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Sample records for host present-day experimental

  1. Specific allogeneic unresponsiveness in the adult host: present-day experimental models

    SciTech Connect

    Rapaport, F.T.; Bachvaroff, R.J.; Cronkite, E.; Chanana, A.; Sato, T.; Asari, H.; Waltzer, W.C.

    1982-01-01

    As part of a long-term intensive effort to apply the induction of adult allogensic unresponsiveness to the transplantation problem, two techniques to control the variability in the persistence of immunologically competent postthymic cells iin the treated host and/or the inoculum of autologous marrow returned to the host after irradiation are described. The first consisted of exposing the peripheral blood of prospective recipients to a 5-week course of extra-corporeal irradiation (ECIB), the other of exposing the stored autologous marrow scheduled to repopulate a given recipient to methyl-prednisolone (MPd) and DNase prior to renifusion into the recipient. Serial analysis of bone marrow cell samples at various intervals before and after treatment was undertaken. The significance of the disappearance of a particular population of nonnuclear cells from the samples, and the association of such disappearance with increased success in the induction of allogeneic unresponsiveness is discussed. (ACR)

  2. Lexical Change in Present-Day English.

    ERIC Educational Resources Information Center

    Baron, Dennis E.

    The lexicon of present-day English is changing rapidly and regularly, and a description and explanation of this change is necessary for any comprehensive diachronic theory. An examination of a corpus of 500 new words collected during 1975 provides the basis for a typology of lexical change that both supports and suggests modifications for the…

  3. Stability of liquid saline water on present day Mars

    NASA Astrophysics Data System (ADS)

    Zorzano, M.-P.; Mateo-Martí, E.; Prieto-Ballesteros, O.; Osuna, S.; Renno, N.

    2009-10-01

    Perchlorate salts (mostly magnesium and sodium perchlorate) have been detected on Mars' arctic soil by the Phoenix lander, furthermore chloride salts have been found on the Meridiani and Gusev sites and on widespread deposits on the southern Martian hemisphere. The presence of these salts on the surface is not only relevant because of their ability to lower the freezing point of water, but also because they can absorb water vapor and form a liquid solution (deliquesce). We show experimentally that small amounts of sodium perchlorate (˜ 1 mg), at Mars atmospheric conditions, spontaneously absorb moisture and melt into a liquid solution growing into ˜ 1 mm liquid spheroids at temperatures as low as 225 K. Also mixtures of water ice and sodium perchlorate melt into a liquid at this temperature. Our results indicate that salty environments make liquid water to be locally and sporadically stable on present day Mars.

  4. Common Characteristics of Models in Present-Day Scientific Practice

    ERIC Educational Resources Information Center

    Van Der Valk, Ton; Van Driel, Jan H.; De Vos, Wobbe

    2007-01-01

    Teaching the use of models in scientific research requires a description, in general terms, of how scientists actually use models in their research activities. This paper aims to arrive at defining common characteristics of models that are used in present-day scientific research. Initially, a list of common features of models and modelling, based…

  5. Upstream Material Accumulation and Meandering on Present Day Gully Evolution

    NASA Astrophysics Data System (ADS)

    Pasquon, K.; Gargani, J.; Massé, M.; Conway, S. J.; Vincendon, M.; Séjourné, A.

    2016-09-01

    Here we show the present day evolution of a martian gully during the last 4 MY: (1) non-seasonal material accumulation in the alcove, (2) seasonal evolution of meanders, (3) extension of the channel, (4) significant modifications of the debris apron.

  6. WORD-MAKING IN PRESENT-DAY ENGLISH.

    ERIC Educational Resources Information Center

    SIMONINI, R.C., JR.

    WORDS CAN BE STUDIED BY DESCRIBING THEIR ORIGIN INDUCTIVELY OR DEDUCTIVELY. EITHER WAY, A PRECISE DEFINITION OF ETYMOLOGICAL CLASSES WHICH ARE MUTUALLY EXCLUSIVE IS NEEDED. PRESENT-DAY ENGLISH IS CLASSIFIED INTO--(1) NATIVE WORDS WHICH CAN BE TRACED BACK TO THE WORD STOCK OF OLD ENGLISH, (2) LOAN WORDS NEW TO THE ENGLISH LANGUAGE WHICH HAD…

  7. A new present-day velocity field for eastern Iran

    NASA Astrophysics Data System (ADS)

    Walpersdorf, A.; Tavakoli, F.; Hatzfeld, D.; Jadidi, A.; Vergnolle, M.; Djamour, Y.; Nankali, H. R.; Sedighi, M.; Bellier, O.; Shabanian, E.

    2009-04-01

    Since 2004, extensive GPS campaigns and the upcoming Iranian permanent GPS network are monitoring the present-day deformation in eastern Iran. We present a new GPS velocity field that extends from Central Iran to the Turkmen shield and the Hellmand block on the Eurasian plate. It permits to monitor the right lateral shear across the aseismic Lut block between Central Iran and the Hellmand block, and the resulting shortening across the Kopeh Dagh mountain belt limiting NE Iran towards Turkmenistan. The present-day deformation pattern is used to verify existing tectonic models. Individual instantaneous fault slip rates are compared to short term and long term geological estimates. We find that GPS slip rates are generally coherent with short term geologic determinations (from dating of geomorphologic offsets over some 10-100 ka). Some differences with respect to long term estimates (from total geologic fault offsets and onset ages of several Ma) indicate non-constant slip rates over different time scales or that the onset of the present-day deformation presumed to 3-7 Ma in eastern Iran has to be revised.

  8. Overcoming Present-Day Powerplant Limitations Via Unconventional Engine Configurations

    NASA Technical Reports Server (NTRS)

    Meitner, Peter L.

    2006-01-01

    The Army Research Laboratory s Vehicle Technology Directorate is sponsoring the prototype development of three unconventional engine concepts - two intermittent combustion (IC) engines and one turbine engine (via SBIR (Small Business Innovative Research) contracts). The IC concepts are the Nutating Engine and the Bonner Engine, and the turbine concept is the POWER Engine. Each of the three engines offers unique and greatly improved capabilities (which cannot be achieved by present-day powerplants), while offering significant reductions in size and weight. This paper presents brief descriptions of the physical characteristics of the three engines, and discusses their performance potentials, as well as their development status.

  9. Deep history impacts present-day ecology and biodiversity.

    PubMed

    Vitt, Laurie J; Pianka, Eric R

    2005-05-31

    Lizards and snakes putatively arose between the early Jurassic and late Triassic; they diversified worldwide and now occupy many different ecological niches, making them ideal for testing theories on the origin of ecological traits. We propose and test the "deep history hypothesis," which claims that differences in ecological traits among species arose early in evolutionary history of major clades, and that present-day assemblages are structured largely because of ancient, preexisting differences. We combine phylogenetic data with ecological data collected over nearly 40 years to reconstruct the evolution of dietary shifts in squamate reptiles. Data on diets of 184 lizard species in 12 families from 4 continents reveal significant dietary shifts at 6 major divergence points, reducing variation by 79.8%. The most striking dietary divergence (27.6%) occurred in the late Triassic, when Iguania and Scleroglossa split. These two clades occupy different regions of dietary niche space. Acquisition of chemical prey discrimination, jaw prehension, and wide foraging provided scleroglossans access to sedentary and hidden prey that are unavailable to iguanians. This cladogenic event may have profoundly influenced subsequent evolutionary history and diversification. We suggest the hypothesis that ancient events in squamate cladogenesis, rather than present-day competition, caused dietary shifts in major clades such that some lizard clades gained access to new resources, which in turn led to much of the biodiversity observed today.

  10. Present-day dynamic and residual topography in central Anatolia

    NASA Astrophysics Data System (ADS)

    Uluocak, Ebru Şengül; Pysklywec, Russell; Göğüş, Oğuz H.

    2016-06-01

    The Central Anatolian orogenic plateau is represented by young volcanism, rapid plateau uplift, and distinctive (past and active) tectonic deformation. In this study, we consider observational data in terms of regional present-day geodynamics in the region. The residual topography of Central Anatolia was derived to define the regional isostatic conditions according to Airy isostasy and infer the potential role of "dynamic topography". Two-dimensional thermo-mechanical forward models for coupled mantle-lithosphere flow/deformation were conducted along a N-S directional profile through the region (e.g. northern/Pontides, interior, and southern/Taurides). These models were based on seismic tomography data that provide estimates about the present-day mantle thermal structure beneath the Anatolian plate. We compare the modelling results with calculated residual topography and independent data sets of geological deformation, gravity, and high surface heat flow/widespread geothermal activity. Model results suggest that there is ˜1 km of mantle flow induced dynamic topography associated with the sub-lithospheric flow driven by the seismically-inferred mantle structure. The uprising mantle may have also driven the asthenospheric source of volcanism in the north (e.g. Galatia volcanic province) and the Cappadocia volcanic province in the south while elevating the surface in the last 10 Myrs. Our dynamic topography calculations emphasize the role of vertical forcing under other orogenic plateaux underlain by relatively thin crust and low-density asthenospheric mantle.

  11. Present-day kinematics of Adria and its southern boundary

    NASA Astrophysics Data System (ADS)

    D'Agostino, N.; Selvaggi, G.; Avallone, A.; Cheloni, D.; D'Anastasio, E.; Mantenuto, S.

    2006-12-01

    In this presentation I will review the observations that support an indipendent kinematics of the Adriatic region with respect to the Eurasia and Nubia plates. CGPS and earthquake slip vectors suggest that Adria can be divided in two independent blocks, a northern and a southern sector, whose limit can be placed across the Gargano seismic region. The Northern Adria block is currently rotating counterclockwise at ~0.2°/myr with respect to Eurasia around a pole of rotation located in the western part of the Po Plain. GPS sites in Apulia show that this region is rotating clockwise to Adria around a pole of rotation located in the middle Adriatic. The discrepanciy between the present-day kinematics and crustal deformation pattern that has dominated over Neogene and Quaternary times (convergence in the Alps and retreating subduction in the Apennines) will be discussed. By a mechanical point of view the spatial correspondence of the block boundaries with their relative poles of rotation suggest that the kinematics is controlled by the interactions between the blocks previously forming the Adriatic African promontory. These findings has important implications for the kinematics of the Ionian region and the present-day activity of the Calabrian subduction.

  12. Present-day dynamic and residual topography in Central Anatolia

    NASA Astrophysics Data System (ADS)

    Şengül Uluocak, Ebru; Pysklywec, Russell; Göǧüş, Oǧuz H.

    2016-09-01

    The Central Anatolian orogenic plateau is represented by young volcanism, rapid plateau uplift and distinctive (past and active) tectonic deformation. In this study, we consider observational data in terms of regional present-day geodynamics in the region. The residual topography of Central Anatolia was derived to define the regional isostatic conditions according to Airy isostasy and infer the potential role of `dynamic topography'. 2-D thermomechanical forward models for coupled mantle-lithosphere flow/deformation were conducted along an N-S directional profile through the region (e.g. northern/Pontides, interior and southern/Taurides). These models were based on seismic tomography data that provide estimates about the present-day mantle thermal structure beneath the Anatolian plate. We compare the modelling results with calculated residual topography and independent data sets of geological deformation, gravity and high surface heat flow/widespread geothermal activity. Model results suggest that there is ˜1 km of mantle flow induced dynamic topography associated with the sublithospheric flow driven by the seismically inferred mantle structure. The uprising mantle may have also driven the asthenospheric source of volcanism in the north (e.g. Galatia volcanic province) and the Cappadocia volcanic province in the south while elevating the surface in the last 10 Myr. Our dynamic topography calculations emphasize the role of vertical forcing under other orogenic plateaux underlain by relatively thin crust and low-density asthenospheric mantle.

  13. The delayed rise of present-day mammals.

    PubMed

    Bininda-Emonds, Olaf R P; Cardillo, Marcel; Jones, Kate E; MacPhee, Ross D E; Beck, Robin M D; Grenyer, Richard; Price, Samantha A; Vos, Rutger A; Gittleman, John L; Purvis, Andy

    2007-03-29

    Did the end-Cretaceous mass extinction event, by eliminating non-avian dinosaurs and most of the existing fauna, trigger the evolutionary radiation of present-day mammals? Here we construct, date and analyse a species-level phylogeny of nearly all extant Mammalia to bring a new perspective to this question. Our analyses of how extant lineages accumulated through time show that net per-lineage diversification rates barely changed across the Cretaceous/Tertiary boundary. Instead, these rates spiked significantly with the origins of the currently recognized placental superorders and orders approximately 93 million years ago, before falling and remaining low until accelerating again throughout the Eocene and Oligocene epochs. Our results show that the phylogenetic 'fuses' leading to the explosion of extant placental orders are not only very much longer than suspected previously, but also challenge the hypothesis that the end-Cretaceous mass extinction event had a major, direct influence on the diversification of today's mammals.

  14. Network archaeology: uncovering ancient networks from present-day interactions.

    PubMed

    Navlakha, Saket; Kingsford, Carl

    2011-04-01

    What proteins interacted in a long-extinct ancestor of yeast? How have different members of a protein complex assembled together over time? Our ability to answer such questions has been limited by the unavailability of ancestral protein-protein interaction (PPI) networks. To overcome this limitation, we propose several novel algorithms to reconstruct the growth history of a present-day network. Our likelihood-based method finds a probable previous state of the graph by applying an assumed growth model backwards in time. This approach retains node identities so that the history of individual nodes can be tracked. Using this methodology, we estimate protein ages in the yeast PPI network that are in good agreement with sequence-based estimates of age and with structural features of protein complexes. Further, by comparing the quality of the inferred histories for several different growth models (duplication-mutation with complementarity, forest fire, and preferential attachment), we provide additional evidence that a duplication-based model captures many features of PPI network growth better than models designed to mimic social network growth. From the reconstructed history, we model the arrival time of extant and ancestral interactions and predict that complexes have significantly re-wired over time and that new edges tend to form within existing complexes. We also hypothesize a distribution of per-protein duplication rates, track the change of the network's clustering coefficient, and predict paralogous relationships between extant proteins that are likely to be complementary to the relationships inferred using sequence alone. Finally, we infer plausible parameters for the model, thereby predicting the relative probability of various evolutionary events. The success of these algorithms indicates that parts of the history of the yeast PPI are encoded in its present-day form. PMID:21533211

  15. ADAPTION OF NONSTANDARD PIPING COMPONENTS INTO PRESENT DAY SEISMIC CODES

    SciTech Connect

    D. T. Clark; M. J. Russell; R. E. Spears; S. R. Jensen

    2009-07-01

    With spiraling energy demand and flat energy supply, there is a need to extend the life of older nuclear reactors. This sometimes requires that existing systems be evaluated to present day seismic codes. Older reactors built in the 1960s and early 1970s often used fabricated piping components that were code compliant during their initial construction time period, but are outside the standard parameters of present-day piping codes. There are several approaches available to the analyst in evaluating these non-standard components to modern codes. The simplest approach is to use the flexibility factors and stress indices for similar standard components with the assumption that the non-standard component’s flexibility factors and stress indices will be very similar. This approach can require significant engineering judgment. A more rational approach available in Section III of the ASME Boiler and Pressure Vessel Code, which is the subject of this paper, involves calculation of flexibility factors using finite element analysis of the non-standard component. Such analysis allows modeling of geometric and material nonlinearities. Flexibility factors based on these analyses are sensitive to the load magnitudes used in their calculation, load magnitudes that need to be consistent with those produced by the linear system analyses where the flexibility factors are applied. This can lead to iteration, since the magnitude of the loads produced by the linear system analysis depend on the magnitude of the flexibility factors. After the loading applied to the nonstandard component finite element model has been matched to loads produced by the associated linear system model, the component finite element model can then be used to evaluate the performance of the component under the loads with the nonlinear analysis provisions of the Code, should the load levels lead to calculated stresses in excess of Allowable stresses. This paper details the application of component-level finite

  16. On the Stability of Liquid Water on Present Day Mars

    NASA Technical Reports Server (NTRS)

    Haberle, Robert M.; DeVincenzi, Donald L. (Technical Monitor)

    2000-01-01

    The mean annual surface pressure and temperature on present day Mars do not allow for the long term stability of liquid water on the surface. However, theoretical arguments have been advanced that suggest liquid water could form in transient events even though it would not be in equilibrium with the environment. Using a Mars General Circulation Model, we calculate where and for how long the surface pressure and surface temperature meet the minimum requirements for this metastability of liquid water. These requirements are that the pressure and temperature must be above the triple point of water, but below its boiling point. We find that there are five regions on Mars where these requirements are periodically satisfied: in the near equatorial regions of Amazonis, Arabia, and Elysium, and in the Hellas and Argyre impact basins. Whether liquid water ever forms in these regions depends on the availability of ice and heat, and on the evaporation rate. The latter is poorly understood for low pressure CO2 environments, but is likely to be so high that melting occurs rarely, if at all. However, in the relatively recent past, surface pressures may have been higher than they are today perhaps by as much as a factor of 2 or 3. Under these circumstances melting would have been easier to achieve. We plan to undertake laboratory experiments to better understand the potential for melting in low pressure environments.

  17. The mutating preconscious archetype in present-day ecological conditions.

    PubMed

    Włoch, K

    1990-12-01

    Competition is an important force behind evolution in present-day ecological conditions, its intensity varying according to the organisms' expectations vis-à-vis resources available. On the other hand, the role of mutation in the evolutionary process can hardly be underestimated: Leading to the change of the preconsciously functioning archetype, mutation makes it impossible for the ego to realize its image. This is a consequence of the clash between the mutated, preconsciously functioning archetype and the system of ethical and moral norms functioning in the collective superego of a given population group. The type of mutation that results from the accelerated pace of evolution can be viewed as a continuity in the development of an organism's behavior. The preconsciously functioning archetype, resulting from these mutations, contains impulses and predispositions that differ markedly from the impulses and predispositions functioning in the genetically determined "pattern of behavior." In a situation where anxiety results from the inhibition of impulses and dispositions, hostility reactions are likely to occur. In its turn, reactive hostility can induce anxiety, thus creating a reaction cycle. Underlying this mechanism is the mutated, preconsciously functioning archetype, which itself is a result of the development of civilization in our contemporary world.

  18. Present-day kinematics of the East African Rift

    NASA Astrophysics Data System (ADS)

    Saria, E.; Calais, E.; Stamps, D. S.; Delvaux, D.; Hartnady, C. J. H.

    2014-04-01

    The East African Rift (EAR) is a type locale for investigating the processes that drive continental rifting and breakup. The current kinematics of this ~5000 km long divergent plate boundary between the Nubia and Somalia plates is starting to be unraveled thanks to a recent augmentation of space geodetic data in Africa. Here we use a new data set combining episodic GPS measurements with continuous measurements on the Nubian, Somalian, and Antarctic plates, together with earthquake slip vector directions and geologic indicators along the Southwest Indian Ridge to update the present-day kinematics of the EAR. We use geological and seismological data to determine the main rift faults and solve for rigid block rotations while accounting for elastic strain accumulation on locked active faults. We find that the data are best fit with a model that includes three microplates embedded within the EAR, between Nubia and Somalia (Victoria, Rovuma, and Lwandle), consistent with previous findings but with slower extension rates. We find that earthquake slip vectors provide information that is consistent with the GPS velocities and helps to significantly reduce uncertainties of plate angular velocity estimates. We also find that 3.16 Myr MORVEL average spreading rates along the Southwest Indian Ridge are systematically faster than prediction from GPS data alone. This likely indicates that outward displacement along the SWIR is larger than the default value used in the MORVEL plate motion model.

  19. Present-day microplates motion in the central Mediterranean

    NASA Astrophysics Data System (ADS)

    D'Agostino, N.

    2007-12-01

    Motion of microplates in diffuse plate boundaries often accommodates convergence between major plates. In this work we present a kinematic model for the central mediterranean sector of the Eurasia-Nubia plate boundary, derived from GPS and earthquake slip vectors, which describe the crustal motion as resulting from the interactions of two rigid microplates: Adria and Apulia-Ionia. The styles of deformation predicted by the kinematic model along the boundaries of the proposed microplates are consistent with geological observations and present significant implications for the characterization of seismically active regions in the Eurasia-Nubia plate boundary. Microplates rotation rates are succesfully reproduced by a simple slat model in which the edges of the microplates are constrained to move with the adjacent plate or microplate, suggesting a significant coupling between crustal blocks and the primary role of edge-transmitted forces as driving factor for crustal motion. These results are compared with surface wave tomographic models and other seismological observables which suggest that the rigid microplates correspond to regions of high shear wave velocities in the upper mantle and high integrated lithospheric strength. The present-day plate boundary configuration derives from the inherited Mesozoic African margin, from the rapid Neogene evolution of the Tyrrhenian subduction zone and the final fragmentation of the Adriatic promontory.

  20. Present-day sea level rise: A synthesis

    NASA Astrophysics Data System (ADS)

    Cazenave, Anny; Lombard, Alix; Llovel, William

    2008-11-01

    Measuring sea level change and understanding its causes have improved considerably in the recent years, essentially because new in situ and remote sensing data sets have become available. Here we report on the current knowledge of present-day sea level change. We briefly present observational results on sea level change from satellite altimetry since 1993 and tide gauges for the past century. We next discuss recent progress made in quantifying the processes causing sea level change on time scales ranging from years to decades, i.e., thermal expansion, land ice mass loss and land water storage change. For the 1993-2003 decade, the sum of climate-related contributions agree well (within the error bars) with the altimetry-based sea level, half of the observed rate of rise being due to ocean thermal expansion, land ice plus land waters explaining the other half. Since about 2003, thermal expansion increase has stopped, whereas the sea level continues to rise, although at a reduced rate compared to the previous decade (2.5 mm/yr versus 3.1 mm/yr). Recent increases in glacier melting and ice mass loss from the ice sheets appear able to account alone for the rise in sea level reported over the last five years.

  1. [Present day radiation diagnosis of renal tumors and cysts].

    PubMed

    Vlasov, P V; Kotliarov, P M

    1997-01-01

    The paper discusses the present-day radiation diagnosis of renal bulky formations: tumors and cysts. Based on the study of a great body of clinical data on 708 patients, the authors showed the potentialities of routine X-ray and currently available radiation techniques in the diagnosis of benign and malignant tumors and cysts of the kidney. Based on the data available in the literature and their own findings, the authors concluded that at the earliest stages of their preclinical development, tumors can be today diagnosed by a complete package of up-to-date diagnostic tools. Ultrasonic and computerized tomographies are of the highest informative value in diagnosing tumors and cystic formations. However, in some cases, a diagnosis is made via a routine excretory urography, angiography and needle biopsy. Excretory urography is of limited value for the diagnosis of renal bulky formations and it is used as an auxiliary mainly when a pathological process takes place in the renal hilus area. Angiography retains its significance as a supplementary technique. In the vast majority of cases, an accurate nosological diagnosis can be made on the basis of evaluation of the pattern of blood supply (the presence of abnormal vessels and their topographic features). The paper details the semiotics of a wide range of renal bulky processes by applying all currently available techniques of radiation diagnosis.

  2. The present-day number of tectonic plates

    NASA Astrophysics Data System (ADS)

    Harrison, Christopher G. A.

    2016-03-01

    The number of tectonic plates on Earth described in the literature has expanded greatly since the start of the plate tectonic era, when only about a dozen plates were considered in global models of present-day plate motions. With new techniques of more accurate earthquake epicenter locations, modern ways of measuring ocean bathymetry using swath mapping, and the use of space based geodetic techniques, there has been a huge growth in the number of plates thought to exist. The study by Bird (2003) proposed 52 plates, many of which were delineated on the basis of earthquake locations. Because of the pattern of areas of these plates, he suggested that there should be more small plates than he could identify. In this paper, I gather together publications that have proposed a total of 107 new plates, giving 159 plates in all. The largest plate (Pacific) is about 20 % of the Earth's area or 104 Mm2, and the smallest of which (Plate number 5 from Hammond et al. 2011) is only 273 km2 in area. Sorting the plates by size allows us to investigate how size varies as a function of order. There are several changes of slope in the plots of plate number organized by size against plate size order which are discussed. The sizes of the largest seven plates is constrained by the area of the Earth. A middle set of 73 plates down to an area of 97,563 km2 (the Danakil plate at number 80, is the plate of median size) follows a fairly regular pattern of plate size as a function of plate number. For smaller plates, there is a break in the slope of the plate size/plate number plot and the next 32 plates follow a pattern of plate size proposed by the models of Koehn et al. (2008) down to an area of 11,638 km2 (West Mojave plate # 112). Smaller plates do not follow any regular pattern of area as a function of plate number, probably because we have not sampled enough of these very small plates to reveal any clear pattern.

  3. Nitrogen evolution and present day distribution on Mars

    NASA Astrophysics Data System (ADS)

    Banin, A.; Mancinelli, R. L.

    2003-04-01

    Nitrogen is an essential element for life. Specifically, fixed nitrogen (i.e., NH_3, NH_4^+, NO_3^-, NO_2^- and N chemically bound to either inorganic or organic molecules and is releasable by hydrolysis to NH_3 or NH_4^+) is the form of nitrogen useful to living organisms. Nitrogen on present-day Mars has been analyzed only in the atmosphere. The inventory is a small fraction of the nitrogen complement presumed to have been received by the planet during its accretion. Where is the missing N? Answering this question is crucial for understanding of the probability of life evolution on Mars and for future exobiological exploration of this intriguing planet. Two main processes could have removed N from the atmosphere: 1) escape to space; 2) burial within the regolith. Non thermal escape to space due to atmospheric erosion has been suggested but its extent has not been constrained yet. No traces of organic compounds were detected in Mars soil by the Viking Landers. However, direct in situ analysis of mineral N concentration in Martian soils and rocks has not been performed yet. Due to the lack of neither biological (denitrification) nor geological (plate tectonics) recycling of N on the surface of Mars, nitrogen may have been stored in the Martian regolith as soluble inorganic salts of NO_3^- and NH_4^+, and as mineral-bound NH_4^+. Nitrates will be stable in the highly oxidized surface soil of Mars, and will tend to accumulate there. Such accumulations are observed in cold and extremely arid environments on Earth (e.g. Antarctica, the Atacama Desert). NH_4^+-N may be bound and stabilized in the soil replacing K as a structural cation in silicate minerals. In this paper we constrain the possible total N content in the Mars crust/regolith using information obtained from Mars (SNC) meteorites analyses. Further, we briefly discuss chemical, physical and, possibly, biological processes that may have affected the patterns of N distribution in the top horizons of Mars

  4. Present-day climatic equivalents of European Cenozoic climates

    NASA Astrophysics Data System (ADS)

    Utescher, Torsten; Mosbrugger, Volker; Ivanov, Dimiter; Dilcher, David L.

    2009-07-01

    Recently, continental climate evolution in Central Europe over the last 45 Ma has been reconstructed from the palaeobotanical record using a Nearest Living Relative methodology (Coexistence Approach; CA). The reconstructed climate curves document in detail the transition from almost tropical conditions in the Mid-Eocene to a temperate climate at the Pliocene/Pleistocene transition. The observed climatic shifts are primarily expressed as non-proportional changes of the different variables taken into account. In the present study a published palaeoclimate data set for a total of 42 macrofloras complemented by new calculations is used as base to analyse the climatic space in which a fossil flora existed. To define these spaces CA intervals calculated for 3 temperature (mean annual temperature, cold and warm month mean) and 3 precipitation variables (mean annual precipitation, mean monthly precipitation of the driest and of the wettest month) are combined. Using a global gridded climatology (10' resolution), this climate space is then utilized to identify Recent climate analogues with respect to the variables regarded. For 18 macrofloras climatic analogue regions with respect to 6 variables are identified on the globe. For 16 macrofloras, analogues exist when three temperature parameters and mean annual precipitation are regarded. No Recent equivalents are found in 8 cases. This corroborates the assumption of the temporary existence of non-analogue climates in the Cenozoic. As shown by multivariate statistics the observed anomalies with respect to present-day conditions basically refer to high winter temperatures. Deploying a GIS, the Recent climate analogues can be presented as sets of grid cells for each flora that can be mapped on a globe. Once identified, these regions can be merged with adequate thematic layers to assess additional proxy data for the palaeofloras. To exemplify the procedure Koeppen climate type, numbers of days with ground frost, as well as

  5. Accounting for reciprocal host-microbiome interactions in experimental science.

    PubMed

    Stappenbeck, Thaddeus S; Virgin, Herbert W

    2016-06-08

    Mammals are defined by their metagenome, a combination of host and microbiome genes. This knowledge presents opportunities to further basic biology with translation to human diseases. However, the now-documented influence of the metagenome on experimental results and the reproducibility of in vivo mammalian models present new challenges. Here we provide the scientific basis for calling on all investigators, editors and funding agencies to embrace changes that will enhance reproducible and interpretable experiments by accounting for metagenomic effects. Implementation of new reporting and experimental design principles will improve experimental work, speed discovery and translation, and properly use substantial investments in biomedical research.

  6. Present Day Activity of South Polar Gullies on Mars

    NASA Astrophysics Data System (ADS)

    Raack, J.; Reiss, D.; Ruesch, O.; Hiesinger, H.

    2012-04-01

    Here we report on clearly identified seasonal changes of gullies observed within the last two martian years (MY) on slopes of a south polar pit, which is located in a filled crater (diameter ~54 km) north of Sisyphi Cavi at ~68.5°S and ~1.5°E. Using new high-resolution imaging (High Resolution Imaging Science Experiment, HiRISE), temperature (Thermal Emission Spectrometer, TES) and spectral data (Compact Reconnaissance Imaging Spectrometer for Mars, CRISM; Observatoire pour la Minéralogie, l'Eau, les Glaces et l'Activité, OMEGA), we analyzed the exact timing of changes of gullies and detect the possible medium (CO2, H2O or dry) and mechanism which initiate present day gully activity. Two locations in the study region with clear modifications of gullies were identified in MY 29 between LS 226° and LS 247° and between LS 209° and LS 247°. In MY 30 changes occur in both locations between LS 218° and LS 249°. Modifications are the formation of a new small apron and new deposits within the channel, both associated with the deposition of dark material. Erosion in gully alcoves or channels was not observed. TES data show temperatures between ~180 and ~240 K within the period of gully modifications. Maximum temperatures in the region rise up to ~285 K between LS ~270° and ~310°. Spectral data show a CO2-cover of the study region until LS 227°. CO2-ice free surface are spectrally observed for the first time at LS 249°. H2O was not spectrally detected in the study region and a mixture of CO2 and H2O as presented in [1] cannot be clearly detected. Unfortunately, there are no spectral data available between LS 227° and 249°. Modifications of gullies imply seasonal volatile activity. The activity can be narrowed down to occur between LS 226° and 247° at mean temperatures between ~180 and ~240 K. This is in the range of temperatures where CO2 sublimates back into the atmosphere. Based on the temperature range, the most likely candidate for the observed new

  7. Biology of Trypanosoma (Trypanozoon) evansi in experimental heterologous mammalian hosts.

    PubMed

    Misra, K K; Roy, S; Choudhury, A

    2016-09-01

    Trypanosoma (Trypanozoon) evansi is a causative agent of the dreadful mammalian disease trypanosomiasis or 'Surra' and carried as a latent parasite in domestic cattle but occasionally proves fatal when transmitted to horses and camel. Sporadic outbreak of 'Surra' to different animals (beside their natural hosts) reminds that T. evansi may be zoonotic, as their close relative cause sleeping sickness to human being. This haemoflagellate is mechanically transmitted by horse fly and its effect on different host varies depending on certain factors including the effectiveness of transmission by mechanical vector, the suitability and susceptibility of the host as well as most importantly the ability of the disease establishment of parasite to adapt itself to the host's resistance, etc. The course of the disease caused by T. evansi is similar to that of human sleeping sickness caused by T. (T.) brucei gambiense. The target organs and symptoms show close similarity. T. evansi can successfully be transmitted among unnatural hosts i.e., other classes of vertebrates, like chicken. In transmission experiments, the unnatural hosts may sometimes induce profound changes in the biology of trypanosomes. Hence, in present study the observations are the biology of different morphological changes of T. evansi as well as its ability of disease formation within some heterologous mammal viz., albino rat, guineapig, bandicoot, mongoose, domestic cat and common monkey. Blood smears of infected albino rats, bandicoot, and mongoose revealed only monomorphic form. Interestingly, blood smears of infected cat and monkey, T. evansi shows slender trypomastigote form and short intermediate form whereas organ smears shows other two forms of haemoflagellate viz., sphaeromastigote and amastigote form. The haemoflagellate maintains a common reproductive cycle in all the experimental heterologous hosts whereas disease symptoms differ. T. evansi infected cat and monkey shows nervous symptoms. Infected

  8. A comparative study of prebiotic and present day translational models

    NASA Technical Reports Server (NTRS)

    Rein, R.; Raghunathan, G.; Mcdonald, J.; Shibata, M.; Srinivasan, S.

    1986-01-01

    It is generally recognized that the understanding of the molecular basis of primitive translation is a fundamental step in developing a theory of the origin of life. However, even in modern molecular biology, the mechanism for the decoding of messenger RNA triplet codons into an amino acid sequence of a protein on the ribosome is understood incompletely. Most of the proposed models for prebiotic translation lack, not only experimental support, but also a careful theoretical scrutiny of their compatibility with well understood stereochemical and energetic principles of nucleic acid structure, molecular recognition principles, and the chemistry of peptide bond formation. Present studies are concerned with comparative structural modelling and mechanistic simulation of the decoding apparatus ranging from those proposed for prebiotic conditions to the ones involved in modern biology. Any primitive decoding machinery based on nucleic acids and proteins, and most likely the modern day system, has to satisfy certain geometrical constraints. The charged amino acyl and the peptidyl termini of successive adaptors have to be adjacent in space in order to satisfy the stereochemical requirements for amide bond formation. Simultaneously, the same adaptors have to recognize successive codons on the messenger. This translational complex has to be realized by components that obey nucleic acid conformational principles, stabilities, and specificities. This generalized condition greatly restricts the number of acceptable adaptor structures.

  9. Integrating Competition for Food, Hosts, or Mates via Experimental Evolution.

    PubMed

    Rodrigues, Leonor R; Duncan, Alison B; Clemente, Salomé H; Moya-Laraño, Jordi; Magalhães, Sara

    2016-02-01

    Competitive interactions shape the evolution of organisms. However, often it is not clear whether competition is the driving force behind the patterns observed. The recent use of experimental evolution in competitive environments can help establish such causality. Unfortunately, this literature is scattered, as competition for food, mates, and hosts are subject areas that belong to different research fields. Here, we group these bodies of literature, extract common processes and patterns concerning the role of competition in shaping evolutionary trajectories, and suggest perspectives stemming from an integrative view of competition across these research fields. This review reinstates the power of experimental evolution in addressing the evolutionary consequences of competition, but highlights potential pitfalls in the design of such experiments.

  10. Susceptibility of avian hosts to experimental Gymnophalloides seoi infection.

    PubMed

    Ryang, Y S; Yoo, J C; Lee, S H; Chai, J Y

    2001-04-01

    To determine whether avian species are susceptible to infection with Gymnophalloides seoi (a human-infecting intestinal trematode), we exposed 7 species of birds with metacercariae obtained from oysters. The birds were necropsied at days 2, 4, and 6 postinfection (PI). The highest worm recovery at day 6 PI was obtained from the Kentish plover (Charadrius alexandrinus; mean = 56.0%), followed by the Mongolian plover (C. mongolus; 49.3%), and the grey plover (Pluvialis squatarola; 32.3%). In contrast, no mature worms were recovered from the great knot (Calidris tenuirostris), dunlin (C. alpina), black-tailed gull (Larus crassirostris), and mallard (Anas platyrhynchos). Among the plovers, the worms attained the greatest size at day 6 PI (254.1 x 190.4 microm) in the Kentish plover, with a significantly higher number of eggs in the uterus. The 3 species of plovers are highly susceptible to experimental G. seoi infection, suggesting that they could play a role as definitive hosts for these worms in nature.

  11. Macrophages are required for host survival in experimental urogenital schistosomiasis

    PubMed Central

    Fu, Chi-Ling; Odegaard, Justin I.; Hsieh, Michael H.

    2015-01-01

    Urogenital schistosomiasis, Schistosoma haematobium worm infection, afflicts millions of people with egg-triggered, fibrotic bladder granulomata. Despite the significant global impact of urogenital schistosomiasis, the mechanisms of bladder granulomogenesis and fibrosis are ill defined due to the prior lack of tractable animal models. We combined a mouse model of urogenital schistosomiasis with macrophage-depleting liposomal clodronate (LC) to define how macrophages mediate bladder granulomogenesis and fibrosis. Mice were injected with eggs purified from infected hamsters or vehicle prepared from uninfected hamster tissues (xenoantigen and injection trauma control). Empty liposomes were controls for LC: 1) LC treatment resulted in fewer bladder egg granuloma-infiltrating macrophages, eosinophils, and T and B cells, lower bladder and serum levels of eotaxin, and higher bladder concentrations of IL-1α and chemokines (in a time-dependent fashion), confirming that macrophages orchestrate leukocyte infiltration of the egg-exposed bladder; 2) macrophage-depleted mice exhibited greater weight loss and bladder hemorrhage postegg injection; 3) early LC treatment postegg injection resulted in profound decreases in bladder fibrosis, suggesting differing roles for macrophages in fibrosis over time; and 4) LC treatment also led to egg dose-dependent mortality, indicating that macrophages prevent death from urogenital schistosomiasis. Thus, macrophages are a potential therapeutic target for preventing or treating the bladder sequelae of urogenital schistosomiasis.—Fu, C.-L., Odegaard, J. I., Hsieh, M. H. Macrophages are required for host survival in experimental urogenital schistosomiasis. PMID:25351984

  12. Temporal dynamics of outcrossing and host mortality rates in host-pathogen experimental coevolution.

    PubMed

    Morran, Levi T; Parrish, Raymond C; Gelarden, Ian A; Lively, Curtis M

    2013-07-01

    Cross-fertilization is predicted to facilitate the short-term response and the long-term persistence of host populations engaged in antagonistic coevolutionary interactions. Consistent with this idea, our previous work has shown that coevolving bacterial pathogens (Serratia marcescens) can drive obligately selfing hosts (Caenorhabditis elegans) to extinction, whereas the obligately outcrossing and partially outcrossing populations persisted. We focused the present study on the partially outcrossing (mixed mating) and obligately outcrossing hosts, and analyzed the changes in the host resistance/avoidance (and pathogen infectivity) over time. We found that host mortality rates increased in the mixed mating populations over the first 10 generations of coevolution when outcrossing rates were initially low. However, mortality rates decreased after elevated outcrossing rates evolved during the experiment. In contrast, host mortality rates decreased in the obligately outcrossing populations during the first 10 generations of coevolution, and remained low throughout the experiment. Therefore, predominant selfing reduced the ability of the hosts to respond to coevolving pathogens compared to outcrossing hosts. Thus, we found that host-pathogen coevolution can generate rapid evolutionary change, and that host mating system can influence the outcome of coevolution at a fine temporal scale.

  13. Lymnaea cubensis, an experimental intermediate host for Fascioloides magna.

    PubMed

    Vignoles, Philippe; Novobilský, Adam; Höglund, Johan; Kasný, Martin; Pankrác, Jan; Dreyfuss, Gilles; Pointier, Jean-Pierre; Rondelaud, Daniel

    2014-04-01

    Single-miracidium infections of Lymnaea cubensis (Pfeiffer) from Guadeloupe with the giant liver fluke Fascioloides magna (Bassi, 1875) (Digenea) were carried out during five successive snail generations to determine if this lymnaeid might sustain complete larval development of the parasite. Controls were constituted by a French population of Galba truncatula (Miller) (a single generation) infected according to the same protocol. It was recorded that prevalence and intensity of F. magna infection in L. cubensis progressively increased from F1 to F5 generations. Cercarial shedding of F. magna was noted only within F5 generation of L. cubensis. However, most measured parameters of infection in this species were significantly lower than those noted for G. truncatula and most L. cubensis died after a single shedding wave. Despite this, L. cubensis can be added to the list of potential intermediate hosts of F. magna. PMID:24822325

  14. Minimal effects of latitude on present-day speciation rates in New World birds.

    PubMed

    Rabosky, Daniel L; Title, Pascal O; Huang, Huateng

    2015-06-22

    The tropics contain far greater numbers of species than temperate regions, suggesting that rates of species formation might differ systematically between tropical and non-tropical areas. We tested this hypothesis by reconstructing the history of speciation in New World (NW) land birds using BAMM, a Bayesian framework for modelling complex evolutionary dynamics on phylogenetic trees. We estimated marginal distributions of present-day speciation rates for each of 2571 species of birds. The present-day rate of speciation varies approximately 30-fold across NW birds, but there is no difference in the rate distributions for tropical and temperate taxa. Using macroevolutionary cohort analysis, we demonstrate that clades with high tropical membership do not produce species more rapidly than temperate clades. For nearly any value of present-day speciation rate, there are far more species in the tropics than the temperate zone. Any effects of latitude on speciation rate are marginal in comparison to the dramatic variation in rates among clades.

  15. Present-day secular variations in the zonal harmonics of earth's geopotential

    NASA Technical Reports Server (NTRS)

    Mitrovica, J. X.; Peltier, W. R.

    1993-01-01

    The mathematical formulation required for predicting secular variation in the geopotential is developed for the case of a spherically symmetric, self-gravitating, viscoelastic earth model and an arbitrary surface load which can include a gravitational self-consistent ocean loading component. The theory is specifically applied to predict the present-day secular variation in the zonal harmonics of the geopotenial arising from the surface mass loading associated with the late Pleistocene glacial cycles. A procedure is outlined in which predictions of the present-day geopotential signal due to the late Pleistocene glacial cycles may be used to derive bounds on the net present-day mass flux from the Antarctic and Greenland ice sheets to the local oceans.

  16. Present-day Mars' water cycle: new views and blind perspectives

    NASA Astrophysics Data System (ADS)

    Montmessin, F.; Smith, M. D.; Fedorova, A.; Langevin, Y.; Mellon, M.

    2012-09-01

    Addressing recent cimate changes on Mars necessarily requires a succesful representation of present-day Mars water cycle. Decades of observations and modeling efforts have been conducted that now allow to elaborate a new, yet incomplete, picture, of the seasonal activity of water on Mars. This presentation explores the various observational and theoretical studies that have been conducted to date, and attempts to present a clear and detailed explanation of the major physical mechanisms that command the seasonal and geographical variability of present-day Mars water cycle, as inferred from the combined analysis of measurements and climate model simulations. Remaining issues and enigmae will be presented as well.

  17. The present day formation of apatite in Mexican continental margin sediments

    NASA Astrophysics Data System (ADS)

    Jahnke, Richard A.; Emerson, Steven R.; Roe, Kevin K.; Burnett, William C.

    1983-02-01

    Results of pore water and sediment analyses from the western Mexican continental margin strongly suggest the present day formation of apatite. The interstitial water phosphate and fluoride profiles indicate chemical removal at a depth which corresponds to a large maximum in the phosphorus content of the sediments. Apatite is identified within this maximum via X-ray diffraction but is elsewhere undetectable in the core. Radioisotopic thorium, uranium, and radium data support the conclusion that this deposit is modern. The present day depositional environment is consistent with those reported by other workers for phosphorite formation with the exception that pore water magnesium is not depleted below its seawater value.

  18. Hosting Infection: Experimental Models to Assay Candida Virulence

    PubMed Central

    MacCallum, Donna M.

    2012-01-01

    Although normally commensals in humans, Candida albicans, Candida tropicalis, Candida parapsilosis, Candida glabrata, and Candida krusei are capable of causing opportunistic infections in individuals with altered physiological and/or immunological responses. These fungal species are linked with a variety of infections, including oral, vaginal, gastrointestinal, and systemic infections, with C. albicans the major cause of infection. To assess the ability of different Candida species and strains to cause infection and disease requires the use of experimental infection models. This paper discusses the mucosal and systemic models of infection available to assay Candida virulence and gives examples of some of the knowledge that has been gained to date from these models. PMID:22235206

  19. Future European Summer warming constrained by present-day seasonal cycle

    NASA Astrophysics Data System (ADS)

    Selten, Frank M.; van den Hurk, Bart; Vautard, Robert

    2014-05-01

    European summer temperatures in the CMIP5 multi-model ensemble differ by as much as 5 degrees in the present-day and this spread grows to 9 degrees by the end of this century under the RCP8.5 scenario. This spread is so large since late summer temperatures depend on a number of interacting processes with positive feedbacks during the preceding seasons that are hard to model. These include soil moisture transport and evaporation, atmospheric convection, clouds and circulation. The seasonal cycle is driven by the seasonal change in solar radiation, but these same processes and interactions play a role in the response of the seasonal cycle to an anthropogenically induced change in the radiative forcing. We hypothesize that this is the reason why we find a clear inter-model relationship between the present-day temperature and the projected warming with warm models projecting stronger warming. This hypothesis is confirmed with additional sensitivity simulations with one member of the CMIP5 ensemble (EcEarth); a change in the model formulation of the soil hydrology makes the model warmer in present-day and at the same time increases the projected warming under the RCP8.5 scenario. Based on these results we conclude that the warmest projections are unlikely as these models tend to have a warm bias in the present-day climate.

  20. Historical trauma as public narrative: A conceptual review of how history impacts present-day health

    PubMed Central

    Mohatt, Nathaniel Vincent; Thompson, Azure B.; Thai, Nghi D.; Tebes, Jacob Kraemer

    2014-01-01

    Theories of historical trauma increasingly appear in the literature on individual and community health, especially in relation to racial and ethnic minority populations and groups that experience significant health disparities. As a consequence of this rapid growth, the literature on historical trauma comprises disparate terminology and research approaches. This critical review integrates this literature in order to specify theoretical mechanisms that explain how historical trauma influences the health of individuals and communities. We argue that historical trauma functions as a public narrative for particular groups or communities that connects present-day experiences and circumstances to the trauma so as to influence health. Treating historical trauma as a public narrative shifts the research discourse away from an exclusive search for past causal variables that influence health to identifying how present-day experiences, their corresponding narratives, and their health impacts are connected to public narratives of historical trauma for a particular group or community. We discuss how the connection between historical trauma and present-day experiences, related narratives, and health impacts may function as a source of present-day distress as well as resilience. PMID:24561774

  1. The Determination of the Frequency of Syntactical Patterns in Present-Day Written Australian English.

    ERIC Educational Resources Information Center

    Beebe, Ralph D.

    Confronted with the problem of determining the frequency of syntactical patterns in present-day written Australian English, the author employs a method of analysis which produces an output in the form of a two-dimensional line diagram showing all the syntagms comprising the sentence under analysis. For the remaining problem of sorting the diagrams…

  2. Experimental test of host specificity in a behaviour-modifying trematode.

    PubMed

    Hernandez, R N; Fredensborg, B L

    2015-11-01

    Host behavioural modification by parasites is a common and well-documented phenomenon. However, knowledge on the complexity and specificity of the underlying mechanisms is limited, and host specificity among manipulating parasites has rarely been experimentally verified. We tested the hypothesis that the ability to infect and manipulate host behaviour is restricted to phylogenetically closely related hosts. Our model system consisted of the brain-encysting trematode Euhaplorchis sp. A and six potential fish intermediate hosts from the Order Cyprinodontiformes. Five co-occurring cyprinids were examined for naturally acquired brain infections. Then we selected three species representing three levels of taxonomic relatedness to a known host to experimentally evaluate their susceptibility to infection, and the effect of infection status on behaviours presumably linked to increased trophic transmission. We found natural brain infections of Euhaplorchis sp. A metacercariae in three cyprinids in the shallow sublittoral zone. Of the three experimentally exposed species, Fundulus grandis and Poecilia latipinna acquired infections and displayed an elevated number of conspicuous behaviours in comparison with uninfected controls. Euhaplorchis sp. A was able to infect and manipulate fish belonging to two different families, suggesting that ecological similarity rather than genetic relatedness determines host range in this species. PMID:26394540

  3. Experimental adaptation of Burkholderia cenocepacia to onion medium reduces host range.

    PubMed

    Ellis, Crystal N; Cooper, Vaughn S

    2010-04-01

    It is unclear whether adaptation to a new host typically broadens or compromises host range, yet the answer bears on the fate of emergent pathogens and symbionts. We investigated this dynamic using a soil isolate of Burkholderia cenocepacia, a species that normally inhabits the rhizosphere, is related to the onion pathogen B. cepacia, and can infect the lungs of cystic fibrosis patients. We hypothesized that adaptation of B. cenocepacia to a novel host would compromise fitness and virulence in alternative hosts. We modeled adaptation to a specific host by experimentally evolving 12 populations of B. cenocepacia in liquid medium composed of macerated onion tissue for 1,000 generations. The mean fitness of all populations increased by 78% relative to the ancestor, but significant variation among lines was observed. Populations also varied in several phenotypes related to host association, including motility, biofilm formation, and quorum-sensing function. Together, these results suggest that each population adapted by fixing different sets of adaptive mutations. However, this adaptation was consistently accompanied by a loss of pathogenicity to the nematode Caenorhabditis elegans; by 500 generations most populations became unable to kill nematodes. In conclusion, we observed a narrowing of host range as a consequence of prolonged adaptation to an environment simulating a specific host, and we suggest that emergent pathogens may face similar consequences if they become host-restricted. PMID:20154121

  4. Experimental adaptation of Burkholderia cenocepacia to onion medium reduces host range.

    PubMed

    Ellis, Crystal N; Cooper, Vaughn S

    2010-04-01

    It is unclear whether adaptation to a new host typically broadens or compromises host range, yet the answer bears on the fate of emergent pathogens and symbionts. We investigated this dynamic using a soil isolate of Burkholderia cenocepacia, a species that normally inhabits the rhizosphere, is related to the onion pathogen B. cepacia, and can infect the lungs of cystic fibrosis patients. We hypothesized that adaptation of B. cenocepacia to a novel host would compromise fitness and virulence in alternative hosts. We modeled adaptation to a specific host by experimentally evolving 12 populations of B. cenocepacia in liquid medium composed of macerated onion tissue for 1,000 generations. The mean fitness of all populations increased by 78% relative to the ancestor, but significant variation among lines was observed. Populations also varied in several phenotypes related to host association, including motility, biofilm formation, and quorum-sensing function. Together, these results suggest that each population adapted by fixing different sets of adaptive mutations. However, this adaptation was consistently accompanied by a loss of pathogenicity to the nematode Caenorhabditis elegans; by 500 generations most populations became unable to kill nematodes. In conclusion, we observed a narrowing of host range as a consequence of prolonged adaptation to an environment simulating a specific host, and we suggest that emergent pathogens may face similar consequences if they become host-restricted.

  5. Present-day kinematics of the Rivera plate and implications for tectonics in southwestern Mexico

    NASA Technical Reports Server (NTRS)

    Demets, Charles; Stein, Seth

    1990-01-01

    A model for the present-day motion of the Rivera plate relative to the North America, Cocos, and Pacific plates is derived using new data from the Pacific-Rivera rise and Rivera transform fault, together with new estimates of Pacific-Rivera motions. The results are combined with the closure-consistent NUVEL-1 global plate motion model of DeMets et al. (1990) to examine present-day deformation in southwestern Mexico. The analysis addresses several questions raised in previous studies of the Rivera plate. Namely, do plate motion data from the northern East Pacific rise require a distinct Rivera plate? Do plate kinematic data require the subduction of the Rivera plate along the seismically quiescent Acapulco trench? If so, what does the predicted subduction rate imply about the earthquake recurrence interval in the Jalisco region of southwestern Mexico?

  6. Mantle rheology and satellite signatures from present-day glacial forcings

    NASA Technical Reports Server (NTRS)

    Sabadini, Roberto; Yuen, David A.; Gasperini, Paolo

    1988-01-01

    Changes in the long-wavelength region of the earth's gravity field resulting from both present-day glacial discharges and the possible growth of the Antarctic ice sheet are considered. Significant differences in the responses between the Maxell and Burger body rheologies are found for time spans of less than 100 years. The quantitative model for predicting the secular variations of the gravitational potential, and means for incorporating glacial forcings, are described. Results are given for the excitation of the degree two harmonics. It is suggested that detailed satellite monitoring of present-day ice movements in conjunction with geodetic satellite missions may provide a reasonable alternative for the esimation of deep mantle viscosity.

  7. Experimental evidence for chick discrimination without recognition in a brood parasite host.

    PubMed

    Grim, Tomás

    2007-02-01

    Recognition is considered a critical basis for discriminatory behaviours in animals. Theoretically, recognition and discrimination of parasitic chicks are not predicted to evolve in hosts of brood parasitic birds that evict nest-mates. Yet, an earlier study showed that host reed warblers (Acrocephalus scirpaceus) of an evicting parasite, the common cuckoo (Cuculus canorus), can avoid the costs of prolonged care for unrelated young by deserting the cuckoo chick before it fledges. Desertion was not based on specific recognition of the parasite because hosts accept any chick cross-fostered into their nests. Thus, the mechanism of this adaptive host response remains enigmatic. Here, I show experimentally that the cue triggering this 'discrimination without recognition' behaviour is the duration of parental care. Neither the intensity of brood care nor the presence of a single-chick in the nest could explain desertions. Hosts responded similarly to foreign chicks, whether heterospecific or experimental conspecifics. The proposed mechanism of discrimination strikingly differs from those found in other parasite-host systems because hosts do not need an internal recognition template of the parasite's appearance to effectively discriminate. Thus, host defences against parasitic chicks may be based upon mechanisms qualitatively different from those operating against parasitic eggs. I also demonstrate that this discriminatory mechanism is non-costly in terms of recognition errors. Comparative data strongly suggest that parasites cannot counter-evolve any adaptation to mitigate effects of this host defence. These findings have crucial implications for the process and end-result of host-parasite arms races and our understanding of the cognitive basis of discriminatory mechanisms in general.

  8. Convergence, divergence, and parallelism in marine biodiversity trends: Integrating present-day and fossil data

    PubMed Central

    Huang, Shan; Roy, Kaustuv; Valentine, James W.; Jablonski, David

    2015-01-01

    Paleontological data provide essential insights into the processes shaping the spatial distribution of present-day biodiversity. Here, we combine biogeographic data with the fossil record to investigate the roles of parallelism (similar diversities reached via changes from similar starting points), convergence (similar diversities reached from different starting points), and divergence in shaping the present-day latitudinal diversity gradients of marine bivalves along the two North American coasts. Although both faunas show the expected overall poleward decline in species richness, the trends differ between the coasts, and the discrepancies are not explained simply by present-day temperature differences. Instead, the fossil record indicates that both coasts have declined in overall diversity over the past 3 My, but the western Atlantic fauna suffered more severe Pliocene−Pleistocene extinction than did the eastern Pacific. Tropical western Atlantic diversity remains lower than the eastern Pacific, but warm temperate western Atlantic diversity recovered to exceed that of the temperate eastern Pacific, either through immigration or in situ origination. At the clade level, bivalve families shared by the two coasts followed a variety of paths toward today’s diversities. The drivers of these lineage-level differences remain unclear, but species with broad geographic ranges during the Pliocene were more likely than geographically restricted species to persist in the temperate zone, suggesting that past differences in geographic range sizes among clades may underlie between-coast contrasts. More detailed comparative work on regional extinction intensities and selectivities, and subsequent recoveries (by in situ speciation or immigration), is needed to better understand present-day diversity patterns and model future changes. PMID:25901312

  9. Convergence, divergence, and parallelism in marine biodiversity trends: Integrating present-day and fossil data.

    PubMed

    Huang, Shan; Roy, Kaustuv; Valentine, James W; Jablonski, David

    2015-04-21

    Paleontological data provide essential insights into the processes shaping the spatial distribution of present-day biodiversity. Here, we combine biogeographic data with the fossil record to investigate the roles of parallelism (similar diversities reached via changes from similar starting points), convergence (similar diversities reached from different starting points), and divergence in shaping the present-day latitudinal diversity gradients of marine bivalves along the two North American coasts. Although both faunas show the expected overall poleward decline in species richness, the trends differ between the coasts, and the discrepancies are not explained simply by present-day temperature differences. Instead, the fossil record indicates that both coasts have declined in overall diversity over the past 3 My, but the western Atlantic fauna suffered more severe Pliocene-Pleistocene extinction than did the eastern Pacific. Tropical western Atlantic diversity remains lower than the eastern Pacific, but warm temperate western Atlantic diversity recovered to exceed that of the temperate eastern Pacific, either through immigration or in situ origination. At the clade level, bivalve families shared by the two coasts followed a variety of paths toward today's diversities. The drivers of these lineage-level differences remain unclear, but species with broad geographic ranges during the Pliocene were more likely than geographically restricted species to persist in the temperate zone, suggesting that past differences in geographic range sizes among clades may underlie between-coast contrasts. More detailed comparative work on regional extinction intensities and selectivities, and subsequent recoveries (by in situ speciation or immigration), is needed to better understand present-day diversity patterns and model future changes. PMID:25901312

  10. Convergence, divergence, and parallelism in marine biodiversity trends: Integrating present-day and fossil data.

    PubMed

    Huang, Shan; Roy, Kaustuv; Valentine, James W; Jablonski, David

    2015-04-21

    Paleontological data provide essential insights into the processes shaping the spatial distribution of present-day biodiversity. Here, we combine biogeographic data with the fossil record to investigate the roles of parallelism (similar diversities reached via changes from similar starting points), convergence (similar diversities reached from different starting points), and divergence in shaping the present-day latitudinal diversity gradients of marine bivalves along the two North American coasts. Although both faunas show the expected overall poleward decline in species richness, the trends differ between the coasts, and the discrepancies are not explained simply by present-day temperature differences. Instead, the fossil record indicates that both coasts have declined in overall diversity over the past 3 My, but the western Atlantic fauna suffered more severe Pliocene-Pleistocene extinction than did the eastern Pacific. Tropical western Atlantic diversity remains lower than the eastern Pacific, but warm temperate western Atlantic diversity recovered to exceed that of the temperate eastern Pacific, either through immigration or in situ origination. At the clade level, bivalve families shared by the two coasts followed a variety of paths toward today's diversities. The drivers of these lineage-level differences remain unclear, but species with broad geographic ranges during the Pliocene were more likely than geographically restricted species to persist in the temperate zone, suggesting that past differences in geographic range sizes among clades may underlie between-coast contrasts. More detailed comparative work on regional extinction intensities and selectivities, and subsequent recoveries (by in situ speciation or immigration), is needed to better understand present-day diversity patterns and model future changes.

  11. Convergence, divergence, and parallelism in marine biodiversity trends: Integrating present-day and fossil data

    NASA Astrophysics Data System (ADS)

    Huang, Shan; Roy, Kaustuv; Valentine, James W.; Jablonski, David

    2015-04-01

    Paleontological data provide essential insights into the processes shaping the spatial distribution of present-day biodiversity. Here, we combine biogeographic data with the fossil record to investigate the roles of parallelism (similar diversities reached via changes from similar starting points), convergence (similar diversities reached from different starting points), and divergence in shaping the present-day latitudinal diversity gradients of marine bivalves along the two North American coasts. Although both faunas show the expected overall poleward decline in species richness, the trends differ between the coasts, and the discrepancies are not explained simply by present-day temperature differences. Instead, the fossil record indicates that both coasts have declined in overall diversity over the past 3 My, but the western Atlantic fauna suffered more severe Pliocene-Pleistocene extinction than did the eastern Pacific. Tropical western Atlantic diversity remains lower than the eastern Pacific, but warm temperate western Atlantic diversity recovered to exceed that of the temperate eastern Pacific, either through immigration or in situ origination. At the clade level, bivalve families shared by the two coasts followed a variety of paths toward today's diversities. The drivers of these lineage-level differences remain unclear, but species with broad geographic ranges during the Pliocene were more likely than geographically restricted species to persist in the temperate zone, suggesting that past differences in geographic range sizes among clades may underlie between-coast contrasts. More detailed comparative work on regional extinction intensities and selectivities, and subsequent recoveries (by in situ speciation or immigration), is needed to better understand present-day diversity patterns and model future changes.

  12. Analysis of Present Day and Future OH and Methane Lifetime in the ACCMIP Simulations

    NASA Technical Reports Server (NTRS)

    Voulgarakis, A.; Naik, V.; Lamarque, J. -F.; Shindell, D. T.; Young, P. J.; Prather, M. J.; Wild, O.; Field, R. D.; Bergmann, D.; Cameron-Smith P.; Cionni, I; Collins, W. J.; Dalsoren, S. B.; Doherty, R. M.; Eyring, V.; Faluvegi, G.; Folberth, G. A.; Horowitz, L. W.; Josse, B.; MacKenzie, I. A.; Nagashima, T.; Plummer, D. A.; Righi, M.; Rumbold, S. T.; Strode, S. A.

    2013-01-01

    Results from simulations performed for the Atmospheric Chemistry and Climate Modeling Intercomparison Project (ACCMIP) are analysed to examine how OH and methane lifetime may change from present day to the future, under different climate and emissions scenarios. Present day (2000) mean tropospheric chemical lifetime derived from the ACCMIP multi-model mean is 9.8+/-1.6 yr (9.3+/-0.9 yr when only including selected models), lower than a recent observationally-based estimate, but with a similar range to previous multi-model estimates. Future model projections are based on the four Representative Concentration Pathways (RCPs), and the results also exhibit a large range. Decreases in global methane lifetime of 4.5 +/- 9.1% are simulated for the scenario with lowest radiative forcing by 2100 (RCP 2.6), while increases of 8.5+/-10.4% are simulated for the scenario with highest radiative forcing (RCP 8.5). In this scenario, the key driver of the evolution of OH and methane lifetime is methane itself, since its concentration more than doubles by 2100 and it consumes much of the OH that exists in the troposphere. Stratospheric ozone recovery, which drives tropospheric OH decreases through photolysis modifications, also plays a partial role. In the other scenarios, where methane changes are less drastic, the interplay between various competing drivers leads to smaller and more diverse OH and methane lifetime responses, which are difficult to attribute. For all scenarios, regional OH changes are even more variable, with the most robust feature being the large decreases over the remote oceans in RCP8.5. Through a regression analysis, we suggest that differences in emissions of non-methane volatile organic compounds and in the simulation of photolysis rates may be the main factors causing the differences in simulated present day OH and methane lifetime. Diversity in predicted changes between present day and future OH was found to be associated more strongly with differences in

  13. A data-driven model of present-day glacial isostatic adjustment in North America

    NASA Astrophysics Data System (ADS)

    Simon, Karen; Riva, Riccardo

    2016-04-01

    Geodetic measurements of gravity change and vertical land motion are incorporated into an a priori model of present-day glacial isostatic adjustment (GIA) via least-squares inversion. The result is an updated model of present-day GIA wherein the final predicted signal is informed by both observational data with realistic errors, and prior knowledge of GIA inferred from forward models. This method and other similar techniques have been implemented within a limited but growing number of GIA studies (e.g., Hill et al. 2010). The combination method allows calculation of the uncertainties of predicted GIA fields, and thus offers a significant advantage over predictions from purely forward GIA models. Here, we show the results of using the combination approach to predict present-day rates of GIA in North America through the incorporation of both GPS-measured vertical land motion rates and GRACE-measured gravity observations into the prior model. In order to assess the influence of each dataset on the final GIA prediction, the vertical motion and gravimetry datasets are incorporated into the model first independently (i.e., one dataset only), then simultaneously. Because the a priori GIA model and its associated covariance are developed by averaging predictions from a suite of forward models that varies aspects of the Earth rheology and ice sheet history, the final GIA model is not independent of forward model predictions. However, we determine the sensitivity of the final model result to the prior GIA model information by using different representations of the input model covariance. We show that when both datasets are incorporated into the inversion, the final model adequately predicts available observational constraints, minimizes the uncertainty associated with the forward modelled GIA inputs, and includes a realistic estimation of the formal error associated with the GIA process. Along parts of the North American coastline, improved predictions of the long-term (kyr

  14. Experimental evidence for chick discrimination without recognition in a brood parasite host

    PubMed Central

    Grim, Tomáš

    2006-01-01

    Recognition is considered a critical basis for discriminatory behaviours in animals. Theoretically, recognition and discrimination of parasitic chicks are not predicted to evolve in hosts of brood parasitic birds that evict nest-mates. Yet, an earlier study showed that host reed warblers (Acrocephalus scirpaceus) of an evicting parasite, the common cuckoo (Cuculus canorus), can avoid the costs of prolonged care for unrelated young by deserting the cuckoo chick before it fledges. Desertion was not based on specific recognition of the parasite because hosts accept any chick cross-fostered into their nests. Thus, the mechanism of this adaptive host response remains enigmatic. Here, I show experimentally that the cue triggering this ‘discrimination without recognition’ behaviour is the duration of parental care. Neither the intensity of brood care nor the presence of a single-chick in the nest could explain desertions. Hosts responded similarly to foreign chicks, whether heterospecific or experimental conspecifics. The proposed mechanism of discrimination strikingly differs from those found in other parasite–host systems because hosts do not need an internal recognition template of the parasite's appearance to effectively discriminate. Thus, host defences against parasitic chicks may be based upon mechanisms qualitatively different from those operating against parasitic eggs. I also demonstrate that this discriminatory mechanism is non-costly in terms of recognition errors. Comparative data strongly suggest that parasites cannot counter-evolve any adaptation to mitigate effects of this host defence. These findings have crucial implications for the process and end-result of host–parasite arms races and our understanding of the cognitive basis of discriminatory mechanisms in general. PMID:17164201

  15. Utilizing Present-Day Stable Water Isotopes to Improve Paleoclimate Records from the Southeast (USA)

    NASA Astrophysics Data System (ADS)

    McKay, K. K.; Lambert, W. J.

    2015-12-01

    Present-day water isotope data are used to help interpret climate (paleo-rainfall) proxies archived in the geologic record, which can then aid in the creation of General Circulation Models (GCM). The Southeast (USA) is under-represented with respect to present-day measurement of water isotopes and high-resolution paleoclimate records, thus GCMs must extrapolate data for the region. We will evaluate water isotope data (δ18O, δD) collected and analyzed at The University of Alabama (33°13'N, 87°33'W) since June 2005. The monitoring station, central to the Southeast, was established to provide long-term water isotope data needed for reconstructing paleo-rainfall records of the region. Proxy data (e.g., δ18Ocalcite) archived in speleothems have been demonstrated to provide trustworthy information about past climate conditions; however, present-day monitoring of both local rainfall and cave dripwater are crucial. The decade-long (June 2005 - May 2015) rainfall record allows for the establishment of the relationship between water isotopes (δ18O, δD) and monthly air temperature, rainfall amount, as well as the general differences between summer and winter rainfall. Dripwater from Cathedral Caverns (34°34'N, 86°13'W), located in northeastern Alabama, has been sampled at a monthly resolution since January 2015 to determine if the water chemistry in the cave represents an annual mean for the rainfall or if it is seasonally biased. The ultimate goal of this study is to better understand how atmospheric air currents (specifically the strength/position of the Polar Jet Stream, PJS), and hence rainfall in the Southeast, varied during past periods of relative warming (e.g., Dansgaard-Oeschger events) and cooling (e.g., Heinrich events) of the Northern Hemisphere atmosphere. Future GCMs will be improved if a reliable high-resolution paleo-rainfall record can be produced for the Southeast.

  16. Comparison of lactase persistence polymorphism in ancient and present-day Hungarian populations.

    PubMed

    Nagy, Dóra; Tömöry, Gyöngyvér; Csányi, Bernadett; Bogácsi-Szabó, Erika; Czibula, Ágnes; Priskin, Katalin; Bede, Olga; Bartosiewicz, László; Downes, C Stephen; Raskó, István

    2011-06-01

    The prevalence of adult-type hypolactasia varies ethnically and geographically among populations. A C/T-13910 single nucleotide polymorphism (SNP) upstream of the lactase gene is known to be associated with lactase non-persistence in Europeans. The aim of this study was to determine the prevalence of lactase persistent and non-persistent genotypes in current Hungarian-speaking populations and in ancient bone samples of classical conquerors and commoners from the 10th-11th centuries from the Carpathian basin; 181 present-day Hungarian, 65 present-day Sekler, and 23 ancient samples were successfully genotyped for the C/T-13910 SNP by the dCAPS PCR-RFLP method. Additional mitochondrial DNA testing was also carried out. In ancient Hungarians, the T-13910 allele was present only in 11% of the population, and exclusively in commoners of European mitochondrial haplogroups who may have been of pre-Hungarian indigenous ancestry. This is despite animal domestication and dairy products having been introduced into the Carpathian basin early in the Neolithic Age. This anomaly may be explained by the Hungarian use of fermented milk products, their greater consumption of ruminant meat than milk, cultural differences, or by their having other lactase-regulating genetic polymorphisms than C/T-13910. The low prevalence of lactase persistence provides additional information on the Asian origin of Hungarians. Present-day Hungarians have been assimilated with the surrounding European populations, since they do not differ significantly from the neighboring populations in their possession of mtDNA and C/T-13910 variants.

  17. Comparison of lactase persistence polymorphism in ancient and present-day Hungarian populations.

    PubMed

    Nagy, Dóra; Tömöry, Gyöngyvér; Csányi, Bernadett; Bogácsi-Szabó, Erika; Czibula, Ágnes; Priskin, Katalin; Bede, Olga; Bartosiewicz, László; Downes, C Stephen; Raskó, István

    2011-06-01

    The prevalence of adult-type hypolactasia varies ethnically and geographically among populations. A C/T-13910 single nucleotide polymorphism (SNP) upstream of the lactase gene is known to be associated with lactase non-persistence in Europeans. The aim of this study was to determine the prevalence of lactase persistent and non-persistent genotypes in current Hungarian-speaking populations and in ancient bone samples of classical conquerors and commoners from the 10th-11th centuries from the Carpathian basin; 181 present-day Hungarian, 65 present-day Sekler, and 23 ancient samples were successfully genotyped for the C/T-13910 SNP by the dCAPS PCR-RFLP method. Additional mitochondrial DNA testing was also carried out. In ancient Hungarians, the T-13910 allele was present only in 11% of the population, and exclusively in commoners of European mitochondrial haplogroups who may have been of pre-Hungarian indigenous ancestry. This is despite animal domestication and dairy products having been introduced into the Carpathian basin early in the Neolithic Age. This anomaly may be explained by the Hungarian use of fermented milk products, their greater consumption of ruminant meat than milk, cultural differences, or by their having other lactase-regulating genetic polymorphisms than C/T-13910. The low prevalence of lactase persistence provides additional information on the Asian origin of Hungarians. Present-day Hungarians have been assimilated with the surrounding European populations, since they do not differ significantly from the neighboring populations in their possession of mtDNA and C/T-13910 variants. PMID:21365615

  18. Experimentally induced host-shift changes life-history strategy in a seed beetle.

    PubMed

    Savković, Uroš; ĐorĐević, Mirko; Šešlija Jovanović, Darka; Lazarević, Jelica; Tucić, Nikola; Stojković, Biljana

    2016-04-01

    Expansion of the host range in phytophagous insects depends on their ability to form an association with a novel plant through changes in host-related traits. Phenotypic plasticity has important effects on initial survival of individuals faced with a new plant, as well as on the courses of evolutionary change during long-term adaptation to novel conditions. Using experimental populations of the seed beetle that evolved on ancestral (common bean) or novel (chickpea) host and applying reciprocal transplant at both larval and adult stage on the alternative host plant, we studied the relationship between the initial (plastic) phases of host-shift and the subsequent stages of evolutionary divergence in life-history strategies between populations exposed to the host-shift process. After 48 generations, populations became well adapted to chickpea by evolving the life-history strategy with prolonged larval development, increased body mass, earlier reproduction, shorter lifespan and decreased plasticity of all traits compared with ancestral conditions. In chickpea-adapted beetles, negative fitness consequences of low plasticity of pre-adult development (revealed as severe decrease in egg-to-adult viability on beans) exhibited mismatch with positive effects of low plasticity (i.e. low host sensitivity) in oviposition and fecundity. In contrast, beetles adapted to the ancestral host showed high plasticity of developmental process, which enabled high larval survival on chickpea, whereas elevated plasticity in adult behaviour (i.e. high host sensitivity) resulted in delayed reproduction and decreased fecundity on chickpea. The analysis of population growth parameters revealed significant fluctuation during successive phases of the host-shift process in A. obtectus. PMID:26790127

  19. Functional Analyses of Transcription Factor Binding Sites that Differ between Present-Day and Archaic Humans

    PubMed Central

    Weyer, Sven; Pääbo, Svante

    2016-01-01

    We analyze 25 previously identified transcription factor binding sites that carry DNA sequence changes that are present in all or nearly all present-day humans, yet occur in the ancestral state in Neandertals and Denisovans, the closest evolutionary relatives of humans. When the ancestral and derived forms of the transcription factor binding sites are tested using reporter constructs in 3 neuronal cell lines, the activity of 12 of the derived versions of transcription factor binding sites differ from the respective ancestral variants. This suggests that the majority of this class of evolutionary differences between modern humans and Neandertals may affect gene expression in at least some tissue or cell type. PMID:26454764

  20. Present-day Exposures of Water Ice in the Northern Mid-latitudes of Mars

    NASA Technical Reports Server (NTRS)

    Allen, Carlton C.; Kanner, Lisa C.

    2007-01-01

    Water ice is exposed in the martian north polar cap, but is rarely exposed beyond the cap boundary. Orbital gamma ray spectrometry data strongly imply the presence of water ice within meters of the surface at latitudes north of approximately 60deg. We have examined mid-latitude areas of the northern plains displaying residual ice-rich layers, and report evidence of present-day surface exposures of water ice. These exposures, if confirmed, could con-strain the latitudinal and temporal stability of surface ice on Mars.

  1. Experimental Models to Study the Role of Microbes in Host-Parasite Interactions

    PubMed Central

    Hahn, Megan A.; Dheilly, Nolwenn M.

    2016-01-01

    Until recently, parasitic infections have been primarily studied as interactions between the parasite and the host, leaving out crucial players: microbes. The recent realization that microbes play key roles in the biology of all living organisms is not only challenging our understanding of host-parasite evolution, but it also provides new clues to develop new therapies and remediation strategies. In this paper we provide a review of promising and advanced experimental organismal systems to examine the dynamic of host-parasite-microbe interactions. We address the benefits of developing new experimental models appropriate to this new research area and identify systems that offer the best promises considering the nature of the interactions among hosts, parasites, and microbes. Based on these systems, we identify key criteria for selecting experimental models to elucidate the fundamental principles of these complex webs of interactions. It appears that no model is ideal and that complementary studies should be performed on different systems in order to understand the driving roles of microbes in host and parasite evolution. PMID:27602023

  2. Experimental Models to Study the Role of Microbes in Host-Parasite Interactions.

    PubMed

    Hahn, Megan A; Dheilly, Nolwenn M

    2016-01-01

    Until recently, parasitic infections have been primarily studied as interactions between the parasite and the host, leaving out crucial players: microbes. The recent realization that microbes play key roles in the biology of all living organisms is not only challenging our understanding of host-parasite evolution, but it also provides new clues to develop new therapies and remediation strategies. In this paper we provide a review of promising and advanced experimental organismal systems to examine the dynamic of host-parasite-microbe interactions. We address the benefits of developing new experimental models appropriate to this new research area and identify systems that offer the best promises considering the nature of the interactions among hosts, parasites, and microbes. Based on these systems, we identify key criteria for selecting experimental models to elucidate the fundamental principles of these complex webs of interactions. It appears that no model is ideal and that complementary studies should be performed on different systems in order to understand the driving roles of microbes in host and parasite evolution. PMID:27602023

  3. Do malaria parasites manipulate the escape behaviour of their avian hosts? An experimental study.

    PubMed

    Garcia-Longoria, Luz; Møller, Anders P; Balbontín, Javier; de Lope, Florentino; Marzal, Alfonso

    2015-12-01

    Escape behaviour is the behaviour that birds and other animals display when already caught by a predator. An individual exhibiting higher intensity of such anti-predator behaviour could have greater probabilities of escape from predators. Parasites are known to affect different aspects of host behaviour to increase their own fitness. Vector-transmitted parasites such as malaria parasites should gain by manipulating their hosts to enhance the probability of transmission. Several studies have shown that malaria parasites can manipulate their vectors leading to increased transmission success. However, little is known about whether malaria parasites can manipulate escape behaviour of their avian hosts thereby increasing the spread of the parasite. Here we used an experimental approach to explore if Plasmodium relictum can manipulate the escape behaviour of one of its most common avian hosts, the house sparrow Passer domesticus. We experimentally tested whether malaria parasites manipulate the escape behaviour of their avian host. We showed a decrease in the intensity of biting and tonic immobility after removal of infection with anti-malaria medication compared to pre-experimental behaviour. These outcomes suggest that infected sparrows performed more intense escape behaviour, which would increase the likelihood of individuals escaping from predators, but also benefit the parasite by increasing its transmission opportunities.

  4. Experimental Models to Study the Role of Microbes in Host-Parasite Interactions

    PubMed Central

    Hahn, Megan A.; Dheilly, Nolwenn M.

    2016-01-01

    Until recently, parasitic infections have been primarily studied as interactions between the parasite and the host, leaving out crucial players: microbes. The recent realization that microbes play key roles in the biology of all living organisms is not only challenging our understanding of host-parasite evolution, but it also provides new clues to develop new therapies and remediation strategies. In this paper we provide a review of promising and advanced experimental organismal systems to examine the dynamic of host-parasite-microbe interactions. We address the benefits of developing new experimental models appropriate to this new research area and identify systems that offer the best promises considering the nature of the interactions among hosts, parasites, and microbes. Based on these systems, we identify key criteria for selecting experimental models to elucidate the fundamental principles of these complex webs of interactions. It appears that no model is ideal and that complementary studies should be performed on different systems in order to understand the driving roles of microbes in host and parasite evolution.

  5. Using present-day observations to detect when anthropogenic change forces surface ocean carbonate chemistry outside preindustrial bounds

    NASA Astrophysics Data System (ADS)

    Sutton, Adrienne J.; Sabine, Christopher L.; Feely, Richard A.; Cai, Wei-Jun; Cronin, Meghan F.; McPhaden, Michael J.; Morell, Julio M.; Newton, Jan A.; Noh, Jae-Hoon; Ólafsdóttir, Sólveig R.; Salisbury, Joseph E.; Send, Uwe; Vandemark, Douglas C.; Weller, Robert A.

    2016-09-01

    One of the major challenges to assessing the impact of ocean acidification on marine life is detecting and interpreting long-term change in the context of natural variability. This study addresses this need through a global synthesis of monthly pH and aragonite saturation state (Ωarag) climatologies for 12 open ocean, coastal, and coral reef locations using 3-hourly moored observations of surface seawater partial pressure of CO2 and pH collected together since as early as 2010. Mooring observations suggest open ocean subtropical and subarctic sites experience present-day surface pH and Ωarag conditions outside the bounds of preindustrial variability throughout most, if not all, of the year. In general, coastal mooring sites experience more natural variability and thus, more overlap with preindustrial conditions; however, present-day Ωarag conditions surpass biologically relevant thresholds associated with ocean acidification impacts on Mytilus californianus (Ωarag < 1.8) and Crassostrea gigas (Ωarag < 2.0) larvae in the California Current Ecosystem (CCE) and Mya arenaria larvae in the Gulf of Maine (Ωarag < 1.6). At the most variable mooring locations in coastal systems of the CCE, subseasonal conditions approached Ωarag = 1. Global and regional models and data syntheses of ship-based observations tended to underestimate seasonal variability compared to mooring observations. Efforts such as this to characterize all patterns of pH and Ωarag variability and change at key locations are fundamental to assessing present-day biological impacts of ocean acidification, further improving experimental design to interrogate organism response under real-world conditions, and improving predictive models and vulnerability assessments seeking to quantify the broader impacts of ocean acidification.

  6. Present-day nearshore pH differentially depresses fertilization in congeneric sea urchins.

    PubMed

    Frieder, Christina A

    2014-02-01

    Ocean acidification impacts fertilization in some species of sea urchin, but whether sensitivity is great enough to be influenced by present-day pH variability has not been documented. In this study, fertilization in two congeneric sea urchins, Strongylocentrotus purpuratus and S. franciscanus, was found to be sensitive to reduced pH, <7.50, but only within a range of sperm-egg ratios that was species-specific. By further testing fertilization across a broad range of pH, pH-fertilization curves were generated and revealed that S. purpuratus was largely robust to pH, while fertilization in S. franciscanus was sensitive to even modest reductions in pH. Combining the pH-fertilization response curves with pH data collected from these species' habitat demonstrated that relative fertilization success remained high for S. purpuratus but could be as low as 79% for S. franciscanus during periods of naturally low pH. In order for S. franciscanus to maintain high fertilization success in the present and future, adequate adult densities, and thus sufficient sperm-egg ratios, will be required to negate the effects of low pH. In contrast, fertilization of S. purpuratus was robust to a broad range of pH, encompassing both present-day and future ocean acidification scenarios, even though the two congeners have similar habitats.

  7. Mitogenomes from The 1000 Genome Project Reveal New Near Eastern Features in Present-Day Tuscans

    PubMed Central

    Pardo-Seco, Jacobo; Amigo, Jorge; Martinón-Torres, Federico

    2015-01-01

    Background Genetic analyses have recently been carried out on present-day Tuscans (Central Italy) in order to investigate their presumable recent Near East ancestry in connection with the long-standing debate on the origins of the Etruscan civilization. We retrieved mitogenomes and genome-wide SNP data from 110 Tuscans analyzed within the context of The 1000 Genome Project. For phylogeographic and evolutionary analysis we made use of a large worldwide database of entire mitogenomes (>26,000) and partial control region sequences (>180,000). Results Different analyses reveal the presence of typical Near East haplotypes in Tuscans representing isolated members of various mtDNA phylogenetic branches. As a whole, the Near East component in Tuscan mitogenomes can be estimated at about 8%; a proportion that is comparable to previous estimates but significantly lower than admixture estimates obtained from autosomal SNP data (21%). Phylogeographic and evolutionary inter-population comparisons indicate that the main signal of Near Eastern Tuscan mitogenomes comes from Iran. Conclusions Mitogenomes of recent Near East origin in present-day Tuscans do not show local or regional variation. This points to a demographic scenario that is compatible with a recent arrival of Near Easterners to this region in Italy with no founder events or bottlenecks. PMID:25786119

  8. Ancient DNA reveals matrilineal continuity in present-day Poland over the last two millennia.

    PubMed

    Juras, Anna; Dabert, Miroslawa; Kushniarevich, Alena; Malmström, Helena; Raghavan, Maanasa; Kosicki, Jakub Z; Metspalu, Ene; Willerslev, Eske; Piontek, Janusz

    2014-01-01

    While numerous ancient human DNA datasets from across Europe have been published till date, modern-day Poland in particular, remains uninvestigated. Besides application in the reconstruction of continent-wide human history, data from this region would also contribute towards our understanding of the history of the Slavs, whose origin is hypothesized to be in East or Central Europe. Here, we present the first population-scale ancient human DNA study from the region of modern-day Poland by establishing mitochondrial DNA profiles for 23 samples dated to 200 BC - 500 AD (Roman Iron Age) and for 20 samples dated to 1000-1400 AD (Medieval Age). Our results show that mitochondrial DNA sequences from both periods belong to haplogroups that are characteristic of contemporary West Eurasia. Haplotype sharing analysis indicates that majority of the ancient haplotypes are widespread in some modern Europeans, including Poles. Notably, the Roman Iron Age samples share more rare haplotypes with Central and Northeast Europeans, whereas the Medieval Age samples share more rare haplotypes with East-Central and South-East Europeans, primarily Slavic populations. Our data demonstrates genetic continuity of certain matrilineages (H5a1 and N1a1a2) in the area of present-day Poland from at least the Roman Iron Age until present. As such, the maternal gene pool of present-day Poles, Czechs and Slovaks, categorized as Western Slavs, is likely to have descended from inhabitants of East-Central Europe during the Roman Iron Age. PMID:25337992

  9. Chemical interactions between the present-day Martian atmosphere and surface minerals: Implications for sample return

    NASA Technical Reports Server (NTRS)

    Prinn, Ronald; Fegley, Bruce

    1988-01-01

    Thermochemical and photochemical reactions between surface minerals and present-day atmospheric constituents are predicted to produce microscopic effects on the surface of mineral grains. Relevant reactions hypothesized in the literature include conversions of silicates and volcanic glasses to clay minerals, conversion of ferrous to ferric compounds, and formation of carbonates, nitrates, and sulfates. These types of surface-atmosphere weathering of minerals, biological potential of the surface environment, and atmospheric stability in both present and past Martian epochs. It is emphasized that the product of these reactions will be observable and interpretable on the microscopic surface layers of Martian surface rocks using modern techniques with obvious implications for sample return from Mars. Macroscopic products of chemical weathering reactions in past Martian epochs are also expected in Martian surface materials. These products are expected not only as a result of reactions similar to those proceeding today but also due to aqueous reactions in past epochs in which liquid water was putatively present. It may prove very difficult or impossible, however, to determine definitively from the relic macroscopic product alone either the exact weathering process which led to its formation of the identity of its weathering parent mineral. The enormous advantages of studying the Martian chemical weathering by investigating the microscopic products of present-day chemical reactions on sample surfaces are very apparent.

  10. Chemical interactions between the present-day Martian atmosphere and surface minerals

    NASA Technical Reports Server (NTRS)

    Prinn, Ronald; Fegley, Bruce

    1987-01-01

    Thermochemical and photochemical reactions between surface minerals and present-day atmospheric constituents are predicted to produce microscopic effects on the surfaces of mineral grains. Relevant reactions hypothesized in the literature include conversions of silicates and volcanic glasses to clay minerals, conversion of ferrous to ferric compounds, and formation of carbonates, nitrates, and sulfates. These types of surface-atmosphere interactions are important for addressing issues such as chemical weathering of minerals, biological potential of the surface environment, and atmospheric stability in both present and past Martian epochs. It is emphasized that the product of these reactions will be observable and interpretable on the microscopic surface layers of Martian surface rocks using modern techniques with obvious implications for sample return from Mars. Macroscopic products of chemical weathering reactions in past Martian epochs are also expected in Martian surface material. These products are expected not only as a result of reactions similar to those proceeding today but also due to aqueous reactions in past epochs in which liquid water was putatively present. It may prove very difficult or impossible however to determine definitively from the relic macroscopic product alone either the exact weathering process which led to its formation or the identity of its weathered parent mineral. The enormous advantages of studying Martian chemical weathering by investigating the microscopic products of present-day chemical reactions on sample surfaces are very apparent.

  11. Present-day and future global bottom-up ship emission inventories including polar routes.

    PubMed

    Paxian, Andreas; Eyring, Veronika; Beer, Winfried; Sausen, Robert; Wright, Claire

    2010-02-15

    We present a global bottom-up ship emission algorithm that calculates fuel consumption, emissions, and vessel traffic densities for present-day (2006) and two future scenarios (2050) considering the opening of Arctic polar routes due to projected sea ice decline. Ship movements and actual ship engine power per individual ship from Lloyd's Marine Intelligence Unit (LMIU) ship statistics for six months in 2006 and further mean engine data from literature serve as input. The developed SeaKLIM algorithm automatically finds the most probable shipping route for each combination of start and destination port of a certain ship movement by calculating the shortest path on a predefined model grid while considering land masses, sea ice, shipping canal sizes, and climatological mean wave heights. The resulting present-day ship activity agrees well with observations. The global fuel consumption of 221 Mt in 2006 lies in the range of previously published inventories when undercounting of ship numbers in the LMIU movement database (40,055 vessels) is considered. Extrapolated to 2007 and ship numbers per ship type of the recent International Maritime Organization (IMO) estimate (100,214 vessels), a fuel consumption of 349 Mt is calculated which is in good agreement with the IMO total of 333 Mt. The future scenarios show Arctic polar routes with regional fuel consumption on the Northeast and Northwest Passage increasing by factors of up to 9 and 13 until 2050, respectively. PMID:20088494

  12. Earthquake slip vectors and estimates of present-day plate motions

    NASA Technical Reports Server (NTRS)

    Demets, Charles

    1993-01-01

    Two alternative models for present-day global plate motions are derived from subsets of the NUVEL-1 data in order to investigate the degree to which earthquake slip vectors affect the NUVEL-1 model and to provide estimates of present-day plate velocities that are independent of earthquake slip vectors. The data set used to derive the first model excludes subduction zone slip vectors. The primary purpose of this model is to demonstrate that the 240 subduction zone slip vectors in the NUVEL-1 data set do not greatly affect the plate velocities predicted by NUVEL-1. A data set that excludes all of the 724 earthquake slip vectors used to derive NUVEL-1 is used to derive the second model. This model is suitable as a reference model for kinematic studies that require plate velocity estimates unaffected by earthquake slip vectors. The slip-dependent slip vector bias along transform faults is investigated using the second model, and evidence is sought for biases in slip directions along spreading centers.

  13. A non-tectonic origin for the present day stress field in the sedimentary Paris Basin

    NASA Astrophysics Data System (ADS)

    Cornet, Francois; Magnenet, Vincent

    2016-04-01

    The large scale stress patterns observed in intraplate area is generally considered to result from far-field boundary forces that drive plate tectonics. However, no present day deformation has been detected in the Paris Basin, yet significant deviatoric stresses are measured in limestone formations observed above soft argillite layers encountered in this region at depths close to 500m. Further, the pore pressure measured in the argillite is larger than that measured in the surrounding permeable zones. These observations suggest a presently active source of stress in this sedimentary system. We propose that this stress is not related to tectonics but to pressure solution effects activated by pore pressure transients. These transients develop in the natural fracture system that affects the limestone formations. They are linked to climatic variations and involve periods that range from thousands to hundreds of thousands years. This mechanism generates time-dependent shear stresses in soft formations and explains overpressures observed in the very low permeability argillite. This mechanism may be modeled by different visco-elastic behaviors for the various formations. It outlines the influence of time dependent material properties on the present day stress field. These results imply that the viscoelastic properties of sedimentary formations raise a strong difficulty for extrapolating measured surface deformations to basement rocks in domains of very slow tectonics.

  14. Present-day and future global bottom-up ship emission inventories including polar routes.

    PubMed

    Paxian, Andreas; Eyring, Veronika; Beer, Winfried; Sausen, Robert; Wright, Claire

    2010-02-15

    We present a global bottom-up ship emission algorithm that calculates fuel consumption, emissions, and vessel traffic densities for present-day (2006) and two future scenarios (2050) considering the opening of Arctic polar routes due to projected sea ice decline. Ship movements and actual ship engine power per individual ship from Lloyd's Marine Intelligence Unit (LMIU) ship statistics for six months in 2006 and further mean engine data from literature serve as input. The developed SeaKLIM algorithm automatically finds the most probable shipping route for each combination of start and destination port of a certain ship movement by calculating the shortest path on a predefined model grid while considering land masses, sea ice, shipping canal sizes, and climatological mean wave heights. The resulting present-day ship activity agrees well with observations. The global fuel consumption of 221 Mt in 2006 lies in the range of previously published inventories when undercounting of ship numbers in the LMIU movement database (40,055 vessels) is considered. Extrapolated to 2007 and ship numbers per ship type of the recent International Maritime Organization (IMO) estimate (100,214 vessels), a fuel consumption of 349 Mt is calculated which is in good agreement with the IMO total of 333 Mt. The future scenarios show Arctic polar routes with regional fuel consumption on the Northeast and Northwest Passage increasing by factors of up to 9 and 13 until 2050, respectively.

  15. Present-day crustal deformation along the El Salvador Fault Zone from ZFESNet GPS network

    NASA Astrophysics Data System (ADS)

    Staller, Alejandra; Martínez-Díaz, José Jesús; Benito, Belén; Alonso-Henar, Jorge; Hernández, Douglas; Hernández-Rey, Román; Díaz, Manuel

    2016-02-01

    This paper presents the results and conclusions obtained from new GPS data compiled along the El Salvador Fault Zone (ESFZ). We calculated a GPS-derived horizontal velocity field representing the present-day crustal deformation rates in the ESFZ based on the analysis of 30 GPS campaign stations of the ZFESNet network, measured over a 4.5 year period from 2007 to 2012. The velocity field and subsequent strain rate analysis clearly indicate dextral strike-slip tectonics with extensional component throughout the ESFZ. Our results suggest that the boundary between the Salvadoran forearc and Caribbean blocks is a deformation zone which varies along the fault zone. We estimate that the movement between the two blocks is at least ~ 12 mm yr- 1. From west to east, this movement is variably distributed between faults or segments of the ESFZ. We propose a kinematic model with three main blocks; the Western, Central and Eastern blocks delimited by major faults. For the first time, we were able to provide a quantitative measure of the present-day horizontal geodetic slip rate of the main segments of ESFZ, ranging from ~ 2 mm yr- 1 in the east segment to ~ 8 mm yr- 1, in the west and central segments. This study contributes new kinematic and slip rate data that should be used to update and improve the seismic hazard assessments in northern Central America.

  16. The present-day geodynamics of the India-Asia collision system

    NASA Astrophysics Data System (ADS)

    Baumann, Tobias; Kaus, Boris

    2016-04-01

    We present a full 3D geodynamic model of the present-day India-Asia collision system, that includes the lithosphere and upper mantle. The model is separated into multiple tectonic blocks, for which we estimate the first order rheological properties and their impact on the dynamics of the collision system. This is done by performing systematic simulations with different rheologies to minimize the misfit to observational constraints such as the GPS-velocity field. The simulations are performed with the parallel staggered grid FD code LaMEM using a numerical resolution of at least 512x512x256 cells to resolve dynamically important shear zones reasonably well. A fundamental part of this study is the reconstruction of the 3D present-day geometry of Tibet and the adjacent regions. Our interpretations of crust and mantle lithosphere geometry are jointly based on a globally available shear wave tomography and a global crustal model. We regionally refined and modified our interpretations based on seismicity distributions and focal mechanisms and incorporated regional receiver function studies to improve the accuracy of the Moho in particular. Results suggest that we can identify at least one 'best-fit' solution in terms of rheological model properties that reproduces the observed velocity field reasonably well, including the strong rotation of the GPS velocity around the eastern syntaxis of the Himalaya. We also present model co-variances.

  17. Past- and present-day Madden-Julian Oscillation in CNRM-CM5

    NASA Astrophysics Data System (ADS)

    Song, Eun-Ji; Seo, Kyong-Hwan

    2016-04-01

    Madden-Julian Oscillation (MJO) in the past (nineteenth century) and present day (twentieth century) is examined using preindustrial and historical experiments of Centre National de Recherches Météorologiques-Coupled Models, version 5 (CNRM-CM5) in Coupled Model Intercomparison Project Phase 5 (CMIP5). The present-day MJO is stronger than the past MJO by 33% and it is ~10% more frequent. In particular, the MJO phases 4-7 signifying deep convection situated over the Maritime continent and western Pacific (WP) are considerably enhanced. These changes are due mainly to greenhouse gas forcing with little impact from nature forcing. Dynamical mechanisms for this change are investigated. A peculiar strengthening of MJO over WP comes from increased basic-state sea surface temperature (SST) over the Central Pacific (CP) and EP. The increase in precipitation over WP results from both the response to enhanced SST over CP and the inverted Walker circulation induced by the EP and CP SST increase. The latter causes a pair of anticyclonic Rossby waves straddling the equator, leading to moisture convergence over WP.

  18. Biomarker patterns in present-day vegetation: consistency and variation - A study on plaggen soils

    NASA Astrophysics Data System (ADS)

    Kirkels, Frédérique; Jansen, Boris; Kalbitz, Karsten

    2013-04-01

    Biomarker patterns in present-day vegetation are commonly used as proxies to reconstruct paleo-vegetation composition, land use history and to elucidate carbon cycling. Plaggen soils are formed by diverse vegetational inputs during century-long plaggen (i.e. sod) application associated with plaggen-agriculture on poor soils in north-western Europe. This resulted in remarkably stable organic matter. Plant source identification by biomarkers could provide insight in yet unknown stabilization mechanisms and the fate of organic matter upon ongoing land use change. The current rationale behind biomarker-based source identification is that patterns observed in present-day vegetation are generally representative with little random variation. However, our knowledge on variability and consistency of biomarker patterns is yet scarce. Therefore, to assess the applicability of biomarkers for source identification in plaggen soils, we analyzed published n-alkane and n-alcohol patterns of species and their various parts which contribute(d) input to plaggen soils. We considered shrubs, trees and grass species and evaluated rescaled patterns (i.e. relative abundances in chain-length range C17-36), odd-over-even predominance (OEP) and predominant n-alkanes. In addition, we explicitly looked into potential sources of systematic variation, e.g. spatial variation (climate, site conditions), temporal variation (seasonality, ontogeny) and laboratory methodology (extraction technique: washing/shaking, Soxhlet/ASE, saponification). We found meaningful clustering of n-alkanes C27, C29, C31 and C33, allowing for clear distinction of input by shrubs, trees and grasses to plaggen soils. Combination of these homologues with complete n-alkane patterns (C17-36) and OEP enabled further differentiation, while n-alcohols patterns were less distinct. Current limitation is the lack of extended and diverse quantitative records on biomarker patterns, especially for n-alcohols, non-leaf and belowground

  19. Three-dimensional instantaneous dynamics modeling of present-day Aegean subduction

    NASA Astrophysics Data System (ADS)

    Glerum, Anne; Thieulot, Cedric; Pranger, Casper; van Hinsbergen, Douwe; Fraters, Menno; Spakman, Wim

    2015-04-01

    The Aegean region (Eastern Mediterranean) is exemplary of the interaction between crustal tectonics, plate motion, subduction and mantle flow: African subduction underneath the region has been continuous for at least the last 100 My, leading to about 2100-2500 km of subducted lithosphere residing in the mantle (van Hinsbergen et al., 2005). During this subduction, decoupled upper continental and oceanic crust accreted into a wedge of stacked nappes. In turn, these nappes have been significantly extended, predominantly during the last 25 My, due to the retreat of the African slab relative to Eurasia (van Hinsbergen and Schmid, 2012). As a first step to better understanding the coupling of the tectonic evolution of the crust and the underlying mantle dynamics, we are developing 3-D numerical models of the instantaneous dynamics of the present-day Aegean subduction system using the finite element code ASPECT (Kronbichler et al., 2012). The instantaneous models are set up with initial slab geometries derived from tomography and realistic plate boundary configurations and incorporate the major crustal weak zones of the overriding plate. Our modeling results in predictions of flow fields and stress, strain rate and rotation rate fields for the present-day tectonic setting of the Aegean region. By comparing our various model predictions to the widely available observations, such as focal mechanisms, GPS velocities and seismic anisotropy, we aim at an improved understanding of how mantle flow, subduction morphology and possibly slab segmentation, as well as the rheological behavior of the overriding plate, control present-day tectonic deformation. We expect to show preliminary results of this comparison. Kronbichler, M., Heister, T. and Bangerth, W. (2012), High Accuracy Mantle Convection Simulation through Modern Numerical Methods, Geophysical Journal International, 191, 12-29. Van Hinsbergen, D. J. J., Hafkenscheid, E., Spakman, W., Meulenkamp, J. E. and Wortel, R. (2005

  20. Present-Day Surface Changes on Mars: Implications for Recent Climate Variability and Habitability

    NASA Astrophysics Data System (ADS)

    McEwen, A. S.; Dundas, C. M.; Diniega, S.; Byrne, S.; Bridges, N. T.; Hansen, C. J.

    2012-12-01

    With the high-resolution and repeat-image capability of MRO/HiRISE, we have been documenting present-day surface activity. This activity includes seasonal defrosting (spots, fans, etc.), changes in polar deposits, new impacts, migrating sand dunes, enlargement of gullies, and a variety of slope flows. What does this tell us about possible environmental change and habitability? Perhaps the key result is that previous suggestions of recent climate change on Mars may have been somewhat exaggerated. One such suggestion is that the enlargement of pits in the south polar residual cap indicates present-day global warming. However, recent models of continuous sublimation and redeposition of the CO2 predict a suite of landforms that have been observed to exist today (Byrne, 2009, AREPS 37, 535). Another suggestion is that mid-latitude gullies formed by melting snow or shallow ice after a recent period of high obliquity, but HiRISE observations have shown rapid and widespread gully activity in the present climate (Diniega et al., 2010, Geology 38, 1047; Dundas et al., 2012, Icarus 220, 124; Dundas et al., this conference). Likewise, suggestions that Mars needed a significantly higher atmospheric density to explain the presence of well-preserved sand dunes have been countered by observations of widespread current activity (Bridges et al., 2012, Geology 40, 31; Bridges et al., 2012, Nature 485, 339). These observations do not rule out significantly different past climate conditions but do suggest that their effects were less pronounced, at least in recent times. There are features that do not appear active today; one example is the transverse aeolian ridges. Also, the mid-latitude icy lobate flows and ice-rich mantles have not shown current activity, appear to have partially sublimated, and are likely remnants of recent past climates. Ground ice excavated by new craters is observed closer to the equator than predicted for the present atmospheric water vapor content, but

  1. Present-day impact cratering rate and contemporary gully activity on Mars.

    PubMed

    Malin, Michael C; Edgett, Kenneth S; Posiolova, Liliya V; McColley, Shawn M; Dobrea, Eldar Z Noe

    2006-12-01

    The Mars Global Surveyor Mars Orbiter Camera has acquired data that establish the present-day impact cratering rate and document new deposits formed by downslope movement of material in mid-latitude gullies on Mars. Twenty impacts created craters 2 to 150 meters in diameter within an area of 21.5 x 10(6) square kilometers between May 1999 and March 2006. The values predicted by models that scale the lunar cratering rate to Mars are close to the observed rate, implying that surfaces devoid of craters are truly young and that as yet unrecognized processes of denudation must be operating. The new gully deposits, formed since August 1999, are light toned and exhibit attributes expected from emplacement aided by a fluid with the properties of liquid water: relatively long, extended, digitate distal and marginal branches, diversion around obstacles, and low relief. The observations suggest that liquid water flowed on the surface of Mars during the past decade.

  2. Larval therapy from antiquity to the present day: mechanisms of action, clinical applications and future potential

    PubMed Central

    Whitaker, Iain S; Twine, Christopher; Whitaker, Michael J; Welck, Mathew; Brown, Charles S; Shandall, Ahmed

    2007-01-01

    When modern medicine fails, it is often useful to draw ideas from ancient treatments. The therapeutic use of fly larvae to debride necrotic tissue, also known as larval therapy, maggot debridement therapy or biosurgery, dates back to the beginnings of civilisation. Despite repeatedly falling out of favour largely because of patient intolerance to the treatment, the practice of larval therapy is increasing around the world because of its efficacy, safety and simplicity. Clinical indications for larval treatment are varied, but, in particular, are wounds infected with multidrug‐resistant bacteria and the presence of significant co‐morbidities precluding surgical intervention. The flies most often used in larval therapy are the facultative calliphorids, with the greenbottle blowfly (Lucilia sericata) being the most widely used species. This review summarises the fascinating and turbulent history of larval therapy from its origin to the present day, including mechanisms of action and evidence for its clinical applications. It also explores future research directions. PMID:17551073

  3. Present-day impact cratering rate and contemporary gully activity on Mars.

    PubMed

    Malin, Michael C; Edgett, Kenneth S; Posiolova, Liliya V; McColley, Shawn M; Dobrea, Eldar Z Noe

    2006-12-01

    The Mars Global Surveyor Mars Orbiter Camera has acquired data that establish the present-day impact cratering rate and document new deposits formed by downslope movement of material in mid-latitude gullies on Mars. Twenty impacts created craters 2 to 150 meters in diameter within an area of 21.5 x 10(6) square kilometers between May 1999 and March 2006. The values predicted by models that scale the lunar cratering rate to Mars are close to the observed rate, implying that surfaces devoid of craters are truly young and that as yet unrecognized processes of denudation must be operating. The new gully deposits, formed since August 1999, are light toned and exhibit attributes expected from emplacement aided by a fluid with the properties of liquid water: relatively long, extended, digitate distal and marginal branches, diversion around obstacles, and low relief. The observations suggest that liquid water flowed on the surface of Mars during the past decade. PMID:17158321

  4. Modelling economic losses of historic and present-day high-impact winter storms in Switzerland

    NASA Astrophysics Data System (ADS)

    Welker, Christoph; Martius, Olivia; Stucki, Peter; Bresch, David; Dierer, Silke; Brönnimann, Stefan

    2015-04-01

    Windstorms can cause significant financial damage and they rank among the most hazardous meteorological hazards in Switzerland. Risk associated with windstorms involves the combination of hazardous weather conditions, such as high wind gust speeds, and socio-economic factors, such as the distribution of assets as well as their susceptibilities to damage. A sophisticated risk assessment is important in a wide range of areas and has benefits for e.g. the insurance industry. However, a sophisticated risk assessment needs a large sample of storm events for which high-resolution, quantitative meteorological and/or loss data are available. Latter is typically an aggravating factor. For present-day windstorms in Switzerland, the data basis is generally sufficient to describe the meteorological development and wind forces as well as the associated impacts. In contrast, historic windstorms are usually described by graphical depictions of the event and/or by weather and loss reports. The information on historic weather events is overall sparse and the available historic weather and loss reports mostly do not provide quantitative information. It has primarily been the field of activity of environmental historians to study historic weather extremes and their impacts. Furthermore, the scarce availability of atmospheric datasets reaching back sufficiently in time has so far limited the analysis of historic weather events. The Twentieth Century Reanalysis (20CR) ensemble dataset, a global atmospheric reanalysis currently spanning 1871 to 2012, offers potentially a very valuable resource for the analysis of historic weather events. However, the 2°×2° latitude-longitude grid of the 20CR is too coarse to realistically represent the complex orography of Switzerland, which has considerable ramifications for the representation of smaller-scale features of the surface wind field influenced by the local orography. Using the 20CR as a starting point, this study illustrates a method to

  5. Ancient human genomes suggest three ancestral populations for present-day Europeans.

    PubMed

    Lazaridis, Iosif; Patterson, Nick; Mittnik, Alissa; Renaud, Gabriel; Mallick, Swapan; Kirsanow, Karola; Sudmant, Peter H; Schraiber, Joshua G; Castellano, Sergi; Lipson, Mark; Berger, Bonnie; Economou, Christos; Bollongino, Ruth; Fu, Qiaomei; Bos, Kirsten I; Nordenfelt, Susanne; Li, Heng; de Filippo, Cesare; Prüfer, Kay; Sawyer, Susanna; Posth, Cosimo; Haak, Wolfgang; Hallgren, Fredrik; Fornander, Elin; Rohland, Nadin; Delsate, Dominique; Francken, Michael; Guinet, Jean-Michel; Wahl, Joachim; Ayodo, George; Babiker, Hamza A; Bailliet, Graciela; Balanovska, Elena; Balanovsky, Oleg; Barrantes, Ramiro; Bedoya, Gabriel; Ben-Ami, Haim; Bene, Judit; Berrada, Fouad; Bravi, Claudio M; Brisighelli, Francesca; Busby, George B J; Cali, Francesco; Churnosov, Mikhail; Cole, David E C; Corach, Daniel; Damba, Larissa; van Driem, George; Dryomov, Stanislav; Dugoujon, Jean-Michel; Fedorova, Sardana A; Gallego Romero, Irene; Gubina, Marina; Hammer, Michael; Henn, Brenna M; Hervig, Tor; Hodoglugil, Ugur; Jha, Aashish R; Karachanak-Yankova, Sena; Khusainova, Rita; Khusnutdinova, Elza; Kittles, Rick; Kivisild, Toomas; Klitz, William; Kučinskas, Vaidutis; Kushniarevich, Alena; Laredj, Leila; Litvinov, Sergey; Loukidis, Theologos; Mahley, Robert W; Melegh, Béla; Metspalu, Ene; Molina, Julio; Mountain, Joanna; Näkkäläjärvi, Klemetti; Nesheva, Desislava; Nyambo, Thomas; Osipova, Ludmila; Parik, Jüri; Platonov, Fedor; Posukh, Olga; Romano, Valentino; Rothhammer, Francisco; Rudan, Igor; Ruizbakiev, Ruslan; Sahakyan, Hovhannes; Sajantila, Antti; Salas, Antonio; Starikovskaya, Elena B; Tarekegn, Ayele; Toncheva, Draga; Turdikulova, Shahlo; Uktveryte, Ingrida; Utevska, Olga; Vasquez, René; Villena, Mercedes; Voevoda, Mikhail; Winkler, Cheryl A; Yepiskoposyan, Levon; Zalloua, Pierre; Zemunik, Tatijana; Cooper, Alan; Capelli, Cristian; Thomas, Mark G; Ruiz-Linares, Andres; Tishkoff, Sarah A; Singh, Lalji; Thangaraj, Kumarasamy; Villems, Richard; Comas, David; Sukernik, Rem; Metspalu, Mait; Meyer, Matthias; Eichler, Evan E; Burger, Joachim; Slatkin, Montgomery; Pääbo, Svante; Kelso, Janet; Reich, David; Krause, Johannes

    2014-09-18

    We sequenced the genomes of a ∼7,000-year-old farmer from Germany and eight ∼8,000-year-old hunter-gatherers from Luxembourg and Sweden. We analysed these and other ancient genomes with 2,345 contemporary humans to show that most present-day Europeans derive from at least three highly differentiated populations: west European hunter-gatherers, who contributed ancestry to all Europeans but not to Near Easterners; ancient north Eurasians related to Upper Palaeolithic Siberians, who contributed to both Europeans and Near Easterners; and early European farmers, who were mainly of Near Eastern origin but also harboured west European hunter-gatherer related ancestry. We model these populations' deep relationships and show that early European farmers had ∼44% ancestry from a 'basal Eurasian' population that split before the diversification of other non-African lineages.

  6. Ancient human genomes suggest three ancestral populations for present-day Europeans.

    PubMed

    Lazaridis, Iosif; Patterson, Nick; Mittnik, Alissa; Renaud, Gabriel; Mallick, Swapan; Kirsanow, Karola; Sudmant, Peter H; Schraiber, Joshua G; Castellano, Sergi; Lipson, Mark; Berger, Bonnie; Economou, Christos; Bollongino, Ruth; Fu, Qiaomei; Bos, Kirsten I; Nordenfelt, Susanne; Li, Heng; de Filippo, Cesare; Prüfer, Kay; Sawyer, Susanna; Posth, Cosimo; Haak, Wolfgang; Hallgren, Fredrik; Fornander, Elin; Rohland, Nadin; Delsate, Dominique; Francken, Michael; Guinet, Jean-Michel; Wahl, Joachim; Ayodo, George; Babiker, Hamza A; Bailliet, Graciela; Balanovska, Elena; Balanovsky, Oleg; Barrantes, Ramiro; Bedoya, Gabriel; Ben-Ami, Haim; Bene, Judit; Berrada, Fouad; Bravi, Claudio M; Brisighelli, Francesca; Busby, George B J; Cali, Francesco; Churnosov, Mikhail; Cole, David E C; Corach, Daniel; Damba, Larissa; van Driem, George; Dryomov, Stanislav; Dugoujon, Jean-Michel; Fedorova, Sardana A; Gallego Romero, Irene; Gubina, Marina; Hammer, Michael; Henn, Brenna M; Hervig, Tor; Hodoglugil, Ugur; Jha, Aashish R; Karachanak-Yankova, Sena; Khusainova, Rita; Khusnutdinova, Elza; Kittles, Rick; Kivisild, Toomas; Klitz, William; Kučinskas, Vaidutis; Kushniarevich, Alena; Laredj, Leila; Litvinov, Sergey; Loukidis, Theologos; Mahley, Robert W; Melegh, Béla; Metspalu, Ene; Molina, Julio; Mountain, Joanna; Näkkäläjärvi, Klemetti; Nesheva, Desislava; Nyambo, Thomas; Osipova, Ludmila; Parik, Jüri; Platonov, Fedor; Posukh, Olga; Romano, Valentino; Rothhammer, Francisco; Rudan, Igor; Ruizbakiev, Ruslan; Sahakyan, Hovhannes; Sajantila, Antti; Salas, Antonio; Starikovskaya, Elena B; Tarekegn, Ayele; Toncheva, Draga; Turdikulova, Shahlo; Uktveryte, Ingrida; Utevska, Olga; Vasquez, René; Villena, Mercedes; Voevoda, Mikhail; Winkler, Cheryl A; Yepiskoposyan, Levon; Zalloua, Pierre; Zemunik, Tatijana; Cooper, Alan; Capelli, Cristian; Thomas, Mark G; Ruiz-Linares, Andres; Tishkoff, Sarah A; Singh, Lalji; Thangaraj, Kumarasamy; Villems, Richard; Comas, David; Sukernik, Rem; Metspalu, Mait; Meyer, Matthias; Eichler, Evan E; Burger, Joachim; Slatkin, Montgomery; Pääbo, Svante; Kelso, Janet; Reich, David; Krause, Johannes

    2014-09-18

    We sequenced the genomes of a ∼7,000-year-old farmer from Germany and eight ∼8,000-year-old hunter-gatherers from Luxembourg and Sweden. We analysed these and other ancient genomes with 2,345 contemporary humans to show that most present-day Europeans derive from at least three highly differentiated populations: west European hunter-gatherers, who contributed ancestry to all Europeans but not to Near Easterners; ancient north Eurasians related to Upper Palaeolithic Siberians, who contributed to both Europeans and Near Easterners; and early European farmers, who were mainly of Near Eastern origin but also harboured west European hunter-gatherer related ancestry. We model these populations' deep relationships and show that early European farmers had ∼44% ancestry from a 'basal Eurasian' population that split before the diversification of other non-African lineages. PMID:25230663

  7. The Combined Landscape of Denisovan and Neanderthal Ancestry in Present-Day Humans.

    PubMed

    Sankararaman, Sriram; Mallick, Swapan; Patterson, Nick; Reich, David

    2016-05-01

    Some present-day humans derive up to ∼5% [1] of their ancestry from archaic Denisovans, an even larger proportion than the ∼2% from Neanderthals [2]. We developed methods that can disambiguate the locations of segments of Denisovan and Neanderthal ancestry in present-day humans and applied them to 257 high-coverage genomes from 120 diverse populations, among which were 20 individual Oceanians with high Denisovan ancestry [3]. In Oceanians, the average size of Denisovan fragments is larger than Neanderthal fragments, implying a more recent average date of Denisovan admixture in the history of these populations (p = 0.00004). We document more Denisovan ancestry in South Asia than is expected based on existing models of history, reflecting a previously undocumented mixture related to archaic humans (p = 0.0013). Denisovan ancestry, just like Neanderthal ancestry, has been deleterious on a modern human genetic background, as reflected by its depletion near genes. Finally, the reduction of both archaic ancestries is especially pronounced on chromosome X and near genes more highly expressed in testes than other tissues (p = 1.2 × 10(-7) to 3.2 × 10(-7) for Denisovan and 2.2 × 10(-3) to 2.9 × 10(-3) for Neanderthal ancestry even after controlling for differences in level of selective constraint across gene classes). This suggests that reduced male fertility may be a general feature of mixtures of human populations diverged by >500,000 years.

  8. The Combined Landscape of Denisovan and Neanderthal Ancestry in Present-Day Humans.

    PubMed

    Sankararaman, Sriram; Mallick, Swapan; Patterson, Nick; Reich, David

    2016-05-01

    Some present-day humans derive up to ∼5% [1] of their ancestry from archaic Denisovans, an even larger proportion than the ∼2% from Neanderthals [2]. We developed methods that can disambiguate the locations of segments of Denisovan and Neanderthal ancestry in present-day humans and applied them to 257 high-coverage genomes from 120 diverse populations, among which were 20 individual Oceanians with high Denisovan ancestry [3]. In Oceanians, the average size of Denisovan fragments is larger than Neanderthal fragments, implying a more recent average date of Denisovan admixture in the history of these populations (p = 0.00004). We document more Denisovan ancestry in South Asia than is expected based on existing models of history, reflecting a previously undocumented mixture related to archaic humans (p = 0.0013). Denisovan ancestry, just like Neanderthal ancestry, has been deleterious on a modern human genetic background, as reflected by its depletion near genes. Finally, the reduction of both archaic ancestries is especially pronounced on chromosome X and near genes more highly expressed in testes than other tissues (p = 1.2 × 10(-7) to 3.2 × 10(-7) for Denisovan and 2.2 × 10(-3) to 2.9 × 10(-3) for Neanderthal ancestry even after controlling for differences in level of selective constraint across gene classes). This suggests that reduced male fertility may be a general feature of mixtures of human populations diverged by >500,000 years. PMID:27032491

  9. How large are present-day heat flow variations across Mars' surface?

    NASA Astrophysics Data System (ADS)

    Plesa, Ana-Catalina; Tosi, Nicola; Grott, Matthias; Breuer, Doris

    2015-04-01

    The upcoming InSight (Interior exploration using Seismic Investigations, Geodesy and Heat Transport) mission, to be launched in 2016, will carry the first in-situ Martian heat flow measurement and provide an important baseline to constrain the present-day heat budget of the planet and, in turn, the thermal and chemical evolution of its interior. Currently, the Earth and the Moon are the only bodies on which in-situ surface heat flow measurements have been performed. Here, strong spatial variations of the surface heat flow are primarily caused by plate tectonics and the heterogeneous distribution of heat producing elements over the surface (e.g., the so-called Procellarum KREEP Terrane PKT on the lunar nearside). In the absence of plate-tectonics and large-scale geochemical anomalies, on Mars, surface heat flow is expected to vary less with geological location, being mainly influenced by variations in the thickness and HPE content of the crust [1], and by mantle plumes [2]. We have tested this assumption by running thermal evolution models for Mars in 3D spherical geometry, using the mantle convection code Gaia [3]. In our calculations, we employ a crust of fixed thickness with a north-south dichotomy in crustal thickness, a low conductivity compared to the mantle and enriched in radiogenic heat producing elements. Our results show that including compressibility effects, phase transitions and different core sizes, surface heat flow variations are mainly dominated by the crust contribution, unless the mantle viscosity increases more than three orders of magnitude with depth. In the latter case, heat flow variations due to mantle upwellings are ~ 8 mW/m2 relative to surface average and remain confined to limited surface regions. Both surface heat flow variations on Mars obtained from numerical models and the heat flow measurement planned for the InSight mission will permit to address the question of a possible plume underneath Elysium and also to test the feasibility

  10. Surface deformation induced by present-day ice melting in Svalbard

    NASA Astrophysics Data System (ADS)

    Kierulf, H. P.; Plag, H.-P.; Kohler, J.

    2009-10-01

    The vertical movement of the Earth's surface is the result of a number of internal processes in the solid Earth, tidal forces and mass redistribution in the atmosphere, oceans, terrestrial hydrosphere and cryosphere. Close to ice sheets and glaciers, the changes in the ice loads can induce large vertical motions at intraseasonal to secular timescales. The Global Positioning System (GPS) and Very Long Baseline Interferometry (VLBI) antennas in Ny-Ålesund, Svalbard that started observations in 1991 and 1995, respectively, observe vertical uplift rates on the order of 8 +/- 2 mmyr-1, which are considerably larger than those predicted by postglacial rebound (PGR) models (order 2 mmyr-1). The observations also indicate increased uplift rates starting some time in 2000. A local GPS campaign network that has been reoccupied annually since 1998, reveals a tilting away from the neighbouring glaciers. The Svalbard glaciers have been undergoing melting and retreat during the last century, with increased melting since about 2000. We compared the observed vertical motion to the motion predicted by loading models using a detailed ice model with annual time resolution as forcing. The model predictions correlate well with the observations both with respect to the interannual variations and the spatial pattern of long-term trends. The regression coefficients for predicted and observed interannual variations in height is 1.08 +/- 0.38, whereas the regression coefficient for the predicted and observed spatial pattern turns out to be 1.26 +/- 0.42. Estimates of the predicted secular trend in height due to PGR and present-day melting are on the order of 4.8 +/- 0.3 mmyr-1 and thus smaller than the observed secular trend in height. This discrepancy between predictions and observations is likely caused by the sum of errors in the secular rates determined from observations (due to technique-dependent large-scale offsets) and incomplete or erroneous models (unaccounted tectonic vertical

  11. Simulation of Net Infiltration for Present-Day and Potential Future Climates

    SciTech Connect

    D. Levitt

    2004-11-09

    The purpose of this model report is to document the infiltration model used to estimate upper-bound, mean, and lower-bound spatially-distributed average annual net infiltration rates for present-day and potential future climates at Yucca Mountain, Nevada. Net infiltration is the component of infiltrated precipitation, snowmelt, or surface water run-on that has percolated below the zone of evapotranspiration as defined by the depth of the effective root zone. The estimates of net infiltration are primarily used for defining the upper boundary condition for the site-scale three-dimensional unsaturated zone (UZ) model. The UZ flow model is one of several process models abstracted by the total system performance assessment (TSPA) model used to evaluate performance of the repository at Yucca Mountain, Nevada. The net-infiltration model is important for assessing repository-system performance because output from this model provides the upper boundary condition for the UZ flow model used to generate flow fields; water percolating downward from the UZ will be the principal means by which radionuclides are potentially released to the saturated zone (SZ). The SZ is the principal pathway to the biosphere where the reasonably maximally exposed individual (RMEI) is exposed to radionuclides.

  12. Present-day surface deformation and tectonic insights of the extensional Ilan Plain, NE Taiwan

    NASA Astrophysics Data System (ADS)

    Kang, Chu-Chun; Chang, Chung-Pai; Siame, Lionel; Lee, Jian-Cheng

    2015-06-01

    Taiwan's mountain belt is an ideal location to address major questions regarding mechanisms of lithospheric deformation in convergent settings, mountain building processes from oceanic subduction to continental subduction, and post orogenic extension. In the northeast of this belt, the Ilan Plain is a triangular, deltaic plain characterized by a flat topography close to the sea level, and surrounded by the high mountains of the Hsuehshan Range to the northwest, and the Central Range to the southeast. Its eastern coast faces the western tip of the Okinawa Trough, the back-arc basin of the Ryukyu subduction zone. In this study, we analyzed the present-day surface deformation of the Ilan Plain, aiming at deciphering its relationships with basement faults and the regional geodynamic setting. Our approach is mainly based on surface vertical displacements revealed by Persistent Scatterer Interferometry Synthetic Aperture Radar (PSI), which indicate that there is an area of active subsidence (∼18 mm/yr) located in the southern part of the plain in probable connection with active basement faults and in agreement with previous geodetic measurements and existing geophysical data. Our PSI results also suggest that the subsidence occurring in the Ilan Plain has moved from north to south during Quaternary in relation with extrusion of the belt due to the westward propagation of the Okinawa Trough through the Taiwan Mountains.

  13. Present-day deformation of northern Pakistan from Salt Ranges to Karakorum Ranges

    NASA Astrophysics Data System (ADS)

    Jouanne, F.; Awan, A.; Pêcher, A.; Kausar, A.; Mugnier, J. L.; Khan, I.; Khan, N. A.; Van Melle, J.

    2014-03-01

    Episodic GPS measurements are used to quantify the present-day velocity field in the northwestern Himalaya from the southern Pamir to the Himalayan foreland. We report large postseismic displacements following the 2005 Kashmir earthquake and several mm/yr thrusting of the central segment of the Salt Ranges and Potwar Plateau over the foreland, westward thrusting of Nanga Parbat above the Kohistan Plateau, and ~12 mm/yr SSE velocities of the Karakorum Ranges and of the Deosai and Kohistan Plateaus relative to the Indian Plate. Numerical simulations allow to determine a first approximation of slip along active faults: (1) substantial creep of ~87 mm/yr between 2006 and 2012 along the flat northeast of the Balakot-Bagh Thrust affected by the 2005 earthquake; (2) ~5 mm/yr slip of the central segment of the Salt Ranges and Potwar Plateau, whereas their western boundaries are clearly inactive over the time span covered by our measurements; (3) 13 mm/yr ductile slip along the Main Himalayan Thrust modeled by a dislocation dipping 7° northward, locked at a depth of 15 km; and (4) ~20 mm/yr slip along the shear zone forming the western boundary of Nanga Parbat, between depths of 1.6 and 6.5 km. Residuals velocities suggest the existence of left-lateral strike slip along the Jhelum Fault.

  14. The history of time and frequency from antiquity to the present day

    NASA Astrophysics Data System (ADS)

    Levine, Judah

    2016-04-01

    I will discuss the evolution of the definitions of time, time interval, and frequency from antiquity to the present day. The earliest definitions of these parameters were based on a time interval defined by widely observed apparent astronomical phenomena, so that techniques of time distribution were not necessary. With this definition, both time, as measured by clocks, and frequency, as realized by some device, were derived quantities. On the other hand, the fundamental parameter today is a frequency based on the properties of atoms, so that the situation is reversed and time and time interval are now derived quantities. I will discuss the evolution of this transition and its consequences. In addition, the international standards of both time and frequency are currently realized by combining the data from a large number of devices located at many different laboratories, and this combination depends on (and is often limited by) measurements of the times of clocks located at widely-separated laboratories. I will discuss how these measurements are performed and how the techniques have evolved over time.

  15. Challenges in constraining anthropogenic aerosol effects on cloud radiative forcing using present-day spatiotemporal variability.

    PubMed

    Ghan, Steven; Wang, Minghuai; Zhang, Shipeng; Ferrachat, Sylvaine; Gettelman, Andrew; Griesfeller, Jan; Kipling, Zak; Lohmann, Ulrike; Morrison, Hugh; Neubauer, David; Partridge, Daniel G; Stier, Philip; Takemura, Toshihiko; Wang, Hailong; Zhang, Kai

    2016-05-24

    A large number of processes are involved in the chain from emissions of aerosol precursor gases and primary particles to impacts on cloud radiative forcing. Those processes are manifest in a number of relationships that can be expressed as factors dlnX/dlnY driving aerosol effects on cloud radiative forcing. These factors include the relationships between cloud condensation nuclei (CCN) concentration and emissions, droplet number and CCN concentration, cloud fraction and droplet number, cloud optical depth and droplet number, and cloud radiative forcing and cloud optical depth. The relationship between cloud optical depth and droplet number can be further decomposed into the sum of two terms involving the relationship of droplet effective radius and cloud liquid water path with droplet number. These relationships can be constrained using observations of recent spatial and temporal variability of these quantities. However, we are most interested in the radiative forcing since the preindustrial era. Because few relevant measurements are available from that era, relationships from recent variability have been assumed to be applicable to the preindustrial to present-day change. Our analysis of Aerosol Comparisons between Observations and Models (AeroCom) model simulations suggests that estimates of relationships from recent variability are poor constraints on relationships from anthropogenic change for some terms, with even the sign of some relationships differing in many regions. Proxies connecting recent spatial/temporal variability to anthropogenic change, or sustained measurements in regions where emissions have changed, are needed to constrain estimates of anthropogenic aerosol impacts on cloud radiative forcing.

  16. [Psychiatry during National Socialism: historical knowledge, implications for present day ethical debates].

    PubMed

    Roelcke, V

    2010-11-01

    This contribution is a synthesis of the results of historical research on psychiatry during the Nazi period and some implications for present day debates in medical ethics. The focus is on three issues: the relationship between physicians and the state, the impact of eugenically and economically motivated health and social policies for psychiatry (e.g. forced sterilization, patient killing/euthanasia) and psychiatric research. Three myths are deconstructed: 1) that medical atrocities were imposed from above by Nazi politicians on apolitical physicians, 2) that mass sterilization and patient killing had nothing to do with contemporary state of the art of medical reasoning and practice and 3) that ethically unacceptable research on psychiatric patients had nothing to do with the contemporary state of the art of biomedical sciences. It is argued that the findings on these issues of Nazi medicine are not specific to Germany and the period between 1933 and 1945 but they were the extreme manifestations of some potential problems implicit in modern medicine in general.

  17. Present-day stress field in the Gibraltar Arc (western Mediterranean)

    NASA Astrophysics Data System (ADS)

    FernáNdez-IbáñEz, F.; Soto, J. I.; Zoback, M. D.; Morales, J.

    2007-08-01

    The Gibraltar Arc in the western Mediterranean consists of the Betic and Rif Alpine chains and the Alboran Sea Basin. Four types of stress indicators (wellbore breakouts, earthquake focal plane mechanisms, young geologic fault slip data, and hydraulic fracture orientations) indicate a regional NW-SE compressive stress field resulting from Africa-Eurasia plate convergence. In some particular regions, deviations of SHmax are observed with respect to the regional stress field. They are gentle-to-moderate (22°-36°) anticlockwise rotations located along the North Alboran margin and moderate-to-significant (36°-78°) clockwise rotations around the Trans-Alboran Shear Zone (TASZ). This is a broad fault zone composed of different left-lateral strike-slip fault segments running from the eastern Betics to the Alhoceima region in the Rif and resulting in a major bathymetric high in the Alboran Sea (the Alboran Ridge fault zone). Some of these stress rotations appear to be controlled by steep gradients of crustal thickness variation across the North Alboran margin and/or differential loading imposed by thick sedimentary accumulations in basin depocenters parallel to the shoreline. Other stress perturbations may be related to active left-lateral, strike-slip deformation within the TASZ that crosscuts the entire orogenic arc on a NE-SW trend and represents a key element to understand present-day deformation partitioning in the western Mediterranean.

  18. Differences between Last Glacial Maximum and present-day temperature and precipitation in southern South America

    NASA Astrophysics Data System (ADS)

    Berman, Ana Laura; Silvestri, Gabriel E.; Tonello, Marcela S.

    2016-10-01

    This paper is the first analysis of differences between Last Glacial Maximum (LGM) and present climates in southern South America considering the state-of-the-art PMIP3 paleoclimatic models. The study is focused on characteristics of temperature and precipitation over the portion of the continent to the south of 20°S at both sides of the Andes Cordillera. Results demonstrate that model outputs coincide with glacial conditions inferred from the very few paleorecords available in the region. Consequently, these models are a valuable tool for inferring additional conditions in areas where there is a lack of proxy information allowing the reconstruction of the past climate at regional scales. The analyzed PMIP3 models expose an LGM cooling of ∼2-5 °C throughout the year over almost all southern South America but differences are even more pronounced in areas around the southern Andes. Models also suggest that LGM precipitation was substantially lower than present over the portion of southern South America to the east of the Andes inferring reductions of ∼20-30% with respect to present-day values in subtropical areas and ∼40-50% in the southern tip of the continent.

  19. Global hotspots in the present-day distribution of ancient animal and plant lineages

    PubMed Central

    Procheş, Şerban; Ramdhani, Syd; Perera, Sandun J.; Ali, Jason R.; Gairola, Sanjay

    2015-01-01

    The current distribution of biotic lineages that emerged in the deep time has both theoretical and practical implications, in particular for understanding the processes that have forged present-day biodiversity and informing local and regional-scale conservation efforts. To date however, there has been no examination of such patterns globally across taxa and geological time. Here we map the diversity of selected extant seed plant and tetrapod vertebrate lineages that were already in existence either in the latest Triassic or latest Cretaceous. For Triassic-age linages, we find concentrations in several regions – both tropical and temperate – parts of North America, Europe, East and South-east Asia, northern South America, and New Zealand. With Cretaceous-age lineages, high values are relatively uniformly distributed across the tropics, with peak the values along the Andes, in South-east Asia and Queensland, but also in the temperate Cape Mountains. These patterns result from a combination of factors, including land area, geographic isolation, climate stability and mass extinction survival ability. While the need to protect many of these lineages has been long recognised, a spatially-explicit approach is critical for understanding and maintaining the factors responsible for their persistence, and this will need to be taken forward across finer scales. PMID:26498226

  20. Present-day climate near the Bunker Cave (Germany): comparison of model simulations to observations.

    NASA Astrophysics Data System (ADS)

    Langebroek, Petra M.; Werner, Martin; Lohmann, Gerrit

    2010-05-01

    Because of their high temporal resolution and good preservation, speleothem records are often used as indicators for past climatic conditions. During the last years, substantial scientific progress has been made on the interpretation of these proxy records in terms of temperature and precipitation changes. Unfortunately, the link between climatic conditions within the cave (as depicted from the speleothems) to the climate outside the cave is not straightforward for many cave locations. In order to improve this connection, it is important to have accurate speleothems data as well as high-resolution large-scale climate data. In this study we focus on the latter problem. Within the framework of the DAPHNE ("Dated Speleothems Archives of the Paleoenvironment") Project we investigated available climate measurements (stations from the Deutsche Wetterdienst and from the Global Network of Isotopes in Precipitation) in the region surrounding the Bunker Cave, which is located close to Iserlohn in Germany. These measurements are compared, on a larger scale, to reanalysis data (ECMWF-ERA40) and simulation results from an atmospheric general circulation model with isotope diagnostics built into the models hydrological cycle (ECHAM). We used statistical downscaling to establish a connection between the large-scale information and the local climate surrounding the Bunker Cave. Understanding how the local present-day climate above the cave is influenced will largely improve our knowledge of palaeoclimate as derived from the speleothem records.

  1. The evolution of galaxies from primeval irregulars to present-day ellipticals.

    PubMed

    Mori, Masao; Umemura, Masayuki

    2006-03-30

    Galaxy formation is believed to proceed in a 'bottom up' manner, starting with the formation of small clumps of gas and stars that then merge hierarchically into giant systems. The baryonic gas loses thermal energy by radiative cooling and falls towards the centres of the new galaxies, while supernovae blow gas out. Any realistic model therefore requires a proper treatment of these processes, but hitherto this has been far from satisfactory. Here we report a simulation that follows evolution from the earliest stages of galaxy formation through the period of dynamical relaxation, at which point the resulting galaxy is in its final form. The bubble structures of gas revealed in our simulation (for times of less than 3 x 10(8) years) resemble closely high-redshift Lyman-alpha emitters. After 10(9) years, these bodies are dominated by stellar continuum radiation and then resemble the Lyman break galaxies, which are high-redshift star-forming galaxies. At this point, the abundance of elements heavier than helium ('metallicity') appears to be solar. After 1.3 x 10(10) years, these galaxies resemble present-day ellipticals.

  2. Cannabis careers revisited: applying Howard S. Becker's theory to present-day cannabis use.

    PubMed

    Järvinen, Margaretha; Ravn, Signe

    2014-01-01

    A considerable part of today's sociological research on recreational drug use is (explicitly or implicitly) inspired by Howard Becker's classical model of deviant careers. The aim of the present paper is to directly apply Becker's theory to empirical data on present-day cannabis use and to suggest a revision of the theory. As part of this, we propose a stretch of the sociological approach represented by Becker and followers in order to include, not only recreational drug use, but also use for which young people have sought treatment. The paper is based on 30 qualitative interviews with young people in treatment for cannabis problems in Copenhagen, Denmark. We suggest a revision of Becker's career model in relation to four aspects: initiation of cannabis use, differentiation between socially integrated and individualised, disintegrated use, social control from non-users, and the users' moral stance on cannabis. A central point of the paper is that social interaction may both motivate cannabis use, as Becker proposed, and serve as a protective factor against extensive, problematic use.

  3. Recovery of a geocentric reference frame using the present-day GPS system

    NASA Technical Reports Server (NTRS)

    Malla, Rajendra P.; Wu, Sien-Chong

    1990-01-01

    A geocentric reference frame adopts the center of mass of the earth as the origin of the coordinate axes. The center of mass of the earth is the natural and unambiguous origin of a geocentric satellite dynamical system. But in practice a kinematically obtained terrestrial reference frame may assume an origin other than the geocenter. The establishment of a geocentric reference frame, to which all relevant observations and results can be referred and in which geodynamic theories or models for the dynamic behavior of earth can be formulated, requires the ability to accurately recover a given coordinate frame origin offset from the geocenter. GPS measurements, because of their abundance and broad distribution, provide a powerful tool to obtain this origin offset in a short period of time. Two effective strategies have been devised. Data from the First Central And South America (Casa Uno) geodynamics experiment has been studied, in order to demonstrate the ability of recovering the geocenter location with present day GPS satellites and receivers.

  4. The present-day climate of Greenland : a study with a regional climate model

    NASA Astrophysics Data System (ADS)

    Ettema, J.

    2010-04-01

    Present-day climate of Greenland Over the past 20 years, the Greenland ice sheet (GrIS) has warmed. This temperature increase can be explained by an increase in downwelling longwave radiation due to a warmer overlying atmosphere. These temperature changes are strongly correlated to changes in the large scale circulation over the ice sheet. Since 1990, the melt has also strongly increased along the ice margins, inducing significant increase in runoff. With no significant change found in the total precipitation, the GrIS surface mass balance (SMB) decreased by 12 Gt yr-1 or 7 kg m-2 yr-1 since 1990. Locally, the SMB trend reaches -90 kg m-2 yr-1 at the western and eastern ice margins. These conclusions are drawn from a modelling study by Janneke Ettema, which discusses the present-day climate and surface mass balance of the GrIS. The emphasis of this research is on understanding the underlying physical processes. Using the regional atmospheric climate model RACMO2/GR at high horizontal resolution (11km) has resulted in unprecedented detail in the ice sheet climatology and SMB. By incorporating processes such as percolation, retention and refreezing of meltwater in the surface parameterisation, the model explicitly calculates how these processes affect snow pack temperature, density and surface albedo. RACMO2/GR shows that the GrIS climate is spatially very variable. Characteristic for the ice sheet climate are the persistent katabatic winds and a quasi-permanent surface temperature deficit. Due to strong radiative cooling and turbulent heat transport towards the surface, the atmospheric boundary layer cools, providing optimal conditions for strong katabatic winds to occur. The strongest temperature deficit and wind speeds are found in the northeastern part of the ice sheet, whereas in the lower ablation zone the temperatures are more moderate due to surface melt and warm air advection. The high-resolution climate model revealed that the surface mass balance of the Gr

  5. Relationship between the present-day stress field and plate boundary forces in the Pacific Northwest

    USGS Publications Warehouse

    Geist, E.L.

    1996-01-01

    The relationship between plate boundary forces and the observed stress field in the Pacific Northwest is established using numerical models of continental deformation. Because the orientation of the greatest horizontal principal stress throughout the Pacific Northwest differs considerably from the direction of convergence between the Juan de Fuca and North American plates, the relationship between the stress field and forces acting along the subduction zone has been unclear. To address this relationship, a two-dimensional finite element model developed by Bird [1989] is used that incorporates critical aspects of continental deformation such as a stratified rheology and interaction between thermal and mechanical components of deformation. Boundary conditions are specified in terms of either velocity or shear traction, depending on whether the computed shear stress at the plate boundary is less than or exceeds, respectively, a prescribed limit. Shear-stress limits on the subduction and transform plate boundaries are independently varied to determine the relative effect of forces along these boundaries on intraplate deformation. Results from this study indicate that the shear stress limit of both subduction and transform boundaries is low, and that the intraplate stress field is attributed, in part, to the normal component of relative plate motion along the transform boundaries. However, the models also indicate that although the subduction zone fault is weak, a minimum shear strength ( ??? 10 MPa) for the fault is necessary to explain the observed stress field. The balance among forces along the tectonic boundaries of North America results in a surprising degree of variation in the present-day stress field.

  6. Global Source-Receptor Relationships for Mercury Deposition Under Present-Day and 2050 Emissions Scenarios

    PubMed Central

    Corbitt, Elizabeth S.; Jacob, Daniel J.; Holmes, Christopher D.; Streets, David G.; Sunderland, Elsie M.

    2011-01-01

    Global policies regulating anthropogenic mercury require an understanding of the relationship between emitted and deposited mercury on intercontinental scales. Here we examine source-receptor relationships for present-day conditions and for four 2050 IPCC scenarios encompassing a range of economic development and environmental regulation projections. We use the GEOS-Chem global model to track mercury from its point of emission through rapid cycling in surface ocean and land reservoirs to its accumulation in longer-lived ocean and soil pools. Deposited mercury has a local component (emitted HgII, lifetime of 3.7 days against deposition) and a global component (emitted Hg0, lifetime of 6 months against deposition). Fast recycling of deposited mercury through photoreduction of HgII and re-emission of Hg0 from surface reservoirs (ice, land, surface ocean) increases the effective lifetime of anthropogenic mercury to 9 months against loss to legacy reservoirs (soil pools and the subsurface ocean). This lifetime is still sufficiently short that source-receptor relationships have a strong hemispheric signature. Asian emissions are the largest source of anthropogenic deposition to all ocean basins, though there is also regional source influence from upwind continents. Current anthropogenic emissions account for only about one-third of mercury deposition to the global ocean with the remainder from natural and legacy sources. However, controls on anthropogenic emissions would have the added benefit of reducing the legacy mercury re-emitted to the atmosphere. Better understanding is needed of the timescales for transfer of mercury from active pools to stable geochemical reservoirs. PMID:22050654

  7. LAB - Transition between Fossil and Present-Day Flow-Related Seismic Anisotropy

    NASA Astrophysics Data System (ADS)

    Plomerova, J.; Babuska, V.; Vecsey, L.; Munzarova, H.

    2015-12-01

    Topography of the lithosphere-asthenosphere boundary (LAB) and structure of the continental lithosphere record geodynamic development of the outer parts of the Earth. Studying the architecture of plates and mapping the LAB discontinuity relief can help to answer questions how and when the plates assembled and to what extent they were later deformed. Differences in changes of physical properties across the "discontinuities" are significant, but sharpness of the transitions depends on optics we are using. Moreover, the nature of the boundary/transition remains uncertain. Fundamental difference in origin and orientation of seismic anisotropy in the mantle lithosphere and in the sub-lithospheric mantle has led us to develop an original approach of LAB modelling. We associate the LAB with a transition of fossil anisotropy in the rigid high-velocity lithosphere to present-day related anisotropy in the more viscous underlying asthenosphere. We present a global LAB model calculated from depth-dependences of azimuthal and radial anisotropy of surface waves (Plomerova et al., 2002) and a uniform model of the European LAB (Plomerova and Babuska, 2010), calculated from P-wave travel times collected during regional passive experiments. In the latter, the LAB is modelled according to an empirical residual-depth relation. The high velocity contrast across the LAB (δvP ~ 0.6 km/s) required by the empirical gradient cannot be accomplished by isotropic velocities of materials forming the upper mantle, while it can be explained by different orientations of the high-velocities above and below the LAB. Relief of the lower plate boundary can vary considerably on short lateral distances and it is exposed to erosion from asthenosphere. On the other hand, dipping fabrics of often sharply bounded lithospheric domains, created probably during a plate formation, indicate stability of such a fossilized structure, which alters only near the domain boundaries.

  8. A test of present-day plate geometries for northeast Asia and Japan

    NASA Technical Reports Server (NTRS)

    Demets, Charles

    1992-01-01

    Alternative geometries for the present-day configuration of plate boundaries in northeast Asia and Japan are tested using NUVEL-1 and 256 horizontal earthquake slip vectors from the Japan and northern Kuril trenches. Statistical analysis of the slip vectors is used to determine whether the North American, Eurasian, or Okhotsk plate overlies the trench. Along the northern Kuril trench, slip vectors are well-fit by the NUVEL-1 Pacific-North America Euler pole, but are poorly fit by the Pacific-Eurasia Euler pole. Results for the Japan trench are less conclusive, but suggest that much of Honshu and Hokkaido are also part of the North American plate. The simplest geometry consistent with the trench slip vectors is a geometry in which the North American plate extends south to 41 deg N, and possibly includes northern Honshu and southern Hokkaido. Although these results imply that the diffuse seismicity that connects the Lena River delta to Sakhalin Island and the eastern Sea of Japan records motion between Eurasia and North America, onshore geologic and seismic data define an additional belt of seismicity in Siberia that cannot be explained with this geometry. Assuming that these two seismic belts constitute evidence for an Okhotsk block, two published kinematic models for motion of the Okhotsk block are tested. The first model, which predicts motion of up to 15 mm/yr relative to North America, is rejected because Kuril and Japan trench slip vectors are fit more poorly than for the simpler geometry described above. The second model gives a good fit to the trench slip vectors, but only if Okhotsk-North America motion is slower than 5 mm/yr.

  9. Challenges in constraining anthropogenic aerosol effects on cloud radiative forcing using present-day spatiotemporal variability

    PubMed Central

    Ghan, Steven; Wang, Minghuai; Zhang, Shipeng; Ferrachat, Sylvaine; Gettelman, Andrew; Griesfeller, Jan; Kipling, Zak; Lohmann, Ulrike; Morrison, Hugh; Neubauer, David; Partridge, Daniel G.; Stier, Philip; Takemura, Toshihiko; Wang, Hailong; Zhang, Kai

    2016-01-01

    A large number of processes are involved in the chain from emissions of aerosol precursor gases and primary particles to impacts on cloud radiative forcing. Those processes are manifest in a number of relationships that can be expressed as factors dlnX/dlnY driving aerosol effects on cloud radiative forcing. These factors include the relationships between cloud condensation nuclei (CCN) concentration and emissions, droplet number and CCN concentration, cloud fraction and droplet number, cloud optical depth and droplet number, and cloud radiative forcing and cloud optical depth. The relationship between cloud optical depth and droplet number can be further decomposed into the sum of two terms involving the relationship of droplet effective radius and cloud liquid water path with droplet number. These relationships can be constrained using observations of recent spatial and temporal variability of these quantities. However, we are most interested in the radiative forcing since the preindustrial era. Because few relevant measurements are available from that era, relationships from recent variability have been assumed to be applicable to the preindustrial to present-day change. Our analysis of Aerosol Comparisons between Observations and Models (AeroCom) model simulations suggests that estimates of relationships from recent variability are poor constraints on relationships from anthropogenic change for some terms, with even the sign of some relationships differing in many regions. Proxies connecting recent spatial/temporal variability to anthropogenic change, or sustained measurements in regions where emissions have changed, are needed to constrain estimates of anthropogenic aerosol impacts on cloud radiative forcing. PMID:26921324

  10. The present-day stress field orientation in Italy: new release

    NASA Astrophysics Data System (ADS)

    Montone, P.; Mariucci, M. T.

    2015-12-01

    When contemporary stress field in a region is well-known and faults are identified, it is possible to determine which faults are favorably oriented and are more likely to slip in the future. Stress data are also an important input in integrated crustal modeling to get more reliable evaluations in many applicative researches. Then, the knowledge of the active stress field contributes to the seismotectonic zoning of a region. In Italy, although at large scale a first order stress field due to plate boundary forces controls the contemporary tectonics, some areas show changes in stress regime over small distances and/or with depth clearly linked to localized stress perturbations. Where information is lacking each prediction of stress patterns could significantly differ from the reality and any further evaluation would be weakly supported by data. Therefore we continuously collect the available stress indicators and here present an update of present-day stress orientations in Italy with the last 5 years data, relative to crustal earthquake focal mechanisms (0-40 km depth), borehole breakouts from deep wells and fault data. About 100 new quality-ranked entries complete the definition of the horizontal stress orientation and tectonic regime in some areas, and bring new information mainly in Po Plain and Calabria area, recently affected by important earthquake sequences. Now the global Italian dataset consists of ~800 data points, including ~580 of A-C quality, with an increase of 17% compared to the previous compilation (Montone et al., 2012). We use A-, B- and C-quality stress indicators for analyzing first-order stress patterns while we also consider D-quality data to define second-or third-order stress field, as observed in other studies in the world. In particular we discuss the simultaneous occurrence of different stress regimes and the complex interaction between first order stress field and local effects, and the influence of the inherited tectonic structures.

  11. Uncovering the genetic history of the present-day Greenlandic population.

    PubMed

    Moltke, Ida; Fumagalli, Matteo; Korneliussen, Thorfinn S; Crawford, Jacob E; Bjerregaard, Peter; Jørgensen, Marit E; Grarup, Niels; Gulløv, Hans Christian; Linneberg, Allan; Pedersen, Oluf; Hansen, Torben; Nielsen, Rasmus; Albrechtsen, Anders

    2015-01-01

    Because of past limitations in samples and genotyping technologies, important questions about the history of the present-day Greenlandic population remain unanswered. In an effort to answer these questions and in general investigate the genetic history of the Greenlandic population, we analyzed ∼200,000 SNPs from more than 10% of the adult Greenlandic population (n = 4,674). We found that recent gene flow from Europe has had a substantial impact on the population: more than 80% of the Greenlanders have some European ancestry (on average ∼25% of their genome). However, we also found that the amount of recent European gene flow varies across Greenland and is far smaller in the more historically isolated areas in the north and east and in the small villages in the south. Furthermore, we found that there is substantial population structure in the Inuit genetic component of the Greenlanders and that individuals from the east, west, and north can be distinguished from each other. Moreover, the genetic differences in the Inuit ancestry are consistent with a single colonization wave of the island from north to west to south to east. Although it has been speculated that there has been historical admixture between the Norse Vikings who lived in Greenland for a limited period ∼600-1,000 years ago and the Inuit, we found no evidence supporting this hypothesis. Similarly, we found no evidence supporting a previously hypothesized admixture event between the Inuit in East Greenland and the Dorset people, who lived in Greenland before the Inuit.

  12. Geochemical evaluation of present-day Tuul River sediments, Ulaanbaatar basin, Mongolia.

    PubMed

    Dalai, Banzragch; Ishiga, Hiroaki

    2013-03-01

    The Tuul River flows through the Ulaanbaatar basin of Mongolia and is the main source of water for the capital city, Ulaanbaatar. The Tuul catchment can be divided into three parts around Ulaanbaatar (upper, middle, and lower), according to the extent of urbanization. Sixteen surface water and groundwater samples were collected to evaluate present-day water quality and 34 stream sediment samples taken to examine their geochemical composition in relation to provenance and to assess the impact of urban activity on heavy metal accumulation. Groundwater quality in the upper and central water sources was adequate, but high concentrations of NO (3) (-) were found in the lower water source. Heavy metal concentrations in the sediments are evaluated by comparison with average upper continental crust (UCC) values, coupled with ecological risk assessment by reference to sediment quality guidelines (SQG). The results show average abundances of potentially toxic metals such as As, Pb, Zn, Cu, Ni, Cr, and V are higher in the middle part (within the city) than in the upper and lower parts. However, all three parts show depletion in some chalcophile and high field strength elements (Cu, Ni, Cr, Sr, Nb, Zr, Th, Sc) relative to UCC, indicating that the river sediments were derived from a highly felsic crustal source. The assessment using SQG shows As and Cr are present in levels that cause adverse aquatic biological effects. Although concentrations of Pb, Zn, Cu, and Ni are generally below their respective threshold effect levels, in the middle reaches, values increase and border on the probable effect level. This suggests significant anthropogenic contamination in the urban areas, increasing values above a naturally low regional background.

  13. Challenges in constraining anthropogenic aerosol effects on cloud radiative forcing using present-day spatiotemporal variability.

    PubMed

    Ghan, Steven; Wang, Minghuai; Zhang, Shipeng; Ferrachat, Sylvaine; Gettelman, Andrew; Griesfeller, Jan; Kipling, Zak; Lohmann, Ulrike; Morrison, Hugh; Neubauer, David; Partridge, Daniel G; Stier, Philip; Takemura, Toshihiko; Wang, Hailong; Zhang, Kai

    2016-05-24

    A large number of processes are involved in the chain from emissions of aerosol precursor gases and primary particles to impacts on cloud radiative forcing. Those processes are manifest in a number of relationships that can be expressed as factors dlnX/dlnY driving aerosol effects on cloud radiative forcing. These factors include the relationships between cloud condensation nuclei (CCN) concentration and emissions, droplet number and CCN concentration, cloud fraction and droplet number, cloud optical depth and droplet number, and cloud radiative forcing and cloud optical depth. The relationship between cloud optical depth and droplet number can be further decomposed into the sum of two terms involving the relationship of droplet effective radius and cloud liquid water path with droplet number. These relationships can be constrained using observations of recent spatial and temporal variability of these quantities. However, we are most interested in the radiative forcing since the preindustrial era. Because few relevant measurements are available from that era, relationships from recent variability have been assumed to be applicable to the preindustrial to present-day change. Our analysis of Aerosol Comparisons between Observations and Models (AeroCom) model simulations suggests that estimates of relationships from recent variability are poor constraints on relationships from anthropogenic change for some terms, with even the sign of some relationships differing in many regions. Proxies connecting recent spatial/temporal variability to anthropogenic change, or sustained measurements in regions where emissions have changed, are needed to constrain estimates of anthropogenic aerosol impacts on cloud radiative forcing. PMID:26921324

  14. Far-infrared and dust properties of present-day galaxies in the EAGLE simulations

    NASA Astrophysics Data System (ADS)

    Camps, Peter; Trayford, James W.; Baes, Maarten; Theuns, Tom; Schaller, Matthieu; Schaye, Joop

    2016-10-01

    The Evolution and Assembly of GaLaxies and their Environments (EAGLE) cosmological simulations reproduce the observed galaxy stellar mass function and many galaxy properties. In this work, we study the dust-related properties of present-day EAGLE galaxies through mock observations in the far-infrared and submm wavelength ranges obtained with the 3D dust radiative transfer code SKIRT. To prepare an EAGLE galaxy for radiative transfer processing, we derive a diffuse dust distribution from the gas particles and we re-sample the star-forming gas particles and the youngest star particles into star-forming regions that are assigned dedicated emission templates. We select a set of redshift-zero EAGLE galaxies that matches the K-band luminosity distribution of the galaxies in the Herschel Reference Survey (HRS), a volume-limited sample of about 300 normal galaxies in the Local Universe. We find overall agreement of the EAGLE dust scaling relations with those observed in the HRS, such as the dust-to-stellar mass ratio versus stellar mass and versus NUV-r colour relations. A discrepancy in the f250/f350 versus f350/f500 submm colour-colour relation implies that part of the simulated dust is insufficiently heated, likely because of limitations in our sub-grid model for star-forming regions. We also investigate the effect of adjusting the metal-to-dust ratio and the covering factor of the photodissociation regions surrounding the star-forming cores. We are able to constrain the important dust-related parameters in our method, informing the calculation of dust attenuation for EAGLE galaxies in the UV and optical domain.

  15. Responses of parasitoids to saproxylic hosts and habitat: a multi-scale study using experimental logs.

    PubMed

    Gibb, H; Hilszczański, J; Hjältén, J; Danell, K; Ball, J P; Pettersson, R B; Alinvi, O

    2008-02-01

    Species belonging to higher trophic levels are particularly vulnerable to habitat loss and consequential host population declines, but detection of effects depends on observation scale. We investigated the effects of habitat and host availability at multiple scales on parasitoids of early successional saproxylic beetles in middle boreal Sweden, where forestry has led to habitat fragmentation and coarse woody debris (CWD) loss. Parasitoid wasps and beetle hosts were collected from nine locations, each containing three spruce-dominated stand types (clear-cut, mature managed and unmanaged stands), using emergence traps on experimental CWD. We measured local CWD volumes and determined the availability of forests of a suitable age within the landscape. We tested parasitoid responses to stand type, CWD volume, abundance of known and probable hosts and longitude. Additionally, we tested whether parasitoids responded to the area of habitat of a suitable age within radii from 0.2 to 10 km. Stand type appeared in best-fit models for all common species, suggesting that wasps respond strongly to habitat at local scales. Longitude (largely climate) featured commonly, but CWD volume was never significant. Host abundance appeared in best-fit models for three of five common species, proving significant only for Bracon obscurator, the abundance of which correlated with that of Orthotomicus laricis at both trap and site levels. Rhimphoctona spp. also correlated significantly with its known host Tetropium castaneum at the trap level. B. obscurator responded to habitat area at scales of 0.6-1 km and Cosmophorus regius responded at radii greater than 7 km, while the larger species did not respond strongly to habitat area. The role of habitat area at greater scales thus varied greatly amongst species, but our data suggest that dispersal of these common early successional species may not be strongly restricted at the current scale of fragmentation of their boreal habitats.

  16. Liquid Water Lakes on Mars Under Present-Day Conditions: Sustainability and Effects on the Subsurface

    NASA Astrophysics Data System (ADS)

    Goldspiel, Jules M.

    2015-11-01

    Decades of Mars exploration have produced ample evidence that aqueous environments once existed on the surface. Much evidence supports groundwater emergence as the source of liquid water on Mars [1-4]. However, cases have also been made for rainfall [5] and snow pack melts [6].Whatever the mechanism by which liquid water is emplaced on the surface of Mars, whether from groundwater seeps, atmospheric precipitation, or some combination of sources, this water would have collected in local topographic lows, and at least temporarily, would have created a local surface water system with dynamic thermal and hydrologic properties. Understanding the physical details of such aqueous systems is important for interpreting the past and present surface environments of Mars. It is also important for evaluating potential habitable zones on or near the surface.In conjunction with analysis of surface and core samples, valuable insight into likely past aqueous sites on Mars can be gained through modeling their formation and evolution. Toward that end, we built a 1D numerical model to follow the evolution of small bodies of liquid water on the surface of Mars. In the model, liquid water at different temperatures is supplied to the surface at different rates while the system is subjected to diurnally and seasonally varying environmental conditions. We recently simulated cases of cold (275 K) and warm (350 K) water collecting in a small depression on the floor of a mid southern latitude impact crater. When inflows create an initial pool > 3 m deep and infiltration can be neglected, we find that the interior of the pool can remain liquid over a full Mars year under the present cold and dry climate as an ice cover slowly thickens [7]. Here we present new results for the thermal and hydrologic evolution of surface water and the associated subsurface region for present-day conditions when infiltration of surface water into the subsurface is considered.[1] Pieri (1980) Science 210.[2] Carr

  17. Present-Day 3D Velocity Field of Eastern North America Based on Continuous GPS Observations

    NASA Astrophysics Data System (ADS)

    Goudarzi, Mohammad Ali; Cocard, Marc; Santerre, Rock

    2016-07-01

    The Saint Lawrence River valley in eastern Canada was studied using observations of continuously operating GPS (CGPS) stations. The area is one of the most seismically active regions in eastern North America characterized by many earthquakes, which is also subject to an ongoing glacial isostatic adjustment. We present the current three-dimensional velocity field of eastern North America obtained from more than 14 years (9 years on average) of data at 112 CGPS stations. Bernese GNSS and GITSA software were used for CGPS data processing and position time series analysis, respectively. The results show the counterclockwise rotation of the North American plate in the No-Net-Rotation model with the average of 16.8 ± 0.7 mm/year constrained to ITRF 2008. We also present an ongoing uplift model for the study region based on the present-day CGPS observations. The model shows uplift all over eastern Canada with the maximum rate of 13.7 ± 1.2 mm/year and subsidence to the south mainly over northern USA with a typical rate of -1 to -2 mm/year and the minimum value of -2.7 ± 1.4 mm/year. We compared our model with the rate of radial displacements from the ICE-5G model. Both models agree within 0.02 mm/year at the best stations; however, our model shows a systematic spatial tilt compared to ICE-5G. The misfits between two models amount to the maximum relative subsidence of -6.1 ± 1.1 mm/year to the east and maximum relative uplift of 5.9 ± 2.7 mm/year to the west. The intraplate horizontal velocities are radially outward from the centers of maximum uplift and are inward to the centers of maximum subsidence with the typical velocity of 1-1.6 ± 0.4 mm/year that is in agreement with the ICE-5G model to the first order.

  18. Importance of Past Human and Natural Disturbance in Present-Day Net Ecosystem Productivity

    NASA Astrophysics Data System (ADS)

    Felzer, B. S.; Phelps, P.

    2014-12-01

    Gridded datasets of Net Ecosystem Exchange derived from eddy covariance and remote sensing measurements provide a means of validating Net Ecosystem Productivity (NEP, opposite of NEE) from terrestrial ecosystem models. While most forested regions in the U.S. are observed to be moderate to strong carbon sinks, models not including human or natural disturbances will tend to be more carbon neutral, which is expected of mature ecosystems. We have developed the Terrestrial Ecosystems Model Hydro version (TEM-Hydro) to include both human and natural disturbances to compare against gridded NEP datasets. Human disturbances are based on the Hurtt et al. (2006) land use transition dataset and include transient agricultural (crops and pasture) conversion and abandonment and timber harvest. We include natural disturbances of storms and fires based on stochastic return intervals. Tropical storms and hurricane return intervals are based on Zheng et al. (2009) and occur only along the U.S. Atlantic and Gulf coasts. Fire return intervals are based on LANDFIRE Rapid Assessment Vegetation Models and vegetation types from the Hurtt dataset. We are running three experiments with TEM-Hydro from 1700-2011 for the conterminous U.S.: potential vegetation (POT), human disturbance only (agriculture and timber harvest, LULC), and human plus natural disturbance (agriculture, timber harvest, storms, and fire, DISTURB). The goal is to compare our NEP values to those obtained by FLUXNET-MTE (Jung et al. 2009) from 1982-2008 and ECMOD (Xiao et al., 2008) from 2000-2006 for different plant functional types (PFTs) within the conterminous U.S. Preliminary results show that, for the entire U.S., potential vegetation yields an NEP of 10.8 gCm-2yr-1 vs 128.1 gCm-2yr-1 for LULC and 89.8 gCm-2yr-1 for DISTURB from 1982-2008. The effect of regrowth following agricultural and timber harvest disturbance therefore contributes substantially to the present-day carbon sink, while stochastic storms and fires

  19. Statistical dynamical downscaling of present day and future precipitation regimes in southern Vietnam

    NASA Astrophysics Data System (ADS)

    Schubert, David; Reyers, Mark; Pinto, Joaquim; Fink, Andreas; Massmeyer, Klaus

    2016-04-01

    Southeast Asia has been identified as one of the hot-spots of climate change. While the projected changes in annual precipitation are comparatively small, there is a clear tendency towards more rainfall in the dry season and an increase in extreme precipitation events. In this study, a statistical dynamical downscaling (SDD) approach is applied to obtain higher resolution and more robust regional climate change projections for tropical Southeast Asia with focus on Vietnam. First, a recent climate (RC) simulation with the regional climate model COSMO-CLM with a spatial resolution of ~50 km driven by ERA-Interim (1979-2008) is performed for the tropical region of Southeast Asia. For the SDD, six weather types (WTs) are selected for Vietnam during the wet season (April - October) using a k-means cluster analysis of daily zonal wind component in 850 hPa and 200 hPa from the RC run. For each calculated weather type, simulated representatives are selected from the RC run and are then further dynamically downscaled to a resolution of 0.0625° (7 km). By using historical WT frequencies, the simulated representatives are recombined to a high resolution rainfall climatology for the recent climate. It is shown that the SDD is generally able to capture the present day climatology and that the employment of the higher resolved simulated representatives enhances the performance of the SDD. However, an overestimation of rainfall at higher altitudes is found. To obtain future climate projections, an ensemble of eight CMIP5 model members are selected to study precipitation changes. For these projections, WT frequencies of future scenarios under two representative Concentration Pathways (RCP4.5 and RCP8.5) are taken into account for the mid-term scenario (2046-2065) and the long-term scenario (2081-2100). The strongest precipitation changes are found for the RCP8.5 scenario. Most of the models indicate a generally increase in precipitation amount in the wet period over Southeast

  20. Estimating hypothetical present-day insured losses for past intense hurricanes in the French Antilles

    NASA Astrophysics Data System (ADS)

    Thornton, James; Desarthe, Jérémy; Naulin, Jean-Philippe; Garnier, Emmanuel; Liu, Ye; Moncoulon, David

    2015-04-01

    On the islands of the French Antilles, the period for which systematic meteorological measurements and historic event loss data are available is short relative to the recurrence intervals of very intense, damaging hurricanes. Additionally, the value of property at risk changes through time. As such, the recent past can only provide limited insight into potential losses from extreme storms in coming years. Here we present some research that seeks to overcome, as far as is possible, the limitations of record length in assessing the possible impacts of near-future hurricanes on insured properties. First, using the archives of the French overseas departments (which included administrative and weather reports, inventories of damage to houses, crops and trees, as well as some meteorological observations after 1950) we reconstructed the spatial patterns of hazard intensity associated with three historical events. They are: i) the 1928 Hurricane (Guadeloupe), ii) Hurricane Betsy (1956, Guadeloupe) and iii) Hurricane David (1979, Martinique). These events were selected because all were damaging, and the information available on each is rich. Then, using a recently developed catastrophe model for hurricanes affecting Guadeloupe, Martinique, Saint-Barthélemy and Saint-Martin, we simulated the hypothetical losses to insured properties that the reconstructed events might cause if they were to reoccur today. The model simulated damage due to wind, rainfall-induced flooding and storm surge flooding. These 'what if' scenarios provided an initial indication of the potential present-day exposure of the insurance industry to intense hurricanes. However, we acknowledge that historical events are unlikely to repeat exactly. We therefore extended the study by producing a stochastic event catalogue containing a large number of synthetic but plausible hurricane events. Instrumental data were used as a basis for event generation, but importantly the statistical methods we applied permit

  1. Multiple reciprocal adaptations and rapid genetic change upon experimental coevolution of an animal host and its microbial parasite

    PubMed Central

    Schulte, Rebecca D.; Makus, Carsten; Hasert, Barbara; Michiels, Nico K.; Schulenburg, Hinrich

    2010-01-01

    The coevolution between hosts and parasites is predicted to have complex evolutionary consequences for both antagonists, often within short time periods. To date, conclusive experimental support for the predictions is available mainly for microbial host systems, but for only a few multicellular host taxa. We here introduce a model system of experimental coevolution that consists of the multicellular nematode host Caenorhabditis elegans and the microbial parasite Bacillus thuringiensis. We demonstrate that 48 host generations of experimental coevolution under controlled laboratory conditions led to multiple changes in both parasite and host. These changes included increases in the traits of direct relevance to the interaction such as parasite virulence (i.e., host killing rate) and host resistance (i.e., the ability to survive pathogens). Importantly, our results provide evidence of reciprocal effects for several other central predictions of the coevolutionary dynamics, including (i) possible adaptation costs (i.e., reductions in traits related to the reproductive rate, measured in the absence of the antagonist), (ii) rapid genetic changes, and (iii) an overall increase in genetic diversity across time. Possible underlying mechanisms for the genetic effects were found to include increased rates of genetic exchange in the parasite and elevated mutation rates in the host. Taken together, our data provide comprehensive experimental evidence of the consequences of host–parasite coevolution, and thus emphasize the pace and complexity of reciprocal adaptations associated with these antagonistic interactions. PMID:20368449

  2. Detection of Persistent West Nile Virus RNA in Experimentally and Naturally Infected Avian Hosts

    PubMed Central

    Wheeler, Sarah S.; Langevin, Stanley A.; Brault, Aaron C.; Woods, Leslie; Carroll, Brian D.; Reisen, William K.

    2012-01-01

    To determine whether West Nile virus (WNV) persistent infection in avian hosts may potentially serve as an overwintering mechanism, House Sparrows and House Finches, experimentally and naturally infected with several strains of WNV, and two naturally infected Western Scrub-Jays were held in mosquito-proof outdoor aviaries from 2007–March 2008. Overall, 94% (n = 36) of House Sparrows, 100% (n = 14) of House Finches and 2 Western Scrub-Jays remained WNV antibody positive. When combined by species, 37% of the House Sparrows, 50% of the House Finches, and 2 Western Scrub-Jays were WNV RNA positive at necropsy, up to 36 weeks post-infection. Infectious WNV was not detected. Our study supports the hypothesis that some avian hosts support the long-term persistence of WNV RNA, but it remains unresolved whether these infections relapse to restart an avian-arthropod transmission cycle and thereby serve as an overwintering mechanism for WNV. PMID:22826479

  3. Influence of ocean tide dynamics on the climate system from the Cretaceous to present day

    NASA Astrophysics Data System (ADS)

    Weber, Tobias; Thomas, Maik

    2016-04-01

    Global numerical ocean models used for paleo-climate reconstructions generally only consider the ocean's general circulation, but neglect tidal dynamics. However, it has been demonstrated that tidally induced friction at the ocean bottom alters the mean ocean circulation and energy fluxes on timescales larger than one tidal period and up to climate timescales. Thereby the mean ocean circulation and temperature advection is altered and can thus affect climate. We simultaneously modeled the ocean's general circulation and tidal dynamics for five time-slices from the Cretaceous to present day: the Albian (ca. 110 million years ago, Ma), the Cenomanian-Turonian Boundary (ca. 93 Ma, CTB), the early Eocene (ca. 55 Ma), the early Pliocene (ca. 3.5 Ma), and a pre-industrial period (ca. 1850 AD). These simulations show that the tectonic evolution of ocean basins changes the resonance conditions in the paleo-oceans over time and thus the position of amphydromic systems and the amplitudes of partial tides. Largest amplitudes of the M2 partial tide are obtained during the early Eocene when they are in the global mean by 150% larger than in the CTB, when amplitudes are smallest. The evolution of the tidal system leads to an individual interaction between tidal dynamics and the ocean general circulation for all time-slices. In the Albian a reduction of horizontal velocities of up to 50% is simulated in the deep Indo-Pacific Throughflow (IPT) below 1000m depth. This reduction is the product of tidal residual mean currents induced by tidal waves propagating from the Pacific Ocean into the Indian Ocean that oppose the prevailing eastward thermohaline currents. In all other time-slices mainly an increase in horizontal transports is simulated. In the CTB both tidal residual mean currents (less than 0.2cm/s in most of the ocean) and the general ocean circulation (less than 0.5cm/s) are small, thus leading to a tidally induced increase by 50% in horizontal velocities in almost half of

  4. Potential autotrophic metabolisms in ultra-basic reducing springs associated with present-day continental serpentinization

    NASA Astrophysics Data System (ADS)

    Morrill, P. L.; Miles, S.; Kohl, L.; Kavanagh, H.; Ziegler, S. E.; Brazelton, W. J.; Schrenk, M. O.

    2013-12-01

    Ultra-basic reducing springs at continental sites of serpentinization act as windows into the biogeochemistry of this subsurface exothermic environment rich in H2 and CH4 gases. Biogeochemical carbon transformations in these systems are of interest because serpentinization creates conditions that are amenable to abiotic and biotic reduction of carbon. However, little is known about the metabolic capabilities of the microorganisms that live in this environment. To determine the potential for autotrophic metabolisms, bicarbonate and CO substrate addition microcosm experiments were performed using water and sediment from an ultra-basic reducing spring in the Tablelands, Newfoundland, Canada, a site of present-day continental serpentinization. CO was consistently observed to be utilized in the Live but not the Killed controlled replicates amended with 10% 13C labelled CO and non-labelled (natural C isotope abundance) CO. In the Live CO microcosms with natural C isotope abundance, the residual CO became enriched in 13C (~10 ‰) consistent with a decrease in the fraction of CO remaining. In the Killed CO controlled replicates with natural C isotope abundance the CO showed little 13C enrichment (~1.3 ‰). The data from the Live CO microcosms were well described by a Rayleigh isotopic distillation model, yielding an isotopic enrichment factor for microbial CO uptake of 15.7 ×0.5 ‰ n=2. These data suggest that there was microbial CO utilization in these experiments. The sediment and water from the 13C-labelled and non-labelled, Live and Killed microcosms were extracted for phospholipid fatty acids (PLFAs) to determine changes in community composition between treatments as well as to determine the microbial uptake of CO. The difference in community composition between the Live and Killed microcosms was not readily resolvable based on PLFA distributions. Additionally, the microbial uptake of 13CO had minimal to no affect on the δ13C of the cellular biomarkers, with the

  5. An experimental and modelling exploration of the host-sanction hypothesis in legume-rhizobia mutualism.

    PubMed

    Marco, Diana E; Carbajal, Juan P; Cannas, Sergio; Pérez-Arnedo, Rebeca; Hidalgo-Perea, Angeles; Olivares, José; Ruiz-Sainz, José E; Sanjuán, Juan

    2009-08-01

    Despite the importance of mutualism as a key ecological process, its persistence in nature is difficult to explain since the existence of exploitative, "cheating" partners that could erode the interaction is common. By analogy with the proposed policing strategy stabilizing intraspecific cooperation, host sanctions against non-N(2) fixing, cheating symbionts have been proposed as a force stabilizing mutualism in legume-Rhizobium symbiosis. Following this proposal, penalizations would include decreased nodular rhizobial viability and/or early nodule senescence in nodules occupied by cheating rhizobia. In this work, we analyse the stability of Rhizobium-legume symbiosis when non-fixing, cheating strains are present, using an experimental and modelling approach. We used split-root experiments with soybean plants inoculated with two rhizobial strains, a cooperative, normal N(2) fixing strain and an isogenic non-fixing, "perfect" cheating mutant derivative that lacks nitrogenase activity but has the same nodulation abilities inoculated to split-root plants. We found no experimental evidence of functioning plant host sanctions to cheater rhizobia based on nodular rhizobia viability and nodule senescence and maturity molecular markers. Based on these experiments, we developed a population dynamic model with and without the inclusion of plant host sanctions. We show that plant populations persist in spite of the presence of cheating rhizobia without the need of incorporating any sanction against the cheater populations in the model, under the realistic assumption that plants can at least get some amount of fixed N(2) from the effectively mutualistic rhizobia occupying some nodules. Inclusion of plant sanctions leads to the unrealistic effect of ultimate extinction of cheater strains in soil. Our simulation results are in agreement with increasing experimental evidence and theoretical work showing that mutualisms can persist in presence of cheating partners.

  6. Present-Day Seasonal Gully Activity in a South Polar Pit (Sisyphi Cavi) on Mars

    NASA Astrophysics Data System (ADS)

    Raack, Jan; Reiss, Dennis; Appéré, Thomas; Vincendon, Mathieu; Ruesch, Ottaviano; Hiesinger, Harald

    2014-05-01

    Seasonal activity of gullies under current climatic conditions on Mars was observed by [1-7]. Dundas et al. [2] reviewed the present-day activity of classical gullies (including the gully presented in this work), dune gullies, and other mass wasting processes in the southern hemisphere on Mars. Recent polar gullies in Sisyphi Cavi were also analyzed by [8], who estimated ages of about 20 ka to 20 Ma for the gullies. In this study we focus on a single gully in Sisyphi Cavi, located in the south polar region at 1.44° E and 68.54° S. The gully occurs on the gullied equator-facing slope of an isolated polar pit within an infilled impact crater. Multi-temporal high-resolution image data analyses show new deposits at the terminus of the gully channel and on the gully apron within spring (after solar longitudes of 236°) of martian years (MY) 29 and 31. In MY 29 deposition of material shortens the channel by about 40 m; in MY 31 a new deposit at the western flank of the gully apron with approximately 300-600 m3 of material is visible [3]. Our morphological investigations show that the identified new deposits were formed by dark flows through the entire gully deposited on top of the apron between LS ~218° and ~226°. Thermal data show a temperature increase between solar longitudes (LS) ~218° and ~226°. Near-infrared spectral data show relatively constant band strengths of CO2 ice and H2O ice in this time range. After the formation of the dark flows (after LS ~226°), temperatures increase rapidly from ~180 K to >~270 K at LS ~250°. At this time, spectral data indicate that all volatiles on the surface sublimated. However, an earlier beginning of sublimation when the dark flows were observed (between LS ~218° and ~226°) is likely, due to the fact that the instruments can only show the last phase of sublimation (decrease of volatile band strengths) [3]. Spectral modeling shows that from winter to mid-spring, the surface of the studied area is covered by CO2 slab

  7. Present-day formation and seasonal evolution of linear dune gullies on Mars

    NASA Astrophysics Data System (ADS)

    Pasquon, Kelly; Gargani, Julien; Massé, Marion; Conway, Susan J.

    2016-08-01

    adjacent dune) that encompass the active site. South- and SSW-facing dune slopes are those which preferentially host CO2 frost deposits, however, it is only those with angles of ∼20° just below the crest which possess linear dune gullies, suggesting a slope-limited formation process. These observations provide a wealth of temporal and morphometric data that can be used to undertake numerical modeling, to direct future image monitoring and guide laboratory experiments that can be used to better constrain the formation process of these features.

  8. Sedimentological processes and environmental variability at Lake Ohrid (Macedonia, Albania) between 640 ka and present day

    NASA Astrophysics Data System (ADS)

    Francke, A.; Wagner, B.; Just, J.; Leicher, N.; Gromig, R.; Baumgarten, H.; Vogel, H.; Lacey, J. H.; Sadori, L.; Wonik, T.; Leng, M. J.; Zanchetta, G.; Sulpizio, R.; Giaccio, B.

    2015-09-01

    Lake Ohrid (FYROM, Albania) is thought to be more than 1.2 million years old and hosts more than 200 endemic species. As a target of the International Continental Scientific Drilling Program (ICDP), a successful deep drilling campaign was carried out within the scope of the Scientific Collaboration on Past Speciation Conditions in Lake Ohrid (SCOPSCO) project in 2013. Here, we present lithological, sedimentological, and (bio-)geochemical data from the upper 247.8 m of the overall 569 m long DEEP site sediment succession from the central part of the lake. According to an age model, which is based on nine tephra layers (1st order tie points), and on tuning of biogeochemical proxy data to orbital parameters (2nd order tie points) and to the global benthic isotope stack LR04 (3rd order tie points), respectively, the analyzed sediment sequence covers the last 640 ka. The DEEP site sediment succession consists of hemipelagic sediments, which are interspersed by several tephra layers and infrequent, thin (< 5 cm) mass wasting deposits. The hemipelagic sediments can be classified into three different lithotypes. Lithotype 1 and 2 deposits comprise calcareous and slightly calcareous silty clay and are predominantly attributed to interglacial periods with high primary productivity in the lake during summer and reduced mixing during winter. The data suggest that high ion and nutrient concentrations in the lake water promoted calcite precipitation and diatom growth in the epilmnion in during MIS15, 13, and 5. Following a strong primary productivity, highest interglacial temperatures can be reported for MIS11 and 5, whereas MIS15, 13, 9, and 7 were comparable cooler. Lithotype 3 deposits consist of clastic, silty clayey material and predominantly represent glacial periods with low primary productivity during summer and longer and intensified mixing during winter. The data imply that most severe glacial conditions at Lake Ohrid persisted during MIS16, 12, 10, and 6 whereas

  9. Naturally occurring, nonregressing canine oral papillomavirus infection: host immunity, virus characterization, and experimental infection.

    PubMed

    Nicholls, P K; Klaunberg, B A; Moore, R A; Santos, E B; Parry, N R; Gough, G W; Stanley, M A

    1999-12-20

    Papillomaviruses occasionally cause severe, nonregressing or recurrent infections in their human and animal hosts. The mechanisms underlying these atypical infections are not known. Canine oral papillomavirus (COPV) typically regresses spontaneously and is an important model of mucosal human papillomavirus infections. A severe, naturally occurring, nonregressing COPV infection provided an opportunity to investigate some aspects of viral pathogenicity and host immunity. In this case, the papillomas proved refractory to surgical and medical treatments, including autogenous vaccination and vaccination with capsid (L1) virus-like particles. High levels of induced anti-L1 antibodies appeared to have no effect on the infection. The papillomas spread to oesophageal mucosa, perioral haired skin, and remote cutaneous sites. Isolation of COPV from the animal and sequencing of several regions of the viral genome showed no differences to the COPV prototype. Experimental infection of beagle dogs with this viral isolate resulted in the uncomplicated development and regression of oral warts within the usual period, indicating that the virus was not an unusual pathogenic variant. These findings support the hypothesis that the recurrent lesions seen in some human papillomavirus infections, such as recurrent laryngeal papillomatosis, are associated with specific defects in host immunity rather than variations in viral pathogenicity. PMID:10600607

  10. Present-day formation and seasonal evolution of linear dune gullies on Mars

    NASA Astrophysics Data System (ADS)

    Pasquon, Kelly; Gargani, Julien; Massé, Marion; Conway, Susan

    2016-04-01

    active site. South and SSW-facing dune slopes are those which preferentially host CO2 frost deposits, however, only those dune slopes with angles ~13° possess linear dune gullies, suggesting a slope-limited formation process. These observations provide a wealth of temporal and morphometric constraints that can be used to perform numerical modelling, to direct future image monitoring and guiding laboratory experiments which can be used to better constrain the formation process of these enigmatic features. [1] Mangold et al., 2003. J. Geophys. Res., 108 (E4), 5027. [2] Reiss et al., 2010. Geophys. Res. Lett. 37, L06203. [3] Diniega et al., 2013. Icarus 225, 526-537.

  11. Modeling soluble salt assemblages on Mars: past aqueous history and present-day habitability

    NASA Astrophysics Data System (ADS)

    Toner, J. D.; Catling, D. C.; Light, B.

    2014-12-01

    Soluble salt assemblages formed through aqueous processes are widespread on Mars. These minerals are important for understanding the past aqueous history of Mars and indicate critical habitability parameters such as pH, temperature, water activity, and salinity. Equilibrium models have been used to determine solution chemistry and salt precipitation sequences from aqueous chemical data; however, current models are limited by a lack of experimental data for low-temperature perchlorates, and some model predictions are clearly anomalous. To address the need for accurate equilibrium models, we have developed a comprehensive model for low-temperature perchlorate-rich brines using (1) previously neglected literature data, (2) experimental solubilities determined in low-temperature perchlorate solutions, and (3) solubility and heat capacity results determined using Differential Scanning Calorimetry (DSC). Our resulting model is a significant improvement over existing models, such as FREZCHEM, particularly for perchlorate mixtures. We have applied our model to evaporation and freezing of a nominal Wet Chemistry Laboratory (WCL) solution measured at the Phoenix site. For a freezing WCL solution, our model indicates that ice, KClO4, hydromagnesite (3MgCO3·Mg(OH)2·3H2O), calcite (CaCO3), meridianiite (MgSO4·11H2O), MgCl2·12H2O, NaClO4·2H2O, and Mg(ClO4)2·6H2O form at the eutectic (209 K); whereas, KClO4, hydromagnesite, kieserite (MgSO4·H2O), anhydrite (CaSO4), halite (NaCl), NaClO4·H2O, and Mg(ClO4)2·6H2O form upon complete evaporation at 298 K. In general, evaporation yields more dehydrated mineral assemblages than salts produced by freezing. Hydrated phases that form during evaporation contain 0.3 wt. % water, which compares with 1.2 wt. % during freezing. Given independent evidence for the presence of calcite and minimum water contents in Martian soils of ~1.5 wt. %, salts at the Phoenix site, and possibly elsewhere, appear more likely to have formed during

  12. Vectorial transmission of Trypanosoma cruzi: an experimental field study with susceptible and immunized hosts.

    PubMed

    Catala, S S; Gorla, D E; Basombrio, M A

    1992-07-01

    The dynamics of vectorial transmission of Trypanosoma cruzi and the level of host (guinea pigs) protection after immunization with attenuated parasites (TCC strain) was studied under natural climatic conditions in an endemic region of northern Argentina. The experimental design included two guinea pig corrals isolated by mosquito netting. One (controls) had 17 healthy and susceptible adult guinea pigs. The other had 19 guinea pigs immunized with attenuated T. cruzi TCC strain. Each corral was colonized in April 1988 with equal-sized populations of Triatoma infestans naturally infected by T. cruzi. To evaluate relevant variables in the natural transmission of Chagas' disease, corrals were sampled in both winter and late spring to assess vector populations, and to carry out parasitologic studies on both vertebrate and invertebrate hosts. In both corrals, vector density decreased in winter and reached a maximum in the hot season. The vector infection rate was very high (greater than 50%) throughout the experiment. Vector infectivity increased with temperature and vector age, but did not differ between the experimental and control corrals. The vector-host contact rate showed a close relationship with temperature, although a very high vector density decreased this rate, even with high ambient temperatures. Initial infections by T. cruzi occurred among guinea pigs only during the hot season. Vectorial transmission risk was estimated from the total number of bug bites per day, the proportion of infected bugs, and the daily incidence in the guinea pig population. During the hot season, this risk was 6.84 x 10(-4) in the control group and 1.82 x 10(-4) in the immunized group. PMID:1636879

  13. Modelling of hybrid scenario: from present-day experiments towards ITER

    NASA Astrophysics Data System (ADS)

    Litaudon, X.; Voitsekhovitch, I.; Artaud, J. F.; Belo, P.; Bizarro, João P. S.; Casper, T.; Citrin, J.; Fable, E.; Ferreira, J.; Garcia, J.; Garzotti, L.; Giruzzi, G.; Hobirk, J.; Hogeweij, G. M. D.; Imbeaux, F.; Joffrin, E.; Koechl, F.; Liu, F.; Lönnroth, J.; Moreau, D.; Parail, V.; Schneider, M.; Snyder, P. B.; the ASDEX-Upgrade Team; Contributors, JET-EFDA; the EU-ITM ITER Scenario Modelling Group

    2013-07-01

    The ‘hybrid’ scenario is an attractive operating scenario for ITER since it combines long plasma duration with the reliability of the reference H-mode regime. We review the recent European modelling effort carried out within the Integrated Scenario Modelling group which aims at (i) understanding the underlying physics of the hybrid regime in ASDEX-Upgrade and JET and (ii) extrapolating them towards ITER. JET and ASDEX-Upgrade hybrid scenarios performed under different experimental conditions have been simulated in an interpretative and predictive way in order to address the current profile dynamics and its link with core confinement, the relative importance of magnetic shear, s, and E × B flow shear on the core turbulence, pedestal stability and H-L transition. The correlation of the improved confinement with an increased s/q at outer radii observed in JET and ASDEX-Upgrade discharges is consistent with the predictions based on the GLF23 model applied in the simulations of the ion and electron kinetic profiles. Projections to ITER hybrid scenarios have been carried out focusing on optimization of the heating/current drive schemes to reach and ultimately control the desired plasma equilibrium using ITER actuators. Firstly, access condition to the hybrid-like q-profiles during the current ramp-up phase has been investigated. Secondly, from the interpreted role of the s/q ratio, ITER hybrid scenario flat-top performance has been optimized through tailoring the q-profile shape and pedestal conditions. EPED predictions of pedestal pressure and width have been used as constraints in the interpretative modelling while the core heat transport is predicted by GLF23. Finally, model-based approach for real-time control of advanced tokamak scenarios has been applied to ITER hybrid regime for simultaneous magnetic and kinetic profile control.

  14. Body wave travel times and amplitudes for present-day seismic model of Mars

    NASA Astrophysics Data System (ADS)

    Raevskiy, Sergey; Gudkova, Tamara

    At the moment Martian interior structure models are constrained by the satellite observational data (the mass, the moment of inertia factor, the Love number k _{2}) (Konopliv et al., 2011) and high pressure experimental data (Bertka and Fei, 1997). Seismological observations could provide unparalleled capability for studying Martian interiors. Future missions include seismic experiments on Mars (Lognonné et al., 2012). The main instrument for these seismic experiments is a broadband seismometer (Robert et al., 2012). When seismic measurements are not yet available, physically consistent interior models, characterized by properties of relevant minerals, make possible to study of the seismic response of the planet. \\To estimate travel times for direct P, S, core reflected PcP, ScS and core refracted PKP body waves as a function of epicentral distance and hypocentral depth, as well as their amplitudes at the surface for a given marsquake, software product was developed in MatLab, as it encompasses many plotting routines that plot resulting travel times and ray paths. The computational results have been compared with the program TTBox (Knapmeyer, 2004). The code computes seismic ray paths and travel times for a one-dimentional spherical interior model (density and seismic velocities are functions of a radius only). Calculations of travel times tables for direct P, S, core reflected PcP, ScS and core refracted PKP waves and their amplitudes are carried out for a trial seismic model of Mars M14_3 from (Zharkov et al., 2009): the core radius is 1800 km, the thickness of the crust is 50 km. Direct and core reflected P and S waves are recorded to a maximum epicentral distance equal to about 100(°) , and PKP arrivals can be detected for epicental distances larger than 150(°) . The shadow zone is getting wider in comparison with previous results (Knapmeyer, 2010), as the liquid core radius of the seismic model under consideration is larger. Based on the estimates of

  15. Increased survival of experimentally evolved antimicrobial peptide-resistant Staphylococcus aureus in an animal host.

    PubMed

    Dobson, Adam J; Purves, Joanne; Rolff, Jens

    2014-09-01

    Antimicrobial peptides (AMPs) have been proposed as new class of antimicrobial drugs, following the increasing prevalence of bacteria resistant to antibiotics. Synthetic AMPs are functional analogues of highly evolutionarily conserved immune effectors in animals and plants, produced in response to microbial infection. Therefore, the proposed therapeutic use of AMPs bears the risk of 'arming the enemy': bacteria that evolve resistance to AMPs may be cross-resistant to immune effectors (AMPs) in their hosts. We used a panel of populations of Staphylococcus aureus that were experimentally selected for resistance to a suite of individual AMPs and antibiotics to investigate the 'arming the enemy' hypothesis. We tested whether the selected strains showed higher survival in an insect model (Tenebrio molitor) and cross-resistance against other antimicrobials in vitro. A population selected for resistance to the antimicrobial peptide iseganan showed increased in vivo survival, but was not more virulent. We suggest that increased survival of AMP-resistant bacteria almost certainly poses problems to immune-compromised hosts.

  16. Increased survival of experimentally evolved antimicrobial peptide-resistant Staphylococcus aureus in an animal host.

    PubMed

    Dobson, Adam J; Purves, Joanne; Rolff, Jens

    2014-09-01

    Antimicrobial peptides (AMPs) have been proposed as new class of antimicrobial drugs, following the increasing prevalence of bacteria resistant to antibiotics. Synthetic AMPs are functional analogues of highly evolutionarily conserved immune effectors in animals and plants, produced in response to microbial infection. Therefore, the proposed therapeutic use of AMPs bears the risk of 'arming the enemy': bacteria that evolve resistance to AMPs may be cross-resistant to immune effectors (AMPs) in their hosts. We used a panel of populations of Staphylococcus aureus that were experimentally selected for resistance to a suite of individual AMPs and antibiotics to investigate the 'arming the enemy' hypothesis. We tested whether the selected strains showed higher survival in an insect model (Tenebrio molitor) and cross-resistance against other antimicrobials in vitro. A population selected for resistance to the antimicrobial peptide iseganan showed increased in vivo survival, but was not more virulent. We suggest that increased survival of AMP-resistant bacteria almost certainly poses problems to immune-compromised hosts. PMID:25469169

  17. The effect of different zooxanthellae on the growth of experimentally reinfected hosts.

    PubMed

    Kinzie, R A; Chee, G S

    1979-06-01

    1. A method is given enabling the differential effects of different strains of zooxanthellae on host growth to be assessed. This technique uses the increase in the number of tentacles as the measure of growth. 2. Aposymbiotic polyps of the anemone Aiptasia pulchella reinfected with strains of Symbiodinium microadriaticum isolated from the anemone Aiptasia pulchella and the scyphozoan Cassiopea xamachana grow as well as normal Aiptasia polyps. 3. Aposymbiotic Aiptasia polyps reinfected with zooxanthellae from the gastropod Melibe pilosa and the clam Tridacna maxima grew no better than polyps lacking zooxanthellae. 4. These results lead to the conclusion that strains of zooxanthellae differ in their ability to enhance growth of Aiptasia polyps under the experimental conditions and that these differences may have important ecological consequences. PMID:36925

  18. Host specificity of North American Rhabdias spp. (Nematoda: Rhabdiasidae): combining field data and experimental infections with a molecular phylogeny.

    PubMed

    Langford, Gabriel J; Janovy, John

    2013-04-01

    Lungworms of the cosmopolitan genus Rhabdias are among the most common parasites of amphibians and squamate reptiles. The present study used experimental infections, field studies, and a molecular phylogeny to determine the host specificity of 6 Rhabdias spp. that infect snakes and anurans from North America. The molecular phylogeny suggests Rhabdias ranae from Nebraska and Mississippi may represent separate, cryptic species. In addition, the phylogeny strongly supports separate clades for anuran and snake lungworms. Field studies and experimental infections indicate that snake lungworms are generalist snake parasites; however, laboratory experiments also suggest that lizards can be infected under some environmental conditions. Lungworms from anurans were found not to infect salamanders or reptiles, in nature or in the laboratory; anuran lungworm species ranged from strict host specificity, e.g., R. ranae from Nebraska, to relative generalist, e.g., Rhabdias joaquinensis from Nebraska. Overall, host specificity for species of Rhabdias does not provide support for the evolution of progressive specialization over time. For most species of lungworms, host specificity in nature appears to be limited by both ecological and physiological factors, which vary between species and their hosts. Furthermore, some lungworms, e.g., Rhabdias bakeri from Missouri, appear to be tracking host resources instead of host phylogenies, an example of ecological fitting. PMID:22988815

  19. Experimental evolution of parasitoid infectivity on symbiont-protected hosts leads to the emergence of genotype specificity.

    PubMed

    Rouchet, Romain; Vorburger, Christoph

    2014-06-01

    Host-parasitoid interactions may lead to strong reciprocal selection for traits involved in host defense and parasitoid counterdefense. In aphids, individuals harboring the facultative bacterial endosymbiont, Hamiltonella defensa, exhibit enhanced resistance to parasitoid wasps. We used an experimental evolution approach to investigate the ability of the parasitoid wasp, Lysiphlebus fabarum, to adapt to the presence of H. defensa in its aphid host Aphis fabae. Sexual populations of the parasitoid were exposed for 11 generations to a single clone of A. fabae, either free of H. defensa or harboring artificial infections with three different isolates of H. defensa. Parasitoids adapted rapidly to the presence of H. defensa in their hosts, but this adaptation was in part specific to the symbiont isolate they were evolving against and did not result in an improved infectivity on all symbiont-protected hosts. Comparisons of life-history traits among the evolved lines of parasitoids did not reveal any evidence for costs of adaptation to H. defensa in terms of correlated responses that could constrain such adaptation. These results show that parasitoids readily evolve counter-adaptations to heritable defensive symbionts of their hosts, but that different symbiont strains impose different evolutionary challenges. The symbionts thus mediate the host-parasite interaction by inducing line-by-line genetic specificity.

  20. Simulating the Greenland ice sheet under present-day and palaeo constraints including a new discharge parameterization

    NASA Astrophysics Data System (ADS)

    Calov, R.; Robinson, A.; Perrette, M.; Ganopolski, A.

    2015-02-01

    In this paper, we propose a new sub-grid scale parameterization for the ice discharge into the ocean through outlet glaciers and inspect the role of different observational and palaeo constraints for the choice of an optimal set of model parameters. This parameterization was introduced into the polythermal ice-sheet model SICOPOLIS, which is coupled to the regional climate model of intermediate complexity REMBO. Using the coupled model, we performed large ensemble simulations over the last two glacial cycles by varying two major parameters: a melt parameter in the surface melt scheme of REMBO and a discharge scaling parameter in our parameterization of ice discharge. Our empirical constraints are the present-day Greenland ice sheet surface elevation, the surface mass balance partition (ratio between total ice discharge and total precipitation) and the Eemian interglacial elevation drop relative to present day in the vicinity of the NEEM ice core. We show that the ice discharge parameterization enables us to simulate both the correct ice-sheet shape and mass balance partition at the same time without explicitly resolving the Greenland outlet glaciers. For model verification, we compare the simulated total and sectoral ice discharge with other estimates. For the model versions that are consistent with the range of observational and palaeo constraints, our simulated Greenland ice sheet contribution to Eemian sea-level rise relative to present-day amounts to 1.4 m on average (in the range of 0.6 and 2.5 m).

  1. Experimental evidence of negative interspecific interactions among imago fleas: flea and host identities matter.

    PubMed

    Khokhlova, Irina S; Dlugosz, Elizabeth M; Krasnov, Boris R

    2016-03-01

    We investigated interspecific interactions between two flea species (Parapulex chephrenis and Xenopsylla ramesis) via evaluation of their feeding success (the size of a blood meal and time to death after a single blood meal) when they exploited rodent hosts [Acomys cahirinus (a characteristic host of the former) or Meriones crassus (a characteristic host of the latter)] in single-species or mixed-species groups. We predicted that the negative interactions between the two fleas will result in smaller blood meals and shorter survival time in mixed- versus single-species infestations. We also predicted that the negative effect of mixed-species infestation on feeding performance would be less pronounced when fleas exploited their characteristic host rather than a non-characteristic host. When exploiting a characteristic host, P. chephrenis took larger blood meals in single- than in mixed-species groups, whereas the blood meal size in X. ramesis did not differ between treatments. When exploiting a non-characteristic host, no effect of group composition was found in either flea species. Survival time after a single blood meal was not affected by co-infestation or host species in either flea. Our results suggest context-dependence of the negative effect of co-infestation on feeding performance in fleas with the manifestation of this effect varying in dependence of flea and host species identities.

  2. Assessment of present day geomorphological dynamics to decipher landscape evolution around the Paleolithic sites of Melka Kunture, Ethiopia

    NASA Astrophysics Data System (ADS)

    Maerker, Michael; Schillaci, Calogero; Melis, Rita; Mussi, Margherita

    2014-05-01

    The area of Melka Kunture (central Ethiopia) is one of the most important clusters of Paleolithic sites in Eastern Africa. The archaeological record spans from c. 1.7 Ma onwards, with a number of stratified occurrences of Oldowan, Acheulean, Middle Stone Age and Late Stone Age industries, together with faunal remains and human fossils. However, the archaeological sites are endangered by flooding and soil erosion. The main excavation area lies close to the convergence of the Awash river with the Atabella river, one of the main tributaries of the upper Awash catchment. In the semi-arid Ethiopian highlands, gully networks develop especially in the vicinity of the active and inactive river meanders. Various erosion processes are linked to specific driving factors such as the rainfall regime, the land use/cover changes and vertic soils with a specific hydrological behaviour. It was documented in the field and by previous research that the origin of most of the man made erosion channels is due to animal pathways and car tracks. However, paleolandscape features increase the general erosion risk. Former wetland areas and deposition zones are particularly affected by soil erosion processes. Hence, the spatial distribution and characteristics of present day geomorphic processes also reveal information on the paleolandscape. In order to assess landscape evolution and present day geomorphologic dynamics, we mapped the geomorphology describing in detail the present-day slope processes at a 10.000 scale. We performed a detailed terrain analysis based on high resolution DEMs such as SRTM-X with 25m resolution and ALOS/PRISM with 10m resolution to characterize the main erosion processes and surface runoff dynamics. The latter ones are simulated using a Soil Conservation Service Curve Number method. Landuse was delineated for a larger area using ASTER 25m multispectral data. Finally, using calibrated topographic indices and a simple hydrological model we were able to detect and

  3. The present-day strain partitioning of the Western Alps and its relationships with the crustal scale geometry

    NASA Astrophysics Data System (ADS)

    Schwartz, S.; Lardeaux, J. M.; Tricart, P.; Paul, A.; Béthoux, N.; Masson, F.

    2003-04-01

    In the orogenic belt, the combination of the geological data (tectonic, petrologic and kinematic analysis) with the geophysical imagery of the crust (regional seismicity, local earthquake tomography and gravimetry) allows to obtain crustal scale models coherent with the present-day deformation. In this way, the recent pluridisciplinary studies performed in the framework of " Geo-France 3D Alpes " show an important syn-convergence strain-partitioning at the scale of the western alpine belt. In the internal domain, this strain-partitioning, corresponds to extensional tectonics combinated with important strike-slip components while the external domain and the Po plain, located at the periphery, record an important shortening accomodated in surface by thrust and strike-slip faults. In order to understand the alpine present-day strain pattern, we propose and discuss a new crustal-scale cross-section performed along the Pelvoux-Dora Maira transect. The proeminent features of this crustal-scale geometry are : -the existence of a slice of cold and rigid mantle of Apulian origin beneath the Dora Maira massif. This rigid mantle is truncated in two distinct bodies by a system of deeply-rooted vertical faults related to the Insubric line. -these two main bodies of mantle acted as indenters driving the decoupling of the European crust and at least a part of the exhumation of the high-pressure metamorphic units. -the deep architecture is characterized by the stacking of crustal slices detached from the European lithosphere. Some of these slices represent tectonic extrusions within the overlying Piemont Schistes lustrés. -the Monviso eclogitized ophiolites are plunging up to 20 km depth below the Piemont schistes lustrés. In this geometrical model, the decoupling of the mantle identor drives the crustal-scale strain-partitioning of the western Alps. Indeed, the lower part of the rigid mantle transfers the compression onto the european foreland and is responsible for the present-day

  4. Experimental infection dynamics: using immunosuppression and in vivo parasite tracking to understand host resistance in an amphibian-trematode system.

    PubMed

    LaFonte, Bryan E; Johnson, Pieter T J

    2013-10-01

    Although naturally occurring hosts often exhibit pronounced differences in infection and pathology, the relative importance of factors associated with host life history and immunity in explaining such patterns often remains speculative. Research in eco-immunology highlights the trade-offs between host physiology and immunity, for which natural variations in disease susceptibility offer a valuable platform to test predictions within this framework. Here, we combined use of a novel, in vivo assay for tracking parasite fate and an experimental manipulation of host immune function (via chronic corticosterone exposure) to assess the role of host immunity in regulating susceptibility of amphibian hosts to three larval trematodes: Ribeiroia ondatrae, Echinostoma trivolvis and Alaria sp. 2. Results from the in vivo parasite-tracking assay revealed marked differences in initial parasite penetration and subsequent host clearance. Relative to infections in a highly susceptible species (Pseudacris regilla), the virulent trematode R. ondatrae was -25% less successful at penetrating larvae of three hylid frog species and was cleared > 45(×) faster, such that all parasites were rapidly cleared from hylid hosts over 72 h following a Weibull distribution. Immune suppression of Hyla versicolor sharply reduced this resistance and increased infection of all three trematodes by 67 to 190%, with particularly strong increases for R. ondatrae. Diminished resistance correlated with a 62% decrease in circulating eosinophils. Correspondingly, 10 days after corticosterone exposures ended, infections declined dramatically while eosinophil levels returned to normal. In light of ongoing declines and deformities in amphibian populations, these findings have application potential for mitigating disease-driven effects.

  5. Iceland hotspot track in southeast Greenland causes huge present-day vertical viscoelastic motion of the bedrock

    NASA Astrophysics Data System (ADS)

    Khan, Shfaqat Abbas; Sasgen, Ingo; Bevis, Michael; van Dam, Tonie; Wahr, John; Bamber, Jonathan; Wouters, Bert; Helm, Veit; Willis, Michael; Csatho, Beata; Knudsen, Per; Kuipers Munneke, Peter; Kjær, Kurt

    2016-04-01

    The process of Glacial Isostatic Adjustment (GIA) represents the ongoing response of the solid Earth to past ice mass loss that occurred following the Last Glacial Maximum (LGM, ~21 ka B.P.). The magnitude of the GIA uplift depends on the temporal history of the ice load and is highly sensitive to variations in upper mantle viscosity. Greenland GIA is thought to be well contained and due to relative high viscosity, influence of more recent changes e.g. since the Little Ice Age have minor present-day effect (<2 mm/yr). Here we use data from the Greenland Global Positioning System (GPS) network to measure GIA. We identify an unexpected GIA anomaly of ~12 mm/yr in southeast Greenland, which we interpret as linked to a zone of warmer upper mantle caused by the Iceland hotspot track that would reduce the viscosity and produce greater viscoelastic uplift due to recent ice mass changes. We reconsider the evolution of the Greenland ice sheet since LGM and estimate a total ice mass loss equivalent to sea level rise of 4.9 m since LGM. Our observations suggest southeast and northwest Greenland, subject to present-day major ice loss, also contributed by significantly more mass loss on millennia scale than previously estimated.

  6. Present-day and future Antarctic ice sheet climate and surface mass balance in the Community Earth System Model

    NASA Astrophysics Data System (ADS)

    Lenaerts, Jan T. M.; Vizcaino, Miren; Fyke, Jeremy; van Kampenhout, Leo; van den Broeke, Michiel R.

    2016-02-01

    We present climate and surface mass balance (SMB) of the Antarctic ice sheet (AIS) as simulated by the global, coupled ocean-atmosphere-land Community Earth System Model (CESM) with a horizontal resolution of ˜1° in the past, present and future (1850-2100). CESM correctly simulates present-day Antarctic sea ice extent, large-scale atmospheric circulation and near-surface climate, but fails to simulate the recent expansion of Antarctic sea ice. The present-day Antarctic ice sheet SMB equals 2280 ± 131 {Gt year^{-1}} , which concurs with existing independent estimates of AIS SMB. When forced by two CMIP5 climate change scenarios (high mitigation scenario RCP2.6 and high-emission scenario RCP8.5), CESM projects an increase of Antarctic ice sheet SMB of about 70 {Gt year^{-1}} per degree warming. This increase is driven by enhanced snowfall, which is partially counteracted by more surface melt and runoff along the ice sheet's edges. This intensifying hydrological cycle is predominantly driven by atmospheric warming, which increases (1) the moisture-carrying capacity of the atmosphere, (2) oceanic source region evaporation, and (3) summer AIS cloud liquid water content.

  7. Present-day and future Antarctic ice sheet climate and surface mass balance in the Community Earth System Model

    NASA Astrophysics Data System (ADS)

    Lenaerts, Jan T. M.; Vizcaino, Miren; Fyke, Jeremy; van Kampenhout, Leo; van den Broeke, Michiel R.

    2016-09-01

    We present climate and surface mass balance (SMB) of the Antarctic ice sheet (AIS) as simulated by the global, coupled ocean-atmosphere-land Community Earth System Model (CESM) with a horizontal resolution of {˜ }1° in the past, present and future (1850-2100). CESM correctly simulates present-day Antarctic sea ice extent, large-scale atmospheric circulation and near-surface climate, but fails to simulate the recent expansion of Antarctic sea ice. The present-day Antarctic ice sheet SMB equals 2280 ± 131 {Gt year^{-1}}, which concurs with existing independent estimates of AIS SMB. When forced by two CMIP5 climate change scenarios (high mitigation scenario RCP2.6 and high-emission scenario RCP8.5), CESM projects an increase of Antarctic ice sheet SMB of about 70 {Gt year^{-1}} per degree warming. This increase is driven by enhanced snowfall, which is partially counteracted by more surface melt and runoff along the ice sheet's edges. This intensifying hydrological cycle is predominantly driven by atmospheric warming, which increases (1) the moisture-carrying capacity of the atmosphere, (2) oceanic source region evaporation, and (3) summer AIS cloud liquid water content.

  8. Isolation and prominent aboriginal maternal legacy in the present-day population of La Gomera (Canary Islands)

    PubMed Central

    Fregel, Rosa; Cabrera, Vicente M; Larruga, José M; Hernández, Juan C; Gámez, Alejandro; Pestano, Jose J; Arnay, Matilde; González, Ana M

    2015-01-01

    The present-day population structure of La Gomera is outstanding in its high aboriginal heritage, the greatest in the Canary Islands. This was earlier confirmed by both mitochondrial DNA and autosomal analyses, although genetic drift due to the fifteenth century European colonization could not be excluded as the main factor responsible. The present mtDNA study of aboriginal remains and extant samples from the six municipal districts of the island indeed demonstrates that the pre-Hispanic colonization of La Gomera by North African people involved a strong founder event, shown by the high frequency of the indigenous Canarian U6b1a lineage in the aboriginal samples (65%). This value is even greater than that observed in the extant population (44%), which in turn is the highest of all the seven Canary Islands. In contrast to previous results obtained for the aboriginal populations of Tenerife and La Palma, haplogroups related to secondary waves of migration were not detected in La Gomera aborigines, indicating that isolation also had an important role in shaping the current population. The rugged relief of La Gomera divided into several distinct valleys probably promoted subsequent aboriginal intra-insular differentiation that has continued after the European colonization, as seen in the present-day population structure observed on the island. PMID:25407001

  9. An experimental conflict of interest between parasites reveals the mechanism of host manipulation

    PubMed Central

    Milinski, Manfred

    2016-01-01

    Parasites can increase their host’s predation susceptibility. It is a long-standing puzzle, whether this is caused by host manipulation, an evolved strategy of the parasite, or by side effects due to, for example, the parasite consuming energy from its host thereby changing the host’s trade-off between avoiding predation and foraging toward foraging. Here, we use sequential infection of three-spined sticklebacks with the cestode Schistocephalus solidus so that parasites have a conflict of interest over the direction of host manipulation. With true manipulation, the not yet infective parasite should reduce rather than enhance risk taking because predation would be fatal for its fitness; if host behavior is changed by a side effect, the 2 parasites would add their increase of predation risk because both drain energy. Our results support the latter hypothesis. In an additional experiment, we tested both infected and uninfected fish either starved or satiated. True host manipulation should act independently of the fish’s hunger status and continue when energy drain is balanced through satiation. Starvation and satiation affect the risk averseness of infected sticklebacks similarly to that of uninfected starved and satiated ones. Increased energy drain rather than active host manipulation dominates behavioral changes of S. solidus-infected sticklebacks. PMID:27004014

  10. [Effects of host plants on the life table parameters of experimental populations of Aphis gossypii].

    PubMed

    Li, Yan-Yan; Zhou, Xiao-Rong; Pang, Bao-Ping; Chang, Jing

    2013-05-01

    A comparative study was conducted on the life table parameters of Aphis gossypii reared on five host plant species at (25 +/- 1) degrees C in laboratory. There existed significant differences in the durations of various developmental stages, adult longevity, mean offspring number per day, net reproductive rate, intrinsic rate of increase, finite rate of increase, mean generation time, and population doubling time for the A. gossypii populations reared on the host plants. For the aphids on Lagenaria siceraria var. turbinate, they needed the longest time (5.84 days) to complete one generation, but for those on the other four plants, no significant differences were observed, with the time needed ranged from 5.24 to 5.45 days. The adult longevity was the longest (20.04 days) on Cucumis sativus, but had no significant differences on the other four host plants, being from 14.76 to 16.03 days. The populations' survival curves on all test host plants were of Deevey I, i. e., the death mainly occurred during late period. The survival rate on C. sativus was higher than those on the other four host plants. Based on the intrinsic rates of increase of A. gossypii, its host suitability was in the order of Cucumis melo var. saccharinus > Lagenaria siceraria var. turbinate > Cucurbita moschata var. melonaeformis > Cucumis sativus > Cucurbita pepo var. medullosa.

  11. Unraveling the geochemistry of melts in exhumed mantle domains in present-day and fossil magma-poor rifted margins

    NASA Astrophysics Data System (ADS)

    Amann, Méderic; Ulrich, Marc; Autin, Julia; Manatschal, Gianreto; Epin, Marie-Eva; Müntener, Othmar; Boiron, Marie-Christine; Sauter, Daniel

    2016-04-01

    The role of magmatic processes occurring during the continental break-up and the onset of steady-state seafloor spreading are still a matter of debate. Beside the tectonic processes like stretching, thinning and exhumation, magmatic processes also play a key role in the evolution and breakup of magma-poor rifted margins. To unravel the impact of such processes, Ocean-Continent-Transitions (OCTs) are of particular interest. OCTs are complex areas where hyper-extended continental crust, exhumed mantle and proto-oceanic crust occur. All these domains have been identified and sampled in both present-day (Iberia/Newfoundland margins) and fossil margins (Platta/Err nappes). In this study, we present preliminary results that enable to characterize the nature of the mantle rocks and the melts found in the OCTs of these paleo- and present-day margins with the aim to investigate how the mantle evolves from initial exhumation to final lithospheric breaks. In OCTs two types of mantle rocks can be observed: (i) a « sub-continental type » free of syn-exhumation melt imprint preserving the early geochemical evolution, and (ii) a « refertilized type » characterized by melt infiltration and mantle-melt interaction. Melts from these domains have different major, trace element and isotopic compositions and can therefore be used to constrain how melt interacts with the mantle and to understand the role of magmatic processes in the break-up. We therefore summarized whole-rock, in-situ and isotopic analysis available in the literature from the Iberia/Newfoundland present-day margin system and completed the existing database with new additional data from the Iberia margin. These new data have been obtained using in-situ technics mainly on clinopyroxenites, serpentinized peridotites and gabbros of ODP drill cores. Around 200 new data have been acquired using the LA-ICPMS technic. Preliminary results show that clinopyroxenes in serpentinized peridodite breccia from ODP site 637A and

  12. Oxidation of dissolved iron under warmer, wetter conditions on Mars: Transitions to present-day arid environments

    NASA Astrophysics Data System (ADS)

    Burns, R. G.

    The copious deposits of ferric-iron assemblages littering the surface of bright regions of Mars indicate that efficient oxidative weathering reactions have taken place during the evolution of the planet. Because the kinetics of atmosphere-surface (gas-solid) reactions are considerably slower than chemical weathering reactions involving an aqueous medium, most of the oxidation products now present in the martian regolith probably formed when groundwater flowed near the surface. This paper examines how chemical weathering reactions were effected by climatic variations when warm, wet environments became arid on Mars. Analogies are drawn with hydrogeochemical and weathering environments on the Australian continent where present-day oxidation of iron is occurring in acidic ground water under arid conditions.

  13. Oxidation of dissolved iron under warmer, wetter conditions on Mars: Transitions to present-day arid environments

    NASA Technical Reports Server (NTRS)

    Burns, R. G.

    1993-01-01

    The copious deposits of ferric-iron assemblages littering the surface of bright regions of Mars indicate that efficient oxidative weathering reactions have taken place during the evolution of the planet. Because the kinetics of atmosphere-surface (gas-solid) reactions are considerably slower than chemical weathering reactions involving an aqueous medium, most of the oxidation products now present in the martian regolith probably formed when groundwater flowed near the surface. This paper examines how chemical weathering reactions were effected by climatic variations when warm, wet environments became arid on Mars. Analogies are drawn with hydrogeochemical and weathering environments on the Australian continent where present-day oxidation of iron is occurring in acidic ground water under arid conditions.

  14. Three-dimensional velocity field of present-day crustal motion of the Tibetan Plateau derived from GPS measurements

    NASA Astrophysics Data System (ADS)

    Liang, Shiming; Gan, Weijun; Shen, Chuanzheng; Xiao, Genru; Liu, Jing; Chen, Weitao; Ding, Xiaoguang; Zhou, Deming

    2013-10-01

    the measurements of 750 GPS stations around the Tibetan Plateau for over 10 years since 1999, we derived a high-resolution 3-D velocity field for the present-day crustal movement of the plateau. The horizontal velocity field relative to stable Eurasia displays in details the crustal movement and tectonic deformation features of the India-Eurasia continental collision zone with thrust compression, lateral extrusion, and clockwise rotation. The vertical velocity field reveals that the Tibetan Plateau is continuing to rise as a whole relative to its stable north neighbor. However, in some subregions, uplift is insignificant or even negative. The main features of the vertical crustal deformation of the plateau are the following: (a) The Himalayan range is still rising at a rate of ~2 mm/yr. The uplift rate is ~6 mm/yr with respect to the south foot of the Himalayan range. (b) The middle eastern plateau has a typical uplift rate between 1 and 2 mm/yr, and some high mountain ranges in this area, like the Longmen Shan and Gongga Shan, have surprising uplift rates as large as 2-3mm/yr. (c) In the middle southern plateau, there is a basin and endorheic subregion with a series of NS striking normal faults, showing obvious sinking with the rates between 0 and -3 mm/yr. (d) The present-day rising and sinking subregions generally correspond well to the Cenozoic orogenic belts and basins, respectively. (e) At the southeastern corner of the plateau. There is an apparent trend that the uplift rate is gradually decreasing from between 0.8 and 2.3 mm/yr in the inner plateau to between -0.5 and -1.6 mm/yr outside the plateau, with the decrease of terrain height.

  15. A comparison of quality of present-day heat flow obtained from BHTs, Horner Plots of Malay Basin

    SciTech Connect

    Waples, D.W.; Mahadir, R. )

    1994-07-01

    Reconciling temperature data obtained from measurement of single BHT, multiple BHT at a single depth, RFTs, and DSTs, is very difficult. Quality of data varied widely, however DST data were assumed to be most reliable. Data from 87 wells was used in this study, but only 47 wells have DST data. BASINMOD program was used to calculate the present-day heat flow, using measured thermal conductivity and calibrated against the DST data. The heat flows obtained from the DST data were assumed to be correct and representative throughout the basin. Then, heat flows using (1) uncorrected RFT data, (2) multiple BHT data corrected by the Horner plot method, and (3) single BHT values corrected upward by a standard 10% were calculated. All of these three heat-flow populations had identically standard deviations to that for the DST data, but with significantly lower mean values. Correction factors were calculated to give each of the three erroneous populations the same mean value as the DST population. Heat flows calculated from RFT data had to be corrected upward by a factor of 1.12 to be equivalent to DST data; Horner plot data corrected by a factor of 1.18, and single BHT data by a factor of 1.2. These results suggest that present-day subsurface temperatures using RFT, Horner plot, and BHT data are considerably lower than they should be. The authors suspect qualitatively similar results would be found in other areas. Hence, they recommend significant corrections be routinely made until local calibration factors are established.

  16. Present-day oxidative subsidence of organic soils and mitigation in the Sacramento-San Joaquin Delta, California, USA

    NASA Astrophysics Data System (ADS)

    Deverel, Steven J.; Ingrum, Timothy; Leighton, David

    2016-05-01

    Subsidence of organic soils in the Sacramento-San Joaquin Delta threatens sustainability of the California (USA) water supply system and agriculture. Land-surface elevation data were collected to assess present-day subsidence rates and evaluate rice as a land use for subsidence mitigation. To depict Delta-wide present-day rates of subsidence, the previously developed SUBCALC model was refined and calibrated using recent data for CO2 emissions and land-surface elevation changes measured at extensometers. Land-surface elevation change data were evaluated relative to indirect estimates of subsidence and accretion using carbon and nitrogen flux data for rice cultivation. Extensometer and leveling data demonstrate seasonal variations in land-surface elevations associated with groundwater-level fluctuations and inelastic subsidence rates of 0.5-0.8 cm yr-1. Calibration of the SUBCALC model indicated accuracy of ±0.10 cm yr-1 where depth to groundwater, soil organic matter content and temperature are known. Regional estimates of subsidence range from <0.3 to >1.8 cm yr-1. The primary uncertainty is the distribution of soil organic matter content which results in spatial averaging in the mapping of subsidence rates. Analysis of leveling and extensometer data in rice fields resulted in an estimated accretion rate of 0.02-0.8 cm yr-1. These values generally agreed with indirect estimates based on carbon fluxes and nitrogen mineralization, thus preliminarily demonstrating that rice will stop or greatly reduce subsidence. Areas below elevations of -2 m are candidate areas for implementation of mitigation measures such as rice because there is active subsidence occurring at rates greater than 0.4 cm yr-1.

  17. 3D velocity field of present-day crustal motion of the Tibetan Plateau derived from GPS measurements

    NASA Astrophysics Data System (ADS)

    Gan, W.

    2013-12-01

    Using the measurements of 564 GPS stations around the Tibetan plateau for over 10 years, we derived a high-resolution 3D velocity field for the present-day crustal motion of the plateau with improved precision. The horizontal velocity field of the plateau relative to stable Eurasia displays in details the crustal movement and tectonic deformation features of India-Eurasia continental collision zone with thrust compression, lateral extrusion and clockwise rotation. The vertical velocities reveal that the plateau is still rising as a whole relative to its stable north neighbor. However, in some subregions uplift is insignificant or even negative. The main features of the vertical crustal deformation are: a) The Himalayan range is rising at a rate of ~3mm/yr, the most significant in the whole plateau. The uplift rate of the Himalayan range is ~6mm/a relative to its south foot; b) The mid-eastern plateau has an typical uplift rate between 1~2 mm/a, and some high mountain ranges in this area have surprising uplift rates as large as 2~3mm/a; c) In the mid-southern plateau, there is a basin and endorheic subregion with a series of NE striking normal faults, showing obvious sinking with the rates between 0 to -4mm/a; d) The present-day rising and sinking subregions generally correspond well to the Cenozoic orogenic belts and basins, respectively; e) At the southeastern corner of the plateau, although the horizontal velocity field demonstrates an outstanding clockwise rotation and fan-like front of a flow zone, the vertical velocity field does not show a general uplift or incline trend. Horizontal GPS velocities of the Tibetan plateau relative to stable Eurasia Vertical GPS velocities of the Tibetan plateau relative to its stable northern neighbor

  18. A High-resolution 3D Geodynamical Model of the Present-day India-Asia Collision System

    NASA Astrophysics Data System (ADS)

    Kaus, B.; Baumann, T.

    2015-12-01

    We present a high-resolution, 3D geodynamic model of the present-day India-Asia collision system. The model is separated into multiple tectonic blocks, for which we estimate the first order rheological properties and the impact on the dynamics of the collision system. This is done by performing systematic simulations with different rheologies to minimize the misfit to observational constraints such as the GPS-velocity field. The simulations are performed with the parallel staggered grid FD code LaMEM using a numerical resolution of at least 512x512x256 cells to resolve dynamically important shear zones reasonably well. A fundamental part of this study is the reconstruction of the 3D present-day geometry of Tibet and the adjacent regions. Our interpretations of crust and mantle lithosphere geometry are jointly based on a globally available shear wave tomography (Schaeffer and Lebedev, 2013) and the Crust 1.0 model (Laske et al. http://igppweb.ucsd.edu/~gabi/crust1.html). We regionally refined and modified our interpretations based on seismicity distributions and focal mechanisms and incorporated regional receiver function studies to improve the accuracy of the Moho in particular. Results suggest that we can identify at least one "best-fit" solution in terms of rheological model properties that reproduces the observed velocity field reasonably well, including the strong rotation of the GPS velocity around the eastern syntax of the Himalaya. We also present model co-variances to illustrate the trade-offs between the rheological model parameters, their respective uncertainties, and the model fit. Schaeffer, A.J., Lebedev, S., 2013. Global shear speed structure of the upper mantle and transition zone. Geophysical Journal International 194, 417-449. doi:10.1093/gji/ggt095

  19. HLA Class I and Class II Alleles and Haplotypes Confirm the Berber Origin of the Present Day Tunisian Population

    PubMed Central

    Hajjej, Abdelhafidh; Almawi, Wassim Y.; Hattab, Lasmar; El-Gaaied, Amel; Hmida, Slama

    2015-01-01

    In view of its distinct geographical location and relatively small area, Tunisia witnessed the presence of many civilizations and ethnic groups throughout history, thereby questioning the origin of present-day Tunisian population. We investigated HLA class I and class II gene profiles in Tunisians, and compared this profile with those of Mediterranean and Sub-Sahara African populations. A total of 376 unrelated Tunisian individuals of both genders were genotyped for HLA class I (A, B) and class II (DRB1, DQB1), using reverse dot-blot hybridization (PCR-SSO) method. Statistical analysis was performed using Arlequin software. Phylogenetic trees were constructed by DISPAN software, and correspondence analysis was carried out by VISTA software. One hundred fifty-three HLA alleles were identified in the studied sample, which comprised 41, 50, 40 and 22 alleles at HLA-A,-B,-DRB1 and -DQB1 loci, respectively. The most frequent alleles were HLA-A*02:01 (16.76%), HLA-B*44:02/03 (17.82%), HLA-DRB1*07:01 (19.02%), and HLA-DQB1*03:01 (17.95%). Four-locus haplotype analysis identified HLA-A*02:01-B*50:01-DRB1*07:01-DQB1*02:02 (2.2%) as the common haplotype in Tunisians. Compared to other nearby populations, Tunisians appear to be genetically related to Western Mediterranean population, in particular North Africans and Berbers. In conclusion, HLA genotype results indicate that Tunisians are related to present-day North Africans, Berbers and to Iberians, but not to Eastern Arabs (Palestinians, Jordanians and Lebanese). This suggests that the genetic contribution of Arab invasion of 7th-11th century A.D. had little impact of the North African gene pool. PMID:26317228

  20. The effects of the host-substrate properties on maar-diatreme volcanoes: experimental evidence

    NASA Astrophysics Data System (ADS)

    Macorps, Élodie; Graettinger, Alison H.; Valentine, Greg A.; Ross, Pierre-Simon; White, James D. L.; Sonder, Ingo

    2016-04-01

    While the relationship between the host-substrate properties and the formation of maar-diatreme volcanoes have been investigated in the past, it remains poorly understood. In order to establish the effects of the qualitative host-substrate properties on crater depth, diameter, morphological features, and sub-surface structures, we present a comparison of four campaigns of experiments that used small chemical explosives buried in various geological media to simulate the formation of maar-diatremes. Previous results from these experiments have shown that primary variations in craters and sub-surface structures are related to the scaled depth (physical depth divided by cube root of blast energy). Our study reveals that single explosions at optimal scaled depths in stronger host materials create the largest and deepest craters with steep walls and the highest crater rims. For single explosions at deeper than optimal scaled depths, the influence of material strength is less obvious and non-linear for crater depth, and non-existent for crater diameter, within the range of the experiments. For secondary and tertiary blasts, there are no apparent relationships between the material properties and the crater parameters. Instead, the presence of pre-existing craters influences the crater evolution. A general weakening of the materials after successive explosions can be observed, suggesting a possible decrease in the host-substrate influence even at optimal scaled depth. The results suggest that the influence of the host-substrate properties is important only in the early stage of a maar-diatreme (neglecting post-eruptive slumping into the open crater) and decreases as explosion numbers increase. Since maar-diatremes reflect eruptive histories that involve tens to hundreds of individual explosions, the influence of initial substrate properties on initial crater processes could potentially be completely lost in a natural system.

  1. Complex Bedforms and Complex Water Masses: A Case Study from the Tertiary to Present-day, Pelotas Basin, Offshore Uruguay

    NASA Astrophysics Data System (ADS)

    Thompson, P.; Badalini, G.; Wrigley, S.; Walker, R.; Argent, J.; Hernandez-Molina, J.; de Santa Ana, H.; Soto, M.; Tomasini, J.

    2015-12-01

    varied both spatially and temporally and operated at various water depths. This complexity continues Present-day and is spectacularly imaged by pseudo time-lapse seismic data from the Present-day water column. These data, which are commonly neglected, highlight the true complexity of ocean currents and show how discrete dynamic water masses mix and move over time.

  2. Volcanoes and the environment: Lessons for understanding Earth's past and future from studies of present-day volcanic emissions

    NASA Astrophysics Data System (ADS)

    Mather, Tamsin A.

    2015-10-01

    Volcanism has affected the environment of our planet over a broad range of spatial (local to global) and temporal (< 1 yr to 100s Myr) scales and will continue to do so. As well as examining the Earth's geological record and using computer modelling to understand these effects, much of our knowledge of these processes comes from studying volcanism on the present-day planet. Understanding the full spectrum of possible routes and mechanisms by which volcanism can affect the environment is key to developing a realistic appreciation of possible past and potential future volcanic impact scenarios. This review paper seeks to give a synoptic overview of these potential mechanisms, focussing on those that we can seek to understand over human timescales by studying current volcanic activity. These effects are wide ranging from well-documented planetary-scale impacts (e.g., cooling by stratospheric aerosol veils) to more subtle or localised processes like ash fertilisation of ocean biota and impacts on cloud properties, atmospheric oxidant levels and terrestrial ecosystems. There is still much to be gained by studying present-day volcanic emissions. This review highlights the need for further work in three example areas. Firstly, to understand regional and arc-scale volcanic emissions, especially cycling of elements through subduction zones, more volatile measurements are needed to contribute to a fundamental and systematic understanding of these processes throughout geological time. Secondly, there is still uncertainty surrounding whether stratospheric ozone depletion following volcanic eruptions results solely from activation of anthropogenic halogen species. We should be poised to study future eruptions into the stratosphere with regard to their impacts and halogen load and work to improve our models and understanding of the relevant underlying processes within the Earth and the atmosphere. Thirdly, we lack a systematic understanding of trace metal volatility from magmas

  3. A novel GIS-based tool for estimating present-day ocean reference depth using automatically processed gridded bathymetry data

    NASA Astrophysics Data System (ADS)

    Jurecka, Mirosława; Niedzielski, Tomasz; Migoń, Piotr

    2016-05-01

    This paper presents a new method for computing the present-day value of the reference depth (dr) which is an essential input information for assessment of past sea-level changes. The method applies a novel automatic geoprocessing tool developed using Python script and ArcGIS, and uses recent data about ocean floor depth, sediment thickness, and age of oceanic crust. The procedure is multi-step and involves creation of a bathymetric dataset corrected for sediment loading and isostasy, delineation of subduction zones, computation of perpendicular sea-floor profiles, and statistical analysis of these profiles versus crust age. The analysis of site-specific situations near the subduction zones all around the world shows a number of instances where the depth of the oceanic crust stabilizes at a certain level before reaching the subduction zone, and this occurs at depths much lower than proposed in previous approaches to the reference depth issue. An analysis of Jurassic and Cretaceous oceanic lithosphere shows that the most probable interval at which the reference depth occurs is 5300-5800 m. This interval is broadly consistent with dr estimates determined using the Global Depth-Heatflow model (GDH1), but is significantly lower than dr estimates calculated on a basis of the Parsons-Sclater Model (PSM).

  4. MOCCA-SURVEY database I. Accreting white dwarf binary systems in globular clusters - I. Cataclysmic variables - present-day population

    NASA Astrophysics Data System (ADS)

    Belloni, Diogo; Giersz, Mirek; Askar, Abbas; Leigh, Nathan; Hypki, Arkadiusz

    2016-11-01

    In this paper, which is the first in a series of papers associated with cataclysmic variables and related objects, we introduce the CATUABA code, a numerical machinery written for analysis of the MOCCA simulations, and show some first results by investigating the present-day population of cataclysmic variables in globular clusters. Emphasis was given on their properties and the observational selection effects when observing and detecting them. In this work, we analysed in this work six models, including three with Kroupa distributions of the initial binaries. We found that for models with Kroupa initial distributions, considering the standard value of the efficiency of the common envelope phase adopted in BSE, no single cataclysmic variable was formed only via binary stellar evolution, i.e. in order to form them, strong dynamical interactions have to take place. We show and explain why this is inconsistent with observational and theoretical results. Our results indicate that the population of cataclysmic variables in globular clusters is, mainly, in the last stage of their evolution and observational selection effects can drastically change the expected number of observed cataclysmic variables. We show that the probability of observing them during the outbursts is extremely small and conclude that the best way of looking for cataclysmic variables in globular clusters is by searching for variabilities during quiescence, instead of during outbursts. For that, one would need a very deep observation which could reach magnitudes ≳27 mag. Finally, we argue that cataclysmic variables in globular clusters are not necessarily magnetic.

  5. Present-day genetic structure of Atlantic salmon (Salmo salar) in Icelandic rivers and ice-cap retreat models.

    PubMed

    Olafsson, Kristinn; Pampoulie, Christophe; Hjorleifsdottir, Sigridur; Gudjonsson, Sigurdur; Hreggvidsson, Gudmundur O

    2014-01-01

    Due to an improved understanding of past climatological conditions, it has now become possible to study the potential concordance between former climatological models and present-day genetic structure. Genetic variability was assessed in 26 samples from different rivers of Atlantic salmon in Iceland (total of 2,352 individuals), using 15 microsatellite loci. F-statistics revealed significant differences between the majority of the populations that were sampled. Bayesian cluster analyses using both prior information and no prior information on sampling location revealed the presence of two distinguishable genetic pools - namely, the Northern (Group 1) and Southern (Group 2) regions of Iceland. Furthermore, the random permutation of different allele sizes among allelic states revealed a significant mutational component to the genetic differentiation at four microsatellite loci (SsaD144, Ssa171, SSsp2201 and SsaF3), and supported the proposition of a historical origin behind the observed variation. The estimated time of divergence, using two different ABC methods, suggested that the observed genetic pattern originated from between the Last Glacial Maximum to the Younger Dryas, which serves as additional evidence of the relative immaturity of Icelandic fish populations, on account of the re-colonisation of this young environment following the Last Glacial Maximum. Additional analyses suggested the presence of several genetic entities which were likely to originate from the original groups detected. PMID:24498283

  6. Present-Day Atmospheric Simulations Using GISS ModelE: Comparison to In Situ, Satellite, and Reanalysis Data.

    NASA Astrophysics Data System (ADS)

    Schmidt, Gavin A.; Ruedy, Reto; Hansen, James E.; Aleinov, Igor; Bell, Nadine; Bauer, Mike; Bauer, Susanne; Cairns, Brian; Canuto, Vittorio; Cheng, Ye; Del Genio, Anthony; Faluvegi, Greg; Friend, Andrew D.; Hall, Tim M.; Hu, Yongyun; Kelley, Max; Kiang, Nancy Y.; Koch, Dorothy; Lacis, Andy A.; Lerner, Jean; Lo, Ken K.; Miller, Ron L.; Nazarenko, Larissa; Oinas, Valdar; Perlwitz, Jan; Perlwitz, Judith; Rind, David; Romanou, Anastasia; Russell, Gary L.; Sato, Makiko; Shindell, Drew T.; Stone, Peter H.; Sun, Shan; Tausnev, Nick; Thresher, Duane; Yao, Mao-Sung

    2006-01-01

    A full description of the ModelE version of the Goddard Institute for Space Studies (GISS) atmospheric general circulation model (GCM) and results are presented for present-day climate simulations (ca. 1979). This version is a complete rewrite of previous models incorporating numerous improvements in basic physics, the stratospheric circulation, and forcing fields. Notable changes include the following: the model top is now above the stratopause, the number of vertical layers has increased, a new cloud microphysical scheme is used, vegetation biophysics now incorporates a sensitivity to humidity, atmospheric turbulence is calculated over the whole column, and new land snow and lake schemes are introduced. The performance of the model using three configurations with different horizontal and vertical resolutions is compared to quality-controlled in situ data, remotely sensed and reanalysis products. Overall, significant improvements over previous models are seen, particularly in upper-atmosphere temperatures and winds, cloud heights, precipitation, and sea level pressure. Data model comparisons continue, however, to highlight persistent problems in the marine stratocumulus regions.

  7. New constraints on the present-day kinematics of the East African Rift from GPS and earthquake slip vector data

    NASA Astrophysics Data System (ADS)

    Hartnady, C.; Calais, E.; Ebinger, C.; Nocquet, J.

    2004-12-01

    The East African Rift (EAR), a major 5,000 km long and up to 1,000 km wide tectonic structure that marks the extensional boundary between the Nubian and Somalian plate, is interpreted either as a wide zone of uniformly distributed, diffuse deformation, or as a mosaic of microplates. Testing these models and quantifying the present-day kinematics of the EAR has so far resited investigation because of a critical lack of geodetic data within the EAR as well as on the surrounding Nubian and Somalian plates. Here, we present an updated GPS velocity field covering the Nubian and Somalian plates and combine it with earthquake slip vectors along the EAR in a joint inversion. Our objectives are to better constrain the Somalia/Nubia plate motion and to try to resolve block motions within the plate boundary zone. We find a Somalia/Nubia angular velocity similar to the one proposed by Fernandes et al. (EPSL, 222, 2004). We show that Tanzanian craton, nested between the western and eastern branches of the EAR and underlained by an upper mantle plume, can be modeled as an independent block, rotating counterclockwise w.r.t. Nubia. We discuss the implications of this kinematic model on the tectonics of the EAR.

  8. Atmospheric transport of persistent organic pollutants to and from the Arctic under present-day and future climate.

    PubMed

    Octaviani, Mega; Stemmler, Irene; Lammel, Gerhard; Graf, Hans F

    2015-03-17

    The long-term atmospheric cycling and fate of persistent organic pollutants under the influence of a changing climate is a concern. A GCM's realization of present-day (1970-1999) and future (2070-2099) climate, the latter under a medium scenario of greenhouse gas emissions, is used to study meridional transports and their correlations with the Arctic and North Atlantic Oscillations (AO and NAO). Regions of import and export maxima into the Arctic are identified along the Arctic Circle. It is found that, under future climate conditions, the net export of PCB153 out of the Arctic will increase. The meridional net flux pattern of this substance is expected to become independent of AO and NAO. For DDT, a trend of decreasing net Arctic import will reverse to an increasing trend 100 years after peak emission, which is partly due to more frequent AO and NAO positive phases. It is concluded that the long-term accumulation trends in the Arctic of other persistent pollutants, including so-called emerging pollutants, are subject to the substances' specific behavior and fate in the environment and need to be studied specifically. PMID:25686012

  9. Present-Day Crustal Vertical Motion Around the Ordos Block Constrained by Precise Leveling and GPS Data

    NASA Astrophysics Data System (ADS)

    Hao, Ming; Wang, Qingliang; Cui, Duxin; Liu, Liwei; Zhou, Lin

    2016-09-01

    Precise leveling data observed in the period of 1970-2014 around the Ordos block were collected and processed to estimate present-day crustal vertical movement. Vertical rates of 6 GPS sites were employed as a priori constraints to define the reference frame. The velocity field shows that the interior of the Ordos block moves upward at a rate of 3 mm/a as a stable block. With respect to the central Ordos, the grabens and rifts around the Ordos block are undergoing subsidence, while the northeastern and southwestern Ordos uplift at the average rates of 2 and 1 mm/a, respectively. To the southeastern margin of the Ordos block, the Weihe and southern Shanxi grabens are subsiding at the rates of 4-6 mm/a. The subsidence of the Shanxi graben indicates that the graben is experiencing extensional movement on a long timescale. To the northwestern margin of the Ordos block, the Hetao and Yinchuan rifts are subsiding at the rates of 2-3 mm/a. A 2-D buried faulting model is used to infer the normal or reverse dip-slip rates. Our solution shows that most of the normal slip rates along the faults in the grabens and rifts are ~2 mm/a.

  10. Global connections between aeolian dust, climate and ocean biogeochemistry at the present day and at the last glacial maximum

    NASA Astrophysics Data System (ADS)

    Maher, B. A.; Prospero, J. M.; Mackie, D.; Gaiero, D.; Hesse, P. P.; Balkanski, Y.

    2010-04-01

    Palaeo-dust records in sediments and ice cores show that wind-borne mineral aerosol ('dust') is strongly linked with climate state. During glacial climate stages, for example, the world was much dustier, with dust fluxes two to five times greater than in interglacial stages. However, the influence of dust on climate remains a poorly quantified and actively changing element of the Earth's climate system. Dust can influence climate directly, by the scattering and absorption of solar and terrestrial radiation, and indirectly, by modifying cloud properties. Dust transported to the oceans can also affect climate via ocean fertilization in those regions of the world's oceans where macronutrients like nitrate are abundant but primary production and nitrogen fixation are limited by iron scarcity. Dust containing iron, as fine-grained iron oxides/oxyhydroxides and/or within clay minerals, and other essential micronutrients (e.g. silica) may modulate the uptake of carbon in marine ecosystems and, in turn, the atmospheric concentration of CO 2. Here, in order to critically examine past fluxes and possible climate impacts of dust in general and iron-bearing dust in particular, we consider present-day sources and properties of dust, synthesise available records of dust deposition at the last glacial maximum (LGM); evaluate the evidence for changes in ocean palaeo-productivity associated with, and possibly caused by, changes in aeolian flux to the oceans at the LGM; and consider the radiative forcing effects of increased LGM dust loadings.

  11. Model sensitivity of ice flux over the grounding line to present-day climatic forcing and geothermal flux

    NASA Astrophysics Data System (ADS)

    Kleiner, Thomas; Humbert, Angelika

    2016-04-01

    Large uncertainties remain in the current and future contribution to sea level change from Antarctica from observations and numerical flow modelling. Within the SeaRISE project atmospheric, oceanic, and subglacial forcing scenarios were applied to different ice-sheet models to assess Antarctic ice sheet sensitivity over a 500 year timescale. The scenario results have been compared to the individual state of each model at the end of its spin-up. It has been shown, that the model results highly depend on the chosen climate forcing and spin-up strategy. Here we use the Parallel Ice Sheet Model (PISM) to perform spin-up simulations across different data sets for present-day boundary conditions for the Antarctic Ice Sheet (surface temperature, surface mass balance and geothermal flux). The utilized spin-up methods include free evolving and geometry constrained simulations. Here we present our analysis of the ice flux over the grounding line for each set-up and compare the fluxes from large drainage basin units with estimates derived from remote sensing.

  12. Present-day stress field on the South American slab underneath the Sandwich Plate (Southern Atlantic Ocean)

    NASA Astrophysics Data System (ADS)

    Giner-Robles, J. L.; Pérez-López, R.; Álvarez-Gómez, J. A.; Martínez-Díaz, J. J.; Rodríguez-Pascua, M. A.

    2009-04-01

    This work confirms the present-day principal stress orientation on the South Sandwich Plate (SSP) from the analysis of 331 earthquake focal mechanisms (Harvard catalog, HCMT). Principal stress orientation was deduced from earthquake focal mechanisms, examined by fault population analysis methods. The SSP plate is composed by oceanic crust limits an elliptical trench to the east (South Sandwich Trench), a ridge to the west and transforms faults towards the northern and southern boundaries. Within the trench region, the maximum horizontal shortening direction (SHMAX) rotates in trend in a clockwise direction, from NNE, in the northern boundary, to SSE in the southern boundary. Therefore, and keeping in mind the gradual rotation of SHMAX along the trench, three different areas were defined according to the prevailing focal mechanism type: (1) the North Zone, with SHMAX oriented N060°E and reverse and strike-slip focal mechanisms; (2) the Central Zone, with only reverse focal mechanism and SHMAX striking N080°E; (3) the South Zone, with SHMAX oriented N110°E and reverse and strike-slip focal geometry. Furthermore, the accommodation of the strain field in the Northern Zone of the South Sandwich Plate generates a subduction decoupling of the slab at, approximately, 70 km depth. In contrast, the South Zone slab exhibits a gradual stress and strain magnitude decreasing in depth. Finally, we define a sinistral strike-slip parallel to the southern boundary between the South Sandwich Plate and the Antarctic Plate, the South Sandwich Fault Zone.

  13. Evaluation of Present-day Aerosols over China Simulated from the Atmospheric Chemistry and Climate Model Intercomparison Project (ACCMIP)

    NASA Astrophysics Data System (ADS)

    Liao, H.; Chang, W.

    2014-12-01

    High concentrations of aerosols over China lead to strong radiative forcing that is important for both regional and global climate. To understand the representation of aerosols in China in current global climate models, we evaluate extensively the simulated present-day aerosol concentrations and aerosol optical depth (AOD) over China from the 12 models that participated in Atmospheric Chemistry & Climate Model Intercomparison Project (ACCMIP), by using ground-based measurements and satellite remote sensing. Ground-based measurements of aerosol concentrations used in this work include those from the China Meteorological Administration (CMA) Atmosphere Watch Network (CAWNET) and the observed fine-mode aerosol concentrations collected from the literature. The ground-based measurements of AOD in China are taken from the AErosol RObotic NETwork (AERONET), the sites with CIMEL sun photometer operated by Institute of Atmospheric Physics, Chinese Academy of Sciences, and from Chinese Sun Hazemeter Network (CSHNET). We find that the ACCMIP models generally underestimate concentrations of all major aerosol species in China. On an annual mean basis, the multi-model mean concentrations of sulfate, nitrate, ammonium, black carbon, and organic carbon are underestimated by 63%, 73%, 54%, 53%, and 59%, respectively. The multi-model mean AOD values show low biases of 20-40% at studied sites in China. The ACCMIP models can reproduce seasonal variation of nitrate but cannot capture well the seasonal variations of other aerosol species. Our analyses indicate that current global models generally underestimate the role of aerosols in China in climate simulations.

  14. Atmospheric transport of persistent organic pollutants to and from the Arctic under present-day and future climate.

    PubMed

    Octaviani, Mega; Stemmler, Irene; Lammel, Gerhard; Graf, Hans F

    2015-03-17

    The long-term atmospheric cycling and fate of persistent organic pollutants under the influence of a changing climate is a concern. A GCM's realization of present-day (1970-1999) and future (2070-2099) climate, the latter under a medium scenario of greenhouse gas emissions, is used to study meridional transports and their correlations with the Arctic and North Atlantic Oscillations (AO and NAO). Regions of import and export maxima into the Arctic are identified along the Arctic Circle. It is found that, under future climate conditions, the net export of PCB153 out of the Arctic will increase. The meridional net flux pattern of this substance is expected to become independent of AO and NAO. For DDT, a trend of decreasing net Arctic import will reverse to an increasing trend 100 years after peak emission, which is partly due to more frequent AO and NAO positive phases. It is concluded that the long-term accumulation trends in the Arctic of other persistent pollutants, including so-called emerging pollutants, are subject to the substances' specific behavior and fate in the environment and need to be studied specifically.

  15. Experimental approaches to investigate effector translocation into host cells in the Ustilago maydis/maize pathosystem.

    PubMed

    Tanaka, Shigeyuki; Djamei, Armin; Presti, Libera Lo; Schipper, Kerstin; Winterberg, Sarah; Amati, Simone; Becker, Dirk; Büchner, Heike; Kumlehn, Jochen; Reissmann, Stefanie; Kahmann, Regine

    2015-01-01

    The fungus Ustilago maydis is a pathogen that establishes a biotrophic interaction with Zea mays. The interaction with the plant host is largely governed by more than 300 novel, secreted protein effectors, of which only four have been functionally characterized. Prerequisite to examine effector function is to know where effectors reside after secretion. Effectors can remain in the extracellular space, i.e. the plant apoplast (apoplastic effectors), or can cross the plant plasma membrane and exert their function inside the host cell (cytoplasmic effectors). The U. maydis effectors lack conserved motifs in their primary sequences that could allow a classification of the effectome into apoplastic/cytoplasmic effectors. This represents a significant obstacle in functional effector characterization. Here we describe our attempts to establish a system for effector classification into apoplastic and cytoplasmic members, using U. maydis for effector delivery.

  16. Temporal autocorrelation in host density increases establishment success of parasitoids in an experimental system

    PubMed Central

    Vercken, Elodie; Fauvergue, Xavier; Ris, Nicolas; Crochard, Didier; Mailleret, Ludovic

    2015-01-01

    Environmental variation is classically expected to affect negatively population growth and to increase extinction risk, and it has been identified as a major determinant of establishment failures in the field. Yet, recent theoretical investigations have shown that the structure of environmental variation and more precisely the presence of positive temporal autocorrelation might alter this prediction. This is particularly likely to affect the establishment dynamics of biological control agents in the field, as host–parasitoid interactions are expected to induce temporal autocorrelation in host abundance. In the case where parasitoid populations display overcompensatory dynamics, the presence of such positive temporal autocorrelation should increase their establishment success in a variable environment. We tested this prediction in laboratory microcosms by introducing parasitoids to hosts whose abundances were manipulated to simulate uncorrelated or positively autocorrelated variations in carrying capacity. We found that environmental variability decreased population size and increased parasitoid population variance, which is classically expected to extinction risk. However, although exposed to significant environmental variation, we found that parasitoid populations experiencing positive temporal autocorrelation in host abundance were more likely to persist than populations exposed to uncorrelated variation. These results confirm that environmental variation is a key determinant of extinction dynamics that can have counterintuitive effects depending on its autocorrelation structure. PMID:26257880

  17. Present-day deformation in NE Iran and the South Caspian constraint by Global Positioning System measurements

    NASA Astrophysics Data System (ADS)

    Mousavi, Z.; Walpersdorf, A.; Walker, R. T.; Tavakoli, F.; Pathier, E.; Nankali, H.; Nilfouroushan, F.; Aghamohammadi, A.; Djamour, Y.

    2012-12-01

    The continental collision between Arabia, the Eurasia and distribution of earthquake epicenters show that most of the deformation is accommodated within the political borders of Iran. In recent years, constraints from GPS, seismology and geological estimates of fault slip-rate have allowed considerable advances in understanding the rates and kinematics of faulting across many parts of Iran. However, until now, only little is known on the present-day distribution of strain across the eastern and northeastern parts of the country, such that it has been difficult to assess the rates of faulting, the related earthquake hazard, and the relationship between the active faults and the overall tectonic motions. This area is one of the most densely populated regions of Iran with almost 6.5 million habitants and a significant number of historical earthquakes like the Qumis 856 A.D earthquake with 200.000 victims. But while eastern Alborz and Kopeh Dagh are clearly regions of active faulting, a lack of instrumental earthquakes is presently observed, making this area particularly interesting for hazard assessment studies. The sparse GPS measurements in NE Iran provide only limited constraints on the applicability of different kinematic scenarios that have been proposed to explain the role of the observed faults. Here, we present a velocity field, composed from 47 GPS stations (20 campaign and 27 permanent), recording over up to 11 years, and covering the entire NE of Iran. This new GPS velocity field helps to investigate how northward directed Arabia-Eurasia shortening is accommodated at the northern boundary of the deforming zone. A regional deformation field for NE Iran has been estimated from the GPS measurements. It shows how the incoming ~7 mm/yr of NS shortening between Central Iran and Eurasia is accommodated in Alborz, Binalud and Kopeh Dagh. The shortening rate decreases toward the east and dies out at the Afghanistan border. The deformation pattern is contrasted along

  18. The Herschel view of the dominant mode of galaxy growth from z = 4 to the present day

    NASA Astrophysics Data System (ADS)

    Schreiber, C.; Pannella, M.; Elbaz, D.; Béthermin, M.; Inami, H.; Dickinson, M.; Magnelli, B.; Wang, T.; Aussel, H.; Daddi, E.; Juneau, S.; Shu, X.; Sargent, M. T.; Buat, V.; Faber, S. M.; Ferguson, H. C.; Giavalisco, M.; Koekemoer, A. M.; Magdis, G.; Morrison, G. E.; Papovich, C.; Santini, P.; Scott, D.

    2015-03-01

    our findings on the cosmic SFR history and on the origin of present-day stars: more than two-thirds of present-day stars must have formed in a regime dominated by the "main sequence" mode. As a consequence we conclude that, although omnipresent in the distant Universe, galaxy mergers had little impact in shaping the global star formation history over the last 12.5 billion years. Herschel is an ESA space observatory with science instruments provided by European-led Principal Investigator consortia and with important participation from NASA.Appendices are available in electronic form at http://www.aanda.org

  19. Spatial distribution of precipitation annual cycles over South Africa in 10 CORDEX regional climate model present-day simulations

    NASA Astrophysics Data System (ADS)

    Favre, Alice; Philippon, Nathalie; Pohl, Benjamin; Kalognomou, Evangelia-Anna; Lennard, Christopher; Hewitson, Bruce; Nikulin, Grigori; Dosio, Alessandro; Panitz, Hans-Juergen; Cerezo-Mota, Ruth

    2016-03-01

    This study presents an evaluation of the ability of 10 regional climate models (RCMs) participating in the COordinated Regional climate Downscaling Experiment-Africa to reproduce the present-day spatial distribution of annual cycles of precipitation over the South African region and its borders. As found in previous studies, annual mean precipitation is quasi-systematically overestimated by the RCMs over a large part of southern Africa south of about 20°S and more strongly over South Africa. The spatial analysis of precipitation over the studied region shows that in most models the distribution of biases appears to be linked to orography. Wet biases are quasi-systematic in regions with higher elevation with inversely neutral to dry biases particularly in the coastal fringes. This spatial pattern of biases is particularly obvious during summer and specifically at the beginning of the rainy season (November and December) when the wet biases are found to be the strongest across all models. Applying a k-means algorithm, a classification of annual cycles is performed using observed precipitation data, and is compared with those derived from modeled data. It is found that the in-homogeneity of the spatial and temporal distribution of biases tends to impact the modeled seasonality of precipitation. Generally, the pattern of rainfall seasonality in the ensemble mean of the 10 RCMs tends to be shifted to the southwest. This spatial shift is mainly linked to a strong overestimation of convective precipitation at the beginning of the rainy season over the plateau inducing an early annual peak and to an underestimation of stratiform rainfall in winter and spring over southwestern South Africa.

  20. Multivariate spatio-temporal modelling for assessing Antarctica's present-day contribution to sea-level rise

    PubMed Central

    Zammit-Mangion, Andrew; Rougier, Jonathan; Schön, Nana; Lindgren, Finn; Bamber, Jonathan

    2015-01-01

    Antarctica is the world's largest fresh-water reservoir, with the potential to raise sea levels by about 60 m. An ice sheet contributes to sea-level rise (SLR) when its rate of ice discharge and/or surface melting exceeds accumulation through snowfall. Constraining the contribution of the ice sheets to present-day SLR is vital both for coastal development and planning, and climate projections. Information on various ice sheet processes is available from several remote sensing data sets, as well as in situ data such as global positioning system data. These data have differing coverage, spatial support, temporal sampling and sensing characteristics, and thus, it is advantageous to combine them all in a single framework for estimation of the SLR contribution and the assessment of processes controlling mass exchange with the ocean. In this paper, we predict the rate of height change due to salient geophysical processes in Antarctica and use these to provide estimates of SLR contribution with associated uncertainties. We employ a multivariate spatio-temporal model, approximated as a Gaussian Markov random field, to take advantage of differing spatio-temporal properties of the processes to separate the causes of the observed change. The process parameters are estimated from geophysical models, while the remaining parameters are estimated using a Markov chain Monte Carlo scheme, designed to operate in a high-performance computing environment across multiple nodes. We validate our methods against a separate data set and compare the results to those from studies that invariably employ numerical model outputs directly. We conclude that it is possible, and insightful, to assess Antarctica's contribution without explicit use of numerical models. Further, the results obtained here can be used to test the geophysical numerical models for which in situ data are hard to obtain. © 2015 The Authors. Environmetrics published by John Wiley & Sons Ltd. PMID:25937792

  1. The Secrets of the Nearest Starburst Cluster. II. The Present-Day Mass Function in NGC 3603

    NASA Astrophysics Data System (ADS)

    Stolte, Andrea; Brandner, Wolfgang; Brandl, Bernhard; Zinnecker, Hans

    2006-07-01

    Based on deep Very Large Telescope Infrared Spectrometer and Array Camera JHK photometry, we have derived the present-day mass function (MF) of the central starburst cluster NGC 3603 YC (Young Cluster) in the giant H II region NGC 3603. The effects of field contamination, individual reddening, and a possible binary contribution are investigated. The MF slopes resulting from the different methods are compared and lead to a surprisingly consistent cluster MF with a slope of Γ=-0.9+/-0.15. Analyzing different radial annuli around the cluster core, no significant change in the slope of the MF is observed. However, mass segregation in the cluster is evidenced by the increasing depletion of the high-mass tail of the stellar mass distribution with increasing radius. We discuss the indications of mass segregation with respect to the changes observed in the binned and cumulative stellar MFs and argue that the cumulative function, as well as the fraction of high- to low-mass stars, provides better indicators for mass segregation than the MF slope alone. Finally, the observed MF and starburst morphology of NGC 3603 YC are discussed in the context of massive local star-forming regions such as the Galactic center Arches cluster, R136/30 Dor in the LMC, and the Orion Trapezium cluster, all providing resolved templates for extragalactic star formation. Despite the similarity in the observed MF slopes, dynamical considerations suggest that the starburst clusters do not form gravitationally bound systems over a Hubble time. Both the environment (gravitational potential of the Milky Way) and the concentration of stars in the cluster core determine the dynamical stability of a dense star cluster, such that the long-term evolution of a starburst is not exclusively determined by the stellar evolution of its members, as frequently assumed for globular cluster systems. Based on observations obtained at the ESO Very Large Telescope on Paranal, Chile, under programs 63.I-0015 and 65.I

  2. Lyman Alpha-emitting Galaxies at z = 2.1: Characterizing the Progenitors of Typical Present-day Galaxies

    NASA Astrophysics Data System (ADS)

    Gawiser, Eric J.; Guaita, L.; Padilla, N.; Francke, H.; Bond, N. A.; Gronwall, C.; Ciardullo, R.; Sinawa, S.; Feldmeier, J. J.; MUSYC Collaboration

    2010-01-01

    We discovered a sample of 261 Lyman alpha emitting (LAE) galaxies at z=2.1 in an ultra-deep 3727A narrow-band MUSYC image of the Extended Chandra Deep Field-South. LAEs were selected to have rest-frame equivalent widths >20A and emission line fluxes >3.7x10-17 ergs/cm2/s, corresponding to L_Lya>1.2x1042 ergs/s. 3% of the original candidates were detected in X-rays by Chandra, and 7% were detected in the rest-frame far-UV by GALEX; these objects were eliminated to minimize contamination by AGN and low-redshift galaxies. Our sample has median rest-frame EW=40A, and only a few galaxies have rest-frame EW bigger than 200A. Our results show that the luminosity function of LAEs at z=2.1 is consistent with that of LAEs at z=3.1 but with number density a factor of 1.8+-0.3 higher. We used the rest frame UV luminosity to estimate a median star formation rate of 4 Msun/yr. Clustering analysis reveals that LAEs at z=2.1 have r0=3+-0.5 Mpc, corresponding to b=1.0+-0.2, the lowest clustering bias of any high-redshift galaxy population. This implies that z=2.1 LAEs reside in dark matter halos with masses 1010 Msun, which are the lowest-mass halos yet probed at this redshift. We used the Sheth-Tormen conditional mass function to study the descendants of these LAEs and found that their typical present-day descendants are local galaxies with sub-L* and L* luminosities, like the Milky Way. We gratefully acknowledge grant support for this research from NSF, DOE, and NASA.

  3. Impact of the Yakutat indentor corner on present-day tectonics and fault activity in SE Alaska - SW Yukon

    NASA Astrophysics Data System (ADS)

    Mazzotti, S.; Marechal, A.; Ritz, J. F.; Ferry, M. A.

    2015-12-01

    We present an active tectonic model of the SE Alaska - SW Yukon region based principally on the integration of recent GPS velocity data and new fault-slip rates derived from geomorphology. In this region, the Yakutat collision results in complex tectonics with patterns of strain localization and strain partitioning that strongly vary across the various mountain ranges and active faults. We propose that deformation and fault activity in the St. Elias and Chugach Mountains are primarily controlled by the eastern syntaxis of the Yakutat collision, which produces a semi-radial tectonic pattern: Velocities, principal horizontal shortening rates, and maximum horizontal stress orientations rotate by 60 - 80 ° around the syntaxis, from roughly parallel to the relative Pacific - North America motion at the front of the collision to roughly orthogonal southeast of the syntaxis. The interaction between this strain pattern and major inherited tectonic structures inland of the collision zone (i.e., Denali and Duke River Faults) results in various reactivation modes of these structures. Specifically, the Denali Fault shows a very pronounced lateral variations of activity from ~12 mm/a of dextral slip rate in its central section to ~1 mm/a of mostly shortening slip rate along its southern section. This marked change of activity is associated with a possible relay system where the Duke River and Totschunda Faults accommodate a major part (8 - 12 mm/a) of the inland strain transfer directly in front of the syntaxis. This new tectonic model retains some questions, in particular regarding the mechanisms of deformation and strain transfer (1) from the syntaxis to the Duke River - Totschunda system and (2) at the junction between Totschunda and Denali Faults. Numerical models of present-day deformation may help address these issues and provide information about relative strength of the various crustal and inherited fault elements of this system.

  4. Present-day crustal deformation along the Magallanes-Fagnano Fault System in Tierra del Fuego from repeated GPS observations

    NASA Astrophysics Data System (ADS)

    Mendoza, L.; Perdomo, R.; Hormaechea, J. L.; Del Cogliano, D.; Fritsche, M.; Richter, A.; Dietrich, R.

    2011-03-01

    The present-day deformation of the earth crust in the Argentine part of Tierra del Fuego main island (southernmost South America) is here investigated based on repeated geodetic GPS observations. The island is traversed by the active transform boundary between the South American and Scotia tectonic plates, represented by the Magallanes-Fagnano fault system. Since 1993 a regional network comprising to date 29 GPS sites has been observed almost every year. The complete set of accumulated observations was processed using the Bernese GPS software and state-of-the-art processing strategies and models. The utilization of homogeneous GPS products resulting from a reprocessing of the global IGS network warrants a stable realization of a global reference frame. For each GPS site 3-D positions and linear velocities with error estimates were obtained. A strain analysis of the horizontal velocity components revealed the zones of major deformation activity. A 30-km-wide deformation belt centred on the main trace of the fault system was identified. This belt is bordered to the north (South America) and south (Scotia) by geodynamically stable zones, which move horizontally with a relative average velocity of 4.4 ± 0.6 (east) and -0.3 ± 0.4 (north) mm a-1. Within the deformation belt a maximum strain rate in the order of 0.25 μstrain per year has been detected. A pronounced change in the deformation style from transtension (east) to transpression (west) is observed. The area of predominating shortening of the crust coincides with a local rotation minimum and relative uplift. Throughout the period covered by the GPS observations the displacements and deformations occurred to be linear with time.

  5. Historical and Present Day Mercury Contamination From Gold Mining in Three Feeding Guilds of Bats From the Peruvian Amazon

    NASA Astrophysics Data System (ADS)

    Kumar, A.; Divoll, T.

    2014-12-01

    Miners in many countries use mercury as an amalgam to separate gold from river sediments. In the last twenty years the price of gold has risen and the number of small-scale, artisanal gold mining operations in the Amazon basin have also increased. The influx of mercury into natural river systems has detrimental consequences for the surrounding ecosystem and for organisms, particularly those at higher trophic levels. Toxic mercury levels have been shown to impair reproductive, neurological and behavioral functioning of organisms. I used bats (Chiroptera) as a mammalian model system to study mercury contamination and accumulation due to gold mining from field caught and museum collection specimens in Amazonian Perú and showed that: (1) Total mercury concentrations in Amazonian bat species have increased over time since the 1920's; (2) Bat species from sites with current active mining have higher concentrations of mercury than non-mining sites, with some species having levels exceeding those considered toxic for mammals; (3) Higher trophic levels of bats (piscivores and insectivores) bioaccumulate more mercury than bats of lower trophic levels (frugivores); (4) Bats located in present day uncontaminated sites have the same mercury levels as bats collected in the 1920's from the Amazon basin. The variety of bat feeding guilds allowed for a comparison of how mercury accumulation is affected by diet within one taxonomic order. The novel use of museum specimens allowed for a look back into the historical timeline of mercury contamination in the Amazon basin. Bats represent a new and exciting study system since, like humans, they are mammals and should therefore show similar neurochemical and behavioral responses to this toxic element.

  6. Life detection at a Mars analogue site of present-day serpentinization in the Tablelands Ophiolite of Newfoundland (Invited)

    NASA Astrophysics Data System (ADS)

    Morrill, P. L.; Szponar, N.; Brazelton, W. J.; Woodruff, Q.; Schrenk, M. O.; Bower, D. M.; Steele, A.

    2010-12-01

    The Tableland Ophiolite was created during the collision of Laurentia and Gondwana continents ca. 470 million years ago. Ultramafic mantle rocks, from the ancient sea bed that once separated these continents, were thrusted westward onto the old continental margin, which is now Western Newfoundland. Weathering due to recent glaciations has left large areas of ultramafic rock at the surface and created fissures for fluid flow. As a result serpentinization is occurring as fresh water penetrates the unaltered ultramafic rock. Serpentinization is of particular interest because, through hydration of ultramafic rock, this reaction produces H2 and the reducing conditions necessary for abiogenic hydrocarbon synthesis, while also producing conditions amenable for chemolithotrophic life. Therefore sites of active serpentinization can be the source of either abiogenic or biogenic organics, or both. Serpentinization is a suspected (past or present) source of (detected or putative) hydrocarbons on Mars, Titan and Europa, hence these astrobodies may be potentially habitable or once habitable environments. The Tablelands Ophiolite is an analogue site that is ideal for testing methods of life detection in an extreme environment of high pH and low microbial biomass characteristic of sites of serpentinization. Multiple ultrabasic reducing springs characteristic of present-day serpentinization have been identified and characterized based on their geochemistry and microbiology. Field-based instruments were deployed for the detection of microbial activity (ATP), microbial cell wall material, and mineralogy, in yet untested high pH and low biomass environment. In this talk I will give an overview of the in situ measurements of life detection and put these measurements in context of geochemistry, microbiology, carbon source and reaction pathways, and I will discuss what we have learned that will help us plan for future mission measurements.

  7. Present-day kinematics and fault slip rates in eastern Iran, derived from 11 years of GPS data

    NASA Astrophysics Data System (ADS)

    Walpersdorf, A.; Manighetti, I.; Mousavi, Z.; Tavakoli, F.; Vergnolle, M.; Jadidi, A.; Hatzfeld, D.; Aghamohammadi, A.; Bigot, A.; Djamour, Y.; Nankali, H.; Sedighi, M.

    2014-02-01

    We analyze new GPS data spanning 11 years at 92 stations in eastern Iran. We use these data to analyze the present-day kinematics and the slip rates on most seismogenic faults in eastern Iran. The east Lut, west Lut, Kuhbanan, Anar, Dehshir, and Doruneh faults are confirmed as the major faults and are found to currently slip laterally at 5.6 ± 0.6, 4.4 ± 0.4, 3.6 ± 1.3, 2.0 ± 0.7, 1.4 ± 0.9, and 1.3 ± 0.8 mm/yr, respectively. Slip is right-lateral on the ~NS striking east Lut, west Lut, Kuhbanan, Anar, and Dehshir faults and left-lateral on the ~EW Doruneh fault. The ~NS faults slice the eastern Iranian crust into five blocks that are moving northward at 6-13 mm/yr with respect to the stable Afghan crust at the eastern edge of the collision zone. The collective behavior of the ~NS faults might thus allow the Arabian promontory to impinge northward into the Eurasian crust. The ~NS faults achieve additional NS shortening by rotating counterclockwise in the horizontal plane, at current rates up to 0.8°/Ma. Modeling the GPS and available geological data with a block rotation model suggests that the rotations have been going on at a similar rate (1 ± 0.4°/Ma) over the last 12 Ma. We identify large strains at the tips of the rotating east Lut, west Lut, and Kuhbanan faults, which we suspect to be responsible for the important historical and instrumental seismicity in those zones.

  8. Reverse-current effect in present-day models of solar flares: Theory and high-accuracy observations

    NASA Astrophysics Data System (ADS)

    Gritsyk, P. A.; Somov, B. V.

    2014-08-01

    We propose an accurate analytical model for the source of hard X-ray emission from a flare in the form of a "thick target" with a reverse current to explain the results of present-day observations of solar flares onboard the GOES, Hinode, RHESSI, and TRACE satellites. The model, one-dimensional in coordinate space and two-dimensional in velocity space, self-consistently takes into account the fact that the beam electrons lose the kinetic energy of their motion along the magnetic field almost without any collisions under the action of the reverse-current electric field. Some of the electrons return from the emission source to the acceleration region without losing the kinetic energy of their transverse motion. Based on the observed hard X-ray bremsstrahlung spectrum, the model allows the injection spectrum of accelerated electrons to be reconstructed with a high accuracy. As an example, we consider the white-light flare of December 6, 2006, which was observed with a high spatial resolution in the optical wavelength range at the main maximum of hard X-ray emission. Within the framework of our model, we show that to explain the hard X-ray spectrum, the flux density of the energy transferred by electrons with energies above 18 keV was ˜3 × 1013 erg cm-2 s-1. This exceeds the habitual values typical of the classical model of a thick target without a reverse current by two orders of magnitude. The electron density in the beam is also very high: ˜1011 cm-3. A more careful consideration of plasma processes in such dense electron beams is needed when the physical parameters of a flare are calculated.

  9. Modeling of Present-Day Atmosphere and Ocean Non-Tidal De-Aliasing Errors for Future Gravity Mission Simulations

    NASA Astrophysics Data System (ADS)

    Bergmann-Wolf, I.; Dobslaw, H.; Mayer-Gürr, T.

    2015-12-01

    A realistically perturbed synthetic de-aliasing model consistent with the updated Earth System Model of the European Space Agency (Dobslaw et al., 2015) is now available for the years 1995 -- 2006. The data-set contains realizations of (i) errors at large spatial scales assessed individually for periods between 10 -- 30, 3 -- 10, and 1 -- 3 days, the S1 atmospheric tide, and sub-diurnal periods; (ii) errors at small spatial scales typically not covered by global models of atmosphere and ocean variability; and (iii) errors due to physical processes not represented in currently available de-aliasing products. The error magnitudes for each of the different frequency bands are derived from a small ensemble of four atmospheric and oceanic models. In order to demonstrate the plausibility of the error magnitudes chosen, we perform a variance component estimation based on daily GRACE normal equations from the ITSG-Grace2014 global gravity field series recently published by the University of Graz. All 12 years of the error model are used to calculate empirical error variance-covariance matrices describing the systematic dependencies of the errors both in time and in space individually for five continental and four oceanic regions, and daily GRACE normal equations are subsequently employed to obtain pre-factors for each of those matrices. For the largest spatial scales up to d/o = 40 and periods longer than 24 h, errors prepared for the updated ESM are found to be largely consistent with noise of a similar stochastic character contained in present-day GRACE solutions. Differences and similarities identified for all of the nine regions considered will be discussed in detail during the presentation.Dobslaw, H., I. Bergmann-Wolf, R. Dill, E. Forootan, V. Klemann, J. Kusche, and I. Sasgen (2015), The updated ESA Earth System Model for future gravity mission simulation studies, J. Geod., doi:10.1007/s00190-014-0787-8.

  10. Exploitation or cooperation? Evolution of a host (ciliate)-benefiting alga in a long-term experimental microcosm culture.

    PubMed

    Nakajima, Toshiyuki; Matsubara, Toshiyuki; Ohta, Yuko; Miyake, Daisuke

    2013-09-01

    Controversy persists as to whether the acquisition of beneficial metabolic functions via endosymbiosis can occur suddenly on an evolutionary time scale. In this study, an early stage of endosymbiotic associations, which evolved from previously unassociated auto (photo)- and heterotrophic unicellular organisms was analyzed using an experimental ecosystem model, called CET microcosm. This ecosystem model was composed of a green alga (Micractinium sp.; formerly described as Chlorella vulgaris), a bacterium (Escherichia coli), and a ciliate (Tetrahymena thermophila). Our previous study using a CET microcosm that was cultured 3-5 years revealed that fitness of the ciliate increased by harboring algal cells within its own cells. This fact suggested three possibilities: (i) the ciliate evolved the ability to exploit intracellular algal cells ("exploiter ciliate hypothesis"), (ii) the alga evolved the ability to benefit the host ciliate by providing photosynthates ("cooperator alga hypothesis"), and (iii) a combination of (i) and (ii). To test these hypotheses, two-by-two co-cultures were conducted between the ancestral or derived ciliate and the ancestral or derived alga. The experimental results demonstrated that a cooperative alga evolved in the microcosm, although the possibility remains that an exploitative genotype of the ciliate might also exist in the population as a polymorphism. Remarkably, an algal isolate prolonged the longevity of not only the isolated ciliate, but also the ancestral ciliate. This result suggests that once a cooperative algal genotype evolves in a local population, it can then be transmitted to other individuals of the prospective host species and spread rapidly beyond the local range due to its positive effect on the host fitness. Such transmission suggests the possibility of a sudden acquisition of beneficial autotrophic function by the pre-associated host.

  11. Differential Expression of Tomato Spotted Wilt Virus-Derived Viral Small RNAs in Infected Commercial and Experimental Host Plants

    PubMed Central

    Mitter, Neena; Koundal, Vikas; Williams, Sarah; Pappu, Hanu

    2013-01-01

    Background Viral small RNAs (vsiRNAs) in the infected host can be generated from viral double-stranded RNA replicative intermediates, self-complementary regions of the viral genome or from the action of host RNA-dependent RNA polymerases on viral templates. The vsiRNA abundance and profile as well as the endogenous small RNA population can vary between different hosts infected by the same virus influencing viral pathogenicity and host response. There are no reports on the analysis of vsiRNAs of Tomato spotted wilt virus (TSWV), a segmented negative stranded RNA virus in the family Bunyaviridae, with two of its gene segments showing ambisense gene arrangement. The virus causes significant economic losses to numerous field and horticultural crops worldwide. Principal Findings Tomato spotted wilt virus (TSWV)-specific vsiRNAs were characterized by deep sequencing in virus-infected experimental host Nicotiana benthamiana and a commercial, susceptible host tomato. The total small (s) RNA reads in TSWV-infected tomato sample showed relatively equal distribution of 21, 22 and 24 nt, whereas N. benthamiana sample was dominated by 24 nt total sRNAs. The number of vsiRNA reads detected in tomato was many a magnitude (~350:1) higher than those found in N. benthamiana, however the profile of vsiRNAs in terms of relative abundance 21, 22 and 24 nt class size was similar in both the hosts. Maximum vsiRNA reads were obtained for the M RNA segment of TSWV while the largest L RNA segment had the least number of vsiRNAs in both tomato and N. benthamiana. Only the silencing suppressor, NSs, of TSWV recorded higher antisense vsiRNA with respect to the coding frame among all the genes of TSWV. Significance Details of the origin, distribution and abundance of TSWV vsiRNAs could be useful in designing efficient targets for exploiting RNA interference for virus resistance. It also has major implications toward our understanding of the differential processing of vsiRNAs in antiviral

  12. Mule deer (Odocoileus hemionus) and elk (Cervus elaphus) as experimental definitive hosts for Fascioloides magna.

    PubMed

    Foreyt, W J

    1996-10-01

    In August 1992, six mule deer (Odocoileus hemionus hemionus) fawns and four elk (Cervus elaphus) calves (n = 2) or yearlings (n = 2) each were inoculated orally with 50, 250, or 2,000 metacercariae of the liver fluke Fascioloides magna to evaluate their potential to serve as definitive hosts. Animals were maintained for up to 403 days. Three mule deer each inoculated with 50 metacercariae survived the infection and shed eggs in feces; thus mule deer can function as definitive hosts for F. magna. The other three mule deer inoculated with 50 (n = 1) or 250 (n = 2) metacercariae died from fluke infection on days 91, 150, and 162 days postinoculation, respectively, and only immature F. magna were recovered. One elk calf inoculated with 2,000 metacercariae died from fluke infection 44 days after inoculation. The remaining three elk, each inoculated with 250 metacercariae, survived infection, and two of the three shed eggs in feces. The third elk contained only one immature F. magna at necropsy. The prepatent period in mule deer and elk was approximately 6 to 7 months.

  13. Performance of regional climate models in simulations of present-day Irish climate: Implications for constructing future scenarios

    NASA Astrophysics Data System (ADS)

    Foley, A. M.

    2009-04-01

    Simulations of present-day (1961-1990) climate from 19 regional climate model experiments have been compared to the observed baseline climate for Ireland. These simulations, driven by global climate models, are obtained through the EC PRUDENCE (Prediction of Regional scenarios and Uncertainties for Defining EuropeaN Climate change risks and Effects) project. The ability to represent the statistics of Irish climate has been assessed, both temporally (comparisons of meteorological year, seasonal mean and seasonal variance) and spatially (seasonal covariation and pattern-analysis) for two key meteorological parameters, that of temperature and precipitation. For the average meteorological year (30-year averages of each month), mean temperatures are found to be within 1.5˚ C of observations, except in winter, when temperatures are overestimated by up to 2.5˚ C. Temporal variation is also not well-represented by some models in winter. Conversely, temporal variation in precipitation is most poorly simulated in summer. Seasonal variance is the area in which greatest inter-model variability is shown. Ratio of observed variance to modeled variance ranges from weak (0.5 or less) to very strong (greater than 0.7) in both seasons, and for both parameters. Spatially, temperature is over-estimated throughout Ireland, by up to 4.6˚ C in winter and 2.7˚ C in summer in individual grid cells from some models. Precipitation is found to be both under and over-estimated, with grid cell biases ranging from -2.5 mm/day to 4.2 mm/day in winter and from -1.2 mm/day to 1.8 mm/day in summer. While skill at representing the spatial precipitation pattern is found to be very strong in 16 out of 19 experiments in winter, only 2 of those experiments show the same level of skill in summer. Errors are identified in all individual models, both systematic and random. While using an ensemble average overcomes some of these deficiencies, the optimal approach is to correct systematic errors

  14. Investigating the Present Day Cosmic Dust Flux at the Earth's Surface: Initial Results from the Kwajalein Micrometeorite Collection

    NASA Technical Reports Server (NTRS)

    Wozniakiewicz, P. J.; Bradley, J. P.; Price, M. C.; Zolensky, M. E.; Ishii, H. A.; Brownlee, D. E.; Russell, S. S.

    2014-01-01

    Examination of impact craters on the Long Duration Exposure Facility satellite indicate a present day micrometeoroid flux of approx. 30,000 tonnes [1 after 2]. But what portion of this material arrives at the Earth's surface as micrometeorites? Studies of available micrometeorite collections from deep sea sediments [e.g. 3], Greenland blue ice [e.g. 4] and the South Pole water well [e.g. 1] may be complicated by terrestrial weathering and, in some cases, collection bias (magnetic separation for deep sea sediments) and poorly constrained ages. We have recently set up a micrometeorite collection station on Kwajalein Island in the Republic of the Marshall Islands in the Pacific Ocean, using high volume air samplers to collect particles directly from the atmosphere. By collecting in this way, the terrestrial age of the particles is known, the weathering they experience is minimal, and we are able to constrain particle arrival times. Collecting at this location also exploits the considerably reduced anthropogenic background [5]. Method: High volume air samplers were installed on top of the two-story airport building on Kwajalein. These were fitted with polycarbonate membrane filters with 5µm diameter perforations. The flow rates were set to 0.5m3/min, and filters were changed once a week. After collection, filters were washed to remove salt and concentrate particles [see 5] in preparation for analysis by SEM. Results and Discussion: A selection of filters have been prepared and surveyed. Due to their ease of identification our initial investigations have focused on particles resembling cosmic spherules. The spheres can be divided into three main groups: 1. Silicate spherules rich in Al, Ca, K and Na (to varying degrees), 2. Silicate spherules rich in Mg and Fe and 3. Fe-rich spherules. Group 1 spherules are often vesiculated and can occur as aggregates. They are similar in appearance and composition to volcanic microspheres [e.g. 6] and are thus likely terrestrial in

  15. Mantle-circulation models with sequential data assimilation: inferring present-day mantle structure from plate-motion histories.

    PubMed

    Bunge, Hans-Peter; Richards, M A; Baumgardner, J R

    2002-11-15

    limited in age to the Cretaceous. This result implies that sequential assimilation of past plate-motion models is ineffective in studying the temporal evolution of core-mantle-boundary heterogeneity, and that a method for extrapolating present-day information backwards in time is required. For short time periods (of the order of perhaps a few tens of Myr) such a method exists in the form of crude 'backward' convection calculations. For longer time periods (of the order of a mantle overturn), a rigorous approach to extrapolating information back in time exists in the form of iterative nonlinear optimization methods that carry assimilated information into the past through the use of an adjoint mantle convection model. PMID:12460480

  16. Mantle-circulation models with sequential data assimilation: inferring present-day mantle structure from plate-motion histories.

    PubMed

    Bunge, Hans-Peter; Richards, M A; Baumgardner, J R

    2002-11-15

    limited in age to the Cretaceous. This result implies that sequential assimilation of past plate-motion models is ineffective in studying the temporal evolution of core-mantle-boundary heterogeneity, and that a method for extrapolating present-day information backwards in time is required. For short time periods (of the order of perhaps a few tens of Myr) such a method exists in the form of crude 'backward' convection calculations. For longer time periods (of the order of a mantle overturn), a rigorous approach to extrapolating information back in time exists in the form of iterative nonlinear optimization methods that carry assimilated information into the past through the use of an adjoint mantle convection model.

  17. Present-Day Kinematics of Crustal Deformation of the Walker Lane and Central Nevada, Derived From GPS Data

    NASA Astrophysics Data System (ADS)

    Nyst, M. C.; Hammond, W. C.

    2002-12-01

    The Walker Lane (WL) is a zone of tectonic deformation located between the western edge of the Basin and Range province (BR) in Nevada and the Sierra Nevada in California. It extends from ~36o in California to ~41o latitude in western Nevada and eastern California. The WL is of great interest for the study of intra-plate deformation because it accommodates the transfer of NW directed motion of the Sierra Nevada block to roughly E-W extension in the eastern BR. In addition, by connecting the Eastern California Shear Zone (ECSZ) with more northern zones of dextral shear such as the Central Nevada Seismic Zone (CNSZ) the WL plays an important role in the accommodation of motion between the Pacific and North America plates. Previous studies of the present-day crustal deformation in this area focused on sub-regions of the WL. We study the whole WL and utilize a relatively dense GPS velocity set (of 121 stations with data from 1992 to 2002) to model crustal deformation. The GPS data set consists of the western Nevada data of Svarc et al. (JGR, 2002), the BR data of Thatcher et al. (Science, 1999) and the ECSZ data of Gan et al. (JGR, 2000). Principal strain rates derived from data subsets agree with those of previous studies: In the northern WL WNW-ESE extension averages ~24 nstrain/yr and NNE-SSW contraction averages ~9 nstrain/yr. Since the average fault trend is NNW, the resulting strain rates may be indicative for dextral transtensional slip. In the central WL WNW-ESE extension (~27 nstrain/yr) and NNE-SSW contraction (~11 nstrain/yr) together with the predominantly ENE trending faults suggest sinistral transtensional slip. In the southern WL ENE-WSW extension (~25 nstrain/yr), NNW-SSE contraction (~30 nstrain/yr) and NNW trending faults correspond to dextral slip. To determine the spatial variation of the crustal deformation field we solve for a self-consistent continuous velocity gradient field from which both strain and rotation rate fields are derived. In general

  18. Impact of urban emission on air-quality over central Europe: present day and future emissions perspective

    NASA Astrophysics Data System (ADS)

    Huszar, Peter; Belda, Michal; Halenka, Tomas; Karlicky, Jan

    2016-04-01

    The purpose of the study is to quantify the impact of present-day and future urban emission from central European cities on the regional air-quality (AQ), based on a modeling couple of the regional climate model RegCM4.2 and the chemistry transport model CAMx, including two-way interactions. A series of simulations was carried out for the present (2001-2010) decade and two future decades (2026-2035 and 2046-2055) either with all urban emissions included (base case) or without considering urban emissions. As we are interested on the impact of emission changes only, the impact of different driving meteorological conditions in the future (due to climate change) are not considered. The emissions used is the TNO MEGAPOLI European emission database that includes country/sector based scenarios for years 2030 and 2050, which were used for the encompassing decades. Further, the sensitivity of ozone production to urban emissions was examined by performing reduction experiments with -20% emission perturbation of NOx and/or NMVOC. The model was also validated using surface measurements of key pollutants. Selected air-quality measures were used as metrics describing the cities emission impact on regional air pollution. Due to urban emissions, significant ozone titration occurs over cities while over rural areas further from, ozone production is modeled, mainly in terms of number of exceedances and accumulated exceedances over the threshold of 40 ppbv. Urban NOx, SO2 and PM2.5 emissions also significantly contribute to concentrations in the cities themselves (up to 50-70% for NOx and SO2 , and up to 55% for PM2.5), but the contribution is large over rural areas as well (10-20%). Although air pollution over cities is largely determined by the local urban emissions, considerable (often a few tens of %) fraction of the concentration is attributable to other sources from rural areas and minor cities. The future urban emission AQ fingerprint is, in general, slightly smaller than in

  19. Triggering Receptor Expressed on Myeloid Cells (TREM)-2 Impairs Host Defense in Experimental Melioidosis

    PubMed Central

    Weehuizen, Tassili A. F.; Hommes, Tijmen J.; Lankelma, Jacqueline M.; de Jong, Hanna K.; Roelofs, Joris. J.T.H.; de Vos, Alex F.; Colonna, Marco; van der Poll, Tom; Wiersinga, W. Joost

    2016-01-01

    Background Triggering receptor expressed on myeloid cells (TREM) -1 and TREM-2 are key regulators of the inflammatory response that are involved in the clearance of invading pathogens. Melioidosis, caused by the "Tier 1" biothreat agent Burkholderia pseudomallei, is a common form of community-acquired sepsis in Southeast-Asia. TREM-1 has been suggested as a biomarker for sepsis and melioidosis. We aimed to characterize the expression and function of TREM-1 and TREM-2 in melioidosis. Methodology/Principal Findings Wild-type, TREM-1/3 (Trem-1/3-/-) and TREM-2 (Trem-2-/-) deficient mice were intranasally infected with live B. pseudomallei and killed after 24, and/or 72 h for the harvesting of lungs, liver, spleen, and blood. Additionally, survival studies were performed. Cellular functions were further analyzed by stimulation and/or infection of isolated cells. TREM-1 and TREM-2 expression was increased both in the lung and liver of B. pseudomallei-infected mice. Strikingly, Trem-2-/-, but not Trem-1/3-/-, mice displayed a markedly improved host defense as reflected by a strong survival advantage together with decreased bacterial loads, less inflammation and reduced organ injury. Cellular responsiveness of TREM-2, but not TREM-1, deficient blood and bone-marrow derived macrophages (BMDM) was diminished upon exposure to B. pseudomallei. Phagocytosis and intracellular killing of B. pseudomallei by BMDM and alveolar macrophages were TREM-1 and TREM-2-independent. Conclusions/Significance We found that TREM-2, and to a lesser extent TREM-1, plays a remarkable detrimental role in the host defense against a clinically relevant Gram-negative pathogen in mice: TREM-2 deficiency restricts the inflammatory response, thereby decreasing organ damage and mortality. PMID:27253382

  20. Accumulation and dissemination of prion protein in experimental sheep scrapie in the natural host

    PubMed Central

    Ryder, Stephen J; Dexter, Glenda E; Heasman, Lindsay; Warner, Richard; Moore, S Jo

    2009-01-01

    Background In order to study the sites of uptake and mechanisms of dissemination of scrapie prions in the natural host under controlled conditions, lambs aged 14 days and homozygous for the VRQ allele of the PrP gene were infected by the oral route. Infection occurred in all lambs with a remarkably short and highly consistent incubation period of approximately 6 months. Challenge of lambs at approximately eight months of age resulted in disease in all animals, but with more variable incubation periods averaging significantly longer than those challenged at 14 days. This model provides an excellent system in which to study the disease in the natural host by virtue of the relatively short incubation period and close resemblance to natural infection. Results Multiple sites of prion uptake were identified, of which the most important was the Peyer's patch of the distal ileum. Neuroinvasion was detected initially in the enteric nervous system prior to infection of the central nervous system. At end stage disease prion accumulation was widespread throughout the entire neuraxis, but vacuolar pathology was absent in most animals that developed disease at 6–7 months of age. Conclusion Initial spread of detectable PrP was consistent with drainage in afferent lymph to dependent lymph nodes. Subsequent accumulation of prions in lymphoid tissue not associated with the gut is consistent with haematogenous spread. In addition to macrophages and follicular dendritic cells, prion containing cells consistent with afferent lymph dendritic cells were identified and are suggested as a likely vehicle for carriage of prions from initial site of uptake to the lymphoreticular system, and as potential carriers of prion protein in blood. It is apparent that spongiform change, the characteristic lesion of scrapie and other prion diseases, is not responsible for the clinical signs in sheep, but may develop in an age dependent manner. PMID:19243608

  1. PRESENT-DAY MASS FUNCTION OF SIX SMALL MAGELLANIC CLOUD INTERMEDIATE-AGE AND OLD STAR CLUSTERS

    SciTech Connect

    Glatt, Katharina; Grebel, Eva K.; Jordi, Katrin; Gallagher, John S. III; Harbeck, Daniel; Da Costa, Gary; Clementini, Gisella; Tosi, Monica; Nota, Antonella; Sabbi, Elena; Sirianni, Marco

    2011-08-15

    We determined the present-day mass functions (PDMFs) of the five intermediate-age star clusters Lindsay 1, Kron 3, NGC 339, NGC 416, and Lindsay 38 and the old star cluster NGC 121 in the Small Magellanic Cloud (SMC) based on observations with the Hubble Space Telescope Advanced Camera for Surveys. The global PDMFs are well matched by Salpeter-like power laws from their main-sequence turnoffs to {approx}0.6 M{sub sun} with a power-law exponent {alpha} ranging from 1.51 {+-} 0.11 (Lindsay 1) to 2.29 {+-} 0.15 (NGC 339). We derive total stellar masses of {approx}10{sup 5} M{sub sun}, except for Lindsay 38, whose mass is of the order of {approx}10{sup 4} M{sub sun}. Differences between the PDMFs most likely reflect the varying stages of dynamical evolution of the clusters. These SMC clusters do not follow the {alpha} versus concentration parameter c correlation as found for Galactic globular clusters of similar mass. This might be an age effect or due to their location in a galaxy where bulge and disk crossings do not play a role. No correlation is found between {alpha} and the cluster core and tidal radii (r{sub c} and r{sub t} , respectively), the half-light radii r{sub h} , age, central surface brightness, metallicity, and galactocentric radius r{sub gc}. All six clusters mass-segregated to different degrees. The two clusters Lindsay 1 and Kron 3 barely show signs for mass segregation, but have low-mass star deficient global PDMFs and might be the remnants of star clusters whose outer parts were stripped. A trend exists between the degree of mass segregation and the ratio age/relaxation time t{sub r,h}, which indicates the stage of dynamical evolution for a cluster. Our data thus suggest that the SMC clusters in the present sample had a range of initial densities and presumably different amounts of mass loss that led to different rates of dynamical evolution. The clusters' positions in the r{sub h,m}/r{sub t} versus r{sub 0}/r{sub h,m} plane imply that all of the

  2. Targeting host syntaxin-5 preferentially blocks Leishmania parasitophorous vacuole development in infected cells and limits experimental Leishmania infections.

    PubMed

    Canton, Johnathan; Kima, Peter E

    2012-10-01

    Our previous observations established a role for syntaxin-5 in the development of Leishmania parasitophorous vacuoles (LPVs). In this study, we took advantage of the recent identification of Retro-2, a small organic molecule that can cause the redistribution of syntaxin-5; we show herein that Retro-2 blocks LPV development within 2 hours of adding it to cells infected with Leishmania amazonensis. In infected cells incubated for 48 hours with Retro-2, LPV development was significantly limited; furthermore, infected cells harbored four to five times fewer parasites than infected cells incubated in vehicle alone. In vivo studies revealed that Retro-2 curbed experimental L. amazonensis infections in a dose-dependent manner. Retro-2 did not have any appreciable effect on the host cell physiological characteristics; furthermore, it had no apparent toxicity in experimental animals. An unexpected, but welcome, finding was that Retro-2 inhibited the replication of Leishmania parasites in axenic cultures. This study is significant because it identifies an endoplasmic reticulum/Golgi SNARE as a potential target for the control of Leishmania infections; moreover, it suggests that small organic molecules can be identified that can selectively disrupt the vesicle fusion machinery that promotes the development of pathogen-containing compartments without exerting toxic effects on the host.

  3. Effect of sublethal gamma radiation on host defenses in experimental scrub typhus

    SciTech Connect

    Kelly, D.J.; Rees, J.C.

    1986-06-01

    The effect of sublethal gamma radiation on inbred mice chronically infected with scrub typhus rickettsiae was examined. Inbred mice which were inoculated with the Gilliam or Karp strain of Rickettsia tsutsugamushi by the subcutaneous route harbored the infection for at least 1 year. Irradiation of these animals at 12 or 52 weeks postinoculation with normally sublethal levels induced a significantly higher percentage of rickettsemic mice (recrudescence) than was seen in the unirradiated, similarly infected control animals. In addition, sublethal irradiation at 12 weeks induced a quantitative increase in total rickettsiae. Homologous antibody titers to the rickettsiae were examined for 5 weeks after irradiation to determine the role of the humoral response in radiation-induced recrudescence. Unirradiated, infected mice showed consistent titers of about 320 throughout the 5-week observation period, and the titer was not affected by exposure of up to 500 rads of gamma radiation. Drug dose-dependent radioprotection and modification of recrudescence was noted in infected, irradiated mice treated with the antiradiation compound S-2-(3-aminopropylamino)ethyl phosphorothioic acid. The results of this investigation supported the conclusion that the recrudescence of a chronic rickettsial infection in the appropriate host after immunological impairment due to gamma radiation can result in an acute, possibly lethal rickettsemia.

  4. Host defenses in experimental scrub typhus: effect of sublethal gamma radiation

    SciTech Connect

    Kelly, D.J.

    1983-01-01

    The effect of sublethal gamma radiation on inbred mice chronically infected with scrub typhus rickettsiae was examined. Inbred mice which have been inoculated with Gilliam or Karp strain of Rickettsia tsutsugamushi by the subcutaneous route harbored the infection for at least one year. Irradiation of these animals at 12 or 52 weeks post inoculation at normally sublethal levels induced a significantly higher percentage of rickettsemic mice (recrudescence) than in the unirradiated similarly infected control animals. In addition, sublethal irradiation at 12 weeks also induced a quantitative increase in total rickettsiae. Homologous antibody titers to the rickettsiae were examined for five weeks following irradiation to determine the role of the humoral response in radiation induced recrudescence. Modification of recrudescence was investigated using radioprotective drugs. The expected results of this investigation supported the conclusion that the recrudescence of a chronic rickettsial infection in the appropriate host following immunological impairment due to battlefield or clinical exposure to gamma radiation can result in an acute, possibly lethal rickettsemia.

  5. The effect of glucan--a host resistance activator--and ampicillin on experimental intraabdominal sepsis.

    PubMed

    Lahnborg, G; Hedström, K G; Nord, C E

    1982-11-01

    Glucan, a beta-1-3-polyglucosidic component of the cell wall of Saccharomyces cervisiae, was evaluated for its ability to influence the survival rate in rats with induced intraabdominal sepsis. To mimic closely the human bacteriological intestinal flora, the rats, in 4 groups each of 15 animals, were fed a lean meat diet. Intraabdominal sepsis was induced by resecting 1 cm of the intestine and reimplanting it in the abdominal cavity, reestablishing intestinal continuity by one-layer end-to-end anastomosis. The rats were injected with glucan, isovolumetric saline, and ampicillin or glucan plus ampicillin. The results indicate a significant decrease in mortality in the group treated with ampicillin compared with the group treated with saline only. The group treated with glucan plus ampicillin differed significantly from the group given ampicillin. The bacterial flora was not qualitatively influenced by glucan administration. It is concluded that glucan, in combination with ampicillin, has a significant effect on the survival rate of rats with induced peritonitis, probably by enhancing the activities of the reticuloendothelial system--an important part of the total host resistance. PMID:7161767

  6. [Impact of present-day forms of organization of physical education on the health status of preschool children].

    PubMed

    Kuchma, V R; Vishnevskaia, T Iu; Makarova, A Iu

    2006-01-01

    During a natural hygienic experiment, the physical development, psychomotor activity, exercise performance, and readiness were evaluated in 6-year-old children, in whom physical education had been organized by routine and experimental programs, including health-improving swimming in the indoor pool of a preschool educational establishment. Exercises built up on the principle of plot-role playing games, by using the developing corrective exercises and psychological and pedagogical escort, were established to be highly effective. The proposed methods contribute to the timely harmonious development of a child, his movement characteristics, positively affect the neurofunctional status, by ensuring the optimum psychomotor development, and maintain a high exercise performance.

  7. The environment-pathogen-host axis in communicable and non-communicable diseases: recent advances in experimental and clinical research.

    PubMed

    Gilles, Stefanie; Traidl-Hoffmann, Claudia

    2014-05-01

    Allergies and autoimmune diseases are spreading worldwide. Control of infections, on the other hand, remains an issue, even in the post-antibiotic era. Chronic or poorly controlled infections occur in immune compromised individuals such as HIV patients, hospitalized patients exposed to multi-resistant bacteria, or patients on immunosuppressive treatment. They may become an even more emerging issue in an ageing population. At the same time, profound environmental changes such as global warming, urbanization, increasing environmental pollution and novel food engineering technologies may alter the abundance or aggressiveness of allergens/allergen carriers in our environment. Likewise, changes in dietary habits - and possibly also use of antibiotics - have an impact on the composition of our natural microbial flora in the gut, airways and skin, which may alter susceptibility for common diseases, among them allergies, asthma and atopic eczema. At the recently founded Institute of Environmental Medicine of the Technische Universität Munich, located in Augsburg at the UNIKA-T, experimental, clinical and translational research is focused on the complex interactions of environment, pathogen and host in expression or control of communicable and non-communicable diseases. We present our research concept and recent findings in environment - host interactions.

  8. Experimental shifts in egg-nest contrasts do not alter egg rejection responses in an avian host-brood parasite system.

    PubMed

    Hauber, Mark E; Aidala, Zachary; Igic, Branislav; Shawkey, Matthew D; Moskát, Csaba

    2015-09-01

    Obligate brood parasitic birds exploit their hosts to provide care for unrelated young in the nest. Potential hosts can reduce the cost of parasitism by rejecting foreign eggs from the nest. Observational, comparative, and experimental studies have concluded that most hosts use the coloration and patterning of eggshells to discriminate between own and foreign eggs in the nest. However, an alternative hypothesis is that birds use the colour contrasts between eggshells and the nest lining to identify parasitic eggs (egg-nest contrast hypothesis). In support of this hypothesis, we found that the avian perceivable chromatic contrasts between dyed eggs and unmanipulated nest linings significantly and negatively covaried with the rejection rates of different dyed eggs of the great reed warbler Acrocephalus arundinaceus, a frequently parasitized host of the common cuckoo Cuculus canorus. To experimentally test whether egg-nest contrasts influence rejection, we reciprocally dyed both eggs and the nest lining of this host species with one of two colours: orange and green. Contrary to the egg-nest contrast hypothesis, host rejection patterns in response to dyed eggs were not altered by dyeing nests, relative to unmanipulated control eggs and nests. In turn, experimental egg colour was the only significant predictor of egg rejection rate. Our results demonstrate that egg-nest contrast is a collateral, not a causal factor in egg rejection, and confirm the conclusions of previous studies that hosts can rely on the parasitic egg's appearance itself to recognize the foreign egg in the nest. PMID:26118673

  9. REE and Hf distribution among mineral phases in the CV-CK clan: A way to explain present-day Hf isotopic variations in chondrites

    NASA Astrophysics Data System (ADS)

    Martin, Céline; Debaille, Vinciane; Lanari, Pierre; Goderis, Steven; Vandendael, Isabelle; Vanhaecke, Frank; Vidal, Olivier; Claeys, Philippe

    2013-11-01

    as the degree of metamorphism increases (30% for types 3 and 4, less than 5% in type 6). In contrast to Lu, Hf is mainly hosted by silicates with little contribution from phosphates throughout the CK metamorphic sequence. A significant part of Sm and Nd are stored in phosphates in types 3-5, and these elements behave similarly during CK chondrite metamorphism. That explains the robustness of the Sm/Nd ratios in chondrites through metamorphism, and the slight discrepancies observed in the present-day isotopic Nd values in chondrites. On the contrary, Lu and Hf are borne by several different minerals and consequently they are redistributed during metamorphism-induced recrystallization. The Lu/Hf ratios are therefore significantly disturbed during chondrites metamorphism, leading to the high discrepancies observed in present-day Hf isotopic values in chondrites.

  10. The role of Staphylococcal carotenogenesis in resistance to host defense peptides and in vivo virulence in experimental endocarditis model.

    PubMed

    Xiong, Yan Q; Yang, Soo-Jin; Tong, Steven Y C; Alvarez, Danya N; Mishra, Nagendra N

    2015-10-01

    The defining hallmark of the newly described species, Staphylococcus argenteus, in comparison to its sister species, S. aureus and S. schweitzeri, is the absence of production of the carotenoid pigment, staphyloxanthin. Staphylococcus argenteus lacks the responsible genetic locus crtOPQMN. We examined the impact of carotenoid synthesis in two non-pigmented S. argenteus strains, MSHR1132 and SCC1165. Following complementation with a plasmid containing the carotenoid operon (pTX-crtOPQMN), compared to wild type, both complemented strains showed substantial carotenoid production, with a resultant increase in cell membrane rigidity. Surprisingly, both crtOPQMN-complemented strains exhibited increased susceptibility to the host defense peptides, LL-37 and hNP-1 in vitro, and reduced virulence in an experimental rabbit endocarditis model.

  11. Vertebrate host specificity and experimental vectors of Plasmodium (Novyella) kempi sp. n. from the eastern wild turkey in Iowa.

    PubMed

    Christensen, B M; Barnes, H J; Rowley, W A

    1983-07-01

    Vertebrate host specificity, experimental laboratory vectors, and a description of Plasmodium (Novyella) kempi sp. n. from eastern wild turkeys (Meleagris gallopavo silvestris Vieillot) in Iowa are presented. Plasmodium kempi is infective for domestic turkeys, bobwhites (Colinus virginianus), chukars (Alectoris graeca), guinea fowl (Numida meleagris), peacocks (Pavo cristatus), and canaries (Serinus canaria), produces a transient infection in mallards (Anas platyrhynchos) and domestic geese (Anser anser), but will not infect ring-necked pheasants (Phasianus colchicus), pigeons (Columba livia), Japanese quail (Coturnix coturnix), leghorn white chickens (Gallus gallus), or starlings (Sturnus vulgaris). Oocysts and (or) sporozoites were recovered from 68% (84/124) and 98% (60/61) of the Culex pipiens pipiens and C. tarsalis examined, respectively. Oocysts developed faster and sporozoites invaded the salivary glands sooner in C. tarsalis (6 days) than in C. p. pipiens (7 days). Culex tarsalis transmitted P. kempi more effectively than C. p. pipiens, although both species were capable of transmitting the parasite by natural feeding. Oocysts developed and sporozoites also were produced in C. restuans, but its ability to transmit the parasite was not determined. Aedes aegypti (Rockefeller strain) and A. triseriatus were refractive to P. kempi. Plasmodium kempi produces trophozoites with large refractile globules and fine cytoplasmic extensions, mature schizonts in the form of a condensed fan containing four to eight nuclei (usually 5), and elongate gametocytes with irregular borders. All stages are confined almost exclusively to mature erythrocytes, with no effect on host cell size or position of host cell nucleus. Plasmodium kempi is most similar morphologically to P. (Novyella) hexamerium and P. (Novyella) vaughani. It differs from P. hexamerium in having large refractile globules in trophozoites and immature schizonts, an inability to infect starlings, an absence of

  12. Description of advanced third-stage larvae of Gnathostoma lamothei Bertoni-Ruiz et al. 2005 (Nematoda: Gnathostomatidae) from experimental hosts and contributions to its life cycle.

    PubMed

    Gaspar-Navarro, Jorge; Almeyda-Artigas, Roberto Javier; Sánchez-Miranda, Elizabeth; Carranza-Calderón, Laura; Mosqueda-Cabrera, Miguel Angel

    2013-01-01

    The advanced third-stage larvae (AdvL(3)) of Gnathostoma lamothei was obtained from experimental hosts. Frogs Lithobates heckscheri and snakes Nerodia fasciata pictiventris were compatible hosts allowing optimal larval development. AdvL(3) are 4,487.94 μm long, have two lateral cervical papillae between rows 10 and 16 and an excretory pore at row 23. The average counts of the cephalic bulb hooklets from the four rows are 39.3, 43.3, 44.2, and 47.3. Larvae show an esophagus that represents 40 % of the body width. These findings indicate that amphibians and reptiles could be involved as G. lamothei natural hosts; nevertheless, their role as etiological agents of human gnathostomiasis is uncertain. This paper reports for the first time the taxonomic description of G. lamothei AdvL(3) obtained from experimental hosts and contributes to the understanding of its life cycle.

  13. Experimental shifts in intraclutch egg color variation do not affect egg rejection in a host of a non-egg-mimetic avian brood parasite.

    PubMed

    Croston, Rebecca; Hauber, Mark E

    2015-01-01

    Avian brood parasites lay their eggs in the nests of other birds, and impose the costs associated with rearing parasitic young onto these hosts. Many hosts of brood parasites defend against parasitism by removing foreign eggs from the nest. In systems where parasitic eggs mimic host eggs in coloration and patterning, extensive intraclutch variation in egg appearances may impair the host's ability to recognize and reject parasitic eggs, but experimental investigation of this effect has produced conflicting results. The cognitive mechanism by which hosts recognize parasitic eggs may vary across brood parasite hosts, and this may explain variation in experimental outcome across studies investigating egg rejection in hosts of egg-mimicking brood parasites. In contrast, for hosts of non-egg-mimetic parasites, intraclutch egg color variation is not predicted to co-vary with foreign egg rejection, irrespective of cognitive mechanism. Here we tested for effects of intraclutch egg color variation in a host of nonmimetic brood parasite by manipulating egg color in American robins (Turdus migratorius), hosts of brown-headed cowbirds (Molothrus ater). We recorded robins' behavioral responses to simulated cowbird parasitism in nests where color variation was artificially enhanced or reduced. We also quantified egg color variation within and between unmanipulated robin clutches as perceived by robins themselves using spectrophotometric measures and avian visual modeling. In unmanipulated nests, egg color varied more between than within robin clutches. As predicted, however, manipulation of color variation did not affect rejection rates. Overall, our results best support the scenario wherein egg rejection is the outcome of selective pressure by a nonmimetic brood parasite, because robins are efficient rejecters of foreign eggs, irrespective of the color variation within their own clutch.

  14. Phosphine oxide derivatives as hosts for blue phosphors: A joint theoretical and experimental study of their electronic structure

    SciTech Connect

    Kim, Dongwook; Salman, Seyhan; Coropceanu, Veaceslav; Salomon, Eric; Padmaperuma, Asanga B.; Sapochak, Linda S.; Kahn, Antoine; Bredas, Jean-Luc

    2010-01-12

    We report on a joint theoretical and experimental investigation of the electronic structure of a series of bis(diphenylphosphine oxide) derivatives containing a central aromatic core with high triplet energy. Such molecules can serve as host material in the emissive layer of blue electro-phosphorescent organic devices. The aromatic cores considered in the theoretical study consist of biphenyl, fluorene, dibenzofuran, dibenzothiophene, dibenzothiophenesulfone or carbazole, linked to the two phosphoryl groups in either para or meta positions. With respect to the isolated core molecules, it is found that addition of the diphenylphosphine oxide moieties has hardly any impact on the core geometry and only slightly reduces the energy of the lowest triplet state (by at most ~0.2 eV). However, the diphenylphosphine oxide functionalities significantly impact the ionization potential and electron affinity values, in a way that is different for para and meta substitutions. Excellent comparison is obtained between the experimental UPS and IPES spectra of the para biphenyl and meta dibenzothiophene and dibenzothiophenesulfone compounds and the simulated spectra. In general, the phosphine oxide derivatives present triplet energies that are calculated to be at least 0.2 eV higher than those of currently widely used blue phosphorescent emitters.

  15. Habitat heterogeneity drives the host-diversity-begets-parasite-diversity relationship: evidence from experimental and field studies.

    PubMed

    Johnson, Pieter T J; Wood, Chelsea L; Joseph, Maxwell B; Preston, Daniel L; Haas, Sarah E; Springer, Yuri P

    2016-07-01

    Despite a century of research into the factors that generate and maintain biodiversity, we know remarkably little about the drivers of parasite diversity. To identify the mechanisms governing parasite diversity, we combined surveys of 8100 amphibian hosts with an outdoor experiment that tested theory developed for free-living species. Our analyses revealed that parasite diversity increased consistently with host diversity due to habitat (i.e. host) heterogeneity, with secondary contributions from parasite colonisation and host abundance. Results of the experiment, in which host diversity was manipulated while parasite colonisation and host abundance were fixed, further reinforced this conclusion. Finally, the coefficient of host diversity on parasite diversity increased with spatial grain, which was driven by differences in their species-area curves: while host richness quickly saturated, parasite richness continued to increase with neighbourhood size. These results offer mechanistic insights into drivers of parasite diversity and provide a hierarchical framework for multi-scale disease research.

  16. Historic, pre-European settlement, and present-day contribution of wild ruminants to enteric methane emissions in the United States.

    PubMed

    Hristov, A N

    2012-04-01

    The objectives of this analysis were to estimate historic (pre-European settlement) enteric CH(4) emissions from wild ruminants in the contiguous United States and compare these with present-day CH(4) emissions from farmed ruminants. The analysis included bison, elk (wapiti), and deer (white-tailed and mule). Wild ruminants such as moose, antelope (pronghorn), caribou, and mountain sheep and goat were not included in the analysis because their natural range is mostly outside the contiguous United States or because they have relatively small population sizes. Data for presettlement and present-day population sizes, animal BW, feed intake, and CH(4) emission factors were adopted from various sources. Present-day CH(4) emissions from livestock were from recent United States Environmental Protection Agency estimates. The most important factor determining CH(4) emissions from wild ruminants in the presettlement period was the size of the bison population. Overall, enteric CH(4) emissions from bison, elk, and deer in the presettlement period were about 86% (assuming bison population size of 50 million) of the current CH(4) emissions from farmed ruminants in the United States. Present-day CH(4) emissions from wild ruminants (bison, elk, and deer) were estimated at 0.28 Tg/yr, or 4.3% of the emissions from domestic ruminants. Due to its population size (estimated at 25 million), the white-tailed deer is the most significant present-day wild ruminant contributor to enteric CH(4) emissions in the contiguous United States. PMID:22178852

  17. Historic, pre-European settlement, and present-day contribution of wild ruminants to enteric methane emissions in the United States.

    PubMed

    Hristov, A N

    2012-04-01

    The objectives of this analysis were to estimate historic (pre-European settlement) enteric CH(4) emissions from wild ruminants in the contiguous United States and compare these with present-day CH(4) emissions from farmed ruminants. The analysis included bison, elk (wapiti), and deer (white-tailed and mule). Wild ruminants such as moose, antelope (pronghorn), caribou, and mountain sheep and goat were not included in the analysis because their natural range is mostly outside the contiguous United States or because they have relatively small population sizes. Data for presettlement and present-day population sizes, animal BW, feed intake, and CH(4) emission factors were adopted from various sources. Present-day CH(4) emissions from livestock were from recent United States Environmental Protection Agency estimates. The most important factor determining CH(4) emissions from wild ruminants in the presettlement period was the size of the bison population. Overall, enteric CH(4) emissions from bison, elk, and deer in the presettlement period were about 86% (assuming bison population size of 50 million) of the current CH(4) emissions from farmed ruminants in the United States. Present-day CH(4) emissions from wild ruminants (bison, elk, and deer) were estimated at 0.28 Tg/yr, or 4.3% of the emissions from domestic ruminants. Due to its population size (estimated at 25 million), the white-tailed deer is the most significant present-day wild ruminant contributor to enteric CH(4) emissions in the contiguous United States.

  18. Now you see it, now you don't: flushing hosts prior to experimentation can predict their responses to brood parasitism.

    PubMed

    Hanley, Daniel; Samaš, Peter; Heryán, Josef; Hauber, Mark E; Grim, Tomáš

    2015-03-12

    Brood parasitic birds lay their eggs in other birds' nests, leaving hosts to raise their offspring. To understand parasite-host coevolutionary arms races, many studies have examined host responses to experimentally introduced eggs. However, attending parents often need to be flushed from their nests to add experimental eggs. If these birds witness parasitism events, they may recognize and reject foreign eggs more readily than parents who did not. We found that, after being flushed, female blackbirds, Turdus merula, remained close to their nests. Flushed females were more likely to eject foreign eggs and did so more quickly than females that were not flushed during experimentation. In contrast, flushing did not predict responses and latency to responses to parasitism by song thrush, Turdus philomelos, which flew farther from their nests and likely did not witness experimental parasitism. When statistically considering flushing, previously published conclusions regarding both species' response to experimental parasitism did not change. Nevertheless, we recommend that researchers record and statistically control for whether hosts were flushed prior to experimental parasitism. Our results have broad implications because more vigilant and/or bolder parents can gain more information about parasitism events and therefore have better chances of successfully defending against brood parasitism.

  19. Now you see it, now you don't: flushing hosts prior to experimentation can predict their responses to brood parasitism

    PubMed Central

    Hanley, Daniel; Samaš, Peter; Heryán, Josef; Hauber, Mark E.; Grim, Tomáš

    2015-01-01

    Brood parasitic birds lay their eggs in other birds' nests, leaving hosts to raise their offspring. To understand parasite-host coevolutionary arms races, many studies have examined host responses to experimentally introduced eggs. However, attending parents often need to be flushed from their nests to add experimental eggs. If these birds witness parasitism events, they may recognize and reject foreign eggs more readily than parents who did not. We found that, after being flushed, female blackbirds, Turdus merula, remained close to their nests. Flushed females were more likely to eject foreign eggs and did so more quickly than females that were not flushed during experimentation. In contrast, flushing did not predict responses and latency to responses to parasitism by song thrush, Turdus philomelos, which flew farther from their nests and likely did not witness experimental parasitism. When statistically considering flushing, previously published conclusions regarding both species' response to experimental parasitism did not change. Nevertheless, we recommend that researchers record and statistically control for whether hosts were flushed prior to experimental parasitism. Our results have broad implications because more vigilant and/or bolder parents can gain more information about parasitism events and therefore have better chances of successfully defending against brood parasitism. PMID:25762433

  20. Now you see it, now you don't: flushing hosts prior to experimentation can predict their responses to brood parasitism.

    PubMed

    Hanley, Daniel; Samaš, Peter; Heryán, Josef; Hauber, Mark E; Grim, Tomáš

    2015-01-01

    Brood parasitic birds lay their eggs in other birds' nests, leaving hosts to raise their offspring. To understand parasite-host coevolutionary arms races, many studies have examined host responses to experimentally introduced eggs. However, attending parents often need to be flushed from their nests to add experimental eggs. If these birds witness parasitism events, they may recognize and reject foreign eggs more readily than parents who did not. We found that, after being flushed, female blackbirds, Turdus merula, remained close to their nests. Flushed females were more likely to eject foreign eggs and did so more quickly than females that were not flushed during experimentation. In contrast, flushing did not predict responses and latency to responses to parasitism by song thrush, Turdus philomelos, which flew farther from their nests and likely did not witness experimental parasitism. When statistically considering flushing, previously published conclusions regarding both species' response to experimental parasitism did not change. Nevertheless, we recommend that researchers record and statistically control for whether hosts were flushed prior to experimental parasitism. Our results have broad implications because more vigilant and/or bolder parents can gain more information about parasitism events and therefore have better chances of successfully defending against brood parasitism. PMID:25762433

  1. Present-day plate motions

    NASA Technical Reports Server (NTRS)

    Minster, J. B.; Jordan, T. H.

    1977-01-01

    A data set comprising 110 spreading rates, 78 transform fault azimuths and 142 earthquake slip vectors was inverted to yield a new instantaneous plate motion model, designated RM2. The mean averaging interval for the relative motion data was reduced to less than 3 My. A detailed comparison of RM2 with angular velocity vectors which best fit the data along individual plate boundaries indicates that RM2 performs close to optimally in most regions, with several notable exceptions. On the other hand, a previous estimate (RM1) failed to satisfy an extensive set of new data collected in the South Atlantic Ocean. It is shown that RM1 incorrectly predicts the plate kinematics in the South Atlantic because the presently available data are inconsistent with the plate geometry assumed in deriving RM1. It is demonstrated that this inconsistency can be remedied by postulating the existence of internal deformation with the Indian plate, although alternate explanations are possible.

  2. Experimental Shifts in Intraclutch Egg Color Variation Do Not Affect Egg Rejection in a Host of a Non-Egg-Mimetic Avian Brood Parasite

    PubMed Central

    Croston, Rebecca; Hauber, Mark E.

    2015-01-01

    Avian brood parasites lay their eggs in the nests of other birds, and impose the costs associated with rearing parasitic young onto these hosts. Many hosts of brood parasites defend against parasitism by removing foreign eggs from the nest. In systems where parasitic eggs mimic host eggs in coloration and patterning, extensive intraclutch variation in egg appearances may impair the host’s ability to recognize and reject parasitic eggs, but experimental investigation of this effect has produced conflicting results. The cognitive mechanism by which hosts recognize parasitic eggs may vary across brood parasite hosts, and this may explain variation in experimental outcome across studies investigating egg rejection in hosts of egg-mimicking brood parasites. In contrast, for hosts of non-egg-mimetic parasites, intraclutch egg color variation is not predicted to co-vary with foreign egg rejection, irrespective of cognitive mechanism. Here we tested for effects of intraclutch egg color variation in a host of nonmimetic brood parasite by manipulating egg color in American robins (Turdus migratorius), hosts of brown-headed cowbirds (Molothrus ater). We recorded robins’ behavioral responses to simulated cowbird parasitism in nests where color variation was artificially enhanced or reduced. We also quantified egg color variation within and between unmanipulated robin clutches as perceived by robins themselves using spectrophotometric measures and avian visual modeling. In unmanipulated nests, egg color varied more between than within robin clutches. As predicted, however, manipulation of color variation did not affect rejection rates. Overall, our results best support the scenario wherein egg rejection is the outcome of selective pressure by a nonmimetic brood parasite, because robins are efficient rejecters of foreign eggs, irrespective of the color variation within their own clutch. PMID:25831051

  3. Dyes in Liquid Crystals: Experimental and Computational Studies of a Guest–Host System Based on a Combined DFT and MD Approach

    PubMed Central

    Sims, Mark T; Abbott, Laurence C; Cowling, Stephen J; Goodby, John W; Moore, John N

    2015-01-01

    Practical applications of guest–host liquid crystal systems are critically dependent on the alignment of the guest species within the liquid crystal host. UV/Vis absorption spectroscopy shows that the 1,5-dihydroxy-2,6-bis-(4-propylphenyl)-9,10-anthraquinone dye aligns within the E7 nematic host, giving an experimental dichroic ratio of 9.40 and dye order parameter of 0.74. This alignment was modelled by using a combination of density functional theory (DFT) and molecular dynamics (MD) computational approaches that do not require the input of experimental data. Time-dependent DFT calculations show that the electronic transition dipole moment is highly aligned with the long molecular axis of the dye. Fully atomistic MD simulations show that the long axis of the dye is less highly aligned within the E7 host, indicating that this contribution limits the overall dye alignment and, thereby, the potential practical applications of this particular system. Importantly, this study demonstrates an experimental and combined DFT and MD computational approach that may be applied generally to guest–host systems, providing a potential route to their rational design. PMID:26031244

  4. Experimental and Theoretical Demonstration on the Transport Properties of Fused Ring Host Materials for Organic Light-Emitting Diodes

    NASA Astrophysics Data System (ADS)

    Tse, S. C.; So, S. K.; Yeung, M. Y.; Lo, C. F.; Wen, S. W.; Chen, C. H.

    2006-01-01

    The charge transport properties of three tertiary-butyl (t-Bu) substituted anthracene derivatives (ADN), critical blue host materials for organic light-emitting diodes (OLEDs), have been investigated experimentally and computationally. From time-of-flight (TOF) measurements, all ADN compounds exhibit ambipolar characters. The hole and electron mobilities are in the range (1--5)× 10-7 cm2 V-1 s-1 under an external applied field of about 1 MV cm-1. Un-substituted ADN has the highest carrier mobilities while heavily t-Bu substituted ADN has the least. The electron and hole conducting properties of are consistent with ab initio calculation, which indicates that the frontier orbitals are localized mainly on the anthracene moiety. t-Bu substitutions in ADN increase the hopping path lengths among the molecules and hence reduce the electron and hole mobilities. The results demonstrate that t-Bu substitution is an effective means of engineering the conductivity of organic charge transporter for OLED applications.

  5. [Present-day 90Sr and 137Cs contamination levels of soil and agricultural products in the East-Urals Radioactive Trace area].

    PubMed

    Kazachenok, N N; Popova, I Ia; Kostiuchenko, V A; Mel'nikov, V S; Usol'tsev, D V

    2009-01-01

    Data represent present-day 90Sr and 137Cs contamination levels of soil and agricultural products (grain, vegetables and forage crops, milk and meat) in the East-Urals Radioactive Trace area, and the accumulation coefficients of these radionuclides in cash crop.

  6. Estimations of historical atmospheric mercury concentrations from mercury refining and present-day soil concentrations of total mercury in Huancavelica, Peru.

    PubMed

    Robins, Nicholas A; Hagan, Nicole; Halabi, Susan; Hsu-Kim, Heileen; Gonzales, Ruben Dario Espinoza; Morris, Mark; Woodall, George; Richter, Daniel deB; Heine, Paul; Zhang, Tong; Bacon, Allan; Vandenberg, John

    2012-06-01

    Detailed Spanish records of cinnabar mining and mercury production during the colonial period in Huancavelica, Peru were examined to estimate historical health risks to the community from exposure to elemental mercury (Hg) vapor resulting from cinnabar refining operations. Between 1564 and 1810, nearly 17,000 metric tons of Hg were released to the atmosphere in Huancavelica from Hg production. AERMOD was used with estimated emissions and source characteristics to approximate historic atmospheric concentrations of mercury vapor. Modeled 1-hour and long-term concentrations were compared with present-day inhalation reference values for elemental Hg. Estimated 1-hour maximum concentrations for the entire community exceeded present-day occupational inhalation reference values, while some areas closest to the smelters exceeded present-day emergency response guideline levels. Estimated long-term maximum concentrations for the entire community exceeded the EPA Reference Concentration (RfC) by a factor of 30 to 100, with areas closest to the smelters exceeding the RfC by a factor of 300 to 1000. Based on the estimated historical concentrations of Hg vapor in the community, the study also measured the extent of present-day contamination throughout the community through soil sampling and analysis. Total Hg in soils sampled from 20 locations ranged from 1.75 to 698 mg/kg and three adobe brick samples ranging from 47.4 to 284 mg/kg, consistent with other sites of mercury mining and use. The results of the soil sampling indicate that the present-day population of Huancavelica is exposed to levels of mercury from legacy contamination which is currently among the highest worldwide, consequently placing them at potential risk of adverse health outcomes. PMID:22542225

  7. Consistent pattern of local adaptation during an experimental heat wave in a pipefish-trematode host-parasite system.

    PubMed

    Landis, Susanne H; Kalbe, Martin; Reusch, Thorsten B H; Roth, Olivia

    2012-01-01

    Extreme climate events such as heat waves are expected to increase in frequency under global change. As one indirect effect, they can alter magnitude and direction of species interactions, for example those between hosts and parasites. We simulated a summer heat wave to investigate how a changing environment affects the interaction between the broad-nosed pipefish (Syngnathus typhle) as a host and its digenean trematode parasite (Cryptocotyle lingua). In a fully reciprocal laboratory infection experiment, pipefish from three different coastal locations were exposed to sympatric and allopatric trematode cercariae. In order to examine whether an extreme climatic event disrupts patterns of locally adapted host-parasite combinations we measured the parasite's transmission success as well as the host's adaptive and innate immune defence under control and heat wave conditions. Independent of temperature, sympatric cercariae were always more successful than allopatric ones, indicating that parasites are locally adapted to their hosts. Hosts suffered from heat stress as suggested by fewer cells of the adaptive immune system (lymphocytes) compared to the same groups that were kept at 18°C. However, the proportion of the innate immune cells (monocytes) was higher in the 18°C water. Contrary to our expectations, no interaction between host immune defence, parasite infectivity and temperature stress were found, nor did the pattern of local adaptation change due to increased water temperature. Thus, in this host-parasite interaction, the sympatric parasite keeps ahead of the coevolutionary dynamics across sites, even under increasing temperatures as expected under marine global warming.

  8. Temporal-spatial pathological changes in the brains of permissive and non-permissive hosts experimentally infected with Angiostrongylus cantonensis.

    PubMed

    Wang, Lian-Chen; Jung, Shih-Ming; Chen, Kuang-Yao; Wang, Tzu-Yi; Li, Chung-Han

    2015-10-01

    Human cerebral angiostrongyliasis becomes an emerging disease in many parts of the world. By postmortem examination, Angiostrongylus cantonensis have been reported to cause severe pathological changes in the central nervous system. The present study was designed to determine the temporal-spatial pathological changes through experimental infections and histopathological examination of permissive (SD rats) and non-permissive (ICR mice) hosts. After infecting SD rats with 25, 50, or 100 third-stage larvae (L3) and ICR mice with 25 L3, one animal from each group was sacrificed daily from day 1 to day 30 post-infection. Each rat brain was cut into six sections and mouse brain into five sections. These sections were stained with haematoxylin and eosin and examined microscopically. Eosinophilic meningitis was found to be the most commonly pathological change and occurred on day 17 post-infection in rats with 25 L3, day 9 in the 50- or 100-L3 groups, and day 12 in infected mice. Thickness of the meninges increased 9-24 folds in infected rats and 89 folds in an infected mouse on day 28. Encephalitis, congestion, perivascular cuffing, and haemorrhage were revealed in infected mice and rats with 100 L3. Fifth-stage larvae were frequently observed in the meninges but occasionally in the parenchyma. Significant correlations between meningitis and presence of larvae in the meninges were found in the three infected rat groups but not in the infected mice. The results indicate that the clinical course of A. cantonensis infection is not self-limited but becomes more severe with the intensity of infection.

  9. Temporal-spatial pathological changes in the brains of permissive and non-permissive hosts experimentally infected with Angiostrongylus cantonensis.

    PubMed

    Wang, Lian-Chen; Jung, Shih-Ming; Chen, Kuang-Yao; Wang, Tzu-Yi; Li, Chung-Han

    2015-10-01

    Human cerebral angiostrongyliasis becomes an emerging disease in many parts of the world. By postmortem examination, Angiostrongylus cantonensis have been reported to cause severe pathological changes in the central nervous system. The present study was designed to determine the temporal-spatial pathological changes through experimental infections and histopathological examination of permissive (SD rats) and non-permissive (ICR mice) hosts. After infecting SD rats with 25, 50, or 100 third-stage larvae (L3) and ICR mice with 25 L3, one animal from each group was sacrificed daily from day 1 to day 30 post-infection. Each rat brain was cut into six sections and mouse brain into five sections. These sections were stained with haematoxylin and eosin and examined microscopically. Eosinophilic meningitis was found to be the most commonly pathological change and occurred on day 17 post-infection in rats with 25 L3, day 9 in the 50- or 100-L3 groups, and day 12 in infected mice. Thickness of the meninges increased 9-24 folds in infected rats and 89 folds in an infected mouse on day 28. Encephalitis, congestion, perivascular cuffing, and haemorrhage were revealed in infected mice and rats with 100 L3. Fifth-stage larvae were frequently observed in the meninges but occasionally in the parenchyma. Significant correlations between meningitis and presence of larvae in the meninges were found in the three infected rat groups but not in the infected mice. The results indicate that the clinical course of A. cantonensis infection is not self-limited but becomes more severe with the intensity of infection. PMID:26299243

  10. Post World War II orcharding creates present day DDT-problems in The Sørfjord (Western Norway)--a case study.

    PubMed

    Ruus, Anders; Green, Norman W; Maage, Amund; Amundsen, Carl Einar; Schøyen, Merete; Skei, Jens

    2010-10-01

    The Sørfjord has a long history of agriculture and industry, and environmental monitoring has been conducted for decades, comprising analyses of contaminants in mussel, fish and sediments. DDT was used as an insecticide in orchards surrounding the fjord between World War II and 1970. Since the early 1990 s, elevated concentrations of DDT were found in mussels and fish. Unexpectedly, DDT-concentrations increased towards present day, despite the discontinuation of use. The highest concentrations in mussels (in 2006) corresponded to about two orders of magnitude higher than background. Analyses of sediment core sections also indicated increased input towards present day. Shifts in climatic parameters, as well as increased amounts of soil dissolved organic carbon following a decline in atmospheric sulphate deposition may have contributed to this phenomenon. We warrant the need for increased knowledge of the effects of alterations in variables acting regionally and globally on the disposition of contaminants in ecosystems.

  11. Genetic structure of Flores island (Azores, Portugal) in the 19th century and in the present day: evidence from surname analysis.

    PubMed

    Santos, Cristina; Abade, Augusto; Cantons, Jordi; Mayer, Francine M; Aluja, M Pilar; Lima, Manuela

    2005-06-01

    The island of Flores is the most westerly of the Azores archipelago (Portugal). Despite its marked geographic isolation and reduced population size, biodemographic and genetic studies conducted so far do not support the idea that its population constitutes a genetic isolate. In this study we conducted a surname analysis of the Flores population for two time periods: the second half of the 19th century and the present day. Our main purposes were (1) to biodemographically and genetically characterize the island, taking into account the strong reduction in population observed from the middle of the 19th century to the present day; and (2) to analyze the influence that the effective population size and geographic distance have on the genetic structure of populations. For both periods analyzed, all indicators of diversity revealed a high level of surname diversity. Our results are in accordance with the diversity estimates obtained from both monoparental genetic markers located in the Y chromosome and frequencies of mtDNA haplogroups. Contrary to what could be expected, considering the strong reduction of population in the last 150 years, we observed that diversity was maintained and that microdifferentiation decreased. Both observations support a higher openness of parishes as a consequence of the increase in communication routes. From the first to the second period analyzed, a change in surname composition is evident, although the more frequent surnames in Flores are almost the same for both periods and some of them are reported to be surnames present in the first settlers of Flores. This result testifies to the impact of founders on the present-day gene pool of Flores island and allows us to infer that the genetic characterization of the present-day population of Flores could provide reliable information about the history of the peopling of the Azores.

  12. Simulation of the present-day atmospheric ozone, odd nitrogen, chlorine and other species using a coupled 2-D model in isentropic coordinates

    NASA Technical Reports Server (NTRS)

    Yang, H.; Olaguer, E.; Tung, K. K.

    1991-01-01

    A zonally averaged two-dimensional model for chemical species in the atmosphere (Olaguer et al., 1990), with coupled dynamics, radiation transfer, and chemistry, is used to study the present-day atmospheric ozone, odd nitrogen, chlorine, other species. The model utilizes all zonally averaged physical equations of momentum, energy, and mass and determines self-consistently the advective and diffusive tranasport parameters from the temperature specific to the period of observation. A comparison of the model results with observations showed good agreement.

  13. Genetic structure of Flores island (Azores, Portugal) in the 19th century and in the present day: evidence from surname analysis.

    PubMed

    Santos, Cristina; Abade, Augusto; Cantons, Jordi; Mayer, Francine M; Aluja, M Pilar; Lima, Manuela

    2005-06-01

    The island of Flores is the most westerly of the Azores archipelago (Portugal). Despite its marked geographic isolation and reduced population size, biodemographic and genetic studies conducted so far do not support the idea that its population constitutes a genetic isolate. In this study we conducted a surname analysis of the Flores population for two time periods: the second half of the 19th century and the present day. Our main purposes were (1) to biodemographically and genetically characterize the island, taking into account the strong reduction in population observed from the middle of the 19th century to the present day; and (2) to analyze the influence that the effective population size and geographic distance have on the genetic structure of populations. For both periods analyzed, all indicators of diversity revealed a high level of surname diversity. Our results are in accordance with the diversity estimates obtained from both monoparental genetic markers located in the Y chromosome and frequencies of mtDNA haplogroups. Contrary to what could be expected, considering the strong reduction of population in the last 150 years, we observed that diversity was maintained and that microdifferentiation decreased. Both observations support a higher openness of parishes as a consequence of the increase in communication routes. From the first to the second period analyzed, a change in surname composition is evident, although the more frequent surnames in Flores are almost the same for both periods and some of them are reported to be surnames present in the first settlers of Flores. This result testifies to the impact of founders on the present-day gene pool of Flores island and allows us to infer that the genetic characterization of the present-day population of Flores could provide reliable information about the history of the peopling of the Azores. PMID:16392635

  14. Formation of hyperextended rifted margins: Insights from flexural isostatic structural-stratigraphic modeling and observations from present-day rifted margins

    NASA Astrophysics Data System (ADS)

    Mohn, Geoffroy; Kusznir, Nick; Manatschal, Gianreto

    2014-05-01

    The understanding of how continental lithosphere extends, thins and ruptures leading to the formation of a new divergent plate boundary represent a fundamental question in Earth Sciences. In particular the mechanisms controlling the extreme pre-breakup stretching and thinning of the continental crust and lithosphere, documented at many present-day rifted margins, are still poorly known. Many questions remain of the fundamental processes controlling the extensional deformation of the continental crust and lithosphere, including fault geometries and their evolution in space and time, the occurrence of decoupling horizons within the continental crust and the importance of depth-dependent lithosphere thinning processes. We investigate the control of these key factors on continental crust and lithosphere thinning processes by combining seismic reflection and drill-hole observations from present-day Iberia-Newfoundland rifted margins with flexural isostatic forward tectonic and stratigraphic modeling. These observations made at present-day rifted margins constrain the input parameters used in the flexural isostatic forward modeling. At the same time, the forward tectonic and stratigraphic modeling provides validation of the interpretation of the seismic reflection data. Through this modeling, we produce isostatically and thermally balanced sections reproducing the geometries observed along the Iberia-Newfoundland rifted margins. Our results suggest that crustal and lithospheric thinning results from the combination of both pure- and simple-shear deformation. The model predicts the critical role of intra-crustal decoupling horizons confirming the importance of depth-dependent thinning through polyphased rifting events.

  15. The influence of dynamic vegetation on the present-day simulation and future projections of the South Asian summer monsoon in the HadGEM2 family

    NASA Astrophysics Data System (ADS)

    Martin, G. M.; Levine, R. C.

    2012-11-01

    Various studies have shown the importance of Earth System feedbacks in the climate system and the necessity of including these in models used for making climate change projections. The HadGEM2 family of Met Office Unified Model configurations combines model components which facilitate the representation of many different processes within the climate system, including atmosphere, ocean and sea ice, and Earth System components including the terrestrial and oceanic carbon cycle and tropospheric chemistry. We examine the climatology of the Asian summer monsoon in present-day simulations and in idealised climate change experiments. Members of the HadGEM2 family are used, with a common physical framework (one of which includes tropospheric chemistry and an interactive terrestrial and oceanic carbon cycle), to investigate whether such components affect the way in which the monsoon changes. We focus particularly on the role of interactive vegetation in the simulations from these model configurations. Using an atmosphere-only HadGEM2 configuration, we investigate how the changes in land cover which result from the interaction between the dynamic vegetation and the model systematic rainfall biases affect the Asian summer monsoon, both in the present-day and in future climate projections. We demonstrate that the response of the dynamic vegetation to biases in regional climate, such as lack of rainfall over tropical dust-producing regions, can affect both the present-day simulation and the response to climate change forcing scenarios.

  16. The influence of dynamic vegetation on the present-day simulation and future projections of the South Asian summer monsoon in the HadGEM2 family

    NASA Astrophysics Data System (ADS)

    Martin, G. M.; Levine, R. C.

    2012-08-01

    Various studies have shown the importance of Earth System feedbacks in the climate system and the necessity of including these in models used for making climate change projections. The HadGEM2 family of Met Office Unified Model configurations combines model components which facilitate the representation of many different processes within the climate system, including atmosphere, ocean and sea ice, and Earth System components including the terrestrial and oceanic carbon cycle and tropospheric chemistry. We examine the climatology of the Asian summer monsoon in present-day simulations and in idealised climate change experiments in which a quadrupling of CO2 is applied as a step change. Members of the HadGEM2 family are used, with a common physical framework, one of which includes tropospheric chemistry and an interactive terrestrial and oceanic carbon cycle, to investigate whether such components affect the way in which the monsoon changes. We focus particularly on the role of interactive vegetation in the simulations from these model configurations. Using an atmosphere-only HadGEM2 configuration, we investigate how the changes in land cover which result from the interaction between the dynamic vegetation and the model systematic rainfall biases affect the Asian summer monsoon, both in the present-day and in future climate projections. We demonstrate that the response of the dynamic vegetation to biases in regional climate, such as lack of rainfall over tropical dust-producing regions, can affect both the present-day simulation and the response to climate change forcing scenarios.

  17. Using GPS and Absolute Gravity Observations to Separate the Effects of Present-day and Pleistocene Ice-mass Changes in South East Greenland

    NASA Astrophysics Data System (ADS)

    van Dam, T. M.; Francis, O.; Wahr, J. M.; Khan, S. A.; Bevis, M. G.; van den Broeke, M.

    2015-12-01

    Precise estimates of the present-day rates and accelerations of ice-mass loss from the polar ice-sheets are important for predicting potential changes in sea level. Current predictions of 21st century sea level change are limited by, among other things, their ability to precisely capture the effects of global warming on the Greenland ice sheet (GrIS). One method for constraining the mass loss on the GrIS is to make measurements of vertical crustal uplift rates from bedrock around the edge of the ice sheet. Using only GPS observations of crustal displacement, it is impossible to separate the uplift driven by present day mass changes from that due to ice mass changes in the past, e.g. the extensive retreat of the ice sheets since the Last Glacial Maximum (LGM) or even ice sheet changes during the Little Ice Age. By making measurements of both gravity and surface motion at a bedrock site, the viscoelastic effects could be removed from the observations and we would be able to constrain present day ice mass changes. In this presentation we discuss the results of an experiment to collect surface displacement and absolute gravity observations from southeast Greenland to separate the elastic and viscoelastic signals. We find that the glacial isostatic adjustment signal in this region is positive, contrary to the negative signal predicted by all existing viscosity and ice history models.

  18. Preindustrial to Present-Day Changes in Tropospheric Hydroxyl Radical and Methane Lifetime from the Atmospheric Chemistry and Climate Model Intercomparison Project (ACCMIP)

    NASA Technical Reports Server (NTRS)

    Naik, V.; Voulgarakis, A.; Fiore, A. M.; Horowitz, L. W.; Lamarque, J.-F.; Lin, M.; Prather, M. J.; Young, P. J.; Bergmann, D.; Cameron-Smith, P. J.; Cionni, I.; Collins, W. J.; Dalsoren, S. B.; Doherty, R.; Eyring, V.; Faluvegi, G.; Folberth, G. A.; Josse, B.; Lee, Y. H.; MacKenzie, I. A.; Nagashima, T.; vanNoije, T. P. C.; Plummer, D. A.; Righi, M.; Rumbold, S. T.; Skeie, R.; Shindell, D. T.; Stevenson, D. S.; Strode, S.; Sudo, K.; Szopa, S.; Zeng, G.

    2013-01-01

    We have analysed time-slice simulations from 17 global models, participating in the Atmospheric Chemistry and Climate Model Intercomparison Project (ACCMIP), to explore changes in present-day (2000) hydroxyl radical (OH) concentration and methane (CH4) lifetime relative to preindustrial times (1850) and to 1980. A comparison of modeled and observation-derived methane and methyl chloroform lifetimes suggests that the present-day global multi-model mean OH concentration is overestimated by 5 to 10% but is within the range of uncertainties. The models consistently simulate higher OH concentrations in the Northern Hemisphere (NH) compared with the Southern Hemisphere (SH) for the present-day (2000; inter-hemispheric ratios of 1.13 to 1.42), in contrast to observation-based approaches which generally indicate higher OH in the SH although uncertainties are large. Evaluation of simulated carbon monoxide (CO) concentrations, the primary sink for OH, against ground-based and satellite observations suggests low biases in the NH that may contribute to the high north–south OH asymmetry in the models. The models vary widely in their regional distribution of present-day OH concentrations (up to 34%). Despite large regional changes, the multi-model global mean (mass-weighted) OH concentration changes little over the past 150 yr, due to concurrent increases in factors that enhance OH (humidity, tropospheric ozone, nitrogen oxide (NOx) emissions, and UV radiation due to decreases in stratospheric ozone), compensated by increases in OH sinks (methane abundance, carbon monoxide and non-methane volatile organic carbon (NMVOC) emissions). The large inter-model diversity in the sign and magnitude of preindustrial to present-day OH changes (ranging from a decrease of 12.7% to an increase of 14.6%) indicate that uncertainty remains in our understanding of the long-term trends in OH and methane lifetime. We show that this diversity is largely explained by the different ratio of the

  19. Experimental infection studies demonstrating Atlantic salmon as a host and reservoir of viral hemorrhagic septicemia virus type IVa with insights into pathology and host immunity

    USGS Publications Warehouse

    Lovy, Jan; Piesik, P.; Hershberger, P.K.; Garver, K.A.

    2013-01-01

    In British Columbia, Canada (BC), aquaculture of finfish in ocean netpens has the potential for pathogen transmission between wild and farmed species due to the sharing of an aquatic environment. Viral hemorrhagic septicemia virus (VHSV) is enzootic in BC and causes serious disease in wild Pacific herring, Clupea pallasii, which often enter and remain in Atlantic salmon, Salmo salar, netpens. Isolation of VHSV from farmed Atlantic salmon has been previously documented, but the effects on the health of farmed salmon and the wild fish sharing the environment are unknown. To determine their susceptibility, Atlantic salmon were exposed to a pool of 9 isolates of VHSV obtained from farmed Atlantic salmon in BC by IP-injection or by waterborne exposure and cohabitation with diseased Pacific herring. Disease intensity was quantified by recording mortality, clinical signs, histopathological changes, cellular sites of viral replication, expression of interferon-related genes, and viral tissue titers. Disease ensued in Atlantic salmon after both VHSV exposure methods. Fish demonstrated gross disease signs including darkening of the dorsal skin, bilateral exophthalmia, light cutaneous hemorrhage, and lethargy. The virus replicated within endothelial cells causing endothelial cell necrosis and extensive hemorrhage in anterior kidney. Infected fish demonstrated a type I interferon response as seen by up-regulation of genes for IFNα, Mx, and ISG15. In a separate trial infected salmon transmitted the virus to sympatric Pacific herring. The results demonstrate that farmed Atlantic salmon can develop clinical VHS and virus can persist in the tissues for at least 10 weeks. Avoiding VHS epizootics in Atlantic salmon farms would limit the potential of VHS in farmed Atlantic salmon, the possibility for further host adaptation in this species, and virus spillback to sympatric wild fishes.

  20. Estimating heat stress from climate-based indicators: present-day biases and future spreads in the CMIP5 global climate model ensemble

    NASA Astrophysics Data System (ADS)

    Zhao, Y.; Ducharne, A.; Sultan, B.; Braconnot, P.; Vautard, R.

    2015-08-01

    The increased exposure of human populations to heat stress is one of the likely consequences of global warming, and it has detrimental effects on health and labor capacity. Here, we consider the evolution of heat stress under climate change using 21 general circulation models (GCMs). Three heat stress indicators, based on both temperature and humidity conditions, are used to investigate present-day model biases and spreads in future climate projections. Present day estimates of heat stress indicators from observational data shows that humid tropical areas tend to experience more frequent heat stress than other regions do, with a total frequency of heat stress 250-300 d yr-1. The most severe heat stress is found in the Sahel and south India. Present-day GCM simulations tend to underestimate heat stress over the tropics due to dry and cold model biases. The model based estimates are in better agreement with observation in mid to high latitudes, but this is due to compensating errors in humidity and temperature. The severity of heat stress is projected to increase by the end of the century under climate change scenario RCP8.5, reaching unprecedented levels in some regions compared with observations. An analysis of the different factors contributing to the total spread of projected heat stress shows that spread is primarily driven by the choice of GCMs rather than the choice of indicators, even when the simulated indicators are bias-corrected. This supports the utility of the multi-model ensemble approach to assess the impacts of climate change on heat stress.

  1. Comment on ``A new estimate for present-day Cocos-Caribbean plate motion: Implications for slip along the Central American volcanic arc'' by Charles DeMets

    NASA Astrophysics Data System (ADS)

    Guzmán-Speziale, Marco; Gómez, Juan Martín

    2002-10-01

    We comment on ``A new estimate for present-day Cocos-Caribbean plate motion: Implications for slip along the Central American volcanic arc'' by Charles DeMets. We find the following inconsistencies in his model: Components of relative motion along the arc are small and variable, not uniform. There is no single surface faulting and earthquakes occur on faults along and perpendicular to the arc. Earthquakes also stop in the middle of the arc. Geometrically, the model calls for buttressing, but there is no evidence for this.

  2. Research in radiobiology: Final report of work in progress in immunobiology of experimental host-tumor relationships

    SciTech Connect

    Not Available

    1993-03-15

    Our work on the immunobiology of tumors induced in normal mice by non-ionizing radiation and chemical carcinogens has previously demonstrated a correlation between MHC molecule expression and the immunogenicity of tumors in a transplanted syngeneic host. Such that immunogenic or regressive tumors were found to demonstrate higher constitutive or inducible levels of MHC expression, while most virulent, aggressive tumors exhibited a low level of MHC Class I expression. We attributed much of the control of MHC molecule expression by antigen-bearing tumors and normal cells to the immunological status of the host since the host must provide the appropriate stimulus to enhance MHC antigen expression by the invading tumor. Our results with UVR-induced tumors suggested that a significant role is played by the T-cell lymphokine, {gamma}-interferon ({gamma}IFN), in the modulation of MHC molecule expression in vivo. Virulent tumors, induced by boneseeking radionuclides, may be refractory to {gamma}IFN stimulation of MHC molecule expression. It is also possible that certain tumors might be fully responsive to the Class I modulatory influences by {gamma}IFN, but exhibit a reduced capacity to stimulate the synthesis of this lymphokine by host T cells. We present experiments designed to : Describe the virulence, latency period, and transplantation characteristics of {sup 238}PU, {sup 24l}Am, and {sup 228}Th tumors arising as osteogenic sarcomas and hepatic carcinomas, to determine the relationship between inducible expression of MHC Class I molecules by {gamma}IFN and in vivo immunogenicity of these radioisotype-induced tumors, and to elucidate any molecular mechanisms responsible for a lack of responsiveness to a {gamma}IFN failure by the host to induce host {gamma}IFN production.

  3. Research in radiobiology: Final report of work in progress in immunobiology of experimental host-tumor relationships

    SciTech Connect

    Not Available

    1993-03-15

    Our work on the immunobiology of tumors induced in normal mice by non-ionizing radiation and chemical carcinogens has previously demonstrated a correlation between MHC molecule expression and the immunogenicity of tumors in a transplanted syngeneic host. Such that immunogenic or regressive tumors were found to demonstrate higher constitutive or inducible levels of MHC expression, while most virulent, aggressive tumors exhibited a low level of MHC Class I expression. We attributed much of the control of MHC molecule expression by antigen-bearing tumors and normal cells to the immunological status of the host since the host must provide the appropriate stimulus to enhance MHC antigen expression by the invading tumor. Our results with UVR-induced tumors suggested that a significant role is played by the T-cell lymphokine, [gamma]-interferon ([gamma]IFN), in the modulation of MHC molecule expression in vivo. Virulent tumors, induced by boneseeking radionuclides, may be refractory to [gamma]IFN stimulation of MHC molecule expression. It is also possible that certain tumors might be fully responsive to the Class I modulatory influences by [gamma]IFN, but exhibit a reduced capacity to stimulate the synthesis of this lymphokine by host T cells. We present experiments designed to : Describe the virulence, latency period, and transplantation characteristics of [sup 238]PU, [sup 24l]Am, and [sup 228]Th tumors arising as osteogenic sarcomas and hepatic carcinomas, to determine the relationship between inducible expression of MHC Class I molecules by [gamma]IFN and in vivo immunogenicity of these radioisotype-induced tumors, and to elucidate any molecular mechanisms responsible for a lack of responsiveness to a [gamma]IFN failure by the host to induce host [gamma]IFN production.

  4. [The specific features of present-day children's physical development in the estimation of the functional sizes of furniture for pupils].

    PubMed

    Khramtsov, P I; Strokina, A N; Sotnikova, E N; Butareva, I I; Moldovanov, V V

    2009-01-01

    The authors made mass anthropometric surveys in 923 first-to-fourth-form pupils and determined the values of 5 variables for height groups 2, 3, and 4, used to justify the functional sizes of furniture for pupils: the length of a shoulder slope above the seat, that of an elbow slope above the seat, that of a popliteal space slope above the floor, the distance from the chair hack to the popliteal space, and the highest pelvic width. Differences were found in the anthropometric values in the present-day junior pupils and the equals in age of the early 1970s. The present-day children are characterized by changes in body proportions (a decrease in height and an increase in the length of the shin and femur), which should be kept in mind on optimizing the working place of pupils. It is suggested that popliteal space length rather than the currently applied height should be used as a fitting ratio of anthropometric characteristics to the functional sizes of furniture for pupils.

  5. Morphological indicators of growth stages in carbonates platform evolution: comparison between present-day and Miocene platforms of Northern Borneo, Malaysia.

    NASA Astrophysics Data System (ADS)

    Pierson, B.; Menier, D.; Ting, K. K.; Chalabi, A.

    2012-04-01

    Satellite images of present-day reefs and carbonate platforms of the Celebes Sea, east of Sabah, Malaysia, exhibit large-scale features indicative of the recent evolution of the platforms. These include: (1) multiple, sub-parallel reef rims at the windward margin, suggestive of back-stepping of the platform margin; (2) contraction of the platform, possibly as a result of recent sea level fluctuations; (3) colonization of the internal lagoons by polygonal reef structures and (4) fragmentation of the platforms and creation of deep channels separating platforms that used to be part of a single entity. These features are analogue to what has been observed on seismic attribute maps of Miocene carbonate platforms of Sarawak. An analysis of several growth stages of a large Miocene platform, referred to as the Megaplatform, shows that the platform evolves in function of syn-depositional tectonic movements and sea level fluctuations that result in back-stepping of the margin, illustrated by multiple reef rims, contraction of the platform, the development of polygonal structures currently interpreted as karstic in origin and fragmentation of the megaplatform in 3 sub-entities separated by deep channels that precedes the final demise of the whole platform. Comparing similar features on present-day to platforms and Miocene platforms leads to a better understanding of the growth history of Miocene platforms and to a refined predictability of reservoir and non-reservoir facies distribution.

  6. [Association of the strA-strB genes with plasmids and transposons in the present-day bacteria and in bacterial strains from permafrost].

    PubMed

    Petrova, M A; Gorlenko, Zh M; Soina, V S; Mindlin, S Z

    2008-09-01

    Transposons closely related to the streptomycin resistance transposon of modem bacteria, Tn5393, were detected in the bacterial isolates from permafrost resistant to streptomycin. Many transposons studied were located on the medium-size plasmids with a narrow host range. None of the streptomycin-resistant strains isolated from permafrost contained small plasmids carrying the strA-strB genes and related to the broad host range plasmid RSF1010.

  7. Structural Evolution of the India-Arabia Plate Boundary from Miocene to Present-Day (NW Indian Ocean) and Comparison with the Dead Sea Fault (Eastern Mediterranean Sea).

    NASA Astrophysics Data System (ADS)

    Rodriguez, M.; Huchon, P.; Chamot Rooke, N.; Fournier, M.; Delescluse, M.; Ben Avraham, Z.; Ten Brink, U. S.

    2014-12-01

    Arabia is bounded by the Dead Sea Transform (DST) to the west and by the Owen Fracture Zone (OFZ) to the east. These present-day major strike-slip fault systems activated during the Plio-Pleistocene, which contrasts with the age of inception of strike-slip motion, assumed to begin around 13-18 Ma for the DST and around 20 Ma at the edge of the Owen-Murray Ridge (OMR) for the India-Arabia plate boundary. This discrepancy between the age of the active strike-slip systems and the age of inception of strike-slip motion raises the question of the kinematic driver for the transition between successive generations of strike-slip faults. Using a recent mutibeam and seismic dataset crossing the OFZ and the OMR, we provide a new geodynamic framework for the Miocene to present-day structural evolution of the India-Arabia plate boundary, and highlight some similarities with the structural evolution of the DST. We first document a Late Miocene episode of uplift of the OMR uplift along the Miocene India-Arabia plate boundary. The onset of this uplift is coeval with a plate reorganization event marked by the onset of intra-plate deformation in the Central Indian Ocean. The OFZ emplaced around 3 Ma, with major pull-apart basins opening (20°N Basin, Dalrymple Trough) dated at 2.4 Ma by far-field correlation with ODP Sites. The opening of pull-apart basins is coeval with the last structural reorganization of the Makran accretionnary wedge, marked by the regional M-unconformity, and with a major intensification of the Indian monsoon. A Late Miocene episode of folding is also recognized at the Lebanon ranges prior to the onset of the present-day DST, which occurred in the Late Pliocene-Early Pleistocene. The similarities between the geological history of the India-Arabia plate boundary and the DST in the Late Miocene and the Late Pliocene-Early Pleistocene suggest that both plate boundaries recorded the same kinematic changes. Late Miocene (i.e. Tortonian) deformation is widely

  8. Transient climate simulation from the Maunder Minimum to present day using prescribed changes in GHG, total/spectral solar irradiance and ozone

    NASA Astrophysics Data System (ADS)

    Spangehl, Thomas; Cubasch, Ulrich; Schimanke, Semjon

    A fully coupled AO-GCM including representation of the middle atmosphere is used for tran-sient simulation of climate from 1630 to 2000 AD. For better representation of changes in the UV/visible part of the solar spectrum an improved short-wave radiation scheme is implemented. The model is driven by changes in GHG concentrations, solar activity and volcanic eruptions. Solar variability is introduced via changes in total/spectral solar irradiance (TSI/SSI) and pre-scribed changes in stratospheric ozone. The secular trend in TSI is in the range of 0.1 percent increase from Maunder Minimum to present-day. Volcanic eruptions are represented via abrupt reduction in TSI. With the applied forcings the model does not simulate a clear reduction of the annual Northern Hemisphere (NH) mean near surface temperature during Maunder Minimum. By contrast the Dalton Minimum is characterized by distinct cooling and there is a significant raise of NH mean near surface temperature until the end of the 20th century. Focusing on the North Atlantic/European region the winter mean near surface temperature change pat-tern from Late Maunder Minimum (1675-1715) to present-day (1960-1990) reveals maximum warming over north-eastern Europe and cooling over the western North Atlantic with maxi-mum cooling west of Greenland. These changes can partly be explained by a shift of the NAO towards a more positive phase. The simulated changes in tropospheric circulation are discussed with special emphasize on the role of the solar forcing. Besides the stratospheric solar forcing which may affect NAO variability via downward propagation of the solar signal from the strato-sphere to the troposphere the magnitude of the secular trend in TSI might play a role. For the period from Maunder Minimum to present-day the simulation shows less near surface temper-ature increase especially over arctic regions when compared to simulations performed with the same model including the standard radiation scheme but

  9. Model for Vaccine Design by Prediction of B-Epitopes of IEDB Given Perturbations in Peptide Sequence, In Vivo Process, Experimental Techniques, and Source or Host Organisms

    PubMed Central

    González-Díaz, Humberto; Pérez-Montoto, Lázaro G.; Ubeira, Florencio M.

    2014-01-01

    Perturbation methods add variation terms to a known experimental solution of one problem to approach a solution for a related problem without known exact solution. One problem of this type in immunology is the prediction of the possible action of epitope of one peptide after a perturbation or variation in the structure of a known peptide and/or other boundary conditions (host organism, biological process, and experimental assay). However, to the best of our knowledge, there are no reports of general-purpose perturbation models to solve this problem. In a recent work, we introduced a new quantitative structure-property relationship theory for the study of perturbations in complex biomolecular systems. In this work, we developed the first model able to classify more than 200,000 cases of perturbations with accuracy, sensitivity, and specificity >90% both in training and validation series. The perturbations include structural changes in >50000 peptides determined in experimental assays with boundary conditions involving >500 source organisms, >50 host organisms, >10 biological process, and >30 experimental techniques. The model may be useful for the prediction of new epitopes or the optimization of known peptides towards computational vaccine design. PMID:24741624

  10. Evaluating CMIP5 ocean biogeochemistry and Southern Ocean carbon uptake using atmospheric potential oxygen: Present-day performance and future projection

    NASA Astrophysics Data System (ADS)

    Nevison, C. D.; Manizza, M.; Keeling, R. F.; Stephens, B. B.; Bent, J. D.; Dunne, J.; Ilyina, T.; Long, M.; Resplandy, L.; Tjiputra, J.; Yukimoto, S.

    2016-03-01

    Observed seasonal cycles in atmospheric potential oxygen (APO ~ O2 + 1.1 CO2) were used to evaluate eight ocean biogeochemistry models from the Coupled Model Intercomparison Project (CMIP5). Model APO seasonal cycles were computed from the CMIP5 air-sea O2 and CO2 fluxes and compared to observations at three Southern Hemisphere monitoring sites. Four of the models captured either the observed APO seasonal amplitude or phasing relatively well, while the other four did not. Many models had an unrealistic seasonal phasing or amplitude of the CO2 flux, which in turn influenced APO. By 2100 under RCP8.5, the models projected little change in the O2 component of APO but large changes in the seasonality of the CO2 component associated with ocean acidification. The models with poorer performance on present-day APO tended to project larger net carbon uptake in the Southern Ocean, both today and in 2100.

  11. Differential responses of calcifying and non-calcifying epibionts of a brown macroalga to present-day and future upwelling pCO2.

    PubMed

    Saderne, Vincent; Wahl, Martin

    2013-01-01

    Seaweeds are key species of the Baltic Sea benthic ecosystems. They are the substratum of numerous fouling epibionts like bryozoans and tubeworms. Several of these epibionts bear calcified structures and could be impacted by the high pCO2 events of the late summer upwellings in the Baltic nearshores. Those events are expected to increase in strength and duration with global change and ocean acidification. If calcifying epibionts are impacted by transient acidification as driven by upwelling events, their increasing prevalence could cause a shift of the fouling communities toward fleshy species. The aim of the present study was to test the sensitivity of selected seaweed macrofoulers to transient elevation of pCO2 in their natural microenvironment, i.e. the boundary layer covering the thallus surface of brown seaweeds. Fragments of the macroalga Fucus serratus bearing an epibiotic community composed of the calcifiers Spirorbis spirorbis (Annelida) and Electra pilosa (Bryozoa) and the non-calcifier Alcyonidium hirsutum (Bryozoa) were maintained for 30 days under three pCO2 conditions: natural 460 ± 59 µatm, present-day upwelling1193 ± 166 µatm and future upwelling 3150 ± 446 µatm. Only the highest pCO2 caused a significant reduction of growth rates and settlement of S. spirorbis individuals. Additionally, S. spirorbis settled juveniles exhibited enhanced calcification of 40% during daylight hours compared to dark hours, possibly reflecting a day-night alternation of an acidification-modulating effect by algal photosynthesis as opposed to an acidification-enhancing effect of algal respiration. E. pilosa colonies showed significantly increased growth rates at intermediate pCO2 (1193 µatm) but no response to higher pCO2. No effect of acidification on A. hirsutum colonies growth rates was observed. The results suggest a remarkable resistance of the algal macro-epibionts to levels of acidification occurring at present day upwellings in the Baltic. Only extreme

  12. Quantitative Study of the Present-Day Climate of the Middle Tennessee Elk Watershed Area From Global and Regional Climate Model Simulations

    NASA Astrophysics Data System (ADS)

    Kebede, G.

    2015-12-01

    As part of a wider hydro climatic modeling research, we studied the spatial and temporal variability of precipitation and temperature over the Middle Tennessee Elk watershed and its environs using regional climate model simulations over the past 30 years. Three sets of simulations with the Hadley Center's regional climate model (PRECIS) were carried out for the present day climate (1980-2010) at a resolution of 25km covering the southeastern U.S. These three sets simulations are driven by lateral boundary conditions taken from ERA-Interim reanalysis, and two global climate models (HadCM3 and ECHAM5) respectively. For validation, high resolution observed daily data sets from North American Land-Data Assimilation System (NLDAS) and Climate Research Unit, CRU data are used. Preliminary results show that the spatial distribution of the present-day seasonal mean rainfall and temperature, simulated by PRECIS, are not only consistent with NLDAS and CRU but also captured fine scale spatial structures that are missing in the global model simulations due to their coarse resolution. In addition, the annual cycle and intera-anual variability, particularly that of temperature, are reasonably well reproduced by the PRECIS. When comparing the PRECIS simulations with the driving GCMs, PRECIS is sensitive to the choice of the driving GCM, suggesting a careful selection of driving GCM based on the current climate performance for the use of future climate impact assessment. Quantitative understanding of the climate system and better estimation of the fresh water balance over the Middle Tennessee Elk watershed is a vital corner stone for a sustainable economic growth of the region over the coming decades.

  13. Seismological Structure of the 1.8Ga Trans-Hudson Orogen of North America and its affinity to present-day Tibet

    NASA Astrophysics Data System (ADS)

    Gilligan, A.; Bastow, I. D.; Darbyshire, F. A.

    2015-12-01

    How tectonic processes operated and changed through the Precambrian is debated: what was the nature and scale of orogenic events and were they different on the younger, hotter, more ductile Earth? The geology of northern Hudson Bay records the Paleoproterozoic collision between the Western Churchill and Superior plates: the 1.8Ga Trans-Hudson Orogeny (THO) and is thus an ideal study locale to address this issue. It has been suggested, primarily on the strength of traditional field geology, that the THO was comparable in scale and style to the present-day Himalayan-Karakoram-Tibet Orogen (HKTO). However, understanding of the deep crustal architecture of the THO, and how it compares to the evolving HKTO is presently lacking. Through joint inversion of teleseismic receiver functions and surface wave data, we obtain new Moho depth estimates and shear velocity models for the crust and upper mantle. Archean crust in the Rae, Hearne and Churchill domains is thin and structurally simple, with a sharp Moho; upper crustal wavespeed variations are readily attributed to post-formation events. However, the Paleoproterozoic Quebec-Baffin segment of the THO has a deeper Moho and more complex crustal structure. Our observations are strikingly similar to recent models, computed using the same methods, of the HKTO lithosphere, where deformation also extends >400km beyond the collision front. On the strength of Moho character, present-day crustal thickness, and metamorphic grade, we thus propose that southern Baffin experienced uplift of a similar magnitude and spatial extent to the Himalayas during the Paleoproterozoic Trans-Hudson Orogeny.

  14. Importance of the margin inheritance in the present-day crustal structure of the Longmen Shan range, Eastern border of the Tibetan plateau

    NASA Astrophysics Data System (ADS)

    Robert, A.; Pubellier, M.; de Sigoyer, J.; Billerot, A.; Vergne, J.

    2011-12-01

    The Longmen Shan range, located at the eastern border of the Tibetan plateau (Sichuan province, China), is characterized by one of the most important topographic gradient of the world whereas the present-day convergence recorded by GPS measurements is quite low (~3mm/yr). The mountain is also located above an abrupt 18km-high Moho step interpreted as the result of the confrontation of the thick Tibetan crust (~63km) against the resistant Yangtze craton (~45km). This peculiar crustal structure, as well as the thickening of the Tibetan crust in its eastern part, is often considerate as a result of the Cenozoic collision between India and Eurasia. This study highlights the importance of structural and thermal inheritance in the current crustal structure of this area. In fact, sedimentary records indicate that the Longmen Shan formed an abrupt passive margin from the beginning of the Paleozoic to the middle Triassic as a consequence of the opening of the Prototethys and the Paleotethys. The Longmen Shan correspond to a major paleogeographic boundary between the shallow water sediments of the Sichuan basin to the South-East and the deep sea mostly flysch-type sediments of the Songpan Garze terrane to the North West. Moreover, geochimical data indicate an important extension of the continental crust further west of the sedimentary transition. The oblique position of the Longmen Shan compared to the direction of opening is responsible of a trantensive passive margin structure as observed for the present-day vietnamian passive margin. This geometry is characterized by an abrupt decrease of the continental crust thickness associated with the sedimentary transition whereas the continent/ocean transition is located further away. We propose that the peculiar geometry of the old passive margin in the Longmen Shan is responsible for the important localization of the deformation during the 3 reactivations of the range (Triassic, Cretaceous and Late Tertiary).

  15. Present-day cosmic abundances. A comprehensive study of nearby early B-type stars and implications for stellar and Galactic evolution and interstellar dust models

    NASA Astrophysics Data System (ADS)

    Nieva, M.-F.; Przybilla, N.

    2012-03-01

    Context. Early B-type stars are ideal indicators for present-day cosmic abundances since they preserve their pristine abundances and typically do not migrate far beyond their birth environments over their short lifetimes, in contrast to older stars like the Sun. They are also unaffected by depletion onto dust grains, unlike the cold/warm interstellar medium (ISM) or H ii regions. Aims: A carefully selected sample of early B-type stars in OB associations and the field within the solar neighbourhood is studied comprehensively. Quantitative spectroscopy is used to characterise their atmospheric properties in a self-consistent way. Present-day abundances for the astrophysically most interesting chemical elements are derived in order to investigate whether a present-day cosmic abundance standard can be established. Methods: High-resolution and high-S/N FOCES, FEROS and ELODIE spectra of well-studied sharp-lined early B-type stars are analysed in non-LTE. Line-profile fits based on extensive model grids and an iterative analysis methodology are used to constrain stellar parameters and elemental abundances at high accuracy and precision. Atmospheric parameters are derived from the simultaneous establishment of independent indicators, from multiple ionization equilibria and the Stark-broadened hydrogen Balmer lines, and they are confirmed by reproduction of the stars' global spectral energy distributions. Results: Effective temperatures are constrained to 1-2% and surface gravities to less than 15% uncertainty, along with accurate rotational, micro- and macroturbulence velocities. Good agreement of the resulting spectroscopic parallaxes with those from the new reduction of the Hipparcos catalogue is obtained. Absolute values for abundances of He, C, N, O, Ne, Mg, Si and Fe are determined to better than 25% uncertainty. The synthetic spectra match the observations reliably over almost the entire visual spectral range. Three sample stars, γ Ori, o Per and θ1 Ori D, are

  16. Experimental evolution of an emerging plant virus in host genotypes that differ in their susceptibility to infection.

    PubMed

    Hillung, Julia; Cuevas, José M; Valverde, Sergi; Elena, Santiago F

    2014-09-01

    This study evaluates the extent to which genetic differences among host individuals from the same species condition the evolution of a plant RNA virus. We performed a threefold replicated evolution experiment in which Tobacco etch potyvirus isolate At17b (TEV-At17b), adapted to Arabidopsis thaliana ecotype Ler-0, was serially passaged in five genetically heterogeneous ecotypes of A. thaliana. After 15 passages we found that evolved viruses improved their fitness, showed higher infectivity and stronger virulence in their local host ecotypes. The genome of evolved lineages was sequenced and putative adaptive mutations identified. Host-driven convergent mutations have been identified. Evidences supported selection for increased translational efficiency. Next, we sought for the specificity of virus adaptation by infecting all five ecotypes with all 15 evolved virus populations. We found that some ecotypes were more permissive to infection than others, and that some evolved virus isolates were more specialist/generalist than others. The bipartite network linking ecotypes with evolved viruses was significantly nested but not modular, suggesting that hard-to-infect ecotypes were infected by generalist viruses whereas easy-to-infect ecotypes were infected by all viruses, as predicted by a gene-for-gene model of infection.

  17. Phenotypic differences on the outcome of the host-parasite relationship: behavior of mice of the CBi stock in natural and experimental infections.

    PubMed

    Vasconi, M D; Malfante, P; Bassi, A; Giudici, C; Revelli, S; Di Masso, R; Font, M T; Hinrichsen, L

    2008-05-01

    Investigation of defined animal models may help to elucidate the role of the host genetic background in the development and establishment of a parasitic infection. Four lines of mice obtained by disruptive selection for body conformation (CBi+, CBi-, CBi/C and CBi/L) and the unselected control line CBi were examined in their response to different parasites to assess whether these distinct genotypes showed differences in their resistance to natural and experimental parasitosis. Protozoans (Trichomonas muris and Spironucleus muris) and nemathelminths (Syphacia obvelata and Aspiculurus tetraptera) were found naturally parasitizing the mice's intestines. CBi/C and CBi were the only genotypes in which T. muris was found. CBi- was least resistant to S. muris. The helminth parasitic burden showed differences between sexes within genotypes (males had a higher burden than females) and among genotypes (CBi/L males had the lowest burden). CBi/L animals were also most resistant to experimental challenge with Heligmosomoides polygyrus and Trypanosoma cruzi. Since all the animals examined shared a common habitat throughout the study and were equally exposed to infection, the phenotypic differences in the natural enteroparasitism herein described evince genetic differences among lines in the host-parasite relationship. This interpretation is further supported by the differences in the response to the experimental challenge to H. polygyrus and T. cruzi. PMID:18304738

  18. UV-B absorbing compounds in present-day and fossil pollen, spores, cuticles, seed coats and wood: evaluation of a proxy for solar UV radiation.

    PubMed

    Rozema, J; Blokker, P; Mayoral Fuertes, M A; Broekman, R

    2009-09-01

    UV-B absorbing compounds (UACs) in present-day and fossil pollen, spores, cuticles, seed coats and wood have been evaluated as a proxy for past UV. This proxy may not only provide information on variation of stratospheric ozone and solar UV in the period preceding and during the Antarctic ozone hole (1974-present day), but also on the development and variation of the stratospheric ozone layer and solar surface UV during the evolution of life on Earth. Sporopollenin and cutin are highly resistant biopolymers, preserving well in the geological record and contain the phenolic acids p-coumaric (pCA) and ferulic acid (FA). pCA and FA represent a good perspective for a plant-based proxy for past surface UV radiation since they are induced by solar UV-B via the phenylpropanoid pathway (PPP). UV-B absorption by these monomers in the wall of pollen and spores and in cuticles may prevent damage to the cellular metabolism. Increased pCA and FA in pollen of Vicia faba exposed to enhanced UV-B was found in greenhouse experiments. Further correlative evidence comes from UV-absorbing compounds in spores from 1960-2000 comparing exposure of land plants (Lycopodium species) to solar UV before and during ozone depletion and comparing plants from Antarctica (severe ozone depletion), Arctic, and other latitudes with less or negligible ozone depletion. Wood-derived compounds guaiacyl (G), syringyl (S), and p-hydroxyphenyl (P) are produced via the PPP. The proportions of P, G, and S in the lignin differ between various plant groups (e.g. dicotyledons/monocotyledons, gymnosperms/angiosperms). It is hypothesized that this lignin composition and derived physiological and physical properties of lignin (such as tree-ring wood density) has potential as a proxy for palaeo-UV climate. However validation by exposure of trees to enhanced UV is lacking. pCA and FA also form part of cutin polymers and are found in extant and fossil Ginkgo leaf cuticles as shown by thermally-assisted hydrolysis and

  19. Differential Responses of Calcifying and Non-Calcifying Epibionts of a Brown Macroalga to Present-Day and Future Upwelling pCO2

    PubMed Central

    Saderne, Vincent; Wahl, Martin

    2013-01-01

    Seaweeds are key species of the Baltic Sea benthic ecosystems. They are the substratum of numerous fouling epibionts like bryozoans and tubeworms. Several of these epibionts bear calcified structures and could be impacted by the high pCO2 events of the late summer upwellings in the Baltic nearshores. Those events are expected to increase in strength and duration with global change and ocean acidification. If calcifying epibionts are impacted by transient acidification as driven by upwelling events, their increasing prevalence could cause a shift of the fouling communities toward fleshy species. The aim of the present study was to test the sensitivity of selected seaweed macrofoulers to transient elevation of pCO2 in their natural microenvironment, i.e. the boundary layer covering the thallus surface of brown seaweeds. Fragments of the macroalga Fucus serratus bearing an epibiotic community composed of the calcifiers Spirorbis spirorbis (Annelida) and Electra pilosa (Bryozoa) and the non-calcifier Alcyonidium hirsutum (Bryozoa) were maintained for 30 days under three pCO2 conditions: natural 460±59 µatm, present-day upwelling1193±166 µatm and future upwelling 3150±446 µatm. Only the highest pCO2 caused a significant reduction of growth rates and settlement of S. spirorbis individuals. Additionally, S. spirorbis settled juveniles exhibited enhanced calcification of 40% during daylight hours compared to dark hours, possibly reflecting a day-night alternation of an acidification-modulating effect by algal photosynthesis as opposed to an acidification-enhancing effect of algal respiration. E. pilosa colonies showed significantly increased growth rates at intermediate pCO2 (1193 µatm) but no response to higher pCO2. No effect of acidification on A. hirsutum colonies growth rates was observed. The results suggest a remarkable resistance of the algal macro-epibionts to levels of acidification occurring at present day upwellings in the Baltic. Only extreme future

  20. Present-Day Strain Transfer Across the Yakutat Collision in SW Yukon - SE Alaska: The Death of the Southern Denali Fault?

    NASA Astrophysics Data System (ADS)

    Marechal, A.; Mazzotti, S.; Ritz, J. F.; Ferry, M. A.; Freymueller, J. T.

    2014-12-01

    In SW Yukon-SE Alaska, the present-day Pacific-North America relative motion is highly oblique to the main plate boundary, resulting in strong strain-partitioning tectonics that link the Aleutian subduction to the west to Queen Charlotte transform to the south. This transition region is also the site of present-day orogeny and accretion of the Yakutat Terrane to the Northern Cordillera. Multiple datasets (GPS, geomorphology, seismicity) are integrated to characterize and quantify strain patterns, with particular emphasis on strain partitioning between strike-slip and shortening deformation. New GPS data straddling the main faults (Denali, Totschunda, Fairweather) indicate that, south of the collision corner, 95% of the Pacific-North America strike-slip motion is accommodated on the plate-boundary Fairweather Fault, leaving near-zero motion on the Denali Fault only ~100 km inboard. In contrast, the fault-perpendicular component is strongly distributed between shortening offshore, in the orogen, and inland outward motion. In the region of highest convergence obliquity, GPS data show a diffuse indentor-like deformation, with strong along-strike variations of the main fault slip rates. Preliminary results of a regional geomorphology study give further information about the Denali Fault, where previous data suggest a velocity decrease from 8 mm/yr (Matmon et al.,2006) to 4 mm/yr (Seitz et al., 2010). A high resolution DEM processed from Pleiades satellite imagery highlights a significant vertical component on the Denali Fault and very little to no strike-slip movement in its southern part. Metric-scale displacements are measured along the "inactive" part of the fault showing recent vertical deformation since the Last Glacial Maximum (~20 kyrs ago). In contrast, significant dextral offsets on post-LGM structures are measured on the southern Totschunda Fault. Ongoing datation of geomorphological markers (Be10, OSL) will give us new slip-rate estimates along the southern

  1. Human responses to eruptions of Etna (Sicily) during the late-Pre-Industrial Era and their implications for present-day disaster planning

    NASA Astrophysics Data System (ADS)

    Chester, David K.; Duncan, Angus M.; Sangster, Heather

    2012-05-01

    This paper summarises: the characteristics of eruptions that occurred between 1792/3 and 1923; the ways in which human responses evolved during the period and the lessons this history holds for the management of present-day volcanic and volcano-related disasters. People responded to eruptions at three levels: as members of a family and extended family; through the mutual support of a village or larger settlement and as citizens of the State. During the study period and with the exception of limited financial aid and preservation of law and order, the State was a minor player in responding to eruptions. Families and extended families provided shelter, accommodation and often alternative agricultural employment; whilst supportive villages communities displayed a well developed tendency to learn from experience (e.g. innovating techniques to bring land back into cultivation and avoiding the risks of phreatic activity as lava encountered water and saturated ground) and providing labour to enable household chattels and agricultural crops to be salvaged from land threatened with lava incursion. Eruptions were widely believed to be 'Acts of God', with divine punishment frequently being invoked as a primary cause of human suffering. Elaborate rituals of propitiation were performed to appease a supposed angry God, but this world-view did not produce a fatalistic attitude amongst the population preventing people from coping with disasters in a generally effective manner. Despite present day emergencies being handled by the State and its agencies, some features of nineteenth century responses remain in evidence, including salvaging all that may be easily removed from a building and/or agricultural holding, and explanations of disaster which are theistic in character. Lessons from eruptions that occurred between 1792/3 to 1923 are that the former should be encouraged, whilst the latter does not prevent people acting to preserve life and property or obeying the authorities

  2. UV-B absorbing compounds in present-day and fossil pollen, spores, cuticles, seed coats and wood: evaluation of a proxy for solar UV radiation.

    PubMed

    Rozema, J; Blokker, P; Mayoral Fuertes, M A; Broekman, R

    2009-09-01

    UV-B absorbing compounds (UACs) in present-day and fossil pollen, spores, cuticles, seed coats and wood have been evaluated as a proxy for past UV. This proxy may not only provide information on variation of stratospheric ozone and solar UV in the period preceding and during the Antarctic ozone hole (1974-present day), but also on the development and variation of the stratospheric ozone layer and solar surface UV during the evolution of life on Earth. Sporopollenin and cutin are highly resistant biopolymers, preserving well in the geological record and contain the phenolic acids p-coumaric (pCA) and ferulic acid (FA). pCA and FA represent a good perspective for a plant-based proxy for past surface UV radiation since they are induced by solar UV-B via the phenylpropanoid pathway (PPP). UV-B absorption by these monomers in the wall of pollen and spores and in cuticles may prevent damage to the cellular metabolism. Increased pCA and FA in pollen of Vicia faba exposed to enhanced UV-B was found in greenhouse experiments. Further correlative evidence comes from UV-absorbing compounds in spores from 1960-2000 comparing exposure of land plants (Lycopodium species) to solar UV before and during ozone depletion and comparing plants from Antarctica (severe ozone depletion), Arctic, and other latitudes with less or negligible ozone depletion. Wood-derived compounds guaiacyl (G), syringyl (S), and p-hydroxyphenyl (P) are produced via the PPP. The proportions of P, G, and S in the lignin differ between various plant groups (e.g. dicotyledons/monocotyledons, gymnosperms/angiosperms). It is hypothesized that this lignin composition and derived physiological and physical properties of lignin (such as tree-ring wood density) has potential as a proxy for palaeo-UV climate. However validation by exposure of trees to enhanced UV is lacking. pCA and FA also form part of cutin polymers and are found in extant and fossil Ginkgo leaf cuticles as shown by thermally-assisted hydrolysis and

  3. Hazard responses in the pre-industrial era: vulnerability and resilience of traditional societies to volcanic disasters and the implications for present-day disaster planning

    NASA Astrophysics Data System (ADS)

    Sangster, Heather

    2014-05-01

    A major research frontier in the study of natural hazard research involves unravelling the ways in which societies have reacted historically to disasters, and how such responses influence current policies of disaster reduction. For societies it is common to classify responses to natural hazards into: pre-industrial (folk); industrial; and post-industrial (comprehensive) responses. Pre-industrial societies are characterised by: a pre-dominantly rural location; an agricultural economic focus; artisan handicrafts rather than industrial production, parochialism, with people rarely travelling outside their local area and being little affected by external events and a feudal or semi-feudal social structure. In the past, hazard assessment focused on the physical processes that produced extreme and potentially damaging occurrences, however from the middle of the twenty-first century research into natural hazards has been cast within a framework defined by the polarities (or opposites) of vulnerability and resilience, subject to a blend of unique environmental, social, economic and cultural forces in hazardous areas, that either increase or decrease the impact of extreme events on a given society. In the past decade research of this type has been facilitated by a 'revolution' of source materials across a range of languages and in a variety of electronic formats (e.g. official archives; major contemporary and near-contemporary publications - often available as reprints; national and international newspapers of record; newsreel-films; and, photographs) and in the introduction of more reliable translation software (e.g. Systrans) that provides far more scope to the researcher in the study of natural hazards than was the case even a few years ago. Knowledge of hazard responses in the pre-industrial era is, not only important in its own right because it reveals indigenous strategies of coping, but also informs present-day disaster planners about how people have reacted to past

  4. Differential responses of calcifying and non-calcifying epibionts of a brown macroalga to present-day and future upwelling pCO2.

    PubMed

    Saderne, Vincent; Wahl, Martin

    2013-01-01

    Seaweeds are key species of the Baltic Sea benthic ecosystems. They are the substratum of numerous fouling epibionts like bryozoans and tubeworms. Several of these epibionts bear calcified structures and could be impacted by the high pCO2 events of the late summer upwellings in the Baltic nearshores. Those events are expected to increase in strength and duration with global change and ocean acidification. If calcifying epibionts are impacted by transient acidification as driven by upwelling events, their increasing prevalence could cause a shift of the fouling communities toward fleshy species. The aim of the present study was to test the sensitivity of selected seaweed macrofoulers to transient elevation of pCO2 in their natural microenvironment, i.e. the boundary layer covering the thallus surface of brown seaweeds. Fragments of the macroalga Fucus serratus bearing an epibiotic community composed of the calcifiers Spirorbis spirorbis (Annelida) and Electra pilosa (Bryozoa) and the non-calcifier Alcyonidium hirsutum (Bryozoa) were maintained for 30 days under three pCO2 conditions: natural 460 ± 59 µatm, present-day upwelling1193 ± 166 µatm and future upwelling 3150 ± 446 µatm. Only the highest pCO2 caused a significant reduction of growth rates and settlement of S. spirorbis individuals. Additionally, S. spirorbis settled juveniles exhibited enhanced calcification of 40% during daylight hours compared to dark hours, possibly reflecting a day-night alternation of an acidification-modulating effect by algal photosynthesis as opposed to an acidification-enhancing effect of algal respiration. E. pilosa colonies showed significantly increased growth rates at intermediate pCO2 (1193 µatm) but no response to higher pCO2. No effect of acidification on A. hirsutum colonies growth rates was observed. The results suggest a remarkable resistance of the algal macro-epibionts to levels of acidification occurring at present day upwellings in the Baltic. Only extreme

  5. Experimental control of Biomphalaria pfeifferi, the intermediate host of Schistosoma mansoni, by the ampullariid snail Lanistes varicus.

    PubMed

    Anto, F; Bosompem, K; Kpikpi, J; Adjuik, M; Edoh, D

    2005-03-01

    The biological control of the snail hosts of the trematodes that cause human schistosomiasis appears to be a promising method for achieving sustainable reductions in the transmission of the parasites. The possibility of using the Ghanaian strain of an ampullariid snail, Lanistes varicus, for the biological control of the main snail host of Schistosoma mansoni , Biomphalaria pfeifferi, has now been investigated in laboratory-based experiments. Adult and 2-week-old L. varicus were found to feed voraciously on the egg masses and juveniles of B. pfeifferi (from the Tono irrigation canals in northern Ghana). When single L. varicus were exposed to 20-200 egg masses, they consumed all of the masses over 24 h (if adult) or about 50% of them over 4 days (if 2-week-old juveniles). The effect of the secretions of the ampullariid on the reproduction, growth and mortality of B. pfeifferi was also investigated, by maintaining the two snail species in the same aquarium but separated by nylon netting. The presence of L. varicus in the same aquarium reduced the number of egg masses produced by each B. pfeifferi, although, curiously, the presence of a single L. varicus in the aquarium appeared to have more of an impact, on the egg-mass deposition by 20 B. pfeifferi, than the presence of five or more of the ampullariids. It appears that, under laboratory conditions at least, the Ghanaian stain of L. varicus has the potential to limit populations of B. pfeifferi.

  6. Effects of submarine groundwater discharge on the present-day extent of relict submarine permafrost and gas hydrate stability on the Beaufort Sea continental shelf

    NASA Astrophysics Data System (ADS)

    Frederick, J. M.; Buffett, B. A.

    2015-03-01

    We investigate the role of submarine groundwater discharge on the offshore temperature and salinity field and its effect on the present-day extent of submarine permafrost and gas hydrate stability on the North American Beaufort Shelf with a two-dimensional numerical model based on the finite volume method. This study finds that submarine groundwater discharge can play a large role in submarine permafrost evolution and gas hydrate stability, suggesting that local hydrology may control the evolution of submarine permafrost as strongly as does sea level or paleoclimatic conditions. Submarine permafrost evolution shows transient behavior over potentially long time scales (e.g., several glacial cycles) before a balance of density- and pressure-driven flows is established with the permeability variations imposed by the overlying permafrost layer. The "detectable" offshore permafrost extent is related to the quasi-stationary location of the saltwater-freshwater transition. Larger values of submarine groundwater discharge allow permafrost to extend farther offshore because fresh pore water preserves relict ice. Therefore, differences in the permafrost extent at locations that share similar paleoclimatic history may be explained in part by differences in the local hydrology. Gas hydrate stability on the North American Beaufort Shelf may be more widespread than currently thought because low-ice saturation, highly degraded submarine permafrost likely exists beyond the boundary detectable by common geophysical methods.

  7. Sulfur Dioxide and the Production of Sulfuric Acid on Present-Day and Early Mars: Implications for the Lack of Detected Carbonates on the Surface

    NASA Technical Reports Server (NTRS)

    Levine, Joel S.; Summers, Michael E.

    2008-01-01

    In the early history of Mars, volcanic activity associated with the formation of the Tharsis ridge produced a very large amount of atmospheric SO2--on the order of a bar of atmospheric SO2. In the present-day atmosphere of Mars, the lifetime of SO2 is relatively short with a lifetime of less than a day. The short lifetime of SO2 in the present Mars atmosphere makes the production of significant levels of H2SO4 very difficult since the SO2 may be destroyed by various chemical and photochemical processes before the SO2 can be converted to H2SO4. However, photochemical calculations performed and described here, indicate that enhanced atmospheric levels of CO2 in the early atmosphere of Mars resulted in a significantly enhanced atmospheric lifetime for SO2 up to several years. With a significantly enhanced atmospheric lifetime, SO2 could readily form large amounts of H2SO4, which precipitated out of the atmosphere in the form of droplets. The precipitated H2SO4 then reacted with potential surface carbonates, destroying the carbonates and resulting in the abundant and widespread distribution of sulfates on the surface of Mars as detected by recent Mars missions.

  8. Laser-assisted cavity preparation and adhesion to erbium-lased tooth structure: part 2. present-day adhesion to erbium-lased tooth structure in permanent teeth.

    PubMed

    De Moor, Roeland Jozef Gentil; Delme, Katleen Ilse Maria

    2010-04-01

    With the introduction of the Er:YAG laser, it has become possible to remove enamel and dentin more effectively and efficiently than with other lasers. Thermal damage is reduced, especially in conjunction with water spray. Since FDA (Federal Drug Administration) approval of the Er:YAG laser in 1997--for caries removal, cavity preparation and conditioning of tooth substance - there have been many reports on the use of this technique in combination with composite resins. Moreover, cavity pretreatment with Er:YAG laser (laser etching) has been proposed as an alternative to acid etching of enamel and dentin. Reports evaluating the adhesion of glass-ionomer cements to Er:YAG-lased tooth substance are scarce. This article reviews the literature regarding adhesion and sealing efficacy using different (pre)treatment protocols in association with Er:YAG laser preparation. Recent research has shown that lasing of enamel and dentin may result in surface and subsurface alterations that have negative effects on both adhesion and seal. It is concluded that at present, it is advisable to respect the conventional pretreatment procedures as needed for the respective adhesive materials. Although the majority of present day reports show that microleakage and bond strength are negatively influenced by laser (pre)treatment (compared with conventional preparation), there is ongoing discussion of how adhesion is best achieved on Er:YAG-lased surfaces.

  9. Investigations of potential microbial methanogenic and carbon monoxide utilization pathways in ultra-basic reducing springs associated with present-day continental serpentinization: the Tablelands, NL, CAN

    PubMed Central

    Morrill, Penny L.; Brazelton, William J.; Kohl, Lukas; Rietze, Amanda; Miles, Sarah M.; Kavanagh, Heidi; Schrenk, Matthew O.; Ziegler, Susan E.; Lang, Susan Q.

    2014-01-01

    Ultra-basic reducing springs at continental sites of serpentinization act as portals into the biogeochemistry of a subsurface environment with H2 and CH4 present. Very little, however, is known about the carbon substrate utilization, energy sources, and metabolic pathways of the microorganisms that live in this ultra-basic environment. The potential for microbial methanogenesis with bicarbonate, formate, acetate, and propionate precursors and carbon monoxide (CO) utilization pathways were tested in laboratory experiments by adding substrates to water and sediment from the Tablelands, NL, CAD, a site of present-day continental serpentinization. Microbial methanogenesis was not observed after bicarbonate, formate, acetate, or propionate addition. CO was consumed in the live experiments but not in the killed controls and the residual CO in the live experiments became enriched in 13C. The average isotopic enrichment factor resulting from this microbial utilization of CO was estimated to be 11.2 ± 0.2‰. Phospholipid fatty acid concentrations and δ13C values suggest limited incorporation of carbon from CO into microbial lipids. This indicates that in our experiments, CO was used primarily as an energy source, but not for biomass growth. Environmental DNA sequencing of spring fluids collected at the same time as the addition experiments yielded a large proportion of Hydrogenophaga-related sequences, which is consistent with previous metagenomic data indicating the potential for these taxa to utilize CO. PMID:25431571

  10. Human reponses to historical eruptions of Etna (Sicily) from 1600 to present and their implications for present-day disaster planning

    NASA Astrophysics Data System (ADS)

    Sangster, H.; Chester, D. K.; Duncan, A. M.

    2012-04-01

    Mount Etna in northeastern Sicily (Italy) rises to over 3000 m, covers an area of ca.1750 km2 and is the most active volcano in Europe. Observations of Etna by literate observers stretch back to the classical era and one of the earliest references to an eruption of Etna was by Pindar in his Pythian Odes, to the event of ca. 474-479 B.C. The history of its activity has been reconstructed by scholars up to the present day and records of eruptions are reasonably complete from the early fifteenth century, reliable from 1669, and document the threats and destruction to human settlements and livelihoods. Effusive and explosive activity has occurred continually throughout the historical period and eruptions of Mount Etna have presented numerous eruption styles, from persistent central crater activity, to periodic flank eruptions. From 1600 to 1669 the activity of Etna was characterised by a high volumetric output of lava with a mean eruption rate of 1.19 m3s-1, this was followed by a pause from flank eruptions and the re-establishment of significant activity from the middle of the eighteenth century. After 1750 the output of lava by flank eruptions was lower than in the previous century, with the mean eruption rate falling to 0.18 m3s-1. This paper summarises: the characteristics of the eruptions that occurred between the period of 1600 to present; the particularities of the societal responses over time and the role of the authorities; and, the important lessons this history holds for the management of present-day civil defence planning in the region. People responded to the eruptions at three levels: as members of a family and extended family; as members of a community and, as citizens of the State. The State, however, was a minor player in responding to these eruptions until the early nineteenth century as the State then became more involved in each successive eruption as the responses moved to a more industrial nature rather than pre-industrial. Today emergencies are

  11. [Embolism of the aortic bifurcation and major arteries of limbs: lessons of the past and present-day trends in solving the problem concerned].

    PubMed

    Mel'nikov, M V; Barsukov, A E; Apresian, A Iu; Isaulov, O V

    2013-01-01

    The works deals with a retrospective analysis of the medical records of the Clinic of General Surgery of the North-West State Medical University named after I.I. Mechnikov on treatment of patients presenting with embolism of the aorta and major arteries over 40 years. All in all, over the period from 1971 to 2010 a total of 3,110 patients with embolism of the aorta and major arteries underwent consultations and were operated on. To the present-day trends in surgery of embologenic arterial obstruction one should first of all refer a decrease in the number of patients with embolism of the aorta and major arteries of the limbs, which may be related to achievement in modern cardiology and cardiosurgery in treatment of patients with cardiovascular diseases - potential sources of arterial embolism. Besides, there occurred considerable changes in the structure of embologenic diseases, in favour of an increased number of people suffering from CAD, which to e certain degree modified the incidence of lesions of various vascular basins. Thus, the number of embolisms of proximal portions of the vascular bed decreased considerably. This is largely related to a decrease in the number of patients presenting with decompensated ischaemia of extremities. 86.9% of patients were subjected to emergency operations. An increased number of people with atherosclerosis of peripheral arteries required widening of indications for performing reconstructive-and-plastic operative interventions. Experience of the Clinic shows that a timely performed revascularizing operation, including a reconstructive on, application of modern methods of prevention of ischaemic syndrome, carrying out comprehensive rehabilitation measures in the postoperative period made it possible to considerably improve the immediate results of treatment. While during the first 20 years a total lethality rate amounted to 18.8% with the postoperative one equalling 17.1%, these parameters over the past 10 years were 8.8% and 6

  12. Evaluation of Preindustrial to Present-day Black Carbon and its Albedo Forcing from Atmospheric Chemistry and Climate Model Intercomparison Project (ACCMIP)

    SciTech Connect

    Lee, Y. H.; Lamarque, J.-F.; Flanner, M. G.; Jiao, C.; Shindell, Drew; Berntsen, T.; Bisiauxs, M.; Cao, J.; Collins, W. J.; Curran, M.; Edwards, R.; Faluvegi, G.; Ghan, Steven J.; Horowitz, L.; McConnell, J.R.; Ming, J.; Myhre, G.; Nagashima, T.; Naik, Vaishali; Rumbold, S.; Skeie, R. B.; Sudo, K.; Takemura, T.; Thevenon, F.; Xu, B.; Yoon, Jin-Ho

    2013-03-05

    As a part of the Atmospheric Chemistry and Climate Model Intercomparison Project (ACCMIP), we evaluate the historical black carbon (BC) aerosols simulated by 8 ACCMIP models against the observations including 12 ice core records, a long-term surface mass concentrations and recent Arctic BC snowpack measurements. We also estimate BC albedo forcing by performing additional simulations using the NCAR Community Land and Sea-Ice model 4 with prescribed meteorology from 1996-2000, which includes the SNICAR BC-snow model. We evaluated the vertical profile of BC snow concentrations from these offline simulations to using recent BC snowpack measurements. Despite using the same BC emissions, global BC burden differs by approximately a factor of 3 among models due to the differences in aerosol removal parameterizations and simulated meteorology among models; 34 Gg to 103 Gg in 1850 and 82 Gg to 315 Gg in 2000. However,models agree well on 2.5~3 times increase in the global BC burden from preindustrial to present-day, which matches with the 2.5 times increase in BC emissions. We find a large model diversity at both NH and SH high latitude regions for BC burden and at SH high latitude regions for deposition fluxes. The ACCMIP simulations match the observed BC mass concentrations well in Europe and North America except at Jungfrauch and Ispra. However, the models fail to capture the Arctic BC seasonality due tosevere underestimations during winter and spring. Compared to recent snowpack measurements, the simulated vertically resolved BC snow concentrations are, on average, within a factor of 2-3 of observations except for Greenland and Arctic Ocean. However, model and observation differ widely due to missing interannual variations in emissions and possibly due to the choice of the prescribed meteorology period (i.e., 1996-2000).

  13. Geodetic measurements reveal similarities between post–Last Glacial Maximum and present-day mass loss from the Greenland ice sheet

    PubMed Central

    Khan, Shfaqat A.; Sasgen, Ingo; Bevis, Michael; van Dam, Tonie; Bamber, Jonathan L.; Wahr, John; Willis, Michael; Kjær, Kurt H.; Wouters, Bert; Helm, Veit; Csatho, Beata; Fleming, Kevin; Bjørk, Anders A.; Aschwanden, Andy; Knudsen, Per; Munneke, Peter Kuipers

    2016-01-01

    Accurate quantification of the millennial-scale mass balance of the Greenland ice sheet (GrIS) and its contribution to global sea-level rise remain challenging because of sparse in situ observations in key regions. Glacial isostatic adjustment (GIA) is the ongoing response of the solid Earth to ice and ocean load changes occurring since the Last Glacial Maximum (LGM; ~21 thousand years ago) and may be used to constrain the GrIS deglaciation history. We use data from the Greenland Global Positioning System network to directly measure GIA and estimate basin-wide mass changes since the LGM. Unpredicted, large GIA uplift rates of +12 mm/year are found in southeast Greenland. These rates are due to low upper mantle viscosity in the region, from when Greenland passed over the Iceland hot spot about 40 million years ago. This region of concentrated soft rheology has a profound influence on reconstructing the deglaciation history of Greenland. We reevaluate the evolution of the GrIS since LGM and obtain a loss of 1.5-m sea-level equivalent from the northwest and southeast. These same sectors are dominating modern mass loss. We suggest that the present destabilization of these marine-based sectors may increase sea level for centuries to come. Our new deglaciation history and GIA uplift estimates suggest that studies that use the Gravity Recovery and Climate Experiment satellite mission to infer present-day changes in the GrIS may have erroneously corrected for GIA and underestimated the mass loss by about 20 gigatons/year. PMID:27679819

  14. A new method to estimate air-quality levels using a synoptic-regression approach. Part I: Present-day O 3 and PM 10 analysis

    NASA Astrophysics Data System (ADS)

    Demuzere, Matthias; van Lipzig, Nicole P. M.

    2010-03-01

    In order to make projections for future air-quality levels, a robust methodology is needed that succeeds in reconstructing present-day air-quality levels. At present, climate projections for meteorological variables are available from Atmospheric-Ocean Coupled Global Climate Models (AOGCMs) but the temporal and spatial resolution is insufficient for air-quality assessment. Therefore, a variety of methods are tested in this paper in their ability to hindcast maximum 8 hourly levels of O 3 and daily mean PM 10 from observed meteorological data. The methods are based on a multiple linear regression technique combined with the automated Lamb weather classification. Moreover, we studied whether the above-mentioned multiple regression analysis still holds when driven by operational ECMWF (European Center for Medium-Range Weather Forecast) meteorological data. The main results show that a weather type classification prior to the regression analysis is superior to a simple linear regression approach. In contrast to PM 10 downscaling, seasonal characteristics should be taken into account during the downscaling of O 3 time series. Apart from a lower explained variance due to intrinsic limitations of the regression approach itself, a lower variability of the meteorological predictors (resolution effect) and model deficiencies, this synoptic-regression-based tool is generally able to reproduce the relevant statistical properties of the observed O 3 distributions important in terms of European air quality Directives and air quality mitigation strategies. For PM 10, the situation is different as the approach using only meteorology data was found to be insufficient to explain the observed PM 10 variability using the meteorological variables considered in this study.

  15. Estimating present day extreme water level exceedance probabilities around the coastline of Australia: tides, extra-tropical storm surges and mean sea level

    NASA Astrophysics Data System (ADS)

    Haigh, Ivan D.; Wijeratne, E. M. S.; MacPherson, Leigh R.; Pattiaratchi, Charitha B.; Mason, Matthew S.; Crompton, Ryan P.; George, Steve

    2014-01-01

    The occurrence of extreme water levels along low-lying, highly populated and/or developed coastlines can lead to considerable loss of life and billions of dollars of damage to coastal infrastructure. Therefore it is vitally important that the exceedance probabilities of extreme water levels are accurately evaluated to inform risk-based flood management, engineering and future land-use planning. This ensures the risk of catastrophic structural failures due to under-design or expensive wastes due to over-design are minimised. This paper estimates for the first time present day extreme water level exceedence probabilities around the whole coastline of Australia. A high-resolution depth averaged hydrodynamic model has been configured for the Australian continental shelf region and has been forced with tidal levels from a global tidal model and meteorological fields from a global reanalysis to generate a 61-year hindcast of water levels. Output from this model has been successfully validated against measurements from 30 tide gauge sites. At each numeric coastal grid point, extreme value distributions have been fitted to the derived time series of annual maxima and the several largest water levels each year to estimate exceedence probabilities. This provides a reliable estimate of water level probabilities around southern Australia; a region mainly impacted by extra-tropical cyclones. However, as the meteorological forcing used only weakly includes the effects of tropical cyclones, extreme water level probabilities are underestimated around the western, northern and north-eastern Australian coastline. In a companion paper we build on the work presented here and more accurately include tropical cyclone-induced surges in the estimation of extreme water level. The multi-decadal hindcast generated here has been used primarily to estimate extreme water level exceedance probabilities but could be used more widely in the future for a variety of other research and practical

  16. Interactive effects of ocean acidification and warming on coral reef associated epilithic algal communities under past, present-day and future ocean conditions

    NASA Astrophysics Data System (ADS)

    Vogel, N.; Cantin, N. E.; Strahl, J.; Kaniewska, P.; Bay, L.; Wild, C.; Uthicke, S.

    2016-06-01

    Epilithic algal communities play critical ecological roles on coral reefs, but their response to individual and interactive effects of ocean warming (OW) and ocean acidification (OA) is still largely unknown. We investigated growth, photosynthesis and calcification of early epilithic algal community assemblages exposed for 6 months to four temperature profiles (-1.1, ±0.0, +0.9, +1.6 °C) that were crossed with four carbon dioxide partial pressure (pCO2) levels (360, 440, 650, 940 µatm), under flow-through conditions and natural light regimes. Additionally, we compared the cover of heavily calcified crustose coralline algae (CCA) and lightly calcified red algae of the genus Peyssonnelia among treatments. Increase in cover of epilithic communities showed optima under moderately elevated temperatures and present pCO2, while cover strongly decreased under high temperatures and high-pCO2 conditions, particularly due to decreasing cover of CCA. Similarly, community calcification rates were strongly decreased at high pCO2 under both measured temperatures. While final cover of CCA decreased under high temperature and pCO2 (additive negative effects), cover of Peyssonnelia spp. increased at high compared to annual average and moderately elevated temperatures. Thus, cover of Peyssonnelia spp. increased in treatment combinations with less CCA, which was supported by a significant negative correlation between organism groups. The different susceptibility to stressors most likely derived from a different calcification intensity and/or mineral. Notably, growth of the epilithic communities and final cover of CCA were strongly decreased under reduced-pCO2 conditions compared to the present. Thus, CCA may have acclimatized from past to present-day pCO2 conditions, and changes in carbonate chemistry, regardless in which direction, negatively affect them. However, if epilithic organisms cannot further acclimatize to OW and OA, the interacting effects of both factors may change

  17. Paleontological records indicate the occurrence of open woodlands in a dry inland climate at the present-day Arctic coast in western Beringia during the Last Interglacial

    NASA Astrophysics Data System (ADS)

    Kienast, Frank; Wetterich, Sebastian; Kuzmina, Svetlana; Schirrmeister, Lutz; Andreev, Andrei A.; Tarasov, Pavel; Nazarova, Larisa; Kossler, Annette; Frolova, Larisa; Kunitsky, Viktor V.

    2011-08-01

    Permafrost records, accessible at outcrops along the coast of Oyogos Yar at the Dmitry Laptev Strait, NE-Siberia, provide unique insights into the environmental history of Western Beringia during the Last Interglacial. The remains of terrestrial and freshwater organisms, including plants, coleopterans, chironomids, cladocerans, ostracods and molluscs, have been preserved in the frozen deposits of a shallow paleo-lake and indicate a boreal climate at the present-day arctic mainland coast during the Last Interglacial. Terrestrial beetle and plant remains suggest the former existence of open forest-tundra with larch ( Larix dahurica), tree alder ( Alnus incana), birch and alder shrubs ( Duschekia fruticosa, Betula fruticosa, Betula divaricata, Betula nana), interspersed with patches of steppe and meadows. Consequently, the tree line was shifted to at least 270 km north of its current position. Aquatic organisms, such as chironomids, cladocerans, ostracods, molluscs and hydrophytes, indicate the formation of a shallow lake as the result of thermokarst processes. Steppe plants and beetles suggest low net precipitation. Littoral pioneer plants and chironomids indicate intense lake level fluctuations due to high evaporation. Many of the organisms are thermophilous, indicating a mean air temperature of the warmest month that was greater than 13 °C, which is above the minimum requirements for tree growth. These temperatures are in contrast to the modern values of less than 4 °C in the study area. The terrestrial and freshwater organism remains were found at a coastal exposure that was only 3.5 m above sea level and in a position where they should have been under sea during the Last Interglacial when the global sea level was 6-10 m higher than the current levels. The results suggest that during the last warm stage, the site was inland, and its modern coastal situation is the result of tectonic subsidence.

  18. Geodetic measurements reveal similarities between post–Last Glacial Maximum and present-day mass loss from the Greenland ice sheet

    PubMed Central

    Khan, Shfaqat A.; Sasgen, Ingo; Bevis, Michael; van Dam, Tonie; Bamber, Jonathan L.; Wahr, John; Willis, Michael; Kjær, Kurt H.; Wouters, Bert; Helm, Veit; Csatho, Beata; Fleming, Kevin; Bjørk, Anders A.; Aschwanden, Andy; Knudsen, Per; Munneke, Peter Kuipers

    2016-01-01

    Accurate quantification of the millennial-scale mass balance of the Greenland ice sheet (GrIS) and its contribution to global sea-level rise remain challenging because of sparse in situ observations in key regions. Glacial isostatic adjustment (GIA) is the ongoing response of the solid Earth to ice and ocean load changes occurring since the Last Glacial Maximum (LGM; ~21 thousand years ago) and may be used to constrain the GrIS deglaciation history. We use data from the Greenland Global Positioning System network to directly measure GIA and estimate basin-wide mass changes since the LGM. Unpredicted, large GIA uplift rates of +12 mm/year are found in southeast Greenland. These rates are due to low upper mantle viscosity in the region, from when Greenland passed over the Iceland hot spot about 40 million years ago. This region of concentrated soft rheology has a profound influence on reconstructing the deglaciation history of Greenland. We reevaluate the evolution of the GrIS since LGM and obtain a loss of 1.5-m sea-level equivalent from the northwest and southeast. These same sectors are dominating modern mass loss. We suggest that the present destabilization of these marine-based sectors may increase sea level for centuries to come. Our new deglaciation history and GIA uplift estimates suggest that studies that use the Gravity Recovery and Climate Experiment satellite mission to infer present-day changes in the GrIS may have erroneously corrected for GIA and underestimated the mass loss by about 20 gigatons/year.

  19. Relationship Between Pneumocystis carinii Burden and the Degree of Host Immunosuppression in an Airborne Transmission Experimental Model.

    PubMed

    Khalife, Sara; Chabé, Magali; Gantois, Nausicaa; Audebert, Christophe; Pottier, Muriel; Hlais, Sani; Pinçon, Claire; Chassat, Thierry; Pierrot, Christine; Khalife, Jamal; Aliouat-Denis, Cécile-Marie; Aliouat, El Moukhtar

    2016-05-01

    To quantitatively assess the risk of contamination by Pneumocystis depending on the degree of immunosuppression (ID) of the exposed rat hosts, we developed an animal model, where rats went through different doses of dexamethasone. Then, natural and aerial transmission of Pneumocystis carinii occurred during cohousing of the rats undergoing gradual ID levels (receivers) with nude rats developing pneumocystosis (seeders). Following contact between receiver and seeder rats, the P. carinii burden of receiver rats was determined by toluidine blue ortho staining and by qPCR targeting the dhfr monocopy gene of this fungus. In this rat model, the level of circulating CD4(+) and CD8(+) T lymphocytes remained significantly stable and different for each dose of dexamethasone tested, thus reaching the goal of a new stable and gradual ID rat model. In addition, an inverse relationship between the P. carinii burden and the level of circulating CD4(+) or CD8(+) T lymphocytes was evidenced. This rat model may be used to study other opportunistic pathogens or even co-infections in a context of gradual ID.

  20. Relationship Between Pneumocystis carinii Burden and the Degree of Host Immunosuppression in an Airborne Transmission Experimental Model.

    PubMed

    Khalife, Sara; Chabé, Magali; Gantois, Nausicaa; Audebert, Christophe; Pottier, Muriel; Hlais, Sani; Pinçon, Claire; Chassat, Thierry; Pierrot, Christine; Khalife, Jamal; Aliouat-Denis, Cécile-Marie; Aliouat, El Moukhtar

    2016-05-01

    To quantitatively assess the risk of contamination by Pneumocystis depending on the degree of immunosuppression (ID) of the exposed rat hosts, we developed an animal model, where rats went through different doses of dexamethasone. Then, natural and aerial transmission of Pneumocystis carinii occurred during cohousing of the rats undergoing gradual ID levels (receivers) with nude rats developing pneumocystosis (seeders). Following contact between receiver and seeder rats, the P. carinii burden of receiver rats was determined by toluidine blue ortho staining and by qPCR targeting the dhfr monocopy gene of this fungus. In this rat model, the level of circulating CD4(+) and CD8(+) T lymphocytes remained significantly stable and different for each dose of dexamethasone tested, thus reaching the goal of a new stable and gradual ID rat model. In addition, an inverse relationship between the P. carinii burden and the level of circulating CD4(+) or CD8(+) T lymphocytes was evidenced. This rat model may be used to study other opportunistic pathogens or even co-infections in a context of gradual ID. PMID:26509699

  1. Little Ice Age versus Present Day: Comparison of Temperature, Precipitation and Seasonality in Speleothem Records from the Han-sur-Lesse Cave, Belgium.

    NASA Astrophysics Data System (ADS)

    Vansteenberge, S.; Van Opdenbosch, J.; Van Rampelbergh, M.; Verheyden, S.; Keppens, E.; Cheng, H.; Edwards, R. L.; Claeys, P. F.

    2015-12-01

    The Proserpine stalagmite is a 2 m large, tabular-shaped speleothem located in the Han-sur-Lesse cave in Belgium. The speleothem formed over the last 1000 years and is still growing. High-accuracy U/Th datings have indicated exceptionally high growth-rates of up to 2 mm per year. This, together with a well expressed annual layering, makes the Proserpine stalagmite an ideal candidate for high-resolution paleoclimate reconstructions of the last millennium. Previous work, including over 10 years of cave monitoring, has already learned us how short-term, i.e. decadal to seasonal, climate variations are incorporated within speleothem calcite from the Han-sur-Lesse cave system. It has been shown that δ18O and δ13C stable isotopes and trace element proxies of recently formed calcite reflect seasonal variations in temperature and precipitation of the near-cave environment (Verheyden et al, 2008; Van Rampelbergh et al., 2014). Now, this knowledge was used to infer local climate parameters further back in time to the period of +/- 1620-1630 CE, corresponding to one of the cold peaks within the Little Ice Age. Speleothem calcite was sampled at sub-annual resolution, with approximately 11 samples per year, for stable isotope analysis. LA-ICP-MS and µXRF analyses resulted in time series of trace elements. Preliminary results indicate a well expressed seasonal signal in δ13C and trace element composition but a multi-annual to decadal trend in δ18O. This combined proxy study eventually enables comparison of the expression of seasonality and longer term climate variations between a Little Ice Age cold peak and Present Day. References: Verheyden, S. et al., 2008, Monitoring climatological, hydrological and geochemical parameters in the Père Noël cave (Belgium): implication for the interpretation of speleothem isotopic and geochemical time-series. International Journal of Speleology, 37(3), 221-234. Van Rampelbergh, M. et al., 2014, Seasonal variations recorded in cave

  2. Grainsize Patterns and Bed Evolution of the Rhone River (France): A Present-day Snapshot Following a Century and a Half of Human Modifications

    NASA Astrophysics Data System (ADS)

    Michal, T.; Parrot, E.; Piegay, H.

    2014-12-01

    Over the past 150 years the Rhône River has been heavily altered by human infrastructures. The first wave (1860 - 1930) of modifications consisted of dikes and groynes designed to narrow the channel and promote incision in order to facilitate navigation. A second period (1948 - 1986) involved the construction of a series of canals and dams for hydroelectricity production. These works bypass multiple reaches of the original channel and drastically reduce the discharge and sediment load reaching them. A comprehensive study underway is aimed at describing the present-day morphology of the Rhone along its 512 km length from its source at Lake Geneva to its sink at the Mediterranean Sea and quantifying the role of management works in the evolution to its current state. Grainsize distributions and armour ratios were determined using a combination of Wolman counts on bars and in shallow channels and dredge samples collected from a boat in navigable reaches. Long profiles were constructed from historical bathymetric maps and bathymetric data collected between 1950 - 2010. Differential long profiles highlighting changes in bed elevation due to sediment storage and erosion were analyzed for three different periods: post-channelization, post-dam construction, and a recent period of major floods. Results show a complex discontinuous pattern in grainsize associated with hydraulic discontinuities imposed by dams. The D50 for bypass reaches is 45 mm compared to a D50 of 34 mm in the non-bypass reaches. The lower D50 as well as a finer tailed distribution in non-bypass reaches reflects fining associated with storage upstream of dams. Armour ratios are on average around 2 but are notably higher for reaches in the middle section of the Rhone. The average incision rate was 1.8 cm/yr for the period of post-channelization and 1.2 cm/yr following dam construction, suggesting the post-dam Rhone was already partially armoured due to incision associated with channelization preceding dam

  3. Present-day stress analysis of the St. Lawrence Lowlands sedimentary basin (Canada) and implications for caprock integrity during CO2 injection operations

    NASA Astrophysics Data System (ADS)

    Konstantinovskaya, E.; Malo, M.; Castillo, D. A.

    2012-01-01

    A geomechanical analysis of the St. Lawrence Lowlands sedimentary basin is important to reliably estimate the maximum sustainable fluid pressures for CO2 injection that will not reactivate pre-existing faults in the caprock thereby inducing a breeched CO2 reservoir. This requires the determination of prevailing stresses (orientations and magnitudes), fault and fractures geometries and rock strengths. The average maximum horizontal stress orientation (SHmax) is estimated N59°E ± 20° in the St. Lawrence Lowlands. The stress orientations were obtained from stress-induced wellbore breakouts inferred from four-arm dipmeter caliper data in 17 wells. These wellbore failure features are confined to Paleozoic lithological units of the St. Lawrence Platform succession and frontal thrusts of the Quebec Appalachians at depths from 250 m to 4 km. Our results are consistent with the regional NE-SW SHmax stress orientation trend that is generally observed in eastern Canada and the U.S. The stresses/pressure gradients estimated for the St. Lawrence Lowlands (depths < 4 km) are: Shmin 20.5 ± 3 kPa/m, Sv 25.6 kPa/m, SHmax 40 ± 7.5 kPa/m, pore pressure Pp 9.8 kPa/m indicating a strike-slip stress regime Shmin < Sv < SHmax. The high-angle NE-SW regional faults and fractures in the Paleozoic sedimentary succession and the Grenvillian basement are oblique to the SHmax stress orientations (10° to 36°) and could be reactivated (slip tendency 0.34 to 0.58) under the present-day stress field if fluid pressures exceeded the critical threshold. Further refinement of regional geomechanical model is required to estimate the maximum sustainable injection pressure necessary for shear reactivation along the regional faults. The regional pore pressure-stress coupling ratio under assumed parameters is about 0.5-0.65 and may contribute to reduce the risk of shear reactivation of faults and fractures. The maximum sustainable Pp that would not cause opening of vertical tensile fractures during

  4. A critical evaluation of present-day and future surface ozone as simulated by global chemistry-climate models in the Atmospheric Chemistry & Climate Model Intercomparison Project (ACCMIP)

    NASA Astrophysics Data System (ADS)

    Schnell, J.; Prather, M. J.

    2014-12-01

    In evaluating a future scenario for air quality, one can identify four major causal factors: (1) global emissions that alter atmospheric composition and thence baseline levels of surface ozone (O3); (2) global changes in climate that also alter these baselines (e.g., temperature, water vapor); (3) climate-driven changes in the meteorological regimes of polluted regions that lead to air quality extreme (AQX) episodes; and (4) changes in the efficacy of local emissions to produce pollution within a governance region. While these factors are all part of a coupled system, a model that combines all would be difficult to verify. Thus an assessment approach would be to evaluate each factor separately using observations and an ensemble of models. In this study, we focus on factor (3), evaluating the ability of the models in the Atmospheric Chemistry & Climate Model Intercomparison Project (ACCMIP) to reproduce the observed present-day climatology (e.g. diurnal/seasonal cycles, AQX episode size) of surface O3 in North America (NA) and Europe (EU). We then characterize future changes within these domains as well as south Asia (SA) for two experiments of RCP8.5 climate, one with O3 precursor emissions representative of the 2100s (RCP8.5) and one representative of the 2000s (Cl2100Em2000). We find that most models simulate the observed climatology well, albeit biased high over the range of each domain's probability distribution (Fig. 1). For RCP8.5, the ensemble mean shows an increase of ~10% in the mean annual maximum daily 8-h average (MDA8) over all domains, with the largest changes in winter months. For Cl2100Em2000, NA shows a small increase (+1%) in annual mean MDA8 while EU and SA show small decreases (-2% and -3%, respectively). Also for RCP8.5, most models show decreases in the mean size (S) and mean duration (D) of AQX episodes in EU (S = -28%, D = -17%) and increases in SA (+54%, +15%). The ensemble mean shows decreases in D (-7%) and increases in S (+21%) in NA

  5. Present-day stress tensors along the southern Caribbean plate boundary zone from inversion of focal mechanism solutions: A successful trial

    NASA Astrophysics Data System (ADS)

    Audemard M., Franck A.; Castilla, Raymi

    2016-11-01

    This paper presents a compilation of 16 present-day stress tensors along the southern Caribbean plate boundary zone (PBZ), and particularly in western and along northern Venezuela. As a trial, these new stress tensors along PBZ have been calculated from inversion of 125 focal mechanism solutions (FMS) by applying the Angelier & Mechler's dihedral method, which were originally gathered by the first author and published in 2005. These new tensors are compared to those 59 tensors inverted from fault-slip data measured only in Plio-Quaternary sedimentary rocks, compiled in Audemard et al. (2005), which were originally calculated by several researchers through the inversion methods developed by Angelier and Mechler or Etchecopar et al. The two sets of stress tensors, one derived from geological data and the other one from seismological data, compare very well throughout the PBZ in terms of both stress orientation and shape of the stress tensor. This region is characterized by a compressive strike-slip (transpressional senso lato), occasionally compressional, regime from the southern Mérida Andes on the southwest to the gulf of Paria in the east. Significant changes in direction of the maximum horizontal stress (σH = σ1) can be established along it though. The σ1 direction varies progressively from nearly east-west in the southern Andes (SW Venezuela) to between NW-SE and NNW-SSE in northwestern Venezuela; this direction remaining constant across northern Venezuela, from Colombia to Trinidad. In addition, the σV defined by inversion of focal mechanisms or by the shape of the stress ellipsoid derived from the Etchecopar et al.'s method better characterize whether the stress regime is transpressional or compressional, or even very rarely trantensional at local scale. The orientation and space variation of this regional stress field in western Venezuela results from the addition of the two major neighbouring interplate maximum horizontal stress orientations (

  6. Evaluation and intercomparison of downscaled daily precipitation indices over Japan in present-day climate: Strengths and weaknesses of dynamical and bias correction-type statistical downscaling methods

    NASA Astrophysics Data System (ADS)

    Iizumi, Toshichika; Nishimori, Motoki; Dairaku, Koji; Adachi, Sachiho A.; Yokozawa, Masayuki

    2011-01-01

    In this study, we evaluate the accuracy of four regional climate models (NHRCM, NRAMS, TRAMS, and TWRF) and one bias correction-type statistical model (CDFDM) for daily precipitation indices under the present-day climate (1985-2004) over Japan on a 20 km grid interval. The evaluated indices are (1) mean precipitation, (2) number of days with precipitation ≥1 mm/d (corresponds to number of wet days), (3) mean amount per wet day, (4) 90th percentile of daily precipitation, and (5) number of days with precipitation ≥90th percentile of daily precipitation. The boundary conditions of the dynamical models and the predictors of the statistical model are given from the single reanalysis data, i.e., JRA25. Both types of models successfully improved the accuracy of the indices relative to the reanalysis data in terms of bias, seasonal cycle, geographical pattern, cumulative distribution function of wet-day amount, and interannual variation pattern. In most aspects, NHRCM is the best model of all indices. Through the intercomparison between the dynamical and statistical models, respective strengths and weaknesses emerged. Briefly, (1) many dynamical models simulate too many wet days with a small amount of precipitation in humid climate zones, such as summer in Japan, relative to the statistical model, unless the cumulus convection scheme improved for such a condition is incorporated; (2) a few dynamical models can derive a better high-order percentile of daily precipitation (e.g., 90th percentile) than the statistical model; (3) both the dynamical and statistical models are still insufficient in the representation of the interannual variation pattern of the number of days with precipitation ≥90th percentile of daily precipitation; (4) the statistical model is comparable to the dynamical models in the long-term mean geographical pattern of the indices even on a 20 km grid interval if a dense observation network is applicable; (5) the statistical model is less accurate

  7. Aerosol Direct, Indirect, Semidirect, and Surface Albedo Effects from Sector Contributions Based on the IPCC AR5 Emissions for Preindustrial and Present-day Conditions

    NASA Technical Reports Server (NTRS)

    Bauer, Susanne E.; Menon, Surabi

    2012-01-01

    The anthropogenic increase in aerosol concentrations since preindustrial times and its net cooling effect on the atmosphere is thought to mask some of the greenhouse gas-induced warming. Although the overall effect of aerosols on solar radiation and clouds is most certainly negative, some individual forcing agents and feedbacks have positive forcing effects. Recent studies have tried to identify some of those positive forcing agents and their individual emission sectors, with the hope that mitigation policies could be developed to target those emitters. Understanding the net effect of multisource emitting sectors and the involved cloud feedbacks is very challenging, and this paper will clarify forcing and feedback effects by separating direct, indirect, semidirect and surface albedo effects due to aerosols. To this end, we apply the Goddard Institute for Space Studies climate model including detailed aerosol microphysics to examine aerosol impacts on climate by isolating single emission sector contributions as given by the Coupled Model Intercomparison Project Phase 5 (CMIP5) emission data sets developed for Intergovernmental Panel on Climate Change (IPCC) AR5. For the modeled past 150 years, using the climate model and emissions from preindustrial times to present-day, the total global annual mean aerosol radiative forcing is -0.6 W/m(exp 2), with the largest contribution from the direct effect (-0.5 W/m(exp 2)). Aerosol-induced changes on cloud cover often depends on cloud type and geographical region. The indirect (includes only the cloud albedo effect with -0.17 W/m(exp 2)) and semidirect effects (-0.10 W/m(exp 2)) can be isolated on a regional scale, and they often have opposing forcing effects, leading to overall small forcing effects on a global scale. Although the surface albedo effects from aerosols are small (0.016 W/m(exp 2)), triggered feedbacks on top of the atmosphere (TOA) radiative forcing can be 10 times larger. Our results point out that each

  8. Evaluation of preindustrial to present-day black carbon and its albedo forcing from Atmospheric Chemistry and Climate Model Intercomparison Project (ACCMIP)

    NASA Technical Reports Server (NTRS)

    Lee, Y. H.; Lamarque, J.-F.; Flanner, M. G.; Jiao, C.; Shindell, D. T.; Bernsten, T.; Bisiaux, M. M.; Cao, J.; Collins, W. J.; Curran, M.; Edwards, R.; Faluvegi, G.; Ghan, S.; Horowitz, L. W.; McConnell, J. R.; Ming, J.; Myhre, G.; Nagashima, T.; Naik, V.; Rumbold, S. T.; Skeie, R. B.; Sudo, K.; Takemura, T.; Thevenon, F.; Xu, B.; Yoon, J.-H.

    2013-01-01

    As part of the Atmospheric Chemistry and Climate Model Intercomparison Project (ACCMIP), we evaluate the historical black carbon (BC) aerosols simulated by 8 ACCMIP models against observations including 12 ice core records, long-term surface mass concentrations, and recent Arctic BC snowpack measurements. We also estimate BC albedo forcing by performing additional simulations using offline models with prescribed meteorology from 1996-2000. We evaluate the vertical profile of BC snow concentrations from these offline simulations using the recent BC snowpack measurements. Despite using the same BC emissions, the global BC burden differs by approximately a factor of 3 among models due to differences in aerosol removal parameterizations and simulated meteorology: 34 Gg to 103 Gg in 1850 and 82 Gg to 315 Gg in 2000. However, the global BC burden from preindustrial to present-day increases by 2.5-3 times with little variation among models, roughly matching the 2.5-fold increase in total BC emissions during the same period.We find a large divergence among models at both Northern Hemisphere (NH) and Southern Hemisphere (SH) high latitude regions for BC burden and at SH high latitude regions for deposition fluxes. The ACCMIP simulations match the observed BC surface mass concentrations well in Europe and North America except at Ispra. However, the models fail to predict the Arctic BC seasonality due to severe underestimations during winter and spring. The simulated vertically resolved BC snow concentrations are, on average, within a factor of 2-3 of the BC snowpack measurements except for Greenland and the Arctic Ocean. For the ice core evaluation, models tend to adequately capture both the observed temporal trends and the magnitudes at Greenland sites. However, models fail to predict the decreasing trend of BC depositions/ice core concentrations from the 1950s to the 1970s in most Tibetan Plateau ice cores. The distinct temporal trend at the Tibetan Plateau ice cores

  9. Lyα-emitting Galaxies at z = 2.1 in ECDF-S: Building Blocks of Typical Present-day Galaxies?

    NASA Astrophysics Data System (ADS)

    Guaita, Lucia; Gawiser, Eric; Padilla, Nelson; Francke, Harold; Bond, Nicholas A.; Gronwall, Caryl; Ciardullo, Robin; Feldmeier, John J.; Sinawa, Shawn; Blanc, Guillermo A.; Virani, Shanil

    2010-05-01

    ) = 11.5+0.4 -0.5, which are among the lowest mass halos yet probed at this redshift. We used the Sheth and Tormen conditional mass function to study the descendants of these LAEs and found that their typical present-day descendants are local galaxies with L* properties, like the Milky Way. Based on observations obtained at Cerro Tololo Inter-American Observatory, a division of the National Optical Astronomy Observatory, which is operated by the Association of Universities for Research in Astronomy, Inc., under cooperative agreement with the National Science Foundation.

  10. Evaluation of preindustrial to present-day black carbon and its albedo forcing from Atmospheric Chemistry and Climate Model Intercomparison Project (ACCMIP)

    NASA Astrophysics Data System (ADS)

    Lee, Y. H.; Lamarque, J.-F.; Flanner, M. G.; Jiao, C.; Shindell, D. T.; Berntsen, T.; Bisiaux, M. M.; Cao, J.; Collins, W. J.; Curran, M.; Edwards, R.; Faluvegi, G.; Ghan, S.; Horowitz, L. W.; McConnell, J. R.; Ming, J.; Myhre, G.; Nagashima, T.; Naik, V.; Rumbold, S. T.; Skeie, R. B.; Sudo, K.; Takemura, T.; Thevenon, F.; Xu, B.; Yoon, J.-H.

    2013-03-01

    As part of the Atmospheric Chemistry and Climate Model Intercomparison Project (ACCMIP), we evaluate the historical black carbon (BC) aerosols simulated by 8 ACCMIP models against observations including 12 ice core records, long-term surface mass concentrations, and recent Arctic BC snowpack measurements. We also estimate BC albedo forcing by performing additional simulations using offline models with prescribed meteorology from 1996-2000. We evaluate the vertical profile of BC snow concentrations from these offline simulations using the recent BC snowpack measurements. Despite using the same BC emissions, the global BC burden differs by approximately a factor of 3 among models due to differences in aerosol removal parameterizations and simulated meteorology: 34 Gg to 103 Gg in 1850 and 82 Gg to 315 Gg in 2000. However, the global BC burden from preindustrial to present-day increases by 2.5-3 times with little variation among models, roughly matching the 2.5-fold increase in total BC emissions during the same period. We find a large divergence among models at both Northern Hemisphere (NH) and Southern Hemisphere (SH) high latitude regions for BC burden and at SH high latitude regions for deposition fluxes. The ACCMIP simulations match the observed BC surface mass concentrations well in Europe and North America except at Ispra. However, the models fail to predict the Arctic BC seasonality due to severe underestimations during winter and spring. The simulated vertically resolved BC snow concentrations are, on average, within a factor of 2-3 of the BC snowpack measurements except for Greenland and the Arctic Ocean. For the ice core evaluation, models tend to adequately capture both the observed temporal trends and the magnitudes at Greenland sites. However, models fail to predict the decreasing trend of BC depositions/ice core concentrations from the 1950s to the 1970s in most Tibetan Plateau ice cores. The distinct temporal trend at the Tibetan Plateau ice cores

  11. Late Miocene to present-day exhumation and uplift of the Internal Zone of the Rif chain: Insights from low temperature thermochronometry and basin analysis

    NASA Astrophysics Data System (ADS)

    Romagny, A.; Ph. Münch; Cornée, J.-J.; Corsini, M.; Azdimousa, A.; Melinte-Dobrinescu, M. C.; Drinia, H.; Bonno, M.; Arnaud, N.; Monié, P.; Quillévéré, F.; Ben Moussa, A.

    2014-07-01

    Located on the margin of the west Alboran basin, the Gibraltar Arc (Betic-Rif mountain belt) displays post-Pliocene vertical movements evidenced by uplifted marine sedimentary basins and marine terraces. Quantification of vertical movements is an important clue to understand the origin of present-day relief generation in the Betic-Rif mountain chain together with the causes of the Messinian Salinity Crisis. In this paper, we present the results of a pluridisciplinary study combining an analysis of low temperature thermochronology and Pliocene basins evolution to constrain the exhumation history and surface uplift of internals units of the Rif belt (Northern Morocco). The mean (U-Th)/He apatite ages obtained from 11 samples are comprised between 14.1 and 17.8 Ma and display a wide dispersion, which could be explained by a great variability of apatite chemistries in the analyzed samples. No correlations between altitude and age have been found along altitudinal profile suggesting a rapid exhumation during this period. Thermal modeling using our (U-Th)/He apatite ages and geochronological data previously obtained in the same area (40Ar/39Ar and K/Ar data on biotite, zircon and apatite fission track) allow us to propose a cooling history. The rocks suffered a rapid cooling at 60-100 °C/Ma between 22.5 and 19 Ma, then cooled to temperatures around 40 °C between 19 and 18 Ma. They were re-heated at around 110 °C between 18 and 15 Ma then rapidly cooled and exhumed to reach the surface temperature at around 13 Ma. The re-heating could be related to a renewal in thrusting and burying of the inner zones. Between 15 and 13 Ma the cooling resumed at a rate of 50 °C/Ma indicating an exhumation rate of 0.8 mm/y considering an average 40 °C/km geothermal gradient. This exhumation may be linked to the extension in the Alboran Sea. Otherwise biostratigraphic and sedimentological analysis of Pliocene basins of the internal Rif provided informations on the more recent events

  12. Hepatitis E genotype 4 virus from feces of monkeys infected experimentally can be cultured in PLC/PRF/5 cells and upregulate host interferon-inducible genes.

    PubMed

    Zhang, Feng; Qi, Ying; Harrison, Tim J; Luo, Baobin; Zhou, Yan; Li, Xiuhua; Song, Aijing; Huang, Weijin; Wang, Youchun

    2014-10-01

    The understanding of the interaction between hepatitis E virus (HEV) and its host cells has been impeded greatly by the absence of a cell culture system. In this study, an efficient cultivation method was developed in PLC/PRF/5 cells for HEV genotype 4 from the feces of monkeys infected experimentally. Compared to minimal essential medium (MEM), mixed Dulbecco's Modified Eagle's Medium (DMEM)/M199 improved the infection efficiency of HEV in PLC/PRF/5 cells. The incubation time and temperature were set at 6 hr and 40°C, respectively. Compared to a 100% ELISA positive ratio (EPR) of 1 × 10(6)  copies/ml HEV inoculated flasks, the ELISA positive ratio was 100%, 75%, 37.5%, and 100% for flasks inoculated with HEV incubated for 30 min under the conditions of pH 3.0, pH 11.0, 56°C and delipidation treatment, respectively. Gene expression profiles of HEV inoculated and control PLC/PRF/5 cells were assayed using a microarray. Four interferon-inducible genes, IFI27, IFI6, Mx1, and CMPK2, were up-regulated during HEV-infection. Furthermore, the replication of HEV was inhibited at 3-14 days after treatment with 500 IU/ml IFN-α2b.

  13. Increased expression of host iron-binding proteins precedes iron accumulation and calcification of primary lung lesions in experimental tuberculosis in the guinea pig.

    PubMed

    Basaraba, Randall J; Bielefeldt-Ohmann, Helle; Eschelbach, Ellie K; Reisenhauer, Claire; Tolnay, Airn E; Taraba, Lauren C; Shanley, Crystal A; Smith, Erin A; Bedwell, Cathy L; Chlipala, Elizabeth A; Orme, Ian M

    2008-01-01

    The growth and virulence of Mycobacterium tuberculosis depends on its ability to scavenge host iron, an essential and limited micronutrient in vivo. In this study, we show that ferric iron accumulates both intra- and extra-cellularly in the primary lung lesions of guinea pigs aerosol-infected with the H37Rv strain of M. tuberculosis. Iron accumulated within macrophages at the periphery of the primary granulomatous lesions while extra-cellular ferric iron was concentrated in areas of lesion necrosis. Accumulation of iron within primary lesions was preceded by an increase in expression of heavy chain (H) ferritin, lactoferrin and receptors for transferrin, primarily by macrophages and granulocytes. The increased expression of intra-cellular H ferritin and extra-cellular lactoferrin, more so than transferrin receptor, paralleled the development of necrosis within primary lesions. The deposition of extra-cellular ferric iron within necrotic foci coincided with the accumulation of calcium and phosphorus and other cations in the form of dystrophic calcification. Primary lung lesions from guinea pigs vaccinated with Mycobactrium bovis BCG prior to experimental infection, had reduced iron accumulation as well as H ferritin, lactoferrin and transferrin receptor expression. The amelioration of primary lesion necrosis and dystrophic calcification by BCG vaccination was coincident with the lack of extra-cellular ferric iron and lactoferrin accumulation. These data demonstrate that BCG vaccination ameliorates primary lesion necrosis, dystrophic mineralization and iron accumulation, in part by down-regulating the expression of macrophage H ferritin, lactoferrin and transferrin receptors, in vivo.

  14. Geochemical evidence of present-day serpentinization

    USGS Publications Warehouse

    Barnes, I.; LaMarche, Valmore C.; Himmelberg, G.

    1967-01-01

    Ultrabasic (pH > 11) water issues from some fresh ultramafic bodies. The properties of the ultrabasic solutions are believed to be due to current reactions yielding serpentine from primary olivines and pyroxenes. The low concentrations of divalent iron, divalent magnesium, and dissolved silica from the serpentinization require an increase in rock volume.

  15. Cosmology: from Pomeranchuk to the Present Day

    NASA Astrophysics Data System (ADS)

    Dolgov, A. D.

    A short and, due to lack of time (half a century of the science progress in half an hour), rather superficial review on the development of cosmology for scientists working on particle and nuclear physics is presented. The introductory historical part is mostly dedicated to the fundamental works done in Russia (USSR), but not always well known outside the country. Next, the key papers on theory and on astronomical observations, which determined the progress in cosmology during the last half-century or posed crucial problem, are discussed. Among them there are inflation, baryogenesis, dark matter, dark energy, the vacuum energy problem, modification of gravity at large scales, and angular fluctuations of the cosmic microwave background radiation. The presentation is probably biased towards cosmology as it is seen from Russia (or from ITEP) and reflects personal prejudice of the author.

  16. [Present day Marxist sociology and psychoanalysis].

    PubMed

    Biran, S

    1979-01-01

    The main common theme in psychoanalysis and Marxist sociology is the understanding that it is not consciousness that determines being, but being (spiritual, social) that determines consciousness. The different variations of Marxist movements today are in fact distant from Marx's theory of sociology. They have become representatives of utopian socialism, using anarchistic methods to achieve that aim. This development can only be understood as a social neurosis, with the narcistic frustation of the intellectual class as its cause, and grandiose claims, intolerance, dogmatic thinking and destructive behaviour as its symptoms. The only justified criticism of psychoanalysis from the pseudo-Marxist side is based on the imperfection and error in the analytical doctrine of superego. This should be replaced by the idea of conscious, subjective, emotional morality which clearly explains the aggression contained in social structures. PMID:498762

  17. Global three-dimensional model calculations of the budgets and present-day atmospheric lifetimes of CF2ClCFCl2 (CFC-113) and CHClF2 (CFC-22)

    NASA Technical Reports Server (NTRS)

    Golombek, Amram; Prinn, Ronald G.

    1989-01-01

    The annual percentage increases in concentrations of the chlorofluorocarbons CFC-113 (an industrial solvent) and CFC-22 (a refrigerant) are the highest among major chlorofluorocarbons in the atmosphere today. The present-day atmospheric lifetimes for these species are computed using a global three-dimensional dynamical-chemical model. The present-day lifetimes of both are long (15.5 years for CFC-22 and 136 or 195 years for CFC-113, depending on assumed O2 absorption cross sections), underscoring the need to decrease their emissions in order to minimize their future role in ozone destruction and greenhouse warming.

  18. Vapor-Liquid Partitioning of Iron and Manganese in Hydrothermal Fluids: An Experimental Investigation with Application to the Integrated Study of Basalt-hosted Hydrothermal Systems

    NASA Astrophysics Data System (ADS)

    Pester, N. J.; Seyfried, W. E.

    2010-12-01

    The chemistry of deep-sea hydrothermal vent fluids, expressed at the seafloor, reflects a complex history of physicochemical reactions. After three decades of field and experimental investigations, the processes of fluid-mineral equilibria that transform seawater into that of a typical “black smoker” are generally well described in the literature. Deep crustal fluids, when encountering a given heat source that ultimately drives hydrothermal circulation, routinely intersect the two-phase boundary. This process results in the nearly ubiquitous observations of variable salinity in vent fluids and is often a secondary driver of circulation via the evolution of a more buoyant (i.e. less saline) phase. Phase separation in chemically complex fluids results in the partitioning of dissolved species between the two evolved phases that deviates from simple charge balance calculations and these effects become more prominent with increasing temperature and/or decreasing pressure along the two-phase envelope. This process of partitioning has not been extensively studied and the interplay between the effects of phase separation and fluid-mineral equilibrium are not well understood. Most basalt-hosted hydrothermal systems appear to enter a steady state mode wherein fluids approach the heat source at depth and rise immediately once the two-phase boundary is met. Thus, venting fluids exhibit only modest deviations from seawater bulk salinity and the effects of partitioning are likely minor for all but the most volatile elements. Time series observations at integrated study sites, however, demonstrate dynamic changes in fluid chemistry following eruptions/magmatic events, including order of magnitude increases in gas concentrations and unexpectedly high Fe/Cl ratios. In this case, the time dependence of vapor-liquid partitioning relative to fluid-mineral equilibrium must be considered when attempting to interpret changes in subsurface reaction conditions. The two-phase region of

  19. Effect of hosts on competition among clones and evidence of differential selection between pathogenic and saprophytic phases in experimental populations of the wheat pathogen Phaeosphaeria nodorum

    PubMed Central

    2011-01-01

    Background Monoculture, multi-cropping and wider use of highly resistant cultivars have been proposed as mechanisms to explain the elevated rate of evolution of plant pathogens in agricultural ecosystems. We used a mark-release-recapture experiment with the wheat pathogen Phaeosphaeria nodorum to evaluate the impact of two of these mechanisms on the evolution of a pathogen population. Nine P. nodorum isolates marked with ten microsatellite markers and one minisatellite were released onto five replicated host populations to initiate epidemics of Stagonospora nodorum leaf blotch. The experiment was carried out over two consecutive host growing seasons and two pathogen collections were made during each season. Results A total of 637 pathogen isolates matching the marked inoculants were recovered from inoculated plots over two years. Genetic diversity in the host populations affected the evolution of the corresponding P. nodorum populations. In the cultivar mixture the relative frequencies of inoculants did not change over the course of the experiment and the pathogen exhibited a low variation in selection coefficients. Conclusions Our results support the hypothesis that increasing genetic heterogeneity in host populations may retard the rate of evolution in associated pathogen populations. Our experiment also provides indirect evidence of fitness costs associated with host specialization in P. nodorum as indicated by differential selection during the pathogenic and saprophytic phases. PMID:21718545

  20. Pretreatment with antilymphocyte globulin and donor cells on graft prolongation in an experimental model and some observations on graft-versus-host reaction.

    PubMed

    Pegrum, G D; Williams, G; Markwick, J; Barnes, R M

    1976-01-01

    Survival of Lewis X Brown Norway F1 renal allografts was prolonged in Lewis recipients by pretreatment with small numbers of donor marrow cells and low dose antilymphocyte globulin(ALG)in combination. Either marrow cells or ALG alone were ineffective at these doses. It was also shown that pretreatment with marrow cells and antilymphocyte serum (ALS) considerably suppressed the local graft-versus-host reaction.

  1. Experimental infections with pathogenic free-living amebae in laboratory primate hosts: I (A) A study on susceptibility to Naegleria fowleri.

    PubMed

    Wong, M M; Karr, S L; Balamuth, W B

    1975-04-01

    Studies were conducted on 27 Old World monkeys to determine their susceptibility to pathogenic strains (HB-1 and C-66) of Naegleria fowleri by intranasal, intravenous, or intrathecal inoculation of trophozoites. No clinically detectable disease resulted from either intranasal or intravenous inoculation, but 11 of 18 monkeys inoculated intrathecally succumed to acutely fatal meningoencephalitis, while the other 7 survived with no obvious permanent brain damage. Pathogenicity of N. fowleri appeared to be influenced by the strain virulence, growth phase, and cultural condition of the amebae, as well as age, immune competence, and other as yet unknown host factors. PMID:805226

  2. Experimental infections with pathogenic free-living amebae in laboratory primate hosts: I (A) A study on susceptibility to Naegleria fowleri.

    PubMed

    Wong, M M; Karr, S L; Balamuth, W B

    1975-04-01

    Studies were conducted on 27 Old World monkeys to determine their susceptibility to pathogenic strains (HB-1 and C-66) of Naegleria fowleri by intranasal, intravenous, or intrathecal inoculation of trophozoites. No clinically detectable disease resulted from either intranasal or intravenous inoculation, but 11 of 18 monkeys inoculated intrathecally succumed to acutely fatal meningoencephalitis, while the other 7 survived with no obvious permanent brain damage. Pathogenicity of N. fowleri appeared to be influenced by the strain virulence, growth phase, and cultural condition of the amebae, as well as age, immune competence, and other as yet unknown host factors.

  3. Two nucleotide positions in the Citrus exocortis viroid RNA associated with symptom expression in Etrog citron but not in experimental herbaceous hosts.

    PubMed

    Murcia, Nubia; Bernad, Lucía; Duran-Vila, Núria; Serra, Pedro

    2011-02-01

    Citrus exocortis viroid (CEVd) is the causal agent of exocortis disease of citrus. CEVd has a wide host range that includes woody and herbaceous species. A new CEVd strain (CEVd(COL)), phylogenetically clustering with CEVd variants of Class A inducing severe symptoms in tomato, was identified in Colombia and shown to induce only extremely mild symptoms in Etrog citron indicator plants. Using site-directed mutagenesis, two nucleotide substitutions (314A → G and 315U → A) in the lower strand of the P domain of the predicted CEVd(COL) secondary structure resulted in a severe artificial CEVd(MCOL) variant. Conversely, two nucleotide exchanges (314G → A and 315A → U) in the same region of the severe variant CEVd(E-117) resulted in a symptomless artificial CEVd(ME-117) variant. Infectivity assays conducted with the natural and mutated variants showed that all induced severe symptoms in Gynura aurantiaca, tomato and chrysanthemum. This is the first report of the identification of pathogenic determinants of CEVd in citrus, and shows that these pathogenicity determinants are host dependent.

  4. Using present-day patterns of interseismic coupling to model the C.E. 1707 Hōei earthquake and simulate tsunami inundation of Lake Ryuuoo in the Bungo Channel, southwestern Japan

    NASA Astrophysics Data System (ADS)

    Baranes, H. E.; Woodruff, J. D.; Loveless, J. P.; Cheng, W.; Weiss, R.; Kanamaru, K.

    2015-12-01

    The C.E. 1707 Hōei event is often considered the worst-case scenario for a Nankai Trough earthquake and tsunami impacting southwestern Japan, and recent estimates of the earthquake's magnitude have exceeded MW 9. However, when paired with tsunami simulations, previously published earthquake models for the event fail to match sedimentological and historical records of 1707 tsunami height in Shikoku and Kyushu. Specifically, models do not produce a sufficiently large tsunami in the northern Bungo Channel and Seto Inland Sea without also over-predicting tsunami heights along the open Pacific coastlines of Shikoku and Kyushu. Here, we apply a newly developed rupture model that uses present-day patterns of geodetically imaged interseismic coupling to inform patterns in coseismic slip. Along the southwestern extent of the plate interface (the Hyuga-nada area), there is a region of weak coupling up-dip along the trench axis and a region of strong coupling down-dip beneath Shikoku and Kyushu. Following this pattern, the new earthquake model produces less coseismic uplift offshore and greater subsidence in an inland region that includes the Bungo Channel. This combination of regional subsidence and a tsunami wave more focused to the Bungo Channel results in inundation patterns more consistent with historical and sedimentological observations in the Hyuga-nada area. We also run the tsunami simulation on a high-resolution grid around Lake Ryuuoo, a back-barrier lake in the northern Bungo Channel that contains a marine overwash deposit from the 1707 tsunami. We apply a simple sediment transport model to demonstrate that the coupling-based rupture scenario produces flow over Lake Ryuuoo's barrier capable of transporting the maximum grain size observed in the lake's 1707 deposit. These findings suggest that spatial trends in our present-day coupling model are more consistent with inundation patterns observed for large tsunamis generated by coseismic rupture along the Nankai

  5. Heterogeneous pathological outcomes after experimental pH1N1 influenza infection in ferrets correlate with viral replication and host immune responses in the lung.

    PubMed

    Vidaña, Beatriz; Martínez, Jorge; Martínez-Orellana, Pamela; García Migura, Lourdes; Montoya, María; Martorell, Jaime; Majó, Natàlia

    2014-01-01

    The swine-origin pandemic (p) H1N1 influenza A virus causes mild upper-respiratory tract disease in most human patients. However, some patients developed severe lower-respiratory tract infections with fatal consequences, and the cause of these infections remain unknown. Recently, it has been suggested that different populations have different degrees of susceptibility to pH1N1 strains due to host genetic variations that are associated with inappropriate immune responses against viral genetic characteristics. Here, we tested whether the pathologic patterns of influenza strains that produce different disease outcomes in humans could be reproduced in a ferret model. Our results revealed that the severities of infection did not correspond to particular viral isolate and were not associated with the clinical phenotypes of the corresponding patients. Severe pathological outcomes were associated with higher viral replication, especially in alveolar areas, and with an exacerbated innate cellular immune response that was characterised by substantial phagocytic and cytotoxic cell migration into the lungs. Moreover, detrimental innate cellular responses were linked to the up-regulation of several proinflammatory cytokines and chemokines and the down-regulation of IFNα in the lungs. Additionally, severe lung lesions were associated with greater up-regulations of pro-apoptotic markers and higher levels of apoptotic neutrophils and macrophages. In conclusion, this study confirmed that the clinicopathological outcomes of pH1N1 infection in ferrets were not only due to viral replication abilities but also depended on the hosts' capacities to mount efficient immune responses to control viral infection of the lung.

  6. Seafloor morphology of the Eurasia-Nubia (Africa) plate boundary between the Tore-Madeira Rise and the Straits of Gibraltar: a case of coexistent Mesozoic through Present day features of tectonic, oceanographic and sedimentary origin

    NASA Astrophysics Data System (ADS)

    Terrinha, Pedro; Duarte, João.; Valadares, Vasco; Batista, Luis; Zitellini, Nevio; Grácia, Eulalia; Lourenço, Nuno; Rosas, Filipe; Roque, Cristina

    2010-05-01

    The joint use of more than 10.000 km multichannel seismic reflection profiles and 180.000km2 of multibeam swath bathymetry and backscatter allowed for a new vision of the seafloor tectonic and geomorphic processes of the area that encompasses the present day plate boundary between Africa and Eurasia, between the Gibraltar Straits and the Tore-Madeira Rise, in the southern sector of the North Atlantic Ocean. The interpretation of this data allowed for the detailed description of the seafloor morphology (i.e. a morphologic map) and the classification of the morphologic features in what respects the genetic process and age. It can be seen that in the same region coexist morphologic features that result from tectonic processes associated with the Triassic-Cretaceous break-up of Pangea, the Paleogene-Miocene compressive phase, the Miocene through Present subduction under the Gibraltar Arc (Gutscher et al., 2002), the Pliocene-Quaternary wrench tectonics and possible coeval plate boundary (Zitellini et al., 2009), the Present day mud volcanism and propagation of the compressive deformation along the West Continental Margin of Portugal (Terrinha et al., 2009). Interpretation of the seismic profiles together with the bathymetry allows the understanding of endogenous and exogenous processes that creates reliefs associated with active structures (related to the Miocene through Present compressive stress field). Other reliefs generated in Mesozoic times by analogous processes can be as well preserved as these active ones. In what concerns exogenous processes, the analysis of the two datasets (reflection seismics and bathymetry) allowed for the description of morphologic features associated with oceanic currents that interact with the seafloor forming these important features. As is the case of the well known active contourites but also less known features, like giant scours at 4 km water depth that have recently been described, suggesting the interaction of deep currents and

  7. Enhancement of Experimental Cutaneous Leishmaniasis by Leishmania Molecules Is Dependent on Interleukin-4, Serine Protease/Esterase Activity, and Parasite and Host Genetic Backgrounds ▿

    PubMed Central

    Silva, Virgínia M. G.; Larangeira, Daniela F.; Oliveira, Pablo R. S.; Sampaio, Romina B.; Suzart, Paula; Nihei, Jorge S.; Teixeira, Márcia C. A.; Mengel, José O.; dos-Santos, Washington L. C.; Pontes-de-Carvalho, Lain

    2011-01-01

    Most inbred strains of mice, like the BALB/c strain, are susceptible to Leishmania amazonensis infections and resistant to Leishmania braziliensis infections. This parasite-related difference could result from the activity of an L. amazonensis-specific virulence factor. In agreement with this hypothesis, it is shown here that the intravenous injection of BALB/c mice with L. amazonensis amastigote extract (LaE) but not the L. braziliensis extract confers susceptibility to L. braziliensis infection. This effect was associated with high circulating levels of IgG1 anti-L. amazonensis antibodies and with an increase in interleukin-4 (IL-4) production and a decrease in gamma interferon production by draining lymph node cells. Moreover, the effect was absent in IL-4-knockout mice. The biological activity in the LaE was not mediated by amphiphilic molecules and was inhibited by pretreatment of the extract with irreversible serine protease inhibitors. These findings indicate that the LaE contains a virulence-related factor that (i) enhances the Leishmania infection by promoting Th2-type immune responses, (ii) is not one of the immunomodulatory Leishmania molecules described so far, and (iii) is either a serine protease or has an effect that depends on that protease activity. In addition to being Leishmania species specific, the infection-enhancing activity was also shown to depend on the host genetic makeup, as LaE injections did not affect the susceptibility of C57BL/6 mice to L. braziliensis infection. The identification of Leishmania molecules with infection-enhancing activity could be important for the development of a vaccine, since the up- or downmodulation of the immune response against a virulence factor could well contribute to controlling the infection. PMID:21173308

  8. Effects of single or trickle Haemonchus contortus experimental infection on digestibility and host responses of naïve Creole kids reared indoor.

    PubMed

    Bambou, J C; Cei, W; Camous, S; Archimède, H; Decherf, A; Philibert, L; Barbier, C; Mandonnet, N; González-García, E

    2013-01-31

    The objective of this study was to compare the effects of the type of Haemonchus contortus experimental infection (trickle infection, TI versus single infection, SI) on feed intake, nutrients digestibility, parasitological and haematological measures, and plasma leptin in Creole kids. The animals were infected over 2 periods (challenge 1 and challenge 2) of 6 weeks each, corresponding respectively to the primary and the secondary infection. Periods prior infection (1 week each) were considered as controls. The primary infection was realized with 35 Creole kids (18.40±3.76 kg BW) housed in individual boxes and fed a hay-based diet. The secondary infection continued with 29 kids (21.90±3.40 kg BW) from the initial 35. A total of 6 kids and 8 kids were slaughtered for measuring nematode burden at the end of the primary and the secondary infection, respectively. Measurements of nutrients digestibility were made at 0, 3 and 5 weeks post-infection for both challenges. Faecal egg count (FEC), blood eosinophilia and packed cell volume (PCV) were monitored weekly. Feed intake (dry matter intake, DMI) and nutrients digestibility were negatively affected by H. contortus infection only during the primary infection. Plasma leptin changed significantly over time (P=0.0002) but was not affected by the infection type. Effect of infection type was observed only on crude protein digestibility during the primary infection, which was lower in the TI group (P<0.01). The overall level of blood eosinophilia was significantly higher in the TI group (P<0.0001) during both challenges. The overall FEC mean was significantly higher in the SI compared with the TI groups, during both challenges (P<0.02). These results were related to the mean female length significantly higher in the SI group compared with the TI group during challenge 1 (P=0.004), and the number of adult nematode significantly lower in the TI group compared with the SI group during the challenge 2 (P=0.05). The results

  9. Differential Effect of MyD88 Signal in Donor T Cells on Graft-versus-Leukemia Effect and Graft-versus-Host Disease after Experimental Allogeneic Stem Cell Transplantation.

    PubMed

    Lim, Ji-Young; Ryu, Da-Bin; Lee, Sung-Eun; Park, Gyeongsin; Choi, Eun Young; Min, Chang-Ki

    2015-11-01

    Despite the presence of toll like receptor (TLR) expression in conventional TCRαβ T cells, the direct role of TLR signaling via myeloid differentiation factor 88 (MyD88) within T lymphocytes on graft-versus-host disease (GVHD) and graft-versus-leukemia (GVL) effect after allogeneic stem cell transplantation (allo-SCT) remains unknown. In the allo-SCT model of C57BL/6 (H-2(b)) → B6D2F1 (H-2(b/d)), recipients received transplants of wild type (WT) T-cell-depleted (TCD) bone marrow (BM) and splenic T cells from either WT or MyD88 deficient (MyD88KO) donors. Host-type (H-2(d)) P815 mastocytoma or L1210 leukemia cells were injected either subcutaneously or intravenously to generate a GVHD/GVL model. Allogeneic recipients of MyD88KO T cells demonstrated a greater tumor growth without attenuation of GVHD severity. Moreover, GVHD-induced GVL effect, caused by increasing the conditioning intensity was also not observed in the recipients of MyD88KO T cells. In vitro, the absence of MyD88 in T cells resulted in defective cytolytic activity to tumor targets with reduced ability to produce IFN-γ or granzyme B, which are known to critical for the GVL effect. However, donor T cell expansion with effector and memory T-cell differentiation were more enhanced in GVHD hosts of MyD88KO T cells. Recipients of MyD88KO T cells experienced greater expansion of Foxp3- and IL4-expressing T cells with reduced INF-γ producing T cells in the spleen and tumor-draining lymph nodes early after transplantation. Taken together, these results highlight a differential role for MyD88 deficiency on donor T-cells, with decreased GVL effect without attenuation of the GVHD severity after experimental allo-SCT.

  10. Hyperproduction of alpha-toxin by Staphylococcus aureus results in paradoxically reduced virulence in experimental endocarditis: a host defense role for platelet microbicidal proteins.

    PubMed Central

    Bayer, A S; Ramos, M D; Menzies, B E; Yeaman, M R; Shen, A J; Cheung, A L

    1997-01-01

    Staphylococcal alpha-toxin targets several cell types which are important components of cardiac vegetations in endocarditis, including platelets, erythrocytes, and endothelial cells. We evaluated the in vivo role of Staphylococcus aureus alpha-toxin in experimental endocarditis by using isogenic strains differing in the capacity to produce functional alpha-toxin, including 8325-4 (wild-type strain), DU-1090 (a mutant strain with allelic replacement of the alpha-toxin gene [hla]), DU1090(pH35L) (a mutant strain producing a target cell-binding but nonlytic toxin), DU1090(pDU1212) (a variant of DU1090 carrying the cloned hla gene on a multicopy plasmid), and DU1090(pCL84::hla) (a variant of DU1090 with a single copy of the hla gene cloned into the chromosomal lipase locus). In vitro, wild-type alpha-toxin (from parental strain 8325-4) extensively lysed both erythrocytes and platelets. In contrast, mutant alpha-toxin [from strain DU1090(pH35L)] lysed neither cell type. Following exposure to the wild-type alpha-toxin, platelet lysates were found to contain microbicidal activity against Bacillus subtilis (but not against Micrococcus luteus), as well as against the parental and alpha-toxin variant S. aureus strains noted above. Furthermore, lysate microbicidal activity was heat stable, neutralized by polyanionic filters or compounds, and recoverable from anionic filter membranes by hypertonic saline elution. These characteristics are consistent with those of cationic platelet microbicidal proteins (PMPs). Reverse-phase high-pressure liquid chromatography and polyacrylamide gel electrophoresis confirmed the presence of three distinct PMPs (1, 2, and 3) in platelet lysates. In experimental endocarditis, the two variant staphylococcal strains producing either minimal alpha-toxin or nonlytic alpha-toxin in vitro [strains DU1090 and DU1090(pH35L), respectively] exhibited significantly lower virulence in vivo than the parental strain (decreased intravegetation staphylococcal

  11. Theoretical analysis of the neuraminidase epitope of the Mexican A H1N1 influenza strain, and experimental studies on its interaction with rabbit and human hosts.

    PubMed

    Loyola, Paola Kinara Reyes; Campos-Rodríguez, R; Bello, Martiniano; Rojas-Hernández, S; Zimic, Mirko; Quiliano, Miguel; Briz, Verónica; Muñoz-Fernández, M Angeles; Tolentino-López, Luis; Correa-Basurto, Jose

    2013-05-01

    The neuraminidase (NA) epitope from the Mexican AH1N1 influenza virus was identified by using sequences registered at the GenBank during the peak of a pandemic (from April 2009 to October 2010). First, NA protein sequences were submitted for multiple alignment analysis, and their three-dimensional models (3-D) were then built by using homology modeling. The most common sequence (denominated wild-type) and its mutants were submitted to linear and nonlinear epitope predictors, which included the major histocompatibility complex type II (MHC II) and B-cell peptides. The epitope prediction was in accordance with evolutionary behavior and some protein structural properties. The latter included a low NA mutation rate, NA 3-D surface exposure, and the presence of high hindrance side chain residues. After selecting the epitope, docking studies and molecular dynamics (MD) simulations were used to explore interactions between the epitope and MHC II. Afterward, several experimental assays were performed to validate the theoretical study by using antibodies from humans (infected by pandemic H1N1) and rabbits (epitope vaccination). The results show 119 complete sequences that were grouped into 28 protein sequences according to their identity (one wild-type and 27 representative mutants (1-5 mutations)). The predictors yielded several epitopes, with the best fit being the one located in the C-terminal region. Theoretical methods demonstrated that the selected epitope reached the P4, P6, P7, and P9 pockets of MHC II, whereas the experimental evidence indicates that the epitope is recognized by human antibodies and also by rabbit antibodies immunized with the peptide.

  12. Decay of host-associated Bacteroidales cells and DNA in continuous-flow freshwater and seawater microcosms of identical experimental design and temperature as measured by PMA-qPCR and qPCR.

    PubMed

    Bae, Sungwoo; Wuertz, Stefan

    2015-03-01

    It is difficult to compare decay kinetics for genetic markers in an environmental context when they have been determined at different ambient temperatures. Therefore, we investigated the persistence of the host-associated genetic markers BacHum, BacCow and BacCan as well as the general Bacteroidales marker BacUni in both intact Bacteroidales cells and as total intracellular and extracellular marker DNA in controlled batch experiments at two temperatures using PMA-qPCR. Fecal Bacteroidales cells and DNA persisted longer at the lower temperature. Using the modified Arrhenius function to calculate decay constants for the same temperature, we then compared the decay of host-associated Bacteroidales cells and their DNA at 14 °C in field-based flow-through microcosms containing human, cow, and dog feces suspended in freshwater or seawater and previously operated with an identical experimental design. The time for a 2-log reduction (T₉₉) was used to characterize host-associated Bacteroidales decay. Host-associated genetic markers as determined by qPCR had similar T₉₉ values in freshwater and seawater at 14 °C when compared under both sunlight and dark conditions. In contrast, intact Bacteroidales cells measured by PMA-qPCR had shorter T₉₉ values in seawater than in freshwater. The decay constants of Bacteroidales cells were a function of physical (temperature) and chemical (salinity) parameters, suggesting that environmental parameters are key input variables for Bacteroidales survival in a predictive water quality model. Molecular markers targeting total Bacteroidales DNA were less susceptible to the variance of temperature, salinity and sunlight, implying that measurement of markers in both intact cells and DNA could enhance the predictive power of identifying fecal pollution across all aquatic environments. Monitoring Bacteroidales by qPCR alone rather than by PMA-qPCR does not always identify the contribution of recent fecal contamination because a

  13. Experimental Approach To Elucidating Damage Mechanisms In Cement-Well Casting-Host Rock Settings For Underground Storage of CO2

    NASA Astrophysics Data System (ADS)

    Li, C.; Jafari Azad, V.; Rodriguez, D.; Ideker, J.; Isgor, B.; Verba, C.

    2014-12-01

    During CO2 sequestration, wellbore cement could be vulnerable to high temperature and high pressure in underground storage settings. Wellbore alteration has been observed under supercritical CO2 exposure condition in previous experimental and field studies. One such study also showed that a reduction in both tensile and compressive strength when specimens were exposed to CO2-O2 gases at 50°C and 85°C as compared to specimens only exposed to CO2 gases; secondary mineral precipitation (SMP) and high temperature was speculated as the causes. In this study, experiments were designed to justify how different variables influenced SMP, and to quantify its effect on mechanical properties of Class H Portland cement. Chemical and mechanical alterations of Class H cement were investigated to verify the impact of SMP during this process. Cement prisms were investigated under different scenarios including influence of brine composition (simulated Mt. Simon basin), high temperature (up to 85 °C); high pressure (up to 4200psi); CO2 sequestration; CO2-O2 co-sequestration. Modulus of rupture and compressive strength of the cement prisms were tested under each scenario and several exposure durations. Initial results indicated that high temperature and synthetic brine composition had a negative influence on compressive strength. Pore solution analysis was conducted to examine the ionic exchange and geochemical alteration between the cement and brine. Specimens decreased in sodium and potassium, significantly increased in calcium, and slightly increased in sulfate after CO2 injected into system. Scanning electronic microscopy visually identified SMP and the alteration depth of specimens exposed to CO2 gas and CO2-O2 gases with time of exposure, respectively. The predicted degree of SMP, change of pore solution, and mechanic strength based on temperature, pressure, brine composition, and gas exposure conditions will be presented.

  14. Description of the first cryptic avian malaria parasite, Plasmodium homocircumflexum n. sp., with experimental data on its virulence and development in avian hosts and mosquitoes.

    PubMed

    Palinauskas, Vaidas; Žiegytė, Rita; Ilgūnas, Mikas; Iezhova, Tatjana A; Bernotienė, Rasa; Bolshakov, Casimir; Valkiūnas, Gediminas

    2015-01-01

    For over 100 years studies on avian haemosporidian parasite species have relied on similarities in their morphology to establish a species concept. Some exceptional cases have also included information about the life cycle and sporogonic development. More than 50 avian Plasmodium spp. have now been described. However, PCR-based studies show a much broader diversity of haemosporidian parasites, indicating the possible existence of a diverse group of cryptic species. In the present study, using both similarity and phylogenetic species definition concepts, we believe that we report the first characterised cryptic speciation case of an avian Plasmodium parasite. We used sequence information on the mitochondrial cytochrome b gene and constructed phylogenies of identified Plasmodium spp. to define their position in the phylogenetic tree. After analysis of blood stages, the morphology of the parasite was shown to be identical to Plasmodium circumflexum. However, the geographic distribution of the new parasite, the phylogenetic information, as well as patterns of development of infection, indicate that this parasite differs from P. circumflexum. Plasmodium homocircumflexum n. sp. was described based on information about genetic differences from described lineages, phylogenetic position and biological characters. This parasite develops parasitemia in experimentally infected birds - the domestic canary Serinus canaria domestica, siskin Carduelis spinus and crossbill Loxia curvirostra. Anaemia caused by high parasitemia, as well as cerebral paralysis caused by exoerythrocytic stages in the brain, are the main reasons for mortality. Exoerythrocytic stages also form in other organs (heart, kidneys, liver, lungs, spleen, intestines and pectoral muscles). DNA amplification was unsuccessful from faecal samples of heavily infected birds. The sporogonic development initiates, but is abortive, at the oocyst stage in two common European mosquito species, Culex pipiens pipiens (forms

  15. The structure of basement highs at deep-water, hyper-extended rifted margins: the example of the Briançonnais domain in the Alps and present-day examples from the Atlantic and Indian oceans

    NASA Astrophysics Data System (ADS)

    Haupert, Isabelle; Manatschal, Gianreto; Decarlis, Alessandro; Mohn, Geoffroy; Unternehr, Patrick

    2014-05-01

    in the Alps that can be compared to the structures imaged across basement highs at hyperextended domains along the Atlantic and Indian margins. This approach will enable to better define and understand the pre-Alpine 3D architecture of the Briançonnais in the Alps as well as to get insights on the structural and stratigraphic architecture of basement highs at present-day rifted margins.

  16. Assessing the volcanic hazard for Rome: 40Ar/39Ar and In-SAR constraints on the most recent eruptive activity and present-day uplift at Colli Albani Volcanic District

    NASA Astrophysics Data System (ADS)

    Marra, F.; Gaeta, M.; Giaccio, B.; Jicha, B. R.; Palladino, D. M.; Polcari, M.; Sottili, G.; Taddeucci, J.; Florindo, F.; Stramondo, S.

    2016-07-01

    We present new 40Ar/39Ar data which allow us to refine the recurrence time for the most recent eruptive activity occurred at Colli Albani Volcanic District (CAVD) and constrain its geographic area. Time elapsed since the last eruption (36 kyr) overruns the recurrence time (31 kyr) in the last 100 kyr. New interferometric synthetic aperture radar data, covering the years 1993-2010, reveal ongoing inflation with maximum uplift rates (>2 mm/yr) in the area hosting the most recent (<200 ka) vents, suggesting that the observed uplift might be caused by magma injection within the youngest plumbing system. Finally, we frame the present deformation within the structural pattern of the area of Rome, characterized by 50 m of regional uplift since 200 ka and by geologic evidence for a recent (<2000 years) switch of the local stress-field, highlighting that the precursors of a new phase of volcanic activity are likely occurring at the CAVD.

  17. An experimental study on the preparation of tochilinite-originated intercalation compounds comprised of Fe 1-xS host layers and various kinds of guest layers

    NASA Astrophysics Data System (ADS)

    Peng, Yiya; Xi, Guangcheng; Zhong, Chang; Wang, Linping; Lu, Jun; Sun, Ximeng; Zhu, Lu; Han, Qikun; Chen, Lin; Shi, Lei; Sun, Mei; Li, Qianrong; Yu, Min; Yin, Mingwen

    2009-08-01

    in composition and structure. The fourth kind of ICs was prepared by the oxidation and reduction of some of the N 2H 4-containing ICs mentioned above, which include N 2H 2 (diazene or diimide) IC, N 2 (dinitrogen) IC and NH 3 IC. The N 2H 2 IC was prepared by mild air oxidation of the N 2H 4-LiOH IC. The N 2 IC was prepared by strong air oxidation of the N 2H 4-LiOH IC, however, we have not been able to separate the pure phase N 2 IC. Hydrothermal reduction of the N 2H 4 IC made by the direct intercalation method in strong reducing environment by H 2S + Fe (metal) led to the production of the NH 3 IC of the fourth kind of ICs. The NH 3 ICs prepared by the three methods had similar compositions and structures. As almost all the ICs reported in this paper were extremely sensitive both to air and to the electron beam, they were mainly characterized by XRD. The properties and interrelationships (or mutual transformations) of the Fe 1-xS-based ICs revealed novel chemistry occurring in the sub-nanoscopic space between the micrometer- to nanometer-sized electron-deficient Fe 1-xS layers. An important finding of this novel chemistry was that the Fe 1-xS-based ICs tended to oxidize or reduce the intercalated species when the redox state of their environments varied. The results of our experiments potentially have many cosmochemical implications. The most important implication is that our experimental results, along with previous studies, strongly suggested that some of the ammonium salts, ammonia and carbonates existing in the matrix of the CM carbonaceous chondrites may have been formed by abiotic reactions employing molecular nitrogen as the nitrogen source and carbon monoxide as the carbon source and iron sulfide and/or iron hydroxide as catalysts.

  18. Sedimentological imprints of environmental variability at the Balkan Peninsula on the sediment sequence of Lake Ohrid (Macedonia, Albania) between the Mid Pleistocene Transition and present days: The ICDP SCOPSCO project

    NASA Astrophysics Data System (ADS)

    Francke, Alexander; Wagner, Bernd; Leicher, Niklas; Raphael, Gromig; Leng, Melanie; Lacey, Jack; Vogel, Hendrik; Baumgarten, Henrike; Thomas, Wonik; Zanchetta, Giovanni; Roberto, Sulpizio; Krastel, Sebastian; Lindhorst, Katja

    2015-04-01

    The UNESCO World Heritage site of Lake Ohrid in the Balkans is thought to be the oldest, continuously existing lake in Europe. In order to unravel the geological and evolutionary history of the lake, a deep drilling campaign was conducted in spring 2013 under the umbrella of the ICDP SCOPSCO project. At the coring site "DEEP" in central parts of the lake, more than 1,500 m of sediments were recovered down to a penetration depth of 569 m blf. This sediment sequence is assumed to be more than 1.2 Ma old and likely covers the entire lacustrine deposits of the Lake Ohrid Basin. Currently, an age model for the upper 260m of the DEEP- site sequence is available. This age model is based on chronological tie points (tephrochronology), and wiggle matching of down hole logging data and (bio-)geochemistry data (XRF, TIC, TOC) from the core sequence to the global benthic stack LR04 and local insolation patterns. The data suggests that the upper 260 m of the DEEP-site sequence corresponds to the time period between the Mid Pleistocene Transition (MPT) and present days. During this period, the sedimentological properties of the sediments show a strong dependency on environmental variability in the area. Interglacial deposits appear massive or marbled, contain up to 80 % of CaCO3 (high TIC), high amounts of organic matter (high TOC) and biogenic silica (high BSi), and low contents of clastic material. Glacial deposits are predominantly marbled and calcite is generally absent. Similarly, the amounts of organic matter and biogenic silica are low, and glacial sediments predominately consist of clastic matter. Distinct layers of siderite and uniformly distributed Fe- or Mn- oxides occur in the glacial deposits, vivianite concretions occur in both the glacial and interglacial periods. High CaCO3 contents in deposits formed during warm (interglacial) periods are also known from studies on short pilot cores from Lake Ohrid and are triggered by increased productivity in the lake, such as

  19. Differential Effect of MyD88 Signal in Donor T Cells on Graft-versus-Leukemia Effect and Graft-versus-Host Disease after Experimental Allogeneic Stem Cell Transplantation

    PubMed Central

    Lim, Ji-Young; Ryu, Da-Bin; Lee, Sung-Eun; Park, Gyeongsin; Choi, Eun Young; Min, Chang-Ki

    2015-01-01

    Despite the presence of toll like receptor (TLR) expression in conventional TCRαβ T cells, the direct role of TLR signaling via myeloid differentiation factor 88 (MyD88) within T lymphocytes on graft-versus-host disease (GVHD) and graft-versus-leukemia (GVL) effect after allogeneic stem cell transplantation (allo-SCT) remains unknown. In the allo-SCT model of C57BL/6 (H-2b) → B6D2F1 (H-2b/d), recipients received transplants of wild type (WT) T-cell-depleted (TCD) bone marrow (BM) and splenic T cells from either WT or MyD88 deficient (MyD88KO) donors. Host-type (H-2d) P815 mastocytoma or L1210 leukemia cells were injected either subcutaneously or intravenously to generate a GVHD/GVL model. Allogeneic recipients of MyD88KO T cells demonstrated a greater tumor growth without attenuation of GVHD severity. Moreover, GVHD-induced GVL effect, caused by increasing the conditioning intensity was also not observed in the recipients of MyD88KO T cells. In vitro, the absence of MyD88 in T cells resulted in defective cytolytic activity to tumor targets with reduced ability to produce IFN-γ or granzyme B, which are known to critical for the GVL effect. However, donor T cell expansion with effector and memory T-cell differentiation were more enhanced in GVHD hosts of MyD88KO T cells. Recipients of MyD88KO T cells experienced greater expansion of Foxp3- and IL4-expressing T cells with reduced INF-γ producing T cells in the spleen and tumor-draining lymph nodes early after transplantation. Taken together, these results highlight a differential role for MyD88 deficiency on donor T-cells, with decreased GVL effect without attenuation of the GVHD severity after experimental allo-SCT. PMID:26552489

  20. Host-to-host variation of ecological interactions in polymicrobial infections

    PubMed Central

    Mukherjee, Sayak; Weimer, Kristin E.; Seok, Sang-Cheol; Ray, Will C.; Jayaprakash, C.; Vieland, Veronica J.; Swords, W. Edward

    2014-01-01

    Host-to-host variability with respect to interactions between microorganisms and multicellular hosts are commonly observed in infection and in homeostasis. However, the majority of mechanistic models used to analyze host-microorganism relationships, as well as most of the ecological theories proposed to explain coevolution of hosts and microbes, are based on averages across a host population. By assuming that observed variations are random and independent, these models overlook the role of differences between hosts. Here, we analyze mechanisms underlying host-to-host variations of bacterial infection kinetics, using the well characterized experimental infection model of polymicrobial otitis media (OM) in chinchillas, in combination with population dynamic models and a Maximum Entropy (MaxEnt) based inference scheme. We find that the nature of the interactions between bacterial species critically regulates host-to-host variations in these interactions. Surprisingly, seemingly unrelated phenomena, such as the efficiency of individual bacterial species in utilizing nutrients for growth, and the microbe-specific host immune response, can become interdependent in a host population. The latter finding suggests a potential mechanism that could lead to selection of specific strains of bacterial species during the coevolution of the host immune response and the bacterial species. PMID:25473880

  1. Host-to-host variation of ecological interactions in polymicrobial infections

    NASA Astrophysics Data System (ADS)

    Mukherjee, Sayak; Weimer, Kristin E.; Seok, Sang-Cheol; Ray, Will C.; Jayaprakash, C.; Vieland, Veronica J.; Swords, W. Edward; Das, Jayajit

    2015-02-01

    Host-to-host variability with respect to interactions between microorganisms and multicellular hosts are commonly observed in infection and in homeostasis. However, the majority of mechanistic models used to analyze host-microorganism relationships, as well as most of the ecological theories proposed to explain coevolution of hosts and microbes, are based on averages across a host population. By assuming that observed variations are random and independent, these models overlook the role of differences between hosts. Here, we analyze mechanisms underlying host-to-host variations of bacterial infection kinetics, using the well characterized experimental infection model of polymicrobial otitis media (OM) in chinchillas, in combination with population dynamic models and a maximum entropy (MaxEnt) based inference scheme. We find that the nature of the interactions between bacterial species critically regulates host-to-host variations in these interactions. Surprisingly, seemingly unrelated phenomena, such as the efficiency of individual bacterial species in utilizing nutrients for growth, and the microbe-specific host immune response, can become interdependent in a host population. The latter finding suggests a potential mechanism that could lead to selection of specific strains of bacterial species during the coevolution of the host immune response and the bacterial species.

  2. Host-to-host variation of ecological interactions in polymicrobial infections.

    PubMed

    Mukherjee, Sayak; Weimer, Kristin E; Seok, Sang-Cheol; Ray, Will C; Jayaprakash, C; Vieland, Veronica J; Swords, W Edward; Das, Jayajit

    2014-01-01

    Host-to-host variability with respect to interactions between microorganisms and multicellular hosts are commonly observed in infection and in homeostasis. However, the majority of mechanistic models used to analyze host-microorganism relationships, as well as most of the ecological theories proposed to explain coevolution of hosts and microbes, are based on averages across a host population. By assuming that observed variations are random and independent, these models overlook the role of differences between hosts. Here, we analyze mechanisms underlying host-to-host variations of bacterial infection kinetics, using the well characterized experimental infection model of polymicrobial otitis media (OM) in chinchillas, in combination with population dynamic models and a maximum entropy (MaxEnt) based inference scheme. We find that the nature of the interactions between bacterial species critically regulates host-to-host variations in these interactions. Surprisingly, seemingly unrelated phenomena, such as the efficiency of individual bacterial species in utilizing nutrients for growth, and the microbe-specific host immune response, can become interdependent in a host population. The latter finding suggests a potential mechanism that could lead to selection of specific strains of bacterial species during the coevolution of the host immune response and the bacterial species. PMID:25473880

  3. [Situation of sultopride among present-day neuroleptics].

    PubMed

    Maurel, H; Pujol, B

    1975-01-01

    The authors report preliminary clinical investigations about sultopride, a new substituted benzamid, related to sulpiride. The drug was administered to thirty-nine hospitalized psychotic patients. A very powerful and constant efficacy of sultopride was observed in 11 manic and hypomanic typical syndroms: excitation was controlled between the first and third day, with oral doses of 1,200-1,800 mg. But thymical inversion was observed in 8 cases (3 light anxious states and 5 typical melancholic syndroms). Besides, interesting improvements were obtained in atypical excitation disorders, chronic hallucinatory delusions, acute oniric and confusional states, schizophrenia and chronic alcoholism. Side-effects were frequent: extrapyramidal syndroms, often requiring antiparkinsonian correctors, somnolence, asthenia, and above all (in 30 percent of cases) psychical side-effects, consisting in depressive and anxious modifications of mood, even apart from manic-melancholic psychosis. This psychotropic depressive effect appears as very interesting theoretically, and justifies further enquiry.

  4. Earthquakes and present-day tectonism in Iceland

    NASA Astrophysics Data System (ADS)

    Einarsson, Páll

    1991-04-01

    The mid-Atlantic plate boundary in Iceland is expressed by a series of seismic and volcanic zones. The structure of the plate boundary is strongly influenced by the Iceland hot spot. The relative motion of the Mid-Atlantic Ridge with respect to the hot spot leads to ridge jumps, propagating rifts and other complexities. Most large earthquakes in Iceland occur within two transform zones that connect the presently active Northern and Eastern Volcanic Zones to the ridges offshore. In the south the South Iceland Seismic Zone is marked by a 10-15 km wide, E-trending epicentral belt. The large earthquakes occur by faulting on N-S striking right-lateral faults. The left-lateral transform motion along the zone thus appears to be taken up by slip on numerous parallel faults by counterclockwise rotation of the blocks between them (bookshelf tectonics). It is argued that the South Iceland Seismic Zone is a transient feature, migrating sideways in response to propagation of the Eastern Volcanic Zone. In Northern Iceland the transform motion is taken up along the Tjörnes Fracture Zone. At least three parallel, NW-trending seismic belts have been identified within the zone. The seismicity of the volcanic zones is characterized by spatial clustering of epicenters. Most clusters coincide with central volcanoes. Rifting structures such as fissure swarms and normal faults are mostly aseismic except during episodes of rifting and magmatism such as the present events in Krafla. Earthquake recording has been used very successfully at Krrafla to monitor the level of inflation and deflation of the volcano, and to trace the path and speed of lateral magmatic intrusions into the associated fissure swarm. Seismic activity at the Bárdarbunga volcano in Central Iceland correlates in time with the Krafla events, and it seems as if inflation of Krafla is followed by deflation of Bárdarbunga. It is postulated that the pressure drop in the partially molten mantle beneath Krafla is transmitted to neighboring volcanoes, leading to magma withdrawal from their shallow reservoirs. Bursts of seismicity of the Katla volcano in South Iceland in 1967 and 1977 may similarly be the result of magma withdrawal in response to the 1964-1967 Surtsey and 1973 Heimaey eruptions. Annual periodicity seen in the Katla seismicity is explained as the result of the triggering effect of pore pressure in the crust beneath the glacier covering Katla. Several volcanoes exhibit persistent, low-magnitude seismicity. In the Hengill volcano in Southwest Iceland, many events involve a non-double-couple mechanism. The seismicity is interpreted as the result of extensional failure and heat extraction from a cooling magma chamber. Two classes of intraplate earthquakes have been identified in Iceland. One includes events in the lithospheric blockbetween the transform zones. These events are related to crustal extension above the hot spot. The other class includes events on the insular shelf off the east and southeast coasts which are possibly caused by a differential cooling rate in the crust across the shelf edge.

  5. Teaching with Social Media: Disrupting Present Day Public Education

    ERIC Educational Resources Information Center

    Meabon Bartow, Susan

    2014-01-01

    Because social technologies present illuminating educational, ethical, economic, and structural challenges to existing constructions of public education, they catalyze a fundamental examination of what public education should look like and be like in a democracy. Given their performances in other arenas, mobile and electronic technologies have the…

  6. The ethical debate on present day paternity testing practices.

    PubMed

    Mertens, G

    2006-01-01

    The last years, the number of paternity tests on buccal swabs sold over the internet as "test kits", has steeply increased. The commercial providers of these services facilitate controversial practices, including clandestine sampling at home, anonymous sending off for analysis, motherless testing and using "stolen" personal objects containing biological material (combs, cigarette butts). This has led to concern on the consequences on the family unit--especially the child--which may suffer emotionally, physically and financially. In reaction, legal initiatives are appearing throughout Europe. The UK Human Genetics Commission has advised that the non-consensual obtaining and analysis of personal genetic information should be a new criminal offence. The German Federal Court of Justice has ruled that paternity tests performed without the mother's knowledge are inadmissible as evidence in lawsuits. French law strictly forbids the application of DNA testing without the involvement of the court system. In Belgium, a proposal for law has been laid down where the offering to PMID:16792338

  7. Human Capital and Its Development in Present-Day Russia

    ERIC Educational Resources Information Center

    Nureev, R. M.

    2010-01-01

    In the broad sense of the word human capital is a specific form of capital that is embodied in people themselves. It consists of the individual's reserve of health, knowledge, skills, abilities, and motivations that enable him to increase his labor productivity and give him an income in the form of wages, salaries, and other income. The structure…

  8. Present day serpentinization in New Caledonia, Oman and Yugoslavia

    USGS Publications Warehouse

    Barnes, I.; O'Neil, J.R.; Trescases, J.J.

    1978-01-01

    Geochemical evidence for modern low-temperature serpentinization has been found in three new localities. Apparently the low-temperature reactions are a common mode of formation of the lizardite-chrysotile and brucite assemblage. Possibly the 18O content of serpentine formed at low temperatures is in part inherited from the pyroxene and olivine. ?? 1978.

  9. Syphilis: An Old Disease With Present-Day Implications.

    PubMed

    McNeil, Candice J; Bachmann, Laura H

    2016-01-01

    Syphilis continues to be a burden on the public health system. While men who have sex with men and HIV-infected individuals are the most affected populations, syphilis rates have also increased in reproductive-aged women, resulting in concurrent increases in congenital syphilis. Prompt diagnosis and treatment are requisite components of syphilis control. PMID:27621352

  10. [Present-day metal-cutting tools and working conditions].

    PubMed

    Kondratiuk, V P

    1990-01-01

    Polyfunctional machine-tools of a processing centre type are characterized by a set of hygienic advantages as compared to universal machine-tools. But low degree of mechanization and automation of some auxiliary processes, and constructional defects which decrease the ergonomic characteristics of the tools, involve labour intensity in multi-machine processing. The article specifies techniques of allowable noise level assessment, and proposes hygienic recommendations, some of which have been introduced into practice.

  11. Syphilis: An Old Disease With Present-Day Implications.

    PubMed

    McNeil, Candice J; Bachmann, Laura H

    2016-01-01

    Syphilis continues to be a burden on the public health system. While men who have sex with men and HIV-infected individuals are the most affected populations, syphilis rates have also increased in reproductive-aged women, resulting in concurrent increases in congenital syphilis. Prompt diagnosis and treatment are requisite components of syphilis control.

  12. Catalogues of variable stars from Parenago to the present day

    NASA Astrophysics Data System (ADS)

    Samus, N. N.

    2006-04-01

    After World War II, the International Astronomical Union made Soviet astronomers responsible for variable-star catalogues. This work has been continued ever since the first edition of the General Catalogue of Variable Stars compiled by the team headed by P.P. Parenago and B.V. Kukarkin and published in 1948. Currently, the catalogue work is a joint project of the Institute of Astronomy (Russian Academy of Sciences) and the Sternberg Astronomical Institute (Moscow University). This paper is a brief review of recent trends in the field of variable-star catalogues. Problems as well as new prospects related to modern large-scale automatic photometric sky surveys are discussed.

  13. [Fire worship on Soratte: form prehistory to present days].

    PubMed

    Falchetti, Mario; Ottini, Laura

    2013-01-01

    Fire has always been involved in human thoughts, believes and actions. For many psychological reasons fire has been considered as living power: red as the human blood, warm as the human body, clearly shines in the night. Fire worship is very common, especially in regions where terrestrial fire is believed to be the image of the heavenly fire. Fire worship has represented the base for symbolic systems involved in the cultural-anthropological evolution of populations settled close to mount Soratte. Fire cults in this region have originated in independent and long-time separated contexts, according to available knowledge. Hirpi Sorani, ancient inhabitants of mount Soratte territory, celebrated Sorano Apollo by a famous fire walking ceremony, with a likely cathartic and apotropaic meaning, as reported by ancient Roman writers. The victory of Christianity over paganism caused the decline of these religious practices. The beginning of the XIX century witnessed the establishment ofa new kind of devotion to the Virgin Mary at the mount Soratte. Today, this religious piety-linked identity has weakened due to changes in lifestyles,from an agricultural to one based on outside home employment, and has been largely shifted on cultural and anthropological bases. In conclusion, fire worship at mount Soratte has evolved because of external influences and local inhabitants have reacted by asserting their own identity. PMID:25807785

  14. Present-day uplift of the western Alps

    PubMed Central

    Nocquet, J.-M.; Sue, C.; Walpersdorf, A.; Tran, T.; Lenôtre, N.; Vernant, P.; Cushing, M.; Jouanne, F.; Masson, F.; Baize, S.; Chéry, J.; van der Beek, P. A.

    2016-01-01

    Collisional mountain belts grow as a consequence of continental plate convergence and eventually disappear under the combined effects of gravitational collapse and erosion. Using a decade of GPS data, we show that the western Alps are currently characterized by zero horizontal velocity boundary conditions, offering the opportunity to investigate orogen evolution at the time of cessation of plate convergence. We find no significant horizontal motion within the belt, but GPS and levelling measurements independently show a regional pattern of uplift reaching ~2.5 mm/yr in the northwestern Alps. Unless a low viscosity crustal root under the northwestern Alps locally enhances the vertical response to surface unloading, the summed effects of isostatic responses to erosion and glaciation explain at most 60% of the observed uplift rates. Rock-uplift rates corrected from transient glacial isostatic adjustment contributions likely exceed erosion rates in the northwestern Alps. In the absence of active convergence, the observed surface uplift must result from deep-seated processes. PMID:27346228

  15. Present-day uplift of the western Alps.

    PubMed

    Nocquet, J-M; Sue, C; Walpersdorf, A; Tran, T; Lenôtre, N; Vernant, P; Cushing, M; Jouanne, F; Masson, F; Baize, S; Chéry, J; van der Beek, P A

    2016-06-27

    Collisional mountain belts grow as a consequence of continental plate convergence and eventually disappear under the combined effects of gravitational collapse and erosion. Using a decade of GPS data, we show that the western Alps are currently characterized by zero horizontal velocity boundary conditions, offering the opportunity to investigate orogen evolution at the time of cessation of plate convergence. We find no significant horizontal motion within the belt, but GPS and levelling measurements independently show a regional pattern of uplift reaching ~2.5 mm/yr in the northwestern Alps. Unless a low viscosity crustal root under the northwestern Alps locally enhances the vertical response to surface unloading, the summed effects of isostatic responses to erosion and glaciation explain at most 60% of the observed uplift rates. Rock-uplift rates corrected from transient glacial isostatic adjustment contributions likely exceed erosion rates in the northwestern Alps. In the absence of active convergence, the observed surface uplift must result from deep-seated processes.

  16. Present-day aeolian activity in Herschel Crater, Mars

    NASA Astrophysics Data System (ADS)

    Cardinale, Marco; Silvestro, Simone; Vaz, David A.; Michaels, Timothy; Bourke, Mary C.; Komatsu, Goro; Marinangeli, Lucia

    2016-02-01

    In this report, we show evidence for ripple and dune migration in Herschel Crater on Mars. We estimate an average dune migration of 0.8 m and a minimum ripple migration of 1.1 m in a time span of 3.7 Earth-years. These dunes and ripples are mainly shaped by prevailing winds coming from the north, however we also report the presence of secondary winds which elongate the barchans' horns. Such a complex wind scenario is likely caused by the influence of winds blowing off the western crater rim as suggested by the Mars Regional Atmospheric Modeling System (MRAMS), an atmospheric mesoscale model. A multi-directional wind regime at the local scale is also supported by the observed bimodal distribution of the ripple trends. For the first time, a survey integrating the assessment of dune and ripple migration is presented, showing how dune topography can influence the migration patterns of ripples and how underlying topography appears to control the rates of dune migration.

  17. Present-day uplift of the western Alps

    NASA Astrophysics Data System (ADS)

    Nocquet, J.-M.; Sue, C.; Walpersdorf, A.; Tran, T.; Lenôtre, N.; Vernant, P.; Cushing, M.; Jouanne, F.; Masson, F.; Baize, S.; Chéry, J.; van der Beek, P. A.

    2016-06-01

    Collisional mountain belts grow as a consequence of continental plate convergence and eventually disappear under the combined effects of gravitational collapse and erosion. Using a decade of GPS data, we show that the western Alps are currently characterized by zero horizontal velocity boundary conditions, offering the opportunity to investigate orogen evolution at the time of cessation of plate convergence. We find no significant horizontal motion within the belt, but GPS and levelling measurements independently show a regional pattern of uplift reaching ~2.5 mm/yr in the northwestern Alps. Unless a low viscosity crustal root under the northwestern Alps locally enhances the vertical response to surface unloading, the summed effects of isostatic responses to erosion and glaciation explain at most 60% of the observed uplift rates. Rock-uplift rates corrected from transient glacial isostatic adjustment contributions likely exceed erosion rates in the northwestern Alps. In the absence of active convergence, the observed surface uplift must result from deep-seated processes.

  18. Present-day anthelmintics and perspectives on future new targets.

    PubMed

    Taman, Amira; Azab, Manar

    2014-07-01

    In absence of vaccines for the majority of helminths, chemotherapy is still the mainstay for controlling human helminthiases. However, a limited number of drugs are available in the market to combat parasitic helminths in human. Besides, the development and spread of drug resistance have declined the use of most currently available anthelmintics. Clearly, availability of new anthelmintic agents will be essential in the next few years. More research into the mechanisms of drug actions and their targets are eminent for the discovery and development of novel anthelmintic agents. Recent drug discovery techniques mostly rely on mechanism-based screening of compounds on heterologously expressed targets in bacterial, mammalian or yeast cells. Although this is usually a successful approach, it is money- and time-consuming; meanwhile, pharmaceutical companies prefer the tested target that is chosen based on basic research. The nervous system is the site of action of several chemotherapeutics including pesticides and antinematode drugs; accordingly, the nervous system continues to be a promising target. Recent advances in exploring helminths' nervous system, neurotransmitters and receptors have paved the way for the development of potential agents targeting the nervous system and its components.

  19. Tracing galaxy evolution by their present-day luminosity function

    NASA Astrophysics Data System (ADS)

    Tempel, Elmo

    2011-04-01

    Galaxies, which are complex objects containing up to several tens of billions stars, as well as gas and dust, are remarkable objects. The Universe contains a very diverse "zoo" of galaxies: there are galaxies with a discy shape and spiral structure, elliptical galaxies, and even galaxies, which show no sign of structure. This variety of galaxies leads to the basic question: how the galaxies form and evolve and which processes shape the structure of galaxies? Due to the complexity of galaxy formation and evolution, this question is still an unresolved puzzle and it is one of the biggest challenges in modern cosmology. The present thesis is based on large galaxy surveys and concentrates on the large-scale structure: how galaxy evolution is related to the surrounding large-scale environment of superclusters and voids. To study the evolution of galaxies, we use the luminosity function, which is in this respect one of the most fundamental of all cosmological observables. One of the principal results of the present study was the conclusion that the evolution of spiral galaxies is almost independent of the global environment, especially for blue and red spirals separately, showing that the formation of spiral galaxies has to be similar in all environments. Meanwhile, the luminosity function of elliptical galaxies depends strongly on the environment. This shows that the global environmental density is an important factor (via merging history) in the formation of elliptical galaxies. The results of the present study show clearly, that besides the local/group environment, the global (supercluster-void) environment plays also an important role in the formation and evolution of galaxies. Accounting for the role of global environment can help to solve several problems in the present picture of galaxy formation and evolution.

  20. Illiteracy, Sex and Occupational Status in Present-Day China.

    ERIC Educational Resources Information Center

    Lamontagne, Jacques

    This study determined the magnitude of disparity between men and women in China in relation to illiteracy and occupational status. Region and ethnicity are used as control variables. The data collected are from a 10 percent sampling of the 1982 census; the total sample size includes a population of 100,380,000 nationwide. The census questionnaire…

  1. Likelihood of nitrogen condensation in Titan's present-day atmosphere

    NASA Astrophysics Data System (ADS)

    Tokano, Tetsuya

    2016-04-01

    The temperature in Titan's upper troposphere measured by the Huygens Probe is relatively close to the nitrogen condensation point. This poses the question as to whether seasonal nitrogen condensation might occur on Titan analogously to seasonal carbon dioxide condensation on Mars. The likelihood of nitrogen condensation in Titan's atmosphere is investigated using tropospheric temperature data obtained by Cassini radio occultations and simulation with a general circulation model (GCM). The observed tropospheric temperature generally decreases towards both poles but does not reach the nitrogen condensation point anywhere. However, Cassini may not have sounded the coldest season and area in Titan's troposphere. The GCM simulation shows that in the upper troposphere the variable solar distance associated with Saturn's orbital eccentricity has a larger impact on the seasonal polar temperature variation than the variable solar declination associated with Saturn's obliquity. In the upper troposphere relevant for nitrogen condensation the annual minimum polar temperature is predicted to occur around the northern autumnal equinox, approximately one season after aphelion. This temperature is then 1-2 K lower than in the season of the Cassini/Huygens mission. It is possible if not certain that some nitrogen condensation with cloud formation occurs in the northern and southern polar region in the upper troposphere around the northern autumnal equinox. Under the present orbital parameters of Saturn and Titan nitrogen condensation may occur more frequently near the south pole than near the north pole.

  2. Active Immunization—Some Present-Day Problems

    PubMed Central

    1941-01-01

    Diphtheria.—Immunization is safe and effective. Compulsory measures are indicated, especially for the younger age-groups. The Schick test may be reserved for selected groups and to control modified methods. Proper spacing of doses of prophylactics and periodic reinoculation will ensure a high level of immunity. It is important to beware of “one-shot” methods. Indiscriminate swabbing is to be discouraged; high carrier rates are an indication for widespread diphtheria prophylaxis. Enteric fever.—Mass immunization is desirable in many areas, although there is no justification for compulsion except for people exposed to special risks. In deciding upon dosage of vaccine, more attention should be paid to physical state and body-weight. After the primary course, very small periodic doses (for example o.i.c.c) are worthy of trial. Vaccine can be given during an epidemic without increasing the chances of infection. Tetanus.—Two doses of toxoid spaced by six weeks give useful immunity. Women give significantly higher titres than men. A third dose of i.o.c.c. after a long interval—seven to nine months—often produces a dramatic rise in circulating antitoxin, and counteracts any tendency to waning immunity. Smallpox.—As vaccination has not been made compulsory in this country, infection by virulent strains from the continent may tax the resources of the public health services. Whooping-cough.—The large number of injections of vaccine usually recommended is a deterrent to mass immunization. Research into the possibility of fewer doses and wider spacing is indicated. Other diseases are considered briefly. Combined immunization.—It may be useful to combine diphtheria T.A.F. and tetanus toxoid, also tetanus toxoid and T.A.B. vaccine. T.A.F. plus T.A.B. is probably contra-indicated for adults on account of severe reactions. Diphtheria A.P.T. should not be mixed with tetanus toxoid as it may go into solution and become ineffective. Sterilization of syringes and needles.—An intensive inoculation campaign is no excuse for slip-shod methods. PMID:19992328

  3. Microplate model for the present-day deformation of Tibet

    USGS Publications Warehouse

    Thatcher, W.

    2007-01-01

    Site velocities from 349 Global Positioning System (GPS) stations are used to construct an 11-element quasi-rigid block model of the Tibetan Plateau and its surroundings. Rigid rotations of five major blocks are well determined, and average translation velocities of six smaller blocks can be constrained. Where data are well distributed the velocity field can be explained well by rigid block motion and fault slip across block boundaries. Residual misfits average 1.6 mm/yr compared to typical one standard deviation velocity uncertainties of 1.3 mm/yr. Any residual internal straining of the blocks is small and heterogeneous. However, residual substructure might well represent currently unresolved motions of smaller blocks. Although any smaller blocks must move at nearly the same rate as the larger blocks within which they lie, undetected relative motions between them could be significant, particularly where there are gaps in GPS coverage. Predicted relative motions between major blocks agree with the observed sense of slip and along-strike partitioning of motion across major faults. However, predicted slip rates across Tibet's major strike-slip faults are low, only 5-12 mm/yr, a factor of 2-3 smaller than most rates estimated from fault offset features dated by radiometric methods as ???2000 to ???100,000 year old. Previous work has suggested that both GPS data and low fault slip rates are incompatible with rigid block motions of Tibet. The results reported here overcome these objections.

  4. No adaptation of a herbivore to a novel host but loss of adaptation to its native host.

    PubMed

    Grosman, Amir H; Molina-Rugama, Adrián J; Mendes-Dias, Rondinelli; Sabelis, Maurice W; Menken, Steph B J; Pallini, Angelo; Breeuwer, Johannes A J; Janssen, Arne

    2015-01-01

    Most herbivorous arthropods are host specialists and the question is which mechanisms drive the evolution of such specialization. The theory of antagonistic pleiotropy suggests that there is a trade-off between adaptation of herbivores to a novel host and their native host. The mutation accumulation hypothesis proposes that herbivores on a novel host lose their adaptation to the native host through the accumulation of mutations with negligible effects on performance on the novel host. Experimental evidence for either of the two hypotheses is scarce. We compared the fitness of two sympatric moth strains from an introduced host and a native host. The strain from the novel host did not perform better on this host than the strain from the native host. The strain from the novel host performed less well on the native host than did the strain from the native host. Hence, selection on the novel host did not result in noticeable gain in performance, but adaptation to the native host was lost. These results are more readily explained by the mutation-accumulation hypothesis than by the trade-off hypothesis. PMID:26577696

  5. No adaptation of a herbivore to a novel host but loss of adaptation to its native host

    PubMed Central

    Grosman, Amir H.; Molina-Rugama, Adrián J.; Mendes-Dias, Rondinelli; Sabelis, Maurice W.; Menken, Steph B.J.; Pallini, Angelo; Breeuwer, Johannes A.J.; Janssen, Arne

    2015-01-01

    Most herbivorous arthropods are host specialists and the question is which mechanisms drive the evolution of such specialization. The theory of antagonistic pleiotropy suggests that there is a trade-off between adaptation of herbivores to a novel host and their native host. The mutation accumulation hypothesis proposes that herbivores on a novel host lose their adaptation to the native host through the accumulation of mutations with negligible effects on performance on the novel host. Experimental evidence for either of the two hypotheses is scarce. We compared the fitness of two sympatric moth strains from an introduced host and a native host. The strain from the novel host did not perform better on this host than the strain from the native host. The strain from the novel host performed less well on the native host than did the strain from the native host. Hence, selection on the novel host did not result in noticeable gain in performance, but adaptation to the native host was lost. These results are more readily explained by the mutation-accumulation hypothesis than by the trade-off hypothesis. PMID:26577696

  6. Glob-Hosts

    SciTech Connect

    Behlendorf, B.; Garlick, J.

    2007-08-31

    The glob-hosts utility manipulates hostlist strings in UNIX shell scripts. Hostlists are a parseable string representatin of list of hosts, which compress nicely when a group of hosts are named with contiguous numeric suffixes. For example, the hosts blue1, blue2, and blue3 can be represented by the hostlist string "blue1, blue2, blue3" or equivalently "blue[1-3]". The globhost utility cn peform the following operations on a hostlist string: count, size, expand, nth, union, minus, intersection, and exclude.

  7. Glob-Hosts

    2007-08-31

    The glob-hosts utility manipulates hostlist strings in UNIX shell scripts. Hostlists are a parseable string representatin of list of hosts, which compress nicely when a group of hosts are named with contiguous numeric suffixes. For example, the hosts blue1, blue2, and blue3 can be represented by the hostlist string "blue1, blue2, blue3" or equivalently "blue[1-3]". The globhost utility cn peform the following operations on a hostlist string: count, size, expand, nth, union, minus, intersection, andmore » exclude.« less

  8. Host-Pathogen Interactions in Campylobacter Infections: the Host Perspective

    PubMed Central

    Janssen, Riny; Krogfelt, Karen A.; Cawthraw, Shaun A.; van Pelt, Wilfrid; Wagenaar, Jaap A.; Owen, Robert J.

    2008-01-01

    Campylobacter is a major cause of acute bacterial diarrhea in humans worldwide. This study was aimed at summarizing the current understanding of host mechanisms involved in the defense against Campylobacter by evaluating data available from three sources: (i) epidemiological observations, (ii) observations of patients, and (iii) experimental observations including observations of animal models and human volunteer studies. Analysis of available data clearly indicates that an effective immune system is crucial for the host defense against Campylobacter infection. Innate, cell-mediated, and humoral immune responses are induced during Campylobacter infection, but the relative importance of these mechanisms in conferring protective immunity against reinfection is unclear. Frequent exposure to Campylobacter does lead to the induction of short-term protection against disease but most probably not against colonization. Recent progress in the development of more suitable animal models for studying Campylobacter infection has opened up possibilities to study the importance of innate and adaptive immunity during infection and in protection against reinfection. In addition, advances in genomics and proteomics technologies will enable more detailed molecular studies. Such studies combined with better integration of host and pathogen research driven by epidemiological findings may truly advance our understanding of Campylobacter infection in humans. PMID:18625685

  9. Inter- and intraspecific conflicts between parasites over host manipulation

    PubMed Central

    Hafer, Nina; Milinski, Manfred

    2016-01-01

    Host manipulation is a common strategy by which parasites alter the behaviour of their host to enhance their own fitness. In nature, hosts are usually infected by multiple parasites. This can result in a conflict over host manipulation. Studies of such a conflict in experimentally infected hosts are rare. The cestode Schistocephalus solidus (S) and the nematode Camallanus lacustris (C) use copepods as their first intermediate host. They need to grow for some time inside this host before they are infective and ready to be trophically transmitted to their subsequent fish host. Accordingly, not yet infective parasites manipulate to suppress predation. Infective ones manipulate to enhance predation. We experimentally infected laboratory-bred copepods in a manner that resulted in copepods harbouring (i) an infective C plus a not yet infective C or S, or (ii) an infective S plus a not yet infective C. An infective C completely sabotaged host manipulation by any not yet infective parasite. An infective S partially reduced host manipulation by a not yet infective C. We hence show experimentally that a parasite can reduce or even sabotage host manipulation exerted by a parasite from a different species. PMID:26842574

  10. COMPARISON OF IN VITRO-CULTURED AND WILD-TYPE PERKINSUS MARINUS. II: DOSING METHODS AND HOST RESPONSE

    EPA Science Inventory

    Endoparasites must breach host barriers to establish infection and then must survive host internal defenses to cause disease. Such barriers may frustrate attempts to experimentally transmit parasites by ?natural' methods. In addition, the host's condition may affect a study's out...

  11. Standardised animal models of host microbial mutualism

    PubMed Central

    Macpherson, A J; McCoy, K D

    2015-01-01

    An appreciation of the importance of interactions between microbes and multicellular organisms is currently driving research in biology and biomedicine. Many human diseases involve interactions between the host and the microbiota, so investigating the mechanisms involved is important for human health. Although microbial ecology measurements capture considerable diversity of the communities between individuals, this diversity is highly problematic for reproducible experimental animal models that seek to establish the mechanistic basis for interactions within the overall host-microbial superorganism. Conflicting experimental results may be explained away through unknown differences in the microbiota composition between vivaria or between the microenvironment of different isolated cages. In this position paper, we propose standardised criteria for stabilised and defined experimental animal microbiotas to generate reproducible models of human disease that are suitable for systematic experimentation and are reproducible across different institutions. PMID:25492472

  12. Poxviruses and the Evolution of Host Range and Virulence

    PubMed Central

    Haller, Sherry L.; Peng, Chen; McFadden, Grant; Rothenburg, Stefan

    2013-01-01

    Poxviruses as a group can infect a large number of animals. However, at the level of individual viruses, even closely related poxviruses display highly diverse host ranges and virulence. For example, variola virus, the causative agent of smallpox, is human-specific and highly virulent only to humans, whereas related cowpox viruses naturally infect a broad spectrum of animals and only cause relatively mild disease in humans. The successful replication of poxviruses depends on their effective manipulation of the host antiviral responses, at the cellular-, tissue- and species-specific levels, which constitutes a molecular basis for differences in poxvirus host range and virulence. A number of poxvirus genes have been identified that possess host range function in experimental settings, and many of these host range genes target specific antiviral host pathways. Herein, we review the biology of poxviruses with a focus on host range, zoonotic infections, virulence, genomics and host range genes as well as the current knowledge about the function of poxvirus host range factors and how their interaction with the host innate immune system contributes to poxvirus host range and virulence. We further discuss the evolution of host range and virulence in poxviruses as well as host switches and potential poxvirus threats for human and animal health. PMID:24161410

  13. Asteroseismology and Exoplanet Hosts

    NASA Astrophysics Data System (ADS)

    Huber, Daniel

    2015-08-01

    Asteroseismology is among the most powerful observational tools to determine fundamental properties of stars. Space-based photometry has recently enabled the systematic detection of oscillations in exoplanet host stars, allowing a combination of asteroseismology with transit and radial-velocity measurements to precisely characterize planetary systems. In this talk I will review the latest asteroseismic detections in exoplanet host stars spanning from the main sequence to the red-giant branch, focusing in particular on radii and ages of stars hosting small (sub-Neptune sized) planets discovered by the Kepler mission. I will furthermore discuss applications of asteroseismology to measure spin-orbit inclinations in multiplanet systems, and their implications for formation theories of hot Jupiters. Finally I will give an outlook on asteroseismic studies of exoplanet hosts with current and future space- and ground-based facilities such as K2, SONG, TESS, and PLATO.

  14. Experimental Models of Cryptococcosis

    PubMed Central

    Sabiiti, Wilber; May, Robin C.; Pursall, E. Rhiannon

    2012-01-01

    Cryptococcosis is a life-threatening fungal disease that infects around one million people each year. Establishment and progression of disease involves a complex interplay between the fungus and a diverse range of host cell types. Over recent years, numerous cellular, tissue, and animal models have been exploited to probe this host-pathogen interaction. Here we review the range of experimental models that are available for cryptococcosis research and compare the relative advantages and limitations of the different systems. PMID:22007224

  15. Association and Host Selectivity in Multi-Host Pathogens

    PubMed Central

    Malpica, José M.; Sacristán, Soledad; Fraile, Aurora; García-Arenal, Fernando

    2006-01-01

    The distribution of multi-host pathogens over their host range conditions their population dynamics and structure. Also, host co-infection by different pathogens may have important consequences for the evolution of hosts and pathogens, and host-pathogen co-evolution. Hence it is of interest to know if the distribution of pathogens over their host range is random, or if there are associations between hosts and pathogens, or between pathogens sharing a host. To analyse these issues we propose indices for the observed patterns of host infection by pathogens, and for the observed patterns of co-infection, and tests to analyse if these patterns conform to randomness or reflect associations. Applying these tests to the prevalence of five plant viruses on 21 wild plant species evidenced host-virus associations: most hosts and viruses were selective for viruses and hosts, respectively. Interestingly, the more host-selective viruses were the more prevalent ones, suggesting that host specialisation is a successful strategy for multi-host pathogens. Analyses also showed that viruses tended to associate positively in co-infected hosts. The developed indices and tests provide the tools to analyse how strong and common are these associations among different groups of pathogens, which will help to understand and model the population biology of multi-host pathogens. PMID:17183670

  16. Parasite transmission in social interacting hosts: Monogenean epidemics in guppies

    USGS Publications Warehouse

    Johnson, M.B.; Lafferty, K.D.; van, Oosterhout C.; Cable, J.

    2011-01-01

    Background: Infection incidence increases with the average number of contacts between susceptible and infected individuals. Contact rates are normally assumed to increase linearly with host density. However, social species seek out each other at low density and saturate their contact rates at high densities. Although predicting epidemic behaviour requires knowing how contact rates scale with host density, few empirical studies have investigated the effect of host density. Also, most theory assumes each host has an equal probability of transmitting parasites, even though individual parasite load and infection duration can vary. To our knowledge, the relative importance of characteristics of the primary infected host vs. the susceptible population has never been tested experimentally. Methodology/Principal Findings: Here, we examine epidemics using a common ectoparasite, Gyrodactylus turnbulli infecting its guppy host (Poecilia reticulata). Hosts were maintained at different densities (3, 6, 12 and 24 fish in 40 L aquaria), and we monitored gyrodactylids both at a population and individual host level. Although parasite population size increased with host density, the probability of an epidemic did not. Epidemics were more likely when the primary infected fish had a high mean intensity and duration of infection. Epidemics only occurred if the primary infected host experienced more than 23 worm days. Female guppies contracted infections sooner than males, probably because females have a higher propensity for shoaling. Conclusions/Significance: These findings suggest that in social hosts like guppies, the frequency of social contact largely governs disease epidemics independent of host density. ?? 2011 Johnson et al.

  17. Parasite transmission in social interacting hosts: Monogenean epidemics in guppies

    USGS Publications Warehouse

    Johnson, Mirelle B.; Lafferty, Kevin D.; van Oosterhout, Cock; Cable, Joanne

    2011-01-01

    Background Infection incidence increases with the average number of contacts between susceptible and infected individuals. Contact rates are normally assumed to increase linearly with host density. However, social species seek out each other at low density and saturate their contact rates at high densities. Although predicting epidemic behaviour requires knowing how contact rates scale with host density, few empirical studies have investigated the effect of host density. Also, most theory assumes each host has an equal probability of transmitting parasites, even though individual parasite load and infection duration can vary. To our knowledge, the relative importance of characteristics of the primary infected host vs. the susceptible population has never been tested experimentally. Methodology/Principal Findings Here, we examine epidemics using a common ectoparasite, Gyrodactylus turnbulli infecting its guppy host (Poecilia reticulata). Hosts were maintained at different densities (3, 6, 12 and 24 fish in 40 L aquaria), and we monitored gyrodactylids both at a population and individual host level. Although parasite population size increased with host density, the probability of an epidemic did not. Epidemics were more likely when the primary infected fish had a high mean intensity and duration of infection. Epidemics only occurred if the primary infected host experienced more than 23 worm days. Female guppies contracted infections sooner than males, probably because females have a higher propensity for shoaling. Conclusions/Significance These findings suggest that in social hosts like guppies, the frequency of social contact largely governs disease epidemics independent of host density.

  18. Host Responses to Biofilm.

    PubMed

    Watters, C; Fleming, D; Bishop, D; Rumbaugh, K P

    2016-01-01

    From birth to death the human host immune system interacts with bacterial cells. Biofilms are communities of microbes embedded in matrices composed of extracellular polymeric substance (EPS), and have been implicated in both the healthy microbiome and disease states. The immune system recognizes many different bacterial patterns, molecules, and antigens, but these components can be camouflaged in the biofilm mode of growth. Instead, immune cells come into contact with components of the EPS matrix, a diverse, hydrated mixture of extracellular DNA (bacterial and host), proteins, polysaccharides, and lipids. As bacterial cells transition from planktonic to biofilm-associated they produce small molecules, which can increase inflammation, induce cell death, and even cause necrosis. To survive, invading bacteria must overcome the epithelial barrier, host microbiome, complement, and a variety of leukocytes. If bacteria can evade these initial cell populations they have an increased chance at surviving and causing ongoing disease in the host. Planktonic cells are readily cleared, but biofilms reduce the effectiveness of both polymorphonuclear neutrophils and macrophages. In addition, in the presence of these cells, biofilm formation is actively enhanced, and components of host immune cells are assimilated into the EPS matrix. While pathogenic biofilms contribute to states of chronic inflammation, probiotic Lactobacillus biofilms cause a negligible immune response and, in states of inflammation, exhibit robust antiinflammatory properties. These probiotic biofilms colonize and protect the gut and vagina, and have been implicated in improved healing of damaged skin. Overall, biofilms stimulate a unique immune response that we are only beginning to understand. PMID:27571696

  19. Host Responses to Biofilm.

    PubMed

    Watters, C; Fleming, D; Bishop, D; Rumbaugh, K P

    2016-01-01

    From birth to death the human host immune system interacts with bacterial cells. Biofilms are communities of microbes embedded in matrices composed of extracellular polymeric substance (EPS), and have been implicated in both the healthy microbiome and disease states. The immune system recognizes many different bacterial patterns, molecules, and antigens, but these components can be camouflaged in the biofilm mode of growth. Instead, immune cells come into contact with components of the EPS matrix, a diverse, hydrated mixture of extracellular DNA (bacterial and host), proteins, polysaccharides, and lipids. As bacterial cells transition from planktonic to biofilm-associated they produce small molecules, which can increase inflammation, induce cell death, and even cause necrosis. To survive, invading bacteria must overcome the epithelial barrier, host microbiome, complement, and a variety of leukocytes. If bacteria can evade these initial cell populations they have an increased chance at surviving and causing ongoing disease in the host. Planktonic cells are readily cleared, but biofilms reduce the effectiveness of both polymorphonuclear neutrophils and macrophages. In addition, in the presence of these cells, biofilm formation is actively enhanced, and components of host immune cells are assimilated into the EPS matrix. While pathogenic biofilms contribute to states of chronic inflammation, probiotic Lactobacillus biofilms cause a negligible immune response and, in states of inflammation, exhibit robust antiinflammatory properties. These probiotic biofilms colonize and protect the gut and vagina, and have been implicated in improved healing of damaged skin. Overall, biofilms stimulate a unique immune response that we are only beginning to understand.

  20. Exchange of hosts: can agaonid fig wasps reproduce successfully in the figs of non-host Ficus?

    NASA Astrophysics Data System (ADS)

    Yang, Pei; Li, Zongbo; Peng, Yanqiong; Yang, Darong

    2012-03-01

    In the obligate mutualism between figs ( Ficus) and their specific pollinators (Chalcidoidea, Agaonidae), each species of fig wasp typically reproduces in figs of a single host species. Host specificity is maintained largely because pollinators are attracted to tree-specific volatiles released from their host figs, but whether the wasps can reproduce if they enter figs of non-host species is unclear. We investigated the reproductive success of Ceratosolen emarginatus (associated with Ficus auriculata) and Ceratosolen sp. (associated with F. hainanensis) in atypical hosts by experimentally introducing foundresses into host and non-host figs. F. auriculata figs entered by Ceratosolen sp. were more likely to abort than if entered by C. emarginatus, but abortion of F. hainanensis figs was not affected by pollinator species. Single C. emarginatus foundresses produced more but smaller offspring in F. hainanensis than in their normal host. Conversely Ceratosolen sp. produced fewer but larger offspring in F. auriculata than in their normal host, probably as a result of having longer to develop. Mean style length differences, relative to the lengths of the wasps' ovipositors, may have dictated the number of offspring produced, with oviposition made easier by the shorter styles in F. hainanensis figs. Our results imply that, in addition to morphological constraints and tree-specific volatiles, reduced reproductive success in atypical hosts can be another factor maintaining host specificity, but for other species only behavioural changes are required for host switching to occur.

  1. Differential reproductive success favours strong host preference in a highly specialized brood parasite

    PubMed Central

    De Mársico, María C; Reboreda, Juan C

    2008-01-01

    Obligate avian brood parasites show dramatic variation in the degree to which they are host specialists or host generalists. The screaming cowbird Molothrus rufoaxillaris is one of the most specialized brood parasites, using a single host, the bay-winged cowbird (Agelaioides badius) over most of its range. Coevolutionary theory predicts increasing host specificity the longer the parasite interacts with a particular avian community, as hosts evolve defences that the parasite cannot counteract. According to this view, host specificity can be maintained if screaming cowbirds avoid parasitizing potentially suitable hosts that have developed effective defences against parasitic females or eggs. Specialization may also be favoured, even in the absence of host defences, if the parasite's reproductive success in alternative hosts is lower than that in the main host. We experimentally tested these hypotheses using as alternative hosts two suitable but unparasitized species: house wrens (Troglodytes aedon) and chalk-browed mockingbirds (Mimus saturninus). We assessed host defences against parasitic females and eggs, and reproductive success of the parasite in current and alternative hosts. Alternative hosts did not discriminate against screaming cowbird females or eggs. Egg survival and hatching success were similarly high in current and alternative hosts, but the survival of parasitic chicks was significantly lower in alternative hosts. Our results indicate that screaming cowbirds have the potential to colonize novel hosts, but higher reproductive success in the current host may favour host fidelity. PMID:18647716

  2. Differential reproductive success favours strong host preference in a highly specialized brood parasite.

    PubMed

    De Mársico, María C; Reboreda, Juan C

    2008-11-01

    Obligate avian brood parasites show dramatic variation in the degree to which they are host specialists or host generalists. The screaming cowbird Molothrus rufoaxillaris is one of the most specialized brood parasites, using a single host, the bay-winged cowbird (Agelaioides badius) over most of its range. Coevolutionary theory predicts increasing host specificity the longer the parasite interacts with a particular avian community, as hosts evolve defences that the parasite cannot counteract. According to this view, host specificity can be maintained if screaming cowbirds avoid parasitizing potentially suitable hosts that have developed effective defences against parasitic females or eggs. Specialization may also be favoured, even in the absence of host defences, if the parasite's reproductive success in alternative hosts is lower than that in the main host. We experimentally tested these hypotheses using as alternative hosts two suitable but unparasitized species: house wrens (Troglodytes aedon) and chalk-browed mockingbirds (Mimus saturninus). We assessed host defences against parasitic females and eggs, and reproductive success of the parasite in current and alternative hosts. Alternative hosts did not discriminate against screaming cowbird females or eggs. Egg survival and hatching success were similarly high in current and alternative hosts, but the survival of parasitic chicks was significantly lower in alternative hosts. Our results indicate that screaming cowbirds have the potential to colonize novel hosts, but higher reproductive success in the current host may favour host fidelity.

  3. Host Phylogeny Determines Viral Persistence and Replication in Novel Hosts

    PubMed Central

    Longdon, Ben; Hadfield, Jarrod D.; Webster, Claire L.

    2011-01-01

    Pathogens switching to new hosts can result in the emergence of new infectious diseases, and determining which species are likely to be sources of such host shifts is essential to understanding disease threats to both humans and wildlife. However, the factors that determine whether a pathogen can infect a novel host are poorly understood. We have examined the ability of three host-specific RNA-viruses (Drosophila sigma viruses from the family Rhabdoviridae) to persist and replicate in 51 different species of Drosophilidae. Using a novel analytical approach we found that the host phylogeny could explain most of the variation in viral replication and persistence between different host species. This effect is partly driven by viruses reaching a higher titre in those novel hosts most closely related to the original host. However, there is also a strong effect of host phylogeny that is independent of the distance from the original host, with viral titres being similar in groups of related hosts. Most of this effect could be explained by variation in general susceptibility to all three sigma viruses, as there is a strong phylogenetic correlation in the titres of the three viruses. These results suggest that the source of new emerging diseases may often be predictable from the host phylogeny, but that the effect may be more complex than simply causing most host shifts to occur between closely related hosts. PMID:21966271

  4. Competitive environments induce shifts in host fidelity.

    PubMed

    Rova, E; Björklund, M

    2010-08-01

    Recent models support the idea of sympatric speciation as a result of the joint effects of disruptive selection and assortative mating. We present experimental data, testing models of speciation through frequency-dependent selection. We show that under high competition on a mixture of resources/hosts, strains of the Seed beetle, Callosobruchus maculatus, change their host fidelity and evolve a more generalistic behaviour in resource utilization among females. The change in host fidelity did not result in disruptive selection and was not followed by assortative mating. This means that only one of three fundamental prerequisites for sympatric speciation evolved as a result of the frequency-dependent selection. We conclude that for this process to work, a shift to a novel food resource as a result of selection must also lead to a loss of preference for the original resource such that individuals are only able to use either one of the two.

  5. Comparing mechanisms of host manipulation across host and parasite taxa.

    PubMed

    Lafferty, Kevin D; Shaw, Jenny C

    2013-01-01

    Parasites affect host behavior in several ways. They can alter activity, microhabitats or both. For trophically transmitted parasites (the focus of our study), decreased activity might impair the ability of hosts to respond to final-host predators, and increased activity and altered microhabitat choice might increase contact rates between hosts and final-host predators. In an analysis of trophically transmitted parasites, more parasite groups altered activity than altered microhabitat choice. Parasites that infected vertebrates were more likely to impair the host's reaction to predators, whereas parasites that infected invertebrates were more likely to increase the host's contact with predators. The site of infection might affect how parasites manipulate their hosts. For instance, parasites in the central nervous system seem particularly suited to manipulating host behavior. Manipulative parasites commonly occupy the body cavity, muscles and central nervous systems of their hosts. Acanthocephalans in the data set differed from other taxa in that they occurred exclusively in the body cavity of invertebrates. In addition, they were more likely to alter microhabitat choice than activity. Parasites in the body cavity (across parasite types) were more likely to be associated with increased host contact with predators. Parasites can manipulate the host through energetic drain, but most parasites use more sophisticated means. For instance, parasites target four physiological systems that shape behavior in both invertebrates and vertebrates: neural, endocrine, neuromodulatory and immunomodulatory. The interconnections between these systems make it difficult to isolate specific mechanisms of host behavioral manipulation.

  6. Evolution of Host Defense against Multiple Enemy Populations.

    PubMed

    Toor, Jaspreet; Best, Alex

    2016-03-01

    Natural and managed populations are embedded within complex ecological communities, where they face multiple enemies. Experimental studies have shown that the evolution of host defense mechanisms to a focal enemy is impacted by the surrounding enemy community. Theoretically, the evolution of host defenses against a single enemy population, typically parasites, has been widely studied, but only recently has the impact of community interactions on host-parasite evolution been looked at. In this article, we theoretically examine the evolutionary behavior of a host population that must allocate defenses between two enemy populations, parasites and predators, with defense against one enemy constraining defense against the other. We show that in simpler models the composition of the enemy community plays the key role in determining the defense strategy of the hosts, with the hosts building up defenses against the enemy population posing a larger threat. However, this simple driver is shown to break down when there is significant recovery and reproduction from infected hosts. Additionally, we find that most host diversity is likely to occur when there is a combined high risk of infection and predation, in common with experimental studies. Our results therefore provide vital insight into the ecological feedbacks that drive the evolution of host defense against multiple enemy populations.

  7. Gravisensitivity of various host plant -virus systems in simulated microgravity

    NASA Astrophysics Data System (ADS)

    Mishchenko, Lidiya; Taran, Oksana; Gordejchyk, Olga

    plants had lower X-virus antigen content, compared with negative control. In plants, cultivated without clinostating, PVX antigen content was 5-10 times greater than on negative control variants. Prolonged (over 43 days) clinostating, depending on potato plant genotype, may cause both simulation and impeding of the accumulation of Y-virus antigens in potato plants. Studying the interaction between the host plant and PVM, we found that prolonged clinorotation at first reduced the antigen content by 25-30% compared with stationary control. Further on after 44 days of experimentation, the antigen content increased with more intensive increase in non-clinostated plants. Thus, prolonged clinostating reduced the intensity of anti-gen accumulation but did not stop it completely. We admit that proves a low sensitivity of the system PVM -potato plant to simulated microgravity. The phenomena of PVX reproduction in simulated microgravity may find on employment in present-day biotechnologies.

  8. Hosting a Katrina Evacuee.

    NASA Astrophysics Data System (ADS)

    Hoagland, David

    2008-03-01

    No individual or institution anticipated the impact on the academic research community of hurricane Katrina. When Tulane physicist Wayne Reed asked me to host his research group just a day or two after the disaster, with no authorization or understanding of the commitment, I agreed immediately and then pondered implications. Fortunately, colleagues helped in making the commitment real, only the bureaucracy of my public university posing small hindrances. Industry was remarkably generous in providing Reed with significant ``loaner'' equipment, and amazingly, a suite of custom Reed experiments was running within weeks. At the end, the most productive collaborations for Reed seemed not to have been with my group, with its similar research, but to other groups at my institution, particularly the synthetic chemists, who gained access to methods previously unique to Tulane while offering samples previously unique to UMass. Quickly designed projects exploiting this match turned out remarkably productive. Although begun with trepidation, hosting of Reed had huge positive benefits to me and UMass, and I believe, also to Reed and Tulane. Some key lessons for the future: (i) industry has capacity and willingness to help academic research during disruption (ii) commitment of a host institution must be immediate, without a wait for formal approvals or arrangement of special funding -- delay leads only to discouragement, (iii) continuing academic progress of displaced students must come first, and (iv) intellectual synergy rather than overlap should be the basis for seeking a host. Lastly, NSF or other funding agency should consider a program directly addressing the research needs of unexpectedly disrupted academic scientists, and most particularly, graduate students who face greatly extended studies.

  9. Nocardia species: host-parasite relationships.

    PubMed Central

    Beaman, B L; Beaman, L

    1994-01-01

    The nocardiae are bacteria belonging to the aerobic actinomycetes. They are an important part of the normal soil microflora worldwide. The type species, Nocardia asteroides, and N. brasiliensis, N. farcinica, N. otitidiscaviarum, N. nova, and N. transvalensis cause a variety of diseases in both normal and immunocompromised humans and animals. The mechanisms of pathogenesis are complex, not fully understood, and include the capacity to evade or neutralize the myriad microbicidal activities of the host. The relative virulence of N. asteroides correlates with the ability to inhibit phagosome-lysosome fusion in phagocytes; to neutralize phagosomal acidification; to detoxify the microbicidal products of oxidative metabolism; to modify phagocyte function; to grow within phagocytic cells; and to attach to, penetrate, and grow within host cells. Both activated macrophages and immunologically specific T lymphocytes constitute the major mechanisms for host resistance to nocardial infection, whereas B lymphocytes and humoral immunity do not appear to be as important in protecting the host. Thus, the nocardiae are facultative intracellular pathogens that can persist within the host, probably in a cryptic form (L-form), for life. Silent invasion of brain cells by some Nocardia strains can induce neurodegeneration in experimental animals; however, the role of nocardiae in neurodegenerative diseases in humans needs to be investigated. Images PMID:8055469

  10. Fatty acid-producing hosts

    DOEpatents

    Pfleger, Brian F; Lennen, Rebecca M

    2013-12-31

    Described are hosts for overproducing a fatty acid product such as a fatty acid. The hosts include an exogenous nucleic acid encoding a thioesterase and, optionally, an exogenous nucleic acid encoding an acetyl-CoA carboxylase, wherein an acyl-CoA synthetase in the hosts are functionally delected. The hosts prefereably include the nucleic acid encoding the thioesterase at an intermediate copy number. The hosts are preferably recominantly stable and growth-competent at 37.degree. C. Methods of producing a fatty acid product comprising culturing such hosts at 37.degree. C. are also described.

  11. Graft-versus-host disease

    MedlinePlus

    GVHD; Bone marrow transplant - graft-versus-host disease; Stem cell transplant - graft-versus-host disease; Allogeneic transplant - ... GVHD may occur after a bone marrow, or stem cell, transplant in which someone receives bone marrow ...

  12. HostPhinder: A Phage Host Prediction Tool.

    PubMed

    Villarroel, Julia; Kleinheinz, Kortine Annina; Jurtz, Vanessa Isabell; Zschach, Henrike; Lund, Ole; Nielsen, Morten; Larsen, Mette Voldby

    2016-05-04

    The current dramatic increase of antibiotic resistant bacteria has revitalised the interest in bacteriophages as alternative antibacterial treatment. Meanwhile, the development of bioinformatics methods for analysing genomic data places high-throughput approaches for phage characterization within reach. Here, we present HostPhinder, a tool aimed at predicting the bacterial host of phages by examining the phage genome sequence. Using a reference database of 2196 phages with known hosts, HostPhinder predicts the host species of a query phage as the host of the most genomically similar reference phages. As a measure of genomic similarity the number of co-occurring k-mers (DNA sequences of length k) is used. Using an independent evaluation set, HostPhinder was able to correctly predict host genus and species for 81% and 74% of the phages respectively, giving predictions for more phages than BLAST and significantly outperforming BLAST on phages for which both had predictions. HostPhinder predictions on phage draft genomes from the INTESTI phage cocktail corresponded well with the advertised targets of the cocktail. Our study indicates that for most phages genomic similarity correlates well with related bacterial hosts. HostPhinder is available as an interactive web service [1] and as a stand alone download from the Docker registry [2].

  13. HostPhinder: A Phage Host Prediction Tool.

    PubMed

    Villarroel, Julia; Kleinheinz, Kortine Annina; Jurtz, Vanessa Isabell; Zschach, Henrike; Lund, Ole; Nielsen, Morten; Larsen, Mette Voldby

    2016-01-01

    The current dramatic increase of antibiotic resistant bacteria has revitalised the interest in bacteriophages as alternative antibacterial treatment. Meanwhile, the development of bioinformatics methods for analysing genomic data places high-throughput approaches for phage characterization within reach. Here, we present HostPhinder, a tool aimed at predicting the bacterial host of phages by examining the phage genome sequence. Using a reference database of 2196 phages with known hosts, HostPhinder predicts the host species of a query phage as the host of the most genomically similar reference phages. As a measure of genomic similarity the number of co-occurring k-mers (DNA sequences of length k) is used. Using an independent evaluation set, HostPhinder was able to correctly predict host genus and species for 81% and 74% of the phages respectively, giving predictions for more phages than BLAST and significantly outperforming BLAST on phages for which both had predictions. HostPhinder predictions on phage draft genomes from the INTESTI phage cocktail corresponded well with the advertised targets of the cocktail. Our study indicates that for most phages genomic similarity correlates well with related bacterial hosts. HostPhinder is available as an interactive web service [1] and as a stand alone download from the Docker registry [2]. PMID:27153081

  14. Comparing mechanisms of host manipulation across host and parasite taxa

    USGS Publications Warehouse

    Lafferty, Kevin D.; Shaw, Jenny C.

    2013-01-01

    Parasites affect host behavior in several ways. They can alter activity, microhabitats or both. For trophically transmitted parasites (the focus of our study), decreased activity might impair the ability of hosts to respond to final-host predators, and increased activity and altered microhabitat choice might increase contact rates between hosts and final-host predators. In an analysis of trophically transmitted parasites, more parasite groups altered activity than altered microhabitat choice. Parasites that infected vertebrates were more likely to impair the host’s reaction to predators, whereas parasites that infected invertebrates were more likely to increase the host’s contact with predators. The site of infection might affect how parasites manipulate their hosts. For instance, parasites in the central nervous system seem particularly suited to manipulating host behavior. Manipulative parasites commonly occupy the body cavity, muscles and central nervous systems of their hosts. Acanthocephalans in the data set differed from other taxa in that they occurred exclusively in the body cavity of invertebrates. In addition, they were more likely to alter microhabitat choice than activity. Parasites in the body cavity (across parasite types) were more likely to be associated with increased host contact with predators. Parasites can manipulate the host through energetic drain, but most parasites use more sophisticated means. For instance, parasites target four physiological systems that shape behavior in both invertebrates and vertebrates: neural, endocrine, neuromodulatory and immunomodulatory. The interconnections between these systems make it difficult to isolate specific mechanisms of host behavioral manipulation.

  15. HostPhinder: A Phage Host Prediction Tool

    PubMed Central

    Villarroel, Julia; Kleinheinz, Kortine Annina; Jurtz, Vanessa Isabell; Zschach, Henrike; Lund, Ole; Nielsen, Morten; Larsen, Mette Voldby

    2016-01-01

    The current dramatic increase of antibiotic resistant bacteria has revitalised the interest in bacteriophages as alternative antibacterial treatment. Meanwhile, the development of bioinformatics methods for analysing genomic data places high-throughput approaches for phage characterization within reach. Here, we present HostPhinder, a tool aimed at predicting the bacterial host of phages by examining the phage genome sequence. Using a reference database of 2196 phages with known hosts, HostPhinder predicts the host species of a query phage as the host of the most genomically similar reference phages. As a measure of genomic similarity the number of co-occurring k-mers (DNA sequences of length k) is used. Using an independent evaluation set, HostPhinder was able to correctly predict host genus and species for 81% and 74% of the phages respectively, giving predictions for more phages than BLAST and significantly outperforming BLAST on phages for which both had predictions. HostPhinder predictions on phage draft genomes from the INTESTI phage cocktail corresponded well with the advertised targets of the cocktail. Our study indicates that for most phages genomic similarity correlates well with related bacterial hosts. HostPhinder is available as an interactive web service [1] and as a stand alone download from the Docker registry [2]. PMID:27153081

  16. Allergic Host Defenses

    PubMed Central

    Palm, Noah W.; Rosenstein, Rachel K.

    2012-01-01

    Allergies are generally thought to be a detrimental outcome of a mistargeted immune response that evolved to provide immunity to macro-parasites. Here we present arguments to suggest that allergic immunity plays an important role in host defense against noxious environmental substances, including venoms, hematophagous fluids, environmental xenobiotics and irritants. We argue that appropriately targeted allergic reactions are beneficial, although they can become detrimental when excessive. Furthermore, we suggest that allergic hypersensitivity evolved to elicit anticipatory responses and to promote avoidance of suboptimal environments. PMID:22538607

  17. Host Specificity of Bacterial Pathogens

    PubMed Central

    Bäumler, Andreas; Fang, Ferric C.

    2013-01-01

    Most pathogens are able to infect multiple hosts but some are highly adapted to a single-host species. A detailed understanding of the basis of host specificity can provide important insights into molecular pathogenesis, the evolution of pathogenic microbes, and the potential for pathogens to cross the species barrier to infect new hosts. Comparative genomics and the development of humanized mouse models have provided important new tools with which to explore the basis of generalism and specialism. This review will examine host specificity of bacterial pathogens with a focus on generalist and specialist serovars of Salmonella enterica. PMID:24296346

  18. Multidimensionality in host manipulation mimicked by serotonin injection.

    PubMed

    Perrot-Minnot, Marie-Jeanne; Sanchez-Thirion, Kevin; Cézilly, Frank

    2014-12-01

    Manipulative parasites often alter the phenotype of their hosts along multiple dimensions. 'Multidimensionality' in host manipulation could consist in the simultaneous alteration of several physiological pathways independently of one another, or proceed from the disruption of some key physiological parameter, followed by a cascade of effects. We compared multidimensionality in 'host manipulation' between two closely related amphipods, Gammarus fossarum and Gammarus pulex, naturally and experimentally infected with Pomphorhynchus laevis (Acanthocephala), respectively. To that end, we calculated in each host-parasite association the effect size of the difference between infected and uninfected individuals for six different traits (activity, phototaxis, geotaxis, attraction to conspecifics, refuge use and metabolic rate). The effects sizes were highly correlated between host-parasite associations, providing evidence for a relatively constant 'infection syndrome'. Using the same methodology, we compared the extent of phenotypic alterations induced by an experimental injection of serotonin (5-HT) in uninfected G. pulex to that induced by experimental or natural infection with P. laevis. We observed a significant correlation between effect sizes across the six traits, indicating that injection with 5-HT can faithfully mimic the 'infection syndrome'. This is, to our knowledge, the first experimental evidence that multidimensionality in host manipulation can proceed, at least partly, from the disruption of some major physiological mechanism.

  19. Multidimensionality in host manipulation mimicked by serotonin injection.

    PubMed

    Perrot-Minnot, Marie-Jeanne; Sanchez-Thirion, Kevin; Cézilly, Frank

    2014-12-01

    Manipulative parasites often alter the phenotype of their hosts along multiple dimensions. 'Multidimensionality' in host manipulation could consist in the simultaneous alteration of several physiological pathways independently of one another, or proceed from the disruption of some key physiological parameter, followed by a cascade of effects. We compared multidimensionality in 'host manipulation' between two closely related amphipods, Gammarus fossarum and Gammarus pulex, naturally and experimentally infected with Pomphorhynchus laevis (Acanthocephala), respectively. To that end, we calculated in each host-parasite association the effect size of the difference between infected and uninfected individuals for six different traits (activity, phototaxis, geotaxis, attraction to conspecifics, refuge use and metabolic rate). The effects sizes were highly correlated between host-parasite associations, providing evidence for a relatively constant 'infection syndrome'. Using the same methodology, we compared the extent of phenotypic alterations induced by an experimental injection of serotonin (5-HT) in uninfected G. pulex to that induced by experimental or natural infection with P. laevis. We observed a significant correlation between effect sizes across the six traits, indicating that injection with 5-HT can faithfully mimic the 'infection syndrome'. This is, to our knowledge, the first experimental evidence that multidimensionality in host manipulation can proceed, at least partly, from the disruption of some major physiological mechanism. PMID:25339729

  20. The Drosophila melanogaster host model

    PubMed Central

    Igboin, Christina O.; Griffen, Ann L.; Leys, Eugene J.

    2012-01-01

    The deleterious and sometimes fatal outcomes of bacterial infectious diseases are the net result of the interactions between the pathogen and the host, and the genetically tractable fruit fly, Drosophila melanogaster, has emerged as a valuable tool for modeling the pathogen–host interactions of a wide variety of bacteria. These studies have revealed that there is a remarkable conservation of bacterial pathogenesis and host defence mechanisms between higher host organisms and Drosophila. This review presents an in-depth discussion of the Drosophila immune response, the Drosophila killing model, and the use of the model to examine bacterial–host interactions. The recent introduction of the Drosophila model into the oral microbiology field is discussed, specifically the use of the model to examine Porphyromonas gingivalis–host interactions, and finally the potential uses of this powerful model system to further elucidate oral bacterial-host interactions are addressed. PMID:22368770

  1. The impact of parasitoid emergence time on host-parasitoid population dynamics.

    PubMed

    Cobbold, Christina A; Roland, Jens; Lewis, Mark A

    2009-01-01

    We investigate the effect of parasitoid phenology on host-parasitoid population cycles. Recent experimental research has shown that parasitized hosts can continue to interact with their unparasitized counterparts through competition. Parasitoid phenology, in particular the timing of emergence from the host, determines the duration of this competition. We construct a discrete-time host-parasitoid model in which within-generation dynamics associated with parasitoid timing is explicitly incorporated. We found that late-emerging parasitoids induce less severe, but more frequent, host outbreaks, independent of the choice of competition model. The competition experienced by the parasitized host reduces the parasitoids' numerical response to changes in host numbers, preventing the 'boom-bust' dynamics associated with more efficient parasitoids. We tested our findings against experimental data for the forest tent caterpillar (Malacosoma disstria Hübner) system, where a large number of consecutive years at a high host density is synonymous with severe forest damage.

  2. Poxvirus host cell entry.

    PubMed

    Schmidt, Florian Ingo; Bleck, Christopher Karl Ernst; Mercer, Jason

    2012-02-01

    Poxviruses are characterized by their large size, complex composition, and cytoplasmic life cycle. They produce two types of infectious particles: mature virions (MVs) and extracellular virions (EVs). Both MVs and EVs of vaccinia virus, the model poxvirus, take advantage of host cell endocytosis for internalization: they activate macropinocytosis-the most suitable form of endocytosis for large particles. Although largely dependent on the same cellular machinery, MV and EV entry differs with regard to the mechanisms used to trigger macropinocytosis and to undergo fusion. While EVs have to shed an additional membrane to expose the fusion complex, MV fusion requires the inactivation of fusion inhibitory proteins absent in EVs. This review highlights recent advances in the understanding of poxvirus MV and EV cell entry. PMID:22440962

  3. Host responses to the pathogen Mycobacterium avium subsp. paratuberculosis and beneficial microbes exhibit host sex specificity.

    PubMed

    Karunasena, Enusha; McMahon, K Wyatt; Chang, David; Brashears, Mindy M

    2014-08-01

    Differences between microbial pathogenesis in male and female hosts are well characterized in disease conditions connected to sexual transmission. However, limited biological insight is available on variances attributed to sex specificity in host-microbe interactions, and it is most often a minimized variable outside these transmission events. In this work, we studied two gut microbes-a pathogen, Mycobacterium avium subsp. paratuberculosis, and a probiotic, Lactobacillus animalis NP-51-and the interaction between each agent and the male and female gastrointestinal systems. This trial was conducted in BALB/c mice (n=5 per experimental group and per sex at a given time point), with analysis at four time points over 180 days. Host responses to M.avium subsp. paratuberculosis and L. animalis were sensitive to sex. Cytokines that were significantly different (P ≤ 0.05) betweenthe sexes included interleukin-1α/β (IL-1α/β), IL-17, IL-6, IL-10, IL-12, and gamma interferon (IFN-) and were dependent on experimental conditions. However, granulocyte-macrophage colony-stimulating factor (GM-CSF), vascular endothelial growth factor (VEGF), and IL-13/23 showed no sex specificity. A metabolomics study indicated a 0.5- to 2.0-fold (log2 scale) increase in short-chain fatty acids (butyrate and acetate) in males and greater increases in o-phosphocholine or histidine from female colon tissues; variances distinct to each sex were observed with age or long-term probiotic consumption. Two genera, Staphylococcus and Roseburia, were consistently overrepresented in females compared to males; other species were specific to one sex but fluctuated depending on experimental conditions. The differences observed suggest that male and female gut tissues and microbiota respond to newly introduced microorganisms differently and that gut-associated microorganisms with host immune system responses and metabolic activity are supported by biology distinct to the host sex.

  4. Host Responses to the Pathogen Mycobacterium avium subsp. paratuberculosis and Beneficial Microbes Exhibit Host Sex Specificity

    PubMed Central

    McMahon, K. Wyatt; Chang, David; Brashears, Mindy M.

    2014-01-01

    Differences between microbial pathogenesis in male and female hosts are well characterized in disease conditions connected to sexual transmission. However, limited biological insight is available on variances attributed to sex specificity in host-microbe interactions, and it is most often a minimized variable outside these transmission events. In this work, we studied two gut microbes—a pathogen, Mycobacterium avium subsp. paratuberculosis, and a probiotic, Lactobacillus animalis NP-51—and the interaction between each agent and the male and female gastrointestinal systems. This trial was conducted in BALB/c mice (n = 5 per experimental group and per sex at a given time point), with analysis at four time points over 180 days. Host responses to M. avium subsp. paratuberculosis and L. animalis were sensitive to sex. Cytokines that were significantly different (P ≤ 0.05) between the sexes included interleukin-1α/β (IL-1α/β), IL-17, IL-6, IL-10, IL-12, and gamma interferon (IFN-γ) and were dependent on experimental conditions. However, granulocyte-macrophage colony-stimulating factor (GM-CSF), vascular endothelial growth factor (VEGF), and IL-13/23 showed no sex specificity. A metabolomics study indicated a 0.5- to 2.0-fold (log2 scale) increase in short-chain fatty acids (butyrate and acetate) in males and greater increases in o-phosphocholine or histidine from female colon tissues; variances distinct to each sex were observed with age or long-term probiotic consumption. Two genera, Staphylococcus and Roseburia, were consistently overrepresented in females compared to males; other species were specific to one sex but fluctuated depending on experimental conditions. The differences observed suggest that male and female gut tissues and microbiota respond to newly introduced microorganisms differently and that gut-associated microorganisms with host immune system responses and metabolic activity are supported by biology distinct to the host sex. PMID:24814797

  5. A tuberculosis ontology for host systems biology.

    PubMed

    Levine, David M; Dutta, Noton K; Eckels, Josh; Scanga, Charles; Stein, Catherine; Mehra, Smriti; Kaushal, Deepak; Karakousis, Petros C; Salamon, Hugh

    2015-09-01

    A major hurdle facing tuberculosis (TB) investigators who want to utilize a rapidly growing body of data from both systems biology approaches and omics technologies is the lack of a standard vocabulary for data annotation and reporting. Lacking a means to readily compare samples from different research groups, a significant quantity of potentially informative data is largely ignored by researchers. To facilitate standardizing data across studies, a simple ontology of TB terms was developed to provide a common vocabulary for annotating data sets. New terminology was developed to address animal models and experimental systems, and existing clinically focused terminology was modified and adapted. This ontology can be used to annotate host TB data in public databases and collaborations, thereby standardizing database searches and allowing researchers to more easily compare results. To demonstrate the utility of a standard TB ontology for host systems biology, a web application was developed to annotate and compare human and animal model gene expression data sets.

  6. A Tuberculosis Ontology for Host Systems Biology

    PubMed Central

    Levine, David M.; Dutta, Noton K.; Eckels, Josh; Scanga, Charles; Stein, Catherine; Mehra, Smriti; Kaushal, Deepak; Karakousis, Petros C.; Salamon, Hugh

    2015-01-01

    Summary A major hurdle facing tuberculosis (TB) investigators who want to utilize a rapidly growing body of data from both systems biology approaches and omics technologies is the lack of a standard vocabulary for data annotation and reporting. Lacking a means to readily compare samples from different research groups, a significant quantity of potentially informative data is largely ignored by researchers. To facilitate standardizing data across studies, a simple ontology of TB terms was developed to provide a common vocabulary for annotating data sets. New terminology was developed to address animal models and experimental systems, and existing clinically focused terminology was modified and adapted. This ontology can be used to annotate host TB data in public databases and collaborations, thereby standardizing database searches and allowing researchers to more easily compare results. To demonstrate the utility of a standard TB ontology for host systems biology, a web application was developed to annotate and compare human and animal model gene expression data sets. PMID:26190839

  7. Echinococcus multilocularis and Its Intermediate Host: A Model of Parasite-Host Interplay

    PubMed Central

    Vuitton, Dominique Angèle; Gottstein, Bruno

    2010-01-01

    Host-parasite interactions in the E. multilocularis-intermediate host model depend on a subtle balance between cellular immunity, which is responsible for host's resistance towards the metacestode, the larval stage of the parasite, and tolerance induction and maintenance. The pathological features of alveolar echinococcosis. the disease caused by E. multilocularis, are related both to parasitic growth and to host's immune response, leading to fibrosis and necrosis, The disease spectrum is clearly dependent on the genetic background of the host as well as on acquired disturbances of Th1-related immunity. The laminated layer of the metacestode, and especially its carbohydrate components, plays a major role in tolerance induction. Th2-type and anti-inflammatory cytokines, IL-10 and TGF-β, as well as nitric oxide, are involved in the maintenance of tolerance and partial inhibition of cytotoxic mechanisms. Results of studies in the experimental mouse model and in patients suggest that immune modulation with cytokines, such as interferon-α, or with specific antigens could be used in the future to treat patients with alveolar echinococcosis and/or to prevent this very severe parasitic disease. PMID:20339517

  8. Ethanol production by recombinant hosts

    DOEpatents

    Ingram, Lonnie O.; Beall, David S.; Burchhardt, Gerhard F. H.; Guimaraes, Walter V.; Ohta, Kazuyoshi; Wood, Brent E.; Shanmugam, Keelnatham T.

    1995-01-01

    Novel plasmids comprising genes which code for the alcohol dehydrogenase and pyruvate decarboxylase are described. Also described are recombinant hosts which have been transformed with genes coding for alcohol dehydrogenase and pyruvate. By virtue of their transformation with these genes, the recombinant hosts are capable of producing significant amounts of ethanol as a fermentation product. Also disclosed are methods for increasing the growth of recombinant hosts and methods for reducing the accumulation of undesirable metabolic products in the growth medium of these hosts. Also disclosed are recombinant host capable of producing significant amounts of ethanol as a fermentation product of oligosaccharides and plasmids comprising genes encoding polysaccharases, in addition to the genes described above which code for the alcohol dehydrogenase and pyruvate decarboxylase. Further, methods are described for producing ethanol from oligomeric feedstock using the recombinant hosts described above. Also provided is a method for enhancing the production of functional proteins in a recombinant host comprising overexpressing an adhB gene in the host. Further provided are process designs for fermenting oligosaccharide-containing biomass to ethanol.

  9. Ethanol production by recombinant hosts

    DOEpatents

    Fowler, David E.; Horton, Philip G.; Ben-Bassat, Arie

    1996-01-01

    Novel plasmids comprising genes which code for the alcohol dehydrogenase and pyruvate decarboxylase are described. Also described are recombinant hosts which have been transformed with genes coding for alcohol dehydrogenase and pyruvate. By virtue of their transformation with these genes, the recombinant hosts are capable of producing significant amounts of ethanol as a fermentation product. Also disclosed are methods for increasing the growth of recombinant hosts and methods for reducing the accumulation of undesirable metabolic products in the growth medium of these hosts. Also disclosed are recombinant host capable of producing significant amounts of ethanol as a fermentation product of oligosaccharides and plasmids comprising genes encoding polysaccharases, in addition to the genes described above which code for the alcohol dehydrogenase and pyruvate decarboxylase. Further, methods are described for producing ethanol from oligomeric feedstock using the recombinant hosts described above. Also provided is a method for enhancing the production of functional proteins in a recombinant host comprising overexpressing an adhB gene in the host. Further provided are process designs for fermenting oligosaccharide-containing biomass to ethanol.

  10. Castrating parasites and colonial hosts.

    PubMed

    Hartikainen, H; Okamura, B

    2012-04-01

    Trajectories of life-history traits such as growth and reproduction generally level off with age and increasing size. However, colonial animals may exhibit indefinite, exponential growth via modular iteration thus providing a long-lived host source for parasite exploitation. In addition, modular iteration entails a lack of germ line sequestration. Castration of such hosts by parasites may therefore be impermanent or precluded, unlike the general case for unitary animal hosts. Despite these intriguing correlates of coloniality, patterns of colonial host exploitation have not been well studied. We examined these patterns by characterizing the responses of a myxozoan endoparasite, Tetracapsuloides bryosalmonae, and its colonial bryozoan host, Fredericella sultana, to 3 different resource levels. We show that (1) the development of infectious stages nearly always castrates colonies regardless of host condition, (2) castration reduces partial mortality and (3) development of transmission stages is resource-mediated. Unlike familiar castrator-host systems, this system appears to be characterized by periodic rather than permanent castration. Periodic castration may be permitted by 2 key life history traits: developmental cycling of the parasite between quiescent (covert infections) and virulent infectious stages (overt infections) and the absence of germ line sequestration which allows host reproduction in between bouts of castration.

  11. Stennis hosts 2010 Special Olympics

    NASA Technical Reports Server (NTRS)

    2010-01-01

    Sarah Johnson, 28, of Gulfport, carries in the Olympic torch to signal the start of the 2010 Area III Special Olympic games at NASA's John C. Stennis Space Center on March 27. Stennis volunteers hosted special needs athletes from across the area for the event. Stennis is an annual host of the games.

  12. Host defenses trigger salmonella's arsenal.

    PubMed

    Keestra, A Marijke; Bäumler, Andreas J

    2011-03-17

    Salmonella survives in macrophages by using a molecular syringe to deliver proteins into the host-cell cytosol where they manipulate phagocyte physiology. Arpaia and colleagues (Arpaia et al., 2011) show that deployment of this virulence factor is triggered by the very responses that are intended to confer host resistance. PMID:21402352

  13. When parasites disagree: evidence for parasite-induced sabotage of host manipulation.

    PubMed

    Hafer, Nina; Milinski, Manfred

    2015-03-01

    Host manipulation is a common parasite strategy to alter host behavior in a manner to enhance parasite fitness usually by increasing the parasite's transmission to the next host. In nature, hosts often harbor multiple parasites with agreeing or conflicting interests over host manipulation. Natural selection might drive such parasites to cooperation, compromise, or sabotage. Sabotage would occur if one parasite suppresses the manipulation of another. Experimental studies on the effect of multi-parasite interactions on host manipulation are scarce, clear experimental evidence for sabotage is elusive. We tested the effect of multiple infections on host manipulation using laboratory-bred copepods experimentally infected with the trophically transmitted tapeworm Schistocephalus solidus. This parasite is known to manipulate its host depending on its own developmental stage. Coinfecting parasites with the same aim enhance each other's manipulation but only after reaching infectivity. If the coinfecting parasites disagree over host manipulation, the infective parasite wins this conflict: the noninfective one has no effect. The winning (i.e., infective) parasite suppresses the manipulation of its noninfective competitor. This presents conclusive experimental evidence for both cooperation in and sabotage of host manipulation and hence a proof of principal that one parasite can alter and even neutralize manipulation by another.

  14. When parasites disagree: Evidence for parasite-induced sabotage of host manipulation

    PubMed Central

    Hafer, Nina; Milinski, Manfred

    2015-01-01

    Host manipulation is a common parasite strategy to alter host behavior in a manner to enhance parasite fitness usually by increasing the parasite's transmission to the next host. In nature, hosts often harbor multiple parasites with agreeing or conflicting interests over host manipulation. Natural selection might drive such parasites to cooperation, compromise, or sabotage. Sabotage would occur if one parasite suppresses the manipulation of another. Experimental studies on the effect of multi-parasite interactions on host manipulation are scarce, clear experimental evidence for sabotage is elusive. We tested the effect of multiple infections on host manipulation using laboratory-bred copepods experimentally infected with the trophically transmitted tapeworm Schistocephalus solidus. This parasite is known to manipulate its host depending on its own developmental stage. Coinfecting parasites with the same aim enhance each other's manipulation but only after reaching infectivity. If the coinfecting parasites disagree over host manipulation, the infective parasite wins this conflict: the noninfective one has no effect. The winning (i.e., infective) parasite suppresses the manipulation of its noninfective competitor. This presents conclusive experimental evidence for both cooperation in and sabotage of host manipulation and hence a proof of principal that one parasite can alter and even neutralize manipulation by another. PMID:25643621

  15. Anuran Host Species Influences Site Fidelity of Halipegus occidualis.

    PubMed

    Stigge, Heather A; Bolek, Matthew G

    2016-02-01

    Helminths often demonstrate preferential site selection in which a parasite will only occur in 1 microhabitat or a restricted portion of its fundamental niche within its host. However, factors responsible for helminth site specificity are poorly understood, and very little is known about how these factors vary among multiple host species. Some helminths, such as Halipegus occidualis, have been reported from different habitats (stomach or under the tongue) within multiple anuran host species, suggesting that the site selected varies within anuran species. This study examined the site selection by H. occidualis in 7 definitive anuran host species using experimental infections. Then, the site fidelity of H. occidualis was further tested by transplanting worms from under the tongue to the stomach and vice versa in different anuran host combinations, and the movement of worms was recorded. Halipegus occidualis individuals occupied the habitat under the tongue in 6 of 7 anuran species. However, worms always occupied the stomach of American bullfrogs (Lithobates catesbeianus) and were never found under the tongue or in the mouth of these hosts. More importantly, all worms remained in the original habitat when transplanted from the stomach to the stomach or the buccal cavity to the buccal cavity within another individual of the same amphibian species. However, when worms were transplanted from the stomach to the buccal cavity or vice versa in the same host species, the worms always migrated back to the original habitat. The main contribution of this study is that it experimentally documented the variability in the site fidelity of H. occidualis within multiple definitive host species and determined that site fidelity is not as strongly conserved in this genus as suggested previously. Additionally, this work suggests that the variation in site selection in different host species could lead to speciation of the parasites. PMID:26412569

  16. Anuran Host Species Influences Site Fidelity of Halipegus occidualis.

    PubMed

    Stigge, Heather A; Bolek, Matthew G

    2016-02-01

    Helminths often demonstrate preferential site selection in which a parasite will only occur in 1 microhabitat or a restricted portion of its fundamental niche within its host. However, factors responsible for helminth site specificity are poorly understood, and very little is known about how these factors vary among multiple host species. Some helminths, such as Halipegus occidualis, have been reported from different habitats (stomach or under the tongue) within multiple anuran host species, suggesting that the site selected varies within anuran species. This study examined the site selection by H. occidualis in 7 definitive anuran host species using experimental infections. Then, the site fidelity of H. occidualis was further tested by transplanting worms from under the tongue to the stomach and vice versa in different anuran host combinations, and the movement of worms was recorded. Halipegus occidualis individuals occupied the habitat under the tongue in 6 of 7 anuran species. However, worms always occupied the stomach of American bullfrogs (Lithobates catesbeianus) and were never found under the tongue or in the mouth of these hosts. More importantly, all worms remained in the original habitat when transplanted from the stomach to the stomach or the buccal cavity to the buccal cavity within another individual of the same amphibian species. However, when worms were transplanted from the stomach to the buccal cavity or vice versa in the same host species, the worms always migrated back to the original habitat. The main contribution of this study is that it experimentally documented the variability in the site fidelity of H. occidualis within multiple definitive host species and determined that site fidelity is not as strongly conserved in this genus as suggested previously. Additionally, this work suggests that the variation in site selection in different host species could lead to speciation of the parasites.

  17. Toxoplasma Effector MAF1 Mediates Recruitment of Host Mitochondria and Impacts the Host Response

    PubMed Central

    Pernas, Lena; Adomako-Ankomah, Yaw; Shastri, Anjali J.; Ewald, Sarah E.; Treeck, Moritz; Boyle, Jon P.; Boothroyd, John C.

    2014-01-01

    Recent information has revealed the functional diversity and importance of mitochondria in many cellular processes including orchestrating the innate immune response. Intriguingly, several infectious agents, such as Toxoplasma, Legionella, and Chlamydia, have been reported to grow within vacuoles surrounded by host mitochondria. Although many hypotheses have been proposed for the existence of host mitochondrial association (HMA), the causes and biological consequences of HMA have remained unanswered. Here we show that HMA is present in type I and III strains of Toxoplasma but missing in type II strains, both in vitro and in vivo. Analysis of F1 progeny from a type II×III cross revealed that HMA is a Mendelian trait that we could map. We use bioinformatics to select potential candidates and experimentally identify the polymorphic parasite protein involved, mitochondrial association factor 1 (MAF1). We show that introducing the type I (HMA+) MAF1 allele into type II (HMA−) parasites results in conversion to HMA+ and deletion of MAF1 in type I parasites results in a loss of HMA. We observe that the loss and gain of HMA are associated with alterations in the transcription of host cell immune genes and the in vivo cytokine response during murine infection. Lastly, we use exogenous expression of MAF1 to show that it binds host mitochondria and thus MAF1 is the parasite protein directly responsible for HMA. Our findings suggest that association with host mitochondria may represent a novel means by which Toxoplasma tachyzoites manipulate the host. The existence of naturally occurring HMA+ and HMA− strains of Toxoplasma, Legionella, and Chlamydia indicates the existence of evolutionary niches where HMA is either advantageous or disadvantageous, likely reflecting tradeoffs in metabolism, immune regulation, and other functions of mitochondria. PMID:24781109

  18. Water loss from olivine hosted melt inclusions

    NASA Astrophysics Data System (ADS)

    Chen, Y.; Provost, A.; Schiano, P.; Cluzel, N.

    2009-12-01

    Water content in melt inclusions has long been used as an important index for the water content of the hosting magma. However, many studies have shown that post-entrapment diffusive re-equilibration can affect the water content of melt inclusions. This process must be considered when using melt inclusions to infer water content of the hosting magma. Theoretical model on the diffusive re-equilibration between melt inclusions and external melts showed that the re-equilibration rate depends on the diffusivity of the re-equilibrating species in the host mineral, the partition coefficient of this species between the host mineral and melt, and the geometry of the melt inclusion and host mineral. The water diffusivity in olivine and water partition coefficient between melt and olivine have been measured by recent studies, therefore the diffusive re-equilibration model can be tested by experiments. In this study, we carried out in-situ Fourier transform infrared spectroscopy (FTIR) measurements on the water content of olivine hosted melt inclusions at high temperatures. Initial water content of the melt inclusions is about 4 wt%. A heating stage system is combined with a microscope FTIR and the absorption spectrum through the olivine and melt inclusion is repeatedly measured. Although the absorption band at around 3540 cm-1 has not be calibrated at high temperatures, it is assumed that the absorbance is linearly related to the total water concentration in the melt inclusion, and the relative water content can be inferred. Cautions have been exercised to maintain a consistent measurement spot such that the thickness of the melt inclusion within the beam path did not change significantly during each experiment. Oxygen fugacity in the heating stage is controlled by Zr purified Ar gas to be about 7 logarithm units below the QFM buffer and about 1 logarithm unit above the QIF buffer at 1473 K. Preliminary results showed that at 1430 and 1581 K, the total water content of the

  19. The monogenean parasite fauna of cichlids: a potential tool for host biogeography.

    PubMed

    Pariselle, Antoine; Boeger, Walter A; Snoeks, Jos; Bilong Bilong, Charles F; Morand, Serge; Vanhove, Maarten P M

    2011-01-01

    We discuss geographical distribution and phylogeny of Dactylogyridea (Monogenea) parasitizing Cichlidae to elucidate their hosts' history. Although mesoparasitic Monogenea (Enterogyrus spp.) show typical vicariant distribution, ectoparasitic representatives from different continents are not considered sister taxa, hence their distribution cannot result from vicariance alone. Because of the close host-parasite relationship, this might indicate that present-day cichlid distribution may also reflect dispersal through coastal or brackish waters. Loss of ectoparasites during transoceanic migration, followed by lateral transfer from other fish families might explain extant host-parasite associations. Because of its mesoparasitic nature, hence not subject to salinity variations of the host's environment, Enterogyrus could have survived marine migrations, intolerable for ectoparasites. Host-switches and salinity transitions may be invoked to explain the pattern revealed by a preliminary morphological phylogeny of monogenean genera from Cichlidae and other selected Monogenea genera, rendering the parasite distribution explicable under both vicariance and dispersal. Testable hypotheses are put forward in this parasitological approach to cichlid biogeography. Along with more comprehensive in-depth morphological phylogeny, comparison with molecular data, clarifying dactylogyridean evolution on different continents and from various fish families, and providing temporal information on host-parasite history, are needed to discriminate between the possible scenarios.

  20. Genetic diversity and distribution patterns of host insects of Caterpillar Fungus Ophiocordyceps sinensis in the Qinghai-Tibet Plateau.

    PubMed

    Quan, Qing-Mei; Chen, Ling-Ling; Wang, Xi; Li, Shan; Yang, Xiao-Ling; Zhu, Yun-Guo; Wang, Mu; Cheng, Zhou

    2014-01-01

    The caterpillar fungus Ophiocordyceps sinensis is one of the most valuable medicinal fungi in the world, and it requires host insects in family Hepialidae (Lepidoptera) to complete its life cycle. However, the genetic diversity and phylogeographic structures of the host insects remain to be explored. We analyzed the genetic diversity and temporal and spatial distribution patterns of genetic variation of the host insects throughout the O. sinensis distribution. Abundant haplotype and nucleotide diversity mainly existed in the areas of Nyingchi, ShangriLa, and around the edge of the Qinghai-Tibet Plateau, where are considered as the diversity center or micro-refuges of the host insects of O. sinensis. However, there was little genetic variation among host insects from 72.1% of all populations, indicating that the host species composition might be relatively simple in large-scale O. sinensis populations. All host insects are monophyletic except for those from four O. sinensis populations around Qinghai Lake. Significant phylogeographic structure (NST>GST, P<0.05) was revealed for the monophyletic host insects, and the three major phylogenetic groups corresponded with specific geographical areas. The divergence of most host insects was estimated to have occurred at ca. 3.7 Ma, shortly before the rapid uplift of the QTP. The geographical distribution and star-like network of the haplotypes implied that most host insects were derived from the relicts of a once-widespread host that subsequently became fragmented. Neutrality tests, mismatch distribution analysis, and expansion time estimation confirmed that most host insects presented recent demographic expansions that began ca. 0.118 Ma in the late Pleistocene. Therefore, the genetic diversity and distribution of the present-day insects should be attributed to effects of the Qinghai-Tibet Plateau uplift and glacial advance/retreat cycles during the Quaternary ice age. These results provide valuable information to guide

  1. Genetic diversity and distribution patterns of host insects of Caterpillar Fungus Ophiocordyceps sinensis in the Qinghai-Tibet Plateau.

    PubMed

    Quan, Qing-Mei; Chen, Ling-Ling; Wang, Xi; Li, Shan; Yang, Xiao-Ling; Zhu, Yun-Guo; Wang, Mu; Cheng, Zhou

    2014-01-01

    The caterpillar fungus Ophiocordyceps sinensis is one of the most valuable medicinal fungi in the world, and it requires host insects in family Hepialidae (Lepidoptera) to complete its life cycle. However, the genetic diversity and phylogeographic structures of the host insects remain to be explored. We analyzed the genetic diversity and temporal and spatial distribution patterns of genetic variation of the host insects throughout the O. sinensis distribution. Abundant haplotype and nucleotide diversity mainly existed in the areas of Nyingchi, ShangriLa, and around the edge of the Qinghai-Tibet Plateau, where are considered as the diversity center or micro-refuges of the host insects of O. sinensis. However, there was little genetic variation among host insects from 72.1% of all populations, indicating that the host species composition might be relatively simple in large-scale O. sinensis populations. All host insects are monophyletic except for those from four O. sinensis populations around Qinghai Lake. Significant phylogeographic structure (NST>GST, P<0.05) was revealed for the monophyletic host insects, and the three major phylogenetic groups corresponded with specific geographical areas. The divergence of most host insects was estimated to have occurred at ca. 3.7 Ma, shortly before the rapid uplift of the QTP. The geographical distribution and star-like network of the haplotypes implied that most host insects were derived from the relicts of a once-widespread host that subsequently became fragmented. Neutrality tests, mismatch distribution analysis, and expansion time estimation confirmed that most host insects presented recent demographic expansions that began ca. 0.118 Ma in the late Pleistocene. Therefore, the genetic diversity and distribution of the present-day insects should be attributed to effects of the Qinghai-Tibet Plateau uplift and glacial advance/retreat cycles during the Quaternary ice age. These results provide valuable information to guide

  2. Modeling Systems-Level Regulation of Host Immune Responses

    PubMed Central

    Thakar, Juilee; Pilione, Mylisa; Kirimanjeswara, Girish; Harvill, Eric T; Albert, Réka

    2007-01-01

    Many pathogens are able to manipulate the signaling pathways responsible for the generation of host immune responses. Here we examine and model a respiratory infection system in which disruption of host immune functions or of bacterial factors changes the dynamics of the infection. We synthesize the network of interactions between host immune components and two closely related bacteria in the genus Bordetellae. We incorporate existing experimental information on the timing of immune regulatory events into a discrete dynamic model, and verify the model by comparing the effects of simulated disruptions to the experimental outcome of knockout mutations. Our model indicates that the infection time course of both Bordetellae can be separated into three distinct phases based on the most active immune processes. We compare and discuss the effect of the species-specific virulence factors on disrupting the immune response during their infection of naive, antibody-treated, diseased, or convalescent hosts. Our model offers predictions regarding cytokine regulation, key immune components, and clearance of secondary infections; we experimentally validate two of these predictions. This type of modeling provides new insights into the virulence, pathogenesis, and host adaptation of disease-causing microorganisms and allows systems-level analysis that is not always possible using traditional methods. PMID:17559300

  3. Parallel Exploitation of Diverse Host Nutrients Enhances Salmonella Virulence

    PubMed Central

    Steeb, Benjamin; Claudi, Beatrice; Burton, Neil A.; Tienz, Petra; Schmidt, Alexander; Farhan, Hesso; Mazé, Alain; Bumann, Dirk

    2013-01-01

    Pathogen access to host nutrients in infected tissues is fundamental for pathogen growth and virulence, disease progression, and infection control. However, our understanding of this crucial process is still rather limited because of experimental and conceptual challenges. Here, we used proteomics, microbial genetics, competitive infections, and computational approaches to obtain a comprehensive overview of Salmonella nutrition and growth in a mouse typhoid fever model. The data revealed that Salmonella accessed an unexpectedly diverse set of at least 31 different host nutrients in infected tissues but the individual nutrients were available in only scarce amounts. Salmonella adapted to this situation by expressing versatile catabolic pathways to simultaneously exploit multiple host nutrients. A genome-scale computational model of Salmonella in vivo metabolism based on these data was fully consistent with independent large-scale experimental data on Salmonella enzyme quantities, and correctly predicted 92% of 738 reported experimental mutant virulence phenotypes, suggesting that our analysis provided a comprehensive overview of host nutrient supply, Salmonella metabolism, and Salmonella growth during infection. Comparison of metabolic networks of other pathogens suggested that complex host/pathogen nutritional interfaces are a common feature underlying many infectious diseases. PMID:23633950

  4. Hosts and parasites as aliens.

    PubMed

    Taraschewski, H

    2006-06-01

    Over the past decades, various free-living animals (hosts) and their parasites have invaded recipient areas in which they had not previously occurred, thus gaining the status of aliens or exotics. In general this happened to a low extent for hundreds of years. With variable frequency, invasions have been followed by the dispersal and establishment of non-indigenous species, whether host or parasite. In the literature thus far, colonizations by both hosts and parasites have not been treated and reviewed together, although both are usually interwoven in various ways. As to those factors permitting invasive success and colonization strength, various hypotheses have been put forward depending on the scientific background of respective authors and on the conspicuousness of certain invasions. Researchers who have tried to analyse characteristic developmental patterns, the speed of dispersal or the degree of genetic divergence in populations of alien species have come to different conclusions. Among parasitologists, the applied aspects of parasite invasions, such as the negative effects on economically important hosts, have long been at the centre of interest. In this contribution, invasions by hosts as well as parasites are considered comparatively, revealing many similarities and a few differences. Two helminths, the liver fluke, Fasciola hepatica, of cattle and sheep and the swimbladder nematode, Anguillicola crassus, of eels are shown to be useful as model parasites for the study of animal invasions and environmental global change. Introductions of F. hepatica have been associated with imports of cattle or other grazing animals. In various target areas, susceptible lymnaeid snails serving as intermediate hosts were either naturally present and/or were introduced from the donor continent of the parasite (Europe) and/or from other regions which were not within the original range of the parasite, partly reflecting progressive stages of a global biota change. In several

  5. How do ectoparasitic nycteribiids locate their bat hosts?

    PubMed

    Lourenço, S I; Palmeirim, J M

    2008-09-01

    Nycteribiids (Diptera: Nycteribiidae) are specific haematophagous ectoparasites of bats, which spend nearly all their adult lives on hosts. However, females have to leave bats to deposit their larva on the walls of the roosts, where they later emerge as adult flies. Nycteribiids had thus to evolve efficient sensorial mechanisms to locate hosts from a distance. We studied the sensory cues involved in this process, experimentally testing the role of specific host odours, and general cues such as carbon dioxide, body heat, and vibrations. As models we used two nycteribiids (Penicillidia conspicua and Penicillidia dufourii) and their primary bat hosts (Miniopterus schreibersii and Myotis myotis, respectively). Carbon dioxide was the most effective cue activating and orientating the responses of nycteribiids, followed by body heat and body odours. They also responded to vibration, but did not orientate to its source. In addition, sensory cues combined (carbon dioxide and body heat) were more effective in orientating nycteribiids than either cue delivered alone. Results suggest that nycteribids have some capacity to distinguish specific hosts from a distance, probably through their specific body odours. However, the strong reliance of nycteribiids on cues combined indicates that they follow these to orientate to nearby multispecies bat clusters, where the chances of finding their primary hosts are high. The combination of sensory cues seems therefore an effective strategy used by nycteribiids to locate bat hosts at a distance.

  6. Host stress hormones alter vector feeding preferences, success, and productivity.

    PubMed

    Gervasi, Stephanie S; Burkett-Cadena, Nathan; Burgan, Sarah C; Schrey, Aaron W; Hassan, Hassan K; Unnasch, Thomas R; Martin, Lynn B

    2016-08-17

    Stress hormones might represent a key link between individual-level infection outcome, population-level parasite transmission, and zoonotic disease risk. Although the effects of stress on immunity are well known, stress hormones could also affect host-vector interactions via modification of host behaviours or vector-feeding patterns and subsequent reproductive success. Here, we experimentally manipulated songbird stress hormones and examined subsequent feeding preferences, feeding success, and productivity of mosquito vectors in addition to defensive behaviours of hosts. Despite being more defensive, birds with elevated stress hormone concentrations were approximately twice as likely to be fed on by mosquitoes compared to control birds. Moreover, stress hormones altered the relationship between the timing of laying and clutch size in blood-fed mosquitoes. Our results suggest that host stress could affect the transmission dynamics of vector-borne parasites via multiple pathways. PMID:27512147

  7. Sticklebacks as model hosts in ecological and evolutionary parasitology.

    PubMed

    Barber, Iain

    2013-11-01

    The three-spined stickleback is a small teleost fish, native to coastal regions of the Northern Hemisphere, which has emerged as a key model organism in evolutionary biology and ecology. Sticklebacks possess a well-documented and experimentally amenable parasite fauna, and are well suited to both laboratory and field parasitological investigation. As a consequence, sticklebacks have been extensively used as model hosts in studies of host-parasite interactions, and these studies have provided considerable insight into the roles of parasites in ecology and evolutionary biology. In this review, I discuss key advances in our understanding of host-parasite interactions that have arisen from studies involving stickleback hosts, highlight areas of current research activity, and identify potentially promising areas for future research.

  8. Asteroseismology of Exoplanet Host Stars

    NASA Astrophysics Data System (ADS)

    Kayhan, Cenk; Çelik Orhan, Zeynep; Yildiz, Mutlu

    2016-07-01

    Exoplanet studies are one of the most interesting and attractive topics in astrophysics. Besides of ground-based observations, Kepler and CoRoT space missions improved our knowledge by providing unprecedented data of exoplanets and host stars. Precise determination of basic properties of planets depends on how we accurately determine fundamental properties of host stars. Asteroseismology is a powerful tool to study stellar structure and evolution and provides us radius, mass and age of the host stars. In this study, we construct stellar interior models of these stars with the MESA evolution code and compare model frequencies with the oscillation frequencies derived from Kepler data. Then, we obtain fundamental parameters of the host stars. Finally, fundamental parameters of exoplanets are reevaluated.

  9. Stennis hosts 2010 Special Olympics

    NASA Technical Reports Server (NTRS)

    2010-01-01

    B.J. Matherne, 27, of Gulfport, scores a soccer goal during one of the 2010 Special Olympic games at NASA's John C. Stennis Space Center on March 27. Stennis serves as an annual host for the special needs event. Each year, local, regional and national Special Olympics events are hosted in more than 150 countries for persons with special needs. An international Special Olympics competition is held every two years.

  10. The role of epigenetics in host-parasite coevolution: lessons from the model host insects Galleria mellonella and Tribolium castaneum.

    PubMed

    Vilcinskas, Andreas

    2016-08-01

    Recent studies addressing experimental host-parasite coevolution and transgenerational immune priming in insects provide evidence for heritable shifts in host resistance or parasite virulence. These rapid reciprocal adaptations may thus be transferred to offspring generations by either genetic changes or mechanisms that do not involve changes in the germline DNA sequence. Epigenetic inheritance refers to changes in gene expression that are heritable across generations and mediated by epigenetic modifications passed from parents to offspring. Highlighting the role of epigenetics in host-parasite coevolution, this review discusses the involvement of DNA methylation, histone acetylation/deacetylation and microRNAs in the interactions between bacterial or fungal parasites and model host insects such as the greater wax moth Galleria mellonella and the red flour beetle Tribolium castaneum. These epigenetic mechanisms are thought to participate in generation-spanning transcriptional reprogramming in the host insect, often linking immunity with developmentally related gene expression and contributing to the heredity of acquired adaptations. It is proposed that the interactions during host-parasite coevolution can therefore be expanded beyond reciprocal genetic changes to include reciprocal epigenetic changes. Epigenetics is thus a promising and prospering field in the context of host-parasite coevolution. PMID:27341739

  11. The role of epigenetics in host-parasite coevolution: lessons from the model host insects Galleria mellonella and Tribolium castaneum.

    PubMed

    Vilcinskas, Andreas

    2016-08-01

    Recent studies addressing experimental host-parasite coevolution and transgenerational immune priming in insects provide evidence for heritable shifts in host resistance or parasite virulence. These rapid reciprocal adaptations may thus be transferred to offspring generations by either genetic changes or mechanisms that do not involve changes in the germline DNA sequence. Epigenetic inheritance refers to changes in gene expression that are heritable across generations and mediated by epigenetic modifications passed from parents to offspring. Highlighting the role of epigenetics in host-parasite coevolution, this review discusses the involvement of DNA methylation, histone acetylation/deacetylation and microRNAs in the interactions between bacterial or fungal parasites and model host insects such as the greater wax moth Galleria mellonella and the red flour beetle Tribolium castaneum. These epigenetic mechanisms are thought to participate in generation-spanning transcriptional reprogramming in the host insect, often linking immunity with developmentally related gene expression and contributing to the heredity of acquired adaptations. It is proposed that the interactions during host-parasite coevolution can therefore be expanded beyond reciprocal genetic changes to include reciprocal epigenetic changes. Epigenetics is thus a promising and prospering field in the context of host-parasite coevolution.

  12. Multidimensionality in host manipulation mimicked by serotonin injection

    PubMed Central

    Perrot-Minnot, Marie-Jeanne; Sanchez-Thirion, Kevin; Cézilly, Frank

    2014-01-01

    Manipulative parasites often alter the phenotype of their hosts along multiple dimensions. ‘Multidimensionality’ in host manipulation could consist in the simultaneous alteration of several physiological pathways independently of one another, or proceed from the disruption of some key physiological parameter, followed by a cascade of effects. We compared multidimensionality in ‘host manipulation’ between two closely related amphipods, Gammarus fossarum and Gammarus pulex, naturally and experimentally infected with Pomphorhynchus laevis (Acanthocephala), respectively. To that end, we calculated in each host–parasite association the effect size of the difference between infected and uninfected individuals for six different traits (activity, phototaxis, geotaxis, attraction to conspecifics, refuge use and metabolic rate). The effects sizes were highly correlated between host–parasite associations, providing evidence for a relatively constant ‘infection syndrome’. Using the same methodology, we compared the extent of phenotypic alterations induced by an experimental injection of serotonin (5-HT) in uninfected G. pulex to that induced by experimental or natural infection with P. laevis. We observed a significant correlation between effect sizes across the six traits, indicating that injection with 5-HT can faithfully mimic the ‘infection syndrome’. This is, to our knowledge, the first experimental evidence that multidimensionality in host manipulation can proceed, at least partly, from the disruption of some major physiological mechanism. PMID:25339729

  13. Species diversity reduces parasite infection through cross-generational effects on host abundance.

    PubMed

    Johnson, Pieter T J; Preston, Daniel L; Hoverman, Jason T; Henderson, Jeremy S; Paull, Sara H; Richgels, Katherine L D; Redmond, Miranda D

    2012-01-01

    With growing interest in the effects of biodiversity on disease, there is a critical need for studies that empirically identify the mechanisms underlying the diversity-disease relationship. Here, we combined wetland surveys of host community structure with mechanistic experiments involving a multi-host parasite to evaluate competing explanations for the dilution effect. Sampling of 320 wetlands in California indicated that snail host communities were strongly nested, with competent hosts for the trematode Ribeiroia ondatrae predominating in low-richness assemblages and unsuitable hosts increasingly present in more diverse communities. Moreover, competent host density was negatively associated with increases in snail species richness. These patterns in host community assembly support a key prerequisite underlying the dilution effect. Results of multigenerational mesocosm experiments designed to mimic field-observed community assemblages allowed us to evaluate the relative importance of host density and diversity in influencing parasite infection success. Increases in snail species richness (from one to four species) had sharply negative effects on the density of infected hosts (-90% reduction). However, this effect was indirect; competition associated with non-host species led to a 95% reduction in host density (susceptible host regulation), owing primarily to a reduction in host reproduction. Among susceptible hosts, there were no differences in infection prevalence as a function of community structure, indicating a lack of support for a direct effect of diversity on infection (encounter reduction). In monospecific conditions, higher initial host densities increased infection among adult hosts; however, compensatory reproduction in the low-density treatments equalized the final number of infected hosts by the next generation, underscoring the relevance of multigenerational studies in understanding the dilution effect. These findings highlight the role of

  14. HPIDB - a unified resource for host-pathogen interactions

    PubMed Central

    2010-01-01

    Background Protein-protein interactions (PPIs) play a crucial role in initiating infection in a host-pathogen system. Identification of these PPIs is important for understanding the underlying biological mechanism of infection and identifying putative drug targets. Database resources for studying host-pathogen systems are scarce and are either host specific or dedicated to specific pathogens. Results Here we describe "HPIDB” a host-pathogen PPI database, which will serve as a unified resource for host-pathogen interactions. Specifically, HPIDB integrates experimental PPIs from several public databases into a single, non-redundant web accessible resource. The database can be searched with a variety of options such as sequence identifiers, symbol, taxonomy, publication, author, or interaction type. The output is provided in a tab delimited text file format that is compatible with Cytoscape, an open source resource for PPI visualization. HPIDB allows the user to search protein sequences using BLASTP to retrieve homologous host/pathogen sequences. For high-throughput analysis, the user can search multiple protein sequences at a time using BLASTP and obtain results in tabular and sequence alignment formats. The taxonomic categorization of proteins (bacterial, viral, fungi, etc.) involved in PPI enables the user to perform category specific BLASTP searches. In addition, a new tool is introduced, which allows searching for homologous host-pathogen interactions in the HPIDB database. Conclusions HPIDB is a unified, comprehensive resource for host-pathogen PPIs. The user interface provides new features and tools helpful for studying host-pathogen interactions. HPIDB can be accessed at http://agbase.msstate.edu/hpi/main.html. PMID:20946599

  15. Molecular evidence for host-adapted races of the fungal endophyte Epichloë bromicola after presumed host shifts.

    PubMed

    Brem, Dominik; Leuchtmann, Adrian

    2003-01-01

    Host shifts of plant-feeding insects and parasites promote adaptational changes that may result in the formation of host races, an assumed intermediate stage in sympatric speciation. Here, we report on genetically differentiated and host-adapted races of the fungal endophyte Epichloë bromicola, which presumably emerged after a shift from the grass Bromus erectus to other Bromus hosts. Fungi of the genus Epichloë (Ascomycota) and related anamorphs of Neotyphodium are widespread endophytes of cool-season grasses. Sexually reproducing strains sterilize the host by formation of external fruiting structures (stromata), whereas asexual strains are asymptomatic and transmitted via seeds. In E. bromicola, strains infecting B. erectus are sexual, and strains from two woodland species, B. benekenii and B. ramosus, are asexual and seed transmitted. Analyses of amplified fragment length polymorphism fingerprinting and of intron sequences of the tub2 and tef1 genes of 26 isolates from the three Bromus hosts collected at natural sites in Switzerland and nearby France demonstrated that isolates are genetically differentiated according to their host, indicating that E. bromicola does not form a single, randomly mating population. Phylogenetic analyses of sequence data did not unambiguously resolve the exact origin of asexual E. bromicola strains, but it is likely they arose from within sexual populations on B. erectus. Incongruence of trees derived from different genes may have resulted from recombination at some time in the recent history of host strains. Reciprocal inoculations of host plant seedlings showed that asexual isolates from B. benekenii and B. ramosus were incapable of infecting B. erectus, whereas the sexual isolates from B. erectus retained the assumed ancestral trait of broad compatibility with Bromus host seedlings. Because all isolates were interfertile in experimental crosses, asexual strains may not be considered independent biological species. We suggest

  16. Paratenic hosts as regular transmission route in the acanthocephalan Pomphorhynchus laevis: potential implications for food webs.

    PubMed

    Médoc, Vincent; Rigaud, Thierry; Motreuil, Sébastien; Perrot-Minnot, Marie-Jeanne; Bollache, Loïc

    2011-10-01

    Although trophically transmitted parasites are recognized to strongly influence food-web dynamics through their ability to manipulate host phenotype, our knowledge of their host spectrum is often imperfect. This is particularly true for the facultative paratenic hosts, which receive little interest. We investigated the occurrence and significance both in terms of ecology and evolution of paratenic hosts in the life cycle of the fish acanthocephalan Pomphorhynchus laevis. This freshwater parasite uses amphipods as intermediate hosts and cyprinids and salmonids as definitive hosts. Within a cohort of parasite larvae, usually reported in amphipod intermediate hosts, more than 90% were actually hosted by small-sized fish. We demonstrated experimentally, using one of these fish, that they get infected through the consumption of parasitized amphipods and contribute to the parasite's transmission to a definitive host, hence confirming their paratenic host status. A better knowledge of paratenic host spectrums could help us to understand the fine tuning of transmission strategies, to better estimate parasite biomass, and could improve our perception of parasite subwebs in terms of host-parasite and predator-parasite links.

  17. Midgut microbiota and host immunocompetence underlie Bacillus thuringiensis killing mechanism.

    PubMed

    Caccia, Silvia; Di Lelio, Ilaria; La Storia, Antonietta; Marinelli, Adriana; Varricchio, Paola; Franzetti, Eleonora; Banyuls, Núria; Tettamanti, Gianluca; Casartelli, Morena; Giordana, Barbara; Ferré, Juan; Gigliotti, Silvia; Ercolini, Danilo; Pennacchio, Francesco

    2016-08-23

    Bacillus thuringiensis is a widely used bacterial entomopathogen producing insecticidal toxins, some of which are expressed in insect-resistant transgenic crops. Surprisingly, the killing mechanism of B. thuringiensis remains controversial. In particular, the importance of the septicemia induced by the host midgut microbiota is still debated as a result of the lack of experimental evidence obtained without drastic manipulation of the midgut and its content. Here this key issue is addressed by RNAi-mediated silencing of an immune gene in a lepidopteran host Spodoptera littoralis, leaving the midgut microbiota unaltered. The resulting cellular immunosuppression was characterized by a reduced nodulation response, which was associated with a significant enhancement of host larvae mortality triggered by B. thuringiensis and a Cry toxin. This was determined by an uncontrolled proliferation of midgut bacteria, after entering the body cavity through toxin-induced epithelial lesions. Consequently, the hemolymphatic microbiota dramatically changed upon treatment with Cry1Ca toxin, showing a remarkable predominance of Serratia and Clostridium species, which switched from asymptomatic gut symbionts to hemocoelic pathogens. These experimental results demonstrate the important contribution of host enteric flora in B. thuringiensis-killing activity and provide a sound foundation for developing new insect control strategies aimed at enhancing the impact of biocontrol agents by reducing the immunocompetence of the host. PMID:27506800

  18. Host receptors for bacteriophage adsorption.

    PubMed

    Bertozzi Silva, Juliano; Storms, Zachary; Sauvageau, Dominic

    2016-02-01

    The adsorption of bacteriophages (phages) onto host cells is, in all but a few rare cases, a sine qua non condition for the onset of the infection process. Understanding the mechanisms involved and the factors affecting it is, thus, crucial for the investigation of host-phage interactions. This review provides a survey of the phage host receptors involved in recognition and adsorption and their interactions during attachment. Comprehension of the whole infection process, starting with the adsorption step, can enable and accelerate our understanding of phage ecology and the development of phage-based technologies. To assist in this effort, we have established an open-access resource--the Phage Receptor Database (PhReD)--to serve as a repository for information on known and newly identified phage receptors. PMID:26755501

  19. Evolution of host specificity drives reproductive isolation among RNA viruses.

    PubMed

    Duffy, Siobain; Burch, Christina L; Turner, Paul E

    2007-11-01

    Ecological speciation hypotheses claim that assortative mating evolves as a consequence of divergent natural selection for ecologically important traits. Reproductive isolation is expected to be particularly likely to evolve by this mechanism in species such as phytophagous insects that mate in the habitats in which they eat. We tested this expectation by monitoring the evolution of reproductive isolation in laboratory populations of an RNA virus that undergoes genetic exchange only when multiple virus genotypes coinfect the same host. We subjected four populations of the RNA bacteriophage phi6 to 150 generations of natural selection on a novel host. Although there was no direct selection acting on host range in our experiment, three of the four populations lost the ability to infect one or more alternative hosts. In the most extreme case, one of the populations evolved a host range that does not contain any of the hosts infectible by the wild-type phi6. Whole genome sequencing confirmed that the resulting reproductive isolation was due to a single nucleotide change, highlighting the ease with which an emerging RNA virus can decouple its evolutionary fate from that of its ancestor. Our results uniquely demonstrate the evolution of reproductive isolation in allopatric experimental populations. Furthermore, our data confirm the biological credibility of simple "no-gene" mechanisms of assortative mating, in which this trait arises as a pleiotropic effect of genes responsible for ecological adaptation.

  20. Restoring host-microbe homeostasis via selective chemoattraction of Tregs.

    PubMed

    Garlet, G P; Sfeir, C S; Little, S R

    2014-09-01

    The disruption of host-microbe homeostasis at the site of periodontal disease is considered a key factor for disease initiation and progress. While the downstream mechanisms responsible for the tissue damage per se are relatively well-known (involving various patterns of immune response operating toward periodontal tissue destruction), we are only beginning to understand the complexity of host-microbe interactions in the periodontal environment. Unfortunately, most of the research has been focused on the disruption of host-microbe homeostasis instead of focusing on the factors responsible for maintaining homeostasis. In this context, regulatory T-cells (Tregs) comprise a CD4+FOXp3 +T-cell subset with a unique ability to regulate other leukocyte functions to avoid excessive immune activation and its pathological consequences. Tregs act as critical determinants of host-microbe homeostasis, as well as determinants of a balanced host response after the disruption of host-microbe homeostasis by pathogens. In periodontitis, Tregs play a protective role, with their natural recruitment being responsible for conversion of active into inactive lesions. With controlled-release technology, it is now possible to achieve a selective chemoattraction of Tregs to periodontal tissues, attenuating experimental periodontitis evolution due to the local control of inflammatory immune response and the generation of a pro-reparative environment.

  1. Viral video: Live imaging of virus-host encounters

    NASA Astrophysics Data System (ADS)

    Son, Kwangmin; Guasto, Jeffrey S.; Cubillos-Ruiz, Andres; Chisholm, Sallie W.; Sullivan, Matthew B.; Stocker, Roman

    2014-11-01

    Viruses are non-motile infectious agents that rely on Brownian motion to encounter and subsequently adsorb to their hosts. Paradoxically, the viral adsorption rate is often reported to be larger than the theoretical limit imposed by the virus-host encounter rate, highlighting a major gap in the experimental quantification of virus-host interactions. Here we present the first direct quantification of the viral adsorption rate, obtained using live imaging of individual host cells and viruses for thousands of encounter events. The host-virus pair consisted of Prochlorococcus MED4, a 800 nm small non-motile bacterium that dominates photosynthesis in the oceans, and its virus PHM-2, a myovirus that has a 80 nm icosahedral capsid and a 200 nm long rigid tail. We simultaneously imaged hosts and viruses moving by Brownian motion using two-channel epifluorescent microscopy in a microfluidic device. This detailed quantification of viral transport yielded a 20-fold smaller adsorption efficiency than previously reported, indicating the need for a major revision in infection models for marine and likely other ecosystems.

  2. The Inflammasome in Host Defense

    PubMed Central

    Chen, Gang; Pedra, Joao H.F.

    2010-01-01

    Nod-like receptors have emerged as an important family of sensors in host defense. These receptors are expressed in macrophages, dendritic cells and monocytes and play an important role in microbial immunity. Some Nod-like receptors form the inflammasome, a protein complex that activates caspase-1 in response to several stimuli. Caspase-1 activation leads to processing and secretion of pro-inflammatory cytokines such as interleukin (IL)-1β and IL-18. Here, we discuss recent advances in the inflammasome field with an emphasis on host defense. We also compare differential requirements for inflammasome activation in dendritic cells, macrophages and monocytes. PMID:22315529

  3. History of graft-versus-host disease.

    PubMed

    Vriesendorp, Huib M; Heidt, Peter J

    2016-08-01

    Nuclear warfare at the end of World War II inspired Dick W. van Bekkum to study total-body irradiation (TBI) in animal models. After high-dose TBI, mice died from "primary disease" or bone marrow (BM) aplasia. Intravenous administration of allogeneic BM cells delayed mortality but did not prevent it. Initially the delayed deaths were said to be caused by "secondary disease," which was later renamed graft-versus-host disease (GvHD). GvHD is caused by donor T lymphocytes that destroy recipient cells in skin, intestinal mucosa, bile ducts, and lymph nodes. GvHD is opposed by host-versus-graft disease (HvGD), in which host T lymphocytes destroy the administered allogeneic BM cells, including the administered T lymphocytes of the BM donor. In 1960, van Bekkum became the director of the Radiobiological Institute of the Dutch Organization for Applied Scientific Research TNO, Rijswijk, The Netherlands, where he built a multidisciplinary team that defined the variables controlling the outcome of a BM transplant. The team published their early results in the Journal of Experimental Hematology [1981;9:904-916 and 1956;4:482-488]. Later, protocols were established for BM transplantation (BMT) in patients with severe combined immunodeficiency disease, leukemia, lymphoma, and other diseases of the hematopoietic system. This review honors the scientific contributions made by Dick van Bekkum and his team in defining the four dominant variables for improving the therapeutic ratio of allogeneic BMT and in fostering the international collaboration necessary to translate this knowledge into current clinical practice. PMID:27235758

  4. Host diversity begets parasite diversity: Bird final hosts and trematodes in snail intermediate hosts

    USGS Publications Warehouse

    Hechinger, R.F.; Lafferty, K.D.

    2005-01-01

    An unappreciated facet of biodiversity is that rich communities and high abundance may foster parasitism. For parasites that sequentially use different host species throughout complex life cycles, parasite diversity and abundance in 'downstream' hosts should logically increase with the diversity and abundance of 'upstream' hosts (which carry the preceding stages of parasites). Surprisingly, this logical assumption has little empirical support, especially regarding metazoan parasites. Few studies have attempted direct tests of this idea and most have lacked the appropriate scale of investigation. In two different studies, we used time-lapse videography to quantify birds at fine spatial scales, and then related bird communities to larval trematode communities in snail populations sampled at the same small spatial scales. Species richness, species heterogeneity and abundance of final host birds were positively correlated with species richness, species heterogeneity and abundance of trematodes in host snails. Such community-level interactions have rarely been demonstrated and have implications for community theory, epidemiological theory and ecosystem management. ?? 2005 The Royal Society.

  5. Selection by parasites may increase host recombination frequency.

    PubMed

    Fischer, O; Schmid-Hempel, P

    2005-06-22

    Meiotic recombination destroys successful genotypes and it is therefore thought to evolve only under a very limited set of conditions. Here, we experimentally show that recombination rates across two linkage groups of the host, the red flour beetle Tribolium castaneum, increase with exposure to the microsporidian parasite, Nosema whitei, particularly when parasites were allowed to coevolve with their hosts. Selection by randomly varied parasites resulted in smaller effects, while directional selection for insecticide resistance initially reduced recombination slightly. These results, at least tentatively, suggest that short-term benefits of recombination--and thus the evolution of sex--may be related to parasitism.

  6. Parasitism, host immune function, and sexual selection.

    PubMed

    Møller, A P; Christe, P; Lux, E

    1999-03-01

    Parasite-mediated sexual selection may arise as a consequence of 1) females avoiding mates with directly transmitted parasites, 2) females choosing less-parasitized males that provide parental care of superior quality, or 3) females choosing males with few parasites in order to obtain genes for parasite resistance in their offspring. Studies of specific host-parasite systems and comparative analyses have revealed both supportive and conflicting evidence for these hypotheses. A meta-analysis of the available evidence revealed a negative relationship between parasite load and the expression of male secondary sexual characters. Experimental studies yielded more strongly negative relationships than observations did, and the relationships were more strongly negative for ectoparasites than for endoparasites. There was no significant difference in the magnitude of the negative effect for species with and without male parental care, or between behavioral and morphological secondary sexual characters. There was a significant difference between studies based on host immune function and those based on parasite loads, with stronger effects for measures of immune function, suggesting that the many negative results from previous analyses of parasite-mediated sexual selection may be explained because relatively benign parasites were studied. The multivariate analyses demonstrating strong effect sizes of immune function in relation to the expression of secondary sexual characters, and for species with male parental care as compared to those without, suggest that parasite resistance may be a general determinant of parasite-mediated sexual selection. PMID:10081812

  7. Host Event Based Network Monitoring

    SciTech Connect

    Jonathan Chugg

    2013-01-01

    The purpose of INL’s research on this project is to demonstrate the feasibility of a host event based network monitoring tool and the effects on host performance. Current host based network monitoring tools work on polling which can miss activity if it occurs between polls. Instead of polling, a tool could be developed that makes use of event APIs in the operating system to receive asynchronous notifications of network activity. Analysis and logging of these events will allow the tool to construct the complete real-time and historical network configuration of the host while the tool is running. This research focused on three major operating systems commonly used by SCADA systems: Linux, WindowsXP, and Windows7. Windows 7 offers two paths that have minimal impact on the system and should be seriously considered. First is the new Windows Event Logging API, and, second, Windows 7 offers the ALE API within WFP. Any future work should focus on these methods.

  8. Undiscovered Bat Hosts of Filoviruses.

    PubMed

    Han, Barbara A; Schmidt, John Paul; Alexander, Laura W; Bowden, Sarah E; Hayman, David T S; Drake, John M

    2016-07-01

    Ebola and other filoviruses pose significant public health and conservation threats by causing high mortality in primates, including humans. Preventing future outbreaks of ebolavirus depends on identifying wildlife reservoirs, but extraordinarily high biodiversity of potential hosts in temporally dynamic environments of equatorial Africa contributes to sporadic, unpredictable outbreaks that have hampered efforts to identify wild reservoirs for nearly 40 years. Using a machine learning algorithm, generalized boosted regression, we characterize potential filovirus-positive bat species with estimated 87% accuracy. Our model produces two specific outputs with immediate utility for guiding filovirus surveillance in the wild. First, we report a profile of intrinsic traits that discriminates hosts from non-hosts, providing a biological caricature of a filovirus-positive bat species. This profile emphasizes traits describing adult and neonate body sizes and rates of reproductive fitness, as well as species' geographic range overlap with regions of high mammalian diversity. Second, we identify several bat species ranked most likely to be filovirus-positive on the basis of intrinsic trait similarity with known filovirus-positive bats. New bat species predicted to be positive for filoviruses are widely distributed outside of equatorial Africa, with a majority of species overlapping in Southeast Asia. Taken together, these results spotlight several potential host species and geographical regions as high-probability targets for future filovirus surveillance. PMID:27414412

  9. Biosignatures of Pathogen and Host

    SciTech Connect

    Fitch, J P; Chromy, B A; Forde, C E; Garcia, E; Gardner, S N; Gu, P P; Kuczmarksi, T A; Melius, C F; McCutchen-Maloney, S L; Milanovich, F P; Motin, V L; Ott, L L; Quong, A A; Quong, J N; Rocco, J M; Slezak, T R; Sokhansanj, B A; Vitalis, E A; Zemla, A T; McCready, P M

    2002-08-27

    In information theory, a signature is characterized by the information content as well as noise statistics of the communication channel. Biosignatures have analogous properties. A biosignature can be associated with a particular attribute of a pathogen or a host. However, the signature may be lost in backgrounds of similar or even identical signals from other sources. In this paper, we highlight statistical and signal processing challenges associated with identifying good biosignatures for pathogens in host and other environments. In some cases it may be possible to identify useful signatures of pathogens through indirect but amplified signals from the host. Discovery of these signatures requires new approaches to modeling and data interpretation. For environmental biosignal collections, it is possible to use signal processing techniques from other applications (e.g., synthetic aperture radar) to track the natural progression of microbes over large areas. We also present a computer-assisted approach to identify unique nucleic-acid based microbial signatures. Finally, an understanding of host-pathogen interactions will result in better detectors as well as opportunities in vaccines and therapeutics.

  10. AVTC Hosts TechnoCamp

    ERIC Educational Resources Information Center

    Miner, Brenda

    2006-01-01

    The Area Vo-Tech Center (AVTC) in Russellville, Arkansas, recently hosted its first TechnoCamp to encourage enrollment based on the aptitude and interest level of the students enrolling in the various programs. The center currently offers student enrollment in auto technology, computer engineering, cosmetology, construction technology, drafting…

  11. Undiscovered Bat Hosts of Filoviruses

    PubMed Central

    Schmidt, John Paul; Alexander, Laura W.; Bowden, Sarah E.; Hayman, David T. S.; Drake, John M.

    2016-01-01

    Ebola and other filoviruses pose significant public health and conservation threats by causing high mortality in primates, including humans. Preventing future outbreaks of ebolavirus depends on identifying wildlife reservoirs, but extraordinarily high biodiversity of potential hosts in temporally dynamic environments of equatorial Africa contributes to sporadic, unpredictable outbreaks that have hampered efforts to identify wild reservoirs for nearly 40 years. Using a machine learning algorithm, generalized boosted regression, we characterize potential filovirus-positive bat species with estimated 87% accuracy. Our model produces two specific outputs with immediate utility for guiding filovirus surveillance in the wild. First, we report a profile of intrinsic traits that discriminates hosts from non-hosts, providing a biological caricature of a filovirus-positive bat species. This profile emphasizes traits describing adult and neonate body sizes and rates of reproductive fitness, as well as species’ geographic range overlap with regions of high mammalian diversity. Second, we identify several bat species ranked most likely to be filovirus-positive on the basis of intrinsic trait similarity with known filovirus-positive bats. New bat species predicted to be positive for filoviruses are widely distributed outside of equatorial Africa, with a majority of species overlapping in Southeast Asia. Taken together, these results spotlight several potential host species and geographical regions as high-probability targets for future filovirus surveillance. PMID:27414412

  12. Symbiont-mediated functions in insect hosts

    PubMed Central

    Su, Qi; Zhou, Xiaomao; Zhang, Youjun

    2013-01-01

    The bacterial endosymbionts occur in a diverse array of insect species and are usually rely within the vertical transmission from mothers to offspring. In addition to primary symbionts, plant sap-sucking insects may also harbor several diverse secondary symbionts. Bacterial symbionts play a prominent role in insect nutritional ecology by aiding in digestion of food or supplementing nutrients that insect hosts can’t obtain sufficient amounts from a restricted diet of plant phloem. Currently, several other ecologically relevant traits mediated by endosymbionts are being investigated, including defense toward pathogens and parasites, adaption to environment, influences on insect-plant interactions, and impact of population dynamics. Here, we review recent theoretical predictions and experimental observations of these traits mediated by endosymbionts and suggest that clarifying the roles of symbiotic microbes may be important to offer insights for ameliorating pest invasiveness or impact. PMID:23710278

  13. Evaluating the within-host fitness effects of mutations fixed during virus adaptation to different ecotypes of a new host.

    PubMed

    Hillung, Julia; Cuevas, José M; Elena, Santiago F

    2015-08-19

    The existence of genetic variation for resistance in host populations is assumed to be essential to the spread of an emerging virus. Models predict that the rate of spread slows down with the increasing frequency and higher diversity of resistance alleles in the host population. We have been using the experimental pathosystem Arabidopsis thaliana-tobacco etch potyvirus (TEV) to explore the interplay between genetic variation in host's susceptibility and virus diversity. We have recently shown that TEV populations evolving in A. thaliana ecotypes that differ in susceptibility to infection gained within-host fitness, virulence and infectivity in a manner compatible with a gene-for-gene model of host-parasite interactions: hard-to-infect ecotypes were infected by generalist viruses, whereas easy-to-infect ecotypes were infected by every virus. We characterized the genomes of the evolved viruses and found cases of host-driven convergent mutations. To gain further insights in the mechanistic basis of this gene-for-gene model, we have generated all viral mutations individually as well as in specific combinations and tested their within-host fitness effects across ecotypes. Most of these mutations were deleterious or neutral in their local ecotype and only a very reduced number had a host-specific beneficial effect. We conclude that most of the mutations fixed during the evolution experiment were so by drift or by selective sweeps along with the selected driver mutation. In addition, we evaluated the ruggedness of the underlying adaptive fitness landscape and found that mutational effects were mostly multiplicative, with few cases of significant epistasis. PMID:26150658

  14. Host size and spatiotemporal patterns mediate the coexistence of specialist parasitoids.

    PubMed

    Pekas, Apostolos; Tena, Alejandro; Harvey, Jeffrey A; Garcia-Mari, Ferran; Frago, Enric

    2016-05-01

    Many insect parasitoids are highly specialized and thus develop on only one or a few related host species, yet some hosts are attacked by many different parasitoid species in nature. For this reason, they have been often used to examine the consequences of competitive interactions. Hosts represent limited resources for larval parasitoid development and thus one competitor usually excludes all others. Although parasitoid competition has been debated and studied over the past several decades, understanding the factors that allow for coexistence among species sharing the same host in the field remains elusive. Parasitoids may be able to coexist on the same host species if they partition host resources according to size, age, or stage, or if their dynamics vary at spatial and temporal scales. One area that has thus far received little experimental attention is if competition can alter host usage strategies in parasitoids that in the absence of competitors attack hosts of the same size in the field. Here, we test this hypothesis with two parasitoid species in the genus Aphytis, both of which are specialized on the citrus pest California red scale Aonidiella aurantii. These parasitoids prefer large scales as hosts and yet coexist in sympatry in eastern parts of Spain. Parasitoids and hosts were sampled in 12 replicated orange groves. When host exploitation by the stronger competitor, A. melinus, was high the poorer competitor, A. chrysomphali, changed its foraging strategy to prefer alternative plant substrates where it parasitized hosts of smaller size. Consequently, the inferior parasitoid species shifted both its habitat and host size as a result of competition. Our results suggest that density-dependent size-mediated asymmetric competition is the likely mechanism allowing for the coexistence of these two species, and that the use of suboptimal (small) hosts can be advantageous under conditions imposed by competition where survival in higher quality larger hosts may

  15. Host size and spatiotemporal patterns mediate the coexistence of specialist parasitoids.

    PubMed

    Pekas, Apostolos; Tena, Alejandro; Harvey, Jeffrey A; Garcia-Mari, Ferran; Frago, Enric

    2016-05-01

    Many insect parasitoids are highly specialized and thus develop on only one or a few related host species, yet some hosts are attacked by many different parasitoid species in nature. For this reason, they have been often used to examine the consequences of competitive interactions. Hosts represent limited resources for larval parasitoid development and thus one competitor usually excludes all others. Although parasitoid competition has been debated and studied over the past several decades, understanding the factors that allow for coexistence among species sharing the same host in the field remains elusive. Parasitoids may be able to coexist on the same host species if they partition host resources according to size, age, or stage, or if their dynamics vary at spatial and temporal scales. One area that has thus far received little experimental attention is if competition can alter host usage strategies in parasitoids that in the absence of competitors attack hosts of the same size in the field. Here, we test this hypothesis with two parasitoid species in the genus Aphytis, both of which are specialized on the citrus pest California red scale Aonidiella aurantii. These parasitoids prefer large scales as hosts and yet coexist in sympatry in eastern parts of Spain. Parasitoids and hosts were sampled in 12 replicated orange groves. When host exploitation by the stronger competitor, A. melinus, was high the poorer competitor, A. chrysomphali, changed its foraging strategy to prefer alternative plant substrates where it parasitized hosts of smaller size. Consequently, the inferior parasitoid species shifted both its habitat and host size as a result of competition. Our results suggest that density-dependent size-mediated asymmetric competition is the likely mechanism allowing for the coexistence of these two species, and that the use of suboptimal (small) hosts can be advantageous under conditions imposed by competition where survival in higher quality larger hosts may

  16. Multiple effects of host-species diversity on coexisting host-specific and host-opportunistic microbes.

    PubMed

    Kedem, Hadar; Cohen, Carmit; Messika, Irit; Einav, Monica; Pilosof, Shai; Hawlena, Hadas

    2014-05-01

    While host-species diversity often influences microbial prevalence, there may be multiple mechanisms causing such effects that may also depend on the foraging strategy of the microbes. We employed a natural gradient of rodent-species richness to examine competing hypotheses describing possible mechanisms mediating the relationship between host-species richness and the prevalence of the most dominant microbes, along with microbe specificity to the different rodent host species. We sampled blood from three gerbil species in plots differing in terms of the proportion of the different species and screened for the most dominant bacteria. Two dominant bacterial lineages were detected: host-specific bacteria and host-opportunistic bacteria. Using a model selection approach, we detected evidence for both direct and indirect effects of host-species richness on the prevalence of these bacteria. Infection probability of the host-specific lineage was lower in richer host communities, most likely due to increased frequency and density of the least suitable host species. In contrast, field observations suggest that the effect of host-species richness on infection probability of the opportunistic lineage was both direct and indirect, mostly mediated by changes in flea densities on the host and by the presence of the host-specific lineage. Our results thus suggest that host-species richness has multiple effects on microbial prevalence, depending on the degree of host-specificity of the microbe in question. PMID:25000749

  17. Location of Host and Host Habitat by Fruit Fly Parasitoids

    PubMed Central

    Quilici, Serge; Rousse, Pascal

    2012-01-01

    Augmentative releases of parasitoids may be a useful tool for the area-wide management of tephritid pests. The latter are parasitized by many wasp species, though only a few of them are relevant for augmentative biocontrol purposes. To date, nearly all the actual or potential biocontrol agents for such programs are egg or larval Opiinae parasitoids (Hymenoptera: Braconidae). Here, we review the literature published on their habitat and host location behavior, as well as the factors that modulate this behavior, which is assumed to be sequential; parasitoids forage first for the host habitat and then for the host itself. Parasitoids rely on chemical, visual, and mechanical stimuli, often strongly related to their ecology. Behavioral modulation factors include biotic and abiotic factors including learning, climatic conditions and physiological state of the insect. Finally, conclusions and perspectives for future research are briefly highlighted. A detailed knowledge of this behavior may be very useful for selecting the release sites for both inundative/augmentative releases of mass-reared parasitoids and inoculative releases for classical biocontrol. PMID:26466736

  18. Parasites, immunology of hosts, and host sexual selection.

    PubMed

    Møller, A P; Saino, N

    1994-12-01

    Parasite-mediated sexual selection is reviewed with special emphasis on the bird literature. Choosy females may benefit from choosing parasite-free mates if such males provide better parental care, do not transmit contagious parasites, or provide resistance genes to offspring. There is evidence in support of each of these mechanisms. The immunocompetence handicap hypothesis posits that secondary sexual characters reliably reveal the ability of males to resist parasites due to the immunosuppressive effects of testosterone and other biochemicals. Several aspects of these negative feedback mechanisms are supported by laboratory studies, but evidence from free-living animals is almost completely absent. Corticosterone rather than testosterone may potentially mediate the immunocompetence handicap mechanism. A simple version of the immunocompetence handicap is developed suggesting that body condition of male hosts is a sufficient mediator of the handicap mechanism of reliable sexual signaling. Sexual selection appears to be more intense in sexually dichromatic bird species, and comparative studies using pairwise comparisons of closely related taxa reveal that sexually dichromatic bird species have larger spleens, larger bursa of Fabricius, and higher concentrations of leukocytes than monochromatic species. Parasite-mediated sexual selection is proposed to affect parasite biology by increasing (1) the variance-to-mean ratio in parasite abundance, (2) variance in the intensity of natural selection affecting hosts, and (3) speciation rates among parasites exploiting hosts subject to intense sexual selection as compared to those subject to less intense selection. PMID:7799157

  19. Relic behaviours, coevolution and the retention versus loss of host defences after episodes of avian brood parasitism.

    PubMed

    Rothstein, Stephen I.

    2001-01-01

    Most previous studies of brood parasitism have stressed that host defences, such as egg recognition, are lost in the absence of parasitism. Such losses could result in coevolutionary cycles in which parasites shift away from well-defended hosts only to switch back to them later at a time when these hosts have lost much or all of their defences and the parasite's current hosts have built up effective defences. However, the alternative 'single trajectory' model predicts that parasites rarely switch back to old hosts because ex-hosts retain egg recognition for long periods in the absence of parasitism. If true, egg recognition by the host may be a 'relic behaviour', because in the absence of parasitism its adaptive value is close to neutral. Using artificial nonmimetic eggs, I tested for egg recognition in two populations that are currently unparasitized but that are descended from lineages likely to have been parasitized in the past: the grey catbird, Dumetella carolinensis, on Bermuda and the loggerhead shrike, Lanius ludovicianus, in California. Both of these populations showed long-term retention, ejecting nonmimetic eggs at rates of nearly 100%. Because potential present-day selection pressures, such as conspecific parasitism, do not explain this egg recognition, Bermuda catbirds apparently retain recognition from North American conspecifics that were cowbird hosts before colonizing Bermuda and shrikes retain recognition from Old World congeners that were hosts of cuckoos. Retention is also indicated by passerines in California and the Caribbean that had high rejection rates of nonmimetic eggs before coming into contact with cowbirds. These new data suggest that both the coevolutionary cycles and single trajectory models have importance and that rejection behaviour can have insignificant costs, which is consistent with evolutionary lag explanations for the acceptance of parasitic eggs shown by some cuckoo and many cowbird hosts. Copyright 2001 The Association for

  20. Endosymbiosis of Chlorella species to the ciliate Paramecium bursaria alters the distribution of the host's trichocysts beneath the host cell cortex.

    PubMed

    Kodama, Yuuki; Fujishima, Masahiro

    2011-04-01

    Each symbiotic Chlorella of the ciliate Paramecium bursaria is enclosed in a perialgal vacuole membrane derived from the host digestive vacuole membrane. Alga-free paramecia and symbiotic algae can grow independently. Mixing them experimentally can cause reinfection. Earlier, we reported that the symbiotic algae appear to push the host trichocysts aside to become fixed beneath the host cell cortex during the algal reinfection process. Indirect immunofluorescence microscopy with a monoclonal antibody against the trichocysts demonstrates that the trichocysts change their locality to form algal attachment sites and decrease their density beneath the host cell cortex through algal reinfection. Transmission electron microscopy to detect acid phosphatase activity showed that some trichocysts near the host cell cortex are digested by the host lysosomal fusion during algal reinfection. Removal of algae from the host cell using cycloheximide recovers the trichocyst's arrangement and number near the host cell cortex. These results indicate that symbiotic algae compete for their attachment sites with preexisting trichocysts and that the algae have the ability to ensure algal attachment sites beneath the host cell cortex.

  1. Exoplanets and their Host Stars

    NASA Astrophysics Data System (ADS)

    Schmitt, J.

    2016-06-01

    Among the most fundamental astrophysical discoveries are clearly the detections of many thousands of ``extrasolar'' planets orbiting their hosts. The majority of these new planetary systems have properties dramatically different from those in our solar system. The large distances to extrasolar planets imply that they can only be observed together with their hosts. Modern observations have shown that stars and planets are not merely accidental celestial neighbors bound by the force of gravity, rather they influence each other in a variety of ways. This also and specifically applies to the X-ray properties of exoplanet systems which I will review in my talk and give some ideas for future work in this area.

  2. Brood parasitism selects for no defence in a cuckoo host.

    PubMed

    Krüger, Oliver

    2011-09-22

    In coevolutionary arms races, like between cuckoos and their hosts, it is easy to understand why the host is under selection favouring anti-parasitism behaviour, such as egg rejection, which can lead to parasites evolving remarkable adaptations to 'trick' their host, such as mimetic eggs. But what about cases where the cuckoo egg is not mimetic and where the host does not act against it? Classically, such apparently non-adaptive behaviour is put down to evolutionary lag: given enough time, egg mimicry and parasite avoidance strategies will evolve. An alternative is that absence of egg mimicry and of anti-parasite behaviour is stable. Such stability is at first sight highly paradoxical. I show, using both field and experimental data to parametrize a simulation model, that the absence of defence behaviour by Cape bulbuls (Pycnonotus capensis) against parasitic eggs of the Jacobin cuckoo (Clamator jacobinus) is optimal behaviour. The cuckoo has evolved massive eggs (double the size of bulbul eggs) with thick shells, making it very hard or impossible for the host to eject the cuckoo egg. The host could still avoid brood parasitism by nest desertion. However, higher predation and parasitism risks later in the season makes desertion more costly than accepting the cuckoo egg, a strategy aided by the fact that many cuckoo eggs are incorrectly timed, so do not hatch in time and hence do not reduce host fitness to zero. Selection will therefore prevent the continuation of any coevolutionary arms race. Non-mimetic eggs and absence of defence strategies against cuckoo eggs will be the stable, if at first sight paradoxical, result.

  3. Pollination niche overlap between a parasitic plant and its host.

    PubMed

    Ollerton, Jeff; Stott, Adrian; Allnutt, Emma; Shove, Sam; Taylor, Chloe; Lamborn, Ellen

    2007-03-01

    Niche theory predicts that species which share resources should evolve strategies to minimise competition for those resources, or the less competitive species would be extirpated. Some plant species are constrained to co-occur, for example parasitic plants and their hosts, and may overlap in their pollination niche if they flower at the same time and attract the same pollinators. Using field observations and experiments between 1996 and 2006, we tested a series of hypotheses regarding pollination niche overlap between a specialist parasitic plant Orobanche elatior (Orobanchaceae) and its host Centaurea scabiosa (Asteraceae). These species flower more or less at the same time, with some year-to-year variation. The host is pollinated by a diverse range of insects, which vary in their effectiveness, whilst the parasite is pollinated by a single species of bumblebee, Bombus pascuorum, which is also an effective pollinator of the host plant. The two species therefore have partially overlapping pollination niches. These niches are not finely subdivided by differential pollen placement, or by diurnal segregation of the niches. We therefore found no evidence of character displacement within the pollination niches of these species, possibly because pollinators are not a limiting resource for these plants. Direct observation of pollinator movements, coupled with experimental manipulations of host plant inflorescence density, showed that Bombus pascuorum only rarely moves between inflorescences of the host and the parasite and therefore the presence of one plant is unlikely to be facilitating pollination in the other. This is the first detailed examination of pollination niche overlap in a plant parasite system and we suggest avenues for future research in relation to pollination and other shared interactions between parasitic plants and their hosts.

  4. Host factors exploited by retroviruses.

    PubMed

    Goff, Stephen P

    2007-04-01

    Retroviruses make a long and complex journey from outside the cell to the nucleus in the early stages of infection, and then an equally long journey back out again in the late stages of infection. Ongoing efforts are identifying an enormous array of cellular proteins that are used by the viruses in the course of their travels. These host factors are potential new targets for therapeutic intervention.

  5. Host specificity of parasite manipulation

    PubMed Central

    2012-01-01

    Recently we presented how Camponotus ants in Thailand infected with the fungus Ophiocordyceps unilateralis are behaviorally manipulated into dying where the conditions are optimal for fungal development. Death incurred in a very narrow zone of space and here we compare this highly specific manipulation with a related system in Brazil. We show that the behavioral manipulation is less fine-tuned and discuss the potential explanations for this by examining differences in ant host and environmental characteristics. PMID:22808322

  6. Host thin films incorporating nanoparticles

    NASA Astrophysics Data System (ADS)

    Qureshi, Uzma

    The focus of this research project was the investigation of the functional properties of thin films that incorporate a secondary nanoparticulate phase. In particular to assess if the secondary nanoparticulate material enhanced a functional property of the coating on glass. In order to achieve this, new thin film deposition methods were developed, namely use of nanopowder precursors, an aerosol assisted transport technique and an aerosol into atmospheric pressure chemical vapour deposition system. Aerosol assisted chemical vapour deposition (AACVD) was used to deposit 8 series of thin films on glass. Five different nanoparticles silver, gold, ceria, tungsten oxide and zinc oxide were tested and shown to successfully deposit thin films incorporating nanoparticles within a host matrix. Silver nanoparticles were synthesised and doped within a titania film by AACVD. This improved solar control properties. A unique aerosol assisted chemical vapour deposition (AACVD) into atmospheric pressure chemical vapour deposition (APCVD) system was used to deposit films of Au nanoparticles and thin films of gold nanoparticles incorporated within a host titania matrix. Incorporation of high refractive index contrast metal oxide particles within a host film altered the film colour. The key goal was to test the potential of nanopowder forms and transfer the suspended nanopowder via an aerosol to a substrate in order to deposit a thin film. Discrete tungsten oxide nanoparticles or ceria nanoparticles within a titanium dioxide thin film enhanced the self-cleaning and photo-induced super-hydrophilicity. The nanopowder precursor study was extended by deposition of zinc oxide thin films incorporating Au nanoparticles and also ZnO films deposited from a ZnO nanopowder precursor. Incorporation of Au nanoparticles within a VO: host matrix improved the thermochromic response, optical and colour properties. Composite VC/TiC and Au nanoparticle/V02/Ti02 thin films displayed three useful

  7. Pathogen host switching in commercial trade with management recommendations.

    PubMed

    Picco, Angela M; Karam, Abraham P; Collins, James P

    2010-06-01

    Global wildlife trade exacerbates the spread of nonindigenous species. Pathogens also move with hosts through trade and often are released into naïve populations with unpredictable outcomes. Amphibians are moved commercially for pets, food, bait, and biomedicine, and are an excellent model for studying how wildlife trade relates to pathogen pollution. Ranaviruses are amphibian pathogens associated with annual population die-offs; multiple strains of tiger salamander ranaviruses move through the bait trade in the western United States. Ranaviruses infect amphibians, reptiles, and fish and are of additional concern because they can switch hosts. Tiger salamanders are used as live bait for freshwater fishing and are a potential source for ranaviruses switching hosts from amphibians to fish. We experimentally injected largemouth bass with a bait trade tiger salamander ranavirus. Largemouth bass became infected but exhibited no signs of disease or mortality. Amphibian bait ranaviruses have the potential to switch hosts to infect fish, but fish may act as dead-end hosts or nonsymptomatic carriers, potentially spreading infection as a result of trade.

  8. Evolution of spatially structured host-parasite interactions.

    PubMed

    Lion, S; Gandon, S

    2015-01-01

    Spatial structure has dramatic effects on the demography and the evolution of species. A large variety of theoretical models have attempted to understand how local dispersal may shape the coevolution of interacting species such as host-parasite interactions. The lack of a unifying framework is a serious impediment for anyone willing to understand current theory. Here, we review previous theoretical studies in the light of a single epidemiological model that allows us to explore the effects of both host and parasite migration rates on the evolution and coevolution of various life-history traits. We discuss the impact of local dispersal on parasite virulence, various host defence strategies and local adaptation. Our analysis shows that evolutionary and coevolutionary outcomes crucially depend on the details of the host-parasite life cycle and on which life-history trait is involved in the interaction. We also discuss experimental studies that support the effects of spatial structure on the evolution of host-parasite interactions. This review highlights major similarities between some theoretical results, but it also reveals an important gap between evolutionary and coevolutionary models. We discuss possible ways to bridge this gap within a more unified framework that would reconcile spatial epidemiology, evolution and coevolution.

  9. Bats host major mammalian paramyxoviruses

    PubMed Central

    Drexler, Jan Felix; Corman, Victor Max; Müller, Marcel Alexander; Maganga, Gael Darren; Vallo, Peter; Binger, Tabea; Gloza-Rausch, Florian; Rasche, Andrea; Yordanov, Stoian; Seebens, Antje; Oppong, Samuel; Sarkodie, Yaw Adu; Pongombo, Célestin; Lukashev, Alexander N.; Schmidt-Chanasit, Jonas; Stöcker, Andreas; Carneiro, Aroldo José Borges; Erbar, Stephanie; Maisner, Andrea; Fronhoffs, Florian; Buettner, Reinhard; Kalko, Elisabeth K.V.; Kruppa, Thomas; Franke, Carlos Roberto; Kallies, René; Yandoko, Emmanuel R.N.; Herrler, Georg; Reusken, Chantal; Hassanin, Alexandre; Krüger, Detlev H.; Matthee, Sonja; Ulrich, Rainer G.; Leroy, Eric M.; Drosten, Christian

    2012-01-01

    The large virus family Paramyxoviridae includes some of the most significant human and livestock viruses, such as measles-, distemper-, mumps-, parainfluenza-, Newcastle disease-, respiratory syncytial virus and metapneumoviruses. Here we identify an estimated 66 new paramyxoviruses in a worldwide sample of 119 bat and rodent species (9,278 individuals). Major discoveries include evidence of an origin of Hendra- and Nipah virus in Africa, identification of a bat virus conspecific with the human mumps virus, detection of close relatives of respiratory syncytial virus, mouse pneumonia- and canine distemper virus in bats, as well as direct evidence of Sendai virus in rodents. Phylogenetic reconstruction of host associations suggests a predominance of host switches from bats to other mammals and birds. Hypothesis tests in a maximum likelihood framework permit the phylogenetic placement of bats as tentative hosts at ancestral nodes to both the major Paramyxoviridae subfamilies (Paramyxovirinae and Pneumovirinae). Future attempts to predict the emergence of novel paramyxoviruses in humans and livestock will have to rely fundamentally on these data. PMID:22531181

  10. Effects of host plant on life-history traits in the polyphagous spider mite Tetranychus urticae.

    PubMed

    Marinosci, Cassandra; Magalhães, Sara; Macke, Emilie; Navajas, Maria; Carbonell, David; Devaux, Céline; Olivieri, Isabelle

    2015-08-01

    Studying antagonistic coevolution between host plants and herbivores is particularly relevant for polyphagous species that can experience a great diversity of host plants with a large range of defenses. Here, we performed experimental evolution with the polyphagous spider mite Tetranychus urticae to detect how mites can exploit host plants. We thus compared on a same host the performance of replicated populations from an ancestral one reared for hundreds of generations on cucumber plants that were shifted to either tomato or cucumber plants. We controlled for maternal effects by rearing females from all replicated populations on either tomato or cucumber leaves, crossing this factor with the host plant in a factorial design. About 24 generations after the host shift and for all individual mites, we measured the following fitness components on tomato leaf fragments: survival at all stages, acceptance of the host plant by juvenile and adult mites, longevity, and female fecundity. The host plant on which mite populations had evolved did not affect the performance of the mites, but only affected their sex ratio. Females that lived on tomato plants for circa 24 generations produced a higher proportion of daughters than did females that lived on cucumber plants. In contrast, maternal effects influenced juvenile survival, acceptance of the host plant by adult mites and female fecundity. Independently of the host plant species on which their population had evolved, females reared on the tomato maternal environment produced offspring that survived better on tomato as juveniles, but accepted less this host plant as adults and had a lower fecundity than did females reared on the cucumber maternal environment. We also found that temporal blocks affected mite dispersal and both female longevity and fecundity. Taken together, our results show that the host plant species can affect critical parameters of population dynamics, and most importantly that maternal and environmental

  11. Effects of host plant on life-history traits in the polyphagous spider mite Tetranychus urticae.

    PubMed

    Marinosci, Cassandra; Magalhães, Sara; Macke, Emilie; Navajas, Maria; Carbonell, David; Devaux, Céline; Olivieri, Isabelle

    2015-08-01

    Studying antagonistic coevolution between host plants and herbivores is particularly relevant for polyphagous species that can experience a great diversity of host plants with a large range of defenses. Here, we performed experimental evolution with the polyphagous spider mite Tetranychus urticae to detect how mites can exploit host plants. We thus compared on a same host the performance of replicated populations from an ancestral one reared for hundreds of generations on cucumber plants that were shifted to either tomato or cucumber plants. We controlled for maternal effects by rearing females from all replicated populations on either tomato or cucumber leaves, crossing this factor with the host plant in a factorial design. About 24 generations after the host shift and for all individual mites, we measured the following fitness components on tomato leaf fragments: survival at all stages, acceptance of the host plant by juvenile and adult mites, longevity, and female fecundity. The host plant on which mite populations had evolved did not affect the performance of the mites, but only affected their sex ratio. Females that lived on tomato plants for circa 24 generations produced a higher proportion of daughters than did females that lived on cucumber plants. In contrast, maternal effects influenced juvenile survival, acceptance of the host plant by adult mites and female fecundity. Independently of the host plant species on which their population had evolved, females reared on the tomato maternal environment produced offspring that survived better on tomato as juveniles, but accepted less this host plant as adults and had a lower fecundity than did females reared on the cucumber maternal environment. We also found that temporal blocks affected mite dispersal and both female longevity and fecundity. Taken together, our results show that the host plant species can affect critical parameters of population dynamics, and most importantly that maternal and environmental

  12. Effects of host plant on life-history traits in the polyphagous spider mite Tetranychus urticae

    PubMed Central

    Marinosci, Cassandra; Magalhães, Sara; Macke, Emilie; Navajas, Maria; Carbonell, David; Devaux, Céline; Olivieri, Isabelle

    2015-01-01

    Studying antagonistic coevolution between host plants and herbivores is particularly relevant for polyphagous species that can experience a great diversity of host plants with a large range of defenses. Here, we performed experimental evolution with the polyphagous spider mite Tetranychus urticae to detect how mites can exploit host plants. We thus compared on a same host the performance of replicated populations from an ancestral one reared for hundreds of generations on cucumber plants that were shifted to either tomato or cucumber plants. We controlled for maternal effects by rearing females from all replicated populations on either tomato or cucumber leaves, crossing this factor with the host plant in a factorial design. About 24 generations after the host shift and for all individual mites, we measured the following fitness components on tomato leaf fragments: survival at all stages, acceptance of the host plant by juvenile and adult mites, longevity, and female fecundity. The host plant on which mite populations had evolved did not affect the performance of the mites, but only affected their sex ratio. Females that lived on tomato plants for circa 24 generations produced a higher proportion of daughters than did females that lived on cucumber plants. In contrast, maternal effects influenced juvenile survival, acceptance of the host plant by adult mites and female fecundity. Independently of the host plant species on which their population had evolved, females reared on the tomato maternal environment produced offspring that survived better on tomato as juveniles, but accepted less this host plant as adults and had a lower fecundity than did females reared on the cucumber maternal environment. We also found that temporal blocks affected mite dispersal and both female longevity and fecundity. Taken together, our results show that the host plant species can affect critical parameters of population dynamics, and most importantly that maternal and environmental

  13. Survival relative to new and ancestral host plants, phytoplasma infection, and genetic constitution in host races of a polyphagous insect disease vector.

    PubMed

    Maixner, Michael; Albert, Andreas; Johannesen, Jes

    2014-08-01

    Dissemination of vectorborne diseases depends strongly on the vector's host range and the pathogen's reservoir range. Because vectors interact with pathogens, the direction and strength of a vector's host shift is vital for understanding epidemiology and is embedded in the framework of ecological specialization. This study investigates survival in host-race evolution of a polyphagous insect disease vector, Hyalesthes obsoletus, whether survival is related to the direction of the host shift (from field bindweed to stinging nettle), the interaction with plant-specific strains of obligate vectored pathogens/symbionts (stolbur phytoplasma), and whether survival is related to genetic differentiation between the host races. We used a twice repeated, identical nested experimental design to study survival of the vector on alternative hosts and relative to infection status. Survival was tested with Kaplan-Meier analyses, while genetic differentiation between vector populations was quantified with microsatellite allele frequencies. We found significant direct effects of host plant (reduced survival on wrong hosts) and sex (males survive longer than females) in both host races and relative effects of host (nettle animals more affected than bindweed animals) and sex (males more affected than females). Survival of bindweed animals was significantly higher on symptomatic than nonsymptomatic field bindweed, but in the second experiment only. Infection potentially had a positive effect on survival in nettle animals but due to low infection rates the results remain suggestive. Genetic differentiation was not related to survival. Greater negative plant-transfer effect but no negative effect of stolbur in the derived host race suggests preadaptation to the new pathogen/symbiont strain before strong diversifying selection during the specialization process. Physiological maladaptation or failure to accept the ancestral plant will have similar consequences, namely positive assortative

  14. Evaluating the within-host fitness effects of mutations fixed during virus adaptation to different ecotypes of a new host

    PubMed Central

    Hillung, Julia; Cuevas, José M.; Elena, Santiago F.

    2015-01-01

    The existence of genetic variation for resistance in host populations is assumed to be essential to the spread of an emerging virus. Models predict that the rate of spread slows down with the increasing frequency and higher diversity of resistance alleles in the host population. We have been using the experimental pathosystem Arabidopsis thaliana—tobacco etch potyvirus (TEV) to explore the interplay between genetic variation in host's susceptibility and virus diversity. We have recently shown that TEV populations evolving in A. thaliana ecotypes that differ in susceptibility to infection gained within-host fitness, virulence and infectivity in a manner compatible with a gene-for-gene model of host–parasite interactions: hard-to-infect ecotypes were infected by generalist viruses, whereas easy-to-infect ecotypes were infected by every virus. We characterized the genomes of the evolved viruses and found cases of host-driven convergent mutations. To gain further insights in the mechanistic basis of this gene-for-gene model, we have generated all viral mutations individually as well as in specific combinations and tested their within-host fitness effects across ecotypes. Most of these mutations were deleterious or neutral in their local ecotype and only a very reduced number had a host-specific beneficial effect. We conclude that most of the mutations fixed during the evolution experiment were so by drift or by selective sweeps along with the selected driver mutation. In addition, we evaluated the ruggedness of the underlying adaptive fitness landscape and found that mutational effects were mostly multiplicative, with few cases of significant epistasis. PMID:26150658

  15. Parasite transmission in complex communities: predators and alternative hosts alter pathogenic infections in amphibians.

    PubMed

    Orlofske, Sarah A; Jadin, Robert C; Preston, Daniel L; Johnson, Pieter T J

    2012-06-01

    While often studied in isolation, host-parasite interactions are typically embedded within complex communities. Other community members, including predators and alternative hosts, can therefore alter parasite transmission (e.g., the dilution effect), yet few studies have experimentally evaluated more than one such mechanism. Here, we used data from natural wetlands to design experiments investigating how alternative hosts and predators of parasites mediate trematode (Ribeiroia ondatrae) infection in a focal amphibian host (Pseudacris regilla). In short-term predation bioassays involving mollusks, zooplankton, fish, larval insects, or newts, four of seven tested species removed 62-93% of infectious stages. In transmission experiments, damselfly nymphs (predators) and newt larvae (alternative hosts) reduced infection in P. regilla tadpoles by -50%, whereas mosquitofish (potential predators and alternative hosts) did not significantly influence transmission. Additional bioassays indicated that predators consumed parasites even in the presence of alternative prey. In natural wetlands, newts had similar infection intensities as P. regilla, suggesting that they commonly function as alternative hosts despite their unpalatability to downstream hosts, whereas mosquitofish had substantially lower infection intensities and are unlikely to function as hosts. These results underscore the importance of studying host-parasite interactions in complex communities and of broadly linking research on predation, biodiversity loss, and infectious diseases.

  16. Infection success of different trematode genotypes in two alternative intermediate hosts: evidence for intraspecific specialization?

    PubMed

    Leung, T L F; Poulin, R

    2010-02-01

    The evolution of host specificity and the potential trade-off between being a generalist and a specialist are central issues in the evolutionary ecology of parasites. Different species of parasites or even different populations of the same species often show different degrees of host specificity. However, less is known about intraspecific variation in host specificity within a population. We investigated intraspecific variation by experimentally exposing cercariae from different clones of the trematode Curtuteria australis to two species of second intermediate hosts, the New Zealand cockle Austrovenus stutchburyi and the wedge shell Macomona liliana. We found an overall difference in infection success between the two bivalve species, with A. stutchburyi being the more heavily infected host. However, the cercariae showed a consistent preference for encysting at the tip of the bivalve's foot, regardless of host species. Importantly, there were no significant differences among parasite clones in either relative infection success in the two hosts or preference for the host foot tip. This lack of intraspecific variation may be due to the life-history traits of both parasite and hosts in our system, which may limit opportunities for variation in performance and exploitation strategies in different hosts to evolve within the population.

  17. Impact of road traffic emissions on tropospheric ozone in Europe for present day and future scenarios

    NASA Astrophysics Data System (ADS)

    Mertens, Mariano; Kerkweg, Astrid; Grewe, Volker; Jöckel, Patrick

    2016-04-01

    Road traffic is an important anthropogenic source of NOx, CO and non-methane hydrocarbons (NMHCs) which act as precursors for the formation of tropospheric ozone. The formation of ozone is highly non-linear. This means that the contribution of the road traffic sector cannot directly be derived from the amount of emitted species, because they are also determined by local emissions of other anthropogenic and natural sources. In addition, long range transport of precursors and ozone can play an important role in determining the local ozone budget. For a complete assessment of the impact of road traffic emissions it is therefore important to resolve both, local emissions and long range transport. This can be achieved by the use of the newly developed MECO(n) model system, which on-line couples the global chemistry-climate-model EMAC with the regional chemistry-climate-model COSMO-CLM/MESSy. Both models use the same chemical speciation. This allows a highly consistent model chain from the global to the local scale. To quantify the contribution of the road traffic emissions to tropospheric ozone we use an accounting system of the relevant reaction pathways of the different species from different sources (called tagging method). This tagging scheme is implemented consistently on all scales, allowing a direct comparison of the contributions. With this model configuration we investigate the impact of road traffic emissions to the tropospheric ozone budget in Europe. For the year 2008 we compare different emission scenarios and investigate the influence of both model and emission resolution. In addition, results of a mitigation scenario for the year 2030 are presented. They indicate that the contribution of the road traffic sector can be reduced by local reductions of emissions during summer. During winter the importance of long range transport increases. This can lead to increased contributions of the road traffic sector (e.g. by increased emissions in the US) even if local emissions are reduced.

  18. Present-day star formation: From molecular cloud cores to protostars and protoplanetary disks

    NASA Astrophysics Data System (ADS)

    Inutsuka, Shu-ichiro

    2012-10-01

    Essential physical processes in the formation of protostars and protoplanetary disks are described. Recent advances in non-ideal magnetohydrodynamics simulations, which cover a huge dynamic range from molecular cloud core density (104/cc) to stellar density (1022/cc) in a self-consistent manner, enable us to study the realistic evolution of the magnetic field and rotation of protostars and the dynamics of outflows and jets. First we emphasize the importance of radiative heating and cooling, and describe thermal evolution in a self-gravitationally collapsing cloud. The increased pressure at the center creates the first hydrostatic core, which consists of molecular gas. After the dissociation of molecular hydrogen triggers the second gravitational collapse at the center of the first core, a protostar is quickly formed and the first core gradually transforms into a circumstellar disk that eventually accretes onto the central protostar. The importance of the short-lived first core formed in the early collapsing phase is emphasized in the contexts of driving magnetohydrodynamical bipolar outflows and self-gravitational fragmentation into binary or multiple stars. When the central density becomes sufficiently high (1012/cc), ohmic dissipation largely removes the magnetic flux from a collapsing cloud core, and the strongly twisted magnetic field lines are straightened. The magnetic field lines are twisted and amplified again for much higher density (1016/cc) where the magnetic field is recoupled with warm gas (˜103 K). Finally, protostars at their formation epoch have magnetic fields of 0.1-1 kG, which is comparable to observed values of pre-main-sequence stars. A substantially reduced magnetic flux at the center results in passively wound-up magnetic field lines just after the formation of a protostar. Th