Surgical correction of the snout suffocation syndrome.

PubMed

Keipper, V L; Chikes, P G

1990-04-01

Two nursing home patients with difficulty breathing because of occlusion of the mouth and nares by an involuntary snout reflex-like mannerism have previously been described. In both cases advanced dementia, edentia, and a downward-angled nasal base were present. Life-threatening hypoxia occurred intermittently in one case, and after becoming severe, was corrected by the surgical procedure described.

Aspects of embryonic and larval development in bighead carp Hypophthalmichthys nobilis and silver carp Hypophthalmichthys molitrix

USGS Publications Warehouse

George, Amy E.; Chapman, Duane C.

2013-01-01

As bighead carp Hypophthalmichthys nobilis and silver carp H. molitrix (the bigheaded carps) are poised to enter the Laurentian Great Lakes and potentially damage the region’s economically important fishery, information on developmental rates and behaviors of carps is critical to assessing their ability to establish sustainable populations within the Great Lakes basin. In laboratory experiments, the embryonic and larval developmental rates, size, and behaviors of bigheaded carp were tracked at two temperature treatments, one “cold” and one “warm”. Developmental rates were computed using previously described stages of development and the cumulative thermal unit method. Both species have similar thermal requirements, with a minimum developmental temperature for embryonic stages of 12.1° C for silver carp and 12.9° C for bighead carp, and 13.3° C for silver carp larval stages and 13.4° C for bighead carp larval stages. Egg size differed among species and temperature treatments, as egg size was larger in bighead carp, and “warm" temperature treatments. The larvae started robust upwards vertical swimming immediately after hatching, interspersed with intervals of sinking. Vertical swimming tubes were used to measure water column distribution, and ascent and descent rates of vertically swimming fish. Water column distribution and ascent and descent rates changed with ontogeny. Water column distribution also showed some diel periodicity. Developmental rates, size, and behaviors contribute to the drift distance needed to fulfill the early life history requirements of bigheaded carps and can be used in conjunction with transport information to assess invasibility of a river.

Comparison of size, terminal fall velocity, and density of bighead carp, silver carp, and grass carp eggs for use in drift modeling

USGS Publications Warehouse

George, Amy E.; Garcia, Tatiana; Chapman, Duane C.

2017-01-01

Invasive Asian carp established in the United States spawn in the turbulent water of rivers, and their eggs and early larvae develop while drifting in the current. The eggs, which are believed to perish if they settle before hatching, are slightly denser than water and are held in suspension by water turbulence. It is possible to use egg drift modeling to assess the capability of a river to support the survival of Asian carp eggs. Detection of spawning and estimation of egg abundance in the drift are typically assessed by ichthyoplankton trawls. Correct sampling design and interpretation of trawl data require knowledge of the vertical distribution of eggs in the drift, which can be accomplished with particle transport models. Data that are required to populate models of egg drift and vertical distribution include physical properties of assessed rivers and information on egg size, density, and terminal fall velocity, but data on these egg characteristics have not been previously available. Physical characteristics of the eggs are presented as a function of postfertilization time. We recorded mean egg diameter and terminal fall velocity for eggs from each Asian carp species during the first 5 h of development and at approximately 12 and 22 h postfertilization. Eggs of all species reached their maximum size before 4 h. Water-hardened eggs of Silver Carp Hypophthalmichthys molitrix and Grass Carp Ctenopharyngodon idella were similarly sized in our trials, and water-hardened eggs of Bighead Carp H. nobilis were the largest. After water hardening, Silver Carp eggs sank slowest, and Bighead Carp eggs sank fastest. For a given species, smaller-diameter eggs generally had faster terminal fall velocities and higher specific gravity than larger eggs. We provide regression models of egg density and diameter for all three species, discuss usage of these data in modeling the drift and dispersion of Asian carp eggs, and discuss implications for egg sampling design.

Establishment and characterization of a fin cell line from blunt snout bream, Megalobrama amblycephala.

PubMed

Zhu, Dong-Mei; Yang, Kun; Wang, Wei-Min; Song, Wen

2013-12-01

This study established and characterized a new cell line (MAF) from the fin of blunt snout bream (Megalobrama amblycephala), a freshwater fish cultivated in China. MAF cells proliferated well in medium 199 supplemented with 10 % fetal bovine serum at 28 °C and have been subcultured more than 95 times in almost a year. MAF cells were revived at 90-95 % viability after 3-6 months of storage in liquid nitrogen. Karyotyping indicated that the modal chromosome number of MAF cells was 48. The MAF cell line consisted predominantly of fibroblastic and epithelial-like cells from M. amblycephala, which was confirmed by immunofluorescence and mitochondrial 12s rRNA sequencing. Viral susceptibility tests showed that MAF cells were susceptible to infection by snakehead rhabdovirus, spring viremia carp virus, and channel catfish virus, which was demonstrated by the presence of cytopathic effect, high viral titers, and PCR products. Bacterial cytotoxicity studies showed that extracellular products from Aeromonas hydrophila were toxic to MAF cells. Cu²⁺ was also cytotoxic to MAF cells, and the 24-h IC₅₀ value was 144.48 μmol/l. When MAF cells were transfected with pEGFP-N1 plasmid, bright fluorescent signals were observed, and the transfection efficiency reached up to 5 %. These results suggest that the MAF cell line may provide a valuable tool for studying virus pathogenesis, as well as cytotoxicity testing and genetic manipulation studies.

Characterization and Comparative Profiling of MiRNA Transcriptomes in Bighead Carp and Silver Carp

PubMed Central

Chi, Wei; Tong, Chaobo; Gan, Xiaoni; He, Shunping

2011-01-01

MicroRNAs (miRNAs) are small non-coding RNA molecules that are processed from large ‘hairpin’ precursors and function as post-transcriptional regulators of target genes. Although many individual miRNAs have recently been extensively studied, there has been very little research on miRNA transcriptomes in teleost fishes. By using high throughput sequencing technology, we have identified 167 and 166 conserved miRNAs (belonging to 108 families) in bighead carp (Hypophthalmichthys nobilis) and silver carp (Hypophthalmichthys molitrix), respectively. We compared the expression patterns of conserved miRNAs by means of hierarchical clustering analysis and log2 ratio. Results indicated that there is not a strong correlation between sequence conservation and expression conservation, most of these miRNAs have similar expression patterns. However, high expression differences were also identified for several individual miRNAs. Several miRNA* sequences were also found in our dataset and some of them may have regulatory functions. Two computational strategies were used to identify novel miRNAs from un-annotated data in the two carps. A first strategy based on zebrafish genome, identified 8 and 22 novel miRNAs in bighead carp and silver carp, respectively. We postulate that these miRNAs should also exist in the zebrafish, but the methodologies used have not allowed for their detection. In the second strategy we obtained several carp-specific miRNAs, 31 in bighead carp and 32 in silver carp, which showed low expression. Gain and loss of family members were observed in several miRNA families, which suggests that duplication of animal miRNA genes may occur through evolutionary processes which are similar to the protein-coding genes. PMID:21858165

Catalogue of snout mites (Acariformes: Bdellidae) of the world

USDA-ARS?s Scientific Manuscript database

Bdellidae (Trombidiformes: Prostigmata) are moderate to large sized predatory mites that inhabit soil, leaves, leaf litter, and intertidal rocks. They are readily recognized by an elongated, snout-like gnathosoma and by elbowed pedipalps bearing two (one in Monotrichobdella) long terminal setae. Des...

Getting Hooked on Physics!

ERIC Educational Resources Information Center

McHugh, M.; McCauley, V.

2016-01-01

A number of papers in this journal have dealt with the idea of using hooks in physics instruction. A hook, as the name suggests, engages students in learning by triggering their attention and interest. Hooks can be any type of pedagogical approach--a question, a demonstration, a puzzle or video. They are generally short and center on the most…

Utility of snout wipe samples for influenza A virus surveillance in exhibition swine populations

PubMed Central

Edwards, Jody L; Nelson, Sarah W; Workman, Jeffrey D; Slemons, Richard D; Szablewski, Christine M; Nolting, Jacqueline M; Bowman, Andrew S

2014-01-01

Background Sporadic influenza A virus (IAV) outbreaks in humans and swine have resulted from commingling of large numbers of people and pigs at agricultural fairs in the United States. Current antemortem IAV surveillance strategies in swine require collecting nasal swabs, which entails restraining pigs with snares. Restraint is labor-intensive for samplers, stressful for pigs, and displeasing to onlookers because pigs often resist and vocalize. Objective To evaluate the utility of snout wipes in exhibition swine as a method to make IAV surveillance efforts less intrusive, less labor-intensive, and more widely accepted among pig owners and exhibition officials. Methods Three materials (rayon/polyester gauze, cotton gauze, and Swiffer® Sweeper dry cloths) were inoculated with IAV, and viral recoveries from these materials were quantified using qRT-PCR and TCID50 assays. In a field trial, paired cotton gauze snout wipes and gold standard polyester-tipped nasal swabs were collected from 553 pigs representing 29 agricultural fairs and the qualitative results of rRT-PCR and viral isolation were compared. Results and Conclusions Viral recoveries from potential snout wipe materials ranged from 0·26 to 1·59 log10 TCID50/ml less than that of the positive control in which no substrate was included; rayon/polyester gauze performed significantly worse than the other materials. In the field, snout wipes and nasal swabs had high levels of agreement for both rRT-PCR detection and virus isolation. Although further investigation and refinement of the sampling method is needed, results indicate that snout wipes will facilitate convenient and undisruptive IAV surveillance in pigs at agricultural fairs. PMID:25043408

Genetic mapping and QTL analysis for body weight in Jian carp ( Cyprinus carpio var. Jian) compared with mirror carp ( Cyprinus carpio L.)

NASA Astrophysics Data System (ADS)

Gu, Ying; Lu, Cuiyun; Zhang, Xiaofeng; Li, Chao; Yu, Juhua; Sun, Xiaowen

2015-05-01

We report the genetic linkage map of Jian carp ( Cyprinus carpio var. Jian). An F1 population comprising 94 Jian carp individuals was mapped using 254 microsatellite markers. The genetic map spanned 1 381.592 cM and comprised 44 linkage groups, with an average marker distance of 6.58 cM. We identified eight quantitative trait loci (QTLs) for body weight (BW) in seven linkage groups, explaining 12.6% to 17.3% of the phenotypic variance. Comparative mapping was performed between Jian carp and mirror carp ( Cyprinus carpio L.), which both have 50 chromosomes. One hundred and ninety-eight Jian carp marker loci were found in common with the mirror carp map, with 186 (93.94%) showing synteny. All 44 Jian carp linkage groups could be one-to-one aligned to the 44 mirror carp linkage groups, mostly sharing two or more common loci. Three QTLs for BW in Jian carp were conserved in mirror carp. QTL comparison suggested that the QTL confidence interval in mirror carp was more precise than the homologous interval in Jian carp, which was contained within the QTL interval in Jian carp. The syntenic relationship and consensus QTLs between the two varieties provide a foundation for genomic research and genetic breeding in common carp.

Role of the Dc domain of the bacterial hook protein FlgE in hook assembly and function

PubMed Central

Moriya, Nao; Minamino, Tohru; Ferris, Hedda U.; Morimoto, Yusuke V.; Ashihara, Masamichi; Kato, Takayuki; Namba, Keiichi

2013-01-01

The bacterial flagellar hook acts as a universal joint to smoothly transmit torque produced by the motor to the filament. The hook protein FlgE assembles into a 55 nm tubular structure with the help of the hook cap (FlgD). FlgE consists of four domains, D0, Dc, D1 and D2, arranged from the inner to the outer part of the tubular structure of the hook. The Dc domain contributes to the structural stability of the hook, but it is unclear how this Dc domain is responsible for the universal joint mechanism. Here, we carried out a deletion analysis of the FlgE Dc domain. FlgEΔ4/5 with deletion of residues 30 to 49 was not secreted into the culture media. FlgEΔ5 and FlgEΔ6 with deletions of residues 40 to 49 and 50 to 59, respectively, still formed hooks, allowing the export apparatus to export the hook-filament junction proteins FlgK and FlgL and flagellin FliC. However, these deletions inhibited the replacement of the FlgD hook cap by FlgK at the hook tip, thereby abolishing filament formation. Deletion of residues 50 to 59 significantly affected hook morphology. These results suggest that the Dc domain is responsible not only for hook assembly but also for FlgE export, the interaction with FlgK, and the polymorphic supercoiling mechanism of the hook. PMID:27493542

Can Soft Water Limit Bighead Carp and Silver Carp (Hypophthalmichthys spp.) Invasions?

EPA Science Inventory

There is concern that the non-native bighead carp (Hypophthalmichthys nobilis) and silver carp (H. molitrix), now found in many large rivers of the Mississippi River Basin, may spread to other regions. However, evidence suggests that their eggs may not be able to survive in soft ...

Response of Bighead Carp and Silver Carp to repeated water gun operation in an enclosed shallow pond

USGS Publications Warehouse

Romine, Jason G.; Jensen, Nathan; Parsley, Michael J.; Gaugush, Robert F.; Severson, Todd J.; Hatton, Tyson W.; Adams, Ryan F.; Gaikowski, Mark P.

2015-01-01

The Bighead Carp Hypophthalmichthys nobilis and Silver Carp H. molitrix are nonnative species that pose a threat to Great Lakes ecosystems should they advance into those areas. Thus, technologies to impede Asian carp movement into the Great Lakes are needed; one potential technology is the seismic water gun. We evaluated the efficacy of a water gun array as a behavioral deterrent to the movement of acoustic-tagged Bighead Carp and Silver Carp in an experimental pond. Behavioral responses were evaluated by using four metrics: (1) fish distance from the water guns (D); (2) spatial area of the fish's utilization distribution (UD); (3) persistence velocity (Vp); and (4) number of times a fish transited the water gun array. For both species, average D increased by 10 m during the firing period relative to the pre-firing period. During the firing period, the spatial area of use within the pond decreased. Carp were located throughout the pond during the pre-firing period but were concentrated in the north end of the pond during the firing period, thus reducing their UDs by roughly 50%. Overall, Vp decreased during the firing period relative to the pre-firing period, as fish movement became more tortuous and confined, suggesting that the firing of the guns elicited a change in carp behavior. The water gun array was partially successful at impeding carp movement, but some fish did transit the array. Bighead Carp moved past the guns a total of 78 times during the pre-firing period and 15 times during the firing period; Silver Carp moved past the guns 96 times during the pre-firing period and 13 times during the firing period. Although the water guns did alter carp behavior, causing the fish to move away from the guns, this method was not 100% effective as a passage deterrent.

The role of HIRA and maternal histones in sperm nucleus decondensation in the gibel carp and color crucian carp.

PubMed

Zhao, Zhan-Ke; Li, Wei; Wang, Meng-Yu; Zhou, Li; Wang, Jia-Lin; Wang, Yu-Feng

2011-02-01

The histone H3.3 chaperone HIRA is essential for chromatin assembly during male pronucleus formation in Drosophila. However, the role of HIRA during fertilization in vertebrates remains unclear. The gibel carp (Carassius auratus gibelio) is a unique gynogenetic crucian carp (gyno-carp). Heterologous sperm nuclei cannot decondense when incorporated in the egg, thus the eggs produce a clonal lineage of all females by typical gynogenesis. In contrast, after entering the egg, homologous sperm can undergo decondensation and sexual reproduction is activated, which may produce both female and male offspring. Therefore, this fish is a useful model for studying the mechanisms of fertilization. Herein, we first compared HIRA expression during embryogenesis between gyno-carp and the gonochoristic color crucian carp (Carassius auratus; gono-carp). In gono-carp, a dramatic reduction of HIRA protein occurs shortly after fertilization, whereas HIRA protein is consistently expressed during embryogenesis of gyno-carp. Next, we used immunodepletion and an in vitro sperm decondensation system, and found that complete removal of HIRA inhibited sperm decondensation in both of the fish. Immunofluorescence localization showed that in the condensed sperm nuclei of gono-carp incubated in gyno-carp egg extracts, HIRA was detected, but neither the histone H2A variant H2af1o nor acetylated histone H4 was observed. These results suggest that HIRA may be a critical factor required for sperm nucleus decondensation, while the defect in deposition of some maternal histones in the sperm nucleus could be one reason why heterologous sperm cannot decondense in the gibel carp egg. Copyright © 2011 Wiley-Liss, Inc.

Spring viremia of carp

USGS Publications Warehouse

Ahne, W.; Bjorklund, H.V.; Essbauer, S.; Fijan, N.; Kurath, G.; Winton, J.R.

2002-01-01

pring viremia of carp (SVC) is an important disease affecting cyprinids, mainly common carp Cyprinus carpio. The disease is widespread in European carp culture, where it causes significant morbidity and mortality. Designated a notifiable disease by the Office International des Epizooties, SVC is caused by a rhabdovirus, spring viremia of carp virus (SVCV). Affected fish show destruction of tissues in the kidney, spleen and liver, leading to hemorrhage, loss of water-salt balance and impairment of immune response. High mortality occurs at water temperatures of 10 to 17°C, typically in spring. At higher temperatures, infected carp develop humoral antibodies that can neutralize the spread of virus and such carp are protected against re-infection by solid immunity. The virus is shed mostly with the feces and urine of clinically infected fish and by carriers. Waterborne transmission is believed to be the primary route of infection, but bloodsucking parasites like leeches and the carp louse may serve as mechanical vectors of SVCV. The genome of SVCV is composed of a single molecule of linear, negative-sense, single-stranded RNA containing 5 genes in the order 3¹-NPMGL-5¹ coding for the viral nucleoprotein, phosphoprotein, matrix protein, glycoprotein, and polymerase, respectively. Polyacrylamide gel electrophoresis of the viral proteins, and sequence homologies between the genes and gene junctions of SVCV and vesicular stomatitis viruses, have led to the placement of the virus as a tentative member of the genus Vesiculovirus in the family Rhabdoviridae. These methods also revealed that SVCV is not related to fish rhabdoviruses of the genus Novirhabdovirus. In vitro replication of SVCV takes place in the cytoplasm of cultured cells of fish, bird and mammalian origin at temperatures of 4 to 31°C, with an optimum of about 20°C. Spring viremia of carp can be diagnosed by clinical signs, isolation of virus in cell culture and molecular methods. Antibodies directed

Stress analyses of B-52 pylon hooks

NASA Technical Reports Server (NTRS)

Ko, W. L.; Schuster, L. S.

1985-01-01

The NASTRAN finite element computer program was used in the two dimensional stress analysis of B-52 carrier aircraft pylon hooks: (1) old rear hook (which failed), (2) new rear hook (improved geometry), (3) new DAST rear hook (derated geometry), and (4) front hook. NASTRAN model meshes were generated by the aid of PATRAN-G computer program. Brittle limit loads for all the four hooks were established. The critical stress level calculated from NASTRAN agrees reasonably well with the values predicted from the fracture mechanics for the failed old rear hook.

Molecular responses differ between sensitive silver carp and tolerant bighead carp and bigmouth buffalo exposed to rotenone

USGS Publications Warehouse

Amberg, Jon J.; Schreier, Theresa M.; Gaikowski, Mark P.

2012-01-01

Some species of fish are more tolerant of rotenone, a commonly used non-specific piscicide, than others. This species-specific tolerance to rotenone has been thought to be associated with the uptake and the efficiency at which the chemical is detoxified. However, rotenone stimulates oxidative stress and superoxides, which are also toxic. Understanding the modes in which fish physiologically respond to rotenone is important in developing improved protocols for its application in controlling aquatic nuisance species. Using a molecular approach, we investigated the physiological and molecular mechanisms of rotenone resistance. Species-specific responses were observed when rotenone-sensitive silver, Hypophthalmichthys molitrix, and both rotenone-resistant bighead carp, Hypophthalmichthys nobilis, and bigmouth buffalo, Ictiobus cyprinellus, were exposed to rotenone. Rotenone levels in plasma were highest 90 min after exposure in both silver carp and bigmouth buffalo, but bigmouth buffalo tolerated over twice the burden (ng mL-1 g-1) than silver carp. Expression of genes related with detoxification (cyp1a and gst) increased in silver carp, but either decreased or remained the same in bighead carp. Genes linked with oxidative stress in the cytosol (gpx, cat and sod1) and hsp70 increased only in silver carp after a 6-h exposure. Expression of genes associated with oxidative stress in the mitochondria (sod2 and ucp2) differed between silver carp and bighead carp. Expression of sod2 changed minimally in bighead carp, but expression of ucp2 linearly increased to nearly 85-fold of the level prior to exposure. Expression of sod2 and ucp2 did not change until 6 h in silver carp. Use of sod1 and sod2 to combat oxidative stress results in hydrogen peroxide production, while use of ucp2 produces nitric oxide, a chemical known to inhibit apoptosis. We conclude that the mechanism at which a fish handles oxidative stress plays an important role in the tolerance to rotenone.

Survival of foul-hooked largemouth bass (Micropterus salmoides)

USGS Publications Warehouse

Pope, K.L.; Wilde, G.R.

2010-01-01

We conducted a field experiment to determine the survival rate of foul-hooked (hooked external to the oral cavity) largemouth bass (Micropterus salmoides) caught and released by recreational anglers. Of 42 largemouth bass caught with hard-plastic baits containing three treble hooks, 15 were hooked only within the mouth and 27 had at least one hook penetrating the external surface of the fish (i.e., foul-hooked). There was no difference in survival of mouth-hooked (100%), foul-hooked (100%), or control (100%) largemouth bass.

Getting Hooked on Physics!

NASA Astrophysics Data System (ADS)

McHugh, M.; McCauley, V.

2016-12-01

A number of papers in this journal have dealt with the idea of using hooks in physics instruction. A hook, as the name suggests, engages students in learning by triggering their attention and interest. Hooks can be any type of pedagogical approach—a question, a demonstration, a puzzle or video. They are generally short and center on the most interesting aspects of a topic. Here we focus on using Conceptual Change (CC) teacher-led demonstrations as the pedagogical hook approach. Conceptual Change is an instructional method mentioned by a number of authors to stimulate interest among learners. This is where an educator presents an issue or topic that has associated misconceptions. As the teacher explains the truth behind the concept, cognitive conflict occurs and the students' initial perceptions are challenged and ideas start to change. However, the instruction used in association with the demonstrations must provide intelligible, plausible, relevant explanations so that students are convinced by the new ideas. The gap between prior knowledge and new knowledge has the potential to provide a strong stimulus for augmenting interest, engagement, and attention among students. Thus, CC can act as a hook in itself.

Instability of hooks during bacterial flagellar swimming

NASA Astrophysics Data System (ADS)

Jabbarzadeh, Mehdi; Fu, Henry C.; Henry Fu Team

2016-11-01

In bacteria, a flexible hook transmits torque from the rotary motor at the cell body to the flagellum. Previously, the hook has been modeled as a Kirchhoff rod between the cell body and rotating flagellum. To study effects of the hook's flexibility on the bacteria's swimming speed and trajectory for wide range hook stiffnesses and flagellum configurations, we develop an efficient simplified spring model for the hook by linearizing the Kirchhoff rod. We treat the hydrodynamics of the cell body and helical flagellum using resistance matrices calculated by the method of regularized Stokeslets. We investigate flagellar and swimming dynamics for a range of hook flexibilities and flagellar orientations relative to the cell body and compare the results to models without hook flexibility. We investigate in detail parameters corresponding to E. coli and Vibrio alginolyticus. Generally, the flagellum changes orientation relative to the cell body, undergoing an orbit with the period of the motor rotation. We find that as the hook stiffness decreases, steady-state orbits of the flagellum first become unstable before the hook buckles, which may suggest a new mechanism of flick initiation in run-reverse-flick motility. We also find that for some parameter ranges, there are multiple stable steady state orbits, which may have implications for the tumbling and turning of bacteria.

Variable Spaced Grating (VSG) Snout, Rotator and Rails for use at LLE

DOE Office of Scientific and Technical Information (OSTI.GOV)

Mukherjee, S K; Emig, J A; Griffith, L V

2010-01-25

The Variable Spaced Grating (VSG) is a spectrometer snout mounted to an X-Ray Framing Camera (XRFC) through the Unimount flange. This equipment already exists and is used at the University of Rochester, Laboratory for Laser Energetics (LLE) facility. The XRFC and the Unimount flange are designed by LLE. The Tilt Rotator fixture that mounts next to the XRFC and the cart rails are designed by LLNL, and are included in this safety note. The other related components, such as the TIM rails and the Unimount flange, are addressed in a separate safety note, EDSN09-500005-AA. The Multipurpose Spectrometer (MSPEC) and VSGmore » are mounted on the TIM Boat through the cart rails that are very similar in design. The tilt rotator combination with the Unimount flange is also a standard mounting procedure. The later mounting system has been included in this safety note. Figure-1 shows the interface components and the VSG snout. Figure-2 shows the VSG assembly mounted on the Unimount flange. The calibration pointer attachment is shown in place of the snout. There are two types of VSG, one made of 6061-T6 aluminum, weighing approximately 3 pounds, and the other made of 304 stainless steel, weighing approximately 5.5 pounds. This safety note examines the VSG steel design. Specific experiments may require orienting the VSG snout in 90 degrees increment with respect to the Unimount flange. This is done by changing the bolts position on the VSG-main body adapter flange to the Unimount adapter plate. There is no hazard involved in handling the VSG during this procedure as it is done outside the target chamber on the cart rail before installing on the TIM. This safety note addresses the mechanical integrity of the VSG structure, the tilt rotating fixture, the cart rails with handle and their connections. Safety Factors are also calculated for the MSPEC in place of the VSG.« less

Snout and Visual Rooting Reflexes in Infantile Autism. Brief Report.

ERIC Educational Resources Information Center

Minderaa, Ruud B.; And Others

1985-01-01

The authors conducted extensive neurological evaluations of 42 autistic individuals and were surprised to discover a consistently positive snout reflex in most of them. Difficulties with assessing the reflex are noted. The authors then reassessed the Ss for a series of primitive reflexes which are interpreted as signs of diffuse cortical brain…

Emergence of carp edema virus (CEV) and its significance to European common carp and koi Cyprinus carpio.

PubMed

Way, K; Haenen, O; Stone, D; Adamek, M; Bergmann, S M; Bigarré, L; Diserens, N; El-Matbouli, M; Gjessing, M C; Jung-Schroers, V; Leguay, E; Matras, M; Olesen, N J; Panzarin, V; Piačková, V; Toffan, A; Vendramin, N; Vesel, T; Waltzek, T

2017-10-18

Carp edema virus disease (CEVD), also known as koi sleepy disease, is caused by a poxvirus associated with outbreaks of clinical disease in koi and common carp Cyprinus carpio. Originally characterised in Japan in the 1970s, international trade in koi has led to the spread of CEV, although the first recognised outbreak of the disease outside of Japan was not reported until 1996 in the USA. In Europe, the disease was first recognised in 2009 and, as detection and diagnosis have improved, more EU member states have reported CEV associated with disease outbreaks. Although the structure of the CEV genome is not yet elucidated, molecular epidemiology studies have suggested distinct geographical populations of CEV infecting both koi and common carp. Detection and identification of cases of CEVD in common carp were unreliable using the original PCR primers. New primers for conventional and quantitative PCR (qPCR) have been designed that improve detection, and their sequences are provided in this paper. The qPCR primers have successfully detected CEV DNA in archive material from investigations of unexplained carp mortalities conducted >15 yr ago. Improvement in disease management and control is possible, and the principles of biosecurity, good health management and disease surveillance, applied to koi herpesvirus disease, can be equally applied to CEVD. However, further research studies are needed to fill the knowledge gaps in the disease pathogenesis and epidemiology that, currently, prevent an accurate assessment of the likely impact of CEVD on European koi and common carp aquaculture and on wild carp stocks.

Genetic polymorphism and isoenzyme patterns of lactate dehydrogenase in tench (Tinca tinca), crucian carp (Carassius carassius) and carp (Cyprinus carpio).

PubMed

Valenta, M; Slechta, V; Slechtová, V; Kálal, L

1977-01-01

Isoenzyme patterns and the polymorphism of lactate dehydrogenase (LDH) were investigated in 3 fish species of family Cyprinidae, i.e. tench (Tinca tinca), crucian carp (Carassius carassius) and carp (Cyprinus carpio). The isoenzyme patterns were tissue and species specific. In crucian carp subunits with different electrophoretic mobility are present, which are genetically controlled from the B1, B2, A1, A2 and C loci, while the set of loci in carp is B1, B2, A, C1 and C2 and in tench B, A, C. The locus B of LDH in tench, the locus B2 in crucian carp, and the loci B1, C1 and C2 in carp are polymorphic and have two different alleles in each case. The polymorphism did not affect the total LDH activity in the tissues. All the populations investigated were in Hardy-Weinberg equilibrium. The genetic control of the polymorphism in B1 and C1 loci in carp was proved by test matings. The polymorphism in B loci tested in erythrocytes may be utilized as genetic markers in the fish breeding.

21 CFR 868.5420 - Ether hook.

Code of Federal Regulations, 2011 CFR

2011-04-01

... 21 Food and Drugs 8 2011-04-01 2011-04-01 false Ether hook. 868.5420 Section 868.5420 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN SERVICES (CONTINUED) MEDICAL DEVICES ANESTHESIOLOGY DEVICES Therapeutic Devices § 868.5420 Ether hook. (a) Identification. An ether hook is a device...

Use of eyeballs for establishing ploidy of Asian carp

USGS Publications Warehouse

Jenkins, J.A.; Thomas, R.G.

2007-01-01

Grass carp Ctenopharyngodon idella, silver carp Hypophthalmichthys molitrix, and bighead carp H. nobilis are now established and relatively common in the Mississippi and Atchafalaya rivers. Commercial fishers of Louisiana's large rivers report recurrent catches of grass carp, and the frequency of bighead carp and silver carp catch is increasing. Twelve black carp Mylopharyngodon piceus were recently captured from the Mississippi and Atchafalaya River system, and 10 were analyzed for ploidy. By using the methods described herein, all 10 fish were determined to be diploid. Such correct identifications of ploidy of feral Asian carp species, as well as other species, would provide science-based information constructive for meeting reporting requirements, tracking fish movements, and forecasting expansion of species distribution. To investigate the postmortem period for sample collection and to lessen demands on field operations for obtaining samples, a laboratory study was performed to determine the length of time for which eyeballs from postmortem black carp could be used for ploidy determinations. Acquiring eyes rather than blood is simpler and quicker and requires no special supplies. An internal DNA reference standard with a documented genome size, including erythrocytes from diploid black carp or Nile tilapia Oreochromis niloticus, was analyzed simultaneously with cells from seven known triploid black carp to assess ploidy through 12 d after extraction. Ploidy determinations were reliable through 8 d postmortem. The field process entails excision of an eyeball, storage in a physiological buffer, and shipment within 8 d at refrigeration temperatures (4??C) to the laboratory for analysis by flow cytometry. ?? Copyright by the American Fisheries Society 2007.

Subacromial morphometric assessment of the clavicle hook plate.

PubMed

ElMaraghy, Amr W; Devereaux, Moira W; Ravichandiran, Kajeandra; Agur, Anne M

2010-06-01

Clavicle hook plates are an effective plate fixation alternative for distal clavicle fractures and severe acromioclavicular joint dislocations. However, post-operative complications associated with the subacromial portion of the hook include acromial osteolysis and subacromial impingement. We examine and quantify the three-dimensional position of the subacromial portion of the hook plate relative to surrounding acromial and subacromial structures in a series of cadaveric shoulders to determine if hook positioning predisposes the shoulder to these noted post-operative complications. Fifteen cadaveric shoulders (seven males, eight females) were implanted with 15- or 18-mm hook plates. Dimensions of the acromion and hook plate were digitised and reconstructed into a three-dimensional model to measure acromion dimensions and distances of the subacromial hook relative to surrounding acromial and subacromial structures. Inter-specimen dimensions of the acromion were highly variable. Mean acromion width and thickness were greater in males than in females (p=0.01). The posterior orientation of the subacromial hook varied widely (mean posterior implantation angle=32.5+/-20 degrees, range 0-67 degrees). The hook pierced the subacromial bursa in 13/15 specimens, made contact with the belly of the supraspinatus muscle in 9/15 specimens, and had focal contact at the hook tip with the undersurface of the acromion in 9/15 specimens. The wide range of acromial dimensions leads to a high degree of variability in the positioning of the subacromial hook. The observed frequency of hook contact with surrounding subacromial structures in a static shoulder confirms that the position of the hook portion of the implant can predispose anatomic structures to the post-operative complications of subacromial impingement and bony erosion. Copyright 2009 Elsevier Ltd. All rights reserved.

Evidence for recombination of mitochondrial DNA in triploid crucian carp.

PubMed

Guo, Xinhong; Liu, Shaojun; Liu, Yun

2006-03-01

In this study, we report the complete mitochondrial DNA (mtDNA) sequences of the allotetraploid and triploid crucian carp and compare the complete mtDNA sequences between the triploid crucian carp and its female parent Japanese crucian carp and between the triploid crucian carp and its male parent allotetraploid. Our results indicate that the complete mtDNA nucleotide identity (98%) between the triploid crucian carp and its male parent allotetraploid was higher than that (93%) between the triploid crucian carp and its female parent Japanese crucian carp. Moreover, the presence of a pattern of identity and difference at synonymous sites of mitochondrial genomes between the triploid crucian carp and its parents provides direct evidence that triploid crucian carp possessed the recombination mtDNA fragment (12,759 bp) derived from the paternal fish. These results suggest that mtDNA recombination was derived from the fusion of the maternal and paternal mtDNAs. Compared with the haploid egg with one set of genome from the Japanese crucian carp, the diploid sperm with two sets of genomes from the allotetraploid could more easily make its mtDNA fuse with the mtDNA of the haploid egg. In addition, the triple hybrid nature of the triploid crucian carp probably allowed its better mtDNA recombination. In summary, our results provide the first evidence of mtDNA combination in polyploid fish.

29 CFR 1915.113 - Shackles and hooks.

Code of Federal Regulations, 2010 CFR

2010-07-01

... bends or springs the hook. (3) Hooks shall be inspected periodically to see that they have not been bent by overloading. Bent or sprung hooks shall not be used. [47 FR 16986, Apr. 20, 1982, as amended at 51...

29 CFR 1915.113 - Shackles and hooks.

Code of Federal Regulations, 2011 CFR

2011-07-01

... bends or springs the hook. (3) Hooks shall be inspected periodically to see that they have not been bent by overloading. Bent or sprung hooks shall not be used. [47 FR 16986, Apr. 20, 1982, as amended at 51...

Simulated population responses of common carp to commercial exploitation

DOE Office of Scientific and Technical Information (OSTI.GOV)

Weber, Michael J.; Hennen, Matthew J.; Brown, Michael L.

2011-12-01

Common carp Cyprinus carpio is a widespread invasive species that can become highly abundant and impose deleterious ecosystem effects. Thus, aquatic resource managers are interested in controlling common carp populations. Control of invasive common carp populations is difficult, due in part to the inherent uncertainty of how populations respond to exploitation. To understand how common carp populations respond to exploitation, we evaluated common carp population dynamics (recruitment, growth, and mortality) in three natural lakes in eastern South Dakota. Common carp exhibited similar population dynamics across these three systems that were characterized by consistent recruitment (ages 3 to 15 years present),more » fast growth (K = 0.37 to 0.59), and low mortality (A = 1 to 7%). We then modeled the effects of commercial exploitation on size structure, abundance, and egg production to determine its utility as a management tool to control populations. All three populations responded similarly to exploitation simulations with a 575-mm length restriction, representing commercial gear selectivity. Simulated common carp size structure modestly declined (9 to 37%) in all simulations. Abundance of common carp declined dramatically (28 to 56%) at low levels of exploitation (0 to 20%) but exploitation >40% had little additive effect and populations were only reduced by 49 to 79% despite high exploitation (>90%). Maximum lifetime egg production was reduced from 77 to 89% at a moderate level of exploitation (40%), indicating the potential for recruitment overfishing. Exploitation further reduced common carp size structure, abundance, and egg production when simulations were not size selective. Our results provide insights to how common carp populations may respond to exploitation. Although commercial exploitation may be able to partially control populations, an integrated removal approach that removes all sizes of common carp has a greater chance of controlling population

Experimental Hydrodynamics of Turning Maneuvers in Koi Carps

NASA Astrophysics Data System (ADS)

Wu, G. H.; Yang, Y.; Zeng, L. J.

Experimental hydrodynamics of two types of turning maneuvers in koi carps (cyprinus carpio koi) are studied. The flow patterns generated by koi carps during turning are quantified by using digital particle image velocimetry. Based on the velocity fields measured, the momentums in the wake and the impulsive moments exerted on the carps are estimated. On the other hand, turning rates and radii, and moments of inertia of the carps including added mass during turning are obtained by processing the images recorded. Comparisons of the impulsive moments and moments of inertia show good agreements.

Experimental infections of different carp strains with the carp edema virus (CEV) give insights into the infection biology of the virus and indicate possible solutions to problems caused by koi sleepy disease (KSD) in carp aquaculture.

PubMed

Adamek, Mikolaj; Oschilewski, Anna; Wohlsein, Peter; Jung-Schroers, Verena; Teitge, Felix; Dawson, Andy; Gela, David; Piackova, Veronika; Kocour, Martin; Adamek, Jerzy; Bergmann, Sven M; Steinhagen, Dieter

2017-02-21

Outbreaks of koi sleepy disease (KSD) caused by carp edema virus (CEV) may seriously affect populations of farmed common carp, one of the most important fish species for global food production. The present study shows further evidence for the involvement of CEV in outbreaks of KSD among carp and koi populations: in a series of infection experiments, CEV from two different genogroups could be transmitted to several strains of naïve common carp via cohabitation with fish infected with CEV. In recipient fish, clinical signs of KSD were induced. The virus load and viral gene expression results confirm gills as the target organ for CEV replication. Gill explants also allowed for a limited virus replication in vitro. The in vivo infection experiments revealed differences in the virulence of the two CEV genogroups which were associated with infections in koi or in common carp, with higher virulence towards the same fish variety as the donor fish. When the susceptibility of different carp strains to a CEV infection and the development of KSD were experimentally investigated, Amur wild carp showed to be relatively more resistant to the infection and did not develop clinical signs for KSD. However, the resistance could not be related to a higher magnitude of type I IFN responses of affected tissues. Despite not having a mechanistic explanation for the resistance of Amur wild carp to KSD, we recommend using this carp strain in breeding programs to limit potential losses caused by CEV in aquaculture.

Acoustical deterrence of Silver Carp (Hypophthalmichthys molitrix)

USGS Publications Warehouse

Brooke J. Vetter,; Cupp, Aaron R.; Fredricks, Kim T.; Gaikowski, Mark P.; Allen F. Mensinger,

2015-01-01

The invasive Silver Carp (Hypophthalmichthys molitrix) dominate large regions of the Mississippi River drainage and continue to expand their range northward threatening the Laurentian Great Lakes. This study found that complex broadband sound (0–10 kHz) is effective in altering the behavior of Silver Carp with implications for deterrent barriers or potential control measures (e.g., herding fish into nets). The phonotaxic response of Silver Carp was investigated using controlled experiments in outdoor concrete ponds (10 × 4.9 × 1.2 m). Pure tones (500–2000 Hz) and complex sound (underwater field recordings of outboard motors) were broadcast using underwater speakers. Silver Carp always reacted to the complex sounds by exhibiting negative phonotaxis to the sound source and by alternating speaker location, Silver Carp could be directed consistently, up to 37 consecutive times, to opposite ends of the large outdoor pond. However, fish habituated quickly to pure tones, reacting to only approximately 5 % of these presentations and never showed more than two consecutive responses. Previous studies have demonstrated the success of sound barriers in preventing Silver Carp movement using pure tones and this research suggests that a complex sound stimulus would be an even more effective deterrent.

"Hooking up" among college students: demographic and psychosocial correlates.

PubMed

Owen, Jesse J; Rhoades, Galena K; Stanley, Scott M; Fincham, Frank D

2010-06-01

This study investigated 832 college students' experiences with hooking up, a term that refers to a range of physically intimate behavior (e.g., passionate kissing, oral sex, and intercourse) that occurs outside of a committed relationship. Specifically, we examined how five demographic variables (sex, ethnicity, parental income, parental divorce, and religiosity) and six psychosocial factors (e.g., attachment styles, alcohol use, psychological well-being, attitudes about hooking up, and perceptions of the family environment) related to whether individuals had hooked up in the past year. Results showed that similar proportions of men and women had hooked up but students of color were less likely to hook up than Caucasian students. More alcohol use, more favorable attitudes toward hooking up, and higher parental income were associated with a higher likelihood of having hooked up at least once in the past year. Positive, ambivalent, and negative emotional reactions to the hooking up experience(s) were also examined. Women were less likely to report that hooking up was a positive emotional experience than men. Young adults who reported negative and ambivalent emotional reactions to hooking up also reported lower psychological well-being and less favorable attitudes toward hooking up as compared to students who reported a positive hooking up experience. Based on these findings, suggestions for psychoeducational programming are offered. Additionally, directions for future research are provided.

Mechanics of plant fruit hooks

PubMed Central

Chen, Qiang; Gorb, Stanislav N.; Gorb, Elena; Pugno, Nicola

2013-01-01

Hook-like surface structures, observed in some plant species, play an important role in the process of plant growth and seed dispersal. In this study, we developed an elastic model and further used it to investigate the mechanical behaviour of fruit hooks in four plant species, previously measured in an experimental study. Based on Euler–Bernoulli beam theory, the force–displacement relationship is derived, and its Young's modulus is obtained. The result agrees well with the experimental data. The model aids in understanding the mechanics of hooks, and could be used in the development of new bioinspired Velcro-like materials. PMID:23365190

Robert Hooke, 1635-1703.

PubMed

Rowbury, Robin

2012-01-01

Robert Hooke was a polymath whose expertise during the 17th century spanned many different scientific areas. As a schoolboy on the Isle of Wight he was obsessed with the possibility of human flight and later became equally absorbed in cosmology and planetary motion. His skills as an artist were put to good use both as an architect following the Great Fire of London and before that in Micrographia. Although that book is best known for demonstrating the power of Hooke's microscope, Micrographia describes distant planetary bodies, the wave theory of light, the organic origin of fossils, and various other philosophical and scientific interests of its author The following thumbnail sketches of Hooke reveal him to be a man of enormous energy and imagination whose ideas were often pirated or under-rated.

Histopathological changes caused by the metacestodes of Neogryporhynchus cheilancristrotus (Wedl, 1855) in the gut of the gibel carp, Carassius gibelio.

PubMed

Molnár, K

2005-01-01

Metacestodes of Neogryporhynchus cheilancristrotus (Wedl, 1855) were found in the gut of some gibel carp (Carassius gibelio) specimens from a Hungarian water reservoir. Location of metacestodes in the freshly opened gut was marked with disseminated, red-coloured, pinhead-sized nodules in the anterior part of the intestine. In histological sections, metacestodes were found in a hole inside the propria layer of the intestinal folds. The worms were in direct contact with the host tissue without being encapsulated as a result of host reaction. In some specimens with extruded rostellum the rostellar hooks were bored into the host tissue and suckers grabbed pieces of the surrounding connective tissue. Around the worms, congested capillaries and formation of macrophages were seen in the lysed connective tissue.

Synthetic Minor NSR Permit: Van Hook Crude Terminal, LLC - Van Hook Crude Terminal/Rail Loading Facility

EPA Pesticide Factsheets

This page contains the current effective synthetic minor NSR permit for the Van Hook Crude Terminal, LLC, Van Hook Crude Terminal/Rail Loading Facility, located on the Fort Berthold Indian Reservation in Mountrail County, ND.

Invasional meltdown in northern lakes: Common carp invasion ...

EPA Pesticide Factsheets

Disturbances can lead to nonrandom changes in community composition due to interactions between the disturbance and the characteristics of species found in the community or available to colonize, producing both winners and losers of disturbance. When the disturbance is a biological invasion, it has been proposed that other nonnative species may be facilitated, producing positive feedbacks that drive an “invasional meltdown.” We investigated this phenomenon in Minnesota, where 100+ years of Cyprinus carpio (common carp) invasion have fundamentally altered the condition of many lakes. Common carp disturb macrophytes through foraging and bioturbation that causes nutrient loading and low water clarity. We evaluated effects of common carp on lake plant communities and tested whether carp were associated with increased occurrence of nonnative plant species. We hypothesized that there would be strong shifts in plant community composition associated with carp invasion and that plant species would be differentially sensitive to carp, with nonnative plant species more likely to be tolerant. We tested these hypotheses using vegetation, fish, and environmental data collected from 913 lakes over 20 years (1993–2012). This work describes an analysis of the effects of carp invasion on aquatic plant communities in glacial lakes. The results will provide a historical perspective on ecosystem effects of this invasive species that will inform management of aquatic plants, c

Predator-induced morphology enhances escape locomotion in crucian carp.

PubMed

Domenici, Paolo; Turesson, Håkan; Brodersen, Jakob; Brönmark, Christer

2008-01-22

Fishes show a remarkable diversity of shapes which have been associated with their swimming abilities and anti-predator adaptations. The crucian carp (Carassius carassius) provides an extreme example of phenotypic plasticity in body shape which makes it a unique model organism for evaluating the relationship between body form and function in fishes. In crucian carp, a deep body is induced by the presence of pike (Esox lucius), and this results in lower vulnerability to gape-limited predators, such as pike itself. Here, we demonstrate that deep-bodied crucian carp attain higher speed, acceleration and turning rate during anti-predator responses than shallow-bodied crucian carp. Therefore, a predator-induced morphology in crucian carp enhances their escape locomotor performance. The deep-bodied carp also show higher percentage of muscle mass. Therefore, their superior performance in escape swimming may be due to a combination of higher muscle power and higher thrust.

Designing physics video hooks for science students

NASA Astrophysics Data System (ADS)

McHugh, M.; McCauley, V.

2016-01-01

This paper offers an insight into the design structure of physics video hooks that were developed by the Science Education Resource design team in the school of education (SOE) in National University of Ireland, Galway (NUI Galway). A hook, is an instructional technique used to stimulate student attention (Hunter 1994, Lemov 2010), interest (Jewett 2013) and engagement (McCrory 2011, Riendeau 2013). The physics video hooks followed a design framework that is illustrated below by breaking down the centre of gravity (COG) hook. Various design principles and elements embedded within the COG hook are presented with examples and the time they occur within the video. The intention of this article is that the design can be replicated and modified to aid teachers and designers in the development of a multitude of classroom based multimedia resources.

Performance of barbed and barbless hooks in a marine recreational fishery

USGS Publications Warehouse

Schaeffer, Jeffrey S.; Hoffman, Elizabeth M.

2002-01-01

We used an angling study to examine catch per unit effort (CPUE), bait loss, and total landings by anglers fishing with natural bait on barbed and barbless hooks in a nearshore marine sport fishery located in the Gulf of Mexico near St. Petersburg, Florida. Anglers fished half the day with a barbed hook and half the day with a barbless hook. We also recorded anatomical hook placement, severity of injury or bleeding, and hook extraction times for each landed fish. Bait loss, CPUE, and mean length of catch did not differ between gears, but anglers landed 22% more fish with barbed hooks. Loss of hooked fish was significantly higher with barbless hooks, and efficiency appeared to vary among species. Mean unhooking times were significantly shorter with barbless hooks. Anatomical hook placement did not differ between gears and most fish were hooked in the jaws. Bleeding did not differ between gears because bleeding was influenced strongly by hook placement, but barbless hooks reduced unhooking injuries. In this fishery, barbless hooks probably did not reduce hooking mortality and conferred only slight benefits at the expense of reduced catches.

Carp vitellogenin detection by an optical waveguide lightmode spectroscopy biosensor.

PubMed

Kim, Namsoo; Kim, Dong-Kyung; Cho, Yong-Jin; Moon, Dae-Kyung; Kim, Woo-Yeon

2008-11-15

A label-free carp vitellogenin sensor has a strong potential for on-site monitoring on the possible contamination of edible fish with endocrine disruptors as a sum parameter in an inland carp farm. In this study, we performed a sensitive detection for carp vitellogenin with a direct-binding optical waveguide lightmode spectroscopy-based immunosensor. Carp vitellogenin bound over the sensor surface quite specifically, judging from the sensor responses according to stepwise antibody immobilization. This was also supported by a negligible sensor response found at bovine serum albumin immobilization. When plotted in double-logarithmic scale for carp vitellogenin concentrations of 0.00675-67.5 nM, a linear relationship was found between analyte concentration and sensor response, together with the limit of detection of 0.00675 nM. The reusability of the immunosensor after the regeneration with 10mM HCl was reasonably good, as presumed from the coefficient of variability of 6.02% for nine repetitive measurements. The model sample prepared by spiking a purified carp vitellogenin into a 10-fold diluted vitellogenin-free carp serum in 9.45 nM showed the response ratio of 96.70% against 9.45 nM of the purified carp vitellogenin. When a female and male carp sera induced with 17beta-estradiol injection were analyzed, biomarker induction was even identifiable at 2000-fold serum dilution.

Clathrin-Mediated Auxin Efflux and Maxima Regulate Hypocotyl Hook Formation and Light-Stimulated Hook Opening in Arabidopsis.

PubMed

Yu, Qinqin; Zhang, Ying; Wang, Juan; Yan, Xu; Wang, Chao; Xu, Jian; Pan, Jianwei

2016-01-04

The establishment of auxin maxima by PIN-FORMED 3 (PIN3)- and AUXIN RESISTANT 1/LIKE AUX1 (LAX) 3 (AUX1/LAX3)-mediated auxin transport is essential for hook formation in Arabidopsis hypocotyls. Until now, however, the underlying regulatory mechanism has remained poorly understood. Here, we show that loss of function of clathrin light chain CLC2 and CLC3 genes enhanced auxin maxima and thereby hook curvature, alleviated the inhibitory effect of auxin overproduction on auxin maxima and hook curvature, and delayed blue light-stimulated auxin maxima reduction and hook opening. Moreover, pharmacological experiments revealed that auxin maxima formation and hook curvature in clc2 clc3 were sensitive to auxin efflux inhibitors 1-naphthylphthalamic acid and 2,3,5-triiodobenzoic acid but not to the auxin influx inhibitor 1-naphthoxyacetic acid. Live-cell imaging analysis further uncovered that loss of CLC2 and CLC3 function impaired PIN3 endocytosis and promoted its lateralization in the cortical cells but did not affect AUX1 localization. Taken together, these results suggest that clathrin regulates auxin maxima and thereby hook formation through modulating PIN3 localization and auxin efflux, providing a novel mechanism that integrates developmental signals and environmental cues to regulate plant skotomorphogenesis and photomorphogenesis. Copyright © 2016 The Author. Published by Elsevier Inc. All rights reserved.

Cooling system for three hook ring segment

DOE Office of Scientific and Technical Information (OSTI.GOV)

Campbell, Christian X.; Eng, Darryl; Lee, Ching-Pang

2014-08-26

A triple hook ring segment including forward, midsection and aft mounting hooks for engagement with respective hangers formed on a ring segment carrier for supporting a ring segment panel, and defining a forward high pressure chamber and an aft low pressure chamber on opposing sides of the midsection mounting hook. An isolation plate is provided on the aft side of the midsection mounting hook to form an isolation chamber between the aft low pressure chamber and the ring segment panel. High pressure air is supplied to the forward chamber and flows to the isolation chamber through crossover passages in themore » midsection hook. The isolation chamber provides convection cooling air to an aft portion of the ring segment panel and enables a reduction of air pressure in the aft low pressure chamber to reduce leakage flow of cooling air from the ring segment.« less

The Topic Is Sandy Hook: A Program for Gifted and Talented Students at Sandy Hook.

ERIC Educational Resources Information Center

Grant, David

"The Topic Is Sandy Hook" is an experiential 10-week program designed to provide special opportunities and educational experiences for 6th to 10th grade gifted and talented students. Sandy Hook, a natural resource in Monmouth County, New Jersey, is unique in its physical and historical features and provides an exceptionally rich environment in…

Antimicrobial resistance and its genetic determinants in aeromonads isolated in ornamental (koi) carp (Cyprinus carpio koi) and common carp (Cyprinus carpio).

PubMed

Cízek, Alois; Dolejská, Monika; Sochorová, Radana; Strachotová, Katerina; Piacková, Veronika; Veselý, Tomás

2010-05-19

The aim of this study was to evaluate antimicrobial susceptibility of Aeromonas spp. isolates from common carp and koi carp coming from randomly chosen farms. The isolates were tested for susceptibility to 8 antimicrobial agents using the standard agar dilution susceptibility test. In all isolates, PCR was used to detect the presence of tet(A-E) genes, integrase genes, and gene cassettes. From the total 72 isolates of motile aeromonads sampled from koi carp, 36 isolates (50%) were resistant to oxytetracycline, 18 (25%) to ciprofloxacin, 5 (7%) to chloramphenicol, 5 (7%) to florfenicol, and 11 (15%) to trimethoprim. Among 49 isolates of motile aeromonads collected from common carp, 20 (41%) were resistant to oxytetracycline, 3 (6%) to chloramphenicol, and 3 (6%) to florfenicol. The resistance of aeromonads isolated from koi carp was significantly higher to ciprofloxacin (P=0.00024). The presence of class 1 integrons was detected in these isolates only (P=0.00024). Tet genes were detected in 40% (48/121) of isolates, with tet(E) being the most dominant. Our results demonstrated a significant difference in the incidence of resistant isolates collected from koi carp and common carp (P=0.00042). This difference can be ascribed to a distinct antibiotic policy established on consumer fish farms versus ornamental fish farms. The potential risk for resistant bacteria to spread and transmit infection to humans should be considered in cases of technological crossover between the two types of fish farms. Copyright 2009 Elsevier B.V. All rights reserved.

String Technique for Anterior Orbital Fish Hook Removal.

PubMed

Starr, Matthew R; Choi, Michael B; Mahr, Michael A; Mettu, Pradeep; Patterson, David F

2018-06-13

Removing fish hooks is a common procedure performed by many emergency department providers. There are several techniques that are commonly employed to aid in successful removal. However, when a fish hook becomes embedded within the orbit, there are limited options as to avoid damaging vital surrounding structures. The authors report the removal of a fish hook within the anterior orbit using the string technique in a 25-year-old patient. The procedure was performed under general anesthesia with the aid of size 5 polyglactin suture wrapped around the hook. The procedure itself took less than 10 seconds and was successful in swiftly and safely removing the hook without damaging surrounding orbital structures. The patient recovered well without any permanent sequelae.

Microanatomy of the cochlear hook

NASA Astrophysics Data System (ADS)

Kwan, Changyow Claire; Tan, Xiaodong; Stock, Stuart R.; Soriano, Carmen; Xiao, Xianghui; Richter, Claus-Peter

2017-09-01

Communication among humans occurs through coding and decoding of acoustic information. The inner ear or cochlea acts as a frequency analyzer and divides the acoustic signal into small frequency bands, which are processed at different sites along the cochlea. The mechano-electrical conversion is accomplished by the soft tissue structures in the cochlea. While the anatomy for most of the cochlea has been well described, a detailed description of the very high frequency and vulnerable cochlear hook region is missing. To study the cochlear hook, mice cochleae were imaged with synchrotron radiation and high-resolution reconstructions have been made from the tomographic scans. This is the first detailed description of the bony and soft tissues of the hook region of the mammalian cochlea.

Maternal inheritance of Nanog ortholog in blunt-snout bream.

PubMed

Yu, Miao; Xue, Ting; Chen, Tiansheng; Fang, Jian; Pan, Qihua; Deng, Yu; Li, Lingyu; Chen, Kai; Wang, Yizhou

2017-12-01

The homeodomain transcription factor Nanog plays an essential role in maintaining pluripotency and self-renewal of embryonic stem cells in mammals. However, the evolutionary conservation of its ortholog in teleosts remains elusive. Here we isolated and characterized a Nanog homolog named as Ma-Nanog in blunt-snout bream (Megalobrama amblycephala). The full-length genomic sequence is 3,326 bp in length and consists of four exons encoding a homeodomain protein of 386 amino acid residues. Comparison of protein sequences revealed that Ma-Nanog is highly homologous to those in other teleosts, particularly in the homeodomain region. During embryogenesis, RNA expression of Nanog was only detected in early developmental embryos, predominantly at the blastula stage, which suggested the transcripts were mainly present in pluripotent stem cells. RNA fluorescence in situ hybridization verified that the signal of the transcripts is present in the germ cells. RNA expression was observed in the oogonia and early stage of oocytes in the ovary, or in the spermatogonia, spermatocytes, and spermatids in the testis. Surprisingly, the transcripts were also detected in adult tissues such as in liver by RT-PCR or qRT-PCR. Subcellular localization of the Nanog protein was also verified in nuclei. Taken together, these results suggested that Ma-Nanog is maternally inherited with conserved features, thus can be potentially used as a marker for stem cells in blunt-snout bream. © 2017 Wiley Periodicals, Inc.

Evaluation of nutritive quality of common carp, Cyprinus carpio L.

NASA Astrophysics Data System (ADS)

Ljubojević, D.; Đorđević, V.; Ćirković, M.

2017-09-01

Common carp is the most important commercial fish species in Serbia. This fish is a valuable source of nutritive components and plays a role in healthy human nutrition. This review evaluates the nutritive quality of common carp including proximate and fatty acid compositions as well as their effects on human health. The fat content and fatty acid composition of carp have been shown to vary due to different environmental factors and particularly due to nutrition. Technology of production and composition of planktonic and benthic organisms in fish ponds have been recognised as significant factors affecting carp meat quality and desirable chemical and fatty acid composition. Carp meat quality but also production parameters and fish health are positively influenced by a balanced feed mixture. Due to the low content of saturated fatty acids and cholesterol plus high levels of unsaturated fatty acids, common carp meat consumption could be linked with reduced risk of different heart diseases in humans. Also, fish proteins can have many beneficial roles in the preservation of human health. This paper emphasises the importance of consumption of common carp in order to prevent many diseases and preserve human health.

Two fault tolerant toggle-hook release

NASA Technical Reports Server (NTRS)

Graves, Thomas Joseph (Inventor); Brown, Christopher William (Inventor)

1991-01-01

A coupling device is disclosed which is mechanically two fault tolerant for release. The device comprises a fastener plate and fastener body, each of which is attachable to a different one of a pair of structures to be joined. The fastener plate and body are coupled by an elongate toggle mounted at one end in a socket on the fastener plate for universal pivotal movement thereon. The other end of the toggle is received in an opening in the fastener body and adapted for limited pivotal movement therein. The toggle is adapted to be restrained by three latch hooks arranged in symmetrical equiangular spacing about the axis of the toggle, each hook being mounted on the fastener body for pivotal movement between an unlatching non-contact position with respect to the toggle and a latching position in engagement with a latching surface of the toggle. The device includes releasable lock means for locking each latch hook in its latching position whereby the toggle couples the fastener plate to the fastener body and means for releasing the lock means to unlock each said latch hook from the latch position whereby the unlocking of at least one of the latch hooks from its latching position results in the decoupling of the fastener plate from the fastener body.

30 CFR 56.19075 - Use of open hooks.

Code of Federal Regulations, 2010 CFR

2010-07-01

... 30 Mineral Resources 1 2010-07-01 2010-07-01 false Use of open hooks. 56.19075 Section 56.19075 Mineral Resources MINE SAFETY AND HEALTH ADMINISTRATION, DEPARTMENT OF LABOR METAL AND NONMETAL MINE... Hoisting Procedures § 56.19075 Use of open hooks. Open hooks shall not be used to hoist buckets or other...

30 CFR 56.19075 - Use of open hooks.

Code of Federal Regulations, 2013 CFR

2013-07-01

... 30 Mineral Resources 1 2013-07-01 2013-07-01 false Use of open hooks. 56.19075 Section 56.19075 Mineral Resources MINE SAFETY AND HEALTH ADMINISTRATION, DEPARTMENT OF LABOR METAL AND NONMETAL MINE... Hoisting Procedures § 56.19075 Use of open hooks. Open hooks shall not be used to hoist buckets or other...

30 CFR 56.19075 - Use of open hooks.

Code of Federal Regulations, 2014 CFR

2014-07-01

... 30 Mineral Resources 1 2014-07-01 2014-07-01 false Use of open hooks. 56.19075 Section 56.19075 Mineral Resources MINE SAFETY AND HEALTH ADMINISTRATION, DEPARTMENT OF LABOR METAL AND NONMETAL MINE... Hoisting Procedures § 56.19075 Use of open hooks. Open hooks shall not be used to hoist buckets or other...

30 CFR 57.19075 - Use of open hooks.

Code of Federal Regulations, 2010 CFR

2010-07-01

... 30 Mineral Resources 1 2010-07-01 2010-07-01 false Use of open hooks. 57.19075 Section 57.19075 Mineral Resources MINE SAFETY AND HEALTH ADMINISTRATION, DEPARTMENT OF LABOR METAL AND NONMETAL MINE... Hoisting Procedures § 57.19075 Use of open hooks. Open hooks shall not be used to hoist buckets or other...

30 CFR 56.19075 - Use of open hooks.

Code of Federal Regulations, 2012 CFR

2012-07-01

... 30 Mineral Resources 1 2012-07-01 2012-07-01 false Use of open hooks. 56.19075 Section 56.19075 Mineral Resources MINE SAFETY AND HEALTH ADMINISTRATION, DEPARTMENT OF LABOR METAL AND NONMETAL MINE... Hoisting Procedures § 56.19075 Use of open hooks. Open hooks shall not be used to hoist buckets or other...

30 CFR 57.19075 - Use of open hooks.

Code of Federal Regulations, 2013 CFR

2013-07-01

... 30 Mineral Resources 1 2013-07-01 2013-07-01 false Use of open hooks. 57.19075 Section 57.19075 Mineral Resources MINE SAFETY AND HEALTH ADMINISTRATION, DEPARTMENT OF LABOR METAL AND NONMETAL MINE... Hoisting Procedures § 57.19075 Use of open hooks. Open hooks shall not be used to hoist buckets or other...

30 CFR 57.19075 - Use of open hooks.

Code of Federal Regulations, 2011 CFR

2011-07-01

... 30 Mineral Resources 1 2011-07-01 2011-07-01 false Use of open hooks. 57.19075 Section 57.19075 Mineral Resources MINE SAFETY AND HEALTH ADMINISTRATION, DEPARTMENT OF LABOR METAL AND NONMETAL MINE... Hoisting Procedures § 57.19075 Use of open hooks. Open hooks shall not be used to hoist buckets or other...

30 CFR 57.19075 - Use of open hooks.

Code of Federal Regulations, 2014 CFR

2014-07-01

... 30 Mineral Resources 1 2014-07-01 2014-07-01 false Use of open hooks. 57.19075 Section 57.19075 Mineral Resources MINE SAFETY AND HEALTH ADMINISTRATION, DEPARTMENT OF LABOR METAL AND NONMETAL MINE... Hoisting Procedures § 57.19075 Use of open hooks. Open hooks shall not be used to hoist buckets or other...

30 CFR 56.19075 - Use of open hooks.

Code of Federal Regulations, 2011 CFR

2011-07-01

... 30 Mineral Resources 1 2011-07-01 2011-07-01 false Use of open hooks. 56.19075 Section 56.19075 Mineral Resources MINE SAFETY AND HEALTH ADMINISTRATION, DEPARTMENT OF LABOR METAL AND NONMETAL MINE... Hoisting Procedures § 56.19075 Use of open hooks. Open hooks shall not be used to hoist buckets or other...

30 CFR 57.19075 - Use of open hooks.

Code of Federal Regulations, 2012 CFR

2012-07-01

... 30 Mineral Resources 1 2012-07-01 2012-07-01 false Use of open hooks. 57.19075 Section 57.19075 Mineral Resources MINE SAFETY AND HEALTH ADMINISTRATION, DEPARTMENT OF LABOR METAL AND NONMETAL MINE... Hoisting Procedures § 57.19075 Use of open hooks. Open hooks shall not be used to hoist buckets or other...

Drawings of fossils by Robert Hooke and Richard Waller

PubMed Central

Kusukawa, Sachiko

2013-01-01

The drawings of fossils by Robert Hooke and Richard Waller that were the basis of the engravings in Hooke's Posthumous works (1705) are published here for the first time. The drawings show that both Hooke and Waller were proficient draftsmen with a keen eye for the details of petrified objects. These drawings provided Hooke with a polemic edge in making the case for the organic origins of ‘figured stones’.

Impact of moderate silver carp biomass gradient on zooplankton communities in a eutrophic reservoir. Consequences for the use of silver carp in biomanipulation.

PubMed

Domaizon, I; Dévaux, J

1999-07-01

We examined the impacts of moderate gradient silver carp biomass (five levels from 0 to 36 g.m-3, i.e. about 0-792 kg.ha-1) on zooplankton communities of the eutrophic Villerest reservoir (France). During our mesocosm experiment changes in zooplankton assemblages were dependent on silver carp biomass. In the fishless and low fish biomass treatments, zooplankton abundance increased through time, owing to a peak in cladoceran density, but decreased (mainly cladocerans) at highest fish biomass. Copepods and rotifers were less affected at the highest fish biomass and dominated zooplankton communities. We highlighted that the presence of high silver carp biomass could lead to changes in phytoplankton assemblage via the impact on herbivorous zooplankton. Since silver carp efficiently graze on particles > 20 microns, the suppression of herbivorous cladocerans could result in an increase in small size algae (< 20 microns) abundance since these species would be released from grazers as well as competitors (large algae grazed by silver carp) and nutrients levels would be enhanced by fish internal loading. Our results showed that the use of low silver carp biomass (< 200 kg.ha-1) would allow us to minimize these negative effects.

Sex-specific markers developed by next-generation sequencing confirmed an XX/XY sex determination system in bighead carp (Hypophthalmichehys nobilis) and silver carp (Hypophthalmichthys molitrix).

PubMed

Liu, Haiyang; Pang, Meixia; Yu, Xiaomu; Zhou, Ying; Tong, Jingou; Fu, Beide

2018-01-05

Sex-specific markers are powerful tools for identifying sex-determination system in various animals. Bighead carp (Hypophthalmichehys nobilis) and silver carp (Hypophthalmichthys molitrix) are two of the most important edible fish in Asia, which have a long juvenility period that can lasts for 4-5 years. In this study, we found one sex-specific marker by next-generation sequencing together with bioinformatics analysis in bighead carp. The male-specific markers were used to perform molecular sexing in the progenies of artificial gynogenetic diploids and found all progenies (n = 160) were females. Meanwhile, around 1 : 1 sex ratio was observed in a total of 579 juvenile offspring from three other families. To further extend the male-specific region, we performed genome walking and got a male-specific sequence of 8,661 bp. Five pairs of primers were designed and could be used to efficiently distinguish males from females in bighead carp and silver carp. The development of these male-specific markers and results of their molecular sexing in different populations provide strong evidence for a sex determination system of female homogametry or male heterogametry (XX/XY) in bighead carp and silver carp. To the best of our knowledge, this is the first report of effective sex-specific markers in these two large carp species. © The Author(s) 2018. Published by Oxford University Press on behalf of Kazusa DNA Research Institute.

Inferences of Diplodocoid (Sauropoda: Dinosauria) Feeding Behavior from Snout Shape and Microwear Analyses

PubMed Central

Whitlock, John A.

2011-01-01

Background As gigantic herbivores, sauropod dinosaurs were among the most important members of Mesozoic communities. Understanding their ecology is fundamental to developing a complete picture of Jurassic and Cretaceous food webs. One group of sauropods in particular, Diplodocoidea, has long been a source of debate with regard to what and how they ate. Because of their long lineage duration (Late Jurassic-Late Cretaceous) and cosmopolitan distribution, diplodocoids formed important parts of multiple ecosystems. Additionally, fortuitous preservation of a large proportion of cranial elements makes them an ideal clade in which to examine feeding behavior. Methodology/Principal Findings Hypotheses of various browsing behaviors (selective and nonselective browsing at ground-height, mid-height, or in the upper canopy) were examined using snout shape (square vs. round) and dental microwear. The square snouts, large proportion of pits, and fine subparallel scratches in Apatosaurus, Diplodocus, Nigersaurus, and Rebbachisaurus suggest ground-height nonselective browsing; the narrow snouts of Dicraeosaurus, Suuwassea, and Tornieria and the coarse scratches and gouges on the teeth of Dicraeosaurus suggest mid-height selective browsing in those taxa. Comparison with outgroups (Camarasaurus and Brachiosaurus) reinforces the inferences of ground- and mid-height browsing and the existence of both non-selective and selective browsing behaviors in diplodocoids. Conclusions/Significance These results reaffirm previous work suggesting the presence of diverse feeding strategies in sauropods and provide solid evidence for two different feeding behaviors in Diplodocoidea. These feeding behaviors can subsequently be tied to paleoecology, such that non-selective, ground-height behaviors are restricted to open, savanna-type environments. Selective browsing behaviors are known from multiple sauropod clades and were practiced in multiple environments. PMID:21494685

Hook1 inhibits malignancy and epithelial-mesenchymal transition in hepatocellular carcinoma.

PubMed

Sun, Xu; Zhang, Qi; Chen, Wei; Hu, Qida; Lou, Yu; Fu, Qi-Han; Zhang, Jing-Ying; Chen, Yi-Wen; Ye, Long-Yun; Wang, Yi; Xie, Shang-Zhi; Hu, Li-Qiang; Liang, Ting-Bo; Bai, Xue-Li

2017-07-01

Hook1 is a member of the hook family of coiled-coil proteins, which is recently found to be associated with malignant tumors. However, its biological function in hepatocellular carcinoma is yet unknown. Here, we evaluated the Hook1 levels in human hepatocellular carcinoma samples and matched peritumoral tissues by real-time polymerase chain reaction. Small interfering RNA knockdown and a transforming growth factor-β-induced epithelial-mesenchymal transition model were employed to investigate the biological effects of Hook1 in hepatocellular carcinoma. Our results indicated that Hook1 levels were significantly lower in hepatocellular carcinoma tissues than in the peritumoral tissues. In addition, Hook1 expression was significantly associated with hepatocellular carcinoma malignancy. Hook1 was downregulated after transforming growth factor-β-induced epithelial-mesenchymal transition. Moreover, Hook1 knockdown promoted epithelial-mesenchymal transition and attenuated the sensitivity of hepatocellular carcinoma cells to doxorubicin. In summary, our results indicate that downregulation of Hook1 plays a pivotal role in hepatocellular carcinoma progression via epithelial-mesenchymal transition. Hook1 may be used as a novel marker and therapeutic molecular target in hepatocellular carcinoma.

BOOK REVIEW: Robert Hooke and the Royal Society

NASA Astrophysics Data System (ADS)

Brown, Neil

2000-01-01

Many physics students only come across Hooke when they learn his law of stretching springs, which is a pity because it is just one of his contributions to progress in science, and a minor one at that. His, Micrographia, the first great book of microscopical observations, arouses admiration to this day. He was also active in horology, astronomy, geology and surveying, and he took part in biological experiments, transfusing blood between animals. Much of his work was done while he was curator of experiments for the Royal Society, in which he was involved almost from its foundation. This was by no means a full-time occupation, however. After the Great Fire of London, Hooke was appointed one of the three surveyors for the rebuilding of the city. One of the others was Christopher Wren, a lifelong friend. In this role Hooke was responsible for the design of several buildings, including the Monument. Nichols writes about all these activities, as well as Hooke's childhood, his education at Westminster School, the University of Oxford when Hooke was an undergraduate, and the founding of the Royal Society. The book draws on research for a master's degree. Turning a dissertation into a popular book is risky. The author has avoided the pitfall of making it too academic, but the result is not satisfying. Nichols seems overawed by Hooke and his work, frequently seeming to credit Hooke with a far-reaching influence that he did not necessarily have. There may be a case for lauding Hooke as the father of English microscopy, the father of English meteorology, and the founder of English geology and earth sciences, but it needs to be made much more critically, even in a popular work. Hooke was full of good ideas, but he rarely continued long enough to put them into practice. There is no doubt that Hooke proposed using a balance wheel and spring to improve the timekeeping of a watch, for example, but he did not have a watch made to his design until after Christiaan Huygens had

Environment shapes the fecal microbiome of invasive carp species.

PubMed

Eichmiller, Jessica J; Hamilton, Matthew J; Staley, Christopher; Sadowsky, Michael J; Sorensen, Peter W

2016-08-12

Although the common, silver, and bighead carps are native and sparsely distributed in Eurasia, these fish have become abundant and invasive in North America. An understanding of the biology of these species may provide insights into sustainable control methods. The animal-associated microbiome plays an important role in host health. Characterization of the carp microbiome and the factors that affect its composition is an important step toward understanding the biology and interrelationships between these species and their environments. We compared the fecal microbiomes of common, silver, and bighead carps from wild and laboratory environments using Illumina sequencing of bacterial 16S ribosomal RNA (rRNA). The fecal bacterial communities of fish were diverse, with Shannon indices ranging from 2.3 to 4.5. The phyla Proteobacteria, Firmicutes, and Fusobacteria dominated carp guts, comprising 76.7 % of total reads. Environment played a large role in shaping fecal microbial community composition, and microbiomes among captive fishes were more similar than among wild fishes. Although differences among wild fishes could be attributed to feeding preferences, diet did not strongly affect microbial community structure in laboratory-housed fishes. Comparison of wild- and lab-invasive carps revealed five shared OTUs that comprised approximately 40 % of the core fecal microbiome. The environment is a dominant factor shaping the fecal bacterial communities of invasive carps. Captivity alters the microbiome community structure relative to wild fish, while species differences are pronounced within habitats. Despite the absence of a true stomach, invasive carp species exhibited a core microbiota that warrants future study.

Fish-hook injuries: a risk for fishermen

PubMed Central

2010-01-01

Fishing is one of the best known and practiced human activities. However, you should remember that, when casting the hook from the riverbank or grasping it to add bait, fishermen run a real risk of injury if the hook punctures the skin. Briefly we describe a case where a young, 32-year-old fisherman who was reeling the hook back to shore when it hit him in the face and embedded itself in his upper eyelid. Upon examination, the eye was found to be unharmed and the hook was removed through a small incision and the aid of a local anesthetic. In the light of this case report, we think it a good idea to advise our friends and patients who we know to be fishermen to wear some form of eye protection as a precaution. PMID:21156039

Black Carp: Biological synopsis and risk assessment of an introduced fish

USGS Publications Warehouse

Nico, L.G.; Williams, J.D.; Jelks, H.L.

2005-01-01

Major subjects addressed are (1) taxonomy, description, and distinguishing characteristics of the species; (2) native distribution; (3) biology and natural history, with emphasis on diet and reproduction; (4) history of the species in world aquaculture; (5) history of introduction within and outside the United States; (6) use as a biological control control agent, including a review of digenetic trematodes and snail-borne parasites of special concern and methods used for control; (7) alternatives to the use of black carp; (8) environmental tolerance and potential geographic range; and (9) risks associated with its introduction. The book also includes substantial information on the other Chinese carp species, including bighead carp, silver carp, and grass carp.

Comparative study on the gut microbiotas of four economically important Asian carp species.

PubMed

Li, Xinghao; Yu, Yuhe; Li, Chang; Yan, Qingyun

2018-05-07

Gut microbiota of four economically important Asian carp species (silver carp, Hypophthalmichthys molitrix; bighead carp, Hypophthalmichthys nobilis; grass carp, Ctenopharyngodon idella; common carp, Cyprinus carpio) were compared using 16S rRNA gene pyrosequencing. Analysis of more than 590,000 quality-filtered sequences obtained from the foregut, midgut and hindgut of these four carp species revealed high microbial diversity among the samples. The foregut samples of grass carp exhibited more than 1,600 operational taxonomy units (OTUs) and the highest alpha-diversity index, followed by the silver carp foregut and midgut. Proteobacteria, Firmicutes, Bacteroidetes and Fusobacteria were the predominant phyla regardless of fish species or gut type. Pairwise (weighted) UniFrac distance-based permutational multivariate analysis of variance with fish species as a factor produced significant association (P<0.01). The gut microbiotas of all four carp species harbored saccharolytic or proteolytic microbes, likely in response to the differences in their feeding habits. In addition, extensive variations were also observed even within the same fish species. Our results indicate that the gut microbiotas of Asian carp depend on the exact species, even when the different species were cohabiting in the same environment. This study provides some new insights into developing commercial fish feeds and improving existing aquaculture strategies.

USGS science and technology help managers battle invading Asian carp

USGS Publications Warehouse

Kolar, Cynthia S.; Morrison, Sandra S.

2016-09-28

The U.S. Geological Survey (USGS) conducts Asian carp research focused on early detection, risk assessment, and development of control tools and strategies. The goals are to prevent the establishment of invasive Asian carp in the Great Lakes and to reduce their impacts in the Ohio River and Mississippi River Basins and elsewhere. Managers can use the information, tools, and strategies for early detection of Asian carp and to control them when their presence is first evident. New detection and control tools are designed to accommodate expansion to other invasive species and application in geographically diverse areas.This USGS focus complements goals of the Great Lakes Restoration Initiative (GLRI), a multi-agency collaboration started in 2010 to protect and restore the Great Lakes. As a member of the Asian Carp Regional Coordinating Committee, which guides Asian carp efforts, the USGS works closely with Federal and State agencies, Canada, and others to address high-priority Asian carp issues and provide science to inform management decisions.The USGS has gained extensive knowledge of Asian carp biology and life history over the past 30 years. That knowledge guides the design, development, and application of control strategies, and is essential for developing approaches in line with modern principles and practices of integrated pest management (IPM). IPM is a process used to solve pest problems while minimizing risks to people and the environment.

Comparative Transcriptome Analysis Reveals the Genetic Basis of Skin Color Variation in Common Carp

PubMed Central

Jiang, Yanliang; Zhang, Songhao; Xu, Jian; Feng, Jianxin; Mahboob, Shahid; Al-Ghanim, Khalid A.; Sun, Xiaowen; Xu, Peng

2014-01-01

Background The common carp is an important aquaculture species that is widely distributed across the world. During the long history of carp domestication, numerous carp strains with diverse skin colors have been established. Skin color is used as a visual criterion to determine the market value of carp. However, the genetic basis of common carp skin color has not been extensively studied. Methodology/Principal Findings In this study, we performed Illumina sequencing on two common carp strains: the reddish Xingguo red carp and the brownish-black Yellow River carp. A total of 435,348,868 reads were generated, resulting in 198,781 assembled contigs that were used as reference sequences. Comparisons of skin transcriptome files revealed 2,012 unigenes with significantly different expression in the two common carp strains, including 874 genes that were up-regulated in Xingguo red carp and 1,138 genes that were up-regulated in Yellow River carp. The expression patterns of 20 randomly selected differentially expressed genes were validated using quantitative RT-PCR. Gene pathway analysis of the differentially expressed genes indicated that melanin biosynthesis, along with the Wnt and MAPK signaling pathways, is highly likely to affect the skin pigmentation process. Several key genes involved in the skin pigmentation process, including TYRP1, SILV, ASIP and xCT, showed significant differences in their expression patterns between the two strains. Conclusions In this study, we conducted a comparative transcriptome analysis of Xingguo red carp and Yellow River carp skins, and we detected key genes involved in the common carp skin pigmentation process. We propose that common carp skin pigmentation depends upon at least three pathways. Understanding fish skin color genetics will facilitate future molecular selection of the fish skin colors with high market values. PMID:25255374

Comparative transcriptome analysis reveals the genetic basis of skin color variation in common carp.

PubMed

Jiang, Yanliang; Zhang, Songhao; Xu, Jian; Feng, Jianxin; Mahboob, Shahid; Al-Ghanim, Khalid A; Sun, Xiaowen; Xu, Peng

2014-01-01

The common carp is an important aquaculture species that is widely distributed across the world. During the long history of carp domestication, numerous carp strains with diverse skin colors have been established. Skin color is used as a visual criterion to determine the market value of carp. However, the genetic basis of common carp skin color has not been extensively studied. In this study, we performed Illumina sequencing on two common carp strains: the reddish Xingguo red carp and the brownish-black Yellow River carp. A total of 435,348,868 reads were generated, resulting in 198,781 assembled contigs that were used as reference sequences. Comparisons of skin transcriptome files revealed 2,012 unigenes with significantly different expression in the two common carp strains, including 874 genes that were up-regulated in Xingguo red carp and 1,138 genes that were up-regulated in Yellow River carp. The expression patterns of 20 randomly selected differentially expressed genes were validated using quantitative RT-PCR. Gene pathway analysis of the differentially expressed genes indicated that melanin biosynthesis, along with the Wnt and MAPK signaling pathways, is highly likely to affect the skin pigmentation process. Several key genes involved in the skin pigmentation process, including TYRP1, SILV, ASIP and xCT, showed significant differences in their expression patterns between the two strains. In this study, we conducted a comparative transcriptome analysis of Xingguo red carp and Yellow River carp skins, and we detected key genes involved in the common carp skin pigmentation process. We propose that common carp skin pigmentation depends upon at least three pathways. Understanding fish skin color genetics will facilitate future molecular selection of the fish skin colors with high market values.

Robert Hooke's model of memory.

PubMed

Hintzman, Douglas L

2003-03-01

In 1682 the scientist and inventor Robert Hooke read a lecture to the Royal Society of London, in which he described a mechanistic model of human memory. Yet few psychologists today seem to have heard of Hooke's memory model. The lecture addressed questions of encoding, memory capacity, repetition, retrieval, and forgetting--some of these in a surprisingly modern way. Hooke's model shares several characteristics with the theory of Richard Semon, which came more than 200 years later, but it is more complete. Among the model's interesting properties are that (1) it allows for attention and other top-down influences on encoding; (2) it uses resonance to implement parallel, cue-dependent retrieval; (3) it explains memory for recency; (4) it offers a single-system account of repetition priming; and (5) the power law of forgetting can be derived from the model's assumptions in a straightforward way.

First direct confirmation of grass carp spawning in a Great Lakes tributary

USGS Publications Warehouse

Embke, Holly S.; Kocovsky, Patrick M.; Richter, Catherine A.; Pritt, Jeremy J.; Christine M. Mayer,; Qian, Song

2016-01-01

Grass carp (Ctenopharyngodon idella), an invasive species of Asian carp, has been stocked for many decades in the United States for vegetation control. Adult individuals have been found in all of the Great Lakes except Lake Superior, but no self-sustaining populations have yet been identified in Great Lakes tributaries. In 2012, a commercial fisherman caught four juvenile diploid grass carp in the Sandusky River, a major tributary to Lake Erie. Otolith microchemistry and the capture location of these fish permitted the conclusion that they were most likely produced in the Sandusky River. Due to this finding, we sampled ichthyoplankton using paired bongo net tows and larval light traps during June–August of 2014 and 2015 to determine if grass carp are spawning in the Sandusky River. From the samples collected in 2015, we identified and staged eight eggs that were morphologically consistent with grass carp. Five eggs were confirmed as grass carp using quantitative Polymerase Chain Reaction for a grass carp-specific marker, while the remaining three were retained for future analysis. Our finding confirms that grass carp are naturally spawning in this Great Lakes tributary. All eggs were collected during high-flow events, either on the day of peak flow or 1–2 days following peak flow, supporting an earlier suggestion that high flow conditions favor grass carp spawning. The next principal goal is to identify the spawning and hatch location(s) for the Sandusky River. Predicting locations and conditions where grass carp spawning is most probable may aid targeted management efforts.

Hooking Up and Identity Development of Female College Students

ERIC Educational Resources Information Center

Kooyman, Leslie; Pierce, Gloria; Zavadil, Amy

2011-01-01

Hooking up generally involves casual sex with noncommittal partners. Hooking up is prevalent on college campuses today and can negatively affect the identity development of female students. The authors examined this phenomenon with a feminist developmental perspective, evaluating hooking up in the context of sexual risk taking with physical and…

Genetic diversity and variation of mitochondrial DNA in native and introduced bighead carp

USGS Publications Warehouse

Li, Si-Fa; Yang, Qin-Ling; Xu, Jia-Wei; Wang, Cheng-Hui; Chapman, Duane C.; Lu, Guoping

2010-01-01

The bighead carp Hypophthalmichthys nobilis is native to China but has been introduced to over 70 countries and is established in many large river systems. Genetic diversity and variation in introduced bighead carp have not previously been evaluated, and a systematic comparison among fish from different river systems was unavailable. In this study, 190 bighead carp specimens were sampled from five river systems in three countries (Yangtze, Pearl, and Amur rivers, China; Danube River, Hungary; Mississippi River basin, USA) and their mitochondrial 16S ribosomal RNA gene and D-loop region were sequenced (around 1,345 base pairs). Moderate genetic diversity was found in bighead carp, ranging from 0.0014 to 0.0043 for nucleotide diversity and from 0.6879 to 0.9333 for haplotype diversity. Haplotype analysis provided evidence that (1) multiple haplotype groups might be present among bighead carp, (2) bighead carp probably originated from the Yangtze River, and (3) bighead carp in the Mississippi River basin may have some genetic ancestry in the Danube River. The analysis of molecular variance showed significant genetic differentiation among these five populations but also revealed limited differentiation between the Yangtze and Amur River bighead carp. This large-scale study of bighead carp genetic diversity and variation provides the first global perspective of bighead carp in the context of biodiversity conservation as well as invasive species control and management.

Seasonal trends in eDNA detection and occupancy of bigheaded carps

USGS Publications Warehouse

Erickson, Richard A.; Merkes, Christopher; Jackson, Craig; Goforth, Reuben; Amberg, Jon J.

2017-01-01

Bigheaded carps, which include silver and bighead carp, are threatening to invade the Great Lakes. These species vary seasonally in distribution and abundance due to environmental conditions such as precipitation and temperature. Monitoring this seasonal movement is important for management to control the population size and spread of the species. We examined if environmental DNA (eDNA) approaches could detect seasonal changes of these species. To do this, we developed a novel genetic marker that was able to both detect and differentiate bighead and silver carp DNA. We used the marker, combined with a novel occupancy model, to study the occurrence of bigheaded carps at 3 sites on the Wabash River over the course of a year. We studied the Wabash River because of concerns that carps may be able to use the system to invade the Great Lakes via a now closed (ca. 2017) connection at Eagle Marsh between the Wabash River's watershed and the Great Lakes' watershed. We found seasonal trends in the probability of detection and occupancy that varied across sites. These findings demonstrate that eDNA methods can detect seasonal changes in bigheaded carps densities and suggest that the amount of eDNA present changes seasonally. The site that was farthest upstream and had the lowest carp densities exhibited the strongest seasonal trends for both detection probabilities and sample occupancy probabilities. Furthermore, other observations suggest that carps seasonally leave this site, and we were able to detect this with our eDNA approach.

Estimating freshwater turtle mortality rates and population declines following hook ingestion.

PubMed

Steen, David A; Robinson, Orin J

2017-12-01

Freshwater turtle populations are susceptible to declines following small increases in the mortality of adults, making it essential to identify and understand potential threats. Freshwater turtles ingest fish hooks associated with recreational angling, and this is likely a problem because hook ingestion is a source of additive mortality for sea turtles. We used a Bayesian-modeling framework, observed rates of hook ingestion by freshwater turtles, and mortality of sea turtles from hook ingestion to examine the probability that a freshwater turtle in a given population ingests a hook and subsequently dies from it. We used the results of these analyses and previously published life-history data to simulate the effects of hook ingestion on population growth for 3 species of freshwater turtle. In our simulation, the probability that an individual turtle ingests a hook and dies as a result was 1.2-11%. Our simulation results suggest that this rate of mortality from hook ingestion is sufficient to cause population declines. We believe we have identified fish-hook ingestion as a serious yet generally overlooked threat to the viability of freshwater turtle populations. © 2017 Society for Conservation Biology.

Complete mitochondrial genome of Xingguo red carp (Cyprinus carpio var. singuonensis) and purse red carp (Cyprinus carpio var. wuyuanensis).

PubMed

Hu, Guang-Fu; Liu, Xiang-Jiang; Li, Zhong; Liang, Hong-Wei; Hu, Shao-Na; Zou, Gui-Wei

2016-01-01

The complete mitochondrial genomes of Xingguo red carp (Cyprinus carpio var. singuonensis) and purse red carp (Cyprinus carpio var. wuyuanensis) were sequenced. Comparison of these two mitochondrial genomes revealed that the mtDNAs of these two common carp varieties were remarkably similar in genome length, gene order and content, and AT content. However, size variation between these two mitochondrial genomes presented here showed 39 site differences in overall length. About 2 site differences were located in rRNAs, 3 in tRNAs, 3 in the control region, 31 in protein-coding genes. Thirty-one variable bases in the protein-coding regions between the two varieties mitochondrial sequences led to three variable amino acids, which were mainly located in the protein ND5 and ND4.

Predictive Management of Asian Carps in the Upper Mississippi River System

USGS Publications Warehouse

Vondracek, Bruce C.; Carlson, Andrew K.

2014-01-01

Prolific non-native organisms pose serious threats to ecosystems and economies worldwide. Nonnative bighead carp (Hypophthalmichthys nobilis) and silver carp (H. molitrix), collectively referred to as Asian carps, continue to colonize aquatic ecosystems throughout the central United States. These species are r-selected, exhibiting iteroparous spawning, rapid growth, broad environmental tolerance, high density, and long-distance movement. Hydrological, thermal, and physicochemical conditions are favorable for establishment beyond the current range, rendering containment and control imperative. Ecological approaches to confine Asian carp populations and prevent colonization characterize contemporary management in the United States. Foraging and reproduction of Asian carps govern habitat selection and movement, providing valuable insight for predictive control. Current management approaches are progressive and often anticipatory but deficient in human dimensions. We define predictive management of Asian carps as synthesis of ecology and human dimensions at regional and local scales to develop strategies for containment and control. We illustrate predictive management in the Upper Mississippi River System and suggest resource managers integrate predictive models, containment paradigms, and human dimensions to design effective, socially acceptable management strategies. Through continued research, university-agency collaboration, and public engagement, predictive management of Asian carps is an auspicious paradigm for preventing and alleviating consequences of colonization in the United States.

Cut-it-out technique for ocular fish-hook injury.

PubMed

Ahmad, Syed Shoeb; Seng, Chiang Wai; Ghani, Shuaibah Abdul; Lee, Jane F

2013-10-01

An 11-year-old boy was involved in an injury with a fish-hook to his eye. The hook had impaled itself to the cornea and deeper structures. There was associated corneal edema and hyphema, making visualization difficult. In this case, we performed the unusual "cut-it-out" technique to remove the hook from the eye.

In vitro Antioxidant of a Water-Soluble Polysaccharide from Dendrobium fimhriatum Hook.var.oculatum Hook

PubMed Central

Luo, Aoxue; Fan, Yijun

2011-01-01

A water-soluble crude polysaccharide (DFHP) obtained from the aqueous extracts of the stem of Dendrobium fimhriatum Hook.var.oculatum Hook through hot water extraction followed by ethanol precipitation, was found to have an average molecular weight (Mw) of about 209.3 kDa. Monosaccharide analysis revealed that DFHP was composed of mannose, glucose and galactose in a content ratio of 37.52%; 43.16%; 19.32%. The investigation of antioxidant activity in vitro showed that DFHP is a potential antioxidant. PMID:21747725

Asian carp behavior in response to static water gun firing

USGS Publications Warehouse

Layhee, Megan J.; Gross, Jackson A.; Parsley, Michael J.; Romine, Jason G.; Glover, David C.; Suski, Cory D.; Wagner, Tristany L.; Sepulveda, Adam J.; Gresswell, Robert E.

2013-01-01

The potential for invasion of Asian carp into the Great Lakes has ecological and socio-economic implications. If they become established, Asian carp are predicted to alter lake ecosystems and impact commercial and recreational fisheries. The Chicago Sanitary and Shipping Canal is an important biological conduit between the Mississippi River Basin, where invasive Asian carp are abundant, and the Great Lakes. Millions of dollars have been spent to erect an electric barrier defense in the canal to prevent movement of Asian carp into the Great Lakes, but the need for additional fish deterrent technologies to supplement the existing barrier is warranted. Scientists with the U.S. Geological Survey Northern Rocky Mountain Science Center are examining seismic water gun technology, formerly used in oceanic oil exploration, as a fish deterrent. The goal of the current study is to employ telemetry and sonar monitoring equipment to assess the behavioral response of Asian carp to seismic water guns and the sound energy it generates.

Can hook-bending be let off the hook? Bending/unbending of pliant tools by cockatoos.

PubMed

Laumer, I B; Bugnyar, T; Reber, S A; Auersperg, A M I

2017-09-13

The spontaneous crafting of hook-tools from bendable material to lift a basket out of a vertical tube in corvids has widely been used as one of the prime examples of animal tool innovation. However, it was recently suggested that the animals' solution was hardly innovative but strongly influenced by predispositions from habitual tool use and nest building. We tested Goffin's cockatoo, which is neither a specialized tool user nor a nest builder, on a similar task set-up. Three birds individually learned to bend hook tools from straight wire to retrieve food from vertical tubes and four subjects unbent wire to retrieve food from horizontal tubes. Pre-experience with ready-made hooks had some effect but was not necessary for success. Our results indicate that the ability to represent and manufacture tools according to a current need does not require genetically hardwired behavioural routines, but can indeed arise innovatively from domain general cognitive processing. © 2017 The Authors.

Life History Attributes of Asian Carps in the Upper Mississippi River System

DTIC Science & Technology

2007-05-01

Mature ovary of a female silver carp. Each small white spherical structure is a mature egg 4 ERDC/TN ANSRP-07-1 May 2007 Silver carp in the...weight/fish weight), and fecundity (number of eggs per female ) of each silver and bighead carp were quantified. Diets of silver carp from the MMR also...through November, with gonads ranging from 1 to 13 percent of body weight. Females with mature ovaries (Figure 3) were present as early as age 2 years

Apparent digestibility of Asian carp and common carp-derived fish meals in feeds for hybrid striped bass Morone saxatilis female x M. chrysops male and rainbow trout Oncorhynchus mykiss

USDA-ARS?s Scientific Manuscript database

Apparent digestibility coefficients (ADCs) of nutrients (crude protein, amino acids, crude lipid, fatty acids, and minerals) were determined for fish meals derived from menhaden, Asian carp (combination of silver and bighead carps), and common carp in feeds for hybrid striped bass and rainbow trout....

Ethylene Is Not Responsible for Phytochrome-Mediated Apical Hook Exaggeration in Tomato

PubMed Central

Takahashi-Asami, Miki; Shichijo, Chizuko; Tsurumi, Seiji; Hashimoto, Tohru

2016-01-01

The apical hook of tomato seedlings is exaggerated by phytochrome actions, while in other species such as bean, pea and Arabidopsis, the hook is exaggerated by ethylene and opens by phytochrome actions. The present study was aimed to clarify mainly whether ethylene is responsible for the phytochrome-mediated hook exaggeration of tomato seedlings. Dark-grown 5-day-old seedlings were subjected to various ways of ethylene application in the dark as well as under the actions of red (R) or far-red light (FR). The ethylene emitted by seedlings was also quantified relative to hook exaggeration. The results show: Ambient ethylene, up-to about 1.0 μL L-1, suppressed (opened) the hooks formed in the dark as well as the ones exaggerated by R or FR, while at 3.0–10 μL L-1 it enhanced (closed) the hook only slightly as compared with the most-suppressed level at about 1.0 μL L-1. Treatment with 1-aminocyclopropane-1-carboxylic acid (ACC), the immediate precursor of ethylene biosynthesis, did not enhance the hook, only mimicking the suppressive effects of ambient ethylene. The biosynthesis inhibitor, CoCl2 or aminoethoxyvinylglycine, enhanced hook curvature, and the enhancement was canceled by supplement of ethylene below 1.0 μL L-1. Auxin transport inhibitor, N-1-naphthylphthalamic acid, by contrast, suppressed curvature markedly without altering ethylene emission. The effects of the above-stated treatments did not differentiate qualitatively among the R-, FR-irradiated seedlings and dark control so as to explain phytochrome-mediated hook exaggeration. In addition, ethylene emission by seedlings was affected neither by R nor FR at such fluences as to cause hook exaggeration. In conclusion, (1) ethylene suppresses not only the light-exaggerated hook, but also the dark-formed one; (2) ethylene emission is not affected by R or FR, and also not correlated with the hook exaggerations; thus ethylene is not responsible for the hook exaggeration in tomato; and (3) auxin is

Determination of the acute toxicity of isoniazid to three invasive carp species and rainbow trout in static exposures

USGS Publications Warehouse

Schreier, Theresa M.; Hubert, Terrance D.

2015-01-01

Three invasive fishes of considerable concern to aquatic resource managers are the Hypophthalmichthys nobilis (bighead carp),Hypophthalmichthys molitrix (silver carp), and Ctenopharyngodon idella (grass carp), collectively known as Asian carps. There is a need for an effective chemical control agent for Asian carps. Isoniazid was identified as a potential toxicant for grass carp. The selective toxicity of isoniazid to grass carp was verified as a response to an anecdotal report received in 2013. In addition, the toxicity of isoniazid to bighead carp, silver carp, and Oncorhynchus mykiss (rainbow trout) was evaluated. Isoniazid was not toxic to grass carp at the reported anecdotal concentration, which was 13 milligrams per liter. Isoniazid (130 milligrams per liter) was not selectively toxic to bighead carp, silver carp, or grass carp when compared to rainbow trout.

Food consumption and growth rates of juvenile black carp fed natural and prepared feeds

USGS Publications Warehouse

Hodgins, Nathaniel C.; Schramm, Harold L.; Gerard, Patrick D.

2014-01-01

The introduced mollusciphagic black carp Mylopharyngodon piceus poses a significant threat to native mollusks in temperate waters throughout the northern hemisphere, but consumption rates necessary to estimate the magnitude of impact on mollusks have not been established. We measured food consumption and growth rates for small (77–245 g) and large (466–1,071 g) triploid black carp held individually under laboratory conditions at 20, 25, and 30°C. Daily consumption rates (g food · g wet weight fish−1·d−1·100) of black carp that received prepared feed increased with temperature (small black carp 1.39–1.71; large black carp 1.28–2.10), but temperature-related increases in specific growth rate (100[ln(final weight) - ln(initial weight)]/number of days) only occurred for the large black carp (small black carp −0.02 to 0.19; large black carp 0.16–0.65). Neither daily consumption rates (5.90–6.28) nor specific growth rates (0.05–0.24) differed among temperatures for small black carp fed live snails. The results of these laboratory feeding trials indicate food consumption rates can vary from 289.9 to 349.5 J·g−1·d−1 for 150 g black carp receiving prepared feed, from 268.8 to 441.0 J·g−1·d−1for 800 g black carp receiving prepared feed, and from 84.8 to 90.2 J·g−1·d−1 for 150 g black carp that feed on snails. Applying estimated daily consumption rates to estimated biomass of native mollusks indicates that a relatively low biomass of bla

A Pedagogy of Sight: Microscopic Vision in Robert Hooke's "Micrographia"

ERIC Educational Resources Information Center

Jack, Jordynn

2009-01-01

Robert Hooke's "Micrographia" (1665) holds an important place in the history of scientific visual rhetoric. Hooke's accomplishment lies not only in a stunning array of engravings, but also in a "pedagogy of sight"--a rhetorical framework that instructs readers how to view images in accordance with an ideological or epistemic program. Hooke not…

Sidney Hook's Pragmatic Anti-Communism: Commitment to Democracy as Method

ERIC Educational Resources Information Center

Ferriter, Courtney

2017-01-01

In recent years, opposition to Communism has emerged as Sidney Hook's central philosophical legacy in the eyes of scholars and historians, who tend to ignore all of Hook's pre-Cold War philosophical contributions. Furthermore, critics who treat Hook's anti-Communism often accuse him of abandoning pragmatism for dogmatism in his later career. In…

Diagnosis and management of hook of hamate fractures.

PubMed

Kadar, Assaf; Bishop, Allen T; Suchyta, Marissa A; Moran, Steven L

2018-06-01

The purpose of this study was to evaluate the time to diagnosis and management of hook of hamate fractures in an era of advanced imaging. We performed a retrospective study of 51 patients treated for hook of hamate fractures. Patients were sent a quickDASH questionnaire regarding the outcomes of their treatment. Hook of hamate fractures were diagnosed with advanced imaging at a median of 27 days. Clinical findings of hook of hamate tenderness had better sensitivity than carpal tunnel-view radiographs. Nonunion occurred in 24% of patients with non-operative treatment and did not occur in the operative group. Both treatment groups achieved good clinical results, with a grip strength of 80% compared with the non-injured hand and a median quickDASH score of 2. Advanced imaging improved the time to diagnosis and treatment compared to historical case series. Nonunion is common in patients treated non-operatively. IV.

76 FR 11961 - Safety Zone, Dredging Operations; Delaware River, Marcus Hook, PA

Federal Register 2010, 2011, 2012, 2013, 2014

2011-03-04

...-AA00 Safety Zone, Dredging Operations; Delaware River, Marcus Hook, PA AGENCY: Coast Guard, DHS. ACTION... Delaware River while the Dredge Pullen conducts dredging operations at the Sunoco Marcus Hook docks in the vicinity of the Marcus Hook Range near Marcus Hook, PA. This action is necessary to maintain the 42 ft...

Education as a Practice of Freedom: Reflections on bell hooks

ERIC Educational Resources Information Center

Specia, Akello; Osman, Ahmed A.

2015-01-01

This paper critically analyses the conceptions of bell hooks on education. It focuses on the relevance of hook's ideas to the classroom. It is a theoretical paper based on secondary data that seeks to contribute to the growing body of knowledge in education. The paper is a reflection of hook's reaction to education as a practice of freedom, the…

Intra‐annual variability of Silver Carp populations in the Des Moines River, USA

USGS Publications Warehouse

Sullivan, Christopher J.; Camacho, Carlos A.; Weber, Michael J.; Pierce, Clay

2017-01-01

Since their introduction in the 1970s, Silver Carp Hypophthalmichthys molitrix have spread throughout the Mississippi River basin. Management of any species relies on an accurate understanding of population characteristics and dynamics. However, Silver Carp seasonal sampling variation is unknown. Sampling during periods of peak catch rates would facilitate Silver Carp assessment and management, improving monitoring and removal techniques. The objective of this study was to evaluate adult Silver Carp seasonal sampling variation with boat electroshocking and trammel nets. Silver Carp were collected monthly (April–October) during 2014 and 2015 from four locations in the Des Moines River, Iowa. Trammel nets rarely captured Silver Carp (mean ± SE = 4.9 ± 1.6 fish/net; 60% of fish were captured in 6.3% of net sets) and therefore were not included in analyses. Electroshocking catch rates (CPUEs) exhibited a bimodal distribution, with peak CPUEs generally occurring in May, June, and September and lower catch rates observed during July and August. Catch rates were positively related to river discharge at upstream sites but not at downstream sites. Silver Carp size structure was similar among months and sites except at Cliffland, where fish were smaller during August and October compared to earlier in the year. Finally, Silver Carp condition peaked during April and May and decreased throughout the year except at Keokuk, where peaks were observed during both May and August. Although spatiotemporal variability was substantial, these results suggest that sampling of Silver Carp via electroshocking in May–June and September–October generally produces higher catch rates compared to July–August sampling and generates a more representative size structure. Using site‐specific knowledge, monitoring and surveillance programs could more effectively sample during these periods of high vulnerability and densities in order to manage the spread and impacts of Silver Carp at

Newton–Hooke-type symmetry of anisotropic oscillators

DOE Office of Scientific and Technical Information (OSTI.GOV)

Zhang, P.M., E-mail: zhpm@impcas.ac.cn; Horvathy, P.A., E-mail: horvathy@lmpt.univ-tours.fr; Laboratoire de Mathématiques et de Physique Théorique, Université de Tours

2013-06-15

Rotation-less Newton–Hooke-type symmetry, found recently in the Hill problem, and instrumental for explaining the center-of-mass decomposition, is generalized to an arbitrary anisotropic oscillator in the plane. Conversely, the latter system is shown, by the orbit method, to be the most general one with such a symmetry. Full Newton–Hooke symmetry is recovered in the isotropic case. Star escape from a galaxy is studied as an application. -- Highlights: ► Rotation-less Newton–Hooke (NH) symmetry is generalized to an arbitrary anisotropic oscillator. ► The orbit method is used to find the most general case for rotation-less NH symmetry. ► The NH symmetry ismore » decomposed into Heisenberg algebras based on chiral decomposition.« less

Common carp disrupt ecosystem structure and function through middle-out effects

USGS Publications Warehouse

Kaemingk, Mark A.; Jolley, Jeffrey C.; Paukert, Craig P.; Willis, David W.; Henderson, Kjetil R.; Holland, Richard S.; Wanner, Greg A.; Lindvall, Mark L.

2016-01-01

Middle-out effects or a combination of top-down and bottom-up processes create many theoretical and empirical challenges in the realm of trophic ecology. We propose using specific autecology or species trait (i.e. behavioural) information to help explain and understand trophic dynamics that may involve complicated and non-unidirectional trophic interactions. The common carp (Cyprinus carpio) served as our model species for whole-lake observational and experimental studies; four trophic levels were measured to assess common carp-mediated middle-out effects across multiple lakes. We hypothesised that common carp could influence aquatic ecosystems through multiple pathways (i.e. abiotic and biotic foraging, early life feeding, nutrient). Both studies revealed most trophic levels were affected by common carp, highlighting strong middle-out effects likely caused by common carp foraging activities and abiotic influence (i.e. sediment resuspension). The loss of water transparency, submersed vegetation and a shift in zooplankton dynamics were the strongest effects. Trophic levels furthest from direct pathway effects were also affected (fish life history traits). The present study demonstrates that common carp can exert substantial effects on ecosystem structure and function. Species capable of middle-out effects can greatly modify communities through a variety of available pathways and are not confined to traditional top-down or bottom-up processes.

In-depth proteomic analysis of carp (Cyprinus carpio L) spermatozoa.

PubMed

Dietrich, Mariola A; Arnold, Georg J; Fröhlich, Thomas; Ciereszko, Andrzej

2014-12-01

Using a combination of protein fractionation by one-dimensional gel electrophoresis and high performance liquid chromatography-electrospray ionization tandem mass spectrometry, we identified 348 proteins in carp spermatozoa, most of which were for the first time identified in fish. Dynein, tubulin, HSP90, HSP70, HSP60, adenosylhomocysteinase, NKEF-B, brain type creatine kinase, mitochondrial ATP synthase, and valosin containing enzyme represent high abundance proteins in carp spermatozoa. These proteins are functionally related to sperm motility and energy production as well as the protection of sperm against oxidative injury and stress. Moreover, carp spermatozoa are equipped with functionally diverse proteins involved in signal transduction, transcription, translation, protein turnover and transport. About 15% of proteins from carp spermatozoa identified here were also detected in seminal plasma which may be a result of leakage from spermatozoa into seminal plasma, adsorption of seminal plasma proteins on spermatozoa surface, and expression in both spermatozoa and cells secreting seminal plasma proteins. The availability of a catalog of carp sperm proteins provides substantial advances for an understanding of sperm function and for future development of molecular diagnostic tests of carp sperm quality, the evaluation of which is currently limited to certain parameters such as sperm count, morphology and motility or viability. The mass spectrometry data are available at ProteomeXchange with the dataset identifier PXD000877 (DOI: http://dx.doi.org/10.6019/PXD000877). Copyright © 2014 Elsevier Inc. All rights reserved.

Development of an antimycin-impregnated bait for controlling common carp

USGS Publications Warehouse

Rach, J.J.; Luoma, J.A.; Marking, L.L.

1994-01-01

The common carp Cyprinus carpio is a major problem for fisheries and wildlife managers because its feeding behavior causes degradation of valuable fish and waterfowl habitat. This study was designed to evaluate the effectiveness of an antimycin-impregnated bait for control of common carp. The toxic bait contained fish meal, a binder, antimycin, and water. The ingredients were mixed together and made into pellets. This bait was force-fed to common carp or administered in a pond environment, where fish voluntarily fed on the bait. The lowest lethal dose in the forcefeeding study was 0.346 mg antimycin/kg of fish and doses that exceeded 0.811 mg antimycin/kg were toxic to all fish. On three occasions, adult common carp held in 0.004-ha concrete ponds were offered 10 g of toxic bait containing 5.0, 7.5, and 10 mg antimycin/g of bait and the mean mortalities 96 h later were 21, 35, and 51%, respectively. Three tests were conducted in 0.04-ha earthen ponds each containing 100 adult common carp; these fish were offered 50 g of the toxic bait that contained 10 mg antimycin/g, and the mean mortalities (96 h) were 19, 32, and 74%. Toxic baits should be used in conjunction with other management techniques, and only when common carp are congregated and actively feeding, and when few nontarget bottom- feeding species are present.

An analysis of inputs cost for carp farming sector in 2001 in Iran.

PubMed

Salehi, Hassan

2007-11-01

Carp is widely sold and used in its fresh in Iran, however, recently a range of value additions may also be observed. It is essential to the sustainable development of a carp farm to know the production costs and their contribution. Warm-water fish farming is mainly based on common, silver, grass and bighead carp and the common carp and the three Chinese species are often reared in poly culture in Iran. Since, the 1970s carp farming has spread around the Caspian coast and farmed production reached a peak in 2006 with production of more than 73,400 tons. A study of production, costs and profitability of carp farming sector was carried out to help clarify carp production costs and their difference with location in 2001. A total of 101 farms from the three main carp farming provinces, Guilan, Mazandaran and Khuzestan were randomly selected, classified and studied. The results of the survey showed that the various producer provinces have different cost structures. Overall, feed and fertilizer with the highest level of variation accounted for 23% of total costs, followed by seed and labor and salary with 23 and 17%, respectively. On average, benefit-cost ratio and the rate of farm income were closely related to location. This result suggests that farmers practice more efficiently and have better conditions in Mazandaran, followed by Guilan province.

Genomic insight into the common carp (Cyprinus carpio) genome by sequencing analysis of BAC-end sequences

PubMed Central

2011-01-01

Background Common carp is one of the most important aquaculture teleost fish in the world. Common carp and other closely related Cyprinidae species provide over 30% aquaculture production in the world. However, common carp genomic resources are still relatively underdeveloped. BAC end sequences (BES) are important resources for genome research on BAC-anchored genetic marker development, linkage map and physical map integration, and whole genome sequence assembling and scaffolding. Result To develop such valuable resources in common carp (Cyprinus carpio), a total of 40,224 BAC clones were sequenced on both ends, generating 65,720 clean BES with an average read length of 647 bp after sequence processing, representing 42,522,168 bp or 2.5% of common carp genome. The first survey of common carp genome was conducted with various bioinformatics tools. The common carp genome contains over 17.3% of repetitive elements with GC content of 36.8% and 518 transposon ORFs. To identify and develop BAC-anchored microsatellite markers, a total of 13,581 microsatellites were detected from 10,355 BES. The coding region of 7,127 genes were recognized from 9,443 BES on 7,453 BACs, with 1,990 BACs have genes on both ends. To evaluate the similarity to the genome of closely related zebrafish, BES of common carp were aligned against zebrafish genome. A total of 39,335 BES of common carp have conserved homologs on zebrafish genome which demonstrated the high similarity between zebrafish and common carp genomes, indicating the feasibility of comparative mapping between zebrafish and common carp once we have physical map of common carp. Conclusion BAC end sequences are great resources for the first genome wide survey of common carp. The repetitive DNA was estimated to be approximate 28% of common carp genome, indicating the higher complexity of the genome. Comparative analysis had mapped around 40,000 BES to zebrafish genome and established over 3,100 microsyntenies, covering over 50% of

Suitability of Lake Erie for bigheaded carps based on bioenergetic models and remote sensing

USGS Publications Warehouse

Anderson, Karl R.; Chapman, Duane C.; Wynne, Timothy; Masagounder, Karthik; Paukert, Craig P.

2015-01-01

Algal blooms in the Great Lakes are a potential food source for silver carp (Hypophthalmichthys molitrix) and bighead carp (H. nobilis; together bigheaded carps). Understanding these blooms thus plays an important role in understanding the invasion potential of bigheaded carps. We used remote sensing imagery, temperatures, and improved species specific bioenergetics models to determine algal concentrations sufficient for adult bigheaded carps. Depending on water temperature we found that bigheaded carp require between 2 and 7 μg/L chlorophyll or between 0.3 and 1.26 × 105cells/mL Microcystis to maintain body weight. Algal concentrations in the western basin and shoreline were found to be commonly several times greater than the concentrations required for weight maintenance. The remote sensing images show that area of sufficient algal foods commonly encompassed several hundred square kilometers to several thousands of square kilometers when blooms form. From 2002 to 2011, mean algal concentrations increased 273%–411%. This indicates Lake Erie provides increasingly adequate planktonic algal food for bigheaded carps. The water temperatures and algal concentrations detected in Lake Erie from 2008 to 2012 support positive growth rates such that a 4 kg silver carp could gain between 19 and 57% of its body weight in a year. A 5 kg bighead carp modeled at the same water temperatures could gain 20–81% of their body weight in the same period. The remote sensing imagery and bioenergetic models suggest that bigheaded carps would not be food limited if they invaded Lake Erie.

Assessing impacts of introduced aquatic species: Grass carp in large systems

NASA Astrophysics Data System (ADS)

Bain, Mark B.

1993-03-01

Introduced species have created environmental benefits and unanticipated disasters so a priori assessments of species introductions are needed for environmental management. A checklist for assessing impacts of introduced species was developed from studies of introduced species and recommendations for planning introductions. Sterile, triploid grass carp ( Ctenopharyngodon idella) are just beginning to be used as a biocontrol agent for the management of aquatic vegetation in open waterways. Potential impacts of grass carp in open systems were identified by reviewing grass carp biology relative to the impact assessment checklist. The potential consequences of introduced grass carp were reviewed for one case study. The case study demonstrated that conclusions about potential impacts and monitoring needs can be made despite incomplete information and uncertainty. Indicators of environmental impact and vulnerability of host systems were grouped into six categories: population control, hybridization, diseases and parasites, habitat alterations, biological effects, and management issues. Triploid grass carp can significantly alter habitat and biological resources through the secondary effects of reductions in aquatic vegetation. Potential impacts and significant uncertainties involve fish dispersions from plant control areas, inability to control vegetation loss, loss of diverse plant communities and their dependent species, and conflicts with human use of the water resource. Adequate knowledge existed to assess most potential consequences of releasing large numbers of triploid grass carp in Guntersville Reservoir, Alabama. However, the assessment of potential impacts indicated that moderate, incremental stockings combined with monitoring of vegetation and biological resources are necessary to control the effects of grass carp and achieve desirable, intermediate plant densities.

Hook2 is involved in the morphogenesis of the primary cilium

PubMed Central

Baron Gaillard, Carole L.; Pallesi-Pocachard, Emilie; Massey-Harroche, Dominique; Richard, Fabrice; Arsanto, Jean-Pierre; Chauvin, Jean-Paul; Lecine, Patrick; Krämer, Helmut; Borg, Jean-Paul; Le Bivic, André

2011-01-01

Primary cilia originate from the centrosome and play essential roles in several cellular, developmental, and pathological processes, but the underlying mechanisms of ciliogenesis are not fully understood. Given the involvement of the adaptor protein Hook2 in centrosomal homeostasis and protein transport to pericentrosomal aggresomes, we explored its role in ciliogenesis. We found that in human retinal epithelial cells, Hook2 localizes at the Golgi apparatus and centrosome/basal body, a strategic partitioning for ciliogenesis. Of importance, Hook2 depletion disrupts ciliogenesis at a stage before the formation of the ciliary vesicle at the distal tip of the mother centriole. Using two hybrid and immunoprecipitation assays and a small interfering RNA strategy, we found that Hook2 interacts with and stabilizes pericentriolar material protein 1 (PCM1), which was reported to be essential for the recruitment of Rab8a, a GTPase that is believed to be crucial for membrane transport to the primary cilium. Of interest, GFP::Rab8a coimmunoprecipitates with endogenous Hook2 and PCM1. Finally, GFP::Rab8a can overcome Hook2 depletion, demonstrating a functional interaction between Hook2 and these two important regulators of ciliogenesis. The data indicate that Hook2 interacts with PCM1 in a complex that also contains Rab8a and regulates a limiting step required for further initiation of ciliogenesis after centriole maturation. PMID:21998199

Management of lactating sows during heat stress: effects of water drip, snout coolers, floor type and a high energy-density diet.

PubMed

McGlone, J J; Stansbury, W F; Tribble, L F

1988-04-01

Two experiments using 120 sows were conducted to determine the effects during heat stress of two floor types, snout coolers or a water drip system, and a high energy-density diet. During both studies, air temperature was maintained at or above 29 degrees C. Floor types included partially slotted concrete and plastic-coated, expanded metal. In Exp. 1, in addition to floor-type treatments, snout coolers were on or off and the water drip was on for 3 min each 10 min or off. Snout coolers increased (P less than .05) sow feed intake and decreased (P less than .05) sow lactation weight loss. Water drip increased (P less than .002) sow feed intake and reduced lactation weight loss. The drip X floor-type interaction was significant for most measures of piglet performance. Drip was beneficial for piglet weights when piglets were on plastic, whereas drip was detrimental to piglet performance while they were housed on concrete. In Exp. 2, two floor types, drip or no-drip and a high energy-density diet or control diet were examined during heat stress. The high energy-density diet reduced (P less than .01) sow feed intake but provided no measurable increase in piglet performance during heat stress. We conclude that water drip is an effective cooling technique for heat-stressed sows, especially when floors are plastic. Snout coolers, partial concrete slots and high energy-density diets provided only minor benefits to heat-stressed sows and were not of benefit to piglets nursing heat-stressed sows.

Hooking-Up, Religiosity, and Sexting Among College Students.

PubMed

Hall, Michael; Williams, Ronald D; Ford, M Allison; Cromeans, Erin Murphy; Bergman, Randall J

2016-07-28

The purpose of this study was to evaluate the mediation effect of sexting, and taking sexually suggestive photos on religiosity and hooking-up with three separate sexual outcomes. A web-based survey examined the relationship between religiosity and the three hooking-up outcomes among students reporting sexting or taking a sexually suggestive photo in the last 30 days (n = 231). Sexting, as well as taking sexually suggestive photos mediated the relationship between religiosity and hooking-up among females. Sexting may be initiated by females as a way to engage in a nonphysical sexual interaction, which ultimately predisposes them to a physical sexual outcome.

46 CFR 173.007 - Location of the hook load.

Code of Federal Regulations, 2010 CFR

2010-10-01

... PERTAINING TO VESSEL USE Lifting § 173.007 Location of the hook load. When doing the calculations required in this subpart, the hook load must be considered to be located at the head of the crane. ...

Reexamining the frequency range of hearing in silver (Hypophthalmichthys molitrix) and bighead (H. nobilis) carp

USGS Publications Warehouse

Vetter, Brooke J.; Brey, Marybeth; Meninger, Allen F.

2018-01-01

Silver (Hypophthalmichthys molitrix) and bighead (H. nobilis) carp (collectively bigheaded carp) are invasive fish that threaten aquatic ecosystems in the upper Midwest United States and the Laurentian Great Lakes. Controlling bigheaded carp is a priority of fisheries managers and one area of focus involves developing acoustic deterrents to prevent upstream migration. For an acoustic deterrent to be effective however, the hearing ability of bigheaded carp must be characterized. A previous study showed that bigheaded carp detected sound up to 3 kHz but this range is narrower than what has been reported for other ostariophysans. Therefore, silver and bighead carp frequency detection was evaluated in response to 100 Hz to 9 kHz using auditory evoked potentials (AEPs). AEPs were recorded from 100 Hz to 5 kHz. The lowest thresholds were at 500 Hz for both species (silver carp threshold: 80.6 ± 3.29 dB re 1 μPa SPLrms, bighead carp threshold: 90.5 ± 5.75 dB re 1 μPa SPLrms; mean ± SD). These results provide fisheries managers with better insight on effective acoustic stimuli for deterrent systems, however, to fully determine bigheaded carp hearing abilities, these results need to be compared with behavioral assessments.

Thermal and hydrologic suitability of Lake Erie and its major tributaries for spawning of Asian carps

USGS Publications Warehouse

Kocovsky, Patrick M.; Chapman, Duane C.; McKenna, James E.

2012-01-01

Bighead carp Hypophthalmichthys nobilis, silver carp H. molitrix, and grass carp Ctenopharyngodon idella (hereafter Asian carps) have expanded throughout the Mississippi River basin and threaten to invade Lakes Michigan and Erie. Adult bighead carp and grass carp have been captured in Lake Erie, but self-sustaining populations probably do not exist. We examined thermal conditions within Lake Erie to determine if Asian carps would mature, and to estimate time of year when fish would reach spawning condition. We also examined whether thermal and hydrologic conditions in the largest tributaries to western and central Lake Erie were suitable for spawning of Asian carps. We used length of undammed river, predicted summer temperatures, and predicted water velocity during flood events to determine whether sufficient lengths of river are available for spawning of Asian carps. Most rivers we examined have at least 100 km of passable river and summer temperatures suitable (> 21 C) for rapid incubation of eggs of Asian carps. Predicted water velocity and temperature were sufficient to ensure that incubating eggs, which drift in the water column, would hatch before reaching Lake Erie for most flood events in most rivers if spawned far enough upstream. The Maumee, Sandusky, and Grand Rivers were predicted to be the most likely to support spawning of Asian carps. The Black, Huron, Portage, and Vermilion Rivers were predicted to be less suitable. The weight of the evidence suggests that the largest western and central Lake Erie tributaries are thermally and hydrologically suitable to support spawning of Asian carps.

Interactions of light and ethylene in hypocotyl hook maintenance in Arabidopsis thaliana seedlings

NASA Technical Reports Server (NTRS)

Knee, E. M.; Hangarter, R. P.; Knee, M.

2000-01-01

Etiolated seedlings frequently display a hypocotyl or epicotyl hook which opens on exposure to light. Etylene has been shown to be necessary for maintenance of the hook in a number of plants in darkness. We investigated the interaction of ethylene and light in the regulation of hypocotyl hook opening in Arabidopsis thaliana. We found that hooks of Arabidopsis open in response to continuous red, far-red or blue light in the presence of up to 100 microliters l-1 ethylene. Thus a change in sensitivity to ethylene is likely to be responsible for hook opening in Arabidopsis, rather than a decrease in ethylene production in hook tissues. We used photomorphogenic mutants of Arabidopsis to demonstrate the involvement of both blue light and phytochrome photosensory systems in light-induced hook opening in the presence of ethylene. In addition we used ethylene mutants and inhibitors of ethylene action to investigate the role of ethylene in hook maintenance in seedlings grown in light and darkness.

Multistate models of bigheaded carps in the Illinois River reveal spatial dynamics of invasive species

USGS Publications Warehouse

Coulter, Alison A; Brey, Marybeth; Lubejko, Matthew; Kallis, Jahn L; Glover, David C.; Whitledge, Gregory W; Garvey, James E.

2018-01-01

Knowledge of the spatial distributions and dispersal characteristics of invasive species is necessary for managing the spread of highly mobile species, such as invasive bigheaded carps (Bighead Carp [Hypophthalmichthys nobilis] and Silver Carp [H. molitrix]). Management of invasive bigheaded carps in the Illinois River has focused on using man-made barriers and harvest to limit dispersal towards the Laurentian Great Lakes. Acoustic telemetry data were used to parameterize multistate models to examine the spatial dynamics of bigheaded carps in the Illinois River to 1) evaluate the effects of current dams on movement, 2) identify how individuals distribute among pools, and 3) gauge the effects of reductions in movement towards the invasion front. Multistate models estimated that movement was generally less likely among upper river pools (Starved Rock, Marseilles, and Dresden Island) than the lower river (La Grange and Peoria) which matched the pattern of gated vs. wicket style dams. Simulations using estimated movement probabilities indicated that Bighead Carp accumulate in La Grange Pool while Silver Carp accumulate in Alton Pool. Fewer Bighead Carp reached the upper river compared to Silver Carp during simulations. Reducing upstream movement probabilities (e.g., reduced propagule pressure) by ≥ 75% into any of the upper river pools could reduce upper river abundance with similar results regardless of location. Given bigheaded carp reproduction in the upper Illinois River is limited, reduced movement towards the invasion front coupled with removal of individuals reaching these areas could limit potential future dispersal towards the Great Lakes.

Analysis of koi herpesvirus latency in wild common carp and ornamental koi in Oregon, USA.

PubMed

Xu, Jia-Rong; Bently, Jennifer; Beck, Linda; Reed, Aimee; Miller-Morgan, Tim; Heidel, Jerry R; Kent, Michael L; Rockey, Daniel D; Jin, Ling

2013-02-01

Koi herpesvirus (KHV) infection is associated with high mortalities in both common carp (Cyprinus carpio carpio) and koi carp (Cyprinus carpio koi) worldwide. Although acute infection has been reported in both domestic and wild common carp, the status of KHV latent infection is largely unknown in wild common carp. To investigate whether KHV latency is present in wild common carp, the distribution of KHV latent infection was investigated in two geographically distinct populations of wild common carp in Oregon, as well as in koi from an Oregon-based commercial supplier. Latent KHV infection was demonstrated in white blood cells from each of these populations. Although KHV isolated from acute infections has two distinct genetic groups, Asian and European, KHV detected in wild carp has not been genetically characterized. DNA sequences from ORF 25 to 26 that are unique between Asian and European were investigated in this study. KHV from captive koi and some wild common carp were found to have ORF-25-26 sequences similar to KHV-J (Asian), while the majority of KHV DNA detected in wild common carp has similarity to KHV-U/-I (European). In addition, DNA sequences from IL-10, and TNFR were sequenced and compared with no differences found, which suggests immune suppressor genes of KHV are conserved between KHV in wild common carp and koi, and is consistent with KHV-U, -I, -J. Copyright © 2012 Elsevier B.V. All rights reserved.

Factors regulating year‐class strength of Silver Carp throughout the Mississippi River basin

USGS Publications Warehouse

Sullivan, Christopher J.; Weber, Michael J.; Pierce, Clay; Wahl, David H.; Phelps, Quinton E.; Camacho, Carlos A.; Colombo, Robert E.

2018-01-01

Recruitment of many fish populations is inherently highly variable inter‐annually. However, this variability can be synchronous at broad geographic scales due to fish dispersal and climatic conditions. Herein, we investigated recruitment synchrony of Silver Carp Hypophthalmichthys molitrix across the Mississippi River basin. Year‐class strength (YCS) and synchrony of nine populations (max linear distance = 806.4 km) was indexed using catch‐curve residuals correlated between sites and related to local and regional climatic conditions. Overall, Silver Carp YCS was not synchronous among populations, suggesting local environmental factors are more important determinants of YCS than large‐scale environmental factors. Variation in Silver Carp YCS was influenced by river base flow and discharge variability at each site, indicating that extended periods of static local discharge benefit YCS. Further, river discharge and air temperature were correlated and synchronized among sites, but only similarities in river discharge was correlated with Silver Carp population synchrony, indicating that similarities in discharge (i.e., major flood) among sites can positively synchronize Silver Carp YCS. The positive correlation between Silver Carp YCS and river discharge synchrony suggests that regional flood regimes are an important force determining the degree of population synchrony among Mississippi River Silver Carp populations.

Structure, organization and expression of common carp (Cyprinus carpio L.) SLP-76 gene.

PubMed

Huang, Rong; Sun, Xiao-Feng; Hu, Wei; Wang, Ya-Ping; Guo, Qiong-Lin

2008-05-01

SLP-76 is an important member of the SLP-76 family of adapters, and it plays a key role in TCR signaling and T cell function. Partial cDNA sequence of SLP-76 of common carp (Cyprinus carpio L.) was isolated from thymus cDNA library by the method of suppression subtractive hybridization (SSH). Subsequently, the full length cDNA of carp SLP-76 was obtained by means of 3' RACE and 5' RACE, respectively. The full length cDNA of carp SLP-76 was 2007 bp, consisting of a 5'-terminal untranslated region (UTR) of 285 bp, a 3'-terminal UTR of 240 bp, and an open reading frame of 1482 bp. Sequence comparison showed that the deduced amino acid sequence of carp SLP-76 had an overall similarity of 34-73% to that of other species homologues, and it was composed of an NH2-terminal domain, a central proline-rich domain, and a C-terminal SH2 domain. Amino acid sequence analysis indicated the existence of a Gads binding site R-X-X-K, a 10-aa-long sequence which binds to the SH3 domain of LCK in vitro, and three conserved tyrosine-containing sequence in the NH2-terminal domain. Then we used PCR to obtain a genomic DNA which covers the entire coding region of carp SLP-76. In the 9.2k-long genomic sequence, twenty one exons and twenty introns were identified. RT-PCR results showed that carp SLP-76 was expressed predominantly in hematopoietic tissues, and was upregulated in thymus tissue of four-month carp compared to one-year old carp. RT-PCR and virtual northern hybridization results showed that carp SLP-76 was also upregulated in thymus tissue of GH transgenic carp at the age of four-months. These results suggest that the expression level of SLP-76 gene may be related to thymocyte development in teleosts.

Influence of sectioning location on age estimates from common carp dorsal spines

USGS Publications Warehouse

Watkins, Carson J.; Klein, Zachary B.; Terrazas, Marc M.; Quist, Michael C.

2015-01-01

Dorsal spines have been shown to provide precise age estimates for Common CarpCyprinus carpio and are commonly used by management agencies to gain information on Common Carp populations. However, no previous studies have evaluated variation in the precision of age estimates obtained from different sectioning locations along Common Carp dorsal spines. We evaluated the precision, relative readability, and distribution of age estimates obtained from various sectioning locations along Common Carp dorsal spines. Dorsal spines from 192 Common Carp were sectioned at the base (section 1), immediately distal to the basal section (section 2), and at 25% (section 3), 50% (section 4), and 75% (section 5) of the total length of the dorsal spine. The exact agreement and within-1-year agreement among readers was highest and the coefficient of variation lowest for section 2. In general, age estimates derived from sections 2 and 3 had similar age distributions and displayed the highest concordance in age estimates with section 1. Our results indicate that sections taken at ≤ 25% of the total length of the dorsal spine can be easily interpreted and provide precise estimates of Common Carp age. The greater consistency in age estimates obtained from section 2 indicates that by using a standard sectioning location, fisheries scientists can expect age-based estimates of population metrics to be more comparable and thus more useful for understanding Common Carp population dynamics.

Morphological, histological and molecular characterization of three Myxobolus species (Cnidaria: Myxosporea) from silver carp Hypophthalmichthys molitrix Valenciennes and bighead carp Hypophthalmichthys nobilis Richardson in China.

PubMed

Zhang, Bo; Gu, Zemao; Liu, Yang

2018-05-01

Three Myxobolus species were obtained from silver carp Hypophthalmichthys molitrix Valenciennes and bighead carp Hypophthalmichthys nobilis Richardson in China. In the present study, we supplemented their taxonomic characteristics by the morphological, histological and molecular methods. Myxobolus kiuchowensis Chen in Chen et Ma, 1998 formed small ellipsoidal plasmodia in the intestinal wall of bighead carp. Its spores appeared asymmetrical obovate in frontal view and fusiform in lateral view. Tiny mamillary protrusion in the anterior of some spores was observed. Two pyriform polar capsules were unequal. Histologically, M. kiuchowensis infected the tunica muscularis of host intestine. Myxobolus abitus Li et Nie, 1973 formed sausage-like plasmodia in the gills of silver carp. Its spores appeared oblate in frontal view and fusiform in lateral view. Two pyriform polar capsules were unequal and an obvious inter-capsule appendix was observed. Histological examination revealed that M. abitus developed in the interlamellar-epithelium of host gills. Myxobolus pavlovskii (Akhmerov, 1954) Landsberg et Lom, 1991 formed sausage-like plasmodia both in the gills of silver carp and bighead carp. Spores of M. pavlovskii were proximate oval in frontal view and fusiform in lateral view. Two pyriform polar capsules were unequal. The BLAST search indicated the SSU rDNA sequences of M. kiuchowensis and M. abitus were not identical to any sequence, however, the SSU rDNA sequences of M. pavlovskii were identical to that of M. pavlovskii recorded previously. Phylogenetic analysis showed that the present three species robustly clustered together in Cyprinid group and Asia group. Copyright © 2018. Published by Elsevier B.V.

Engineering Evaluation of International Low Impact Docking System Latch Hooks

NASA Technical Reports Server (NTRS)

Martinez, J.; Patin, R.; Figert, J.

2013-01-01

The international Low Impact Docking System (iLIDS) provides a structural arrangement that allows for visiting vehicles to dock with the International Space Station (ISS) (Fig 1). The iLIDS docking units are mechanically joined together by a series of active and passive latch hooks. In order to preserve docking capability at the existing Russian docking interfaces, the iLIDS latch hooks are required to conform to the existing Russian design. The latch hooks are classified as being fail-safe. Since the latch hooks are fail-safe, the hooks are not fracture critical and a fatigue based service life assessment will satisfy the structural integrity requirements. Constant amplitude fatigue testing to failure on four sets of active/passive iLIDS latch hooks was performed at load magnitudes of 10, 11, and 12 kips. Failure analysis of the hook fatigue failures identified multi-site fatigue initiation that was effectively centered about the hook mid-plane (consistent with the 3D model results). The fatigue crack initiation distribution implies that the fatigue damage accumulation effectively results in a very low aspect ratio surface crack (which can be simulated as thru-thickness crack). Fatigue damage progression resulted in numerous close proximity fatigue crack initiation sites. It was not possible to determine if fatigue crack coalescence occurs during cyclic loading or as result of the fast fracture response. The presence of multiple fatigue crack initiation sites on different planes will result in the formation of ratchet marks as the cracks coalesce. Once the stable fatigue crack becomes unstable and the fast fracture advances across the remaining ligament and the plane stress condition at a free-surface will result in failure along a 45 deg. shear plane (slant fracture) and the resulting inclined edge is called a shear lip. The hook thickness on the plane of fatigue crack initiation is 0.787". The distance between the shear lips on this plane was on the order of 0

Dynamic infrared imaging analysis of apical hook development in Arabidopsis: the case of brassinosteroids.

PubMed

Smet, Dajo; Žádníková, Petra; Vandenbussche, Filip; Benková, Eva; Van Der Straeten, Dominique

2014-06-01

Germination of Arabidopsis seeds in darkness induces apical hook development, based on a tightly regulated differential growth coordinated by a multiple hormone cross-talk. Here, we endeavoured to clarify the function of brassinosteroids (BRs) and cross-talk with ethylene in hook development. An automated infrared imaging system was developed to study the kinetics of hook development in etiolated Arabidopsis seedlings. To ascertain the photomorphogenic control of hook opening, the system was equipped with an automatic light dimmer. We demonstrate that ethylene and BRs are indispensable for hook formation and maintenance. Ethylene regulation of hook formation functions partly through BRs, with BR feedback inhibition of ethylene action. Conversely, BR-mediated extension of hook maintenance functions partly through ethylene. Furthermore, we revealed that a short light pulse is sufficient to induce rapid hook opening. Our dynamic infrared imaging system allows high-resolution, kinetic imaging of up to 112 seedlings in a single experimental run. At this high throughput, it is ideally suited to rapidly gain insight in pathway networks. We demonstrate that BRs and ethylene cooperatively regulate apical hook development in a phase-dependent manner. Furthermore, we show that light is a predominant regulator of hook opening, inhibiting ethylene- and BR-mediated postponement of hook opening. © 2014 The Authors. New Phytologist © 2014 New Phytologist Trust.

Carp lemonade

USGS Publications Warehouse

Chapman, Duane

2004-01-01

You've probably heard the adage, "When life gives you lemons, make lemonade!"  Missouri has been given some lemons in the form of bighead and silver carp.  These large, plankton eating fish, native to Asia, were imported into Arkansas in the 1970s to control water quality in fish farms and sewage treatment facilities.  It didn't take long for the fish to escape to the wild, and their populations have been increasing ever since.

Evaluation of environmental contaminants and elements in bigheaded carps of the Missouri River at Easley, Missouri, USA

USGS Publications Warehouse

Orazio, Carl E.; Chapman, Duane C.; May, Thomas W.; Meadows, John C.; Walther, Michael J.; Echols, Kathy R.; Deters, Joseph E.; Dierenfeld, S; Chapman, Duane C.; Hoff, Michael H.

2011-01-01

This book examines the history, biology, and status of Asian carps, and reviews current research on control measures. Explores the factors influencing recruitment and spread of Asian carps, considers current research on habitat requirements of bigheaded carps, analyzes data on diet overlap and potential competition between bigheaded carps and native fishes, and examines the use of pheromones as controls for Asian carps.

Mechanics of snout expansion in suction-feeding seahorses: musculoskeletal force transmission.

PubMed

Van Wassenbergh, Sam; Leysen, Heleen; Adriaens, Dominique; Aerts, Peter

2013-02-01

Seahorses and other syngnathid fishes rely on a widening of the snout to create the buccal volume increase needed to suck prey into the mouth. This snout widening is caused by abduction of the suspensoria, the long and flat bones outlining the lateral sides of the mouth cavity. However, it remains unknown how seahorses can generate a forceful abduction of the suspensoria. To understand how force is transmitted to the suspensoria via the hyoid and the lower jaw, we performed mathematical simulations with models based on computerized tomography scans of Hippocampus reidi. Our results show that the hinge joint between the left and right hyoid bars, as observed in H. reidi, allows for an efficient force transmission to the suspensorium from a wide range of hyoid angles, including the extremely retracted hyoid orientations observed in vivo for syngnathids. Apart from the hyoid retraction force by the sternohyoideus-hypaxial muscles, force generated in the opposite direction on the hyoid by the mandibulohyoid ligament also has an important contribution to suspensorium abduction torque. Forces on the lower jaw contribute only approximately 10% of the total suspensorium torque. In particular, when dynamical aspects of hyoid retraction are included in the model, a steep increase is shown in suspensorium abduction torque at highly retracted hyoid positions, when the linkages to the lower jaw counteract further hyoid rotation in the sagittal plane. A delayed strain in these linkages allows syngnathids to postpone suction generation until the end of cranial rotation, a fundamental difference from non-syngnathiform fishes.

Multistate models of bigheaded carps in the Illinois River reveal spatial dynamics of invasive species

USGS Publications Warehouse

Coulter, Alison A.; Brey, Marybeth; Lubejko, Matthew; Kallis, Jahn L.; Coulter, David P.; Glover, David C.; Whitledge, Gregory W.; Garvey, James E.

2018-01-01

Knowledge of the spatial distributions and dispersal characteristics of invasive species is necessary for managing the spread of highly mobile species, such as invasive bigheaded carps (Bighead Carp [Hypophthalmichthys nobilis] and Silver Carp [H. molitrix]). Management of invasive bigheaded carps in the Illinois River has focused on using human-made barriers and harvest to limit dispersal towards the Laurentian Great Lakes. Acoustic telemetry data were used to parameterize multistate models to examine the spatial dynamics of bigheaded carps in the Illinois River to (1) evaluate the effects of existing dams on movement, (2) identify how individuals distribute among pools, and (3) gauge the effects of reductions in movement towards the invasion front. Multistate models estimated that movement was generally less likely among upper river pools (Starved Rock, Marseilles, and Dresden Island) than the lower river (La Grange and Peoria) which matched the pattern of gated versus wicket style dams. Simulations using estimated movement probabilities indicated that Bighead Carp accumulate in La Grange Pool while Silver Carp accumulate in Alton Pool. Fewer Bighead Carp reached the upper river compared to Silver Carp during simulations. Reducing upstream movement probabilities (e.g., reduced propagule pressure) by ≥ 75% into any of the upper river pools could reduce upper river abundance with similar results regardless of location. Given bigheaded carp reproduction in the upper Illinois River is presently limited, reduced movement towards the invasion front coupled with removal of individuals reaching these areas could limit potential future dispersal towards the Great Lakes.

Phenotypic plasticity in the spawning traits of bigheaded carp (Hypophthalmichthys spp.) in novel ecosystems

USGS Publications Warehouse

Coulter, Alison A.; Keller, Doug; Amberg, Jon J.; Bailey, Elizabeth J.; Goforth, Reuben R.

2013-01-01

1. Bigheaded carp, including both silver (Hypophthalmichthys molitrix) and bighead (H. nobilis) carp, are successful invasive fishes that threaten global freshwater biodiversity. High phenotypic plasticity probably contributes to their success in novel ecosystems, although evidence of plasticity in several spawning traits has hitherto been largely anecdotal or speculative. 2. We collected drifting eggs from a Midwestern U.S.A. river from June to September 2011 and from April to June 2012 to investigate the spawning traits of bigheaded carp in novel ecosystems. 3. Unlike reports from the native range, the presence of drifting bigheaded carp eggs was not related to changes in hydrological regime or mean daily water temperature. Bigheaded carp also exhibited protracted spawning, since we found drifting eggs throughout the summer and as late as 1 September 2011. Finally, we detected bigheaded carp eggs in a river reach where the channel is c. 30 m wide with a catchment area of 4579 km2, the smallest stream in which spawning has yet been documented. 4. Taken with previous observations of spawning traits that depart from those observed within the native ranges of both bighead and silver carp, our findings provide direct evidence that bigheaded carp exhibit plastic spawning traits in novel ecosystems that may facilitate invasion and establishment in a wider range of river conditions than previously envisaged.

Dietary exposure of mink to carp from Saginaw Bay, Michigan: 2. Hematology and liver pathology

USGS Publications Warehouse

Heaton, S. N.; Bursian, S.J.; Giesy, J.P.; Tillitt, D. E.; Render, J. A.; Jones, P.D.; Verbrugge, D.A.; Kubiak, T.J.; Aulerich, R.J.

1995-01-01

The effects of consumption of environmental contaminants contained in carp (Cyprinus carpio)from Saginaw Bay, Michigan on various hematological parameters and liver integrity of adult female mink (Mustela vison) were determined. Mink were fed diets that contained 0 (control), 10, 20, or 40% carp prior to and throughout the reproductive period (182 days). The diets contained 0.015, 0.72, 1.53, and 2.56 mg polychlorinated biphenyls (PCBs)/kg diet and 1.0, 19, 40, and 81 pg 2,3,7,8-tetrachlorodibenzo-p-dioxin equivalents (TEQs)/g diet, respectively. Mink fed the diets containing carp showed a general dose-dependent occurrence of clinical signs commonly associated with chlorinated hydrocarbon toxicity, including listlessness, nervousness when approached, anorexia, and melena. Erythrocyte counts were less in mink exposed to Saginaw Bay carp than in controls, while the number of white blood cells was greater than in controls. Significant differences (p<0.05) in the concentrations of neutrophils, lymphocytes, monocytes, and eosinophils were also found between the control and carp-fed groups, but are considered to be of limited clinical or biological importance. Hematocrit values for the mink fed the 20 and 40% carp diets were significantly less than those of mink in the control and 10% carp groups. There were no significant differences in hemoglobin concentrations among the groups. Necropsies revealed enlarged yellowish livers in many of the carp-fed mink, especially those fed the 40% carp diet. Liver, spleen, and lung weights of carp-fed mink were significantly greater than those of control mink. Histopathologic examination of the livers revealed various degrees of congestion, hepatocellular fatty changes, and scattered portal lymphocytic infiltration which were most prevalent in mink fed the carp diets. These clinical signs, hematological effects, and histologic alterations are similar to those previously described for chlorinated hydrocarbon toxicoses in mink.

Assessment of dreissenid biodeposits as a potential food resource for invasive Asian carp

USGS Publications Warehouse

Anderson, Karl R.; Chapman, Duane C.; Hayer, Cari-Ann

2016-01-01

Silver carp (Hypophthalmichthys molitrix) and bighead carp (H. nobilis) are poised to invade the Laurentian Great Lakes. Zebra mussels (Dreissena polymorpha) and quagga mussels (D. rostriformis bugensis) have shifted nutrient pathways towards the benthos, partly through deposition of feces and rejected food particles called biodeposits. When biodeposit material was fed to bighead and silver carp, they fed on the material, but on average lost weight. Energy density between fed and unfed fish did not differ, but a few individual fish did gain weight on the biodeposits diet. Our results demonstrate that biodeposits might be considered a supplemental food for bigheaded carps.

75 FR 4265 - Airworthiness Directives; Lifesavings Systems Corp., D-Lok Hook Assembly

Federal Register 2010, 2011, 2012, 2013, 2014

2010-01-27

... Airworthiness Directives; Lifesavings Systems Corp., D-Lok Hook Assembly AGENCY: Federal Aviation Administration... adopting a new airworthiness directive (AD) for the Lifesavings Systems Corp., D-Lok Hook assembly... reported surface irregularities and discontinuities on certain D-Lok Hooks because of an unapproved change...

The Effect of Exposure to a High-Fat Diet on MicroRNA Expression in the Liver of Blunt Snout Bream (Megalobrama amblycephala)

PubMed Central

Zhang, Dingdong; Lu, Kangle; Dong, Zaijie; Jiang, Guangzhen; Xu, Weina; Liu, Wenbin

2014-01-01

Blunt snout bream (Megalobrama amblycephala) are susceptible to hepatic steatosis when maintained in modern intensive culture systems. The aim of this study was to investigate the potential roles of microRNAs (miRNAs) in diet-induced hepatic steatosis in this species. MiRNAs, small non-coding RNAs that regulate gene expression at the posttranscriptional level, are involved in diverse biological processes, including lipid metabolism. Deep sequencing of hepatic small RNA libraries from blunt snout bream fed normal-fat and high-fat diets identified 202 (193 known and 9 novel) miRNAs, of which 12 were differentially expressed between the normal-fat and high-fat diet groups. Quantitative stem-loop reverse transcriptase-polymerase chain reaction analyses confirmed the upregulation of miR-30c and miR-30e-3p and the downregulation of miR-145 and miR-15a-5p in high-fat diet-fed fish. Bioinformatics tools were used to predict the targets of these verified miRNAs and to explore potential downstream gene ontology biological process categories and Kyoto Encyclopedia of Genes and Genomes pathways. Six putative lipid metabolism-related target genes (fetuin-B, Cyp7a1, NADH dehydrogenase (ubiquinone) 1 beta subcomplex subunit 2, 3-oxoacid CoA transferase 1b, stearoyl-CoA desaturase, and fatty-acid synthase) were identified as having potential important roles in the development of diet-induced hepatic steatosis in blunt snout bream. The results presented here are a foundation for future studies of miRNA-controlled lipid metabolism regulatory networks in blunt snout bream. PMID:24788396

Biomechanical analysis of acromioclavicular joint dislocation treated with clavicle hook plates in different lengths.

PubMed

Shih, Cheng-Min; Huang, Kui-Chou; Pan, Chien-Chou; Lee, Cheng-Hung; Su, Kuo-Chih

2015-11-01

Clavicle hook plates are frequently used in clinical orthopaedics to treat acromioclavicular joint dislocation. However, patients often exhibit acromion osteolysis and per-implant fracture after undergoing hook plate fixation. With the intent of avoiding future complications or fixation failure after clavicle hook plate fixation, we used finite element analysis (FEA) to investigate the biomechanics of clavicle hook plates of different materials and sizes when used in treating acromioclavicular joint dislocation. Using finite element analysis, this study constructed a model comprising four parts: clavicle, acromion, clavicle hook plate and screws, and used the model to simulate implanting different types of clavicle hook plates in patients with acromioclavicular joint dislocation. Then, the biomechanics of stainless steel and titanium alloy clavicle hook plates containing either six or eight screw holes were investigated. The results indicated that using a longer clavicle hook plate decreased the stress value in the clavicle, and mitigated the force that clavicle hook plates exert on the acromion. Using a clavicle hook plate material characterized by a smaller Young's modulus caused a slight increase in the stress on the clavicle. However, the external force the material imposed on the acromion was less than the force exerted on the clavicle. The findings of this study can serve as a reference to help orthopaedic surgeons select clavicle hook plates.

Molecular cloning of the MARCH family in grass carp (Ctenopharyngodon idellus) and their response to grass carp reovirus challenge.

PubMed

Ou, Mi; Huang, Rong; Xiong, Lv; Luo, Lifei; Chen, Geng; Liao, Lanjie; Li, Yongming; He, Libo; Zhu, Zuoyan; Wang, Yaping

2017-04-01

Grass carp (Ctenopharyngodon idellus) is an economical aquaculture species in China, and the Grass Carp Reovirus (GCRV) that causes hemorrhagic disease seriously affects the grass carp cultivation industry. Substantial evidence indicates that there is an association between the membrane-associated RING-CH family of E3 ligase (MARCH) family and immune defense in mammals, while functional studies on non-mammalian MARCH proteins are limited. In order to know the characteristics of the MARCH genes in C. idellus, eight MARCH genes (MARCH1, 2, 5, 6, 7, 8, 9 and 11) were cloned and the open reading frames (ORF) were identified in grass carp. All MARCH proteins in grass carp contained an RING-CH domain, which is characteristic of the MARCH protein. The phylogenetic analysis revealed that different MARCH proteins gathered into their separate clusters. All eight members of the MARCH gene family were detected in all tissues sampled, but the relative expression level differed. In addition, the mRNA expression of all the MARCHs was regulated at different levels in the immune organs after a GCRV challenge, and they responded robustly in both the intestine and liver. The mRNA expression of MARCH8, MHC II, TfR, IL1RAP, EGR1, and DUSP1 in the intestine after GCRV infection was analyzed, and the results showed that MARCH8 could negatively regulate TfR, IL1RAP, EGR1, and DUSP1, which signaled via the MAPK or NF-κB-activation pathways that play vital roles in immunity. Our findings identified a novel gene family in C. idellus and provided novel evidence that MARCH genes are inducible and involved in the immune response. Moreover, MARCH8 might function to negatively regulate immune receptors in C. idellus. Therefore, the MARCH might play a vital role in regulating the immune response of C. idellus. Copyright © 2017 Elsevier Ltd. All rights reserved.

Aquacultural and socio-economic aspects of processing carps into some value-added products.

PubMed

Sehgal, H S; Sehgal, G K

2002-05-01

Carps are the mainstay of Indian aquaculture, contributing over 90% to the total fish production, which was estimated to be 1.77 million metric tonnes in 1996. Carp culture has a great potential for waste utilization and thus for pollution abatement. Many wastes such as cow, poultry, pig, duck, goat, and sheep excreta, biogas slurry, effluents from different kinds of factories/industries have been efficiently used for enhancing the productivity of natural food of carps and related species. Besides, several organic wastes/byproducts such as plant products, wastes from animal husbandry, and industrial by-products have been used as carp feed ingredients to lower the cost of supplementary feeding. However, to ensure the continued expansion of fish ponds and the pollution control, there must be a market for the fish (carps) produced in these ponds. The carps have, however, a low market value due to the presence of intra-muscular bones, which reduces their consumer acceptability. Thus, a need was felt to develop some boneless convenience products for enhancing the consumer acceptability of the carps. Efforts were made to prepare three value-added fish products, namely fish patty, fish finger and fish salad from carp flesh and were compared with a reference product ('fish pakoura'). Sensory evaluation of these products gave highly encouraging results. The methods of preparation of these products were transferred to some progressive farmers of the region who prepared and sold these products at very attractive prices. Carp processing has a great potential for the establishment of a fish ancillary industry and thus for boosting the production of these species. In Punjab alone, there is a potential of consuming 32,448 metric tonnes per annum of such value-added products (which would require 54,080 metric tonnes of raw fish). The development of value-added products has a significant role in raising the socio-economic status of the people associated with carp culture. The

Kinematics of Hooke universal joint robot wrists

NASA Technical Reports Server (NTRS)

Mckinney, William S., Jr.

1988-01-01

The singularity problem associated with wrist mechanisms commonly found on industrial manipulators can be alleviated by redesigning the wrist so that it functions as a three-axis gimbal system. This paper discussess the kinematics of gimbal robot wrists made of one and two Hooke universal joints. Derivations of the resolved rate motion control equations for the single and double Hooke universal joint wrists are presented using the three-axis gimbal system as a theoretical wrist model.

Interdependence of the rad50 hook and globular domain functions.

PubMed

Hohl, Marcel; Kochańczyk, Tomasz; Tous, Cristina; Aguilera, Andrés; Krężel, Artur; Petrini, John H J

2015-02-05

Rad50 contains a conserved Zn(2+) coordination domain (the Rad50 hook) that functions as a homodimerization interface. Hook ablation phenocopies Rad50 deficiency in all respects. Here, we focused on rad50 mutations flanking the Zn(2+)-coordinating hook cysteines. These mutants impaired hook-mediated dimerization, but recombination between sister chromatids was largely unaffected. This may reflect that cohesin-mediated sister chromatid interactions are sufficient for double-strand break repair. However, Mre11 complex functions specified by the globular domain, including Tel1 (ATM) activation, nonhomologous end joining, and DNA double-strand break end resection were affected, suggesting that dimerization exerts a broad influence on Mre11 complex function. These phenotypes were suppressed by mutations within the coiled-coil and globular ATPase domains, suggesting a model in which conformational changes in the hook and globular domains are transmitted via the extended coils of Rad50. We propose that transmission of spatial information in this manner underlies the regulation of Mre11 complex functions. Copyright © 2015 Elsevier Inc. All rights reserved.

Abnormal cerebellar development and ataxia in CARP VIII morphant zebrafish.

PubMed

Aspatwar, Ashok; Tolvanen, Martti E E; Jokitalo, Eija; Parikka, Mataleena; Ortutay, Csaba; Harjula, Sanna-Kaisa E; Rämet, Mika; Vihinen, Mauno; Parkkila, Seppo

2013-02-01

Congenital ataxia and mental retardation are mainly caused by variations in the genes that affect brain development. Recent reports have shown that mutations in the CA8 gene are associated with mental retardation and ataxia in humans and ataxia in mice. The gene product, carbonic anhydrase-related protein VIII (CARP VIII), is predominantly present in cerebellar Purkinje cells, where it interacts with the inositol 1,4,5-trisphosphate receptor type 1, a calcium channel. In this study, we investigated the effects of the loss of function of CARP VIII during embryonic development in zebrafish using antisense morpholino oligonucleotides against the CA8 gene. Knockdown of CA8 in zebrafish larvae resulted in a curved body axis, pericardial edema and abnormal movement patterns. Histologic examination revealed gross morphologic defects in the cerebellar region and in the muscle. Electron microscopy studies showed increased neuronal cell death in developing larvae injected with CA8 antisense morpholinos. These data suggest a pivotal role for CARP VIII during embryonic development. Furthermore, suppression of CA8 expression leads to defects in motor and coordination functions, mimicking the ataxic human phenotype. This work reveals an evolutionarily conserved function of CARP VIII in brain development and introduces a novel zebrafish model in which to investigate the mechanisms of CARP VIII-related ataxia and mental retardation in humans.

Do wild fish species contribute to the transmission of koi herpesvirus to carp in hatchery ponds?

PubMed

Fabian, M; Baumer, A; Steinhagen, D

2013-05-01

The koi herpesvirus (KHV) has spread worldwide since its discovery in 1998 and causes disease and mortality in koi and common carp populations with a high impact on the carp production industry. Many investigations have been conducted to examine ways of distribution and to identify possible transmission vectors. The answers, however, raise many new questions. In the present study, different wild fish species taken from carp ponds with a history of KHV infection were examined for their susceptibility to the virus. In the tissue of these fish, the virus load was determined and it was tested whether a release of the virus could be induced by stress and the virus then could be transferred to naive carp. Wild fish were gathered from carp ponds during acute outbreaks of virus-induced mortality in summer and from ponds stocked with carp carrying a latent KHV infection. From these ponds, wild fish were collected during the harvesting process in autumn or spring when the ponds were drained. We found that regardless of season, temperature variation, age and infection status of the carp stock, wild fish from carp ponds and its outlets could be tested positive for the KHV genome using real-time PCR with a low prevalence and virus load. Furthermore, virus transfer to naive carp was observed after a period of cohabitation. Cyprinid and non-cyprinid wild fish can therefore be considered as an epidemiological risk for pond carp farms. © 2012 Blackwell Publishing Ltd.

Modeling the hook depth distribution of pelagic longlining in the equatorial area of Indian Ocean

NASA Astrophysics Data System (ADS)

Song, Liming; Li, Jie; Gao, Panfeng; Zhou, Ji; Xu, Liuxiong

2012-12-01

A survey was conducted in the equatorial area of Indian Ocean for a better understanding of the dynamics of hook depth distribution of pelagic longline fishery. We determined the relationship between hook depth and vertical shear of current coefficiency, wind speed, hook position code, sine of wind angle, sine of angle of attack and weight of messenger weight. We identified the hook depth models by the analysis of covariance with a general linear model. The results showed that the wind effect on the hook depth can be ignored from October to November in the survey area; the surface current effect on the hook depth can be ignored; the equatorial undercurrent is the key factor for the hook depth in Indian Ocean; and there is a negative correlation between the hook depth and vertical shear of current and angle of attack. It was also found that the deeper the hook was set, the higher hook depth shoaling was. The proposed model improves the accuracy of the prediction of hook depth, which can be used to estimate the vertical distribution of pelagic fish in water column.

Ecological risk assessment of Grass Carp (Ctenopharyngodon idella) for the Great Lakes Basin

USGS Publications Warehouse

Kolar, Cynthia S.; Cudmore, Becky

2017-01-01

Grass Carp (Ctenopharyngodon idella) is an herbivorous, freshwater fish that was first introduced in the United States in the early 1960s for use in biological control of aquatic vegetation. It has since escaped and dispersed through the Mississippi River basin towards the Great Lakes. To characterize the risk of Grass Carp to the Great Lakes basin, a binational ecological risk assessment of Grass Carp was conducted.This risk assessment covered both triploid (sterile) and diploid (fertile) Grass Carp and assessed the likelihood of arrival, survival, establishment, and spread, and the magnitude of the ecological consequences within 5, 10, 20 and 50 years from 2014 (i.e., the baseline year) to the connected Great Lakes basin (defined as the Great Lakes basin and its tributaries to the first impassable barrier; risk was assessed based on current climate conditions and at the individual lake scale but does not address a finer geographical scale (e.g., bay or sub-region).For triploid Grass Carp, the probability of occurrence (likelihood of arrival, survival, and spread) was assessed, and for diploid Grass Carp the probability of introduction (likelihood of arrival, survival, establishment and spread) was assessed.

The dynamic instability in the hook/flagellum system that triggers bacterial flicks

NASA Astrophysics Data System (ADS)

Jabbarzadeh, Mehdi; Fu, Henry

2017-11-01

Dynamical bending, buckling, and polymorphic transformations of the flagellum are known to affect bacterial motility, but run-reverse-flick motility of monotrichous bacteria also involves the even more flexible hook, which connects the flagellum to the cell body. Here, we identify the dynamic buckling mechanism that produces flicks in Vibrio alginolyticus. Estimates of forces and torques on the hook from experimental observations suggest that flicks are triggered at stresses below the hook's static Euler buckling criterion. Using an accurate linearization of the Kirchoff rod model for the hook in a model of a swimming bacterium with rigid flagellum, we show that as hook stiffness decreases there is a transition from on-axis flagellar rotation with small hook deflections to flagellar precession with large deflections. When flagellum flexibility is incorporated, the precession is disrupted by significant flagellar bending - i.e., incipient flicks. The predicted onset of dynamic instabilities corresponds well with experimentally observed flick events.

Study on The Geopolymer Concrete Properties Reinforced with Hooked Steel Fiber

NASA Astrophysics Data System (ADS)

Abdullah, M. M. A. B.; Tahir, M. F. M.; Tajudin, M. A. F. M. A.; Ekaputri, J. J.; Bayuaji, R.; Khatim, N. A. M.

2017-11-01

In this research, Class F fly ash and a mixture of alkaline activators and different amount of hooked steel fiber were used for preparing geopolymer concrete. In order to analyses the effect of hooked steel fiber on the geopolymer concrete, the analysis such as chemical composition of fly ash, workability of fresh geopolymer, water absorption, density, compressive strength of hardened geopolymer concrete have been carried out. Mixtures were prepared with fly ash to alkaline liquid ratio of 2.0 with hooked steel fibers were added to the mix with different amounts which are 1%, 3%, 5% and 7% by the weight of the concrete. Experimental results showed that the compressive strength of geopolymer concrete increases as the hooked steel fibers increases. The optimum compressive strength obtained was up to 87.83 MPa on the 14th day. The density of geopolymer concrete are in the range between 2466 kg/m3 to 2501 kg/m3. In addition, the workability value of geopolymer without hooked steel fibers is 100 mm while the workability value of geopolymer with hooked steel fibers are between 60 mm to 30 mm.

A Model of Differential Growth-Guided Apical Hook Formation in Plants

PubMed Central

Žádníková, Petra; Wabnik, Krzysztof; Abuzeineh, Anas; Prusinkiewicz, Przemysław

2016-01-01

Differential cell growth enables flexible organ bending in the presence of environmental signals such as light or gravity. A prominent example of the developmental processes based on differential cell growth is the formation of the apical hook that protects the fragile shoot apical meristem when it breaks through the soil during germination. Here, we combined in silico and in vivo approaches to identify a minimal mechanism producing auxin gradient-guided differential growth during the establishment of the apical hook in the model plant Arabidopsis thaliana. Computer simulation models based on experimental data demonstrate that asymmetric expression of the PIN-FORMED auxin efflux carrier at the concave (inner) versus convex (outer) side of the hook suffices to establish an auxin maximum in the epidermis at the concave side of the apical hook. Furthermore, we propose a mechanism that translates this maximum into differential growth, and thus curvature, of the apical hook. Through a combination of experimental and in silico computational approaches, we have identified the individual contributions of differential cell elongation and proliferation to defining the apical hook and reveal the role of auxin-ethylene crosstalk in balancing these two processes. PMID:27754878

Effect of thiacloprid on early life stages of common carp (Cyprinus carpio).

PubMed

Velisek, Josef; Stara, Alzbeta

2018-03-01

Toxicity of the neonicotinoid pesticide thiacloprid to embryos and larvae of common carp Cyprinus carpio was assessed under laboratory conditions. Carp embryos and larvae were exposed to thiacloprid at four concentrations: 4.5 (environmental concentration), 45, 225, and 450 μg/L for 35 days and compared to a non-treated control group. The effects on lipid peroxidation, antioxidant enzyme levels (superoxide dismutase, glutathione reductase, catalase, glutathione S-transferase, and reduced glutathione), mortality, growth, development, and histopathogenesis were assessed. Thiacloprid at tested concentrations were not associated with negative effects on hatching, behaviour, embryo viability, or Fulton's condition factor. Carp exposed to 45 μg/L thiacloprid showed lower weight and length compared to controls. Thiacloprid at 225 and 450 μg/L delayed development and led to lower weight and length of carp. Significantly lower superoxide dismutase and glutathione reductase activity was observed at all tested concentrations of thiacloprid compared with the control group. Chronic thiacloprid exposure of early-life stages of carp affected ontogeny and growth rate, and inhibited antioxidant capacity. Copyright © 2017 Elsevier Ltd. All rights reserved.

Relationship between tornadoes and hook echoes on April 3, 1974

NASA Technical Reports Server (NTRS)

Forbes, G. S.

1975-01-01

Radar observations of tornado families occurring on April 3, 1974 are discussed. Of the 93 tornadoes included in the sample, 81% were associated with hook-like echoes with appendages at least 40 deg to the south of the echo movement. At least one tornado was associated with 62% of the hook-like echoes observed. All of the tornadoes with intensities of F 4 and F 5 were produced by hook-like echoes; the mean intensity of all tornadoes associated with this type of echo was F 3, while the mean intensity of the remaining tornadoes was F1. The tornadic hook-like echoes moved to the right of the non-tornadic echoes forming a tornado line in advance of the squall line. Some tornadoes were associated with 'spiral' echoes.

The complete mitochondrial genome of the Japanese ornamental koi carp (Cyprinus carpio) and its implication for the history of koi.

PubMed

Mabuchi, Kohji; Song, Hayeun

2014-02-01

Complete mitochondrial genome (mitogenome) sequences were determined for two individuals of Japanese ornamental koi carp. Interestingly, the obtained mitogenomes (16,581 bp) were both completely identical to the recently reported mitogenome of Oujiang color carp from China. Control region (CR) sequences in DNA database demonstrated that more than half (65%) of the koi carp individuals so far reported had partial or complete CR sequences identical to those from Oujiang color carp. These results might suggest that the Japanese koi carp has been originated from Chinese Oujiang color carp, contrary to the belief in Japan that the koi carps have been developed directly from carp stocks in Japan. In any case, the present results emphasize the importance of analyzing Oujiang color carp when studying the origin of koi carp.

Stainless hooks to bond lower lingual retainer.

PubMed

Durgekar, Sujala G; Nagaraj, K

2011-01-01

We introduced a simple and economical technique for precise placement of lower lingual retainers. Two stainless steel hooks made of 0.6mm wire are placed interdentally in the embrasure area between canine and lateral incisor bilaterally to lock the retainer wire in the correct position. Etch, rinse and dry the enamel surfaces with the retainer passively in place, then bond the retainer with light-cured adhesive. Hooks are simple to fabricate and eliminate the need for a transfer tray.

Transcriptome analysis reveals the time of the fourth round of genome duplication in common carp (Cyprinus carpio)

PubMed Central

2012-01-01

Background Common carp (Cyprinus carpio) is thought to have undergone one extra round of genome duplication compared to zebrafish. Transcriptome analysis has been used to study the existence and timing of genome duplication in species for which genome sequences are incomplete. Large-scale transcriptome data for the common carp genome should help reveal the timing of the additional duplication event. Results We have sequenced the transcriptome of common carp using 454 pyrosequencing. After assembling the 454 contigs and the published common carp sequences together, we obtained 49,669 contigs and identified genes using homology searches and an ab initio method. We identified 4,651 orthologous pairs between common carp and zebrafish and found 129,984 paralogous pairs within the common carp. An estimation of the synonymous substitution rate in the orthologous pairs indicated that common carp and zebrafish diverged 120 million years ago (MYA). We identified one round of genome duplication in common carp and estimated that it had occurred 5.6 to 11.3 MYA. In zebrafish, no genome duplication event after speciation was observed, suggesting that, compared to zebrafish, common carp had undergone an additional genome duplication event. We annotated the common carp contigs with Gene Ontology terms and KEGG pathways. Compared with zebrafish gene annotations, we found that a set of biological processes and pathways were enriched in common carp. Conclusions The assembled contigs helped us to estimate the time of the fourth-round of genome duplication in common carp. The resource that we have built as part of this study will help advance functional genomics and genome annotation studies in the future. PMID:22424280

Genetic variability comparison of cultured Israeli carp (Cyprinus carpio) from Korea using microsatellites.

PubMed

Kim, Jung Eun; Goo, In Bon; Hwang, Ju-Ae; Kim, Hyeong Su; Choi, Hye-Sung; Lee, Jeong-Ho

2018-06-01

In aquaculture, cultured fish often undergo continuous cross-fertilization without any inflow of new broodstock. This lowers genetic diversity, leading to increased disease rates and decreased survival rates. To improve the mass production and easy culture of Israeli carp, it is essential to investigate the population structure and genetic diversity of these fish. However, such a survey has not yet been performed on Korean Israeli carp. In this study, we used seven microsatellite markers to analyze the genetic diversity and association of cultured Israeli carp from Korea and China. The average numbers of alleles per locus (N A ) for two Korean (KorA and KorB) and two Chinese (ChA and ChB) populations were as follows: KorA (10.42), KorB (14.43), ChA (20.57) and ChB (20.71). The expected heterozygosity (H e ) ranged from 0.672 to 0.897 and from 0.827 to 0.938 in the Korean sample and Chinese sample respectively. The genetic diversity of the Korean Israeli carp was about half that of the Chinese carp. The diversity of the Korean Israeli carp was very low, suggesting that the immunity of this population could be weak, and that diversity-recovery studies are urgently needed. Therefore, our results may therefore form the foundation for future research efforts towards genetic monitoring and selective breeding, continuous research needs to be conducted in order to recover the genetic diversity of the Korean Israeli carp.

Carbon dioxide as a tool to deter the movement of invasive bigheaded carps

USGS Publications Warehouse

Michael R. Donaldson,; Amberg, Jon J.; Shivani Adhikari,; Cupp, Aaron R.; Jensen, Nathan; Romine, Jason G.; Adam Wright,; Gaikowski, Mark; Cory D. Suski,

2016-01-01

Nonnative bigheaded carps are established in the Mississippi River and there is substantial concern about their potential entry into the interconnected Laurentian Great Lakes. While electrical barriers currently exist as a preventative measure, there is need for additional control mechanisms to promote barrier security through redundancy. We tested the effectiveness of infused carbon dioxide gas (CO2) as a tool to influence the movement and behavior invasive bigheaded carps, namely Bighead Carp Hypophthalmichthys nobilis and Silver Carp H. molitrix, as well as native Bigmouth Buffalo Ictiobus cyprinellus, Channel Catfish Ictalurus punctatus, Paddlefish Polyodon spathula, and Yellow Perch Perca flavescens in an experimental pond. Individuals were monitored with acoustic telemetry before, during, and after CO2 addition to the pond. We noted distinct changes in fish behavior following CO2 addition. Each species except Paddlefish maintained farther distances from the CO2 infusion manifold relative to controls. Both bigheaded carp species had slower persistence velocities (persistence of a movement in a given direction) following CO2 infusion and Bighead Carp used a smaller area of the pond immediately after CO2 addition. Pond pH progressively decreased up to 1.5 units following CO2 infusion. This work provides evidence that could inform future research to enhance existing control measures used to deter high-risk invasive fishes, such as bigheaded carps.

Improving efficiency and reliability of environmental DNA analysis for silver carp

USGS Publications Warehouse

Amberg, Jon J.; McCalla, S. Grace; Monroe, Emy; Lance, Richard; Baerwaldt, Kelly; Gaikowski, Mark P.

2015-01-01

Natural resource agencies have established surveillance programs which use environmental DNA (eDNA) for the early detection of bighead carp Hypophthalmichthys nobilis and silver carp Hypophthalmichthys molitrix before they establish populations within the Great Lakes. This molecular monitoring technique must be highly accurate and precise for confident interpretation and also efficient, both in detection threshold and cost. Therefore, we compared two DNA extraction techniques and compared a new quantitative PCR (qPCR) assay with the conventional PCR (cPCR) assay used by monitoring programs. Both the qPCR and cPCR assays were able to amplify the DNA of silver carp present in environmental samples taken from locations where mixed populations of bigheaded carps existed. However, the qPCR assay had substantially fewer PCR positive samples which were subsequently determined not to contain DNA of bigheaded carps than the cPCR assay. Additionally, the qPCR assay was able to amplify the DNA of bigheaded carps even in the presence of inhibitors that blocked amplification with cPCR. Also, the selection of an appropriate DNA extraction method can significantly alter the efficiency of eDNA surveillance programs by lowering detection limits and by decreasing costs associated with sample processing. The results reported herein are presently being incorporated into eDNA surveillance programs to decrease the costs, increase DNA yield and increase the confidence that assays are amplifying the target DNA. These results are critical to enhancing our ability to accurately and confidently interpret the results reported from monitoring programs using eDNA for early detection of invasive species.

First evidence of grass carp recruitment in the Great Lakes Basin

USGS Publications Warehouse

Chapman, Duane C.; Davis, J. Jeremiah; Jenkins, Jill A.; Kocovsky, Patrick M.; Miner, Jeffrey G.; Farver, John; Jackson, P. Ryan

2013-01-01

We use aging techniques, ploidy analysis, and otolith microchemistry to assess whether four grass carp Ctenopharyngodon idella captured from the Sandusky River, Ohio were the result of natural reproduction within the Lake Erie Basin. All four fish were of age 1 +. Multiple lines of evidence indicate that these fish were not aquaculture-reared and that they were most likely the result of successful reproduction in the Sandusky River. First, at least two of the fish were diploid; diploid grass carp cannot legally be released in the Great Lakes Basin. Second, strontium:calcium (Sr:Ca) ratios were elevated in all four grass carp from the Sandusky River, with elevated Sr:Ca ratios throughout the otolith transect, compared to grass carp from Missouri and Arkansas ponds. This reflects the high Sr:Ca ratio of the Sandusky River, and indicates that these fish lived in a high-strontium environment throughout their entire lives. Third, Sandusky River fish were higher in Sr:Ca ratio variability than fish from ponds, reflecting the high but spatially and temporally variable strontium concentrations of southwestern Lake Erie tributaries, and not the stable environment of pond aquaculture. Fourth, Sr:Ca ratios in the grass carp from the Sandusky River were lower in their 2011 growth increment (a high water year) than the 2012 growth increment (a low water year), reflecting the observed inverse relationship between discharge and strontium concentration in these rivers. We conclude that these four grass carp captured from the Sandusky River are most likely the result of natural reproduction within the Lake Erie Basin.

Validation of eDNA surveillance sensitivity for detection of Asian carps in controlled and field experiments

USGS Publications Warehouse

Mahon, Andrew R.; Jerde, Christopher L.; Galaska, Matthew; Bergner, Jennifer L.; Chadderton, W. Lindsay; Lodge, David M.; Hunter, Margaret E.; Nico, Leo G.

2013-01-01

In many North American rivers, populations of multiple species of non-native cyprinid fishes are present, including black carp (Mylpharyngodon piceus), grass carp (Ctenopharyngodon idella), bighead carp (Hypophthalmichthys nobilis), silver carp (Hypophthalmichthys molitrix), common carp (Cyprinus carpio), and goldfish (Carassius auratus). All six of these species are found in the Mississippi River basin and tracking their invasion has proven difficult, particularly where abundance is low. Knowledge of the location of the invasion front is valuable to natural resource managers because future ecological and economic damages can be most effectively prevented when populations are low. To test the accuracy of environmental DNA (eDNA) as an early indicator of species occurrence and relative abundance, we applied eDNA technology to the six non-native cyprinid species putatively present in a 2.6 river mile stretch of the Chicago (IL, USA) canal system that was subsequently treated with piscicide. The proportion of water samples yielding positive detections increased with relative abundance of the six species, as indicated by the number of carcasses recovered after poisoning. New markers for black carp, grass carp, and a common carp/goldfish are reported and details of the marker testing to ensure specificity are provided.

Cooperative binding mitigates the high-dose hook effect.

PubMed

Roy, Ranjita Dutta; Rosenmund, Christian; Stefan, Melanie I

2017-08-14

The high-dose hook effect (also called prozone effect) refers to the observation that if a multivalent protein acts as a linker between two parts of a protein complex, then increasing the amount of linker protein in the mixture does not always increase the amount of fully formed complex. On the contrary, at a high enough concentration range the amount of fully formed complex actually decreases. It has been observed that allosterically regulated proteins seem less susceptible to this effect. The aim of this study was two-fold: First, to investigate the mathematical basis of how allostery mitigates the prozone effect. And second, to explore the consequences of allostery and the high-dose hook effect using the example of calmodulin, a calcium-sensing protein that regulates the switch between long-term potentiation and long-term depression in neurons. We use a combinatorial model of a "perfect linker protein" (with infinite binding affinity) to mathematically describe the hook effect and its behaviour under allosteric conditions. We show that allosteric regulation does indeed mitigate the high-dose hook effect. We then turn to calmodulin as a real-life example of an allosteric protein. Using kinetic simulations, we show that calmodulin is indeed subject to a hook effect. We also show that this effect is stronger in the presence of the allosteric activator Ca 2+ /calmodulin-dependent kinase II (CaMKII), because it reduces the overall cooperativity of the calcium-calmodulin system. It follows that, surprisingly, there are conditions where increased amounts of allosteric activator actually decrease the activity of a protein. We show that cooperative binding can indeed act as a protective mechanism against the hook effect. This will have implications in vivo where the extent of cooperativity of a protein can be modulated, for instance, by allosteric activators or inhibitors. This can result in counterintuitive effects of decreased activity with increased concentrations of

A refined electrofishing technique for collecting Silver Carp: Implications for management

USGS Publications Warehouse

Bouska, Wesley W.; Glover, David C.; Bouska, Kristen; Garvey, James E.

2017-01-01

Detecting nuisance species at low abundance or in newly established areas is critical to developing pest management strategies. Due to their sensitivity to disturbance and erratic jumping behavior, Silver Carp Hypophthalmichthys molitrix can be difficult to collect with traditional sampling methods. We compared catch per unit effort (CPUE) of all species from a Long Term Resource Monitoring (LTRM) electrofishing protocol to an experimental electrofishing technique designed to minimize Silver Carp evasion through tactical boat maneuvering and selective application of power. Differences in CPUE between electrofishing methods were detected for 2 of 41 species collected across 2 years of sampling at 20 sites along the Illinois River. The mean catch rate of Silver Carp using the experimental technique was 2.2 times the mean catch rate of the LTRM electrofishing technique; the increased capture efficiency at low relative abundance emphasizes the utility of this method for early detection. The experimental electrofishing also collected slightly larger Silver Carp (mean: 510.7 mm TL versus 495.2 mm TL), and nearly four times as many Silver Carp independently jumped into the boat during experimental transects. Novel sampling approaches, such as the experimental electrofishing technique used in this study, should be considered to increase probability of detection for aquatic nuisance species.

Effects of toxic cyanobacteria and ammonia on flesh quality of blunt snout bream (Megalobrama amblycephala).

PubMed

Wang, Li; Chen, Chuanyue; Liu, Wanjing; Xia, Hu; Li, Jian; Zhang, Xuezhen

2017-03-01

Toxic cyanobacterial blooms result in the production of an organic biomass containing cyanotoxins (e.g. microcystins) and an elevated ammonia concentration in the water environment. The ingestion of toxic cyanobacteria and exposure to ammonia are grave hazards for fish. The present study assessed the effects of dietary toxic cyanobacteria and ammonia exposure on the flesh quality of blunt snout bream (Megalobrama amblycephala). Dietary toxic cyanobacteria and ammonia exposure had no impact on fish growth performance, fillet proximate composition and drip loss, whereas it significantly decreased fillet total amino acids, total essential amino acids, hardness and gumminess, and increased fillet ultimate pH as well as malondialdehyde content. However, there was no significant interaction between dietary toxic cyanobacteria and ammonia exposure on these parameters. Additionally, dietary toxic cyanobacteria significantly increased fillet initial pH, thaw loss and protein carbonyl content, whereas ammonia exposure did not. The results of the present study indicate that dietary toxic cyanobacteria and ammonia exposure reduced the quality of blunt snout bream fillet. © 2016 Society of Chemical Industry. © 2016 Society of Chemical Industry.

Role of PIN-mediated auxin efflux in apical hook development of Arabidopsis thaliana.

PubMed

Zádníková, Petra; Petrásek, Jan; Marhavy, Peter; Raz, Vered; Vandenbussche, Filip; Ding, Zhaojun; Schwarzerová, Katerina; Morita, Miyo T; Tasaka, Masao; Hejátko, Jan; Van Der Straeten, Dominique; Friml, Jirí; Benková, Eva

2010-02-01

The apical hook of dark-grown Arabidopsis seedlings is a simple structure that develops soon after germination to protect the meristem tissues during emergence through the soil and that opens upon exposure to light. Differential growth at the apical hook proceeds in three sequential steps that are regulated by multiple hormones, principally auxin and ethylene. We show that the progress of the apical hook through these developmental phases depends on the dynamic, asymmetric distribution of auxin, which is regulated by auxin efflux carriers of the PIN family. Several PIN proteins exhibited specific, partially overlapping spatial and temporal expression patterns, and their subcellular localization suggested auxin fluxes during hook development. Genetic manipulation of individual PIN activities interfered with different stages of hook development, implying that specific combinations of PIN genes are required for progress of the apical hook through the developmental phases. Furthermore, ethylene might modulate apical hook development by prolonging the formation phase and strongly suppressing the maintenance phase. This ethylene effect is in part mediated by regulation of PIN-dependent auxin efflux and auxin signaling.

Blunt Snout Bream (Megalobrama amblycephala) MyD88 and TRAF6: Characterisation, Comparative Homology Modelling and Expression

PubMed Central

Tran, Ngoc Tuan; Liu, Han; Jakovlić, Ivan; Wang, Wei-Min

2015-01-01

MyD88 and TRAF6 play an essential role in the innate immune response in most animals. This study reports the full-length MaMyD88 and MaTRAF6 genes identified from the blunt snout bream (Megalobrama amblycephala) transcriptome profile. MaMyD88 is 2501 base pairs (bp) long, encoding a putative protein of 284 amino acids (aa), including the N-terminal DEATH domain of 78 aa and the C-terminal TIR domain of 138 aa. MaTRAF6 is 2252 bp long, encoding a putative protein of 542 aa, including the N-terminal low-complexity region, RING domain (40 aa), a coiled-coil region (64 aa) and C-terminal MATH domain (147 aa). Coding regions of MaMyD88 and MaTRAF6 genomic sequences consisted of five and six exons, respectively. Physicochemical and functional characteristics of the proteins were analysed. Alpha helices were dominant in the secondary structure of the proteins. Homology models of the MaMyD88 and MaTRAF6 domains were constructed applying the comparative modelling method. RT-qPCR was used to analyse the expression of MaMyD88 and MaTRAF6 mRNA transcripts in response to Aeromonas hydrophila challenge. Both genes were highly upregulated in the liver, spleen and kidney during the first 24 h after the challenge. While MyD88 and TRAF6 have been reported in various aquatic species, this is the first report and characterisation of these genes in blunt snout bream. This research also provides evidence of the important roles of these two genes in the blunt snout bream innate immune system. PMID:25830478

How could fully scaled carps appear in natural waters in Madagascar?

PubMed Central

Hervet, Caroline

2016-01-01

The capacity of organisms to rapidly evolve in response to environmental changes is a key feature of evolution, and studying mutation compensation is a way to evaluate whether alternative routes of evolution are possible or not. Common carps (Cyprinus carpio) carrying a homozygous loss-of-function mutation for the scale cover gene fgfr1a1, causing the ‘mirror’ reduced scale cover, were introduced in Madagascar a century ago. Here we show that carps in Malagasy natural waters are now predominantly covered with scales, though they still all carry the homozygous mutation. We also reveal that the number of scales in mutated carps is under strong polygenic genetic control, with a heritability of 0.49. As a whole, our results suggest that carps submitted to natural selection could evolve a wild-type-like scale cover in less than 40 generations from standing polygenic genetic variation, confirming similar findings mainly retrieved from model organisms. PMID:27559059

How could fully scaled carps appear in natural waters in Madagascar?

PubMed

Hubert, Jean-Noël; Allal, François; Hervet, Caroline; Ravakarivelo, Monique; Jeney, Zsigmond; Vergnet, Alain; Guyomard, René; Vandeputte, Marc

2016-08-31

The capacity of organisms to rapidly evolve in response to environmental changes is a key feature of evolution, and studying mutation compensation is a way to evaluate whether alternative routes of evolution are possible or not. Common carps (Cyprinus carpio) carrying a homozygous loss-of-function mutation for the scale cover gene fgfr1a1, causing the 'mirror' reduced scale cover, were introduced in Madagascar a century ago. Here we show that carps in Malagasy natural waters are now predominantly covered with scales, though they still all carry the homozygous mutation. We also reveal that the number of scales in mutated carps is under strong polygenic genetic control, with a heritability of 0.49. As a whole, our results suggest that carps submitted to natural selection could evolve a wild-type-like scale cover in less than 40 generations from standing polygenic genetic variation, confirming similar findings mainly retrieved from model organisms. © 2016 The Author(s).

Sandy Hook : alternative access concept plan and vehicle replacement study

DOT National Transportation Integrated Search

2009-06-01

This study addresses two critical issues of concern to the Sandy Hook Unit of Gateway National : Recreational Area: (1) options for alternative access to Sandy Hook during peak summer season, : particularly when the park is closed to private vehicles...

The effects of flagellar hook compliance on motility of monotrichous bacteria: A modeling study

NASA Astrophysics Data System (ADS)

Shum, H.; Gaffney, E. A.

2012-06-01

A crucial structure in the motility of flagellated bacteria is the hook, which connects the flagellum filament to the motor in the cell body. Early mathematical models of swimming bacteria assume that the helically shaped flagellum rotates rigidly about its axis, which coincides with the axis of the cell body. Motivated by evidence that the hook is much more flexible than the rest of the flagellum, we develop a new model that allows a naturally straight hook to bend. Hook dynamics are based on the Kirchhoff rod model, which is combined with a boundary element method for solving viscous interactions between the bacterium and the surrounding fluid. For swimming in unbounded fluid, we find good support for using a rigid model since the hook reaches an equilibrium configuration within several revolutions of the motor. However, for effective swimming, there are constraints on the hook stiffness relative to the scale set by the product of the motor torque with the hook length. When the hook is too flexible, its shape cannot be maintained and large deformations and stresses build up. When the hook is too rigid, the flagellum does not align with the cell body axis and the cell "wobbles" with little net forward motion. We also examine the attraction of swimmers to no-slip surfaces and find that the tendency to swim steadily close to a surface can be very sensitive to the combination of the hook rigidity and the precise shape of the cell and flagellum.

Characterization of complete genome sequence of the spring viremia of carp virus isolated from common carp (Cyprinus carpio) in China.

PubMed

Teng, Y; Liu, H; Lv, J Q; Fan, W H; Zhang, Q Y; Qin, Q W

2007-01-01

The complete genome of spring viraemia of carp virus (SVCV) strain A-1 isolated from cultured common carp (Cyprinus carpio) in China was sequenced and characterized. Reverse transcription-polymerase chain reaction (RT-PCR) derived clones were constructed and the DNA was sequenced. It showed that the entire genome of SVCV A-1 consists of 11,100 nucleotide base pairs, the predicted size of the viral RNA of rhabdoviruses. However, the additional insertions in bp 4633-4676 and bp 4684-4724 of SVCV A-1 were different from the other two published SVCV complete genomes. Five open reading frames (ORFs) of SVCV A-1 were identified and further confirmed by RT-PCR and DNA sequencing of their respective RT-PCR products. The 5 structural proteins encoded by the viral RNA were ordered 3'-N-P-M-G-L-5'. This is the first report of a complete genome sequence of SVCV isolated from cultured carp in China. Phylogenetic analysis indicates that SVCV A-1 is closely related to the members of the genus Vesiculovirus, family Rhabdoviridae.

Development and impact of hooks of high droplet concentration on remote southeast Pacific stratocumulus

NASA Astrophysics Data System (ADS)

George, R. C.; Wood, R.; Bretherton, C. S.; Painter, G.

2013-07-01

Over the southeastern Pacific (SEP), droplet concentration (Nd) in the typically unpolluted marine stratocumulus west of 80° W (> 1000 km offshore) is periodically strongly enhanced in zonally elongated "hook"-shaped features that increase albedo. Here, we examine three hook events using the chemistry version of the Weather Research and Forecasting model (WRF-Chem) with 14 km horizontal resolution, satellite data, and aircraft data from the VAMOS Ocean-Cloud-Atmosphere-Land Study Regional Experiment (VOCALS-REx). A particularly strong hook yields insights into the development, decay, and radiative impact of these features. Hook development occurs with Nd increasing to polluted levels over the remote ocean primarily due to entrainment of cloud condensation nuclei (CCN) from the lower free troposphere (FT). The feature advects northwestward until the FT CCN source is depleted, after which Nd decreases over a few days due to precipitation and dilution. The model suggests that the FT CCN source supplying the hook consists of high concentrations of small accumulation-mode aerosols that contribute a relatively small amount of aerosol mass to the MBL, in agreement with near-coast VOCALS measurements of polluted layers in the FT. The aerosol particles in this hook originate mainly from a pulse of offshore flow that transports Santiago-region (33-35° S) emissions to the remote marine FT. To provide pollution CCN that can sustain hooks, the FT transport of pollution plumes to the remote ocean requires strong, deep offshore flow. Such flow is favored by a trough approaching the South American coast and a southeastward shift of the climatological subtropical high-pressure system. The model simulations show precipitation suppression in the hook and a corresponding increase in liquid water path (LWP) compared with a simulation without anthropogenic sources. LWP also increases as the hook evolves over time due to increasing stability and decreasing subsidence. WRF-Chem suggests

Dimensional threshold for fracture linkage and hooking

NASA Astrophysics Data System (ADS)

Lamarche, Juliette; Chabani, Arezki; Gauthier, Bertrand D. M.

2018-03-01

Fracture connectivity in rocks depends on spatial properties of the pattern including length, abundance and orientation. When fractures form a single-strike set, they hardly cross-cut each other and the connectivity is limited. Linkage probability increases with increasing fracture abundance and length as small fractures connect to each other to form longer ones. A process for parallel fracture linkage is the "hooking", where two converging fracture tips mutually deviate and then converge to connect due to the interaction of their crack-tip stresses. Quantifying the processes and conditions for fracture linkage in single-strike fracture sets is crucial to better predicting fluid flow in Naturally Fractured Reservoirs. For 1734 fractures in Permian shales of the Lodève Basin, SE France, we measured geometrical parameters in 2D, characterizing three stages of the hooking process: underlapping, overlapping and linkage. We deciphered the threshold values, shape ratios and limiting conditions to switch from one stage to another one. The hook set up depends on the spacing (S) and fracture length (Lh) with the relation S ≈ 0.15 Lh. Once the hooking is initiated, with the fracture deviation length (L) L ≈ 0.4 Lh, the fractures reaches the linkage stage only when the spacing is reduced to S ≈ 0.02 Lh and the convergence (C) is < 0.1 L. These conditions apply to multi-scale fractures with a shape ratio L/S = 10 and for fracture curvature of 10°-20°.

Validation of eDNA Surveillance Sensitivity for Detection of Asian Carps in Controlled and Field Experiments

PubMed Central

Mahon, Andrew R.; Jerde, Christopher L.; Galaska, Matthew; Bergner, Jennifer L.; Chadderton, W. Lindsay; Lodge, David M.; Hunter, Margaret E.; Nico, Leo G.

2013-01-01

In many North American rivers, populations of multiple species of non-native cyprinid fishes are present, including black carp (Mylpharyngodon piceus), grass carp (Ctenopharyngodon idella), bighead carp (Hypophthalmichthys nobilis), silver carp (Hypophthalmichthys molitrix), common carp (Cyprinus carpio), and goldfish (Carassius auratus). All six of these species are found in the Mississippi River basin and tracking their invasion has proven difficult, particularly where abundance is low. Knowledge of the location of the invasion front is valuable to natural resource managers because future ecological and economic damages can be most effectively prevented when populations are low. To test the accuracy of environmental DNA (eDNA) as an early indicator of species occurrence and relative abundance, we applied eDNA technology to the six non-native cyprinid species putatively present in a 2.6 river mile stretch of the Chicago (IL, USA) canal system that was subsequently treated with piscicide. The proportion of water samples yielding positive detections increased with relative abundance of the six species, as indicated by the number of carcasses recovered after poisoning. New markers for black carp, grass carp, and a common carp/goldfish are reported and details of the marker testing to ensure specificity are provided. PMID:23472178

Reflections on Sandy Hook

ERIC Educational Resources Information Center

Trump, Kenneth S.

2013-01-01

In this article, the author shares his thoughts for district administrators regarding the Sandy Hook Elementary school tragedy. Administrators heard a lot of potential solutions or attempts at solutions. However, these proposals raise lengthy lists of implementation questions and issues that illustrate a lack of understanding of school operations,…

Protein relaxation without a geminate phase in nanosecond photodissociated CO carp hemoglobin

NASA Astrophysics Data System (ADS)

Loupiac, Camille; Kruk, Nicolay; Valat, Pierre; Alpert, Bernard

1999-03-01

Transient heme-protein interactions upon passing from ligated to deligated carp hemoglobin were observed through time-resolved optical spectra following nanosecond CO photodissociation. The spectral evolution of the heme, in the nanosecond and microsecond time ranges, shows a protein conformational relaxation and the absence of a geminate CO recombination in carp hemoglobin. The comparison of the phenomena in carp and human hemoglobin implies that the physical basis of the geminate rebinding in human hemoglobin should involve an out-of-equilibrium protein conformation, close to a dissipative structure defined by the thermodynamics of Prigogine.

Bohlin transformation: the hidden symmetry that connects Hooke to Newton

NASA Astrophysics Data System (ADS)

Saggio, Maria Luisa

2013-01-01

Hooke's name is familiar to students of mechanics thanks to the law of force that bears his name. Less well-known is the influence his findings had on the founder of mechanics, Isaac Newton. In a lecture given some twenty years ago, W Arnol'd pointed out the outstanding contribution to science made by Hooke, and also noted the controversial issue of the attribution of important discoveries to Newton that were actually inspired by Hooke. It therefore seems ironic that the two most famous force laws, named after Hooke and Newton, are two geometrical aspects of the same law. This relationship, together with other illuminating aspects of Newtonian mechanics, is described in Arnol'd's book and is worth remembering in standard physics courses. In this didactical paper the duality of the two forces is expounded and an account of the more recent contributions to the subject is given.

Absorption of metals in mulloway (Argyrosomus japonicus) after ingesting nickel-plated carbon-steel hooks.

PubMed

McGrath, Shane P; Reichelt-Brushett, Amanda J; Butcher, Paul A; Cairns, Stuart C

2014-08-01

Previous research has alluded to the potential of metals being absorbed by fish after ingesting fishing hooks, which may have adverse effects on fish health and the organisms that consume them. Subsequently, this study aimed to quantify the potential of mulloway (Argyrosomus japonicus) to absorb metals during the decay of ingested nickel-plated carbon-steel hooks. Twenty-five treatment fish were allowed to ingest nickel-plated carbon-steel hooks during angling and then monitored with 25 controls (untreated fish) for up to 42 days for hook ejection and mortality. Blood, liver and muscle samples were collected from treatment, control and 14 wild-caught individuals to determine the concentrations of chromium, cobalt, copper, iron, manganese and nickel. The results showed that increased oxidation influenced hook ejection, and that hook-ingested fish had significantly elevated concentrations of nickel in their liver and blood, but not muscle. This research has shown that there is an avenue for metal absorption from ingested hooks. Copyright © 2014 Elsevier Ltd. All rights reserved.

Feasibility of an implantable capsule for limiting lifespan of grass carp

USGS Publications Warehouse

Thomas, R.M.; Miranda, L.E.; Kirk, J.P.

2006-01-01

The grass carp (Ctenopharyngodon idella) is an herbivorous cyprinid stocked to control undesirable aquatic vegetation. However, stocking grass carp presents several problems including complete eradication of submersed aquatic vegetation, dispersal out of the target area, adverse effects on fish communities, and damage to waterfowl habitat and native vegetation. The purpose of this research was to consider the feasibility of an implantable capsule for limiting the lifespan of grass carp. Stainless steel dowel pins were inserted into 49 fish to identify the most appropriate site to implant the capsule. The throat region along the body's longitudinal axis was identified as the most suitable location because it resulted in minimal loss over an 8-month holding period. Rotenone solutions were injected into the ventral surface between the pelvic fins to determine the lethal dosage to 95% of the population (LD 95). The LD95 for grass carp increased curvilin-early with fish weight. Four polymers that merit further evaluation in constructing the capsule are poly[bis(p-carboxyphenoxy) propane anhydride], poly[bis(p- carboxyphenoxy) hexane anhydride], poly-1-lactide, and poly(??-caprolactone) . Implants are commonly used to deliver pharmaceutical products in medical and veterinarian applications, and have been used in fish. Developing a bioerodible capsule could increase the safety and flexibility of stocking grass carp for control of aquatic plants, and may also be applicable for management of other exotic species.

Effects of growth hormone over-expression on reproduction in the common carp Cyprinus carpio L.

PubMed

Cao, Mengxi; Chen, Ji; Peng, Wei; Wang, Yaping; Liao, Lanjie; Li, Yongming; Trudeau, Vance L; Zhu, Zuoyan; Hu, Wei

2014-01-01

To study the complex interaction between growth and reproduction we have established lines of transgenic common carp (Cyprinus carpio) carrying a grass carp (Ctenopharyngodon idellus) growth hormone (GH) transgene. The GH-transgenic fish showed delayed gonadal development compared with non-transgenic common carp. To gain a better understanding of the phenomenon, we studied body growth, gonad development, changes of reproduction related genes and hormones of GH-transgenic common carp for 2years. Over-expression of GH elevated peripheral gh transcription, serum GH levels, and inhibited endogenous GH expression in the pituitary. Hormone analyses indicated that GH-transgenic common carp had reduced pituitary and serum level of luteinizing hormone (LH). Among the tested genes, pituitary lhβ was inhibited in GH-transgenic fish. Further analyses in vitro showed that GH inhibited lhβ expression. Localization of ghr with LH indicates the possibility of direct regulation of GH on gonadotrophs. We also found that GH-transgenic common carp had reduced pituitary sensitivity to stimulation by co-treatments with a salmon gonadotropin-releasing hormone (GnRH) agonist and a dopamine antagonist. Together these results suggest that the main cause of delayed reproductive development in GH transgenic common carp is reduced LH production and release. Copyright © 2013 Elsevier Inc. All rights reserved.

Management implications of broadband sound in modulating wild silver carp (Hypophthalmichthys molitrix) behavior

USGS Publications Warehouse

Vetter, Brooke J.; Calfee, Robin D.; Mensinger, Allen F.

2017-01-01

Invasive silver carp (Hypophthalmichthys molitrix) dominate large regions of the Mississippi River drainage, outcompete native species, and are notorious for their prolific and unusual jumping behavior. High densities of juvenile and adult (~25 kg) carp are known to jump up to 3 m above the water surface in response to moving watercraft. Broadband sound recorded from an outboard motor (100 hp at 32 km/hr) can modulate their behavior in captivity; however, the response of wild silver carp to broadband sound has yet to be determined. In this experiment, broadband sound (0.06–10 kHz) elicited jumping behavior from silver carp in the Spoon River near Havana, IL independent of boat movement, indicating acoustic stimulus alone is sufficient to induce jumping. Furthermore, the number of jumping fish decreased with subsequent sound exposures. Understanding silver carp jumping is not only important from a behavioral standpoint, it is also critical to determine effective techniques for controlling this harmful species, such as herding fish into a net for removal.

Sandy Hook Traveler Information System

DOT National Transportation Integrated Search

2010-09-01

This report focuses on equipment and procedural solutions for gathering and disseminating a wide range of visitor information, including real-time traveler information data relating to traffic and parking at the Sandy Hook Unit of the Gateway Recreat...

Comparative analysis of the intestinal bacterial communities in different species of carp by pyrosequencing.

PubMed

Li, Tongtong; Long, Meng; Gatesoupe, François-Joël; Zhang, Qianqian; Li, Aihua; Gong, Xiaoning

2015-01-01

Gut microbiota is increasingly regarded as an integral component of the host, due to important roles in the modulation of the immune system, the proliferation of the intestinal epithelium and the regulation of the dietary energy intake. Understanding the factors that influence the composition of these microbial communities is essential to health management, and the application to aquatic animals still requires basic investigation. In this study, we compared the bacterial communities harboured in the intestines and in the rearing water of grass carp (Ctenopharyngodon idellus), crucian carp (Carassius cuvieri), and bighead carp (Hypophthalmichthys nobilis), by using 454-pyrosequencing with barcoded primers targeting the V4 to V5 regions of the bacterial 16S rRNA gene. The specimens of the three species were cohabiting in the same pond. Between 6,218 and 10,220 effective sequences were read from each sample, resulting in a total of 110,398 sequences for 13 samples from gut microbiota and pond water. In general, the microbial communities of the three carps were dominated by Fusobacteria, Firmicutes, Proteobacteria and Bacteroidetes, but the abundance of each phylum was significantly different between species. At the genus level, the overwhelming group was Cetobacterium (97.29 ± 0.46 %) in crucian carp, while its abundance averaged c. 40 and 60 % of the sequences read in the other two species. There was higher microbial diversity in the gut of filter-feeding bighead carp than the gut of the two other species, with grazing feeding habits. The composition of intestine microbiota of grass carp and crucian carp shared higher similarity when compared with bighead carp. The principal coordinates analysis (PCoA) with the weighted UniFrac distance and the heatmap analysis suggested that gut microbiota was not a simple reflection of the microbial community in the local habitat but resulted from species-specific selective pressures, possibly dependent on behavioural, immune

Emergence of carp edema virus in cultured ornamental koi carp, Cyprinus carpio koi, in India.

PubMed

Swaminathan, T Raja; Kumar, Raj; Dharmaratnam, Arathi; Basheer, V S; Sood, Neeraj; Pradhan, P K; Sanil, N K; Vijayagopal, P; Jena, J K

2016-12-01

A disease outbreak was reported in adult koi, Cyprinus carpio koi, from a fish farm in Kerala, India, during June 2015. The clinical signs were observed only in recently introduced adult koi, and an existing population of fish did not show any clinical signs or mortality. Microscopic examination of wet mounts from the gills of affected koi revealed minor infestation of Dactylogyrus sp. in a few koi. In bacteriological studies, only opportunistic bacteria were isolated from the gills of affected fish. The histopathological examination of the affected fish revealed necrotic changes in gills and, importantly, virus particles were demonstrated in cytoplasm of gill epithelial cells in transmission electron microscopy. The tissue samples from affected koi were negative for common viruses reported from koi viz. cyprinid herpesvirus 3, spring viraemia of carp virus, koi ranavirus and red sea bream iridovirus in PCR screening. However, gill tissue from affected koi carp was positive for carp edema virus (CEV) in the first step of nested PCR, and sequencing of PCR amplicons confirmed infection with CEV. No cytopathic effect was observed in six fish cell lines following inoculation of filtered tissue homogenate prepared from gills of affected fish. In bioassay, the symptoms could be reproduced by inoculation of naive koi with filtrate from gill tissue homogenate of CEV-positive fish. Subsequently, screening of koi showing clinical signs similar to koi sleepy disease from different locations revealed that CEV infection was widespread. To our knowledge, this is the first report of infection with CEV in koi from India.

The hook effect in calcitonin immunoradiometric assay: a case report.

PubMed

Fangous, Marie-Sarah; Kerspern, Hélène; Moineau, Marie-Pierre; Kerlan, Véronique; Alavi, Zarrin; Carré, Jean-Luc

2012-12-01

The hook effect, which has long been detected and documented for immunoradiometric assays (IRMA) such as those measuring prolactin or thyroglobulin, occurs when the serum antigen level is extremely high, thus inducing a bias in the methodology of measurement. We report the case of an 80-year-old man with confirmed medullary thyroid carcinoma (MTC). In the case reported here, the clinical status of the patient contrasts with his tumor antigen, serum calcitonin (CT), concentrations. The measured increased CT concentrations revealed the presence of a hook effect. This phenomenon occurs due to an excess of antigen during the one-step IRMA where the signal antibodies, bound to the non-captured antigens, are washed out during the measurement, inducing the loss of signal. Aiming to prevent the "hook effect", successive dilutions of the same sample of serum were done. Previous studies have shown when one-step IRMA reveals high concentrations of a tumor serum antigen (i.e. prolactin or thyroglobulin), a two-step IRMA or a systematic 1:10 dilution of the serum sample prevents the formation of the "hook effect". In our case report, the CT "hook effect" formation was prevented by performing serial dilutions of the serum sample. Copyright © 2012 Elsevier Masson SAS. All rights reserved.

Age, growth, and gonadal characteristics of adult bighead carp, Hypophthalmichthys nobilis, in the lower Missouri River

USGS Publications Warehouse

Schrank, S.J.; Guy, C.S.

2002-01-01

Bighead carp were introduced into Arkansas in 1973 to improve water clarity in production ponds. Bighead carp subsequently escaped aquaculture facilities in the early 1980's and dispersed into the Mississippi and Missouri rivers. The first documentation of bighead carp reproduction in the Mississippi River system was in 1989. The population has increased in the Missouri River as is evident in their increased proportion in the commercial harvest since 1990. The effect of this exotic planktivore on native ecosystems of the U.S. has not been examined. Basic biological data on bighead carp Hypophthalmichthys nobilis in the Missouri River are needed to predict potential ecological problems and provide a foundation for manipulative studies. The objectives of this study were to assess age, growth, and gonadal characteristics of bighead carp in the Missouri River. Adult bighead carp in our sample varied from age 3 to age 7 and length varied from 475 to 1050 mm. There was a large variation in length at age, and overall bighead carp exhibited fast growth. For example, mean back-calculated length at age 3 was 556 mm. The sample was dominated by bighead carp from the 1994 year class. There was no difference in gonad development (i.e., gonadal somatic index, egg diameter) between winter and spring samples. Length of male bighead carp and GSI were not significantly correlated; however, females exhibited a positive linear relationship between length and GSI. In each ovary, egg diameter frequencies exhibited a bimodal distribution, indicating protracted spawning. Mean fecundity was 226 213, with a maximum fecundity of 769 964. Bighead carp in the Missouri River have similar life history characteristics to Asian and European populations. They have become well established in the Missouri River and it is likely that dispersal and population density will increase.

Variations in 5S rDNAs in diploid and tetraploid offspring of red crucian carp × common carp.

PubMed

Ye, Lihai; Zhang, Chun; Tang, Xiaojun; Chen, Yiyi; Liu, Shaojun

2017-08-08

The allotetraploid hybrid fish (4nAT) that was created in a previous study through an intergeneric cross between red crucian carp (Carassius auratus red var., ♀) and common carp (Cyprinus carpio L., ♂) provided an excellent platform to investigate the effect of hybridization and polyploidization on the evolution of 5S rDNA. The 5S rDNAs of paternal common carp were made up of a coding sequence (CDS) and a non-transcribed spacer (NTS) unit, and while the 5S rDNAs of maternal red crucian carp contained a CDS and a NTS unit, they also contained a variable number of interposed regions (IPRs). The CDSs of the 5S rDNAs in both parental fishes were conserved, while their NTS units seemed to have been subjected to rapid evolution. The diploid hybrid 2nF 1 inherited all the types of 5S rDNAs in both progenitors and there were no signs of homeologous recombination in the 5S rDNAs of 2nF 1 by sequencing of PCR products. We obtained two segments of 5S rDNA with a total length of 16,457 bp from allotetraploid offspring 4nAT through bacterial artificial chromosome (BAC) sequencing. Using this sequence together with the 5S rDNA sequences amplified from the genomic DNA of 4nAT, we deduced that the 5S rDNAs of 4nAT might be inherited from the maternal progenitor red crucian carp. Additionally, the IPRs in the 5S rDNAs of 4nAT contained A-repeats and TA-repeats, which was not the case for the IPRs in the 5S rDNAs of 2nF 1 . We also detected two signals of a 200-bp fragment of 5S rDNA in the chromosomes of parental progenitors and hybrid progenies by fluorescence in situ hybridization (FISH). We deduced that during the evolution of 5S rDNAs in different ploidy hybrid fishes, interlocus gene conversion events and tandem repeat insertion events might occurred in the process of polyploidization. This study provided new insights into the relationship among the evolution of 5S rDNAs, hybridization and polyploidization, which were significant in clarifying the genome evolution of

Development and impact of hooks of large droplet concentration on remote southeast Pacific stratocumulus

NASA Astrophysics Data System (ADS)

George, R. C.; Wood, R.; Bretherton, C. S.; Painter, G.

2013-01-01

Over the southeastern Pacific (SEP), droplet concentration (Nd) in the typically unpolluted marine stratocumulus west of 80° W (> 1000 km offshore) is periodically strongly enhanced in zonally-elongated "hook"-shaped arcs that increase albedo. Here, we examine three hook events using the chemistry version of the Weather Research and Forecasting model (WRF-Chem) with 14 km horizontal resolution, satellite data and aircraft data from the VAMOS Ocean-Cloud-Atmosphere-Land Study Regional Experiment (VOCALS-REx). A particularly strong hook yields insights to the development, decay, and radiative impact of these features. Hook development occurs with Nd increasing to polluted levels over the remote ocean primarily due to entrainment of cloud condensation nuclei (CCN) from the free troposphere (FT). The feature advects northwestward until the FT CCN source is depleted, after which Nd decreases over a few days due to precipitation and dilution. The model suggests that the FT CCN source supplying the hook consists of high concentrations of small accumulation mode aerosols that contribute a relatively small amount of aerosol mass to the MBL. The aerosol particles originate mainly from a pulse of offshore flow that transports Santiago region (33-35° S) emissions to the marine FT. To provide a sustained hook CCN source, the FT transport of pollution plumes to the remote ocean requires strong, deep offshore flow. Such flow is favored by a trough approaching the South American coast and a southeastward shift of the climatological subtropical high pressure system. The model simulations show precipitation suppression in the hook and a corresponding increase in liquid water path (LWP) compared with a simulation without anthropogenic sources. LWP also increases in time as the hook evolves due to increasing stability and decreasing subsidence. WRF-Chem suggests that DMS significantly influences the aerosol number and size distributions in a hook, but that hooks do not form without

Comparison of the Exomes of Common Carp (Cyprinus carpio) and Zebrafish (Danio rerio)

PubMed Central

Henkel, Christiaan V.; Dirks, Ron P.; Jansen, Hans J.; Forlenza, Maria; Wiegertjes, Geert F.; Howe, Kerstin; van den Thillart, Guido E.E.J.M.

2012-01-01

Abstract Research on common carp, Cyprinus carpio, is beneficial for zebrafish research because of resources available owing to its large body size, such as the availability of sufficient organ material for transcriptomics, proteomics, and metabolomics. Here we describe the shot gun sequencing of a clonal double-haploid common carp line. The assembly consists of 511891 scaffolds with an N50 of 17 kb, predicting a total genome size of 1.4–1.5 Gb. A detailed analysis of the ten largest scaffolds indicates that the carp genome has a considerably lower repeat coverage than zebrafish, whilst the average intron size is significantly smaller, making it comparable to the fugu genome. The quality of the scaffolding was confirmed by comparisons with RNA deep sequencing data sets and a manual analysis for synteny with the zebrafish, especially the Hox gene clusters. In the ten largest scaffolds analyzed, the synteny of genes is almost complete. Comparisons of predicted exons of common carp with those of the zebrafish revealed only few genes specific for either zebrafish or carp, most of these being of unknown function. This supports the hypothesis of an additional genome duplication event in the carp evolutionary history, which—due to a higher degree of compactness—did not result in a genome larger than that of zebrafish. PMID:22715948

Evidence of Asian carp spawning upstream of a key choke point in the Mississippi River

USGS Publications Warehouse

Larson, James H.; Knights, Brent C.; McCalla, S. Grace; Monroe, Emy; Tuttle-Lau, Maren T.; Chapman, Duane C.; George, Amy E.; Vallazza, Jon; Amberg, Jon J.

2017-01-01

Bighead Carp Hypophthalmichthys nobilis, Silver Carp H. molitrix, and Grass Carp Ctenopharyngodon idella(collectively termed “Asian carp”) were introduced into North America during the 1960s and 1970s and have become established in the lower Mississippi River basin. Previously published evidence for spawning of these species in the upper Mississippi River has been limited to an area just downstream of Dam 22 (near Saverton, Missouri). In 2013 and 2014, we sampled ichthyoplankton at 18 locations in the upper Mississippi River main stem from Dam 9 through Dam 19 and in four tributaries of the Mississippi River (Des Moines, Skunk, Iowa, and Wisconsin rivers). We identified eggs and larvae by using morphological techniques and then used genetic tools to confirm species identity. The spawning events we observed often included more than one species of Asian carp and in a few cases included eggs that must have been derived from more than one upstream spawning event. The upstream extent of genetically confirmed Grass Carp ichthyoplankton was the Wisconsin River, while Bighead Carp and Silver Carp ichthyoplankton were observed in Pool 16. In all these cases, ichthyoplankton likely drifted downstream for several hours prior to collection. Higher water velocities (and, to a lesser extent, higher temperatures) were associated with an increased likelihood of observing eggs or larvae, although the temperature range we encountered was mostly above 17°C. Several major spawning events were detected in 2013, but no major spawning events were observed in 2014. The area between Dam 15 and Dam 19 appears to be the upstream edge of spawning activity for both Silver Carp and Bighead Carp, suggesting that this area could be a focal point for management efforts designed to limit further upstream movement of these species..

Complexity of gap junctions between horizontal cells of the carp retina.

PubMed

Greb, H; Hermann, S; Dirks, P; Ommen, G; Kretschmer, V; Schultz, K; Zoidl, G; Weiler, R; Janssen-Bienhold, U

2017-01-06

In the vertebrate retina, horizontal cells (HCs) reveal homologous coupling by gap junctions (gj), which are thought to consist of different connexins (Cx). However, recent studies in mouse, rabbit and zebrafish retina indicate that individual HCs express more than one connexin. To provide further insights into the composition of gj connecting HCs and to determine whether HCs express multiple connexins, we examined the molecular identity and distribution of gj between HCs of the carp retina. We have cloned four carp connexins designated Cx49.5, Cx55.5, Cx52.6 and Cx53.8 with a close relationship to connexins previously reported in HCs of mouse, rabbit and zebrafish, respectively. Using in situ hybridization, Cx49.5 expression was detected in different subpopulations of retinal neurons including HCs, whereas the Cx52.6 transcript was localized exclusively in HCs. Using specific antibodies, Cx55.5 and Cx53.8 were detected on dendrites of all four HC subtypes and axon terminals. Immunoelectron microscopy confirmed the presence of Cx55.5 and Cx53.8 in gap junctions between these processes and Cx55.5 was additionally observed in HC dendrites invaginating cone pedicles, suggesting its participation in the modulation of photoreceptor output in the carp retina. Furthermore, using single-cell RT-PCR, all four connexins were detected in different subtypes of HCs, suggesting overlapping expression patterns. Thus, the composition of gj mediating homologous coupling between subtypes of carp HCs appears to be more complex than expected. Moreover, BLAST searches of the preliminary carp genome, using novel sequences as query, suggest that most of the analyzed connexin genes are duplicated in carp. Crown Copyright © 2016. Published by Elsevier Ltd. All rights reserved.

Piezoelectricity of green carp scales

NASA Astrophysics Data System (ADS)

Jiang, H. Y.; Yen, F.; Huang, C. W.; Mei, R. B.; Chen, L.

2017-04-01

Piezoelectricity takes part in multiple important functions and processes in biomaterials often vital to the survival of organisms. Here, we investigate the piezoelectric properties of fish scales of green carp by directly examining their morphology at nanometer levels. Two types of regions are found to comprise the scales, a smooth one and a rough one. The smooth region is comprised of a ridge and trough pattern and the rough region characterized by a flat base with an elevated mosaic of crescents. Piezoelectricity is found on the ridges and base regions of the scales. From clear distinctions between the composition of the inner and outer surfaces of the scales, we identify the piezoelectricity to originate from the presence of hydroxyapatite which only exists on the surface of the fish scales. Our findings reveal a different mechanism of how green carp are sensitive to their surroundings and should be helpful to studies related to the electromechanical properties of marine life and the development of bio-inspired materials.

Silver, bighead, and common carp orient to acoustic particle motion when avoiding a complex sound.

PubMed

Zielinski, Daniel P; Sorensen, Peter W

2017-01-01

Behavioral responses of silver carp (Hypopthalmichthys molitrix), bighead carp (H. nobilis), and common carp (Cyprinus carpio) to a complex, broadband sound were tested in the absence of visual cues to determine whether these species are negatively phonotaxic and the roles that sound pressure and particle motion might play mediating this response. In a dark featureless square enclosure, groups of 3 fish were tracked and the distance of each fish from speakers and their swimming trajectories relative to sound pressure and particle acceleration were analyzed before, and then while an outboard motor sound was played. All three species exhibited negative phonotaxis during the first two exposures after which they ceased responding. The median percent time fish spent near the active speaker for the first two trials decreased from 7.0% to 1.3% for silver carp, 7.9% to 1.1% for bighead carp, and 9.5% to 3% for common carp. Notably, when close to the active speaker fish swam away from the source and maintained a nearly perfect 0° orientation to the axes of particle acceleration. Fish did not enter sound fields greater than 140 dB (ref. 1 μPa). These results demonstrate that carp avoid complex sounds in darkness and while initial responses may be informed by sound pressure, sustained oriented avoidance behavior is likely mediated by particle motion. This understanding of how invasive carp use particle motion to guide avoidance could be used to design new acoustic deterrents to divert them in dark, turbid river waters.

Silver, bighead, and common carp orient to acoustic particle motion when avoiding a complex sound

PubMed Central

Sorensen, Peter W.

2017-01-01

Behavioral responses of silver carp (Hypopthalmichthys molitrix), bighead carp (H. nobilis), and common carp (Cyprinus carpio) to a complex, broadband sound were tested in the absence of visual cues to determine whether these species are negatively phonotaxic and the roles that sound pressure and particle motion might play mediating this response. In a dark featureless square enclosure, groups of 3 fish were tracked and the distance of each fish from speakers and their swimming trajectories relative to sound pressure and particle acceleration were analyzed before, and then while an outboard motor sound was played. All three species exhibited negative phonotaxis during the first two exposures after which they ceased responding. The median percent time fish spent near the active speaker for the first two trials decreased from 7.0% to 1.3% for silver carp, 7.9% to 1.1% for bighead carp, and 9.5% to 3% for common carp. Notably, when close to the active speaker fish swam away from the source and maintained a nearly perfect 0° orientation to the axes of particle acceleration. Fish did not enter sound fields greater than 140 dB (ref. 1 μPa). These results demonstrate that carp avoid complex sounds in darkness and while initial responses may be informed by sound pressure, sustained oriented avoidance behavior is likely mediated by particle motion. This understanding of how invasive carp use particle motion to guide avoidance could be used to design new acoustic deterrents to divert them in dark, turbid river waters. PMID:28654676

[ZHENG's gold hook fishing acupuncture for lumbar disc herniation: a clinical observation].

PubMed

Zhu, Bowen; Zhang, Xinghua; Sun, Runjie; Qin, Xiaoguang

2016-04-01

To compare the clinical efficacy differences between Zheng's gold hook, fishing acupuncture and electroacupuncture (EA) for lumbar disc herniation (LDH). Sixty patients of LDH were randomly allocated to a gold hook fishing acupuncture group and an EA group, 30 cases in each one. Lumbar Jiaji (EX-1 B 2), Yaoyangguan (GV 3), Shenshu (BL 23), Dachangshu (BL 25), Guanyuanshu (BL 26) and ashi points were selected in the gold hook fishing acupuncture group; after the needles were inserted, the manipulation of gold hook fishing acupuncture was applied at tendon junction points and ashi points. The identical acupoints were selected in the EA group and patients were treated with EA. The treatment was both given once a day; ten days of treatment were taken as one session, and totally 3 sessions were given. The clinical effective rate, visual analogue scale (VAS), low back pain score and Oswestry disability index (ODI) were used for efficacy evaluation. The effective rate was 93.3% (28/30) in the gold hook fishing acupuncture group, which was superior to 86.7% (26/30) in the EA group (P < 0.05). The VAS, low back pain score and ODI were both significantly improved after treatment (all P < 0.05), which were more significant in the gold hook fishing acupuncture group (all P < 0.05). ZHENG's gold hook fishing acupuncture could effectively improve the symptoms and sings of LDH, reduce the disability index and improve the quality of life, which is superior to EA.

The quality of carp (Cyprinus carpio L.) cultured in various Polish regions.

PubMed

Tkaczewska, Joanna; Migdał, Władysław; Kulawik, Piotr

2014-11-01

Although environmental factors greatly affect the quality of carp meat, no regulations impose labelling the place of cultivation of freshwater fish. That is why the purpose of this study was to determine the quality of carp meat cultivated in several regions of Poland as well as to assess the necessity of implementing such regulations. The influence of the cultivation region on colour, nutrition value, health safety and sensory quality of carp meat was assessed. The analysis included colour measurement, determination of fatty acid profile and sensory evaluation of carp muscle. Moreover, microbiological analysis of the fish surface was performed. The results show that the place of cultivation does not influence the lightness (L*) of fillet, but has an impact on other colour parameters (a*, b*). The microbiological quality of fish from all studied farms was satisfactory, since no pathogenic microorganisms were observed on the fish surface. The fatty acid profile was fairly varied (P < 0.01), depending on the place of cultivation. Since final results seem ambiguous, further analyses of quality features of carp from various regions and cultures should be performed, before any recommendation for the necessity of labelling the place and method of cultivation should be suggested. © 2014 Society of Chemical Industry.

Feeding habits of the deep-snouted pipefish Syngnathus typhle in a temperate coastal lagoon

NASA Astrophysics Data System (ADS)

Oliveira, Frederico; Erzini, Karim; Gonçalves, Jorge M. S.

2007-03-01

Feeding habits of Syngnathus typhle were determined based on monthly sampling during experimental fishing in a coastal lagoon (Ria Formosa, South Portugal) from April 2001 to May 2002. The gut contents of 856 individuals were analysed and quantified with numerical and gravimetric methods, as well as with some complementary indices and measures (vacuity, fullness and relative importance). In this study, this species fed mainly on Copepoda, Mysidacea, small caridean grass shrimps (Hippolytidae and Palaemonidae) and small fishes (Gobiidae) and to a lesser degree on other small invertebrates. No significant differences were found between the diets of males and females. However, significant differences in the fullness indexes of both sexes were observed during the reproductive period. Significant differences in the diet among seasons were also verified but no seasonal trends were discovered. Snout length showed a linear increase with growth while mouth area and total length displayed an allometric relation. Mouth shape varied between an ellipse and an almost perfect circle. The increase in mouth area and snout length as S. typhle grows contributed to the observed ontogenic diet shift with specimens smaller than 10 cm feeding almost exclusively on Copepoda, medium sized specimens feeding mainly on Hippolytidae and Mysidacea, while larger specimens preyed on Hippolytidae, Palaemonidae and Gobiidae. Prey size generally increased with size of S. typhle.

Hydrology controls recruitment of two invasive cyprinids: bigheaded carp reproduction in a navigable large river

PubMed Central

Solomon, Levi E.; Pendleton, Richard M.; Chick, John H.; Casper, Andrew F.

2017-01-01

In the Mississippi River Basin of North America, invasive bigheaded carp (silver carp Hypophthalmichthys molitrix and bighead carp H. nobilis, also referred to as Asian carp) have spread rapidly over the past several decades. In the Illinois River, an important tributary of the Upper Mississippi River, reproduction appears to be sporadic and frequently unsuccessful, yet bigheaded carp densities in this river are among the highest recorded on the continent. Understanding the causative factors behind erratic recruitment in this commercially-harvested invasive species is important for both limiting their spread and managing their harvest. We analyzed weekly catch records from 15 years of a standardized monitoring program to document the emergence of age-0 bigheaded carp in relation to environmental conditions. The appearance of age-0 fish was generally linked to hydrographic attributes, which probably serve as a cue for spawning. However, we found profound differences in the number of age-0 fish among years, which varied by as much as five orders of magnitude in successive years. The strong link between summer flooding and age-0 fish production we observed emphasizes the importance of understanding the hydrologic context in which sustained invasions occur. Despite evidence of sporadic recruitment, bigheaded carp populations in the Illinois River appear to be consistent or increasing because of particularly strong, episodic year classes. PMID:28929010

Genetic diversity and population structure inferred from the partially duplicated genome of domesticated carp, Cyprinus carpio L.

PubMed

David, Lior; Rosenberg, Noah A; Lavi, Uri; Feldman, Marcus W; Hillel, Jossi

2007-01-01

Genetic relationships among eight populations of domesticated carp (Cyprinus carpio L.), a species with a partially duplicated genome, were studied using 12 microsatellites and 505 AFLP bands. The populations included three aquacultured carp strains and five ornamental carp (koi) variants. Grass carp (Ctenopharyngodon idella) was used as an outgroup. AFLP-based gene diversity varied from 5% (grass carp) to 32% (koi) and reflected the reasonably well understood histories and breeding practices of the populations. A large fraction of the molecular variance was due to differences between aquacultured and ornamental carps. Further analyses based on microsatellite data, including cluster analysis and neighbor-joining trees, supported the genetic distinctiveness of aquacultured and ornamental carps, despite the recent divergence of the two groups. In contrast to what was observed for AFLP-based diversity, the frequency of heterozygotes based on microsatellites was comparable among all populations. This discrepancy can potentially be explained by duplication of some loci in Cyprinus carpio L., and a model that shows how duplication can increase heterozygosity estimates for microsatellites but not for AFLP loci is discussed. Our analyses in carp can help in understanding the consequences of genotyping duplicated loci and in interpreting discrepancies between dominant and co-dominant markers in species with recent genome duplication.

Genetic diversity and population structure inferred from the partially duplicated genome of domesticated carp, Cyprinus carpio L.

PubMed Central

David, Lior; Rosenberg, Noah A; Lavi, Uri; Feldman, Marcus W; Hillel, Jossi

2007-01-01

Genetic relationships among eight populations of domesticated carp (Cyprinus carpio L.), a species with a partially duplicated genome, were studied using 12 microsatellites and 505 AFLP bands. The populations included three aquacultured carp strains and five ornamental carp (koi) variants. Grass carp (Ctenopharyngodon idella) was used as an outgroup. AFLP-based gene diversity varied from 5% (grass carp) to 32% (koi) and reflected the reasonably well understood histories and breeding practices of the populations. A large fraction of the molecular variance was due to differences between aquacultured and ornamental carps. Further analyses based on microsatellite data, including cluster analysis and neighbor-joining trees, supported the genetic distinctiveness of aquacultured and ornamental carps, despite the recent divergence of the two groups. In contrast to what was observed for AFLP-based diversity, the frequency of heterozygotes based on microsatellites was comparable among all populations. This discrepancy can potentially be explained by duplication of some loci in Cyprinus carpio L., and a model that shows how duplication can increase heterozygosity estimates for microsatellites but not for AFLP loci is discussed. Our analyses in carp can help in understanding the consequences of genotyping duplicated loci and in interpreting discrepancies between dominant and co-dominant markers in species with recent genome duplication. PMID:17433244

Effects of hook plate on shoulder function after treatment of acromioclavicular joint dislocation.

PubMed

Chen, Chang-Hong; Dong, Qi-Rong; Zhou, Rong-Kui; Zhen, Hua-Qing; Jiao, Ya-Jun

2014-01-01

Internal fixation with hook plate has been used to treat acromioclavicular joint dislocation. This study aims to evaluate the effect of its use on shoulder function, to further analyze the contributing factors, and provide a basis for selection and design of improved internal fixation treatment of the acromioclavicular joint dislocation in the future. A retrospective analysis was performed on patients treated with a hook plate for acromioclavicular joint dislocation in our hospital from January 2010 to February 2013. There were 33 cases in total, including 25 males and 8 females, with mean age of 48.27 ± 8.7 years. There were 29 cases of Rockwood type III acromioclavicular dislocation, 4 cases of type V. The Constant-Murley shoulder function scoring system was used to evaluate the shoulder function recovery status after surgery. Anteroposterior shoulder X-ray was used to assess the position of the hook plate, status of acromioclavicular joint reduction and the occurrence of postoperative complications. According to the Constant-Murley shoulder function scoring system, the average scores were 78 ± 6 points 8 to 12 months after the surgery and before the removal of the hook plate, the average scores were 89 ± 5 minutes two months after the removal of hook plate. Postoperative X-ray imaging showed osteolysis in 10 cases (30.3%), osteoarthritis in six cases (18.1%), osteolysis associated with osteoarthritis in four cases(12.1%), and steel hook broken in one case (3%). The use of hook plate on open reduction and internal fixation of the acromioclavicular joint dislocation had little adverse effect on shoulder function and is an effective method for the treatment of acromioclavicular joint dislocation. Osteoarthritis and osteolysis are the two common complications after hook plate use, which are associated with the impairment of shoulder function. Shoulder function will be improved after removal of the hook plate.

Covariant Formulation of Hooke's Law.

ERIC Educational Resources Information Center

Gron, O.

1981-01-01

Introducing a four-vector strain and a four-force stress, Hooke's law is written as a four-vector equation. This formulation is shown to clarify seemingly paradoxical results in connection with uniformly accelerated motion, and rotational motion with angular acceleration. (Author/JN)

High-dose hook effect in six automated human chorionic gonadotrophin assays.

PubMed

Al-Mahdili, Huda A; Jones, Graham R D

2010-07-01

The high-dose hook effect is a well-known phenomenon of two-site immunoassays including those for human chorionic gonadotrophin (hCG). We investigated the occurrence of a high-dose hook effect in six routinely available hCG assays using a sample with a total hCG concentration of approximately 3,600,000 IU/L. Dilutions of a sample with high hCG concentration were analysed using six common methods: Advia Centaur, Immulite 2000, Dimension RxL, Unicel DxI 800, Roche E170 and Abbott Architect. The measured concentrations and corresponding assay signals were obtained for each method. Performance was compared with manufacturer claims. Four of the tested platforms demonstrated a clear high-dose hook effect, while the other methods showed no hook effect at the highest level tested. Our results indicate that the hook effect may occur in some hCG assays, although the risk of reporting falsely low results was in most cases at higher concentrations than those indicated in manufacturers' product information. Assay design plays a major role in its occurrence. Laboratories should be aware of the assay limitations in this regard.

Water guns affect abundance and behavior of bigheaded carp and native fish differently

USGS Publications Warehouse

Rivera, Jose; Glover, David C.; Kocovsky, Patrick; Garvey, James E.; Gaikowski, Mark; Jensen, Nathan R.; Adams, Ryan F.

2018-01-01

Water guns have shown the potential to repel nuisance aquatic organisms. This study examines the effects of exposure to a 1966.4 cm3 seismic water gun array (two guns) on the abundance and behavior of Bighead Carp Hypophthalmichthys nobilis, Silver Carp H. molitrix (collectively referred to as bigheaded carp) and native fishes (e.g., Smallmouth Buffalo Ictiobus bubalus). Water guns were deployed in a channel that connects the Illinois River to backwater quarry pits that contained a large transient population of bigheaded carp. To evaluate the effect of water guns, mobile side-looking split-beam hydroacoustic surveys were conducted before, during and between replicated water gun firing periods. Water guns did not affect abundance of bigheaded carp, but abundance of native fish detected during the firing treatment was 43 and 34% lower than the control and water guns off treatments, respectively. The proximity of bigheaded carp to the water gun array was similar between the water guns on and water guns off treatments. In contrast, the closest detected native fish were detected farther from the water guns during the water guns on treatment (mean ± SE, 32.38 ± 3.32 m) than during the water guns off treatment (15.04 ± 1.59 m). The water gun array had a greater impact on native fish species than on bigheaded carp. Caution should be taken to the extrapolation of these results to other fish species and to fish exposed to water guns in different environments (e.g., reduced shoreline interaction) or exposure to a larger array of water guns, or for use of water guns for purposes other than a barrier.

Effects of water hardness on size and hatching success of silver carp eggs

USGS Publications Warehouse

Rach, Jeff J.; Sass, Greg G.; Luoma, James A.; Gaikowski, Mark P.

2010-01-01

Eggs of silver carp Hypophthalmichthys molitrix absorb water after release from the female, causing them to become turgid and to increase substantially in size. The volume of water that diffuses within an egg is most likely determined by (1) the difference in ionic concentration between the egg and the water that surrounds it and (2) the elasticity of the egg membrane. Prior observations suggest that silver carp eggs may swell and burst in soft waters. If water hardness affects silver carp reproductive success in nonnative ecosystems, this abiotic factor could limit silver carp distribution or abundance. In this study, we tested the effect of water hardness on silver carp egg enlargement and hatching success. Groups of newly fertilized silver carp eggs were placed in water at one of five nominal water hardness levels (50, 100, 150, 200, or 250 mg/L as CaCO3) for 1 h to harden (absorb water after fertilization). Egg groups were then placed in separate incubation vessels housed in two recirculation systems that were supplied with either soft (50 mg/L as CaCO3) or hard (250 mg/L as CaCO3) water to evaluate hatching success. Tests were terminated within 24 h after viable eggs had hatched. Eggs that were initially placed in 50-mg/L water to harden were larger (i.e., swelled more) and had a greater probability of hatch than eggs hardened in other water hardness levels. Unlike the effect of water hardness during egg hardening, the water hardness during incubation appeared to have no effect on egg hatching success. Our research suggests that water hardness may not be a limiting factor in the reproduction, recruitment, and range expansion of silver carp in North America.

Morphological analysis of acromion and hook plate for the fixation of acromioclavicular joint dislocation.

PubMed

Yoon, Jong Pil; Lee, Yeon Soo; Song, Geun Soo; Oh, Joo Han

2017-03-01

Acromioclavicular (AC) joint dislocation is a common sports injury. Hook plate fixation is currently widely used to treat this injury, as it can promote the natural healing of the ligament with good clinical outcomes. However, subacromial erosion and impingement are frequently observed post-operatively. It was hypothesized that the morphology and the contact characteristics between the hook portion and the acromion are the main causes of complications after hook plate fixation with the currently available commercial designs. Three-dimensional reconstructed models of the AC joint obtained from the computed tomographic scans of 23 male and 23 female patients (mean age, 61.1 ± 6.3 years) were evaluated, and multiple anatomical parameters were measured. For the subacromial positioning of the hook plate, an actual hook plate (Synthes Inc., West Chester, PA, USA) was scanned, and the contact between the hook plate and the acromion was estimated. The thicknesses of the acromion and distal clavicle were 9.7 ± 1.5 mm (10.7 mm in men; 8.6 mm in women) and 11.3 ± 1.6 mm (11.6 mm in men; 10.0 mm in women), respectively. The width of the acromion was 28.5 ± 3.6 mm. The mean inclination angle between the hook plate and the acromion was 29.3° ± 9.7° (27.9° in men; 30.6° in women). The hook plate made a point contact with the acromion at 9.2 ± 3.3 mm (31.5 %) from the lateral end of the acromion. The results revealed that the hook made a pinpoint contact with the undersurface of the acromion, and this might explain why complications commonly occur after hook plate fixation. The force concentration phenomenon associated with the hook plate of existing designs results from cases of morphological mismatch, such as excessive inclination and improper occupation of the subacromial space.

An experimental 'Life' for an experimental life: Richard Waller's biography of Robert Hooke (1705).

PubMed

Moxham, Noah

2016-03-01

Richard Waller's 'Life of Dr Robert Hooke', prefixed to his edition of Hooke's Posthumous Works (1705), is an important source for the life of one of the most eminent members of the early Royal Society. It also has the distinction of being one of the earliest biographies of a man of science to be published in English. I argue that it is in fact the first biography to embrace the subject's natural-philosophical work as the centre of his life, and I investigate Waller's reasons for adopting this strategy and his struggle with the problem of how to represent an early experimental philosopher in print. I suggest that Waller eschews the 'Christian philosopher' tradition of contemporary biography - partly because of the unusually diverse and fragmentary nature of Hooke's intellectual output - and draws instead upon the structure of the Royal Society's archive as a means of organizing and understanding Hooke's life. The most quoted phrase from Waller's biography is that Hooke became 'to a crime close and reserved' in later life; this essay argues that Waller's biographical sketch was fashioned in order to undo the effects of that reserve. In modelling his approach very closely on the structure of the society's records he was principally concerned with making Hooke's work and biography accessible, intelligible and useful to the fellowship in a context familiar to them, a context which had provided the institutional framework for most of Hooke's adult life. I argue that Waller's 'Life' was also intended to make the largest claims for Hooke's intellectual standing that the author dared in the context of the enmity between Hooke and Isaac Newton once the latter became president of the Royal Society. However, I also adduce fresh manuscript evidence that Waller actually compiled, but did not publish, a defence of Hooke's claim to have discovered the inverse square law of gravity, allowing us to glimpse a much more assertive biography of Hooke than the published version.

Immune-relevant thrombocytes of common carp undergo parasite-induced nitric oxide-mediated apoptosis.

PubMed

Fink, Inge R; Ribeiro, Carla M S; Forlenza, Maria; Taverne-Thiele, Anja; Rombout, Jan H W M; Savelkoul, Huub F J; Wiegertjes, Geert F

2015-06-01

Common carp thrombocytes account for 30-40% of peripheral blood leukocytes and are abundant in the healthy animals' spleen, the thrombopoietic organ. We show that, ex vivo, thrombocytes from healthy carp express a large number of immune-relevant genes, among which several cytokines and Toll-like receptors, clearly pointing at immune functions of carp thrombocytes. Few studies have described the role of fish thrombocytes during infection. Carp are natural host to two different but related protozoan parasites, Trypanoplasma borreli and Trypanosoma carassii, which reside in the blood and tissue fluids. We used the two parasites to undertake controlled studies on the role of fish thrombocytes during these infections. In vivo, but only during infection with T. borreli, thrombocytes were massively depleted from the blood and spleen leading to severe thrombocytopenia. Ex vivo, addition of nitric oxide induced a clear and rapid apoptosis of thrombocytes from healthy carp, supporting a role for nitric oxide-mediated control of immune-relevant thrombocytes during infection with T. borreli. The potential advantage for parasites to selectively deplete the host of thrombocytes via nitric oxide-induced apoptosis is discussed. Copyright © 2015 Elsevier Ltd. All rights reserved.

The LIM Protein Zyxin Binds CARP-1 and Promotes Apoptosis

PubMed Central

Hervy, Martial; Hoffman, Laura M.; Jensen, Christopher C.; Smith, Mark; Beckerle, Mary C.

2010-01-01

Zyxin is a dual-function LIM domain protein that regulates actin dynamics in response to mechanical stress and shuttles between focal adhesions and the cell nucleus. Here we show that zyxin contributes to UV-induced apoptosis. Exposure of wild-type fibroblasts to UV-C irradiation results in apoptotic cell death, whereas cells harboring a homozygous disruption of the zyxin gene display a statistically significant survival advantage. To gain insight into the molecular mechanism by which zyxin promotes apoptotic signaling, we expressed an affinity-tagged zyxin variant in zyxin-null cells and isolated zyxin-associated proteins from cell lysates under physiological conditions. A 130-kDa protein that was co-isolated with zyxin was identified by microsequence analysis as the Cell Cycle and Apoptosis Regulator Protein-1 (CARP-1). CARP-1 associates with the LIM region of zyxin. Zyxin lacking the CARP-1 binding region shows reduced proapoptotic activity in response to UV-C irradiation. We demonstrate that CARP-1 is a nuclear protein. Zyxin is modified by phosphorylation in cells exposed to UV-C irradiation, and nuclear accumulation of zyxin is induced by UV-C exposure. These findings highlight a novel mechanism for modulating the apoptotic response to UV irradiation. PMID:20852740

Clavicular hook plate for grade-III acromioclavicular dislocation.

PubMed

Steinbacher, Gilbert; Sallent, Andrea; Seijas, Roberto; Boffa, Juan Manuel; Espinosa, Wenceslao; Cugat, Ramón

2014-12-01

To review the outcomes of clavicular hook plate fixation for grade-III acromioclavicular (AC) dislocation in young athletes. Medical records of 14 male and 5 female athletes aged 18 to 49 (mean, 29) years who underwent fixation with a 3.5-mm-thick, 15-mm-long clavicular hook plate for acute grade-III AC dislocation were reviewed. The visual analogue score for pain and the Constant shoulder score were assessed. The mean follow-up was 4.2 years. The mean visual analogue score for pain was 1.8 (range, 1-3). The Constant shoulder score was excellent (≥ 90) in 14 patients and good (83-89) in 5 patients. All patients achieved range of motion equal to that of the contralateral side at week 5. Sports activities were resumed by month 6 at the latest. The clavicular hook plate for grade- III AC dislocation enables quick return to sports activities and achieves good-to-excellent functional outcome.

When Teachers Aren't Nice: bell hooks and Feminist Pedagogy.

ERIC Educational Resources Information Center

Buffington, Nancy

The recent "feminization" of composition theory and pedagogy has replaced the classroom figure of the authoritative father with an image of a nurturing mother. But as bell hooks and others insist, the classroom is inherently a place of struggle and conflict and the "real world" is even more so. Hooks offers concrete…

Genome wide identification, phylogeny, and expression of bone morphogenetic protein genes in tetraploidized common carp (Cyprinus carpio).

PubMed

Chen, Lin; Dong, Chuanju; Kong, Shengnan; Zhang, Jiangfan; Li, Xuejun; Xu, Peng

2017-09-05

Bone morphogenetic proteins (Bmps) are a group of signaling molecules known to play important roles during formation and maintenance of various organs, not only bone, but also muscle, blood and so on. Common carp (Cyprinus carpio) is one of the most intensively studied fish due to its economic and environmental importance. Besides, common carp has encountered an additional round of whole genome duplication (WGD) compared with many closely related diploid teleost, which make it one of the most important models for genome evolutionary studies in teleost. Comprehensive genome resources of common carp have been developed recently, which facilitate the thorough characterization of bmp gene family in the tetraploidized common carp genome. We identified a total of 44 bmps from the common carp genome, which are twice as many as that of zebrafish. Phylogenetic analysis revealed that most of bmps are highly conserved. Comparative analysis was performed across six typical vertebrate genomes. It appeared that all the bmp genes in common carp were duplicated. Obviously, the expansion of the bmp gene family in common carp was due to the latest additional round of whole genome duplication and made it more abundant than other diploid teleosts. Expression signatures were assessed in major tissues, including gill, intestine, liver, spleen, skin, heart, gonad, muscle, kidney, head kidney, brain and blood, which demonstrated the comprehensive expression profiles of bmp genes in the tetraploidized genome. Significant gene expression divergences were observed which revealed substantial functional divergences of those duplicated bmp genes post the latest WGD event. The conserved synteny blocks of bmp5s revealed the genome rearrangement of common carp post the 4R WGD. The whole set of bmp gene family in common carp provides insight into gene fate of tetraploidized common carp genome post recent WGD. Copyright © 2017. Published by Elsevier B.V.

Using a Population Model to Inform the Management of River Flows and Invasive Carp (Cyprinus carpio).

PubMed

Koehn, John D; Todd, Charles R; Zampatti, Brenton P; Stuart, Ivor G; Conallin, Anthony; Thwaites, Leigh; Ye, Qifeng

2018-03-01

Carp are a highly successful invasive fish species, now widespread, abundant and considered a pest in south-eastern Australia. To date, most management effort has been directed at reducing abundances of adult fish, with little consideration of population growth through reproduction. Environmental water allocations are now an important option for the rehabilitation of aquatic ecosystems, particularly in the Murray-Darling Basin. As carp respond to flows, there is concern that environmental watering may cause floodplain inundation and provide access to spawning habitats subsequently causing unwanted population increase. This is a management conundrum that needs to be carefully considered within the context of contemporary river flow management (natural, environmental, irrigation). This paper uses a population model to investigate flow-related carp population dynamics for three case studies in the Murray-Darling Basin: (1) river and terminal lakes; (2) wetlands and floodplain lakes; and (3) complex river channel and floodplain system. Results highlight distinctive outcomes depending on site characteristics. In particular, the terminal lakes maintain a significant source carp population regardless of river flow; hence any additional within-channel environmental flows are likely to have little impact on carp populations. In contrast, large-scale removal of carp from the lakes may be beneficial, especially in times of extended low river flows. Case studies 2 and 3 show how wetlands, floodplain lakes and the floodplain itself can now often be inundated for several months over the carp spawning season by high volume flows provided for irrigation or water transfers. Such inundations can be a major driver of carp populations, compared to within channel flows that have relatively little effecton recruitment. The use of a population model that incorporates river flows and different habitats for this flow-responsive species, allows for the comparison of likely population

Using a Population Model to Inform the Management of River Flows and Invasive Carp ( Cyprinus carpio)

NASA Astrophysics Data System (ADS)

Koehn, John D.; Todd, Charles R.; Zampatti, Brenton P.; Stuart, Ivor G.; Conallin, Anthony; Thwaites, Leigh; Ye, Qifeng

2018-03-01

Carp are a highly successful invasive fish species, now widespread, abundant and considered a pest in south-eastern Australia. To date, most management effort has been directed at reducing abundances of adult fish, with little consideration of population growth through reproduction. Environmental water allocations are now an important option for the rehabilitation of aquatic ecosystems, particularly in the Murray-Darling Basin. As carp respond to flows, there is concern that environmental watering may cause floodplain inundation and provide access to spawning habitats subsequently causing unwanted population increase. This is a management conundrum that needs to be carefully considered within the context of contemporary river flow management (natural, environmental, irrigation). This paper uses a population model to investigate flow-related carp population dynamics for three case studies in the Murray-Darling Basin: (1) river and terminal lakes; (2) wetlands and floodplain lakes; and (3) complex river channel and floodplain system. Results highlight distinctive outcomes depending on site characteristics. In particular, the terminal lakes maintain a significant source carp population regardless of river flow; hence any additional within-channel environmental flows are likely to have little impact on carp populations. In contrast, large-scale removal of carp from the lakes may be beneficial, especially in times of extended low river flows. Case studies 2 and 3 show how wetlands, floodplain lakes and the floodplain itself can now often be inundated for several months over the carp spawning season by high volume flows provided for irrigation or water transfers. Such inundations can be a major driver of carp populations, compared to within channel flows that have relatively little effecton recruitment. The use of a population model that incorporates river flows and different habitats for this flow-responsive species, allows for the comparison of likely population

Evaluation of the orientation of 90* and 180* reinforcing bar hooks.

DOT National Transportation Integrated Search

2012-01-01

This report describes test results of a study initiated to evaluate the potential influence of hook tilt angle of standard reinforcing hooks : on the bond strength of concrete. The topic of the evaluation of the orientation of 90 and 180 degree reinf...

Acoustic deterrence of bighead carp (Hypophthalmichthys nobilis) to a broadband sound stimulus

USGS Publications Warehouse

Vetter, Brooke J.; Murchy, Kelsie; Cupp, Aaron R.; Amberg, Jon J.; Gaikowski, Mark P.; Mensinger, Allen F.

2017-01-01

Recent studies have shown the potential of acoustic deterrents against invasive silver carp (Hypophthalmichthys molitrix). This study examined the phonotaxic response of the bighead carp (H. nobilis) to pure tones (500–2000 Hz) and playbacks of broadband sound from an underwater recording of a 100 hp outboard motor (0.06–10 kHz) in an outdoor concrete pond (10 × 5 × 1.2 m) at the U.S. Geological Survey Upper Midwest Environmental Science Center in La Crosse, WI. The number of consecutive times the fish reacted to sound from alternating locations at each end of the pond was assessed. Bighead carp were relatively indifferent to the pure tones with median consecutive responses ranging from 0 to 2 reactions away from the sound source. However, fish consistently exhibited significantly (P < 0.001) greater negative phonotaxis to the broadband sound (outboard motor recording) with an overall median response of 20 consecutive reactions during the 10 min trials. In over 50% of broadband sound tests, carp were still reacting to the stimulus at the end of the trial, implying that fish were not habituating to the sound. This study suggests that broadband sound may be an effective deterrent to bighead carp and provides a basis for conducting studies with wild fish.

Isotope niche dimension and trophic overlap between bigheaded carps and native filter-feeding fish in the lower Missouri River, USA

USGS Publications Warehouse

Wang, Jianzhu; Chapman, Duane C.; Xu, Jun; Wang, Yang; Gu, Binhe

2018-01-01

Stable carbon and nitrogen isotope values (δ13C and δ15N) were used to evaluate trophic niche overlap between two filter-feeding fishes (known together as bigheaded carp) native to China, silver carp (Hypophthalmichthys molitrix) and bighead carp (Hypophthalmichthys nobilis), and three native filter-feeding fish including bigmouth buffalo (Ictiobus cyprinellus), gizzard shad (Dorosoma cepedianum) and paddlefish (Polyodon spathula) in the lower Missouri River, USA, using the Bayesian Stable Isotope in R statistics. Results indicate that except for bigmouth buffalo, all species displayed similar trophic niche size and trophic diversity. Bigmouth buffalo occupied a small trophic niche and had the greatest trophic overlap with silver carp (93.6%) and bighead carp (94.1%) followed by gizzard shad (91.0%). Paddlefish had a trophic niche which relied on some resources different from those used by other species, and therefore had the lowest trophic overlap with bigheaded carp and other two native fish. The trophic overlap by bigheaded carp onto native fish was typically stronger than the reverse effects from native fish. Average niche overlap between silver carp and native species was as high as 71%, greater than niche overlap between bighead carp and native fish (64%). Our findings indicate that bigheaded carps are a potential threat to a diverse and stable native fish community.

Effects of antimicrobial peptides (AMPs) on blood biochemical parameters, antioxidase activity, and immune function in the common carp (Cyprinus carpio).

PubMed

Dong, Xiao-Qing; Zhang, Dong-Ming; Chen, Yu-Ke; Wang, Qiu-Ju; Yang, Yi-Yu

2015-11-01

Antibiotic use in livestock feed additives has resulted in harmful residue accumulation and spread of drug-resistance. We examined the use of antimicrobial peptides (AMPs) as a safer alternative to antibiotics in feeding the common carp. AMPs were added to common carp basal diets (Control) as additives at four concentrations: 100 mg kg(-1) (B1), 200 mg kg(-1) (B2), 400 mg kg(-1) (B3), 600 mg kg(-1) (B4) by dry weight of basal diet. After a 60-day feeding experiment, the final weight, DG and SGR of carps on B1, B2 and B3 diet were significantly higher than the control (p < 0.05). The FCR of carps on B1, B2 and B3 diet were significantly lower than the control (p < 0.05). Carps on B2, B3, and B4 diets showed significantly lower (p < 0.05) levels of triglyceride than the control. B4-fed carps showed significantly lower (p < 0.05) levels of total protein, albumin, and total cholesterol than the control. However there was no remarkable difference (p > 0.05) in levels of uric ammonia, globulin, glutamic-pyruvic transaminase, glutamic-oxalacetic transaminase, lactic dehydrogenase and blood glucose in all groups. The serum superoxide dismutase and catalase activity of B1-fed carps was significantly higher (p < 0.05) than the control and B4-fed carps. The serum alkaline phosphate activity of carps on B1 diets was significantly higher (p < 0.05) than B4-fed carps. The serum acid phosphatase activity of B1-fed carps was significantly higher (p < 0.05) than the control and other antimicrobial peptide-fed groups. The serum lysozyme activity of carps on B1, B2, and B3 diets was significantly higher (p < 0.05) than the control- and B4-fed carps. Regarding immune factors in serum, the levels of immunoglobulin (Ig) and interleukin (IL)-1β in B1-fed carps were significantly higher (p < 0.05) than the control and other groups, while IL-1α levels in B1-fed carps was significantly higher (p < 0.05) than the control-, B2-, and B3-fed carps. Furthermore, there were no significant

Flagellar Hook Flexibility Is Essential for Bundle Formation in Swimming Escherichia coli Cells

PubMed Central

Brown, Mostyn T.; Steel, Bradley C.; Silvestrin, Claudio; Wilkinson, David A.; Delalez, Nicolas J.; Lumb, Craig N.; Obara, Boguslaw; Berry, Richard M.

2012-01-01

Swimming Escherichia coli cells are propelled by the rotary motion of their flagellar filaments. In the normal swimming pattern, filaments positioned randomly over the cell form a bundle at the posterior pole. It has long been assumed that the hook functions as a universal joint, transmitting rotation on the motor axis through up to ∼90° to the filament in the bundle. Structural models of the hook have revealed how its flexibility is expected to arise from dynamic changes in the distance between monomers in the helical lattice. In particular, each of the 11 protofilaments that comprise the hook is predicted to cycle between short and long forms, corresponding to the inside and outside of the curved hook, once each revolution of the motor when the hook is acting as a universal joint. To test this, we genetically modified the hook so that it could be stiffened by binding streptavidin to biotinylated monomers, impeding their motion relative to each other. We found that impeding the action of the universal joint resulted in atypical swimming behavior as a consequence of disrupted bundle formation, in agreement with the universal joint model. PMID:22522898

Asian Carp Survivability Experiments and Water Transport Surveys in the Illinois River, Volume 1

DTIC Science & Technology

2013-01-01

developmental stages of the eggs and larvae of the four species of Asian carp in the Yangtze River. These stages begin with egg fertilization and end with the...hatching in the Yangtze River after about 38 hours. Development to the juvenile stage in silver and bighead carp was estimated to take about 60-70 days...Native to the Yangtze River, China. U.S. Geological Survey Data Series 239, 51 pp. Coulter, A. & Goforth, R. R. 2011. Silver and Bighead Carp

Assessment of phytoplankton resources suitable for bigheaded carps in Lake Michigan derived from remote sensing and bioenergetics

USGS Publications Warehouse

Anderson, Karl R.; Chapman, Duane C.; Wynne, Tim T.; Paukert, Craig P.

2017-01-01

We used bioenergetic simulations combined with satellite-measured water temperature and estimates of algal food availability to predict the habitat suitability of Lake Michigan for adult silver carp (Hypophthalmichthys molitrix) and bighead carp (H. nobilis). Depending on water temperature, we found that bigheaded carp require ambient algal concentrations between 1 and 7 μg chlorophyll/L or between 0.25 × 105 and 1.20 × 105 cells/mL Microcystis to maintain body weight. When the bioenergetics model is forced with the observed average annual temperature cycle, our simulations predicted silver carp bioenergetics predicted annual weight change ranging from 9% weight loss to 23% gain; bighead carp ranged from 68 to 177% weight gain. Algal concentrations b4 μg chlorophyll/L and b200,000 cells/mL were below the detection limits of the remote sensing method. However, all areas with detectable algae have sufficient concentrations of algal foods for bigheaded carp weight-maintenance and growth. Those areas are predominately along the nearshore areas.

77 FR 15722 - Southern California Hook and Line Survey; Public Meeting

Federal Register 2010, 2011, 2012, 2013, 2014

2012-03-16

... meeting to evaluate the Southern California Shelf Rockfish Hook and Line Survey which was designed to... and Line survey design and protocols; (2) examine the analytical methods used to generate rockfish... California Hook and Line Survey; Public Meeting AGENCY: National Marine Fisheries Service (NMFS), National...

Disseminated Mycobacterium intracellulare infection in a broad-snouted caiman Caiman latirostris.

PubMed

Kik, Marja J L

2013-11-25

A 10 yr old broad-snouted caiman Caiman latirostris from a small Dutch animal park was presented with long-term variable periods of anorexia and weight loss. Blood chemistry showed slightly elevated uric acid levels and low ionised calcium concentration. Ultrasonographical thickening of the intestinal wall in the region of the duodenum was evident. Pathological changes were a thickening of the wall of 90% of the small intestines, enlarged spleen with multifocal white foci and an enlarged light-brown liver. Histopathological lesions consisted of disseminated granulomas in the intestinal wall, the liver and the spleen. Multinucleated giant cells and epitheloid macrophages were abundant. Ziehl-Neelsen staining showed numerous intralesional acid-fast bacteria. Polymerase chain reaction for Mycobacterium intracellulare was positive.

Effects of hook plate on shoulder function after treatment of acromioclavicular joint dislocation

PubMed Central

Chen, Chang-Hong; Dong, Qi-Rong; Zhou, Rong-Kui; Zhen, Hua-Qing; Jiao, Ya-Jun

2014-01-01

Introduction: Internal fixation with hook plate has been used to treat acromioclavicular joint dislocation. This study aims to evaluate the effect of its use on shoulder function, to further analyze the contributing factors, and provide a basis for selection and design of improved internal fixation treatment of the acromioclavicular joint dislocation in the future. Methods: A retrospective analysis was performed on patients treated with a hook plate for acromioclavicular joint dislocation in our hospital from January 2010 to February 2013. There were 33 cases in total, including 25 males and 8 females, with mean age of 48.27 ± 8.7 years. There were 29 cases of Rockwood type III acromioclavicular dislocation, 4 cases of type V. The Constant-Murley shoulder function scoring system was used to evaluate the shoulder function recovery status after surgery. Anteroposterior shoulder X-ray was used to assess the position of the hook plate, status of acromioclavicular joint reduction and the occurrence of postoperative complications. Results: According to the Constant-Murley shoulder function scoring system, the average scores were 78 ± 6 points 8 to 12 months after the surgery and before the removal of the hook plate, the average scores were 89 ± 5 minutes two months after the removal of hook plate. Postoperative X-ray imaging showed osteolysis in 10 cases (30.3%), osteoarthritis in six cases (18.1%), osteolysis associated with osteoarthritis in four cases(12.1%), and steel hook broken in one case (3%). Conclusion: The use of hook plate on open reduction and internal fixation of the acromioclavicular joint dislocation had little adverse effect on shoulder function and is an effective method for the treatment of acromioclavicular joint dislocation. Osteoarthritis and osteolysis are the two common complications after hook plate use, which are associated with the impairment of shoulder function. Shoulder function will be improved after removal of the hook plate. PMID

Preservation of the genetic diversity of a local common carp in the agricultural heritage rice–fish system

PubMed Central

Ren, Weizheng; Hu, Liangliang; Guo, Liang; Zhang, Jian; Tang, Lu; Zhang, Entao; Zhang, Jiaen; Luo, Shiming; Tang, Jianjun; Chen, Xin

2018-01-01

We examined how traditional farmers preserve the genetic diversity of a local common carp (Cyprinus carpio), which is locally referred to as “paddy field carp” (PF-carp), in a “globally important agricultural heritage system” (GIAHS), i.e., the 1,200-y-old rice–fish coculture system in Zhejiang Province, China. Our molecular and morphological analysis showed that the PF-carp has changed into a distinct local population with higher genetic diversity and diverse color types. Within this GIAHS region, PF-carps exist as a continuous metapopulation, although three genetic groups could be identified by microsatellite markers. Thousands of small farmer households interdependently obtained fry and parental carps for their own rice–fish production, resulting in a high gene flow and large numbers of parent carps distributing in a mosaic pattern in the region. Landscape genetic analysis indicated that farmers’ connectivity was one of the major factors that shaped this genetic pattern. Population viability analysis further revealed that the numbers of these interconnected small farmer households and their connection intensity affect the carps’ inherent genetic diversity. The practice of mixed culturing of carps with diverse color types helped to preserve a wide range of genetic resources in the paddy field. This widespread traditional practice increases fish yield and resource use, which, in return, encourages famers to continue their practice of selecting and conserving diverse color types of PF-carp. Our results suggested that traditional farmers secure the genetic diversity of PF-carp and its viability over generations in this region through interdependently incubating and mixed-culturing practices within the rice−fish system. PMID:29295926

Location and timing of Asian carp spawning in the Lower Missouri River

USGS Publications Warehouse

Deters, Joseph E.; Chapman, Duane C.; McElroy, Brandon

2013-01-01

We sampled for eggs of Asian carps, (bighead carp Hypophthalmichthys nobilis, silver carp H. molitrix, and grass carp Ctenopharyngodon idella) in 12 sites on the Lower Missouri River and in six tributaries from the months of May through July 2005 and May through June 2006 to examine the spatial and temporal dynamics of spawning activity. We categorized eggs into thirty developmental stages, but usually they could not be identified to species. We estimated spawning times and locations based on developmental stage, temperature dependent rate of development and water velocity. Spawning rate was higher in the daytime between 05:00 and 21:00 h than at night. Spawning was not limited to a few sites, as has been reported for the Yangtze River, where these fishes are native, but more eggs were spawned in areas of high sinuosity. We employ a sediment transport model to estimate vertical egg concentration profiles and total egg fluxes during spawning periods on the Missouri River. We did not identify substantial spawning activity within tributaries or at tributary confluences examined in this study.

Removal of a barbed fish hook from the cornea of an 8-year-old boy.

PubMed

Kemp, Pavlina S; Shah, Ankoor S; Elliott, Alexandra T; Wan, Michael J

2017-10-01

This video article shows the surgical removal of a three-pronged barbed fish hook from the cornea and anterior chamber of an 8-year-old boy. The advance-and-cut method is shown: the embedded barbed point of the fish hook was advanced through the cornea, its point was cut off, and the shank of the hook was withdrawn. This method was chosen due to the three-pronged configuration of the fish hook to minimize additional trauma to the eye. Other methods of fish hook removal are briefly reviewed. Copyright © 2017 American Association for Pediatric Ophthalmology and Strabismus. Published by Elsevier Inc. All rights reserved.

Are College Students Replacing Dating and Romantic Relationships with Hooking Up?

ERIC Educational Resources Information Center

Siebenbruner, Jessica

2013-01-01

This study assessed female college students' ("N" = 197) participation in dating, romantic relationships, hooking up behaviors, and the intersection of these activities. Hooking up was prevalent among students ("n" = 78; 39.6%), but dating ("n" = 139; 70.6%) and romantic relationship ("n" = 147; 74.6%)…

Hormonal regulation of hepatic glycogenolysis in the carp, Cyprinus carpio

DOE Office of Scientific and Technical Information (OSTI.GOV)

Janssens, P.A.; Lowrey, P.

1987-04-01

Carp (Cyprinus carpio) liver maintained normal glycogen content and enzyme complement for several days in organ culture. Epinephrine-stimulated glycogenolysis, phosphorylase activation, and cyclic AMP (cAMP) accumulation in a concentration-dependent manner with EC/sub 50/s of 100, 100, and 500 nM, respectively. These actions were blocked by the ..beta..-adrenergic antagonist, propranolol, but not by the ..cap alpha..-adrenergic antagonist phentolamine. Glycogenolysis and tissue cAMP were uninfluenced by 10/sup -6/ M arginine vasotocin, arginine vasopressin, lysine vasotocin, lysine vasopressin, mesotocin, or oxytocin, but were slightly increased by 10/sup -5/ M isotocin and slightly decreased by 10/sup -6/ M angiotensin II. (/sup 125/I)-iodocyanopindolol (ICP), amore » ..beta..-adrenergic ligand, bound to isolated carp liver membranes with a K/sub D/ of 83 pM. Maximum binding of 45 fmol/mg protein was at 600 pM. Propranolol, isoprenaline, epinephrine, phenylephrine, norepinephrine, and phenoxybenzamine displaced ICP with K/sub D/s of 100 nM, 2, 20, 20, 60, and 200 ..mu..M, respectively. The ..cap alpha..-adrenergic antagonists, yohimbine and prazosin, showed no specific binding. These data provide evidence that catecholamines act via ..beta..-adrenergic receptors in carp liver and that ..cap alpha..-adrenergic receptors are not present. Vasoactive peptides play no significant role in regulation of carp liver glycogenolysis.« less

Fluidically Controlled Cargo Hook

DTIC Science & Technology

1975-03-01

Final Breadboard Fluidic Circuit IT 6 External Cargo Handling System - Cü-Sk Type Aircraft 18 7 Back Pressure Switch Response Time - Switching...On 20 8 Back Pressure Switch Response Time - Switching Off 21 9 Hook Actuator - Pressure Rise Rate 22 10 Breadboard Fluidic System Component...LINE LENGTH* FT Figure 7« Back. Pressure Switch Response Time - Switching On. ! TABLE k. INTERFACE VALVE SIGNAL TIME

The impact of using chickpea flour and dried carp fish powder on pizza quality.

PubMed

El-Beltagi, Hossam S; El-Senousi, Naglaa A; Ali, Zeinab A; Omran, Azza A

2017-01-01

Pizza being the most popular food worldwide, quality and sensory appeal are important considerations during its modification effort. This study was aimed to evaluate the quality of pizza made using two different sources of proteins, chickpea (Cicer arietinum) flour and dried carp fish powder (Cyprinus carpio). Analysis indicated nutrients richness specificity of chickpea flour (higher fiber, energy, iron, zinc, linoleic acid and total nonessential amino acids) and dried carp fish powder (higher contents of protein, fats, ash, oleic acid and total essential amino acids) complementing wheat flour to enhance nutritional value of pizza. Total plate count and thiobarbituric acid were increased (P<0.05) in dried carp fish powder after 45 days of storage, but no Coliform were detected. Wheat flour was substituted with 5, 7.5 and 10% chickpea flour or dried carp fish powder and chemical, textural, sensory and storage evaluation parameters of in pizza were investigated. Dried carp fish powder increased (P<0.05) contents of protein, ash, fats, zinc and protein digestibility of pizza. Chickpea flour increased iron and zinc contents of the pizza. Water activity (aw) was decreased in fish powder and chickpea pizza. Pizza firmness and gumminess were significantly (p<0.05) increased at every level of protein source, but cohesiveness was decreased with 10% chickpea flour. Pizza chewiness was the same (P>0.05) across the levels of two protein sources. Springiness was decreased (P<0.05) with high level (10%) dried fish powder and low/intermediate level of chickpea flour. Chickpea and dried carp fish incorporation up to 7.50% in pizza at the expense of wheat flour had no effect (P>0.05) on all sensorial parameters except for odor values. The results could be useful in utilization of chickpea flour and carp fish powder in designing nutritious pizza for consumers.

The impact of using chickpea flour and dried carp fish powder on pizza quality

PubMed Central

El-Senousi, Naglaa A.; Ali, Zeinab A.; Omran, Azza A.

2017-01-01

Pizza being the most popular food worldwide, quality and sensory appeal are important considerations during its modification effort. This study was aimed to evaluate the quality of pizza made using two different sources of proteins, chickpea (Cicer arietinum) flour and dried carp fish powder (Cyprinus carpio). Analysis indicated nutrients richness specificity of chickpea flour (higher fiber, energy, iron, zinc, linoleic acid and total nonessential amino acids) and dried carp fish powder (higher contents of protein, fats, ash, oleic acid and total essential amino acids) complementing wheat flour to enhance nutritional value of pizza. Total plate count and thiobarbituric acid were increased (P<0.05) in dried carp fish powder after 45 days of storage, but no Coliform were detected. Wheat flour was substituted with 5, 7.5 and 10% chickpea flour or dried carp fish powder and chemical, textural, sensory and storage evaluation parameters of in pizza were investigated. Dried carp fish powder increased (P<0.05) contents of protein, ash, fats, zinc and protein digestibility of pizza. Chickpea flour increased iron and zinc contents of the pizza. Water activity (aw) was decreased in fish powder and chickpea pizza. Pizza firmness and gumminess were significantly (p<0.05) increased at every level of protein source, but cohesiveness was decreased with 10% chickpea flour. Pizza chewiness was the same (P>0.05) across the levels of two protein sources. Springiness was decreased (P<0.05) with high level (10%) dried fish powder and low/intermediate level of chickpea flour. Chickpea and dried carp fish incorporation up to 7.50% in pizza at the expense of wheat flour had no effect (P>0.05) on all sensorial parameters except for odor values. The results could be useful in utilization of chickpea flour and carp fish powder in designing nutritious pizza for consumers. PMID:28873098

Analysis of the skin transcriptome in two oujiang color varieties of common carp.

PubMed

Wang, Chenghui; Wachholtz, Michael; Wang, Jun; Liao, Xiaolin; Lu, Guoqing

2014-01-01

Body color and coloration patterns are important phenotypic traits to maintain survival and reproduction activities. The Oujiang color varieties of common carp (Cyprinus carpio var. color), with a narrow distribution in Zhejiang Province of China and a history of aquaculture for over 1,200 years, consistently exhibit a variety of body color patterns. The molecular mechanism underlying diverse color patterns in these variants is unknown. To the practical end, it is essential to develop molecular markers that can distinguish different phenotypes and assist selective breeding. In this exploratory study, we conducted Roche 454 transcriptome sequencing of two pooled skin tissue samples of Oujiang common carp, which correspond to distinct color patterns, red with big black spots (RB) and whole white (WW), and a total of 737,525 sequence reads were generated. The reads obtained in this study were co-assembled jointly with common carp Roche 454 sequencing reads downloaded from NCBI SRA database, resulting in 43,923 isotigs and 546,676 singletons. Over 31 thousand (31,445; 71.6%) isotigs were found with significant BLAST matches (E<1e-10) to the nr protein database, which corresponds to 12,597 annotated zebrafish genes. A total of 70,947 isotigs and singletons (transcripts) were annotated with Gene Ontology, and 60,221 transcripts were found with corresponding EC numbers. Out of 145 zebrafish pigmentation genes, orthologs for 117 were recovered in Oujiang color carp transcriptome, including 18 found only among singletons. Our transcriptome analysis revealed over 52,902 SNPs in Oujiang common carp, and identified 63 SNP markers that are putatively unique either for RB or WW. The transcriptome of Oujiang color varieties of common carp obtained through this study, along with the pigmentation genes recovered and the color pattern-specific molecular markers developed, will facilitate future research on the molecular mechanism of color patterns and promote aquaculture of Oujiang

Investigation of zoonotic disease pathogens (Aeromonas hydrophila, Pseudomonas fluorescens, Streptococcus iniae) seen in carp farms in the Northern Iraq-Erbil region by molecular methods

NASA Astrophysics Data System (ADS)

Ibraheem, Azad Saber; Önalan, Şükrü; Arabacı, Muhammed

2017-04-01

The aim of this study was to determine the zoonotic bacteria in carp farms in the Northern Iraq-Erbil region. Carp is the main fish species cultured in Erbil region. The most common zoonotic bacteria generally seen in carp farms are Aeromonas hydrophila, Pseudomonas fluorescens and Streptococcus iniae. Samples were collected from 25 carp farms in the Northern Iraq-Erbil region. Six carp samples were collected from each carp farm. Head kidney and intestine tissue samples were collected from each carp sample. Then head kidney and intestine tissue samples were pooled separately from each carp farm. Total bacterial DNA had been extracted from the 25 pooled head kidney and 25 intestinal tissue samples. The pathogen Primers were originally designed from 16S RNA gene region. Zoonotic bacteria were scanned in all tissue samples with absent/present analysis by RT-PCR. Furthermore, the capillary gel electrophoresis bands were used for confirmation of amplicon size which was planned during primer designing stage. As a result, thirteen carp farms were positive in the respect to Aeromonas hydrophila, eight carp farms were positive from head kidney and six carp farms were positive from the intestine, only one carp farm was positive from both head kidney and the intestine tissue samples. In the respect to Streptococcus iniae, four carp farms were positive from head kidney and two carp farms were positive from the intestine. Only one carp farm was positive in the respect to Pseudomonas fluorescens from the intestine. Totally, 9 of 25 carp farms were cleared (negative) the zoonotic bacteria. In conclusion, the zoonotic bacteria were high (64 %) in carp farms in the Northern Iraq-Erbil region.

Light exaggerates apical hook curvature through phytochrome actions in tomato seedlings.

PubMed

Shichijo, Chizuko; Ohuchi, Hisako; Iwata, Naoko; Nagatoshi, Yukari; Takahashi, Miki; Nakatani, Eri; Inoue, Kentaroh; Tsurumi, Seiji; Tanaka, Osamu; Hashimoto, Tohru

2010-02-01

Contrary to the established notion that the apical hook of dark-grown dicotyledonous seedlings opens in response to light, we found in tomato (Solanum lycopersicum L.) that the apical hook curvature is exaggerated by light. Experiments with several tomato cultivars and phytochrome mutants, irradiated with red and far-red light either as a brief pulse (Rp, FRp) or continuously (Rc, FRc), revealed: the hook-exaggeration response is maximal at the emergence of the hypocotyl from the seed; the effect of Rp is FRp-reversible; fluence-response curves to a single Rp or FRp show an involvement of low and very low fluence responses (LFR, VLFR); the effect of Rc is fluence-rate dependent, but that of FRc is not; the phyA mutant (phyA hp-1) failed to respond to an Rp of less than 10(-2) micromol m(-2) and to an FRp of all fluences tested as well as to FRc, thus indicating that the hook-exaggeration response involves phyA-mediated VLFR. The Rp fluence-response curve with the same mutant also confirmed the presence of an LFR mediated by phytochrome(s) other than phyA, although the phyB1 mutant (phyB1 hp-1) still showed full response probably due to other redundant phytochrome species (e.g., phyB2). Simulation experiments led to the possible significance of hook exaggeration in the field that the photoresponse may facilitate the release of seed coat when seeds germinate at some range of depth in soil. It was also observed that seed coat and/or endosperm are essential to the hook exaggeration.

Collaborative Lesson Hook Design in Science Teacher Education: Advancing Professional Practice

ERIC Educational Resources Information Center

McCauley, Veronica; Davison, Kevin; Byrne, Corinna

2015-01-01

This article documents the process of collaboratively developing lesson hook e-resources for science teachers to establish a community of inquiry and to strengthen the pedagogy of science teaching. The authors aim to illustrate how the development and application of strategic hooks can bridge situational interest and personal interest so that…

Assessing the Personal Negative Impacts of Hooking Up Experienced by College Students: Gender Differences and Mental Health.

PubMed

Napper, Lucy E; Montes, Kevin S; Kenney, Shannon R; LaBrie, Joseph W

2016-09-01

Hooking up is a normative behavior among college students that is associated with a range of positive and negative consequences. While previous research has primarily focused on women's negative experiences of hooking up, the current study explored the relationships among hooking up behaviors, psychological distress, and a broad range of negative effects of hooking up in both male and female college students. Using a multisite sample of college students, we developed the 14-item Negative Impact of Hookups Inventory (NIHI) to assess negative health outcomes, emotional responses, and social consequences associated with hooking up. Unprotected sex and having more hookup partners were associated with greater negative experiences of hooking up. Contrary to expectations, there were no gender differences in the total number of negative hookup effects, although men reported more frequent hookups. In addition, negative impacts of hooking up were positively associated with psychological distress regardless of gender. The NIHI may offer a useful tool to assess the negative impacts of hooking up. Understanding students' hookup experiences is an important step toward developing targeted health interventions related to hooking up behavior in young adult populations.

Growth Comparison of Israeli Carp (Cyprinus carpio) to Different Breeding Combination

PubMed Central

Hwang, Ju-ae; Goo, In Bon; Kim, Jung Eun; Kim, Myung Hun; Kim, Do Hee; Im, Jae Hyun; Choi, Hye-Sung; Lee, Jeong-Ho

2016-01-01

ABSTRACT The purpose of this study was to investigate the improvement of growth in Israeli carp (Cyprinus carpio), and the cross experiment was carried out with two strains of Israeli carp. Four combinations of Israeli carp from Jeonbuk fisheries farm and Songpu mirror carp from Heilong Jiang, China (KK; Jeonbuk ♀ × Jeonbuk ♂, KC; Jeonbuk ♀ × China ♂, CC; China ♀ × China ♂ and CK; China ♀ × Jeonbuk ♂) were developed and reared. Body length, body weight and condition factor were determined at 20, 40, 60 and 170 days post-hatch (DPH). The results showed that there were differences in growth rate of the four groups. Body length of four groups were CK > CC > KC > KK and body weight were CC > CK > KC > KK at 170 DPH. The growth perfomance of four groups were statistically significant difference (P<0.05). During the rearing, CC group had longer length and higher weight at 170 DPH compared to other three groups and also condition factor was highest in the CC group, but there was no significant difference in a survival rate. These results indicated that the growth performance mainly depended upon brooder combination but survival rate could not significantly affect brooder. PMID:28144632

Effects of triploid grass carp on aquatic plants, water quality, and public satisfaction in Washington State

USGS Publications Warehouse

Bonar, Scott A.; Bolding, B.; Divens, M.

2002-01-01

We investigated effects of triploid grass carp Ctenopharyngodon idella on aquatic macrophyte communities, water quality, and public satisfaction for 98 lakes and ponds in Washington State stocked with grass carp between 1990 and 1995. Grass carp had few noticeable effects on macrophyte communities until 19 months following stocking. After 19 months, submersed macrophytes were either completely eradicated (39% of the lakes) or not controlled (42% of the lakes) in most lakes. Intermediate control of submersed macrophytes occurred in 18% of lakes at a median stocking rate of 24 fish per vegetated surface acre. Most of the landowners interviewed (83%) were satisfied with the results of introducing grass carp. For sites where all submersed macrophytes were eradicated, average turbidity was higher (11 nephelometric turbidity units, NTU) than at sites where macrophytes were controlled to intermediate levels (4 NTU) or unaffected by grass carp grazing (5 NTU). Chlorophyll a was not significantly different between levels of macrophyte control; therefore, we concluded that most of this turbidity was abiotic and not algal. Triploid grass carp were a popular control option and effectively grazed most submersed macrophytes in Washington State. However, calculating stocking rates based on landowner estimates of aquatic plant coverage rarely resulted in intermediate levels of aquatic plant control. Additionally, the effects of particular stocking rates varied considerably. We recommend against using grass carp in Washington lakes where eradication of submersed vegetation cannot be tolerated.

Assessing the efficacy of corn-based bait containing antimycin-a to control common carp populations using laboratory and pond experiments

USGS Publications Warehouse

Poole, Joshua R.; Sauey, Blake W.; Amberg, Jon J.; Bajer, Przemyslaw G.

2018-01-01

Strategic use of oral toxicants could allow for practical and sustainable control schemes for the invasive common carp (Cyprinus carpio, or ‘carp’) if a toxicant selectively targeted carp and not native species. In this study, we incorporated antimycin-a (ANT-A), a known fish toxicant, into a corn-based bait and conducted a series of experiments to determine its toxicity, leaching rate, and species-specificity. Our results showed that ANT-A was lethal to carp at doses ≥ 4 mg/kg and that the amount of ANT-A that leached out of the bait in 72 h was not lethal to carp or bluegill (Lepomis macrochirus). Species-specificity trials were conducted in 227 L tanks, in which carp were stocked with three native species representing families that occur sympatrically with carp in our study region: the fathead minnow (Pimephales promelas), yellow perch (Perca flavescens) and bluegill. These trials showed high mortality of carp (46%) and fathead minnows (76%) but no significant mortality of perch or bluegill. Finally, a pond study, which used the same species composition except for fathead minnows, resulted in 37% morality among adult carp and no mortality among perch or bluegill. Our results suggest that corn-based bait that contains ANT-A could be used to selectively control carp in ecosystems dominated by percids or centrarchids, such as lakes across the Great Plains ecoregion of North America, where carp are especially problematic.

Water-quality sampling plan for evaluating the distribution of bigheaded carps in the Illinois Waterway

USGS Publications Warehouse

Duncker, James J.; Terrio, Paul J.

2017-02-27

The two nonnative invasive bigheaded carp species (bighead carp Hypophthalmichthys nobilis and silver carp H. molitrix) that were accidentally released in the 1970s have spread widely into the rivers and waterways of the Mississippi River Basin. First detected in the lower reaches of the Illinois Waterway (IWW, the combined Illinois River-Des Plaines River-Chicago Area Waterway System) in the 1990s, bighead and silver carps moved quickly upstream, approaching the Chicago Area Waterway System. The potential of substantial negative ecological and economic impact to the Great Lakes from the presence of these species is a concern. However, since 2006, the population front of bigheaded carps has remained in the vicinity of Joliet, Illinois, near river mile 278. This reach of the IWW is characterized by stark changes in habitat, water quality, and food resources as the waterway transitions from a primarily agricultural landscape to a metropolitan and industrial canal system. This report describes a 2015 plan for sampling the IWW to establish water-quality conditions that might be contributing to the apparent stalling of the population front of bigheaded carps in this reach. A detailed description of the study plan, Lagrangian-style sampling approach, selected analytes, sampling methods and protocols are provided. Hydrographs from streamflow-gaging stations show IWW conditions during the 2015 sampling runs.

Complete mitochondrial genome of Yangtze River wild common carp (Cyprinus carpio haematopterus) and Russian scattered scale mirror carp (Cyprinus carpio carpio).

PubMed

Hu, Guang Fu; Liu, Xiang Jiang; Zou, Gui Wei; Li, Zhong; Liang, Hong-Wei; Hu, Shao-Na

2016-01-01

We sequenced the complete mitogenomes of (Cyprinus carpio haematopterus) and Russian scattered scale mirror carp (Cyprinus carpio carpio). Comparison of these two mitogenomes revealed that the mitogenomes of these two common carp strains were remarkably similar in genome length, gene order and content, and AT content. There were only 55 bp variations in 16,581 nucleotides. About 1 bp variation was located in rRNAs, 2 bp in tRNAs, 9 bp in the control region and 43 bp in protein-coding genes. Furthermore, forty-three variable nucleotides in the protein-coding genes of the two strains led to four variable amino acids, which were located in the ND2, ATPase 6, ND5 and ND6 genes, respectively.

33 CFR 80.170 - Sandy Hook, NJ to Tom's River, NJ.

Code of Federal Regulations, 2010 CFR

2010-07-01

... 33 Navigation and Navigable Waters 1 2010-07-01 2010-07-01 false Sandy Hook, NJ to Tom's River, NJ. 80.170 Section 80.170 Navigation and Navigable Waters COAST GUARD, DEPARTMENT OF HOMELAND SECURITY INTERNATIONAL NAVIGATION RULES COLREGS DEMARCATION LINES Atlantic Coast § 80.170 Sandy Hook, NJ to Tom's River...

Changes of pH and peroxide value in carp (Cyprinus carpio) cuts packaged in modified atmosphere

NASA Astrophysics Data System (ADS)

Milijašević, M.; Babić Milijašević, J.; Đinović-Stojanović, J.; Vesković Moračanin, S.; Lilić, S.

2017-09-01

The aim of our research was to examine the influence of packaging in modified atmosphere on the pH and peroxide value in muscle of common carp (Cyprinus carpio), as well as to determine the most suitable gas mixtures for packing of that freshwater species. Three sample groups of carp cuts were investigated. One group of carp cuts was placed on top of flaked ice placed in polystyrene boxes. Two other groups were packaged in modified atmosphere with different gas ratios: 80%O2+20%CO2 (MAP 1) and 90%CO2+10%N2 (MAP 2). All carp cuts were stored in the same conditions at 3±0.5°C, and on 1, 3, 5, 7, 9, 11, 13, 15. and 17 days of storage, chemical testing was performed. The results obtained indicate that the packaging of common carp under 90%CO2+10%N2 slowed proteolytic reaction as well as secondary lipid oxidation.

Swimming Performance of Bighead Carp and Silver Carp: Methodology, Metrics, and Management Applications

DTIC Science & Technology

2012-08-01

upstream. “Accelerated Water Velocity” is under consideration as a control for aquatic nuisance species in the Great Lakes-Mississippi River Interbasin...2009. Oxygen consumption rates for bighead and silver carp in relation to life-stage and water temperature. J. Freshwater Ecology 24(4): 535-543...19.5 SD) are displaced by water velocities averaging only 25 cm/s + 3.8 SD (Layher and Ralston 1997). High activity levels of two enzymes

In vitro biomechanical comparison of pedicle screws, sublaminar hooks, and sublaminar cables.

PubMed

Hitchon, Patrick W; Brenton, Matthew D; Black, Andrew G; From, Aaron; Harrod, Jeremy S; Barry, Christopher; Serhan, Hassan; Torner, James C

2003-07-01

Three types of posterior thoracolumbar implants are in use today: pedicle screws, sublaminar titaniumcables, and sublaminar hooks. The authors conducted a biomechanical comparison of these three implants in human cadaveric spines. Spine specimens (T5-12) were harvested, radiographically assessed for fractures or metastases, and their bone mineral density (BMD) was measured. Individual vertebrae were disarticulated and fitted with either pedicle screws, sublaminar cables, or bilateral claw hooks. The longitudinal component of each construct consisted of bilateral 10-cm rods connected with two cross-connectors. The vertebral body was embedded in cement, and the rods were affixed to a ball-and-socket apparatus for the application of a distraction force. The authors analyzed 1) 20 vertebrae implanted with screws; 2) 20 with hooks, and 3) 20 with cables. The maximum pullout (MPO) forces prior to failure (mean +/- standard deviation) for the screw, hook, and cable implants were 972 +/- 330, 802 +/- 356, and 654 +/- 248 N, respectively (p = 0.0375). Cables allowed significantly greater displacement (6.80 +/- 3.95 mm) prior to reaching the MPO force than hooks (3.73 +/- 1.42 mm) and screws (4.42 +/- 2.15 mm [p = 0.0108]). Eleven screw-implanted vertebrae failed because of screw pullout. All hook-and-cable-implanted vertebrae failed because of pedicle, middle column, or laminar fracture. These findings suggest that screws possess the greatest pullout strength of the three fixation systems. Sublaminar cables are the least rigid of the three. When screw failure occurred, the mechanism was generally screw back-out, without vertebral fractures.

Comparison of PCR methods for the detection of genetic variants of carp edema virus.

PubMed

Adamek, Mikolaj; Matras, Marek; Jung-Schroers, Verena; Teitge, Felix; Heling, Max; Bergmann, Sven M; Reichert, Michal; Way, Keith; Stone, David M; Steinhagen, Dieter

2017-09-20

The infection of common carp and its ornamental variety, koi, with the carp edema virus (CEV) is often associated with the occurrence of a clinical disease called 'koi sleepy disease'. The disease may lead to high mortality in both koi and common carp populations. To prevent further spread of the infection and the disease, a reliable detection method for this virus is required. However, the high genetic variability of the CEV p4a gene used for PCR-based diagnostics could be a serious obstacle for successful and reliable detection of virus infection in field samples. By analysing 39 field samples from different geographical origins obtained from koi and farmed carp and from all 3 genogroups of CEV, using several recently available PCR protocols, we investigated which of the protocols would allow the detection of CEV from all known genogroups present in samples from Central European carp or koi populations. The comparison of 5 different PCR protocols showed that the PCR assays (both end-point and quantitative) developed in the Centre for Environment, Fisheries and Aquaculture Science exhibited the highest analytical inclusivity and diagnostic sensitivity. Currently, this makes them the most suitable protocols for detecting viruses from all known CEV genogroups.

Structure of the bacterial flagellar hook and implication for the molecular universal joint mechanism.

PubMed

Samatey, Fadel A; Matsunami, Hideyuki; Imada, Katsumi; Nagashima, Shigehiro; Shaikh, Tanvir R; Thomas, Dennis R; Chen, James Z; Derosier, David J; Kitao, Akio; Namba, Keiichi

2004-10-28

The bacterial flagellum is a motile organelle, and the flagellar hook is a short, highly curved tubular structure that connects the flagellar motor to the long filament acting as a helical propeller. The hook is made of about 120 copies of a single protein, FlgE, and its function as a nano-sized universal joint is essential for dynamic and efficient bacterial motility and taxis. It transmits the motor torque to the helical propeller over a wide range of its orientation for swimming and tumbling. Here we report a partial atomic model of the hook obtained by X-ray crystallography of FlgE31, a major proteolytic fragment of FlgE lacking unfolded terminal regions, and by electron cryomicroscopy and three-dimensional helical image reconstruction of the hook. The model reveals the intricate molecular interactions and a plausible switching mechanism for the hook to be flexible in bending but rigid against twisting for its universal joint function.

Polymorphism of transferrin in carp (Cyprinus carpio L.): genetic determination, isolation, and partial characterization.

PubMed

Valenta, M; Stratil, A; Slechtová, V; Kálal, L; Slechta, V

1976-02-01

Seven transferrin variants (A,B,C,D,E,F, and G) have been found in carp sera (Cyprinus carpio L.). Genetic analysis involves five variants and agrees with the hypothesis of simple codominant autosomal inheritance at one transferrin (Tf) locus in spite of the fact that the carp is a tetraploid in relation to other species of the same family. Carp populations from three regions were studied which differed in gene frequencies. Individual populations were in Hardy-Weinberg equilibrium. The polymorphism of carp transferrins can be used for the identification of offspring of single parent pairs, stocked in one pond. Transferrins have been isolated and characterized. Homozygous phenotypes comprised four iron-binding components differing in electrophoretic mobility. This heterogeneity is not caused by sialic acid, which is absent. Amino acid composition, content of hexoses (1 mole/mole of protein) and hexosamines (1 mole/mole of protein), molecualr weight (70,000), and the isoelectric point (5.0) have been determined. No N-terminal amino acid could be detected.

Comparative mapping for bighead carp (Aristichthys nobilis) against model and non-model fishes provides insights into the genomic evolution of cyprinids.

PubMed

Zhu, Chuankun; Tong, Jingou; Yu, Xiaomu; Guo, Wenjie

2015-08-01

Comparative mapping provides an efficient method to connect genomes of non-model and model fishes. In this study, we used flanking sequences of the 659 microsatellites on a genetic map of bighead carp (Aristichthys nobilis) to comprehensively study syntenic relationships between bighead carp and nine model and non-model fishes. Of the five model and two food fishes with whole genome data, Cyprinus carpio showed the highest rate of positive BLAST hits (95.3 %) with bighead carp map, followed by Danio rerio (70.9 %), Oreochromis niloticus (21.7 %), Tetraodon nigroviridis (6.4 %), Gasterosteus aculeatus (5.2 %), Oryzias latipes (4.7 %) and Fugu rubripes (3.5 %). Chromosomal syntenic analyses showed that inversion was the basic chromosomal rearrangement during genomic evolution of cyprinids, and the extent of inversions and translocations was found to be positively correlated with evolutionary relationships among fishes studied. Among the five investigated cyprinids, linkage groups (LGs) of bighead carp, Hypophthalmichthys molitrix and Ctenopharyngodon idella exhibited a one-to-one relationship. Besides, LG 9 of bighead carp and homologous LGs of silver carp and grass carp all corresponded to the chromosomes 10 and 22 of zebrafish, suggesting that chromosomal fission may have occurred in the ancestor of zebrafish. On the other hand, LGs of bighead carp and common carp showed an approximate one-to-two relationship with extensive translocations, confirming the occurrence of a 4th whole genome duplication in common carp. This study provides insights into the understanding of genome evolution among cyprinids and would aid in transferring positional and functional information of genes from model fish like zebrafish to non-model fish like bighead carp.

Carp edema virus/Koi sleepy disease: an emerging disease in Central-East Europe.

PubMed

Lewisch, E; Gorgoglione, B; Way, K; El-Matbouli, M

2015-02-01

Koi sleepy disease (KSD), also known as carp edema virus (CEV), was first reported from juvenile colour carp in Japan in the 1970s. Recently, this pox virus was detected in several European countries, including Germany, France and the Netherlands. In England, in addition to colour carp, outbreaks in common carp are reported. KSD/CEV is an emerging infectious disease characterized by a typical sleepy behaviour, enophthalmia, generalized oedematous condition and gill necrosis, leading to hypoxia. High mortality, of up to 80-100%, is seen in juvenile koi collected from infected ponds. In Austria, this disease had not been detected until now. In spring 2014, diagnostic work revealed the disease in two unrelated cases. In one instance, a pond with adult koi was affected; in the other, the disease was diagnosed in adult common carp recently imported from the Czech Republic. A survey was carried out on recent cases (2013/2014), chosen from those with similar anamnestic and physical examination findings, revealing a total of 5/22 cases positive for KSD/CEV. In this study, two paradigmatic cases are presented in detail. Results together with molecular evidence shaped the pattern of the first diagnosis of KSD/CEV in fish from Austrian ponds. In the light of the positive cases detected from archived material, and the spread of the disease through live stock, imported from a neighbouring country, the need for epidemiological investigations in Austria and surrounding countries is emphasized. © 2014 Blackwell Verlag GmbH.

Molecular cloning, characterization and expression analysis of coagulation factor VII gene in grass carp (Ctenopharyngodon idella).

PubMed

Liu, Qiaolin; Xu, Baohong; Xiao, Tiaoyi; Su, Jianming; Zhong, Lei

2013-08-01

Coagulation factor VII has been studied in several species but, to date, not in grass carp (Ctenopharyngodon idella), a commercially important freshwater fish found in China. In this study, the full-length cDNA of grass carp coagulation factor VII (GcCFVII) was cloned using a RACE-Ready cDNA Kit, grass carp were challenged with a hemorrhagic virus, and temporal expression profiles of GcCFVII in the thymus, gills, liver, spleen, and head kidney were examined at 0 h, 24 h, 48 h, 72 h, 96 h, and 138 h using fluorescence quantitative PCR. The results showed the 1480 bp GcCFVII to contain three conservative motifs: Gla, EGF-CA, and Tryp-SPc, similar to other species. Phylogenetic analysis showed the evolution of GcCFVII gene to be consistent with the evolution of the species. After viral challenge, GcCFVII expression in five tissues of grass carp showed different patterns of fluctuation. These results provide a solid basis for further investigation of GcCFVII and its relationship with grass carp hemorrhage. Copyright © 2013 Elsevier Ltd. All rights reserved.

Crafting the microworld: how Robert Hooke constructed knowledge about small things

PubMed Central

Lawson, Ian

2016-01-01

This paper investigates the way in which Robert Hooke constructed his microscopical observations. His Micrographia is justifiably famous for its detailed engravings, which communicated Hooke's observations of tiny nature to his readers, but less attention has been paid to how he went about making the observations themselves. In this paper I explore the relationship between the materiality of his instrument and the epistemic images he produced. Behind the pictures lies an array of hidden materials, and the craft knowledge it took to manipulate them. By investigating the often counter-theoretical and conflicting practices of his ingenious microscope use, I demonstrate the way in which Hooke crafted the microworld for his readers, giving insight into how early modern microscopy was understood by its practitioners and audience. PMID:27017680

CRAFTING THE MICROWORLD: HOW ROBERT HOOKE CONSTRUCTED KNOWLEDGE ABOUT SMALL THINGS.

PubMed

Lawson, Ian

2016-03-20

This paper investigates the way in which Robert Hooke constructed his microscopical observations. His Micrographia is justifiably famous for its detailed engravings, which communicated Hooke's observations of tiny nature to his readers, but less attention has been paid to how he went about making the observations themselves. In this paper I explore the relationship between the materiality of his instrument and the epistemic images he produced. Behind the pictures lies an array of hidden materials, and the craft knowledge it took to manipulate them. By investigating the often counter-theoretical and conflicting practices of his ingenious microscope use, I demonstrate the way in which Hooke crafted the microworld for his readers, giving insight into how early modern microscopy was understood by its practitioners and audience.

Influence of hook position on phototropic and gravitropic curvature by etiolated hypocotyls of Arabidopsis thaliana

NASA Technical Reports Server (NTRS)

Khurana, J. P.; Best, T. R.; Poff, K. L.

1989-01-01

Phototropic and gravitropic curvature by hypocotyls of Arabidopsis thaliana is minimal when the side of the hook with the cotyledons attached is positioned toward the direction of tropistic curvature, and maximal when that side of the hook is positioned away from the direction of tropistic curvature. Based on these data, it is proposed that the position of the hook with attached cotyledons affects curvature and not stimulus perception. A randomly oriented population of plants exhibited considerable heterogeneity in tropistic curvature. This heterogeneity arises at least in part from the dependence of curvature on the position of the hook.

Influence of hook position on phototropic and gravitropic curvature by etiolated hypocotyls of Arabidopsis thaliana.

PubMed

Khurana, J P; Best, T R; Poff, K L

1989-01-01

Phototropic and gravitropic curvature by hypocotyls of Arabidopsis thaliana is minimal when the side of the hook with the cotyledons attached is positioned toward the direction of tropistic curvature, and maximal when that side of the hook is positioned away from the direction of tropistic curvature. Based on these data, it is proposed that the position of the hook with attached cotyledons affects curvature and not stimulus perception. A randomly oriented population of plants exhibited considerable heterogeneity in tropistic curvature. This heterogeneity arises at least in part from the dependence of curvature on the position of the hook.

Binational ecological risk assessment of bigheaded carps (Hypophthalmichthys spp.) for the Great Lakes Basin.

USGS Publications Warehouse

Cudmore, Becky; Mandrak, Nicholas E.; Dettmers, John M.; Chapman, Duane C.; Kolar, Cynthia S.

2012-01-01

Bigheaded carps (Bighead and Silver carps) are considered a potential threat to the Great Lakes basin. A binational ecological risk assessment was conducted to provide scientifically defensible advice for managers and decision-makers in Canada and the United States. This risk assessment looked at the likelihood of arrival, survival, establishment, and spread of bigheaded carps to obtain an overall probability of introduction. Arrival routes assessed were physical connections and human-mediated releases. The risk assessment ranked physical connections (specifically the Chicago Area Waterway System) as the most likely route for arrival into the Great Lakes basin. Results of the risk assessment show that there is enough food and habitat for bigheaded carp survival in the Great Lakes, especially in Lake Erie and productive embayments in the other lakes. Analyses of tributaries around the Canadian Great Lakes and the American waters of Lake Erie indicate that there are many suitable tributaries for bigheaded carp spawning. Should bigheaded carps establish in the Great Lakes, their spread would not likely be limited and several ecological consequences can be expected to occur. These consequences include competition for planktonic food leading to reduced growth rates, recruitment and abundance of planktivores. Subsequently this would lead to reduced stocks of piscivores and abundance of fishes with pelagic, early life stages. Overall risk is highest for lakes Michigan, Huron, and Erie, followed by Lake Ontario then Lake Superior. To avoid the trajectory of the invasion process and prevent or minimize anticipated consequences, it is important to continue to focus efforts on reducing the probability of introduction of these species at either the arrival, survival, establishment, or spread stage (depending on location).

Surgical treatment of acute acromioclavicular joint dislocations: hook plate versus minimally invasive reconstruction.

PubMed

Metzlaff, S; Rosslenbroich, S; Forkel, P H; Schliemann, B; Arshad, H; Raschke, M; Petersen, W

2016-06-01

This study was performed to compare the clinical results of a minimally invasive technique for acute acromioclavicular (AC) joint dislocation repair with the traditional hook plate fixation. Forty-four patients with an acute (within 2 weeks after trauma) complete AC joint separation (35 male, nine female; median age 36.2 years, range 18-56) underwent surgical repair with either a minimally invasive AC joint repair or a conventional hook plate. Functional outcome was evaluated using the Constant-Murley Score (CMS), the TAFT score and the AC joint instability score (ACJI). Radiographic evaluation was performed with bilateral anterior-posterior (a.p.) stress and Alexander views. All patients were available after a median follow-up of 32 months (range 24-51). There were no significant differences in the mean CMS, Taft score and the ACJI between the two groups. The radiological assessment revealed no significant difference in the coracoclavicular distance. In both groups, a slight loss of reduction was observed. Periarticular ossification was seen in 11 patients of the minimally invasive AC joint repair and eight patients of the hook plate group but this did not affect the final outcome. Hook plates were removed after a median interval of 11.9 weeks (range 10-13). Good clinical results can be achieved with both minimally invasive AC joint repair and hook plate fixation. However, in the hook plate group a second operation is mandatory for plate removal. III.

Hook Region Represented in a Cochlear Model

NASA Astrophysics Data System (ADS)

Steele, Charles R.; Kim, Namkeun; Puria, Sunil

2009-02-01

The present interest is in discontinuities. Particularly the geometry of the hook region, with the flexible round window nearly parallel with the basilar membrane, is not represented by a standard box model, in which both stapes and round window are placed at the end. A better model represents the round window by a soft membrane in the wall of scala tympani, with the end closed. This complicates the analysis considerably. Features are that the significant compression wave, i.e., the fast wave, is of negligible magnitude in this region, and that significant evanescent waves occur because of the discontinuities at the beginning and end of the simulated round window. The effect of this on both high frequency, with maximum basilar membrane response in the hook region, and lower frequencies are determined.

[Comparison between J-hook and micro-implant anchorage in the treatment of patients with bimaxillary protrusion].

PubMed

Chen, Wen-Jing; Li, Qing-Yi; Gong, Ai-Xiu; Hu, Fang; Gu, Yong-Jia

2008-02-01

To compare the difference between J-hook and micro-implant anchorage in the treatment of patient with bimaxillary protrusion. Thirty patients with bimaxillary protrusion were divided into two groups (J-hook and micro-implant groups) and treated with MBT appliance. Four first premolars were extracted in all patients. Cephalometric analyses were carried out before and after treatment. In J-hook group and micro-implant group,computerized cephalometric analysis revealed that before treatment U6C-PP was (12.4 +/- 0.2) mm and (12.5 +/- 0.1) mm, respectively,and after treatment U6C-PP was (12.6 +/- 0.1) mm and (12.8 +/- 0.1) mm,respectively. The difference between J-hook group and microimplant group was significant (P < 0.01). The other differences of cephalometric analyses between J-hook group and micro-implant group was not significant. Both J-hook and micro-implant could provide adequate anchorage in the treatment of patients with bimaxillary protrusion.

Ferromagnetic resonance in the ethmoid bones of salmon and silver carp

NASA Astrophysics Data System (ADS)

Gorobets, Svitlana; Gorobets, Oksana; Golub, Volodymyr; Gromnadska, Marina

2017-10-01

The detection of biogenic magnetic nanoparticles (BMN) with different magnetic properties in biological material was done using magnetic resonance (MR) spectroscopy. MR spectra of biological material of ethmoid bone of salmon (containing ferritin and BMN), bacteria E. coli K13 (containing ferritin and without BMN), yeast S. cerevisiae (without ferritin or BMN) and ethmoid bone of silver carp (containing ferritin and not investigated for the presence of BMN) were investigated. The analysis of MR spectra shows that S. cerevisiae cells produce much lower signal MR than samples of ethmoid bones of salmon and silver carp which is confirming conclusions about the presence of BMN and ferritin in the ethmoid bones of fishes. The narrow MR linewidth indicates that the magnetic particles in the ethmoid bones of salmon and silver carp are in monodisperse state. The presence of a broad line and the absence of a narrow peak in MR spectrum of E. coli K13 cells are typical for ferritin.

Spatial and temporal influences on the physiological condition of invasive silver carp.

PubMed

Liss, Stephanie A; Sass, Greg G; Suski, Cory D

2013-01-01

We quantified nutritional and stress parameters (alkaline phosphatase, cholesterol, protein, triglycerides, cortisol, and glucose) in invasive silver carp (Hypophthalmichthys molitrix) inhabiting four large rivers throughout three distinct time periods in the Midwestern USA. Examining the basic biology and ecology of an invasive species is crucial to gain an understanding of the interaction between an organism and its environment. Analysis of the physiological condition of wild-caught silver carp across broad spatial and temporal scales is essential because stress and nutritional parameters can link individuals to their habitats and vary among populations across environments. During each time period, we collected blood samples from individual silver carp in the Illinois River and portions of the Mississippi, Ohio, and Wabash rivers in Illinois. We tested for relationships between silver carp nutrition and stress across rivers, reaches within rivers, and time periods. Principal component analyses separated physiological parameters into a stress component (cortisol and glucose) and two nutritional components representative of short-term feeding (alkaline phosphatase, protein, and triglycerides) and body energy reserves (cholesterol and protein). Akaike's information criterion suggested that time period had the greatest influence on stress. Stress levels were consistent in all four rivers, and declined across time periods. Akaike's information criterion also suggested that interactions of time period and river had the greatest influence on short-term feeding and body energy reserves. There was no specific pattern across time periods within each river, nor was there a pattern across rivers. Our results provide a better understanding of nutritional and stress conditions in invasive silver carp across a broad landscape and temporal scale, with implications for managing and predicting the spread of this species.

Tissue-Specific Fatty Acids Response to Different Diets in Common Carp (Cyprinus carpio L.)

PubMed Central

Böhm, Markus; Schultz, Sebastian; Koussoroplis, Apostolos-Manuel; Kainz, Martin J.

2014-01-01

Fish depend on dietary fatty acids (FA) to support their physiological condition and health. Exploring the FA distribution in common carp (Cyprinus carpio), one of the world's most consumed freshwater fish, is important to understand how and where FA of different sources are allocated. We investigated diet effects on the composition of polar and neutral lipid fatty acids (PLFA and NLFA, respectively) in eight different tissues (dorsal and ventral muscle, heart, kidney, intestine, eyes, liver and adipose tissue) of common carp. Two-year old carp were exposed to three diet sources (i.e., zooplankton, zooplankton plus supplementary feeds containing vegetable, VO, or fish oil, FO) with different FA composition. The PLFA and NLFA response was clearly tissue-specific after 210 days of feeding on different diets. PLFA were generally rich in omega-3 polyunsaturated FA and only marginally influenced by dietary FA, whereas the NLFA composition strongly reflected dietary FA profiles. However, the NLFA composition in carp tissues varied considerably at low NLFA mass ratios, suggesting that carp is able to regulate the NLFA composition and thus FA quality in its tissues when NLFA contents are low. Finally, this study shows that FO were 3X more retained than VO as NLFA particularly in muscle tissues, indicating that higher nutritional quality feeds are selectively allocated into tissues and thus available for human consumption. PMID:24733499

Native freshwater species get out of the way: Prussian carp (Carassius gibelio) impacts both fish and benthic invertebrate communities in North America.

PubMed

Ruppert, Jonathan L W; Docherty, Cassandra; Neufeld, Kenton; Hamilton, Kyle; MacPherson, Laura; Poesch, Mark S

2017-10-01

Prussian carp ( Carassius gibelio ) are one of the most noxious non-native species in Eurasia. Recently, Prussian carp, a non-native freshwater fish species, were genetically confirmed in Alberta, Canada and have been rapidly expanding their range in North America since establishment. Given their rapid range expansion, there is an increasing need to determine how Prussian carp may impact native species. We assessed the severity of the Prussian carp invasion by (i) determining their impact on fish communities, (ii) assessing their impact on benthic invertebrate communities, (iii) evaluating if Prussian carp alter abiotic conditions, and (iv) identifying where we find higher abundances of Prussian carp. When Prussian carp were established, we found significant changes to the fish community. Correspondingly, the degree of impact to benthic invertebrate communities was related to the stage of invasion (none, early or recent), where changes in fish communities were significantly concordant with changes in benthic invertebrate communities. Finally, we found that higher abundances of Prussian carp were significantly associated with lower abundances of a majority of native fish species. Altogether, using three lines of evidence, we determine that Prussian carp can have wide-ranging impacts on freshwater ecosystems in North America, pressing the need for management intervention.

Native freshwater species get out of the way: Prussian carp (Carassius gibelio) impacts both fish and benthic invertebrate communities in North America

PubMed Central

Ruppert, Jonathan L. W.; Docherty, Cassandra; Neufeld, Kenton; Hamilton, Kyle; MacPherson, Laura

2017-01-01

Prussian carp (Carassius gibelio) are one of the most noxious non-native species in Eurasia. Recently, Prussian carp, a non-native freshwater fish species, were genetically confirmed in Alberta, Canada and have been rapidly expanding their range in North America since establishment. Given their rapid range expansion, there is an increasing need to determine how Prussian carp may impact native species. We assessed the severity of the Prussian carp invasion by (i) determining their impact on fish communities, (ii) assessing their impact on benthic invertebrate communities, (iii) evaluating if Prussian carp alter abiotic conditions, and (iv) identifying where we find higher abundances of Prussian carp. When Prussian carp were established, we found significant changes to the fish community. Correspondingly, the degree of impact to benthic invertebrate communities was related to the stage of invasion (none, early or recent), where changes in fish communities were significantly concordant with changes in benthic invertebrate communities. Finally, we found that higher abundances of Prussian carp were significantly associated with lower abundances of a majority of native fish species. Altogether, using three lines of evidence, we determine that Prussian carp can have wide-ranging impacts on freshwater ecosystems in North America, pressing the need for management intervention. PMID:29134062

Seasonal distribution, aggregation, and habitat selection of common carp in Clear Lake, Iowa

USGS Publications Warehouse

Penne, C.R.; Pierce, C.L.

2008-01-01

The common carp Cyprinus carpio is widely distributed and frequently considered a nuisance species outside its native range. Common carp are abundant in Clear Lake, Iowa, where their presence is both a symptom of degradation and an impediment to improving water quality and the sport fishery. We used radiotelemetry to quantify seasonal distribution, aggregation, and habitat selection of adult and subadult common carp in Clear Lake during 2005-2006 in an effort to guide future control strategies. Over a 22-month period, we recorded 1,951 locations of 54 adults and 60 subadults implanted with radio transmitters. Adults demonstrated a clear tendency to aggregate in an offshore area during the late fall and winter and in shallow, vegetated areas before and during spring spawning. Late-fall and winter aggregations were estimated to include a larger percentage of the tracked adults than spring aggregations. Subadults aggregated in shallow, vegetated areas during the spring and early summer. Our study, when considered in combination with previous research, suggests repeatable patterns of distribution, aggregation, and habitat selection that should facilitate common carp reduction programs in Clear Lake and similar systems. ?? Copyright by the American Fisheries Society 2008.

Investigation for zoonotic disease pathogens (Aeromonas hydrophila, Pseudomonas fluorescens, Streptococcus iniae) seen in carp farms in Duhok region of Northern Iraq by molecular methods

NASA Astrophysics Data System (ADS)

Mohammed, Kamiran Abdulrahman; Arabacı, Muhammed; Önalan, Şükrü

2017-04-01

The aim of this study was to determine the zoonotic bacteria in carp farms in Duhok region of the Northern Iraq. Carp is the main fish species cultured in the Duhok region. The most common zoonotic bacteria generally seen in carp farms are Aeromonas hydrophila, Pseudomonas fluorescens and Streptococcus iniae. Samples were collected from 20 carp farms in the Duhok Region of the Northern Iraq. Six carp samples were collected from each carp farm. Head kidney tissue samples and intestine tissue samples were collected from each carp sample. Than head kidney and intestine tissue samples were pooled. The total bacterial DNA extraction from the pooled each 20 head kidney tissue samples and pooled each 20 intestinal tissue samples. Primers for pathogens were originally designed from 16S Ribosomal gene region. Zoonotic bacteria were scanned in all tissue samples by absent / present analysis in the RT-PCR. After RT-PCR, Capillary gel electrophoresis bands were used for the confirmation of the size of amplicon which was planned during primer designing stage. As a result, one sample was positive in respect to Aeromonas hydrophila, from intestine and one carp farm was positive in respect to Pseudomonas fluorescens from intestine and two carp farms were positive in respect to Streptococcus iniae. Totally 17 of 20 carp farms were negative in respect to the zoonotic bacteria. In conclusion the zoonotic bacteria were very low (15 %) in carp farms from the Duhok Region in the Northern Iraq. Only in one Carp farms, both Aeromonas hydrophila and Pseudomonas fluorescens were positive. Also Streptococcus inia were positive in two carp farms.

Cyprinid herpesvirus-3 (CyHV-3) disturbs osmotic balance in carp (Cyprinus carpio L.)--A potential cause of mortality.

PubMed

Negenborn, J; van der Marel, M C; Ganter, M; Steinhagen, D

2015-06-12

Cyprinid herpesvirus-3 (CyHV-3) causes a fatal disease in carp (Cyprinus carpio) and its ornamental koi varieties which seriously affects production and trade of this fish species globally. Up to now, the pathophysiology of this disease remains unclear. Affected individuals develop most prominent lesions in gills, skin and kidney, in tissues which are involved in the osmotic regulation of freshwater teleosts. Therefore, here serum and urine electrolyte levels were examined during the course of an experimental infection of carp with CyHV-3. In infected carp an interstitial nephritis with a progressive deterioration of nephric tubules developed, which was paralleled by elevated electrolyte losses, mainly Na(+) in the urine. The urine/plasma ratio for Na(+) increased from 0.03 in uninfected carp to 0.43-0.83 in carp under CyHV-3 infection, while concentration of divalent ions were not significantly changed. These electrolyte losses could not be compensated since plasma osmolality and Na(+) concentration dropped significantly in CyHV-3 infected carp. This was most probably caused by the progressive deterioration of the branchial epithelium, which in teleosts plays a prominent role in osmoregulation, and which was seen concomitantly with decreasing electrolyte levels in the serum of carp under CyHV-3 infection. Immediately after infection with CyHV-3, by day 2 post exposure, affected carp showed severe anaemia and prominent leucocytosis indicating the development of an acute inflammation, which could intensify the observed hydro-mineral imbalances. The data presented here show that an infection with CyHV-3 induces an acute inflammation and a severe dysfunction of osmoregulation in affected carp or koi, which may lead to death in particular in the case of acute disease progression. Copyright © 2015 Elsevier B.V. All rights reserved.

Performance Outcomes After Hook of Hamate Fractures in Major League Baseball Players.

PubMed

Guss, Michael S; Begly, John P; Ramme, Austin J; Taormina, David P; Rettig, Michael E; Capo, John T

2017-07-17

Major League Baseball (MLB) players are at risk of hook of hamate fractures. There is a paucity of data assessing the effect of a hook of hamate fracture on MLB players' future athletic performance. To determine if MLB players who sustain hook of hamate fractures demonstrate decreased performance upon return to competition when compared with their performance before injury and that of their control-matched peers. Retrospective Case-Control Design. Retrospective Database Study. 18 MLB players who sustained hook of hamate fractures. Data for 18 MLB players with hook of hamate fractures incurred over 26 seasons (1989 to 2014) were obtained from injury reports, press releases, and player profiles ( www.mlb.com and www.baseballreference.com ). Player age, position, number of years in the league, mechanism of injury and treatment were recorded. Individual season statistics for the two seasons immediately prior to injury and the two seasons after injury for the main performance variable - wins above replacement (WAR) were obtained. Eighteen controls matched by player position, age, and performance statistics were identified. A performance comparison of the cohorts was performed. Post-injury performance compared to pre-injury performance and matched-controls. Mean age at the time of injury was 25.1 years with a mean of 4.4 seasons of MLB experience prior to injury. All injuries were sustained to their nondominant batting hand. All players underwent operative intervention. There was no significant change in WAR or ISO when pre-injury and post-injury performance was compared. When compared with matched-controls, no significant decline in performance in WAR the first season and second season after injury was found. MLB players sustaining hook of hamate fractures can reasonably expect to return to their pre-injury performance levels following operative treatment.

A biomechanical analysis of the self-retaining pedicle hook device in posterior spinal fixation

PubMed Central

van Laar, Wilbert; Meester, Rinse J.; Smit, Theo H.

2007-01-01

Regular hooks lack initial fixation to the spine during spinal deformity surgery. This runs the risk of posterior hook dislodgement during manipulation and correction of the spinal deformity, that may lead to loss of correction, hook migration, and post-operative junctional kyphosis. To prevent hook dislodgement during surgery, a self-retaining pedicle hook device (SPHD) is available that is made up of two counter-positioned hooks forming a monoblock posterior claw device. The initial segmental posterior fixation strength of a SPHD, however, is unknown. A biomechanical pull-out study of posterior segmental spinal fixation in a cadaver vertebral model was designed to investigate the axial pull-out strength for a SPHD, and compared to the pull-out strength of a pedicle screw. Ten porcine lumbar vertebral bodies were instrumented in pairs with two different instrumentation constructs after measuring the bone mineral density of each individual vertebra. The instrumentation constructs were extracted employing a material testing system using axial forces. The maximum pull-out forces were recorded at the time of the construct failure. Failure of the SPHD appeared in rotation and lateral displacement, without fracturing of the posterior structures. The average pull-out strength of the SPHD was 236 N versus 1,047 N in the pedicle screws (P < 0.001). The pull-out strength of the pedicle screws showed greater correlation with the BMC compared to the SPHD (P < 0.005). The SPHD showed to provide a significant inferior segmental fixation to the posterior spine in comparison to pedicle screw fixation. Despite the beneficial characteristics of the monoblock claw construct in a SPHD, that decreases the risk of posterior hook dislodgement during surgery compared to regular hooks, the SPHD does not improve the pull-out strength in such a way that it may provide a biomechanically solid alternative to pedicle screw fixation in the posterior spine. PMID:17203270

Significant genetic differentiation between native and introduced silver carp (Hypophthalmichthys molitrix) inferred from mtDNA analysis

USGS Publications Warehouse

Li, S.-F.; Xu, J.-W.; Yang, Q.-L.; Wang, C.H.; Chapman, D.C.; Lu, G.

2011-01-01

Silver carp Hypophthalmichthys molitrix (Cyprinidae) is native to China and has been introduced to over 80 countries. The extent of genetic diversity in introduced silver carp and the genetic divergence between introduced and native populations remain largely unknown. In this study, 241 silver carp sampled from three major native rivers and two non-native rivers (Mississippi River and Danube River) were analyzed using nucleotide sequences of mitochondrial COI gene and D-loop region. A total of 73 haplotypes were observed, with no haplotype found common to all the five populations and eight haplotypes shared by two to four populations. As compared with introduced populations, all native populations possess both higher haplotype diversity and higher nucleotide diversity, presumably a result of the founder effect. Significant genetic differentiation was revealed between native and introduced populations as well as among five sampled populations, suggesting strong selection pressures might have occurred in introduced populations. Collectively, this study not only provides baseline information for sustainable use of silver carp in their native country (i.e., China), but also offers first-hand genetic data for the control of silver carp in countries (e.g., the United States) where they are considered invasive.

The hooked element in the pes of turtles (Testudines): a global approach to exploring primary and secondary homology

PubMed Central

Joyce, Walter G; Werneburg, Ingmar; Lyson, Tyler R

2013-01-01

The hooked element in the pes of turtles was historically identified by most palaeontologists and embryologists as a modified fifth metatarsal, and often used as evidence to unite turtles with other reptiles with a hooked element. Some recent embryological studies, however, revealed that this element might represent an enlarged fifth distal tarsal. We herein provide extensive new myological and developmental observations on the hooked element of turtles, and re-evaluate its primary and secondary homology using all available lines of evidence. Digital count and timing of development are uninformative. However, extensive myological, embryological and topological data are consistent with the hypothesis that the hooked element of turtles represents a fusion of the fifth distal tarsal with the fifth metatarsal, but that the fifth distal tarsal dominates the hooked element in pleurodiran turtles, whereas the fifth metatarsal dominates the hooked element of cryptodiran turtles. The term ‘ansulate bone’ is proposed to refer to hooked elements that result from the fusion of these two bones. The available phylogenetic and fossil data are currently insufficient to clarify the secondary homology of hooked elements within Reptilia. PMID:24102560

Gap compression/extension mechanism of bacterial flagellar hook as the molecular universal joint.

PubMed

Furuta, Tadaomi; Samatey, Fadel A; Matsunami, Hideyuki; Imada, Katsumi; Namba, Keiichi; Kitao, Akio

2007-03-01

Bacterial flagellar hook acts as a molecular universal joint, transmitting torque produced by the flagellar basal body, a rotary motor, to the flagellar filament. The hook forms polymorphic supercoil structures and can be considered as an assembly of 11 circularly arranged protofilaments. We investigated the molecular mechanism of the universal joint function of the hook by a approximately two-million-atom molecular dynamics simulation. On the inner side of the supercoil, protein subunits are highly packed along the protofilament and no gaps remain for further compression, whereas subunits are slightly separated and are hydrogen bonded through one layer of water molecules on the outer side. As for the intersubunit interactions between protofilaments, subunits are packed along the 6-start helix in a left-handed supercoil whereas they are highly packed along the 5-start helix in a right-handed supercoil. We conclude that the supercoiled structures of the hook in the left- and right-handed forms make maximal use of the gaps between subunits, which we call "gap compression/extension mechanism". Mutual sliding of subunits at the subunit interface accompanying rearrangements of intersubunit hydrogen bonds is interpreted as a mechanism to allow continuous structural change of the hook during flagellar rotation at low energy cost.

Talk about "hooking up": the influence of college student social networks on nonrelationship sex.

PubMed

Holman, Amanda; Sillars, Alan

2012-01-01

This research considers how communication within college student social networks may encourage high-risk sexual relationships. Students (n = 274) described sexual scripts for hooking up and reported on peer communication, sexual behavior, and sexual attitudes. Students described varied hookup scripts, expressed ambivalent attitudes, and reported moderate participation in hookups overall. However, the most common hookup script, suggesting high-risk sexual activity (i.e., unplanned, inebriated sex), was featured in most accounts of students who themselves participated in hookups. Students overestimated how often others were hooking up, and these estimates were especially inflated by students who frequently talked about hooking up with friends. Among students with strong ties to peers, frequent peer communication about sex predicted participation in hookups and favorable attitudes about hooking up. Peer approval also predicted hookup behavior and attitudes.

DNA Methylation of T1R1 Gene in the Vegetarian Adaptation of Grass Carp Ctenopharyngodon idella.

PubMed

Cai, Wenjing; He, Shan; Liang, Xu-Fang; Yuan, Xiaochen

2018-05-02

Although previous studies have indicated importance of taste receptors in food habits formation in mammals, little is known about those in fish. Grass carp is an excellent model for studying vegetarian adaptation, as it shows food habit transition from carnivore to herbivore. In the present study, pseudogenization or frameshift mutations of the umami receptors that hypothesized related to dietary switch in vertebrates, were not found in grass carp, suggesting other mechanisms for vegetarian adaptation in grass carp. T1R1 and T1R3 strongly responded to L-Arg and L-Lys, differing from those of zebrafish and medaka, contributing to high species specificity in amino acid preferences and diet selection of grass carp. After food habit transition of grass carp, DNA methylation levels were higher in CPG1 and CPG3 islands of upstream control region of T1R1 gene. Luciferase activity assay of upstream regulatory region of T1R1 (-2500-0 bp) without CPG1 or CPG3 indicated that CPG1 and CPG3 might be involved in transcriptional regulation of T1R1 gene. Subsequently, high DNA methylation decreased expression of T1R1 in intestinal tract. It could be a new mechanism to explain, at least partially, the vegetarian adaptation of grass carp by regulation of expression of umami receptor via epigenetic modification.

Genetic analysis shows that morphology alone cannot distinguish asian carp eggs from those of other cyprinid species

USGS Publications Warehouse

Larson, James H.; McCalla, S. Grace; Chapman, Duane C.; Rees, Christopher B.; Knights, Brent C.; Vallazza, Jon; George, Amy E.; Richardson, William B.; Amberg, Jon J.

2016-01-01

Fish eggs and embryos (hereafter collectively referred to as “eggs”) were collected in the upper Mississippi River main stem (~300 km upstream of previously reported spawning by invasive Asian carp) during summer 2013. Based on previously published morphological characteristics, the eggs were identified as belonging to Asian carp. A subsample of the eggs was subsequently analyzed by using molecular methods to determine species identity. Genetic identification using the cytochrome-c oxidase 1 gene was attempted for a total of 41 eggs. Due to the preservation technique used (formalin) and the resulting DNA degradation, sequences were recovered from only 17 individual eggs. In all 17 cases, cyprinids other than Asian carp (usually Notropis sp.) were identified as the most likely species. In previously published reports, a key characteristic that distinguished Asian carp eggs from those of other cyprinids was size: Asian carp eggs exhibited diameters ranging from 4.0 to 6.0 mm and were thought to be much larger than the otherwise similar eggs of native species. Eggs from endemic cyprinids were believed to rarely reach 3.0 mm and had not been observed to exceed 3.3 mm. However, many of the eggs that were genetically identified as originating from native cyprinids were as large as 4.0 mm in diameter (at early developmental stages) and were therefore large enough to over- lap with the lower end of the size range observed for Asian carp eggs. Researchers studying the egg stages of Asian carp and other cyprinids should plan on preserving subsets of eggs for genetic analysis to confirm morphological identifications.

The M-T Hook Structure Is Critical for Design of HIV-1 Fusion Inhibitors*

PubMed Central

Chong, Huihui; Yao, Xue; Sun, Jianping; Qiu, Zonglin; Zhang, Meng; Waltersperger, Sandro; Wang, Meitian; Cui, Sheng; He, Yuxian

2012-01-01

CP621-652 is a potent HIV-1 fusion inhibitor peptide derived from the C-terminal heptad repeat of gp41. We recently identified that its N-terminal residues Met-626 and Thr-627 adopt a unique hook-like structure (termed M-T hook) thus stabilizing the interaction of the inhibitor with the deep pocket on the N-terminal heptad repeat. In this study, we further demonstrated that the M-T hook structure is a key determinant of CP621-652 in terms of its thermostability and anti-HIV activity. To directly define the structure and function of the M-T hook, we generated the peptide MT-C34 by incorporating Met-626 and Thr-627 into the N terminus of the C-terminal heptad repeat-derived peptide C34. The high resolution crystal structure (1.9 Å) of MT-C34 complexed by an N-terminal heptad repeat-derived peptide reveals that the M-T hook conformation is well preserved at the N-terminal extreme of the inhibitor. Strikingly, addition of two hook residues could dramatically enhance the binding affinity and thermostability of 6-helix bundle core. Compared with C34, MT-C34 exhibited significantly increased activity to inhibit HIV-1 envelope-mediated cell fusion (6.6-fold), virus entry (4.5-fold), and replication (6-fold). Mechanistically, MT-C34 had a 10.5-fold higher increase than C34 in blocking 6-helix bundle formation. We further showed that MT-C34 possessed higher potency against T20 (Enfuvirtide, Fuzeon)-resistant HIV-1 variants. Therefore, this study provides convincing data for our proposed concept that the M-T hook structure is critical for designing HIV-1 fusion inhibitors. PMID:22879603

Strategies to control a common carp population by pulsed commercial harvest

USGS Publications Warehouse

Colvin, Michael E.; Pierce, Clay; Stewart, Timothy W.; Grummer, Scott E.

2012-01-01

Commercial fisheries are commonly used to manage nuisance fishes in freshwater systems, but such efforts are often unsuccessful. Strategies for successfully controlling a nuisance population of common carp Cyprinus carpio by pulsed commercial harvest were evaluated with a combination of (1) field sampling, (2) population estimation and CPUE indexing, and (3) simulation using an exponential semidiscrete biomass dynamics model (SDBDM). The range of annual fishing mortalities (F) that resulted in successful control (F = 0.244–0.265) was narrow. Common carp biomass dynamics were sensitive to unintentional underharvest due to high rates of surplus production and a biomass doubling time of 2.7 years. Simulations indicated that biomanipulation never achieved successful control unless supplemental fishing mortality was imposed. Harvest of a majority of annual production was required to achieve successful control, as indicated by the ecotrophic coefficient (EC). Readily available biomass data and tools such as SDBDMs and ECs can be used in an adaptive management framework to successfully control common carp and other nuisance fishes by pulsed commercial fishing.

Application of Rubber Band with Hooks on Both Ends for Vagus Nerve Stimulator Implantation.

PubMed

Hosoyama, Hiroshi; Hanaya, Ryosuke; Otsubo, Toshiaki; Sato, Masanori; Kashida, Yumi; Sugata, Sei; Katagiri, Masaya; Iida, Koji; Arita, Kazunori

2018-03-01

Vagus nerve stimulation (VNS) is a valuable therapeutic option for many types of drug-resistant epilepsy. Muscle hooks and carotid endarterectomy rings have been used for cervical delamination preceding the implantation of stimulation electrodes. The attachment on both sides of a rubber band of Kamiyama-style hanging needles, as are used for scalp and dural retraction during craniotomy, yields a useful tool for VNS implantation. Here we report our experience with this method. We present our method using a rubber band plus hooks and a review of 21 consecutive patients who underwent VNS implantation using our rubber band-plus-hooks method. None of the 21 patients experienced intraoperative or perioperative complications. Hooks placed in connective tissue around the common carotid artery and jugular vein raised the vagus nerve by elevating the carotid sheath. A single surgeon was able to perform all cervical manipulations under a surgical microscope. The average operation time in this series of 21 patients was 137 minutes. The use of hooks attached to both sides of a rubber band rendered VNS implantation safer by lifting the vagus nerve and standardizing the procedure. Copyright © 2017 Elsevier Inc. All rights reserved.

Development of a PCR assay to detect cyprinid herpesvirus 1 in koi and common carp.

PubMed

Viadanna, Pedro H O; Miller-Morgan, Tim; Peterson, Trace; Way, Keith; Stone, David M; Marty, Gary D; Pilarski, Fabiana; Hedrick, Ronald P; Waltzek, Thomas B

2017-02-08

Cyprinid herpesvirus 1 (CyHV1) infects all scaled and color varieties of common carp Cyprinus carpio, including koi. While it is most often associated with unsightly growths known as 'carp pox,' the underlying lesion (epidermal hyperplasia) can arise from a variety of disease processes. CyHV1-induced epidermal hyperplasia may occur transiently in response to water temperature, and thus histopathology cannot be used in isolation to assess CyHV1 infection status. To address this problem, here we describe a PCR assay targeted to the putative thymidine kinase gene of CyHV1. The PCR assay generates a 141 bp amplicon and reliably detects down to 10 copies of control plasmid DNA sequence (analytic sensitivity). The PCR does not cross-detect genomic DNA from cyprinid herpesvirus 2 and 3 (analytic specificity). The CyHV1 PCR effectively detected viral DNA in koi and common carp sampled from various locations in the UK, USA, Brazil, and Japan. Viral DNA was detected in both normal appearing and grossly affected epidermal tissues from koi experiencing natural epizootics. The new CyHV1 PCR provides an additional approach to histopathology for the rapid detection of CyHV1. Analysis of the thymidine kinase gene sequences determined for 7 PCR-positive carp originating from disparate geographical regions identified 3 sequence types, with 1 type occurring in both koi and common carp.

Microsatellite genetic diversity and differentiation of native and introduced grass carp populations in three continents

USGS Publications Warehouse

Chapman, Duane C.; Chen, Qin; Wang, Chenghui; Zhao, Jinlian; Lu, Guoqing; Zsigmond, Jeney; Li, Si-Fa

2012-01-01

Grass carp (Ctenopharyngodon idella), a freshwater species native to China, has been introduced to about 100 countries/regions and poses both biological and environmental challenges to the receiving ecosystems. In this study, we analyzed genetic variation in grass carp from three introduced river systems (Mississippi River Basin in US, Danube River in Hungary, and Tone River in Japan) as well as its native ranges (Yangtze, Pearl, and Amur Rivers) in China using 21 novel microsatellite loci. The allelic richness, observed heterozygosity, and within-population gene diversity were found to be lower in the introduced populations than in the native populations, presumably due to the small founder population size of the former. Significant genetic differentiation was found between all pairwise populations from different rivers. Both principal component analysis and Bayesian clustering analysis revealed obvious genetic distinction between the native and introduced populations. Interestingly, genetic bottlenecks were detected in the Hungarian and Japanese grass carp populations, but not in the North American population, suggesting that the Mississippi River Basin grass carp has experienced rapid population expansion with potential genetic diversification during the half-century since its introduction. Consequently, the combined forces of the founder effect, introduction history, and rapid population expansion help explaining the observed patterns of genetic diversity within and among both native and introduced populations of the grass carp.

Ingestion and ejection of hooks: effects on long-term health and mortality of angler-caught yellowfin bream Acanthopagrus australis.

PubMed

Broadhurst, Matt K; Butcher, Paul A; Brand, Craig P; Porter, Mark

2007-02-08

Ninety juvenile yellowfin bream Acanthopagrus australis were angled from holding tanks, allowed to ingest nickel-plated, carbon-steel J-hooks and released (with their lines cut) into individual experimental tanks during 2 experiments in order to assess their (1) long-term (up to 105 d) health, mortality and rate of hook ejection and (2) short- and medium-term (< 42 d) temporal changes in health during hook ingestion. Equal numbers of control fish were scooped from holding tanks and similarly monitored in experimental tanks. Of 20 hook-ingested fish released during Expt 1, 3 died within 8 d, providing a non-significant mortality of 15%. Between Day 6 and Day 56 post-release, 13 of the surviving individuals ejected their hooks, which were typically oxidized to about 94% of their original weight and often broken into 2 pieces. At Day 105, there were no significant differences between the 20 control and 17 hook-ingested/-ejected fish in terms of their ability to digest and assimilate food (measured as changes in apparent digestibility coefficients), stress (measured as concentrations of plasma cortisol and glucose) or of morphological parameters that included weight (Wt) and maximum height (MH), maximum width (MW) and maximum girth (MG). During Expt 2, 3 individuals that still contained ingested hooks and 3 controls were sampled on each of 9 occasions between Day 3 and Day 42 post-release. All fish were sampled for blood cortisol and glucose and were then euthanized before being weighed and measured for total length (TL), MH, MW and MG. Hook-ingested individuals were also X-rayed to determine the position and orientation of hooks. There were no significant differences in plasma glucose between hook-ingested and control fish. Irrespective of the treatment of fish, concentrations of cortisol were elevated on some sampling occasions, indicating variable, acute stress. The MH and MG of fish were not significantly different between groups. Significant differences were

Using pharyngeal teeth and chewing pads to estimate juvenile Silver Carp total length in the La Grange Reach, Illinois River

USGS Publications Warehouse

Lampo, Eli G.; Knights, Brent C.; Vallazza, Jon; Anderson, Cory A.; Rechkemmer, Will T.; Solomon, Levi E.; Casper, Andrew F.; Pendleton, Richard M.; Lamer, James T.

2017-01-01

The Silver Carp Hypophthalmichthys molitrix is an invasive species in the Mississippi River basin; an understanding of their vulnerability to predation as juveniles may inform control by native predators and predator enhancement (e.g., stocking). Digestion of Silver Carp prey recovered from diets makes it difficult to determine the size‐classes that are most vulnerable to predation by native fishes. The objective of this study was to determine whether the sizes of the chewing pad (CP), pharyngeal teeth (PT), and pharyngeal arch (PA)—the Silver Carp structures most often found intact in predator diets—were predictive of the TL of prey Silver Carp. During 2014 and 2015, juvenile Silver Carp (n = 136; <180 mm) were collected using 60‐Hz pulsed‐DC electrofishing and mini‐fyke nets in the La Grange reach of the Illinois River. We extracted Silver Carp CPs (n = 136 fish) and PAs with PT intact (n = 129 fish) and measured CP length (CPL) and width (CPW), eight reproducible PT landmarks (PT1L–PT4L; PT1W–PT4W), and four reproducible PA landmarks (PA1–PA4) to the nearest 0.01 µm. Using simple linear regression, we found a strong predictive relationship between measurements of CP, PT, or PA and the TL of Silver Carp. The CPL (r2 = 0.94) and CPW (r2 = 0.94) had the strongest relationships with Silver Carp TL, followed by PA1 (r2 = 0.89) and PT1L (r2 = 0.87). These strong relationships suggest that all three structures could be used in diet analyses to accurately estimate Silver Carp TL and thus further our understanding of predator–prey dynamics for this high‐risk invasive species.

The invasive fish tapeworm Atractolytocestus huronensis (Cestoda), a parasite of carp, colonises Africa.

PubMed

Scholz, Tomáš; Tavakol, Sareh; Halajian, Ali; Luus-Powell, Wilmien J

2015-09-01

Biological invasions represent a serious threat for aquaculture because many of introduced parasites may negatively affect the health state of feral and cultured fish. In the present account, the invasive tapeworm Atractolytocestus huronensis Anthony, 1958 (Cestoda: Caryophyllidea), which was originally described from North America and has been introduced to Europe including the British Isles with its specific host, common carp (Cyprinus carpio L.), is reported from Africa for the first time. Its recent introduction to South Africa, where it was found in four localities where common carp is cultured, is another evidence of insufficient prophylactic measures and inadequate veterinary control during transfers of cultured fish, especially common carp, between continents. Together with the Asian fish tapeworm, Bothriocephalus acheilognathi, A. huronensis is another fish tapeworm with ability to spread throughout the globe as a result of man-made introductions of its fish hosts.

Evaluation in vitro of the tensile strength of crimpable hooks used for stabilization in orthognathic surgery.

PubMed

Andrade Gomes, Nascimento Leonard Euller; Melo, Pithon Matheus; Lacerda, Santos Rogério; D'Albuquerque, Medeiros Paulo Jose

2012-03-01

The aim of this study was to evaluate the tensile strength of crimpable hooks used for arch stabilization in orthognatic surgery. Ninety stainless steel wire (0.019" × 0.026") segments, each measuring 6 cm long, were used and attached to crimpable hooks of different commercial brands. Six groups were formed (n = 10) denominated as follows: control, in which the wire segments were perpendicularly welded by spot welding machine; and the hooks groups M (Morelli), MS (Morelli with weld), TP (TP Ortho), TPS (TP with weld), TPTg (TP-tungsten), TPTgS (TP-tungsten with weld), AO (American Orthodontics), and AOS (American Orthodontics with weld). The test specimen topography was evaluated by scanning electron microscopy before and after the tensile strength tests. After obtaining the results, the analysis of variance and Kruskal-Wallis multiple-comparison tests were applied. Compared with the control group, the AOS ball hooks and those from TPS required a greater amount of force to be displaced along the rectangular arch. The hooks in group M were significantly easier to displace, followed by those from AO. The best ball hooks for clinical application are those from AOS and TPS. Copyright © 2012 Elsevier Inc. All rights reserved.

Pesticide-contaminated feeds in integrated grass carp aquaculture: toxicology and bioaccumulation.

PubMed

Pucher, J; Gut, T; Mayrhofer, R; El-Matbouli, M; Viet, P H; Ngoc, N T; Lamers, M; Streck, T; Focken, U

2014-02-19

Effects of dissolved pesticides on fish are widely described, but little is known about effects of pesticide-contaminated feeds taken up orally by fish. In integrated farms, pesticides used on crops may affect grass carp that feed on plants from these fields. In northern Vietnam, grass carp suffer seasonal mass mortalities which may be caused by pesticide-contaminated plants. To test effects of pesticide-contaminated feeds on health and bioaccumulation in grass carp, a net-cage trial was conducted with 5 differently contaminated grasses. Grass was spiked with 2 levels of trichlorfon/fenitrothion and fenobucarb. Unspiked grass was used as a control. Fish were fed at a daily rate of 20% of body mass for 10 d. The concentrations of fenitrothion and fenobucarb in pond water increased over time. Effects on fish mortality were not found. Fenobucarb in feed showed the strongest effects on fish by lowering feed uptake, deforming the liver, increasing blood glucose and reducing cholinesterase activity in blood serum, depending on feed uptake. Fenobucarb showed increased levels in flesh in all treatments, suggesting bio-concentration. Trichlorfon and fenitrothion did not significantly affect feed uptake but showed concentration-dependent reduction of cholinesterase activity and liver changes. Fenitrothion showed bioaccumulation in flesh which was dependant on feed uptake, whereas trichlorfon was only detected in very low concentrations in all treatments. Pesticide levels were all detected below the maximum residue levels in food. The pesticide-contaminated feeds tested did not cause mortality in grass carp but were associated with negative physiological responses and may increase susceptibility to diseases.

Prevalence of Ingested Fish Hooks in Freshwater Turtles from Five Rivers in the Southeastern United States

PubMed Central

Steen, David A.; Hopkins, Brittney C.; Van Dyke, James U.; Hopkins, William A.

2014-01-01

Freshwater turtles may ingest baited fish hooks because many are opportunistic scavengers. Although the ingestion of fish hooks is known to be a source of mortality in multiple vertebrate groups, the prevalence of hook ingestion by freshwater turtles has not been well studied. We trapped turtles from five rivers in the southeastern United States and used radiographs to examine over 600 individuals of four species. Depending on the species, sex, and age class, 0–33% of turtles contained ingested fish hooks. For some species, larger turtles were more likely to contain a fish hook than smaller individuals. Freshwater turtle demography suggests that even small increases in adult mortality may lead to population declines. If our study areas are representative of other aquatic systems that receive fishing pressure, this work likely identifies a potential conflict between a widespread, common recreational activity (i.e., fishing) and an imperiled taxonomic group. PMID:24621919

Designing for sustainability: ergonomics--carpe diem.

PubMed

Martin, K; Legg, S; Brown, C

2013-01-01

Sustainability is a global issue that has worldwide attention but the role of ergonomics in designing for sustainability is poorly understood and seldom considered. An analysis of the literature on ergonomics, design and sustainability was conducted via a search of electronic databases: Scopus, Business Source Complete, Google Scholar, Emerald Publishing, Academic Search Premiere, Web of Science, Discover and Ergonomics Abstracts, for the years 1995-2012. A total of 1934 articles fulfilled the search criteria, but content analysis of the abstracts indicated that only 14 refereed articles addressed the main search criteria. Of those seven were in ergonomics journals and seven were in other journals (and were not written by ergonomists). It is concluded that the contribution of ergonomics to sustainability and sustainable design has been limited, even though the goals of sustainability and ergonomics are congruent. Ergonomists have not been at the forefront of research contributing to sustainability - and it is time for them to 'seize the day' - 'carpe diem'. This literature review shows that ergonomics contribution to sustainability is limited but since there is congruence between the disciplines it calls for ergonomists to become more involved and to seize the day - carpe diem.

Metoprolol induces oxidative damage in common carp (Cyprinus carpio).

PubMed

Martínez-Rodríguez, Héctor; Donkor, Kingsley; Brewer, Sharon; Galar-Martínez, Marcela; SanJuan-Reyes, Nely; Islas-Flores, Hariz; Sánchez-Aceves, Livier; Elizalde-Velázquez, Armando; Gómez-Oliván, Leobardo Manuel

2018-04-01

During the last decade, β-blockers such as metoprolol (MTP) have been frequently detected in surface water, aquatic systems and municipal water at concentrations of ng/L to μg/L. Only a small number of studies exist on the toxic effects induced by this group of pharmaceuticals on aquatic organisms. Therefore, the present study aimed to evaluate the oxidative damage induced by MTP in the common carp Cyprinus carpio, using oxidative stress biomarkers. To this end, indicators of cellular oxidation such as hydroperoxide content (HPC), lipid peroxidation (LPX) and protein carbonyl content (PCC) were determined, as well as the activity of the antioxidant enzymes superoxide dismutase (SOD) and catalase (CAT). Also, concentrations of MTP and its metabolite O-desmethyl metoprolol were determined in water as well as carp gill, liver, kidney, brain and blood, along with the partial uptake pattern of these compounds. Results show that carp takes up MTP and its metabolite in the different organs evaluated, particularly liver and gill. The oxidative stress biomarkers, HPC, LPX, and PCC, as well as SOD and CAT activity all increased significantly at most exposure times in all organs evaluated. Results indicate that MTP and its metabolite induce oxidative stress on the teleost C. carpio and that the presence of these compounds may constitute a risk in water bodies for aquatic species. Copyright © 2018 Elsevier B.V. All rights reserved.

Kinematics, hydrodynamics and energetic advantages of burst-and-coast swimming of koi carps (Cyprinus carpio koi).

PubMed

Wu, Guanhao; Yang, Yan; Zeng, Lijiang

2007-06-01

Koi carps frequently swim in burst-and-coast style, which consists of a burst phase and a coast phase. We quantify the swimming kinematics and the flow patterns generated by the carps in burst-and-coast swimming. In the burst phase, the carps burst in two modes: in the first, the tail beats for at least one cycle (multiple tail-beat mode); in the second, the tail beats for only a half-cycle (half tail-beat mode). The carp generates a vortex ring in each half-cycle beat. The vortex rings generated during bursting in multiple tail-beat mode form a linked chain, but only one vortex ring is generated in half tail-beat mode. The wake morphologies, such as momentum angle and jet angle, also show much difference between the two modes. In the burst phase, the kinematic data and the impulse obtained from the wake are linked to obtain the drag coefficient (C(d,burst) approximately 0.242). In the coast phase, drag coefficient (C(d,coast) approximately 0.060) is estimated from swimming speed deceleration. Our estimation suggests that nearly 45% of energy is saved when burst-and-coast swimming is used by the koi carps compared with steady swimming at the same mean speed.

Casual hook up sex during the first year of college: Prospective associations with attitudes about sex and love relationships.

PubMed

Katz, Jennifer; Schneider, Monica E

2013-11-01

This study examined bidirectional relationships among emerging adults' involvement in casual hook up sex and attitudes about sex and love relationships. At the start and end of their first year in college, undergraduates (N = 163) responded to measures of sexual behavior, sexual attitudes, and attitudes about love relationships. In cross-sectional analyses, attitudes about sex and love both were associated with involvement in casual hook up sex. In prospective analyses, initial attitudes about sexual instrumentality uniquely predicted involvement in later hook up sex, even after controlling for past hook up sex. Furthermore, involvement in hook up sex during the first year of college predicted greater sexual permissiveness and comfort with casual genital contact, even after controlling for initial sexual attitudes and hook up behaviors. None of the associations between attitudes and behavior were qualified by gender. Experiences of causal hook up sex appear to have implications primarily for emerging adults' attitudes about sexual interactions rather than their attitudes about love relationships.

The clavicle hook plate for Neer type II lateral clavicle fractures.

PubMed

Renger, R J; Roukema, G R; Reurings, J C; Raams, P M; Font, J; Verleisdonk, E J M M

2009-09-01

To evaluate functional and radiologic outcome in patients with a Neer type II lateral clavicle fracture treated with the clavicle hook plate. Multicenter retrospective study. Five level I and II trauma centers. Forty-four patients, average age 38.4 years (18-66 years), with a Neer type II lateral clavicle fracture treated with the clavicle hook plate between January 1, 2003, and December 31, 2006. Open reduction and internal fixation with the clavicle hook plate. Removal of all 44 implants after consolidation at a mean of 8.4 months (2-33 months) postoperatively. At an average follow-up of 27.4 months (13-48 months), functional outcome was assessed with the Constant-Murley scoring system. Radiographs were taken to evaluate consolidation and to determine the distance between the coracoid process and the clavicle. The average Constant score was 92.4 (74-100). The average distance between the coracoid process and the clavicle was 9.8 mm (7.3-14.8 mm) compared with 9.4 mm (6.9-14.3 mm) on the contralateral nonoperative side. We observed 1 dislocation of an implant (2.2%), 2 cases of pseudarthrosis (4.5%), 2 superficial wound infections (4.5%), 2 patients with hypertrophic scar tissue (4.5%), and 3 times an acromial osteolysis (6.8%). Thirty patients (68%) reported discomfort due to the implant. These implant-related complaints and the acromial osteolysis disappeared after removal of the hook plate. With all the patients, direct functional aftercare was possible. The clavicle hook plate is a suitable implant for Neer type II clavicle fractures. The advantage of this osteosynthesis is the possibility of immediate functional aftercare. We observed a high percentage of discomfort due to the implant; therefore, we advise to remove the implant as soon as consolidation has taken place.

Cloning and preliminary functional studies of the JAM-A gene in grass carp (Ctenopharyngodon idellus).

PubMed

Du, Fukuan; Su, Jianguo; Huang, Rong; Liao, Lanjie; Zhu, Zuoyan; Wang, Yaping

2013-06-01

Grass carp (Ctenopharyngodon idellus) is a very important aquaculture species in China and other South-East Asian countries; however, disease outbreaks in this species are frequent, resulting in huge economic losses. Grass carp hemorrhage caused by grass carp reovirus (GCRV) is one of the most serious diseases. Junction adhesion molecule A (JAM-A) is the mammalian receptor for reovirus, and has been well studied. However, the JAM-A gene in grass carp has not been studied so far. In this study, we cloned and elucidated the structure of the JAM-A gene in grass carp (GcJAM-A) and then studied its functions during grass carp hemorrhage. GcJAM-A is composed of 10 exons and 9 introns, and its full-length cDNA is 1833 bp long, with an 888 bp open reading frame (ORF) that encodes a 295 amino acid protein. The GcJAM-A protein is predicted to contain a typical transmembrane domain. Maternal expression pattern of GcJAM-A is observed during early embryogenesis, while zygote expression occurs at 8 h after hatching. GcJAM-A is expressed strongly in the gill, liver, intestine and kidney, while it is expressed poorly in the blood, brain, spleen and head kidney. Moreover, lower expression is observed in the gill, liver, intestine, brain, spleen and kidney of 30-month-old individuals, compared with 6-month-old. In a GcJAM-A-knockdown cell line (CIK) infected with GCRV, the expression of genes involved in the interferon and apoptosis pathways was significantly inhibited. These results suggest that GcJAM-A could be a receptor for GCRV. We have therefore managed to characterize the GcJAM-A gene and provide evidence for its role as a receptor for GCRV. Crown Copyright © 2013. Published by Elsevier Ltd. All rights reserved.

Floodplain Wetlands as Nurseries for Silver Carp, Hypophthalmichthys Molitrix: A Conceptual Model for Use in Managing Local Populations

DTIC Science & Technology

2007-11-01

07-4 November 2007 a. Silver carp b. Long, winding intestines of a silver carp filled with phytoplankton c. Egg mass of a mature female silver carp...10 μm) phytoplankton and large mucilaginous algae (Voros et al. 1997, Domaizon and Devaux 1999, Radke and Kahl 2002). Impacts in small (ə km2...zooplankton and large phytoplankton will reduce food supplies for planktivorous fishes which may explain the low numbers of shad present (Table 2) and

Fish hook injury: removal by ''push through and cut off'' technique: a case report and brief literature review.

PubMed

Ahmad Khan, Hayat; Kamal, Younis; Lone, Ansar Ul Haq

2014-04-01

Fishing is a leisure activity for some people around the world. Accidently the fish hook can get hooked in the hand. If the hook is barbed, removal becomes difficult. We report a case of such a injury in the hand and discuss the technique for its removal with a brief review of the literature. A thirty-two year old male accidently suffered a fishhook injury to his hand. He came to the orthopaedic ward two hours after the incident with pain; the fish hook was hanging from the hand. Unsuccessful attempts to remove it were made by his relatives. A push-through and cut-off technique was used for removal of barbed hook. Barbed hooks are to be removed atraumatically with controlled incision over properly anaesthetised skin. Proper wound management and prophylactic antibiotics suitable for treatment of Aeromonas species should be initiated to prevent complications.

Embryonic and larval development and early behavior in grass carp, Ctenopharyngodon idella: implications for recruitment in rivers

USGS Publications Warehouse

George, Amy E.; Chapman, Duane C.

2015-01-01

With recent findings of grass carp Ctenopharyngodon idella in tributaries of the Great Lakes, information on developmental rate and larval behavior is critical to efforts to assess the potential for establishment within the tributaries of that region. In laboratory experiments, grass carp were spawned and eggs and larvae reared at two temperature treatments, one "cold" and one "warm", and tracked for developmental rate, egg size, and behavior. Developmental rate was quantified using Yi's (1988) developmental stages and the cumulative thermal units method. Grass carp had a thermal minimum of 13.5°C for embryonic stages and 13.3°C for larval stages. Egg size was related to temperature and maternal size, with the largest eggs coming from the largest females, and eggs were generally larger in warmer treatments. Young grass carp larvae exhibited upward and downward swimming interspersed with long periods of lying on the bottom. Swimming capacity increased with ontogeny, and larvae were capable of horizontal swimming and position holding with gas bladder emergence. Developmental rates, behavior, and egg attributes can be used in combination with physical parameters of a river to assess the risk that grass carp are capable of reproduction and recruitment in rivers.

Effect of mercury chloride to number of melano-macrophage centers on the kidney of carp fish (Cyprinus carpio)

NASA Astrophysics Data System (ADS)

Mubarokah, L.; Tjahjaningsih, W.; Sulmartiwi, L.

2018-04-01

Mercury chloride can cause immunotoxic effects on fish. The accumulation or aggregate of melano-macrophages centers (MMCs) in the kidney is a feature of cellular immune response, so it can be used as a bioindicator of heavy metal toxicity in waters. This research aims to determine an effect of heavy metal exposure concentration of mercury chloride (HgCl2) on a number of MMCs from common carp kidney. This research using four treatments of mercury chloride: 0 ppm, 0.01 ppm, 0.05 ppm and 0.1 ppm. The main parameters were the number of MMCs in kidney common carp. Supporting parameter was behavior change, water quality and concentration of mercury in the water and kidney. The results of the research showed that the concentration the heavy metal exposure concentration of mercury chloride (HgCl2) affected the number of MMCs from common carp (Cyprinus carpio) kidney. The number of MMCs in the kidney of common carp exposed to mercury chloride 0.01 ppm, 0.05 ppm and 0.1 ppm increased compared to carp was not exposed to mercury chloride (0 ppm).

Passing of northern pike and common carp through experimental barriers designed for use in wetland restoration

USGS Publications Warehouse

French, John R. P.; Wilcox, Douglas A.; Nichols, S. Jerrine

1999-01-01

Restoration plans for Metzger Marsh, a coastal wetland on the south shore of western Lake Erie, incorporated a fish-control system designed to restrict access to the wetland by large common carp (Cyprinus carpio). Ingress fish passageways in the structure contain slots into which experimental grates of varying size and shape can be placed to selectively allow entry and transfer of other large fish species while minimizing the number of common carp to be handled. We tested different sizes and shapes of grates in experimental tanks in the laboratory to determine the best design for testing in the field. We also tested northern pike (Esox lucius) because lack of access to wetland spawning habitat has greatly reduced their populations in western Lake Erie. Based on our results, vertical bar grates were chosen for installation because common carp were able to pass through circular grates smaller than body height by compressing their soft abdomens; they passed through rectangular grates on the diagonal. Vertical bar grates with 5-cm spacing that were installed across much of the control structure should limit access of common carp larger than 34 cm total length (TL) and northern pike larger than 70 cm. Vertical bar grates selected for initial field trials in the fish passageway had spacings of 5.8 and 6.6 cm, which increased access by common carp to 40 and 47 cm TL and by northern pike to 76 and 81 cm, respectively. The percentage of potential common carp biomass (fish seeking entry) that must be handled in lift baskets in the passageway increased from 0.9 to 4.8 to 15.4 with each increase in spacing between bars. Further increases in spacing would greatly increase the number of common carp that would have to be handled. The results of field testing should be useful in designing selective fish-control systems for other wetland restoration sites adjacent to large water bodies.

De novo assembly and characterization of the spleen transcriptome of common carp (Cyprinus carpio) using Illumina paired-end sequencing.

PubMed

Li, Guoxi; Zhao, Yinli; Liu, Zhonghu; Gao, Chunsheng; Yan, Fengbin; Liu, Bianzhi; Feng, Jianxin

2015-06-01

Common carp (Cyprinus carpio) is one of the most important aquacultured species of the family Cyprinidae, and breeding this species for disease resistance is becoming more and more important. However, at the genome or transcriptome levels, study of the immunogenetics of disease resistance in the common carp is lacking. In this study, 60,316,906 and 75,200,328 paired-end clean reads were obtained from two cDNA libraries of the common carp spleen by Illumina paired-end sequencing technology. Totally, 130,293 unique transcript fragments (unigenes) were assembled, with an average length of 1400.57 bp. Approximately 105,612 (81.06%) unigenes could be annotated according to their homology with matches in the Nr, Nt, Swiss-Prot, COG, GO, or KEGG databases, and they were found to represent 46,747 non-redundant genes. Comparative analysis showed that 59.82% of the unigenes have significant similarity to zebrafish Refseq proteins. Gene expression comparison revealed that 10,432 and 6889 annotated unigenes were, respectively, up- and down-regulated with at least twofold changes between two developmental stages of the common carp spleen. Gene ontology and KEGG analysis were performed to classify all unigenes into functional categories for understanding gene functions and regulation pathways. In addition, 46,847 simple sequence repeats (SSRs) were detected from 35,618 unigenes, and a large number of single nucleotide polymorphism (SNP) and insertion/deletion (INDEL) sites were identified in the spleen transcriptome of common carp. This study has characterized the spleen transcriptome of the common carp for the first time, providing a valuable resource for a better understanding of the common carp immune system and defense mechanisms. This knowledge will also facilitate future functional studies on common carp immunogenetics that may eventually be applied in breeding programs. Copyright © 2015 Elsevier Ltd. All rights reserved.

Abnormally low hCG in a complete hydatidiform molar pregnancy: The hook effect.

PubMed

Nodler, James L; Kim, Kenneth H; Alvarez, Ronald D

2011-01-01

► The hook effect occurs with extremely high levels of hCG, saturating detection antibodies, leading to falsely low laboratory results. ► In the literature, descriptions of the hook effect are rare in cases of gestational trophoblastic diseases. ► If unrecognized, this can lead to delayed therapy or mismanagement of care.

Morphological and histochemical demonstration of hooks in the rostellar region of Cysticercus bovis.

PubMed

Zdárská, Z

1976-01-01

Hooklets and early developmental stages of hooks were discovered in the tegument among the microtriches in the rostellar region of 83- and 108-day-old Cysticercus bovis. The cortical layer of hooks consists of proteins with tyrosine and SS groups. The rostellar sac and bulb are distinctly developed in the parenchyma of the rostellar region.

Altered gut microbiota associated with intestinal disease in grass carp (Ctenopharyngodon idellus).

PubMed

Tran, Ngoc Tuan; Zhang, Jing; Xiong, Fan; Wang, Gui-Tang; Li, Wen-Xiang; Wu, Shan-Gong

2018-05-18

Gut microbiota plays a crucial importance in their host. Disturbance of the microbial structure and function is known to be associated with inflammatory intestinal disorders. Enteritis is a significant cause of high mortality in fish species, including grass carp (Ctenopharyngodon idellus). Study regarding the association between microbial alternations and enteritis in grass carp is still absent. In this study, changes in the gut microbiota of grass carp suffering from enteritis were investigated using NGS-based 16S rRNA sequencing. Six healthy and ten abnormal fish (showing reddening anus, red odiferous fluid accumulating in the abdominal capacity, and flatulence and haemorrhage in the intestine) were collected from a fish farm in Huanggang Fisheries Institute (Hubei, China). Our results revealed that the diversity, structure, and function of gut microbiota were significantly different between diseased and healthy fish (P < 0.05). Particularly, members of the genera Dechloromonas, Methylocaldum, Planctomyces, Rhodobacter, Caulobacter, Flavobacterium, and Pseudomonas were significantly increased in diseased fish compared with that in healthy fish (P < 0.05). Predicted function indicated that microbiota significantly changed the specific metabolic pathways (related to amino acid metabolism, xenobiotics biodegradation and metabolism, and carbohydrate metabolism) in diseased fish (P < 0.05). Taken together, our findings point out the association between changes of the gut microbiota and enteritis in grass carp, which provide basic information useful for diagnoses, prevention, and treatment of intestinal diseases occurring in cultured fish.

Identification and expression analysis of genes involved in early ovary development in diploid gynogenetic hybrids of red crucian carp x common carp.

PubMed

Liu, Dong; Liu, Shaojun; You, Cuiping; Chen, Lin; Liu, Zhen; Liu, Liangguo; Wang, Jing; Liu, Yun

2010-04-01

Diploid eggs of allotetraploid hybrids (red crucian carp female symbol x common carp male symbol), when activated by UV-irradiated sperm of scatter scale carp, can develop into diploid progenies without chromosome duplication treatment. Diploid progenies produce diploid eggs, which develop into diploid population by the same way. To understand the molecular mechanism underlying the production of diploid eggs by the diploid fish, we constructed a forward suppression subtractive hybridization complementary DNA (cDNA) library. The cDNAs from the ovary in proliferation phase were employed as the "tester," and those in growth phase were used as the "driver." Seventy-three cDNA clones that are specifically expressed in proliferation phase were detected by dot-blot hybridization. Sequencing analyses revealed that several of these cDNAs have high homologies to the known sequences in the NCBI database. Their encoded proteins include the protein preventing mitosis catastrophe (PMC), the signal recognition particle 9, the ATP-binding cassette transporter, the glucanase-xylanase fusion protein, and others. These genes were confirmed by reverse transcriptase-polymerase chain reaction. The expression profile of the PMC gene at different time points was analyzed by quantitative real-time polymerase chain reaction. The results indicated that the expression of this suppression subtractive hybridization-identified gene changed during the time course, corresponding with the cellular phenomenon in the ovary development. Our studies provide insights into the molecular mechanism underlying the ovary development of diploid gynogenetic fish.

[Treatment of sternoclavicular joint dislocation with sternoclavicular hook plate fixation].

PubMed

Liu, Pan; Yuan, Jia-bin; Liu, Zhong-qian; Lu, Bing; Wang, Yue

2015-08-01

To evaluate the technique and therapeutic effect of sternoclavicular hook plate fixation in treating sternoclavicular joint (SCJ) dislocation. From January 2010 to March 2014,6 patients with SCJ dislocation were treated with sternoclavicular hook plate fixation in our hospital. Among the 6 patients, 5 patients were male and 1 patient was female, and the average age was 34 years, ranging from 26 to 48 years. The course of the disease ranged from 3 to 20 days. All the SCJ dislocations were caused by external injury and accompanied with the symptoms of swelling pain and obvious shoulder joint activity restricted in affected side. All SCJ dislocations were anterior dislocation by the diagnosis of X-ray and CT scan. The postoperative curative effect was evaluated according to Rockwood score. All the patients' operative incision were healed well and in good appearance. X-ray showed that the dislocated SCJ was well reduced and the plate was on right position. All the 6 patients were followed up for 4 to 18 months, with an average of 12 months. The results were evaluated according to Rockwood score, 4 got excellent results, 1 good and 1 fair. No fixation loosening, redislocation or side injury such as vessel, nerve or pleura injury were found. With sternoclavicular hook plate fixation, SCJ dislocation could be reduced while keeping its amphiarthrodial function and the completeness of the cartilage surface. Sternoclavicular hook plate fixation has advantages of safety and stabilization in fixation, and patients can begin function exercises earlier.

HEALTH SURVEY INCLUDING SELECTED BLOOD PARAMETERS IN THE AFRICAN SLENDER SNOUTED CROCODILE (MECISTOPS CATAPHRACTUS) AT THE ABIDJAN ZOO IN CÔTE D'IVOIRE.

PubMed

Bradford, Carol; Eschenbrenner, Matt

2017-06-01

The Zoo National d'Abidjan (Abidjan Zoo) in Côte d'Ivoire, West Africa, holds the world's largest captive population of African slender-snouted crocodiles (Mecistops cataphractus, formerly Crocodylus cataphractus), at 36 adults, 16 yearlings, and 23 hatchlings. Twelve yearling and 12 adult slender-snouted crocodiles at the Abidjan Zoo were restrained for physical exam, body condition scoring, and venipuncture in September 2015. Blood samples collected from the supravertebral venous sinus were analyzed using a handheld blood analyzer (Abaxis® I-stat, Abaxis, Inc., Union City, California 94587, USA) with Chem8+ cartridges (CLIAwaived, Inc., San Diego, California 92130, USA). The adult crocodiles appeared in good general health and demonstrated blood values similar to those of other reptiles. The yearlings had low, ionized calcium values and low hematocrit and hemoglobin levels compared with the adult crocodiles and to other crocodile reference ranges. These findings may dramatically improve the health of the crocodiles and help to ensure a thriving captive population of this critically endangered species.

Profilings of MicroRNAs in the Liver of Common Carp (Cyprinus carpio) Infected with Flavobacterium columnare

PubMed Central

Zhao, Lijuan; Lu, Hong; Meng, Qinglei; Wang, Jinfu; Wang, Weimin; Yang, Ling; Lin, Li

2016-01-01

MicroRNAs (miRNAs) play important roles in regulation of many biological processes in eukaryotes, including pathogen infection and host interactions. Flavobacterium columnare (FC) infection can cause great economic loss of common carp (Cyprinus carpio) which is one of the most important cultured fish in the world. However, miRNAs in response to FC infection in common carp has not been characterized. To identify specific miRNAs involved in common carp infected with FC, we performed microRNA sequencing using livers of common carp infected with and without FC. A total of 698 miRNAs were identified, including 142 which were identified and deposited in the miRbase database (Available online: http://www.mirbase.org/) and 556 had only predicted miRNAs. Among the deposited miRNAs, eight miRNAs were first identified in common carp. Thirty of the 698 miRNAs were differentially expressed miRNAs (DIE-miRNAs) between the FC infected and control samples. From the DIE-miRNAs, seven were selected randomly and their expression profiles were confirmed to be consistent with the microRNA sequencing results using RT-PCR and qRT-PCR. In addition, a total of 27,363 target genes of the 30 DIE-miRNAs were predicted. The target genes were enriched in five Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways, including focal adhesion, extracellular matrix (ECM)-receptor interaction, erythroblastic leukemia viral oncogene homolog (ErbB) signaling pathway, regulation of actin cytoskeleton, and adherent junction. The miRNA expression profile of the liver of common carp infected with FC will pave the way for the development of effective strategies to fight against FC infection. PMID:27092486

On the reliability of hook echoes as tornado indicators

NASA Technical Reports Server (NTRS)

Forbes, G. S.

1981-01-01

A study of radar echoes associated with the tornadoes of the 3 April 1974 outbreak was performed to evaluate the usefulness of echo shape as an indicator of tornadic thunderstorms. The hook shape was usually successful in characterizing an echo as tornadic, with a false alarm rate of 16%. Because hook echoes were relatively rare, however, a less restrictive shape called distinctive was more successful at detecting tornadic thunderstorms, identifying 65% of the tornadic echoes. An echo had a distinctive shape if it possessed a marked appendage on its right rear flank or was in the shape of a spiral, comma or line echo wave pattern (LEWP). Characteristics of the distinctive echo are given.

Genome-Wide Identification, Characterization and Phylogenetic Analysis of ATP-Binding Cassette (ABC) Transporter Genes in Common Carp (Cyprinus carpio).

PubMed

Liu, Xiang; Li, Shangqi; Peng, Wenzhu; Feng, Shuaisheng; Feng, Jianxin; Mahboob, Shahid; Al-Ghanim, Khalid A; Xu, Peng

2016-01-01

The ATP-binding cassette (ABC) gene family is considered to be one of the largest gene families in all forms of prokaryotic and eukaryotic life. Although the ABC transporter genes have been annotated in some species, detailed information about the ABC superfamily and the evolutionary characterization of ABC genes in common carp (Cyprinus carpio) are still unclear. In this research, we identified 61 ABC transporter genes in the common carp genome. Phylogenetic analysis revealed that they could be classified into seven subfamilies, namely 11 ABCAs, six ABCBs, 19 ABCCs, eight ABCDs, two ABCEs, four ABCFs, and 11 ABCGs. Comparative analysis of the ABC genes in seven vertebrate species including common carp, showed that at least 10 common carp genes were retained from the third round of whole genome duplication, while 12 duplicated ABC genes may have come from the fourth round of whole genome duplication. Gene losses were also observed for 14 ABC genes. Expression profiles of the 61 ABC genes in six common carp tissues (brain, heart, spleen, kidney, intestine, and gill) revealed extensive functional divergence among the ABC genes. Different copies of some genes had tissue-specific expression patterns, which may indicate some gene function specialization. This study provides essential genomic resources for future studies in common carp.

Genome-Wide Identification, Characterization and Phylogenetic Analysis of ATP-Binding Cassette (ABC) Transporter Genes in Common Carp (Cyprinus carpio)

PubMed Central

Peng, Wenzhu; Feng, Shuaisheng; Feng, Jianxin; Mahboob, Shahid; Al-Ghanim, Khalid A.

2016-01-01

The ATP-binding cassette (ABC) gene family is considered to be one of the largest gene families in all forms of prokaryotic and eukaryotic life. Although the ABC transporter genes have been annotated in some species, detailed information about the ABC superfamily and the evolutionary characterization of ABC genes in common carp (Cyprinus carpio) are still unclear. In this research, we identified 61 ABC transporter genes in the common carp genome. Phylogenetic analysis revealed that they could be classified into seven subfamilies, namely 11 ABCAs, six ABCBs, 19 ABCCs, eight ABCDs, two ABCEs, four ABCFs, and 11 ABCGs. Comparative analysis of the ABC genes in seven vertebrate species including common carp, showed that at least 10 common carp genes were retained from the third round of whole genome duplication, while 12 duplicated ABC genes may have come from the fourth round of whole genome duplication. Gene losses were also observed for 14 ABC genes. Expression profiles of the 61 ABC genes in six common carp tissues (brain, heart, spleen, kidney, intestine, and gill) revealed extensive functional divergence among the ABC genes. Different copies of some genes had tissue-specific expression patterns, which may indicate some gene function specialization. This study provides essential genomic resources for future studies in common carp. PMID:27058731

Grayia spinosa (Hook.) Moq.: spiny hopsage

Treesearch

Nancy L. Shaw; Marshall R. Haferkamp; Emerenciana G. Hurd

2008-01-01

The genus Grayia Hook. & Arn., named for the American botanist Asa Gray, contains a single species - spiny hopsage (table 1). Plants are erect to rounded, summer-deciduous shrubs 0.3 to 1.2 (1.5) m tall. Branches are divergent and thorn-tipped, with whitish gray to brownish bark that exfoliates in long strips. Leaves are gray-green, alternate, entire, and fleshy,...

Ultrastructure and chemical composition of the proboscis hooks of Acanthocephalus lucii (Müller, 1776) (Acanthocephala: Palaeacanthocephala) using X-ray elemental analysis.

PubMed

Brázová, Tímea; Poddubnaya, Larisa G; Miss, Noemí Ramírez; Hanzelová, Vladimíra

2014-12-01

The ultrastructure and chemical composition of the proboscis hooks and surrounding tegument of Acanthocephalus lucii (Müller, 1776), a parasite of European perch, Perca fluviatilis Linnaeus, were examined using scanning (SEM) and transmission (TEM) electron microscopy and X-ray microanalysis (EDXA). The blade of middle hooks consists of three layers: an outer homogeneous layer, an inner heterogeneous layer and a central core. TEM observation revealed the presence of hollow tubes, which spaced the central core; fibrous inner hook layer surrounded by an electron-dense margin and the basal tegumental layer filled with electron-dense bodies and outer layer. We found for the first time that the so-called 'epidermal covering' surrounding of the exposed hook blade (outer hook layer) is a modified striped portion of the tegumental layer and there are no special contact sites between these two morphologically different structures, i.e. striped layer of the syncytial tegument and following proper outer hook layer, which is a homogeneous, moderately electron-dense layer of -0.3 μm in thickness. The hook root is embedded into subtegumental fibrous layer. X-ray microanalysis of both the surface and internal parts of A. lucii hooks demonstrated the presence of calcium, magnesium, phosphorus and sulphur. The highest concentration of sulphur was recorded at the tip of hooks, whereas the middle part of the hooks was most rich in calcium, phosphorus and magnesium. The proximal part of the hooks contained lower concentrations of sulphur, calcium and phosphorus. In the proboscis tegument, only two elements, calcium and silicon, were found. The differences observed in the chemical composition of the hook 'epidermal covering' and the proboscis tegument support our ultrastructural findings that the hook tegumental covering is a modified structure compared with that of the general proboscis tegument.

Grass carp in the Great Lakes region: establishment potential, expert perceptions, and re-evaluation of experimental evidence of ecological impact

USGS Publications Warehouse

Wittmann, Marion E.; Jerde, Christopher L.; Howeth, Jennifer G.; Maher, Sean P.; Deines, Andrew M.; Jenkins, Jill A.; Whitledge, Gregory W.; Burbank, Sarah B.; Chadderton, William L.; Mahon, Andrew R.; Tyson, Jeffrey T.; Gantz, Crysta A.; Keller, Reuben P.; Drake, John M.; Lodge, David M.

2014-01-01

Intentional introductions of nonindigenous fishes are increasing globally. While benefits of these introductions are easily quantified, assessments to understand the negative impacts to ecosystems are often difficult, incomplete, or absent. Grass carp (Ctenopharyngodon idella) was originally introduced to the United States as a biocontrol agent, and recent observations of wild, diploid individuals in the Great Lakes basin have spurred interest in re-evaluating its ecological risk. Here, we evaluate the ecological impact of grass carp using expert opinion and a suite of the most up-to-date analytical tools and data (ploidy assessment, eDNA surveillance, species distribution models (SDMs), and meta-analysis). The perceived ecological impact of grass carp by fisheries experts was variable, ranging from unknown to very high. Wild-caught triploid and diploid individuals occurred in multiple Great Lakes waterways, and eDNA surveillance suggests that grass carp are abundant in a major tributary of Lake Michigan. SDMs predicted suitable grass carp climate occurs in all Great Lakes. Meta-analysis showed that grass carp introductions impact both water quality and biota. Novel findings based on updated ecological impact assessment tools indicate that iterative risk assessment of introduced fishes may be warranted.

Detection of environmental DNA of Bigheaded Carps in samples collected from selected locations in the St. Croix River and in the Mississippi River

USGS Publications Warehouse

Amberg, Jon J.; McCalla, S. Grace; Miller, Loren; Sorensen, Peter; Gaikowski, Mark P.

2013-01-01

The use of molecular methods, such as the detection of environmental deoxyribonucleic acid (eDNA), have become an increasingly popular tool in surveillance programs that monitor for the presence of invasive species in aquatic systems. One early application of these methods in aquatic systems was surveillance for DNA of Asian carps (specifically bighead carp Hypophthalmichthys nobilis and silver carp H. molitrix) in water samples taken from the Chicago Area Waterway System. The ability to identify DNA of a species in an environmental sample presents a potentially powerful tool because these sensitive analyses can presumably detect the presence of DNA in water even when the species is not abundant or are difficult to catch or monitor with traditional gear. Prior to research presented in this report, an initial eDNA surveillance effort was completed in selected locations in the Upper Mississippi and St. Croix Rivers in 2011 after the capture of a bighead carp in the St. Croix River near Prescott, WI. Data presented in this report were developed to duplicate the 2011 monitoring results from the Upper Mississippi and St. Croix Rivers and to provide critical insight into the technique to inform future work in these locations. We specifically sought to understand the potential confounding effects of other pathways of eDNA movement (e.g., fish-eating birds, watercraft) on the variation in background DNA by collecting water samples from (1) sites within the St. Croix River and the upper Mississippi River where the DNA of silver carp was previously detected, (2) sites considered to be free of Asian carp, and (3) a site known to have a large population of Asian carp. We also sought to establish a baseline Asian carp eDNA signature to which future eDNA sampling efforts could be compared. All samples taken as part of this effort were processed using conventional polymerase chain reaction (PCR) according to procedures outlined in the U.S. Army Corps of Engineers Quality

Organic Chemical Concentrations and Reproductive Biomarkers in Common Carp (Cyprinus carpio) Collected from Two Areas in Lake Mead, Nevada, May 1999-May 2000

USGS Publications Warehouse

Goodbred, Steven L.; Leiker, Thomas J.; Patiño, Reynaldo; Jenkins, Jill A.; Denslow, Nancy D.; Orsak, Erik; Rosen, Michael R.

2007-01-01

The U.S. Geological Survey, in cooperation with the U.S. Fish and Wildlife Service, National Park Service, Bureau of Reclamation, and Nevada Department of Wildlife, collected and assessed data to determine the general health and reproductive status of common carp (Cyprinus carpio) at two study areas in Lake Mead, Nevada, during May 1999-May 2000. These data will form the basis of interpretations and provide a comparison for continuing studies on the health of the ecosystem in Lake Mead. One study area, Las Vegas Bay, is in the western part of Lake Mead. Las Vegas Bay receives inflows from Las Vegas Wash, which is predominantly tertiary-treated wastewater effluent, and to a lesser extent stormwater runoff from Las Vegas, Henderson, and other nearby communities, and from ground water underlying Las Vegas Valley. The other study area, Overton Arm, is in the northern extent of Lake Mead. Overton Arm receives inflow from the Virgin and Muddy Rivers, which historically are not influenced by wastewater effluent. Both sexes of common carp were collected bimonthly for 12 months using boat-mounted electrofishing gear (a direct electric current is used to temporarily immobilize fish for capture) to determine their health and reproductive status and any relation between these factors and environmental contaminants. This report presents fish tissue chemistry, organic chemical compound concentrations, and biomarker data for 83 male common carp collected from Las Vegas Bay, similar organic chemistry results for 15 male common carp, and similar biomarker measures for 80 male common carp collected from Overton Arm. Tissue chemistry results also are presented for 16 female common carp and biomarker measures for 79 female common carp collected from Las Vegas Bay, and tissue chemistry results for 15 female common carp and biomarker measures for 81 female common carp collected from Overton Arm. Thirty-three organic chemical compounds plus total concentrations for four groups of

Comparison of concentric needle versus hooked-wire electrodes in the canine larynx.

PubMed

Jaffe, D M; Solomon, N P; Robinson, R A; Hoffman, H T; Luschei, E S

1998-05-01

The use of a specific electrode type in laryngeal electromyography has not been standardized. Laryngeal electromyography is usually performed with hooked-wire electrodes or concentric needle electrodes. Hooked-wire electrodes have the advantage of allowing laryngeal movement with ease and comfort, whereas the concentric needle electrodes have benefits from a technical aspect and may be advanced, withdrawn, or redirected during attempts to appropriately place the electrode. This study examines whether hooked-wire electrodes permit more stable recordings than standard concentric needle electrodes at rest and after large-scale movements of the larynx and surrounding structures. A histologic comparison of tissue injury resulting from placement and removal of the two electrode types is also made by evaluation of the vocal folds. Electrodes were percutaneously placed into the thyroarytenoid muscles of 10 adult canines. Amplitude of electromyographic activity was measured and compared during vagal stimulation before and after large-scale laryngeal movements. Signal consistency over time was examined. Animals were killed and vocal fold injury was graded and compared histologically. Waveform morphology did not consistently differ between electrode types. The variability of electromyographic amplitude was greater for the hooked-wire electrode (p < 0.05), whereas the mean amplitude measures before and after large-scale laryngeal movements did not differ (p > 0.05). Inflammatory responses and hematoma formation were also similar. Waveform morphology of electromyographic signals registered from both electrode types show similar complex action potentials. There is no difference between the hooked-wire electrode and the concentric needle electrode in terms of electrode stability or vocal fold injury in the thyroarytenoid muscle after large-scale laryngeal movements.

49 CFR 393.134 - What are the rules for securing roll-on/roll-off or hook lift containers?

Code of Federal Regulations, 2011 CFR

2011-10-01

... or hook lift containers? 393.134 Section 393.134 Transportation Other Regulations Relating to... for securing roll-on/roll-off or hook lift containers? (a) Applicability. The rules in this section apply to the transportation of roll-on/roll-off or hook lift containers. (b) Securement of a roll-on...

49 CFR 393.134 - What are the rules for securing roll-on/roll-off or hook lift containers?

Code of Federal Regulations, 2010 CFR

2010-10-01

... or hook lift containers? 393.134 Section 393.134 Transportation Other Regulations Relating to... for securing roll-on/roll-off or hook lift containers? (a) Applicability. The rules in this section apply to the transportation of roll-on/roll-off or hook lift containers. (b) Securement of a roll-on...

Secondary neutron dose measurement for proton eye treatment using an eye snout with a borated neutron absorber

PubMed Central

2013-01-01

Background We measured and assessed ways to reduce the secondary neutron dose from a system for proton eye treatment. Methods Proton beams of 60.30 MeV were delivered through an eye-treatment snout in passive scattering mode. Allyl diglycol carbonate (CR-39) etch detectors were used to measure the neutron dose in the external field at 0.00, 1.64, and 6.00 cm depths in a water phantom. Secondary neutron doses were measured and compared between those with and without a high-hydrogen–boron-containing block. In addition, the neutron energy and vertices distribution were obtained by using a Geant4 Monte Carlo simulation. Results The ratio of the maximum neutron dose equivalent to the proton absorbed dose (H(10)/D) at 2.00 cm from the beam field edge was 8.79 ± 1.28 mSv/Gy. The ratio of the neutron dose equivalent to the proton absorbed dose with and without a high hydrogen-boron containing block was 0.63 ± 0.06 to 1.15 ± 0.13 mSv/Gy at 2.00 cm from the edge of the field at depths of 0.00, 1.64, and 6.00 cm. Conclusions We found that the out-of-field secondary neutron dose in proton eye treatment with an eye snout is relatively small, and it can be further reduced by installing a borated neutron absorbing material. PMID:23866307

Biomechanical Analysis of Implanted Clavicle Hook Plates With Different Implant Depths and Materials in the Acromioclavicular Joint: A Finite Element Analysis Study.

PubMed

Lee, Cheng-Hung; Shih, Cheng-Min; Huang, Kui-Chou; Chen, Kun-Hui; Hung, Li-Kun; Su, Kuo-Chih

2016-11-01

Clinical implantation of clavicle hook plates is often used as a treatment for acromioclavicular joint dislocation. However, it is not uncommon to find patients that have developed acromion osteolysis or had peri-implant fracture after hook plate fixation. With the aim of preventing complications or fixation failure caused by implantation of inappropriate clavicle hook plates, the present study investigated the biomechanics of clavicle hook plates made of different materials and with different hook depths in treating acromioclavicular joint dislocation, using finite element analysis (FEA). This study established four parts using computer models: the clavicle, acromion, clavicle hook plate, and screws, and these established models were used for FEA. Moreover, implantations of clavicle hook plates made of different materials (stainless steel and titanium alloy) and with different depths (12, 15, and 18 mm) in patients with acromioclavicular joint dislocation were simulated in the biomechanical analysis. The results indicate that deeper implantation of the clavicle hook plate reduces stress on the clavicle, and also reduces the force applied to the acromion by the clavicle hook plate. Even though a clavicle hook plate made of titanium alloy (a material with a lower Young's modulus) reduces the force applied to the acromion by the clavicle hook plate, slightly higher stress on the clavicle may occur. The results obtained in this study provide a better reference for orthopedic surgeons in choosing different clavicle hook plates for surgery. Copyright © 2016 International Center for Artificial Organs and Transplantation and Wiley Periodicals, Inc.

Alteration in the cytokine levels and histopathological damage in common carp induced by glyphosate.

PubMed

Ma, Junguo; Li, Xiaoyu

2015-06-01

Glyphosate is one of the most frequently used herbicides, and it has been demonstrated to generate a series of toxicological problems in animals and humans. However, relatively little is known about the effects of glyphosate on the immune system of fish. In the present study, the acute toxicity of glyphosate on common carp was first determined; then, the contents of interferon-γ (IFN-γ), interleukin-1β (IL-1β), and tumor necrosis factor -α (TNF-α) and histopathological alterations in the liver, kidneys, and spleen of common carp exposed to 52.08 or 104.15 mg L(-1) of glyphosate for 168 h were also determined and evaluated. The results of the acute toxicity tests showed that the 96 h LC50 of glyphosate for common carp was 520.77 mg L(-1). Moreover, sub-acute exposure of glyphosate altered the contents of IFN-γ, IL-1β, and TNF-α in fish immune organs. For example, there was a remarkable increase in the IFN-γ content in the kidneys, while there was a decrease in the liver and spleen. The IL-1β content increased in liver and kidneys, but it decreased in the spleen, and TNF-α mainly increased in the fish liver, kidneys, and spleen. In addition, glyphosate-exposure also caused remarkable histopathological damage in the fish liver, kidneys, and spleen. These results suggest that glyphosate-caused cytokine alterations may result in an immune suppression or excessive activation in the treated common carp as well as may cause immune dysfunction or reduced immunity. In conclusion, glyphosate has immunotoxic effects on common carp. Copyright © 2015 Elsevier Ltd. All rights reserved.

Black carp vasa identifies embryonic and gonadal germ cells.

PubMed

Xue, Ting; Yu, Miao; Pan, Qihua; Wang, Yizhou; Fang, Jian; Li, Lingyu; Deng, Yu; Chen, Kai; Wang, Qian; Chen, Tiansheng

2017-07-01

Identification of molecular markers is an essential step in the study of germ cells. Vasa is an RNA helicase and a well-known germ cell marker that plays a crucial role in germ cell development. Here, we identified the Vasa homolog termed Mpvasa as the first germ cell marker in black carp (Mylopharyngodon piceus). First, a 2819-bp full-length Mpvasa complementary DNA (cDNA) was cloned by PCR using degenerated primers of conserved sequences and gene-specific primers. The Mpvasa cDNA sequence encodes a 637-amino acid protein that contains eight conserved characteristic motifs of the DEAD box protein family, and shares high identity to grass carp (81%) and zebrafish (74%) vasa homologs. Second, Mpvasa expression was restricted to the gonad in adulthood by RT-PCR and Western blot analysis. The dynamic patterns of temporal-spatial expression of Mpvasa during gametogenesis were examined by in situ hybridization, and Mpvasa transcripts were strictly detected in gonadal germ cells throughout oogenesis, predominantly in immature oocytes (stage I, II, and III oocytes). Third, Mpvasa transcripts were highly detected in unfertilized eggs and early embryos, and the signal indicated a dynamic migration of the primordial germ cells during embryogenesis, suggesting that Mpvasa transcripts were maternally inherited and specifically distributed in germ cells. Taken together, these results demonstrated that Mpvasa is an applicable molecular marker for identification of gonadal and embryonic germ cells, which facilitates the isolation and utilization of germ cells in black carp.

GIS-based rapid-assessment of bighead carp Hypophthalmichthys nobilis (Richardson, 1845) suitability in reservoirs

USGS Publications Warehouse

Long, James M.; Liang, Yu; Shoup, Daniel E.; Dzialowski, Andrew R.; Bidwell, Joseph R.

2014-01-01

Broad-scale niche models are good for examining the potential for invasive species occurrences, but can fall short in providing managers with site-specific locations for monitoring. Using Oklahoma as an example, where invasive bighead carp (Hypophthalmichthys nobilis) are established in certain reservoirs, but predicted to be widely distributed based on broad-scale niche models, we cast bighead carp reproductive ecology in a site-specific geospatial framework to determine their potential establishment in additional reservoirs. Because bighead carp require large, long free-flowing rivers with suitable hydrology for reproduction but can persist in reservoirs, we considered reservoir tributaries with mean annual daily discharge ≥8.5 cubic meters per second (m3 /s) and quantified the length of their unimpeded portions. In contrast to published broad-scale niche models that identified nearly the entire state as susceptible to invasion, our site-specific models showed that few reservoirs in Oklahoma (N = 9) were suitable for bighead carp establishment. Moreover, this method was rapid and identified sites that could be prioritized for increased study or scrutiny. Our results highlight the importance of considering the environmental characteristics of individual sites, which is often the level at which management efforts are implemented when assessing susceptibility to invasion.

Hot Water Extract of Leather Carp (Cyprinus carpio nudus) Improves Exercise Performance in Mice

PubMed Central

Lee, Gong-Hyeon; Harwanto, Dicky; Park, Sun-Mee; Choi, Jae-Suk; Kim, Mi-Ryung; Hong, Yong-Ki

2015-01-01

The hot water extract of leather carp (Cyprinus carpio nudus) has been used as a nourishing tonic soup and as an aid for recovery from physical fatigue. In this study, we investigated the effect of leather carp extract on exercise performance in mice. Swimming endurance and forelimb grip strength were assessed following oral administration of the extract (once per day for 7 days) at a dose of 0.5 mg/10 μL/g body weight. After 7 days, mice given the leather carp extract had significantly greater swimming endurance [105±18 s (P<0.05); 52% longer than day 0] and forelimb grip strength [1.18±0.05 Newton (P<0.01); 17% greater than day 0]. The extract increased muscle mass, but had little effect on body weight. Following the swimming exercise, blood glucose, glutathione peroxidase, and superoxide dismutase levels in extract-fed mice were significantly higher (145%, 131%, and 106%, respectively) than in the saline control group. Blood levels of high-density lipoprotein cholesterol were also significantly increased (128%) in mice given the extract compared to the controls. These results suggest that leather carp extract can improve physical exercise performance and prevent oxidative stress caused by exhaustive workouts. PMID:26770911

PCBs and other xenobiotics in raw and cooked carp

DOE Office of Scientific and Technical Information (OSTI.GOV)

Zabik, M.E.; Merrill, C.; Zabik, M.J.

The effect of cooking on PCBs and DDT compounds was determined in fillets from carp ranging from 3.0 to 4.9 Kg. Cooking methods included were: poaching, roasting, deep fat frying, charbroiling and cooking by microwave. (JMT)

Physiological response to hooking stress in hatchery and wild rainbow trout (Salmo gairdneri)

USGS Publications Warehouse

Wydoski, R.S.; Wedemeyer, G.A.; Nelson, N. C.

1976-01-01

This study evaluated the physiological response of rainbow trout to hooking stress after being played under standardized conditions (0–5 min) and estimated the time needed for recovery (to 72 h). Plasma osmolality and chloride measurements were used to evaluate osmoregulatory disturbances and gill ion-exchange function, and plasma glucose was used as an index of the generalized nonspecific physiological stress response. Hooking stress caused more severe blood chemistry differences in hatchery fish than in wild trout. Also, hooking stress imposed a greater stress on larger than on smaller hatchery rainbow trout. Higher water temperatures aggravated the delayed hyperglycemia and hyperchloremia in both hatchery and wild trout but only about 3 days were needed for recovery at 4, 10, or 20 C.

Spatial and temporal variation of the gill rakers of gizzard shad and silver carp in three Midwestern rivers

USGS Publications Warehouse

Walleser, Liza R.; Sandheinrich, Mark B.; Howard, David R.; Gaikowski, Mark P.; Amberg, Jon J.

2014-01-01

Improved management of invasive Silver Carp Hypophthalmichthys molitrix in the upper Mississippi River basin may be possible by better understanding the feeding abilities of this population. Food collection for filter-feeding fishes, such as Silver Carp, is influenced by the species-specific structure of their gill rakers. To investigate structural variation in gill rakers of Silver Carp, the morphology of gill rakers was quantified and compared with that of a native filter-feeding fish species which may compete with Silver Carp for food resources, Gizzard Shad Dorosoma cepedianum. Intra- and interspecies variation of gill rakers was examined in both species collected from three locations among four months. Interspecies analysis indicated the size of pores in gill rakers of Silver Carp were much larger than the interraker spacings of Gizzard Shad (95% CI ranged from 80.69 to 185.75 μm versus 16.72 to 47.36 μm, respectively). Intraspecies variation of gill rakers from Silver Carp was related to the overall size of fish and occurred only among sites where dissimilar sizes of fish were collected. This suggested the size of particles filtered by Silver Carp may be dependent upon ontogenic development rather than phenotypic plasticity in response to spatial or temporal factors. Intraspecies variation of gill rakers from Gizzard Shad occurred among site and monthly sampling data; however, variation was only attributable to overall size of fish for monthly sampling data. This suggested ontogeny may influence the filter-feeding ability of this species within a habitat. However, variation noted among sites, which was not attributable to size of fish, may indicate gill rakers are phenotypically plastic among Gizzard Shad populations of various river systems of the upper Mississippi River basin.

The remarkable vision of Robert Hooke (1635-1703): first observer of the microbial world.

PubMed

Gest, Howard

2005-01-01

Robert Hooke played important roles in the early development of the Royal Society of London. As Curator of Experiments of the Society, he became a pioneering microscopist, prolific inventor, astronomer, geologist, architect, and an effective surveyor of the City of London following the Great Fire of 1666. Hooke's Micrographia (1665) revealed the microscopic structures of numerous biological and inorganic objects and became an important source of information for later studies. Aside from the body of detailed observations reported and depicted in Micrographia, the Preface is in itself an extraordinary document that exhibits Hooke's fertile mind, philosophical insights, and rare ability to look into the future.

Characterization of dominant and cellulolytic bacterial communities along the gut of silver carp Hypophthalmichthys molitrix during cyanobacterial blooms

NASA Astrophysics Data System (ADS)

Luo, Congqiang; Yi, Chunlong; Ni, Leyi; Guo, Longgen

2017-05-01

Silver carp is one of the most important planktivorous fish in Chinese aquaculture and plays a significant role controlling cyanobacterial blooms. A balanced gut microbiota is crucial for growth and health of the host because of its important roles in immune defense, digestion of complex carbohydrates, and production of enterocytes. In our study, the dominant bacterial and cellulolytic bacterial ( Clostridium I, Clostridium III, Clostridium XIVab, and Fibrobacter) communities in the contents and mucus of the silver carp gut (foregut, midgut, and hindgut) were analyzed by denaturing gradient gel electrophoresis and quantitative polymerase chain reaction (qPCR) analyses. The results revealed that the dominant and cellulolytic bacterial communities were significantly different among gut regions as well as in contents and mucus. Bacterial diversity and richness in contents and mucus increased along the gut and were higher in contents than those in local mucus. A sequence analysis of gut samples exhibited the conservative phylotypes of Proteobacteria, Actinobacteria, and Firmicutes. The gut of silver carp harbored an abundance of cellulolytic bacteria, particularly Clostridium XIV ab. The foregut segment had the highest proportions of the four cellulolytic bacteria, followed by the midgut and hindgut. However, the proportions of cellulolytic species in the silver carp gut was much lower than those in the terrestrial vertebrate gastrointestinal tract. We conclude that gut bacteria could help silver carp obtain energy from cyanobacteria, which may be why silver carp can maintain high growth rates during cyanobacterial blooms.

Melanomacrophage centers in kidney, spleen and liver: A toxic response in carp fish (Cyprinus carpio) exposed to mercury chloride

NASA Astrophysics Data System (ADS)

Tjahjaningsih, Wahju; Pursetyo, Kustiawan Tri; Sulmartiwi, Laksmi

2017-02-01

This study aims to determine the potential of melanomacrophage centers (MMCs) as a bioindicators of environment polluted with mercury chloride. This study used the carp fish that were kept in an environment that contained mercury chloride with a concentration of 0.01, 0.05 and 0.1 ppm for 21 days. The rate of accumulation of macrophages in the tissue of kidney, spleen and liver were measured by the activity of N-acetylglucosaminidase. The results showed that the MMCs in the spleen and liver tissue of the carp fish potential as the bio-indicators of polluted environment ≥0.1 ppm of mercury chloride. The increased in accumulation of macrophages found in the kidney tissue of carp fish exposed to mercury chloride concentration of 0.01, 0.05 and 0.1 ppm, although no significant difference with control (0 ppm). The suppressive effect of the accumulation of immune response showed at the carp fish liver tissue macrophages which were exposed to mercury chloride lower than carp fish that were not exposed.

Bio-Tribology Properties of Bionic Carp Scale Morphology on Ti6A14V Surface

NASA Astrophysics Data System (ADS)

Wang, W.; Y Wei, X.; Meng, K.; Zhong, L. H.; Wang, Y.; Yu, X. H.

2017-12-01

In order to improve the bio-tribology properties of Ti6A14V surface, the bionic carp scale appearance pattern on Ti6A14V surface was prepared by laser surface texturing technology. The ball-disc reciprocating linear tribological experiment under different lubricants with dry friction was carried out by MRTR multifunction friction and wear testing machine using ZrO2/Ti6A14V as friction pair. The wear scar morphology of the sample surface was observed by SEM. The results show that for dry friction, the friction factor of the bionic carp scale morphology Ti6A14V reduces by 0.23 than those without bionic carp scale morphology, a decline of 45%. Under different lubrication conditions, the friction factors of samples with the bionic carp scale are increased in varying degrees with the increase of size of bionic texturing. The friction factor with same specimen under different lubrication conditions according to the ascending order are 0.5g/dl of sodium hyaluronate +0.5g/dl-γglobulin and 0.5g/dl mixed aqueous solution of sodium hyaluronate solution and artificial saliva. The wear volume also showed a similar variation.

Asian Carp Survivability Experiments and Water Transport Surveys in the Illinois River. Volume 2

DTIC Science & Technology

2013-01-01

Reference 3)) have documented the egg and larval stages of grass, black, silver and bighead carps in the Yangtze River. This early paper also reported...rates at controlled tempera- tures to better understand the early egg and larval stages of silver and bighead carp. In the Yangtze River, after the...translated by Duane Chapman 2006, Gezhouba Water Control Project and four famous fishes in Yangtze River, Hubei Science and Technology Press, Wuhan

Jurassic onychites (arm hooks) from squid-like cephalopods from the Wessex Basin, southern England

NASA Astrophysics Data System (ADS)

Hart, Malcolm; Hughes, Zoe; Page, Kevin; Price, Gregory; Smart, Christopher

2017-04-01

Modern coleoid (squid-like) cephalopods have arms that carry arrays of both suckers and hardened, organic hooks. Fossil arm hooks have been known since the description of Sternberg in 1823, although he identified them as algal remains. During the twentieth century there have been a number of descriptions of hooks (Kulicki & Szaniawski, 1972; Clarke & Engeser, 1988). Kulicki & Szaniawski (1972) gave these 'morphotypes' names using a binomial classification though, with many lacking defined (and figured) holotypes and, in some cases, only one recorded specimen, some of their designations should be regarded as invalid. Some of their morphotypes have, however, been reported widely, from DSDP sites on the Falkland Plateau as well as New Zealand, Germany, Svalbard, Poland and the United Kingdom. Exceptional soft-bodied preservation of species such as Belemnotheutis antiquus (Pearce, 1847) from the Callovian-Oxfordian of Wiltshire (UK) and Clarkeiteuthis montefiore (Buckman, 1880) from the Sinemurian of Dorset (UK) has allowed the identification of the host animal of some morphotypes, though the majority remain un-attributable. In the Christian Malford lagerstätte (Upper Callovian) of Wiltshire large numbers of hooks (including forms described as Acanthuncus, Arites, Deinuncus, Falcuncus, Longuncus and Paraglycerites) are found associated with an abundance of statoliths (cephalopod 'ear bones') and macrofossil evidence of both belemnites and teuthids, some of which includes exceptional soft-bodied preservation (Wilby et al., 2004; Hart et al., 2016). Many of the hook types cannot, at present be assigned to known taxa of coleoid. In Belemnotheutis antiquus there appears to be one form of simple, slightly curved hook and their shape appears to remain constant throughout the Callovian -Kimmeridgian interval. In the Lias Group, hooks of Clarkeiteuthis are very different, with inflated, often bi-lobate bases and each arm often characterised by the presence of different shapes

The influence of hook type, angler experience, and fish size on injury rates and the duration of capture in an Alaskan catch-and-release rainbow trout fishery

USGS Publications Warehouse

Meka, Julie M.

2004-01-01

Owing to concerns about the high incidence of past hooking injuries in Alagnak River rainbow trout Oncorhynchus mykiss, fish were captured with spin- and fly-fishing gear with barbed and barbless circle and "J" hooks to determine gear types contributing to injury. Landing and hook removal times were measured for a portion of fish captured, and the anatomical hooking location, hooking scar locations, bleeding intensity, angler experience, and fish size were recorded for all captured fish. Approximately 62% of fish captured experienced at least one new hooking injury, and 29% of fish had at least one past hooking injury. Small fish sustained higher new injury and bleeding rates, but large fish had higher past injury rates. Injury rates were higher for barbed J hooks, barbed J hooks took longer to remove, and fish caught by spin-fishing were injured more frequently than fish caught by fly-fishing. Fewer fly-fishing-caught fish were injured using circle hooks, and circle hooks tended to hook fish in only one location, generally in the jaw. Barbed J hooks were more efficient at landing fish, and J hooks were more efficient at landing fish than circle hooks. Novice anglers injured proportionally more fish than experienced anglers, primarily during hook removal. Landing time was positively correlated with fish size, and experienced anglers took longer to land fish than novices because they captured larger fish. These results suggest that a reduction in hooking injuries may be achieved by using circle hooks as an alternative to J hooks and barbless J hooks to reduce injury and handling time, yet catch efficiency for both methods would be reduced. Although fish captured with barbless J hooks and circle hooks had fewer injuries, it is important to note that each hook type also caused significant injury, and angler education is recommended to promote proper hook removal techniques.

Bioaccumulation of polybrominated diphenyl ethers (PBDEs) in sediment aged for 2 years to carps (Cyprinus carpio)

NASA Astrophysics Data System (ADS)

Tian, S. Y.; Li, J. Y.; Jia, X. M.

2017-08-01

In order to understand the risk of polybrominated diphenyl ethers (PBDEs) existing in sediment for years, the accumulation of PBDEs in sediment aged for 2 years to fish was investigated. Simulated aquatic system microcosms were conducted with PBDE contaminated sediment aged for 2 years and carps were cultured in the microcosms for 20 days. PBDE concentrations in carp tissues were analyzed to estimate the bioavailability of aged PBDEs in carps. The main spiked PBDE congeners were detected in sediment even though the contaminated sediment was aged for 2 years. Similarly, the five PBDE (BDE-28, 47, 100, 153 and 154) congeners which probably were bioaccumulated by carp were detected in fish tissues, indicating that PBDEs could be bioaccumulated after aging for 2 years. The PBDEs distribution revealed that the concentrations of polybrominated diphenyl ethers in tissues of Cyprinus carpio is in this order of magnitude: gut > liver > gill > fillet. The PBDEs concentrations in fillet were as high as 67.9 ng/g dry wt, in which BDE-47 contributed almost 50% in profile.

Isolation of innate immune response genes, expression analysis, polymorphism identification and development of genetic marker for linkage analysis in common carp (Cyprinus carpio)

USDA-ARS?s Scientific Manuscript database

Common carp are economically important foodfish worldwide. Over the past few years, carp aquaculture has suffered from enormous losses to a disease caused by cyprinid herpesvirus 3 (CyHV-3). A recent study reported that common carp strains/crossbreds have differential resistance to CyHV-3, suggest...

78 FR 23864 - Fisheries of the Exclusive Economic Zone Off Alaska; Pacific Cod by Catcher/Processors Using Hook...

Federal Register 2010, 2011, 2012, 2013, 2014

2013-04-23

... Catcher/Processors Using Hook-and-line Gear in the Western Regulatory Area of the Gulf of Alaska AGENCY.../processors (C/Ps) using hook-and-line gear in the Western Regulatory Area of the Gulf of Alaska (GOA). This... catch apportioned to C/Ps using hook-and-line gear in the Western Regulatory Area of the GOA. DATES...

77 FR 11776 - Fisheries of the Exclusive Economic Zone Off Alaska; Pacific Cod by Catcher/Processors Using Hook...

Federal Register 2010, 2011, 2012, 2013, 2014

2012-02-28

.../Processors Using Hook-and-Line Gear in the Central Regulatory Area of the Gulf of Alaska AGENCY: National... (C/Ps) using hook-and-line gear in the Central Regulatory Area of the Gulf of Alaska (GOA). This... catch apportioned to C/Ps using hook-and-line gear in the Central Regulatory Area of the GOA. DATES...

G-protein coupled estrogen receptor (GPER) inhibits final oocyte maturation in common carp, Cyprinus carpio.

PubMed

Majumder, Suravi; Das, Sumana; Moulik, Sujata Roy; Mallick, Buddhadev; Pal, Puja; Mukherjee, Dilip

2015-01-15

GPR-30, now named as GPER (G protein-coupled estrogen receptor) was first identified as an orphan receptor and subsequently shown to be required for estrogen-mediated signaling in certain cancer cells. Later studies demonstrated that GPER has the characteristics of a high affinity estrogen membrane receptor on Atlantic croaker and zebra fish oocytes and mediates estrogen inhibition of oocyte maturation in these two distantly related teleost. To determine the broad application of these findings to other teleost, expression of GPER mRNA and its involvement in 17β-estradiol mediated inhibition of oocyte maturation in other cyprinid, Cyprinus carpio was investigated. Carp oocytes at pre-vitellogenic, late-vitellogenic and post-vitellogenic stages of development contained GPER mRNA and its transcribed protein with a maximum at late-vitellogenic oocytes. Ovarian follicular cells did not express GPER mRNA. Carp oocytes GPER mRNA was essentially identical to that found in other perciformes and cyprinid fish oocytes. Both spontaneous and 17,20β-dihydroxy-4-pregnen-3-one (17,20β-P)-induced oocyte maturation in carp was significantly decreased when they were incubated with either E2, or GPER agonist G-1. On the other hand spontaneous oocyte maturation was significantly increased when carp ovarian follicles were incubated with an aromatase inhibitor, fadrozole, GPER antagonist, G-15 and enzymatic removal of the ovarian follicle cell layers. This increase in oocyte maturation was partially reversed by co-treatment with E2. Consistent with previous findings with human and fish GPR30, E2 treatment in carp oocytes caused increase in cAMP production and simultaneously decrease in oocyte maturation, which was inhibited by the addition of 17,20β-P. The results suggest that E2 and GPER play a critical role in regulating re-entry in to meiotic cell cycle in carp oocytes. Copyright © 2014 Elsevier Inc. All rights reserved.

A Laboratory Investigation of the Suspension, Transport, and Settling of Silver Carp Eggs Using Synthetic Surrogates

PubMed Central

Garcia, Tatiana; Zuniga Zamalloa, Carlo; Jackson, P. Ryan; Murphy, Elizabeth A.; Garcia, Marcelo H.

2015-01-01

Asian carp eggs are semi-buoyant and must remain suspended in the water to survive, supported by the turbulence of the flow, until they hatch and develop the ability to swim. Analysis of the transport and dispersal patterns of Asian carp eggs will facilitate the development and implementation of control strategies to target the early life stages. Experimenting with Asian carp eggs is complicated due to practical issues of obtaining eggs in close proximity to experimental facilities and extensive handling of eggs tends to damage them. Herein, we describe laboratory experiments using styrene beads (4.85 mm diameter) as synthetic surrogate eggs to mimic the physical properties of water-hardened silver carp eggs. The first set of experiments was completed in a rectangular vertical column filled with salt water. The salinity of the water was adjusted in an iterative fashion to obtain a close approximation of the fall velocity of the styrene beads to the mean fall velocity of silver carp water-hardened eggs. The terminal fall velocity of synthetic eggs was measured using an image processing method. The second set of experiments was performed in a temperature-controlled recirculatory flume with a sediment bed. The flume was filled with salt water, and synthetic eggs were allowed to drift under different flow conditions. Drifting behavior, suspension conditions, and settling characteristics of synthetic eggs were observed. At high velocities, eggs were suspended and distributed through the water column. Eggs that touched the sediment bed were re-entrained by the flow. Eggs saltated when they touched the bed, especially at moderate velocities and with a relatively flat bed. At lower velocities, some settling of the eggs was observed. With lower velocities and a flat bed, eggs were trapped near the walls of the flume. When bedforms were present, eggs were trapped in the lee of the bedforms in addition to being trapped near the flume walls. Results of this research study

A laboratory investigation of the suspension, transport, and settling of silver carp eggs using synthetic surrogates

USGS Publications Warehouse

Garcia, Tatiana; Zuniga Zamalloa, Carlo; Jackson, P. Ryan; Murphy, Elizabeth A.; Garcia, Marcelo H.

2015-01-01

Asian carp eggs are semi-buoyant and must remain suspended in the water to survive, supported by the turbulence of the flow, until they hatch and develop the ability to swim. Analysis of the transport and dispersal patterns of Asian carp eggs will facilitate the development and implementation of control strategies to target the early life stages. Experimenting with Asian carp eggs is complicated due to practical issues of obtaining eggs in close proximity to experimental facilities and extensive handling of eggs tends to damage them. Herein, we describe laboratory experiments using styrene beads (4.85 mm diameter) as synthetic surrogate eggs to mimic the physical properties of water-hardened silver carp eggs. The first set of experiments was completed in a rectangular vertical column filled with salt water. The salinity of the water was adjusted in an iterative fashion to obtain a close approximation of the fall velocity of the styrene beads to the mean fall velocity of silver carp water-hardened eggs. The terminal fall velocity of synthetic eggs was measured using an image processing method. The second set of experiments was performed in a temperature-controlled recirculatory flume with a sediment bed. The flume was filled with salt water, and synthetic eggs were allowed to drift under different flow conditions. Drifting behavior, suspension conditions, and settling characteristics of synthetic eggs were observed. At high velocities, eggs were suspended and distributed through the water column. Eggs that touched the sediment bed were re-entrained by the flow. Eggs saltated when they touched the bed, especially at moderate velocities and with a relatively flat bed. At lower velocities, some settling of the eggs was observed. With lower velocities and a flat bed, eggs were trapped near the walls of the flume. When bedforms were present, eggs were trapped in the lee of the bedforms in addition to being trapped near the flume walls. Results of this research study

Identification and characterization of MAVS from black carp Mylopharyngodon piceus.

PubMed

Zhou, Wei; Zhou, Jujun; Lv, Ying; Qu, Yixiao; Chi, Mengdie; Li, Jun; Feng, Hao

2015-04-01

MAVS (mitochondria antiviral signaling protein) plays an important role in the host cellular innate immune response against microbial pathogens. In this study, MAVS has been cloned and characterized from black carp (Mylopharyngodon piceus). The full-length cDNA of black carp MAVS (bcMAVS) consists of 2352 nucleotides and the predicted bcMAVS protein contains 579 amino acids. Structural analysis showed that bcMAVS is composed of functional domains including an N-terminal CARD, a central proline-rich domain, a putative TRAF2-binding motif and a C-terminal TM domain, which is similar to mammalian MAVS. bcMAVS is constitutively transcribed in all the selected tissues including gill, kidney, heart, intestine, liver, muscle, skin and spleen; bcMAVS mRNA level in intestine, liver, muscle increased but decreased in spleen right after GCRV or SVCV infection. Multiple bands of bcMAVS were detected in western blot when it was expressed in tissue culture, which is similar to mammalian MAVS. Immunofluorescence assay determined that bcMAVS is a mitochondria protein and luciferase reporter assay demonstrated that bcMAVS could induce zebrafish IFN and EPC IFN expression in tissue culture. Data generated in this manuscript has built a solid foundation for further elucidating the function of bcMAVS in the innate immune system of black carp. Copyright © 2015 Elsevier Ltd. All rights reserved.

Energetic consequences of an inducible morphological defence in crucian carp.

PubMed

Pettersson, Lars B; Brönmark, Christer

1999-10-01

Crucian carp (Carassius carassius) increases in body depth in response to chemical cues from piscivores and the deeper body constitutes a morphological defence against gape-limited piscivores. In the field, deep-bodied individuals suffer a density-dependent cost when competing with shallow-bodied conspecifics. Here, we use hydrodynamic theory and swimming respirometry to investigate the proposed mechanism underlying this effect, high drag caused by the deep-bodied morphology. Our study confirms that drag is higher for deep-bodied crucian carp, both in terms of estimated theoretical drag and power curve steepness. However, deep-bodied fish swimming at the velocity associated with minimum cost of transport, U mc , did not experience higher costs of transport than shallow-bodied fish. Deep-bodied crucian carp had significantly lower standard metabolic rates, i.e. metabolic rates at rest, and also lower U mc , and the resulting costs of transport were similar for the two morphs. Nevertheless, when deep-bodied individuals deviate from U mc , e.g. when increasing foraging effort under competition, their steeper power curves will cause substantial energy costs relative to shallow-bodied conspecifics. Furthermore, there is evidence that reductions in standard metabolic rate incur costs in terms of lower stress tolerance, reduced growth rate, and life history changes. Thus, this work provides links between hydrodynamics, a cost-reducing mechanism, and a density-dependent fitness cost associated with an inducible defence.

Anharmonic Vibrations of an "Ideal" Hooke's Law Oscillator

ERIC Educational Resources Information Center

Thomchick, John; McKelvey, J. P.

1978-01-01

Presents a model describing the vibrations of a mass connected to fixed supports by "ideal" Hooke's law springs which may serve as a starting point in the study of the properties of irons in a crystal undergoing soft mode activated transition. (SL)

A double standard for "Hooking Up": How far have we come toward gender equality?

PubMed

Allison, Rachel; Risman, Barbara J

2013-09-01

While sexual attitudes have liberalized in the past half century, research is mixed as to whether attitudes have become less gendered over time. Recent studies on college students' sexual and romantic relationships suggest that a sexual double standard continues to organize sexuality on many campuses. Data from the Online College Social Life Survey shed light on students' evaluation of casual sex, or "hooking up." In addition to exploring gendered attitudinal patterns, we use gender structure theory to explore how individual characteristics and normative expectations of campus group affiliations shape attitudes. While three quarters of students do not hold different standards for men and women's hooking up, attitudes are more conservative than liberal, with almost half of students losing respect for men and women who hook up "a lot." However, men are more likely to hold a traditional double standard, while women are more likely to espouse egalitarian conservative attitudes. Individual characteristics, including age, religion, race, social class and sexual orientation are frequently related to sexual attitudes, as are number of hook ups, fraternity/sorority affiliation and varsity athletic participation. Published by Elsevier Inc.

Arthroscopic evaluation for omalgia patients undergoing the clavicular hook plate fixation of distal clavicle fractures

PubMed Central

2014-01-01

Background The aim of this study is to investigate the anatomic changes in the shoulder joints responsible for omalgia after the clavicular hook plate fixation under arthroscope. Methods Arthroscopic examination was carried out for 12 omalgia patients who underwent clavicular hook plate fixation due to distal clavicle fractures. Functional outcome of shoulder was measured by the Japanese Orthopaedic Association (JOA) score before and after the withdrawal of the fixation plate. Results The rotator cuff compression by the clavicular hook was arthroscopically observed in 11 of the 12 cases. The JOA scores of the shoulder were significantly improved at 1 month after the withdrawal of the fixation plate (pain, 28 ± 2.4 vs. 15 ± 5.2; function, 19.2 ± 1.0 vs. 11.7 ± 1.9; range of movements, 26.8 ± 2.6 vs. 14.8 ± 3.4) compared with before. Conclusions The impingement of the hook to the rotator cuff may be the main cause for the omalgia. The appropriate hook and plate that fit to the curve of the clavicle as well as the acromion are necessary to decrease the severity of omalgia. PMID:24917508

The influence of pornography on sexual scripts and hooking up among emerging adults in college.

PubMed

Braithwaite, Scott R; Coulson, Gwen; Keddington, Krista; Fincham, Frank D

2015-01-01

The explosive growth in access to the Internet has led to a commensurate increase in the availability, anonymity, and affordability of pornography. An emerging body of research has shown associations between pornography and certain behaviors and attitudes; yet, how pornography actually influences these outcomes has not been documented. In two studies (Study 1 N = 969; Study 2 N = 992) we examined the hypothesis that pornography influences potentially risky sexual behavior (hooking up) among emerging adults via sexual scripts. Our results demonstrate that more frequent viewing of pornography is associated with a higher incidence of hooking up and a higher number of unique hook up partners. We replicated these effects both cross-sectionally and longitudinally while accounting for the stability of hook ups over the course of an academic semester. We also demonstrated that more frequent viewing of pornography is associated with having had more previous sexual partners of all types, more one occasion sexual partners ("one night stands"), and plans to have a higher number of sexual partners in the future. Finally, we provided evidence that more permissive sexual scripts mediated the association between more frequent pornography viewing and hooking up. We discuss these findings with an eye toward mitigating potential personal and public health risks among emerging adults.

Stimulation of glycerol kinase in grass carp preadipocytes by EPA.

PubMed

Lei, Caixia; Tian, Jingjing; Ji, Hong

2017-06-01

This study was conducted to assess the effect of eicosapentaenoic acid (EPA) on grass carp preadipocyte glycerol kinase (GyK) expression, as well as to explore the mechanism. Here, we cloned partial sequence of grass carp GyK gene and analyzed its tissue distribution. The result showed that GyK gene expressed most in the liver, followed by adipose tissue and the kidney. Besides, 400 μM oleic acid (18:1n-9, OA) was used to establish a hypertrophic preadipocyte model. GyK gene expression and enzyme activity were significantly enhanced after model cells were treated with 100 μM eicosapentaenoic acid (20:5n-3, EPA) for 6, 12, and 24 h. Meanwhile, peroxisome proliferative-activated receptor (PPAR)γ, adipose triglyceride lipase (ATGL), and the two isoforms of grass carp HSL gene were first identified by Sun et al (2016), and they defined the two isoforms as HSLa and HSLb. Therefore, maybe HSLa and HSLb are appropriate.. The content of triglyceride was dramatically increased by EPA treatment for 24 h. Further, a competitive ATGL antagonist, HY-15859, attenuated the increase in GyK induced by EPA at 12 h. Surprisingly, the enhanced lipolysis and PPARγ gene expression induced by serum deprivation were paralleled by an increase in GyK gene expression, whereas a stabilization in GyK enzyme activity. Other fatty acids, including docosahexaenoic acid, alpha-linolenic acid, linoleic acid, and OA also promoted GyK gene expression. Moreover, an irreversible PPARγ antagonist, GW9662, was used to investigate the role of PPARγ in GyK induction. Data showed that GW9662 abolished the induction of GyK by EPA at 12 h. Together, these data suggested that EPA elevated grass carp preadipocytes GyK expression. ATGL and PPARγ contributed to the induction of GyK. PPARγ may be a key regulator in response to GyK expression induced by EPA.

Human consumption as an invasive species management strategy. A preliminary assessment of the marketing potential of invasive Asian carp in the US.

PubMed

Varble, Sarah; Secchi, Silvia

2013-06-01

Over the past 20 years, Asian carp have invaded rivers and lakes in the Midwest and southern United States, with large negative impacts, such as encroachment on the habitat of native fish and mass die-off. They also respond to boat motors by jumping out of the water, which can cause harm to boaters and fishermen. Policymakers in the Great Lakes region between the US and Canada are worried about possible expansion of the Asian carp to their region and its effects on their fishing industry. A potential solution to the problem is to harvest Asian carp for human consumption. This study analyzes the results of the first national survey on the attitudes of US fish consumers towards Asian carp. We find that this is a potentially promising strategy. Most respondents would be willing to try a free sample of Asian carp and would be willing to pay for it. Because of the negative connotation attached to carp in general, this figure is encouraging. Creating demand for Asian carp could be a market based, cost-effective solution for a problem (invasive species) that is typically dealt with through command and control policies, if it is coupled with appropriate policies and safeguards to ensure the fish is eventually eradicated and not cultivated for profit after removal from US rivers and lakes. Copyright © 2013 Elsevier Ltd. All rights reserved.

[Complications of treatment of acromioclavicular joint dislocation and unstable distal clavicular fracture with clavicular hook plate].

PubMed

Zhu, Yi-Yong; Cui, Heng-Yan; Jiang, Pan-Qiang; Wang, Jian-Liang

2013-11-01

To investigate the causes and prevention of the complications about treatment of acromioclavicular joint dislocation (Tossy III) and unstable distal clavicular fracture (Neer II) with clavicular hook plate. From January 2001 to December 2011, 246 patients with acromioclavicular joint dislocation (Tossy III) and 222 patients with unstable distal clavicular fracture (Neer II) were treated with acromioclvicular hook plate fixation,including 348 males and 120 females with an average age of 45.4 years old ranging from 21 to 80 years old. The mean time from injury to operation was 30.8 hours (ranged from 1 h to 15 d). All patients had normal shoulder function before injury. According to Karlsson evaluation standard, the cases with excellent and good function of the shoulder joint were regarded as the normal group, and the cases with poor function of shoulder joint as the abnormal group. The comparison of the range of forward flexion,backward stretch, adduction, abduction and elevation of shoulder joints between two groups was performed. The data of impingement, subacromial osteolysis, acromioclavicular arthritis, clavicular stress fracture, downward acromioclavicular joint subluxation, hook cut-out and hook break were summarized. All patients were followed up from 8 to 48 months with an average of 12.5 months. The results were excellent in 308 cases,good in 76,and poor in 84 according to Karlsson evaluation. The excellent and good rate was 82.1%. The difference of the range of forward flexion, backward stretch, adduction, abduction and elevation of shoulder joints between two groups had a statistically significant difference (P < 0.01). Among 84 poor cases, there were 41 (8.76%) in acromial impingement or inadequate place of plate hook, 12 (2.56%) with subacromial osteolysis or/and bursitis, 10 (2.14%) with acromioclavicular arthritis or painful shoulder caused by delayed dirigation,7 (1.50%) with clavicular stress fracture or interal plate upward, 6 (1.28%) with

Development of a Fluvial Egg Drift Simulator to evaluate the transport and dispersion of Asian carp eggs in rivers

USGS Publications Warehouse

Garcia, Tatiana; Jackson, P. Ryan; Murphy, Elizabeth A.; Valocchi, Albert J.; Garcia, Marcelo H.

2013-01-01

Asian carp are migrating towards the Great Lakes and are threatening to invade this ecosystem, hence there is an immediate need to control their population. The transport of Asian carp eggs in potential spawning rivers is an important factor in its life history and recruitment success. An understanding of the transport, development, and fate of Asian carp eggs has the potential to create prevention, management, and control strategies before the eggs hatch and develop the ability to swim. However, there is not a clear understanding of the hydrodynamic conditions at which the eggs are transported and kept in suspension. This knowledge is imperative because of the current assumption that suspension is required for the eggs to survive. Herein, FluEgg (Fluvial Egg Drift Simulator), a three-dimensional Lagrangian model capable of evaluating the influence of flow velocity, shear dispersion and turbulent diffusion on the transport and dispersal patterns of Asian carp eggs is presented. The model's variables include not only biological behavior (growth rate, density changes) but also the physical characteristics of the flow field, such as mean velocities and eddy diffusivities. The performance of the FluEgg model was evaluated using observed data from published flume experiments conducted in China with water-hardened Asian carp eggs as subjects. FluEgg simulations show a good agreement with the experimental data. The model was also run with observed data from the Sandusky River in Ohio to provide a real-world demonstration case. This research will support the identification of critical hydrodynamic conditions (e.g., flow velocity, depth, and shear velocity) to maintain eggs in suspension, assist in the evaluation of suitable spawning rivers for Asian carp populations and facilitate the development of prevention, control and management strategies for Asian carp species in rivers and water bodies.

Molecular Characterization, Tissue Distribution and Expression, and Potential Antiviral Effects of TRIM32 in the Common Carp (Cyprinus carpio).

PubMed

Wang, Yeda; Li, Zeming; Lu, Yuanan; Hu, Guangfu; Lin, Li; Zeng, Lingbing; Zhou, Yong; Liu, Xueqin

2016-10-09

Tripartite motif-containing protein 32 (TRIM32) belongs to the tripartite motif (TRIM) family, which consists of a large number of proteins containing a RING (Really Interesting New Gene) domain, one or two B-box domains, and coiled coil motif followed by different C-terminal domains. The TRIM family is known to be implicated in multiple cellular functions, including antiviral activity. However, it is presently unknown whether TRIM32 of common carp ( Cyprinus carpio ) has the antiviral effect. In this study, the sequence, expression, and antiviral function of TRIM32 homolog from common carp were analyzed. The full-length coding sequence region of trim32 was cloned from common carp. The results showed that the expression of TRIM32 (mRNA) was highest in the brain, remained stably expressed during embryonic development, and significantly increased following spring viraemia of carp virus (SVCV) infection. Transient overexpression of TRIM32 in affected Epithelioma papulosum cyprinid cells led to significant decrease of SVCV production as compared to the control group. These results suggested a potentially important role of common carp TRIM32 in enhancing host immune response during SVCV infection both in vivo and in vitro.

Molecular Characterization, Tissue Distribution and Expression, and Potential Antiviral Effects of TRIM32 in the Common Carp (Cyprinus carpio)

PubMed Central

Wang, Yeda; Li, Zeming; Lu, Yuanan; Hu, Guangfu; Lin, Li; Zeng, Lingbing; Zhou, Yong; Liu, Xueqin

2016-01-01

Tripartite motif-containing protein 32 (TRIM32) belongs to the tripartite motif (TRIM) family, which consists of a large number of proteins containing a RING (Really Interesting New Gene) domain, one or two B-box domains, and coiled coil motif followed by different C-terminal domains. The TRIM family is known to be implicated in multiple cellular functions, including antiviral activity. However, it is presently unknown whether TRIM32 of common carp (Cyprinus carpio) has the antiviral effect. In this study, the sequence, expression, and antiviral function of TRIM32 homolog from common carp were analyzed. The full-length coding sequence region of trim32 was cloned from common carp. The results showed that the expression of TRIM32 (mRNA) was highest in the brain, remained stably expressed during embryonic development, and significantly increased following spring viraemia of carp virus (SVCV) infection. Transient overexpression of TRIM32 in affected Epithelioma papulosum cyprinid cells led to significant decrease of SVCV production as compared to the control group. These results suggested a potentially important role of common carp TRIM32 in enhancing host immune response during SVCV infection both in vivo and in vitro. PMID:27735853

Intraspecific Scaling of the Resting and Maximum Metabolic Rates of the Crucian Carp (Carassius auratus)

PubMed Central

Huang, Qingda; Zhang, Yurong; Liu, Shuting; Wang, Wen; Luo, Yiping

2013-01-01

The question of how the scaling of metabolic rate with body mass (M) is achieved in animals is unresolved. Here, we tested the cell metabolism hypothesis and the organ size hypothesis by assessing the mass scaling of the resting metabolic rate (RMR), maximum metabolic rate (MMR), erythrocyte size, and the masses of metabolically active organs in the crucian carp (Carassius auratus). The M of the crucian carp ranged from 4.5 to 323.9 g, representing an approximately 72-fold difference. The RMR and MMR increased with M according to the allometric equations RMR = 0.212M 0.776 and MMR = 0.753M 0.785. The scaling exponents for RMR (b r) and MMR (b m) obtained in crucian carp were close to each other. Thus, the factorial aerobic scope remained almost constant with increasing M. Although erythrocyte size was negatively correlated with both mass-specific RMR and absolute RMR adjusted to M, it and all other hematological parameters showed no significant relationship with M. These data demonstrate that the cell metabolism hypothesis does not describe metabolic scaling in the crucian carp, suggesting that erythrocyte size may not represent the general size of other cell types in this fish and the metabolic activity of cells may decrease as fish grows. The mass scaling exponents of active organs was lower than 1 while that of inactive organs was greater than 1, which suggests that the mass scaling of the RMR can be partly due to variance in the proportion of active/inactive organs in crucian carp. Furthermore, our results provide additional evidence supporting the correlation between locomotor capacity and metabolic scaling. PMID:24376588

Automation Hooks Architecture for Flexible Test Orchestration - Concept Development and Validation

NASA Technical Reports Server (NTRS)

Lansdowne, C. A.; Maclean, John R.; Winton, Chris; McCartney, Pat

2011-01-01

The Automation Hooks Architecture Trade Study for Flexible Test Orchestration sought a standardized data-driven alternative to conventional automated test programming interfaces. The study recommended composing the interface using multicast DNS (mDNS/SD) service discovery, Representational State Transfer (Restful) Web Services, and Automatic Test Markup Language (ATML). We describe additional efforts to rapidly mature the Automation Hooks Architecture candidate interface definition by validating it in a broad spectrum of applications. These activities have allowed us to further refine our concepts and provide observations directed toward objectives of economy, scalability, versatility, performance, severability, maintainability, scriptability and others.

Use of the S-hook for Pelvic Fixation in Rib-Based Treatment of Early-Onset Scoliosis: A Multicenter Study.

PubMed

Ramirez, Norman; Flynn, John M; Smith, John T; Vitale, Michael; Sturm, Peter F; DʼAmato, Charles; Samdani, Amer; Machiavelli, Raul; El-Hawary, Ron

2015-06-01

Retrospective review. The purpose of this study was to evaluate how several preoperative variables affect the outcome using the rib-to-pelvis S-hook constructs of a rib-based distraction implant (Vertical Expandable Prosthetic Titanium Rib). Rib-to-pelvis fixation with S-hooks is one of the options for distal anchoring of rib-based distraction growing rod construct to control early-onset spinal deformity. Since the initial report, the indications of pelvic fixation with S-hooks have been extended and modified. This is an institutional review board-approved retrospective study of patients who underwent rib-based growing rod system surgery-rib-to-pelvis construct with Dunn-McCarthy S-hook. Data evaluation included history, physical examination, preoperative and postoperative radiographs, surgical variables, and complications. Sixty-five patients were evaluated; 38 were male and 27 were female. Mean age at initial procedure was 71 months. The mean follow-up was 46 months. There was a statistically significant improvement of the immediate postoperative Cobb angle and the last follow-up Cobb angle (P < 0.0001). Fifty percent of the patients (32/65) had S-hook-related complications. The most common complication was sliding of the S-hook out of the iliac crest, followed by infection, neuropathic pain, distal migration of more than 2 cm, fracture of the hook, and bursitis. The complications were related to the preoperative ambulatory status, the use of end-to-end rod connectors, surgical time, and not positioning the hook over the central one-third of the iliac crest at the initial implantation. The use of the S-hook as a pelvic attachment of the rib-based system is indicated in nonambulatory patients with progressive, early-onset scoliosis curve with a lack of adequate anchor at the lumbar spine. Several technical factors should be considered to reduce the complication rate. 3.

Astronaut James Newman with latch hook for tether device

NASA Technical Reports Server (NTRS)

1993-01-01

Astronaut James H. Newman, mission specialist, shows off a latch hook for a tether device used during the STS-51 extravehicular activity (EVA) on September 16, 1993. Newman, on Discovery's middeck, appears surrounded by sleep restraints.

Pathogenesis of acute viral disease induced in fish by carp interstitial nephritis and gill necrosis virus.

PubMed

Pikarsky, Eli; Ronen, Ariel; Abramowitz, Julia; Levavi-Sivan, Berta; Hutoran, Marina; Shapira, Yechiam; Steinitz, Michael; Perelberg, Ayana; Soffer, Dov; Kotler, Moshe

2004-09-01

A lethal disease of koi and common carp (species Cyprinus carpio) has afflicted many fish farms worldwide since 1998, causing severe financial losses. Morbidity and mortality are restricted to common carp and koi and appear in spring and autumn, when water temperatures are 18 to 28 degrees C. We have isolated the virus causing the disease from sick fish, propagated it in koi fin cell culture, and shown that virus from a single clone causes lethal disease in carp and koi upon infection. Intraperitoneal virus injection or bathing the fish in virus-containing water kills 85 to 100% of the fish within 7 to 21 days. This virus is similar to the previously reported koi herpesvirus; however, it has characteristics inconsistent with the herpesvirus family, and thus we have called it carp interstitial nephritis and gill necrosis virus. We examined the pathobiology of this disease in carp by using immunohistochemistry and PCR. We found large amounts of the virus in the kidneys of sick fish and smaller amounts in liver and brain. A rapid increase in the viral load in the kidneys was detected by using both immunofluorescence and semiquantitative PCR. Histological analyses of fish at various times after infection revealed signs of interstitial nephritis as early as 2 days postinfection, which increased in severity up to 10 days postinfection. There was severe gill disease evidenced by loss of villi with accompanying inflammation in the gill rakers. Minimal focal inflammation was noted in livers and brains. This report describes the etiology and pathology of a recently described viral agent in fish.

Pathogenesis of Acute Viral Disease Induced in Fish by Carp Interstitial Nephritis and Gill Necrosis Virus

PubMed Central

Pikarsky, Eli; Ronen, Ariel; Abramowitz, Julia; Levavi-Sivan, Berta; Hutoran, Marina; Shapira, Yechiam; Steinitz, Michael; Perelberg, Ayana; Soffer, Dov; Kotler, Moshe

2004-01-01

A lethal disease of koi and common carp (species Cyprinus carpio) has afflicted many fish farms worldwide since 1998, causing severe financial losses. Morbidity and mortality are restricted to common carp and koi and appear in spring and autumn, when water temperatures are 18 to 28°C. We have isolated the virus causing the disease from sick fish, propagated it in koi fin cell culture, and shown that virus from a single clone causes lethal disease in carp and koi upon infection. Intraperitoneal virus injection or bathing the fish in virus-containing water kills 85 to 100% of the fish within 7 to 21 days. This virus is similar to the previously reported koi herpesvirus; however, it has characteristics inconsistent with the herpesvirus family, and thus we have called it carp interstitial nephritis and gill necrosis virus. We examined the pathobiology of this disease in carp by using immunohistochemistry and PCR. We found large amounts of the virus in the kidneys of sick fish and smaller amounts in liver and brain. A rapid increase in the viral load in the kidneys was detected by using both immunofluorescence and semiquantitative PCR. Histological analyses of fish at various times after infection revealed signs of interstitial nephritis as early as 2 days postinfection, which increased in severity up to 10 days postinfection. There was severe gill disease evidenced by loss of villi with accompanying inflammation in the gill rakers. Minimal focal inflammation was noted in livers and brains. This report describes the etiology and pathology of a recently described viral agent in fish. PMID:15308746

Pre-operative localization of solitary pulmonary nodules with computed tomography-guided hook wire: report of 181 patients.

PubMed

Hanauer, Matthieu; Perentes, Jean Yannis; Krueger, Thorsten; Ris, Hans-Beat; Bize, Pierre; Schmidt, Sabine; Gonzalez, Michel

2016-01-16

Video-assisted thoracic surgery (VATS) is currently performed to diagnose and treat solitary pulmonary nodules (SPN). However, the intra-operative identification of deep nodules can be challenging with VATS as the lung is difficult to palpate. The aim of the study was to report the utility and the results of pre-operative computed tomography (CT)-guided hook wire localization of SPN. All records of the patients undergoing CT-guided hook wire localization prior to VATS resection for SPN between 2002 and 2013 were reviewed. The efficacy in localizing the nodule, hook wire complications, necessity to convert VATS to thoracotomy and the histology of SPN are reported. One hundred eighty-one patients (90 females, mean age 63 y, range 28-82 y) underwent 187 pulmonary resections after CT-guided hook wire localization. The mean SPN diameter was 10.3 mm (range: 4-29 mm). The mean distance of the lesion from the pleural surface was 11.6 mm (range: 0-45 mm). The mean time interval from hook wire insertion to VATS resection was 224 min (range 54-622 min). Hook wire complications included pneumothorax requiring chest tube drainage in 4 patients (2.1%) and mild parenchymal haemorrhage in 11 (5.9%) patients. Migration of the hook wire occurred in 7 patients (3.7%) although it did not affect the success of VATS resection (nodule location guided by the lung puncture site). Three patients underwent additional wedge resection by VATS during the same procedure because no lesion was identified in the surgical specimen. Conversion thoracotomy was required in 13 patients (7 %) for centrally localized lesions (6 patients) and pleural adhesions (7 patients). The mean operative time was 60 min (range 18-135 min). Pathological examination revealed a malignant lesion in 107 patients (59 %). The diagnostic yield was 98.3 %. VATS resection for SPN after CT-guided hook wire localization for SPN is safe and allows for proper diagnosis with a low thoracotomy conversion rate.

Historical demography of common carp estimated from individuals collected from various parts of the world using the pairwise sequentially markovian coalescent approach.

PubMed

Yuan, Zihao; Huang, Wei; Liu, Shikai; Xu, Peng; Dunham, Rex; Liu, Zhanjiang

2018-04-01

The inference of historical demography of a species is helpful for understanding species' differentiation and its population dynamics. However, such inference has been previously difficult due to the lack of proper analytical methods and availability of genetic data. A recently developed method called Pairwise Sequentially Markovian Coalescent (PSMC) offers the capability for estimation of the trajectories of historical populations over considerable time periods using genomic sequences. In this study, we applied this approach to infer the historical demography of the common carp using samples collected from Europe, Asia and the Americas. Comparison between Asian and European common carp populations showed that the last glacial period starting 100 ka BP likely caused a significant decline in population size of the wild common carp in Europe, while it did not have much of an impact on its counterparts in Asia. This was probably caused by differences in glacial activities in East Asia and Europe, and suggesting a separation of the European and Asian clades before the last glacial maximum. The North American clade which is an invasive population shared a similar demographic history as those from Europe, consistent with the idea that the North American common carp probably had European ancestral origins. Our analysis represents the first reconstruction of the historical population demography of the common carp, which is important to elucidate the separation of European and Asian common carp clades during the Quaternary glaciation, as well as the dispersal of common carp across the world.

Bioaccumulation of PCB Contaminants in Five Fish Species in Utah Lake as Affected by Carp Removal

NASA Astrophysics Data System (ADS)

Sanjinez-Guzmán, V. A.; Cadet, E. L.; Crandall, T.; Chamberlain, T.; Rakotoarisaona, H.; Morris, P.

2017-12-01

State reports published by the Utah Department of Health (2005) and the Utah Department of Water Quality (2008) determined that there were elevated levels of PCBs (Polychlorinated biphenyls) that exceeded the EPA's cancer (0.02 𝑚𝑔 𝑘𝑔-1) and non-cancer screening levels (0.08 𝑚𝑔 𝑘𝑔-1) in two fish species from Utah Lake, the Common Carp (Cyprinus carpio) and the Channel Catfish (Ictalurus punctatus). Fish consumption advisories were issued for both of these fish species due to their health effects of PCBs. The Common Carp is a non-native predatory species that comprise 90% of the biomass in Utah Lake. As of September 2009, an extensive carp removal program was instituted by the Department of Natural Resources and began the removal of 75% of the carp population. The purpose of this study is to assess the impact of carp removal on PCB levels in five sport fish species consumed by Utah citizens. The fish being analyzed are the Common Carp (Cyprinus carpio), Channel Catfish (Ictalurus punctatus), Black Bullhead (Ameiurus melas), Walleye (Sander vitreus), and White Bass (Morone chrysops). One-hundred twenty (120) fish were collected from Utah Lake and subcategorized by their gender, tissue type (fillet and offal), weight, and size: small (under 33 cm), medium (33 cm - 43 cm), and large (greater than 43 cm). This was done in order to determine the variation of contaminant levels in each subcategory. PCB analysis was performed by Utility Testing Laboratory in Salt Lake City, Utah. Results show there has been a significant increase in PCB levels in all fish species in comparison with the state reports (2008). All fish species have exceeded the EPA cancer screening level, except for the fillet tissue of the White Bass species. In Common Carp fillet, and offal decreased concentrations of 11.80% and 23.72%, respectively. In Channel catfish: the PCB levels in the fillet increase by 87.93%, however, the offal levels

Organophosphate and carbamate insecticides in agricultural waters and cholinesterase (ChE) inhibition in common carp (Cyprinus carpio)

USGS Publications Warehouse

Gruber, S.J.; Munn, M.D.

1998-01-01

Cholinesterase (ChE) activity was used as a biomarker for assessing exposure of common carp (Cyprinus carpio) to organophosphate and carbamate insecticides from irrigated agricultural waters. Carp were collected from a lake (Royal Lake) that receives most of its water from irrigation return flows and from a reference lake (Billy Clapp Lake) outside of the irrigation system. Results indicated that the mean whole-brain ChE activity of carp from Royal Lake (3.47 μmol/min/g tissue) was 34.2% less than that of carp from Billy Clapp Lake (5.27 μmol/min/g tissue) (p = 0.003). The depressed ChE activity in brain tissue of Royal Lake carp was in response to ChE-inhibiting insecticides detected in water samples in the weeks prior to tissue sampling; the most frequently detected insecticides included chlorpyrifos, azinphos-methyl, carbaryl, and ethoprop. Neither sex nor size appears to be a covariable in the analysis; ChE activity was not correlated with fish length or weight in either lake and there was no significant difference in ChE activity between the two sexes within each lake. Although organophosphate and carbamate insecticides can break down rapidly in the environment, this study suggests that in agricultural regions where insecticides are applied for extended periods of the year, nontarget aquatic biota may be exposed to high levels of ChE-inhibiting insecticides for a period of several months.

Response of the intestinal mucosal barrier of carp (Cyprinus carpio) to a bacterial challenge by Aeromonas hydrophila intubation after feeding with β-1,3/1,6-glucan.

PubMed

Jung-Schroers, V; Adamek, M; Harris, S; Syakuri, H; Jung, A; Irnazarow, I; Steinhagen, D

2018-03-15

The effect of dietary β-glucan on the bacterial community in the gut of common carp (Cyprinus carpio) was examined after oral application of Aeromonas hydrophila. Carp received either feed supplemented with 1% MacroGard ® , a β-1,3/1,6-glucan, or a β-glucan-free diet. Fourteen days after feeding, half of the carp from each group were intubated with 10 9 colony-forming units (CFU) of a pathogenic strain of A. hydrophila. Gut samples were taken 12 hr to 7 days after application and analysed using microbiological and molecular biological techniques (NGS, RT-PCR-DGGE). The reaction of the mucosa and the microbiota to an A. hydrophila intubation differed in carp fed with β-glucan compared to carp from the control group. In β-glucan fed carp, the total bacterial amount was lower but the number of bacterial species was higher. Bacterial composition was different for carp from both treatment groups. The number of mucin filled goblet cells was reduced in carp fed the β-glucan diet. Mucus was obviously released from the goblet cells and was probably washed out of the gut together with high numbers of bacteria. This might be protective against pathogenic bacteria and, therefore, feeding with β-glucan may provide protection against infections of the gut in carp. © 2018 John Wiley & Sons Ltd.

Evaluating upstream passage and timing of approach by adult bigheaded carps at a gated dam on the Illinois River

USGS Publications Warehouse

Lubejko, Matthew; Whitledge, Greg; Coulter, Alison A.; Brey, Marybeth; Oliver, Devon; Garvey, James E.

2017-01-01

Dams are a conservation threat because they function as barriers to native fish movement; however, they may prevent the spread of invasive species. Invasive bigheaded carps (Hypophthalmichthys spp.) threaten the Great Lakes ecosystem and are advancing towards Lake Michigan via the Illinois River. Navigation dams on the Illinois River may deter bigheaded carps' upstream movement. We investigated the permeability of the Starved Rock Lock and Dam (SRLD), the most downstream gated Illinois River dam, to bigheaded carps' migration by examining the timing of individuals approaching and passing through SRLD in relation to gate openness, tailwater elevation, and water temperature. Using acoustic telemetry of (N = ~104 per year) tagged fish, 13 upstream passages of bigheaded carps occurred through SRLD between 2013 and 2016. Eleven passages occurred through the dam gates and 2 through the lock chamber, indicating deterrents (e.g., CO2) placed in SRLD lock chamber may only limit passage of a small proportion of all fish passing through the lock-and-dam structure. Passages were documented only in 2013 and 2015. Most of the dam gate passages occurred during high water when gates were completely out of the water. Timing of bigheaded carps approaching SRLD was positively correlated with rising water temperature and high tailwater elevation, and all fish approached during late March through mid-September. Movement through dams is rare; modifying gate operations to reduce gate openness during late spring and summer could further reduce the permeability of gated dams such as SRLD to bigheaded carps, slowing their upstream advance.

Comparative nutritional value of Jatropha curcas protein isolate and soy protein isolate in common carp.

PubMed

Nepal, Sunil; Kumar, Vikas; Makkar, Harinder P S; Stadtlander, Timo; Romano, Nicholas; Becker, Klaus

2018-02-01

Jatropha seed cake (JSC) is an excellent source of protein but does contain some antinutritional factors (ANF) that can act as toxins and thus negatively affect the growth and health status of fish. While this can limit the use of JSC, detoxified Jatropha protein isolate (DJPI) may be a better option. An 8-week study was performed to evaluate dietary DJPI to common carp Cyprinus carpio. Five iso-nitrogenous diets (crude protein of 38%) were formulated that consisted of a C ontrol (fish meal (FM) based protein), J 50 or J 75 (50 and 75% of FM protein replaced by DJPI), and S 50 or S 75 (50 and 75% of FM protein replaced by soy protein isolate, SPI) and fed to triplicate groups of 75 carp fingerlings (75; av. wt. ± SD; 11.4 ± 0.25 g). The growth, feeding efficiencies, digestibility, plasma biochemistry, and intestinal enzymes were measured. Results showed that growth performance of fish fed the S 75 - or DJPI-based diets were not significantly different from those fed the C ontrol diet, while carp fed the S 50 had significantly better growth than the J 75 diet. Fish fed the J 75 diet had significantly lower protein and lipid digestibility as well as significantly lower intestinal amylase and protease activities than all other groups. However, all plant protein-based diets led to significantly higher crude protein, crude lipid, and gross energy in the body of common carp compared to the control treatment. Plasma cholesterol and creatinine significantly decreased in the plant protein fed groups, although plasma triglyceride as well as the red blood cells count, hematocrit, albumin, globulin, total plasma protein, and lysozyme activity were higher in plant protein fed groups compared to FM fed group. White blood cells, hemoglobulin concentration, alkaline phosphatase and alanine transaminase activities, and glucose level in blood did not differ significantly among treatments. The results suggest that the DJPI is non-toxic to carp and can be used to replace FM in

Optimization of gelatine extraction from grass carp (Catenopharyngodon idella) fish skin by response surface methodology.

PubMed

Kasankala, Ladislaus M; Xue, Yan; Weilong, Yao; Hong, Sun D; He, Qian

2007-12-01

To establish the optimum gelatine extraction conditions from grass carp fish skin, response surface methodology (RSM) was adopted in this study. The effects of concentration of HCl (%, A), pre-treatment time (h, B), extraction temperature ( degrees C, C) and extraction time (h, D) were studied. The responses were yield (%) and gel strength (g). A=1.19%, B=24 h, C=52.61 degrees C and D=5.12h were determined as the optimum conditions while the predicted responses were 19.83% yield and 267 g gel strength. Gelling and melting points were 19.5 degrees C and 26.8 degrees C, respectively. Moreover, grass carp gelatine showed high contents of imino acids (proline and hydroxyproline) 19.47%. RSM provided a powerful tool to optimize the extraction parameters and the results may be adapted for industrial extraction of gelatine from grass carp fish skins.

[A new type sternoclavicular hook plate for unstable sternoclavicular joint dislocation and fracture].

PubMed

Zhang, Chuan-Yi; Lin, Lie; Liang, Jun-Bo; Wang, Bin; Chen, Guo-Fu; Chen, Hai-Xiao

2016-11-25

To evaluate the therapeutic effect of a new type sternoclavicular hook plate fixation in treating unstable sternoclavicular joint dislocation and fracture. From June 2011 to December 2013, 32 patients with sternoclavicular joint dislocation and fracture were treated with a new type sternoclavicular hook plate fixation, including 24 males and 8 females with an average age of 42 years ranging from 25 to 76 years;12 patients were anterior dislocation, 5 pations were posterior dislocation, 10 patients were internal extremity of clavicle fracture and 5 patients were sternoclavicular joint dislocation combined with fracture. The anterior fracture dislocation of the sternoclavicular joint adopted standard sternoclavicular joint hook plate, and the posterior dislocation was at the distal end of the hook of the steel plate, that is, the front part of the handle of the breast was added with a nut and a gasket to prevent the re-dislocation after operation. The results were evaluated according to Rockwood score. No complication happened in all patients. X-ray and CT showed that the dislocation and fracture of the sternoclavicular joint was well reduced and the plate was on right position. All patients were followed up for 6 to 24 months with an average of 10 months. At 6 to 3 months after operation, the fracture was healing without re-dislocation of the sternoclavicular joint, the medial end of the clavicle anatomical structure were restored, functional satisfaction, in which 9 patients with the swelling around sternoclavicular joint, but no pain and other symptoms. The total Rockwood score was 12.78±1.43; the results were excellent in 24 cases, good in 8 cases. The use of the new type of locking hook plate for the treatment of unstable fracture of the sternoclavicular joint, internal fixation is reliable, high security, easy to operate, to provide a reliable method for the treatment of such trauma.

Using silver and bighead carp cell lines for the identification of a unique metabolite fingerprint from thiram-specific chemical exposure

USGS Publications Warehouse

Putnam, Joel G.; Nelson, Justine; Leis, Eric M; Erickson, Richard A.; Hubert, Terrance D.; Amberg, Jon J.

2017-01-01

Conservation biology often requires the control of invasive species. One method is the development and use of biocides. Identifying new chemicals as part of the biocide registration approval process can require screening millions of compounds. Traditionally, screening new chemicals has been done in vivo using test organisms. Using in vitro (e.g., cell lines) and in silico (e.g., computer models) methods decrease test organism requirements and increase screening speed and efficiency. These methods, however, would be greatly improved by better understanding how individual fish species metabolize selected compounds.We combined cell assays and metabolomics to create a powerful tool to facilitate the identification of new control chemicals. Specifically, we exposed cell lines established from bighead carp and silver carp larvae to thiram (7 concentrations) then completed metabolite profiling to assess the dose-response of the bighead carp and silver carp metabolome to thiram. Forty one of the 700 metabolomic markers identified in bighead carp exhibited a dose-response to thiram exposure compared to silver carp in which 205 of 1590 metabolomic markers exhibited a dose-response. Additionally, we identified 11 statistically significant metabolomic markers based upon volcano plot analysis common between both species. This smaller subset of metabolites formed a thiram-specific metabolomic fingerprint which allowed for the creation of a toxicant specific, rather than a species-specific, metabolomic fingerprint. Metabolomic fingerprints may be used in biocide development and improve our understanding of ecologically significant events, such as mass fish kills.

Are we sinking Sandy Hook? Possible impacts of groundwater extraction to sea-level change

NASA Astrophysics Data System (ADS)

Johnson, C. S.; Fan, Y.; Miller, K. G.; Browning, J. V.

2017-12-01

The tide gauge at Sandy Hook, NJ, a spit of sand extending 8 km into Raritan Bay of New York and New Jersey, recorded a 20th century rate of relative sea-level (RSL) rise of 4.0±0.5 mm/yr, significantly higher than the 1.4±0.2 mm/yr rate of global mean sea-level rise for the twentieth century. The Battery, NY tide gauge, located atop crystalline bedrock 26 km to the northwest, recorded a 20th century rate of RSL rise of 3.0±0.3 mm/yr and reflects the regional rate of RSL rise. After global and regional effects are removed from the rate of RSL rise at Sandy Hook, the remaining 0.9±0.5 mm/yr is due to local effects, autocompaction and groundwater extraction induced compaction, within the unconsolidated Cretaceous to recent sediments underlying Sandy Hook. Our previous work showed that 0.13 mm/yr (90% CI 0.01-0.34 mm/yr) is due to autocompaction of young (≤13,350 cal yrs BP), highly compressible, Quaternary sediments underlying Sandy Hook. The remaining 0.8 mm/yr (90% CI 0.3-1.2 mm/yr) is hypothesized to be due to regional and local groundwater extraction. Previous studies have shown that Sandy Hook is within the cone of depression for Monmouth County, NJ. Historical records indicate that pumping for Ft. Hancock, a military installation on Sandy Hook, depleted some shallow aquifers as early as 1905. Visual MODFLOW was used to model the northern portion of the New Jersey coastal plain. Well logs from the northern New Jersey coastal plain were compiled and interpreted to refine subsurface geometries and build the model grid. Well permits provided modern locations and rates of groundwater extraction. 1,794 observation wells were used to calibrate the model. We present model results simulating the change in regional and local groundwater flow and hydraulic pressures from pre-development ( 1880) to present. The pressure changes were then used to calculate the potential changes in thickness of each of the stratigraphic units underneath Sandy Hook between 1880 and

Upgrading food wastes by means of bromelain and papain to enhance growth and immunity of grass carp (Ctenopharyngodon idella).

PubMed

Choi, W M; Lam, C L; Mo, W Y; Wong, M H

2016-04-01

The fast growing of global aquaculture industry accompanied with increasing pressure on the supply and price of traditional feed materials (e.g., fish meal and soy bean meal). This circumstance has urged the need to search alternative sources of feed stuff. Food waste was used as feed stuff in rearing fish which possess substantial protein and lipid. Grass carp are major species reared in Hong Kong with lower nutritional requirements; it is also an ideal species for investigating the feasibility of using food waste as fish feeds for local aquaculture industry. The growth and immunity, reflected by total protein, total immunologlobulin (IgI), and nitroblue tetrazolium (NBT) activity of grass carp blood, were depressed when feeding with food waste feeds without enzymes. However, the supplementation of bromelain and papain in fish feed enhanced the efficient use of food waste by grass carp, which in turn improved the fish immunity. The present results indicated that the addition of those enzymes could enhance the feed utilization by fish and hematological parameters of grass carp, and the improvement on growth and immunity superior to the control (commercial feed) was observed with the addition of bromelain and papain supplement. Addition of 1 and 2 % mixture of bromelain and papain could significantly enhance the lipid utilization in grass carp.

The Hooked Galaxy

NASA Astrophysics Data System (ADS)

2006-06-01

Life is not easy, even for galaxies. Some indeed get so close to their neighbours that they get rather distorted. But such encounters between galaxies have another effect: they spawn new generations of stars, some of which explode. ESO's VLT has obtained a unique vista of a pair of entangled galaxies, in which a star exploded. Because of the importance of exploding stars, and particularly of supernovae of Type Ia [1], for cosmological studies (e.g. relating to claims of an accelerated cosmic expansion and the existence of a new, unknown, constituent of the universe - the so called 'Dark Energy'), they are a preferred target of study for astronomers. Thus, on several occasions, they pointed ESO's Very Large Telescope (VLT) towards a region of the sky that portrays a trio of amazing galaxies. MCG-01-39-003 (bottom right) is a peculiar spiral galaxy, with a telephone number name, that presents a hook at one side, most probably due to the interaction with its neighbour, the spiral galaxy NGC 5917 (upper right). In fact, further enhancement of the image reveals that matter is pulled off MCG-01-39-003 by NGC 5917. Both these galaxies are located at similar distances, about 87 million light-years away, towards the constellation of Libra (The Balance). ESO PR Photo 22/06 ESO PR Photo 22/06 The Hooked Galaxy and its Companion NGC 5917 (also known as Arp 254 and MCG-01-39-002) is about 750 times fainter than can be seen by the unaided eye and is about 40,000 light-years across. It was discovered in 1835 by William Herschel, who strangely enough, seems to have missed its hooked companion, only 2.5 times fainter. As seen at the bottom left of this exceptional VLT image, a still fainter and nameless, but intricately beautiful, barred spiral galaxy looks from a distance the entangled pair, while many 'island universes' perform a cosmic dance in the background. But this is not the reason why astronomers look at this region. Last year, a star exploded in the vicinity of the hook

The effect of Chaetoceros calcitrans extract on hematology common carp (Cyprinus carpio) infected by Aeromonas salmonicida

NASA Astrophysics Data System (ADS)

Maftuch; Wulan, N. D. A.; Suprastyani, H.; Wijayanto, E.; Noercholis, M.; Prihanto, A. A.; Kurniawan, A.

2018-04-01

The application of C. calcitrans extract in carp (C. carpio) is expected to inhibit the growth of A. salmonicida. A. salmonicida-infected common carp (C. carpio) were treated with the extract of C. calcitrans. Hematology, erythrocyte, leukocyte, hematocrit and hemoglobin test analysis was observed. The result indicated that the extract can be used to treat the infected fish. The best dose was treatment of D with 45.3 ppm.

Establishment of a new cell line from the snout tissue of golden pompano Trachinotus ovatus, and its application in virus susceptibility.

PubMed

Yu, Y; Wei, S; Wang, Z; Huang, X; Huang, Y; Cai, J; Li, C; Qin, Q

2016-06-01

A new marine-fish cell line, designated GPS, was established from the snout tissue of golden pompano Trachinotus ovatus. GPS cells multiplied well in Leibovitz's L-15 containing 10% foetal bovine serum (FBS) at 28° C and the cells have been subcultured for >60 passages. Polymerase chain reaction (PCR) amplification of 16S ribosomal (r)RNA confirmed the origin of this cell line from T. ovatus. Chromosome analysis showed that GPS cells exhibited chromosomal aneuploidy with a modal chromosome number of 54. Bright green fluorescence signal was observed in enhanced green fluorescent protein (EGFP)-N3 transfected cells, indicating that GPS cells could be used to investigate gene functions in vitro. The GPS cells were highly susceptible to Singapore grouper iridovirus (SGIV), which was demonstrated by the presence of severe cytopathic effect (CPE) and increased viral titres. Real-time quantitative PCR and Western blot analysis showed that the viral gene transcription and protein synthesis occurred during SGIV infection in GPS cells. Thus, this study described the characteristic of a new cell line from the snout tissue of T. ovatus that could be used as a tool for propagation of iridovirus and genetic manipulation to investigate host-pathogen interactions. © 2016 The Fisheries Society of the British Isles.

Improvement of common carp (Cyprinus carpio) sperm cryopreservation using a programable freezer.

PubMed

Bernáth, Gergely; Żarski, Daniel; Kása, Eszter; Staszny, Ádám; Várkonyi, Levente; Kollár, Tímea; Hegyi, Árpád; Bokor, Zoltán; Urbányi, Béla; Horváth, Ákos

2016-10-01

The applicability of a programmable freezer for the increased-scale cryopreservation of common carp sperm was investigated. The effect of different equilibration times, cryopreservation methods, extenders, dilution ratios, activating solutions on the post-thaw motility of common carp sperm was investigated. The suitable post-thaw storage time-interval as well as fertilizing capacity of cryopreserved sperm was also examined. The motility, curvilinear velocity (VCL) and straightness (STR) values did not decrease significantly during 60min of equilibration neither in equilibrated nor thawed groups. Motility parameters of thawed sperm were similar using a conventional cryopreservation technique using a polystyrene box [motility (33%), VCL (47μm/s) and STR (88%)] and a programmable freezer: [motility (32%), VCL (54μm/s) and STR (89%)]. The highest motility and VCL was measured with a sugar based extender (grayling extender) at a ratio 1:9 (motility: 52%, VCL: 76μm/s) and 1:20 (motility: 49%, VCL: 76μm/s). The activating solution for cyprinids (ASC) could prolong sperm movement up for 2min. A storage time of six hours following thawing did not have a significant effect on the motility parameters of thawed carp sperm. Agglutination was observed during cryopreservation of an elevated volume of sperm whereas motility 47%, VCL 62μm/s and STR 91% were measured after thawing. Fertilization rate with thawed sperm (32%) was significantly lower compared to the control group (73%). According to our results, the developed method using a programmable freezer is suitable for the cryopreservation of elevated number of straws. However, carp sperm agglutination during freezing may have a negative effect on the fertilizing capacity. Copyright © 2016 Elsevier Inc. All rights reserved.

Hook tool manufacture in New Caledonian crows: behavioural variation and the influence of raw materials.

PubMed

Klump, Barbara C; Sugasawa, Shoko; St Clair, James J H; Rutz, Christian

2015-11-18

New Caledonian crows use a range of foraging tools, and are the only non-human species known to craft hooks. Based on a small number of observations, their manufacture of hooked stick tools has previously been described as a complex, multi-stage process. Tool behaviour is shaped by genetic predispositions, individual and social learning, and/or ecological influences, but disentangling the relative contributions of these factors remains a major research challenge. The properties of raw materials are an obvious, but largely overlooked, source of variation in tool-manufacture behaviour. We conducted experiments with wild-caught New Caledonian crows, to assess variation in their hooked stick tool making, and to investigate how raw-material properties affect the manufacture process. In Experiment 1, we showed that New Caledonian crows' manufacture of hooked stick tools can be much more variable than previously thought (85 tools by 18 subjects), and can involve two newly-discovered behaviours: 'pulling' for detaching stems and bending of the tool shaft. Crows' tool manufactures varied significantly: in the number of different action types employed; in the time spent processing the hook and bending the tool shaft; and in the structure of processing sequences. In Experiment 2, we examined the interaction of crows with raw materials of different properties, using a novel paradigm that enabled us to determine subjects' rank-ordered preferences (42 tools by 7 subjects). Plant properties influenced: the order in which crows selected stems; whether a hooked tool was manufactured; the time required to release a basic tool; and, possibly, the release technique, the number of behavioural actions, and aspects of processing behaviour. Results from Experiment 2 suggested that at least part of the natural behavioural variation observed in Experiment 1 is due to the effect of raw-material properties. Our discovery of novel manufacture behaviours indicates a plausible scenario for the

[Case-control study on endobutton plate or clavicular hook plate for the repair of acromioclavicular joint dislocations].

PubMed

Zhu, Li; Yang, He-Jie; Zhao, Wan-Jun; Yang, Wu-Min; Zhou, Hui

2012-02-01

To compare the treatment results between Endobutton plate and clavicular hook plate for the treatment of acromioclavicular joint dislocations. From January 2009 to September 2010, 30 patients with acromioclavicular joint dislocations were treated with two different fixations: Endobutton plate (15 patients, including 12 males and 3 females, with a mean age of 38.5 +/- 8.2 years) and clavicular hook plate (15 patients, including 14 males and 1 female, with the mean age of 33.8 +/- 5.9 years). The mean operative time and blood loss were compared between the two groups. Joint function was assessed by the Karlsson standard. The mean operative time of the Endobutton group and the hook plate group were (61 +/- 8.6) min and (40 +/- 5.6) min. The average blood loss were (93 +/- 8.4) ml and (100 +/- 12.6) ml. There was no statistical difference between the two groups in the average blood loss and the mean operative time. According to the Karlsson standard, 13 patients got an excellent result, 2 good in the Endobutton group; and 7 patients got an excellent result and 7 good in hook plate group. The therapeutic effect in Endobutton group was better than that of hook plate group. In the treatment of acromioclavicular dislocation, fixation with Endobutton plate is believed to be better than hook plate fixation. It is an effective method for the repair of acromioclavicular joint dislocations in a short term. But its long term effects still need further follow up.

Extremely high myoglobin plasma concentrations producing hook effect in a critically ill patient.

PubMed

Kurt-Mangold, Michelle; Drees, Denny; Krasowski, Matthew D

2012-12-24

A 21-year old female suffered a cardiac arrest after a one week history of viral illness later shown to be caused by influenza B. The patient required extended cardiopulmonary resuscitation and had further complications including compartment syndrome. Plasma myoglobin concentration was measured using the Roche Diagnostics electrochemiluminescent myoglobin assay. The myoglobin concentration was 205,590 μg/l in an undiluted specimen, consistent with severe rhabdomyolysis. Subsequent myoglobin concentrations measured two days later showed dramatic decreases to approximately 1000 μg/l, raising suspicion of a hook effect. Dilution and re-analysis of the specimens revealed that the actual myoglobin concentrations were >395,000 μg/l, with one specimen possessing an estimated myoglobin concentration of >600,000 μg/l. Interestingly, three specimens from this patient did not show evidence of hook effect, with undiluted specimens producing myoglobin concentrations as high as 284,000 μg/l. Retrospective analysis of myoglobin results over an 8-year period did not reveal other cases with suspicion of hook effect. The case patient had the highest myoglobin concentrations out of 7301 specimens. This case illustrates that while the Roche myoglobin assay has a very wide dynamic range, hook effect can occur with extremely high concentrations of plasma myoglobin. Copyright © 2012 Elsevier B.V. All rights reserved.

Acute and Subacute toxicity study of Olaquindox by feeding to common carp (Cyprinus carpio L.).

PubMed

Yang, Qian; He, Jie; He, Sheng-Yu; Wang, Kai-Yu; Geng, Yi; Chen, De-Fang; Huang, Xiao-Li; Ou-Yang, Ping

2018-06-08

Olaquindox, is a growth-promoting feed additive for food-producing animals. As the banned medicinal feed additive, olaquindox in animal feed and water must be concerned as an important hazard index. To improve studies of the toxicity of olaquindox, we provide a toxicological effects of olaquindox on a common freshwater fish, Cyprinus carpio L. The results of acute toxicity tests showed that the 7d-LD 50 of olaquindox administered by feeding for common carp was determined to be 3746.3 mg/kg. We also found that the accumulation coefficient of olaquindox in carp was 1.45-1.9. Based on the studied hematological and blood biochemical parameters (RBCs count, hemoglobin content, ALT, AST and SOD activity), we found that olaquindox induced significant alterations in all studied parameters. Regarding bioaccumulation, the results showed that olaquindox had more efficiency to internalize fish tissues (liver, kidneys and muscle). The histopathological investigation of tissues from poisoning fish revealed various alterations that varied between adaptation responses and permanent tissue damage. Our results indicate that olaquindox are toxic to common carp and have obvious accumulation, and all the data from acute and subacute toxicity experiments in common carp may provide a useful tool for assessing the toxicity of olaquindox to aquatic organisms. Copyright © 2018 Elsevier Inc. All rights reserved.

Bioinformatics analysis of organizational and expressional characterizations of the IFNs, IRFs and CRFBs in grass carp Ctenopharyngodon idella.

PubMed

Liao, Zhiwei; Wan, Quanyuan; Su, Jianguo

2016-08-01

Interferons (IFNs) play crucial roles in the immune response of defense against viral infection and bacteria invasion. In the present study, we systematically identified and characterized the IFNs, their regulatory factors (Interferon Regulatory Factors, IRFs) and receptors (Cytokine Receptor Family B, CRFBs) in grass carp (Ctenopharyngodon idella). Grass carp IFNs can be classified into type I IFN (IFN-I) and type II IFN (IFN-II) like other teleosts. IFN-I consist of two groups with two (group I) or four (group II) cysteines in the mature peptide and can be further divided into three subgroups (IFN-a, -c and -d), containing four members: IFN1, IFN2, IFN3, IFN4 in grass carp. IFN-II contain two members, IFNγ2 with the similarity to mammalian IFNγ and a cyprinid specific IFNγ1 (IFNγ-rel) molecule. mRNA expression analyses of IFNs discovered that IFN1 and IFN-II were sustainably expressed in many tissues, while other IFN members were transiently expressed in specific tissues and time points. In the immune response, IFN transcriptions are primarily regulated through multiple IRFs after grass carp reovirus (GCRV) challenge. IRF family possess thirteen members in grass carp, which can be further divided into four subfamilies (IRF-1, -3, -4 and -5 subfamily), each of them plays different roles in the innate and adaptive immunity via various signaling pathways to interact with IFNs (mainly IFN-I). IFNs have to bind receptors (CRFBs) to perform their functions. CRFBs as IFN receptors contain six members in grass carp. The structure and expression characterizations of IFNs, IRFs and CRFBs were analyzed using bioinformatics tools. These results might provide basic data for the further functional research of IFN system, and deeply understand fish immune mechanisms against virus infection. Copyright © 2016 Elsevier Ltd. All rights reserved.

Alfaxalone as an intramuscular injectable anesthetic in koi carp (Cyprinus carpio).

PubMed

Bailey, Kate M; Minter, Larry J; Lewbart, Gregory A; Harms, Craig A; Griffith, Emily H; Posner, Lysa P

2014-12-01

Fish are commonly anesthetized with MS-222 (tricaine methanesulfonate), a sodium-channel-blocker used as an immersion anesthetic, but its mechanism of action as a general anesthetic is uncertain. Alfaxalone is a neurosteroid that acts at the GABA(A) receptors. Alfaxalone has been evaluated and was deemed successful as an immersion agent in koi carp. Alfaxalone is an effective intramuscular anesthetic in multiple species. A reliable intramuscular anesthetic in fish would be useful in multiple settings. The purpose of this study was to investigate alfaxalone as an intramuscular injectable anesthetic agent in koi carp (Cyprinus carpio). Eight koi carp were utilized in a crossover design. In each trial, six fish received 1 mg/kg, 5 mg/kg, or 10mg/kg of alfaxalone intramuscularly. They were assessed every 15 min for opercular rate and sedation score. The sedation score was based on a visual scale from 0 to 5, 0 indicating no response and 5 indicating absent righting reflex and anesthesia. Anesthetized koi were placed on a fish anesthesia delivery system (FADS). Time to anesthesia/recovery was recorded and heart rate was recorded every 15 min. Anesthesia was achieved in 0/6, 1/6, and 5/6 fish at 1, 5, and 10 mg/kg, respectively. Duration of anesthesia for one fish at 5 mg/kg was 2 hr. At 10 mg/kg, median anesthesia duration was 6.5 (3-10) hr. At 10 mg/kg, prolonged apnea (2-3 hr) was observed in 3/6 fish, 2/3 died under anesthesia, and 1/3 recovered 10 hr post-injection. Median peak sedation scores were 1.5, 2.5, and 5, at 1, 5, and 10 mg/kg, respectively. A dosage of 10 mg/kg alfaxalone resulted in 33% mortality. The duration of anesthesia and opercular rate were unpredictable. Due to variation in response despite consistent conditions, as well as risk of mortality, intramuscular alfaxalone cannot be recommended for anesthesia in koi carp.

Silymarin inhibits adipogenesis in the adipocytes in grass carp Ctenopharyngodon idellus in vitro and in vivo.

PubMed

Xiao, Peizhen; Yang, Zhou; Sun, Jian; Tian, Jingjing; Chang, Zhiguang; Li, Xuexian; Zhang, Baotong; Ye, Yuantu; Ji, Hong; Yu, Ermeng; Xie, Jun

2017-12-01

In this study, two experiments were performed to explore the function of silymarin in adipogenesis in grass carp (Ctenopharyngodon idellus) using in vitro and in vivo models. In experiment 1, differentiated grass carp pre-adipocytes were treated with silymarin for 6 days. Treatment with 100 μg mL -1 silymarin (SM100 group) significantly reduced triglyceride accumulation at day 6. The adipogenic gene expression levels of PPARγ, C/EBPα, SREBP1c, FAS, SCD1, and LPL, and the protein expression level of PPARγ were significantly down-regulated in the SM100 group. Additionally, the SM100 group had significantly lower reactive oxygen species production and reduced glutathione contents compared with the control in vitro. In experiment 2, the juvenile grass carp (mean body weight= 27.4 ± 0.17 g) were fed six isonitrogenous and isocaloric diets in a factorial design containing 0, 100, or 200 mg kg -1 silymarin (SM0, SM100, SM200) associated with either 4 or 8% lipid levels (low lipid, LL, and high lipid, HL, respectively) for 82 days. The results demonstrated that dietary silymarin supplementation significantly reduced the elevated intraperitoneal fat index in grass carp fed with high-lipid diets, and the gene expression of adipogenesis (PPARγ, FAS) when supplemented with dietary silymarin was notably lower than when no silymarin was supplemented under the high-lipid diets. Thus, our data suggest that silymarin suppressed lipid accumulation in grass carp both in vitro and in vivo, and the effect might be due to an influence on the expression of adipogenesis factors and ROS production partly associated with effects on antioxidant capability.

Genetic evidence for gonochoristic reproduction in gynogenetic silver crucian carp (Carassius auratus gibelio bloch) as revealed by RAPD assays.

PubMed

Zhou, L; Wang, Y; Gui, J F

2000-11-01

Sex evolution has been a debating focus in evolutionary genetics. In lower vertebrates of reptiles, amphibians, and fish, a species or a bioform reproduces either sexually or asexually but never both. A few species were found to consist of all females in fish. These all-female species can propagate by asexual reproduction modes, such as gynogenesis and hybridogenesis. However, the coexistence of sexuality and asexuality in a single species was recently noted only in a cyprinid fish silver crucian carp, Carassius auratus gibelio. This fish had been demonstrated to be capable of gynogenesis stimulated by sperm from other related species. Surprisingly, natural populations of this fish consist of a minor but significant portion (approx. 20%) of males. As different clones with specific phenotypic and genetic characteristics have been found, and RAPD markers specific to each clone have recently been identified, this fish offers many advantages for analyzing whether or not genetic recombination occurs between different clones. In this study, artificial propagation was performed in clone F and clone D. Ovulated eggs from clone F were divided into two parts and respectively inseminated with sperm from a clone D male and from a red common carp (Cyprinus carpio) male. The control clone D individuals were selected from gynogenetic offspring of clone D activated by sperm of red common carp. The phenotype and sex ratio in the experimental groups were also observed. Using RAPD molecular markers, which allow for reliable discrimination and genetic analysis of different clones, we have revealed direct molecular evidence for gonochoristic reproduction in the gynogenetic silver crucian carp and confirmed a previous hypothesis that the silver crucian carp might reproduce both gynogenetically and gonochoristically. Therefore, we conclude that the silver crucian carp possesses two reproductive modes, i.e., gynogenetic and gonochoristic reproduction. The response mechanism of two

Preliminary study on the relationship between dexamethasone and pathogen susceptibility on crucian carp (Carassius auratus).

PubMed

Qi, Xiao-Zhou; Li, Dong-Liang; Tu, Xiao; Song, Chen-Guang; Ling, Fei; Wang, Gao-Xue

2016-12-01

Dexamethasone, a known immunosuppressant, can inhibit the immune response and increase the amount of pathogen in body, but the role of dexamethasone affecting susceptibility of crucian carp (Carassius auratus) to pathogen is unclear. The effects of dexamethasone on susceptibility of crucian carp to Aeromonas hydrophila were investigated in this study. The fish were divided into four groups randomly and injected intraperitoneally by dexamethasone for 0 day (group D), 3 days (group C), 6 days (group B), and 9 days (group A), respectively. The serum lysozyme activity was significantly declined in group A, B and C. Relative immune gene expression such as il-1β, cxcl-8, tnfα and crp in kidney were down-regulation compared to group D. After that crucian carp were infected with A. hydrophila, crucian carp treated by dexamethasone had higher mortality (group A 95%, group B 76%, group C 31%) when compared to group D (4% mortality); the amount of pathogen in was significantly increased (P < 0.05) in liver, kidney and spleen of fish in group A-C compared to group D. These results implicated that higher susceptibility caused by dexamethasone may be induced by the decrease of lysozyme activity and the down-regulation of some immune genes. Copyright © 2016 Elsevier Ltd. All rights reserved.

In vitro polarization of carp leucocytes in response to the blood fluke Sanguinicola inermis Plehn, 1905 (Trematoda: Sanguinicolidae).

PubMed

Richards, D T; Hoole, D; Lewis, J W; Ewens, E; Arme, C

1996-05-01

An in vitro assay was used to determine the effects of Sanguinicola inermis adults and cercariae on the polarization responses of pronephric leucocytes of carp. Leucocytes were isolated and exposed to live adult flukes or cercariae for up to 48 h. Differences in polarization responses were related to the presence of the parasite, the presence or absence of carp serum and the time of incubation. The mean proportions of cells exhibiting polarization in unstimulated controls ranged from 5 to 30% over the experimental period. Within 15 min of exposure to adults or cercariae, significant increases in mean polarization responses were observed of up to 75% and levels remained higher than control values for over 24 h. Overall, the presence of normal carp serum, either untreated or heat inactivated, did not enhance the polarization responses of leucocytes incubated with only cercariae or adults. However, between 0.25 and 3 h, the presence of carp serum with cercariae significantly enhanced polarization responses when compared with cells incubated with cercariae alone.

Comparison of the intrusion effects on the maxillary incisors between implant anchorage and J-hook headgear.

PubMed

Deguchi, Toru; Murakami, Takashi; Kuroda, Shingo; Yabuuchi, Toshinori; Kamioka, Hiroshi; Takano-Yamamoto, Teruko

2008-05-01

Recently, miniscrews have been used to provide anchorage during orthodontic treatment, especially for incisor intrusion. Miniscrews during incisor intrusion are commonly used in implant orthodontics. Traditionally, effective incisor intrusion has been accomplished with J-hook headgear. In this study, we compared the effect of incisor intrusion, force vector, and amount of root resorption between implant orthodontics and J-hook headgear. Lateral cephalometric radiographs from 8 patients in the implant group and 10 patients in the J-hook headgear group were analyzed for incisor retraction. The estimated force vector was analyzed in the horizontal and vertical directions in both groups. Root resorption was also measured on periapical radiographs. In the implant group, significant reductions in overjet, overbite, maxillary incisor to palatal plane, and maxillary incisor to upper lip were observed after intrusion of the incisors. In the J-hook headgear group, significant reductions in overjet, overbite, maxillary incisor to upper lip, and maxillary incisor to SN plane were observed after intrusion of the incisors. There were significantly greater reductions in overbite, maxillary incisor to palatal plane, and maxillary incisor to upper lip in the implant group than in the J-hook headgear group. Estimated force analysis resulted in significantly more force in the vertical direction and less in the horizontal direction in the implant group. Furthermore, significantly less root resorption was observed in the implant group compared with the J-hook headgear group. The maxillary incisors were effectively intruded by using miniscrews as orthodontic anchorage without patient cooperation. The amount of root resorption was not affected by activating the ligature wire from the miniscrew during incisor intrusion.

In vivo analysis of acromioclavicular joint motion after hook plate fixation using three-dimensional computed tomography.

PubMed

Kim, Yoon Sang; Yoo, Yon-Sik; Jang, Seong Wook; Nair, Ayyappan Vijayachandran; Jin, Hyonki; Song, Hyun-Seok

2015-07-01

The clavicle hook plate can be used to treat acromioclavicular and coracoclavicular ligament injury or distal clavicular fracture with comminution. However, the hook plate can induce subacromial impingement, resulting in discomfort from the hardware. Our inclusion criteria were (1) men and women aged older than 20 years and (2) the presence of comminuted distal clavicular fractures (Neer type IIB) fixed with a hook plate (Synthes, Oberdorf, Switzerland). Three-dimensional computed tomography was obtained before removal of the hook plate. Seven patients were enrolled prospectively. The mean age was 42 years (range, 24-60 years). Zero degree images and abduction images were obtained. The sagittal cut surface was obtained 5 mm medial from the distal clavicle. The equator of the cut surface of the clavicle was compared with the full abduction model to analyze rotation. The center of the cut surface of the clavicle was compared with the full abduction model to analyze translation. The average difference in rotation of the distal clavicle between both shoulders was 16° (range, 3°-22°; P = .001). The mean difference in anterior translation of the distal clavicle was 2.2 mm (range, -0.7 to 5.6 mm; P = .030). Hook plate fixation at the acromioclavicular joint causes decreased internal rotation and increased anterior translation of the distal clavicle with respect to the medial acromion, indicating that the scapula relative to the thorax has decreased posterior tilting and increased external rotation in shoulders fixed using a hook plate. Copyright © 2015 Journal of Shoulder and Elbow Surgery Board of Trustees. Published by Elsevier Inc. All rights reserved.

Investigation of grass carp by-products from a fish farm in Vojvodina

NASA Astrophysics Data System (ADS)

Okanović, Đ.; Tasić, T.; Kormanjoš, Š.; Ikonić, P.; Šojić, B.; Pelić, M.; Ristić, M.

2017-09-01

The quantity of by-products obtained during grass carp primary processing and chemical characteristics of internal organs were investigated. The total average weight of byproducts was 783.69 g (36.99%) in relation to live body weight which was cca 2118.5 g. The by-product contributing the largest quantity to total live body weight was the head with 458.22 g (21.63% of live body weight), followed by complete internal organs and tail and fins, with weights of 198.03 g or 9.35% and 57.93 g or 2.73%, respectively. The chemical composition of internal organs from the grass carp was mostly water (65.55%), following by crude fats and crude proteins (17.47% and 13.35%, respectively). The low collagen content (13.43% of total crude protein) indicates the high nutritional quality of the protein content from internal organs. Nitrogenous complexes from the internal organs were predominantly proteins. Digestible nitrogen was approximately equal to total nitrogen (89.38%), indicating that all proteins of the internal organs had high biological value. Based on the results obtained, it can be concluded that carp internal organs could be important sources of proteins and fats, and thus, could be used in Serbia as a raw material for feed and technical fat production.

Quality and biochemical properties of artificially hibernated crucian carp for waterless preservation.

PubMed

Mi, Hongbo; Qian, Chunlu; Mao, Linchun

2012-12-01

The aim of this study was to explore the artificial hibernation of crucian carp for waterless preservation and to characterize the quality and biochemical properties during and after the hibernation. Anesthetized crucian carp using eugenol were stored at 8 °C with 90 % oxygen and 95-100 % relative humidity for 38 h and then transferred to fresh water to recover. Liquid loss and cooking loss had no significant changes (p > 0.05). The total volatile basic nitrogen content and 2-thiobarbituric acid value in hibernated fish were significantly higher (p < 0.05) than fresh and recovered groups. Serum cortisol, glutamic-oxaloacetic transaminase (GOT), alkaline phosphatase (AKP), and acid phosphatase (ACP) activities significantly increased (p < 0.05) during hibernation, while glutamic-pyruvic transaminase (GPT) had no significant change (p > 0.05). Both ACP and AKP activities decreased upon the fish recovered, but only the ACP activity returned to normal. However, there were increased serum glucose concentration, GOT and GPT activities in recovered fish. On the basis of these findings, it can be concluded that the artificially hibernated life of crucian carp was 38 h by the combination of anaesthetizing and low temperature. The muscle quality would not be influenced, and most of the stress responses would disappear after hibernated fish recovered.

Effects of Dietary Exposure to Zearalenone (ZEN) on Carp (Cyprinus carpio L.).

PubMed

Pietsch, Constanze; Kersten, Susanne; Valenta, Hana; Dänicke, Sven; Schulz, Carsten; Burkhardt-Holm, Patricia; Junge, Ranka

2015-08-26

The mycotoxin zearalenone (ZEN) is frequently contaminating animal feeds including feed used in aquaculture. In the present study, the effects of dietary exposure to ZEN on carp (Cyprinus carpio L.) were investigated. ZEN at three different concentrations (low dose: 332 µg kg(-1), medium dose: 621 µg kg(-1) and high dose: 797 µg kg(-1) final feed, respectively) was administered to juvenile carp for four weeks. Additional groups received the mycotoxin for the same time period but were fed with the uncontaminated diet for two more weeks to examine the reversibility of the ZEN effects. No effects on growth were observed during the feeding trial, but effects on haematological parameters occurred. In addition, an influence on white blood cell counts was noted whereby granulocytes and monocytes were affected in fish treated with the medium and high dose ZEN diet. In muscle samples, marginal ZEN and α-zearalenol (α-ZEL) concentrations were detected. Furthermore, the genotoxic potential of ZEN was confirmed by analysing formation of micronuclei in erythrocytes. In contrast to previous reports on other fish species, estrogenic effects measured as vitellogenin concentrations in serum samples were not increased by dietary exposure to ZEN. This is probably due to the fact that ZEN is rapidly metabolized in carp.

Effects of Dietary Exposure to Zearalenone (ZEN) on Carp (Cyprinus carpio L.)

PubMed Central

Pietsch, Constanze; Kersten, Susanne; Valenta, Hana; Dänicke, Sven; Schulz, Carsten; Burkhardt-Holm, Patricia; Junge, Ranka

2015-01-01

The mycotoxin zearalenone (ZEN) is frequently contaminating animal feeds including feed used in aquaculture. In the present study, the effects of dietary exposure to ZEN on carp (Cyprinus carpio L.) were investigated. ZEN at three different concentrations (low dose: 332 µg kg−1, medium dose: 621 µg kg−1 and high dose: 797 µg kg−1 final feed, respectively) was administered to juvenile carp for four weeks. Additional groups received the mycotoxin for the same time period but were fed with the uncontaminated diet for two more weeks to examine the reversibility of the ZEN effects. No effects on growth were observed during the feeding trial, but effects on haematological parameters occurred. In addition, an influence on white blood cell counts was noted whereby granulocytes and monocytes were affected in fish treated with the medium and high dose ZEN diet. In muscle samples, marginal ZEN and α-zearalenol (α-ZEL) concentrations were detected. Furthermore, the genotoxic potential of ZEN was confirmed by analysing formation of micronuclei in erythrocytes. In contrast to previous reports on other fish species, estrogenic effects measured as vitellogenin concentrations in serum samples were not increased by dietary exposure to ZEN. This is probably due to the fact that ZEN is rapidly metabolized in carp. PMID:26343724

Lowering Temperature is the Trigger for Glycogen Build-Up and Winter Fasting in Crucian Carp (Carassius carassius).

PubMed

Varis, Joonas; Haverinen, Jaakko; Vornanen, Matti

2016-02-01

Seasonal changes in physiology of vertebrate animals are triggered by environmental cues including temperature, day-length and oxygen availability. Crucian carp (Carassius carassius) tolerate prolonged anoxia in winter by using several physiological adaptations that are seasonally activated. This study examines which environmental cues are required to trigger physiological adjustments for winter dormancy in crucian carp. To this end, crucian carp were exposed to changing environmental factors under laboratory conditions: effects of declining water temperature, shortening day-length and reduced oxygen availability, separately and in different combinations, were examined on glycogen content and enzyme activities involved in feeding (alkaline phosphatase, AP) and glycogen metabolism (glycogen synthase, GyS; glycogen phosphorylase, GP). Lowering temperature induced a fall in activity of AP and a rise in glycogen content and rate of glycogen synthesis. Relative mass of the liver, and glycogen concentration of liver, muscle and brain increased with lowering temperature. Similarly activity of GyS in muscle and expression of GyS transcripts in brain were up-regulated by lowering temperature. Shortened day-length and oxygen availability had practically no effects on measured variables. We conclude that lowering temperature is the main trigger in preparation for winter anoxia in crucian carp.

Columbia Smelting & Refining Works Red Hook, Brooklyn, New York

EPA Pesticide Factsheets

The site is the former location of a secondary lead smelter called Columbia Smelting and Refining Works (Columbia), and the extent of lead-contaminated soil from the smelter, in the mixed-use neighborhood of Red Hook in Brooklyn, New York. The footprint of

Improved Methods for Capture, Extraction, and Quantitative Assay of Environmental DNA from Asian Bigheaded Carp (Hypophthalmichthys spp.)

PubMed Central

Turner, Cameron R.; Miller, Derryl J.; Coyne, Kathryn J.; Corush, Joel

2014-01-01

Indirect, non-invasive detection of rare aquatic macrofauna using aqueous environmental DNA (eDNA) is a relatively new approach to population and biodiversity monitoring. As such, the sensitivity of monitoring results to different methods of eDNA capture, extraction, and detection is being investigated in many ecosystems and species. One of the first and largest conservation programs with eDNA-based monitoring as a central instrument focuses on Asian bigheaded carp (Hypophthalmichthys spp.), an invasive fish spreading toward the Laurentian Great Lakes. However, the standard eDNA methods of this program have not advanced since their development in 2010. We developed new, quantitative, and more cost-effective methods and tested them against the standard protocols. In laboratory testing, our new quantitative PCR (qPCR) assay for bigheaded carp eDNA was one to two orders of magnitude more sensitive than the existing endpoint PCR assays. When applied to eDNA samples from an experimental pond containing bigheaded carp, the qPCR assay produced a detection probability of 94.8% compared to 4.2% for the endpoint PCR assays. Also, the eDNA capture and extraction method we adapted from aquatic microbiology yielded five times more bigheaded carp eDNA from the experimental pond than the standard method, at a per sample cost over forty times lower. Our new, more sensitive assay provides a quantitative tool for eDNA-based monitoring of bigheaded carp, and the higher-yielding eDNA capture and extraction method we describe can be used for eDNA-based monitoring of any aquatic species. PMID:25474207

Improved methods for capture, extraction, and quantitative assay of environmental DNA from Asian bigheaded carp (Hypophthalmichthys spp.).

PubMed

Turner, Cameron R; Miller, Derryl J; Coyne, Kathryn J; Corush, Joel

2014-01-01

Indirect, non-invasive detection of rare aquatic macrofauna using aqueous environmental DNA (eDNA) is a relatively new approach to population and biodiversity monitoring. As such, the sensitivity of monitoring results to different methods of eDNA capture, extraction, and detection is being investigated in many ecosystems and species. One of the first and largest conservation programs with eDNA-based monitoring as a central instrument focuses on Asian bigheaded carp (Hypophthalmichthys spp.), an invasive fish spreading toward the Laurentian Great Lakes. However, the standard eDNA methods of this program have not advanced since their development in 2010. We developed new, quantitative, and more cost-effective methods and tested them against the standard protocols. In laboratory testing, our new quantitative PCR (qPCR) assay for bigheaded carp eDNA was one to two orders of magnitude more sensitive than the existing endpoint PCR assays. When applied to eDNA samples from an experimental pond containing bigheaded carp, the qPCR assay produced a detection probability of 94.8% compared to 4.2% for the endpoint PCR assays. Also, the eDNA capture and extraction method we adapted from aquatic microbiology yielded five times more bigheaded carp eDNA from the experimental pond than the standard method, at a per sample cost over forty times lower. Our new, more sensitive assay provides a quantitative tool for eDNA-based monitoring of bigheaded carp, and the higher-yielding eDNA capture and extraction method we describe can be used for eDNA-based monitoring of any aquatic species.

Hook plate fixation for acute acromioclavicular dislocations without coracoclavicular ligament reconstruction: a functional outcome study in military personnel.

PubMed

Kumar, Narinder; Sharma, Vyom

2015-08-01

The aim of our study was to evaluate the shoulder function after clavicular hook plate fixation of acute acromioclavicular dislocations (Rockwood type III) in a population group consisting exclusively of high-demand military personnel. This prospective study was carried out at a tertiary care military orthopaedic centre during 2012-2013 using clavicular hook plate for management of acromioclavicular injuries without coracoclavicular ligament reconstruction in 33 patients. All patients underwent routine implant removal after 16 weeks. The functional outcome was assessed at 3, 6 and 12 months after hook plate removal and 2 years from the initial surgery using the Constant Murley and UCLA Scores. All the patients were male serving soldiers and had sustained acromioclavicular joint dislocation (Rockwood type III). Mean age of the patient group was 34.24 years (21-55 years). The mean follow-up period in this study was 23.5 months (20-26 months) after hook plate fixation and an average of 19.9 months (17-22 months) after hook plate removal. The average Constant Score at 3 months after hook plate removal was 60.3 as compared to 83.7 and 90.3 at 6 months and 1 year, respectively, and an average of 91.8 at the last follow-up that was approximately 2 years after initial surgery which was statistically significant (p value <0.05). The UCLA Score was an average of 15.27, 25.9 and 30.1 at 3, 6 months and 1 year, respectively, after removal of hook plate which improved further an average of 32.3 at the last follow-up, which was also statistically significant (p value <0.05). Clavicular hook plate fixation without coracoclavicular ligament reconstruction is a good option for acute acromioclavicular dislocations producing excellent medium-term functional results in high-demand soldiers.

Mitigating the Hook Effect in Lateral Flow Sandwich Immunoassays Using Real-Time Reaction Kinetics.

PubMed

Rey, Elizabeth G; O'Dell, Dakota; Mehta, Saurabh; Erickson, David

2017-05-02

The quantification of analyte concentrations using lateral flow assays is a low-cost and user-friendly alternative to traditional lab-based assays. However, sandwich-type immunoassays are often limited by the high-dose hook effect, which causes falsely low results when analytes are present at very high concentrations. In this paper, we present a reaction kinetics-based technique that solves this problem, significantly increasing the dynamic range of these devices. With the use of a traditional sandwich lateral flow immunoassay, a portable imaging device, and a mobile interface, we demonstrate the technique by quantifying C-reactive protein concentrations in human serum over a large portion of the physiological range. The technique could be applied to any hook effect-limited sandwich lateral flow assay and has a high level of accuracy even in the hook effect range.

Quantitative distributions of different cholinesterases and inhibition of acetylcholinesterase by metidathion and paraquat in alimentary canal of common carp.

PubMed

Láng, G; Kufcsák, O; Szegletes, T; Nemcsók, J

1997-07-01

1. The cholinesterases play an important role in the innervation of organs. The ratio of solubilized to membrane-bound cholinesterase and the quantitative distributions of acetylcholinesterase and butyrylcholinesterase were measured in different segments of the gut of carp (Cyprinus carpio) connected with different types of nerve-muscle synapses in different parts of the alimentary tract. 2. The inhibition of acetylcholinesterase (EC 3.1.1.7.) by the herbicide paraquat and the insecticide metidathion was measured in different parts of the gut of carp. 3. Metidathion and paraquat significantly decreased the activity of acetylcholinesterase in different segments of the alimentary tract of common carp, in a concentration-dependent manner.

Complementary DNA cloning and organ expression of cytochrome P450 1C2 in carp (Cyprinus carpio).

PubMed

Kaminishi, Yoshio; El-Kady, Mohamed A H; Mitsuo, Ryoichi; Itakura, Takao

2007-01-01

Cytochrome P450 (CYP) genes, which make up a large gene superfamily, are known to play an important role in drug metabolism. The CYP1 family, one of the gene families of the CYP superfamily, has three subfamilies of genes whose sequences have been deposited in the GenBank/EMBL thus far: CYP1A, CYP1B, and CYP1C. Mammals as well as fish confront numerous foreign chemicals in the environment that may accumulate to toxic levels unless they are metabolized and eliminated by processes largely mediated by CYP enzymes. A new complementary DNA of the CYP1C subfamily encoding CYP1C2 was isolated from the carp liver after a single intraperitoneal injection of beta-napthoflavone (BNF). The full-length cDNA obtained contained a 5' noncoding region of 198 bp, an open reading frame of 1575 bp coding for 524 amino acids and a stop codon, and a 3' noncoding region of 531 bp. The predicted molecular weight of the protein was approximately 59.3 kDa. The amino acid sequence deduced from the carp CYP1C2 sequence showed a similarity of 76.6% with that deduced from our previously reported carp CYP1C1. It exhibited similarities of 77.3, 73.7, and 76.4% with those deduced from scup CYP1C2, scup CYP1C1, and Japanese eel CYP1C1 sequences, respectively. Carp CYP1C2 cDNA showed similarities with reported CYP1Bs of teleosts and mammals, namely, 47.6, 45.3, 45.7, 44.0, and 44.6% for carp, plaice, human, rat, and mouse CYP1B1s, respectively, while it exhibited a similarity of 49.0% with carp CYP1B2. The carp CYP1C2 sequence was aligned with the CYP1 sequences and has been deposited in the GenBank/EMBL data bank with the accession number AY437777. The phylogenetic tree constructed using fish and mammalian CYP1 sequences suggested a closer relationship of CYP1C with CYP1B than with CYP1A. The tree showed possibile existence of CYP1C subfamily genes in mammalian species. Northern blot analysis of the liver, intestines, kidneys, and gills revealed a distinct induced expression only in the kidneys

Astronaut James Newman with latch hook for tether device

NASA Image and Video Library

1993-09-19

STS051-26-002 (12-22 Sept 1993) --- Astronaut James H. Newman, mission specialist, shows off a latch hook for a tether device used during the STS-51 extravehicular activity (EVA) on September 16, 1993. Newman, on Discovery's middeck, appears surrounded by sleep restraints.

COMPUTER-ASSISTED MOTION ANALYSIS OF SPERM FROM THE COMMON CARP

EPA Science Inventory

Computer-assisted semen analysis (CASA) technology was applied to the measurement of sperm motility parameters in the common carp Cyprinus carpio. Activated sperm were videotaped at 200 frames s-1 and analysed with the CellTrak/S CASA research system. The percentage of motile cel...

Effect of modified atmosphere and vacuum packaging on TVB-N production of rainbow trout (Oncorhynchus mykiss) and carp (Cyprinus carpio) cuts

NASA Astrophysics Data System (ADS)

Babić Milijašević, J.; Milijašević, M.; Đinović-Stojanović, J.; Vranić, D.

2017-09-01

The aim of our research was to examine the influence of packaging in modified atmosphere and vacuum on the total volatile basic nitrogen (TVB-N) content in muscle of rainbow trout (Oncorhynchus mykiss) and common carp (Cyprinus carpio), as well as to determine the most suitable gas mixtures for packing of these freshwater species. Three sample groups of trout and carp cuts were investigated. The two groups were packaged in modified atmosphere with different gas ratios: 90%CO2+10%N2 (MAP 1) and 60%CO2+40%N2 (MAP 2), whereas the third group of fish cuts were vacuum packaged. During trials, the trout and carp cuts were stored in refrigerator at 3°C±0.5°C. Determination of TVB-N was performed on 1, 4, 7, 9, 12 and 14 days of storage. The obtained results indicate that the investigated mixtures of gases and vacuum had a significant influence on the values of TVB-N in trout and carp cuts. The lowest increase in TVB-N was established in trout and carp cuts packaged in MAP 1, whereas the highest increase was established in vacuum packaged cuts. Based on the obtained results, it can be concluded that the gas mixture consisting of 90% CO2 and 10% N2 was the most suitable for packaging of fresh trout and carp cuts in terms of TVB-N value.

Azadirachtin, a neem-derived biopesticide, impairs behavioral and hematological parameters in carp (Cyprinus carpio).

PubMed

Murussi, Camila R; Menezes, Charlene C; Nunes, Mauro E M; Araújo, Maria do Carmo S; Quadros, Vanessa A; Rosemberg, Denis B; Loro, Vania L

2016-11-01

Azadirachtin (Aza) is a promisor biopesticide used in organic production and aquaculture. Although this compound is apparently safe, there is evidence that it may have deleterious effects on fish. Behavioral and hematological tests are grouped into a set of parameters that may predict potential toxicity of chemical compounds. Here, we investigate the effects of Aza, in the commercial formulation Neenmax ™ , on carp (Cyprinus carpio) by defining LC 50 (96 h), and testing behavioral and hematological parameters. In our study, LC 50 was estimated at 80 μL/L. We exposed carp to Aza at 20, 40, and 60 μL/L, values based on 25, 50, and 75% of LC 50 , respectively. At 60 μL/L, Aza promoted significant changes in several parameters, increasing the distance traveled and absolute turn angle. In addition, the same concentration decreased the time spent immobile and the number of immobile episodes. Hematological parameters, such as hematocrit, hemoglobin, hematimetrics index, and red cell distribution, were decreased at 60 μL/L Aza exposure. In conclusion, our study demonstrates that 60 μL/L Aza altered locomotor activity, motor pattern, and hematological parameters, suggesting potential toxicity to carp after acute exposure. In addition, this is the first report that evaluates the actions of a chemical contaminant using automated behavioral tracking of carp, which may be a useful tool for assessing the potential toxicity of biopesticides in conjunction with hematological tests. © 2015 Wiley Periodicals, Inc. Environ Toxicol 31: 1381-1388, 2016. © 2015 Wiley Periodicals, Inc.

Effect of dietary betaine on growth performance, antioxidant capacity and lipid metabolism in blunt snout bream fed a high-fat diet.

PubMed

Adjoumani, Jean-Jacques Yao; Wang, Kaizhou; Zhou, Man; Liu, Wenbin; Zhang, Dingdong

2017-12-01

An 8-week feeding experiment was conducted to determine the effect of dietary betaine levels on the growth performance, antioxidant capacity, and lipid metabolism in high-fat diet-fed blunt snout bream (Megalobrama amblycephala) with initial body weight 4.3 ± 0.1 g [mean ± SEM]. Five practical diets were formulated to contain normal-fat diet (NFD), high-fat diet (HFD), and high-fat diet with betaine addition (HFB) at difference levels (0.6, 1.2, 1.8%), respectively. The results showed that the highest final body weight (FBW), weight gain ratio (WGR), specific growth rate (SGR), condition factor (CF), and feed intake (FI) (P < 0.05) were obtained in fish fed 1.2% betaine supplementation, whereas feed conversion ratio (FCR) was significantly lower in the same group compared to others. Hepatosomatic index (HSI) and abdominal fat rate (AFR) were significantly high in fat group compared to the lowest in NDF and 1.2% betaine supplementation, while VSI and survival rate (SR) were not affected by dietary betaine supplementation. Significantly higher (P < 0.05), plasma total cholesterol (TC), triglycerides (TG), low-density lipoprotein (LDL), aspartate transaminase (AST), alanine transaminase (ALT), cortisol, and lower high-density lipoprotein (HDL) content were observed in HFD but were improved when supplemented with 1.2% betaine. In addition, increase in superoxide dismutase (SOD), catalase (CAT), and reduced glutathione (GSH) in 1.2% betaine inclusion could reverse the increasing malondialdehyde (MDA) level induced by HFD. Based on the second-order polynomial analysis, the optimum growth of blunt snout bream was observed in fish fed HFD supplemented with 1.2% betaine. HFD upregulated fatty acid synthase messenger RNA (mRNA) expression and downregulated carnitine palmitoyltransferase 1, peroxisome proliferator-activated receptor α, and microsomal triglyceride transfer protein mRNA expression; nevertheless, 1.2% betaine supplementation significantly reversed

Targeted disruption of sp7 and myostatin with CRISPR-Cas9 results in severe bone defects and more muscular cells in common carp

PubMed Central

Zhong, Zhaomin; Niu, Pengfei; Wang, Mingyong; Huang, Guodong; Xu, Shuhao; Sun, Yi; Xu, Xiaona; Hou, Yi; Sun, Xiaowen; Yan, Yilin; Wang, Han

2016-01-01

The common carp (Cyprinus carpio) as one of the most important aquaculture fishes produces over 3 million metric tones annually, approximately 10% the annual production of the all farmed freshwater fish worldwide. However, the tetraploidy genome and long generation-time of the common carp have made its breeding and genetic studies extremely difficult. Here, TALEN and CRISPR-Cas9, two versatile genome-editing tools, are employed to target common carp bone-related genes sp7, runx2, bmp2a, spp1, opg, and muscle suppressor gene mstn. TALEN were shown to induce mutations in the target coding sites of sp7, runx2, spp1 and mstn. With CRISPR-Cas9, the two common carp sp7 genes, sp7a and sp7b, were mutated individually, all resulting in severe bone defects; while mstnba mutated fish have grown significantly more muscle cells. We also employed CRISPR-Cas9 to generate double mutant fish of sp7a;mstnba with high efficiencies in a single step. These results demonstrate that both TALEN and CRISPR-Cas9 are highly efficient tools for modifying the common carp genome, and open avenues for facilitating common carp genetic studies and breeding. PMID:26976234

Meanest foundations and nobler superstructures: Hooke, Newton and the "compounding of the celestiall motions of the planets

NASA Astrophysics Data System (ADS)

Gal, Ofer

This book is a historical-epistemological study of one the most consequential idea of early modern celestial mechanics: Robert Hooke's proposal to "compoun[d] the celestial motions of the planets of a direct motion by the tangent & an attractive motion towards a central body," a proposal which Isaac Newton adopted and realized in his Principia. Hooke's Programme was revolutionary both cosmologically and mathematically. It presented "the celestial motions," the proverbial symbol of stability and immutability, as a process of continuous change, and prescribed only parameters of rectilinear motions and rectilinear attractions for calculating their closed curved orbits. Yet the traces of Hooke's construction of his Programme for the heavens lead through his investigations in such earthly disciplines as microscopy, practical optics and horology, and the mathematical tools developed by Newton to accomplish it appear no less local and goal-oriented than Hooke's lenses and springs. This transgression of the boundaries between the theoretical, experimental and technological realms is reminiscent of Hooke's own free excursions in and out of the circles occupied by gentlemen-philosophers, university mathematicians, instrument makers, technicians and servants. It presents an opportunity to examine the social and epistemological distinctions, relations and hierarchies between those realms and their inhabitants, and compels a critical assessment of the philosophical categories they embody.

School Safety in a Post-Sandy Hook World

ERIC Educational Resources Information Center

Trump, Kenneth S.

2014-01-01

In this report the author, who is a school safety expert, provides information about school safety in a post-Sandy Hook world. He presents the following: (1) Continuum of Threats and Responses; (2) The role social media plays; (3) Reliable Best Practices; (4) Policy and Funding--Climate and Context; (5) Policy and Funding--Things to Avoid; and (6)…

The "hook effect" causing a negative pregnancy test in a patient with an advanced molar pregnancy.

PubMed

Winder, Abigail D; Mora, Adria Suarez; Berry, Emily; Lurain, John R

2017-08-01

•At high hCG levels in molar pregnancies, a "hook effect" can cause an artificially negative value.•Delay in diagnosis of a molar pregnancy due to the "hook effect" can lead to severe complications.•Suspicion of a molar pregnancy should be communicated so a diluted sample is used to quantify hCG.

Incorporating basic and applied approaches to evaluate the effects of invasive Asian Carp on native fishes: A necessary first step for integrated pest management.

PubMed

Phelps, Quinton E; Tripp, Sara J; Bales, Kyle R; James, Daniel; Hrabik, Robert A; Herzog, David P

2017-01-01

Numerous studies throughout North America allege deleterious associations among invasive Asian Carp and native fishes; however, no empirical evidence on a system-wide scale exists. We used Mississippi River Basin fish community data collected by the Long Term Resource Monitoring program and the Missouri Department of Conservation to evaluate possible interaction between Asian Carp and native fishes. Results from two decades of long-term monitoring throughout much of the Mississippi River suggest that Silver Carp relative abundance has increased while relative abundance (Bigmouth Buffalo [F 3, 8240 = 6.44, P<0.01] and Gizzard Shad [F 3, 8240 = 31.04, P<0.01]) and condition (Bigmouth Buffalo [slope = -0.11; t = -1.71; P = 0.1014] and Gizzard Shad [slope = -0.39; t = -3.02; P = 0.0073]) of native planktivores have declined. Floodplain lake qualitative evaluations yielded similar results; floodplain lake fish communities were likely altered (i.e., reductions in native species) by Silver Carp. Furthermore, laboratory experiments corroborated field evidence; Silver Carp negatively influence native planktivores through competition for prey (all comparisons, P > 0.05). To this end, this study provides evidence that Silver Carp are likely adversely influencing native fishes; however, mere presence of Silver Carp in the system does not induce deleterious effects on native fishes. To the best of our knowledge, this evaluation is the first to describe the effects of Asian Carp throughout the Mississippi River Basin and could be used to reduce the effects of Asian Carp on native biota through an integrated pest management program as suggested by congressional policy. Despite the simplicity of the data analyzed and approach used, this study provides a framework for beginning to identify the interactions of invasive fish pests on native fishes (i.e., necessary first step of integrated pest management). However, knowledge gaps remain. We suggest future efforts should conduct more

Incorporating basic and applied approaches to evaluate the effects of invasive Asian Carp on native fishes: A necessary first step for integrated pest management

PubMed Central

Tripp, Sara J.; James, Daniel; Hrabik, Robert A.; Herzog, David P.

2017-01-01

Numerous studies throughout North America allege deleterious associations among invasive Asian Carp and native fishes; however, no empirical evidence on a system-wide scale exists. We used Mississippi River Basin fish community data collected by the Long Term Resource Monitoring program and the Missouri Department of Conservation to evaluate possible interaction between Asian Carp and native fishes. Results from two decades of long-term monitoring throughout much of the Mississippi River suggest that Silver Carp relative abundance has increased while relative abundance (Bigmouth Buffalo [F 3, 8240 = 6.44, P<0.01] and Gizzard Shad [F 3, 8240 = 31.04, P<0.01]) and condition (Bigmouth Buffalo [slope = -0.11; t = -1.71; P = 0.1014] and Gizzard Shad [slope = -0.39; t = -3.02; P = 0.0073]) of native planktivores have declined. Floodplain lake qualitative evaluations yielded similar results; floodplain lake fish communities were likely altered (i.e., reductions in native species) by Silver Carp. Furthermore, laboratory experiments corroborated field evidence; Silver Carp negatively influence native planktivores through competition for prey (all comparisons, P > 0.05). To this end, this study provides evidence that Silver Carp are likely adversely influencing native fishes; however, mere presence of Silver Carp in the system does not induce deleterious effects on native fishes. To the best of our knowledge, this evaluation is the first to describe the effects of Asian Carp throughout the Mississippi River Basin and could be used to reduce the effects of Asian Carp on native biota through an integrated pest management program as suggested by congressional policy. Despite the simplicity of the data analyzed and approach used, this study provides a framework for beginning to identify the interactions of invasive fish pests on native fishes (i.e., necessary first step of integrated pest management). However, knowledge gaps remain. We suggest future efforts should conduct more

A Novel Soluble Immune-Type Receptor (SITR) in Teleost Fish: Carp SITR Is Involved in the Nitric Oxide-Mediated Response to a Protozoan Parasite

PubMed Central

Ribeiro, Carla M. S.; Bird, Steve; Raes, Geert; Ghassabeh, Gholamreza H.; Schijns, Virgil E. J. C.; Pontes, Maria J. S. L.; Savelkoul, Huub F. J.; Wiegertjes, Geert F.

2011-01-01

Background The innate immune system relies upon a wide range of germ-line encoded receptors including a large number of immunoglobulin superfamily (IgSF) receptors. Different Ig-like immune receptor families have been reported in mammals, birds, amphibians and fish. Most innate immune receptors of the IgSF are type I transmembrane proteins containing one or more extracellular Ig-like domains and their regulation of effector functions is mediated intracellularly by distinct stimulatory or inhibitory pathways. Methodology/Principal Findings Carp SITR was found in a substracted cDNA repertoire from carp macrophages, enriched for genes up-regulated in response to the protozoan parasite Trypanoplasma borreli. Carp SITR is a type I protein with two extracellular Ig domains in a unique organisation of a N-proximal V/C2 (or I-) type and a C-proximal V-type Ig domain, devoid of a transmembrane domain or any intracytoplasmic signalling motif. The carp SITR C-proximal V-type Ig domain, in particular, has a close sequence similarity and conserved structural characteristics to the mammalian CD300 molecules. By generating an anti-SITR antibody we could show that SITR protein expression was restricted to cells of the myeloid lineage. Carp SITR is abundantly expressed in macrophages and is secreted upon in vitro stimulation with the protozoan parasite T. borreli. Secretion of SITR protein during in vivo T. borreli infection suggests a role for this IgSF receptor in the host response to this protozoan parasite. Overexpression of carp SITR in mouse macrophages and knock-down of SITR protein expression in carp macrophages, using morpholino antisense technology, provided evidence for the involvement of carp SITR in the parasite-induced NO production. Conclusion/Significance We report the structural and functional characterization of a novel soluble immune-type receptor (SITR) in a teleost fish and propose a role for carp SITR in the NO-mediated response to a protozoan parasite. PMID

Characterization of the microbiota in lightly salted bighead carp (Aristichthys nobilis) fillets stored at 4 °C.

PubMed

Liu, Xiaochang; Zhang, Yuemei; Li, Dapeng; Luo, Yongkang

2017-04-01

The microbiota of unsalted and salted (dry-cured with 2% salt) bighead carp (Aristichthys nobilis) fillets during storage at 4 °C were identified by 16S rRNA gene analysis. Eleven genera were present in the initial microbiota of bighead carp fillets, where Acinetobacter, Aeromonas and Kocuria were the dominant bacteria. As storage time progressed, the microbial composition of both unsalted and salted fillets became less diverse. Additionally, differences in microbiota were observed between these two treatments. For unsalted bighead carp fillets, Aeromonas became the dominant genus at the end of storage and Pseudomonas was found less commonly. For salted fillets, Pseudomonas was the only bacteria identified at the end of storage. Copyright © 2016 Elsevier Ltd. All rights reserved.

Susceptibility of koi x crucian carp and koi x goldfish hybrids to koi herpesvirus (KHV) and the development of KHV disease (KHVD).

PubMed

Bergmann, S M; Sadowski, J; Kiełpiński, M; Bartłomiejczyk, M; Fichtner, D; Riebe, R; Lenk, M; Kempter, J

2010-03-01

Hybrids of koi, Cyprinus carpio x crucian carp, Carassius carassius and koi x goldfish, Carassius auratus, proved to be susceptible to koi herpesvirus (KHV, syn. CyHV-3) and developed KHV disease (KHVD). While hybrids of koi x goldfish were partly resistant to mortality following infection by immersion, most koi x crucian carp hybrids died after bath infection. KHV DNA was detected in dead fish but also in all surviving animals by different polymerase chain reactions (PCRs). According to these results, hybrid crossbreeding does not seem to prevent severe losses associated with KHV in terms of inducing KHVD. The present study showed severe losses after a waterborne KHV infection of between 35% and 100% in koi x goldfish and koi x crucian carp hybrids as well as in SPF carp.

The discovery of microorganisms by Robert Hooke and Antoni Van Leeuwenhoek, fellows of the Royal Society.

PubMed

Gest, Howard

2004-05-01

The existence of microscopic organisms was discovered during the period 1665-83 by two Fellows of The Royal Society, Robert Hooke and Antoni van Leeuwenhoek. In Micrographia (1665), Hooke presented the first published depiction of a microganism, the microfungus Mucor. Later, Leeuwenhoek observed and described microscopic protozoa and bacteria. These important revelations were made possible by the ingenuity of Hooke and Leeuwenhoek in fabricating and using simple microscopes that magnified objects from about 25-fold to 250-fold. After a lapse of more than 150 years, microscopy became the backbone of our understanding of the roles of microbes in the causation of infectious diseases and the recycling of chemical elements in the biosphere.

Rose Prickles and Asparagus Spines--Different Hook Structures as Attachment Devices in Climbing Plants.

PubMed

Gallenmüller, Friederike; Feus, Amélie; Fiedler, Kathrin; Speck, Thomas

2015-01-01

Functional morphology and biomechanical properties of hook structures functioning as attachment devices in the leaning climbers Rosa arvensis, Rosa arvensis 'Splendens', Asparagus falcatus and Asparagus setaceus are analysed in order to investigate the variability in closely related species as well as convergent developments of hook structure and properties in distant systematic lineages (monocots and dicots). Prickles and spines were characterised by their size, orientation and the maximum force measured at failure in mechanical tests performed with traction forces applied at different angles. In Rosa arvensis and Rosa arvensis 'Splendens' three types of prickles differing largely in geometrical and mechanical properties are identified (prickles of the wild species and two types of prickles in the cultivar). In prickles of Rosa arvensis no particular orientation of the prickle tip is found whereas in the cultivar Rosa arvensis 'Splendens' prickles gradually gain a downward-orientation due to differential growth in the first weeks of their development. Differences in mechanical properties and modes of failure are correlated to geometrical parameters. In Asparagus falcatus and Asparagus setaceus spines are composed of leaf tissue, stem tissue and tissue of the axillary bud. Between species spines differ in size, orientation, distribution along the stem, tissue contributions and mechanical properties. The prickles of Rosa arvensis and its cultivar and the spines of the studied Asparagus species have several traits in common: (1) a gradual change of cell size and cell wall thickness, with larger cells in the centre and smaller thick-walled cells at the periphery of the hooks, (2) occurrence of a diversity of shape and geometry within one individual, (3) failure of single hooks when submitted to moderate mechanical stresses (Fmax/basal area < 35 N/mm²) and (4) failure of the hooks without severe stem damage (at least in the tested wild species).

Short-term effects of genistein on gamete quality, steroidogenesis and histological changes in gonads in gibel carp, Carassius auratus gibelio

USDA-ARS?s Scientific Manuscript database

The objective of the present investigation was to determine effects of genistein or 17ß-estradiol (E2) on reproductive physiology in gibel carp, Carassius auratus gibelio, during prespawning phase. Maturing gibel carp of both sexes received intraperitoneal injections of E2 (10 µg/g body weight), one...

First evidence of bighead carp wild recruitment in Western Europe, and its relation to hydrology and temperature.

PubMed

Milardi, Marco; Chapman, Duane; Lanzoni, Mattia; Long, James M; Castaldelli, Giuseppe

2017-01-01

Bighead carp (Hypophthalmichthys nobilis) have been introduced throughout Europe, mostly unintentionally, and little attention has been given to their potential for natural reproduction. We investigated the presence of young-of-the-year bighead carp in an irrigation canal network of Northern Italy and the environmental conditions associated with spawning in 2011-2015. The adult bighead carp population of the canal network was composed by large, likely mature, individuals with an average density of 45.2 kg/ha (over 10 fold more than in the main river). The 29 juvenile bighead carp found were 7.4-13.1 cm long (TL) and weighed 9.5-12.7 g. Using otolith-derived spawning dates we estimated that these juveniles were 94-100 days old, placing their fertilization and hatch dates in mid-to-end-June. Using this information in combination with thermal and hydraulic data, we examined the validity of existing models predicting the onset of spawning conditions and the viability of egg pathways to elucidate spawning location of the species. While evidence of reproduction was not found every year, we determined that potentially viable spawning conditions (annual degree-days and temperature thresholds) and pathways of egg drift suitable for hatching are present in short, slow-flowing canals.

First evidence of bighead carp wild recruitment in Western Europe, and its relation to hydrology and temperature

USGS Publications Warehouse

Milardi, Marco; Chapman, Duane C.; Long, James M.; Castaldelli, Giuseppe

2017-01-01

Bighead carp (Hypophthalmichthys nobilis) have been introduced throughout Europe, mostly unintentionally, and little attention has been given to their potential for natural reproduction. We investigated the presence of young-of-the-year bighead carp in an irrigation canal network of Northern Italy and the environmental conditions associated with spawning in 2011–2015. The adult bighead carp population of the canal network was composed by large, likely mature, individuals with an average density of 45.2 kg/ha (over 10 fold more than in the main river). The 29 juvenile bighead carp found were 7.4–13.1 cm long (TL) and weighed 9.5–12.7 g. Using otolith-derived spawning dates we estimated that these juveniles were 94–100 days old, placing their fertilization and hatch dates in mid-to-end-June. Using this information in combination with thermal and hydraulic data, we examined the validity of existing models predicting the onset of spawning conditions and the viability of egg pathways to elucidate spawning location of the species. While evidence of reproduction was not found every year, we determined that potentially viable spawning conditions (annual degree-days and temperature thresholds) and pathways of egg drift suitable for hatching are present in short, slow-flowing canals.

Gut content microbiota of introduced bigheaded carps (Hypophthalmichthys spp.) inhabiting the largest shallow lake in Central Europe.

PubMed

Borsodi, Andrea K; Szabó, Attila; Krett, Gergely; Felföldi, Tamás; Specziár, András; Boros, Gergely

2017-01-01

Studying the microbiota in the alimentary tract of bigheaded carps (Hypophthalmichthys spp.) gained special interest recently, as these types of investigations on non-native fish species may lead to a better understanding of their ecological role and feeding habits in an invaded habitat. For microbiological examinations, bigheaded carp gut contents and water column samples from Lake Balaton (Hungary) were collected from spring to autumn in 2013. Denaturing Gradient Gel Electrophoresis (DGGE) and pyrosequencing of the 16S rRNA gene were performed to reveal the composition. According to the DGGE patterns, bacterial communities of water samples separated clearly from that of the intestines. Moreover, the bacterial communities in the foreguts and hindguts were also strikingly dissimilar. Based on pyrosequencing, both foregut and hindgut samples were predominated by the fermentative genus Cetobacterium (Fusobacteria). The presence of some phytoplankton taxa and the high relative abundance of cellulose-degrading bacteria in the guts suggest that intestinal microbes may have an important role in digesting algae and making them utilizable for bigheaded carps that lack cellulase enzyme. In turn, the complete absence of typical heterotrophic freshwater bacteria in all studied sections of the intestines indicated that bacterioplankton probably has a negligible role in the nutrition of bigheaded carps. Copyright © 2016 Elsevier GmbH. All rights reserved.

Effect of water hardness and dissolved-solid concentration on hatching success and egg size in bighead carp

USGS Publications Warehouse

Chapman, Duane C.; Deters, Joseph E.

2009-01-01

Bighead carp Hypophthalmichthys nobilis is an Asian species that has been introduced to the United States and is regarded as a highly undesirable invader. Soft water has been said to cause the bursting of Asian carp eggs and thus has been suggested as a factor that would limit the spread of this species. To evaluate this, we subjected fertilized eggs of bighead carp to waters with a wide range of hardness and dissolved-solid concentrations. Hatching rate and egg size were not significantly affected by the different water qualities. These results, combined with the low hardness (28–84 mg/L) of the Yangtze River (the primary natal habitat of Hypophthalmichthys spp.), suggest that managers and those performing risk assessments for the establishment of Hypophthalmichthys spp. should be cautious about treating low hardness and dissolved-solid concentrations as limiting factors.

Molecular and functional characterization of the scavenger receptor CD36 in zebrafish and common carp.

PubMed

Fink, Inge R; Benard, Erica L; Hermsen, Trudi; Meijer, Annemarie H; Forlenza, Maria; Wiegertjes, Geert F

2015-02-01

CD36 is a scavenger receptor which has been studied closely in mammals where it is expressed by many different cell types and plays a role in highly diverse processes, both homeostatic and pathologic. It is among other things important in the innate immune system, in angiogenesis, and in clearance of apoptotic cells, and it is also involved in lipid metabolism and atherosclerosis. Recently, in the cephalochordate amphioxus a primitive CD36 family member was described, which was present before the divergence of CD36 from other scavenger receptor B family members, SCARB1 and SCARB2. Not much is known on the Cd36 molecule in teleost fish. We therefore studied Cd36 in both zebrafish and common carp, two closely related cyprinid fish species. Whereas a single cd36 gene is present in zebrafish, carp has two cd36 genes, and all show conserved synteny compared to mammalian CD36. The gene expression of carp cd36 is high in brain, ovary and testis but absent in immune organs. Although in mammals CD36 expression in erythrocytes, monocytes and macrophages is high, gene expression studies in leukocyte subtypes of adult carp and zebrafish larvae, including thrombocytes and macrophages provided no indication for any substantial expression of cd36 in immune cell types. Surprisingly, analysis of the cd36 promoter region does show the presence of several binding sites for transcription factors known to regulate immune responses. Overexpression of carp cd36 locates the receptor on the cell surface of mammalian cell lines consistent with the predicted topology of cyprinid Cd36 with a large extracellular domain, two transmembrane domains, and short cytoplasmic tails at both ends. Gene expression of cd36 is down-regulated during infection of zebrafish with Mycobacterium marinum, whereas knockdown of cd36 in zebrafish larvae led to higher bacterial burden upon such infection. We discuss the putative role for Cd36 in immune responses of fish in the context of other members of the

Occurrence of Bothriocephalus acheilognathi (Cestoda, Bothriocephallidea) in grass carp Ctenopharyngodon idella in the Changjiang River drainage

NASA Astrophysics Data System (ADS)

Xi, Bingwen; Wang, Guitang; Xie, Jun

2011-05-01

Bothriocephalus acheilognathi is a potentially serious pathogen in wild or cultured fish in worldwide distribution. We examined 58-farmed grass carp from Nanchang in the Changjiang (Yangtze) River drainage, from which 20.7% were found to harbor the parasite with an infection intensity of 36.9±54.7. The parasites were identified based on morphology and rDNA ITS sequence analysis. The present report represents the first record of the parasite in grass carp Ctenopharyngodon idella in the river drainage.

Complete genome sequence and architecture of crucian carp Carassius auratus herpesvirus (CaHV).

PubMed

Zeng, Xiao-Tao; Chen, Zhong-Yuan; Deng, Yuan-Sheng; Gui, Jian-Fang; Zhang, Qi-Ya

2016-12-01

Crucian carp Carassius auratus herpesvirus (CaHV) was isolated from diseased crucian carp with acute gill hemorrhages and high mortality. The CaHV genome was sequenced and analyzed. The data showed that it consists of 275,348 bp and contains 150 predicted ORFs. The architecture of the CaHV genome differs from those of four cyprinid herpesviruses (CyHV1, CyHV2, SY-C1, CyHV3), with insertions, deletions and the absence of a terminal direct repeat. Phylogenetic analysis of the DNA polymerase sequences of 17 strains of Herpesvirales members, and the concatenated 12 core ORFs from 10 strains of alloherpesviruses showed that CaHV clustered together with members of the genus Cyprinivirus, family Alloherpesviridae.

33 CFR 334.102 - Sandy Hook Bay, Naval Weapons Station EARLE, Piers and Terminal Channel, restricted area...

Code of Federal Regulations, 2012 CFR

2012-07-01

... 33 Navigation and Navigable Waters 3 2012-07-01 2012-07-01 false Sandy Hook Bay, Naval Weapons... DANGER ZONE AND RESTRICTED AREA REGULATIONS § 334.102 Sandy Hook Bay, Naval Weapons Station EARLE, Piers..., shall be enforced by the Commanding Officer, Naval Weapons Station Earle, and/or other persons or...

33 CFR 334.102 - Sandy Hook Bay, Naval Weapons Station EARLE, Piers and Terminal Channel, restricted area...

Code of Federal Regulations, 2014 CFR

2014-07-01

... 33 Navigation and Navigable Waters 3 2014-07-01 2014-07-01 false Sandy Hook Bay, Naval Weapons... DANGER ZONE AND RESTRICTED AREA REGULATIONS § 334.102 Sandy Hook Bay, Naval Weapons Station EARLE, Piers..., shall be enforced by the Commanding Officer, Naval Weapons Station Earle, and/or other persons or...

33 CFR 334.102 - Sandy Hook Bay, Naval Weapons Station EARLE, Piers and Terminal Channel, restricted area...

Code of Federal Regulations, 2010 CFR

2010-07-01

... 33 Navigation and Navigable Waters 3 2010-07-01 2010-07-01 false Sandy Hook Bay, Naval Weapons... DANGER ZONE AND RESTRICTED AREA REGULATIONS § 334.102 Sandy Hook Bay, Naval Weapons Station EARLE, Piers..., shall be enforced by the Commanding Officer, Naval Weapons Station Earle, and/or other persons or...

33 CFR 334.102 - Sandy Hook Bay, Naval Weapons Station EARLE, Piers and Terminal Channel, restricted area...

Code of Federal Regulations, 2011 CFR

2011-07-01

... 33 Navigation and Navigable Waters 3 2011-07-01 2011-07-01 false Sandy Hook Bay, Naval Weapons... DANGER ZONE AND RESTRICTED AREA REGULATIONS § 334.102 Sandy Hook Bay, Naval Weapons Station EARLE, Piers..., shall be enforced by the Commanding Officer, Naval Weapons Station Earle, and/or other persons or...

33 CFR 334.102 - Sandy Hook Bay, Naval Weapons Station EARLE, Piers and Terminal Channel, restricted area...

Code of Federal Regulations, 2013 CFR

2013-07-01

... 33 Navigation and Navigable Waters 3 2013-07-01 2013-07-01 false Sandy Hook Bay, Naval Weapons... DANGER ZONE AND RESTRICTED AREA REGULATIONS § 334.102 Sandy Hook Bay, Naval Weapons Station EARLE, Piers..., shall be enforced by the Commanding Officer, Naval Weapons Station Earle, and/or other persons or...

Trypsin inhibitory activity and gel-enhancing effect of sarcoplasmic proteins from common carp.

PubMed

Siriangkanakun, Siriphon; Yongsawatdigul, Jirawat

2012-10-01

Proteinase inhibitory activity of sarcoplasmic protein (SP) extracted from common carp (Cyprinus carpio) muscle and its gel-improving ability were investigated. SPs displayed 89% and 54% inhibitory activity toward trypsin at 40 and 65 °C, respectively. Protein bands with molecular mass of 69, 50, 44, 41, and 35 kDa appeared on trypsin inhibitory activity staining under nonreducing condition when incubated at 40 °C, while 2 protein bands at 54 and 35 kDa were observed at 65 °C. Addition of SP at 0.18 g protein/100 g increased textural properties of threadfin bream surimi gel. However, when SP was added in combination with various CaCl(2) concentrations (0.1% to 0.5%) it did not further improve textural properties as compared to the addition of SP alone. Retention of myosin heavy chain of threadfin bream surimi was greater with the addition of SP. These results indicated that the gel-enhancing effect of common carp SP was due to the inhibitory activity toward endogenous trypsin-like proteinases in threadfin bream surimi. Sarcoplasmic protein from common carp muscle could be used as a functional protein ingredient that minimizes muscle proteolysis and improves textural properties of surimi containing trypsin-like endogenous proteinases. © 2012 Institute of Food Technologists®

Identification of Aroma-active Compounds in Essential Oil from Uncaria Hook by Gas Chromatography- Mass Spectrometry and Gas Chromatography-Olfactometry.

PubMed

Iwasa, Megumi; Nakaya, Satoshi; Maki, Yusuke; Marumoto, Shinsuke; Usami, Atsushi; Miyazawa, Mitsuo

2015-01-01

The chemical composition of essential oil extracted from Uncaria Hook ("Chotoko" in Japanese), the branch with curved hook of the herbal medicine Uncaria rhynchophylla has been investigated by GC and GC-MS analyses. Eighty-four compounds, representing 90.8% of the total content was identified in oil obtained from Uncaria Hook. The main components i were (E)-cinnamaldehyde (13.4%), α-copaene (8.0%), methyl eugenol (6.8%), δ-cadinene (5.3%), and curcumene (3.6%). The important key aroma-active compounds in the oil were detected by gas chromatography-olfactometry (GC-O) and aroma extract dilution analysis (AEDA), using the flavor dilution (FD) factor to express the odor potency of each compounds. Furthermore, the odor activity value (OAV) has been used as a measure of the relative contribution of each compound to the aroma of the Uncaria Hook oil. The GC-O and AEDA results showed that α-copaene (FD = 4, OAV = 4376), (E)-linalool oxide (FD = 64, OAV = 9.1), and methyl eugenol (FD = 64, OAV = 29) contributed to the woody and spicy odor of Uncaria Hook oil, whereas furfural (FD = 8, OAV = 4808) contributed to its sweet odor. These results warrant further investigations of the application of essential oil from Uncaria Hook in the phytochemical and medicinal fields.

Gateway National Recreation Area - Sandy Hook Unit : parking management study

DOT National Transportation Integrated Search

2003-12-01

This report describes the Parking Management System (PMS) concept for the Sandy Hook Traveler Information System (TIS). The PMS is an important component of the TIS because it ultimately will be used to determine the open / closed status of the park....

Structural and genetic analysis of a mutant of Rhodobacter sphaeroides WS8 deficient in hook length control.

PubMed Central

González-Pedrajo, B; Ballado, T; Campos, A; Sockett, R E; Camarena, L; Dreyfus, G

1997-01-01

Motility in the photosynthetic bacterium Rhodobacter sphaeroides is achieved by the unidirectional rotation of a single subpolar flagellum. In this study, transposon mutagenesis was used to obtain nonmotile flagellar mutants from this bacterium. We report here the isolation and characterization of a mutant that shows a polyhook phenotype. Morphological characterization of the mutant was done by electron microscopy. Polyhooks were obtained by shearing and were used to purify the hook protein monomer (FlgE). The apparent molecular mass of the hook protein was 50 kDa. N-terminal amino acid sequencing and comparisons with the hook proteins of other flagellated bacteria indicated that the Rhodobacter hook protein has consensus sequences common to axial flagellar components. A 25-kb fragment from an R. sphaeroides WS8 cosmid library restored wild-type flagellation and motility to the mutant. Using DNA adjacent to the inserted transposon as a probe, we identified a 4.6-kb SalI restriction fragment that contained the gene responsible for the polyhook phenotype. Nucleotide sequence analysis of this region revealed an open reading frame with a deduced amino acid sequence that was 23.4% identical to that of FliK of Salmonella typhimurium, the polypeptide responsible for hook length control in that enteric bacterium. The relevance of a gene homologous to fliK in the uniflagellated bacterium R. sphaeroides is discussed. PMID:9352903

Accumulation of Mercury in The Tissues of the Great Cormorant (Phalacrocorax carbo) From Common Carp.

PubMed

Kral, Tomas; Blahova, Jana; Doubkova, Veronika; Farkova, Dagmar; Vecerek, Vladimir; Svobodova, Zdenka

2017-02-01

The aim of this work is to assess mercury content in the great cormorant in the Třeboň region pond systems (Czech Republic) in terms of its potential to accumulate mercury from common carp. Selected tissues samples were taken from 51 cormorants and 30 common carp. In the food chain the cormorant was found to have the potential to accumulate mercury, where the muscle total mercury was roughly 35 times higher compared to the total mercury content in the carp muscle as its food. A statistically significantly higher overall mercury content (p < 0.01) has been found in the kidney and liver (2.23 ± 0.30, 2.12 ± 0.22 mg/kg) compared to other tissues examined in cormorants. The proportion of muscle methylmercury in the total mercury content of the cormorant was within the range 64.3%-87.3%. The results can help us to gain a better understanding of how mercury is distributed and accumulated in the aquatic food chain.

Responses of invasive silver and bighead carp to a carbon dioxide barrier in outdoor ponds

USGS Publications Warehouse

Cupp, Aaron R.; Erickson, Richard A.; Fredricks, Kim T.; Swyers, Nicholas M.; Hatton, Tyson; Amberg, Jon J.

2017-01-01

Resource managers need for effective methods to prevent the movement of silver (Hypophthalmichthys molitrix) and bighead carp (H. nobilis) from the Mississippi River basin into the Laurentian Great Lakes. In this study, we evaluated dissolved carbon dioxide (CO2) as a barrier and deterrent to silver (278 ± 30.5 mm) and bighead (212 ± 7.7 mm) carp movement in continuous-flow outdoor ponds. As a barrier, CO2 significantly reduced upstream movement but was not 100% effective at blocking fish passage. As a deterrent, we observed a significant shift away from areas of high CO2 relative to normal movement before and after injection. Carbon dioxide concentrations varied across the pond during injection and reached maximum concentrations of 74.5±1.9 mg/L CO2; 29 532 – 41 393 µatm at the site of injection during three independent trials. We conclude that CO2 altered silver and bighead carp movement in outdoor ponds and recommend further research to determine barrier effectiveness during field applications.

Immunostimulatory effects of natural human interferon-alpha (huIFN-alpha) on carps Cyprinus carpio L.

PubMed

Watanuki, Hironobu; Chakraborty, Gunimala; Korenaga, Hiroki; Kono, Tomoya; Shivappa, R B; Sakai, Masahiro

2009-10-15

Human interferon-alpha (huIFN-alpha) is an important immunomodulatory substance used in the treatment and prevention of numerous infectious and immune-related diseases in animals. However, the immunostimulatory effects of huIFN-alpha in fish remain to be investigated. In the current study, the immune responses of the carp species Cyprinus carpio L. to treatment with huIFN-alpha were analyzed via measurement of superoxide anion production, phagocytic activity and the expression of cytokine genes including interleukin-1beta, tumor necrosis factor-alpha and interleukin 10. Low doses of huIFN-alpha were administered orally once a day for 3 days, and sampling was carried out at 1, 3 and 5 days post-treatment. Our results indicate that a low dose of huIFN-alpha significantly increased phagocytic activity and superoxide anion production in the carp kidney. The huIFN-alpha-treated fish also displayed a significant upregulation in cytokine gene expression. The current study demonstrates the stimulatory effects of huIFN-alpha on the carp immune system and highlights the immunomodulatory role of huIFN-alpha in fish.

Comparison of intestinal bacterial communities in grass carp, Ctenopharyngodon idellus, from two different habitats

NASA Astrophysics Data System (ADS)

Ni, Jiajia; Yu, Yuhe; Zhang, Tanglin; Gao, Lei

2012-09-01

The intestinal bacteria of vertebrates form a close relationship with their host. External and internal conditions of the host, including its habitat, affect the intestinal bacterial community. Similarly, the intestinal bacterial community can, in turn, influence the host, particularly with respect to disease resistance. We compared the intestinal bacterial communities of grass carp that were collected from farm-ponds or a lake. We conducted denaturing gradient gel electrophoresis of amplified 16S rRNA genes, from which 66 different operational taxonomic units were identified. Using both the unweighted pair-group method with arithmetic means clustering and principal component analysis ordination, we found that the intestinal bacterial communities from the two groups of pond fish were clustered together and inset into the clusters of wild fish, except for DF-7, and there was no significant correlation between genetic diversity of grass carp and their intestinal bacterial communities (Mantel one-tailed test, R=0.157, P=0.175). Cetobacterium appeared more frequently in the intestine of grass carp collected from pond. A more thorough understanding of the role played by intestinal microbiota on fish health would be of considerable benefit to the aquaculture industry.

Cyprinid herpesvirus 2 infection emerged in cultured gibel carp, Carassius auratus gibelio in China.

PubMed

Xu, Jin; Zeng, Lingbing; Zhang, Hui; Zhou, Yong; Ma, Jie; Fan, Yuding

2013-09-27

An epizootic with severe mortality has emerged in cultured gibel carp, Carassius auratus gibelio, in China since 2009, and caused huge economic loss. The signs and epidemiology background of the disease were investigated. Parasite examination, bacteria and virus isolation were carried out for pathogen isolation. The causative pathogen was obtained and identified as Cyprinid herpesvirus 2 (CyHV-2) by experimental infection, electron microscopy, cell culture, PCR assay and sequence alignment, designated as CyHV-2-JSSY. Experimental infection proved the high virulence of CyHV-2-JSSY to healthy gibel carp. Electron microscopy revealed that the viral nucleocapsid was hexagonal in shape measuring 110-120 nm in diameter with a 170-200 nm envelope. The virus caused significant CPE in Koi-Fin cells at the early passages, but not beyond the fifth passages. Sequence alignment of the partial viral helicase gene (JX566884) showed that it shared 99-100% identity to the published sequences of other CyHV-2 isolates. This study represented the first isolation and identification of CyHV-2 in cultured gibel carp in China and laid a foundation for the further studies of the disease. Copyright © 2013 Elsevier B.V. All rights reserved.

Transfissural Route Used for Preoperative Localization of Small Pulmonary Lesions with a Short Hook Wire and Suture System

DOE Office of Scientific and Technical Information (OSTI.GOV)

Iguchi, Toshihiro, E-mail: iguchi@ba2.so-net.ne.jp; Hiraki, Takao, E-mail: takaoh@tc4.so-net.ne.jp; Gobara, Hideo, E-mail: gobara@cc.okayama-u.ac.jp

2015-02-15

PurposeWe retrospectively evaluated the results of the transfissural route for preoperative localization with a short hook wire and suture system for video-assisted thoracoscopic surgery (VATS).MethodsEleven patients with 11 tumors underwent CT-guided transfissural placement of a hook wire before VATS. This route was selected for all patients, because the distance between the tumor and interlobar fissure was much shorter than the required distance traversed using the conventional approach. Complications were evaluated using the National Cancer Institute Common Terminology Criteria for Adverse Events Version 4.0.ResultsThe hook wire was successfully placed using the transfissural route in all but one case. Of these tenmore » successful placements, two tumors needed a second puncture for optimal placement, because the CT scan showed that the first hook wire was not properly placed in the lung. In one patient, we did not attempt replacement after the first placement was incorrect. In ten successful procedures, the mean distance traversed in the parenchyma of the unaffected lung lobe was 27.9 mm. The distance between the pleura and placed hook wire was significantly shorter than the estimated distance between the pleura and hook wire using the conventional route (mean 16.3 vs. 40.9 mm; P = 0.0002). Grade 1 adverse events occurred (11 pneumothoraxes and 4 pulmonary hemorrhages). No grade 2 or higher adverse event was observed.ConclusionsThe transfissural route used for preoperative localization before VATS is useful for selected patients because this route may allow for more limited lung parenchyma resection.« less

Magnetic Alignment in Carps: Evidence from the Czech Christmas Fish Market

PubMed Central

Hart, Vlastimil; Kušta, Tomáš; Němec, Pavel; Bláhová, Veronika; Ježek, Miloš; Nováková, Petra; Begall, Sabine; Červený, Jaroslav; Hanzal, Vladimír; Malkemper, Erich Pascal; Štípek, Kamil; Vole, Christiane; Burda, Hynek

2012-01-01

While magnetoreception in birds has been studied intensively, the literature on magnetoreception in bony fish, and particularly in non-migratory fish, is quite scarce. We examined alignment of common carps (Cyprinus carpio) at traditional Christmas sale in the Czech Republic. The sample comprised measurements of the directional bearings in 14,537 individual fish, distributed among 80 large circular plastic tubs, at 25 localities in the Czech Republic, during 817 sampling sessions, on seven subsequent days in December 2011. We found that carps displayed a statistically highly significant spontaneous preference to align their bodies along the North-South axis. In the absence of any other common orientation cues which could explain this directional preference, we attribute the alignment of the fish to the geomagnetic field lines. It is apparent that the display of magnetic alignment is a simple experimental paradigm of great heuristic potential. PMID:23227241

Quantification of eDNA shedding rates from invasive bighead carp Hypophthalmichthys nobilis and silver carp Hypophthalmichthys molitrix

USGS Publications Warehouse

Klymus, Katy E.; Richter, Catherine A.; Chapman, Duane C.; Paukert, Craig P.

2015-01-01

Wildlife managers can more easily mitigate the effects of invasive species if action takes place before a population becomes established. Such early detection requires sensitive survey tools that can detect low numbers of individuals. Due to their high sensitivity, environmental DNA (eDNA) surveys hold promise as an early detection method for aquatic invasive species. Quantification of eDNA amounts may also provide data on species abundance and timing of an organism’s presence, allowing managers to successfully combat the spread of ecologically damaging species. To better understand the link between eDNA and an organism’s presence, it is crucial to know how eDNA is shed into the environment. Our study used quantitative PCR (qPCR) and controlled laboratory experiments to measure the amount of eDNA that two species of invasive bigheaded carps (Hypophthalmichthys nobilis and Hypophthalmichthys molitrix) shed into the water. We first measured how much eDNA a single fish sheds and the variability of these measurements. Then, in a series of manipulative lab experiments, we studied how temperature, biomass (grams of fish), and diet affect the shedding rate of eDNA by these fish. We found that eDNA amounts exhibit a positive relationship with fish biomass, and that feeding could increase the amount of eDNA shed by ten-fold, whereas water temperature did not have an effect. Our results demonstrate that quantification of eDNA may be useful for predicting carp density, as well as densities of other rare or invasive species.

A long-snouted, multihorned tyrannosaurid from the Late Cretaceous of Mongolia

PubMed Central

Brusatte, Stephen L.; Carr, Thomas D.; Erickson, Gregory M.; Bever, Gabe S.; Norell, Mark A.

2009-01-01

Tyrannosaurid theropods are characterized by a generalized body plan, and all well-known taxa possess deep and robust skulls that are optimized for exerting powerful bite forces. The fragmentary Late Cretaceous Alioramus appears to deviate from this trend, but its holotype and only known specimen is incomplete and poorly described. A remarkable new tyrannosaurid specimen from the Maastrichtian (Late Cretaceous) of Mongolia, including a nearly complete and well-preserved skull and an extensive postcranium, represents a new species of Alioramus, Alioramus altai. This specimen conclusively demonstrates that Alioramus is a small, gracile, long-snouted carnivore that deviates from other tyrannosaurids in its body plan and presumably its ecological habits. As such, it increases the range of morphological diversity in one of the most familiar extinct clades. Phylogenetic analysis places Alioramus deep within the megapredatory Tyrannosauridae, and within the tyrannosaurine subclade that also includes Tarbosaurus and Tyrannosaurus. Both pneumatization and ornamentation are extreme compared with other tyrannosaurids, and the skull contains eight discrete horns. The new specimen is histologically aged at nine years old but is smaller than other tyrannosaurids of similar age. Despite its divergent cranial form, Alioramus is characterized by a similar sequence of ontogenetic changes as the megapredatory Tyrannosaurus and Albertosaurus, indicating that ontogenetic change is conservative in tyrannosaurids. PMID:19805035

Improvement for identification of heterophile antibody interference and AFP hook effect in immunoassays with multiplex suspension bead array system.

PubMed

Wang, Yajie; Yu, Jinsheng; Ren, Yuan; Liu, Li; Li, Haowen; Guo, Anchen; Shi, Congning; Fang, Fang; Juehne, Twyla; Yao, Jianer; Yang, Enhuan; Zhou, Xuelei; Kang, Xixiong

2013-11-15

A variety of immunoassays including multiplex suspension bead array have been developed for tumor marker detections; however, these assays could be compromised in their sensitivity and specificity by well-known heterophile antibody interference and hook effect. Using Luminex® multiplex suspension bead arrays, we modified protocols with two newly-developed solutions that can identify heterophile antibody interference and AFP hook effect. Effectiveness of the two solutions was assessed in serum samples from patients. Concentrations of 9 tumor markers in heterophile antibody positive samples assayed with Solution A, containing murine monoclonal antibodies and mouse serum, were significantly reduced when compared with those false high signals assayed without Solution A (all p<0.01). With incorporation of Solution H (fluorescent beads linked with AFP antigen), a new strategy for identification of AFP hook effect was established, and with this strategy AFP hook effect was identified effectively in serum samples with very high levels of AFP. Two proprietary solutions improve the identification of heterophile antibody interference and AFP hook effect. With these solutions, multiplex suspension bead arrays provide more reliable testing results in tumor marker detection where complex clinical serum samples are used. © 2013.