T cell biology and development of immunotherapy using genetically engineered T cells. - Highlights: • We established highly efficient gene transduction protocols for murine T cells. • CD8{sup +} CAR-T cells had antigen-specific cytotoxic activity. • CD4{sup +} CAR-T cells secreted multiple cytokines by antigen stimulation. • This finding can contribute to the development of T-cell biology and immunotherapy.

  20. Highly Efficient Freestyle Magnetic Nanoswimmer.

    PubMed

    Li, Tianlong; Li, Jinxing; Morozov, Konstantin I; Wu, Zhiguang; Xu, Tailin; Rozen, Isaac; Leshansky, Alexander M; Li, Longqiu; Wang, Joseph

    2017-08-09

    The unique swimming strategies of natural microorganisms have inspired recent development of magnetic micro/nanorobots powered by artificial helical or flexible flagella. However, as artificial nanoswimmers with unique geometries are being developed, it is critical to explore new potential modes for kinetic optimization. For example, the freestyle stroke is the most efficient of the competitive swimming strokes for humans. Here we report a new type of magnetic nanorobot, a symmetric multilinked two-arm nanoswimmer, capable of efficient "freestyle" swimming at low Reynolds numbers. Excellent agreement between the experimental observations and theoretical predictions indicates that the powerful "freestyle" propulsion of the two-arm nanorobot is attributed to synchronized oscillatory deformations of the nanorobot under the combined action of magnetic field and viscous forces. It is demonstrated for the first time that the nonplanar propulsion gait due to the cooperative "freestyle" stroke of the two magnetic arms can be powered by a plane oscillatory magnetic field. These two-arm nanorobots are capable of a powerful propulsion up to 12 body lengths per second, along with on-demand speed regulation and remote navigation. Furthermore, the nonplanar propulsion gait powered by the consecutive swinging of the achiral magnetic arms is more efficient than that of common chiral nanohelical swimmers. This new swimming mechanism and its attractive performance opens new possibilities in designing remotely actuated nanorobots for biomedical operation at the nanoscale.

  1. High-performance spinning device for DVD-based micromechanical signal transduction

    NASA Astrophysics Data System (ADS)

    Hwu, En-Te; Chen, Ching-Hsiu; Bosco, Filippo G.; Wang, Wei-Min; Ko, Hsien-Chen; Hwang, Ing-Shouh; Boisen, Anja; Huang, Kuang-Yuh

    2013-04-01

    Here we report a high-throughput spinning device for nanometric scale measurements of microstructures with instrumentation details and experimental results. The readout technology implemented in the designed disc-like device is based on a DVD data storage optical pick-up unit (OPU). With a spinning mechanism, this device can simultaneously measure surface topography, mechanical deflections and resonance frequencies of several microfabricated beams at a high speed. In biochemical sensing applications, the OPU can measure bending changes of functionalized microcantilevers, providing a statistically robust and label-free bio-detection analysis of multiple compounds. The signal-to-noise ratio (S/N) is demonstrated from statistical measurements as 1.2 with arginine detection at 750 nM concentration. Practically, the OPU can measure up to 480 individual cantilever sensors per second with nanometer resolution. The opto-mechanical optimization of the device design and settings for biochemical detection are described.

  2. A low-voltage high-speed electronic switch based on piezoelectric transduction

    NASA Astrophysics Data System (ADS)

    Newns, Dennis; Elmegreen, Bruce; Hu Liu, Xiao; Martyna, Glenn

    2012-04-01

    We propose a novel digital switch, the piezoelectronic transistor or PET. Based on properties of known materials, we predict that a nanometer-scale PET can operate at low voltages and relatively high speeds, exceeding the capabilities of any conventional field effect transistor (FET). Depending on the degree to which these attributes can be simultaneously achieved, the device has a broad array of potential applications in digital logic. The PET is a 3-terminal switch in which a gate voltage is applied to a piezoelectric (PE), resulting in expansion compressing a piezoresistive (PR) material comprising the channel, which then undergoes a continuous, reversible insulator-metal transition. The channel becomes conducting in response to the gate voltage. A high piezoelectric coefficient PE, e.g., a relaxor piezoelectric, leads to low voltage operation. Suitable channel materials manifesting a pressure-induced metal-insulator transition can be found amongst rare earth chalcogenides, transition metal oxides, and among others. Mechanical requirements include a high PE/PR area ratio to step up pressure, a rigid surround material to constrain the PE and PR external boundaries normal to the strain axis, and a void space to enable free motion of the component side walls. Using static mechanical modeling and dynamic electro-acoustic simulations, we optimize device structure and materials and predict performance.

  3. Signal transduction pathways in erythrocyte nitric oxide metabolism under high fibrinogen levels

    NASA Astrophysics Data System (ADS)

    Saldanha, Carlota; Freitas, T.; Lopez de Almeida, J. P.; Silva-Herdade, A.

    2014-05-01

    Previous studies show that the fibrinogen molecule modulates the metabolism of nitric oxide (NO) in erythrocyte. The in vitro induced hiperfibrinogenemia interferes in the metabolism of the NO in the erythrocyte in dependence of the phosphorylation degree of the band 3. The soluble form of fibrinogen binds into CD47 protein present in the erythrocyte membrane. The soluble thrombomodulin is an inflammatory marker that binds to the erythrocyte CD47 in a site with a sequence peptide known as 4N1K. A study done in vitro shows that when hiperfibrinogenemia was induced in the presence of the peptide 4N1K agonist of CD47 it were observed variations in the efflux of NO from erythrocyte and an increase in the concentrations of GSNO, peroxinitrite, nitrite and nitrate of the erythrocytes. The aim of this work was to study the influence of the peptide 4N1K, on the metabolism of NO in the erythrocyte under high fibrinogen concentration and in the presence of inhibitors of the status of phosphorylation of protein band 3. In this in vitro study, whole blood samples were harvested from healthy subjects and NO, peroxynitrite, nitrite, nitrate and S-nitro-glutathione (GSNO) were determined in presence of 4N1K, calpeptine, Syk inhibitor and under high fibrinogen concentrations. The results obtained in erythrocytes under high fibrinogen levels when 4N1K is present with the Syk inhibitor or with calpeptine, showed in relation to the control samples increased significant concentrations of efflux of NO and of peroxynitrite, nitrite, nitrate and GSNO. In conclusion it was verified that in the in vitro model of hiperfibrinogenemia the peptide 4N1K, agonist of CD47, induces mobilization of NO in the erythrocyte in dependence of the status of phosphorylation of protein band 3.

  4. A polyimide-etalon thin film structure for all-optical high-frequency ultrasound transduction.

    PubMed

    Sheaff, Clay; Ashkenazi, Shai

    2012-10-01

    In this work, we have designed, fabricated, and tested an all-optical ultrasound transducer by integrating a photoabsorptive polyimide thin film into a Fabry-Pérot (etalon) high-frequency receiver. A 5-ns UV pulse was used for thermoelastic ultrasound generation in the polyimide film, and the transmission had a maximum amplitude of 4.3 MPa centered at 27 MHz with a fractional bandwidth of 107%. The device attained a noise-equivalent pressure of 1.3 Pa/√Hz in receive-only mode. When used in pulse-echo mode, the -6-dB upper cutoff frequency of the transmit/receive response reached 47 MHz. Basic imaging capabilities were also investigated by scanning the near-infrared probe beam across the device to create a 2 × 2 mm synthetic aperture. The imaging of targets placed at depths of 1.8 and 5.2 mm yielded estimates of 71 and 145 μm, respectively, for the lateral resolution and 35 and 38 μm, respectively, for the axial resolution. Finally, a design concept for a forward-viewing intravascular imager is presented that entails the coupling of light to a rotating, linear array of optical fibers on top of which are deposited polyimide-etalon transducers. Such a design would allow for a flexible and compact high-resolution imager well-suited for intravascular applications, such as guidance of treatment in the case of chronic total occlusion.

  5. High-efficiency solar concentrator

    NASA Technical Reports Server (NTRS)

    Lansing, F. L.; Dorman, J.

    1976-01-01

    A new type of solar concentrator is presented using liquid lenses and simple translational tracking mechanism. The concentrator achieves a 100:1 nominal concentration ratio and is compared in performance with a flat-plate collector having two sheets of glazing and non-selective coating. The results of the thermal analysis show that higher temperatures can be obtained with the concentrator than is possible with the non-concentrator flat-plate type. Furthermore, the thermal efficiency far exceeds that of the comparative flat-plate type for all operating conditions.

  6. High-efficiency solar concentrator

    NASA Technical Reports Server (NTRS)

    Lansing, F. L.; Dorman, J.

    1980-01-01

    A new type of solar concentrator is presented using liquid lenses and simple translational tracking mechanism. The concentrator achieves a 100:1 nominal concentration ratio and is compared in performance with a flat-plate collector having two sheets of glazing and non-selective coating. The results of the thermal analysis show that higher temperatures can be obtained with the concentrator than is possible with the non-concentrator flat-plate type. Furthermore, the thermal efficiency far exceeds that of the comparative flat-plate type for all operating conditions.

  7. High efficiency thermionic converter studies

    NASA Technical Reports Server (NTRS)

    Huffman, F. N.; Sommer, A. H.; Balestra, C. L.; Briere, T. R.; Lieb, D.; Oettinger, P. E.; Goodale, D. B.

    1977-01-01

    Research in thermionic energy conversion technology is reported. The objectives were to produce converters suitable for use in out of core space reactors, radioisotope generators, and solar satellites. The development of emitter electrodes that operate at low cesium pressure, stable low work function collector electrodes, and more efficient means of space charge neutralization were investigated to improve thermionic converter performance. Potential improvements in collector properties were noted with evaporated thin film barium oxide coatings. Experiments with cesium carbonate suggest this substance may provide optimum combinations of cesium and oxygen for thermionic conversion.

  8. Highly specific electronic signal transduction mediated by DNA/metal self-assembly.

    SciTech Connect

    Dentinger, Paul M.; Pathak, Srikant

    2003-11-01

    Highly specific interactions between DNA could potentially be amplified if the DNA interactions were utilized to assemble large scale parts. Fluidic assembly of microsystem parts has the potential for rapid and accurate placement of otherwise difficult to handle pieces. Ideally, each part would have a different chemical interaction that allowed it to interact with the substrate only in specific areas. One easy way to obtain a multiple chemical permutations is to use synthetic DNA oligomers. Si parts were prepared using silicon-on-insulator technology microfabrication techniques. Several surface chemistry protocols were developed to react commercial oligonucleotides to the parts. However, no obvious assembly was achieved. It was thought that small defects on the surface did not allow the microparts to be in close enough proximity for DNA hybridization, and this was. in part, confirmed by interferometry. To assist in the hybridization, plastic, pliable parts were manufactured and a new chemistry was developed. However, assembly was still absent even with the application of force. It is presently thought that one of three mechanisms is preventing the assembly. The surfaces of the two solid substrates can not get in close enough proximity, the surface chemistry lacks sufficient density to keep the parts from separating, or DNA interactions in close proximity on solid substrates are forbidden. These possibilities are discussed in detail.

  9. High energy efficient solid state laser sources

    NASA Technical Reports Server (NTRS)

    Byer, Robert L.

    1989-01-01

    Recent progress in the development of highly efficient coherent optical sources was reviewed. This work has focused on nonlinear frequency conversion of the highly coherent output of the non-planar ring laser oscillators developed earlier in the program, and includes high efficiency second harmonic generation and the operation of optical parametric oscillators for wavelength diversity and tunability.

  10. High energy efficient solid state laser sources

    NASA Technical Reports Server (NTRS)

    Byer, Robert L.

    1988-01-01

    Recent progress in the development of highly efficient coherent optical sources is reviewed. This work focusses on nonlinear frequency conversion of the highly coherent output of the Non-Planar Ring Laser Oscillators developed earlier in the program, and includes high efficiency second harmonic generation and the operation of optical parametric oscillators for wavelength diversity and tunability.

  11. Efficient high density train operations

    DOEpatents

    Gordon, Susanna P.; Evans, John A.

    2001-01-01

    The present invention provides methods for preventing low train voltages and managing interference, thereby improving the efficiency, reliability, and passenger comfort associated with commuter trains. An algorithm implementing neural network technology is used to predict low voltages before they occur. Once voltages are predicted, then multiple trains can be controlled to prevent low voltage events. Further, algorithms for managing inference are presented in the present invention. Different types of interference problems are addressed in the present invention such as "Interference. During Acceleration", "Interference Near Station Stops", and "Interference During Delay Recovery." Managing such interference avoids unnecessary brake/acceleration cycles during acceleration, immediately before station stops, and after substantial delays. Algorithms are demonstrated to avoid oscillatory brake/acceleration cycles due to interference and to smooth the trajectories of closely following trains. This is achieved by maintaining sufficient following distances to avoid unnecessary braking/accelerating. These methods generate smooth train trajectories, making for a more comfortable ride, and improve train motor reliability by avoiding unnecessary mode-changes between propulsion and braking. These algorithms can also have a favorable impact on traction power system requirements and energy consumption.

  12. High Efficiency Thermoelectric Generator: Integration

    DTIC Science & Technology

    2011-02-25

    included: − material barriers such as thermal blankets, glass bubbles and aerogels , − encapsulation with high molecular weight gases (e.g. Xenon... aerogels impregnated with radiation scattering particles (investigated at the thermoelectric group in the NASA Jet Propulsion Laboratory). Thrust

  13. High efficiency solar photovoltaic power module concept

    NASA Technical Reports Server (NTRS)

    Bekey, I.

    1978-01-01

    The investigation of a preliminary concept for high efficiency solar power generation in space is presented. The concept was a synergistic combination of spectral splitting, tailored bandgap cells, high concentration ratios, and cool cell areas.

  14. High-Efficiency Power Module

    NASA Technical Reports Server (NTRS)

    Simons, Rainee N. (Inventor); Wintucky, Edwin G. (Inventor)

    2015-01-01

    One or more embodiments of the present invention pertain to an all solid-state microwave power module. The module includes a plurality of solid-state amplifiers configured to amplify a signal using a low power stage, a medium power stage, and a high power stage. The module also includes a power conditioner configured to activate a voltage sequencer (e.g., bias controller) when power is received from a power source. The voltage sequencer is configured to sequentially apply voltage to a gate of each amplifier and sequentially apply voltage to a drain of each amplifier.

  15. High-Efficiency Power Module

    NASA Technical Reports Server (NTRS)

    Simons, Rainee N (Inventor); Wintucky, Edwin G (Inventor)

    2013-01-01

    One or more embodiments of the present invention pertain to an all solid-state microwave power module. The module includes a plurality of solid-state amplifiers configured to amplify a signal using a low power stage, a medium power stage, and a high power stage. The module also includes a power conditioner configured to activate a voltage sequencer (e.g., bias controller) when power is received from a power source. The voltage sequencer is configured to sequentially apply voltage to a gate of each amplifier and sequentially apply voltage to a drain of each amplifier.

  16. High Efficiency Microwave Power Amplifier (HEMPA) Design

    NASA Technical Reports Server (NTRS)

    Sims, W. Herbert

    2004-01-01

    This paper will focus on developing an exotic switching technique that enhances the DC-to-RF conversion efficiency of microwave power amplifiers. For years, switching techniques implemented in the 10 kHz to 30 MHz region have resulted in DC-to-RF conversion efficiencies of 90-95-percent. Currently amplifier conversion efficiency, in the 2-3 GHz region approaches, 10-20-percent. Using a combination of analytical modeling and hardware testing, a High Efficiency Microwave Power Amplifier was built that demonstrated conversion efficiencies four to five times higher than current state of the art.

  17. Multicolor, High Efficiency, Nanotextured LEDs

    SciTech Connect

    Jung Han; Arto Nurmikko

    2011-09-30

    We report on research results in this project which synergize advanced material science approaches with fundamental optical physics concepts pertaining to light-matter interaction, with the goal of solving seminal problems for the development of very high performance light emitting diodes (LEDs) in the blue and green for Solid State Lighting applications. Accomplishments in the duration of the contract period include (i) heteroepitaxy of nitrogen-polar LEDs on sapphire, (ii) heteroepitaxy of semipolar (11{bar 2}2) green LEDs on sapphire, (iii) synthesis of quantum-dot loaded nanoporous GaN that emits white light without phosphor conversion, (iv) demonstration of the highest quality semipolar (11{bar 2}2) GaN on sapphire using orientation-controlled epitaxy, (v) synthesis of nanoscale GaN and InGaN medium, and (vi) development of a novel liftoff process for manufacturing GaN thin-film vertical LEDs. The body of results is presented in this report shows how a solid foundation has been laid, with several noticeable accomplishments, for innovative research, consistent with the stated milestones.

  18. High-Efficiency Autonomous Coherent Lidar

    NASA Technical Reports Server (NTRS)

    Gatt, Philip; Henderson, Sammy W.; Hannon, Stephen M.

    1999-01-01

    A useful measure of sensor performance is the transceiver system efficiency n (sub sys). Which consists of the antenna efficiency n (sub a) and optical and electronic losses. Typically, the lidar equation and the antenna efficiency are defined in terms of the telescope aperture area. However, during the assembly of a coherent transceiver, it is important to measure the system efficiency before the installation of the beamexpanding telescope (i.e., the untruncated-beam system efficiency). Therefore, to accommodate both truncated and untruncated beam efficiency measurements, we define the lidar equation and the antenna efficiency in terms of the beam area rather than the commonly used aperture area referenced definition. With a well-designed Gaussian-beam lidar, aperture area referenced system efficiencies of 15 to 20 % (23-31% relative to the beam area) are readily achievable. In this paper we compare the differences between these efficiency definitions. We then describe techniques by which high efficiency can be achieved, followed by a discussion several novel auto alignment techniques developed to maintain high efficiency.

  19. Cell-penetrating peptides: Possible transduction mechanisms and therapeutic applications

    PubMed Central

    GUO, ZHENGRONG; PENG, HUANYAN; KANG, JIWEN; SUN, DIANXING

    2016-01-01

    Cell-penetrating peptides (CPPs), also known as protein transduction domains, are a class of diverse peptides with 5–30 amino acids. CPPs are divided into cationic, amphipathic and hydrophobic CPPs. They are able to carry small molecules, plasmid DNA, small interfering RNA, proteins, viruses, imaging agents and other various nanoparticles across the cellular membrane, resulting in internalization of the intact cargos. However, the mechanisms of CPP internalization remain to be elucidated. Recently, CPPs have received considerable attention due to their high transduction efficiency and low cytotoxicity. These peptides have a significant potential for diagnostic and therapeutic applications, such as delivery of fluorescent or radioactive compounds for imaging, delivery of peptides and proteins for therapeutic application, and delivery of molecules into induced pluripotent stem cells for directing differentiation. The present study reviews the classifications and transduction mechanisms of CPPs, as well as their potential applications. PMID:27123243

  20. High Efficiency Lithium-Thionyl Chloride Cell.

    DTIC Science & Technology

    1982-04-01

    AD-Al14 672 HONEYWELL POWER SOURCES CENTER HORSHAM PA F/S 10/3 HIGH EFFICIENCY LITHIUM - THIONYL CHLORIDE CELLo(U) APR 82 N DODDAPANEN! OAAK20-81-C...CHART NATIONAl BUREAU OF STANDARDS 1963 A Research and Development Technical Report DELET-TR-81-0381-3 HIGH EFFICIENCY LITHIUM - THIONYL CHLORIDE CELL...reverse aide it necessary and Identify by block number) Thionyl chloride , lithium , high discharge rates, low temperatures, catalysis, cyclic

  1. Highly-efficient high-power pumps for fiber lasers

    NASA Astrophysics Data System (ADS)

    Gapontsev, V.; Moshegov, N.; Berezin, I.; Komissarov, A.; Trubenko, P.; Miftakhutdinov, D.; Berishev, I.; Chuyanov, V.; Raisky, O.; Ovtchinnikov, A.

    2017-02-01

    We report on high efficiency multimode pumps that enable ultra-high efficiency high power ECO Fiber Lasers. We discuss chip and packaged pump design and performance. Peak out-of-fiber power efficiency of ECO Fiber Laser pumps was reported to be as high as 68% and was achieved with passive cooling. For applications that do not require Fiber Lasers with ultimate power efficiency, we have developed passively cooled pumps with out-of-fiber power efficiency greater than 50%, maintained at operating current up to 22A. We report on approaches to diode chip and packaged pump design that possess such performance.

  2. Molecular profiling reveals diversity of stress signal transduction cascades in highly penetrant Alzheimer's disease human skin fibroblasts.

    PubMed

    Mendonsa, Graziella; Dobrowolska, Justyna; Lin, Angela; Vijairania, Pooja; Jong, Y-J I; Baenziger, Nancy L

    2009-01-01

    The serious and growing impact of the neurodegenerative disorder Alzheimer's disease (AD) as an individual and societal burden raises a number of key questions: Can a blanket test for Alzheimer's disease be devised forecasting long-term risk for acquiring this disorder? Can a unified therapy be devised to forestall the development of AD as well as improve the lot of present sufferers? Inflammatory and oxidative stresses are associated with enhanced risk for AD. Can an AD molecular signature be identified in signaling pathways for communication within and among cells during inflammatory and oxidative stress, suggesting possible biomarkers and therapeutic avenues? We postulated a unique molecular signature of dysfunctional activity profiles in AD-relevant signaling pathways in peripheral tissues, based on a gain of function in G-protein-coupled bradykinin B2 receptor (BKB2R) inflammatory stress signaling in skin fibroblasts from AD patients that results in tau protein Ser hyperphosphorylation. Such a signaling profile, routed through both phosphorylation and proteolytic cascades activated by inflammatory and oxidative stresses in highly penetrant familial monogenic forms of AD, could be informative for pathogenesis of the complex multigenic sporadic form of AD. Comparing stimulus-specific cascades of signal transduction revealed a striking diversity of molecular signaling profiles in AD human skin fibroblasts that express endogenous levels of mutant presenilins PS-1 or PS-2 or the Trisomy 21 proteome. AD fibroblasts bearing the PS-1 M146L mutation associated with highly aggressive AD displayed persistent BKB2R signaling plus decreased ERK activation by BK, correctible by gamma-secretase inhibitor Compound E. Lack of these effects in the homologous PS-2 mutant cells indicates specificity of presenilin gamma-secretase catalytic components in BK signaling biology directed toward MAPK activation. Oxidative stress revealed a JNK-dependent survival pathway in normal

  3. Molecular Profiling Reveals Diversity of Stress Signal Transduction Cascades in Highly Penetrant Alzheimer's Disease Human Skin Fibroblasts

    PubMed Central

    Mendonsa, Graziella; Dobrowolska, Justyna; Lin, Angela; Vijairania, Pooja; Jong, Y.-J. I.; Baenziger, Nancy L.

    2009-01-01

    The serious and growing impact of the neurodegenerative disorder Alzheimer's disease (AD) as an individual and societal burden raises a number of key questions: Can a blanket test for Alzheimer's disease be devised forecasting long-term risk for acquiring this disorder? Can a unified therapy be devised to forestall the development of AD as well as improve the lot of present sufferers? Inflammatory and oxidative stresses are associated with enhanced risk for AD. Can an AD molecular signature be identified in signaling pathways for communication within and among cells during inflammatory and oxidative stress, suggesting possible biomarkers and therapeutic avenues? We postulated a unique molecular signature of dysfunctional activity profiles in AD-relevant signaling pathways in peripheral tissues, based on a gain of function in G-protein-coupled bradykinin B2 receptor (BKB2R) inflammatory stress signaling in skin fibroblasts from AD patients that results in tau protein Ser hyperphosphorylation. Such a signaling profile, routed through both phosphorylation and proteolytic cascades activated by inflammatory and oxidative stresses in highly penetrant familial monogenic forms of AD, could be informative for pathogenesis of the complex multigenic sporadic form of AD. Comparing stimulus-specific cascades of signal transduction revealed a striking diversity of molecular signaling profiles in AD human skin fibroblasts that express endogenous levels of mutant presenilins PS-1 or PS-2 or the Trisomy 21 proteome. AD fibroblasts bearing the PS-1 M146L mutation associated with highly aggressive AD displayed persistent BKB2R signaling plus decreased ERK activation by BK, correctible by gamma-secretase inhibitor Compound E. Lack of these effects in the homologous PS-2 mutant cells indicates specificity of presenilin gamma-secretase catalytic components in BK signaling biology directed toward MAPK activation. Oxidative stress revealed a JNK-dependent survival pathway in normal

  4. Early transduction produces highly functional chimeric antigen receptor-modified virus-specific T-cells with central memory markers: a Production Assistant for Cell Therapy (PACT) translational application.

    PubMed

    Sun, Jiali; Huye, Leslie E; Lapteva, Natalia; Mamonkin, Maksim; Hiregange, Manasa; Ballard, Brandon; Dakhova, Olga; Raghavan, Darshana; Durett, April G; Perna, Serena K; Omer, Bilal; Rollins, Lisa A; Leen, Ann M; Vera, Juan F; Dotti, Gianpietro; Gee, Adrian P; Brenner, Malcolm K; Myers, Douglas G; Rooney, Cliona M

    2015-01-01

    Virus-specific T-cells (VSTs) proliferate exponentially after adoptive transfer into hematopoietic stem cell transplant (HSCT) recipients, eliminate virus infections, then persist and provide long-term protection from viral disease. If VSTs behaved similarly when modified with tumor-specific chimeric antigen receptors (CARs), they should have potent anti-tumor activity. This theory was evaluated by Cruz et al. in a previous clinical trial with CD19.CAR-modified VSTs, but there was little apparent expansion of these cells in patients. In that study, VSTs were gene-modified on day 19 of culture and we hypothesized that by this time, sufficient T-cell differentiation may have occurred to limit the subsequent proliferative capacity of the transduced T-cells. To facilitate the clinical testing of this hypothesis in a project supported by the NHLBI-PACT mechanism, we developed and optimized a good manufacturing practices (GMP) compliant method for the early transduction of VSTs directed to Epstein-Barr virus (EBV), Adenovirus (AdV) and cytomegalovirus (CMV) using a CAR directed to the tumor-associated antigen disialoganglioside (GD2). Ad-CMVpp65-transduced EBV-LCLs effectively stimulated VSTs directed to all three viruses (triVSTs). Transduction efficiency on day three was increased in the presence of cytokines and high-speed centrifugation of retroviral supernatant onto retronectin-coated plates, so that under optimal conditions up to 88% of tetramer-positive VSTs expressed the GD2.CAR. The average transduction efficiency of early-and late transduced VSTs was 55 ± 4% and 22 ± 5% respectively, and early-transduced VSTs maintained higher frequencies of T cells with central memory or intermediate memory phenotypes. Early-transduced VSTs also had higher proliferative capacity and produced higher levels of TH1 cytokines IL-2, TNF-α, IFN-γ, MIP-1α, MIP-1β and other cytokines in vitro. We developed a rapid and GMP compliant method for the early transduction of

  5. Auxin signal transduction.

    PubMed

    Hagen, Gretchen

    2015-01-01

    The plant hormone auxin (indole-3-acetic acid, IAA) controls growth and developmental responses throughout the life of a plant. A combination of molecular, genetic and biochemical approaches has identified several key components involved in auxin signal transduction. Rapid auxin responses in the nucleus include transcriptional activation of auxin-regulated genes and degradation of transcriptional repressor proteins. The nuclear auxin receptor is an integral component of the protein degradation machinery. Although auxin signalling in the nucleus appears to be short and simple, recent studies indicate that there is a high degree of diversity and complexity, largely due to the existence of multigene families for each of the major molecular components. Current studies are attempting to identify interacting partners among these families, and to define the molecular mechanisms involved in the interactions. Future goals are to determine the levels of regulation of the key components of the transcriptional complex, to identify higher-order complexes and to integrate this pathway with other auxin signal transduction pathways, such as the pathway that is activated by auxin binding to a different receptor at the outer surface of the plasma membrane. In this case, auxin binding triggers a signal cascade that affects a number of rapid cytoplasmic responses. Details of this pathway are currently under investigation. © 2015 Authors; published by Portland Press Limited.

  6. Very High Efficiency Solar Cell Modules

    SciTech Connect

    Barnett, A.; Kirkpatrick, D.; Honsberg, C.; Moore, D.; Wanlass, M.; Emery, K.; Schwartz, R.; Carlson, D.; Bowden, S.; Aiken, D.; Gray, A.; Kurtz, S.; Kazmerski, L., et al

    2009-01-01

    The Very High Efficiency Solar Cell (VHESC) program is developing integrated optical system - PV modules for portable applications that operate at greater than 50% efficiency. We are integrating the optical design with the solar cell design, and have entered previously unoccupied design space. Our approach is driven by proven quantitative models for the solar cell design, the optical design, and the integration of these designs. Optical systems efficiency with an optical efficiency of 93% and solar cell device results under ideal dichroic splitting optics summing to 42.7 {+-} 2.5% are described.

  7. High efficiency flat plate solar energy collector

    SciTech Connect

    Butler, R. F.

    1985-04-30

    A concentrating flat plate collector for the high efficiency collection of solar energy. Through an arrangement of reflector elements, incoming solar radiation, either directly or after reflection from the reflector elements, impinges upon both surfaces of a collector element.

  8. Multi Band Gap High Efficiency Converter (RAINBOW)

    NASA Technical Reports Server (NTRS)

    Bekey, I.; Lewis, C.; Phillips, W.; Shields, V.; Stella, P.

    1997-01-01

    The RAINBOW multi band gap system represents a unique combination of solar cells, concentrators and beam splitters. RAINBOW is a flexible system which can readily expand as new high efficiency components are developed.

  9. Junior High Gets Energy Efficient VAV System

    ERIC Educational Resources Information Center

    Modern Schools, 1977

    1977-01-01

    Minnesota's Isanti Junior High, designed with an energy efficient variable air volume system, is an innovative school selected for display at the 1977 Exhibition of School Architecture in Las Vegas. (Author/MLF)

  10. High-efficiency silicon solar cell research

    NASA Technical Reports Server (NTRS)

    Daud, T.

    1984-01-01

    Progress reports on research in high-efficiency silicon solar cells were presented by eight contractors and JPL. The presentations covered the issues of Bulk and Surface Loss, Modeling, Measurements, and Proof of Concept.

  11. Polyploidization without mitosis improves in vivo liver transduction with lentiviral vectors.

    PubMed

    Pichard, Virginie; Couton, Dominique; Desdouets, Chantal; Ferry, Nicolas

    2013-02-01

    Lentiviral vectors are efficient gene delivery vehicles for therapeutic and research applications. In contrast to oncoretroviral vectors, they are able to infect most nonproliferating cells. In the liver, induction of cell proliferation dramatically improved hepatocyte transduction using all types of retroviral vectors. However, the precise relationship between hepatocyte division and transduction efficiency has not been determined yet. Here we compared gene transfer efficiency in the liver after in vivo injection of recombinant lentiviral or Moloney murine leukemia viral (MoMuLV) vectors in hepatectomized rats treated or not with retrorsine, an alkaloid that blocks hepatocyte division and induces megalocytosis. Partial hepatectomy alone resulted in a similar increase in hepatocyte transduction using either vector. In retrorsine-treated and partially hepatectomized rats, transduction with MoMuLV vectors dropped dramatically. In contrast, we observed that retrorsine treatment combined with partial hepatectomy increased lentiviral transduction to higher levels than hepatectomy alone. Analysis of nuclear ploidy in single cells showed that a high level of transduction was associated with polyploidization. In conclusion, endoreplication could be exploited to improve the efficiency of liver-directed lentiviral gene therapy.

  12. High efficiency quantum cascade laser frequency comb

    PubMed Central

    Lu, Quanyong; Wu, Donghai; Slivken, Steven; Razeghi, Manijeh

    2017-01-01

    An efficient mid-infrared frequency comb source is of great interest to high speed, high resolution spectroscopy and metrology. Here we demonstrate a mid-IR quantum cascade laser frequency comb with a high power output and narrow beatnote linewidth at room temperature. The active region was designed with a strong-coupling between the injector and the upper lasing level for high internal quantum efficiency and a broadband gain. The group velocity dispersion was engineered for efficient, broadband mode-locking via four wave mixing. The comb device exhibits a narrow intermode beatnote linewidth of 50.5 Hz and a maximum wall-plug efficiency of 6.5% covering a spectral coverage of 110 cm−1 at λ ~ 8 μm. The efficiency is improved by a factor of 6 compared with previous demonstrations. The high power efficiency and narrow beatnote linewidth will greatly expand the applications of quantum cascade laser frequency combs including high-precision remote sensing and spectroscopy. PMID:28262834

  13. High efficiency quantum cascade laser frequency comb

    NASA Astrophysics Data System (ADS)

    Lu, Quanyong; Wu, Donghai; Slivken, Steven; Razeghi, Manijeh

    2017-03-01

    An efficient mid-infrared frequency comb source is of great interest to high speed, high resolution spectroscopy and metrology. Here we demonstrate a mid-IR quantum cascade laser frequency comb with a high power output and narrow beatnote linewidth at room temperature. The active region was designed with a strong-coupling between the injector and the upper lasing level for high internal quantum efficiency and a broadband gain. The group velocity dispersion was engineered for efficient, broadband mode-locking via four wave mixing. The comb device exhibits a narrow intermode beatnote linewidth of 50.5 Hz and a maximum wall-plug efficiency of 6.5% covering a spectral coverage of 110 cm‑1 at λ ~ 8 μm. The efficiency is improved by a factor of 6 compared with previous demonstrations. The high power efficiency and narrow beatnote linewidth will greatly expand the applications of quantum cascade laser frequency combs including high-precision remote sensing and spectroscopy.

  14. High efficiency quantum cascade laser frequency comb.

    PubMed

    Lu, Quanyong; Wu, Donghai; Slivken, Steven; Razeghi, Manijeh

    2017-03-06

    An efficient mid-infrared frequency comb source is of great interest to high speed, high resolution spectroscopy and metrology. Here we demonstrate a mid-IR quantum cascade laser frequency comb with a high power output and narrow beatnote linewidth at room temperature. The active region was designed with a strong-coupling between the injector and the upper lasing level for high internal quantum efficiency and a broadband gain. The group velocity dispersion was engineered for efficient, broadband mode-locking via four wave mixing. The comb device exhibits a narrow intermode beatnote linewidth of 50.5 Hz and a maximum wall-plug efficiency of 6.5% covering a spectral coverage of 110 cm(-1) at λ ~ 8 μm. The efficiency is improved by a factor of 6 compared with previous demonstrations. The high power efficiency and narrow beatnote linewidth will greatly expand the applications of quantum cascade laser frequency combs including high-precision remote sensing and spectroscopy.

  15. Important loss mechanisms in high-efficiency solar cells

    NASA Technical Reports Server (NTRS)

    Sah, C. T.

    1984-01-01

    A study was conducted to identify loss mechanisms in high efficiency silicon solar cells. The following were considered: (1) recombination loss mechanisms; (2) high efficiency cells; (3) very high efficiency cells; and (4) ultra high efficiency cells.

  16. High-efficiency silicon solar cells

    NASA Technical Reports Server (NTRS)

    Green, M. A.; Blakers, A. W.; Shi, J.; Keller, E. M.; Wenham, S. R.

    1984-01-01

    Silicon solar cells are described which operate at energy conversion efficiencies independently measured at 18.7 percent under standard terrestrial test conditions (AM1.5, 100 mW/sq cm, 28 C). These are apparently the most efficient silicon cells fabricated to date. The high-efficiency results from a combination of high open-circuit voltage due to the careful attention paid to the passivation of the top surface of the cell, high fill factor due to the high open-circuit voltage and low parasitic resistance losses, and high short-circuit current density due to the use of shallow diffusions, a low grid coverage, and an optimized double layer antireflection coating.

  17. Bacteriophage Transduction in Staphylococcus epidermidis

    PubMed Central

    Olson, Michael E.; Horswill, Alexander R.

    2016-01-01

    The genetic manipulation of Staphylococcus epidermidis for molecular experimentation has long been an area of difficulty. Many of the traditional laboratory techniques for strain construction are laborious and hampered by poor efficiency. The ability to move chromosomal genetic markers and plasmids using bacteriophage transduction has greatly increased the speed and ease of S. epidermidis studies. These molecular genetic advances have advanced the S. epidermidis research field beyond a select few genetically tractable strains and facilitated investigations of clinically relevant isolates. PMID:24222465

  18. Microfluidic chip for high efficiency DNA extraction.

    PubMed

    Chung, Yung-Chiang; Jan, Ming-Shiung; Lin, Yu-Cheng; Lin, Ju-Hwa; Cheng, Wang-Chin; Fan, Chia-Yu

    2004-04-01

    A high efficiency DNA extraction microchip was designed to extract DNA from lysed cells using immobilized beads and the solution flowing back and forth. This chip was able to increase the extraction efficiency by 2-fold when there was no serum. When serum existed in the solution, the extraction efficiency of immobilized beads was 88-fold higher than that of free beads. The extraction efficiency of the microchip was tested under different conditions and numbers of E. coli cells. When the number of E. coli cells was between 10(6) and 10(8) in 25 microl of whole blood, the extraction efficiency using immobilized beads was only slightly higher than that using free beads (10(0) to 10(1) fold). When the number of E. coli cells was in the range 10(4) to 10(6) in 25 microl of whole blood, the extraction efficiency of immobilized beads was greater than that of the free beads (10(1) to 10(2) fold). When the number of E. coli cells was lower, in the range 10(3) to 10(4) in 25 microl of whole blood, the extraction efficiency of immobilized beads was much higher than that of the free beads (10(2) to 10(3) fold). This study indicated that DNA could be efficiently extracted even when the number of bacterial cells was smaller (10(5) to 10(3)). This microfluidic extraction chip could find potential applications in rare sample genomic study.

  19. The future of high efficiency solar cells

    NASA Technical Reports Server (NTRS)

    Fan, J. C. C.

    1984-01-01

    Research approaches to obtain solar cell modules with 1 sun efficiencies of 20-30 percent at air mass 1 are now well understood. Such high efficiency modules should become available in the near future. It can be expected that these modules will be extensively used in terrestrial power generation, space power generation, and consumer electronics. To achieve practical module efficiencies significantly above 30 percent, it will be necessary to employ concepts other than spectral splitting, such as spectral compression and broad band detection. A major breakthrough in these areas is not anticipated at this time.

  20. High efficiency wraparound contact solar cells /HEWACS/

    NASA Technical Reports Server (NTRS)

    Gillanders, M.; Opjorden, R.

    1980-01-01

    A cell technology, producing high efficiency wrap-around contact solar cells (HEWACS), with both electrical contacts on the back and AMO conversion efficiencies of almost 15%, is presented. A flow chart indicating the baseline process sequence along with the process changes is given. Tests checking for coating delamination and contact integrity, those measuring contact strength, and thermal cycle tests, successfully demonstrated that this cell technology is ready to be moved to the pilot production stage.

  1. Efficient synthesis of highly substituted tetrahydroindazolone derivatives.

    PubMed

    Scala, Angela; Piperno, Anna; Risitano, Francesco; Cirmi, Santa; Navarra, Michele; Grassi, Giovanni

    2015-08-01

    A straightforward and efficient method for the synthesis of novel highly substituted and diversely functionalized indazolone derivatives has been developed. The transformation consists of a cyclocondensation of selected 1,3,3'-tricarbonyls with monosubstituted hydrazines. The starting β-triketones were prepared by an efficient chemo- and regioselective method under MW irradiation, exploiting the oxazolone chemistry. The reaction is easily accomplished under mild conditions and appears versatile, providing a synthetic diversification method with potential for drug-like compounds preparation.

  2. Query by transduction.

    PubMed

    Ho, Shen-Shyang; Wechsler, Harry

    2008-09-01

    There has been recently a growing interest in the use of transductive inference for learning. We expand here the scope of transductive inference to active learning in a stream-based setting. Towards that end this paper proposes Query-by-Transduction (QBT) as a novel active learning algorithm. QBT queries the label of an example based on the p-values obtained using transduction. We show that QBT is closely related to Query-by-Committee (QBC) using relations between transduction, Bayesian statistical testing, Kullback-Leibler divergence, and Shannon information. The feasibility and utility of QBT is shown on both binary and multi-class classification tasks using SVM as the choice classifier. Our experimental results show that QBT compares favorably, in terms of mean generalization, against random sampling, committee-based active learning, margin-based active learning, and QBC in the stream-based setting.

  3. On the calculation of signal transduction ability of signaling transduction pathways in intracellular communication: systematic approach.

    PubMed

    Chen, Bor-Sen; Wu, Chia-Chou

    2012-06-15

    The major function of signal transduction pathways in cells is to sense signals from the environment and process the information through signaling molecules in order to regulate the activity of transcription factors. On the molecular level, the information transmitted by a small number of signal molecules is amplified in the internal signaling pathway through enzyme catalysis, molecular modification and via the activation or inhibition of interactions. However, the dynamic system behavior of a signaling pathway can be complex and, despite knowledge of the pathway components and interactions, it is still a challenge to interpret the pathways behavior. Therefore, a systematic method is proposed in this study to quantify the signal transduction ability. Based on the non-linear signal transduction system, signal transduction ability can be investigated by solving a Hamilton-Jacobi inequality (HJI)-constrained optimization problem. To avoid difficulties associated with solving a complex HJI-constrained optimization problem for signal transduction ability, the Takagi-Sugeno fuzzy model is introduced to approximate the non-linear signal transduction system by interpolating several local linear systems so that the HJI-constrained optimization problem can be replaced by a linear matrix inequality (LMI)-constrained optimization problem. The LMI problem can then be efficiently solved for measuring signal transduction ability. Finally, the signal transduction ability of two important signal transduction pathways was measured by the proposed method and confirmed using experimental data, which is useful for biotechnological and therapeutic application and drug design.

  4. High-efficiency solid state power amplifier

    NASA Technical Reports Server (NTRS)

    Wallis, Robert E. (Inventor); Cheng, Sheng (Inventor)

    2005-01-01

    A high-efficiency solid state power amplifier (SSPA) for specific use in a spacecraft is provided. The SSPA has a mass of less than 850 g and includes two different X-band power amplifier sections, i.e., a lumped power amplifier with a single 11-W output and a distributed power amplifier with eight 2.75-W outputs. These two amplifier sections provide output power that is scalable from 11 to 15 watts without major design changes. Five different hybrid microcircuits, including high-efficiency Heterostructure Field Effect Transistor (HFET) amplifiers and Monolithic Microwave Integrated Circuit (MMIC) phase shifters have been developed for use within the SSPA. A highly efficient packaging approach enables the integration of a large number of hybrid circuits into the SSPA.

  5. Technology Development for High Efficiency Optical Communications

    NASA Technical Reports Server (NTRS)

    Farr, William H.

    2012-01-01

    Deep space optical communications is a significantly more challenging operational domain than near Earth space optical communications, primarily due to effects resulting from the vastly increased range between transmitter and receiver. The NASA Game Changing Development Program Deep Space Optical Communications Project is developing four key technologies for the implementation of a high efficiency telecommunications system that will enable greater than 10X the data rate of a state-of-the-art deep space RF system (Ka-band) for similar transceiver mass and power burden on the spacecraft. These technologies are a low mass spacecraft disturbance isolation assembly, a flight qualified photon counting detector array, a high efficiency flight laser amplifier and a high efficiency photon counting detector array for the ground-based receiver.

  6. Measure Guideline. High Efficiency Natural Gas Furnaces

    SciTech Connect

    Brand, L.; Rose, W.

    2012-10-01

    This measure guideline covers installation of high-efficiency gas furnaces, including: when to install a high-efficiency gas furnace as a retrofit measure; how to identify and address risks; and the steps to be used in the selection and installation process. The guideline is written for Building America practitioners and HVAC contractors and installers. It includes a compilation of information provided by manufacturers, researchers, and the Department of Energy as well as recent research results from the Partnership for Advanced Residential Retrofit (PARR) Building America team.

  7. Measure Guideline: High Efficiency Natural Gas Furnaces

    SciTech Connect

    Brand, L.; Rose, W.

    2012-10-01

    This Measure Guideline covers installation of high-efficiency gas furnaces. Topics covered include when to install a high-efficiency gas furnace as a retrofit measure, how to identify and address risks, and the steps to be used in the selection and installation process. The guideline is written for Building America practitioners and HVAC contractors and installers. It includes a compilation of information provided by manufacturers, researchers, and the Department of Energy as well as recent research results from the Partnership for Advanced Residential Retrofit (PARR) Building America team.

  8. Highly efficient heralding of entangled single photons.

    PubMed

    Ramelow, Sven; Mech, Alexandra; Giustina, Marissa; Gröblacher, Simon; Wieczorek, Witlef; Beyer, Jörn; Lita, Adriana; Calkins, Brice; Gerrits, Thomas; Nam, Sae Woo; Zeilinger, Anton; Ursin, Rupert

    2013-03-25

    Single photons are an important prerequisite for a broad spectrum of quantum optical applications. We experimentally demonstrate a heralded single-photon source based on spontaneous parametric down-conversion in collinear bulk optics, and fiber-coupled bolometric transition-edge sensors. Without correcting for background, losses, or detection inefficiencies, we measure an overall heralding efficiency of 83%. By violating a Bell inequality, we confirm the single-photon character and high-quality entanglement of our heralded single photons which, in combination with the high heralding efficiency, are a necessary ingredient for advanced quantum communication protocols such as one-sided device-independent quantum key distribution.

  9. Interface modification for highly efficient organic photovoltaics

    NASA Astrophysics Data System (ADS)

    Steim, Roland; Choulis, Stelios A.; Schilinsky, Pavel; Brabec, Christoph J.

    2008-03-01

    We present highly efficient inverted polymer:fullerene bulk-heterojunction solar cells by incorporation of a nanoscale organic interfacial layer between the indium tin oxide (ITO) and the metal oxide electron-conducting layer. We demonstrate that stacking of solution-processed organic and metal oxide interfacial layers gives highly charged selective low ohmic cathodes. The incorporation of a polyoxyethylene tridecyl ether interfacial layer between ITO and solution-processed titanium oxide (TiOx) raised the power conversion efficiency of inverted organic photovoltaics to 3.6%, an improvement of around 15% in their performance over comparable devices without the organic interfacial layer.

  10. Proposal for superstructure based high efficiency photovoltaics

    NASA Technical Reports Server (NTRS)

    Wagner, M.; Leburton, J. P.

    1986-01-01

    A novel class of cascade structures is proposed which features multijunction upper subcells, referred to as superstructure high-efficiency photovoltaics (SHEPs). The additional junctions enhance spectral response and improve radiation tolerance by reducing bulk recombination losses. This is important because ternary III-V alloys, which tend to have short minority-carrier diffusion lengths, are the only viable materials for the high-bandgap upper subcells required for cascade solar cells. Realistic simulations of AlGaAs SHEPs show that one-sun AM0 efficiencies in excess of 26 percent are possible.

  11. Advanced high efficiency wraparound contact solar cell

    NASA Technical Reports Server (NTRS)

    Scott-Monck, J. A.; Uno, F. M.; Thornhill, J. W.

    1977-01-01

    A significant advancement in the development of thin high efficiency wraparound contact silicon solar cells has been made by coupling space and terrestrial processing procedures. Although this new method for fabricating cells has not been completely reduced to practice, some of the initial cells have delivered over 20 mW/sq cm when tested at 25 C under AMO intensity. This approach not only yields high efficiency devices, but shows promise of allowing complete freedom of choice in both the location and size of the wraparound contact pad area

  12. Advanced high efficiency wraparound contact solar cell

    NASA Technical Reports Server (NTRS)

    Scott-Monck, J. A.; Uno, F. M.; Thornhill, J. W.

    1977-01-01

    A significant advancement in the development of thin high efficiency wraparound contact silicon solar cells has been made by coupling space and terrestrial processing procedures. Although this new method for fabricating cells has not been completely reduced to practice, some of the initial cells have delivered over 20 mW/sq cm when tested at 25 C under AMO intensity. This approach not only yields high efficiency devices, but shows promise of allowing complete freedom of choice in both the location and size of the wraparound contact pad area.

  13. Highly efficient white OLEDs for lighting applications

    NASA Astrophysics Data System (ADS)

    Murano, Sven; Burghart, Markus; Birnstock, Jan; Wellmann, Philipp; Vehse, Martin; Werner, Ansgar; Canzler, Tobias; Stübinger, Thomas; He, Gufeng; Pfeiffer, Martin; Boerner, Herbert

    2005-10-01

    The use of organic light-emitting diodes (OLEDs) for large area general lighting purposes is gaining increasing interest during the recent years. Especially small molecule based OLEDs have already shown their potential for future applications. For white light emission OLEDs, power efficiencies exceeding that of incandescent bulbs could already be demonstrated, however additional improvements are needed to further mature the technology allowing for commercial applications as general purpose illuminating sources. Ultimately the efficiencies of fluorescent tubes should be reached or even excelled, a goal which could already be achieved in the past for green OLEDs.1 In this publication the authors will present highly efficient white OLEDs based on an intentional doping of the charge carrier transport layers and the usage of different state of the art emission principles. This presentation will compare white PIN-OLEDs based on phosphorescent emitters, fluorescent emitters and stacked OLEDs. It will be demonstrated that the reduction of the operating voltage by the use of intentionally doped transport layers leads to very high power efficiencies for white OLEDs, demonstrating power efficiencies of well above 20 lm/W @ 1000 cd/m2. The color rendering properties of the emitted light is very high and CRIs between 85 and 95 are achieved, therefore the requirements for standard applications in the field of lighting applications could be clearly fulfilled. The color coordinates of the light emission can be tuned within a wide range through the implementation of minor structural changes.

  14. High efficiency, variable geometry, centrifugal cryogenic pump

    SciTech Connect

    Forsha, M.D.; Nichols, K.E.; Beale, C.A.

    1994-12-31

    A centrifugal cryogenic pump has been developed which has a basic design that is rugged and reliable with variable speed and variable geometry features that achieve high pump efficiency over a wide range of head-flow conditions. The pump uses a sealless design and rolling element bearings to achieve high reliability and the ruggedness to withstand liquid-vapor slugging. The pump can meet a wide range of variable head, off-design flow requirements and maintain design point efficiency by adjusting the pump speed. The pump also has features that allow the impeller and diffuser blade heights to be adjusted. The adjustable height blades were intended to enhance the pump efficiency when it is operating at constant head, off-design flow rates. For small pumps, the adjustable height blades are not recommended. For larger pumps, they could provide off-design efficiency improvements. This pump was developed for supercritical helium service, but the design is well suited to any cryogenic application where high efficiency is required over a wide range of head-flow conditions.

  15. High-efficiency 20 W yellow VECSEL.

    PubMed

    Kantola, Emmi; Leinonen, Tomi; Ranta, Sanna; Tavast, Miki; Guina, Mircea

    2014-03-24

    A high-efficiency optically pumped vertical-external-cavity surface-emitting laser emitting 20 W at a wavelength around 588 nm is demonstrated. The semiconductor gain chip emitted at a fundamental wavelength around 1170-1180 nm and the laser employed a V-shaped cavity. The yellow spectral range was achieved by intra-cavity frequency doubling using a LBO crystal. The laser could be tuned over a bandwidth of ~26 nm while exhibiting watt-level output powers. The maximum conversion efficiency from absorbed pump power to yellow output was 28% for continuous wave operation. The VECSEL's output could be modulated to generate optical pulses with duration down to 570 ns by directly modulating the pump laser. The high-power pulse operation is a key feature for astrophysics and medical applications while at the same time enables higher slope efficiency than continuous wave operation owing to decreased heating.

  16. Requirements for high-efficiency solar cells

    NASA Technical Reports Server (NTRS)

    Sah, C. T.

    1986-01-01

    Minimum recombination and low injection level are essential for high efficiency. Twenty percent AM1 efficiency requires a dark recombination current density of 2 x 10 to the minus 13th power A/sq cm and a recombination center density of less than 10 to the 10th power /cu cm. Recombination mechanisms at thirteen locations in a conventional single crystalline silicon cell design are reviewed. Three additional recombination locations are described at grain boundaries in polycrystalline cells. Material perfection and fabrication process optimization requirements for high efficiency are outlined. Innovative device designs to reduce recombination in the bulk and interfaces of single crystalline cells and in the grain boundary of polycrystalline cells are reviewed.

  17. High efficiency novel window air conditioner

    DOE PAGES

    Bansal, Pradeep

    2015-01-01

    This paper presents the technical development of a high efficiency window air conditioner. In order to achieve higher energy efficiency ratio (EER), the original capacity of the R410A unit was downgraded by replacing the original compressor with a lower capacity but higher EER compressor, while all heat exchangers and the chassis from the original unit were retained. The other subsequent major modifications included – the AC fan motor being replaced with a brushless high efficiency electronically commuted motor (ECM) motor, the capillary tube being replaced with a needle valve to better control the refrigerant flow and refrigerant set points, andmore » R410A being replaced with drop-in environmentally friendly binary mixture of R32 (85% molar concentration)/R125 (15% molar concentration). All these modifications resulted in significant EER enhancement of the modified unit.« less

  18. High efficiency novel window air conditioner

    SciTech Connect

    Bansal, Pradeep

    2015-01-01

    This paper presents the technical development of a high efficiency window air conditioner. In order to achieve higher energy efficiency ratio (EER), the original capacity of the R410A unit was downgraded by replacing the original compressor with a lower capacity but higher EER compressor, while all heat exchangers and the chassis from the original unit were retained. The other subsequent major modifications included – the AC fan motor being replaced with a brushless high efficiency electronically commuted motor (ECM) motor, the capillary tube being replaced with a needle valve to better control the refrigerant flow and refrigerant set points, and R410A being replaced with drop-in environmentally friendly binary mixture of R32 (85% molar concentration)/R125 (15% molar concentration). All these modifications resulted in significant EER enhancement of the modified unit.

  19. Novel Nanophosphors for High Efficiency Fluorescent Lamps

    SciTech Connect

    Alok M. Srivastava

    2005-09-30

    This is the Yearly Report of the Novel Nanophosphors for High Efficiency Fluorescent Lamps, Department of Energy (DOE). The overall goal of this three-year program is to develop novel hybrid phosphors by coating commercially available lamp phosphors with highly stable wide band-gap nanocrystalline phosphors (NCP). The novel hybrid phosphors will increase the efficiency of the fluorescent lamps by up to 32%, enabling total energy savings of 0.26 quads, the reduction in the U.S. energy bill by $6.5 billion and the reduction of the annual carbon emission by 4.1 billion kilogram. The prime technical approach is the development of NCP quantum-splitting phosphor (QSP) and ultra-violet emitting phosphors with quantum efficiencies exceeding that of the conventional phosphors at 185 nm. Our chief achievement, during the current contract period, pertains to the successful synthesis and characterization of coated phosphors. We demonstrated several synthesis techniques for the coating of micron sized commercial phosphors with quantum-splitting and UV emitting nanophosphors. We have also continued our fundamental investigations into the physical processes that determine the quantum efficiency of the nanophosphors and this has further helped codify a set of rules for the host lattice that support efficient quantum splitting and UV emission at room temperature. In this report we summarize the technical work completed under the Program, summarize our findings about the performance limits of the various technologies we investigated, and outline promising paths for future work.

  20. High Efficiency Solar Integrated Roof Membrane Product

    SciTech Connect

    Partyka, Eric; Shenoy, Anil

    2013-05-15

    This project was designed to address the Solar Energy Technology Program objective, to develop new methods to integrate photovoltaic (PV) cells or modules within a building-integrated photovoltaic (BIPV) application that will result in lower installed cost as well as higher efficiencies of the encapsulated/embedded PV module. The technology assessment and development focused on the evaluation and identification of manufacturing technologies and equipment capable of producing such low-cost, high-efficiency, flexible BIPV solar cells on single-ply roofing membranes.

  1. Transduction efficiency of neurons and glial cells by AAV-1, -5, -9, -rh10 and -hu11 serotypes in rat spinal cord following contusion injury.

    PubMed

    Petrosyan, H A; Alessi, V; Singh, V; Hunanyan, A S; Levine, J M; Arvanian, V L

    2014-12-01

    Adeno-associated viruses (AAVs) are a promising system for therapeutic gene delivery to neurons in a number of neurodegenerative conditions including spinal cord injuries (SCIs). Considering the role of macrophages and glia in the progression of 'secondary damage', we searched for the optimal vectors for gene transfer to both neurons and glia following contusion SCI in adult rats. Contusion models share many similarities to most human spinal cord traumas. Several AAV serotypes known for their neuronal tropism expressing enhanced green-fluorescent protein (GFP) were injected intraspinally following thoracic T10 contusion. We systematically compared the transduction efficacy and cellular tropism of these vectors for neurons, macrophages/microglia, oligodendrocytes, astrocytes and NG2-positive glial cells following contusion SCI. No additional changes in inflammatory responses or behavioral performance were observed for any of the vectors. We identified that AAV-rh10 induced robust transduction of both neuronal and glial cells. Even though efficacy to transduce neurons was comparable to already established AAV-1, AAV-5 and AAV-9, AAV-rh10 transduced significantly higher number of macrophages/microglia and oligodendrocytes in damaged spinal cord compared with other serotypes tested. Thus, AAV-rh10 carries promising potential as a gene therapy vector, particularly if both the neuronal and glial cell populations in damaged spinal cord are targeted.

  2. Highly efficient solid state magnetoelectric gyrators

    NASA Astrophysics Data System (ADS)

    Leung, Chung Ming; Zhuang, Xin; Friedrichs, Daniel; Li, Jiefang; Erickson, Robert W.; Laletin, V.; Popov, M.; Srinivasan, G.; Viehland, D.

    2017-09-01

    An enhancement in the power-conversion-efficiency (η) of a magneto-electric (ME) gyrator has been found by the use of Mn-substituted nickel zinc ferrite. A trilayer gyrator of Mn-doped Ni0.8Zn0.2Fe2O3 and Pb(Zr,Ti)O3 has η = 85% at low power conditions (˜20 mW/in3) and η ≥ 80% at high power conditions (˜5 W/in3). It works close to fundamental electromechanical resonance in both direct and converse modes. The value of η is by far the highest reported so far, which is due to the high mechanical quality factor (Qm) of the magnetostrictive ferrite. Such highly efficient ME gyrators with a significant power density could become important elements in power electronics, potentially replacing electromagnetic and piezoelectric transformers.

  3. Efficient circuit triggers high-current, high-voltage pulses

    NASA Technical Reports Server (NTRS)

    Green, E. D.

    1964-01-01

    Modified circuit uses diodes to effectively disconnect the charging resistors from the circuit during the discharge cycle. Result is an efficient parallel charging, high voltage pulse modulator with low voltage rating of components.

  4. High-Temperature High-Efficiency Solar Thermoelectric Generators

    NASA Astrophysics Data System (ADS)

    Baranowski, Lauryn L.; Warren, Emily L.; Toberer, Eric S.

    2014-06-01

    Inspired by recent high-efficiency thermoelectric modules, we consider thermoelectrics for terrestrial applications in concentrated solar thermoelectric generators (STEGs). The STEG is modeled as two subsystems: a TEG, and a solar absorber that efficiently captures the concentrated sunlight and limits radiative losses from the system. The TEG subsystem is modeled using thermoelectric compatibility theory; this model does not constrain the material properties to be constant with temperature. Considering a three-stage TEG based on current record modules, this model suggests that 18% efficiency could be experimentally expected with a temperature gradient of 1000°C to 100°C. Achieving 15% overall STEG efficiency thus requires an absorber efficiency above 85%, and we consider two methods to achieve this: solar-selective absorbers and thermally insulating cavities. When the TEG and absorber subsystem models are combined, we expect that the STEG modeled here could achieve 15% efficiency with optical concentration between 250 and 300 suns.

  5. High-Temperature High-Efficiency Solar Thermoelectric Generators

    SciTech Connect

    Baranowski, LL; Warren, EL; Toberer, ES

    2014-03-01

    Inspired by recent high-efficiency thermoelectric modules, we consider thermoelectrics for terrestrial applications in concentrated solar thermoelectric generators (STEGs). The STEG is modeled as two subsystems: a TEG, and a solar absorber that efficiently captures the concentrated sunlight and limits radiative losses from the system. The TEG subsystem is modeled using thermoelectric compatibility theory; this model does not constrain the material properties to be constant with temperature. Considering a three-stage TEG based on current record modules, this model suggests that 18% efficiency could be experimentally expected with a temperature gradient of 1000A degrees C to 100A degrees C. Achieving 15% overall STEG efficiency thus requires an absorber efficiency above 85%, and we consider two methods to achieve this: solar-selective absorbers and thermally insulating cavities. When the TEG and absorber subsystem models are combined, we expect that the STEG modeled here could achieve 15% efficiency with optical concentration between 250 and 300 suns.

  6. High-efficiency silicon concentrator cell commercialization

    SciTech Connect

    Sinton, R.A.; Swanson, R.M.

    1993-05-01

    This report summarizes the first phase of a forty-one month program to develop a commercial, high-efficiency concentrator solar cell and facility for manufacturing it. The period covered is November 1, 1990 to December 31, 1991. This is a joint program between the Electric Power Research Institute (EPRI) and Sandia National Laboratories. (This report is also published by EPRI as EPRI report number TR-102035.) During the first year of the program, SunPower accomplished the following major objectives: (1) a new solar cell fabrication facility, which is called the Cell Pilot Line (CPL), (2) a baseline concentrator cell process has been developed, and (3) a cell testing facility has been completed. Initial cell efficiencies are about 23% for the baseline process. The long-range goal is to improve this efficiency to 27%.

  7. Methodologies for high efficiency perovskite solar cells.

    PubMed

    Park, Nam-Gyu

    2016-01-01

    Since the report on long-term durable solid-state perovskite solar cell in 2012, perovskite solar cells based on lead halide perovskites having organic cations such as methylammonium CH3NH3PbI3 or formamidinium HC(NH2)2PbI3 have received great attention because of superb photovoltaic performance with power conversion efficiency exceeding 22 %. In this review, emergence of perovskite solar cell is briefly introduced. Since understanding fundamentals of light absorbers is directly related to their photovoltaic performance, opto-electronic properties of organo lead halide perovskites are investigated in order to provide insight into design of higher efficiency perovskite solar cells. Since the conversion efficiency of perovskite solar cell is found to depend significantly on perovskite film quality, methodologies for fabricating high quality perovskite films are particularly emphasized, including various solution-processes and vacuum deposition method.

  8. Methodologies for high efficiency perovskite solar cells

    NASA Astrophysics Data System (ADS)

    Park, Nam-Gyu

    2016-06-01

    Since the report on long-term durable solid-state perovskite solar cell in 2012, perovskite solar cells based on lead halide perovskites having organic cations such as methylammonium CH3NH3PbI3 or formamidinium HC(NH2)2PbI3 have received great attention because of superb photovoltaic performance with power conversion efficiency exceeding 22 %. In this review, emergence of perovskite solar cell is briefly introduced. Since understanding fundamentals of light absorbers is directly related to their photovoltaic performance, opto-electronic properties of organo lead halide perovskites are investigated in order to provide insight into design of higher efficiency perovskite solar cells. Since the conversion efficiency of perovskite solar cell is found to depend significantly on perovskite film quality, methodologies for fabricating high quality perovskite films are particularly emphasized, including various solution-processes and vacuum deposition method.

  9. High efficiency electrotransformation of Lactobacillus casei.

    PubMed

    Welker, Dennis L; Hughes, Joanne E; Steele, James L; Broadbent, Jeff R

    2015-01-01

    We investigated whether protocols allowing high efficiency electrotransformation of other lactic acid bacteria were applicable to five strains of Lactobacillus casei (12A, 32G, A2-362, ATCC 334 and BL23). Addition of 1% glycine or 0.9 M NaCl during cell growth, limitation of the growth of the cell cultures to OD600 0.6-0.8, pre-electroporation treatment of cells with water or with a lithium acetate (100 mM)/dithiothreitol (10 mM) solution and optimization of electroporation conditions all improved transformation efficiencies. However, the five strains varied in their responses to these treatments. Transformation efficiencies of 10(6) colony forming units μg(-1) pTRKH2 DNA and higher were obtained with three strains which is sufficient for construction of chromosomal gene knock-outs and gene replacements.

  10. High Efficiency, Low Emission Refrigeration System

    SciTech Connect

    Fricke, Brian A.; Sharma, Vishaldeep

    2016-08-01

    Supermarket refrigeration systems account for approximately 50% of supermarket energy use, placing this class of equipment among the highest energy consumers in the commercial building domain. In addition, the commonly used refrigeration system in supermarket applications is the multiplex direct expansion (DX) system, which is prone to refrigerant leaks due to its long lengths of refrigerant piping. This leakage reduces the efficiency of the system and increases the impact of the system on the environment. The high Global Warming Potential (GWP) of the hydrofluorocarbon (HFC) refrigerants commonly used in these systems, coupled with the large refrigerant charge and the high refrigerant leakage rates leads to significant direct emissions of greenhouse gases into the atmosphere. Methods for reducing refrigerant leakage and energy consumption are available, but underutilized. Further work needs to be done to reduce costs of advanced system designs to improve market utilization. In addition, refrigeration system retrofits that result in reduced energy consumption are needed since the majority of applications address retrofits rather than new stores. The retrofit market is also of most concern since it involves large-volume refrigerant systems with high leak rates. Finally, alternative refrigerants for new and retrofit applications are needed to reduce emissions and reduce the impact on the environment. The objective of this Collaborative Research and Development Agreement (CRADA) between the Oak Ridge National Laboratory and Hill Phoenix is to develop a supermarket refrigeration system that reduces greenhouse gas emissions and has 25 to 30 percent lower energy consumption than existing systems. The outcomes of this project will include the design of a low emission, high efficiency commercial refrigeration system suitable for use in current U.S. supermarkets. In addition, a prototype low emission, high efficiency supermarket refrigeration system will be produced for

  11. High-speed high-efficiency photodetectors based on heterostructures

    NASA Astrophysics Data System (ADS)

    Korolkov, V. I.

    Recent advances in the development of high-speed high-efficiency heterostructure photodetectors (HPs) are reviewed. It is noted that the performance of semiconductor photodetectors has been improved by forbidden bandwidth control. Various types of HPs are examined, including modifications of heterophotodiodes and detectors with internal amplification; avalanche photodiodes; bipolar phototransistors; and planar photoresistance devices and field-effect phototransistors. These devices are compared in terms of speed and efficiency.

  12. Transductive face sketch-photo synthesis.

    PubMed

    Wang, Nannan; Tao, Dacheng; Gao, Xinbo; Li, Xuelong; Li, Jie

    2013-09-01

    Face sketch-photo synthesis plays a critical role in many applications, such as law enforcement and digital entertainment. Recently, many face sketch-photo synthesis methods have been proposed under the framework of inductive learning, and these have obtained promising performance. However, these inductive learning-based face sketch-photo synthesis methods may result in high losses for test samples, because inductive learning minimizes the empirical loss for training samples. This paper presents a novel transductive face sketch-photo synthesis method that incorporates the given test samples into the learning process and optimizes the performance on these test samples. In particular, it defines a probabilistic model to optimize both the reconstruction fidelity of the input photo (sketch) and the synthesis fidelity of the target output sketch (photo), and efficiently optimizes this probabilistic model by alternating optimization. The proposed transductive method significantly reduces the expected high loss and improves the synthesis performance for test samples. Experimental results on the Chinese University of Hong Kong face sketch data set demonstrate the effectiveness of the proposed method by comparing it with representative inductive learning-based face sketch-photo synthesis methods.

  13. Nanopatterned Quantum Dot Lasers for High Speed, High Efficiency, Operation

    DTIC Science & Technology

    2015-04-27

    SECURITY CLASSIFICATION OF: Quantum dot (QD) active regions hold potential for realizing extremely high performance semiconductor diode lasers...2009 31-Dec-2014 Approved for Public Release; Distribution Unlimited Final Report: Nanopatterned Quantum Dot Lasers for High Speed, High Efficiency...Research Office P.O. Box 12211 Research Triangle Park, NC 27709-2211 quantum dots , nanopatterning, MOCVD, laser REPORT DOCUMENTATION PAGE 11

  14. Breeding for high water-use efficiency.

    PubMed

    Condon, A G; Richards, R A; Rebetzke, G J; Farquhar, G D

    2004-11-01

    There is a pressing need to improve the water-use efficiency of rain-fed and irrigated crop production. Breeding crop varieties with higher water-use efficiency is seen as providing part of the solution. Three key processes can be exploited in breeding for high water-use efficiency: (i) moving more of the available water through the crop rather than it being wasted as evaporation from the soil surface or drainage beyond the root zone or being left behind in the root zone at harvest; (ii) acquiring more carbon (biomass) in exchange for the water transpired by the crop, i.e. improving crop transpiration efficiency; (iii) partitioning more of the achieved biomass into the harvested product. The relative importance of any one of these processes will vary depending on how water availability varies during the crop cycle. However, these three processes are not independent. Targeting specific traits to improve one process may have detrimental effects on the other two, but there may also be positive interactions. Progress in breeding for improved water-use efficiency of rain-fed wheat is reviewed to illustrate the nature of some of these interactions and to highlight opportunities that may be exploited in other crops as well as potential pitfalls. For C3 species, measuring carbon isotope discrimination provides a powerful means of improving water-use efficiency of leaf gas exchange, but experience has shown that improvements in leaf-level water-use efficiency may not always translate into higher crop water-use efficiency or yield. In fact, the reverse has frequently been observed. Reasons for this are explored in some detail. Crop simulation modelling can be used to assess the likely impact on water-use efficiency and yield of changing the expression of traits of interest. Results of such simulations indicate that greater progress may be achieved by pyramiding traits so that potential negative effects of individual traits are neutralized. DNA-based selection techniques may

  15. Creation of High Efficient Firefly Luciferase

    NASA Astrophysics Data System (ADS)

    Nakatsu, Toru

    Firefly emits visible yellow-green light. The bioluminescence reaction is carried out by the enzyme luciferase. The bioluminescence of luciferase is widely used as an excellent tool for monitoring gene expression, the measurement of the amount of ATP and in vivo imaging. Recently a study of the cancer metastasis is carried out by in vivo luminescence imaging system, because luminescence imaging is less toxic and more useful for long-term assay than fluorescence imaging by GFP. However the luminescence is much dimmer than fluorescence. Then bioluminescence imaging in living organisms demands the high efficient luciferase which emits near infrared lights or enhances the emission intensity. Here I introduce an idea for creating the high efficient luciferase based on the crystal structure.

  16. Efficient High-Pressure State Equations

    NASA Technical Reports Server (NTRS)

    Harstad, Kenneth G.; Miller, Richard S.; Bellan, Josette

    1997-01-01

    A method is presented for a relatively accurate, noniterative, computationally efficient calculation of high-pressure fluid-mixture equations of state, especially targeted to gas turbines and rocket engines. Pressures above I bar and temperatures above 100 K are addressed The method is based on curve fitting an effective reference state relative to departure functions formed using the Peng-Robinson cubic state equation Fit parameters for H2, O2, N2, propane, methane, n-heptane, and methanol are given.

  17. High-efficiency silicon solar cells

    NASA Technical Reports Server (NTRS)

    Olsen, L. C.

    1985-01-01

    Fabrication and characterization of high-efficiency metal insulator, n-p (MINP) cells is described. Particular attention was paid to development of measurement methods for surface recombination and density of surface states. A modified Rosier test structure was used successfully for density of surface states. Silicon oxide and silicon nitride passivants were studied. Heat treatment after plasma enhanced chemical vapor deposition (CVD) of silicon nitride was shown to be beneficial. A more optimum emitter concentration profile was modeled.

  18. High energy efficient solid state laser sources

    NASA Technical Reports Server (NTRS)

    Byer, Robert L.

    1987-01-01

    Diode-laser-pumped solid-state laser oscillators and nonlinear processes were investigated. A new generation on nonplanar oscillator was fabricated, and it is anticipated that passive linewidths will be pushed to the kilohertz regime. A number of diode-pumped laser transitions were demonstrated in the rod configuration. Second-harmonic conversion efficiencies as high as 15% are routinely obtained in a servo-locked external resonant doubling crystal at 15 mW cw input power levels at 1064 nm.

  19. High Efficiency Thermoelectric Materials and Devices

    NASA Technical Reports Server (NTRS)

    Kochergin, Vladimir (Inventor)

    2013-01-01

    Growth of thermoelectric materials in the form of quantum well super-lattices on three-dimensionally structured substrates provide the means to achieve high conversion efficiency of the thermoelectric module combined with inexpensiveness of fabrication and compatibility with large scale production. Thermoelectric devices utilizing thermoelectric materials in the form of quantum well semiconductor super-lattices grown on three-dimensionally structured substrates provide improved thermoelectric characteristics that can be used for power generation, cooling and other applications..

  20. High efficiency, long-life photocathodes

    NASA Astrophysics Data System (ADS)

    Ives, Lawrence; Montgomery, Eric; Jensen, Kevin; Collins, George; Marsden, David; Karimov, Rasul; Falce, Lou

    2017-03-01

    Research and development on high efficiency, robust, long-life photocathodes is in progress for accelerator, light source, and other commercial applications. The research is investigating detailed physics of photoemission and developing a computational capability to predict performance. Reservoir technology will significantly increase lifetime and allow recovery from many poisoning events. Better understanding of the physics will impact fabrication techniques to optimize performance. A production facility is under construction to provide improved photocathodes to users.

  1. Efficient High-Pressure State Equations

    NASA Technical Reports Server (NTRS)

    Harstad, Kenneth G.; Miller, Richard S.; Bellan, Josette

    1997-01-01

    A method is presented for a relatively accurate, noniterative, computationally efficient calculation of high-pressure fluid-mixture equations of state, especially targeted to gas turbines and rocket engines. Pressures above I bar and temperatures above 100 K are addressed The method is based on curve fitting an effective reference state relative to departure functions formed using the Peng-Robinson cubic state equation Fit parameters for H2, O2, N2, propane, methane, n-heptane, and methanol are given.

  2. Bioblendstocks that Enable High Efficiency Engine Designs

    SciTech Connect

    McCormick, Robert L.; Fioroni, Gina M.; Ratcliff, Matthew A.; Zigler, Bradley T.; Farrell, John

    2016-11-03

    The past decade has seen a high level of innovation in production of biofuels from sugar, lipid, and lignocellulose feedstocks. As discussed in several talks at this workshop, ethanol blends in the E25 to E50 range could enable more highly efficient spark-ignited (SI) engines. This is because of their knock resistance properties that include not only high research octane number (RON), but also charge cooling from high heat of vaporization, and high flame speed. Emerging alcohol fuels such as isobutanol or mixed alcohols have desirable properties such as reduced gasoline blend vapor pressure, but also have lower RON than ethanol. These fuels may be able to achieve the same knock resistance benefits, but likely will require higher blend levels or higher RON hydrocarbon blendstocks. A group of very high RON (>150) oxygenates such as dimethyl furan, methyl anisole, and related compounds are also produced from biomass. While providing no increase in charge cooling, their very high octane numbers may provide adequate knock resistance for future highly efficient SI engines. Given this range of options for highly knock resistant fuels there appears to be a critical need for a fuel knock resistance metric that includes effects of octane number, heat of vaporization, and potentially flame speed. Emerging diesel fuels include highly branched long-chain alkanes from hydroprocessing of fats and oils, as well as sugar-derived terpenoids. These have relatively high cetane number (CN), which may have some benefits in designing more efficient CI engines. Fast pyrolysis of biomass can produce diesel boiling range streams that are high in aromatic, oxygen and acid contents. Hydroprocessing can be applied to remove oxygen and consequently reduce acidity, however there are strong economic incentives to leave up to 2 wt% oxygen in the product. This oxygen will primarily be present as low CN alkyl phenols and aryl ethers. While these have high heating value, their presence in diesel fuel

  3. Evaluating performance of high efficiency mist eliminators

    SciTech Connect

    Waggoner, Charles A.; Parsons, Michael S.; Giffin, Paxton K.

    2013-07-01

    Processing liquid wastes frequently generates off gas streams with high humidity and liquid aerosols. Droplet laden air streams can be produced from tank mixing or sparging and processes such as reforming or evaporative volume reduction. Unfortunately these wet air streams represent a genuine threat to HEPA filters. High efficiency mist eliminators (HEME) are one option for removal of liquid aerosols with high dissolved or suspended solids content. HEMEs have been used extensively in industrial applications, however they have not seen widespread use in the nuclear industry. Filtering efficiency data along with loading curves are not readily available for these units and data that exist are not easily translated to operational parameters in liquid waste treatment plants. A specialized test stand has been developed to evaluate the performance of HEME elements under use conditions of a US DOE facility. HEME elements were tested at three volumetric flow rates using aerosols produced from an iron-rich waste surrogate. The challenge aerosol included submicron particles produced from Laskin nozzles and super micron particles produced from a hollow cone spray nozzle. Test conditions included ambient temperature and relative humidities greater than 95%. Data collected during testing HEME elements from three different manufacturers included volumetric flow rate, differential temperature across the filter housing, downstream relative humidity, and differential pressure (dP) across the filter element. Filter challenge was discontinued at three intermediate dPs and the filter to allow determining filter efficiency using dioctyl phthalate and then with dry surrogate aerosols. Filtering efficiencies of the clean HEME, the clean HEME loaded with water, and the HEME at maximum dP were also collected using the two test aerosols. Results of the testing included differential pressure vs. time loading curves for the nine elements tested along with the mass of moisture and solid

  4. High efficiency crystalline silicon solar cells

    NASA Technical Reports Server (NTRS)

    Sah, C. Tang

    1986-01-01

    A review of the entire research program since its inception ten years ago is given. The initial effort focused on the effects of impurities on the efficiency of silicon solar cells to provide figures of maximum allowable impurity density for efficiencies up to about 16 to 17%. Highly accurate experimental techniques were extended to characterize the recombination properties of the residual imputities in the silicon solar cell. A numerical simulator of the solar cell was also developed, using the Circuit Technique for Semiconductor Analysis. Recent effort focused on the delineation of the material and device parameters which limited the silicon efficiency to below 20% and on an investigation of cell designs to break the 20% barrier. Designs of the cell device structure and geometry can further reduce recombination losses as well as the sensitivity and criticalness of the fabrication technology required to exceed 20%. Further research is needed on the fundamental characterization of the carrier recombination properties at the chemical impurity and physical defect centers. It is shown that only single crystalline silicon cell technology can be successful in attaining efficiencies greater than 20%.

  5. High-efficiency organic electrophosphorescent devices

    NASA Astrophysics Data System (ADS)

    Thompson, Mark E.; Lamansky, Sergey; Djurovich, Peter; Murphy, Drew; Abdel-Razaq, Feras; Forrest, Stephen R.; Baldo, Marc A.; Burrows, Paul E.; Adachi, Chihaya; Zhou, Theodore X.; Michalski, Lech A.; Rajan, Kamala; Brown, Julie J.

    2001-02-01

    We have fabricated saturated red, orange, yellow and green OLEDs, utilizing phosphorescent dopants. Using phosphorescence based emitters we have eliminated the inherent 25% upper limit on emission observed for traditional fluorescence based systems. The quantum efficiencies of these devices are quite good, with measured external efficiencies > 15% and > 40 lum/W (green) in the best devices. The phosphorescent dopants in these devices are heavy metal containing molecules (i.e. Pt, and Ir), prepared as both metalloporphyrins and organometallic complexes. The high level of spin orbit coupling in these metal complexes gives efficient emission from triplet states. In addition to emission from the heavy metal dopant, it is possible to transfer the exciton energy to a fluorescent dye, by Forster energy transfer. The heavy metal dopant in this case acts as a sensitizer, utilizing both singlet and triplet excitons to efficiently pump a fluorescent dye. We discuss the important parameters in designing electrophosphorescent OLEDs as well as their strengths and limitations. Accelerated aging studies, on packaged devices, have shown that phosphorescence based OLEDs can have very long device lifetimes.

  6. Highly efficient fully transparent inverted OLEDs

    NASA Astrophysics Data System (ADS)

    Meyer, J.; Winkler, T.; Hamwi, S.; Schmale, S.; Kröger, M.; Görrn, P.; Johannes, H.-H.; Riedl, T.; Lang, E.; Becker, D.; Dobbertin, T.; Kowalsky, W.

    2007-09-01

    One of the unique selling propositions of OLEDs is their potential to realize highly transparent devices over the visible spectrum. This is because organic semiconductors provide a large Stokes-Shift and low intrinsic absorption losses. Hence, new areas of applications for displays and ambient lighting become accessible, for instance, the integration of OLEDs into the windshield or the ceiling of automobiles. The main challenge in the realization of fully transparent devices is the deposition of the top electrode. ITO is commonly used as transparent bottom anode in a conventional OLED. To obtain uniform light emission over the entire viewing angle and a low series resistance, a TCO such as ITO is desirable as top contact as well. However, sputter deposition of ITO on top of organic layers causes damage induced by high energetic particles and UV radiation. We have found an efficient process to protect the organic layers against the ITO rf magnetron deposition process of ITO for an inverted OLED (IOLED). The inverted structure allows the integration of OLEDs in more powerful n-channel transistors used in active matrix backplanes. Employing the green electrophosphorescent material Ir(ppy) 3 lead to IOLED with a current efficiency of 50 cd/A and power efficiency of 24 lm/W at 100 cd/m2. The average transmittance exceeds 80 % in the visible region. The on-set voltage for light emission is lower than 3 V. In addition, by vertical stacking we achieved a very high current efficiency of more than 70 cd/A for transparent IOLED.

  7. Effects of Adenoviral Gene Transduction on the Stemness of Human Bone Marrow Mesenchymal Stem Cells

    PubMed Central

    Marasini, Subash; Chang, Da-Young; Jung, Jin-Hwa; Lee, Su-Jung; Cha, Hye Lim; Suh-Kim, Haeyoung; Kim, Sung-Soo

    2017-01-01

    Human mesenchymal stem cells (MSCs) are currently being evaluated as a cell-based therapy for tissue injury and degenerative diseases. Recently, several methods have been suggested to further enhance the therapeutic functions of MSCs, including genetic modifications with tissue- and/or disease-specific genes. The objective of this study was to examine the efficiency and stability of transduction using an adenoviral vector in human MSCs. Additionally, we aimed to assess the effects of transduction on the proliferation and multipotency of MSCs. The results indicate that MSCs can be transduced by adenoviruses in vitro, but high viral titers are necessary to achieve high efficiency. In addition, transduction at a higher multiplicity of infection (MOI) was associated with attenuated proliferation and senescence-like morphology. Furthermore, transduced MSCs showed a diminished capacity for adipogenic differentiation while retaining their potential to differentiate into osteocytes and chondrocytes. This work could contribute significantly to clinical trials of MSCs modified with therapeutic genes. PMID:28835020

  8. High Efficiency Colloidal Quantum Dot Phosphors

    SciTech Connect

    Kahen, Keith

    2013-12-31

    The project showed that non-Cd containing, InP-based nanocrystals (semiconductor materials with dimensions of ~6 nm) have high potential for enabling next-generation, nanocrystal-based, on chip phosphors for solid state lighting. Typical nanocrystals fall short of the requirements for on chip phosphors due to their loss of quantum efficiency under the operating conditions of LEDs, such as, high temperature (up to 150 °C) and high optical flux (up to 200 W/cm2). The InP-based nanocrystals invented during this project maintain high quantum efficiency (>80%) in polymer-based films under these operating conditions for emission wavelengths ranging from ~530 to 620 nm. These nanocrystals also show other desirable attributes, such as, lack of blinking (a common problem with nanocrystals which limits their performance) and no increase in the emission spectral width from room to 150 °C (emitters with narrower spectral widths enable higher efficiency LEDs). Prior to these nanocrystals, no nanocrystal system (regardless of nanocrystal type) showed this collection of properties; in fact, other nanocrystal systems are typically limited to showing only one desirable trait (such as high temperature stability) but being deficient in other properties (such as high flux stability). The project showed that one can reproducibly obtain these properties by generating a novel compositional structure inside of the nanomaterials; in addition, the project formulated an initial theoretical framework linking the compositional structure to the list of high performance optical properties. Over the course of the project, the synthetic methodology for producing the novel composition was evolved to enable the synthesis of these nanomaterials at a cost approximately equal to that required for forming typical conventional nanocrystals. Given the above results, the last major remaining step prior to scale up of the nanomaterials is to limit the oxidation of these materials during the tens of

  9. Practical high efficiency bifacial solar cells

    SciTech Connect

    Moehlecke, A.; Zanesco, I.; Luque, A.

    1994-12-31

    In this paper, the authors present a practical process to obtain bifacial solar cells. These cells are made using p{sup +}nn{sup +} structure on high-medium base resistivity, continuous emitters and with a process that maintains high bulk minority carrier lifetime. Efficiencies of 19.1% and 18.1% are achieved under standard conditions when the cell is illuminated by n{sup +}n high-low junction and when it is illuminated by P{sup +}n junction, respectively. The authors remark that the n{sup +}n high-low junction provides a higher current density and a good ratio between generated current of each face is found to be of about 103%.

  10. New high-efficiency silicon solar cells

    NASA Technical Reports Server (NTRS)

    Daud, T.; Crotty, G. T.

    1985-01-01

    A design for silicon solar cells was investigated as an approach to increasing the cell open-circuit voltage and efficiency for flat-plate terrestrial photovoltaic applications. This deviates from past designs, where either the entire front surface of the cell is covered by a planar junction or the surface is textured before junction formation, which results in an even greater (up to 70%) junction area. The heavily doped front region and the junction space charge region are potential areas of high recombination for generated and injected minority carriers. The design presented reduces junction area by spreading equidiameter dot junctions across the surface of the cell, spaced about a diffusion length or less from each other. Various dot diameters and spacings allowed variations in total junction area. A simplified analysis was done to obtain a first-order design optimization. Efficiencies of up to 19% can be obtained. Cell fabrication involved extra masking steps for selective junction diffusion, and made surface passivation a key element in obtaining good collection. It also involved photolithography, with line widths down to microns. A method is demonstrated for achieving potentially high open-circuit voltages and solar-cell efficiencies.

  11. High-efficiency indoor air mover

    SciTech Connect

    Ariewitz, D.; Lackey, R.S.; Veyo, S.E.

    1983-01-06

    A high-efficiency indoor air mover has been developed for the advanced electric heat pump. Preprototype air mover overall efficiency is approximately 40 percent, twice that of the conventionally applied squirrel-cage blower. The air mover consists of a 411 mm (16.2 inch) diameter single entry blower wheel carried in a volute sheet steel scroll and driven by a 250 W (1/3 horsepower) high-efficiency motor. The blower wheel uses ten backward curved, uniformly spaced, cambered plate blades. As installed in the advanced heat pump the air mover consumes 390 W of electrical power and delivers 662 l/s (1405 scfm) at 1092 rpm into an external static flow resistance of 87 Pa (0.35 inches of water). Although this air mover will cost about twice as much as the conventional squirrel-cage blower and motor of comparable flow performance the incremental premium cost at the retail level can be recovered in less than one year through energy savings assuming 6000 hours of operation per year with electricity at $0.05/kWh.

  12. A high-efficiency indoor air mover

    SciTech Connect

    Ariewitz, D.; Lackey, R.S.; Veyo, S.E.

    1983-06-01

    A high-efficiency indoor air mover has been developed for the advanced electric heat pump. Preprototype air mover overall efficiency is approximately 46%, more than twice that of the conventionally applied squirrel-cage blower. The air mover consists of a 16.25 in (413 mm) diameter single-entry blower wheel carried in a volute sheet steel scroll and driven by a 1/3 hp (250 W) high-efficiency motor. The blower wheel uses ten backward curved, uniformly spaced, cambered plate blades. As installed in the advanced heat pump, the air mover consumes 390 W of electrical power and delivers 1405 scfm (663 L/s) at 1092 rpm into an estimated overall static-flow resistance of 1.09 in of water (271 Pa). Although this air mover will cost about twice as much as the conventional squirrel-cage blower and motor of comparable flow performance, the incremental premium cost at the retail level can be recovered in less than one year through energy savings with electricity at $0.05/kWh in Minneapolis, where the blower would run approximately 4900 hours per year.

  13. High-efficiency red electrophosphorescence devices

    NASA Astrophysics Data System (ADS)

    Adachi, Chihaya; Baldo, Marc A.; Forrest, Stephen R.; Lamansky, Sergey; Thompson, Mark E.; Kwong, Raymond C.

    2001-03-01

    We demonstrate high-efficiency red electrophosphorescent organic light-emitting devices employing bis(2-(2'-benzo[4,5-a]thienyl)pyridinato-N,C3') iridium(acetylacetonate) [Btp2Ir(acac)] as a red phosphor. A maximum external quantum efficiency of ηext=(7.0±0.5)% and power efficiency of ηp=(4.6±0.5) lm/W are achieved at a current density of J=0.01 mA/cm2. At a higher current density of J=100 mA/cm2, ηext=(2.5±0.3)% and ηp=(0.56±0.05) lm/W are obtained. The electroluminescent spectrum has a maximum at a wavelength of λmax=616 nm with additional intensity peaks at λsub=670 and 745 nm. The Commission Internationale de L'Eclairage coordinates of (x=0.68, y=0.32) are close to meeting video display standards. The short phosphorescence lifetime (˜4 μs) of Btp2Ir(acac) leads to a significant improvement in ηext at high currents as compared to the previously reported red phosphor, 2,3,7,8,12,13,17,18-octaethyl-12H, 23H-prophine platinum (II) PtOEP with a lifetime of ˜50 μs.

  14. High-efficiency Autonomous Laser Adaptive Optics

    NASA Astrophysics Data System (ADS)

    Baranec, Christoph; Riddle, Reed; Law, Nicholas M.; Ramaprakash, A. N.; Tendulkar, Shriharsh; Hogstrom, Kristina; Bui, Khanh; Burse, Mahesh; Chordia, Pravin; Das, Hillol; Dekany, Richard; Kulkarni, Shrinivas; Punnadi, Sujit

    2014-07-01

    As new large-scale astronomical surveys greatly increase the number of objects targeted and discoveries made, the requirement for efficient follow-up observations is crucial. Adaptive optics imaging, which compensates for the image-blurring effects of Earth's turbulent atmosphere, is essential for these surveys, but the scarcity, complexity and high demand of current systems limit their availability for following up large numbers of targets. To address this need, we have engineered and implemented Robo-AO, a fully autonomous laser adaptive optics and imaging system that routinely images over 200 objects per night with an acuity 10 times sharper at visible wavelengths than typically possible from the ground. By greatly improving the angular resolution, sensitivity, and efficiency of 1-3 m class telescopes, we have eliminated a major obstacle in the follow-up of the discoveries from current and future large astronomical surveys.

  15. HIGH-EFFICIENCY AUTONOMOUS LASER ADAPTIVE OPTICS

    SciTech Connect

    Baranec, Christoph; Riddle, Reed; Tendulkar, Shriharsh; Hogstrom, Kristina; Bui, Khanh; Dekany, Richard; Kulkarni, Shrinivas; Law, Nicholas M.; Ramaprakash, A. N.; Burse, Mahesh; Chordia, Pravin; Das, Hillol; Punnadi, Sujit

    2014-07-20

    As new large-scale astronomical surveys greatly increase the number of objects targeted and discoveries made, the requirement for efficient follow-up observations is crucial. Adaptive optics imaging, which compensates for the image-blurring effects of Earth's turbulent atmosphere, is essential for these surveys, but the scarcity, complexity and high demand of current systems limit their availability for following up large numbers of targets. To address this need, we have engineered and implemented Robo-AO, a fully autonomous laser adaptive optics and imaging system that routinely images over 200 objects per night with an acuity 10 times sharper at visible wavelengths than typically possible from the ground. By greatly improving the angular resolution, sensitivity, and efficiency of 1-3 m class telescopes, we have eliminated a major obstacle in the follow-up of the discoveries from current and future large astronomical surveys.

  16. Highly efficient Raman distributed feedback fibre lasers.

    PubMed

    Shi, Jindan; Alam, Shaif-ul; Ibsen, Morten

    2012-02-27

    We demonstrate highly efficient Raman distributed feedback (DFB) fibre lasers for the first time with up to 1.6 W of continuous wave (CW) output power. The DFB Bragg gratings are written directly into two types of commercially available passive germano-silica fibres. Two lasers of 30 cm length are pumped with up to 15 W of CW power at 1068 nm. The threshold power is ~2 W for a Raman-DFB (R-DFB) laser written in standard low-NA fibre, and only ~1 W for a laser written in a high-NA fibre, both of which oscillate in a narrow linewidth of <0.01 nm at ~1117 nm and ~1109 nm, respectively. The slope efficiencies are ~74% and ~93% with respect to absorbed pump power in the low-NA fibre and high-NA fibre respectively. Such high conversion efficiency suggests that very little energy is lost in the form of heat through inefficient energy transfer. Our results are supported by numerical simulations, and furthermore open up for the possibility of having narrow linewidth all-fibre laser sources in wavelength bands not traditionally covered by rare-earth doped silica fibres. Simulations also imply that this technology has the potential to produce even shorter R-DFB laser devices at the centimetre-level and with mW-level thresholds, if Bragg gratings formed in fibre materials with higher intrinsic Raman gain coefficient than silica are used. These materials include for example tellurite or chalcogenide glasses. Using glasses like these would also open up the possibility of having narrow linewidth fibre sources with DFB laser oscillating much further into the IR than what currently is possible with rare-earth doped silica glasses.

  17. Quantitation of signal transduction.

    PubMed

    Krauss, S; Brand, M D

    2000-12-01

    Conventional qualitative approaches to signal transduction provide powerful ways to explore the architecture and function of signaling pathways. However, at the level of the complete system, they do not fully depict the interactions between signaling and metabolic pathways and fail to give a manageable overview of the complexity that is often a feature of cellular signal transduction. Here, we introduce a quantitative experimental approach to signal transduction that helps to overcome these difficulties. We present a quantitative analysis of signal transduction during early mitogen stimulation of lymphocytes, with steady-state respiration rate as a convenient marker of metabolic stimulation. First, by inhibiting various key signaling pathways, we measure their relative importance in regulating respiration. About 80% of the input signal is conveyed via identifiable routes: 50% through pathways sensitive to inhibitors of protein kinase C and MAP kinase and 30% through pathways sensitive to an inhibitor of calcineurin. Second, we quantify how each of these pathways differentially stimulates functional units of reactions that produce and consume a key intermediate in respiration: the mitochondrial membrane potential. Both the PKC and calcineurin routes stimulate consumption more strongly than production, whereas the unidentified signaling routes stimulate production more than consumption, leading to no change in membrane potential despite increased respiration rate. The approach allows a quantitative description of the relative importance of signal transduction pathways and the routes by which they activate a specific cellular process. It should be widely applicable.

  18. Transduction in Bacillus subtilis.

    PubMed

    THORNE, C B

    1962-01-01

    Thorne, Curtis B. (Fort Detrick, Frederick, Md.). Transduction in Bacillus subtilis. J. Bacteriol. 83:106-111. 1962.-A bacteriophage, SP-10, isolated from soil carries out general transduction in Bacillus subtilis. Phage propagated on a streptomycin-resistant mutant of the wild-type strain W-23 was capable of transducing to prototrophy strain 168 (indole(-)), as well as all of the auxotrophic mutants of W-23-S(r) tested, which included mutants requiring arginine, histidine, adenine, guanine, thiamine, leucine, or methionine. Although strain 168 was transduced by phage SP-10, lytic activity on this strain could not be detected and attempts to propagate the phage on it failed. Transductions occurred at frequencies in the range of 10(-6) to 10(-5) per plaque-forming unit. Homologous phage was ineffective, deoxyribonuclease had no effect on the frequency of transduction, and transduction was prevented by the addition of phage antiserum. Phage SP-10 was capable of lysogenizing strain W-23-S(r), and this condition was maintained through repeated growth and sporulation cycles in potato-extract medium. Although heating at 65 C for 60 min inactivated free phage particles, spores retained their lysogenic condition after such heat treatment. When heat-treated spores of the lysogenic cultures were used as inocula for growth in a nutrient broth-yeast extract-glucose medium, filtrates contained 10(9), or more, phage particles per ml.

  19. High Quantum Efficiency OLED Lighting Systems

    SciTech Connect

    Shiang, Joseph

    2011-09-30

    The overall goal of the program was to apply improvements in light outcoupling technology to a practical large area plastic luminaire, and thus enable the product vision of an extremely thin form factor high efficiency large area light source. The target substrate was plastic and the baseline device was operating at 35 LPW at the start of the program. The target LPW of the program was a >2x improvement in the LPW efficacy and the overall amount of light to be delivered was relatively high 900 lumens. Despite the extremely difficult challenges associated with scaling up a wet solution process on plastic substrates, the program was able to make substantial progress. A small molecule wet solution process was successfully implemented on plastic substrates with almost no loss in efficiency in transitioning from the laboratory scale glass to large area plastic substrates. By transitioning to a small molecule based process, the LPW entitlement increased from 35 LPW to 60 LPW. A further 10% improvement in outcoupling efficiency was demonstrated via the use of a highly reflecting cathode, which reduced absorptive loss in the OLED device. The calculated potential improvement in some cases is even larger, ~30%, and thus there is considerable room for optimism in improving the net light coupling efficacy, provided absorptive loss mechanisms are eliminated. Further improvements are possible if scattering schemes such as the silver nanowire based hard coat structure are fully developed. The wet coating processes were successfully scaled to large area plastic substrate and resulted in the construction of a 900 lumens luminaire device.

  20. Pheromone Transduction in Moths

    PubMed Central

    Stengl, Monika

    2010-01-01

    Calling female moths attract their mates late at night with intermittent release of a species-specific sex-pheromone blend. Mean frequency of pheromone filaments encodes distance to the calling female. In their zig-zagging upwind search male moths encounter turbulent pheromone blend filaments at highly variable concentrations and frequencies. The male moth antennae are delicately designed to detect and distinguish even traces of these sex pheromones amongst the abundance of other odors. Its olfactory receptor neurons sense even single pheromone molecules and track intermittent pheromone filaments of highly variable frequencies up to about 30 Hz over a wide concentration range. In the hawkmoth Manduca sexta brief, weak pheromone stimuli as encountered during flight are detected via a metabotropic PLCβ-dependent signal transduction cascade which leads to transient changes in intracellular Ca2+ concentrations. Strong or long pheromone stimuli, which are possibly perceived in direct contact with the female, activate receptor-guanylyl cyclases causing long-term adaptation. In addition, depending on endogenous rhythms of the moth's physiological state, hormones such as the stress hormone octopamine modulate second messenger levels in sensory neurons. High octopamine levels during the activity phase maximize temporal resolution cAMP-dependently as a prerequisite to mate location. Thus, I suggest that sliding adjustment of odor response threshold and kinetics is based upon relative concentration ratios of intracellular Ca2+ and cyclic nucleotide levels which gate different ion channels synergistically. In addition, I propose a new hypothesis for the cyclic nucleotide-dependent ion channel formed by insect olfactory receptor/coreceptor complexes. Instead of being employed for an ionotropic mechanism of odor detection it is proposed to control subthreshold membrane potential oscillation of sensory neurons, as a basis for temporal encoding of odors. PMID:21228914

  1. High-efficiency concentrator silicon solar cells

    SciTech Connect

    Sinton, R.A.; Cuevas, A.; King, R.R.; Swanson, R.M. . Solid-State Electronics Lab.)

    1990-11-01

    This report presents results from extensive process development in high-efficiency Si solar cells. An advanced design for a 1.56-cm{sup 2} cell with front grids achieved 26% efficiency at 90 suns. This is especially significant since this cell does not require a prismatic cover glass. New designs for simplified backside-contact solar cells were advanced from a status of near-nonfunctionality to demonstrated 21--22% for one-sun cells in sizes up to 37.5 cm{sup 2}. An efficiency of 26% was achieved for similar 0.64-cm{sup 2} concentrator cells at 150 suns. More fundamental work on dopant-diffused regions is also presented here. The recombination vs. various process and physical parameters was studied in detail for boron and phosphorous diffusions. Emitter-design studies based solidly upon these new data indicate the performance vs design parameters for a variety of the cases of most interest to solar cell designers. Extractions of p-type bandgap narrowing and the surface recombination for p- and n-type regions from these studies have a generality that extends beyond solar cells into basic device modeling. 68 refs., 50 figs.

  2. High efficiency quadruple junction solar cells

    NASA Astrophysics Data System (ADS)

    Bestam, R.; Aissat, A.; Vilcot, J. P.

    2016-03-01

    This work focuses on the modeling and optimization of a structure based on InGaP/InGaAs/InGaAsN/Ge for photovoltaic. In this study we took into consideration the concentration effect of alloys x (In) and y (N) on the strain, the bandgap, the absorption and structure efficiency. It has been shown that the concentration of indium varies the strain and the bandgap. These two parameters change considerably the yield. Also it optimized the effect of alloys on the total absorption of the structure. For a concentration of indium x = 0.40 and y = 0.03 we had a absorption coefficient which is equal to 2 × 106 cm-1. We have found 50% efficiency for the multi-junction structure based on In0.55Ga0.45P/In0.40Ga0.60As/In0.30Ga0.70As0.97N0.03/Ge. To achieve a reliable high efficiency multi-junction structure, we just need to optimize the concentrations of different alloys.

  3. Nanooptics for high efficient photon managment

    NASA Astrophysics Data System (ADS)

    Wyrowski, Frank; Schimmel, Hagen

    2005-09-01

    Optical systems for photon management, that is the generation of tailored electromagnetic fields, constitute one of the keys for innovation through photonics. An important subfield of photon management deals with the transformation of an incident light field into a field of specified intensity distribution. In this paper we consider some basic aspects of the nature of systems for those light transformations. It turns out, that the transversal redistribution of energy (TRE) is of central concern to achieve systems with high transformation efficiency. Besides established techniques nanostructured optical elements (NOE) are demanded to implement transversal energy redistribution. That builds a bridge between the needs of photon management, optical engineering, and nanooptics.

  4. Efficient High Pressure MixtureState Equations

    NASA Technical Reports Server (NTRS)

    Harstad, K. G.; Miller, R. S.; Bellan, J.

    1996-01-01

    A method is presented for an accurate noniterative, computationally efficient calculation of high pressure fluid mixture equations of state, especially targeted to gas turbines and rocket engines. Pressures above 1 bar and temperatures above 100 K are addressed. The method is based on curve fitting an effective reference state relative to departure funcitons formed using the Peng-Robinson cubic state equation. Fit parameters for H(sub 2), O(sub 2), N(sub 2), propane, n-heptane and methanol are given.

  5. Transduction of chemical into electrical energy.

    PubMed

    Nachmansohn, D

    1976-01-01

    The paper recalls some fundamental notions, developed by Otto Meyerhof, which were used in the analysis of the transduction of chemical into mechanical energy during muscular contraction. These notions formed the basis of the approach to the analysis of the transduction of chemical into electrical energy, i.e., the very principle underlying nerve and muscle excitability and bioelectricity. Instrumental for this purpose was the use, since 1937, of electric organs of fish, a tissue highly specialized for bioelectrogenesis.

  6. Transduction of chemical into electrical energy.

    PubMed Central

    Nachmansohn, D

    1976-01-01

    The paper recalls some fundamental notions, developed by Otto Meyerhof, which were used in the analysis of the transduction of chemical into mechanical energy during muscular contraction. These notions formed the basis of the approach to the analysis of the transduction of chemical into electrical energy, i.e., the very principle underlying nerve and muscle excitability and bioelectricity. Instrumental for this purpose was the use, since 1937, of electric organs of fish, a tissue highly specialized for bioelectrogenesis. Images PMID:1061129

  7. Highly Efficient Prion Transmission by Blood Transfusion

    PubMed Central

    Andréoletti, Olivier; Litaise, Claire; Simmons, Hugh; Corbière, Fabien; Lugan, Séverine; Costes, Pierrette; Schelcher, François; Vilette, Didier; Grassi, Jacques; Lacroux, Caroline

    2012-01-01

    It is now clearly established that the transfusion of blood from variant CJD (v-CJD) infected individuals can transmit the disease. Since the number of asymptomatic infected donors remains unresolved, inter-individual v-CJD transmission through blood and blood derived products is a major public health concern. Current risk assessments for transmission of v-CJD by blood and blood derived products by transfusion rely on infectious titers measured in rodent models of Transmissible Spongiform Encephalopathies (TSE) using intra-cerebral (IC) inoculation of blood components. To address the biological relevance of this approach, we compared the efficiency of TSE transmission by blood and blood components when administrated either through transfusion in sheep or by intra-cerebral inoculation (IC) in transgenic mice (tg338) over-expressing ovine PrP. Transfusion of 200 µL of blood from asymptomatic infected donor sheep transmitted prion disease with 100% efficiency thereby displaying greater virulence than the transfusion of 200 mL of normal blood spiked with brain homogenate material containing 103ID50 as measured by intracerebral inoculation of tg338 mice (ID50 IC in tg338). This was consistent with a whole blood titer greater than 103.6 ID50 IC in tg338 per mL. However, when the same blood samples were assayed by IC inoculation into tg338 the infectious titers were less than 32 ID per mL. Whereas the transfusion of crude plasma to sheep transmitted the disease with limited efficacy, White Blood Cells (WBC) displayed a similar ability to whole blood to infect recipients. Strikingly, fixation of WBC with paraformaldehyde did not affect the infectivity titer as measured in tg338 but dramatically impaired disease transmission by transfusion in sheep. These results demonstrate that TSE transmission by blood transfusion can be highly efficient and that this efficiency is more dependent on the viability of transfused cells than the level of infectivity measured by IC

  8. Characterization of adenoviral transduction profile in prostate cancer cells and normal prostate tissue.

    PubMed

    Ai, Jianzhong; Tai, Phillip W L; Lu, Yi; Li, Jia; Ma, Hong; Su, Qin; Wei, Qiang; Li, Hong; Gao, Guangping

    2017-09-01

    Prostate diseases are common in males worldwide with high morbidity. Gene therapy is an attractive therapeutic strategy for prostate diseases, however, it is currently underdeveloped. As well known, adeno virus (Ad) is the most widely used gene therapy vector. The aims of this study are to explore transduction efficiency of Ad in prostate cancer cells and normal prostate tissue, thus further providing guidance for future prostate pathophysiological studies and therapeutic development of prostate diseases. We produced Ad expressing enhanced green fluorescence protein (EGFP), and characterized the transduction efficiency of Ad in both human and mouse prostate cancer cell lines in vitro, as well as prostate tumor xenograft, and wild-type mouse prostate tissue in vivo. Ad transduction efficiency was determined by EGFP fluorescence using microscopy and flow cytometry. Cell type-specific transduction was examined by immunofluorescence staining of cell markers. Our data showed that Ad efficiently transduced human and mouse prostate cancer cells in vitro in a dose dependent manner. Following intratumoral and intraprostate injection, Ad could efficiently transduce prostate tumor xenograft and the major prostatic cell types in vivo, respectively. Our findings suggest that Ad can efficiently transduce prostate tumor cells in vitro as well as xenograft and normal prostate tissue in vivo, and further indicate that Ad could be a potentially powerful toolbox for future gene therapy of prostate diseases. © 2017 Wiley Periodicals, Inc.

  9. Vacuum MOCVD fabrication of high efficience cells

    NASA Technical Reports Server (NTRS)

    Partain, L. D.; Fraas, L. M.; Mcleod, P. S.; Cape, J. A.

    1985-01-01

    Vacuum metal-organic-chemical-vapor-deposition (MOCVD) is a new fabrication process with improved safety and easier scalability due to its metal rather than glass construction and its uniform multiport gas injection system. It uses source materials more efficiently than other methods because the vacuum molecular flow conditions allow the high sticking coefficient reactants to reach the substrates as undeflected molecular beams and the hot chamber walls cause the low sticking coefficient reactants to bounce off the walls and interact with the substrates many times. This high source utilization reduces the materials costs power device and substantially decreases the amounts of toxic materials that must be handled as process effluents. The molecular beams allow precise growth control. With improved source purifications, vacuum MOCVD has provided p GaAs layers with 10-micron minority carrier diffusion lengths and GaAs and GaAsSb solar cells with 20% AMO efficiencies at 59X and 99X sunlight concentration ratios. Mechanical stacking has been identified as the quickest, most direct and logical path to stacked multiple-junction solar cells that perform better than the best single-junction devices. The mechanical stack is configured for immediate use in solar arrays and allows interconnections that improve the system end-of-life performance in space.

  10. Efficient high-permeability fracturing offshore

    SciTech Connect

    Phillipi, M.; Farabee, M.

    1996-12-31

    Offshore operators can more efficiently and effectively perform high-permeability and conventional hydraulic fracture treatments by blending treatment slurries under microprocessor control, adding undiluted acid on-the-fly, and altering sand concentrations and other slurry properties instantaneously. A two-skid system has been designed with these considerations in mind. The system, which can be shipped efficiently in ISO containers, has been tested on fluids up to 210-cp viscosity and can step or ramp sand concentrations up to a maximum of 20 lb/gal. All additives, including acid treatments, are added on-the-fly; leftover additives and acids may be stored for future jobs. The system may be applied in most conditions, including offshore wells requiring conventional or high-permeability fracture treatments and certain land-based wells in remote areas where a compact skid is needed. Three significant benefits have resulted from using the compact-skid system: offshore operators have been able to ship the skid system at 20% of shipping costs of non-ISO equipment; on-the-fly mixing has prevented material waste associated with batch-mixing; and volumes pumped on actual jobs have closely matched job designs. Data have been collected from several Gulf of Mexico jobs run with the two-part skid system that has been designed for conducting hydraulic fracture treatments from offshore rigs.

  11. Simple Motor Control Concept Results High Efficiency at High Velocities

    NASA Astrophysics Data System (ADS)

    Starin, Scott; Engel, Chris

    2013-09-01

    The need for high velocity motors in space applications for reaction wheels and detectors has stressed the limits of Brushless Permanent Magnet Motors (BPMM). Due to inherent hysteresis core losses, conventional BPMMs try to balance the need for torque verses hysteresis losses. Cong-less motors have significantly less hysteresis losses but suffer from lower efficiencies. Additionally, the inherent low inductance in cog-less motors result in high ripple currents or high switching frequencies, which lowers overall efficiency and increases performance demands on the control electronics.However, using a somewhat forgotten but fully qualified technology of Isotropic Magnet Motors (IMM), extremely high velocities may be achieved at low power input using conventional drive electronics. This paper will discuss the trade study efforts and empirical test data on a 34,000 RPM IMM.

  12. A Single Vector Platform for High-Level Gene Transduction of Central Neurons: Adeno-Associated Virus Vector Equipped with the Tet-Off System

    PubMed Central

    Sohn, Jaerin; Takahashi, Megumu; Okamoto, Shinichiro; Ishida, Yoko; Furuta, Takahiro

    2017-01-01

    Visualization of neurons is indispensable for the investigation of neuronal circuits in the central nervous system. Virus vectors have been widely used for labeling particular subsets of neurons, and the adeno-associated virus (AAV) vector has gained popularity as a tool for gene transfer. Here, we developed a single AAV vector Tet-Off platform, AAV-SynTetOff, to improve the gene-transduction efficiency, specifically in neurons. The platform is composed of regulator and response elements in a single AAV genome. After infection of Neuro-2a cells with the AAV-SynTetOff vector, the transduction efficiency of green fluorescent protein (GFP) was increased by approximately 2- and 15-fold relative to the conventional AAV vector with the human cytomegalovirus (CMV) or human synapsin I (SYN) promoter, respectively. We then injected the AAV vectors into the mouse neostriatum. GFP expression in the neostriatal neurons infected with the AAV-SynTetOff vector was approximately 40-times higher than that with the CMV or SYN promoter. By adding a membrane-targeting signal to GFP, the axon fibers of neostriatal neurons were clearly visualized. In contrast, by attaching somatodendritic membrane-targeting signals to GFP, axon fiber labeling was mostly suppressed. Furthermore, we prepared the AAV-SynTetOff vector, which simultaneously expressed somatodendritic membrane-targeted GFP and membrane-targeted red fluorescent protein (RFP). After injection of the vector into the neostriatum, the cell bodies and dendrites of neostriatal neurons were labeled with both GFP and RFP, whereas the axons in the projection sites were labeled only with RFP. Finally, we applied this vector to vasoactive intestinal polypeptide-positive (VIP+) neocortical neurons, one of the subclasses of inhibitory neurons in the neocortex, in layer 2/3 of the mouse primary somatosensory cortex. The results revealed the differential distribution of the somatodendritic and axonal structures at the population level. The

  13. A Single Vector Platform for High-Level Gene Transduction of Central Neurons: Adeno-Associated Virus Vector Equipped with the Tet-Off System.

    PubMed

    Sohn, Jaerin; Takahashi, Megumu; Okamoto, Shinichiro; Ishida, Yoko; Furuta, Takahiro; Hioki, Hiroyuki

    2017-01-01

    Visualization of neurons is indispensable for the investigation of neuronal circuits in the central nervous system. Virus vectors have been widely used for labeling particular subsets of neurons, and the adeno-associated virus (AAV) vector has gained popularity as a tool for gene transfer. Here, we developed a single AAV vector Tet-Off platform, AAV-SynTetOff, to improve the gene-transduction efficiency, specifically in neurons. The platform is composed of regulator and response elements in a single AAV genome. After infection of Neuro-2a cells with the AAV-SynTetOff vector, the transduction efficiency of green fluorescent protein (GFP) was increased by approximately 2- and 15-fold relative to the conventional AAV vector with the human cytomegalovirus (CMV) or human synapsin I (SYN) promoter, respectively. We then injected the AAV vectors into the mouse neostriatum. GFP expression in the neostriatal neurons infected with the AAV-SynTetOff vector was approximately 40-times higher than that with the CMV or SYN promoter. By adding a membrane-targeting signal to GFP, the axon fibers of neostriatal neurons were clearly visualized. In contrast, by attaching somatodendritic membrane-targeting signals to GFP, axon fiber labeling was mostly suppressed. Furthermore, we prepared the AAV-SynTetOff vector, which simultaneously expressed somatodendritic membrane-targeted GFP and membrane-targeted red fluorescent protein (RFP). After injection of the vector into the neostriatum, the cell bodies and dendrites of neostriatal neurons were labeled with both GFP and RFP, whereas the axons in the projection sites were labeled only with RFP. Finally, we applied this vector to vasoactive intestinal polypeptide-positive (VIP+) neocortical neurons, one of the subclasses of inhibitory neurons in the neocortex, in layer 2/3 of the mouse primary somatosensory cortex. The results revealed the differential distribution of the somatodendritic and axonal structures at the population level. The

  14. High Efficiency Targets for High Gain Inertial Confinement Fusion.

    DTIC Science & Technology

    1986-09-19

    Inertial Confinement Fusion JOHN H. GARDNER AND STEPHEN E. BODNER Laboratory for Computational Physics DTIC CD ELECTEf OCT 241986 j NU Aproedfr...81425 " 11 TITLE (include Security Classification) High Efficiency Targets for High Gain Inertial Confinement Fusion 12. PERSONAL AUTHOR(S) Gardner, John ...ArearCod) 22c OFFICE SYMBOL % John H. Gardner (202) 767-3055 Code 4040 DO FORM 1473. 84 MAR 83 APR edtion may be used until exhausted SECURITY

  15. High Efficiency, Illumination Quality OLEDs for Lighting

    SciTech Connect

    Joseph Shiang; James Cella; Kelly Chichak; Anil Duggal; Kevin Janora; Chris Heller; Gautam Parthasarathy; Jeffery Youmans; Joseph Shiang

    2008-03-31

    The goal of the program was to demonstrate a 45 lumen per watt white light device based upon the use of multiple emission colors through the use of solution processing. This performance level is a dramatic extension of the team's previous 15 LPW large area illumination device. The fundamental material system was based upon commercial polymer materials. The team was largely able to achieve these goals, and was able to deliver to DOE a 90 lumen illumination source that had an average performance of 34 LPW a 1000 cd/m{sup 2} with peak performances near 40LPW. The average color temperature is 3200K and the calculated CRI 85. The device operated at a brightness of approximately 1000cd/m{sup 2}. The use of multiple emission colors particularly red and blue, provided additional degrees of design flexibility in achieving white light, but also required the use of a multilayered structure to separate the different recombination zones and prevent interconversion of blue emission to red emission. The use of commercial materials had the advantage that improvements by the chemical manufacturers in charge transport efficiency, operating life and material purity could be rapidly incorporated without the expenditure of additional effort. The program was designed to take maximum advantage of the known characteristics of these material and proceeded in seven steps. (1) Identify the most promising materials, (2) assemble them into multi-layer structures to control excitation and transport within the OLED, (3) identify materials development needs that would optimize performance within multilayer structures, (4) build a prototype that demonstrates the potential entitlement of the novel multilayer OLED architecture (5) integrate all of the developments to find the single best materials set to implement the novel multilayer architecture, (6) further optimize the best materials set, (7) make a large area high illumination quality white OLED. A photo of the final deliverable is shown. In

  16. High-Efficiency Microwave Power Amplifier

    NASA Technical Reports Server (NTRS)

    Sims, Williams H.

    2005-01-01

    A high-efficiency power amplifier that operates in the S band (frequencies of the order of a few gigahertz) utilizes transistors operating under class-D bias and excitation conditions. Class-D operation has been utilized at lower frequencies, but, until now, has not been exploited in the S band. Nominally, in class D operation, a transistor is switched rapidly between "on" and "off" states so that at any given instant, it sustains either high current or high voltage, but not both at the same time. In the ideal case of zero "on" resistance, infinite "off" resistance, zero inductance and capacitance, and perfect switching, the output signal would be a perfect square wave. Relative to the traditional classes A, B, and C of amplifier operation, class D offers the potential to achieve greater power efficiency. In addition, relative to class-A amplifiers, class-D amplifiers are less likely to go into oscillation. In order to design this amplifier, it was necessary to derive mathematical models of microwave power transistors for incorporation into a larger mathematical model for computational simulation of the operation of a class-D microwave amplifier. The design incorporates state-of-the-art switching techniques applicable only in the microwave frequency range. Another major novel feature is a transmission-line power splitter/combiner designed with the help of phasing techniques to enable an approximation of a square-wave signal (which is inherently a wideband signal) to propagate through what would, if designed in a more traditional manner, behave as a more severely band-limited device (see figure). The amplifier includes an input, a driver, and a final stage. Each stage contains a pair of GaAs-based field-effect transistors biased in class D. The input signal can range from -10 to +10 dBm into a 50-ohm load. The table summarizes the performances of the three stages

  17. A New Highly Conserved Antibiotic Sensing/Resistance Pathway in Firmicutes Involves an ABC Transporter Interplaying with a Signal Transduction System

    PubMed Central

    Coumes-Florens, Stéphanie; Brochier-Armanet, Céline; Guiseppi, Annick; Denizot, François; Foglino, Maryline

    2011-01-01

    Signal transduction systems and ABC transporters often contribute jointly to adaptive bacterial responses to environmental changes. In Bacillus subtilis, three such pairs are involved in responses to antibiotics: BceRSAB, YvcPQRS and YxdJKLM. They are characterized by a histidine kinase belonging to the intramembrane sensing kinase family and by a translocator possessing an unusually large extracytoplasmic loop. It was established here using a phylogenomic approach that systems of this kind are specific but widespread in Firmicutes, where they originated. The present phylogenetic analyses brought to light a highly dynamic evolutionary history involving numerous horizontal gene transfers, duplications and lost events, leading to a great variety of Bce-like repertories in members of this bacterial phylum. Based on these phylogenetic analyses, it was proposed to subdivide the Bce-like modules into six well-defined subfamilies. Functional studies were performed on members of subfamily IV comprising BceRSAB from B. subtilis, the expression of which was found to require the signal transduction system as well as the ABC transporter itself. The present results suggest, for the members of this subfamily, the occurrence of interactions between one component of each partner, the kinase and the corresponding translocator. At functional and/or structural levels, bacitracin dependent expression of bceAB and bacitracin resistance processes require the presence of the BceB translocator loop. Some other members of subfamily IV were also found to participate in bacitracin resistance processes. Taken together our study suggests that this regulatory mechanism might constitute an important common antibiotic resistance mechanism in Firmicutes. [Supplemental material is available online at http://www.genome.org.] PMID:21283517

  18. Highly efficient polarization control using subwavelength high contrast transmitarrays

    NASA Astrophysics Data System (ADS)

    Arbabi, Amir; Horie, Yu; Bagheri, Mahmood; Faraon, Andrei

    2015-02-01

    We report efficient wave plates with different retardations and orientations of fast axes realized using transmitarrays composed of a periodic arrangement of amorphous silicon elliptical cylinders on glass. We show that novel polarization devices which locally rotate the polarization by different angles while preserving the wavefront can be demonstrated using such a high contrast transmitarray. We present design, fabrication and experimental characterization results for near infrared transmissive wave retarders with efficiencies in excess of 90%, and discuss the potential applications of atwill local polarization control enabled by this technology.

  19. Efficient Compression of High Resolution Climate Data

    NASA Astrophysics Data System (ADS)

    Yin, J.; Schuchardt, K. L.

    2011-12-01

    resolution climate data can be massive. Those data can consume a huge amount of disk space for storage, incur significant overhead for outputting data during simulation, introduce high latency for visualization and analysis, and may even make interactive visualization and analysis impossible given the limit of the data that a conventional cluster can handle. These problems can be alleviated by with effective and efficient data compression techniques. Even though HDF5 format supports compression, previous work has mainly focused on employ traditional general purpose compression schemes such as dictionary coder and block sorting based compression scheme. Those compression schemes mainly focus on encoding repeated byte sequences efficiently and are not well suitable for compressing climate data consist mainly of distinguished float point numbers. We plan to select and customize our compression schemes according to the characteristics of high-resolution climate data. One observation on high resolution climate data is that as the resolution become higher, values of various climate variables such as temperature and pressure, become closer in nearby cells. This provides excellent opportunities for predication-based compression schemes. We have performed a preliminary estimation of compression ratios of a very simple minded predication-based compression ratio in which we compute the difference between current float point number with previous float point number and then encoding the exponent and significance part of the float point number with entropy-based compression scheme. Our results show that we can achieve higher compression ratios between 2 and 3 in lossless compression, which is significantly higher than traditional compression algorithms. We have also developed lossy compression with our techniques. We can achive orders of magnitude data reduction while ensure error bounds. Moreover, our compression scheme is much more efficient and introduces much less overhead

  20. Novel Nanophosphors for High Efficiency Fluorescent Lamps

    SciTech Connect

    Alok Srivatava

    2007-03-31

    This is the Final Report of the Novel Nanophosphors for High Efficiency Fluorescent Lamps, Department of Energy (DOE). The overall goal of this three-year program is to develop novel hybrid phosphors by coating commercially available lamp phosphors with highly stable wide band-gap nanocrystalline phosphors (NCP). The prime technical approach is the development of NCP quantum-splitting phosphor (QSP) and ultra-violet (UV) emitting phosphors with quantum efficiencies exceeding that of the conventional phosphors at 185 nm. The novel hybrid phosphors will increase the efficiency of the fluorescent lamps by up to 32%, enabling total energy savings of 0.26 quads, the reduction in the U.S. energy bill by $6.5 billion and the reduction of the annual carbon emission by 4.1 billion kilogram. Our work started by investigating through modeling calculations the requirement for the particle size of the NCP. Our work to develop suitable nanocrystalline phosphors started with the known oxide quantum splitting and UV emitting phosphors. We demonstrated several synthesis techniques for the production of high quality nanocrystalline materials that crystallizes in the desired phase and with the desired particle size. In collaboration with our subcontractor we demonstrated the feasibility for the manufacture of NC phosphors. We also demonstrated novel techniques of coating the NCP on the surface of micron sized phosphors. Our chief achievement pertains to the successful testing of the coated hybrid phosphor systems in linear fluorescent lamps. In linear fluorescent lamp tests, we have demonstrated up to 7% increase in the efficacy of hybrid phosphors over the conventional (uncoated) phosphors. We have also demonstrated the improvement in the lumen maintenance of the coated phosphors. A hybrid phosphor system based on the commercial red emitting phosphor, Y{sub 2}O{sub 3}:Eu{sup 3+} did not show the anticipated improvement in lamp efficacy. We explored the reasons for this observation

  1. Increased sensitivity to antigen in high avidity CD8(+) T cells results from augmented membrane proximal T-cell receptor signal transduction.

    PubMed

    Sharma, Sharad K; Alexander-Miller, Martha A

    2011-07-01

    The functional avidity of a cytotoxic T lymphocyte (CTL) is known to be a critical determinant of the efficacy with which it clears pathogens. High avidity cells, which are by definition highly sensitive to peptide antigen, are superior for elimination of viruses and tumours. Our studies have established the ability of T cells to undergo avidity modulation as a result of antigen encounter. High and low avidity cells established in this manner exhibit significant differences in the amount of peptide required to elicit effector function. However, how signalling is regulated in these cells as it relates to the control of peptide sensitivity remains to be defined. To address this question, we compared T-cell receptor (TCR) signal transduction events in high and low avidity CTL generated from OT-I(rag2-) TCR transgenic mice. Our data suggest that divergent signalling is initiated at the TCR-associated CD3ζ, with low avidity CTL requiring higher amounts of pMHC to achieve threshold levels of phosphorylated CD3ζ compared with high avidity CTL. Further, this difference is transduced further downstream to mitogen-activated protein kinase and Ca(2+) signalling pathways. These results suggest that regulated control of the initiation of TCR signalling in high versus low avidity cells determines the amount of peptide required for T-cell activation. © 2011 The Authors. Immunology © 2011 Blackwell Publishing Ltd.

  2. Highly Efficient Vector-Inversion Pulse Generators

    NASA Technical Reports Server (NTRS)

    Rose, Franklin

    2004-01-01

    Improved transmission-line pulse generators of the vector-inversion type are being developed as lightweight sources of pulsed high voltage for diverse applications, including spacecraft thrusters, portable x-ray imaging systems, impulse radar systems, and corona-discharge systems for sterilizing gases. In this development, more than the customary attention is paid to principles of operation and details of construction so as to the maximize the efficiency of the pulse-generation process while minimizing the sizes of components. An important element of this approach is segmenting a pulse generator in such a manner that the electric field in each segment is always below the threshold for electrical breakdown. One design of particular interest, a complete description of which was not available at the time of writing this article, involves two parallel-plate transmission lines that are wound on a mandrel, share a common conductor, and are switched in such a manner that the pulse generator is divided into a "fast" and a "slow" section. A major innovation in this design is the addition of ferrite to the "slow" section to reduce the size of the mandrel needed for a given efficiency.

  3. Tips for selecting highly efficient cyclones

    SciTech Connect

    Amrein, D.L.

    1995-05-01

    Cyclone dust collectors have been used--and misused--all over the world for more than 100 years. One reason for the misuse is a common perception among users that all cyclones are created equal--that is, as long as a cyclone resembles a cylinder with an attached cone, it will do its job. However, to maximize separation efficiency in a specific application requires a precise cyclone design, engineered to exact fit many possible variables. A well-designed cyclone, for instance, can achieve efficiencies as high s 99.9+% when operated properly within the envelope of its specifications. Nonetheless, cyclones are often used only as first-stage filters for performing crude separations, with final collections being carried out by more-costly baghouses and scrubbers. Compared with baghouses and scrubbers, cyclones have two important considerations in their favor. One, they are almost invariably safer--in terms of the potential for generating fires and explosions--than fabric filters. Second, cyclones have lower maintenance costs since there are no filter media to replace. The paper discusses the operation, design, and troubleshooting of cyclones.

  4. High efficiency Brayton cycles using LNG

    DOEpatents

    Morrow, Charles W.

    2006-04-18

    A modified, closed-loop Brayton cycle power conversion system that uses liquefied natural gas as the cold heat sink media. When combined with a helium gas cooled nuclear reactor, achievable efficiency can approach 68 76% (as compared to 35% for conventional steam cycle power cooled by air or water). A superheater heat exchanger can be used to exchange heat from a side-stream of hot helium gas split-off from the primary helium coolant loop to post-heat vaporized natural gas exiting from low and high-pressure coolers. The superheater raises the exit temperature of the natural gas to close to room temperature, which makes the gas more attractive to sell on the open market. An additional benefit is significantly reduced costs of a LNG revaporization plant, since the nuclear reactor provides the heat for vaporization instead of burning a portion of the LNG to provide the heat.

  5. High efficiency porphyrin sensitized mesoscopic solar cells

    NASA Astrophysics Data System (ADS)

    Giordano, Fabrizio; Yi, Chenyi; Teuscher, Joël.; Zakeeruddin, Shaik M.; Grätzel, Michael

    2014-10-01

    Dye-Sensitized Solar Cells (DSSC) represents a reliable technology, ready for the market and able to compete with silicon solar cells for specific fields of application. Porphyrin dyes allow reaching high power conversion efficiency in conjunction with cobalt redox electrolytes due to larger open circuit potentials. The bigger size of the cobalt complexes compared to standard iodide/triiodide redox couple hampers its percolation through the meso-porous TiO2 network, thus impairing the regeneration process. In case of porphyrin dyes mass transport problems in the electrolyte need to be carefully handled, due to the large size of the sensitizing molecule and the bulky cobalt complexes. Herein we report the study of structural variations on porphyrin sensitizers and their influence on the DSSC performance with cobalt based redox electrolyte.

  6. High Efficiency Centrifugal Compressor for Rotorcraft Applications

    NASA Technical Reports Server (NTRS)

    Medic, Gorazd; Sharma, Om P.; Jongwook, Joo; Hardin, Larry W.; McCormick, Duane C.; Cousins, William T.; Lurie, Elizabeth A.; Shabbir, Aamir; Holley, Brian M.; Van Slooten, Paul R.

    2014-01-01

    The report "High Efficiency Centrifugal Compressor for Rotorcraft Applications" documents the work conducted at UTRC under the NRA Contract NNC08CB03C, with cost share 2/3 NASA, and 1/3 UTRC, that has been extended to 4.5 years. The purpose of this effort was to identify key technical barriers to advancing the state-of-the-art of small centrifugal compressor stages; to delineate the measurements required to provide insight into the flow physics of the technical barriers; to design, fabricate, install, and test a state-of-the-art research compressor that is representative of the rear stage of an axial-centrifugal aero-engine; and to acquire detailed aerodynamic performance and research quality data to clarify flow physics and to establish detailed data sets for future application. The design activity centered on meeting the goal set outlined in the NASA solicitation-the design target was to increase efficiency at higher work factor, while also reducing the maximum diameter of the stage. To fit within the existing Small Engine Components Test Facility at NASA Glenn Research Center (GRC) and to facilitate component re-use, certain key design parameters were fixed by UTRC, including impeller tip diameter, impeller rotational speed, and impeller inlet hub and shroud radii. This report describes the design effort of the High Efficiency Centrifugal Compressor stage (HECC) and delineation of measurements, fabrication of the compressor, and the initial tests that were performed. A new High-Efficiency Centrifugal Compressor stage with a very challenging reduction in radius ratio was successfully designed, fabricated and installed at GRC. The testing was successful, with no mechanical problems and the running clearances were achieved without impeller rubs. Overall, measured pressure ratio of 4.68, work factor of 0.81, and at design exit corrected flow rate of 3 lbm/s met the target requirements. Polytropic efficiency of 85.5 percent and stall margin of 7.5 percent were

  7. A high-efficiency superhydrophobic plasma separator.

    PubMed

    Liu, Changchun; Liao, Shih-Chuan; Song, Jinzhao; Mauk, Michael G; Li, Xuanwen; Wu, Gaoxiang; Ge, Dengteng; Greenberg, Robert M; Yang, Shu; Bau, Haim H

    2016-02-07

    To meet stringent limit-of-detection specifications for low abundance target molecules, a relatively large volume of plasma is needed for many blood-based clinical diagnostics. Conventional centrifugation methods for plasma separation are not suitable for on-site testing or bedside diagnostics. Here, we report a simple, yet high-efficiency, clamshell-style, superhydrophobic plasma separator that is capable of separating a relatively large volume of plasma from several hundred microliters of whole blood (finger-prick blood volume). The plasma separator consists of a superhydrophobic top cover with a separation membrane and a superhydrophobic bottom substrate. Unlike previously reported membrane-based plasma separators, the separation membrane in our device is positioned at the top of the sandwiched whole blood film to increase the membrane separation capacity and plasma yield. In addition, the device's superhydrophobic characteristics (i) facilitates the formation of well-defined, contracted, thin blood film with a high contact angle; (ii) minimizes biomolecular adhesion to surfaces; (iii) increases blood clotting time; and (iv) reduces blood cell hemolysis. The device demonstrated a "blood in-plasma out" capability, consistently extracting 65 ± 21.5 μL of plasma from 200 μL of whole blood in less than 10 min without electrical power. The device was used to separate plasma from Schistosoma mansoni genomic DNA-spiked whole blood with a recovery efficiency of >84.5 ± 25.8%. The S. mansoni genomic DNA in the separated plasma was successfully tested on our custom-made microfluidic chip by using loop mediated isothermal amplification (LAMP) method.

  8. Novel High Efficient Organic Photovoltaic Materials

    NASA Technical Reports Server (NTRS)

    Sun, Sam; Haliburton, James; Wang, Yi-Qing; Fan, Zhen; Taft, Charles; Maaref, Shahin; Bailey, Sheila (Technical Monitor)

    2003-01-01

    Solar energy is a renewable, nonpolluting, and most abundant energy source for human exploration of a remote site or outer space. In order to generate appreciable electrical power in space or on the earth, it is necessary to collect sunlight from large areas and with high efficiency due to the low density of sunlight. Future organic or polymer (plastic) solar cells appear very attractive due to their unique features such as light weight, flexible shape, tunability of energy band-gaps via versatile molecular or supramolecular design, synthesis, processing and device fabrication schemes, and much lower cost on large scale industrial production. It has been predicted that supramolecular and nano-phase separated block copolymer systems containing electron rich donor blocks and electron deficient acceptor blocks may facilitate the charge carrier separation and migration due to improved electronic ultrastructure and morphology in comparison to polymer composite system. This presentation will describe our recent progress in the design, synthesis and characterization of a novel block copolymer system containing donor and acceptor blocks covalently attached. Specifically, the donor block contains an electron donating alkyloxy derivatized polyphenylenevinylene (RO-PPV), the acceptor block contains an electron withdrawing alkyl-sulfone derivatized polyphenylenevinylene (SF-PPV). The key synthetic strategy includes the synthesis of each individual block first, then couple the blocks together. While the donor block has a strong PL emission at around 560 nm, and acceptor block has a strong PL emission at around 520 nm, the PL emissions of final block copolymers are severely quenched. This verifies the expected electron transfer and charge separation due to interfaces of donor and acceptor nano phase separated blocks. The system therefore has potential for variety light harvesting applications, including high efficient photovoltaic applications.

  9. Novel High Efficient Organic Photovoltaic Materials

    NASA Technical Reports Server (NTRS)

    Sun, Sam; Haliburton, James; Wang, Yi-Qing; Fan, Zhen; Taft, Charles; Maaref, Shahin; Bailey, Sheila (Technical Monitor)

    2003-01-01

    Solar energy is a renewable, nonpolluting, and most abundant energy source for human exploration of a remote site or outer space. In order to generate appreciable electrical power in space or on the earth, it is necessary to collect sunlight from large areas and with high efficiency due to the low density of sunlight. Future organic or polymer (plastic) solar cells appear very attractive due to their unique features such as light weight, flexible shape, tunability of energy band-gaps via versatile molecular or supramolecular design, synthesis, processing and device fabrication schemes, and much lower cost on large scale industrial production. It has been predicted that supramolecular and nano-phase separated block copolymer systems containing electron rich donor blocks and electron deficient acceptor blocks may facilitate the charge carrier separation and migration due to improved electronic ultrastructure and morphology in comparison to polymer composite system. This presentation will describe our recent progress in the design, synthesis and characterization of a novel block copolymer system containing donor and acceptor blocks covalently attached. Specifically, the donor block contains an electron donating alkyloxy derivatized polyphenylenevinylene (RO-PPV), the acceptor block contains an electron withdrawing alkyl-sulfone derivatized polyphenylenevinylene (SF-PPV). The key synthetic strategy includes the synthesis of each individual block first, then couple the blocks together. While the donor block has a strong PL emission at around 560 nm, and acceptor block has a strong PL emission at around 520 nm, the PL emissions of final block copolymers are severely quenched. This verifies the expected electron transfer and charge separation due to interfaces of donor and acceptor nano phase separated blocks. The system therefore has potential for variety light harvesting applications, including high efficient photovoltaic applications.

  10. A High-Efficiency Superhydrophobic Plasma Separator

    PubMed Central

    Liu, Changchun; Liao, Shih-Chuan; Song, Jinzhao; Mauk, Michael G.; Li, Xuanwen; Wu, Gaoxiang; Ge, Dengteng; Greenberg, Robert M.; Yang, Shu; Bau, Haim H.

    2016-01-01

    To meet stringent limit-of-detection specifications for low abundance target molecules, a relatively large volume of plasma is needed for many blood-based clinical diagnostics. Conventional centrifugation methods for plasma separation are not suitable for on-site testing or bedside diagnostics. Here, we report a simple, yet high-efficiency, clamshell-style, superhydrophobic plasma separator that is capable of separating a relatively large volume of plasma from several hundred microliters of whole blood (finger-prick blood volume). The plasma separator consists of a superhydrophobic top cover with a separation membrane and a superhydrophobic bottom substrate. Unlike previously reported membrane-based plasma separators, the separation membrane in our device is positioned at the top of the sandwiched whole blood film to increase the membrane separation capacity and plasma yield. In addition, the device’s superhydrophobic characteristics (i) facilitates the formation of well-defined, contracted, thin blood film with a high contact angle; (ii) minimizes biomolecular adhesion to surfaces; (iii) increases blood clotting time; and (iv) reduces blood cell hemolysis. The device demonstrated a “blood in-plasma out” capability, consistently extracting 65±21.5 μL of plasma from 200 μL of whole blood in less than 10 min without electrical power. The device was used to separate plasma from Schistosoma mansoni genomic DNA-spiked whole blood with a recovery efficiency of > 84.5 ± 25.8 %. The S. mansoni genomic DNA in the separated plasma was successfully tested on our custom-made microfluidic chip by using loop mediated isothermal amplification (LAMP) method. PMID:26732765

  11. Effective transduction of osteogenic sarcoma cells by a baculovirus vector.

    PubMed

    Song, Sun U; Shin, Seok-Hwan; Kim, Soon-Ki; Choi, Gwang-Seong; Kim, Woo-Chul; Lee, Moon-Hee; Kim, Sei-Joong; Kim, In-Ho; Choi, Mi-Sook; Hong, Young-Jin; Lee, Kwan-Hee

    2003-03-01

    Efficient gene delivery of a baculovirus-derived vector (BV-p53-lacZ) to a human osteogenic sarcoma cell line, Saos-2, was serendipitously found while evaluating the vector for gene delivery to human p53-null tumour cells in a previous study. Therefore, we investigated other human, rat and mouse osteogenic sarcoma and other types of tumour cell lines for transduction efficiency via baculovirus vectors containing a lacZ reporter gene under the control of either a cytomegalovirus or Rous sarcoma virus promoter. The expression of beta-galactosidase protein, assessed by X-Gal staining and beta-galactosidase ELISA, demonstrated an extremely high level of transduction efficiency in some osteogenic sarcoma cell lines, such as U-2OS, Saos-2 and Saos-LM2. These human osteogenic sarcoma cell lines showed levels of beta-galactosidase expression 5-40 times greater than HepG2 cells, which were previously thought to be the mammalian cells most susceptible to baculovirus-mediated gene delivery. The level of acetylated histone proteins in these tumour lines did not correlate well with the high level of reporter gene expression. These results strongly suggest that some osteogenic sarcoma cells are highly susceptible to baculovirus-mediated gene delivery and that a baculovirus-derived vector is an efficient gene delivery vehicle into human osteogenic sarcoma cells.

  12. White LED with High Package Extraction Efficiency

    SciTech Connect

    Yi Zheng; Matthew Stough

    2008-09-30

    The goal of this project is to develop a high efficiency phosphor converting (white) Light Emitting Diode (pcLED) 1-Watt package through an increase in package extraction efficiency. A transparent/translucent monolithic phosphor is proposed to replace the powdered phosphor to reduce the scattering caused by phosphor particles. Additionally, a multi-layer thin film selectively reflecting filter is proposed between blue LED die and phosphor layer to recover inward yellow emission. At the end of the project we expect to recycle approximately 50% of the unrecovered backward light in current package construction, and develop a pcLED device with 80 lm/W{sub e} using our technology improvements and commercially available chip/package source. The success of the project will benefit luminous efficacy of white LEDs by increasing package extraction efficiency. In most phosphor-converting white LEDs, the white color is obtained by combining a blue LED die (or chip) with a powdered phosphor layer. The phosphor partially absorbs the blue light from the LED die and converts it into a broad green-yellow emission. The mixture of the transmitted blue light and green-yellow light emerging gives white light. There are two major drawbacks for current pcLEDs in terms of package extraction efficiency. The first is light scattering caused by phosphor particles. When the blue photons from the chip strike the phosphor particles, some blue light will be scattered by phosphor particles. Converted yellow emission photons are also scattered. A portion of scattered light is in the backward direction toward the die. The amount of this backward light varies and depends in part on the particle size of phosphors. The other drawback is that yellow emission from phosphor powders is isotropic. Although some backward light can be recovered by the reflector in current LED packages, there is still a portion of backward light that will be absorbed inside the package and further converted to heat. Heat

  13. Tailored Materials for High Efficiency CIDI Engines

    SciTech Connect

    Grant, G.J.; Jana, S.

    2012-03-30

    The overall goal of the project, Tailored Materials for High Efficiency Compression Ignition Direct Injection (CIDI) Engines, is to enable the implementation of new combustion strategies, such as homogeneous charge compression ignition (HCCI), that have the potential to significantly increase the energy efficiency of current diesel engines and decrease fuel consumption and environmental emissions. These strategies, however, are increasing the demands on conventional engine materials, either from increases in peak cylinder pressure (PCP) or from increases in the temperature of operation. The specific objective of this project is to investigate the application of a new material processing technology, friction stir processing (FSP), to improve the thermal and mechanical properties of engine components. The concept is to modify the surfaces of conventional, low-cost engine materials. The project focused primarily on FSP in aluminum materials that are compositional analogs to the typical piston and head alloys seen in small- to mid-sized CIDI engines. Investigations have been primarily of two types over the duration of this project: (1) FSP of a cast hypoeutectic Al-Si-Mg (A356/357) alloy with no introduction of any new components, and (2) FSP of Al-Cu-Ni alloys (Alloy 339) by physically stirring-in various quantities of carbon nanotubes/nanofibers or carbon fibers. Experimental work to date on aluminum systems has shown significant increases in fatigue lifetime and stress-level performance in aluminum-silicon alloys using friction processing alone, but work to demonstrate the addition of carbon nanotubes and fibers into aluminum substrates has shown mixed results due primarily to the difficulty in achieving porosity-free, homogeneous distributions of the particulate. A limited effort to understand the effects of FSP on steel materials was also undertaken during the course of this project. Processed regions were created in high-strength, low-alloyed steels up to 0.5 in

  14. High-efficiency multiphoton boson sampling

    NASA Astrophysics Data System (ADS)

    Wang, Hui; He, Yu; Li, Yu-Huai; Su, Zu-En; Li, Bo; Huang, He-Liang; Ding, Xing; Chen, Ming-Cheng; Liu, Chang; Qin, Jian; Li, Jin-Peng; He, Yu-Ming; Schneider, Christian; Kamp, Martin; Peng, Cheng-Zhi; Höfling, Sven; Lu, Chao-Yang; Pan, Jian-Wei

    2017-06-01

    Boson sampling is considered as a strong candidate to demonstrate 'quantum computational supremacy' over classical computers. However, previous proof-of-principle experiments suffered from small photon number and low sampling rates owing to the inefficiencies of the single-photon sources and multiport optical interferometers. Here, we develop two central components for high-performance boson sampling: robust multiphoton interferometers with 99% transmission rate and actively demultiplexed single-photon sources based on a quantum dot-micropillar with simultaneously high efficiency, purity and indistinguishability. We implement and validate three-, four- and five-photon boson sampling, and achieve sampling rates of 4.96 kHz, 151 Hz and 4 Hz, respectively, which are over 24,000 times faster than previous experiments. Our architecture can be scaled up for a larger number of photons and with higher sampling rates to compete with classical computers, and might provide experimental evidence against the extended Church-Turing thesis.

  15. High efficiency diffusion molecular retention tumor targeting.

    PubMed

    Guo, Yanyan; Yuan, Hushan; Cho, Hoonsung; Kuruppu, Darshini; Jokivarsi, Kimmo; Agarwal, Aayush; Shah, Khalid; Josephson, Lee

    2013-01-01

    Here we introduce diffusion molecular retention (DMR) tumor targeting, a technique that employs PEG-fluorochrome shielded probes that, after a peritumoral (PT) injection, undergo slow vascular uptake and extensive interstitial diffusion, with tumor retention only through integrin molecular recognition. To demonstrate DMR, RGD (integrin binding) and RAD (control) probes were synthesized bearing DOTA (for (111) In(3+)), a NIR fluorochrome, and 5 kDa PEG that endows probes with a protein-like volume of 25 kDa and decreases non-specific interactions. With a GFP-BT-20 breast carcinoma model, tumor targeting by the DMR or i.v. methods was assessed by surface fluorescence, biodistribution of [(111)In] RGD and [(111)In] RAD probes, and whole animal SPECT. After a PT injection, both probes rapidly diffused through the normal and tumor interstitium, with retention of the RGD probe due to integrin interactions. With PT injection and the [(111)In] RGD probe, SPECT indicated a highly tumor specific uptake at 24 h post injection, with 352%ID/g tumor obtained by DMR (vs 4.14%ID/g by i.v.). The high efficiency molecular targeting of DMR employed low probe doses (e.g. 25 ng as RGD peptide), which minimizes toxicity risks and facilitates clinical translation. DMR applications include the delivery of fluorochromes for intraoperative tumor margin delineation, the delivery of radioisotopes (e.g. toxic, short range alpha emitters) for radiotherapy, or the delivery of photosensitizers to tumors accessible to light.

  16. High SO2 Removal Efficiency Testing

    SciTech Connect

    Blythe, Gary

    1997-07-29

    This document provides a discussion of the technical progress on DOE/PETC project number DE-AC22-92PC91338, "High Efficiency SO2 Removal Testing", for the time period 1 April through 30 June 1997. The project involves testing at six full-scale utility flue gas desulfurization (FGD) systems to evaluate low capital cost upgrades that may allow these systems to achieve up to 98% SO2 removal efficiency. The upgrades being evaluated mostly involve using performance additives in the FGD systems. The "base" project involved testing at the Tampa Electric Company's Big Bend Station. All five potential options to the base program have been exercised by DOE, involving testing at Hoosier Energy's Merom Station (Option I), Southwestern Electric Power Company's Pirkey Station (Option II), PSI Energy's Gibson Station (Option III), Duquesne Light's Elrama Station (Option IV), and New York State Electric and Gas Corporation's Kintigh Station (Option V). The originally planned testing has been completed for all six sites. However, additional testing is being conducted at the Big Bend Station. The remainder of this document is divided into four sections. Section 2, Project Summary, provides a brief overview of the status of technical efforts on this project. Section 3, Results, summarizes the outcome from technical efforts during the quarter, or results from prior quarters that have not been previously reported. In Section 4, Plans for the Next Reporting Period, an overview is provided of the technical efforts that are anticipated for the third quarter of calendar year 1997. Section 5 contains a brief acknowledgment.

  17. High bandgap III-V alloys for high efficiency optoelectronics

    DOEpatents

    Alberi, Kirstin; Mascarenhas, Angelo; Wanlass, Mark

    2017-01-10

    High bandgap alloys for high efficiency optoelectronics are disclosed. An exemplary optoelectronic device may include a substrate, at least one Al.sub.1-xIn.sub.xP layer, and a step-grade buffer between the substrate and at least one Al.sub.1-xIn.sub.xP layer. The buffer may begin with a layer that is substantially lattice matched to GaAs, and may then incrementally increase the lattice constant in each sequential layer until a predetermined lattice constant of Al.sub.1-xIn.sub.xP is reached.

  18. High concentrations of mast cell chymase facilitate the transduction of the transforming growth factor-β1/Smads signaling pathway in skin fibroblasts.

    PubMed

    Dong, Xianglin; Zhang, Chuanshan; Ma, Shaolin; Wen, Hao

    2015-03-01

    The aim of the present study was to investigate the effect of different concentrations of mast cell chymase on the transforming growth factor (TGF)-β1/Smad signaling pathway in skin fibroblasts. Cultured skin fibroblasts were treated with various concentrations of chymase for different time periods. A 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay was used to assess the rate of cell proliferation. In addition, protein expression in the fibroblasts was measured using western blot analysis. Chymase was shown to enhance the proliferation of skin fibroblasts following incubation for 48, 72 and 96 h (P<0.01). Furthermore, high concentrations of mast cell chymase were shown to enhance the mRNA and protein expression levels of TGF-β1 after long-term (≥6 h) incubation. In addition, high concentrations of mast cell chymase increased P-Smad2/3 and Smad2/3 protein expression. By contrast, low concentrations of mast cell chymase increased Smad7 protein expression. Therefore, the results demonstrated that high concentrations of mast cell chymase facilitated the transduction of the TGF-β1/Smad signaling pathway in skin fibroblasts.

  19. Novel High Efficient Organic Photovoltaic Materials

    NASA Technical Reports Server (NTRS)

    Sun, Sam; Haliburton, James; Fan, Zben; Taft, Charles; Wang, Yi-Qing; Maaref, Shahin; Mackey, Willie R. (Technical Monitor)

    2001-01-01

    In man's mission to the outer space or a remote site, the most abundant, renewable, nonpolluting, and unlimited external energy source is light. Photovoltaic (PV) materials can convert light into electrical power. In order to generate appreciable electrical power in space or on the Earth, it is necessary to collect sunlight from large areas due to the low density of sunlight, and this would be very costly using current commercially available inorganic solar cells. Future organic or polymer based solar cells seemed very attractive due to several reasons. These include lightweight, flexible shape, ultra-fast optoelectronic response time (this also makes organic PV materials attractive for developing ultra-fast photo detectors), tunability of energy band-gaps via molecular design, versatile materials synthesis and device fabrication schemes, and much lower cost on large-scale industrial production. It has been predicted that nano-phase separated block copolymer systems containing electron rich donor blocks and electron deficient acceptor blocks will facilitate the charge separation and migration due to improved electronic ultrastructure and morphology in comparison to current polymer composite photovoltaic system. This presentation will describe our recent progress in the design, synthesis and characterization of a novel donor-bridge-acceptor block copolymer system for potential high-efficient organic optoelectronic applications. Specifically, the donor block contains an electron donating alkyloxy derivatized polyphenylenevinylene, the acceptor block contains an electron withdrawing alkyl-sulfone derivatized polyphenylenevinylene, and the bridge block contains an electronically neutral non-conjugated aliphatic hydrocarbon chain. The key synthetic strategy includes the synthesis of each individual block first, then couple the blocks together. While the donor block stabilizes the holes, the acceptor block stabilizes the electrons. The bridge block is designed to hinder

  20. Novel High Efficient Organic Photovoltaic Materials

    NASA Technical Reports Server (NTRS)

    Sun, Sam; Haliburton, James; Fan, Zben; Taft, Charles; Wang, Yi-Qing; Maaref, Shahin; Mackey, Willie R. (Technical Monitor)

    2001-01-01

    In man's mission to the outer space or a remote site, the most abundant, renewable, nonpolluting, and unlimited external energy source is light. Photovoltaic (PV) materials can convert light into electrical power. In order to generate appreciable electrical power in space or on the Earth, it is necessary to collect sunlight from large areas due to the low density of sunlight, and this would be very costly using current commercially available inorganic solar cells. Future organic or polymer based solar cells seemed very attractive due to several reasons. These include lightweight, flexible shape, ultra-fast optoelectronic response time (this also makes organic PV materials attractive for developing ultra-fast photo detectors), tunability of energy band-gaps via molecular design, versatile materials synthesis and device fabrication schemes, and much lower cost on large-scale industrial production. It has been predicted that nano-phase separated block copolymer systems containing electron rich donor blocks and electron deficient acceptor blocks will facilitate the charge separation and migration due to improved electronic ultrastructure and morphology in comparison to current polymer composite photovoltaic system. This presentation will describe our recent progress in the design, synthesis and characterization of a novel donor-bridge-acceptor block copolymer system for potential high-efficient organic optoelectronic applications. Specifically, the donor block contains an electron donating alkyloxy derivatized polyphenylenevinylene, the acceptor block contains an electron withdrawing alkyl-sulfone derivatized polyphenylenevinylene, and the bridge block contains an electronically neutral non-conjugated aliphatic hydrocarbon chain. The key synthetic strategy includes the synthesis of each individual block first, then couple the blocks together. While the donor block stabilizes the holes, the acceptor block stabilizes the electrons. The bridge block is designed to hinder

  1. High efficiency crystalline silicon solar cells

    NASA Technical Reports Server (NTRS)

    Sah, C. T.

    1986-01-01

    The factors which may limit current crystalline silicon solar cells to less than 20 percent efficiency at AM 1 are investigated together with the factors which may limit the ultimate efficiency achievable. It was found that base recombination at residual defect and impurity recombination centers was the likely cause of the 20-percent efficiency barrier. Suggestions for design changes that would cut the losses due to recombinations are presented.

  2. Counterfactual quantum key distribution with high efficiency

    SciTech Connect

    Sun Ying; Wen Qiaoyan

    2010-11-15

    In a counterfactual quantum key distribution scheme, a secret key can be generated merely by transmitting the split vacuum pulses of single particles. We improve the efficiency of the first quantum key distribution scheme based on the counterfactual phenomenon. This scheme not only achieves the same security level as the original one but also has higher efficiency. We also analyze how to achieve the optimal efficiency under various conditions.

  3. Multi-petascale highly efficient parallel supercomputer

    DOEpatents

    Asaad, Sameh; Bellofatto, Ralph E.; Blocksome, Michael A.; Blumrich, Matthias A.; Boyle, Peter; Brunheroto, Jose R.; Chen, Dong; Cher, Chen -Yong; Chiu, George L.; Christ, Norman; Coteus, Paul W.; Davis, Kristan D.; Dozsa, Gabor J.; Eichenberger, Alexandre E.; Eisley, Noel A.; Ellavsky, Matthew R.; Evans, Kahn C.; Fleischer, Bruce M.; Fox, Thomas W.; Gara, Alan; Giampapa, Mark E.; Gooding, Thomas M.; Gschwind, Michael K.; Gunnels, John A.; Hall, Shawn A.; Haring, Rudolf A.; Heidelberger, Philip; Inglett, Todd A.; Knudson, Brant L.; Kopcsay, Gerard V.; Kumar, Sameer; Mamidala, Amith R.; Marcella, James A.; Megerian, Mark G.; Miller, Douglas R.; Miller, Samuel J.; Muff, Adam J.; Mundy, Michael B.; O'Brien, John K.; O'Brien, Kathryn M.; Ohmacht, Martin; Parker, Jeffrey J.; Poole, Ruth J.; Ratterman, Joseph D.; Salapura, Valentina; Satterfield, David L.; Senger, Robert M.; Smith, Brian; Steinmacher-Burow, Burkhard; Stockdell, William M.; Stunkel, Craig B.; Sugavanam, Krishnan; Sugawara, Yutaka; Takken, Todd E.; Trager, Barry M.; Van Oosten, James L.; Wait, Charles D.; Walkup, Robert E.; Watson, Alfred T.; Wisniewski, Robert W.; Wu, Peng

    2015-07-14

    A Multi-Petascale Highly Efficient Parallel Supercomputer of 100 petaOPS-scale computing, at decreased cost, power and footprint, and that allows for a maximum packaging density of processing nodes from an interconnect point of view. The Supercomputer exploits technological advances in VLSI that enables a computing model where many processors can be integrated into a single Application Specific Integrated Circuit (ASIC). Each ASIC computing node comprises a system-on-chip ASIC utilizing four or more processors integrated into one die, with each having full access to all system resources and enabling adaptive partitioning of the processors to functions such as compute or messaging I/O on an application by application basis, and preferably, enable adaptive partitioning of functions in accordance with various algorithmic phases within an application, or if I/O or other processors are underutilized, then can participate in computation or communication nodes are interconnected by a five dimensional torus network with DMA that optimally maximize the throughput of packet communications between nodes and minimize latency.

  4. Highly Efficient Contactless Electrical Energy Transmission System

    NASA Astrophysics Data System (ADS)

    Ayano, Hideki; Nagase, Hiroshi; Inaba, Hiromi

    This paper proposes a new concept for a contactless electrical energy transmission system for an elevator and an automated guided vehicle. The system has rechargeable batteries on the car and electrical energy is supplied at a specific place. When electric power is supplied to the car, it runs automatically and approaches the battery charger. Therefore, a comparatively large gap is needed between the primary transformer at the battery charger and the secondary transformer on the car in order to prevent damage which would be caused by a collision. In this case, a drop of the transformer coupling rate due to the large gap must be prevented. In conventional contactless electrical energy transmission technology, since electric power is received by a pick-up coil from a power line, a large-sized transformer is required. And when the distance over which the car runs is long, the copper loss of the line also increases. The developed system adopts a high frequency inverter using a soft switching method to miniaturize the transformer. The system has a coupling rate of 0.88 for a transformer gap length of 10mm and can operate at 91% efficiency.

  5. A high-efficiency aerothermoelastic analysis method

    NASA Astrophysics Data System (ADS)

    Wan, ZhiQiang; Wang, YaoKun; Liu, YunZhen; Yang, Chao

    2014-06-01

    In this paper, a high-efficiency aerothermoelastic analysis method based on unified hypersonic lifting surface theory is established. The method adopts a two-way coupling form that couples the structure, aerodynamic force, and aerodynamic thermo and heat conduction. The aerodynamic force is first calculated based on unified hypersonic lifting surface theory, and then the Eckert reference temperature method is used to solve the temperature field, where the transient heat conduction is solved using Fourier's law, and the modal method is used for the aeroelastic correction. Finally, flutter is analyzed based on the p-k method. The aerothermoelastic behavior of a typical hypersonic low-aspect ratio wing is then analyzed, and the results indicate the following: (1) the combined effects of the aerodynamic load and thermal load both deform the wing, which would increase if the flexibility, size, and flight time of the hypersonic aircraft increase; (2) the effect of heat accumulation should be noted, and therefore, the trajectory parameters should be considered in the design of hypersonic flight vehicles to avoid hazardous conditions, such as flutter.

  6. Energy Efficient Graphene Based High Performance Capacitors.

    PubMed

    Bae, Joonwon; Lee, Chang-Soo; Kwon, Oh Seok

    2016-10-27

    Graphene (GRP) is an interesting class of nano-structured electronic materials for various cutting-edge applications. To date, extensive research activities have been performed on the investigation of diverse properties of GRP. The incorporation of this elegant material can be very lucrative in terms of practical applications in energy storage/conversion systems. Among various those systems, high performance electrochemical capacitors (ECs) have become popular due to the recent need for energy efficient and portable devices. Therefore, in this article, the application of GRP for capacitors is described succinctly. In particular, a concise summary on the previous research activities regarding GRP based capacitors is also covered extensively. It was revealed that a lot of secondary materials such as polymers and metal oxides have been introduced to improve the performance. Also, diverse devices have been combined with capacitors for better use. More importantly, recent patents related to the preparation and application of GRP based capacitors are also introduced briefly. This article can provide essential information for future study.

  7. High-Efficiency Klystron For Television Transmitters

    NASA Technical Reports Server (NTRS)

    Ramins, Peter; Dayton, James; Mccune, Earl, Sr.; Kosmahl, Henry

    1990-01-01

    Improved klystron designed for use as final amplifier in ultrahigh-frequency (UHF) television transmitter. New device incorporates multistage depressed collector (MSDC) of advanced design to increase efficiency by recovering, from spent electron beam, some of residual kinetic energy otherwise dissipated as heat. Concept applied to increase efficiencies of microwave communication, equipment, radar systems, and particle-beam accelerators.

  8. High-Efficiency Klystron For Television Transmitters

    NASA Technical Reports Server (NTRS)

    Ramins, Peter; Dayton, James; Mccune, Earl, Sr.; Kosmahl, Henry

    1990-01-01

    Improved klystron designed for use as final amplifier in ultrahigh-frequency (UHF) television transmitter. New device incorporates multistage depressed collector (MSDC) of advanced design to increase efficiency by recovering, from spent electron beam, some of residual kinetic energy otherwise dissipated as heat. Concept applied to increase efficiencies of microwave communication, equipment, radar systems, and particle-beam accelerators.

  9. SalK/SalR, a Two-Component Signal Transduction System, Is Essential for Full Virulence of Highly Invasive Streptococcus suis Serotype 2

    PubMed Central

    Pan, Xiuzhen; Cheng, Gong; Wang, Jing; Ge, Junchao; Zheng, Feng; Cao, Min; Dong, Yaqing; Liu, Di; Wang, Jufang; Lin, Ying; Du, Hongli; Gao, George F.; Wang, Xiaoning; Hu, Fuquan; Tang, Jiaqi

    2008-01-01

    Background Streptococcus suis serotype 2 (S. suis 2, SS2) has evolved into a highly infectious entity, which caused the two recent large-scale outbreaks of human SS2 epidemic in China, and is characterized by a toxic shock-like syndrome. However, the molecular pathogenesis of this new emerging pathogen is still poorly understood. Methodology/Principal Findings 89K is a newly predicted pathogenicity island (PAI) which is specific to Chinese epidemic strains isolated from these two SS2 outbreaks. Further bioinformatics analysis revealed a unique two-component signal transduction system (TCSTS) located in the candidate 89K PAI, which is orthologous to the SalK/SalR regulatory system of Streptococcus salivarius. Knockout of salKR eliminated the lethality of SS2 in experimental infection of piglets. Functional complementation of salKR into the isogenic mutant ΔsalKR restored its soaring pathogenicity. Colonization experiments showed that the ΔsalKR mutant could not colonize any susceptible tissue of piglets when administered alone. Bactericidal assays demonstrated that resistance of the mutant to polymorphonuclear leukocyte (PMN)-mediated killing was greatly decreased. Expression microarray analysis exhibited a transcription profile alteration of 26 various genes down-regulated in the ΔsalKR mutant. Conclusions/Significance These findings suggest that SalK/SalR is requisite for the full virulence of ethnic Chinese isolates of highly pathogenic SS2, thus providing experimental evidence for the validity of this bioinformatically predicted PAI. PMID:18461172

  10. High gain high efficiency resonant DC-DC converter

    NASA Astrophysics Data System (ADS)

    Shang, Fei

    Low voltage power sources have played an important role in applications such as automotive system, renewable energy power generation and so on, where require a high gain DC-DC step-up converter. The converter is going to sustain a very high input current which can bring many design challenges in the existing topologies, such as high component current stress and power loss, complex and costly design for magnetic components, high input current ripple, etc. A new topology of high gain DCDC step-up converter proposed in this dissertation. The topology has many merits such as high gain capability, high efficiency, low components stress and requirement of the transformer, simple topology with less number of active switching devices, and easy to control. The dissertation carries out theoretical analysis of the proposed topology under different operating modes and the voltage gain has been deduced for each mode. The design of circuit components has been well studied, including the power devices current stress and power, the selection of transformer turns-ratio, the design method of the resonant tank and input current ripple. System dynamic state-space models are acquired by using generalized averaging method. Small signal model of the converter is achieved by linearization of the dynamic model around the operating points. The stability study indicates that the open loop system is stable at all operating points, except some operating points containing RHP zeros which can cause closed loop system unstable. The parameter sensitivity study shows that the system transfer function is not greatly affected by the variation of the leakage inductance and load resistance. A design of PI controller is implemented to achieve the output voltage regulation. Simulations have been carried out to validate the circuit operation and support the design analysis. A 2kW prototype has been built for experimental testing. The experimental results are in a good agreement with the theoretical

  11. High power klystrons for efficient reliable high power amplifiers

    NASA Astrophysics Data System (ADS)

    Levin, M.

    1980-11-01

    This report covers the design of reliable high efficiency, high power klystrons which may be used in both existing and proposed troposcatter radio systems. High Power (10 kW) klystron designs were generated in C-band (4.4 GHz to 5.0 GHz), S-band (2.5 GHz to 2.7 GHz), and L-band or UHF frequencies (755 MHz to 985 MHz). The tubes were designed for power supply compatibility and use with a vapor/liquid phase heat exchanger. Four (4) S-band tubes were developed in the course of this program along with two (2) matching focusing solenoids and two (2) heat exchangers. These tubes use five (5) tuners with counters which are attached to the focusing solenoids. A reliability mathematical model of the tube and heat exchanger system was also generated.

  12. AAV-mediated photoreceptor transduction of the pig cone-enriched retina

    PubMed Central

    Mussolino, C; della Corte, M; Rossi, S; Viola, F; Di Vicino, U; Marrocco, E; Neglia, S; Doria, M; Testa, F; Giovannoni, R; Crasta, M; Giunti, M; Villani, E; Lavitrano, M; Bacci, M L; Ratiglia, R; Simonelli, F; Auricchio, A; Surace, E M

    2011-01-01

    Recent success in clinical trials supports the use of adeno-associated viral (AAV) vectors for gene therapy of retinal diseases caused by defects in the retinal pigment epithelium (RPE). In contrast, evidence of the efficacy of AAV-mediated gene transfer to retinal photoreceptors, the major site of inherited retinal diseases, is less robust. In addition, although AAV-mediated RPE transduction appears efficient, independently of the serotype used and species treated, AAV-mediated photoreceptor gene transfer has not been systematically investigated thus so far in large animal models, which also may allow identifying relevant species-specific differences in AAV-mediated retinal transduction. In the present study, we used the porcine retina, which has a high cone/rod ratio. This feature allows to properly evaluate both cone and rod photoreceptors transduction and compare the transduction characteristics of AAV2/5 and 2/8, the two most efficient AAV vector serotypes for photoreceptor targeting. Here we show that AAV2/5 and 2/8 transduces both RPE and photoreceptors. AAV2/8 infects and transduces photoreceptor more efficiently than AAV2/5, similarly to what we have observed in the murine retina. The use of the photoreceptor-specific rhodopsin promoter restricts transgene expression to porcine rods and cones, and results in photoreceptor transduction levels similar to those obtained with the ubiquitous promoters tested. Finally, immunological, toxicological and biodistribution studies support the safety of AAV subretinal administration to the large porcine retina. The data presented here on AAV-mediated transduction of the cone-enriched porcine retina may affect the development of gene-based therapies for rare and common severe photoreceptor diseases. PMID:21412286

  13. High efficiency quasi-monochromatic infrared emitter

    NASA Astrophysics Data System (ADS)

    Brucoli, Giovanni; Bouchon, Patrick; Haïdar, Riad; Besbes, Mondher; Benisty, Henri; Greffet, Jean-Jacques

    2014-02-01

    Incandescent radiation sources are widely used as mid-infrared emitters owing to the lack of alternative for compact and low cost sources. A drawback of miniature hot systems such as membranes is their low efficiency, e.g., for battery powered systems. For targeted narrow-band applications such as gas spectroscopy, the efficiency is even lower. In this paper, we introduce design rules valid for very generic membranes demonstrating that their energy efficiency for use as incandescent infrared sources can be increased by two orders of magnitude.

  14. High efficiency quasi-monochromatic infrared emitter

    SciTech Connect

    Brucoli, Giovanni; Besbes, Mondher; Benisty, Henri Greffet, Jean-Jacques; Bouchon, Patrick; Haïdar, Riad

    2014-02-24

    Incandescent radiation sources are widely used as mid-infrared emitters owing to the lack of alternative for compact and low cost sources. A drawback of miniature hot systems such as membranes is their low efficiency, e.g., for battery powered systems. For targeted narrow-band applications such as gas spectroscopy, the efficiency is even lower. In this paper, we introduce design rules valid for very generic membranes demonstrating that their energy efficiency for use as incandescent infrared sources can be increased by two orders of magnitude.

  15. High Efficiency Submillimeter-Wave Imaging Array

    NASA Technical Reports Server (NTRS)

    Llombart, Nuria; Skalare, Anders; Gill, John J.; Siegel, Peter H.

    2008-01-01

    The period of a focal array is limited by the angular sampling and the f number of the system. This fact will limit the efficiency of imaging array systems to around 50%. Recently it been demonstrated that the use of a dielectric layer on top of an array of apertures can improve this efficiency limit. In this paper, we describe a similar structure that improves the efficiency in imaging applications and that it is easy to manufacture due to its compatibility with planar lithographic techniques.

  16. Vertically Aligned Graphene Sheets Membrane for Highly Efficient Solar Thermal Generation of Clean Water.

    PubMed

    Zhang, Panpan; Li, Jing; Lv, Lingxiao; Zhao, Yang; Qu, Liangti

    2017-05-23

    Efficient utilization of solar energy for clean water is an attractive, renewable, and environment friendly way to solve the long-standing water crisis. For this task, we prepared the long-range vertically aligned graphene sheets membrane (VA-GSM) as the highly efficient solar thermal converter for generation of clean water. The VA-GSM was prepared by the antifreeze-assisted freezing technique we developed, which possessed the run-through channels facilitating the water transport, high light absorption capacity for excellent photothermal transduction, and the extraordinary stability in rigorous conditions. As a result, VA-GSM has achieved average water evaporation rates of 1.62 and 6.25 kg m(-2) h(-1) under 1 and 4 sun illumination with a superb solar thermal conversion efficiency of up to 86.5% and 94.2%, respectively, better than that of most carbon materials reported previously, which can efficiently produce the clean water from seawater, common wastewater, and even concentrated acid and/or alkali solutions.

  17. Axonal-Transport-Mediated Gene Transduction in the Interior of Rat Bone

    PubMed Central

    Okabayashi, Toshitaka; Nakanishi, Kuniaki; Tsuchihara, Toyokazu; Arino, Hiroshi; Yoshihara, Yasuo; Tominaga, Susumu; Uenoyama, Maki; Suzuki, Shinya; Asagiri, Masataka; Nemoto, Koichi

    2010-01-01

    Background Gene transduction has been considered advantageous for the sustained delivery of proteins to specific target tissues. However, in the case of hard tissues, such as bone, local gene delivery remains problematic owing to anatomical accessibility limitations of the target sites. Methodology/Principal Findings Here, we evaluated the feasibility of exogenous gene transduction in the interior of bone via axonal transport following intramuscular administration of a nonviral vector. A high expression level of the transduced gene was achieved in the tibia ipsilateral to the injected tibialis anterior muscle, as well as in the ipsilateral sciatic nerve and dorsal root ganglia. In sciatic transection rats, the gene expression level was significantly lowered in bone. Conclusions/Significance These results suggest that axonal transport is critical for gene transduction. Our study may provide a basis for developing therapeutic methods for efficient gene delivery into hard tissues. PMID:20927397

  18. A high-efficiency energy conversion system

    SciTech Connect

    Belcher, A.E.

    1996-12-31

    A fundamentally new method for converting pressure into rotative motion is introduced. A historical background is given and an idealized non-turbine Brayton cycle engine and associated equations are described. Salient features are explained, together with suggested applications. Concerns over global warming, unacceptable levels of air pollution, and the need for more efficient utilization of nonrenewable energy resources, are issues which continue to plague us. The situation is further exacerbated by the possibility that underdeveloped countries, under pressure to expand their economies, might adopt power generating systems which could produce high levels of emissions. This scenario could easily develop if equipment, which once complied with stringent standards, failed to be adequately maintained through the absence of a reliable technical infrastructure. The Brayton cycle manometric engine has the potential for eliminating, or at least mitigating, many of the above issues. It is therefore of considerable importance to all populations, irrespective of demographic or economic considerations. This engine is inherently simple--the engine proper has only one moving part. It has no pistons, vanes, or other such conventional occlusive devices, yet it is a positive displacement machine. Sealing is achieved by what can best be described as a series of traveling U-tube manometers. Its construction does not require precision engineering nor the use of exotic materials, making it easy to maintain with the most rudimentary resources. Rotational velocity is low, and its normal life cycle is expected to extend to several decades. These advantages more than offset the machine`s large size. It is suited only to large and medium-scale stationary applications.

  19. High efficiency silicon solar cell review

    NASA Technical Reports Server (NTRS)

    Godlewski, M. P. (Editor)

    1975-01-01

    An overview is presented of the current research and development efforts to improve the performance of the silicon solar cell. The 24 papers presented reviewed experimental and analytic modeling work which emphasizes the improvment of conversion efficiency and the reduction of manufacturing costs. A summary is given of the round-table discussion, in which the near- and far-term directions of future efficiency improvements were discussed.

  20. Fenofibrate insulates diacylglycerol in lipid droplet/ER and preserves insulin signaling transduction in the liver of high fat fed mice.

    PubMed

    Chan, Stanley M H; Zeng, Xiao-Yi; Sun, Ruo-Qiong; Jo, Eunjung; Zhou, Xiu; Wang, Hao; Li, Songpei; Xu, Aimin; Watt, Matthew J; Ye, Ji-Ming

    2015-07-01

    Hepatic steatosis is often associated with insulin resistance as a hallmark of the metabolic syndrome in the liver. The present study investigated the effects of PPARα activation induced by fenofibrate (FB) on the relationship of insulin resistance and hepatic steatosis in mice fed a high-fat (HF) diet, which increases lipid influx into the liver. Mice were fed HF diet to induce insulin resistance and hepatic steatosis with or without FB. FB activated PPARα and ameliorated HF diet-induced glucose intolerance and hepatic insulin resistance without altering either hepatic steatosis or inflammation signaling (JNK or IKK). Interestingly, FB treatment simultaneously increased fatty acid (FA) synthesis (50%) and oxidation (66%, both p<0.01) into intermediate lipid metabolites, suggesting a FA oxidation-synthesis cycling in operation. Associated with these effects, diacylglycerols (DAGs) were sequestered within the lipid droplet/ER compartment, thus reducing their deposition in the cellular membrane, which is known to impair insulin signal transduction. These findings suggest that the reduction in membrane DAGs (rather than total hepatic steatosis) may be critical for the protection by fenofibrate-induced PPARα activation against hepatic insulin resistance induced by dietary fat.

  1. Structure and function of quinones in biological solar energy transduction: a high-frequency D-band EPR spectroscopy study of model benzoquinones.

    PubMed

    Chatterjee, Ruchira; Coates, Christopher S; Milikisiyants, Sergey; Poluektov, Oleg G; Lakshmi, K V

    2012-01-12

    Quinones are utilized as charge-transfer cofactors in a wide variety of reactions that are crucial for photosynthesis and respiration. In photosynthetic protein complexes, both Type I and Type II, including oxygenic and anoxygenic reaction centers contain quinone cofactors that are known to participate in electron- and proton-transfer processes. Type II reaction centers, purple bacterial reaction centers, and photosystem II utilize benzoquinone molecules, ubiquinone, and plastoquinone, respectively, to facilitate proton-coupled electron transfer reactions. Here, we report a systematic study of the principal components of the g-tensor of an extensive library of model benzosemiquinone anion radicals in both protic (2-isopropanol) and aprotic (dimethyl sulfoxide) solvents using high-frequency EPR spectroscopy. A detailed comparison of the experimental g-values of the benzosemiquinone models at D-band EPR frequency allows for the discrimination of substituent effects and solvent hydrogen bonds on the principal components of the g-tensor. Further, we compare the primary plastosemiquinone, Q(A)(-), of photosystem II with the substituent and solvent hydrogen bond effects of benzosemiquinone models in vitro. This study significantly extends the experimental basis for elucidating the role of both molecular structure and interactions with environment on the functional tuning of quinone cofactors in biological solar energy transduction.

  2. A transductive neuro-fuzzy controller: application to a drilling process.

    PubMed

    Gajate, Agustín; Haber, Rodolfo E; Vega, Pastora I; Alique, José R

    2010-07-01

    Recently, new neuro-fuzzy inference algorithms have been developed to deal with the time-varying behavior and uncertainty of many complex systems. This paper presents the design and application of a novel transductive neuro-fuzzy inference method to control force in a high-performance drilling process. The main goal is to study, analyze, and verify the behavior of a transductive neuro-fuzzy inference system for controlling this complex process, specifically addressing the dynamic modeling, computational efficiency, and viability of the real-time application of this algorithm as well as assessing the topology of the neuro-fuzzy system (e.g., number of clusters, number of rules). A transductive reasoning method is used to create local neuro-fuzzy models for each input/output data set in a case study. The direct and inverse dynamics of a complex process are modeled using this strategy. The synergies among fuzzy, neural, and transductive strategies are then exploited to deal with process complexity and uncertainty through the application of the neuro-fuzzy models within an internal model control (IMC) scheme. A comparative study is made of the adaptive neuro-fuzzy inference system (ANFIS) and the suggested method inspired in a transductive neuro-fuzzy inference strategy. The two neuro-fuzzy strategies are evaluated in a real drilling force control problem. The experimental results demonstrated that the transductive neuro-fuzzy control system provides a good transient response (without overshoot) and better error-based performance indices than the ANFIS-based control system. In particular, the IMC system based on a transductive neuro-fuzzy inference approach reduces the influence of the increase in cutting force that occurs as the drill depth increases, reducing the risk of rapid tool wear and catastrophic tool breakage.

  3. Ceiling culture-derived proliferative adipocytes retain high adipogenic potential suitable for use as a vehicle for gene transduction therapy.

    PubMed

    Asada, Sakiyo; Kuroda, Masayuki; Aoyagi, Yasuyuki; Fukaya, Yoshitaka; Tanaka, Shigeaki; Konno, Shunichi; Tanio, Masami; Aso, Masayuki; Satoh, Kaneshige; Okamoto, Yoshitaka; Nakayama, Toshinori; Saito, Yasushi; Bujo, Hideaki

    2011-07-01

    Adipose tissue is expected to provide a source of proliferative cells for regenerative medicine and cell-transplantation therapies using gene transfer manipulation. We have recently identified ceiling culture-derived proliferative adipocytes (ccdPAs) from the mature adipocyte fraction as cells suitable as a therapeutic gene vehicle because of their stable proliferative capacity. In this study, we examined the capability of adipogenic differentiation of the ccdPAs compared with stromal vascular fraction (SVF)-derived progenitor cells (adipose-derived stem cells, ASCs) with regard to their multipotential ability to be converted to another lineage and therefore their potential to be used for regenerative medicine research. After in vitro passaging, the surface antigen profile and the basal levels of adipogenic marker genes of the ccdPAs were not obviously different from those of the ASCs. However, the ccdPAs showed increased lipid-droplet accumulation accompanied with higher adipogenic marker gene expression after stimulation of differentiation compared with the ASCs. The higher adipogenic potential of the ccdPAs than the ASCs from the SVF was maintained for 42 days in culture. Furthermore, the difference in the adipogenic response was enhanced after partial stimulation without indomethacin. These results indicate that the ccdPAs retain a high adipogenic potential even after in vitro passaging, thus suggesting the commitment of ccdPAs to stable mature adipocytes after autotransplantation, indicating that they may have potential for use in regenerative and gene-manipulated medicine.

  4. Signal transduction and downregulation of C-MET in HGF stimulated low and highly metastatic human osteosarcoma cells.

    PubMed

    Husmann, Knut; Ducommun, Pascal; Sabile, Adam A; Pedersen, Else-Marie; Born, Walter; Fuchs, Bruno

    2015-09-04

    The poor outcome of osteosarcoma (OS), particularly in patients with metastatic disease and a five-year survival rate of only 20%, asks for more effective therapeutic strategies targeting malignancy-promoting mechanisms. Dysregulation of C-MET, its ligand hepatocyte growth factor (HGF) and the fusion oncogene product TPR-MET, first identified in human MNNG-HOS OS cells, have been described as cancer-causing factors in human cancers. Here, the expression of these molecules at the mRNA and the protein level and of HGF-stimulated signaling and downregulation of C-MET was compared in the parental low metastatic HOS and MG63 cell lines and the respective highly metastatic MNNG-HOS and 143B and the MG63-M6 and MG63-M8 sublines. Interestingly, expression of TPR-MET was only observed in MNNG-HOS cells. HGF stimulated the phosphorylation of Akt and Erk1/2 in all cell lines investigated, but phospho-Stat3 remained at basal levels. Downregulation of HGF-stimulated Akt and Erk1/2 phosphorylation was much faster in the HGF expressing MG63-M8 cells than in HOS cells. Degradation of HGF-activated C-MET occurred predominantly through the proteasomal and to a lesser extent the lysosomal pathway in the cell lines investigated. Thus, HGF-stimulated Akt and Erk1/2 signaling as well as proteasomal degradation of HGF activated C-MET are potential therapeutic targets in OS.

  5. Signal transduction and downregulation of C-MET in HGF stimulated low and highly metastatic human osteosarcoma cells

    SciTech Connect

    Husmann, Knut; Ducommun, Pascal; Sabile, Adam A.; Pedersen, Else-Marie; Born, Walter; Fuchs, Bruno

    2015-09-04

    The poor outcome of osteosarcoma (OS), particularly in patients with metastatic disease and a five-year survival rate of only 20%, asks for more effective therapeutic strategies targeting malignancy-promoting mechanisms. Dysregulation of C-MET, its ligand hepatocyte growth factor (HGF) and the fusion oncogene product TPR-MET, first identified in human MNNG-HOS OS cells, have been described as cancer-causing factors in human cancers. Here, the expression of these molecules at the mRNA and the protein level and of HGF-stimulated signaling and downregulation of C-MET was compared in the parental low metastatic HOS and MG63 cell lines and the respective highly metastatic MNNG-HOS and 143B and the MG63-M6 and MG63-M8 sublines. Interestingly, expression of TPR-MET was only observed in MNNG-HOS cells. HGF stimulated the phosphorylation of Akt and Erk1/2 in all cell lines investigated, but phospho-Stat3 remained at basal levels. Downregulation of HGF-stimulated Akt and Erk1/2 phosphorylation was much faster in the HGF expressing MG63-M8 cells than in HOS cells. Degradation of HGF-activated C-MET occurred predominantly through the proteasomal and to a lesser extent the lysosomal pathway in the cell lines investigated. Thus, HGF-stimulated Akt and Erk1/2 signaling as well as proteasomal degradation of HGF activated C-MET are potential therapeutic targets in OS. - Highlights: • Expression of TPR-MET was only observed in MNNG-HOS cells. • HGF stimulated the phosphorylation of Akt and Erk1/2 but not of Stat3 in osteosarcoma cell lines. • Degradation of HGF-activated C-MET occurred predominantly through the proteasomal pathway.

  6. High Efficiency Large Area Polysilicon Solar Cells

    NASA Technical Reports Server (NTRS)

    Johnson, S. M.; Winter, C.

    1985-01-01

    Large area (100 sq cm) polysilicon solar cells having efficiencies of up to 14.1% (100 mW/sq cm, 25 C) were fabricated and a detailed analysis was performed to identify the efficiency loss mechanisms. The 1-5 characteristics of the best cell were dominated by recombination in the quasi-neutral base due to the combination of minority carrier diffusion length and base resistivity. An analysis of the microstructural defects present in the material and their effect on the electrical properties is presented.

  7. Optimized Lentiviral Transduction Protocols by Use of a Poloxamer Enhancer, Spinoculation, and scFv-Antibody Fusions to VSV-G.

    PubMed

    Anastasov, Nataša; Höfig, Ines; Mall, Sabine; Krackhardt, Angela M; Thirion, Christian

    2016-01-01

    Lentiviral vectors (LV) are widely used to successfully transduce cells for research and clinical applications. This optimized LV infection protocol includes a nontoxic poloxamer-based adjuvant combined with antibody-retargeted lentiviral particles. The novel poloxamer P338 demonstrates superior characteristics for enhancing lentiviral transduction over the best-in-class polybrene-assisted transduction. Poloxamer P338 exhibited dual benefits of low toxicity and high efficiency of lentiviral gene delivery into a range of different primary cell cultures. One of the major advantages of P338 is its availability in pharma grade and applicability as cell culture medium additive in clinical protocols. Lentiviral vectors pseudotyped with the vesicular stomatitis virus glycoprotein (VSV-G) can be produced to high titers and mediate high transduction efficiencies in vitro. For clinical applications the need for optimized transduction protocols, especially for transduction of primary T and stem cells, is high. The successful use of retronectin, the second lentivirus enhancer available as GMP material, requires the application of specific coating protocols not applicable in all processes, and results in the need of a relatively high multiplicity of infection (MOI) to achieve effective transduction efficiencies for hematopoietic cells (e.g., CD34+ hematopoietic stem cells). Cell specificity of lentiviral vectors was successfully increased by displaying different ratios of scFv-fused VSV-G glycoproteins on the viral envelope. The system has been validated with human CD30+ lymphoma cells, resulting in preferential gene delivery to CD30+ cells, which was increased fourfold in mixed cell cultures, by presenting scFv antibody fragments binding to respective surface markers. A combination of spinoculation and poloxamer-based chemical adjuvant increases the transduction of primary T-cells by greater than twofold. The combination of poloxamer-based and scFv-retargeted LVs increased

  8. High efficiency germanium-assisted grating coupler.

    PubMed

    Yang, Shuyu; Zhang, Yi; Baehr-Jones, Tom; Hochberg, Michael

    2014-12-15

    We propose a fiber to submicron silicon waveguide vertical coupler utilizing germanium-on-silicon gratings. The germanium is epitaxially grown on silicon in the same step for building photodetectors. Coupling efficiency based on FDTD simulation is 76% at 1.55 µm and the optical 1dB bandwidth is 40 nm.

  9. The Effect of Copper and Selenium Nanocarboxylates on Biomass Accumulation and Photosynthetic Energy Transduction Efficiency of the Green Algae Chlorella Vulgaris

    NASA Astrophysics Data System (ADS)

    Mykhaylenko, Natalia F.; Zolotareva, Elena K.

    2017-02-01

    Nanoaquachelates, the nanoparticles with the molecules of water and/or carboxylic acids as ligands, are used in many fields of biotechnology. Ultra-pure nanocarboxylates of microelements are the materials of spatial perspective. In the present work, the effects of copper and selenium nanoaquachelates carboxylated with citric acid on biomass accumulation of the green algae Chlorella vulgaris were examined. Besides, the efficiency of the reactions of the light stage of photosynthesis was estimated by measuring chlorophyll a fluorescence. The addition of 0.67-4 mg L-1 of Cu nanocarboxylates resulted in the increase in Chlorella biomass by ca. 20%; however, their concentrations ranging from 20 to 40 mg L-1 strongly inhibited algal growth after the 12th day of cultivation. Se nanocarboxylates at 0.4-4 mg L-1 concentrations also stimulated the growth of C. vulgaris, and the increase in biomass came up to 40-45%. The addition of Se nanocarboxylates at smaller concentrations (0.07 or 0.2 mg L-1) at first caused the retardation of culture growth, but that effect disappeared after 18-24 days of cultivation. The addition of 2-4 mg L-1 of Cu nanocarboxylates or 0.4-4 mg L-1 of Se nanocarboxylates caused the evident initial increase in such chlorophyll a fluorescence parameters as maximal quantum yield of photosystem II photochemistry ( F v/ F m) and the quantum yield of photosystem II photochemistry in the light-adapted state ( F v'/ F m'). Photochemical fluorescence quenching coefficients declined after 24 days of growth with Cu nanocarboxylates, but they increased after 6 days of the addition of 2 or 4 mg L-1 Se nanocarboxylates. Those alterations affected the overall quantum yield of the photosynthetic electron transport in photosystem II.

  10. The Effect of Copper and Selenium Nanocarboxylates on Biomass Accumulation and Photosynthetic Energy Transduction Efficiency of the Green Algae Chlorella Vulgaris.

    PubMed

    Mykhaylenko, Natalia F; Zolotareva, Elena K

    2017-12-01

    Nanoaquachelates, the nanoparticles with the molecules of water and/or carboxylic acids as ligands, are used in many fields of biotechnology. Ultra-pure nanocarboxylates of microelements are the materials of spatial perspective. In the present work, the effects of copper and selenium nanoaquachelates carboxylated with citric acid on biomass accumulation of the green algae Chlorella vulgaris were examined. Besides, the efficiency of the reactions of the light stage of photosynthesis was estimated by measuring chlorophyll a fluorescence. The addition of 0.67-4 mg L(-1) of Cu nanocarboxylates resulted in the increase in Chlorella biomass by ca. 20%; however, their concentrations ranging from 20 to 40 mg L(-1) strongly inhibited algal growth after the 12th day of cultivation. Se nanocarboxylates at 0.4-4 mg L(-1) concentrations also stimulated the growth of C. vulgaris, and the increase in biomass came up to 40-45%. The addition of Se nanocarboxylates at smaller concentrations (0.07 or 0.2 mg L(-1)) at first caused the retardation of culture growth, but that effect disappeared after 18-24 days of cultivation. The addition of 2-4 mg L(-1) of Cu nanocarboxylates or 0.4-4 mg L(-1) of Se nanocarboxylates caused the evident initial increase in such chlorophyll a fluorescence parameters as maximal quantum yield of photosystem II photochemistry (F v/F m) and the quantum yield of photosystem II photochemistry in the light-adapted state (F v'/F m'). Photochemical fluorescence quenching coefficients declined after 24 days of growth with Cu nanocarboxylates, but they increased after 6 days of the addition of 2 or 4 mg L(-1) Se nanocarboxylates. Those alterations affected the overall quantum yield of the photosynthetic electron transport in photosystem II.

  11. Development and validation of a high-throughput cell-based screen to identify activators of a bacterial two-component signal transduction system.

    PubMed

    van Rensburg, Julia J; Fortney, Kate R; Chen, Lan; Krieger, Andrew J; Lima, Bruno P; Wolfe, Alan J; Katz, Barry P; Zhang, Zhong-Yin; Spinola, Stanley M

    2015-07-01

    CpxRA is a two-component signal transduction system (2CSTS) found in many drug-resistant Gram-negative bacteria. In response to periplasmic stress, CpxA autophosphorylates and donates a phosphoryl group to its cognate response regulator, CpxR. Phosphorylated CpxR (CpxR-P) upregulates genes involved in membrane repair and downregulates multiple genes that encode virulence factors, which are trafficked across the cell membrane. Mutants that constitutively activate CpxRA in Salmonella enterica serovar Typhimurium and Haemophilus ducreyi are avirulent in mice and humans, respectively. Thus, the activation of CpxRA has high potential as a novel antimicrobial/antivirulence strategy. Using a series of Escherichia coli strains containing a CpxR-P-responsive lacZ reporter and deletions in genes encoding CpxRA system components, we developed and validated a novel cell-based high-throughput screen (HTS) for CpxRA activators. A screen of 36,000 compounds yielded one hit compound that increased reporter activity in wild-type cells. This is the first report of a compound that activates, rather than inhibits, a 2CSTS. The activity profile of the compound against CpxRA pathway mutants in the presence of glucose suggested that the compound inhibits CpxA phosphatase activity. We confirmed that the compound induced the accumulation of CpxR-P in treated cells. Although the hit compound contained a nitro group, a derivative lacking this group retained activity in serum and had lower cytotoxicity than that of the initial hit. This HTS is amenable for the screening of larger libraries to find compounds that activate CpxRA by other mechanisms, and it could be adapted to find activators of other two-component systems. Copyright © 2015, American Society for Microbiology. All Rights Reserved.

  12. Development and Validation of a High-Throughput Cell-Based Screen To Identify Activators of a Bacterial Two-Component Signal Transduction System

    PubMed Central

    van Rensburg, Julia J.; Fortney, Kate R.; Chen, Lan; Krieger, Andrew J.; Lima, Bruno P.; Wolfe, Alan J.; Katz, Barry P.; Zhang, Zhong-yin

    2015-01-01

    CpxRA is a two-component signal transduction system (2CSTS) found in many drug-resistant Gram-negative bacteria. In response to periplasmic stress, CpxA autophosphorylates and donates a phosphoryl group to its cognate response regulator, CpxR. Phosphorylated CpxR (CpxR-P) upregulates genes involved in membrane repair and downregulates multiple genes that encode virulence factors, which are trafficked across the cell membrane. Mutants that constitutively activate CpxRA in Salmonella enterica serovar Typhimurium and Haemophilus ducreyi are avirulent in mice and humans, respectively. Thus, the activation of CpxRA has high potential as a novel antimicrobial/antivirulence strategy. Using a series of Escherichia coli strains containing a CpxR-P-responsive lacZ reporter and deletions in genes encoding CpxRA system components, we developed and validated a novel cell-based high-throughput screen (HTS) for CpxRA activators. A screen of 36,000 compounds yielded one hit compound that increased reporter activity in wild-type cells. This is the first report of a compound that activates, rather than inhibits, a 2CSTS. The activity profile of the compound against CpxRA pathway mutants in the presence of glucose suggested that the compound inhibits CpxA phosphatase activity. We confirmed that the compound induced the accumulation of CpxR-P in treated cells. Although the hit compound contained a nitro group, a derivative lacking this group retained activity in serum and had lower cytotoxicity than that of the initial hit. This HTS is amenable for the screening of larger libraries to find compounds that activate CpxRA by other mechanisms, and it could be adapted to find activators of other two-component systems. PMID:25870061

  13. Energy efficiency indicators for high electric-load buildings

    SciTech Connect

    Aebischer, Bernard; Balmer, Markus A.; Kinney, Satkartar; Le Strat, Pascale; Shibata, Yoshiaki; Varone, Frederic

    2003-06-01

    Energy per unit of floor area is not an adequate indicator for energy efficiency in high electric-load buildings. For two activities, restaurants and computer centres, alternative indicators for energy efficiency are discussed.

  14. Highly Efficient Protein Misfolding Cyclic Amplification

    PubMed Central

    Ostapchenko, Valeriy G.; Savtchenk, Regina; Alexeeva, Irina; Rohwer, Robert G.; Baskakov, Ilia V.

    2011-01-01

    Protein misfolding cyclic amplification (PMCA) provides faithful replication of mammalian prions in vitro and has numerous applications in prion research. However, the low efficiency of conversion of PrPC into PrPSc in PMCA limits the applicability of PMCA for many uses including structural studies of infectious prions. It also implies that only a small sub-fraction of PrPC may be available for conversion. Here we show that the yield, rate, and robustness of prion conversion and the sensitivity of prion detection are significantly improved by a simple modification of the PMCA format. Conducting PMCA reactions in the presence of Teflon beads (PMCAb) increased the conversion of PrPC into PrPSc from ∼10% to up to 100%. In PMCAb, a single 24-hour round consistently amplified PrPSc by 600-700-fold. Furthermore, the sensitivity of prion detection in one round (24 hours) increased by 2-3 orders of magnitude. Using serial PMCAb, a 1012-fold dilution of scrapie brain material could be amplified to the level detectible by Western blotting in 3 rounds (72 hours). The improvements in amplification efficiency were observed for the commonly used hamster 263K strain and for the synthetic strain SSLOW that otherwise amplifies poorly in PMCA. The increase in the amplification efficiency did not come at the expense of prion replication specificity. The current study demonstrates that poor conversion efficiencies observed previously have not been due to the scarcity of a sub-fraction of PrPC susceptible to conversion nor due to limited concentrations of essential cellular cofactors required for conversion. The new PMCAb format offers immediate practical benefits and opens new avenues for developing fast ultrasensitive assays and for producing abundant quantities of PrPSc in vitro. PMID:21347353

  15. High efficiency air cycle air conditioning system

    SciTech Connect

    Rannenberg, G. C.

    1985-11-19

    An air cycle air conditioning system is provided with regenerative heat exchangers upstream and downstream of an expansion turbine. A closedloop liquid circulatory system serially connects the two regenerative heat exchangers for regeneration without the bulk associated with air-to-air heat exchange. The liquid circulatory system may also provide heat transport to a remote sink heat exchanger and from a remote load as well as heat exchange within the sink heat exchanger and load for enhanced compactness and efficiency.

  16. High efficiency pump for space helium transfer

    NASA Technical Reports Server (NTRS)

    Hasenbein, Robert; Izenson, Michael G.; Swift, Walter L.; Sixsmith, Herbert

    1991-01-01

    A centrifugal pump was developed for the efficient and reliable transfer of liquid helium in space. The pump can be used to refill cryostats on orbiting satellites which use liquid helium for refrigeration at extremely low temperatures. The pump meets the head and flow requirements of on-orbit helium transfer: a flow rate of 800 L/hr at a head of 128 J/kg. The overall pump efficiency at the design point is 0.45. The design head and flow requirements are met with zero net positive suction head, which is the condition in an orbiting helium supply Dewar. The mass transfer efficiency calculated for a space transfer operation is 0.99. Steel ball bearings are used with gas fiber-reinforced teflon retainers to provide solid lubrication. These bearings have demonstrated the longest life in liquid helium endurance tests under simulated pumping conditions. Technology developed in the project also has application for liquid helium circulation in terrestrial facilities and for transfer of cryogenic rocket propellants in space.

  17. PMMA lens with high efficiency and reliability

    NASA Astrophysics Data System (ADS)

    Matsuzaki, Ichiro; Abe, Koji; Fujita, Katsuhiro

    2013-09-01

    Polymethyl Methacrylate (PMMA) Fresnel lenses are increasingly being used in concentrated photovoltaic (CPV) systems installed outdoors and, accordingly, emphasis is being placed on the durability of such lenses with regard to light transmittance when subject to ultraviolet (UV) light and dust exposure. Accelerated testing methods for evaluating durability under UV exposure were established, allowing development of a lens material with improved UV resistance. Simultaneously, through a proprietary molding method, a Fresnel lens that boasts favorable light concentration efficiency with little deformation even after prolonged outdoor use was developed. Moreover, the lens incorporates a new hard-coat finish that possesses sand durability and UV resistance comparable to that of tempered glass.

  18. High Efficiency Transverse D. C. Electron Beams.

    DTIC Science & Technology

    1984-10-01

    Research: The proposed new sintered metal oxide-metal (e.g. AI203 -Mo) cathodes have been tested. As originally predicted these cathode meterials produce...improvement over that obtained in hollow cathode lasers. These experiments show that a cw Ag laser operating at a power between 0.1 and 1 watt at...concentrated in the construction of an electron beam pumped Ag II and Cu II laser with the goal of obtaining a cw ultraviolet power of 1W at efficiencies over

  19. Quantum Confined Semiconductors for High Efficiency Photovoltaics

    NASA Astrophysics Data System (ADS)

    Beard, Matthew

    2014-03-01

    Semiconductor nanostructures, where at least one dimension is small enough to produce quantum confinement effects, provide new pathways for controlling energy flow and therefore have the potential to increase the efficiency of the primary photon-to-free energy conversion step. In this discussion, I will present the current status of research efforts towards utilizing the unique properties of colloidal quantum dots (NCs confined in three dimensions) in prototype solar cells and demonstrate that these unique systems have the potential to bypass the Shockley-Queisser single-junction limit for solar photon conversion. The solar cells are constructed using a low temperature solution based deposition of PbS or PbSe QDs as the absorber layer. Different chemical treatments of the QD layer are employed in order to obtain good electrical communication while maintaining the quantum-confined properties of the QDs. We have characterized the transport and carrier dynamics using a transient absorption, time-resolved THz, and temperature-dependent photoluminescence. I will discuss the interplay between carrier generation, recombination, and mobility within the QD layers. A unique aspect of our devices is that the QDs exhibit multiple exciton generation with an efficiency that is ~ 2 to 3 times greater than the parental bulk semiconductor.

  20. High Efficiency Thermoelectric Radioisotope Power Systems

    NASA Technical Reports Server (NTRS)

    El-Genk, Mohamed; Saber, Hamed; Caillat, Thierry

    2004-01-01

    The work performed and whose results presented in this report is a joint effort between the University of New Mexico s Institute for Space and Nuclear Power Studies (ISNPS) and the Jet Propulsion Laboratory (JPL), California Institute of Technology. In addition to the development, design, and fabrication of skutterudites and skutterudites-based segmented unicouples this effort included conducting performance tests of these unicouples for hundreds of hours to verify theoretical predictions of the conversion efficiency. The performance predictions of these unicouples are obtained using 1-D and 3-D models developed for that purpose and for estimating the actual performance and side heat losses in the tests conducted at ISNPS. In addition to the performance tests, the development of the 1-D and 3-D models and the development of Advanced Radioisotope Power systems for Beginning-Of-Life (BOM) power of 108 We are carried out at ISNPS. The materials synthesis and fabrication of the unicouples are carried out at JPL. The research conducted at ISNPS is documented in chapters 2-5 and that conducted at JP, in documented in chapter 5. An important consideration in the design and optimization of segmented thermoelectric unicouples (STUs) is determining the relative lengths, cross-section areas, and the interfacial temperatures of the segments of the different materials in the n- and p-legs. These variables are determined using a genetic algorithm (GA) in conjunction with one-dimensional analytical model of STUs that is developed in chapter 2. Results indicated that when optimized for maximum conversion efficiency, the interfacial temperatures between various segments in a STU are close to those at the intersections of the Figure-Of-Merit (FOM), ZT, curves of the thermoelectric materials of the adjacent segments. When optimizing the STUs for maximum electrical power density, however, the interfacial temperatures are different from those at the intersections of the ZT curves, but

  1. Development of high efficiency neutron detectors

    SciTech Connect

    Pickrell, M.M.; Menlove, H.O.

    1993-08-01

    We have designed a novel neutron detector system using conventional {sup 3}He detector tubes and composites of polyethylene, and graphite. At this time the design consists entirely of MCNP simulations of different detector configurations and materials. These detectors are applicable to low-level passive and active neutron assay systems such as the passive add-a-source and the {sup 252}Cf shuffler. Monte Carlo simulations of these neutron detector designs achieved efficiencies of over 35% for assay chambers that can accommodate 55-gal. drums. Only slight increases in the number of detector tubes and helium pressure are required. The detectors also have reduced die-away times. Potential applications are coincident and multiplicity neutron counting for waste disposal and safeguards. We will present the general design philosophy, underlying physics, calculation mechanics, and results.

  2. New type of transformerless high efficiency inverter

    NASA Astrophysics Data System (ADS)

    Naaijer, G. J.

    Inverter architectures are presented which allow economical ac/dc switching for solar cell array and battery power use in domestic and industrial applications. The efficiencies of currently available inverters are examined and compared with a new 2.2 kW transformerless stepped wave inverter. The inverter has low no-load losses, amounting to 200 Wh/24 hr, and features voltage steps occurring 15-30 times/sine wave period. An example is provided for an array/battery/inverter assembly with the inverter control electronics activating or disconnecting the battery subassemblies based on the total number of activated subassemblies in relation to a reference sinewave, and the need to average the battery subassembly discharge rates. A total harmonic distortion of 6 percent was observed, and the system is noted to be usable as a battery charger.

  3. High-efficiency thermoelectrics with functionalized graphene.

    PubMed

    Kim, Jeong Yun; Grossman, Jeffrey C

    2015-05-13

    Graphene superlattices made with chemical functionalization offer the possibility of tuning both the thermal and electronic properties via nanopatterning of the graphene surface. Using classical and quantum mechanical calculations, we predict that suitable chemical functionalization of graphene can introduce peaks in the density of states at the band edge that result in a large enhancement in the Seebeck coefficient, leading to an increase in the room-temperature power factor of a factor of 2 compared to pristine graphene, despite the degraded electrical conductivity. Furthermore, the presence of patterns on graphene reduces the thermal conductivity, which when taken together leads to an increase in the figure of merit for functionalized graphene by up to 2 orders of magnitude over that of pristine graphene, reaching its maximum ZT ∼ 3 at room temperature according to our calculations. These results suggest that appropriate chemical functionalization could lead to efficient graphene-based thermoelectric materials.

  4. Highly efficient multimode diode-pumped Yb:KYW laser

    NASA Astrophysics Data System (ADS)

    Kuznetsov, S. A.; Pivtsov, V. S.; Semenko, A. V.; Bagayev, S. N.

    2017-01-01

    Record high differential efficiency (53.2%) and full optical efficiency (48%) for a multimode diode-pumped Yb:KYW laser have been achieved. The characteristics of the laser and methods for improving its efficiency using a distributed Bragg reflector tapered diode laser (DBR TDL) are discussed.

  5. A review of high-efficiency silicon solar cells

    NASA Technical Reports Server (NTRS)

    Rohatgi, A.

    1986-01-01

    Various parameters that affect solar cell efficiency were discussed. It is not understood why solar cells produced from less expensive Czochralski (Cz) silicon are less efficient than cells fabricated from more expensive float-zone (Fz) silicon. Performance characteristics were presented for recently produced, high-efficient solar cells fabricated by Westinghouse Electric Corp., Spire Corp., University of New South Wales, and Stanford University.

  6. Polymer-enhanced adenoviral transduction of CAR-negative bladder cancer cells.

    PubMed

    Kasman, Laura M; Barua, Sutapa; Lu, Ping; Rege, Kaushal; Voelkel-Johnson, Christina

    2009-01-01

    The application of adenoviral gene therapy for cancer is limited by immune clearance of the virus as well as poor transduction efficiency, since the protein used for viral entry (CAR) serves physiological functions in adhesion and is frequently decreased among cancer cells. Cationic polymers have been used to enhance adenoviral gene delivery, but novel polymers with low toxicity are needed to realize this approach. We recently identified polymers that were characterized by high transfection efficiency of plasmid DNA and a low toxicity profile. In this study we evaluated the novel cationic polymer EGDE-3,3' for its potential to increase adenoviral transduction of the CAR-negative bladder cancer cell line TCCSUP. The amount of adenovirus required to transduce 50-60% of the cells was reduced 100-fold when Ad.GFP was preincubated with the EGDE-3,3' polymer. Polyethyleneimine (pEI), a positively charged polymer currently used as a standard for enhancing adenoviral transduction, also increased infectivity, but transgene expression was consistently higher with EGDE-3,3'. In addition, EGDE-3,3'-supplemented transduction of an adenovirus expressing an apoptosis inducing transgene, Ad.GFP-TRAIL, significantly enhanced the amount of cell death. Thus, our results indicate that novel biocompatible polymers may be useful in improving the delivery of adenoviral gene therapy.

  7. Development of high-efficiency solar cells on silicon web

    NASA Technical Reports Server (NTRS)

    Rohatgi, A.; Meier, D. L.; Campbell, R. B.; Seidensticker, R. G.; Rai-Choudhury, P.

    1985-01-01

    High-efficiency dendritic cells were discussed. The influence of twin planes and heat treatment on the location and effect of trace impurities was of particular interest. Proper heat treatment often increases efficiency by causing impurities to pile up at twin planes. Oxide passivation had a beneficial effect on efficiency. A very efficient antireflective (AR) coating of zinc selenide and magnesium fluoride was designed and fabricated. An aluminum back-surface reflector was also effective.

  8. Preparation of highly efficient manganese catalase mimics.

    PubMed

    Triller, Michael U; Hsieh, Wen-Yuan; Pecoraro, Vincent L; Rompel, Annette; Krebs, Bernt

    2002-10-21

    The series of compounds [Mn(bpia)(mu-OAc)](2)(ClO(4))(2) (1), [Mn(2)(bpia)(2)(muO)(mu-OAc)](ClO(4))(3).CH(3)CN (2), [Mn(bpia)(mu-O)](2)(ClO(4))(2)(PF(6)).2CH(3)CN (3), [Mn(bpia)(Cl)(2)](ClO)(4) (4), and [(Mn(bpia)(Cl))(2)(mu-O)](ClO(4))(2).2CH(3)CN (5) (bpia = bis(picolyl)(N-methylimidazol-2-yl)amine) represents a structural, spectroscopic, and functional model system for manganese catalases. Compounds 3 and 5 have been synthesized from 2 via bulk electrolysis and ligand exchange, respectively. All complexes have been structurally characterized by X-ray crystallography and by UV-vis and EPR spectroscopies. The different bridging ligands including the rare mono-mu-oxo and mono-mu-oxo-mono-mu-carboxylato motifs lead to a variation of the Mn-Mn separation across the four binuclear compounds of 1.50 A (Mn(2)(II,II) = 4.128 A, Mn(2)(III,III) = 3.5326 and 3.2533 A, Mn(2)(III,IV) = 2.624 A). Complexes 1, 2, and 3 are mimics for the Mn(2)(II,II), the Mn(2)(III,III), and the Mn(2)(III,IV) oxidation states of the native enzyme. UV-vis spectra of these compounds show similarities to those of the corresponding oxidation states of manganese catalase from Thermus thermophilus and Lactobacillus plantarum. Compound 2 exhibits a rare example of a Jahn-Teller compression. While complexes 1 and 3 are efficient catalysts for the disproportionation of hydrogen peroxide and contain an N(4)O(2) donor set, 4 and 5 show no catalase activity. These complexes have an N(4)Cl(2) and N(4)OCl donor set, respectively, and serve as mimics for halide inhibited manganese catalases. Cyclovoltammetric data show that the substitution of oxygen donor atoms with chloride causes a shift of redox potentials to more positive values. To our knowledge, complex 1 is the most efficient binuclear functional manganese catalase mimic exhibiting saturation kinetics to date.

  9. Efficient high-capacity steganography technique

    NASA Astrophysics Data System (ADS)

    Abdulla, Alan A.; Jassim, Sabah A.; Sellahewa, Harin

    2013-05-01

    Performance indicators characterizing modern steganographic techniques include capacity (i.e. the quantity of data that can be hidden in the cover medium), stego quality (i.e. artifacts visibility), security (i.e. undetectability), and strength or robustness (intended as the resistance against active attacks aimed to destroy the secret message). Fibonacci based embedding techniques have been researched and proposed in the literature to achieve efficient steganography in terms of capacity with respect to stego quality. In this paper, we investigated an innovative idea that extends Fibonacci-like steganography by bit-plane(s) mapping instead of bit-plane(s) replacement. Our proposed algorithm increases embedding capacity using bit-plane mapping to embed two bits of the secret message in three bits of a pixel of the cover, at the expense of a marginal loss in stego quality. While existing Fibonacci embedding algorithms do not use certain intensities of the cover for embedding due to the limitation imposed by the Zeckendorf theorem, our proposal solve this problem and make all intensity values candidates for embedding. Experimental results demonstrate that the proposed technique double the embedding capacity when compared to existing Fibonacci methods, and it is secure against statistical attacks such as RS, POV, and difference image histogram (DIH).

  10. The augmentation of intracellular delivery of peptide therapeutics by artificial protein transduction domains.

    PubMed

    Yoshikawa, Tomoaki; Sugita, Toshiki; Mukai, Yohei; Abe, Yasuhiro; Nakagawa, Shinsaku; Kamada, Haruhiko; Tsunoda, Shin-ichi; Tsutsumi, Yasuo

    2009-07-01

    Protein transduction domains (PTDs), such as HIV-derived Tat, have been successfully used as functional biomaterials for intracellular delivery of anti-cancer macromolecular drugs (protein, peptides, and oligonucleotides). Although there were therefore great expectations regarding the therapeutic potential of PTDs for the development of anti-cancer therapeutics, their clinical application so far has been extremely limited because of the relatively high concentrations required to mediate any effects on cancer cells in vitro or in vivo. In this context, improving the transduction efficiency of PTDs using phage display-based molecular evolution techniques may be useful for creating artificial PTDs with high efficiency and safety. Here, we report an evaluation of transduction efficiency and toxicity of such artificial PTDs (designated mT02 and mT03) compared with Tat. The internalization of mT02 was the most rapid and efficient by a mechanism different from the usual macropinocytosis. Furthermore, we found that artificial PTDs fused with survivin antagonistic peptide potentiate tumor cell-cytostatic activity. Thus, the results of this work provide new insights for designing new-generation peptide therapeutics for a wide variety of cancers as well as those expressing survivin.

  11. High Thermal Efficiency in Airplane Service

    NASA Technical Reports Server (NTRS)

    Sparrow, S W

    1920-01-01

    Described here is a method by which high average fuel economy has been achieved in aircraft engines. Details are given of the design of certain foreign engines that employ an unusual type of fuel-air ratio control in which the change in power produced by a mixture change is due almost entirely to the change in the power producing ability of the unit weight of the mixture. The safety and performance features of this type of control are explained.

  12. High Efficiency Micromachining System Applied in Nanolithography

    NASA Astrophysics Data System (ADS)

    Chen, Xing; Lee, Dong Weon; Choi, Young Soo

    Scanning probe lithography such as direct-writing lithographic processes and nanoscratching techniques based on scanning probe microscopy have presented new micromachining methods for microelectromechanical system (MEMS). In this paper, a micromachining system for thermal scanning probe lithography is introduced, which consists of the cantilever arrays and a big stroke micro XY-stage. A large machining area and high machining speed can be realized by combining arrays of cantilevers possessing sharp tips at their top with the novel micro XY-stage which can obtain big displacements under relatively low driving voltage and in a small size. According to the above configuration, this micromachining system is provided with high throughputs and suitable for industrialization due to its MEMS-based simple fabrication process. The novel micro XY-stage applied in this system is presented in detail including the unique structure and principles, which shows an obvious improvement and distinct advantages in comparison with traditional structures. It is analyzed by mathematical model and then simulated using finite element method (FEM), it is proved to be able to practically serve the micromachining system with high capability.

  13. Highly efficient retinal metabolism in cones

    PubMed Central

    Miyazono, Sadaharu; Shimauchi-Matsukawa, Yoshie; Tachibanaki, Shuji; Kawamura, Satoru

    2008-01-01

    After bleaching of visual pigment in vertebrate photoreceptors, all-trans retinal is reduced to all-trans retinol by retinol dehydrogenases (RDHs). We investigated this reaction in purified carp rods and cones, and we found that the reducing activity toward all-trans retinal in the outer segment (OS) of cones is >30 times higher than that of rods. The high activity of RDHs was attributed to high content of RDH8 in cones. In the inner segment (IS) in both rods and cones, RDH8L2 and RDH13 were found to be the major enzymes among RDH family proteins. We further found a previously undescribed and effective pathway to convert 11-cis retinol to 11-cis retinal in cones: this oxidative conversion did not require NADP+ and instead was coupled with reduction of all-trans retinal to all-trans retinol. The activity was >50 times effective than the oxidizing activity of RDHs that require NADP+. These highly effective reactions of removal of all-trans retinal by RDH8 and production of 11-cis retinal by the coupling reaction are probably the underlying mechanisms that ensure effective visual pigment regeneration in cones that function under much brighter light conditions than rods. PMID:18836074

  14. High efficiency radioisotope thermophotovoltaic prototype generator

    NASA Technical Reports Server (NTRS)

    Avery, James E.; Samaras, John E.; Fraas, Lewis M.; Ewell, Richard

    1995-01-01

    250 Watts of heat input, we expect this prototype to produce over 300 Watts of electrical energy output for a system energy conversion efficiency of over 12%. This low risk, near term design provides advances relative to present radioisotope thermophotovoltaic generators and has the additional advantage of allowing component and system development and testing to begin immediately. Improved cells and filters can easily be incorporated in this baseline system if they should become available in the future.

  15. Biologically inspired highly efficient buoyancy engine

    NASA Astrophysics Data System (ADS)

    Akle, Barbar; Habchi, Wassim; Abdelnour, Rita; Blottman, John, III; Leo, Donald

    2012-04-01

    Undersea distributed networked sensor systems require a miniaturization of platforms and a means of both spatial and temporal persistence. One aspect of this system is the necessity to modulate sensor depth for optimal positioning and station-keeping. Current approaches involve pneumatic bladders or electrolysis; both require mechanical subsystems and consume significant power. These are not suitable for the miniaturization of sensor platforms. Presented in this study is a novel biologically inspired method that relies on ionic motion and osmotic pressures to displace a volume of water from the ocean into and out of the proposed buoyancy engine. At a constant device volume, the displaced water will alter buoyancy leading to either sinking or floating. The engine is composed of an enclosure sided on the ocean's end by a Nafion ionomer and by a flexible membrane separating the water from a gas enclosure. Two electrodes are placed one inside the enclosure and the other attached to the engine on the outside. The semi-permeable membrane Nafion allows water motion in and out of the enclosure while blocking anions from being transferred. The two electrodes generate local concentration changes of ions upon the application of an electrical field; these changes lead to osmotic pressures and hence the transfer of water through the semi-permeable membrane. Some aquatic organisms such as pelagic crustacean perform this buoyancy control using an exchange of ions through their tissue to modulate its density relative to the ambient sea water. In this paper, the authors provide an experimental proof of concept of this buoyancy engine. The efficiency of changing the engine's buoyancy is calculated and optimized as a function of electrode surface area. For example electrodes made of a 3mm diameter Ag/AgCl proved to transfer approximately 4mm3 of water consuming 4 Joules of electrical energy. The speed of displacement is optimized as a function of the surface area of the Nafion

  16. Inverse zonation of hepatocyte transduction with AAV vectors between mice and non-human primates

    PubMed Central

    Bell, Peter; Wang, Lili; Gao, Guangping; Haskins, Mark E.; Tarantal, Alice F.; McCarter, Robert J.; Zhu, Yanqing; Yu, Hongwei; Wilson, James M.

    2011-01-01

    Gene transfer vectors based on adeno-associated virus 8 (AAV8) are highly efficient in liver transduction and can be easily administered by intravenous injection. In mice, AAV8 transduces predominantly hepatocytes near central veins and yields lower transduction levels in hepatocytes in periportal regions. This transduction bias has important implications for gene therapy that aims to correct metabolic liver enzymes because metabolic zonation along the porto-central axis requires the expression of therapeutic proteins within the zone where they are normally localized. In the present study we compared the expression pattern of AAV8 expressing green fluorescent protein (GFP) in liver between mice, dogs, and non-human primates. We confirmed the pericentral dominance in transgene expression in mice with AAV8 when the liver-specific thyroid hormone-binding globulin (TBG) promoter was used but also observed the same expression pattern with the ubiquitous chicken β-actin (CB) and cytomegalovirus (CMV) promoters, suggesting that transduction zonation is not caused by promoter specificity. Predominantly pericentral expression was also found in dogs injected with AAV8. In contrast, in cynomolgus and rhesus macaques the expression pattern from AAV vectors was reversed, i.e. transgene expression was most intense around portal areas and less intense or absent around central veins. Infant rhesus macaques as well as newborn mice injected with AAV8 however showed a random distribution of transgene expression with neither portal nor central transduction bias. Based on the data in monkeys, adult humans treated with AAV vectors are predicted to also express transgenes predominantly in periportal regions whereas infants are likely to show a uniform transduction pattern in liver. PMID:21778099

  17. High Efficiency, High Density Terrestrial Panel. [for solar cell modules

    NASA Technical Reports Server (NTRS)

    Wohlgemuth, J.; Wihl, M.; Rosenfield, T.

    1979-01-01

    Terrestrial panels were fabricated using rectangular cells. Packing densities in excess of 90% with panel conversion efficiencies greater than 13% were obtained. Higher density panels can be produced on a cost competitive basis with the standard salami panels.

  18. 40 CFR 761.71 - High efficiency boilers.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 40 Protection of Environment 32 2013-07-01 2013-07-01 false High efficiency boilers. 761.71... PROHIBITIONS Storage and Disposal § 761.71 High efficiency boilers. (a) To burn mineral oil dielectric fluid containing a PCB concentration of ≥50 ppm, but boiler shall comply with the...

  19. 40 CFR 761.71 - High efficiency boilers.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 40 Protection of Environment 30 2010-07-01 2010-07-01 false High efficiency boilers. 761.71... PROHIBITIONS Storage and Disposal § 761.71 High efficiency boilers. (a) To burn mineral oil dielectric fluid containing a PCB concentration of ≥50 ppm, but boiler shall comply with the...

  20. 40 CFR 761.71 - High efficiency boilers.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 40 Protection of Environment 31 2014-07-01 2014-07-01 false High efficiency boilers. 761.71... PROHIBITIONS Storage and Disposal § 761.71 High efficiency boilers. (a) To burn mineral oil dielectric fluid containing a PCB concentration of ≥50 ppm, but boiler shall comply with the...

  1. High efficiency low cost GaAs/Ge cell technology

    NASA Technical Reports Server (NTRS)

    Ho, Frank

    1990-01-01

    Viewgraphs on high efficiency low cost GaAs/Ge cell technology are presented. Topics covered include: high efficiency, low cost GaAs/Ge solar cells; advantages of Ge; comparison of typical production cells for space applications; panel level comparisons; and solar cell technology trends.

  2. High efficiency low cost GaAs/Ge cell technology

    NASA Technical Reports Server (NTRS)

    Ho, Frank

    1990-01-01

    Viewgraphs on high efficiency low cost GaAs/Ge cell technology are presented. Topics covered include: high efficiency, low cost GaAs/Ge solar cells; advantages of Ge; comparison of typical production cells for space applications; panel level comparisons; and solar cell technology trends.

  3. Some approaches for fabricating high-efficiency OLEDs

    NASA Astrophysics Data System (ADS)

    Jou, Jwo-Huei; Wang, Wei-Ben; Shen, Shih-Ming; Wu, Ming-Hsuan

    2009-08-01

    High-efficiency is strongly desired for organic light-emitting diodes (OLEDs) to be fully realized as the future display and lighting technology. To replace current illumination tools, such as incandescent bulbs and fluorescent tubes, for examples, OLEDs with much higher efficiency are demanded. We will present herein some approaches for fabricating high-efficiency OLEDs of blue and white emission. Besides employing highly efficient electroluminescent guests and thin device architecture, low injection barriers to carriers, high carrier-transporting character, effective carrier/exciton confinement, balanced carrier-injection, exciton generation on host, effective host-to-guest energy-transfer and improved light-coupling efficiency are essential. Amongst, the incorporation of nano-dots in emissive- and non-emissive-layers can markedly improve the device efficiency. The enhancement is especially marked as small polymeric nano-dots are incorporated into the non-emissive layers. Since the incorporation is not in the emissive layer, the efficiency improvement mechanism works for both fluorescent and phosphorescent devices. Importantly, the efficiency improvement is also a strong function of the surface charge density of the nano-dots. Regardless positively or negatively charged, the improvement becomes more pronounced as the charge density increases. Results regarding some lately achieved extraordinarily highly-efficient OLEDs containing nano-dots with high surface charge will be presented.

  4. Optogenetics - Bringing light into the darkness of mammalian signal transduction.

    PubMed

    Mühlhäuser, Wignand W D; Fischer, Adrian; Weber, Wilfried; Radziwill, Gerald

    2017-02-01

    Cells receive many different environmental clues to which they must adapt accordingly. Therefore, a complex signal transduction network has evolved. Cellular signal transduction is a highly dynamic process, in which the specific outcome is a result of the exact spatial and temporal resolution of single sub-events. While conventional techniques, like chemical inducer systems, have led to a sound understanding of the architecture of signal transduction pathways, the spatiotemporal aspects were often impossible to resolve. Optogenetics, based on genetically encoded light-responsive proteins, has the potential to revolutionize manipulation of signal transduction processes. Light can be easily applied with highest precision and minimal invasiveness. This review focuses on examples of optogenetic systems which were generated and applied to manipulate non-neuronal mammalian signaling processes at various stages of signal transduction, from cell membrane through cytoplasm to nucleus. Further, the future of optogenetic signaling will be discussed. Copyright © 2016 Elsevier B.V. All rights reserved.

  5. HIGH EFFICIENCY DESULFURIZATION OF SYNTHESIS GAS

    SciTech Connect

    Anirban Mukherjee; Kwang-Bok Yi; Elizabeth J. Podlaha; Douglas P. Harrison

    2001-11-01

    Mixed metal oxides containing CeO{sub 2} and ZrO{sub 2} are being studied as high temperature desulfurization sorbents capable of achieving the DOE Vision 21 target of 1 ppmv of less H{sub 2}S. The research is justified by recent results in this laboratory that showed that reduced CeO{sub 2}, designated CeO{sub n} (1.5 < n < 2.0), is capable of achieving the 1 ppmv target in highly reducing gas atmospheres. The addition of ZrO{sub 2} has improved the performance of oxidation catalysts and three-way automotive catalysts containing CeO{sub 2}, and should have similar beneficial effects on CeO{sub 2} desulfurization sorbents. An electrochemical method for synthesizing CeO{sub 2}-ZrO{sub 2} has been developed and the products have been characterized by XRD and TEM during year 01. Nanocrystalline particles having a diameter of about 5 nm and containing from approximately 10 mol% to 80 mol% ZrO{sub 2} have been prepared. XRD showed the product to be a solid solution at low ZrO{sub 2} contents with a separate ZrO{sub 2} phase emerging at higher ZrO{sub 2} levels. Phase separation did not occur when the solid solutions were heat treated at 700 C. A flow reactor system constructed of quartz and teflon has been constructed, and a gas chromatograph equipped with a pulsed flame photometric detector (PFPD) suitable for measuring sub-ppmv levels of H{sub 2}S has been purchased with LSU matching funds. Preliminary desulfurization tests using commercial CeO{sub 2} and CeO{sub 2}-ZrO{sub 2} in highly reducing gas compositions has confirmed that CeO{sub 2}-ZrO{sub 2} is more effective than CeO{sub 2} in removing H{sub 2}S. At 700 C the product H{sub 2}S concentration using CeO{sub 2}-ZrO{sub 2} sorbent was near the 0.1 ppmv PFPD detection limit during the prebreakthrough period.

  6. Compact and highly efficient laser pump cavity

    DOEpatents

    Chang, Jim J.; Bass, Isaac L.; Zapata, Luis E.

    1999-01-01

    A new, compact, side-pumped laser pump cavity design which uses non-conventional optics for injection of laser-diode light into a laser pump chamber includes a plurality of elongated light concentration channels. In one embodiment, the light concentration channels are compound parabolic concentrators (CPC) which have very small exit apertures so that light will not escape from the pumping chamber and will be multiply reflected through the laser rod. This new design effectively traps the pump radiation inside the pump chamber that encloses the laser rod. It enables more uniform laser pumping and highly effective recycle of pump radiation, leading to significantly improved laser performance. This new design also effectively widens the acceptable radiation wavelength of the diodes, resulting in a more reliable laser performance with lower cost.

  7. 2250-MHz High Efficiency Microwave Power Amplifier (HEMPA)

    NASA Technical Reports Server (NTRS)

    Sims, W. Herbert; Bell, Joseph L. (Technical Monitor)

    2001-01-01

    Tnis paper will focus on developing an exotic switching technique that enhances the DC-to-RF conversion efficiency of microwave power amplifiers. For years, switching techniques implemented in the 10 kHz to 30 MHz region have resulted in DC-to-RF conversion efficiencies of 90-95-percent. Currently amplifier conversion efficiency, in the 2-3 GHz region approaches, 10-20-percent. Using a combination of analytical modeling and hardware testing, a High Efficiency Microwave Power Amplifier was built that demonstrated conversion efficiencies four to five times higher than current state of the art.

  8. HIGH EFFICIENCY DESULFURIZATION OF SYNTHESIS GAS

    SciTech Connect

    Kwang-Bok Yi; Anirban Mukherjee; Elizabeth J. Podlaha; Douglas P. Harrison

    2004-03-01

    Mixed metal oxides containing ceria and zirconia have been studied as high temperature desulfurization sorbents with the objective of achieving the DOE Vision 21 target of 1 ppmv or less H{sub 2}S in the product gas. The research was justified by recent results in this laboratory that showed that reduced CeO{sub 2}, designated CeOn (1.5 < n < 2.0), is capable of achieving the 1 ppmv target in highly reducing gas atmospheres. The addition of ZrO{sub 2} has improved the performance of oxidation catalysts and three-way automotive catalysts containing CeO{sub 2}, and was postulated to have similar beneficial effects on CeO{sub 2} desulfurization sorbents. An electrochemical method for synthesizing CeO{sub 2}-ZrO{sub 2} mixtures was developed and the products were characterized by XRD and TEM during year 01. Nanocrystalline particles having a diameter of about 5 nm and containing from approximately 10 mol% to 80 mol% ZrO{sub 2} were prepared. XRD analysis showed the product to be a solid solution at low ZrO{sub 2} contents with a separate ZrO{sub 2} phase emerging at higher ZrO{sub 2} levels. Unfortunately, the quantity of CeO{sub 2}-ZrO{sub 2} that could be prepared electrochemically was too small to permit desulfurization testing. Also during year 01 a laboratory-scale fixed-bed reactor was constructed for desulfurization testing. All components of the reactor and analytical systems that were exposed to low concentrations of H{sub 2}S were constructed of quartz, Teflon, or silcosteel. Reactor product gas composition as a function of time was determined using a Varian 3800 gas chromatograph equipped with a pulsed flame photometric detector (PFPD) for measuring low H{sub 2}S concentrations from approximately 0.1 to 10 ppmv, and a thermal conductivity detector (TCD) for higher concentrations of H{sub 2}S. Larger quantities of CeO{sub 2}-ZrO{sub 2} mixtures from other sources, including mixtures prepared in this laboratory using a coprecipitation procedure, were obtained

  9. Energy efficient engine high-pressure turbine detailed design report

    NASA Technical Reports Server (NTRS)

    Thulin, R. D.; Howe, D. C.; Singer, I. D.

    1982-01-01

    The energy efficient engine high-pressure turbine is a single stage system based on technology advancements in the areas of aerodynamics, structures and materials to achieve high performance, low operating economics and durability commensurate with commercial service requirements. Low loss performance features combined with a low through-flow velocity approach results in a predicted efficiency of 88.8 for a flight propulsion system. Turbine airfoil durability goals are achieved through the use of advanced high-strength and high-temperature capability single crystal materials and effective cooling management. Overall, this design reflects a considerable extension in turbine technology that is applicable to future, energy efficient gas-turbine engines.

  10. HIGH EFFICIENCY DESULFURIZATION OF SYNTHESIS GAS

    SciTech Connect

    Kwang-Bok Yi; Elizabeth J. Podlaha; Douglas P. Harrison

    2003-11-01

    Mixed metal oxides containing CeO{sub 2} and ZrO{sub 2} are being studied as high temperature desulfurization sorbents capable of achieving the DOE Vision 21 target of 1 ppmv or less H{sub 2}S. The research is justified by recent results in this laboratory that showed that reduced CeO{sub 2}, designated CeOn (1.5 < n < 2.0), is capable of achieving the 1 ppmv target in highly reducing gas atmospheres. The addition of ZrO{sub 2} has improved the performance of oxidation catalysts and three-way automotive catalysts containing CeO{sub 2}, and should have similar beneficial effects on CeO{sub 2} desulfurization sorbents. An electrochemical method for synthesizing CeO{sub 2}-ZrO{sub 2} was developed and the products were characterized by XRD and TEM during year 01. Nanocrystalline particles having a diameter of about 5 nm and containing from approximately 10 mol% to 80 mol% ZrO{sub 2} were prepared. XRD analysis showed the product to be a solid solution at low ZrO{sub 2} contents with a separate ZrO{sub 2} phase emerging at higher ZrO{sub 2} levels. Unfortunately, the quantity of CeO{sub 2}-ZrO{sub 2} that could be prepared electrochemically was too small to permit full desulfurization testing. Also during year 01 a laboratory-scale fixed-bed reactor was constructed for desulfurization testing. All components of the reactor and analytical systems that may be exposed to low concentrations of H{sub 2}S are constructed of quartz, Teflon, or silcosteel. Reactor product gas composition as a function of time is determined using a Varian 3800 gas chromatograph equipped with a pulsed flame photometric detector (PFPD) for measuring low H{sub 2}S concentrations (<{approx}10 ppmv) and a thermal conductivity detector (TCD) for higher concentrations of H{sub 2}S. Larger quantities of CeO{sub 2}-ZrO{sub 2} mixtures from other sources, including mixtures prepared in this laboratory using a coprecipitation procedure, have been obtained. Much of the work during year 02 consisted of

  11. HIGH EFFICIENCY DESULFURIZATION OF SYNTHESIS GAS

    SciTech Connect

    Kwang-Bok Yi; Elizabeth J. Podlaha; Douglas P. Harrison

    2002-11-01

    Mixed metal oxides containing CeO{sub 2} and ZrO{sub 2} are being studied as high temperature desulfurization sorbents capable of achieving the DOE Vision 21 target of 1 ppmv or less H{sub 2}S. The research is justified by recent results in this laboratory that showed that reduced CeO{sub 2}, designated CeO{sub n} (1.5 < n < 2.0), is capable of achieving the 1 ppmv target in highly reducing gas atmospheres. The addition of ZrO{sub 2} has improved the performance of oxidation catalysts and three-way automotive catalysts containing CeO{sub 2}, and should have similar beneficial effects on CeO{sub 2} desulfurization sorbents. An electrochemical method for synthesizing CeO{sub 2}-ZrO{sub 2} was developed and the products were characterized by XRD and TEM during year 01. Nanocrystalline particles having a diameter of about 5 nm and containing from approximately 10 mol% to 80 mol% ZrO{sub 2} were prepared. XRD showed the product to be a solid solution at low ZrO{sub 2} contents with a separate ZrO{sub 2} phase emerging at higher ZrO{sub 2} levels. Unfortunately, the quantity of CeO{sub 2}-ZrO{sub 2} that could be prepared electrochemically was too small to permit full testing in our desulfurization reactor. Also during year 01 a laboratory-scale fixed-bed reactor was constructed for desulfurization testing. All components of the reactor and analytical systems that may be exposed to low concentrations of H{sub 2}S are constructed of quartz, Teflon, or silcosteel. Reactor product gas composition as a function of time is determined using a Varian 3800 gas chromatograph equipped with a pulsed flame photometric detector (PFPD) for measuring low H{sub 2}S concentrations ({approx}< 10 ppmv) and a thermal conductivity detector (TCD) for higher concentrations of H{sub 2}S. Larger quantities of CeO{sub 2}-ZrO{sub 2} mixtures from other sources, including mixtures prepared in this laboratory using a coprecipitation procedure, have been obtained. Characterization and desulfurization

  12. High Efficiency Gene Transfer to Airways of Mice Using Influenza Hemagglutinin Pseudotyped Lentiviral Vectors

    PubMed Central

    Patel, Manij; Giddings, Angela M.; Sechelski, John; Olsen, John C.

    2014-01-01

    Background A limitation to efficient lentivirus-mediated airway gene transfer is the lack of receptors to commonly used viral envelopes on the luminal surface of airway epithelia. The use of viral envelopes with natural tropism to the airway could be useful for overcoming this limitation. Methods We investigated influenza hemagglutinin (HA) pseudotyped EIAV-derived lentiviral vector-mediated gene transfer to the airway epithelium of adult and newborn mice. For these studies high-titer vectors were delivered by intranasal administration. In addition, we tested the feasibility of vector re-dosing to the nasal airway. Results Delivery of high-titer HA pseudotyped lentiviral vectors by nasal administration to newborn mouse pups or adult mice results in efficient transduction of airway epithelial cells in the nose, trachea, and lungs. In the nose vector expression was predominant in the respiratory epithelium and was not observed in the olfactory epithelium. In the trachea and large airways of the lung approximately 46% and 40%, respectively, of surface epithelial cells could be transduced. The efficiency of re-dosing to the nasal airway of mice was found to be dependent upon the age of the animal when the first dose is administered and the length of time between doses. Conclusions A single intranasal dose of concentrated influenza HA-pseudotyped lentiviral vector is sufficient for efficient gene transfer to the airways of mice. This is a promising result that could lead to the development of effective gene transfer reagents for the treatment of cystic fibrosis and other human lung diseases. PMID:23319179

  13. Comparative study of p38 MAPK signal transduction pathway of peripheral blood mononuclear cells from patients with coal-combustion-type fluorosis with and without high hair selenium levels.

    PubMed

    Chen, Qun; Wang, Zhilun; Xiong, Yongmin; Zou, Xiuzhen; Liu, Zhengwen

    2010-09-01

    Coal-combustion-type fluorosis has only been reported in China and its pathogenesis has not been fully understood. Fluoride causes chronic toxic effects and selenium modulates cellular activities through signal transduction in human cells. The present study enrolled three groups of subjects with well-defined serum and urine fluoride and hair selenium: high fluoride+high selenium group, high fluoride group and normal control group. The expressions of p38, NF-kB p65, caspase-3 and p53 genes at both protein and mRNA levels in peripheral blood mononuclear cells (PBMCs) were determined by Western blotting and quantitative real-time RT-PCR, respectively. The results showed that the expressions of p38, NF-kB p65, and caspase-3 protein in high fluoride group were higher than those in the other two groups. The mRNA level of NF-kB p65 and caspase-3 was significantly higher in high fluoride+high selenium group than control and lower than high fluoride group. The mRNA and protein level of p53 was significantly higher in high fluoride+high selenium group than that in other two groups. These results suggest that selenium may influence the protein and gene expression associated with p38 signal transduction pathway and up-regulate p53 expression in PBMCs from patients with coal-combustion-type fluorosis.

  14. Summary of high-efficiency solar-cell research

    NASA Technical Reports Server (NTRS)

    Kachare, R.

    1985-01-01

    High-efficiency solar-cell activities supporting efforts to achieve the DOE Five-Year Plan goals are summarized. Specific objectives are to identify and resolve key generic problems that limit cell efficiency to below theoretically predicted values and to design and fabricate cells having efficiences equal to or greater than 20% (AM1.5). Theoretical curves for various p-n junction cells were shown. The effects of practical barriers on cell efficiency was depicted along with the modeling parameters. Cell design parameters used in the analyses were described. The usefulness and present limitations of the existing modeling capabilities were presented. The historical evolution of the efficiencies of cells made from web and edge-defined film-fed growth (EFG) silicon ribbons were also described. The status of contemporary higher-efficiency technical capabilities and future activities to raise efficiencies were stated.

  15. Improved Intravitreal AAV-Mediated Inner Retinal Gene Transduction after Surgical Internal Limiting Membrane Peeling in Cynomolgus Monkeys.

    PubMed

    Takahashi, Kazuhisa; Igarashi, Tsutomu; Miyake, Koichi; Kobayashi, Maika; Yaguchi, Chiemi; Iijima, Osamu; Yamazaki, Yoshiyuki; Katakai, Yuko; Miyake, Noriko; Kameya, Shuhei; Shimada, Takashi; Takahashi, Hiroshi; Okada, Takashi

    2017-01-04

    The retina is an ideal target for gene therapy because of its easy accessibility and limited immunological response. We previously reported that intravitreally injected adeno-associated virus (AAV) vector transduced the inner retina with high efficiency in a rodent model. In large animals, however, the efficiency of retinal transduction was low, because the vitreous and internal limiting membrane (ILM) acted as barriers to transduction. To overcome these barriers in cynomolgus monkeys, we performed vitrectomy (VIT) and ILM peeling before AAV vector injection. Following intravitreal injection of 50 μL triple-mutated self-complementary AAV serotype 2 vector encoding EGFP, transduction efficiency was analyzed. Little expression of GFP was detected in the control and VIT groups, but in the VIT+ILM group, strong GFP expression was detected within the peeled ILM area. To detect potential adverse effects, we monitored the retinas using color fundus photography, optical coherence tomography, and electroretinography. No serious side effects associated with the pretreatment were observed. These results indicate that surgical ILM peeling before AAV vector administration would be safe and useful for efficient transduction of the nonhuman primate retina and provide therapeutic benefits for the treatment of retinal diseases.

  16. High efficiency endocrine operation protocol: From design to implementation.

    PubMed

    Mascarella, Marco A; Lahrichi, Nadia; Cloutier, Fabienne; Kleiman, Simcha; Payne, Richard J; Rosenberg, Lawrence

    2016-10-01

    We developed a high efficiency endocrine operative protocol based on a mathematical programming approach, process reengineering, and value-stream mapping to increase the number of operations completed per day without increasing operating room time at a tertiary-care, academic center. Using this protocol, a case-control study of 72 patients undergoing endocrine operation during high efficiency days were age, sex, and procedure-matched to 72 patients undergoing operation during standard days. The demographic profile, operative times, and perioperative complications were noted. The average number of cases per 8-hour workday in the high efficiency and standard operating rooms were 7 and 5, respectively. Mean procedure times in both groups were similar. The turnaround time (mean ± standard deviation) in the high efficiency group was 8.5 (±2.7) minutes as compared with 15.4 (±4.9) minutes in the standard group (P < .001). Transient postoperative hypocalcemia was 6.9% (5/72) and 8.3% (6/72) for the high efficiency and standard groups, respectively (P = .99). In this study, patients undergoing high efficiency endocrine operation had similar procedure times and perioperative complications compared with the standard group. The proposed high efficiency protocol seems to better utilize operative time and decrease the backlog of patients waiting for endocrine operation in a country with a universal national health care program. Copyright © 2016 Elsevier Inc. All rights reserved.

  17. A Drosophila mechanosensory transduction channel.

    PubMed

    Walker, R G; Willingham, A T; Zuker, C S

    2000-03-24

    Mechanosensory transduction underlies a wide range of senses, including proprioception, touch, balance, and hearing. The pivotal element of these senses is a mechanically gated ion channel that transduces sound, pressure, or movement into changes in excitability of specialized sensory cells. Despite the prevalence of mechanosensory systems, little is known about the molecular nature of the transduction channels. To identify such a channel, we analyzed Drosophila melanogaster mechanoreceptive mutants for defects in mechanosensory physiology. Loss-of-function mutations in the no mechanoreceptor potential C (nompC) gene virtually abolished mechanosensory signaling. nompC encodes a new ion channel that is essential for mechanosensory transduction. As expected for a transduction channel, D. melanogaster NOMPC and a Caenorhabditis elegans homolog were selectively expressed in mechanosensory organs.

  18. High brilliance and high efficiency: optimized high power diode laser bars

    NASA Astrophysics Data System (ADS)

    Hülsewede, R.; Schulze, H.; Sebastian, J.; Schröder, D.; Meusel, J.; Hennig, P.

    2008-02-01

    The strong increasing laser market has ongoing demands to reduce the costs of diode laser pumped systems. For that reason JENOPTIK Diode Lab GmbH (JDL) optimized the bar brilliance (small vertical far field divergence) and bar efficiency (higher optical power operation) with respect to the pump applications. High efficiency reduces the costs for mounting and cooling and high brilliance increases the coupling efficiency. Both are carefully adjusted in the 9xx nm - high power diode laser bars for pump applications in disc- and fiber lasers. Based on low loss waveguide structures high brilliance bars with 19° fast axis beam divergence (FWHM) with 58 % maximum efficiency and 27° fast axis beam divergence (FWHM) with 62 % maximum efficiency are developed. Mounted on conductive cooled heat sinks high power operation with lifetime > 20.000 hours at 120 W output power level (50 % filling factor bars) and 80W (20 % filling factor bars) is demonstrated. 808nm bars used as pump sources for Nd:YAG solid state lasers are still dominating in the market. With respect to the demands on high reliability at high power operation current results of a 100 W high power life time test are showing more than 9000 hour operation time for passively cooled packaged high efficiency 50 % filling factor bars. Measurement of the COMD-level after this hard pulse life time test demonstrates very high power levels with no significant droop in COMD-power level. This confirms the high facet stability of JDL's facet technology. New high power diode laser bars with wavelength of 825 nm and 885 nm are still under development and first results are presented.

  19. High efficiency IMPATT diodes for 60 GHz intersatellite link applications

    NASA Technical Reports Server (NTRS)

    Haugland, E. J.

    1984-01-01

    Intersatellite links are expected to play an increasingly important role in future satellite systems. Improved components are required to properly utilize the wide bandwidth allocated for intersatellite link applications around 60 GHz. IMPATT diodes offer the highest potential performance as solid state power sources for a 60 GHz transmitter. Presently available devices do not have the desired power and efficiency. High efficiency, high power IMPATT diodes for intersatellite link applications are being developed by NASA and other government agencies. This paper describes the development of high efficiency 60 GHz IMPATT diodes by NASA. These programs are cofunded by the U.S. Air Force, Space Division.

  20. High efficiency IMPATT diodes for 60 GHz intersatellite link applications

    NASA Technical Reports Server (NTRS)

    Haugland, E. J.

    1984-01-01

    Intersatellite links are expected to play an increasingly important role in future satellite systems. Improved components are required to properly utilize the wide bandwidth allocated for intersatellite link applications around 60 GHz. IMPATT diodes offer the highest potential performance as solid state power sources for a 60 GHz transmitter. Presently available devices do not have the desired power and efficiency. High efficiency, high power IMPATT diodes for intersatellite link applications are being developed by NASA and other government agencies. The development of high efficiency 60 GHz IMPATT diodes by NASA is described.

  1. High-efficiency backlight module with two guiding modes.

    PubMed

    Li, Chang-Yi; Pan, Jui-Wen

    2014-03-10

    We propose a design for a high-efficiency backlight module that does not require a brightness enhancement film (BEF). With the high-efficiency backlight module it is possible to achieve almost the same half-luminance angle as a conventional edge-lit backlight module can achieve. The backlight system is comprised of a crisscross light guide plate (LGP) and one diffuser sheet. The crisscross LGP is composed of a LGP and optically patterned film (OPF). The backlight module allows light to be extracted through the direct guiding mode and top guiding mode, respectively. We controlled arrangement of the microstructures to increase the optical efficiency and the uniformity by two modes. Compared to the conventional edge-lit backlight module, there is a two-fold improvement in both the total optical efficiency and on-axis luminance with the high-efficiency backlight module.

  2. Development of High Efficient Organic Thin-film Solar Cells

    NASA Astrophysics Data System (ADS)

    Hiramoto, Masahiro

    Fundamental principles and p-i-n junction concept of organic solar cells are described. Methods for improvement of conversion efficiency such as nanostructure design of co-deposited i-layer and high-purification of organic semiconductors are explained. Conversion efficiency exceeding 5% was observed. Cell operation for 1000 hours (42 days) was successfully accomplished.

  3. Efficient High Performance Collective Communication for Distributed Memory Environments

    ERIC Educational Resources Information Center

    Ali, Qasim

    2009-01-01

    Collective communication allows efficient communication and synchronization among a collection of processes, unlike point-to-point communication that only involves a pair of communicating processes. Achieving high performance for both kernels and full-scale applications running on a distributed memory system requires an efficient implementation of…

  4. Development of an Improved High Efficiency Thin Solar Cell

    NASA Technical Reports Server (NTRS)

    Lindmayer, J.; Wrigley, C.; Storti, G.

    1979-01-01

    High efficiency cells (up to 14 AMO at 25 C)were fabricated from 10 - 15 ohm-cm silicon by using screen printed aluminum paste as the alloy source for the production of back surface fields. Thick consistency pastes that have been cured prior to a short heat treatment at 850 C were most effective in achieving these efficiency levels.

  5. Space solar cells: High efficiency and radiation damage

    NASA Technical Reports Server (NTRS)

    Brandhorst, H., Jr.; Bernatowicz, D. T.

    1980-01-01

    The progress and status of efforts to increase the end-of-life efficiency of solar cells for space use is assessed. High efficiency silicon solar cells, silicon solar cell radiation damage, GaAs solar cell performance and radiation damage and 30 percent devices are discussed.

  6. High efficiency hydrocarbon-free resonance transition potassium laser

    NASA Astrophysics Data System (ADS)

    Zweiback, Jason; Hager, Gordon; Krupke, William F.

    2009-05-01

    We experimentally demonstrate a high efficiency potassium laser using a 0.15 nm bandwidth alexandrite laser as the pump source. The laser uses naturally occurring helium as the buffer gas. We achieve a 64% slope efficiency and a 57% optical to optical conversion. A pulsed laser model shows good agreement with the data.

  7. Efficient High Performance Collective Communication for Distributed Memory Environments

    ERIC Educational Resources Information Center

    Ali, Qasim

    2009-01-01

    Collective communication allows efficient communication and synchronization among a collection of processes, unlike point-to-point communication that only involves a pair of communicating processes. Achieving high performance for both kernels and full-scale applications running on a distributed memory system requires an efficient implementation of…

  8. Vpx mediated degradation of SAMHD1 has only a very limited effect on lentiviral transduction rate in ex vivo cultured HSPCs☆

    PubMed Central

    Li, Duo; Schlaepfer, Erika; Audigé, Annette; Rochat, Mary-Aude; Ivic, Sandra; Knowlton, Caitlin N.; Kim, Baek; Keppler, Oliver T.; Speck, Roberto F.

    2016-01-01

    Understanding how to achieve efficient transduction of hematopoietic stem and progenitor cells (HSPCs), while preserving their long-term ability to self-reproduce, is key for applying lentiviral-based gene engineering methods. SAMHD1 is an HIV-1 restriction factor in myeloid and resting CD4+ T cells that interferes with reverse transcription by decreasing the nucleotide pools or by its RNase activity. Here we show that SAMHD1 is expressed at high levels in HSPCs cultured in a medium enriched with cytokines. Thus, we hypothesized that degrading SAMHD1 in HSPCs would result in more efficient lentiviral transduction rates. We used viral like particles (VLPs) containing Vpx, shRNA against SAMHD1, or provided an excess of dNTPs or dNs to study this question. Regardless of the method applied, we saw no increase in the lentiviral transduction rate. The result was different when we used viruses (HR-GFP-Vpx+) which carry Vpx and encode GFP. These viruses allow assessment of the effects of Vpx specifically in the transduced cells. Using HR-GFP-Vpx+ viruses, we observed a modest but significant increase in the transduction efficiency. These data suggest that SAMHD1 has some limited efficacy in blocking reverse transcription but the major barrier for efficient lentiviral transduction occurs before reverse transcription. PMID:26207584

  9. High efficiency interdigitated back contact silicon solar cells

    NASA Astrophysics Data System (ADS)

    Verlinden, P.; van de Wiele, F.; Stehelin, G.; Floret, F.; David, J. P.

    Interdigitated back contact (IBC) silicon solar cells with 25.6 percent efficiency at 10 W/sq cm and 24.4 percent at 30 W/ sq cm were fabricated. The authors report on the technological process, which produces a high effective carrier lifetime in the bulk (780 microsec), and on the characterization of the cells. The front side of these cells is textured and has an efficient polka-dot floating tandem junction. IBC and point-contact (PC) cells are fabricated on the same substrate and their efficiencies are compared. The possiblity of reaching 29 percent efficiency at 300X is shown.

  10. High-efficiency photonic crystal solar cell architecture.

    PubMed

    Chutinan, Alongkarn; Kherani, Nazir P; Zukotynski, Stefan

    2009-05-25

    Thin silicon solar cells suffer from low light absorption compared to their thick counterparts, especially in the near infra-red regime. In order to obtain high energy conversion efficiency in thin solar cells, an efficient light trapping scheme is required. In this paper, we theoretically demonstrate significant enhancement in efficiency of thin crystalline silicon solar cells by using photonic crystals as the light absorbing layer. In particular, a relative increase of 11.15% and 3.87% in the energy conversion efficiency compared to the optimized conventional design is achieved for 2 microm and 10 microm thicknesses, respectively.

  11. High efficiency, low cost buried contact silicon solar cells

    SciTech Connect

    Honsberg, C.B.; Wenham, S.R.; Ebong, A.

    1994-12-31

    The buried contact (BC) technology has demonstrated both an efficiency and cost advantage over conventional screen printed solar cells. New BC structures, in particular the double sided (DS) BC cell, allow further improvements in cost and efficiency. Improvements in efficiency arise through improved rear surface passivation. Experimental results from DSBC cells using various passivation methods demonstrate that a floating junction (FJ) passivates as well as passivation schemes used with high efficiency cells. 2D analysis and experimental results both show localized defects have prevented FJ passivation from achieving its potential and that optimization of the rear doping or by bifacial operation can improve performance.

  12. Development of high-efficiency solar cells on silicon web

    NASA Technical Reports Server (NTRS)

    Rohatgi, A.; Meier, D. L.; Campbell, R. B.; Seidensticker, R. G.; Rai-Choudhury, P.

    1984-01-01

    The development of high efficiency solar cells on a silicon web is discussed. Heat treatment effects on web quality; the influence of twin plane lamellae, trace impurities and stress on minority carrier lifetime; and the fabrication of cells are discussed.

  13. III-V High-Efficiency Multijunction Photovoltaics (Fact Sheet)

    SciTech Connect

    Not Available

    2011-06-01

    Capabilities fact sheet that includes scope, core competencies and capabilities, and contact/web information for III-V High-Efficiency Multijunction Photovoltaics at the National Center for Photovoltaics.

  14. High efficiency solar cell research for space applications

    NASA Technical Reports Server (NTRS)

    Flood, D. J.

    1985-01-01

    A review is given of NASA photovoltaic research with emphasis on the activities of the Lewis Research Center. High efficiency solar cell research is discussed, as well as solar arrays, multi-junction cell bandgaps, and plasmon coupling.

  15. Recent developments in high-efficiency PV cells

    SciTech Connect

    Deb, S.

    2000-05-22

    Enormous progress has been made in recent years on a number of photovoltaic (PV) materials and devices in terms of conversion efficiencies. Ultrahigh-efficiency (>30{percent}) PV cells have been fabricated from gallium arsenide (GaAs) and its ternary alloys such as gallium indium phosphide (GaInP{sub 2}). The high-efficiency GaAs-based solar cells are being produced on a commercial scale, particularly for space applications. Efficiencies in the range of 18{percent} to 24{percent} have been achieved in traditional silicon-based devices fabricated from both multicrystalline and single-crystal materials. Major advances in efficiency have also been made on various thin-film solar cells based on amorphous silicon (aSi:H), copper gallium indium diselenide (CIGS), and cadmium telluride materials. This paper gives a brief overview of the recent progress in PV cell efficiencies based on these materials and devices.

  16. Global climate change: Mitigation opportunities high efficiency large chiller technology

    SciTech Connect

    Stanga, M.V.

    1997-12-31

    This paper, comprised of presentation viewgraphs, examines the impact of high efficiency large chiller technology on world electricity consumption and carbon dioxide emissions. Background data are summarized, and sample calculations are presented. Calculations show that presently available high energy efficiency chiller technology has the ability to substantially reduce energy consumption from large chillers. If this technology is widely implemented on a global basis, it could reduce carbon dioxide emissions by 65 million tons by 2010.

  17. High efficiency magneto-optical trap for unstable isotopes

    NASA Astrophysics Data System (ADS)

    Aubin, S.; Gomez, E.; Orozco, L. A.; Sprouse, G. D.

    2003-10-01

    We have trapped over 250 000 210Fr in a new on-line high efficiency magneto-optical trap (MOT). We describe the new apparatus and present an overview of high-efficiency MOTs for trapping rare isotopes. These traps depend on three critical components: a dry-film coating, a neutralizer, and the optical trap. We have developed a series of independent tests of the effectiveness of these components, and have used the results to construct our trap.

  18. Energy efficient engine: High pressure turbine uncooled rig technology report

    NASA Technical Reports Server (NTRS)

    Gardner, W. B.

    1979-01-01

    Results obtained from testing five performance builds (three vane cascades and two rotating rigs of the Energy Efficient Engine uncooled rig have established the uncooled aerodynamic efficiency of the high-pressure turbine at 91.1 percent. This efficiency level was attained by increasing the rim speed and annulus area (AN(2)), and by increasing the turbine reaction level. The increase in AN(2) resulted in a performance improvement of 1.15 percent. At the design point pressure ratio, the increased reaction level rig demonstrated an efficiency of 91.1 percent. The results of this program have verified the aerodynamic design assumptions established for the Energy Efficient Engine high-pressure turbine component.

  19. Production technology for high efficiency ion implanted solar cells

    NASA Technical Reports Server (NTRS)

    Kirkpatrick, A. R.; Minnucci, J. A.; Greenwald, A. C.; Josephs, R. H.

    1978-01-01

    Ion implantation is being developed for high volume automated production of silicon solar cells. An implanter designed for solar cell processing and able to properly implant up to 300 4-inch wafers per hour is now operational. A machine to implant 180 sq m/hr of solar cell material has been designed. Implanted silicon solar cells with efficiencies exceeding 16% AM1 are now being produced and higher efficiencies are expected. Ion implantation and transient processing by pulsed electron beams are being integrated with electrostatic bonding to accomplish a simple method for large scale, low cost production of high efficiency solar cell arrays.

  20. Phenomena and Performance of High-Efficiency Split Spectrum Photovoltaics

    NASA Astrophysics Data System (ADS)

    Downs, Chandler

    High-efficiency photovoltaics are one of the most promising technologies for supplying sustainable energy in the near future. These technologies allow for high energy conversion efficiencies and long system lifetimes, which is becoming an increasingly profitable power generation option. One high-efficiency photovoltaic technology gaining increasing attention recent years is that of split-spectrum photovoltaics. This technology divides the incident solar spectrum on the basis of wavelength, directing each portion of the spectrum to a different cell where the light can be utilized most efficiently. In this dissertation, a number of aspects of high-efficiency photovoltaics, most notably split-spectrum photovoltaics, are examined. First, the ideal bandgap placements of the subcells of a split-spectrum photovoltaic system are calculated, specifically determined with an eye towards practical fabrication of the cells. Two viable designs are determined which improve theoretical absolute conversion efficiency by 4-5%. Next, those systems are simulated using the TCAD Sentaurus software package to project conversion efficiencies and determine additional device specifications (doping levels, layer thicknesses, etc.). These cells show comparable conversion efficiencies to high performing, full-spectrum multijunction photovoltaics in fabrication today. In the last section, a theoretical examination of semiconductor performance under high optical concentration is performed, including the prediction and characterization of various phenomena in those devices. This work aims to improve the understanding of the performance of high concentration photovoltaics, most notably split-spectrum photovoltaics. This understanding will aid in the advancement of this technology as a widespread, sustainable energy source for use worldwide, reducing greenhouse emissions and providing cheap, clean energy.

  1. Highly efficient carrier multiplication in PbS nanosheets

    PubMed Central

    Aerts, Michiel; Bielewicz, Thomas; Klinke, Christian; Grozema, Ferdinand C.; Houtepen, Arjan J.; Schins, Juleon M.; Siebbeles, Laurens D. A.

    2014-01-01

    Semiconductor nanocrystals are promising for use in cheap and highly efficient solar cells. A high efficiency can be achieved by carrier multiplication (CM), which yields multiple electron-hole pairs for a single absorbed photon. Lead chalcogenide nanocrystals are of specific interest, since their band gap can be tuned to be optimal to exploit CM in solar cells. Interestingly, for a given photon energy CM is more efficient in bulk PbS and PbSe, which has been attributed to the higher density of states. Unfortunately, these bulk materials are not useful for solar cells due to their low band gap. Here we demonstrate that two-dimensional PbS nanosheets combine the band gap of a confined system with the high CM efficiency of bulk. Interestingly, in thin PbS nanosheets virtually the entire excess photon energy above the CM threshold is used for CM, in contrast to quantum dots, nanorods and bulk lead chalcogenide materials. PMID:24781188

  2. Interactions between the HIV-TAT transduction domain and cell membranes

    NASA Astrophysics Data System (ADS)

    Mishra, Abhijit; Wong, Gerard

    2006-03-01

    Biologically active molecules such as proteins and oligonucleotides can be transduced into cells with high efficiency when covalently linked to a Protein Transduction Domain (PTD), such as the TAT domain in the HIV virus. All PTDs have a high content of basic amino acids resulting in a net positive charge. Electrostatic interactions between cationic PTDs and the negatively charged phospholipids that constitute the plasma membrane are likely to be responsible for peptide uptake, but no detailed structural studies exist. We compare membrane structures induced by the cationic TAT domain and those induced by other cationic polypeptides as a function of membrane composition using synchrotron x-ray scattering, and examine possible mechanisms of the anomalous transduction.

  3. Recombinant TAT-Gelonin Fusion Toxin: Synthesis and Characterization of Heparin/Protamine-Regulated Cell Transduction

    PubMed Central

    Zhang, Jian; Huang, Yongzhuo; He, Huining; Wang, Mei; Min, Kyoung Ah; Yang, Victor C.

    2014-01-01

    Protein toxins, such as gelonin, are highly desirable anti-cancer drug candidates due to their unparalleled potency and repetitive reaction mechanism in inhibiting protein translation. However, for its potential application in cancer therapy, there remains the cell membrane barrier that allows permeation of only small molecules, which must be overcome. To address this challenge, we conjugated gelonin with a protein transduction domain (PTD), the TAT peptide, via genetic recombination. The chimeric TAT-gelonin fusion protein (TAT-Gel) retained equipotent N-glycosidase activity yet displayed greater cell uptake than unmodified recombinant gelonin (rGel), thereby yielding a significantly augmented cytotoxic activity. Remarkably, TATGel displayed up to 177-fold lower IC50 (avg. 54.3 nM) than rGel (avg. IC50: 3640 nM) in tested cell lines. This enhanced cytotoxicity, however, also raised potential toxicity concerns due to the non-selectivity of PTD in its mediated cell transduction. To solve this problem, we investigated the plausibility of regulating the cell transduction of TAT-Gel via a reversible masking using heparin and protamine. Here, we demonstrated, both in vitro and in vivo, that the cell transduction of TAT-Gel can be completely curbed with heparin and yet this heparin block can be efficiently reversed by the addition of protamine. This reversible tight regulation of the cell transduction of TAT-Gel by heparin and protamine sheds light of possible application of TATGel in achieving a highly effective yet safe drug therapy for the treatment of tumors. PMID:24733757

  4. Research on stable, high-efficiency, amorphous silicon multijunction modules

    SciTech Connect

    Catalano, A.; Bennett, M.; Chen, L.; D'Aiello, R.; Fieselmann, B.; Li, Y.; Newton, J.; Podlesny, R.; Yang, L. . Thin Film Div.)

    1992-08-01

    This report describes work to demonstrate a multijunction module with a stabilized'' efficiency (600 h, 50{degrees}C, AM1.5) of 10.5%. Triple-junction devices and modules using a-Si:H alloys with carbon and germanium were developed to meet program goals. ZnO was used to provide a high optical transmission front contact. Proof of concept was obtained for several important advances deemed to be important for obtaining high (12.5%) stabilized efficiency. They were (1) stable, high-quality a-SiC:H devices and (2) high-transmission, textured ZnO. Although these developments were not scaled up and included in modules, triple-junction module efficiencies as high as 10.85% were demonstrated. NREL measured 9.62% and 9.00% indoors and outdoors, respectively. The modules are expected to lose no more than 20% of their initial performance. 28 refs.

  5. High surface area electrode for high efficient microbial electrosynthesis

    NASA Astrophysics Data System (ADS)

    Nie, Huarong; Cui, Mengmeng; Lu, Haiyun; Zhang, Tian; Russell, Thomas; Lovley, Derek

    2012-02-01

    Microbial electrosynthesis, a process in which microorganisms directly accept electrons from an electrode to convert carbon dioxide and water into multi carbon organic compounds, affords a novel route for the generation of valuable products from electricity or even wastewater. The surface area of the electrode is critical for high production. A biocompatible, highly conductive, three-dimensional cathode was fabricated from a carbon nanotube textile composite to support the microorganism to produce acetate from carbon dioxide. The high surface area and macroscale porous structure of the intertwined CNT coated textile ?bers provides easy microbe access. The production of acetate using this cathode is 5 fold larger than that using a planar graphite electrode with the same volume. Nickel-nanowire-modified carbon electrodes, fabricated by microwave welding, increased the surface area greatly, were able to absorb more bacteria and showed a 1.5 fold increase in performance

  6. Process and design considerations for high-efficiency solar cells

    NASA Technical Reports Server (NTRS)

    Rohati, A.; Rai-Choudhury, P.

    1985-01-01

    This paper shows that oxide surface passivation coupled with optimum multilayer anti-reflective coating can provide approx. 3% (absolute) improvement in solar cell efficiency. Use of single-layer AR coating, without passivation, gives cell efficiencies in the range of 15 to 15.5% on high-quality, 4 ohm-cm as well as 0.1 to 0.2 ohm-cm float-zone silicon. Oxide surface passivation alone raises the cell efficiency to or = 17%. An optimum double-layer AR coating on oxide-passivated cells provides an additional approx. 5 to 10% improvement over a single-layer AR-coated cell, resulting in cell efficiencies in excess of 18%. Experimentally observed improvements are supported by model calculations and an approach to or = 20% efficient cells is discussed.

  7. A highly efficient cocaine detoxifying enzyme obtained by computational design

    PubMed Central

    Zheng, Fang; Xue, Liu; Hou, Shurong; Liu, Junjun; Zhan, Max; Yang, Wenchao; Zhan, Chang-Guo

    2014-01-01

    Compared to naturally occurring enzymes, computationally designed enzymes are usually much less efficient, with their catalytic activities being more than six orders of magnitude below the diffusion limit. Here we use a two-step computational design approach, combined with experimental work, to design a highly efficient cocaine hydrolising enzyme. We engineer E30-6 from human butyrylcholinesterase (BChE), which is specific for cocaine hydrolysis, and obtain a much higher catalytic efficiency for cocaine conversion than for conversion of the natural BChE substrate, acetylcholine (ACh). The catalytic efficiency of E30-6 for cocaine hydrolysis is comparable to that of the most efficient known naturally-occurring hydrolytic enzyme, acetylcholinesterase, the catalytic activity of which approaches the diffusion limit. We further show that E30-6 can protect mice from a subsequently administered lethal dose of cocaine, suggesting the enzyme may have therapeutic potential in the setting of cocaine detoxification or cocaine abuse. PMID:24643289

  8. High Efficiency Adsorption Chillers: High Efficiency Adsorption Cooling Using Metal Organic Heat Carriers

    SciTech Connect

    2010-10-01

    BEETIT Project: PNNL is incorporating significant improvements in materials that adsorb liquids or gases to design more efficient adsorption chillers. An adsorption chiller is a type of air conditioner that is powered by heat, solar or waste heat, or combustion of natural gas. Unlike typical chillers, this type has few moving parts and uses almost no electricity to operate. PNNL is designing adsorbent materials at the molecular level with at least 3 times higher refrigerant capacity and up to 20 times faster kinetics than adsorbents used in current chillers. By using the new adsorbent, PNNL is able to create a chiller that is significantly smaller, has twice the energy efficiency, and lower costs for materials and assembly time compared to conventional adsorption chillers.

  9. Development of high-efficiency solar cells on silicon web

    NASA Technical Reports Server (NTRS)

    Meier, D. L.; Greggi, J.; Okeeffe, T. W.; Rai-Choudhury, P.

    1986-01-01

    Work was performed to improve web base material with a goal of obtaining solar cell efficiencies in excess of 18% (AM1). Efforts in this program are directed toward identifying carrier loss mechanisms in web silicon, eliminating or reducing these mechanisms, designing a high efficiency cell structure with the aid of numerical models, and fabricating high efficiency web solar cells. Fabrication techniques must preserve or enhance carrier lifetime in the bulk of the cell and minimize recombination of carriers at the external surfaces. Three completed cells were viewed by cross-sectional transmission electron microscopy (TEM) in order to investigate further the relation between structural defects and electrical performance of web cells. Consistent with past TEM examinations, the cell with the highest efficiency (15.0%) had no dislocations but did have 11 twin planes.

  10. Highly-efficient THz generation using nonlinear plasmonic metasurfaces

    NASA Astrophysics Data System (ADS)

    Tymchenko, M.; Gomez-Diaz, J. S.; Lee, J.; Belkin, M. A.; Alù, A.

    2017-10-01

    Nonlinear metasurfaces loaded with multi-quantum-well (MQW) heterostructures constitute a rapidly progressing class of optical devices that combine high nonlinear generation efficiency with an ultrathin profile. Here, we introduce and discuss terahertz (THz) difference-frequency generation (DFG) using MQW-based plasmonic metasurfaces and present a comprehensive theory for their rigorous electromagnetic analysis. We explicitly take into account complex phenomena associated with the local intensity saturation of intersubband transitions and identify fundamental upper-bounds for DFG conversion efficiency. Using this framework, we design and analyze a nonlinear DFG metasurface providing giant DFG nonlinear response and conversion efficiency up to 0.01% at 5.8 THz. Such metasurface can be used to generate 0.15 mW of THz power using pump intensities in the kW cm-2 range. We envision that such DFG metasurfaces can become a platform for uncooled, compact, and highly-efficient continuous-wave THz sources.

  11. Efficient Unsteady Flow Visualization with High-Order Access Dependencies

    SciTech Connect

    Zhang, Jiang; Guo, Hanqi; Yuan, Xiaoru

    2016-04-19

    We present a novel high-order access dependencies based model for efficient pathline computation in unsteady flow visualization. By taking longer access sequences into account to model more sophisticated data access patterns in particle tracing, our method greatly improves the accuracy and reliability in data access prediction. In our work, high-order access dependencies are calculated by tracing uniformly-seeded pathlines in both forward and backward directions in a preprocessing stage. The effectiveness of our proposed approach is demonstrated through a parallel particle tracing framework with high-order data prefetching. Results show that our method achieves higher data locality and hence improves the efficiency of pathline computation.

  12. High efficiency tantalum-based ceramic composite structures

    NASA Technical Reports Server (NTRS)

    Stewart, David A. (Inventor); Leiser, Daniel B. (Inventor); DiFiore, Robert R. (Inventor); Katvala, Victor W. (Inventor)

    2010-01-01

    Tantalum-based ceramics are suitable for use in thermal protection systems. These composite structures have high efficiency surfaces (low catalytic efficiency and high emittance), thereby reducing heat flux to a spacecraft during planetary re-entry. These ceramics contain tantalum disilicide, molybdenum disilicide and borosilicate glass. The components are milled, along with a processing aid, then applied to a surface of a porous substrate, such as a fibrous silica or carbon substrate. Following application, the coating is then sintered on the substrate. The composite structure is substantially impervious to hot gas penetration and capable of surviving high heat fluxes at temperatures approaching 3000.degree. F. and above.

  13. Metal–Dielectric Waveguides for High Efficiency Fluorescence Imaging

    PubMed Central

    Zhu, Liangfu; Zhang, Douguo; Wang, Ruxue; Wang, Pei; Ming, Hai; Badugu, Ramachandram; Du, Luping; Yuan, Xiaocong; Lakowicz, Joseph R.

    2015-01-01

    We demonstrate that Metal–Dielectric Waveguide structures (MDWs) with high efficiency of fluorescence coupling can be suitable as substrates for fluorescence imaging. This hybrid MDWs consists of a continuous metal film and a dielectric top layer. The optical modes sustaining inside this structure can be excited with a high numerical aperture (N.A) objective, and then focused into a virtual optical probe with high intensity, leading to efficient excitation of fluorophores deposited on top of the MDWs. The emitted fluorophores couple with the optical modes thus enabling the directional emission, which is verified by the back focal plane (BFP) imaging. These unique properties of MDWs have been adopted in a scanning laser confocal optical microscopy, and show the merit of high efficiency fluorescence imaging. MDWs can be easily fabricated by vapor deposition and/or spin coating, the silica surface of the MDWs is suitable for biomolecule tethering, and will offer new opportunities for cell biology and biophysics research. PMID:26525494

  14. High-Efficiency Solar Cells on Low-Cost Substrates

    NASA Technical Reports Server (NTRS)

    Daiello, R. V.; Robinson, P. H.

    1982-01-01

    High-efficiency solar cells made in thin epitaxial films grown on low-cost commercial silicon substrates. Cost of cells is much less than if high-quality single-crystal silicon were used for substrates and performance of cells is almost as good.

  15. Clamp provides efficient connection for high-density currents

    NASA Technical Reports Server (NTRS)

    Mc Carthy, J. R.; Trebes, D. M.

    1967-01-01

    Electrical connector clamp /bus bar/ gives high contact-surface efficiency for providing a high current to thin wall stainless steel tubing containing hydrogen gas. It uses lead solder film to provide the electrical equivalent of a fusion bond without degrading the grain structure, permitting disassembly and reuse of the components.

  16. High efficiency thin-film GaAs solar cells

    SciTech Connect

    Zwerdling, S.; Wang, K.L.; Yeh, Y.C.M.

    1981-01-01

    This research is directed toward demonstrating the feasibility of producing high-efficiency GaAs solar cells with high power-to-weight ratio by organo-metallic chemical vapor deposition (OM-CVD) growth of thin epi-layers on suitable substrates. 4 refs.

  17. High Efficiency Pb-In Binary Metal Perovskite Solar Cells.

    PubMed

    Wang, Zhao-Kui; Li, Meng; Yang, Ying-Guo; Hu, Yun; Ma, Heng; Gao, Xing-Yu; Liao, Liang-Sheng

    2016-08-01

    Mixed Pb-In perovskite solar cells are fabricated by using lead(II) chloride and indium(III) chloride with methylammonium iodide. A maximum power conversion efficiency as high as 17.55% is achieved owing to the high quality of perovskites with multiple ordered crystal orientations. © 2016 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

  18. High-efficiency beam bending using graded photonic crystals.

    PubMed

    Oner, B B; Turduev, M; Kurt, H

    2013-05-15

    We explore beam-bending properties of graded index (GRIN) waveguide with hyperbolic secant profile. The transmission efficiency and bandwidth features are extracted for GRIN photonic crystal (PC) media composed of dielectric rods. Light guiding performance of the GRIN PC medium is analyzed for 90° and 180° waveguide bends. The finite-difference time-domain method is deployed to investigate the performance of the designed GRIN waveguides. By the help of proposed photonic configuration, bending of light is achieved with a high efficiency within a broad bandwidth, which promotes the use of GRIN PC structures for efficient light-bending purposes.

  19. Development of an Improved High Efficiency Thin Silicon Solar Cell

    NASA Technical Reports Server (NTRS)

    Storti, G.; Wrigley, C.

    1979-01-01

    Breakage and front contact failure in high efficiency, textured ultrathin cells was reduced as a consequence of the introduction of process modifications. In a small production run, over one hundred ultrathin cells, having an average AMO efficiency of 13%, were fabricated from 10-25 ohm cm silicon. An in-house aluminum paste for back surface field formation was developed that resulted in cell efficiencies equivalent to those from commercial pastes. The quality of the back surface field was found to be dependent on the orientation of the silicon slice during alloying.

  20. Investigation of high efficiency GaAs solar cells

    NASA Technical Reports Server (NTRS)

    Olsen, Larry C.; Dunham, Glen; Addis, F. W.; Huber, Dan; Linden, Kurt

    1989-01-01

    Investigations of basic mechanisms which limit the performance of high efficiency GaAs solar cells are discussed. P/N heteroface structures have been fabricated from MOCVD epiwafers. Typical AM1 efficiencies are in the 21 to 22 percent range, with a SERI measurement for one cell being 21.5 percent. The cells are nominally 1.5 x 1.5 cm in size. Studies have involved photoresponse, T-I-V analyses, and interpretation of data in terms of appropriate models to determine key cell parameters. Results of these studies are utilized to determine future approaches for increasing GaAs solar cell efficiencies.

  1. High efficiency upconversion nanophosphors for high-contrast bioimaging

    NASA Astrophysics Data System (ADS)

    Alkahtani, Masfer H.; Alghannam, Fahad S.; Sanchez, Carlos; Gomes, Carmen L.; Liang, Hong; Hemmer, Philip R.

    2016-12-01

    Upconversion nanoparticles (UCNPs) are of interest because they allow suppression of tissue autofluorescence and are therefore visible deep inside biological tissue. Compared to upconversion dyes, UCNPs have a lower pump intensity threshold, better photostability, and less toxicity. Recently, YVO4: Er+3, Yb+3 nanoparticles were shown to exhibit strong up-conversion luminescence with a relatively low 10 kW cm-2 excitation intensity even in water, which makes them excellent bio-imaging candidates. Herein, we investigate their use as internal probes in insects by injecting YVO4 : Er+3, Yb+3 nanoparticles into fire ants as a biological model, and obtain 2D optical images with 980 nm illumination. High-contrast images with high signal-to-noise ratio are observed by detecting the up-conversion fluorescence as the excitation laser is scanned.

  2. High efficiency upconversion nanophosphors for high-contrast bioimaging.

    PubMed

    Alkahtani, Masfer H; Alghannam, Fahad S; Sanchez, Carlos; Gomes, Carmen L; Liang, Hong; Hemmer, Philip R

    2016-12-02

    Upconversion nanoparticles (UCNPs) are of interest because they allow suppression of tissue autofluorescence and are therefore visible deep inside biological tissue. Compared to upconversion dyes, UCNPs have a lower pump intensity threshold, better photostability, and less toxicity. Recently, YVO4: Er(+3), Yb(+3) nanoparticles were shown to exhibit strong up-conversion luminescence with a relatively low 10 kW cm(-2) excitation intensity even in water, which makes them excellent bio-imaging candidates. Herein, we investigate their use as internal probes in insects by injecting YVO4 : Er(+3), Yb(+3) nanoparticles into fire ants as a biological model, and obtain 2D optical images with 980 nm illumination. High-contrast images with high signal-to-noise ratio are observed by detecting the up-conversion fluorescence as the excitation laser is scanned.

  3. High efficiency cw laser-pumped tunable alexandrite laser

    SciTech Connect

    Lai, S.T.; Shand, M.L.

    1983-10-01

    High efficiency cw alexandrite laser operation has been achieved. With longitudinal pumping by a krypton laser in a nearly concentric cavity, a 51% output power slope efficiency has been measured. Including the transmission at the input coupler mirror, a quantum yield of 85% has been attained above threshold. Tunability from 726 to 802 nm has also been demonstrated. The low loss and good thermal properties make alexandrite ideal for cw laser operation.

  4. High Power Klystrons for Efficient Reliable High Power Amplifiers.

    DTIC Science & Technology

    1980-11-01

    components of the space charge waves in the electron beam of a microwave tube are combined to produce more highly concentrated electron bunches raising the...the drift lengths to enhance the 2nd harmonic component in the space charge waves . The latter method was utilized in the VKC-7790. Computer...photographs, operating/ maintanance instructions, layout drawings/schematics and the purchase specification are included, in the above order, in

  5. High Efficiency Solar Power via Separated Photo and Voltaic Pathways

    SciTech Connect

    Michael J. Naughton

    2009-02-17

    This project demonstrates a novel nanostructured solar cell architecture capable of achieving high efficiency levels that is relatively simple and inexpensive to manufacture. The high efficiency will be achieved by the novel structure that separates the path of the photons from the path of the generated charge carriers. In this way, the photon path can be long for maximum light absorption, while the path for carriers can be short for maximum electronic energy harvesting. The combination of maximum light absorption coupled with maximum carrier harvesting is the basis for the expected high efficiency. The project will develop high efficiency solar cell prototypes utilizing this unique nanostructured architecture. The project addresses the fundamental limitation inherent in all current solar cell designs, and which opens a pathway to development for high efficiency solar cells at low cost. Realizing this goal will result in a levelized cost of electricity in the range of 10¢/kWh, which would achieve the long-sought goal of making photovoltaic electricity cost competitive with fossil-fuel generated electricity without any governmental subsidies. This breakthrough would spur the already rapid growth in the photovoltaic industry to an explosive pace, with significant, widespread benefit to the national economy and the nation’s energy security. The initial target of the program is to develop single-junction solar cells using ultrathin amorphous silicon with the performance approaching that of single crystal silicon cells.

  6. Multiple Exciton Generation for Highly Efficient Solar Cells

    NASA Astrophysics Data System (ADS)

    Nozik, Arthur

    2007-03-01

    In order to utilize solar power for the production of electricity and fuel on a massive scale, it will be necessary to develop solar photon conversion systems that have an appropriate combination of high efficiency and low capital cost (/m^2). One new potential approach to high solar cell efficiency is to utilize the unique properties of semiconductor quantum dot nanostructures to control the relaxation dynamics of photogenerated carriers to produce either enhanced photocurrent through efficient multiple exciton generation (MEG) or enhanced photopotential through hot electron transport and transfer processes. To achieve these desirable effects it is necessary to understand and control the dynamics of electron relaxation, cooling, multiple exciton generation , transport, and interfacial electron transfer of the photogenerated carriers with fs to ns time resolution. We have been studying these fundamental dynamics in bulk and nanoscale semiconductors (quantum dots, quantum wires, and quantum wells) using femtosecond transient absorption, photoluminescence, and THz spectroscopy. This work will be summarized and recent advances in creating multiple excitons from a single photon will be discussed, including a unique model to explain efficient MEG based on the coherent superposition of multiple excitonic states. Various possible configurations for quantum dot solar cells that could produce ultra-high conversion efficiencies for the production of electricity, as well as for producing solar fuels (for example, hydrogen from water splitting), will be discussed, along with associated thermodynamic calculations that show the increase in the maximum theoretical gain in solar photon conversion efficiency for both electricity and fuel production.

  7. Molecular basis of mechanosensory transduction

    NASA Astrophysics Data System (ADS)

    Gillespie, Peter G.; Walker, Richard G.

    2001-09-01

    Mechanotransduction - a cell's conversion of a mechanical stimulus into an electrical signal - reveals vital features of an organism's environment. From hair cells and skin mechanoreceptors in vertebrates, to bristle receptors in flies and touch receptors in worms, mechanically sensitive cells are essential in the life of an organism. The scarcity of these cells and the uniqueness of their transduction mechanisms have conspired to slow molecular characterization of the ensembles that carry out mechanotransduction. But recent progress in both invertebrates and vertebrates is beginning to reveal the identities of proteins essential for transduction.

  8. Highly Active Microbial Phosphoantigen Induces Rapid yet Sustained MEK/Erk- and PI-3K/Akt-Mediated Signal Transduction in Anti-Tumor Human γδ T-Cells

    PubMed Central

    Cardoso, Bruno A.; Lança, Telma; Grosso, Ana R.; deBarros, Ana; Martins, Leila R.; Barata, João T.; Silva-Santos, Bruno

    2009-01-01

    Background The unique responsiveness of Vγ9Vδ2 T-cells, the major γδ subset of human peripheral blood, to non-peptidic prenyl pyrophosphate antigens constitutes the basis of current γδ T-cell-based cancer immunotherapy strategies. However, the molecular mechanisms responsible for phosphoantigen-mediated activation of human γδ T-cells remain unclear. In particular, previous reports have described a very slow kinetics of activation of T-cell receptor (TCR)-associated signal transduction pathways by isopentenyl pyrophosphate and bromohydrin pyrophosphate, seemingly incompatible with direct binding of these antigens to the Vγ9Vδ2 TCR. Here we have studied the most potent natural phosphoantigen yet identified, (E)-4-hydroxy-3-methyl-but-2-enyl pyrophosphate (HMB-PP), produced by Eubacteria and Protozoa, and examined its γδ T-cell activation and anti-tumor properties. Methodology/Principal Findings We have performed a comparative study between HMB-PP and the anti-CD3ε monoclonal antibody OKT3, used as a reference inducer of bona fide TCR signaling, and followed multiple cellular and molecular γδ T-cell activation events. We show that HMB-PP activates MEK/Erk and PI-3K/Akt pathways as rapidly as OKT3, and induces an almost identical transcriptional profile in Vγ9+ T-cells. Moreover, MEK/Erk and PI-3K/Akt activities are indispensable for the cellular effects of HMB-PP, including γδ T-cell activation, proliferation and anti-tumor cytotoxicity, which are also abolished upon antibody blockade of the Vγ9+ TCR Surprisingly, HMB-PP treatment does not induce down-modulation of surface TCR levels, and thereby sustains γδ T-cell activation upon re-stimulation. This ultimately translates in potent human γδ T-cell anti-tumor function both in vitro and in vivo upon transplantation of human leukemia cells into lymphopenic mice, Conclusions/Significance The development of efficient cancer immunotherapy strategies critically depends on our capacity to maximize anti

  9. Aptamer modification improves the adenoviral transduction of malignant glioma cells.

    PubMed

    Chen, Hao; Zheng, Xiaojing; Di, BingYan; Wang, Dongyang; Zhang, Yaling; Xia, Haibin; Mao, Qinwen

    2013-12-01

    Adenovirus has shown increasing promise in the gene-viral therapy for glioblastoma, a treatment strategy that relies on the delivery of viruses or transgenes into tumor cells. However, targeting of adenovirus to human glioblastoma remains a challenge due to the low expression level of coxsackie and adenovirus receptor (CAR) in glioma cells. Aptamers are small and highly structured single-stranded oligonucleotides that bind at high affinity to a target molecule, and are good candidates for targeted imaging and therapy. In this study, to construct an aptamer-modified Ad5, we first genetically modified the HVR5 of Ad hexon by biotin acceptor peptide (BAP), which would be metabolically biotinylated during production in HEK293 cells, and then attached the biotin labeled aptamer to the modified Ad through avidin–biotin binding. The aptamers used in this study includes AS1411 and GBI-10. The former is a DNA aptamer that can bind to nucleolin, a nuclear matrix protein found on the surface of cancer cells. The latter is a DNA aptamer that can recognize the extracellular matrix protein tenascin-C on the surface of human glioblastoma cells. To examine if aptamer-modification of the hexon protein could improve the adenoviral transduction efficiency, a glioblastoma cell line, U251, was transduced with aptamer-modified Ads. The transduction efficiency of AS1411- or GBI-10-modified Ad was approximately 4.1-fold or 5.2-fold higher than that of the control. The data indicated that aptamer modified adenovirus would be a useful tool for cancer gene therapy. Crown Copyright © 2013. Published by Elsevier B.V. All rights reserved.

  10. High Efficient Synthesis of Iron-based Superconductors

    NASA Astrophysics Data System (ADS)

    Fang, Ai-Hua; Huang, Fu-Qiang; Xie, Xiao-Ming; Jiang, Mian-Heng

    We have performed systematic investigations aimed at high efficient synthesis of the 1111 family iron-based superconductors. By using meta-stable reactive starting materials of LnAs and FeO, assisted by mechanical alloying and fast heating, high purity samples with Tconset greater than 50K can be made with sintering temperatures between 1433K-1073K, and sintering time from 20 min to 40 h. High purity phase with sintering temperature as low as 973K was demonstrated successfully although Tconset fall below 50K and weak grain boundary suppressed greatly the zero resistance temperature. Ultra fast microwave sintering brings the sintering time further down to 5 min. Samples prepared by the above high efficient methods typically posses submicron grain and very high upper critical field, indicating very high pinning power. Besides offering cost advantages, the developed methods may play important roles in the exploit of novel superconductors.

  11. Design and analysis of a high efficiency linear power amplifier

    NASA Astrophysics Data System (ADS)

    Liu, Sucheng; Zhou, Luowei; Liu, Xiaodong; Lu, Weiguo

    2011-10-01

    A high efficiency linear power amplifier is introduced based on the idea of Switch-Linear Hybrid (SLH) power conversion. The SLH power amplifier developed from the conventional class B power amplifier, while the class B configuration power unit in the SLH power amplifier is fed by a dynamic switching power supply, not the usual constant DC power supply. Thus, the efficiency of the class B configuration power unit in SLH power amplifier can be greatly improved. By combining linear power amplifier with switching power supply, the SLH power amplifier has synthetic performance of high fidelity, high efficiency and excellent dynamic characteristics. In this article, analysis of SLH power amplifier is performed, especially focusing on its linear power unit which is the core of SLH power amplifier. Design considerations are also presented parallel with the analysis. Both the theoretical analysis and experimental results verify the validity of SLH power amplifier.

  12. Enabling High Efficiency Nanoplasmonics with Novel Nanoantenna Architectures

    NASA Astrophysics Data System (ADS)

    Cohen, Moshik; Shavit, Reuven; Zalevsky, Zeev

    2015-12-01

    Surface plasmon polaritons (SPPs) are propagating excitations that arise from coupling of light with collective electron oscillations. Characterized by high field intensity and nanometric dimensions, SPPs fashion rapid expansion of interest from fundamental and applicative perspectives. However, high metallic losses at optical frequencies still make nanoplasmonics impractical when high absolute efficiency is paramount, with major challenge is efficient plasmon generation in deep nanoscale. Here we introduce the Plantenna, the first reported nanodevice with the potential of addressing these limitations utilizing novel plasmonic architecture. The Plantenna has simple 2D structure, ultracompact dimensions and is fabricated on Silicon chip for future CMOS integration. We design the Plantenna to feed channel (20 nm × 20 nm) nanoplasmonic waveguides, achieving 52% coupling efficiency with Plantenna dimensions of λ3/17,000. We theoretically and experimentally show that the Plantenna enormously outperforms dipole couplers, achieving 28 dB higher efficiency with broad polarization diversity and huge local field enhancement. Our findings confirm the Plantenna as enabling device for high efficiency plasmonic technologies such as quantum nanoplasmonics, molecular strong coupling and plasmon nanolasers.

  13. Enabling High Efficiency Nanoplasmonics with Novel Nanoantenna Architectures.

    PubMed

    Cohen, Moshik; Shavit, Reuven; Zalevsky, Zeev

    2015-12-01

    Surface plasmon polaritons (SPPs) are propagating excitations that arise from coupling of light with collective electron oscillations. Characterized by high field intensity and nanometric dimensions, SPPs fashion rapid expansion of interest from fundamental and applicative perspectives. However, high metallic losses at optical frequencies still make nanoplasmonics impractical when high absolute efficiency is paramount, with major challenge is efficient plasmon generation in deep nanoscale. Here we introduce the Plantenna, the first reported nanodevice with the potential of addressing these limitations utilizing novel plasmonic architecture. The Plantenna has simple 2D structure, ultracompact dimensions and is fabricated on Silicon chip for future CMOS integration. We design the Plantenna to feed channel (20 nm × 20 nm) nanoplasmonic waveguides, achieving 52% coupling efficiency with Plantenna dimensions of λ(3)/17,000. We theoretically and experimentally show that the Plantenna enormously outperforms dipole couplers, achieving 28 dB higher efficiency with broad polarization diversity and huge local field enhancement. Our findings confirm the Plantenna as enabling device for high efficiency plasmonic technologies such as quantum nanoplasmonics, molecular strong coupling and plasmon nanolasers.

  14. Enabling High Efficiency Nanoplasmonics with Novel Nanoantenna Architectures

    PubMed Central

    Cohen, Moshik; Shavit, Reuven; Zalevsky, Zeev

    2015-01-01

    Surface plasmon polaritons (SPPs) are propagating excitations that arise from coupling of light with collective electron oscillations. Characterized by high field intensity and nanometric dimensions, SPPs fashion rapid expansion of interest from fundamental and applicative perspectives. However, high metallic losses at optical frequencies still make nanoplasmonics impractical when high absolute efficiency is paramount, with major challenge is efficient plasmon generation in deep nanoscale. Here we introduce the Plantenna, the first reported nanodevice with the potential of addressing these limitations utilizing novel plasmonic architecture. The Plantenna has simple 2D structure, ultracompact dimensions and is fabricated on Silicon chip for future CMOS integration. We design the Plantenna to feed channel (20 nm × 20 nm) nanoplasmonic waveguides, achieving 52% coupling efficiency with Plantenna dimensions of λ3/17,000. We theoretically and experimentally show that the Plantenna enormously outperforms dipole couplers, achieving 28 dB higher efficiency with broad polarization diversity and huge local field enhancement. Our findings confirm the Plantenna as enabling device for high efficiency plasmonic technologies such as quantum nanoplasmonics, molecular strong coupling and plasmon nanolasers. PMID:26620270

  15. Heat transparent high intensity high efficiency solar cell

    NASA Technical Reports Server (NTRS)

    Evans, J. C., Jr. (Inventor)

    1982-01-01

    An improved solar cell design is described. A surface of each solar cell has a plurality of grooves. Each groove has a vertical face and a slanted face that is covered by a reflecting metal. Light rays are reflected from the slanted face through the vertical face where they traverse a photovoltaic junction. As the light rays travel to the slanted face of an adjacent groove, they again traverse the junction. The underside of the reflecting coating directs the light rays toward the opposite surface of solar cell as they traverse the junction again. When the light rays travel through the solar cell and reach the saw toothed grooves on the under side, the process of reflection and repeatedly traversing the junction again takes place. The light rays ultimately emerge from the solar cell. These solar cells are particularly useful at very high levels of insolation because the infrared or heat radiation passes through the cells without being appreciably absorbed to heat the cell.

  16. Thermophotonics for ultra-high efficiency visible LEDs

    NASA Astrophysics Data System (ADS)

    Ram, Rajeev J.

    2017-02-01

    The wall-plug efficiency of modern light-emitting diodes (LEDs) has far surpassed all other forms of lighting and is expected to improve further as the lifetime cost of a luminaire is today dominated by the cost of energy. The drive towards higher efficiency inevitably opens the question about the limits of future enhancement. Here, we investigate thermoelectric pumping as a means for improving efficiency in wide-bandgap GaN based LEDs. A forward biased diode can work as a heat pump, which pumps lattice heat into the electrons injected into the active region via the Peltier effect. We experimentally demonstrate a thermally enhanced 450 nm GaN LED, in which nearly fourfold light output power is achieved at 615 K (compared to 295 K room temperature operation), with virtually no reduction in the wall-plug efficiency at bias V < ℏω/q. This result suggests the possibility of removing bulky heat sinks in high power LED products. A review of recent high-efficiency GaN LEDs suggests that Peltier thermal pumping plays a more important role in a wide range of modern LED structures that previously thought - opening a path to even higher efficiencies and lower lifetime costs for future lighting.

  17. High efficiency silicon solar cell based on asymmetric nanowire

    PubMed Central

    Ko, Myung-Dong; Rim, Taiuk; Kim, Kihyun; Meyyappan, M.; Baek, Chang-Ki

    2015-01-01

    Improving the efficiency of solar cells through novel materials and devices is critical to realize the full potential of solar energy to meet the growing worldwide energy demands. We present here a highly efficient radial p-n junction silicon solar cell using an asymmetric nanowire structure with a shorter bottom core diameter than at the top. A maximum short circuit current density of 27.5 mA/cm2 and an efficiency of 7.53% were realized without anti-reflection coating. Changing the silicon nanowire (SiNW) structure from conventional symmetric to asymmetric nature improves the efficiency due to increased short circuit current density. From numerical simulation and measurement of the optical characteristics, the total reflection on the sidewalls is seen to increase the light trapping path and charge carrier generation in the radial junction of the asymmetric SiNW, yielding high external quantum efficiency and short circuit current density. The proposed asymmetric structure has great potential to effectively improve the efficiency of the SiNW solar cells. PMID:26152914

  18. Energy Efficient Engine high pressure turbine component test performance report

    NASA Technical Reports Server (NTRS)

    Timko, L. P.

    1984-01-01

    The high pressure turbine for the General Electric Energy Efficient Engine is a two stage design of moderate loading. Results of detailed system studies led to selection of this configuration as the most appropriate in meeting the efficiency goals of the component development program. To verify the design features of the high pressure turbine, a full scale warm air turbine test rig with cooling flows simulated was run. Prior to this testing, an annular cascade test was run to select vane unguided turn for the first stage nozzle. Results of this test showed that the base configuration exceeded the lower unguided turning configuration by 0.48 percent in vane kinetic energy efficiency. The air turbine test program, consisting of extensive mapping and cooling flow variation as well as design point evaluation, demonstrated a design point efficiency level of 90.0 percent based on the thermodynamic definition. In terms of General Electric cycle definition, this efficiency was 92.5 percent. Based on this test, it is concluded that efficiency goals for the Flight Propulsion System were met.

  19. High efficiency silicon solar cell based on asymmetric nanowire.

    PubMed

    Ko, Myung-Dong; Rim, Taiuk; Kim, Kihyun; Meyyappan, M; Baek, Chang-Ki

    2015-07-08

    Improving the efficiency of solar cells through novel materials and devices is critical to realize the full potential of solar energy to meet the growing worldwide energy demands. We present here a highly efficient radial p-n junction silicon solar cell using an asymmetric nanowire structure with a shorter bottom core diameter than at the top. A maximum short circuit current density of 27.5 mA/cm(2) and an efficiency of 7.53% were realized without anti-reflection coating. Changing the silicon nanowire (SiNW) structure from conventional symmetric to asymmetric nature improves the efficiency due to increased short circuit current density. From numerical simulation and measurement of the optical characteristics, the total reflection on the sidewalls is seen to increase the light trapping path and charge carrier generation in the radial junction of the asymmetric SiNW, yielding high external quantum efficiency and short circuit current density. The proposed asymmetric structure has great potential to effectively improve the efficiency of the SiNW solar cells.

  20. Tunable high-efficient pulsed NH3 terahertz lasers

    NASA Astrophysics Data System (ADS)

    Jiu, Zhi-Xian; Li, Qiang; Zuo, Du-Luo; Miao, Liang; Cheng, Zu-Hai

    2012-03-01

    Experimental studies on a tunable efficient high-efficient pulsed NH3 terahertz (THz) lasers pumped by TEA CO2 lasers are presented. When NH3 is pumped by the different lines with the CO2 lasers, the generation of different terahertz radiations is discussed. The lines of the CO2 lasers are 9R(08), 9P(20), 10R(14), 10R(08), and 10R(06). To improve THz laser energy and photon conversion efficiency, different higher power of the CO2 laser can effectively improve THz laser energy and photon conversion efficiency. When the 9P(20) CO2 lasers with 9.68 J and 4.12 J pump NH3, the corresponding energy conversion efficiencies are 0.28% and 0.19%, increasing by a factor of about 1.5. The generation of terahertz radiations with energy as high as 27.29 mJ and 7.73 mJ are obtained, respectively, increasing by a factor of about 3.5. Meanwhile, for 10R(14) line, the energy conversion efficiencies increase to 8.5 times and the energy of THz lasers increase to 32 times.

  1. Tunable high-efficient pulsed NH3 terahertz lasers

    NASA Astrophysics Data System (ADS)

    Jiu, Zhi-Xian; Li, Qiang; Zuo, Du-Luo; Miao, Liang; Cheng, Zu-Hai

    2011-11-01

    Experimental studies on a tunable efficient high-efficient pulsed NH3 terahertz (THz) lasers pumped by TEA CO2 lasers are presented. When NH3 is pumped by the different lines with the CO2 lasers, the generation of different terahertz radiations is discussed. The lines of the CO2 lasers are 9R(08), 9P(20), 10R(14), 10R(08), and 10R(06). To improve THz laser energy and photon conversion efficiency, different higher power of the CO2 laser can effectively improve THz laser energy and photon conversion efficiency. When the 9P(20) CO2 lasers with 9.68 J and 4.12 J pump NH3, the corresponding energy conversion efficiencies are 0.28% and 0.19%, increasing by a factor of about 1.5. The generation of terahertz radiations with energy as high as 27.29 mJ and 7.73 mJ are obtained, respectively, increasing by a factor of about 3.5. Meanwhile, for 10R(14) line, the energy conversion efficiencies increase to 8.5 times and the energy of THz lasers increase to 32 times.

  2. High efficiency III-nitride light-emitting diodes

    DOEpatents

    Crawford, Mary; Koleske, Daniel; Cho, Jaehee; Zhu, Di; Noemaun, Ahmed; Schubert, Martin F; Schubert, E. Fred

    2013-05-28

    Tailored doping of barrier layers enables balancing of the radiative recombination among the multiple-quantum-wells in III-Nitride light-emitting diodes. This tailored doping enables more symmetric carrier transport and uniform carrier distribution which help to reduce electron leakage and thus reduce the efficiency droop in high-power III-Nitride LEDs. Mitigation of the efficiency droop in III-Nitride LEDs may enable the pervasive market penetration of solid-state-lighting technologies in high-power lighting and illumination.

  3. Highly engineered biocatalysts for efficient small molecule pharmaceutical synthesis.

    PubMed

    Lalonde, Jim

    2016-12-01

    Technologies for the engineering of biocatalysts for efficient synthesis of pharmaceutical targets have advanced dramatically over the last few years. Integration of computational methods for structural modeling, combined with high through put methods for expression and screening of biocatalysts and algorithms for mining experimental data, have allowed the creation of highly engineered biocatalysts for the efficient synthesis of pharmaceuticals. Methods for the synthesis of chiral alcohols and amines have been particularly successful, along with the creation of non-natural activities for such desirable reactions as cyclopropanation and esterification. Copyright © 2016 Elsevier Ltd. All rights reserved.

  4. Mutual physiological genetic mechanism of plant high water use efficiency and nutrition use efficiency.

    PubMed

    Cao, Hong-Xing; Zhang, Zheng-Bin; Xu, Ping; Chu, Li-Ye; Shao, Hong-Bo; Lu, Zhao-Hua; Liu, Jun-Hong

    2007-05-15

    Water deficiency and lower fertilizer utilization efficiency are major constraints of productivity and yield stability. Improvements of crop water use efficiency (WUE) and nutrient use efficiency (NUE) is becoming an important objective in crop breeding. With the introduction of new physiological and biological approaches, we can better understand the mutual genetics mechanism of high use efficiency of water and nutrient. Much work has been done in past decades mainly including the interactions between different fertilizers and water influences on root characteristics and crop growth. Fertilizer quantity and form were regulated in order to improve crop WUE. The crop WUE and NUE shared the same increment tendency during evolution process; some genes associated with WUE and NUE have been precisely located and marked on the same chromosomes, some genes related to WUE and NUE have been cloned and transferred into wheat and rice and other plants, they can enhance water and nutrient use efficiency. The proteins transporting nutrient and water were identified such as some water channel proteins. The advance on the mechanism of higher water and nutrient use efficiency in crop was reviewed in this article, and it could provide some useful information for further research on WUE and NUE in crop.

  5. Low Cost, High Efficiency, High Pressure Hydrogen Storage

    SciTech Connect

    Mark Leavitt

    2010-03-31

    A technical and design evaluation was carried out to meet DOE hydrogen fuel targets for 2010. These targets consisted of a system gravimetric capacity of 2.0 kWh/kg, a system volumetric capacity of 1.5 kWh/L and a system cost of $4/kWh. In compressed hydrogen storage systems, the vast majority of the weight and volume is associated with the hydrogen storage tank. In order to meet gravimetric targets for compressed hydrogen tanks, 10,000 psi carbon resin composites were used to provide the high strength required as well as low weight. For the 10,000 psi tanks, carbon fiber is the largest portion of their cost. Quantum Technologies is a tier one hydrogen system supplier for automotive companies around the world. Over the course of the program Quantum focused on development of technology to allow the compressed hydrogen storage tank to meet DOE goals. At the start of the program in 2004 Quantum was supplying systems with a specific energy of 1.1-1.6 kWh/kg, a volumetric capacity of 1.3 kWh/L and a cost of $73/kWh. Based on the inequities between DOE targets and Quantum’s then current capabilities, focus was placed first on cost reduction and second on weight reduction. Both of these were to be accomplished without reduction of the fuel system’s performance or reliability. Three distinct areas were investigated; optimization of composite structures, development of “smart tanks” that could monitor health of tank thus allowing for lower design safety factor, and the development of “Cool Fuel” technology to allow higher density gas to be stored, thus allowing smaller/lower pressure tanks that would hold the required fuel supply. The second phase of the project deals with three additional distinct tasks focusing on composite structure optimization, liner optimization, and metal.

  6. High efficiency all-polymer tandem solar cells

    PubMed Central

    Yuan, Jianyu; Gu, Jinan; Shi, Guozheng; Sun, Jianxia; Wang, Hai-Qiao; Ma, Wanli

    2016-01-01

    In this work, we have reported for the first time an efficient all-polymer tandem cell using identical sub-cells based on P2F-DO:N2200. A high power conversion efficiency (PCE) of 6.70% was achieved, which is among the highest efficiencies for all polymer solar cells and 43% larger than the PCE of single junction cell. The largely improved device performance can be mainly attributed to the enhanced absorption of tandem cell. Meanwhile, the carrier collection in device remains efficient by optimizing the recombination layer and sub-cell film thickness. Thus tandem structure can become an easy approach to effectively boost the performance of current all polymer solar cells. PMID:27226354

  7. High extraction efficiency ultraviolet light-emitting diode

    DOEpatents

    Wierer, Jonathan; Montano, Ines; Allerman, Andrew A.

    2015-11-24

    Ultraviolet light-emitting diodes with tailored AlGaN quantum wells can achieve high extraction efficiency. For efficient bottom light extraction, parallel polarized light is preferred, because it propagates predominately perpendicular to the QW plane and into the typical and more efficient light escape cones. This is favored over perpendicular polarized light that propagates along the QW plane which requires multiple, lossy bounces before extraction. The thickness and carrier density of AlGaN QW layers have a strong influence on the valence subband structure, and the resulting optical polarization and light extraction of ultraviolet light-emitting diodes. At Al>0.3, thinner QW layers (<2.5 nm are preferred) result in light preferentially polarized parallel to the QW plane. Also, active regions consisting of six or more QWs, to reduce carrier density, and with thin barriers, to efficiently inject carriers in all the QWs, are preferred.

  8. High efficiency quantum dot light emitting diodes from positive aging.

    PubMed

    Acharya, Krishna P; Titov, Alexandre; Hyvonen, Jake; Wang, Chenggong; Tokarz, Jean; Holloway, Paul H

    2017-10-05

    Colloidal quantum dot-polymer hybrid light emitting diodes (QLEDs) that exhibit external quantum efficiencies >12% for all three primary colors (21% from green) have been demonstrated. These high efficiencies result in part from a positive aging effect reported here for the first time, where positive aging means the efficiency of the QLED increased with time. We have achieved 470 h operational life time (T90) at 2550 nits for red QLEDs. At longer times, negative aging phenomena lead to lower luminance and limit the lifetime of the QLEDs. It is concluded that we have reasonable control over the efficiency of QLEDs. The next challenge is to achieve lifetimes sufficiently long for all three primary colors for applications such as in television and illumination.

  9. A high-efficiency heat-pump fan

    NASA Astrophysics Data System (ADS)

    Wright, T.; Lackey, R. S.; Veyo, S. E.

    1983-06-01

    Efforts toward development of a high-efficiency outdoor air mover for an advanced electric heat pump are documented. The design goal was to halve the outdoor air-moving electrical power. The prototype air mover at 850 rpm delivers 3070 scfm through the prototype outdoor unit with a pressure drop through the coil of 0.09 inches of water and consumes 150 watts of electrical power. The overall air-moving efficiency is estimated at about 35 percent compared with 19 percent for the conventionally-applied heat pump fan. Although this air mover will cost twice as much as the conventional heat pump air mover, this premium cost should be recoverable in less than four years through energy savings. The sound rating (SFN) for this air mover is less than 20. Means for improving fan efficiency by 5 percentage points, motor efficiency by 2.5 points, and to further quiet the fan were identified.

  10. Experimental Realization of High-Efficiency Counterfactual Computation

    NASA Astrophysics Data System (ADS)

    Kong, Fei; Ju, Chenyong; Huang, Pu; Wang, Pengfei; Kong, Xi; Shi, Fazhan; Jiang, Liang; Du, Jiangfeng

    2015-08-01

    Counterfactual computation (CFC) exemplifies the fascinating quantum process by which the result of a computation may be learned without actually running the computer. In previous experimental studies, the counterfactual efficiency is limited to below 50%. Here we report an experimental realization of the generalized CFC protocol, in which the counterfactual efficiency can break the 50% limit and even approach unity in principle. The experiment is performed with the spins of a negatively charged nitrogen-vacancy color center in diamond. Taking advantage of the quantum Zeno effect, the computer can remain in the not-running subspace due to the frequent projection by the environment, while the computation result can be revealed by final detection. The counterfactual efficiency up to 85% has been demonstrated in our experiment, which opens the possibility of many exciting applications of CFC, such as high-efficiency quantum integration and imaging.

  11. A metamaterial electromagnetic energy rectifying surface with high harvesting efficiency

    NASA Astrophysics Data System (ADS)

    Duan, Xin; Chen, Xing; Zhou, Lin

    2016-12-01

    A novel metamaterial rectifying surface (MRS) for electromagnetic energy capture and rectification with high harvesting efficiency is presented. It is fabricated on a three-layer printed circuit board, which comprises an array of periodic metamaterial particles in the shape of mirrored split rings, a metal ground, and integrated rectifiers employing Schottky diodes. Perfect impedance matching is engineered at two interfaces, i.e. one between free space and the surface, and the other between the metamaterial particles and the rectifiers, which are connected through optimally positioned vias. Therefore, the incident electromagnetic power is captured with almost no reflection by the metamaterial particles, then channeled maximally to the rectifiers, and finally converted to direct current efficiently. Moreover, the rectifiers are behind the metal ground, avoiding the disturbance of high power incident electromagnetic waves. Such a MRS working at 2.45 GHz is designed, manufactured and measured, achieving a harvesting efficiency up to 66.9% under an incident power density of 5 mW/cm2, compared with a simulated efficiency of 72.9%. This high harvesting efficiency makes the proposed MRS an effective receiving device in practical microwave power transmission applications.

  12. High efficient solar tracker based on a simple shutter structure

    NASA Astrophysics Data System (ADS)

    Chen, Jin-Jia; Liu, Te-Shu; Huang, Kuang-Lung; Lin, Po-Chih

    2013-09-01

    In many photovoltaic (PV) or sunlight-illumination systems, solar trackers are always essential to obtain high energy/flux concentration efficiency, and that would lead to increase cost and extra power consumption due to the complex structure and heavy weight of the trackers. To decrease the cost while without sacrificing efficiency, a Fresnellens concentrator incorporated with a simple and cheap shutter, which consists of high reflective mirrors instead of conventional trackers, is proposed in this paper to provide solar tracking during the daytime. Thus, the time-variant and slant-incident sunlight rays can be redirected to vertically incident upon the surface of the Fresnel lens by appropriately arranging mirrors and swinging them to the proper slant angles with respect to the orientation of sunlight. The computer simulation results show that power concentration efficiency over 90%, as compared with the efficiency of directly normal incident sunlight, can be achieved with the mirror reflectance of 0.97 and for any solar incident angle within +/-75 degrees to the normal of the Fresnel lens. To verify the feasibility and performance of the concentrator with the proposed shutter, a sunlight illumination system based on this novel structure is demonstrated. Both computer simulation and practical measurement results for the prototype of the sunlight illumination system are also given to compare with. The results prove the simple and high efficient shutter applicable to general PV or sunlight-illumination systems for solar tracking.

  13. Evaluation of a high-efficiency, filter-bank system.

    PubMed

    Martin, Stephen B; Beamer, Bryan R; Moyer, Ernest S

    2006-04-01

    National Institute for Occupational Safety and Health (NIOSH) investigators evaluated filtration efficiencies at three U.S. Postal Service (USPS) facilities. Ventilation and filtration systems (VFSs) had been installed after the 2001 bioterrorist attacks when the USPS unknowingly processed letters laden with B. anthracis spores. The new VFS units included high-efficiency particulate air (HEPA) filters and were required by USPS contract specifications to provide an overall filtration efficiency of at least 99.97% for particles between 0.3 microm and 3.0 micro m. The USPS evaluation involved a modification of methodology used to test total filtration system efficiency in agricultural tractor cab enclosures. The modified sampling strategy not only proved effective for monitoring the total filtration system component of VFS performance but also distinguished between filtration systems performing to the high USPS performance criteria and those needing repair or replacement. The results clearly showed the importance of choosing a pair of optical particle counters that have been closely matched immediately prior to testing. The modified methodology is readily adaptable to any workplace wishing to evaluate air filtration systems, including high-efficiency systems.

  14. Bandgap Engineering in High-Efficiency Multijunction Concentrator Cells

    SciTech Connect

    King, R. R.; Sherif, R. A.; Kinsey, G. S.; Kurtz, S.; Fetzer, C. M.; Edmondson, K. M.; Law, D. C.; Cotal, H. L.; Krut, D. D.; Ermer, J. H.; Karam, N. H.

    2005-08-01

    This paper discusses semiconductor device research paths under investigation with the aim of reaching the milestone efficiency of 40%. A cost analysis shows that achieving very high cell efficiencies is crucial for the realization of cost-effective photovoltaics, because of the strongly leveraging effect of efficiency on module packaging and balance-of systems costs. Lattice-matched (LM) GaInP/ GaInAs/ Ge 3-junction cells have achieved the highest independently confirmed efficiency at 175 suns, 25?C, of 37.3% under the standard AM1.5D, low-AOD terrestrial spectrum. Lattice-mismatched, or metamorphic (MM), materials offer still higher potential efficiencies, if the crystal quality can be maintained. Theoretical efficiencies well over 50% are possible for a MM GaInP/ 1.17-eV GaInAs/ Ge 3-junction cell limited by radiative recombination at 500 suns. The bandgap - open circuit voltage offset, (Eg/q) - Voc, is used as a valuable theoretical and experimental tool to characterize multijunction cells with subcell bandgaps ranging from 0.7 to 2.1 eV. Experimental results are presented for prototype 6-junction cells employing an active {approx}1.1-eV dilute nitride GaInNAs subcell, with active-area efficiency greater than 23% and over 5.3 V open-circuit voltage under the 1-sun AM0 space spectrum. Such cell designs have theoretical efficiencies under the terrestrial spectrum at 500 suns concentration exceeding 55% efficiency, even for lattice-matched designs.

  15. De novo designed protein transduction domain mimics from simple synthetic polymers.

    PubMed

    Tezgel, A Özgül; Telfer, Janice C; Tew, Gregory N

    2011-08-08

    Protein transduction domains (PTDs) that readily transverse cellular membranes are of great interest and are attractive tools for the intracellular delivery of bioactive molecules. Learning to program synthetic polymers and oligomers with the appropriate chemical information to capture adequately the biological activity of proteins is critical to our improved understanding of how these natural molecules work. In addition, the versatility of these synthetic mimics provides the opportunity to discover analogs with superior properties compared with their native sequences. Here we report the first detailed structure-activity relationship of a new PTD family of polymers based on a completely abiotic backbone. The synthetic approach easily allows doubling the density of guanidine functional groups, which increases the transduction efficiency of the sequences. Cellular uptake studies on three different cell lines (HEK 293T, CHO, and Jurkat T cells) confirm that these synthetic analogs are highly efficient novel protein transduction domain mimics (PTDMs), which are more effective than TAT(49-57) and nonaarginine (R9) and also highlight the usefulness of polymer chemistry at the chemistry-biology interface.

  16. Realization of highly efficient hexagonal boron nitride neutron detectors

    SciTech Connect

    Maity, A.; Doan, T. C.; Li, J.; Lin, J. Y.; Jiang, H. X.

    2016-08-16

    Here, we report the achievement of highly efficient 10B enriched hexagonal boron nitride (h- 10BN) direct conversion neutron detectors. These detectors were realized from freestanding 4-in. diameter h- 10BN wafers 43 μm in thickness obtained from epitaxy growth and subsequent mechanical separation from sapphire substrates. Both sides of the film were subjected to ohmic contact deposition to form a simple vertical “photoconductor-type” detector. Transport measurements revealed excellent vertical transport properties including high electrical resistivity (>1013 Ω cm) and mobility-lifetime (μτ) products. A much larger μτ product for holes compared to that of electrons along the c-axis of h- BN was observed, implying that holes (electrons) behave like majority (minority) carriers in undoped h- BN. Exposure to thermal neutrons from a californium-252 (252Cf) source moderated by a high density polyethylene moderator reveals that 43 μm h- 10BN detectors possess 51.4% detection efficiency at a bias voltage of 400 V, which is the highest reported efficiency for any semiconductor-based neutron detector. The results point to the possibility of obtaining highly efficient, compact solid-state neutron detectors with high gamma rejection and low manufacturing and maintenance costs.

  17. Realization of highly efficient hexagonal boron nitride neutron detectors

    DOE PAGES

    Maity, A.; Doan, T. C.; Li, J.; ...

    2016-08-16

    Here, we report the achievement of highly efficient 10B enriched hexagonal boron nitride (h- 10BN) direct conversion neutron detectors. These detectors were realized from freestanding 4-in. diameter h- 10BN wafers 43 μm in thickness obtained from epitaxy growth and subsequent mechanical separation from sapphire substrates. Both sides of the film were subjected to ohmic contact deposition to form a simple vertical “photoconductor-type” detector. Transport measurements revealed excellent vertical transport properties including high electrical resistivity (>1013 Ω cm) and mobility-lifetime (μτ) products. A much larger μτ product for holes compared to that of electrons along the c-axis of h- BN wasmore » observed, implying that holes (electrons) behave like majority (minority) carriers in undoped h- BN. Exposure to thermal neutrons from a californium-252 (252Cf) source moderated by a high density polyethylene moderator reveals that 43 μm h- 10BN detectors possess 51.4% detection efficiency at a bias voltage of 400 V, which is the highest reported efficiency for any semiconductor-based neutron detector. The results point to the possibility of obtaining highly efficient, compact solid-state neutron detectors with high gamma rejection and low manufacturing and maintenance costs.« less

  18. Realization of highly efficient hexagonal boron nitride neutron detectors

    NASA Astrophysics Data System (ADS)

    Maity, A.; Doan, T. C.; Li, J.; Lin, J. Y.; Jiang, H. X.

    2016-08-01

    We report the achievement of highly efficient 10B enriched hexagonal boron nitride (h-10BN) direct conversion neutron detectors. These detectors were realized from freestanding 4-in. diameter h-10BN wafers 43 μm in thickness obtained from epitaxy growth and subsequent mechanical separation from sapphire substrates. Both sides of the film were subjected to ohmic contact deposition to form a simple vertical "photoconductor-type" detector. Transport measurements revealed excellent vertical transport properties including high electrical resistivity (>1013 Ω cm) and mobility-lifetime (μτ) products. A much larger μτ product for holes compared to that of electrons along the c-axis of h-BN was observed, implying that holes (electrons) behave like majority (minority) carriers in undoped h-BN. Exposure to thermal neutrons from a californium-252 (252Cf) source moderated by a high density polyethylene moderator reveals that 43 μm h-10BN detectors possess 51.4% detection efficiency at a bias voltage of 400 V, which is the highest reported efficiency for any semiconductor-based neutron detector. The results point to the possibility of obtaining highly efficient, compact solid-state neutron detectors with high gamma rejection and low manufacturing and maintenance costs.

  19. Realization of highly efficient hexagonal boron nitride neutron detectors

    SciTech Connect

    Maity, A.; Doan, T. C.; Li, J.; Lin, J. Y.; Jiang, H. X.

    2016-08-16

    Here, we report the achievement of highly efficient 10B enriched hexagonal boron nitride (h- 10BN) direct conversion neutron detectors. These detectors were realized from freestanding 4-in. diameter h- 10BN wafers 43 μm in thickness obtained from epitaxy growth and subsequent mechanical separation from sapphire substrates. Both sides of the film were subjected to ohmic contact deposition to form a simple vertical “photoconductor-type” detector. Transport measurements revealed excellent vertical transport properties including high electrical resistivity (>1013 Ω cm) and mobility-lifetime (μτ) products. A much larger μτ product for holes compared to that of electrons along the c-axis of h- BN was observed, implying that holes (electrons) behave like majority (minority) carriers in undoped h- BN. Exposure to thermal neutrons from a californium-252 (252Cf) source moderated by a high density polyethylene moderator reveals that 43 μm h- 10BN detectors possess 51.4% detection efficiency at a bias voltage of 400 V, which is the highest reported efficiency for any semiconductor-based neutron detector. The results point to the possibility of obtaining highly efficient, compact solid-state neutron detectors with high gamma rejection and low manufacturing and maintenance costs.

  20. Processing technology for high efficiency silicon solar cells

    NASA Technical Reports Server (NTRS)

    Spitzer, M. B.; Keavney, C. J.

    1985-01-01

    Recent advances in silicon solar cell processing have led to attainment of conversion efficiency approaching 20%. The basic cell design is investigated and features of greatest importance to achievement of 20% efficiency are indicated. Experiments to separately optimize high efficiency design features in test structures are discussed. The integration of these features in a high efficiency cell is examined. Ion implantation has been used to achieve optimal concentrations of emitter dopant and junction depth. The optimization reflects the trade-off between high sheet conductivity, necessary for high fill factor, and heavy doping effects, which must be minimized for high open circuit voltage. A second important aspect of the design experiments is the development of a passivation process to minimize front surface recombination velocity. The manner in which a thin SiO2 layer may be used for this purpose is indicated without increasing reflection losses, if the antireflection coating is properly designed. Details are presented of processing intended to reduce recombination at the contact/Si interface. Data on cell performance (including CZ and ribbon) and analysis of loss mechanisms are also presented.

  1. Meeting Report: Teaching Signal Transduction

    ERIC Educational Resources Information Center

    Kramer, IJsbrand; Thomas, Geraint

    2006-01-01

    In July, 2005, the European Institute of Chemistry and Biology at the campus of the University of Bordeaux, France, hosted a focused week of seminars, workshops, and discussions around the theme of "teaching signal transduction." The purpose of the summer school was to offer both junior and senior university instructors a chance to…

  2. Meeting Report: Teaching Signal Transduction

    ERIC Educational Resources Information Center

    Kramer, IJsbrand; Thomas, Geraint

    2006-01-01

    In July, 2005, the European Institute of Chemistry and Biology at the campus of the University of Bordeaux, France, hosted a focused week of seminars, workshops, and discussions around the theme of "teaching signal transduction." The purpose of the summer school was to offer both junior and senior university instructors a chance to…

  3. High power, high efficiency millimeter wavelength traveling wave tubes for high rate communications from deep space

    NASA Technical Reports Server (NTRS)

    Dayton, James A., Jr.

    1991-01-01

    The high-power transmitters needed for high data rate communications from deep space will require a new class of compact, high efficiency traveling wave tubes (TWT's). Many of the recent TWT developments in the microwave frequency range are generically applicable to mm wave devices, in particular much of the technology of computer aided design, cathodes, and multistage depressed collectors. However, because TWT dimensions scale approximately with wavelength, mm wave devices will be physically much smaller with inherently more stringent fabrication tolerances and sensitivity to thermal dissipation.

  4. High power, high efficiency millimeter wavelength traveling wave tubes for high rate communications from deep space

    NASA Technical Reports Server (NTRS)

    Dayton, James A., Jr.

    1991-01-01

    The high-power transmitters needed for high data rate communications from deep space will require a new class of compact, high efficiency traveling wave tubes (TWT's). Many of the recent TWT developments in the microwave frequency range are generically applicable to mm wave devices, in particular much of the technology of computer aided design, cathodes, and multistage depressed collectors. However, because TWT dimensions scale approximately with wavelength, mm wave devices will be physically much smaller with inherently more stringent fabrication tolerances and sensitivity to thermal dissipation.

  5. Highly efficient light management for perovskite solar cells.

    PubMed

    Wang, Dong-Lin; Cui, Hui-Juan; Hou, Guo-Jiao; Zhu, Zhen-Gang; Yan, Qing-Bo; Su, Gang

    2016-01-06

    Organic-inorganic halide perovskite solar cells have enormous potential to impact the existing photovoltaic industry. As realizing a higher conversion efficiency of the solar cell is still the most crucial task, a great number of schemes were proposed to minimize the carrier loss by optimizing the electrical properties of the perovskite solar cells. Here, we focus on another significant aspect that is to minimize the light loss by optimizing the light management to gain a high efficiency for perovskite solar cells. In our scheme, the slotted and inverted prism structured SiO2 layers are adopted to trap more light into the solar cells, and a better transparent conducting oxide layer is employed to reduce the parasitic absorption. For such an implementation, the efficiency and the serviceable angle of the perovskite solar cell can be promoted impressively. This proposal would shed new light on developing the high-performance perovskite solar cells.

  6. Highly efficient light management for perovskite solar cells

    PubMed Central

    Wang, Dong-Lin; Cui, Hui-Juan; Hou, Guo-Jiao; Zhu, Zhen-Gang; Yan, Qing-Bo; Su, Gang

    2016-01-01

    Organic-inorganic halide perovskite solar cells have enormous potential to impact the existing photovoltaic industry. As realizing a higher conversion efficiency of the solar cell is still the most crucial task, a great number of schemes were proposed to minimize the carrier loss by optimizing the electrical properties of the perovskite solar cells. Here, we focus on another significant aspect that is to minimize the light loss by optimizing the light management to gain a high efficiency for perovskite solar cells. In our scheme, the slotted and inverted prism structured SiO2 layers are adopted to trap more light into the solar cells, and a better transparent conducting oxide layer is employed to reduce the parasitic absorption. For such an implementation, the efficiency and the serviceable angle of the perovskite solar cell can be promoted impressively. This proposal would shed new light on developing the high-performance perovskite solar cells. PMID:26733112

  7. Improvements leading to high efficiency alkaline electrolysis cells

    NASA Astrophysics Data System (ADS)

    Murray, J. N.; Yaffe, M. R.

    Recent progress in the improvement of the operating efficiency of alkaline water electrolysis cells for hydrogen production is discussed. Development efforts have been concentrated on the replacement of the conventional asbestos electrode separator by a porous electrode separator fabricated from polybenzimidazole, advanced electrode screening modules, extended duration tests of the improved electrocatalyst/electrode systems, and an engineering design and economics evaluation computer program designed to calculate the cost of hydrogen and the design of the cost-optimal electrolysis plant. The improvements undertaken thus far have resulted in an increase in cell voltage efficiency from less than 71% to 86% by the use of a new high-efficiency cathode structure and operation at higher temperatures and reasonably high current densities.

  8. Potential high efficiency solar cells: Applications from space photovoltaic research

    NASA Technical Reports Server (NTRS)

    Flood, D. J.

    1986-01-01

    NASA involvement in photovoltaic energy conversion research development and applications spans over two decades of continuous progress. Solar cell research and development programs conducted by the Lewis Research Center's Photovoltaic Branch have produced a sound technology base not only for the space program, but for terrestrial applications as well. The fundamental goals which have guided the NASA photovoltaic program are to improve the efficiency and lifetime, and to reduce the mass and cost of photovoltaic energy conversion devices and arrays for use in space. The major efforts in the current Lewis program are on high efficiency, single crystal GaAs planar and concentrator cells, radiation hard InP cells, and superlattice solar cells. A brief historical perspective of accomplishments in high efficiency space solar cells will be given, and current work in all of the above categories will be described. The applicability of space cell research and technology to terrestrial photovoltaics will be discussed.

  9. Overview of SERI's high efficiency solar cell research

    NASA Technical Reports Server (NTRS)

    Benner, J. P.; Cole, L. A.; Leboeuf, C. M.

    1985-01-01

    The bulk of the research efforts supported by the Solar Energy Research Institute (SERI) High Efficiency Concepts area has been directed towards establishing the feasibility of achieving very high efficiencies, 30% for concentrator and more than 20% for thin film flat plate, in solar cell designs which could possibly be produced competitively. The research has accomplished a great deal during the past two years. Even though the desired performance levels have not yet been demonstrated, based on the recent progress, a greater portion of the terrestrial photovoltaics community believes that these efficiencies are attainable. The program will now allocate a larger portion of resources to low cost, large area deposition technology. The program is currently shifting greater emphasis on to the study of crystal growth in order to provide the understanding and tools needed to design a large area process.

  10. High-efficiency coaxial relativistic backward wave oscillator.

    PubMed

    Teng, Yan; Xiao, Renzhen; Song, Zhimin; Jun, Sun; Chen, Changhua; Shao, Hao; Liu, Guozhi; Tang, Chuanxiang

    2011-02-01

    This paper studies the coaxial relativistic backward wave oscillator (CRBWO) through analytical, numerical, and experimental methods. This new type of device is remarked by its high efficiency of more than 35%, which is predicted by the theoretical calculation and the numerical simulation and validated by experiment. The two primary hindrances preventing CRBWO from achieving the expected high efficiency, the poor coaxiality and the power capacity, are discussed in detail and some advanced methods are developed. The theoretical and numerical conclusions agree with the experiment results, which are obtained from the electric probe and the calorimeter simultaneously for each shot of CRBWO. Employing the electron beam pulse of the full width at half maximum 28 ns, a microwave pulse of the width about 20 ns is generated in the experiment; the power is 710 MW and the efficiency is higher than 33%.

  11. Lamellar multilayer gratings with very high diffraction efficiency

    SciTech Connect

    Martynov, V.V. |; Yakshin, A.; Agafonov, Yu.A.; Padmore, H.A.

    1997-07-01

    The authors report here the development of a hard x-ray multilayer grating that has achieved an absolute efficiency of 34% at a wavelength of 1.54{angstrom}. The W-C multilayer itself has a reflectivity of 57% and the grating has a 0th order absolute efficiency of 36%. The origin of this extraordinarily high efficiency is that the short period and highly asymmetric structure of the gratings combined with its deep grooves allows light to interact with a large number of layer pairs. This increases angular separation of the diffraction orders and reduces the multilayer bandwidth to the point where there is little or no order to order overlap in the grating structure, and hence maximum intensity can be diffracted into a selected order. This paper reports on the development of an optimized multilayer grating and some of its unique characteristics.

  12. Highly efficient light management for perovskite solar cells

    NASA Astrophysics Data System (ADS)

    Wang, Dong-Lin; Cui, Hui-Juan; Hou, Guo-Jiao; Zhu, Zhen-Gang; Yan, Qing-Bo; Su, Gang

    2016-01-01

    Organic-inorganic halide perovskite solar cells have enormous potential to impact the existing photovoltaic industry. As realizing a higher conversion efficiency of the solar cell is still the most crucial task, a great number of schemes were proposed to minimize the carrier loss by optimizing the electrical properties of the perovskite solar cells. Here, we focus on another significant aspect that is to minimize the light loss by optimizing the light management to gain a high efficiency for perovskite solar cells. In our scheme, the slotted and inverted prism structured SiO2 layers are adopted to trap more light into the solar cells, and a better transparent conducting oxide layer is employed to reduce the parasitic absorption. For such an implementation, the efficiency and the serviceable angle of the perovskite solar cell can be promoted impressively. This proposal would shed new light on developing the high-performance perovskite solar cells.

  13. Highly efficient solar cells based on poly(3-butylthiophene) nanowires.

    PubMed

    Xin, Hao; Kim, Felix Sunjoo; Jenekhe, Samson A

    2008-04-23

    Poly(3-butylthiophene) (P3BT) nanowires, prepared by solution-phase self-assembly, have been used to construct highly efficient P3BT/fullerene nanocomposite solar cells. The fullerene/P3BT nanocomposite films showed an electrically bicontinuous nanoscale morphology with average field-effect hole mobilities as high as 8.0 x 10(-3) cm2/Vs due to the interconnected P3BT nanowire network revealed by TEM and AFM imaging. The power conversion efficiency of fullerene/P3BT nanowire devices was 3.0% (at 100 mW/cm2, AM1.5) in air and found to be identical with our similarly tested fullerene/poly(3-hexylthiophene) photovoltaic cells. This discovery expands the scope of promising materials and architectures for efficient bulk heterojunction solar cells.

  14. Progress in efficiency-optimized high-power diode lasers

    NASA Astrophysics Data System (ADS)

    Pietrzak, A.; Hülsewede, R.; Zorn, M.; Hirsekorn, O.; Sebastian, J.; Meusel, J.; Hennig, P.; Crump, P.; Wenzel, H.; Knigge, S.; Maaßdorf, A.; Bugge, F.; Erbert, G.

    2013-10-01

    High-power diode lasers are highly efficient sources of optical energy for industrial and defense applications, either directly or as pump sources for solid state or fiber lasers. We review here how advances in diode laser design and device technology have enabled the performance to be continuously improved. An overview is presented of recent progress at JENOPTIK in the development of commercial diode lasers optimized for peak performance, robust high-yield manufacture and long lifetimes. These diode lasers are tailored to simultaneously operate with reduced vertical carrier leakage, low thermal and electrical resistance and low optical losses. In this way, the highest electro-optical efficiencies are sustained to high currents. For example, 940-nm bars with high fill factor are shown to deliver continuous wave (CW) output powers of 280 W with conversion efficiency of < 60%. These bars have a vertical far field angle with 95% power content of just 40°. In addition, 955-nm single emitters with 90μm stripe width deliver 12 W CW output with power conversion efficiency at the operating point of 69%. In parallel, the Ferdinand-Braun-Institut (FBH) is working to enable the next generation of high power diode lasers, by determining the key limitations to performance and by pioneering new technologies to address these limits. An overview of recent studies at the FBH will therefore also be presented. Examples will include structures with further reduced far field angles, higher lateral beam quality and increased peak power and efficiency. Prospects for further performance improvement will be discussed.

  15. Inhibition of intracellular antiviral defense mechanisms augments lentiviral transduction of human natural killer cells: implications for gene therapy.

    PubMed

    Sutlu, Tolga; Nyström, Sanna; Gilljam, Mari; Stellan, Birgitta; Applequist, Steven E; Alici, Evren

    2012-10-01

    Adoptive immunotherapy with genetically modified natural killer (NK) cells is a promising approach for cancer treatment. Yet, optimization of highly efficient and clinically applicable gene transfer protocols for NK cells still presents a challenge. In this study, we aimed at identifying conditions under which optimum lentiviral gene transfer to NK cells can be achieved. Our results demonstrate that stimulation of NK cells with interleukin (IL)-2 and IL-21 supports efficient transduction using a VSV-G pseudotyped lentiviral vector. Moreover, we have identified that inhibition of innate immune receptor signaling greatly enhances transduction efficiency. We were able to boost the efficiency of lentiviral genetic modification on average 3.8-fold using BX795, an inhibitor of the TBK1/IKKɛ complex acting downstream of RIG-I, MDA-5, and TLR3. We have also observed that the use of BX795 enhances lentiviral transduction efficiency in a number of human and mouse cell lines, indicating a broadly applicable, practical, and safe approach that has the potential of being applicable to various gene therapy protocols.

  16. 40 CFR 761.71 - High efficiency boilers.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... PROHIBITIONS Storage and Disposal § 761.71 High efficiency boilers. (a) To burn mineral oil dielectric fluid... percent (on a volume basis) of the total fuel feed rate. (v) The mineral oil dielectric fluid is not fed... feeding these fluids during either start up or shut down operations). (vi) The owner or operator of...

  17. Basic studies of 3-5 high efficiency cell components

    SciTech Connect

    Lundstrom, M.S.; Melloch, M.R.; Pierret, R.F.; Carpenter, M.S.; Chuang, H.L.; Dodd, P.E.; Keshavarzi, A.; Klausmeier-Brown, M.E.; Lush, G.B.; Stellwag, T.B. )

    1993-01-01

    This project's objective is to improve our understanding of the generation, recombination, and transport of carriers within III-V homo- and heterostructures. The research itself consists of fabricating and characterizing solar cell building blocks'' such as junctions and heterojunctions as well as basic measurements of material parameters. A significant effort is also being directed at characterizing loss mechanisms in high-quality, III-V solar cells fabricated in industrial research laboratories throughout the United States. The project's goal is to use our understanding of the device physics of high-efficiency cell components to maximize cell efficiency. A related goal is the demonstration of new cell structures fabricated by molecular beam epitaxy (MBE). The development of measurement techniques and characterization methodologies is also a project objective. This report describes our progress during the fifth and final year of the project. During the past five years, we've teamed a great deal about heavy doping effects in p[sup +] and n[sup +] GaAs and have explored their implications for solar cells. We have developed an understanding of the dominant recombination losses in present-day, high-efficiency cells. We've learned to appreciated the importance of recombination at the perimeter of the cell and have developed techniques for chemically passivating such edges. Finally, we've demonstrated that films grown by molecular beam epitaxy are suitable for high-efficiency cell research.

  18. Novel Semiconducting Polymers for Highly Efficient Solar Energy Harvesting

    DTIC Science & Technology

    2014-03-11

    energy PCE to polymers disclosed in literature in BHJ polymer solar cells in combination with fullerene derivatives as acceptors. Solar power... fullerene derivatives are the best candidates so far due to their high electron affinity, superior electron mobility and their three dimensional...structure, providing unique packing ability in blend to efficiently form electron transport channels. 13 The original fullerenes do not have enough

  19. Development of High Efficiency (14%) Solar Cell Array Module

    NASA Technical Reports Server (NTRS)

    Iles, P. A.; Khemthong, S.; Olah, S.; Sampson, W. J.; Ling, K. S.

    1979-01-01

    High efficiency solar cells required for the low cost modules was developed. The production tooling for the manufacture of the cells and modules was designed. The tooling consisted of: (1) back contact soldering machine; (2) vacuum pickup; (3) antireflective coating tooling; and (4) test fixture.

  20. Applications of ion implantation for high efficiency silicon solar cells

    NASA Technical Reports Server (NTRS)

    Minnucci, J. A.; Kirkpatrick, A. R.

    1977-01-01

    Ion implantation is utilized for the dopant introduction processes necessary to fabricate a silicon solar cell. Implantation provides a versatile powerful tool for development of high efficiency cells. Advantages and problems of implantation and the present status of developmental use of the technique for solar cells are discussed.

  1. High-quantum efficiency, long-lived luminescing refractory oxides

    DOEpatents

    Chen, Y.; Gonzalez, R.; Summers, G.P.

    A crystal having a high-quantum efficiency and a long period of luminescence is formed of MgO or CaO and possessing a concentration ratio of H/sup -/ ions to F centers in the range of about 0.05 to about 10.

  2. High-quantum efficiency, long-lived luminescing refractory oxides

    DOEpatents

    Chen, Yok; Gonzalez, Roberto; Summers, Geoffrey P.

    1984-01-01

    A crystal having a high-quantum efficiency and a long period of luminescence is formed of an oxide selected from the group consisting of magnesium oxide and calcium oxide and possessing a concentration ratio of H.sup.- ions to F centers in the range of about 0.05 to about 10.

  3. Super Boiler: First Generation, Ultra-High Efficiency Firetube Boiler

    SciTech Connect

    2006-06-01

    This factsheet describes a research project whose goal is to develop and demonstrate a first-generation ultra-high-efficiency, ultra-low emissions, compact gas-fired package boiler (Super Boiler), and formulate a long-range RD&D plan for advanced boiler technology out to the year 2020.

  4. High efficiency solar cells for laser power beaming applications

    NASA Technical Reports Server (NTRS)

    Jain, Raj K.; Landis, G. A.

    1995-01-01

    Understanding solar cell response to pulsed laser outputs is important for the evaluation of power beaming applications. The time response of high efficiency GaAs and silicon solar cells to a 25 nS monochromatic pulse input is described. The PC-1D computer code is used to analyze the cell current during and after the pulse for various conditions.

  5. Development of a high efficiency thin silicon solar cell

    NASA Technical Reports Server (NTRS)

    Lindmayer, J.

    1975-01-01

    Specific power output and radiation resistance characteristics developed for thin film silicon solar cells are reported. The technological base for fabricating these high efficiency cells and limitations of cell photovoltage are included. In addition, optical and electronic measurement instrumentation and mathematical analyses aids are included. Antireflection coatings for these cells are discussed.

  6. LTC1877 High Efficiency Regulator Total Ionizing Dose Test Report

    NASA Technical Reports Server (NTRS)

    Oldham, Timothy; Pellish, Jonathan; Boutte, Alvin

    2012-01-01

    This report presents total ionizing dose evaluation data for the Linear Technology Corporation LTC1877 high efficiency monolithic synchronous step-down regulator. Data sheet parameters were tracked as a function of ionizing dose up to a total of 20 krad(SiO2). Control devices were also used.

  7. Field testing of high-efficiency supermarket refrigeration

    SciTech Connect

    Walker, D. )

    1992-12-01

    The Electric Power Research Institute (EPRI) has undertaken a field test to quantify the performance of high-efficiency supermarket refrigeration. The initial work on this project was presented in EPRI report CU-6268 Supermarket Refrigeration Modeling and Field Demonstration.'' The information given here was generated through continued testing at the field test site. The field test was conducted at a supermarket owned by Safeway Stores, Inc., that was located in Menlo Park, CA. Testing was performed with the existing conventional refrigeration system and a high-efficiency multiplex refrigeration system that was installed for these tests. The results of the testing showed that the high-efficiency multiplex system reduced refrigeration energy consumption by 23.9% and peak electric demand for refrigeration by 30.0%. Analyses of these savings showed that the largest portion was due to the use of high-efficiency compressors (29.5% of total saving). Floating head pressure control, ambient and mechanical subcooling, compressor multiplexing and hot gas defrost accounted for 50% of total savings. The remainder of the savings (20.5%) were attributed to the use of an evaporative condenser. Tests were also conducted with several retrofit technologies. The most promising results were obtained with external liquid-suction heat exchangers installed at the outlets of the display cases. Favorable paybacks were calculated for these exchangers when they were used with very low and low temperature refrigeration.

  8. Field testing of high-efficiency supermarket refrigeration. Final report

    SciTech Connect

    Walker, D.

    1992-12-01

    The Electric Power Research Institute (EPRI) has undertaken a field test to quantify the performance of high-efficiency supermarket refrigeration. The initial work on this project was presented in EPRI report CU-6268 ``Supermarket Refrigeration Modeling and Field Demonstration.`` The information given here was generated through continued testing at the field test site. The field test was conducted at a supermarket owned by Safeway Stores, Inc., that was located in Menlo Park, CA. Testing was performed with the existing conventional refrigeration system and a high-efficiency multiplex refrigeration system that was installed for these tests. The results of the testing showed that the high-efficiency multiplex system reduced refrigeration energy consumption by 23.9% and peak electric demand for refrigeration by 30.0%. Analyses of these savings showed that the largest portion was due to the use of high-efficiency compressors (29.5% of total saving). Floating head pressure control, ambient and mechanical subcooling, compressor multiplexing and hot gas defrost accounted for 50% of total savings. The remainder of the savings (20.5%) were attributed to the use of an evaporative condenser. Tests were also conducted with several retrofit technologies. The most promising results were obtained with external liquid-suction heat exchangers installed at the outlets of the display cases. Favorable paybacks were calculated for these exchangers when they were used with very low and low temperature refrigeration.

  9. High-efficiency heteroepitaxial solar cells for space power applications

    NASA Technical Reports Server (NTRS)

    Vernon, S. M.; Tobin, S. P.; Keavney, C. J.; Wojtczuk, S. J.

    1989-01-01

    The experimental results for several technical approaches aimed at achieving highly efficient solar cells for space-power applications are reported. Efficiencies of up to 24.5 percent (170X, AM0) and 21.7 percent (1X, AM0) have been achieved with homoepitaxial GaAs p/n cells. This one-sun AM0 efficiency value is believed to be the highest reported to date. Tandem solar cells utilizing GaAs-on-Ge structures have been fabricated and shown to have efficiencies up to 21.3 percent (1X, AM0), and a GaAs-on-Si cell at 15.2 percent (1X, AM0) is reported. Homoepitaxial n/p InP cells with an efficiency of 18.8 percent (1X, AM0) are also reported. The fabrication of heteroepitaxial InP solar cells with one-sun AM0 efficiency values of 9.4 percent (on GaAs) and 7.2 percent (on Si) is described.

  10. Research on stable, high-efficiency amorphous silicon multijunction modules

    SciTech Connect

    Guha, S. )

    1991-12-01

    This report describes research to improve the understanding of amorphous silicon alloys and other relevant non-semiconductor materials for use in high-efficiency, large-area multijunction modules. The research produced an average subcell initial efficiency of 8.8% over a 1-ft{sup 2} area using same-band-gap, dual-junction cells deposited over a ZnO/AlSi back reflector. An initial efficiency of 9.6% was achieved using a ZnO/Ag back reflector over smaller substrates. A sputtering machine will be built to deposit a ZnO/Ag back reflector over a 1-ft{sup 2} area so that a higher efficiency can also be obtained on larger substrates. Calculations have been performed to optimize the grid pattern, bus bars, and cell interconnects on modules. With our present state of technology, we expect a difference of about 6% between the aperture-area and active-area efficiencies of modules. Preliminary experiments show a difference of about 8%. We can now predict the performance of single-junction cells after long-term light exposure at 50{degree}C by exposing cells to short-term intense light at different temperatures. We find that single-junction cells deposited on a ZnO/Ag back reflector show the highest stabilized efficiency when the thickness of the intrinsic layers is about 2000 {angstrom}. 8 refs.

  11. In vivo protein transduction to the CNS.

    PubMed

    Loftus, L T; Li, H-F; Gray, A J; Hirata-Fukae, C; Stoica, B A; Futami, J; Yamada, H; Aisen, P S; Matsuoka, Y

    2006-01-01

    Proteins and peptides are useful research and therapeutic tools, however applications are limited because delivery to the desired location is not easily achievable. There are two hurdles in protein/peptide delivery to the brain: the blood-brain barrier and intracellular penetration. Penetration to both brain and the intracellular space can be achieved by adjusting hydrophilicity, and small molecule pharmacological agents have been successfully developed using this approach. But with proteins and peptides, it is difficult to modify the hydrophilicity without influencing biological functions. Trans-acting factor protein from the human immunodeficiency virus contains a highly conserved cationic peptide sequence necessary for transduction across the cell membrane. While trans-acting factor peptide has been used for in vitro protein transduction, its in vivo application is very limited because it is rapidly degraded by proteolysis. Polyethylenimine is a chemically synthesized small molecule cationization agent; the charge density is greater than a peptide-based cationic cluster such as trans-acting factor, and it is resistant to proteolysis in vivo. We first tested intracellular protein transduction following direct brain injection in mice using polyethylenimine-conjugated green fluorescence protein and beta-galactosidase (molecular weights 29 and 540 kDa, respectively). Polyethylenimine-conjugates penetrated to the intracellular space immediately surrounding the injection site within one hour. We further tested polyethylenimine-mediated protein transduction following intranasal administration, which bypasses the blood-brain barrier. Polyethylenimine-conjugates in pH 7.5 solution did not reach the brain, probably because the polyethylenimine-conjugates penetrated into the intracellular space where first exposed to the tissue, i.e. at the nasal mucosae. We temporarily reduced the electrostatic interaction between cationized polyethylenimine-conjugates and cellular

  12. Novel HDAd/EBV Reprogramming Vector and Highly Efficient Ad/CRISPR-Cas Sickle Cell Disease Gene Correction

    PubMed Central

    Li, Chao; Ding, Lei; Sun, Chiao-Wang; Wu, Li-Chen; Zhou, Dewang; Pawlik, Kevin M.; Khodadadi-Jamayran, Alireza; Westin, Erik; Goldman, Frederick D.; Townes, Tim M.

    2016-01-01

    CRISPR/Cas enhanced correction of the sickle cell disease (SCD) genetic defect in patient-specific induced Pluripotent Stem Cells (iPSCs) provides a potential gene therapy for this debilitating disease. An advantage of this approach is that corrected iPSCs that are free of off-target modifications can be identified before differentiating the cells into hematopoietic progenitors for transplantation. In order for this approach to be practical, iPSC generation must be rapid and efficient. Therefore, we developed a novel helper-dependent adenovirus/Epstein-Barr virus (HDAd/EBV) hybrid reprogramming vector, rCLAE-R6, that delivers six reprogramming factors episomally. HDAd/EBV transduction of keratinocytes from SCD patients resulted in footprint-free iPSCs with high efficiency. Subsequently, the sickle mutation was corrected by delivering CRISPR/Cas9 with adenovirus followed by nucleoporation with a 70 nt single-stranded oligodeoxynucleotide (ssODN) correction template. Correction efficiencies of up to 67.9% (βA/[βS+βA]) were obtained. Whole-genome sequencing (WGS) of corrected iPSC lines demonstrated no CRISPR/Cas modifications in 1467 potential off-target sites and no modifications in tumor suppressor genes or other genes associated with pathologies. These results demonstrate that adenoviral delivery of reprogramming factors and CRISPR/Cas provides a rapid and efficient method of deriving gene-corrected, patient-specific iPSCs for therapeutic applications. PMID:27460639

  13. Required material properties for high-efficiency CIGS modules

    NASA Astrophysics Data System (ADS)

    Repins, Ingrid; Glynn, Stephen; Duenow, Joel; Coutts, Timothy J.; Metzger, Wyatt K.; Contreras, Miguel A.

    2009-08-01

    Relatively high proven efficiencies of CIGS devices are often cited regarding its choice as a semiconductor for photovoltaic manufacturing. Module efficiency is an important parameter, as a number of factors in the cost per watt are driven downward by increasing efficiency. Some of these factors include materials costs, throughput for a given capital investment, and installation costs. Thus, realizing high-efficiency (e.g. 15%) large-area CIGS modules is key in both reducing cost per watt and differentiating the technology from other thin films. This paper discusses the material properties required of each layer of the CIGS device such that large-area CIGS modules can achieve efficiencies 15%, which is substantially higher than the current industrial state-of-the-art. The sensitivity of module performance to the important material parameters is quantified based on both experimental data and modeling. Necessary performance differences between small-area devices and large-area modules imposed by geometry are also quantified. Potential technical breakthroughs that may relax the requirements for each layer are discussed.

  14. Ultra-Compact High-Efficiency Luminaire for General Illumination

    SciTech Connect

    Lowes, Ted

    2012-04-08

    Cree has developed a new ultra-compact light emitting diode (LED) luminaire capable of providing high efficacy with excellent color quality that can lead to significant energy savings in today's commercial and retail applications. This success was based on an integrated development effort tailoring the LED component characteristics, optics, thermal management and driver design for the small footprint to achieve an overall system efficiency of 70%. A new LED component was designed to provide high brightness and efficacy in a form factor that allowed for a small directional beam with a luminaire housing design able to dissipate the heat effectively using a small physical envelope. A very compact, 90% efficient driver was developed to meet the system performance while not taking away any thermal mass from the heat sink. A 91% efficient secondary optics was designed to maximize efficiency while providing a smooth beam. The reliability of the new LED component was robust under accelerated testing conditions. Luminaires were assembled integrating the novel LED component, secondary optics, heat sink and driver technology to demonstrate the system improvement. Cree has successfully completed this project by developing an ultra-compact LED luminaire that provided 380 lumens at a correlated color temperature (CCT) of 2822 K and color rendering index (CRI) of 94 with an efficacy of 94 lumens per watt (LPW) when operating at 4 W input power (steady state) with an overall system efficiency of 81%. At a higher input power of 9 Watts, the lamp provided 658 lumens at 71 LPW.

  15. Encapsulation, with high efficiency, of radioactive metal ions in liposomes.

    PubMed

    Hwang, K J; Merriam, J E; Beaumier, P L; Luk, K F

    1982-05-05

    The encapsulation of radioactive metalic cations, such as 111In3+ or 67Ga3+, in the internal aqueous compartment of liposomes can be achieved with an efficiency of about 90%. The efficient loading of a high specific activity of cations into liposomes involves the transport of 111In3+ or 67Ga3+ through the lipid bilayer to an encapsulated strong chelate, such as nitrilotriacetic acid, by 8-hydroxyquinoline, in conjunction with an efficient anion-exchange resin technique for the removal of the external cations. The efficiency of loading cations to liposomes is affected markedly by the concentration of 8-hydroxyquinoline-metal, and the presence of the chelating agents in the loading incubation mixture. However, the loading efficiency is not affected by the pH of the internal aqueous compartment of liposomes over a range of pH 5-9, the concentration of the liposomes, the method of liposomal preparation, the lamellar structure of the liposomes, and the composition of liposomes. Furthermore, the loading procedures do not appear to affect the size and the permeability of liposomes. There is a good agreement in the tissue distributions of the liposomes prepared by the present loading methods and those by the conventional method of encapsulation by sonication. Liposomes entrapping high specific activity of 67Ga3+ or 111In3+ will be useful for future studies of the in vivo kinetics of liposomes by the combined techniques of scintigraphic imaging and the gamma-ray perturbed angular correlation.

  16. Multi-wavelength high efficiency laser system for lidar applications

    NASA Astrophysics Data System (ADS)

    Willis, Christina C. C.; Culpepper, Charles; Burnham, Ralph

    2015-09-01

    Motivated by the growing need for more efficient, high output power laser transmitters, we demonstrate a multi-wavelength laser system for lidar-based applications. The demonstration is performed in two stages, proving energy scaling and nonlinear conversion independently for later combination. Energy scaling is demonstrated using a 1064 nm MOPA system which employs two novel ceramic Nd:YAG slab amplifiers, the structure of which is designed to improve the amplifier's thermal performance and energy extraction via three progressive doping stages. This structure improved the extraction efficiency by 19% over previous single-stage dopant designs. A maximum energy of 34 mJ was produced at 500 Hz with a 10.8 ns pulse duration. High efficiency non-linear conversion from 1064 nm to 452 nm is demonstrated using a KTP ring OPO with a BBO intra-cavity doubler pumped with 50 Hz, 16 ns 1064 nm pulses. The OPO generates 1571 nm signal which is frequency doubled to 756 nm by the BBO. Output 786 nm pulses are mixed with the 1064 nm pump pulses to generate 452 nm. A conversion efficiency of 17.1% was achieved, generating 3 mJ of 452 nm pulses of 7.8 ns duration. Pump power was limited by intra-cavity damage thresholds, and in future experiments we anticipate >20% conversion efficiency.

  17. Development of a high efficiency thin silicon solar cell

    NASA Technical Reports Server (NTRS)

    Storti, G.; Culik, J.; Wrigley, C.

    1980-01-01

    Significant improvements in open-circuit voltage and conversion efficiency, even on relatively high bulk resistivity silicon, were achieved by using a screen-printed aluminum paste back surface field. A 4 sq cm 50 micron m thick cell was fabricated from textured 10 omega-cm silicon which had an open-circuit voltage of 595 mV and AMO conversion efficiency at 25 C of 14.3%. The best 4 sq cm 50 micron thick cell (2 omega-cm silicon) produced had an open-circuit voltage of 607 mV and an AMO conversion efficiency of 15%. Processing modifications are described which resulted in better front contact integrity and reduced breakage. These modifications were utilized in the thin cell pilot line to fabricate 4 sq cm cells with an average AMO conversion efficiency at 25 C of better than 12.5% and with lot yields as great as 51% of starts; a production rate of 10,000 cells per month was demonstrated. A pilot line was operated which produced large area (25 cm) ultra-thin cells with an average AMO conversion efficiency at 25 deg of better than 11.5% and a lot yield as high as 17%.

  18. High Efficient OLEDs and Their Application to Lighting

    NASA Astrophysics Data System (ADS)

    Komoda, Takuya; Ide, Nobuhiro; Kido, Junji

    Organic Light Emitting Diode (OLED) is one of the strongest candidates for the next generation solid state lighting alternative to conventional incandescent bulbs and fluorescent lamps. There are still a lot of issues to overcome in order to commercialize OLED lighting, but a number of elemental technologies indispensable for OLED lighting such as high efficiency, long lifetime at high luminance and large area uniform emission have been developed.

  19. Efficient ARQ scheme for high error rate channels

    NASA Astrophysics Data System (ADS)

    Moeneclaey, M.; Bruneel, H.

    1984-11-01

    A continuous error detection and retransmission (ARQ) protocol which preserves the order of the data block is presented. Data blocks are retransmitted continuously until a positive acknowledgement is received. The next block is then transmitted. It is shown that in high error rate conditions, i.e., the probability of message error is greater than 0.5, the scheme is more efficient than go-back-N and selective repeat schemes. The high error rates increase with the length of propagation delays.

  20. A high efficiency 3 kW switchmode battery charger

    NASA Technical Reports Server (NTRS)

    Latos, T. S.; Bosack, D. J.

    1982-01-01

    This paper discusses the design approach and status of a high-efficiency switchmode battery charger designed to charge a 108 V battery from the 115 Vac line. The charger contains a transformer isolated boost chopper operating at 20 kHz. The boost inductor current is programmed to follow the ac line voltage such that high power factor operation and low line distortion are obtained.

  1. A Cooperative Interface for Highly Efficient Lithium-Sulfur Batteries.

    PubMed

    Peng, Hong-Jie; Zhang, Ze-Wen; Huang, Jia-Qi; Zhang, Ge; Xie, Jin; Xu, Wen-Tao; Shi, Jia-Le; Chen, Xiang; Cheng, Xin-Bing; Zhang, Qiang

    2016-11-01

    A cooperative interface constructed by "lithiophilic" nitrogen-doped graphene frameworks and "sulfiphilic" nickel-iron layered double hydroxides (LDH@NG) is proposed to synergistically afford bifunctional Li and S binding to polysulfides, suppression of polysulfide shuttles, and electrocatalytic activity toward formation of lithium sulfides for high-performance lithium-sulfur batteries. LDH@NG enables high rate capability, long lifespan, and efficient stabilization of both sulfur and lithium electrodes.

  2. Evidence for the plasticity of arthropod signal transduction pathways.

    PubMed

    Pace, Ryan M; Eskridge, P Cole; Grbić, Miodrag; Nagy, Lisa M

    2014-12-01

    Metazoans are known to contain a limited, yet highly conserved, set of signal transduction pathways that instruct early developmental patterning mechanisms. Genomic surveys that have compared gene conservation in signal transduction pathways between various insects and Drosophila support the conclusion that these pathways are conserved in evolution. However, the degree to which individual components of signal transduction pathways vary among more divergent arthropods is not known. Here, we report our results of a survey of the genome of the two-spotted spider mite Tetranychus urticae, using a set of 294 Drosophila orthologs of genes that function in signal transduction. We find a third of all genes surveyed absent from the spider mite genome. We also identify several novel duplications that have not been previously reported for a chelicerate. In comparison with previous insect surveys, Tetranychus contains a decrease in overall gene conservation, as well as an unusual ratio of ligands to receptors and other modifiers. These findings suggest that gene loss and duplication among components of signal transduction pathways are common among arthropods and suggest that signal transduction pathways in arthropods are more evolutionarily labile than previously hypothesized.

  3. Efficiency and Loading Evaluation of High Efficiency Mist Eliminators (HEME) - 12003

    SciTech Connect

    Giffin, Paxton K.; Parsons, Michael S.; Waggoner, Charles A.

    2012-07-01

    High efficiency mist eliminators (HEME) are filters primarily used to remove moisture and/or liquid aerosols from an air stream. HEME elements are designed to reduce aerosol and particulate load on primary High Efficiency Particulate Air (HEPA) filters and to have a liquid particle removal efficiency of approximately 99.5% for aerosols down to sub-micron size particulates. The investigation presented here evaluates the loading capacity of the element in the absence of a water spray cleaning system. The theory is that without the cleaning system, the HEME element will suffer rapid buildup of solid aerosols, greatly reducing the particle loading capacity. Evaluation consists of challenging the element with a waste surrogate dry aerosol and di-octyl phthalate (DOP) at varying intervals of differential pressure to examine the filtering efficiency of three different element designs at three different media velocities. Also, the elements are challenged with a liquid waste surrogate using Laskin nozzles and large dispersion nozzles. These tests allow the loading capacity of the unit to be determined and the effectiveness of washing down the interior of the elements to be evaluated. (authors)

  4. Transduction of Gal+ by Coliphage T1 III. Requirement for Transcription and Translation in Recipient Cells

    PubMed Central

    Drexler, Henry

    1973-01-01

    A 10- to 15-min derepression of a λ prophage in a Gal− recipient during early infection with a transducing lysate of coliphage T1am will cause an increase in the efficiency of transduction of Gal+. An increase in the efficiency of transduction occurs when the donor is either nonlysogenic or lysogenic for λ; the increase is blocked by rifampin or chloramphenicol. With strain R901 it has been shown that efficient transduction can be blocked by treatment with rifampin after all chloramphenicol-sensitive steps have occurred. PMID:4587757

  5. Development of high efficiency (14 percent) solar cell array module

    NASA Technical Reports Server (NTRS)

    Iles, P. A.; Khemthong, S.; Olah, S.; Sampson, W. J.; Ling, K. S.

    1980-01-01

    Most effort was concentrated on development of procedures to provide large area (3 in. diameter) high efficiency (16.5 percent AM1, 28 C) P+NN+ solar cells. Intensive tests with 3 in. slices gave consistently lower efficiency (13.5 percent). The problems were identified as incomplete formation of and optimum back surface field (BSF), and interaction of the BSF process and the shallow P+ junction. The problem was shown not to be caused by reduced quality of silicon near the edges of the larger slices.

  6. High efficiency inorganic/organic hybrid tandem solar cells.

    PubMed

    Seo, Ji Hoon; Kim, Dong-Ho; Kwon, Se-Hun; Song, Myungkwan; Choi, Min-Seung; Ryu, Seung Yoon; Lee, Hyung Woo; Park, Yun Chang; Kwon, Jung-Dae; Nam, Kee-Seok; Jeong, Yongsoo; Kang, Jae-Wook; Kim, Chang Su

    2012-08-28

    Hybrid tandem solar cells comprising an inorganic bottom cell and an organic top cell have been designed and fabricated. The interlayer combination and thickness matching were optimized in order to increase the overall photovoltaic conversion efficiency. A maximum power conversion efficiency of 5.72% was achieved along with a V(oc) of 1.42 V, reaching as high as 92% of the sum of the subcell V(oc) values. Copyright © 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

  7. High-efficiency organic solar concentrators for photovoltaics.

    PubMed

    Currie, Michael J; Mapel, Jonathan K; Heidel, Timothy D; Goffri, Shalom; Baldo, Marc A

    2008-07-11

    The cost of photovoltaic power can be reduced with organic solar concentrators. These are planar waveguides with a thin-film organic coating on the face and inorganic solar cells attached to the edges. Light is absorbed by the coating and reemitted into waveguide modes for collection by the solar cells. We report single- and tandem-waveguide organic solar concentrators with quantum efficiencies exceeding 50% and projected power conversion efficiencies as high as 6.8%. The exploitation of near-field energy transfer, solid-state solvation, and phosphorescence enables 10-fold increases in the power obtained from photovoltaic cells, without the need for solar tracking.

  8. Very high X-ray efficiency from a blazed grating

    NASA Technical Reports Server (NTRS)

    Cash, W.; Kohnert, R.

    1982-01-01

    The efficiency of X-ray diffraction from reflection gratings has been investigated experimentally using a gold-coated plane grating of 25 x 120 mm with a nominal blaze angle of 21 deg in a conical diffraction mount. Results show that conical diffraction is capable of producing very high dispersion efficiencies. The peak of first order at 13.3 A is 40%; at 44 A, the peak is 34%. The most immediate application of this result is to X-ray astronomy where sources are weak.

  9. High efficiency fuel cell/advanced turbine power cycles

    SciTech Connect

    Morehead, H.

    1995-10-19

    An outline of the Westinghouse high-efficiency fuel cell/advanced turbine power cycle is presented. The following topics are discussed: The Westinghouse SOFC pilot manufacturing facility, cell scale-up plan, pressure effects on SOFC power and efficiency, sureCell versus conventional gas turbine plants, sureCell product line for distributed power applications, 20 MW pressurized-SOFC/gas turbine power plant, 10 MW SOFC/CT power plant, sureCell plant concept design requirements, and Westinghouse SOFC market entry.

  10. High-efficiency, 200 watt, 12-gigahertz traveling wave tube

    NASA Technical Reports Server (NTRS)

    Kosmahl, H. G.; Mcnary, B. D.; Sauseng, O.

    1974-01-01

    Design and performance of a highly efficient experimental 200-watt traveling wave tube for space communications are described. The tube uses a coupled cavity slow wave structure with periodic permanent magnet focusing. A two-step velocity taper is incorporated in the slow wave structure for velocity resynchronization with the modulated beam. The spent beam is reconditioned in a refocusing section before it is collected in a novel multistage depressed collector. The collector is radiation cooled and heat insulated from the tube body. At saturation the tube provides peak output power of 240 watts with a 35-db gain and an overall maximum efficiency of 56 percent.

  11. High-efficiency heteroepitaxial InP solar cells

    NASA Technical Reports Server (NTRS)

    Wanlass, M. W.; Coutts, T. J.; Ward, J. S.; Emery, K. A.

    1991-01-01

    High-efficiency, thin-film InP solar cells grown heteroepitaxially on GaAs and Si single-crystal bulk substrates are being developed as a means of eliminating the problems associated with using single-crystal InP substrates. A novel device structure employing a compositionally graded Ga(x)In(1-x)As layer between the bulk substrate and the InP cell layers is used to reduce the dislocation density and improve the minority carrier properties in the InP. The structures are grown in a continuous sequence of steps using computer-controlled atmospheric pressure metalorganic vapor phase epitaxy (APMOVPE). Dislocation densities as low as 3 x 10(exp 7) sq cm and minority carrier lifetimes as high as 3.3 ns are achieved in the InP layers with this method using both GaAs or Si substrates. Structures prepared in this fashion are also completely free of microcracks. These results represent a substantial improvement in InP layer quality when compared to heteroepitaxial InP prepared using conventional techniques such as thermally cycled growth and post-growth annealing. The present work is is concerned with the fabrication and characterization of high-efficiency, thin-film InP solar cells. Both one-sun and concentrator cells were prepared for device structures grown on GaAs substrates. One-cell cells have efficiencies as high as 13.7 percent at 25 C. However, results for the concentrator cells are emphasized. The concentrator cell performance is characterized as a function of the air mass zero (AM0) solar concentration ratio and operating temperature. From these data, the temperature coefficients of the cell performance parameters are derived as a function of the concentration ratio. Under concentration, the cells exhibit a dramatic increase in efficiency and an improved temperature coefficient of efficiency. At 25 C, a peak conversion efficiency of 18.9 percent is reported. At 80 C, the peak AM0 efficiency is 15.7 percent at 75.6 suns. These are the highest efficiencies yet

  12. High efficiency low cost monolithic module for SARSAT distress beacons

    NASA Technical Reports Server (NTRS)

    Petersen, Wendell C.; Siu, Daniel P.

    1992-01-01

    The program objectives were to develop a highly efficient, low cost RF module for SARSAT beacons; achieve significantly lower battery current drain, amount of heat generated, and size of battery required; utilize MMIC technology to improve efficiency, reliability, packaging, and cost; and provide a technology database for GaAs based UHF RF circuit architectures. Presented in viewgraph form are functional block diagrams of the SARSAT distress beacon and beacon RF module as well as performance goals, schematic diagrams, predicted performances, and measured performances for the phase modulator and power amplifier.

  13. Highly Efficient Transition Metal Nanoparticle Catalysts in Aqueous Solutions.

    PubMed

    Wang, Changlong; Ciganda, Roberto; Salmon, Lionel; Gregurec, Danijela; Irigoyen, Joseba; Moya, Sergio; Ruiz, Jaime; Astruc, Didier

    2016-02-24

    A ligand design is proposed for transition metal nanoparticle (TMNP) catalysts in aqueous solution. Thus, a tris(triazolyl)-polyethylene glycol (tris-trz-PEG) amphiphilic ligand, 2, is used for the synthesis of very small TMNPs with Fe, Co, Ni, Cu, Ru, Pd, Ag, Pt, and Au. These TMNP-2 catalysts were evaluated and compared for the model 4-nitrophenol reduction, and proved to be extremely efficient. High catalytic efficiencies involving the use of only a few ppm metal of PdNPs, RuNPs, and CuNPs were also exemplified in Suzuki-Miyaura, transfer hydrogenation, and click reactions, respectively.

  14. Highly efficient beam steering with a transparent metasurface.

    PubMed

    Wei, Zeyong; Cao, Yang; Su, Xiaopeng; Gong, Zhijie; Long, Yang; Li, Hongqiang

    2013-05-06

    We propose an ultra-thin planar metasurface with phase discontinuities for highly efficient beam steering. The effect benefits from the broadband transparency and flexible phase modulation of stacked metal/dielectric multi-layers that is perforated with coaxial annular apertures. Proof-of-principle experiments verify that an efficiency of 65% and a deflection angle of 18° at 10 GHz are achieved for the transmitted beam, which are also in good agreement with the finite-difference-method-in-time-domain (FDTD) simulations. The scheme shall be general for the design of beam-steering transmitters in all frequencies.

  15. Investigation on high transmission efficiency 7 × 1 pump combiner

    NASA Astrophysics Data System (ADS)

    Cao, Yang; Shi, Wei; Sheng, Quan; Fu, Shijie; Zhang, Haiwei; Bai, Xiaolei; Qi, Liang; Yao, Jianquan

    2016-12-01

    The 7×1 end-pumped combiner employing 105/125 μm multimode fibers as pump fibers is investigated. The theoretical analysis reveals that sufficient taper length and low refractive index of the capillary should be adopted to fabricate high transmission efficiency combiners. Based on the simulation results, we fabricate a 7×1 end-pumped pump combiner with an average transmission efficiency of 98.9% and a total return loss of 1.1‰. The measured internal operating temperature of this combiner indicates it can endure pump power of the order of kilowatts.

  16. High Efficiency Microwave Power Amplifier: From the Lab to Industry

    NASA Technical Reports Server (NTRS)

    Sims, William Herbert, III; Bell, Joseph L. (Technical Monitor)

    2001-01-01

    Since the beginnings of space travel, various microwave power amplifier designs have been employed. These included Class-A, -B, and -C bias arrangements. However, shared limitation of these topologies is the inherent high total consumption of input power associated with the generation of radio frequency (RF)/microwave power. The power amplifier has always been the largest drain for the limited available power on the spacecraft. Typically, the conversion efficiency of a microwave power amplifier is 10 to 20%. For a typical microwave power amplifier of 20 watts, input DC power of at least 100 watts is required. Such a large demand for input power suggests that a better method of RF/microwave power generation is required. The price paid for using a linear amplifier where high linearity is unnecessary includes higher initial and operating costs, lower DC-to-RF conversion efficiency, high power consumption, higher power dissipation and the accompanying need for higher capacity heat removal means, and an amplifier that is more prone to parasitic oscillation. The first use of a higher efficiency mode of power generation was described by Baxandall in 1959. This higher efficiency mode, Class-D, is achieved through distinct switching techniques to reduce the power losses associated with switching, conduction, and gate drive losses of a given transistor.

  17. Highly Flexible and Efficient Solar Steam Generation Device.

    PubMed

    Chen, Chaoji; Li, Yiju; Song, Jianwei; Yang, Zhi; Kuang, Yudi; Hitz, Emily; Jia, Chao; Gong, Amy; Jiang, Feng; Zhu, J Y; Yang, Bao; Xie, Jia; Hu, Liangbing

    2017-08-01

    Solar steam generation with subsequent steam recondensation has been regarded as one of the most promising techniques to utilize the abundant solar energy and sea water or other unpurified water through water purification, desalination, and distillation. Although tremendous efforts have been dedicated to developing high-efficiency solar steam generation devices, challenges remain in terms of the relatively low efficiency, complicated fabrications, high cost, and inability to scale up. Here, inspired by the water transpiration behavior of trees, the use of carbon nanotube (CNT)-modified flexible wood membrane (F-Wood/CNTs) is demonstrated as a flexible, portable, recyclable, and efficient solar steam generation device for low-cost and scalable solar steam generation applications. Benefitting from the unique structural merits of the F-Wood/CNTs membrane-a black CNT-coated hair-like surface with excellent light absorbability, wood matrix with low thermal conductivity, hierarchical micro- and nanochannels for water pumping and escaping, solar steam generation device based on the F-Wood/CNTs membrane demonstrates a high efficiency of 81% at 10 kW cm(-2) , representing one of the highest values ever-reported. The nature-inspired design concept in this study is straightforward and easily scalable, representing one of the most promising solutions for renewable and portable solar energy generation and other related phase-change applications. © 2017 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

  18. High Efficiency LED Lamp for Solid-State Lighting

    SciTech Connect

    James Ibbetson

    2006-12-31

    This report contains a summary of technical achievements during a three-year project to demonstrate high efficiency, solid-state lamps based on gallium nitride/silicon carbide light-emitting diodes. Novel chip designs and fabrication processes are described for a new type of nitride light-emitting diode with the potential for very high efficiency. This work resulted in the demonstration of blue light-emitting diodes in the one watt class that achieved up to 495 mW of light output at 350 mA drive current, corresponding to quantum and wall plug efficiencies of 51% and 45%, respectively. When combined with a phosphor in Cree's 7090 XLamp package, these advanced blue-emitting devices resulted in white light-emitting diodes whose efficacy exceeded 85 lumens per watt. In addition, up to 1040 lumens at greater than 85 lumens per watt was achieved by combining multiple devices to make a compact white lamp module with high optical efficiency.

  19. Consequences of high-frequency operation on EUV source efficiency

    NASA Astrophysics Data System (ADS)

    Sizyuk, Tatyana

    2017-08-01

    A potential problem of future extreme ultraviolet (EUV) sources, required for high volume manufacture regimes, can be related to the contamination of the chamber environment by products of preceding laser pulse/droplet interactions. Implementation of high, 100 kHz and higher, repetition rate of EUV sources using Sn droplets ignited with laser pulses can cause high accumulation of tin in the chamber in the form of vapor, fine mist, or fragmented clusters. In this work, the effects of the residual tin accumulation in the EUV chamber in dependence on laser parameters and mitigation system efficiency were studied. The effect of various pressures of tin vapor on the CO2 and Nd:YAG laser beam propagation and on the size, the intensity, and the resulting efficiency of the EUV sources was analyzed. The HEIGHTS 3D package was used for this analysis to study the effect of residual background pressure and spatial distribution on EUV photon emission and collection. It was found that background pressure in the range of 1-5 Pa does not significantly influence the EUV source produced by CO2 lasers. A larger volume with this pressure condition, however, can reduce the efficiency of the source. However, an optimized volume of mix with proper density could increase the efficiency of the sources produced by CO2 lasers.

  20. Selective transduction of astrocytic and neuronal CNS subpopulations by lentiviral vectors pseudotyped with Chikungunya virus envelope.

    PubMed

    Eleftheriadou, Ioanna; Dieringer, Michael; Poh, Xuan Ying; Sanchez-Garrido, Julia; Gao, Yunan; Sgourou, Argyro; Simmons, Laura E; Mazarakis, Nicholas D

    2017-04-01

    Lentiviral vectors are gene delivery vehicles that integrate into the host genome of dividing and non-dividing mammalian cells facilitating long-term transgene expression. Lentiviral vector versatility is greatly increased by incorporating heterologous viral envelope proteins onto the vector particles instead of the native envelope, conferring on these pseudotyped vectors a modified tropism and host range specificity. We investigated the pseudotyping efficiency of HIV-1 based lentiviral vectors with alphaviral envelope proteins from the Chikungunya Virus (CHIKV-G) and Sindbis Virus (SINV-G). Following vector production optimisation, titres for the CHIKV-G pseudotype were comparable to the VSV-G pseudotype but those for the SINV-G pseudotype were significantly lower. High titre CHIKV-G pseudotyped vector efficiently transduced various human and mouse neural cell lines and normal human astrocytes (NHA) in vitro. Although transduction was broad, tropism for NHAs was observed. In vivo stereotaxic delivery in striatum, thalamus and hippocampus respectively in the adult rat brain revealed localised transduction restricted to striatal astrocytes and hippocampal dentate granule neurons. Transduction of different subtypes of granule neurons from precursor to post-mitotic stages of differentiation was evident in the sub-granular zone and dentate granule cell layer. No significant inflammatory response was observed, but comparable to that of VSV-G pseudotyped lentiviral vectors. Robust long-term expression followed for three months post-transduction along with absence of neuroinflammation, coupled to the selective and unique neuron/glial tropism indicates that these vectors could be useful for modelling and gene therapy studies in the CNS.