Synchronous oscillation prior to disruption caused by kink modes in HL-2A tokamak plasmas

NASA Astrophysics Data System (ADS)

Jiang, M.; Hu, D.; Wang, X. G.; Shi, Z. B.; Xu, Y.; Chen, W.; Ding, X. T.; Zhong, W. L.; Dong, Y. B.; Ji, X. Q.; Zhang, Y. P.; Gao, J. M.; Li, J. X.; Yang, Z. C.; Li, Y. G.; Liu, Y.

2015-08-01

A class of evident MHD activities prior to major disruption has been observed during recent radiation induced disruptions of the HL-2A tokamak discharges. It can be named SOD, synchronous oscillations prior to disruption, characterized by synchronous oscillation of electron cyclotron emission (ECE), core soft x-ray, Mirnov coil, and {{D}α} radiation signals at the divertor plate. The SOD activity is mostly observed in a parametric regime where the poloidal beta is low enough before disruption, typically corresponding to those radiation-induced disruptions. It has been found that the m/n = 2/1 mode is dominant during the SODs, and consequently it is the drop of the mode frequency and the final mode locking that lead to thermal quench. The mode frequency before the mode locking corresponds to the toroidal rotation frequency of the edge plasma. It is also found that during SODs, the location of the q = 2 surface is moving outward, and most of the plasma current is enclosed within the surface. This demonstrates that the current channel lies inside the rational surface during SOD, and thus the resistive kink mode is unstable. Further analysis of the electron temperature perturbation structure shows that the plasma is indeed dominated by the resistive kink mode, with kink-like perturbation in the core plasma region. It suggests that it is the nonlinear growth of the m/n = 2/1 resistive kink mode and its higher order harmonics, rather than the spontaneous overlapping of multiple neighboring islands, that ultimately triggered the disruption.

Evidence for Transfer of Membranes from Mesenchymal Stem Cells to HL-1 Cardiac Cells.

PubMed

Boomsma, Robert A; Geenen, David L

2014-01-01

This study examined the interaction of mouse bone marrow mesenchymal stem cells (MSC) with cardiac HL-1 cells during coculture by fluorescent dye labeling and then flow cytometry. MSC were layered onto confluent HL-1 cell cultures in a 1 : 4 ratio. MSC gained gap junction permeant calcein from HL-1 cells after 4 hours which was partially reduced by oleamide. After 20 hours, 99% MSC gained calcein, unaffected by oleamide. Double-labeling HL-1 cells with calcein and the membrane dye DiO resulted in transfer of both calcein and DiO to MSC. When HL-1 cells were labeled with calcein and MSC with DiO, MSC gained calcein while HL-1 cells gained DiO. Very little fusion was observed since more than 90% Sca-1 positive MSC gained DiO from HL-1 cells while less than 9% gained gap junction impermeant CMFDA after 20 hours with no Sca-1 transfer to HL-1 cells. Time dependent transfer of membrane DiD was observed from HL-1 cells to MSC (100%) and vice versa (50%) after 20 hours with more limited transfer of CMFDA. These results demonstrate that MSC and HL-1 cells exchange membrane components which may account for some of the beneficial effect of MSC in the heart after myocardial infarction.

Study of the Polarization Strategy for Electron Cyclotron Heating Systems on HL-2M

NASA Astrophysics Data System (ADS)

Zhang, F.; Huang, M.; Xia, D. H.; Song, S. D.; Wang, J. Q.; Huang, B.; Wang, H.

2016-06-01

As important components integrated in transmission lines of electron cyclotron heating systems, polarizers are mainly used to obtain the desired polarization for highly efficient coupling between electron cyclotron waves and plasma. The polarization strategy for 105-GHz electron cyclotron heating systems of HL-2M tokamak is studied in this paper. Considering the polarizers need high efficiency, stability, and low loss to realize any polarization states, two sinusoidal-grooved polarizers, which include a linear polarizer and an elliptical polarizer, are designed with the coordinate transformation method. The parameters, the period p and the depth d, of two sinusoidal-grooved polarizers are optimized by a phase difference analysis method to achieve an almost arbitrary polarization. Finally, the optimized polarizers are manufactured and their polarization characteristics are tested with a low-power test platform. The experimental results agree well with the numerical calculations, indicating that the designed polarizers can meet the polarization requirements of the electron cyclotron heating systems of HL-2M tokamak.

HL-10 cockpit

NASA Technical Reports Server (NTRS)

1967-01-01

Cockpit of the HL-10 lifting body. The HL-10 was one of five heavyweight lifting-body designs flown at NASA's Flight Research Center (FRC--later Dryden Flight Research Center), Edwards, California, from July 1966 to November 1975 to study and validate the concept of safely maneuvering and landing a low lift-over-drag vehicle designed for reentry from space. Northrop Corporation built the HL-10 and M2-F2, the first two of the fleet of 'heavy' lifting bodies flown by the NASA Flight Research Center. The contract for construction of the HL-10 and the M2-F2 was $1.8 million. 'HL' stands for horizontal landing, and '10' refers to the tenth design studied by engineers at NASA's Langley Research Center, Hampton, Va. After delivery to NASA in January 1966, the HL-10 made its first flight on Dec. 22, 1966, with research pilot Bruce Peterson in the cockpit. Although an XLR-11 rocket engine was installed in the vehicle, the first 11 drop flights from the B-52 launch aircraft were powerless glide flights to assess handling qualities, stability, and control. In the end, the HL-10 was judged to be the best handling of the three original heavy-weight lifting bodies (M2-F2/F3, HL-10, X-24A). The HL-10 was flown 37 times during the lifting body research program and logged the highest altitude and fastest speed in the Lifting Body program. On Feb. 18, 1970, Air Force test pilot Peter Hoag piloted the HL-10 to Mach 1.86 (1,228 mph). Nine days later, NASA pilot Bill Dana flew the vehicle to 90,030 feet, which became the highest altitude reached in the program. Some new and different lessons were learned through the successful flight testing of the HL-10. These lessons, when combined with information from it's sister ship, the M2-F2/F3, provided an excellent starting point for designers of future entry vehicles, including the Space Shuttle.

On current drive by Ohkawa mechanism of electron cyclotron wave in large inverse aspect ratio tokamaks

NASA Astrophysics Data System (ADS)

Zheng, Pingwei; Gong, Xueyu; Lu, Xingqiang; He, Lihua; Cao, Jingjia; Huang, Qianhong; Deng, Sheng

2018-03-01

A localized and efficient current drive method in the outer-half region of the tokamak with a large inverse aspect ratio is proposed via the Ohkawa mechanism of electron cyclotron (EC) waves. Further off-axis Ohkawa current drive (OKCD) via EC waves was investigated in high electron beta β e HL-2M-like tokamaks with a large inverse aspect ratio, and in EAST-like tokamaks with a low inverse aspect ratio. OKCD can be driven efficiently, and the driven current profile is spatially localized in the radial region, ranging from 0.62 to 0.85, where the large fraction of trapped electrons provides an excellent advantage for OKCD. Furthermore, the current drive efficiency increases with an increase in minor radius, and then drops when the minor radius beyond a certain value. The effect of trapped electrons greatly enhances the current driving capability of the OKCD mechanism. The highest current drive efficiency can reach 0.183 by adjusting the steering mirror to change the toroidal and poloidal incident angle, and the total driven current by OKCD can reach 20-32 kA MW-1 in HL-2M-like tokamaks. The current drive is less efficient for the EAST-like scenario due to the lower inverse aspect ratio. The results show that OKCD may be a valuable alternative current drive method in large inverse aspect ratio tokamaks, and the potential capabilities of OKCD can be used to suppress some important magnetohydrodynamics instabilities in the far off-axis region.

Cuphiin D1, the macrocyclic hydrolyzable tannin induced apoptosis in HL-60 cell line.

PubMed

Wang, C C; Chen, L G; Yang, L L

2000-02-28

Cuphiin D1 (CD1), a new macrocyclic hydrolyzable tannin isolated from Cuphea hyssopifolia, has been shown to exert antitumor activity both in vitro and in vivo. In this study, we explored the mechanism of the CD1-induced antitumor effect on human promyelocytic leukemia (HL-60) cells. The results showed that CD1 induced cytotoxicity in HL-60 cells and the IC50 was 16 microM after 36 h treatment. HL-60 cells treated with CD1 for 36 h decreased the uptake of [3H]-labeled thymidine, uridine and leucine in a dose dependent manner. Electron micrographs demonstrated that HL-60 cells treated with 16 microM CD1 for 36 h exhibited chromatin condensation, indicating the apoptosis occurrence. Flow cytometric analysis demonstrated the presence of apoptotic cells with low DNA content, a decrease of cell population at G2/M phase, and a concomitant increase of cell population at G1 phase. CD1 also caused DNA fragmentation and inhibited Bcl-2 expression in the HL-60 cells. These results suggest that the inhibition of Bcl-2 expression in HL-60 cell might account for the mechanism of CD1-induced apoptosis.

Minimum Fisher regularization of image reconstruction for infrared imaging bolometer on HL-2A

DOE Office of Scientific and Technical Information (OSTI.GOV)

Gao, J. M.; Liu, Y.; Li, W.

2013-09-15

An infrared imaging bolometer diagnostic has been developed recently for the HL-2A tokamak to measure the temporal and spatial distribution of plasma radiation. The three-dimensional tomography, reduced to a two-dimensional problem by the assumption of plasma radiation toroidal symmetry, has been performed. A three-dimensional geometry matrix is calculated with the one-dimensional pencil beam approximation. The solid angles viewed by the detector elements are taken into account in defining the chord brightness. And the local plasma emission is obtained by inverting the measured brightness with the minimum Fisher regularization method. A typical HL-2A plasma radiation model was chosen to optimize amore » regularization parameter on the criterion of generalized cross validation. Finally, this method was applied to HL-2A experiments, demonstrating the plasma radiated power density distribution in limiter and divertor discharges.« less

3-Hydroxy-3-methylglutaryl CoA lyase (HL): Mouse and human HL gene (HMGCL) cloning and detection of large gene deletions in two unrelated HL-deficient patients

DOE Office of Scientific and Technical Information (OSTI.GOV)

Wang, S.P.; Robert, M.F.; Mitchell, G.A.

1996-04-01

3-hydroxy-3-methylglutaryl CoA lyase (HL, EC 4.1.3.4) catalyzes the cleavage of 3-hydroxy-3-methylglutaryl CoA to acetoacetic acid and acetyl CoA, the final reaction of both ketogenesis and leucine catabolism. Autosomal-recessive HL deficiency in humans results in episodes of hypoketotic hypoglycemia and coma. Using a mouse HL cDNA as a probe, we isolated a clone containing the full-length mouse HL gene that spans about 15 kb of mouse chromosome 4 and contains nine exons. The promoter region of the mouse HL gene contains elements characteristic of a housekeeping gene: a CpG island containing multiple Sp1 binding sites surrounds exon 1, and neither amore » TATA nor a CAAT box are present. We identified multiple transcription start sites in the mouse HL gene, 35 to 9 bases upstream of the translation start codon. We also isolated two human HL genomic clones that include HL exons 2 to 9 within 18 kb. The mouse and human HL genes (HGMW-approved symbol HMGCL) are highly homologous, with identical locations of intron-exon junctions. By genomic Southern blot analysis and exonic PCR, was found 2 of 33 HL-deficient probands to be homozygous for large deletions in the HL gene. 26 refs., 4 figs., 2 tabs.« less

HL-10 on ramp

NASA Technical Reports Server (NTRS)

1966-01-01

The HL-10, seen here parked on the ramp, was one of five lifting body designs flown at NASA's Dryden Flight Research Center, Edwards, California, from July 1966 to November 1975 to study and validate the concept of safely maneuvering and landing a low lift-over-drag vehicle designed for reentry from space. Northrop Corporation built the HL-10 and M2-F2, the first two of the fleet of 'heavy' lifting bodies flown by the NASA Flight Research Center. The contract for construction of the HL-10 and the M2-F2 was $1.8 million. 'HL' stands for horizontal landing, and '10' refers to the tenth design studied by engineers at NASA's Langley Research Center, Hampton, Va. After delivery to NASA in January 1966, the HL-10 made its first flight on Dec. 22, 1966, with research pilot Bruce Peterson in the cockpit. Although an XLR-11 rocket engine was installed in the vehicle, the first 11 drop flights from the B-52 launch aircraft were powerless glide flights to assess handling qualities, stability, and control. In the end, the HL-10 was judged to be the best handling of the three original heavy-weight lifting bodies (M2-F2/F3, HL-10, X-24A). The HL-10 was flown 37 times during the lifting body research program and logged the highest altitude and fastest speed in the Lifting Body program. On Feb. 18, 1970, Air Force test pilot Peter Hoag piloted the HL-10 to Mach 1.86 (1,228 mph). Nine days later, NASA pilot Bill Dana flew the vehicle to 90,030 feet, which became the highest altitude reached in the program. Some new and different lessons were learned through the successful flight testing of the HL-10. These lessons, when combined with information from it's sister ship, the M2-F2/F3, provided an excellent starting point for designers of future entry vehicles, including the Space Shuttle.

HL-10 flight simulator

NASA Technical Reports Server (NTRS)

1968-01-01

As shown in this photo of the HL-10 flight simulator, the lifting-body pilots and engineers made use of early simulators for both training and the determination of a given vehicle's handling at various speeds, attitudes, and altitudes. This provided warning of possible problems. The HL-10 was one of five heavyweight lifting-body designs flown at NASA's Flight Research Center (FRC--later Dryden Flight Research Center), Edwards, California, from July 1966 to November 1975 to study and validate the concept of safely maneuvering and landing a low lift-over-drag vehicle designed for reentry from space. Northrop Corporation built the HL-10 and M2-F2, the first two of the fleet of 'heavy' lifting bodies flown by the NASA Flight Research Center. The contract for construction of the HL-10 and the M2-F2 was $1.8 million. 'HL' stands for horizontal landing, and '10' refers to the tenth design studied by engineers at NASA's Langley Research Center, Hampton, Va. After delivery to NASA in January 1966, the HL-10 made its first flight on Dec. 22, 1966, with research pilot Bruce Peterson in the cockpit. Although an XLR-11 rocket engine was installed in the vehicle, the first 11 drop flights from the B-52 launch aircraft were powerless glide flights to assess handling qualities, stability, and control. In the end, the HL-10 was judged to be the best handling of the three original heavy-weight lifting bodies (M2-F2/F3, HL-10, X-24A). The HL-10 was flown 37 times during the lifting body research program and logged the highest altitude and fastest speed in the Lifting Body program. On Feb. 18, 1970, Air Force test pilot Peter Hoag piloted the HL-10 to Mach 1.86 (1,228 mph). Nine days later, NASA pilot Bill Dana flew the vehicle to 90,030 feet, which became the highest altitude reached in the program. Some new and different lessons were learned through the successful flight testing of the HL-10. These lessons, when combined with information from it's sister ship, the M2-F2/F3, provided

Optics of ion beams for the neutral beam injection system on HL-2A Tokamak.

PubMed

Zou, G Q; Lei, G J; Cao, J Y; Duan, X R

2012-07-01

The ion beam optics for the neutral beam injection system on HL-2A Tokomak is studied by two- dimensional numerical simulation program firstly, where the emitting surface is taken at 100 Debye lengths from the plasma electrode. The mathematical formulation, computation techniques are described. Typical ion orbits, equipotential contours, and emittance diagram are shown. For a fixed geometry electrode, the effect of plasma density, plasma potential and plasma electron temperature on ion beam optics is examined, and the calculation reliability is confirmed by experimental results. In order to improve ion beam optics, the application of a small pre-acceleration voltage (∼100 V) between the plasma electrode and the arc discharge anode is reasonable, and a lower plasma electron temperature is desired. The results allow optimization of the ion beam optics in the neutral beam injection system on HL-2A Tokomak and provide guidelines for designing future neutral beam injection system on HL-2M Tokomak.

HL-10 on ramp

NASA Technical Reports Server (NTRS)

1966-01-01

The HL-10, seen here parked on the ramp at NASA's Flight Research Center in 1966, had a radically different shape from that of the M2-F2/F3. While the M2s were flat on top and had rounded undersides (giving them a bathtub shape), the HL-10 had a flat lower surface and a rounded top. Both shapes provided lift without wings, however. This photo was taken before the HL-10's fins were modified. The HL-10 was one of five heavyweight lifting-body designs flown at NASA's Flight Research Center (FRC--later Dryden Flight Research Center), Edwards, California, from July 1966 to November 1975 to study and validate the concept of safely maneuvering and landing a low lift-over-drag vehicle designed for reentry from space. Northrop Corporation built the HL-10 and M2-F2, the first two of the fleet of 'heavy' lifting bodies flown by the NASA Flight Research Center. The contract for construction of the HL-10 and the M2-F2 was $1.8 million. 'HL' stands for horizontal landing, and '10' refers to the tenth design studied by engineers at NASA's Langley Research Center, Hampton, Va. After delivery to NASA in January 1966, the HL-10 made its first flight on Dec. 22, 1966, with research pilot Bruce Peterson in the cockpit. The vehicle was unstable and required modifications to its fins to improve its handling qualities and stability. Although an XLR-11 rocket engine was installed in the vehicle, the first 11 drop flights from the B-52 launch aircraft were powerless glide flights to assess handling qualities, stability, and control. In the end, the HL-10 was judged to be the best handling of the three original heavy-weight lifting bodies (M2-F2/F3, HL-10, X-24A). The HL-10 was flown 37 times during the lifting body research program and logged the highest altitude and fastest speed in the Lifting Body program. On Feb. 18, 1970, Air Force test pilot Peter Hoag piloted the HL-10 to Mach 1.86 (1,228 mph). Nine days later, NASA pilot Bill Dana flew the vehicle to 90,030 feet, which became the

A digital wide range neutron flux measuring system for HL-2A

NASA Astrophysics Data System (ADS)

Yuan, Chen; Wu, Jun; Yin, Zejie

2017-08-01

To achieve wide-range, high-integration, and real-time performance on the neutron flux measurement on the HL-2A tokamak, a digital neutron flux measuring (DNFM) system based on the peripheral component interconnection (PCI) eXtension for Instrumentation express (PXIe) bus was designed. This system comprises a charge-sensitive preamplifier and a field programmable gate array (FPGA)-based main electronics plug-in. The DNFM totally covers source-range and intermediate-range neutron flux measurements, and increases system integration by a large margin through joining the pulse-counting mode and Campbell mode. Meanwhile, the neutron flux estimation method based on pulse piling proportions is able to choose and switch measuring modes in accordance with current flux, and this ensures the accuracy of measurements when the neutron flux changes suddenly. It has been demonstrated by simulated signals that the DNFM enhances the full-scale measuring range up to 1.9 × 108 cm-2 s-1, with relative error below 6.1%. The DNFM has been verified to provide a high temporal sensitivity at 10 ms time intervals on a single fission chamber on HL-2A. Contributed paper, published as part of the Proceedings of the 3rd Domestic Electromagnetic Plasma Diagnostics Workshop, September 2016, Hefei, China.

HL-10 first flight landing

NASA Technical Reports Server (NTRS)

1966-01-01

The HL-10 Lifting Body completes its first research flight with a landing on Rogers Dry Lake. Due to control problems, pilot Bruce Peterson had to land at a higher speed than originally planned in order to keep the vehicle under control. The actual touchdown speed was about 280 knots. This was 30 knots above the speed called for in the flight plan. The HL-10's first flight had lasted 3 minutes and 9 seconds. The HL-10 was one of five heavyweight lifting-body designs flown at NASA's Flight Research Center (FRC--later Dryden Flight Research Center), Edwards, California, from July 1966 to November 1975 to study and validate the concept of safely maneuvering and landing a low lift-over-drag vehicle designed for reentry from space. Northrop Corporation built the HL-10 and M2-F2, the first two of the fleet of 'heavy' lifting bodies flown by the NASA Flight Research Center. The contract for construction of the HL-10 and the M2-F2 was $1.8 million. 'HL' stands for horizontal landing, and '10' refers to the tenth design studied by engineers at NASA's Langley Research Center, Hampton, Va. After delivery to NASA in January 1966, the HL-10 made its first flight on Dec. 22, 1966, with research pilot Bruce Peterson in the cockpit. Although an XLR-11 rocket engine was installed in the vehicle, the first 11 drop flights from the B-52 launch aircraft were powerless glide flights to assess handling qualities, stability, and control. In the end, the HL-10 was judged to be the best handling of the three original heavy-weight lifting bodies (M2-F2/F3, HL-10, X-24A). The HL-10 was flown 37 times during the lifting body research program and logged the highest altitude and fastest speed in the Lifting Body program. On Feb. 18, 1970, Air Force test pilot Peter Hoag piloted the HL-10 to Mach 1.86 (1,228 mph). Nine days later, NASA pilot Bill Dana flew the vehicle to 90,030 feet, which became the highest altitude reached in the program. Some new and different lessons were learned through the

HL-10 first flight landing

NASA Technical Reports Server (NTRS)

1966-01-01

The HL-10 Lifting Body completes its first research flight with a landing on Rogers Dry Lake at Edwards AFB, California, on December 22, 1966. The HL-10 suffered from buffeting and poor control during the flight. Pilot Bruce Peterson was able to make a successful landing despite the severe problems. These were traced to airflow separation from the fins. As a result, the fins were no longer able to stabilize the vehicle. A small reshaping of the fins' leading edges cured the airflow separation, but it was not until March 15, 1968, that the second HL-10 flight occurred. The HL-10 was one of five heavyweight lifting-body designs flown at NASA's Flight Research Center (FRC--later Dryden Flight Research Center), Edwards, California, from July 1966 to November 1975 to study and validate the concept of safely maneuvering and landing a low lift-over-drag vehicle designed for reentry from space. Northrop Corporation built the HL-10 and M2-F2, the first two of the fleet of 'heavy' lifting bodies flown by the NASA Flight Research Center. The contract for construction of the HL-10 and the M2-F2 was $1.8 million. 'HL' stands for horizontal landing, and '10' refers to the tenth design studied by engineers at NASA's Langley Research Center, Hampton, Va. After delivery to NASA in January 1966, the HL-10 made its first flight on Dec. 22, 1966, with research pilot Bruce Peterson in the cockpit. Although an XLR-11 rocket engine was installed in the vehicle, the first 11 drop flights from the B-52 launch aircraft were powerless glide flights to assess handling qualities, stability, and control. In the end, the HL-10 was judged to be the best handling of the three original heavy-weight lifting bodies (M2-F2/F3, HL-10, X-24A). The HL-10 was flown 37 times during the lifting body research program and logged the highest altitude and fastest speed in the Lifting Body program. On Feb. 18, 1970, Air Force test pilot Peter Hoag piloted the HL-10 to Mach 1.86 (1,228 mph). Nine days later, NASA

Evolution of E × B shear and coherent fluctuations prior to H-L transitions in DIII-D and control strategies for H-L transitions

DOE Office of Scientific and Technical Information (OSTI.GOV)

Eldon, David; Boivin, Rejean L.; Chrystal, Colin

While operating a magnetic fusion device in H-mode has many advantages, care must be taken to understand and control the release of energy during the H-L back transition, as the extra energy stored within the H-mode transport barrier will have the potential to cause damage to material components of a large future tokamak such as ITER. Examining a scenario where the H-L back transition sequence begins before the E × B shearing layer decays on its own, we identify a long-lived precursor mode that is tied to the events of the H-L sequence and we develop a robust control strategymore » for ensuring gradual release of energy during the transition sequence. Back transitions in this scenario commonly begin with a rapid relaxation of the pedestal, which was previously shown to be inconsistent with ideal peeling-ballooning instability as the trigger, despite being otherwise similar to a large type-I Edge Localized Mode (ELM). Here, this so-called transient occurs when the E × B shearing rate ω E×B is significantly larger than the turbulence decorrelation rate ωT, indicating that this is not the result of runaway turbulence recovery. The transient is always synchronous with amplitude and propagation velocity modulations of the precursor mode, which has been dubbed the Modulating Pedestal Mode (MPM).The MPM is a coherent density fluctuation, which, in our scenario at least, reliably appears in the steep gradient region with f ≈ 70 kHz, k θ ≈ 0.3 cm –1, and it exists for ≳100 ms before the onset of back transitions. The transient may be reliably eliminated by reducing toroidal rotation in the co-current direction by the application of torque from counter-injecting neutral beams. The transient in these “soft” H-L transitions is then replaced by a small type-III ELM, which is also always synchronous with the MPM, and MPM shows the same behavior in both hard and soft cases.« less

Evolution of E × B shear and coherent fluctuations prior to H-L transitions in DIII-D and control strategies for H-L transitions

DOE PAGES

Eldon, David; Boivin, Rejean L.; Chrystal, Colin; ...

2015-11-19

While operating a magnetic fusion device in H-mode has many advantages, care must be taken to understand and control the release of energy during the H-L back transition, as the extra energy stored within the H-mode transport barrier will have the potential to cause damage to material components of a large future tokamak such as ITER. Examining a scenario where the H-L back transition sequence begins before the E × B shearing layer decays on its own, we identify a long-lived precursor mode that is tied to the events of the H-L sequence and we develop a robust control strategymore » for ensuring gradual release of energy during the transition sequence. Back transitions in this scenario commonly begin with a rapid relaxation of the pedestal, which was previously shown to be inconsistent with ideal peeling-ballooning instability as the trigger, despite being otherwise similar to a large type-I Edge Localized Mode (ELM). Here, this so-called transient occurs when the E × B shearing rate ω E×B is significantly larger than the turbulence decorrelation rate ωT, indicating that this is not the result of runaway turbulence recovery. The transient is always synchronous with amplitude and propagation velocity modulations of the precursor mode, which has been dubbed the Modulating Pedestal Mode (MPM).The MPM is a coherent density fluctuation, which, in our scenario at least, reliably appears in the steep gradient region with f ≈ 70 kHz, k θ ≈ 0.3 cm –1, and it exists for ≳100 ms before the onset of back transitions. The transient may be reliably eliminated by reducing toroidal rotation in the co-current direction by the application of torque from counter-injecting neutral beams. The transient in these “soft” H-L transitions is then replaced by a small type-III ELM, which is also always synchronous with the MPM, and MPM shows the same behavior in both hard and soft cases.« less

HL-10 on lakebed

NASA Technical Reports Server (NTRS)

1968-01-01

The HL-10 Lifting Body is seen here parked on Rogers Dry Lake, the unique location where it landed after research flights. This 1968 photo shows the vehicle after the fins were modified to remove instabilities encountered on the first flight. It involved a change to the shape of the leading edge of the fins to eliminate flow separation. It required extensive wind-tunnel testing at Langley Research Center, Hampton, Va. NASA Flight Research Center (FRC) engineer Bob Kempel than plotted thousands of data points by hand to come up with the modification, which involved a fiberglass glove backed with a metal structure on each fin's leading edge. This transformed the vehicle from a craft that was difficult to control into the best handling of the original group of lifting bodies at the FRC. The HL-10 was one of five heavyweight lifting-body designs flown at NASA's Flight Research Center (FRC--later Dryden Flight Research Center), Edwards, California, from July 1966 to November 1975 to study and validate the concept of safely maneuvering and landing a low lift-over-drag vehicle designed for reentry from space. Northrop Corporation built the HL-10 and M2-F2, the first two of the fleet of 'heavy' lifting bodies flown by the NASA Flight Research Center. The contract for construction of the HL-10 and the M2-F2 was $1.8 million. 'HL' stands for horizontal landing, and '10' refers to the tenth design studied by engineers at NASA's Langley Research Center, Hampton, Va. After delivery to NASA in January 1966, the HL-10 made its first flight on Dec. 22, 1966, with research pilot Bruce Peterson in the cockpit. Although an XLR-11 rocket engine was installed in the vehicle, the first 11 drop flights from the B-52 launch aircraft were powerless glide flights to assess handling qualities, stability, and control. In the end, the HL-10 was judged to be the best handling of the three original heavy-weight lifting bodies (M2-F2/F3, HL-10, X-24A). The HL-10 was flown 37 times during the

HL-10 in flight after launch

NASA Technical Reports Server (NTRS)

1969-01-01

The HL-10 Lifting Body is seen here in powered flight shortly after launch from the B-52 mothership. When HL-10 powered flights began on October 23, 1968, the vehicle used the same basic XLR-11 rocket engine that powered the original X-1s. A total of five powered flights were made before the HL-10 first flew supersonically on May 9, 1969, with John Manke in the pilot's seat. The HL-10 was one of five heavyweight lifting-body designs flown at NASA's Flight Research Center (FRC--later Dryden Flight Research Center), Edwards, California, from July 1966 to November 1975 to study and validate the concept of safely maneuvering and landing a low lift-over-drag vehicle designed for reentry from space. Northrop Corporation built the HL-10 and M2-F2, the first two of the fleet of 'heavy' lifting bodies flown by the NASA Flight Research Center. The contract for construction of the HL-10 and the M2-F2 was $1.8 million. 'HL' stands for horizontal landing, and '10' refers to the tenth design studied by engineers at NASA's Langley Research Center, Hampton, Va. After delivery to NASA in January 1966, the HL-10 made its first flight on Dec. 22, 1966, with research pilot Bruce Peterson in the cockpit. Although an XLR-11 rocket engine was installed in the vehicle, the first 11 drop flights from the B-52 launch aircraft were powerless glide flights to assess handling qualities, stability, and control. In the end, the HL-10 was judged to be the best handling of the three original heavy-weight lifting bodies (M2-F2/F3, HL-10, X-24A). The HL-10 was flown 37 times during the lifting body research program and logged the highest altitude and fastest speed in the Lifting Body program. On Feb. 18, 1970, Air Force test pilot Peter Hoag piloted the HL-10 to Mach 1.86 (1,228 mph). Nine days later, NASA pilot Bill Dana flew the vehicle to 90,030 feet, which became the highest altitude reached in the program. Some new and different lessons were learned through the successful flight testing of the

Pilot Peter Hoag and HL-10

NASA Technical Reports Server (NTRS)

1969-01-01

Air Force Major Peter Hoag stands in front of the HL-10 Lifting Body. Maj. Hoag joined the HL-10 program in 1969 and made his first glide flight on June 6, 1969. He made a total of 8 flights in the HL-10. They included the fastest lifting-body flight, which reached Mach 1.861 on Feb. 18, 1970. The HL-10 was one of five heavyweight lifting-body designs flown at NASA's Flight Research Center (FRC--later Dryden Flight Research Center), Edwards, California, from July 1966 to November 1975 to study and validate the concept of safely maneuvering and landing a low lift-over-drag vehicle designed for reentry from space. Northrop Corporation built the HL-10 and M2-F2, the first two of the fleet of 'heavy' lifting bodies flown by the NASA Flight Research Center. The contract for construction of the HL-10 and the M2-F2 was $1.8 million. 'HL' stands for horizontal landing, and '10' refers to the tenth design studied by engineers at NASA's Langley Research Center, Hampton, Va. After delivery to NASA in January 1966, the HL-10 made its first flight on Dec. 22, 1966, with research pilot Bruce Peterson in the cockpit. Although an XLR-11 rocket engine was installed in the vehicle, the first 11 drop flights from the B-52 launch aircraft were powerless glide flights to assess handling qualities, stability, and control. In the end, the HL-10 was judged to be the best handling of the three original heavy-weight lifting bodies (M2-F2/F3, HL-10, X-24A). The HL-10 was flown 37 times during the lifting body research program and logged the highest altitude and fastest speed in the Lifting Body program. On Feb. 18, 1970, Air Force test pilot Peter Hoag piloted the HL-10 to Mach 1.86 (1,228 mph). Nine days later, NASA pilot Bill Dana flew the vehicle to 90,030 feet, which became the highest altitude reached in the program. Some new and different lessons were learned through the successful flight testing of the HL-10. These lessons, when combined with information from it's sister ship, the M2

HL-10 in flight over lakebed

NASA Technical Reports Server (NTRS)

1969-01-01

The HL-10 lifting body is seen here in flight over Rogers Dry Lake at Edwards AFB. After the vehicle's fins were modified following its first flight, the HL-10 proved to be the best handling of the heavy-weight lifting bodies flown at Edwards Air Force Base. The HL-10 flew much better than the M2-F2, and pilots were eager to fly it. The HL-10 was one of five heavyweight lifting-body designs flown at NASA's Flight Research Center (FRC--later Dryden Flight Research Center), Edwards, California, from July 1966 to November 1975 to study and validate the concept of safely maneuvering and landing a low lift-over-drag vehicle designed for reentry from space. Northrop Corporation built the HL-10 and M2-F2, the first two of the fleet of 'heavy' lifting bodies flown by the NASA Flight Research Center. The contract for construction of the HL-10 and the M2-F2 was $1.8 million. 'HL' stands for horizontal landing, and '10' refers to the tenth design studied by engineers at NASA's Langley Research Center, Hampton, Va. After delivery to NASA in January 1966, the HL-10 made its first flight on Dec. 22, 1966, with research pilot Bruce Peterson in the cockpit. Although an XLR-11 rocket engine was installed in the vehicle, the first 11 drop flights from the B-52 launch aircraft were powerless glide flights to assess handling qualities, stability, and control. In the end, the HL-10 was judged to be the best handling of the three original heavy-weight lifting bodies (M2-F2/F3, HL-10, X-24A). The HL-10 was flown 37 times during the lifting body research program and logged the highest altitude and fastest speed in the Lifting Body program. On Feb. 18, 1970, Air Force test pilot Peter Hoag piloted the HL-10 to Mach 1.86 (1,228 mph). Nine days later, NASA pilot Bill Dana flew the vehicle to 90,030 feet, which became the highest altitude reached in the program. Some new and different lessons were learned through the successful flight testing of the HL-10. These lessons, when combined with

HL-10 in flight over lakebed

NASA Technical Reports Server (NTRS)

1969-01-01

The HL-10 Lifting Body is seen here in flight over Rogers Dry lakebed. Like the other lifting bodies, the HL-10 made a steep descent toward the lakebed, followed by a high-speed landing. This was due to the vehicle's low lift-over-drag ratio. The first 11 flights of the HL-10 were unpowered, flown to check the vehicle's handling and stability before rocket-powered flights began using the XLR-11 rocket engine. The HL-10 was one of five heavyweight lifting-body designs flown at NASA's Flight Research Center (FRC--later Dryden Flight Research Center), Edwards, California, from July 1966 to November 1975 to study and validate the concept of safely maneuvering and landing a low lift-over-drag vehicle designed for reentry from space. Northrop Corporation built the HL-10 and M2-F2, the first two of the fleet of 'heavy' lifting bodies flown by the NASA Flight Research Center. The contract for construction of the HL-10 and the M2-F2 was $1.8 million. 'HL' stands for horizontal landing, and '10' refers to the tenth design studied by engineers at NASA's Langley Research Center, Hampton, Va. After delivery to NASA in January 1966, the HL-10 made its first flight on Dec. 22, 1966, with research pilot Bruce Peterson in the cockpit. Although an XLR-11 rocket engine was installed in the vehicle, the first 11 drop flights from the B-52 launch aircraft were powerless glide flights to assess handling qualities, stability, and control. In the end, the HL-10 was judged to be the best handling of the three original heavy-weight lifting bodies (M2-F2/F3, HL-10, X-24A). The HL-10 was flown 37 times during the lifting body research program and logged the highest altitude and fastest speed in the Lifting Body program. On Feb. 18, 1970, Air Force test pilot Peter Hoag piloted the HL-10 to Mach 1.86 (1,228 mph). Nine days later, NASA pilot Bill Dana flew the vehicle to 90,030 feet, which became the highest altitude reached in the program. Some new and different lessons were learned through

Inward rectifier potassium channels in the HL-1 cardiomyocyte-derived cell line.

PubMed

Goldoni, Dana; Zhao, YouYou; Green, Brian D; McDermott, Barbara J; Collins, Anthony

2010-11-01

HL-1 is a line of immortalized cells of cardiomyocyte origin that are a useful complement to native cardiomyocytes in studies of cardiac gene regulation. Several types of ion channel have been identified in these cells, but not the physiologically important inward rectifier K(+) channels. Our aim was to identify and characterize inward rectifier K(+) channels in HL-1 cells. External Ba(2+) (100 µM) inhibited 44 ± 0.05% (mean ± s.e.m., n = 11) of inward current in whole-cell patch-clamp recordings. The reversal potential of the Ba(2+)-sensitive current shifted with external [K(+)] as expected for K(+)-selective channels. The slope conductance of the inward Ba(2+)-sensitive current increased with external [K(+)]. The apparent Kd for Ba(2+) was voltage dependent, ranging from 15 µM at -150  mV to 148 µM at -75  mV in 120  mM external K(+). This current was insensitive to 10 µM glybenclamide. A component of whole-cell current was sensitive to 150 µM 4,4'-diisothiocyanatostilbene-2,2'-disulfonic acid (DIDS), although it did not correspond to the Ba(2+)-sensitive component. The effect of external 1 mM Cs(+) was similar to that of Ba(2+). Polymerase chain reaction using HL-1 cDNA as template and primers specific for the cardiac inward rectifier K(ir)2.1 produced a fragment of the expected size that was confirmed to be K(ir)2.1 by DNA sequencing. In conclusion, HL-1 cells express a current that is characteristic of cardiac inward rectifier K(+) channels, and express K(ir)2.1 mRNA. This cell line may have use as a system for studying inward rectifier gene regulation in a cardiomyocyte phenotype. © 2010 Wiley-Liss, Inc.

Investigations on the heat flux and impurity for the HL-2M divertor

NASA Astrophysics Data System (ADS)

Zheng, G. Y.; Cai, L. Z.; Duan, X. R.; Xu, X. Q.; Ryutov, D. D.; Cai, L. J.; Liu, X.; Li, J. X.; Pan, Y. D.

2016-12-01

The controllability of the heat load and impurity in the divertor is very important, which could be one of the critical problems to be solved in order to ensure the success for a steady state tokamak. HL-2M has the advantage of the poloidal field (PF) coils placed inside the demountable toroidal field (TF) coils and close to the main plasma. As a result, it is possible to make highly accurate configuration control of the advanced divertor for HL-2M. The divertor target geometry of HL-2M has been designed to be compatible with different divertor configurations to study the divertor physics and support the high performance plasma operations. In this paper, the heat loads and impurities with different divertor configurations, including the standard X-point divertor, the snowflake-minus divertor and two tripod divertor configurations for HL-2M, are investigated by numerical simulations with the SOLPS5.0 code under the current design of the HL-2M divertor geometry. The plasmas with different conditions, such as the low discharge parameters with {{I}\\text{p}}   =  0.5 MA at the first stage of HL-2M and the high parameters with {{I}\\text{p}}   =  2.0 MA during the normal operations, are simulated. The heat load profiles and the impurity distributions are obtained, and the control of the peak heat load and the effect of impurity on the core plasma are discussed. The compatibility of different divertor configurations for HL-2M is also evaluated. It is seen that the excellent compatibility of different divertor configurations with the current divertor geometry has been verified. The results show that the snowflake-minus divertor and the tripod divertor with {{d}x}=30 \\text{cm} present good performance in terms of the heat load profiles and the impurity distributions under different conditions, which may not have a big effect on the core plasma. In addition, it is possible to optimize the distance between the two X-points, {{d}x} , to achieve a better

Investigation of the long-lived saturated internal mode and its control on the HL-2A tokamak

NASA Astrophysics Data System (ADS)

Wei, Deng; Yi, Liu; Xian-Qu, Wang; Wei, Chen; Yun-Bo, Dong; Ohdachi, S.; Xiao-Quan, Ji; Yong, Shen; Jian-Yong, Cao; Jun, Zhou; Bei-Bing, Feng; Yong-Gao, Li; Xian-Li, Huang; Jin-Ming, Gao; Xiao-Yu, Han; Mei, Huang; Xiao-Gang, Wang

2014-01-01

HL-2A plasmas heated by neutral beam injection (NBI) regularly exhibit n = 1 long-lived saturated magnetohydrodynamic instabilities. A reduction in the electron density and plasma stored energy and an increase in fast ion losses are usually observed in the presence of such perturbations. The observed long-lived saturated internal mode (LLM) occurs when the safety factor profile has a weak shear in a broad range of the plasma centre with qmin around unity. It is found that the ideal interchange mode can become marginally stable due to the weak magnetic shear reaching a critical value. The LLM, due to its pressure-driven feature, is destabilized by the strong interaction with fast ions in the low-shear region during the NBI. Furthermore, for the first time it is clearly observed that the LLMs can be suppressed by electron cyclotron resonant heating (ECRH), or by supersonic molecular beam injection in HL-2A plasmas. Low-n sidebands observed during the LLM are also suppressed by increasing the ECRH power. The control of LLMs is due to the change in the magnetic shear or in the pressure profile induced by the local heating or fuelling.

Investigation of impurity transport using laser blow-off technique in the HL-2A Ohmic and ECRH plasmas

NASA Astrophysics Data System (ADS)

Kai, Zhang; Zheng-Ying, Cui; Ping, Sun; Chun-Feng, Dong; Wei, Deng; Yun-Bo, Dong; Shao-Dong, Song; Min, Jiang; Yong-Gao, Li; Ping, Lu; Qing-Wei, Yang

2016-06-01

Impurity transports in two neighboring discharges with and without electron cyclotron resonance heating (ECRH) are studied in the HL-2A tokamak by laser blow-off (LBO) technique. The progression of aluminium ions as the trace impurity is monitored by soft x-ray (SXR) and bolometer detector arrays with good temporal and spatial resolutions. Obvious difference in the time trace of the signal between the Ohmic and ECRH L-mode discharges is observed. Based on the numerical simulation with one-dimensional (1D) impurity transport code STRAHL, the radial profiles of impurity diffusion coefficient D and convective velocity V are obtained for each shot. The result shows that the diffusion coefficient D significantly increases throughout the plasma minor radius for the ECRH case with respect to the Ohmic case, and that the convection velocity V changes from negative (inward) for the Ohmic case to partially positive (outward) for the ECRH case. The result on HL-2A confirms the pump out effect of ECRH on impurity profile as reported on various other devices.

Influence of m / n = 2/1 magnetic islands on perpendicular flows and turbulence in HL-2A Ohmic plasmas

NASA Astrophysics Data System (ADS)

Jiang, M.; Zhong, W. L.; Xu, Y.; Shi, Z. B.; Chen, W.; Ji, X. Q.; Ding, X. T.; Yang, Z. C.; Shi, P. W.; Liang, A. S.; Wen, J.; Li, J. Q.; Zhou, Y.; Li, Y. G.; Yu, D. L.; Liu, Y.; Yang, Q. W.; the HL-2A Team

2018-02-01

The radial profiles of perpendicular flows in the presence of the m/n=2/1 magnetic island were firstly measured in the HL-2A tokamak by hopping the work frequency of the Doppler backward scattering reflectometer system along with a two-dimensional electron cyclotron emission imaging diagnostic identifying the island locations. It has been observed that across the O-point cut the perpendicular flow is quite small at the center of the island and strongly enhanced around the boundary of the island, resulting in a large increase of the flow shear in the outer half island, while across the X-point cut the flow is almost flat in the whole island region. Meanwhile it was found that the density fluctuations are generally weakened inside the island. The results indicate that both the perpendicular flow and the density fluctuation level are modulated by the naturally rotating tearing mode near the island boundary. The cross-correlation between the perpendicular flows and the oscillating electron temperature further reveals that the modulation of the perpendicular flow occurs mainly inside and in the vicinity of the island.

HL-10 on lakebed with pilot Bill Dana

NASA Technical Reports Server (NTRS)

1969-01-01

This photo shows the HL-10 on Rogers Dry Lakebed with pilot Bill Dana in the foreground. Bill joined the HL-10 program in 1969 after flying the M2-F1 and the X-15, among other aircraft. His first glide flight was on April 25, 1969. Some months later, on September 3, 1969, he reached an altitude of 77,960 feet. This was one of a series of HL-10 flights to collect stability and control data at higher speeds and altitudes and at different angles of attack. The HL-10 was one of five heavyweight lifting-body designs flown at NASA's Flight Research Center (FRC--later Dryden Flight Research Center), Edwards, California, from July 1966 to November 1975 to study and validate the concept of safely maneuvering and landing a low lift-over-drag vehicle designed for reentry from space. Northrop Corporation built the HL-10 and M2-F2, the first two of the fleet of 'heavy' lifting bodies flown by the NASA Flight Research Center. The contract for construction of the HL-10 and the M2-F2 was $1.8 million. 'HL' stands for horizontal landing, and '10' refers to the tenth design studied by engineers at NASA's Langley Research Center, Hampton, Va. After delivery to NASA in January 1966, the HL-10 made its first flight on Dec. 22, 1966, with research pilot Bruce Peterson in the cockpit. Although an XLR-11 rocket engine was installed in the vehicle, the first 11 drop flights from the B-52 launch aircraft were powerless glide flights to assess handling qualities, stability, and control. In the end, the HL-10 was judged to be the best handling of the three original heavy-weight lifting bodies (M2-F2/F3, HL-10, X-24A). The HL-10 was flown 37 times during the lifting body research program and logged the highest altitude and fastest speed in the Lifting Body program. On Feb. 18, 1970, Air Force test pilot Peter Hoag piloted the HL-10 to Mach 1.86 (1,228 mph). Nine days later, NASA pilot Bill Dana flew the vehicle to 90,030 feet, which became the highest altitude reached in the program. Some new

Recent Doppler Backscattering results from EAST tokamak

NASA Astrophysics Data System (ADS)

Zhou, Chu; Liu, Adi; Zhang, Xiaohui; Hu, Jianqiang; Wang, Mingyuan; Yu, Changxuan; Liu, Wandong; Li, Hong; Lan, Tao; Sun, Xuan; Xie, Jinlin; Ding, Weixing; CAS Key Laboratory of Geospace Environment, University of Science and Technology of China Team; Department of Physics and Astronomy, University of California at Los Angeles Collaboration

2013-10-01

A Doppler reflectometer system has recently been installed in the EAST tokamak. It includes two separated systems, one for Q-band and the other for V-band. The optical system consists of a fixed flat mirror and a steerable parabolic mirror, which enabling the measurement of perpendicular wave number in the range of 4-22/cm, with the wave number resolution around 2/cm, while the radial location can cover the whole minor radius for L mode and the whole pedestal for H mode on EAST. A 2D Gaussion Ray tracing code is used to calculate the scattering location, the perpendicular wave number and the resolution. In EAST last experimental campaign the Doppler shifted signals have been obtained and the radial profiles of the perpendicular propagation velocity during L-mode and H-mode are calculated. The Er evolution during L-H and H-L transition have also been measured. The two separated systems are also used as a poloidal coherent system together to study the GAM in EAST tokamak.

HL-10 on lakebed with B-52 flyby

NASA Technical Reports Server (NTRS)

1969-01-01

NASA research pilot Bill Dana takes a moment to watch NASA's NB-52B cruise overhead after a research flight in the HL-10. On the left, John Reeves can be seen at the cockpit of the lifting body. The HL-10 was one of five heavyweight lifting-body designs flown at NASA's Flight Research Center (FRC--later Dryden Flight Research Center), Edwards, California, from July 1966 to November 1975 to study and validate the concept of safely maneuvering and landing a low lift-over-drag vehicle designed for reentry from space. Northrop Corporation built the HL-10 and M2-F2, the first two of the fleet of 'heavy' lifting bodies flown by the NASA Flight Research Center. The contract for construction of the HL-10 and the M2-F2 was $1.8 million. 'HL' stands for horizontal landing, and '10' refers to the tenth design studied by engineers at NASA's Langley Research Center, Hampton, Va. After delivery to NASA in January 1966, the HL-10 made its first flight on Dec. 22, 1966, with research pilot Bruce Peterson in the cockpit. Although an XLR-11 rocket engine was installed in the vehicle, the first 11 drop flights from the B-52 launch aircraft were powerless glide flights to assess handling qualities, stability, and control. In the end, the HL-10 was judged to be the best handling of the three original heavy-weight lifting bodies (M2-F2/F3, HL-10, X-24A). The HL-10 was flown 37 times during the lifting body research program and logged the highest altitude and fastest speed in the Lifting Body program. On Feb. 18, 1970, Air Force test pilot Peter Hoag piloted the HL-10 to Mach 1.86 (1,228 mph). Nine days later, NASA pilot Bill Dana flew the vehicle to 90,030 feet, which became the highest altitude reached in the program. Some new and different lessons were learned through the successful flight testing of the HL-10. These lessons, when combined with information from it's sister ship, the M2-F2/F3, provided an excellent starting point for designers of future entry vehicles, including the

[HL7 standard--features, principles, and methodology].

PubMed

Koncar, Miroslav

2005-01-01

The mission of HL7 Inc. non-profit organization is to provide standards for the exchange, management and integration of data that support clinical patient care, and the management, delivery and evaluation of healthcare services. As the standards developed by HL7 Inc. represent the world's most influential standardization efforts in the field of medical informatics, the HL7 family of standards has been recognized by the technical and scientific community as the foundation for the next generation healthcare information systems. Versions 1 and 2 of HL7 standard have solved many issues, but also demonstrated the size and complexity of health information sharing problem. As the solution complete new methodology has been adopted that is encompassed in the HL7 Version 3 recommendations. This approach standardizes Reference Information Model (RIM), which is the source of all derived domain models and message structures. Message design is now defined in detail, enabling interoperability between loosely coupled systems that are.designed by different vendors and deployed in various environments. At the start of the Primary Healthcare Information System project in the Republic of Croatia in 2002, the decision was to go directly to Version 3. The target scope of work includes clinical, financial and administrative data management in the domain of healthcare processes. By using HL7v3 standardized methodology we were able to completely map the Croatian primary healthcare domain to HL7v3 artefacts. Further refinement processes that are planned for the future will provide semantic interoperability and detailed description of all elements in HL7 messages. Our HL7 Business Component is in constant process of studying different legacy applications, making solid foundation for their integration to HL7-enabled communication environment.

HL-10 on lakebed with pilot John Manke

NASA Technical Reports Server (NTRS)

1969-01-01

John Manke is shown here on the lakebed next to the HL-10, one of four different lifting-body vehicles he flew, including the X-24B, which he flew 16 times. His total of 42 lifting-body flights was second only to the 51 flights Milt Thompson achieved, including one in the remotely piloted Hyper III. The HL-10 was one of five heavyweight lifting-body designs flown at NASA's Flight Research Center (FRC--later Dryden Flight Research Center), Edwards, California, from July 1966 to November 1975 to study and validate the concept of safely maneuvering and landing a low lift-over-drag vehicle designed for reentry from space. Northrop Corporation built the HL-10 and M2-F2, the first two of the fleet of 'heavy' lifting bodies flown by the NASA Flight Research Center. The contract for construction of the HL-10 and the M2-F2 was $1.8 million. 'HL' stands for horizontal landing, and '10' refers to the tenth design studied by engineers at NASA's Langley Research Center, Hampton, Va. After delivery to NASA in January 1966, the HL-10 made its first flight on Dec. 22, 1966, with research pilot Bruce Peterson in the cockpit. Although an XLR-11 rocket engine was installed in the vehicle, the first 11 drop flights from the B-52 launch aircraft were powerless glide flights to assess handling qualities, stability, and control. In the end, the HL-10 was judged to be the best handling of the three original heavy-weight lifting bodies (M2-F2/F3, HL-10, X-24A). The HL-10 was flown 37 times during the lifting body research program and logged the highest altitude and fastest speed in the Lifting Body program. On Feb. 18, 1970, Air Force test pilot Peter Hoag piloted the HL-10 to Mach 1.86 (1,228 mph). Nine days later, NASA pilot Bill Dana flew the vehicle to 90,030 feet, which became the highest altitude reached in the program. Some new and different lessons were learned through the successful flight testing of the HL-10. These lessons, when combined with information from it's sister ship

Numerical investigation of disruption characteristics for the snowflake divertor configuration in HL-2M

NASA Astrophysics Data System (ADS)

Xue, L.; Duan, X. R.; Zheng, G. Y.; Liu, Y. Q.; Pan, Y. D.; Yan, S. L.; Dokuka, V. N.; Lukash, V. E.; Khayrutdinov, R. R.

2016-05-01

Cold and hot vertical displacement events (VDEs) are frequently related to the disruption of vertically-elongated tokamaks. The weak poloidal magnetic field around the null-points of a snowflake divertor configuration may influence the vertical displacement process. In this paper, the major disruption with a cold VDE and the vertical disruption in the HL-2M tokamak are investigated by the DINA code. In order to better illustrate the effect from the weak poloidal field, a double-null snowflake configuration is compared with the standard divertor (SD) configuration under the same plasma parameters. Computational results show that the weak poloidal magnetic field can be partly beneficial for mitigating the vertical instability of the plasma under small perturbations. For major disruption, the peak poloidal halo current fraction is almost the same between the snowflake and the SD configurations. However, this fraction becomes much larger for the snowflake in the event of a hot VDE. Furthermore, during the disruption for a snowflake configuration, the distribution of electromagnetic force on a vacuum vessel gets more non-uniform during the current quench.

Ranolazine inhibits shear sensitivity of endogenous Na+ current and spontaneous action potentials in HL-1 cells

PubMed Central

Strege, Peter; Beyder, Arthur; Bernard, Cheryl; Crespo-Diaz, Ruben; Behfar, Atta; Terzic, Andre; Ackerman, Michael; Farrugia, Gianrico

2012-01-01

NaV1.5 is a mechanosensitive voltage-gated Na+ channel encoded by the gene SCN5A, expressed in cardiac myocytes and required for phase 0 of the cardiac action potential (AP). In the cardiomyocyte, ranolazine inhibits depolarizing Na+ current and delayed rectifier (IKr) currents. Recently, ranolazine was also shown to be an inhibitor of NaV1.5 mechanosensitivity. Stretch also accelerates the firing frequency of the SA node, and fluid shear stress increases the beating rate of cultured cardiomyocytes in vitro. However, no cultured cell platform exists currently for examination of spontaneous electrical activity in response to mechanical stimulation. In the present study, flow of solution over atrial myocyte-derived HL-1 cultured cells was used to study shear stress mechanosensitivity of Na+ current and spontaneous, endogenous rhythmic action potentials. In voltage-clamped HL-1 cells, bath flow increased peak Na+ current by 14 ± 5%. In current-clamped cells, bath flow increased the frequency and decay rate of AP by 27 ± 12% and 18 ± 4%, respectively. Ranolazine blocked both responses to shear stress. This study suggests that cultured HL-1 cells are a viable in vitro model for detailed study of the effects of mechanical stimulation on spontaneous cardiac action potentials. Inhibition of the frequency and decay rate of action potentials in HL-1 cells are potential mechanisms behind the antiarrhythmic effect of ranolazine. PMID:23018927

Borneol Dehydrogenase from Pseudomonas sp. Strain TCU-HL1 Catalyzes the Oxidation of (+)-Borneol and Its Isomers to Camphor

PubMed Central

Tsang, Hoi-Lung; Huang, Jui-Lin; Lin, Yu-Hsuan; Huang, Kai-Fa; Lu, Pei-Luen; Lin, Guang-Huey; Khine, Aye Aye; Hu, Anren

2016-01-01

ABSTRACT Most plant-produced monoterpenes can be degraded by soil microorganisms. Borneol is a plant terpene that is widely used in traditional Chinese medicine. Neither microbial borneol dehydrogenase (BDH) nor a microbial borneol degradation pathway has been reported previously. One borneol-degrading strain, Pseudomonas sp. strain TCU-HL1, was isolated by our group. Its genome was sequenced and annotated. The genome of TCU-HL1 consists of a 6.2-Mbp circular chromosome and one circular plasmid, pTHL1 (12.6 kbp). Our results suggest that borneol is first converted into camphor by BDH in TCU-HL1 and is further decomposed through a camphor degradation pathway. The recombinant BDH was produced in the form of inclusion bodies. The apparent Km values of refolded recombinant BDH for (+)-borneol and (−)-borneol were 0.20 ± 0.01 and 0.16 ± 0.01 mM, respectively, and the kcat values for (+)-borneol and (−)-borneol were 0.75 ± 0.01 and 0.53 ± 0.01 s−1, respectively. Two plant BDH genes have been reported previously. The kcat and kcat/Km values of lavender BDH are about 1,800-fold and 500-fold lower, respectively, than those of TCU-HL1 BDH. IMPORTANCE The degradation of borneol in a soil microorganism through a camphor degradation pathway is reported in this study. We also report a microbial borneol dehydrogenase. The kcat and kcat/Km values of lavender BDH are about 1,800-fold and 500-fold lower, respectively, than those of TCU-HL1 BDH. The indigenous borneol- and camphor-degrading strain isolated, Pseudomonas sp. strain TCU-HL1, reminds us of the time 100 years ago when Taiwan was the major producer of natural camphor in the world. PMID:27542933

STORE-OPERATED CALCIUM ENTRY IS PRESENT IN HL-1 CARDIOMYOCYTES AND CONTRIBUTES TO RESTING CALCIUM

PubMed Central

Touchberry, Chad D.; Elmore, Chris J.; Nguyen, Tien M.; Andresen, Jon J.; Zhao, Xiaoli; Orange, Matthew; Weisleder, Noah; Brotto, Marco; Claycomb, William C.; Wacker, Michael J.

2011-01-01

Store-operated Ca2+ entry (SOCE) has recently been shown to be of physiological and pathological importance in the heart, particularly during cardiac hypertrophy. However, measuring changes in intracellular Ca2+ during SOCE is very difficult to study in adult primary cardiomyocytes. As a result there is a need for a stable and reliable in vitro model of SOCE which can be used to test cardiac drugs and investigate the role of SOCE in cardiac pathology. HL-1 cells are the only immortal cardiomyocyte cell line available that continuously divides and spontaneously contracts while maintaining phenotypic characteristics of the adult cardiomyocyte. To date the role of SOCE has not yet been investigated in the HL-1 cardiac cell line. We report for the first time that these cells express stromal interaction molecule 1 (STIM1) and the Ca2+ release-activated Ca2+ (CRAC) channel Orai1, which are essential components of the SOCE machinery. In addition, SOCE is tightly coupled to sarcoplasmic reticulum (SR)-Ca2+ release in HL-1 cells, and such response was not impaired in the presence of voltage dependent Ca2+ channels (L-type and T-type channels) or reverse mode Na+/ Ca2+ exchanger (NCX) inhibitors. We were able to abolish the SOCE response with known SOCE inhibitors (BTP-2 and SKF-96365) and by targeted knockdown of Orai1 with RNAi. In addition, knockdown of Orai1 resulted in lower baseline Ca2+ and an attenuated response to thapsigargin (TG) and caffeine, indicating that SOCE may play a role in Ca2+ homeostasis during unstressed conditions in cardiomyocytes. Currently, there is little knowledge about SOCE in cardiomyocytes, and the present results suggest that HL-1 cells will be of great utility in investigating the role of SOCE in the heart. PMID:22079292

Comparative analysis of the bioenergetics of adult cardiomyocytes and nonbeating HL-1 cells: respiratory chain activities, glycolytic enzyme profiles, and metabolic fluxes.

PubMed

Monge, Claire; Beraud, Nathalie; Tepp, Kersti; Pelloux, Sophie; Chahboun, Siham; Kaambre, Tuuli; Kadaja, Lumme; Roosimaa, Mart; Piirsoo, Andres; Tourneur, Yves; Kuznetsov, Andrey V; Saks, Valdur; Seppet, Enn

2009-04-01

Comparative analysis of the bioenergetic parameters of adult rat cardiomyocytes (CM) and HL-1 cells with very different structure but similar cardiac phenotype was carried out with the aim of revealing the importance of the cell structure for regulation of its energy fluxes. Confocal microscopic analysis showed very different mitochondrial arrangement in these cells. The cytochrome content per milligram of cell protein was decreased in HL-1 cells by a factor of 7 compared with CM. In parallel, the respiratory chain complex activities were decreased by 4-8 times in the HL-1 cells. On the contrary, the activities of glycolytic enzymes, hexokinase (HK), and pyruvate kinase (PK) were increased in HL-1 cells, and these cells effectively transformed glucose into lactate. At the same time, the creatine kinase (CK) activity was significantly decreased in HL-1 cells. In conclusion, the results of this study comply with the assumption that in contrast to CM in which oxidative phosphorylation is a predominant provider of ATP and the CK system is a main carrier of energy from mitochondria to ATPases, in HL-1 cells the energy metabolism is based mostly on the glycolytic reactions coupled to oxidative phosphorylation through HK.

HL-10 on lakebed with pilot Bill Dana

NASA Technical Reports Server (NTRS)

1966-01-01

NASA research pilot Bill Dana stands in front of the HL-10 Lifting Body following his first glide flight on April 25, 1969. Dana later retired Chief Engineer at NASA's Dryden Flight Research Center, which was called only the NASA Flight Research Center in 1969. Prior to his lifting body assignment, Dana flew the famed X-15 research airplane. He flew the rocket-powered aircraft 16 times, reaching a top speed of 3,897 miles per hour and a peak altitude of 310,000 feet (almost 59 miles high). The HL-10 was one of five heavyweight lifting-body designs flown at NASA's Flight Research Center (FRC--later Dryden Flight Research Center), Edwards, California, from July 1966 to November 1975 to study and validate the concept of safely maneuvering and landing a low lift-over-drag vehicle designed for reentry from space. Northrop Corporation built the HL-10 and M2-F2, the first two of the fleet of 'heavy' lifting bodies flown by the NASA Flight Research Center. The contract for construction of the HL-10 and the M2-F2 was $1.8 million. 'HL' stands for horizontal landing, and '10' refers to the tenth design studied by engineers at NASA's Langley Research Center, Hampton, Va. After delivery to NASA in January 1966, the HL-10 made its first flight on Dec. 22, 1966, with research pilot Bruce Peterson in the cockpit. Although an XLR-11 rocket engine was installed in the vehicle, the first 11 drop flights from the B-52 launch aircraft were powerless glide flights to assess handling qualities, stability, and control. In the end, the HL-10 was judged to be the best handling of the three original heavy-weight lifting bodies (M2-F2/F3, HL-10, X-24A). The HL-10 was flown 37 times during the lifting body research program and logged the highest altitude and fastest speed in the Lifting Body program. On Feb. 18, 1970, Air Force test pilot Peter Hoag piloted the HL-10 to Mach 1.86 (1,228 mph). Nine days later, NASA pilot Bill Dana flew the vehicle to 90,030 feet, which became the highest

Retinoic acid induces nuclear accumulation of Raf1 during differentiation of HL-60 cells

DOE Office of Scientific and Technical Information (OSTI.GOV)

Smith, James; Bunaciu, Rodica P.; Reiterer, Gudrun

All trans-retinoic acid (RA) is a standard therapeutic agent used in differentiation induction therapy treatment of acute promyelocytic leukemia (APL). RA and its metabolites use a diverse set of signal transduction pathways during the differentiation program. In addition to the direct transcriptional targets of the nuclear RAR and RXR receptors, signals derived from membrane receptors and the Raf-MEK-ERK pathway are required. Raf1 phosphorylation and the prolonged activation of Raf1 persisting during the entire differentiation process are required for RA-dependent differentiation of HL-60 cells. Here we identify a nuclear redistribution of Raf1 during the RA-induced differentiation of HL-60 cells. In addition,more » the nuclear accumulation of Raf1 correlates with an increase in Raf1 phosphorylated at serine 621. The serine 621 phosphorylated Raf1 is predominantly localized in the nucleus. The RA-dependent nuclear accumulation of Raf1 suggests a novel nuclear role for Raf1 during the differentiation process.« less

HL-10 after first flight with pilot Bruce Peterson

NASA Technical Reports Server (NTRS)

1966-01-01

The HL-10 after its first flight, shown with pilot Bruce Peterson. Although the lifting-body aircraft was predicted to have good flying qualities, this first flight showed major control and stability problems. The cause was airflow separation from the vehicle's fins. Changes to the fins' leading-edge shape fixed the problem. The HL-10 was one of five heavyweight lifting-body designs flown at NASA's Flight Research Center (FRC--later Dryden Flight Research Center), Edwards, California, from July 1966 to November 1975 to study and validate the concept of safely maneuvering and landing a low lift-over-drag vehicle designed for reentry from space. Northrop Corporation built the HL-10 and M2-F2, the first two of the fleet of 'heavy' lifting bodies flown by the NASA Flight Research Center. The contract for construction of the HL-10 and the M2-F2 was $1.8 million. 'HL' stands for horizontal landing, and '10' refers to the tenth design studied by engineers at NASA's Langley Research Center, Hampton, Va. After delivery to NASA in January 1966, the HL-10 made its first flight on Dec. 22, 1966, with research pilot Bruce Peterson in the cockpit. Although an XLR-11 rocket engine was installed in the vehicle, the first 11 drop flights from the B-52 launch aircraft were powerless glide flights to assess handling qualities, stability, and control. In the end, the HL-10 was judged to be the best handling of the three original heavy-weight lifting bodies (M2-F2/F3, HL-10, X-24A). The HL-10 was flown 37 times during the lifting body research program and logged the highest altitude and fastest speed in the Lifting Body program. On Feb. 18, 1970, Air Force test pilot Peter Hoag piloted the HL-10 to Mach 1.86 (1,228 mph). Nine days later, NASA pilot Bill Dana flew the vehicle to 90,030 feet, which became the highest altitude reached in the program. Some new and different lessons were learned through the successful flight testing of the HL-10. These lessons, when combined with

Overexpression of the Large-Conductance, Ca2+-Activated K+ (BK) Channel Shortens Action Potential Duration in HL-1 Cardiomyocytes.

PubMed

Stimers, Joseph R; Song, Li; Rusch, Nancy J; Rhee, Sung W

2015-01-01

Long QT syndrome is characterized by a prolongation of the interval between the Q wave and the T wave on the electrocardiogram. This abnormality reflects a prolongation of the ventricular action potential caused by a number of genetic mutations or a variety of drugs. Since effective treatments are unavailable, we explored the possibility of using cardiac expression of the large-conductance, Ca2+-activated K+ (BK) channel to shorten action potential duration (APD). We hypothesized that expression of the pore-forming α subunit of human BK channels (hBKα) in HL-1 cells would shorten action potential duration in this mouse atrial cell line. Expression of hBKα had minimal effects on expression levels of other ion channels with the exception of a small but significant reduction in Kv11.1. Patch-clamped hBKα expressing HL-1 cells exhibited an outward voltage- and Ca2+-sensitive K+ current, which was inhibited by the BK channel blocker iberiotoxin (100 nM). This BK current phenotype was not detected in untransfected HL-1 cells or in HL-1 null cells sham-transfected with an empty vector. Importantly, APD in hBKα-expressing HL-1 cells averaged 14.3 ± 2.8 ms (n = 10), which represented a 53% reduction in APD compared to HL-1 null cells lacking BKα expression. APD in the latter cells averaged 31.0 ± 5.1 ms (n = 13). The shortened APD in hBKα-expressing cells was restored to normal duration by 100 nM iberiotoxin, suggesting that a repolarizing K+ current attributed to BK channels accounted for action potential shortening. These findings provide initial proof-of-concept that the introduction of hBKα channels into a cardiac cell line can shorten APD, and raise the possibility that gene-based interventions to increase hBKα channels in cardiac cells may hold promise as a therapeutic strategy for long QT syndrome.

Recent Results of IRAN-T1 Tokamak

DOE Office of Scientific and Technical Information (OSTI.GOV)

Dorranian, D.; Ghoranneviss, M.; Salem, M. K.

2006-12-04

In this article after introducing the IR-T1 tokamak and its diagnostic systems a brief discussion on the range of grossly stable operating conditions of its plasma by Hugill diagram is presented. Hard disruption instability is studied experimentally in the next part, which confirms that MHD behavior in small tokamaks can be characterized by a single parameter q(a), safety factor at plasma edge. Finally the characteristics of the new regime of IR-T1 are reported. By our new model of triggering different fields (toroidal, ohmic and vertical), the plasma duration time is increased up to 35 ms with Ip of about 25more » kA. By modifying capacitance and charging voltage of ohmic and vertical fields the spike oscillations which was appeared in the plasma behavior is taken out. The role of cleaning the vacuum chamber and using heavier gas for glow discharge and the effect of base pressure is described in detail.« less

HL-A27 and anterior uveitis.

PubMed

Woodrow, J C; Mapstone, R; Anderson, J; Usher, N

1975-09-01

HL-A types were determined in 90 successive patients with non-granulomatous uveitis. Fifty-one were HL-A27 positive (55.7%) compared to 8.2% of controls. Of 16 patients with ankylosing spondylitis, 13 were HL-A27 positive, as were two patients with a history of Reiter's syndrome. Twenty-eight patients were HL-A27 positive but had no evidence of rheumatic disease. The findings are discussed in relation to the possible pathogenesis of uveitis.

2-(3-Methoxyphenyl)-5-methyl-1,8-naphthyridin-4(1H)-one (HKL-1) induces G2/M arrest and mitotic catastrophe in human leukemia HL-60 cells

DOE Office of Scientific and Technical Information (OSTI.GOV)

Hsu, Mei-Hua; Liu, Chin-Yu; Lin, Chiao-Min

2012-03-01

2-(3-Methoxyphenyl)-5-methyl-1,8-naphthyridin-4(1H)-one (HKL-1), a 2-phenyl-1,8-naphthyridin-4-one (2-PN) derivative, was synthesized and evaluated as an effective antimitotic agent in our laboratory. However, the molecular mechanisms are uncertain. In this study, HKL-1 was demonstrated to induce multipolar spindles, sustain mitotic arrest and generate multinucleated cells, all of which indicate mitotic catastrophe, in human leukemia HL-60 cells. Western blotting showed that HKL-1 induces mitotic catastrophe in HL-60 cells through regulating mitotic phase-specific kinases (down-regulating CDK1, cyclin B1, CENP-E, and aurora B) and regulating the expression of Bcl-2 family proteins (down-regulating Bcl-2 and up-regulating Bax and Bak), followed by caspase-9/-3 cleavage. These findings suggest that HKL-1more » appears to exert its cytotoxicity toward HL-60 cells in culture by inducing mitotic catastrophe. Highlights: ► HKL-1 is a potential antimitotic agent against HL-60 cells. ► HKL-1 induces spindle disruption and sustained resulted in mitotic catastrophe. ► CENP-E and aurora B protein expressions significantly reduced. ► Bcl-2 family protein expressions altered and caspase-9/-3 activation. ► HKL-1 is an attractive candidate for possible use as a novel antimitotic agent.« less

HL-10 on lakebed with pilot Major Jerauld R. Gentry

NASA Technical Reports Server (NTRS)

1968-01-01

Pilot Major Jerauld R. Gentry stands in front of the HL-10 Lifting Body. Gentry was the Air Force project pilot for the HL-10 while it was making the early glide and powered flights in 1968 following its modification. He made a total of nine flights in the vehicle. For his work on the HL-10, Gentry was awarded the Harmon International Trophy for his outstanding contribution to the science of flying. He later became the Air Force pilot for the X-24A. The HL-10 was one of five heavyweight lifting-body designs flown at NASA's Flight Research Center (FRC--later Dryden Flight Research Center), Edwards, California, from July 1966 to November 1975 to study and validate the concept of safely maneuvering and landing a low lift-over-drag vehicle designed for reentry from space. Northrop Corporation built the HL-10 and M2-F2, the first two of the fleet of 'heavy' lifting bodies flown by the NASA Flight Research Center. The contract for construction of the HL-10 and the M2-F2 was $1.8 million. 'HL' stands for horizontal landing, and '10' refers to the tenth design studied by engineers at NASA's Langley Research Center, Hampton, Va. After delivery to NASA in January 1966, the HL-10 made its first flight on Dec. 22, 1966, with research pilot Bruce Peterson in the cockpit. Although an XLR-11 rocket engine was installed in the vehicle, the first 11 drop flights from the B-52 launch aircraft were powerless glide flights to assess handling qualities, stability, and control. In the end, the HL-10 was judged to be the best handling of the three original heavy-weight lifting bodies (M2-F2/F3, HL-10, X-24A). The HL-10 was flown 37 times during the lifting body research program and logged the highest altitude and fastest speed in the Lifting Body program. On Feb. 18, 1970, Air Force test pilot Peter Hoag piloted the HL-10 to Mach 1.86 (1,228 mph). Nine days later, NASA pilot Bill Dana flew the vehicle to 90,030 feet, which became the highest altitude reached in the program. Some new and

HL-10 on lakebed showing subsonic control surface configuration

NASA Technical Reports Server (NTRS)

1966-01-01

This photo shows the HL-10 on lakebed with its subsonic control surface configuration. The unusual shapes of the lifting bodies, as well as the demands of flying a re-entry shape to comparative low-speed landings, required a complex set of control surfaces. The rudders also served as speed brakes, allowing the pilot to adjust his speed during descent. Moving the flaps at the rear of the fuselage in the same direction pitched the nose up, while moving them in opposite directions rolled the vehicle to the right or left. After the HL-10's fins were modified to improve its handling qualities, the vehicle proved to be the best handling of the original heavy-weight lifting bodies. The HL-10 was one of five heavyweight lifting-body designs flown at NASA's Flight Research Center (FRC--later Dryden Flight Research Center), Edwards, California, from July 1966 to November 1975 to study and validate the concept of safely maneuvering and landing a low lift-over-drag vehicle designed for reentry from space. Northrop Corporation built the HL-10 and M2-F2, the first two of the fleet of 'heavy' lifting bodies flown by the NASA Flight Research Center. The contract for construction of the HL-10 and the M2-F2 was $1.8 million. 'HL' stands for horizontal landing, and '10' refers to the tenth design studied by engineers at NASA's Langley Research Center, Hampton, Va. After delivery to NASA in January 1966, the HL-10 made its first flight on Dec. 22, 1966, with research pilot Bruce Peterson in the cockpit. Although an XLR-11 rocket engine was installed in the vehicle, the first 11 drop flights from the B-52 launch aircraft were powerless glide flights to assess handling qualities, stability, and control. In the end, the HL-10 was judged to be the best handling of the three original heavy-weight lifting bodies (M2-F2/F3, HL-10, X-24A). The HL-10 was flown 37 times during the lifting body research program and logged the highest altitude and fastest speed in the Lifting Body program. On Feb

Simplifying HL7 Version 3 messages.

PubMed

Worden, Robert; Scott, Philip

2011-01-01

HL7 Version 3 offers a semantically robust method for healthcare interoperability but has been criticized as overly complex to implement. This paper reviews initiatives to simplify HL7 Version 3 messaging and presents a novel approach based on semantic mapping. Based on user-defined definitions, precise transforms between simple and full messages are automatically generated. Systems can be interfaced with the simple messages and achieve interoperability with full Version 3 messages through the transforms. This reduces the costs of HL7 interfacing and will encourage better uptake of HL7 Version 3 and CDA.

HL-10 landing on lakebed with F-104 chase aircraft

NASA Technical Reports Server (NTRS)

1970-01-01

In this photo, the HL-10 has touched down on its main landing gear, while the pilot was holding the nose up to slow the vehicle. The F-104 in the background was used as a chase plane. Its pilot would give the HL-10's pilot calls on his altitude above the lakebed as well as warnings about any problems. The NASA F-104s were also used for lifting-body training. With the landing gear extended and flaps lowered, the F-104 could simulate the steep, high-speed descent and landing of a lifting body. The HL-10 was one of five heavyweight lifting-body designs flown at NASA's Flight Research Center (FRC--later Dryden Flight Research Center), Edwards, California, from July 1966 to November 1975 to study and validate the concept of safely maneuvering and landing a low lift-over-drag vehicle designed for reentry from space. Northrop Corporation built the HL-10 and M2-F2, the first two of the fleet of 'heavy' lifting bodies flown by the NASA Flight Research Center. The contract for construction of the HL-10 and the M2-F2 was $1.8 million. 'HL' stands for horizontal landing, and '10' refers to the tenth design studied by engineers at NASA's Langley Research Center, Hampton, Va. After delivery to NASA in January 1966, the HL-10 made its first flight on Dec. 22, 1966, with research pilot Bruce Peterson in the cockpit. Although an XLR-11 rocket engine was installed in the vehicle, the first 11 drop flights from the B-52 launch aircraft were powerless glide flights to assess handling qualities, stability, and control. In the end, the HL-10 was judged to be the best handling of the three original heavy-weight lifting bodies (M2-F2/F3, HL-10, X-24A). The HL-10 was flown 37 times during the lifting body research program and logged the highest altitude and fastest speed in the Lifting Body program. On Feb. 18, 1970, Air Force test pilot Peter Hoag piloted the HL-10 to Mach 1.86 (1,228 mph). Nine days later, NASA pilot Bill Dana flew the vehicle to 90,030 feet, which became the highest

HL-10 after landing with pilot Bill Dana

NASA Technical Reports Server (NTRS)

1960-01-01

This movie clip, running about 56 seconds, shows NASA pilot Bill Dana exiting the cockpit of the HL-10 and waving to his B-52 drop aircraft, just after landing on the dry lakebed at Edwards Air Force Base, California. A fleet of lifting bodies flown at the NASA Flight Research Center, Edwards, California, from 1963 to l975 demonstrated the ability of pilots to maneuver (in the atmosphere) and safely land a wingless vehicle. These lifting bodies were basically designed so they could fly back to Earth from space and be landed like an aircraft at a pre-determined site. (In 1976 NASA renamed the FRC as the NASA Dryden Flight Research Center in honor of Hugh L. Dryden.) These unique research vehicles, with their unconventional aerodynamic shapes, were the M2-F1, M2-F2, M2-F3, HL-10, X-24A, and the X-24B. The information the lifting body program generated contributed to the database that led to development of the current space shuttle program as well as the X-33 and X-38 technology demonstrators. Aerodynamic lift -- essential to flight in the atmosphere -- was obtained from the shape of the vehicles rather than from wings on a normal aircraft. The addition of fins and control surfaces allowed the pilots to stabilize and control the vehicles and regulate their flight paths. All but the M2-F1 were powered by the same type of XLR-11 rocket engine used in the famed Bell X-1 -- first aircraft to fly faster than the speed of sound. The M2-F1, a lightweight prototype, was unpowered. The success of the Dryden M2-F1 program led to the NASA development and construction of two heavyweight lifting bodies based on studies at NASA Ames and Langley research centers -- the M2-F2 and the HL-10, both built by the Northrop Corporation. The 'M' refers to 'manned' and 'F' refers to 'flight' version. 'HL' comes from 'horizontal landing' and '10' is for the tenth lifting body model to be investigated by Langley Research Center, Hampton, Virginia. The HL-10 was delivered to the FRC by Northrop

Study on Matching a 300 MVA Motor Generator with an Ohmic Heating Power Supply in HL-2M

NASA Astrophysics Data System (ADS)

Peng, Jianfei; Xuan, Weimin; Wang, Haibing; Li, Huajun; Wang, Yingqiao; Wang, Shujin

2013-03-01

A new 300 MVA/1350 MJ motor generator (MG) will be built to feed all of the poloidal field power supplies (PFPS) and auxiliary heating power supplies of the HL-2M tokamak. The MG has a vertical-shaft salient pole 6-phase synchronous generator and a coaxial 8500 kW induction motor. The Ohmic heating power supply (OHPS) consisting of 4-quadrant DC pulsed convertor is the one with the highest parameters among the PFPS. Therefore, the match between the generator and the OHPS is very important. The matching study with Matlab/Simulink is described in this paper. The simulation results show that the subtransient reactance of the generator is closely related to the inversion operation of the OHPS. By setting various subtransient reactance in the simulation generator model and considering the cost reduction, the optimized parameters are obtained as x″d = 0.405 p.u. at 100 Hz for the generator. The models built in the simulation can be used as an important tool for studying the dynamic characteristics and the control strategy of other HL-2M PFPSes.

HL-10 in flight, turning to line up with lakebed runway 18

NASA Technical Reports Server (NTRS)

1969-01-01

This photo shows the HL-10 in flight, turning to line up with lakebed runway 18. The pilot for this flight, the 29th of the HL-10 series, was Bill Dana. The HL-10 reached a peak altitude of 64,590 feet and a top speed of Mach 1.59 on this particular flight. The HL-10 was one of five heavyweight lifting-body designs flown at NASA's Flight Research Center (FRC--later Dryden Flight Research Center), Edwards, California, from July 1966 to November 1975 to study and validate the concept of safely maneuvering and landing a low lift-over-drag vehicle designed for reentry from space. Northrop Corporation built the HL-10 and M2-F2, the first two of the fleet of 'heavy' lifting bodies flown by the NASA Flight Research Center. The contract for construction of the HL-10 and the M2-F2 was $1.8 million. 'HL' stands for horizontal landing, and '10' refers to the tenth design studied by engineers at NASA's Langley Research Center, Hampton, Va. After delivery to NASA in January 1966, the HL-10 made its first flight on Dec. 22, 1966, with research pilot Bruce Peterson in the cockpit. Although an XLR-11 rocket engine was installed in the vehicle, the first 11 drop flights from the B-52 launch aircraft were powerless glide flights to assess handling qualities, stability, and control. In the end, the HL-10 was judged to be the best handling of the three original heavy-weight lifting bodies (M2-F2/F3, HL-10, X-24A). The HL-10 was flown 37 times during the lifting body research program and logged the highest altitude and fastest speed in the Lifting Body program. On Feb. 18, 1970, Air Force test pilot Peter Hoag piloted the HL-10 to Mach 1.86 (1,228 mph). Nine days later, NASA pilot Bill Dana flew the vehicle to 90,030 feet, which became the highest altitude reached in the program. Some new and different lessons were learned through the successful flight testing of the HL-10. These lessons, when combined with information from it's sister ship, the M2-F2/F3, provided an excellent starting

Acridones as inducers of HL-60 cell differentiation.

PubMed

Kawaii, S; Tomono, Y; Katase, E; Ogawa, K; Yano, M; Takemura, Y; Ju-ichi, M; Ito, C; Furukawa, H

1999-03-01

Fifteen acridone alkaloids were examined for their activity of induction of human promyelocytic leukemia cell (HL-60) differentiation. HL-60 cells were differentiated into mature monocyte/macrophage by atalaphyllidine (9), atalaphyllinine (12), and des-N-methylnoracronycine (13). The activities of NBT reduction, nonspecific esterase, and phagocytosis, were induced by 2.5 microM of 9, 12, and 13. After a 4-day treatment, 9, 12, and 13 at 10 microM inhibited clonal proliferation of HL-60 cells by 28, 96, and 63%, respectively. The structure-activity relationship established from the results revealed that hydroxyl group at C-1 and prenyl group at C-2 had an important role.

A wide range real-time synchronous demodulation system for the dispersion interferometer on HL-2M

NASA Astrophysics Data System (ADS)

Wu, Tongyu; Zhang, Wei; Yin, Zejie

2017-09-01

A real-time synchronous demodulation system has been developed for the dispersion interferometer on a HL-2M tokamak. The system is based on the phase extraction method which uses a ratio of modulation amplitudes. A high-performance field programmable gate array with pipeline process capabilities is used to realize the real time synchronous demodulation algorithm. A fringe jump correction algorithm is applied to follow the fast density changes of the plasma. By using the Peripheral Component Interconnect Express protocol, the electronics can perform real-time density feedback with a temporal resolution of 100 ns. Some experimental results presented show that the electronics can obtain a wide measurement range of 2.28 × 1022 m-2 with high precision.

Research on the phase adjustment method for dispersion interferometer on HL-2A tokamak

NASA Astrophysics Data System (ADS)

Tongyu, WU; Wei, ZHANG; Haoxi, WANG; Yan, ZHOU; Zejie, YIN

2018-06-01

A synchronous demodulation system is proposed and deployed for CO2 dispersion interferometer on HL-2A, which aims at high plasma density measurements and real-time feedback control. In order to make sure that the demodulator and the interferometer signal are synchronous in phase, a phase adjustment (PA) method has been developed for the demodulation system. The method takes advantages of the field programmable gate array parallel and pipeline process capabilities to carry out high performance and low latency PA. Some experimental results presented show that the PA method is crucial to the synchronous demodulation system and reliable to follow the fast change of the electron density. The system can measure the line-integrated density with a high precision of 2.0 × 1018 m‑2.

HL-10 pilots assist with pilot entry into lifting body

NASA Technical Reports Server (NTRS)

1969-01-01

Not every moment of a test pilot's day is serious business. In a moment of levity, NASA pilots Bill Dana (left) and John A. Manke try to drag Air Force test pilot Peter Hoag away from the HL-10 lifting body while Air Force Major Jerauld R. Gentry helps from the cockpit. These four men were the principal pilots for the HL-10 program. This was not the only prank involving the HL-10 and its pilots. Once 'Captain Midnight' (Gentry) and the 'Midnight skulkers' sneaked into the NASA hangar and put 'U.S. Air Force' on the aircraft using stick-on letters. Later, while Gentry was making a lifting-body flight, his 1954 Ford was 'borrowed' from the parking lot, painted with yellow-green zinc-chromate primer, and decorated with large stick-on flowers about one foot in diameter. After Gentry returned from the flight, he was surprised to see what had happened to his car. The HL-10 was one of five heavyweight lifting-body designs flown at NASA's Flight Research Center (FRC--later Dryden Flight Research Center), Edwards, California, from July 1966 to November 1975 to study and validate the concept of safely maneuvering and landing a low lift-over-drag vehicle designed for reentry from space. Northrop Corporation built the HL-10 and M2-F2, the first two of the fleet of 'heavy' lifting bodies flown by the NASA Flight Research Center. The contract for construction of the HL-10 and the M2-F2 was $1.8 million. 'HL' stands for horizontal landing, and '10' refers to the tenth design studied by engineers at NASA's Langley Research Center, Hampton, Va. After delivery to NASA in January 1966, the HL-10 made its first flight on Dec. 22, 1966, with research pilot Bruce Peterson in the cockpit. Although an XLR-11 rocket engine was installed in the vehicle, the first 11 drop flights from the B-52 launch aircraft were powerless glide flights to assess handling qualities, stability, and control. In the end, the HL-10 was judged to be the best handling of the three original heavy-weight lifting

Synergetic Effects of Runaway and Disruption Induced by VDE on the First Wall Damage in HL-2A

NASA Astrophysics Data System (ADS)

Song, Xianying; Yang, Jinwei; Li, Xu; Yuan, Guoliang; Zhang, Yipo

2012-03-01

The plasma facing component in HL-2A has been damaged seriously after disruption, and for this reason its operation is suspended for maintenance. The experimental phenomena and plasma configurations, calculated by the current filament code (CF-code) using the plasma parameters measured by diagnostics and the signals of the magnetic probes, confirm that the first wall is damaged by the synergetic effects of runaway electrons and disruption induced by a vertical displacement event (VDE). When the plasma column is displaced upward/downward, the strong runaway electrons normally hit the baffle plate of the MP3 or MP1 coil in the upper and lower divertor during the disruption, causing the baffle plates to be holed and wrinkled by the energetic runaway current, and water (for cooling or heating the baffle plates) to leak into the vacuum vessel. Another disastrous consequence is that bellows underlying the baffle plate and outside the coil of MP3 for connecting two segments of the jacket casing pipe are punctured by arcing. The arc may be part of the halo current that forms a complete circuit. The experimental phenomena are indirect but compelling evidence for the existence of a halo current during the disruption and VDE, though the halo current has not been measured by the diagnostics in the HL-2A tokamak.

Development of a real time magnetic island identification system for HL-2A tokamak.

PubMed

Chen, Chao; Sun, Shan; Ji, Xiaoquan; Yin, Zejie

2017-08-01

A novel real time magnetic island identification system for HL-2A is introduced. The identification method is based on the measurement of Mirnov probes and the equilibrium flux constructed by the equilibrium fit (EFIT) code. The system consists of an analog front board and a digital processing board connected by a shield cable. Four octal-channel analog-to-digital convertors are utilized for 100 KHz simultaneous sampling of all the probes, and the applications of PCI extensions for Instrumentation platform and reflective memory allow the system to receive EFIT results simultaneously. A high performance field programmable gate array (FPGA) is used to realize the real time identification algorithm. Based on the parallel and pipeline processing of the FPGA, the magnetic island structure can be identified with a cycle time of 3 ms during experiments.

Development of a real time magnetic island identification system for HL-2A tokamak

NASA Astrophysics Data System (ADS)

Chen, Chao; Sun, Shan; Ji, Xiaoquan; Yin, Zejie

2017-08-01

A novel real time magnetic island identification system for HL-2A is introduced. The identification method is based on the measurement of Mirnov probes and the equilibrium flux constructed by the equilibrium fit (EFIT) code. The system consists of an analog front board and a digital processing board connected by a shield cable. Four octal-channel analog-to-digital convertors are utilized for 100 KHz simultaneous sampling of all the probes, and the applications of PCI extensions for Instrumentation platform and reflective memory allow the system to receive EFIT results simultaneously. A high performance field programmable gate array (FPGA) is used to realize the real time identification algorithm. Based on the parallel and pipeline processing of the FPGA, the magnetic island structure can be identified with a cycle time of 3 ms during experiments.

FGF-23 dysregulates calcium homeostasis and electrophysiological properties in HL-1 atrial cells.

PubMed

Kao, Yu-Hsun; Chen, Yao-Chang; Lin, Yung-Kuo; Shiu, Rong-Jie; Chao, Tze-Fan; Chen, Shih-Ann; Chen, Yi-Jen

2014-08-01

Fibroblast growth factor (FGF)-23 is a key regulator of phosphate homeostasis. Higher FGF-23 levels are correlated with poor outcomes in cardiovascular diseases. FGF-23 can produce cardiac hypertrophy and increase intracellular calcium, which can change cardiac electrical activity. However, it is not clear whether FGF-23 possesses arrhythmogenic potential through calcium dysregulation. Therefore, the purposes of this study were to evaluate the electrophysiological effects of FGF-23 and identify the underlying mechanisms. Patch clamp, confocal microscope with Fluo-4 fluorescence, and Western blot analyses were used to evaluate the electrophysiological characteristics, calcium homeostasis and calcium regulatory proteins in HL-1 atrial myocytes with and without FGF-23 (10 and 25 ng/mL) incubation for 24 h. FGF-23 (25 ng/mL) increased L-type calcium currents, calcium transient and sarcoplasmic reticulum Ca(2+) contents in HL-1 cells. FGF-23 (25 ng/mL)-treated cells (n = 14) had greater incidences (57%, 17% and 15%, P < 0·05) of delayed afterdepolarizations than control (n = 12) and FGF-23 (10 ng/mL)-treated cells (n = 13). Compared with control cells, FGF-23 (25 ng/mL)-treated cells (n = 14) exhibited increased phosphorylation of calcium/calmodulin-dependent protein kinase IIδ and phospholamban (PLB) at threonine 17 but had similar phosphorylation extents of PLB at serine 16, total PLB and sarcoplasmic reticulum Ca(2+) -ATPase protein. Moreover, the FGF receptor inhibitor (PD173074, 10 nM), calmodulin inhibitor (W7, 5 μM) and phospholipase C inhibitor (U73122, 1 μM) attenuated the effects of FGF-23 on calcium/calmodulin-dependent protein kinase II phosphorylation. FGF-23 increases HL-1 cells arrhythmogenesis with calcium dysregulation through modulating calcium-handling proteins. © 2014 Stichting European Society for Clinical Investigation Journal Foundation.

Ascent abort capability for the HL-20

NASA Technical Reports Server (NTRS)

Naftel, J. C.; Talay, T. A.

1993-01-01

The HL-20 has been designed with the capability for rescue of the crew during all phases of powered ascent from on the launch pad until orbital injection. A launch-escape system, consisting of solid rocket motors located on the adapter between the HL-20 and the launch vehicle, provides the thrust that propels the HL-20 to a safe distance from a malfunctioning launch vehicle. After these launch-escape motors have burned out, the adapter is jettisoned and the HL-20 executes one of four abort modes. In three abort modes - return-to-launch-site, transatlantic-abort-landing, and abort-to-orbit - not only is the crew rescued, but the HL-20 is recovered intact. In the ocean-landing-by-parachute abort mode, which occurs in between the return-to-launch-site and the transatlantic-abort-landing modes, the crew is rescued, but the HL-20 would likely sustain damage from the ocean landing. This paper describes the launch-escape system and the four abort modes for an ascent on a Titan III launch vehicle.

The supersonic molecular beam injector as a reliable tool for plasma fueling and physics experiment on HL-2A.

PubMed

Chen, C Y; Yu, D L; Feng, B B; Yao, L H; Song, X M; Zang, L G; Gao, X Y; Yang, Q W; Duan, X R

2016-09-01

On HL-2A tokamak, supersonic molecular beam injection (SMBI) has been developed as a routine refueling method. The key components of the system are an electromagnetic valve and a conic nozzle. The valve and conic nozzle are assembled to compose the simplified Laval nozzle for generating the pulsed beam. The appurtenance of the system includes the cooling system serving the cooled SMBI generation and the in situ calibration component for quantitative injection. Compared with the conventional gas puffing, the SMBI features prompt response and larger fueling flux. These merits devote the SMBI a good fueling method, an excellent plasma density feedback control tool, and an edge localized mode mitigation resource.

Raman spectrum reveals Mesenchymal stem cells inhibiting HL60 cells growth

NASA Astrophysics Data System (ADS)

Su, Xin; Fang, Shaoyin; Zhang, Daosen; Zhang, Qinnan; Lu, Xiaoxu; Tian, Jindong; Fan, Jinping; Zhong, Liyun

2017-04-01

Though some research results reveals that Mesenchymal stem cells (MSCs) have the ability of inhibiting tumor cells proliferation, it remains controversial about the precise interaction mechanism during MSCs and tumor cells co-culture. In this study, combing Raman spectroscopic data and principle component analysis (PCA), the biochemical changes of MSCs or Human promyelocytic leukemia (HL60) cells during their co-culture were presented. The obtained results showed that some main Raman peaks of HL60 assigned to nucleic acids or proteins were greatly higher in intensity in the late stage of co-culture than those in the early stage of co-culture while they were still lower relative to the control group, implicating that the effect of MSCs inhibiting HL60 proliferation appeared in the early stage but gradually lost the inhibiting ability in the late stage of co-culture. Moreover, some other peaks of HL60 assigned to proteins were decreased in intensity in the early stage of co-culture relative to the control group but rebounded to the level similar to the control group in the late stage, showing that the content and structure changes of these proteins might be generated in the early stage but returned to the original state in the late stage of co-culture. As a result, in the early stage of MSCs-HL60 co-culture, along with the level of Akt phosphorylation of HL60 was lowered relative to its control group, the proliferation rate of HL60 cells was decreased. And in the late stage of co-culture, along with the level of Akt phosphorylation was rebounded, the reverse transfer of Raman peaks within 875-880 cm- 1 appeared, thus MSCs lost the ability to inhibit HL60 growth and HL60 proliferation was increased. In addition, it was observed that the peak at 811 cm- 1, which is a marker of RNA, was higher in intensity in the late stage than that in the control group, indicating that MSCs might be differentiated into myofibroblast-like MSCs. In addition, PCA results also exhibited

HMG CoA Lyase (HL): Mutation detection and development of a bacterial expression system for screening the activity of mutant alleles from HL-deficient patients

DOE Office of Scientific and Technical Information (OSTI.GOV)

Robert, M.F.; Ashmarina, L.; Poitier, E.

1994-09-01

HL catalyzes the last step of ketogenesis, and autosomal recessive HL deficiency in humans can cause episodes of hypoglycemia and coma. Structurally, HL is a dimer of identical 325-residue peptides which requires a reducing environment to maintain activity. We cloned the human and mouse HL cDNAs and genes and have performed mutation analysis on cells from 30 HL-deficient probands. Using SSCP and also genomic Southern analysis we have identified putative mutations on 53/60 alleles of these patients (88%). To date, we have found 20 mutations: 3 large deletions, 4 termination mutations, 5 frameshift mutations, and 8 missense mutations which wemore » suspect to be pathogenic based on evolutionary conservation and/or our previous studies on purified HL protein. We have also identified 3 polymorphic variants. In order to directly test the activity of the missense mutations, we established a pGEX-based system, using a glutathione S transferase (GST)-HL fusion protein. Expressed wild-type GST-HL was insoluble. We previously located a reactive Cys at the C-terminus of chicken HL which is conserved in human HL. We produced a mutant HL peptide, C323S, which replaced Cys323 with Ser. Purified C323S is soluble and has similar kinetics to wild-type HL. C323S-containing GST-HL is soluble and enzymatically active. We are cloning and expressing the 8 missense mutations.« less

SKP2 siRNA inhibits the degradation of P27kip1 and down-regulates the expression of MRP in HL-60/A cells.

PubMed

Xiao, Jie; Yin, Songmei; Li, Yiqing; Xie, Shuangfeng; Nie, Danian; Ma, Liping; Wang, Xiuju; Wu, Yudan; Feng, Jianhong

2009-08-01

S-phase kinase-associated protein 2 (SKP2) gene is a tumor suppressor gene, and is involved in the ubiquitin-mediated degradation of P27kip1. SKP2 and P27kip1 affect the proceeding and prognosis of leukemia through regulating the proliferation, apoptosis and differentiation of leukemia cells. In this study, we explored the mechanism of reversing of HL-60/A drug resistance through SKP2 down-regulation. HL-60/A cells were nucleofected by Amaxa Nucleofector System with SKP2 siRNA. The gene and protein expression levels of Skp2, P27kip1, and multi-drug resistance associated protein (MRP) were determined by reverse transcription-polymerase chain reaction and western blot analysis, respectively. The cell cycle was analyzed by flow cytometry. The 50% inhibitory concentration value was calculated using cytotoxic analysis according to the death rate of these two kinds of cells under different concentrations of chemotherapeutics to compare the sensitivity of the cells. HL-60/A cells showed multi-drug resistance phenotype characteristic by cross-resistance to adriamycin, daunorubicin, and arabinosylcytosine, due to the expression of MRP. We found that the expression of SKP2 was higher in HL-60/A cells than in HL-60 cells, but the expression of P27kip1 was lower. The expression of SKP2 in HL-60/A cells nucleofected by SKP2 siRNA was down-regulated whereas the protein level of P27kip1 was up-regulated. Compared with the MRP expression level in the control group (nucleofected by control siRNA), the mRNA and protein expression levels of MRP in HL-60/A cells nucleofected by SKP2 siRNA were lower, and the latter cells were more sensitive to adriamycin, daunorubicin, and arabinosylcytosine. Down-regulating the SKP2 expression and arresting cells in the G0/G1 phase improve drug sensitivity of leukemia cells with down-regulated MRP expression.

Mangiferin induces cell cycle arrest at G2/M phase through ATR-Chk1 pathway in HL-60 leukemia cells.

PubMed

Peng, Z G; Yao, Y B; Yang, J; Tang, Y L; Huang, X

2015-05-12

This study aimed to determine the effect of mangiferin on the cell cycle in HL-60 leukemia cells and expression of the cell cycle-regulatory genes Wee1, Chk1 and CDC25C and to further investigate the molecular mechanisms of the antileukemic action of mangiferin. The inhibitory effect of mangiferin on HL-60 leukemia cell proliferation was determined by the MTT assay. The impact of mangiferin on the HL-60 cell cycle was evaluated by flow cytometry. After the cells were treated with different concentrations of mangiferin, the expression levels of Wee1, Chk1 and CDC25C mRNA were determined by RT-PCR, and Western blot was used to evaluate the expression levels of cdc25c, cyclin B1, and Akt proteins. The inhibition of HL-60 cell growth by mangiferin was dose- and time-dependent. After treatment for 24 h, cells in G2/M phase increased, and G2/M phase arrest appeared with increased mRNA expression of Wee1, Chk1 and CDC25C. Mangiferin inhibited Chk1 and cdc25c mRNA expression at high concentrations and induced Wee1 mRNA expression in a dose-dependent manner. It significantly inhibited ATR, Chk1, Wee1, Akt, and ERK1/2 phosphorylation but increased cdc2 and cyclin B1 phosphorylation. Furthermore, mangiferin reduced cdc25c, cyclin B1, and Akt protein levels while inducing Wee1 protein expression. It also antagonized the phosphorylation effect of vanadate on ATR, and the phosphorylation effect of EGF on Wee1. These findings indicated that mangiferin inhibits cell cycle progression through the ATR-Chk1 stress response DNA damage pathway, leading to cell cycle arrest at G2/M phase in leukemia cells.

Induction of differentiation in human promyelocytic HL-60 leukemia cells activates p21, WAF1/CIP1, expression in the absence of p53.

DOE Office of Scientific and Technical Information (OSTI.GOV)

Jiang, H.; Lin, J.; Su, Z.-Z.

The melanoma differentiation associated gene, mda-6, which is identical to the P53-inducible gene WAF1/CIP1, encodes an M(r) 21,000 protein (p21) that can directly inhibit cell growth by repressing cyclin dependent kinases. mda-6 was identified using subtraction hybridization by virtue of its enhanced expression in human melanoma cells induced to terminally differentiate by treatment with human fibroblast interferon and the anti-leukemic compound mezerein (Jiang and Fisher, 1993). In the present study, we demonstrate that mda-6 (WAF1/CIP1) is an immediate early response gene induced during differentiation of the promyelocytic HL-60 leukemia cell line along the granulocytic or macrophage/monocyte pathway. mda-6 gene expressionmore » in HL-60 cells is induced within 1 to 3 h during differentiation along the macrophage/monocyte pathway evoked by 12-0-tetradecanoyl phorbol-13-acetate (TPA) or 1,25-dihydroxyvitamin D3 (Vit D3) or the granulocytic pathway produced by retinoic acid (RA) or dimethylsulfoxide (DMSO). Immunoprecipitation analyses using an anti-p21 antibody indicate a temporal induction of p21 protein following treatment with TPA, DMSO or RA. A relationship between rapid induction of mda-6 gene expression and differentiation is indicated by a delay in this expression in an HL-60 cell variant resistant to TPA-induced growth arrest and differentiation. A similar delay in mda-6 gene expression is not observed in Vit D3 treated TPA-resistant variant cells that are also sensitive to induction of monocytic differentiation. Since HL-60 cells have a null-p53 phenotype, these results demonstrate that p21 induction occurs during initiation of terminal differentiation in a p53-independent manner. In this context, p21 may play a more global role in growth control and differentiation than originally envisioned.« less

Activation of protein kinase C induces nuclear translocation of RFX1 and down-regulates c-myc via an intron 1 X box in undifferentiated leukemia HL-60 cells.

PubMed

Chen, L; Smith, L; Johnson, M R; Wang, K; Diasio, R B; Smith, J B

2000-10-13

Treatment of human promyelocytic leukemia cells (HL-60) with phorbol 12-myristate 13-acetate (PMA) is known to decrease c-myc mRNA by blocking transcription elongation at sites near the first exon/intron border. Treatment of HL-60 cells with either PMA or bryostatin 1, which acutely activates protein kinase C (PKC), decreased the levels of myc mRNA and Myc protein. The inhibition of Myc synthesis accounted for the drop in Myc protein, because PMA treatment had no effect on Myc turnover. Treatment with PMA or bryostatin 1 increased nuclear protein binding to MIE1, a c-myc intron 1 element that defines an RFX1-binding X box. RFX1 antiserum supershifted MIE1-protein complexes. Increased MIE1 binding was independent of protein synthesis and abolished by a selective PKC inhibitor, which also prevented the effect of PMA on myc mRNA and protein levels and Myc synthesis. PMA treatment increased RFX1 in the nuclear fraction and decreased it in the cytosol without affecting total RFX1. Transfection of HL-60 cells with myc reporter gene constructs showed that the RFX1-binding X box was required for the down-regulation of reporter gene expression by PMA. These findings suggest that nuclear translocation and binding of RFX1 to the X box cause the down-regulation of myc expression, which follows acute PKC activation in undifferentiated HL-60 cells.

Integrating Genomic Resources with Electronic Health Records using the HL7 Infobutton Standard

PubMed Central

Overby, Casey Lynnette; Del Fiol, Guilherme; Rubinstein, Wendy S.; Maglott, Donna R.; Nelson, Tristan H.; Milosavljevic, Aleksandar; Martin, Christa L.; Goehringer, Scott R.; Freimuth, Robert R.; Williams, Marc S.

2016-01-01

Summary Background The Clinical Genome Resource (ClinGen) Electronic Health Record (EHR) Workgroup aims to integrate ClinGen resources with EHRs. A promising option to enable this integration is through the Health Level Seven (HL7) Infobutton Standard. EHR systems that are certified according to the US Meaningful Use program provide HL7-compliant infobutton capabilities, which can be leveraged to support clinical decision-making in genomics. Objectives To integrate genomic knowledge resources using the HL7 infobutton standard. Two tactics to achieve this objective were: (1) creating an HL7-compliant search interface for ClinGen, and (2) proposing guidance for genomic resources on achieving HL7 Infobutton standard accessibility and compliance. Methods We built a search interface utilizing OpenInfobutton, an open source reference implementation of the HL7 Infobutton standard. ClinGen resources were assessed for readiness towards HL7 compliance. Finally, based upon our experiences we provide recommendations for publishers seeking to achieve HL7 compliance. Results Eight genomic resources and two sub-resources were integrated with the ClinGen search engine via OpenInfobutton and the HL7 infobutton standard. Resources we assessed have varying levels of readiness towards HL7-compliance. Furthermore, we found that adoption of standard terminologies used by EHR systems is the main gap to achieve compliance. Conclusion Genomic resources can be integrated with EHR systems via the HL7 Infobutton standard using OpenInfobutton. Full compliance of genomic resources with the Infobutton standard would further enhance interoperability with EHR systems. PMID:27579472

Overview of recent HL-2A experiments

NASA Astrophysics Data System (ADS)

Duan, X. R.; Liu, Yi; Xu, M.; Yan, L. W.; Xu, Y.; Song, X. M.; Dong, J. Q.; Ding, X. T.; Chen, L. Y.; Lu, B.; Liu, D. Q.; Rao, J.; Xuan, W. M.; Yang, Q. W.; Zheng, G. Y.; Zou, X. L.; Liu, Y. Q.; Zhong, W. L.; Zhao, K. J.; Ji, X. Q.; Mao, W. C.; Wang, Q. M.; Li, Q.; Cao, J. Y.; Cao, Z.; Lei, G. J.; Zhang, J. H.; Li, X. D.; Bai, X. Y.; Cheng, J.; Chen, W.; Cui, Z. Y.; Delpech, L.; Diamond, P. H.; Dong, Y. B.; Ekedahl, A.; Hoang, T.; Huang, Y.; Ida, K.; Itoh, K.; Itoh, S.-I.; Isobe, M.; Inagaki, S.; Mazon, D.; Morita, S.; Peysson, Y.; Shi, Z. B.; Wang, X. G.; Xiao, G. L.; Yu, D. L.; Yu, L. M.; Zhang, Y. P.; Zhou, Y.; Cui, C. H.; Feng, B. B.; Huang, M.; Li, Y. G.; Li, B.; Li, G. S.; Li, H. J.; Li, Qing; Peng, J. F.; Wang, Y. Q.; Yuan, B. S.; Liu, Yong; HL-2A Team

2017-10-01

Since the last Fusion Energy Conference, significant progress has been made in the following areas. The first high coupling efficiency low-hybrid current drive (LHCD) with a passive-active multi-junction (PAM) antenna was successfully demonstrated in the H-mode on the HL-2A tokamak. Double critical impurity gradients of electromagnetic turbulence were observed in H-mode plasmas. Various ELM mitigation techniques have been investigated, including supersonic molecular beam injection (SMBI), impurity seeding, resonant magnetic perturbation (RMP) and low-hybrid wave (LHW). The ion internal transport barrier was observed in neutral beam injection (NBI) heated plasmas. Neoclassical tearing modes (NTMs) driven by the transient perturbation of local electron temperature during non-local thermal transport events have been observed, and a new type of non-local transport triggered by the ion fishbone was found. A long-lasting runaway electron plateau was achieved after argon injection and the runaway current was successfully suppressed by SMBI. It was found that low-n Alfvénic ion temperature gradient (AITG) modes can be destabilized in ohmic plasmas, even with weak magnetic shear and low-pressure gradients. For the first time, the synchronization of geodesic acoustic mode (GAM) and magnetic fluctuations was observed in edge plasmas, revealing frequency entrainment and phase lock. The spatiotemporal features of zonal flows were also studied using multi-channel correlation Doppler reflectometers.

[Effect of Magnolol on Proliferation and Apoptosis of HL-60 Cells and Its Molecular Mechanism].

PubMed

Fang, Ke; Yuan, Xiao-Fen; Liao, Qiong; Zhang, Zhi-Yong; Song, Guan-Hua; Guo, Qiang; Ren, Xia; Jiang, Guo-Sheng

2016-04-01

To investigate the effect of magnolol on proliferation and apoptosis of HL-60 cells and its mechanism. MTT assay was used to measure the proliferation of HL-60 cells after treatment with different concentration of magnolol (5, 10, 20, 40, 80 and 160 µg/ml). The morphological changes of HL-60 cells were examined by light microscopy, and DAPI staining was performed to observe the nuclear morphology of HL-60 cells. The early cell apoptosis was detected by flow cytometry with Annexin V-FITC/PI double-staining. RT-PCR was carried out to examine the mRNA expression of BAX and BCL-2. Western blot was performed to detect the protein expression of caspase family. The magnolol inhibited HL-60 cell proliferation, and the inhibitory rate of cell proliferation increased significantly in a dose- and time- dependent manner (P < 0.05). HL-60 cells became small, even apoptotic bodies appeared after treatment with magnolol. In addition, nuclear condensation or fragmentation could be observed, which is the typical morphological features of apoptosis. When HL-60 cells were treated with 40 µg/ml of magnolol for 24 h, the ratio of early apoptotic cells reached to (11.7 ± 2.4) %, which was significant different from control (1.4 ± 1.1) % (P < 0.05). RT-PCR results showed that treatment of HL-60 cells with magnolol up-regulated the expression of BAX, whereas down-regulated the expression of BCL-2. Western blot results showed that the cleavages of caspase-3, -8 and -9 were significantly enhanced by magnolol. The magnolol can significantly inhibit the proliferation of HL-60 cells and induce the apoptosis of HL-60 cells, which may occur through up-regulation of BAX, down-regulation of BCL-2 and the activation of caspases.

RF assisted Glow Discharge Condition experiment for SST-1 Tokamak

NASA Astrophysics Data System (ADS)

Raval, Dilip; Khan, Ziauddin; George, Siju; Dhanani, Kalpeshkumar R.; Paravastu, Yuvakiran; Semwal, Pratibha; Thankey, Prashant; Shoaib Khan, Mohammad; Kakati, Bharat; Pradhan, Subrata

2017-04-01

Impurity control reduces the radiation loss from plasma and hence enhances the plasma operation. Oxygen and water vapors are the most common impurities in tokamak devices. Water vapour can be reduced with extensive baking while in order to have a significant reduction in oxygen it is necessary to use glow discharge condition (GDC). RF assisted glow discharge cleaning system will be implemented to remove low z impurities at PFC installed SST-1 vacuum vessel. A RF assisted Glow discharge conditioning is studied at laboratory to find the optimum operating parameters in a view to implement at SST-1 tokamak. Helium is used as a fuel gas in the present experiment. It is observed that the ultimate impurity level is reduced significantly below to the accepted level for plasma operation after RF assisted GDC. The experimental findings of RF assisted Glow discharge conditioning is discussed in details in this paper.

The Effect of TNF-α Blocker HL036337 and Its Best Concentration to Inhibit Dry Eye Inflammation.

PubMed

Choi, Wungrak; Noh, Hyemi; Yeo, Areum; Jang, Hanmil; Ahn, Hyea Kyung; Song, Yeon Jung; Lee, Hyung Keun

2016-08-01

Dry eye syndrome is commonly thought of as an inflammatory disease, and we have previously presented data showing the effectiveness of topical TNF-α blocker agents for the treatment of this condition. The purpose of this study was to investigate the effectiveness of the TNF-α blocking agent HL036337 compared to cyclosporine A for the treatment of dry eye induced inflammation in order to establish whether HL036337 represents a more effective method for suppressing inflammation. The efficacy of HL036337 and cyclosporine A was determined using an experimental murine dry eye model. The TNF-α blocker HL036337 is a modified form of TNF receptor I. Using dry eye induced C57BL/6 mice (n = 45), corneal erosion was measured at day 4 and 7 after topical treatment with cyclosporine A or HL036337. To determine the effective treatment dose, 0.25, 0.5, 1, 2.5, and 5 mg/mL of HL036337 were topically administered twice per day to dry eye induced murine corneas for 1 week. The optimal concentration of the TNF-α blocker HL036337 for treatment of dry eye induced corneal erosion was determined to be 1 mg/mL. Dry eye induced corneal erosion was improved after 1 week with topically applied cyclosporine A and HL036337 at 1 mg/mL. HL036337 administered topically at 1 mg/mL effectively improved corneal erosion induced by dry eye. This finding may also suggest that inhibition of TNF-α can improve dry eye syndrome.

HMG-CoA lyase (HL) gene: Cloning and characterization of the 5{prime} end of the mouse gene, gene targeting in ES cells, and demonstration of large deletions in three HL-deficient patients

DOE Office of Scientific and Technical Information (OSTI.GOV)

Wang, S.; Robert, M.F.; Mitchell, G.A.

1994-09-01

3-hydroxy-3-methylglutaryl CoA lyase (HL) is a mitochondrial matrix enzyme which catalyzes the last step of leucine catabolism and of ketogenesis. Autosomal recessive HL deficiency in humans results in episodes of hypoglycemia and coma. We are interested in the pathophysiology of HL deficiency as a model for both amino acid and fatty acid inborn errors. We have cloned the human and mouse HL genes. In order to analyze the 5{prime} nontranslated region of mouse HL gene, we cloned and sequenced a 1.8 kb fragment containing the 5{prime} extremity including exon 1 and about 1.6 kb of 5{prime} nontranslated sequence. The regionmore » surrounding exon 1 is CpG-rich (66.4%). Using the criteria of West, the Observed/Expected ratio for CpG dinucleotides is 0.7 ({ge}0.6 is consistent with a CpG island). We are carrying out primer extension and RNase protection experiments to determine the transcription initiation site. We constructed a gene targeting vector by introducing the neomycin resistance gene into exon 2 of a 7.5 kb genomic subclone of the mouse HL gene. Targeting was performed by electroporating 10 mg linearized vector into 10{sup 7} ES cells and selecting for 12 days with G418. 5/228 colonies (2.2%) had homologous recombination as shown by PCR screening and Southern analysis. We are microinjecting the 5 targeted clones into blastocysts to create an HL-deficient mouse. To date we have obtained two chimeras with contributions of 95% and 55% from 129, by coat color estimates. Three of 27 (11%) of the HL-deficient patients studied were suggested by genomic Southern analysis to be homozygous for large intragenic deletions. We confirmed this and defined the boundaries using exonic PCR.« less

The inhibitory activity of HL-7 and HL-10 peptide from scorpion venom (Hemiscorpius lepturus) on angiotensin converting enzyme: Kinetic and docking study.

PubMed

Setayesh-Mehr, Zahra; Asoodeh, Ahmad

2017-12-01

The hypertension is one of the highest risk factors for stroke, myocardial infarction, vascular disease and chronic kidney disease. Angiotensin converting enzyme (ACE) has an important role in the physiological regulation of cardiovascular system. ACE inhibition is a key purpose for hypertension treatment. In this study, two peptides named HL-7 with the sequence of YLYELAR (MW: 927.07Da) and HL-10 with the sequence of AFPYYGHHLG (MW: 1161.28Da) were identified from scorpion venom of H. lepturus. The inhibitory activity of HL-7 and HL-10 was examined on rabbit ACE. The inhibition mechanisms were assayed by kinetic and docking studies. The IC 50 values for ACE inhibition of HL-7 and HL-10 were 9.37µM and 17.22µM, respectively. Lineweaver-Burk plots showed that two peptides inhibited rabbit ACE with competitive manner. The molecular docking conformed experimental results and showed that the two peptides interacted with N-domain and C-domain active sites. Also, docking study revealed that the two peptides can form hydrogen and hydrophobic bonds at their binding sites. Both peptides had higher affinity to N-domain. Our results showed that HL-7 exhibited more strong interactions with amino acids at active site. It seems that HL-10 peptide could occupy more space, thereby inhibiting the substrate entrance to active site. Copyright © 2017 Elsevier Inc. All rights reserved.

Isolation of furocoumarins from bergamot fruits as HL-60 differentiation-inducing compounds.

PubMed

Kawaii, S; Tomono, Y; Katase, E; Ogawa, K; Yano, M

1999-10-01

The HL-60 differentiation-inducing compounds in bergamot fruits were isolated with column chromatography and identified as bergamottin, bergapten, and citropten by (1)H and (13)C NMR. Their HL-60 differentiation-inducing activity was measured by examining nitro blue tetrazolium (NBT) reducing, nonspecific acid esterase (NSE), specific esterase (SE), and phagocytic activities, and bergamottin showed the strongest activity among the coumarins isolated from bergamot fruits. The structure-activity relationship obtained from HL-60 differentiation assay suggests that hydrophobicity of furocoumarins is correlated with their activity.

Gas Fuelling System for SST-1Tokamak

NASA Astrophysics Data System (ADS)

Dhanani, Kalpesh; Raval, D. C.; Khan, Ziauddin; Semwal, Pratibha; George, Siju; Paravastu, Yuvakiran; Thankey, Prashant; Khan, M. S.; Pradhan, Subrata

2017-04-01

SST-1 Tokamak, the first Indian Steady-state Superconducting experimental device is at present under operation in the Institute for Plasma Research. For plasma break down & initiation, piezoelectric valve based gas feed system is implemented as a primary requirement due to its precise control, easy handling, low construction and maintenance cost and its flexibility in the selection of the working gas. Hydrogen gas feeding with piezoelectric valve is used in the SST-1 plasma experiments. The piezoelectric valves used in SST-1 are remotely driven by a PXI based platform and are calibrated before each SST-1 plasma operation with precise control. This paper will present the technical development and the results of the gas fuelling system of SST-1.

Recent Progress of the HL-2A Multi-Channel HCOOH Laser Interferometer/Polarimeter

NASA Astrophysics Data System (ADS)

Li, Yonggao; Zhou, Yan; Deng, Zhongchao; Li, Yuan; Yi, Jiang; Wang, Haoxi

2015-05-01

A multichannel methanoic acid (HCOOH, λ = 432.5 μm) laser interferometer/polarimeter is being developed from the previous eight-channel hydrogen cyanide (HCN, λ = 337 μm) laser interferometer in the HL-2A tokamak. A conventional Michelson-type interometer is used for the electron density measurement, and a Dodel-Kunz-type polarimeter is used for the Faraday rotation effect measurement, respectively. Each HCOOH laser can produce a linearly polarized radiation at a power lever of ˜30 mW, and a power stability <10% in 50 min. A beam waist (diameter d0 ≈12.0 mm, about 200 mm away from the outlet) is finally determined through a chopping modulation technique. The latest optical layout of the interferometer/polarimeter has been finished, and the hardware data processing system based on the fast Fourier transform phase-comparator technique is being explored. In order to demonstrate the feasibility of the diagnostic scheme, two associated bench simulation experiments were carried out in the laboratory, in which the plasma was simulated by a piece of polytetrafluoroethene plate, and the Faraday rotation effect was simulated by a rotating half-wave plate. Simulation results agreed well with the initial experimental conditions. At present, the HCOOH laser interferometer/polarimeter system is being assembled on HL-2A, and is planned to be applied in the 2014-2015 experimental campaign. supported by the National Magnetic Confinement Fusion Science Programs of China (Nos. 2010GB101002 and 2014GB109001), and National Natural Science Foundation of China (Nos. 11075048 and 11275059)

1D and 2D assembly structures by imidazole···chloride hydrogen bonds of iron(II) complexes [Fe(II)(HL(n-Pr))3]Cl·Y (HL(n-Pr) = 2-methylimidazol-4-yl-methylideneamino-n-propyl; Y = AsF6, BF4) and their spin states.

PubMed

Fujinami, Takeshi; Nishi, Koshiro; Matsumoto, Naohide; Iijima, Seiichiro; Halcrow, Malcolm A; Sunatsuki, Yukinari; Kojima, Masaaki

2011-12-07

Two Fe(II) complexes fac-[Fe(II)(HL(n-Pr))(3)]Cl·Y (Y = AsF(6) (1) and BF(4) (2)) were synthesized, where HL(n-Pr) is 2-methylimidazole-4-yl-methylideneamino-n-propyl. Each complex-cation has the same octahedral N(6) geometry coordinated by three bidentate ligands and assumes facial-isomerism, fac-[Fe(II)(HL(n-Pr))(3)](2+) with Δ- and Λ-enantiomorphs. Three imidazole groups per Δ- or Λ-fac-[Fe(II)(HL(n-Pr))(3)](2+) are hydrogen-bonded to three Cl(-) ions or, from the viewpoint of the Cl(-) ion, one Cl(-) ion is hydrogen-bonded to three neighbouring fac-[Fe(II)(HL(n-Pr))(3)](2+) cations. The 3 : 3 NH···Cl(-) hydrogen bonds between Δ- or Λ-fac-[Fe(II)(HL(n-Pr))(3)](2+) and Cl(-) generate two kinds of assembly structures. The directions of the 3 : 3 NH···Cl(-) hydrogen bonds and hence the resulting assembly structures are determined by the size of the anion Y, though Y is not involved into the network structure and just accommodated in the cavity. Compound 1 has a 1D ladder structure giving a larger cavity, in which the Δ- and Λ-fac-[Fe(II)(HL(n-Pr))(3)](2+) enantiomorphs are bridged by two NH···Cl(-) hydrogen bonds. Compound 2 has a 2D network structure with a net unit of a cyclic trimer of {fac-[Fe(II)(HL(n-Pr))(3)](2+)···Cl(-)}(3) giving a smaller cavity, in which Δ- or Λ-fac-[Fe(II)(HL(n-Pr))(3)](2+) species with the same chirality are linked by NH···Cl(-) hydrogen bonds to give a homochiral 2D network structure. Magnetic susceptibility and Mössbauer spectral measurements demonstrated that compound 1 showed an abrupt one-step spin crossover with 4.0 K thermal hysteresis of T(c↓) = 125.5 K and T(c↑) = 129.5 K and compound 2 showed no spin transition and stayed in the high-spin state over the 5-300 K temperature range.

L1 track triggers for ATLAS in the HL-LHC

DOE PAGES

Lipeles, E.

2012-01-01

The HL-LHC, the planned high luminosity upgrade for the LHC, will increase the collision rate in the ATLAS detector approximately a factor of 5 beyond the luminosity for which the detectors were designed, while also increasing the number of pile-up collisions in each event by a similar factor. This means that the level-1 trigger must achieve a higher rejection factor in a more difficult environment. This presentation discusses the challenges that arise in this environment and strategies being considered by ATLAS to include information from the tracking systems in the level-1 decision. The main challenges involve reducing the data volumemore » exported from the tracking system for which two options are under consideration: a region of interest based system and an intelligent sensor method which filters on hits likely to come from higher transverse momentum tracks.« less

Bridging the Gap between HL7 CDA and HL7 FHIR: A JSON Based Mapping.

PubMed

Rinner, Christoph; Duftschmid, Georg

2016-01-01

The Austrian electronic health record (EHR) system ELGA went live in December 2016. It is a document oriented EHR system and is based on the HL7 Clinical Document Architecture (CDA). The HL7 Fast Healthcare Interoperability Resources (FHIR) is a relatively new standard that combines the advantages of HL7 messages and CDA Documents. In order to offer easier access to information stored in ELGA we present a method based on adapted FHIR resources to map CDA documents to FHIR resources. A proof-of-concept tool using Java, the open-source FHIR framework HAPI-FHIR and publicly available FHIR servers was created to evaluate the presented mapping. In contrast to other approaches the close resemblance of the mapping file to the FHIR specification allows existing FHIR infrastructure to be reused. In order to reduce information overload and facilitate the access to CDA documents, FHIR could offer a standardized way to query CDA data on a fine granular base in Austria.

Implementing a DICOM-HL7 interface application

NASA Astrophysics Data System (ADS)

Fritz, Steven L.; Munjal, Sunita; Connors, James; Csipo, Deszu

1995-05-01

The DICOM standard, in addition to resolving certain problems with the ACR-NEMA 2.0 standard regarding network support and the clinical data dictionary, added new capabilities, in the form of study content notification and patient, study and results management services, intended to assist in interfacing between PACS and HIS or RIS systems. We have defined and implemented a mechanism that allows a DICOM application entity (AE) to interrogate an HL7 based RIS using DICOM services. The implementation involved development of a DICOM- HL7 gateway which converted between DICOM and HL7 messages to achieve the desired retrieval capability. This mechanism, based on the DICOM query/retrieve service, was used to interface a DeJarnette Research film digitizer to an IDXrad RIS at the University of Maryland Medical Systems hospital in Baltimore, Maryland. A C++ class library was developed for both DICOM and HL7 massaging, with several constructors used to convert between the two standards.

HL-10 mounted on a pedestal in front of the Dryden main gate at sunset

NASA Technical Reports Server (NTRS)

1992-01-01

The HL-10 Lifting Body, as shown here, is currently displayed on a pedestal in front of the main gate at NASA's Dryden Flight Research Center, Edwards, California. The HL-10 was one of five heavyweight lifting-body designs flown at NASA's Flight Research Center (FRC--later Dryden Flight Research Center), Edwards, California, from July 1966 to November 1975 to study and validate the concept of safely maneuvering and landing a low lift-over-drag vehicle designed for reentry from space. Northrop Corporation built the HL-10 and M2-F2, the first two of the fleet of 'heavy' lifting bodies flown by the NASA Flight Research Center. The contract for construction of the HL-10 and the M2-F2 was $1.8 million. 'HL' stands for horizontal landing, and '10' refers to the tenth design studied by engineers at NASA's Langley Research Center, Hampton, Va. After delivery to NASA in January 1966, the HL-10 made its first flight on Dec. 22, 1966, with research pilot Bruce Peterson in the cockpit. Although an XLR-11 rocket engine was installed in the vehicle, the first 11 drop flights from the B-52 launch aircraft were powerless glide flights to assess handling qualities, stability, and control. In the end, the HL-10 was judged to be the best handling of the three original heavy-weight lifting bodies (M2-F2/F3, HL-10, X-24A). The HL-10 was flown 37 times during the lifting body research program and logged the highest altitude and fastest speed in the Lifting Body program. On Feb. 18, 1970, Air Force test pilot Peter Hoag piloted the HL-10 to Mach 1.86 (1,228 mph). Nine days later, NASA pilot Bill Dana flew the vehicle to 90,030 feet, which became the highest altitude reached in the program. Some new and different lessons were learned through the successful flight testing of the HL-10. These lessons, when combined with information from it's sister ship, the M2-F2/F3, provided an excellent starting point for designers of future entry vehicles, including the Space Shuttle.

Security architecture for HL/7 message interchange.

PubMed

Chen, T S; Liao, B S; Lin, M G; Gough, T G

2001-01-01

The promotion of quality medical treatment is very important to the healthcare providers as well as to patients. It requires that the medical resources of different hospitals be combined to ensure that medical information is shared and that resources are not wasted. A computer-based patient record is one of the best methods to accomplish the interchange of the patient's clinical data. In our system, the Health Level/Seven (HL/7) format is used for the interchange of the clinical data, as it has been supported by many healthcare providers and become a â standard'. The security of the interchange of clinical data is a serious issue for people using the Internet for data communication. Several international well-developed security algorithms, models and secure policies are adopted in the design of a security handler for an HL/7 architecture. The goal of our system is to combine our security system with the end-to-end communication systems constructed from the HL/7 format to establish a safe delivery channel. A suitable security interchange environment is implemented to address some shortcomings in clinical data interchange. located at the application layer of the ISO/OSI reference model. The medical message components, sub-components, and related types of message event are the primary goals of the HL/7 protocols. The patient management system, the doctor's system for recording his advice, examination and diagnosis as well as any financial management system are all covered by the HL/7 protocols. Healthcare providers and hospitals in Taiwan are very interested in developing the HL/7 protocols as a common standard for clinical data interchange.

Working with the HL7 metamodel in a Model Driven Engineering context.

PubMed

Martínez-García, A; García-García, J A; Escalona, M J; Parra-Calderón, C L

2015-10-01

HL7 (Health Level 7) International is an organization that defines health information standards. Most HL7 domain information models have been designed according to a proprietary graphic language whose domain models are based on the HL7 metamodel. Many researchers have considered using HL7 in the MDE (Model-Driven Engineering) context. A limitation has been identified: all MDE tools support UML (Unified Modeling Language), which is a standard model language, but most do not support the HL7 proprietary model language. We want to support software engineers without HL7 experience, thus real-world problems would be modeled by them by defining system requirements in UML that are compliant with HL7 domain models transparently. The objective of the present research is to connect HL7 with software analysis using a generic model-based approach. This paper introduces a first approach to an HL7 MDE solution that considers the MIF (Model Interchange Format) metamodel proposed by HL7 by making use of a plug-in developed in the EA (Enterprise Architect) tool. Copyright © 2015 Elsevier Inc. All rights reserved.

OHD/HL - Staff

Science.gov Websites

Laboratory Branches Hydrologic Software Engineering Branch (HSEB) Hydrologic Science and Modeling Branch (HSMB) General Info Publications Documentation Software Standard and Guidelines Contact Us HL Staff resources and services. Staff Directory Chief, Hydrology Laboratory; Chief, Hydrologic Software Engineering

NLS-RARα promotes proliferation and inhibits differentiation in HL-60 cells.

PubMed

Hu, Xiu-Xiu; Zhong, Liang; Zhang, Xi; Gao, Yuan-Mei; Liu, Bei-Zhong

2014-01-01

A unique mRNA produced in leukemic cells from a t(15;17) acute promyelocytic leukemia (APL) patient encodes a fusion protein between the retinoic acid receptor α (RARα) and a myeloid gene product called PML. Studies have reported that neutrophil elastase (NE) cleaves bcr-1-derived PML-RARα in early myeloid cells, leaving only the nuclear localization signal (NLS) of PML attached to RARα. The resultant NLS-RARα fusion protein mainly localizes to, and functions within, the cell nucleus. It is speculated that NLS-RARα may act in different ways from the wild-type RARα, but its biological characteristics have not been reported. This study takes two approaches. Firstly, the NLS-RARα was silenced with pNLS-RARα-shRNA. The mRNA and protein expression of NLS-RARα were detected by RT-PCR and Western blot respectively. Cell proliferation in vitro was assessed by MTT assay. Flow cytometry (FCM) was used to detect the differentiation of cells. Secondly, the NLS-RARα was over-expressed by preparation of recombinant adenovirus HL-60/pAd-NLS-RARα. The assays of mRNA and protein expression of NLS-RARα, and cell proliferation, were as above. By contrast, cell differentiation was stimulated by all trans retinoic acid (ATRA) (2.5µmol/L) at 24h after virus infection of pAd-NLS-RARα, and then detected by CD11b labeling two days later. The transcription and translation of C-MYC was detected in HL-60/pAd-NLS-RARα cells which treated by ATRA. Our results showed that compared to the control groups, the expression of NLS-RARα was significantly reduced in the HL-60/pNLS-RARα-shRNA cells, and increased dramatically in the HL-60/pAd-NLS-RARα cells. The proliferation was remarkably inhibited in the HL-60/pNLS-RARα-shRNA cells in a time-dependent manner, but markedly promoted in the HL-60/pAd-NLS-RARα cells. FCM outcome revealed the differentiation increased in HL-60/pNLS-RARα-shRNA cells, and decreased in the HL-60/pAd-NLS-RARα cells treated with 2.5µmol/L ATRA. The

Energy balance in TM-1-MH Tokamak (ohmical heating)

NASA Astrophysics Data System (ADS)

Stoeckel, J.; Koerbel, S.; Kryska, L.; Kopecky, V.; Dadalec, V.; Datlov, J.; Jakubka, K.; Magula, P.; Zacek, F.; Pereverzev, G. V.

1981-10-01

Plasma in the TM-1-MH Tokamak was experimentally studied in the parameter range: tor. mg. field B = 1,3 T, plasma current I sub p = 14 kA, electron density N sub E 3.10 to the 19th power cubic meters. The two numerical codes are available for the comparison with experimental data. TOKATA-code solves simplified energy balance equations for electron and ion components. TOKSAS-code solves the detailed energy balance of the ion component.

[Drug resistance reversal of HL-60/ADR cells by simultaneous suppression of XIAP and MRP].

PubMed

Wang, Xiao-Fang; Wang, Chun; Qin, You-Wen; Yan, Shi-Ke; Gao, Yan-Rong

2006-12-01

This study was purposed to explore the mechanisms of drug resistance of HL-60/ADR cells and to compare the reversal drug-resistance effects of antisense oligonucleotides (AS ODN) of XIAP (X-linked inhibitor of apoptosis protein) and AS ODNs of MRP (multidrug resistance-associated protein) by use alone or in combination. Reverse transcription-PCR and Western blot were applied to detect the expression of XIAP, BCL-2, MRP and MDR1 in mRNA and protein levels of HL-60 cells and HL-60/ADR cells, respectively. Fully phosphorothioated AS ODN of XIAP and MRP was delivered into HL-60/ADR cells with Lipofectamine 2000 in the form of liposome-ODN complexes alone or in combination. CCK-8 cell viability assay was used to determine the effect of AS ODN of XIAP and MRP used alone or in combination on the chemotherapy sensitivity of HL-60/ADR cells to daunorubicin (DNR). Reverse transcription-PCR and Western blot were applied to examine the changes of XIAP, MRP in mRNA and protein levels respectively. The results showed that MRP and XIAP were both significantly higher in HL-60/ADR cells than those in HL-60 cells. AS ODN of XIAP and MRP down-regulated the expression of XIAP and MRP in HL-60/ADR cells and increased the sensitivity of HL-60/ADR cells to DNR, respectively. AS ODN of XIAP + MRP did not enhance the inhibition expression of XIAP in HL-60/ADR cells but increased the sensitivity of HL-60/ADR cells to DNR significantly as compared with AS ODN of XIAP (P < 0.05). AS ODN of XIAP + MRP did not increase the concentration of DNR nor enhanced the inhibition expression of MRP in HL-60/ADR cells but increased the sensitivity of HL-60/ADR cells to DNR significantly (P < 0.05), as compared with AS ODN of MRP. It is concluded that both XIAP and MRP may be involved in the drug resistance mechanisms of HL-60/ADR cells. Drug-resistance of HL-60/ADR cells can be reversed significantly when antisense oligonucleotides of XIAP and MRP were used in combination.

Effect of coumarins on HL-60 cell differentiation.

PubMed

Kawaii, S; Tomono, Y; Katase, E; Ogawa, K; Yano, M

2000-01-01

Twenty-eight coumarins, including 7 furocoumarins, were examined for their activity of induction of terminal differentiation of human promyelocytic leukemia cells (HL-60) by nitro blue tetrazolium (NBT) reducing, nonspecific esterase, specific esterase and phagocytic activities. Esculetin, nordalbergin, 6,7-dihydroxy-4-methylcoumarin and imperatorin had strong activity among the coumarins examined. HL-60 cells treated with these coumarins differentiated into mature monocyte/macrophage. The structure-activity relationship established from the results revealed that 6,7-dihydroxy moiety had an important role in the induction of differentiation of HL-60.

Characterisation of re-entrant circuit (or rotational activity) in vitro using the HL1-6 myocyte cell line.

PubMed

Houston, Charles; Tzortzis, Konstantinos N; Roney, Caroline; Saglietto, Andrea; Pitcher, David S; Cantwell, Chris D; Chowdhury, Rasheda A; Ng, Fu Siong; Peters, Nicholas S; Dupont, Emmanuel

2018-06-01

Fibrillation is the most common arrhythmia observed in clinical practice. Understanding of the mechanisms underlying its initiation and maintenance remains incomplete. Functional re-entries are potential drivers of the arrhythmia. Two main concepts are still debated, the "leading circle" and the "spiral wave or rotor" theories. The homogeneous subclone of the HL1 atrial-derived cardiomyocyte cell line, HL1-6, spontaneously exhibits re-entry on a microscopic scale due to its slow conduction velocity and the presence of triggers, making it possible to examine re-entry at the cellular level. We therefore investigated the re-entry cores in cell monolayers through the use of fluorescence optical mapping at high spatiotemporal resolution in order to obtain insights into the mechanisms of re-entry. Re-entries in HL1-6 myocytes required at least two triggers and a minimum colony area to initiate (3.5 to 6.4 mm 2 ). After electrical activity was completely stopped and re-started by varying the extracellular K + concentration, re-entries never returned to the same location while 35% of triggers re-appeared at the same position. A conduction delay algorithm also allows visualisation of the core of the re-entries. This work has revealed that the core of re-entries is conduction blocks constituted by lines and/or groups of cells rather than the round area assumed by the other concepts of functional re-entry. This highlights the importance of experimentation at the microscopic level in the study of re-entry mechanisms. Copyright © 2018 The Authors. Published by Elsevier Ltd.. All rights reserved.

[Effect of Inhibiting and Activating Wnt Signalling Pathway on NSC67657-inducing Monocytic Differentiation of HL-60 Cells].

PubMed

Wang, Wei-Jia; Zhang, Xiu-Ming; Zhang, Yan; Wang, Jin-Shu

2016-04-01

To investigate the effect of inhibiting and activating Wnt signalling pathway on monocyte differentiation of HL-60 cells induced with a new steroidal drug NSC67657 and its possible mechamism. The HL-60 cells were treated with 5, 10 and 20 µmol/L XAV-939 (inhibitor of Wnt signalling pathway) for 3 days, and with 10, 20 and 30 mmol/L LiCl (activator of Wnt signalling pathway) for 1 day; the expression levels of down-stream genes and proteins of Wnt signolling pathway were detected by RT-PCR and Western blot, respectively; the expression of cell surface differentiation antigen CD14 and early apoptosis of HL-60 cells was detected by flow cytometry, moreover the most suitable concentration of Wnt inhibitor and activator for HL-60 cells was determined. Then the HL-60 cells with inhibited and activated Wnt pathway were treated with NSC67657 of 10 µmol/L for 3 days; the expression levels of CD14 and down-stream target proteins of Wnt signalling pathway in blank control (culture mediam) group, simple NSC67657-treated group, NSC67657 combined with inhibitor group and NSC67657 combined activator group were compared and analyzed. 20 µmol/L XAV-939 and 20 mmol/L LiCl could effectively inhibit and activate Wnt signalling pathway of HL-60 cells respectively, could significantly down- and up-regulate the expression of cyclinD1, TCF1 and c-Jun genes (P < 0.05) and proteins (P < 0.05); moreover, the number of CD10(+) HL-60 cells in these conditions was below 1%, no early apoptosis of HL-60 cells was found. In the simple NSC67657-treated groups, the expression of cyclinD1, TCF1 and c-Jun proteins was down-regulated (P < 0.05), and the percentage of CD14(+) HL-60 cells accounted for 62.13 ± 9.44; after the HL-60 cells were treated with XAV-939, the NSC67657 could more significantly down-regulate the expression of cyclinD1, TCF1 and c-Jun proteins and the percentage of CD14(+) HL-60 cell accounted for 84.17 ± 5.39%, as compared with simple NSC67657-treated group; as compared with

Implementing the HL7v3 standard in Croatian primary healthcare domain.

PubMed

Koncar, Miroslav

2004-01-01

The mission of HL7 Inc. is to provide standards for the exchange, management and integration of data that supports clinical patient care and the management, delivery and evaluation of healthcare services. The scope of this work includes the specifications of flexible, cost-effective approaches, standards, guidelines, methodologies, and related services for interoperability between healthcare information systems. In the field of medical information technologies, HL7 provides the world's most advanced information standards. Versions 1 and 2 of the HL7 standard have on the one hand solved many issues, but on the other demonstrated the size and complexity of the health information sharing problem. As the solution, a complete new methodology has been adopted, which is being encompassed in version 3 recommendations. This approach standardizes the Reference Information Model (RIM), which is the source of all domain models and message structures. Message design is now defined in detail, enabling interoperability between loosely-coupled systems that are designed by different vendors and deployed in various environments. At the start of the Primary Healthcare Information System project, we have decided to go directly to HL7v3. Implementing the HL7v3 standard in healthcare applications represents a challenging task. By using standardized refinement and localization methods we were able to define information models for Croatian primary healthcare domain. The scope of our work includes clinical, financial and administrative data management, where in some cases we were compelled to introduce new HL7v3-compliant models. All of the HL7v3 transactions are digitally signed, using the W3C XML Digital Signature standard.

Edge profile measurements using Thomson scattering on the KSTAR tokamak

DOE Office of Scientific and Technical Information (OSTI.GOV)

Lee, J. H., E-mail: jhleel@nfri.re.kr; Ko, W. H.; Department of Nuclear Fusion and Plasma Science, University of Science and Technology

2014-11-15

In the KSTAR Tokamak, a “Tangential Thomson Scattering” (TTS) diagnostic system has been designed and installed to measure electron density and temperature profiles. In the edge system, TTS has 12 optical fiber bundles to measure the edge profiles with 10–15 mm spatial resolution. These 12 optical fibers and their spatial resolution are not enough to measure the pedestal width with a high accuracy but allow observations of L-H transition or H-L transitions at the edge. For these measurements, the prototype ITER edge Thomson Nd:YAG laser system manufactured by JAEA in Japan is installed. In this paper, the KSTAR TTS systemmore » is briefly described and some TTS edge profiles are presented and compared against the KSTAR Charge Exchange Spectroscopy and other diagnostics. The future upgrade plan of the system is also discussed in this paper.« less

Magnetic evaluation of hydrogen pressures changes on MHD fluctuations in IR-T1 tokamak plasma

NASA Astrophysics Data System (ADS)

Alipour, Ramin; Ghanbari, Mohamad R.

2018-04-01

Identification of tokamak plasma parameters and investigation on the effects of each parameter on the plasma characteristics is important for the better understanding of magnetohydrodynamic (MHD) activities in the tokamak plasma. The effect of different hydrogen pressures of 1.9, 2.5 and 2.9 Torr on MHD fluctuations of the IR-T1 tokamak plasma was investigated by using of 12 Mirnov coils, singular value decomposition and wavelet analysis. The parameters such as plasma current, loop voltage, power spectrum density, energy percent of poloidal modes, dominant spatial structures and temporal structures of poloidal modes at different plasma pressures are plotted. The results indicate that the MHD activities at the pressure of 2.5 Torr are less than them at other pressures. It also has been shown that in the stable area of plasma and at the pressure of 2.5 Torr, the magnetic force and the force of plasma pressure are in balance with each other and the MHD activities are at their lowest level.

Radioactivity evaluation for the KSTAR tokamak.

PubMed

Kim, Hyunduk; Lee, Hee-Seock; Hong, Sukmo; Kim, Minho; Chung, Chinwha; Kim, Changsuk

2005-01-01

The deuterium-deuterium (D-D) reaction in the KSTAR (Korea Superconducting Tokamak Advanced Research) tokamak generates neutrons with a peak yield of 2.5 x 10(16) s(-1) through a pulse operation of 300 s. Since the structure material of the tokamak is irradiated with neutrons, this environment will restrict work around and inside the tokamak from a radiation protection physics point of view after shutdown. Identification of neutron-produced radionuclides and evaluation of absorbed dose in the structure material are needed to develop a guiding principle for radiation protection. The activation level was evaluated by MCNP4C2 and an inventory code, FISPACT. The absorbed dose in the working area decreased by 4.26 x 10(-4) mrem h(-1) in the inner vessel 1.5 d after shutdown. Furthermore, tritium strongly contributes to the contamination in the graphite tile. The amount of tritium produced by neutrons was 3.03 x 10(6) Bq kg(-1) in the carbon graphite of a plasma-facing wall.

HL-10 on lakebed with Jerauld R. Gentry, Peter Hoag, John A. Manke, and Bill Dana

NASA Technical Reports Server (NTRS)

1969-01-01

The four principal HL-10 pilots are seen here with the lifting body aircraft. They are, left to right; Air Force Major Jerauld R. Gentry, Air Force test pilot Peter Hoag, and NASA pilots John A. Manke and Bill Dana. All are wearing the pressure suits needed for flying above 50,000 feet. The HL-10 was one of five heavyweight lifting-body designs flown at NASA's Flight Research Center (FRC--later Dryden Flight Research Center), Edwards, California, from July 1966 to November 1975 to study and validate the concept of safely maneuvering and landing a low lift-over-drag vehicle designed for reentry from space. Northrop Corporation built the HL-10 and M2-F2, the first two of the fleet of 'heavy' lifting bodies flown by the NASA Flight Research Center. The contract for construction of the HL-10 and the M2-F2 was $1.8 million. 'HL' stands for horizontal landing, and '10' refers to the tenth design studied by engineers at NASA's Langley Research Center, Hampton, Va. After delivery to NASA in January 1966, the HL-10 made its first flight on Dec. 22, 1966, with research pilot Bruce Peterson in the cockpit. Although an XLR-11 rocket engine was installed in the vehicle, the first 11 drop flights from the B-52 launch aircraft were powerless glide flights to assess handling qualities, stability, and control. In the end, the HL-10 was judged to be the best handling of the three original heavy-weight lifting bodies (M2-F2/F3, HL-10, X-24A). The HL-10 was flown 37 times during the lifting body research program and logged the highest altitude and fastest speed in the Lifting Body program. On Feb. 18, 1970, Air Force test pilot Peter Hoag piloted the HL-10 to Mach 1.86 (1,228 mph). Nine days later, NASA pilot Bill Dana flew the vehicle to 90,030 feet, which became the highest altitude reached in the program. Some new and different lessons were learned through the successful flight testing of the HL-10. These lessons, when combined with information from it's sister ship, the M2-F2/F3

Retinoic Acid Therapy Resistance Progresses from Unilineage to Bilineage in HL-60 Leukemic Blasts

PubMed Central

Jensen, Holly A.; Bunaciu, Rodica P.; Ibabao, Christopher N.; Myers, Rebecca; Varner, Jeffrey D.; Yen, Andrew

2014-01-01

Emergent resistance can be progressive and driven by global signaling aberrations. All-trans retinoic acid (RA) is the standard therapeutic agent for acute promyelocytic leukemia, but 10–20% of patients are not responsive, and initially responsive patients relapse and develop retinoic acid resistance. The patient-derived, lineage-bipotent acute myeloblastic leukemia (FAB M2) HL-60 cell line is a potent tool for characterizing differentiation-induction therapy responsiveness and resistance in t(15;17)-negative cells. Wild-type (WT) HL-60 cells undergo RA-induced granulocytic differentiation, or monocytic differentiation in response to 1,25-dihydroxyvitamin D3 (D3). Two sequentially emergent RA-resistant HL-60 cell lines, R38+ and R38-, distinguishable by RA-inducible CD38 expression, do not arrest in G1/G0 and fail to upregulate CD11b and the myeloid-associated signaling factors Vav1, c-Cbl, Lyn, Fgr, and c-Raf after RA treatment. Here, we show that the R38+ and R38- HL-60 cell lines display a progressive reduced response to D3-induced differentiation therapy. Exploiting the biphasic dynamic of induced HL-60 differentiation, we examined if resistance-related defects occurred during the first 24 h (the early or “precommitment” phase) or subsequently (the late or “lineage-commitment” phase). HL-60 were treated with RA or D3 for 24 h, washed and retreated with either the same, different, or no differentiation agent. Using flow cytometry, D3 was able to induce CD38, CD11b and CD14 expression, and G1/G0 arrest when present during the lineage-commitment stage in R38+ cells, and to a lesser degree in R38- cells. Clustering analysis of cytometry and quantified Western blot data indicated that WT, R38+ and R38- HL-60 cells exhibited decreasing correlation between phenotypic markers and signaling factor expression. Thus differentiation induction therapy resistance can develop in stages, with initial partial RA resistance and moderate vitamin D3 responsiveness

Uric acid disrupts hypochlorous acid production and the bactericidal activity of HL-60 cells.

PubMed

Carvalho, Larissa A C; Lopes, João P P B; Kaihami, Gilberto H; Silva, Railmara P; Bruni-Cardoso, Alexandre; Baldini, Regina L; Meotti, Flavia C

2018-06-01

Uric acid is the end product of purine metabolism in humans and is an alternative physiological substrate for myeloperoxidase. Oxidation of uric acid by this enzyme generates uric acid free radical and urate hydroperoxide, a strong oxidant and potentially bactericide agent. In this study, we investigated whether the oxidation of uric acid and production of urate hydroperoxide would affect the killing activity of HL-60 cells differentiated into neutrophil-like cells (dHL-60) against a highly virulent strain (PA14) of the opportunistic pathogen Pseudomonas aeruginosa. While bacterial cell counts decrease due to dHL-60 killing, incubation with uric acid inhibits this activity, also decreasing the release of the inflammatory cytokines interleukin-1β (IL-1β) and tumor necrosis factor-α (TNF- α). In a myeloperoxidase/Cl - /H 2 O 2 cell-free system, uric acid inhibited the production of HOCl and bacterial killing. Fluorescence microscopy showed that uric acid also decreased the levels of HOCl produced by dHL-60 cells, while significantly increased superoxide production. Uric acid did not alter the overall oxidative status of dHL-60 cells as measured by the ratio of reduced (GSH) and oxidized (GSSG) glutathione. Our data show that uric acid impairs the killing activity of dHL-60 cells likely by competing with chloride by myeloperoxidase catalysis, decreasing HOCl production. Despite diminishing HOCl, uric acid probably stimulates the formation of other oxidants, maintaining the overall oxidative status of the cells. Altogether, our results demonstrated that HOCl is, indeed, the main relevant oxidant against bacteria and deviation of myeloperoxidase activity to produce other oxidants hampers dHL-60 killing activity. Copyright © 2018 The Authors. Published by Elsevier B.V. All rights reserved.

42GHz ECRH assisted Plasma Breakdown in tokamak SST-1

NASA Astrophysics Data System (ADS)

Shukla, B. K.; Pradhan, S.; Patel, Paresh; Babu, Rajan; Patel, Jatin; Patel, Harshida; Dhorajia, Pragnesh; Tanna, V.; Atrey, P. K.; Manchanda, R.; Gupta, Manoj; Joisa, Shankar; Gupta, C. N.; Danial, Raju; Singh, Prashant; Jha, R.; Bora, D.

2015-03-01

In SST-1, 42GHz ECRH system has been commissioned to carry out breakdown and heating experiments at 0.75T and 1.5T operating toroidal magnetic fields. The 42GHz ECRH system consists of high power microwave source Gyrotron capable to deliver 500kW microwave power for 500ms duration, approximately 20 meter long transmission line and a mirror based launcher. The ECRH power in fundamental O-mode & second harmonic X-mode is launched from low field side (radial port) of the tokamak. At 0.75T operation, approximately 300 kW ECH power is launched in second harmonic X-mode and successful ECRH assisted breakdown is achieved at low loop_voltage ~ 3V. The ECRH power is launched around 45ms prior to loop voltage. The hydrogen pressure in tokamak is maintained ~ 1×10-5mbar and the pre-ionized density is ~ 4×1012/cc. At 1.5T operating toroidal magnetic field, the ECH power is launched in fundamental O-mode. The ECH power at fundamental harmonic is varied from 100 kW to 250 kW and successful breakdown is achieved in all ECRH shots. In fundamental harmonic there is no delay in breakdown while at second harmonic ~ 40ms delay is observed, which is normal in case of second harmonic ECRH assisted breakdown.

Structure of chaotic magnetic field lines in IR-T1 tokamak due to ergodic magnetic limiter

NASA Astrophysics Data System (ADS)

Ahmadi, S.; Salar Elahi, A.; Ghorannevis, M.

2018-03-01

In this paper we have studied an Ergodic Magnetic Limiter (EML) based chaotic magnetic field for transport control in the edge plasma of IR-T1 tokamak. The resonance created by the EML causes perturbation of the equilibrium field line in tokamak and as a result, the field lines are chaotic in the vicinity of the dimerized island chains. Transport barriers are formed in the chaotic field line and actually observe in tokamak with reverse magnetic shear. We used area-preserving non-twist (and twist) Poincaré maps to describe the formation of transport barriers, which are actually features of Hamiltonian systems. This transport barrier is useful in reducing radial diffusion of the field line and thus improving the plasma confinement.

Understanding and predicting the dynamics of tokamak discharges during startup and rampdown

DOE Office of Scientific and Technical Information (OSTI.GOV)

Jackson, G. L.; Politzer, P. A.; Humphreys, D. A.

Understanding the dynamics of plasma startup and termination is important for present tokamaks and for predictive modeling of future burning plasma devices such as ITER. We report on experiments in the DIII-D tokamak that explore the plasma startup and rampdown phases and on the benchmarking of transport models. Key issues have been examined such as plasma initiation and burnthrough with limited inductive voltage and achieving flattop and maximum burn within the technical limits of coil systems and their actuators while maintaining the desired q profile. Successful rampdown requires scenarios consistent with technical limits, including controlled H-L transitions, while avoiding verticalmore » instabilities, additional Ohmic transformer flux consumption, and density limit disruptions. Discharges were typically initiated with an inductive electric field typical of ITER, 0.3 V/m, most with second harmonic electron cyclotron assist. A fast framing camera was used during breakdown and burnthrough of low Z impurity charge states to study the formation physics. An improved 'large aperture' ITER startup scenario was developed, and aperture reduction in rampdown was found to be essential to avoid instabilities. Current evolution using neoclassical conductivity in the CORSICA code agrees with rampup experiments, but the prediction of the temperature and internal inductance evolution using the Coppi-Tang model for electron energy transport is not yet accurate enough to allow extrapolation to future devices.« less

Differential effect of 1{alpha},25-dihydroxyvitamin D{sub 3} on Hsp28 and PKC{beta} gene expression in the phorbol ester-resistant human myeloid HL-525 leukemic cells

DOE Office of Scientific and Technical Information (OSTI.GOV)

Lee, Yong J.; Galoforo, S.S.; Berns, C.M.

We investigated the effect of 1{alpha},25-dihydroxyvitamin D{sub 3} [1,25-(OH){sub 2}D{sub 3}] on the expression of the 28-kDa heat shock protein gene (hsp28) and the protein kinase C beta gene (PKC{beta}) in the human myeloid HL-60 leukemic cell variant HL-525, which is resistance to phorbol ester-induced macrophage differentiation. Northern and Western blot analysis showed little or no hsp28 gene expression in the HL-60 cell variant, HL-205, which is susceptible to such differentiation, while a relatively high basal level of hps28 gene expression was observed in the HL-525 cells. However, both cell lines demonstrated heat shock-induced expression of this gene. During treatmentmore » with 50-300 nM 1,25-(OH){sub 2}D{sub 3}, a marked reduction of hsp28 gene expression was not associated with heat shock transcription factor-heat shock element (HSF-HSE) binding activity. Our results suggest that the differential effect of 1,25-(OH){sub 2}D{sub 3} on hsp28 and PKC{beta} gene expression is due to the different sequence composition of the vitamin D response element in the in the promoter region as well as an accessory factor for each gene or that 1,25-(OH){sub 2}D{sub 3} increases PKC{beta} gene expression, which in turn negatively regulates the expression of the hsp28 gene, or vice versa.« less

Reactions Involved in the Lower Pathway for Degradation of 4-Nitrotoluene by Mycobacterium Strain HL 4-NT-1

PubMed Central

He, Zhongqi; Spain, Jim C.

2000-01-01

In spite of the variety of initial reactions, the aerobic biodegradation of aromatic compounds generally yields dihydroxy intermediates for ring cleavage. Recent investigation of the degradation of nitroaromatic compounds revealed that some nitroaromatic compounds are initially converted to 2-aminophenol rather than dihydroxy intermediates by a number of microorganisms. The complete pathway for the metabolism of 2-aminophenol during the degradation of nitrobenzene by Pseudomonas pseudoalcaligenes JS45 has been elucidated previously. The pathway is parallel to the catechol extradiol ring cleavage pathway, except that 2-aminophenol is the ring cleavage substrate. Here we report the elucidation of the pathway of 2-amino-4-methylphenol (6-amino-m-cresol) metabolism during the degradation of 4-nitrotoluene by Mycobacterium strain HL 4-NT-1 and the comparison of the substrate specificities of the relevant enzymes in strains JS45 and HL 4-NT-1. The results indicate that the 2-aminophenol ring cleavage pathway in strain JS45 is not unique but is representative of the pathways of metabolism of other o-aminophenolic compounds. PMID:10877799

Tokamak foundation in USSR/Russia 1950-1990

NASA Astrophysics Data System (ADS)

Smirnov, V. P.

2010-01-01

In the USSR, nuclear fusion research began in 1950 with the work of I.E. Tamm, A.D. Sakharov and colleagues. They formulated the principles of magnetic confinement of high temperature plasmas, that would allow the development of a thermonuclear reactor. Following this, experimental research on plasma initiation and heating in toroidal systems began in 1951 at the Kurchatov Institute. From the very first devices with vessels made of glass, porcelain or metal with insulating inserts, work progressed to the operation of the first tokamak, T-1, in 1958. More machines followed and the first international collaboration in nuclear fusion, on the T-3 tokamak, established the tokamak as a promising option for magnetic confinement. Experiments continued and specialized machines were developed to test separately improvements to the tokamak concept needed for the production of energy. At the same time, research into plasma physics and tokamak theory was being undertaken which provides the basis for modern theoretical work. Since then, the tokamak concept has been refined by a world-wide effort and today we look forward to the successful operation of ITER.

Use of HL cells for improved isolation and passage of Chlamydia pneumoniae.

PubMed Central

Cles, L D; Stamm, W E

1990-01-01

We compared growth of the recently discovered respiratory pathogen Chlamydia pneumoniae in McCoy, HeLa 229, BHK-21, and HL cells. When cells were not pretreated with DEAE-dextran, HL cells had significantly higher mean numbers of inclusion-forming units (IFUs) on initial inoculation than the other cell lines. When cells were pretreated with DEAE-dextran, HeLa 229 and HL cells had equivalent mean numbers of IFUs on initial inoculation. HL cells had strikingly higher mean numbers of IFUs in passage than HeLa 229, BHK-21, or McCoy cells. In addition, HL cells did not require pretreatment with DEAE-dextran and could be used from 2 to 4 days after seeding. We conclude that HL cells are an excellent cell culture system for laboratory propagation of C. pneumoniae and may be a more sensitive cell line for initial isolation. PMID:2191013

Differentiation of HL-60 cells distinguishes between cytostatic and cytotoxic effects of the alkylphospholipid ET-18-OCH3.

PubMed

Civoli, F; Pauig, S B; Daniel, L W

1996-01-01

The synthetic dialkylphospholipid 1-O-octadecyl-2-O-methyl-rac-glycero-3-phosphocholine (ET-18-OCH3) inhibits growth of the acute myelogenous leukemia cell line HL-60. Incubation of HL-60 cells with demethyl-sulfoxide causes the cells to differentiate to a granulocyte-like phenotype and become quiescent. Incubation of the DMSO-treated cells with ET-18-OCH3 results in a reduction in cell numbers due to cytotoxicity. In contrast, treatment of undifferentiated HL-60 cells with lower concentrations of ET-18-OCH3 leads to growth inhibition. These data indicate that the model of differentiated HL-60 cells currently used for the study of resistance to growth inhibition is inappropriate. HL-60 cells can be used to measure growth inhibition and at higher doses cytotoxicity. However, the differentiated, nonproliferative, cells can only be used to measure direct cytotoxicity. Therefore, the results of studies directly comparing the effects of ET-18-OCH3 in proliferative HL-60 cells and quiescent DMSO-treated HL-60 cells should be reevaluated. An evaluation of the effects of low concentrations of ET-18-OCH3 (0.5-1.5 microM) in proliferative HL-60 cells indicated that ET-18-OCH3 was an effective cytostatic agent at nontoxic concentrations. In summary, studies on the mechanism of action of ET-18-OCH3, or related ether lipids, should carefully investigate differences in the effects at cytostatic versus cytotoxic concentrations.

Bill Dana in front of HL-10 after flight H-24-37

NASA Image and Video Library

1969-09-03

NASA research pilot Bill Dana after his fourth free flight (1 glide and 3 powered) in the HL-10. This particular flight reached a maximum speed of Mach 1.45. Dana made a total of nine HL-10 flights (1 glide and 8 powered), and his lifting body experience as a whole included several car tow and 1 air tow flights in the M2-F1; 4 glide and 15 powered flights in the M2-F3; and 2 powered flights in the X-24B. He is wearing a pressure suit for protection against the cockpit depressurizing at high altitudes. The air conditioner box held by the ground crewman provides cool air to prevent overheating.

The X-15/HL-20 operations support comparison

NASA Technical Reports Server (NTRS)

Morris, W. Douglas

1993-01-01

During the 1960's, the United States X-15 rocket-plane research program successfully demonstrated the ability to support a reusable vehicle operating in a near-space environment. The similarity of the proposed HL-20 lifting body concept in general size, weight, and subsystem composition to that of the X-15 provided an opportunity for a comparison of the predicted support manpower and turnaround times with those experienced in the X-15 program. Information was drawn from both reports and discussions with X-15 program personnel to develop comparative operations and support data. Based on the assumption of comparability between the two systems, the predicted staffing levels, skill mix, and refurbishment times of an operational HL-20 appear to be similar to those experienced by the X-15 for ground support. However, safety, environmental, and support requirements have changed such that the HL-20 will face a different operating environment than existed at Edwards during the 1950's and 1960's. Today's operational standards may impose additional requirements on the HL-20 that will add to the maintenance and support burden estimate based on the X-15 analogy.

Gossypol inhibits phosphorylation of Bcl-2 in human leukemia HL-60 cells.

PubMed

Huang, Li-heng; Hu, Jia-qi; Tao, Wei-qun; Li, Yuan-hong; Li, Guan-ming; Xie, Pei-yi; Liu, Xiao-shan; Jiang, Jikai

2010-10-25

Gossypol is an attractive therapeutic anti-tumor agent as an apoptosis inducer and is being evaluated in preclinical tests. However, the molecular mechanisms underlying apoptosis induction by gossypol in malignant cells have not been completely enunciated. Here we investigate the alterations of Bcl-2/Bcl-xL/Mcl-1 protein levels and Bcl-2 phosphorylation in gossypol-induced apoptosis in human leukemia HL-60 cells. We found that gossypol treatment inhibited cell growth and induced apoptosis in HL-60 cells. Bcl-2/Bcl-xL/Mcl-1 protein levels were slightly reduced and phosphorylation of Bcl-2 at threonine 56 (phospho T56) was not altered. However, phosphorylation of Bcl-2 at serine 70 (phospho S70) was strikingly down-regulated in gossypol-exposed cells. This reduction was found to be not only in both dose- and time-dependent fashion but also obviated by phorbol l2,13-dibutyrate (PDBu), an activator of protein kinase C (PKC). In addition, pre-treatment of PDBu partially prevented gossypol-induced apoptosis in HL-60 cells. Collectively, gossypol treatment can reduce phosphorylation of Bcl-2 at serine 70 in leukemia HL-60 cells and gossypol may be a promising therapeutical candidate for leukemia patients especially expressing phosphorylated Bcl-2 at Ser70. Copyright 2010 Elsevier B.V. All rights reserved.

Status of the tokamak program

NASA Astrophysics Data System (ADS)

Sheffield, J.

1981-08-01

For a specific configuration of magnetic field and plasma to be economically attractive as a commercial source of energy, it must contain a high-pressure plasma in a stable fashion while thermally isolating the plasma from the walls of the containment vessel. The tokamak magnetic configuration is presently the most successful in terms of reaching the considered goals. Tokamaks were developed in the USSR in a program initiated in the mid-1950s. By the early 1970s tokamaks were operating not only in the USSR but also in the U.S., Australia, Europe, and Japan. The advanced state of the tokamak program is indicated by the fact that it is used as a testbed for generic fusion development - for auxiliary heating, diagnostics, materials - as well as for specific tokamak advancement. This has occurred because it is the most economic source of a large, reproducible, hot, dense plasma. The basic tokamak is considered along with tokamak improvements, impurity control, additional heating, particle and power balance in a tokamak, aspects of microscopic transport, and macroscopic stability.

Cytotoxicity and cellular uptake of doxorubicin and its formamidine derivatives in HL60 sensitive and HL60/MX2 resistant cells.

PubMed

Kik, Krzysztof; Wasowska-Lukawska, Malgorzata; Oszczapowicz, Irena; Szmigiero, Leszek

2009-04-01

In this work a comparison was made of the cytotoxicity and cellular uptake of doxorubicin (DOX) and two of its derivatives containing a formamidino group (-N=CH-N<) at the 3' position with morpholine (DOXM) or hexamethyleneimine (DOXH) ring. All tests were performed in doxorubicin-sensitive HL60 and -resistant HL60/MX2 cells which are known for the presence of altered topoisomerase II. Cytotoxic activity of DOX toward HL60/MX2 cells was about 195 times lower when compared with the sensitive HL60 cell line. DOXM and DOXH were approximately 20 times more active in resistant cells than DOX. It was found that the uptake of DOX was lower in resistant cells by about 16%, while that of DOXM and DOXH was lower by about 36% and 19%, respectively. Thus the changes in the cellular uptake of anthracyclines are not associated with the fact that cytotoxicity of DOXM and DOXH exceed the cytotoxicity of DOX. Experiments in cell-free system containing human topoisomerase II showed that topoisomerase II is not inhibited by DOXM and DOXH. Formamidinoanthracyclines may be more useful than parent drugs in therapy against tumor cells with altered topoisomerase II activity.

Application of automatic gain control for radiometer diagnostic in SST-1 tokamak.

PubMed

Makwana, Foram R; Siju, Varsha; Edappala, Praveenlal; Pathak, S K

2017-12-01

This paper describes the characterisation of a negative feedback type of automatic gain control (AGC) circuit that will be an integral part of the heterodyne radiometer system operating at a frequency range of 75-86 GHz at SST-1 tokamak. The developed AGC circuit is a combination of variable gain amplifier and log amplifier which provides both gain and attenuation typically up to 15 dB and 45 dB, respectively, at a fixed set point voltage and it has been explored for the first time in tokamak radiometry application. The other important characteristics are that it exhibits a very fast response time of 390 ns to understand the fast dynamics of electron cyclotron emission and can operate at very wide input RF power dynamic range of around 60 dB that ensures signal level within the dynamic range of the detection system.

Synergistic growth inhibition in HL-60 cells by the combination of acyclic retinoid and vitamin K2.

PubMed

Kitagawa, Junichi; Hara, Takeshi; Tsurumi, Hisashi; Ninomiya, Soranobu; Ogawa, Kengo; Adachi, Seiji; Kanemura, Nobuhiro; Kasahara, Senji; Shimizu, Masahito; Moriwaki, Hisataka

2011-05-01

The aim of this study was to assess the effects of acyclic retinoid (ACR) and vitamin K(2) (VK(2)) in HL-60 cells. We used HL-60 cells, and the Trypan Blue dye exclusion method was used for cell proliferation assays. For detection of apoptosis, the Annexin V-binding capacity of treated cells was examined by flow cytometry. To evaluate the cell cycle, we used a FITC BrdU Flow KIT and flow cytometry. Total extracted and equivalent amounts of protein were examined by Western blotting using specific antibodies. ACR and VK(2) dose dependently inhibited the proliferation of HL-60 cells. These two agents in combination synergistically inhibited cell growth and induced apoptosis. VK(2) inhibited activation of the Ras/MAPK signaling pathway, and ACR plus VK(2) cooperatively inhibited phosphorylation of RXRα and the growth of HL-60 cells. Moreover, ACR and VK(2) induced increases in G0/G1 phase HL-60 cells, alone and synergistically in combination. The synergistic effects of ACR and VK(2) on HL-60 cells may provide a novel strategy for treating leukemia.

Connecting HL Tau to the observed exoplanet sample

NASA Astrophysics Data System (ADS)

Simbulan, Christopher; Tamayo, Daniel; Petrovich, Cristobal; Rein, Hanno; Murray, Norman

2017-08-01

The Atacama Large Millimeter/submilimeter Array (ALMA) recently revealed a set of nearly concentric gaps in the protoplanetary disc surrounding the young star HL Tauri (HL Tau). If these are carved by forming gas giants, this provides the first set of orbital initial conditions for planets as they emerge from their birth discs. Using N-body integrations, we have followed the evolution of the system for 5 Gyr to explore the possible outcomes. We find that HL Tau initial conditions scaled down to the size of typically observed exoplanet orbits naturally produce several populations in the observed exoplanet sample. First, for a plausible range of planetary masses, we can match the observed eccentricity distribution of dynamically excited radial velocity giant planets with eccentricities >0.2. Secondly, we roughly obtain the observed rate of hot Jupiters around FGK stars. Finally, we obtain a large efficiency of planetary ejections of ≈2 per HL Tau-like system, but the small fraction of stars observed to host giant planets makes it hard to match the rate of free-floating planets inferred from microlensing observations. In view of upcoming Gaia results, we also provide predictions for the expected mutual inclination distribution, which is significantly broader than the absolute inclination distributions typically considered by previous studies.

[((H)L)2Fe6(NCMe)m]n+ (m = 0, 2, 4, 6; n = -1, 0, 1, 2, 3, 4, 6): an electron-transfer series featuring octahedral Fe6 clusters supported by a hexaamide ligand platform.

PubMed

Zhao, Qinliang; Harris, T David; Betley, Theodore A

2011-06-01

Using a trinucleating hexaamide ligand platform, the all-ferrous hexanuclear cluster ((H)L)(2)Fe(6) (1) is obtained from reaction of 3 equiv of Fe(2)(Mes)(4) (Mes = 2,4,6-Me(3)C(6)H(2)) with 2 equiv of the ligand ((H)L)H(6). Compound 1 was characterized by X-ray diffraction analysis, (57)Fe Mössbauer, SQUID magnetometry, mass spectrometry, and combustion analysis, providing evidence for an S=6 ground state and delocalized electronic structure. The cyclic voltammogram of [((H)L)(2)Fe(6)](n+) in acetonitrile reveals a rich redox chemistry, featuring five fully reversible redox events that span six oxidation states ([((H)L)(2)Fe(6)](n+), where n=-1→4) within a 1.3 V potential range. Accordingly, each of these species is readily accessed chemically to provide the electron-transfer series [((H)L)(2)Fe(6)(NCMe)(m)][PF(6)](n) (m=0, n=-1 (2); m=2, n=1 (3); m=4, n=2 (4); m=6, n=3 (5); m=6, n=4 (6)). Compounds 2-6 were isolated and characterized by X-ray diffraction, (57)Fe Mössbauer and multinuclear NMR spectroscopy, and combustion analysis. Two-electron oxidation of the tetracationic cluster in 6 by 2 equiv of [NO](+) generates the thermally unstable hexacationic cluster [((H)L)(2)Fe(6)(NCMe)(m)](6+), which is characterized by NMR and (57)Fe Mössbauer spectroscopy. Importantly, several stepwise systematic metrical changes accompany oxidation state changes to the [Fe(6)] core, namely trans ligation of solvent molecules and variation in Mössbauer spectra, spin ground state, and intracluster Fe-Fe separation. The observed metrical changes are rationalized by considering a qualitative, delocalized molecular orbital description, which provides a set of frontier orbitals populated by Fe 3d electrons. © 2011 American Chemical Society

Relationship of calcitonin mRNA expression to the differentiation state of HL 60 cells.

PubMed

Kiefer, P; Bacher, M; Pflüger, K H

1994-05-01

Raised plasma levels of immunoreactive human calcitonin (ihCT) can be found in patients with myeloid leukemia and seem to indicate a poor prognosis. High levels were found in acute undifferentiated and acute myeloblastic leukemia. To test whether CT expression could be a marker of myeloid differentiation, we used the promyelocytic leukemia cell line HL 60 which also expresses ihCT as a model system for myeloid differentiation. Exponentially growing HL 60 cells as well as differentiation induced HL 60 cells expressed a single 1.0 Kb CT transcript. The induction of HL 60 cell differentiation along the granulocytic lineage by DMSO or HMBA had no effect on the level of CT transcripts. Induction of monocytic/macrophagic differentiation by TPA resulted in a transient, about 10-fold elevated expression of CT steady state mRNA after 24 h. In contrast to TPA, induction of HL 60 cell differentiation along the monocytic pathway by Vit D3 had no detectable effect on the level of the CT in RNA expression at corresponding time points. These findings suggest that the transient induction of CT steady state mRNA expression by TPA is rather a direct effect of the phorbol ester than commitment along the monocytic line of differentiation.

Ultrastructural localization of human HL-A membrane antigens by use of hybrid antibodies

PubMed Central

Neauport-Sautes, Catherine; Silvestre, Daniele; Niccolai, Marie-Gabrielle; Kourilsky, F. M.; Levy, J. P.

1972-01-01

The localization of HL-A histocompatibility antigens at the surface of human lymphocytes in electron microscopy has been studied using hybrid antibodies to bind electron-dense particles (ferritin and plant viruses) to anti-HL-A antibody. A discontinuous distribution of the markers is observed at the cell surface, which is identical with that described for H-2 antigens on mouse lymphocytes with the same technique. Double labelling experiments suggest that the areas of the cell surface where HL-A antigens are detected contain also the heterologous lymphocyte antigens detected by an anti-thymocyte serum and that HL-A antigens are not renewed at a detectable level during the period of the labelling procedure in the areas of the cell surface which are not labelled primarily with ferritin-anti-IgG-anti-HL-A complexes. The interpretation of the discontinuous labelling of HL-A antigens with direct immunoferritin techniques is discussed. ImagesFIG. 2FIG. 3FIG. 4FIG. 5 PMID:5063188

In vitro antileukaemic activity of extracts from chokeberry (Aronia melanocarpa [Michx] Elliott) and mulberry (Morus alba L.) leaves against sensitive and multidrug resistant HL60 cells.

PubMed

Skupień, Katarzyna; Kostrzewa-Nowak, Dorota; Oszmiański, Jan; Tarasiuk, Jolanta

2008-05-01

The aim of the present study was to determine in vitro antileukaemic activities of extracts obtained from chokeberry (Aronia melanocarpa [Michx] Elliot) and mulberry (Morus alba L.) leaves against promyelocytic HL60 cell line and its multidrug resistant sublines exhibiting two different MDR phenotypes: HL60/VINC (overexpressing P-glycoprotein) and HL60/DOX (overexpressing MRP1 protein). It was found that the extracts from chokeberry and mulberry leaves were active against the sensitive leukaemic cell line HL60 and retained the in vitro activity against multidrug resistant sublines (HL60/VINC and HL60/DOX). The values of resistance factor (RF) found for these extracts were very low lying in the range 1.2-1.6.

ALMA Reveals Transition of Polarization Pattern with Wavelength in HL Tau’s Disk

NASA Astrophysics Data System (ADS)

Stephens, Ian W.; Yang, Haifeng; Li, Zhi-Yun; Looney, Leslie W.; Kataoka, Akimasa; Kwon, Woojin; Fernández-López, Manuel; Hull, Charles L. H.; Hughes, Meredith; Segura-Cox, Dominique; Mundy, Lee; Crutcher, Richard; Rao, Ramprasad

2017-12-01

The mechanism for producing polarized emission from protostellar disks at (sub)millimeter wavelengths is currently uncertain. Classically, polarization is expected from non-spherical grains aligned with the magnetic field. Recently, two alternatives have been suggested. One polarization mechanism is caused by self-scattering from dust grains of sizes comparable with the wavelength, while the other mechanism is due to grains aligned with their short axes along the direction of radiation anisotropy. The latter has recently been shown as a likely mechanism for causing the dust polarization detected in HL Tau at 3.1 mm. In this paper, we present ALMA polarization observations of HL Tau for two more wavelengths: 870 μm and 1.3 mm. The morphology at 870 μm matches the expectation for self-scattering, while that at 1.3 mm shows a mix between self-scattering and grains aligned with the radiation anisotropy. The observations cast doubt on the ability of (sub)millimeter continuum polarization to probe disk magnetic fields for at least HL Tau. By showing two distinct polarization morphologies at 870 μm and 3.1 mm and a transition between the two at 1.3 mm, this paper provides definitive evidence that the dominant (sub)millimeter polarization mechanism transitions with wavelength. In addition, if the polarization at 870 μm is due to scattering, the lack of polarization asymmetry along the minor axis of the inclined disk implies that the large grains responsible for the scattering have already settled into a geometrically thin layer, and the presence of asymmetry along the major axis indicates that the HL Tau disk is not completely axisymmetric.

X-15A-2 and HL-10 parked on NASA ramp

NASA Technical Reports Server (NTRS)

1966-01-01

Both the HL-10 and X-15A2, shown here parked beside one another on the NASA ramp in 1966, underwent modifications. The X-15 No. 2 had been damaged in a crash landing in November 1962. Subsequently, the fuselage was lengthened, and it was outfitted with two large drop tanks. These modifications allowed the X-15A-2 to reach the speed of Mach 6.7. On the HL-10, the stability problems that appeared on the first flight at the end of 1966 required a reshaping of the fins' leading edges to eliminate the separated airflow that was causing the unstable flight. By cambering the leading edges of the fins, the HL-10 team achieved attached flow and stable flight. The HL-10 was one of five heavyweight lifting-body designs flown at NASA's Flight Research Center (FRC--later Dryden Flight Research Center), Edwards, California, from July 1966 to November 1975 to study and validate the concept of safely maneuvering and landing a low lift-over-drag vehicle designed for reentry from space. Northrop Corporation built the HL-10 and M2-F2, the first two of the fleet of 'heavy' lifting bodies flown by the NASA Flight Research Center. The contract for construction of the HL-10 and the M2-F2 was $1.8 million. 'HL' stands for horizontal landing, and '10' refers to the tenth design studied by engineers at NASA's Langley Research Center, Hampton, Va. After delivery to NASA in January 1966, the HL-10 made its first flight on Dec. 22, 1966, with research pilot Bruce Peterson in the cockpit. Although an XLR-11 rocket engine was installed in the vehicle, the first 11 drop flights from the B-52 launch aircraft were powerless glide flights to assess handling qualities, stability, and control. In the end, the HL-10 was judged to be the best handling of the three original heavy-weight lifting bodies (M2-F2/F3, HL-10, X-24A). The HL-10 was flown 37 times during the lifting body research program and logged the highest altitude and fastest speed in the Lifting Body program. On Feb. 18, 1970, Air Force

Cultivation of Anaplasma ovis in the HL-60 human promyelocytic leukemia cell line.

PubMed

Wei, Ran; Liu, Hong-Bo; Jongejan, Frans; Jiang, Bao-Gui; Chang, Qiao-Cheng; Fu, Xue; Jiang, Jia-Fu; Jia, Na; Cao, Wu-Chun

2017-09-20

The tick-borne bacterium Anaplasma ovis is a widely distributed pathogen affecting sheep, goats and wild ruminants. Here, the HL-60 human promyelocytic leukemia cell line was used to isolate A. ovis from PCR-positive sheep and goats in Heilongjiang Province, China. Two weeks after inoculation, morulae were observed in cytoplasmic vacuoles in four different HL-60 cultures. Confocal microscopy using a Cy3-labeled A. ovis-specific probe confirmed that the HL-60 cells were infected with A. ovis. Cells from the 6th HL-60 subculture displayed positive fluorescence when incubated with A. ovis antiserum in the indirect fluorescent antibody assay. PCR amplification and sequencing of 16S rRNA, groEL, gltA, msp2 and msp4 Anaplasma genes revealed that the four A. ovis culture isolates were identical. Phylogenetic analysis showed that the sequences clustered with other A. ovis strains but could clearly be distinguished from other Anaplasma species. When the 18th subculture of infected HL-60 cells was examined by electron microscopy, lysosomes were often observed near the vacuoles. After the 24th subculture, Giemsa staining and PCR indicated that the HL-60 cells were negative for A. ovis. Although A. ovis can infect HL-60 cells for only four months, the ability of the organism to infect and multiply in HL-60 cells provides a tool to study intra-erythrocytic Anaplasma and host cell interactions.

X-15A-2 and HL-10 parked on NASA ramp

NASA Technical Reports Server (NTRS)

1966-01-01

The HL-10 is shown next to the X-15A-2 in 1966. Both aircraft later went on to set records. On October 3, 1967, the X-15A-2 reached a speed of Mach 6.7, which was the highest speed achieved by a piloted aircraft until the Space Shuttles far exceeded that speed in 1981 and afterwards. The HL-10 later became the fastest piloted lifting body when it flew at a speed of Mach 1.86 on February 18, 1970. The HL-10 was one of five heavyweight lifting-body designs flown at NASA's Flight Research Center (FRC--later Dryden Flight Research Center), Edwards, California, from July 1966 to November 1975 to study and validate the concept of safely maneuvering and landing a low lift-over-drag vehicle designed for reentry from space. Northrop Corporation built the HL-10 and M2-F2, the first two of the fleet of 'heavy' lifting bodies flown by the NASA Flight Research Center. The contract for construction of the HL-10 and the M2-F2 was $1.8 million. 'HL' stands for horizontal landing, and '10' refers to the tenth design studied by engineers at NASA's Langley Research Center, Hampton, Va. After delivery to NASA in January 1966, the HL-10 made its first flight on Dec. 22, 1966, with research pilot Bruce Peterson in the cockpit. Although an XLR-11 rocket engine was installed in the vehicle, the first 11 drop flights from the B-52 launch aircraft were powerless glide flights to assess handling qualities, stability, and control. In the end, the HL-10 was judged to be the best handling of the three original heavy-weight lifting bodies (M2-F2/F3, HL-10, X-24A). The HL-10 was flown 37 times during the lifting body research program and logged the highest altitude and fastest speed in the Lifting Body program. On Feb. 18, 1970, Air Force test pilot Peter Hoag piloted the HL-10 to Mach 1.86 (1,228 mph). Nine days later, NASA pilot Bill Dana flew the vehicle to 90,030 feet, which became the highest altitude reached in the program. Some new and different lessons were learned through the successful

Selections from 2016: Gaps in HL Tau's Protoplanetary Disk

NASA Astrophysics Data System (ADS)

Kohler, Susanna

2016-12-01

Editors note:In these last two weeks of 2016, well be looking at a few selections that we havent yet discussed on AAS Nova from among the most-downloaded paperspublished in AAS journals this year. The usual posting schedule will resume after the AAS winter meeting.Gas Gaps in the Protoplanetary Disk Around the Young Protostar HL TauPublished March 2016The dust (left) and gas (right) emission from HL Tau show that the gaps in its disk match up. [Yen et al. 2016]Main takeaway:At the end of last year, the Atacama Large Millimeter/Submillimeter Array released some of its first data including a spectacular observation of a dusty protoplanetary disk around the young star HL Tau. In this follow-up study, a team led by Hsi-Wei Yen (Academia Sinica Institute of Astronomy and Astrophysics, Taiwan) analyzed the ALMA data and confirmed the presence of two gaps in the gas of HL Taus disk, at radii of 28 and 69 AU.Why its interesting:The original ALMA image of HL Taus disk suggests the presence of gaps in disk, but scientists werent sure if they were caused by effects like gravitational instabilities or dust clumping, or if the gaps were created by the presence of young planets. Yen and collaborators showed that gaps in the disks gas line up with gaps in its dust, supporting the model in which these gaps have been carved out by newly formed planets.Added intrigue:The evidence for planets in this disk came as a bit of a surprise, since it was originally believed that it takes tens of millions of years to form planets from the dust of protoplanetary disks but HL Tau is only a million years old. These observations therefore suggest that planets start to form much earlier than we thought.CitationHsi-Wei Yen et al 2016 ApJL 820 L25. doi:10.3847/2041-8205/820/2/L25

Integration of IEEE 1451 and HL7 exchanging information for patients' sensor data.

PubMed

Kim, Wooshik; Lim, Suyoung; Ahn, Jinsoo; Nah, Jiyoung; Kim, Namhyun

2010-12-01

HL7 (Health Level 7) is a standard developed for exchanging incompatible healthcare information generated from programs or devices among heterogenous medical information systems. At present, HL7 is growing as a global standard. However, the HL7 standard does not support effective methods for treating data from various medical sensors, especially from mobile sensors. As ubiquitous systems are growing, HL7 must communicate with various medical transducers. In the area of sensor fields, IEEE 1451 is a group of standards for controlling transducers and for communicating data from/to various transducers. In this paper, we present the possibility of interoperability between the two standards, i.e., HL7 and IEEE 1451. After we present a method to integrate them and show the preliminary results of this approach.

Expression of the transforming growth factor alpha protooncogene in differentiating human promyelocytic leukemia (HL-60) cells.

PubMed

Walz, T M; Malm, C; Wasteson, A

1993-01-01

The process of myeloid differentiation in human promyelocytic leukemia cells (HL-60) is accompanied by the coordinate expression of numerous protooncogenes. To investigate the expression of transforming growth factor alpha (TGF-alpha) in myeloid differentiation, HL-60 cells were induced to differentiate into granulocytes with 1.25% dimethyl sulfoxide, 0.2 microM all-trans retinoic acid, or 500 microM N6,O2-dibutyryladenosine-3'5'-cyclic monophosphate or differentiated along the monocyte/macrophage pathway with 0.1 microM phorbol-12-myristate-13-acetate. Using Northern blot analyses, TGF-alpha transcripts were detected within 24 h of treatment in cells differentiating toward granulocytes; maximal levels of gene expression were reached after 3 days or later and remained essentially constant throughout the observation period. These cells released TGF-alpha protein, as demonstrated by analysis of the incubation medium. In contrast, no TGF-alpha RNA or protein was detectable in HL-60 cell cultures when induced with phorbol-12-myristate-13-acetate. Epidermal growth factor receptor transcripts could not be detected either in undifferentiated or in differentiated HL-60 cells; therefore it appears as if an autocrine loop involving TGF-alpha in HL-60 cells is unlikely. In conclusion, the results demonstrate, for the first time, the expression of TGF-alpha in human granulocyte precursor cells. Our findings may indicate novel regulatory pathways in hematopoiesis.

Iron Overload and Apoptosis of HL-1 Cardiomyocytes: Effects of Calcium Channel Blockade

PubMed Central

Chen, Mei-pian; Cabantchik, Z. Ioav; Chan, Shing; Chan, Godfrey Chi-fung; Cheung, Yiu-fai

2014-01-01

Background Iron overload cardiomyopathy that prevails in some forms of hemosiderosis is caused by excessive deposition of iron into the heart tissue and ensuing damage caused by a raise in labile cell iron. The underlying mechanisms of iron uptake into cardiomyocytes in iron overload condition are still under investigation. Both L-type calcium channels (LTCC) and T-type calcium channels (TTCC) have been proposed to be the main portals of non-transferrinic iron into heart cells, but controversies remain. Here, we investigated the roles of LTCC and TTCC as mediators of cardiac iron overload and cellular damage by using specific Calcium channel blockers as potential suppressors of labile Fe(II) and Fe(III) ingress in cultured cardiomyocytes and ensuing apoptosis. Methods Fe(II) and Fe(III) uptake was assessed by exposing HL-1 cardiomyocytes to iron sources and quantitative real-time fluorescence imaging of cytosolic labile iron with the fluorescent iron sensor calcein while iron-induced apoptosis was quantitatively measured by flow cytometry analysis with Annexin V. The role of calcium channels as routes of iron uptake was assessed by cell pretreatment with specific blockers of LTCC and TTCC. Results Iron entered HL-1 cardiomyocytes in a time- and dose-dependent manner and induced cardiac apoptosis via mitochondria-mediated caspase-3 dependent pathways. Blockade of LTCC but not of TTCC demonstrably inhibited the uptake of ferric but not of ferrous iron. However, neither channel blocker conferred cardiomyocytes with protection from iron-induced apoptosis. Conclusion Our study implicates LTCC as major mediators of Fe(III) uptake into cardiomyocytes exposed to ferric salts but not necessarily as contributors to ensuing apoptosis. Thus, to the extent that apoptosis can be considered a biological indicator of damage, the etiopathology of cardiosiderotic damage that accompanies some forms of hemosiderosis would seem to be unrelated to LTCC or TTCC, but rather to other

Advanced Tokamak Stability Theory

NASA Astrophysics Data System (ADS)

Zheng, Linjin

2015-03-01

The intention of this book is to introduce advanced tokamak stability theory. We start with the derivation of the Grad-Shafranov equation and the construction of various toroidal flux coordinates. An analytical tokamak equilibrium theory is presented to demonstrate the Shafranov shift and how the toroidal hoop force can be balanced by the application of a vertical magnetic field in tokamaks. In addition to advanced theories, this book also discusses the intuitive physics pictures for various experimentally observed phenomena.

Extracting 3D Information from 1D and 2D Diagnostic Systems on the DIII-D Tokamak

NASA Astrophysics Data System (ADS)

Brookman, Michael

2017-10-01

The interpretation of tokamak data often hinges on assumptions of axisymetry and flux surface equilibria, neglecting 3D effects. This work discusses examples on the DIII-D tokamak where this assumption is an insufficient approximation, and explores the diagnostic information available to resolve 3D effects while preserving 1D profiles. Methods for extracting 3D data from the electron cyclotron emission radiometers, density profile reflectometer, and Thomson scattering system are discussed. Coordinating diagnostics around the tokamak shows the significance of 3D features, such as sawteeth[1] and resonant magnetic perturbations. A consequence of imposed 3D perturbations is a shift in major radius of measured profiles between diagnostics at different toroidal locations. Integrating different diagnostics requires a database containing information about their toroidal, poloidal, and radial locations. Through the data analysis framework OMFIT, it is possible to measure the magnitude of the apparent shifts from 3D effects and enforce consistency between diagnostics. Using the existing 1D and 2D diagnostic systems on DIII-D, this process allows the effects of the 3D perturbations on 1D profiles to be addressed. Supported by US DOE contracts DE-FC02-04ER54698, DE-FG03-97ER54415.

Energetic electrons, hard x-ray emission and MHD activity studies in the IR-T1 tokamak.

PubMed

Agah, K Mikaili; Ghoranneviss, M; Elahi, A Salar

2015-01-01

Determinations of plasma parameters as well as the Magnetohydrodynamics (MHD) activity, energetic electrons energy and energy confinement time are essential for future fusion reactors experiments and optimized operation. Also some of the plasma information can be deduced from these parameters, such as plasma equilibrium, stability, and MHD instabilities. In this contribution we investigated the relation between energetic electrons, hard x-ray emission and MHD activity in the IR-T1 Tokamak. For this purpose we used the magnetic diagnostics and a hard x-ray spectroscopy in IR-T1 tokamak. A hard x-ray emission is produced by collision of the runaway electrons with the plasma particles or limiters. The mean energy was calculated from the slope of the energy spectrum of hard x-ray photons.

Nanostructured cavity devices for extracellular stimulation of HL-1 cells

NASA Astrophysics Data System (ADS)

Czeschik, Anna; Rinklin, Philipp; Derra, Ulrike; Ullmann, Sabrina; Holik, Peter; Steltenkamp, Siegfried; Offenhäusser, Andreas; Wolfrum, Bernhard

2015-05-01

Microelectrode arrays (MEAs) are state-of-the-art devices for extracellular recording and stimulation on biological tissue. Furthermore, they are a relevant tool for the development of biomedical applications like retina, cochlear and motor prostheses, cardiac pacemakers and drug screening. Hence, research on functional cell-sensor interfaces, as well as the development of new surface structures and modifications for improved electrode characteristics, is a vivid and well established field. However, combining single-cell resolution with sufficient signal coupling remains challenging due to poor cell-electrode sealing. Furthermore, electrodes with diameters below 20 µm often suffer from a high electrical impedance affecting the noise during voltage recordings. In this study, we report on a nanocavity sensor array for voltage-controlled stimulation and extracellular action potential recordings on cellular networks. Nanocavity devices combine the advantages of low-impedance electrodes with small cell-chip interfaces, preserving a high spatial resolution for recording and stimulation. A reservoir between opening aperture and electrode is provided, allowing the cell to access the structure for a tight cell-sensor sealing. We present the well-controlled fabrication process and the effect of cavity formation and electrode patterning on the sensor's impedance. Further, we demonstrate reliable voltage-controlled stimulation using nanostructured cavity devices by capturing the pacemaker of an HL-1 cell network.Microelectrode arrays (MEAs) are state-of-the-art devices for extracellular recording and stimulation on biological tissue. Furthermore, they are a relevant tool for the development of biomedical applications like retina, cochlear and motor prostheses, cardiac pacemakers and drug screening. Hence, research on functional cell-sensor interfaces, as well as the development of new surface structures and modifications for improved electrode characteristics, is a vivid and

Secure HL7 transactions using Internet mail (Internet draft).

PubMed

Schadow, Gunther; Tucker, Mark; Rishel, Wes

2002-01-01

The document describes the applicability of the Internet standardisation efforts on secure electronic data interchange (EDI) transactions for Health Level-7 (HL7), an EDI standard for Healthcare used world-wide. The document heavily relies on the work in progress by the IETF EDIINT working group. It is in most parts a restatement of the EDIINTs requirements document and application statement 1 (AS#1) tailored to the needs of the HL7 audience. The authors tried to make the document as self consistent as possible. The goal is to give to the reader who is not a security or Internet standards expert enough foundational and detail information to enable him to build communication software that complies to the Internet standards. Even though the authors rely on and promote the respective Internet standards and drafts, they did not withstand from commenting on and criticising the work where they see upcoming problems in use with HL7 or other EDI protocols that have not been in the initial focus of the EDIINT working group. The authors make suggestions to add parameters to the specification of the MIME type for EDI messages in RFC 1767 in order to enhance functionality. The authors give use cases for a larger subset of disposition types and modifiers of message disposition notifications. One key issue where the document goes beyond the current EDIINT drafts is the concept of non-repudiation of commitment to an EDI transaction. Secure EDI transactions should be regarded as "distributed contracts," i.e. not only the sending and receiving of single messages should be non-refutable but also the connection between messages interchanges. In anticipation of this requirement HL7 usually requires a response message to be sent to acknowledge every transaction. The authors therefore have the requirement to securely couple an EDI response message to its request message. Given the current shape of RFC 1767 this is generally possible only if a response message is coupled with an MDN

An enhanced tokamak startup model

NASA Astrophysics Data System (ADS)

Goswami, Rajiv; Artaud, Jean-François

2017-01-01

The startup of tokamaks has been examined in the past in varying degree of detail. This phase typically involves the burnthrough of impurities and the subsequent rampup of plasma current. A zero-dimensional (0D) model is most widely used where the time evolution of volume averaged quantities determines the detailed balance between the input and loss of particle and power. But, being a 0D setup, these studies do not take into consideration the co-evolution of plasma size and shape, and instead assume an unchanging minor and major radius. However, it is known that the plasma position and its minor radius can change appreciably as the plasma evolves in time to fill in the entire available volume. In this paper, an enhanced model for the tokamak startup is introduced, which for the first time takes into account the evolution of plasma geometry during this brief but highly dynamic period by including realistic one-dimensional (1D) effects within the broad 0D framework. In addition the effect of runaway electrons (REs) has also been incorporated. The paper demonstrates that the inclusion of plasma cross section evolution in conjunction with REs plays an important role in the formation and development of tokamak startup. The model is benchmarked against experimental results from ADITYA tokamak.

HL-A and silicosis.

PubMed

Gualde, N; De Leobardy, J; Serizay, B; Malinvaud, O

1977-08-01

The search for a link between HL-A antigens and silicosis among 75 patients revealed no significant association. However, we did note a decrease in the frequency of B7; This change could be linked to a greater sensitivity to silicosis among persons who carry this antigen. There was also an increase of B8 among those silicotic patients with tuberculosis, although only a small number of patients were affected.

Real-time simulation model of the HL-20 lifting body

NASA Technical Reports Server (NTRS)

Jackson, E. Bruce; Cruz, Christopher I.; Ragsdale, W. A.

1992-01-01

A proposed manned spacecraft design, designated the HL-20, has been under investigation at Langley Research Center. Included in that investigation are flight control design and flying qualities studies utilizing a man-in-the-loop real-time simulator. This report documents the current real-time simulation model of the HL-20 lifting body vehicle, known as version 2.0, presently in use at NASA Langley Research Center. Included are data on vehicle aerodynamics, inertias, geometries, guidance and control laws, and cockpit displays and controllers. In addition, trim case and dynamic check case data is provided. The intent of this document is to provide the reader with sufficient information to develop and validate an equivalent simulation of the HL-20 for use in real-time or analytical studies.

The HL-LHC Accelerator Physics Challenges

NASA Astrophysics Data System (ADS)

Fartoukh, S.; Zimmermann, F.

The conceptual baseline of the HL-LHC project is reviewed, putting into perspective the main beam physics challenges of this new collider in comparison with the existing LHC, and the series of solutions and possible mitigation measures presently envisaged.

Direct Interaction of CaVβ with Actin Up-regulates L-type Calcium Currents in HL-1 Cardiomyocytes*

PubMed Central

Stölting, Gabriel; de Oliveira, Regina Campos; Guzman, Raul E.; Miranda-Laferte, Erick; Conrad, Rachel; Jordan, Nadine; Schmidt, Silke; Hendriks, Johnny; Gensch, Thomas; Hidalgo, Patricia

2015-01-01

Expression of the β-subunit (CaVβ) is required for normal function of cardiac L-type calcium channels, and its up-regulation is associated with heart failure. CaVβ binds to the α1 pore-forming subunit of L-type channels and augments calcium current density by facilitating channel opening and increasing the number of channels in the plasma membrane, by a poorly understood mechanism. Actin, a key component of the intracellular trafficking machinery, interacts with Src homology 3 domains in different proteins. Although CaVβ encompasses a highly conserved Src homology 3 domain, association with actin has not yet been explored. Here, using co-sedimentation assays and FRET experiments, we uncover a direct interaction between CaVβ and actin filaments. Consistently, single-molecule localization analysis reveals streaklike structures composed by CaVβ2 that distribute over several micrometers along actin filaments in HL-1 cardiomyocytes. Overexpression of CaVβ2-N3 in HL-1 cells induces an increase in L-type current without altering voltage-dependent activation, thus reflecting an increased number of channels in the plasma membrane. CaVβ mediated L-type up-regulation, and CaVβ-actin association is prevented by disruption of the actin cytoskeleton with cytochalasin D. Our study reveals for the first time an interacting partner of CaVβ that is directly involved in vesicular trafficking. We propose a model in which CaVβ promotes anterograde trafficking of the L-type channels by anchoring them to actin filaments in their itinerary to the plasma membrane. PMID:25533460

Nanostructured cavity devices for extracellular stimulation of HL-1 cells.

PubMed

Czeschik, Anna; Rinklin, Philipp; Derra, Ulrike; Ullmann, Sabrina; Holik, Peter; Steltenkamp, Siegfried; Offenhäusser, Andreas; Wolfrum, Bernhard

2015-01-01

Microelectrode arrays (MEAs) are state-of-the-art devices for extracellular recording and stimulation on biological tissue. Furthermore, they are a relevant tool for the development of biomedical applications like retina, cochlear and motor prostheses, cardiac pacemakers and drug screening. Hence, research on functional cell-sensor interfaces, as well as the development of new surface structures and modifications for improved electrode characteristics, is a vivid and well established field. However, combining single-cell resolution with sufficient signal coupling remains challenging due to poor cell-electrode sealing. Furthermore, electrodes with diameters below 20 µm often suffer from a high electrical impedance affecting the noise during voltage recordings. In this study, we report on a nanocavity sensor array for voltage-controlled stimulation and extracellular action potential recordings on cellular networks. Nanocavity devices combine the advantages of low-impedance electrodes with small cell-chip interfaces, preserving a high spatial resolution for recording and stimulation. A reservoir between opening aperture and electrode is provided, allowing the cell to access the structure for a tight cell-sensor sealing. We present the well-controlled fabrication process and the effect of cavity formation and electrode patterning on the sensor's impedance. Further, we demonstrate reliable voltage-controlled stimulation using nanostructured cavity devices by capturing the pacemaker of an HL-1 cell network.

Li Experiments at the Tokamak T-11M Toward PFC Concept of Steady State Tokamak-Reactor

NASA Astrophysics Data System (ADS)

Mirnov, S. V.

2009-11-01

As practical method of using a liquid lithium as a renewable plasma-facing component (PCF) for steady state tokamak-reactor the concept of lithium emitter-collector is considered [1]. It is based on lithium filled capillary porous system proposed by V.A. Evtikhin et al. (1996). The lithium circulation process consists of four steps: (1) Li emission from the PFC emitter into the plasma; (2) plasma boundary cooling by non-coronal Li radiation; (3) Li ion capture by the collector (before they are lost to the tokamak chamber wall); (4) Li return from the collector to the emitter. T-11M tokamak experiments have used three local rail limiters made from lithium, molybdenum and graphite as lithium collectors. The lithium behavior was studied by analysis of the witness samples, and by a mobile graphite probe. The key findings are: (1) lithium collection on the ion side of the lithium limiter is 2-3 times larger than on the electron side; (2) total efficiency of Li collection integrated over all three rail limiters can reach 50-70% of the lithium emission during the discharge pulse, while the theoretical limit is about 90%. [1] S.V. Mirnov, J. Nucl. Mat., 390-391, 876 (2009).

Initial hydrogenation during catabolism of picric acid by Rhodococcus erythropolis HL 24-2.

PubMed Central

Lenke, H; Knackmuss, H J

1992-01-01

Rhodococcus erythropolis HL 24-2, which was originally isolated as a 2,4-dinitrophenol-degrading bacterium, could also utilize picric acid as a nitrogen source after spontaneous mutation. During growth, the mutant HL PM-1 transiently accumulated an orange-red metabolite, which was identified as a hydride-Meisenheimer complex of picric acid. This complex was formed as the initial metabolite and further converted with concomitant liberation of nitrite. 2,4,6-Trinitrocyclohexanone was identified as a dead-end metabolite of the degradation of picric acid, indicating the addition of two hydride ions to picric acid. PMID:1444408

Effect of citrus flavonoids on HL-60 cell differentiation.

PubMed

Kawaii, S; Tomono, Y; Katase, E; Ogawa, K; Yano, M

1999-01-01

Twenty-seven Citrus flavonoids were examined for their activity of induction of terminal differentiation of human promyelocytic leukemia cells (HL-60) by nitro blue tetrazolium (NBT) reducing, nonspecific esterase, specific esterase, and phagocytic activities. 10 flavonoids were judged to be active (percentage of NBT reducing cells was more than 40% at a concentration of 40 microM), and the rank order of potency was natsudaidain, luteolin, tangeretin, quercetin, apigenin, 3, 3, '4, '5, 6, 7, 8-heptamethoxyflavone, nobiletin, acacetin, eriodictyol, and taxifolin. These flavonoids exerted their activity in a dose-dependent manner. HL-60 cells treated with these flavonoids differentiated into mature monocyte/macrophage. The structure-activity relationship established from comparison between flavones and flavanones revealed that ortho-catechol moiety in ring B and C2-C3 double bond had an important role for induction of differentiation of HL-60. In polymethoxylated flavones, hydroxyl group at C3 and methoxyl group at C8 enhanced the differentiation-inducing activity.

Observation of finite-. beta. MHD phenomena in tokamaks

DOE Office of Scientific and Technical Information (OSTI.GOV)

McGuire, K.M.

1984-09-01

Stable high-beta plasmas are required for the tokamak to attain an economical fusion reactor. Recently, intense neutral beam heating experiments in tokamaks have shown new effects on plasma stability and confinement associated with high beta plasmas. The observed spectrum of MHD fluctuations at high beta is clearly dominated by the n = 1 mode when the q = 1 surface is in the plasma. The m/n = 1/1 mode drives other n = 1 modes through toroidal coupling and n > 1 modes through nonlinear coupling. On PDX, with near perpendicular injection, a resonant interaction between the n = 1more » internal kink and the trapped fast ions results in loss of beam particles and heating power. Key parameters in the theory are the value of q/sub 0/ and the injection angle. High frequency broadband magnetic fluctuations have been observed on ISX-B and D-III and a correlation with the deterioration of plasma confinement was reported. During enhanced confinement (H-mode) discharges in divertor plasmas, two new edge instabilities were observed, both localized radially near the separatrix. By assembling results from the different tokamak experiments, it is found that the simple theoretical ideal MHD beta limit has not been exceeded. Whether this represents an ultimate tokamak limit or if beta optimized configurations (Dee- or bean-shaped plasmas) can exceed this limit and perhaps enter a second regime of stability remains to be clarified.« less

OHD/HL/HSMB - Hydrologic Science & Modeling Branch

Science.gov Websites

apply these sciences to application software and data products developed within the HL and as a hydrologic services program. HSMB applies its scientific expertise to training material developed

Direct interaction of CaVβ with actin up-regulates L-type calcium currents in HL-1 cardiomyocytes.

PubMed

Stölting, Gabriel; de Oliveira, Regina Campos; Guzman, Raul E; Miranda-Laferte, Erick; Conrad, Rachel; Jordan, Nadine; Schmidt, Silke; Hendriks, Johnny; Gensch, Thomas; Hidalgo, Patricia

2015-02-20

Expression of the β-subunit (CaVβ) is required for normal function of cardiac L-type calcium channels, and its up-regulation is associated with heart failure. CaVβ binds to the α1 pore-forming subunit of L-type channels and augments calcium current density by facilitating channel opening and increasing the number of channels in the plasma membrane, by a poorly understood mechanism. Actin, a key component of the intracellular trafficking machinery, interacts with Src homology 3 domains in different proteins. Although CaVβ encompasses a highly conserved Src homology 3 domain, association with actin has not yet been explored. Here, using co-sedimentation assays and FRET experiments, we uncover a direct interaction between CaVβ and actin filaments. Consistently, single-molecule localization analysis reveals streaklike structures composed by CaVβ2 that distribute over several micrometers along actin filaments in HL-1 cardiomyocytes. Overexpression of CaVβ2-N3 in HL-1 cells induces an increase in L-type current without altering voltage-dependent activation, thus reflecting an increased number of channels in the plasma membrane. CaVβ mediated L-type up-regulation, and CaVβ-actin association is prevented by disruption of the actin cytoskeleton with cytochalasin D. Our study reveals for the first time an interacting partner of CaVβ that is directly involved in vesicular trafficking. We propose a model in which CaVβ promotes anterograde trafficking of the L-type channels by anchoring them to actin filaments in their itinerary to the plasma membrane. © 2015 by The American Society for Biochemistry and Molecular Biology, Inc.

Is HL Tauri and FU Orionis system in quiescence?

NASA Technical Reports Server (NTRS)

Lin, D. N. C.; Hayashi, M.; Bell, K. R.; Ohashi, N.

1994-01-01

A recent Nobeyama map of HL Tau reveals that gas is infalling in a flattened region approximately 1400 AU around the central star. The apparent motion of the gas provides the necessary condition for the formation of a Keplerian disk with a radius comparable to the size of the primordial solar nebula. The inferred mass infall rate onto the disk is approximately equal to 5 x 10(exp -6) solar mass/yr, which greatly exceeds the maximum estimate of the accretion rate onto the central star (approximately 7 x 10(exp -7) solar mass/yr). Consequently, mass must currently be accumulating in the disk. The estimated age and disk mass of HL Tau suggest that the accumulated matter has been flushed repeatedly on a timescale less than 10(exp 4) yr. Based on the similarites between their evolution patterns, we propose that HL Tau is an FU Orionis system in quiescence. In addition to HL Tau, 14 out of 86 pre-main-sequence stars in the Taurus-Auriga dark clouds have infrared luminosities much greater than their otherwise normal extinction-corrected stellar luminosities. These sources also tend to have flat spectra which may be due to the reprocessing of radiation by dusty, flattened, collapsing envelopes with infall rates a few 10(exp -6) solar mass/yr. Such rates are much larger than estimated central accretion rates for these systems, which suggests that mass must also be accumulating in these disks. If these sources are FU Orionis stars in quiescence, similar to HL Tau, their age and relative abundance imply that the FU Orionis phase occurs over a timescale of approixmately 10(exp 5) yr, and the quiescent phase between each outburst lasts approximately 10(exp 3) =10(exp 4) yr. These inferred properties are compatible with the scenario that FU Orionis outbursts are regulated by a thermal instability in the inner region of the disk.

Ethyl acetate fraction of Garcina epunctata induces apoptosis in human promyelocytic cells (HL-60) through the ROS generation and G0/G1 cell cycle arrest: a bioassay-guided approach.

PubMed

Constant Anatole, Pieme; Guru, Santoh Kumar; Bathelemy, Ngamegni; Jeanne, Ngogang; Bhushan, Shashi; Murayama, Tetsuya; Saxena, Ajit Kumar

2013-11-01

Number of deaths due to cancer diseases is increasing in the world. There is an urgent need to develop alternative therapeutic measures against the disease. Our study reports the cytotoxicity activity of Garcina epunctata (gutifferae) in human promyelocytic leukemia cells (HL-60) and prostate cancer cells (PC-3) was evaluated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT). Changes in mitochondrial membrane potential (MMP), reactive oxygen species (ROS) and morphological changes associated with apoptosis were examined by flow cytometry and Hoescht staining respectively. The results of in vitro antiproliferative screening of fractions and extract from G. epunctata indicated that three fractions inhibited the viability of PC-3 cells with IC₅₀ varied from 50 to 88 μ/ml while two fractions inhibited the proliferation of HL-60 cells with IC₅₀ range between 47.5 and 12 μg/ml. Among the entire fraction tested, Hex-EtOAc (75:25) showed cytotoxic effects on the two cell lines and EtOAc fraction was most active only HL-60 cells (12 μg/ml). Treatment of HL-60 cells with G. epunctata (20, 50, 100 μg/ml) for 24 h led to a significant dose-dependent increase in the percentage of cells in sub-G1 phase by analysis of the content of DNA in cells, and a number of apoptotic bodies containing nuclear fragments were observed in cells treated with 100 μg/ml. The EtOAc fraction of G. epunctata treatment significantly arrested HL-60 cells at the G0/G1 phase (p<0.05) and ROS was significantly elevated as well as the loss of membrane mitochondrial potential in a concentration dependant manner. The results demonstrated that the EtOAc fraction of G. epunctata inhibited the proliferation of HL-60 cells, leading to cell cycle arrest and programmed cell death, which was confirmed to occur through the mitochondrial pathway. Copyright © 2013 Elsevier B.V. All rights reserved.

HL-LHC and HE-LHC Upgrade Plans and Opportunities for US Participation

NASA Astrophysics Data System (ADS)

Apollinari, Giorgio

2017-01-01

The US HEP community has identified the exploitation of physics opportunities at the High Luminosity-LHC (HL-LHC) as the highest near-term priority. Thanks to multi-year R&D programs, US National Laboratories and Universities have taken the leadership in the development of technical solutions to increase the LHC luminosity, enabling the HL-LHC Project and uniquely positioning this country to make critical contributions to the LHC luminosity upgrade. This talk will describe the shaping of the US Program to contribute in the next decade to HL-LHC through newly developed technologies such as Nb3Sn focusing magnets or superconducting crab cavities. The experience gained through the execution of the HL-LHC Project in the US will constitute a pool of knowledge and capabilities allowing further developments in the future. Opportunities for US participations in proposed hadron colliders, such as a possible High Energy-LHC (HE-LHC), will be described as well.

The Design of Dual-Emissive Composite Material [Zn2(HL)3]+@MOF-5 as Self-Calibrating Luminescent Sensors of Al3+ Ions and Monoethanolamine.

PubMed

Wu, Meng-Meng; Wang, Jiao-Yang; Sun, Rui; Zhao, Cui; Zhao, Jiong-Peng; Che, Guang-Bo; Liu, Fu-Chen

2017-08-21

Introducing another chromophore into a luminescent MOF is a potential way to assembling novel dual-emissive luminescent materials. Putting the chromophore, for which luminescence can be enhanced by Zn 2+ ion, into MOF-5 by the "bottle around ship" strategy is a simple but efficient synthesis method to realize such dual-emissive materials. According to this strategy, a novel dual-emissive luminescent composite material [Zn 2 (HL) 3 ] + @MOF-5 was constructed by loading the [La 3 (HL) 2 L 2 (NO 3 ) 3 H 2 O] (1) (H 2 L = 7,7'-(ethane-1,1'-diyl)8-hydro-quinoline) into MOF-5, in which the [Zn 2 (HL) 3 ] + anions were transformed from 1 with the existence of Zn 2+ . The dual-emissive composite materials show excellent luminescence with two emissions of MOF-5 at 410 nm and [Zn 2 (HL) 3 ] + at 524 nm. Furthermore, by combining characteristics of MOF-5 and the guest chromophore, the composite material is highly selectively sensitive toward Al 3+ and monoethanolamine, which makes [Zn 2 (HL) 3 ] + @MOF-5 a potential self-calibrated fluorescence sensor.

The Ling 6(HL) test: typical pediatric performance data and clinical use evaluation.

PubMed

Glista, Danielle; Scollie, Susan; Moodie, Sheila; Easwar, Vijayalakshmi

2014-01-01

The Ling 6(HL) test offers a calibrated version of naturally produced speech sounds in dB HL for evaluation of detection thresholds. Aided performance has been previously characterized in adults. The purpose of this work was to evaluate and refine the Ling 6(HL) test for use in pediatric hearing aid outcome measurement. This work is presented across two studies incorporating an integrated knowledge translation approach in the characterization of normative and typical performance, and in the evaluation of clinical feasibility, utility, acceptability, and implementation. A total of 57 children, 28 normally hearing and 29 with binaural sensorineural hearing loss, were included in Study 1. Children wore their own hearing aids fitted using Desired Sensation Level v5.0. Nine clinicians from The Network of Pediatric Audiologists participated in Study 2. A CD-based test format was used in the collection of unaided and aided detection thresholds in laboratory and clinical settings; thresholds were measured clinically as part of routine clinical care. Confidence intervals were derived to characterize normal performance and typical aided performance according to hearing loss severity. Unaided-aided performance was analyzed using a repeated-measures analysis of variance. The audiologists completed an online questionnaire evaluating the quality, feasibility/executability, utility/comparative value/relative advantage, acceptability/applicability, and interpretability, in addition to recommendation and general comments sections. Ling 6(HL) thresholds were reliably measured with children 3-18 yr old. Normative and typical performance ranges were translated into a scoring tool for use in pediatric outcome measurement. In general, questionnaire respondents generally agreed that the Ling 6(HL) test was a high-quality outcome evaluation tool that can be implemented successfully in clinical settings. By actively collaborating with pediatric audiologists and using an integrated

Lipase-catalyzed preparation of optically active 1'-acetoxychavicol acetates and their structure-activity relationships in apoptotic activity against human leukemia HL-60 cells.

PubMed

Azuma, Hideki; Miyasaka, Keita; Yokotani, Tsuyoshi; Tachibana, Taro; Kojima-Yuasa, Akiko; Matsui-Yuasa, Isao; Ogino, Kenji

2006-03-15

Structure-activity relationships of 1'-acetoxychavicol acetate (ACA) for apoptotic activity against human leukemia HL-60 cells were investigated using optically active ACA and various racemic ACA analogues. Natural-type (or with different acyl group) ACA showed a high apoptotic activity, but the ortho or meta isomers, 4-deacetoxy analogue, and the 2'-3' dehydrogenated derivative had no effect, or a weak activity. Optically active (R)- and (S)-ACA were prepared by a lipase-catalyzed esterification. Using a mixture of vinyl acetate-tetrahydrofuran (1:1 v/v) as a solvent at refluxing temperature, optically pure (R)- and (S)-ACA were obtained (99.7% ee and 99.1% ee, respectively). The apoptosis-inducing effects of both enantiomers were compared by means of an MTT assay and the detection of typical apoptotic phenomena (DNA fragmentation, caspase-3 activation, and PARP cleavage) and these two activities were almost equal. These results indicate that the essential moieties of ACA for apoptotic activity against HL-60 cells are both the presence of a 4-acetoxyl group and an unsaturated double bond between C-2' and C-3', and that the configuration at the 1'-position is unrelated to activity.

Polyreactivity of natural antibodies: exchange by HL-fragments.

PubMed

Sedykh, M A; Buneva, V N; Nevinsky, G A

2013-12-01

The polyreactivity of binding (formation of antibody (AB) complexes not only with specific but also with foreign antigens) is a widespread phenomenon that in some cases can be caused by a conformational lability of the antigen-binding sites of antibodies (which increases upon treatment with various destabilizing agents) and leads to AB binding with very different antigens. Some ABs exist as dimers of the initial ABs and their idiotypes (or anti-idiotypes) capable of producing intramolecular cyclic complexes with features of polyreactants. Another mechanism of binding polyreactivity is an exchange in blood by halves of IgG4 molecules (HL-fragments) against various antigens. Also, for the first time catalytic polyfunctionality of human milk ABs has been detected, which is caused by an exchange by HL-fragments between molecules of λ- and κ-IgG (IgG1-IgG4) and also by λ- and κ-sIgA against different antigens with formation of very different chimeric antibodies. This review considers all possible pathways of formation of polyspecific immunoglobulins and their biological functions described in the literature, as well as mechanisms of binding polyreactivity and catalytic polyfunctionality of natural antibodies.

Impedance of an intense plasma-cathode electron source for tokamak startup

NASA Astrophysics Data System (ADS)

Hinson, E. T.; Barr, J. L.; Bongard, M. W.; Burke, M. G.; Fonck, R. J.; Perry, J. M.

2016-05-01

An impedance model is formulated and tested for the ˜1 kV , 1 kA/cm2 , arc-plasma cathode electron source used for local helicity injection tokamak startup. A double layer sheath is established between the high-density arc plasma ( narc≈1021 m-3 ) within the electron source, and the less dense external tokamak edge plasma ( nedge≈1018 m-3 ) into which current is injected at the applied injector voltage, Vinj . Experiments on the Pegasus spherical tokamak show that the injected current, Iinj , increases with Vinj according to the standard double layer scaling Iinj˜Vinj3 /2 at low current and transitions to Iinj˜Vinj1 /2 at high currents. In this high current regime, sheath expansion and/or space charge neutralization impose limits on the beam density nb˜Iinj/Vinj1 /2 . For low tokamak edge density nedge and high Iinj , the inferred beam density nb is consistent with the requirement nb≤nedge imposed by space-charge neutralization of the beam in the tokamak edge plasma. At sufficient edge density, nb˜narc is observed, consistent with a limit to nb imposed by expansion of the double layer sheath. These results suggest that narc is a viable control actuator for the source impedance.

Mechanism and characteristics of stimuli-dependent ROS generation in undifferentiated HL-60 cells.

PubMed

Muranaka, Shikibu; Fujita, Hirofumi; Fujiwara, Takuzo; Ogino, Tetsuya; Sato, Eisuke F; Akiyama, Jitsuo; Imada, Isuke; Inoue, Masayasu; Utsumi, Kozo

2005-01-01

It has been widely believed that undifferentiated human promyelocytic leukemia cells (HL-60) have no ability to generate reactive oxygen species (ROS) responding to stimuli. We report here that undifferentiated HL-60 cells possess NADPH oxidase and that generation of superoxide can be measured using a highly sensitive chemiluminescence dye, L-012. Five subunits of NADPH oxidase, namely, gp91(phox), p22(phox), p67(phox), p47(phox), and Rac 2, were detected in undifferentiated HL-60 cells by immunoblotting analysis. The contents of these NADPH oxidase components in the cells were increased with the differentiation induced by phorbol myristate acetate (PMA), except for p22(phox). Messenger RNAs of these subunits were also detected by the RT-PCR method, and their expressions increased except that of p22(phox) with the differentiation induced by PMA. Kinetic analysis using L-012 revealed that HL-60 cells generated substantial amounts of ROS by various stimulants, including formylmethionyl-leucyl-phenylalanine, PMA, myristic acid, and a Ca2+ ionophore, A23187. Both diphenyleneiodonium (an inhibitor of FAD-dependent oxidase) and apocynin (a specific inhibitor of NADPH oxidase) suppressed this stimuli-dependent ROS generation. Genistein, staurosporine, uric acid, and sodium azide inhibited the ROS generation in undifferentiated HL-60 cells in a similar way to that in undifferentiated neutrophils. These results suggested that the mechanism of ROS generation in undifferentiated HL-60 cells is the same as that in primed neutrophils.

[Studies on red orpiment induction of NB4 and HL-60 cell apoptosis].

PubMed

Bai, Y; Huang, S

1998-09-01

To study the possible mechanism of red orpiment, which is main component of composite indigo naturalis tablets, in the treatment of acute promyelocytic leukemia(APL). The effect of red orpiment on induction of APL cell line NB4 and HL-60 apoptosis were studied by cell morphology, DNA gel electrophoresis and flow cytometry assay. Red orpiment induced NB4 and HL-60 cell apoptosis. When treated with different concentration of red orpiment(25-200 micrograms/ml) for 16 hours, both NB4 and HL-60 cells showed typical apoptosis features. If decreased the concentration of red orpiment to 12.5 micrograms/ml, the NB4 cell still showed apoptosis features while the HL-60 cell did not when cultured for 72 hours. Arsenic disulfide(As2S2) had the same effect as red orpiment did under the same experiment condition. It is the main component, As2S2 of the red orpiment that can induces NB4 and HL-60 cell apoptosis. and the red orpiment is responsible for the high CR rate of APL induced by the composite indigo naturalis tablets.

Nuclear CD38 in retinoic acid-induced HL-60 cells

DOE Office of Scientific and Technical Information (OSTI.GOV)

Yalcintepe, Leman; Albeniz, Isil; Adin-Cinar, Suzan

2005-02-01

The cell surface antigen, CD38, is a 45-kDa transmembrane protein which is predominantly expressed on hematopoietic cells during differentiation. As a bifunctional ectoenzyme, it catalyzes the synthesis of cyclic ADP-ribose (cADPR) from NAD{sup +} and hydrolysis of either NAD{sup +} or cADPR to ADP-ribose. All-trans-retinoic acid (RA) is a potent and specific inducer of CD38 in myeloid cells. In this report, we demonstrate that the nuclei of RA-treated human HL-60 myeloblastic cells reveal enzymatic activities inherent to CD38. Thus, GDP-ribosyl cyclase and NAD{sup +} glycohydrolase activities in the nuclear fraction increased very significantly in response to incubation with RA. Withmore » Western blotting, we detected in the nuclear protein fraction from RA-treated cells a {approx}43-kDa protein band which was reactive with the CD38-specific monoclonal antibody OKT10. The expression of CD38 in HL-60 nuclei was also shown with FACScan analysis. RA treatment gave rise to an increase in in vitro ADP ribosylation of the {approx}43-kDa nuclear protein. Moreover, nuclei isolated from RA-treated HL-60 cells revealed calcium release in response to cADPR, whereas a similar response was not observed in control nuclei. These results suggest that CD38 is expressed in HL-60 cell nuclei during RA-induced differentiation.« less

Induction of non-apoptotic cell death by morphinone in human promyelocytic leukemia HL-60 cells.

PubMed

Takeuchi, Risa; Hoshijima, Hiroshi; Nagasaka, Hiroshi; Chowdhury, Shahead Ali; Kikuchi, Hirotaka; Kanda, Yumiko; Kunii, Shiro; Kawase, Masami; Sakagami, Hiroshi

2006-01-01

As previously suggested, codeinone (oxidation product of codeine) induces non-apoptotic cell death, characterized by marginal caspase activation and the lack of DNA fragmentation in HL-60 human promyelocytic leukemia cells, which was inhibited by N-acetyl-L-cysteine. Whether, morphinone, an oxidative metabolite of morphine, also induced a similar type of cell death in HL-60 cells was investigated. Morphinone showed slightly higher cytotoxic activity against human tumor cell lines (oral squamous cell carcinoma HSC-2, HSC-3, HSC-4, NA, Ca9-22, promyelocytic leukemia HL-60, cervical carcinoma HeLa) than against normal oral human cells (gingival fibroblast HGF, pulp cells HPC, periodontal ligament fibroblast HPLF). Morphinone also induced an almost undetectable level of internucleosomal DNA fragmentation in the HL-60 cells. Morphinone did not activate caspase-8 or -9 in these cells. Morphinone dose-dependently activated caspase-3 in both HL-60 and HSC-2 cell lines, but to a much lesser extent than actinomycin D. Electron microscopy demonstrated that morphinone induced mitochondrial shrinkage, vacuolization and production of autophagosome and the loss of cell surface microvilli, without destruction of cell surface and nuclear membranes in the HL-60 cells. The autophagy inhibitor 3-methyladenine (0.3-10 mM) slightly inhibited the morphinone-induced cytotoxicity, when corrected for its own cytotoxicity. These data suggest that morphinone induces non-apoptotic cell death in HL-60 cells.

Current drive by spheromak injection into a tokamak

DOE Office of Scientific and Technical Information (OSTI.GOV)

Brown, M.R.; Bellan, P.M.

1990-04-30

We report the first observation of current drive by injection of a spheromak plasma into a tokamak (Caltech ENCORE small reasearch tokamak) due to the process of helicity injection. After an abrupt 30% increase, the tokamak current decays by a factor of 3 due to plasma cooling caused by the merging of the relatively cold spheromak with the tokamak. The tokamak density profile peaks sharply due to the injected spheromak plasma ({ital {bar n}}{sub 3} increases by a factor of 6) then becomes hollow, suggestive of an interchange instability.

BCL-x{sub L}/MCL-1 inhibition and RARγ antagonism work cooperatively in human HL60 leukemia cells

DOE Office of Scientific and Technical Information (OSTI.GOV)

Perri, Mariarita; Yap, Jeremy L.; Yu, Jianshi

2014-10-01

The acute promyelocytic leukemia (APL) subtype of acute myeloid leukemia (AML) is characterized by chromosomal translocations that result in fusion proteins, including the promyelocytic leukemia–retinoic acid receptor, alpha fusion protein (PML–RARα). All-trans retinoic acid (atRA) treatment is the standard drug treatment for APL yielding cure rates >80% by activating transcription and proteasomal degradation of retinoic acid receptor, alpha (RARα). Whereas combination therapy with As{sub 2}O{sub 3} has increased survival further, patients that experience relapse and are refractory to atRA and/or As{sub 2}O{sub 3} is a clinically significant problem. BCL-2 family proteins regulate apoptosis and over-expression of anti-apoptotic B-cell leukemia/lymphoma 2more » (BCL-2) family proteins has been associated with chemotherapeutic resistance in APL including impairment of the ability of atRA to induce growth arrest and differentiation. Here we investigated the novel BH3 domain mimetic, JY-1-106, which antagonizes the anti-apoptotic BCL-2 family members B-cell lymphoma-extra large (BCL-x{sub L}) and myeloid cell leukemia-1 (MCL-1) alone and in combination with retinoids including atRA, AM580 (RARα agonist), and SR11253 (RARγ antagonist). JY-1-106 reduced cell viability in HL-60 cells alone and in combination with retinoids. The combination of JY-1-106 and SR11253 had the greatest impact on cell viability by stimulating apoptosis. These studies indicate that dual BCL-x{sub L}/MCL-1 inhibitors and retinoids could work cooperatively in leukemia treatment. - Highlights: • Novel Bcl-x{sub L}/Mcl-1 inhibitor JY-1-106 reduces HL60 cell viability. • JY-1-106 is investigated in combination with retinoic acid, AM580, and SR11253. • AM580 is an RARα agonist; SR11253 is an RARγ antagonist. • Combined use of JY-1-106/SR11253 exhibited the greatest cell viability reduction. • JY-1-106 alone or in combination with retinoids induces apoptosis.« less

[Establishment of Z-HL16C cell line.].

PubMed

Chen, J P; Li, J; Zhao, S L; Tian, J Y; Ye, F

2006-09-01

To establish and study the nature and the application of Z-HL16C cell line. The cell line was continuously passed, frozen stored and recovered. Its application was expanded and the cell type was identified. The cell line had an epithelial-cell-like shape, the size appeared uniform, the cell boundary was distinct. It has been continuously passed, frozen stored and recovered for ten years. Its recovery rate was about 90%. It has been proved to be sensitive to the tested viruses which were enteroviruses (Polio, Cox, Echo), influenza viruses, parainfluenzaviruses, adenoviruses, measles virus. This cell line has been identified as a cancerization cell. The cell line Z-HL16C has been stably established, it has a broad spectrum in sensitivity for culturing viruses.

Gamma ray imager on the DIII-D tokamak

DOE Office of Scientific and Technical Information (OSTI.GOV)

Pace, D. C., E-mail: pacedc@fusion.gat.com; Taussig, D.; Eidietis, N. W.

2016-04-15

A gamma ray camera is built for the DIII-D tokamak [J. Luxon, Nucl. Fusion 42, 614 (2002)] that provides spatial localization and energy resolution of gamma flux by combining a lead pinhole camera with custom-built detectors and optimized viewing geometry. This diagnostic system is installed on the outer midplane of the tokamak such that its 123 collimated sightlines extend across the tokamak radius while also covering most of the vertical extent of the plasma volume. A set of 30 bismuth germanate detectors can be secured in any of the available sightlines, allowing for customizable coverage in experiments with runaway electronsmore » in the energy range of 1–60 MeV. Commissioning of the gamma ray imager includes the quantification of electromagnetic noise sources in the tokamak machine hall and a measurement of the energy spectrum of background gamma radiation. First measurements of gamma rays coming from the plasma provide a suitable testbed for implementing pulse height analysis that provides the energy of detected gamma photons.« less

Gamma ray imager on the DIII-D tokamak

DOE PAGES

Pace, D. C.; Cooper, C. M.; Taussig, D.; ...

2016-04-13

A gamma ray camera is built for the DIII-D tokamak [J. Luxon, Nucl. Fusion 42, 614 (2002)] that provides spatial localization and energy resolution of gamma flux by combining a lead pinhole camera with custom-built detectors and optimized viewing geometry. This diagnostic system is installed on the outer midplane of the tokamak such that its 123 collimated sightlines extend across the tokamak radius while also covering most of the vertical extent of the plasma volume. A set of 30 bismuth germanate detectors can be secured in any of the available sightlines, allowing for customizable coverage in experiments with runaway electronsmore » in the energy range of 1- 60 MeV. Commissioning of the gamma ray imager includes the quantification of electromagnetic noise sources in the tokamak machine hall and a measurement of the energy spectrum of background gamma radiation. In conclusion, first measurements of gamma rays coming from the plasma provide a suitable testbed for implementing pulse height analysis that provides the energy of detected gamma photons.« less

Quantitative study of sniffer leak rate and pressure drop leak rate of liquid nitrogen panels of SST-1 tokamak

NASA Astrophysics Data System (ADS)

Pathan, F. S.; Khan, Z.; Semwal, P.; Raval, D. C.; Joshi, K. S.; Thankey, P. L.; Dhanani, K. R.

2008-05-01

Steady State Super-conducting (SST-1) Tokamak is in commissioning stage at Institute for Plasma Research. Vacuum chamber of SST-1 Tokamak consists of 1) Vacuum vessel, an ultra high vacuum (UHV) chamber, 2) Cryostat, a high vacuum (HV) chamber. Cryostat encloses the liquid helium cooled super-conducting magnets (TF and PF), which require the thermal radiation protection against room temperature. Liquid nitrogen (LN2) cooled panels are used to provide thermal shield around super-conducting magnets. During operation, LN2 panels will be under pressurized condition and its surrounding (cryostat) will be at high vacuum. Hence, LN2 panels must have very low leak rate. This paper describes an experiment to study the behaviour of the leaks in LN2 panels during sniffer test and pressure drop test using helium gas.

Modulation of transferrin receptor mRNA by transferrin-gallium in human myeloid HL60 and lymphoid CCRF-CEM leukaemic cells.

PubMed Central

Ul-Haq, R; Chitambar, C R

1993-01-01

Gallium binds to the iron transport protein transferrin (Tf), is incorporated into cells through transferrin receptors (TfR) and inhibits iron-dependent DNA synthesis. Since cellular TfR expression is tightly regulated by the availability of iron, we investigated the effects of transferrin-gallium (Tf-Ga) on TfR mRNA levels in myeloid HL60 and lymphoid CCRF-CEM cells. In HL60 cells, Tf-Ga increased TfR mRNA levels in a dose-dependent fashion. This increase in TfR mRNA was blocked by Tf-Fe and by cycloheximide. Analysis of the rate of mRNA decay in the presence of actinomycin D revealed that the half-life of TfR mRNA was increased in HL60 cells incubated with Tf-Ga. The rate of transcription of TfR mRNA was not increased by Tf-Ga. In contrast with HL60 cells, CCRF-CEM cells displayed a decrease in the level of TfR mRNA after incubation with Tf-Ga. Tf-Ga inhibited iron uptake in both HL60 and CCRF-CEM cells but increased the level of TfR mRNA only in HL60 cells, suggesting that the Tf-Ga induction of TfR mRNA was not solely due to inhibition of cellular iron uptake. At growth-inhibitory concentrations, Tf-Ga increased the TfR mRNA level in HL60 cells but decreased it in CCRF-CEM cells. Our studies suggest that in HL60 cells, gallium regulates TfR expression at the post-transcriptional level by mechanisms which require de novo protein synthesis and involve interaction with iron. The divergent effects of Tf-Ga on TfR mRNA in myeloid HL60 and lymphoid CCRF-CEM cells suggest that differences exist in the regulation of TfR expression between these two cell types. Images Figure 1 Figure 2 Figure 3 Figure 4 Figure 5 Figure 6 PMID:8379943

Upper limits to the detection of ammonia from protoplanetary disks around HL Tauri and L1551-IRS 5

NASA Technical Reports Server (NTRS)

Gomez, Jose F.; Torrelles, Jose M.; Ho, Paul T. P.; Rodriguez, Luis F.; Canto, Jorge

1993-01-01

We present NH3(1, 1) and (2, 2) observations of the young stellar sources HL Tau and L1551-IRS 5 using the VLA in its B-configuration, which provides an angular resolution of about 0.4 arcsec (about 50 AU at 140 pc) at 1.3 cm wavelength. Our goal was to detect and resolve circumstellar molecular disks with radius of the order of 100 AU around these two sources. No ammonia emission was detected toward either of them. The 3-sigma levels were 2.7 mJy/beam and 3.9 mJy/beam for HL Tau and L1551-IRS 5, respectively, with a velocity resolution of about 5 km/s. With this nondetection, we estimate upper limits to the mass of the proposed protoplanetary molecular disks (within a radius of 10 AU from the central stars) on the order of 0.02/(X(NH3)/10 exp -8) solar mass for HL Tau and 0.1/(X(NH3)/10 exp -8) solar mass for L1551-IRS 5.

c-fms mRNA is regulated posttranscriptionally by 1,25(OH)2D3 in HL-60 cells.

PubMed

Biskobing, D M; Fan, D; Rubin, J

1997-09-01

Macrophage colony-stimulating factor (MCSF) is required for normal osteoclast and macrophage development. The receptor for MCSF (c-fms) is expressed on the pluripotent precursor and mature osteoclasts and macrophages. We have previously shown in myelomonocytic HL-60 cells that phorbol myristate acetate (PMA) upregulates c-fms mRNA expression. This induction of c-fms is inhibited by 1,25(OH)2D3. The major regulatory control of c-fms mRNA levels by PMA has been identified as posttranscriptional. However, a role of transcript elongation in controlling levels of c-fms mRNA has also been suggested. To better understand the 1,25(OH)2D3 regulation of c-fms mRNA expression we studied nuclear run on, mRNA stability, and transcript elongation in HL-60 cells treated with 10 ng/ml phorbol myristate acetate, 10 nM 1,25(OH)2D3 alone or combined. We demonstrated by nuclear run on that c-fms was constitutively transcribed in 1,25(OH)2D3 as well as control and PMA-treated cells. Transcript elongation was evaluated by RT-PCR for exon 2 or exon 3. Both exons were minimally expressed in control and 1,25(OH)2D3-treated cells, and increased in PMA-treated cells; this increased expression was inhibited by the addition of 1,25(OH)2D3. These results fail to show differential transcript elongation. Measurement of mRNA stability demonstrated decreased mRNA half-life to 5 hours in cells treated with PMA and 1,25(OH)2D3 compared with a half-life of 8 hours in cells treated with PMA alone. Our findings demonstrate that c-fms is regulated by 1,25(OH)2D3 at the posttranscriptional level by changes in mRNA stability. This gives the cell the ability to respond to local signals with rapid changes in c-fms levels altering the ability of the cell to respond to MCSF.

Undifferentiated HL-60 cells internalize an antitumor alkyl ether phospholipid more rapidly than resistant K562 cells.

PubMed

Tsutsumi, T; Tokumura, A; Kitazawa, S

1998-02-05

In this study, we confirmed a previous finding that 1-O-octadecyl-2-O-methyl-rac-glycero-3-phosphocholine (methyl-PAF) expresses higher antineoplastic activity against the promyelocytic leukemia cell line HL-60, than against the erythroleukemic cell line K562, and intended to clarify the reason for this. Using an albumin back-exchange method, we measured the rates of binding and internalization of [3H]methyl-PAF by HL-60 and K562 cells. We found that methyl-PAF associated very rapidly and to similar extents with the two types of cells at low concentrations of extracellular bovine serum albumin, but that when bound to the cell surface, it was internalized into HL-60 cells faster than into K562 cells. The internalization of methyl-PAF by HL-60 cells was concentration-independent, intracellular ATP-independent and susceptible to thiol group-modifying reagents and cytochalasin B. Thus the inward transbilayer movement of methyl-PAF seems to occur by cytochalasin B-sensitive protein-mediated mechanism based on passive diffusion not requiring energy, in which SH-groups of protein play a critical role. We also found that the internalization of 1-hexadecanoyl-2-(4,4-difluoro-5,7- dimethyl-4-bora-3a, 4a-diaza-s-indacene-3-pentanoyl)-sn-glycero-3-phosphocholine (Bodipy-C5-PC), whose structure resembles that of methyl-PAF, into HL-60 cells was faster than that into K562 cells. Using a combination of an albumin back-exchange method and observation by confocal laser scanning microscopy, we next examined the intracellular distribution of this fluorescent phospholipid probe after its internalization. Intracellular membranes, especially those peripheral to nuclei, were fluorescence-labeled in both HL-60 and K562 cells, but fluorescence of the nuclear membranes was weak, suggesting that this probe seems mainly to accumulate in intracellular granules, and may interact directly with several key enzymes for phospholipid metabolism, leading to cell injury. Because the difference between

Ranatensin-HL: A Bombesin-Related Tridecapeptide from the Skin Secretion of the Broad-Folded Frog, Hylarana latouchii.

PubMed

Lin, Yan; Chen, Tianbao; Zhou, Mei; Wang, Lei; Su, Songkun; Shaw, Chris

2017-07-04

Bombesin-related peptides are a family of peptides whose prototype was discovered in amphibian skin and which exhibit a wide range of biological activities. Since the initial isolation of bombesin from Bombina bombina skin, diverse forms of bombesin-related peptides have been found in the skins across Anura. In this study, a novel bombesin-related peptide of the ranatensin subfamily, named ranatensin-HL, was structurally-characterised from the skin secretion of the broad-folded frog, Hylarana latouchii , through combination of molecular cloning and mass spectrometric methodologies. It is composed of 13 amino acid residues, pGlu-RAGNQWAIGHFM-NH₂, and resembles an N-terminally extended form of Xenopus neuromedin B. Ranatensin-HL and its C-terminal decapeptide (ranatensin-HL-10) were chemically synthesised and subjected to in vitro smooth muscle assays in which they were found to display moderate stimulatory effects on rat urinary bladder and uterus smooth muscles with EC 50 values in the range of 1-10 nM. The prepro-ranatensin-HL was highly homological to a bombesin-like peptide from Rana catesbeiana at both nucleotide and amino acid levels, which might provide a clue for the taxonomic classification of ranid frogs in the future.

Clinicopathologic consensus study of gray zone lymphoma with features intermediate between DLBCL and classical HL.

PubMed

Pilichowska, Monika; Pittaluga, Stefania; Ferry, Judith A; Hemminger, Jessica; Chang, Hong; Kanakry, Jennifer A; Sehn, Laurie H; Feldman, Tatyana; Abramson, Jeremy S; Kritharis, Athena; Hernandez-Ilizaliturri, Francisco J; Lossos, Izidore S; Press, Oliver W; Fenske, Timothy S; Friedberg, Jonathan W; Vose, Julie M; Blum, Kristie A; Jagadeesh, Deepa; Woda, Bruce; Gupta, Gaurav K; Gascoyne, Randy D; Jaffe, Elaine S; Evens, Andrew M

2017-12-12

Gray zone lymphoma (GZL) is described as sharing features with classical Hodgkin lymphoma (cHL) and diffuse large B-cell lymphoma (DLBCL). However, there remains complexity in establishing diagnosis, delineating prognosis, and determining optimum therapy. Sixty-eight cases diagnosed as GZL across 15 North American academic centers were evaluated by central pathology review to achieve consensus. Of these, only 26 (38%) were confirmed as GZL. Morphology was critical to GZL consensus diagnosis (eg, tumor cell richness); immunohistochemistry showed universal B-cell derivation, frequent CD30 expression, and rare Epstein-Barr virus (EBV) positivity (CD20 + , 83%; PAX5 + , 100%; BCL6 + , 20%; MUM1 + , 100%; CD30 + , 92%; EBV + , 4%). Forty-two cases were reclassified: nodular sclerosis (NS) cHL, n = 27 (including n = 10 NS grade 2); lymphocyte predominant HL, n = 4; DLBCL, n = 4; EBV + DLBCL, n = 3; primary mediastinal large BCL n = 2; lymphocyte-rich cHL and BCL-not otherwise specified, n = 1 each. GZL consensus-confirmed vs reclassified cases, respectively, more often had mediastinal disease (69% vs 41%; P = .038) and less likely more than 1 extranodal site (0% vs 25%; P = .019). With a 44-month median follow-up, 3-year progression-free survival (PFS) and overall survival for patients with confirmed GZL were 39% and 95%, respectively, vs 58% and 85%, respectively, for reclassified cases ( P = .19 and P = .15, respectively). Interestingly, NS grade 2 reclassified patients had similar PFS as GZL consensus-confirmed cases. For prognostication of GZL cases, hypoalbuminemia was a negative factor (3-year PFS, 12% vs 64%; P = .01), whereas frontline cyclophosphamide, doxorubicin, vincristine, and prednisone ± rituximab (CHOP±R) was associated with improved 3-year PFS (70% vs 20%; P = .03); both factors remained significant on multivariate analysis. Altogether, accurate diagnosis of GZL remains challenging, and improved therapeutic strategies are needed.

Clinicopathologic consensus study of gray zone lymphoma with features intermediate between DLBCL and classical HL

PubMed Central

Pilichowska, Monika; Pittaluga, Stefania; Ferry, Judith A.; Hemminger, Jessica; Chang, Hong; Kanakry, Jennifer A.; Sehn, Laurie H.; Feldman, Tatyana; Abramson, Jeremy S.; Kritharis, Athena; Hernandez-Ilizaliturri, Francisco J.; Lossos, Izidore S.; Press, Oliver W.; Fenske, Timothy S.; Friedberg, Jonathan W.; Vose, Julie M.; Blum, Kristie A.; Jagadeesh, Deepa; Woda, Bruce; Gupta, Gaurav K.; Gascoyne, Randy D.; Jaffe, Elaine S.

2017-01-01

Gray zone lymphoma (GZL) is described as sharing features with classical Hodgkin lymphoma (cHL) and diffuse large B-cell lymphoma (DLBCL). However, there remains complexity in establishing diagnosis, delineating prognosis, and determining optimum therapy. Sixty-eight cases diagnosed as GZL across 15 North American academic centers were evaluated by central pathology review to achieve consensus. Of these, only 26 (38%) were confirmed as GZL. Morphology was critical to GZL consensus diagnosis (eg, tumor cell richness); immunohistochemistry showed universal B-cell derivation, frequent CD30 expression, and rare Epstein-Barr virus (EBV) positivity (CD20+, 83%; PAX5+, 100%; BCL6+, 20%; MUM1+, 100%; CD30+, 92%; EBV+, 4%). Forty-two cases were reclassified: nodular sclerosis (NS) cHL, n = 27 (including n = 10 NS grade 2); lymphocyte predominant HL, n = 4; DLBCL, n = 4; EBV+ DLBCL, n = 3; primary mediastinal large BCL n = 2; lymphocyte-rich cHL and BCL–not otherwise specified, n = 1 each. GZL consensus-confirmed vs reclassified cases, respectively, more often had mediastinal disease (69% vs 41%; P = .038) and less likely more than 1 extranodal site (0% vs 25%; P = .019). With a 44-month median follow-up, 3-year progression-free survival (PFS) and overall survival for patients with confirmed GZL were 39% and 95%, respectively, vs 58% and 85%, respectively, for reclassified cases (P = .19 and P = .15, respectively). Interestingly, NS grade 2 reclassified patients had similar PFS as GZL consensus-confirmed cases. For prognostication of GZL cases, hypoalbuminemia was a negative factor (3-year PFS, 12% vs 64%; P = .01), whereas frontline cyclophosphamide, doxorubicin, vincristine, and prednisone ± rituximab (CHOP±R) was associated with improved 3-year PFS (70% vs 20%; P = .03); both factors remained significant on multivariate analysis. Altogether, accurate diagnosis of GZL remains challenging, and improved therapeutic strategies are needed. PMID:29296913

Impedance of an intense plasma-cathode electron source for tokamak startup

DOE PAGES

Hinson, Edward Thomas; Barr, Jayson L.; Bongard, Michael W.; ...

2016-05-31

In this study, an impedance model is formulated and tested for the ~1kV, ~1kA/cm 2, arc-plasma cathode electron source used for local helicity injection tokamak startup. A double layer sheath is established between the high-density arc plasma (n arc ≈ 10 21 m -3) within the electron source, and the less dense external tokamak edge plasma (n edge ≈ 10 18 m -3) into which current is injected at the applied injector voltage, V inj. Experiments on the Pegasus spherical tokamak show the injected current, I inj, increases with V inj according to the standard double layer scaling I injmore » ~ V inj 3/2 at low current and transitions to I inj ~ V inj 1/2 at high currents. In this high current regime, sheath expansion and/or space charge neutralization impose limits on the beam density n b ~ I inj/V inj 1/2. For low tokamak edge density n edge and high I inj, the inferred beam density n b is consistent with the requirement n b ≤ n edge imposed by space-charge neutralization of the beam in the tokamak edge plasma. At sufficient edge density, n b ~ n arc is observed, consistent with a limit to n b imposed by expansion of the double layer sheath. These results suggest that n arc is a viable control actuator for the source impedance.« less

Design for effective development and prototyping of the HL-20

NASA Astrophysics Data System (ADS)

Urie, David M.; Floreck, Paul A.; McMorris, John A.; Elvin, John D.

1993-10-01

A feasibility study of the HL-20 personnel launch system (PLS) concept was conducted by a team which focused on creating a PLS design approach and an accelerated development plan consistent with the historical 'Skunk Works' approach to rapid prototyping. Technical design, manufacturing, system testing, and operations and support elements of the predefined baseline concept were evaluated. An initial phase program, featuring a concurrent system test during design and development, leading to the orbital flight of an unmanned HL-20 prototype on a Titan III launch system, was prescribed. A second-phase development and manufacturing plan leading to system operational status was also formulated. Baseline design feature modifications were made when necessary, without compromise to performance, to satisfy the prototype development plan. Technical design details and off-the-shelf hardware candidates were also identified for several subsystems, including the launch-system interface adapter/emergency escape system. The technical feasibility of the system and applicability of the Skunk Works approach to development of the HL-20/PLS were verified.

Dynamical Stability of Imaged Planetary Systems in Formation: Application to HL Tau

NASA Astrophysics Data System (ADS)

Tamayo, D.; Triaud, A. H. M. J.; Menou, K.; Rein, H.

2015-06-01

A recent Atacama Large Millimeter/Submillimeter Array image revealed several concentric gaps in the protoplanetary disk surrounding the young star HL Tau. We consider the hypothesis that these gaps are carved by planets, and present a general framework for understanding the dynamical stability of such systems over typical disk lifetimes, providing estimates for the maximum planetary masses. We collect these easily evaluated constraints into a workflow that can help guide the design and interpretation of new observational campaigns and numerical simulations of gap opening in such systems. We argue that the locations of resonances should be significantly shifted in massive disks like HL Tau, and that theoretical uncertainties in the exact offset, together with observational errors, imply a large uncertainty in the dynamical state and stability in such disks. This presents an important barrier to using systems like HL Tau as a proxy for the initial conditions following planet formation. An important observational avenue to breaking this degeneracy is to search for eccentric gaps, which could implicate resonantly interacting planets. Unfortunately, massive disks like HL Tau should induce swift pericenter precession that would smear out any such eccentric features of planetary origin. This motivates pushing toward more typical, less massive disks. For a nominal non-resonant model of the HL Tau system with five planets, we find a maximum mass for the outer three bodies of approximately 2 Neptune masses. In a resonant configuration, these planets can reach at least the mass of Saturn. The inner two planets’ masses are unconstrained by dynamical stability arguments.

Oxidative Stress and Genotoxicity of Zinc Oxide Nanoparticles to Pseudomonas Species, Human Promyelocytic Leukemic (HL-60), and Blood Cells.

PubMed

Soni, Deepika; Gandhi, Deepa; Tarale, Prashant; Bafana, Amit; Pandey, R A; Sivanesan, Saravanadevi

2017-08-01

In the present study, toxicity of commercial zinc oxide nanoparticles (ZnO NPs) was studied on the bacterium Pseudomonas sp., human promyelocytic leukemia (HL-60) cells, and peripheral blood mononuclear cells (PBMC). The toxicity was assessed by measuring growth, cell viability, and protein expression in bacterial cell. The bacterial growth and viability decreased with increasing concentrations of ZnO NP. Three major proteins, ribosomal protein L1 and L9 along with alkyl hydroperoxides reductase, were upregulated by 1.5-, 1.7-, and 2.0-fold, respectively, after ZnO NP exposure. The results indicated oxidative stress as the leading cause of toxic effect in bacteria. In HL-60 cells, cytotoxic and genotoxic effects along with antioxidant enzyme activity and reactive oxygen species (ROS) generation were studied upon ZnO NP treatment. ZnO NP exhibited dose-dependent increase in cell death after 24-h exposure. The DNA-damaging potential of ZnO NP in HL-60 cells was maximum at 0.05 mg/L concentration. Comet assay showed 70-80% increase in tail DNA at 0.025 to 0.05 mg/L ZnO NP concentration. A significant increase of 1.6-, 1.4-, and 2.0-fold in ROS level was observed after 12 h. Genotoxic potential of ZnO NPs was also demonstrated in PBMC through DNA fragmentation. Thus, ZnO NP, besides being an essential element having antibacterial activity, also showed toxicity towards human cells (HL-60 and PBMC).

Architectural approaches for HL7-based health information systems implementation.

PubMed

López, D M; Blobel, B

2010-01-01

Information systems integration is hard, especially when semantic and business process interoperability requirements need to be met. To succeed, a unified methodology, approaching different aspects of systems architecture such as business, information, computational, engineering and technology viewpoints, has to be considered. The paper contributes with an analysis and demonstration on how the HL7 standard set can support health information systems integration. Based on the Health Information Systems Development Framework (HIS-DF), common architectural models for HIS integration are analyzed. The framework is a standard-based, consistent, comprehensive, customizable, scalable methodology that supports the design of semantically interoperable health information systems and components. Three main architectural models for system integration are analyzed: the point to point interface, the messages server and the mediator models. Point to point interface and messages server models are completely supported by traditional HL7 version 2 and version 3 messaging. The HL7 v3 standard specification, combined with service-oriented, model-driven approaches provided by HIS-DF, makes the mediator model possible. The different integration scenarios are illustrated by describing a proof-of-concept implementation of an integrated public health surveillance system based on Enterprise Java Beans technology. Selecting the appropriate integration architecture is a fundamental issue of any software development project. HIS-DF provides a unique methodological approach guiding the development of healthcare integration projects. The mediator model - offered by the HIS-DF and supported in HL7 v3 artifacts - is the more promising one promoting the development of open, reusable, flexible, semantically interoperable, platform-independent, service-oriented and standard-based health information systems.

HA117 endows HL60 cells with a stem-like signature by inhibiting the degradation of DNMT1 via its ability to down-regulate expression of the GGL domain of RGS6

PubMed Central

Li, Shuangshuang; Wu, Huan; Wang, Yi; Li, Xiaoqing; Guo, Yuxia; Liang, Shaoyan

2017-01-01

All-trans retinoic acid (ATRA) induces complete remission in almost all patients with acute promyelocytic leukemia (APL) via its ability to induce the in vivo differentiation of APL blasts. However, prolonged ATRA treatment can result in drug resistance. In previous studies, we generated a multi-drug-resistant HL60/ATRA cell line and found it to contain a new drug resistance-related gene segment, HA117. In this study, we demonstrate that ATRA induces multi-drug-resistant subpopulations of HL60 cells with a putative stem-like signature by up-regulating the expression of the new gene segment HA117. Western blot analysis and quantitative real-time PCR demonstrated that HA117 causes alternative splicing of regulator of G-protein signaling 6 (RGS6) and down-regulation of the expression of the GGL domain of RGS6, which plays an important role in DNA methyltransferase 1 (DNMT1) degradation. Moreover, DNMT1 expression was increased in multi-drug resistance HL60/ATRA cells. Knockdown of HA117 restored expression of the GGL domain and blocked DNMT1 expression. Moreover, resistant cells displayed a putative stem-like signature with increased expression of cancer steam cell markers CD133 and CD123. The stem cell marker, Nanog, was significantly up-regulated. In conclusion, our study shows that HA117 potentially promotes the stem-like signature of the HL60/ATRA cell line by inhibiting by the ubiquitination and degradation of DNMT1 and by down-regulating the expression of the GGL domain of RGS6. These results throw light on the cellular events associated with the ATRA-induced multi-drug resistance phenotype in acute leukemia. PMID:28665981

Analysis of leukotriene B4 metabolism in human promyelocytic HL-60 cells.

PubMed

Kasimir, S; Schönfeld, W; Hilger, R A; König, W

1991-10-01

We previously reported that human alveolar macrophages rapidly metabolize the chemotactic active lipid mediator leukotriene B4 (LTB4) into the dihydro-LTB4 by reduction of one of the conjugated double bonds. We herein report that human HL-60 cells (a myeloid precursor which can be differentiated into granulocyte- as well as monocyte-like cells by dimethyl sulphoxide or phorbol myristate acetate) express a highly active LTB4 reductase in the undifferentiated state. Differentiation by dimethyl sulphoxide (1.3%) along the granulocyte lineage, as confirmed by light microscopy, conversion of NitroBlue Tetrazolium into formazan, failed to induce a substantial capacity for omega-oxidation of LTB4; this reaction is exclusively found in mature granulocytes. Studies with the cell homogenate of undifferentiated HL-60 cells indicated that the activity of the enzyme depends on the presence of NADPH, Ca2+ and Mg2+, with a pH optimum of 7.5 at 37 degrees C. The enzyme was not released into the supernatant after stimulation of HL-60 cells with phorbol myristate acetate (100 ng) or Ca2+ ionophore (7.5 microM). Subcellular fractionation revealed evidence that the LTB4 reductase is located within the membrane fraction. Purification of the enzyme by gel filtration and gel electrophoresis suggests an apparent molecular mass of 40 kDa.

Introduction to the HL-LHC Project

NASA Astrophysics Data System (ADS)

Rossi, L.; Brüning, O.

The Large Hadron Collider (LHC) is one of largest scientific instruments ever built. It has been exploring the new energy frontier since 2010, gathering a global user community of 7,000 scientists. To extend its discovery potential, the LHC will need a major upgrade in the 2020s to increase its luminosity (rate of collisions) by a factor of five beyond its design value and the integrated luminosity by a factor of ten. As a highly complex and optimized machine, such an upgrade of the LHC must be carefully studied and requires about ten years to implement. The novel machine configuration, called High Luminosity LHC (HL-LHC), will rely on a number of key innovative technologies, representing exceptional technological challenges, such as cutting-edge 11-12 tesla superconducting magnets, very compact superconducting cavities for beam rotation with ultra-precise phase control, new technology for beam collimation and 300-meter-long high-power superconducting links with negligible energy dissipation. HL-LHC federates efforts and R&D of a large community in Europe, in the US and in Japan, which will facilitate the implementation of the construction phase as a global project.

The role of disk self-gravity on gap formation of the HL Tau proto-planetary disk

DOE PAGES

Li, Shengtai; Li, Hui

2016-05-31

Here, we use extensive global hydrodynamic disk gas+dust simulations with embedded planets to model the dust ring and gap structures in the HL Tau protoplanetary disk observed with the Atacama Large Millimeter/Submillimeter Array (ALMA). Since the HL Tau is a relatively massive disk, we find the disk self-gravity (DSG) plays an important role in the gap formation induced by the planets. Our simulation results demonstrate that DSG is necessary in explaining of the dust ring and gap in HL Tau disk. The comparison of simulation results shows that the dust rings and gap structures are more evident when the fullymore » 2D DSG (non-axisymmetric components are included) is used than if 1D axisymmetric DSG (only the axisymetric component is included) is used, or the disk self-gravity is not considered. We also find that the couple dust+gas+planet simulations are required because the gap and ring structure is different between dust and gas surface density.« less

Resistive edge mode instability in stellarator and tokamak geometries

NASA Astrophysics Data System (ADS)

Mahmood, M. Ansar; Rafiq, T.; Persson, M.; Weiland, J.

2008-09-01

Geometrical effects on linear stability of electrostatic resistive edge modes are investigated in the three-dimensional Wendelstein 7-X stellarator [G. Grieger et al., Plasma Physics and Controlled Nuclear Fusion Research 1990 (International Atomic Energy Agency, Vienna, 1991), Vol. 3, p. 525] and the International Thermonuclear Experimental Reactor [Progress in the ITER Physics Basis, Nucl. Fusion 7, S1, S285 (2007)]-like equilibria. An advanced fluid model is used for the ions together with the reduced Braghinskii equations for the electrons. Using the ballooning mode representation, the drift wave problem is set as an eigenvalue equation along a field line and is solved numerically using a standard shooting technique. A significantly larger magnetic shear and a less unfavorable normal curvature in the tokamak equilibrium are found to give a stronger finite-Larmor radius stabilization and a more narrow mode spectrum than in the stellarator. The effect of negative global magnetic shear in the tokamak is found to be stabilizing. The growth rate on a tokamak magnetic flux surface is found to be comparable to that on a stellarator surface with the same global magnetic shear but the eigenfunction in the tokamak is broader than in the stellarator due to the presence of large negative local magnetic shear (LMS) on the tokamak surface. A large absolute value of the LMS in a region of unfavorable normal curvature is found to be stabilizing in the stellarator, while in the tokamak case, negative LMS is found to be stabilizing and positive LMS destabilizing.

Antitumor effects with apoptotic death in human promyelocytic leukemia HL-60 cells and suppression of leukemia xenograft tumor growth by irinotecan HCl.

PubMed

Chen, Yung-Liang; Chueh, Fu-Shin; Yang, Jai-Sing; Hsueh, Shu-Ching; Lu, Chi-Cheng; Chiang, Jo-Hua; Lee, Ching-Sung; Lu, Hsu-Feng; Chung, Jing-Gung

2015-07-01

Irinotecan HCl (CPT-11) is an anticancer prodrug, but there is no available information addressing CPT-11-inhibited leukemia cells in in vitro and in vivo studies. Therefore, we investigated the cytotoxic effects of CPT-11 in promyelocytic leukemia HL-60 cells and in vivo and tumor growth in a leukemia xenograft model. Effects of CPT-11 on HL-60 cells were determined using flow cytometry, immunofluorescence staining, comet assay, real-time PCR, and Western blotting. CPT-11 demonstrated a dose- and time-dependent inhibition of cell growth, induction of apoptosis, and cell-cycle arrest at G0/G1 phase in HL-60 cells. CPT-11 promoted the release of AIF from mitochondria and its translocation to the nucleus. Bid, Bax, Apaf-1, caspase-9, AIF, Endo G, caspase-12, ATF-6b, Grp78, CDK2, Chk2, and cyclin D were all significantly upregulated and Bcl-2 was down-regulated by CPT-11 in HL-60 cells. Induction of cell-cycle arrest by CPT-11 was associated with changes in expression of key cell-cycle regulators such as CDK2, Chk2, and cyclin D in HL-60 cells. To test whether CPT-11 could augment antitumor activity in vivo, athymic BALB/c(nu/nu) nude mice were inoculated with HL-60 cells, followed by treatment with either CPT-11. The treatments significantly inhibited tumor growth and reduced tumor weight and volume in the HL-60 xenograft mice. The present study demonstrates the schedule-dependent antileukemia effect of CPT-11 using both in vitro and in vivo models. CPT-11 could potentially be a promising agent for the treatment of promyelocytic leukemia and requires further investigation. © 2014 Wiley Periodicals, Inc.

The Hellenic National Tsunami Warning Centre (HL-NTWC): Recent updates and future developments

NASA Astrophysics Data System (ADS)

Melis, Nikolaos S.; Charalampakis, Marinos

2014-05-01

The Hellenic NTWC (HL-NTWC) was established officially by Greek Law in September 2010. HL-NTWC is hosted at the National Observatory of Athens, Institute of Geodynamics (NOA-IG), which also operates a 24/7 earthquake monitoring service in Greece and coordinates the newly established Hellenic Unified National Seismic Network. NOA-IG and HL-NTWC Operational Centre is linked to the Civil Protection Operational Centre and serves as the official alerting agency to the General Secretariat for Civil Protection in Greece, regarding earthquake events and tsunami watch. Since August 2012, HL-NTWC acts as Candidate Tsunami Watch Provider (CTWP) under the UNESCO IOC - ICG NEAMTWS tsunami warning system (NEAM: North-Eastern Atlantic, the Mediterranean and connected seas) and offers its services to the NEAMTWS system. HL-NTWC has participated in all Communication Test Exercises (CTE) under NEAMTWS and also it has provided tsunami scenarios for extended system testing exercises such as NEAMWAVE12. Some of the recent developments at HL-NTWC in Greece include: deployment of new tide gauge stations for tsunami watch purposes, computation of tsunami scenarios and extending the database in use, improving alerting response times, earthquake magnitude estimation and testing newly established software modules for tsunami and earthquake alerting (i.e. Early-Est, SeisComP3 etc.) in Greece and the Eastern Mediterranean. Although funding today is limited, an advantage of the participation in important EC funded research projects, i.e. NERIES, NERA, TRANSFER, NEAMTIC and ASTARTE, demonstrates that collaboration of top class Research Institutions that care to produce important and useful results in the research front in Europe, can facilitate towards developing and operating top class Operational Centers, useful for Civil Protection purposes in regions in need. Last, it is demonstrated that HL-NTWC collaboration with important key role Research Centers in the Security and Safety issues (e

Steady State Advanced Tokamak (SSAT): The mission and the machine

NASA Astrophysics Data System (ADS)

Thomassen, K.; Goldston, R.; Nevins, B.; Neilson, H.; Shannon, T.; Montgomery, B.

1992-03-01

Extending the tokamak concept to the steady state regime and pursuing advances in tokamak physics are important and complementary steps for the magnetic fusion energy program. The required transition away from inductive current drive will provide exciting opportunities for advances in tokamak physics, as well as important impetus to drive advances in fusion technology. Recognizing this, the Fusion Policy Advisory Committee and the U.S. National Energy Strategy identified the development of steady state tokamak physics and technology, and improvements in the tokamak concept, as vital elements in the magnetic fusion energy development plan. Both called for the construction of a steady state tokamak facility to address these plan elements. Advances in physics that produce better confinement and higher pressure limits are required for a similar unit size reactor. Regimes with largely self-driven plasma current are required to permit a steady-state tokamak reactor with acceptable recirculating power. Reliable techniques of disruption control will be needed to achieve the availability goals of an economic reactor. Thus the central role of this new tokamak facility is to point the way to a more attractive demonstration reactor (DEMO) than the present data base would support. To meet the challenges, we propose a new 'Steady State Advanced Tokamak' (SSAT) facility that would develop and demonstrate optimized steady state tokamak operating mode. While other tokamaks in the world program employ superconducting toroidal field coils, SSAT would be the first major tokamak to operate with a fully superconducting coil set in the elongated, divertor geometry planned for ITER and DEMO.

The Spherical Tokamak MEDUSA for Mexico

NASA Astrophysics Data System (ADS)

Ribeiro, C.; Salvador, M.; Gonzalez, J.; Munoz, O.; Tapia, A.; Arredondo, V.; Chavez, R.; Nieto, A.; Gonzalez, J.; Garza, A.; Estrada, I.; Jasso, E.; Acosta, C.; Briones, C.; Cavazos, G.; Martinez, J.; Morones, J.; Almaguer, J.; Fonck, R.

2011-10-01

The former spherical tokamak MEDUSA (Madison EDUcation Small Aspect.ratio tokamak, R < 0.14m, a < 0.10m, BT < 0.5T, Ip < 40kA, 3ms pulse) is currently being recomissioned at the Universidad Autónoma de Nuevo León, Mexico, as part of an agreement between the Faculties of Mech.-Elect. Eng. and Phy. Sci.-Maths. The main objective for having MEDUSA is to train students in plasma physics & technical related issues, aiming a full design of a medium size device (e.g. Tokamak-T). Details of technical modifications and a preliminary scientific programme will be presented. MEDUSA-MX will also benefit any developments in the existing Mexican Fusion Network. Strong liaison within national and international plasma physics communities is expected. New activities on plasma & engineering modeling are expected to be developed in parallel by using the existing facilities such as a multi-platform computer (Silicon Graphics Altix XE250, 128G RAM, 3.7TB HD, 2.7GHz, quad-core processor), ancillary graph system (NVIDIA Quadro FE 2000/1GB GDDR-5 PCI X16 128, 3.2GHz), and COMSOL Multiphysics-Solid Works programs.

Role of gelsolin in the formation and organization of triton-soluble F-actin during myeloid differentiation of HL-60 cells.

PubMed

Watts, R G

1995-04-15

Structurally and functionally distinct F-actin pools coexist with globular (G)-actin in a variety of eukaryotic cells, including polymorphonuclear leukocytes (PMNs). In PMNs, a Triton-soluble F-actin pool (TSF) exists as short cytoplasmic filaments capped with gelsolin, while Triton-insoluble F-actin (TIF) is a three-dimensional meshwork of F-actin associated with actin-binding protein 280 (ABP-280), alpha-actinin, and tropomyosin. The unique association of gelsolin with the TSF suggests a role for gelsolin in creation or regulation of TSF. To evaluate gelsolin's role in TSF formation, the quantities of actin and gelsolin were determined by quantitative sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and immunoblots in uninduced HL-60 cells (U-HL-60) and in HL-60 cells induced to myeloid differentiation with 1.25% dimethyl sulfoxide for 4 to 5 days (I-HL-60). U-HL-60 cells contain 17.76 +/- 6.01 pmol actin per 10(6) cells (TIF, 5.3 +/- 1.5; TSF, 2.17 +/- 0.37; G, 10.3 +/- 5.7; n = 5) and 0.073 pmol gelsolin per 10(6) cells (TIF, 0; TSF, 0.002 +/- 0.005; G, 0.07 +/- 0.01; n = 3), representing molar actin to gelsolin (A:G) ratios of 1,085:1 for TSF and 147:1 for G. After myeloid differentiation, the actin content increases 1.80-fold (31.94 +/- 6.14 pmol/10(6) cells) equally in each actin pool (TIF, 9.36 +/- 2.35; TSF, 3.29 +/- 0.62; G, 19.29 +/- 4.83). Gelsolin increases 2.4-fold overall (0.178 +/- 0.02 pmol/10(6) cells) but 19-fold in TSF (0.038 +/- 0.009) and only 1.9-fold in G pool (0.139 +/- 0.006), resulting in A:G ratios of 87:1 in TSF and 139:1 in G. The findings of an increase in TSF gelsolin with decreased A:G ratios (1,085:1 v 87:1) with myeloid differentiation suggest shortening of TSF filaments, while the A:G ratios of unbound gelsolin are unchanged (147:1 v 139:1). Measurement of EGTA-resistant gelsolin/actin complexes in HL-60 cells shows that 95% to 100% of complexes exist in the TSF-actin pool only. These findings are consistent

Enhanced expression of extracellular calcium sensing receptor in monocyte-differentiated versus undifferentiated HL-60 cells: potential role in regulation of a nonselective cation channel

NASA Technical Reports Server (NTRS)

Yamaguchi, T.; Ye, C.; Chattopadhyay, N.; Sanders, J. L.; Vassilev, P. M.; Brown, E. M.; O'Malley, B. W. (Principal Investigator)

2000-01-01

Human promyelocytic leukemia cells (HL-60) have been used widely as a model for studying the differentiation of hematopoietic progenitor cells in vitro. After treatment with phorbol-12-myristate-13-acetate (PMA) or 1,25-dihydroxyvitamin D(3) [1,25(OH)(2)D(3)], HL-60 cells differentiate into cells with the phenotype of monocytes/macrophages. We previously showed that peripheral blood monocytes and the murine J774 monocytic cell line express the CaR, and myeloid progenitors in the bone marrow and myeloid cells in peripheral blood other than monocytes express lower levels of the CaR. Therefore, we investigated whether undifferentiated HL-60 cells express a functional G protein-coupled, extracellular calcium (Ca(2+)(o))-sensing receptor (CaR) and if the expression of the CaR increases as these cells differentiate along the monocytic lineage. The use of reverse transcription-polymerase chain reaction (RT-PCR) with CaR-specific primers, followed by sequencing of the amplified products, identified an authentic CaR transcript in undifferentiated HL-60 cells. Both immunocytochemistry and Western blot analysis using a CaR-specific antiserum detected low levels of CaR protein expression in undifferentiated HL-60 cells. The levels of CaR protein increased considerably following treatment of the cells with PMA (50 nM) or 1,25(OH)(2)D(3) (100 nM) for 5 days. Northern analysis using a CaR-specific riboprobe identified CaR transcripts in undifferentiated HL-60 cells, but CaR mRNA levels did not change appreciably after treatment with either agent, suggesting that upregulation of CaR protein occurs at a translational level. PMA-treated HL-60 cells expressed a nonselective cation channel (NCC), and the calcimimetic CaR activator, NPS R-467, but not its less active stereoisomer, NPS S-467, as well as the polycationic CaR agonist, neomycin, activated this NCC, demonstrating that the CaR expressed in these cells is functionally active. Therefore, HL-60 cells exhibit an increase in Ca

[Expression of ICAT and Wnt signaling-related proteins in the monocytic differentiation of HL-60 cells induced by a new steroidal drug NSC67657].

PubMed

Wang, J S; Wang, W J; Wang, T; Zhang, Y

2016-04-01

To investigate the expression of mRNA and proteins of β-catenin, TCF-4 (ICAT) and Wnt signaling pathway-related genes in the monocytic differentiation of acute myeloid leukemia HL-60 cells induced by a new steroidal drug NSC67657. Wright's staining and α-NBE staining were used to observe the differentiation of HL-60 cells after 5 days of 10 μmol/L NSC67657 treatment. Flow cytometry (FCM) was used to detect the differentiation and cell cycles. The expressions of mRNA and proteins of ICAT and Wnt signaling pathway-related factors, including β-catenin, TCF-4, c-myc, cyclin D1 and TCF-1 before and after differentiation, were detected by RT-PCR and Western blot. Morphological observation showed that NSC67657 induced monocytic differentiation of HL-60 cells. At 5 days after 10 μmol/L NSC67657 treatment, the number of CD14(+) HL-60 cells was (94.37±2.84)%, significantly higher than the (1.31±0.09)% in control group (P<0.01). The flow cytometry assay revealed that NSC67657 induced (76.46±2.83)% of G1/G0 phase arrest, significantly higher than that of (59.40±5.42)% in the control group (P<0.05), while the S phase cells were of (18.76±0.98)%, significantly lower than that of (34.38±2.61) % in the control group (P<0.05). The NSC67657 treatment also up-regulated the expression of ICAT mRNA and protein, and down-regulated the expression of β-catenin mRNA and protin (P<0.01 for all). However, the nuclear expression of β-catenin was down-regulated (P<0.01). The NSC67657 treatment induced nonsignificant alterations of TCF-4 mRNA, total protein and nuclear protein in the HL-60 cells (P>0.05 for all). The target genes of Wnt signaling pathway, including c-myc, cyclinD1 and TCF-1 mRNA and proteins in the HL-60 cells were significantly down-regulated after NSC67657 treatment (P<0.05). The new steroidal drug NSC67657 induces monocytic differentiation of HL-60 cells, and down-regulates the expression of β-catenin and target genes of Wnt signaling pathway. These results

Compact Torus Fueling of the STOR-M Tokamak

NASA Astrophysics Data System (ADS)

Xiao, C.; Hirose, A.; Zawalski, W.; White, D.; Raman, R.; Decoste, R.; Gregory, B. C.; Martin, F.

1996-11-01

Tangential injection of accelerated compact torus (CT) has been performed on the STOR-M tokamak (R/a=46/12 cm, B_t<1 T, I_p<= 50 kA, barn_e=(0.5 - 1)×10^13 cm-3) using the University of Saskatchewan Compact Torus Injector (USCTI). The CT parameters are: m~=1 μg, v=120 km/sec, B=0.1 T and n=(2 - 4)×10^15 cm-3. After CT injection, the electron density in tokamak doubles and the poloidal β-value increases. Indications of reduction in the loop voltage and H_α emission level have also been observed. Currently, following efforts are being made: (a) to coat chromium on the electrode surface, (b) to increase the on-line baking temperature, and (c) to reduce the neutral gas load which follows the CT plasma. In addition, numerical calculation of CT motion in a tokamak magnetic field has been carried out. For horizontal injection, the initial CT magnetic dipole direction should be aligned with the CT velocity for deeper penetration. In the case of vertical injection, the CT trajectory is independent of the initial magnetic dipole direction and central penetration is facilitated by off-axis injection.

The actions of mdivi-1, an inhibitor of mitochondrial fission, on rapidly activating delayed-rectifier K⁺ current and membrane potential in HL-1 murine atrial cardiomyocytes.

PubMed

So, Edmund Cheung; Hsing, Chung-Hsi; Liang, Chia-Hua; Wu, Sheng-Nan

2012-05-15

Mdivi-1 is an inhibitor of dynamin related protein 1- (drp1)-mediated mitochondrial fission. However, the mechanisms through which this compound interacts directly with ion currents in heart cells remain unknown. In this study, its effects on ion currents and membrane potential in murine HL-1 cardiomyocytes were investigated. In whole-cell recordings, the addition of mdivi-1 decreased the amplitude of tail current (I(tail)) for the rapidly activating delayed-rectifier K⁺ current (I(Kr)) in a concentration-dependent manner with an IC₅₀ value at 11.6 μM, a value that resembles the inhibition requirement for mitochondrial division. It shifted the activation curve of I(tail) to depolarized voltages with no change in the gating charge. However, mdivi-1 did not alter the rate of recovery from current inactivation. In cell-attached configuration, mdivi-1 inside the pipette suppressed the activity of acetylcholine-activated K⁺ channels without modifying the single-channel conductance. Mdivi-1 (30 μM) slightly depressed the peak amplitude of Na⁺ current with no change in the overall current-voltage relationship. Under current-clamp recordings, addition of mdivi-1 resulted in prolongation for the duration of action potentials (APs) and to increase the firing of spontaneous APs in HL-1 cells. Similarly, in pituitary GH₃ cells, mdivi-1 was effective in directly suppressing the amplitude of ether-à-go-go-related gene-mediated K⁺ current. Therefore, the lengthening of AP duration and increased firing of APs caused by mdivi-1 can be primarily explained by its inhibition of these K⁺ channels enriched in heart cells. The observed effects of mdivi-1 on ion currents were direct and not associated with its inhibition of mitochondrial division. Copyright © 2012 Elsevier B.V. All rights reserved.

Numerical analysis on the synergy between electron cyclotron current drive and lower hybrid current drive in tokamak plasmas

NASA Astrophysics Data System (ADS)

Chen, S. Y.; Hong, B. B.; Liu, Y.; Lu, W.; Huang, J.; Tang, C. J.; Ding, X. T.; Zhang, X. J.; Hu, Y. J.

2012-11-01

The synergy between electron cyclotron current drive (ECCD) and lower hybrid current drive (LHCD) is investigated numerically with the parameters of the HL-2A tokamak. Based on the understanding of the synergy mechanisms, a high current driven efficiency or a desired radial current profile can be achieved through properly matching the parameters of ECCD and LHCD due to the flexibility of ECCD. Meanwhile, it is found that the total current driven by the electron cyclotron wave (ECW) and the lower hybrid wave (LHW) simultaneously can be smaller than the sum of the currents driven by the ECW and LHW separately, when the power of the ECW is much larger than the LHW power. One of the reasons leading to this phenomenon (referred to as negative synergy in this context) is that fast current-carrying electrons tend to be trapped, when the perpendicular velocity driven by the ECW is large and the parallel velocity decided by the LHW is correspondingly small.

Startup and stability of a small spherical tokamak

NASA Astrophysics Data System (ADS)

Garstka, Gregory Douglas

1997-09-01

The spherical tokamak (ST) is an evolutionary extension of the conventional tokamak concept where the aspect ratio is less than 2. These devices may possess significant advantages over standard tokamaks-they are capable of achieving higher values of /beta, seem to be more resilient to disruptions, and are significantly smaller than conventional tokamaks. Two important questions for the next generation of spherical tokamaks concern startup and internal reconnection events (IREs). Understanding startup is crucial due to the limited amount of ohmic flux in an ST. The IREs are disruption- like events observed on STs that do not result in termination of the current channel. Experiments have been conducted on the Madison EDUcational Small Aspect-ratio (MEDUSA) tokamak to answer some of the questions about startup and IREs in STs. MEDUSA is a small ohmic tokamak with an insulating vacuum vessel. Major parameters are R=12 cm, a=8 cm, Ip=10-40 kA, BT=0.2-0.45 T, /Delta tpulse=1-2 ms, /langle ne/rangle/approx5×1019/ m-3, and Te0/approx100 eV. The experiments in this work were aided by an internal magnetic probe array that constrained the reconstruction of MHD equilibria. It was found that startup efficiency, measured by the Ejima coefficient CE, improved with increasing loop voltage and toroidal field. Double tearing modes were found to be an important mechanism for current penetration in MEDUSA; their presence early in the discharge can improve the magnetic flux consumption. The lowest achieved value of the Ejima coefficient was 0.61 (0.13 for 'OH only') for a discharge with 0.375 T toroidal field and 9.4 V startup loop voltage. The study of internal reconnection events revealed the presence of a heretofore undiscovered precursor, which in MEDUSA was manifested as coherent oscillations in the internal poloidal field at 65-75 kHz for 100 μs prior to the IRE. These events were found to result in decreased /ell i and /beta, inward movement of the magnetic axis, dramatically

Simultaneous IUE and Ground Based Observations of SS Cygni and HL Canis Majoris

NASA Astrophysics Data System (ADS)

Mansperger, C. S.; Kaitchuck, R. H.; Garnavich, P.; Dinshaw, N.

1993-05-01

SS Cyg and HL CMa were observed by IUE for three consecutive nights in November of 1992. During the first two nights, simultaneous photometric ground based observations of SS Cyg were made at the Ball State University Observatory. SS Cyg and HL CMa were observed simultaneously with the 90-inch telescope at the Steward Observatory on the last two nights of this run. These spectroscopic observations covered the wavelength range of 4100 Angstroms to 5000 Angstroms, while the spectra taken with the short wavelength camera on IUE resulted in wavelength coverage from 1150 Angstroms to 1980 Angstroms. SS Cyg is a U Gem type dwarf nova with an orbital period of 6.6 hours. Good simultaneous UV and optical orbital coverage was obtained for this system. HL CMa is a Z Cam type dwarf nova with an outburst period of 18 days. The AAVSO reports that this system was in outburst 4 days after the observing run. Therefore, HL CMa may have been in a preoutburst state during these observations. The C IV and H \\beta emission lines appeared to have weakened during this time.

Bifurcated helical core equilibrium states in tokamaks

NASA Astrophysics Data System (ADS)

Cooper, W. A.; Chapman, I. T.; Schmitz, O.; Turnbull, A. D.; Tobias, B. J.; Lazarus, E. A.; Turco, F.; Lanctot, M. J.; Evans, T. E.; Graves, J. P.; Brunetti, D.; Pfefferlé, D.; Reimerdes, H.; Sauter, O.; Halpern, F. D.; Tran, T. M.; Coda, S.; Duval, B. P.; Labit, B.; Pochelon, A.; Turnyanskiy, M. R.; Lao, L.; Luce, T. C.; Buttery, R.; Ferron, J. R.; Hollmann, E. M.; Petty, C. C.; van Zeeland, M.; Fenstermacher, M. E.; Hanson, J. M.; Lütjens, H.

2013-07-01

Tokamaks with weak to moderate reversed central shear in which the minimum inverse rotational transform (safety factor) qmin is in the neighbourhood of unity can trigger bifurcated magnetohydrodynamic equilibrium states, one of which is similar to a saturated ideal internal kink mode. Peaked prescribed pressure profiles reproduce the ‘snake’ structures observed in many tokamaks which has led to a novel explanation of the snake as a bifurcated equilibrium state. Snake equilibrium structures are computed in simulations of the tokamak à configuration variable (TCV), DIII-D and mega amp spherical torus (MAST) tokamaks. The internal helical deformations only weakly modulate the plasma-vacuum interface which is more sensitive to ripple and resonant magnetic perturbations. On the other hand, the external perturbations do not alter the helical core deformation in a significant manner. The confinement of fast particles in MAST simulations deteriorate with the amplitude of the helical core distortion. These three-dimensional bifurcated solutions constitute a paradigm shift that motivates the applications of tools developed for stellarator research in tokamak physics investigations.

Bootstrap current in a tokamak

DOE Office of Scientific and Technical Information (OSTI.GOV)

Kessel, C.E.

1994-03-01

The bootstrap current in a tokamak is examined by implementing the Hirshman-Sigmar model and comparing the predicted current profiles with those from two popular approximations. The dependences of the bootstrap current profile on the plasma properties are illustrated. The implications for steady state tokamaks are presented through two constraints; the pressure profile must be peaked and {beta}{sub p} must be kept below a critical value.

Inhibition of phosphatidylinositol 3-kinase causes apoptosis in retinoic acid differentiated hl-60 leukemia cells.

PubMed

Ma, Jin; Liu, Qiang; Zeng, Yi-Xin

2004-01-01

Phosphatidylinositol 3-kinase (PI3-K) signaling may inhibit apoptosis in neoplastic cells. The PI-3K inhibitor wortmannin renders cells apoptosis-prone. Inducers of differentiation may also cause apoptosis. To detect the effect of wortmannin on the survival of differentiated human acute promyeloid leukemia cells, HL-60 cells were induced to differentiation with treatment of all trans-retinoic acid (ATRA) followed by treatment with wortmannin. Results showed that apoptosis occurred in cells that underwent differentiation, but not in undifferentiated HL-60 cells. The pro-apoptotic molecule, Bad, played a role in this apoptotic mechanism. Thus, the survival of differentiated HL-60 cells induced by ATRA depends on the ability of the PI3-K pathway to transduce survival signals; the PI3-K inhibitor, wortmannin, can induce apoptosis of differentiated HL-60 cells. These results may indicate a novel method for treating cancer with differentiation induction and signal pathway regulation.

Characterization of plasma current quench during disruptions at HL-2A

NASA Astrophysics Data System (ADS)

Zhu, Jinxia; Zhang, Yipo; Dong, Yunbo; HL-2A Team

2017-05-01

The most essential assumptions of physics for the evaluation of electromagnetic forces on the plasma-facing components due to a disruption-induced eddy current are characteristics of plasma current quenches including the current quench rate or its waveforms. The characteristics of plasma current quenches at HL-2A have been analyzed during spontaneous disruptions. Both linear decay and exponential decay are found in the disruptions with the fastest current quenches. However, there are two stages of current quench in the slow current quench case. The first stage with an exponential decay and the second stage followed by a rapid linear decay. The faster current quench rate corresponds to the faster movement of plasma displacement. The parameter regimes on the current quench time and the current quench rates have been obtained from disruption statistics at HL-2A. There exists no remarkable difference for distributions obtained between the limiter and the divertor configuration. This data from HL-2A provides basic data of the derivation of design criteria for a large-sized machine during the current decay phase of the disruptions.

Physics of Tokamak Plasma Start-up

NASA Astrophysics Data System (ADS)

Mueller, Dennis

2012-10-01

This tutorial describes and reviews the state-of-art in tokamak plasma start-up and its importance to next step devices such as ITER, a Fusion Nuclear Science Facility and a Tokamak/ST demo. Tokamak plasma start-up includes breakdown of the initial gas, ramp-up of the plasma current to its final value and the control of plasma parameters during those phases. Tokamaks rely on an inductive component, typically a central solenoid, which has enabled attainment of high performance levels that has enabled the construction of the ITER device. Optimizing the inductive start-up phase continues to be an area of active research, especially in regards to achieving ITER scenarios. A new generation of superconducting tokamaks, EAST and KSTAR, experiments on DIII-D and operation with JET's ITER-like wall are contributing towards this effort. Inductive start-up relies on transformer action to generate a toroidal loop voltage and successful start-up is determined by gas breakdown, avalanche physics and plasma-wall interaction. The goal of achieving steady-sate tokamak operation has motivated interest in other methods for start-up that do not rely on the central solenoid. These include Coaxial Helicity Injection, outer poloidal field coil start-up, and point source helicity injection, which have achieved 200, 150 and 100 kA respectively of toroidal current on closed flux surfaces. Other methods including merging reconnection startup and Electron Bernstein Wave (EBW) plasma start-up are being studied on various devices. EBW start-up generates a directed electron channel due to wave particle interaction physics while the other methods mentioned rely on magnetic helicity injection and magnetic reconnection which are being modeled and understood using NIMROD code simulations.

Integrating radiology information systems with healthcare delivery environments using DICOM and HL7 standards.

PubMed

Blazona, Bojan; Koncar, Miroslav

2006-01-01

Integration based on open standards, in order to achieve communication and information interoperability, is one of the key aspects of modern health care information systems. Interoperability presents data and communication layer interchange. In this context we identified the HL7 standard as the world's leading medical Information and communication technology (ICT) standard for the business layer in healthcare information systems and we tried to explore the ability to exchange clinical documents with minimal integrated healthcare information systems (IHCIS) change. We explored HL7 Clinical Document Architecture (CDA) abilities to achieve radiology information system integration (DICOM) to IHCIS (HL7). We introduced the use of WADO service interconnection to IHCIS and finally CDA rendering in widely used Internet explorers.

Gene expression profiling for nitric oxide prodrug JS-K to kill HL-60 myeloid leukemia cells.

PubMed

Liu, Jie; Malavya, Swati; Wang, Xueqian; Saavedra, Joseph E; Keefer, Larry K; Tokar, Erik; Qu, Wei; Waalkes, Michael P; Shami, Paul J

2009-07-01

The nitric oxide (NO) prodrug JS-K is shown to have anticancer activity. To profile the molecular events associated with the anticancer effects of JS-K, HL-60 leukemia cells were treated with JS-K and subjected to microarray and real-time RT-PCR analysis. JS-K induced concentration- and time-dependent gene expression changes in HL-60 cells corresponding to the cytolethality effects. The apoptotic genes (caspases, Bax, and TNF-alpha) were induced, and differentiation-related genes (CD14, ITGAM, and VIM) were increased. For acute phase protein genes, some were increased (TP53, JUN) while others were suppressed (c-myc, cyclin E). The expression of anti-angiogenesis genes THBS1 and CD36 and genes involved in tumor cell migration such as tissue inhibitors of metalloproteinases, were also increased by JS-K. Confocal analysis confirmed key gene changes at the protein levels. Thus, multiple molecular events are associated with JS-K effects in killing HL-60, which could be molecular targets for this novel anticancer NO prodrug.

Magnetically Induced Disk Winds and Transport in the HL Tau Disk

NASA Astrophysics Data System (ADS)

Hasegawa, Yasuhiro; Okuzumi, Satoshi; Flock, Mario; Turner, Neal J.

2017-08-01

The mechanism of angular momentum transport in protoplanetary disks is fundamental to understanding the distributions of gas and dust in the disks. The unprecedented ALMA observations taken toward HL Tau at high spatial resolution and subsequent radiative transfer modeling reveal that a high degree of dust settling is currently achieved in the outer part of the HL Tau disk. Previous observations, however, suggest a high disk accretion rate onto the central star. This configuration is not necessarily intuitive in the framework of the conventional viscous disk model, since efficient accretion generally requires a high level of turbulence, which can suppress dust settling considerably. We develop a simplified, semi-analytical disk model to examine under what condition these two properties can be realized in a single model. Recent, non-ideal MHD simulations are utilized to realistically model the angular momentum transport both radially via MHD turbulence and vertically via magnetically induced disk winds. We find that the HL Tau disk configuration can be reproduced well when disk winds are properly taken into account. While the resulting disk properties are likely consistent with other observational results, such an ideal situation can be established only if the plasma β at the disk midplane is β 0 ≃ 2 × 104 under the assumption of steady accretion. Equivalently, the vertical magnetic flux at 100 au is about 0.2 mG. More detailed modeling is needed to fully identify the origin of the disk accretion and quantitatively examine plausible mechanisms behind the observed gap structures in the HL Tau disk.

Involvement of the histamine H{sub 4} receptor in clozapine-induced hematopoietic toxicity: Vulnerability under granulocytic differentiation of HL-60 cells

DOE Office of Scientific and Technical Information (OSTI.GOV)

Goto, Aya; Mouri, Akihiro; Nagai, Tomoko

Clozapine is an effective antipsychotic for treatment-resistant schizophrenia, but can cause fatal hematopoietic toxicity as agranulocytosis. To elucidate the mechanism of hematopoietic toxicity induced by clozapine, we developed an in vitro assay system using HL-60 cells, and investigated the effect on hematopoiesis. HL-60 cells were differentiated by all-trans retinoic acid (ATRA) into three states according to the following hematopoietic process: undifferentiated HL-60 cells, those undergoing granulocytic ATRA-differentiation, and ATRA-differentiated granulocytic cells. Hematopoietic toxicity was evaluated by analyzing cell survival, cell proliferation, granulocytic differentiation, apoptosis, and necrosis. In undifferentiated HL-60 cells and ATRA-differentiated granulocytic cells, both clozapine (50 and 100 μM)more » and doxorubicin (0.2 µM) decreased the cell survival rate, but olanzapine (1–100 µM) did not. Under granulocytic differentiation for 5 days, clozapine, even at a concentration of 25 μM, decreased survival without affecting granulocytic differentiation, increased caspase activity, and caused apoptosis rather than necrosis. Histamine H{sub 4} receptor mRNA was expressed in HL-60 cells, whereas the expression decreased under granulocytic ATRA-differentiation little by little. Both thioperamide, a histamine H{sub 4} receptor antagonist, and DEVD-FMK, a caspase-3 inhibitor, exerted protection against clozapine-induced survival rate reduction, but not of live cell counts. 4-Methylhistamine, a histamine H{sub 4} receptor agonist, decreased the survival rate and live cell counts, as did clozapine. HL-60 cells under granulocytic differentiation are vulnerable under in vitro assay conditions to hematopoietic toxicity induced by clozapine. Histamine H{sub 4} receptor is involved in the development of clozapine-induced hematopoietic toxicity through apoptosis, and may be a potential target for preventing its occurrence through granulocytic

Simulation of MST tokamak discharges with resonant magnetic perturbations

NASA Astrophysics Data System (ADS)

Cornille, B. S.; Sovinec, C. R.; Chapman, B. E.; Dubois, A.; McCollam, K. J.; Munaretto, S.

2016-10-01

Nonlinear MHD modeling of MST tokamak plasmas with an applied resonant magnetic perturbation (RMP) reveals degradation of flux surfaces that may account for the experimentally observed suppression of runaway electrons with the RMP. Runaway electrons are routinely generated in MST tokamak discharges with low plasma density. When an m = 3 RMP is applied these electrons are strongly suppressed, while an m = 1 RMP of comparable amplitude has little effect. The computations are performed using the NIMROD code and use reconstructed equilibrium states of MST tokamak plasmas with q (0) < 1 and q (a) = 2.2 . Linear computations show that the (1 , 1) -kink and (2 , 2) -tearing modes are unstable, and nonlinear simulations produce sawtoothing with a period of approximately 0.5 ms, which is comparable to the period of MHD activity observed experimentally. Adding an m = 3 RMP in the computation degrades flux surfaces in the outer region of the plasma, while no degradation occurs with an m = 1 RMP. The outer flux surface degradation with the m = 3 RMP, combined with the sawtooth-induced distortion of flux surfaces in the core, may account for the observed suppression of runaway electrons. Work supported by DOE Grant DE-FC02-08ER54975.

[Effects of baicalin on HL-60 cell xenografts in nude mice and its mechanism].

PubMed

Zheng, Jing; Hu, Jian-Da; Huang, Yi; Chen, Ying-Yu; Li, Jing; Chen, Bu-Yuan

2012-10-01

This study was aimed to investigate the effects of baicalin on HL-60 cell xenografts in nude mice in vivo and explore its mechanism. Xenograft tumor model of HL-60 cells in nude mice was established, which was divided randomly into 6 groups: negative control group (injection of 5% NaHCO(3)), 25, 50 and 100 mg/kg baicalin groups, combination group (50 mg/kg baicalin + 2 mg/kg VP16) and positive control group (VP16 4 mg/kg). The nude mice with HL-60 cell xenografts were treated with drugs via intraperitoneal injection daily. After treatment for 14 days average weigh and inhibitory rate of transplanted tumor stripped from 5 nude mice in each group were calculated, and the ultrastructure change of xenografts cells were tested by transmission electron microscopy. Histopathologic examination was used to observed the change of main organs in nude mice. The expression of signaling molecular PI3K/Akt proteins extracted from xenografts was detected by Western blot. The effects of baicalin on overall survival time in nude mice with HL-60 cell xenografts were evaluated. The results showed that baicalin could inhibit the growth of transplanted tumors in dose-dependent manner. There were more necrotic and apoptotic cells in mice of baicalin-treated groups and combination group than that in mice of negative control group. Baicalin could inhibit the proliferation of HL-60 cells in vivo by down-regulating the PI3K/Akt/mTOR signal pathway, where the expressions of p-Akt, mTOR and p-mTOR proteins decreased compared with negative control group, and no significant difference of Akt expression was found between different groups. Compared with negative control group, the median survival time of mice in combination group was more prolongated (P < 0.05). It is concluded that baicalin can inhibit growth and induce apoptosis of HL-60 cell xenografts in nude mice, and prolong median survival time of nude mice. The possible mechanisms may be related to inhibition of Akt activity and down

Numerical studies of edge localized instabilities in tokamaks

NASA Astrophysics Data System (ADS)

Wilson, H. R.; Snyder, P. B.; Huysmans, G. T. A.; Miller, R. L.

2002-04-01

A new computational tool, edge localized instabilities in tokamaks equilibria (ELITE), has been developed to help our understanding of short wavelength instabilities close to the edge of tokamak plasmas. Such instabilities may be responsible for the edge localized modes observed in high confinement H-mode regimes, which are a serious concern for next step tokamaks because of the high transient power loads which they can impose on divertor target plates. ELITE uses physical insight gained from analytic studies of peeling and ballooning modes to provide an efficient way of calculating the edge ideal magnetohydrodynamic stability properties of tokamaks. This paper describes the theoretical formalism which forms the basis for the code.

Physics evaluation of compact tokamak ignition experiments

DOE Office of Scientific and Technical Information (OSTI.GOV)

Uckan, N.A.; Houlberg, W.A.; Sheffield, J.

1985-01-01

At present, several approaches for compact, high-field tokamak ignition experiments are being considered. A comprehensive method for analyzing the potential physics operating regimes and plasma performance characteristics of such ignition experiments with O-D (analytic) and 1-1/2-D (WHIST) transport models is presented. The results from both calculations are in agreement and show that there are regimes in parameter space in which a class of small (R/sub o/ approx. 1-2 m), high-field (B/sub o/ approx. 8-13 T) tokamaks with aB/sub o/S/q/sub */ approx. 25 +- 5 and kappa = b/a approx. 1.6-2.0 appears ignitable for a reasonable range of transport assumptions. Consideringmore » both the density and beta limits, an evaluation of the performance is presented for various forms of chi/sub e/ and chi/sub i/, including degradation at high power and sawtooth activity. The prospects of ohmic ignition are also examined. 16 refs., 13 figs.« less

Effects of highly ripened cheeses on HL-60 human leukemia cells: antiproliferative activity and induction of apoptotic DNA damage.

PubMed

Yasuda, S; Ohkura, N; Suzuki, K; Yamasaki, M; Nishiyama, K; Kobayashi, H; Hoshi, Y; Kadooka, Y; Igoshi, K

2010-04-01

To establish cheese as a dairy product with health benefits, we examined the multifunctional role of cheeses. In this report, we clarify whether different types of commercial cheeses may possess antiproliferative activity using HL-60 human promyelocytic leukemia cell lines as a cancer model. Among 12 cheese extracts tested, 6 (Montagnard, Pont-l'Eveque, Brie, Camembert, Danablue, and Blue) revealed strong growth inhibition activity and induction of DNA fragmentation in HL-60 cells. Based on the quantification of nitrogen contents in different cheese samples, a positive correlation between the ripeness of various cheeses and their antiproliferative activity tested in HL-60 cells was displayed. Four varieties of Blue cheese ripened for 0, 1, 2, or 3 mo demonstrated that the Blue cheese ripened for a long term was capable of causing the strong suppression of the cell growth and the induction of apoptotic DNA damage as well as nucleic morphological change in HL-60 cells. Collectively, these results obtained suggest a potential role of highly ripened cheeses in the prevention of leukemic cell proliferation. Copyright (c) 2010 American Dairy Science Association. Published by Elsevier Inc. All rights reserved.

Development of an HL7 interface engine, based on tree structure and streaming algorithm, for large-size messages which include image data.

PubMed

Um, Ki Sung; Kwak, Yun Sik; Cho, Hune; Kim, Il Kon

2005-11-01

A basic assumption of Health Level Seven (HL7) protocol is 'No limitation of message length'. However, most existing commercial HL7 interface engines do limit message length because they use the string array method, which is run in the main memory for the HL7 message parsing process. Specifically, messages with image and multi-media data create a long string array and thus cause the computer system to raise critical and fatal problem. Consequently, HL7 messages cannot handle the image and multi-media data necessary in modern medical records. This study aims to solve this problem with the 'streaming algorithm' method. This new method for HL7 message parsing applies the character-stream object which process character by character between the main memory and hard disk device with the consequence that the processing load on main memory could be alleviated. The main functions of this new engine are generating, parsing, validating, browsing, sending, and receiving HL7 messages. Also, the engine can parse and generate XML-formatted HL7 messages. This new HL7 engine successfully exchanged HL7 messages with 10 megabyte size images and discharge summary information between two university hospitals.

From pure fusion to fusion-fission Demo tokamaks

NASA Astrophysics Data System (ADS)

Mirnov, S. V.

2013-04-01

The major requirements for pure fusion tokamak reactors and tokamak-based fusion neutron sources (FNS) are analyzed together with possible paths from the present-day tokamak towards the FNS tokamak. The FNS are of interest for traditional fission reactors as a method of waste management by burning of long-lived transuranic radionuclides (minorities) and fission fuel breeding. The Russian fission community places several hard requirements on the quality of FNS suitable for the first step of the investigation program of minority burning and breeding. They are (a) a steady-state regime of neutron production (more than 80% of the operational time), (b) a neutron power flux density greater than >0.2 MW m-2, (c) a total surface integrated neutron power >10 MW. Among the different FNS projects, based on magnetically confined plasmas, only ‘classical tokamak’ is most likely to fulfill these requirements in the nearest future. Some of the most important improvements of the ‘classical tokamak’ needed for successful realization of the FNS are (1) decrease in Zeff (probably, by making use of lithium as a part of plasma-facing components), (2) He removal and closed loop DT fuel circulation, (3) increase in the energy of stationary injected neutral tritium beams up to 150-170 keV and (4) control of impurity contamination at the plasma center (probably, by local RF heating). These key issues are discussed.

Effects of Simulated Microgravity on Functions of Neutrophil-like HL-60 Cells

NASA Astrophysics Data System (ADS)

Wang, Chengzhi; Li, Ning; Zhang, Chen; Sun, Shujin; Gao, Yuxin; Long, Mian

2015-11-01

Altered gravity, especially microgravity affects cellular functions of immune cells and can result in immune dysfunction for long-term, manned spaceflight and space exploration. The underlying mechanism, however, of sensing and responding to the gravity alteration is poorly understood. Here, a rotary cell culture system (RCCS) bioreactor was used to elucidate the effects of simulated microgravity on polymorphonuclear neutrophils (PMN)-like HL-60 cells. Alteration of cell morphology, up-regulation of (nitric oxide) NO production, enhancement of interleukin-6 (IL-6), interleukin-8 (IL-8), and monocyte chemotactic protein 1 (MCP-1) secretion, and diversity of cellular adhesion molecule expression were observed for the cells cultured in RCCS, leading to the up-regulated inflammatory immune responses and host defense. It was also indicated that such alterations in biological responses of PMNs mediated the reduced rolling velocity and decreased adhesion of PMN-like HL-60 cells on endothelial cells under shear flow. This work furthers the understandings in the effects and mechanism of microgravity on PMN functions, which are potentially helpful for optimizing the countermeasures to immune suppression in the future long-term, manned spaceflight.

An Effective Model of the Retinoic Acid Induced HL-60 Differentiation Program.

PubMed

Tasseff, Ryan; Jensen, Holly A; Congleton, Johanna; Dai, David; Rogers, Katharine V; Sagar, Adithya; Bunaciu, Rodica P; Yen, Andrew; Varner, Jeffrey D

2017-10-30

In this study, we present an effective model All-Trans Retinoic Acid (ATRA)-induced differentiation of HL-60 cells. The model describes reinforcing feedback between an ATRA-inducible signalsome complex involving many proteins including Vav1, a guanine nucleotide exchange factor, and the activation of the mitogen activated protein kinase (MAPK) cascade. We decomposed the effective model into three modules; a signal initiation module that sensed and transformed an ATRA signal into program activation signals; a signal integration module that controlled the expression of upstream transcription factors; and a phenotype module which encoded the expression of functional differentiation markers from the ATRA-inducible transcription factors. We identified an ensemble of effective model parameters using measurements taken from ATRA-induced HL-60 cells. Using these parameters, model analysis predicted that MAPK activation was bistable as a function of ATRA exposure. Conformational experiments supported ATRA-induced bistability. Additionally, the model captured intermediate and phenotypic gene expression data. Knockout analysis suggested Gfi-1 and PPARg were critical to the ATRAinduced differentiation program. These findings, combined with other literature evidence, suggested that reinforcing feedback is central to hyperactive signaling in a diversity of cell fate programs.

Molecular characterization of Coriolus versicolor PSP-induced apoptosis in human promyelotic leukemic HL-60 cells using cDNA microarray.

PubMed

Zeng, Fanya; Hon, Chung-Chau; Sit, Wai-Hung; Chow, Ken Yan-Ching; Hui, Raymond Kin-Hi; Law, Ivy Ka-Man; Ng, Victor Wai-Lap; Yang, Xiao-Tong; Leung, Frederick Chi-Ching; Wan, Jennifer Man-Fan

2005-08-01

Proteins and peptide bound polysaccharides (PSP) extracted from Basidiomycetous fungi are widely used in cancer immunotherapy and recently demonstrated to induce apoptosis in cancer cells in vitro. In order to provide the molecular pharmacological mechanisms of PSP on human cancer cells, we investigated the gene expression profiles of PSP-treated apoptotic human promyelotic leukemic HL-60 cells using ResGen 40k IMAGE printed cDNA microarray. In total 378 and 111 transcripts were identified as differentially expressed in the apoptotic cells by at least a factor of 2 or 3, respectively. Our data show that PSP-induced apoptosis in HL-60 cells might be mediated by up-regulation of early transcription factors such as AP-1, EGR1, IER2 and IER5, and down-regulation of NF-kappaB transcription pathways. Other gene expression changes, including the increase of several apoptotic or anti-proliferation genes, such as GADD45A/B and TUSC2, and the decrease of a batch of phosphatase and kinase genes, may also provide further evidences in supporting the process of PSP induced apoptosis in cancer cells. Some of the well-characterized carcinogenesis-related gene transcripts such as SAT, DCT, Melan-A, uPA and cyclin E1 were also alternated by PSP in the HL-60 cells. These transcripts can be employed as markers for quality control of PSP products on functional levels. The present study provides new insight into the molecular mechanisms involved in PSP-induced apoptosis in leukemic HL-60 cells analyzed by cDNA microarray.

Controlling fusion yield in tokamaks with spin polarized fuel, and feasibility studies on the DIII-D tokamak

DOE PAGES

Pace, D. C.; Lanctot, M. J.; Jackson, G. L.; ...

2015-09-21

The march towards electricity production through tokamaks requires the construction of new facilities and the inevitable replacement of the previous generation. There are, however, research topics that are better suited to the existing tokamaks, areas of great potential that are not sufficiently mature for implementation in high power machines, and these provide strong support for a balanced policy that includes the redirection of existing programs. Spin polarized fusion, in which the nuclei of tokamak fuel particles are spin-aligned and favorably change both the fusion cross-section and the distribution of initial velocity vectors of charged fusion products, is described here asmore » an example of a technological and physics topic that is ripe for development in a machine such as the DIII-D tokamak. In this study, such research and development experiments may not be efficient at the ITER-scale, while the plasma performance, diagnostic access, and collaborative personnel available within the United States’ magnetic fusion research program, and at the DIII-D facility in particular, provide a unique opportunity to further fusion progress.« less

Leucine aminopeptidase, HlLAP, from the ixodid tick Haemaphysalis longicornis, plays vital roles in the development of oocytes.

PubMed

Hatta, Takeshi; Tsuji, Naotoshi; Miyoshi, Takeharu; Islam, M Khyrul; Alim, M Abdul; Yamaji, Kayoko; Anisuzzaman; Fujisaki, Kozo

2010-06-01

Female ixodid ticks are amazing invertebrate animals which efficiently convert a large amount of nutrients derived from their ingested blood meals into eggs. Although oocyte development (vitellogenesis) in ticks is triggered by a blood meal and is assumed to be supported by nutrition derived from ovarian cells connecting oocytes, little is known about the ovarian molecules processing nutrient materials for the vitellogenesis. In this study, we have suggested a putative function of leucine aminopeptidase (HlLAP) in the ovary of parthenogenetic adult ixodid tick Haemaphysalis longicornis regarding a negative output of reproduction following disruption of HlLAP gene by RNA interference. Endogenous HlLAP was shown to be localized in the ovarian cells, including ovarian epithelial and pedicel cells which were assumed to provide nutrients for the developing oocytes. Histological studies demonstrated that a majority of immature oocytes in HlLAP gene knockdown ticks were transformed into abnormal morpho-histological oocytes with vacuolated cytoplasm and/or condensed nucleus. Taken together, a reduction of the numbers of laid eggs in the HlLAP gene knockdown ticks may be due to the degeneration of immature oocytes following deprivation of nutrients such as amino acids supplied not only by midgut HlLAP but also by the ovarian HlLAP. Regulation of the tick molecules involved in nutrient metabolism for the reproduction, including blood digestion and vitellogenesis, would help in controlling the tick population and tick-borne pathogens.

Citrus flavone tangeretin inhibits leukaemic HL-60 cell growth partially through induction of apoptosis with less cytotoxicity on normal lymphocytes.

PubMed

Hirano, T; Abe, K; Gotoh, M; Oka, K

1995-12-01

Certain anti-cancer agents are known to induce apoptosis in human tumour cells. However, these agents are intrinsically cytotoxic against cells of normal tissue origin, including myelocytes and immunocytes. Here we show that a naturally occurring flavone of citrus origin, tangeretin (5,6,7,8,4'-pentamethoxyflavone), induces apoptosis in human promyelocytic leukaemia HL-60 cells, whereas the flavone showed no cytotoxicity against human peripheral blood mononuclear cells (PBMCs). The growth of HL-60 cells in vitro assessed by [3H]thymidine incorporation or tetrazolium crystal formation was strongly suppressed in the presence of tangeretin; the IC50 values range between 0.062 and 0.173 microM. Apoptosis of HL-60 cells, assessed by cell morphology and DNA fragmentation, was demonstrated in the presence of > 2.7 microM tangeretin. Flow cytometric analysis of tangeretin-treated HL-60 cells also demonstrated apoptotic cells with low DNA content and showed a decrease of G1 cells and a concomitant increase of S and/or G2/M cells. Apoptosis was evident after 24 h of incubation with tangeretin, and the tangeretin effect as assessed by DNA fragmentation or growth inhibition was significantly attenuated in the presence of Zn2+, which is known to inhibit Ca(2+)-dependent endonuclease activity. Ca2+ and Mg2+, in contrast, promoted the effect of tangeretin. Cycloheximide significantly decreased the tangeretin effect on HL-60 cell growth, suggesting that protein synthesis is required for flavonoid-induced apoptosis. Tangeretin showed no cytotoxicity against either HL-60 cells or mitogen-activated PBMCs even at high concentration (27 microM) as determined by a dye exclusion test. Moreover, the flavonoid was less effective on growth of human T-lymphocytic leukaemia MOLT-4 cells or on blastogenesis of PBMCs. These results suggest that tangeretin inhibits growth of HL-60 cells in vitro, partially through induction of apoptosis, without causing serious side-effects on immune cells.

In vitro anticancer property of a novel thalidomide analogue through inhibition of NF-kappaB activation in HL-60 cells.

PubMed

Li, Min; Sun, Wan; Yang, Ya-ping; Xu, Bo; Yi, Wen-yuan; Ma, Yan-xia; Li, Zhong-jun; Cui, Jing-rong

2009-01-01

To investigate the anticancer property and possible mechanism of action of a novel sugar-substituted thalidomide derivative (STA-35) on HL-60 cells in vitro. TNF-alpha-induced NF-kappaB activation was determined using a reporter gene assay. The MTT assay was used to measure cytotoxicity of the compound. The appearance of apoptotic Sub-G1 cells was detected by flow cytometry analysis. PARP cleavage and protein expression of NF-kappaB p65 and its inhibitor IkappaB were viewed by Western blotting. TA-35 (1-20 micromol/L) suppressed TNF-alpha-induced NF-kappaB activation in transfected cells (HEK293/pNiFty-SEAP) in a dose- (1-20 micromol/L) and time-dependent (0-48 h) manner. It was also shown that STA-35 exerted a dose-dependent inhibitory effect on HL-60 cell proliferation with an IC(50) value of 9.05 micromol/L. In addition, STA-35 induced apoptosis in HL-60 cells, as indicated by the appearance of a Sub-G1 peak in the cell cycle distribution, as well as poly ADP-ribose polymerase (PARP) cleavage. Subsequently, both NF-kappaB p65 and its inhibitor IkappaB gradually accumulated in cytoplasmic extracts in a dose- and time-dependent manner, indicating the blockage of NF-kappaB translocation induced by TNF-alpha from the cytoplasm to the nucleus. A novel sugar-substituted thalidomide derivative, STA-35, is potent toward HL-60 cells in vitro and induces apoptosis by the suppression of NF-kappaB activation.

Expression pattern and signalling pathways in neutrophil like HL-60 cells after treatment with estrogen receptor selective ligands.

PubMed

Blesson, Chellakkan Selvanesan; Sahlin, Lena

2012-09-25

Estrogens play a role in the regulation of genes associated with inflammation and immunity in neutrophils. Estrogen signalling is mediated by estrogen receptor (ER)α, ERβ, and G-protein-coupled estrogen receptor-1 (GPER). The mechanisms by which estrogen regulate genes in neutrophils are poorly understood. Our aim was to identify the presence of ERs and to characterize estrogen responsive genes in terminally differentiated neutrophil like HL-60 (nHL-60) cells using estradiol and selective ER agonists. ERs were identified by Western blotting and immunocytochemistry. Microarray technique was used to screen for differentially expressed genes and the selected genes were verified by quantitative PCR. We show the presence of functional ERα, ERβ and GPER. Microarray analysis showed the presence of genes that are uniquely regulated by a single ligand and also genes that are regulated by multiple ligands. We conclude that ERs are functionally active in nHL-60 cells regulating genes involved in key physiological functions. Copyright © 2012 Elsevier Ireland Ltd. All rights reserved.

What is the fate of runaway positrons in tokamaks?

DOE PAGES

Liu, Jian; Qin, Hong; Fisch, Nathaniel J.; ...

2014-06-19

In this study, massive runaway positrons are generated by runaway electrons in tokamaks. The fate of these positrons encodes valuable information about the runaway dynamics. The phase space dynamics of a runaway position is investigated using a Lagrangian that incorporates the tokamak geometry, loop voltage, radiation and collisional effects. It is found numerically that runaway positrons will drift out of the plasma to annihilate on the first wall, with an in-plasma annihilation possibility less than 0.1%. The dynamics of runaway positrons provides signatures that can be observed as diagnostic tools.

What is the fate of runaway positrons in tokamaks?

DOE Office of Scientific and Technical Information (OSTI.GOV)

Liu, Jian; Qin, Hong, E-mail: hongqin@ustc.edu.cn; Princeton Plasma Physics Laboratory, Princeton University, Princeton, New Jersey 08543

2014-06-15

Massive runaway positrons are generated by runaway electrons in tokamaks. The fate of these positrons encodes valuable information about the runaway dynamics. The phase space dynamics of a runaway position is investigated using a Lagrangian that incorporates the tokamak geometry, loop voltage, radiation and collisional effects. It is found numerically that runaway positrons will drift out of the plasma to annihilate on the first wall, with an in-plasma annihilation possibility less than 0.1%. The dynamics of runaway positrons provides signatures that can be observed as diagnostic tools.

Magnetically Induced Disk Winds and Transport in the HL Tau Disk

DOE Office of Scientific and Technical Information (OSTI.GOV)

Hasegawa, Yasuhiro; Flock, Mario; Turner, Neal J.

2017-08-10

The mechanism of angular momentum transport in protoplanetary disks is fundamental to understanding the distributions of gas and dust in the disks. The unprecedented ALMA observations taken toward HL Tau at high spatial resolution and subsequent radiative transfer modeling reveal that a high degree of dust settling is currently achieved in the outer part of the HL Tau disk. Previous observations, however, suggest a high disk accretion rate onto the central star. This configuration is not necessarily intuitive in the framework of the conventional viscous disk model, since efficient accretion generally requires a high level of turbulence, which can suppressmore » dust settling considerably. We develop a simplified, semi-analytical disk model to examine under what condition these two properties can be realized in a single model. Recent, non-ideal MHD simulations are utilized to realistically model the angular momentum transport both radially via MHD turbulence and vertically via magnetically induced disk winds. We find that the HL Tau disk configuration can be reproduced well when disk winds are properly taken into account. While the resulting disk properties are likely consistent with other observational results, such an ideal situation can be established only if the plasma β at the disk midplane is β {sub 0} ≃ 2 × 10{sup 4} under the assumption of steady accretion. Equivalently, the vertical magnetic flux at 100 au is about 0.2 mG. More detailed modeling is needed to fully identify the origin of the disk accretion and quantitatively examine plausible mechanisms behind the observed gap structures in the HL Tau disk.« less

Development of frequency modulation reflectometer for Korea Superconducting Tokamak Advanced Research tokamak

DOE Office of Scientific and Technical Information (OSTI.GOV)

Seo, Seong-Heon; Wi, H. M.; Lee, W. R.

2013-08-15

Frequency modulation reflectometer has been developed to measure the plasma density profile of the Korea Superconducting Tokamak Advanced Research tokamak. Three reflectometers are operating in extraordinary polarization mode in the frequency range of Q band (33.6–54 GHz), V band (48–72 GHz), and W band (72–108 GHz) to measure the density up to 7 × 10{sup 19} m{sup −3} when the toroidal magnetic field is 2 T on axis. The antenna is installed inside of the vacuum vessel. A new vacuum window is developed by using 50 μm thick mica film and 0.1 mm thick gold gasket. The filter bank ofmore » low pass filter, notch filter, and Faraday isolator is used to reject the electron cyclotron heating high power at attenuation of 60 dB. The full frequency band is swept in 20 μs. The mixer output is directly digitized with sampling rate of 100 MSamples/s. The phase is obtained by using wavelet transform. The whole hardware and software system is described in detail and the measured density profile is presented as a result.« less

Circumstellar gas associated with HL Tauri - Evidence for a remnant infalling envelope

NASA Technical Reports Server (NTRS)

Grasdalen, Gary L.; Sloan, Gregory; Stout, Natalie; Strom, Stephen E.; Welty, Alan D.

1989-01-01

Molecular carbon absorption lines in the spectrum of HL Tau has been discovered near 8775 A. These C2 lines have a heliocentric radial velocity of 43 + or - 3 km/s, redshifted by 23 + or - 3 km/s relative to the star and the associated molecular cloud. This velocity difference suggests that the molecular carbon absorption arises in an infalling envelope. Since KI and diffuse interstellar bands are much weaker than expected, the chemical composition and/or excitation conditions in the HL Tau envelope appear to differ substantially from those in the interstellar medium.

Hollow Electron Beam Collimation for HL-LHC - Effects on the Beam Core

DOE Office of Scientific and Technical Information (OSTI.GOV)

Fitterer, M.; Stancari, G.; Valishev, A.

2017-06-13

Collimation with hollow electron beams is currently one of the most promising concepts for active halo control in the High Luminosity Large Hadron Collider (HL-LHC). To ensure the successful operation of the hollow beam collimator the unwanted effects on the beam core, which might arise from the operation with a pulsed electron beam, must be minimized. This paper gives a summary of the effect of hollow electron lenses on the beam core in terms of sources, provides estimates for HL-LHC and discusses the possible mitigation methods.

MHD Instabilities and Toroidal Field Effects on Plasma Column Behavior in Tokamak

DOE Office of Scientific and Technical Information (OSTI.GOV)

Khorshid, Pejman; Plasma Physics Research Center, Islamic Azad University, 14665-678, Tehran; Wang, L.

2006-12-04

In the edge plasma of the CT-6B and IRAN-T1 tokamaks the shape of plasma column based on MHD behavior has been studied. The bulk of plasma behavior during plasma column rotation as non-rigid body plasma has been investigated. We found that mode number and rotation frequency of plasma column are different in angle position, so that the mode number detected from Mirnov coils array located in poloidal angle on the inner side of chamber is more than outer side which it can be because of toroidal magnetic field effects. The results of IR-T1 and CT-6B tokamaks compared with each other,more » so that in the CT-6B because of its coils number must be less, but because of its Iron core the effect of toroidal magnetic field became more effective with respect to IR-T1. In addition, it is shown that the plasma column behaves as non-Rigid body plasma so that the poloidal rotation velocity variation in CT-6B is more than IR-T1. A relative correction for island rotation frequency has been suggested in connection with IRAN-T1 and CT-6B tokamak results, which can be considered for optical measurement purposes and also for future advanced tokamak control design.« less

L1 track trigger for the CMS HL-LHC upgrade using AM chips and FPGAs

NASA Astrophysics Data System (ADS)

Fedi, Giacomo

2017-08-01

The increase of luminosity at the HL-LHC will require the introduction of tracker information in CMS's Level-1 trigger system to maintain an acceptable trigger rate when selecting interesting events, despite the order of magnitude increase in minimum bias interactions. To meet the latency requirements, dedicated hardware has to be used. This paper presents the results of tests of a prototype system (pattern recognition ezzanine) as core of pattern recognition and track fitting for the CMS experiment, combining the power of both associative memory custom ASICs and modern Field Programmable Gate Array (FPGA) devices. The mezzanine uses the latest available associative memory devices (AM06) and the most modern Xilinx Ultrascale FPGAs. The results of the test for a complete tower comprising about 0.5 million patterns is presented, using as simulated input events traversing the upgraded CMS detector. The paper shows the performance of the pattern matching, track finding and track fitting, along with the latency and processing time needed. The pT resolution over pT of the muons measured using the reconstruction algorithm is at the order of 1% in the range 3-100 GeV/c.

Synergistic induction of 1,25-dihydroxyvitamin D(3)- and all-trans-retinoic acid-induced differentiation of HL-60 leukemia cells by yomogin, a sesquiterpene lactone from Artemisia princeps.

PubMed

Kim, Seung Hyun; Kim, Tae Sung

2002-10-01

Many anti-inflammatory agents are known to significantly enhance the terminal differentiation of some cancer cells such as leukemia cells. In this study, the effect of yomogin, a eudesmane sesquiterpene lactone isolated from Artemisia princeps with anti-inflammatory activity, was investigated in human promyelocytic leukemia HL-60 cells. Yomogin by itself induced small increases in cell differentiation, with less than 19 % of the cells attaining a differentiated phenotype. Importantly, yomogin synergistically enhanced differentiation of HL-60 cells in a dose-dependent manner when combined with either 5 nM 1,25-dihydroxyvitamin D(3) [1,25-(OH)(2) D(3)] or 50 nM all- trans retinoic acid (all- trans RA). Cytofluorometric analysis and morphologic studies indicated that the combinations of yomogin and 1,25-(OH)(2) D(3) stimulated differentiation to monocytes whereas the combinations of yomogin and all- trans RA stimulated differentiation to granulocytes. These results suggest that yomogin may be useful in combination with 1,25-(OH)(2) D(3) or all- trans-RA in the differentiation therapy for myeloid leukemias. Abbreviations. 1,25-(OH)(2) D(3) :1,25-dihydroxyvitamin D(3) FITC:fluorescein isothiocyanate NBT:nitroblue tetrazolium RA:retinoic acid PE:phytoerythrin

HL-20 operations and support requirements for the Personnel Launch System mission

NASA Technical Reports Server (NTRS)

Morris, W. D.; White, Nancy H.; Caldwell, Ronald G.

1993-01-01

The processing, mission planning, and support requirements were defined for the HL-20 lifting-body configuration that can serve as a Personnel Launch System. These requirements were based on the assumption of an operating environment that incorporates aircraft and airline support methods and techniques that are applicable to operations. The study covered the complete turnaround process for the HL-20, including landing through launch, and mission operations, but did not address the support requirements of the launch vehicle except for the integrated activities. Support is defined in terms of manpower, staffing levels, facilities, ground support equipment, maintenance/sparing requirements, and turnaround processing time. Support results were drawn from two contracted studies, plus an in-house analysis used to define the maintenance manpower. The results of the contracted studies were used as the basis for a stochastic simulation of the support environment to determine the sufficiency of support and the effect of variance on vehicle processing. Results indicate the levels of support defined for the HL-20 through this process to be sufficient to achieve the desired flight rate of eight flights per year.

New botanical drug, HL tablet, reduces hepatic fat as measured by magnetic resonance spectroscopy in patients with nonalcoholic fatty liver disease: A placebo-controlled, randomized, phase II trial.

PubMed

Jeong, Jae Yoon; Sohn, Joo Hyun; Baek, Yang Hyun; Cho, Yong Kyun; Kim, Yongsoo; Kim, Hyeonjin

2017-08-28

To evaluate the efficacy and safety of HL tablet extracted from magnolia officinalis for treating patients with nonalcoholic fatty liver disease (NAFLD). Seventy-four patients with NAFLD diagnosed by ultrasonography were randomly assigned to 3 groups given high dose (400 mg) HL tablet, low dose (133.4 mg) HL tablet and placebo, respectively, daily for 12 wk. The primary endpoint was post-treatment change of hepatic fat content (HFC) measured by magnetic resonance spectroscopy. Secondary endpoints included changes of serum aspartate aminotransferase, alanine aminotransferase (ALT), cholesterol, triglyceride, free fatty acid, homeostasis model assessment-estimated insulin resistance, and body mass index (BMI). The mean HFC of the high dose HL group, but not of the low dose group, declined significantly after 12 wk of treatment (high dose vs placebo, P = 0.033; low dose vs placebo, P = 0.386). The mean changes of HFC from baseline at week 12 were -1.7% ± 3.1% in the high dose group ( P = 0.018), -1.21% ± 4.97% in the low dose group ( P = 0.254) and 0.61% ± 3.87% in the placebo group (relative changes compared to baseline, high dose were: -12.1% ± 23.5%, low dose: -3.2% ± 32.0%, and placebo: 7.6% ± 44.0%). Serum ALT levels also tended to decrease in the groups receiving HL tablet while other factors were unaffected. There were no moderate or severe treatment-related safety issues during the study. HL tablet is effective in reducing HFC without any negative lipid profiles, BMI changes and adverse effects.

Investigation of peeling-ballooning stability prior to transient outbursts accompanying transitions out of H-mode in DIII-D

DOE Office of Scientific and Technical Information (OSTI.GOV)

Eldon, David; Boivin, Rejean L.; Groebner, Richard J.

Here, the H-mode transport barrier allows confinement of roughly twice as much energy as in an L-mode plasma. Termination of H-mode necessarily requires release of this energy, and the timescale of that release is of critical importance for the lifetimes of plasma facing components in next step tokamaks such as ITER. H-L transition sequences in modern tokamaks often begin with a transient outburst which appears to be superficially similar to and has sometimes been referred to as a type-I edge localized mode (ELM). Type-I ELMs have been shown to be consistent with ideal peeling ballooning instability and are characterized bymore » significant (up to ~50%) reduction of pedestal height on short (~1 ms) timescales. Knowing whether or not this type of instability is present during H-L back transitions will be important for planning for plasma ramp-down in ITER. This paper presents tests of pre-transition experimental data against ideal peeling-ballooning stability calculations with the ELITE code and supports 2 those results with secondary experiments that together show that the transient associated with the H-L transition is not triggered by the same physics as are type-I ELMs.« less

Investigation of peeling-ballooning stability prior to transient outbursts accompanying transitions out of H-mode in DIII-D

DOE Office of Scientific and Technical Information (OSTI.GOV)

Eldon, D., E-mail: deldon@princeton.edu; Princeton University, Princeton, New Jersey 08543; Boivin, R. L.

The H-mode transport barrier allows confinement of roughly twice as much energy as in an L-mode plasma. Termination of H-mode necessarily requires release of this energy, and the timescale of that release is of critical importance for the lifetimes of plasma facing components in next step tokamaks such as ITER. H-L transition sequences in modern tokamaks often begin with a transient outburst which appears to be superficially similar to and has sometimes been referred to as a type-I edge localized mode (ELM). Type-I ELMs have been shown to be consistent with ideal peeling ballooning instability and are characterized by significantmore » (up to ∼50%) reduction of pedestal height on short (∼1 ms) timescales. Knowing whether or not this type of instability is present during H-L back transitions will be important of planning for plasma ramp-down in ITER. This paper presents tests of pre-transition experimental data against ideal peeling-ballooning stability calculations with the ELITE code and supports those results with secondary experiments that together show that the transient associated with the H-L transition is not triggered by the same physics as are type-I ELMs.« less

Investigation of peeling-ballooning stability prior to transient outbursts accompanying transitions out of H-mode in DIII-D

DOE PAGES

Eldon, David; Boivin, Rejean L.; Groebner, Richard J.; ...

2015-05-14

Here, the H-mode transport barrier allows confinement of roughly twice as much energy as in an L-mode plasma. Termination of H-mode necessarily requires release of this energy, and the timescale of that release is of critical importance for the lifetimes of plasma facing components in next step tokamaks such as ITER. H-L transition sequences in modern tokamaks often begin with a transient outburst which appears to be superficially similar to and has sometimes been referred to as a type-I edge localized mode (ELM). Type-I ELMs have been shown to be consistent with ideal peeling ballooning instability and are characterized bymore » significant (up to ~50%) reduction of pedestal height on short (~1 ms) timescales. Knowing whether or not this type of instability is present during H-L back transitions will be important for planning for plasma ramp-down in ITER. This paper presents tests of pre-transition experimental data against ideal peeling-ballooning stability calculations with the ELITE code and supports 2 those results with secondary experiments that together show that the transient associated with the H-L transition is not triggered by the same physics as are type-I ELMs.« less

UCLA Tokamak Program Close Out Report.

DOE Office of Scientific and Technical Information (OSTI.GOV)

Taylor, Robert John

2014-02-04

The results of UCLA experimental fusion program are summarized. Starting with smaller devices like Microtor, Macrotor, CCT and ending the research on the large (5 m) Electric Tokamak. CCT was the most diagnosed device for H-mode like physics and the effects of rotation induced radial fields. ICRF heating was also studied but plasma heating of University Type Tokamaks did not produce useful results due to plasma edge disturbances of the antennae. The Electric Tokamak produced better confinement in the seconds range. However, it presented very good particle confinement due to an "electric particle pinch". This effect prevented us from reachingmore » a quasi steady state. This particle accumulation effect was numerically explained by Shaing's enhanced neoclassical theory. The PI believes that ITER will have a good energy confinement time but deleteriously large particle confinement time and it will disrupt on particle pinching at nominal average densities. The US fusion research program did not study particle transport effects due to its undue focus on the physics of energy confinement time. Energy confinement time is not an issue for energy producing tokamaks. Controlling the ash flow will be very expensive.« less

MHD Studies of Advanced Tokamak Equilibria

NASA Astrophysics Data System (ADS)

Strumberger, E.

2005-10-01

Advanced tokamak scenarios are often characterized by an extremely reversed profile of the safety factor, q, and a fast toroidal rotation. ASDEX Upgrade type equilibria with toroidal flow are computed up to a toroidal Mach number of Mta= 0.5, and compared with the static solution. Using these equilibria, the stabilizing effect of differential toroidal rotation on double tearing modes (DTMs) is investigated. These studies show that the computation of equilibria with flow is necessary for toroidally rotating plasma with Mta>=0.2. The use of ρtor instead of ρpol as radial coordinate enables us also to investigate the stability of equilibria with current holes. For numerical reasons, the rotational transform, = 1/q, has to be unequal zero in the CASTOR$FLOW code, but values of a>=0.001 (qa<=1000) can be easily handled. Stability studies of DTMs in the presence of a current hole are presented. Tokamak equilibria are only approximately axisymmetric. The finite number of toroidal field coils destroys the perfect axisymmetry of the device, and the coils produce a short wavelength ripple in the magnetic field strength. This toroidal field ripple plays a crucial role for the loss of high energy particles. Therefore, three-dimensional tokamak equilibria with and without current holes are computed for various plasma beta values. In addition the influence of the plasma beta on the toroidal field ripple is investigated.

Spherical tokamaks with plasma centre-post

NASA Astrophysics Data System (ADS)

Ribeiro, Celso

2013-10-01

The metal centre-post (MCP) in tokamaks is a structure which carries the total toroidal field current and also houses the Ohmic heating solenoid in conventional or low aspect ratio (Spherical)(ST) tokamaks. The MCP and solenoid are critical components for producing the toroidal field and for the limited Ohmic flux in STs. Constraints for a ST reactor related to these limitations lead to a minimum plasma aspect ratio of 1.4 which reduces the benefit of operation at higher betas in a more compact ST reactor. Replacing the MCP is of great interest for reactor-based ST studies since the device is simplified, compactness increased, and maintenance reduced. An experiment to show the feasibility of using a plasma centre-post (PCP) is being currently under construction and involves a high level of complexity. A preliminary study of a very simple PCP, which is ECR(Electron Cyclotron Resonance)-assisted and which includes an innovative fuelling system based on pellet injection, has recently been reported. This is highly suitable for an ultra-low aspect ratio tokamak (ULART) device. Advances on this PCP ECR-assisted concept within a ULART and the associated fuelling system are presented here, and will include the field topology for the PCP ECR-assisted scheme, pellet ablation modeling, and a possible global equilibrium simulation. VIE-ITCR, IAEA-CRP contr.17592, National Instruments-Costa Rica.

Evaluation of the Hydrolab HL4 water-quality sonde and sensors

USGS Publications Warehouse

Snazelle, Teri T.

2017-12-18

The U.S. Geological Survey (USGS) Hydrologic Instrumentation Facility evaluated three Hydrolab HL4 multiparameter water-quality sondes by OTT Hydromet. The sondes were equipped with temperature, conductivity, pH, dissolved oxygen (DO), and turbidity sensors. The sensors were evaluated for compliance with the USGS National Field Manual for the Collection of Water-Quality Data (NFM) criteria for continuous water-quality monitors and to verify the validity of the manufacturer’s technical specifications. The conductivity sensors were evaluated for the accuracy of the specific conductance (SC) values (conductance at 25 degrees Celsius [oC]), that were calculated by using the vendor default method, Hydrolab Fresh. The HL4’s communication protocols and operating temperature range along with accuracy of the water-quality sensors were tested in a controlled laboratory setting May 1–19, 2016. To evaluate the sonde’s performance in a surface-water field application, an HL4 equipped with temperature, conductivity, pH, DO, and turbidity sensors was deployed June 20–July 22, 2016, at USGS water-monitoring site 02492620, Pearl River at National Space Technology Laboratories (NSTL) Station, Mississippi, located near Bay Saint Louis, Mississippi, and compared to the adjacent well-maintained EXO2 site sonde.The three HL4 sondes met the USGS temperature testing criteria and the manufacturer’s technical specifications for temperature based upon the median room temperature difference between the measured and standard temperatures, but two of the three sondes exceeded the allowable difference criteria at the temperature extremes of approximately 5 and 40 ºC. Two sondes met the USGS criteria for SC. One of the sondes failed the criteria for SC when evaluated in a 100,000-microsiemens-per-centimeter (μS/cm) standard at room temperature, and also failed in a 10,000-μS/cm standard at 5, 15, and 40 ºC. All three sondes met the USGS criteria for pH and DO at room temperature

Electron Cyclotron Radiation, Related Power Loss, and Passive Current Drive in Tokamaks: A Review

DOE Office of Scientific and Technical Information (OSTI.GOV)

Fidone, Ignazio; Giruzzi, Gerardo; Granata, Giovanni

2001-01-15

A critical review on emission of weakly damped, high-harmonics electron cyclotron radiation, the related synchrotron power loss, and passive current drive in tokamaks with a fish-scale first wall is presented. First, the properties of overlapping harmonics are discussed using general analytical formulas and numerical applications. Next, the radiation power loss and efficiency of passive current drive in tokamak reactors are derived for the asymmetric fish-scale first wall. The radiation power loss is determined by the direction-averaged reflection coefficient {sigma}{sub 0} and the passive current drive by the differential reflectivity {delta}{sigma}/(1 - {sigma}{sub 0}). Finally, the problem of experimental investigations ofmore » the high harmonics radiation spectra, of {sigma}{sub 0} and {delta}{sigma}/(1 - {sigma}{sub 0}) in existing and next-step tokamaks, is discussed. Accurate measurements of the radiation spectra and the fish-scale reflectivity can be performed at arbitrary electron temperature using a partial fish-scale structure located near the tokamak equatorial plane.« less

Who will save the tokamak - Harry Potter, Arnold Schwarzenegger, or Shaquille O'Neil?

NASA Astrophysics Data System (ADS)

Freidberg, J.; Mangiarotti, F.; Minervini, J.

2014-10-01

The tokamak is the current leading contender for a fusion power reactor. The reason for the preeminence of the tokamak is its high quality plasma physics performance relative to other concepts. Even so, it is well known that the tokamak must still overcome two basic physics challenges before becoming viable as a DEMO and ultimately a reactor: (1) the achievement of non-inductive steady state operation, and (2) the achievement of robust disruption free operation. These are in addition to the PMI problems faced by all concepts. The work presented here demonstrates by means of a simple but highly credible analytic calculation that a ``standard'' tokamak cannot lead to a reactor - it is just not possible to simultaneously satisfy all the plasma physics plus engineering constraints. Three possible solutions, some more well-known than others, to the problem are analyzed. These visual image generating solutions are defined as (1) the Harry Potter solution, (2) the Arnold Schwarzenegger solution, and (3) the Shaquille O'Neil solution. Each solution will be described both qualitatively and quantitatively at the meeting.

HL7 and DICOM based integration of radiology departments with healthcare enterprise information systems.

PubMed

Blazona, Bojan; Koncar, Miroslav

2007-12-01

Integration based on open standards, in order to achieve communication and information interoperability, is one of the key aspects of modern health care information systems. However, this requirement represents one of the major challenges for the Information and Communication Technology (ICT) solutions, as systems today use diverse technologies, proprietary protocols and communication standards which are often not interoperable. One of the main producers of clinical information in healthcare settings represent Radiology Information Systems (RIS) that communicate using widely adopted DICOM (Digital Imaging and COmmunications in Medicine) standard, but in very few cases can efficiently integrate information of interest with other systems. In this context we identified HL7 standard as the world's leading medical ICT standard that is envisioned to provide the umbrella for medical data semantic interoperability, which amongst other things represents the cornerstone for the Croatia's National Integrated Healthcare Information System (IHCIS). The aim was to explore the ability to integrate and exchange RIS originated data with Hospital Information Systems based on HL7's CDA (Clinical Document Architecture) standard. We explored the ability of HL7 CDA specifications and methodology to address the need of RIS integration HL7 based healthcare information systems. We introduced the use of WADO service interconnection to IHCIS and finally CDA rendering in widely used Internet explorers. The outcome of our pilot work proves our original assumption of HL7 standard being able to adopt radiology data into the integrated healthcare systems. Uniform DICOM to CDA translation scripts and business processes within IHCIS is desired and cost effective regarding to use of supporting IHCIS services aligned to SOA.

High Adjustable Shear Map for a Single-null Divertor Tokamak

NASA Astrophysics Data System (ADS)

Whitten, Michaelangelo; Lam, Maria; Punjabi, Alkesh

1996-11-01

An explicit map that has an adjustable shear s is x1 = x0 - k y0 [ ( 1 - y0 ) ( 1 + s y0 ) + s x ^21 ] y1 = y0 + k x1 [ 1 + s ( x^21 + y^20 ) ] Tokamak shear corresponds to negative s. Thus we can construct maps for variable shear for a single-null divertor tokamak (Punjabi A, Verma A and Boozer A, Phys Rev Lett), 3322, 69 (1992) ^, (Punjabi A, Verma A and Boozer A, J Plasma Phys), 52, 91 (1994). Here we present the results from an initial study of this map. This work is supported by US DOE OFES. Michelangelo Whitten is a HU CFRT Summer Fusion High School Workshop scholar from Bowie High School in El Paso, Texas. He is supported by NASA SHARP Plus Program.

Challenges of interoperability using HL7 v3 in Czech healthcare.

PubMed

Nagy, Miroslav; Preckova, Petra; Seidl, Libor; Zvarova, Jana

2010-01-01

The paper describes several classification systems that could improve patient safety through semantic interoperability among contemporary electronic health record systems (EHR-Ss) with support of the HL7 v3 standard. We describe a proposal and a pilot implementation of a semantic interoperability platform (SIP) interconnecting current EHR-Ss by using HL7 v3 messages and concepts mappings on most widely used classification systems. The increasing number of classification systems and nomenclatures requires designing of various conversion tools for transfer between main classification systems. We present the so-called LIM filler module and the HL7 broker, which are parts of the SIP, playing the role of such conversion tools. The analysis of suitability and usability of individual terminological thesauri has been started by mapping of clinical contents of the Minimal Data Model for Cardiology (MDMC) to various terminological classification systems. A national-wide implementation of the SIP would include adopting and translating international coding systems and nomenclatures, and developing implementation guidelines facilitating the migration from national standards to international ones. Our research showed that creation of such a platform is feasible; however, it will require a huge effort to adapt fully the Czech healthcare system to the European environment.

Clinical data integration of distributed data sources using Health Level Seven (HL7) v3-RIM mapping

PubMed Central

2011-01-01

Background Health information exchange and health information integration has become one of the top priorities for healthcare systems across institutions and hospitals. Most organizations and establishments implement health information exchange and integration in order to support meaningful information retrieval among their disparate healthcare systems. The challenges that prevent efficient health information integration for heterogeneous data sources are the lack of a common standard to support mapping across distributed data sources and the numerous and diverse healthcare domains. Health Level Seven (HL7) is a standards development organization which creates standards, but is itself not the standard. They create the Reference Information Model. RIM is developed by HL7's technical committees. It is a standardized abstract representation of HL7 data across all the domains of health care. In this article, we aim to present a design and a prototype implementation of HL7 v3-RIM mapping for information integration of distributed clinical data sources. The implementation enables the user to retrieve and search information that has been integrated using HL7 v3-RIM technology from disparate health care systems. Method and results We designed and developed a prototype implementation of HL7 v3-RIM mapping function to integrate distributed clinical data sources using R-MIM classes from HL7 v3-RIM as a global view along with a collaborative centralized web-based mapping tool to tackle the evolution of both global and local schemas. Our prototype was implemented and integrated with a Clinical Database management Systems CDMS as a plug-in module. We tested the prototype system with some use case scenarios for distributed clinical data sources across several legacy CDMS. The results have been effective in improving information delivery, completing tasks that would have been otherwise difficult to accomplish, and reducing the time required to finish tasks which are used in

Impact of helical boundary conditions in MHD modeling of RFP and tokamak plasmas

NASA Astrophysics Data System (ADS)

Bonfiglio, D.; Cappello, S.; Escande, D. F.; Piovesan, P.; Veranda, M.; Chacón, L.

2012-10-01

Helical boundary conditions imposed by the active control system of the RFX-mod device provide a handle to govern the plasma dynamics in both RFP and Ohmic tokamak discharges [1]. By applying an edge radial magnetic field with proper helicity, it is possible to increase the persistence of the spontaneous helical RFP states at high current,and to stimulate them also at low current or high density. Helical BCs even allow to access helical states with different helicity than the spontaneous one [2]. In Ohmic tokamak operation at q(a)<2, the presence of the 2/1 RWM reduces the sawtoothing activity of the 1/1 internal kink, which takes a stationary snake-like character instead. Many of these features are qualitatively reproduced in 3D nonlinear MHD modeling. We study the impact of helical BCs on the MHD dynamics in both RFP and tokamak with two successfully benchmarked numerical tools, SpeCyl and PIXIE3D [3]. We recover the bifurcation from a sawtooth to a snake solution when imposing a 2/1 BC in the tokamak case and we interpret this as a toroidal/nonlinear coupling effect. We show that the bifurcation is more easily stimulated with a 1/1 BC.[4pt] [1] P. Piovesan, invited talk this meeting[0pt] [2] M. Veranda et al EPS-ICPP Conference (2012) P4.004[0pt] [3] D. Bonfiglio et al Phys. Plasmas (2010)

Scoping study for compact high-field superconducting net energy tokamaks

NASA Astrophysics Data System (ADS)

Mumgaard, R. T.; Greenwald, M.; Freidberg, J. P.; Wolfe, S. M.; Hartwig, Z. S.; Brunner, D.; Sorbom, B. N.; Whyte, D. G.

2016-10-01

The continued development and commercialization of high temperature superconductors (HTS) may enable the construction of compact, net-energy tokamaks. HTS, in contrast to present generation low temperature superconductors, offers improved performance in high magnetic fields, higher current density, stronger materials, higher temperature operation, and simplified assembly. Using HTS along with community-consensus confinement physics (H98 =1) may make it possible to achieve net-energy (Q>1) or burning plasma conditions (Q>5) in DIII-D or ASDEX-U sized, conventional aspect ratio tokamaks. It is shown that, by operating at high plasma current and density enabled by the high magnetic field (B>10T), the required triple products may be achieved at plasma volumes under 20m3, major radii under 2m, with external heating powers under 40MW. This is at the scale of existing devices operated by laboratories, universities and companies. The trade-offs in the core heating, divertor heat exhaust, sustainment, stability, and proximity to known plasma physics limits are discussed in the context of the present tokamak experience base and the requirements for future devices. The resulting HTS-based design space is compared and contrasted to previous studies on high-field copper experiments with similar missions. The physics exploration conducted with such HTS devices could decrease the real and perceived risks of ITER exploitation, and aid in quickly developing commercially-applicable tokamak pilot plants and reactors.

The Development of a Graphical User Interface Engine for the Convenient Use of the HL7 Version 2.x Interface Engine

PubMed Central

Kim, Hwa Sun; Cho, Hune

2011-01-01

Objectives The Health Level Seven Interface Engine (HL7 IE), developed by Kyungpook National University, has been employed in health information systems, however users without a background in programming have reported difficulties in using it. Therefore, we developed a graphical user interface (GUI) engine to make the use of the HL7 IE more convenient. Methods The GUI engine was directly connected with the HL7 IE to handle the HL7 version 2.x messages. Furthermore, the information exchange rules (called the mapping data), represented by a conceptual graph in the GUI engine, were transformed into program objects that were made available to the HL7 IE; the mapping data were stored as binary files for reuse. The usefulness of the GUI engine was examined through information exchange tests between an HL7 version 2.x message and a health information database system. Results Users could easily create HL7 version 2.x messages by creating a conceptual graph through the GUI engine without requiring assistance from programmers. In addition, time could be saved when creating new information exchange rules by reusing the stored mapping data. Conclusions The GUI engine was not able to incorporate information types (e.g., extensible markup language, XML) other than the HL7 version 2.x messages and the database, because it was designed exclusively for the HL7 IE protocol. However, in future work, by including additional parsers to manage XML-based information such as Continuity of Care Documents (CCD) and Continuity of Care Records (CCR), we plan to ensure that the GUI engine will be more widely accessible for the health field. PMID:22259723

The Development of a Graphical User Interface Engine for the Convenient Use of the HL7 Version 2.x Interface Engine.

PubMed

Kim, Hwa Sun; Cho, Hune; Lee, In Keun

2011-12-01

The Health Level Seven Interface Engine (HL7 IE), developed by Kyungpook National University, has been employed in health information systems, however users without a background in programming have reported difficulties in using it. Therefore, we developed a graphical user interface (GUI) engine to make the use of the HL7 IE more convenient. The GUI engine was directly connected with the HL7 IE to handle the HL7 version 2.x messages. Furthermore, the information exchange rules (called the mapping data), represented by a conceptual graph in the GUI engine, were transformed into program objects that were made available to the HL7 IE; the mapping data were stored as binary files for reuse. The usefulness of the GUI engine was examined through information exchange tests between an HL7 version 2.x message and a health information database system. Users could easily create HL7 version 2.x messages by creating a conceptual graph through the GUI engine without requiring assistance from programmers. In addition, time could be saved when creating new information exchange rules by reusing the stored mapping data. The GUI engine was not able to incorporate information types (e.g., extensible markup language, XML) other than the HL7 version 2.x messages and the database, because it was designed exclusively for the HL7 IE protocol. However, in future work, by including additional parsers to manage XML-based information such as Continuity of Care Documents (CCD) and Continuity of Care Records (CCR), we plan to ensure that the GUI engine will be more widely accessible for the health field.

Effects of Resonant Helical Field on Toroidal Field Ripple in IR-T1 Tokamak

NASA Astrophysics Data System (ADS)

Mahdavipour, B.; Salar Elahi, A.; Ghoranneviss, M.

2018-02-01

The toroidal magnetic field which is created by toroidal coils has the ripple in torus space. This magnetic field ripple has an importance in plasma equilibrium and stability studies in tokamak. In this paper, we present the investigation of the interaction between the toroidal magnetic field ripple and resonant helical field (RHF). We have estimated the amplitude of toroidal field ripples without and with RHF (with different q = m/n) ( m = 2, m = 3, m = 4, m = 5, m = 2 & 3, n = 1) using “Comsol Multiphysics” software. The simulations show that RHF has effects on the toroidal ripples.

New botanical drug, HL tablet, reduces hepatic fat as measured by magnetic resonance spectroscopy in patients with nonalcoholic fatty liver disease: A placebo-controlled, randomized, phase II trial

PubMed Central

Jeong, Jae Yoon; Sohn, Joo Hyun; Baek, Yang Hyun; Cho, Yong Kyun; Kim, Yongsoo; Kim, Hyeonjin

2017-01-01

AIM To evaluate the efficacy and safety of HL tablet extracted from magnolia officinalis for treating patients with nonalcoholic fatty liver disease (NAFLD). METHODS Seventy-four patients with NAFLD diagnosed by ultrasonography were randomly assigned to 3 groups given high dose (400 mg) HL tablet, low dose (133.4 mg) HL tablet and placebo, respectively, daily for 12 wk. The primary endpoint was post-treatment change of hepatic fat content (HFC) measured by magnetic resonance spectroscopy. Secondary endpoints included changes of serum aspartate aminotransferase, alanine aminotransferase (ALT), cholesterol, triglyceride, free fatty acid, homeostasis model assessment-estimated insulin resistance, and body mass index (BMI). RESULTS The mean HFC of the high dose HL group, but not of the low dose group, declined significantly after 12 wk of treatment (high dose vs placebo, P = 0.033; low dose vs placebo, P = 0.386). The mean changes of HFC from baseline at week 12 were -1.7% ± 3.1% in the high dose group (P = 0.018), -1.21% ± 4.97% in the low dose group (P = 0.254) and 0.61% ± 3.87% in the placebo group (relative changes compared to baseline, high dose were: -12.1% ± 23.5%, low dose: -3.2% ± 32.0%, and placebo: 7.6% ± 44.0%). Serum ALT levels also tended to decrease in the groups receiving HL tablet while other factors were unaffected. There were no moderate or severe treatment-related safety issues during the study. CONCLUSION HL tablet is effective in reducing HFC without any negative lipid profiles, BMI changes and adverse effects. PMID:28932090

Kinematics and the origin of the internal structures in HL Tauri jet (HH 151)

NASA Astrophysics Data System (ADS)

Movsessian, T. A.; Magakian, T. Yu.; Moiseev, A. V.

2012-05-01

Context. Knotty structures of Herbig-Haro jets are common phenomena, and knowing the origin of these structures is essential for understanding the processes of jet formation. Basically, there are two theoretical approaches: different types of instabilities in stationary flow, and velocity variations in the flow. Aims: We investigate the structures with different radial velocities in the knots of the HL Tau jet as well as its unusual behaviour starting from 20'' from the source. Collation of radial velocity data with proper motion measurements of emission structures in the jet of HL Tau makes it possible to understand the origin of these structures and decide on the mechanism for the formation of the knotty structures in Herbig-Haro flows. Methods: We present observations obtained with a 6 m telescope (Russia) using the SCORPIO camera with scanning Fabry-Perót interferometer. Two epochs of the observations of the HL/XZ Tau region in Hα emission (2001 and 2007) allowed us to measure proper motions for high and low radial velocity structures. Results: The structures with low and high radial velocities in the HL Tau jet show the same proper motion. The point where the HL Tau jet bents to the north (it coincides with the trailing edge of so-called knot A) is stationary, i.e. does not have any perceptible proper motion and is visible in Hα emission only. Conclusions: We conclude that the high- and low-velocity structures in the HL Tau jet represent bow-shocks and Mach disks in the internal working surfaces of episodic outflows. The bend of the jet and the brightness increase starting some distance from the source coincides with the observed stationary deflecting shock. The increase of relative surface brightness of bow-shocks could be the result of the abrupt change of the physical conditions of the ambient medium as well as the interaction of a highly collimated flow and the side wind from XZ Tau. Based on observations collected with the 6 m telescope of the Special

Taraxinic acid, a hydrolysate of sesquiterpene lactone glycoside from the Taraxacum coreanum NAKAI, induces the differentiation of human acute promyelocytic leukemia HL-60 cells.

PubMed

Choi, Jung-Hye; Shin, Kyung-Min; Kim, Na-Young; Hong, Jung-Pyo; Lee, Yong Sup; Kim, Hyoung Ja; Park, Hee-Juhn; Lee, Kyung-Tae

2002-11-01

The present work was performed to elucidate the active moiety of a sesquiterpene lactone, taraxinic acid-1'-O-beta-D-glucopyranoside (1). from Taraxacum coreanum NAKAI on the cytotoxicity of various cancer cells. Based on enzymatic hydrolysis and MTT assay, the active moiety should be attributed to the aglycone taraxinic acid (1a). rather than the glycoside (1). Taraxinic acid exhibited potent antiproliferative activity against human leukemia-derived HL-60. In addition, this compound was found to be a potent inducer of HL-60 cell differentiation as assessed by a nitroblue tetrazolium reduction test, esterase activity assay, phagocytic activity assay, morphology change, and expression of CD 14 and CD 66 b surface antigens. These results suggest that taraxinic acid induces the differentiation of human leukemia cells to monocyte/macrophage lineage. Moreover, the expression level of c-myc was down-regulated during taraxinic acid-dependent HL-60 cell differentiation, whereas p21(CIP1) and p27(KIP1) were up-regulated. Taken together, our results suggest that taraxinic acid may have potential as a therapeutic agent in human leukemia.

An HL7-CDA wrapper for facilitating semantic interoperability to rule-based Clinical Decision Support Systems.

PubMed

Sáez, Carlos; Bresó, Adrián; Vicente, Javier; Robles, Montserrat; García-Gómez, Juan Miguel

2013-03-01

The success of Clinical Decision Support Systems (CDSS) greatly depends on its capability of being integrated in Health Information Systems (HIS). Several proposals have been published up to date to permit CDSS gathering patient data from HIS. Some base the CDSS data input on the HL7 reference model, however, they are tailored to specific CDSS or clinical guidelines technologies, or do not focus on standardizing the CDSS resultant knowledge. We propose a solution for facilitating semantic interoperability to rule-based CDSS focusing on standardized input and output documents conforming an HL7-CDA wrapper. We define the HL7-CDA restrictions in a HL7-CDA implementation guide. Patient data and rule inference results are mapped respectively to and from the CDSS by means of a binding method based on an XML binding file. As an independent clinical document, the results of a CDSS can present clinical and legal validity. The proposed solution is being applied in a CDSS for providing patient-specific recommendations for the care management of outpatients with diabetes mellitus. Copyright © 2012 Elsevier Ireland Ltd. All rights reserved.

Precision searches in dijets at the HL-LHC and HE-LHC

NASA Astrophysics Data System (ADS)

Chekanov, S. V.; Childers, J. T.; Proudfoot, J.; Wang, R.; Frizzell, D.

2018-05-01

This paper explores the physics reach of the High-Luminosity Large Hadron Collider (HL-LHC) for searches of new particles decaying to two jets. We discuss inclusive searches in dijets and b-jets, as well as searches in semi-inclusive events by requiring an additional lepton that increases sensitivity to different aspects of the underlying processes. We discuss the expected exclusion limits for generic models predicting new massive particles that result in resonant structures in the dijet mass. Prospects of the Higher-Energy LHC (HE-LHC) collider are also discussed. The study is based on the Pythia8 Monte Carlo generator using representative event statistics for the HL-LHC and HE-LHC running conditions. The event samples were created using supercomputers at NERSC.

Realizing Steady State Tokamak Operation for Fusion Energy

NASA Astrophysics Data System (ADS)

Luce, T. C.

2009-11-01

Continuous operation of a tokamak for fusion energy has obvious engineering advantages, but also presents physics challenges beyond the achievement of conditions needed for a burning plasma. The power from fusion reactions and external sources must support both the pressure and the current equilibrium without inductive current drive, leading to demands on stability, confinement, current drive, and plasma-wall interactions that exceed those for pulsed tokamaks. These conditions have been met individually in the present generation of tokamaks, and significant progress has been made in the last decade to realize scenarios where the required conditions are obtained simultaneously. Tokamaks are now operated routinely without disruptions close to the ideal MHD pressure limit, as needed for steady-state operation. Scenarios that project to high fusion gain have been demonstrated where more than half of the current is supplied by the ``bootstrap'' current generated by the pressure gradient in the plasma. Fully noninductive sustainment has been obtained for about a resistive time (the longest intrinsic time scale in the confined plasma) with normalized pressure and confinement approaching those needed for demonstration of steady-state conditions in ITER. One key challenge remaining to be addressed is how to handle the demanding heat and particle fluxes expected in a steady-state tokamak without compromising the high level of core plasma performance. Rather than attempt a comprehensive historical survey, this review will start from the plasma requirements of a steady-state tokamak powerplant, illustrate with examples the progress made in both experimental and theoretical understanding, and point to the remaining physics challenges.

Far-infrared laser diagnostics on the HT-6M tokamak

NASA Astrophysics Data System (ADS)

Gao, X.; Lu, H. J.; Guo, Q. L.; Wan, Y. X.; Tong, X. D.

1995-01-01

A multichannel far-infrared (FIR) hydrogen cyanide (HCN) laser interferometer was developed to measure plasma electron density profile on the HT-6M tokamak. The structure of the seven-channel FIR laser interferometer is described. The laser source used in the interferometer was a continuous-wave glow discharge HCN laser with a cavity length of 3.4 m and power output of about 100 mW at 337 μm. The detection sensitivity was 1/15 fringe with a temporal resolution of 0.1 ms. Experimental results were measured by the seven-channel FIR HCN laser interferometer with edge Ohmic heating, a pumping limiter, and ion cyclotron resonant heating on the HT-6M tokamak are reported.

Control effectiveness and tip-fin dihedral effects for the HL-20 lifting-body configuration at Mach numbers from 1.6 to 4.5

NASA Technical Reports Server (NTRS)

Cruz, Christopher I.; Ware, George M.

1995-01-01

Wind tunnel tests were made with a scale model of the HL-20 in the Langley Unitary Plan Wind Tunnel. Pitch control was investigated by deflecting the elevon surfaces on the outboard fins and body flaps on the fuselage. Yaw control tests were made with the all movable center fin deflected 5 deg. Almost full negative body flap deflection (-30 deg) was required to trim the HL-20 (moment reference center at 0.54-percent body length from nose) to positive values of life in the Mach number range from 1.6 to 2.5. Elevons were twice as effective as body flaps as a longitudinal trim device. The elevons were effective as a roll control, but because of tip-fin dihedral angle, produced about as much adverse yawing moment as rolling moment. The body flaps were less effective in producing rolling moment, but produced little adverse yawing moment. The yaw effectiveness of the all movable center fin was essentially constant over the angle-of-attack range at each Mach number. The value of yawing moment, however, was small. Center-fin deflection produced almost no rolling moments. The model was directionally unstable over most of the Mach number range with tip-fin dihedral angles less than the baseline value of 50 deg.

Semantic interoperability--HL7 Version 3 compared to advanced architecture standards.

PubMed

Blobel, B G M E; Engel, K; Pharow, P

2006-01-01

To meet the challenge for high quality and efficient care, highly specialized and distributed healthcare establishments have to communicate and co-operate in a semantically interoperable way. Information and communication technology must be open, flexible, scalable, knowledge-based and service-oriented as well as secure and safe. For enabling semantic interoperability, a unified process for defining and implementing the architecture, i.e. structure and functions of the cooperating systems' components, as well as the approach for knowledge representation, i.e. the used information and its interpretation, algorithms, etc. have to be defined in a harmonized way. Deploying the Generic Component Model, systems and their components, underlying concepts and applied constraints must be formally modeled, strictly separating platform-independent from platform-specific models. As HL7 Version 3 claims to represent the most successful standard for semantic interoperability, HL7 has been analyzed regarding the requirements for model-driven, service-oriented design of semantic interoperable information systems, thereby moving from a communication to an architecture paradigm. The approach is compared with advanced architectural approaches for information systems such as OMG's CORBA 3 or EHR systems such as GEHR/openEHR and CEN EN 13606 Electronic Health Record Communication. HL7 Version 3 is maturing towards an architectural approach for semantic interoperability. Despite current differences, there is a close collaboration between the teams involved guaranteeing a convergence between competing approaches.

Realizing steady-state tokamak operation for fusion energy

NASA Astrophysics Data System (ADS)

Luce, T. C.

2011-03-01

Continuous operation of a tokamak for fusion energy has clear engineering advantages but requires conditions beyond those sufficient for a burning plasma. The fusion reactions and external sources must support both the pressure and the current equilibrium without inductive current drive, leading to demands on stability, confinement, current drive, and plasma-wall interactions that exceed those for pulsed tokamaks. These conditions have been met individually, and significant progress has been made in the past decade to realize scenarios where the required conditions are obtained simultaneously. Tokamaks are operated routinely without disruptions near pressure limits, as needed for steady-state operation. Fully noninductive sustainment with more than half of the current from intrinsic currents has been obtained for a resistive time with normalized pressure and confinement approaching those needed for steady-state conditions. One remaining challenge is handling the heat and particle fluxes expected in a steady-state tokamak without compromising the core plasma performance.

Tokamaks

DOE Office of Scientific and Technical Information (OSTI.GOV)

Wesson, J

1987-01-01

The word tokamak derives from the Russian term, toroidalnaya kamera magnitaya (toroidal chamber magnetic). The device was invented in the Soviet Union in 1950 and has since developed into one of the chief ways in which it is hoped to obtain usable power from plasmas through thermonuclear fusion. The present is meant to be an introduction to those entering the field, to those already engaged in research, and to those who want to gain some understanding of what it's all about.

E3 Success Story - Rising Above the Rest: H&L Advantage

EPA Pesticide Factsheets

Through the Green Suppliers Network review process, H&L Advantage worked on site with Michigan's Department of Environmental Quality (DEQ) and The Right Place, to identify over a dozen improvement opportunities in August 2004.

High power heating of magnetic reconnection in merging tokamak experiments

DOE Office of Scientific and Technical Information (OSTI.GOV)

Ono, Y.; Tanabe, H.; Gi, K.

2015-05-15

Significant ion/electron heating of magnetic reconnection up to 1.2 keV was documented in two spherical tokamak plasma merging experiment on MAST with the significantly large Reynolds number R∼10{sup 5}. Measured 1D/2D contours of ion and electron temperatures reveal clearly energy-conversion mechanisms of magnetic reconnection: huge outflow heating of ions in the downstream and localized heating of electrons at the X-point. Ions are accelerated up to the order of poloidal Alfven speed in the reconnection outflow region and are thermalized by fast shock-like density pileups formed in the downstreams, in agreement with recent solar satellite observations and PIC simulation results. The magneticmore » reconnection efficiently converts the reconnecting (poloidal) magnetic energy mostly into ion thermal energy through the outflow, causing the reconnection heating energy proportional to square of the reconnecting (poloidal) magnetic field B{sub rec}{sup 2}  ∼  B{sub p}{sup 2}. The guide toroidal field B{sub t} does not affect the bulk heating of ions and electrons, probably because the reconnection/outflow speeds are determined mostly by the external driven inflow by the help of another fast reconnection mechanism: intermittent sheet ejection. The localized electron heating at the X-point increases sharply with the guide toroidal field B{sub t}, probably because the toroidal field increases electron confinement and acceleration length along the X-line. 2D measurements of magnetic field and temperatures in the TS-3 tokamak merging experiment also reveal the detailed reconnection heating mechanisms mentioned above. The high-power heating of tokamak merging is useful not only for laboratory study of reconnection but also for economical startup and heating of tokamak plasmas. The MAST/TS-3 tokamak merging with B{sub p} > 0.4 T will enables us to heat the plasma to the alpha heating regime: T{sub i} > 5 keV without using any additional heating

Beta-mangostin from Cratoxylum arborescens activates the intrinsic apoptosis pathway through reactive oxygen species with downregulation of the HSP70 gene in the HL60 cells associated with a G0/G1 cell-cycle arrest.

PubMed

Omer, Fatima Abdelmutaal Ahmed; Hashim, Najihah Binti Mohd; Ibrahim, Mohamed Yousif; Dehghan, Firouzeh; Yahayu, Maizatulakmal; Karimian, Hamed; Salim, Landa Zeenelabdin Ali; Mohan, Syam

2017-11-01

Xanthones are phytochemical compounds found in a number of fruits and vegetables. Characteristically, they are noted to be made of diverse properties based on their biological, biochemical, and pharmacological actions. Accordingly, the apoptosis mechanisms induced by beta-mangostin, a xanthone compound isolated from Cratoxylum arborescens in the human promyelocytic leukemia cell line (HL60) in vitro, were examined in this study. The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay was done to estimate the cytotoxicity effect of β-mangostin on the HL60 cell line. Acridine orange/propidium iodide and Hoechst 33342 dyes and Annexin V tests were conducted to detect the apoptosis features. Caspase-3 and caspase-9 activities; reactive oxygen species; real-time polymerase chain reaction for Bcl-2, Bax, caspase-3, and caspase-9 Hsp70 genes; and western blot for p53, cytochrome c, and pro- and cleavage-caspase-3 and caspase-9 were assessed to examine the apoptosis mechanism. Cell-cycle analysis conducted revealed that β-mangostin inhibited the growth of HL60 at 58 µM in 24 h. The administration of β-mangostin with HL60 caused cell morphological changes related to apoptosis which increased the number of early and late apoptotic cells. The β-mangostin-catalyzed apoptosis action through caspase-3, caspase-7, and caspase-9 activation overproduced reactive oxygen species which downregulated the expression of antiapoptotic genes Bcl-2 and HSP70. Conversely, the expression of the apoptotic genes Bax, caspase-3, and caspase-9 were upregulated. Meanwhile, at the protein level, β-mangostin activated the formation of cleaved caspase-3 and caspase-9 and also upregulated the p53. β-mangostin arrested the cell cycle at the G 0 /G 1 phase. Overall, the results for β-mangostin showed an antiproliferative effect in HL60 via stopping the cell cycle at the G 0 /G 1 phase and prompted the intrinsic apoptosis pathway.

Utilization of the human cell line HL-60 for chemiluminescence based detection of microorganisms and related substances.

PubMed

Timm, Michael; Hansen, Erik W; Moesby, Lise; Christensen, Jens D

2006-02-01

In this paper we describe a new pyrogen assay using the human leukemia cell line HL-60. The cell line is differentiated using all-trans retinoic acid (ATRA) to generate a cell population that resembles mature granulocytes. The differentiated HL-60 cell is capable of generating reactive oxygen species (ROS) when challenged with pyrogenic substances. In a luminol enhanced chemilumimetric assay the responsiveness of differentiated HL-60 cells is tested towards Salmonella typhimurium, Bacillus subtilis, Saccharomyces cerevisiae, Candida albicans, lipopolysaccharide (LPS) and lipoteichoic acid (LTA). The results show a poor sensitivity to S. typhimurium but displays good sensitivity towards B. subtilis, LTA and LPS. Furthermore, the sensitivity towards the yeasts C. albicans and S. cerevisiae is considerably better than obtained in other in vitro cell systems. Overall these results indicate that the HL-60 cell assay possibly could be evolved to a supplementary assay for the known pyrogenic detection assays. Furthermore, the utilization of the assay for pyrogenic examination of recombinant drugs derived from yeast expression systems would be relevant to examine.

BIGEL analysis of gene expression in HL60 cells exposed to X rays or 60 Hz magnetic fields

NASA Technical Reports Server (NTRS)

Balcer-Kubiczek, E. K.; Zhang, X. F.; Han, L. H.; Harrison, G. H.; Davis, C. C.; Zhou, X. J.; Ioffe, V.; McCready, W. A.; Abraham, J. M.; Meltzer, S. J.

1998-01-01

We screened a panel of 1,920 randomly selected cDNAs to discover genes that are differentially expressed in HL60 cells exposed to 60 Hz magnetic fields (2 mT) or X rays (5 Gy) compared to unexposed cells. Identification of these clones was accomplished using our two-gel cDNA library screening method (BIGEL). Eighteen cDNAs differentially expressed in X-irradiated compared to control HL60 cells were recovered from a panel of 1,920 clones. Differential expression in experimental compared to control cells was confirmed independently by Northern blotting of paired total RNA samples hybridized to each of the 18 clone-specific cDNA probes. DNA sequencing revealed that 15 of the 18 cDNA clones produced matches with the database for genes related to cell growth, protein synthesis, energy metabolism, oxidative stress or apoptosis (including MYC, neuroleukin, copper zinc-dependent superoxide dismutase, TC4 RAS-like protein, peptide elongation factor 1alpha, BNIP3, GATA3, NF45, cytochrome c oxidase II and triosephosphate isomerase mRNAs). In contrast, BIGEL analysis of the same 1,920 cDNAs revealed no differences greater than 1.5-fold in expression levels in magnetic-field compared to sham-exposed cells. Magnetic-field-exposed and control samples were analyzed further for the presence of mRNA encoding X-ray-responsive genes by hybridization of the 18 specific cDNA probes to RNA from exposed and control HL60 cells. Our results suggest that differential gene expression is induced in approximately 1% of a random pool of cDNAs by ionizing radiation but not by 60 Hz magnetic fields under the present experimental conditions.

Edge-localized-modes in tokamaks

DOE PAGES

Leonard, Anthony W.

2014-09-11

Edge-localized-modes (ELMs) are a ubiquitous feature of H-mode in tokamaks. When gradients in the H-mode transport barrier grow to exceed the MHD stability limit the ELM instability grows explosively rapidly transporting energy and particles onto open field lines and material surfaces. Though ELMs provide additional particle and impurity transport through the H-mode transport barrier, enabling steady operation, the resulting heat flux transients to plasma facing surfaces project to large amplitude in future low collisionality burning plasma tokamaks. Measurements of the ELM heat flux deposition onto material surfaces in the divertor and main chamber indicate significant broadening compared to inter-ELM heatmore » flux, with a timescale for energy deposition that is consistent with sonic ion flow and numerical simulation. Comprehensive ELM simulation is highlighting the important physics processes of ELM transport including parallel transport due to magnetic reconnection and turbulence resulting from collapse of the H-mode transport barrier. As a result, encouraging prospects for ELM control and/or suppression in future tokamaks include intrinsic modes of ELM free operation, ELM triggering with frequent small pellet injection and the application of 3D magnetic fields.« less

Antiproliferative activity of pristimerin isolated from Maytenus ilicifolia (Celastraceae) in human HL-60 cells.

PubMed

Costa, Patricia Marçal da; Ferreira, Paulo Michel Pinheiro; Bolzani, Vanderlan da Silva; Furlan, Maysa; de Freitas Formenton Macedo Dos Santos, Vânia Aparecida; Corsino, Joaquim; de Moraes, Manoel Odorico; Costa-Lotufo, Letícia Veras; Montenegro, Raquel Carvalho; Pessoa, Cláudia

2008-06-01

Pristimerin has been shown to be cytotoxic to several cancer cell lines. In the present work, the cytotoxicity of pristimerin was evaluated in human tumor cell lines and in human peripheral blood mononuclear cells (PBMC). This work also examined the effects of pristimerin (0.4; 0.8 and 1.7 microM) in HL-60 cells, after 6, 12 and 24h of exposure. Pristimerin reduced the number of viable cells and increased number of non-viable cells in a concentration-dependent manner by tripan blue test showing morphological changes consistent with apoptosis. Nevertheless, pristimerin was not selective to cancer cells, since it inhibited PBMC proliferation with an IC50 of 0.88 microM. DNA synthesis inhibition assessed by 5-bromo-2'-deoxyuridine (BrdU) incorporation in HL-60 cells was 70% and 83% for the concentrations of 0.4 and 0.8 microM, respectively. Pristimerin (10 and 20 microM) was not able to inhibit topoisomerase I. In AO/EB (acridine orange/ethidium bromide) staining, all tested concentrations reduced the number of HL-60 viable cells, with the occurrence of necrosis and apoptosis in a concentration-dependent manner, results in agreement with trypan blue exclusion findings. The analysis of membrane integrity and internucleosomal DNA fragmentation by flow cytometry in the presence of pristimerin indicated that treated cells underwent apoptosis. The present data point to the importance of pristimerin as representative of an emerging class of potential anticancer chemicals, exhibiting an antiproliferative effect by inhibiting DNA synthesis and triggering apoptosis.

The Spherical Tokamak MEDUSA for Costa Rica

NASA Astrophysics Data System (ADS)

Ribeiro, Celso; Vargas, Ivan; Guadamuz, Saul; Mora, Jaime; Ansejo, Jose; Zamora, Esteban; Herrera, Julio; Chaves, Esteban; Romero, Carlos

2012-10-01

The former spherical tokamak (ST) MEDUSA (Madison EDUcation Small Aspect.ratio tokamak, R<0.14m, a<0.10m, BT<0.5T, Ip<40kA, 3ms pulse)[1] is in a process of donation to Costa Rica Institute of Technology. The main objective of MEDUSA is to train students in plasma physics /technical related issues which will help all tasks of the very low aspect ratio stellarator SCR-1(A≡R/>=3.6, under design[2]) and also the ongoing activities in low temperature plasmas. Courses in plasma physics at undergraduate and post-graduate joint programme levels are regularly conducted. The scientific programme is intend to clarify several issues in relevant physics for conventional and mainly STs, including transport, heating and current drive via Alfv'en wave, and natural divertor STs with ergodic magnetic limiter[3,4]. [1] G.D.Garstka, PhD thesis, University of Wisconsin at Madison, 1997 [2] L.Barillas et al., Proc. 19^th Int. Conf. Nucl. Eng., Japan, 2011 [3] C.Ribeiro et al., IEEJ Trans. Electrical and Electronic Eng., 2012(accepted) [4] C.Ribeiro et al., Proc. 39^th EPS Conf. Contr. Fusion and Plasma Phys., Sweden, 2012

Advances in Dust Detection and Removal for Tokamaks

NASA Astrophysics Data System (ADS)

Campos, A.; Skinner, C. H.; Roquemore, A. L.; Leisure, J. O. V.; Wagner, S.

2008-11-01

Dust diagnostics and removal techniques are vital for the safe operation of next step fusion devices such as ITER. An electrostatic dust detector[1] developed in the laboratory is being applied to NSTX. In the tokamak environment, large particles or fibres can fall on the grid potentially causing a permanent short. We report on the development of a gas puff system that uses helium to clear such particles from the detector. Experiments with varying nozzle designs, backing pressures, puff durations, and exit flow orientations have obtained an optimal configuration that effectively removes particles from a 25 cm^2 area. Dust removal from next step tokamaks will be required to meet regulatory dust limits. A tripolar grid of fine interdigitated traces has been designed that generates an electrostatic travelling wave for conveying dust particles to a ``drain.'' First trials have shown particle motion in optical microscope images. [1] C. H. Skinner et al., J. Nucl. Mater., 376 (2008) 29.

A polysaccharide from the fruiting bodies of Agaricus blazei Murill induces caspase-dependent apoptosis in human leukemia HL-60 cells.

PubMed

Li, Xiaohui; Zhao, Xin; Wang, Hongmin; Han, Junqing; Liu, Li

2014-09-01

Polysaccharides are the major active ingredients of fungus Agaricus blazei for treating and preventing cancer. However, there are no reports showing anti-tumor activity of A. blazei polysaccharides (ABP) on human leukemia (HL)-60 cells in vitro and in vivo. In this study, we demonstrated that ABP efficiently inhibited proliferation of cultured HL-60 cells, which was associated with the induction of apoptosis. The increase in ABP-induced apoptosis was accompanied by loss of mitochondria membrane potential (∆Ψm), cytochrome c release from the mitochondria, activation of caspase-3, degradation of poly(ADP-ribose) polymerase (PARP), and the elevated ratio of Bcl-2-associated X (Bax)/B-cell lymphoma 2 (Bcl-2). Moreover, z-DEVD-fmk, a caspase-3 inhibitor, reversed the cytotoxic effects and apoptotic characteristics induced by ABP in HL-60 cells. Furthermore, we confirmed that ABP could obviously inhibit the solid cancer growth of leukemia HL-60 in tumor xenograft model. These data demonstrated that ABP effectively induced the apoptosis of HL-60 cells via a signaling cascade of mitochondrial caspase-3-dependent pathway.

Electronics for CMS Endcap Muon Level-1 Trigger System Phase-1 and HL LHC upgrades

NASA Astrophysics Data System (ADS)

Madorsky, A.

2017-07-01

To accommodate high-luminosity LHC operation at a 13 TeV collision energy, the CMS Endcap Muon Level-1 Trigger system had to be significantly modified. To provide robust track reconstruction, the trigger system must now import all available trigger primitives generated by the Cathode Strip Chambers and by certain other subsystems, such as Resistive Plate Chambers (RPC). In addition to massive input bandwidth, this also required significant increase in logic and memory resources. To satisfy these requirements, a new Sector Processor unit has been designed. It consists of three modules. The Core Logic module houses the large FPGA that contains the track-finding logic and multi-gigabit serial links for data exchange. The Optical module contains optical receivers and transmitters; it communicates with the Core Logic module via a custom backplane section. The Pt Lookup table (PTLUT) module contains 1 GB of low-latency memory that is used to assign the final Pt to reconstructed muon tracks. The μ TCA architecture (adopted by CMS) was used for this design. The talk presents the details of the hardware and firmware design of the production system based on Xilinx Virtex-7 FPGA family. The next round of LHC and CMS upgrades starts in 2019, followed by a major High-Luminosity (HL) LHC upgrade starting in 2024. In the course of these upgrades, new Gas Electron Multiplier (GEM) detectors and more RPC chambers will be added to the Endcap Muon system. In order to keep up with all these changes, a new Advanced Processor unit is being designed. This device will be based on Xilinx UltraScale+ FPGAs. It will be able to accommodate up to 100 serial links with bit rates of up to 25 Gb/s, and provide up to 2.5 times more logic resources than the device used currently. The amount of PTLUT memory will be significantly increased to provide more flexibility for the Pt assignment algorithm. The talk presents preliminary details of the hardware design program.

Compact fusion energy based on the spherical tokamak

NASA Astrophysics Data System (ADS)

Sykes, A.; Costley, A. E.; Windsor, C. G.; Asunta, O.; Brittles, G.; Buxton, P.; Chuyanov, V.; Connor, J. W.; Gryaznevich, M. P.; Huang, B.; Hugill, J.; Kukushkin, A.; Kingham, D.; Langtry, A. V.; McNamara, S.; Morgan, J. G.; Noonan, P.; Ross, J. S. H.; Shevchenko, V.; Slade, R.; Smith, G.

2018-01-01

Tokamak Energy Ltd, UK, is developing spherical tokamaks using high temperature superconductor magnets as a possible route to fusion power using relatively small devices. We present an overview of the development programme including details of the enabling technologies, the key modelling methods and results, and the remaining challenges on the path to compact fusion.

Polysaccharopeptides derived from Coriolus versicolor potentiate the S-phase specific cytotoxicity of Camptothecin (CPT) on human leukemia HL-60 cells.

PubMed

Wan, Jennifer Man-Fan; Sit, Wai-Hung; Yang, Xiaotong; Jiang, Pingping; Wong, Leo Lap-Yan

2010-04-27

Polysaccharopeptide (PSP) from Coriolus versicolor (Yunzhi) is used as a supplementary cancer treatment in Asia. The present study aims to investigate whether PSP pre-treatment can increase the response of the human leukemia HL-60 cells to apoptosis induction by Camptothecin (CPT). We used bivariate bromodeoxyuridine/propidium iodide (BrdUrd/PI) flow cytometry analysis to measure the relative movement (RM) of the BrdUrd positively labeled cells and DNA synthesis time (Ts) on the HL-60 cell line. We used annexin V/PI flow cytometry analysis to quantify the viable, necrotic and apoptotic cells. The expression of cyclin E and cyclin B1 was determined with annexin V/PI flow cytometry and western blotting. Human peripheral blood mononuclear cells were used to test the cytotoxicity of PSP and CPT. PSP reduced cellular proliferation; inhibited cells progression through both S and G2 phase, reduced 3H-thymidine uptake and prolonged DNA synthesis time (Ts) in HL-60 cells. PSP-pretreated cells enhanced the cytotoxicity of CPT. The sensitivity of cells to the cytotoxic effects of CPT was seen to be the highest in the S-phase and to a small extent of the G2 phase of the cell cycle. On the other hand, no cell death (measured by annexin V/PI) was evident with the normal human peripheral blood mononuclear cells with treatment of either PSP or CPT. The present study shows that PSP increases the sensitization of the HL-60 cells to undergo effective apoptotic cell death induced by CPT. The pattern of sensitivity of cancer cells is similar to that of HL-60 cells. PSP rapidly arrests and/or kills cells in S-phase and did not interfere with the anticancer action of CPT. PSP is a potential adjuvant to treat human leukemia as rapidly proliferating tumors is characterized by a high proportion of S-phase cells.

Stationary Flowing Liquid Lithium (SFLiLi) systems for tokamaks

NASA Astrophysics Data System (ADS)

Zakharov, Leonid; Gentile, Charles; Roquemore, Lane

2013-10-01

The present approach to magnetic fusion which relies on high recycling plasma-wall interaction has exhausted itself at the level of TFTR, JET, JT-60 devices with no realistic path to the burning plasma. Instead, magnetic fusion needs a return to its original idea of insulation of the plasma from the wall, which was the dominant approach in the 1970s and upon implementations has a clear path to the DEMO device with PDT ~= 100 MW and Qelectric > 1 . The SFLiLi systems of this talk is the technology tool for implementation of the guiding idea of magnetic fusion. It utilizes the unique properties of flowing LiLi to pump plasma particles and, thus, insulate plasma from the walls. The necessary flow rate, ~= 1 g3/s, is very small, thus, making the use of lithium practical and consistent with safety requirements. The talk describes how chemical activity of LiLi, which is the major technology challenge of using LiLi in tokamaks, is addressed by SFLiLi systems at the level of already performed (HT-7) experiment, and in ongoing implementations for a prototype of SFLiLi for tokamak divertors and the mid-plane limiter for EAST tokamak (to be tested in the next experimental campaign). This work is supported by US DoE contract No. DE-AC02-09-CH11466.

[Isolation and identification of berberine from endophytic fungi HL-Y-3].

PubMed

Zhang, Feng-Hua; Xiang, Jian-Hui; Cui, Wen-Xia; Yu, Jiang; Wang, Yan; Li, Qin-Fan

2016-08-01

The endophytic fungi HL-Y-3, which was isolated from the healthy leaves of Coptis chinensis, produced berberine when grown in the PDA culture medium. The presence of berberine was confirmed by the chromatographic and spectroscopic analyses. The yield of berberine was recorded as 9.313 μg•g⁻¹ by HPLC. The strain HL-Y-3 was identified as Alternaria sp.by morphological observation and 5.8S rDNA-ITS sequence analysis.The separation and purification of constituents were performed by PTLC. The mass spectrometry (MS) of the analyte was shown to be identical with authentic berberine.Further analysis with nuclear magnetic resonance (NMR) spectroscopy to showed that the chemical structure of the fungal berberine was identical with authentic berberine. The research provided new resources for the utilization of berberine. Copyright© by the Chinese Pharmaceutical Association.

Differentiation-inducing effects of small fruit juices on HL-60 leukemic cells.

PubMed

Yoshizawa, Y; Kawaii, S; Urashima, M; Fukase, T; Sato, T; Murofushi, N; Nishimura, H

2000-08-01

Epidemiological studies indicate that high intakes of fruits and vegetables are associated with a reduced risk of cancer, and several plant-derived drugs have been developed in medical oncology. Since only a small part of the flora has been tested for any kind of bioactivity, we chose small fruits as sources of differentiation-inducing activity against HL-60 leukemic cells. We have prepared juices from various small fruits that grow mainly in the northern part of Japan. Screening of 43 samples indicated that juices of Actinidia polygama Maxim., Rosa rugosa Thunb., Vaccinium smallii A. Gray, and Sorbus sambucifolia Roem. strongly induced differentiation of HL-60 cells to monocyte/macrophage characteristics in a concentration-dependent manner as indicated by histochemical and biochemical examinations.

Tokamak und Stellarator - zwei Wege zur Fusionsenergie: Fusionsforschung

NASA Astrophysics Data System (ADS)

Milch, Isabella

2006-07-01

Im Laufe der Fusionsforschung haben sich zwei Bautypen für ein zukünftiges Kraftwerk als besonders aussichtsreich erwiesen: Tokamak und Stellarator. Mit dem geplanten Tokamak-Experimentalreaktor ITER steht die internationale Fusionsforschung vor der Demonstration eines Energie liefernden Plasmas. Parallel soll die in Greifswald entstehende Forschungsanlage Wendelstein 7-X die Kraftwerkstauglichkeit des alternativen Bauprinzips der Stellaratoren zeigen.

Regulated expression of the MRP8 and MRP14 genes during terminal differentiation of human promyelocytic leukemic HL-60 cells

DOE Office of Scientific and Technical Information (OSTI.GOV)

Warner-Bartnicki, A.L.; Murao, S.; Collart, F.R.

1992-02-14

The calcium-binding proteins MRP8 and MRP14 are induced during monomyelocytic cell maturation and may mediate the growth arrest in differentiating HL-60 cells. We determined the levels of a protein complex (PC) containing MRP8 and MRP14 and investigated the mechanism by which the genes encoding these proteins are regulated in HL-60 cells treated with the differentiation-inducing agent mycophenolic acid. Elevated levels of the PC were found to directly parallel gains in the steady-state levels of MRP8 and MRP14 mRNA. Transcription studies with the use of nuclear run-on experiments revealed increased transcription initiation at the MRP8 and MRP14 promoters after MPA treatment.more » 1{alpha},25-Dihydroxyvitamin D{sub 3}, which induces HL-60 cell differentiation by another mechanism, was also found to increase transcription initiation at the MRP8 and MRP14 promoters, suggesting that this initiation is the major control of MRP8 and MRP14 gene expression during terminal differentiation of human promyelocytic cells.« less

Development of DEMO-FNS tokamak for fusion and hybrid technologies

NASA Astrophysics Data System (ADS)

Kuteev, B. V.; Azizov, E. A.; Alexeev, P. N.; Ignatiev, V. V.; Subbotin, S. A.; Tsibulskiy, V. F.

2015-07-01

The history of fusion-fission hybrid systems based on a tokamak device as an extremely efficient DT-fusion neutron source has passed through several periods of ample research activity in the world since the very beginning of fusion research in the 1950s. Recently, a new roadmap of the hybrid program has been proposed with the goal to build a pilot hybrid plant (PHP) in Russia by 2030. Development of the DEMO-FNS tokamak for fusion and hybrid technologies, which is planned to be built by 2023, is the key milestone on the path to the PHP. This facility is in the phase of conceptual design aimed at providing feasibility studies for a full set of steady state tokamak technologies at a fusion energy gain factor Q ˜ 1, fusion power of ˜40 MW and opportunities for testing a wide range of hybrid technologies with the emphasis on continuous nuclide processing in molten salts. This paper describes the project motivations, its current status and the key issues of the design.

Upgrade plans for the ATLAS Forward Calorimeter at the HL-LHC

NASA Astrophysics Data System (ADS)

Rutherfoord, John; ATLAS Liquid Argon Calorimeter Group

2012-12-01

Although data-taking at CERN's Large Hadron Collider (LHC) is expected to continue for a number of years, plans are already being developed for operation of the LHC and associated detectors at an increased instantaneous luminosity about 5 times the original design value of 1034 cm-2 s-1. The increased particle flux at this high luminosity (HL) will have an impact on many sub-systems of the ATLAS detector. In particular, in the liquid argon forward calorimeter (FCal), which was designed for operation at LHC luminosities, the associated increase in the ionization load at HL-LHC luminosities creates a number of problems which can degrade its performance. These include space-charge effects in the liquid argon gaps, excessive drop in potential across the gaps due to large HV supply currents through the protection resistors, and an increase in temperature which may cause the liquid argon to boil. One solution, which would require opening both End-Cap cryostats, is the construction and installation of new FCals with narrower liquid argon gaps, lowering the values of the protection resistors, and the addition of cooling loops. A second proposed solution, which does not require opening the cryostat cold volume, is the addition of a small, warm calorimeter in front of each existing FCal, resulting in a reduction of the particle flux to levels at which the existing FCal can operate normally.

Electron cyclotron emission from nonthermal tokamak plasmas

DOE Office of Scientific and Technical Information (OSTI.GOV)

Harvey, R.W.; O'Brien, M.R.; Rozhdestvensky, V.V.

1993-02-01

Electron cyclotron emission can be a sensitive indicator of nonthermal electron distributions. A new, comprehensive ray-tracing and cyclotron emission code that is aimed at predicting and interpreting the cyclotron emission from tokamak plasmas is described. The radiation transfer equation is solved along Wentzel--Kramers--Brillouin (WKB) rays using a fully relativistic calculation of the emission and absorption from electron distributions that are gyrotropic and toroidally symmetric, but may be otherwise arbitrary functions of the constants of motion. Using a radial array of electron distributions obtained from a bounce-averaged Fokker--Planck code modeling dc electron field and electron cyclotron heating effects, the cyclotron emissionmore » spectra are obtained. A pronounced strong nonthermal cyclotron emission feature that occurs at frequencies relativistically downshifted to second harmonic cyclotron frequencies outside the tokamak is calculated, in agreement with experimental results from the DIII-D [J. L. Luxon and L. G. Davies, Fusion Technol. [bold 8], 441 (1985)] and FT-1 [D. G. Bulyginsky [ital et] [ital al]., in [ital Proceedings] [ital of] [ital the] 15[ital th] [ital European] [ital Conference] [ital on] [ital Controlled] [ital Fusion] [ital and] [ital Plasma] [ital Heating], Dubrovnik, 1988 (European Physical Society, Petit-Lancy, 1988), Vol. 12B, Part II, p. 823] tokamaks. The calculations indicate the presence of a strong loss mechanism that operates on electrons in the 100--150 keV energy range.« less

The Aneutronic Rodless Ultra Low Aspect Ratio Tokamak

NASA Astrophysics Data System (ADS)

Ribeiro, Celso

2016-10-01

The replacement of the metal centre-post in spherical tokamaks (STs) by a plasma centre-post (PCP, the TF current carrier) is the ideal scenario for a ST reactor. A simple rodless ultra low aspect-ratio tokamak (RULART) using a screw-pinch PCP ECR-assisted with an external solenoid has been proposed in the most compact RULART [Ribeiro C, SOFE-15]. There the solenoid provided the stabilizing field for the PCP and the toroidal electrical field for the tokamak start-up, which will stabilize further the PCP, acting as stabilizing closed conducting surface. Relative low TF will be required. The compactness (high ratio of plasma-spherical vessel volume) may provide passive stabilization and easier access to L-H mode transition. It is presented here: 1) stability analysis of the PCP (initially MHD stable due to the hollow J profile); 2) tokamak equilibrium simulations, and 3) potential use for aneutronic reactions studies via pairs of proton p and boron 11B ion beams in He plasmas. The beams' line-of-sights sufficiently miss the sources of each other, thus allowing a near maximum relative velocities and reactivity. The reactions should occur close to the PCP mid-plane. Some born alphas should cross the PCP and be dragged by the ion flow (higher momentum exchange) towards the anode but escape directly to a direct electricity converter. Others will reach evenly the vessel directly or via thermal diffusion (favourable heating by the large excursion 2a), leading to the lowest power wall load possible. This might be a potential hybrid direct-steam cycle conversion reactor scheme, nearly aneutronic, and with no ash or particle retention problems, as opposed to the D-T thermal reaction proposals.

Final technical report for DE-SC00012633 AToM (Advanced Tokamak Modeling)

DOE Office of Scientific and Technical Information (OSTI.GOV)

Holland, Christopher; Orlov, Dmitri; Izzo, Valerie

This final report for the AToM project documents contributions from University of California, San Diego researchers over the period of 9/1/2014 – 8/31/2017. The primary focus of these efforts was on performing validation studies of core tokamak transport models using the OMFIT framework, including development of OMFIT workflow scripts. Additional work was performed to develop tools for use of the nonlinear magnetohydrodynamics code NIMROD in OMFIT, and its use in the study of runaway electron dynamics in tokamak disruptions.

Cytotoxic and apoptotic effects of different extracts of Artemisia biennis Willd. on K562 and HL-60 cell lines

PubMed Central

Tayarani-Najaran, Zahra; Makki, Farideh-Sadat; Alamolhodaei, Nafiseh-Sadat; Mojarrab, Mahdi; Emami, Seyed Ahmad

2017-01-01

Objective(s): Artemisia is a genus of herbs and small shrubs forms an important part of natural vegetation in Iran. It has been reported that several Artemisia species possess anti-proliferative effects. Considering the value of this genus in anti-cancer researches we have chosen Artemisia biennis for cytotoxic and mechanistic studies. Materials and Methods: In this study we have investigated the cytotoxic and apoptotic effects of petroleum ether, dichloromethane, ethyl acetate, ethanol, and ethanol: water (1:1 v/v) extracts of A. biennis Willd. on two cancer human cell lines (K562 and HL-60) and J774 as normal cells. Results: CH2Cl2 extract was found to have the highest anti-proliferative effect on cancer cells. IC50 values obtained in AlamarBlue® assay for CH2Cl2 extract were 64.86 and 54.31 µg/ml on K562 and HL-60 cells respectively. In flow cytometry histogram of the cells treated with CH2Cl2 extract, sub-G1 peak was induced. DNA fragmentation, increased in the level of Bax and cleavage of PARP protein all showed the induction of apoptosis with CH2Cl2 extract after 48 hr contact with cells. Conclusion: The results can corroborate the cytotoxic and apoptotic effects of the CH2Cl2 extract of A. biennis on the K562 and HL-60 cancer cell lines. PMID:28293393

Potent apoptosis-inducing activity of erypoegin K, an isoflavone isolated from Erythrina poeppigiana, against human leukemia HL-60 cells.

PubMed

Hikita, Kiyomi; Hattori, Natsuki; Takeda, Aya; Yamakage, Yuko; Shibata, Rina; Yamada, Saori; Kato, Kuniki; Murata, Tomiyasu; Tanaka, Hitoshi; Kaneda, Norio

2018-01-01

Erypoegin K is an isoflavone isolated from the stem bark of Erythrina poeppigiana. It contains a furan group at the A-ring of the core isoflavone structure and can inhibit the activity of glyoxalase I, an enzyme that catalyzes the detoxification of methylglyoxal (MG), a by-product of glycolysis. In the present study, we found that erypoegin K has a potent cytotoxic effect on human leukemia HL-60 cells. Its cytotoxic effect was much stronger than that of a known glyoxalase I inhibitor S-p-bromobenzylglutathione cyclopentyl diester. Conversely, erypoegin K demonstrated weak cytotoxicity toward normal human peripheral lymphocytes. The treatment of HL-60 cells with erypoegin K significantly induced caspase-3 activity, whereas the pretreatment of the cells with caspase-3 inhibitor suppressed erypoegin K-induced cell death. Furthermore, nuclear condensation and apoptotic genome DNA fragmentation were observed in erypoegin K-treated HL-60 cells. These results indicated that the observed cell death was mediated by apoptosis. In addition, the toxic compound MG was highly accumulated in the culture medium of erypoegin K-treated HL-60 cells, suggesting that cell apoptosis was triggered by extracellular MG. The present study showed that erypoegin K has a potent apoptosis-inducing effect on cancerous cell lines, such as HL-60.

Frontline brentuximab vedotin in combination with dacarbazine or bendamustine in patients aged ≥60 years with HL.

PubMed

Friedberg, Jonathan W; Forero-Torres, Andres; Bordoni, Rodolfo E; Cline, Vivian J M; Patel Donnelly, Dipti; Flynn, Patrick J; Olsen, Gregg; Chen, Robert; Fong, Abraham; Wang, Yinghui; Yasenchak, Christopher A

2017-12-28

Patients aged ≥60 years with treatment-naive Hodgkin lymphoma (HL) have few treatment options and inferior survival due to treatment-related toxicities and comorbidities. This phase 2, nonrandomized, open-label study evaluated brentuximab vedotin (BV) monotherapy (results previously reported), BV plus dacarbazine (DTIC), and BV plus bendamustine. Patients had classical HL and were ineligible for or declined frontline chemotherapy. Twenty-two patients received 1.8 mg/kg BV and 375 mg/m 2 DTIC for up to 12 cycles, and 20 more patients received 1.8 mg/kg BV plus 90 or 70 mg/m 2 bendamustine for up to 6 cycles (dose reduced due to toxicity). Subsequent BV monotherapy was allowed. Approximately 30 patients were to receive BV plus bendamustine; however, the incidence of serious adverse events (65%) and 2 deaths on study led to discontinuation of bendamustine and cessation of enrollment. Most patients had stage III/IV disease, and approximately half had ≥3 comorbidities or were impaired in ≥1 aspect that significantly interfered with quality of life. For BV plus DTIC, the objective response rate (ORR) was 100% and the complete remission (CR) rate was 62%. To date, the median progression-free survival (PFS) is 17.9 months. For BV plus bendamustine, the ORR was 100% and the CR rate was 88%. Neither the median PFS nor overall survival was reached. For elderly patients with HL, BV plus DTIC may be a frontline option based on tolerability and response duration. Despite activity, BV plus bendamustine is not a tolerable regimen in these patients. This trial was registered at www.clinicaltrials.gov as #NCT01716806. © 2017 by The American Society of Hematology.

HlSRB, a Class B Scavenger Receptor, Is Key to the Granulocyte-Mediated Microbial Phagocytosis in Ticks

PubMed Central

Aung, Kyaw Min; Boldbaatar, Damdinsuren; Umemiya-Shirafuji, Rika; Liao, Min; Tsuji, Naotoshi; Xuenan, Xuan; Suzuki, Hiroshi; Kume, Aiko; Galay, Remil Linggatong; Tanaka, Tetsuya; Fujisaki, Kozo

2012-01-01

Ixodid ticks transmit various pathogens of deadly diseases to humans and animals. However, the specific molecule that functions in the recognition and control of pathogens inside ticks is not yet to be identified. Class B scavenger receptor CD36 (SRB) participates in internalization of apoptotic cells, certain bacterial and fungal pathogens, and modified low-density lipoproteins. Recently, we have reported on recombinant HlSRB, a 50-kDa protein with one hydrophobic SRB domain from the hard tick, Haemaphysalis longicornis. Here, we show that HlSRB plays vital roles in granulocyte-mediated phagocytosis to invading Escherichia coli and contributes to the first-line host defense against various pathogens. Data clearly revealed that granulocytes that up-regulated the expression of cell surface HlSRB are almost exclusively involved in hemocyte-mediated phagocytosis for E. coli in ticks, and post-transcriptional silencing of the HlSRB-specific gene ablated the granulocytes' ability to phagocytose E. coli and resulted in the mortality of ticks due to high bacteremia. This is the first report demonstrating that a scavenger receptor molecule contributes to hemocyte-mediated phagocytosis against exogenous pathogens, isolated and characterized from hematophagous arthropods. PMID:22479406

Constrained ripple optimization of Tokamak bundle divertors

DOE Office of Scientific and Technical Information (OSTI.GOV)

Hively, L.M.; Rome, J.A.; Lynch, V.E.

1983-02-01

Magnetic field ripple from a tokamak bundle divertor is localized to a small toroidal sector and must be treated differently from the usual (distributed) toroidal field (TF) coil ripple. Generally, in a tokamak with an unoptimized divertor design, all of the banana-trapped fast ions are quickly lost due to banana drift diffusion or to trapping between the 1/R variation in absolute value vector B ..xi.. B and local field maxima due to the divertor. A computer code has been written to optimize automatically on-axis ripple subject to these constraints, while varying up to nine design parameters. Optimum configurations have lowmore » on-axis ripple (<0.2%) so that, now, most banana-trapped fast ions are confined. Only those ions with banana tips near the outside region (absolute value theta < or equal to 45/sup 0/) are lost. However, because finite-sized TF coils have not been used in this study, the flux bundle is not expanded.« less

Edge Thomson scattering diagnostic on COMPASS tokamak: Installation, calibration, operation, improvements

DOE Office of Scientific and Technical Information (OSTI.GOV)

Bohm, P., E-mail: bohm@ipp.cas.cz; Bilkova, P.; Melich, R.

2014-11-15

The core Thomson scattering diagnostic (TS) on the COMPASS tokamak was put in operation and reported earlier. Implementation of edge TS, with spatial resolution along the laser beam up to ∼1/100 of the tokamak minor radius, is presented now. The procedure for spatial calibration and alignment of both core and edge systems is described. Several further upgrades of the TS system, like a triggering unit and piezo motor driven vacuum window shutter, are introduced as well. The edge TS system, together with the core TS, is now in routine operation and provides electron temperature and density profiles.

Quality control of FWC during assembly and commissioning in SST-1 Tokamak

NASA Astrophysics Data System (ADS)

Patel, Hitesh; Santra, Prosenjit; Parekh, Tejas; Biswas, Prabal; Jayswal, Snehal; Chauhan, Pradeep; Paravastu, Yuvakiran; George, Siju; Semwal, Pratibha; Thankey, Prashant; Ramesh, Gattu; Prakash, Arun; Dhanani, Kalpesh; Raval, D. C.; Khan, Ziauddin; Pradhan, Subrata

2017-04-01

First Wall Components (FWC) of SST-1 tokamak, which are in the immediate vicinity of plasma, comprises of limiters, divertors, baffles, passive stabilizers designed to operate long duration (∼1000 s) discharges of elongated plasma. All FWC consist of copper alloy heat sink modules with SS cooling tubes brazed onto it, graphite tiles acting as armour material facing the plasma, and are mounted to the vacuum vessels with suitable Inconel support structures at inter-connected ring & port locations. The FWC are very recently assembled and commissioned successfully inside the vacuum vessel of SST-1 undergoing a rigorous quality control and checks at every stage of the assembly process. This paper will present the quality control aspects and checks of FWC from commencement of assembly procedure, namely material test reports, leak testing of high temperature baked components, assembled dimensional tolerances, leak testing of all welded joints, graphite tile tightening torques, electrical continuity and electrical isolation of passive stabilizers from vacuum vessel, baking and cooling hydraulic connections inside vacuum vessel.

Saturated internal instabilities in advanced-tokamak plasmas

NASA Astrophysics Data System (ADS)

Hua, M.-D.; Chapman, I. T.; Pinches, S. D.; Hastie, R. J.; MAST Team

2010-06-01

"Advanced tokamak" (AT) scenarios were developed with the aim of reaching steady-state operation in future potential tokamak fusion power plants. AT scenarios exhibit non-monotonic to flat safety factor profiles (q, a measure of the magnetic field line pitch), with the minimum q (qmin) slightly above an integer value (qs). However, it has been predicted that these q profiles are unstable to ideal magnetohydrodynamic instabilities as qmin approaches qs. These ideal instabilities, observed and diagnosed as such for the first time in MAST plasmas with AT-like q profiles, have far-reaching consequences like confinement degradation, flattening of the toroidal core rotation or enhanced fast ion losses. These observations motivate the stability analysis of advanced-tokamak plasmas, with a view to provide guidance for stability thresholds in AT scenarios. Additionally, the measured rotation damping is compared to the self-consistently calculated predictions from neoclassical toroidal viscosity theory.

The cali meteorite fell: A new H/L ordinary chondrite

USGS Publications Warehouse

Rodriguez, J.M.T.; Llorca, J.; Rubin, A.E.; Grossman, J.N.; Sears, D.W.G.; Naranjo, M.; Bretzius, S.; Tapia, M.; Sepulveda, M.H.G.

2009-01-01

The fall of the Cali meteorite took place on 6 July 2007 at 16 h 32 ?? 1 min local time (21 h 32 ?? 1 min UTC). A daylight fireball was witnessed by hundreds of people in the Cauca Valley in Colombia from which 10 meteorite samples with a total mass of 478 g were recovered near 3??24.3'N, 76??30.6'W. The fireball trajectory and radiant have been reconstructed with moderate accuracy. From the computed radiant and from considering various plausible velocities, we obtained a range of orbital solutions that suggest that the Cali progenitor meteoroid probably originated in the main asteroid belt. Based on petrography, mineral chemistry, magnetic susceptibility, fhermoluminescence, and bulk chemistry, the Cali meteorite is classified as an H/L4 ordinary chondrite breccia.

Six-degree-of-freedom guidance and control-entry analysis of the HL-20

NASA Technical Reports Server (NTRS)

Powell, Richard W.

1993-01-01

The ability of the HL-20 lifting body to fly has been evaluated for an automated entry from atmospheric interface to landing. This evaluation was required to demonstrate that not only successful touchdown conditions would be possible for this low lift-to-drag-ratio vehicle, but also the vehicle would not exceed its design dynamic pressure limit of 400 psf during entry. This dynamic pressure constraint limit, coupled with limited available pitch-control authority at low supersonic speeds, restricts the available maneuvering capability for the HL-20 to acquire the runway. One result of this analysis was that this restrictive maneuvering capability does not allow the use of a model-following atmospheric entry-guidance algorithm, such as that used by the Space Shuttle, but instead requires a more adaptable guidance algorithm. Therefore, for this analysis, a predictor-corrector guidance algorithm was developed that would provide successful touchdown conditions while not violating the dynamic pressure constraint. A flight-control system was designed and incorporated, along with the predictor-corrector guidance algorithm, into a six-DOF simulation. which showed that the HL-20 remained controllable and could reach the landing site and execute a successful landing under all off-nominal conditions simulated.

Magnetic Frequency Response of HL-LHC Beam Screens

DOE Office of Scientific and Technical Information (OSTI.GOV)

Morrone, M.; Martino, M.; De Maria, R.

Magnetic fields used to control particle beams in accelerators are usually controlled by regulating the electrical current of the power converters. In order to minimize lifetime degradation and ultimately luminosity loss in circular colliders, current-noise is a highly critical figure of merit of power converters, in particular for magnets located in areas with high beta-function, like the High Luminosity Large Hadron Collider (HL-LHC) insertions. However, what is directly acting upon the beam is the magnetic field and not the current of the power converter, which undergoes several frequency-dependent transformations until the desired magnetic field, seen by the beam, is obtained.more » Beam screens are very rarely considered when assessing or specifying the noise figure of merit, but their magnetic frequency response is such that they realize relatively effective low pass filtering of the magnetic field produced by the system magnet-power converter. This work aims at filling this gap by quantifying the expected impact of different beam screen layouts for the most relevant HL-LHC insertion magnets. A welldefined post-processing technique is used to derive the frequency response of the different multipoles from multi-physics Finite Element Method (FEM) simulation results. In addition, a well approximated analytical formula for the low-frequency range of multi-layered beam screens is presented.« less

Evaluation and Comparison of High-Resolution (HR) and High-Light (HL) Phosphors in the Micro-Angiographic Fluoroscope (MAF) using Generalized Linear Systems Analyses (GMTF, GDQE) that include the Effect of Scatter, Magnification and Detector Characteristics.

PubMed

Gupta, Sandesh K; Jain, Amit; Bednarek, Daniel R; Rudin, Stephen

2011-01-01

In this study, we evaluated the imaging characteristics of the high-resolution, high-sensitivity micro-angiographic fluoroscope (MAF) with 35-micron pixel-pitch when used with different commercially-available 300 micron thick phosphors: the high resolution (HR) and high light (HL) from Hamamatsu. The purpose of this evaluation was to see if the HL phosphor with its higher screen efficiency could be replaced with the HR phosphor to achieve improved resolution without an increase in noise resulting from the HR's decreased light-photon yield. We designated the detectors MAF-HR and MAF-HL and compared them with a standard flat panel detector (FPD) (194 micron pixel pitch and 600 micron thick CsI(Tl)). For this comparison, we used the generalized linear-system metrics of GMTF, GNNPS and GDQE which are more realistic measures of total system performance since they include the effect of scattered radiation, focal spot distribution, and geometric un-sharpness. Magnifications (1.05-1.15) and scatter fractions (0.28 and 0.33) characteristic of a standard head phantom were used. The MAF-HR performed significantly better than the MAF-HL at high spatial frequencies. The ratio of GMTF and GDQE of the MAF-HR compared to the MAF-HL at 3(6) cycles/mm was 1.45(2.42) and 1.23(2.89), respectively. Despite significant degradation by inclusion of scatter and object magnification, both MAF-HR and MAF-HL provide superior performance over the FPD at higher spatial frequencies with similar performance up to the FPD's Nyquist frequency of 2.5 cycles/mm. Both substantially higher resolution and improved GDQE can be achieved with the MAF using the HR phosphor instead of the HL phosphor.

Comparisons between tokamak fueling of gas puffing and supersonic molecular beam injection in 2D simulations

DOE PAGES

Zhou, Y. L.; Wang, Z. H.; Xu, X. Q.; ...

2015-01-09

Plasma fueling with high efficiency and deep injection is very important to enable fusion power performance requirements. It is a powerful and efficient way to study neutral transport dynamics and find methods of improving the fueling performance by doing large scale simulations. Furthermore, two basic fueling methods, gas puffing (GP) and supersonic molecular beam injection (SMBI), are simulated and compared in realistic divertor geometry of the HL-2A tokamak with a newly developed module, named trans-neut, within the framework of BOUT++ boundary plasma turbulence code [Z. H. Wang et al., Nucl. Fusion 54, 043019 (2014)]. The physical model includes plasma density,more » heat and momentum transport equations along with neutral density, and momentum transport equations. In transport dynamics and profile evolutions of both plasma and neutrals are simulated and compared between GP and SMBI in both poloidal and radial directions, which are quite different from one and the other. It finds that the neutrals can penetrate about four centimeters inside the last closed (magnetic) flux surface during SMBI, while they are all deposited outside of the LCF during GP. Moreover, it is the radial convection and larger inflowing flux which lead to the deeper penetration depth of SMBI and higher fueling efficiency compared to GP.« less

Comparisons between tokamak fueling of gas puffing and supersonic molecular beam injection in 2D simulations

DOE Office of Scientific and Technical Information (OSTI.GOV)

Zhou, Y. L.; Wang, Z. H.; Xu, X. Q.

Plasma fueling with high efficiency and deep injection is very important to enable fusion power performance requirements. It is a powerful and efficient way to study neutral transport dynamics and find methods of improving the fueling performance by doing large scale simulations. Furthermore, two basic fueling methods, gas puffing (GP) and supersonic molecular beam injection (SMBI), are simulated and compared in realistic divertor geometry of the HL-2A tokamak with a newly developed module, named trans-neut, within the framework of BOUT++ boundary plasma turbulence code [Z. H. Wang et al., Nucl. Fusion 54, 043019 (2014)]. The physical model includes plasma density,more » heat and momentum transport equations along with neutral density, and momentum transport equations. In transport dynamics and profile evolutions of both plasma and neutrals are simulated and compared between GP and SMBI in both poloidal and radial directions, which are quite different from one and the other. It finds that the neutrals can penetrate about four centimeters inside the last closed (magnetic) flux surface during SMBI, while they are all deposited outside of the LCF during GP. Moreover, it is the radial convection and larger inflowing flux which lead to the deeper penetration depth of SMBI and higher fueling efficiency compared to GP.« less

Comparisons between tokamak fueling of gas puffing and supersonic molecular beam injection in 2D simulations

DOE Office of Scientific and Technical Information (OSTI.GOV)

Zhou, Y. L.; Southwestern Institute of Physics, Chengdu 610041; Wang, Z. H., E-mail: zhwang@swip.ac.cn

Plasma fueling with high efficiency and deep injection is very important to enable fusion power performance requirements. It is a powerful and efficient way to study neutral transport dynamics and find methods of improving the fueling performance by doing large scale simulations. Two basic fueling methods, gas puffing (GP) and supersonic molecular beam injection (SMBI), are simulated and compared in realistic divertor geometry of the HL-2A tokamak with a newly developed module, named trans-neut, within the framework of BOUT++ boundary plasma turbulence code [Z. H. Wang et al., Nucl. Fusion 54, 043019 (2014)]. The physical model includes plasma density, heatmore » and momentum transport equations along with neutral density, and momentum transport equations. Transport dynamics and profile evolutions of both plasma and neutrals are simulated and compared between GP and SMBI in both poloidal and radial directions, which are quite different from one and the other. It finds that the neutrals can penetrate about four centimeters inside the last closed (magnetic) flux surface during SMBI, while they are all deposited outside of the LCF during GP. It is the radial convection and larger inflowing flux which lead to the deeper penetration depth of SMBI and higher fueling efficiency compared to GP.« less

Implementations of the HL7 Context-Aware Knowledge Retrieval (“Infobutton”) Standard: Challenges, Strengths, Limitations, and Uptake

PubMed Central

Del Fiol, Guilherme; Huser, Vojtech; Strasberg, Howard R; Maviglia, Saverio M; Curtis, Clayton; Cimino, James J

2012-01-01

To support clinical decision-making,computerized information retrieval tools known as “infobuttons” deliver contextually-relevant knowledge resources intoclinical information systems.The Health Level Seven International(HL7)Context-Aware Knowledge Retrieval (Infobutton) Standard specifies a standard mechanism to enable infobuttons on a large scale. Objective To examine the experience of organizations in the course of implementing the HL7 Infobutton Standard. Method Cross-sectionalonline survey and in-depth phone interviews. Results A total of 17 organizations participated in the study.Analysis of the in-depth interviews revealed 20 recurrent themes.Implementers underscored the benefits, simplicity, and flexibility of the HL7 Infobutton Standard. Yet, participants voiced the need for easier access to standard specifications and improved guidance to beginners. Implementers predicted that the Infobutton Standard will be widely or at least fairly well adopted in the next five years, but uptake will dependlargely on adoption among electronic health record (EHR) vendors. To accelerate EHR adoption of the Infobutton Standard,implementers recommended HL7-compliant infobutton capabilities to be included in the United States Meaningful Use Certification Criteria EHR systems. Limitations Opinions and predictions should be interpreted with caution, since all the participant organizations have successfully implemented the Standard and overhalf of the organizations were actively engaged in the development of the Standard. Conclusion Overall, implementers reported a very positive experience with the HL7 Infobutton Standard.Despite indications of increasing uptake, measures should be taken to stimulate adoption of the Infobutton Standard among EHR vendors. Widespread adoption of the Infobutton standard has the potential to bring contextually relevant clinical decision support content into the healthcare provider workflow. PMID:22226933

Stability at high performance in the MAST spherical tokamak

NASA Astrophysics Data System (ADS)

Buttery, R. J.; Akers, R.; Arends, E.; Conway, N. J.; Counsell, G. F.; Cunningham, G.; Gimblett, C. G.; Gryaznevich, M.; Hastie, R. J.; Hole, M. J.; Lehane, I.; Martin, R.; Patel, A.; Pinfold, T.; Sauter, O.; Taylor, D.; Turri, G.; Valovic, M.; Walsh, M. J.; Wilson, H. R.; MAST Team

2004-09-01

The development of reliable H-modes on MAST, together with advances in heating power and a range of high spatial resolution diagnostics, has provided a platform to enable MAST to address some of the most important issues of tokamak stability. In particular the high bgr potential of the spherical tokamak is highlighted with stable operation at bgrN ~ 5-6, bgrT ~ 16% and bgrp up to ~2. Magnetic diagnostic evaluation of the global bgr parameters is independently confirmed by kinetic profile data. Calculations indicate that the bgrN values are in the vicinity of no-wall stability limits. Studies of neoclassical tearing modes (NTMs) have been extended to explore their effects and develop avoidance strategies. Experiments have demonstrated that sawteeth play a strong role in triggering NTMs—by avoiding large sawteeth a much higher bgrN value has been reached. The significance of NTMs is confirmed, with large islands observed using the 300 point Thomson scattering diagnostic, and locking of large n = 1 modes frequently leading to disruptions, which become more rapid at low q95. The role of error fields has been explored. H-mode plasmas are also limited by edge localized modes (ELMs), with confinement degraded as the ELM frequency rises. However, in contrast to the conventional tokamak, the ELMs in high performing regimes on MAST (HIPB98Y2 ~ 1) appear to be type III in nature. Modelling using the ELITE code, which incorporates finite n corrections, identifies instability to peeling modes, consistent with a type III interpretation. It also shows considerable scope to raise pressure gradients before ballooning type modes (perhaps associated with type I ELMs) occur. The calculations show that narrow pedestals can support much stronger pressure gradients than might be expected from simple n = infin ballooning calculations. Finally sawteeth are shown to degrade confinement by ~10-15% in particular cases examined. They are observed not to remove the q = 1 surface in the cases

Scan Rate Dependent Spin Crossover Iron(II) Complex with Two Different Relaxations and Thermal Hysteresis fac-[Fe(II)(HL(n-Pr))3]Cl·PF6 (HL(n-Pr) = 2-Methylimidazol-4-yl-methylideneamino-n-propyl).

PubMed

Fujinami, Takeshi; Nishi, Koshiro; Hamada, Daisuke; Murakami, Keishiro; Matsumoto, Naohide; Iijima, Seiichiro; Kojima, Masaaki; Sunatsuki, Yukinari

2015-08-03

Solvent-free spin crossover Fe(II) complex fac-[Fe(II)(HL(n-Pr))3]Cl·PF6 was prepared, where HL(n-Pr) denotes 2-methylimidazol-4-yl-methylideneamino-n-propyl. The magnetic susceptibility measurements at scan rate of 0.5 K min(-1) showed two successive spin transition processes consisting of the first spin transition T1 centered at 122 K (T1↑ = 127.1 K, T1↓ = 115.8 K) and the second spin transition T2 centered at ca. 105 K (T2↑ = 115.8 K, T2↓ = 97.2 K). The magnetic susceptibility measurements at the scan rate of 2.0, 1.0, 0.5, 0.25, and 0.1 K min(-1) showed two scan speed dependent spin transitions, while the Mössbauer spectra detected only the first spin transition T1. The crystal structures were determined at 160, 143, 120, 110, 95 K in the cooling mode, and 110, 120, and 130 K in the warming mode so as to follow the spin transition process of high-spin HS → HS(T1) → HS(T2) → low-spin LS → LS(T2) → LS(T1) → HS. The crystal structures at all temperatures have a triclinic space group P1̅ with Z = 2. The complex-cation has an octahedral N6 coordination geometry with three bidentate ligands and assume a facial-isomer with Δ- and Λ-enantimorphs. Three imidazole groups of fac-[Fe(II)(HL(n-Pr))3](2+) are hydrogen-bonded to three Cl(-) ions. The 3:3 NH(imidazole)···Cl(-) hydrogen-bonds form a stepwise ladder assembly structure, which is maintained during the spin transition process. The spin transition process is related to the structural changes of the FeN6 coordination environment, the order-disorder of PF6(-) anion, and the conformation change of n-propyl groups. The Fe-N bond distance in the HS state is longer by 0.2 Å than that in the LS state. Disorder of PF6(-) anion is not observed in the LS state but in the HS state. The conformational changes of n-propyl groups are found in the spin transition processes except for HS → HS(T1) → HS(T2).

Optimization study of normal conductor tokamak for commercial neutron source

NASA Astrophysics Data System (ADS)

Fujita, T.; Sakai, R.; Okamoto, A.

2017-05-01

The optimum conceptual design of tokamak with normal conductor coils was studied for minimizing the cost for producing a given neutron flux by using a system code, PEC. It is assumed that the fusion neutrons are used for burning transuranics from the fission reactor spent fuel in the blanket and a fraction of the generated electric power is circulated to opearate the tokamak with moderate plasma fusion gain. The plasma performance was assumed to be moderate ones; {β\\text{N}}~∼ ~3{--}4 in the aspect ratio A~=~2{--}3 and {{H}98y2}~=~1 . The circulating power is an important factor affecting the cost. Though decreasing the aspect ratio is useful to raise the plasma beta and decrease the toroidal field, the maximum field in the coil starts to rise in the very low aspect ratio range and then the circulating power increases with decrease in the plasma aspect ratio A below A~∼ ~2 , while the construction cost increases with A . As a result, the cost per neutron has its minimum around A~∼ ~2.2 , namely, between ST and the conventional tokamak. The average circulating power fraction is expected to be ~51%.

Plasma density injection and flow during coaxial helicity injection in a tokamak

NASA Astrophysics Data System (ADS)

Hooper, E. B.

2018-02-01

Whole device, resistive MHD simulations of spheromaks and tokamaks have used a large diffusion coefficient that maintains a nearly constant density throughout the device. In the present work, helicity and plasma are coinjected into a low-density plasma in a tokamak with a small diffusion coefficient. As in previous simulations [Hooper et al., Phys. Plasmas 20, 092510 (2013)], a flux bubble is formed, which expands to fill the tokamak volume. The injected plasma is non-uniform inside the bubble. The flow pattern is analyzed; when the simulation is not axisymmetric, an n = 1 mode on the surface of the bubble generates leakage of plasma into the low-density volume. Closed flux is generated following injection, as in experiments and previous simulations. The result provides a more detailed physics analysis of the injection, including density non-uniformities in the plasma that may affect its use as a startup plasma [Raman et al., Phys. Rev. Lett. 97, 175002 (2006)].

Using OPC and HL7 Standards to Incorporate an Industrial Big Data Historian in a Health IT Environment.

PubMed

Cruz, Márcio Freire; Cavalcante, Carlos Arthur Mattos Teixeira; Sá Barretto, Sérgio Torres

2018-05-30

Health Level Seven (HL7) is one of the standards most used to centralize data from different vital sign monitoring systems. This solution significantly limits the data available for historical analysis, because it typically uses databases that are not effective in storing large volumes of data. In industry, a specific Big Data Historian, known as a Process Information Management System (PIMS), solves this problem. This work proposes the same solution to overcome the restriction on storing vital sign data. The PIMS needs a compatible communication standard to allow storing, and the one most commonly used is the OLE for Process Control (OPC). This paper presents a HL7-OPC Server that permits communication between vital sign monitoring systems with PIMS, thus allowing the storage of long historical series of vital signs. In addition, it carries out a review about local and cloud-based Big Medical Data researches, followed by an analysis of the PIMS in a Health IT Environment. Then it shows the architecture of HL7 and OPC Standards. Finally, it shows the HL7-OPC Server and a sequence of tests that proved its full operation and performance.

In Vitro Antioxidant and Antiproliferative Activities of Novel Orange Peel Extract and It's Fractions on Leukemia HL-60 Cells.

PubMed

Diab, Kawthar A E; Shafik, Reham Ezzat; Yasuda, Shin

2015-01-01

In the present work, novel orange peel was extracted with 100%EtOH (ethanol) and fractionated into four fractions namely F1, F2, F3, F4 which were eluted from paper chromatographs using 100%EtOH, 80%EtOH, 50%EtOH and pure water respectively. The crude extract and its four fractions were evaluated for their total polyphenol content (TPC), total flavonoid content (TFC) and radical scavenging activity using DPPH (1,1-diphenyl-2-picrylhydrazyl) assay. Their cytotoxic activity using WST assay and DNA damage by agarose gel electrophoresis were also evaluated in a human leukemia HL-60 cell line. The findings revealed that F4 had the highest TPC followed by crude extract, F2, F3 and F1. However, the crude extract had the highest TFC followed by F4, F3, F2, and F1. Depending on the values of EC50 and trolox equivalent antioxidant capacity, F4 possessed the strongest antioxidant activity while F1 and F2 displayed weak antioxidant activity. Further, incubation HL-60 cells with extract/fractions for 24h caused an inhibition of cell viability in a concentration- dependent manner. F3 and F4 exhibited a high antiproliferative activity with a narrow range of IC50 values (45.9 - 48.9 μg/ml). Crude extract exhibited the weakest antiproliferative activity with an IC50 value of 314.89 μg/ml. Analysis of DNA fragmentation displayed DNA degradation in the form of a smear-type pattern upon agarose gel after incubation of HL-60 cells with F3 and F4 for 6 h. Overall, F3 and F4 appear to be good sources of phytochemicals with antioxidant and potential anticancer activities.

Polysaccharopeptides derived from Coriolus versicolor potentiate the S-phase specific cytotoxicity of Camptothecin (CPT) on human leukemia HL-60 cells

PubMed Central

2010-01-01

Background Polysaccharopeptide (PSP) from Coriolus versicolor (Yunzhi) is used as a supplementary cancer treatment in Asia. The present study aims to investigate whether PSP pre-treatment can increase the response of the human leukemia HL-60 cells to apoptosis induction by Camptothecin (CPT). Methods We used bivariate bromodeoxyuridine/propidium iodide (BrdUrd/PI) flow cytometry analysis to measure the relative movement (RM) of the BrdUrd positively labeled cells and DNA synthesis time (Ts) on the HL-60 cell line. We used annexin V/PI flow cytometry analysis to quantify the viable, necrotic and apoptotic cells. The expression of cyclin E and cyclin B1 was determined with annexin V/PI flow cytometry and western blotting. Human peripheral blood mononuclear cells were used to test the cytotoxicity of PSP and CPT. Results PSP reduced cellular proliferation; inhibited cells progression through both S and G2 phase, reduced 3H-thymidine uptake and prolonged DNA synthesis time (Ts) in HL-60 cells. PSP-pretreated cells enhanced the cytotoxicity of CPT. The sensitivity of cells to the cytotoxic effects of CPT was seen to be the highest in the S-phase and to a small extent of the G2 phase of the cell cycle. On the other hand, no cell death (measured by annexin V/PI) was evident with the normal human peripheral blood mononuclear cells with treatment of either PSP or CPT. Conclusion The present study shows that PSP increases the sensitization of the HL-60 cells to undergo effective apoptotic cell death induced by CPT. The pattern of sensitivity of cancer cells is similar to that of HL-60 cells. PSP rapidly arrests and/or kills cells in S-phase and did not interfere with the anticancer action of CPT. PSP is a potential adjuvant to treat human leukemia as rapidly proliferating tumors is characterized by a high proportion of S-phase cells. PMID:20423495

Effect of lift-to-drag ratio in pilot rating of the HL-20 landing task

NASA Technical Reports Server (NTRS)

Jackson, E. B.; Rivers, Robert A.; Bailey, Melvin L.

1993-01-01

A man-in-the-loop simulation study of the handling qualities of the HL-20 lifting-body vehicle was made in a fixed-base simulation cockpit at NASA Langley Research Center. The purpose of the study was to identify and substantiate opportunities for improving the original design of the vehicle from a handling qualities and landing performance perspective. Using preliminary wind-tunnel data, a subsonic aerodynamic model of the HL-20 was developed. This model was adequate to simulate the last 75-90 s of the approach and landing. A simple flight-control system was designed and implemented. Using this aerodynamic model as a baseline, visual approaches and landings were made at several vehicle lift-to-drag ratios. Pilots rated the handling characteristics of each configuration using a conventional numerical pilot-rating scale. Results from the study showed a high degree of correlation between the lift-to-drag ratio and pilot rating. Level 1 pilot ratings were obtained when the L/D ratio was approximately 3.8 or higher.

Effect of lift-to-drag ratio in pilot rating of the HL-20 landing task

NASA Astrophysics Data System (ADS)

Jackson, E. B.; Rivers, Robert A.; Bailey, Melvin L.

1993-10-01

A man-in-the-loop simulation study of the handling qualities of the HL-20 lifting-body vehicle was made in a fixed-base simulation cockpit at NASA Langley Research Center. The purpose of the study was to identify and substantiate opportunities for improving the original design of the vehicle from a handling qualities and landing performance perspective. Using preliminary wind-tunnel data, a subsonic aerodynamic model of the HL-20 was developed. This model was adequate to simulate the last 75-90 s of the approach and landing. A simple flight-control system was designed and implemented. Using this aerodynamic model as a baseline, visual approaches and landings were made at several vehicle lift-to-drag ratios. Pilots rated the handling characteristics of each configuration using a conventional numerical pilot-rating scale. Results from the study showed a high degree of correlation between the lift-to-drag ratio and pilot rating. Level 1 pilot ratings were obtained when the L/D ratio was approximately 3.8 or higher.

Optimal Control Techniques for ResistiveWall Modes in Tokamaks

NASA Astrophysics Data System (ADS)

Clement, Mitchell Dobbs Pearson

Tokamaks can excite kink modes that can lock or nearly lock to the vacuum vessel wall, and whose rotation frequencies and growth rates vary in time but are generally inversely proportional to the magnetic flux diffusion time of the vacuum vessel wall. This magnetohydrodynamic (MHD) instability is pressure limiting in tokamaks and is called the Resistive Wall Mode (RWM). Future tokamaks that are expected to operate as fusion reactors will be required to maximize plasma pressure in order to maximize fusion performance. The DIII-D tokamak is equipped with electromagnetic control coils, both inside and outside of its vacuum vessel, which create magnetic fields that are small by comparison to the machine's equilibrium field but are able to dynamically counteract the RWM. Presently for RWM feedback, DIII-D uses its interior control coils using a classical proportional gain only controller to achieve high plasma pressure. Future advanced tokamak designs will not likely have the luxury of interior control coils and a proportional gain algorithm is not expected to be effective with external control coils. The computer code VALEN was designed to calculate the performance of an MHD feedback control system in an arbitrary geometry. VALEN models the perturbed magnetic field from a single MHD instability and its interaction with surrounding conducting structures using a finite element approach. A linear quadratic gaussian (LQG) control, or H 2 optimal control, algorithm based on the VALEN model for RWM feedback was developed for use with DIII-D's external control coil set. The algorithm is implemented on a platform that combines a graphics processing unit (GPU) for real-time control computation with low latency digital input/output control hardware and operates in parallel with the DIII-D Plasma Control System (PCS). Simulations and experiments showed that modern control techniques performed better, using 77% less current, than classical techniques when using coils external to

A Selenium-Modified Ginseng Polysaccharide Promotes the Apoptosis in Human Promyelocytic Leukemia (HL-60) Cells via a Mitochondrial-Mediated Pathway.

PubMed

Liao, Kainan; Bian, Zedong; Xie, Dongke; Peng, Qiang

2017-05-01

A ginseng polysaccharide was extracted, purified, and modified by nitric acid-selenious acid (HNO 3 -H 2 SeO 3 ) method to yield one selenylation-modified polysaccharide (sGP). We reported for the first time the anticancer potential of sGP on the human promyelocytic leukemia HL-60 cell line and evaluated its relevant underlying mechanism. Our results showed that sGP markedly inhibited the growth of HL-60 cells via induction of apoptosis. The event of apoptosis was accompanied by the formation of apoptotic bodies; the release of cytochrome c; loss of mitochondrial membrane potential; and activation of caspase-9, caspase-3, and cleavage of poly ADP ribose polymerase (PARP) in HL-60 cells. In addition, western blot analysis showed that sGP inhibited antiapoptotic Bcl-2 protein expression and increased proapoptotic Bax protein expression in cells under identical conditions. Together, our study suggests that sGP induces apoptosis of HL-60 cells through the mitochondrial-dependent pathway.

Leptin regulates MMP-2, TIMP-1 and collagen synthesis via p38 MAPK in HL-1 murine cardiomyocytes.

PubMed

Schram, Kristin; De Girolamo, Sabrina; Madani, Siham; Munoz, Diana; Thong, Farah; Sweeney, Gary

2010-12-01

A clear association between obesity and heart failure exists and a significant role for leptin, the product of the obese gene, has been suggested. One aspect of myocardial remodeling which characterizes heart failure is a disruption in the balance of extracellular matrix synthesis and degradation. Here we investigated the effects of leptin on matrix metalloproteinase (MMP) activity, tissue inhibitor of metalloproteinase (TIMP) expression, as well as collagen synthesis in HL-1 cardiac muscle cells. Gelatin zymographic analysis of MMP activity in conditioned media showed that leptin enhanced MMP-2 activity in a dose- and time-dependent manner. Leptin is known to stimulate phosphorylation of p38 MAPK in cardiac cells and utilization of the p38 MAPK inhibitor, SB203580, demonstrated that this kinase also plays a role in regulating several extracellular matrix components, such that inhibition of p38 MAPK signaling prevented the leptin-induced increase in MMP-2 activation. We also observed that leptin enhanced collagen synthesis determined by both proline incorporation and picrosirius red staining of conditioned media. Pro-collagen type-I and pro-collagen type-III expression, measured by real-time PCR and Western blotting were also increased by leptin, effects which were again attenuated by SB203580. In summary, these results demonstrate the potential for leptin to play a role in mediating myocardial ECM remodeling and that the p38 MAPK pathway plays an important role in mediating these effects.

Aero Spacelines B377SG Super Guppy on Ramp Loading the X-24B and HL-10 Lifting Bodies.

NASA Technical Reports Server (NTRS)

1976-01-01

flight on March 19, 1970. The X-24A was flown 28 times in the program that, like the HL-10, validated the concept that a Space Shuttle vehicle could be landed unpowered. The fastest speed achieved by the X-24A was 1,036 miles per hour (mph--Mach 1.6). Its maximum altitude was 71,400 feet. It was powered by an XLR-11 rocket engine with a maximum theoretical vacuum thrust of 8,480 pounds. The X-24A was later modified into the X-24B. The bulbous shape of the X-24A was converted into a 'flying flatiron' shape with a rounded top, flat bottom, and double delta platform that ended in a pointed nose. The X-24B demonstrated that accurate unpowered reentry vehicle landings were operationally feasible. Top speed achieved by the X-24B was 1,164 mph and the highest altitude it reached was 74,130 feet. The vehicle is on display at the Air Force Museum, Wright-Patterson Air Force Base, Ohio. The pilot on the last powered flight of the X-24B was Bill Dana, who also flew the last X-15 flight about seven years earlier. The X-24A shape was later borrowed for the X-38 Crew Return Vehicle (CRV) technology demonstrator for the International Space Station. The X-24B is on public display at the Air Force Museum, Wright-Patterson AFB, Ohio. The HL-10 was delivered to the FRC by Northrop in January 1966. Its first flight was on Dec. 22 of the same year. The pilot was Bruce Peterson. The HL-10 was flown 37 times and it set several program records. On Feb. 18, 1970, Air Force test pilot Maj. Peter Hoag flew it to 1,228 mph (Mach 1.86), fastest speed of any of the lifting bodies. Nine days later, NASA's Bill Dana flew the HL-10 to 90,303 feet, the highest altitude reached by any of the lifting body vehicles. The HL-10 was also the first lifting body to fly supersonically--on May 9, 1969, with Manke at the controls. The HL-10 featured a flat bottom and rounded top -- much like an airfoil -- and it had a delta planform. In its final configuration, three vertical fins, two of them canted outwards from

Interoperability of medical device information and the clinical applications: an HL7 RMIM based on the ISO/IEEE 11073 DIM.

PubMed

Yuksel, Mustafa; Dogac, Asuman

2011-07-01

Medical devices are essential to the practice of modern healthcare services. Their benefits will increase if clinical software applications can seamlessly acquire the medical device data. The need to represent medical device observations in a format that can be consumable by clinical applications has already been recognized by the industry. Yet, the solutions proposed involve bilateral mappings from the ISO/IEEE 11073 Domain Information Model (DIM) to specific message or document standards. Considering that there are many different types of clinical applications such as the electronic health record and the personal health record systems, the clinical workflows, and the clinical decision support systems each conforming to different standard interfaces, detailing a mapping mechanism for every one of them introduces significant work and, thus, limits the potential health benefits of medical devices. In this paper, to facilitate the interoperability of clinical applications and the medical device data, we use the ISO/IEEE 11073 DIM to derive an HL7 v3 Refined Message Information Model (RMIM) of the medical device domain from the HL7 v3 Reference Information Mode (RIM). This makes it possible to trace the medical device data back to a standard common denominator, that is, HL7 v3 RIM from which all the other medical domains under HL7 v3 are derived. Hence, once the medical device data are obtained in the RMIM format, it can easily be transformed into HL7-based standard interfaces through XML transformations because these interfaces all have their building blocks from the same RIM. To demonstrate this, we provide the mappings from the developed RMIM to some of the widely used HL7 v3-based standard interfaces.

Multi-frequency ICRF diagnostic of Tokamak plasmas

NASA Astrophysics Data System (ADS)

Lafonteese, David James

This thesis explores the diagnostic possibilities of a fast wave-based method for measuring the ion density and temperature profiles of tokamak plasmas. In these studies fast waves are coupled to the plasma at frequencies at the second harmonic of the ion gyrofrequency, at which wave energy is absorbed by the finite-temperature ions. As the ion gyrofrequency is dependent upon the local magnetic field, which varies as l/R in a tokamak, this power absorption is radially localized. The simultaneous launching of multiple frequencies, all resonating at different plasma positions, allows local measurements of the ion density and temperature. To investigate the profile applications of wave damping measurements in a simulated tokamak, an inhouse slab-model ICRF code is developed. A variety of analysis methods are presented, and ion density and temperature profiles are reconstructed for hydrogen plasmas for the Electric Tokamak (ET) and ITER parameter spaces. These methods achieve promising results in simulated plasmas featuring bulk ion heating, off-axis RF heating, and density ramps. The experimental results of similar studies on the Electric Tokamak, a high aspect ratio (R/a = 5), low toroidal field (2.2 kG) device are then presented. In these studies, six fast wave frequencies were coupled using a single-strap, low-field-side antenna to ET plasmas. The frequencies were variable, and could be tuned to resonate at different radii for different experiments. Four magnetic pickup loops were used to measure of the toroidal component of the wave magnetic field. The expected greater eigenmode damping of center-resonant frequencies versus edge-resonant frequencies is consistently observed. Comparison of measured aspects of fast wave behavior in ET is made with the slab code predictions, which validate the code simulations under weakly-damped conditions. A density profile is measured for an ET discharge through analysis of the fast wave measurements, and is compared to an

Regulated expression of the MRP8 and MRP14 genes in human promyelocytic leukemic HL-60 cell treated with the differentiation-inducing agents mycophenolic acid and 1{alpha},25-Dihydroxyvitamin D{sub 3}

DOE Office of Scientific and Technical Information (OSTI.GOV)

Warner-Bartnicki, A.L.; Murao, S.; Collart, F.R.

1992-12-31

The calcium-binding proteins MRP8 and MEP14 are present in mature monomyelocytic cells and are induced during differentiation. Previous studies have demonstrated that the proteins may mediate the growth arrest in differentiating HL-60 cells. We determined the levels of a protein complex (PC) containing MRP8 and MRP14 and investigated the mechanism by which the genes encoding these proteins are regulated in HL-60 cells treated with the differentiation-inducing agent mycophenorc acid (MPA)While the PC was barely detectable in untreated cells, MPA treatment resulted in elevated levels of the PC which were maximal at 3-4 d, and were found to directly parallel gainsmore » in the steady-state levels of MRP8 and MRP14 MRNA. Transcription studies with the use of nuclear run-on experiments revealed increased transcription initiation at the MRP8 and MRP14 promoters after MPA treatment. 1{alpha},25-Dihydroxyvitamin D{sub 3}, which induces HL-60 cell differentiation by another mechanism, was also found to increase transcription initiation at the MRP8 and MRP14 promoters. Our results suggest that this initiation is the major control of maturation agent-mediated increases in MRP8 and MRPl4 gene expression, and support a role for the PC in terminal differentiation of human monomyelocytic cells.« less

Protection of tokamak plasma facing components by a capillary porous system with lithium

NASA Astrophysics Data System (ADS)

Lyublinski, I.; Vertkov, A.; Mirnov, S.; Lazarev, V.

2015-08-01

Development of plasma facing material (PFM) based on the Capillary-Porous System (CPS) with lithium and activity on realization of lithium application strategy are addressed to meet the challenges under the creation of steady-state tokamak fusion reactor and fusion neutron source. Presented overview of experimental study of lithium CPS in plasma devices demonstrates the progress in protection of tokamak plasma facing components (PFC) from damage, stabilization and self-renewal of liquid lithium surface, elimination of plasma pollution and lithium accumulation in tokamak chamber. The possibility of PFC protection from the high power load related to cooling of the tokamak boundary plasma by radiation of non-fully stripped lithium ions supported by experimental results. This approach demonstrated in scheme of closed loops of Li circulation in the tokamak vacuum chamber and realized in a series of design of tokamak in-vessel elements.

EVIDENCE OF FAST PEBBLE GROWTH NEAR CONDENSATION FRONTS IN THE HL TAU PROTOPLANETARY DISK

DOE Office of Scientific and Technical Information (OSTI.GOV)

Zhang, Ke; Blake, Geoffrey A.; Bergin, Edwin A., E-mail: kzhang@astro.caltech.edu

2015-06-10

Water and simple organic molecular ices dominate the mass of solid materials available for planetesimal and planet formation beyond the water snow line. Here we analyze ALMA long baseline 2.9, 1.3 and 0.87 mm continuum images of the young star HL Tau, and suggest that the emission dips observed are due to rapid pebble growth around the condensation fronts of abundant volatile species. Specifically, we show that the prominent innermost dip at 13 AU is spatially resolved in the 0.87 mm image, and its center radius is coincident with the expected mid-plane condensation front of water ice. In addition, twomore » other prominent dips, at distances of 32 and 63 AU, cover the mid-plane condensation fronts of pure ammonia or ammonia hydrates and clathrate hydrates (especially with CO and N{sub 2}) formed from amorphous water ice. The spectral index map of HL Tau between 1.3 and 0.87 mm shows that the flux ratios inside the dips are statistically larger than those of nearby regions in the disk. This variation can be explained by a model with two dust populations, where most of the solid mass resides in a component that has grown to decimeter size scales inside the dips. Such growth is in accord with recent numerical simulations of volatile condensation, dust coagulation, and settling.« less

Dust-Particle Transport in Tokamak Edge Plasmas

DOE Office of Scientific and Technical Information (OSTI.GOV)

Pigarov, A Y; Krasheninnikov, S I; Soboleva, T K

2005-09-12

Dust particulates in the size range of 10nm-100{micro}m are found in all fusion devices. Such dust can be generated during tokamak operation due to strong plasma/material-surface interactions. Some recent experiments and theoretical estimates indicate that dust particles can provide an important source of impurities in the tokamak plasma. Moreover, dust can be a serious threat to the safety of next-step fusion devices. In this paper, recent experimental observations on dust in fusion devices are reviewed. A physical model for dust transport simulation, and a newly developed code DUSTT, are discussed. The DUSTT code incorporates both dust dynamics due to comprehensivemore » dust-plasma interactions as well as the effects of dust heating, charging, and evaporation. The code tracks test dust particles in realistic plasma backgrounds as provided by edge-plasma transport codes. Results are presented for dust transport in current and next-step tokamaks. The effect of dust on divertor plasma profiles and core plasma contamination is examined.« less

Tokamak startup using point-source dc helicity injection.

PubMed

Battaglia, D J; Bongard, M W; Fonck, R J; Redd, A J; Sontag, A C

2009-06-05

Startup of a 0.1 MA tokamak plasma is demonstrated on the ultralow aspect ratio Pegasus Toroidal Experiment using three localized, high-current density sources mounted near the outboard midplane. The injected open field current relaxes via helicity-conserving magnetic turbulence into a tokamaklike magnetic topology where the maximum sustained plasma current is determined by helicity balance and the requirements for magnetic relaxation.

Sensitivity of magnetic field-line pitch angle measurements to sawtooth events in tokamaks

DOE Office of Scientific and Technical Information (OSTI.GOV)

Ko, J., E-mail: jinseok@nfri.re.kr

2016-11-15

The sensitivity of the pitch angle profiles measured by the motional Stark effect (MSE) diagnostic to the evolution of the safety factor, q, profiles during the tokamak sawtooth events has been investigated for Korea Superconducting Tokamak Advanced Research (KSTAR). An analytic relation between the tokamak pitch angle, γ, and q estimates that Δγ ∼ 0.1° is required for detecting Δq ∼ 0.05 near the magnetic axis (not at the magnetic axis, though). The pitch angle becomes less sensitive to the same Δq for the middle and outer regions of the plasma (Δγ ∼ 0.5°). At the magnetic axis, it ismore » not straightforward to directly relate the γ sensitivity to Δq since the gradient of γ(R), where R is the major radius of the tokamak, is involved. Many of the MSE data obtained from the 2015 KSTAR campaign, when calibrated carefully, can meet these requirements with the time integration down to 10 ms. The analysis with the measured data shows that the pitch angle profiles and their gradients near the magnetic axis can resolve the change of the q profiles including the central safety factor, q{sub 0}, during the sawtooth events.« less

Probing spherical tokamak plasmas using charged fusion products

NASA Astrophysics Data System (ADS)

Boeglin, Werner U.; Perez, Ramona V.; Darrow, Douglass S.; Cecconello, Marco; Klimek, Iwona; Allan, Scott Y.; Akers, Rob J.; Jones, Owen M.; Keeling, David L.; McClements, Ken G.; Scannell, Rory

2015-11-01

The detection of charged fusion products, such as protons and tritons resulting from D(d,p)t reactions, can be used to determine the fusion reaction rate profile in large spherical tokamak plasmas with neutral beam heating. The time resolution of a diagnostic of this type makes it possible to study the slowly-varying beam density profile, as well as rapid changes resulting from MHD instabilities. A 4-channel prototype proton detector (PD) was installed and operated on the MAST spherical tokamak in August/September 2013, and a new 6-channel system for the NSTX-U spherical tokamak is under construction. PD and neutron camera measurements obtained on MAST will be compared with TRANSP calculations, and the design of the new NSTX-U system will be presented, together with the first results from this diagnostic, if available. Supported in part by DOE DE-SC0001157.

[Down-regulatory effect of Nucleostemin expression on signal molecule of PI3K/AKT/mTOR pathway in HL-60 cells].

PubMed

Jia, Yu; Wei, Yuan-Yu; Zhang, Fan; Li, Zhao-Bo; Liu, Shuai; Yue, Bao-Hong

2014-02-01

This study was purpose to explore the down-regulatory effect of nucleostemin (NS) expression on signal molecules of PI3K/AKT/mTOR pathway belonged to candidate ways of p53-independent signal pathway in the leukemia cells. The expression of NS was interfered by using recombinant lentivirus expression vector NS-RNAi-GV248 to transfect HL-60 cells of p53 deficiency. The expression of NS and signal molecules of PI3K/AKT/mTOR pathway were detected by using Real-time PCR. The results of showed that the HL-60 cells were transfected by recombinant lentivirus vector NS-RNAi-GV248 successfully and with transfection rate up to 80%. According to results of Real-time PCR detection, the inhibition rate of NS gene was 56.5% in HL-60 cells. And the expression levels of PI3K,AKT and GβL mRNA (0.491 ± 0.084,0.398 ± 0.164, 0.472 ± 0.097 respectively) were obviously down-regulated by silencing NS, and showed statistical difference (P < 0.05) in comparison with control (1.002 ± 0.171, 1.000 ± 0.411, 1.001 ± 0.206 respectively) . It is concluded that the changes of signal molecules of PI3K/AKT/mTOR pathway positively correlate with NS down-regulation, which provides evidence for confirming PI3K/AKT/mTOR signal pathway possible as a type of NS p53-independent pathway.

Sub-Alfvénic reduced magnetohydrodynamic equations for tokamaks

NASA Astrophysics Data System (ADS)

Sengupta, W.; Hassam, A. B.; Antonsen, T. M.

2017-06-01

A reduced set of magnetohydrodynamic (MHD) equations is derived, applicable to large aspect ratio tokamaks and relevant for dynamics that is sub-Alfvénic with respect to ideal ballooning modes. This ordering optimally allows sound waves, Mercier modes, drift modes, geodesic-acoustic modes (GAM), zonal flows and shear Alfvén waves. Wavelengths long compared to the gyroradius but comparable to the minor radius of a typical tokamak are considered. With the inclusion of resistivity, tearing modes, resistive ballooning modes, Pfirsch-Schluter cells and the Stringer spin-up are also included. A major advantage is that the resulting system is two-dimensional in space, and the system incorporates self-consistent and dynamic Shafranov shifts. A limitation is that the system is valid only in radial domains where the tokamak safety factor, , is close to rational. In the tokamak core, the system is well suited to study the sawtooth discharge in the presence of Mercier modes. The systematic ordering scheme and methodology developed are versatile enough to reduce the more general collisional two-fluid equations or possibly the Vlasov-Maxwell system in the MHD ordering.

Effects of Hot Limiter Biasing on Tokamak Runaway Discharges

NASA Astrophysics Data System (ADS)

Salar Elahi, A.; Ghoranneviss, M.; Ghanbari, M. R.

2013-10-01

In this research hot limiter biasing effects on the Runaway discharges were investigated. First wall of the tokamak reactors can affects serious damage due to the high energy runaway electrons during a major disruption and therefore its life time can be reduced. Therefore, it is important to find methods to decrease runaway electron generation and their energy. Tokamak limiter biasing is one of the methods for controlling the radial electric field and can induce a transition to an improved confinement state. In this article generation of runaway electrons and the energy they can obtain will be investigated theoretically. Moreover, in order to apply radial biasing an emissive limiter biasing is utilized. The biased limiter can apply +380 V in the status of cold and hot to the plasma and result in the increase of negative bias current in hot status. In fact, in this experiment we try to decrease the generation of runaway electrons and their energy by using emissive limiter biasing inserted on the IR-T1 tokamak. The mean energy of these electrons was obtained by spectroscopy of hard X-ray. Also, the plasma current center shift was measured from the vertical field coil characteristics in presence of limiter biasing. The calculation is made focusing on the vertical field coil current and voltage changes due to a horizontal displacement of plasma column.

Developing and flight testing the HL-10 lifting body: A precursor to the Space Shuttle

NASA Technical Reports Server (NTRS)

Kempel, Robert W.; Painter, Weneth D.; Thompson, Milton O.

1994-01-01

The origins of the lifting-body idea are traced back to the mid-1950's, when the concept of a manned satellite reentering the Earth's atmosphere in the form of a wingless lifting body was first proposed. The advantages of low reentry deceleration loads, range capability, and horizontal landing of a lifting reentry vehicle (as compared with the high deceleration loads and parachute landing of a capsule) are presented. The evolution of the hypersonic HL-10 lifting body is reviewed from the theoretical design and development process to its selection as one of two low-speed flight vehicles for fabrication and piloted flight testing. The design, development, and flight testing of the low-speed, air-launched, rocket-powered HL-10 was part of an unprecedented NASA and contractor effort. NASA Langley Research Center conceived and developed the vehicle shape and conducted numerous theoretical, experimental, and wind-tunnel studies. NASA Flight Research Center (now NASA Dryden Flight Research Center) was responsible for final low-speed (Mach numbers less than 2.0) aerodynamic analysis, piloted simulation, control law development, and flight tests. The prime contractor, Northrop Corp., was responsible for hardware design, fabrication, and integration. Interesting and unusual events in the flight testing are presented with a review of significant problems encountered in the first flight and how they were solved. Impressions by the pilots who flew the HL-10 are included. The HL-10 completed a successful 37-flight program, achieved the highest Mach number and altitude of this class vehicle, and contributed to the technology base used to develop the space shuttle and future generations of lifting bodies.

Destruction of tungsten limiters in the T-10 Tokamak under high plasma heat loads

NASA Astrophysics Data System (ADS)

Grashin, S. A.; Arkhipov, I. I.; Budaev, V. P.; Giniyatulin, R. N.; Karpov, A. V.; Klyuchnikov, L. A.; Krupin, V. A.; Litunovskiy, N. V.; Masul, I. V.; Makhankov, F. N.; Martynenko, Yu V.; Sarytchev, D. V.; Solomatin, R. Yu; Khimchenko, L. N.

2017-10-01

Tungsten limiters were tested in the T-10 tokamak. The limiters were made from the ITER-grade WMP “POLEMA” tungsten. The influence of the edge tokamak plasma on tungsten limiters leads to significant cracking of tungsten. The heat load of up to 2 MW · m-2 leads to the micro-crack development at the grain boundaries accompanied by the loss of grains. The heat loads that exceed 5 MW · m-2 lead to the macro crack development. Under the present T-10 tokamak conditions, the heat and particle fluxes in the edge plasma lead to the significant destruction of tungsten limiters during the experimental campaign. During the disruption and runaway electron formation, extreme heat loads of more than 1 GW/m2 cause strong melting of tungsten on the inner and outer part of the ring limiter.

Dose- and Time-Dependent Response of Human Leukemia (HL-60) Cells to Arsenic Trioxide Treatment

PubMed Central

Yedjou, Clement G.; Moore, Pamela; Tchounwou, Paul B.

2006-01-01

The treatment of acute promyelocytic leukemia (APL) has been based on the administration of all-trans retinoic acid plus anthracycline chemotherapy, which is very effective as first line therapy; however 25 to 30% of patients will relapse with their disease becoming refractory to conventional therapy. Recently, studies have shown arsenic trioxide to be effective in the treatment of acute promyelocytic leukemia. In this study, we used the human leukemia (HL-60) cell line as a model to evaluate the cytoxicity of arsenic trioxide based on the MTT assay. Data obtained from this assay indicated that arsenic trioxide significantly reduced the viability of HL-60 cells, showing LD50 values of 14.26 ± 0.5μg/mL, 12.54 ± 0.3μg/mL, and 6.4 ± 0.6μg/mL upon 6, 12, and 24 hours of exposure, respectively; indicating a dose- and time-dependent response relationship. Findings from the present study indicate that arsenic trioxide is highly cytotoxic to human leukemia (HL-60) cells, supporting its use as an effective therapeutic agent in the management of acute promyelocytic leukemia. PMID:16823087

Dynamic diagnostics of the error fields in tokamaks

NASA Astrophysics Data System (ADS)

Pustovitov, V. D.

2007-07-01

The error field diagnostics based on magnetic measurements outside the plasma is discussed. The analysed methods rely on measuring the plasma dynamic response to the finite-amplitude external magnetic perturbations, which are the error fields and the pre-programmed probing pulses. Such pulses can be created by the coils designed for static error field correction and for stabilization of the resistive wall modes, the technique developed and applied in several tokamaks, including DIII-D and JET. Here analysis is based on the theory predictions for the resonant field amplification (RFA). To achieve the desired level of the error field correction in tokamaks, the diagnostics must be sensitive to signals of several Gauss. Therefore, part of the measurements should be performed near the plasma stability boundary, where the RFA effect is stronger. While the proximity to the marginal stability is important, the absolute values of plasma parameters are not. This means that the necessary measurements can be done in the diagnostic discharges with parameters below the nominal operating regimes, with the stability boundary intentionally lowered. The estimates for ITER are presented. The discussed diagnostics can be tested in dedicated experiments in existing tokamaks. The diagnostics can be considered as an extension of the 'active MHD spectroscopy' used recently in the DIII-D tokamak and the EXTRAP T2R reversed field pinch.

Development of High vacuum facility for baking and cool down experiments for SST-1 Tokamak components

NASA Astrophysics Data System (ADS)

Khan, Ziauddin; Pathan, Firozkhan S.; Yuvakiran, Paravastu; George, Siju; Manthena, Himabindu; Raval, Dilip C.; Thankey, Prashant L.; Dhanani, Kalpesh R.; Gupta, Manoj Kumar; Pradhan, Subrata

2012-11-01

SST-1 Tokamak, a steady state super-conducting device, is under refurbishment to demonstrate the plasma discharge for the duration of 1000 second. The major fabricated components of SST-1 like vacuum vessel, thermal shields, superconducting magnets etc have to be tested for their functional parameters. During machine operation, vacuum vessel will be baked at 150 °C, thermal shields will be operated at 85 K and magnet system will be operated at 4.5 K. All these components must have helium leak tightness under these conditions so far as the machine operation is concerned. In order to validate the helium leak tightness of these components, in-house high vacuum chamber is fabricated. This paper describes the analysis, design and fabrication of high vacuum chamber to demonstrate these functionalities. Also some results will be presented.

Source-to-incident-flux relation in a Tokamak blanket module

NASA Astrophysics Data System (ADS)

Imel, G. R.

The next-generation Tokamak experiments, including the Tokamak fusion test reactor (TFTR), will utilize small blanket modules to measure performance parameters such as tritium breeding profiles, power deposition profiles, and neutron flux profiles. Specifically, a neutron calorimeter (simply a neutron moderating blanket module) which permits inferring the incident 14 MeV flux based on measured temperature profiles was proposed for TFTR. The problem of how to relate this total scalar flux to the fusion neutron source is addressed. This relation is necessary since the calorimeter is proposed as a total fusion energy monitor. The methods and assumptions presented was valid for the TFTR Lithium Breeding Module (LBM), as well as other modules on larger Tokamak reactors.

Plasma production and preliminary results from the ADITYA Upgrade tokamak

NASA Astrophysics Data System (ADS)

R, L. TANNA; J, GHOSH; Harshita, RAJ; Rohit, KUMAR; Suman, AICH; Vaibhav, RANJAN; K, A. JADEJA; K, M. PATEL; S, B. BHATT; K, SATHYANARAYANA; P, K. CHATTOPADHYAY; M, N. MAKWANA; K, S. SHAH; C, N. GUPTA; V, K. PANCHAL; Praveenlal, EDAPPALA; Bharat, ARAMBHADIYA; Minsha, SHAH; Vismay, RAULJI; M, B. CHOWDHURI; S, BANERJEE; R, MANCHANDA; D, RAJU; P, K. ATREY; Umesh, NAGORA; J, RAVAL; Y, S. JOISA; K, TAHILIANI; S, K. JHA; M, V. GOPALKRISHANA

2018-07-01

The Ohmically heated circular limiter tokamak ADITYA (R 0 = 75 cm, a = 25 cm) has been upgraded to a tokamak named the ADITYA Upgrade (ADITYA-U) with an open divertor configuration with divertor plates. The main goal of ADITYA-U is to carry out dedicated experiments relevant for bigger fusion machines including ITER, such as the generation and control of runaway electrons, disruption prediction, and mitigation studies, along with an improvement in confinement with shaped plasma. The ADITYA tokamak was dismantled and the assembly of ADITYA-U was completed in March 2016. Integration of subsystems like data acquisition and remote operation along with plasma production and preliminary plasma characterization of ADITYA-U plasmas are presented in this paper.

Effects of extracts of Coriolus versicolor (I'm-Yunity) on cell-cycle progression and expression of interleukins-1 beta,-6, and -8 in promyelocytic HL-60 leukemic cells and mitogenically stimulated and nonstimulated human lymphocytes.

PubMed

Hsieh, Tze-Chen; Kunicki, Jan; Darzynkiewicz, Zbigniew; Wu, Joseph M

2002-10-01

The goal of this in vitro study was to test the cytostatic and cytotoxic activities of extracts derived from the polysaccharopeptide (PSP), I'm-Yunity (Integrated Chinese Medicine Holdings Ltd., Kowloon, Hong Kong) prepared from strain Cov-1 of the mushroom Coriolus versicolor. Different volumes of 70% ethanol and water extracts of I'm-Yunity were incubated with cultures of human promyelocytic leukemic HL-60 cells, and compared to nontreated control cells. At various times after treatment, cells were harvested and analyzed with respect to: (1). proliferation and cell cycle phase distribution, (2). induction of apoptosis, and (3). changes in expression of the immunomodulating cytokines interleukin (IL)-1 beta, IL-6, and IL-8. To test whether extracts also affected normal cells, similar experiments were also performed using isolated peripheral blood lymphocytes from healthy volunteers, with and without stimulation by the mitogen phytohemagglutinin (PHA). The ability of extracts to affect the secretion of IL-1 beta, IL-6, and IL-8 were assessed by enzyme-linked immunosorbent assay. HL-60 cells incubated with various amounts (1, 3, 5, 7.5, and 10 micro l/mL) of the extracts for 1-3 days showed dose-dependent, time-dependent growth suppression and decrease in cell viability. Flow cytometric analysis revealed partial cell arrest in the G(1) phase at less than 5 micro L/mL and induction of apoptosis at 10 micro L/mL or more of ethanol and water extracts, with the latter exhibiting more pronounced inhibition than the former. Experiments performed with lymphocytes demonstrated that extracts of I'm-Yunity alone were without effect; moreover, they also did not affect the lymphocyte response to PHA. Water extract of I'm-Yunity also significantly increased IL-1 beta and IL-6 while substantially lowering IL-8. I'm-Yunity acts selectively in HL-60 leukemic cells, resulting in cell cycle restriction through the G(1)/S checkpoint and the induction of apoptosis.

Data Warehouse Design from HL7 Clinical Document Architecture Schema.

PubMed

Pecoraro, Fabrizio; Luzi, Daniela; Ricci, Fabrizio L

2015-01-01

This paper proposes a semi-automatic approach to extract clinical information structured in a HL7 Clinical Document Architecture (CDA) and transform it in a data warehouse dimensional model schema. It is based on a conceptual framework published in a previous work that maps the dimensional model primitives with CDA elements. Its feasibility is demonstrated providing a case study based on the analysis of vital signs gathered during laboratory tests.

Design and fabrication of the NASA HL-20 full scale research model

NASA Technical Reports Server (NTRS)

Driver, K. Dean; Vess, Robert J.

1991-01-01

A full-scale engineering model of the HL-20 Personnel Launch System (PLS) was constructed for systems and human factors evaluation. Construction techniques were developed to enable the vehicle to be constructed with a minimum of time and cost. The design and construction of the vehicle are described.

Interaction of external n = 1 magnetic fields with the sawtooth instability in low- q RFX-mod and DIII-D tokamaks

DOE PAGES

Piron, C.; Martin, P.; Bonfiglio, D.; ...

2016-08-11

External n = 1 magnetic fields are applied in RFX-mod and DIII-D low safety factor Tokamak plasmas to investigate their interaction with the internal MHD dynamics and in particular with the sawtooth instability. In these experiments the applied magnetic fields cause a reduction of both the sawtooth amplitude and period, leading to an overall stabilizing effect on the oscillations. In RFX-mod sawteeth eventually disappear and are replaced by a stationary m = 1, n = 1 helical equilibrium without an increase in disruptivity. However toroidal rotation is significantly reduced in these plasmas, thus it is likely that the sawtooth mitigationmore » in these experiments is due to the combination of the helically deformed core and the reduced rotation. The former effect is qualitatively well reproduced by nonlinear MHD simulations performed with the PIXIE3D code. The results obtained in these RFX-mod experiments motivated similar ones in DIII-D L-mode diverted Tokamak plasmas at low q 95. These experiments succeeded in reproducing the sawtooth mitigation with the approach developed in RFX-mod. In DIII-D this effect is correlated with a clear increase of the n = 1 plasma response, that indicates an enhancement of the coupling to the marginally stable n = 1 external kink, as simulations with the linear MHD code IPEC suggest. A significant rotation braking in the plasma core is also observed in DIII-D. Finally, numerical calculations of the neoclassical toroidal viscosity (NTV) carried out with PENT identify this torque as a possible contributor for this effect.« less

ADX - Advanced Divertor and RF Tokamak Experiment

NASA Astrophysics Data System (ADS)

Greenwald, Martin; Labombard, Brian; Bonoli, Paul; Irby, Jim; Terry, Jim; Wallace, Greg; Vieira, Rui; Whyte, Dennis; Wolfe, Steve; Wukitch, Steve; Marmar, Earl

2015-11-01

The Advanced Divertor and RF Tokamak Experiment (ADX) is a design concept for a compact high-field tokamak that would address boundary plasma and plasma-material interaction physics challenges whose solution is critical for the viability of magnetic fusion energy. This device would have two crucial missions. First, it would serve as a Divertor Test Tokamak, developing divertor geometries, materials and operational scenarios that could meet the stringent requirements imposed in a fusion power plant. By operating at high field, ADX would address this problem at a level of power loading and other plasma conditions that are essentially identical to those expected in a future reactor. Secondly, ADX would investigate the physics and engineering of high-field-side launch of RF waves for current drive and heating. Efficient current drive is an essential element for achieving steady-state in a practical, power producing fusion device and high-field launch offers the prospect of higher efficiency, better control of the current profile and survivability of the launching structures. ADX would carry out this research in integrated scenarios that simultaneously demonstrate the required boundary regimes consistent with efficient current drive and core performance.

The Dynamic Mutation Characteristics of Thermonuclear Reaction in Tokamak

PubMed Central

Li, Jing; Quan, Tingting; Zhang, Wei; Deng, Wei

2014-01-01

The stability and bifurcations of multiple limit cycles for the physical model of thermonuclear reaction in Tokamak are investigated in this paper. The one-dimensional Ginzburg-Landau type perturbed diffusion equations for the density of the plasma and the radial electric field near the plasma edge in Tokamak are established. First, the equations are transformed to the average equations with the method of multiple scales and the average equations turn to be a Z 2-symmetric perturbed polynomial Hamiltonian system of degree 5. Then, with the bifurcations theory and method of detection function, the qualitative behavior of the unperturbed system and the number of the limit cycles of the perturbed system for certain groups of parameter are analyzed. At last, the stability of the limit cycles is studied and the physical meaning of Tokamak equations under these parameter groups is given. PMID:24892099

Runaway Electrons Modeling and Nanoparticle Plasma Jet Penetration into Tokamak Plasma

NASA Astrophysics Data System (ADS)

Galkin, S. A.; Bogatu, I. N.

2017-10-01

A novel idea to probe runaway electrons (REs) by superfast injection of high velocity nanoparticle plasma jet (NPPJ) from a plasma accelerator needs to be sustained by both RE dynamics modeling and simulation of NPPJ penetration through increasing tokamak magnetic field. We present our recent progress in both areas. RE simulation is based on the model, including Dreicer and ``avalanche'' mechanisms of RE generation, with emphasis on high Zeff effects. The high-density hyper-velocity C60 and BN NPPJ penetration through transversal B-field is conducted with the Hybrid Electro-Magnetic code (HEM-2D) in cylindrical coordinates, with 1/R B-field dependence for both DIII-D and ITER tokamaks. Work is supported in part by US DOE SBIR Grant.

Solenoid-free plasma start-up in spherical tokamaks

NASA Astrophysics Data System (ADS)

Raman, R.; Shevchenko, V. F.

2014-10-01

The central solenoid is an intrinsic part of all present-day tokamaks and most spherical tokamaks. The spherical torus (ST) confinement concept is projected to operate at high toroidal beta and at a high fraction of the non-inductive bootstrap current as required for an efficient reactor system. The use of a conventional solenoid in a ST-based fusion nuclear facility is generally believed to not be a possibility. Solenoid-free plasma start-up is therefore an area of extensive worldwide research activity. Solenoid-free plasma start-up is also relevant to steady-state tokamak operation, as the central transformer coil of a conventional aspect ratio tokamak reactor would be located in a high radiation environment but would be needed only during the initial discharge initiation and current ramp-up phases. Solenoid-free operation also provides greater flexibility in the selection of the aspect ratio and simplifies the reactor design. Plasma start-up methods based on induction from external poloidal field coils, helicity injection and radio frequency current drive have all made substantial progress towards meeting this important need for the ST. Some of these systems will now undergo the final stages of test in a new generation of large STs, which are scheduled to begin operations during the next two years. This paper reviews research to date on methods for inducing the initial start-up current in STs without reliance on the conventional central solenoid.

OMFIT Tokamak Profile Data Fitting and Physics Analysis

DOE PAGES

Logan, N. C.; Grierson, B. A.; Haskey, S. R.; ...

2018-01-22

Here, One Modeling Framework for Integrated Tasks (OMFIT) has been used to develop a consistent tool for interfacing with, mapping, visualizing, and fitting tokamak profile measurements. OMFIT is used to integrate the many diverse diagnostics on multiple tokamak devices into a regular data structure, consistently applying spatial and temporal treatments to each channel of data. Tokamak data are fundamentally time dependent and are treated so from the start, with front-loaded and logic-based manipulations such as filtering based on the identification of edge-localized modes (ELMs) that commonly scatter data. Fitting is general in its approach, and tailorable in its application inmore » order to address physics constraints and handle the multiple spatial and temporal scales involved. Although community standard one-dimensional fitting is supported, including scale length–fitting and fitting polynomial-exponential blends to capture the H-mode pedestal, OMFITprofiles includes two-dimensional (2-D) fitting using bivariate splines or radial basis functions. These 2-D fits produce regular evolutions in time, removing jitter that has historically been smoothed ad hoc in transport applications. Profiles interface directly with a wide variety of models within the OMFIT framework, providing the inputs for TRANSP, kinetic-EFIT 2-D equilibrium, and GPEC three-dimensional equilibrium calculations. he OMFITprofiles tool’s rapid and comprehensive analysis of dynamic plasma profiles thus provides the critical link between raw tokamak data and simulations necessary for physics understanding.« less

OMFIT Tokamak Profile Data Fitting and Physics Analysis

DOE Office of Scientific and Technical Information (OSTI.GOV)

Logan, N. C.; Grierson, B. A.; Haskey, S. R.

Here, One Modeling Framework for Integrated Tasks (OMFIT) has been used to develop a consistent tool for interfacing with, mapping, visualizing, and fitting tokamak profile measurements. OMFIT is used to integrate the many diverse diagnostics on multiple tokamak devices into a regular data structure, consistently applying spatial and temporal treatments to each channel of data. Tokamak data are fundamentally time dependent and are treated so from the start, with front-loaded and logic-based manipulations such as filtering based on the identification of edge-localized modes (ELMs) that commonly scatter data. Fitting is general in its approach, and tailorable in its application inmore » order to address physics constraints and handle the multiple spatial and temporal scales involved. Although community standard one-dimensional fitting is supported, including scale length–fitting and fitting polynomial-exponential blends to capture the H-mode pedestal, OMFITprofiles includes two-dimensional (2-D) fitting using bivariate splines or radial basis functions. These 2-D fits produce regular evolutions in time, removing jitter that has historically been smoothed ad hoc in transport applications. Profiles interface directly with a wide variety of models within the OMFIT framework, providing the inputs for TRANSP, kinetic-EFIT 2-D equilibrium, and GPEC three-dimensional equilibrium calculations. he OMFITprofiles tool’s rapid and comprehensive analysis of dynamic plasma profiles thus provides the critical link between raw tokamak data and simulations necessary for physics understanding.« less

The growth-inhibitory and apoptosis-inducing effect of deferoxamine combined with arsenic trioxide on HL-60 xenografts in nude mice.

PubMed

Yu, Runhong; Wang, Dao; Ren, Xiuhua; Zeng, Li; Liu, Yufeng

2014-09-01

The aim of this study is to investigate the growth-inhibitory and apoptosis-inducing effect of deferoxamine (DFO) combined with arsenic trioxide (ATO) on the human HL-60 xenografts in nude mice and its mechanism. The highly tumorigenic leukemia cell line HL-60 cells were inoculated subcutaneously into nude mice to establish a human leukemia xenograft model. The HL-60 xenograft nude mice models were randomly divided into four groups: control (Normal saline, NS), 50mg/kg DFO, 3mg/kg ATO, the combined treatment (50mg/kg DFO+1.5mg/kg ATO) once HL-60 cells were inoculated. Tumor sizes, growth curves, inhibitory rates, cell apoptosis, and the expression of apoptosis related markers were measured to evaluate the tumor growth. Xenografted tumors were observed in all nude mice since the 5th day of inoculation. The inhibitory rates of tumor weight were 2.67%, 10.69%, and 25.57% in DFO, ATO and combination therapy groups, respectively. The combination of DFO with ATO induces significantly more tumor cell apoptosis than either agent alone (p<0.05). The expression of NF-κBp65 and survivin proteins decreased significantly while the expression of Caspase-3 and Bax increased in the combination therapy group (p<0.05). Double immunofluorescence for Caspase-3 and NFκBp65 demonstrated an inverse relationship between Caspase-3-positive areas and NFκBp65-positive areas, as well as the co-localization of Bax and survivin in xenografted tumor cells. Combination of DFO and ATO has synergistic effects on tumor growth inhibition and apoptosis-inducing in vivo with no significant side effects. The DFO and ATO can up-regulate the expression of Caspase-3 and Bax, and down-regulate the expression of NF-κBp65 and survivin, especially for their combination. Copyright © 2014 Elsevier Ltd. All rights reserved.

Genes encoded within 8q24 on the amplicon of a large extrachromosomal element are selectively repressed during the terminal differentiation of HL-60 cells.

PubMed

Hirano, Tetsuo; Ike, Fumio; Murata, Takehide; Obata, Yuichi; Utiyama, Hiroyasu; Yokoyama, Kazunari K

2008-04-02

Human acute myeloblastic leukemia HL-60 cells become resistant to differentiation during long-term cultivation. After 150 passages, double minute chromosomes (dmins) found in early-passaged cells are replaced by large extrachromosomal elements (LEEs). In a DNA library derived from a purified fraction of LEEs, 12.6% (23/183) of clones were assigned to 8q24 and 9.2% (17/183) were assigned to 14q11 in the human genome. Fluorescence in situ hybridization (FISH) revealed a small aberrant chromosome, which had not been found in early-passaged cells, in addition to the purified LEEs. We determined that each LEE consisted of six discontinuous segments in a region that extended for 4.4Mb over the 8q24 locus. Five genes, namely, Myc (a proto-oncogene), NSMCE2 (for a SUMO ligase), CCDC26 (for a retinoic acid-dependent modulator of myeloid differentiation), TRIB1 (for a regulator of MAPK kinase) and LOC389637 (for a protein of unknown function), were encoded by the amplicon. Breaks in the chromosomal DNA within the amplicon were found in the NSMCE2 and CCDC26 genes. The discontinuous structure of the amplicon unit of the LEEs was identical with that of dmins in HL-60 early-passaged cells. The difference between them seemed, predominantly, to be the number (10-15 copies per LEE versus 2 or 3 copies per dmin) of constituent units. Expression of the Myc, NSMCE2, CCDC26 and LOC389637 and TRIB1 genes was constitutive in all lines of HL-60 cells and that of the first four genes was repressed during the terminal differentiation of early-passaged HL-60 cells. We also detected abnormal transcripts of CCDC26. Our results suggest that these genes were selected during the development of amplicons. They might be amplified and, sometimes, truncated to contribute to the maintenance of HL-60 cells in an undifferentiated state.

Geodesic acoustic modes in noncircular cross section tokamaks

DOE Office of Scientific and Technical Information (OSTI.GOV)

Sorokina, E. A., E-mail: sorokina.ekaterina@gmail.com; Lakhin, V. P.; Konovaltseva, L. V.

2017-03-15

The influence of the shape of the plasma cross section on the continuous spectrum of geodesic acoustic modes (GAMs) in a tokamak is analyzed in the framework of the MHD model. An expression for the frequency of a local GAM for a model noncircular cross section plasma equilibrium is derived. Amendments to the oscillation frequency due to the plasma elongation and triangularity and finite tokamak aspect ratio are calculated. It is shown that the main factor affecting the GAM spectrum is the plasma elongation, resulting in a significant decrease in the mode frequency.

Nearly axisymmetric hot plasmas in a highly rippled tokamak

NASA Astrophysics Data System (ADS)

Bellan, Paul

2002-11-01

Tokamak ohmic heating current flowing along toroidally rippled flux surfaces results in a poloidal torque. Since pressure gradients cannot offset torques, the torque drives plasma flows which convect plasma toroidally from ripple necks (high B_pol^2) to ripple bulges (low B_pol^2). Stagnation of the oppositely directed toroidal flows at the ripple bulges thermalizes the directed flow velocity ˜ B_pol/μ_0ρ , giving β _pol ˜1. These flows also convect frozen-in poloidal field lines which accumulate at the bulges enhancing the pinch force there and so reducing the bulge. Thus, a nearly axisymmetric β_pol ˜1 equilibrium is achieved using only a few TF coils. Particles bouncing in step between approaching flows will be Fermi accelerated to form a high energy tail. The ST tokamak magnetic mountain experiment [1] showed that, compared to a 1.8% ripple configuration, a 28% ripple configuration had four times the neutron production, and only a modest degradation of overall confinement; the former is consistent with the notion of Fermi acceleration of particles bouncing between colliding toroidal flows and the latter is consistent with ripple reduction due to toroidal convection of poloidal field lines. [1] W. Stodiek et al, Proc. 4th Intl. Conf. Plasma Phys. and Contr. Nuc. Fusion Res., (Madison, 1971), Vol. 1, p. 465

The induction of monocytopoiesis in HL-60 promyelocytic leukemia cells is inhibited by hydroquinone, a toxic metabolite of benzene

DOE Office of Scientific and Technical Information (OSTI.GOV)

Oliveira, N.L.

1992-01-01

Chronic exposure of humans to benzene has been shown to have a cytotoxic effect on hematopoietic progenitor cells in intermediate stages of differentiation which can lead to aplastic anemia and acute myelogenous leukemia. This thesis examined the effect of hydroquinone, a toxic metabolite of benzene found in the bone marrow, on the human promyelocytic leukemia cell line (HL-60) which can be induced to differentiate to both monocyte and myeloid cells, and thus has been used as a surrogate for a granulocyte/macrophage progenitor cell. Exposure of HL-60 cells to noncytotoxic concentrations of hydroquinone for three hours prior to induction with 12-O-tetradecanoylmore » phorbol-13-acetate caused a dose-dependent inhibition of the acquisition of characteristics of monocytic differentiation. These included adherence, nonspecific esterase activity and phagocytosis. Hydroquinone had no effect on cell proliferation. Hydroquinone appeared to be affecting maturation beyond the monoblast/promonocyte stages. Hydroquinone also prevented differentiation induced by 1, 25-dihydroxy vitamin D[sub 3], however, the block occurred after the acquisition of adherence. Hydroquinone at concentrations that inhibited monocytic differentiation had no effect on differentiation to granulocytes, suggesting that the block in the differentiation of these bipotential cells is at a step unique to the monocytic pathway. Hydroquinone was unable to prevent differentiation induced by the macrophage-derived cytokine interleukin-1, a differentiation factor for cells of the monocytic lineage. These data demonstrate that treatment of Hl-60 cells with hydroquinone prior to induction of differentiation prevents the acquisition of the monocytic phenotype induced by TPA or 1, 25(OH)[sub 2]D[sub 3] by a mechanism which at present is unknown, but which appears to be specific for the monocytic pathway. These results are of considerable significance for benzene hematotoxicity.« less

Can a Penning ionization discharge simulate the tokamak scrape-off plasma conditions?

NASA Technical Reports Server (NTRS)

Finkenthal, M.; Littman, A.; Stutman, D.; Kovnovich, S.; Mandelbaum, P.; Schwob, J. L.; Bhatia, A. K.

1990-01-01

The tokamak scrape-off (the region between the vacuum vessel wall and the magnetically confined fusion plasma edge), represents a source/sink for the hot fusion plasma. The electron densities and temperatures are in the ranges 10 to the 11th - 10 to the 13th/cu cm and 1-40 eV, respectively (depending on the size, magnetic field intensity and configuration, plasma current, etc). In the work reported, the electron temperature and density have been estimated in a Penning ionization discharge by comparing its spectroscopic emission in the VUV with that predicted by a collisional radiative model. An attempt to directly compare this emission with that of the tokamak edge is briefly described.

A Research Program of Spherical Tokamak in China

NASA Astrophysics Data System (ADS)

He, Ye-xi

2002-08-01

The mission of this program is to explore the spherical torus plasma with a SUNIST spherical tokamak. Main experiments in the start phase will be involved with breakdown and plasma current set-up with a mode of saving volt-second and without ohmic heating system, equilibrium and instability, current driving, heating and profile modification. The SUNIST is a university-scale conceptual spherical tokamak, with R = 0.3 m, A 1.3, Ip ~ 50 kA, BT < 0.15 T, and PRF = 100 kW. The only peculiarity of SUNIST is that there is a toroidal insulating break along the outer wall of vacuum vessel. The expected that advantages of this arrangement are helpful not only for saving flux swing, but also for having a deep understanding of what will influence the discharge startup and globe performances of plasma under different conditions of strong vessel eddy and ECR power assistance. Of course, the vessel structure of cross seal will be at a great risk of controlling vacuum quality, although we have achieved positive results on simulation test and vacuum vessel test.

The Research Progress of the J-TEXT Tokamak

NASA Astrophysics Data System (ADS)

Zhuang, Ge; Wang, Zhijiang; Ding, Yonghua; Zhang, Ming; Yang, Zhoujun; Gao, Li; Zhang, Xiaoqing; Hu, Xiwei; Pan, Yuan

2010-11-01

In 2004, the TEXT-U tokamak was disassembled and shipped to China, and later on settle down in Huazhong University of Science and Technology. The machine was renamed as the Joint TEXT (J-TEXT) tokamak and obtained its first plasma in 2007. The typical J-TEXT Ohmic discharge was performed in the limiter configuration with the main parameters as follows: major radius R=1.05 m, minor radius a=0.27m, toroidal magnetic field BT=2.2T, plasma current Ip>200kA, line-averaged density ne˜ 2-3 . 1019/m^3, and electron temperature Te0˜ 700eV. Up till now, a few diagnostic systems used to facilitate routine operation and experimental studies were designed and developed. Benefiting from these diagnostic tools, the observation of MHD activities and the statistical analysis of disruption events were done. And measurements of the electrostatic fluctuations in the edge region and conditional analysis of the intermittent burst events near the LCFS were also made as well. The preliminary results will be presented in detail in the meeting.

Study of SOL in DIII-D tokamak with SOLPS suite of codes.

NASA Astrophysics Data System (ADS)

Pankin, Alexei; Bateman, Glenn; Brennan, Dylan; Coster, David; Hogan, John; Kritz, Arnold; Kukushkin, Andrey; Schnack, Dalton; Snyder, Phil

2005-10-01

The scrape-of-layer (SOL) region in DIII-D tokamak is studied with the SOLPS integrated suite of codes. The SOLPS package includes the 3D multi-species Monte-Carlo neutral code EIRINE and 2D multi-fluid code B2. The EIRINE and B2 codes are cross-coupled through B2-EIRINE interface. The results of SOLPS simulations are used in the integrated modeling of the plasma edge in DIII-D tokamak with the ASTRA transport code. Parameterized dependences for neutral particle fluxes that are computed with the SOLPS code are implemented in a model for the H-mode pedestal and ELMs [1] in the ASTRA code. The effects of neutrals on the H-mode pedestal and ELMs are studied in this report. [1] A. Y. Pankin, I. Voitsekhovitch, G. Bateman, et al., Plasma Phys. Control. Fusion 47, 483 (2005).

An efficient transport solver for tokamak plasmas

DOE PAGES

Park, Jin Myung; Murakami, Masanori; St. John, H. E.; ...

2017-01-03

A simple approach to efficiently solve a coupled set of 1-D diffusion-type transport equations with a stiff transport model for tokamak plasmas is presented based on the 4th order accurate Interpolated Differential Operator scheme along with a nonlinear iteration method derived from a root-finding algorithm. Here, numerical tests using the Trapped Gyro-Landau-Fluid model show that the presented high order method provides an accurate transport solution using a small number of grid points with robust nonlinear convergence.

HL-20 structural design comparison - Conformal shell versus cylindrical crew compartment

NASA Technical Reports Server (NTRS)

Bush, Lance B.; Wahls, Deborah M.; Robinson, James C.

1993-01-01

Extensive studies have been performed at NASA Langley Research Center (LaRC) on personnel launch systems (PLS) concepts. The primary mission of a PLS is the transport of Space Station crew members from Earth to the Space Station and return. The NASA LaRC PLS studies have led to the design of a lifting body configuration named the HL-20. In this study, two different HL-20 structural configurations are evaluated. The two configurations are deemed the conformal shell and the cylindrical crew compartment. The configurations are based on two different concerns for maintenance and operations. One configuration allows for access to subsystems while on-orbit from the interior, while the other allows for easy access to the subsystems during ground maintenance and operations. For each concept, the total structural weight required to sustain the applied loads is quantified through a structural evaluation. Structural weight for both configurations is compared along with the particular attributes of each. Analyses of both configurations indicate no appreciable weight or load relief advantage of one concept over the other. Maintainability and operability, therefore become the primary discriminator, leading to a choice of a crew compartment configuration.

Capillin, a major constituent of Artemisia capillaris Thunb. flower essential oil, induces apoptosis through the mitochondrial pathway in human leukemia HL-60 cells.

PubMed

Masuda, Yutaka; Asada, Keisuke; Satoh, Rei; Takada, Kimihiko; Kitajima, Junichi

2015-05-15

Natural products are one of the most important sources of drugs used in pharmaceutical therapeutics. Screening of several natural products in the search for novel anticancer agents against human leukemia HL-60 cells led us to identify potent apoptosis-inducing activity in the essential oil fraction from Artemisia capillaris Thunb. flower. The cytotoxic effects of extracts were assessed on human leukemia HL-60 cells by XTT assay. Induction of apoptosis was assessed by analysis of DNA fragmentation and nuclear morphological change. The plant name was checked with the plant list website (http://www.theplantlist.org). A purified compound from the essential oil fraction from Artemisia capillaris Thunb. flower that potently inhibited cell growth in human leukemia HL-60 cells was identified as capillin. The cytotoxic effect of capillin in cells was associated with apoptosis. When HL-60 cells were treated with 10(-6) M capillin for 6 h, characteristic features of apoptosis such as DNA fragmentation and nuclear fragmentation were observed. Moreover, activation of c-Jun N-terminal kinase (JNK) was detected after treatment with capillin preceding the appearance of characteristic properties of apoptosis. Release of cytochrome c from mitochondria was also observed in HL-60 cells that had been treated with capillin. Capillin induces apoptosis in HL-60 cells via the mitochondrial apoptotic pathway, which might be controlled through JNK signaling. Our results indicate that capillin may be a potentially useful anticancer drug that could enhance therapeutic efficacy. Copyright © 2015 Elsevier GmbH. All rights reserved.

The development of MML (Medical Markup Language) version 3.0 as a medical document exchange format for HL7 messages.

PubMed

Guo, Jinqiu; Takada, Akira; Tanaka, Koji; Sato, Junzo; Suzuki, Muneou; Suzuki, Toshiaki; Nakashima, Yusei; Araki, Kenji; Yoshihara, Hiroyuki

2004-12-01

Medical Markup Language (MML), as a set of standards, has been developed over the last 8 years to allow the exchange of medical data between different medical information providers. MML Version 2.21 used XML as a metalanguage and was announced in 1999. In 2001, MML was updated to Version 2.3, which contained 12 modules. The latest version--Version 3.0--is based on the HL7 Clinical Document Architecture (CDA). During the development of this new version, the structure of MML Version 2.3 was analyzed, subdivided into several categories, and redefined so the information defined in MML could be described in HL7 CDA Level One. As a result of this development, it has become possible to exchange MML Version 3.0 medical documents via HL7 messages.

Conceptual design study of the moderate size superconducting spherical tokamak power plant

NASA Astrophysics Data System (ADS)

Gi, Keii; Ono, Yasushi; Nakamura, Makoto; Someya, Youji; Utoh, Hiroyasu; Tobita, Kenji; Ono, Masayuki

2015-06-01

A new conceptual design of the superconducting spherical tokamak (ST) power plant was proposed as an attractive choice for tokamak fusion reactors. We reassessed a possibility of the ST as a power plant using the conservative reactor engineering constraints often used for the conventional tokamak reactor design. An extensive parameters scan which covers all ranges of feasible superconducting ST reactors was completed, and five constraints which include already achieved plasma magnetohydrodynamic (MHD) and confinement parameters in ST experiments were established for the purpose of choosing the optimum operation point. Based on comparison with the estimated future energy costs of electricity (COEs) in Japan, cost-effective ST reactors can be designed if their COEs are smaller than 120 mills kW-1 h-1 (2013). We selected the optimized design point: A = 2.0 and Rp = 5.4 m after considering the maintenance scheme and TF ripple. A self-consistent free-boundary MHD equilibrium and poloidal field coil configuration of the ST reactor were designed by modifying the neutral beam injection system and plasma profiles. The MHD stability of the equilibrium was analysed and a ramp-up scenario was considered for ensuring the new ST design. The optimized moderate-size ST power plant conceptual design realizes realistic plasma and fusion engineering parameters keeping its economic competitiveness against existing energy sources in Japan.

ECRH Studies on Tokamak Plasmas.

DTIC Science & Technology

1980-10-10

r.I*cru.Dtrtibution uUnliited 300 Unicorn Pork Drive Woburn, Massachusetts 04801 ECRH STUDIES ON TOKAMAK PLASMAS JAYCOR Project No. 6183 Final Report...up techniques now in use or being suggested, include growing the plasma from a small minor radius or applying a negative voltage spike immediately

Progress toward steady-state tokamak operation exploiting the high bootstrap current fraction regime

DOE PAGES

Ren, Q. L.; Garofalo, A. M.; Gong, X. Z.; ...

2016-06-20

Recent DIII-D experiments have increased the normalized fusion performance of the high bootstrap current fraction tokamak regime toward reactor-relevant steady state operation. The experiments, conducted by a joint team of researchers from the DIII-D and EAST tokamaks, developed a fully noninductive scenario that could be extended on EAST to a demonstration of long pulse steady-state tokamak operation. Improved understanding of scenario stability has led to the achievement of very high values of β p and β N despite strong ITBs. Good confinement has been achieved with reduced toroidal rotation. These high β p plasmas challenge the energy transport understanding, especiallymore » in the electron energy channel. A new turbulent transport model, named 2 TGLF-SAT1, has been developed which improves the transport prediction. Experiments extending results to long pulse on EAST, based on the physics basis developed at DIII-D, have been conducted. Finally, more investigations will be carried out on EAST with more additional auxiliary power to come online in the near term.« less

Copper(II) complexes with 4-(1H-1, 2, 4-trizol-1-ylmethyl) benzoic acid: Syntheses, crystal structures and antifungal activities

NASA Astrophysics Data System (ADS)

Xiong, Pingping; Li, Jie; Bu, Huaiyu; Wei, Qing; Zhang, Ruolin; Chen, Sanping

2014-07-01

Reaction of Cu(II) with an asymmetric semi-rigid organic ligand 4-(1H-1, 2, 4-trizol-1-ylmethyl) benzoic acid (HL), yielded five compounds, [Cu0.5L]n (1), [Cu(HL)2Cl2]n (2), [Cu(HL)2Cl2(H2O)] (3), [Cu(L)2(H2O)]n (4) and [Cu(L)(phen)(HCO2)]n (5), which have been fully characterized by infrared spectroscopy, elemental analysis, and single-crystal X-ray diffraction. As for compounds 1, 2 and 5, Cu(II) is bridged through HL, Cl-, and formic acid, respectively, featuring 1D chain-structure. In compound 3, Cu(II) with hexahedral coordination sphere is assembled through hydrogen-bonding into 3D supramolecular framework. In compound 4, 1D chain units -Cu-O-Cu-O- are ligand-bridged into a 3D network. All compounds were tested on fungi (Fusarium graminearum, Altemaria solani, Macrophoma kawatsukai, Alternaria alternata and Colletotrichum gloeosporioides). Compound 1 exhibits a better antifungal effect compared to other compounds. An effect of structure on the antifungal activity has also been correlated.

Design, simulation and construction of the Taban tokamak

NASA Astrophysics Data System (ADS)

H, R. MIRZAEI; R, AMROLLAHI

2018-04-01

This paper describes the design and construction of the Taban tokamak, which is located in Amirkabir University of Technology, Tehran, Iran. The Taban tokamak was designed for plasma investigation. The design, simulation and construction of essential parts of the Taban tokamak such as the toroidal field (TF) system, ohmic heating (OH) system and equilibrium field system and their power supplies are presented. For the Taban tokamak, the toroidal magnetic coil was designed to produce a maximum field of 0.7 T at R = 0.45 m. The power supply of the TF was a 130 kJ, 0–10 kV capacitor bank. Ripples of toroidal magnetic field at the plasma edge and plasma center are 0.2% and 0.014%, respectively. For the OH system with 3 kA current, the stray field in the plasma region is less than 40 G over 80% of the plasma volume. The power supply of the OH system consists of two stages, as follows. The fast bank stage is a 120 μF, 0–5 kV capacitor that produces 2.5 kA in 400 μs and the slow bank stage is 93 mF, 600 V that can produce a maximum of 3 kA. The equilibrium system can produce uniform magnetic field at plasma volume. This system’s power supply, like the OH system, consists of two stages, so that the fast bank stage is 500 μF, 800 V and the slow bank stage is 110 mF, 200 V.

Plasma Equilibrium Control in Nuclear Fusion Devices 2. Plasma Control in Magnetic Confinement Devices 2.1 Plasma Control in Tokamaks

NASA Astrophysics Data System (ADS)

Fukuda, Takeshi

The plasma control technique for use in large tokamak devices has made great developmental strides in the last decade, concomitantly with progress in the understanding of tokamak physics and in part facilitated by the substantial advancement in the computing environment. Equilibrium control procedures have thereby been established, and it has been pervasively recognized in recent years that the real-time feedback control of physical quantities is indispensable for the improvement and sustainment of plasma performance in a quasi-steady-state. Further development is presently undertaken to realize the “advanced plasma control” concept, where integrated fusion performance is achieved by the simultaneous feedback control of multiple physical quantities, combined with equilibrium control.

Aerodynamic heating environment definition/thermal protection system selection for the HL-20

NASA Astrophysics Data System (ADS)

Wurster, K. E.; Stone, H. W.

1993-09-01

Definition of the aerothermal environment is critical to any vehicle such as the HL-20 Personnel Launch System that operates within the hypersonic flight regime. Selection of an appropriate thermal protection system design is highly dependent on the accuracy of the heating-environment prediction. It is demonstrated that the entry environment determines the thermal protection system design for this vehicle. The methods used to predict the thermal environment for the HL-20 Personnel Launch System vehicle are described. Comparisons of the engineering solutions with computational fluid dynamic predictions, as well as wind-tunnel test results, show good agreement. The aeroheating predictions over several critical regions of the vehicle, including the stagnation areas of the nose and leading edges, windward centerline and wing surfaces, and leeward surfaces, are discussed. Results of predictions based on the engineering methods found within the MINIVER aerodynamic heating code are used in conjunction with the results of the extensive wind-tunnel tests on this configuration to define a flight thermal environment. Finally, the selection of the thermal protection system based on these predictions and current technology is described.

Feedback-Assisted Extension of the Tokamak Operating Space to Low Safety Factor

NASA Astrophysics Data System (ADS)

Hanson, J. M.

2013-10-01

Recent DIII-D experiments have demonstrated stable operation at very low edge safety factor, q95 <~ 2 through the use of magnetic feedback to control the n = 1 resistive wall mode (RWM) instability. The performance of tokamak fusion devices may benefit from increased plasma current, and thus, decreased q. However, disruptive stability limits are commonly encountered in experiments at qedge ~ 2 (limited plasmas) and q95 ~ 2 (diverted plasmas), limiting exploration of low q regimes. In the recent DIII-D experiments, the impact and control of key disruptive instabilities was studied. Locked n = 1 modes with exponential growth times on the order of the wall eddy current decay timescale τw preceded disruptions at q95 = 2 . The instabilities have a poloidal structure that is consistent with VALEN simulations of the RWM mode structure at q95 = 2 . Applying proportional gain magnetic feedback control of the n = 1 mode resulted in stabilized operation with q95 reaching 1.9, and an extension of the discharge lifetime for > 100τw . Loss of feedback control was accompanied by power supply saturation, followed by a rapidly growing n = 1 mode and disruption. Comparisons of the feedback dynamics with VALEN simulations will be presented. The DIII-D results complement and will be discussed alongside recent RFX-MOD demonstrations of RWM control using magnetic feedback in limited tokamak discharges with qedge < 2. These results call attention to the utility of magnetic feedback in significantly extending the tokamak operational space and potentially opening a new route to economical fusion power production. Supported by the US Department of Energy under DE-FG02-04ER54761 and DE-FC02-04ER54698.

Numerical modelling of geodesic acoustic mode relaxation in a tokamak edge

DOE PAGES

Dorf, M. A.; Cohen, R. H.; Dorr, M.; ...

2013-05-08

Here, the edge of a tokamak in a high confinement (H mode) regime is characterized by steep density gradients and a large radial electric field. Recent analytical studies demonstrated that the presence of a strong radial electric field consistent with a subsonic pedestal equilibrium modifies the conventional results of the neoclassical formalism developed for the core region. In the present work we make use of the recently developed gyrokinetic code COGENT to numerically investigate neoclassical transport in a tokamak edge including the effects of a strong radial electric field. The results of numerical simulations are found to be in goodmore » qualitative agreement with the theoretical predictions and the quantitative discrepancy is discussed. In addition, the present work investigates the effects of a strong radial electric field on the relaxation of geodesic acoustic modes (GAMs) in a tokamak edge. Numerical simulations demonstrate that the presence of a strong radial electric field characteristic of a tokamak pedestal can enhance the GAM decay rate, and heuristic arguments elucidating this finding are provided.« less

Fishbone activity in experimental advanced superconducting tokamak neutral beam injection plasma

DOE Office of Scientific and Technical Information (OSTI.GOV)

Xu, Liqing; Zhang, Jizong; Chen, Kaiyun, E-mail: Kychen@ipp.cas.cn, E-mail: lqhu@ipp.cas.cn

2015-12-15

Repetitive fishbones near the trapped ion procession frequency were observed for the first time in the neutral beam injection high confinement plasmas in Experimental Advanced Superconducting Tokamak (EAST) tokamak, and diagnosed using a solid-state neutral particle analyzer based on a compact silicon photodiode together with an upgraded high spatial-temporal-resolution multi-arrays soft X-ray (SX) system. This 1/1 typical internal kink mode propagates in the ion-diamagnetism direction with a rotation speed faster than the bulk plasma in the plasma frame. From the SX measurements, this mode frequency is typical of chirping down and the energetic particle effect related to the twisting modemore » structure. This ion fishbone was found able to trigger a multiple core sawtooth crashes with edge-2/1 sideband modes, as well as to lead to a transition from fishbone to long lived saturated kink mode to fishbone. Furthermore, using SX tomography, a correlation between mode amplitude and mode frequency was found. Finally, a phenomenological prey–predator model was found to reproduce the fishbone nonlinear process well.« less

Neutral-beam deposition in large, finite-beta noncircular tokamak plasmas

DOE Office of Scientific and Technical Information (OSTI.GOV)

Wieland, R.M.; Houlberg, W.A.

1982-02-01

A parametric pencil beam model is introduced for describing the attenuation of an energetic neutral beam moving through a tokamak plasma. The nonnegligible effects of a finite beam cross section and noncircular shifted plasma cross sections are accounted for in a simple way by using a smoothing algorithm dependent linearly on beam radius and by including information on the plasma flux surface geometry explicitly. The model is benchmarked against more complete and more time-consuming two-dimensional Monte Carlo calculations for the case of a large D-shaped tokamak plasma with minor radius a = 120 cm and elongation b/a = 1.6. Depositionmore » profiles are compared for deuterium beam energies of 120 to 150 keV, central plasma densities of 8 x 10/sup 13/ - 2 x 10/sup 14/ cm/sup -3/, and beam orientation ranging from perpendicular to tangential to the inside wall.« less

Amino acid sequences of the ribosomal proteins HL30 and HmaL5 from the archaebacterium Halobacterium marismortui.

PubMed

Hatakeyama, T; Hatakeyama, T

1990-07-06

The complete amino acid sequences of the ribosomal proteins HL30 and HmaL5 from the archaebacterium Halobacterium marismortui were determined. Protein HL30 was found to be acetylated at its N-terminal amino acid and shows homology to the eukaryotic ribosomal proteins YL34 from yeast and RL31 from rat. Protein HmaL5 was homologous to the protein L5 from Escherichia coli and Bacillus stearothermophilus as well as to YL16 from yeast. HmaL5 shows more similarities to its eukaryotic counterpart than to eubacterial ones.

Compact Torus Injection Experiments on the H.I.T. teststand and the JFT-2M tokamak

NASA Astrophysics Data System (ADS)

Fukumoto, Naoyuki; Fujiwara, Makoto; Kuramoto, Keiji; Ageishi, Masaya; Nagata, Masayoshi; Uyama, Tadao; Ogawa, Hiroaki; Kasai, Satoshi; Hasegawa, Kouichi; Shibata, Takatoshi

1997-11-01

A spheromak-type compact torus (CT) acceleration and injection experiment has been carried out using the Himeji Institute of Technology Compact Torus Injector (HIT-CTI). We investigate the possibility of refueling, density control, current drive, and edge electric field control of tokamak plasmas by means of CT injection. The HIT-CTI produces a CT with a speed of 200 km/s and a density of 1× 10^21m-3. We have constructed new electrodes and power supplies, and will install the HIT-CTI on the JFT-2M tokamak at JAERI in Autumn 1997. The outer electrode serves as a common ground for both the formation bank (144μF, 20kV) and the acceleration bank (92.4μF, 40kV). If the external toroidal field of the tokamak is applied across the CT acceleration region, the CT kinetic energy might decrease during penetration into the field lines joining the inner and outer electrode. This could result in the CT not being able to reach the core of the tokamak plasma. Determining the optimum position of the inner electrode is one of the near term goals of this research. We will present magnetic probe, He-Ne interferometer and fast framing camera data from experiments at H.I.T., where a CT was accelerated into a transverse field. We will also present initial results from the operation of the HIT-CTI on the JFT-2M tokamak.

Modulation of ionizing radiation-induced apoptosis and cell cycle arrest by all-trans retinoic acid in promyelocytic leukemia cells (HL-60).

PubMed

Mareková, M; Vávrová, J; Vokurková, D; Psutka, J

2003-01-01

Acute promyelocytic leukemia is characterized by a block of myeloid differentiation. The incubation of cells with 1 micromol/l all-trans retinoic acid (ATRA) for 72 h induced differentiation of HL-60 cells and increased the number of CD11b positive cells. Morphological and functional changes were accompanied by a loss of proliferative capacity. Differentiation caused by preincubation of leukemic cells HL-60 with ATRA is accompanied by loss of clonogenicity (control cells: 870 colonies/10(3) cells, cells preincubated with ATRA: 150 colonies/10(3) cells). D0 for undifferentiated cells was 2.35 Gy, for ATRA differentiated cells 2.46 Gy. Statistical comparison of clonogenity curves indicated that in the whole range 0.5-10 Gy the curves are not significantly different, however, in the range 0.5-3 Gy ATRA differentiated cells were significantly more radioresistant than non-differentiated cells. When HL-60 cells preincubated with 1 micromol/l ATRA were irradiated by a sublethal dose of 6 Gy, more marked increase of apoptotic cells number was observed 24 h after irradiation and the surviving cells were mainly in the G1 phase of the cell cycle, while only irradiated cells were accumulated in G(2) phase. Our results imply that preincubation of cells with ATRA accelerates apoptosis occurrence (24 h) after irradiation by high sublethal dose of 6 Gy. Forty-eight hours after 6 Gy irradiation, late apoptotic cells were found in the group of ATRA pretreated cells, as determined by APO2.7 positivity. This test showed an increased effect (considering cell death induction) in comparison to ATRA or irradiation itself.

Fibroblast growth factor-2 (FGF2) and syndecan-1 (SDC1) are potential biomarkers for putative circulating CD15+/CD30+ cells in poor outcome Hodgkin lymphoma patients

PubMed Central

2013-01-01

Background High risk, unfavorable classical Hodgkin lymphoma (cHL) includes those patients with primary refractory or early relapse, and progressive disease. To improve the availability of biomarkers for this group of patients, we investigated both tumor biopsies and peripheral blood leukocytes (PBL) of untreated (chemo-naïve, CN) Nodular Sclerosis Classic Hodgkin Lymphoma (NS-cHL) patients for consistent biomarkers that can predict the outcome prior to frontline treatment. Methods and materials Bioinformatics data mining was used to generate 151 candidate biomarkers, which were screened against a library of 10 HL cell lines. Expression of FGF2 and SDC1 by CD30+ cells from HL patient samples representing good and poor outcomes were analyzed by qRT-PCR, immunohistochemical (IHC), and immunofluorescence analyses. Results To identify predictive HL-specific biomarkers, potential marker genes selected using bioinformatics approaches were screened against HL cell lines and HL patient samples. Fibroblast Growth Factor-2 (FGF2) and Syndecan-1 (SDC1) were overexpressed in all HL cell lines, and the overexpression was HL-specific when compared to 116 non-Hodgkin lymphoma tissues. In the analysis of stratified NS-cHL patient samples, expression of FGF2 and SDC1 were 245 fold and 91 fold higher, respectively, in the poor outcome (PO) group than in the good outcome (GO) group. The PO group exhibited higher expression of the HL marker CD30, the macrophage marker CD68, and metastatic markers TGFβ1 and MMP9 compared to the GO group. This expression signature was confirmed by qualitative immunohistochemical and immunofluorescent data. A Kaplan-Meier analysis indicated that samples in which the CD30+ cells carried an FGF2+/SDC1+ immunophenotype showed shortened survival. Analysis of chemo-naive HL blood samples suggested that in the PO group a subset of CD30+ HL cells had entered the circulation. These cells significantly overexpressed FGF2 and SDC1 compared to the GO group. The

A polysaccharide from Sanguisorbae radix induces caspase-dependent apoptosis in human leukemia HL-60 cells.

PubMed

Wu, Zhigang; Sun, Honghui; Li, Jingzhong; Ma, Chijiao; Zhao, Siqiao; Guo, Zheng; Lin, Yao; Lin, Yaping; Liu, Li

2014-09-01

One polysaccharide (SRP) was purified from Sanguisorbae radix by DEAE-cellulose-52 anion-exchange and Sephacryl S-400 gel filtration chromatography. The aim of this study was to evaluate the anticancer efficacy of SRP on human leukemia HL-60 cells in vitro and unveil the underlying mechanisms. Our results showed that SRP was able to suppress the proliferation of HL-60 cells in a dose-dependent manner by the mechanism involved in the induction of apoptosis. The increase in SRP-induced apoptosis was correlated with a rapid and sustained loss of mitochondrial transmembrane potential (ΔΨm) and a release of cytochrome c from the mitochondria into the cytosol. Furthermore, Western blot and RT-PCR analysis revealed that the protein and mRNA levels of antiapoptotic Bcl-2 were downregulated, whereas those of pro-apoptotic Bax were upregulated. Besides, caspase-9 and caspase-3 were activated, while caspase-8 was intact. Additionally, the apoptotic cells by SRP were significantly inhibited by a caspase-3 inhibitor (z-DEVD-fmk) or a caspase-9 inhibitor (Z-LETD-FMK), demonstrating the important role of caspase-9 and -3 in the process. Taken together, these findings provided evidence that SRP induced the apoptosis of HL-60 cells through an intrinsic mitochondria-mediated signaling pathway and SRP may be a promising chemotherapeutic agent for treatment of leukemia. Copyright © 2014 Elsevier B.V. All rights reserved.

A symplectic map for trajectories of magnetic field lines in double-null divertor tokamaks

NASA Astrophysics Data System (ADS)

Crank, Willie; Ali, Halima; Punjabi, Alkesh

2009-11-01

The coordinates of the area-preserving map equations for integration of magnetic field line trajectories in tokamaks can be any coordinates for which a transformation to (ψ,θ,φ) coordinates exists [A. Punjabi, H. Ali, T. Evans, and A. Boozer, Phys. Lett. A 364, 140 (2007)]. ψ is toroidal magnetic flux, θ is poloidal angle, and φ is toroidal angle. This freedom is exploited to construct a map that represents the magnetic topology of double-null divertor tokamaks. For this purpose, the generating function of the simple map [A. Punjabi, A. Verma, and A. Boozer, Phys. Rev. Lett. 69, 3322 (1992)] is slightly modified. The resulting map equations for the double-null divertor tokamaks are: x1=x0-ky0(1-y0^2 ), y1=y0+kx1. k is the map parameter. It represents the generic topological effects of toroidal asymmetries. The O-point is at (0.0). The X-points are at (0,±1). The equilibrium magnetic surfaces are calculated. These surfaces are symmetric about the x- and y- axes. The widths of stochastic layer near the X-points in the principal plane, and the fractal dimensions of the magnetic footprints on the inboard and outboard side of upper and lower X-points are calculated from the map. This work is supported by US Department of Energy grants DE-FG02-07ER54937, DE-FG02-01ER54624 and DE-FG02-04ER54793.

High-Luminosity Large Hadron Collider (HL-LHC) : Preliminary Design Report

DOE Office of Scientific and Technical Information (OSTI.GOV)

Apollinari, G.; Béjar Alonso, I.; Brüning, O.

2015-12-17

The Large Hadron Collider (LHC) is one of the largest scientific instruments ever built. Since opening up a new energy frontier for exploration in 2010, it has gathered a global user community of about 7,000 scientists working in fundamental particle physics and the physics of hadronic matter at extreme temperature and density. To sustain and extend its discovery potential, the LHC will need a major upgrade in the 2020s. This will increase its luminosity (rate of collisions) by a factor of five beyond the original design value and the integrated luminosity (total collisions created) by a factor ten. The LHCmore » is already a highly complex and exquisitely optimised machine so this upgrade must be carefully conceived and will require about ten years to implement. The new configuration, known as High Luminosity LHC (HL-LHC), will rely on a number of key innovations that push accelerator technology beyond its present limits. Among these are cutting-edge 11-12 tesla superconducting magnets, compact superconducting cavities for beam rotation with ultra-precise phase control, new technology and physical processes for beam collimation and 300 metre-long high-power superconducting links with negligible energy dissipation. The present document describes the technologies and components that will be used to realise the project and is intended to serve as the basis for the detailed engineering design of HL-LHC.« less

Advanced Divertor Design and Application under Modern Superconducting Tokamak Constraints

NASA Astrophysics Data System (ADS)

Covele, Brent; Kotschenreuther, Mike; Mahajan, Swadesh; Valanju, Prashant

2013-10-01

With current ITER projections already predicting divertor exhaust heat loads in the 5-10 MW/m2 range, i.e. at the maximum tolerance, it is clear that the divertor heat load problem will only be exacerbated for future superconducting tokamaks, as well as perhaps some modern tokamaks today. Thus, an advanced divertor, such as the X-Divertor (XD), Super-X Divertor (SXD), or Snowflake (SF) will become a virtual necessity to reduce incident heat flux at the target plates. Using the 2D magnetic equilibrium code CORSICA, we explore the possibilities of creating an advanced divertor for a next-generation superconducting tokamak (Ip = 15 MA, BT = 5.3 T, R = 6.2 m) under nominal engineering constraints. Advanced divertors were achieved with no in-vessel PF coils, PF current densities below 30 MA/m2, and vertical maintenance access, all of which are favorable conditions for tokamaks today. Both the XD and SF divertors are readily achievable while maintaining core plasma performance, and the advantages and disadvantages of each are discussed in turn. Some thought is given as to how the divertor cassette will need to be modified to accommodate advanced divertors. Work supported under US-DOE projects DE-FG02-04ER54742 and DE-FG02-04ER54754.

Oligomers, protofibrils and amyloid fibrils from recombinant human lysozyme (rHL): fibrillation process and cytotoxicity evaluation for ARPE-19 cell line.

PubMed

Ruiz, Eva D; Almada, Mario; Burboa, María G; Taboada, Pablo; Mosquera, Víctor; Valdez, Miguel A; Juárez, Josué

2015-02-01

Amyloid-associated diseases, such Alzheimer's, Huntington's, Parkinson's, and type II diabetes, are related to protein misfolding and aggregation. Herein, the time evolution of scattered light intensity, hydrophobic properties, and conformational changes during fibrillation processes of rHL solutions at 55 °C and pH 2.0 were used to monitor the aggregation process of recombinant human lysozyme (rHL). Dynamic light scattering (DLS), thioflavin T (ThT) fluorescence, and surface tension (ST) at the air-water interface were used to analyze the hydrophobic properties of pre-amyloid aggregates involved in the fibrillation process of rHL to find a correlation between the hydrophobic character of oligomers, protofibrils and amyloid aggregates with the gain in cross-β-sheet structure, depending on the increase in the incubation periods. The ability of the different aggregates of rHL isolated during the fibrillation process to be adsorbed at the air-water interface can provide important information about the hydrophobic properties of the protein, which can be related to changes in the secondary structure of rHL, resulting in cytotoxic or non-cytotoxic species. Thus, we evaluated the cytotoxic effect of oligomers, protofibrils and amyloid fibrils on the cell line ARPE-19 using the MTT reduction test. The more cytotoxic protein species arose after a 600-min incubation time, suggesting that the hydrophobic character of pre-amyloid fibrils, in addition to the high prevalence of the cross-β-sheet conformation, can become toxic for the cell line ARPE-19. Copyright © 2014 Elsevier B.V. All rights reserved.

Modular coils and finite-β operation of a quasi-axially symmetric tokamak

NASA Astrophysics Data System (ADS)

Drevlak, M.

1998-09-01

Quasi-axially symmetric tokamaks (QA tokamaks) are an extension of the conventional tokamak concept. In these devices the magnetic field strength is independent of the generalized toroidal magnetic co-ordinate even though the cross-sectional shape changes. An optimized plasma equilibrium belonging to the class of QA tokamaks has been proposed by Nührenberg. It features the small aspect ratio of a tokamak while allowing part of the rotational transform to be generated by the external field. In this article, two particular aspects of the viability of QA tokamaks are explored, namely the feasibility of modular coils and the possibility of maintaining quasi-axial symmetry in the free-boundary equilibria obtained with the coils found. A set of easily feasible modular coils for the configuration is presented. It was designed using the extended version of the NESCOIL code (Merkel, P., Nucl. Fusion 27 (1987) 867). Using this coil system, free-boundary calculations of the plasma equilibrium were carried out using the NEMEC code (Hirshman, S.P., Van Rij, W.I., Merkel, P., Comput. Phys. Commun. 43 (1986) 143). It is observed that the effects of finite β and net toroidal plasma current can be compensated for with good precision by applying a vertical magnetic field and by separately adjusting the currents of the modular coils. A set of fully three dimensional (3-D) auxiliary coils is proposed to exert control on the rotational transform in the plasma. Deterioration of the quasi-axial symmetry induced by the auxiliary coils can be avoided by adequate adjustment of the currents in the primary coils. Finally, the neoclassical transport properties of the configuration are examined. It is observed that optimization with respect to confinement of the alpha particles can be maintained at operation with finite toroidal current if the aforementioned corrective measures are used. In this case, the neoclassical behaviour is shown to be very similar to that of a conventional tokamak.

Expression of toll-like receptors 2 and 4 and CD14 during differentiation of HL-60 cells induced by phorbol 12-myristate 13-acetate and 1 alpha, 25-dihydroxy-vitamin D(3).

PubMed

Li, Changlin; Wang, Yibing; Gao, Li; Zhang, Jingsong; Shao, Jie; Wang, Shengnian; Feng, Weiguo; Wang, Xingyu; Li, Minglie; Chang, Zongliang

2002-01-01

Macrophages form a crucial bridge between the innate and adaptive immune response. One of their most important functions is to recognize infectious microorganisms. Toll-like receptors (TLRs) are key elements in pathogen recognition, and among them, TLR2 and TLR4 are most discussed. However, expression patterns of TLRs during myeloid cell differentiation to macrophage are unknown. In this study, we examined differentiation in the model human myeloid cell line, HL-60, treated with phorbol 12-myristate 13-acetate (PMA) or VitD(3). Expression of TLR2, TLR4, and CD14 were measured by reverse transcription-PCR, RNase protection assay, and fluorescence-activated cell sorter assays. After treatment by PMA (1, 10, and 100 nM) for 12, 24, and 48 h, expression of TLR2 and CD14 mRNA was increased in a time- and dose-dependent manner. However, VitD(3) only induced expression of CD14 but not TLR2 in HL-60 cells. TLR4 was expressed constitutively before differentiation and increased slightly after that. Thus, PMA-mediated differentiation of HL-60 cells to macrophages is associated largely with TLR2 expression and, to a much lesser extent, with TLR4. Furthermore, up-regulation of TLR2 and CD14 mRNA expression by PMA was abrogated by a protein kinase C inhibitor, Calphostine C, suggesting the up-regulation of TLR2 and CD14 mRNA is dependent on the activation of protein kinase C. Coexpression of CD14/TLR2 and/or CD14/TLR4 may be essential but not sufficient for the production of tumor necrosis factor-alpha in response to lipopolysaccharide in our system.

Dust measurements in tokamaks (invited).

PubMed

Rudakov, D L; Yu, J H; Boedo, J A; Hollmann, E M; Krasheninnikov, S I; Moyer, R A; Muller, S H; Pigarov, A Yu; Rosenberg, M; Smirnov, R D; West, W P; Boivin, R L; Bray, B D; Brooks, N H; Hyatt, A W; Wong, C P C; Roquemore, A L; Skinner, C H; Solomon, W M; Ratynskaia, S; Fenstermacher, M E; Groth, M; Lasnier, C J; McLean, A G; Stangeby, P C

2008-10-01

Dust production and accumulation present potential safety and operational issues for the ITER. Dust diagnostics can be divided into two groups: diagnostics of dust on surfaces and diagnostics of dust in plasma. Diagnostics from both groups are employed in contemporary tokamaks; new diagnostics suitable for ITER are also being developed and tested. Dust accumulation in ITER is likely to occur in hidden areas, e.g., between tiles and under divertor baffles. A novel electrostatic dust detector for monitoring dust in these regions has been developed and tested at PPPL. In the DIII-D tokamak dust diagnostics include Mie scattering from Nd:YAG lasers, visible imaging, and spectroscopy. Laser scattering is able to resolve particles between 0.16 and 1.6 microm in diameter; using these data the total dust content in the edge plasmas and trends in the dust production rates within this size range have been established. Individual dust particles are observed by visible imaging using fast framing cameras, detecting dust particles of a few microns in diameter and larger. Dust velocities and trajectories can be determined in two-dimension with a single camera or three-dimension using multiple cameras, but determination of particle size is challenging. In order to calibrate diagnostics and benchmark dust dynamics modeling, precharacterized carbon dust has been injected into the lower divertor of DIII-D. Injected dust is seen by cameras, and spectroscopic diagnostics observe an increase in carbon line (CI, CII, C(2) dimer) and thermal continuum emissions from the injected dust. The latter observation can be used in the design of novel dust survey diagnostics.

Quantitative Analysis of Global Proteome and Lysine Acetylome Reveal the Differential Impacts of VPA and SAHA on HL60 Cells.

PubMed

Zhu, Xiaoyu; Liu, Xin; Cheng, Zhongyi; Zhu, Jun; Xu, Lei; Wang, Fengsong; Qi, Wulin; Yan, Jiawei; Liu, Ning; Sun, Zimin; Liu, Huilan; Peng, Xiaojun; Hao, Yingchan; Zheng, Nan; Wu, Quan

2016-01-29

Valproic acid (VPA) and suberoylanilide hydroxamic acid (SAHA) are both HDAC inhibitors (HDACi). Previous studies indicated that both inhibitors show therapeutic effects on acute myeloid leukaemia (AML), while the differential impacts of the two different HDACi on AML treatment still remains elusive. In this study, using 3-plex SILAC based quantitative proteomics technique, anti-acetyllysine antibody based affinity enrichment, high resolution LC-MS/MS and intensive bioinformatic analysis, the quantitative proteome and acetylome in SAHA and VPA treated AML HL60 cells were extensively studied. In total, 5,775 proteins and 1,124 lysine acetylation sites were successfully obtained in response to VAP and SAHA treatment. It is found that VPA and SAHA treatment differently induced proteome and acetylome profiling in AML HL60 cells. This study revealed the differential impacts of VPA and SAHA on proteome/acetylome in AML cells, deepening our understanding of HDAC inhibitor mediated AML therapeutics.

Differentiation-promoting activity of pomegranate (Punica granatum) fruit extracts in HL-60 human promyelocytic leukemia cells.

PubMed

Kawaii, Satoru; Lansky, Ephraim P

2004-01-01

Differentiation refers to the ability of cancer cells to revert to their normal counterparts, and its induction represents an important noncytotoxic therapy for leukemia, and also breast, prostate, and other solid malignancies. Flavonoids are a group of differentiation-inducing chemicals with a potentially lower toxicology profile than retinoids. Flavonoid-rich polyphenol fractions from the pomegranate (Punica granatum) fruit exert anti-proliferative, anti-invasive, anti-eicosanoid, and pro-apoptotic actions in breast and prostate cancer cells and anti-angiogenic activities in vitro and in vivo. Here we tested flavonoid-rich fractions from fresh (J) and fermented (W) pomegranate juice and from an aqueous extraction of pomegranate pericarps (P) as potential differentiation-promoting agents of human HL-60 promyelocytic leukemia cells. Four assays were used to assess differentiation: nitro blue tetrazolium reducing activity, nonspecific esterase activity, specific esterase activity, and phagocytic activity. In addition, the effect of these extracts on HL-60 proliferation was evaluated. Extracts W and P were strong promoters of differentiation in all settings, with extract J showing only a relatively mild differentiation-promoting effect. The extracts had proportional inhibitory effects on HL-60 cell proliferation. The results highlight an important, previously unknown, mechanism of the cancer preventive and suppressive potential of pomegranate fermented juice and pericarp extracts.

Anticancer activity and mediation of apoptosis in human HL-60 leukaemia cells by edible sea cucumber (Holothuria edulis) extract.

PubMed

Wijesinghe, W A J P; Jeon, You Jin; Ramasamy, Perumal; Wahid, Mohd Effendy A; Vairappan, Charles S

2013-08-15

Sea cucumbers have been a dietary delicacy and important ingredient in Asian traditional medicinal over many centuries. In this study, edible sea cucumber Holothuria edulis was evaluated for its in vitro anticancer potential. An aqueous fraction of the edible sea cucumber (ESC-AQ) has been shown to deliver a strong cytotoxic effect against the human HL-60 leukaemia cell line. An induction effect of apoptotic body formation in response to ESC-AQ treatment was confirmed in HL-60 cells stained with Hoechst 33342 and confirmed via flow cytometry analysis. The up regulation of Bax and caspase-3 protein expression was observed while the expression of Bcl-xL protein was down regulated in ESC-AQ treated HL-60 cells. Due to the profound anticancer activity, ESC-AQ appears to be an economically important biomass fraction that can be exploited in numerous industrial applications as a source of functional ingredients. Copyright © 2013 Elsevier Ltd. All rights reserved.

Numerical optimization of perturbative coils for tokamaks

NASA Astrophysics Data System (ADS)

Lazerson, Samuel; Park, Jong-Kyu; Logan, Nikolas; Boozer, Allen; NSTX-U Research Team

2014-10-01

Numerical optimization of coils which apply three dimensional (3D) perturbative fields to tokamaks is presented. The application of perturbative 3D magnetic fields in tokamaks is now commonplace for control of error fields, resistive wall modes, resonant field drive, and neoclassical toroidal viscosity (NTV) torques. The design of such systems has focused on control of toroidal mode number, with coil shapes based on simple window-pane designs. In this work, a numerical optimization suite based on the STELLOPT 3D equilibrium optimization code is presented. The new code, IPECOPT, replaces the VMEC equilibrium code with the IPEC perturbed equilibrium code, and targets NTV torque by coupling to the PENT code. Fixed boundary optimizations of the 3D fields for the NSTX-U experiment are underway. Initial results suggest NTV torques can be driven by normal field spectrums which are not pitch-resonant with the magnetic field lines. Work has focused on driving core torque with n = 1 and edge torques with n = 3 fields. Optimizations of the coil currents for the planned NSTX-U NCC coils highlight the code's free boundary capability. This manuscript has been authored by Princeton University under Contract Number DE-AC02-09CH11466 with the U.S. Department of Energy.

The conceptual design of a robust, compact, modular tokamak reactor based on high-field superconductors

NASA Astrophysics Data System (ADS)

Whyte, D. G.; Bonoli, P.; Barnard, H.; Haakonsen, C.; Hartwig, Z.; Kasten, C.; Palmer, T.; Sung, C.; Sutherland, D.; Bromberg, L.; Mangiarotti, F.; Goh, J.; Sorbom, B.; Sierchio, J.; Ball, J.; Greenwald, M.; Olynyk, G.; Minervini, J.

2012-10-01

Two of the greatest challenges to tokamak reactors are 1) large single-unit cost of each reactor's construction and 2) their susceptibility to disruptions from operation at or above operational limits. We present an attractive tokamak reactor design that substantially lessens these issues by exploiting recent advancements in superconductor (SC) tapes allowing peak field on SC coil > 20 Tesla. A R˜3.3 m, B˜9.2 T, ˜ 500 MW fusion power tokamak provides high fusion gain while avoiding all disruptive operating boundaries (no-wall beta, kink, and density limits). Robust steady-state core scenarios are obtained by exploiting the synergy of high field, compact size and ideal efficiency current drive using high-field side launch of Lower Hybrid waves. The design features a completely modular replacement of internal solid components enabled by the demountability of the coils/tapes and the use of an immersion liquid blanket. This modularity opens up the possibility of using the device as a nuclear component test facility.

Energetic particles in spherical tokamak plasmas

NASA Astrophysics Data System (ADS)

McClements, K. G.; Fredrickson, E. D.

2017-05-01

Spherical tokamaks (STs) typically have lower magnetic fields than conventional tokamaks, but similar mass densities. Suprathermal ions with relatively modest energies, in particular beam-injected ions, consequently have speeds close to or exceeding the Alfvén velocity, and can therefore excite a range of Alfvénic instabilities which could be driven by (and affect the behaviour of) fusion α-particles in a burning plasma. STs heated with neutral beams, including the small tight aspect ratio tokamak (START), the mega amp spherical tokamak (MAST), the national spherical torus experiment (NSTX) and Globus-M, have thus provided an opportunity to study toroidal Alfvén eigenmodes (TAEs), together with higher frequency global Alfvén eigenmodes (GAEs) and compressional Alfvén eigenmodes (CAEs), which could affect beam current drive and channel fast ion energy into bulk ions in future devices. In NSTX GAEs were correlated with a degradation of core electron energy confinement. In MAST pulses with reduced magnetic field, CAEs were excited across a wide range of frequencies, extending to the ion cyclotron range, but were suppressed when hydrogen was introduced to the deuterium plasma, apparently due to mode conversion at ion-ion hybrid resonances. At lower frequencies fishbone instabilities caused fast particle redistribution in some MAST and NSTX pulses, but this could be avoided by moving the neutral beam line away from the magnetic axis or by operating the plasma at either high density or elevated safety factor. Fast ion redistribution has been observed during GAE avalanches on NSTX, while in both NSTX and MAST fast ions were transported by saturated kink modes, sawtooth crashes, resonant magnetic perturbations and TAEs. The energy dependence of fast ion redistribution due to both sawteeth and TAEs has been studied in Globus-M. High energy charged fusion products are unconfined in present-day STs, but have been shown in MAST to provide a useful diagnostic of beam ion

Energetic particles in spherical tokamak plasmas

DOE PAGES

McClements, K. G.; Fredrickson, E. D.

2017-03-21

Spherical tokamaks (STs) typically have lower magnetic fields than conventional tokamaks, but similar mass densities. Suprathermal ions with relatively modest energies, in particular beam-injected ions, consequently have speeds close to or exceeding the Alfvén velocity, and can therefore excite a range of Alfvénic instabilities which could be driven by (and affect the behaviour of) fusion α-particles in a burning plasma. STs heated with neutral beams, including the small tight aspect ratio tokamak (START), the mega amp spherical tokamak (MAST), the national spherical torus experiment (NSTX) and Globus-M, have thus provided an opportunity to study toroidal Alfvén eigenmodes (TAEs), together withmore » higher frequency global Alfvén eigenmodes (GAEs) and compressional Alfvén eigenmodes (CAEs), which could affect beam current drive and channel fast ion energy into bulk ions in future devices. In NSTX GAEs were correlated with a degradation of core electron energy confinement. In MAST pulses with reduced magnetic field, CAEs were excited across a wide range of frequencies, extending to the ion cyclotron range, but were suppressed when hydrogen was introduced to the deuterium plasma, apparently due to mode conversion at ion–ion hybrid resonances. At lower frequencies fishbone instabilities caused fast particle redistribution in some MAST and NSTX pulses, but this could be avoided by moving the neutral beam line away from the magnetic axis or by operating the plasma at either high density or elevated safety factor. Fast ion redistribution has been observed during GAE avalanches on NSTX, while in both NSTX and MAST fast ions were transported by saturated kink modes, sawtooth crashes, resonant magnetic perturbations and TAEs. The energy dependence of fast ion redistribution due to both sawteeth and TAEs has been studied in Globus-M. High energy charged fusion products are unconfined in present-day STs, but have been shown in MAST to provide a useful diagnostic of

Design and fabrication of the NASA HL-20 support cradle and interior mockup

NASA Technical Reports Server (NTRS)

Exum, Thurman

1991-01-01

An extensive test program involving analysis in both the horizontal and vertical attitudes of the HL-20 will be conducted by NASA-Langley. This necessitated the fabrication of a steel support cradle for the composite Personnel Launch System (PLS) model and an internal mockup to simulate the pilot and passenger compartments.

First neutral beam injection experiments on KSTAR tokamak.

PubMed

Jeong, S H; Chang, D H; Kim, T S; In, S R; Lee, K W; Jin, J T; Chang, D S; Oh, B H; Bae, Y S; Kim, J S; Park, H T; Watanabe, K; Inoue, T; Kashiwagi, M; Dairaku, M; Tobari, H; Hanada, M

2012-02-01

The first neutral beam (NB) injection system of the Korea Superconducting Tokamak Advanced Research (KSTAR) tokamak was partially completed in 2010 with only 1∕3 of its full design capability, and NB heating experiments were carried out during the 2010 KSTAR operation campaign. The ion source is composed of a JAEA bucket plasma generator and a KAERI large multi-aperture accelerator assembly, which is designed to deliver a 1.5 MW, NB power of deuterium at 95 keV. Before the beam injection experiments, discharge, and beam extraction characteristics of the ion source were investigated. The ion source has good beam optics in a broad range of beam perveance. The optimum perveance is 1.1-1.3 μP, and the minimum beam divergence angle measured by the Doppler shift spectroscopy is 0.8°. The ion species ratio is D(+):D(2)(+):D(3)(+) = 75:20:5 at beam current density of 85 mA/cm(2). The arc efficiency is more than 1.0 A∕kW. In the 2010 KSTAR campaign, a deuterium NB power of 0.7-1.5 MW was successfully injected into the KSTAR plasma with a beam energy of 70-90 keV. L-H transitions were observed within a wide range of beam powers relative to a threshold value. The edge pedestal formation in the T(i) and T(e) profiles was verified through CES and electron cyclotron emission diagnostics. In every deuterium NB injection, a burst of D-D neutrons was recorded, and increases in the ion temperature and plasma stored energy were found.

S1PR1 drives a feedforward signalling loop to regulate BATF3 and the transcriptional programme of Hodgkin lymphoma cells

PubMed Central

Vrzalikova, K; Ibrahim, M; Vockerodt, M; Perry, T; Margielewska, S; Lupino, L; Nagy, E; Soilleux, E; Liebelt, D; Hollows, R; Last, A; Reynolds, G; Abdullah, M; Curley, H; Care, M; Krappmann, D; Tooze, R; Allegood, J; Spiegel, S; Wei, W; Woodman, C B J; Murray, P G

2018-01-01

The Hodgkin/Reed–Sternberg cells of classical Hodgkin lymphoma (HL) are characterised by the aberrant activation of multiple signalling pathways. Here we show that a subset of HL displays altered expression of sphingosine-1-phosphate (S1P) receptors (S1PR)s. S1P activates phosphatidylinositide 3-kinase (PI3-K) in these cells that is mediated by the increased expression of S1PR1 and the decreased expression of S1PR2. We also showed that genes regulated by the PI3-K signalling pathway in HL cell lines significantly overlap with the transcriptional programme of primary HRS cells. Genes upregulated by the PI3-K pathway included the basic leucine zipper transcription factor, ATF-like 3 (BATF3), which is normally associated with the development of dendritic cells. Immunohistochemistry confirmed that BATF3 was expressed in HRS cells of most HL cases. In contrast, in normal lymphoid tissues, BATF3 expression was confined to a small fraction of CD30-positive immunoblasts. Knockdown of BATF3 in HL cell lines revealed that BATF3 contributed to the transcriptional programme of primary HRS cells, including the upregulation of S1PR1. Our data suggest that disruption of this potentially oncogenic feedforward S1P signalling loop could provide novel therapeutic opportunities for patients with HL. PMID:28878352

Electron Cyclotron Current Drive Efficiency in General Tokamak Geometry and Its Application to Advanced Tokamak Plasmas

NASA Astrophysics Data System (ADS)

Lin-Liu, Y. R.; Chan, V. S.; Luce, T. C.; Prater, R.

1998-11-01

Owing to relativistic mass shift in the cyclotron resonance condition, a simple and accurate interpolation formula for estimating the current drive efficiency, such as those(S.C. Chiu et al.), Nucl. Fusion 29, 2175 (1989).^,(D.A. Ehst and C.F.F. Karney, Nucl. Fusion 31), 1933 (1991). commonly used in FWCD, is not available in the case of ECCD. In this work, we model ECCD using the adjoint techniques. A semi-analytic adjoint function appropriate for general tokamak geometry is obtained using Fisch's relativistic collision model. Predictions of off-axis ECCD qualitatively and semi-quantitatively agrees with those of Cohen,(R.H. Cohen, Phys. Fluids 30), 2442 (1987). currently implemented in the raytracing code TORAY. The dependences of the current drive efficiency on the wave launch configuration and the plasma parameters will be presented. Strong absorption of the wave away from the resonance layer is shown to be an important factor in optimizing the off-axis ECCD for application to advanced tokamak operations.

A self-consistent model of an isothermal tokamak

NASA Astrophysics Data System (ADS)

McNamara, Steven; Lilley, Matthew

2014-10-01

Continued progress in liquid lithium coating technologies have made the development of a beam driven tokamak with minimal edge recycling a feasibly possibility. Such devices are characterised by improved confinement due to their inherent stability and the suppression of thermal conduction. Particle and energy confinement become intrinsically linked and the plasma thermal energy content is governed by the injected beam. A self-consistent model of a purely beam fuelled isothermal tokamak is presented, including calculations of the density profile, bulk species temperature ratios and the fusion output. Stability considerations constrain the operating parameters and regions of stable operation are identified and their suitability to potential reactor applications discussed.

Topic maps for exploring nosological, lexical, semantic and HL7 structures for clinical data.

PubMed

Paterson, Grace I; Grant, Andrew M; Soroka, Steven D

2008-12-01

A topic map is implemented for learning about clinical data associated with a hospital stay for patients diagnosed with chronic kidney disease, diabetes and hypertension. The question posed is: how might a topic map help bridge perspectival differences among communities of practice and help make commensurable the different classifications they use? The knowledge layer of the topic map was generated from existing ontological relationships in nosological, lexical, semantic and HL7 boundary objects. Discharge summaries, patient charts and clinical data warehouse entries rectified the clinical knowledge used in practice. These clinical data were normalized to HL7 Clinical Document Architecture (CDA) markup standard and stored in the Clinical Document Repository. Each CDA entry was given a subject identifier and linked with the topic map. The ability of topic maps to function as the infostructure ;glue' is assessed using dimensions of semantic interoperability and commensurability.

Currents in the DIII-D Tokamak

NASA Astrophysics Data System (ADS)

Azari, A.; Eidietis, N. W.

2012-10-01

Loss of vertical control of an elongated tokamak plasma results in a vertical displacement event (VDE) which can induce large currents on open field lines and exert high JxB forces on in-vessel components. An array of first-wall tile current monitors on DIII-D provides direct measurement of the poloidal halo currents. These measurements are analyzed to create a database of halo current magnitude and asymmetry, which are found to lie within the ranges seen by numerous other tokamaks in the ITPA Disruption Database. In addition, an analysis of halo asymmetry rotation is presented, as rotation at the resonance frequencies of in-vessel components could lead to significant amplification of the halo forces. Halo current rotation is found to be far more prevalent in old (1997-2002) DIII-D halo current data than recent data (2009), perhaps due to a change in divertor geometry over that time.

The Physics of Tokamak Start-up

DOE Office of Scientific and Technical Information (OSTI.GOV)

D. Mueller

Tokamak start-up on present-day devices usually relies on inductively induced voltage from a central solenoid. In some cases inductive startup is assisted with auxiliary power from electron cyclotron radio frequency heating. ITER, the National Spherical Torus eXperiment Upgrade and JT60, now under construction, will make use of the understanding gained from present-day devices to ensure successful start-up. Design of a spherical tokamak (ST) with DT capability for nuclear component testing would require an alternative to a central solenoid because the small central column in an ST has insufficient space to provide shielding for the insulators in the solenoid. Alternative start-upmore » techniques such as induction using outer poloidal field coils, electron Bernstein wave start-up, coaxial helicity injection and point source helicity injection have been used with success, but require demonstration of scaling to higher plasma current.« less

The physics of tokamak start-up

DOE Office of Scientific and Technical Information (OSTI.GOV)

Mueller, D.

Tokamak start-up on present-day devices usually relies on inductively induced voltage from a central solenoid. In some cases, inductive startup is assisted with auxiliary power from electron cyclotron radio frequency heating. International Thermonuclear Experimental Reactor, the National Spherical Torus Experiment Upgrade and JT60, now under construction, will make use of the understanding gained from present-day devices to ensure successful start-up. Design of a spherical tokamak (ST) with DT capability for nuclear component testing would require an alternative to a central solenoid because the small central column in an ST has insufficient space to provide shielding for the insulators in themore » solenoid. Alternative start-up techniques such as induction using outer poloidal field coils, electron Bernstein wave start-up, coaxial helicity injection, and point source helicity injection have been used with success, but require demonstration of scaling to higher plasma current.« less

Merging-compression formation of high temperature tokamak plasma

NASA Astrophysics Data System (ADS)

Gryaznevich, M. P.; Sykes, A.

2017-07-01

Merging-compression is a solenoid-free plasma formation method used in spherical tokamaks (STs). Two plasma rings are formed and merged via magnetic reconnection into one plasma ring that then is radially compressed to form the ST configuration. Plasma currents of several hundred kA and plasma temperatures in the keV-range have been produced using this method, however until recently there was no full understanding of the merging-compression formation physics. In this paper we explain in detail, for the first time, all stages of the merging-compression plasma formation. This method will be used to create ST plasmas in the compact (R ~ 0.4-0.6 m) high field, high current (3 T/2 MA) ST40 tokamak. Moderate extrapolation from the available experimental data suggests the possibility of achieving plasma current ~2 MA, and 10 keV range temperatures at densities ~1-5  ×  1020 m-3, bringing ST40 plasmas into a burning plasma (alpha particle heating) relevant conditions directly from the plasma formation. Issues connected with this approach for ST40 and future ST reactors are discussed

Saturated Widths of Magnetic Islands in Tokamak Discharges

NASA Astrophysics Data System (ADS)

Halpern, F.; Pankin, A. Y.

2005-10-01

The new ISLAND module described in reference [1] implements a quasi-linear model to compute the widths of multiple magnetic islands driven by saturated tearing modes in toroidal plasmas of arbitrary aspect ratio and cross sectional shape. The distortion of the island shape caused by the radial variation in the perturbation is computed in the new module. In transport simulations, the enhanced transport caused by the magnetic islands has the effect of flattening the pressure and current density profiles. This self consistent treatment of the magnetic islands alters the development of the plasma profiles. In addition, it is found that islands closer to the magnetic axis influence the evolution of islands further out in the plasma. In order to investigate such phenomena, the ISLAND module is used within the BALDUR predictive modeling code to compute the widths of multiple magnetic islands in tokamak discharges. The interaction between the islands and sawtooth crashes is examined in simulations of DIII-D and JET discharges. The module is used to compute saturated neoclassical tearing mode island widths for multiple modes in ITER. Preliminary results for island widths in ITER are consistent with those presented [2] by Hegna. [1] F.D. Halpern, G. Bateman, A.H. Kritz and A.Y. Pankin, ``The ISLAND Module for Computing Magnetic Island Widths in Tokamaks,'' submitted to J. Plasma Physics (2005). [2] C.C. Hegna, 2002 Fusion Snowmass Meeting.

Full orbit computations of ripple-induced fusion {alpha}-particle losses from burning tokamak plasmas

DOE Office of Scientific and Technical Information (OSTI.GOV)

McClements, K.G.

A full orbit code is used to compute collisionless losses of fusion {alpha} particles from three proposed burning plasma tokamaks: the International Tokamak Experimental Reactor (ITER); a spherical tokamak power plant (STPP) [T. C. Hender, A. Bond, J. Edwards, P. J. Karditsas, K. G. McClements, J. Mustoe, D. V. Sherwood, G. M. Voss, and H. R. Wilson, Fusion Eng. Des. 48, 255 (2000)]; and a spherical tokamak components test facility (CTF) [H. R. Wilson, G. M. Voss, R. J. Akers, L. Appel, A. Dnestrovskij, O. Keating, T. C. Hender, M. J. Hole, G. Huysmans, A. Kirk, P. J. Knight, M.more » Loughlin, K. G. McClements, M. R. O'Brien, and D. Yu. Sychugov, Proceedings of the 20th IAEA Fusion Energy Conference, Invited Paper FT/3-1Ra]. It has been suggested that {alpha} particle transport could be enhanced due to cyclotron resonance with the toroidal magnetic field ripple. However, calculations for inductive operation in ITER yield a loss rate that appears to be broadly consistent with the predictions of guiding center theory, falling monotonically as the number of toroidal field coils N is increased (and hence the ripple amplitude is decreased). For STPP and CTF the loss rate does not decrease monotonically with N, but collisionless losses are generally low in absolute terms. As in the case of ITER, there is no evidence that finite Larmor radius effects would seriously degrade fusion {alpha}-particle confinement.« less

The synergistic antitumor activity of arsenic trioxide and vitamin K2 in HL-60 cells involves increased ROS generation and regulation of the ROS-dependent MAPK signaling pathway.

PubMed

Qu, Hui; Tong, Danan; Zhang, Yanqing; Kang, Kai; Zhang, Yuling; Chen, Lan; Ren, Lihong

2013-10-01

The aim of this study was to investigate the synergistic anticancer effects of arsenic trioxide (ATO) and vitamin K2 (VK2) in HL-60 cells, and elucidate the potential mechanisms. HL-60 cells were exposed to ATO and VK2, either alone or in combination. Cell proliferation and apoptosis were assessed. The combination index (CI) method was used to evaluate whether the action of the drug combination was synergistic, additive or antagonistic. Reactive oxygen species (ROS) and the mitogen-activated protein kinase (MAPK) signaling pathway were also studied, to provide insight into potential mechanisms. The results showed that combining ATO with VK2 significantly inhibited HL-60 cell growth more than either agent alone, indicating a synergistic effect with CI < 1. Annexin V staining demonstrated that the inhibition of cell growth by the drug combination was mediated through an increase in apoptosis; this was supported by examination of caspase-3 and caspase-9 with Western blot assays. Furthermore, induction of ROS, and phosphorylation and activation of the JNK and p38 (but not ERK1/2) pathways, was observed in cells administered the drug combination. Prior treatment with the antioxidant, N-acetylcysteine, partly blocked the apoptosis and expression of caspase-3 induced by the drug combination; apoptosis and expression of caspase-3 were also reversed by inhibitors of JNK or p38. These results suggest that ATO and VK2 act synergistically to increase HL-60 cell apoptosis, through ROS generation and regulation of the MAPK signaling pathway.

Simulations of Tokamak Edge Turbulence Including Self-Consistent Zonal Flows

NASA Astrophysics Data System (ADS)

Cohen, Bruce; Umansky, Maxim

2013-10-01

Progress on simulations of electromagnetic drift-resistive ballooning turbulence in the tokamak edge is summarized in this mini-conference talk. A more detailed report on this work is presented in a poster at this conference. This work extends our previous work to include self-consistent zonal flows and their effects. The previous work addressed the simulation of L-mode tokamak edge turbulence using the turbulence code BOUT. The calculations used realistic single-null geometry and plasma parameters of the DIII-D tokamak and produced fluctuation amplitudes, fluctuation spectra, and particle and thermal fluxes that compare favorably to experimental data. In the effect of sheared ExB poloidal rotation is included with an imposed static radial electric field fitted to experimental data. In the new work here we include the radial electric field self-consistently driven by the microturbulence, which contributes to the sheared ExB poloidal rotation (zonal flow generation). We present simulations with/without zonal flows for both cylindrical geometry, as in the UCLA Large Plasma Device, and for the DIII-D tokamak L-mode cases in to quantify the influence of self-consistent zonal flows on the microturbulence and the concomitant transport. This work was performed under the auspices of the US Department of Energy under contract DE-AC52-07NA27344 at the Lawrence Livermore National Laboratory.

Three-dimensional analysis of tokamaks and stellarators

PubMed Central

Garabedian, Paul R.

2008-01-01

The NSTAB equilibrium and stability code and the TRAN Monte Carlo transport code furnish a simple but effective numerical simulation of essential features of present tokamak and stellarator experiments. When the mesh size is comparable to the island width, an accurate radial difference scheme in conservation form captures magnetic islands successfully despite a nested surface hypothesis imposed by the mathematics. Three-dimensional asymmetries in bifurcated numerical solutions of the axially symmetric tokamak problem are relevant to the observation of unstable neoclassical tearing modes and edge localized modes in experiments. Islands in compact stellarators with quasiaxial symmetry are easier to control, so these configurations will become good candidates for magnetic fusion if difficulties with safety and stability are encountered in the International Thermonuclear Experimental Reactor (ITER) project. PMID:18768807

Apoptosis and reduced cell proliferation of HL-60 cell line caused by human telomerase reverse transcriptase inhibition by siRNA.

PubMed

Miri-Moghaddam, Ebrahim; Deezagi, Abdolkhaleg; Soheili, Zahra Sohaila; Shariati, Parvin

2010-01-01

The close correlation between telomerase activity and human telomerase reverse transcriptase (hTERT) expression has made hTERT to be considered as a selective molecular target for human cancer therapy. In this study, the ability of short-interfering RNA (siRNA) to downregulate hTERT expression and its correlation with cell growth and apoptosis in the promyelocytic cell line HL-60 was evaluated. hTERT siRNA was designed and transfected to HL-60. hTERT mRNA expression, cell proliferation and apoptotic cells were measured. The results indicated that hTERT siRNA resulted in 97.2 ± 0.6% downregulation of the hTERT mRNA content; inhibition of the cell proliferation rate was about 52.8 ± 2.3% and the apoptotic index of cells was 30.5 ± 1.5%. hTERT plays an essential role in cell proliferation and control of the viability of leukemic cells, thus promising the development of drugs for leukemia. Copyright © 2010 S. Karger AG, Basel.

NADPH oxidase-mediated generation of reactive oxygen species is critically required for survival of undifferentiated human promyelocytic leukemia cell line HL-60.

PubMed

Dong, Jing-Mei; Zhao, Sheng-Guo; Huang, Guo-Yin; Liu, Qing

2004-06-01

Nicotinamide adenine dinucleotide phosphate oxidase (NADPH oxidase) mediated generation of reactive oxygen species (ROS) was originally identified as the powerful host defense machinery against microorganism in phagocytes. But recent reports indicated that some non-phagocytic cells also have the NADPH oxidase activity, and the ROS produced by it may act as cell signal molecule. But as far as today, whether the NADPH oxidase also plays similar role in phagocyte has not been paid much attention. Utilizing the undifferentiated HL-60 promyelocytic leukemia cells as a model, the aim of the present study was to determine whether NADPH oxidase plays a role on ROS generation in undifferentiated HL-60, and the ROS mediated by it was essential for cell's survival. For the first time, we verified that the release of ROS in undifferentiated HL-60 was significantly increased by the stimulation with Calcium ionophore or opsonized zymosan, which are known to trigger respiration burst in phagocytes by NADPH oxidase pathway. Diphenylene iodonium (DPI) or apocynin (APO), two inhibitors of NADPH oxidase, significantly suppressed the increasing of ROS caused by opsonized zymosan. Cell survival assay and fluorescence double dyeing with acridine orange and ethidium bromide showed that DPI and APO, as well as superoxide dismutase (SOD) and catalase (CAT) concentration-dependently decreased the viability of undifferentiated HL-60 cells, whereas exogenous H2O2 can rescue the cells from death obviously. Our results suggested that the ROS, generated by NADPH oxidase play an essential role in the survival of undifferentiated HL-60 cells.

Nonlinear Fluid Model Of 3-D Field Effects In Tokamak Plasmas

NASA Astrophysics Data System (ADS)

Callen, J. D.; Hegna, C. C.; Beidler, M. T.

2017-10-01

Extended MHD codes (e.g., NIMROD, M3D-C1) are beginning to explore nonlinear effects of small 3-D magnetic fields on tokamak plasmas. To facilitate development of analogous physically understandable reduced models, a fluid-based dynamic nonlinear model of these added 3-D field effects in the base axisymmetric tokamak magnetic field geometry is being developed. The model incorporates kinetic-based closures within an extended MHD framework. Key 3-D field effects models that have been developed include: 1) a comprehensive modified Rutherford equation for the growth of a magnetic island that includes the classical tearing and NTM perturbed bootstrap current drives, externally applied magnetic field and current drives, and classical and neoclassical polarization current effects, and 2) dynamic nonlinear evolution of the plasma toroidal flow (radial electric field) in response to the 3-D fields. An application of this model to RMP ELM suppression precipitated by an ELM crash will be discussed. Supported by Office of Fusion Energy Sciences, Office of Science, Dept. of Energy Grants DE-FG02-86ER53218 and DE-FG02-92ER54139.

Simulations of Turbulence in Tokamak Edge and Effects of Self-Consistent Zonal Flows

NASA Astrophysics Data System (ADS)

Cohen, Bruce; Umansky, Maxim

2013-10-01

Progress is reported on simulations of electromagnetic drift-resistive ballooning turbulence in the tokamak edge. This extends previous work to include self-consistent zonal flows and their effects. The previous work addressed simulation of L-mode tokamak edge turbulence using the turbulence code BOUT that solves Braginskii-based plasma fluid equations in tokamak edge domain. The calculations use realistic single-null geometry and plasma parameters of the DIII-D tokamak and produce fluctuation amplitudes, fluctuation spectra, and particle and thermal fluxes that compare favorably to experimental data. In the effect of sheared ExB poloidal rotation is included with an imposed static radial electric field fitted to experimental data. In the new work here we include the radial electric field self-consistently driven by the microturbulence, which contributes to the sheared ExB poloidal rotation (zonal flow generation). We present simulations with/without zonal flows for both cylindrical geometry, as in the UCLA Large Plasma Device, and for the DIII-D tokamak L-mode cases in to quantify the influence of self-consistent zonal flows on the microturbulence and the concomitant transport. This work was performed under the auspices of the U.S. Department of Energy under contract DE-AC52-07NA27344 at the Lawrence Livermore National Laboratory.

Multi-field plasma sandpile model in tokamaks and applications

NASA Astrophysics Data System (ADS)

Peng, X. D.; Xu, J. Q.

2016-08-01

A multi-field sandpile model of tokamak plasmas is formulated for the first time to simulate the dynamic process with interaction between avalanche events on the fast/micro time-scale and diffusive transports on the slow/macro time-scale. The main characteristics of the model are that both particle and energy avalanches of sand grains are taken into account simultaneously. New redistribution rules of a sand-relaxing process are defined according to the transport properties of special turbulence which allows the uphill particle transport. Applying the model, we first simulate the steady-state plasma profile self-sustained by drift wave turbulences in the Ohmic discharge of a tokamak. A scaling law as f = a q0 b + c for the relation of both center-density n ( 0 ) and electron (ion) temperatures T e ( 0 ) ( T i ( 0 ) ) with the center-safety-factor q 0 is found. Then interesting work about the nonlocal transport phenomenon observed in tokamak experiments proceeds. It is found that the core electron temperature increases rapidly in response to the edge cold pulse and inversely it decreases in response to the edge heat pulse. The results show that the nonlocal response of core electron temperature depending on the amplitudes of background plasma density and temperature is more remarkable in a range of gas injection rate. Analyses indicate that the avalanche transport caused by plasma drift instabilities with thresholds is a possible physical mechanism for the nonlocal transport in tokamaks. It is believed that the model is capable of being applied to more extensive questions occurring in the transport field.

Experimental observation of m/n = 1/1 mode behaviour during sawtooth activity and its manifestations in tokamak plasmas

NASA Astrophysics Data System (ADS)

Udintsev, V. S.; Ottaviani, M.; Maget, P.; Giruzzi, G.; Ségui, J.-L.; Aniel, T.; Artaud, J. F.; Clairet, F.; Goniche, M.; Hoang, G. T.; Huysmans, G. T. A.; Imbeaux, F.; Joffrin, E.; Mazon, D.; Pecquet, A. L.; Sabot, R.; Sirinelli, A.; Vermare, L.; Tore Supra Team; Krämer-Flecken, A.; Koslowski, H. R.; TEXTOR Team; Donné, A. J. H.; Schüller, F. C.; Domier, C. W.; Luhmann, N. C., Jr.; Mirnov, S. V.

2005-08-01

To shed some light on the development of the fast m/n = 1/1 precursor to the sawtooth crash and its influence on plasma transport properties in the vicinity of the q = 1 surface, series of dedicated experiments have been conducted on the Tore Supra and TEXTOR tokamaks. It has been concluded that, before a crash, the hot core gets displaced with respect to the magnetic axis, drifts outwards by as much as 8-10 cm and may change its shape. Observation of the magnetic reconnection process has been made by means of electron cyclotron emission diagnostics. The heat pulse is seen far outside the inversion radius. The colder plasma develops a magnetic island on the former magnetic axis, after the hot core expulsion. Different kinds of behaviour of the m = 1 precursor before the crash, with respect to the displacement of the hot core and the duration of the oscillating phase, have been observed. An ideal kink model alone cannot be used for explanation; therefore, resistive effects play an important role in the mode development. Possible mechanisms that lead an m = 1 mode to such behaviour, and their links to the change in the central q-profile, are discussed. Results have been discussed in the light of various theoretical models of the sawtooth. This is an extended version of the paper presented at the 31st EPS Conf. on Plasma Physics (London, 28 June-2 July 2004).

Dibenzoyl and isoflavonoid glycosides from Sophora flavescens: inhibition of the cytotoxic effect of D-galactosamine on human hepatocyte HL-7702.

PubMed

Shen, Yi; Feng, Zi-Ming; Jiang, Jian-Shuang; Yang, Ya-Nan; Zhang, Pei-Cheng

2013-12-27

Twelve new dibenzoyl derivatives sophodibenzoside A-L (1-12) and five new isoflavone glycosides (13-17) have been isolated from the roots of Sophora flavescens together with eight known compounds (18-25). Notably, the use of acetic acid-d4 was required to enable identification of the dibenzoyl glycoside structures. Compounds 1, 2, 13, 14, and 19 exhibited weak inhibition of the cytotoxic effect of d-galactosamine on the human hepatic cell line HL-7702.

Antileukemic Potential of Momordica charantia Seed Extracts on Human Myeloid Leukemic HL60 Cells

PubMed Central

Soundararajan, Ramani; Prabha, Punit; Rai, Umesh; Dixit, Aparna

2012-01-01

Momordica charantia (bitter gourd) has been used in the traditional system of medicine for the treatment of various diseases. Anticancer activity of M. charantia extracts has been demonstrated by numerous in vitro and in vivo studies. In the present study, we investigated the differentiation inducing potential of fractionated M. charantia seed extracts in human myeloid HL60 cells. We found that the HL60 cells treated with the fractionated seed extracts differentiated into granulocytic lineage as characterized by NBT staining, CD11b expression, and specific esterase activity. The differentiation inducing principle was found to be heat-stable, and organic in nature. The differentiation was accompanied by a downregulation of c-myc transcript, indicating the involvement of c-myc pathway, at least in part, in differentiation. Taken together these results indicate that fractionated extracts of M. charantia seeds possess differentiation inducing activity and therefore can be evaluated for their potential use in differentiation therapy for leukemia in combination with other inducers of differentiation. PMID:22654956

Baking and helium glow discharge cleaning of SST-1 Tokamak with graphite plasma facing components

NASA Astrophysics Data System (ADS)

Semwal, P.; Khan, Z.; Raval, D. C.; Dhanani, K. R.; George, S.; Paravastu, Y.; Prakash, A.; Thankey, P.; Ramesh, G.; Khan, M. S.; Saikia, P.; Pradhan, S.

2017-04-01

Graphite plasma facing components (PFCs) were installed inside the SST-1 vacuum vessel. Prior to installation, all the graphite tiles were baked at 1000 °C in a vacuum furnace operated below 1.0 × 10-5 mbar. However due to the porous structure of graphite, they absorb a significant amount of water vapour from air during the installation process. Rapid desorption of this water vapour requires high temperature bake-out of the PFCs at ≥ 250 °C. In SST-1 the PFCs were baked at 250 °C using hot nitrogen gas facility to remove the absorbed water vapour. Also device with large graphite surface area has the disadvantage that a large quantity of hydrogen gets trapped inside it during plasma discharges which makes density control difficult. Helium glow discharge cleaning (He-GDC) effectively removes this stored hydrogen as well as other impurities like oxygen and hydrocarbon within few nano-meters from the surface by particle induced desorption. Before plasma operation in SST-1 tokamak, both baking of PFCs and He-GDC were carried out so that these impurities were removed effectively. The mean desorption yield of hydrogen was found to be 0.24. In this paper the results of baking and He-GDC experiments of SST-1 will be presented in detail.

Features of self-organized plasma physics in tokamaks

NASA Astrophysics Data System (ADS)

Razumova, K. A.

2018-01-01

The history of investigations the role of self-organization processes in tokamak plasma confinement is presented. It was experimentally shown that the normalized pressure profile is the same for different tokamaks. Instead of the conventional Fick equation, where the thermal flux is proportional to a pressure gradient, processes in the plasma are well described by the Dyabilanin’s energy balance equation, in which the heat flux is proportional to the difference of normalized gradients for self-consistent and real pressure profiles. The transport coefficient depends on the values of heat flux, which compensates distortion of the pressure profile with external impacts. Radiative cooling of the plasma edge decreases the heat flux and improves the confinement.

Workshop on High Power ICH Antenna Designs for High Density Tokamaks

NASA Astrophysics Data System (ADS)

Aamodt, R. E.

1990-02-01

A workshop in high power ICH antenna designs for high density tokamaks was held to: (1) review the data base relevant to the high power heating of high density tokamaks; (2) identify the important issues which need to be addressed in order to ensure the success of the ICRF programs on CIT and Alcator C-MOD; and (3) recommend approaches for resolving the issues in a timely realistic manner. Some specific performance goals for the antenna system define a successful design effort. Simply stated these goals are: couple the specified power per antenna into the desired ion species; produce no more than an acceptable level of RF auxiliary power induced impurities; and have a mechanical structure which safely survives the thermal, mechanical and radiation stresses in the relevant environment. These goals are intimately coupled and difficult tradeoffs between scientific and engineering constraints have to be made.

Adaptation to acidic soil is achieved by increased numbers of cis-acting elements regulating ALMT1 expression in Holcus lanatus.

PubMed

Chen, Zhi Chang; Yokosho, Kengo; Kashino, Miho; Zhao, Fang-Jie; Yamaji, Naoki; Ma, Jian Feng

2013-10-01

Yorkshire fog (Holcus lanatus), which belongs to the Poaceae family and is a close relative of the agronomic crop oat (Avena sativa), is a widely adaptable grass species that is able to grow on highly acidic soils with high levels of Al, but the mechanism underlying the high Al tolerance is unknown. Here, we characterized two accessions of H. lanatus collected from an acid plot (soil pH 3.6, HL-A) and a neutral plot (pH 7.1, HL-N) in terms of Al tolerance, organic acid anion secretion and related gene expression. In response to Al (pH 4.5), the HL-A roots secreted approximately twice as much malate as the HL-N roots, but there was no difference in citrate secretion. Cloning of the gene HlALMT1 responsible for malate secretion showed that the encoded amino acid sequence did not differ between two accessions, but the expression level in the outer cell layers of the HL-A roots was twice as high as in the HL-N roots. This difference was not due to the genomic copy number, but was due to the number of cis-acting elements for an Al-responsive transcription factor (HlART1) in the promoter region of HlALMT1, as demonstrated by both a yeast one-hybrid assay and a transient assay in tobacco protoplasts. Furthermore, introduction of HlALMT1 driven by the HL-A promoter into rice resulted in significantly more Al-induced malate secretion than introduction of HlALMT1 driven by the HL-N promoter. These findings indicate that the adaptation of H. lanatus to acidic soils may be achieved by increasing number of cis-acting elements for ART1 in the promoter region of the HlALMT1 gene, enhancing the expression of HlALMT1 and the secretion of malate. © 2013 The Authors The Plant Journal © 2013 John Wiley & Sons Ltd.

Antisense sequences of the nbl gene induce apoptosis in the human promyelocytic leukemia cell line HL-60.

PubMed

Naora, H; Nishida, T; Shindo, Y; Adachi, M; Naora, H

1998-04-01

Apoptosis is induced by the transcriptional inhibitor actinomycin D (Act D) in various cell types, particularly many leukemic cell lines such as HL-60. A common feature of these cell lines is their high constitutive expression level of the nbl gene, which was originally isolated by virtue of its abundance in a Namalwa Burkitt lymphoma cDNA library. In contrast, cell lines which constitutively express nbl at low levels appear not to undergo typical apoptotic death in response to Act D. Apoptotic induction by Act D in cells which normally express nbl at high levels was found in this study to be closely associated with a decline in nbl mRNA levels, raising the possibility that apoptosis could be induced by lowering nbl expression levels in such cells. Transient expression of nbl antisense sequences in HL-60 cells decreased cell viability, and induced typical apoptotic morphology such as cell shrinkage, chromatin condensation and nuclear fragmentation. Incubation with nbl antisense oligomers also induced similar features in HL-60 cells and in another high nb-expressing cell line, Jurkat, but had little effect in HepG2 cells which constitutively express nbl at low levels. These findings suggest that lowering constitutively high levels of nbl expression can induce apoptosis.

Expedition_55_Education_Event_HL_Suverkrup_Elementary_2018_061_1530_624314

NASA Image and Video Library

2018-03-02

SPACE STATION CREW MEMBERS DISCUSS LIFE IN SPACE WITH ARIZONA STUDENTS------ Aboard the International Space Station, Expedition 55 Flight Engineers Scott Tingle of NASA and Norishige Kanai of the Japan Aerospace Exploration Agency (JAXA) discussed life and research aboard the orbital outpost during an in-flight educational event March 2 with students at the HL Suverkrup Elementary School in Yuma, Arizona. Tingle and Kanai are in the midst of a five-and-a-half-month mission on the station.

MHD Effects of a Ferritic Wall on Tokamak Plasmas

NASA Astrophysics Data System (ADS)

Hughes, Paul E.

It has been recognized for some time that the very high fluence of fast (14.1MeV) neutrons produced by deuterium-tritium fusion will represent a major materials challenge for the development of next-generation fusion energy projects such as a fusion component test facility and demonstration fusion power reactor. The best-understood and most promising solutions presently available are a family of low-activation steels originally developed for use in fission reactors, but the ferromagnetic properties of these steels represent a danger to plasma confinement through enhancement of magnetohydrodynamic instabilities and increased susceptibility to error fields. At present, experimental research into the effects of ferromagnetic materials on MHD stability in toroidal geometry has been confined to demonstrating that it is still possible to operate an advanced tokamak in the presence of ferromagnetic components. In order to better quantify the effects of ferromagnetic materials on tokamak plasma stability, a new ferritic wall has been installated in the High Beta Tokamak---Extended Pulse (HBT-EP) device. The development, assembly, installation, and testing of this wall as a modular upgrade is described, and the effect of the wall on machine performance is characterized. Comparative studies of plasma dynamics with the ferritic wall close-fitting against similar plasmas with the ferritic wall retracted demonstrate substantial effects on plasma stability. Resonant magnetic perturbations (RMPs) are applied, demonstrating a 50% increase in n = 1 plasma response amplitude when the ferritic wall is near the plasma. Susceptibility of plasmas to disruption events increases by a factor of 2 or more with the ferritic wall inserted, as disruptions are observed earlier with greater frequency. Growth rates of external kink instabilities are observed to be twice as large in the presence of a close-fitting ferritic wall. Initial studies are made of the influence of mode rotation frequency

High-beta spherical tokamak startup in TS-4 merging experiment by use of toroidal field ramp-up

NASA Astrophysics Data System (ADS)

Kaminou, Yasuhiro; , Toru, II; Kato, Joji; Inomoto, Michiaki; Ono, Yasushi; TS Group Team; National InstituteFusion Science Collaboration

2014-10-01

We demonstrated the formation method of an ultrahigh-beta spherical tokamak by use of a field-reversed configuration and a spheromak in TS-4 device (R ~ 0.5 m, A ~ 1.5, Ip ~ 30-100 kA, B ~ 100 mT). This method is composed of the following steps: 1. Two spheromaks are merged together and a high-beta spheromak or FRC is formed by reconnection heating. 2. External toroidal magnetic field is added (current rising time ~50 μs), and spherical tokamak-like configuration is formed. In this way, the ultrahigh-beta ST is formed. The ultrahigh-beta ST formed by FRC has a diamagnetic toroidal field, and it presumed to be in a second-stable state for ballooning stability, and the one formed by spheromak has a weak paramagnetic toroidal magnetic field, while a spheormak has a strong paramagnetic toroidal magnetic field. This diamagnetic current derives from inductive electric field by ramping up the external toroidal magnetic field, and the diamagnetic current sustains high thermal pressure of the ultrahigh-beta spherical tokamak. And the beta of the ultrahigh-beta ST formed by FRC reaches about 50%. To sustain the high-beta state, 0.6 MW neutral beam injection and center solenoid coils are installed to the TS-4 device. In the poster, we report the experimental results of ultrahigh-beta spherical tokamak startup and sustainment by NBI and CS current driving experiment.

The Experiment of Modulated Toroidal Current on HT-7 and HT-6M Tokamak

NASA Astrophysics Data System (ADS)

Mao, Jian-shan; P, Phillips; Luo, Jia-rong; Xu, Yu-hong; Zhao, Jun-yu; Zhang, Xian-mei; Wan, Bao-nian; Zhang, Shou-yin; Jie, Yin-xian; Wu, Zhen-wei; Hu, Li-qun; Liu, Sheng-xia; Shi, Yue-jiang; Li, Jian-gang; HT-6M; HT-7 Group

2003-02-01

The Experiments of Modulated Toroidal Current were done on the HT-6M tokamak and HT-7 superconducting tokamak. The toroidal current was modulated by programming the Ohmic heating field. Modulation of the plasma current has been used successfully to suppress MHD activity in discharges near the density limit where large MHD m = 2 tearing modes were suppressed by sufficiently large plasma current oscillations. The improved Ohmic confinement phase was observed during modulating toroidal current (MTC) on the Hefei Tokamak-6M (HT-6M) and Hefei superconducting Tokamak-7 (HT-7). A toroidal frequency-modulated current, induced by a modulated loop voltage, was added on the plasma equilibrium current. The ratio of A.C. amplitude of plasma current to the main plasma current ΔIp/Ip is about 12%-30%. The different formats of the frequency-modulated toroidal current were compared.

Helical flow in RFX-mod tokamak plasmas

NASA Astrophysics Data System (ADS)

Piron, L.; Zaniol, B.; Bonfiglio, D.; Carraro, L.; Kirk, A.; Marrelli, L.; Martin, R.; Piron, C.; Piovesan, P.; Zuin, M.

2017-05-01

This work presents the first evidence of helical flow in RFX-mod q(a)  <  2 tokamak plasmas. The flow pattern is characterized by the presence of convective cells with m  =  1 and n  =  1 periodicity in the poloidal and toroidal directions, respectively. A similar helical flow deformation has been observed in the same device when operated as a reversed field pinch (RFP). In RFP plasmas, the flow dynamic is tailored by the innermost resonant m  =  1, n  =  7 tearing mode, which sustains the magnetic field configuration through the dynamo mechanism (Bonomo et al 2011 Nucl. Fusion 51 123007). By contrast, in the tokamak experiments presented here, it is strongly correlated with the m  =  1, n  =  1 MHD activity. A helical deformation of the flow pattern, associated with the deformation of the magnetic flux surfaces, is predicted by several codes, such as Specyl (Bonfiglio et al 2005 Phys. Rev. Lett. 94 145001), PIXIE3D (Chacón et al 2008 Phys. Plasmas 15 056103), NIMROD (King et al 2012 Phys. Plasmas 19 055905) and M3D-C1 (Jardin et al 2015 Phys. Rev. Lett. 115 215001). Among them, the 3D fully non-linear PIXIE3D has been used to calculate synthetic flow measurements, using a 2D flow modelling code. Inputs to the code are the PIXIE3D flow maps, the ion emission profiles as calculated by a 1D collisional radiative impurity transport code (Carraro et al 2000 Plasma Phys. Control. Fusion 42 731) and a synthetic diagnostic with the same geometry installed in RFX-mod. Good agreement between the synthetic and the experimental flow behaviour has been obtained, confirming that the flow oscillations observed with the associated convective cells are a signature of helical flow.

Physics of GAM-initiated L-H transition in a tokamak

NASA Astrophysics Data System (ADS)

Askinazi, L. G.; Belokurov, A. A.; Bulanin, V. V.; Gurchenko, A. D.; Gusakov, E. Z.; Kiviniemi, T. P.; Lebedev, S. V.; Kornev, V. A.; Korpilo, T.; Krikunov, S. V.; Leerink, S.; Machielsen, M.; Niskala, P.; Petrov, A. V.; Tukachinsky, A. S.; Yashin, A. Yu; Zhubr, N. A.

2017-01-01

Based on experimental observations using the TUMAN-3M and FT-2 tokamaks, and the results of gyrokinetic modeling of the interplay between turbulence and the geodesic acoustic mode (GAM) in these installations, a simple model is proposed for the analysis of the conditions required for L-H transition triggering by a burst of radial electric field oscillations in a tokamak. In the framework of this model, one-dimensional density evolution is considered to be governed by an anomalous diffusion coefficient dependent on radial electric field shear. The radial electric field is taken as the sum of the oscillating term and the quasi-stationary one determined by density and ion temperature gradients through a neoclassical formula. If the oscillating field parameters (amplitude, frequency, etc) are properly adjusted, a transport barrier forms at the plasma periphery and sustains after the oscillations are switched off, manifesting a transition into the high confinement mode with a strong inhomogeneous radial electric field and suppressed transport at the plasma edge. The electric field oscillation parameters required for L-H transition triggering are compared with the GAM parameters observed at the TUMAN-3M (in the discharges with ohmic L-H transition) and FT-2 tokamaks (where no clear L-H transition was observed). It is concluded based on this comparison that the GAM may act as a trigger for the L-H transition, provided that certain conditions for GAM oscillation and tokamak discharge are met.

Modeling Dust Emission of HL Tau Disk Based on Planet-Disk Interactions

DOE PAGES

Jin, Sheng; Li, Shengtai; Isella, Andrea; ...

2016-02-09

In this paper, we use extensive global two-dimensional hydrodynamic disk gas+dust simulations with embedded planets, coupled with three-dimensional radiative transfer calculations, to model the dust ring and gap structures in the HL Tau protoplanetary disk observed with the Atacama Large Millimeter/Submillimeter Array (ALMA). We include the self-gravity of disk gas and dust components and make reasonable choices of disk parameters, assuming an already settled dust distribution and no planet migration. We can obtain quite adequate fits to the observed dust emission using three planets with masses of 0.35, 0.17, and 0.26 M Jup at 13.1, 33.0, and 68.6 AU, respectively.more » Finally, implications for the planet formation as well as the limitations of this scenario are discussed.« less

Inductive flux usage and its optimization in tokamak operation

DOE PAGES

Luce, Timothy C.; Humphreys, David A.; Jackson, Gary L.; ...

2014-07-30

The energy flow from the poloidal field coils of a tokamak to the electromagnetic and kinetic stored energy of the plasma are considered in the context of optimizing the operation of ITER. The goal is to optimize the flux usage in order to allow the longest possible burn in ITER at the desired conditions to meet the physics objectives (500 MW fusion power with energy gain of 10). A mathematical formulation of the energy flow is derived and applied to experiments in the DIII-D tokamak that simulate the ITER design shape and relevant normalized current and pressure. The rate ofmore » rise of the plasma current was varied, and the fastest stable current rise is found to be the optimum for flux usage in DIII-D. A method to project the results to ITER is formulated. The constraints of the ITER poloidal field coil set yield an optimum at ramp rates slower than the maximum stable rate for plasmas similar to the DIII-D plasmas. Finally, experiments in present-day tokamaks for further optimization of the current rise and validation of the projections are suggested.« less

Sustained high βN plasmas on EAST tokamak

NASA Astrophysics Data System (ADS)

Gao, Xiang; the EAST team

2018-05-01

Sustained high normalized beta (βN ∼ 1.9) plasmas with an ITER-like tungsten divertor have been achieved on EAST tokamak recently. The high power NBI heating system of 4.8 MW and the 4.6 GHz lower hybrid wave of 1 MW were developed and applied to produce edge and internal transport barriers in high βN discharges. The central flat q profile with q (ρ) ∼ 1 at ρ < 0.3 region and edge safety factor q95 = 4.7 is identified by the multi-channel far-infrared laser polarimeter and the EFIT code. The fraction of non-inductive current is about 40%. The relation between fishbone activity and ITB formation is observed and discussed.

Stainless steel blanket concept for tokamaks

DOE Office of Scientific and Technical Information (OSTI.GOV)

Karbowski, J.S.; Lee, A.Y.; Prevenslik, T.V.

1979-01-25

The purpose of this joint ORNL/Westinghouse Program is to develop a design concept for a tokamak reactor blanket system which satisfies engineering requirements for a utility environment. While previous blanket studies have focused primarily on performance issues (thermal, neutronic, and structural), this study has emphasized consideration of reliability, fabricability, and lifetime.

Cytotoxic and Apoptotic Effects of Different Extracts of Artemisia turanica Krasch. on K562 and HL-60 Cell Lines

PubMed Central

Tayarani-Najaran, Zahra; Sareban, Mahla; Gholami, Atefeh; Emami, Seyed Ahmad; Mojarrab, Mahdi

2013-01-01

Artemisia is an important genus of Iranian flora. Cytotoxic activities for some species of the genus have already been reported. In this study, we have investigated the cytotoxic effects of n-hexane, CH2Cl2, EtOAc, EtOH, and EtOH/H2O (1 : 1) extracts of A. turanica Krasch. on two human leukemic cancer cell lines (K562 and HL-60) and J774 as normal cells using alamarBlue (resazurin) assay. PI staining of the fragmented DNA and western blot analysis were used to evaluate the possible apoptotic effect of the extract. The CH2Cl2 extract of A. turanica showed the most antiproliferative effect on cancer cells among all tested extracts with IC50 values of 69 and 104 μg/mL on K562 and HL-60 cells, respectively, whereas the normal cells were not affected significantly by this extract. Sub-G1 peak in the flow cytometry histogram of the cells treated with CH2Cl2 extract of A. turanica and cleavage of PARP protein confirmed the induction of apoptosis with CH2Cl2 extract. Taken together, the findings of the present work suggest the anticancer potential of CH2Cl2 extract of A. turanica on human leukemic cancer cell lines. PMID:24288497

The radiation asymmetry in MGI rapid shutdown on J-TEXT tokamak

NASA Astrophysics Data System (ADS)

Tong, Ruihai; Chen, Zhongyong; Huang, Duwei; Cheng, Zhifeng; Zhang, Xiaolong; Zhuang, Ge; J-TEXT Team

2017-10-01

Disruptions, the sudden termination of tokamak fusion plasmas by instabilities, have the potential to cause severe material wall damage to large tokamaks like ITER. The mitigation of disruption damage is an essential part of any fusion reactor system. Massive gas injection (MGI) rapid shutdown is a technique in which large amounts of noble gas are injected into the plasma in order to safely radiate the plasma energy evenly over the entire plasma-facing first wall. However, the radiated energy during the thermal quench (TQ) in massive gas injection (MGI) induced disruptions is found toroidal asymmetric, and the degrees of asymmetry correlate with the gas penetration and MGI induced magnetohydrodynamics (MHD) activities. A toroidal and poloidal array of ultraviolet photodiodes (AXUV) has been developed to investigate the radiation asymmetry on J-TEXT tokamak. Together with the upgraded mirnov probe arrays, the relation between MGI triggered MHD activities with radiation asymmetry is studied.

A fission-fusion hybrid reactor in steady-state L-mode tokamak configuration with natural uranium

DOE Office of Scientific and Technical Information (OSTI.GOV)

Reed, Mark; Parker, Ronald R.; Forget, Benoit

2012-06-19

This work develops a conceptual design for a fusion-fission hybrid reactor operating in steady-state L-mode tokamak configuration with a subcritical natural or depleted uranium pebble bed blanket. A liquid lithium-lead alloy breeds enough tritium to replenish that consumed by the D-T fusion reaction. The fission blanket augments the fusion power such that the fusion core itself need not have a high power gain, thus allowing for fully non-inductive (steady-state) low confinement mode (L-mode) operation at relatively small physical dimensions. A neutron transport Monte Carlo code models the natural uranium fission blanket. Maximizing the fission power gain while breeding sufficient tritiummore » allows for the selection of an optimal set of blanket parameters, which yields a maximum prudent fission power gain of approximately 7. A 0-D tokamak model suffices to analyze approximate tokamak operating conditions. This fission blanket would allow the fusion component of a hybrid reactor with the same dimensions as ITER to operate in steady-state L-mode very comfortably with a fusion power gain of 6.7 and a thermal fusion power of 2.1 GW. Taking this further can determine the approximate minimum scale for a steady-state L-mode tokamak hybrid reactor, which is a major radius of 5.2 m and an aspect ratio of 2.8. This minimum scale device operates barely within the steady-state L-mode realm with a thermal fusion power of 1.7 GW. Basic thermal hydraulic analysis demonstrates that pressurized helium could cool the pebble bed fission blanket with a flow rate below 10 m/s. The Brayton cycle thermal efficiency is 41%. This reactor, dubbed the Steady-state L-mode non-Enriched Uranium Tokamak Hybrid (SLEUTH), with its very fast neutron spectrum, could be superior to pure fission reactors in terms of breeding fissile fuel and transmuting deleterious fission products. It would likely function best as a prolific plutonium breeder, and the plutonium it produces could actually be more

A fission-fusion hybrid reactor in steady-state L-mode tokamak configuration with natural uranium

NASA Astrophysics Data System (ADS)

Reed, Mark; Parker, Ronald R.; Forget, Benoit

2012-06-01

This work develops a conceptual design for a fusion-fission hybrid reactor operating in steady-state L-mode tokamak configuration with a subcritical natural or depleted uranium pebble bed blanket. A liquid lithium-lead alloy breeds enough tritium to replenish that consumed by the D-T fusion reaction. The fission blanket augments the fusion power such that the fusion core itself need not have a high power gain, thus allowing for fully non-inductive (steady-state) low confinement mode (L-mode) operation at relatively small physical dimensions. A neutron transport Monte Carlo code models the natural uranium fission blanket. Maximizing the fission power gain while breeding sufficient tritium allows for the selection of an optimal set of blanket parameters, which yields a maximum prudent fission power gain of approximately 7. A 0-D tokamak model suffices to analyze approximate tokamak operating conditions. This fission blanket would allow the fusion component of a hybrid reactor with the same dimensions as ITER to operate in steady-state L-mode very comfortably with a fusion power gain of 6.7 and a thermal fusion power of 2.1 GW. Taking this further can determine the approximate minimum scale for a steady-state L-mode tokamak hybrid reactor, which is a major radius of 5.2 m and an aspect ratio of 2.8. This minimum scale device operates barely within the steady-state L-mode realm with a thermal fusion power of 1.7 GW. Basic thermal hydraulic analysis demonstrates that pressurized helium could cool the pebble bed fission blanket with a flow rate below 10 m/s. The Brayton cycle thermal efficiency is 41%. This reactor, dubbed the Steady-state L-mode non-Enriched Uranium Tokamak Hybrid (SLEUTH), with its very fast neutron spectrum, could be superior to pure fission reactors in terms of breeding fissile fuel and transmuting deleterious fission products. It would likely function best as a prolific plutonium breeder, and the plutonium it produces could actually be more