Science.gov

Sample records for horns rev offshore

  1. Modeling turbine wakes and power losses within the Horns Rev offshore wind farm using large-eddy simulation

    NASA Astrophysics Data System (ADS)

    Wu, Yu-Ting; Porte-Agel, Fernando

    2013-04-01

    A recently-developed large-eddy simulations (LES) framework is implemented to predict multiple wake flows and the associated power losses within the Horns Rev offshore wind farm under near-neutral stability conditions. A tuning-free Lagrangian scale-dependent dynamic subgrid-scale (SGS) model is used for the parametrization of the SGS stresses. The turbine-generated power outputs and the turbine-induced forces (e.g., thrust, lift, drag) are parameterized using two models: (a) the traditional actuator-disk model without rotation (ADM-NR), which uses the 1D momentum theory to relate the power output and the thrust force with a representative velocity over the rotor (e.g., the disk-averaged velocity); and (b) the actuator-disk model with rotation (ADM-R), which adopts blade element theory to calculate the lift and drag forces (that produce thrust, rotor shaft torque, and power) based on the local blade and flow characteristics. In general, the predicted power outputs obtained using the ADM-R are in good agreement with observed power data from the Horns Rev wind farm. The ADM-NR tends to underestimate the power output. A similar under-prediction is obtained using industry-standard wind-farm models such as the Wind Atlas Analysis and Application Program (WAsP). Simulations using different inflow conditions show that the mean wind direction has a strong effect on the spatial distributions of the time-averaged velocity and the turbulence intensity within the farm. These, in turn, affect the power output and the fatigue loads on the turbines. When the prevailing wind direction is parallel to the turbine rows (i.e., a full wake condition), the velocity deficit and the power losses are largest, and the turbulence intensity levels are highest and have a symmetric pattern (dual-peak at hub height) on both sides of the turbine wakes. A detailed analysis of the turbulence kinetic energy budget in the full wake condition shows an important effect of the increased turbulence level

  2. Mapping Wind Farm Loads and Power Production - A Case Study on Horns Rev 1

    NASA Astrophysics Data System (ADS)

    Galinos, Christos; Dimitrov, Nikolay; Larsen, Torben J.; Natarajan, Anand; Hansen, Kurt S.

    2016-09-01

    This paper describes the development of a wind turbine (WT) component lifetime fatigue load variation map within an offshore wind farm. A case study on the offshore wind farm Horns Rev I is conducted with this purpose, by quantifying wake effects using the Dynamic Wake Meandering (DWM) method, which has previously been validated based on CFD, Lidar and full scale load measurements. Fully coupled aeroelastic load simulations using turbulent wind conditions are conducted for all wind directions and mean wind speeds between cut-in and cut-out using site specific turbulence level measurements. Based on the mean wind speed and direction distribution, the representative 20-year lifetime fatigue loads are calculated. It is found that the heaviest loaded WT is not the same when looking at blade root, tower top or tower base components. The blade loads are mainly dominated by the wake situations above rated wind speed and the highest loaded blades are in the easternmost row as the dominating wind direction is from West. Regarding the tower components, the highest loaded WTs are also located towards the eastern central location. The turbines with highest power production are, not surprisingly, the ones facing a free sector towards west and south. The power production results of few turbines are compared with SCADA data. The results of this paper are expected to have significance for operation and maintenance planning, where the schedules for inspection and service activities can be adjusted to the requirements arising from the varying fatigue levels. Furthermore, the results can be used in the context of remaining fatigue lifetime assessment and planning of decommissioning.

  3. Modelling one row of Horns Rev wind farm with the Actuator Line Model with coarse resolution

    NASA Astrophysics Data System (ADS)

    Draper, M.; Guggeri, A.; Usera, G.

    2016-09-01

    Actuator models have been used to represent the presence of wind turbines in a simulation in the past few years. The Actuator Line Model (ALM) has shown to reproduce with reasonable accuracy the wind flow through wind turbines under different operational conditions. Nevertheless, there are not many simulations of wind farms performed with the ALM mainly because of its computational cost. The aim of the present paper is to evaluate the ALM in spatial resolutions coarser than what is generally recommended, also using larger time steps, in a simulation of a real wind farm. To accomplish this, simulations of one row of Horns Rev wind farm are performed, for different wind directions. It is concluded that the ALM is able to capture the main features of the interaction between wind turbines relaxing its resolution requirements. A sensitivity analysis is performed to assess the influence of the smearing factor and the spatial resolution.

  4. Effect of nacelle on the wake meandering in Horns Rev wind farm

    NASA Astrophysics Data System (ADS)

    Yang, Xiaolei; Foti, Daniel; Sotiropoulos, Fotis

    2016-11-01

    Turbine wake meandering has considerable effects on the velocity deficit and turbulence intensity in the wake. However, the mechanism for wake meandering is still not well understood and low-order models cannot take into account the wake meandering effects accurately. A recent work by Kang, Yang and Sotiropoulos showed that the nacelle has a significant effect on the wake meandering of a hydrokinetic turbine. To examine the nacelle contributions to wake meandering and wake interactions in utility-scale wind farms, we simulate the atmospheric turbulent flow over the Horns Rev wind farm using large-eddy simulation with actuator type models. In a preliminary simulation on a coarse grid using actuator line model for turbine blades without a nacelle model, the computed power shows overall good agreement with field measurements. Fine grid simulations using an actuator surface model for turbine blades with and without a nacelle model are being carried out. The corresponding results will be presented with analysis on wake meandering dynamics using the technique proposed by Horward et al. and Foti et al.. This work was supported by DOE (DE-AC04-94AL85000), Xcel Energy (Grant RD4-13) and Sandia National Laboratories. Computational resources were provided by National Renewable Energy Laboratory and University of Minnesota Supercomputing Institute.

  5. The Long distance wake behind Horns Rev I studied using large eddy simulations and a wind turbine parameterization in WRF

    NASA Astrophysics Data System (ADS)

    Eriksson, O.; Baltscheffsky, M.; Breton, S.-P.; Söderberg, S.; Ivanell, S.

    2017-05-01

    The aim of the present paper is to obtain a better understanding of long distance wakes generated by wind farms as a first step towards a better understanding of farm to farm interaction. The Horns Rev I (HR) wind farm is considered for this purpose, where comparisons are performed between microscale Large Eddy Simulations (LES) using an Actuator Disc model (ACD), mesoscale simulations in the Weather Research and Forecasting Model (WRF) using a wind turbine parameterization, production data as well as wind measurements in the wind farm wake. The LES is manually set up according to the wind conditions obtained from the mesoscale simulation as a first step towards a meso/microscale coupling. The LES using an ACD are performed in the EllipSys3D code. A forced boundary layer (FBL) approach is used to introduce the desired wind shear and the atmospheric turbulence field from the Mann model. The WRF uses a wind turbine parameterization based on momentum sink. To make comparisons with the LESs and the site data possible an idealized setup of WRF is used in this study. The case studied here considers a westerly wind direction sector (at hub height) of 270 ± 2.5 degrees and a wind speed of 8 ± 0.5 m/s. For both the simulations and the site data a neutral atmosphere is considered. The simulation results for the relative production as well as the wind speed 2 km and 6 km downstream from the wind farm are compared to site data. Further comparisons between LES and WRF are also performed regarding the wake recovery and expansion. The results are also compared to an earlier study of HR using LES as well as an earlier comparison of LES and WRF. Overall the results in this study show a better agreement between LES and WRF as well as better agreement between simulations and site data. The procedure of using the profile from WRF as inlet to LES can be seen as a simplified coupling of the models that could be developed further to combine the methods for cases of farm to farm

  6. Session: Offshore wind

    SciTech Connect

    Gaarde, Jette; Ram, Bonnie

    2004-09-01

    This session at the Wind Energy and Birds/Bats workshop consisted of two presentations. Due to time constraints, a discussion period was not possible. The session addressed the current state of offshore wind energy development. The first presentation ''Monitoring Program and Results: Horns Rev and Nysted'' by Jette Gaarde summarized selected environmental studies conducted to date at operating offshore wind turbine projects in Denmark and lessons from other offshore wind developments in Europe. Wildlife impacts studies from the Danish sites focused on birds, fish, and mammals. The second presentation ''What has the U.S. Wind Industry Learned from the European Example'' by Bonnie Ram provided an update on current permit applications for offshore wind developments in the U.S. as well as lessons that may be drawn from the European experience.

  7. Quantifying offshore wind resources from satellite wind maps: study area the North Sea

    NASA Astrophysics Data System (ADS)

    Hasager, C. B.; Barthelmie, R. J.; Christiansen, M. B.; Nielsen, M.; Pryor, S. C.

    2006-01-01

    Offshore wind resources are quantified from satellite synthetic aperture radar (SAR) and satellite scatterometer observations at local and regional scale respectively at the Horns Rev site in Denmark. The method for wind resource estimation from satellite observations interfaces with the wind atlas analysis and application program (WAsP). An estimate of the wind resource at the new project site at Horns Rev is given based on satellite SAR observations. The comparison of offshore satellite scatterometer winds, global model data and in situ data shows good agreement. Furthermore, the wake effect of the Horns Rev wind farm is quantified from satellite SAR images and compared with state-of-the-art wake model results with good agreement. It is a unique method using satellite observations to quantify the spatial extent of the wake behind large offshore wind farms. Copyright

  8. Geophysical data from offshore of the Gulf Islands National Seashore, Cat Island to Western Horn Island, Mississippi

    USGS Publications Warehouse

    Pendleton, E.A.; Baldwin, W.E.; Danforth, W.W.; DeWitt, N.T.; Forde, A.S.; Foster, D.S.; Kelso, K.W.; Pfeiffer, W.R.; Turecek, A.M.; Flocks, J.G.; Twichell, D.C.

    2011-01-01

    This report contains the geophysical and geospatial data that were collected along the western offshore side of the Gulf Islands of Mississippi on the research vessel Tommy Munro during two cruises in 2010. Geophysical data were collected by the U.S. Geological Survey in Woods Hole, Massachusetts, and St. Petersburg, Forida, in cooperation with the U.S. Army Corps of Engineers Mobile District. Bathymetric-sonar, sidescan-sonar, and Chirp seismic-reflection data were acquired with the following equipment, respectively: Systems Engineering and Assessment, Ltd., SwathPlus interferometric sonars; Klein 3000 and 3900 dual-frequency sidescan sonars; and an EdgeTech 512i Chirp sub-bottom profiling system. The long-term goals of this mapping effort are to produce high-quality, high-resolution geologic maps and interpretations that can be utilized to identify sand resources within the region, to better understand the Holocene evolution, and to anticipate future changes in this coastal system. Processed geospatial data files and the geophysical data provided in this report help attain these goals.

  9. Forecasting offshore wind speeds above the North Sea

    NASA Astrophysics Data System (ADS)

    Tambke, Jens; Lange, Matthias; Focken, Ulrich; Wolff, Jörg-Olaf; Bye, John A. T.

    2005-01-01

    We investigate the expected performance of short-term wind power prediction systems for offshore sites in the German Bight and at Horns Rev. Despite the special meteorological situation over the North Sea, it is found that the accuracy of wind speed predictions provided by the numerical prediction model of the German weather service is comparable to that of onshore predictions. However, although relative forecast errors look promising, the absolute errors are fairly large, with a root mean square error up to 3 m s-1 for the 48 h forecast. Moreover, vertical wind profiles which are typically needed to calculate the wind speed at hub height are considered at Horns Rev. In all thermal conditions the measured profiles show significant deviations from the expected shapes. The reason for this has to be clarified. Assuming that the deviations are due to the physical processes in the marine boundary layer, we present an alternative approach to derive wind profiles over the ocean which involves the inertial coupling of the Ekman layers of atmosphere and sea via a wave boundary layer with constant shear stress. Profiles calculated by this method are compared with measured profiles, showing rather good agreement. Copyright

  10. Offshore wind resource estimation from satellite SAR wind field maps

    NASA Astrophysics Data System (ADS)

    Hasager, C. B.; Nielsen, M.; Astrup, P.; Barthelmie, R.; Dellwik, E.; Jensen, N. O.; Jørgensen, B. H.; Pryor, S. C.; Rathmann, O.; Furevik, B. R.

    2005-10-01

    A wind resource estimation study based on a series of 62 satellite wind field maps is presented. The maps were retrieved from imaging synthetic aperture radar (SAR) data. The wind field maps were used as input to the software RWT, which calculates the offshore wind resource based on spatial averaging (footprint modelling) of the wind statistic in each satellite image. The calculated statistics can then be input to the program WAsP and used in lieu of in-situ observations by meteorological instruments. A regional wind climate map based on satellite SAR images delineates significant spatial wind speed variations. The site of investigation was Horns Rev in the North Sea, where a meteorological time series is used for comparison. The advantages and limitations of these new techniques, which seem particularly useful for mapping of the regional wind climate, are discussed. Copyright

  11. Wind Speed Estimation and Parametrization of Wake Models for Downregulated Offshore Wind Farms within the scope of PossPOW Project

    NASA Astrophysics Data System (ADS)

    Göçmen Bozkurt, Tuhfe; Giebel, Gregor; Kjølstad Poulsen, Niels; Mirzaei, Mahmood

    2014-06-01

    With increasing installed capacity, wind farms are requested to downregulate more frequently, especially in the offshore environment. Determination and verification of possible (or available) power of downregulated offshore wind farms are the aims of the PossPOW project (see PossPOW.dtu.dk). Two main challenges encountered in the project so far are the estimation of wind speed and the recreation of the flow inside the downregulated wind farm as if it is operating ideally. The rotor effective wind speed was estimated using power, pitch angle and rotational speed as inputs combined with a generic Cp model. The results have been compared with Horns Rev-I dataset and NREL 5MW simulations under both downregulation and normal operation states. For the real-time flow recreation, the GCLarsen single wake model was re-calibrated using a 1-s dataset from Horns Rev and tested for the downregulated period. The re-calibrated model has to be further parametrized to include dynamic effects such as wind direction variability and meandering also considering different averaging time scales before implemented in full scale wind farms.

  12. Using airborne and satellite SAR for wake mapping offshore

    NASA Astrophysics Data System (ADS)

    Christiansen, Merete B.; Hasager, Charlotte B.

    2006-09-01

    Offshore wind energy is progressing rapidly around Europe. One of the latest initiatives is the installation of multiple wind farms in clusters to share cables and maintenance costs and to fully exploit premium wind resource sites. For siting of multiple nearby wind farms, the wind turbine wake effect must be considered. Synthetic aperture radar (SAR) is an imaging remote sensing technique which offers a unique opportunity to describe spatial variations of wind speed offshore. For the first time an airborne SAR instrument was used for data acquisition over a large offshore wind farm. The aim was to identify the turbine wake effect from SAR-derived wind speed maps as a downstream region of reduced wind speed. The aircraft SAR campaign was conducted on 12 October 2003 over the wind farm at Horns Rev in the North Sea. Nearly simultaneous measurements were acquired over the area by the SAR on board the ERS-2 satellite. In addition, meteorological data were collected. Both aircraft and satellite SAR-derived wind speed maps showed significant velocity deficits downstream of the wind farm. Wind speed maps retrieved from aircraft SAR suggested deficits of up to 20% downstream of the last turbine, whereas satellite SAR-derived maps showed deficits of the order of 10%. The difference originated partly from the two different reference methods used for normalization of measured wind speeds. The detected region of reduced wind speed had the same width as the wind turbine array, indicating a low degree of horizontal wake dispersion. The downstream wake extent was approximately 10 km, which corresponds well with results from previous studies and with wake model predictions. Copyright

  13. The Ram's Horn.

    ERIC Educational Resources Information Center

    Rassias, John A., Ed.; And Others

    1983-01-01

    The summer-fall and winter-spring numbers of the journal, "The Ram's Horn," contain these articles: "The Text as Dramatic Departure"; "The Dartmouth Language Outreach Approach to Spanish for Police Action"; "The Dartmouth Intensive Language Model (DILM) in Florida: John Rassias with High School Teachers";…

  14. Horns as particle velocity amplifiers.

    PubMed

    Donskoy, Dimitri M; Cray, Benjamin A

    2011-11-01

    Preliminary measurements and numerical predictions reveal that simple, and relatively small, horns generate remarkable amplification of acoustic particle velocity. For example, below 2 kHz, a 2.5 cm conical horn has a uniform velocity amplification ratio (throat-to-mouth) factor of approximately 3, or, in terms of a decibel level, 9.5 dB. It is shown that the velocity amplification factor depends on the horn's mouth-to-throat ratio as well as, though to a lesser degree, the horn's flare rate. A double horn configuration provides limited additional gain, approximately an increase of up to 25%.

  15. Finline Horn Antennas.

    DTIC Science & Technology

    1985-09-01

    is also given to my second reader, Professor H.M. Lee, for his suggestions on the microstrip to coaxial cable transition for the monopulse comparator...consideranly larger radiating aperture, a highly directive radiation pattern can be achieved. This type of antenna is called an electromagnetic horn. 12...receiver modules are required, as in a pnased array or multichannel direction finding system. B. HELAIED WORK 1. likjA-Fiel Aten Tstn Near-iield antenna

  16. Van Horn folio, Texas

    USGS Publications Warehouse

    Richardson, G.B.

    1914-01-01

    The Van Horn quadrangle is situated in EL Paso and Culberson counties, Tex., about 100 miles southeast of El Paso.  It is bounded by parallels 31° and 31° 30' and meridians 104° 30' and 105° and incluedes 1019 square miles.  This area lies within the Cordilleran region, about midway between Peco River and teh Rio Grande, and forms a part of what is known as trans-Pecos Texas.  

  17. The impact of wakes on power output at large offshore wind farms

    NASA Astrophysics Data System (ADS)

    Barthelmie, R. J.; Frandsen, S.; Hansen, K.; Schepers, G.; Rados, K.; Schlez, W.; Cabezon, D.; Jensen, L.; Neckelmann, S.

    2010-12-01

    The size of planned offshore wind farms is in the range 100 MW to 1 GW requiring tens to hundreds of wind turbines typically arranged in a large array. As wind farms offshore increase in size, one of the research challenges is to model interactions between the individual turbines, the atmosphere and neighbouring turbines to accurately predict power output before wind farm construction in addition to evaluation during the operation phase. The aim of the research described (part of the UpWind project) is to improve wind farm modelling and address the issue of providing more accurate power output predictions accounting for wind turbine wakes. DONG Energy and Vattenfall have allowed data from a number of cases studies to be used in this project. Detailed case studies of power losses due to wakes at the large wind farms at Nysted and Horns Rev have been analysed and are presented. A focus of the data analysis has been to understand the importance of turbulence and atmospheric stability at these offshore sites. It is evident that the magnitude of wake losses is primarily driven by wind speed but that signals from turbine spacing, turbulence and atmospheric stability can be determined. The case studies are simulated with a range of wind farm and computational fluid dynamics (CFD) models. The UpWind project presents a unique platform for model evaluation because the co-operation of a number of groups means that more models can be evaluated on standardised cases. Results shown indicate power losses due to wakes can be modelled, provided that the standard models are subject to some modifications. We also present some of the first full simulations of large offshore wind farms using CFD. Despite this progress, wake modelling of large wind farms is still subject to an unacceptably high degree of uncertainty requiring further work to understand the physical flow processes within and downwind of large wind farms.

  18. Multi-mode horn

    NASA Technical Reports Server (NTRS)

    Neilson, Jeffrey M. (Inventor)

    2002-01-01

    A horn has an input aperture and an output aperture, and comprises a conductive inner surface formed by rotating a curve about a central axis. The curve comprises a first arc having an input aperture end and a transition end, and a second arc having a transition end and an output aperture end. When rotated about the central axis, the first arc input aperture end forms an input aperture, and the second arc output aperture end forms an output aperture. The curve is then optimized to provide a mode conversion which maximizes the power transfer of input energy to the Gaussian mode at the output aperture.

  19. Comment on 'Quantum secret sharing based on reusable Greenberger-Horne-Zeilinger states as secure carriers'

    SciTech Connect

    Gao Fei; Guo Fenzhuo; Wen Qiaoyan; Zhu Fuchen

    2005-09-15

    In a recent paper [S. Bagherinezhad and V. Karimipour, Phys. Rev. A 67, 044302 (2003)], a quantum secret sharing protocol based on reusable Greenberger-Horne-Zeilinger states was proposed. However, in this Comment, it is shown that this protocol is insecure if Eve employs a special strategy to attack.

  20. Stacked Corrugated Horn Rings

    NASA Technical Reports Server (NTRS)

    Sosnowski, John B.

    2010-01-01

    This Brief describes a method of machining and assembly when the depth of corrugations far exceeds the width and conventional machining is not practical. The horn is divided into easily machined, individual rings with shoulders to control the depth. In this specific instance, each of the corrugations is identical in profile, and only differs in diameter and outer profile. The horn is segmented into rings that are cut with an interference fit (zero clearance with all machining errors biased toward contact). The interference faces can be cut with a reverse taper to increase the holding strength of the joint. The taper is a compromise between the interference fit and the clearance of the two faces during assembly. Each internal ring is dipped in liquid nitrogen, then nested in the previous, larger ring. The ring is rotated in the nest until the temperature of the two parts equalizes and the pieces lock together. The resulting assay is stable, strong, and has an internal finish that cannot be achieved through other methods.

  1. Offshore medicine.

    PubMed

    Baker, D

    2001-03-01

    Offshore life can be refreshing for medics who are looking for a little change of pace; however, it is not for everyone. Working offshore can be the easiest or most boring job you'll ever have. It takes a specific type of medic to fit this mold. So, if you are considering a career in the offshore field, take all of the above into consideration. You are not just making a change in jobs, but a change in lifestyle. Once you become accustomed to this lifestyle, it will be hard to go back to the everyday hustle and bustle of the streets. For more information about working offshore, contact Acadian Contract Services at 800/259-333, or visit www.acadian.com.

  2. Offshore Wind Power Integration in severely fluctuating Wind Conditions

    NASA Astrophysics Data System (ADS)

    von Bremen, L.

    2010-09-01

    Strong power fluctuations from offshore wind farms that are induced by wind speed fluctuations pose a severe problem to the save integration of offshore wind power into the power supply system. Experience at the first large-scale offshore wind farm Horns Rev showed that spatial smoothing of power fluctuations within a single wind farm is significantly smaller than onshore results suggest when distributed wind farms of 160 MW altogether are connected to a single point of common-coupling. Wind power gradients larger than 10% of the rated capacity within 5 minutes require large amount of regulation power that is very expensive for the grid operator. It must be noted that a wind speed change of only 0.5m/s result in a wind power change of 10% (within the range of 9-11 m/s where the wind power curve is steepest). Hence, it is very important for the grid operator to know if strong fluctuations are likely or not. Observed weather conditions at the German wind energy research platform FINO1 in the German bight are used to quantify wind fluctuations. With a standard power curve these wind fluctuations are transfered to wind power. The aim is to predict the probability of exceedence of certain wind power gradients that occur in a time interval of e.g. 12 hours. During 2006 and 2009 the distribution of wind power fluctuations looks very similar giving hope that distinct atmospheric processes can be determined that act as a trigger. Most often high wind power fluctuations occur in a range of wind speeds between 9-12 m/s as can be expected from the shape of the wind power curve. A cluster analysis of the 500 hPa geopotential height to detect predominant weather regimes shows that high fluctuations are more likely in north-western flow. It is shown that most often high fluctuations occur in non-stable atmospheric stratification. The description of stratification by means of the vertical gradient of the virtual potential temperature is chosen to be indicative for convection, i

  3. Ancient celtic horns

    NASA Astrophysics Data System (ADS)

    Campbell, Murray

    2002-11-01

    There is considerable evidence from iconographic and documentary sources that musical lip-reed instruments were important in the early celtic communities of Scotland and Ireland. In recent years several studies have been undertaken with the aim of gaining a better understanding of the musical nature of these ancient horns, and of their place in the life and culture of the time. A valuable source of tangible evidence is to be found in the archaeological remains deposited across Scotland and the whole of Ireland. A project is now under way, under the auspices of the Kilmartin House Trust and the general direction of John Purser, which has brought together an international team of musicians, craftsmen, archaeologists, musicologists and physicists with the aim of analyzing ancient musical artifacts, reconstructing some of the original instruments, and analyzing the sounds they produce. This paper describes acoustical studies carried out on a number of recent reconstructions of wooden and bronze instruments, and discusses the role of acoustics in this type of investigation. [Work supported by Sciart and EPSRC.

  4. Keynote Address: Rev. Mark Massa

    ERIC Educational Resources Information Center

    Massa, Mark S.

    2011-01-01

    Rev. Mark S. Massa, S.J., is the dean and professor of Church history at the School of Theology and Ministry at Boston College. He was invited to give a keynote to begin the third Catholic Higher Education Collaborative Conference (CHEC), cosponsored by Boston College and Fordham University. Fr. Massa's address posed critical questions about…

  5. Maximal violation of the Clauser-Horne-Shimony-Holt inequality for two qutrits

    SciTech Connect

    Fu, Li-Bin; Zhao, Xian-Geng; Chen, Jing-Ling

    2003-08-01

    The Bell-Clauser-Horne-Shimony-Holt (BCHSH) inequality (in terms of correlation functions) of two qutrits is studied in detail by employing tritter measurements. A uniform formula for the maximum value of this inequality for tritter measurements is obtained. Based on this formula, we show that nonmaximally entangled states violate the BCHSH inequality more strongly than the maximally entangled one. This result is consistent with what was obtained by Acin et al. [Phys. Rev. A 65, 052325 (2002)] using the Bell-Clauser-Horne inequality (in terms of probabilities)

  6. Using Synthetic Aperture Radar Wind Measurements to support Offshore Wind Parks

    NASA Astrophysics Data System (ADS)

    Schneiderhan, T.; Lehner, S.; Horstmann, J.; Koch, W.; Schulz-Stellenfleth, J.

    2003-04-01

    In all countries with shallow coastal waters and a strong mean wind speed offshore wind parks are planned and built. The fast development of wind energy production in Europe led to an installation of more than 18 000 MW by the end of the year 2001. The installed offshore power up to date is about 100 MW. In the near future many projects for wind farms with an output of more than 5000 MW are planned. Some of these projects are already under construction. Offshore wind parks are showing a big potential for future energy production and solving ecological problems in reducing the CO^2 output. The construction and maintenance of offshore wind parks has to face the tough environmental conditions of the open sea resulting extensive maintenance and money. Therefore reliable forecast in particular of the wind and the ocean wave fields is essential. Space borne SAR data as acquired by the ERS satellites or the new ENVISAT satellite, launched in March 2002, provide two dimensional wind fields with a sub-kilometre resolution and a coverage of up to 500 by 500 km in the wide swath mode. They are thus ideally suited to investigate the spatial fine structure like e.g. turbulence in the wake of wind parks, which is an important factor in the optimal siting of wind farms. Due to their high coverage and resolution SAR data can provide information on the impact of the single turbines on the wind field experienced by the neighbouring turbines as well as the effect of the whole wind park on the local climate. This study shows the potential of two dimensional high resolution wind fields measured with space borne synthetic aperture radar to support the construction and operation of wind farms. The data can be used to minimize fatigue loading due to wind gusts as well as to provide short term power forecasts in order to optimise the power output. Examples of wind fields around the already existing offshore wind parks Utgrunden (South of Sweden) and Horns Rev (West of Denmark) and the

  7. Smarter offshoring.

    PubMed

    Farrell, Diana

    2006-06-01

    During the past 15 years, companies have flocked to a handful of cities in India and Eastern Europe for offshore service functions. As a result, the most popular sites are now overheating: Demand for young professionals is outstripping supply, wages and turnover are soaring, and overburdened infrastructure systems are struggling to serve the explosive growth. The happy news is that the tight labor markets in the well-known hot spots are the exceptions, not the rule. Many attractive alternatives are emerging around the world. According to a McKinsey Global Institute study, more than 90% of the vast and rapidly growing pool of university-educated people suitable for work in multinationals are located outside the current hot spot cities. For instance, Morocco is now home to offshore centers for French and Spanish companies requiring fluent speakers of their home languages. Neighboring Tunisia has used its modern infrastructure, business-friendly regulations, and stable, low-cost workforce to attract companies such as Siemens and Wanadoo. Vietnam offers university graduates who have strong mathematics skills; speak French, English, German, or Russian; and do not demand high wages. The problems facing the hot spots, coupled with the emergence of many more countries able and willing to provide offshore services, mean that picking a site has become more complicated. In choosing a location, companies will have to focus less on low wages and much more on other ways that candidate cities can fulfill their business needs. They will have to be much more rigorous in articulating precisely what they require from an offshore location. That means evaluating their unique needs on a range of dimensions and understanding how alternative locations can meet those needs for the foreseeable future.

  8. Stable complex formation between HIV Rev and the nucleosome assembly protein, NAP1, affects Rev function

    SciTech Connect

    Cochrane, Alan; Murley, Laura Lea; Gao Mian; Wong, Raymond; Clayton, Kiera; Brufatto, Nicole; Canadien, Veronica; Mamelak, Daniel; Chen, Tricia; Richards, Dawn; Zeghouf, Mahel; Greenblatt, Jack; Burks, Christian; Frappier, Lori

    2009-05-25

    The Rev protein of HIV-1 is essential for HIV-1 proliferation due to its role in exporting viral RNA from the nucleus. We used a modified version of tandem affinity purification (TAP) tagging to identify proteins interacting with HIV-1 Rev in human cells and discovered a prominent interaction between Rev and nucleosome assembly protein 1 (Nap1). This interaction was also observed by specific retention of Nap1 from human cell lysates on a Rev affinity column. Nap1 was found to bind Rev through the Rev arginine-rich domain and altered the oligomerization state of Rev in vitro. Overexpression of Nap1 stimulated the ability of Rev to export RNA, reduced the nucleolar localization of Rev, and affected Rev nuclear import rates. The results suggest that Nap-1 may influence Rev function by increasing the availability of Rev.

  9. Successful outcome in preeclamptic rudimentary horn pregnancy.

    PubMed

    Sinha, Ruchi; Sachan, Shikha; Khanna, Anuradha

    2011-01-01

    Unicornuate uterus with rudimentary horn is an uncommon type of mullerian duct malformation associated with various gynecological and obstetrical complications. Rudimentary horn pregnancy is a rare entity and the majority have rupture of gravid horn leading to maternal and fetal morbidity and mortality. A case of rudimentary horn pregnancy at 32 weeks and 6 days with pregnancy induced hypertension is reported where proper management results in successful pregnancy outcome.

  10. Wind Speed Estimation and Wake model Re-calibration for Downregulated Offshore Wind Farms

    NASA Astrophysics Data System (ADS)

    Göçmen Bozkurt, Tuhfe; Giebel, Gregor; Kjølstad Poulsen, Niels; Réthoré, Pierre-Elouan; Mirzaei, Mahmood

    2014-05-01

    In recent years, the wind farm sizes have increased tremendously and with increasing installed capacity, the wind farms are requested to downregulate from their maximum possible power more frequently, especially in the offshore environment. Determination of the possible (or available) power is crucial not only because the reserve power has considerable market value but also for wind farm developers to be properly compensated for the loss during downregulation. While the available power calculation is straightforward for a single turbine, it gets rather complicated for the whole wind farm due to the change in the wake characteristics. In fact, the wake losses generated by the upstream turbine(s) decrease during downregulation and the downstream turbines therefore see more wind compared to the normal operation case. Currently, the Transmission System Operators (TSOs) have no real way to determine exactly the available power of a whole wind farm which is downregulated. Therefore, the PossPOW project aims to develop a verified and internationally accepted way to determine the possible power of a down-regulated offshore wind farm. The first phase of the project is to estimate the rotor effective wind speed. Since the nacelle anemometers are not readily available and are known to have reliability issues, the proposed method is to use power, pitch angle and rotational speed as inputs and combine it with a generic Cp model to estimate the wind speed. The performance of the model has been evaluated for both normal operation and downregulation periods using two different case studies: Horns Rev-I wind farm and NREL 5MW single turbine. During downregulation, the wake losses are not as severe and the velocity deficits at the downstream turbines are smaller as if also the wake is "downregulated". On the other hand, in order to calculate the available power, the wakes that would have been produced normally (if the turbines were not curtailed) are of importance, not the

  11. Folded Resonant Horns for Power Ultrasonic Applications

    NASA Technical Reports Server (NTRS)

    Sherrit, Stewart; Askins, Stephen; Gradziel, Michael; Bao, Xiaoqi; Chang, Zensheu; Dolgin, Benjamin; Bar-Cohen, Yoseph; Peterson, Tom

    2003-01-01

    Folded horns have been conceived as alternatives to straight horns used as resonators and strain amplifiers in power ultrasonic systems. Such systems are used for cleaning, welding, soldering, cutting, and drilling in a variety of industries. In addition, several previous NASA Tech Briefs articles have described instrumented drilling, coring, and burrowing machines that utilize combinations of sonic and ultrasonic vibrational actuation. The main advantage of a folded horn, relative to a straight horn of the same resonance frequency, is that the folded horn can be made shorter (that is, its greatest linear dimension measured from the outside can be made smaller). Alternatively, for a given length, the resonance frequency can be reduced. Hence, the folded-horn concept affords an additional degree of design freedom for reducing the length of an ultrasonic power system that includes a horn.

  12. Novel Horn Designs for Power Ultrasonics

    NASA Technical Reports Server (NTRS)

    Sherrit, Stewart; Badescu, M.; Bao, X.; Bar-Cohen, Y.; Chang, Z.

    2004-01-01

    Ultrasonic horns are used in a variety of industrial and medical applications. At JPL a rock-sampling tool based on an ultrasonic horn was developed to drill, abrade and core rock samples including hard basalts. This device is an impact device, which uses ultrasonic vibratlons that occur at the horn tip to produce a sonic resonance with the aid of a loosely connected mass. Although standard horns are found in many current industrial designs they suffer from a few key limitations when used for USDC applications. Manufacturing a horn requires turning down stock material (e.g. Titanium) from the larger outer diameter to the horn tip diameter, and this process is both time consuming and wasteful. In this paper, we present novel horn designs that are specifically designed for impact applications as the USDC. One such design addressed the excasive length that is involved with the use of the horn limiting its applications when system dimensions are constrained. For this purpose, a folded horn design was conceived that reduces the overall length of the resonator (physical length) but maintains or increases the acoustic length. Initial experiments with horns having such P design indicate that the tip displacement can be further adjusted by phasing the bending displacements and the extensional displacements. Another conceived horn design is the 'dog bone' horn that uses an end mass on the horn tip io increase the impact efficiency of the horn. In this paper, the experimental results for these novel born designs are presented and compared to the results predicted by theory.

  13. Novel Horn Designs for Power Ultrasonics

    NASA Technical Reports Server (NTRS)

    Sherrit, Stewart; Badescu, M.; Bao, X.; Bar-Cohen, Y.; Chang, Z.

    2004-01-01

    Ultrasonic horns are used in a variety of industrial and medical applications. At JPL a rock-sampling tool based on an ultrasonic horn was developed to drill, abrade and core rock samples including hard basalts. This device is an impact device, which uses ultrasonic vibratlons that occur at the horn tip to produce a sonic resonance with the aid of a loosely connected mass. Although standard horns are found in many current industrial designs they suffer from a few key limitations when used for USDC applications. Manufacturing a horn requires turning down stock material (e.g. Titanium) from the larger outer diameter to the horn tip diameter, and this process is both time consuming and wasteful. In this paper, we present novel horn designs that are specifically designed for impact applications as the USDC. One such design addressed the excasive length that is involved with the use of the horn limiting its applications when system dimensions are constrained. For this purpose, a folded horn design was conceived that reduces the overall length of the resonator (physical length) but maintains or increases the acoustic length. Initial experiments with horns having such P design indicate that the tip displacement can be further adjusted by phasing the bending displacements and the extensional displacements. Another conceived horn design is the 'dog bone' horn that uses an end mass on the horn tip io increase the impact efficiency of the horn. In this paper, the experimental results for these novel born designs are presented and compared to the results predicted by theory.

  14. Multifrequency broadband polarized horn antenna

    NASA Technical Reports Server (NTRS)

    Green, K. A. (Inventor)

    1981-01-01

    A corrugated conical horn antenna is simultaneously fed a multiplicity of signals, two for each of five frequencies, with each of a pair of signals fed in each of two orthogonal planes for excitation of a desired spherical hybrid mode. The lowest frequency is fed into the horn through orthogonal pairs of colinear slots, each pair being fed by coaxial tee power dividers. Other signals are fed through a circular waveguide connected to the vertex. The highest frequency signals are fed through orthogonal ports near the far end of the circular waveguide. The intermediate frequency signals are fed through orthogonal ports spaced along the waveguide. Filtering is incorporated for each to maintain isolation and low insertion loss.

  15. SIW Based Wideband Horn Antenna

    NASA Astrophysics Data System (ADS)

    Patel, Amit, Dr.; Vala, Alpesh; Goswami, Riddhi; Mahant, Keyur

    2017-08-01

    In this paper, we have proposed CSRR (complementary split ring resonator) loaded Substrate Integrated Waveguide (SIW) horn antenna. The whole system is designed on a single substrate, having advantages of small size, low profile, and low cost, etc. The design process and simulation results of a CSRR-loaded SIW horn antenna at K-band and Ka-band are presented. The proposed antenna is an outstanding choice for K, Ka bands and even higher frequency synthesis. It has well-behaved gain and suitable reflection coefficient value less than 1.5 (-10dB S11 and VSWR<1.5). The simulated gain of antenna attains 7.48±1dB over majority of the bandwidth and with radiation efficiency of 85%. The simulation has been done using full-wave package, High Frequency Structure Simulator Software (HFSS) based on Finite element method (FEM).

  16. Printed sectoral horn power combiner

    NASA Astrophysics Data System (ADS)

    Boccia, Luigi; Emanuele, Antonio; Shamsafar, Alireza; Arnieri, Emilio; Amendola, Giandomenico

    2015-02-01

    In this work, it is presented a new configuration of planar power combiner/divider based on an H-plane sectoral horn antenna. This component is proposed to realise the basic building blocks of printed power-combining amplifiers. It will be shown how the sectoral horn elements can be implemented on substrate integrated waveguide and multilayer printed circuit board technologies, thus obtaining a high integration level. In the following, the design procedure will be described reporting an example of an 11-stage power divider/combiner in C-band. A prototype has been fabricated, and the measured results compared with the numerical model. Experimental results are in good agreement with theoretical expectations showing a single-stage efficiency of about 90% and a bandwidth of 40%.

  17. Multi-mode horn antenna simulation

    NASA Technical Reports Server (NTRS)

    Dod, L. R.; Wolf, J. D.

    1980-01-01

    Radiation patterns were computed for a circular multimode horn antenna using waveguide electric field radiation expressions. The circular multimode horn was considered as a possible reflector feed antenna for the Large Antenna Multifrequency Microwave Radiometer (LAMMR). This horn antenna uses a summation of the TE sub 11 deg and TM sub 11 deg modes to generate far field primary radiation patterns with equal E and H plane beamwidths and low sidelobes. A computer program for the radiation field expressions using the summation of waveguide radiation modes is described. The sensitivity of the multimode horn antenna radiation patterns to phase variations between the two modes is given. Sample radiation pattern calculations for a reflector feed horn for LAMMR are shown. The multimode horn antenna provides a low noise feed suitable for radiometric applications.

  18. Miniaturization of planar horn motors

    NASA Astrophysics Data System (ADS)

    Sherrit, Stewart; Ostlund, Patrick N.; Chang, Zensheu; Bao, Xiaoqi; Bar-Cohen, Yoseph; Widholm, Scott E.; Badescu, Mircea

    2012-04-01

    There is a great need for compact, efficient motors for driving various mechanisms including robots or mobility platforms. A study is currently underway to develop a new type of piezoelectric actuators with significantly more strength, low mass, small footprint, and efficiency. The actuators/motors utilize piezoelectric actuated horns which have a very high power density and high electromechanical conversion efficiency. The horns are fabricated using our recently developed novel pre-stress flexures that make them thermally stable and increases their coupling efficiency. The monolithic design and integrated flexures that pre-stresses the piezoelectric stack eliminates the use of a stress bolt. This design allows embedding solid-state motors and actuators in any structure so that the only macroscopically moving parts are the rotor or the linear translator. The developed actuator uses a stack/horn actuation and has a Barth motor configuration, which potentially generates very large torque and speeds that do not require gearing. Finite element modeling and design tools were investigated to determine the requirements and operation parameters and the results were used to design and fabricate a motor. This new design offers a highly promising actuation mechanism that can potentially be miniaturized and integrated into systems and structures. It can be configured in many shapes to operate as multi-degrees of freedom and multi-dimensional motors/actuators including unidirectional, bidirectional, 2D and 3D. In this manuscript, we are reporting the experimental measurements from a bench top design and the results from the efforts to miniaturize the design using 2×2×2 mm piezoelectric stacks integrated into thin plates that are of the order of 3 × 3 × 0.2 cm.

  19. Miniaturization of Planar Horn Motors

    NASA Technical Reports Server (NTRS)

    Sherrit, Stewart; Ostlund, Patrick N.; Chang, Zensheu; Bao, Xiaoqi; Bar-Cohen, Yoseph; Widholm, Scott E.; Badescu, Mircea

    2012-01-01

    There is a great need for compact, efficient motors for driving various mechanisms including robots or mobility platforms. A study is currently underway to develop a new type of piezoelectric actuators with significantly more strength, low mass, small footprint, and efficiency. The actuators/motors utilize piezoelectric actuated horns which have a very high power density and high electromechanical conversion efficiency. The horns are fabricated using our recently developed novel pre-stress flexures that make them thermally stable and increases their coupling efficiency. The monolithic design and integrated flexures that pre-stresses the piezoelectric stack eliminates the use of stress bolt. This design allows embedding solid-state motors and actuators in any structure so that the only macroscopically moving parts are the rotor or the linear translator. The developed actuator uses a stack/horn actuation and has a Barth motor configuration, which potentially generates very large torque and speeds that do not require gearing. Finite element modeling and design tools were investigated to determine the requirements and operation parameters and the results were used to design and fabricate a motor. This new design offers a highly promising actuation mechanism that can potentially be miniaturized and integrated into systems and structures. It can be configured in many shapes to operate as multi-degrees of freedom and multi-dimensional motors/actuators including unidirectional, bidirectional, 2D and 3D. In this manuscript, we are reporting the experimental measurements from a bench top design and the results from the efforts to miniaturize the design using 2x2x2 mm piezoelectric stacks integrated into thin plates that are of the order of3 x 3x 0.2 cm.

  20. Miniaturization of Planar Horn Motors

    NASA Technical Reports Server (NTRS)

    Sherrit, Stewart; Ostlund, Patrick N.; Chang, Zensheu; Bao, Xiaoqi; Bar-Cohen, Yoseph; Widholm, Scott E.; Badescu, Mircea

    2012-01-01

    There is a great need for compact, efficient motors for driving various mechanisms including robots or mobility platforms. A study is currently underway to develop a new type of piezoelectric actuators with significantly more strength, low mass, small footprint, and efficiency. The actuators/motors utilize piezoelectric actuated horns which have a very high power density and high electromechanical conversion efficiency. The horns are fabricated using our recently developed novel pre-stress flexures that make them thermally stable and increases their coupling efficiency. The monolithic design and integrated flexures that pre-stresses the piezoelectric stack eliminates the use of stress bolt. This design allows embedding solid-state motors and actuators in any structure so that the only macroscopically moving parts are the rotor or the linear translator. The developed actuator uses a stack/horn actuation and has a Barth motor configuration, which potentially generates very large torque and speeds that do not require gearing. Finite element modeling and design tools were investigated to determine the requirements and operation parameters and the results were used to design and fabricate a motor. This new design offers a highly promising actuation mechanism that can potentially be miniaturized and integrated into systems and structures. It can be configured in many shapes to operate as multi-degrees of freedom and multi-dimensional motors/actuators including unidirectional, bidirectional, 2D and 3D. In this manuscript, we are reporting the experimental measurements from a bench top design and the results from the efforts to miniaturize the design using 2x2x2 mm piezoelectric stacks integrated into thin plates that are of the order of3 x 3x 0.2 cm.

  1. Horn fly, Haematobia irritans irritans L., overwintering

    USDA-ARS?s Scientific Manuscript database

    Putative diapause in the horn fly, Haematobia irritans irritans (L.), has frequently been assumed as the pest's mode for overwinter survival from the tropics to temperate regions of northern and southern hemispheres. Examination of the scientific literature indicates that putative horn fly diapause ...

  2. Teleportation of continuous variable multimode Greeberger Horne Zeilinger entangled states

    NASA Astrophysics Data System (ADS)

    He, Guangqiang; Zhang, Jingtao; Zeng, Guihua

    2008-11-01

    Quantum teleportation protocols of continuous variable (CV) Greeberger-Horne-Zeilinger (GHZ) and Einstein-Podolsky-Rosen (EPR) entangled states are proposed, and are generalized to teleportation of arbitrary multimode GHZ entangled states described by Van Loock and Braunstein (2000 Phys. Rev. Lett. 84 3482). Each mode of a multimode entangled state is teleported using a CV EPR entangled pair and classical communication. The analytical expression of fidelity for the multimode Gaussian states which evaluates the teleportation quality is presented. The analytical results show that the fidelity is a function of both the squeezing parameter r, which characterizes the multimode entangled state to be teleported, and the channel parameter p, which characterizes the EPR pairs shared by Alice and Bob. The fidelity increases with increasing p, but decreases with increasing r, i.e., it is more difficult to teleport the more perfect multimode entangled states. The entanglement degree of the teleported multimode entangled states increases with increasing both r and p. In addition, the fact is proved that our teleportation protocol of EPR entangled states using parallel EPR pairs as quantum channels is the best case of the protocol using four-mode entangled states (Adhikari et al 2008 Phys. Rev. A 77 012337).

  3. Rupture rudimentary horn pregnancy at 31 week.

    PubMed

    Al Qarni, Abdullah A; Al-Braikan, Nawal; Al-Hanbali, Moh'd M; Alharmaly, Abdullah H

    2017-02-01

    Rudimentary horn could be a rare congenital uterine anomalies result from incomplete fusion of the two Müllerian ducts throughout embryo-genesis. Pregnancy in an exceedingly rudimentary horn is rare and typically terminates in rupture throughout the late of first or second trimester of pregnancy. We tend to present a rare case of a gravida within the trimester (31 weeks) presented with complain of abdominal pain, nausea, and vomit for 3 days. On presentation, the patient was pale and irritable. Ultrasound scan showed fetus at (30 weeks) with cardiac activity. Cardiotocography reactive. Incision showed horned uterus with pregnancy in rudimentary left horn that was ruptured. Alive fetus was delivered. The rudimentary left horn was excised. The patient was advised to not get pregnant for 3 years. Within one year, the patient came to hospital in active labor at (37 weeks). This case emphasizes the importance of fine antepartum care to avoid morbidity and mortality.

  4. Rupture rudimentary horn pregnancy at 31 week

    PubMed Central

    Al Qarni, Abdullah A.; Al-Braikan, Nawal; Al-Hanbali, Moh’d M.; Alharmaly, Abdullah H.

    2017-01-01

    Rudimentary horn could be a rare congenital uterine anomalies result from incomplete fusion of the 2 Müllerian ducts throughout embryo-genesis. Pregnancy in an exceedingly rudimentary horn is rare and typically terminates in rupture throughout the late of first or second trimester of pregnancy. We tend to present a rare case of a gravida within the trimester (31 weeks) presented with complain of abdominal pain, nausea, and vomit for 3 days. On presentation, the patient was pale and irritable. Ultrasound scan showed fetus at (30 weeks) with cardiac activity. Cardiotocography reactive. Incision showed horned uterus with pregnancy in rudimentary left horn that was ruptured. Alive fetus was delivered. The rudimentary left horn was excised. The patient was advised to not get pregnant for 3 years. Within one year, the patient came to hospital in active labor at (37 weeks). This case emphasizes the importance of fine antepartum care to avoid morbidity and mortality. PMID:28133695

  5. Horn growth patterns in Alpine chamois.

    PubMed

    Corlatti, Luca; Gugiatti, Alessandro; Imperio, Simona

    2015-06-01

    The analysis of horn growth may provide important information about the allocation of metabolic resources to secondary sexual traits. Depending on the selective advantages offered by horn size during intra- and inter-specific interactions, ungulates may show different investment in horn development, and growth variations within species may be influenced by several parameters, such as sex, age, or resource availability. We investigated the horn growth patterns in two hunted populations of Alpine chamois (Rupicapra r. rupicapra) in the Central Italian Alps. We tested the role of individual heterogeneity on the growth pattern and explored the variation in annulus length as a function of different factors (sex, age, hunting location, cohort). We then investigated the mechanisms underlying horn growth trajectories to test for the occurrence of compensatory or recovery growth and their potential differences between sexes and populations. Annulus length varied as a function of sex, age of individuals and, marginally, hunting location; no effect of cohort or individual heterogeneity was detected. Male and female chamois showed compensatory horn growth within the first 5½ years of life, though the partial convergence of horn trajectories in chamois suggests that this mechanisms would best be described as 'recovery growth'. Compensation rates were greater in males than in females, while only compensatory growth rates up to 2½ years of age were different in the two populations. Besides confirming the sex- and age-dependent pattern of horn development, our study suggests that the mechanism of recovery growth supports the hypothesis of horn size as a weakly selected sexual trait in male and female chamois. Furthermore, the greater compensation rates in horn growth shown by male chamois possibly suggest selective effects of hunting on age at first reproduction, while different compensation rates between populations may suggest the occurrence of some plasticity in resource

  6. Optimized Chemical Probes for REV-ERBα

    PubMed Central

    Trump, Ryan P.; Bresciani, Stefano; Cooper, Anthony W. J.; Tellam, James P.; Wojno, Justyna; Blaikley, John; Orband-Miller, Lisa A.; Kashatus, Jennifer A.; Dawson, Helen C.; Loudon, Andrew; Ray, David; Grant, Daniel; Farrow, Stuart N.; Willson, Timothy M.; Tomkinson, Nicholas C. O.

    2015-01-01

    REV-ERBα has emerged as an important target for regulation of circadian rhythm and its associated physiology. Herein, we report on the optimization of a series of REV-ERBα agonists based on GSK4112 (1) for potency, selectivity, and bioavailability. Potent REV-ERBα agonists 4, 10, 16, and 23 are detailed for their ability to suppress BMAL and IL-6 expression from human cells while also demonstrating excellent selectivity over LXRα. Amine 4 demonstrated in vivo bioavailability after either IV or oral dosing. PMID:23656296

  7. Optimized chemical probes for REV-ERBα.

    PubMed

    Trump, Ryan P; Bresciani, Stefano; Cooper, Anthony W J; Tellam, James P; Wojno, Justyna; Blaikley, John; Orband-Miller, Lisa A; Kashatus, Jennifer A; Boudjelal, Mohamed; Dawson, Helen C; Loudon, Andrew; Ray, David; Grant, Daniel; Farrow, Stuart N; Willson, Timothy M; Tomkinson, Nicholas C O

    2013-06-13

    REV-ERBα has emerged as an important target for regulation of circadian rhythm and its associated physiology. Herein, we report on the optimization of a series of REV-ERBα agonists based on GSK4112 (1) for potency, selectivity, and bioavailability. (1) Potent REV-ERBα agonists 4, 10, 16, and 23 are detailed for their ability to suppress BMAL and IL-6 expression from human cells while also demonstrating excellent selectivity over LXRα. Amine 4 demonstrated in vivo bioavailability after either iv or oral dosing.

  8. Cutaneous horns: clues to invasive squamous cell carcinoma being present in the horn base.

    PubMed

    Pyne, John; Sapkota, Devendra; Wong, Jian Cheng

    2013-04-01

    Cutaneous horns usually develop on a keratinocytic base with the histopathology on a spectrum ranging from benign keratosis through to invasive squamous cell carcinoma (SCC). Some features of horns are easily identified using dermatoscopy. To investigate if specific clinical or dermatoscopy features of horns correlate with the histopathology in the base of the horn. Consecutive horn cases (n=163) were assessed prospectively in vivo for horn height, terrace morphology and base erythema using a Heine Delta 20 dermatoscope. Cases with potentially confounding influences were excluded. A history of horn pain or pain on palpation was also recorded. Benign keratosis (n = 49), actinic keratosis (n = 21), SCC in situ (n = 37) and invasive SCC (n = 56) were recorded. An invasive SCC presenting as a horn as most likely to have a height less than the base diameter, 66% (37/56). Compared to the other study entities, invasive SCC tends to have less terrace morphology (P<0.05), a higher incidence of base erythema (P<0.05) and more pain (P<0.01). Data categories did not include anatomic site or horn growth rates. Excision selection bias favored the incidence of invasive SCC. Horns presenting on an invasive SCC base are more likely to have a height less than the diameter of the base, not to have terrace morphology, to have an erythematous base and to be painful.

  9. Olfactory coding in the honeybee lateral horn.

    PubMed

    Roussel, Edith; Carcaud, Julie; Combe, Maud; Giurfa, Martin; Sandoz, Jean-Christophe

    2014-03-03

    Olfactory systems dynamically encode odor information in the nervous system. Insects constitute a well-established model for the study of the neural processes underlying olfactory perception. In insects, odors are detected by sensory neurons located in the antennae, whose axons project to a primary processing center, the antennal lobe. There, the olfactory message is reshaped and further conveyed to higher-order centers, the mushroom bodies and the lateral horn. Previous work has intensively analyzed the principles of olfactory processing in the antennal lobe and in the mushroom bodies. However, how the lateral horn participates in olfactory coding remains comparatively more enigmatic. We studied odor representation at the input to the lateral horn of the honeybee, a social insect that relies on both floral odors for foraging and pheromones for social communication. Using in vivo calcium imaging, we show consistent neural activity in the honeybee lateral horn upon stimulation with both floral volatiles and social pheromones. Recordings reveal odor-specific maps in this brain region as stimulations with the same odorant elicit more similar spatial activity patterns than stimulations with different odorants. Odor-similarity relationships are mostly conserved between antennal lobe and lateral horn, so that odor maps recorded in the lateral horn allow predicting bees' behavioral responses to floral odorants. In addition, a clear segregation of odorants based on pheromone type is found in both structures. The lateral horn thus contains an odor-specific map with distinct representations for the different bee pheromones, a prerequisite for eliciting specific behaviors.

  10. The SeaHorn Verification Framework

    NASA Technical Reports Server (NTRS)

    Gurfinkel, Arie; Kahsai, Temesghen; Komuravelli, Anvesh; Navas, Jorge A.

    2015-01-01

    In this paper, we present SeaHorn, a software verification framework. The key distinguishing feature of SeaHorn is its modular design that separates the concerns of the syntax of the programming language, its operational semantics, and the verification semantics. SeaHorn encompasses several novelties: it (a) encodes verification conditions using an efficient yet precise inter-procedural technique, (b) provides flexibility in the verification semantics to allow different levels of precision, (c) leverages the state-of-the-art in software model checking and abstract interpretation for verification, and (d) uses Horn-clauses as an intermediate language to represent verification conditions which simplifies interfacing with multiple verification tools based on Horn-clauses. SeaHorn provides users with a powerful verification tool and researchers with an extensible and customizable framework for experimenting with new software verification techniques. The effectiveness and scalability of SeaHorn are demonstrated by an extensive experimental evaluation using benchmarks from SV-COMP 2015 and real avionics code.

  11. Microstructure and mechanical properties of sheep horn.

    PubMed

    Zhu, Bing; Zhang, Ming; Zhao, Jian

    2016-07-01

    The sheep horn presents outstanding mechanical properties of impact resistance and energy absorption, which suits the need of the vehicle bumper design, but the mechanism behind this phenomenon is less investigated. The microstructure and mechanical properties of the sheep horn of Small Tailed Han Sheep (Ovis aries) living in northeast China were investigated in this article. The effect of sampling position and orientation of the sheep horn sheath on mechanical properties were researched by tensile and compression tests. Meanwhile, the surface morphology and microstructure of the sheep horn were observed using scanning electron microscopy (SEM). The formation mechanism of the mechanical properties of the sheep horn was investigated by biological coupling analysis. The analytical results indicated that the outstanding mechanical properties of the sheep horn are determined by configuration, structure, surface morphology and material coupling elements. These biological coupling elements make the sheep horn possess super characteristics of crashworthiness and energy absorption through the internal coupling mechanism. We suppose that these findings would make a difference in vehicle bumper design. Microsc. Res. Tech. 79:664-674, 2016. © 2016 Wiley Periodicals, Inc. © 2016 Wiley Periodicals, Inc.

  12. Audibility of train horns in passenger vehicles.

    PubMed

    Dolan, Thomas G; Rainey, Jess E

    2005-01-01

    Studies of accident rates associated with train horn bans indicate that motorists rely on horns to warn them of approaching trains. However, researchers have not yet established the levels of horn sounds necessary for detection at railroad crossings. The purpose of this study was to obtain baseline measures of the auditory component of the motorist's detection task. Horn sounds recorded in three test vehicles were presented to 20 normal-hearing listeners in quiet and in four types of vehicle interior noise: engine idling, ventilation fan off; engine idling, fan on; vehicle moving at 30 miles/hr (mph), fan off; and vehicle moving at 30 mph, fan on. Thresholds of the horn sounds were determined by an adaptive procedure. Mean thresholds were lowest in quiet (1.8-4.4 dBA) and highest for the 30-mph, fan-on condition (49.7-58.4 dBA). Mean horn thresholds for all 12 noise conditions were more than 10 dB below the overall level of the vehicle interior noise. Our data are compared with those of previous studies and their implications are discussed. Actual or potential applications of this research include the establishment of a lower limit of signal-to-noise ratios required for the detection of horn sounds at highway-rail crossings.

  13. An Annotated Guide and Interactive Database for Solo Horn Repertoire

    ERIC Educational Resources Information Center

    Schouten, Sarah

    2012-01-01

    Given the horn's lengthy history, it is not surprising that many scholars have examined the evolution of the instrument from the natural horn to the modern horn and its expansive repertoire. Numerous dissertations, theses, and treatises illuminate specific elements of the horn's solo repertoire; however, no scholar has produced a…

  14. An Annotated Guide and Interactive Database for Solo Horn Repertoire

    ERIC Educational Resources Information Center

    Schouten, Sarah

    2012-01-01

    Given the horn's lengthy history, it is not surprising that many scholars have examined the evolution of the instrument from the natural horn to the modern horn and its expansive repertoire. Numerous dissertations, theses, and treatises illuminate specific elements of the horn's solo repertoire; however, no scholar has produced a…

  15. Radiation from Axisymmetric Waveguide Fed Horns

    NASA Technical Reports Server (NTRS)

    Chinn, G. C.; Hoppe, D. J.; Epp, L. W.

    1995-01-01

    Return losses and radiation patterns for axisymmetric waveguide fed horns are calculated with the finite element method (FEM) in conjunction with the method of moments (MoM) and the mode matching technique (MM).

  16. 802GHz integrated horn antennas imaging array

    NASA Technical Reports Server (NTRS)

    Ali-Ahmad, Walid Y.; Rebeiz, Gabriel M.; Dave, Hemant; Chin, Gordon

    1991-01-01

    Pattern measurements at 802GHz of a single element in 256-element integrated horn imaging array are presented. The integrated-horn antenna consists of a dipole-antenna suspended on a 1-micron dielectric membrane inside a pyramidal cavity etched in silicon. The theoretical far-field patterns, calculated using reciprocity and Floquet-modes representation of the free-space field, agree well with the measured far-field patterns at 802GHz. The associated directivity for a 1.40 lambda horn aperture, calculated from the measured E and H-plane patterns is 12.3dB + or - 0.2dB. This work demonstrates that high-efficiency integrated-horn antennas are easily scalable to terahertz frequencies and could be used for radio-astronomical and plasma-diagnostic applications.

  17. Radiation from Axisymmetric Waveguide Fed Horns

    NASA Technical Reports Server (NTRS)

    Chinn, G. C.; Hoppe, D. J.; Epp, L. W.

    1995-01-01

    Return losses and radiation patterns for axisymmetric waveguide fed horns are calculated with the finite element method (FEM) in conjunction with the method of moments (MoM) and the mode matching technique (MM).

  18. Planar Rotary Piezoelectric Motor Using Ultrasonic Horns

    NASA Technical Reports Server (NTRS)

    Sherrit, Stewart; Bao, Xiaoqi; Badescu, Mircea; Bar-Cohen, Yoseph; Geiyer, Daniel; Ostlund, Patrick N.; Allen, Phillip

    2011-01-01

    A motor involves a simple design that can be embedded into a plate structure by incorporating ultrasonic horn actuators into the plate. The piezoelectric material that is integrated into the horns is pre-stressed with flexures. Piezoelectric actuators are attractive for their ability to generate precision high strokes, torques, and forces while operating under relatively harsh conditions (temperatures at single-digit K to as high as 1,273 K). Electromagnetic motors (EM) typically have high rotational speed and low torque. In order to produce a useful torque, these motors are geared down to reduce the speed and increase the torque. This gearing adds mass and reduces the efficiency of the EM. Piezoelectric motors can be designed with high torques and lower speeds directly without the need for gears. Designs were developed for producing rotary motion based on the Barth concept of an ultrasonic horn driving a rotor. This idea was extended to a linear motor design by having the horns drive a slider. The unique feature of these motors is that they can be designed in a monolithic planar structure. The design is a unidirectional motor, which is driven by eight horn actuators, that rotates in the clockwise direction. There are two sets of flexures. The flexures around the piezoelectric material are pre-stress flexures and they pre-load the piezoelectric disks to maintain their being operated under compression when electric field is applied. The other set of flexures is a mounting flexure that attaches to the horn at the nodal point and can be designed to generate a normal force between the horn tip and the rotor so that to first order it operates independently and compensates for the wear between the horn and the rotor.

  19. Evolutionary design of corrugated horn antennas

    NASA Technical Reports Server (NTRS)

    Hoorfar, F.; Manshadi, V.; Jamnejad, A.

    2002-01-01

    An evolutionary progranirnitzg (EP) algorithm is used to optimize pattern of a corrugated circularhorn subject to various constraints on return loss and antenna beamwidth and pattern circularity and low crosspolarization. The EP algorithm uses a Gaussian mutation operator. Examples on design synthesis of a 45 section corrugated horn, with a total of 90 optimization parameters, are presented. The results show excellent and efficient optimization of the desired horn parameters.

  20. Evolutionary design of corrugated horn antennas

    NASA Technical Reports Server (NTRS)

    Hoorfar, F.; Manshadi, V.; Jamnejad, A.

    2002-01-01

    An evolutionary progranirnitzg (EP) algorithm is used to optimize pattern of a corrugated circularhorn subject to various constraints on return loss and antenna beamwidth and pattern circularity and low crosspolarization. The EP algorithm uses a Gaussian mutation operator. Examples on design synthesis of a 45 section corrugated horn, with a total of 90 optimization parameters, are presented. The results show excellent and efficient optimization of the desired horn parameters.

  1. Biological Characterization of Rev Variation in Equine Infectious Anemia Virus

    PubMed Central

    Belshan, Michael; Harris, Matthew E.; Shoemaker, Anne E.; Hope, Thomas J.; Carpenter, Susan

    1998-01-01

    Sequence analysis identified significant variation in the second exon of equine infectious anemia virus (EIAV) rev. Functional analysis indicated that limited amino acid variation in Rev significantly altered the export activity of the protein but did not affect Rev-dependent alternative splicing. EIAV Rev can mediate export through two independent cis-acting Rev-responsive elements (RREs), and differences among Rev variants were more pronounced when both RREs were present. Variation in Rev may be an important mechanism for regulation of virus replication in vivo and may contribute to changes in clinical disease. PMID:9557734

  2. Characterization of bovine immunodeficiency virus rev cDNAs and identification and subcellular localization of the Rev protein.

    PubMed Central

    Oberste, M S; Williamson, J C; Greenwood, J D; Nagashima, K; Copeland, T D; Gonda, M A

    1993-01-01

    One of the six putative accessory genes of bovine immunodeficiency virus (BIV) is similar to those identified as rev in the human immunodeficiency virus and visna virus genomes. To further analyze the BIV rev gene locus, protein, and function, rev cDNAs were cloned and characterized. BIV rev mRNA is derived from the full-length transcript by multiple splicing events and consists of three exons, including the untranslated leader sequence and two coding exons. BIV rev cDNA was expressed in bacteria and in a mammalian in vitro translation expression system. A 23-kDa Rev protein (p23rev) was immunologically detected in lysates from both systems by using an antiserum made to a synthetic Rev peptide. Recombinant p23rev made in bacteria was purified and used to make a polyvalent antiserum. Antisera to Rev peptide and recombinant p23rev immunoprecipitated p23rev from BIV-infected mammalian cells but not from virions. A mammalian expression vector using the BIV rev cDNA was constructed; p23rev was immunoprecipitated with anti-Rev serum from 32P-labeled lysates of monkey cells transfected with this plasmid, demonstrating that BIV Rev is phosphorylated. Immunofluorescence and immunoelectron microscopy with anti-BIV Rev antisera localized Rev in the nucleus and, particularly, in the nucleoli of BIV-infected cells. In functional studies, the expression of BIV Rev was shown to positively regulate the appearance both of Gag protein, which is translated from the unspliced primary viral transcript, and of singly spliced env mRNA but not that of the multiply spliced tat mRNA. These results demonstrate that BIV Rev activity correlates with the known function of lentivirus Rev proteins. Images PMID:8411341

  3. Alongshore Variability of Nearshore Morphology during Net Offshore Bar Migration

    NASA Astrophysics Data System (ADS)

    Price, T.; Dionísio António, S.; Van Kuik, N.; De Wit, L.; Ruessink, G.

    2016-12-01

    On spatial scales from tens of metres to a few kilometres, wave-dominated sandy coasts often exhibit morphological variations with a striking alongshore rhythmicity. A regular alongshore variation of shallower (more landward) and deeper (more seaward) sandbar crests, known as horns and bays, respectively, may drive the patterning of onshore morphology, such as alongshore variations in beach width and dune development. It remains unclear, however, how sandbar morphology affects the beach-dune system on the timescale of months to years, which is often characterised by a cyclic net offshore directed migration of the bar. We used a 15-year data set of planview time-exposure images and the corresponding offshore hydrodynamic data from Egmond aan Zee, The Netherlands, to investigate the coupled development of bar, beach and dune variability during an entire net offshore migration (NOM) cycle. Our observations show a decrease in temporal variation of alongshore variability in sandbar morphology when a bar becomes the outer bar during the NOM-cycle. Whereas the inner-bar morphology developed more freely, the alongshore positions of the outer-bar horns remained relatively fixed. Moreover, the video data reveal that these outer-bar horns regularly shed a so-called Shoreward Propagating Accretionary Wave (SPAW). Accordingly, these SPAWs regularly brought a volume of sand to the same positions along the beach, locally increasing beach width and corresponding to locations of embryo dune abundance. On the long term, the NOM-cycle thus introduces a spatiotemporal periodicity in the development of alongshore variable morphology across the entire nearshore zone.

  4. The rams horn in western history

    NASA Astrophysics Data System (ADS)

    Lubman, David

    2003-10-01

    The shofar or rams horn-one of the most ancient of surviving aerophones-may have originated with early Neolithic herders. The shofar is mentioned frequently and importantly in the Hebrew bible and in later biblical and post-biblical literature. Despite its long history, contemporary ritual uses, and profound symbolic significance to western religion, no documentation of shofar acoustical properties was found. Since ancient times, shepherds of many cultures have fashioned sound instruments from the horns of herd animals for practical and musical uses. Shepherd horns of other cultures exhibit an evolution of form and technology (e.g., the inclusion of finger holes). The shofar is unique in having retained its primitive form. It is suggested that after centuries of practical use, the shofar became emblematic of the shepherd culture. Ritual use then developed, which froze its form. A modern ritual rams horn played by an experienced blower was examined. This rather short horn was determined to have a source strength of 92 dB (A) at 1 m, a fundamental frequency near 420 Hz, and maximum power output between 1.2 and 1.8 kHz. Sample sounds and detection range estimates are provided.

  5. Primary productivity in the Golden Horn.

    PubMed

    Gönüllü, M Talha; Avşar, Yaşar; Bayhan, Hürrem; Sakar, Süeyman; Arslankaya, Ertan; Apaydin, Omer; Kurt, Uĝur

    2005-10-01

    The shores of the Golden Horn--once most important seaport of the region--represented throughout history a romantic and recreational venue. This tributary to the Bosphorus, however, became seriously polluted with the extensive industrialization and rapid population growth in Istanbul over the past century. Two main tributaries, the Alibeykoy and the Kagithane, dumped both liquid and solid waste from residential areas and industry (small and large-scale) into the Golden Horn. As a result of this pollution, the landward three to four kilometers of the estuary became swamped with sediment. The dominance of anaerobic activity resulted in a highly unpleasant smell, and the shallow depth as one progressed up the bay restricted navigation. In early 1997 The Istanbul Metropolitan Municipality began a dredging operation and gradually diverted all domestic and industrial wastewater discharge from the Golden Horn. Since then there have been remarkable improvements in water quality. This paper presents the state of eutrophication through the water body of the Golden Horn; parameters such as DO, TKN, NH(3)-N, NO(3)-N, the total phosphorus (TP) and dissolved phosphorus (PO(4)-P), phytoplankton and chlorophyll-a have been were analyzed in samples of water taken from various points in the Golden Horn. The presence of DO and the phytoplankton, both indicators of primary productivity in an aquatic body, has been evaluated in relation to former conditions.

  6. Swelling of cattle heel horn by urine.

    PubMed

    Gregory, N G

    2004-03-01

    To understand the likely mechanisms responsible for heel horn softening when cattle stand in their own effluent. To examine the effectiveness of some footbath chemicals in preventing heel horn softening. Shavings from the heels of cattle were used in a laboratory test to examine the swelling effects of cow urine, urea, sulphide and ammonia. Formalin, glutaraldehyde, glyoxal, zinc sulphate, copper sulphate, alum, tannic acid and a compound containing nitrocellulose plus nylon, were compared for their ability to prevent swelling in heel shavings induced by urea plus 2-mercaptoethanol. Cow urine caused heel horn to swell. Urea caused swelling at concentrations normally found in cow urine. Sulphide caused swelling at concentrations normally found in cow slurry. Low concentrations of ammonia solution also resulted in swelling. Formalin and glutaraldehyde prevented swelling induced by high concentrations of urea plus 2-mercaptoethanol. Copper sulphate had a moderate anti-swelling effect. Zinc sulphate, alum, tannic acid, and nitrocellulose plus nylon were relatively ineffective in preventing swelling. Cow urine can cause degradation of heel horn in cattle feet. The chemical agents that cause this could be urea, sulphide anions and ammonia. Degradation by urine can be prevented by pretreating heel horn with formalin. Glutaraldehyde may be an effective noncarcinogenic alternative to formalin.

  7. Monolithic Flexure Pre-Stressed Ultrasonic Horns

    NASA Technical Reports Server (NTRS)

    Sherrit, Stewart (Inventor); Bao, Xiaoqi (Inventor); Badescu, Mircea (Inventor); Bar-Cohen, Yoseph (Inventor); Allen, Phillip Grant (Inventor)

    2015-01-01

    A monolithic ultrasonic horn where the horn, backing, and pre-stress structures are combined in a single monolithic piece is disclosed. Pre-stress is applied by external flexure structures. The provision of the external flexures has numerous advantages including the elimination of the need for a pre-stress bolt. The removal of the pre-stress bolt eliminates potential internal electric discharge points in the actuator. In addition, it reduces the chances of mechanical failure in the actuator stacks that result from the free surface in the hole of conventional ring stacks. In addition, the removal of the stress bolt and the corresponding reduction in the overall number of parts reduces the overall complexity of the resulting ultrasonic horn actuator and simplifies the ease of the design, fabrication and integration of the actuator of the present invention into other structures.

  8. Little Big Horn River Water Quality Project

    SciTech Connect

    Bad Bear, D.J.; Hooker, D.

    1995-10-01

    This report summarizes the accomplishments of the Water Quality Project on the Little Big horn River during the summer of 1995. The majority of the summer was spent collecting data on the Little Big Horn River, then testing the water samples for a number of different tests which was done at the Little Big Horn College in Crow Agency, Montana. The intention of this study is to preform stream quality analysis to gain an understanding of the quality of selected portion of the river, to assess any impact that the existing developments may be causing to the environment and to gather base-line data which will serve to provide information concerning the proposed development. Citizens of the reservation have expressed a concern of the quality of the water on the reservation; surface waters, ground water, and well waters.

  9. Dorsal Horn Circuits for Persistent Mechanical Pain.

    PubMed

    Peirs, Cedric; Williams, Sean-Paul G; Zhao, Xinyi; Walsh, Claire E; Gedeon, Jeremy Y; Cagle, Natalie E; Goldring, Adam C; Hioki, Hiroyuki; Liu, Zheng; Marell, Paulina S; Seal, Rebecca P

    2015-08-19

    Persistent mechanical hypersensitivity that occurs in the setting of injury or disease remains a major clinical problem largely because the underlying neural circuitry is still not known. Here we report the functional identification of key components of the elusive dorsal horn circuit for mechanical allodynia. We show that the transient expression of VGLUT3 by a discrete population of neurons in the deep dorsal horn is required for mechanical pain and that activation of the cells in the adult conveys mechanical hypersensitivity. The cells, which receive direct low threshold input, point to a novel location for circuit initiation. Subsequent analysis of c-Fos reveals the circuit extends dorsally to nociceptive lamina I projection neurons, and includes lamina II calretinin neurons, which we show also convey mechanical allodynia. Lastly, using inflammatory and neuropathic pain models, we show that multiple microcircuits in the dorsal horn encode this form of pain.

  10. Monolithic Flexure Pre-Stressed Ultrasonic Horns

    NASA Technical Reports Server (NTRS)

    Sherrit, Stewart (Inventor); Bao, Xiaoqi (Inventor); Badescu, Mircea (Inventor); Bar-Cohen, Yoseph (Inventor); Allen, Phillip Grant (Inventor)

    2016-01-01

    A monolithic ultrasonic horn where the horn, backing, and pre-stress structures are combined in a single monolithic piece is disclosed. Pre-stress is applied by external flexure structures. The provision of the external flexures has numerous advantages including the elimination of the need for a pre-stress bolt. The removal of the pre-stress bolt eliminates potential internal electric discharge points in the actuator. In addition, it reduces the chances of mechanical failure in the actuator stacks that result from the free surface in the hole of conventional ring stacks. In addition, the removal of the stress bolt and the corresponding reduction in the overall number of parts reduces the overall complexity of the resulting ultrasonic horn actuator and simplifies the ease of the design, fabrication and integration of the actuator of the present invention into other structures.

  11. The Bovine Immunodeficiency Virus Rev Protein: Identification of a Novel Nuclear Import Pathway and Nuclear Export Signal among Retroviral Rev/Rev-Like Proteins

    PubMed Central

    Gomez Corredor, Andrea

    2012-01-01

    The Rev protein is essential for the replication of lentiviruses. Rev is a shuttling protein that transports unspliced and partially spliced lentiviral RNAs from the nucleus to the cytoplasm via the nucleopore. To transport these RNAs, the human immunodeficiency virus type 1 (HIV-1) Rev uses the karyopherin β family importin β and CRM1 proteins that interact with the Rev nuclear localization signal (NLS) and nuclear exportation signal (NES), respectively. Recently, we reported the presence of new types of bipartite NLS and nucleolar localization signal (NoLS) in the bovine immunodeficiency virus (BIV) Rev protein. Here we report the characterization of the nuclear import and export pathways of BIV Rev. By using an in vitro nuclear import assay, we showed that BIV Rev is transported into the nucleus by a cytosolic and energy-dependent importin α/β classical pathway. Results from glutathione S-transferase (GST) pulldown assays that showed the binding of BIV Rev with importins α3 and α5 were in agreement with those from the nuclear import assay. We also identified a leptomycin B-sensitive NES in BIV Rev, which indicates that the protein is exported via CRM1 like HIV-1 Rev. Mutagenesis experiments showed that the BIV Rev NES maps between amino acids 109 to 121 of the protein. Remarkably, the BIV Rev NES was found to be of the cyclic AMP (cAMP)-dependent protein kinase inhibitor (PKI) type instead of the HIV-1 Rev type. In summary, our data showed that the nuclear import mechanism of BIV Rev is novel among Rev proteins characterized so far in lentiviruses. PMID:22379104

  12. 75 FR 71069 - Big Horn County Resource Advisory Committee

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-11-22

    ... Forest Service Big Horn County Resource Advisory Committee AGENCY: Forest Service, USDA. ACTION: Notice of meeting. SUMMARY: The Big Horn County Resource Advisory Committee will meet in Greybull, Wyoming... December 1, 2010, and will begin at 10 a.m. ADDRESSES: The meeting will be held at the Big Horn County...

  13. 76 FR 7810 - Big Horn County Resource Advisory Committee

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-02-11

    ... Forest Service Big Horn County Resource Advisory Committee AGENCY: Forest Service, USDA. ACTION: Notice of meeting. SUMMARY: The Big Horn County Resource Advisory Committee will meet in Lovell, Wyoming..., 2011, and will begin at 10 a.m. ADDRESSES: The meeting will be held at the Big Horn Federal Savings...

  14. 76 FR 26240 - Big Horn County Resource Advisory Committee

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-05-06

    ... Forest Service Big Horn County Resource Advisory Committee AGENCY: Forest Service, USDA. ACTION: Notice of meeting. SUMMARY: The Big Horn County Resource Advisory Committee will meet in Greybull, Wyoming..., 2011, and will begin at 10 a.m. ADDRESSES: The meeting will be held at the Big Horn County Weed and...

  15. Offshore Wind Energy

    SciTech Connect

    Strach-Sonsalla, Mareike; Stammler, Matthias; Wenske, Jan; Jonkman, Jason; Vorpahl, Fabian

    2016-07-27

    In 1991, the Vindeby Offshore Wind Farm, the first offshore wind farm in the world, started feeding electricity to the grid off the coast of Lolland, Denmark. Since then, offshore wind energy has developed from this early experiment to a multibillion dollar market and an important pillar of worldwide renewable energy production. Unit sizes grew from 450 kW at Vindeby to the 7.5 MW-class offshore wind turbines (OWT ) that are currently (by October 2014) in the prototyping phase. This chapter gives an overview of the state of the art in offshore wind turbine (OWT) technology and introduces the principles of modeling and simulating an OWT. The OWT components -- including the rotor, nacelle, support structure, control system, and power electronics -- are introduced, and current technological challenges are presented. The OWT system dynamics and the environment (wind and ocean waves) are described from the perspective of OWT modelers and designers. Finally, an outlook on future technology is provided. The descriptions in this chapter are focused on a single OWT -- more precisely, a horizontal-axis wind turbine -- as a dynamic system. Offshore wind farms and wind farm effects are not described in detail in this chapter, but an introduction and further references are given.

  16. The National Implications of "Cain v. Horne"

    ERIC Educational Resources Information Center

    Keller, Tim

    2009-01-01

    This commentary addresses the Arizona Supreme Court's legal reasoning in "Cain v. Horne," which struck down two voucher programs for special needs children pursuant to one of Arizona's Blaine Amendments and explains that the court both failed to apply a straightforward textual analysis and ignored the analytical framework its prior…

  17. 49 CFR 229.129 - Locomotive horn.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... produces a minimum sound level of 96 dB(A) and a maximum sound level of 110 dB(A) at 100 feet forward of...), or (b)(3) of this section, shall not be required to undergo sound level testing when equipped with a... locomotive horn sound level shall be in accordance with the following requirements: (1) A properly...

  18. 49 CFR 229.129 - Locomotive horn.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... produces a minimum sound level of 96 dB(A) and a maximum sound level of 110 dB(A) at 100 feet forward of...), or (b)(3) of this section, shall not be required to undergo sound level testing when equipped with a... locomotive horn sound level shall be in accordance with the following requirements: (1) A properly...

  19. 49 CFR 229.129 - Locomotive horn.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... produces a minimum sound level of 96 dB(A) and a maximum sound level of 110 dB(A) at 100 feet forward of...), or (b)(3) of this section, shall not be required to undergo sound level testing when equipped with a... locomotive horn sound level shall be in accordance with the following requirements: (1) A properly...

  20. Reflectance measurement validation using acoustic horns.

    PubMed

    Rasetshwane, Daniel M; Neely, Stephen T

    2015-10-01

    Variability in wideband acoustic reflectance (and absorbance) measurements adversely affects the clinical utility of reflectance for diagnosis of middle-ear disorders. A reflectance standard would encourage consistency across different measurement systems and help identify calibration related issues. Theoretical equations exist for the reflectance of finite-length exponential, conical, and parabolic acoustic horns. Reflectance measurements were repeatedly made in each of these three horn shapes and the results were compared to the corresponding theoretical reflectance. A method is described of adjusting acoustic impedance measurements to compensate for spreading of the wave front that propagates from the small diameter sound port of the probe to the larger diameter of the acoustic cavity. Agreement between measured and theoretical reflectance was less than 1 dB at most frequencies in the range from 0.2 to 10 kHz. Pearson correlation coefficients were greater than 0.95 between measured and theoretical time-domain reflectance within the flare region of the horns. The agreement suggests that the distributed reflectance of acoustic horns may be useful for validating reflectance measurements made in human ear canals; however, refinements to reflectance measurement methods may still be needed.

  1. The BigHorn Home Improvement Center

    SciTech Connect

    2000-12-01

    The BigHorn Home Improvement Center is one of the nation's first commercial buildings to integrate daylighting and natural ventilation cooling systems into a retail space. It is expected to reduce energy costs by 62% compared to conventionally designed retail buildings.

  2. Reflectance measurement validation using acoustic horns

    PubMed Central

    Rasetshwane, Daniel M.; Neely, Stephen T.

    2015-01-01

    Variability in wideband acoustic reflectance (and absorbance) measurements adversely affects the clinical utility of reflectance for diagnosis of middle-ear disorders. A reflectance standard would encourage consistency across different measurement systems and help identify calibration related issues. Theoretical equations exist for the reflectance of finite-length exponential, conical, and parabolic acoustic horns. Reflectance measurements were repeatedly made in each of these three horn shapes and the results were compared to the corresponding theoretical reflectance. A method is described of adjusting acoustic impedance measurements to compensate for spreading of the wave front that propagates from the small diameter sound port of the probe to the larger diameter of the acoustic cavity. Agreement between measured and theoretical reflectance was less than 1 dB at most frequencies in the range from 0.2 to 10 kHz. Pearson correlation coefficients were greater than 0.95 between measured and theoretical time-domain reflectance within the flare region of the horns. The agreement suggests that the distributed reflectance of acoustic horns may be useful for validating reflectance measurements made in human ear canals; however, refinements to reflectance measurement methods may still be needed. PMID:26520306

  3. The National Implications of "Cain v. Horne"

    ERIC Educational Resources Information Center

    Keller, Tim

    2009-01-01

    This commentary addresses the Arizona Supreme Court's legal reasoning in "Cain v. Horne," which struck down two voucher programs for special needs children pursuant to one of Arizona's Blaine Amendments and explains that the court both failed to apply a straightforward textual analysis and ignored the analytical framework its prior…

  4. Monolithic Flexure Pre-Stressed Ultrasonic Horns

    NASA Technical Reports Server (NTRS)

    Sherrit, Stewart; Bao, Xiaoqi; Badescu, Mircea; Bar-Cohen, Yoseph; Allen, Phillip Grant

    2011-01-01

    High-power ultrasonic actuators are generally assembled with a horn, backing, stress bolt, piezoelectric rings, and electrodes. The manufacturing process is complex, expensive, difficult, and time-consuming. The internal stress bolt needs to be insulated and presents a potential internal discharge point, which can decrease actuator life. Also, the introduction of a center hole for the bolt causes many failures, reducing the throughput of the manufactured actuators. A new design has been developed for producing ultrasonic horn actuators. This design consists of using flexures rather than stress bolts, allowing one to apply pre-load to the piezoelectric material. It also allows one to manufacture them from a single material/plate, rapid prototype them, or make an array in a plate or 3D structure. The actuator is easily assembled, and application of pre-stress greater than 25 MPa was demonstrated. The horn consists of external flexures that eliminate the need for the conventional stress bolt internal to the piezoelectric, and reduces the related complexity. The stress bolts are required in existing horns to provide prestress on piezoelectric stacks when driven at high power levels. In addition, the manufacturing process benefits from the amenability to produce horn structures with internal cavities. The removal of the pre-stress bolt removes a potential internal electric discharge point in the actuator. In addition, it significantly reduces the chances of mechanical failure in the piezoelectric stacks that result from the hole surface in conventional piezoelectric actuators. The novel features of this disclosure are: 1. A design that can be manufactured from a single piece of metal using EDM, precision machining, or rapid prototyping. 2. Increased electromechanical coupling of the horn actuator. 3. Higher energy density. 4. A monolithic structure of a horn that consists of an external flexure or flexures that can be used to pre-stress a solid piezoelectric structure

  5. Getting offshoring right.

    PubMed

    Aron, Ravi; Singh, Jitendra V

    2005-12-01

    The prospect of offshoring and outsourcing business processes has captured the imagination of CEOs everywhere. In the past five years, a rising number of companies in North America and Europe have experimented with this strategy, hoping to reduce costs and gain strategic advantage. But many businesses have had mixed results. According to several studies, half the organizations that have shifted processes offshore have failed to generate the expected financial benefits. What's more, many of them have faced employee resistance and consumer dissatisfaction. Clearly, companies have to rethink how they formulate their offshoring strategies. A three-part methodology can help. First, companies need to prioritize their processes, ranking each based on two criteria: the value it creates for customers and the degree to which the company can capture some of that value. Companies will want to keep their core (highest-priority) processes in-house and consider outsourcing their commodity (low-priority) processes; critical (moderate-priority) processes are up for debate and must be considered carefully. Second, businesses should analyze all the risks that accompany offshoring and look systematically at their critical and commodity processes in terms of operational risk (the risk that processes won't operate smoothly after being offshored) and structural risk (the risk that relationships with service providers may not work as expected). Finally, companies should determine possible locations for their offshore efforts, as well as the organizational forms--such as captive centers and joint ventures--that those efforts might take. They can do so by examining each process's operational and structural risks side by side. This article outlines the tools that will help companies choose the right processes to offshore. It also describes a new organizational structure called the extended organization, in which companies specify the quality of services they want and work alongside providers

  6. Mechanical and biological properties of oxidized horn keratin.

    PubMed

    Zhang, Quanbin; Shan, Guanghua; Cao, Ping; He, Jia; Lin, Zhongshi; Huang, Yaoxiong; Ao, Ningjian

    2015-02-01

    The goal of this study was to investigate the mechanical and biological properties of oxidized keratin materials, which were obtained by using buffalo horns to oxidize. It could provide a way to evaluate their potential for clinical translatability. The characterization on their composition, mechanical properties, and biological responses was performed. It is found that the oxidation process could lead the disulfide bond to break down and then to form sulfonic acid, or even make partial peptide chain to be fragment for the new modification of amino acid. Hence the oxidized horn keratins have lower thermal stability and hydrolytic stability in comparison with horn keratin, but the degradation products of oxidized horn keratins have no significant difference. In addition, the mechanical properties of oxidized horn keratins are poorer than that of horn keratin, but the oxidized horn keratins still have disulfide bonds to form a three-dimensional structure, which benefits for their mechanical properties. The fracture toughness of oxidized horn keratins increases with the increase in the degree of oxidation. After oxidation, the oxidized horn keratins have lower cytotoxicity and lower hemolysis ratio. Moreover, when the oxidized horn keratins, as well as different concentration of degradation products of oxidized horn keratins, are directly in contact with platelet-rich plasma, platelets are not activated. It suggests that the oxidized horn keratins have good hemocompatibility, without triggering blood thrombosis. The implantation experiment in vivo also demonstrates that the oxidized horn keratins are compatible with the tissue, because there are minimal fibrous capsule and less of infiltration of host cells, without causing serious inflammation. In summary, the oxidized horn keratins can act as implanted biomaterial devices that are directly in contact with blood and tissue. Copyright © 2014 Elsevier B.V. All rights reserved.

  7. A nucleolar localizing Rev binding element inhibits HIV replication

    PubMed Central

    Michienzi, Alessandro; De Angelis, Fernanda G; Bozzoni, Irene; Rossi, John J

    2006-01-01

    The Rev protein of the human immunodeficiency virus (HIV) facilitates the nuclear export of intron containing viral mRNAs allowing formation of infectious virions. Rev traffics through the nucleolus and shuttles between the nucleus and cytoplasm. Rev multimerization and interaction with the export protein CRM1 takes place in the nucleolus. To test the importance of Rev nucleolar trafficking in the HIV-1 replication cycle, we created a nucleolar localizing Rev Response Element (RRE) decoy and tested this for its anti-HIV activity. The RRE decoy provided marked inhibition of HIV-1 replication in both the CEM T-cell line and in primary CD34+ derived monocytes. These results demonstrate that titration of Rev in the nucleolus impairs HIV-1 replication and supports a functional role for Rev trafficking in this sub-cellular compartment. PMID:16712721

  8. Energy from Offshore Wind: Preprint

    SciTech Connect

    Musial, W.; Butterfield, S.; Ram, B.

    2006-02-01

    This paper provides an overview of the nascent offshore wind energy industry including a status of the commercial offshore industry and the technologies that will be needed for full market development.

  9. Dual band combiner for horn antenna

    NASA Technical Reports Server (NTRS)

    Williams, W. F.; Cohn, S. B. (Inventor)

    1980-01-01

    A corrugated horn antenna, adapted to be coupled to a waveguide at its apex for X-band excitation is further adapted to be connected to waveguides through a circumferential slot for S-band excitation at four distinct phases selected for the desired S-band polarization. The circumferential slot is positioned along the axial length of the horn for good impedance matching and is provided with an X-band choke in the form of two concentric choke slots. For further improvement in impedance matching, the second (outer) choke slot is divided by plugs into four segments that coincide with waveguide ports for the four distinct phases of the S-band.

  10. Lethal arthrogryposis with anterior horn cell disease.

    PubMed

    Vuopala, K; Ignatius, J; Herva, R

    1995-01-01

    Fifteen infants (11 families) with lethal arthrogryposis and anterior horn motor neuron loss are described. The clinical presentation was the fetal akinesia deformation sequence (FADS) with multiple contractures and facial anomalies. At autopsy neurogenic muscular atrophy was present in all infants. The spinal cord showed a paucity of anterior horn motor neurons in the 12 infants studied. Both male and female infants were affected. Nine cases were sporadic, whereas in two families there were three affected cases. Consanguinity between the parents was reported in one family with one affected child. This and the recurrence of the condition speak for autosomal recessive inheritance. Detailed neuropathological examination and documentation of the clinical features are needed for a better delineation of and genetic counseling for perinatally lethal arthrogryposis.

  11. Rev-Free HIV-1 Gene Delivery System for Targeting Rev-RRE-Crm1 Nucleocytoplasmic RNA Transport Pathway

    PubMed Central

    Srinivasakumar, Narasimhachar

    2011-01-01

    The use of RNA transport elements from different viruses can provide novel attributes to HIV-1-based gene delivery systems such as improved safety or Rev independence. We previously described an HIV-1 based gene delivery system that utilized the simian immunodeficiency virus Rev-response element (RRE) in place of the HIV-1 RRE. Despite the use of Rev for the production of vector stocks, we showed the utility of this system for delivery of Rev M10, a dominant-negative mutant of HIV-1 Rev, into T-cells. Here, we investigated the use of RNA transport elements from Mason-Pfizer monkey virus or MPMV for the creation of high-titered Rev-free HIV-1-based packaging systems. The HIV-1 gag/pol expression constructs containing one or more copies of MPMV constitutive RNA transport element (CTE) were used to package similarly modified gene-transfer vectors in the presence or absence of Rev. An inverse correlation between the number of CTE modules and Rev dependency was noted for vector stock production. While packaging systems containing multiple CTEs were resistant to exogenously expressed Rev M10, the titers of vectors encoding Rev M10 were nevertheless reduced in comparison to vectors encoding only green fluorescent protein (GFP). In contrast, a gene transfer vector encoding the Rev M10 transgene and containing both RNA transport elements exhibited almost no loss in titer in comparison to a corresponding vector encoding only GFP. The optimized Rev-independent gene delivery system was used for delivery of Rev M10 transgene into T-lymphocytes. Upon challenge in single round infection assays with HIV-1, the modified T-cells produced fewer virus particles than control cells expressing GFP. This Rev-free packaging system may prove useful for targeting the Rev-RRE-Crm1 nucleocytoplasmic RNA transport pathway for inhibiting HIV replication. PMID:22164294

  12. Rev-free HIV-1 gene delivery system for targeting Rev-RRE-Crm1 nucleocytoplasmic RNA transport pathway.

    PubMed

    Srinivasakumar, Narasimhachar

    2011-01-01

    The use of RNA transport elements from different viruses can provide novel attributes to HIV-1-based gene delivery systems such as improved safety or Rev independence. We previously described an HIV-1 based gene delivery system that utilized the simian immunodeficiency virus Rev-response element (RRE) in place of the HIV-1 RRE. Despite the use of Rev for the production of vector stocks, we showed the utility of this system for delivery of Rev M10, a dominant-negative mutant of HIV-1 Rev, into T-cells. Here, we investigated the use of RNA transport elements from Mason-Pfizer monkey virus or MPMV for the creation of high-titered Rev-free HIV-1-based packaging systems. The HIV-1 gag/pol expression constructs containing one or more copies of MPMV constitutive RNA transport element (CTE) were used to package similarly modified gene-transfer vectors in the presence or absence of Rev. An inverse correlation between the number of CTE modules and Rev dependency was noted for vector stock production. While packaging systems containing multiple CTEs were resistant to exogenously expressed Rev M10, the titers of vectors encoding Rev M10 were nevertheless reduced in comparison to vectors encoding only green fluorescent protein (GFP). In contrast, a gene transfer vector encoding the Rev M10 transgene and containing both RNA transport elements exhibited almost no loss in titer in comparison to a corresponding vector encoding only GFP. The optimized Rev-independent gene delivery system was used for delivery of Rev M10 transgene into T-lymphocytes. Upon challenge in single round infection assays with HIV-1, the modified T-cells produced fewer virus particles than control cells expressing GFP. This Rev-free packaging system may prove useful for targeting the Rev-RRE-Crm1 nucleocytoplasmic RNA transport pathway for inhibiting HIV replication.

  13. Offshore rectenna feasbility

    NASA Technical Reports Server (NTRS)

    Freeman, J. W.; Hervey, D.; Glaser, P.

    1980-01-01

    A preliminary study of the feasibility and cost of an offshore rectenna to serve the upper metropolitan east coast was performed. A candidate site at which to build a 5 GW rectenna was selected on the basis of proximity to load centers, avoidance of shipping lanes, sea floor terrain, and relocated conditions. Several types of support structures were selected for study based initially on the reference system rectenna concept of a wire mesh ground screen and dipoles each with its own rectifier and filter circuits. Possible secondary uses of an offshore rectenna were examined and are evaluated.

  14. Great horned owls are released at CCAFS

    NASA Technical Reports Server (NTRS)

    2000-01-01

    Susan Small, director of the Florida Wildlife Hospital, holds a great horned owl before releasing it at Cape Canaveral Air Force Station, Complex 25/29. The owl is one of two found in June on the floor of CCAFS Hangar G, where their nest was located. They were treated at a local veterinary hospital and then taken to the Florida Wildlife Hospital in Melbourne for care and rehabilitation before release.

  15. Great horned owls are released at CCAFS

    NASA Technical Reports Server (NTRS)

    2000-01-01

    A great horned owl flies to freedom after its release at Cape Canaveral Air Force Station, Complex 25/29. The owl is one of two found in June on the floor of CCAFS Hangar G, where their nest was located. They were treated at a local veterinary hospital and then taken to the Florida Wildlife Hospital in Melbourne for care and rehabilitation before release.

  16. Great horned owls are released at CCAFS

    NASA Technical Reports Server (NTRS)

    2000-01-01

    Eileen Olejarski (left), manager of Florida Wildlife Hospital, holds a great horned owl before releasing it at Cape Canaveral Air Force Station, Complex 25/29. The owl is one of two found in June on the floor of CCAFS Hangar G, where their nest was located. They were treated at a local veterinary hospital and then taken to the Florida Wildlife Hospital in Melbourne for care and rehabilitation before release.

  17. Great horned owls are released at CCAFS

    NASA Technical Reports Server (NTRS)

    2000-01-01

    Eileen Olejarski (left), manager of Florida Wildlife Hospital, and Susan Small, director of the hospital, get ready to release two great horned owls at Cape Canaveral Air Force Station, Complex 25/29. The owls were found in June on the floor of CCAFS Hangar G, where their nest was located. They were treated at a local veterinary hospital and then taken to the Florida Wildlife Hospital in Melbourne for care and rehabilitation before release.

  18. Great horned owls are released at CCAFS

    NASA Technical Reports Server (NTRS)

    2000-01-01

    Eileen Olejarski (left), manager of Florida Wildlife Hospital, and Susan Small, director of the hospital, remove two great horned owls from the vehicle before releasing them at Cape Canaveral Air Force Station, Complex 25/29. The owls were found in June on the floor of CCAFS Hangar G, where their nest was located. They were treated at a local veterinary hospital and then taken to the Florida Wildlife Hospital in Melbourne for care and rehabilitation before release..

  19. Terahertz Horn Antennas on Thin Membranes

    DTIC Science & Technology

    1992-05-01

    Stimson. David B. Rutledge, "Monolithic Millimeter -Wave Two-Dimensional Horn Imaging Arrays.-’ IEEE Transactions on Antennas and Propagation, T-AP-38...Density Meter for Millimeter Vavelengths." IEEE Transactions on Antennas and Propagation, T-AP-39, pp. 425-428, March 1991. [3]Yong Guo, Karen Lee...calculated patterns. Microbolometers have been used as detectors, as they can be calibrated to yield abso- lute power measurements. This is important

  20. Great horned owls are released at CCAFS

    NASA Technical Reports Server (NTRS)

    2000-01-01

    A great horned owl flies to freedom after its release at Cape Canaveral Air Force Station, Complex 25/29. The owl is one of two found in June on the floor of CCAFS Hangar G, where their nest was located. They were treated at a local veterinary hospital and then taken to the Florida Wildlife Hospital in Melbourne for care and rehabilitation before release.

  1. Great horned owls are released at CCAFS

    NASA Technical Reports Server (NTRS)

    2000-01-01

    Eileen Olejarski (left), manager of Florida Wildlife Hospital, and Susan Small, director of the hospital, get ready to release two great horned owls at Cape Canaveral Air Force Station, Complex 25/29. The owls were found in June on the floor of CCAFS Hangar G, where their nest was located. They were treated at a local veterinary hospital and then taken to the Florida Wildlife Hospital in Melbourne for care and rehabilitation before release.

  2. Great horned owls are released at CCAFS

    NASA Technical Reports Server (NTRS)

    2000-01-01

    Eileen Olejarski (left), manager of Florida Wildlife Hospital, and Susan Small, director of the hospital, remove two great horned owls from the vehicle before releasing them at Cape Canaveral Air Force Station, Complex 25/29. The owls were found in June on the floor of CCAFS Hangar G, where their nest was located. They were treated at a local veterinary hospital and then taken to the Florida Wildlife Hospital in Melbourne for care and rehabilitation before release..

  3. Great horned owls are released at CCAFS

    NASA Technical Reports Server (NTRS)

    2000-01-01

    Eileen Olejarski (left), manager of Florida Wildlife Hospital, holds a great horned owl before releasing it at Cape Canaveral Air Force Station, Complex 25/29. The owl is one of two found in June on the floor of CCAFS Hangar G, where their nest was located. They were treated at a local veterinary hospital and then taken to the Florida Wildlife Hospital in Melbourne for care and rehabilitation before release.

  4. Great horned owls are released at CCAFS

    NASA Technical Reports Server (NTRS)

    2000-01-01

    Susan Small, director of the Florida Wildlife Hospital, holds a great horned owl before releasing it at Cape Canaveral Air Force Station, Complex 25/29. The owl is one of two found in June on the floor of CCAFS Hangar G, where their nest was located. They were treated at a local veterinary hospital and then taken to the Florida Wildlife Hospital in Melbourne for care and rehabilitation before release.

  5. Acoustical Analysis of a Test Horn.

    DTIC Science & Technology

    1988-01-20

    computer program COMIC (COMplex AcoustIC Pressure Analysis). The mode shapes are presented graphically and nodal -panes are identified. Placing a wall of...shapes of a small test horn using a computer program COMIC (fO.plex AcoustIC Pressure Analysis), which was developed by Battelle Columbus...written to convert the COMIC output data into the one which operates on CADS (Computer Aided Design System) using DI-3000 graphics package. Reference

  6. Countering Piracy off the Horn of Africa

    DTIC Science & Technology

    2011-01-26

    6 Source: Conciliation Resources.14 Piracy operations at the Horn of Africa are carried out using a method unique compared to piracy...option would continue to treat the system with the same medicine , but increase the dose. This may include strengthening the task forces to allow...visualization.googlemap&I temid=219 (accessed January 18, 2011). 14 Conciliation Resources Home Page, Map of Somalia, http://www.c-r.org/our- work

  7. Molecular identification of antelope horn by melting curve analysis.

    PubMed

    Tong, Yuru; Jiang, Chao; Yuan, Yuan; Jin, Yan; Cui, Zhan-Hu; Huang, Luqi

    2016-11-01

    Antelope horn is a valuable Chinese traditional medicine and widely used in clinic. However, with the deterioration of antelope's living environment and a lot of killing, the saiga population begins falling and in some places plummet. Since the increasing demand of this expensive and good bioactive medicine, the horn of artiodactyla animals is often used as the antelope horn. The adulterated or impostor not only cause damage to clinical medicine but also affect the antelope resources protection and sustainable development. Here, in order to establish a melting curve analysis (MCA) method to distinguish the antelope horn from other animal horns and identify the decoction pieces and Chinese patent medicine in a fast and easy way, animal horns and its decoction pieces, Chinese patent medicines were collected from the market and the DNA of all the collected samples were extracted. The melting curve of two universal fragments (COI and Cyt b) was scanned and Cyt b was selected as feasibility fragment for identifying authentic antelope horn from eight adulterant animal horns. After optimizing the condition for MCA, inspecting the precision and the replication of the method, a reference melting curve modern was established and we performed MCA on the antelope horns, fakes, and adulterants on a 1:1 mix, decoction pieces, and Chinese patent medicine. Thus, this study provides fast and easy methods so that MCA can detect the truth, fakes, and adulterations of antelope horns.

  8. Offshore platform cathodic protection retrofits

    SciTech Connect

    Turnipseed, S.P.

    1996-10-01

    Cathodic protection (CP) is the primary technique used for underwater corrosion control on the majority of offshore steel structures. Offshore platforms are often kept in service far beyond their original design life. Refurbishment of the CP system is required when adequate protection can no longer be maintained. Various offshore platform CP retrofit designs are discussed.

  9. Beetle horns and horned beetles: emerging models in developmental evolution and ecology

    PubMed Central

    Kijimoto, Teiya; Pespeni, Melissa; Beckers, Oliver; Moczek, Armin P.

    2013-01-01

    Many important questions in developmental biology increasingly interface with related questions in other biological disciplines such as evolutionary biology and ecology. In this article, we review and summarize recent progress in the development of horned beetles and beetle horns as study systems amenable to the integration of a wide range of approaches, from gene function analysis in the laboratory to population ecological and behavioral studies in the field. Specifically, we focus on three key questions at the current interface of developmental biology, evolutionary biology and ecology: (1) the developmental mechanisms underlying the origin and diversification of novel, complex traits, (2) the relationship between phenotypic diversification and the diversification of genes and transcriptomes, and (3) the role of behavior as a leader or follower in developmental evolution. For each question we discuss how work on horned beetles is contributing to our current understanding of key issues, as well as highlight challenges and opportunities for future studies. PMID:23799584

  10. Offshore Wind Energy Systems

    ERIC Educational Resources Information Center

    Musgrove, P.

    1978-01-01

    Explores the possibility of installing offshore windmills to provide electricity and to save fuel for the United Kingdom. Favors their deployment in clusters to facilitate supervision and minimize cost. Discusses the power output and the cost involved and urges their quick development. (GA)

  11. The state of offshore

    SciTech Connect

    Nelson, B.F.

    1991-01-01

    In this book, the author explains the factors behind state involvement in offshore petroleum activities. From his analysis of government workings in Great Britain and Norway, he concludes that state intervention is determined by complex interactions among government officials, economic interests, and environmental pressures.

  12. Suppression of atherosclerosis by synthetic REV-ERB agonist

    SciTech Connect

    Sitaula, Sadichha; Billon, Cyrielle; Kamenecka, Theodore M.; Solt, Laura A.; Burris, Thomas P.

    2015-05-08

    The nuclear receptors for heme, REV-ERBα and REV-ERBβ, play important roles in the regulation of metabolism and inflammation. Recently it was demonstrated that reduced REV-ERBα expression in hematopoetic cells in LDL receptor null mice led to increased atherosclerosis. We sought to determine if synthetic REV-ERB agonists that we have developed might have the ability to suppress atherosclerosis in this model. A previously characterized synthetic REV-ERB agonist, SR9009, was used to determine if activation of REV-ERB activity would affect atherosclerosis in LDL receptor deficient mice. Atherosclerotic plaque size was significantly reduced (p < 0.05) in mice administered SR9009 (100 mg/kg) for seven weeks compared to control mice (n = 10 per group). SR9009 treatment of bone marrow-derived mouse macrophages (BMDM) reduced the polarization of BMDMs to proinflammatory M1 macrophage while increasing the polarization of BMDMs to anti-inflammatory M2 macrophages. Our results suggest that pharmacological targeting of REV-ERBs may be a viable therapeutic option for treatment of atherosclerosis. - Highlights: • Synthetic REV-ERB agonist treatment reduced atherosclerosis in a mouse model. • Pharmacological activation of REV-ERB decreased M1 macrophage polarization. • Pharmacological activation of REV-ERB increased M2 macrophage polarization.

  13. Human Rev1 polymerase disrupts G-quadruplex DNA

    PubMed Central

    Eddy, Sarah; Ketkar, Amit; Zafar, Maroof K.; Maddukuri, Leena; Choi, Jeong-Yun; Eoff, Robert L.

    2014-01-01

    The Y-family DNA polymerase Rev1 is required for successful replication of G-quadruplex DNA (G4 DNA) in higher eukaryotes. Here we show that human Rev1 (hRev1) disrupts G4 DNA structures and prevents refolding in vitro. Nucleotidyl transfer by hRev1 is not necessary for mechanical unfolding to occur. hRev1 binds G4 DNA substrates with Kd,DNA values that are 4–15-fold lower than those of non-G4 DNA substrates. The pre-steady-state rate constant of deoxycytidine monophosphate (dCMP) insertion opposite the first tetrad-guanine by hRev1 is ∼56% as fast as that observed for non-G4 DNA substrates. Thus, hRev1 can promote fork progression by either dislodging tetrad guanines to unfold the G4 DNA, which could assist in extension by other DNA polymerases, or hRev1 can prevent refolding of G4 DNA structures. The hRev1 mechanism of action against G-quadruplexes helps explain why replication progress is impeded at G4 DNA sites in Rev1-deficient cells and illustrates another unique feature of this enzyme with important implications for genome maintenance. PMID:24366879

  14. Offshore Space Center (offshore launch site)

    NASA Technical Reports Server (NTRS)

    Harvey, D. G.

    1980-01-01

    Any activity requiring the development of the HLLV can benefit by operations from an offshore space center (OSC) since operating near the equator provides a twenty percent increase in payload in an ecliptic plan orbit. Some OSC concepts considered include a moored floating (semisubmersible) design, a stationary design supported by fixed piles, and a combination of these two. The facility supports: a 15,000 foot long, 300 foot wide runway, designed to accommodate a two staged winged launch vehicle, with a one million pound payload capacity to low earth orbit; an industrial area for HLLV maintenance; an airport terminal, control and operation center, and observation tower; liquid hydrogen and liquid oxygen production and storage, and fuel storage platforms; a power generation station, docks with an unloading area; two separate launch sites; and living accommodations for 10,000 people. Potential sites include the Paramount Seamount in the Pacific Ocean off the north coast of South America. Cost estimates are considered.

  15. Offshore Wind Research (Fact Sheet)

    SciTech Connect

    Not Available

    2011-10-01

    This 2-page fact sheet describes NREL's offshore wind research and development efforts and capabilities. The National Renewable Energy Laboratory is internationally recognized for offshore wind energy research and development (R&D). Its experience and capabilities cover a wide spectrum of wind energy disciplines. NREL's offshore wind R&D efforts focus on critical areas that address the long-term needs of the offshore wind energy industry and the Department of Energy (DOE). R&D efforts include: (1) Developing offshore design tools and methods; (2) Collaborating with international partners; (3) Testing offshore systems and developing standards; (4) Conducting economic analyses; (5) Characterizing offshore wind resources; and (6) Identifying and mitigating offshore wind grid integration challenges and barriers. NREL has developed and maintains a robust, open-source, modular computer-aided engineering (CAE) tool, known as FAST. FAST's state-of-the-art capabilities provide full dynamic system simulation for a range of offshore wind systems. It models the coupled aerodynamic, hydrodynamic, control system, and structural response of offshore wind systems to support the development of innovative wind technologies that are reliable and cost effective. FAST also provides dynamic models of wind turbines on offshore fixed-bottom systems for shallow and transitional depths and floating-platform systems in deep water, thus enabling design innovation and risk reduction and facilitating higher performance designs that will meet DOE's cost of energy, reliability, and deployment objectives.

  16. Aperture efficiency of integrated-circuit horn antennas

    NASA Technical Reports Server (NTRS)

    Guo, Yong; Lee, Karen; Stimson, Philip; Potter, Kent; Rutledge, David

    1991-01-01

    The aperture efficiency of silicon integrated-circuit horn antennas has been improved by optimizing the length of the dipole probes and by coating the entire horn walls with gold. To make these measurements, a new thin-film power-density meter was developed for measuring power density with accuracies better than 5 percent. The measured aperture efficiency improved from 44 percent to 72 percent at 93 GHz. This is sufficient for use in many applications which now use machined waveguide horns.

  17. Intense selective hunting leads to artificial evolution in horn size.

    PubMed

    Pigeon, Gabriel; Festa-Bianchet, Marco; Coltman, David W; Pelletier, Fanie

    2016-04-01

    The potential for selective harvests to induce rapid evolutionary change is an important question for conservation and evolutionary biology, with numerous biological, social and economic implications. We analyze 39 years of phenotypic data on horn size in bighorn sheep (Ovis canadensis) subject to intense trophy hunting for 23 years, after which harvests nearly ceased. Our analyses revealed a significant decline in genetic value for horn length of rams, consistent with an evolutionary response to artificial selection on this trait. The probability that the observed change in male horn length was due solely to drift is 9.9%. Female horn length and male horn base, traits genetically correlated to the trait under selection, showed weak declining trends. There was no temporal trend in genetic value for female horn base circumference, a trait not directly targeted by selective hunting and not genetically correlated with male horn length. The decline in genetic value for male horn length stopped, but was not reversed, when hunting pressure was drastically reduced. Our analysis provides support for the contention that selective hunting led to a reduction in horn length through evolutionary change. It also confirms that after artificial selection stops, recovery through natural selection is slow.

  18. Beyond "Cain v. Horne": Comment on Tim Keller's "The National Implications of 'Cain v. Horne'"

    ERIC Educational Resources Information Center

    Harmon, Corinne

    2010-01-01

    This commentary is in response to Tim Keller's article "The National Implications of 'Cain v. Horne.'" In his analysis, Keller asserts that the Arizona Supreme Court wrongfully struck down a voucher program for students with special needs by characterizing the voucher as aid to schools rather than aid to students. In this response to…

  19. Beyond "Cain v. Horne": Comment on Tim Keller's "The National Implications of 'Cain v. Horne'"

    ERIC Educational Resources Information Center

    Harmon, Corinne

    2010-01-01

    This commentary is in response to Tim Keller's article "The National Implications of 'Cain v. Horne.'" In his analysis, Keller asserts that the Arizona Supreme Court wrongfully struck down a voucher program for students with special needs by characterizing the voucher as aid to schools rather than aid to students. In this response to…

  20. Experimental rabies in a great horned owl.

    PubMed

    Jorgenson, R D; Gough, P M; Graham, D L

    1976-07-01

    A great horned owl (Bubo virginianus) was fed the carcass of an experimentally infected rabid skunk. The bird developed antibody titer to rabies, detected by passive haemagglutination, 27 days after oral inoculation by ingestion. The owl suppressed the infection until corticosteroid administration, after which a maximum antibody titer was attained. Evidence of active rabies viral infection was seen by fluorescent antibody staining of oral swabs, corneal impression smears and histologic tissue smears, by suckling mouse inoculation of oral swab washings, and by transmission electron microcopy. No clinical signs of rabies virus infection were observed.

  1. Arctic offshore platform

    SciTech Connect

    Bhula, D.N.

    1984-01-24

    An offshore structure is disclosed for use in drilling and producing wells in arctic regions having a conical shaped lower portion that extends above the surface of the water and a cylindrical upper section. The conical portion is provided with a controlled stiffness outer surface for withstanding the loads produced by ice striking the structure. The stiffness properties of the outer shell and flexible members are designed to distribute the load and avoid high local loads on the inner parts of the structure.

  2. Wind Energy: Offshore Permitting

    DTIC Science & Technology

    2008-05-01

    Technological advancements and tax incentives have driven a global expansion in the development of renewable energy resources. Wind energy , in...particular, is now often cited as the fastest growing commercial energy source in the world. Currently, all U.S. wind energy facilities are based on land...authority to permit and regulate offshore wind energy development within the zones of the oceans under its jurisdiction. The federal government and coastal

  3. Planar Rotary Motor Using Ultrasonic Horns

    NASA Technical Reports Server (NTRS)

    Sherrit, Stewart; Bao, Xiaoqi; Badescu, Mircea; Chang, Zensheu; Geiyer, Daniel; Allen, Phillip; Ostlund, Patrick; Bar-Cohen, Yoseph

    2011-01-01

    One of the first piezoelectric motor designs with significant rotational speeds was outlined by Barth. This device used extensional piezoelectric elements to produce a time varying force at a distance r from the center of a centrally supported disk. These extensional actuators produced micro-steps at a high frequency with the end result being macroscopic rotation of the disk and high torque. The rotation direction is controlled by the choice of the actuators and the direction of the extension about the rotor center. A recent advancement in producing pre-stressed power ultrasonic horns using flexures allows for the development of high torque ultrasonic motors based on the Barth's idea that can be fabricated in a 2D plate or in more complicated 3D structures. In addition to the pre-stress flexures the design also allows for the use of flexures to produce the rotor/horn normal force. The torque can be controlled by the number of actuators in the plane and the amplitude of the normal force. This paper will present analytical and experimental results obtained from testing prototype planar motors.

  4. Planar rotary motor using ultrasonic horns

    NASA Astrophysics Data System (ADS)

    Sherrit, Stewart; Bao, Xiaoqi; Badescu, Mircea; Chang, Zensheu; Geiyer, Daniel; Allen, Phillip; Ostlund, Patrick; Bar-Cohen, Yoseph

    2011-04-01

    One of the first piezoelectric motor designs with significant rotational speeds was outlined by Barth. This device used extensional piezoelectric elements to produce a time varying force at a distance r from the center of a centrally supported disk. These extensional actuators produced micro-steps at a high frequency with the end result being macroscopic rotation of the disk and high torque. The rotation direction is controlled by the choice of the actuators and the direction of the extension about the rotor center. A recent advancement in producing pre-stressed power ultrasonic horns using flexures allows for the development of high torque ultrasonic motors based on the Barth's idea that can be fabricated in a 2D plate or in more complicated 3D structures. In addition to the pre-stress flexures the design also allows for the use of flexures to produce the rotor/horn normal force. The torque can be controlled by the number of actuators in the plane and the amplitude of the normal force. This paper will present analytical and experimental results obtained from testing prototype planar motors.

  5. Planar Rotary Motor Using Ultrasonic Horns

    NASA Technical Reports Server (NTRS)

    Sherrit, Stewart; Bao, Xiaoqi; Badescu, Mircea; Chang, Zensheu; Geiyer, Daniel; Allen, Phillip; Ostlund, Patrick; Bar-Cohen, Yoseph

    2011-01-01

    One of the first piezoelectric motor designs with significant rotational speeds was outlined by Barth. This device used extensional piezoelectric elements to produce a time varying force at a distance r from the center of a centrally supported disk. These extensional actuators produced micro-steps at a high frequency with the end result being macroscopic rotation of the disk and high torque. The rotation direction is controlled by the choice of the actuators and the direction of the extension about the rotor center. A recent advancement in producing pre-stressed power ultrasonic horns using flexures allows for the development of high torque ultrasonic motors based on the Barth's idea that can be fabricated in a 2D plate or in more complicated 3D structures. In addition to the pre-stress flexures the design also allows for the use of flexures to produce the rotor/horn normal force. The torque can be controlled by the number of actuators in the plane and the amplitude of the normal force. This paper will present analytical and experimental results obtained from testing prototype planar motors.

  6. Diagnostic findings in 132 great horned owls

    USGS Publications Warehouse

    Franson, J.C.; Little, S.E.

    1996-01-01

    We reviewed diagnostic findings for 132 great horned owl (Bubo virginianus) carcasses that were submitted to the National Wildlife Health Center from 1975-93. The carcasses were collected in 24 states but most came from Colorado (N = 21), Missouri (N = 12), Oregon (N = 12), Wyoming (N = 11), Illinois (N = 10), and Wisconsin (N = 9). Forty-two birds were emaciated but presumptive causes of emaciation, including old injuries, chronic lesions in various organs, and exposure to dieldrin, were found in only 16. A greater proportion of juveniles (56%) than adults (29%) were emaciated. Twelve owls were shot and 35 died from other traumatic injuries. Poisonings were diagnosed in 11 birds, including five associated with hydrogen sulfide exposure in oil fields and six cases of agricultural pesticide poisonings. Electrocution killed nine birds and infectious diseases were found in six. Miscellaneous conditions, including egg impaction, drowning, and visceral gout were diagnosed in three of the birds and the cause of death was undetermined in 14 owls. While this review identifies major diagnostic findings in great horned owls, sample bias prevents definitive conclusions regarding actual proportional causes of mortality.

  7. Bioprospecting for podophyllotoxin in the Big Horn Mountains, Wyoming

    USDA-ARS?s Scientific Manuscript database

    The objective of this study was to evaluate variations in podophyllotoxin concentrations in Juniperus species found in the Big Horn Mountains in Wyoming. It was found that Juniperus species in the Big Horn Mountains included three species; J. communis L. (common juniper), J. horizontalis Moench. (c...

  8. The corrugated horn as an antenna range standard.

    NASA Technical Reports Server (NTRS)

    Caldecott, R.; Mentzer, C. A.; Peters, L., Jr.

    1973-01-01

    The corrugated horn is discussed as is a valuable tool for use in microwave pattern ranges. It has the properties of concentrated energy in the main beam, low backlobes, high efficiency, almost monotonic amplitude, and phase radiation patterns which make the corrugated horn useful for a source antenna in a pattern range and also as a possible standard antenna for calibration purposes.

  9. Idiopathic prolapse of 1 uterine horn in a yearling filly

    PubMed Central

    2004-01-01

    Abstract A yearling filly was presented for protrusion of a mass at the vulvar margins. A diagnosis of prolapse of the right uterine horn was made after vaginoscopy, transrectal palpation, and ultrasonography. It was confirmed later by biopsy of the tissue. Recovery was uneventful after easy replacement of the uterine horn. PMID:15317392

  10. 30 CFR 56.14132 - Horns and backup alarms.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... Equipment Safety Devices and Maintenance Requirements § 56.14132 Horns and backup alarms. (a) Manually-operated horns or other audible warning devices provided on self-propelled mobile equipment as a safety... the rear, self-propelled mobile equipment shall have— (i) An automatic reverse-activated signal alarm...

  11. 30 CFR 56.14132 - Horns and backup alarms.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... Equipment Safety Devices and Maintenance Requirements § 56.14132 Horns and backup alarms. (a) Manually-operated horns or other audible warning devices provided on self-propelled mobile equipment as a safety... the rear, self-propelled mobile equipment shall have— (i) An automatic reverse-activated signal alarm...

  12. A robust mathematical model for a loophole-free Clauser-Horne experiment

    NASA Astrophysics Data System (ADS)

    Bierhorst, Peter

    2015-05-01

    Recent experiments (Giustina et al 2013 Nature 497 227-30 Christensen et al 2013 Phys. Rev. Lett. 111 130406) have reached detection efficiencies sufficient to close the detection loophole, testing the Clauser-Horne version of Bell's inequality. For a similar future experiment to be completely loophole-free, it will be important to have discrete experimental trials with randomized measurement settings for each trial, and the statistical analysis should not overlook the possibility of a local state varying over time with possible dependence on earlier trials (the ‘memory loophole’). In this paper, a mathematical model for such an experiment is presented, and a method for statistical analysis that is robust to memory effects is introduced. Additionally, a new method for calculating exact p-values for martingale-based statistics is described; previously, only non-sharp upper bounds derived from the Azuma-Hoeffding inequality have been available for such statistics. This improvement decreases the required number of experimental trials to demonstrate non-locality. The statistical techniques are applied to the data of Giustina et al (2013 Nature 497 227-30) and Christensen et al (2013 Phys. Rev. Lett. 111 130406) and found to perform well.

  13. The Limits of Offshore Balancing

    DTIC Science & Technology

    2015-09-01

    UNITED STATES ARMY WAR COLLEGE PRESS Carlisle Barracks, PA ST R ENGTH-’W I SDOM THE LIMITS OF OFFSHORE BALANCING Hal Brands U.S. ARMY WAR...Offshore Balancing 5a. CONTRACT NUMBER 5b. GRANT NUMBER 5c. PROGRAM ELEMENT NUMBER 6. AUTHOR(S) 5d. PROJECT NUMBER 5e. TASK NUMBER 5f. WORK UNIT...Strategic Studies Institute and U.S. Army War College Press THE LIMITS OF OFFSHORE BALANCING Hal Brands September 2015

  14. Insecticide resistance in the horn fly: alternative control strategies.

    PubMed

    Oyarzún, M P; Quiroz, A; Birkett, M A

    2008-09-01

    The horn fly, Haematobia irritans (Linnaeus 1758) (Diptera: Muscidae) is one of the most widespread and economically important pests of cattle. Although insecticides have been used for fly control, success has been limited because of the development of insecticide resistance in all countries where the horn fly is found. This problem, along with public pressure for insecticide-free food and the prohibitive cost of developing new classes of compounds, has driven the investigation of alternative control methods that minimize or avoid the use of insecticides. This review provides details of the economic impact of horn flies, existing insecticides used for horn fly control and resistance mechanisms. Current research on new methods of horn fly control based on resistant cattle selection, semiochemicals, biological control and vaccines is also discussed.

  15. Probe impedance measurements for millimeter-wave integrated horn antennas

    NASA Technical Reports Server (NTRS)

    Guo, Yong; Chiao, Jung-Chih; Potter, Kent A.; Rutledge, David B.

    1993-01-01

    In order to achieve an impedance-matched millimeter-wave integrated horn antenna mixer array, the characteristics of the antenna probes inside the horn must be known. This paper describes impedance measurements for various probes in low-frequency model horns of two different types: (1) a 3 x 3 array made of aluminum by electric discharge machining and (2) a half horn made of copper sheet placed on a big copper-clad circuit board that was used as an image plane. The results of measurements indicate that the presence of the horn increases the effective length of the probe element, in agreement with reports of Guo et al. (1991) and theoretical analysis of Eleftheriades et al. (1991). It was also found that the resonant frequencies can be controlled by changing the length of the probes or by loading the probes.

  16. Reflectance of acoustic horns and solution of the inverse problem

    PubMed Central

    Rasetshwane, Daniel M.; Neely, Stephen T.; Allen, Jont B.; Shera, Christopher A.

    2012-01-01

    A method is described for solving the inverse problem of determining the profile of an acoustic horn when time-domain reflectance (TDR) is known only at the entrance. The method involves recasting Webster’s horn equation in terms of forward and backward propagating wave variables. An essential feature of this method is a requirement that the backward propagating wave be continuous at the wave-front at all locations beyond the entrance. Derivation of the inverse solution raises questions about the meaning of causality in the context of wave propagation in non-uniform tubes. Exact reflectance expressions are presented for infinite exponential, conical and parabolic horns based on exact solutions of the horn equation. Diameter functions obtained with the inverse solution are a good match to all three horn profiles. PMID:22423684

  17. Functional genomics of the horn fly, Haematobia irritans (Linnaeus, 1758)

    PubMed Central

    2011-01-01

    Background The horn fly, Haematobia irritans (Linnaeus, 1758) (Diptera: Muscidae) is one of the most important ectoparasites of pastured cattle. Horn flies infestations reduce cattle weight gain and milk production. Additionally, horn flies are mechanical vectors of different pathogens that cause disease in cattle. The aim of this study was to conduct a functional genomics study in female horn flies using Expressed Sequence Tags (EST) analysis and RNA interference (RNAi). Results A cDNA library was made from whole abdominal tissues collected from partially fed adult female horn flies. High quality horn fly ESTs (2,160) were sequenced and assembled into 992 unigenes (178 contigs and 814 singlets) representing molecular functions such as serine proteases, cell metabolism, mitochondrial function, transcription and translation, transport, chromatin structure, vitellogenesis, cytoskeleton, DNA replication, cell response to stress and infection, cell proliferation and cell-cell interactions, intracellular trafficking and secretion, and development. Functional analyses were conducted using RNAi for the first time in horn flies. Gene knockdown by RNAi resulted in higher horn fly mortality (protease inhibitor functional group), reduced oviposition (vitellogenin, ferritin and vATPase groups) or both (immune response and 5'-NUC groups) when compared to controls. Silencing of ubiquitination ESTs did not affect horn fly mortality and ovisposition while gene knockdown in the ferritin and vATPse functional groups reduced mortality when compared to controls. Conclusions These results advanced the molecular characterization of this important ectoparasite and suggested candidate protective antigens for the development of vaccines for the control of horn fly infestations. PMID:21310032

  18. Descriptions of Actinoplanes ianthinogenes nom. rev. and Actinoplanes octamycinicus corrig. comb. nov., nom. rev.

    PubMed

    Tamura, Tomohiko; Ishida, Yuumi; Suzuki, Ken-ichiro

    2011-12-01

    Phylogenetic analysis of 'Actinoplanes ianthinogenes' Coronelli et al. 1974 and 'Actinoplanes ianthinogenes subsp. octamycini' Gauze et al. 1979 based on 16S rRNA gene sequencing data revealed that these organisms form a clade in the family Micromonosporaceae. Morphological and chemotaxonomic characteristics of strains of these species were consistent with those of members of the genus Actinoplanes. Morphological, DNA-DNA hybridization, physiological, biochemical and chemotaxonomic data showed that 'A. ianthinogenes' and 'A. ianthinogenes subsp. octamycini' can be easily differentiated from each other and that they merit separate species status. On the basis of morphological, physiological, biochemical, chemotaxonomic and DNA-DNA hybridization data, it is concluded that 'A. ianthinogenes' and 'A. ianthinogenes subsp. octamycini' should be assigned the status of two novel species: Actinoplanes ianthinogenes nom. rev. (type strain NBRC 13996(T)=A/1668(T)=ATCC 21884(T)=BCRC 13611(T)=DSM 43864(T)=IMSNU 20032(T)=JCM 3249(T)=KCTC 9347(T)=KCTC 9592(T)=NCIMB 12639(T)=NRRL B-16720(T)) and Actinoplanes octamycinicus corrig. comb. nov., nom. rev. (type strain NBRC 14524(T)=INA 4041(T)=ATCC 43632(T)=JCM 9649(T)=KCTC 9593(T)), respectively.

  19. Marine and offshore corrosion

    SciTech Connect

    Chandler, K.A.

    1985-01-01

    Until recently marine corrosion technology has been preoccupied with the corrosion of ships. Within the last ten years, however, the rapid expansion of oil and gas exploration has changed the course of corrosion research as well as the market for corrosion services and products. So complete has been the change that a new approach, dealing with ships, structures, and plant has been taken in this book. This introduction to the control of corrosion in marine environments will serve as a reference on topics ranging from coating systems to metallurgical considerations in the design of ships, offshore structure, plant and pipelines.

  20. A nonrational B-spline profiled horn with high displacement amplification for ultrasonic welding.

    PubMed

    Nguyen, Huu-Tu; Nguyen, Hai-Dang; Uan, Jun-Yen; Wang, Dung-An

    2014-12-01

    A new horn with high displacement amplification for ultrasonic welding is developed. The profile of the horn is a nonrational B-spline curve with an open uniform knot vector. The ultrasonic actuation of the horn exploits the first longitudinal displacement mode of the horn. The horn is designed by an optimization scheme and finite element analyses. Performances of the proposed horn have been evaluated by experiments. The displacement amplification of the proposed horn is 41.4% and 8.6% higher than that of the traditional catenoidal horn and a Bézier-profile horn, respectively, with the same length and end surface diameters. The developed horn has a lower displacement amplification than the nonuniform rational B-spline profiled horn but a much smoother stress distribution. The developed horn, the catenoidal horn, and the Bézier horn are fabricated and used for ultrasonic welding of lap-shear specimens. The bonding strength of the joints welded by the open uniform nonrational B-spline (OUNBS) horn is the highest among the three horns for the various welding parameters considered. The locations of the failure mode and the distribution of the voids of the specimens are investigated to explain the reason of the high bonding strength achieved by the OUNBS horn.

  1. Biallelic inactivation of REV7 is associated with Fanconi anemia

    PubMed Central

    Masliah-Planchon, Julien; Clairmont, Connor; Rousseau, Alix; Ceccaldi, Raphael; Dubois d’Enghien, Catherine; Bluteau, Olivier; Cuccuini, Wendy; Gachet, Stéphanie; Peffault de Latour, Régis; Leblanc, Thierry; Socié, Gérard; Baruchel, André; Stoppa-Lyonnet, Dominique; D’Andrea, Alan D.

    2016-01-01

    Fanconi anemia (FA) is a recessive genetic disease characterized by congenital abnormalities, chromosome instability, progressive bone marrow failure (BMF), and a strong predisposition to cancer. Twenty FA genes have been identified, and the FANC proteins they encode cooperate in a common pathway that regulates DNA crosslink repair and replication fork stability. We identified a child with severe BMF who harbored biallelic inactivating mutations of the translesion DNA synthesis (TLS) gene REV7 (also known as MAD2L2), which encodes the mutant REV7 protein REV7-V85E. Patient-derived cells demonstrated an extended FA phenotype, which included increased chromosome breaks and G2/M accumulation upon exposure to DNA crosslinking agents, γH2AX and 53BP1 foci accumulation, and enhanced p53/p21 activation relative to cells derived from healthy patients. Expression of WT REV7 restored normal cellular and functional phenotypes in the patient’s cells, and CRISPR/Cas9 inactivation of REV7 in a non-FA human cell line produced an FA phenotype. Finally, silencing Rev7 in primary hematopoietic cells impaired progenitor function, suggesting that the DNA repair defect underlies the development of BMF in FA. Taken together, our genetic and functional analyses identified REV7 as a previously undescribed FA gene, which we term FANCV. PMID:27500492

  2. HIV-1 rev depolymerizes microtubules to form stable bilayered rings.

    PubMed

    Watts, N R; Sackett, D L; Ward, R D; Miller, M W; Wingfield, P T; Stahl, S S; Steven, A C

    2000-07-24

    We describe a novel interaction between HIV-1 Rev and microtubules (MTs) that results in the formation of bilayered rings that are 44-49 nm in external diameter, 3.4-4.2 MD (megadaltons) in mass, and have 28-, 30-, or 32-fold symmetry. Ring formation is not sensitive to taxol, colchicine, or microtubule-associated proteins, but requires Mg(2+) and is inhibited by maytansine. The interaction involves the NH(2)-terminal domain of Rev and the face of tubulin exposed on the exterior of the MTs. The NH(2)-terminal half of Rev has unexpected sequence similarity to the tubulin-binding portion of the catalytic/motor domains of the microtubule-destabilizing Kin I kinesins. We propose a model wherein binding of Rev dimers to MTs at their ends causes segments of two neighboring protofilaments to peel off and close into rings, circumferentially containing 14, 15, or 16 tubulin heterodimers, with Rev bound on the inside. Rev has a strong inhibitory effect on aster formation in Xenopus egg extracts, demonstrating that it can interact with tubulin in the presence of normal levels of cellular constituents. These results suggest that Rev may interact with MTs to induce their destabilization, a proposition consistent with the previously described disruption of MTs after HIV-1 infection.

  3. Multisetting Greenberger-Horne-Zeilinger paradoxes

    NASA Astrophysics Data System (ADS)

    Tang, Weidong; Yu, Sixia; Oh, C. H.

    2017-01-01

    The Greenberger-Horne-Zeilinger (GHZ) paradox provides an all-versus-nothing test for the quantum nonlocality. In most of the GHZ paradoxes known so far each observer is allowed to measure only two alternative observables. Here we present a general construction for GHZ paradoxes in which each observer measures more than two observables given that the system is prepared in the n -qudit GHZ state. By doing so we are able to construct a multisetting GHZ paradox for the n -qubit GHZ state, with n being arbitrary, which is genuine n -partite; i.e., no GHZ paradox exists when restricted to a subset of a number of observers for a given set of Mermin observables. Our result fills up the gap of the absence of a genuine GHZ paradox for the GHZ state of an even number of qubits, especially the four-qubit GHZ state as used in GHZ's original proposal.

  4. On the origin of Ammon's horn.

    PubMed

    Iniesta, I

    2014-10-01

    Greek and Roman worship of their gods and myths go back to Ancient Egyptian times. Images engraved in Greco-Roman coinage range from references to the assassination of Caesar and legendary stories like the arrival of a snake shaped demi-god Aesculapius to save the Romans from the plague, to invocations of major deities including Apollo the physician or Ammon the protector. Depicted with the horns of a ram, Ammon was adopted by the Greeks as an epithet of Zeus and later incorporated by the Romans as Jupiter. References to the cult of Ammon appear on tetradrachms minted for Alexander The Great and on provincial Roman coins struck under Claudius. It is thrilling to hold a coin depicting Marcus Aurelius with Salus on the reverse and think that it could have been handed to Galen in payment for his services. However, it is rare to find figures other than rulers on coins and the physician of Pergamum is no exception. Inspired by the Renaissance school of Padua, French anatomists in the Enlightenment (Garengeot in 1742 and Flurant in 1752) continued reviving ancient myths and named the curve-shaped-inner portion of the temporal lobe Ammon's horn. Outstanding scholars who studied this primitive structure of the brain included Lorente de Nó and his mentor Cajal, whose portrait appeared on fifty-pesetas notes issued in 1935. As primary sources of great archaeological and artistic value, Greco-Roman coins provide information about the origins of the myths and gods of classical antiquity and continue to inspire the arts and sciences to this day. Copyright © 2011 Sociedad Española de Neurología. Published by Elsevier Espana. All rights reserved.

  5. Offshore outlook: the American Arctic

    SciTech Connect

    Jahns, M.O.

    1985-05-01

    Offshore areas in the American Arctic are highlighted and the development of the area is compared with other offshore areas where the required technology is more readily available. Principal areas are shown in which new concepts are being put to practice. Canada's east coast is examined. Several technological trends are reviewed to help operators accelerate the discovery and development of arctic petroleum reserves.

  6. Offshore Fish Community: Ecological Interactions

    EPA Science Inventory

    The offshore (>80 m) fish community of Lake Superior is made up of predominately native species. The most prominent species are deepwater sculpin, kiyi, cisco, siscowet lake trout, burbot, and the exotic sea lamprey. Bloater and shortjaw cisco are also found in the offshore zone...

  7. Offshore Fish Community: Ecological Interactions

    EPA Science Inventory

    The offshore (>80 m) fish community of Lake Superior is made up of predominately native species. The most prominent species are deepwater sculpin, kiyi, cisco, siscowet lake trout, burbot, and the exotic sea lamprey. Bloater and shortjaw cisco are also found in the offshore zone...

  8. Object-horning in goitered gazelle: agonistic or marking behaviour?

    PubMed

    Blank, David; Yang, Weikang

    2014-03-01

    We studied object-horning behaviour in goitered gazelles in the natural, arid environment of Kazakhstan over a 6-year period. We found that object-horning was used by adult males mostly as a threat display during territorial conflicts. Therefore object-horning was observed most frequently in territorial single males during the rut in November-December. Object-horning, though, also had a marking effect, with the males' use of this behaviour leaving visible traces that advertized the location of preorbital and urination-defecation scent marks. Therefore, this pattern also was observed linked with preorbital marking and urination-defecation marking behaviours, especially during the rut. Goitered gazelle males chose the most abundant and eatable shrubs for object horning. In contrast to other gazelle species, object-horning in goitered gazelle was observed much more frequently and at the same rate as preorbital and urination-defecation scent markings. This, then, proved a more vigorous and aggressive level of rutting behaviour of the goitered gazelle compared to tropical gazelles, and most likely connected to the short rutting period in the studied species. We concluded, therefore, that object-horning was a manifold phenomenon that played a very important role in goitered gazelle agonistic displays, but without loosing the marking intention of this behaviour.

  9. DNA nuclease activity of Rev-coupled transition metal chelates.

    PubMed

    Joyner, Jeff C; Keuper, Kevin D; Cowan, J A

    2012-06-07

    Artificial nucleases containing Rev-coupled metal chelates based on combinations of the transition metals Fe(2+), Co(2+), Ni(2+), and Cu(2+) and the chelators DOTA, DTPA, EDTA, NTA, tripeptide GGH, and tetrapeptide KGHK have been tested for DNA nuclease activity. Originally designed to target reactive transition metal chelates (M-chelates) to the HIV-1 Rev response element mRNA, attachment to the arginine-rich Rev peptide also increases DNA-binding affinity for the attached M-chelates. Apparent K(D) values ranging from 1.7 to 3.6 µM base pairs for binding of supercoiled pUC19 plasmid DNA by Ni-chelate-Rev complexes were observed, as a result of electrostatic attraction between the positively-charged Rev peptide and negatively-charged DNA. Attachment of M-chelates to the Rev peptide resulted in enhancements of DNA nuclease activity ranging from 1-fold (no enhancement) to at least 13-fold (for Cu-DTPA-Rev), for the rate of DNA nicking, with second order rate constants for conversion of DNA(supercoiled) to DNA(nicked) up to 6 × 10(6) M(-1) min(-1), and for conversion of DNA(nicked) to DNA(linear) up to 1 × 10(5) M(-1) min(-1). Freifelder-Trumbo analysis and the ratios of linearization and nicking rate constants (k(lin)/k(nick)) revealed concerted mechanisms for nicking and subsequent linearization of plasmid DNA for all of the Rev-coupled M-chelates, consistent with higher DNA residency times for the Rev-coupled M-chelates. Observed rates for Rev-coupled M-chelates were less skewed by differing DNA-binding affinities than for M-chelates lacking Rev, as a result of the narrow range of DNA-binding affinities observed, and therefore relationships between DNA nuclease activity and other catalyst properties, such as coordination unsaturation, the ability to consume ascorbic acid and generate diffusible radicals, and the identity of the metal center, are now clearly illustrated in light of the similar DNA-binding affinities of all M-chelate-Rev complexes. This work

  10. Pioneering offshore excellence

    SciTech Connect

    Kent, R.P.; Grattan, L.

    1996-11-01

    Hibernia Management and Development Company Ltd. (HMDC) was formed in 1990 by a consortium of oil companies to develop their interests in the Hibernia and Avalon reservoirs offshore Newfoundland in a safe and environmentally responsible manner. The reservoirs are located 315km ESE of St. John`s in the North Atlantic. The water depth is about 80m. The entire Hibernia field is estimated to contain more than three billion barrels of oil in place and the owners development plan area is estimated to contain two billion barrels. Recoverable reserves are estimated to be approximately 615 million barrels. The Hibernia reservoir, the principle reservoir, is located at an average depth of 3,700m. HMDC is building a large concrete gravity based structure (GBS) that which will support the platform drilling and processing facilities and living quarters for 280 personnel. In 1997 the platform will be towed to the production site and production will commence late 1997. Oil will be exported by a 2 km long pipeline to an offshore loading system. Dynamically positioned tankers will then take the oil to market. Average daily production is expected to plateau between 125,000 and 135,000 BOPD. It will be the first major development on the east coast of Canada and is located in an area that is prone to pack ice and icebergs.

  11. A 20-dB quasi-integrated horn antenna

    NASA Technical Reports Server (NTRS)

    Eleftheriades, George V.; Ali-Ahmad, Walid Y.; Rebeiz, Gabriel M.

    1992-01-01

    A multimode quasi-integrated dipole-fed horn antenna is presented with a performance comparable to that of waveguide-fed corrugated horn antennas. The antenna has been designed using fullwave analysis and has been fabricated and tested at 91 GHz. The horn has a gain of 20 dB with very symmetric patterns, a Gaussian coupling efficiency of 97 percent, and a cross-polarization level of -22.7 dB. The antenna provides a significant improvement in integrated antenna designs and is suitable for millimeter-wave communication and radar systems and as a Gaussian-beam launcher in quasi-optical receiver systems.

  12. Understanding recent eastern Horn of Africa rainfall variability and change

    USGS Publications Warehouse

    Liebmann, Brant; Hoerling, Martin P.; Funk, Christopher C.; Blade, Ileana; Dole, Randall M.; Allured, Dave; Quan, Xiaowei; Eischeid, Jon K.

    2014-01-01

    The recent upward trend in the October–December wet season is rather weak, however, and its statistical significance is compromised by strong year-to-year fluctuations. October–December eastern Horn rain variability is strongly associated with El Niño–Southern Oscillation and Indian Ocean dipole phenomena on interannual scales, in both model and observations. The interannual October–December correlation between the ensemble-average and observed Horn rainfall 0.87. By comparison, interannual March–May Horn precipitation is only weakly constrained by SST anomalies.

  13. Formal verification of communication protocols using quantized Horn clauses

    NASA Astrophysics Data System (ADS)

    Balu, Radhakrishnan

    2016-05-01

    The stochastic nature of quantum communication protocols naturally lends itself for expression via probabilistic logic languages. In this work we describe quantized computation using Horn clauses and base the semantics on quantum probability. Turing computable Horn clauses are very convenient to work with and the formalism can be extended to general form of first order languages. Towards this end we build a Hilbert space of H-interpretations and a corresponding non commutative von Neumann algebra of bounded linear operators. We demonstrate the expressive power of the language by casting quantum communication protocols as Horn clauses.

  14. Analysis of the influence of subcellular localization of the HIV Rev protein on Rev-dependent gene expression by multi-fluorescence live-cell imaging

    SciTech Connect

    Wolff, Horst; Hadian, Kamyar; Ziegler, Manja; Weierich, Claudia; Kramer-Hammerle, Susanne; Kleinschmidt, Andrea; Erfle, Volker; Brack-Werner, Ruth . E-mail: brack@gsf.de

    2006-02-15

    The human immunodeficiency virus Rev protein is a post-transcriptional activator of HIV gene expression. Rev is a nucleocytoplasmic shuttle protein that displays characteristic nuclear/nucleolar subcellular localization in various cell lines. Cytoplasmic localization of Rev occurs under various conditions disrupting Rev function. The goal of this study was to investigate the relationship between localization of Rev and its functional activity in living cells. A triple-fluorescent imaging assay, called AQ-FIND, was established for automatic quantitative evaluation of nucleocytoplasmic distribution of fluorescently tagged proteins. This assay was used to screen 500 rev genes generated by error-prone PCR for Rev mutants with different localization phenotypes. Activities of the Rev mutants were determined with a second quantitative, dual-fluorescent reporter assay. In HeLa cells, the majority of nuclear Rev mutants had activities similar to wild-type Rev. The activities of Rev mutants with abnormal cytoplasmic localization ranged from moderately impaired to nonfunctional. There was no linear correlation between subcellular distribution and levels of Rev activity. In astrocytes, nuclear Rev mutants showed similar impaired activities as the cytoplasmic wild-type Rev. Our data suggest that steady-state subcellular localization is not a primary regulator of Rev activity but may change as a secondary consequence of altered Rev function. The methodologies described here have potential for studying the significance of subcellular localization for functions of other regulatory factors.

  15. Regulation of Rev1 by the Fanconi Anemia Core Complex

    PubMed Central

    Kim, Hyungjin; Yang, Kailin; Dejsuphong, Donniphat; D’Andrea, Alan D.

    2011-01-01

    The fifteen known Fanconi Anemia (FA) proteins cooperate in a pathway which regulates DNA interstrand crosslink repair. Recent studies indicate that the FA pathway also controls Rev1-mediated translesion DNA synthesis (TLS). Here we identify a novel protein FAAP20, which is an integral subunit of the multisubunit FA core complex. FAAP20 binds to FANCA subunit and is required for complex stability and monoubiquitination of FANCD2. FAAP20 contains a UBZ4 (Ubiquitin Binding Zinc finger 4) domain and binds to the monoubiquitinated form of Rev1. FAAP20 binding stabilizes Rev1 nuclear foci and promotes the interaction of the FA core with PCNA/Rev1 DNA damage bypass complexes. FAAP20 therefore provides a critical link between the FA pathway and TLS polymerase activity. We propose that the FA core complex regulates crosslink repair, by channeling lesions to damage bypass pathways and preventing large DNA insertions and deletions. PMID:22266823

  16. Altered Sleep Homeostasis in Rev-erbα Knockout Mice.

    PubMed

    Mang, Géraldine M; La Spada, Francesco; Emmenegger, Yann; Chappuis, Sylvie; Ripperger, Jürgen A; Albrecht, Urs; Franken, Paul

    2016-03-01

    The nuclear receptor REV-ERBα is a potent, constitutive transcriptional repressor critical for the regulation of key circadian and metabolic genes. Recently, REV-ERBα's involvement in learning, neurogenesis, mood, and dopamine turnover was demonstrated suggesting a specific role in central nervous system functioning. We have previously shown that the brain expression of several core clock genes, including Rev-erbα, is modulated by sleep loss. We here test the consequences of a loss of REV-ERBα on the homeostatic regulation of sleep. EEG/EMG signals were recorded in Rev-erbα knockout (KO) mice and their wild type (WT) littermates during baseline, sleep deprivation, and recovery. Cortical gene expression measurements after sleep deprivation were contrasted to baseline. Although baseline sleep/wake duration was remarkably similar, KO mice showed an advance of the sleep/wake distribution relative to the light-dark cycle. After sleep onset in baseline and after sleep deprivation, both EEG delta power (1-4 Hz) and sleep consolidation were reduced in KO mice indicating a slower increase of homeostatic sleep need during wakefulness. This slower increase might relate to the smaller increase in theta and gamma power observed in the waking EEG prior to sleep onset under both conditions. Indeed, the increased theta activity during wakefulness predicted delta power in subsequent NREM sleep. Lack of Rev-erbα increased Bmal1, Npas2, Clock, and Fabp7 expression, confirming the direct regulation of these genes by REV-ERBα also in the brain. Our results add further proof to the notion that clock genes are involved in sleep homeostasis. Because accumulating evidence directly links REV-ERBα to dopamine signaling the altered homeostatic regulation of sleep reported here are discussed in that context. © 2016 Associated Professional Sleep Societies, LLC.

  17. Specific binding of a basic peptide from HIV-1 Rev.

    PubMed Central

    Kjems, J; Calnan, B J; Frankel, A D; Sharp, P A

    1992-01-01

    Human immunodeficiency virus type I (HIV-1) encodes a regulatory protein, Rev, which is required for cytoplasmic expression of incompletely spliced viral mRNA. Rev activity is mediated through specific binding to a cis-acting Rev responsive element (RRE) located within the env region of HIV-1. A monomer Rev binding site corresponding to 37 nucleotides of the RRE (IIB RNA) was studied by RNA footprinting, modification interference experiments and mutational analysis. Surprisingly, a 17 amino acid peptide, corresponding to the basic domain of Rev, binds specifically to this site at essentially identical nucleotides and probably induces additional base pairing. The Rev protein and related peptide interact primarily with two sets of nucleotides located at the junction of single and double stranded regions, and at an additional site located within a helix. This suggests that the domains of proteins responsible for specific RNA binding can be remarkably small and that the interaction between RNA and protein can probably induce structure in both constituents. Images PMID:1547776

  18. Bilateral segmental aplasia with unilateral uterine horn torsion in a Pomeranian bitch.

    PubMed

    Nakamura, Kensuke; Yamasaki, Masahiro; Osaki, Tomohiro; Ohta, Hiroshi; Sasaki, Noboru; Aoshima, Keisuke; Kimura, Takashi; Takiguchi, Mitsuyoshi

    2012-01-01

    Bilateral segmental aplasia of the uterine horns with unilateral pyometra and uterine horn torsion were diagnosed in a Pomeranian bitch that presented with chronic abdominal distension and an acute onset of anorexia and lethargy. Because radiographic and ultrasonographic findings revealed the presence of markedly enlarged bilateral uterine horns filled with fluid in the caudal abdomen, a tentative diagnosis of either pyometra or hydrometra with uterine horn torsion was made. Exploratory laparotomy showed bilateral, segmentally distended uterine horns with unilateral uterine horn torsion. Ovariohysterectomy was performed, and bilateral segmental aplasia of the uterine horns with the development of unilateral uterine horn torsion was diagnosed histopathologically. To the authors' knowledge, this is the first report of uterine horn torsion in conjunction with segmental aplasia of the uterine horn in a bitch.

  19. Bahrain's offshore banking center

    SciTech Connect

    Gerakis, A.S.; Roncesvalles, O.

    1983-01-01

    The economic effects of Bahrain's schemes for licensing offshore banking units (OBUs) were the immediate response of major international banks and the financial services the banking center has rendered by improving regional money and exchange markets at a time when a Middle East link was needed to service the increasing demand for oil-wealth banking services. Bahrain's leadership also created a favorable climate. Aggressive competition from banks in Kuwait and Saudi Arabia have caused some friction, but informal supervision by the Bahrain Monetary Agency (BMA) should be able to avoid serious difficulty. Bahrain's success required a banking infrastructure, a free-enterprise system, a willingness to maintain banking standards, a country small enough to benefit directly from OBU income, and a gap in nearby competing centers. 39 references, 1 figure, 5 tables. (DCK)

  20. The limits on combining recursive horn rules with description logics

    SciTech Connect

    Levy, A.Y.; Rousset, M.C.

    1996-12-31

    Horn rule languages have formed the basis for many Artificial Intelligence application languages, but are not expressive enough to model domains with a rich hierarchical structure. Description logics have been designed especially to model rich hierarchies. Several applications would significantly benefit from combining the expressive power of both formalisms. This paper focuses on combining recursive function-free Horn rules with the expressive description logic ALCNR, and shows exactly when a hybrid language with decidable inference can be obtained. First, we show that several of the core constructors of description logics lead by themselves to undecidability of inference when combined with recursive function-free Horn rules. We then show that without these constructors we obtain a maximal subset of ALCNRR that yields a decidable hybrid language. Finally, we describe a restriction on the Horn rules that guarantees decidable inference when combined with all of ALCNR, and covers many of the common usages of recursive rules.

  1. Integrated horn antennas for millimeter-wave applications

    NASA Astrophysics Data System (ADS)

    Rebeiz, Gabriel M.; Katehi, Linda P. B.; Ali-Ahmad, Walid Y.; Eleftheriades, George V.; Ling, Curtis C.

    1992-02-01

    The development of integrated horn antennas since their introduction in 1987 is reviewed. The integrated horn is fabricated by suspending a dipole antenna, on a thin dielectric membrane, in a pyramidal cavity etched in silicon. Recent progress has resulted in optimized low- and high-gain designs, with single and double polarization for remote-sensing and communication applications. A full-wave analysis technique has resulted in an integrated antenna with performance comparable to that of waveguide-fed corrugated-horn antennas. The integrated horn design can be extended to large arrays, for imaging and phased-array applications, while leaving plenty of room for the RF and IF processing circuitry. Theoretical and experimental results at microwave frequencies and at 90 GHz, 240 GHz, and 802 GHz are presented.

  2. Ultra-wideband horn antenna with abrupt radiator

    SciTech Connect

    McEwan, Thomas E.

    1998-01-01

    An ultra-wideband horn antenna transmits and receives impulse waveforms for short-range radars and impulse time-of flight systems. The antenna reduces or eliminates various sources of close-in radar clutter, including pulse dispersion and ringing, sidelobe clutter, and feedline coupling into the antenna. Dispersion is minimized with an abrupt launch point radiator element; sidelobe and feedline coupling are minimized by recessing the radiator into a metallic horn. Low frequency cut-off associated with a horn is extended by configuring the radiator drive impedance to approach a short circuit at low frequencies. A tapered feed plate connects at one end to a feedline, and at the other end to a launcher plate which is mounted to an inside wall of the horn. The launcher plate and feed plate join at an abrupt edge which forms the single launch point of the antenna.

  3. 28. Photograph of interior view of fog horn signal building ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    28. Photograph of interior view of fog horn signal building with two lighthouse keepers by steam engine wheel, ca. 1920. - Block Island Southeast Light, Spring Street & Mohegan Trail at Mohegan Bluffs, New Shoreham, Washington County, RI

  4. Large loudspeaker horns and crated Edison radios from 1929 are ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    Large loudspeaker horns and crated Edison radios from 1929 are stored in a side room on the third floor. - Thomas A. Edison Laboratories, Building No. 5, Main Street & Lakeside Avenue, West Orange, Essex County, NJ

  5. Image measurement technique on vibration amplitude of ultrasonic horn

    NASA Astrophysics Data System (ADS)

    Zhang, Yong-bin; Wu, Zhi-qun; Zhu, Jian-ping; He, Jian-guo; Liu, Guang-min

    2013-10-01

    The paper proposes a method to measure vibration amplitude of ultrasonic horn which is a very important component in the spindle for micro-electrical-chemical discharging machining. The method of image measuring amplitude on high frequency vibration is introduced. Non-contact measurement system based on vision technology is constructed. High precision location algorithm on image centroid, quadratic location algorithm, is presented to find the center of little light spot. Measurement experiments have been done to show the effect of image measurement technique on vibration amplitude of ultrasonic horn. In the experiments, precise calibration of the vision system is implemented using a normal graticule to obtain the scale factor between image pixel and real distance. The vibration amplitude of ultrasonic horn is changed by modifying the voltage amplitude of pulse power supply. The image of feature on ultrasonic horn is captured and image processing is carried out. The vibration amplitudes are got at different voltages.

  6. Ultra-wideband horn antenna with abrupt radiator

    DOEpatents

    McEwan, T.E.

    1998-05-19

    An ultra-wideband horn antenna transmits and receives impulse waveforms for short-range radars and impulse time-of flight systems. The antenna reduces or eliminates various sources of close-in radar clutter, including pulse dispersion and ringing, sidelobe clutter, and feedline coupling into the antenna. Dispersion is minimized with an abrupt launch point radiator element; sidelobe and feedline coupling are minimized by recessing the radiator into a metallic horn. Low frequency cut-off associated with a horn is extended by configuring the radiator drive impedance to approach a short circuit at low frequencies. A tapered feed plate connects at one end to a feedline, and at the other end to a launcher plate which is mounted to an inside wall of the horn. The launcher plate and feed plate join at an abrupt edge which forms the single launch point of the antenna. 8 figs.

  7. A comparative study of corrugated horn design by evolutionary techniques

    NASA Technical Reports Server (NTRS)

    Hoorfar, A.

    2003-01-01

    Here an evolutionary programming algorithm is used to optimize the pattern of a corrugated circular horn subject to various constraints on return loss, antenna beamwidth, pattern circularity, and low cross polarization.

  8. Water resources of Big Horn County, Wyoming

    USGS Publications Warehouse

    Plafcan, Maria; Cassidy, E.W.; Smalley, M.L.

    1993-01-01

    Groundwater in unconsolidated aquifers is the most reliable and accessible source of potable water in Big Horn County, Wyoming. Well yields generally ranged from 25 to 200 gal/min; however, yields of 1600 gal/min are reported from wells in the gravel, pediment, and fan deposits. Bedrock aquifers that yield the most abundant water supplies are the Tensleep Sandstone, Madison Limestone, Bighorn Dolomite, and Flathead Sandstone. The Madison Limestone, the Darby Formation, and the Bighorn Dolomite form the Madison/Bighorn aquifer. Shut-in pressure from flowing wells in bedrock indicate declines, from the time the wells were completed to 1988, by as much as 390 ft. Water samples from wells completed,in unconsolidated aquifers had concentration of dissolved solids less than 2000 mg/L. Water samples from wells in aquifers in Paleozoic and Precambrian rocks had median concentrations of dissolved solids ranging from 111 to 275 mg/L. Perennial streams originate in the mountains and ephemeral streams originate in the Bighorn Basin. The predominant dissolved constituents are calcium or sodium and bicarbonate or sulfate. Concentrations of pesticides detected in surface-water samples were less than the U.S. Environmental Protection Agency (USEPA) maximum contaminant levels. Pesticides detected in groundwater samples included dicamba and picloram at a concentration of 0.40 microg/L, atrazines (0.40 microg/L), aldicarb sulfoxide (1.44 microg/L), aldicarb sulfoxide (0.52 microg/L), and malathion (0.02 microg/L). Analyses of groundwater samples for radionuclides indicated that concentrations from four municipal wells exceeded the maximum contaminant levels established by the USEPA. Surface water accounts for 96 percent and groundwater accounts for 4 percent of total off-stream water use in Big Horn County. Irrigation is the largest off-stream use of both surface and groundwater. Groundwater supplies 89 percent of water used for domestic purposes and about 16 percent of water used

  9. Design and layout of rectangular waveguide horns antennas

    NASA Astrophysics Data System (ADS)

    Fasold, D.; Pecher, H.

    Theoretical basis of waveguide horn antennas (WHA) is outlined. Applicable design curves, such as nomograms for determining gain and a diagram for laying out necessary waveguide dimensions, are given. Use of WHA designs in satellite radio and television systems is discussed. Antenna radiation pattern calculations are treated and optimization problems are dealt with. Based on design feasibility results, a laboratory model of a transmitter antenna for TV-SAT was built. The equipment, an elliptical waveguide horn design, demonstrates satisfactory performance.

  10. Pyogenic granuloma underlying cutaneous horn in a young boy

    PubMed Central

    Nair, Pragya A.; Kota, Rahul Krishna S.; Pilani, Abhisheik P.

    2016-01-01

    Cutaneous horn is an elongated, keratinous projection that usually occurs over the sun-exposed areas. It is a clinical diagnosis and may overlie any benign, premalignant, or malignant conditions. Treatment includes wide surgical excision with careful histological examination to exclude a focus of malignancy. An unusual case of a pyogenic granuloma presenting as cutaneous horn on the lower lip in an 11-year-old boy is presented here. PMID:27057494

  11. Ice interaction with offshore structures

    SciTech Connect

    Cammaert, A.B.; Muggeridge, D.B.

    1988-01-01

    Oil platforms and other offshore structures being built in the arctic regions must be able to withstand icebergs, ice islands, and pack ice. This reference explain the effect ice has on offshore structures and demonstrates design and construction methods that allow such structures to survive in harsh, ice-ridden environments. It analyzes the characteristics of sea ice as well as dynamic ice forces on structures. Techniques for ice modeling and field testing facilitate the design and construction of sturdy, offshore constructions. Computer programs included.

  12. WIND Toolkit Offshore Summary Dataset

    DOE Data Explorer

    Draxl, Caroline; Musial, Walt; Scott, George; Phillips, Caleb

    2017-08-18

    This dataset contains summary statistics for offshore wind resources for the continental United States derived from the Wind Integration National Datatset (WIND) Toolkit. These data are available in two formats: GDB - Compressed geodatabases containing statistical summaries aligned with lease blocks (aliquots) stored in a GIS format. These data are partitioned into Pacific, Atlantic, and Gulf resource regions. HDF5 - Statistical summaries of all points in the offshore Pacific, Atlantic, and Gulf offshore regions. These data are located on the original WIND Toolkit grid and have not been reassigned or downsampled to lease blocks. These data were developed under contract by NREL for the Bureau of Oceanic Energy Management (BOEM).

  13. Proflavine acts as a Rev inhibitor by targeting the high-affinity Rev binding site of the Rev responsive element of HIV-1.

    PubMed

    DeJong, Eric S; Chang, Chia-en; Gilson, Michael K; Marino, John P

    2003-07-08

    Rev is an essential regulatory HIV-1 protein that binds the Rev responsive element (RRE) within the env gene of the HIV-1 RNA genome, activating the switch between viral latency and active viral replication. Previously, we have shown that selective incorporation of the fluorescent probe 2-aminopurine (2-AP) into a truncated form of the RRE sequence (RRE-IIB) allowed the binding of an arginine-rich peptide derived from Rev and aminoglycosides to be characterized directly by fluorescence methods. Using these fluorescence and nuclear magnetic resonance (NMR) methods, proflavine has been identified, through a limited screen of selected small heterocyclic compounds, as a specific and high-affinity RRE-IIB binder which inhibits the interaction of the Rev peptide with RRE-IIB. Direct and competitive 2-AP fluorescence binding assays reveal that there are at least two classes of proflavine binding sites on RRE-IIB: a high-affinity site that competes with the Rev peptide for binding to RRE-IIB (K(D) approximately 0.1 +/- 0.05 microM) and a weaker binding site(s) (K(D) approximately 1.1 +/- 0.05 microM). Titrations of RRE-IIB with proflavine, monitored using (1)H NMR, demonstrate that the high-affinity proflavine binding interaction occurs with a 2:1 (proflavine:RRE-IIB) stoichiometry, and NOEs observed in the NOESY spectrum of the 2:1 proflavine.RRE-IIB complex indicate that the two proflavine molecules bind specifically and close to each other within a single binding site. NOESY data further indicate that formation of the 2:1 proflavine.RRE-IIB complex stabilizes base pairing and stacking within the internal purine-rich bulge of RRE-IIB in a manner analogous to what has been observed in the Rev peptide.RRE-IIB complex. The observation that proflavine competes with Rev for binding to RRE-IIB by binding as a dimer to a single high-affinity site opens the possibility for rational drug design based on linking and modifying it and related compounds.

  14. Combating Rhino Horn Trafficking: The Need to Disrupt Criminal Networks

    PubMed Central

    Haas, Timothy C.; Ferreira, Sam M.

    2016-01-01

    The onslaught on the World’s wildlife continues despite numerous initiatives aimed at curbing it. We build a model that integrates rhino horn trade with rhino population dynamics in order to evaluate the impact of various management policies on rhino sustainability. In our model, an agent-based sub-model of horn trade from the poaching event up through a purchase of rhino horn in Asia impacts rhino abundance. A data-validated, individual-based sub-model of the rhino population of South Africa provides these abundance values. We evaluate policies that consist of different combinations of legal trade initiatives, demand reduction marketing campaigns, increased anti-poaching measures within protected areas, and transnational policing initiatives aimed at disrupting those criminal syndicates engaged in horn trafficking. Simulation runs of our model over the next 35 years produces a sustainable rhino population under only one management policy. This policy includes both a transnational policing effort aimed at dismantling those criminal networks engaged in rhino horn trafficking—coupled with increases in legal economic opportunities for people living next to protected areas where rhinos live. This multi-faceted approach should be the focus of the international debate on strategies to combat the current slaughter of rhino rather than the binary debate about whether rhino horn trade should be legalized. This approach to the evaluation of wildlife management policies may be useful to apply to other species threatened by wildlife trafficking. PMID:27870917

  15. Combating Rhino Horn Trafficking: The Need to Disrupt Criminal Networks.

    PubMed

    Haas, Timothy C; Ferreira, Sam M

    2016-01-01

    The onslaught on the World's wildlife continues despite numerous initiatives aimed at curbing it. We build a model that integrates rhino horn trade with rhino population dynamics in order to evaluate the impact of various management policies on rhino sustainability. In our model, an agent-based sub-model of horn trade from the poaching event up through a purchase of rhino horn in Asia impacts rhino abundance. A data-validated, individual-based sub-model of the rhino population of South Africa provides these abundance values. We evaluate policies that consist of different combinations of legal trade initiatives, demand reduction marketing campaigns, increased anti-poaching measures within protected areas, and transnational policing initiatives aimed at disrupting those criminal syndicates engaged in horn trafficking. Simulation runs of our model over the next 35 years produces a sustainable rhino population under only one management policy. This policy includes both a transnational policing effort aimed at dismantling those criminal networks engaged in rhino horn trafficking-coupled with increases in legal economic opportunities for people living next to protected areas where rhinos live. This multi-faceted approach should be the focus of the international debate on strategies to combat the current slaughter of rhino rather than the binary debate about whether rhino horn trade should be legalized. This approach to the evaluation of wildlife management policies may be useful to apply to other species threatened by wildlife trafficking.

  16. Thirty years of collaboration with Gabriel Horn.

    PubMed

    Bateson, Patrick

    2015-03-01

    All the collaborative work described in this review was on the process of behavioural imprinting occurring early in the life of domestic chicks. Finding a link between learning and a change in the brain was only a first step in establishing a representation of the imprinting object. A series of overlapping experiments were necessary to eliminate alternative explanations. Once completed, a structure, the intermediate and medial mesopallium (IMM), was found to be strongly linked to the formation of a neural representation of the object used for imprinting the birds. With the site identified, lesion experiments showed that it was necessary for imprinting but not associative learning. Also the two sides of the brain responded differently with the left IMM acting as a permanent store and the right side acting as a way station to other parts of the brain. The collaborative work led to many studies by Gabriel Horn with others on the molecular and cellular bases of imprinting, and also to neural net modelling and behavioural studies with me on the nature of category formation in intact animals.

  17. Restoration of the Golden Horn Estuary (Halic).

    PubMed

    Coleman, Heather M; Kanat, Gurdal; Aydinol Turkdogan, F Ilter

    2009-12-01

    Restoration of the iconic Golden Horn Estuary in Istanbul, Turkey was a substantial political, logistical, ecological, and social challenge. Forty years of uncontrolled industrial and urban growth resulted in thick layers of anoxic sediment, toxic bacteria, strong hydrogen sulfide odor, and ecologically unlivable conditions. The major components of restoration, spanning two decades, have included (1) demolition and relocation of industries and homes along the shore, (2) creation of wastewater infrastructure, (3) removal of anoxic sludge from the estuary, (4) removal of a floating bridge that impeded circulation, and (5) creation of cultural and social facilities. Although Turkey is not known as an environmental leader in pollution control, the sum of these efforts was largely successful in revitalizing the area through dramatic water quality improvement. Consequently, the estuary is once again inhabitable for aquatic life as well as amenable to local resource users and foreign visitors, and Istanbul has regained a lost sense of cultural identity. This paper focuses on literature review and personal interviews to discuss the causes of degradation, solutions employed to rehabilitate the estuary, and subsequent physicochemical, ecological, and social changes.

  18. Rethinking the Meaning of "REV" in Modeling Fractured Rock Aquifers

    NASA Astrophysics Data System (ADS)

    Wellman, T. P.; Poeter, E. P.

    2006-12-01

    Discrete fracture network (DFN) models are often useful in characterizing fracture controlled flow and transport, as evidenced by their application in numerous studies over the last 20 years. Collectively, numerical models of fractured aquifers may be coarsely categorized as DFN, continuum, or mixed approach, the latter method representing major features explicitly with the remainder as volume-averaged continuum. A mixed model is often ideal for large-scale fractured aquifers where large features are known deterministically and structural simplification for remaining fractures is helpful to render models computational feasible. The term "REV" (Representative Elementary Volume) is often associated with this simplification. Generally, the classical "REV" approach identifies a single scale at which regions may be represented as continuum, and where parameter values are considered deterministic and scalar. As an alternative, stochastic continuum methodologies allow for distributions of parameter values in a probabilistic framework, and while the scale of continuum is considered important, the classical "REV" definition is not considered entirely relevant. Also, there may be additional influences on "REV" imposed by a model's governing equations, the model parameter(s) of primary interest, and the scale of analysis. Thus, appropriate "REVs" differ depending on the scale at which model predictions are needed to facilitate decision making. For the purpose of evaluating water resources, we provide estimates of basin-scale continuum suitable for managing regional water resources in Turkey Creek Basin near Denver, Colorado using spatial averaging of hydraulic head observations. We also evaluate the commonly discussed requirements of "REV", such as the need for fracture networks to be dense and well connected, and for features to be much smaller than the scale of interest. While these conditions often meet classical "REV" requirements they are neither universally acceptable

  19. Offshore search continues despite disappointments

    SciTech Connect

    Cornitius, T.

    1985-05-01

    Exploration drilling activity in Australia broke records onshore in 1984, but offshore it was a different story. A total of 373 wells were drilled, onshore and offshore, with 266 labeled as wildcats and appraisals. Out of 80 wells drilled offshore last year, 43 were exploratory compared with 49 in 1983; 48 were oil wells, seven were gas, and 25 were dusters. Offshore discoveries included the Talisman 1 off the coast of Western Australia, which tested around 6000 b/d, and Challis 1 in the Timor Sea, which flowed at 5000 b/d. The failure to establish Jabiru in the Timor Sea as a major oil province like Bass Strait was a major disappointment. However, the Challis 1 was a relief since it indicated the presence of a commercial field adjacent to Jabiru.

  20. Regulation of Rev expression by the equine infectious anaemia virus tat-rev mRNA Kozak sequence and its potential influence on viral replication.

    PubMed

    Ma, Jian; Zhang, Zeli; Yao, Qiucheng; Su, Chao; Yin, Xin; Wang, Xiaojun

    2016-09-01

    Rev, an important accessory protein of equine infectious anaemia virus (EIAV), induces the nuclear export of incompletely spliced viral mRNAs. Rev is translated from the tat-rev mRNA through leaky scanning of the tat CUG. In this study, the function of the Kozak sequence at the beginning of the rev ORF was investigated. Deletion or attenuation of the Kozak sequence resulted in expression of an N-terminal 11  aa-truncated Rev in addition to WT Rev. Truncated Rev displayed weaker promotion of Gag expression and processing than WT Rev. Furthermore, EIAV rescued from an infectious molecular clone (pEIAVUK3) with Kozak attenuation exhibited decreased viral replication in host cells in vitro. These results provide a new understanding of the relationship between EIAV Rev expression and viral replication.

  1. Altered Sleep Homeostasis in Rev-erbα Knockout Mice

    PubMed Central

    Mang, Géraldine M.; La Spada, Francesco; Emmenegger, Yann; Chappuis, Sylvie; Ripperger, Jürgen A.; Albrecht, Urs; Franken, Paul

    2016-01-01

    Study Objectives: The nuclear receptor REV-ERBα is a potent, constitutive transcriptional repressor critical for the regulation of key circadian and metabolic genes. Recently, REV-ERBα's involvement in learning, neurogenesis, mood, and dopamine turnover was demonstrated suggesting a specific role in central nervous system functioning. We have previously shown that the brain expression of several core clock genes, including Rev-erbα, is modulated by sleep loss. We here test the consequences of a loss of REV-ERBα on the homeostatic regulation of sleep. Methods: EEG/EMG signals were recorded in Rev-erbα knockout (KO) mice and their wild type (WT) littermates during baseline, sleep deprivation, and recovery. Cortical gene expression measurements after sleep deprivation were contrasted to baseline. Results: Although baseline sleep/wake duration was remarkably similar, KO mice showed an advance of the sleep/wake distribution relative to the light-dark cycle. After sleep onset in baseline and after sleep deprivation, both EEG delta power (1–4 Hz) and sleep consolidation were reduced in KO mice indicating a slower increase of homeostatic sleep need during wakefulness. This slower increase might relate to the smaller increase in theta and gamma power observed in the waking EEG prior to sleep onset under both conditions. Indeed, the increased theta activity during wakefulness predicted delta power in subsequent NREM sleep. Lack of Rev-erbα increased Bmal1, Npas2, Clock, and Fabp7 expression, confirming the direct regulation of these genes by REV-ERBα also in the brain. Conclusions: Our results add further proof to the notion that clock genes are involved in sleep homeostasis. Because accumulating evidence directly links REV-ERBα to dopamine signaling the altered homeostatic regulation of sleep reported here are discussed in that context. Citation: Mang GM, La Spada F, Emmenegger Y, Chappuis S, Ripperger JA, Albrecht U, Franken P. Altered sleep homeostasis in Rev

  2. Hybrid offshore structure

    SciTech Connect

    Finn, L.D.; Maus, L.D.

    1986-09-09

    An articulated offshore structure is described for use in a body of water, the structure comprising: a substantially rigid lower section, the lower section extending upwardly from the bottom of the body of water to a pivot point located intermediate the bottom and the surface of the body of water; a compliant upper section extending upwardly from the pivot point to a position at or above the surface of the body of water; pivot means located proximate pivot point, the pivot means interposed between and connected to the lower section and upper section and adapted to permit the upper section to pivot laterally relative to the lower section; torsion means connected to the upper section and the lower section, the torsion means adapted to transmit torsional loads from the upper section to the lower section; the pivot means being positioned above the bottom of the body of water a distance of between about 10 percent and about 50 percent of the total depth of the body of water so as to substantially minimize the weight of the structure while maintaining the flexural vibration period of the structure at or below a preselected maximum flexural vibration period.

  3. Offshore well support miniplatform

    SciTech Connect

    Blandford, J.W.

    1987-07-14

    This patent describes a protective well support system installed on an offshore well having an upstanding conductor pipe extending above the seabed, the system comprising: (a) an upstanding conductor clamp (adapts to encircle the conductor pipe) formed by two upstanding facing members supported by a bottom engaging frame; (b) the frame including frame members joined together defining a substantially horizontal rectangular support frame for engaging the seabed; (c) angularly extending brace members secured at one end to the conductor clamp and at the other end to the frame; (d) the conductor clamp attaches to the frame and extending upright to enable the conductor clamp to fasten about the conductor pipe; (e) a boat landing mounts about the conductor pipe, the boat landing formed by at least two sectional components fixedly secured to semicircular clamp members adapted to encircle the conductor pipe for mounting the boat landing components; and (f) well platform means supported on bracket means adapted to encircle the conductor pipe for removably securing the well platform means.

  4. Reassessment of offshore platforms

    SciTech Connect

    Nair, V.V.D.; Kuhn, J.M. )

    1993-05-01

    Data from Hurricane Andrew demonstrated that the systems and procedures in place for evacuating offshore workers and minimizing oil spills and environmental damage functioned as planned. While the vast majority of the platforms survived the storm with no damage, a few of the older platforms (installed prior to 1972) either collapsed or suffered severe damage. The collapsed platforms were designed with insufficient deck height to clear the storm waves. In recent years, the API RP 2A has introduced guidance for minimum air gap, minimum structures, platform inspection and platform reuse. These provisions, coupled with natural attribution of the older platforms, will significantly improve the performance of platforms in the future. The reliability of NDT techniques to detect major structural defects should be improved through continued research. While flooded member detection is used by several operators as a screening tool to detect major defects underwater, its reliability is not always good and further research is needed in this area. Another area of high priority research is related to the use of Remotely Operated Vehicles (ROV) to perform underwater inspection of structures. 51 refs., 7 figs.

  5. Family Bovidae (Hollow-horned Ruminants)

    USGS Publications Warehouse

    Groves, Colin P.; Leslie, David M.; Huffman, Brent A.; Valdez, Raul; Habibi, Khushal; Weinberg, Paul; Burton, James; Jarman, Peter; Robichaud, William

    2015-01-01

    Probably the single most eye-catching aspect of the current volume is the explosion of species recognized in the family Bovidae (Hollow-horned Ruminants). In 2005, the third edition of Mammal Species of the World listed 143 species in 50 genera of Bovidae. That list, prepared by the late Peter Grubb, was somewhat traditional and provisional, as he was engaged with his long-time colleague, Colin Groves, in a substantial revision of ungulate taxonomy. Their work, which will be published later this year, is the culmination of years of study of this important and wide-ranging family by these two venerable authorities. Colin Groves is the lead author for Bovidae in this volume of HMW, and in it we recognize all 279 species in 54 genera that are documented in his and Peter Grubb’s ground-breaking work.At the root of this expanded number of recognized species is our changing view of the modern species concept. Like a growing number of taxonomists, Groves favors a phylogenetic species concept, which he defines as the smallest population or aggregation of populations that has fixed heritable differences from other such populations or aggregations. This is in contrast to the traditional biological species concept, which requires reproductive isolation between such populations. The difficulty in determining that reproductive isolation led to an underrepresentation of the number of species in many groups. Clearly there remain problems in determining which differences between populations are heritable, and the system used here undoubtedly will continue to be tweaked as our understanding grows. For now, this greatly expanded version of Bovidae species limits seems the best answer. One of the goals of HMW is to provide an up-to-date summary of the conservation status for every species of mammal, and this expanded species concept better enables us to explore the true conservation situation of each.

  6. Binding and thermodynamics of REV peptide-ctDNA interaction.

    PubMed

    Upadhyay, Santosh Kumar

    2017-03-01

    The thermodynamics of DNA-ligand binding is important as it provides useful information to understand the details of binding processes. HIV-1 REV response element (RRE) located in the env coding region of the viral genome is reported to be well conserved across different HIV-1 isolates. In this study, the binding characteristics of Calf thymus DNA (ctDNA) and REV peptide from HIV-1 were investigated using spectroscopic (UV-visible, fluorescence, and circular dichroism (CD)) and isothermal titration calorimetric (ITC) techniques. Thermal stability and ligand binding properties of the ctDNA revealed that native ctDNA had a Tm of 75.5 °C, whereas the ctDNA-REV peptide complex exhibited an incremental shift in the Tm by 8 °C, indicating thermal stability of the complex. CD data indicated increased ellipticity due to large conformational changes in ctDNA molecule upon binding with REV peptide and two binding stoichiometric modes are apparent. The ctDNA experienced condensation due to large conformational changes in the presence of REV peptide and positive B→Ψ transition was observed at higher molar charge ratios. Fluorescence studies performed at several ligand concentrations revealed a gradual decrease in the fluorescence intensity of EtBr-bound ctDNA in response to increasing ligand concentrations. The fluorescence data further confirmed two stoichiometric modes of binding for ctDNA-REV peptide complex as previously observed with CD studies. The binding enthalpies were determined using ITC in the temperature range of 293 K-308 K. The ITC binding isotherm was exothermic at all temperatures examined, with low ΔH values indicating that the ctDNA-REV peptide interaction is driven largely by entropy. The heat capacity change (ΔCp ) was insignificant, an unusual finding in the area of DNA-peptide interaction studies. The variation in the values obtained for ΔH, ΔS, and ΔG with temperature further suggests that ctDNA-REV peptide interaction is entropically

  7. Effects of measurement dependence on generalized Clauser-Horne-Shimony-Holt Bell test in the single-run and multiple-run scenarios

    NASA Astrophysics Data System (ADS)

    Li, Dan-Dan; Zhou, Yu-Qian; Gao, Fei; Li, Xin-Hui; Wen, Qiao-Yan

    2016-07-01

    Bell tests, as primitive tools to detect nonlocality in bipartite systems, rely on an assumption, i.e., measurement independence. Since ensuring measurement independence in a practical Bell test is difficult, it is crucial to explore the effects of relaxing this assumption. Recently, in the simplest Clauser-Horne-Shimony-Holt Bell (CHSH-Bell) test, Koh et al. [Phys. Rev. Lett. 109, 160404 (2012), 10.1103/PhysRevLett.109.160404] built the relation among measurement dependence, guessing probability, and the maximum value of CHSH-Bell correlation function that an adversary (Eve) can fake. As well, Pope et al. [Phys. Rev. A 88, 032110 (2013), 10.1103/PhysRevA.88.032110] and Yuan et al. [Phys. Rev. A. 91, 032111 (2015), 10.1103/PhysRevA.91.032111] settled the same problem in the multiple-run scenario with the general input distribution and the factorizable one, respectively. However, pertinent results in the generalized CHSH-Bell test are still missing. Here, we study this problem and establish the relation among measurement dependence, guessing probability, and the maximum value of the generalized CHSH-Bell correlation function that Eve can fake. Furthermore, we also consider the multiple-run scenario and show the relations in both input distributions. Interestingly, compared with the simplest CHSH-Bell test, we find that it is more difficult for Eve to fake a violation in the generalized CHSH-Bell test in some special cases. We expect that our conclusions will serve as a reference for quantum information processing tasks such as quantum key distribution, randomness expansion, and other tasks in the device-independent framework.

  8. Millimeter and Submillimeter-Wave Integrated Horn Antenna Schottky Receivers.

    NASA Astrophysics Data System (ADS)

    Ali-Ahmad, Walid Youssef

    1993-01-01

    Fundamental Schottky-diode mixers are currently used in most millimeter-wave receivers above 100GHz. The mixers use either a whisker-contacted diode or a planar Schottky diode suspended in a machined waveguide with an appropriate RF matching network. However, waveguide mounts are very expensive to machine for frequencies above 200GHz. Also, the whisker-contacted structure is not compatible with integrated mixers which represent the leading technology used for millimeter- and submillimeter-wave applications such as plasma diagnostics imaging arrays, radiometers, and anti-collision radars. In this work, a novel quasi-integrated horn antenna has been used for the receiver antenna. This antenna has a high gain and a high Gaussian coupling efficiency (97%), similar to machined scalar feed horns, but with the advantage of being easily fabricated up to at least 1.5THz. The quasi-integrated horn antenna is based on the integrated horn antenna structure. The integrated horn antenna consists of a pyramidal cavity with a 70^circ flare angle etched anisotropically in silicon. The cavity focuses the incoming energy on dipole-probe suspended on a membrane inside the horn. The integrated horn antenna does not suffer from dielectric losses or substrate mode losses since the feeding dipole antenna is integrated on a very thin dielectric layer. The mixer circuit, along with the feed dipole, are both integrated on the membrane wafer. The mixer diode is the University of Virginia surface channel planar diode which has a low parasitic capacitance. The diode is epoxied directly at the dipole apex without the need for an RF matching network, and with no mixer tuning required. At 92GHz,the DSB antenna-mixer conversion loss and noise temperature are 5.5dB and 770K, respectively. This represents the best reported results to this date for a quasi-optical mixer with a planar diode, at room temperature. At 335GHz, the DSB antenna-mixer noise temperature is 1750K and it is within 1dB of the

  9. Frontal horn thin walled cysts in preterm neonates are benign

    PubMed Central

    Pal, B; Preston, P; Morgan, M; Rushton, D; Durbin, G

    2001-01-01

    BACKGROUND—Screening cranial ultrasound led to the discovery of isolated frontal horn cysts quite distinct from periventricular leucomalacia cysts.
AIM—To clarify their significance, incidence, characteristics, causal factors or aetiology, and effect on long term outcome.
DESIGN—A retrospective observational study of all first cranial ultrasound scans (total of 2914) performed during the period 1984-1994 inclusive found 21 neonates with smooth thin walled frontal horn cysts: 18 of 2629 scanned were of birth weight < 1500 g or gestation < 33 weeks, and three of 285 were > 33 weeks gestation. Sequential ultrasound, maternal records, and neonatal events were retrospectively assessed. In survivors, routine neurodevelopmental evaluations were obtained. Postmortem studies of one cyst were performed to determine the nature and origin of these lesions.
RESULTS—Of the 21 subjects, 15 had isolated frontal horn cysts and six had additional ultrasound scan abnormalities, including four with subependymal haemorrhage. The sonographic features of frontal horn cysts were of distinctive morphology (elliptical, smooth, thin walled, ranging in size from 3 to 20 mm) and position (adjacent to the tip of the anterior horns). The cysts enlarged and then regressed by a median corrected age of 2 months. Subjects of < 33 weeks gestation (n = 18) had a median birth weight of 1465g (range 720-1990) and median gestation of 30 weeks (range 24-32). There was no consistent perinatal course. The neurodevelopmental outcome in 10 of the 11 survivors with isolated frontal horn cysts was normal. Five subjects died from causes unrelated to brain pathology in the neonatal period, and one subject died after infancy. Histological examination of a cyst at autopsy in one additional subject subsequent to the period of study confirmed the cyst to be lined by neuroblasts and ependymal cells.
CONCLUSIONS—The incidence of frontal horn cysts in this low birthweight population was 7 per 1000 (0

  10. Modeling of Horns for Sonic/Ultrasonic Applications

    NASA Technical Reports Server (NTRS)

    Sherrit, Stewart; Dolgin, Ben P.; Bar-Cohen, Yoseph

    2000-01-01

    JPL has a requirement for telerobotic tools for planetary sample acquisition, which require low power and have the ability to work in harsh environments. We are currently investigating the possibility of using ultrasonic horns to develop a family of ultrasonic tools for these environments. In an effort to determine control parameters a one-dimensional Mason's model for a stepped ultrasonic horn assembly was developed which includes the effects of mechanical and electrical losses in the piezoelectric material and acoustic elements. The model is separated into three regions; the piezoelectric stack including stress bolt the backing layer and the horn. The model is found to predict the impedance data of the horn assembly very accurately up to the first coupled (radial) resonance. The model also allows for the calculation of the velocity and force and power delivered to each acoustic element. FEM modeling and accelerometer data from the horn tip were used to corroborate the model. The difficulties associated with modeling the load impedance of various devices will be discussed and current directions noted.

  11. Tensile forces at the porcine anterior meniscal horn attachment.

    PubMed

    Stärke, Christian; Kopf, Sebastian; Gröbel, Karl-Heinz; Becker, Roland

    2009-12-01

    Tibiofemoral compression causes circumferential tension in the knee meniscus, which is transferred to the tibial bone at the anterior and posterior attachments. The objective of the study was to measure the resulting tensile forces at the horn attachment in a porcine model. The anterior horn attachment of the porcine medial meniscus (n = 10) was separated from the surrounding bone with a core reamer. A force transducer was installed such that tensile forces acting upon the now mobile horn attachment could be measured. The tibiofemoral joint was loaded in compression, starting at a preload of 30 N, with three 150-N increments, giving 180, 330, and 480 N load. Flexion angles of 0, 30, and 60 degrees were investigated. The average resultant tension at the horn attachment was 26.3, 40.6, and 55.4 N with full extension, 29.2, 47.8, and 62.2 N at 30 degrees flexion and 30.1, 49.6, and 68.1 N at 60 degrees flexion. The tibiofemoral compression had a significant effect on the tension (p < 0.001), whereas no influence of the flexion angle was found (p = 0.291). The study demonstrates that tibiofemoral compressive loads cause considerable tensile forces at the anterior meniscal horn attachment. The data are of interest for models of the repair or replacement of the knee menisci.

  12. Design and Characterization of a Lens TEM Horn

    NASA Astrophysics Data System (ADS)

    Bigelow, W. S.; Farr, E. G.; Bowen, L. H.; Ellibee, D. E.; Lawry, D. I.

    We investigate an approach for obtaining smaller UWB antennas with lower sidelobes than those offered by current designs. In support of this, we built and tested a lens TEM horn employing a collimating polyethylene aperture lens. It was thought that the lens TEM horn, with a more uniformly illuminated aperture field, might lead to higher gain with lower sidelobes than a comparably sized reflector IRA. That hypothesis is tested here. The lens horn, with a 30-cm aperture, has a maximum realized gain on boresight of 23 dB at 10 GHz. The normalized antenna impulse response is a clean peak with a full-width-at-half-maximum (FWHM) of 35 ps. For comparison, a highly optimized reflector IRA with 46-cm diameter achieves a maximum realized gain of 28 dB at 19 GHz and an impulse response with a FWHM of 30 ps. From theory, we expected the lens horn to exhibit lower sidelobes than the IRA. However, we did not observe that behavior in our experimental model. We suggest refinements which might lead to reduced sidelobes and increased gain in lens TEM horns.

  13. Offshore Wind Energy Market Overview (Presentation)

    SciTech Connect

    Baring-Gould, I.

    2013-07-01

    This presentation describes the current international market conditions regarding offshore wind, including the breakdown of installation costs, how to reduce costs, and the physical siting considerations considered when planning offshore wind construction. The presentation offers several examples of international existing and planned offshore wind farm sites and compares existing international offshore resources with U.S. resources. The presentation covers future offshore wind trends and cites some challenges that the United States must overcome before it will be able to fully develop offshore wind sites.

  14. Transverse electromagnetic horn antenna with resistively-loaded exterior surfaces

    DOEpatents

    Aurand, John F.

    1999-01-01

    An improved transverse electromagnetic (TEM) horn antenna comprises a resistive loading material on the exterior surfaces of the antenna plates. The resistive loading material attenuates or inhibits currents on the exterior surfaces of the TEM horn antenna. The exterior electromagnetic fields are of opposite polarity in comparison to the primary and desired interior electromagnetic field, thus inherently cause partial cancellation of the interior wave upon radiation or upon reception. Reducing the exterior fields increases the radiation efficiency of the antenna by reducing the cancellation of the primary interior field (supported by the interior surface currents). This increases the transmit gain and receive sensitivity of the TEM horn antenna, as well as improving the transient (time-domain) response.

  15. Pattern measurements of a low-sidelobe horn antenna

    NASA Technical Reports Server (NTRS)

    Janssen, M. A.; Bednarczyk, S. M.; Gulkis, S.; Marlin, H. W.; Smoot, G. F.

    1979-01-01

    The techniques and results of measuring the power pattern of a corrugated horn antenna designed for low sidelobes to levels 90 dB below the main beam maximum on both the E- and H- planes are presented. The study was motivated by a requirement for an antenna with very low side lobes to measure properties of the cosmic microwave background radiation from a satellite. The particular application calls for horn antennas of approximately 7 deg beamwidth, operating at discrete frequencies in the range of 20-90 GHz. It is demonstrated that the side lobes of horn antennas can be measured to significantly lower levels than has been achieved previously. Finally, it is noted that an improvement could be achieved by allowing for longer signal integrations and incorporating a switched reference measurement scheme to enable the accurate determination of very low signal levels.

  16. Assembly techniques used in construction of neutrino horns at BNL

    SciTech Connect

    Sims, W.P.; Carroll, A.; Leonhardt, W.; Monaghan, R.; Pearson, C.; Pendzick, A.; Ryan, G.; Sandberg, J.; Smith, G.; Stillman, P.

    1987-01-01

    This paper will describe the techniques used in the assembly of the neutrino focusing horns which were installed in the fast extracted beam at the Alternating Gradient Synchrotron. The horns are coaxial magnetic lenses that are pulsed to a maximum of 14 kV and 300 kA. The materials and techniques used are further complicated by the fact that the horn must survive in a high radiation environment. We will describe both the techniques and materials that were used in making the high current electrical connections. This will include the silver plating of aluminum, methods for handling thermal expansion and large magnetic forces, and the fabrication of rigid coaxial conductors. The techniques described in this paper can be used in the assembly of any pulsed high current device.

  17. Detecting flaws in Portland cement concrete using TEM horn antennae

    NASA Astrophysics Data System (ADS)

    Al-Qadi, Imad L.; Riad, Sedki M.; Su, Wansheng; Haddad, Rami H.

    1996-11-01

    To understand the dielectric properties of PCC and better correlate them with type and severity of PCC internal defects, a study was conducted to evaluate PCC complex permittivity and magnetic permeability over a wideband of frequencies using both time domain and frequency domain techniques. Three measuring devices were designed and fabricated: a parallel plate capacitor, a coaxial transmission line, and transverse electromagnetic (TEM) horn antennae. The TEM horn antenna covers the microwave frequencies. The measurement technique involves a time domain setup that was verified by a frequency domain measurement. Portland cement concrete slabs, 60 by 75 by 14 cm, were cast; defects include delamination, delamination filled with water, segregation, and chloride contamination. In this paper, measurements using the TEM horn antennae and the feasibility of detecting flaws at microwave frequency are presented.

  18. TSAR modeling of a TEM horn and surrounding structure

    SciTech Connect

    Ng, W.C.; Pennock, S.T.

    1993-11-01

    Modeling of a TEM horn was performed with the TSAR FDTD electromagnetics code. The modeling was done in stages, beginning with the simplest case, the bare antenna itself. Complexity was added in the form of a dielectric lens, an enclosing cylinder, a layer of absorber inside the cylinder, resistive terminations between the horn and cylinder, and a flat plate over all, electrically separate from the cylinder. The final configuration included all of the above, plus a ring of ferrite inside the cylinder, just ahead of the horn. Predictions of the far-field were made at roughly ten degree increments, more closely spaced near boresight, in both vertical and horizontal planes, through the antenna`s centerline. Time histories at those points were evaluated, and from the time histories power densities were calculated. Both time histories and power densities will be presented for the configurations modeled.

  19. Pattern measurements of a low-sidelobe horn antenna

    NASA Technical Reports Server (NTRS)

    Janssen, M. A.; Bednarczyk, S. M.; Gulkis, S.; Marlin, H. W.; Smoot, G. F.

    1979-01-01

    The techniques and results of measuring the power pattern of a corrugated horn antenna designed for low sidelobes to levels 90 dB below the main beam maximum on both the E- and H- planes are presented. The study was motivated by a requirement for an antenna with very low side lobes to measure properties of the cosmic microwave background radiation from a satellite. The particular application calls for horn antennas of approximately 7 deg beamwidth, operating at discrete frequencies in the range of 20-90 GHz. It is demonstrated that the side lobes of horn antennas can be measured to significantly lower levels than has been achieved previously. Finally, it is noted that an improvement could be achieved by allowing for longer signal integrations and incorporating a switched reference measurement scheme to enable the accurate determination of very low signal levels.

  20. Greenberger-Horne-Zeilinger state protocols for fully connected qubit networks

    SciTech Connect

    Galiautdinov, Andrei; Coffey, Mark W.; Deiotte, Ron

    2009-12-15

    We generalize the recently proposed Greenberger-Horne-Zeilinger tripartite protocol [A. Galiautdinov and J. M. Martinis, Phys. Rev. A 78, 010305(R) (2008)] to fully connected networks of weakly coupled qubits interacting by way of anisotropic Heisenberg exchange g(XX+YY)+g-tildeZZ. Our model differs from the more familiar Ising-Heisenberg chain in that here every qubit interacts with every other qubit in the circuit. The assumption of identical couplings on all qubit pairs allows an elegant proof of the protocol for arbitrary N. In order to further make contact with experiment, we study fidelity degradation due to coupling imperfections by numerically simulating the N=3 and 4 cases. Our simulations indicate that the best fidelity at unequal couplings is achieved when (a) the system is initially prepared in the uniform superposition state (similarly to how it is done in the ideal case) and (b) the entangling time and the final rotations on each of the qubits are appropriately adjusted.

  1. A new proof of tractability for ORD-horn relations

    SciTech Connect

    Ligozat, G.

    1996-12-31

    This paper gives an elementary proof of the tractability of a sub-class of temporal relations in Allen`s algebra and related temporal calculi, the class of pre-convex relations. In Allen`s case, this subclass coincides with the class of ORD-Horn relations. Nebel and Burckert defined ORD-Horn relations and proved that path-consistency is a sufficient condition for consistency of a network for this sub-class. We prove a stronger result: for each path-consistent network in the sub-class, we give an effective method for constructing a feasible scenario without backtrack.

  2. Coupling and degenerating modes in longitudinal-torsional step horns.

    PubMed

    Harkness, Patrick; Lucas, Margaret; Cardoni, Andrea

    2012-12-01

    Longitudinal-torsional vibration is used and proposed for a variety of ultrasonic applications including motors, welding, and rock-cutting. To obtain this behavior in an ultrasonic step horn one can either, (i) couple the longitudinal and torsional modes of the horn by incorporating a ring of diagonal slits in the thick base section or, (ii) place helical flutes in the thin stem section to degenerate the longitudinal mode into a modified behavior with a longitudinal-torsional motion. This paper compares the efficacy of these two design approaches using both numerical and experimental techniques. Copyright © 2012 Elsevier B.V. All rights reserved.

  3. Inclusion body disease in a great horned owl.

    PubMed

    Sileo, L; Carlson, H C; Crumley, S C

    1975-01-01

    The carcass of a great horned owl (Bubo virginianus), which had been found moribund in southern Ontario, was presented for necropsy. Throughout the liver and spleen were numerous white foci 1-2 mm in diameter; also noted were white plaques in the mucosae of the pharyngeal papillae and intestine. Results of light and electron microscopic studies and experimental transmission to two captive great horned owls suggested that this was a herpvirus disease similar and possibly indentical to the owl disease reported by other workers in Wiconsin and Australia.

  4. Offshore Energy Knowledge Exchange Workshop Report

    SciTech Connect

    none,

    2012-04-12

    A report detailing the presentations and topics discussed at the Offshore Energy Knowledge Exchange Workshop, an event designed to bring together offshore energy industry representatives to share information, best practices, and lessons learned.

  5. 78 FR 27913 - Revision of Crane Regulation Standards for Mobile Offshore Drilling Units (MODUs), Offshore...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-05-13

    ... for Mobile Offshore Drilling Units (MODUs), Offshore Supply Vessels (OSVs), and Floating Outer... testing of cranes. These regulations apply to cranes installed on Mobile Offshore Drilling Units (MODUs... Maritime Organization MODU Mobile offshore drilling unit NFPA National Fire Protection Association NPRM...

  6. Designs and Development of Multimode Horns for ASTRO-G/VSOP-2 Satellite

    NASA Astrophysics Data System (ADS)

    Ujihara, H.

    2009-08-01

    The antenna optics of VSOP-2 satellite require low cross polarization, and the volume of the receiver box is limited. Thus, instead of conventional corrugated horns, multimode horns were proposed and designed in order to reduce the axial length and weight of the horns but still compatible with a low cross polarization. These multimode horns were designed for three observational bands of VSOP-2 at 8GHz, 22GHz, 43GHz, all with about the same antenna illumination size. However, the ratio of waveguide and wavelength are slightly different. The 22GHz-horn was designed at first, and the other horns were arranged around it. The properties of the horns were improved by controlling the complex amplitude of higher modes and by fitting the beam width to the antenna optics. The BBM models of horns were tested, and their measured beam patterns agree well with numerical simulations.

  7. Development drilling problems offshore Malaysia

    SciTech Connect

    Osman, M.N.; Maung, M.

    1984-02-01

    This paper highlights the major drilling problems encountered in the field development programmes offshore Malaysia from 1978 to mid-1983. The major problems identified were pipe sticking which was common in all the areas and top hole drilling problems encountered in a few fields offshore Sabah and Sarawak. Generally, the problems were related to drilling deviated wells through the soft and unconsolidated formations common in this region. Preventive measures employed by the Contractors have been effective in overcoming these problems in the later years of the period under consideration.

  8. Offshore wind farm layout optimization

    NASA Astrophysics Data System (ADS)

    Elkinton, Christopher Neil

    Offshore wind energy technology is maturing in Europe and is poised to make a significant contribution to the U.S. energy production portfolio. Building on the knowledge the wind industry has gained to date, this dissertation investigates the influences of different site conditions on offshore wind farm micrositing---the layout of individual turbines within the boundaries of a wind farm. For offshore wind farms, these conditions include, among others, the wind and wave climates, water depths, and soil conditions at the site. An analysis tool has been developed that is capable of estimating the cost of energy (COE) from offshore wind farms. For this analysis, the COE has been divided into several modeled components: major costs (e.g. turbines, electrical interconnection, maintenance, etc.), energy production, and energy losses. By treating these component models as functions of site-dependent parameters, the analysis tool can investigate the influence of these parameters on the COE. Some parameters result in simultaneous increases of both energy and cost. In these cases, the analysis tool was used to determine the value of the parameter that yielded the lowest COE and, thus, the best balance of cost and energy. The models have been validated and generally compare favorably with existing offshore wind farm data. The analysis technique was then paired with optimization algorithms to form a tool with which to design offshore wind farm layouts for which the COE was minimized. Greedy heuristic and genetic optimization algorithms have been tuned and implemented. The use of these two algorithms in series has been shown to produce the best, most consistent solutions. The influences of site conditions on the COE have been studied further by applying the analysis and optimization tools to the initial design of a small offshore wind farm near the town of Hull, Massachusetts. The results of an initial full-site analysis and optimization were used to constrain the boundaries of

  9. Eighteenth annual offshore technology conference. Volume 1

    SciTech Connect

    Not Available

    1986-01-01

    These sixty papers were given at a conference on offshore technology. Topics covered include friction effects of driving piles into sea beds of various compositions, wave forces on offshore platforms, stability, materials testing of various components such as plates, legs, wellheads, pipe joints, and protection of offshore platforms against ice and collision with icebergs.

  10. 49 CFR 222.21 - When must a locomotive horn be used?

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... section or shall sound the locomotive horn in the manner required by State law, or in the absence of State... locomotives traveling at speeds in excess of 60 mph shall not begin sounding the horn more than one-quarter... where railroads did not sound the horn, as of December 18, 2003. (d) Trains, locomotive consists and...

  11. 49 CFR 222.21 - When must a locomotive horn be used?

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... section or shall sound the locomotive horn in the manner required by State law, or in the absence of State... locomotives traveling at speeds in excess of 60 mph shall not begin sounding the horn more than one-quarter... where railroads did not sound the horn, as of December 18, 2003. (d) Trains, locomotive consists and...

  12. 9 CFR 95.12 - Bones, horns, and hoofs; importations permitted subject to restrictions.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 9 Animals and Animal Products 1 2012-01-01 2012-01-01 false Bones, horns, and hoofs; importations... ENTRY INTO THE UNITED STATES § 95.12 Bones, horns, and hoofs; importations permitted subject to restrictions. Bones, horns, and hoofs offered for importation which do not meet the conditions or requirements...

  13. 9 CFR 95.11 - Bones, horns, and hoofs for trophies or museums; disinfected hoofs.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 9 Animals and Animal Products 1 2010-01-01 2010-01-01 false Bones, horns, and hoofs for trophies..., OFFERED FOR ENTRY INTO THE UNITED STATES § 95.11 Bones, horns, and hoofs for trophies or museums; disinfected hoofs. (a) Clean, dry bones, horns, and hoofs, that are free from undried pieces of hide, flesh...

  14. 9 CFR 95.12 - Bones, horns, and hoofs; importations permitted subject to restrictions.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 9 Animals and Animal Products 1 2010-01-01 2010-01-01 false Bones, horns, and hoofs; importations... ENTRY INTO THE UNITED STATES § 95.12 Bones, horns, and hoofs; importations permitted subject to restrictions. Bones, horns, and hoofs offered for importation which do not meet the conditions or requirements...

  15. 9 CFR 95.12 - Bones, horns, and hoofs; importations permitted subject to restrictions.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 9 Animals and Animal Products 1 2013-01-01 2013-01-01 false Bones, horns, and hoofs; importations... ENTRY INTO THE UNITED STATES § 95.12 Bones, horns, and hoofs; importations permitted subject to restrictions. Bones, horns, and hoofs offered for importation which do not meet the conditions or requirements...

  16. 9 CFR 95.12 - Bones, horns, and hoofs; importations permitted subject to restrictions.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 9 Animals and Animal Products 1 2014-01-01 2014-01-01 false Bones, horns, and hoofs; importations... ENTRY INTO THE UNITED STATES § 95.12 Bones, horns, and hoofs; importations permitted subject to restrictions. Bones, horns, and hoofs offered for importation which do not meet the conditions or requirements...

  17. 9 CFR 95.11 - Bones, horns, and hoofs for trophies or museums; disinfected hoofs.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 9 Animals and Animal Products 1 2013-01-01 2013-01-01 false Bones, horns, and hoofs for trophies..., OFFERED FOR ENTRY INTO THE UNITED STATES § 95.11 Bones, horns, and hoofs for trophies or museums; disinfected hoofs. (a) Clean, dry bones, horns, and hoofs, that are free from undried pieces of hide, flesh...

  18. 9 CFR 95.11 - Bones, horns, and hoofs for trophies or museums; disinfected hoofs.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 9 Animals and Animal Products 1 2011-01-01 2011-01-01 false Bones, horns, and hoofs for trophies..., OFFERED FOR ENTRY INTO THE UNITED STATES § 95.11 Bones, horns, and hoofs for trophies or museums; disinfected hoofs. (a) Clean, dry bones, horns, and hoofs, that are free from undried pieces of hide, flesh...

  19. 9 CFR 95.12 - Bones, horns, and hoofs; importations permitted subject to restrictions.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 9 Animals and Animal Products 1 2011-01-01 2011-01-01 false Bones, horns, and hoofs; importations... ENTRY INTO THE UNITED STATES § 95.12 Bones, horns, and hoofs; importations permitted subject to restrictions. Bones, horns, and hoofs offered for importation which do not meet the conditions or requirements...

  20. 9 CFR 95.11 - Bones, horns, and hoofs for trophies or museums; disinfected hoofs.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 9 Animals and Animal Products 1 2014-01-01 2014-01-01 false Bones, horns, and hoofs for trophies..., OFFERED FOR ENTRY INTO THE UNITED STATES § 95.11 Bones, horns, and hoofs for trophies or museums; disinfected hoofs. (a) Clean, dry bones, horns, and hoofs, that are free from undried pieces of hide, flesh...

  1. 9 CFR 95.11 - Bones, horns, and hoofs for trophies or museums; disinfected hoofs.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 9 Animals and Animal Products 1 2012-01-01 2012-01-01 false Bones, horns, and hoofs for trophies..., OFFERED FOR ENTRY INTO THE UNITED STATES § 95.11 Bones, horns, and hoofs for trophies or museums; disinfected hoofs. (a) Clean, dry bones, horns, and hoofs, that are free from undried pieces of hide, flesh...

  2. 49 CFR 222.59 - When may a wayside horn be used?

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 49 Transportation 4 2010-10-01 2010-10-01 false When may a wayside horn be used? 222.59 Section... ADMINISTRATION, DEPARTMENT OF TRANSPORTATION USE OF LOCOMOTIVE HORNS AT PUBLIC HIGHWAY-RAIL GRADE CROSSINGS... part may be used in lieu of a locomotive horn at any highway-rail grade crossing equipped with...

  3. 49 CFR 222.59 - When may a wayside horn be used?

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 49 Transportation 4 2014-10-01 2014-10-01 false When may a wayside horn be used? 222.59 Section... ADMINISTRATION, DEPARTMENT OF TRANSPORTATION USE OF LOCOMOTIVE HORNS AT PUBLIC HIGHWAY-RAIL GRADE CROSSINGS... part may be used in lieu of a locomotive horn at any highway-rail grade crossing equipped with an...

  4. 49 CFR 222.59 - When may a wayside horn be used?

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 49 Transportation 4 2011-10-01 2011-10-01 false When may a wayside horn be used? 222.59 Section 222.59 Transportation Other Regulations Relating to Transportation (Continued) FEDERAL RAILROAD... Exceptions to the Use of the Locomotive Horn Silenced Horns at Groups of Crossings-Quiet Zones § 222.59...

  5. 49 CFR 222.21 - When must a locomotive horn be used?

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 49 Transportation 4 2011-10-01 2011-10-01 false When must a locomotive horn be used? 222.21 Section 222.21 Transportation Other Regulations Relating to Transportation (Continued) FEDERAL RAILROAD... of Locomotive Horns § 222.21 When must a locomotive horn be used? (a) Except as provided in this...

  6. 49 CFR 222.21 - When must a locomotive horn be used?

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 49 Transportation 4 2013-10-01 2013-10-01 false When must a locomotive horn be used? 222.21 Section 222.21 Transportation Other Regulations Relating to Transportation (Continued) FEDERAL RAILROAD... of Locomotive Horns § 222.21 When must a locomotive horn be used? (a) Except as provided in this...

  7. An Engaged Pragmatist: Uncovering and Assessing Ernest Horn's View of Moral Education

    ERIC Educational Resources Information Center

    Schul, James E.; Hamot, Gregory E.

    2011-01-01

    Ernest Horn was a curriculum professor at The University of Iowa in the early to mid-twentieth century. Predominantly known at the time for his spelling research, Horn also made important contributions to the field of social studies education. This historical inquiry illuminates one of Horn's contributions to social studies education by examining…

  8. Foundations for offshore wind turbines.

    PubMed

    Byrne, B W; Houlsby, G T

    2003-12-15

    An important engineering challenge of today, and a vital one for the future, is to develop and harvest alternative sources of energy. This is a firm priority in the UK, with the government setting a target of 10% of electricity from renewable sources by 2010. A component central to this commitment will be to harvest electrical power from the vast energy reserves offshore, through wind turbines or current or wave power generators. The most mature of these technologies is that of wind, as much technology transfer can be gained from onshore experience. Onshore wind farms, although supplying 'green energy', tend to provoke some objections on aesthetic grounds. These objections can be countered by locating the turbines offshore, where it will also be possible to install larger capacity turbines, thus maximizing the potential of each wind farm location. This paper explores some civil-engineering problems encountered for offshore wind turbines. A critical component is the connection of the structure to the ground, and in particular how the load applied to the structure is transferred safely to the surrounding soil. We review previous work on the design of offshore foundations, and then present some simple design calculations for sizing foundations and structures appropriate to the wind-turbine problem. We examine the deficiencies in the current design approaches, and the research currently under way to overcome these deficiencies. Designs must be improved so that these alternative energy sources can compete economically with traditional energy suppliers.

  9. China offshore has top priority

    SciTech Connect

    Cornitius, T.A.

    1983-11-01

    Joint venture development of offshore petroleum reserves has top priority in the mammoth effort to modernize the People's Republic of China. While the country works to overcome a critical shortage of skilled personnel, export of oil, coal, consumer goods, and metals will finance technological imports.

  10. The offshore benthic fish community

    USGS Publications Warehouse

    Lantry, Brian F.; Lantry, Jana R.; Weidel, Brian C.; Walsh, Maureen; Hoyle, James A.; Schaner, Teodore; Neave, Fraser B.; Keir, Michael

    2014-01-01

    The offshore benthic fish community will be composed of self-sustaining native fishes characterized by lake trout as the top predator, a population expansion of lake whitefish from northeastern waters to other areas of the lake, and rehabilitated native prey fishes.

  11. Proceedings of Offshore Europe 91

    SciTech Connect

    Not Available

    1991-01-01

    All papers in this volume were presented at Offshore Europe held in Aberdeen, Scotland, September 3--6, 1991. Included are the following papers: Low-damage-fluid-loss control for well completions; Isolation techniques for subsea gas pipelines; New water injection technology.

  12. Non-territorial floaters in Great Horned Owls (Bubo virginianus)

    Treesearch

    Christoph Rohner

    1997-01-01

    The ecology and behavior of non-territorial owls are basically unknown. I studied the integration of young Great Horned Owls (Bubo virginianus) into the territorial breeding population from 1988-1993 in the southwestern Yukon, Canada, during a peak and decline of the population cycle of snowshoe hares (Lepus americanus). Fifty-five...

  13. Morphological character of crystalline components present in saiga horn.

    PubMed

    Hashiguchi, K; Hashimoto, K; Akao, M

    2001-05-01

    The purpose of this study was to investigate the ultrastructure of saiga-antelope (Saiga tatarica) horn for proposing the mechanism of the initial mineralization. Horn is derived from horny tooth of Cyclostomata. The minerals in saiga horn were identified crystallographically using electron microscopy and X-ray diffraction techniques. Soft X-ray photographs revealed the degree of the mineralization pattern. However, the number of rings did not indicate the age of saiga. Mineral deposites were observed among well banded keratin fibers and composed of powder like crystals. This deposited crystals were found by the X-ray diffraction method to be octacalcium phospate (OCP) by comparing these periodic lattice fringes to JCPDS card data. The chemical formula of OCP is Ca8H2(PO4)6.5H2O. Evidences for the presence of OCP in mature hard tissues have never been obtained. This phenomenon described here may be characteristic of saiga horn because we have found no reports on this type of OCP mineralization in any other animal species. It is possible that OCP is the precursor in the initial mineralization step, indicating in a nucleation of mineral on the keratin fibers.

  14. Relationship between horn fly infestation and polymorphisms in cytochrome

    USDA-ARS?s Scientific Manuscript database

    Individual animal variation occurs regarding external parasite infestation in beef cattle. Our objective was to determine if horn flies infestations present on beef cattle are associated with the single nucleotide polymorphism (SNP; T-318C) in the cytochrome P450 gene (CYP3A28) and the prolactin (PR...

  15. Vegetative endocarditis in a scimitar-horned oryx (Oryx dammah).

    PubMed

    Chai, N

    1999-12-01

    Streptococcus uberis was cultured from vegetative endocarditis lesions in a scimitar-horned oryx (Oryx dammah) from the Parc de la Haute Touche, France. This is the first reported single isolation of S. uberis from an oryx with vegetative endocarditis leading to fatal congestive heart failure.

  16. Bioassay improvements for assessing pyrethroid resistance in horn flies

    USDA-ARS?s Scientific Manuscript database

    The horn fly (Haematobia irritans) is a major cattle pest in the American continent, causing yearly economic losses of over US $860 million in Brazil. Frequent use of chemical insecticides has led to the development of insecticide resistance, leading to significant economic losses and environmental ...

  17. Project Echo: Horn-Reflector Antenna for Space Communication

    NASA Technical Reports Server (NTRS)

    Crawford, A. B.; Hogg, D. C.; Hunt, L. E.

    1961-01-01

    This paper describes the mechanical features of the horn- reflector antenna used for receiving signals reflected from the Project Echo balloon satellite, and presents in some detail the electrical characteristics (radiation patterns and gain) measured at a frequency of 2390 Mc. Theoretically derived characteristics which agree very well with the measurements are also presented; details of the calculations are given in the appendices.

  18. Past and future rainfall in the Horn of Africa

    PubMed Central

    Tierney, Jessica E.; Ummenhofer, Caroline C.; deMenocal, Peter B.

    2015-01-01

    The recent decline in Horn of Africa rainfall during the March–May “long rains” season has fomented drought and famine, threatening food security in an already vulnerable region. Some attribute this decline to anthropogenic forcing, whereas others maintain that it is a feature of internal climate variability. We show that the rate of drying in the Horn of Africa during the 20th century is unusual in the context of the last 2000 years, is synchronous with recent global and regional warming, and therefore may have an anthropogenic component. In contrast to 20th century drying, climate models predict that the Horn of Africa will become wetter as global temperatures rise. The projected increase in rainfall mainly occurs during the September–November “short rains” season, in response to large-scale weakening of the Walker circulation. Most of the models overestimate short rains precipitation while underestimating long rains precipitation, causing the Walker circulation response to unrealistically dominate the annual mean. Our results highlight the need for accurate simulation of the seasonal cycle and an improved understanding of the dynamics of the long rains season to predict future rainfall in the Horn of Africa. PMID:26601306

  19. Synovial chondromatosis in a great horned owl (Bubo virginianus).

    PubMed

    Howard, M O; Nieves, M A; Miles, K G

    1996-04-01

    A case of synovial chondromatosis in a great horned owl (Bubo virginianus) was found in June 1993. In radiographs of bilateral swelling of the scapulohumeral joint we observed numerous mineralized foci in the soft tissue. The foci were identified by light microscopy as cartilaginous metaplasia. This is the first report of synovial chondromatosis in an owl.

  20. Ventral horn cell responses to spaceflight and hindlimb suspension

    NASA Technical Reports Server (NTRS)

    Jiang, Bian; Roy, Roland R.; Poliakov, I. V.; Krasnov, I. B.; Edgerton, V. R.

    1992-01-01

    Ventral horn cells of the lumbosacral enlargement of the spinal cord were studied focusing on the succinate dehydrogenase (SDH) activity and cross-sectional area of the soma of ventral horn cells which were measured using a computer-aided image-processing system. The relationships between the soma size and SDH activities of lumber ventral horn cells after 14 days of spaceflight (Cosmos 2044) or of hindlimb suspension are considered. Three groups of rats under consideration include control, 14-day spaceflight, and 14-day hindlimb suspension. Data obtained indicate that, compared to the control group, the population distribution of SDH activities in the flight rats shifted toward higher activities, whereas in the suspended rats the distribution shifted toward lower activities. The interactive effects within individual cells showed that there was a higher percentage of small cells with high SDH activities in the flight than in the control or suspended rats. It is suggested that changes in ventral horn cells were due to factors other than simply the absence of weight support.

  1. Lower corner of Face B Array with near field horn, ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    Lower corner of Face B Array with near field horn, foreground left, looking north/northwest - Beale Air Force Base, Perimeter Acquisition Vehicle Entry Phased-Array Warning System, Techinical Equipment Building, End of Spencer Paul Road, north of Warren Shingle Road (14th Street), Marysville, Yuba County, CA

  2. Crosscurrents of Criticism: Horn Book Essays 1968-1977.

    ERIC Educational Resources Information Center

    Heins, Paul, Ed.

    More than forty articles originally published in "The Horn Book Magazine" between 1968 and 1977 are grouped into eleven topical sections in this volume. The sections deal with the status, classification, and evaluation of children's literature; conflicting standards on which children's books are judged; fantasy, humor, and current trends in…

  3. Dog-Bone Horns for Piezoelectric Ultrasonic/Sonic Actuators

    NASA Technical Reports Server (NTRS)

    Sherrit, Stewart; Bar-Cohen, Yoseph; Chang, Zensheu; Bao, Xiaoqi

    2007-01-01

    A shape reminiscent of a dog bone has been found to be superior to other shapes for mechanical-amplification horns that are components of piezoelectrically driven actuators used in a series of related devices denoted generally as ultrasonic/sonic drill/corers (USDCs). The first of these devices was reported in Ultrasonic/Sonic Drill/Corers With Integrated Sensors (NPO-20856), NASA Tech Briefs, Vol. 25, No. 1 (January 2001), page 38. The dog-bone shape was conceived especially for use in a more recent device in the series, denoted an ultrasonic/ sonic gopher, that was described in Ultrasonic/Sonic Mechanisms for Drilling and Coring (NPO-30291), NASA Tech Briefs, Vol. 27, No. 9 (September 2003), page 65. The figure shows an example of a dog-bone-shaped horn and other components of an ultrasonic gopher. Prerequisite to a meaningful description of this development is an unavoidably lengthy recapitulation of the principle of operation of a USDC and, more specifically, of the ultrasonic/sonic gopher as described previously in NASA Tech Briefs. The ultrasonic actuator includes a stack of piezoelectric rings, the horn, a metal backing, and a bolt that connects the aforementioned parts and provides compressive pre-strain to the piezoelectric stack to prevent breakage of the rings during extension. The stack of piezoelectric rings is excited at the resonance frequency of the overall ultrasonic actuator. Through mechanical amplification by the horn, the displacement in the ultrasonic vibration reaches tens of microns at the tip of the horn. The horn hammers an object that is denoted the free mass because it is free to move longitudinally over a limited distance between hard stops: The free mass bounces back and forth between the ultrasonic horn and a tool bit (a drill bit or a corer). Because the longitudinal speed of the free mass is smaller than the longitudinal speed of vibration of the tip of the horn, contact between the free mass and the horn tip usually occurs at a

  4. REV-ERB and ROR: therapeutic targets for treating myopathies

    NASA Astrophysics Data System (ADS)

    Welch, Ryan D.; Flaveny, Colin A.

    2017-08-01

    Muscle is primarily known for its mechanical roles in locomotion, maintenance of posture, and regulation of cardiac and respiratory function. There are numerous medical conditions that adversely affect muscle, myopathies that disrupt muscle development, regeneration and protein turnover to detrimental effect. Skeletal muscle is also a vital secretory organ that regulates thermogenesis, inflammatory signaling and directs context specific global metabolic changes in energy substrate preference on a daily basis. Myopathies differ in the causative factors that drive them but share common features including severe reduction in quality of life and significantly increased mortality all due irrefutably to the loss of muscle mass. Thus far clinically viable approaches for preserving muscle proteins and stimulating new muscle growth without unwanted side effects or limited efficacy has been elusive. Over the last few decades, evidence has emerged through in vitro and in vivo studies that suggest the nuclear receptors REV-ERB and ROR might modulate pathways involved in myogenesis and mitochondrial biogenesis. Hinting that REV-ERB and ROR might be targeted to treat myopathies. However there is still a need for substantial investigation into the roles of these nuclear receptors in in vivo rodent models of degenerative muscle diseases and acute injury. Although exciting, REV-ERB and ROR have somewhat confounding roles in muscle physiology and therefore more studies utilizing in vivo models of skeletal muscle myopathies are needed. In this review we highlight the molecular forces driving some of the major degenerative muscular diseases and showcase two promising molecular targets that may have the potential to treat myopathies: ROR and REV-ERB.

  5. National Offshore Wind Energy Grid Interconnection Study

    SciTech Connect

    Daniel, John P.; Liu, Shu; Ibanez, Eduardo; Pennock, Ken; Reed, Greg; Hanes, Spencer

    2014-07-30

    The National Offshore Wind Energy Grid Interconnection Study (NOWEGIS) considers the availability and potential impacts of interconnecting large amounts of offshore wind energy into the transmission system of the lower 48 contiguous United States. A total of 54GW of offshore wind was assumed to be the target for the analyses conducted. A variety of issues are considered including: the anticipated staging of offshore wind; the offshore wind resource availability; offshore wind energy power production profiles; offshore wind variability; present and potential technologies for collection and delivery of offshore wind energy to the onshore grid; potential impacts to existing utility systems most likely to receive large amounts of offshore wind; and regulatory influences on offshore wind development. The technologies considered the reliability of various high-voltage ac (HVAC) and high-voltage dc (HVDC) technology options and configurations. The utility system impacts of GW-scale integration of offshore wind are considered from an operational steady-state perspective and from a regional and national production cost perspective.

  6. The role of doublesex in the evolution of exaggerated horns in the Japanese rhinoceros beetle

    PubMed Central

    Ito, Yuta; Harigai, Ayane; Nakata, Moe; Hosoya, Tadatsugu; Araya, Kunio; Oba, Yuichi; Ito, Akinori; Ohde, Takahiro; Yaginuma, Toshinobu; Niimi, Teruyuki

    2013-01-01

    Male-specific exaggerated horns are an evolutionary novelty and have diverged rapidly via intrasexual selection. Here, we investigated the function of the conserved sex-determination gene doublesex (dsx) in the Japanese rhinoceros beetle (Trypoxylus dichotomus) using RNA interference (RNAi). Our results show that the sex-specific T. dichotomus dsx isoforms have an antagonistic function for head horn formation and only the male isoform has a role for thoracic horn formation. These results indicate that the novel sex-specific regulation of dsx during horn morphogenesis might have been the key evolutionary developmental event at the transition from sexually monomorphic to sexually dimorphic horns. PMID:23609854

  7. Nuclear Export Signal Masking Regulates HIV-1 Rev Trafficking and Viral RNA Nuclear Export.

    PubMed

    Behrens, Ryan T; Aligeti, Mounavya; Pocock, Ginger M; Higgins, Christina A; Sherer, Nathan M

    2017-02-01

    HIV-1's Rev protein forms a homo-oligomeric adaptor complex linking viral RNAs to the cellular CRM1/Ran-GTP nuclear export machinery through the activity of Rev's prototypical leucine-rich nuclear export signal (NES). In this study, we used a functional fluorescently tagged Rev fusion protein as a platform to study the effects of modulating Rev NES identity, number, position, or strength on Rev subcellular trafficking, viral RNA nuclear export, and infectious virion production. We found that Rev activity was remarkably tolerant of diverse NES sequences, including supraphysiological NES (SNES) peptides that otherwise arrest CRM1 transport complexes at nuclear pores. Rev's ability to tolerate a SNES was both position and multimerization dependent, an observation consistent with a model wherein Rev self-association acts to transiently mask the NES peptide(s), thereby biasing Rev's trafficking into the nucleus. Combined imaging and functional assays also indicated that NES masking underpins Rev's well-known tendency to accumulate at the nucleolus, as well as Rev's capacity to activate optimal levels of late viral gene expression. We propose that Rev multimerization and NES masking regulates Rev's trafficking to and retention within the nucleus even prior to RNA binding.

  8. Study of disbudding goat kids following injection of clove oil essence in horn bud region.

    PubMed

    Molaei, Mohammad Mahdi; Mostafavi, Ali; Kheirandish, Reza; Azari, Omid; Shaddel, Mohsen

    2015-01-01

    This study was performed to evaluate the efficacy of injection of essential oil of Eugenia caryophyllata in the kid horn buds, as a new chemical technique for disbudding. Five-day-old healthy goat kids from both sexes (n = 16) were divided randomly into 4 equal groups. In groups 1, 2 and 3, 0.2 mL of clove essence and in group 4 (control) 0.2 mL of normal saline was injected into the left horn bud of goat kids. Right horn bud in all kids was considered to ensure that they are horned. During the study, the rate of horn growth were evaluated in determined time intervals between groups 1 and 4. Tissue samples were taken from right and left horn bud in groups 2 and 3, at five and ten days after clove essence injection, for microscopic study. The results of the study showed that the clove essence stopped horn growth, whereas there was no significant difference in horn growth rate between left and right horns after injection of normal saline, in group 4. Histopathological study showed that injection of clove essence caused complete necrosis of epidermis and underlying dermis with collagenolysis in horn bud tissues, 5 days after injection and then progress in healing process was observed after 10 days. According to the results of this study, it can be concluded that the injection of clove essence is an effective method to stop horn growth without any undesirable effects on clinical parameters in goat kids.

  9. Changes in horn size of Stone's sheep over four decades correlate with trophy hunting pressure.

    PubMed

    Douhard, Mathieu; Festa-Bianchet, Marco; Pelletier, Fanie; Gaillard, Jean-michel; Bonenfanti, Christophe

    2016-01-01

    Selective harvest may lead to rapid evolutionary change. For large herbivores, trophy hunting removes males with large horns. That artificial selection, operating in opposition to sexual selection, can lead to undesirable consequences for management and conservation. There have been no comparisons of long-term changes in trophy size under contrasting harvest pressures. We analyzed horn measurements of Stone's rams (Ovis dalli stonei) harvested over 37 years in two large regions of British Columbia, Canada, with marked differences in hunting pressure to identify when selective hunting may cause a long-term decrease in horn growth. Under strong selective harvest, horn growth early in life and the number of males harvested declined by 12% and 45%, respectively, over the study period. Horn shape also changed over time: horn length became shorter for a given base circumference, likely because horn base is not a direct target of hunter selection. In contrast, under relatively lower hunting pressure, there were no detectable temporal trends in early horn growth, number of males harvested, or horn length relative to base circumference. Trophy hunting is an important recreational activity and can generate substantial revenues for conservation. By providing a reproductive advantage to males with smaller horns and reducing the availability of desirable trophies, however, excessive harvest may have the undesirable long-term consequences of reducing both the harvest and the horn size of rams. These consequences can be avoided by limiting offtake.

  10. A planar Bézier profiled horn for reducing penetration force in ultrasonic cutting.

    PubMed

    Wang, Dung-An; Nguyen, Hai-Dang

    2014-01-01

    An ultrasonic planar horn with a Bézier profile is developed. The first longitudinal displacement mode of the horn is exploited for high displacement amplification in order to reduce the penetration force required to enter and cut materials. The displacement amplification and stress distribution characteristics of the Bézier horn and the commonly used catenary horn are examined. The penetration force by the Bézier horn is nearly 75% of that by the catenary horn with a penetration speed of 0.25 mm/s during cutting a tissue stimulant. At a penetration speed of 0.5 mm/s, the penetration force by the Bézier horn is nearly 85% of that by the catenary horn for cutting a polymethylmethacrylate (PMMA) material. The decrease in the penetration force by the Bézier horn is attributed to the fact that the displacement amplification of the Bézier horn is 30% higher than that of the traditional catenary horn with the same length and end surface widths. Copyright © 2013 Elsevier B.V. All rights reserved.

  11. Micro-Horn Arrays for Ultrasonic Impedance Matching

    NASA Technical Reports Server (NTRS)

    Rao, Shanti; Palmer, Dean

    2009-01-01

    Thin-layered structures containing arrays of micromachined horns, denoted solid micro-horn arrays (SMIHAs), have been conceived as improved means of matching acoustic impedances between ultrasonic transducers and the media with which the transducers are required to exchange acoustic energy. Typically, ultrasonic transducers (e.g., those used in medical imaging) are piezoelectric or similar devices, which produce small displacements at large stresses. However, larger displacements at smaller stresses are required in the target media (e.g., human tissues) with which acoustic energy is to be exchanged. Heretofore, efficiencies in transmission of acoustic energy between ultrasonic transducers and target media have been severely limited because substantial mismatches of acoustic impedances have remained, even when coupling material layers have been interposed between the transducers and the target media. In contrast, SMIHAs can, in principle, be designed to effect more nearly complete acoustic impedance matching, leading to power transmission efficiencies of 90 percent or even greater. The SMIHA concept is based on extension, into the higher-frequency/ lower-wavelength ultrasonic range, of the use of horns to match acoustic impedances in the audible and lower-frequency ultrasonic ranges. In matching acoustic impedance in transmission from a higher-impedance acoustic source (e.g., a piezoelectric transducer) and a lowerimpedance target medium (e.g., air or human tissue), a horn acts as a mechanical amplifier. The shape and size of the horn can be optimized for matching acoustic impedance in a specified frequency range. A typical SMIHA would consist of a base plate, a face plate, and an array of horns that would constitute pillars that connect the two plates (see figure). In use, the base plate would be connected to an ultrasonic transducer and the face plate would be placed in contact with the target medium. As at lower frequencies, the sizes and shapes of the pillars

  12. Age-dependent relationship between horn growth and survival in wild sheep.

    PubMed

    Bonenfant, Christophe; Pelletier, Fanie; Garel, Mathieu; Bergeron, Patrick

    2009-01-01

    1. Trade-offs in resource allocation underline the evolution of life-history traits but their expression is frequently challenged by empirical findings. In large herbivores, males with large antlers or horns typically have high mating success. The fitness costs of large horns or antlers have rarely been quantified although they are controversial. 2. Here, using detailed longitudinal data on n = 172 bighorn (Ovis canadensis, Shaw) and the capture-mark-recapture methodology, we tested whether early horn growth leads to a survival cost in rams ('trade-off' hypothesis) or if males that can afford rapid horn growth survive better than males of lower phenotypic quality ('phenotypic quality' hypothesis). We also quantified how hunting increased survival costs of bearing large horns. 3. We found an age-specific relationship between horn growth and survival. In all age classes, natural survival was either weakly related to (lambs, adult rams) or positively associated (yearling rams) with early horn growth. Hunting mortality was markedly different from natural mortality of bighorn rams, leading to an artificial negative association between early horn growth and survival. Beginning at age 4, the yearly harvest rate ranged from 12% for males with the smallest horns up to more than 40% for males with the largest horns. 4. Growing large horns early in life is not related to any consistent survival costs, hence supporting the phenotypic quality hypothesis in males of a dimorphic and polygynous large herbivores. Rapid horn growth early in life is, however, strongly counter selected by trophy hunting. We suggest that horn size is a very poor index of reproductive effort and that males modulate their mating activities and energy allocation to horn growth to limit its impact on survival.

  13. User Instructions for the Systems Assessment Capability, Rev. 1, Computer Codes Volume 3: Utility Codes

    SciTech Connect

    Eslinger, Paul W.; Aaberg, Rosanne L.; Lopresti, Charles A.; Miley, Terri B.; Nichols, William E.; Strenge, Dennis L.

    2004-09-14

    This document contains detailed user instructions for a suite of utility codes developed for Rev. 1 of the Systems Assessment Capability. The suite of computer codes for Rev. 1 of Systems Assessment Capability performs many functions.

  14. Biology and control of tabanids, stable flies and horn flies.

    PubMed

    Foil, L D; Hogsette, J A

    1994-12-01

    Tabanids are among the most free-living adult flies which play a role as livestock pests. A single blood meal is used as a source of energy for egg production (100-1,000 eggs per meal), and females of certain species can oviposit before a blood meal is obtained (autogeny). Therefore, the maintenance of annual populations requires successful oviposition by only 2% of females. Wild animal blood sources are usually available to maintain annual tabanid populations. Larval habitats are also independent of domestic livestock. Thus, the use of repellents or partial repellents is the only effective chemical strategy to reduce the incidence of tabanids on livestock. Permanent traps (and possibly treated silhouette traps) can be employed to intercept flies. Selective grazing or confinement can also reduce the impact of tabanids. Stable fly adults are dependent on vertebrate blood for survival and reproduction, but the amount of time spent in contact with the host is relatively small. Stable fly larvae develop in manure, spilled feed and decaying vegetation. Management of larval habitats by sanitation is the key to stable fly control. Treatment of animals with residual insecticides can aid in control; thorough application to the lower body parts of livestock is important. Proper use of modified traps, using either treated targets or solar-powered electrocution grids, can be effective in reducing stable fly populations. Adult horn flies spend the major part of their time on the host, and the larvae are confined to bovid manure. Therefore, almost any form of topical insecticide application for livestock is effective against horn flies, in the absence of insecticide resistance. Treatments should be applied when economic benefit is possible; economic gains are associated with increased weaning weights and weight gains of yearling and growing cattle. Oral chemical treatments (insect growth regulators or insecticides) administered at appropriate rates via bolus, water, food or

  15. Attached cavitation at a small diameter ultrasonic horn tip

    NASA Astrophysics Data System (ADS)

    Žnidarčič, Anton; Mettin, Robert; Cairós, Carlos; Dular, Matevž

    2014-02-01

    Ultrasonic horn transducers are frequently used in applications of acoustic cavitation in liquids, for instance, for cell disruption or sonochemical reactions. They are operated typically in the frequency range up to about 50 kHz and have tip diameters from some mm to several cm. It has been observed that if the horn tip is sufficiently small and driven at high amplitude, cavitation is very strong, and the tip can be covered entirely by the gas/vapor phase for longer time intervals. A peculiar dynamics of the attached cavity can emerge with expansion and collapse at a self-generated frequency in the subharmonic range, i.e., below the acoustic driving frequency. Here, we present a systematic study of the cavitation dynamics in water at a 20 kHz horn tip of 3 mm diameter. The system was investigated by high-speed imaging with simultaneous recording of the acoustic emissions. Measurements were performed under variation of acoustic power, air saturation, viscosity, surface tension, and temperature of the liquid. Our findings show that the liquid properties play no significant role in the dynamics of the attached cavitation at the small ultrasonic horn. Also the variation of the experimental geometry, within a certain range, did not change the dynamics. We believe that the main two reasons for the peculiar dynamics of cavitation on a small ultrasonic horn are the higher energy density on a small tip and the inability of the big tip to "wash" away the gaseous bubbles. Calculation of the somewhat adapted Strouhal number revealed that, similar to the hydrodynamic cavitation, values which are relatively low characterize slow cavitation structure dynamics. In cases where the cavitation follows the driving frequency this value lies much higher - probably at Str > 20. In the spirit to distinguish the observed phenomenon with other cavitation dynamics at ultrasonic transducer surfaces, we suggest to term the observed phenomenon of attached cavities partly covering the full horn

  16. Nuclear Export Signal Masking Regulates HIV-1 Rev Trafficking and Viral RNA Nuclear Export

    PubMed Central

    Behrens, Ryan T.; Aligeti, Mounavya; Pocock, Ginger M.; Higgins, Christina A.

    2016-01-01

    ABSTRACT HIV-1's Rev protein forms a homo-oligomeric adaptor complex linking viral RNAs to the cellular CRM1/Ran-GTP nuclear export machinery through the activity of Rev's prototypical leucine-rich nuclear export signal (NES). In this study, we used a functional fluorescently tagged Rev fusion protein as a platform to study the effects of modulating Rev NES identity, number, position, or strength on Rev subcellular trafficking, viral RNA nuclear export, and infectious virion production. We found that Rev activity was remarkably tolerant of diverse NES sequences, including supraphysiological NES (SNES) peptides that otherwise arrest CRM1 transport complexes at nuclear pores. Rev's ability to tolerate a SNES was both position and multimerization dependent, an observation consistent with a model wherein Rev self-association acts to transiently mask the NES peptide(s), thereby biasing Rev's trafficking into the nucleus. Combined imaging and functional assays also indicated that NES masking underpins Rev's well-known tendency to accumulate at the nucleolus, as well as Rev's capacity to activate optimal levels of late viral gene expression. We propose that Rev multimerization and NES masking regulates Rev's trafficking to and retention within the nucleus even prior to RNA binding. IMPORTANCE HIV-1 infects more than 34 million people worldwide causing >1 million deaths per year. Infectious virion production is activated by the essential viral Rev protein that mediates nuclear export of intron-bearing late-stage viral mRNAs. Rev's shuttling into and out of the nucleus is regulated by the antagonistic activities of both a peptide-encoded N-terminal nuclear localization signal and C-terminal nuclear export signal (NES). How Rev and related viral proteins balance strong import and export activities in order to achieve optimal levels of viral gene expression is incompletely understood. We provide evidence that multimerization provides a mechanism by which Rev transiently masks

  17. How dangerous is offshore drilling

    SciTech Connect

    McIntosh, T.S.

    1981-08-01

    As concluded by the National Research Council, the frequency of injuries during oil and gas operations on the outer continental shelf is comparable to that in other industries such as mining, maritime service, and heavy construction. Although an area of concern, offshore safety is not easily improved by legislation or detailed regulation. The industry itself must be committed to providing the safest possible workplace for the job and the environment. At Zapata Off-Shore Co., for example, top management recognizes the value of safety-trained personnel in terms of not only decreased downtime and lower insurance costs but also improved crew morale and productivity. The key operatives of Zapata's program are the rig representatives who provide full-time, rig-based safety, training, administrative, and employee-relations assistance to the crews.

  18. Offshore drilling platform protection device

    SciTech Connect

    Magill, J.M.

    1981-12-15

    A description is given of an offshore drilling platform protection device for use on an offshore oil well drilling vessel including a drilling platform supportable on a plurality of extendable legs wherein each leg is moved by a rack gear assembly. The rack gear assembly includes an otherwise exposed first gear which engages a second gear positioned in a housing having a rectangular corner opening through which the first gear extends, the protection device including first and second protective sections adapted for mounting over the first gear adjacent to the rectangular corner of the housing for the second gear, the first and second protective sections cooperating to provide an l-shaped opening which communicates with the opening at the second gear housing for protecting the first gear while allowing the first gear to mesh with the second gear.

  19. Fatigue handbook: Offshore steel structures

    SciTech Connect

    Almarnaess, A.

    1985-01-01

    The contents of this book are: Overview of Offshore Steel Structures; Loads on Ocean Structures; Fracture Mechanics As a Tool in Fatigue Analysis; Basic Fatigue Properties of Welded Joints; Significance of Defects; Improving the Fatigue Strength of Welded Joints; Effects of Marine Environment and Cathodic Protection on Fatigue of Structural Steels Fatigue of Tubular Joints; Unstable Fracture; Fatigue Life Calculations; and Fatigue in Building Codes Background and Applications.

  20. Pipelaying in artic offshore waters

    SciTech Connect

    Langner, C. G.

    1985-11-19

    The present invention provides a method and apparatus for constructing pipelines in Arctic offshore waters by a directional drilling technique, thereby minimizing exposure to ice gouging and eliminating the hazards associated with unstable permafrost. A special drilling-pipe-line construction vessel is also provided which has a conical shape with reinforced outer walls to resist ice forces, which vessel includes means to install deep underground pipeline segments and means to connect and protect the pipe ends.

  1. Two offshore Australian crudes assayed

    SciTech Connect

    Rhodes, A.K.

    1994-05-09

    Two light, sweet crudes from offshore Australia have been assayed. Gippsland crude, also called Bass Strait, is produced off the coast of Victoria, in southeastern Australia. The 47 API, 0.09% sulfur crude was analyzed in mid-1993. Skua, a 42 API, 0.06 wt % sulfur crude, is produced in the Timor Sea. Data are given on the whole crude and fractions for both deposits. Both chemical and physical properties are listed.

  2. Offshore sand and gravel mining

    SciTech Connect

    Pandan, J.W.

    1983-05-01

    This paper reviews the status of mining offshore for sand and gravel on a world-wide basis. It discusses the technology for exploration and evaluation of sea floor mineral targets, as well as mining, transportation, and processing. Large operations in Japan and Europe are described, based upon personal observations of the author. The U.S. situation is outlined and opinions offered as to the outlook for the future.

  3. Offshore oil: Correctness of perspective

    SciTech Connect

    Burns, R.F.

    1993-05-01

    Except for the Gulf of Mexico, the offshore oil industry has been virtually banned from the US Exclusive Economic Zone for ten years. The oil potential in Alaska's Arctic National Wildlife Refuge (ANWR) is also off limits. The Gulf of Mexico is the only place with prospects for future success and a number of companies both large and small are determined to move forward. The depressed price of oil does not encourage development but recently gas prices in the US have increased, making offshore gas development more feasible. Perhaps most significant is development and application of new technology and more intense management to make sure it works. The offshore oil companies and support industries have made significant technological advances, expending over and above the dollars paid in taxes, lease fees, and royalties. The ocean industries harbor a great reservoir of high technology knowledge. They have demonstrated the ability to successfully meet a vast array of challenges in exploring for, drilling, and producing oil and gas in extreme conditions. These facts beg the question as to the rational basis of each and every regulation and the ban on drilling.

  4. RNA helicase MOV10 functions as a co-factor of HIV-1 Rev to facilitate Rev/RRE-dependent nuclear export of viral mRNAs

    SciTech Connect

    Huang, Feng; Zhang, Junsong; Zhang, Yijun; Geng, Guannan; Liang, Juanran; Li, Yingniang; Chen, Jingliang; Liu, Chao; Zhang, Hui

    2015-12-15

    Human immunodeficiency virus type 1 (HIV-1) exploits multiple host factors during its replication. The REV/RRE-dependent nuclear export of unspliced/partially spliced viral transcripts needs the assistance of host proteins. Recent studies have shown that MOV10 overexpression inhibited HIV-1 replication at various steps. However, the endogenous MOV10 was required in certain step(s) of HIV-1 replication. In this report, we found that MOV10 potently enhances the nuclear export of viral mRNAs and subsequently increases the expression of Gag protein and other late products through affecting the Rev/RRE axis. The co-immunoprecipitation analysis indicated that MOV10 interacts with Rev in an RNA-independent manner. The DEAG-box of MOV10 was required for the enhancement of Rev/RRE-dependent nuclear export and the DEAG-box mutant showed a dominant-negative activity. Our data propose that HIV-1 utilizes the anti-viral factor MOV10 to function as a co-factor of Rev and demonstrate the complicated effects of MOV10 on HIV-1 life cycle. - Highlights: • MOV10 can function as a co-factor of HIV-1 Rev. • MOV10 facilitates Rev/RRE-dependent transport of viral mRNAs. • MOV10 interacts with Rev in an RNA-independent manner. • The DEAG-box of MOV10 is required for the enhancement of Rev/RRE-dependent export.

  5. Recognition of the high affinity binding site in rev-response element RNA by the human immunodeficiency virus type-1 rev protein.

    PubMed Central

    Iwai, S; Pritchard, C; Mann, D A; Karn, J; Gait, M J

    1992-01-01

    The Human Immunodeficiency Virus type-1 rev protein binds with high affinity to a bubble structure located within the rev-response element (RRE) RNA in stemloop II. After this initial interaction, additional rev molecules bind to the RRE RNA in an ordered assembly process which requires a functional bubble structure, since mutations in the bubble sequence that reduce rev affinity block multiple complex formation. We have used synthetic chemistry to characterize the interaction between rev protein and its high affinity binding site. A minimal synthetic duplex RNA (RBC6) carrying the bubble and 12 flanking base pairs is able to bind rev with 1 to 1 stoichiometry and with high affinity. When the bubble structure is inserted into synthetic RNA molecules carrying longer stretches of flanking double-stranded RNA, rev forms additional complexes resembling the multimers observed with the RRE RNA. The ability of rev to bind to RBC6 analogues containing functional group modifications on base and sugar moieties of nucleoside residues was also examined. The results provide strong evidence that the bubble structure contains specific configurations of non-Watson--Crick G:G and G:A base pairs and suggest that high affinity recognition of RRE RNA by rev requires hydrogen bonding to functional groups in the major groove of a distorted RNA structure. Images PMID:1282702

  6. Simplified horn antenna parameter estimation using selective criteria

    SciTech Connect

    Ewing, P.D.

    1991-01-01

    An approximation can be used to avoid the complex mathematics and computation methods typically required for calculating the gain and radiation pattern of electromagnetic horn antenna. Because of the curvature of the antenna wave front, calculations using conventional techniques involve solving the Fresnel integrals and using computer-aided numerical integration. With this model, linear approximations give a reasonable estimate of the gain and radiation pattern using simple trigonometric functions, thereby allowing a hand calculator to replace the computer. Applying selected criteria, the case of the E-plane horn antenna was used to evaluate this technique. Results showed that the gain approximation holds for an antenna flare angle of less than 10{degree} for typical antenna dimensions, and the E field radiation pattern approximation holds until the antenna's phase error approaches 60{degree}, both within typical design parameters. This technique is a useful engineering tool. 4 refs., 11 figs.

  7. Beam steering and impedance matching of plasmonic horn nanoantennas

    NASA Astrophysics Data System (ADS)

    Afridi, Adeel; Kocabaş, Şükrü Ekin

    2016-10-01

    In this paper, we study a plasmonic horn nanoantenna on a metal-backed substrate. The horn nanoantenna structure consists of a two-wire transmission line (TWTL) flared at the end. We analyze the effect of the substrate thickness on the nanoantenna's radiation pattern, and demonstrate beam steering in a broad range of elevation angles. Furthermore, we analyze the effect of the ground plane on the impedance matching between the antenna and the TWTL, and observe that the ground plane increases the back reflection into the waveguide. To reduce the reflection, we develop a transmission line model to design an impedance matching section which leads to 99.75% power transmission to the nanoantenna.

  8. Beam steering and impedance matching of plasmonic horn nanoantennas.

    PubMed

    Afridi, Adeel; Kocabaş, Şükrü Ekin

    2016-10-31

    In this paper, we study a plasmonic horn nanoantenna on a metal-backed substrate. The horn nanoantenna structure consists of a two-wire transmission line (TWTL) flared at the end. We analyze the effect of the substrate thickness on the nanoantenna's radiation pattern, and demonstrate beam steering in a broad range of elevation angles. Furthermore, we analyze the effect of the ground plane on the impedance matching between the antenna and the TWTL, and observe that the ground plane increases the back reflection into the waveguide. To reduce the reflection, we develop a transmission line model to design an impedance matching section which leads to 99.75% power transmission to the nanoantenna.

  9. General proof of the Greenberger-Horne-Zeilinger theorem

    SciTech Connect

    Chen Zeqian

    2004-09-01

    It is proved that all states of three spin-(1/2) particles exhibiting an 'all versus nothing' contradiction between quantum mechanics and the local realism of Einstein, Podolsky, and Rosen are exactly the Greenberger-Horne-Zeilinger (GHZ) states and the states obtained from them by local unitary transformations. The proof is obtained by showing that there are at most four elements (except for a different sign) in a set of mutually commuting nonlocal spin observables in the three-qubit system and using the certain algebraic properties that Pauli's matrices satisfy. We show that only does such a set of four nonlocal spin observables present a Greenberger-Horne-Zeilinger-Mermin-like argument. This also reveals the equivalence between the GHZ theorem and maximal violation of the Bell inequality.

  10. Simplified horn antenna parameter estimation using selective criteria

    NASA Astrophysics Data System (ADS)

    Ewing, P. D.

    1991-03-01

    An approximation can be used to avoid the complex mathematics and computation methods typically required for calculating the gain and radiation pattern of electromagnetic horn antenna. Because of the curvature of the antenna wave front, calculations using conventional techniques involve solving the Fresnel integrals and using computer-aided numerical integration. With this model, linear approximations give a reasonable estimate of the gain and radiation pattern using simple trigonometric functions, thereby allowing a hand calculator to replace the computer. Applying selected criteria, the case of the E-plane horn antenna was used to evaluate this technique. Results showed that the gain approximation holds for an antenna flare angle of less than 10 degrees for typical antenna dimensions, and the E field radiation pattern approximation holds until the antenna's phase error approaches 60 degrees, both within typical design parameters. This technique is a useful engineering tool.

  11. Spatial patterns in the abundance of the coastal horned lizard

    USGS Publications Warehouse

    Fisher, Robert N.; Suarez, Andrew V.; Case, Ted J.

    2002-01-01

    Coastal horned lizards (   Phrynosoma coronatum) have undergone severe declines in southern California and are a candidate species for state and federal listing under the Endangered Species Act. Quantitative data on their habitat use, abundance, and distribution are lacking, however. We investigated the determinants of abundance for coastal horned lizards at multiple spatial scales throughout southern California. Specifically, we estimated lizard distribution and abundance by establishing 256 pitfall trap arrays clustered within 21 sites across four counties. These arrays were sampled bimonthly for 2–3 years. At each array we measured 26 “local” site descriptors and averaged these values with other “regional” measures to determine site characteristics. Our analyses were successful at identifying factors within and among sites correlated with the presence and abundance of coastal horned lizards. These factors included the absence of the invasive Argentine ant (  Linepithema humile) (and presence of native ant species eaten by the lizards), the presence of chaparral community plants, and the presence of sandy substrates. At a regional scale the relative abundance of Argentine ants was correlated with the relative amount of developed edge around a site. There was no evidence for spatial autocorrelation, even at the scale of the arrays within sites, suggesting that the determinants of the presence or absence and abundance of horned lizard can vary over relatively small spatial scales ( hundreds of meters). Our results suggest that a gap-type approach may miss some of the fine-scale determinants of species abundance in fragmented habitats.

  12. Pattern measurements of a low-sidelobe horn antenna

    NASA Technical Reports Server (NTRS)

    Janssen, M. A.; Gulkis, S.; Bednarczyk, S. M.; Marlin, H. W.; Smoot, G. F.

    1980-01-01

    The techniques and results of power pattern measurements of a corrugated horn antenna designed for low sidelobes are reported. The power pattern was measured to levels 90 dB below the main beam maximum in both the E- and H-planes. The measured patterns were found to be in good agreement with predictions from existing theory for the performance of corrugated scalar feeds.

  13. Gene discovery in the horned beetle Onthophagus taurus

    PubMed Central

    2010-01-01

    Background Horned beetles, in particular in the genus Onthophagus, are important models for studies on sexual selection, biological radiations, the origin of novel traits, developmental plasticity, biocontrol, conservation, and forensic biology. Despite their growing prominence as models for studying both basic and applied questions in biology, little genomic or transcriptomic data are available for this genus. We used massively parallel pyrosequencing (Roche 454-FLX platform) to produce a comprehensive EST dataset for the horned beetle Onthophagus taurus. To maximize sequence diversity, we pooled RNA extracted from a normalized library encompassing diverse developmental stages and both sexes. Results We used 454 pyrosequencing to sequence ESTs from all post-embryonic stages of O. taurus. Approximately 1.36 million reads assembled into 50,080 non-redundant sequences encompassing a total of 26.5 Mbp. The non-redundant sequences match over half of the genes in Tribolium castaneum, the most closely related species with a sequenced genome. Analyses of Gene Ontology annotations and biochemical pathways indicate that the O. taurus sequences reflect a wide and representative sampling of biological functions and biochemical processes. An analysis of sequence polymorphisms revealed that SNP frequency was negatively related to overall expression level and the number of tissue types in which a given gene is expressed. The most variable genes were enriched for a limited number of GO annotations whereas the least variable genes were enriched for a wide range of GO terms directly related to fitness. Conclusions This study provides the first large-scale EST database for horned beetles, a much-needed resource for advancing the study of these organisms. Furthermore, we identified instances of gene duplications and alternative splicing, useful for future study of gene regulation, and a large number of SNP markers that could be used in population-genetic studies of O. taurus and

  14. Gene discovery in the horned beetle Onthophagus taurus.

    PubMed

    Choi, Jeong-Hyeon; Kijimoto, Teiya; Snell-Rood, Emilie; Tae, Hongseok; Yang, Youngik; Moczek, Armin P; Andrews, Justen

    2010-12-14

    Horned beetles, in particular in the genus Onthophagus, are important models for studies on sexual selection, biological radiations, the origin of novel traits, developmental plasticity, biocontrol, conservation, and forensic biology. Despite their growing prominence as models for studying both basic and applied questions in biology, little genomic or transcriptomic data are available for this genus. We used massively parallel pyrosequencing (Roche 454-FLX platform) to produce a comprehensive EST dataset for the horned beetle Onthophagus taurus. To maximize sequence diversity, we pooled RNA extracted from a normalized library encompassing diverse developmental stages and both sexes. We used 454 pyrosequencing to sequence ESTs from all post-embryonic stages of O. taurus. Approximately 1.36 million reads assembled into 50,080 non-redundant sequences encompassing a total of 26.5 Mbp. The non-redundant sequences match over half of the genes in Tribolium castaneum, the most closely related species with a sequenced genome. Analyses of Gene Ontology annotations and biochemical pathways indicate that the O. taurus sequences reflect a wide and representative sampling of biological functions and biochemical processes. An analysis of sequence polymorphisms revealed that SNP frequency was negatively related to overall expression level and the number of tissue types in which a given gene is expressed. The most variable genes were enriched for a limited number of GO annotations whereas the least variable genes were enriched for a wide range of GO terms directly related to fitness. This study provides the first large-scale EST database for horned beetles, a much-needed resource for advancing the study of these organisms. Furthermore, we identified instances of gene duplications and alternative splicing, useful for future study of gene regulation, and a large number of SNP markers that could be used in population-genetic studies of O. taurus and possibly other horned beetles.

  15. Endomorphins: localization, release and action on rat dorsal horn neurons.

    PubMed

    Dun, N J; Dun, S L; Wu, S Y; Williams, C A; Kwok, E H

    2000-01-01

    Endomorphin (Endo) 1 and 2, two tetrapeptides isolated from the bovine and human brain, have been proposed to be the endogenous ligand for the mu-opiate receptor. A multi-disciplinary study was undertaken to address the issues of localization, release and biological action of Endo with respect to the rat dorsal horn. First, immunohistochemical studies showed that Endo-1- or Endo-2-like immunoreactivity (Endo-1- or Endo-2-LI) is selectively expressed in fiber-like elements occupying the superficial layers of the rat dorsal horn, which also exhibit a high level of mu-opiate receptor immunoreactivity. Second, release of immunoreactive Endo-2-like substances (irEndo) from the in vitro rat spinal cords upon electrical stimulation of dorsal root afferent fibers was detected by the immobilized antibody microprobe technique. The site of release corresponded to laminae I and II where the highest density of Endo-2-LI fibers was localized. Lastly, whole-cell patch clamp recordings from substantia gelatinosa (SG) neurons of rat lumbar spinal cord slices revealed two distinct actions of exogenous Endo-1 and Endo-2: (1) depression of excitatory and/or inhibitory postsynaptic potentials evoked by stimulation of dorsal root entry zone, and (2) hyperpolarization of SG neurons. These two effects were prevented by the selective mu-opiate receptor antagonist beta-funaltrexamine. The localization of endomorphin-positive fibers in superficial layers of the dorsal horn and the release of irEndo upon stimulation of dorsal root afferents together with the observation that Endo inhibits the activity of SG neurons by interacting with mu-opiate receptors provide additional support of a role of Endo as the endogenous ligand for the mu-opiate receptor in the rat dorsal horn.

  16. Rehabilitation and water quality monitoring in the Golden Horn.

    PubMed

    Sur, H I; Okuş, E; Sarikay, H Z; Altiok, H; Eroğlu, V; Oztürk, I

    2002-01-01

    In this work, the oceanographic aspects of the Golden Horn and some results of the Golden Horn Rehabilitation Project are presented. The hydrographic structure of the Golden Horn responds rapidly to the conditions in the southern Bosphorus, which is especially true for the outer parts of the estuary up to the Valide Sultan Bridge (VS). West of this bridge which was blocked by the pontoons of the bridge, carries the major pollution load and is dependent on the underlying water and surface mixing, for its renewal. The dissolved oxygen concentrations were measured below the detection limit in the region between the VS and Eyüp-Sütlüce (ES) section during the majority of the measurement periods where H2S has been determined simultaneously until February 2000. Although the microbial contamination of the estuary stations is very high, decreases in the contamination at stations are observed. The highest concentrations are recorded at the ES-VS section and supported by Fecal Coliform data during the rainy months in general despite the operational collector system surrounding the estuary. An obvious decrease of pollution in comparison with the data of the previous years is clearly observed as an encouraging result of the rehabilitation efforts achieved so far.

  17. Occupational cow horn eye injuries in ibadan, Nigeria.

    PubMed

    Ibrahim, Oa; Olusanya, Ba

    2014-11-01

    This case series aims to describe the clinical features, management, and outcome of occupational eye injuries caused by cow horns and to recommend possible preventive measures. A review of patients with cow horn inflicted eye injuries seen at the University College Hospital, Ibadan between January 2006, and December 2011 was conducted. Three patients were identified, and their charts were reviewed for demographic information, mechanism of injury, initial and final visual acuity, surgeries performed, as well as anatomic and visual outcome. The three patients were males and were aged 45, 22, and 49 years, respectively. They were all involved in cattle-related jobs, and they all had unilateral open-globe injuries with corneoscleral lacerations. Presenting visual acuity was nil light perception in the injured eyes. The contralateral eyes were all normal. Two of the patients required evisceration of the eye, while the third patient underwent repair of lacerations. Visual improvement was not achieved in any of the patients. Cow horn eye injuries may be quite severe and could result in loss of the eye with monocular blindness. Careful attention must be paid to prevent such injuries. Cattle rearers and dealers should wear safety goggles, and proper restraint of the animals is advocated.

  18. Ultrasonic Heat Transfer Enhancement Using a Horn-Type Transducer

    NASA Astrophysics Data System (ADS)

    Nomura, Shinfuku; Yamamoto, Akira; Murakami, Koichi

    2002-05-01

    The purpose of this study is to clarify experimentally the influence of streaming induced by ultrasonic vibration on heat transfer using a horn-type ultrasonic vibrator. A horn tip of 6 mm diameter and 60.7 kHz resonant frequency was used as the ultrasonic transducer. Heat transfer experiments for a downward-facing horizontal heating surface with ultrasonic vibration from below were carried out in a natural convection region. The acoustic jet in the water from the horn tip of the transducer regarded as a nozzle exit was induced by this transducer, and as a result, up to a ten-fold increase in heat transfer coefficient was obtained by application of 20 W in both tap water and degassed water. It was found that the mechanism of heat transfer enhancement by ultrasonic vibration in tap water can be classified into four categories. In degassed water, heat transfer enhancement is influenced not by the acoustic jet, but by small-scale perturbations by cavitation microjets.

  19. Offshore and arctic frontiers -structures, ocean mining

    SciTech Connect

    Chung, J.S.

    1985-05-01

    The systematic development of offshore technology is discussed. Today, this technology enables the production of approximately 14 million barrels of oil per day, or 26% of oil production worldwide. The evolution in offshore structures is examined with emphasis on jacket and jackup platforms. Challenges are explored. Microprocessors, data-base management, and artificial intelligence are mentioned as having an impact on the offshore and arctic oil industry.

  20. Novel Features of the Prenatal Horn Bud Development in Cattle (Bos taurus).

    PubMed

    Wiener, Dominique Judith; Wiedemar, Natalie; Welle, Monika Maria; Drögemüller, Cord

    2015-01-01

    Whereas the genetic background of horn growth in cattle has been studied extensively, little is known about the morphological changes in the developing fetal horn bud. In this study we histologically analyzed the development of horn buds of bovine fetuses between ~70 and ~268 days of pregnancy and compared them with biopsies taken from the frontal skin of the same fetuses. In addition we compared the samples from the wild type (horned) fetuses with samples taken from the horn bud region of age-matched genetically hornless (polled) fetuses. In summary, the horn bud with multiple layers of vacuolated keratinocytes is histologically visible early in fetal life already at around day 70 of gestation and can be easily differentiated from the much thinner epidermis of the frontal skin. However, at the gestation day (gd) 212 the epidermis above the horn bud shows a similar morphology to the epidermis of the frontal skin and the outstanding layers of vacuolated keratinocytes have disappeared. Immature hair follicles are seen in the frontal skin at gd 115 whereas hair follicles below the horn bud are not present until gd 155. Interestingly, thick nerve bundles appear in the dermis below the horn bud at gd 115. These nerve fibers grow in size over time and are prominent shortly before birth. Prominent nerve bundles are not present in the frontal skin of wild type or in polled fetuses at any time, indicating that the horn bud is a very sensitive area. The samples from the horn bud region from polled fetuses are histologically equivalent to samples taken from the frontal skin in horned species. This is the first study that presents unique histological data on bovine prenatal horn bud differentiation at different developmental stages which creates knowledge for a better understanding of recent molecular findings.

  1. 1991 worldwide offshore contractors and equipment directory

    SciTech Connect

    Not Available

    1991-01-01

    This book is the information source-book for the international offshore oil industry. Within this single convenient reference you'll find addresses, phone numbers, telex, fax and cable listings for more than 3,500 companies and their key personnel in the drilling, workover, construction, service/supply/manufacturers, geophysical, diving and transportation segments of the offshore industry. Along with this vital contact information, the authors include such pertinent data as rig specifications, ownership, an equipment index, a company index and a current survey of offshore production systems from Offshore Incorporating the Oilman.

  2. Analysis of the virulence-associated RevSR two-component signal transduction system of Clostridium perfringens.

    PubMed

    Cheung, Jackie K; Wisniewski, Jessica A; Adams, Vicki M; Quinsey, Noelene S; Rood, Julian I

    2016-09-01

    Clostridium perfringens is a Gram-positive, anaerobic, spore-forming bacterium that causes human gas gangrene (clostridial myonecrosis) and food poisoning. Early studies showed that virulence was regulated by the VirSR two-component signal transduction system. However, our identification of the RevR orphan response regulator indicated that more than one system was involved in controlling virulence. To further characterize this virulence-associated regulator, gel mobility shift experiments, coupled with DNase I footprinting, were used to identify the RevR DNA binding sequence. Bioinformatics analysis suggested that an orphan sensor histidine kinase, CPE1757 (renamed RevS), was the cognate sensor of RevR. Interaction between RevS and RevR was demonstrated by use of a bacterial two-hybrid system and validated by protein-protein interaction studies using biolayer interferometry. To assess the involvement of RevS in virulence regulation, the revS gene was inactivated by Targetron insertion. When isogenic wild-type, revS and complemented revS strains were tested in a mouse myonecrosis model, the revS mutant was found to be attenuated in virulence, which was similar to the attenuation observed previously with the revR mutant. However, transcriptional analysis of selected RevR-regulated genes in the revS mutant revealed a different pattern of expression to a revR mutant, suggesting that the RevSR system is more complex than originally thought. Taken together, the results have led to the identification and characterization of the two essential parts of a new regulatory network that is involved in the regulation of virulence in C. perfringens. Copyright © 2016 Elsevier GmbH. All rights reserved.

  3. Millimeter-wave integrated-horn antennas. I - Theory. II - Experiment

    NASA Technical Reports Server (NTRS)

    Eleftheriades, George V.; Ali-Ahmad, Walid Y.; Katehi, Linda P. B.; Rebeiz, Gabriel M.

    1991-01-01

    Full-wave analysis is employed to determine the far-field pattern and input impedance of a dipole-fed horn antenna in a ground plane, and the theoretical results are compared with mm-wave and microwave data. The theoretical work exploits the Green's function corresponding to the horn structure and the method of moments. It is determined that the horn should have 70 sections/wavelength and 50 secondary modes for optimized accuracy, and certain dipole positions can reduce the resonance to zero. The experimentally derived impedance and radiation patterns agree with the constraints developed theoretically. The 70-degree flare-angle horn with selected dipole positions and horn apertures yields good radiation patterns, cross-polarization levels, and resonant dipole impedances. The conclusions are of interest to the development of the horn antennas etched in Si/GaAs for applications to zero-visibility tracking, radio astronomy, plasma diagnostics, and remote sensing.

  4. Millimeter-wave integrated-horn antennas. I - Theory. II - Experiment

    NASA Technical Reports Server (NTRS)

    Eleftheriades, George V.; Ali-Ahmad, Walid Y.; Katehi, Linda P. B.; Rebeiz, Gabriel M.

    1991-01-01

    Full-wave analysis is employed to determine the far-field pattern and input impedance of a dipole-fed horn antenna in a ground plane, and the theoretical results are compared with mm-wave and microwave data. The theoretical work exploits the Green's function corresponding to the horn structure and the method of moments. It is determined that the horn should have 70 sections/wavelength and 50 secondary modes for optimized accuracy, and certain dipole positions can reduce the resonance to zero. The experimentally derived impedance and radiation patterns agree with the constraints developed theoretically. The 70-degree flare-angle horn with selected dipole positions and horn apertures yields good radiation patterns, cross-polarization levels, and resonant dipole impedances. The conclusions are of interest to the development of the horn antennas etched in Si/GaAs for applications to zero-visibility tracking, radio astronomy, plasma diagnostics, and remote sensing.

  5. Offshore drilling and production structure

    SciTech Connect

    Crockett, R.K.; Palmer, H.E.; Stenning, D.G.

    1982-02-09

    The invention relates to an off-shore marine structure that provides an elevated support for a drilling and/or production platform. A structure comprised of three interlocking components is provided, the first component being a large foundation base installed on the sea bed; the second being a conical shaped support component which is engagable with the foundation base and which, releasably carries the third platform supporting component. In the preferred form, the platform supporting component comprises a centrally-disposed vertical column, means being provided to facilitate engagement of the column with the platform and the second component and to subsequently elevate the platform to an operating height above sea level.

  6. Bundled pipe speeds offshore laying

    SciTech Connect

    Brockbank, J. )

    1990-05-07

    Technology which allows pipelines to be installed in bundles is expediting pipelay operations in the North Sea. This paper reports how the piggyback system was recently used on 60 km of North Sea gas pipelines for three major projects. For 7 years the practice of installing two or more pipelines in one operation has become an established practice for North Sea offshore oil and gas projects. The technique, commonly referred to as a piggyback operation, reduces installation costs, improves operation reliability, and cuts maintenance time.

  7. Wave slamming on offshore structures

    NASA Astrophysics Data System (ADS)

    Miller, B. L.

    1980-03-01

    Experimental and theoretical work on the slamming of circular cylinders is surveyed. Data are included from controlled drop tests. The influence of inclined impact and beam dynamics on the resulting stresses is calculated for a wide range of wave conditions. The statistical distributions of the estimated stresses are analyzed to provide data for the calculation of slamming loads on fixed offshore structures using simple formulas in which the slamming coefficients incorporate both the member dynamics and the sea wave statistics. Slamming coefficients and associated stress calculation methods are presented for extreme values and fatigue damage. These may also be used for slamming during jacket launching. A film of wave slam was also produced.

  8. Seismic assessment for offshore pipelines

    SciTech Connect

    Bruschi, R.; Gudmestad, O.T.; Blaker, F.; Nadim, F.

    1995-12-31

    An international consensus on seismic design criteria for onshore pipelines has been established during the last thirty years. The need to assess seismic design for offshore pipelines has not been similarly recognized. In this paper, the geotechnical hazard for a pipeline routed across steep slopes and irregular terrains affected by earthquakes, is discussed. The integrity of both natural and artificial load bearing supports is assessed.d The response of the pipeline to direct excitation from soil or through discontinuous, sparsely distributed natural or artificial supports, is commented.

  9. Studies on thermo-elastic heating of horns used in ultrasonic plastic welding.

    PubMed

    Roopa Rani, M; Prakasan, K; Rudramoorthy, R

    2015-01-01

    Ultrasonic welding horn is half wavelength section or tool used to focus the ultrasonic vibrations to the components being welded. The horn is designed in such a way that it maximizes the amplitude of the sound wave passing through it. The ends of the horn represent the displacement anti-nodes and the center the 'node' of the wave. As the horns perform 20,000 cycles of expansion and contraction per second, they are highly stressed at the nodes and are heated owing to thermo-elastic effects. Considerable temperature rise may be observed in the horn, at the nodal region when working at high amplitudes indicating high stress levels leading to failure of horns due to cyclic loading. The limits for amplitude must therefore be evaluated for the safe working of the horn. Horns made of different materials have different thermo-elastic behaviors and hence different temperatures at the nodes and antinodes. This temperature field can be used as a control mechanism for setting the amplitude/weld parameters. Safe stress levels can be predicted using modal and harmonic analyses followed by a stress analysis to study the effect of cyclic loads. These are achieved using 'Ansys'. The maximum amplitude level obtained from the stress analysis is used as input for 'Comsol' to predict the temperature field. The actual temperature developed in the horn during operation is measured using infrared camera and compared with the simulated temperature. From experiments, it is observed that horn made of titanium had the lowest temperature rise at the critical region and can be expected to operate at amplitudes up to 77 μm without suffering failure due to cyclic loading. The method of predicting thermo-elastic stresses and temperature may be adopted by the industry for operating the horn within the safe stress limits thereby extending the life of the horn.

  10. Improving road safety and residential quality of life : evaluating the automated wayside horn system.

    PubMed

    Lucke, Roy E; Raub, Richard A; Thunder, Thomas E

    2004-01-01

    The automated wayside horn system is designed to replace the train horn as a means of alerting motorists to danger and thus enhancing safety at highway-rail grade crossings. Furthermore, the wayside horn directionality is such that the warning sound is broadcast over a smaller sector than the train horn, thereby reducing residential noise. This article examines the results of an evaluation comparing train horns with wayside horns in the village of Mundelein, Illinois, USA. The study derived from previous work in Gering, Nebraska, and Ames, Iowa.During the 3 months covering the 'before' (train horn) period and through to 'after' (wayside horn), more than 19 500 crossing gate closures were recorded on videotape at three crossings. Analysis showed motorist violation of level-crossing laws decreased 68%, from an average rate of 3.53 per 100 gate closings when train horns were in use to 1.12 per 100 with the wayside horn. The decrease was statistically significant. Of equal importance was the decrease in residential noise. Sound measurements taken in a sample of residential yards showed a decrease in sound levels by more than 10 decibels (dB) at most locations. When plotted as sound contours, decreases in the area of coverage ranged from 85% at the 90dB level to 65% at the 70dB level.However, there are two issues with the use of wayside horns that need to be resolved. First, and most important, is that the wayside horn starts sounding when the warning lights begin to flash. This startles motorists, and some stop on the rail tracks. A second issue is the frequent unwarranted activation of the system, which encourages people to ignore the gate.

  11. Acute abdomen in a case with noncommunicating rudimentary horn and unicornuate uterus.

    PubMed

    Atmaca, Rusen; Germen, Aysegul Tezcan; Burak, Feza; Kafkasli, Ayse

    2005-01-01

    Unicornuate uterus with a rudimentary horn is the rarest congenital anatomic anomaly of the female genital system, causing many obstetrical and gynecologic complications. The frequency of this pathology is approximately 1/100 000. A rudimentary horn usually develops following insufficient development of mullerian ducts. These patients present with dysmenorrhea, dyspareunia, and chronic pelvic pain because of endometriosis and rarely with acute abdominal symptoms following distention and torsion of the noncommunicating rudimentary horn. The case of a patient referred for acute abdomen after distention of a noncommunicating rudimentary horn is presented herein.

  12. Acute Abdomen in a Case With Noncommunicating Rudimentary Horn and Unicornuate Uterus

    PubMed Central

    Germen, Aysegul Tezcan; Burak, Feza; Kafkasli, Ayse

    2005-01-01

    Unicornuate uterus with a rudimentary horn is the rarest congenital anatomic anomaly of the female genital system, causing many obstetrical and gynecologic complications. The frequency of this pathology is approximately 1/100 000. A rudimentary horn usually develops following insufficient development of mullerian ducts. These patients present with dysmenorrhea, dyspareunia, and chronic pelvic pain because of endometriosis and rarely with acute abdominal symptoms following distention and torsion of the noncommunicating rudimentary horn. The case of a patient referred for acute abdomen after distention of a noncommunicating rudimentary horn is presented herein. PMID:15984720

  13. Computation of reflected and transmitted horn radiation patterns for a dichroic plate

    NASA Technical Reports Server (NTRS)

    Chen, J. C.

    1994-01-01

    A previous dichroic plate analysis has assumed that an ideal uniform plane wave illuminates the dichroic plate at a single angle of incidence. In fact, a horn radiates energy at the dichroic plate and illuminates it at many different angles. To model the horn and dichroic plate system, the horn pattern is represented as a group of plane waves traveling in different directions. The details of this analysis are presented this article. The calculated and measured reflected radiation patterns show good agreement. The noise temperature predicted from the horn pattern model is shown to be more accurate than that from a simple plane-wave model.

  14. Domestic Options to Offshore Oil and Gas.

    ERIC Educational Resources Information Center

    Kash, Don E.

    1983-01-01

    The continuing controversey over offshore oil/gas has given impetus to searching for domestic energy alternatives. The need for and types of several alternative sources are discussed. Indicates that the United States needs to pursue both offshore and other domestic liquid-fuel sources if it is to avoid becoming increasingly dependent on imports.…

  15. U.S. Offshore Wind Port Readiness

    SciTech Connect

    C. Elkinton, A. Blatiak, H. Ameen

    2013-10-13

    This study will aid decision-makers in making informed decisions regarding the choice of ports for specific offshore projects, and the types of investments that would be required to make individual port facilities suitable to serve offshore wind manufacturing, installation and/or operations.

  16. Domestic Options to Offshore Oil and Gas.

    ERIC Educational Resources Information Center

    Kash, Don E.

    1983-01-01

    The continuing controversey over offshore oil/gas has given impetus to searching for domestic energy alternatives. The need for and types of several alternative sources are discussed. Indicates that the United States needs to pursue both offshore and other domestic liquid-fuel sources if it is to avoid becoming increasingly dependent on imports.…

  17. 31 CFR 547.406 - Offshore transactions.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 31 Money and Finance: Treasury 3 2010-07-01 2010-07-01 false Offshore transactions. 547.406 Section 547.406 Money and Finance: Treasury Regulations Relating to Money and Finance (Continued) OFFICE... REGULATIONS Interpretations § 547.406 Offshore transactions. The prohibitions in § 547.201 on transactions or...

  18. Targeted cleavage of HIV RRE RNA by Rev-coupled transition metal chelates.

    PubMed

    Joyner, Jeff C; Cowan, J A

    2011-06-29

    A series of compounds that target reactive metal chelates to the HIV-1 Rev response element (RRE) mRNA have been synthesized. Dissociation constants and chemical reactivity toward HIV RRE RNA have been determined and evaluated in terms of reduction potential, coordination unsaturation, and overall charge associated with the metal-chelate-Rev complex. Ethylenediaminetetraacetic acid (EDTA), nitrilotriacetic acid (NTA), diethylenetriaminepentaacetic acid (DTPA), and 1,4,7,10-tetraazacyclododecane-1,4,7,10-tetraacetic acid (DOTA) were linked to a lysine side chain of a Rev-derived peptide by either EDC/NHS or isothiocyanate coupling. The resulting chelate-Rev (EDTA-Rev, DTPA-Rev, NTA-Rev, and DOTA-Rev) conjugates were used to form coordination complexes with Fe(2+), Co(2+), Ni(2+), and Cu(2+) such that the arginine-rich Rev peptide could mediate localization of the metal chelates to the Rev peptide's high-affinity mRNA binding partner, RRE stem loop IIB. Metal complexes of the extended peptides GGH-Rev and KGHK-Rev, which also contain N-terminal peptidic chelators (ATCUN motifs), were studied for comparison. A fluorescence titration assay revealed high-affinity RRE RNA binding by all 22 metal-chelate-Rev species, with K(D) values ranging from ~0.2 to 16 nM, indicating little to no loss of RNA affinity due to the coupling of the metal chelates to the Rev peptide. Dissociation constants for binding at a previously unobserved low-affinity site are also reported. Rates of RNA modification by each metal-chelate-Rev species were determined and varied from ~0.28 to 4.9 nM/min but were optimal for Cu(2+)-NTA-Rev. Metal-chelate reduction potentials were determined and varied from -228 to +1111 mV vs NHE under similar solution conditions, allowing direct comparison of reactivity with redox thermodynamics. Optimal activity was observed when the reduction potential for the metal center was poised between those of the two principal co-reagents for metal-promoted formation of

  19. Structural features offshore northern Taiwan

    NASA Astrophysics Data System (ADS)

    Yicheng Yang, Eason; Liu, Char-Shine; Chang, Jih-Hsin; Chiu, Chien-Hsuan

    2016-04-01

    The area offshore northern Taiwan is the place where East China Sea Shelf extends into the Southern Okinawa Trough, and where pre-Pleistocene arc-continental collision had occurred. Comparison between fault distribution in the area with previously published results suggests that the fault distribution and regional structural framework are still controversial. Using marine multichannel seismic reflection data collected in 3 marine geophysical survey cruises, we remapped the fault distribution in the northern offshore area of Taiwan. By analyzing all the seismic profiles using the KINGDOM suite (a seismic interpretation software), a new fault distribution map is presented, and a subsurface unconformity PRSB (Pliocene reflection sequence boundary) is identified. Six major NE-SW trending high-angle normal faults cut the PRSB can be traced to the fault systems on land northernmost Taiwan. These normal faults are located between the Southern Okinawa Trough and the East China Sea continental shelf basin, and have been suggested to be reactivated from pre-existing reverse faults. The offsets of fault ramps in PRSB increase toward southeast. The isopach map of the study area compiled shows that sediment strata overlying PRSB thin toward northwest.

  20. Mouse Rev1 protein interacts with multiple DNA polymerases involved in translesion DNA synthesis

    PubMed Central

    Guo, Caixia; Fischhaber, Paula L.; Luk-Paszyc, Margaret J.; Masuda, Yuji; Zhou, Jing; Kamiya, Kenji; Kisker, Caroline; Friedberg, Errol C.

    2003-01-01

    Polκ and Rev1 are members of the Y family of DNA polymerases involved in tolerance to DNA damage by replicative bypass [translesion DNA synthesis (TLS)]. We demonstrate that mouse Rev1 protein physically associates with Polκ. We show too that Rev1 interacts independently with Rev7 (a subunit of a TLS polymerase, Polζ) and with two other Y-family polymerases, Polι and Polη. Mouse Polκ, Rev7, Polι and Polη each bind to the same ∼100 amino acid C-terminal region of Rev1. Furthermore, Rev7 competes directly with Polκ for binding to the Rev1 C-terminus. Notwith standing the physical interaction between Rev1 and Polκ, the DNA polymerase activity of each measured by primer extension in vitro is unaffected by the complex, either when extending normal primer-termini, when bypassing a single thymine glycol lesion, or when extending certain mismatched primer termini. Our observations suggest that Rev1 plays a role(s) in mediating protein–protein interactions among DNA polymerases required for TLS. The precise function(s) of these interactions during TLS remains to be determined. PMID:14657033

  1. Human REV3 DNA Polymerase Zeta Localizes to Mitochondria and Protects the Mitochondrial Genome.

    PubMed

    Singh, Bhupendra; Li, Xiurong; Owens, Kjerstin M; Vanniarajan, Ayyasamy; Liang, Ping; Singh, Keshav K

    2015-01-01

    To date, mitochondrial DNA polymerase γ (POLG) is the only polymerase known to be present in mammalian mitochondria. A dogma in the mitochondria field is that there is no other polymerase present in the mitochondria of mammalian cells. Here we demonstrate localization of REV3 DNA polymerase in the mammalian mitochondria. We demonstrate localization of REV3 in the mitochondria of mammalian tissue as well as cell lines. REV3 associates with POLG and mitochondrial DNA and protects the mitochondrial genome from DNA damage. Inactivation of Rev3 leads to reduced mitochondrial membrane potential, reduced OXPHOS activity, and increased glucose consumption. Conversely, inhibition of the OXPHOS increases expression of Rev3. Rev3 expression is increased in human primary breast tumors and breast cancer cell lines. Inactivation of Rev3 decreases cell migration and invasion, and localization of Rev3 in mitochondria increases survival and the invasive potential of cancer cells. Taken together, we demonstrate that REV3 functions in mammalian mitochondria and that mitochondrial REV3 is associated with the tumorigenic potential of cells.

  2. The Nuclear Receptor Rev-erbα Controls Circadian Thermogenic Plasticity

    PubMed Central

    Gerhart-Hines, Zachary; Everett, Logan J.; Loro, Emanuele; Briggs, Erika R.; Bugge, Anne; Hou, Catherine; Ferrara, Christine; Seale, Patrick; Pryma, Daniel A.; Khurana, Tejvir S.; Lazar, Mitchell A.

    2013-01-01

    Circadian oscillation of body temperature is a basic, evolutionary-conserved feature of mammalian biology1. Additionally, homeostatic pathways allow organisms to protect their core temperatures in response to cold exposure2. However, the mechanism responsible for coordinating daily body temperature rhythm and adaptability to environmental challenges is unknown. Here we show that the nuclear receptor Rev-erbα, a powerful transcriptional repressor, links circadian and thermogenic networks through the regulation of brown adipose tissue (BAT) function. Mice exposed to cold fare dramatically better at 5 AM (Zeitgeber time 22) when Rev-erbα is barely expressed than at 5 PM (ZT10) when Rev-erbα is abundant. Deletion of Rev-erbα markedly improves cold tolerance at 5 PM, indicating that overcoming Rev-erbα-dependent repression is a fundamental feature of the thermogenic response to cold. Physiological induction of uncoupling protein 1 (UCP1) by cold temperatures is preceded by rapid down-regulation of Rev-erbα in BAT. Rev-erbα represses UCP1 in a brown adipose cell-autonomous manner and BAT UCP1 levels are high in Rev-erbα-null mice even at thermoneutrality. Genetic loss of Rev-erbα also abolishes normal rhythms of body temperature and BAT activity. Thus, Rev-erbα acts as a thermogenic focal point required for establishing and maintaining body temperature rhythm in a manner that is adaptable to environmental demands. PMID:24162845

  3. RNA-guided assembly of Rev-RRE nuclear export complexes.

    PubMed

    Bai, Yun; Tambe, Akshay; Zhou, Kaihong; Doudna, Jennifer A

    2014-08-27

    HIV replication requires nuclear export of unspliced and singly spliced viral transcripts. Although a unique RNA structure has been proposed for the Rev-response element (RRE) responsible for viral mRNA export, how it recruits multiple HIV Rev proteins to form an export complex has been unclear. We show here that initial binding of Rev to the RRE triggers RNA tertiary structural changes, enabling further Rev binding and the rapid formation of a viral export complex. Analysis of the Rev-RRE assembly pathway using SHAPE-Seq and small-angle X-ray scattering (SAXS) reveals two major steps of Rev-RRE complex formation, beginning with rapid Rev binding to a pre-organized region presenting multiple Rev binding sites. This step induces long-range remodeling of the RNA to expose a cryptic Rev binding site, enabling rapid assembly of additional Rev proteins into the RNA export complex. This kinetic pathway may help maintain the balance between viral replication and maturation.DOI: http://dx.doi.org/10.7554/eLife.03656.001.

  4. Underbalanced drilling benefits now available offshore

    SciTech Connect

    Vozniak, J.P.; Cuthbertson, B.; Nessa, D.O.

    1997-05-01

    Offshore underbalanced drilling (UBD) is a reality. Applications in older, partially depleted fields and new fields are being considered. However, low productivity reservoirs and fields with sub normal pressures causing drilling problems are currently the main targets for offshore UBD. With proper planning and the correct technique, both jointed pipe and coiled tubing UBD drilling operations have been carried out offshore with success. The main concerns for offshore UBD have been altered drilling practices and surface production system operation. These issues have been examined and equipment has been designed and tested to address them. Environmental, safety and health issues are paramount and have been studied carefully. Detailed well planning, engineering, and flow modeling have proven critical for successful offshore UBD operations. Examples are given from oil and gas fields.

  5. Offshore Wind Market and Economic Analysis

    SciTech Connect

    Hamilton, Bruce Duncan

    2014-08-27

    This report is the third annual assessment of the U.S. offshore wind market. It includes the following major sections: Section 1: key data on developments in the offshore wind technology sector and the global development of offshore wind projects, with a particular focus on progress in the United States; Section 2: analysis of policy developments at the federal and state levels that have been effective in advancing offshore wind deployment in the United States; Section 3: analysis of actual and projected economic impact, including regional development and job creation; Section 4: analysis of developments in relevant sectors of the economy with the potential to affect offshore wind deployment in the United States

  6. REV3L modulates cisplatin sensitivity of non-small cell lung cancer H1299 cells.

    PubMed

    Wang, Wenjie; Sheng, Wenjiong; Yu, Chenxiao; Cao, Jianping; Zhou, Jundong; Wu, Jinchang; Zhang, Huojun; Zhang, Shuyu

    2015-09-01

    Lung cancer remains the leading cause of cancer-related mortality worldwide and non-small cell lung cancer (NSCLC) accounts for approximately 80-85% of all cases of lung cancer. Cisplatin plays a significant role in the management of human lung cancer. Translesion DNA synthesis (TLS) is involved in DNA damage repair. DNA polymerase ζ (Pol ζ) is able to mediate the DNA replication bypass of DNA damage, which is suggested to be involved in chemoresistance. REV3L is the catalytic subunit of Pol ζ. Due to its critical role in translesion DNA synthesis, whether REV3L modulates cisplatin response in NSCLC cells remains unknown. In this study, REV3L overexpression and silencing H1299 cell lines were established. The reports showed that cisplatin induced the expression of REV3L by recruiting Sp1 to its promoter. Similar results were obtained when the ability of the cells to express luciferase from a platinated plasmid was measured. Co-transfection of the reporter with the REV3L overexpression vector or REV3L plus REV7L significantly enhanced the reporter activity. Nuclear condensation and fragmentation of shRNA-REV3L H1299 cells were more pronounced than shRNA-NC H1299 cells after cisplatin exposure, indicating that REV3L overexpression abolished cisplatin-induced DNA damage. Moreover, a forced expression of REV3L conferred the resistance of H1299 cells to cisplatin, whereas the knockdown of REV3L sensitized cisplatin efficacy in H1299 cells. Taken together, we demonstrated that inhibition of REV3L sensitized lung cancer H1299 cells to cisplatin treatment. Thus, REV3L may be a novel target for the chemotherapy of NSCLC.

  7. An ultrasonic horn atomizer with closed loop driving circuit

    NASA Astrophysics Data System (ADS)

    Chou, Yuan-Fang; Chen, Kai-Jhong; Hsu, Jui-Mei; Chou, Pei-En

    2016-04-01

    A novel ultrasonic horn atomizer is developed for the purpose of obtaining small size droplets at a large flow rate. The ultrasonic horn has a non-monotonically decreasing cross sectional area to provide a large atomizing surface. Consisting of two horns and one actuator section, the 301 kHz atomizer nozzle is made of {100} silicon wafer with its axis aligned in the <100> direction to minimize the length. Two PZT plates are adhered to each side of the actuator section to provide driving power. This device atomizes the liquid film on its nozzle tip to generate droplets. It is capable of atomizing more than 350 μl/min water into droplet. The mean diameter of droplet is 9.61 μm and the size distribution is quite narrow. The atomizing mechanism is based on the capillary wave on liquid surface. Once the wave amplitude exceeds the critical value, the motion of surface liquid becomes unstable and releases droplets. Therefore, driving at resonant frequency is the most effective way for atomizing. Dimension deviation combined with different kind of liquid to be atomized causes resonant frequencies of nozzles changed from time to time. Due to the high Q nature of nozzles, atomizing performance will drop drastically once the driving frequency is different from its resonant frequency by very little amount. Therefore, a feedback circuit is designed to tracking resonant frequency automatically instead of adjusting driving frequency manually. Comparing the atomizing performance between the open loop system and the closed loop system, significant improvement is obtained.

  8. Emerging landscape degradation trends in the East African Horn

    NASA Astrophysics Data System (ADS)

    Pricope, N. G.; Michaelsen, J.; Husak, G. J.; Funk, C. C.; Lopez-Carr, D.

    2012-12-01

    Increasing climate variability along with declining trends in rainfall represent major risk factors affecting food security in many regions of the world. We identify Africa-wide regions where significant rainfall decreases from 1979-2011 are coupled with significant human population density increases. The rangelands of the East African Horn remain one of the world's most food insecure regions with significantly increasing human populations predominantly dependent on pastoralist and agro-pastoralist livelihoods. Widespread vegetation degradation is occurring, adversely impacting fragile ecosystems and human livelihoods. Using MODIS land cover and normalized difference vegetation index (NDVI) data collected since 2000, we observe significant changes in vegetation patterns and productivity over the last decade across the East African Horn and demonstrate that these two products can be used concurrently at large spatial scales to monitor vegetation dynamics at decadal time scales. Results demonstrate that a near doubling of the population in pastoral regions is linked with hotspots of degradation in vegetation condition. The most significant land cover change and browning trends are observed in areas experiencing drying precipitation trends in addition to increasing population pressures. These findings have serious implications for current and future regional food security monitoring and forecasting and for mitigation and adaptation strategies in a region where population is expected to continue increasing against a backdrop of drying climate trends.Fig.1(a)Change in standardized precipitation index in Africa between 1979-2010 (b)Change in population density at continental scale using the GRUMPv1 1990 and 2000 and AfriPop 2010 population density datasets Fig.2 Land cover change trajectories based on 2001-2009 MOD12Q1 Land Cover product for the East African Horn overlaid over aggregated FEWS Net Livelihoods Zones.

  9. Surveying woodland hawks with broadcasts of great horned owl vocalization

    USGS Publications Warehouse

    Mosher, James A.; Fuller, Mark R.

    1996-01-01

    Pre-recorded vocalizations of great horned owls (Bubo virginianus) broadcast into predominantly wooded habitat along roadside survey routes resulted in as many detections of resident red-shouldered hawks (Buteo lineatus) and Cooper's hawks (Accipiter cooperii) as broadcasts of each conspecific calls. Survey results for 3 species, expressed as average number of contacts/route, were directly related to the number of resident pairs located during systematic searches conducted on foot across the study area. Regression models based on road-transect counts were significant for predicting abundance of red-shouldered hawks, broad-winged hawks (Buteo platypterus), and Cooper's hawks from our study areas.

  10. A strange horn between Paolo Mantegazza and Charles Darwin.

    PubMed

    Garbarino, Carla; Mazzarello, Paolo

    2013-09-01

    During the preparation of an exhibition in Pavia dedicated to the centennial anniversary of the death of the Italian Pathologist Paolo Mantegazza, a strange cheratinic horn was found at the Museum for the History of the University of Pavia labelled as 'spur of a cock transplanted into an ear of a cow.' After some historical investigation, we found this strange object was at the centre of a scientific correspondence between Mantegazza and Charles Darwin, who made reference to it in his book The Variation of Animals and Plants under Domestication.

  11. Entanglement Distillation from Greenberger-Horne-Zeilinger Shares

    NASA Astrophysics Data System (ADS)

    Vrana, Péter; Christandl, Matthias

    2017-03-01

    We study the problem of converting a product of Greenberger-Horne-Zeilinger (GHZ) states shared by subsets of several parties in an arbitrary way into GHZ states shared by every party. Such a state can be described by a hypergraph on the parties as vertices and with each hyperedge corresponding to a GHZ state shared among the parties incident with it. Our result is that if SLOCC transformations are allowed, then the best asymptotic rate is the minimum of bipartite log-ranks of the initial state, which in turn equals the minimum cut of the hypergraph. This generalizes a result by Strassen on the asymptotic subrank of the matrix multiplication tensor.

  12. Entanglement Distillation from Greenberger-Horne-Zeilinger Shares

    NASA Astrophysics Data System (ADS)

    Vrana, Péter; Christandl, Matthias

    2017-06-01

    We study the problem of converting a product of Greenberger-Horne-Zeilinger (GHZ) states shared by subsets of several parties in an arbitrary way into GHZ states shared by every party. Such a state can be described by a hypergraph on the parties as vertices and with each hyperedge corresponding to a GHZ state shared among the parties incident with it. Our result is that if SLOCC transformations are allowed, then the best asymptotic rate is the minimum of bipartite log-ranks of the initial state, which in turn equals the minimum cut of the hypergraph. This generalizes a result by Strassen on the asymptotic subrank of the matrix multiplication tensor.

  13. Asymmetric multipartite Greenberger-Horne-Zeilinger states and Bell inequalities

    SciTech Connect

    Gosal, Darwin; Oh, C.H.; Kaszlikowski, Dagomir; Kwek, L.C.; Zukowski, M.

    2004-10-01

    We study the multiparticle generalized GHZ states. It has been shown that for an odd number of qubits and for a specific range of parameters, they do not violate any Bell inequality for correlation functions. We show here both analytically and numerically that, nevertheless, such states violate local realism, once a more detailed analysis of the correlations is made than the one allowed by correlation functions. The results imply that multiparticle Clauser-Horne-type inequalities involving probabilities are stronger tools for analyzing violations of local realism in multiparticle systems than inequalities involving the correlation functions.

  14. Proteasomal regulation of the mutagenic translesion DNA polymerase, Saccharomyces cerevisiae Rev1.

    PubMed

    Wiltrout, Mary Ellen; Walker, Graham C

    2011-02-07

    Translesion DNA synthesis (TLS) functions as a tolerance mechanism for DNA damage at a potentially mutagenic cost. Three TLS polymerases (Pols) function to bypass DNA damage in Saccharomyces cerevisiae: Rev1, Pol ζ, a heterodimer of the Rev3 and Rev7 proteins, and Pol η (Rad30). Our lab has shown that S. cerevisiae Rev1 protein levels are under striking cell cycle regulation, being ∼50-fold higher during G2/M than during G1 and much of S phase (Waters and Walker, 2006). REV1 transcript levels only vary ∼3-fold in a similar cell cycle pattern, suggesting a posttranscriptional mechanism controls protein levels. Here, we show that the S. cerevisiae Rev1 protein is unstable during both the G1 and the G2/M phases of the cell cycle, however, the protein's half-life is shorter in G1 arrested cells than in G2/M arrested cells, indicating that the rate of proteolysis strongly contributes to Rev1's cell cycle regulation. In the presence of the proteasome inhibitor, MG132, the steady-state levels and half-life of Rev1 increase during G1 and G2/M. Through the use of a viable proteasome mutant, we confirm that the levels of Rev1 protein are dependent on proteasome-mediated degradation. The accumulation of higher migrating forms of Rev1 under certain conditions shows that the degradation of Rev1 is possibly directed through the addition of a polyubiquitination signal or another modification. These results support a model that proteasomal degradation acts as a regulatory system of mutagenic TLS mediated by Rev1.

  15. New Rev-export inhibitor from Alpinia galanga and structure-activity relationship.

    PubMed

    Tamura, Satoru; Shiomi, Atsushi; Kaneko, Masafumi; Ye, Ying; Yoshida, Minoru; Yoshikawa, Masayuki; Kimura, Tominori; Kobayashi, Motomasa; Murakami, Nobutoshi

    2009-05-01

    Bioassay-guided separation by use of the fission yeast expressing NES of Rev, an HIV-1 viral regulatory protein, disclosed 1'-acetoxychavicol acetate (ACA, 1) as a new inhibitor for nuclear export of Rev from the roots of Alpinia galanga. Both analysis for mechanism of action with biotinylated probe (2) and several synthesized analogs established crucial portions in 1 for Rev-export inhibitory activity.

  16. Offshore Wind Energy Systems Engineering Curriculum Development

    SciTech Connect

    McGowan, Jon G.; Manwell, James F.; Lackner, Matthew A.

    2012-12-31

    Utility-scale electricity produced from offshore wind farms has the potential to contribute significantly to the energy production of the United States. In order for the U.S. to rapidly develop these abundant resources, knowledgeable scientists and engineers with sound understanding of offshore wind energy systems are critical. This report summarizes the development of an upper-level engineering course in "Offshore Wind Energy Systems Engineering." This course is designed to provide students with a comprehensive knowledge of both the technical challenges of offshore wind energy and the practical regulatory, permitting, and planning aspects of developing offshore wind farms in the U.S. This course was offered on a pilot basis in 2011 at the University of Massachusetts and the National Renewable Energy Laboratory (NREL), TU Delft, and GL Garrad Hassan have reviewed its content. As summarized in this report, the course consists of 17 separate topic areas emphasizing appropriate engineering fundamentals as well as development, planning, and regulatory issues. In addition to the course summary, the report gives the details of a public Internet site where references and related course material can be obtained. This course will fill a pressing need for the education and training of the U.S. workforce in this critically important area. Fundamentally, this course will be unique due to two attributes: an emphasis on the engineering and technical aspects of offshore wind energy systems, and a focus on offshore wind energy issues specific to the United States.

  17. NREL Offshore Balance-of-System Model

    SciTech Connect

    Maness, Michael; Maples, Benjamin; Smith, Aaron

    2017-01-01

    The U.S. Department of Energy (DOE) has investigated the potential for 20% of nationwide electricity demand to be generated from wind by 2030 and, more recently, 35% by 2050. Achieving this level of wind power generation may require the development and deployment of offshore wind technologies. DOE (2008) has indicated that reaching these 2030 and 2050 scenarios could result in approximately 10% and 20%, respectively, of wind energy generation to come from offshore resources. By the end of 2013, 6.5 gigawatts of offshore wind were installed globally. The first U.S. project, the Block Island Wind Farm off the coast of Rhode Island, has recently begun operations. One of the major reasons that offshore wind development in the United States is lagging behind global trends is the high capital expenditures required. An understanding of the costs and associated drivers of building a commercial-scale offshore wind plant in the United States will inform future research and help U.S. investors feel more confident in offshore wind development. In an effort to explain these costs, the National Renewable Energy Laboratory has developed the Offshore Balance-of-System model.

  18. Offshore Fish Community: Ecological Interactions | Science ...

    EPA Pesticide Factsheets

    The offshore (>80 m) fish community of Lake Superior is made up of predominately native species. The most prominent species are deepwater sculpin, kiyi, cisco, siscowet lake trout, burbot, and the exotic sea lamprey. Bloater and shortjaw cisco are also found in the offshore zone. Bloater is abundant in the offshore zone but appears restricted to depths shallower than 150 m (Selgeby and Hoff 1996; Stockwell et al. 2010), although it occuppied greater depths several decades ago (Dryer 1966; Peck 1977). Shortjaw is relatively rare in the offshore zone (Hoff and Todd 2004; Gorman and Hoff 2009; Gorman and Todd 2007). Lake whitefish is also known to frequent bathymetric depths >100 m (Yule et al. 2008b). In this chapter, we develop a conceptual model of the offshore food web based on data collected during 2001-2005 and on inferences from species interactions known for the nearshore fish community. We then develop a framework for examination of energy and nutrient movements within the pelagic and benthic habitats of the offshore zone and across the offshore and nearshore zones. To document research results.

  19. Installation of an offshore structure

    SciTech Connect

    Will, S.A.; Young, C.E.

    1981-04-07

    In the installation of a jacket or substructure component of an offshore platform on the sea floor over an underwater fixture containing one or more wellheads, a jacket is first ballasted to rest in a vertical orientation on the sea floor. Power winches are mounted atop the jacket above the water line and anchored mooring lines are connected to the winches via fairlead sheaves which define points of attachment for the mooring lines to the jacket. The jacket is deballasted to float with a near sea bottom clearance and is maneuvered horizontally with the power winches toward the underwater fixture. Docking guides carried by the jacket engage vertical guideposts driven drilled into the sea floor at preselected locations relative to the underwater fixture to align the jacket with the underwater fixture. The jacket is then lowered into the desired on-bottom position during controlled ballasting procedures. Finally, piles are driven through hollow jacket columns to anchor the jacket to the sea bottom.

  20. Mifepristone-induced abortion in one horn and a growing fetus in another horn in a patient with a twin pregnancy in a bicornuate uterus.

    PubMed

    Chao, Angel; Chao, An-Shine; Wang, Shih-Tíen; Wang, Tzu-Hao

    2006-12-01

    To report a case of mifepristone-induced abortion in only one horn but a growing fetus in another horn for a twin pregnancy in a bicornuate uterus. Case report. Department of Obstetrics and Gynecology, Chang Gung Memorial Hospital, Taiwan. A 31-year-old woman who conceived spontaneously presented with 37 days' gestation. Mifepristone 600 mg orally followed by misoprostol 400 mug orally 2 days later. Termination of pregnancy. Induced abortion in only one horn but a growing fetus in another horn for a twin pregnancy in the bicornuate uterus detected by ultrasound. Surgical abortion with vacuum curettage was performed, and menstruation resumed 1 month later. As medical abortion with mifepristone and misoprostol becomes more prevalent, more studies are necessary to establish the dosage and regimens for multiple pregnancies associated with uterine anomalies.

  1. Geology of offshore central California

    SciTech Connect

    McCulloch, D.S.; Lewis, S.D.

    1988-03-01

    The offshore central California margin records Mesozoic and Cenozoic events attributable to continental and oceanic plate interactions. Terranes, some carried thousands of kilometers northward on the Kula and/or Farallon plates, were accreted to North America by late Eocene. Following accretion to North America, terranes along the margin were silvered and redistributed by strike-slip motion along the San Andreas and related faults. During Oligocene global low sea level, much of the coastal margin was subareal and erosion stripped Upper Cretaceous and Eocene strata from elevated basement blocks. These basement blocks subsequently subsided along large-displacement near-coastal faults, and shallow-water marine upper Oligocene and/or lower Miocene volcanics on the present shelf were covered by hemipelagic sediment at bathyal to mid-bathyal depths. This subsidence coincided with tectonism and significant changes in water depth elsewhere around the Pacific margin, and may have been related to a change in Pacific and North American plate relative motion that produced transtensional motion along the California margin. Transtension may have initiated both rupture and volcanism along the San Andreas fault system at about 23 Ma and wrench tectonic deformation on the shelf. During the late Miocene, growing structural highs on the shelf edge formed elongate shelf basins. A compressional component in Pacific and North America plate relative motion, which began between 5 and 3 Ma, produced strike-parallel folds and compressional faults that now dominate the structural grain of the offshore basins. Continued compressive deformation along coastal California is documented by active thrust faults, rapid coastal uplift, and earthquake focal mechanism solutions indicative of northeast-southwest compression.

  2. [Renal function after surgical treatment of stag-horned nephrolithiasis].

    PubMed

    Akulin, S M; Ianenko, E K; Demin, A I; Nikitinskaia, L P

    2010-01-01

    Renal function in patients with stag-horn nephrolithiasis was assessed after two surgical treatments--percutaneous nephrolitholapaxy (PCN L) and pyelonephrolithotomy. The function of the operated and contralateral kidneys was studied early and late after operation by parameters of blood biochemistry, Doppler investigation of renal parenchyma, dynamic nephroscintigraphy. Stabilization or improvement of blood biochemical indices was observed in 58 (70%) patients. A secretory renal function late after surgery (from 6 months to 2 years) evidenced for improvement or stabilization of renal function (according to radionuclide test) in 71 (85.5%) patients. Integral parameters of renal blood flow were physiological or improved versus preoperative ones. PCNL was made in patients with deficient secretion less than 70%, 35 (87.5%) patients were diagnosed to have stabilization or functional improvement. After open intervention functional improvement took place in 11 (25.6%) patients, stabilization of renal function occurred in 25 (58.1%) patients. Tubular secretion deterioration was seen much more frequently in patients after open intervention. PCNL is less invasive surgical intervention than pyelonephrolithotomy as this technique produces milder surgical trauma and does not result in intraoperative renal ischemia. PCNL is the most effective surgical modality in stag-horn concrements of the kidneys and significantly expands potential of low-invasive treatment of such patients.

  3. Hox cluster genomics in the horn shark, Heterodontus francisci.

    PubMed

    Kim, C B; Amemiya, C; Bailey, W; Kawasaki, K; Mezey, J; Miller, W; Minoshima, S; Shimizu, N; Wagner, G; Ruddle, F

    2000-02-15

    Reconstructing the evolutionary history of Hox cluster origins will lead to insights into the developmental and evolutionary significance of Hox gene clusters in vertebrate phylogeny and to their role in the origins of various vertebrate body plans. We have isolated two Hox clusters from the horn shark, Heterodontus francisci. These have been sequenced and compared with one another and with other chordate Hox clusters. The results show that one of the horn shark clusters (HoxM) is orthologous to the mammalian HoxA cluster and shows a structural similarity to the amphioxus cluster, whereas the other shark cluster (HoxN) is orthologous to the mammalian HoxD cluster based on cluster organization and a comparison with noncoding and Hox gene-coding sequences. The persistence of an identifiable HoxA cluster over an 800-million-year divergence time demonstrates that the Hox gene clusters are highly integrated and structured genetic entities. The data presented herein identify many noncoding sequence motifs conserved over 800 million years that may function as genetic control motifs essential to the developmental process.

  4. Hox cluster genomics in the horn shark, Heterodontus francisci

    PubMed Central

    Kim, Chang-Bae; Amemiya, Chris; Bailey, Wendy; Kawasaki, Kazuhiko; Mezey, Jason; Miller, Webb; Minoshima, Shinsei; Shimizu, Nobuyoshi; Wagner, Günter; Ruddle, Frank

    2000-01-01

    Reconstructing the evolutionary history of Hox cluster origins will lead to insights into the developmental and evolutionary significance of Hox gene clusters in vertebrate phylogeny and to their role in the origins of various vertebrate body plans. We have isolated two Hox clusters from the horn shark, Heterodontus francisci. These have been sequenced and compared with one another and with other chordate Hox clusters. The results show that one of the horn shark clusters (HoxM) is orthologous to the mammalian HoxA cluster and shows a structural similarity to the amphioxus cluster, whereas the other shark cluster (HoxN) is orthologous to the mammalian HoxD cluster based on cluster organization and a comparison with noncoding and Hox gene-coding sequences. The persistence of an identifiable HoxA cluster over an 800-million-year divergence time demonstrates that the Hox gene clusters are highly integrated and structured genetic entities. The data presented herein identify many noncoding sequence motifs conserved over 800 million years that may function as genetic control motifs essential to the developmental process. PMID:10677514

  5. Rumen perforation caused by horn injury in two cows.

    PubMed

    Braun, Ueli; Gerspach, Christian; Stettler, Manuela; Grob, Daniela; Sydler, Titus

    2016-01-20

    Post-operative complications of trocarisation and rumenotomy are the most common causes of peritonitis associated with a rumen disorder. Since horn injury leading to rumen perforation has not previously been reported in the literature, two cows with this condition are reported. Small superficial skin lesions were observed in one of the cows and the other had a perforating skin lesion in the left abdomen. Both cows had signs of hypovolaemic shock. Ultrasonography revealed hypoechoic fluid, echoic lesions and occasional fibrinous septa caudoventral to the reticulum. Caudally the fluid extended to the left flank fold and occupied about one third of the peritoneal cavity. The area of the skin perforation in the left abdomen was swollen and the muscle layers could not be differentiated using ultrasonography. Diffuse fibrino-purulent peritonitis was diagnosed in both cows, and because of a poor prognosis, they were euthanased and necropsied. Perforation of the abdominal wall and rumen with diffuse fibrino-purulent peritonitis was present. Ultrasonography is a suitable tool to characterise the inflammatory lesions between the rumen and left abdominal wall and objectify the interpretation of clinical findings. Horn injury should be included in the rule outs for cattle with left abdominal skin wounds and diffuse peritonitis.

  6. Associations among heat shock protein 70 genotype, forage system, and horn fly infestation of beef cattle

    USDA-ARS?s Scientific Manuscript database

    Horn fly infestations on beef cattle results in decreased productivity and challenges enterprise sustainability. Objective of this experiment was to determine the relationships among, cattle breed, heat shock protein 70 (Hsp70) genotype, and horn fly density. Angus (n = 20), Brahman (n = 17), and ...

  7. 454 pyrosequencing project identifying expressed genes from the horn fly, Haematobia irritans

    USDA-ARS?s Scientific Manuscript database

    We used an EST approach to initiate a study of the genome of the horn fly, Haematobia irritans and have used 454 pyrosequencing techniques to sequence 73,512, 100,603, 71,550, and 85,769 expressed genes from the egg, first instar larvae, adult male, and adult female lifestages of the horn fly. cD...

  8. 49 CFR Appendix E to Part 222 - Requirements for Wayside Horns

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... ADMINISTRATION, DEPARTMENT OF TRANSPORTATION USE OF LOCOMOTIVE HORNS AT PUBLIC HIGHWAY-RAIL GRADE CROSSINGS Pt... following minimum requirements for wayside horn use at highway-rail grade crossings: 1. Highway-rail crossing must be equipped with constant warning time device, if reasonably practical, and...

  9. 49 CFR Appendix E to Part 222 - Requirements for Wayside Horns

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... ADMINISTRATION, DEPARTMENT OF TRANSPORTATION USE OF LOCOMOTIVE HORNS AT PUBLIC HIGHWAY-RAIL GRADE CROSSINGS Pt... following minimum requirements for wayside horn use at highway-rail grade crossings: 1. Highway-rail crossing must be equipped with constant warning time device, if reasonably practical, and...

  10. 49 CFR Appendix E to Part 222 - Requirements for Wayside Horns

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... ADMINISTRATION, DEPARTMENT OF TRANSPORTATION USE OF LOCOMOTIVE HORNS AT PUBLIC HIGHWAY-RAIL GRADE CROSSINGS Pt... following minimum requirements for wayside horn use at highway-rail grade crossings: 1. Highway-rail crossing must be equipped with constant warning time device, if reasonably practical, and...

  11. 49 CFR Appendix E to Part 222 - Requirements for Wayside Horns

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... ADMINISTRATION, DEPARTMENT OF TRANSPORTATION USE OF LOCOMOTIVE HORNS AT PUBLIC HIGHWAY-RAIL GRADE CROSSINGS Pt... following minimum requirements for wayside horn use at highway-rail grade crossings: 1. Highway-rail crossing must be equipped with constant warning time device, if reasonably practical, and...

  12. The Many Faces of Compliance: The Supreme Court's Decision in "Horne v. Flores"

    ERIC Educational Resources Information Center

    Thro, William E.

    2009-01-01

    At first blush, the Supreme Court's recent decision in "Horne v. Flores" (2009) appears to be about the proper standard for determining when to modify a previous judgment, a topic that would interest only civil procedure geeks. Yet, on closer examination, "Horne" is about giving local and state officials discretion to solve education problems and,…

  13. Horn fly population dynamics as prediction tool for the fixation of pesticide resistance

    USDA-ARS?s Scientific Manuscript database

    This research project was conducted to establish the population dynamics of the horn fly. Two cattle herds were monitored to establish if contrasting climatic regional conditions, in addition to temperature and precipitation, related to the number of rainy days as a factor influencing horn fly infes...

  14. Rdl-containing Fragment of GABA(A) from the Horn Fly, Haematobia Irritans, Susceptible Genotype

    USDA-ARS?s Scientific Manuscript database

    The horn fly, Haematobia irritans (L.), is a significant economic pest of cattle found throughout Europe, North Africa, Asia Minor, and the Americas. The major means of controlling the horn fly is through applications of chemicals with insecticidal activity. A cyclodiene-containing ear tag product h...

  15. Pyrosequencing-based analysis of the microbiome associated with the horn fly, Haematobia irritans

    USDA-ARS?s Scientific Manuscript database

    The horn fly, Haematobia irritans, is one of the economically important pests of cattle. Use of insecticides have been a major element of horn fly management programs. Growing concerns of insecticide resistance, insecticide residues on farm products, and non-availability of new generation insecticid...

  16. Pyrosequencing-based Analysis of the Microbiome Associated with the Horn Fly, Haematobia Irritans

    USDA-ARS?s Scientific Manuscript database

    The horn fly, Haematobia irritans, is one of the economically important pests of cattle. Use of insecticide has been a major element of horn fly management programs. Growing concerns of insecticide resistance, insecticide residues on farm products, and nonavailability of new generation insecticides ...

  17. Determination of the Phase Centers of Millimeter-Wave Horn Antennas Using a Holographic Interference Technique

    NASA Astrophysics Data System (ADS)

    McAuley, Ian; Murphy, J. Anthony; McCarthy, Darragh; Gradziel, Marcin; Mahon, Ronan; O'Sullivan, Creidhe; Trappe, Neil

    2016-04-01

    In this paper, we discuss how a holographic interference technique can be applied in the experimental determination of the phase centers of non-standard horn antennas in the millimeter-waveband. The phase center is the point inside the horn from which the radiation appears to emanate when viewed from the far-field, and knowing its location is necessary for optimizing coupling efficiencies to quasi-optical systems. For non-standard horn designs, and other feed structures, the phase center may be difficult to reliably predict by simulation, in which case, before committing to antenna manufacture, there is a requirement for it to be determined experimentally. Although the phase center can be recovered by direct phase measurement of the far-field beam pattern, this usually involves expensive instrumentation such as a vector network analyzer for millimeter wave horn antennas. In this paper, we describe one inexpensive alternative, which is based on measuring the interference pattern in intensity between the radiation from the horn of interest and a reference beam derived from the same coherent source in an off-axis holography setup. The accuracy of the approach is improved by comparison with the interference pattern of a well-understood standard horn (such as a corrugated conical horn) in the same experimental setup. We present an example of the technique applied to a profiled smooth-walled horn antenna, which has been especially designed for cosmic microwave background (CMB) polarization experiments.

  18. Nutritional Limitation on Growth and Development of Horn Fly (Diptera:Muscidae) Larvae

    USDA-ARS?s Scientific Manuscript database

    Aging of bovine dung for 3-5 yr under refrigeration depleted nutrients required for growth and development of horn fly larvae. Growth of horn fly larvae in mixtures of nutrient-depleted dung and fresh dung resulted in production of larvae and pupae stunted in proportion to the relative amount of ag...

  19. USAFRICOM’s Role in Counter-Piracy Operations Within the Horn of Africa

    DTIC Science & Technology

    2009-05-04

    2008), 3. 45 “A Cooperative Strategy for 21 st Century Seapower”, October 2007. 46 Jacquelyn S. Porth , “Piracy Off the Horn of Africa Threatens...owens.piracy.html, (accessed 19 April 2009). Porth , Jacquelyn S. “Piracy Off the Horn of Africa Threatens Relief Efforts, Trade.” America.gov. http

  20. 76 FR 53295 - Unexpected Urgent Refugee and Migration Needs Related to the Horn of Africa

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-08-25

    ... Urgent Refugee and Migration Needs Related to the Horn of Africa Presidential Determination No. 2011-13... Africa Memorandum for the Secretary of State By the authority vested in me as President by the... humanitarian crisis in the Horn of Africa. You are authorized and directed to publish this memorandum in...

  1. Knemidokoptes mutans (Acari: Knemidocoptidae) in a great-horned owl (Bubo virginianus).

    PubMed

    Schulz, T A; Stewart, J S; Fowler, M E

    1989-07-01

    A routine examination of a captive juvenile great-horned owl (Bubo virginianus) revealed bilateral proliferative papillary hyperkeratosis on the feet. Microscopic examination of skin scrapings produced numerous mites identified as Knemidokoptes mutans. This is the first record of this parasite in a great-horned owl. A single dose of ivermectin (200 micrograms/kg) was effective in treatment of this infection.

  2. Identification of oxytocin receptor in the dorsal horn and nociceptive dorsal root ganglion neurons.

    PubMed

    Moreno-López, Y; Martínez-Lorenzana, G; Condés-Lara, M; Rojas-Piloni, G

    2013-04-01

    Oxytocin (OT) secreted by the hypothalamo-spinal projection exerts antinociceptive effects in the dorsal horn. Electrophysiological evidence indicates that OT could exert these effects by activating OT receptors (OTR) directly on dorsal horn neurons and/or primary nociceptive afferents in the dorsal root ganglia (DRG). However, little is known about the identity of the dorsal horn and DRG neurons that express the OTR. In the dorsal horn, we found that the OTR is expressed principally in neurons cell bodies. However, neither spino-thalamic dorsal horn neurons projecting to the contralateral thalamic ventral posterolateral nucleus (VPL) and posterior nuclear group (Po) nor GABaergic dorsal horn neurons express the OTR. The OTR is not expressed in skin nociceptive terminals or in dorsal horn nociceptive fibers. In the DRG, however, the OTR is expressed predominantly in non-peptidergic C-fiber cell bodies, but not in peptidergic or mechanoreceptor afferents or in skin nociceptive terminals. Our results suggest that the antinociceptive effects of OT are mediated by direct activation of dorsal horn neurons and peripheral actions on nociceptive, non-peptidergic C-afferents in the DRG.

  3. 49 CFR Appendix E to Part 222 - Requirements for Wayside Horns

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 49 Transportation 4 2010-10-01 2010-10-01 false Requirements for Wayside Horns E Appendix E to Part 222 Transportation Other Regulations Relating to Transportation (Continued) FEDERAL RAILROAD.... 222, App. E Appendix E to Part 222—Requirements for Wayside Horns This appendix sets forth...

  4. Milk production traits of beef cows as affected by horn fly count and sire breed type

    USDA-ARS?s Scientific Manuscript database

    Horn fly infestations on beef cattle results in decreased productivity and challenges enterprise sustainability. Objective of this experiment was to determine the relationships among, cattle breed, heat shock protein 70 (Hsp70) genotype, and horn fly density. Angus (n = 20), Brahman (n = 17), and ...

  5. Associations among heat shock protein 70 genotype, forage system, and horn fly infestation of beef cattle

    USDA-ARS?s Scientific Manuscript database

    Horn fly infestations negatively impact economic traits of beef cattle. The impact of horn flies on beef cattle milk yield and quality was evaluated in cows sired by Bonsmara (BONS; n = 7), Brangus (BRAN; n = 13), Charolais (CHAR; n = 8), Gelbvieh (GELV; n = 5), Hereford (HERF; n = 12), and Romosin...

  6. An Interview with Michael Horn: Blending Education for High-Octane Motivation

    ERIC Educational Resources Information Center

    Patterson, Gregory A.

    2012-01-01

    Blended learning holds the potential of improving the way we educate students and of making them more motivated. Blended education--the melding of information technology based distance learning with school attendance--is perhaps the best way to educate students for 21st century skills, says Michael Horn in a "Kappan" interview. Horn points out…

  7. 30 CFR 57.14132 - Horns and backup alarms for surface equipment.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... NONMETAL MINES Machinery and Equipment Safety Devices and Maintenance Requirements § 57.14132 Horns and backup alarms for surface equipment. (a) Manually-operated horns or other audible warning devices provided on self-propelled mobile equipment as a safety device shall be maintained in a functional...

  8. 30 CFR 57.14132 - Horns and backup alarms for surface equipment.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... NONMETAL MINES Machinery and Equipment Safety Devices and Maintenance Requirements § 57.14132 Horns and backup alarms for surface equipment. (a) Manually-operated horns or other audible warning devices provided on self-propelled mobile equipment as a safety device shall be maintained in a functional...

  9. Meniscectomy of horizontal tears of the lateral meniscus anterior horn using the joystick technique.

    PubMed

    Park, Ill Ho; Kim, Sung Jae; Choi, Duck Hyun; Lee, Su Chan; Park, Ha Young; Jung, Kwang Am

    2014-01-01

    Unstable inferior leaves of the anterior horn in horizontal tears of the lateral meniscus are challenging lesions for most orthopedic surgeons due to the poor viewing angle and the instability of these lesions. Resection of an exact volume is required for the successful treatment of horizontal tears in the lateral meniscus anterior horn. We report a method based on the joystick technique.

  10. 30 CFR 57.14132 - Horns and backup alarms for surface equipment.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... NONMETAL MINES Machinery and Equipment Safety Devices and Maintenance Requirements § 57.14132 Horns and backup alarms for surface equipment. (a) Manually-operated horns or other audible warning devices provided on self-propelled mobile equipment as a safety device shall be maintained in a functional...

  11. Offshore drilling, construction: Fortunes tied to stable gas prices

    SciTech Connect

    Pagano, S.S.; Marsh, T.

    1993-01-01

    Significantly improved US natural gas prices fueled an upswing in offshore drilling in the Gulf of Mexico in late 1992. Stabilized gas prices will be necessary to support both the off-shore drilling and construction markets in 1993 and beyond. The article discusses both these segments in detail: offshore drilling and offshore construction.

  12. 47 CFR 22.1037 - Application requirements for offshore stations.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... CARRIER SERVICES PUBLIC MOBILE SERVICES Offshore Radiotelephone Service § 22.1037 Application requirements for offshore stations. Applications for new Offshore Radiotelephone Service stations must contain an... 47 Telecommunication 2 2010-10-01 2010-10-01 false Application requirements for offshore stations...

  13. Offshore drilling to increase in 1984

    SciTech Connect

    Not Available

    1984-05-01

    A mid-year report presents a worldwide overview of offshore drilling operations. The Gulf of Mexico and the North Sea are posting substantial gains as companies rush to drill the millions of acres acquired in those sectors both last year and in previous offerings. The Middle East, Latin America and the Asia/Pacific offshore theaters will see only slight increases. The Mediterranean and African regions are expected to sustain declines. A region-by-region summary of the 1984 offshore forecast is presented showing exploration and appraisal activities, as well as development for all of these areas.

  14. Certification of offshore mooring steel wire ropes

    SciTech Connect

    Lohne, P.W.

    1996-12-31

    The trend to produce oil in increasingly deeper water has led to the development of floating production solutions for the exploitation of the energy resources in these areas. It is a fact that steel wire ropes have been used and are being proposed as line segments in the majority of the mooring systems of these units/ships. This paper specifies requirements for the materials, design, manufacture and testing of large diameter offshore mooring steel wire ropes and may serve as a technical reference document in contractual matters between the purchaser and the manufacturer. Typical applications covered are permanently moored floating production systems (FPS), offshore loading systems and mobile offshore units.

  15. Current and future offshore activities in Canada

    SciTech Connect

    Hnatiuk, J.

    1987-06-01

    The development of innovative exploratory drilling systems for Canada's harsh Arctic offshore areas over the past decade and future activity in these areas, including possible production concepts, are discussed. The results can be applied in other Arctic areas of the world, including offshore Alaska. This operating experience will advance technology and serve as a basis for the design of Arctic offshore production and transportation systems. Unique technology has been developed and successfully used in the discovery of major accumulations of hyrodcarbons. Continued technological advances are anticipated to have widespread Arctic applications in both exploratory and production operations.

  16. Evaluation of alternative tactics for management of insecticide-resistant horn flies (Diptera: Muscidae).

    PubMed

    Steelman, C D; McNew, R W; Simpson, R B; Rorie, R W; Phillips, J M; Rosenkrans, C F

    2003-06-01

    A 3-yr study was conducted to determine the efficacy of tactics that could be used to manage populations of insecticide-resistant horn flies, Hematobia irritans irritans (L.). Insecticide spray, spot-on or pour-on formulations and two IGRs in bolus formulation, 1.3- and 3.2-ha pasture rotations on different rotation schedules, 0-50% Brahman breeding, selected fly-resistant cows, and a mechanical trap were evaluated singly and in combination. Concentration-mortality tests indicated that horn flies collected from cows used in the current study were significantly less susceptible to diazinon, coumaphos, and methoxychlor than horn flies from cows at the same locations previously used to determine baseline susceptibility. During the 3-yr study at the Southeast Research and Extension Center (SEREC), the IGR-bolus significantly reduced (P < 0.05) horn fly numbers on both the continuous and rotational graze regimens, resulting in significantly (P < 0.05) greater calf weaning weights (average of 24 kg). Horn fly numbers were significantly greater on untreated cows during the 3-yr study at the Southwest Research and Extension Center (SWREC) compared with the mean fly numbers on cows that received fly-management treatments. All tactics and tactic-combinations used at SWREC on cattle having no Brahman breeding failed to significantly reduce insecticide-resistant horn fly numbers. However, the combination of Brahman breeding with the IGR-Bolus and mechanical trap significantly reduced horn fly numbers and resulted in significant increases in calf weaning weight. In addition, mean horn fly numbers decreased significantly as the percentage Brahman breeding increased with 50% Brahman breeding reducing horn fly numbers by 140 flies per cow. No significant difference was found between the mean fly numbers on the fly-resistant purebred group and the cows that had no Brahman breeding but received the IGR-Bolus or used the mechanical trap. The use of synergized zeta-cypermethrin pour

  17. High-gain step-profiled integrated diagonal horn-antennas

    NASA Astrophysics Data System (ADS)

    Eleftheriades, George V.; Rebeiz, Gabriel M.

    1992-05-01

    A new step-profiled integrated-horn antenna is proposed. The antenna allows gains in the range of 17-20 dB to be achieved using standard (100) silicon wafers. The antenna is diagonally fed and exhibits very good circular symmetry within the 10-dB beamwidth. It has a fundamental Gaussian coupling efficiency of 83 percent. It is demonstrated that the profiled antenna has a radiation pattern similar to that of its smooth envelope horn, provided that the discontinuity between successive wafers does not exceed 0.15 lambda. The integrated stepped-profile horn performs much better than a corresponding smooth 70 deg flare-angle integrated horn of the same aperture size. The integrated step-profile horn is very well-suited for radio-astonomical and remote-sensing millimeter-wave imaging arrays requiring a large number of focal-plane elements.

  18. Pangolin expression influences the development of a morphological novelty: beetle horns.

    PubMed

    Wasik, Bethany R; Moczek, Armin P

    2012-05-01

    Morphological diversity arises during development through the actions and interactions of diverse developmental pathways. Among those, the Wnt pathway is known to contribute to diverse developmental processes such as segmentation and the morphogenesis of appendages. Here, we characterize a transcription factor in the Wnt pathway, pangolin (pan), to investigate the role of Wnt signaling in the development of evolutionarily novel body structures: the horns of beetles. Beetle horns are highly diverse in size, shape, and number and develop principally from two major body regions: the head and prothorax. We investigate horns in two species of the genus Onthophagus using comparative in situ hybridization, larval RNA interference, and allometric measurements to analyze whether horn formation is regulated by pan and by extension the Wnt pathway. Our results illustrate that pan expression affects beetle horn growth in a species-, sex-, and location-specific manner in two morphologically distinct, yet closely-related, Onthophagus species.

  19. Cutaneous horn arising from an area of discoid lupus erythematosus on the scalp.

    PubMed

    Fatani, Mohammad Ibrahim; Hussain, Waleed Mohd; Baltow, Badee; Alsharif, Sahar

    2014-04-03

    A cutaneous horn is a rare clinical condition characterised by a conical projection of hyperkeratotic epidermis. Cutaneous horns most commonly arise from sun-exposed skin in elderly men, but may arise from any part of the body at any age in men and women. When a cutaneous horn forms, it is important to determine the underlying cause. Various skin diseases may present with cutaneous horns including viral warts, actinic keratosis, keratoacanthoma, seborrhoeic keratosis, pyogenic granuloma, discoid lupus erythematosus, verruca vulgaris, Bowen's disease, basal cell carcinoma and squamous cell carcinoma. The underlying pathology is benign in 61.1% of cases, premalignant in 23.2% of cases and malignant in 15.7% of cases. We report a patient with a cutaneous horn arising from an area of discoid lupus erythematosus on the scalp.

  20. pangolin expression influences the development of a morphological novelty: beetle horns

    PubMed Central

    Wasik, Bethany R.; Moczek, Armin P.

    2011-01-01

    Morphological diversity arises during development through the actions and interactions of diverse developmental pathways. Among those, the Wnt pathway is known to contribute to diverse developmental processes such as segmentation and the morphogenesis of appendages. Here, we characterize a transcription factor in the Wnt pathway, pangolin (pan), to investigate the role of Wnt signaling in the development of evolutionarily novel body structures: the horns of beetles. Beetle horns are highly diverse in size, shape and number and develop principally from two major body regions: the head and prothorax. We investigate horns in two species of the genus Onthophagus using comparative in situ hybridization, larval RNA interference, and allometric measurements to analyze whether horn formation is regulated by pan and by extension the Wnt pathway. Our results illustrate that pan expression affects beetle horn growth in a species-, sex-, and location-specific manner in two morphologically distinct, yet closely-related, Onthophagus species. PMID:21998033

  1. Preference of redear sunfish on zebra mussels and rams-horn snails

    USGS Publications Warehouse

    French, John R. P.; Morgan, Michael N.

    1995-01-01

    We tested prey preferences of adult (200- to 222-mm long) redear sunfish (Lepomis microlophus) on two size classes of zebra mussels (Dreissena polymorpha) and two-ridge rams-horns (Helisoma anceps) in experimental aquaria. We also tested physical limitations on consuming these mollusks and determined prey bioenergetic profitability. Redear sunfish strongly preferred rams-horns over zebra mussels, but they displayed no size preference for either prey. Ingestion was not physically limited since both prey species up to 15-mm long fit within the pharyngeal gapes of redear sunfish. Rams-horns were more bioenergetically profitable than zebra mussels and ingestion of rams-horn shell fragments was about three times less than zebra mussels. Rams-horns were somewhat more resistant to shell-crushing, but all size ranges of both prey species tested were crushable by redear sunfish. These studies suggested that the redear sunfish should not be considered a panacea for biological control of zebra mussels.

  2. Cutaneous horn arising from an area of discoid lupus erythematosus on the scalp

    PubMed Central

    Fatani, Mohammad Ibrahim; Hussain, Waleed Mohd; Baltow, Badee; Alsharif, Sahar

    2014-01-01

    A cutaneous horn is a rare clinical condition characterised by a conical projection of hyperkeratotic epidermis. Cutaneous horns most commonly arise from sun-exposed skin in elderly men, but may arise from any part of the body at any age in men and women. When a cutaneous horn forms, it is important to determine the underlying cause. Various skin diseases may present with cutaneous horns including viral warts, actinic keratosis, keratoacanthoma, seborrhoeic keratosis, pyogenic granuloma, discoid lupus erythematosus, verruca vulgaris, Bowen's disease, basal cell carcinoma and squamous cell carcinoma. The underlying pathology is benign in 61.1% of cases, premalignant in 23.2% of cases and malignant in 15.7% of cases. We report a patient with a cutaneous horn arising from an area of discoid lupus erythematosus on the scalp. PMID:24700037

  3. Design of highly uniform spool and bar horns for ultrasonic bonding.

    PubMed

    Kim, Sun-Rak; Lee, Jae Hak; Yoo, Choong D; Song, Jun-Yeob; Lee, Seung S

    2011-10-01

    Although the groove and slot have been widely utilized for horn design to achieve high uniformity, their effects on uniformity have not been analyzed thoroughly. In this work, spool and bar horns for ultrasonic bonding are designed in a systematic way using the design of experiments (DOE) to achieve high amplitude uniformity of the horn. Three-dimensional modal analysis is conducted to predict the natural frequency, amplitude, and stress of the horns, and the DOE is employed to analyze the effects of the groove and slot on the amplitude uniformity. The design equations are formulated to determine the optimum dimensions of the groove and slot, and the uniformity is found to be influenced most significantly by the groove depth and slot width. Displacements of the spool and bar horns were measured using a laser Doppler vibrometer (LDV), and the predicted results are in good agreement with the experimental data.

  4. The development of an L-band radiometer dual-mode horn. [for ocean surface temperature measurement

    NASA Technical Reports Server (NTRS)

    Bailey, M. C.

    1975-01-01

    An antenna was developed for the remote microwave measurement of ocean surface temperature during a flight test in a C54 aircraft. The basic antenna is a conical dual-mode horn similar to the dual-mode horn described by Potter (1963). The pertinent internal dimensions of the horn are given. The measured E and H plane patterns for the linearly polarized horn for a range of frequencies are shown in a graph.

  5. Production of avian leukosis virus particles in mammalian cells can be mediated by the interaction of the human immunodeficiency virus protein Rev and the Rev-responsive element.

    PubMed Central

    Nasioulas, G; Hughes, S H; Felber, B K; Whitcomb, J M

    1995-01-01

    In human immunodeficiency virus type 1-infected cells, the efficient expression of viral proteins from unspliced and singly spliced RNAs is dependent on two factors: the presence in the cell of the viral protein Rev and the presence in the viral RNA of the Rev-responsive element (RRE). We show here that the HIV-1 Rev/RRE system can increase the expression of avian leukosis virus (ALV) structural proteins in mammalian cells (D-17 canine osteosarcoma) and promote the release of mature ALV virions from these cells. In this system, the Rev/RRE interaction appears to facilitate the export of full-length unspliced ALV RNA from the nucleus to the cytoplasm, allowing increased production of the ALV structural proteins. Gag protein is produced in the cytoplasm of the ALV-transfected cells even in the absence of a Rev/RRE interaction. However, a functional Rev/RRE interaction increases the amount of Gag present intracellularly and, more strikingly, results in the release of mature ALV particles into the supernatant. RCAS virus containing an RRE is replication-competent in chicken embryo fibroblasts; however, we have been unable to determine whether the particles produced in D-17 cells are as infectious as the particles produced in chicken embryo fibroblasts. Images Fig. 2 Fig. 3 Fig. 4 PMID:8524879

  6. The DNA polymerase activity of Saccharomyces cerevisiae Rev1 is biologically significant.

    PubMed

    Wiltrout, Mary Ellen; Walker, Graham C

    2011-01-01

    A cell's ability to tolerate DNA damage is directly connected to the human development of diseases and cancer. To better understand the processes underlying mutagenesis, we studied the cell's reliance on the potentially error-prone translesion synthesis (TLS), and an error-free, template-switching pathway in Saccharomyces cerevisiae. The primary proteins mediating S. cerevisiae TLS are three DNA polymerases (Pols): Rev1, Pol ζ (Rev3/7), and Pol η (Rad30), all with human homologs. Rev1's noncatalytic role in recruiting other DNA polymerases is known to be important for TLS. However, the biological significance of Rev1's unusual conserved DNA polymerase activity, which inserts dC, is much less well understood. Here, we demonstrate that inactivating Rev1's DNA polymerase function sensitizes cells to both chronic and acute exposure to 4-nitroquinoline-1-oxide (4-NQO) but not to UV or cisplatin. Full Rev1-dependent resistance to 4-NQO, however, also requires the additional Rev1 functions. When error-free tolerance is disrupted through deletion of MMS2, Rev1's catalytic activity is more vital for 4-NQO resistance, possibly explaining why the biological significance of Rev1's catalytic activity has been elusive. In the presence or absence of Mms2-dependent error-free tolerance, the catalytic dead strain of Rev1 exhibits a lower 4-NQO-induced mutation frequency than wild type. Furthermore, Pol ζ, but not Pol η, also contributes to 4-NQO resistance. These results show that Rev1's catalytic activity is important in vivo when the cell has to cope with specific DNA lesions, such as N(2)-dG.

  7. A Cell-penetrating Antibody Fragment against HIV-1 Rev Has High Antiviral Activity

    PubMed Central

    Zhuang, Xiaolei; Stahl, Stephen J.; Watts, Norman R.; DiMattia, Michael A.; Steven, Alasdair C.; Wingfield, Paul T.

    2014-01-01

    The HIV-1 protein Rev oligomerizes on viral transcripts and directs their nuclear export. Previously, a Fab against Rev generated by phage display was used to crystallize and solve the structure of the Rev oligomerization domain. Here we have investigated the capability of this Fab to block Rev oligomerization and inhibit HIV-1 replication. The Fab itself did not have antiviral activity, but when a Tat-derived cell-penetrating peptide was appended, the resulting molecule (FabRev1-Tat) was strongly inhibitory of three different CCR5-tropic HIV-1 isolates (IC50 = 0.09–0.44 μg/ml), as assessed by suppression of reverse transcriptase activity in infected peripheral blood mononuclear cells, and had low cell toxicity (TC50 > 100 μg/ml). FabRev1-Tat was taken up by both peripheral blood mononuclear and HEK293T cells, appearing in both the cytoplasm and nucleus, as shown by immunofluorescence confocal laser scanning microscopy. Computational alanine scanning was used to identify key residues in the complementarity-determining regions to guide mutagenesis experiments. Residues in the light chain CDR3 (LCDR3) were assessed to be important. Residues in LCDR3 were mutated, and LCDR3-Tyr92 was found to be critical for binding to Rev, as judged by surface plasmon resonance and electron microscopy. Peptides corresponding to all six CDR regions were synthesized and tested for Rev binding. None of the linear peptides had significant affinity for Rev, but four of the amide-cyclic forms did. Especially cyclic-LCDR3 (LGGYPAASYRTA) had high affinity for Rev and was able to effectively depolymerize Rev filaments, as shown by both surface plasmon resonance and electron microscopy. PMID:24878961

  8. H-rev107 Regulates Cytochrome P450 Reductase Activity and Increases Lipid Accumulation

    PubMed Central

    Tsai, Fu-Ming; Chen, Mao-Liang; Wang, Lu-Kai; Lee, Ming-Cheng

    2015-01-01

    H-rev107 is a member of the HREV107 type II tumor suppressor gene family and acts as a phospholipase to catalyze the release of fatty acids from glycerophospholipid. H-rev107 has been shown to play an important role in fat metabolism in adipocytes through the PGE2/cAMP pathway, but the detailed molecular mechanism underlying H-rev107-mediated lipid degradation has not been studied. In this study, the interaction between H-rev107 and cytochrome P450 reductase (POR), which is involved in hepatic lipid content regulation, was determined by yeast two-hybrid screen and confirmed by using in vitro pull down assays and immunofluorescent staining. The expression of POR in H-rev107-expressing cells enhanced the H-rev107-mediated release of arachidonic acid. However, H-rev107 inhibited POR activity and relieved POR-mediated decreased triglyceride content in HtTA and HeLa cervical cells. The inhibitory effect of H-rev107 will be abolished when POR-expressing cells transfected with PLA2-lacking pH-rev107 or treated with PLA2 inhibitor. Silencing of H-rev107 using siRNA resulted in increased glycerol production and reversion of free fatty acid-mediated growth suppression in Huh7 hepatic cells. In summary, our results revealed that H-rev107 is also involved in lipid accumulation in liver cells through the POR pathway via its PLA2 activity. PMID:26381418

  9. Diversification of doublesex function underlies morph-, sex-, and species-specific development of beetle horns

    PubMed Central

    Kijimoto, Teiya; Moczek, Armin P.; Andrews, Justen

    2012-01-01

    Sex-specific trait expression is frequently associated with highly variable, condition-dependent expression within sexes and rapid divergence among closely related species. Horned beetles are an excellent example for studying the molecular basis of these phenomena because horn morphology varies markedly among species, between sexes, and among alternative, nutritionally-cued morphs within sexes. In addition, horns lack obvious homology to other insect traits and provide a good opportunity to explore the molecular basis of the rapid diversification of a novel trait within and between species. Here we show that the sex-determination gene doublesex (dsx) underlies important aspects of horn development, including differences between sexes, morphs, and species. In male Onthophagus taurus, dsx transcripts were preferentially expressed in the horns of the large, horned morph, and RNAi-mediated knockdown of dsx dramatically altered male horn allometry by massively reducing horn development in large males, but not in smaller males. Conversely, dsx RNAi induced ectopic, nutrition-sensitive horn development in otherwise hornless females. Finally, in a closely related species (Onthophagus sagittarius) that has recently evolved a rare reversed sexual dimorphism, dsx RNAi revealed reversed as well as novel dsx functions despite an overall conservation of dsx expression. This suggests that rapid evolution of dsx functions has facilitated the transition from a regular sexual dimorphism to a reversed sexual dimorphism in this species. Our findings add beetle horns to existing examples of a close relationship between dsx and sexual trait development, and suggest that dsx function has been coopted to facilitate both the evolution of environmentally-cued intrasexual dimorphisms and rapid species divergences in a novel trait. PMID:23184999

  10. 75 FR 9377 - Endangered and Threatened Wildlife and Plants; Listing the Flat-Tailed Horned Lizard as Threatened

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-03-02

    ... Flat- Tailed Horned Lizard as Threatened AGENCY: Fish and Wildlife Service, Interior. ACTION..., proposed rule to list the flat-tailed horned lizard (Phrynosoma mcallii) as threatened under the Endangered..., 1993 (58 FR 62624), to list the flat-tailed horned lizard as a threatened species, and reopens the...

  11. 9 CFR 310.9 - Anthrax; carcasses not to be eviscerated; disposition of affected carcasses; hides, hoofs, horns...

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... eviscerated; disposition of affected carcasses; hides, hoofs, horns, hair, viscera and contents, and fat...; hides, hoofs, horns, hair, viscera and contents, and fat; handling of blood and scalding vat water..., hoofs, horns, hair, viscera and contents, blood, and fat of any livestock found to be affected...

  12. Multiplex Polymerase Chain Reaction in the Horn Fly: Detection of Pyrethroid, Organophosphate and Cyclodiene Target Site Resistance

    USDA-ARS?s Scientific Manuscript database

    The horn fly, Haematobia irritans irritans, is an important pest to the livestock industry that causes economic losses of approximately US$1 billion in the U.S. and a similar value in Latin America. Horn fly control efforts still relies mainly on direct application of insecticides although horn fly ...

  13. 49 CFR 222.23 - How does this regulation affect sounding of a horn during an emergency or other situations?

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... other provision of this part, a locomotive engineer may sound the locomotive horn to provide a warning... imminent injury, death, or property damage. (2) Notwithstanding any other provision of this part, including... locomotive horns in emergency situations, nor does it impose a legal duty to sound the locomotive horn...

  14. Offshore oil in the Alaskan Arctic

    NASA Technical Reports Server (NTRS)

    Weeks, W. F.; Weller, G.

    1984-01-01

    Oil and gas deposits in the Alaskan Arctic are estimated to contain up to 40 percent of the remaining undiscovered crude oil and oil-equivalent natural gas within U.S. jurisdiction. Most (65 to 70 percent) of these estimated reserves are believed to occuur offshore beneath the shallow, ice-covered seas of the Alaskan continental shelf. Offshore recovery operations for such areas are far from routine, with the primary problems associated with the presence of ice. Some problems that must be resolved if efficient, cost-effective, environmentally safe, year-round offshore production is to be achieved include the accurate estimation of ice forces on offshore structures, the proper placement of pipelines beneath ice-produced gouges in the sea floor, and the cleanup of oil spills in pack ice areas.

  15. Offshore oil in the alaskan arctic.

    PubMed

    Weeks, W F; Weller, G

    1984-07-27

    Oil and gas deposits in the Alaskan Arctic are estimated to contain up to 40 percent of the remaining undiscovered crude oil and oil-equivalent nature gas within U.S. jurisdiction. Most (65 to 70 percent) of these estimated reserves are believed to occur offshore beneath the shallow, ice-covered seas, of the Alaskan continental shelf. Offshore recovery operations for such areas are far from routine, with the primary problems associated with the presence of ice. Some problems that must be resolved if efficient, cost-effective, environmentally safe, year-round offshore production is to be achieved include the accurate estimation of ice forces on offshore structures, the proper placement of pipelines beneath ice-produced gouges in the sea floor, and the cleanup of oil spills in pack ice areas.

  16. EMS offshore. A new horizon for paramedics.

    PubMed

    Mallard, A S

    1991-10-01

    The difficulty in getting medical aid to offshore drilling platforms can be a source of life-threatening delays. Recently, some companies have charted new waters by actually stationing EMS crews on their rigs.

  17. Civil engineering in the Arctic offshore

    SciTech Connect

    Bennett, F.L.; Machemehl, J.L.

    1985-01-01

    This book presents the current state of practice and theory in the civil engineering aspects of offshore development in the Arctic. It also covers the emerging concepts and requirements, research and development needs, and a critique of present undergraduate programmes.

  18. Offshore oil in the Alaskan Arctic

    NASA Technical Reports Server (NTRS)

    Weeks, W. F.; Weller, G.

    1984-01-01

    Oil and gas deposits in the Alaskan Arctic are estimated to contain up to 40 percent of the remaining undiscovered crude oil and oil-equivalent natural gas within U.S. jurisdiction. Most (65 to 70 percent) of these estimated reserves are believed to occuur offshore beneath the shallow, ice-covered seas of the Alaskan continental shelf. Offshore recovery operations for such areas are far from routine, with the primary problems associated with the presence of ice. Some problems that must be resolved if efficient, cost-effective, environmentally safe, year-round offshore production is to be achieved include the accurate estimation of ice forces on offshore structures, the proper placement of pipelines beneath ice-produced gouges in the sea floor, and the cleanup of oil spills in pack ice areas.

  19. Engineering Challenges for Floating Offshore Wind Turbines

    SciTech Connect

    Butterfield, S.; Musial, W.; Jonkman, J.; Sclavounos, P.

    2007-09-01

    The major objective of this paper is to survey the technical challenges that must be overcome to develop deepwater offshore wind energy technologies and to provide a framework from which the first-order economics can be assessed.

  20. New perspectives in offshore wind energy.

    PubMed

    Failla, Giuseppe; Arena, Felice

    2015-02-28

    The design of offshore wind turbines is one of the most fascinating challenges in renewable energy. Meeting the objective of increasing power production with reduced installation and maintenance costs requires a multi-disciplinary approach, bringing together expertise in different fields of engineering. The purpose of this theme issue is to offer a broad perspective on some crucial aspects of offshore wind turbines design, discussing the state of the art and presenting recent theoretical and experimental studies.

  1. New perspectives in offshore wind energy

    PubMed Central

    Failla, Giuseppe; Arena, Felice

    2015-01-01

    The design of offshore wind turbines is one of the most fascinating challenges in renewable energy. Meeting the objective of increasing power production with reduced installation and maintenance costs requires a multi-disciplinary approach, bringing together expertise in different fields of engineering. The purpose of this theme issue is to offer a broad perspective on some crucial aspects of offshore wind turbines design, discussing the state of the art and presenting recent theoretical and experimental studies. PMID:25583869

  2. Arctic offshore drilling: a new challenge

    SciTech Connect

    Wetmore, S.B.

    1985-11-01

    The arctic offshore environment provides the drilling industry with its greatest challenge yet. Problems due to sea ice, high wind, extreme cold and poor bottom soil have required the design of special structures appropriate only for arctic offshore drilling. The challenge lies not just in the obvious factors of temperature and ice, but also the basic physics of ice problems are not well understood. The arctic environment and the petroleum industry are discussed.

  3. 2014 Offshore Wind Market and Economic Analysis

    SciTech Connect

    Hamilton, Bruce

    2014-08-25

    The objective of this report is to provide a comprehensive annual assessment of the U.S. offshore wind market.This 3rd annual report focuses on new developments that have occurred in 2014. The report provides stakeholders with a reliable and consistent data source addressing entry barriers and U.S. competitiveness in the offshore wind market. Available for download are both the full report and the report's underlying data.

  4. Pharmacological and Genetic Modulation of REV-ERB Activity and Expression Affects Orexigenic Gene Expression

    PubMed Central

    Amador, Ariadna; Wang, Yongjun; Banerjee, Subhashis; Kameneka, Theodore M.; Solt, Laura A.; Burris, Thomas P.

    2016-01-01

    The nuclear receptors REV-ERBα and REV-ERBβ are transcription factors that play pivotal roles in the regulation of the circadian rhythm and various metabolic processes. The circadian rhythm is an endogenous mechanism, which generates entrainable biological changes that follow a 24-hour period. It regulates a number of physiological processes, including sleep/wakeful cycles and feeding behaviors. We recently demonstrated that REV-ERB-specific small molecules affect sleep and anxiety. The orexinergic system also plays a significant role in mammalian physiology and behavior, including the regulation of sleep and food intake. Importantly, orexin genes are expressed in a circadian manner. Given these overlaps in function and circadian expression, we wanted to determine whether the REV-ERBs might regulate orexin. We found that acute in vivo modulation of REV-ERB activity, with the REV-ERB-specific synthetic ligand SR9009, affects the circadian expression of orexinergic genes in mice. Long term dosing with SR9009 also suppresses orexinergic gene expression in mice. Finally, REV-ERBβ-deficient mice present with increased orexinergic transcripts. These data suggest that the REV-ERBs may be involved in the repression of orexinergic gene expression. PMID:26963516

  5. Biological Characterization of CVRM2-BAC, A Recombinant CV1988 Virus Containing an REV LTR Insertion

    USDA-ARS?s Scientific Manuscript database

    It has been previously reported that avian retroviruses, i.e. avian leukosis virus (ALV) and reticoloendotheliosis virus (REV), integrate in the Marek’s disease virus genome affecting MDV pathogenicity. RM-2 is an attenuated serotype 1 MDV virus generated by insertion of the REV LTR in the genome of...

  6. The nuclear receptor and clock gene REV-ERBα regulates cigarette smoke-induced lung inflammation.

    PubMed

    Sundar, Isaac K; Rashid, Kahkashan; Sellix, Michael T; Rahman, Irfan

    2017-09-30

    REV-ERBα is a nuclear heme receptor, transcriptional repressor and critical component of the molecular clock that drives daily rhythms of metabolism. Evidence reveals that REV-ERBα also plays an important regulatory role in clock-dependent lung physiology and inflammatory responses. We hypothesize that cigarette smoke (CS) exposure influences REV-ERBα abundance in the lungs, facilitating a pro-inflammatory phenotype. To determine the impact of REV-ERBα activation in the CS-induced inflammatory response we treated primary human small airway epithelial cells (SAECs) with CS extract (CSE) or lipopolysaccharide (LPS) in the absence or presence of pre-treatment with the REV-ERBα agonist GSK 4112. We also exposed adult C57BL/6J (WT) and Rev-erbα global KO mice to CS (10 and 30 days) and measured pro-inflammatory cytokine release. Our data reveal that pre-treatment with GSK 4112 reduced CSE/LPS induced pro-inflammatory cytokines release from both SAECs and mouse lung fibroblasts (MLFs). Furthermore, REV-ERBα KO mice show a greater inflammatory response to 10 and 30 days of CS, including increased neutrophil lung influx, pro-inflammatory cytokine (IL-6, MCP-1 and KC) release, and pro-senescence marker (p16) when compared to WT mice. These data demonstrate that REV-ERBα is a critical regulator of CS-induced lung inflammatory responses. Copyright © 2017. Published by Elsevier Inc.

  7. Molecular interaction between K-Ras and H-REV107 in the Ras signaling pathway.

    PubMed

    Han, Chang Woo; Jeong, Mi Suk; Jang, Se Bok

    2017-09-16

    Ras proteins are small GTPases that serve as master moderators of a large number of signaling pathways involved in various cellular processes. Activating mutations in Ras are found in about one-third of cancers. H-REV107, a K-Ras binding protein, plays an important role in determining K-Ras function. H-REV107 is a member of the HREV107 family of class II tumor suppressor genes and a growth inhibitory Ras target gene that suppresses cellular growth, differentiation, and apoptosis. Expression of H-REV107 was strongly reduced in about 50% of human carcinoma cell lines. However, the specific molecular mechanism by which H-REV107 inhibits Ras is still unknown. In the present study, we suggest that H-REV107 forms a strong complex with activating oncogenic mutation Q61H K-Ras from various biochemical binding assays and modeled structures. In addition, the interaction sites between K-Ras and H-REV107 were predicted based on homology modeling. Here, we found that some structure-based mutants of the K-Ras disrupted the complex formation with H-REV107. Finally, a novel molecular mechanism describing K-Ras and H-REV107 binding is suggested and insights into new K-Ras effector target drugs are provided. Copyright © 2017 Elsevier Inc. All rights reserved.

  8. 48 CFR 53.301-18 - SF 18 (Rev. 6/95), Request for Quotations.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 48 Federal Acquisition Regulations System 2 2010-10-01 2010-10-01 false SF 18 (Rev. 6/95), Request for Quotations. 53.301-18 Section 53.301-18 Federal Acquisition Regulations System FEDERAL ACQUISITION REGULATION (CONTINUED) CLAUSES AND FORMS FORMS Illustrations of Forms 53.301-18 SF 18 (Rev. 6/95),...

  9. 48 CFR 53.301-18 - SF 18 (Rev. 6/95), Request for Quotations.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 48 Federal Acquisition Regulations System 2 2012-10-01 2012-10-01 false SF 18 (Rev. 6/95), Request for Quotations. 53.301-18 Section 53.301-18 Federal Acquisition Regulations System FEDERAL ACQUISITION REGULATION (CONTINUED) CLAUSES AND FORMS FORMS Illustrations of Forms 53.301-18 SF 18 (Rev. 6/95),...

  10. 48 CFR 53.301-1414 - SF 1414 (Rev. 10/93) Consent of Surety.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 48 Federal Acquisition Regulations System 2 2011-10-01 2011-10-01 false SF 1414 (Rev. 10/93) Consent of Surety. 53.301-1414 Section 53.301-1414 Federal Acquisition Regulations System FEDERAL ACQUISITION REGULATION (CONTINUED) CLAUSES AND FORMS FORMS Illustrations of Forms 53.301-1414 SF 1414 (Rev....

  11. 48 CFR 53.301-18 - SF 18 (Rev. 6/95), Request for Quotations.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 48 Federal Acquisition Regulations System 2 2013-10-01 2013-10-01 false SF 18 (Rev. 6/95), Request for Quotations. 53.301-18 Section 53.301-18 Federal Acquisition Regulations System FEDERAL ACQUISITION REGULATION (CONTINUED) CLAUSES AND FORMS FORMS Illustrations of Forms 53.301-18 SF 18 (Rev. 6/95),...

  12. 48 CFR 53.301-18 - SF 18 (Rev. 6/95), Request for Quotations.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 48 Federal Acquisition Regulations System 2 2011-10-01 2011-10-01 false SF 18 (Rev. 6/95), Request for Quotations. 53.301-18 Section 53.301-18 Federal Acquisition Regulations System FEDERAL ACQUISITION REGULATION (CONTINUED) CLAUSES AND FORMS FORMS Illustrations of Forms 53.301-18 SF 18 (Rev. 6/95),...

  13. 48 CFR 53.301-18 - SF 18 (Rev. 6/95), Request for Quotations.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 48 Federal Acquisition Regulations System 2 2014-10-01 2014-10-01 false SF 18 (Rev. 6/95), Request for Quotations. 53.301-18 Section 53.301-18 Federal Acquisition Regulations System FEDERAL ACQUISITION REGULATION (CONTINUED) CLAUSES AND FORMS FORMS Illustrations of Forms 53.301-18 SF 18 (Rev. 6/95),...

  14. Rev-erb-α modulates skeletal muscle oxidative capacity by regulating mitochondrial biogenesis and autophagy

    PubMed Central

    Woldt, Estelle; Sebti, Yasmine; Solt, Laura A.; Duhem, Christian; Lancel, Steve; Eeckhoute, Jérôme; Hesselink, Matthijs K.C.; Paquet, Charlotte; Delhaye, Stéphane; Shin, Youseung; Kamenecka, Theodore M.; Schaart, Gert; Lefebvre, Philippe; Nevière, Rémi; Burris, Thomas P.; Schrauwen, Patrick; Staels, Bart; Duez, Hélène

    2013-01-01

    The nuclear receptor Rev-erb-α modulates hepatic lipid and glucose metabolism, adipogenesis and the inflammatory response in macrophages. We show here that Rev-erb-α is highly expressed in oxidative skeletal muscle and plays a role in mitochondrial biogenesis and oxidative function, in gain- and loss-of function studies. Rev-erb-α-deficiency in skeletal muscle leads to reduced mitochondrial content and oxidative function, resulting in compromised exercise capacity. This phenotype was recapitulated in isolated fibers and in muscle cells upon Rev-erbα knock-down, while Rev-erb-α over-expression increased the number of mitochondria with improved respiratory capacity. Rev-erb-α-deficiency resulted in deactivation of the Stk11–Ampk–Sirt1–Ppargc1-α signaling pathway, whereas autophagy was up-regulated, resulting in both impaired mitochondrial biogenesis and increased clearance. Muscle over-expression or pharmacological activation of Rev-erb-α increased respiration and exercise capacity. This study identifies Rev-erb-α as a pharmacological target which improves muscle oxidative function by modulating gene networks controlling mitochondrial number and function. PMID:23852339

  15. The Nuclear Receptor Rev-erbα Regulates Adipose Tissue-specific FGF21 Signaling.

    PubMed

    Jager, Jennifer; Wang, Fenfen; Fang, Bin; Lim, Hee-Woong; Peed, Lindsey C; Steger, David J; Won, Kyoung-Jae; Kharitonenkov, Alexei; Adams, Andrew C; Lazar, Mitchell A

    2016-05-13

    FGF21 is an atypical member of the FGF family that functions as a hormone to regulate carbohydrate and lipid metabolism. Here we demonstrate that the actions of FGF21 in mouse adipose tissue, but not in liver, are modulated by the nuclear receptor Rev-erbα, a potent transcriptional repressor. Interrogation of genes induced in the absence of Rev-erbα for Rev-erbα-binding sites identified βKlotho, an essential coreceptor for FGF21, as a direct target gene of Rev-erbα in white adipose tissue but not liver. Rev-erbα ablation led to the robust elevated expression of βKlotho. Consequently, the effects of FGF21 were markedly enhanced in the white adipose tissue of mice lacking Rev-erbα. A major Rev-erbα-controlled enhancer at the Klb locus was also bound by the adipocytic transcription factor peroxisome proliferator-activated receptor (PPAR) γ, which regulates its activity in the opposite direction. These findings establish Rev-erbα as a specific modulator of FGF21 signaling in adipose tissue. © 2016 by The American Society for Biochemistry and Molecular Biology, Inc.

  16. [Musculoskeletal disorders in the offshore oil industry].

    PubMed

    Morken, Tone; Tveito, Torill H; Torp, Steffen; Bakke, Ashild

    2004-10-21

    Musculoskeletal disorders are important causes of sick leave and disability among Norwegian offshore petroleum workers. More knowledge and interventions are needed in order to prevent this. In this review we consider prevalence and risk factors among offshore petroleum workers and point to the need for more research. Literature searches on ISI Web of Science and PubMed were supplemented by reports from Norwegian offshore industry companies and the Norwegian Petroleum Directorate. Few studies were found on musculoskeletal disorders among offshore petroleum workers. The disorders are widespread, particularly among catering, construction and drilling personnel. It is not clear whether the prevalence is different from that among onshore workers. Risk factors are physical stressors and fast pace of work. Among catering personnel, these disorders are important causes of loss of the required health certificate but we could not identify any review of causes in the offshore industry generally. More scientific studies are needed on musculoskeletal disorders as comparisons of prevalence and risk factors for offshore and onshore workers may point to more effective interventions. Better knowledge of the causes of loss of the health certificate may contribute to preventing early retirement. Interventions to prevent these disorders should be evaluated by controlled intervention studies.

  17. Arctic and offshore research. Technology status report

    SciTech Connect

    Not Available

    1985-10-01

    DOE's Morgantown Energy Technology Center (METC) and the DOE Fossil Energy Office of Oil, Gas, and Shale Technology are performing the following activities in Arctic and Offshore Research (AOR): (1) AOR Energy-Related Technology Data Base Development; (2) AOR seminars and workshops; (3) Arctic and Offshore Energy Research Coordination; (4) Arctic and Offshore Research which includes analysis of ice island generation, and prediction of drift paths; field and laboratory determination of (1) the engineering properties of multiyear ice, and (2) the interaction of multiyear ice with offshore structures; analysis of ice gouging in deep water (150 to 210 feet) in the Arctic Ocean, and numerical simulation modeling of the gouging process; analysis of the location and origin of the ice-ridging shear zone; analysis of sea-ice thickness using airborne radar sensing techniques; improvement of permafrost detection techniques, and analysis of permafrost characteristics; investigation of the effects of ice accretion and corrosion on offshore structures; measurements of seismic acceleration and velocity for analyzing vibration in and stability of off-shore structures; detection of oil spills that occur below the Arctic ice pack; analysis of the effects of frost heave and corrosion on pipelines; (5) Advanced Recovery Technologies; and (6) Subice Systems Development. Current activities include determining the Arctic bibliographic data base and initiating most of the research described above (except multiyear ice properties, pipeline research, and subice systems development). 10 refs., 15 figs., 3 tabs.

  18. HIV-1 pre-mRNA commitment to Rev mediated export through PSF and Matrin 3

    SciTech Connect

    Kula, Anna; Gharu, Lavina; Marcello, Alessandro

    2013-01-20

    Human immunodeficiency virus gene expression and replication are regulated at several levels. Incompletely spliced viral RNAs and full-length genomic RNA contain the RRE element and are bound by the viral trans-acting protein Rev to be transported out of the nucleus. Previously we found that the nuclear matrix protein MATR3 was a cofactor of Rev-mediated RNA export. Here we show that the pleiotropic protein PSF binds viral RNA and is associated with MATR3. PSF is involved in the maintenance of a pool of RNA available for Rev activity. However, while Rev and PSF bind the viral pre-mRNA at the site of viral transcription, MATR3 interacts at a subsequent step. We propose that PSF and MATR3 define a novel pathway for RRE-containing HIV-1 RNAs that is hijacked by the viral Rev protein.

  19. Impact of circadian nuclear receptor REV-ERBα on midbrain dopamine production and mood regulation.

    PubMed

    Chung, Sooyoung; Lee, Eun Jeong; Yun, Seongsik; Choe, Han Kyoung; Park, Seong-Beom; Son, Hyo Jin; Kim, Kwang-Soo; Dluzen, Dean E; Lee, Inah; Hwang, Onyou; Son, Gi Hoon; Kim, Kyungjin

    2014-05-08

    The circadian nature of mood and its dysfunction in affective disorders is well recognized, but the underlying molecular mechanisms are still unclear. Here, we show that the circadian nuclear receptor REV-ERBα, which is associated with bipolar disorder, impacts midbrain dopamine production and mood-related behavior in mice. Genetic deletion of the Rev-erbα gene or pharmacological inhibition of REV-ERBα activity in the ventral midbrain induced mania-like behavior in association with a central hyperdopaminergic state. Also, REV-ERBα repressed tyrosine hydroxylase (TH) gene transcription via competition with nuclear receptor-related 1 protein (NURR1), another nuclear receptor crucial for dopaminergic neuronal function, thereby driving circadian TH expression through a target-dependent antagonistic mechanism. In conclusion, we identified a molecular connection between the circadian timing system and mood regulation, suggesting that REV-ERBα could be targeting in the treatment of circadian rhythm-related affective disorders.

  20. Nuclear RNA Export and Packaging Functions of HIV-1 Rev Revisited▿

    PubMed Central

    Blissenbach, Maik; Grewe, Bastian; Hoffmann, Bianca; Brandt, Sabine; Überla, Klaus

    2010-01-01

    Although the viral Rev protein is necessary for HIV replication, its main function in the viral replication cycle has been controversial. Reinvestigating the effect of Rev on the HIV-1 RNA distribution in various cell lines and primary cells revealed that Rev enhanced cytoplasmic levels of the unspliced HIV-1 RNA, mostly 3- to 12-fold, while encapsidation of the RNA and viral infectivity could be stimulated >1,000-fold. Although this clearly questions the general notion that the nuclear export of viral RNAs is the major function of Rev, mechanistically encapsidation seems to be linked to nuclear export, since the tethering of the nuclear export factor TAP to the HIV-1 RNA also enhanced encapsidation. Interference with the formation of an inhibitory ribonucleoprotein complex in the nucleus could lead to enhanced accessibility of the cytoplasmic HIV-1 RNA for translation and encapsidation. This might explain why Rev and tethered TAP exert the same pattern of pleiotropic effects. PMID:20427541

  1. Sky input horn for a far-infrared interferometer

    NASA Technical Reports Server (NTRS)

    Miller, M. S.; Eichhorn, W. L.; Mather, J. C.

    1982-01-01

    A unique design has been developed whereby a compound parabolic concentrator (CPC) and a compound elliptical concentrator (CEC) are joined at their throats. The CPC serves as the field-defining optics, in that it accepts up to a certain maximum acceptance angle and then concentrates this accepted energy at its throat. Energy incident from angles greater than the acceptance angle is rejected. The CEC takes the energy concentrated at the CPC throat and then redirects this energy into a finite-sized pupil a given distance away. The considered design will be used as the sky input horn for a cryogenic far-infrared polarizing interferometer to be flown on NASA's Cosmic Background Explorer satellite. The interferometer will operate at 2 K and measure the 3-K cosmic background radiation of the universe in the 100-micrometer-1-cm spectral range.

  2. A curious pellet from a great horned owl (Bubo virginianus)

    USGS Publications Warehouse

    Woodman, N.; Dove, C.J.; Peurach, S.C.

    2005-01-01

    One of the traditional methods of determining the dietary preferences of owls relies upon the identification of bony remains of prey contained in regurgitated pellets. Discovery of a pellet containing a large, complete primary feather from an adult, male Ring-necked Pheasant (Phasianus colchicus) prompted us to examine in detail a small sample of pellets from a Great Horned Owl (Bubo virginianus). Our analyses of feather and hair remains in these pellets documented the presence of three species of birds and two species of mammals, whereas bones in the pellets represented only mammals. This finding indicates an important bias that challenges the reliability of owl pellet studies making use of only osteological remains.

  3. Tongue adhesion in the horned frog Ceratophrys sp.

    NASA Astrophysics Data System (ADS)

    Kleinteich, Thomas; Gorb, Stanislav N.

    2014-06-01

    Frogs are well-known to capture elusive prey with their protrusible and adhesive tongues. However, the adhesive performance of frog tongues and the mechanism of the contact formation with the prey item remain unknown. Here we measured for the first time adhesive forces and tongue contact areas in living individuals of a horned frog (Ceratophrys sp.) against glass. We found that Ceratophrys sp. generates adhesive forces well beyond its own body weight. Surprisingly, we found that the tongues adhered stronger in feeding trials in which the coverage of the tongue contact area with mucus was relatively low. Thus, besides the presence of mucus, other features of the frog tongue (surface profile, material properties) are important to generate sufficient adhesive forces. Overall, the experimental data shows that frog tongues can be best compared to pressure sensitive adhesives (PSAs) that are of common technical use as adhesive tapes or labels.

  4. Micromachined ultrasonic droplet generator based on a liquid horn structure

    NASA Astrophysics Data System (ADS)

    Meacham, J. M.; Ejimofor, C.; Kumar, S.; Degertekin, F. L.; Fedorov, A. G.

    2004-05-01

    A micromachined ultrasonic droplet generator is developed and demonstrated for drop-on-demand fluid atomization. The droplet generator comprises a bulk ceramic piezoelectric transducer for ultrasound generation, a reservoir for the ejection fluid, and a silicon micromachined liquid horn structure as the nozzle. The nozzles are formed using a simple batch microfabrication process that involves wet etching of (100) silicon in potassium hydroxide solution. Device operation is demonstrated by droplet ejection of water through 30 μm orifices at 1.49 and 2.30 MHz. The finite-element simulations of the acoustic fields in the cavity and electrical impedance of the device are in agreement with the measurements and indicate that the device utilizes cavity resonances in the 1-5 MHz range in conjunction with acoustic wave focusing by the pyramidally shaped nozzles to achieve low power operation.

  5. Tongue adhesion in the horned frog Ceratophrys sp.

    PubMed

    Kleinteich, Thomas; Gorb, Stanislav N

    2014-06-12

    Frogs are well-known to capture elusive prey with their protrusible and adhesive tongues. However, the adhesive performance of frog tongues and the mechanism of the contact formation with the prey item remain unknown. Here we measured for the first time adhesive forces and tongue contact areas in living individuals of a horned frog (Ceratophrys sp.) against glass. We found that Ceratophrys sp. generates adhesive forces well beyond its own body weight. Surprisingly, we found that the tongues adhered stronger in feeding trials in which the coverage of the tongue contact area with mucus was relatively low. Thus, besides the presence of mucus, other features of the frog tongue (surface profile, material properties) are important to generate sufficient adhesive forces. Overall, the experimental data shows that frog tongues can be best compared to pressure sensitive adhesives (PSAs) that are of common technical use as adhesive tapes or labels.

  6. Tongue adhesion in the horned frog Ceratophrys sp.

    PubMed Central

    Kleinteich, Thomas; Gorb, Stanislav N.

    2014-01-01

    Frogs are well-known to capture elusive prey with their protrusible and adhesive tongues. However, the adhesive performance of frog tongues and the mechanism of the contact formation with the prey item remain unknown. Here we measured for the first time adhesive forces and tongue contact areas in living individuals of a horned frog (Ceratophrys sp.) against glass. We found that Ceratophrys sp. generates adhesive forces well beyond its own body weight. Surprisingly, we found that the tongues adhered stronger in feeding trials in which the coverage of the tongue contact area with mucus was relatively low. Thus, besides the presence of mucus, other features of the frog tongue (surface profile, material properties) are important to generate sufficient adhesive forces. Overall, the experimental data shows that frog tongues can be best compared to pressure sensitive adhesives (PSAs) that are of common technical use as adhesive tapes or labels. PMID:24921415

  7. An Anonymous Surveying Protocol via Greenberger-Horne-Zeilinger States

    NASA Astrophysics Data System (ADS)

    Naseri, Mosayeb; Gong, Li-Hua; Houshmand, Monireh; Matin, Laleh Farhang

    2016-10-01

    A new experimentally feasible anonymous survey protocol with authentication using Greenberger-Horne-Zeilinger (GHZ) entangled states is proposed. In this protocol, a chief executive officer (CEO) of a firm or company is trying to find out the effect of a possible action. In order to prepare a fair voting, the CEO would like to make an anonymous survey and is also interested in the total action for the whole company and he doesn't want to have a partial estimate for each department. In our proposal, there are two voters, Alice and Bob, voting on a question with a response of either "yes" or "no" and a tallyman, whose responsibility is to determine whether they have cast the same vote or not. In the proposed protocol the total response of the voters is calculated without revealing the actual votes of the voters.

  8. Presumptive electric cataracts in a Great Horned owl (Bubo virginianus).

    PubMed

    Dees, D Dustin; MacLaren, Nicole E

    2013-01-01

    This case report describes suspected electrocution in a juvenile female Great Horned owl (Bubo virginianus) with subsequent bilateral cataract formation. The bird flew into a high-voltage power line and was immediately rescued. Burn wounds of the head and ataxia with apparent blindness were noted. Initial ophthalmic examination 5 days after the incident revealed bilaterally symmetrical anterior subcapsular vacuolar cataracts with absence of intraocular inflammation and a predominantly clear view to the normal appearing fundus. The bird appeared to be nonvisual. No ophthalmic medications were prescribed at initial examination. Subsequent recheck examination at 8 weeks revealed moderate resolution of the cataracts and improved vision. To the authors' knowledge, this is the first published report of suspected electric cataracts in an avian species. © 2012 American College of Veterinary Ophthalmologists.

  9. Pulmonary carcinoma in a great horned owl (Bubo virginianus).

    PubMed

    Rettenmund, Christy; Sladky, Kurt K; Rodriguez, Daniel; Petersen, Michael; Pinkerton, Marie E; Rao, Deepa

    2010-03-01

    Pulmonary carcinoma was diagnosed in an 18+-year-old captive female great horned owl (Bubo virginianus). The owl presented with a history of progressive weakness and sudden onset of frank blood in the droppings. On physical examination, the owl had multiple white to yellow plaques in the oral cavity, decreased air sac sounds on the right side, dyspnea (during manual restraint), and reduced pectoral musculature. Whole-body radiographs revealed obliteration of the right-sided air sacs, a soft tissue plaque/density in the left caudal thoracic air sac, soft tissue opacity over the coelomic organs, and increased medullary opacity in the distal right humerus. The owl died during anesthetic recovery, and the body was submitted for necropsy. Although the clinical signs, physical examination results, radiographic signs, and gross pathology supported a diagnosis of mycotic infection, such as aspergillosis, histopathology confirmed pulmonary carcinoma with metastases to the air sacs and humerus.

  10. Measurements of very low-sidelobe conical horn antennas

    NASA Technical Reports Server (NTRS)

    Toral, Marco A.; Ratliff, Roger B.; Lecha, Maria C.; Maruschak, John G.; Bennett, Charles L.

    1989-01-01

    A description is given of conical corrugated-horn antennas that were designed for millimeter-wave radiometers with a 7 degree field of view, namely the Differential Microwave Radiometers (DMRs) that will measure the large-angular-scale anisotropy of the cosmic background radiation that is generally thought to be the remnant of the primeval explosion, the Big Bang. The DMRs will be part of the Cosmic Background Explorer (COBE) satellite. Measured test results for three radiometers at 31.4, 53, and 90 GHz are reported along with those of a circular polarization orthomode transducer designed and characterized at 31.4 GHz. The measurement techniques and facilities are described, including an outdoor far-field facility where measurements down to levels 90 dB below the main beam maximum were achieved. The goal of achieving very low-sidelobe antennas with good symmetry has been demonstrated.

  11. Conventional reanastomosis versus laser welding of rat uterine horns.

    PubMed

    Shapiro, A G; Carter, M; Ahmed, A; Sielszak, M W

    1987-04-01

    In this study we compared conventional surgical techniques with those of low-power CO2 lasers (output 140 mW; spot size 0.4 mm) used to weld transected rat uterine horns. On one side a microanastomosis was made by standard surgical technique of 8-0 nylon; the other side was "welded" either after doing the anastomosis with 8-0 sutures or without any anastomotic sutures. Histologic sections obtained from rats' uteri treated with conventional and laser surgery showed that on the laser-treated sutured side there was less necrosis and inflammatory and giant cells. The animals that underwent laser welding without suturing had no necrosis, suppuration, or granulation; giant cells were not present. We conclude that in the tissue from the laser-treated animals, when compared with conventional and laser-with-suture surgery, histologic features indicate healing process by primary intention via an aseptic noninflammatory reaction.

  12. Redefining ecological ethics: science, policy, and philosophy at Cape Horn.

    PubMed

    Frodeman, Robert

    2008-12-01

    In the twentieth century, philosophy (especially within the United States) embraced the notion of disciplinary expertise: philosophical research consists of working with and writing for other philosophers. Projects that involve non-philosophers earn the deprecating title of "applied" philosophy. The University of North Texas (UNT) doctoral program in philosophy exemplifies the possibility of a new model for philosophy, where graduate students are trained in academic philosophy and in how to work with scientists, engineers, and policy makers. This "field" (rather than "applied") approach emphasizes the inter- and transdisciplinary nature of the philosophical enterprise where theory and practice dialectically inform one another. UNT's field station in philosophy at Cape Horn, Patagonia, Chile is one site for developing this ongoing experiment in the theory and practice of interdisciplinary philosophic research and education.

  13. Purifying Greenberger-Horne-Zeilinger states using degenerate quantum codes

    NASA Astrophysics Data System (ADS)

    Ho, K. H.; Chau, H. F.

    2008-10-01

    Degenerate quantum codes are codes that do not reveal the complete error syndrome. Their ability to conceal the complete error syndrome makes them powerful resources in certain quantum-information processing tasks. In particular, the most error-tolerant way to purify depolarized Bell states using one-way communication known to date involves degenerate quantum codes. Here we study three closely related purification schemes for depolarized Greenberger-Horne-Zeilinger states shared among m⩾3 players by means of degenerate quantum codes and one-way classical communications. We find that our schemes tolerate more noise than all other one-way schemes known to date, further demonstrating the effectiveness of degenerate quantum codes in quantum-information processing.

  14. Dogwood borer (Lepidoptera: Sesiidae) infestation of horned oak galls.

    PubMed

    Eliason, E A; Potter, D A

    2000-06-01

    Pin oak, Quercus palustris Muenchhausen, is the primary host for the gall wasp Callirhytis cornigera (Osten Sacken). Woody stem galls formed by C. cornigera may be infested by the dogwood borer, Synanthedon scitula (Harris), an important pest of flowering dogwood, Cornus florida L. Previous research has shown that S. scitula has a bimodal seasonal flight pattern, with peaks in late spring and midsummer. We tested the hypothesis that moths emerging from dogwoods largely account for the first flight pulse, whereas emergence from stem galls contributes disproportionately to the second pulse. Seasonal flight activity of S. scitula was monitored with pheromone traps baited with Z,Z-3,13-octadecadien-1-ol acetate. Traps were hung near plantings of dogwoods in suburban landscapes or near heavily galled pin oaks. Borer emergence from dogwood was monitored by sampling infested trees for pupal exuviae, and from galls that were collected and held in outdoor rearing cages. The impact of S. scitula on C. cornigera larvae was assessed by weighing, measuring, and dissecting galls. Flight activity of S. scitula began on 5 May and ended on 13 October 1999, with peaks in late May and in late July to early August. The flight pattern was similar for the two types of trapping sites, and moths emerged from both hosts during both flight periods. Proportionately more moths emerged from dogwoods during the first flight pulse than during the second, but emergence from galls was nearly evenly divided between the two flight peaks. We therefore reject the hypothesis that emergence of borers from galls contributes disproportionately to the second flight period. Approximately 12-15% of stem galls (2-3 yr old) contained S. scitula larvae. Feeding and tunneling by borers contributed to gall desiccation and reduced horn development, but rarely killed C. cornigera larvae. This study has implications for management of S. scitula because borers emerging from horned oak galls may represent a threat to

  15. Erosional history of Big Horn basin: Mackin revisited

    SciTech Connect

    Palmquist, R.C.

    1983-08-01

    The classic study of the erosional history of Big Horn basin is by Mackin in 1937. In it he studied the terrace levels which ranged in age from Late Tertiary to late Pleistocene. He postulated that the terraces were the product of stream captures or intervals of interglacial stability alternating with glacial incision. More recent studies have revised Mackin's classically simple model. Detailed studies have increased the number of terrace levels, changed the timing of their stability episode, and estimated their ages. The number of terrace levels has been increased to nine along the Greybull and Bighorn Rivers and to six along the Shoshoni River. Because some of the different levels occur along each river, the number of unique levels within the basin is 12. The occurrence of a 600,000 and a 100,000 year old ash on two terrace levels allows the ages of the terraces to be estimated. The estimated ages range from 3 m.y. for the Tatman to 49,000 years for the Himes, which is the lowest level along the Bighorn River. Both ashes were deposited during river stability intervals and indicate that the Bighorn River and its eastern tributaries were stable late in the interglacial episodes. In contrast, the glaciofluvial gravels along the Shoshoni River at Cody indicate a late glacial stability episode for the western tributaries. The terrace cycles along the Bighorn River and its western tributaries are therefore out-of-phase. Comparison of the estimated terrace ages to termination in the marine isotopic record indicates that not all of the Pleistocene climatic cycles are preserved in the Big Horn basin terrace chronology.

  16. Caffeine Abolishes the Ultraviolet-Induced REV3 Translesion Replication Pathway in Mouse Cells

    PubMed Central

    Takezawa, Jun; Aiba, Naomi; Kajiwara, Kagemasa; Yamada, Kouichi

    2011-01-01

    When a replicative DNA polymerase stalls upon encountering a photoproduct on the template strand, it is relieved by other low-processivity polymerase(s), which insert nucleotide(s) opposite the lesion. Using an alkaline sucrose density gradient sedimentation technique, we previously classified this process termed UV-induced translesion replication (UV-TLS) into two types. In human cancer cells or xeroderma pigmentosum variant (XP-V) cells, UV-TLS was inhibited by caffeine or proteasome inhibitors. However, in normal human cells, the process was insensitive to these reagents. Reportedly, in yeast or mammalian cells, REV3 protein (a catalytic subunit of DNA polymerase ζ) is predominantly involved in the former type of TLS. Here, we studied UV-TLS in fibroblasts derived from the Rev3-knockout mouse embryo (Rev3KO-MEF). In the wild-type MEF, UV-TLS was slow (similar to that of human cancer cells or XP-V cells), and was abolished by caffeine or MG-262. In 2 cell lines of Rev3KO-MEF (Rev3−/− p53−/−), UV-TLS was not observed. In p53KO-MEF, which is a strict control for Rev3KO-MEF, the UV-TLS response was similar to that of the wild-type. Introduction of the Rev3 expression plasmid into Rev3KO-MEF restored the UV-TLS response in selected stable transformants. In some transformants, viability to UV was the same as that in the wild-type, and the death rate was increased by caffeine. Our findings indicate that REV3 is predominantly involved in UV-TLS in mouse cells, and that the REV3 translesion pathway is suppressed by caffeine or proteasome inhibitors. PMID:22272088

  17. RevBayes: Bayesian Phylogenetic Inference Using Graphical Models and an Interactive Model-Specification Language

    PubMed Central

    Höhna, Sebastian; Landis, Michael J.

    2016-01-01

    Programs for Bayesian inference of phylogeny currently implement a unique and fixed suite of models. Consequently, users of these software packages are simultaneously forced to use a number of programs for a given study, while also lacking the freedom to explore models that have not been implemented by the developers of those programs. We developed a new open-source software package, RevBayes, to address these problems. RevBayes is entirely based on probabilistic graphical models, a powerful generic framework for specifying and analyzing statistical models. Phylogenetic-graphical models can be specified interactively in RevBayes, piece by piece, using a new succinct and intuitive language called Rev. Rev is similar to the R language and the BUGS model-specification language, and should be easy to learn for most users. The strength of RevBayes is the simplicity with which one can design, specify, and implement new and complex models. Fortunately, this tremendous flexibility does not come at the cost of slower computation; as we demonstrate, RevBayes outperforms competing software for several standard analyses. Compared with other programs, RevBayes has fewer black-box elements. Users need to explicitly specify each part of the model and analysis. Although this explicitness may initially be unfamiliar, we are convinced that this transparency will improve understanding of phylogenetic models in our field. Moreover, it will motivate the search for improvements to existing methods by brazenly exposing the model choices that we make to critical scrutiny. RevBayes is freely available at http://www.RevBayes.com. [Bayesian inference; Graphical models; MCMC; statistical phylogenetics.] PMID:27235697

  18. The orphan receptor Rev-erbα gene is a target of the circadian clock pacemaker

    PubMed Central

    Triqueneaux, Gérard; Thenot, Sandrine; Kakizawa, Tomoko; Antoch, Marina P; Safi, Rachid; Takahashi, Joseph S; Delaunay, Franck; Laudet, Vincent

    2013-01-01

    Rev-erbα is a ubiquitously expressed orphan nuclear receptor which functions as a constitutive transcriptional repressor and is expressed in vertebrates according to a robust circadian rhythm. We report here that two Rev-erbα mRNA isoforms, namely Rev-erbα1 and Rev-erbα2, are generated through alternative promoter usage and that both show a circadian expression pattern in an in vitro system using serum-shocked fibroblasts. Both promoter regions P1 (Rev-erbα1) and P2 (Rev-erbα2) contain several E-box DNA sequences, which function as response elements for the core circadian-clock components: CLOCK and BMAL1. The CLOCK–BMAL1 heterodimer stimulates the activity of both P1 and P2 promoters in transient transfection assay by 3–6-fold. This activation was inhibited by the overexpression of CRY1, a component of the negative limb of the circadian transcriptional loop. Critical E-box elements were mapped within both promoters. This regulation is conserved in vertebrates since we found that the CLOCK–BMAL1 heterodimer also regulates the zebrafish Rev-erbα gene. In line with these data Rev-erbα circadian expression was strongly impaired in the livers of Clock mutant mice and in the pineal glands of zebrafish embryos treated with Clock and Bmal1 antisense oligonucleotides. Together these data demonstrate that CLOCK is a critical regulator of Rev-erbα circadian gene expression in evolutionarily distant vertebrates and suggest a role for Rev-erbα in the circadian clock output. PMID:15591021

  19. Medical evacuations from offshore structures.

    PubMed Central

    Norman, J N; Ballantine, B N; Brebner, J A; Brown, B; Gauld, S J; Mawdsley, J; Roythorne, C; Valentine, M J; Wilcock, S E

    1988-01-01

    A retrospective study was carried out on medical evacuations from the installations of four major oil or gas producing companies, or both, operating offshore on the United Kingdom continental shelf. The study covered 1976-84 during which 2162 evacuations were recorded. Of these, 137 (7.7%) required the use of a chartered helicopter. In the earlier years of the study there were substantially more injuries sustained than episodes of illness recorded but from 1980 onwards the cases of illness equalled those of injury. Using the International Classification of Diseases, the digestive system was responsible for most evacuations for illness and of those, about half (115 evacuations) were for dental problems. Suspected fractures were responsible for about one third of those evacuated for an injury but injuries of hands and eye conditions were particularly common, accounting for 25% of all evacuations. As the age of the evacuee increased the proportion of evacuations for injury decreased and that for illness increased. The mean age for evacuation for injury was 28.3 years and for illness 34.4 years. Few evacuations were required for those aged over 45. PMID:3179237

  20. Medical evacuations from offshore structures.

    PubMed

    Norman, J N; Ballantine, B N; Brebner, J A; Brown, B; Gauld, S J; Mawdsley, J; Roythorne, C; Valentine, M J; Wilcock, S E

    1988-09-01

    A retrospective study was carried out on medical evacuations from the installations of four major oil or gas producing companies, or both, operating offshore on the United Kingdom continental shelf. The study covered 1976-84 during which 2162 evacuations were recorded. Of these, 137 (7.7%) required the use of a chartered helicopter. In the earlier years of the study there were substantially more injuries sustained than episodes of illness recorded but from 1980 onwards the cases of illness equalled those of injury. Using the International Classification of Diseases, the digestive system was responsible for most evacuations for illness and of those, about half (115 evacuations) were for dental problems. Suspected fractures were responsible for about one third of those evacuated for an injury but injuries of hands and eye conditions were particularly common, accounting for 25% of all evacuations. As the age of the evacuee increased the proportion of evacuations for injury decreased and that for illness increased. The mean age for evacuation for injury was 28.3 years and for illness 34.4 years. Few evacuations were required for those aged over 45.

  1. Norwegian Offshore Stratigraphic Lexicon (NORLEX)

    NASA Astrophysics Data System (ADS)

    Gradstein, Felix M.; Hammer, Oyvind; Brunstad, Harald; Charnock, Mike; Hellem, Terje; Sigve Lervik, Kjell; Anthonissen, Erik

    2010-05-01

    The Norwegian Offshore Stratigraphic Lexicon (NORLEX) provides a relational stratigraphic database for the North Sea, Norwegian Sea, Barents Sea and Svalbard. Both regional lithostratigraphy and biostratigraphy are being substantially updated, following guidelines laid out in the International Stratigraphic Guide. The main body of information developed is available as a petroleum consortium (oracle-style) database, and the new lithostratigraphic definitions as a public domain (paper) document. NORLEX is presented as a browsing website via the internet at http://www.nhm.uio.no/norlex. Seismic cross-sections, core photographs, well logs, field outcrops, microfossil occurrences and other vital attributes are relationally cross-linked. In addition, there are menus for instantly finding updated formation and member tops or microfossil events in all wells, plus a map contouring routine for unit thicknesses and depths. Several new initiatives will expand data and user coverage: 1. Overhaul of Mesozoic stratigraphy, especially Triassic and Cretaceous, in the Barents Sea. 2. Coverage of East Greenland 3. Linkage to UK and Belgium and The Netherlands surface and subsurface stratigraphy 4. Creation of a Sequence Stratigraphic Framework for specific regions. 5. A national microfossil atlas to support zonations 6. Tight linkage to the basin datapacks in TimeScaleCreator Pro, as developed for Australia, New Zealand, Brasil, Gulf of Mexico, Canada and Russia. NORLEX may thus evolve to become STRATLEX, covering many basin regions.

  2. Offshore oil and the coastline

    NASA Astrophysics Data System (ADS)

    Bell, Peter M.

    A radical, accelerated 5-year plan to offer 875 million acres (of which 20 million could actually be leased for oil and gas extraction purposes) on the outer continental shelf (OCS) could result in the release of large volumes of drilling wastes and spillage (Environ. Sci. Tech., Nov. 1981). The actual leasing, under the 5-year plan proposed by Secretary of the Interior James G. Watt, could amount to 4-5 million acres per year—about 10 times as much, on the average, as had been leased over the past 25 years. Regulations on the environmental effects may be less complicated yet more effective in that impact statements will cover large areas instead of the tract-by-tract statements now required. A number of the new offshore leasing areas, for example, the Alaska Coast (Cook Inlet, Beaufort Bay, Gulf of Alaska), the Blake Plateau and Baltimore Canyon, and the Georges Bank, are extremely valuable in terms of renewable resources and potentially fragile in terms of environmental conditions. Fishing interests in these areas have produced considerable controversy over the planned sale of petroleum rights.

  3. Problems unique to offshore measurement

    SciTech Connect

    Young, J.G.

    1995-12-01

    Most of us have arrived at this meeting in some kind of company provided transportation. This is supplied in order for us to do our assigned jobs. These may be trucks, or cars, or even helicopters, and maybe boats. All who are involved in the offshore industry know that transportation is the cost costly of all. The helicopter bill for our company is a bill which costs us somewhere in the 9 million dollar range. Since this costs us so much, we are constantly looking at ways to reduce this. The helicopters we use cost us $510.00 a flying hour plus $28,000.00 a month for lease. These helicopters fly in the neighborhood of 140 miles per hour. You can quickly see how fast the cost can climb. We have two technicians, along with a pilot, and approx. 400 lbs of test gear and spare parts. You have to carry all you think you will need for the day`s activities because it`s a long and costly trip to go pick up some gasket material or an orifice plate.

  4. Elaborate horns in a giant rhinoceros beetle incur negligible aerodynamic costs.

    PubMed

    McCullough, Erin L; Tobalske, Bret W

    2013-05-07

    Sexually selected ornaments and weapons are among nature's most extravagant morphologies. Both ornaments and weapons improve a male's reproductive success; yet, unlike ornaments that need only attract females, weapons must be robust and functional structures because they are frequently tested during male-male combat. Consequently, weapons are expected to be particularly costly to bear. Here, we tested the aerodynamic costs of horns in the giant rhinoceros beetle, Trypoxylus dichotomus. We predicted that the long, forked head horn would have three main effects on flight performance: increased body mass, an anterior shift in the centre of mass and increased body drag. We found that the horns were surprisingly lightweight, and therefore had a trivial effect on the male beetles' total mass and mass distribution. Furthermore, because beetles typically fly at slow speeds and high body angles, horns had little effect on total body drag. Together, the weight and the drag of horns increased the overall force required to fly by less than 3 per cent, even in the largest males. Because low-cost structures are expected to be highly evolutionarily labile, the fact that horns incur very minor flight costs may have permitted both the elaboration and diversification of rhinoceros beetle horns.

  5. Elaborate horns in a giant rhinoceros beetle incur negligible aerodynamic costs

    PubMed Central

    McCullough, Erin L.; Tobalske, Bret W.

    2013-01-01

    Sexually selected ornaments and weapons are among nature's most extravagant morphologies. Both ornaments and weapons improve a male's reproductive success; yet, unlike ornaments that need only attract females, weapons must be robust and functional structures because they are frequently tested during male–male combat. Consequently, weapons are expected to be particularly costly to bear. Here, we tested the aerodynamic costs of horns in the giant rhinoceros beetle, Trypoxylus dichotomus. We predicted that the long, forked head horn would have three main effects on flight performance: increased body mass, an anterior shift in the centre of mass and increased body drag. We found that the horns were surprisingly lightweight, and therefore had a trivial effect on the male beetles' total mass and mass distribution. Furthermore, because beetles typically fly at slow speeds and high body angles, horns had little effect on total body drag. Together, the weight and the drag of horns increased the overall force required to fly by less than 3 per cent, even in the largest males. Because low-cost structures are expected to be highly evolutionarily labile, the fact that horns incur very minor flight costs may have permitted both the elaboration and diversification of rhinoceros beetle horns. PMID:23486444

  6. Novel Insights into the Bovine Polled Phenotype and Horn Ontogenesis in Bovidae

    PubMed Central

    Allais-Bonnet, Aurélie; Grohs, Cécile; Medugorac, Ivica; Krebs, Stefan; Djari, Anis; Graf, Alexander; Fritz, Sébastien; Seichter, Doris; Baur, Aurélia; Russ, Ingolf; Bouet, Stéphan; Rothammer, Sophie; Wahlberg, Per; Esquerré, Diane; Hoze, Chris; Boussaha, Mekki; Weiss, Bernard; Thépot, Dominique; Fouilloux, Marie-Noëlle; Rossignol, Marie-Noëlle; van Marle-Köster, Este; Hreiðarsdóttir, Gunnfríður Elín; Barbey, Sarah; Dozias, Dominique; Cobo, Emilie; Reversé, Patrick; Catros, Olivier; Marchand, Jean-Luc; Soulas, Pascal; Roy, Pierre; Marquant-Leguienne, Brigitte; Le Bourhis, Daniel; Clément, Laetitia; Salas-Cortes, Laura; Venot, Eric; Pannetier, Maëlle; Phocas, Florence; Klopp, Christophe; Rocha, Dominique; Fouchet, Michel; Journaux, Laurent; Bernard-Capel, Carine; Ponsart, Claire; Eggen, André; Blum, Helmut; Gallard, Yves; Boichard, Didier; Pailhoux, Eric; Capitan, Aurélien

    2013-01-01

    Despite massive research efforts, the molecular etiology of bovine polledness and the developmental pathways involved in horn ontogenesis are still poorly understood. In a recent article, we provided evidence for the existence of at least two different alleles at the Polled locus and identified candidate mutations for each of them. None of these mutations was located in known coding or regulatory regions, thus adding to the complexity of understanding the molecular basis of polledness. We confirm previous results here and exhaustively identify the causative mutation for the Celtic allele (PC) and four candidate mutations for the Friesian allele (PF). We describe a previously unreported eyelash-and-eyelid phenotype associated with regular polledness, and present unique histological and gene expression data on bovine horn bud differentiation in fetuses affected by three different horn defect syndromes, as well as in wild-type controls. We propose the ectopic expression of a lincRNA in PC/p horn buds as a probable cause of horn bud agenesis. In addition, we provide evidence for an involvement of OLIG2, FOXL2 and RXFP2 in horn bud differentiation, and draw a first link between bovine, ovine and caprine Polled loci. Our results represent a first and important step in understanding the genetic pathways and key process involved in horn bud differentiation in Bovidae. PMID:23717440

  7. Analysis of soft and hard strip-loaded horns using a circular cylindrical model

    NASA Astrophysics Data System (ADS)

    Lier, Erik

    1990-06-01

    Strip-loaded horns with transverse (soft) and longitudinal (hard) strips are analyzed theoretically. The method is based on a circular cylindrical and uniform waveguide model with a periodic strip structure. The field is represented by an infinite series of space harmonics (Floquet modes) in the air-filled central region and in the dielectrically filled wall region. The tangential field is forced to be continuous across the air-dielectric boundary. The propagation constant and the total field (including the hybrid factor) can be determined by solving the resulting matrix equations. The convergence of the solution has been accelerated by calculating the higher-order terms analytically. It is shown that the soft-strip-loaded horn in principle exhibits the same electrical behavior as a corrugated horn. The horn represents an interesting alternative to the corrugated horn in wide-band or dual-band applications, in particular for millimeter waves and for lightweight applications onboard satellites. The hard-strip-loaded horn has potentially high gain and low cross polarization over a certain frequency range, dependent on the horn dimensions, thickness of the dielectric wall and on how strongly the stripline modes are being excited.

  8. Crossed receptive field components and crossed dendrites in cat sacrocaudal dorsal horn.

    PubMed

    Gladfelter, W E; Millecchia, R J; Pubols, L M; Sonty, R V; Ritz, L A; Covalt-Dunning, D; Culberson, J; Brown, P B

    1993-10-01

    The hypothesis that sacrocaudal dorsal horn neurons with crossed receptive field components on the tail have dendrites which cross to the contralateral dorsal horn was tested in a combined electrophysiological and morphological study. Dorsal horn cells in the sacrocaudal spinal cord of anesthetized cats were penetrated with horseradish peroxidase-filled microelectrodes. After mapping their low threshold mechanoreceptive fields, cells were iontophoretically injected with horseradish peroxidase. A sample of 16 well-stained cells was obtained in laminae III and IV. Cells with receptive fields crossing the dorsal midline of the tail (n = 8) had somata in the lateral ipsilateral dorsal horn, and some of these cells (5/8) had dendrites which crossed to the lateral contralateral dorsal horn. Cells with receptive fields spanning the ventral midline (n = 2) were located near the center of the fused dorsal horn, and one of these had bilateral dendrites in this region. Cells with receptive fields on the lateral tail, crossing neither the dorsal nor the ventral midline (n = 6), had cell bodies in the middle of the ipsilateral dorsal horn; half had only ipsilateral dendrites, and half had crossed dendritic branches. Although the relationship between cell receptive field (RF) location (RF center, expressed as distance from tips of toes) and mediolateral location of the cell body was statistically significant, the correlation between crossed RF components and crossed dendritic branches was not significant.

  9. Responses of spinal dorsal horn neurons to foot movements in rats with a sprained ankle

    PubMed Central

    Kim, Jae Hyo; Kim, Hee Young; Chung, Kyungsoon

    2011-01-01

    Acute ankle injuries are common problems and often lead to persistent pain. To investigate the underlying mechanism of ankle sprain pain, the response properties of spinal dorsal horn neurons were examined after ankle sprain. Acute ankle sprain was induced manually by overextending the ankle of a rat hindlimb in a direction of plantarflexion and inversion. The weight-bearing ratio (WBR) of the affected foot was used as an indicator of pain. Single unit activities of dorsal horn neurons in response to plantarflexion and inversion of the foot or ankle compression were recorded from the medial part of the deep dorsal horn, laminae IV-VI, in normal and ankle-sprained rats. One day after ankle sprain, rats showed significantly reduced WBRs on the affected foot, and this reduction was partially restored by systemic morphine. The majority of deep dorsal horn neurons responded to a single ankle stimulus modality. After ankle sprain, the mean evoked response rates were significantly increased, and afterdischarges were developed in recorded dorsal horn neurons. The ankle sprain-induced enhanced evoked responses were significantly reduced by morphine, which was reversed by naltrexone. The data indicate that movement-specific dorsal horn neuron responses were enhanced after ankle sprain in a morphine-dependent manner, thus suggesting that hyperactivity of dorsal horn neurons is an underlying mechanism of pain after ankle sprain. PMID:21389306

  10. Differential recruitment of limb patterning genes during development and diversification of beetle horns.

    PubMed

    Moczek, Armin P; Rose, Debra J

    2009-06-02

    The origins of novel complex phenotypes represent one of the most fundamental, yet largely unresolved, issues in evolutionary biology. Here we explore the developmental genetic regulation of beetle horns, a class of traits that lacks obvious homology to traits in other insects. Furthermore, beetle horns are remarkably diverse in their expression, including sexual dimorphisms, male dimorphisms, and interspecific differences in location of horn expression. At the same time, beetle horns share aspects of their development with that of more traditional appendages. We used larval RNA interference-mediated gene function analysis of 3 cardinal insect appendage patterning genes, dachshund, homothorax, and Distal-less, to investigate their role in development and diversification of beetle horns within and between species. Transcript depletion of all 3 patterning genes generated phenotypic effects very similar to those documented in previous studies that focused on general insect development. In addition, we found that Distal-less and homothorax, but not dachshund, regulate horn expression in a species-, sex-, body region-, and body size-dependent manner. Our results demonstrate differential co-option of appendage patterning genes during the evolution and radiation of beetle horns. Furthermore, our results illustrate that regulatory genes whose functions are otherwise highly conserved nevertheless retain the capacity to acquire additional functions, and that little phylogenetic distance appears necessary for the evolution of sex- and species-specific differences in these functions.

  11. The evolution of fossoriality and the adaptive role of horns in the Mylagaulidae (Mammalia: Rodentia)

    PubMed Central

    Hopkins, Samantha S.B

    2005-01-01

    Ceratogaulus, a member of the extinct fossorial rodent clade Mylagaulidae, is the only known rodent with horns and the smallest known horned mammal. The function of the large, dorsally projecting nasal horns on this burrowing animal has been the subject of wide speculation among palaeontologists; suggested uses range from sexual combat to burrowing. Mammals have evolved adaptations for digging repeatedly; horns and other cranial appendages have also evolved numerous times. These two adaptations co-occur in mammals extremely rarely: only two fossil genera (Ceratogaulus and the xenarthran Peltephilus) and no extant mammals are both horned and fossorial. Tracing the evolution of fossoriality in aplodontoid rodents (the larger clade to which Ceratogaulus belongs) reveals that Ceratogaulus descended from ancestors who dug by head-lifting. Whereas this suggests an obvious explanation for the horns of this rodent, evidence from functional morphology, anatomy, phylogeny and geologic context indicates that the horns in Ceratogaulus were used for defence, rather than digging, and evolved to offset increased predation costs associated with spending more time foraging above ground as body size increased. PMID:16087426

  12. Pyrosequencing-Based Analysis of the Microbiome Associated with the Horn Fly, Haematobia irritans

    PubMed Central

    Palavesam, Azhahianambi; Guerrero, Felix D.; Heekin, Andrew M.; Wang, Ju; Dowd, Scot E.; Sun, Yan; Foil, Lane D.; Pérez de León, Adalberto A.

    2012-01-01

    The horn fly, Haematobia irritans, is one of the most economically important pests of cattle. Insecticides have been a major element of horn fly management programs. Growing concerns with insecticide resistance, insecticide residues on farm products, and non-availability of new generation insecticides, are serious issues for the livestock industry. Alternative horn fly control methods offer the promise to decrease the use of insecticides and reduce the amount of insecticide residues on livestock products and give an impetus to the organic livestock farming segment. The horn fly, an obligatory blood feeder, requires the help of microflora to supply additional nutrients and metabolize the blood meal. Recent advancements in DNA sequencing methodologies enable researchers to examine the microflora diversity independent of culture methods. We used the bacterial 16S tag-encoded FLX-titanium amplicon pyrosequencing (bTEFAP) method to carry out the classification analysis of bacterial flora in adult female and male horn flies and horn fly eggs. The bTEFAP method identified 16S rDNA sequences in our samples which allowed the identification of various prokaryotic taxa associated with the life stage examined. This is the first comprehensive report of bacterial flora associated with the horn fly using a culture-independent method. Several rumen, environmental, symbiotic and pathogenic bacteria associated with the horn fly were identified and quantified. This is the first report of the presence of Wolbachia in horn flies of USA origin and is the first report of the presence of Rikenella in an obligatory blood feeding insect. PMID:23028533

  13. RNA helicase MOV10 functions as a co-factor of HIV-1 Rev to facilitate Rev/RRE-dependent nuclear export of viral mRNAs.

    PubMed

    Huang, Feng; Zhang, Junsong; Zhang, Yijun; Geng, Guannan; Liang, Juanran; Li, Yingniang; Chen, Jingliang; Liu, Chao; Zhang, Hui

    2015-12-01

    Human immunodeficiency virus type 1 (HIV-1) exploits multiple host factors during its replication. The REV/RRE-dependent nuclear export of unspliced/partially spliced viral transcripts needs the assistance of host proteins. Recent studies have shown that MOV10 overexpression inhibited HIV-1 replication at various steps. However, the endogenous MOV10 was required in certain step(s) of HIV-1 replication. In this report, we found that MOV10 potently enhances the nuclear export of viral mRNAs and subsequently increases the expression of Gag protein and other late products through affecting the Rev/RRE axis. The co-immunoprecipitation analysis indicated that MOV10 interacts with Rev in an RNA-independent manner. The DEAG-box of MOV10 was required for the enhancement of Rev/RRE-dependent nuclear export and the DEAG-box mutant showed a dominant-negative activity. Our data propose that HIV-1 utilizes the anti-viral factor MOV10 to function as a co-factor of Rev and demonstrate the complicated effects of MOV10 on HIV-1 life cycle. Copyright © 2015 Elsevier Inc. All rights reserved.

  14. Insights into the development and evolution of exaggerated traits using de novo transcriptomes of two species of horned scarab beetles.

    PubMed

    Warren, Ian A; Vera, J Cristobal; Johns, Annika; Zinna, Robert; Marden, James H; Emlen, Douglas J; Dworkin, Ian; Lavine, Laura C

    2014-01-01

    Scarab beetles exhibit an astonishing variety of rigid exo-skeletal outgrowths, known as "horns". These traits are often sexually dimorphic and vary dramatically across species in size, shape, location, and allometry with body size. In many species, the horn exhibits disproportionate growth resulting in an exaggerated allometric relationship with body size, as compared to other traits, such as wings, that grow proportionately with body size. Depending on the species, the smallest males either do not produce a horn at all, or they produce a disproportionately small horn for their body size. While the diversity of horn shapes and their behavioural ecology have been reasonably well studied, we know far less about the proximate mechanisms that regulate horn growth. Thus, using 454 pyrosequencing, we generated transcriptome profiles, during horn growth and development, in two different scarab beetle species: the Asian rhinoceros beetle, Trypoxylus dichotomus, and the dung beetle, Onthophagus nigriventris. We obtained over half a million reads for each species that were assembled into over 6,000 and 16,000 contigs respectively. We combined these data with previously published studies to look for signatures of molecular evolution. We found a small subset of genes with horn-biased expression showing evidence for recent positive selection, as is expected with sexual selection on horn size. We also found evidence of relaxed selection present in genes that demonstrated biased expression between horned and horn-less morphs, consistent with the theory of developmental decoupling of phenotypically plastic traits.

  15. Computational modeling and experimental studies of the dynamic performance of ultrasonic horn profiles used in plastic welding.

    PubMed

    Roopa Rani, M; Rudramoorthy, R

    2013-03-01

    Ultrasonic horns are tuned components designed to vibrate in a longitudinal mode at ultrasonic frequencies. Reliable performance of such horns is normally decided by the uniformity of vibration amplitude at the working surface and the stress developed during loading condition. The horn design engineer must pay particular attention to designing a tool that will produce the desired amplitude without fracturing. The present work discusses horn configurations which satisfy these criteria and investigates the design requirements of horns in ultrasonic system. Different horn profiles for ultrasonic welding of thermoplastics have been characterized in terms of displacement amplitude and von-Mises stresses using modal and harmonic analysis. To validate the simulated results, five different horns are fabricated from Aluminum, tested and tuned to the operating frequency. Standard ABS plastic parts are welded using these horns. Temperature developed during the welding of ABS test parts using different horns is recorded using sensors and National Instruments (NIs) data acquisition system. The recorded values are compared with the predicted values. Experimental results show that welding using a Bezier horn has a high interface temperature and the welded joints had higher strength as compared to the other horn profiles. Copyright © 2012 Elsevier B.V. All rights reserved.

  16. Opiates and Pain Pathways: Demonstration of Enkephalin Synapses on Dorsal Horn Projection Neurons

    NASA Astrophysics Data System (ADS)

    Ruda, M. A.

    1982-03-01

    The participation of the opiate peptide enkephalin in the neural circuitry of the dorsal horn was examined at the light and ultrastructural level through the use of the combined techniques of immunocytochemistry and retrograde transport of horseradish peroxidase. Enkephalin immunoreactive axonal endings made direct synaptic contact with the soma and proximal dendrites of dorsal horn thalamic projection neurons. This observation demonstrates that one major synaptic site of enkephalin modulation of the transfer of nociceptive information in the dorsal horn is on the projection neurons themselves.

  17. Size-specific defensive structures in a horned weevil confirm a classic battle plan: avoid fights with larger opponents.

    PubMed Central

    Eberhard, W G; Garcia, J M; Lobo, J

    2000-01-01

    A rigid sheath that extends deep into the prothorax of male Parisoschoenus expositus serves to receive the horns of opponents in battles over females. The male cannot use his own horn unless he receives his opponent's horn in his sheath. The length of a male's sheath is always approximately equal to the length of his own horns, so his body design is appropriate only for horn-locking battles with males whose horns are equal to or shorter than his own. Horn length and sheath length are dimorphic with respect to each other and to an indicator of body size. The switch points between morphs are very nearly the same for several relationships, so the different aspects of fighting morphology are tightly coordinated. PMID:10885518

  18. 49 CFR 222.33 - Can locomotive horns be silenced at an individual public highway-rail grade crossing which is not...

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 49 Transportation 4 2011-10-01 2011-10-01 false Can locomotive horns be silenced at an individual public highway-rail grade crossing which is not within a quiet zone? 222.33 Section 222.33 Transportation... Locomotive Horn Silenced Horns at Individual Crossings § 222.33 Can locomotive horns be silenced at...

  19. The Nuclear Receptor REV-ERBα Regulates Fabp7 and Modulates Adult Hippocampal Neurogenesis

    PubMed Central

    Schnell, Anna; Chappuis, Sylvie; Schmutz, Isabelle; Brai, Emanuele; Ripperger, Jürgen A.; Schaad, Olivier; Welzl, Hans; Descombes, Patrick; Alberi, Lavinia; Albrecht, Urs

    2014-01-01

    The function of the nuclear receptor Rev-erbα (Nr1d1) in the brain is, apart from its role in the circadian clock mechanism, unknown. Therefore, we compared gene expression profiles in the brain between wild-type and Rev-erbα knock-out (KO) animals. We identified fatty acid binding protein 7 (Fabp7, Blbp) as a direct target of repression by REV-ERBα. Loss of Rev-erbα manifested in memory and mood related behavioral phenotypes and led to overexpression of Fabp7 in various brain areas including the subgranular zone (SGZ) of the hippocampus, where neuronal progenitor cells (NPCs) can initiate adult neurogenesis. We found increased proliferation of hippocampal neurons and loss of its diurnal pattern in Rev-erbα KO mice. In vitro, proliferation and migration of glioblastoma cells were affected by manipulating either Fabp7 expression or REV-ERBα activity. These results suggest an important role of Rev-erbα and Fabp7 in adult neurogenesis, which may open new avenues for treatment of gliomas as well as neurological diseases such as depression and Alzheimer. PMID:24932636

  20. Behavioral changes and dopaminergic dysregulation in mice lacking the nuclear receptor Rev-erbα.

    PubMed

    Jager, Jennifer; O'Brien, W Timothy; Manlove, Jessica; Krizman, Elizabeth N; Fang, Bin; Gerhart-Hines, Zachary; Robinson, Michael B; Klein, Peter S; Lazar, Mitchell A

    2014-04-01

    The regulation of behavior by the molecular components of the circadian clock is not well understood. Here we report that mice lacking the nuclear receptor Rev-erbα, a potent transcriptional repressor and core clock component, displayed marked hyperactivity and impaired response habituation in novel environments. In addition, Rev-erbα knockout (KO) mice were deficient in short-term, long-term, and contextual memories and also showed impairment in nest-building ability. Together, these results suggest that Rev-erbα KO mice manifest defective hippocampal function. Interestingly, the changes in novelty-induced locomotor activity of Rev-erbα KO mice were comparable at multiple times of day, potentially due to the muted amplitude of Rev-erbα oscillation in the hippocampus of wild-type mice. Hippocampal dopamine turnover was increased in Rev-erbα KO mice, due to up-regulation of tyrosine hydroxylase, the rate-limiting enzyme in dopamine production, and pharmacologic inhibition of tyrosine hydroxylase activity partially rescued locomotor hyperactivity. These findings reveal a novel, nonredundant function for Rev-erbα that links a core component of the circadian gene-regulatory network to the control of dopaminergic and hippocampus-dependent behaviors.

  1. Behavioral Changes and Dopaminergic Dysregulation in Mice Lacking the Nuclear Receptor Rev-erbα

    PubMed Central

    Jager, Jennifer; O'Brien, W. Timothy; Manlove, Jessica; Krizman, Elizabeth N.; Fang, Bin; Gerhart-Hines, Zachary; Robinson, Michael B.; Klein, Peter S.

    2014-01-01

    The regulation of behavior by the molecular components of the circadian clock is not well understood. Here we report that mice lacking the nuclear receptor Rev-erbα, a potent transcriptional repressor and core clock component, displayed marked hyperactivity and impaired response habituation in novel environments. In addition, Rev-erbα knockout (KO) mice were deficient in short-term, long-term, and contextual memories and also showed impairment in nest-building ability. Together, these results suggest that Rev-erbα KO mice manifest defective hippocampal function. Interestingly, the changes in novelty-induced locomotor activity of Rev-erbα KO mice were comparable at multiple times of day, potentially due to the muted amplitude of Rev-erbα oscillation in the hippocampus of wild-type mice. Hippocampal dopamine turnover was increased in Rev-erbα KO mice, due to up-regulation of tyrosine hydroxylase, the rate-limiting enzyme in dopamine production, and pharmacologic inhibition of tyrosine hydroxylase activity partially rescued locomotor hyperactivity. These findings reveal a novel, nonredundant function for Rev-erbα that links a core component of the circadian gene-regulatory network to the control of dopaminergic and hippocampus-dependent behaviors. PMID:24552589

  2. The nuclear receptor REV-ERBα regulates Fabp7 and modulates adult hippocampal neurogenesis.

    PubMed

    Schnell, Anna; Chappuis, Sylvie; Schmutz, Isabelle; Brai, Emanuele; Ripperger, Jürgen A; Schaad, Olivier; Welzl, Hans; Descombes, Patrick; Alberi, Lavinia; Albrecht, Urs

    2014-01-01

    The function of the nuclear receptor Rev-erbα (Nr1d1) in the brain is, apart from its role in the circadian clock mechanism, unknown. Therefore, we compared gene expression profiles in the brain between wild-type and Rev-erbα knock-out (KO) animals. We identified fatty acid binding protein 7 (Fabp7, Blbp) as a direct target of repression by REV-ERBα. Loss of Rev-erbα manifested in memory and mood related behavioral phenotypes and led to overexpression of Fabp7 in various brain areas including the subgranular zone (SGZ) of the hippocampus, where neuronal progenitor cells (NPCs) can initiate adult neurogenesis. We found increased proliferation of hippocampal neurons and loss of its diurnal pattern in Rev-erbα KO mice. In vitro, proliferation and migration of glioblastoma cells were affected by manipulating either Fabp7 expression or REV-ERBα activity. These results suggest an important role of Rev-erbα and Fabp7 in adult neurogenesis, which may open new avenues for treatment of gliomas as well as neurological diseases such as depression and Alzheimer.

  3. Rev1 is a base excision repair enzyme with 5′-deoxyribose phosphate lyase activity

    PubMed Central

    Prasad, Rajendra; Poltoratsky, Vladimir; Hou, Esther W.; Wilson, Samuel H.

    2016-01-01

    Rev1 is a member of the Y-family of DNA polymerases and is known for its deoxycytidyl transferase activity that incorporates dCMP into DNA and its ability to function as a scaffold factor for other Y-family polymerases in translesion bypass events. Rev1 also is involved in mutagenic processes during somatic hypermutation of immunoglobulin genes. In light of the mutation pattern consistent with dCMP insertion observed earlier in mouse fibroblast cells treated with a base excision repair-inducing agent, we questioned whether Rev1 could also be involved in base excision repair (BER). Here, we uncovered a weak 5′-deoxyribose phosphate (5′-dRP) lyase activity in mouse Rev1 and demonstrated the enzyme can mediate BER in vitro. The full-length Rev1 protein and its catalytic core domain are similar in their ability to support BER in vitro. The dRP lyase activity in both of these proteins was confirmed by NaBH4 reduction of the Schiff base intermediate and kinetics studies. Limited proteolysis, mass spectrometry and deletion analysis localized the dRP lyase active site to the C-terminal segment of Rev1's catalytic core domain. These results suggest that Rev1 could serve as a backup polymerase in BER and could potentially contribute to AID-initiated antibody diversification through this activity. PMID:27683219

  4. Rev1, Rev3, or Rev7 siRNA Abolishes Ultraviolet Light-Induced Translesion Replication in HeLa Cells: A Comprehensive Study Using Alkaline Sucrose Density Gradient Sedimentation

    PubMed Central

    Takezawa, Jun; Ishimi, Yukio; Aiba, Naomi; Yamada, Kouichi

    2010-01-01

    When a replicative DNA polymerase stalls upon encountering a lesion on the template strand, it is relieved by other low-processivity polymerase(s), which insert nucleotide(s) opposite the lesion, extend by a few nucleotides, and dissociate from the 3′-OH. The replicative polymerase then resumes DNA synthesis. This process, termed translesion replication (TLS) or replicative bypass, may involve at least five different polymerases in mammals, although the participating polymerases and their roles have not been entirely characterized. Using siRNAs originally designed and an alkaline sucrose density gradient sedimentation technique, we verified the involvement of several polymerases in ultraviolet (UV) light-induced TLS in HeLa cells. First, siRNAs to Rev3 or Rev7 largely abolished UV-TLS, suggesting that these 2 gene products, which comprise Polζ, play a main role in mutagenic TLS. Second, Rev1-targeted siRNA also abrogated UV-TLS, indicating that Rev1 is also indispensable to mutagenic TLS. Third, Polη-targeted siRNA also prevented TLS to a greater extent than our expectations. Forth, although siRNA to Polι had no detectable effect, that to Polκ delayed UV-TLS. To our knowledge, this is the first study reporting apparent evidence for the participation of Polκ in UV-TLS. PMID:21151666

  5. Rev1, Rev3, or Rev7 siRNA Abolishes Ultraviolet Light-Induced Translesion Replication in HeLa Cells: A Comprehensive Study Using Alkaline Sucrose Density Gradient Sedimentation.

    PubMed

    Takezawa, Jun; Ishimi, Yukio; Aiba, Naomi; Yamada, Kouichi

    2010-12-01

    When a replicative DNA polymerase stalls upon encountering a lesion on the template strand, it is relieved by other low-processivity polymerase(s), which insert nucleotide(s) opposite the lesion, extend by a few nucleotides, and dissociate from the 3'-OH. The replicative polymerase then resumes DNA synthesis. This process, termed translesion replication (TLS) or replicative bypass, may involve at least five different polymerases in mammals, although the participating polymerases and their roles have not been entirely characterized. Using siRNAs originally designed and an alkaline sucrose density gradient sedimentation technique, we verified the involvement of several polymerases in ultraviolet (UV) light-induced TLS in HeLa cells. First, siRNAs to Rev3 or Rev7 largely abolished UV-TLS, suggesting that these 2 gene products, which comprise Polζ, play a main role in mutagenic TLS. Second, Rev1-targeted siRNA also abrogated UV-TLS, indicating that Rev1 is also indispensable to mutagenic TLS. Third, Polη-targeted siRNA also prevented TLS to a greater extent than our expectations. Forth, although siRNA to Polι had no detectable effect, that to Polκ delayed UV-TLS. To our knowledge, this is the first study reporting apparent evidence for the participation of Polκ in UV-TLS.

  6. Submarine landslides hazard offshore Israel

    NASA Astrophysics Data System (ADS)

    Katz, Oded

    2016-04-01

    Submarine landslides pose significant natural hazards. They can damage seafloor infrastructure, such as that used to recover oil and gas or seafloor telecommunication cables, and even generate tsunamis. We recently mapped 447 submarine landslides across the east Mediterranean continental slope, offshore Israel (hereafter the studied area). The mapped landslides are found at water depths of 130 m to 1,000 m and their volume ranges 10-5 - 100 km3. Landslide scars are typically related to a critical slope angle of >4° . Landslides at the northern part of the studied area are spatially associated with fault scarps and are smaller than the ones on the southern part. In this work we evaluate the potential hazard to population and to on- and off- shore facilities posed by submarine landslides across the studied area. We integrate three independent probabilities: (1) the probability for a landslide event of a given volume, based on the size distribution of the mapped landslides; (2) the probability for a landslide event in a given time, based on the reoccurrence time of triggering earthquakes with M >7, and on a 50,000 years general time frame derived from submarine landslides identified across the Mediterranean Sea; (3) the probability for a landslide event in a given area, based on the distribution of slopes exceeding the critical angle. Overall, the fraction of potentially destructive landslides (size > 0.1 km3) is small, 0.05. Thus, considering typical planning time scales of less than 100 years, the calculated hazard is only moderate. The small fraction of landslides with tsunamogenic potential (size > 1 km3), suggests that the hazard for landslide-induced tsunamis along the open slope part of the studied area is small. Landslides in the southern part of the studied area are larger and thus present a somewhat bigger potential source of tsunami waves.

  7. Rev-erb beta regulates the Srebp-1c promoter and mRNA expression in skeletal muscle cells

    SciTech Connect

    Ramakrishnan, Sathiya N.; Lau, Patrick; Crowther, Lisa M.; Cleasby, Mark E.; Millard, Susan; Leong, Gary M.; Cooney, Gregory J.; Muscat, George E.O.

    2009-10-30

    The nuclear hormone receptor, Rev-erb beta operates as a transcriptional silencer. We previously demonstrated that exogenous expression of Rev-erb{beta}{Delta}E in skeletal muscle cells increased Srebp-1c mRNA expression. We validated these in vitro observations by injection of an expression vector driving Rev-erb{beta}{Delta}E expression into mouse tibialis muscle that resulted in increased Srebp-1c mRNA expression. Paradoxically, Rev-erb{beta} siRNA expression in skeletal muscle cells repressed Srebp-1c expression, and indicated that Rev-erb{beta} expression was necessary for Srebp-1c expression. ChIP analysis demonstrated that Rev-erb{beta} was recruited to the Srebp-1c promoter. Moreover, Rev-erb{beta} trans-activated the Srebp-1c promoter, in contrast, Rev-erb{beta} efficiently repressed the Rev-erb{alpha} promoter, a previously characterized target gene. Finally, treatment with the Rev-erb agonist (hemin) (i) increased the trans-activation of the Srebp-1c promoter by Rev-erb{beta}; and (ii) increased Rev-erb{beta} and Srebp-1c mRNA expression. These data suggest that Rev-erb{beta} has the potential to activate gene expression, and is a positive regulator of Srebp-1c, a regulator of lipogenesis.

  8. Operational management of offshore energy assets

    NASA Astrophysics Data System (ADS)

    Kolios, A. J.; Martinez Luengo, M.

    2016-02-01

    Energy assets and especially those deployed offshore are subject to a variety of harsh operational and environmental conditions which lead to deterioration of their performance and structural capacity over time. The aim of reduction of CAPEX in new installations shifts focus to operational management to monitor and assess performance of critical assets ensuring their fitness for service throughout their service life and also to provide appropriate and effective information towards requalification or other end of life scenarios, optimizing the OPEX. Over the last decades, the offshore oil & gas industry has developed and applied various approaches in operational management of assets through Structural Health and Condition Monitoring (SHM/CM) systems which can be, at a certain level, transferable to offshore renewable installations. This paper aims to highlight the key differences between offshore oil & gas and renewable energy assets from a structural integrity and reliability perspective, provide a comprehensive overview of different approaches that are available and applicable, and distinguish the benefits of such systems in the efficient operation of offshore energy assets.

  9. Offshore oil gas trends in ROVs tooling

    SciTech Connect

    Jacobson, J.R. )

    1994-04-01

    The ROVs that operate in today's offshore environment bear little or no resemblance to those which first began supporting the oil and gas work requirements less than 15 years ago. In that short span of time, an explosion of subsea technology has occurred, rendering older equipment obsolete and expanding subsea remote intervention capabilities beyond the horizon of intervention tasks originally envisioned. Today's offshore work ROVs employ the latest in robotics, fiver optics, acoustics, video, and computer technologies, and routinely achieve better than 90 percent operational availability. Leading offshore ROV operations companies have demonstrated less than 1 percent down-time over thousands of hours of operation. As the offshore oil and gas market evolves, the ever-expanding capabilities of the work ROV plays a major role in shaping the operational concepts employed in subsea oil and gas field exploration, development, and production. This paper explores the capabilities of available ROV systems in use offshore today, the economic trends driving ROV technology development, and the new trends in the employment of ROVs and their associated sensors and tooling systems.

  10. New OBS network deployment offshore Ireland

    NASA Astrophysics Data System (ADS)

    Le Pape, Florian; Bean, Chris; Craig, David; Jousset, Philippe; Horan, Clare; Hogg, Colin; Donne, Sarah; McCann, Hannah; Möllhoff, Martin; Kirk, Henning; Ploetz, Aline

    2016-04-01

    With the presence of the stormy NE Atlantic, Ireland is ideally located to investigate further our understanding of ocean generated microseisms and use noise correlation methods to develop seismic imaging in marine environments as well as time-lapse monitoring. In order to study the microseismic activity offshore Ireland, 10 Broad Band Ocean Bottom Seismographs (OBSs) units including hydrophones have been deployed in January 2016 across the shelf offshore Donegal and out into the Rockall Trough. This survey represents the first Broadband passive study in this part of the NE Atlantic. The instruments will be recovered in August 2016 providing 8 months worth of data to study microseisms but also the offshore seismic activity in the area. One of the main goal of the survey is to investigate the spatial and temporal distributions of dominant microseism source regions, close to the microseism sources. Additionally we will study the coupling of seismic and acoustic signals at the sea bed and its evolution in both the deep water and continental shelf areas. Furthermore, the survey also aims to investigate further the relationship between sea state conditions (e.g. wave height, period), seafloor pressure variations and seismic data recorded on both land and seafloor. Finally, the deployed OBS network is also the first ever attempt to closely monitor local offshore earthquakes in Ireland. Ireland seismicity although relatively low can reduce slope stability and poses the possibility of triggering large offshore landslides and local tsunamis.

  11. A framework for offshore vendor capability development

    NASA Astrophysics Data System (ADS)

    Yusuf Wibisono, Yogi; Govindaraju, Rajesri; Irianto, Dradjad; Sudirman, Iman

    2016-02-01

    Offshore outsourcing is a common practice conducted by companies, especially in developed countries, by relocating one or more their business processes to other companies abroad, especially in developing countries. This practice grows rapidly owing to the ease of accessing qualified vendors with a lower cost. Vendors in developing countries compete more intensely to acquire offshore projects. Indonesia is still below India, China, Malaysia as main global offshore destinations. Vendor capability is among other factors that contribute to the inability of Indonesian vendor in competing with other companies in the global market. Therefore, it is essential to study how to increase the vendor's capability in Indonesia, in the context of global offshore outsourcing. Previous studies on the vendor's capability mainly focus on capabilities without considering the dynamic of capabilities due to the environmental changes. In order to be able to compete with competitors and maintain the competitive advantage, it is necessary for vendors to develop their capabilities continuously. The purpose of this study is to develop a framework that describes offshore vendor capability development along the client-vendor relationship stages. The framework consists of three main components, i.e. the stages of client-vendor relationship, the success of each stage, and the capabilities of vendor at each stage.

  12. REV-ERBα regulates Fgf21 expression in the liver via hepatic nuclear factor 6

    PubMed Central

    Chavan, Rohit; Preitner, Nadia; Okabe, Takashi; Strittmatter, Laureen Mansencal; Xu, Cheng; Ripperger, Jürgen A.; Pitteloud, Nelly

    2017-01-01

    ABSTRACT The circadian clock contributes to the timing of many body functions including metabolism and reproduction. The hepatokine fibroblast growth factor 21 (FGF21) is a critical metabolic regulator involved in modulation of fertility. Here we show that lack of the clock component REV-ERBα elevates FGF21 levels in liver and plasma. At the molecular level, REV-ERBα modulates the expression of FGF21 via the liver-specific hepatic nuclear factor 6 (HNF6). We conclude that REV-ERBα regulates metabolism and reproduction, at least in part, via regulation of Fgf21. PMID:27875243

  13. REV-ERBα regulates Fgf21 expression in the liver via hepatic nuclear factor 6.

    PubMed

    Chavan, Rohit; Preitner, Nadia; Okabe, Takashi; Strittmatter, Laureen Mansencal; Xu, Cheng; Ripperger, Jürgen A; Pitteloud, Nelly; Albrecht, Urs

    2017-01-15

    The circadian clock contributes to the timing of many body functions including metabolism and reproduction. The hepatokine fibroblast growth factor 21 (FGF21) is a critical metabolic regulator involved in modulation of fertility. Here we show that lack of the clock component REV-ERBα elevates FGF21 levels in liver and plasma. At the molecular level, REV-ERBα modulates the expression of FGF21 via the liver-specific hepatic nuclear factor 6 (HNF6). We conclude that REV-ERBα regulates metabolism and reproduction, at least in part, via regulation of Fgf21.

  14. Experimental verification of a real-time power curve for downregulated offshore wind power plants

    NASA Astrophysics Data System (ADS)

    Giebel, Gregor; Göcmen Bozkurt, Tuhfe; Sørensen, Poul; Rajczyk Skjelmose, Mads; Runge Kristoffersen, Jesper

    2015-04-01

    Wind farm scale experiments with wakes under downregulation have been initiated in Horns Rev wind farm in the frame of the PossPOW project (see posspow.dtu.dk). The experiments will be compared with the results of the calibrated GCLarsen wake model for real-time which is used not only to obtain real-time power curve but also to estimate the available power in wind farm level. Available (or Possible) Power is the power that a down-regulated (or curtailed) turbine or a wind power plant would produce if it were to operate in normal operational conditions and it is becoming more of particular interest due to increasing number of curtailment periods. Currently, the Transmission System Operators (TSOs) have no real way to determine exactly the available power of a down-regulated wind farm and the PossPOW project is addressing that need. What makes available power calculation interesting at the wind farm level is the change in the wake characteristics for different operational states. Even though the single turbine level available power is easily estimated, the sum of those signals from all turbines in a wind farm overestimates the power since the wake losses significantly decrease during curtailment. In order to calculate that effect, the turbine wind speed is estimated real-time from the produced power, the pitch angle and the rotor speed using a proximate Cp curve. A real-time wake estimation of normal operation is then performed and advected to the next downstream turbine, and so on until the entire wind farm is calculated. The estimation of the rotor effective wind speed, the parameterization of the GCLarsen wake model for real-time use (i.e., 1-sec data from Horns Rev and Thanet) and the details of the advection are the topic can be found in Göcmen et al. [1] Here we plan to describe the experiments using the Horns Rev wind farm and hopefully present the first validation results. Assuming similarity of the wind speeds between neighbouring rows of turbines, the

  15. FT-Raman spectroscopic study of keratotic materials: horn, hoof and tortoiseshell

    NASA Astrophysics Data System (ADS)

    Edwards, H. G. M.; Hunt, D. E.; Sibley, M. G.

    1998-05-01

    The Fourier-Transform Raman spectra of some mammalian and reptilian keratins, horn, hoof and tortoiseshell, have been analysed and used for the construction of a database for the identification of highly keratotic samples. The samples investigated were; bovine keratin and hoof, Texas Longhorn cattle horn, kudu horn, tortoiseshell and human finger nail. Significant spectral differences were observed in the 1000-400 cm -1 wavenumber range, which included the conformationally important ν(SS) and ν(CS) features around 500 and 640 cm -1, respectively. The amide I (1650 cm -1) and amide III (1260 cm -1) bands confirmed that the reptilian keratin studied exists in the β-sheet conformation, whilst mammalian keratins are predominantly laid down in an α-helical conformation. The FT-Raman spectral differences particularly between the horn and hoof specimens are very useful for the non-destructive characterisation of artefacts and provides a novel application of the technique.

  16. A high gain patch fed horn antenna for millimeter wave imaging receiver

    NASA Astrophysics Data System (ADS)

    Shireen, Rownak; Hwang, Timothy; Shi, Shouyuan; Prather, D. W.

    2005-11-01

    In this paper, antennas that combine transitions from microstrip line / coplanar waveguide (CPW) to horn antenna in a single unit are presented. Conventional single layer microstrip patch antennas inherently suffer narrow operation bandwidth; to widen the frequency bandwidth, stacked patch antennas are used and high gain is achieved from the horn antenna. Here, microstrip line / CPW directly feeds the bottom patch while the top patch couples parasitically to the bottom patch. For -10 dB return loss, 25% bandwidth is achieved for both microstrip line to horn antenna (MSLTHA) at center frequency f0=17.5 GHz and for CPW to horn antenna (CPWTHA) at f0=97 GHz. The designs were optimized using 3D Finite Element Method (FEM) software HFSS by Ansoft Corporation. The optimal design of MSLTHA has been fabricated and characterized. The return loss and far field radiation pattern are measured and has been found in very good agreement with the simulation results.

  17. Suppressing side-lobe radiations of horn antenna by loading metamaterial lens.

    PubMed

    Qi, Mei Qing; Tang, Wen Xuan; Ma, Hui Feng; Pan, Bai Cao; Tao, Zui; Sun, Yong Zhi; Cui, Tie Jun

    2015-03-13

    We propose a new approach to control the amplitude and phase distributions of electromagnetic fields over the aperture of a horn antenna. By loading a metamaterial lens inside the horn antenna, a tapered amplitude distribution of the aperture field is achieved, which can suppress the side-lobe radiations of the antenna. The metamaterial is further manipulated to achieve a flat phase distribution on the horn aperture to avoid the gain reduction that usually suffers in the conventional low-sidelobe antenna designs. A prototype of the metamaterial-loaded horn antenna is designed and fabricated. Both numerical simulations and measured results demonstrate the tapered aperture-field distribution and significant reduction of side-lobe and back-lobe radiations in the operating frequency band.

  18. Metabolic rate and evaporative water loss of Mexican Spotted and Great Horned Owls

    Treesearch

    Joseph L. Ganey; Russell P. Balda; Rudy M. King

    1993-01-01

    We measured rates of oxygen consumption and evaporative water loss (EWL) of Mexican Spotted (Strix occidentalis lucida) and Great Horned (Bubo virginianus) owls in Arizona. Basal metabolic rate averaged 0.84 ccO2. g-1. h-1...

  19. 12. NEAR FIELD HORN (TESTING DEVICE FOR EMIITER/ANTENNA ARRAY SYSTEM) ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    12. NEAR FIELD HORN (TESTING DEVICE FOR EMIITER/ANTENNA ARRAY SYSTEM) AT FACE "A" - VIEW IS LOOKING SOUTH 20° EAST. - Cape Cod Air Station, Massachusetts Military Reservation, Sandwich, Barnstable County, MA

  20. Giant Cutaneous Horn Overlying A Verruca at an Uncommon Site: Medical Marvel vs Superstitious Dilemma

    PubMed Central

    Sanjeeva, Karthik Kathladka; Ali, Puttur Sainuddin Mohammed Ameer; Rao, Srikar; Rai, Arvind Shivram

    2015-01-01

    Cutaneous horn has been a matter of discussion to mankind since time immemorial and a subject of fascination for the layman. There have been instances where certain groups saw it with angst and disgust, with a person having a large cutaneous horn on an exposed area getting a dismal look. We present a case of a 64-year-old man with a giant cutaneous horn over his left gluteal region. Cutaneous horns more commonly present in the sun-exposed areas. In our case it has presented in an uncommon site. The patient had delayed and denied medical treatment due to his superstitious beliefs, after having sought advice from faith healers leading to progression of the disease. This case has been presented for its giant size (rare variety), its location being over the gluteal region (photo-protected site) and its benign histopathology suggestive of wart in spite of the giant size. PMID:26023596

  1. Giant Cutaneous Horn Overlying A Verruca at an Uncommon Site: Medical Marvel vs Superstitious Dilemma.

    PubMed

    Sanjeeva, Karthik Kathladka; Ali, Puttur Sainuddin Mohammed Ameer; Pinto, Malcolm; Rao, Srikar; Rai, Arvind Shivram

    2015-04-01

    Cutaneous horn has been a matter of discussion to mankind since time immemorial and a subject of fascination for the layman. There have been instances where certain groups saw it with angst and disgust, with a person having a large cutaneous horn on an exposed area getting a dismal look. We present a case of a 64-year-old man with a giant cutaneous horn over his left gluteal region. Cutaneous horns more commonly present in the sun-exposed areas. In our case it has presented in an uncommon site. The patient had delayed and denied medical treatment due to his superstitious beliefs, after having sought advice from faith healers leading to progression of the disease. This case has been presented for its giant size (rare variety), its location being over the gluteal region (photo-protected site) and its benign histopathology suggestive of wart in spite of the giant size.

  2. Suppressing Side-Lobe Radiations of Horn Antenna by Loading Metamaterial Lens

    PubMed Central

    Qi, Mei Qing; Tang, Wen Xuan; Ma, Hui Feng; Pan, Bai Cao; Tao, Zui; Sun, Yong Zhi; Cui, Tie Jun

    2015-01-01

    We propose a new approach to control the amplitude and phase distributions of electromagnetic fields over the aperture of a horn antenna. By loading a metamaterial lens inside the horn antenna, a tapered amplitude distribution of the aperture field is achieved, which can suppress the side-lobe radiations of the antenna. The metamaterial is further manipulated to achieve a flat phase distribution on the horn aperture to avoid the gain reduction that usually suffers in the conventional low-sidelobe antenna designs. A prototype of the metamaterial-loaded horn antenna is designed and fabricated. Both numerical simulations and measured results demonstrate the tapered aperture-field distribution and significant reduction of side-lobe and back-lobe radiations in the operating frequency band. PMID:25766083

  3. Development and operational experience of magnetic horn system for T2K experiment

    NASA Astrophysics Data System (ADS)

    Sekiguchi, T.; Bessho, K.; Fujii, Y.; Hagiwara, M.; Hasegawa, T.; Hayashi, K.; Ishida, T.; Ishii, T.; Kobayashi, H.; Kobayashi, T.; Koike, S.; Koseki, K.; Maruyama, T.; Matsumoto, H.; Nakadaira, T.; Nakamura, K.; Nakayoshi, K.; Nishikawa, K.; Oyama, Y.; Sakashita, K.; Shibata, M.; Suzuki, Y.; Tada, M.; Takahashi, K.; Tsukamoto, T.; Yamada, Y.; Yamanoi, Y.; Yamaoka, H.; Ichikawa, A. K.; Kubo, H.; Butcher, Z.; Coleman, S.; Missert, A.; Spitz, J.; Zimmerman, E. D.; Tzanov, M.; Bartoszek, L.

    2015-07-01

    A magnetic horn system to be operated at a pulsed current of 320 kA and to survive high-power proton beam operation at 750 kW was developed for the T2K experiment. The first set of T2K magnetic horns was operated for over 12 million pulses during the four years of operation from 2010 to 2013, under a maximum beam power of 230 kW, and 6.63×1020 protons were exposed to the production target. No significant damage was observed throughout this period. This successful operation of the T2K magnetic horns led to the discovery of the νμ →νe oscillation phenomenon in 2013 by the T2K experiment. In this paper, details of the design, construction, and operation experience of the T2K magnetic horns are described.

  4. Federal Offshore Statistics, 1993. Leasing, exploration, production, and revenue as of December 31, 1993

    SciTech Connect

    Francois, D.K.

    1994-12-31

    This document contains statistical data on the following: federal offshore lands; offshore leasing activity and status; offshore development activity; offshore production of crude oil and natural gas; federal offshore oil and natural gas sales volume and royalties; revenue from federal offshore leases; disbursement of federal offshore revenue; reserves and resource estimates of offshore oil and natural gas; oil pollution in US and international waters; and international activities and marine minerals. A glossary is included.

  5. Federal offshore statistics: leasing - exploration - production - revenue

    SciTech Connect

    Essertier, E.P.

    1984-01-01

    Federal Offshore Statistics is a numerical record of what has happened since Congress gave authority to the Secretary of the Interior in 1953 to lease the Federal portion of the Continental Shelf for oil and gas. The publication updates and augments the first Federal Offshore Statistics, published in December 1983. It also extends a statistical series published annually from 1969 until 1981 by the US Geological Survey (USGS) under the title Outer Continental Shelf Statistics. The USGS collected royalties and supervised operation and production of minerals on the Outer Continental Shelf (OCS) until the Minerals Management Service (MMS) took over these functions in 1982. Statistics are presented under the following topics: (1) highlights, (2) leasing, (3) exploration and development, (4) production and revenue, (5) federal offshore production by ranking operator, 1983, (6) reserves and undiscovered recoverable resources, and (7) oil pollution in the world's oceans.

  6. Solar power satellite offshore rectenna study

    NASA Astrophysics Data System (ADS)

    1980-05-01

    Offshore rectennas are feasible and cost competitive with land rectennas but the type of rectenna suitable for offshore use is quite different from that specified in the present reference system. A nonground plane design minimizes the weight and greatly reduces the number of costly support towers. This perferred design is an antenna array consisting of individually encapsulated dipoles with reflectors or tagis supported on feed wires. Such a 5 GW rectenna could be built at a 50 m water depth site to withstand hurricane, winter storm, and icing conditions for a one time cost of $5.7 billion. Subsequent units would be about 1.3 less expensive. More benign and more shallow water sites would result in substantially lower costs. The major advantage of an offshore rectenna is the removal of microwave radiation from populated areas.

  7. Model refinement for offshore platforms: Experimental study

    NASA Astrophysics Data System (ADS)

    Zhang, Min; Chen, Zongli; Wu, Yanjian

    2017-08-01

    Offshore jacket platforms are widely used in offshore oil and gas exploitation. Finite element models of such structures need to have many degrees of freedom (DOFs) to represent the geometrical detail of complex structures, thereby leading to incompatibility in the number of DOFs of experimental models. To bring them both to the same order while ensuring that the essential eigen- properties of the refined model match those of experimental models, an extended model refinement procedure is presented in this paper. Vibration testing of an offshore jacket platform model is performed to validate the applicability of the proposed approach. A full-order finite element model of the platform is established and then tuned to meet the measured modal properties identified from the acceleration signals. Both model reduction and modal expansion methods are investigated, as well as various scenarios of sensor arrangements. Upon completion of the refinement, the updated jacket platform model matches the natural frequencies of the measured model well.

  8. Solar power satellite offshore rectenna study

    NASA Technical Reports Server (NTRS)

    1980-01-01

    Offshore rectennas are feasible and cost competitive with land rectennas but the type of rectenna suitable for offshore use is quite different from that specified in the present reference system. A nonground plane design minimizes the weight and greatly reduces the number of costly support towers. This perferred design is an antenna array consisting of individually encapsulated dipoles with reflectors or tagis supported on feed wires. Such a 5 GW rectenna could be built at a 50 m water depth site to withstand hurricane, winter storm, and icing conditions for a one time cost of $5.7 billion. Subsequent units would be about 1.3 less expensive. More benign and more shallow water sites would result in substantially lower costs. The major advantage of an offshore rectenna is the removal of microwave radiation from populated areas.

  9. Current and future offshore activities in Canada

    SciTech Connect

    Hnatiuk, J.

    1984-05-01

    The development of innovative exploratory drilling systems for Canada's harsh Arctic offshore areas over the past decade is described. Future activity in these areas, including possible production concepts, is also discussed. The results of the experience in Canadian waters can be applied in other Arctic areas of the world including offshore Alaska. This operating experience will serve to further advance the drilling technology and will serve as a basis for the design of Arctic offshore production and transportation systems. Artificial islands, first commenced in 1972, are still being constructed but with improved designs and equipment. A step forward has been the use of subsea berms on which concrete or steel segmented caissons have been placed. Integrated-type steel caissons have also been adapted for placement on subsea berms, one of which is half of a crude oil tanker and a second, a purpose-built steel caisson to be placed this summer.

  10. Entrapment of the Temporal Horn as a Cause of Pure Wernicke Aphasia: Case Report

    PubMed Central

    Spallone, Aldo; Belvisi, Daniele; Marsili, Luca

    2015-01-01

    Entrapment of the temporal horn is an extremely rare pathologic condition occurring as a result of surgery for tumors, intraventricular infections, hemorrhage, or traumatic events involving the peritrigonal area. We report a case of a 58-year-old man who presented with pure Wernicke aphasia (never described before in the albeit rare cases of isolated temporal horn dilatation) that regressed completely following successful ventriculoperitoneal shunting. The relevant literature is also briefly reviewed. PMID:26251784

  11. Properties of cutoff corrugated surfaces for corrugated horn design. [corrugation shape and density effects on scattering

    NASA Technical Reports Server (NTRS)

    Mentzer, C. A.; Peters, L., Jr.

    1974-01-01

    Corrugated horns involve a junction between the corrugated surface and a conducting ground plane. Proper horn design requires an understanding of the electromagnetic properties of the corrugated surface and this junction. An integral equation solution has been used to study the influence of corrugation density and tooth thickness on the power loss, surface current, and the scattering from a ground plane/corrugated surface junction.

  12. Single-unit analysis of the spinal dorsal horn in patients with neuropathic pain.

    PubMed

    Guenot, Marc; Bullier, Jean; Rospars, Jean-Pierre; Lansky, Petr; Mertens, Patrick; Sindou, Marc

    2003-04-01

    Despite the key role played by the dorsal horn of the spinal cord in pain modulation, single-unit recordings have only been performed very rarely in this structure in humans. The authors report the results of a statistical analysis of 64 unit recordings from the human dorsal horn. The recordings were done in three groups of patients: patients with deafferentation pain resulting from brachial plexus avulsion, patients with neuropathic pain resulting from peripheral nerve injury, and patients with pain resulting from disabling spasticity. The patterns of neuronal activities were compared among these three groups. Nineteen neurons were recorded in the dorsal horns of five patients undergoing DREZotomy for a persistent pain syndrome resulting from peripheral nerve injury (i.e., nondeafferented dorsal horns), 31 dorsal horn neurons were recorded in nine patients undergoing DREZotomy for a persistent pain syndrome resulting from brachial plexus avulsion (i.e., deafferented dorsal horns), and 14 neurons were recorded in eight patients undergoing DREZotomy for disabling spasticity. These groups were compared in terms of mean frequency, coefficient of variation of the discharge, other properties of the neuronal discharge studied by the nonparametric test of Wald-Wolfowitz, and the possible presence of bursts. The coefficient of variation tended to be higher in the deafferented dorsal horn group than in the other two groups. Two neurons displaying burst activity could be recorded, both of which belonged to the deafferented dorsal horn group. A significant difference was found in term of neuronal behavior between the peripheral nerve trauma group and the other groups: The brachial plexus avulsion and disabling spasticity groups were very similar, including various types of neuronal behavior, whereas the peripheral nerve lesion group included mostly neurons with "nonrandom" patterns of discharge (i.e., with serial dependency of interspike intervals).

  13. The complete mitochondrial genome of scimitar-horned oryx (Oryx dammah).

    PubMed

    Zhang, Honghai; Ren, Ying; Chen, Lei; Sha, Weilai

    2012-10-01

    In this study, a blood sample was obtained from a scimitar-horned oryx in Jinan Paomaling World of Wildlife and the complete nucleotide sequence of scimitar-horned oryx mitochondrial genome was determined. Sequence analysis showed that the genome structure was in accordance with other bovid species that it contained 22 tRNA genes, 12S rRNA gene, 16S rRNA gene, 13 protein-coding genes and 1 control region.

  14. Neurodegeneration in the Spinal Ventral Horn Prior to Motor Impairment in Cervical Spondylotic Myelopathy.

    PubMed

    Grabher, Patrick; Mohammadi, Siawoosh; David, Gergely; Freund, Patrick

    2017-08-01

    Remote gray matter pathology has been suggested rostral to the compression site in cervical spondylotic myelopathy (CSM). We therefore assessed neurodegeneration in the gray matter ventral and dorsal horns. Twenty patients with CSM and 18 healthy subjects underwent a high-resolution structural and diffusion magnetic resonance imaging protocol at vertebra C2/C3. Patients received comprehensive clinical assessments. T2*-weighted data provided cross-sectional area measurements of gray matter ventral and dorsal horns to identify atrophy. At the identical location, mean diffusivity (MD) and fractional anisotropy (FA) determined the microstructural integrity. Finally, the relationships between neurodegeneration occurring in the gray and white matter and clinical impairment were investigated. Patients suffered from mild-to-moderate CSM with mainly sensory impairment. In the ventral horns, cross-sectional area was not reduced (p = 0.863) but MD was increased (p = 0.045). The magnitude of MD changes within the ventral horn was associated with white matter diffusivity changes (MD: p = 0.013; FA: p = 0.028) within the lateral corticospinal tract. In contrast, dorsal horn cross-sectional area was reduced by 16.0% (p < 0.001) without alterations in diffusivity indices, compared with controls. No associations between the magnitude of ventral and dorsal horn neurodegeneration and clinical impairment were evident. Focal cord gray matter pathology is evident remote to the compression site in vivo in CSM patients. Microstructural changes in the ventral horns (i.e., motoneurons) related to corticospinal tract integrity in the absence of atrophy and marked motor impairment. Dorsal horn atrophy corresponded to main clinical representation of sensory impairment. Thus, neuroimaging biomarkers of cord gray matter integrity reveal focal neurodegeneration prior to marked clinical impairment and thus could serve as predictors of ensuing impairment in CSM patients.

  15. National Offshore Wind Energy Grid Interconnection Study Full Report

    SciTech Connect

    Daniel, John P.; Liu, Shu; Ibanez, Eduardo; Pennock, Ken; Reed, Gregory; Hanes, Spencer

    2014-07-30

    The National Offshore Wind Energy Grid Interconnection Study (NOWEGIS) considers the availability and potential impacts of interconnecting large amounts of offshore wind energy into the transmission system of the lower 48 contiguous United States.

  16. Strengthening America's Energy Security with Offshore Wind (Fact Sheet)

    SciTech Connect

    Not Available

    2012-02-01

    This fact sheet describes the current state of the offshore wind industry in the United States and the offshore wind research and development activities conducted the U.S. Department of Energy Wind and Water Power Program.

  17. National Offshore Wind Energy Grid Interconnection Study Executive Summary

    SciTech Connect

    Daniel, John P.; Liu, Shu; Ibanez, Eduardo; Pennock, Ken; Reed, Gregory; Hanes, Spencer

    2014-07-30

    The National Offshore Wind Energy Grid Interconnection Study (NOWEGIS) considers the availability and potential impacts of interconnecting large amounts of offshore wind energy into the transmission system of the lower 48 contiguous United States.

  18. Moisture, anisotropy, stress state, and strain rate effects on bighorn sheep horn keratin mechanical properties

    DOE PAGES

    Johnson, K. L.; Trim, M. W.; Francis, D. K.; ...

    2016-10-01

    Our paper investigates the effects of moisture, anisotropy, stress state, and strain rate on the mechanical properties of the bighorn sheep (Ovis Canadensis) horn keratin. The horns consist of fibrous keratin tubules extending along the length of the horn and are contained within an amorphous keratin matrix. We tested samples in the rehydrated (35 wt.% water) and ambient dry (10 wt.% water) conditions along the longitudinal and radial directions under tension and compression. Increased moisture content was found to increase ductility and decrease strength, as well as alter the stress state dependent nature of the material. Furthermore, the horn keratinmore » demonstrates a significant strain rate dependence in both tension and compression, and also showed increased energy absorption in the hydrated condition at high strain rates when compared to quasi-static data, with increases of 114% in tension and 192% in compression. Compressive failure occurred by lamellar buckling in the longitudinal orientation followed by shear delamination. Tensile failure in the longitudinal orientation occurred by lamellar delamination combined with tubule pullout and fracture. Finally, the structure-property relationships quantified here for bighorn sheep horn keratin can be used to help validate finite element simulations of ram’s impacting each other as well as being useful for other analysis regarding horn keratin on other animals.« less

  19. Moisture, anisotropy, stress state, and strain rate effects on bighorn sheep horn keratin mechanical properties

    SciTech Connect

    Johnson, K. L.; Trim, M. W.; Francis, D. K.; Whittington, W. R.; Miller, J. A.; Bennett, C. E.; Horstemeyer, M. F.

    2016-10-01

    Our paper investigates the effects of moisture, anisotropy, stress state, and strain rate on the mechanical properties of the bighorn sheep (Ovis Canadensis) horn keratin. The horns consist of fibrous keratin tubules extending along the length of the horn and are contained within an amorphous keratin matrix. We tested samples in the rehydrated (35 wt.% water) and ambient dry (10 wt.% water) conditions along the longitudinal and radial directions under tension and compression. Increased moisture content was found to increase ductility and decrease strength, as well as alter the stress state dependent nature of the material. Furthermore, the horn keratin demonstrates a significant strain rate dependence in both tension and compression, and also showed increased energy absorption in the hydrated condition at high strain rates when compared to quasi-static data, with increases of 114% in tension and 192% in compression. Compressive failure occurred by lamellar buckling in the longitudinal orientation followed by shear delamination. Tensile failure in the longitudinal orientation occurred by lamellar delamination combined with tubule pullout and fracture. Finally, the structure-property relationships quantified here for bighorn sheep horn keratin can be used to help validate finite element simulations of ram’s impacting each other as well as being useful for other analysis regarding horn keratin on other animals.

  20. a Design of the Driver Airbag Module with Floating Horn Assembly

    NASA Astrophysics Data System (ADS)

    Suh, Chang-Min; Lee, Young-Hoon; Suh, Duck-Young

    The driver airbag system is designed as a supplemental restraint system in addition to the seatbelt, and is designed to protect the driver's head and chest against severe injury by a device that is actuated in case of vehicle's fronted impact. Deployment of an airbag module with floating horn assembly is a highly dynamic process. The concept of driver airbag module with floating horn assembly and aluminum emblem is presented as a useful parameter when the airbag deploys and the energy is evaluated as performance factor in airbag module. Floating horn assembly is also one of the major factors for driver airbag module design to perform its horn function and check the package between driver airbag module and steering wheel. This study on the design of driver airbag module with floating horn assembly proved the feasibility as a new safety device. However, the system level study is needed for decrease of passenger injury. This study can be used for the implementation of a prototype of DABM with floating horn device.

  1. Hyaluronic acid membrane for reducing adhesion formation and reformation in the rat uterine horn.

    PubMed

    Yarali, H; Zahradka, B F; Gomel, V

    1994-09-01

    The efficacy of hyaluronic acid (HA) membrane in preventing or reducing intraperitoneal adhesion formation and reformation was evaluated in the rat uterine horn. Forty-seven Wistar rats were employed. Following a measured laser injury on the right uterine horn of each rat, HA membrane was applied to cover the site of injury in 20 (HA membrane group). No membrane was applied in another 20 (control group). The type and extent of adhesions were assessed at relaparotomy. Following microsurgical adhesiolysis at second-look laparotomy, the same animals were randomized to the HA membrane and control groups. The type and extent of adhesion reformation were evaluated at third-look laparotomy. Following a similar injury on the right uterine horn in another seven rats, HA membrane was applied on both uterine horns. A repeat laparotomy was performed three hours later to assess the status of the membrane. The type and extent of adhesion formation and reformation were comparable between the HA membrane and control groups. The HA membrane did not remain on the uterine horn and gelled rapidly. Hyaluronic acid membrane was ineffective in reducing adhesion formation and reformation in the rat uterine horn.

  2. Ruptured Rudimentary Horn Pregnancy Diagnosed by Preoperative Magnetic Resonance Imaging Resulting in Fetal Salvage

    PubMed Central

    Pillai, Silja A.; Mathew, Mariam; Ishrat, Noreen; Kakaria, Anupam; Qureshi, Asim; Vaidyanathan, Gowri

    2015-01-01

    Pregnancy in a rudimentary horn is very rare. The rupture of the horn during pregnancy is an obstetric emergency which can be life-threatening for both the mother and fetus. Preoperative diagnosis of such pregnancies can be challenging and they are usually diagnosed intraoperatively. We report a unique case of a 31-year-old multiparous woman who presented to the Sultan Qaboos University Hospital in Muscat, Oman, in January 2013 at 32 gestational weeks with abdominal pain. Ultrasonography was inconclusive. A rudimentary horn pregnancy was subsequently diagnosed via magnetic resonance imaging (MRI). An emergency laparotomy revealed haemoperitoneum and a ruptured rudimentary horn pregnancy. A live baby with an Apgar score of 2 at one minute and 7 at five minutes was delivered. The rudimentary horn with the placenta in situ was excised and a left salpingo-oophorectomy was performed. The postoperative period was uneventful. The authors recommend MRI as an excellent diagnostic modality to confirm rudimentary horn pregnancies and to expedite appropriate management. PMID:26357563

  3. Moisture, anisotropy, stress state, and strain rate effects on bighorn sheep horn keratin mechanical properties

    SciTech Connect

    Johnson, K. L.; Trim, M. W.; Francis, D. K.; Whittington, W. R.; Miller, J. A.; Bennett, C. E.; Horstemeyer, M. F.

    2016-10-01

    Our paper investigates the effects of moisture, anisotropy, stress state, and strain rate on the mechanical properties of the bighorn sheep (Ovis Canadensis) horn keratin. The horns consist of fibrous keratin tubules extending along the length of the horn and are contained within an amorphous keratin matrix. We tested samples in the rehydrated (35 wt.% water) and ambient dry (10 wt.% water) conditions along the longitudinal and radial directions under tension and compression. Increased moisture content was found to increase ductility and decrease strength, as well as alter the stress state dependent nature of the material. Furthermore, the horn keratin demonstrates a significant strain rate dependence in both tension and compression, and also showed increased energy absorption in the hydrated condition at high strain rates when compared to quasi-static data, with increases of 114% in tension and 192% in compression. Compressive failure occurred by lamellar buckling in the longitudinal orientation followed by shear delamination. Tensile failure in the longitudinal orientation occurred by lamellar delamination combined with tubule pullout and fracture. Finally, the structure-property relationships quantified here for bighorn sheep horn keratin can be used to help validate finite element simulations of ram’s impacting each other as well as being useful for other analysis regarding horn keratin on other animals.

  4. Transient amnesia after perimesencephalic haemorrhage: the role of enlarged temporal horns

    PubMed Central

    Hop, J.; Brilstra, E.; Rinkel, G.

    1998-01-01

    OBJECTIVES—Although long term outcome of patients with perimesencephalic haemorrhage, a benign subset of subarachnoid haemorrhage, is excellent, some patients report an episode of amnesia for the first hours to days after the ictus. The relation between the occurrence of amnesia and the size of the ventricles on CT, including the temporal horns, were studied in patients with perimesencephalic haemorrhage.
METHODS—Twenty seven consecutive patients with perimesencephalic haemorrhage were asked about the occurrence of amnesia. Age adjusted bicaudate indices and third ventricle sizes were calculated. Linear measurements of the temporal horn were taken in three directions: anterior-posterior, medial-lateral, and oblique. Additionally, enlargement of the temporal horns was assessed with the "naked eye".
RESULTS—Ten of the 27 patients reported an episode of transient amnesia; in these patients the mean sizes of the temporal horns were larger than in patients without amnesia, ranging from a factor of 1.7 for the medial-lateral measurement to a factor of 2.3 for the anterior-posterior measurement. Most of the patients with amnesia had relative bicaudate indices and relative third ventricle sizes> 1, and all had enlarged temporal horns at "naked eye" assessment.
CONCLUSION—About one third of patients with perimesencephalic haemorrhage have an episode of amnesia shortly after the bleed. The occurrence of amnesia is associated with enlargement of the temporal horns, and might be explained by temporary hippocampal dysfunction.

 PMID:9771795

  5. A progesterone antagonist cannot prevent fetal survival if the uterine horn is incised.

    PubMed

    Tamada, H; Inaba, T; Sawada, T

    1998-12-01

    The fetuses released into the abdominal cavity by uterine incision escape from most physical influences of the uterus. This study examined whether these fetuses require progesterone actions for survival during late pregnancy in rats. A longitudinal incision in one uterine horn (with the other horn intact) together with bilateral ovariectomy (OVX), removal of the main progesterone-production sites, or sham OVX, were performed on day 18 of pregnancy. Thereafter the rats were given daily subcutaneous injections of anti-progesterone RU 486 (10 mg/kg), or vehicle alone, and the fetal survival rate in each uterine horn was examined on day 21. In those controls which received sham OVX plus injections of vehicle, fetal survival rates were more than 80% in both uterine horns. In the other groups, which received sham OVX plus injections of RU 486, or OVX plus injections of vehicle, or OVX plus injections of RU 486, the fetal survival rates in the intact uterine horns were 4%, 0% and 0%, respectively. In the incised uterine horns of these groups, however, the fetal survival rates were 59%, 67% and 56%, respectively. The results suggest that progesterone, which is required for maintaining pregnancy, may not be essential for survival of fetuses released into the abdominal cavity. Progesterone actions unrelated to uterine physical environment are likely to be dispensable for fetal survival during late pregnancy in rats.

  6. Insights into the Development and Evolution of Exaggerated Traits Using De Novo Transcriptomes of Two Species of Horned Scarab Beetles

    PubMed Central

    Warren, Ian A.; Vera, J. Cristobal; Johns, Annika; Zinna, Robert; Marden, James H.; Emlen, Douglas J.; Dworkin, Ian; Lavine, Laura C.

    2014-01-01

    Scarab beetles exhibit an astonishing variety of rigid exo-skeletal outgrowths, known as “horns”. These traits are often sexually dimorphic and vary dramatically across species in size, shape, location, and allometry with body size. In many species, the horn exhibits disproportionate growth resulting in an exaggerated allometric relationship with body size, as compared to other traits, such as wings, that grow proportionately with body size. Depending on the species, the smallest males either do not produce a horn at all, or they produce a disproportionately small horn for their body size. While the diversity of horn shapes and their behavioural ecology have been reasonably well studied, we know far less about the proximate mechanisms that regulate horn growth. Thus, using 454 pyrosequencing, we generated transcriptome profiles, during horn growth and development, in two different scarab beetle species: the Asian rhinoceros beetle, Trypoxylus dichotomus, and the dung beetle, Onthophagus nigriventris. We obtained over half a million reads for each species that were assembled into over 6,000 and 16,000 contigs respectively. We combined these data with previously published studies to look for signatures of molecular evolution. We found a small subset of genes with horn-biased expression showing evidence for recent positive selection, as is expected with sexual selection on horn size. We also found evidence of relaxed selection present in genes that demonstrated biased expression between horned and horn-less morphs, consistent with the theory of developmental decoupling of phenotypically plastic traits. PMID:24586317

  7. Regional method to assess offshore slope stability.

    USGS Publications Warehouse

    Lee, H.J.; Edwards, B.D.

    1986-01-01

    The slope stability of some offshore environments can be evaluated by using only conventional acoustic profiling and short-core sampling, followed by laboratory consolidation and strength testing. The test results are synthesized by using normalized-parameter techniques. The normalized data are then used to calculate the critical earthquake acceleration factors or the wave heights needed to initiate failure. These process-related parameters provide a quantitative measure of the relative stability for locations from which short cores were obtained. The method is most applicable to offshore environments of gentle relief and simple subsurface structure and is not considered a substitute for subsequent site-specific analysis. -from ASCE Publications Information

  8. 78 FR 27971 - Determination That REV-EYES (Dapiprazole Hydrochloride Ophthalmic Solution), 0.5%, Was Not...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-05-13

    ... HUMAN SERVICES Food and Drug Administration Determination That REV-EYES (Dapiprazole Hydrochloride... determined that REV-EYES (dapiprazole hydrochloride ophthalmic solution), 0.5%, was not withdrawn from sale... refer to a listed drug. REV-EYES (dapiprazole hydrochloride ophthalmic solution), 0.5%, is the...

  9. 75 FR 69741 - Proposed Collection; Comment Request for Rev. Proc. 2007-99 (RP-127367-07), 9100 Relief Under...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-11-15

    ... Request for Rev. Proc. 2007-99 (RP- 127367-07), 9100 Relief Under Sections 897 and 1445 AGENCY: Internal...(c)(2)(A)). Currently, the IRS is soliciting comments concerning Rev. Proc. 2007-99 (RP-127367-07... INFORMATION: Title: Rev. Proc. 2007-99 (RP-127367-07), 9100 Relief Under Sections 897 and 1445. OMB Number...

  10. FANCD2 and REV1 cooperate in the protection of nascent DNA strands in response to replication stress

    PubMed Central

    Yang, Yeran; Liu, Zhenbo; Wang, Fengli; Temviriyanukul, Piya; Ma, Xiaolu; Tu, Yingfeng; Lv, Lingna; Lin, Yu-Fen; Huang, Min; Zhang, Ting; Pei, Huadong; Chen, Benjamin P.C.; Jansen, Jacob G.; de Wind, Niels; Fischhaber, Paula L.; Friedberg, Errol C.; Tang, Tie-Shan; Guo, Caixia

    2015-01-01

    REV1 is a eukaryotic member of the Y-family of DNA polymerases involved in translesion DNA synthesis and genome mutagenesis. Recently, REV1 is also found to function in homologous recombination. However, it remains unclear how REV1 is recruited to the sites where homologous recombination is processed. Here, we report that loss of mammalian REV1 results in a specific defect in replication-associated gene conversion. We found that REV1 is targeted to laser-induced DNA damage stripes in a manner dependent on its ubiquitin-binding motifs, on RAD18, and on monoubiquitinated FANCD2 (FANCD2-mUb) that associates with REV1. Expression of a FANCD2-Ub chimeric protein in RAD18-depleted cells enhances REV1 assembly at laser-damaged sites, suggesting that FANCD2-mUb functions downstream of RAD18 to recruit REV1 to DNA breaks. Consistent with this suggestion we found that REV1 and FANCD2 are epistatic with respect to sensitivity to the double-strand break-inducer camptothecin. REV1 enrichment at DNA damage stripes also partially depends on BRCA1 and BRCA2, components of the FANCD2/BRCA supercomplex. Intriguingly, analogous to FANCD2-mUb and BRCA1/BRCA2, REV1 plays an unexpected role in protecting nascent replication tracts from degradation by stabilizing RAD51 filaments. Collectively these data suggest that REV1 plays multiple roles at stalled replication forks in response to replication stress. PMID:26187992

  11. FANCD2 and REV1 cooperate in the protection of nascent DNA strands in response to replication stress.

    PubMed

    Yang, Yeran; Liu, Zhenbo; Wang, Fengli; Temviriyanukul, Piya; Ma, Xiaolu; Tu, Yingfeng; Lv, Lingna; Lin, Yu-Fen; Huang, Min; Zhang, Ting; Pei, Huadong; Chen, Benjamin P C; Jansen, Jacob G; de Wind, Niels; Fischhaber, Paula L; Friedberg, Errol C; Tang, Tie-Shan; Guo, Caixia

    2015-09-30

    REV1 is a eukaryotic member of the Y-family of DNA polymerases involved in translesion DNA synthesis and genome mutagenesis. Recently, REV1 is also found to function in homologous recombination. However, it remains unclear how REV1 is recruited to the sites where homologous recombination is processed. Here, we report that loss of mammalian REV1 results in a specific defect in replication-associated gene conversion. We found that REV1 is targeted to laser-induced DNA damage stripes in a manner dependent on its ubiquitin-binding motifs, on RAD18, and on monoubiquitinated FANCD2 (FANCD2-mUb) that associates with REV1. Expression of a FANCD2-Ub chimeric protein in RAD18-depleted cells enhances REV1 assembly at laser-damaged sites, suggesting that FANCD2-mUb functions downstream of RAD18 to recruit REV1 to DNA breaks. Consistent with this suggestion we found that REV1 and FANCD2 are epistatic with respect to sensitivity to the double-strand break-inducer camptothecin. REV1 enrichment at DNA damage stripes also partially depends on BRCA1 and BRCA2, components of the FANCD2/BRCA supercomplex. Intriguingly, analogous to FANCD2-mUb and BRCA1/BRCA2, REV1 plays an unexpected role in protecting nascent replication tracts from degradation by stabilizing RAD51 filaments. Collectively these data suggest that REV1 plays multiple roles at stalled replication forks in response to replication stress.

  12. 75 FR 10524 - NUREG-0654/FEMA-REP-1, Rev. 1, Supplement 3, Guidance for Protective Action Recommendations for...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-03-08

    ... COMMISSION NUREG-0654/FEMA-REP-1, Rev. 1, Supplement 3, Guidance for Protective Action Recommendations for... document entitled: ``NUREG-0654/FEMA-REP-1, Rev. 1, Supplement 3, Guidance for Protective Action...-4737, or by e-mail to pdr.resource@nrc.gov . NUREG-0654/FEMA-REP-1, Rev. 1, Supplement 3,...

  13. 75 FR 27840 - NUREG-0654/FEMA-REP-1, Rev. 1, Supplement 3, Guidance for Protective Action Recommendations for...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-05-18

    ...: 2010-11842] NUCLEAR REGULATORY COMMISSION [NRC-2010-0080] NUREG-0654/FEMA-REP-1, Rev. 1, Supplement 3...: ``NUREG-0654/FEMA-REP-1, Rev. 1, Supplement 3, Guidance for Protective Action Recommendations for General... the existing guidance contained in Supplement 3 to NUREG- 0654/FEMA-REP-1, Rev. 1, ``Criteria...

  14. Inhibition of REV3 Expression Induces Persistent DNA Damage and Growth Arrest in Cancer Cells12

    PubMed Central

    Knobel, Philip A; Kotov, Ilya N; Felley-Bosco, Emanuela; Stahel, Rolf A; Marti, Thomas M

    2011-01-01

    REV3 is the catalytic subunit of DNA translesion synthesis polymerase ζ. Inhibition of REV3 expression increases the sensitivity of human cells to a variety of DNA-damaging agents and reduces the formation of resistant cells. Surprisingly, we found that short hairpin RNA-mediated depletion of REV3 per se suppresses colony formation of lung (A549, Calu-3), breast (MCF-7, MDA-MB-231), mesothelioma (IL45 and ZL55), and colon (HCT116 +/-p53) tumor cell lines, whereas control cell lines (AD293, LP9-hTERT) and the normal mesothelial primary culture (SDM104) are less affected. Inhibition of REV3 expression in cancer cells leads to an accumulation of persistent DNA damage as indicated by an increase in phospho-ATM, 53BP1, and phospho-H2AX foci formation, subsequently leading to the activation of the ATM-dependent DNA damage response cascade. REV3 depletion in p53-proficient cancer cell lines results in a G1 arrest and induction of senescence as indicated by the accumulation of p21 and an increase in senescence-associated β-galactosidase activity. In contrast, inhibition of REV3 expression in p53-deficient cells results in growth inhibition and a G2/M arrest. A small fraction of the p53-deficient cancer cells can overcome the G2/M arrest, which results in mitotic slippage and aneuploidy. Our findings reveal that REV3 depletion per se suppresses growth of cancer cell lines from different origin, whereas control cell lines and a mesothelial primary culture were less affected. Thus, our findings indicate that depletion of REV3 not only can amend cisplatin-based cancer therapy but also can be applied for susceptible cancers as a potential monotherapy. PMID:22028621

  15. Pharmacological Targeting the REV-ERBs in Sleep/Wake Regulation

    PubMed Central

    Amador, Ariadna; Huitron-Resendiz, Salvador; Roberts, Amanda J.; Kamenecka, Theodore M.; Solt, Laura A.; Burris, Thomas P.

    2016-01-01

    The circadian clock maintains appropriate timing for a wide range of behaviors and physiological processes. Circadian behaviors such as sleep and wakefulness are intrinsically dependent on the precise oscillation of the endogenous molecular machinery that regulates the circadian clock. The identical core clock machinery regulates myriad endocrine and metabolic functions providing a link between sleep and metabolic health. The REV-ERBs (REV-ERBα and REV-ERBβ) are nuclear receptors that are key regulators of the molecular clock and have been successfully targeted using small molecule ligands. Recent studies in mice suggest that REV-ERB-specific synthetic agonists modulate metabolic activity as well as alter sleep architecture, inducing wakefulness during the light period. Therefore, these small molecules represent unique tools to extensively study REV-ERB regulation of sleep and wakefulness. In these studies, our aim was to further investigate the therapeutic potential of targeting the REV-ERBs for regulation of sleep by characterizing efficacy, and optimal dosing time of the REV-ERB agonist SR9009 using electroencephalographic (EEG) recordings. Applying different experimental paradigms in mice, our studies establish that SR9009 does not lose efficacy when administered more than once a day, nor does tolerance develop when administered once a day over a three-day dosing regimen. Moreover, through use of a time response paradigm, we determined that although there is an optimal time for administration of SR9009 in terms of maximal efficacy, there is a 12-hour window in which SR9009 elicited a response. Our studies indicate that the REV-ERBs are potential therapeutic targets for treating sleep problems as those encountered as a consequence of shift work or jet lag. PMID:27603791

  16. Pharmacological Targeting the REV-ERBs in Sleep/Wake Regulation.

    PubMed

    Amador, Ariadna; Huitron-Resendiz, Salvador; Roberts, Amanda J; Kamenecka, Theodore M; Solt, Laura A; Burris, Thomas P

    2016-01-01

    The circadian clock maintains appropriate timing for a wide range of behaviors and physiological processes. Circadian behaviors such as sleep and wakefulness are intrinsically dependent on the precise oscillation of the endogenous molecular machinery that regulates the circadian clock. The identical core clock machinery regulates myriad endocrine and metabolic functions providing a link between sleep and metabolic health. The REV-ERBs (REV-ERBα and REV-ERBβ) are nuclear receptors that are key regulators of the molecular clock and have been successfully targeted using small molecule ligands. Recent studies in mice suggest that REV-ERB-specific synthetic agonists modulate metabolic activity as well as alter sleep architecture, inducing wakefulness during the light period. Therefore, these small molecules represent unique tools to extensively study REV-ERB regulation of sleep and wakefulness. In these studies, our aim was to further investigate the therapeutic potential of targeting the REV-ERBs for regulation of sleep by characterizing efficacy, and optimal dosing time of the REV-ERB agonist SR9009 using electroencephalographic (EEG) recordings. Applying different experimental paradigms in mice, our studies establish that SR9009 does not lose efficacy when administered more than once a day, nor does tolerance develop when administered once a day over a three-day dosing regimen. Moreover, through use of a time response paradigm, we determined that although there is an optimal time for administration of SR9009 in terms of maximal efficacy, there is a 12-hour window in which SR9009 elicited a response. Our studies indicate that the REV-ERBs are potential therapeutic targets for treating sleep problems as those encountered as a consequence of shift work or jet lag.

  17. Rev-Erbs repress macrophage gene expression by inhibiting enhancer-directed transcription

    PubMed Central

    Lam, Michael T.Y.; Cho, Han; Lesch, Hanna P.; Gosselin, David; Heinz, Sven; Tanaka-Oishi, Yumiko; Benner, Christopher; Kaikkonen, Minna U.; Kim, Aneeza S.; Kosaka, Mika; Lee, Cindy Y.; Watt, Andy; Grossman, Tamar R.; Rosenfeld, Michael G.; Evans, Ronald M.; Glass, Christopher K.

    2013-01-01

    Rev-Erbα and Rev-Erbβ are nuclear receptors that regulate the expression of genes involved in the control of circadian rhythm1,2, metabolism3,4, and inflammatory responses5. Rev-Erbs function as transcriptional repressors by recruiting NCoR/HDAC3 co-repressor complexes to Rev-Erb response elements in enhancers and promoters of target genes6-8, but the molecular basis for cell-specific programs of repression is not known. Here, we present evidence that in macrophages, Rev-Erbs regulate target gene expression by inhibiting the functions of distal enhancers that are selected by macrophage lineage-determining factors, thereby establishing a macrophage-specific program of repression. Remarkably, the repressive functions of Rev-Erbs are associated with their ability to inhibit the transcription of enhancer-derived RNAs (eRNAs). Furthermore, targeted degradation of eRNAs at two enhancers subject to negative regulation by Rev-Erbs resulted in reduced expression of nearby mRNAs, implying a direct role of these eRNAs in enhancer function. By precisely defining eRNA start sites using a method that quantifies nascent 5′ ends (5′-GRO-Seq), we show that transfer of full enhancer activity to a target promoter requires both the sequences mediating transcription factor binding and the specific sequences encoding the eRNA transcript. These studies provide evidence for direct roles of eRNAs in contributing to enhancer functions and suggest that Rev-Erbs act to suppress gene expression at a distance by repressing eRNA transcription. PMID:23728303

  18. Solid Micro Horn Array (SMIHA) for Acoustic Matching

    NASA Technical Reports Server (NTRS)

    Sherrit, S.; Bao, X.; Bar-Cohen, Y.

    2008-01-01

    Transduction of electrical signals to mechanical signals and vice-versa in piezoelectric materials is controlled by the material coupling coefficient. In general in a loss-less material the ratio of energy conversion per cycle is proportional to the square of the coupling coefficient. In practical transduction however the impedance mismatch between the piezoelectric material and the electrical drive circuitry or the mechanical structure can have a significant impact on the power transfer. This paper looks at novel methods of matching the acoustic impedance of structures to the piezoelectric material in an effort to increase power transmission and efficiency. In typical methods the density and acoustic velocity of the matching layer is adjusted to give good matching between the transducer and the load. The approach discussed in this paper utilizes solid micro horn arrays in the matching layer which channel the stress and increase the strain in the layer. This approach is found to have potential applications in energy harvesting, medical ultrasound and in liquid and gas coupled transducers.

  19. Polarization entanglement purification for concatenated Greenberger-Horne-Zeilinger state

    NASA Astrophysics Data System (ADS)

    Zhou, Lan; Sheng, Yu-Bo

    2017-10-01

    Entanglement purification plays a fundamental role in long-distance quantum communication. In the paper, we put forward the first polarization entanglement purification protocol (EPP) for one type of nonlocal logic-qubit entanglement, i.e., concatenated Greenberger-Horne-Zeilinger (C-GHZ) state, resorting to the photon-atom interaction in low-quality (Q) cavity. In contrast to existing EPPs, this protocol can purify the bit-flip error and phase-flip error in both physic and logic level. Instead of measuring the photons directly, this protocol only requires to measure the atom states to judge whether the protocol is successful. In this way, the purified logic entangled states can be preserved for further application. Moreover, it makes this EPP repeatable so as to obtain a higher fidelity of logic entangled states. As the logic-qubit entanglement utilizes the quantum error correction (QEC) codes, which has an inherent stability against noise and decoherence, this EPP combined with the QEC codes may provide a double protection for the entanglement from the channel noise and may have potential applications in long-distance quantum communication.

  20. Presumptive keratoglobus in a great horned owl (Bubo virginianus).

    PubMed

    Lau, Rachael K; Moresco, Anneke; Woods, Sarah J; Reilly, Christopher M; Hawkins, Michelle G; Murphy, Christopher J; Hollingsworth, Steven R; Hacker, Dennis; Freeman, Kate S

    2016-07-31

    A juvenile to young adult, male, great horned owl (Bubo virginianus,GHOW) was presented to the wildlife rehabilitation hospital at Lindsay Wildlife Museum (WRHLWM) due to trauma to the right patagium from barbed wire entanglement. On presentation, both corneas were irregular, dry, and no movement of the third eyelid was noted. A severe corneal enlargement/globoid appearance was the predominant ophthalmic feature. The fundus was normal in both eyes (OU). Over the course of several days, both corneas developed edema combined with further dessication at the ocular surface associated with diffuse dorsal fluorescein stain uptake. Repeated ophthalmic examinations found normal intraocular pressures and an inability to move the third eyelid over the enlarged corneas. The bird was deemed nonreleasable due to severe wing damage and poor prognosis associated with eye abnormalities and was humanely euthanized. Postmortem CT, enucleation, and histopathology were performed to evaluate the ocular anatomical abnormality and confirm the suspected diagnosis of keratoglobus. This GHOW represents the first reported case of presumptive keratoglobus in a raptor. © 2016 American College of Veterinary Ophthalmologists.