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Sample records for howard pat dale

  1. Dale Sayers Festschrift

    SciTech Connect

    Stern, Edward A.

    2007-02-02

    This Festschrift is in honor of Dale Sayers who passed away in November 2004. Dale played a pivotal role in initiating the modern era of X-ray Absorption Fine Structure (XAFS) 35 years ago. The prehistory of XAFS before the modern era consisted of 40 years of confusion caused by Kronig's two different theories of the extended XAFS (EXAFS), the Short-Range Order (SRO) and Long-Range Order (LRO) theories. Dale's PhD thesis on EXAFS led to the idea of a Fourier transform to definitely prove that SRO is the correct theory and then to the development of XAFS as a structure determination technique.

  2. Pat Mora.

    ERIC Educational Resources Information Center

    Corsaro, Julie

    1997-01-01

    Discusses Pat Mora's career as a children's book author and aspects of her work: the Latino experience, bilingualism, family life, desert landscape, and folk tales. Provides classroom activities in art, creative writing, social studies, geography, drama. Provides a selected bibliography of Mora's work. (PEN)

  3. Dale Gladden: An Omega Interview.

    ERIC Educational Resources Information Center

    Kastenbaum, Robert

    1994-01-01

    Presents interview with Dale Gladden, who has worked as emergency medical technician, paramedic, and emergency room nurse. Focuses on Gladden's experiences as paramedic and looks ahead to further studies he has since pursued. Gladden explains how he chose to become paramedic, describes what characteristics make a good paramedic, and discusses…

  4. Reinventing Howard's Law School.

    ERIC Educational Resources Information Center

    Glenn, Gwendolyn

    2001-01-01

    Describes efforts by Alice Gresham Bullock, dean of the Howard University School of Law, to improve the school (including building a new library and improving relations with faculty) and restore Howard to its previous stature as a leader in civil rights law. (EV)

  5. John Galen Howard.

    ERIC Educational Resources Information Center

    Draper, Joan

    1979-01-01

    A biographical sketch of John Galen Howard, founder of the Department of Architecture at the University of California at Berkeley, is presented. Howard's conservative outlook and idealistic nature are examined and his influence on the curriculum at the university is traced. (PHR)

  6. Howard University Bookstore

    ERIC Educational Resources Information Center

    Maxon, Hazel Carter; Negron, Jaime

    1977-01-01

    Two full-time university bookstores, with three satellites helping during rush period, serve the Howard students and faculty. Solutions to problems of space, acquiring used books, and communications with faculty members are discussed, and the successful retailing of black studies books is described. (LBH)

  7. The Howard Zinn Show

    ERIC Educational Resources Information Center

    Sewall, Gilbert T.

    2012-01-01

    Howard Zinn's "A People's History of the United States" is the nation's best-known work of American history. It is also the nation's best-selling survey of American history, having sold two million copies since its publication in 1980 and still selling about 125,000 paperback copies yearly. The fifth and current edition covers America up to the…

  8. Howard Zinn on Democratic Education

    ERIC Educational Resources Information Center

    Zinn, Howard

    2004-01-01

    Perhaps no other historian has had a more profound and revolutionary impact on American education than Howard Zinn. This is the first book devoted to his views on education and its role in a democratic society. "Howard Zinn on Democratic Education" describes what is missing from school textbooks and in classrooms--and how we move beyond these…

  9. Implementing PAT with Standards

    NASA Astrophysics Data System (ADS)

    Chandramohan, Laakshmana Sabari; Doolla, Suryanarayana; Khaparde, S. A.

    2016-02-01

    Perform Achieve Trade (PAT) is a market-based incentive mechanism to promote energy efficiency. The purpose of this work is to address the challenges inherent to inconsistent representation of business processes, and interoperability issues in PAT like cap-and-trade mechanisms especially when scaled. Studies by various agencies have highlighted that as the mechanism evolves including more industrial sectors and industries in its ambit, implementation will become more challenging. This paper analyses the major needs of PAT (namely tracking, monitoring, auditing & verifying energy-saving reports, and providing technical support & guidance to stakeholders); and how the aforesaid reasons affect them. Though current technologies can handle these challenges to an extent, standardization activities for implementation have been scanty for PAT and this work attempts to evolve them. The inconsistent modification of business processes, rules, and procedures across stakeholders, and interoperability among heterogeneous systems are addressed. This paper proposes the adoption of specifically two standards into PAT, namely Business Process Model and Notation for maintaining consistency in business process modelling, and Common Information Model (IEC 61970, 61968, 62325 combined) for information exchange. Detailed architecture and organization of these adoptions are reported. The work can be used by PAT implementing agencies, stakeholders, and standardization bodies.

  10. Howard University Assembles Fund-Raising Juggernaut

    ERIC Educational Resources Information Center

    Masterson, Kathryn

    2008-01-01

    As a dental student 35 years ago, Leo E. Rouse and his Howard University classmates learned to fill cavities and cap teeth by crowding around one faculty member and angling for a clear view of the day's demonstration. Today students at Howard's College of Dentistry, where Dr. Rouse is now the dean, get an unobstructed view of dental procedures…

  11. Howard Zinn and the Socially Conscious Academic

    ERIC Educational Resources Information Center

    McKivigan, John R.

    2009-01-01

    In recent decades many people came to know Howard Zinn for his outspoken advocacy on a wide range of progressive causes, including civil rights, free speech, workers' rights, education reform, and opposition to U.S. imperialism. The author's own first encounter with Howard Zinn's special combination of scholarship and activism occurred several…

  12. Oral Histories in Meteoritics and Planetary Science - XX: Dale Cruikshank

    NASA Astrophysics Data System (ADS)

    Sears, Derek W. G.

    2013-04-01

    In this interview, Dale Cruikshank (Fig. 1) explains how as an undergraduate at Iowa State University he was a summer student at Yerkes Observatory where he assisted Gerard Kuiper in work on his Photographic Lunar Atlas. Upon completing his degree, Dale went to graduate school at the University of Arizona with Kuiper where he worked on the IR spectroscopy of the lunar surface. After an eventful 1968 trip to Moscow via Prague, during which the Soviets invaded Czechoslovakia, Dale assumed a postdoc position with Vasili Moroz at the Sternberg Astronomical Institute and more observational IR astronomy. Upon returning to the United States and after a year at Arizona, Dale assumed a position at the University of Hawai'i that he held for 17 years. During this period Dale worked with others on thermal infrared determinations of the albedos of small bodies beyond the asteroid Main Belt, leading to the recognition that low-albedo material is prevalent in the outer solar system that made the first report of complex organic solids on a planetary body (Saturn's satellite Iapetus). After moving to Ames Research Center, where he works currently, he continued this work and became involved in many outer solar system missions. Dale has served the community through his involvement in developing national policies for science-driven planetary exploration, being chair of the DPS 1990-1991 and secretary/treasurer for 1982-1985. He served as president of Commission 16 (Physics of Planets) of the IAU (2001-2003). He received the Kuiper prize in 2006.

  13. Dale Chihuly: An Inspiration in Art, Science, and Math!

    ERIC Educational Resources Information Center

    Hubbert, Beth

    2009-01-01

    Connecting students to the arts in a concrete way can be an effective teaching tool. In this article, the author describes how Dale Chihuly's "Hart Window," which features hand-blown glass disks affixed to the framework of the window, can be an inspiration for interdisciplinary connections in art, science and math. (Contains 4 online resources.)

  14. Aesthetics at the Impasse: The Unresolved Property of Dale Farm

    ERIC Educational Resources Information Center

    McCarthy, Lynne

    2015-01-01

    "Soil Depositions" was an art activist project that responded to the 2011 Irish Traveller eviction at Dale Farm in Essex when three resident women donated small amounts of soil from the site of their former home. The soil was subsequently deposited, framed and documented in various national and international locations. This article…

  15. Astronaut Dale Gardner holds up for sale sign after EVA

    NASA Technical Reports Server (NTRS)

    1984-01-01

    Astronaut Dale A. Gardner, having just completed the major portion of his second extravehicular activity (EVA) period in three days, holds up a 'for sale' sign. Astronaut Joseph P. ALlen IV, who also participated in the two EVA's, is reflected in Gardner's helmet visor. A portion of each of two recovered satellites is in the lower right corner, with Westar nearer Discovery's aft.

  16. Howard Parnes, MD | Division of Cancer Prevention

    Cancer.gov

    Dr. Howard Parnes received a BA from Cornell University in 1977 and an MD from the University of Medicine and Dentistry of New Jersey in 1981. He trained in internal medicine at the Johns Hopkins Bayview Medical Center from 1981 to 1984 followed by a medical oncology fellowship at the University of Maryland Cancer Center (UMCC) from 1984 to 1987. |

  17. Howard Community College 1998 Performance Accountability Report.

    ERIC Educational Resources Information Center

    Howard Community Coll., Columbia, MD. Office of Planning and Evaluation.

    This is a report on the 1998 performance accountability assessment conducted by Howard Community College (HCC) (Maryland) to examine overall institutional effectiveness. Enrollment trends show that more students are attending HCC, which will require more facility space, instructors, and student support. HCC addresses this issue by offering more…

  18. Howard Hughes and His Colorful Aircraft Career

    ERIC Educational Resources Information Center

    Karwatka, Dennis

    2012-01-01

    The HK-1 "Hercules" airplane made its maiden flight over 60 years ago, and it still holds the record as the airplane with the largest wingspan that ever flew. Powered by eight massive 28-cylinder engines, it was piloted by Howard Hughes during its one brief flight in California. A large portion of the airplane was made of wood, which gave rise to…

  19. The scientific legacy of Howard Vincent Malmstadt

    NASA Astrophysics Data System (ADS)

    Horlick, Gary

    2006-06-01

    Howard Malmstadt was a true giant of Analytical Chemistry and clearly one of the most influential analytical chemists of the last 50 years. Howard, through his own work and that of his students (first generation) and their students (second generation) and their students' students (third generation) changed the course of Analytical Chemistry. His research interests were broad and ranged from analytical solution chemistry (titrimetry and reaction rates) and electrochemistry to atomic and molecular spectroscopy, chemical instrumentation, clinical chemistry and automation. Howard was also one of the most innovative and influential educators of our time. He changed forever the analytical curriculum through his many books on Electronics for Scientists, most written in conjunction with Chris Enke and Stan Crouch. Their texts and short courses went from pioneering the application of tube-based analog electronics (servo systems and operational amplifiers) in scientific measurements to the impact that integrated circuits and digital electronics would have on laboratory measurements. He strongly believed in the importance of "hands-on" in education. To this end, he expended considerable personal effort and time to see not only the development and commercialization of an effective laboratory infrastructure to support education in analog and digital electronics, but also oversaw the development of modular instrumentation for spectroscopy. Over the years he received many awards from the Analytical Chemistry community for his outstanding efforts and contributions to teaching and research. Many of Howard's students went on into academia. They and their students now represent the ongoing legacy for analytical chemistry that evolved from Howard's laboratory at Illinois. A remarkable diversity of research programs are underway in their laboratories. Topics range from atomic, laser, mass, and Raman spectroscopy to detection technology, analytical education, micro

  20. Functional dissection and evidence for intercellular transfer of the heterocyst-differentiation PatS morphogen.

    PubMed

    Corrales-Guerrero, Laura; Mariscal, Vicente; Flores, Enrique; Herrero, Antonia

    2013-06-01

    The formation of a diazotrophic cyanobacterial filament represents a simple example of biological development. In Anabaena, a non-random pattern of one nitrogen-fixing heterocyst separated by about 10 photosynthetic vegetative cells results from lateral inhibition elicited by the cells differentiating into heterocysts. Key to this process is the patS gene, which has been shown to produce an inhibitor of heterocyst differentiation that involves the C-terminal RGSGR pentapeptide. Complementation of a ΔpatS Anabaena mutant with different versions of PatS, including point mutations or tag fusions, showed that patS is translated into a 17-amino acid polypeptide. Alterations in the N-terminal part of PatS produced inhibition of heterocyst differentiation, thus this part of the peptide appears necessary for proper processing and self-immunity in the producing cells. Alterations in the C-terminal part of PatS led to over-differentiation, thus supporting its role in inhibition of heterocyst differentiation. A polypeptide, produced in proheterocysts, consisting of a methionine followed by the eight, but not the five, terminal amino acids of PatS recreated the full activity of the native peptide. Immunofluorescence detection showed that an RGSGR-containing peptide accumulated in the cells adjacent to the producing proheterocysts, illustrating intercellular transfer of a morphogen in the cyanobacterial filaments. PMID:23663167

  1. Howard University Engineers Success: Interdisciplinary Study Keeps Howard on the Cutting Edge

    ERIC Educational Resources Information Center

    Chew, Cassie M.

    2004-01-01

    According to Engineering Workforce Commission annual reports, in 1999 Howard University graduated 108 students, 92 of whom were African American, in its chemical, civil, electrical, and mechanical engineering programs and computer science programs. After two more years of graduating approximately 100 students across programs, in 2002, according to…

  2. Howard H. Kendler (1919-2011).

    PubMed

    Foley, John M

    2012-09-01

    Presents an obituary for Howard H. Kendler. Kendler was born in New York City on June 9, 1919, and died in Santa Barbara, California, on February 17, 2011. Kendler majored in psychology at Brooklyn College, where he was an assistant to Abraham Maslow, the father of humanistic psychology, and did a research project on the psychology of thinking under the supervision of Solomon Asch, a leading Gestalt psychologist. Howard Kendler's career was characterized by insightful experiments and a consistent analysis of the fundamental issues of the nature of our science, its methods, and its role in the community. The breadth of his knowledge and the depth of his thinking on these issues are rare. Although he did not settle these issues, he brought them into clear focus and forcefully advocated for his point of view. He was a visiting professor at the University of California, Berkeley, Hebrew University in Jerusalem, and Tel-Aviv University and was a fellow at the Center for Advanced Study in the Behavioral Sciences. He held the offices of president of the Western Psychological Association, chairman of the Board of Governors of the Psychonomic Society, and president of the Division of General Psychology and the Division of Experimental Psychology of the American Psychological Association.

  3. Howard H. Kendler (1919-2011).

    PubMed

    Foley, John M

    2012-09-01

    Presents an obituary for Howard H. Kendler. Kendler was born in New York City on June 9, 1919, and died in Santa Barbara, California, on February 17, 2011. Kendler majored in psychology at Brooklyn College, where he was an assistant to Abraham Maslow, the father of humanistic psychology, and did a research project on the psychology of thinking under the supervision of Solomon Asch, a leading Gestalt psychologist. Howard Kendler's career was characterized by insightful experiments and a consistent analysis of the fundamental issues of the nature of our science, its methods, and its role in the community. The breadth of his knowledge and the depth of his thinking on these issues are rare. Although he did not settle these issues, he brought them into clear focus and forcefully advocated for his point of view. He was a visiting professor at the University of California, Berkeley, Hebrew University in Jerusalem, and Tel-Aviv University and was a fellow at the Center for Advanced Study in the Behavioral Sciences. He held the offices of president of the Western Psychological Association, chairman of the Board of Governors of the Psychonomic Society, and president of the Division of General Psychology and the Division of Experimental Psychology of the American Psychological Association. PMID:22963414

  4. Howard Beach Youth: A Study of Racial and Ethnic Attitudes.

    ERIC Educational Resources Information Center

    Lichter, Linda S.; Lichter, S. Robert

    This assessment of the climate of racial and ethnic attitudes in Howard Beach (New York) was conducted at John Adams High School, the public school attended by the greatest number of high school children in the Howard Beach community. The survey of 1,217 students was administered in December, 1986, several weeks before the incident in which a…

  5. Report of Survey of Howard University College of Medicine.

    ERIC Educational Resources Information Center

    Office of Education (DHEW), Washington, DC.

    Howard University's School of Medicine is evaluated in this report, in fulfillment of the United States Congress's requirement that the Office of Education annually evaluate one aspect of the Howard University educational program. Evaluation teams, comprised mostly of Office of Education professionals, spent several days at the University meeting…

  6. Analysis of deaths caused by pat-pat accidents in Turkey.

    PubMed

    Küçüker, Hüdaverdi

    2012-01-01

    This study deals with deaths during the period 2004 to 2009 that occurred during the use of a vehicle known as pat-pat, which is an unusual vehicle, similar to a tractor-trailer used in agricultural production and transportation The records of crime scene investigation and autopsies of 28 cases of death due to pat-pat-related crushes were evaluated. Of all cases, 60.7 percent were drivers and the remaining 39.3% were passengers. The most common cause of death (in 39.3% of cases) was brain-spinal cord injury due to blunt trauma of the head and neck. Implementing legislation regarding safety measures or limitation of the use for transportation should be performed for pat-pats, of which thousands are currently in use.

  7. 18. Historic American Buildings Survey Photocopy of C. Howard Crane ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    18. Historic American Buildings Survey Photocopy of C. Howard Crane Office Drawing, 1919. Courtesy Smith, Hinchman & Grylls Associates, Inc., Detroit, 1973 UPPER MEZZANINE FLOOR PLAN - Orchestra Hall, 3711 Woodward Avenue, Detroit, MI

  8. 17. Historic American Buildings Survey Photocopy of C. Howard Crane ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    17. Historic American Buildings Survey Photocopy of C. Howard Crane Office Drawing, 1919. Courtesy Smith, Hinchman & Grylls Associates, Inc., Detroit, 1973 MEZZANINE FLOOR PLAN - Orchestra Hall, 3711 Woodward Avenue, Detroit, MI

  9. 14. Historic American Buildings Survey Photocopy of C. Howard Crane ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    14. Historic American Buildings Survey Photocopy of C. Howard Crane Office Drawing, 1919, Courtesy Smith, Hinchman & Grylls Associates, Inc., Detroit, 1973 PLOT PLAN (TITLE SHEET) - Orchestra Hall, 3711 Woodward Avenue, Detroit, MI

  10. 12. Historic American Buildings Survey Rendering by C. Howard Crane ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    12. Historic American Buildings Survey Rendering by C. Howard Crane Office, 1919. Courtesy Detroit Historical Society. Photocopy by Jack E. Boucher, 1973 PERSPECTIVE VIEW FROM SOUTH - Orchestra Hall, 3711 Woodward Avenue, Detroit, MI

  11. 16. Historic American Buildings Survey Photocopy, C. Howard Crane Office ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    16. Historic American Buildings Survey Photocopy, C. Howard Crane Office Drawing of 1919. Courtesy Smith, Hinchman & Grylls Associates, Inc., Detroit, 1973 FIRST FLOOR PLAN - Orchestra Hall, 3711 Woodward Avenue, Detroit, MI

  12. 15. Historic American Buildings Survey Photocopy, C. Howard Crane Office ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    15. Historic American Buildings Survey Photocopy, C. Howard Crane Office Drawing of 1919. Courtesy Smith, Hinchman & Grylls Inc., Detroit, 1973 BASEMENT PLAN - Orchestra Hall, 3711 Woodward Avenue, Detroit, MI

  13. John Howard, England's Great Prison Reformer: His Glimpse into Hell.

    ERIC Educational Resources Information Center

    Roberts, Leonard H.

    1985-01-01

    Describes the life and work of John Howard, the great English prison reformer, who pioneered in exposing certain inequities of the English social system through his publications, his investigation of prison conditions first hand, and his appearances before Parliament. (CT)

  14. 32. Historic American Buildings Survey Photocopy of C. Howard Crane ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    32. Historic American Buildings Survey Photocopy of C. Howard Crane Office Drawing of 1919. Courtesy Smith, Hinchman & Grylls Associates, Inc., Detroit, 1973 ORNAMENTAL IRON DETAILS: FIRE ESCAPES, MAIN ENTRANCE DOORS, ETC. - Orchestra Hall, 3711 Woodward Avenue, Detroit, MI

  15. Time-dependent Brittle Deformation in Darley Dale Sandstone

    NASA Astrophysics Data System (ADS)

    Baud, P.; Heap, M. J.; Meredith, P. G.; Bell, A. F.; Main, I. G.

    2008-12-01

    The characterization of time-dependent brittle rock deformation is fundamental to understanding the long- term evolution and dynamics of the Earth's upper crust. The chemical influence of water promotes time- dependent deformation through stress corrosion cracking that allows rocks to deform at stresses far below their short-term failure strength. Here we report results from a study of time-dependent brittle creep in water- saturated samples of Darley Dale sandstone (initial porosity of 13%). Conventional creep experiments (or 'static fatigue' tests) show that time to failure decreases dramatically with the imposed deviatoric stress. They also suggest the existence of a critical level of damage beyond which localized failure develops. Sample variability results however in significant scattering in the experimental data and numerous tests are needed to clearly define a relation between the strain rate and the applied stress. We show here that stress-stepping experiments provide a means to overcome this problem and that it is possible this way to obtain the strain rate dependence on applied stress with a single test. This allows to study in details the impact of various thermodynamical conditions on brittle creep. The influence of effective stress was investigated in stress-stepping experiments with effective confining pressures of 10, 30 and 50 MPa (whilst maintaining a constant pore fluid pressure of 20 MPa). In addition to the expected purely mechanical influence of an elevated effective stress our results also demonstrate that stress corrosion appears to be inhibited at higher effective stresses. The influence of doubling the pore fluid pressure however, whilst maintaining a constant effective stress, is shown to have no effect on the rate of stress corrosion. We then discuss the results in light of acoustic emission hypocentre location data and optical microscope analysis and use our experimental data to validate proposed macroscopic creep laws. Finally, using

  16. Obituary: Howard H. Lanning, 1946-2007

    NASA Astrophysics Data System (ADS)

    Wade, Richard A.; MacConnell, D. Jack

    2009-01-01

    Howard H. Lanning died 20 December 2007 in Tucson, Arizona. He was a Software Quality Assurance Engineer for the Data Products Program at the National Optical Astronomy Observatory [NOAO] in Tucson, having returned to his native West after twenty years at the Space Telescope Science Institute [STScI] in Baltimore, Maryland. Throughout his career in roles supporting other astronomers, Howard steadily and persistently worked at his own research programs, most notably the identification and study of UV-bright sources in the Sandage Two-Color Survey of the Galactic Plane. Minor planet 2000 QJ248 has been named (61913) Lanning in recognition of his contributions. The son of James Clyde Lanning and Ethel Malan Lanning, Howard was born 26 May 1946, in Brawley, a small community near Calipatria, California. His parents worked for the local school district where his late father was Superintendent of Maintenance and Grounds and his mother was a school principal. After graduating from high school in a class of 52, Howard earned his A.A. degree at Imperial Valley Junior College, majoring in Astronomy and minoring in Mathematics. He completed his A.B. degree in Applied Arts and Sciences at San Diego State University [SDSU] in 1969 with the same major and minor. In 1974, he completed his M.S. in Astronomy at SDSU with a thesis on the period variation of the white-dwarf eclipsing binary BD +16∘516. During his time at SDSU Lanning was a Research Assistant/Observer at the former Hale Observatories, working on the optical identification of X-ray sources under the direction of Allan Sandage. His second refereed paper, the first of several finding lists of UV-bright stars arising from this work, appeared in 1973. Fellow students and faculty remember that he was a self-starter, initiating many observing projects of his own at Mount Laguna Observatory and encouraging others to do the same. For more than a decade Lanning was an observer and night assistant at Mount Wilson Observatory

  17. Howard Brenner's Legacy for Biological Transport Processes

    NASA Astrophysics Data System (ADS)

    Nitsche, Johannes

    2014-11-01

    This talk discusses the manner in which Howard Brenner's theoretical contributions have had, and long will have, strong and direct impact on the understanding of transport processes occurring in biological systems. His early work on low Reynolds number resistance/mobility coefficients of arbitrarily shaped particles, and particles near walls and in pores, is an essential component of models of hindered diffusion through many types of membranes and tissues, and convective transport in microfluidic diagnostic systems. His seminal contributions to macrotransport (coarse-graining, homogenization) theory presaged the growing discipline of multiscale modeling. For biological systems they represent the key to infusing diffusion models of a wide variety of tissues with a sound basis in their microscopic structure and properties, often over a hierarchy of scales. Both scientific currents are illustrated within the concrete context of diffusion models of drug/chemical diffusion through the skin. This area of theory, which is key to transdermal drug development and risk assessment of chemical exposure, has benefitted very directly from Brenner's contributions. In this as in other areas, Brenner's physicochemical insight, mathematical virtuosity, drive for fully justified analysis free of ad hoc assumptions, quest for generality, and impeccable exposition, have consistently elevated the level of theoretical understanding and presentation. We close with anecdotes showing how his personal qualities and warmth helped to impart high standards of rigor to generations of grateful research students. Authors are Johannes M. Nitsche, Ludwig C. Nitsche and Gerald B. Kasting.

  18. Obituary: Howard H. Lanning, 1946-2007

    NASA Astrophysics Data System (ADS)

    Wade, Richard A.; MacConnell, D. Jack

    2009-01-01

    Howard H. Lanning died 20 December 2007 in Tucson, Arizona. He was a Software Quality Assurance Engineer for the Data Products Program at the National Optical Astronomy Observatory [NOAO] in Tucson, having returned to his native West after twenty years at the Space Telescope Science Institute [STScI] in Baltimore, Maryland. Throughout his career in roles supporting other astronomers, Howard steadily and persistently worked at his own research programs, most notably the identification and study of UV-bright sources in the Sandage Two-Color Survey of the Galactic Plane. Minor planet 2000 QJ248 has been named (61913) Lanning in recognition of his contributions. The son of James Clyde Lanning and Ethel Malan Lanning, Howard was born 26 May 1946, in Brawley, a small community near Calipatria, California. His parents worked for the local school district where his late father was Superintendent of Maintenance and Grounds and his mother was a school principal. After graduating from high school in a class of 52, Howard earned his A.A. degree at Imperial Valley Junior College, majoring in Astronomy and minoring in Mathematics. He completed his A.B. degree in Applied Arts and Sciences at San Diego State University [SDSU] in 1969 with the same major and minor. In 1974, he completed his M.S. in Astronomy at SDSU with a thesis on the period variation of the white-dwarf eclipsing binary BD +16∘516. During his time at SDSU Lanning was a Research Assistant/Observer at the former Hale Observatories, working on the optical identification of X-ray sources under the direction of Allan Sandage. His second refereed paper, the first of several finding lists of UV-bright stars arising from this work, appeared in 1973. Fellow students and faculty remember that he was a self-starter, initiating many observing projects of his own at Mount Laguna Observatory and encouraging others to do the same. For more than a decade Lanning was an observer and night assistant at Mount Wilson Observatory

  19. Dale Reed with model in front of M2-F1

    NASA Technical Reports Server (NTRS)

    1967-01-01

    Dale Reed with a model of the M2-F1 in front of the actual lifting body. Reed used the model to show the potential of the lifting bodies. He first flew it into tall grass to test stability and trim, then hand-launched it from buildings for longer flights. Finally, he towed the lifting-body model aloft using a powered model airplane known as the 'Mothership.' A timer released the model and it glided to a landing. Dale's wife Donna used a 9 mm. camera to film the flights of the model. Its stability as it glided--despite its lack of wings--convinced Milt Thompson and some Flight Research Center engineers including the center director, Paul Bikle, that a piloted lifting body was possible. The lifting body concept evolved in the mid-1950s as researchers considered alternatives to ballistic reentries of piloted space capsules. The designs for hypersonic, wingless vehicles were on the boards at NASA Ames and NASA Langley facilities, while the US Air Force was gearing up for its Dyna-Soar program, which defined the need for a spacecraft that would land like an airplane. Despite favorable research on lifting bodies, there was little support for a flight program. Dryden engineer R. Dale Reed was intrigued with the lifting body concept, and reasoned that some sort of flight demonstration was needed before wingless aircraft could be taken seriously. In February 1962, he built a model lifting body based upon the Ames M2 design, and air-launched it from a radio controlled 'mothership.' Home movies of these flights, plus the support of research pilot Milt Thompson, helped pursuade the facilities director, Paul Bikle, to give the go-ahead for the construction of a full-scale version, to be used as a wind-tunnel model and possibly flown as a glider. Comparing lifting bodies to space capsules, an unofficial motto of the project was, 'Don't be Rescued from Outer Space--Fly Back in Style.' The construction of the M2-F1 was a joint effort by Dryden and a local glider manufacturer, the

  20. The correspondence of Thomas Dale (1700-1750): Botany in the transatlantic Republic of Letters.

    PubMed

    Cook, William J

    2012-03-01

    This paper seeks to provide a full account of the life and career of Dr. Thomas Dale (1700-1750), with particular reference to his botanical works and correspondence. Born in Hoxton, London, Dale studied medicine at Leiden and engaged fully in the social, literary and epistolary network in which botany was practised in eighteenth-century England. In 1730, however, Dale relocated to the British colonial port of Charles Town, South Carolina. Here he continued to engage in a transatlantic network of botanical exchange and discussion, corresponding on equal and reciprocal terms with his former colleagues in England. Where Dale differs from naturalists in South Carolina before him is that his motives for pursuing botany and for corresponding with English naturalists were located firmly in the New World. Such a conclusion forms a valuable, albeit small contribution to models for the development of national scientific cultures in the imperial world. Similarly, Dale's pursuit of botanical information in South Carolina provides a small amount of material with which to illustrate currently fashionable models for the mediated exchange and circulation of scientific knowledge.

  1. Perfluoroalkyl substances in Daling River adjacent to fluorine industrial parks: implication from industrial emission.

    PubMed

    Meng, Jing; Wang, Tieyu; Wang, Pei; Zhu, Zhaoyun; Li, Qifeng; Lu, Yonglong

    2015-01-01

    The pollution level and source of perfluoroalkyl substances (PFASs) in mainstream and tributary of Daling River in northeast China were investigated in present study. Concentrations of PFASs in surface water and sediment ranged from 4.6 to 3,410 ng/L and from 0.08 to 2.6 ng/g dry weight, respectively. The lowest levels of PFASs were found in vicinity of a drinking water source located in upstream of Daling River. Xihe tributary, which is adjacent to two local fluorine industrial parks, contained the highest level of PFASs. Short-chain PFASs, including perfluorobutanoic acid and perfluorobutane sulfonate, were of higher levels due to their emerging as alternative products for perfluorooctane sulfonate. High level of perfluorooctanoic acid was also found in Daling River. Based on these results, it can be concluded that the relatively severe pollutions of Xihe tributary were caused by long-term development of the two local fluorine industry parks.

  2. Antimatter/HiPAT Support Services

    NASA Technical Reports Server (NTRS)

    Lewis, Raymond A.

    2001-01-01

    Techniques were developed for trapping normal matter in the High Performance Antiproton Trap (HiPAT). Situations encountered included discharge phenomena, charge exchange and radial diffusion processes. It is important to identify these problems, since they will also limit the performance in trapping antimatter next year.

  3. A Poetry Workshop in Print: Pat Mora

    ERIC Educational Resources Information Center

    Hopkins, Lee Bennett

    2006-01-01

    After a successful career as a writer for adults, Pat Mora began creating books for children. Her first picture book, "Tomas and The Library Lady" (Knopf, 1997) is a tender story of a young migrant worker who unearths new worlds when he discovers the magic a public library holds. The text, cleverly interspersed with foreign words, became a…

  4. The Mythical Retention Chart and the Corruption of Dale's Cone of Experience

    ERIC Educational Resources Information Center

    Subramony, Deepak Prem; Molenda, Michael; Betrus, Anthony K.; Thalheimer, Will

    2014-01-01

    In response to the wide-scale proliferation of "the cone of learning"--a fanciful retention chart confounded with Dale's Cone of Experience--the authors make four major claims debunking this fantasy and provide documentary evidence to support these claims. The first claim is that the data in the mythical retention chart do not make…

  5. Previous Attempts to Debunk the Mythical Retention Chart and Corrupted Dale's Cone

    ERIC Educational Resources Information Center

    Subramony, Deepak Prem; Molenda, Michael; Betrus, Anthony K.; Thalheimer, Will

    2014-01-01

    Critics have been attempting to debunk the mythical retention chart at least since 1971. The earliest critics, David Curl and Frank Dwyer, were addressing just the retention data. Beginning around 2002, a new generation of critics has taken on the illegitimate combination of the retention chart and Edgar Dale's Cone of Experience--the corrupted…

  6. Comparing Rasch Calibration of Passage Reading Difficulty with Spache or Dale-Chall Passage Readability Estimates.

    ERIC Educational Resources Information Center

    Kidder, Steven J.

    The utility and precision of scaling 200 relatively short reading passages were analyzed using a Rasch-based measurement technique as compared to readability estimates using the Spache or Dale-Chall formulas. In addition, a regression model was specified for predicting the grade level of a text a student could literally comprehend based on…

  7. 76 FR 53964 - Dale J. Bingham, P.A.; Revocation of Registration

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-08-30

    ... registration. See David W. Wang, 72 FR 54297, 54298 (2007); Sheran Arden Yeates, 71 FR 39130, 39131 (2006); Dominick A. Ricci, 58 FR 51104, 51105 (1993); Bobby Watts, 53 FR 11919, 11920 (1988). Here, while... Enforcement Administration Dale J. Bingham, P.A.; Revocation of Registration On February 4, 2011, the...

  8. Howard Community College Enrollment by Census Tract, Fall 1991. Research Report Number 81.

    ERIC Educational Resources Information Center

    Heacock, Ronald C.; Jenkins, Jacqueline L.

    A study was conducted of students enrolled in fall 1991 at Howard Community College (HCC) in Columbia, Maryland, by matching student addresses to one of 29 populated census tracks in Howard County. The study sought to understand what part of Howard County students were coming from; whether HCC was serving more remote areas of the county; and…

  9. Composing a "National Negro Theater": Playwriting Courses at Howard University in the 1920s.

    ERIC Educational Resources Information Center

    Zaluda, Scott

    Educators today may find a historical review of the Howard Players at Howard University (Washington, D.C.) in the 1920s important because of its implicit commentary on what constitutes community. While the Howard Players are generally written about in terms of the development of an African-American theater, historians ought also to think of their…

  10. Bridges to Leadership 2000: Howard University Youth Leadership Program.

    ERIC Educational Resources Information Center

    Walker, Sylvia; Kaur, Satwant

    The purpose of the Bridges to Leadership 2000 program is to implement a service delivery model for economically disadvantaged and minority youth with disabilities, from ages 11 to 21. The program is conducted with the help of the Howard University Research and Training Center for Access to Rehabilitation and Economic Opportunity, and is being…

  11. Rise of Human Intelligence: Comments on Howard (1999).

    ERIC Educational Resources Information Center

    Gobet, Fernand; Campitelli, Guillermo; Waters, Andrew J.

    2002-01-01

    Finds several difficulties with the theory advanced by R. Howard and shows that alternative explanations relating to changes in the chess environment, including increased access to chess knowledge, offer better explanations for the increased presence of young players at top-level chess. (SLD)

  12. Howard Community College's 1992-1996 Graduates: A Trend Analysis.

    ERIC Educational Resources Information Center

    Howard Community Coll., Columbia, MD.

    This report describes and compares the demographic and academic characteristics of Fiscal Year (FY)92, FY93, FY94, FY95, and FY96 graduates of Howard Community College (HCC) in Maryland, and the respondents to graduate surveys. The purposes of these surveys were to collect data about the employment and educational experiences of alumni one year…

  13. Howard County Public High School Graduates at Maryland Campuses.

    ERIC Educational Resources Information Center

    Howard Community Coll., Columbia, MD. Office of Planning and Evaluation.

    This report presents results on the number of Maryland high school graduates who attend Howard Community College (HCC). The number of recent public high school graduates showing up at HCC increased from 384 (spring 1992 graduates) to 443 (spring 1997 graduates). HCC's share of the students who attend college in Maryland has ranged from 36-42% with…

  14. Statistical Profiles. Howard Community College. Research Report Number 40.

    ERIC Educational Resources Information Center

    Radcliffe, Susan K.; Reed, Cheryl L. B.

    This report summarizes 1980-84 data for Howard Community College (HCC) pertaining to student characteristics; credit instruction and degrees; credit-free instruction; HCC employees; and facilities and services. Part 1 provides data on full-time equivalent enrollments and projections; and enrollment by gender, attendance status, credit hours…

  15. Undue Certainty: Where Howard Zinn's "A People's History" Falls Short

    ERIC Educational Resources Information Center

    Wineburg, Sam

    2013-01-01

    Howard Zinn's "A People's History" of the United States has few peers among contemporary historical works. With more than 2 million copies in print, "A People's History" is more than a book. It is a cultural icon. While most historians aim to examine the full historical record, Zinn picks and chooses from it. Writing persuasively, he hides the…

  16. Evaluation of the Howard Hughes Science Grant Project, Year One

    ERIC Educational Resources Information Center

    Wolanin, Natalie; Wade, Julie

    2015-01-01

    The goal of the Howard Hughes Science Institute (HHMI) supported science program is to train one staff member to become a science lead within each of the elementary schools in the Montgomery County (Maryland) Public Schools (MCPS) district. The specific objectives of the first year of HHMI grant project were to: (1) provide approximately 20…

  17. Judicial Management: The Achievements of Chief Justice William Howard Taft.

    ERIC Educational Resources Information Center

    Post, Robert

    1998-01-01

    Illuminates the importance of Chief Justice William Howard Taft in creating the modern administrative role of the Chief Justice of the United States. Specifically, the article examines the Act of 14 September 1922 that Taft championed in Congress to give the Chief Justice better tools for managing the judiciary. (DSK)

  18. HOWARD UNIVERSITY STUDENTS AND THE COMMUNITY SERVICE PROJECT.

    ERIC Educational Resources Information Center

    LOWE, GILBERT A., JR.

    THIS REPORT DESCRIBES THE INVOLVEMENT OF HOWARD UNIVERSITY STUDENTS IN A FIVE-YEAR COMMUNITY SERVICE PROJECT IN THE SECOND PRECINCT IN WASHINGTON, D.C., BETWEEN 1961 AND 1966. THE PROJECT WAS PLANNED TO--(1) MOBILIZE AND ASSIST THE MORE THAN 160 CHURCHES IN THE AREA, (2) SURVEY THE NEEDS OF THE RESIDENTS AND HELP THEM SOLVE THEIR PROBLEMS, (3)…

  19. Mr Howard Goes to Washington--and Brings Home Victory

    ERIC Educational Resources Information Center

    Glover, Dennis

    2004-01-01

    In June 2002--seven months after the most divisive federal election campaign since 1975--Mr. John Howard went to Washington to be feted by the International Democratic Union--the worldwide association of centre-right political parties. So impressed were they by his against-the-odds victory that they elected him as their Chairman. In a White House…

  20. B & P Junction Substation & Interlocking Tower; Howard Street ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    B & P Junction Substation & Interlocking Tower; Howard Street Bridge. Baltimore, Baltimore City, MD. Sec. 1201, MP 95.85 - Northeast Railroad Corridor, Amtrak route between District of Columbia/Maryland state line & Maryland/Delaware state line, Baltimore, Independent City, MD

  1. A Review of Howard University's Financial Literacy Curriculum

    ERIC Educational Resources Information Center

    Lindsey-Taliefero, Debby; Kelly, Lynne; Brent, William; Price, Russell

    2011-01-01

    This article evaluates a financial literacy curriculum at the Howard University (HU) School of Business, by measuring the financial knowledge acquired after participating in a variety of programs. To evaluate the HU curriculum, the National Jump$tart Coalition (NJC) survey was administered to collect data on financial knowledge and demographic…

  2. Role of gibberellins in parthenocarpic fruit development induced by the genetic system pat-3/pat-4 in tomato.

    PubMed

    Fos, Mariano; Proaño, Karina; Nuez, Fernando; García-Martínez, José L.

    2001-04-01

    The role of gibberellins (GAs) in the induction of parthenocarpic fruit-set and growth by the pat-3/pat-4 genetic system in tomato (Lycopersicon esculentum Mill.) was investigated using wild type (WT; Cuarenteno) and a near-isogenic line derived from the German line RP75/59 (the source of pat-3/pat-4 parthenocarpy). Unpollinated WT ovaries degenerated but GA3 application induced parthenocarpic fruit growth. On the contrary, parthenocarpic growth of pat-3/pat-4 fruits, which occurs in the absence of pollination and hormone treatment, was not affected by applied GA3. Unpollinated pat-3/pat-4 fruit growth was negated by paclobutrazol, an inhibitor of ent-kaurene oxidase, and this inhibitory effect was negated by GA3. The quantification of the main GAs of the early 13-hydroxylation pathway (GA1, GA8, GA19, GA20, GA29 and GA44) in unpollinated ovaries at 3 developmental stages (flower bud, FB; pre-anthesis, PR; and anthesis, AN), by gas chromatography-selected ion monitoring, showed that the concentration of most of them was higher in pat-3/pat-4 than in WT ovaries at PR and AN stages. The concentration of GA1, suggested previously to be the active GA in tomate, was 2-4 times higher. Unpollinated pat-3/pat-4 ovaries at FB, PR and AN stages also contained relatively high amounts (5-12 ng g-1) of GA3, a GA found at less than 0.5 ng g-1 in WT ovaries. It is concluded that the mutations pat-3/pat-4 may induce natural facultative parthenocarpy capacity in tomato by increasing the concentration of GA1 and GA3 in the ovaries before pollination. PMID:11299021

  3. Role of gibberellins in parthenocarpic fruit development induced by the genetic system pat-3/pat-4 in tomato.

    PubMed

    Fos, Mariano; Proaño, Karina; Nuez, Fernando; García-Martínez, José L.

    2001-04-01

    The role of gibberellins (GAs) in the induction of parthenocarpic fruit-set and growth by the pat-3/pat-4 genetic system in tomato (Lycopersicon esculentum Mill.) was investigated using wild type (WT; Cuarenteno) and a near-isogenic line derived from the German line RP75/59 (the source of pat-3/pat-4 parthenocarpy). Unpollinated WT ovaries degenerated but GA3 application induced parthenocarpic fruit growth. On the contrary, parthenocarpic growth of pat-3/pat-4 fruits, which occurs in the absence of pollination and hormone treatment, was not affected by applied GA3. Unpollinated pat-3/pat-4 fruit growth was negated by paclobutrazol, an inhibitor of ent-kaurene oxidase, and this inhibitory effect was negated by GA3. The quantification of the main GAs of the early 13-hydroxylation pathway (GA1, GA8, GA19, GA20, GA29 and GA44) in unpollinated ovaries at 3 developmental stages (flower bud, FB; pre-anthesis, PR; and anthesis, AN), by gas chromatography-selected ion monitoring, showed that the concentration of most of them was higher in pat-3/pat-4 than in WT ovaries at PR and AN stages. The concentration of GA1, suggested previously to be the active GA in tomate, was 2-4 times higher. Unpollinated pat-3/pat-4 ovaries at FB, PR and AN stages also contained relatively high amounts (5-12 ng g-1) of GA3, a GA found at less than 0.5 ng g-1 in WT ovaries. It is concluded that the mutations pat-3/pat-4 may induce natural facultative parthenocarpy capacity in tomato by increasing the concentration of GA1 and GA3 in the ovaries before pollination.

  4. PAT-1 safety analysis report addendum.

    SciTech Connect

    Weiner, Ruth F.; Schmale, David T.; Kalan, Robert J.; Akin, Lili A.; Miller, David Russell; Knorovsky, Gerald Albert; Yoshimura, Richard Hiroyuki; Lopez, Carlos; Harding, David Cameron; Jones, Perry L.; Morrow, Charles W.

    2010-09-01

    The Plutonium Air Transportable Package, Model PAT-1, is certified under Title 10, Code of Federal Regulations Part 71 by the U.S. Nuclear Regulatory Commission (NRC) per Certificate of Compliance (CoC) USA/0361B(U)F-96 (currently Revision 9). The purpose of this SAR Addendum is to incorporate plutonium (Pu) metal as a new payload for the PAT-1 package. The Pu metal is packed in an inner container (designated the T-Ampoule) that replaces the PC-1 inner container. The documentation and results from analysis contained in this addendum demonstrate that the replacement of the PC-1 and associated packaging material with the T-Ampoule and associated packaging with the addition of the plutonium metal content are not significant with respect to the design, operating characteristics, or safe performance of the containment system and prevention of criticality when the package is subjected to the tests specified in 10 CFR 71.71, 71.73 and 71.74.

  5. Dr. Howard Jones, Jr. (1910-2015): In Memoriam

    PubMed Central

    Oehninger, S.; Kruger, T.F.

    2015-01-01

    We honor the life of Howard W. Jones, Jr., MD, 104, a pioneer and visionary in reproductive medicine, a dedicated mentor, internationally renowned gynecological surgeon, and the father of in vitro fertilization IVF) in the United States. Dr. Jones founded the Jones Institute for Reproductive Medicine, in Norfolk, Virginia, at Eastern Virginia Medical School, in 1983. Today, IVF has grown exponentially not only in its direct application but also in the development of newer techniques would have been unimaginable a couple of decades ago. Throughout the years Dr. Howard has been a teacher and a mentor to many around the world. To us he also was a friend, a supporter at the time of difficult decisions, someone who would inspire hope, ignite the imagination, and instill a love of learning and untangling some of the complexities of the field, even of the human condition. His leadership, vision, knowledge, enthusiasm and inquisitive mind will be missed.

  6. Differential expression of proton-assisted amino acid transporters (PAT[1] and PAT[2]) in tissues of neonatal pigs

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The PATs have been identified as growth-regulatory nutrient sensors in Drosophila and as activators of mammalian target of rapamycin (mTOR) in mammalian cell cultures. These studies suggest that, beyond their classical function as transporters of simple amino acids (AA), the PATs act as tranceptors,...

  7. The 3' overhangs at Tetrahymena thermophila telomeres are packaged by four proteins, Pot1a, Tpt1, Pat1, and Pat2.

    PubMed

    Premkumar, Vidjaya Letchoumy; Cranert, Stacey; Linger, Benjamin R; Morin, Gregg B; Minium, Sasha; Price, Carolyn

    2014-02-01

    Although studies with the ciliate Tetrahymena thermophila have played a central role in advancing our understanding of telomere biology and telomerase mechanisms and composition, the full complement of Tetrahymena telomere proteins has not yet been identified. Previously, we demonstrated that in Tetrahymena, the telomeric 3' overhang is protected by a three-protein complex composed of Pot1a, Tpt1, and Pat1. Here we show that Tpt1 and Pat1 associate with a fourth protein, Pat2 (Pot1 associated Tetrahymena 2). Mass spectrometry of proteins copurifying with Pat1 or Tpt1 identified peptides from Pat2, Pot1a, Tpt1, and Pat1. The lack of other proteins copurifying with Pat1 or Tpt1 implies that the overhang is protected by a four-protein Pot1a-Tpt1-Pat1-Pat2 complex. We verified that Pat2 localizes to telomeres, but we were unable to detect direct binding to telomeric DNA. Cells depleted of Pat2 continue to divide, but the telomeres exhibit gradual shortening. The lack of growth arrest indicates that, in contrast to Pot1a and Tpt1, Pat2 is not required for the sequestration of the telomere from the DNA repair machinery. Instead, Pat2 is needed to regulate telomere length, most likely by acting in conjunction with Pat1 to allow telomerase access to the telomere.

  8. Becoming respectable: T. Dale Stewart and the acceptance of forensic anthropology in the academic community.

    PubMed

    Kennedy, K A

    2000-03-01

    Before World War II, forensic anthropology was of peripheral interest to a few anthropologists willing to assist in investigations by law enforcement agencies. A strong bias that "police work" was unbecoming to the scholarly pursuits of academics persisted into the post-war years. Changes took place as a consequence of T. Dale Stewart's case work in the identification of human remains with the FBI from 1943 to 1969, his directorship of the National Museum of Natural History (Smithsonian Institution) beginning in 1962, and his work with the Armed Forces after 1948. This paper discusses the historic period of transition of attitudes and practices in the contexts of Stewart's contributions and the cases and teaching programs of one of his contemporaries. Theodore D. McCown at the University of California at Berkeley, during the period of 1939 to 1969. The establishment of the Physical Anthropology Section within the American Academy of Forensic Sciences in 1972 and the creation of the T. Dale Stewart award for distinguished service in forensic anthropology advanced those laboratory research programs and medical-legal investigations conducted by present-day forensic anthropologists. PMID:10782943

  9. Becoming respectable: T. Dale Stewart and the acceptance of forensic anthropology in the academic community.

    PubMed

    Kennedy, K A

    2000-03-01

    Before World War II, forensic anthropology was of peripheral interest to a few anthropologists willing to assist in investigations by law enforcement agencies. A strong bias that "police work" was unbecoming to the scholarly pursuits of academics persisted into the post-war years. Changes took place as a consequence of T. Dale Stewart's case work in the identification of human remains with the FBI from 1943 to 1969, his directorship of the National Museum of Natural History (Smithsonian Institution) beginning in 1962, and his work with the Armed Forces after 1948. This paper discusses the historic period of transition of attitudes and practices in the contexts of Stewart's contributions and the cases and teaching programs of one of his contemporaries. Theodore D. McCown at the University of California at Berkeley, during the period of 1939 to 1969. The establishment of the Physical Anthropology Section within the American Academy of Forensic Sciences in 1972 and the creation of the T. Dale Stewart award for distinguished service in forensic anthropology advanced those laboratory research programs and medical-legal investigations conducted by present-day forensic anthropologists.

  10. Pat Thiel talks about Nobel Prize winner Dan Shechtman

    ScienceCinema

    Thiel, Pat

    2016-07-12

    Ames Laboratory senior scientist and Iowa State University Distinguished Professor of Chemistry Pat Thiel talks about her friend and colleague Dan Shechtman who received the 2011 Nobel Prize for Chemistry.

  11. High School Juniors: Their Career Aspirations, Educational Plans, and Perceptions of Howard Community College.

    ERIC Educational Resources Information Center

    Wenckowski, Charlene

    In order to be able to more effectively serve the Howard County high school graduate, Howard Community College (HCC) surveyed high school juniors in spring 1978 as to their career interests and educational plans, their attitudes toward the college and its recruitment efforts, and their reading and listening habits (for advertising purposes).…

  12. Analysis of Employee Supply and Demand in Howard County--A Pilot Approach.

    ERIC Educational Resources Information Center

    Howard Coll. at Big Spring, TX.

    A survey developed by the Occupational Education Division of Howard University to determine employee supply and demand in Howard County, Texas, involved both employees and employers. Employer data were compiled from interviews with 319 out of a possible 409 employers who employed three or more people. Conclusions and recommendations for the…

  13. Overexpression of patA and patB, Which Encode ABC Transporters, Is Associated with Fluoroquinolone Resistance in Clinical Isolates of Streptococcus pneumoniae▿

    PubMed Central

    Garvey, Mark I.; Baylay, Alison J.; Wong, Ryan L.; Piddock, Laura J. V.

    2011-01-01

    Fifty-seven clinical isolates of Streptococcus pneumoniae were divided into four groups based on their susceptibilities to the fluoroquinolones ciprofloxacin and norfloxacin and the dyes ethidium bromide and acriflavine. Comparative reverse transcription-PCR was used to determine the level of expression of the genes patA and patB, which encode putative ABC transporters. Overexpression was observed in 14 of the 15 isolates that were resistant to both fluoroquinolones and dyes and in only 3 of 24 of those resistant to fluoroquinolones only. Isolates overexpressing patA and patB accumulated significantly less of the fluorescent dye Hoechst 33342 than wild-type isolates, suggesting that PatA and PatB are involved in efflux. Inactivation of patA and patB by in vitro mariner mutagenesis conferred hypersusceptibility to ethidium bromide and acriflavine in all isolates tested and lowered the MICs of ciprofloxacin in the patAB-overproducing and/or fluoroquinolone-resistant isolates. These data represent the first observation of overexpression of patA and patB in clinical isolates and show that PatA and PatB play a clinically relevant role in fluoroquinolone resistance. PMID:20937787

  14. Howard Florey and research on the cerebral circulation.

    PubMed

    Todman, Donald

    2008-06-01

    Howard Florey is best known as the scientist who developed penicillin and ushered in the modern antibiotic era. As an experimental pathologist he founded one of the leading schools of experimental pathology at Oxford and his achievements established him as one of the foremost medical scientists of the twentieth century. Florey's ambition and drive toward medical research commenced in the years 1922-1925 when he was a Rhodes scholar at Oxford. His mentor was the neurophysiologist and Nobel Laureate, Sir Charles Sherrington who directed him in neuroscience research. Florey's initial studies on the cerebral circulation represent an original contribution to medical knowledge and highlight his remarkable scientific method. The mentorship and close personal relationship with Sherrington was a crucial factor in Florey's early research career.

  15. The Planetary and Eclipse Oil Paintings of Howard Russell Butler

    NASA Astrophysics Data System (ADS)

    Pasachoff, Jay M.; Olson, R. M.

    2013-10-01

    The physics-trained artist Howard Russell Butler (1856-1934) has inspired many astronomy students through his planetary and eclipse paintings that were long displayed at the Hayden Planetarium in New York, the Fels Planetarium at the Franklin Institute in Philadelphia, and the Buffalo Museum of Science. We discuss not only the eclipse triptychs (1918, 1923, and 1925) at each of those institutions but also his paintings of Mars as seen from Phobos and from Deimos (with landscapes of those moons in the foreground depicted in additional oils hung at Princeton University) and the Earth from our Moon. We also describe his involvement with astronomy and his unique methodology that allowed him to surpass the effects then obtainable with photography, as well as his inclusion in a U.S. Naval Observatory eclipse expedition in 1918, as well as his auroral, solar-prominence, and 1932-eclipse paintings.

  16. Macquarie Island: the introduction of the European rabbit flea Spilopsyllus cuniculi (Dale) as a possible vector for myxomatosis

    PubMed Central

    Sobey, W. R.; Adams, K. M.; Johnston, G. C.; Gould, L. R.; Simpson, K. N. G.; Keith, K.

    1973-01-01

    The European rabbit flea Spilopsyllus cuniculi (Dale) was first released on Macquarie Island in December 1968. The flea has survived and bred on the island and about 30% of the rabbits sampled from the original release area in January 1972 were flea-infested. ImagesPlate 1Plate 1 PMID:4515880

  17. Cosmopolitanism and Our Descriptions of Ethics and Ontology: A Response to Dale Snauwaert's "The Ethics and Ontology of Cosmopolitanism"

    ERIC Educational Resources Information Center

    Hansen, David T.

    2010-01-01

    In recent years scholars across the humanities and social sciences have revitalized the ancient concept of cosmopolitanism. Dale Snauwaert illuminates why this is so in his thoughtful article on what it might mean to educate for a shared humanity. Snauwaert shows why many people find so-called "realism" an unsatisfactory political and moral…

  18. Fine mapping of the parthenocarpic fruit ( pat) mutation in tomato.

    PubMed

    Beraldi, D; Picarella, M E; Soressi, G P; Mazzucato, A

    2004-01-01

    The parthenocarpic fruit ( pat) gene of tomato is a recessive mutation conferring parthenocarpy, which is the capability of a plant to set seedless fruits in the absence of pollination and fertilization. Parthenocarpic mutants offer a useful method to regulate fruit production and a suitable experimental system to study ovary and fruit development. In order to map the Pat locus, two populations segregating from the interspecific cross Lycopersicon esculentum x Lycopersicon pennellii were grown, and progeny plants were classified as parthenocarpic or wild-type by taking into account some characteristic aberrations affecting mutant anthers and ovules. Through bulk segregant analysis, we searched for both random and mapped AFLPs linked to the target gene. In this way, the Pat locus was assigned to the long arm of chromosome 3, as also confirmed by the analysis of a set of L. pennellii substitution and introgression lines. Afterwards, the Pat position was refined by using simple sequence repeats (SSRs) and conserved ortholog set (COS) markers mapping in the target region. The tightest COSs were converted into CAPS or SCAR markers. At present, two co-dominant SCAR markers encompassing a genetic window of 1.2 cM flank the Pat locus. Considering that these markers are orthologous to Arabidopsis genes, a positional cloning exploiting the tomato- Arabidopsis microsynteny seems to be a short-term objective. PMID:14564391

  19. Multivariate PAT solutions for biopharmaceutical cultivation: current progress and limitations.

    PubMed

    Mercier, Sarah M; Diepenbroek, Bas; Wijffels, Rene H; Streefland, Mathieu

    2014-06-01

    Increasingly elaborate and voluminous datasets are generated by the (bio)pharmaceutical industry and are a major challenge for application of PAT and QbD principles. Multivariate data analysis (MVDA) is required to delineate relevant process information from large multi-factorial and multi-collinear datasets. Here the key role of MVDA for industrial (bio)process data is discussed, with a focus on progress and limitations of MVDA as a PAT solution for biopharmaceutical cultivation processes. MVDA based models were proven useful and should be routinely implemented for bioprocesses. It is concluded that although the highest level of PAT with process control within its design space in real-time during manufacturing is not reached yet, MVDA will be central to reach this ultimate objective for cell cultivations. PMID:24732022

  20. Multivariate PAT solutions for biopharmaceutical cultivation: current progress and limitations.

    PubMed

    Mercier, Sarah M; Diepenbroek, Bas; Wijffels, Rene H; Streefland, Mathieu

    2014-06-01

    Increasingly elaborate and voluminous datasets are generated by the (bio)pharmaceutical industry and are a major challenge for application of PAT and QbD principles. Multivariate data analysis (MVDA) is required to delineate relevant process information from large multi-factorial and multi-collinear datasets. Here the key role of MVDA for industrial (bio)process data is discussed, with a focus on progress and limitations of MVDA as a PAT solution for biopharmaceutical cultivation processes. MVDA based models were proven useful and should be routinely implemented for bioprocesses. It is concluded that although the highest level of PAT with process control within its design space in real-time during manufacturing is not reached yet, MVDA will be central to reach this ultimate objective for cell cultivations.

  1. Howard Russell Butler's Oil Paintings of Solar Eclipses and Prominences

    NASA Astrophysics Data System (ADS)

    Pasachoff, Jay M.; Olson, Roberta J. M.

    2014-06-01

    Howard Russell Butler (1856-1934) was invited to join the US Naval Observatory expedition to the total solar eclipse of 1918 because of his ability to paint astronomical phenomena based on quickly-made notes about spatial and color details. His giant triptych of the total eclipses of 1918, 1923, and 1925 was proposed for a never-built astronomical center at the American Museum of Natural History and wound up at their Hayden Planetarium when it was constructed in the mid-1930s. Half-size versions are installed at the Fels Planetarium at the Franklin Institute in Philadelphia and at the Firestone Library of Princeton University, whose newly conserved canvases were recently hung; the Buffalo Museum of Science has another half-size version in storage. We discuss not only the eclipse triptychs but also the series of large oil paintings he made of solar prominences (in storage at the American Museum of Natural History) and of his 1932-eclipse and other relevant works.JMP was supported for this work in part by Division III Discretionary Funds and the Brandi Fund of Williams College. His current eclipse research is supported by grants AGS-1047726 from the Solar Research Program of the Atmospheric and Geospace Sciences Division of NSF and 9327-13 from the Committee for Research and Exploration of the National Geographic Society.

  2. Howard University Energy Expert Systems Institute Summer Program (EESI)

    NASA Technical Reports Server (NTRS)

    Momoh, James A.; Chuku, Arunsi; Abban, Joseph

    1996-01-01

    Howard University, under the auspices of the Center for Energy Systems and Controls runs the Energy Expert Systems Institute (EESI) summer outreach program for high school/pre-college minority students. The main objectives are to introduce precollege minority students to research in the power industry using modern state-of-the-art technology such as Expert Systems, Fuzzy Logic and Artificial Neural Networks; to involve minority students in space power management, systems and failure diagnosis; to generate interest in career options in electrical engineering; and to experience problem-solving in a teamwork environment consisting of faculty, senior research associates and graduate students. For five weeks the students are exposed not only to the exciting experience of college life, but also to the inspiring field of engineering, especially electrical engineering. The program consists of lectures in the fundamentals of engineering, mathematics, communication skills and computer skills. The projects are divided into mini and major. Topics for the 1995 mini projects were Expert Systems for the Electric Bus and Breast Cancer Detection. Topics on the major projects include Hybrid Electric Vehicle, Solar Dynamics and Distribution Automation. On the final day, designated as 'EESI Day' the students did oral presentations of their projects and prizes were awarded to the best group. The program began in the summer of 1993. The reaction from the students has been very positive. The program also arranges field trips to special places of interest such as the NASA Goddard Space Center.

  3. The Solar Eclipse Mural Series by Howard Russell Butler

    NASA Astrophysics Data System (ADS)

    Pasachoff, J. M.; Olson, R. J. M.

    2016-01-01

    There is a rich trove of astronomical phenomena in works of art by artists from the greater New York area, a trend that is even more pronounced in the oeuvres of New York City residents through the present day. A case in point is the trio of oil paintings by artist (and former physics professor) Howard Russell Butler depicting total solar eclipses in 1918, 1923, and 1925 that are based on his own observations. They were long displayed in the former art-deco building of the Hayden Planetarium of the American Museum of Natural History, the location of this conference. (The Museum also has nine other Butler paintings, none of which are currently exhibited.) Since the eclipse paintings have been in storage for many years, these once famous works are now virtually forgotten. Based on our research as an astronomer who has seen sixty-two solar eclipses and an art historian who has written extensively about astronomical imagery, we will discuss Butler's Solar Eclipse Triptych to explore its place in the history of astronomical imaging.

  4. Stable phantoms for characterization of photoacoustic tomography (PAT) systems

    NASA Astrophysics Data System (ADS)

    Bohndiek, Sarah E.; Van de Sompel, Dominique; Bodapati, Sandhya; Kothapalli, Sri Rajasekhar; Gambhir, Sanjiv S.

    2013-02-01

    Photoacoustic tomography (PAT) is an emerging modality that combines the high contrast of optical imaging, with the spatial resolution and penetration depth of ultrasound, by exploiting the photoacoustic effect. As with any new imaging modality, reliable physical phantoms are needed to: calibrate instruments; validate performance; optimize signal-to-noise; perform routine quality control; and compare systems. Phantom materials for testing small animal PAT systems should also mimic both the optical and acoustic properties of soft tissue, while for calibration purposes should be resistant to degradation over long time periods. We show here that polyvinyl chloride plastisol (PVCP) phantoms enable calibration and performance validation using two PAT systems with distinct designs (Visualsonics Vevo LAZR and Endra Nexus 128) across a wavelength range of 680 nm - 950 nm. Inclusions between 2 and 3.2 mm in diameter were fabricated from PVCP using a range of dye concentrations (0 % to 0.256 % Black Plastic Color, BPC) in a custom mold. A calibration phantom was imaged repeatedly on both systems, over time scales of minutes, hours and days, to assess system stability. Both systems demonstrated good reproducibility over time, with the coefficient of variation in the measured signal-to-noise ratio (SNR) being less than 15% over the course of 30 days. Imaging performance was optimized by plotting SNR as a function of different system parameters. The visualization of objects embedded in optically absorbing and scattering backgrounds was also assessed. PVCP is easy to work with and provides stable phantoms for assessing PAT system performance.

  5. The PATS Project: A State of Tennessee School Reform Initiative.

    ERIC Educational Resources Information Center

    Butler, E. Dean; And Others

    The first-year results of a Tennessee college-school partnership program to implement a data-based school improvement change model--the Positive Attitudes in Tennessee Schools Project (PATS)--are summarized in this report. Two major goals of the first year (1989-90) included assessing and improving school climate and enhancing student and teacher…

  6. Project Achieve Transition Services (PATS), 1993-94. OER Report.

    ERIC Educational Resources Information Center

    Goldberg, Phyllis

    Project Achieve Transition Services (PATS) is a four-year high school attendance improvement, dropout prevention project which targets late-entry students consisting primarily of immigrants, transfers, and long-term absentees. The program uses a case management approach to provide support services, instructional enhancements, and family outreach…

  7. 10. GLASS, SCHNEIDER & REZNER BRIDGE PATENT MODEL, PAT. NO. ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    10. GLASS, SCHNEIDER & REZNER BRIDGE PATENT MODEL, PAT. NO. 71, 868, DECEMBER 10, 1867. THIS MODEL IS ONE OF A FEW THAT SURVIVED THE PATENT OFFICE FIRE OF 1877. IF REPRODUCED, CREDIT SHOULD BE GIVEN TO THE SMITHSONIAN INSTITUTION, NEGATIVE NO. 48660-D. - White Bowstring Arch Truss Bridge, Spanning Yellow Creek at Cemetery Drive (Riverside Drive), Poland, Mahoning County, OH

  8. Pat Thiel talks about attending the Nobel Prize Award Ceremony

    ScienceCinema

    Thiel, Pat

    2016-07-12

    Pat Thiel, Ames Laboratory senior scientist and Iowa State University Distinguished Professor of Chemistry, was invited to be a guest at the ceremony on December 10th, in Stockholm, Sweden, where Danny Shechtman, Ames Laboratory scientist, received the 2011 Nobel Prize in Chemistry. Following her return to the Lab, Thiel shared some of her recollections of the momentous event.

  9. Identification of a Mutation Associated with Fatal Foal Immunodeficiency Syndrome in the Fell and Dales Pony

    PubMed Central

    Fox-Clipsham, Laura Y.; Carter, Stuart D.; Goodhead, Ian; Hall, Neil; Knottenbelt, Derek C.; May, Paul D. F.; Ollier, William E.; Swinburne, June E.

    2011-01-01

    The Fell and Dales are rare native UK pony breeds at risk due to falling numbers, in-breeding, and inherited disease. Specifically, the lethal Mendelian recessive disease Foal Immunodeficiency Syndrome (FIS), which manifests as B-lymphocyte immunodeficiency and progressive anemia, is a substantial threat. A significant percentage (∼10%) of the Fell ponies born each year dies from FIS, compromising the long-term survival of this breed. Moreover, the likely spread of FIS into other breeds is of major concern. Indeed, FIS was identified in the Dales pony, a related breed, during the course of this work. Using a stepwise approach comprising linkage and homozygosity mapping followed by haplotype analysis, we mapped the mutation using 14 FIS–affected, 17 obligate carriers, and 10 adults of unknown carrier status to a ∼1 Mb region (29.8 – 30.8 Mb) on chromosome (ECA) 26. A subsequent genome-wide association study identified two SNPs on ECA26 that showed genome-wide significance after Bonferroni correction for multiple testing: BIEC2-692674 at 29.804 Mb and BIEC2-693138 at 32.19 Mb. The associated region spanned 2.6 Mb from ∼29.6 Mb to 32.2 Mb on ECA26. Re-sequencing of this region identified a mutation in the sodium/myo-inositol cotransporter gene (SLC5A3); this causes a P446L substitution in the protein. This gene plays a crucial role in the regulatory response to osmotic stress that is essential in many tissues including lymphoid tissues and during early embryonic development. We propose that the amino acid substitution we identify here alters the function of SLC5A3, leading to erythropoiesis failure and compromise of the immune system. FIS is of significant biological interest as it is unique and is caused by a gene not previously associated with a mammalian disease. Having identified the associated gene, we are now able to eradicate FIS from equine populations by informed selective breeding. PMID:21750681

  10. Dynamic recrystallization mechanisms and their transition in the Daling Thrust (DT) zone, Darjeeling-Sikkim Himalaya

    NASA Astrophysics Data System (ADS)

    Ghosh, Subhajit; Bose, Santanu; Mandal, Nibir; Dasgupta, Sujoy

    2016-04-01

    The Daling Thrust (DT) delineates a zone of intense shear localization in the Lesser Himalayan Sequence (LHS) of the Darjeeling-Sikkim Himalaya. From microstructural studies of deformed quartzite samples, we show a transition in the dynamic recrystallization mechanism with increasing distance from the DT, dominated by grain boundary bulging (BLG) recrystallization closest to the DT, and progressively replaced by sub-grain rotation (SGR) recrystallization away from the thrust. The transition is marked by a characteristic variation in the fractal dimension (D) of grain boundaries, estimated from the area-perimeter method. For the BLG regime, D ≈ 1.046, which decreases significantly to a value as low as 1.025 for the SGR regime. Using the available thermal data for BLG and SGR recrystallization, we infer increasing deformation temperatures away from the DT in the hanging wall. Based on the quartz piezometer our estimates reveal strong variations in the flow stress (59.00 MPa to 16.00 MPa) over a distance of ~ 1.2 km from the DT. Deformation mechanism maps constructed for different temperatures indicate that the strain rates (10- 12 S- 1 to 10- 14 S- 1) comply with the geologically possible range. Finally, we present a mechanical model to provide a possible explanation for the cause of stress intensification along the DT.

  11. Health assessment for Van Dale Junkyard, Marietta, Ohio, Region 5. CERCLIS No. OHD980794606. Preliminary report

    SciTech Connect

    Not Available

    1989-03-17

    The Van Dale Junkyard Site (VDJ) is listed on the National Priorities List. The 10-acre site is located on a ridge near Duck Creek, a tributary of the Ohio River, in Marietta (Washington County), Ohio. VDJ is a licensed junkyard containing hundreds of drums containing waste dyes and organic solvents. Preliminary on-site sludge sampling results have identified various volatile organic compounds (VOCs). They include: chlorobenzene (13 ppm), toluene (1 ppm), trichloroethane (30 ppm), styrene (1 ppm), methyl ethyl ketone (10 ppm), methyl isobutyl ketone (10 ppm), xylene (1 ppm), and trimethyl benzene (2 ppm). On-site soil and off-site sediment sampling results have identified various polynuclear aromatic hydrocarbons (PAHs), phthalates, and other VOCs. Physical hazards were not reported. The site is considered to be of potential public health concern because of the risk caused by the likelihood of human exposure to hazardous substances. Direct contact and possible ingestion and inhalation of waste contaminants in drums and contaminated soil by on-site employees, persons having business on-site, and possible trespassers.

  12. Dale Schumacher on strategic quality planning and research. Interview by Joann Grenovich-Richards.

    PubMed

    Schumacher, Dale

    2003-01-01

    Dale N. Schumacher, MD MPH, is president of the Rockburn Institute, a not-for-profit health services research and consulting organization. Dr. Schumacher has 25 years' experience in the fields of medicine, health services research, and consulting. He spent 3 years in the U.S. Public Health Service, where he coordinated the development of the Sickness Index Profile, an early health status measure. Dr. Schumacher also helped establish the Center for Hospital Finance and Management at The Johns Hopkins Medical Institutions, where he was an associate director. He has held senior management positions or consulted with the Sisters of Mercy Health Corporation, Holy Cross Health System, Alexian Brothers Health System, Catholic Medical Center, and Northwestern Healthcare. Currently, he is senior medical advisor to the National Business Coalition on Health and serves as clinical informatics officer for Crozer-Keystone Health System in Philadelphia. He is a board member at Kaleida Health System, Buffalo, NY. Dr. Schumacher has written more than 40 publications in the areas of medical education, medical care, quality-of-care assessment, healthcare data analysis, and reliability of chart-review data. Dr. Schumacher earned his medical degree from the University of Illinois and his master of public health degree from The Johns Hopkins University School of Hygiene and Public Health, and was a postdoctoral fellow there in the departments of health services administration and epidemiology. He trained at The Johns Hopkins/Baltimore City Hospitals and St. Agnes Hospital. Dr. Schumacher is board certified in internal medicine. PMID:14606208

  13. The "in-between writer": Howard Stein, PhD, professor emeritus, psycho-analytic anthropologist, poet, educator, transdisciplinary scholar.

    PubMed

    Reilly, Jo Marie

    2016-09-01

    This commentary reflects the professional life story of a prolific and well-published poet, Howard Stein. An anthropologist by training, Howard's poetry is well known and well respected by family physicians. It is within family medicine that Howard found his professional home, and in his 45-plus-year career he has shared the value of "patient story"; the value of the doctor-patient relationship; and the art of listening deeply to self, colleagues, and patients. This commentary offers a tribute to Howard's professional life and his contributions to family and narrative medicine. (PsycINFO Database Record PMID:27632548

  14. Use of modified Fraser's stain in Promoting Activity Test (PAT).

    PubMed

    Borràs, M

    1988-09-01

    The Promoting Activity Test (PAT) requires a staining procedure that allows rapid, accurate and reliable counting of mitotic figures. We propose use of Fraser's kernechtrot-crystal violet technique, but eliminating the picric-alcoholic differentiation to avoid fading. This modified protocol gives higher mitotic counts in adult mouse adrenal cortex than the hematoxylin-eosin originally used, especially with respect to less conspicuous prophases. PMID:2464217

  15. Rapid Response to the Howard Hanson Dam Crisis

    NASA Astrophysics Data System (ADS)

    Ralph, F. M.; Carter, G.; White, A.; Neiman, P. J.; King, C.; Jankov, I.; Colman, B.; Cook, K.; Buehner, T.

    2010-12-01

    Dedicated in 1962, the Howard A. Hanson Dam (HHD) brought necessary flood relief to the Green River Valley in the Metropolitan Area of Seattle, Washington, and opened the way for increased valley development. For example, the flood damage prevented by HHD during the extreme precipitation event in early January 2009 is estimated to be about $4 billion. However, following the record high level of water behind HHD caused by this event, the U.S. Army Corps of Engineers (ACE) became concerned about the Dam’s safety. Despite short-term measures to improve HHD during 2009, the chance for a significant Green River Valley flood event was estimated by the ACE to be 1 in 25 for the 2009/10 winter season. In response to this elevated risk, NOAA organized a coordinated effort across research and forecast operations to implement new observations, modeling and dissemination tools, and knowledge of the role of atmospheric rivers (ARs) in producing extreme precipitation, prototyped in California within NOAA’s Hydrometeorology Testbed (HMT; hmt.noaa.gov). Fortunately, although seasonal observed precipitation (October 2009 through March 2010) in the Seattle area was slightly greater than normal (e.g., 112% of normal at the Seattle National Weather Service Forecast Office), there were no threatening floods observed along the Green River. This outcome was influenced by a synoptic pattern that was progressive, i.e., storms did not stall over this vulnerable watershed, which reduced the chance for an extensive (time and space) extreme event. More than a decade of West Coast winter storm research conducted primarily in California by NOAA’s Earth System Research Laboratory/Physical Sciences Division (ESRL/PSD) has identified atmospheric rivers (ARs), narrow regions of enhanced water vapor transport, as the culprits that cause extreme precipitation events, such as the January 2009 event that stressed HHD. ESRL/PSD extended this AR research to the coast of Washington by deploying a

  16. Overview of the High Performance Antiproton (HiPAT) Experiment

    NASA Technical Reports Server (NTRS)

    Martin, James J.; Sims, William H.; Chakrabarti, Suman; Pearson, Boise; Fant, Wallace E.; Lewis, Raymond A.; Rodgers, Stephen (Technical Monitor)

    2002-01-01

    The annihilation of matter with antimatter represents the highest energy density of any known reaction, producing 10(exp 8) MJ/g, approximately 10 orders of magnitude more energy per unit mass than chemical based combustion. To take the first step towards using this energy for propulsion applications the NASA MSFC Propulsion Research Center (PRC) has initiated a research activity examining the storage of low energy antiprotons. Storage was identified as a key enabling technology since it builds the experience base necessary to understand the handling of antiprotons for virtually all utilization and high-density storage concepts. To address this need, a device referred to as the High Performance Antiproton Trap (HiPAT) is under development at the NASA MSFC PRC. The HiPAT is an electromagnetic system (Penning-Malmberg design) consisting of a 4 Tesla superconductor, a high voltage confinement electrode system (operation up to 20 KV), and an ultra high vacuum test section (operating in the 10(exp -12) torr range). The system was designed to be portable with an ultimate goal of maintaining 10(exp 12) charged particles with a half-life of 18 days. Currently, this system is being experimentally evaluated using normal matter ions which are cheap to produce and relatively easy to handle. These normal ions provide a good indication of overall trap behavior, with the exception of assessing annihilation losses. The ions are produced external to HiPAT using two hydrogen ion sources, with adjustable beam energy and current. Ion are transported in a beam line and controlled through the use of electrostatic optics. These optics serve to both focus and gate the incoming ions, providing microsecond-timed pulses that are dynamically captured by cycling the HiPAT electric containment field like a 'trap door'. The layout of this system more closely simulates the operations expected at an actual antiproton production facility where 'packets' of antiprotons with pulse widths measured in

  17. 77 FR 72349 - SBI International, Inc. v. Mr. Howard Finkel c/o Cosco Container Lines; Notice of Filing of...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-12-05

    ... From the Federal Register Online via the Government Publishing Office FEDERAL MARITIME COMMISSION SBI International, Inc. v. Mr. Howard Finkel c/o Cosco Container Lines; Notice of Filing of Complaint...,'' against Mr. Howard Finkel c/o Cosco Container Lines, hereinafter ``Respondent.'' Complainant alleges...

  18. Flood-Inundation Maps for a 1.6-Mile Reach of Salt Creek, Wood Dale, Illinois

    USGS Publications Warehouse

    Soong, David T.; Murphy, Elizabeth A.; Sharpe, Jennifer B.

    2012-01-01

    Digital flood-inundation maps for a 1.6-mile reach of Salt Creek from upstream of the Chicago, Milwaukee, St. Paul & Pacific Railroad to Elizabeth Drive, Wood Dale, Illinois, were created by the U.S. Geological Survey (USGS) in cooperation with the DuPage County Stormwater Management Division. The inundation maps, which can be accessed through the USGS Flood Inundation Mapping Science Web site at http://water.usgs.gov/osw/flood_inundation/ depict estimates of the areal extent of flooding corresponding to selected water levels (gage heights) at the USGS streamgage on Salt Creek at Wood Dale, Illinois (station number 05531175). Current conditions at the USGS streamgage may be obtained on the Internet at http://waterdata.usgs.gov/usa/nwis/uv?05531175. In this study, flood profiles were computed for the stream reach by means of a one-dimensional unsteady flow Full EQuations (FEQ) model. The unsteady flow model was verified by comparing the rating curve output for a September 2008 flood event to discharge measurements collected at the Salt Creek at Wood Dale gage. The hydraulic model was then used to determine 14 water-surface profiles for gage heights at 0.5-ft intervals referenced to the streamgage datum and ranging from less than bankfull to approximately the highest recorded water level at the streamgage. The simulated water-surface profiles were then combined with a Geographic Information System (GIS) Digital Elevation Model (DEM) (derived from Light Detection and Ranging (LiDAR) data) in order to delineate the area flooded at each water level. The areal extent of the inundation was verified with high-water marks from a flood in July 2010 with a peak gage height of 14.08 ft recorded at the Salt Creek at Wood Dale gage. The availability of these maps along with Internet information regarding current gage height from USGS streamgages provide emergency management personnel and residents with information that is critical for flood response activities such as

  19. Construction and operation of the Howard T. Ricketts Laboratory.

    SciTech Connect

    Van Lonkhuyzen, R.; Stull, L.; Butler, J.; Chang, Y.; Allison, T.; O'Rourke, D.

    2006-01-01

    The National Institutes of Health (NIH) has proposed to partially fund the construction of the Howard T. Ricketts (HTR) regional biocontainment laboratory (RBL) by the University of Chicago at the U.S. Department of Energy's (DOE's) Argonne National Laboratory in Argonne, Illinois. The HTR Laboratory (HTRL) would be constructed, owned, and operated by the University of Chicago on land leased to it by DOE. The preferred project site is located north of Eastwood Drive and west of Outer Circle Road and is near the biological sciences building. This environmental assessment addresses the potential environmental effects resulting from construction and operation of the proposed facility. The proposed project involves the construction of a research facility with a footprint up to approximately 44,000 ft{sup 2} (4,088 m{sup 2}). The proposed building would house research laboratories, including Biosafety Level 2 and 3 biocontainment space, animal research facilities, administrative offices, and building support areas. The NIH has identified a need for new facilities to support research on potential bioterrorism agents and emerging and re-emerging infectious diseases, to protect the nation from such threats to public health. This research requires specialized laboratory facilities that are designed, managed, and operated to protect laboratory workers and the surrounding community from accidental exposure to agents. The proposed HTRL would provide needed biocontainment space to researchers and promote the advancement of knowledge in the disciplines of biodefense and emerging and re-emerging infectious diseases. Several alternatives were considered for the location of the proposed facility, as well as a no action alternative. The preferred alternative includes the construction of a research facility, up to 44,000 ft{sup 2} (4,088 m{sup 2}), at Argonne National Laboratory, a secure government location. Potential impacts to natural and cultural resources have been evaluated in

  20. Unpacking Pat Parker: Intersections and Revolutions in "Movement in Black".

    PubMed

    Washburn, Amy

    2015-01-01

    This article explores Pat Parker's poem "Movement in Black." It examines the ways in which she emblematizes intersectionality and simultaneity as forms of revolution in struggles of self and society. It begins with a theoretical and historical apparatus to contextualize Parker as an artist and activist. Then it offers a literary analysis of the poem, focusing on themes of time and space, marginalization and movement, difference and power, visibility and invisibility, and history and memory. It argues that Parker uses autobiographical writing to fuse personal and political sites of resistance.

  1. Unpacking Pat Parker: Intersections and Revolutions in "Movement in Black".

    PubMed

    Washburn, Amy

    2015-01-01

    This article explores Pat Parker's poem "Movement in Black." It examines the ways in which she emblematizes intersectionality and simultaneity as forms of revolution in struggles of self and society. It begins with a theoretical and historical apparatus to contextualize Parker as an artist and activist. Then it offers a literary analysis of the poem, focusing on themes of time and space, marginalization and movement, difference and power, visibility and invisibility, and history and memory. It argues that Parker uses autobiographical writing to fuse personal and political sites of resistance. PMID:26075685

  2. ARA67/PAT1 Functions as a Repressor To Suppress Androgen Receptor Transactivation

    PubMed Central

    Zhang, Yanqing; Yang, Yue; Yeh, Shuyuan; Chang, Chawnshang

    2004-01-01

    The androgen receptor (AR) may recruit multiple coregulators for proper or optimal transactivation. Here we report the identification and characterization of ARA67/PAT1 as an AR coregulator from a prostate cDNA library. ARA67/PAT1 was screened out as an AR N terminus interacting protein. Interaction mapping shows that the cooperation of multiple domains within ARA67/PAT1 may be required for the maximal interaction with AR. ARA67/PAT1 functions as a repressor with better suppressive effects on AR compared to glucocorticoid receptor and estrogen receptor. Further mechanism dissection reveals that the interrupted AR cytoplasmic-nuclear shuttling may play a major role in ARA67/PAT1 mediated suppression on AR. Together, these results suggest that ARA67/PAT1 may function as a novel repressor that can modulate AR function in prostate cancer. PMID:14729952

  3. Teaching Giants and Other Mentors of the Howard University: College of Medicine Class of 1952.

    PubMed

    Julian Haywood, L

    2015-03-01

    During the two decades following World War II, rapid changes occurred in medicine and in society as a whole in the United States including the establishment of the National Institutes of Health and the civil rights movement. This article profiles the teaching faculty of the Howard University College of Medicine that prepared the Class of 1952 for the decades that were to follow.

  4. The Pragmatic Idealist: Valerie Gross--Howard County Library, Columbia, MD

    ERIC Educational Resources Information Center

    Library Journal, 2004

    2004-01-01

    In the two years since Valerie Gross became director of the Howard County Library (HCL), it has won the county's Community Organization of the Year award, its Accessibility Award, and its Chamber of Commerce's Non-Profit Business of the Year ACE award for contributions to education, economic development, and quality of life. And for the first…

  5. Report of the Survey of Howard University: The Graduate School and Selected Ph.D. Programs.

    ERIC Educational Resources Information Center

    Office of Education (DHEW), Washington, DC.

    Results of the Office of Education's Bureau of Higher and Continuing Education's inspection of Howard University's Graduate School and the Ph.D. program are presented. The inspection was performed by conducting a survey of the administrative staff, the faculty, and student body, and by examining relevant facilities, laboratories, equipment,…

  6. Howard Community College Follow-Up of 1984 Entrants. Research Report Number 60.

    ERIC Educational Resources Information Center

    Seboda, Barbara L.

    In spring 1988, a follow-up study was conducted of students who first attended Howard Community College (HCC) in fall 1984. The primary objectives of the survey were to determine the students' educational and career achievements subsequent to attending HCC and to assess the effectiveness of the college from the students' perspective. All other…

  7. Commission on the Future of Howard Community College, Progress Report 2000.

    ERIC Educational Resources Information Center

    Howard Community Coll., Columbia, MD. Office of Planning and Evaluation.

    Presents the Commission on the Future of Howard Community College's (HCC) (Maryland) progress report 2000. Section 1, "Creating a World Class Learning Organization," presents strategic process and tactical action recommendations. Section 2, "Collaboration with Other Educational Organizations," discusses curricular and programmatic development,…

  8. Howard Community College 1986-87 Institutional Indicators for Proposed Operating Budget.

    ERIC Educational Resources Information Center

    Radcliffe, Susan; Novak, Virginia E.

    This report presents a series of graphs and tables, offering information on students, instruction, faculty, and finances at Howard Community College (HCC) in Columbia, Maryland. Part 1 provides profiles and projections of HCC's students, including a 10-year overview of full-time equivalent (FTE) enrollment, 1976-85; enrollment projections,…

  9. Highlights of the Survey of Employers of 1985 Howard Community College Graduates. Research Report Number 50.

    ERIC Educational Resources Information Center

    Livieratos, Barbara B.

    A study was conducted at Maryland's Howard Community College (HCC) to examine employers' views of the job readiness and vocational preparation of HCC graduates. The study sample included 55 employers of 1985 HCC graduates who agreed to have their employer contacted. The nursing program had the highest representation in the study sample, which…

  10. Academic Performance of Howard Community College Students in Transfer Institutions: Preliminary Findings. Research Report Number 37.

    ERIC Educational Resources Information Center

    Radcliffe, Susan K.

    A study was conducted at Howard Community College (HCC) to determine the performance of HCC students at transfer institutions. Four factors related to transfer success were examined: earning an associate degree at HCC; enrolling in a community college transfer program; length of time spent at HCC; and academic preparation and achievement at the…

  11. Howard Community College Follow-Up of 1987 Graduates. Research Report Number 61.

    ERIC Educational Resources Information Center

    Seboda, Barbara L.

    In 1988, a follow-up survey was conducted of 1987 Howard Community College (HCC) graduates to determine their employment and educational status, reasons for attending HCC, goal attainment, reasons for selecting HCC, ratings of the college, attendance pattern, transfer experiences, employment patterns, and satisfaction with the college and their…

  12. Howard Community College Report on the 2001 YESS Survey: Annual Survey of Student Satisfaction.

    ERIC Educational Resources Information Center

    Howard Community Coll., Columbia, MD. Office of Planning and Evaluation.

    This report details the results of the 2001 Yearly Evaluation of Services by Students Survey (YESS) from Howard Community College (HCC) (Maryland). The survey is given to randomly chosen class sections each spring. The number of students chosen to participate in the 2001 survey was 1,994, and the response rate was 38% (766 students). Ratings on…

  13. Commission on the Future of Howard Community College Progress Report, 2001.

    ERIC Educational Resources Information Center

    Howard Community Coll., Columbia, MD. Office of Planning and Evaluation.

    This Howard Community College (HCC) (Maryland) 2001 Progress Report makes assessments and recommendations in the following areas: (1) creating a world class learning organization; (2) collaboration with other educational organizations; (3) collaboration with business and industry; (4) economics and workforce development; (5) preparing students as…

  14. Distance Learners: Howard Community College's Fiscal Year 1989 Telecourse Students. Research Report Number 62.

    ERIC Educational Resources Information Center

    Livieratos, Barbara B.

    In fiscal year (FY) 1989, students enrolled in telecourses at Howard Community College (HCC) were surveyed at the end of each semester to determine their enrollment patterns, goals, sources of information about telecourses, reasons for enrolling in and satisfaction with the course, interest in computer-based distance learning, and personal…

  15. Howard Community College Follow-Up of 1988 Graduates. Research Report Number 66.

    ERIC Educational Resources Information Center

    Frank, Jean M.

    In 1989, a survey was conducted of all 266 students who had received an associate degree or certificate from Howard Community College (HCC) during fiscal year 1988. The purpose of the study was to evaluate programs and student services in the community colleges by collecting and analyzing data on the experiences and various outcomes of the…

  16. Howard Community College's 1992-1996 Transfer Graduates: A Trend Analysis.

    ERIC Educational Resources Information Center

    Frank, Jean

    This report aims to increase awareness regarding the characteristics, goals, and academic accomplishments of the students who graduated from Howard Community College (HCC) and transferred to four-year campuses. The data compiled in this report on transfer students were collected as part of follow-up surveys sent to all HCC graduates approximately…

  17. 75 FR 42814 - Central Railroad Company of Indianapolis-Abandonment Exemption-in Howard County, IN

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-07-22

    ... Surface Transportation Board Central Railroad Company of Indianapolis--Abandonment Exemption-- in Howard... CFR part 1152 subpart F--Exempt Abandonments to abandon 2.84 miles of rail line on CERA's Tipton... employee adversely affected by the abandonment shall be protected under Oregon Short Line Railroad &...

  18. The Evolution of Creativity, Giftedness, and Multiple Intelligences: An Interview with Ellen Winner and Howard Gardner

    ERIC Educational Resources Information Center

    Henshon, Suzanna E.

    2006-01-01

    This article presents an interview with Ellen Winner and Howard Gardner. Winner is Professor of Psychology at Boston College, and Senior Research Associate at Project Zero, Harvard Graduate School of Education. Her research focuses on learning and cognition in the arts in typical and gifted children. She is the author of over 100 articles and…

  19. Black, White, Greek...Like Who?: Howard University Student Perceptions of a White Fraternity on Campus

    ERIC Educational Resources Information Center

    Hughey, Matthew W.

    2006-01-01

    On March 2, 1867, the Historically Black College or University (HBCU) Howard University (HU) was founded in Washington, D. C. Almost exactly one year later, the all white fraternity of Pi Kappa Alpha was founded at the University of Virginia. Over 100 years later, on February 18 2006, fifty-five HU students became charter members of Pi Kappa Alpha…

  20. Project Probes Digital Media's Effect on Ethics: Howard Gardner Leads Team Studying Youths' Web Norms

    ERIC Educational Resources Information Center

    Viadero, Debra

    2008-01-01

    Noted Harvard psychologist Howard Gardner is leading a team studying the social and ethical norms of young people on the Web. Known as the "GoodPlay Project," the study is being financed with a grant from the Chicago-based Catherine T. MacArthur Foundation. What researchers hope to do through the project is fill a gap in the burgeoning research…

  1. Five Years of Right of Publicity: The Aftermath of "Zacchini v. Scripps-Howard."

    ERIC Educational Resources Information Center

    List, Karen

    The right of publicity is based on an individual's right to be free from the appropriation of his or her name or likeness by another for the other's financial benefit. As the courts' conception of the right of publicity was exapnding, so was the development of newsworthiness as a defense for media defendants. In "Zacchini v. Scripps-Howard," a…

  2. 75 FR 43138 - Ochoco National Forest, Lookout Mountain Ranger District; Oregon; Howard Elliot Johnson Fuels and...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-07-23

    ... Forest Service Ochoco National Forest, Lookout Mountain Ranger District; Oregon; Howard Elliot Johnson... Maurice Evans, Acting District Ranger, Lookout Mountain District, Ochoco National Forest, 3160 NE. Third.... SUPPLEMENTARY INFORMATION: Purpose and Need. The Lookout Mountain Ranger District has determined that there is...

  3. New Directions for Higher Education: Q&A with Muriel Howard on Public Higher Ed

    ERIC Educational Resources Information Center

    DiSalvio, Philip

    2014-01-01

    Nearly a year ago, "NEJHE" launched its "New Directions for Higher Education" series to examine emerging issues, trends, and ideas that have an impact on higher education policies, programs, and practices. In this installment, DiSalvio interviews Muriel A. Howard, president of the American Association of State Colleges and…

  4. Development of the Right of Publicity Since Zacchini v. Scripps-Howard.

    ERIC Educational Resources Information Center

    List, Karen K.

    This paper deals with the development of the right of publicity since "Zacchini v Scripps-Howard," a case in which the Supreme Court ruled in favor of Hugo Zacchini, a "human cannonball" who had charged that his professional property had been unlawfully appropriated by a television station that had filmed and shown his performance without…

  5. Revitalizing Politics Now and Then: Howard Zinn on Dissent, Democracy, and Education

    ERIC Educational Resources Information Center

    Cooley, Aaron

    2009-01-01

    This paper presents a discussion of Howard Zinn's intellectual and political ideas. Through the analysis of selections from his immense body of work, several interrelated themes emerge. Drawing more attention to these notions of dissent and democracy is crucial to revitalizing education at all levels and vital to advancing the public discourse…

  6. Principles and Practices Fostering Inclusive Excellence: Lessons from the Howard Hughes Medical Institute's Capstone Institutions

    ERIC Educational Resources Information Center

    DiBartolo, Patricia Marten; Gregg-Jolly, Leslie; Gross, Deborah; Manduca, Cathryn A.; Iverson, Ellen; Cooke, David B., III; Davis, Gregory K.; Davidson, Cameron; Hertz, Paul E.; Hibbard, Lisa; Ireland, Shubha K.; Mader, Catherine; Pai, Aditi; Raps, Shirley; Siwicki, Kathleen; Swartz, Jim E.

    2016-01-01

    Best-practices pedagogy in science, technology, engineering, and mathematics (STEM) aims for inclusive excellence that fosters student persistence. This paper describes principles of inclusivity across 11 primarily undergraduate institutions designated as Capstone Awardees in Howard Hughes Medical Institute's (HHMI) 2012 competition. The Capstones…

  7. Lost Voices of the Harlem Renaissance: Writing Assigned at Howard University, 1919-31.

    ERIC Educational Resources Information Center

    Zaluda, Scott

    1998-01-01

    Examines writing assignments, articles, textbooks, and other expressions of faculty thinking from courses about relationships among education, writing, and society in philosophy, English, history, and sociology at Howard University, a historically black university. Finds writing assignments at once conservative, subversive, and creative, in a…

  8. Written Assignments for Abnormal Psychology at Howard Community College, Fall 1988.

    ERIC Educational Resources Information Center

    Bell, James

    Designed for students enrolled in an Abnormal Psychology course at Howard Community College (Maryland), this booklet explains the requirements for the course's writing assignments, which are designed to teach the skills of comparison and contrast, analysis, critical thinking, and synthesis. Following an overview of class assignments and…

  9. 77 FR 29456 - Central Railroad Company of Indianapolis-Abandonment Exemption-in Howard County, IN

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-05-17

    ... Surface Transportation Board Central Railroad Company of Indianapolis--Abandonment Exemption-- in Howard... pending with the Surface Transportation Board (Board) or with any U.S. District Court or has been decided... public use conditions under 49 CFR 1152.28 must be filed by June 6, 2012, with the Surface...

  10. Critical Uses of History: A Memory of Howard Zinn and Video Journalism

    ERIC Educational Resources Information Center

    Price, Todd Alan

    2009-01-01

    In the following article I reflect on a few of the lessons provided by the late historian Howard Zinn, both his close reading of history, but even more so his critical insight "that history is made, not only received". Zinn used history much like a muckraking joumalist would: he wrote history not only "through" the eyes of common people, but "for"…

  11. 75 FR 44936 - Ochoco National Forest, Lookout Mountain Ranger District; Oregon; Howard Elliot Johnson Fuels and...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-07-30

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF AGRICULTURE Forest Service Ochoco National Forest, Lookout Mountain Ranger District; Oregon; Howard Elliot Johnson Fuels and Vegetation Management Project EIS Correction In notice document 2010-17803 beginning on...

  12. Perfluoroalkyl substances in the Daling River with concentrated fluorine industries in China: seasonal variation, mass flow, and risk assessment.

    PubMed

    Zhu, Zhaoyun; Wang, Tieyu; Meng, Jing; Wang, Pei; Li, Qifeng; Lu, Yonglong

    2015-07-01

    In the present study, we investigated concentrations and composition profiles of perfluoroalkyl substances (PFASs) in surface water from the Daling River in different seasons. The highest concentration of ΣPFASs (9540 ng L(-1)) and dominant homologues were found in surface water collected in summer. Perfluorobutanoic acid (PFBA), perfluorobutane sulfonate (PFBS), and perfluorooctanoic acid (PFOA) were the dominant PFASs in four seasons with a total contribution of over 90%. The discharge of two fluorine chemical industry parks was predicted to be the main contamination source of PFASs in the study area. The daily and annual mass flows were calculated according to data of detected PFAS concentrations and water discharge, and the annual mass loading of PFASs into the Bohai Sea from the Daling River reached to 461 kg year(-1). Hazard assessment indicated that concentrations of PFASs in study area had little risk to aquatic organisms and wildlife. However, the multiple health risk would be relatively high if the water was served as drinking water in the study area.

  13. The Efflux Pump Inhibitor Reserpine Selects Multidrug-Resistant Streptococcus pneumoniae Strains That Overexpress the ABC Transporters PatA and PatB▿ †

    PubMed Central

    Garvey, Mark I.; Piddock, Laura J. V.

    2008-01-01

    One way to combat multidrug-resistant microorganisms is the use of efflux pump inhibitors (EPIs). Spontaneous mutants resistant to the EPI reserpine selected from Streptococcus pneumoniae NCTC 7465 and R6 at a frequency suggestive of a single mutational event were also multidrug resistant. No mutations in pmrA (which encodes the efflux protein PmrA) were detected, and the expression of pmrA was unaltered in all mutants. In the reserpine-resistant multidrug-resistant mutants, the overexpression of both patA and patB, which encode ABC transporters, was associated with accumulation of low concentrations of antibiotics and dyes. The addition of sodium orthovanadate, an inhibitor of ABC efflux pumps, or the insertional inactivation of either gene restored wild-type antibiotic susceptibility and wild-type levels of accumulation. Only when patA was insertionally inactivated were both multidrug resistance and reserpine resistance lost. Strains in which patA was insertionally inactivated grew significantly more slowly than the wild type. These data indicate that the overexpression of both patA and patB confers multidrug resistance in S. pneumoniae but that only patA is involved in reserpine resistance. The selection of reserpine-resistant multidrug-resistant pneumococci has implications for analogous systems in other bacteria or in cancer. PMID:18362193

  14. Thermodynamics and Chemistry by Howard DeVoe

    NASA Astrophysics Data System (ADS)

    Gislason, Eric A.

    2001-09-01

    Prentice Hall: Upper Saddle River, NJ, 2001. 439 pp. ISBN 0-02-328741-1. $90.00. Thermodynamics is a deceptively difficult subject that few people master in their first exposure (typically in the junior-level physical chemistry course). Because of this, a clear, well-written textbook is always welcome to help students and teachers master this material. Such a book is Thermodynamics and Chemistry, by Howard DeVoe of the University of Maryland. This book is written as a one-semester textbook for senior undergraduates and graduate students who have had a previous course on the topic. This is a long book (400 pages of text, 25 pages of appendices), and it is unlikely that an instructor can cover everything in one semester. On the other hand this length does allow the author to cover topics such as a "liquid solution in a centrifugal field" that shorter books would omit. All important topics in chemical thermodynamics are covered. After two introductory chapters, the three laws of thermodynamics are taken up in Chapters 3 and 4. Pure substances, including phase transitions, are treated in Chapters 5 and 6. Chapter 7, "Mixtures," is a long chapter that covers, among other topics, partial molar quantities, activities, and activity coefficients. Chemical reactions are treated in Chapter 8 and there is a detailed discussion of equilibrium in various multicomponent systems in Chapter 9. The book is completed with a discussion of the phase rule (Chapter 10) and a brief chapter on galvanic cells. There are a number of things I like about this book. First, DeVoe takes great care in defining important thermodynamic words such as the thermodynamic state of a system. Similarly, he makes the distinction between process and path understandable, and this allows him to clearly define a reversible process as well as spontaneous and impossible processes. Section 4.1 then contains the sentence "An irreversible process is a spontaneous process whose reverse is an impossible process." This

  15. Thermodynamics and Chemistry by Howard DeVoe

    NASA Astrophysics Data System (ADS)

    Gislason, Eric A.

    2001-09-01

    Prentice Hall: Upper Saddle River, NJ, 2001. 439 pp. ISBN 0-02-328741-1. $90.00. Thermodynamics is a deceptively difficult subject that few people master in their first exposure (typically in the junior-level physical chemistry course). Because of this, a clear, well-written textbook is always welcome to help students and teachers master this material. Such a book is Thermodynamics and Chemistry, by Howard DeVoe of the University of Maryland. This book is written as a one-semester textbook for senior undergraduates and graduate students who have had a previous course on the topic. This is a long book (400 pages of text, 25 pages of appendices), and it is unlikely that an instructor can cover everything in one semester. On the other hand this length does allow the author to cover topics such as a "liquid solution in a centrifugal field" that shorter books would omit. All important topics in chemical thermodynamics are covered. After two introductory chapters, the three laws of thermodynamics are taken up in Chapters 3 and 4. Pure substances, including phase transitions, are treated in Chapters 5 and 6. Chapter 7, "Mixtures," is a long chapter that covers, among other topics, partial molar quantities, activities, and activity coefficients. Chemical reactions are treated in Chapter 8 and there is a detailed discussion of equilibrium in various multicomponent systems in Chapter 9. The book is completed with a discussion of the phase rule (Chapter 10) and a brief chapter on galvanic cells. There are a number of things I like about this book. First, DeVoe takes great care in defining important thermodynamic words such as the thermodynamic state of a system. Similarly, he makes the distinction between process and path understandable, and this allows him to clearly define a reversible process as well as spontaneous and impossible processes. Section 4.1 then contains the sentence "An irreversible process is a spontaneous process whose reverse is an impossible process." This

  16. Public Speaking on the Streets and in the Ivory Tower: A Comparison of the Dale Carnegie Course and the Academic Course.

    ERIC Educational Resources Information Center

    Miller, Christine M.

    Many speech communication educators and researchers look upon Dale Carnegie's public speaking course with derision for its methods as well as its motives. A comparison of Carnegie's course with university courses in speech communication reveals a number of differences between the two, which in part explains this attitude. Carnegie began his course…

  17. Rationale, Design, and Methods of the Preschool ADHD Treatment Study (PATS)

    ERIC Educational Resources Information Center

    Kollins, Scott; Greenhill, Laurence; Swanson, James; Wigal, Sharon; Abikoff, Howard; McCracken, James; Riddle, Mark; McGough, James; Vitiello, Benedetto; Wigal, Tim; Skrobala, Anne; Posner, Kelly; Ghuman, Jaswinder; Davies, Mark; Cunningham, Charles; Bauzo, Audrey

    2006-01-01

    Objective: To describe the rationale and design of the Preschool ADHD Treatment Study (PATS). Method: PATS was a National Institutes of Mental Health-funded, multicenter, randomized, efficacy trial designed to evaluate the short-term (5 weeks) efficacy and long-term (40 weeks) safety of methylphenidate (MPH) in preschoolers with…

  18. A Training Program for Pat Frisking Female Inmates for New York City Department of Correction Officers.

    ERIC Educational Resources Information Center

    Andrews, Brenda M.

    A training program was developed for male correction officers confronted with pat frisking female inmates. The training goal was to have correction officers in women's prisons identify female inmates' contraband and be able to determine alternatives for male correction officers who were confronted with pat frisking female inmates. The Dick and…

  19. The Preschool Attention-Deficit/Hyperactivity Disorder Treatment Study (PATS) 6-Year Follow-Up

    ERIC Educational Resources Information Center

    Riddle, Mark A.; Yershova, Kseniya; Lazzaretto, Deborah; Paykina, Natalya; Yenokyan, Gayane; Greenhill, Laurence; Abikoff, Howard; Vitiello, Benedetto; Wigal, Tim; McCracken, James T.; Kollins, Scott H.; Murray, Desiree W.; Wigal, Sharon; Kastelic, Elizabeth; McGough, James J.; dosReis, Susan; Bauzo-Rosario, Audrey; Stehli, Annamarie; Posner, Kelly

    2013-01-01

    Objective: To describe the clinical course of attention-deficit/hyperactivity disorder (ADHD) symptom severity and diagnosis from ages 3 to 5 up to 9 to 12 years during a 6-year follow-up after the original Preschool ADHD Treatment Study (PATS). Method: A total of 207 participants (75% male) from the original PATS, assessed at baseline (mean age,…

  20. Sir Howard Walter Florey--the force behind the development of penicillin.

    PubMed

    Ligon, B Lee

    2004-04-01

    The development of penicillin was a watershed in the battle against infectious diseases. The primary individuals responsible for its discovery and development were Sir Alexander Fleming, Sir Howard Walter Florey, and Ernst B. Chain, now primary figures in the annals of medical history. The individual who serendipitously "discovered" penicillin was Sir Alexander Fleming. Despite the determination displayed by Fleming, little notice was given to his discovery for more than a decade, and the active substance was not isolated. Finally, in 1939, Florey, along with Chain, led a team of British scientists who successfully manufactured the drug from the liquid broth in which penicillin grows. They, along with Fleming, were given the 1945 Nobel Prize in Physiology or Medicine for their roles in the discovery and development of this agent. This biography focuses on the life and work of Sir Howard Walter Florey.

  1. Molecular cloning and characterization of oocyte-specific Pat1a in Rana rugosa frogs.

    PubMed

    Nakamura, Yoriko; Iwasaki, Takehiro; Umei, Yosuke; Saotome, Kazuhiro; Nakajima, Yukiko; Kitahara, Shoichi; Uno, Yoshinobu; Matsuda, Yoichi; Oike, Akira; Kodama, Maho; Nakamura, Masahisa

    2015-10-01

    The Pat1 gene is expressed in the immature oocytes of Xenopus, and is reportedly involved in regulating the translation of maternal mRNAs required for oocyte-maturation. However, it is still unknown when Pat1a first appears in the differentiating ovary of amphibians. To address this issue, we isolated the full-length Pat1a cDNA from the frog Rana rugosa and examined its expression in the differentiating ovary of this frog. Among eight different tissues examined, the Pat1a mRNA was detectable in only the ovary. When frozen sections from the ovaries of tadpoles at various stages of development were immunostained for Vasa-a germ cell-specific protein-and Pat1a, Vasa-immunopositive signals were observed in all of the germ cells, whereas Pat1a signals were confined to the growing oocytes (50-200 μm in diameter), and absent from small germ cells (<50 μm in diameter). Forty days after testosterone injection into tadpoles to induce female-to-male sex-reversal, Pat1a-immunoreactive oocytes had disappeared completely from the sex-reversed gonad, but Vasa-positive small germ cells persisted. Thus, Pat1a would be a good marker for identifying the sexual status of the sex-reversing gonad in amphibians. In addition, fluorescence in situ hybridization analysis showed Pat1a to have an autosomal locus, suggesting that Pat1a transcription is probably regulated by a tissue-specific transcription factor in R. rugosa.

  2. Dale Reed with X-38 and a Subscale Model Used in Test Program

    NASA Technical Reports Server (NTRS)

    1997-01-01

    Dale Reed, a NASA engineer who worked on the original lifting-body research programs in the 1960s and 1970s, stands with a scale-model X-38 that was used in 1995 research flights, with a full-scale X-38 (80 percent of the size of a potential Crew Return Vehicle) behind him. The X-38 Crew Return Vehicle (CRV) research project is designed to develop the technology for a prototype emergency crew return vehicle, or lifeboat, for the International Space Station. The project is also intended to develop a crew return vehicle design that could be modified for other uses, such as a joint U.S. and international human spacecraft that could be launched on the French Ariane-5 Booster. The X-38 project is using available technology and off-the-shelf equipment to significantly decrease development costs. Original estimates to develop a capsule-type crew return vehicle were estimated at more than $2 billion. X-38 project officials have estimated that development costs for the X-38 concept will be approximately one quarter of the original estimate. Off-the-shelf technology is not necessarily 'old' technology. Many of the technologies being used in the X-38 project have never before been applied to a human-flight spacecraft. For example, the X-38 flight computer is commercial equipment currently used in aircraft and the flight software operating system is a commercial system already in use in many aerospace applications. The video equipment for the X-38 is existing equipment, some of which has already flown on the space shuttle for previous NASA experiments. The X-38's primary navigational equipment, the Inertial Navigation System/Global Positioning System, is a unit already in use on Navy fighters. The X-38 electromechanical actuators come from previous joint NASA, U.S. Air Force, and U.S. Navy research and development projects. Finally, an existing special coating developed by NASA will be used on the X-38 thermal tiles to make them more durable than those used on the space

  3. Short-Term Memory after All: Comment on Sederberg, Howard, and Kahana (2008)

    ERIC Educational Resources Information Center

    Usher, Marius; Davelaar, Eddy J.; Haarmann, Henk J.; Goshen-Gottstein, Yonatan

    2008-01-01

    P. B. Sederberg, M. W. Howard, and M. J. Kahana have proposed an updated version of the temporal-context model (TCM-A). In doing so, they accepted the challenge of developing a single-store model to account for the dissociations between short- and long-term recency effects that were reviewed by E. J. Davelaar, Y. Goshen-Gottstein, A. Ashkenazi, H.…

  4. Teaching Giants and Other Mentors of the Howard University: College of Medicine Class of 1952.

    PubMed

    Julian Haywood, L

    2015-03-01

    During the two decades following World War II, rapid changes occurred in medicine and in society as a whole in the United States including the establishment of the National Institutes of Health and the civil rights movement. This article profiles the teaching faculty of the Howard University College of Medicine that prepared the Class of 1952 for the decades that were to follow. PMID:27282727

  5. Special Article: Howard Dittrick: curator to the McMechans' legacy journal.

    PubMed

    Bause, George S; Edmonson, James M

    2012-12-01

    A noted medical historian and museum curator, Canadian American Howard Dittrick was a Cleveland gynecologist who served as Directing Editor of Current Researches in Anesthesia and Analgesia (1940-1954). In the aftermath of World War II, even after Congresses of Anesthetists had resumed, Dittrick and his editorial board allowed their yellow, then tan-covered journal, the so-called "yellow peril," to languish into near irrelevance.

  6. From rat pathophysiology to genomic medicine: an interview with Howard Jacob

    PubMed Central

    2016-01-01

    Howard Jacob is best known for pioneering genomic sequencing of a patient to solve a mysterious pediatric case in 2010. With roots in pharmacology and cardiovascular disease, however, his career has largely been dedicated to dissecting the physiology and genetics of the rat to help understand complex human diseases. Howard was Director of the Human and Molecular Genetics Center at the Medical College of Wisconsin for 16 years, during which time he applied a combination of approaches, including quantitative genetics, integrative physiology and next-generation sequencing, in rat models to shed light on cardiovascular, metabolic and renal disorders. He was a key contributor to the genomic toolbox for rat research, and generated the first targeted-knockout rat models using zinc-finger-nuclease technology. He also contributed to sequencing of the rat genome and establishment of the Rat Genome Database. In this interview, Howard provides his perspectives on the past, present and future of rat-based translational research and explains why, despite his many successes as the leader of a rat group, he recently made the transition to clinical genomics. PMID:27736743

  7. Issues in development, evaluation, and use of the NASA Preflight Adaptation Trainer (PAT)

    NASA Technical Reports Server (NTRS)

    Lane, Norman E.; Kennedy, Robert S.

    1988-01-01

    The Preflight Adaptation Trainer (PAT) is intended to reduce or alleviate space adaptation syndrome by providing opportunities for portions of that adaptation to occur under normal gravity conditions prior to space flight. Since the adaptation aspects of the PAT objectives involve modification not only of the behavior of the trainee, but also of sensiomotor skills which underly the behavioral generation, the defining of training objectives of the PAT utilizes four mechanisms: familiarization, demonstration, training and adaptation. These mechanisms serve as structural reference points for evaluation, drive the content and organization of the training procedures, and help to define the roles of the PAT instructors and operators. It was determined that three psychomotor properties are most critical for PAT evaluation: reliability; sensitivity; and relevance. It is cause for concern that the number of measures available to examine PAT effects exceed those that can be properly studied with the available sample sizes; special attention will be required in selection of the candidate measure set. The issues in PAT use and application within a training system context are addressed through linking the three training related mechanisms of familiarization, demonstration and training to the fourth mechanism, adaptation.

  8. Structural insights into HetR-PatS interaction involved in cyanobacterial pattern formation.

    PubMed

    Hu, Hai-Xi; Jiang, Yong-Liang; Zhao, Meng-Xi; Cai, Kun; Liu, Sanling; Wen, Bin; Lv, Pei; Zhang, Yonghui; Peng, Junhui; Zhong, Hui; Yu, Hong-Mei; Ren, Yan-Min; Zhang, Zhiyong; Tian, Changlin; Wu, Qingfa; Oliveberg, Mikael; Zhang, Cheng-Cai; Chen, Yuxing; Zhou, Cong-Zhao

    2015-01-01

    The one-dimensional pattern of heterocyst in the model cyanobacterium Anabaena sp. PCC 7120 is coordinated by the transcription factor HetR and PatS peptide. Here we report the complex structures of HetR binding to DNA, and its hood domain (HetRHood) binding to a PatS-derived hexapeptide (PatS6) at 2.80 and 2.10 Å, respectively. The intertwined HetR dimer possesses a couple of novel HTH motifs, each of which consists of two canonical α-helices in the DNA-binding domain and an auxiliary α-helix from the flap domain of the neighboring subunit. Two PatS6 peptides bind to the lateral clefts of HetRHood, and trigger significant conformational changes of the flap domain, resulting in dissociation of the auxiliary α-helix and eventually release of HetR from the DNA major grove. These findings provide the structural insights into a prokaryotic example of Turing model. PMID:26576507

  9. Structural insights into HetR−PatS interaction involved in cyanobacterial pattern formation

    PubMed Central

    Hu, Hai-Xi; Jiang, Yong-Liang; Zhao, Meng-Xi; Cai, Kun; Liu, Sanling; Wen, Bin; Lv, Pei; Zhang, Yonghui; Peng, Junhui; Zhong, Hui; Yu, Hong-Mei; Ren, Yan-Min; Zhang, Zhiyong; Tian, Changlin; Wu, Qingfa; Oliveberg, Mikael; Zhang, Cheng-Cai; Chen, Yuxing; Zhou, Cong-Zhao

    2015-01-01

    The one-dimensional pattern of heterocyst in the model cyanobacterium Anabaena sp. PCC 7120 is coordinated by the transcription factor HetR and PatS peptide. Here we report the complex structures of HetR binding to DNA, and its hood domain (HetRHood) binding to a PatS-derived hexapeptide (PatS6) at 2.80 and 2.10 Å, respectively. The intertwined HetR dimer possesses a couple of novel HTH motifs, each of which consists of two canonical α-helices in the DNA-binding domain and an auxiliary α-helix from the flap domain of the neighboring subunit. Two PatS6 peptides bind to the lateral clefts of HetRHood, and trigger significant conformational changes of the flap domain, resulting in dissociation of the auxiliary α-helix and eventually release of HetR from the DNA major grove. These findings provide the structural insights into a prokaryotic example of Turing model. PMID:26576507

  10. PAT4 is abundantly expressed in excitatory and inhibitory neurons as well as epithelial cells.

    PubMed

    Roshanbin, Sahar; Hellsten, Sofie V; Tafreshiha, Atieh; Zhu, Yinan; Raine, Amanda; Fredriksson, Robert

    2014-04-01

    PAT4, the fourth member of the SLC36/proton dependent amino acid transporter (PAT) family, is a high-affinity, low capacity electroneutral transporter of neutral amino acids like proline and tryptophan. It has also been associated with the function of mTORC1, a complex in the mammalian target of rapamycin (mTOR) pathway. We performed in situ hybridization and immunohistological analysis to determine the expression profile of PAT4, as well as an RT-PCR study on tissue from mice exposed to leucine. We performed a phylogenetic analysis to determine the evolutionary origin of PAT4. The in situ hybridization and the immunohistochemistry on mouse brain sections and hypothalamic cells showed abundant PAT4 expression in the mouse brain intracellularly in both inhibitory and excitatory neurons, partially co-localizing with lysosomal markers and epithelial cells lining the ventricles. Its location in epithelial cells around the ventricles indicates a transport of substrates across the blood brain barrier. Phylogenetic analysis showed that PAT4 belongs to an evolutionary old family most likely predating animals, and PAT4 is the oldest member of that family. PMID:24530433

  11. Polyamine metabolism is altered in unpollinated parthenocarpic pat-2 tomato ovaries.

    PubMed

    Fos, Mariano; Proaño, Karina; Alabadí, David; Nuez, Fernando; Carbonell, Juan; García-Martínez, José L

    2003-01-01

    Facultative parthenocarpy induced by the recessive mutation pat-2 in tomato (Lycopersicon esculentum Mill.) depends on gibberellins (GAs) and is associated with changes in GA content in unpollinated ovaries. Polyamines (PAs) have also been proposed to play a role in early tomato fruit development. We therefore investigated whether PAs are able to induce parthenocarpy and whether the pat-2 mutation alters the content and metabolism of PAs in unpollinated ovaries. Application of putrescine, spermidine, and spermine to wild-type unpollinated tomato ovaries (cv Madrigal [MA/wt]) induced partial parthenocarpy. Parthenocarpic growth of MA/pat-2 (a parthenocarpic near-isogenic line to MA/wt) ovaries was negated by paclobutrazol (GA biosynthesis inhibitor), and this inhibition was counteracted by spermidine. Application of alpha-difluoromethyl-ornithine (-Orn) and/or alpha-difluoromethyl-arginine (-Arg), irreversible inhibitors of the putrescine biosynthesis enzymes Orn decarboxylase (ODC) and Arg decarboxylase, respectively, prevented growth of unpollinated MA/pat-2 ovaries. Alpha-difluoromethyl-Arg inhibition was counteracted by putrescine and GA(3), whereas that of alpha-difluoromethyl-Orn was counteracted by GA(3) but not by putrescine or spermidine. In unpollinated MA/pat-2 ovaries, the content of free spermine was significantly higher than in MA/wt ovaries. ODC activity was higher in pat-2 ovaries than in MA/wt. Transcript levels of genes encoding ODC and spermidine synthase were also higher in MA/pat-2. All together, these results strongly suggest that the parthenocarpic ability of pat-2 mutants depends on elevated PAs levels in unpollinated mutant ovaries, which correlate with an activation of the ODC pathway, probably as a consequence of elevated GA content in unpollinated pat-2 tomato ovaries. PMID:12529543

  12. Polyamine metabolism is altered in unpollinated parthenocarpic pat-2 tomato ovaries.

    PubMed

    Fos, Mariano; Proaño, Karina; Alabadí, David; Nuez, Fernando; Carbonell, Juan; García-Martínez, José L

    2003-01-01

    Facultative parthenocarpy induced by the recessive mutation pat-2 in tomato (Lycopersicon esculentum Mill.) depends on gibberellins (GAs) and is associated with changes in GA content in unpollinated ovaries. Polyamines (PAs) have also been proposed to play a role in early tomato fruit development. We therefore investigated whether PAs are able to induce parthenocarpy and whether the pat-2 mutation alters the content and metabolism of PAs in unpollinated ovaries. Application of putrescine, spermidine, and spermine to wild-type unpollinated tomato ovaries (cv Madrigal [MA/wt]) induced partial parthenocarpy. Parthenocarpic growth of MA/pat-2 (a parthenocarpic near-isogenic line to MA/wt) ovaries was negated by paclobutrazol (GA biosynthesis inhibitor), and this inhibition was counteracted by spermidine. Application of alpha-difluoromethyl-ornithine (-Orn) and/or alpha-difluoromethyl-arginine (-Arg), irreversible inhibitors of the putrescine biosynthesis enzymes Orn decarboxylase (ODC) and Arg decarboxylase, respectively, prevented growth of unpollinated MA/pat-2 ovaries. Alpha-difluoromethyl-Arg inhibition was counteracted by putrescine and GA(3), whereas that of alpha-difluoromethyl-Orn was counteracted by GA(3) but not by putrescine or spermidine. In unpollinated MA/pat-2 ovaries, the content of free spermine was significantly higher than in MA/wt ovaries. ODC activity was higher in pat-2 ovaries than in MA/wt. Transcript levels of genes encoding ODC and spermidine synthase were also higher in MA/pat-2. All together, these results strongly suggest that the parthenocarpic ability of pat-2 mutants depends on elevated PAs levels in unpollinated mutant ovaries, which correlate with an activation of the ODC pathway, probably as a consequence of elevated GA content in unpollinated pat-2 tomato ovaries.

  13. Polyamine Metabolism Is Altered in Unpollinated Parthenocarpic pat-2 Tomato Ovaries1

    PubMed Central

    Fos, Mariano; Proaño, Karina; Alabadí, David; Nuez, Fernando; Carbonell, Juan; García-Martínez, José L.

    2003-01-01

    Facultative parthenocarpy induced by the recessive mutation pat-2 in tomato (Lycopersicon esculentum Mill.) depends on gibberellins (GAs) and is associated with changes in GA content in unpollinated ovaries. Polyamines (PAs) have also been proposed to play a role in early tomato fruit development. We therefore investigated whether PAs are able to induce parthenocarpy and whether the pat-2 mutation alters the content and metabolism of PAs in unpollinated ovaries. Application of putrescine, spermidine, and spermine to wild-type unpollinated tomato ovaries (cv Madrigal [MA/wt]) induced partial parthenocarpy. Parthenocarpic growth of MA/pat-2 (a parthenocarpic near-isogenic line to MA/wt) ovaries was negated by paclobutrazol (GA biosynthesis inhibitor), and this inhibition was counteracted by spermidine. Application of α-difluoromethyl-ornithine (-Orn) and/or α-difluoromethyl-arginine (-Arg), irreversible inhibitors of the putrescine biosynthesis enzymes Orn decarboxylase (ODC) and Arg decarboxylase, respectively, prevented growth of unpollinated MA/pat-2 ovaries. α-Difluoromethyl-Arg inhibition was counteracted by putrescine and GA3, whereas that of α-difluoromethyl-Orn was counteracted by GA3 but not by putrescine or spermidine. In unpollinated MA/pat-2 ovaries, the content of free spermine was significantly higher than in MA/wt ovaries. ODC activity was higher in pat-2 ovaries than in MA/wt. Transcript levels of genes encoding ODC and spermidine synthase were also higher in MA/pat-2. All together, these results strongly suggest that the parthenocarpic ability of pat-2 mutants depends on elevated PAs levels in unpollinated mutant ovaries, which correlate with an activation of the ODC pathway, probably as a consequence of elevated GA content in unpollinated pat-2 tomato ovaries. PMID:12529543

  14. Pointing, acquisition and tracking (PAT) subsystems and components for optical space communication systems

    NASA Astrophysics Data System (ADS)

    Kern, R. H.; Kugel, U.

    1989-10-01

    The pointing, acquisition, and tracking (PAT) subsystem that will be incorporated by optical space communications transceivers must furnish very precise beam steering due to the highly collimated nature of the laser beams employed. The PAT subsystem must also be able to cover an angular rate of more than a full hemisphere, if it is intended for use on a LEO satellite which communicates with another in GEO. The present PAT is composed of a coarse-pointing assembly, a fine-pointing assembly, and a point-ahead assembly; their interactive operation is managed by a control law electronics unit. A CO2 laser is assumed to be the transmitter.

  15. Oracle Applications Patch Administration Tool (PAT) Beta Version

    2002-01-04

    PAT is a Patch Administration Tool that provides analysis, tracking, and management of Oracle Application patches. This includes capabilities as outlined below: Patch Analysis & Management Tool Outline of capabilities: Administration Patch Data Maintenance -- track Oracle Application patches applied to what database instance & machine Patch Analysis capture text files (readme.txt and driver files) form comparison detail report comparison detail PL/SQL package comparison detail SQL scripts detail JSP module comparison detail Parse and loadmore » the current applptch.txt (10.7) or load patch data from Oracle Application database patch tables (11i) Display Analysis -- Compare patch to be applied with current Oracle Application installed Appl_top code versions Patch Detail Module comparison detail Analyze and display one Oracle Application module patch. Patch Management -- automatic queue and execution of patches Administration Parameter maintenance -- setting for directory structure of Oracle Application appl_top Validation data maintenance -- machine names and instances to patch Operation Patch Data Maintenance Schedule a patch (queue for later execution) Run a patch (queue for immediate execution) Review the patch logs Patch Management Reports« less

  16. Oracle Applications Patch Administration Tool (PAT) Beta Version

    SciTech Connect

    2002-01-04

    PAT is a Patch Administration Tool that provides analysis, tracking, and management of Oracle Application patches. This includes capabilities as outlined below: Patch Analysis & Management Tool Outline of capabilities: Administration Patch Data Maintenance -- track Oracle Application patches applied to what database instance & machine Patch Analysis capture text files (readme.txt and driver files) form comparison detail report comparison detail PL/SQL package comparison detail SQL scripts detail JSP module comparison detail Parse and load the current applptch.txt (10.7) or load patch data from Oracle Application database patch tables (11i) Display Analysis -- Compare patch to be applied with current Oracle Application installed Appl_top code versions Patch Detail Module comparison detail Analyze and display one Oracle Application module patch. Patch Management -- automatic queue and execution of patches Administration Parameter maintenance -- setting for directory structure of Oracle Application appl_top Validation data maintenance -- machine names and instances to patch Operation Patch Data Maintenance Schedule a patch (queue for later execution) Run a patch (queue for immediate execution) Review the patch logs Patch Management Reports

  17. Solid state laser communications in space (SOLACOS) position, acquisition, and tracking (PAT) subsystem implementation

    NASA Astrophysics Data System (ADS)

    Flemmig, Joerg; Pribil, Klaus

    1994-09-01

    This paper presents the concept and implementation aspects of the Pointing, Acquisition and Tracking Subsystem (PAT) which is developed in the frame of the SOLACOS (Solid State Laser Communications in Space) program.

  18. Air transport of plutonium metal : content expansion initiative for the Plutonium Air Transportable (PAT-1) packaging.

    SciTech Connect

    Mann, Paul T.; Caviness, Michael L.; Yoshimura, Richard Hiroyuki

    2010-06-01

    The National Nuclear Security Administration (NNSA) has submitted an application to the Nuclear Regulatory Commission (NRC) for the air shipment of plutonium metal within the Plutonium Air Transportable (PAT-1) packaging. The PAT-1 packaging is currently authorized for the air transport of plutonium oxide in solid form only. The INMM presentation will provide a limited overview of the scope of the plutonium metal initiative and provide a status of the NNSA application to the NRC.

  19. Air transport of plutonium metal: content expansion initiative for the plutonium air transportable (PAT01) packaging

    SciTech Connect

    Caviness, Michael L; Mann, Paul T

    2010-01-01

    The National Nuclear Security Administration (NNSA) has submitted an application to the Nuclear Regulatory Commission (NRC) for the air shipment of plutonium metal within the Plutonium Air Transportable (PAT-1) packaging. The PAT-1 packaging is currently authorized for the air transport of plutonium oxide in solid form only. The INMM presentation will provide a limited overview of the scope of the plutonium metal initiative and provide a status of the NNSA application to the NRC.

  20. GeoPAT: A toolbox for pattern-based information retrieval from large geospatial databases

    NASA Astrophysics Data System (ADS)

    Jasiewicz, Jarosław; Netzel, Paweł; Stepinski, Tomasz

    2015-07-01

    Geospatial Pattern Analysis Toolbox (GeoPAT) is a collection of GRASS GIS modules for carrying out pattern-based geospatial analysis of images and other spatial datasets. The need for pattern-based analysis arises when images/rasters contain rich spatial information either because of their very high resolution or their very large spatial extent. Elementary units of pattern-based analysis are scenes - patches of surface consisting of a complex arrangement of individual pixels (patterns). GeoPAT modules implement popular GIS algorithms, such as query, overlay, and segmentation, to operate on the grid of scenes. To achieve these capabilities GeoPAT includes a library of scene signatures - compact numerical descriptors of patterns, and a library of distance functions - providing numerical means of assessing dissimilarity between scenes. Ancillary GeoPAT modules use these functions to construct a grid of scenes or to assign signatures to individual scenes having regular or irregular geometries. Thus GeoPAT combines knowledge retrieval from patterns with mapping tasks within a single integrated GIS environment. GeoPAT is designed to identify and analyze complex, highly generalized classes in spatial datasets. Examples include distinguishing between different styles of urban settlements using VHR images, delineating different landscape types in land cover maps, and mapping physiographic units from DEM. The concept of pattern-based spatial analysis is explained and the roles of all modules and functions are described. A case study example pertaining to delineation of landscape types in a subregion of NLCD is given. Performance evaluation is included to highlight GeoPAT's applicability to very large datasets. The GeoPAT toolbox is available for download from

  1. [The materialistic strategy of Henry Howard, a Québec alienist (1815-1887).].

    PubMed

    Fugère, A

    1978-01-01

    At the end of the 19th century, Henry Howard appeared as the first Quebec "aliéniste" to attempt to develop an inclusive theoretical treatise on the question of madness. Why this sudden upheaval ? There existed numerous european theories ; that might have sufficed. The author attempts to show the multiple connections of this theoretical pro-lect to the socio-political problems of québécois society. Howard tries, from the perspective of the theory which he elaborates, to intervene in the major discussions affecting Quebec: ultra montanism, the problem of universal compusory education, public morality. At a more professional level he attempts to intervene in the legal dispute opposing judicial and medical competence in the cases of insanity pleas, as well as in the question of the scientific and social status of the "aliéniste". The involvement in these struggles manifests itself in Howard's treatise as much at the level of the concepts themselves, and their architecture, as at the thematic level and in the applications which he naturally draws. It is not onty external and contingent but works on the text from the inside as well. The article attempts to suggest that at the very moment that the treatise on the alienated-which would become the "science" that we know today- seeks to structure itself into a theory, it cannot break its attachment to its determining social context and that, ignoring its ideological role it, in truth, succeeds only in creating illusion and the impression of a progressive process, all the while being circumstantial and having a verted interest.

  2. Committee on the forfeiture of assets in criminal offences of the Howard League of Penal Reform.

    PubMed

    Nicol, A

    1983-01-01

    The profits of illicit drug trafficking have attracted world-wide attention. In 1980, a decision of the House of Lords in the United Kingdom ruled that the English criminal courts had no power to forfeit such profits even though earned by convicted defendants. This lacuna in the law aroused considerable criticism and the Howard League of Penal Reform established an independent committee to propose reforms. Its report will be published shortly. This article discusses some of the issues which the Committee faced, particularly the delicate balance between the interest of the State in ensuring that crime does not pay and a commitment to due process before financial punishments are imposed.

  3. The efficacy of Watch PAT in obstructive sleep apnea syndrome diagnosis.

    PubMed

    Körkuyu, Emine; Düzlü, Mehmet; Karamert, Recep; Tutar, Hakan; Yılmaz, Metin; Çiftçi, Bülent; Güven, Selma Fırat

    2015-01-01

    Polysomnography is currently considered as the gold standard for the diagnosis of obstructive sleep apnea syndrome (OSAS). But high expense and the backlog of the sleep centers have resulted in a search for an alternative method of diagnosis. The aim of this study is to assess the efficacy and reliability of Watch PAT as an alternate option in OSAS diagnosis. The patients have worn a Watch PAT(®) 200 device in the sleep laboratory during a standard polysomnography. The correlation in REM and Non-REM AHI scores, sleep periods and the mean O2 saturation percentage between Watch PAT and PSG sleep studies were assessed. There was a statistically significant very strong correlation between PSG and Watch PAT AHI scores (Spearman's rho = 0.802 p < 0.001). The mean recording time with PSG and Watch PAT was 463.06 ± 37.08 and 469.33 ± 72.81 min, respectively, and there was no statistically significant difference (p = 0.068). However, there was a statistically significant difference between two methods regarding the average sleep time and REM sleep period. No statistically significant difference was revealed in the mean O2 saturation percentage (p < 0.001). Watch PAT is an efficient device and is considered to be an adjunctive diagnostic method for PSG in diagnosis of OSAS.

  4. PAT1, a new member of the GRAS family, is involved in phytochrome A signal transduction

    PubMed Central

    Bolle, Cordelia; Koncz, Csaba; Chua, Nam-Hai

    2000-01-01

    Light signaling via the phytochrome A (phyA) photoreceptor controls basic plant developmental processes including de-etiolation and hypocotyl elongation. We have identified a new Arabidopsis mutant, pat (phytochrome A signal transduction)1-1, which shows strongly reduced responses in continuous far-red light. Physiological and molecular data indicate that this mutant is disrupted at an early step of phyA signal transduction. The PAT1 gene encodes a cytoplasmic protein of 490 amino acids with sequence homologies to the plant-specific GRAS regulatory protein family. In the pat1-1 mutant, a T-DNA insertion introduces a premature stop codon, which likely results in the production of a truncated PAT1 protein of 341 amino acids. The semidominant phenotype of this mutant can be recapitulated by overexpression of an appropriately truncated PAT1 gene in the wild type. The results indicate that the truncated PAT1 protein acts in a dominant-negative fashion to inhibit phyA signaling. PMID:10817761

  5. The UK-DALE dataset, domestic appliance-level electricity demand and whole-house demand from five UK homes.

    PubMed

    Kelly, Jack; Knottenbelt, William

    2015-01-01

    Many countries are rolling out smart electricity meters. These measure a home's total power demand. However, research into consumer behaviour suggests that consumers are best able to improve their energy efficiency when provided with itemised, appliance-by-appliance consumption information. Energy disaggregation is a computational technique for estimating appliance-by-appliance energy consumption from a whole-house meter signal. To conduct research on disaggregation algorithms, researchers require data describing not just the aggregate demand per building but also the 'ground truth' demand of individual appliances. In this context, we present UK-DALE: an open-access dataset from the UK recording Domestic Appliance-Level Electricity at a sample rate of 16 kHz for the whole-house and at 1/6 Hz for individual appliances. This is the first open access UK dataset at this temporal resolution. We recorded from five houses, one of which was recorded for 655 days, the longest duration we are aware of for any energy dataset at this sample rate. We also describe the low-cost, open-source, wireless system we built for collecting our dataset.

  6. The UK-DALE dataset, domestic appliance-level electricity demand and whole-house demand from five UK homes

    PubMed Central

    Kelly, Jack; Knottenbelt, William

    2015-01-01

    Many countries are rolling out smart electricity meters. These measure a home’s total power demand. However, research into consumer behaviour suggests that consumers are best able to improve their energy efficiency when provided with itemised, appliance-by-appliance consumption information. Energy disaggregation is a computational technique for estimating appliance-by-appliance energy consumption from a whole-house meter signal. To conduct research on disaggregation algorithms, researchers require data describing not just the aggregate demand per building but also the ‘ground truth’ demand of individual appliances. In this context, we present UK-DALE: an open-access dataset from the UK recording Domestic Appliance-Level Electricity at a sample rate of 16 kHz for the whole-house and at 1/6 Hz for individual appliances. This is the first open access UK dataset at this temporal resolution. We recorded from five houses, one of which was recorded for 655 days, the longest duration we are aware of for any energy dataset at this sample rate. We also describe the low-cost, open-source, wireless system we built for collecting our dataset. PMID:25984347

  7. The UK-DALE dataset, domestic appliance-level electricity demand and whole-house demand from five UK homes.

    PubMed

    Kelly, Jack; Knottenbelt, William

    2015-01-01

    Many countries are rolling out smart electricity meters. These measure a home's total power demand. However, research into consumer behaviour suggests that consumers are best able to improve their energy efficiency when provided with itemised, appliance-by-appliance consumption information. Energy disaggregation is a computational technique for estimating appliance-by-appliance energy consumption from a whole-house meter signal. To conduct research on disaggregation algorithms, researchers require data describing not just the aggregate demand per building but also the 'ground truth' demand of individual appliances. In this context, we present UK-DALE: an open-access dataset from the UK recording Domestic Appliance-Level Electricity at a sample rate of 16 kHz for the whole-house and at 1/6 Hz for individual appliances. This is the first open access UK dataset at this temporal resolution. We recorded from five houses, one of which was recorded for 655 days, the longest duration we are aware of for any energy dataset at this sample rate. We also describe the low-cost, open-source, wireless system we built for collecting our dataset. PMID:25984347

  8. Enfermedad diarreica aguda por Escherichia coli patógenas en Colombia

    PubMed Central

    Gómez-Duarte, Oscar G.

    2014-01-01

    Resumen Las cepas de E. coli patógenas intestinales son causas importantes de la enfermedad diarreica aguda (EDA) en niños menores de 5 años en América Latina, África y Asia y están asociadas a alta mortalidad en niños en las comunidades más pobres de África y el Sudeste Asiático. Estudios sobre el papel de las E. coli patógenas intestinales en la EDA infantil en Colombia y otros países de América Latina son limitados debido a la carencia de ensayos para detección de estos patógenos en los laboratorios clínicos de centros de salud. Estudios recientes han reportado la detección de E. coli patógenas intestinales en Colombia, siendo la E. coli enterotoxigénica la cepa más frecuentemente asociada a diarrea en niños menores de 5 años. Otros patógenos detectados en estos pacientes incluyen las E. coli enteroagregativa, enteropatógena, productora de toxina Shiga, y de adherencia difusa. Con base en estudios que reportan la presencia de E. coli productora de toxina Shiga y E. coli enteroagregativa en carnes y vegetales en supermercados, se cree que productos alimentarios contaminados contribuyen a la transmisión de estos patógenos y a la infección del huésped susceptible. Más estudios son necesarios para evaluar los mecanismos de transmisión, el impacto en la epidemiologia de la EDA, y las pautas de manejo y prevención de estos patógenos que afectan la población pediátrica en Colombia. PMID:25491457

  9. The gene pat-2, which induces natural parthenocarpy, alters the gibberellin content in unpollinated tomato ovaries.

    PubMed

    Fos, M; Nuez, F; García-Martínez, J L

    2000-02-01

    We investigated the role of gibberellins (GAs) in the effect of pat-2, a recessive mutation that induces facultative parthenocarpic fruit development in tomato (Lycopersicon esculentum Mill.) using near-isogenic lines with two different genetic backgrounds. Unpollinated wild-type Madrigal (MA/wt) and Cuarenteno (CU/wt) ovaries degenerated, but GA(3) application induced parthenocarpic fruit growth. On the contrary, parthenocarpic growth of MA/pat-2 and CU/pat-2 fruits, which occurs in the absence of pollination and hormone application, was not affected by GA(3). Pollinated MA/wt and parthenocarpic MA/pat-2 ovary development was negated by paclobutrazol, and this inhibitory effect was counteracted by GA(3). The main GAs of the early-13-hydroxylation pathway (GA(1), GA(3), GA(8), GA(19), GA(20), GA(29), GA(44), GA(53), and, tentatively, GA(81)) and two GAs of the non-13-hydroxylation pathway (GA(9) and GA(34)) were identified in MA/wt ovaries by gas chromatography-selected ion monitoring. GAs were quantified in unpollinated ovaries at flower bud, pre-anthesis, and anthesis. In unpollinated MA/pat-2 and CU/pat-2 ovaries, the GA(20) content was much higher (up to 160 times higher) and the GA(19) content was lower than in the corresponding non-parthenocarpic ovaries. The application of an inhibitor of 2-oxoglutarate-dependent dioxygenases suggested that GA(20) is not active per se. The pat-2 mutation may increase GA 20-oxidase activity in unpollinated ovaries, leading to a higher synthesis of GA(20), the precursor of an active GA. PMID:10677440

  10. The gene pat-2, which induces natural parthenocarpy, alters the gibberellin content in unpollinated tomato ovaries.

    PubMed

    Fos, M; Nuez, F; García-Martínez, J L

    2000-02-01

    We investigated the role of gibberellins (GAs) in the effect of pat-2, a recessive mutation that induces facultative parthenocarpic fruit development in tomato (Lycopersicon esculentum Mill.) using near-isogenic lines with two different genetic backgrounds. Unpollinated wild-type Madrigal (MA/wt) and Cuarenteno (CU/wt) ovaries degenerated, but GA(3) application induced parthenocarpic fruit growth. On the contrary, parthenocarpic growth of MA/pat-2 and CU/pat-2 fruits, which occurs in the absence of pollination and hormone application, was not affected by GA(3). Pollinated MA/wt and parthenocarpic MA/pat-2 ovary development was negated by paclobutrazol, and this inhibitory effect was counteracted by GA(3). The main GAs of the early-13-hydroxylation pathway (GA(1), GA(3), GA(8), GA(19), GA(20), GA(29), GA(44), GA(53), and, tentatively, GA(81)) and two GAs of the non-13-hydroxylation pathway (GA(9) and GA(34)) were identified in MA/wt ovaries by gas chromatography-selected ion monitoring. GAs were quantified in unpollinated ovaries at flower bud, pre-anthesis, and anthesis. In unpollinated MA/pat-2 and CU/pat-2 ovaries, the GA(20) content was much higher (up to 160 times higher) and the GA(19) content was lower than in the corresponding non-parthenocarpic ovaries. The application of an inhibitor of 2-oxoglutarate-dependent dioxygenases suggested that GA(20) is not active per se. The pat-2 mutation may increase GA 20-oxidase activity in unpollinated ovaries, leading to a higher synthesis of GA(20), the precursor of an active GA.

  11. Clinical Usefulness of Watch-PAT for Assessing the Surgical Results of Obstructive Sleep Apnea Syndrome

    PubMed Central

    Park, Chong Yoon; Hong, Joon Hyeong; Lee, Jae Heon; Lee, Kyu Eun; Cho, Hyun Sang; Lim, Su Jin; Kwak, Jin Wook; Kim, Kyung Soo; Kim, Hyun Jik

    2014-01-01

    Objective: This study aimed to assess the accuracy and clinical efficacy of a wrist-worn device that is based on peripheral arterial tonometry (watch-PAT) to evaluate the surgical results of obstructive sleep apnea (OSA) syndrome subjects. Study Design and Method: Thirty-five subjects who were diagnosed with OSA and underwent sleep surgeries such as septoplasty, tonsillectomy, or uvuloplasty to correct their airway collapse, participated in this study; the watch-PAT-derived respiratory disturbance index (RDI), apnea and hypopnea index (AHI), lowest oxygen saturation, and valid sleep time were measured after the sleep surgery. Results: The present study showed that RDI (32.8 ± 10.7 vs 14.8 ± 7.5), AHI (30.3 ± 8.6 vs 13.4 ± 8.2 events/h), lowest oxygen saturation (78.2% ± 8.4% vs 90.5% ± 7.1%), and valid sleep time (329.1 ± 47.2 min and a postoperative value of 389.1 ± 50.1 min) recovered to within a normal range after surgery in 28 subjects. In addition, good agreement was found between watch-PAT-derived factors and visual analogue scales for changes in subjective symptoms, such as snoring, apnea, and daytime somnolence. Seven of the 35 subjects showed no improvement for their subjective symptoms and complained of snoring and apnea after surgery. We found that the RDI and AHI of those 7 subjects were not reduced, and the changes between pre- and postoperative values which were measured with watch-PAT were minimal. Their postoperative lowest oxygen saturation and valid sleep time were not elevated per the watch-PAT. The results support a strong correlation between the findings from watch-PAT and improved symptoms after surgical correction of an airway collapse. Conclusions: Our study provides evidence that the factors measured by the watch-PAT might be reliable indicators of symptomatic changes in OSA subjects after sleep surgery and also shows that the watch-PAT is a highly sensitive portable device for estimating treatment results in OSA. Citation: Park CY

  12. PAT: a protein analysis toolkit for integrated biocomputing on the web

    PubMed Central

    Gracy, Jérôme; Chiche, Laurent

    2005-01-01

    PAT, for Protein Analysis Toolkit, is an integrated biocomputing server. The main goal of its design was to facilitate the combination of different processing tools for complex protein analyses and to simplify the automation of repetitive tasks. The PAT server provides a standardized web interface to a wide range of protein analysis tools. It is designed as a streamlined analysis environment that implements many features which strongly simplify studies dealing with protein sequences and structures and improve productivity. PAT is able to read and write data in many bioinformatics formats and to create any desired pipeline by seamlessly sending the output of a tool to the input of another tool. PAT can retrieve protein entries from identifier-based queries by using pre-computed database indexes. Users can easily formulate complex queries combining different analysis tools with few mouse clicks, or via a dedicated macro language, and a web session manager provides direct access to any temporary file generated during the user session. PAT is freely accessible on the Internet at . PMID:15980554

  13. Monitoring granulation rate processes using three PAT tools in a pilot-scale fluidized bed.

    PubMed

    Tok, Ai Tee; Goh, Xueping; Ng, Wai Kiong; Tan, Reginald B H

    2008-01-01

    The purpose of this research was to analyze and compare the responses of three Process Analytical Technology (PAT) techniques applied simultaneously to monitor a pilot-scale fluidized bed granulation process. Real-time measurements using focused beam reflectance measurement (Lasentec FBRM) and near-infra red spectroscopy (Bruker NIR) were taken by inserting in-line probes into the fluidized bed. Non-intrusive acoustic emission measurements (Physical Acoustic AE) were performed by attaching piezoelectric sensors on the external wall of the fluidized bed. Powder samples were collected at regular intervals during the granulation process and characterized offline using laser diffraction, scanning electron microscopy, stereo-optical microscopy and loss on drying method. PAT data comprising chord length distribution and chord count (from FBRM), absorption spectra (from NIR) and average signal levels and counts (from AE) were compared with the particle properties measured using offline samples. All three PAT techniques were able to detect the three granulation regimes or rate processes (wetting and nucleation, consolidation and growth, breakage) to varying degrees of sensitivity. Being dependent on optical signals, the sensitivities of the FBRM and NIR techniques were susceptible to fouling on probe windows. The AE technique was sensitive to background fluidizing air flows and external interferences. The sensitivity, strengths and weaknesses of the PAT techniques examined may facilitate the selection of suitable PAT tools for process development and scale-up studies.

  14. PAT proteins, an ancient family of lipid droplet proteins that regulate cellular lipid stores

    PubMed Central

    Bickel, Perry E.; Tansey, John T.; Welte, Michael A.

    2009-01-01

    Summary The PAT family of lipid droplet proteins includes 5 members in mammals: perilipin, adipose differentiation-related protein (ADRP), tail-interacting protein of 47 kiloDaltons (TIP47), S3-12, and OXPAT. Members of this family are also present in evolutionarily distant organisms, including insects, slime molds and fungi. All PAT proteins share sequence similarity and the ability to bind intracellular lipid droplets, either constitutively or in response to metabolic stimuli, such as increased lipid flux into or out of lipid droplets. Positioned at the lipid droplet surface, PAT proteins manage access of other proteins (lipases) to the lipid esters within the lipid droplet core and can interact with cellular machinery important for lipid droplet biogenesis. Genetic variations in the gene for the best characterized of the mammalian PAT proteins, perilipin, have been associated with metabolic phenotypes, including type 2 diabetes mellitus and obesity. In this review, we discuss how the PAT proteins regulate cellular lipid metabolism both in mammals and in model organisms. PMID:19375517

  15. The Protein Acetyltransferase PatZ from Escherichia coli Is Regulated by Autoacetylation-induced Oligomerization*

    PubMed Central

    de Diego Puente, Teresa; Gallego-Jara, Julia; Castaño-Cerezo, Sara; Bernal Sánchez, Vicente; Fernández Espín, Vanesa; García de la Torre, José; Manjón Rubio, Arturo; Cánovas Díaz, Manuel

    2015-01-01

    Lysine acetylation is an important post-translational modification in the metabolic regulation of both prokaryotes and eukaryotes. In Escherichia coli, PatZ (formerly YfiQ) is the only known acetyltransferase protein and is responsible for acetyl-CoA synthetase acetylation. In this study, we demonstrated PatZ-positive cooperativity in response to acetyl-CoA and the regulation of acetyl-CoA synthetase activity by the acetylation level. Furthermore, functional analysis of an E809A mutant showed that the conserved glutamate residue is not relevant for the PatZ catalytic mechanism. Biophysical studies demonstrated that PatZ is a stable tetramer in solution and is transformed to its octameric form by autoacetylation. Moreover, this modification is reversed by the sirtuin CobB. Finally, an in silico PatZ tetramerization model based on hydrophobic and electrostatic interactions is proposed and validated by three-dimensional hydrodynamic analysis. These data reveal, for the first time, the structural regulation of an acetyltransferase by autoacetylation in a prokaryotic organism. PMID:26251518

  16. The Protein Acetyltransferase PatZ from Escherichia coli Is Regulated by Autoacetylation-induced Oligomerization.

    PubMed

    de Diego Puente, Teresa; Gallego-Jara, Julia; Castaño-Cerezo, Sara; Bernal Sánchez, Vicente; Fernández Espín, Vanesa; García de la Torre, José; Manjón Rubio, Arturo; Cánovas Díaz, Manuel

    2015-09-18

    Lysine acetylation is an important post-translational modification in the metabolic regulation of both prokaryotes and eukaryotes. In Escherichia coli, PatZ (formerly YfiQ) is the only known acetyltransferase protein and is responsible for acetyl-CoA synthetase acetylation. In this study, we demonstrated PatZ-positive cooperativity in response to acetyl-CoA and the regulation of acetyl-CoA synthetase activity by the acetylation level. Furthermore, functional analysis of an E809A mutant showed that the conserved glutamate residue is not relevant for the PatZ catalytic mechanism. Biophysical studies demonstrated that PatZ is a stable tetramer in solution and is transformed to its octameric form by autoacetylation. Moreover, this modification is reversed by the sirtuin CobB. Finally, an in silico PatZ tetramerization model based on hydrophobic and electrostatic interactions is proposed and validated by three-dimensional hydrodynamic analysis. These data reveal, for the first time, the structural regulation of an acetyltransferase by autoacetylation in a prokaryotic organism.

  17. Simulation model and on-ground performances validation of the PAT system for SILEX program

    NASA Astrophysics Data System (ADS)

    Cosson, Francois; Doubrere, Patrick; Perez, Eric

    1991-06-01

    A description of the simulation model and the on-ground performances validation of the Acquisition and Tracking (PAT) subsystem and of the European SILEX program is presented. The SILEX scenario is composed of two terminals mounted on LEO orbit (SPOT 4) and geostationary orbit (ARTEMIS) spacecrafts. The PAT subsystem is in charge of the initialization of the link, of the tracking of the incoming beam and of the pointing of the emitted laser beam. A short description of the retained strategies (phases of the PAT mission and operating modes) and functional architecture (control laws and sampling frequencies) is presented. In order to validate the PAT performance assessment, a complete simulation software is developed. The model (equipment modeling, dynamical simulation of the terminal structure, and operating modes management) and the associated outputs are examined in detail. The chosen design and architecture of the PAT validation on a system testbed (STB) are described. The validation of a pointing system for optical intersatellite communication, thanks to the combination of simulations and STB measurements, is shown.

  18. PAT proteins, an ancient family of lipid droplet proteins that regulate cellular lipid stores.

    PubMed

    Bickel, Perry E; Tansey, John T; Welte, Michael A

    2009-06-01

    The PAT family of lipid droplet proteins includes 5 members in mammals: perilipin, adipose differentiation-related protein (ADRP), tail-interacting protein of 47 kDa (TIP47), S3-12, and OXPAT. Members of this family are also present in evolutionarily distant organisms, including insects, slime molds and fungi. All PAT proteins share sequence similarity and the ability to bind intracellular lipid droplets, either constitutively or in response to metabolic stimuli, such as increased lipid flux into or out of lipid droplets. Positioned at the lipid droplet surface, PAT proteins manage access of other proteins (lipases) to the lipid esters within the lipid droplet core and can interact with cellular machinery important for lipid droplet biogenesis. Genetic variations in the gene for the best-characterized of the mammalian PAT proteins, perilipin, have been associated with metabolic phenotypes, including type 2 diabetes mellitus and obesity. In this review, we discuss how the PAT proteins regulate cellular lipid metabolism both in mammals and in model organisms. PMID:19375517

  19. A Novel Gene Amplification Causes Upregulation of the PatAB ABC Transporter and Fluoroquinolone Resistance in Streptococcus pneumoniae

    PubMed Central

    Baylay, Alison J.; Ivens, Alasdair

    2015-01-01

    Overexpression of the ABC transporter genes patA and patB confers efflux-mediated fluoroquinolone resistance in Streptococcus pneumoniae and is also linked to pneumococcal stress responses. Although upregulation of patAB has been observed in many laboratory mutants and clinical isolates, the regulatory mechanisms controlling expression of these genes are unknown. In this study, we aimed to identify the cause of high-level constitutive overexpression of patAB in M184, a multidrug-resistant mutant of S. pneumoniae R6. Using a whole-genome transformation and sequencing approach, we identified a novel duplication of a 9.2-kb region of the M184 genome which included the patAB genes. This duplication did not affect growth and was semistable with a low segregation rate. The expression levels of patAB in M184 were much higher than those that could be fully explained by doubling of the gene dosage alone, and inactivation of the first copy of patA had no effect on multidrug resistance. Using a green fluorescent protein reporter system, increased patAB expression was ascribed to transcriptional read-through from a tRNA gene upstream of the second copy of patAB. This is the first report of a large genomic duplication causing antibiotic resistance in S. pneumoniae and also of a genomic duplication causing antibiotic resistance by a promoter switching mechanism. PMID:25779578

  20. Progress Report on Student Learning Outcomes Assessment. A Report to the Maryland Higher Education Commission from Howard Community College.

    ERIC Educational Resources Information Center

    Howard Community Coll., Columbia, MD. Office of Planning and Evaluation.

    Illustrates progress made at Howard Community College (HCC) in the last three years in efforts to improve student learning, instructional effectiveness, and curriculum. Information for this progress report was compiled with input from offices involved in monitoring outcomes assessment of student learning at the college. These include the office of…

  1. The Survey of Employers of 1986 Howard Community College Graduates: A Report of the Findings. Research Report Number 53.

    ERIC Educational Resources Information Center

    Livieratos, Barbara B.

    A study was conducted at Howard Community College (HCC) to determine employers' perceptions of the employment preparation and job training provided by HCC. Questionnaires were mailed to the employers of 38 1986 graduates, who had previously granted permission for their employers to be contacted. Study findings, based on a 68% response rate,…

  2. Capacity-Building for African American Mental Health Training and Research: Lessons from the Howard-Dartmouth Collaborative Summer School

    ERIC Educational Resources Information Center

    Hipolito, Maria M. S.; Malik, Mansoor; Carpenter-Song, Elizabeth; Whitley, Rob

    2012-01-01

    Background: Many psychiatric residents have traditionally received little-or-no training in cross cultural approaches to psychiatric training and research. Method: The Dartmouth-Howard Collaboration summer school training program had a 5-year grant to explore approaches to enhancing understanding of cultural factors in mental health treatment and…

  3. The Validation of the Hillside Assessment of Perceived Intelligences (HAPI): A Measure of Howard Gardner's Theory of Multiple Intelligences.

    ERIC Educational Resources Information Center

    Shearer, C. Branton; Jones, James A.

    Howard Gardner's multiple intelligence (MI) theory is an alternative to the unitary concept of general intelligence, but it lacks a practical, reliable, and valid method of assessment. The Hillside Assessment of Perceived Intelligences (HAPI) is an attempt to measure the seven constructs of MI theory with an objective, psychometrically sound…

  4. What to Teach about Asia: Howard Wilson and the Committee on Asiatic Studies in the 1940s

    ERIC Educational Resources Information Center

    Shaffer, Robert

    2001-01-01

    In 1942, Howard Wilson, a professor at the Harvard Graduate School of Education and the editor of the Harvard Educational Review, called for the "easternization of America," in reaction to what he called the "glib" talk for years about the "westernization of Asia." Funded by the Rockefeller Foundation, Wilson's Committee, Committee on Asiatic…

  5. The Power-Load-Margin Formula of Howard Y. McClusky as the Basis for a Model of Teaching.

    ERIC Educational Resources Information Center

    Main, Keith

    1979-01-01

    Examines the writings of Howard McClusky and his power-load-margin (PLM) formula as the nucleus for a teaching-learning model that fosters mutual respect, shared responsibility, and a spirit of mutual inquiry in small groups or individualized learning experiences. Four phases which form the syntax of the PLM model are presented. (LRA)

  6. Communicating with New and Existing Markets. A Task Force of Howard Community College's 1998-1999 Commission on the Future.

    ERIC Educational Resources Information Center

    Howard Community Coll., Columbia, MD.

    This is a report from a task force formed by Howard Community College (Maryland) to examine existing and future markets. The task force also explored ways to use marketing strategies to attract potential customers to the college. The task force recommends that the college use its strengths to attract customers, such as its commitment to open…

  7. Overview of the High Performance Antiproton Trap (HiPAT) Experiment

    NASA Technical Reports Server (NTRS)

    Martin, James; Chakrabarti, Suman; Pearson, Boise; Sims, W. Herbert; Lewis, Raymond; Fant, Wallace; Rodgers, Stephen (Technical Monitor)

    2002-01-01

    A general overview of the High Performance Antiproton Trap (HiPAT) Experiment is presented. The topics include: 1) Why Antimatter? 2) HiPAT Applicability; 3) Approach-Goals; 4) HiPAT General Layout; 5) Sizing For Containment; 6) Laboratory Operations; 7) Vacuum System Cleaning; 8) Ion Production Via Electron Gun; 9) Particle Capture Via Ion Sources; 10) Ion Beam Steering/Focusing; 11) Ideal Ion Stacking Sequence; 12) Setup For Dynamic Capture; 13) Dynamic Capture of H(+) Ions; 14) Dynamic Capture; 15) Radio Frequency Particle Detection; 16) Radio Frequency Antenna Modeling; and 17) R.F. Stabilization-Low Frequencies. A short presentation of propulsion applications of Antimatter is also given. This paper is in viewgraph form.

  8. RF Manipulation and Detection of Protons in the High Performance Antiproton Trap (HiPAT) Experiment

    NASA Technical Reports Server (NTRS)

    Martin, James; Lewis, Raymond A.; Pearson, J. Boise; Sims, W. Herb; Chakrabarti, Suman; Fant, Wallace E.; Stan McDonald

    2003-01-01

    HiPAT is a Penning-Malmberg trap designed to contain 10(exp 12) particles diagnostics of the plasma. Destructive particle detection is performed by extracting trapped ions from HiPAT and colliding them with a microchannel plate detector (providing number and energy information). The improved RF system has been used to detect various plasma modes for both electron and ion plasmas in the two traps at MSFC, including axd, cyclotron, and diocotron modes. New diagnostics are also being added to HiPAT to measure the axial density distribution of the trapped cloud to match measured RF plasma modes to plasma conditions. In addition, plasma modeling efforts have been started using the XOOPIC code.

  9. Palaeomagnetism of the Howards Pass Zn-Pb deposits, Yukon, Canada

    NASA Astrophysics Data System (ADS)

    Kawasaki, K.; Symons, D. T. A.

    2012-07-01

    The Howards Pass district is located in the Selwyn Basin, Yukon. The district consists of >15 laminated clastic-dominated (CD) sphalerite-galena (Zn-Pb) deposits in the 'zinc corridor' that trends northwest-southeast and extends for ˜35 km. The stratiform mineralized zone, the Active Member, is hosted in carbonaceous cherts and black shales of the Early Silurian Road River Group. Using mostly thermal and then alternating field step demagnetization isolated a stable characteristic remanent magnetization (ChRM) by the core-magnetization-angle method from 18 sites (339 specimens) in 18 variably oriented exploration drill hole cores from 6 Zn-Pb mineralized panels. Rock magnetic analyses show that the main remanence carriers are single- or pseudosingle-domain pyrrhotite and titanomagnetite. The deposits' mean ChRM direction yields a pole position of either ˜170 Ma on the North American apparent polar wander path or ˜162 Ma on a corrected path for the Intermontane Belt (IMB) terranes. A negative palaeomagnetic fold test indicates that the mineralization's ChRM is post-folding, setting a minimum age for regional metamorphic deformation of ˜170 ± 20 Ma and supporting an Early Jurassic arrival for the IMB's collision and accretion to North America. An autochthonous or para-autochthonous North American tectonic model is favoured for the ChRM of the metamorphosed Selwyn Basin strata rather than an allochthonous IMB model. Further, the palaeomagnetic age indicates that the coarse-grained Zn-Pb mineralization in fine fractures that cut the laminated fine-grained Zn-Pb mineralization at Howards Pass at least was formed by remobilization during Middle Jurassic metamorphism.

  10. Water Vapor Measurements by Howard University Raman Lidar during the WAVES 2006 Campaign

    NASA Technical Reports Server (NTRS)

    Adam, M.; Demoz, B. B.; Whiteman, D. N.; Venable, D. D.; Joseph E.; Gambacorta, A.; Wei, J.; Shephard, M. W.; Miloshevich, L. M.; Barnet, C. D.; Herman, R. L.; Fitzgibbon, J.; Connell, R.

    2009-01-01

    Retrieval of water vapor mixing ratio using the Howard University Raman Lidar is presented with emphasis on three aspects: i) performance of the lidar against collocated radiosondes and Raman lidar, ii) investigation of the atmospheric state variables when poor agreement between lidar and radiosondes values occurred and iii) a comparison with satellite-based measurements. The measurements were acquired during the Water Vapor Validation Experiment Sondes/Satellites 2006 field campaign. Ensemble averaging of water vapor mixing ratio data from ten night-time comparisons with Vaisala RS92 radiosondes shows on average an agreement within 10 % up to approx. 8 km. A similar analysis of lidar-to-lidar data of over 700 profiles revealed an agreement to within 20 % over the first 7 km (10 % below 4 km). A grid analysis, defined in the temperature - relative humidity space, was developed to characterize the lidar - radiosonde agreement and quantitatively localizes regions of strong and weak correlations as a function of altitude, temperature or relative humidity. Three main regions of weak correlation emerge: i) regions of low relative humidity and low temperature, ii) moderate relative humidity at low temperatures and iii) low relative humidity at moderate temperatures. Comparison of Atmospheric InfraRed Sounder and Tropospheric Emission Sounder satellites retrievals of moisture with that of Howard University Raman Lidar showed a general agreement in the trend but the formers miss a lot of the details in atmospheric structure due to their low resolution. A relative difference of about 20 % is usually found between lidar and satellites measurements.

  11. The effects of PAT on the Savannah River ecosystem, particularly fisheries

    SciTech Connect

    Patrick, R.

    1994-03-01

    The main purpose of this study was to determine whether or not the pre-startup activities at K-Reactor, i.e., Power Ascension Testing (PAT), have caused damage because of temperature rises in the Savannah River. Therefore, the biological studies were mainly aimed at providing information as to changes that might cause the damage of the fish population, and to other important organisms in the ecosystem. To determine if deleterious effects had occurred, one had to review the past studies to determine the condition and diversity of aquatic life before these PAT studies started. Therefore old reports were reviewed and a current study made in 1992.

  12. The PATS Peer Support Program: Prevention/Early Intervention for Adolescents Who Have a Parent with Mental Illness

    ERIC Educational Resources Information Center

    Hargreaves, John; Bond, Lyndal; O'Brien, Matt; Forer, Danielle; Davies, Liz

    2008-01-01

    PATS (Paying Attention to Self) is a peer support program for adolescent children of parents with a diagnosed mental illness. The program aims to promote positive mental health, reduce the likelihood of mental health difficulties, increase young people's coping skills and empower them to meet their own and their families' needs. PATS combines peer…

  13. RNA-related nuclear functions of human Pat1b, the P-body mRNA decay factor.

    PubMed

    Marnef, Aline; Weil, Dominique; Standart, Nancy

    2012-01-01

    The evolutionarily conserved Pat1 proteins are P-body components recently shown to play important roles in cytoplasmic gene expression control. Using human cell lines, we demonstrate that human Pat1b is a shuttling protein whose nuclear export is mediated via a consensus NES sequence and Crm1, as evidenced by leptomycin B (LMB) treatment. However, not all P-body components are nucleocytoplasmic proteins; rck/p54, Dcp1a, Edc3, Ge-1, and Xrn1 are insensitive to LMB and remain cytoplasmic in its presence. Nuclear Pat1b localizes to PML-associated foci and SC35-containing splicing speckles in a transcription-dependent manner, whereas in the absence of RNA synthesis, Pat1b redistributes to crescent-shaped nucleolar caps. Furthermore, inhibition of splicing by spliceostatin A leads to the reorganization of SC35 speckles, which is closely mirrored by Pat1b, indicating that it may also be involved in splicing processes. Of interest, Pat1b retention in these three nuclear compartments is mediated via distinct regions of the protein. Examination of the nuclear distribution of 4E-T(ransporter), an additional P-body nucleocytoplasmic protein, revealed that 4E-T colocalizes with Pat1b in PML-associated foci but not in nucleolar caps. Taken together, our findings strongly suggest that Pat1b participates in several RNA-related nuclear processes in addition to its multiple regulatory roles in the cytoplasm.

  14. Design and Fabrication of Cryostat Interface and Electronics for High Performance Antimatter Trap (HI-PAT)

    NASA Technical Reports Server (NTRS)

    Smith, Gerald A.

    1999-01-01

    Included in Appendix I to this report is a complete set of design and assembly schematics for the high vacuum inner trap assembly, cryostat interfaces and electronic components for the MSFC HI-PAT. Also included in the final report are summaries of vacuum tests, and electronic tests performed upon completion of the assembly.

  15. Comparing EndoPAT and BIOPAC measurement of vascular responses to mental stress.

    PubMed

    Martin, Elizabeth A; Nelson, Rebecca E; Felmlee-Devine, M Donna; Brown, Troy E; Lerman, Amir

    2011-06-01

    There are currently no comparison measurements of stress-induced changes in vascular function during acute mental stress tests to measurements made by BIOPAC MP150 systems technology, a standard polygraph device used to detect deception during polygraph examinations in military or law enforcement applications. Vascular responses to reactive hyperaemia and acute mental stress in 25 healthy subjects were measured by both peripheral arterial tonometry (EndoPAT) and a blood pressure cuff attached to a pressure transducer (BIOPAC) and compared. Reactive hyperaemia was performed at baseline and following three acute mental stress tests. There was no difference in vascular reactivity at baseline and following acute mental stress, as measured by EndoPAT or BIOPAC systems (p > 0·05). Mental stress ratios measured by EndoPAT were significantly different than those measured by BIOPAC (p < 0·01). These data suggest that EndoPAT measurements of vascular responses to acute mental stress may be more specific and sensitive than measurements using the BIOPAC system. PMID:21671245

  16. Reconstruction of undersampled radial PatLoc imaging using Total Generalized Variation

    PubMed Central

    Knoll, Florian; Schultz, Gerrit; Bredies, Kristian; Gallichan, Daniel; Zaitsev, Maxim; Hennig, Jürgen; Stollberger, Rudolf

    2016-01-01

    In the case of radial imaging with nonlinear spatial encoding fields, a prominent star-shaped artifact has been observed if a spin distribution is encoded with an undersampled trajectory. This work presents a new iterative reconstruction method based on the total generalized variation (TGV), which reduces this artifact. For this approach, a sampling operator (as well as its adjoint) is needed that maps data from PatLoc k-space to the final image space. It is shown that this can be realized as a Type-3 non-uniform FFT, which is implemented by a combination of a Type-1 and Type-2 non-uniform FFT. Using this operator, it is also possible to implement an iterative conjugate gradient (CG) SENSE based method for PatLoc reconstruction, which leads to a significant reduction of computation time in comparison to conventional PatLoc image reconstruction methods. Results from numerical simulations and in-vivo PatLoc measurements with as few as 16 radial projections are presented, which demonstrate significant improvements in image quality with the TGV based approach. PMID:22847824

  17. Quantifying beetle-mediated effects on gas fluxes from dung pats.

    PubMed

    Penttilä, Atte; Slade, Eleanor M; Simojoki, Asko; Riutta, Terhi; Minkkinen, Kari; Roslin, Tomas

    2013-01-01

    Agriculture is one of the largest contributors of the anthropogenic greenhouse gases (GHGs) responsible for global warming. Measurements of gas fluxes from dung pats suggest that dung is a source of GHGs, but whether these emissions are modified by arthropods has not been studied. A closed chamber system was used to measure the fluxes of carbon dioxide (CO2), methane (CH4) and nitrous oxide (N2O) from dung pats with and without dung beetles on a grass sward. The presence of dung beetles significantly affected the fluxes of GHGs from dung pats. Most importantly, fresh dung pats emitted higher amounts of CO2 and lower amounts of CH4 per day in the presence than absence of beetles. Emissions of N2O showed a distinct peak three weeks after the start of the experiment--a pattern detected only in the presence of beetles. When summed over the main grazing season (June-July), total emissions of CH4 proved significantly lower, and total emissions of N2O significantly higher in the presence than absence of beetles. While clearly conditional on the experimental conditions, the patterns observed here reveal a potential impact of dung beetles on gas fluxes realized at a small spatial scale, and thereby suggest that arthropods may have an overall effect on gas fluxes from agriculture. Dissecting the exact mechanisms behind these effects, mapping out the range of conditions under which they occur, and quantifying effect sizes under variable environmental conditions emerge as key priorities for further research.

  18. Teacher's PAT? Multiple-Role Principal-Agent Theory, Education Politics, and Bureaucrat Power

    ERIC Educational Resources Information Center

    Vanhuysse, Pieter; Sulitzeanu-Kenan, Raanan

    2009-01-01

    This article aims to contribute to current debates about political power and agency relationships in education and other public sectors. In a recent clarion call for a major redirection of political principal-agent theories (PAT), Terry Moe has argued that standard information asymmetries ought no longer to be regarded as the sole foundation of…

  19. Draft Genome Sequence of the Beer Spoilage Bacterium Megasphaera cerevisiae Strain PAT 1T

    PubMed Central

    Kutumbaka, Kirthi K.; Pasmowitz, Joshua; Mategko, James; Reyes, Dindo; Friedrich, Alex; Han, Sukkyun; Martens-Habbena, Willm; Neal-McKinney, Jason; Janagama, Harish K.; Nadala, Cesar

    2015-01-01

    The genus Megasphaera harbors important spoilage organisms that cause beer spoilage by producing off flavors, undesirable aroma, and turbidity. Megasphaera cerevisiae is mainly found in nonpasteurized low-alcohol beer. In this study, we report the draft genome of the type strain of the genus, M. cerevisiae strain PAT 1T. PMID:26358606

  20. Draft Genome Sequence of the Beer Spoilage Bacterium Megasphaera cerevisiae Strain PAT 1T.

    PubMed

    Kutumbaka, Kirthi K; Pasmowitz, Joshua; Mategko, James; Reyes, Dindo; Friedrich, Alex; Han, Sukkyun; Martens-Habbena, Willm; Neal-McKinney, Jason; Janagama, Harish K; Nadala, Cesar; Samadpour, Mansour

    2015-01-01

    The genus Megasphaera harbors important spoilage organisms that cause beer spoilage by producing off flavors, undesirable aroma, and turbidity. Megasphaera cerevisiae is mainly found in nonpasteurized low-alcohol beer. In this study, we report the draft genome of the type strain of the genus, M. cerevisiae strain PAT 1(T). PMID:26358606

  1. PhyloPat: an updated version of the phylogenetic pattern database contains gene neighborhood.

    PubMed

    Hulsen, Tim; Groenen, Peter M A; de Vlieg, Jacob; Alkema, Wynand

    2009-01-01

    Phylogenetic patterns show the presence or absence of certain genes in a set of full genomes derived from different species. They can also be used to determine sets of genes that occur only in certain evolutionary branches. Previously, we presented a database named PhyloPat which allows the complete Ensembl gene database to be queried using phylogenetic patterns. Here, we describe an updated version of PhyloPat which can be queried by an improved web server. We used a single linkage clustering algorithm to create 241,697 phylogenetic lineages, using all the orthologies provided by Ensembl v49. PhyloPat offers the possibility of querying with binary phylogenetic patterns or regular expressions, or through a phylogenetic tree of the 39 included species. Users can also input a list of Ensembl, EMBL, EntrezGene or HGNC IDs to check which phylogenetic lineage any gene belongs to. A link to the FatiGO web interface has been incorporated in the HTML output. For each gene, the surrounding genes on the chromosome, color coded according to their phylogenetic lineage can be viewed, as well as FASTA files of the peptide sequences of each lineage. Furthermore, lists of omnipresent, polypresent, oligopresent and anticorrelating genes have been included. PhyloPat is freely available at http://www.cmbi.ru.nl/phylopat.

  2. Project-based Modules from two STEM Learning Teams in Howard County, Maryland

    NASA Astrophysics Data System (ADS)

    Griffiths, L. N.; Bradley, L. A.

    2011-12-01

    In 2009, two Maryland school districts-Howard County Public School System and Prince George's County Public Schools-and the Goddard Space Flight Center of the National Aeronautics and Space Administration (NASA) partnered with the National Commission on Teaching and America's Future (NCTAF) to develop NASA 21st Century Learning Studios. In 2010, NCTAF expanded the program to include Learning Studios at two additional Maryland school districts (Anne Arundel County Public Schools and Baltimore County Public Schools), partnering with the United States Naval Academy and the University of Maryland. Overall, the focus of these Learning Studios is to combine the expertise of scientists with that of educators through Learning Teams to improve teaching and learning in science, technology, engineering and mathematics (STEM) fields, while delivering project-based modules to be implemented in other school districts. The focus of this paper is to summarize the experience and outcomes from two Learning Teams from the Howard County Public School System. STEM Learning Teams were established at Centennial High School and Hammond High School in Maryland. Each Team worked together for two years to create interdisciplinary units of study for their students with a focus on Earth Science. To maximize student interest, teachers worked with NASA scientists five times a year to develop four learning modules using practical examples and incorporating real scientific observations. A weathering and erosion module challenges students to collect appropriate field observations and determine erosion and deposition rates in a nearby lake. A plate tectonics module requires students to use measures of plate motion from the National Oceanic and Atmospheric Administration to estimate rates of convergence in southern Asia. A third module for lessons in climate change requires students to find open source climate data, determine changes in the atmosphere and estimate anthropogenic impacts. A follow

  3. Learning to collaborate: the teaching legacy of Howard Zonana in forensic psychiatry.

    PubMed

    Wizner, Stephen

    2010-01-01

    The teaching legacy of Howard Zonana in forensic psychiatry has been in educating psychiatric fellows and law students to collaborate in advocacy on behalf of individuals whose problems exist at the intersection of the law and mental health. The author describes more than three decades of collaborative teaching with Dr. Zonana in Yale Law School legal clinics serving mental hospital patients, prisoners, children, and immigrants. In those clinics, law students and forensic psychiatry fellows have worked collaboratively in representing clinic clients and advocating for their legal and medical rights. The article reports three examples of this interdisciplinary collaboration: a project conducted at a state hospital shortly after the Supreme Court's decision in O'Connor v. Donaldson; a class action on behalf of individuals with developmental disabilities inappropriately subjected to long-term confinement in a state hospital; and an asylum case on behalf of a severely traumatized African woman fleeing persecution in her home country. The author concludes that Dr. Zonana's legacy reflects not only his contributions to medical and legal education, but also his profound commitment to effective and humane medical practice and to justice.

  4. Principles and Practices Fostering Inclusive Excellence: Lessons from the Howard Hughes Medical Institute's Capstone Institutions.

    PubMed

    DiBartolo, Patricia Marten; Gregg-Jolly, Leslie; Gross, Deborah; Manduca, Cathryn A; Iverson, Ellen; Cooke, David B; Davis, Gregory K; Davidson, Cameron; Hertz, Paul E; Hibbard, Lisa; Ireland, Shubha K; Mader, Catherine; Pai, Aditi; Raps, Shirley; Siwicki, Kathleen; Swartz, Jim E

    2016-01-01

    Best-practices pedagogy in science, technology, engineering, and mathematics (STEM) aims for inclusive excellence that fosters student persistence. This paper describes principles of inclusivity across 11 primarily undergraduate institutions designated as Capstone Awardees in Howard Hughes Medical Institute's (HHMI) 2012 competition. The Capstones represent a range of institutional missions, student profiles, and geographical locations. Each successfully directed activities toward persistence of STEM students, especially those from traditionally underrepresented groups, through a set of common elements: mentoring programs to build community; research experiences to strengthen scientific skill/identity; attention to quantitative skills; and outreach/bridge programs to broaden the student pool. This paper grounds these program elements in learning theory, emphasizing their essential principles with examples of how they were implemented within institutional contexts. We also describe common assessment approaches that in many cases informed programming and created traction for stakeholder buy-in. The lessons learned from our shared experiences in pursuit of inclusive excellence, including the resources housed on our companion website, can inform others' efforts to increase access to and persistence in STEM in higher education. PMID:27562960

  5. B. C. Canyon field, Howard County, Texas: An ancient analogy to modern tropical tower karst terrains

    SciTech Connect

    Mozynski, D.C.; Reid, A.M. )

    1992-04-01

    Late in the early deposition of sediments in Canyon field, a series of glacio-eustatically controlled sea level lowstands resulted in a carbonate buildup seaward of the Horseshoe Atoll in Howard County, Texas. The resulting satellite reef tract consists of fringing boundstone; high-energy shelf grainstones; lower energy shelf packstones and wackestones; and thin, highstand, black shales and mudstones. The original extent and thickness of deposits were extensively modified during karstification coincident with successive sea level lowstands. The resulting paleotopographic landforms appear to be similar to tower karst features of Puerto Rico. During the beginning of each sea level highstand, the paleoterrain was modified by erosion. The basinal foreshelf conglomerates resulting from initial highstand erosion contain dipping strata that commonly can be detected by the dipmeter tool. The mechanism for the formation of these strata may be depositional or the result of diagenetic alteration of the rock fabric in the burial environment. Using dipmeter data, an uneconomic producer has been offset by one of the better producing wells in the field.

  6. Cannabis careers revisited: applying Howard S. Becker's theory to present-day cannabis use.

    PubMed

    Järvinen, Margaretha; Ravn, Signe

    2014-01-01

    A considerable part of today's sociological research on recreational drug use is (explicitly or implicitly) inspired by Howard Becker's classical model of deviant careers. The aim of the present paper is to directly apply Becker's theory to empirical data on present-day cannabis use and to suggest a revision of the theory. As part of this, we propose a stretch of the sociological approach represented by Becker and followers in order to include, not only recreational drug use, but also use for which young people have sought treatment. The paper is based on 30 qualitative interviews with young people in treatment for cannabis problems in Copenhagen, Denmark. We suggest a revision of Becker's career model in relation to four aspects: initiation of cannabis use, differentiation between socially integrated and individualised, disintegrated use, social control from non-users, and the users' moral stance on cannabis. A central point of the paper is that social interaction may both motivate cannabis use, as Becker proposed, and serve as a protective factor against extensive, problematic use.

  7. Principles and Practices Fostering Inclusive Excellence: Lessons from the Howard Hughes Medical Institute's Capstone Institutions.

    PubMed

    DiBartolo, Patricia Marten; Gregg-Jolly, Leslie; Gross, Deborah; Manduca, Cathryn A; Iverson, Ellen; Cooke, David B; Davis, Gregory K; Davidson, Cameron; Hertz, Paul E; Hibbard, Lisa; Ireland, Shubha K; Mader, Catherine; Pai, Aditi; Raps, Shirley; Siwicki, Kathleen; Swartz, Jim E

    2016-01-01

    Best-practices pedagogy in science, technology, engineering, and mathematics (STEM) aims for inclusive excellence that fosters student persistence. This paper describes principles of inclusivity across 11 primarily undergraduate institutions designated as Capstone Awardees in Howard Hughes Medical Institute's (HHMI) 2012 competition. The Capstones represent a range of institutional missions, student profiles, and geographical locations. Each successfully directed activities toward persistence of STEM students, especially those from traditionally underrepresented groups, through a set of common elements: mentoring programs to build community; research experiences to strengthen scientific skill/identity; attention to quantitative skills; and outreach/bridge programs to broaden the student pool. This paper grounds these program elements in learning theory, emphasizing their essential principles with examples of how they were implemented within institutional contexts. We also describe common assessment approaches that in many cases informed programming and created traction for stakeholder buy-in. The lessons learned from our shared experiences in pursuit of inclusive excellence, including the resources housed on our companion website, can inform others' efforts to increase access to and persistence in STEM in higher education.

  8. PAT-2 (Plutonium Air-Transportable Model 2) safety analysis report

    SciTech Connect

    Andersen, J.A.; Davis, E.J.; Duffey, T.A.; Dupree, S.A.; George, O.L. Jr.; Ortiz, Z.

    1981-07-01

    The PAT-2 package is designed for the safe transport of plutonium and/or uranium in small quantities. The PAT-2 package is resistant to severe accidents, including that of a high-speed jet aircraft crash, and is designed to withstand such environments as extreme impact, crushing, puncturing and slashing loads, severe hydrocarbon-fueled fires, and deep underwater immersion, with no escape of contents. The package meets the requirements of 10 CFR 71 for Fissile Class I packages with a cargo of 15 grams of Pu-239, or other isotopic forms described herein, not to exceed 2 watts of thermal activity. This SAR presents design and oprational information including evaluations and analyses, test results, operating procedures, maintenance, and quality assurance information.

  9. PAT-1 safety analysis report addendum author responses to request for additional information.

    SciTech Connect

    Weiner, Ruth F.; Schmale, David T.; Kalan, Robert J.; Akin, Lili A.; Miller, David Russell; Knorovsky, Gerald Albert; Yoshimura, Richard Hiroyuki; Lopez, Carlos; Harding, David Cameron; Jones, Perry L.; Morrow, Charles W.

    2010-09-01

    The Plutonium Air Transportable Package, Model PAT-1, is certified under Title 10, Code of Federal Regulations Part 71 by the U.S. Nuclear Regulatory Commission (NRC) per Certificate of Compliance (CoC) USA/0361B(U)F-96 (currently Revision 9). The National Nuclear Security Administration (NNSA) submitted SAND Report SAND2009-5822 to NRC that documented the incorporation of plutonium (Pu) metal as a new payload for the PAT-1 package. NRC responded with a Request for Additional Information (RAI), identifying information needed in connection with its review of the application. The purpose of this SAND report is to provide the authors responses to each RAI. SAND Report SAND2010-6106 containing the proposed changes to the Addendum is provided separately.

  10. Review of the High Performance Antiproton Trap (HiPAT) Experiment

    NASA Technical Reports Server (NTRS)

    Martin, James J.; Lewis, Raymond A.; Pearson, J. Boise; Sims, W. Herb; Chakrabarti, Suman; Fant, Wallace E.; McDonald, Stan

    2003-01-01

    Many space propulsion concepts exist that use matter-antimatter reactions. Current antiproton production rates are enough to conduct proof-of-principle evaluation of these concepts. One enabling technology for such experiments is portable storage of low energy antiprotons, to transport antiprotons to experimental facilities. To address this need, HiPAT is being developed, with a design goal of containing 10(exp 12) particles for up to 18 days. HiPAT is a Penning-Malmberg trap with a 4 Tesla superconductor, 20kV electrodes, radio frequency (RF) network, and 10(exp -13) Torr vacuum. 'Normal' matter is being used to evaluate the system. An electron beam ionizes background gas in situ, and particle beams are captured dynamically. The experiment examines ion storage lifetimes, RF plasma diagnostics, charge exchange with background gases, and dynamic ion beam capture.

  11. X-PAT: a multiplatform patient referral data management system for small healthcare institution requirements.

    PubMed

    Masseroli, Marco; Marchente, Mario

    2008-07-01

    We present X-PAT, a platform-independent software prototype that is able to manage patient referral multimedia data in an intranet network scenario according to the specific control procedures of a healthcare institution. It is a self-developed storage framework based on a file system, implemented in eXtensible Markup Language (XML) and PHP Hypertext Preprocessor Language, and addressed to the requirements of limited-dimension healthcare entities (small hospitals, private medical centers, outpatient clinics, and laboratories). In X-PAT, healthcare data descriptions, stored in a novel Referral Base Management System (RBMS) according to Health Level 7 Clinical Document Architecture Release 2 (CDA R2) standard, can be easily applied to the specific data and organizational procedures of a particular healthcare working environment thanks also to the use of standard clinical terminology. Managed data, centralized on a server, are structured in the RBMS schema using a flexible patient record and CDA healthcare referral document structures based on XML technology. A novel search engine allows defining and performing queries on stored data, whose rapid execution is ensured by expandable RBMS indexing structures. Healthcare personnel can interface the X-PAT system, according to applied state-of-the-art privacy and security measures, through friendly and intuitive Web pages that facilitate user acceptance. PMID:18632322

  12. Ion Dynamic Capture Experiments With The High Performance Antiproton Trap (HiPAT)

    NASA Technical Reports Server (NTRS)

    Martin, James; Lewis, Raymond; Chakrabarti, Suman; Sims, William H.; Pearson, J. Boise; Fant, Wallace E.

    2002-01-01

    To take the first step towards using the energy produced from the matter-antimatter annihilation for propulsion applications, the NASA Marshall Space Flight Center (MSFC) Propulsion Research Center (PRC) has initiated a research activity examining the storage of low energy antiprotons. The High Performance Antiproton Trap (HiPAT) is an electromagnetic system (Penning-Malmberg design) consisting of a 4 Tesla superconductor, a high voltage electrode confinement system, and an ultra high vacuum test section. It has been designed with an ultimate goal of maintaining 10(exp 12) charged particles with a half-life of 18 days. Currently, this system is being evaluated experimentally using normal matter ions that are cheap to produce, relatively easy to handle, and provide a good indication of overall trap behavior (with the exception of assessing annihilation losses). The ions are produced via a positive hydrogen ion source and transported to HiPAT in a beam line equipped with electrostatic optics. The optics serve to both focus and gate the incoming ions, providing microsecond-timed beam pulses that are dynamically captured by cycling the HiPAT forward containment field like a "trap door". Initial dynamic capture experiments have been successfully performed with beam energy and currents set to 1.9 kV and 23 micro-amps, respectively. At these settings up to 2x10(exp 9) ions have been trapped during a single dynamic cycle.

  13. A numerical model characterizing the experimental performance of the Howard University Raman Lidar system

    NASA Astrophysics Data System (ADS)

    Connell, Rasheen M.

    At the Howard University Atmospheric Observatory in Beltsville, MD, a Raman Lidar System was developed to provide both daytime and nighttime measurements of water vapor, aerosols, and cirrus clouds with 60 s temporal and 7.5 m spatial resolution in the lower and upper troposphere. This system analyzes signals at three wavelengths associated with Rayleigh/Mie scattering for aerosols and cirrus clouds at 354.7 nm, Raman scattering for nitrogen at 386.7 nm, and water vapor at 407.5 nm. The transmitter is a triple harmonic Nd: YAG solid state laser. The receiver is a 40 cm Cassegrain telescope. The detector system consists of a multi-channel wavelength separator unit and data acquisition system. This thesis develops a numerical model to provide a realistic representation of the system behavior. The variants of the lidar equation in the model use system parameters to solve and determine the return signals for the lidar system. This dissertation describes four case studies being investigated: clear sky, polluted, wet, and cirrus cloud atmospheric conditions. The first simulations are based on a standard atmosphere, which assumes an unpolluted (aerosol-free) dry-air atmosphere. The second and third sets of simulations are based on polluted and cirrus cloud atmospheric conditions, where aerosols and cirrus clouds are added to Case Study I. The last set of simulations is based on a wet atmosphere, where the troposphere is comprised of the same mixture of gases in Case Study II, with the addition of atmospheric water vapor. Lidar signals are simulated over the altitude range covered by our measurements (up to 14 km). Results of our simulations show that the measured and modeled signals agree within 10% over an extended period of time when the system (i.e., such as alignment, filter tuning, etc.) has not changed.

  14. Ion Storage Tests with the High Performance Antimatter Trap (HiPAT)

    NASA Technical Reports Server (NTRS)

    Martin, James J.; Lewis, Raymond A.; Chakrabarti, Suman; Pearson, Boise; Schafer, Charles (Technical Monitor)

    2002-01-01

    The NASA/Marshall Space Flight Centers (NASA/MSFC) Propulsion Research Center (PRC) is evaluating an antiproton storage system, referred to as the High Performance Antiproton Trap (HiPAT). This interest stems from the sheer energy represented by matter/antimatter annihilation process with has an energy density approximately 10 order of magnitude above that of chemical propellants. In other terms, one gram of antiprotons contains the equivalent energy of approximately 23 space shuttle external tanks or ET's (each ET contains roughly 740,000 kgs of fuel and oxidizer). This incredible source of stored energy, if harnessed, would be an enabling technology for deep space mission where both spacecraft weight and propulsion performance are key to satisfying aggressive mission requirements. The HiPAT hardware consists of a 4 Tesla superconductor system, an ultra high vacuum test section (vacuum approaching 10(exp -12) torr), and a high voltage confinement electrode system (up to 20 kvolts operation). The current laboratory layout is illustrated. The HiPAT designed objectives included storage of up to 1 trillion antiprotons with corresponding lifetimes approaching 18 days. To date, testing has centered on the storage of positive hydrogen ions produced in situ by a stream of high-energy electrons that passes through the trapping region. However, due to space charge issues and electron beam compression as it passes through the HiPAT central field, current ion production is limited to less then 50,000 ions. Ion lifetime was determined by counting particle populations at the end of various storage time intervals. Particle detection was accomplished by destructively expelling the ions against a micro-channel plate located just outside the traps magnetic field. The effect of radio frequency (RF) stabilization on the lifetime of trapped particles was also examined. This technique, referred to as a rotating wall, made use of a segmented electrode located near the center of the trap

  15. Expression of PAT and NPT II proteins during the developmental stages of a genetically modified pepper developed in Korea.

    PubMed

    Kim, Hyo Jin; Lee, Si Myung; Kim, Jae Kwang; Ryu, Tae Hun; Suh, Seok Cheol; Cho, Hyun Suk

    2010-10-27

    Estimation of the protein levels introduced in a biotechnology-derived product is conducted as part of an overall safety assessment. An enzyme-linked immunosorbent assay (ELISA) was used to analyze phosphinothricin acetyltransferase (PAT) and neomycin phosphotransferase II (NPT II) protein expression in a genetically modified (GM) pepper plant developed in Korea. PAT and NPT II expression levels, based on both dry weight and fresh weight, were variable among different plant generations and plant sections from isolated genetically modified organism (GMO) fields at four developmental stages. PAT expression was highest in leaves at anthesis (11.44 μg/gdw and 2.17 μg/gfw) and lowest in roots (0.12 μg/gdw and 0.01 μg/gfw). NPT II expression was also highest in leaves at anthesis (17.31 μg/gdw and 3.41 μg/gfw) and lowest in red pepper (0.65 μg/gdw and 0.12 μg/gfw). In pollen, PAT expression was 0.59-0.62 μg/gdw, while NPT II was not detected. Both PAT and NPT II showed a general pattern of decreased expression with progression of the growing season. As expected, PAT and NPT II protein expression was not detectable in control pepper plants.

  16. The Lsm1-7-Pat1 complex promotes viral RNA translation and replication by differential mechanisms

    PubMed Central

    Jungfleisch, Jennifer; Chowdhury, Ashis; Alves-Rodrigues, Isabel; Tharun, Sundaresan; Díez, Juana

    2015-01-01

    The Lsm1-7-Pat1 complex binds to the 3′ end of cellular mRNAs and promotes 3′ end protection and 5′–3′ decay. Interestingly, this complex also specifically binds to cis-acting regulatory sequences of viral positive-strand RNA genomes promoting their translation and subsequent recruitment from translation to replication. Yet, how the Lsm1-7-Pat1 complex regulates these two processes remains elusive. Here, we show that Lsm1-7-Pat1 complex acts differentially in these processes. By using a collection of well-characterized lsm1 mutant alleles and a system that allows the replication of Brome mosaic virus (BMV) in yeast we show that the Lsm1-7-Pat1 complex integrity is essential for both, translation and recruitment. However, the intrinsic RNA-binding ability of the complex is only required for translation. Consistent with an RNA-binding-independent function of the Lsm1-7-Pat1 complex on BMV RNA recruitment, we show that the BMV 1a protein, the sole viral protein required for recruitment, interacts with this complex in an RNA-independent manner. Together, these results support a model wherein Lsm1-7-Pat1 complex binds consecutively to BMV RNA regulatory sequences and the 1a protein to promote viral RNA translation and later recruitment out of the host translation machinery to the viral replication complexes. PMID:26092942

  17. The Lsm1-7-Pat1 complex promotes viral RNA translation and replication by differential mechanisms.

    PubMed

    Jungfleisch, Jennifer; Chowdhury, Ashis; Alves-Rodrigues, Isabel; Tharun, Sundaresan; Díez, Juana

    2015-08-01

    The Lsm1-7-Pat1 complex binds to the 3' end of cellular mRNAs and promotes 3' end protection and 5'-3' decay. Interestingly, this complex also specifically binds to cis-acting regulatory sequences of viral positive-strand RNA genomes promoting their translation and subsequent recruitment from translation to replication. Yet, how the Lsm1-7-Pat1 complex regulates these two processes remains elusive. Here, we show that Lsm1-7-Pat1 complex acts differentially in these processes. By using a collection of well-characterized lsm1 mutant alleles and a system that allows the replication of Brome mosaic virus (BMV) in yeast we show that the Lsm1-7-Pat1 complex integrity is essential for both, translation and recruitment. However, the intrinsic RNA-binding ability of the complex is only required for translation. Consistent with an RNA-binding-independent function of the Lsm1-7-Pat1 complex on BMV RNA recruitment, we show that the BMV 1a protein, the sole viral protein required for recruitment, interacts with this complex in an RNA-independent manner. Together, these results support a model wherein Lsm1-7-Pat1 complex binds consecutively to BMV RNA regulatory sequences and the 1a protein to promote viral RNA translation and later recruitment out of the host translation machinery to the viral replication complexes.

  18. Comparison of Tc-99m labeled liver and liver paté as markers for solid-phase gastric emptying.

    PubMed

    Christian, P E; Moore, J G; Datz, F L

    1984-03-01

    A radionuclide marker for studies of solid-phase gastric emptying should have a high labeling efficiency and remain relatively stable during gastric emptying. The availability of materials and the ease of preparation are also considerations in selecting radionuclide markers. We have compared the stability of intracellularly labeled chicken liver, surface-labeled chicken liver, and labeled puréed meat (liver paté) incubated with hydrochloric acid solution or gastric juice. Intracellularly labeled chicken liver and labeled liver paté were also compared in gastric emptying studies in humans. Our in vitro results demonstrated labeling efficiencies greater than 92% for both intracellularly labeled liver and labeled liver paté. The paté labeled with Tc-99m sulfur colloid was more stable than Tc-99m surface-labeled liver in vitro and its preparation was easier than with the intracellular labeling technique. Gastric emptying studies on normal subjects demonstrated equal performance of the intracellularly labeled liver and the labeled liver paté. Labeled liver paté is thus an alternative to intracellularly labeled chicken liver in measuring solid-phase gastric emptying.

  19. Transcriptomic analysis of tomato carpel development reveals alterations in ethylene and gibberellin synthesis during pat3/pat4 parthenocarpic fruit set

    PubMed Central

    Pascual, Laura; Blanca, Jose M; Cañizares, Joaquin; Nuez, Fernado

    2009-01-01

    Background Tomato fruit set is a key process that has a great economic impact on crop production. We employed the Affymetrix GeneChip Tomato Genome Array to compare the transcriptome of a non-parthenocarpic line, UC82, with that of the parthenocarpic line RP75/59 (pat3/pat4 mutant). We analyzed the transcriptome under normal conditions as well as with forced parthenocarpic development in RP75/59, emasculating the flowers 2 days before anthesis. This analysis helps to understand the fruit set in tomato. Results Differentially expressed genes were extracted with maSigPro, which is designed for the analysis of single and multiseries time course microarray experiments. 2842 genes showed changes throughout normal carpel development and fruit set. Most of them showed a change of expression at or after anthesis. The main differences between lines were concentrated at the anthesis stage. We found 758 genes differentially expressed in parthenocarpic fruit set. Among these genes we detected cell cycle-related genes that were still activated at anthesis in the parthenocarpic line, which shows the lack of arrest in the parthenocarpic line at anthesis. Key genes for the synthesis of gibberellins and ethylene, which were up-regulated in the parthenocarpic line were also detected. Conclusion Comparisons between array experiments determined that anthesis was the most different stage and the key point at which most of the genes were modulated. In the parthenocarpic line, anthesis seemed to be a short transitional stage to fruit set. In this line, the high GAs contends leads to the development of a parthenocarpic fruit, and ethylene may mimic pollination signals, inducing auxin synthesis in the ovary and the development of a jelly fruit. PMID:19480705

  20. Ten-year follow-up of a prospective trial for the targeted therapy of gastric cancer with the human monoclonal antibody PAT-SC1.

    PubMed

    Hensel, Frank; Timmermann, Wolfgang; von Rahden, Burkhard H A; Rosenwald, Andreas; Brändlein, Stephanie; Illert, Bertram

    2014-03-01

    The fully human monoclonal antibody PAT-SC1 is specific for an isoform of CD55 (decay-accelerating factor) designated CD55PAT-SC1. This antigen is expressed in the majority (80%) of gastric cancers (GCs), and the antibody induces tumour cell-specific apoptosis in vitro as well as in vivo. PAT-SC1, therefore, has been deemed promising as a therapeutic agent. Here, we describe the results of an academic clinical study performed in a neoadjuvant setting with resectable GC patients. Patients undergoing treatment for GC between 1997 and 2001 were tested for CD55PAT-SC1 expression. Fifty-one resectable patients that tested positively received a single administration of 20 mg PAT-SC1 48 h prior to surgery. They underwent standard surgery with either subtotal or total gastrectomy with bursectomy, omentectomy and a modified D2-lymphadenectomy, aimed at R0 resection. Primary endpoints of the present study were to evaluate side-effects of the PAT-SC1 antibody treatment and to evaluate histopathological effects such as tumour regression and induction of apoptosis. Long-term survival was a secondary endpoint. Administration of PAT-SC1 appeared safe with only reversible side-effects according to WHO grade I and II. Despite the low‑dose of the antibody, 81.6% of the patients showed signs of increased apoptosis within the primary tumour and 60% showed signs of tumour cell regression. Comparison of the 10-year survival rates of the R0-resected CD55PAT-SC1-positive patients treated with the PAT-SC1 antibody with a historical collective of R0-resected CD55PAT-SC1-positive patients not treated with PAT-SC1 indicated a survival benefit in the treated patients. Furthermore, comparison of the patient survival of CD55PAT‑SC1-positive vs. CD55PAT-SC1-negative groups suggested that CD55PAT-SC1 antigen expression is an independent predictor of poor survival in a Cox regression analysis. Antibody PAT-SC1 may be a useful additive therapeutic agent in the treatment of patients with CD55PAT

  1. The Gene pat-2, Which Induces Natural Parthenocarpy, Alters the Gibberellin Content in Unpollinated Tomato Ovaries1

    PubMed Central

    Fos, Mariano; Nuez, Fernando; García-Martínez, José L.

    2000-01-01

    We investigated the role of gibberellins (GAs) in the effect of pat-2, a recessive mutation that induces facultative parthenocarpic fruit development in tomato (Lycopersicon esculentum Mill.) using near-isogenic lines with two different genetic backgrounds. Unpollinated wild-type Madrigal (MA/wt) and Cuarenteno (CU/wt) ovaries degenerated, but GA3 application induced parthenocarpic fruit growth. On the contrary, parthenocarpic growth of MA/pat-2 and CU/pat-2 fruits, which occurs in the absence of pollination and hormone application, was not affected by GA3. Pollinated MA/wt and parthenocarpic MA/pat-2 ovary development was negated by paclobutrazol, and this inhibitory effect was counteracted by GA3. The main GAs of the early-13-hydroxylation pathway (GA1, GA3, GA8, GA19, GA20, GA29, GA44, GA53, and, tentatively, GA81) and two GAs of the non-13-hydroxylation pathway (GA9 and GA34) were identified in MA/wt ovaries by gas chromatography-selected ion monitoring. GAs were quantified in unpollinated ovaries at flower bud, pre-anthesis, and anthesis. In unpollinated MA/pat-2 and CU/pat-2 ovaries, the GA20 content was much higher (up to 160 times higher) and the GA19 content was lower than in the corresponding non-parthenocarpic ovaries. The application of an inhibitor of 2-oxoglutarate-dependent dioxygenases suggested that GA20 is not active per se. The pat-2 mutation may increase GA 20-oxidase activity in unpollinated ovaries, leading to a higher synthesis of GA20, the precursor of an active GA. PMID:10677440

  2. Dispersion and Transport of Cryptosporidium Oocysts from Fecal Pats under Simulated Rainfall Events

    PubMed Central

    Davies, Cheryl M.; Ferguson, Christobel M.; Kaucner, Christine; Krogh, Martin; Altavilla, Nanda; Deere, Daniel A.; Ashbolt, Nicholas J.

    2004-01-01

    The dispersion and initial transport of Cryptosporidium oocysts from fecal pats were investigated during artificial rainfall events on intact soil blocks (1,500 by 900 by 300 mm). Rainfall events of 55 mm h−1 for 30 min and 25 mm h−1 for 180 min were applied to soil plots with artificial fecal pats seeded with approximately 107 oocysts. The soil plots were divided in two, with one side devoid of vegetation and the other left with natural vegetation cover. Each combination of event intensity and duration, vegetation status, and degree of slope (5° and 10°) was evaluated twice. Generally, a fivefold increase (P < 0.05) in runoff volume was generated on bare soil compared to vegetated soil, and significantly more infiltration, although highly variable, occurred through the vegetated soil blocks (P < 0.05). Runoff volume, event conditions (intensity and duration), vegetation status, degree of slope, and their interactions significantly affected the load of oocysts in the runoff. Surface runoff transported from 100.2 oocysts from vegetated loam soil (25-mm h−1, 180-min event on 10° slope) to up to 104.5 oocysts from unvegetated soil (55-mm h−1, 30-min event on 10° slope) over a 1-m distance. Surface soil samples downhill of the fecal pat contained significantly higher concentrations of oocysts on devegetated blocks than on vegetated blocks. Based on these results, there is a need to account for surface soil vegetation coverage as well as slope and rainfall runoff in future assessments of Cryptosporidium transport and when managing pathogen loads from stock grazing near streams within drinking water watersheds. PMID:14766600

  3. Stacking Multiple Ion Captures in The High Performance Antiproton Trap (HiPAT)

    NASA Technical Reports Server (NTRS)

    Martin, James J.; Lewis, Raymond A.; Sims, William H.; Chakrabarti, Suman; Pearson, Boise; Fant, Wallace E.

    2004-01-01

    The High performance Antiproton Trap (HiPAT) research project was initiated by the Marshall Space Flight Center's propulsion Research Center to examining the fundamental behavior of low energy antiprotons. Stored antiproton would ultimately be used for experimental demonstration of basic propulsive concepts. Matter-antimatter annihilation produces approximately 10(exp 8) MJ/g nearly 10 orders of magnitude more energy per unit mass than chemical based combustion, hence NASA's interest. To achieve containment, HiPAT utilizes a type of electromagnetic bottle know as a Penning trap positioned within an ultrahigh vacuum test section. Recently, the HiPAT hardware configuration has been enhanced to facilitate the capture of multiple normal matter ion burst. This endeavor is often referred to as "stacking" and used to increasing the number of captured particles. A prior normal matter experimental effort, successfully demonstrated the effectiveness of single burst capture. The stacking process is accomplished by manipulating the electric field generated by the confinement electrodes i.e. adjusting the well potential depth. These potential well values are initially configured to maximize the quantity of captured ions per burst; shallow wells with a depth of 100 volt or less (referenced to the incoming ion beam energy) are typically selected. Once captured, a cooling interval is required to reduce the energy of trapped particles below the lower extent of the "trap door" (or leading electrode) ion emitting potential. This is necessary such that a new burst of hot ions can be introduced while preventing those already inside from escaping. The cooling time is driven by a combination of mechanisms such as synchrotron radiation, background gas scattering, and resistive damping in a time scale on the order of minutes. A potential for reducing this hold period is to actively manipulate the electric field shape, using the power supply control system, to produce a deeper potential

  4. The Killer Will Remain Free: On Pat Parker and the Poetics of Madness.

    PubMed

    Ali, Kazim

    2015-01-01

    Poet and scholar Kazim Ali reads Pat Parker's Movement in Black intimately, one poet to another, uncovering the shadow-fact of the lives of most people of color: not only the anger that is somehow sublimated into every part of our lives but also the issue that carrying this feeling around has on our mental health itself-that "anger" and "madness" might have sources in one another. Ali concludes that Parker offers a brutal and clear-eyed and ultimately hopeful assessment of the conditions that were faced at the time, and even now, by communities of color. PMID:26075690

  5. EARLY IMPACT MELTING AND SPACE EXPOSURE HISTORY OF THE PAT91501 LCHONDRITE

    NASA Technical Reports Server (NTRS)

    Bogard, Donald D.; Garrison, D. H.; Herzog, G. F.; Xue, S.; Klein, J.; Middleton, R.

    2004-01-01

    Collisions probably occurred frequently in the early history of the asteroid belt. Their effects, which should be recorded in meteorites, must have included heating and melting along with shock alteration of mineral textures. Some non-chondritic meteorite types e.g., eucrites and IIE and IAB irons - do indeed give evidence of extensive impact heating more than 3.4 Gyr ago. The ordinary chondrites, in contrast, show little evidence of early impact heating. The Ar-Ar and Rb-Sr ages of ordinary chondrites that experienced intense shock are for the most part relatively young, many less than 1.5 Gyr. The numerous L-chondrites with Ar- Ar ages clustering near 0.5 Gy are a well-known example. One of them, the 105-kg Chico Lchondrite, shows the effects of unusually intense heating. It is approximately 60% impact melt and likely formed as a dyke beneath a large crater when the L-chondrite parent body underwent a very large impact approximately 0.5 Gyr ago. In rare instances, older shock dates are indicated for ordinary chondrites. Dixon et al show early impact resetting of Ar-Ar ages of a few LL-chondrites including MIL 99301 at 4.23 0.03 Gyr, but in none of these stones did shock lead to extensive melting. As of 2003, searches for chondritic melts attributable to early shock had turned up only the Shaw L-chondrite, which has an Ar-Ar age of approximately 4.42 Gyr. PAT91501 is an 8.55-kg L-chondrite containing vesicles and metal-troilite nodules. It is a unique, near-total impact melt, unshocked, depleted in siderophile and chalcophile elements, and contains only approximately 10% relic chondritic material. The authors conclude that PAT91501 crystallized rapidly and from a much more homogeneous melt than did Shaw. They suggest that PAT resembles Chico and likely formed as an impact melt vein within an impact crater. To define the history of PAT, we have determined its Ar-39-Ar-40 age and measured several radioactive and stable nuclides produced during its space exposure to

  6. Petrology and Geochemistry of LEW 88663 and PAT 91501: High Petrologic L Chondrites

    NASA Astrophysics Data System (ADS)

    Mittlefehldt, D. W.; Lindstrom, M. M.; Field, S. W.

    1993-07-01

    Primitive achondrites (e.g., Acapulco, Lodran) are believed to be highly metamorphosed chondritic materials, perhaps up to the point of anatexis in some types. Low petrologic grade equivalents of these achondrites are unknown, so the petrologic transition from chondritic to achondritic material cannot be documented. However, there are rare L chondrites of petrologic grade 7 that may have experienced igneous processes, and study of these may yield information relevant to the formation of primitive achondrites, and perhaps basaltic achondrites, from chondritic precursors. We have begun the study of the L7 chondrites LEW 88663 and PAT 91501 as part of our broader study of primitive achondrites. Here, we present our preliminary petrologic and geochemical data on these meteorites. Petrology and Mineral Compositions: LEW 88663 is a granular achondrite composed of equant, subhedral to anhedral olivine grains poikilitically enclosed in networks of orthopyroxene and plagioclase. Small grains of clinopyroxene are spatially associated with orthopyroxene. Troilite occurs as large anhedral and small rounded grains. The smaller troilite grains are associated with the orthopyroxene-plagioclase networks. PAT 91501 is a vesicular stone containing centimeter-sized troilite +/- metal nodules. Its texture consists of anhedral to euhedral olivine grains, anhedral orthopyroxene grains (some with euhedral clinopyroxene overgrowths), anhedral to euhedral clinopyroxene, and interstitial plagioclase and SiO2-Al2O3-K2O- rich glass. In some areas, olivine is poikilitically enclosed in orthopyroxene. Fine-grained troilite, metal, and euhedral chromite occur interstitial to the silicates. Average mineral compositions for LEW 88663 are olivine Fo(sub)75.8, orthopyroxene Wo(sub)3.4En(sub)76.2Fs(sub)20.4, clinopyroxene Wo(sub)42.6En(sub)47.8Fs(sub)9.6, plagioclase Ab(sub)75.0An(sub)21.6Or(sub)3.4. Mineral compositions for PAT 91501 are olivine Fo(sub)73.8, orthopyroxene Wo(sub)4.5En(sub)74.8Fs

  7. Tools to enhance public participation and confidence in the development of the Howard East aquifer water plan, Northern Territory

    NASA Astrophysics Data System (ADS)

    Jackson, Sue; Tan, Poh-Ling; Nolan, Sharna

    2012-12-01

    SummaryFor the first time in the Northern Territory's water management history it is facing the challenge of managing an over-allocated groundwater resource. Rapid rural residential and agricultural development in the Howard River catchment of Darwin's hinterland has increased competition for groundwater from the bore-field that supplements the capital's metropolitan water supply. This has generated tensions between different water users and precipitated a water allocation plan for the Howard East aquifer. Initial context analysis indicated a widespread lack of public understanding of groundwater systems and processes, leading to misconceptions about the origin of local groundwater resources, groundwater-surface water interactions, extraction rates and impacts. In addition there is a legacy of mistrust by some peri-urban community members of government-driven planning processes to manage groundwater resources. The main objective of this study was therefore to trial two planning tools suited to this context over a 15 month period: (i) an extended stakeholder analysis and (ii) the participatory development of a 3D visualisation model, via a process described as the participatory Groundwater Visualisation Tool (GVT). The tools assisted the water planning agency to better understand stakeholder needs and interests, contributed to popular scientific understandings of hydro-geological conditions and processes, as well as captured local knowledge and values in preparation for an open and effective planning process.

  8. Howard I. Maibach: extraordinary leadership in integrating key concepts underpinning our understanding of percutaneous absorption and occupational dermatology.

    PubMed

    Surber, C; Elsner, P; Singh, J

    2013-01-01

    The purpose of the present article is to briefly highlight some contributions of Prof. Howard I. Maibach to the field of dermatology. After a few introducing remarks regarding Howard's personal career, the article specifically reviews contributions to the understanding of percutaneous absorption and to occupational dermatology. He and his companions/coworkers established and introduced experimental prerequisites to better study and understand percutaneous absorption - both in vitro and in vivo. Not less influential was his contribution to occupational dermatology acting as a founding member of the International Contact Dermatitis Research Group and coinaugurating the North American Contact Dermatitis Group. These groups have been very active ever since. As an academic teacher, he inspired young colleagues to perform original research work and to establish their own working groups. He has done this most successfully with many fellows who worked with him over the years, and who are now leading departments or companies dedicated to dermatological research all over the world. Probably this is his most important and lasting achievement.

  9. The parthenocarpic fruit (pat) mutant of tomato (Lycopersicon esculentum Mill.) sets seedless fruits and has aberrant anther and ovule development.

    PubMed

    Mazzucato, A; Taddei, A R; Soressi, G P

    1998-01-01

    Among the different sources of genetic parthenocarpy described in tomato, the mutation referred to as parthenocarpic fruit (pat) is of particular interest because of its strong expressivity and because it confers earlier ripening, higher fruit set and enhanced fruit quality. As a pleiotropic effect, pat flowers have aberrantly developing androecia and reduced male and female fertility. In this work we extend the early description of the pat phenotype by investigating the expression of parthenocarpy in three different environments and by using light and scanning electron microscopy to analyse the development of male and female floral organs. The degree of parthenocarpy was high in the three experimental environments and was characterised by a precocious initiation of ovary growth to pre-anthesis floral stages. Aberrations in anther development were evident at flower bud stages and resulted in shorter, irregular and teratoid organs. Ectopic production of carpel-like structures bearing external ovules was evident in the most severely altered androecia. Analysis of ovule development revealed that a fraction of pat ovules becomes aberrant from very early stages, having defective integument growth. Meiosis was irregular in aberrant ovules and megaspore or gamete production was severely hampered. The described pat syndrome suggests that parthenocarpy in this mutant could be a secondary effect of a gene controlling, at early stages, organ identity and development. PMID:9389668

  10. Bridging the gap between PAT concepts and implementation: An integrated software platform for fermentation.

    PubMed

    Chopda, Viki R; Gomes, James; Rathore, Anurag S

    2016-01-01

    Bioreactor control significantly impacts both the amount and quality of the product being manufactured. The complexity of the control strategy that is implemented increases with reactor size, which may vary from thousands to tens of thousands of litres in commercial manufacturing. The Process Analytical Technology (PAT) initiative has highlighted the need for having robust monitoring tools and effective control schemes that are capable of taking real time information about the critical quality attributes (CQA) and the critical process parameters (CPP) and executing immediate response as soon as a deviation occurs. However, the limited flexibility that present commercial software packages offer creates a hurdle. Visual programming environments have gradually emerged as potential alternatives to the available text based languages. This paper showcases development of an integrated programme using a visual programming environment for a Sartorius BIOSTAT® B Plus 5L bioreactor through which various peripheral devices are interfaced. The proposed programme facilitates real-time access to data and allows for execution of control actions to follow the desired trajectory. Major benefits of such integrated software system include: (i) improved real time monitoring and control; (ii) reduced variability; (iii) improved performance; (iv) reduced operator-training time; (v) enhanced knowledge management; and (vi) easier PAT implementation. PMID:26647285

  11. Bridging the gap between PAT concepts and implementation: An integrated software platform for fermentation.

    PubMed

    Chopda, Viki R; Gomes, James; Rathore, Anurag S

    2016-01-01

    Bioreactor control significantly impacts both the amount and quality of the product being manufactured. The complexity of the control strategy that is implemented increases with reactor size, which may vary from thousands to tens of thousands of litres in commercial manufacturing. The Process Analytical Technology (PAT) initiative has highlighted the need for having robust monitoring tools and effective control schemes that are capable of taking real time information about the critical quality attributes (CQA) and the critical process parameters (CPP) and executing immediate response as soon as a deviation occurs. However, the limited flexibility that present commercial software packages offer creates a hurdle. Visual programming environments have gradually emerged as potential alternatives to the available text based languages. This paper showcases development of an integrated programme using a visual programming environment for a Sartorius BIOSTAT® B Plus 5L bioreactor through which various peripheral devices are interfaced. The proposed programme facilitates real-time access to data and allows for execution of control actions to follow the desired trajectory. Major benefits of such integrated software system include: (i) improved real time monitoring and control; (ii) reduced variability; (iii) improved performance; (iv) reduced operator-training time; (v) enhanced knowledge management; and (vi) easier PAT implementation.

  12. Development of Process Analytical Technology (PAT) methods for controlled release pellet coating.

    PubMed

    Avalle, P; Pollitt, M J; Bradley, K; Cooper, B; Pearce, G; Djemai, A; Fitzpatrick, S

    2014-07-01

    This work focused on the control of the manufacturing process for a controlled release (CR) pellet product, within a Quality by Design (QbD) framework. The manufacturing process was Wurster coating: firstly layering active pharmaceutical ingredient (API) onto sugar pellet cores and secondly a controlled release (CR) coating. For each of these two steps, development of a Process Analytical Technology (PAT) method is discussed and also a novel application of automated microscopy as the reference method. Ultimately, PAT methods should link to product performance and the two key Critical Quality Attributes (CQAs) for this CR product are assay and release rate, linked to the API and CR coating steps respectively. In this work, the link between near infra-red (NIR) spectra and those attributes was explored by chemometrics over the course of the coating process in a pilot scale industrial environment. Correlations were built between the NIR spectra and coating weight (for API amount), CR coating thickness and dissolution performance. These correlations allow the coating process to be monitored at-line and so better control of the product performance in line with QbD requirements.

  13. The structure of the cyanobactin domain of unknown function from PatG in the patellamide gene cluster

    PubMed Central

    Mann, Greg; Koehnke, Jesko; Bent, Andrew F.; Graham, Rachael; Houssen, Wael; Jaspars, Marcel; Schwarz-Linek, Uli; Naismith, James H.

    2014-01-01

    Patellamides are members of the cyanobactin family of ribosomally synthesized and post-translationally modified cyclic peptide natural products, many of which, including some patellamides, are biologically active. A detailed mechanistic understanding of the biosynthetic pathway would enable the construction of a biotechnological ‘toolkit’ to make novel analogues of patellamides that are not found in nature. All but two of the protein domains involved in patellamide biosynthesis have been characterized. The two domains of unknown function (DUFs) are homologous to each other and are found at the C-termini of the multi-domain proteins PatA and PatG. The domain sequence is found in all cyanobactin-biosynthetic pathways characterized to date, implying a functional role in cyanobactin biosynthesis. Here, the crystal structure of the PatG DUF domain is reported and its binding interactions with plausible substrates are investigated. PMID:25484206

  14. Building on and Honoring Forty Years of PBL Scholarship from Howard Barrows: A Scientometric, Large-Scale Data, and Visualization-Based Analysis

    ERIC Educational Resources Information Center

    Xian, Hanjun; Madhavan, Krishna

    2013-01-01

    Over the past forty years, Howard Barrows' contributions to PBL research have influenced and guided educational research and practice in a diversity of domains. It is necessary to make visible to all PBL scholars what has been accomplished, what is perceived as significant, and what is the scope of applicability for Barrows' groundbreaking…

  15. Why Race and Culture Matter in Schools, and Why We Need to "Get This Right": A Conversation with Dr. Tyrone Howard

    ERIC Educational Resources Information Center

    Clark, Pat; Zygmunt, Eva; Howard, Tyrone

    2016-01-01

    Tyrone Howard is Professor of Education at UCLA; Associate Dean of Equity, Diversity, and Inclusion; and former Director of Center X, which is where UCLA's teacher education program is housed. Center X provides a unique setting where researchers and practitioners collaborate to design and conduct programs that prepare and support K-12 teachers and…

  16. The free radical scavenging and antioxidant activities of pod and seed extract of Clitoria fairchildiana (Howard)- an underutilized legume.

    PubMed

    Annegowda, H V; Bhat, Rajeev; Tze, Liong Min; Karim, A A; Mansor, S M

    2013-06-01

    We evaluated the phenolic content and antioxidant capacities of pod and seed extracts (in methanol, ethanol, and water) of an underutilized legume, Clitoria fairchildiana (Howard). The antioxidant capacity of the extracts was determined using the ferric reducing antioxidant potential assay, and the free radical-scavenging capacity was evaluated using 2,2-diphenyl-1-picrylhydrazyl radical-scavenging and ABTS assays. In addition, the total flavonoids, flavonols, and tannin contents were also determined. Overall, the methanol extracts of the pod contained high concentration of phenolics and showed high antioxidant capacities compared to seed extracts. In addition, a positive correlation was found between total phenol and tannin versus antioxidant capacity. Results of the present study indicate pods and seeds of C. fairchildiana to possess rich amount of natural antioxidants, and can be further explored for their possible use as a natural additive in food or in pharmaceutical industries. PMID:24425949

  17. Numerical upper bounds on convective heat transport in a layer of fluid of finite Prandtl number: Confirmation of Howard's analytical asymptotic single-wave-number bound

    NASA Astrophysics Data System (ADS)

    Vitanov, Nikolay K.

    2005-10-01

    By means of the Howard-Busse method of the optimum theory of turbulence we investigate numerically the upper bounds on the Nusselt number in a heated-from-below horizontal layer of fluid of finite Prandtl number for the case of rigid boundaries. The bounds are obtained by the solutions of the Euler-Lagrange equations of a variational problem possessing up to three wave numbers. The obtained results are compared to the numerical results for the case of fluid layer with stress-free boundaries [N. K. Vitanov and F. H. Busse, "Upper bounds on heat transport in a horizontal fluid layer with stress-free boundaries," ZAMP 48, 310 (1997)] as well as to the numerical and analytical asymptotic results obtained by Howard ["Heat transport by turbulent convection," J. Fluid Mech. 17, 405 (1963)], Busse ["On Howard's upper bound for heat transport by turbulent convection," J. Fluid Mech. 37, 457 (1969)], and Strauss ["On the upper bounding approach to thermal convection at moderate Rayleigh numbers, II. Rigid boundaries," Dyn. Atm. Oceans 1, 77 (1976)]. We show that for low and intermediate Rayleigh numbers the numerical bounds are positioned below the analytical asymptotic bounds obtained by Howard and Busse. For large Rayleigh numbers the numerical bounds tend to approach the analytical asymptotic bounds. We confirm numerically the bound obtained by Howard for the case of one-wave-number solution of the Euler-Lagrange equations. As the region of validity of the results of the analytical asymptotic theory for solutions of the Euler-Lagrange equations with two and three wave numbers lies in the area of very high Rayleigh numbers the values of the second and third wave numbers are different from their analytical asymptotic values for the values of the Rayleigh number reached by the numerical computation.

  18. "Anything That Gets Me in My Heart": Pat Parker's Poetry of Justice.

    PubMed

    Green, David B

    2015-01-01

    This article argues that love and justice are interlocking themes that undergird and motivate the poetry and activism of the Black lesbian feminist Pat Parker. Parker was a prolific working-class poet, a committed Black lesbian feminist, and an international trailblazer whose poems, like her famous "Womanslaughter" discussed in this article, document the many injustices that Black women endured in an anti-Black, rabidly homophobic, and patriarchal U.S. during the last decades of the twentieth century. In a political moment where righteous cries of #BlackLivesMatter are heard across the United States I use this article to remind us all of the historical importance that Black lesbians played and continue to play in the struggles of anti-racist justice in America. PMID:26075686

  19. The mechanism of patellamide macrocyclization revealed by the characterization of the PatG macrocyclase domain

    PubMed Central

    Koehnke, Jesko; Bent, Andrew; Houssen, Wael E; Zollman, David; Morawitz, Falk; Shirran, Sally; Vendome, Jeremie; Nneoyiegbe, Ada F; Trembleau, Laurent; Botting, Catherine H; Smith, Margaret C M; Jaspars, Marcel; Naismith, James H

    2012-01-01

    Peptide macrocycles are found in many biologically active natural products. Their versatility, resistance to proteolysis and ability to traverse membranes has made them desirable molecules. Although technologies exist to synthesize such compounds, the full extent of diversity found among natural macrocycles has yet to be achieved synthetically. Cyanobactins are ribosomal peptide macrocycles encompassing an extraordinarily diverse range of ring sizes, amino acids and chemical modifications. We report the structure, biochemical characterization and initial engineering of the PatG macrocyclase domain of Prochloron sp. from the patellamide pathway that catalyzes the macrocyclization of linear peptides. The enzyme contains insertions in the subtilisin fold to allow it to recognize a three-residue signature, bind substrate in a preorganized and unusual conformation, shield an acyl-enzyme intermediate from water and catalyze peptide bond formation. The ability to macrocyclize a broad range of nonactivated substrates has wide biotechnology applications. PMID:22796963

  20. PAT: From Western solid dosage forms to Chinese materia medica preparations using NIR-CI.

    PubMed

    Zhou, Luwei; Xu, Manfei; Wu, Zhisheng; Shi, Xinyuan; Qiao, Yanjiang

    2016-01-01

    Near-infrared chemical imaging (NIR-CI) is an emerging technology that combines traditional near-infrared spectroscopy with chemical imaging. Therefore, NIR-CI can extract spectral information from pharmaceutical products and simultaneously visualize the spatial distribution of chemical components. The rapid and non-destructive features of NIR-CI make it an attractive process analytical technology (PAT) for identifying and monitoring critical control parameters during the pharmaceutical manufacturing process. This review mainly focuses on the pharmaceutical applications of NIR-CI in each unit operation during the manufacturing processes, from the Western solid dosage forms to the Chinese materia medica preparations. Finally, future applications of chemical imaging in the pharmaceutical industry are discussed.

  1. PAT1.1; Pinellas Action Tracking System; Tracks Audit Findings and Corrective Actions

    SciTech Connect

    Mellican, P.L.

    1993-04-09

    PAT was derived from a system that began at the Rocky Flats Plant and was further enhanced at the Mound Plant. Pinellas Plant obtained this system in 1990 to track Tiger Team Findings; it has been expanded to include new modules which encompass a wide range of related functions. Functionality includes tracking of findings and associated corrective actions from various sources such as line operations, self-assessments, oversight assessments, and external organizations. Other functionality includes Management Walk-About tracking, NEPA prioritization, Occurrence/incident Report corrective action tracking, and Management Action Item Tracking. The system utilizes state of the art relational database technology with pop-up windows for table lookups and entry of descriptive text. Standards such as assessment identification numbers, area designations, and finding category codes have been developed to provide enhanced query capabilities and the ability to group findings for trending purposes on a plant-wide basis.

  2. RF Manipulation of Ions in the High Performance Antiproton Trap (HiPAT)

    NASA Technical Reports Server (NTRS)

    Pearson, J. Boise; Martin, James J.; Sims, William H.; Chakrabarti, Suman; Lewis, Raymond A.; Rodgers, Stephen L. (Technical Monitor)

    2002-01-01

    The annihilation of antimatter provides the highest mass specific energy of any other known reaction. Proper harnessing of this energy holds great promise for future space propulsion systems. Many different propulsion concepts have been proposed that take advantage of antimatter, either using matter-antimatter as the primary fuel, or as a 'spark plug' for fusion and fission systems. In order to begin to address these concepts experimentally, a method of storing and transporting antimatter must be developed. The High Performance Antiproton Trap (HiPAT) is a first-generation storage and transportation device designed to store and transport 10(exp 12) antiprotons with a storage half-life of 18 days. It uses a Penning-Malmberg ion trap with a 4T magnetic field and 20 kV potential. This will enable researchers much more flexibility in the design of antimatter experiments related to propulsion. Ions cannot be stored indefinitely in a real trap, as ion cloud instabilities develop from imperfections in manufacturing and misalignments in assembly. Previous work has been done at both the National Institute of Standards and University of California in San Diego in using RF (radio frequency) signals to both diagnose and confine the ion cloud. Two electrodes in the trap have been segmented to allow both reception and transmission of RF waves in the ion cloud. Experiments are underway to determine the number of ions and density in the cloud by "listening" to protons contained in the HiPAT. Currently we believe the density of ions stored in the trap is roughly 10(exp 15) m(exp -3). Development of non-destructive techniques is vital to the project goals, enabling continuous monitoring of the quantities stored in the system. Experimental work is also being done in identifying RF transmission frequencies that can manipulate the density of the cloud, by exchanging energy and momentum between the RF wave and the ions. Preliminary experiments have demonstrated this interaction.

  3. mini-PAT (Peer Assessment Tool): A Valid Component of a National Assessment Programme in the UK?

    ERIC Educational Resources Information Center

    Archer, Julian; Norcini, John; Southgate, Lesley; Heard, Shelley; Davies, Helena

    2008-01-01

    Purpose: To design, implement and evaluate a multisource feedback instrument to assess Foundation trainees across the UK. Methods: mini-PAT (Peer Assessment Tool) was modified from SPRAT (Sheffield Peer Review Assessment Tool), an established multisource feedback (360 [degree]) instrument to assess more senior doctors, as part of a blueprinting…

  4. Use of a highly sensitive immunomarking system to characterize face fly (Diptera: Muscidae) dispersal from cow pats.

    PubMed

    Peck, George W; Ferguson, Holly J; Jones, Vincent P; O'Neal, Sally D; Walsh, Douglas B

    2014-02-01

    We tested an immunomarking system that used egg white as marker and enzyme-linked immunosorbent assay as a detection assay to characterize face fly (Musca autumnalis DeGeer) dispersal from cow pats in a pastured beef cattle operation. In microcage assays, adult flies acquired marker after contact with cow pats that were treated with marker and field aged up to 11 d. In arena assays on sprayed full-size cow pats, 77% of eclosed face flies acquired the marker. In a field-marking study, four applications of egg white marker were applied on freshly deposited cow pats over a summer at two peripheral paddocks to a main grazing pasture of ≍50 head of beef cattle. Of the 663 face flies captured, 108 were positive for the egg white marker (16.3%). Of the marked flies, ≍ twofold more male than female flies were captured. Sex-specific dispersal distances were roughly equal up to 450 m, with 11% of female flies dispersing >450 m. Dispersal capability of face flies is discussed in relation to efficacy of rotational grazing and other IPM strategies.

  5. The Catalytic Mechanism of the Marine-Derived Macrocyclase PatGmac.

    PubMed

    Brás, Natércia F; Ferreira, Pedro; Calixto, Ana R; Jaspars, Marcel; Houssen, Wael; Naismith, James H; Fernandes, Pedro A; Ramos, Maria J

    2016-09-01

    Cyclic peptides are a class of compounds with high therapeutic potential, possessing bioactivities including antitumor and antiviral (including anti-HIV). Despite their desirability, efficient design and production of these compounds has not been achieved to date. The catalytic mechanism of patellamide macrocyclization by the PatG macrocyclase domain has been computationally investigated by using quantum mechanics/molecular mechanics methodology, specifically ONIOM(M06/6-311++G(2d,2p):ff94//B3LYP/6-31G(d):ff94). The mechanism proposed herein begins with a proton transfer from Ser783 to His 618 and from the latter to Asp548. Nucleophilic attack of Ser783 on the substrate leads to the formation of an acyl-enzyme covalent complex. The leaving group Ala-Tyr-Asp-Gly (AYDG) of the substrate is protonated by the substrate's N terminus, leading to the breakage of the P1-P1' bond. Finally, the substrate's N terminus attacks the P1 residue, decomposing the acyl-enzyme complex forming the macrocycle. The formation and decomposition of the acyl-enzyme complex have the highest activation free energies (21.1 kcal mol(-1) and 19.8 kcal mol(-1) respectively), typical of serine proteases. Understanding the mechanism behind the macrocyclization of patellamides will be important to the application of the enzymes in the pharmaceutical and biotechnological industries. PMID:27389424

  6. Process analytical technology (PAT): quantification approaches in terahertz spectroscopy for pharmaceutical application.

    PubMed

    Wu, Huiquan; Heilweil, Edwin J; Hussain, Ajaz S; Khan, Mansoor A

    2008-02-01

    Terahertz (THz) spectroscopy and chemometric analysis of resultant absorption spectra in the 30-500 cm(-1) range has been applied to perform quantitative determination of both active ingredient and excipient concentrations of tablets. Tests were performed on a series of tablets composed of various concentrations and processes of theophylline formulated with lactose, magnesium stearate, starch or Avicel, and as a function of tablet hardness. Transmission spectra of polyethylene pellets derived from each of the samples were analyzed using three approaches. Spectral superposition method was used as an indirect measure to examine whether and when the interaction among various pharmaceutical components and the tableting history could be considered insignificant for quantification purpose. Spectral characteristic peak method was able to correlate peak maxima with correction for tablets having the same hardness. Multivariate analysis (PCR and PLS 1) was capable of correlating THz spectra with tablet concentrations. The predicted concentrations of independent samples using multivariate models agreed well with nominal concentrations. The best correlations were obtained using multivariate analysis. With these studies, the advantage of using multivariate approach was demonstrated for process analytical technology (PAT) application. Further, the feasibility of integrating THz spectroscopy and chemometrics for the purpose of quantifying pharmaceutical tablet concentrations was demonstrated. PMID:17722101

  7. Ion Storage with the High Performance Antiproton Trap (HiPAT)

    NASA Technical Reports Server (NTRS)

    Martin, James; Lewis, Raymond; Chakrabarti, Suman; Pearson, Boise

    2002-01-01

    The matter antimatter reaction represents the densest form of energy storage/release known to modern physics: as such it offers one of the most compact sources of power for future deep space exploration. To take the first steps along this path, NASA-Marshall Space Flight Center is developing a storage system referred to as the High Performance Antiproton Trap (HiPAT) with a goal of maintaining 10(exp 12) particles for up to 18 days. Experiments have been performed with this hardware using normal matter (positive hydrogen ions) to assess the device's ability to hold charged particles. These ions are currently created using an electron gun method to ionize background gas; however, this technique is limited by the quantity that can be captured. To circumvent this issue, an ion source is currently being commissioned which will greatly increase the number of ions captured and more closely simulate actual operations expected at an antiproton production facility. Ions have been produced, stored for various time intervals, and then extracted against detectors to measure species, quantity and energy. Radio frequency stabilization has been tested as a method to prolong ion lifetime: results show an increase in the baseline 1/e lifetime of trapped particles from hours to days. Impurities in the residual background gas (typically carbon-containing species CH4, CO, CO2, etc.) present a continuing problem by reducing the trapped hydrogen population through the mechanism of ion charge exchange.

  8. Investigating 3-body Decays of Cluster States with the PAT-TPC

    NASA Astrophysics Data System (ADS)

    Carpenter, Lisa; Ayyad Limonge, Y.; Bazin, D.; Beceiro-Novo, S.; Bradt, J.; Cortesi, M.; Mittig, W.; Ahn, T.; Kolata, J. J.; Meisel, Z.; Bechetti, F. D.; Fritsch, A.; Howard, A.

    2016-03-01

    Recent model calculations with most advanced methods for cluster states have shown the need of experimental data to probe the structure of light exotic nuclei, including those with α-clustering, such as 14C. The Prototype Active Target Time Projection Chamber (PAT-TPC) allows us to investigate these types of structures, giving access to the full excitation function with a single beam energy. This type of experiment measures resonances in 14C that can be compared to the models. With an improved Micromegas pad plane with a circular backgammon design we are able to investigate 3-body decays in addition to 2-body scattering. The measurements were carried out by resonant alpha-scattering on 10Be beam delivered by the TwinSol facility at the University of Notre Dame. We also observed the 3-body decay of the Hoyle State in 12C from a 12N or 12B beam with the same device. Preliminary results will be presented. This work is supported by the National Science Foundation.

  9. Network and user interface for PAT DOME virtual motion environment system

    NASA Technical Reports Server (NTRS)

    Worthington, J. W.; Duncan, K. M.; Crosier, W. G.

    1993-01-01

    The Device for Orientation and Motion Environments Preflight Adaptation Trainer (DOME PAT) provides astronauts a virtual microgravity sensory environment designed to help alleviate tye symptoms of space motion sickness (SMS). The system consists of four microcomputers networked to provide real time control, and an image generator (IG) driving a wide angle video display inside a dome structure. The spherical display demands distortion correction. The system is currently being modified with a new graphical user interface (GUI) and a new Silicon Graphics IG. This paper will concentrate on the new GUI and the networking scheme. The new GUI eliminates proprietary graphics hardware and software, and instead makes use of standard and low cost PC video (CGA) and off the shelf software (Microsoft's Quick C). Mouse selection for user input is supported. The new Silicon Graphics IG requires an Ethernet interface. The microcomputer known as the Real Time Controller (RTC), which has overall control of the system and is written in Ada, was modified to use the free public domain NCSA Telnet software for Ethernet communications with the Silicon Graphics IG. The RTC also maintains the original ARCNET communications through Novell Netware IPX with the rest of the system. The Telnet TCP/IP protocol was first used for real-time communication, but because of buffering problems the Telnet datagram (UDP) protocol needed to be implemented. Since the Telnet modules are written in C, the Adap pragma 'Interface' was used to interface with the network calls.

  10. Implementation of transmission NIR as a PAT tool for monitoring drug transformation during HME processing.

    PubMed

    Islam, Muhammad T; Scoutaris, Nikolaos; Maniruzzaman, Mohammed; Moradiya, Hiren G; Halsey, Sheelagh A; Bradley, Michael S A; Chowdhry, Babur Z; Snowden, Martin J; Douroumis, Dennis

    2015-10-01

    The aim of the work reported herein was to implement process analytical technology (PAT) tools during hot melt extrusion (HME) in order to obtain a better understanding of the relationship between HME processing parameters and the extruded formulations. For the first time two in-line NIR probes (transmission and reflectance) have been coupled with HME to monitor the extrusion of the water insoluble drug indomethacin (IND) in the presence of Soluplus (SOL) or Kollidon VA64 hydrophilic polymers. In-line extrusion monitoring of sheets, produced via a specially designed die, was conducted at various drug/polymer ratios and processing parameters. Characterisation of the extruded transparent sheets was also undertaken by using DSC, XRPD and Raman mapping. Analysis of the experimental findings revealed the production of molecular solutions where IND is homogeneously blended (ascertained by Raman mapping) in the polymer matrices, as it acts as a plasticizer for both hydrophilic polymers. PCA analysis of the recorded NIR signals showed that the screw speed used in HME affects the recorded spectra but not the homogeneity of the embedded drug in the polymer sheets. The IND/VA64 and IND/SOL extruded sheets displayed rapid dissolution rates with 80% and 30% of the IND being released, respectively within the first 20min.

  11. [Interaction between Acarophenax lacunatus (Cross & Krantz) (Prostigmata: Acarophenacidae) and Anisopteromalus calandrae (Howard) (Hymenoptera: Pteromalidae) on Rhyzopertha dominica (Fabricius) (Coleoptera: Bostrichidae)].

    PubMed

    Gonçalves, José R; Faroni, Lêda R D'A; Guedes, Raul N C; de Oliveira, Carlos R F; Garcia, Flávia M

    2006-01-01

    The interaction between Acarophenax lacunatus (Cross & Krantz) and Anisopteromalus calandrae (Howard) may be a promising tool for the integrated pest management of stored grain insect pests. The objective of this study was to evaluate the compatibility of these two natural enemies on Rhyzopertha dominica (Fabricius). The experimental units were petri dishes (140 x 10 mm) containing 30 g of whole wheat grains (13% water content) infested with 20 adults of R. dominica. The treatments consisted of inoculation of A. lacunatus and A. calandrae, separately and associated, in eight replicates. Three inoculations of five adult females of the natural enemies were carried out in each petri dish at five, ten and fifteen days after the infestation of R. dominica. All treatments were stored during 60 days in environmental chamber at 30 +/- 1 degrees C, 60 +/- 5% relative humidity and 24 h scotophase. The smallest numbers of physogastric females of A. lacunatus and of adults of A. calandrae were obtained when the natural enemies were in association. The use of A. calandrae alone demonstrated a low instantaneous rate of increase (r(i)) of R. dominica and a high protection of the wheat grains. The association of A. calandrae with A. lacunatus led to the lowest number of immatures of R. dominica. These results demonstrate the importance of this interaction as a tool of for the integrated management of R. dominica in stored wheat grains.

  12. Effects of temperature and packaging types on ergosterol and Howard mold count values of tomato paste during storage.

    PubMed

    Ekinci, Raci; Kadakal, Çetin; Otağ, Mustafa

    2014-03-01

    The objective of the present study was to investigate the effects of temperature and packaging on ergosterol and Howard mold count (HMC) changes of tomato paste during storage. The other purpose of this study was to determine whether the measurement of ergosterol stability in tomato paste can be useful for the assessment of microbiological quality of tomato paste as related to the storage temperature (4, 20, 28, or 37°C) and time. Ergosterol analysis was done by using high-performance liquid chromatography. Tomato paste samples were packaged in either aseptic bags or tin boxes and stored at 4, 20, 28, or 37°C for a period of 10 months. The detection limit of ergosterol was 0.1 mg/kg. Measurements showed that packaging and storage temperatures of 28 and 37°C have a considerable influence on ergosterol and HMC changes in the product. The poor precision of the "percentage of discarded fruits" and HMC methods has increased the importance of ergosterol for the microbiological quality evaluation of tomato and tomato products. This article reports the data from what we believe to be the first survey for the influence of storage temperature and packaging material on ergosterol and HMC changes of tomato paste during storage.

  13. Principles and Practices Fostering Inclusive Excellence: Lessons from the Howard Hughes Medical Institute’s Capstone Institutions

    PubMed Central

    DiBartolo, Patricia Marten; Gregg-Jolly, Leslie; Gross, Deborah; Manduca, Cathryn A.; Iverson, Ellen; Cooke, David B.; Davis, Gregory K.; Davidson, Cameron; Hertz, Paul E.; Hibbard, Lisa; Ireland, Shubha K.; Mader, Catherine; Pai, Aditi; Raps, Shirley; Siwicki, Kathleen; Swartz, Jim E.

    2016-01-01

    Best-practices pedagogy in science, technology, engineering, and mathematics (STEM) aims for inclusive excellence that fosters student persistence. This paper describes principles of inclusivity across 11 primarily undergraduate institutions designated as Capstone Awardees in Howard Hughes Medical Institute’s (HHMI) 2012 competition. The Capstones represent a range of institutional missions, student profiles, and geographical locations. Each successfully directed activities toward persistence of STEM students, especially those from traditionally underrepresented groups, through a set of common elements: mentoring programs to build community; research experiences to strengthen scientific skill/identity; attention to quantitative skills; and outreach/bridge programs to broaden the student pool. This paper grounds these program elements in learning theory, emphasizing their essential principles with examples of how they were implemented within institutional contexts. We also describe common assessment approaches that in many cases informed programming and created traction for stakeholder buy-in. The lessons learned from our shared experiences in pursuit of inclusive excellence, including the resources housed on our companion website, can inform others’ efforts to increase access to and persistence in STEM in higher education. PMID:27562960

  14. Health-hazard evaluation report HETA-88-311-0000, Office of Disability Determination, The Howard Building, Tampa, Florida

    SciTech Connect

    Roper, P.; Lushniak, B.

    1989-01-01

    In response to a request from the Area Manager of the Office of Disability Determination, an evaluation was made of possible hazardous working conditions at the Howard Building, Tampa, Florida. Particular concern was voiced with regard to eye irritation, headaches, and fatigue with a possible connection to water leakage into the offices and pigeon nesting areas above suspended ceilings. Studies indicated no carbon-monoxide, nitrogen-dioxide, or formaldehyde concentration in the building. Carbon dioxide levels of 1000 parts per million or above were recorded which were indicative of inadequate air exchange between a building and the outdoors. An area was found along the south wall of the building with gross contamination by bird droppings and other debris. Rainwater leakage above the areas where the birds had nested washed the excrement and debris down over the office walls. As much as 2 inches of bird droppings were found on some suspended tiles. The crawl space above the first floor was observed to have health and safety hazards. Some of the symptoms were likely allergic rhinitis while others may have resulted from a lack of fresh outdoor air. Specific measures should be taken to safely remove the bird debris, prevent additional bird inhabitation, improve drainage of condensate water, and provide an outdoor air-supply system.

  15. Comprehensive clinical studies in 34 patients with molecularly defined UPD(14)pat and related conditions (Kagami-Ogata syndrome).

    PubMed

    Kagami, Masayo; Kurosawa, Kenji; Miyazaki, Osamu; Ishino, Fumitoshi; Matsuoka, Kentaro; Ogata, Tsutomu

    2015-11-01

    Paternal uniparental disomy 14 (UPD(14)pat) and epimutations and microdeletions affecting the maternally derived 14q32.2 imprinted region lead to a unique constellation of clinical features such as facial abnormalities, small bell-shaped thorax with a coat-hanger appearance of the ribs, abdominal wall defects, placentomegaly, and polyhydramnios. In this study, we performed comprehensive clinical studies in patients with UPD(14)pat (n=23), epimutations (n=5), and microdeletions (n=6), and revealed several notable findings. First, a unique facial appearance with full cheeks and a protruding philtrum and distinctive chest roentgenograms with increased coat-hanger angles to the ribs constituted the pathognomonic features from infancy through childhood. Second, birth size was well preserved, with a median birth length of ±0 SD (range, -1.7 to +3.0 SD) and a median birth weight of +2.3 SD (range, +0.1 to +8.8 SD). Third, developmental delay and/or intellectual disability was invariably present, with a median developmental/intellectual quotient of 55 (range, 29-70). Fourth, hepatoblastoma was identified in three infantile patients (8.8%), and histological examination in two patients showed a poorly differentiated embryonal hepatoblastoma with focal macrotrabecular lesions and well-differentiated hepatoblastoma, respectively. These findings suggest the necessity of an adequate support for developmental delay and periodical screening for hepatoblastoma in the affected patients, and some phenotypic overlap between UPD(14)pat and related conditions and Beckwith-Wiedemann syndrome. On the basis of our previous and present studies that have made a significant contribution to the clarification of underlying (epi)genetic factors and the definition of clinical findings, we propose the name 'Kagami-Ogata syndrome' for UPD(14)pat and related conditions.

  16. Comprehensive clinical studies in 34 patients with molecularly defined UPD(14)pat and related conditions (Kagami-Ogata syndrome).

    PubMed

    Kagami, Masayo; Kurosawa, Kenji; Miyazaki, Osamu; Ishino, Fumitoshi; Matsuoka, Kentaro; Ogata, Tsutomu

    2015-11-01

    Paternal uniparental disomy 14 (UPD(14)pat) and epimutations and microdeletions affecting the maternally derived 14q32.2 imprinted region lead to a unique constellation of clinical features such as facial abnormalities, small bell-shaped thorax with a coat-hanger appearance of the ribs, abdominal wall defects, placentomegaly, and polyhydramnios. In this study, we performed comprehensive clinical studies in patients with UPD(14)pat (n=23), epimutations (n=5), and microdeletions (n=6), and revealed several notable findings. First, a unique facial appearance with full cheeks and a protruding philtrum and distinctive chest roentgenograms with increased coat-hanger angles to the ribs constituted the pathognomonic features from infancy through childhood. Second, birth size was well preserved, with a median birth length of ±0 SD (range, -1.7 to +3.0 SD) and a median birth weight of +2.3 SD (range, +0.1 to +8.8 SD). Third, developmental delay and/or intellectual disability was invariably present, with a median developmental/intellectual quotient of 55 (range, 29-70). Fourth, hepatoblastoma was identified in three infantile patients (8.8%), and histological examination in two patients showed a poorly differentiated embryonal hepatoblastoma with focal macrotrabecular lesions and well-differentiated hepatoblastoma, respectively. These findings suggest the necessity of an adequate support for developmental delay and periodical screening for hepatoblastoma in the affected patients, and some phenotypic overlap between UPD(14)pat and related conditions and Beckwith-Wiedemann syndrome. On the basis of our previous and present studies that have made a significant contribution to the clarification of underlying (epi)genetic factors and the definition of clinical findings, we propose the name 'Kagami-Ogata syndrome' for UPD(14)pat and related conditions. PMID:25689926

  17. High-speed pre-clinical brain imaging using pulsed laser diode based photoacoustic tomography (PLD-PAT) system

    NASA Astrophysics Data System (ADS)

    Upputuri, Paul Kumar; Pramanik, Manojit

    2016-03-01

    Photoacoustic tomography (PAT) is a promising biomedical imaging modality for small animal imaging, breast cancer imaging, monitoring of vascularisation, tumor angiogenesis, blood oxygenation, total haemoglobin concentration etc. The existing PAT systems that uses Q-switched Nd:YAG and OPO nanosecond lasers have limitations in clinical applications because they are expensive, non-potable and not suitable for real-time imaging due to their low pulse repetition rate. Low-energy pulsed near-infrared diode laser which are low-cost, compact, and light-weight (<200 grams), can be used as an alternate. In this work, we present a photoacoustic tomography system with a pulsed laser diode (PLD) that can nanosecond pulses with pulse energy 1.3 mJ/pulse at ~803 nm wavelength and 7000 Hz repetition rate. The PLD is integrated inside a single-detector circular scanning geometric system. To verify the high speed imaging capabilities of the PLD-PAT system, we performed in vivo experimental results on small animal brain imaging using this system. The proposed system is portable, low-cost and can provide real-time imaging.

  18. PAT-seq: a method to study the integration of 3'-UTR dynamics with gene expression in the eukaryotic transcriptome.

    PubMed

    Harrison, Paul F; Powell, David R; Clancy, Jennifer L; Preiss, Thomas; Boag, Peter R; Traven, Ana; Seemann, Torsten; Beilharz, Traude H

    2015-08-01

    A major objective of systems biology is to quantitatively integrate multiple parameters from genome-wide measurements. To integrate gene expression with dynamics in poly(A) tail length and adenylation site, we developed a targeted next-generation sequencing approach, Poly(A)-Test RNA-sequencing. PAT-seq returns (i) digital gene expression, (ii) polyadenylation site/s, and (iii) the polyadenylation-state within and between eukaryotic transcriptomes. PAT-seq differs from previous 3' focused RNA-seq methods in that it depends strictly on 3' adenylation within total RNA samples and that the full-native poly(A) tail is included in the sequencing libraries. Here, total RNA samples from budding yeast cells were analyzed to identify the intersect between adenylation state and gene expression in response to loss of the major cytoplasmic deadenylase Ccr4. Furthermore, concordant changes to gene expression and adenylation-state were demonstrated in the classic Crabtree-Warburg metabolic shift. Because all polyadenylated RNA is interrogated by the approach, alternative adenylation sites, noncoding RNA and RNA-decay intermediates were also identified. Most important, the PAT-seq approach uses standard sequencing procedures, supports significant multiplexing, and thus replication and rigorous statistical analyses can for the first time be brought to the measure of 3'-UTR dynamics genome wide. PMID:26092945

  19. PAT-seq: a method to study the integration of 3'-UTR dynamics with gene expression in the eukaryotic transcriptome.

    PubMed

    Harrison, Paul F; Powell, David R; Clancy, Jennifer L; Preiss, Thomas; Boag, Peter R; Traven, Ana; Seemann, Torsten; Beilharz, Traude H

    2015-08-01

    A major objective of systems biology is to quantitatively integrate multiple parameters from genome-wide measurements. To integrate gene expression with dynamics in poly(A) tail length and adenylation site, we developed a targeted next-generation sequencing approach, Poly(A)-Test RNA-sequencing. PAT-seq returns (i) digital gene expression, (ii) polyadenylation site/s, and (iii) the polyadenylation-state within and between eukaryotic transcriptomes. PAT-seq differs from previous 3' focused RNA-seq methods in that it depends strictly on 3' adenylation within total RNA samples and that the full-native poly(A) tail is included in the sequencing libraries. Here, total RNA samples from budding yeast cells were analyzed to identify the intersect between adenylation state and gene expression in response to loss of the major cytoplasmic deadenylase Ccr4. Furthermore, concordant changes to gene expression and adenylation-state were demonstrated in the classic Crabtree-Warburg metabolic shift. Because all polyadenylated RNA is interrogated by the approach, alternative adenylation sites, noncoding RNA and RNA-decay intermediates were also identified. Most important, the PAT-seq approach uses standard sequencing procedures, supports significant multiplexing, and thus replication and rigorous statistical analyses can for the first time be brought to the measure of 3'-UTR dynamics genome wide.

  20. The structure of the cyanobactin domain of unknown function from PatG in the patellamide gene cluster

    SciTech Connect

    Mann, Greg; Koehnke, Jesko; Bent, Andrew F.; Graham, Rachael; Houssen, Wael; Jaspars, Marcel; Schwarz-Linek, Uli; Naismith, James H.

    2014-11-14

    The highly conserved domain of unknown function in the cyanobactin superfamily has a novel fold. The protein does not appear to bind the most plausible substrates, leaving questions as to its role. Patellamides are members of the cyanobactin family of ribosomally synthesized and post-translationally modified cyclic peptide natural products, many of which, including some patellamides, are biologically active. A detailed mechanistic understanding of the biosynthetic pathway would enable the construction of a biotechnological ‘toolkit’ to make novel analogues of patellamides that are not found in nature. All but two of the protein domains involved in patellamide biosynthesis have been characterized. The two domains of unknown function (DUFs) are homologous to each other and are found at the C-termini of the multi-domain proteins PatA and PatG. The domain sequence is found in all cyanobactin-biosynthetic pathways characterized to date, implying a functional role in cyanobactin biosynthesis. Here, the crystal structure of the PatG DUF domain is reported and its binding interactions with plausible substrates are investigated.

  1. patGPCR: A Multitemplate Approach for Improving 3D Structure Prediction of Transmembrane Helices of G-Protein-Coupled Receptors

    PubMed Central

    Wu, Hongjie; Lü, Qiang; Quan, Lijun; Qian, Peide; Xia, Xiaoyan

    2013-01-01

    The structures of the seven transmembrane helices of G-protein-coupled receptors are critically involved in many aspects of these receptors, such as receptor stability, ligand docking, and molecular function. Most of the previous multitemplate approaches have built a “super” template with very little merging of aligned fragments from different templates. Here, we present a parallelized multitemplate approach, patGPCR, to predict the 3D structures of transmembrane helices of G-protein-coupled receptors. patGPCR, which employs a bundle-packing related energy function that extends on the RosettaMem energy, parallelizes eight pipelines for transmembrane helix refinement and exchanges the optimized helix structures from multiple templates. We have investigated the performance of patGPCR on a test set containing eight determined G-protein-coupled receptors. The results indicate that patGPCR improves the TM RMSD of the predicted models by 33.64% on average against a single-template method. Compared with other homology approaches, the best models for five of the eight targets built by patGPCR had a lower TM RMSD than that obtained from SWISS-MODEL; patGPCR also showed lower average TM RMSD than single-template and multiple-template MODELLER. PMID:23554839

  2. A thermostable α-galactosidase from Lenzites elegans (Spreng.) ex Pat. MB445947: purification and properties.

    PubMed

    Sampietro, Diego; Quiroga, Emma; Sgariglia, Melina; Soberón, José; Vattuone, Marta A

    2012-08-01

    An α-galactosidase was isolated from a culture filtrate of Lenzites elegans (Spreng.) ex Pat. MB445947 grown on citric pectin as carbon source. It was purified to electrophoretic homogeneity by ammonium sulfate precipitation, gel filtration chromatography and anion-exchange chromatography. The relative molecular mass of the native purified enzyme was 158 kDa determined by gel filtration and it is a homodimer (Mr subunits = 61 kDa). The optimal temperature for enzyme activity was in the range 60-80 °C. This α-galactosidase showed a high thermostability, retaining 94 % of its activity after preincubation at 60 °C for 2 h. The optimal pH for the enzyme was 4.5 and it was stable from pH 3 to 7.5 when the preincubation took place at 60 °C for 2 h. It was active against several α-galactosides such as p-nitrophenyl-α-D-galactopyranoside, α-D-melibiose, raffinose and stachyose. The α-galactosidase is a glycoprotein with 26 % of structural sugars. Galactose was a non-competitive inhibitor with a Ki = 22 mM versus p-nitrophenyl-α-D-galactoside and 12 mM versus α-D-melibiose as substrates. Glucose was a simple competitive inhibitor with a Ki = 10 mM. Cations such as Hg(2+) and p-chloromercuribenzoate were also inhibitors of this activity, suggesting the presence of -SH groups in the active site of the enzyme. On the basis of the sequence of the N-terminus (SPDTIVLDGTNFALN) the studied α-galactosidase would be a member of glycosyl hydrolase family 36 (GH 36). Given the high optimum temperature and heat stability of L. elegans α-galactosidase, this fungus may become a useful source of α-galactosidase production for multiple applications.

  3. Photon activated therapy (PAT) using monochromatic Synchrotron x-rays and iron oxide nanoparticles in a mouse tumor model: feasibility study of PAT for the treatment of superficial malignancy

    PubMed Central

    2012-01-01

    Background X-rays are known to interact with metallic nanoparticles, producing photoelectric species as radiosensitizing effects, and have been exploited in vivo mainly with gold nanoparticles. The purpose of this study was to investigate the potential of sensitizing effect of iron oxide nanoparticles for photon activated therapy. Methods X-rays photon activated therapy (PAT) was studied by treating CT26 tumor cells and CT26 tumor-bearing mice loaded with 13-nm diameter FeO NP, and irradiating them at 7.1 keV near the Fe K-edge using synchrotron x-rays radiation. Survival of cells was determined by MTT assay, and tumor regression assay was performed for in vivo model experiment. The results of PAT treated groups were compared with x-rays alone control groups. Results A more significant reduction in viability and damage was observed in the FeO NP-treated irradiated cells, compared to the radiation alone group (p < 0.04). Injection of FeO NP (100 mg/kg) 30 min prior to irradiation elevated the tumor concentration of magnetite to 40 μg of Fe/g tissue, with a tumor-to-muscle ratio of 17.4. The group receiving FeO NP and radiation of 10 Gy showed 80% complete tumor regression (CTR) after 15–35 days and relapse-free survival for up to 6 months, compared to the control group, which showed growth retardation, resulting in 80% fatality. The group receiving radiation of 40 Gy showed 100% CTR in all cases irrespective of the presence of FeO NP, but CTR was achieved earlier in the PAT-treated group compared with the radiation alone group. Conclusions An iron oxide nanoparticle enhanced therapeutic effect with relatively low tissue concentration of iron and 10 Gy of monochromatic X-rays. Since 7.1 keV X-rays is attenuated very sharply in the tissue, FeO NP-PAT may have promise as a potent treatment option for superficial malignancies in the skin, like chest wall recurrence of breast cancer. PMID:23111059

  4. The Formation and Chronology of the PAT 91501 Impact-Melt L-Chondrite with Vesicle-Metal-Sulfide Assemblages

    NASA Technical Reports Server (NTRS)

    Benedix, G. K.; Ketcham, R. A.; Wilson, L.; McCoy, T. J.; Bogard, D. D.; Garrison, D. H.; Herzog, G. F.; Xue, S.; Klein, J.; Middleton, R.

    2007-01-01

    The L chondrite Patuxent Range (PAT) 41 91501 is an 8.5-kg unshocked, homogeneous, igneous-textured impact melt that cooled slowly compared to other meteoritic impact melts in a crater floor melt sheet or sub-crater dike. We conducted mineralogical and tomographic studies of previously unstudied mm- to cm-sized metal-sulfide-vesicle assemblages and chronologic studies of the silicate host. Metal-sulfide clasts constitute about 1 vol.%, comprise zoned taenite, troilite and pentlandite, and exhibit a consistent orientation between metal and sulfide and of metal-sulfide contacts. Vesicles make up approximately 2 vol.% and exhibit a similar orientation of long axes. Ar-39-Ar-40 measurements date the time of impact at 4.461 +/- 0.008 Gyr B.P. Cosmogenic noble gases and Be-10 and Al-2l activities suggest a pre-atmospheric radius of 40-60 cm and a cosmic ray exposure age of 25-29 Myr, similar to ages of a cluster of L chondrites. PAT 91501 dates the oldest known impact on the L chondrite parent body. The dominant vesicle-forming gas was S2 (approximately 15-20 ppm), which formed in equilibrium with impact-melted sulfides. The meteorite formed in an impact melt dike beneath a crater, as did other impact melted L chondrites, such as Chico. Cooling and solidification occurred over approximately 2 hours. During this time, approximately 90% of metal and sulfide segregated from the local melt. Remaining metal and sulfide grains oriented themselves in the local gravitational field, a feature nearly unique among meteorites. Many of these metal sulfide grains adhered to vesicles to form aggregates that may have been close to neutrally buoyant. These aggregates would have been carried upward with the residual melt, inhibiting further buoyancy-driven segregation. Although similar processes operated individually in other chondritic impact melts, their interaction produced the unique assemblage observed in PAT 91501.

  5. Ways of knowing: Howard Stein's border-crossing use of poetry to interrogate clinical medicine, medical education, and health care organizations.

    PubMed

    Shapiro, Johanna

    2016-09-01

    This article explores how medical anthropologist Howard Stein's poetry and his unique practice of sharing this poetry with the patients, physicians, and administrators who inspired it create ways of knowing that are at once revelatory and emancipatory. Stein's writing shows readers that poetry can be considered as a form of data and as a method of investigation into the processes of the human soul. Furthermore, it represents a kind of intervention that invites health professional readers toward connection, bridge building, and solidarity with their patients and with one another. (PsycINFO Database Record PMID:27632547

  6. Helicopter AFMAG (ZTEM) EM and magnetic results over sedimentary exhalative (SEDEX) lead-zinc deposits at Howard's Pass in Selwyn Basin, Yukon

    NASA Astrophysics Data System (ADS)

    Legault, Jean M.; Latrous, Ali; Zhao, Shengkai; Bournas, Nasreddine; Plastow, Geoffrey C.; Xue, Gabriel Guang

    2016-01-01

    In 2008, a regional scale 24,675 line-km survey covering a 25,000 km2 area (1 km line spacing) was flown in the Selwyn Basin. The survey footprint straddles east-central Yukon and overlaps into the western North-west Territories. In March 2013, Yukon Geological Survey purchased the survey data and, in November 2013, released the data publicly. The Selwyn Basin area is prospective for sedimentary exhalative (SEDEX)-style Pb-Zn-Ag mineralisation and the z-axis tipper electromagnetic (ZTEM) survey data provide insights into regional structures and plutons in the region. The survey overflew the Howard's Pass SEDEX deposits at the south-eastern edge of the Selwyn Basin survey area that hosts a ~250 million tonne resource with ~4.5% Zn and ~1.5% Pb. Airborne geophysics has not been extensively used in SEDEX exploration of the Selwyn Basin and the ZTEM survey is one of the few publicly available airborne audio-frequency magnetic (AFMAG) EM-magnetic datasets that offer the opportunity to study the deposit response at Howard's Pass in close detail. Rock physical properties indicate that the lowest resistivities are associated with the Road River Group that contains the Pb-Zn mineralised horizon at Howard's Pass, but also include graphitic shales in the same formation. Major NW-SE to ESE and minor NNW-SSE linear conductive trends correlate with known regional geologic, structural and inferred mineral trends that were previously not visible in magnetic results. At the deposit scale, a thin NW-SE trending conductive lineament extends along the > 37-km-long `Zinc Corridor' horizon at Howard's Pass, but must include both the Pb-Zn sulphide mineralisation deposit horizon as well as the surrounding graphitic black shales. 2D and 3D ZTEM inversions reveal zones of enhanced conductivity along strike and at depth that appear to correlate with the clustering of Pb-Zn deposits, which had not been previously noticed.

  7. A Scintillation Counter System Design To Detect Antiproton Annihilation using the High Performance Antiproton Trap(HiPAT)

    NASA Technical Reports Server (NTRS)

    Martin, James J.; Lewis, Raymond A.; Stanojev, Boris

    2003-01-01

    The High Performance Antiproton Trap (HiPAT), a system designed to hold up to l0(exp 12) charge particles with a storage half-life of approximately 18 days, is a tool to support basic antimatter research. NASA's interest stems from the energy density represented by the annihilation of matter with antimatter, 10(exp 2)MJ/g. The HiPAT is configured with a Penning-Malmberg style electromagnetic confinement region with field strengths up to 4 Tesla, and 20kV. To date a series of normal matter experiments, using positive and negative ions, have been performed evaluating the designs performance prior to operations with antiprotons. The primary methods of detecting and monitoring stored normal matter ions and antiprotons within the trap includes a destructive extraction technique that makes use of a micro channel plate (MCP) device and a non-destractive radio frequency scheme tuned to key particle frequencies. However, an independent means of detecting stored antiprotons is possible by making use of the actual annihilation products as a unique indicator. The immediate yield of the annihilation event includes photons and pie mesons, emanating spherically from the point of annihilation. To "count" these events, a hardware system of scintillators, discriminators, coincident meters and multi channel scalars (MCS) have been configured to surround much of the HiPAT. Signal coincidence with voting logic is an essential part of this system, necessary to weed out the single cosmic ray events from the multi-particle annihilation shower. This system can be operated in a variety of modes accommodating various conditions. The first is a low-speed sampling interval that monitors the background loss or "evaporation" rate of antiprotons held in the trap during long storage periods; provides an independent method of validating particle lifetimes. The second is a high-speed sample rate accumulating information on a microseconds time-scale; useful when trapped antiparticles are extracted

  8. Discharge diversion in the Patía River delta, the Colombian Pacific: Geomorphic and ecological consequences for mangrove ecosystems

    NASA Astrophysics Data System (ADS)

    Restrepo, Juan D.; Cantera, Jaime R.

    2013-10-01

    In the Patía River delta, the best-developed delta on the western margin of South America, a major water diversion started in 1972. The diversion of the Patía flow to the Sanquianga River, the latter a small stream draining internal lakes from the Pacific lowlands, shifted the active delta plain from the south to the north and changed the northern estuarine system into an active delta plain. The Sanquianga Mangrove National Park, a mangrove reserve measuring 800 km2, lies in this former estuary, where major hydrologic and sedimentation changes are occurring. Overall, major environmental consequences of this discharge diversion in terms of geomorphic changes along distributary channels and ecological impacts on mangrove ecosystems are evidenced by: (1) distributary channel accretion by operating processes such as sedimentation, overbank flow, increasing width of levees, sedimentation in crevasses, interdistributary channel fill, and colonization of pioneer mangrove; (2) freshening conditions in the Sanquianga distributary channel, a hydrologic change that has shifted the upper estuarine region (salinity <1%) downstream; (3) downstream advance of freshwater vegetation, which is invading channel banks in the lower and mixing estuarine zones; (4) die-off of approximately 5200 ha of mangrove near the delta apex at Bocas de Satinga, where the highest sediment accumulation rates occur; and (5) recurrent periods of mangrove defoliation due to a worm plague. Further analyses indicate strong mangrove erosion along transgressive barrier islands on the former delta plain. Here tectonic-induced subsidence, relative sea-level rise, and sediment starving conditions due to the channel diversion, are the main causes of the observed retreating conditions of mangrove communities. Our data also indicate that the Patía River has the highest sediment load (27 × 106 t yr-1) and basin-wide sediment yield (1500 t km-2 yr-1) on the west coast of South America. Erosion rates from the Pat

  9. Review of the High Performance Antiproton Trap (HiPAT) Experiment at the Marshall Space Flight Center

    NASA Technical Reports Server (NTRS)

    Pearson, J. B.; Sims, Herb; Martin, James; Chakrabarti, Suman; Lewis, Raymond; Fant, Wallace

    2003-01-01

    The significant energy density of matter-antimatter annihilation is attractive to the designers of future space propulsion systems, with the potential to offer a highly compact source of power. Many propulsion concepts exist that could take advantage of matter-antimatter reactions, and current antiproton production rates are sufficient to support basic proof-of-principle evaluation of technology associated with antimatter- derived propulsion. One enabling technology for such experiments is portable storage of low energy antiprotons, allowing antiprotons to be trapped, stored, and transported for use at an experimental facility. To address this need, the Marshall Space Flight Center's Propulsion Research Center is developing a storage system referred to as the High Performance Antiproton Trap (HiPAT) with a design goal of containing 10(exp 12) particles for up to 18 days. The HiPAT makes use of an electromagnetic system (Penning- Malmberg design) consisting of a 4 Telsa superconductor, high voltage electrode structure, radio frequency (RF) network, and ultra high vacuum system. To evaluate the system normal matter sources (both electron guns and ion sources) are used to generate charged particles. The electron beams ionize gas within the trapping region producing ions in situ, whereas the ion sources produce the particles external to the trapping region and required dynamic capture. A wide range of experiments has been performed examining factors such as ion storage lifetimes, effect of RF energy on storage lifetime, and ability to routinely perform dynamic ion capture. Current efforts have been focused on improving the FW rotating wall system to permit longer storage times and non-destructive diagnostics of stored ions. Typical particle detection is performed by extracting trapped ions from HiPAT and destructively colliding them with a micro-channel plate detector (providing number and energy information). This improved RF system has been used to detect various

  10. Howard, Trudy Mulcahy

    ERIC Educational Resources Information Center

    Momentum, 1972

    1972-01-01

    The mystique says that the women will always be available whenever the Church needs them, but unless we wipe the stardust from our eyes, we will be faced with an alarming shortage of capable candidates for the few archdiocesan positions that are finally carved out for women to serve. (Author/CB)

  11. The mRNA decay factor PAT1 functions in a pathway including MAP kinase 4 and immune receptor SUMM2

    PubMed Central

    Roux, Milena Edna; Rasmussen, Magnus Wohlfahrt; Palma, Kristoffer; Lolle, Signe; Regué, Àngels Mateu; Bethke, Gerit; Glazebrook, Jane; Zhang, Weiping; Sieburth, Leslie; Larsen, Martin R; Mundy, John; Petersen, Morten

    2015-01-01

    Multi-layered defense responses are activated in plants upon recognition of invading pathogens. Transmembrane receptors recognize conserved pathogen-associated molecular patterns (PAMPs) and activate MAP kinase cascades, which regulate changes in gene expression to produce appropriate immune responses. For example, Arabidopsis MAP kinase 4 (MPK4) regulates the expression of a subset of defense genes via at least one WRKY transcription factor. We report here that MPK4 is found in complexes in vivo with PAT1, a component of the mRNA decapping machinery. PAT1 is also phosphorylated by MPK4 and, upon flagellin PAMP treatment, PAT1 accumulates and localizes to cytoplasmic processing (P) bodies which are sites for mRNA decay. Pat1 mutants exhibit dwarfism and de-repressed immunity dependent on the immune receptor SUMM2. Since mRNA decapping is a critical step in mRNA turnover, linking MPK4 to mRNA decay via PAT1 provides another mechanism by which MPK4 may rapidly instigate immune responses. PMID:25603932

  12. Safety evaluation of the phosphinothricin acetyltransferase proteins encoded by the pat and bar sequences that confer tolerance to glufosinate-ammonium herbicide in transgenic plants.

    PubMed

    Hérouet, Corinne; Esdaile, David J; Mallyon, Bryan A; Debruyne, Eric; Schulz, Arno; Currier, Thomas; Hendrickx, Koen; van der Klis, Robert-Jan; Rouan, Dominique

    2005-03-01

    Transgenic plant varieties, which are tolerant to glufosinate-ammonium, were developed. The herbicide tolerance is based upon the presence of either the bar or the pat gene, which encode for two homologous phosphinothricin acetyltransferases (PAT), in the plant genome. Based on both a review of published literature and experimental studies, the safety assessment reviews the first step of a two-step-approach for the evaluation of the safety of the proteins expressed in plants. It can be used to support the safety of food or feed products derived from any crop that contains and expresses these PAT proteins. The safety evaluation supports the conclusion that the genes and the donor microorganisms (Streptomyces) are innocuous. The PAT enzymes are highly specific and do not possess the characteristics associated with food toxins or allergens, i.e., they have no sequence homology with any known allergens or toxins, they have no N-glycosylation sites, they are rapidly degraded in gastric and intestinal fluids, and they are devoid of adverse effects in mice after intravenous administration at a high dose level. In conclusion, there is a reasonable certainty of no harm resulting from the inclusion of the PAT proteins in human food or in animal feed.

  13. Small GTPase CDC-42 promotes apoptotic cell corpse clearance in response to PAT-2 and CED-1 in C. elegans.

    PubMed

    Neukomm, L J; Zeng, S; Frei, A P; Huegli, P A; Hengartner, M O

    2014-06-01

    The rapid clearance of dying cells is important for the well-being of multicellular organisms. In C. elegans, cell corpse removal is mainly mediated by three parallel engulfment signaling cascades. These pathways include two small GTPases, MIG-2/RhoG and CED-10/Rac1. Here we present the identification and characterization of CDC-42 as a third GTPase involved in the regulation of cell corpse clearance. Genetic analyses performed by both loss of cdc-42 function and cdc-42 overexpression place cdc-42 in parallel to the ced-2/5/12 signaling module, in parallel to or upstream of the ced-10 module, and downstream of the ced-1/6/7 module. CDC-42 accumulates in engulfing cells at membranes surrounding apoptotic corpses. The formation of such halos depends on the integrins PAT-2/PAT-3, UNC-112 and the GEF protein UIG-1, but not on the canonical ced-1/6/7 or ced-2/5/12 signaling modules. Together, our results suggest that the small GTPase CDC-42 regulates apoptotic cell engulfment possibly upstream of the canonical Rac GTPase CED-10, by polarizing the engulfing cell toward the apoptotic corpse in response to integrin signaling and ced-1/6/7 signaling in C. elegans.

  14. Structural basis for selective recognition of acyl chains by the membrane-associated acyltransferase PatA

    PubMed Central

    Albesa-Jové, David; Svetlíková, Zuzana; Tersa, Montse; Sancho-Vaello, Enea; Carreras-González, Ana; Bonnet, Pascal; Arrasate, Pedro; Eguskiza, Ander; Angala, Shiva K.; Cifuente, Javier O.; Korduláková, Jana; Jackson, Mary; Mikušová, Katarína; Guerin, Marcelo E.

    2016-01-01

    The biosynthesis of phospholipids and glycolipids are critical pathways for virtually all cell membranes. PatA is an essential membrane associated acyltransferase involved in the biosynthesis of mycobacterial phosphatidyl-myo-inositol mannosides (PIMs). The enzyme transfers a palmitoyl moiety from palmitoyl–CoA to the 6-position of the mannose ring linked to 2-position of inositol in PIM1/PIM2. We report here the crystal structures of PatA from Mycobacterium smegmatis in the presence of its naturally occurring acyl donor palmitate and a nonhydrolyzable palmitoyl–CoA analog. The structures reveal an α/β architecture, with the acyl chain deeply buried into a hydrophobic pocket that runs perpendicular to a long groove where the active site is located. Enzyme catalysis is mediated by an unprecedented charge relay system, which markedly diverges from the canonical HX4D motif. Our studies establish the mechanistic basis of substrate/membrane recognition and catalysis for an important family of acyltransferases, providing exciting possibilities for inhibitor design. PMID:26965057

  15. ASC-1, PAT2, and P2RX5 are cell surface markers for white, beige, and brown adipocytes.

    PubMed

    Ussar, Siegfried; Lee, Kevin Y; Dankel, Simon N; Boucher, Jeremie; Haering, Max-Felix; Kleinridders, Andre; Thomou, Thomas; Xue, Ruidan; Macotela, Yazmin; Cypess, Aaron M; Tseng, Yu-Hua; Mellgren, Gunnar; Kahn, C Ronald

    2014-07-30

    White, beige, and brown adipocytes are developmentally and functionally distinct but often occur mixed together within individual depots. To target white, beige, and brown adipocytes for diagnostic or therapeutic purposes, a better understanding of the cell surface properties of these cell types is essential. Using a combination of in silico, in vitro, and in vivo methods, we have identified three new cell surface markers of adipose cell types. The amino acid transporter ASC-1 is a white adipocyte-specific cell surface protein, with little or no expression in brown adipocytes, whereas the amino acid transporter PAT2 and the purinergic receptor P2RX5 are cell surface markers expressed in classical brown and beige adipocytes in mice. These markers also selectively mark brown/beige and white adipocytes in human tissue. Thus, ASC-1, PAT2, and P2RX5 are membrane surface proteins that may serve as tools to identify and target white and brown/beige adipocytes for therapeutic purposes.

  16. Mass-spectrometry analysis of histone post-translational modifications in pathology tissue using the PAT-H-MS approach.

    PubMed

    Noberini, Roberta; Pruneri, Giancarlo; Minucci, Saverio; Bonaldi, Tiziana

    2016-06-01

    Aberrant histone post-translational modifications (hPTMs) have been implicated with various pathologies, including cancer, and may represent useful epigenetic biomarkers. The data described here provide a mass spectrometry-based quantitative analysis of hPTMs from formalin-fixed paraffin-embedded (FFPE) tissues, from which histones were extracted through the recently developed PAT-H-MS method. First, we analyzed FFPE samples from mouse spleen and liver or human breast cancer up to six years old, together with their corresponding fresh frozen tissue. We then combined the PAT-H-MS approach with a histone-focused version of the super-SILAC strategy-using a mix of histones from four breast cancer cell lines as a spike-in standard- to accurately quantify hPTMs from breast cancer specimens belonging to different subtypes. The data, which are associated with a recent publication (Pathology tissue-quantitative mass spectrometry analysis to profile histone post-translational modification patterns in patient samples (Noberini, 2015) [1]), are deposited at the ProteomeXchange Consortium via the PRIDE partner repository with the dataset identifier PXD002669. PMID:27408908

  17. Asc-1, PAT2 and P2RX5 are novel cell surface markers for white, beige and brown adipocytes

    PubMed Central

    Ussar, Siegfried; Lee, Kevin Y.; Dankel, Simon N.; Boucher, Jeremie; Haering, Max-Felix; Kleinridders, Andre; Thomou, Thomas; Xue, Ruidan; Macotela, Yazmin; Cypess, Aaron M.; Tseng, Yu-Hua; Mellgren, Gunnar; Kahn, C. Ronald

    2015-01-01

    White, beige and brown adipocytes are developmentally and functionally distinct but often occur mixed together within individual depots. To target white, beige and brown adipocytes for diagnostic or therapeutic purposes, a better understanding of the cell surface properties of these cell types is essential. Using a combination of in silico, in vitro and in vivo methods, we have identified three new cell surface markers of adipose cell types. The amino acid transporter Asc-1 is a white adipocyte-specific cell surface protein, with little or no expression in brown adipocytes, whereas the amino acid transporter PAT2 and the purinergic receptor P2RX5 are cell surface markers expressed in classical brown and beige adipocytes in mice. These markers also selectively mark brown/beige and white adipocytes in human tissue. Thus, Asc-1, PAT2 and P2RX5 are membrane surface proteins that may serve as tools to identify and target white and brown/beige adipocytes for therapeutic purposes. PMID:25080478

  18. Structural basis for selective recognition of acyl chains by the membrane-associated acyltransferase PatA.

    PubMed

    Albesa-Jové, David; Svetlíková, Zuzana; Tersa, Montse; Sancho-Vaello, Enea; Carreras-González, Ana; Bonnet, Pascal; Arrasate, Pedro; Eguskiza, Ander; Angala, Shiva K; Cifuente, Javier O; Korduláková, Jana; Jackson, Mary; Mikušová, Katarína; Guerin, Marcelo E

    2016-01-01

    The biosynthesis of phospholipids and glycolipids are critical pathways for virtually all cell membranes. PatA is an essential membrane associated acyltransferase involved in the biosynthesis of mycobacterial phosphatidyl-myo-inositol mannosides (PIMs). The enzyme transfers a palmitoyl moiety from palmitoyl-CoA to the 6-position of the mannose ring linked to 2-position of inositol in PIM1/PIM2. We report here the crystal structures of PatA from Mycobacterium smegmatis in the presence of its naturally occurring acyl donor palmitate and a nonhydrolyzable palmitoyl-CoA analog. The structures reveal an α/β architecture, with the acyl chain deeply buried into a hydrophobic pocket that runs perpendicular to a long groove where the active site is located. Enzyme catalysis is mediated by an unprecedented charge relay system, which markedly diverges from the canonical HX4D motif. Our studies establish the mechanistic basis of substrate/membrane recognition and catalysis for an important family of acyltransferases, providing exciting possibilities for inhibitor design. PMID:26965057

  19. Strategic funding priorities in the pharmaceutical sciences allied to Quality by Design (QbD) and Process Analytical Technology (PAT).

    PubMed

    Aksu, Buket; De Beer, Thomas; Folestad, Staffan; Ketolainen, Jarkko; Lindén, Hans; Lopes, Joao Almeida; de Matas, Marcel; Oostra, Wim; Rantanen, Jukka; Weimer, Marco

    2012-09-29

    Substantial changes in Pharmaceutical R&D strategy are required to address existing issues of low productivity, imminent patent expirations and pressures on pricing. Moves towards personalized healthcare and increasing diversity in the nature of portfolios including the rise of biopharmaceuticals however have the potential to provide considerable challenges to the establishment of cost effective and robust supply chains. To guarantee product quality and surety of supply for essential medicines it is necessary that manufacturing science keeps pace with advances in pharmaceutical R&D. In this position paper, the EUFEPS QbD and PAT Sciences network make recommendations that European industry, academia and health agencies focus attention on delivering step changes in science and technology in a number of key themes. These subject areas, all underpinned by the sciences allied to QbD and PAT, include product design and development for personalized healthcare, continuous-processing in pharmaceutical product manufacture, quantitative quality risk assessment for pharmaceutical development including life cycle management and the downstream processing of biopharmaceutical products. Plans are being established to gain commitment for inclusion of these themes into future funding priorities for the Innovative Medicines Initiative (IMI).

  20. Human induced discharge diversion in a tropical delta and its environmental implications: The Patía River, Colombia

    NASA Astrophysics Data System (ADS)

    Restrepo, Juan D.; Kettner, Albert

    2012-03-01

    SummaryThe Patía River, the number one in terms of sediment yield ˜1500 t km-2 yr-1 draining the western South America, has the most extensive and well developed delta on the Pacific coast, measuring 1700 km2. During the Holocene, nature forced the Patía delta to the south; however, a major water diversion, starting in 1972, diverted the Patía flow to the Sanguianga River, the latter, a small stream draining internal lakes from the Pacific lowlands. This human induced discharge diversion shifted the active delta plain back to the north and changed the northern estuarine system into an active delta plain. Overall, major environmental consequences of this discharge diversion in terms of morphological changes along the delta coast and distributary channels, are evidenced by: (1) coastal retreat along the abandoned delta lobe; 63% of the southern shoreline is retreating at maximum rates of 7 m yr-1, with a corresponding coastal land loss of 106 m yr-1; (2) transgressive barrier islands with exposed peat soils in the surf zone; (3) abandonment of former active distributaries in the southern delta plain with associated closing of inlets and formation of ebb tidal deltas; (4) breaching events on barrier islands; and (5) distributary channel accretion in the northern delta plain by morphological processes such as sedimentation (also in crevasses), overbank flow, increasing width of levees, interdistributary channel fill, and colonization of pioneer mangrove. The Sanguianga Mangrove National Park (SMNP), the largest mangrove reserve in Colombia, measuring 800 km2, lies in this former estuary, where major hydrologic and sedimentation changes are occurring. Observed environmental changes in the SMNP, include (1) seaward advance of the sub-aqueous delta front at the Sanquianga inlet evidenced by an increase in tidal flat area from 5.4 Mm2 in 1986 to 14 Mm2 in 2001; (2) freshening conditions in the Sanguianga distributary channel, a hydrologic change that has shifted the

  1. The distinct roles of anion transporters Slc26a3 (DRA) and Slc26a6 (PAT-1) in fluid and electrolyte absorption in the murine small intestine.

    PubMed

    Xia, Weiliang; Yu, Qin; Riederer, Brigitte; Singh, Anurag Kumar; Engelhardt, Regina; Yeruva, Sunil; Song, Penghong; Tian, De-An; Soleiman, Manoocher; Seidler, Ursula

    2014-08-01

    The mixing of gastric and pancreatic juice subjects the jejunum to unique ionic conditions with high luminal CO2 tension and HCO3 − concentration. We investigated the role of the small intestinal apical anion exchangers PAT-1 (Slc26a6) and DRA (Slc26a3) in basal and CO2/HCO3 −-stimulated jejunal fluid absorption. Single pass perfusion of jejunal segments was performed in anaesthetised wild type (WT) as well as in mice deficient in DRA, PAT-1, Na+/H+ exchanger 3 (NHE3) or NHE2, and in carbonic anhydrase II (CAII). Unbuffered saline (pH 7.4) perfusion of WT jejunum resulted in fluid absorption and acidification of the effluent. DRA-deficient jejunum absorbed less fluid than WT, and acidified the effluent more strongly, consistent with its action as a Cl−/HCO3 − exchanger. PAT-1-deficient jejunum also absorbed less fluid but resulted in less effluent acidification. Switching the luminal solution to a 5 % CO2/HCO3 − buffered solution (pH 7.4), resulted in a decrease in jejunal enterocyte pHi in all genotypes, an increase in luminal surface pH and a strong increase in fluid absorption in a PAT-1- and NHE3- but not DRA-, CAII, or NHE2-dependent fashion. Even in the absence of luminal Cl−, luminal CO2/HCO3 − augmented fluid absorption in WT, CAII, NHE2- or DRA-deficient, but not in PAT-1- or NHE3-deficient mice, indicating the likelihood that PAT-1 serves to import HCO3 − and NHE3 serves to import Na+ under these circumstances. The results suggest that PAT-1 plays an important role in jejunal Na+HCO3 – reabsorption, while DRA absorbs Cl− and exports HCO3 − in a partly CAII-dependent fashion. Both PAT-1 and DRA significantly contribute to intestinal fluid absorption and enterocyte acid/base balance but are activated by different ion gradients.

  2. The formation and chronology of the PAT 91501 impact-melt L chondrite with vesicle metal sulfide assemblages

    NASA Astrophysics Data System (ADS)

    Benedix, G. K.; Ketcham, R. A.; Wilson, L.; McCoy, T. J.; Bogard, D. D.; Garrison, D. H.; Herzog, G. F.; Xue, S.; Klein, J.; Middleton, R.

    2008-05-01

    The L chondrite Patuxent Range (PAT) 91501 is an 8.5-kg unshocked, homogeneous, igneous-textured impact melt that cooled slowly compared to other meteoritic impact melts in a crater floor melt sheet or sub-crater dike [Mittlefehldt D. W. and Lindstrom M. M. (2001) Petrology and geochemistry of Patuxent Range 91501 and Lewis Cliff 88663. Meteoritics Planet. Sci. 36, 439-457]. We conducted mineralogical and tomographic studies of previously unstudied mm- to cm-sized metal-sulfide-vesicle assemblages and chronologic studies of the silicate host. Metal-sulfide clasts constitute about 1 vol.%, comprise zoned taenite, troilite, and pentlandite, and exhibit a consistent orientation between metal and sulfide and of metal-sulfide contacts. Vesicles make up ˜2 vol.% and exhibit a similar orientation of long axes. 39Ar- 40Ar measurements probably date the time of impact at 4.461 ± 0.008 Gyr B.P. Cosmogenic noble gases and 10Be and 26Al activities suggest a pre-atmospheric radius of 40-60 cm and a cosmic ray exposure age of 25-29 Myr, similar to ages of a cluster of L chondrites. PAT 91501 dates the oldest known impact on the L chondrite parent body. The dominant vesicle-forming gas was S 2 (˜15-20 ppm), which formed in equilibrium with impact-melted sulfides. The meteorite formed in an impact melt dike beneath a crater, as did other impact melted L chondrites, such as Chico. Cooling and solidification occurred over ˜2 h. During this time, ˜90% of metal and sulfide segregated from the local melt. Remaining metal and sulfide grains oriented themselves in the local gravitational field, a feature nearly unique among meteorites. Many of these metal-sulfide grains adhered to vesicles to form aggregates that may have been close to neutrally buoyant. These aggregates would have been carried upward with the residual melt, inhibiting further buoyancy-driven segregation. Although similar processes operated individually in other chondritic impact melts, their interaction produced

  3. Hybrid modeling for quality by design and PAT-benefits and challenges of applications in biopharmaceutical industry.

    PubMed

    von Stosch, Moritz; Davy, Steven; Francois, Kjell; Galvanauskas, Vytautas; Hamelink, Jan-Martijn; Luebbert, Andreas; Mayer, Martin; Oliveira, Rui; O'Kennedy, Ronan; Rice, Paul; Glassey, Jarka

    2014-06-01

    This report highlights the drivers, challenges, and enablers of the hybrid modeling applications in biopharmaceutical industry. It is a summary of an expert panel discussion of European academics and industrialists with relevant scientific and engineering backgrounds. Hybrid modeling is viewed in its broader sense, namely as the integration of different knowledge sources in form of parametric and nonparametric models into a hybrid semi-parametric model, for instance the integration of fundamental and data-driven models. A brief description of the current state-of-the-art and industrial uptake of the methodology is provided. The report concludes with a number of recommendations to facilitate further developments and a wider industrial application of this modeling approach. These recommendations are limited to further exploiting the benefits of this methodology within process analytical technology (PAT) applications in biopharmaceutical industry.

  4. Patent information analysis methods and their effective use : A study through activities of PAT-LIST Research Workshop adviser

    NASA Astrophysics Data System (ADS)

    Nakamura, Sakae

    For effective use of technical information, various analytical tools and methods (e.g., patent map analysis) have been proposed. It was against this background that the “PAT-LIST Research Workshop” (supported by Raytec Co., Ltd.) was established in 2006. This article discusses, as an example, some actual research subject that the author as an adviser to the forum has studied through our activities in the past six years, especially the subject for 2010 (unveiling intellectual property strategies of specified enterprises from technical information analysis results). Practically useful analysis methods will be proposed showing some points of notes in analysis about the methods. What is also introduced is macroanalysis using text mining tools and the significance of controlled technical classification in a problem/solution map for determining critical fields.

  5. Strategic framework for education and training in Quality by Design (QbD) and process analytical technology (PAT).

    PubMed

    de Matas, Marcel; De Beer, Thomas; Folestad, Staffan; Ketolainen, Jarkko; Lindén, Hans; Lopes, João Almeida; Oostra, Wim; Weimer, Marco; Öhrngren, Per; Rantanen, Jukka

    2016-07-30

    The regulatory and technical landscape of the pharmaceutical field is rapidly evolving from one focused predominantly on development of small molecules, using well established manufacturing technologies towards an environment in which biologicals and complex modalities are being developed using advanced science and technology coupled with the application of modern Quality by Design (QbD) principles. In order that Europe keeps pace with these changes and sustains its position as major player in the development and commercialization of medicines, it is essential that measures are put in place to maintain a highly skilled workforce. A number of challenges however exist to equipping academic, industrial and health agency staff with the requisite knowledge, skills and experience to develop the next generation of medicines. In this regard, the EUFEPS QbD and PAT Sciences Network has proposed a structured framework for education, training and continued professional development, which comprises a number of pillars covering the fundamental principles of modern pharmaceutical development including the underpinning aspects of science, engineering and technology innovation. The framework is not prescriptive and is not aimed at describing specific course content in detail. It should however be used as a point of reference for those institutions delivering pharmaceutical based educational courses, to ensure that the necessary skills, knowledge and experience for successful pharmaceutical development are maintained. A positive start has been made and a number of examples of formal higher education courses and short training programs containing elements of this framework have been described. The ultimate vision for this framework however, is to see widespread adoption and proliferation of this curriculum with it forming the backbone of QbD and PAT science based skills development. PMID:27112991

  6. A Process Analytical Technology (PAT) approach to control a new API manufacturing process: development, validation and implementation.

    PubMed

    Schaefer, Cédric; Clicq, David; Lecomte, Clémence; Merschaert, Alain; Norrant, Edith; Fotiadu, Frédéric

    2014-03-01

    Pharmaceutical companies are progressively adopting and introducing Process Analytical Technology (PAT) and Quality-by-Design (QbD) concepts promoted by the regulatory agencies, aiming the building of the quality directly into the product by combining thorough scientific understanding and quality risk management. An analytical method based on near infrared (NIR) spectroscopy was developed as a PAT tool to control on-line an API (active pharmaceutical ingredient) manufacturing crystallization step during which the API and residual solvent contents need to be precisely determined to reach the predefined seeding point. An original methodology based on the QbD principles was designed to conduct the development and validation of the NIR method and to ensure that it is fitted for its intended use. On this basis, Partial least squares (PLS) models were developed and optimized using chemometrics methods. The method was fully validated according to the ICH Q2(R1) guideline and using the accuracy profile approach. The dosing ranges were evaluated to 9.0-12.0% w/w for the API and 0.18-1.50% w/w for the residual methanol. As by nature the variability of the sampling method and the reference method are included in the variability obtained for the NIR method during the validation phase, a real-time process monitoring exercise was performed to prove its fit for purpose. The implementation of this in-process control (IPC) method on the industrial plant from the launch of the new API synthesis process will enable automatic control of the final crystallization step in order to ensure a predefined quality level of the API. In addition, several valuable benefits are expected including reduction of the process time, suppression of a rather difficult sampling and tedious off-line analyses.

  7. A Process Analytical Technology (PAT) approach to control a new API manufacturing process: development, validation and implementation.

    PubMed

    Schaefer, Cédric; Clicq, David; Lecomte, Clémence; Merschaert, Alain; Norrant, Edith; Fotiadu, Frédéric

    2014-03-01

    Pharmaceutical companies are progressively adopting and introducing Process Analytical Technology (PAT) and Quality-by-Design (QbD) concepts promoted by the regulatory agencies, aiming the building of the quality directly into the product by combining thorough scientific understanding and quality risk management. An analytical method based on near infrared (NIR) spectroscopy was developed as a PAT tool to control on-line an API (active pharmaceutical ingredient) manufacturing crystallization step during which the API and residual solvent contents need to be precisely determined to reach the predefined seeding point. An original methodology based on the QbD principles was designed to conduct the development and validation of the NIR method and to ensure that it is fitted for its intended use. On this basis, Partial least squares (PLS) models were developed and optimized using chemometrics methods. The method was fully validated according to the ICH Q2(R1) guideline and using the accuracy profile approach. The dosing ranges were evaluated to 9.0-12.0% w/w for the API and 0.18-1.50% w/w for the residual methanol. As by nature the variability of the sampling method and the reference method are included in the variability obtained for the NIR method during the validation phase, a real-time process monitoring exercise was performed to prove its fit for purpose. The implementation of this in-process control (IPC) method on the industrial plant from the launch of the new API synthesis process will enable automatic control of the final crystallization step in order to ensure a predefined quality level of the API. In addition, several valuable benefits are expected including reduction of the process time, suppression of a rather difficult sampling and tedious off-line analyses. PMID:24468350

  8. A double-focus collimator system for full PAT performance testing of inter-satellite laser communication terminals

    NASA Astrophysics Data System (ADS)

    Wang, Lijuan; Luan, Zhu; Sun, Jianfeng; Zhou, Yu; Liu, De'an; Liu, Liren

    2006-08-01

    A laser collimator is necessary for the testing and verification of the PAT performance of inter-satellite laser communication terminals. However, the terminals mostly have a large field of view for the acquisition and a high angular accuracy for the fine tracking needed to be examined. A single collimator has the conflict to reach at both a large field of view and a fine resolution. To compromise, a double-focus laser collimator is proposed. The collimator is mainly composed of a primary lens, a beam splitter, a secondary lens and some reflectors. The primary lens with a 9.9m focal length directly forms the long focal length arm of the collimator. The combination of the primary lens and the secondary lens has a new focal length of 1.3m and constructs the short focal length arm of the collimator. With two CMOS imaging sensors, the collimator can realize a 1.1mrad field of view with a <1μrad resolution in the focal plane of the long focal length arm and a 8.3mrad field of view with a 8.2μrad resolution in the focal plane of the short focal length arm. In combination with a coarse beam scanner (+/-15°) and a fine beam scanner (1mrad) to simulate the mutual angular movement between two satellites, the united system is capable to test the full PAT performance of inter-satellite laser communication terminals. The optical layouts of the collimator and two detecting units are illustrated. The optical design of the collimator is detailed. The mechanical design of the collimator is given.

  9. Decay of Fecal Indicator Bacterial Populations and Bovine-Associated Source-Tracking Markers in Freshly Deposited Cow Pats

    PubMed Central

    Oladeinde, Adelumola; Bohrmann, Thomas; Wong, Kelvin; Purucker, S. T.; Bradshaw, Ken; Brown, Reid; Snyder, Blake

    2014-01-01

    Understanding the survival of fecal indicator bacteria (FIB) and microbial source-tracking (MST) markers is critical to developing pathogen fate and transport models. Although pathogen survival in water microcosms and manure-amended soils is well documented, little is known about their survival in intact cow pats deposited on pastures. We conducted a study to determine decay rates of fecal indicator bacteria (Escherichia coli and enterococci) and bovine-associated MST markers (CowM3, Rum-2-bac, and GenBac) in 18 freshly deposited cattle feces from three farms in northern Georgia. Samples were randomly assigned to shaded or unshaded treatment in order to determine the effects of sunlight, moisture, and temperature on decay rates. A general linear model (GLM) framework was used to determine decay rates. Shading significantly decreased the decay rate of the E. coli population (P < 0.0001), with a rate of −0.176 day−1 for the shaded treatment and −0.297 day−1 for the unshaded treatment. Shading had no significant effect on decay rates of enterococci, CowM3, Rum-2-bac, and GenBac (P > 0.05). In addition, E. coli populations showed a significant growth rate (0.881 day−1) in the unshaded samples during the first 5 days after deposition. UV-B was the most important parameter explaining the decay rate of E. coli populations. A comparison of the decay behaviors among all markers indicated that enterococcus concentrations exhibit a better correlation with the MST markers than E. coli concentrations. Our results indicate that bovine-associated MST markers can survive in cow pats for at least 1 month after excretion, and although their decay dynamic differs from the decay dynamic of E. coli populations, they seem to be reliable markers to use in combination with enterococci to monitor fecal pollution from pasture lands. PMID:24141130

  10. Strategic framework for education and training in Quality by Design (QbD) and process analytical technology (PAT).

    PubMed

    de Matas, Marcel; De Beer, Thomas; Folestad, Staffan; Ketolainen, Jarkko; Lindén, Hans; Lopes, João Almeida; Oostra, Wim; Weimer, Marco; Öhrngren, Per; Rantanen, Jukka

    2016-07-30

    The regulatory and technical landscape of the pharmaceutical field is rapidly evolving from one focused predominantly on development of small molecules, using well established manufacturing technologies towards an environment in which biologicals and complex modalities are being developed using advanced science and technology coupled with the application of modern Quality by Design (QbD) principles. In order that Europe keeps pace with these changes and sustains its position as major player in the development and commercialization of medicines, it is essential that measures are put in place to maintain a highly skilled workforce. A number of challenges however exist to equipping academic, industrial and health agency staff with the requisite knowledge, skills and experience to develop the next generation of medicines. In this regard, the EUFEPS QbD and PAT Sciences Network has proposed a structured framework for education, training and continued professional development, which comprises a number of pillars covering the fundamental principles of modern pharmaceutical development including the underpinning aspects of science, engineering and technology innovation. The framework is not prescriptive and is not aimed at describing specific course content in detail. It should however be used as a point of reference for those institutions delivering pharmaceutical based educational courses, to ensure that the necessary skills, knowledge and experience for successful pharmaceutical development are maintained. A positive start has been made and a number of examples of formal higher education courses and short training programs containing elements of this framework have been described. The ultimate vision for this framework however, is to see widespread adoption and proliferation of this curriculum with it forming the backbone of QbD and PAT science based skills development.

  11. Decay of fecal indicator bacterial populations and bovine-associated source-tracking markers in freshly deposited cow pats.

    PubMed

    Oladeinde, Adelumola; Bohrmann, Thomas; Wong, Kelvin; Purucker, S T; Bradshaw, Ken; Brown, Reid; Snyder, Blake; Molina, Marirosa

    2014-01-01

    Understanding the survival of fecal indicator bacteria (FIB) and microbial source-tracking (MST) markers is critical to developing pathogen fate and transport models. Although pathogen survival in water microcosms and manure-amended soils is well documented, little is known about their survival in intact cow pats deposited on pastures. We conducted a study to determine decay rates of fecal indicator bacteria (Escherichia coli and enterococci) and bovine-associated MST markers (CowM3, Rum-2-bac, and GenBac) in 18 freshly deposited cattle feces from three farms in northern Georgia. Samples were randomly assigned to shaded or unshaded treatment in order to determine the effects of sunlight, moisture, and temperature on decay rates. A general linear model (GLM) framework was used to determine decay rates. Shading significantly decreased the decay rate of the E. coli population (P < 0.0001), with a rate of -0.176 day(-1) for the shaded treatment and -0.297 day(-1) for the unshaded treatment. Shading had no significant effect on decay rates of enterococci, CowM3, Rum-2-bac, and GenBac (P > 0.05). In addition, E. coli populations showed a significant growth rate (0.881 day(-1)) in the unshaded samples during the first 5 days after deposition. UV-B was the most important parameter explaining the decay rate of E. coli populations. A comparison of the decay behaviors among all markers indicated that enterococcus concentrations exhibit a better correlation with the MST markers than E. coli concentrations. Our results indicate that bovine-associated MST markers can survive in cow pats for at least 1 month after excretion, and although their decay dynamic differs from the decay dynamic of E. coli populations, they seem to be reliable markers to use in combination with enterococci to monitor fecal pollution from pasture lands.

  12. The Rising Cost of Higher Education: Charles Eliot's "Free Money" Strategy and the Beginning of Howard Bowen's "Revenue Theory of Cost," 1869-1979

    ERIC Educational Resources Information Center

    Kimball, Bruce A.

    2014-01-01

    In order to explain the rising cost of higher education, economist Howard Bowen in 1980 proposed his "famous law" of institutional finance. Bowen based his "revenue theory of cost" on a study of aggregate quantitative data extending from 1929 to 1979. Neither he nor subsequent economists asked whether or how that…

  13. A Tale of Two Melt Rocks: Equilibration and Metal/Sulfide-Silicate Segregation in the L7 Chondrites PAT 91501 and LEW 88663

    NASA Astrophysics Data System (ADS)

    Harvey, R. P.

    1993-07-01

    Type 7 ordinary chondrites have experienced temperatures near or beyond those necessary for partial melting. Two recently collected Antarctic specimens, PAT91501 (PAT) and LEW88663 (LEW), have been tentatively identified as L7 chondrites based on mineral and oxygen isotope compositions [1,2]. The petrology and mineralogy of these meteorites suggests that they have undergone significant metal/sulfide-silicate segregation, with implications for meteorite parent bodies. PAT consists of an equigranular contact-framework of nearly euhedral olivine grains, with interstitial spaces filled by plagioclase, pyroxenes, and several minor phases. Ortho- and clinopyroxene occur in an exsolution relationship. Olivine and pyroxene are highly equilibrated, varying <<1% in Fe-endmember content. Pyroxene equilibration temperatures calculated for PAT using the methods of [3] are self-consistent at about 1180 degrees C. In thin section, PAT contains only traces of metal, as tiny isolated blebs in sulfide grains; large (>1 cm) globular sulfide inclusions are seen in hand-sample [1], but are not present in the section examined. LEW was originally classified as an achondrite with olivine and pyroxene compositions similar to those in L chondrites [2]. Metal is absent in LEW, although the specimen is small and heavily rusted, making it impossible to gauge the original metal content. Olivine grains are commonly rounded in shape and seldom in contact with more than a few other grains. LEW olivine and pyroxene are also highly equilibrated. Veins of Ni-bearing metal oxides and sulfides are common. Both low- and high-Ca pyroxene occur as discrete grains, orthopyroxene often poikilitically enclosing olivine. Pyroxene equilibration temperatures for LEW are more variable than those for PAT and consistently lower, with an average around 900 degrees C. The various textural and compositional characteristics of PAT and LEW suggest they have experienced partial melting to varying degrees. Both visually

  14. Activation of hormone-sensitive lipase requires two steps, protein phosphorylation and binding to the PAT-1 domain of lipid droplet coat proteins.

    PubMed

    Wang, Hong; Hu, Liping; Dalen, Knut; Dorward, Heidi; Marcinkiewicz, Amy; Russell, Deanna; Gong, Dawei; Londos, Constantine; Yamaguchi, Tomohiro; Holm, Cecilia; Rizzo, Mark A; Brasaemle, Dawn; Sztalryd, Carole

    2009-11-13

    Lipolysis is an important metabolic pathway controlling energy homeostasis through degradation of triglycerides stored in lipid droplets and release of fatty acids. Lipid droplets of mammalian cells are coated with one or more members of the PAT protein family, which serve important functions in regulating lipolysis. In this study, we investigate the mechanisms by which PAT family members, perilipin A, adipose differentiation-related protein (ADFP), and LSDP5, control lipolysis catalyzed by hormone-sensitive lipase (HSL), a major lipase in adipocytes and several non-adipose cells. We applied fluorescence microscopic tools to analyze proteins in situ in cultured Chinese hamster ovary cells using fluorescence recovery after photobleaching and anisotropy Forster resonance energy transfer. Fluorescence recovery after photobleaching data show that ADFP and LSDP5 exchange between lipid droplet and cytoplasmic pools, whereas perilipin A does not. Differences in protein mobility do not correlate with PAT protein-mediated control of lipolysis catalyzed by HSL or endogenous lipases. Forster resonance energy transfer and co-immunoprecipitation experiments reveal that each of the three PAT proteins bind HSL through interaction of the lipase with amino acids within the highly conserved amino-terminal PAT-1 domain. ADFP and LSDP5 bind HSL under basal conditions, whereas phosphorylation of serine residues within three amino-terminal protein kinase A consensus sequences of perilipin A is required for HSL binding and maximal lipolysis. Finally, protein kinase A-mediated phosphorylation of HSL increases lipolysis in cells expressing ADFP or LSDP5; in contrast, phosphorylation of perilipin A exerts the major control over HSL-mediated lipolysis when perilipin is the main lipid droplet protein. PMID:19717842

  15. Multi-parameter flow cytometry as a process analytical technology (PAT) approach for the assessment of bacterial ghost production.

    PubMed

    Langemann, Timo; Mayr, Ulrike Beate; Meitz, Andrea; Lubitz, Werner; Herwig, Christoph

    2016-01-01

    Flow cytometry (FCM) is a tool for the analysis of single-cell properties in a cell suspension. In this contribution, we present an improved FCM method for the assessment of E-lysis in Enterobacteriaceae. The result of the E-lysis process is empty bacterial envelopes-called bacterial ghosts (BGs)-that constitute potential products in the pharmaceutical field. BGs have reduced light scattering properties when compared with intact cells. In combination with viability information obtained from staining samples with the membrane potential-sensitive fluorescent dye bis-(1,3-dibutylarbituric acid) trimethine oxonol (DiBAC4(3)), the presented method allows to differentiate between populations of viable cells, dead cells, and BGs. Using a second fluorescent dye RH414 as a membrane marker, non-cellular background was excluded from the data which greatly improved the quality of the results. Using true volumetric absolute counting, the FCM data correlated well with cell count data obtained from colony-forming units (CFU) for viable populations. Applicability of the method to several Enterobacteriaceae (different Escherichia coli strains, Salmonella typhimurium, Shigella flexneri 2a) could be shown. The method was validated as a resilient process analytical technology (PAT) tool for the assessment of E-lysis and for particle counting during 20-l batch processes for the production of Escherichia coli Nissle 1917 BGs. PMID:26521248

  16. Multivariate analysis in the pharmaceutical industry: enabling process understanding and improvement in the PAT and QbD era.

    PubMed

    Ferreira, Ana P; Tobyn, Mike

    2015-01-01

    In the pharmaceutical industry, chemometrics is rapidly establishing itself as a tool that can be used at every step of product development and beyond: from early development to commercialization. This set of multivariate analysis methods allows the extraction of information contained in large, complex data sets thus contributing to increase product and process understanding which is at the core of the Food and Drug Administration's Process Analytical Tools (PAT) Guidance for Industry and the International Conference on Harmonisation's Pharmaceutical Development guideline (Q8). This review is aimed at providing pharmaceutical industry professionals an introduction to multivariate analysis and how it is being adopted and implemented by companies in the transition from "quality-by-testing" to "quality-by-design". It starts with an introduction to multivariate analysis and the two methods most commonly used: principal component analysis and partial least squares regression, their advantages, common pitfalls and requirements for their effective use. That is followed with an overview of the diverse areas of application of multivariate analysis in the pharmaceutical industry: from the development of real-time analytical methods to definition of the design space and control strategy, from formulation optimization during development to the application of quality-by-design principles to improve manufacture of existing commercial products.

  17. 3D noninvasive, high-resolution imaging using a photoacoustic tomography (PAT) system and rapid wavelength-cycling lasers

    NASA Astrophysics Data System (ADS)

    Sampathkumar, Ashwin; Gross, Daniel; Klosner, Marc; Chan, Gary; Wu, Chunbai; Heller, Donald F.

    2015-05-01

    Globally, cancer is a major health issue as advances in modern medicine continue to extend the human life span. Breast cancer ranks second as a cause of cancer death in women in the United States. Photoacoustic (PA) imaging (PAI) provides high molecular contrast at greater depths in tissue without the use of ionizing radiation. In this work, we describe the development of a PA tomography (PAT) system and a rapid wavelength-cycling Alexandrite laser designed for clinical PAI applications. The laser produces 450 mJ/pulse at 25 Hz to illuminate the entire breast, which eliminates the need to scan the laser source. Wavelength cycling provides a pulse sequence in which the output wavelength repeatedly alternates between 755 nm and 797 nm rapidly within milliseconds. We present imaging results of breast phantoms with inclusions of different sizes at varying depths, obtained with this laser source, a 5-MHz 128-element transducer and a 128-channel Verasonics system. Results include PA images and 3D reconstruction of the breast phantom at 755 and 797 nm, delineating the inclusions that mimic tumors in the breast.

  18. A PAT approach for the on-line monitoring of pharmaceutical co-crystals formation with near infrared spectroscopy.

    PubMed

    Sarraguça, Mafalda C; Ribeiro, Paulo R S; Santos, Adenilson O; Silva, Marta C D; Lopes, João A

    2014-08-25

    Cocrystals represent a class of crystalline solids consisting of two or more molecular species usually held together by non-covalent bonds. Pharmaceutical cocrystals can alter the physicochemical properties of the active pharmaceutical ingredient to improve solubility, dissolution rate, particle properties and stability. This work presents a process analytical technology (PAT) approach to monitor on-line the cocrystallization of furosemide and adenine by solvent evaporation using near infrared spectroscopy (NIRS). Furosemide and adenine were added to a small volume of methanol in a beaker and stirred on an orbital stirring table during 8h at room temperature. The on-line monitoring was performed with a FT-NIR spectrometer fitted with a reflectance fiber optic probe. Monitoring was performed with the probe tip placed 1cm above the cocrystallization medium to avoid interference with the cocrystallization process. Cocrystals were vacuum dried to remove residual solvent and characterized off-line by NIRS, MIRS, DSC and XRPD. Results demonstrate that it was possible to follow the main cocrystallization events on-line. PMID:24907598

  19. Multi-parameter flow cytometry as a process analytical technology (PAT) approach for the assessment of bacterial ghost production.

    PubMed

    Langemann, Timo; Mayr, Ulrike Beate; Meitz, Andrea; Lubitz, Werner; Herwig, Christoph

    2016-01-01

    Flow cytometry (FCM) is a tool for the analysis of single-cell properties in a cell suspension. In this contribution, we present an improved FCM method for the assessment of E-lysis in Enterobacteriaceae. The result of the E-lysis process is empty bacterial envelopes-called bacterial ghosts (BGs)-that constitute potential products in the pharmaceutical field. BGs have reduced light scattering properties when compared with intact cells. In combination with viability information obtained from staining samples with the membrane potential-sensitive fluorescent dye bis-(1,3-dibutylarbituric acid) trimethine oxonol (DiBAC4(3)), the presented method allows to differentiate between populations of viable cells, dead cells, and BGs. Using a second fluorescent dye RH414 as a membrane marker, non-cellular background was excluded from the data which greatly improved the quality of the results. Using true volumetric absolute counting, the FCM data correlated well with cell count data obtained from colony-forming units (CFU) for viable populations. Applicability of the method to several Enterobacteriaceae (different Escherichia coli strains, Salmonella typhimurium, Shigella flexneri 2a) could be shown. The method was validated as a resilient process analytical technology (PAT) tool for the assessment of E-lysis and for particle counting during 20-l batch processes for the production of Escherichia coli Nissle 1917 BGs.

  20. Advanced retorting, microwave assisted thermal sterilization (MATS), and pressure assisted thermal sterilization (PATS) to process meat products.

    PubMed

    Barbosa-Cánovas, Gustavo V; Medina-Meza, Ilce; Candoğan, Kezban; Bermúdez-Aguirre, Daniela

    2014-11-01

    Conventional thermal processes have been very reliable in offering safe sterilized meat products, but some of those products are of questionable overall quality. Flavor, aroma, and texture, among other attributes, are significantly affected during such processes. To improve those quality attributes, alternative approaches to sterilizing meat and meat products have been explored in the last few years. Most of the new strategies for sterilizing meat products rely on using thermal approaches, but in a more efficient way than in conventional methods. Some of these emerging technologies have proven to be reliable and have been formally approved by regulatory agencies such as the FDA. Additional work needs to be done in order for these technologies to be fully adopted by the food industry and to optimize their use. Some of these emerging technologies for sterilizing meat include pressure assisted thermal sterilization (PATS), microwaves, and advanced retorting. This review deals with fundamental and applied aspects of these new and very promising approaches to sterilization of meat products.

  1. Development of a multivariate light-induced fluorescence (LIF) PAT tool for in-line quantitative analysis of pharmaceutical granules in a V-blender.

    PubMed

    Guay, Jean-Maxime; Lapointe-Garant, Pierre-Philippe; Gosselin, Ryan; Simard, Jean-Sébastien; Abatzoglou, Nicolas

    2014-04-01

    Process analytical technologies (PAT) enable process insight, process control and real-time testing. Light-induced fluorescence (LIF) spectroscopy is especially well suited for low-concentration ingredients as, in many cases, it is the most sensitive probe of the in-line PAT toolbox. This study is aimed at verifying the applicability of a multivariate LIF analyzer to monitor granulated powder blends in industrial settings. Its targets are to: (1) evaluate the critical parameters of powders to obtain robust, precise and accurate concentration predictions and (2) assess technology performance for in-line monitoring of blending operations. Varying dye properties, moisture levels and particle sizes have been shown to have the most significant impact on fluorescence emission. Reliable quantitative models can be obtained by controlling and/or mitigating these factors. PMID:24373731

  2. Effects of the Activity of Coprophagous Insects on Greenhouse Gas Emissions from Cattle Dung Pats and Changes in Amounts of Nitrogen, Carbon, and Energy.

    PubMed

    Iwasa, Mitsuhiro; Moki, Yukari; Takahashi, Junichi

    2015-02-01

    Effects of coprophagous insects on greenhouse gas emissions from cattle dung pats were investigated during the initial stage in the decomposition of dung, with accompanying changes in nitrogen, carbon, and energy content. We set up three treatments with adults of Caccobius jessoensis Harold (dung beetle) and larvae of the fly Neomyia cornicina (F.): 1) dung with dung beetles; 2) dung with fly larvae; and 3) dung without insects. In these treatments, the gas flux was measured from air flow exiting the glass containers connected with an in vitro continuous gas analysis system. Total gas fluxes from dung pats with fly larvae were lowest in carbon dioxide (CO2), methane (CH4), and nitrous oxide (N2O). The presence of dung beetles significantly increased CO2 flux from dung, but reduced CH4 flux compared with dung without insects. Fluxes of N2O from dung pats with dung beetles and without insects had distinct peaks at different times after the start of the experiment, while N2O from dung with fly larvae was emitted in extremely low levels throughout the experiment. Carbon (C) content in dung with beetles was significantly lower than that of untreated dung pats designated as fresh dung, whereas that of dung with fly larvae was higher than dung with beetles and without insects. Nitrogen (N) content was significantly lower in dung with fly larvae than the other treatments. Contents of C and N in fly pupae were 35.87 and 8.05%, respectively. During the larval growth of the fly, energy accumulated in the fly body was 2,830 J/g.

  3. Effects of the Activity of Coprophagous Insects on Greenhouse Gas Emissions from Cattle Dung Pats and Changes in Amounts of Nitrogen, Carbon, and Energy.

    PubMed

    Iwasa, Mitsuhiro; Moki, Yukari; Takahashi, Junichi

    2015-02-01

    Effects of coprophagous insects on greenhouse gas emissions from cattle dung pats were investigated during the initial stage in the decomposition of dung, with accompanying changes in nitrogen, carbon, and energy content. We set up three treatments with adults of Caccobius jessoensis Harold (dung beetle) and larvae of the fly Neomyia cornicina (F.): 1) dung with dung beetles; 2) dung with fly larvae; and 3) dung without insects. In these treatments, the gas flux was measured from air flow exiting the glass containers connected with an in vitro continuous gas analysis system. Total gas fluxes from dung pats with fly larvae were lowest in carbon dioxide (CO2), methane (CH4), and nitrous oxide (N2O). The presence of dung beetles significantly increased CO2 flux from dung, but reduced CH4 flux compared with dung without insects. Fluxes of N2O from dung pats with dung beetles and without insects had distinct peaks at different times after the start of the experiment, while N2O from dung with fly larvae was emitted in extremely low levels throughout the experiment. Carbon (C) content in dung with beetles was significantly lower than that of untreated dung pats designated as fresh dung, whereas that of dung with fly larvae was higher than dung with beetles and without insects. Nitrogen (N) content was significantly lower in dung with fly larvae than the other treatments. Contents of C and N in fly pupae were 35.87 and 8.05%, respectively. During the larval growth of the fly, energy accumulated in the fly body was 2,830 J/g. PMID:26308812

  4. [The Patient Rights Act (PatRG)--part 1: legislative procedure, treatment contract, contracting parties and their obligations to cooperate and inform].

    PubMed

    Parzeller, Markus; Zedler, Barbara

    2013-01-01

    The article deals with the new regulations in the German Civil Code (BGB) which came into effect in Germany on 26 Feb 2013 as the Patient Rights Act (PatRG). In Part I, the legislative procedure, the treatment contract and the contracting parties (Section 630a Civil Code), the applicable regulations (Section 630b Civil Code) and the obligations to cooperate and inform (Section 630c Civil Code) are discussed and critically analysed.

  5. ePAT: a simple method to tag adenylated RNA to measure poly(A)-tail length and other 3' RACE applications.

    PubMed

    Jänicke, Amrei; Vancuylenberg, John; Boag, Peter R; Traven, Ana; Beilharz, Traude H

    2012-06-01

    The addition of a poly(A)-tail to the 3' termini of RNA molecules influences stability, nuclear export, and efficiency of translation. In the cytoplasm, dynamic changes in the length of the poly(A)-tail have long been recognized as reflective of the switch between translational silence and activation. Thus, measurement of the poly(A)-tail associated with any given mRNA at steady-state can serve as a surrogate readout of its translation-state. Here, we describe a simple new method to 3'-tag adenylated RNA in total RNA samples using the intrinsic property of Escherichia coli DNA polymerase I to extend an RNA primer using a DNA template. This tag can serve as an anchor for cDNA synthesis and subsequent gene-specific PCR to assess poly(A)-tail length. We call this method extension Poly(A) Test (ePAT). The ePAT approach is as efficient as traditional Ligation-Mediated Poly(A) Test (LM-PAT) assays, avoids problems of internal priming associated with oligo-dT-based methods, and allows for the accurate analysis of both the poly(A)-tail length and alternate 3' UTR usage in 3' RACE applications.

  6. Psychology, not educational neuroscience, is the way forward for improving educational outcomes for all children: Reply to Gabrieli (2016) and Howard-Jones et al. (2016).

    PubMed

    Bowers, Jeffrey S

    2016-10-01

    In Bowers (2016), I argued that there are (a) practical problems with educational neuroscience (EN) that explain why there are no examples of EN improving teaching and (b) principled problems with the logic motivating EN that explain why it is likely that there never will be. In the following article, I consider the main responses raised by both Gabrieli (2016) and Howard-Jones et al. (2016) and find them all unconvincing. Following this exchange, there are still no examples of EN providing new insights to teaching in the classroom, there are still no examples of EN providing new insights to remedial instructions for individuals, and, as I detail in this article, there is no evidence that EN is useful for the diagnosis of learning difficulties. The authors have also failed to address the reasons why EN is unlikely to benefit educational outcomes in the future. Psychology, by contrast, can (and has) made important discoveries that can (and should) be used to improve teaching and diagnostic tests for learning difficulties. This is not a debate about whether science is relevant to education, rather it is about what sort of science is relevant. (PsycINFO Database Record PMID:27657442

  7. Psychology, not educational neuroscience, is the way forward for improving educational outcomes for all children: Reply to Gabrieli (2016) and Howard-Jones et al. (2016).

    PubMed

    Bowers, Jeffrey S

    2016-10-01

    In Bowers (2016), I argued that there are (a) practical problems with educational neuroscience (EN) that explain why there are no examples of EN improving teaching and (b) principled problems with the logic motivating EN that explain why it is likely that there never will be. In the following article, I consider the main responses raised by both Gabrieli (2016) and Howard-Jones et al. (2016) and find them all unconvincing. Following this exchange, there are still no examples of EN providing new insights to teaching in the classroom, there are still no examples of EN providing new insights to remedial instructions for individuals, and, as I detail in this article, there is no evidence that EN is useful for the diagnosis of learning difficulties. The authors have also failed to address the reasons why EN is unlikely to benefit educational outcomes in the future. Psychology, by contrast, can (and has) made important discoveries that can (and should) be used to improve teaching and diagnostic tests for learning difficulties. This is not a debate about whether science is relevant to education, rather it is about what sort of science is relevant. (PsycINFO Database Record

  8. 'DIRTY WORK', BUT SOMEONE HAS TO DO IT: HOWARD P. ROBERTSON AND THE REFEREEING PRACTICES OF PHYSICAL REVIEW IN THE 1930S.

    PubMed

    Lalli, Roberto

    2016-06-20

    In the 1930s the mathematical physicist Howard P. Robertson was the main referee of the journal Physical Review for papers concerning general relativity and related subjects. The rich correspondence between Robertson and the editors of the journal enables a historical investigation of the refereeing process of Physical Review at the time that it was becoming one of the most influential physics periodicals in the world. By focusing on this case study, the paper investigates two complementary aspects of the evolution of the refereeing process: first, the historical evolution of the refereeing practices in connection with broader contextual changes, and second, the attempts to define the activity of the referee, including the epistemic virtues required and the journal's functions according to the participants' categories. By exploring the tension between Robertson's idealized picture about how the referee should behave and the desire to promote his intellectual agenda, I show that the evaluation criteria that Robertson employed were contextually dependent and I argue that, in the 1930s, through his reports the referee had an enormous power in defining what direction future research should take. PMID:27386715

  9. 'DIRTY WORK', BUT SOMEONE HAS TO DO IT: HOWARD P. ROBERTSON AND THE REFEREEING PRACTICES OF PHYSICAL REVIEW IN THE 1930S.

    PubMed

    Lalli, Roberto

    2016-06-20

    In the 1930s the mathematical physicist Howard P. Robertson was the main referee of the journal Physical Review for papers concerning general relativity and related subjects. The rich correspondence between Robertson and the editors of the journal enables a historical investigation of the refereeing process of Physical Review at the time that it was becoming one of the most influential physics periodicals in the world. By focusing on this case study, the paper investigates two complementary aspects of the evolution of the refereeing process: first, the historical evolution of the refereeing practices in connection with broader contextual changes, and second, the attempts to define the activity of the referee, including the epistemic virtues required and the journal's functions according to the participants' categories. By exploring the tension between Robertson's idealized picture about how the referee should behave and the desire to promote his intellectual agenda, I show that the evaluation criteria that Robertson employed were contextually dependent and I argue that, in the 1930s, through his reports the referee had an enormous power in defining what direction future research should take.

  10. Revolutionary changes in medicine and ophthalmology--the St. Louis, MO, effect: Homer G. Phillips Hospital and Dr. Howard Phillip venable.

    PubMed Central

    Johnson, Andrew S.

    2003-01-01

    PURPOSE: To provide information on the revolutionary changes in medicine, particularly on the development of African-Americans in ophthalmology, created by Homer G. Phillips Hospital in St. Louis and the late Dr. Howard Phillip Venable. METHODS: Very little has been written about Homer G. Phillips Hospital and Dr. H. Phillip Venable. Through personal interviews with physicians trained by Dr. Venable and literature review, I was able to obtain information on Homer G. Phillips Hospital and Dr. Venable's influence in breaking the color line in medicine and ophthalmology. RESULTS: Homer G. Phillips Hospital in St. Louis was a major teaching hospital for African-American doctors. Dr. Venable was one of a few African-American ophthalmologists in the 1940s. Dr. Venable then trained approximately 40 African-American ophthalmologists at Homer G. Phillips Hospital between 1943 to 1979, when the hospital closed. CONCLUSION: The ophthalmologists trained by Dr. Venable have gone forward to treat thousands of patients with blinding eye diseases such as glaucoma and diabetes, and to influence others to become ophthalmologists. Although the hospital has closed and Dr. Venable has passed away, their existence has reformed the medical treatment received by African-Americans and other people. Images p484-a PMID:12856914

  11. Mutation of sepJ reduces the intercellular signal range of a hetN-dependent paracrine signal, but not of a patS-dependent signal, in the filamentous cyanobacterium Anabaena sp. strain PCC 7120.

    PubMed

    Rivers, Orion S; Videau, Patrick; Callahan, Sean M

    2014-12-01

    Formation and maintenance of a periodic pattern of nitrogen-fixing cells called heterocysts by the filamentous cyanobacterium Anabaena sp. strain PCC 7120 is dependent on regulators encoded by patS and hetN. In this study, genetic mosaic filaments that consisted of cells engineered to produce one of the developmental regulators flanked by target cells capable of reporting the activity of the developmental regulator were used to investigate the intercellular movement of patS- and hetN-dependent activity. We provide evidence that hetN encodes a paracrine signal with a signal range of several cells. The signal that moved between cells did not include the C-terminus of the annotated HetN protein as indicated by similar signal ranges from source cells expressing either hetN-YFP or hetN alone, despite a lack of intercellular exchange of the HetN-YFP fusion protein. Deletion of sepJ, which has been shown to encode a component of intercellular channels, caused a significant decrease in the signal range of hetN expressed from source cells but not of patS. These results are consistent with symplastic transport of a paracrine hetN-dependent signal between vegetative cells of Anabaena.

  12. Introducing process analytical technology (PAT) in filamentous cultivation process development: comparison of advanced online sensors for biomass measurement.

    PubMed

    Rønnest, Nanna Petersen; Stocks, Stuart M; Eliasson Lantz, Anna; Gernaey, Krist V

    2011-10-01

    The recent process analytical technology (PAT) initiative has put an increased focus on online sensors to generate process-relevant information in real time. Specifically for fermentation, however, introduction of online sensors is often far from straightforward, and online measurement of biomass is one of the best examples. The purpose of this study was therefore to compare the performance of various online biomass sensors, and secondly to demonstrate their use in early development of a filamentous cultivation process. Eight Streptomyces coelicolor fed-batch cultivations were run as part of process development in which the pH, the feeding strategy, and the medium composition were varied. The cultivations were monitored in situ using multi-wavelength fluorescence (MWF) spectroscopy, scanning dielectric (DE) spectroscopy, and turbidity measurements. In addition, we logged all of the classical cultivation data, such as the carbon dioxide evolution rate (CER) and the concentration of dissolved oxygen. Prediction models for the biomass concentrations were estimated on the basis of the individual sensors and on combinations of the sensors. The results showed that the more advanced sensors based on MWF and scanning DE spectroscopy did not offer any advantages over the simpler sensors based on dual frequency DE spectroscopy, turbidity, and CER measurements for prediction of biomass concentration. By combining CER, DE spectroscopy, and turbidity measurements, the prediction error was reduced to 1.5 g/l, corresponding to 6% of the covered biomass range. Moreover, by using multiple sensors it was possible to check the quality of the individual predictions and switch between the sensors in real time.

  13. Re-Os systematics and age of pyrite associated with stratiform Zn-Pb mineralization in the Howards Pass district, Yukon and Northwest Territories, Canada

    NASA Astrophysics Data System (ADS)

    Kelley, Karen D.; Selby, David; Falck, Hendrik; Slack, John F.

    2016-05-01

    Stratiform Zn-Pb deposits hosted in unmetamorphosed carbonaceous and siliceous mudstones of the Ordovician to Silurian Duo Lake Formation define the Howards Pass district in Yukon Territory and Northwest Territories, western Canada. Collectively, the deposits are amongst the largest in the world, containing drill-indicated and inferred resources of 423 Mt at 4.84 % Zn and 1.59 % Pb. Sulphide textures include (a) fine-scale laminations of sphalerite, galena, and pyrite from <0.05 mm to 1 cm thick, interbedded with carbonaceous sedimentary rock; (b) layers of coarse sulphide that are structurally controlled by microfolds; and (c) veins that cut bedding and sulphide laminations. The finely interlaminated nature of sulphides with mudstone has been used as evidence for syngenetic mineralizing processes, whereas paleomagnetic data determined on coarse layered sulphides suggest a Middle Jurassic age of mineralization. Here, we present new rhenium-osmium (Re-Os) isotopic data for 12 pyrite separates obtained from 4 laminated sulphide-rich samples from the XY Central (XYC) and Don (DON) deposits and for 1 unmineralized organic-rich mudstone ˜20 m stratigraphically below the sulphide-bearing zone. Pyrite separates that lack mudstone inclusions ("pure") from the XYC deposit contain 2.2 to 4.0 ppb Re and 93.4 to 123.4 ppt Os; pure pyrite from the DON deposit is significantly more enriched in Re and Os (34-37 ppb Re; 636.8-694.9 ppt Os). The 187Re/188Os values of pure pyrite separates from the XYC and DON deposits range from 137.6 to 197 and 182.1 to 201.4, respectively. Regression of all pure pyrite Re-Os data from both deposits yields an isochron age of 442 ± 14 Ma (MSWD = 7.4) and an initial 187Os/188Os (Osi) value of 0.71 ± 0.07. The Re-Os age indicates that the early phase of pyrite precipitation (and by inference, sphalerite and galena) occurred during the early Silurian, consistent with biostratigraphic ages of the host rocks. The Osi value of ˜0.8 for earliest

  14. Geochemistry of host rocks in the Howards Pass district, Yukon-Northwest Territories, Canada: implications for sedimentary environments of Zn-Pb and phosphate mineralization

    NASA Astrophysics Data System (ADS)

    Slack, John F.; Falck, Hendrik; Kelley, Karen D.; Xue, Gabriel G.

    2016-10-01

    Detailed lithogeochemical data are reported here on early Paleozoic sedimentary rocks that host the large Howards Pass stratiform Zn-Pb deposits in Yukon-Northwest Territories. Redox-sensitive trace elements (Mo, Re, V, U) and Ce anomalies in members of the Duo Lake Formation record significant environmental changes. During the deposition of lower footwall units (Pyritic siliceous and Calcareous mudstone members), bottom waters were anoxic and sulphidic, respectively; these members formed in a marginal basin that may have become increasingly restricted with time. Relative to lower members, a major environmental change is proposed for deposition of the overlying Lower cherty mudstone member, which contains phosphorite beds up to ˜0.8 m thick in the upper part, near the base of the Zn-Pb deposits. The presence of these beds, together with models for modern phosphorite formation, suggests P input from an upwelling system and phosphorite deposition in an upper slope or outer shelf setting. The overlying Active mudstone member contains stratabound to stratiform Zn-Pb deposits within black mudstone and gray calcareous mudstone. Data for unmineralized black mudstone in this member indicate deposition under diverse redox conditions from suboxic to sulphidic. Especially distinctive in this member are uniformly low ratios of light to heavy rare earth elements that are unique within the Duo Lake Formation, attributed here to the dissolution of sedimentary apatite by downward-percolating acidic metalliferous brines. Strata that overlie the Active member (Upper siliceous mudstone member) consist mainly of black mudstone with thin (0.5-1.5 cm) laminae of fine-grained apatite, recording continued deposition on an upper slope or outer shelf under predominantly suboxic bottom waters. Results of this study suggest that exploration for similar stratiform sediment-hosted Zn-Pb deposits should include the outer parts of ancient continental margins, especially at and near stratigraphic

  15. Seasonal migration and environmental conditions of Pacific halibut Hippoglossus stenolepis, elucidated from pop-up archival transmitting (PAT) tags

    USGS Publications Warehouse

    Loher, T.; Seitz, A.

    2006-01-01

    Pop-up archival transmitting (PAT) tags were used to study the fall migration of halibut in the Gulf of Alaska (GOA). We tagged 6 Pacific halibut Hippoglossus stenolepis on summer feeding grounds in the eastern GOA and another 6 in the western GOA from June 13 to August 6, 2002. The tags were programed to be released from the fish on January 15, 2003, at the height of the winter spawning season: 10 tags successfully detached, transmitted archived environmental data (depth and temperature), and generated accurate latitude-longitude coordinates shortly after pop-up; 2 tags deployed off SE Alaska were lost. The tags revealed that 6 fish had moved a considerable distance (>200 km) between tagging and pop-up, and all of these had moved northward to some extent. The longest of the observed migrations was from the southern Alaska Peninsula to Yakutat Bay, a linear displacement of 1153 km; 4 fish showed little evidence of geographic displacement, exhibiting migrations that ranged only from 30 to 69 km. Although 2 fish had moved inshore by the end of the tagging period, all other fish had moved offshore regardless of their overall migration distance. The precise timing of offshore movements varied, beginning as early as August and as late as January. These observations generally corroborate conventional tagging, indicating migration of halibut toward winter spawning grounds in the northern GOA, and movement of fish to deep water in fall. However, no single stereotypic migration behavior was apparent, and a variety of vertical movement patterns and temperature profiles were observed. Halibut spent most time in waters of 5 to 7??C, but experienced temperatures ranging from 2.6 to 11.6??C. Depth observations ranged from 0 to 736 m, with summertime activity concentrated in depths from 0 to 400 m, and halibut that exhibited offshore movement were typically observed at 300 to 700 m by mid-winter. Vertical movement (short-period changes in depth) varied among fish and over time

  16. Constitutive expression of McCHIT1-PAT enhances resistance to rice blast and herbicide, but does not affect grain yield in transgenic glutinous rice.

    PubMed

    Zeng, Xiao-Fang; Li, Lei; Li, Jian-Rong; Zhao, De-Gang

    2016-01-01

    To produce new rice blast- and herbicide-resistant transgenic rice lines, the McCHIT1 gene encoding the class I chitinase from Momordica charantia and the herbicide resistance gene PAT were introduced into Lailong (Oryza sativa L. ssp. Japonica), a glutinous local rice variety from Guizhou Province, People's Republic of China. Transgenic lines were identified by ß-glucuronidase (GUS) histochemical staining, PCR, and Southern blot analyses. Agronomic traits, resistance to rice blast and herbicide, chitinase activities, and transcript levels of McCHIT1 were assessed in the T2 progeny of three transgenic lines (L1, L8, and L10). The results showed that the introduction of McCHIT1-PAT into Lailong significantly enhanced herbicide and blast resistance. After infection with the blast fungus Magnaporthe oryzae, all of the T2 progeny exhibited less severe lesion symptoms than those of wild type. The disease indices were 100% for wild type, 65.66% for T2 transgenic line L1, 59.69% for T2 transgenic line L8, and 79.80% for T2 transgenic line L10. Transgenic lines expressing McCHIT1-PAT did not show a significant difference from wild type in terms of malondialdehyde (MDA) content, polyphenol oxidase (PPO) activity, and superoxide dismutase (SOD) activity in the leaves. However, after inoculation with M. oryzae, transgenic plants showed significantly higher SOD and PPO activities and lower MDA contents in leaves, compared with those in wild-type leaves. The transgenic and the wild-type plants did not show significant differences in grain yield parameters including plant height, panicles per plant, seeds per panicle, and 1000-grain weight. Therefore, the transgenic plants showed increased herbicide and blast resistance, with no yield penalty.

  17. [The Patient Rights Act (PatRG)--part 2: informed consent, duty to inform, documentation of treatment, access to the patient file].

    PubMed

    Parzeller, Markus; Zedler, Barbara

    2013-01-01

    The article deals with the new regulations in the German Civil Code (BGB) which came into effect in Germany on 26 Feb 2013 as the Patient Rights Act (PatRG). In the second part, the consent of the patient (Section 630d Civil Code), the physician's duties to inform the patient (Section 630e Civil Code), the documentation of treatment (Section 630f Civil Code), and the right of access to the patient file (Section 630g Civil Code) are discussed and critically analysed. PMID:24547617

  18. Implementation of an advanced hybrid MPC-PID control system using PAT tools into a direct compaction continuous pharmaceutical tablet manufacturing pilot plant.

    PubMed

    Singh, Ravendra; Sahay, Abhishek; Karry, Krizia M; Muzzio, Fernando; Ierapetritou, Marianthi; Ramachandran, Rohit

    2014-10-01

    It is desirable for a pharmaceutical final dosage form to be manufactured through a quality by design (QbD)-based approach rather than a quality by testing (QbT) approach. An automatic feedback control system coupled with PAT tools that is part of the QbD paradigm shift, has the potential to ensure that the pre-defined end product quality attributes are met in a time and cost efficient manner. In this work, an advanced hybrid MPC-PID control architecture coupled with real time inline/online monitoring tools and principal components analysis (PCA) based additional supervisory control layer has been proposed for a continuous direct compaction tablet manufacturing process. The advantages of both MPC and PID have been utilized in a hybrid scheme. The control hardware and software integration and implementation of the control system has been demonstrated using feeders and blending unit operation of a continuous tablet manufacturing pilot plant and an NIR based PAT tool. The advanced hybrid MPC-PID control scheme leads to enhanced control loop performance of the critical quality attributes in comparison to a regulatory (e.g. PID) control scheme indicating its potential to improve pharmaceutical product quality. PMID:24974987

  19. Nuclear microprobe determination of platinum quantitative distribution in rat brain tumors after cisplatin or carboplatin injection for PAT treatment of glioma

    NASA Astrophysics Data System (ADS)

    Ortega, R.; Biston, M.-C.; Devès, G.; Bohic, S.; Carmona, A.

    2005-04-01

    Conventional radiotherapy of high-grade glioma is unsuccessful since less than 50% of patients survive at 6 months, therefore glioma treatment is still challenging. A new radiotherapy procedure has been recently proposed, the photoactivation therapy (PAT), associating synchrotron radiation with a chemotherapy agent, such as cisplatin. PAT aims at using the monochromaticity and the very high brilliance of the synchrotron radiation for selective excitation of a high-Z compound introduced in tumor cell DNA to maximize the photoelectric effect probability, thus increasing local toxicity. Synchrotron irradiation of cisplatin at the platinum absorption K-edge resulted in a dramatic increase in life span relative to median survival time in the F98 glioma model in Fisher rat. In the purpose to optimize the platinum concentration into the tumor, the platinum content of irradiated target needs to be quantified. These results will enable to correlate injected dose to cellular platinum content in the tumor at the time of irradiation, and to study the spatial diffusion and distribution of the platinum into the tumor and the surrounding healthy tissues from the point of injection. Male Fisher 344 rats were inoculated with 103 F98 glioma cells. Thirteen days after stereotactic inoculation, intracerebral injection at the tumor site of 40 μg of carboplatin and 3 or 5 μg of cisplatin was performed. Platinum quantitative distribution in tumors and adjacent brain tissues was determined using μ-PIXE and μ-RBS analysis.

  20. The world-class Howard's Pass SEDEX Zn-Pb district, Selwyn Basin, Yukon. Part II: the roles of thermochemical and bacterial sulfate reduction in metal fixation

    NASA Astrophysics Data System (ADS)

    Gadd, Michael G.; Layton-Matthews, Daniel; Peter, Jan M.; Paradis, Suzanne; Jonasson, Ian R.

    2016-07-01

    The Howard's Pass district of sedimentary exhalative (SEDEX) Zn-Pb deposits is located in Yukon Territory and comprises 14 Zn-Pb deposits that contain an estimated 400.7 Mt of sulfide mineralization grading 4.5 % Zn and 1.5 % Pb. Mineralization is hosted in carbonaceous and calcareous and, to a lesser extent, siliceous mudstones. Pyrite is a minor but ubiquitous mineral in the host rocks stratigraphically above, within, and below mineralization. Petrographic analyses reveal that pyrite has a complex and protracted growth history, preserving multiple generations of pyrite within single grains. Sulfur isotope analysis of paragenetically complex pyrite by secondary ion mass spectrometry (SIMS) reveals that sulfur isotope compositions vary with textural zonation. Within the Zn-Pb deposits, framboidal pyrite is the earliest pyrite generation recognized, and this exclusively has negative δ34S values (mean = -16.6 ± 4.1 ‰; n = 55), whereas paragenetically later pyrite and galena possess positive δ34S values (mean = 29.1 ± 7.5 and 22.4 ± 3.0 ‰, n = 13 and 13, respectively). Previous studies found that sphalerite and galena mineral separates have exclusively positive δ34S values (mean = 16.8 ± 3.3 and 12.7 ± 2.8 ‰, respectively; Goodfellow and Jonasson 1986). These distinct sulfur isotope values are interpreted to reflect varying contributions of bacterially reduced seawater sulfate (negative; framboidal pyrite) and thermochemically reduced seawater sulfate and/or hydrothermal sulfate (positive; galena, sphalerite, later forms of pyrite). Textural evidence indicates that framboidal pyrite predates galena and sphalerite deposition. Collectively, the in situ and bulk sulfur isotope data are much more complex than δ34S values permitted by prevailing genetic models that invoke only biogenically reduced sulfur and coeval deposition of galena, sphalerite, and framboidal pyrite within a euxinic water column, and we present several lines of evidence that argue

  1. Isolation of the patC gene encoding the cystathionine beta-lyase of Lactobacillus delbrueckii subsp. bulgaricus and molecular analysis of inter-strain variability in enzyme biosynthesis.

    PubMed

    Aubel, Dominique; Germond, Jacques Edouard; Gilbert, Christophe; Atlan, Danièle

    2002-07-01

    The patC gene encoding the cystathionine beta-lyase (CBL) of Lactobacillus delbrueckii subsp. bulgaricus NCDO 1489 was cloned and expressed in Escherichia coli. Overexpression of CBL complemented the methionine auxotrophy of an E. coli metC mutant, demonstrating in vivo that this enzyme functions as a CBL. However, PatC is distinguishable from the MetC CBLs by a low identity in amino acid sequence, a sensitivity to iodoacetic acid, greater thermostability and a lower substrate affinity. Homologues of patC were detected in the 13 Lb. delbrueckii strains studied, but only seven of them showed CBL activity. In constrast to CBL(+) strains, all CBL-deficient strains analysed were auxotrophic for methionine. This supports the hypothesis that CBLs from lactobacilli are probably involved in methionine biosynthesis. Moreover, the results of this study suggest that post-transcriptional mechanisms account for the differences in CBL activities observed between strains of Lb. delbrueckii.

  2. Green synthesis and antimicrobial activity of silver nanoparticles using wild medicinal mushroom Ganoderma applanatum (Pers.) Pat. from Similipal Biosphere Reserve, Odisha, India.

    PubMed

    Mohanta, Yugal Kishore; Singdevsachan, Sameer Kumar; Parida, Umesh Kumar; Panda, Sujogya Kumar; Mohanta, Tapan Kumar; Bae, Hanhong

    2016-08-01

    In the present study, green synthesis and cost effective approach of silver nanoparticles using wild medicinal mushroom Ganoderma applanatum (Pers.) Pat. from Similipal Biosphere Reserve, Odisha, India is reported. The biosynthesised AgNPs were characterised using UV-visible spectroscopy, particle analyser and scanning electron microscopy studies. It was found by dynamic light scattering analysis, that the average size and charges of the AgNPs were 133.0 ± 0.361 nm and -6.01 ± 5.30 mV, respectively. Moreover, the Fourier transform infrared study was also conducted to identify the biomolecules or functional groups responsible for the reduction of Ag and stabilisation of the AgNPs. The potential biomedical application with reference to antimicrobial activity of the synthesised AgNPs was investigated against some pathogenic microorganisms viz. Escherichia coli, Bacillus subtilis, Staphylococcus epidermidis, Vibrio cholerae, Staphylococcus aureus and Shigella flexneri.

  3. Green synthesis and antimicrobial activity of silver nanoparticles using wild medicinal mushroom Ganoderma applanatum (Pers.) Pat. from Similipal Biosphere Reserve, Odisha, India.

    PubMed

    Mohanta, Yugal Kishore; Singdevsachan, Sameer Kumar; Parida, Umesh Kumar; Panda, Sujogya Kumar; Mohanta, Tapan Kumar; Bae, Hanhong

    2016-08-01

    In the present study, green synthesis and cost effective approach of silver nanoparticles using wild medicinal mushroom Ganoderma applanatum (Pers.) Pat. from Similipal Biosphere Reserve, Odisha, India is reported. The biosynthesised AgNPs were characterised using UV-visible spectroscopy, particle analyser and scanning electron microscopy studies. It was found by dynamic light scattering analysis, that the average size and charges of the AgNPs were 133.0 ± 0.361 nm and -6.01 ± 5.30 mV, respectively. Moreover, the Fourier transform infrared study was also conducted to identify the biomolecules or functional groups responsible for the reduction of Ag and stabilisation of the AgNPs. The potential biomedical application with reference to antimicrobial activity of the synthesised AgNPs was investigated against some pathogenic microorganisms viz. Escherichia coli, Bacillus subtilis, Staphylococcus epidermidis, Vibrio cholerae, Staphylococcus aureus and Shigella flexneri. PMID:27463787

  4. An integrated process analytical technology (PAT) approach to monitoring the effect of supercooling on lyophilization product and process parameters of model monoclonal antibody formulations.

    PubMed

    Awotwe-Otoo, David; Agarabi, Cyrus; Khan, Mansoor A

    2014-07-01

    The aim of the present study was to apply an integrated process analytical technology (PAT) approach to control and monitor the effect of the degree of supercooling on critical process and product parameters of a lyophilization cycle. Two concentrations of a mAb formulation were used as models for lyophilization. ControLyo™ technology was applied to control the onset of ice nucleation, whereas tunable diode laser absorption spectroscopy (TDLAS) was utilized as a noninvasive tool for the inline monitoring of the water vapor concentration and vapor flow velocity in the spool during primary drying. The instantaneous measurements were then used to determine the effect of the degree of supercooling on critical process and product parameters. Controlled nucleation resulted in uniform nucleation at lower degrees of supercooling for both formulations, higher sublimation rates, lower mass transfer resistance, lower product temperatures at the sublimation interface, and shorter primary drying times compared with the conventional shelf-ramped freezing. Controlled nucleation also resulted in lyophilized cakes with more elegant and porous structure with no visible collapse or shrinkage, lower specific surface area, and shorter reconstitution times compared with the uncontrolled nucleation. Uncontrolled nucleation however resulted in lyophilized cakes with relatively lower residual moisture contents compared with controlled nucleation. TDLAS proved to be an efficient tool to determine the endpoint of primary drying. There was good agreement between data obtained from TDLAS-based measurements and SMART™ technology. ControLyo™ technology and TDLAS showed great potential as PAT tools to achieve enhanced process monitoring and control during lyophilization cycles. PMID:24840395

  5. Kagami–Ogata syndrome: a clinically recognizable upd(14)pat and related disorder affecting the chromosome 14q32.2 imprinted region

    PubMed Central

    Ogata, Tsutomu; Kagami, Masayo

    2016-01-01

    Human chromosome 14q32.2 carries paternally expressed genes including DLK1 and RTL1, and maternally expressed genes including MEG3 and RTL1as, along with the germline-derived DLK1-MEG3 intergenic differentially methylated region (IG-DMR) and the postfertilization-derived MEG3-DMR. Consistent with this, paternal uniparental disomy 14 (upd(14)pat), and epimutations (hypermethylations) and microdeletions affecting the IG-DMR and/or the MEG3-DMR of maternal origin, result in a unique phenotype associated with characteristic face, a small bell-shaped thorax with coat-hanger appearance of the ribs, abdominal wall defects, placentomegaly and polyhydramnios. Recently, the name ‘Kagami–Ogata syndrome' (KOS) has been approved for this clinically recognizable disorder. Here, we review the current knowledge about KOS. Important findings include the following: (1) the facial ‘gestalt' and the increased coat-hanger angle constitute pathognomonic features from infancy through childhood/puberty; (2) the unmethylated IG-DMR and MEG3-DMR of maternal origin function as the imprinting control centers in the placenta and body respectively, with a hierarchical interaction regulated by the IG-DMR for the methylation pattern of the MEG3-DMR in the body; (3) RTL1 expression level becomes ~2.5 times increased in the absence of functional RTL1as-encoded microRNAs that act as a trans-acting repressor for RTL1; (4) excessive RTL1 expression and absent MEG expression constitute the primary underlying factor for the phenotypic development; and (5) upd(14)pat accounts for approximately two-thirds of KOS patients, and epimutations and microdeletions are identified with a similar frequency. Furthermore, we refer to diagnostic and therapeutic implications. PMID:26377239

  6. An integrated process analytical technology (PAT) approach to monitoring the effect of supercooling on lyophilization product and process parameters of model monoclonal antibody formulations.

    PubMed

    Awotwe-Otoo, David; Agarabi, Cyrus; Khan, Mansoor A

    2014-07-01

    The aim of the present study was to apply an integrated process analytical technology (PAT) approach to control and monitor the effect of the degree of supercooling on critical process and product parameters of a lyophilization cycle. Two concentrations of a mAb formulation were used as models for lyophilization. ControLyo™ technology was applied to control the onset of ice nucleation, whereas tunable diode laser absorption spectroscopy (TDLAS) was utilized as a noninvasive tool for the inline monitoring of the water vapor concentration and vapor flow velocity in the spool during primary drying. The instantaneous measurements were then used to determine the effect of the degree of supercooling on critical process and product parameters. Controlled nucleation resulted in uniform nucleation at lower degrees of supercooling for both formulations, higher sublimation rates, lower mass transfer resistance, lower product temperatures at the sublimation interface, and shorter primary drying times compared with the conventional shelf-ramped freezing. Controlled nucleation also resulted in lyophilized cakes with more elegant and porous structure with no visible collapse or shrinkage, lower specific surface area, and shorter reconstitution times compared with the uncontrolled nucleation. Uncontrolled nucleation however resulted in lyophilized cakes with relatively lower residual moisture contents compared with controlled nucleation. TDLAS proved to be an efficient tool to determine the endpoint of primary drying. There was good agreement between data obtained from TDLAS-based measurements and SMART™ technology. ControLyo™ technology and TDLAS showed great potential as PAT tools to achieve enhanced process monitoring and control during lyophilization cycles.

  7. Kagami-Ogata syndrome: a clinically recognizable upd(14)pat and related disorder affecting the chromosome 14q32.2 imprinted region.

    PubMed

    Ogata, Tsutomu; Kagami, Masayo

    2016-02-01

    Human chromosome 14q32.2 carries paternally expressed genes including DLK1 and RTL1, and maternally expressed genes including MEG3 and RTL1as, along with the germline-derived DLK1-MEG3 intergenic differentially methylated region (IG-DMR) and the postfertilization-derived MEG3-DMR. Consistent with this, paternal uniparental disomy 14 (upd(14)pat), and epimutations (hypermethylations) and microdeletions affecting the IG-DMR and/or the MEG3-DMR of maternal origin, result in a unique phenotype associated with characteristic face, a small bell-shaped thorax with coat-hanger appearance of the ribs, abdominal wall defects, placentomegaly and polyhydramnios. Recently, the name 'Kagami-Ogata syndrome' (KOS) has been approved for this clinically recognizable disorder. Here, we review the current knowledge about KOS. Important findings include the following: (1) the facial 'gestalt' and the increased coat-hanger angle constitute pathognomonic features from infancy through childhood/puberty; (2) the unmethylated IG-DMR and MEG3-DMR of maternal origin function as the imprinting control centers in the placenta and body respectively, with a hierarchical interaction regulated by the IG-DMR for the methylation pattern of the MEG3-DMR in the body; (3) RTL1 expression level becomes ~2.5 times increased in the absence of functional RTL1as-encoded microRNAs that act as a trans-acting repressor for RTL1; (4) excessive RTL1 expression and absent MEG expression constitute the primary underlying factor for the phenotypic development; and (5) upd(14)pat accounts for approximately two-thirds of KOS patients, and epimutations and microdeletions are identified with a similar frequency. Furthermore, we refer to diagnostic and therapeutic implications.

  8. Determinants within the C-terminal domain of Streptomyces lividans acetyl-CoA synthetase that block acetylation of its active site lysine in vitro by the protein acetyltransferase (Pat) enzyme.

    PubMed

    Tucker, Alex C; Escalante-Semerena, Jorge C

    2014-01-01

    Reversible lysine acetylation (RLA) is a widespread regulatory mechanism that modulates the function of proteins involved in diverse cellular processes. A strong case has been made for RLA control exerted by homologues of the Salmonella enterica protein acetyltransferase (SePat) enzyme on the broadly distributed AMP-forming CoA ligase (a.k.a. acyl-CoA synthetases) family of metabolic enzymes, with acetyl-CoA synthetase (Acs) being the paradigm in the field. Here we investigate why the Acs homologue in Streptomyces lividans (SlAcs) is poorly acetylated in vitro by the S. lividans protein acetyltransferase (SlPat) enzyme. Chimeras of S. enterica Acs (SeAcs) and S. lividans Acs (SlAcs) constructed during the course of this work were acetylated by SlPatA in vitro, retained most of their activity, and were under RLA control in a heterologous host. We identified SeAcs residues N- and C-terminal to the target lysine that when introduced into SlAcs, rendered the latter under RLA control. These results lend further support to the idea that Pat enzymes interact with extensive surfaces of their substrates. Finally, we suggest that acetylation of SlAcs depends on factors or conditions other than those present in our in vitro system. We also discuss possible explanations why SlAcs is not controlled by RLA as defined in other bacterial species.

  9. CaMKII-MEDIATED PHOSPHORYLATION OF THE BOMBYX MORI LIPID STORAGE DROPLET PROTEIN-1 (BmLsd1), AN INSECT PAT FAMILY PROTEIN, IS ESSENTIAL FOR SILKMOTH SEX PHEROMONE BIOSYNTHESIS

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The structurally-related members of the PAT family of proteins, which are so name based on similarity amongst perilipin, adipophilin/adipocyte differentiation-related protein (ADRP), and tail-interacting protein of 47 kilodaltons (TIP47), are cytoplasmic lipid droplet (LD)-associated proteins charac...

  10. Hormone signaling linked to silkmoth sex pheromone biosynthesis involves Ca2+/calmodulin-dependent protein kinase II-mediated phosphorylation of the insect PAT family protein Bombyx mori lipid storage droplet protein-1(BmLsd)

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The structurally-related members of the PAT family of proteins, which are so name based on similarity amongst perilipin, adipophilin/adipocyte differentiation-related protein (ADRP), and tail-interacting protein of 47 kilodaltons (TIP47), are cytoplasmic lipid droplet (LD)-associated proteins charac...

  11. Implementation of an artificial neural network as a PAT tool for the prediction of temperature distribution within a pharmaceutical fluidized bed granulator.

    PubMed

    Korteby, Yasmine; Mahdi, Yassine; Azizou, Amel; Daoud, Kamel; Regdon, Géza

    2016-06-10

    In this study, a novel in-line measurement technique of the air temperature distribution during a granulation process using a conical fluidized bed was designed and built for the purpose of measuring the temperature under the Process Analytical Technology (PAT) and introduced to predict the establishment of temperature profiles. Three sets of thermocouples were used, placed at different positions covering the whole operating range, connected to data acquisition measurement hardware, allowing an in-line acquisition and recording of temperatures every second. The measurements throughout the fluidized bed were performed in a steady state by spraying a solution of PVP onto a lactose monohydrate powder bed in order to make predictions of the temperature distribution and the hydrodynamics of the bed during the granulation process using Artificial Neural Networks (ANNs) and to establish the different temperature profiles for different process conditions through the precise predicted information by the constructed, trained, validated and tested neural network. The model's testing results showed a strong prediction capacity of the effects of process variables. Indeed, the predicted temperature values obtained with the ANN model were in good agreement with the values measured with in-line reference method and hence the method can have an application as a predictive control tool. PMID:26993961

  12. Both Sm-domain and C-terminal extension of Lsm1 are important for the RNA-binding activity of the Lsm1-7-Pat1 complex.

    PubMed

    Chowdhury, Ashis; Raju, Kalidindi K; Kalurupalle, Swathi; Tharun, Sundaresan

    2012-05-01

    Lsm proteins are a ubiquitous family of proteins characterized by the Sm-domain. They exist as hexa- or heptameric RNA-binding complexes and carry out RNA-related functions. The Sm-domain is thought to be sufficient for the RNA-binding activity of these proteins. The highly conserved eukaryotic Lsm1 through Lsm7 proteins are part of the cytoplasmic Lsm1-7-Pat1 complex, which is an activator of decapping in the conserved 5'-3' mRNA decay pathway. This complex also protects mRNA 3'-ends from trimming in vivo. Purified Lsm1-7-Pat1 complex is able to bind RNA in vitro and exhibits a unique binding preference for oligoadenylated RNA (over polyadenylated and unadenylated RNA). Lsm1 is a key subunit that determines the RNA-binding properties of this complex. The normal RNA-binding activity of this complex is crucial for mRNA decay and 3'-end protection in vivo and requires the intact Sm-domain of Lsm1. Here, we show that though necessary, the Sm-domain of Lsm1 is not sufficient for the normal RNA-binding ability of the Lsm1-7-Pat1 complex. Deletion of the C-terminal domain (CTD) of Lsm1 (while keeping the Sm-domain intact) impairs mRNA decay in vivo and results in Lsm1-7-Pat1 complexes that are severely impaired in RNA binding in vitro. Interestingly, the mRNA decay and 3'-end protection defects of such CTD-truncated lsm1 mutants could be suppressed in trans by overexpression of the CTD polypeptide. Thus, unlike most Sm-like proteins, Lsm1 uniquely requires both its Sm-domain and CTD for its normal RNA-binding function.

  13. In-line and real-time process monitoring of a freeze drying process using Raman and NIR spectroscopy as complementary process analytical technology (PAT) tools.

    PubMed

    De Beer, T R M; Vercruysse, P; Burggraeve, A; Quinten, T; Ouyang, J; Zhang, X; Vervaet, C; Remon, J P; Baeyens, W R G

    2009-09-01

    The aim of the present study was to examine the complementary properties of Raman and near infrared (NIR) spectroscopy as PAT tools for the fast, noninvasive, nondestructive and in-line process monitoring of a freeze drying process. Therefore, Raman and NIR probes were built in the freeze dryer chamber, allowing simultaneous process monitoring. A 5% (w/v) mannitol solution was used as model for freeze drying. Raman and NIR spectra were continuously collected during freeze drying (one Raman and NIR spectrum/min) and the spectra were analyzed using principal component analysis (PCA) and multivariate curve resolution (MCR). Raman spectroscopy was able to supply information about (i) the mannitol solid state throughout the entire process, (ii) the endpoint of freezing (endpoint of mannitol crystallization), and (iii) several physical and chemical phenomena occurring during the process (onset of ice nucleation, onset of mannitol crystallization). NIR spectroscopy proved to be a more sensitive tool to monitor the critical aspects during drying: (i) endpoint of ice sublimation and (ii) monitoring the release of hydrate water during storage. Furthermore, via NIR spectroscopy some Raman observations were confirmed: start of ice nucleation, end of mannitol crystallization and solid state characteristics of the end product. When Raman and NIR monitoring were performed on the same vial, the Raman signal was saturated during the freezing step caused by reflected NIR light reaching the Raman detector. Therefore, NIR and Raman measurements were done on a different vial. Also the importance of the position of the probes (Raman probe above the vial and NIR probe at the bottom of the sidewall of the vial) in order to obtain all required critical information is outlined. Combining Raman and NIR spectroscopy for the simultaneous monitoring of freeze drying allows monitoring almost all critical freeze drying process aspects. Both techniques do not only complement each other, they also

  14. [José Juan Verocay, "el patólogo de Praga" (on occasion of the 100th anniversary of his habilitation in Prague)].

    PubMed

    Čech, P

    2012-04-01

    José Juan Verocay was born on June 16, 1876 in Paysandú, Uruguay to Italian immigrants; in 1887 they sent him to Cortina d'Ampezzo to learn languages first; he then graduated from the high school in Trento (1897) and from the German Medical Faculty in Prague (1904) where he, a disciple of Hanns Chiari, became the 1st demonstrator (1902), 3rd (1904) to 1st assistant (1905), and volunteer (from 1908) at the Department of Pathological Anatomy. He repeatedly substituted the professors Chiari (until 1906), Kretz (1907-1910), and Ghon (from 1910) during their absence. Anomalies and neoplasms prevailed among his research subjects. In the paper "Zur Kenntnis der »Neurofibrome«" (1910) he introduced the term "neurinoma" for a tumor with characteristic structures later named "Verocay bodies". On the basis of the paper he was habilitated for pathological anatomy as private docent at the German Medical Faculty in Prague (1911). During World War I he served for the Austro-Hungarian army at military hospitals in Chrudim (Bohemia) and Vienna. After the war he returned to Uruguay to work as a general practitioner in his native region (1919-1921), thereafter in Montevideo as head of pathological laboratories at the military hospital (1921-1925), at the Dental School (1925-1927), and at the Medical Faculty Department of Neurology (from March 19, 1927). As early as on May 3, 1927, however, he had to retire due to rapid worsening of his pulmonary tuberculosis. The renowned scientist remained a stranger in his own country ("el patólogo de Praga"); he never gained professorship except for a symbolic proclamation by devoted students on August 24, 1927, two days before he left for Europe to undergo treatment. On December 26, 1927 he died in Dubí (a spa near Teplice in Bohemia, Czechoslovakia, now Czech Republic).

  15. The world-class Howard's Pass SEDEX Zn-Pb district, Selwyn Basin, Yukon. Part I: trace element compositions of pyrite record input of hydrothermal, diagenetic, and metamorphic fluids to mineralization

    NASA Astrophysics Data System (ADS)

    Gadd, Michael G.; Layton-Matthews, Daniel; Peter, Jan M.; Paradis, Suzanne J.

    2016-03-01

    The Howard's Pass district, located in Yukon Territory, comprises 14 Zn-Pb sedimentary exhalative (SEDEX) deposits that collectively contain approximately 400.7 Mt grading at 4.5 % Zn and 1.5 % Pb. Sulfide mineralization is hosted in carbonaceous and calcareous to siliceous mudstones. Pyrite is a minor but ubiquitous component. Detailed petrographic analyses reveal that pyrite has a complex and protracted growth history, and multiple generations of pyrite are preserved in single grains. Combined electron probe microanalysis (EPMA) and laser ablation-inductively coupled mass plasma-mass spectrometry (LA-ICP-MS) of paragenetically complex pyrite reveal minor and trace element zonation that mimic textural features. These data provide information on the relative timing and cation content of depositional (i.e., ambient marine), hydrothermal, and metamorphic fluids. These data also identify a suite of nonore elements (Mn, As, Ag, Sb, and Tl) associated with the Zn-Pb mineralizing hydrothermal fluids. Lithogeochemical data and statistical results corroborate the microanalytical findings. These elements are associated with both syngenetic to earliest diagenetic pyrite and later diagenetic pyrite overgrowths, suggesting that SEDEX mineralization was not only the product of hydrothermal precipitates that settled on the seafloor, but also dense metalliferous brine also settled on, and percolated through, unconsolidated carbonaceous muds and precipitated metals. This genetic model is similar to that proposed for the Paleoproterozoic HYC Zn-Pb-Ag SEDEX deposit in northern Australia, and it is likely that common processes and ambient conditions led to the formation and preservation of both of these large SEDEX districts.

  16. A DNA electrochemical sensor prepared by electrodepositing zirconia on composite films of single-walled carbon nanotubes and poly(2,6-pyridinedicarboxylic acid), and its application to detection of the PAT gene fragment.

    PubMed

    Yang, Jie; Jiao, Kui; Yang, Tao

    2007-10-01

    Carboxyl group-functionalized single-walled carbon nanotubes (SWNTs) and 2,6-pyridinedicarboxylic acid (PDC) were electropolymerized by cyclic voltammetry on a glassy-carbon electrode (GCE) surface to form composite films (SWNTs/PDC). Zirconia was then electrodeposited on the SWNTs/PDC/GCE from an aqueous electrolyte containing ZrOCl2 and KCl by cycling the potential between -1.1 V and +0.7 V at a scan rate of 20 mV s(-1). DNA probes with a phosphate group at the 5' end were easily immobilized on the zirconia thin films, because of the strong affinity between zirconia and phosphate groups. The sensors were characterized by cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). EIS was used for label-free detection of the target DNA by measuring the increase of the electron transfer resistance (R(et)) of the electrode surface after the hybridization of the probe DNA with the target DNA. The PAT gene fragment and polymerase chain reaction (PCR) amplification of the NOS gene from transgenically modified beans were satisfactorily detected by use of this DNA electrochemical sensor. The dynamic range of detection of the sensor for the PAT gene fragment was from 1.0 x 10(-11) to 1.0 x 10(-6) mol L(-1) and the detection limit was 1.38 x 10(-12) mol L(-1). PMID:17851654

  17. Improving Adult Education for the 21st Century. Hearing Before the Subcommittee on 21st Century Competitiveness of the Committee on Education and the Workforce, House of Representatives, One Hundred Eighth Congress, First Session. Hearing held in Washington, DC, March 4, 2003.

    ERIC Educational Resources Information Center

    Congress of the U.S., Washington, DC. House Committee on Education and the Workforce.

    A hearing was held to discuss the prior four years of implementation of the Adult Education and Family Literacy Act, and to recommend further improvements. The opening statements of Chairman Howard McKeon and Dale E. Kildee introduce the meeting and discuss the importance of promoting an educated populace that will maintain the United States'…

  18. America's Teacher Colleges: Are They Making the Grade? Hearing before the Subcommittee on 21st Century Competitiveness of the Committee on Education and the Workforce. House of Representatives, One Hundred Eighth Congress, First Session (May 20, 2003).

    ERIC Educational Resources Information Center

    Congress of the U.S., Washington, DC. House Committee on Education and the Workforce.

    A hearing was held to explore whether U. S. teachers' colleges are doing an adequate job. Opening statements by Representative Howard P. McKeon and Representative Dale Kildee set the stage for the testimony of these witnesses: (1) Lisa Graham Keegan, Chief Executive Officer, Education Leaders Council; (2) Kati Haycock, Director, The Education…

  19. The Federal Role in K-12 Mathematics Reform. Hearing before the Subcommittee on Early Childhood, Youth and Families of the Committee on Education and the Workforce, House of Representatives, One Hundred Sixth Congress, Second Session (February 2, 2000).

    ERIC Educational Resources Information Center

    Congress of the U.S., Washington, DC. House Committee on Education and the Workforce.

    This hearing focuses on the federal role in K-12 mathematics reform. Prepared statements are provided from: Michael N. Castle, Chairman, Subcommittee on Early Childhood Youth, and Families; Dale E. Kildee, Ranking Member, Subcommittee on Early Childhood Youth, and Families; Howard P. "Buck" Mckeon, Chairman, Subcommittee on Postsecondary…

  20. Advancing Humanities Studies at Community, Technical, and Junior Colleges.

    ERIC Educational Resources Information Center

    Eisenberg, Diane U.; And Others

    The American Association of Community and Junior Colleges' (AACJC's) two-year Advancing the Humanities Project (AHP) has assisted selected community colleges in promoting the humanities on their campuses. Parts I and II of this report on the AHP present statements by Dale Parnell and Judith Jeffrey Howard about the AACJC's humanities initiatives…

  1. Simultaneous Enrichment of Cysteine-containing Peptides and Phosphopeptides Using a Cysteine-specific Phosphonate Adaptable Tag (CysPAT) in Combination with titanium dioxide (TiO2) Chromatography*

    PubMed Central

    Huang, Honggang; Haar Petersen, Martin; Ibañez-Vea, Maria; Lassen, Pernille S.; Larsen, Martin R.; Palmisano, Giuseppe

    2016-01-01

    Cysteine is a rare and conserved amino acid involved in most cellular functions. The thiol group of cysteine can be subjected to diverse oxidative modifications that regulate many physio-pathological states. In the present work, a Cysteine-specific Phosphonate Adaptable Tag (CysPAT) was synthesized to selectively label cysteine-containing peptides (Cys peptides) followed by their enrichment with titanium dioxide (TiO2) and subsequent mass spectrometric analysis. The CysPAT strategy was developed using a synthetic peptide, a standard protein and subsequently the strategy was applied to protein lysates from Hela cells, achieving high specificity and enrichment efficiency. In particular, for Cys proteome analysis, the method led to the identification of 7509 unique Cys peptides from 500 μg of HeLa cell lysate starting material. Furthermore, the method was developed to simultaneously enrich Cys peptides and phosphorylated peptides. This strategy was applied to SILAC labeled Hela cells subjected to 5 min epidermal growth factor (EGF) stimulation. In total, 10440 unique reversibly modified Cys peptides (3855 proteins) and 7339 unique phosphopeptides (2234 proteins) were simultaneously identified from 250 μg starting material. Significant regulation was observed in both phosphorylation and reversible Cys modification of proteins involved in EGFR signaling. Our data indicates that EGF stimulation can activate the well-known phosphorylation of EGFR and downstream signaling molecules, such as mitogen-activated protein kinases (MAPK1 and MAPK3), however, it also leads to substantial modulation of reversible cysteine modifications in numerous proteins. Several protein tyrosine phosphatases (PTPs) showed a reduction of the catalytic Cys site in the conserved putative phosphatase HC(X)5R motif indicating an activation and subsequent de-phosphorylation of proteins involved in the EGF signaling pathway. Overall, the CysPAT strategy is a straight forward, easy and promising

  2. Howard Parnes, MD | Division of Cancer Prevention

    Cancer.gov

    The Division of Cancer Prevention (DCP) conducts and supports research to determine a person's risk of cancer and to find ways to reduce the risk. This knowledge is critical to making progress against cancer because risk varies over the lifespan as genetic and epigenetic changes can transform healthy tissue into invasive cancer.

  3. Drama in the Dale: Transformation through Community Drama

    ERIC Educational Resources Information Center

    Ward, Julie; Mills, Helen Frances; Anderson, Alan

    2013-01-01

    During the winter of 2011-2012, Weardale, England, was the setting for an ambitious informal adult education project. In this rural area in the northeast part of the country, the local arts collective, Jack Drum Arts, established a community play project entitled "The Bonny Moorhen." This dramatic undertaking aimed to retell the story of…

  4. Dale Long Emergency Medical Service Providers Protection Act

    THOMAS, 112th Congress

    Sen. Leahy, Patrick J. [D-VT

    2011-02-17

    02/17/2011 Read twice and referred to the Committee on the Judiciary. (text of measure as introduced: CR S885) (All Actions) Tracker: This bill has the status IntroducedHere are the steps for Status of Legislation:

  5. Book Review: Caves and Karst of the Yorkshire Dales

    NASA Astrophysics Data System (ADS)

    Westaway, Rob

    2015-10-01

    The British Cave Research Association (BCRA) is the research division of the British Caving Association (BCA), itself the principal society in Britain for those interested in caving, with activities including provision of training and safety certification for cavers and managing access to caves. Although some UK cave-related research is carried out by academics, this tends to be restricted to archaeological investigations of caves that have served as human habitations, and to be focused more on the occupants than the caves themselves. In contrast, most cave exploration is undertaken as a leisure activity, under the auspices of clubs affiliated to the BCA/BCRA, this being indeed virtually the only field of Earth science where amateur investigators can continue to make significant discoveries. Many cave explorers are also affiliated with academic researchers, such as managers of dating laboratories; the synergy between these two groups is highly productive, having resulted for instance in the discovery and exploration in recent years of the vast Ogof Draenen cave system in South Wales, which probably dates back to the Early Pleistocene (e.g., Farrant et al., 2014).

  6. "The Day All of the Different Parts of Me Can Come Along": Intersectionality and U.S. Third World Feminism in the Poetry of Pat Parker and Willyce Kim.

    PubMed

    Van Ausdall, Mimi Iimuro

    2015-01-01

    This article brings to light the poetry of Pat Parker and Willyce Kim, two key figures within the 1970s and '80s women in print movement. While Parker and Kim have been rightly placed within African-American and Asian-American histories, respectively, and working-class and lesbian-feminist literary histories, their work is most fully understood within the context of U.S. Third World Feminism. Through close readings of poetic form and content in addition to engagement with current debates about intersectionality as a methodology, the article links Kim and Parker's works to central contributions of U.S. Third World Feminism such as intersectionality and power across and within difference that continue to influence feminist theory today. PMID:26075687

  7. "The Day All of the Different Parts of Me Can Come Along": Intersectionality and U.S. Third World Feminism in the Poetry of Pat Parker and Willyce Kim.

    PubMed

    Van Ausdall, Mimi Iimuro

    2015-01-01

    This article brings to light the poetry of Pat Parker and Willyce Kim, two key figures within the 1970s and '80s women in print movement. While Parker and Kim have been rightly placed within African-American and Asian-American histories, respectively, and working-class and lesbian-feminist literary histories, their work is most fully understood within the context of U.S. Third World Feminism. Through close readings of poetic form and content in addition to engagement with current debates about intersectionality as a methodology, the article links Kim and Parker's works to central contributions of U.S. Third World Feminism such as intersectionality and power across and within difference that continue to influence feminist theory today.

  8. Similar frequency of paternal uniparental disomy involving chromosome 20q (patUPD20q) in Japanese and Caucasian patients affected by sporadic pseudohypoparathyroidism type Ib (sporPHP1B).

    PubMed

    Takatani, Rieko; Minagawa, Masanori; Molinaro, Angelo; Reyes, Monica; Kinoshita, Kaori; Takatani, Tomozumi; Kazukawa, Itsuro; Nagatsuma, Misako; Kashimada, Kenichi; Sato, Kenichi; Matsushita, Kazuyuki; Nomura, Fumio; Shimojo, Naoki; Jüppner, Harald

    2015-10-01

    Pseudohypoparathyroidism type Ib (PHP1B) is caused by proximal tubular resistance to parathyroid hormone that occurs in most cases in the absence of Albright's Hereditary Osteodystrophy (AHO). Familial forms of PHP1B are caused by maternally inherited microdeletions within STX16, the gene encoding syntaxin 16, or within GNAS, a complex genetic locus on chromosome 20q13.3 encoding Gsα and several splice variants thereof. These deletions lead either to a loss-of-methylation affecting GNAS exon A/B alone or to epigenetic changes involving multiple differentially methylated regions (DMRs) within GNAS. Broad GNAS methylation abnormalities are also observed in most sporadic PHP1B (sporPHP1B) cases. However, with the exception of paternal uniparental disomy involving chromosome 20q (patUPD20q), the molecular mechanism leading to this disease variant remains unknown. We now investigated 23 Japanese sporPHP1B cases, who presented with hypocalcemia, hyperphosphatemia, elevated PTH levels, and occasionally with TSH elevations and mild AHO features. Age at diagnosis was 10.6 ± 1.45 years. Calcium, phosphate, and PTH were 6.3 ± 0.23 mg/dL, 7.7 ± 0.33 mg/dL, and 305 ± 34.5 pg/mL, respectively, i.e. laboratory findings that are indistinguishable from those previously observed for Caucasian sporPHP1B cases. All investigated patients showed broad GNAS methylation changes. Eleven individuals were homozygous for SNPs within exon NESP and a pentanucleotide repeat in exon A/B. Two of these patients furthermore revealed homozygosity for numerous microsatellite markers on chromosome 20q raising the possibility of patUPD20q, which was confirmed through the analysis of parental DNA. Based on this and our previous reports, paternal duplication of the chromosomal region comprising the GNAS locus appears to be a fairly common cause of sporPHP1B that is likely to occur with equal frequency in Caucasians and Asians. PMID:25997889

  9. Field Turf System Has Irrigation Down PAT

    ERIC Educational Resources Information Center

    Day, C. William

    1973-01-01

    Explains the process whereby Goshen High School (Indiana) acquired a football field that is never muddy, but which is never covered with expensive sheeting; and that has green grass the year around, but which no one ever sprinkles. It also offers firmness for running, resiliency for falling, traction for turning, and a flat, highly uniform field.…

  10. Energy Consumption of Battery-Operated Screwdriver at Assembling Fixed Threaded Joints / Akumulatora Skrūvgrieža Elektroenerģijas Patēriņa Aprēķins, Veicot Nekustīga Vītņu Savienojuma Salikšanu Atkarībā No Operatora Reakcijas Laika

    NASA Astrophysics Data System (ADS)

    Grinevich, I.; Mozga, N.; Rudzitis, J.

    2013-10-01

    The electrical energy consumption of battery-operated screwdrivers, which are widely used in many industries, e.g. automotive, heavy, chemical, etc., can be considerably reduced. This would allow increasing the service life of a battery and reducing the time of its charging, thus increasing the productivity and decreasing the prime cost of this battery. It is shown that up to 17.9% of electrical energy could be saved at assembling fixed threaded joints by removing power from the screwdriver immediately after reaching the required tightening torque. Dotajā rakstā uzmanība tiek pievērsta akumulatora skrūvgrieža elektroenerģijas patēriņa samazināšanas iespējām sakarā ar problēmas aktualitāti šīs grupas plaši pielietotajiem instrumentiem. Elektroenerģijas patēriņa samazināšana masveida ražošanā, kur tiek izmantoti akumulatora skrūvgrieži, ļautu paildzināt akumulatora baterijas kalpošanas mūžu un samazināt uzlādēšanas laiku, kas, savukārt, novestu pie ražības palielināšanas un produkcijas pašizmaksas samazināšanas.

  11. Howard Coble Coast Guard and Maritime Transportation Act of 2014

    THOMAS, 113th Congress

    Rep. Hunter, Duncan D. [R-CA-50

    2014-12-01

    12/04/2014 Received in the Senate and Read twice and referred to the Committee on Commerce, Science, and Transportation. (All Actions) Notes: For further action, see S.2444, which became Public Law 113-281 on 12/18/2014. Tracker: This bill has the status Passed HouseHere are the steps for Status of Legislation:

  12. Institutional Indicators: Proposed Budget, 1985-1986. Howard Community College.

    ERIC Educational Resources Information Center

    Radcliffe, Susan K.

    This report provides institutional indicators for budget projections based on data on student characteristics, credit instruction and degrees, credit-free instruction, faculty, community use of facilities, and fiscal background for fiscal years (FY) 1980 through 1984. Part 1 provides data on enrollments by program type, gender, minority status,…

  13. Bone mass in physicians: a Howard University Hospital pilot study.

    PubMed Central

    Reyes, May O.; Archer, Juanita A.; Nunlee-Bland, Gail; Daniel, Gilbert; Morgan, Odette A.; Makambi, Kepher

    2004-01-01

    PURPOSE: This observational cross-sectional study was done to determine bone mass in physicians and to determine if variables, such as calcium intake and exercise, were related to their bone mass. METHODS: One-hundred physicians of different ethnicities (African, African American, Asian, Caribbean, and Hispanic) were studied. Using dual-energy x-ray absorptiometry (DEXA), bone mass (BMD) of the lumbar spine and hips was measured. A validated questionnaire was used to determine the daily calcium intake and exercise. Student t-test, logistic regression, and Pearson chi-square were used to analyze the data. RESULTS: The study population consisted of 52% men and 48% women, with a mean age of 42 years old and a body mass index of 18.5 to 39.9 kg/m2. Low BMD occurred in 68% of the physicians (osteoporosis in 12%, osteopenia in 56%). Low calcium intake was found in 71%-14% of whom had osteoporosis and 49% osteopenia. Two-thirds of the physicians had inadequate exercise; 57% of this group had decreased BMD (osteoporosis in 9%, osteopenia in 38%). There was no statistical significance between BMD and calcium intake or exercise. CONCLUSION: A high percentage of the physicians in this unique study had a reduced BMD. Most of the physicians with low BMD were less than 45 years of age. This study indicates the need to define BMD in a larger cohort of young, ethnically diverse clinicians, and other health workers. Images Figure 1 Figure 2 PMID:15040511

  14. Peter Howard Elworthy (1933-1995): a biographical note.

    PubMed

    Florence, A T

    1997-06-01

    Peter Elworthy had a considerable influence on pharmaceutical science, education and practice, in the UK. He died in December 1995 at the early age of 62, but he had retired from full time academic work from his post as Professor of Pharmacy and Head of the Department of Pharmacy in the University of Manchester twelve years earlier. Concerned about his health but also not a little disillusioned by the multiple pressures placed even then on senior academics, he foresaw the era of cuts and the central oversight and restrictions. His heart was elsewhere. In his 1976 Harrison Memorial Lecture (reproduced in this issue) he said: "Looking back, I have the feeling of having been very lucky. 'Much have I travell'd in the realms of gold, and many goodly states and kingdoms seen.' My main interest has been in the phenomenon of micellization. The subject has been of absorbing interest, and continually shows new bright facets which lead to new scientific advances. Travelling in the realms of gold has nothing to do with gold, but to me it means travelling in sunlight, which illuminates things brightly, and makes visible new facts, which have been invisible before. Occasionally dark clouds form; they are the disappointments and frustrations, but we need them in order to be able to recognize the sunlight by contrast."

  15. Peter Howard Elworthy (1933-1995): a biographical note.

    PubMed

    Florence, A T

    1997-06-01

    Peter Elworthy had a considerable influence on pharmaceutical science, education and practice, in the UK. He died in December 1995 at the early age of 62, but he had retired from full time academic work from his post as Professor of Pharmacy and Head of the Department of Pharmacy in the University of Manchester twelve years earlier. Concerned about his health but also not a little disillusioned by the multiple pressures placed even then on senior academics, he foresaw the era of cuts and the central oversight and restrictions. His heart was elsewhere. In his 1976 Harrison Memorial Lecture (reproduced in this issue) he said: "Looking back, I have the feeling of having been very lucky. 'Much have I travell'd in the realms of gold, and many goodly states and kingdoms seen.' My main interest has been in the phenomenon of micellization. The subject has been of absorbing interest, and continually shows new bright facets which lead to new scientific advances. Travelling in the realms of gold has nothing to do with gold, but to me it means travelling in sunlight, which illuminates things brightly, and makes visible new facts, which have been invisible before. Occasionally dark clouds form; they are the disappointments and frustrations, but we need them in order to be able to recognize the sunlight by contrast." PMID:9330193

  16. NASA/Howard University Large Space Structures Institute

    NASA Technical Reports Server (NTRS)

    Broome, T. H., Jr.

    1984-01-01

    Basic research on the engineering behavior of large space structures is presented. Methods of structural analysis, control, and optimization of large flexible systems are examined. Topics of investigation include the Load Correction Method (LCM) modeling technique, stabilization of flexible bodies by feedback control, mathematical refinement of analysis equations, optimization of the design of structural components, deployment dynamics, and the use of microprocessors in attitude and shape control of large space structures. Information on key personnel, budgeting, support plans and conferences is included.

  17. Howard Florey, Alexander Fleming and the fairy tale of penicillin.

    PubMed

    Goldsworthy, Peter D; McFarlane, Alexander C

    2002-02-18

    The public myth of the discovery of penicillin is an archetypal "quest story" of the type common to every human culture. But the real story of the discovery, testing and refinement of penicillin is a complex tale of accident, serendipity, oversight, conflict, the pressure of war, idiosyncratic personalities and even--the invention of history.

  18. [Activities of the Department of Electrical Engineering, Howard University

    NASA Technical Reports Server (NTRS)

    Yalamanchili, Raj C.

    1997-01-01

    Theoretical derivations, computer analysis and test data are provided to demonstrate that the cavity model is a feasible one to analyze thin-substrate, rectangular-patch microstrip antennas. Seven separate antennas were tested. Most of the antennas were designed to resonate at L-band frequencies (1-2 GHz). One antenna was designed to resonate at an S-band (2-4 GHz) frequency of 2.025 GHz. All dielectric substrates were made of Duroid, and were of varying thicknesses and relative dielectric constant values. Theoretical derivations to calculate radiated free space electromagnetic fields and antenna input impedance were performed. MATHEMATICA 2.2 software was used to generate Smith Chart input impedance plots, normalized relative power radiation plots and to perform other numerical manipulations. Network Analyzer tests were used to verify the data from the computer programming (such as input impedance and VSWR). Finally, tests were performed in an anechoic chamber to measure receive-mode polar power patterns in the E and H planes. Agreement between computer analysis and test data is presented. The antenna with the thickest substrate (e(sub r) = 2.33,62 mils thick) showed the worst match to theoretical impedance data. This is anticipated due to the fact that the cavity model generally loses accuracy when the dielectric substrate thickness exceeds 5% of the antenna's free space wavelength. A method of reducing computer execution time for impedance calculations is also presented.

  19. The Howard Era--In Retrospect? Border Control

    ERIC Educational Resources Information Center

    Brett, Judith

    2004-01-01

    The last eight years have created radical realignments in Australia's political landscape. The Prime Minister's bitterest enemies are precisely the same people who would once have been Australian Liberalism's stalwarts. The author writes on the legacy of Australia's culture wars. For responses, see EJ848173: "Aussie Battler, or Worldly…

  20. The ethical mind. A conversation with psychologist Howard Gardner.

    PubMed

    Gardner, Howard

    2007-03-01

    Business leadership has become synonymous in the public eye with unethical behavior. Widespread scandals, massive layoffs, and inflated executive pay packages have led many to believe that corporate wrongdoing is the status quo. That's why it's more important than ever that those at the top mend relationships with customers, employees, and other stakeholders. Professor Gardner has spent many years studying the relationship between psychology and ethics at Harvard's Graduate School of Education. In this interview with HBR senior editor Bronwyn Fryer, Gardner talks about what he calls the ethical mind, which helps individuals aspire to do good work that matters to their colleagues, companies, and society in general. In an era when workers are overwhelmed by too much information and feel pressured to win at all costs, Gardner believes, it's easy to lose one's way. What's more, employees look to leaders for cues as to what's appropriate and what's not. So if you're a leader, what's the best way to stand up to ethical pressures and set a good example? First and foremost, says Gardner, you must believe that retaining an ethical compass is essential to the health of your organization. Then you must state your ethical beliefs and stick to them. You should also test yourself rigorously to make sure you're adhering to your values, take time to reflect on your beliefs, find multiple mentors who aren't afraid to speak truth to your power, and confront others' egregious behavior as soon as it arises. In the end, Gardner believes, the world hangs in the balance between right and wrong, good and bad, success and disaster. "You need to decide which side you're on:" he concludes, "and do the right thing."

  1. Blood and Gore, Fens and Fears: "Beowulf" Battles the Dale-Chall Monster.

    ERIC Educational Resources Information Center

    Wagner, Betty Jane

    Unfortunately, textbooks of the elementary and junior high school levels receive very little of the honest, critical evaluation which is given trade books in children's literature. One vivid example of this fact was seen in the recent arduous but successful attempt to include "Beowul" in a fourth-grade literature text which was being compiled.…

  2. Step-by-Step Activities to Accompany The Dale Avenue Performance Objectives Model.

    ERIC Educational Resources Information Center

    Schenberg, Rhoda; And Others

    This kit contains performance objectives (stated in behavioral terms) for several areas of school-related skills and suggested activities and materials for teaching those skills to young children. The areas included are: (1) listening, (2) naming, (3) observing, (4) speaking, (5) writing and motor skills, (6) perceptual motor skills, (7)…

  3. Dale Avenue School Early Childhood Education Center Project. Research Bulletin Volume III, No. 2, June 1973.

    ERIC Educational Resources Information Center

    Paterson Board of Education, NJ.

    Reported are results of an evaluation of the handwriting skills of first, second, and third level students who were part of an urban early childhood education project for culturally disadvantaged children in Paterson, New Jersey. Provided is a summary of A. Gesell and F. Ilg's recommendations for handwriting instruction for kindergarten through…

  4. "13 Angry Men": Dale Bumper's "Ad Hominem" Impeachment Trial of President Clinton.

    ERIC Educational Resources Information Center

    Brovero, Adrienne F.

    2000-01-01

    Analyzes Senator Bumpers' argument in the Clinton impeachment proceedings as a bias type of "ad hominem" argument. Notes specifically Bumpers' argument that the House Managers were not fulfilling their proper roles in the proceedings. Claims Bumpers' bias position was reasonable in the argumentative context in which it took place. (NH)

  5. Conversations with Four Highly Productive Educational Psychologists: Patricia Alexander, Richard Mayer, Dale Schunk, and Barry Zimmerman

    ERIC Educational Resources Information Center

    Patterson-Hazley, Melissa; Kiewra, Kenneth A.

    2013-01-01

    This article seeks to answer the questions: Who are the most productive and influential educational psychologists? What factors characterize these educational psychologists? And, what advice might they pass along to budding scholars? To determine the top educational psychologists, we surveyed the membership of Division 15 (Educational Psychology)…

  6. Carbonate chemistry of surface waters in a temperate karst region: the southern Yorkshire Dales, UK

    NASA Astrophysics Data System (ADS)

    Pentecost, Allan

    1992-11-01

    A detailed study of surface water chemistry is described from an important limestone region in northern England. Major ions and pH were determined for 485 sites (springs, seeps, streams, rivers and lakes) during summertime. The saturation state of the waters with respect to calcite was determined as the calcite saturation ratio (Ω). An unexpectedly large number of samples were found to be supersaturated (65.5% of the 268 km of watercourses surveyed). As a consequence, several streams entering major cave systems were incapable of further limestone solution, at least during periods of low flow. Many waters were supersaturated from their source and some deposited travertine. A significant negative correlation was found between spring discharge and both (Ω) and pH. Supersaturation was caused primarily by atmospheric degassing, with some contribution from aquatic plant photosynthesis. The median total dissolved inorganic carbon and Ca concentrations were 2.49 and 1.35 millimoles 1 -1 respectively. Calcium originated exclusively from limestone, and carbon dioxide mainly from the soil and dissolved limestone. South facing catchments provided springwaters with significantly higher levels of TDIC and Ca when compared with north facing catchments. The study suggests that acid rain made a measurable contribution to limestone dissolution. Carboniferous limestone denudation rates were estimated as 54 to 63 m 3 km -2 a -1 (54 to 63 mm 1000 years -1). About 50% of the Mg came from limestone and the remainder, together with most K, Na, SO 4 and Cl from precipitation. Concentrations of dissolved nutrients were low, medians for NO 3, NH 4, total PO 4 and SiO 3 were 24 μmol, 1.4 μmol, 0.64 μmol and 15.5 μmol 1 -1 respectively. The concentration of a further 23 trace elements was determined.

  7. STS-8 onboard crew press conference

    NASA Technical Reports Server (NTRS)

    1983-01-01

    Six news reporters listen to a response from Astronaut Guion S. Bluford (note TV monitor) in a rare space-to-Earth press conference involving all the STS-8 crew. The participants are, left to right, Gary Schwitzer, Cable News Network; Morton Dean, CBS; Roy Neal, NBC; Lynn Sherr, ABC; Howard Benedict, Associated Press; Al Rossiter, United Press International. The astronauts on the monitor are Richard H. Truly, cneter left, crew commander; Daniel C. Brandenstein, lower left, pilot; and Dr. William E. Thornton, upper left, Guion S. Bluford, upper right; and Dale E. Gardner, all mission specialists.

  8. PAT: an intelligent authoring tool for facilitating clinical trial design.

    PubMed

    Tagaris, Anastasios; Andronikou, Vassiliki; Karanastasis, Efstathios; Chondrogiannis, Efthymios; Tsirmpas, Charalambos; Varvarigou, Theodora; Koutsouris, Dimitris

    2014-01-01

    Great investments are made by both private and public funds and a wealth of research findings is published, the research and development pipeline phases quite low productivity and tremendous delays. In this paper, we present a novel authoring tool which has been designed and developed for facilitating study design. Its underlying models are based on a thorough analysis of existing clinical trial protocols (CTPs) and eligibility criteria (EC) published in clinicaltrials.gov by domain experts. Moreover, its integration with intelligent decision support services and mechanisms linking the study design process with healthcare patient data as well as its direct access to literature designate it as a powerful tool offering great support to researchers during clinical trial design.

  9. ML-PAT. Mohawk Language Picture Association Test.

    ERIC Educational Resources Information Center

    Cole, Glory; And Others

    This picture association test booklet for the Mohawk language has two parts. Part One contains 10 questions and Part Two contains 40 questions. Each item consists of a word in Mohawk and a number of drawing from which the learner is to choose the correct one that represents the word. (AMH)

  10. 'All I Can Remember Were Tablets': Pat's Story.

    ERIC Educational Resources Information Center

    McClimens, Alex

    2002-01-01

    This article discusses the use of personal narrative in social science research and in helping individuals with learning difficulties renegotiate their sense of identity. One woman's story of her life is used to illustrate the corrosive effects of institutionalization in identity formation. (Contains references.) (DB)

  11. It's All about the Money: Chris and Pat Compare Salaries

    ERIC Educational Resources Information Center

    Renard, Monika K.

    2008-01-01

    Can you name 20 influences on pay that could cause a difference in earnings between two ostensibly equal employees? This short, involving exercise can be used to illustrate the numerous influences that affect how employees' pay is determined, for example, education, experience required for the job, supply and demand, company size, seniority, and…

  12. Compliance Monitoring of Juvenile Yearling Chinook Salmon and Steelhead Survival and Passage at The Dales Dam, Spring 2011

    SciTech Connect

    Skalski, John R.; Townsend, Richard L.; Seaburg, Adam; Johnson, Gary E.; Ploskey, Gene R.; Carlson, Thomas J.

    2012-02-01

    The study estimated dam passage survival at The Dalles Dam as stipulated by the 2008 Federal Columbia River Power System (FCRPS) Biological Opinion (BiOp) and provided additional performance measures as stipulated in the Columbia Basin Fish Accords. This summary report focuses on spring run stocks, yearling Chinook salmon and steelhead.

  13. Particle size and concentration adjustments of tomato products for Howard mold count.

    PubMed

    Bandler, R; Cichowicz, S M; Cichowicz, S; Floyd, D; Kaminski, J; Russell, G; Senff, W; Trauba, R

    1981-05-01

    The present AOAC method for mold counts of tomato products (44.096) provides no inter-product standardization of concentration among juice, sauce, paste, puree, and catsup; no intra-product standardization of concentration for juice, sauce, and catsup; and no adjustment for degree of comminution. Use of the official method, therefore, could result in an artificially increased mold count for products which have undergone extreme comminution. A new method was developed to adjust all products to the same concentration and grind them to a uniform particle size, thereby ensuring comparable mold counts on products produced by different processes. Collaborative study results showed equal repeatability for both the official and the proposed methods and a lower coefficient of variation for the proposed method.

  14. Why Baby Why: Howard Broomfield's Documentation of the Dunne-za Soundscape.

    ERIC Educational Resources Information Center

    Ridington, Robin

    1988-01-01

    Examines the acoustic environment and aural culture of the Dunne-za of northeastern British Columbia. Discusses the changing soundscape of a northern hunting people, techniques for recording and cataloging an ethnographic soundscape archive, and the use of audio actualities in producing ethnographic audio documentaries. (Author/SV)

  15. Howard Gardner, Meet Benjamin Bloom: Strategies for the Future Enliven Methods from the Past.

    ERIC Educational Resources Information Center

    Savitz, Fred

    Classic theories in pedagogy such as those of John Dewey and Jean Piaget establish the foundation upon which preservice students in a social studies methods course build new knowledge about the teaching/learning paradigm. The constructivist philosophy emanating from these theories provides the rationale for a technique designed to enable students…

  16. "One Long Struggle for Justice": An Interview with Historian Howard Zinn

    ERIC Educational Resources Information Center

    Bigelow, Bill

    2010-01-01

    In 2008 "Rethinking Schools" and the Washington, D.C.-based education nonprofit Teaching for Change joined together to form the Zinn Education Project, dedicated to promoting the teaching of a people's history in middle and high schools throughout the United States. The Zinn Education Project recently launched a new website, www.zinnedproject.org,…

  17. Lucy Diggs Slowe, Howard University Dean of Women, 1922-1937: Educator Administrator, Activist

    ERIC Educational Resources Information Center

    Rasheed, Lisa R.

    2010-01-01

    Within the last twenty years, some educational researchers initiated an emphasis to study the accomplishments and contributions of African-American women in higher education. Although they were marginally recognized, some African-American women forged into uncharted territories by providing examples of administrative leadership in post-secondary…

  18. 75 FR 18826 - Mr. Howard Rosenfeld; Notice of Preliminary Permit Application Accepted for Filing and Soliciting...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-04-13

    ... Project (Warren Project), to be located on Sucker Brook (a.k.a. Lake Waramaug Brook) in Litchfield County... second (cfs) and total installed generating capacity of 10 kilowatts (kW); (6) a new approximately 400-foot-long, 3.4 kilovolt (kV) transmission line, which would connect with the existing 23...

  19. Howard Zinn and the Struggle for the Microphone: History, Objectivity, and Citizenship

    ERIC Educational Resources Information Center

    Kelly, Jason M.

    2009-01-01

    Every year, historians in the United States attend the American Historical Association (AHA), a conference that has met annually since 1884. The AHA draws scholars from all specializations, and it is the primary organization through which the profession is represented. In 1969, the conference met at the Sheraton Park Hotel in Washington, D.C. At…

  20. Howard P. "Buck" McKeon National Defense Authorization Act for Fiscal Year 2015

    THOMAS, 113th Congress

    Rep. McKeon, Howard P. "Buck" [R-CA-25

    2014-04-09

    06/05/2014 Received in the Senate. Read twice. Placed on Senate Legislative Calendar under General Orders. Calendar No. 425. (All Actions) Notes: For further action, see H.R.3979, which became Public Law 113-291 on 12/19/2014. Tracker: This bill has the status Passed HouseHere are the steps for Status of Legislation:

  1. Accreditation. Chassin and Joint Commission aim to inspire. Interview by Howard Larkin.

    PubMed

    Chassin, Mark; Conway, James B; Umbdenstock, Richard J; Dwyer, James; Langberg, Michael L; Petasnick, William D

    2010-03-01

    After two years at the helm of the Joint Commission, Mark Chassin, M.D., is pressing forward with efforts to turn the Joint Commission into a partner with hospitals and other providers and transform health care into a "high reliability" enterprise along the lines of the aviation and nuclear energy industries. Hospitals & Health Networks asked health care experts and Chassin himself how he's doing so far. PMID:20377090

  2. Treatment of PAT. Bradycardiac reflexes induced by dive vs. body-tilt.

    PubMed

    Arabian, J M; Furedy, J J; Morrison, J; Szalai, J P

    1983-01-01

    It has been suggested that the dive reflex (elicited by face immersion in water with breath-hold) may be employed to induce vagally-mediated bradycardia in individuals with paroxysmal atrial tachycardia. However, in several cases the use of this reflex is reported to have led to exacerbation of the cardiac dysfunction. Examination of heart-rate and EKG T-wave amplitude changes during the dive in healthy, college-aged individuals indicated that warming the water (10 degrees through 20 degrees C to 40 degrees C) decreased the bradycardia, but did not alter the attenuation (approximately 100 mu v) of the T-wave. The latter effect was taken to indicate that sympathetic activity, as indexed by T-wave amplitude, was not reduced by increases in water temperature. On the other hand, a 90 degrees head-up to head-down body tilt produced a bradycardia response of some 30 bpm as well as a T-wave amplitude increase (sympathetic withdrawal) in the order of 100 mu v. These data suggest that individuals who exhibit symptoms of ventricular sympathetic irritability may be adversely affected by the dive maneuver, regardless of water temperature. The body-tilt induced reflex in such individuals may thus be more appropriate given the large-magnitude bradycardia and absence of T-wave attenuation. At the present, however, the body-tilt preparation has been used only with healthy normal subjects, so this suggestion remains to be directly tested.

  3. Design and Preliminary Testing of a High Performance Antiproton Trap (HiPAT)

    NASA Technical Reports Server (NTRS)

    Martin, James; Meyer, Kirby; Kramer, Kevin; Smith, Gerald; Lewis, Raymond; Rodgers, Stephen L. (Technical Monitor)

    2000-01-01

    Antimatter represents the pinnacle of energy density, offering the potential to enhance current fusion/fission concepts enabling various classes of deep space missions. Current production rates are sufficient to support proof-of-concept evaluation of many key technologies associated with antimatter-derived propulsion. Storage has been identified as a key enabling technology for all antimatter-related operations, and as such is the current focus of this NASA-MSFC effort to design and fabricate a portable device capable of holding up to 10(exp 12) particles. Hardware has been assembled and initial tests are underway to evaluate the trap behavior using electron gun generated, positive hydrogen ions. Ions have been stored for tens of minutes, limited by observed interaction with background gas. Additionally, radio frequency manipulation is being tested to increase lifetime by stabilizing the stored particles, potentially reducing their interaction with background gas, easing requirements on ultimate trap vacuum and precision mechanical alignment.

  4. Is She Patting Katie? Constraints on Pronominal Reference in 30-Month-Olds

    ERIC Educational Resources Information Center

    Lukyanenko, Cynthia; Conroy, Anastasia; Lidz, Jeffrey

    2014-01-01

    In this study we investigate young children's knowledge of syntactic constraints on Noun Phrase reference by testing 30-month-olds' interpretation of two types of transitive sentences. In a preferential looking task, we find that children prefer different interpretations for transitive sentences whose object NP is a name (e.g.,…

  5. Multivariate data analysis as a PAT tool for early bioprocess development data.

    PubMed

    Mercier, Sarah M; Diepenbroek, Bas; Dalm, Marcella C F; Wijffels, Rene H; Streefland, Mathieu

    2013-09-10

    Early development datasets are typically unstructured, incomplete and truncated, yet they are readily available and contain relevant process information which is not extracted using classical data analysis techniques. In this paper, we illustrate the power of multivariate data analysis (MVDA) as a Process Analytical Technology tool to analyze early development data of a PER.C6® cell cultivation process. MVDA increased our understanding of the process studied. Principal component analysis enabled a thorough exploration of the dataset, identifying causes for batch deviations and revealing sensitivity of the process to scale. These findings were previously undetected using traditional univariate analysis. The lack of structure and gaps in the early development datasets made it impossible to fit them to more advanced partial least square regression models. This paper clearly shows that MVDA should be routinely used to analyze early development data to reveal relevant information for later development and scale-up. The value of these early development runs can be greatly enhanced if the experiments are well-structured and accompanied with full process analytics. This up-front investment will result in shorter and more efficient process development paths, resulting in lower overall development costs for new biopharmaceutical products.

  6. How Early Events Affect Growing Brains. An Interview with Neuroscientist Pat Levitt

    ERIC Educational Resources Information Center

    National Scientific Council on the Developing Child, 2006

    2006-01-01

    Recent advances in neuroscience show clearly how experience can change brain neurochemicals, and how this in turn affects the way the brain functions. As a result, early negative events actually get built into the growing brain's neurochemistry, altering the brain's architecture. Research is continuing to investigate how children with genetic…

  7. Dung pat nesting by the solitary bee, Osmia (Acanthosmiodes) integra (Megachilidae: Apiformes).

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Solitary bees nest in a diversity of substrates, typically soil, but also wood, stems and twigs. A new and novel substrate is reported here, dried cattle dung. Two species of Osmia bees were found nesting in dung in Wyoming. One species, O. integra, otherwise nests shallowly in soil. Nests were ...

  8. RF Manipulation and Detection of Protons in the High Performance Antiproton Trap (HiPAT)

    NASA Technical Reports Server (NTRS)

    Martin, James J.; Lewis, Raymond A.; Pearson, J. Boise; Sims, W. Herb; Chakrabarti, Suman; Fant, Wallace E.; McDonald, Stan

    2003-01-01

    The significant energy density of matter-antimatter annihilation is attractive to the designers of future space propulsion systems, with the potential to offer a highly compact source of power. Many propulsion concepts exist that could take advantage of matter-antimatter reactions, and current antiproton production rates are sufficient to support basic proof-of-principle evaluation of technology associated with antimatter-derived propulsion. One enabling technology for such experiments is portable storage of low energy antiprotons, allowing antiprotons to be trapped, stored, and transported for use at an experimental facility.

  9. 40 CFR 174.522 - Phosphinothricin Acetyltransferase (PAT); exemption from the requirement of a tolerance.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) PESTICIDE PROGRAMS PROCEDURES AND REQUIREMENTS FOR PLANT...) enzyme are exempt from the requirement of a tolerance when used as plant-incorporated protectant...

  10. 40 CFR 174.522 - Phosphinothricin Acetyltransferase (PAT); exemption from the requirement of a tolerance.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) PESTICIDE PROGRAMS PROCEDURES AND REQUIREMENTS FOR PLANT...) enzyme are exempt from the requirement of a tolerance when used as plant-incorporated protectant...

  11. 40 CFR 174.522 - Phosphinothricin Acetyltransferase (PAT); exemption from the requirement of a tolerance.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) PESTICIDE PROGRAMS PROCEDURES AND REQUIREMENTS FOR PLANT...) enzyme are exempt from the requirement of a tolerance when used as plant-incorporated protectant...

  12. 40 CFR 174.522 - Phosphinothricin Acetyltransferase (PAT); exemption from the requirement of a tolerance.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) PESTICIDE PROGRAMS PROCEDURES AND REQUIREMENTS FOR PLANT...) enzyme are exempt from the requirement of a tolerance when used as plant-incorporated protectant...

  13. 40 CFR 174.522 - Phosphinothricin Acetyltransferase (PAT); exemption from the requirement of a tolerance.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) PESTICIDE PROGRAMS PROCEDURES AND REQUIREMENTS FOR PLANT...) enzyme are exempt from the requirement of a tolerance when used as plant-incorporated protectant...

  14. 75 FR 145 - Clean Air Act Operating Permit Program; Petition for Objection to State Operating Permit for East...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-01-04

    ... Kentucky Power Cooperative, Inc.-- William C. Dale Power Station; Clark County, KY AGENCY: Environmental...) for its William C. Dale Power Station (Dale Station) located in Clark County, Kentucky. This...

  15. 77 FR 20009 - Howard Hughes Medical Institute, et al.; Notice of Consolidated Decision on Applications for Duty...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-04-03

    ...: Electron Microscope. Manufacturer: FEI Company, Czech Republic. Intended Use: See notice at 77 FR 12240... notice at 77 FR 12240, February 29, 2012. Docket Number: 12-004. Applicant: Max Planck Florida Institute.... Intended Use: See notice at 77 FR 12240, February 29, 2012. Docket Number: 12-005. Applicant: VA Palo...

  16. A resolution relative to the death of Howard O. Greene, Jr., former Sergeant at Arms of the United States Senate.

    THOMAS, 113th Congress

    Sen. McConnell, Mitch [R-KY

    2014-11-17

    11/17/2014 Submitted in the Senate, considered, and agreed to without amendment and with a preamble by Unanimous Consent. (consideration: CR S6005; text as passed Senate: CR S6024) (All Actions) Tracker: This bill has the status Passed SenateHere are the steps for Status of Legislation:

  17. 75 FR 63166 - Howard Rosenfeld; Notice of Declaration of Intention and Soliciting Comments, Protests, and/or...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-10-14

    ... Energy Independence Hydroelectric Project will be located on Sucker Brook, a.k.a. Lake Waramaug Brook... existing 22-foot-long, 22-foot-wide, 25-foot-high existing mill building, containing a new 10-kW turbine..., protests, and/or motions filed. k. Description of Project: The proposed Warren Energy...

  18. 77 FR 76457 - Howard Hughes Medical Institute, et al.; Notice of Consolidated Decision on Applications for Duty...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-12-28

    .... Manufacturer: TTP Labtech Ltd., United Kingdom. Intended Use: See notice at 77 FR 70141, November 23, 2012... Use: See notice at 77 FR 70141, November 23, 2012. Comments: None received. Decision: Approved. We.... Intended Use: See notice at 77 FR 70142, November 23, 2012. Comments: None received. Decision: Approved....

  19. Carl Levin and Howard P. "Buck" McKeon National Defense Authorization Act for Fiscal Year 2015

    THOMAS, 113th Congress

    Rep. Barletta, Lou [R-PA-11

    2014-01-31

    12/19/2014 Became Public Law No: 113-291. (TXT | PDF) (All Actions) Notes: See the committee print, containing the legislative text and joint explanatory statement, accompanying the enrolled version of H.R.3979. Tracker: This bill has the status Became LawHere are the steps for Status of Legislation:

  20. Dr. Howard L. Simmons: An Intellectual Biography of the First Black Executive Director of the Middle States Regional Accrediting Body

    ERIC Educational Resources Information Center

    Mendoza, Cynthia

    2009-01-01

    There is limited information on changes to accreditation standards and practices, especially related to diversity. The narrow focus of prior studies and the public's limited understanding of accreditation and its criteria have contributed to this gap in the literature. This qualitative study used intellectual biography as its framework to narrate…

  1. Influence of culture media and environmental factors on mycelial growth and sporulation of Lasiodiplodia theobromae (Pat.) Griffon and Maubl.

    PubMed

    Saha, A; Mandal, P; Dasgupta, S; Saha, D

    2008-05-01

    Lasiodiplodia theobromae, a common tea (Camellia sinensis) pathogen, usually does not sporulate or sporulates poorly in common media, which makes spore production difficult. In this study the effects of culture media, carbon source, nitrogen source, temperature, pH and light on mycelial growth and sporulation were evaluated. Among several carbon sources tested, glucose and sucrose were found superior for growth. Potassium nitrate supplemented media showed maximum growth amongst the tested inorganic nitrogen sources while peptone produced maximum growth among the tested organic nitrogen sources. Tea root extract supplemented potato dextrose agar medium was found to be the most suitable for mycelial growth and sporulation of L. theobromae. The fungus grow at temperatures ranging from 40 to 36 degrees C, with optimum growth at 28 degrees C and no growth was noted at 40 degrees C. There was no significant effect of different light period on growth of L. theobromae, but light enhanced sporulation. The fungus grow at pH 3.0-8.0 and optimum growth was observed at pH 6.0. Tea root extract supplemented potato dextrose agar medium with pH 6.0 was the most suitable for production of conidia of L. theobromae at 28 degrees C. Hence this media may be recommended for inoculum production for further studies.

  2. Morphological characterization of the mycorrhiza formed by Helianthemum almeriense Pau with Terfezia claveryi Chatin and Picoa lefebvrei (Pat.) Maire.

    PubMed

    Gutiérrez, A; Morte, A; Honrubia, M

    2003-12-01

    This work presents the first anatomical description of the mycorrhizal systems of Helianthemum almeriense, and of the structure and ultrastructure of the mycorrhizae formed by this plant species with the ascomycetes Terfezia claveryi and Picoa lefebvrei. Four different mycorrhizal systems are described, the club-shaped mycorrhiza being the most abundant. The type of mycorrhiza formed depended on the mycorrhiza culture conditions, but not on the fungal species. For both fungal species, H. almeriense formed an endomycorrhiza in natural field conditions, an ecto- and ectendomycorrhiza without a sheath in pot cultures, and an ectomycorrhiza with a characteristic sheath and Hartig net in in vitro cultures. This is the first report of a typical sheath in Helianthemum-desert truffle mycorrhizal associations. The results support the idea that culture conditions can induce changes in mycorrhiza morphology and that there is no clear barrier between the two main types of mycorrhiza organization in Helianthemum species. The ultrastructural study confirmed the regular presence of T. claveryi intracellular hyphae in direct contact with the host wall, a localization which seems to be a characteristic of the T. claveryi mycorrhiza organization. The P. lefebvrei mycorrhiza organization was characterized by intracellular hyphae with large amounts of electron-dense globules, probably with a lipidic content, and a warty ornamentation on the wall of the root external hyphae.

  3. Decay of Fecal Indicator Bacterial Populations and Bovine-Associated Source-Tracking Markers in Freshly Deposited Cow Pats

    EPA Science Inventory

    Understanding the survival of fecal indicator bacteria (FIB) and microbial source-tracking (MST) markers is critical to developing pathogen fate and transport models. Although pathogen survival in water microcosms and manure-amended soils is well documented, little is known about...

  4. 'Don't save her'- Sigmund Freud meets Project Pat: the rescue motif in hip-hop.

    PubMed

    Fulmer, Richard H

    2008-08-01

    Freud originally explicated the dynamics of rescue wishes by describing men who fell in love with prostitutes. He saw this attempt at attachment as driven by the man's wish to repay his parents for giving him life. Many subsequent writers shift this emphasis, seeing rescue wishes as motivated by aggressive oedipal competition with the father. This article highlights the attachment aspects of Freud's original conception and traces how writers in the last three decades use the family romance rather than Oedipus as a model to view rescue wishes as having a more tender aspect. Rescue wishes are especially characteristic of the developmental stage of young adulthood. They at once attempt to repay the parental debt the young adult feels and serve as practice for the vicissitudes of the couple bond and the benign sacrifices of parenthood. Popular culture contains many vivid examples of Freud 's original description of the rescue-motif. A detailed examination of the rap song, Don't save her, demonstrates all the elements of Freud's original conception and the interpersonal risks of the rescue relationship. Three additional rap songs by other artists are briefly analyzed to show the extensive occurrence of the wish to rescue and its psychic and interpersonal dangers.

  5. Archiving SLD Records in SRB: The Persistent Archives Test-Bed (PAT) Project at SLAC in 2004

    SciTech Connect

    Deken, J.

    2005-01-26

    Report on the first year of SLAC's participation in the collaboration to test the NARA prototype persistent archives' ability to perform the functions of accessioning, arrangement, description, preservation and access on the electronic records of the SLD (SLAC Large Detector) collaboration.

  6. Peanut Allergen Threshold Study (PATS): validation of eliciting doses using a novel single-dose challenge protocol

    PubMed Central

    2013-01-01

    Background The eliciting dose (ED) for a peanut allergic reaction in 5% of the peanut allergic population, the ED05, is 1.5 mg of peanut protein. This ED05 was derived from oral food challenges (OFC) that use graded, incremental doses administered at fixed time intervals. Individual patients’ threshold doses were used to generate population dose-distribution curves using probability distributions from which the ED05 was then determined. It is important to clinically validate that this dose is predictive of the allergenic response in a further unselected group of peanut-allergic individuals. Methods/Aims This is a multi-centre study involving three national level referral and teaching centres. (Cork University Hospital, Ireland, Royal Children’s Hospital Melbourne, Australia and Massachusetts General Hospital, Boston, U.S.A.) The study is now in process and will continue to run until all centres have recruited 125 participates in each respective centre. A total of 375 participants, aged 1–18 years will be recruited during routine Allergy appointments in the centres. The aim is to assess the precision of the predicted ED05 using a single dose (6 mg peanut = 1.5 mg of peanut protein) in the form of a cookie. Validated Food Allergy related Quality of Life Questionnaires-(FAQLQ) will be self-administered prior to OFC and 1 month after challenge to assess the impact of a single dose OFC on FAQL. Serological and cell based in vitro studies will be performed. Conclusion The validation of the ED05 threshold for allergic reactions in peanut allergic subjects has potential value for public health measures. The single dose OFC, based upon the statistical dose-distribution analysis of past challenge trials, promises an efficient approach to identify the most highly sensitive patients within any given food-allergic population. PMID:24028324

  7. Influence of culture media and environmental factors on mycelial growth and sporulation of Lasiodiplodia theobromae (Pat.) Griffon and Maubl.

    PubMed

    Saha, A; Mandal, P; Dasgupta, S; Saha, D

    2008-05-01

    Lasiodiplodia theobromae, a common tea (Camellia sinensis) pathogen, usually does not sporulate or sporulates poorly in common media, which makes spore production difficult. In this study the effects of culture media, carbon source, nitrogen source, temperature, pH and light on mycelial growth and sporulation were evaluated. Among several carbon sources tested, glucose and sucrose were found superior for growth. Potassium nitrate supplemented media showed maximum growth amongst the tested inorganic nitrogen sources while peptone produced maximum growth among the tested organic nitrogen sources. Tea root extract supplemented potato dextrose agar medium was found to be the most suitable for mycelial growth and sporulation of L. theobromae. The fungus grow at temperatures ranging from 40 to 36 degrees C, with optimum growth at 28 degrees C and no growth was noted at 40 degrees C. There was no significant effect of different light period on growth of L. theobromae, but light enhanced sporulation. The fungus grow at pH 3.0-8.0 and optimum growth was observed at pH 6.0. Tea root extract supplemented potato dextrose agar medium with pH 6.0 was the most suitable for production of conidia of L. theobromae at 28 degrees C. Hence this media may be recommended for inoculum production for further studies. PMID:18972700

  8. Screening of bacterial biocontrols against sapstain fungus (Lasiodiplodia theobromae Pat.) of rubberwood (Hevea brasiliensis Muell.Arg.).

    PubMed

    Sajitha, K L; Maria Florence, E J; Dev, Suma Arun

    2014-09-01

    Diverse bacterial biocontrol agents from various sources of aerobic composts against the sapstain fungus Lasiodiplodia theobromae in rubberwood (Hevea brasiliensis) were isolated, screened and identified by various morphological, biochemical and molecular techniques. The inhibitory effect of seventeen bacterial isolates was examined and seven exhibited inhibition towards the sapstain fungus. Among the seven antagonists, six were conclusively identified as Bacillus subtilis and one as Paenibacillus polymyxa using 16S rRNA-encoding gene sequencing. This is the first report on the occurrence of P. polymyxa, a potent biofertilizer and antagonist in vermicompost. HiCrome Bacillus agar was identified as an effective medium for differentiation of B. subtilis from other Bacillus species. The present work demonstrates the efficacy of the antagonistic property of B. subtilis strains against rubberwood sapstain fungus. Culture-based antagonistic inhibition displayed by B. subtilis can be extended to cater to the biocontrol requirements of wood-based industries against the stain fungus. The study showed the utility of an integrated approach, employing morphological, biochemical and molecular tools for conclusive identification of several bacterial isolates present in aerobic composts from diverse sources. PMID:25049165

  9. A one-dimensional, steady-state, dissolved-oxygen model and waste-load assimilation study for Wildcat Creek, Howard County, Indiana

    USGS Publications Warehouse

    Crawford, Charles G.; Wilber, William G.; Peters, James G.

    1979-01-01

    The Indiana State Board of Health is developing a water-quality management plan that includes establishing limits for wastewater effluents discharged into Indiana streams. A digital model calibrated to conditions in Wildcat Creek was used to predict alternatives for future waste loadings that would be compatible with Indiana stream water-quality standards defined for two critical hydrologic conditions, summer and winter low flows. The model indicates that benthic-oxygen demand is the most significant factor affecting the dissolved-oxygen concentrations in Wildcat Creek during summer low flows. The Indiana stream dissolved-oxygen standard should not be violated if the Kokomo wastewater-treatment facility meets its current National Pollution Discharge Elimination System permit restrictions (average monthly 5-day biochemical-oxygen demand of 5 milligrams per liter and maximum weekly 5-day biochemical-oxygen demand of 7.5 milligrams per liter) and benthic-oxygen demand becomes negligible. Ammonia-nitrogen toxicity may also be a water-quality limitation in Wildcat Creek. Ammonia-nitrogen waste loads for the Kokomo wastewater-treatment facility, projected by the Indiana State Board of Health, will result in stream ammonia-nitrogen concentrations that exceed the State standard (2.5 milligrams per liter during summer months and 4.0 milligrams per liter during winter months). (Kosco-USGS)

  10. Lymphology, medical ignorance/ignoramics and the Nobel connection: Howard Florey, Joshua Lederberg, and Françoise Barre-Sinoussi.

    PubMed

    Witte, M H

    2008-12-01

    Intriguing interludes with Nobel laureates have marked the history of Lymphology. Bounded (or rather unbounded) by a mutual fascination with ignorance and the unknown, their curiosity converged around microbes, infections, and host responses mediated by the lymphatic system. These studies transcended a simple "molecular model of life".

  11. Description of a new species of Anagyrus Howard (Hymenoptera: Chalcidoidea: Encyrtidae), a promising biological control agent of the invasive Madeira mealybug, Phenacoccus madeirensis Green (Hemiptera: Sternorrhyncha: Pseudococcidae).

    PubMed

    Rameshkumar, A; Noyes, J S; Poorani, J; Chong, J H

    2013-01-01

    Anagyrus amnestos sp. n. (Hymenoptera: Encyrtidae), a promising parasitoid of the invasive Madeira mealybug, Phenacoccus madeirensis Green (Hemiptera: Pseudococcidae), is described based on material collected from India. This parasitoid was identified as Anagyrus sp. nov. nr. sinope Noyes & Menezes in recent literature, and was initially collected in Georgia, USA. It was found to be a specific parasitoid of the Madeira mealybug and its biological attributes and potential as a biological control agent of this pest were studied. In what appears to be a case of fortuitous introduction, we detected this parasitoid in large numbers on Madeira mealybugs from the southern Indian state of Karnataka, where the mealybug is a recently introduced invasive pest. In view of its economic importance as a potential biological control agent of the Madeira mealybug, it is formally described and illustrated here. Comparative accounts of the new species vis-a-vis its close relatives in India and the Americas are provided.

  12. Geologic and Fossil Locality Maps of the West-Central Part of the Howard Pass Quadrangle and Part of the Adjacent Misheguk Mountain Quadrangle, Western Brooks Range, Alaska

    USGS Publications Warehouse

    Dover, James H.; Tailleur, Irvin L.; Dumoulin, Julie A.

    2004-01-01

    The map depicts the field distribution and contact relations between stratigraphic units, the tectonic relations between major stratigraphic sequences, and the detailed internal structure of these sequences. The stratigraphic sequences formed in a variety of continental margin depositional environments, and subsequently underwent a complexde formational history of imbricate thrust faulting and folding. A compilation of micro and macro fossil identifications is included in this data set.

  13. Description of a new species of Anagyrus Howard (Hymenoptera: Chalcidoidea: Encyrtidae), a promising biological control agent of the invasive Madeira mealybug, Phenacoccus madeirensis Green (Hemiptera: Sternorrhyncha: Pseudococcidae).

    PubMed

    Rameshkumar, A; Noyes, J S; Poorani, J; Chong, J H

    2013-01-01

    Anagyrus amnestos sp. n. (Hymenoptera: Encyrtidae), a promising parasitoid of the invasive Madeira mealybug, Phenacoccus madeirensis Green (Hemiptera: Pseudococcidae), is described based on material collected from India. This parasitoid was identified as Anagyrus sp. nov. nr. sinope Noyes & Menezes in recent literature, and was initially collected in Georgia, USA. It was found to be a specific parasitoid of the Madeira mealybug and its biological attributes and potential as a biological control agent of this pest were studied. In what appears to be a case of fortuitous introduction, we detected this parasitoid in large numbers on Madeira mealybugs from the southern Indian state of Karnataka, where the mealybug is a recently introduced invasive pest. In view of its economic importance as a potential biological control agent of the Madeira mealybug, it is formally described and illustrated here. Comparative accounts of the new species vis-a-vis its close relatives in India and the Americas are provided. PMID:26176096

  14. Anthropophilic biting behaviour of Anopheles (Kerteszia) neivai Howard, Dyar & Knab associated with Fishermen’s activities in a malaria-endemic area in the Colombian Pacific

    PubMed Central

    Escovar, Jesús Eduardo; González, Ranulfo; Quiñones, Martha Lucía

    2013-01-01

    On the southwest Pacific Coast of Colombia, a field study was initiated to determine the human-vector association between Anopheles (Kerteszia) neivai and fishermen, including their nearby houses. Mosquitoes were collected over 24-h periods from mangrove swamps, marshlands and fishing vessels in three locations, as well as in and around the houses of fishermen. A total of 6,382 mosquitoes were collected. An. neivai was most abundant in mangroves and fishing canoes (90.8%), while Anopheles albimanus was found indoors (82%) and outdoors (73%). One An. neivai and one An. albimanus collected during fishing activities in canoes were positive for Plasmodium vivax , whereas one female An. neivai collected in a mangrove was positive for P. vivax . In the mangroves and fishing canoes, An. neivai demonstrated biting activity throughout the day, peaking between 06:00 pm-07:00 pm and there were two minor peaks at dusk and dawn. These peaks coincided with fishing activities in the marshlands and mangroves, a situation that places the fishermen at risk of contracting malaria when they are performing their daily activities. It is recommended that protective measures be implemented to reduce the risk that fishermen will contract malaria. PMID:24402159

  15. Aeromagnetic survey of Howard Pass quadrangle and the East half of Misheguk Mountain quadrangle, Alaska—a Web site for the distribution of data

    USGS Publications Warehouse

    Brown, Philip J.

    2009-01-01

    U.S. Geological Survey Open-File-Report 2009-1256 is for the preliminary release of magnetic data (and associated contractor reports) for an airborne survey in the Brooks Range, northwest of Bettles, Alaska.

  16. President Franklin D Roosevelt (1882-1945) and Doctor Frank Howard Lahey's (1880-1953) dilemma: The complexities of medical confidentiality with World Leaders.

    PubMed

    Steinberg, David

    2016-02-01

    In the months before the Presidential Election of November 1944, there were serious concerns about the health of President Franklin D Roosevelt. He had lost considerable weight and his once robust appearance had given way to a tired and haggard look. Despite these worrisome observations, Vice-Admiral Ross T McIntire, who served as Franklin Roosevelt's personal physician from 1933 until Roosevelt's death, lied when he proclaimed Roosevelt to be in 'excellent condition for a man of his age'. The truth about Roosevelt's health was further obscured when Roosevelt's medical records disappeared.

  17. On the origin of framboidal pyrite in sediments of the Suakin Deep (Red Sea)Sur l'origine de la pyrite framboïdale dans les sédiments de la fosse Suakin (mer Rouge)

    NASA Astrophysics Data System (ADS)

    Pierret, Marie-Claire; Blanc, Gérard; Clauer, Norbert

    2000-01-01

    Suakin Deep is one of the southern depressions of the Red Sea, with sediments containing up to 20 % of pyrite. Although metalliferous sediments result from hydrothermal activity in most deeps, those of Suakin have different characteristics. Pyrite is framboïdal and the REE patterns of its sediments are similar to those of biodetrital sediments. The sediments seem to be of biodetritic origin and to have undergone diagenetic changes without hydrothermal activity.

  18. Les tourmalinites stratiformesàdravite d'origine colloïdale du Paléoprotérozoïque sénégalo-malien

    NASA Astrophysics Data System (ADS)

    Ndiaye, Papa Moussa; Guillou, Jean Jacques

    1997-04-01

    In the Palaeoproterozoic (2.1 Ga) rocks from Dialé-Daléma, lenses of tourmalinite (dravite) occur in felsic volcaniclastics. They characterise a partially emerged N-S trending area. Dravite rich rocks were frequently eroded and redeposited, forming intraformational conglomerates. They are associated with paralic metasediments, which are partly evaporitic: scapolite marbles and graphitic horizons. The study of the micro- and ultrastructures shows some of the dravite to be syngenetic, as it was originally deposited as a colloid. Boron is probably of hydrothermal origin. Upon reaching the surface, the B-rich fluid probably collected in depressions of palaeorelief near the shore. It was accompanied by silica, alumina and ferrous Fe (anoxic atmosphere). From rare earth studies, and the palaeogeographical relations between the paralic deposits and the tourmalinite lenses, Nasbnd Mg-rich waters of marine origin met these solutions. This mixture formed the dravite: Mg2+ is more abundant than Fe2+. This characterises the borosilicates of both exhalative and sedimentary origin.

  19. Scientific analysis is essential to assess biofuel policy effects: in response to the paper by Kim and Dale on "Indirect land use change for biofuels: Testing predictions and improving analytical methodologies"

    SciTech Connect

    Kline, Keith L; Oladosu, Gbadebo A; Dale, Virginia H; McBride, Allen

    2011-01-01

    Vigorous debate on the effects of biofuels derives largely from the changes in land use estimated using economic models designed mainly for the analysis of agricultural trade and markets. The models referenced for land-use change (LUC) analysis in the U.S. Environmental Protection Agency Final Rule on the Renewable Fuel Standard include GTAP, FAPRI-CARD, and FASOM. To address bioenergy impacts, these models were expanded and modified to facilitate simulations of hypothesized LUC. However, even when models use similar basic assumptions and data, the range of LUC results can vary by ten-fold or more. While the market dynamics simulated in these models include processes that are important in estimating effects of biofuel policies, the models have not been validated for estimating land-use changes and employ crucial assumptions and simplifications that contradict empirical evidence.

  20. Étude expérimentale du dépôt de particules colloïdales en milieu poreux : Influence de l'hydrodynamique et de la salinité

    NASA Astrophysics Data System (ADS)

    Djehiche, Abdelkader; Canseco, Vladimir; Omari, Aziz; Bertin, Henri

    2009-09-01

    This study deals with colloid transport in porous media which applications are found in subsurface water, petroleum engineering or civil engineering. An experimental study of colloidal polystyrene Latex particles deposition in a consolidated porous medium is presented. The influence of ionic strength of the colloid suspension and the flow rate on particle deposition is investigated. We see first that beyond a critical salt concentration, the total collector efficiency increases with the ionic strength. Moreover, such collector efficiency decreases as the flow rate increases according to theory. In other respects, using a γ ray attenuation technique allows us to measure local porosity fluctuation due to particles deposition. By this way deposition kinetics may be followed locally and precisely. Nevertheless when considering the thickness of the adsorbed layer over large scales, obtained results using the γ rays attenuation technique are found in good agreement with those obtained by means of an usual technique especially at latest stages of adsorption process. To cite this article: A. Djehiche et al., C. R. Mecanique 337 (2009).

  1. 13. UNIDENTIFIED CABLESTAYED SUSPENSION BRIDGE WITH TIMBER RAILING OF TWELVE ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    13. UNIDENTIFIED CABLE-STAYED SUSPENSION BRIDGE WITH TIMBER RAILING OF TWELVE PANELS, SHOWING CABLE PATTERN SIMILAR TO E.E. RUNYON'S SUSPENSION BRIDGE PATENTS. THE BLUFF DALE SUSPENSION BRIDGE'S CABLES MAY HAVE ORIGINALLY FOLLOWED THIS PATTERN. ELEVATION VIEW. - Bluff Dale Suspension Bridge, Spanning Paluxy River at County Route 149, Bluff Dale, Erath County, TX

  2. 12. UNIDENTIFIED CABLESTAYED SUSPENSION BRIDGE WITH TIMBER RAILING OF TWELVE ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    12. UNIDENTIFIED CABLE-STAYED SUSPENSION BRIDGE WITH TIMBER RAILING OF TWELVE PANELS, SHOWING CABLE PATTERN SIMILAR TO E.E. RUNYON'S SUSPENSION BRIDGE PATENTS. THE BLUFF DALE SUSPENSION BRIDGE'S CABLES MAY HAVE ORIGINALLY FOLLOWED THIS PATTERN. 3/4 VIEW FROM ABOVE. - Bluff Dale Suspension Bridge, Spanning Paluxy River at County Route 149, Bluff Dale, Erath County, TX

  3. Preparation of Power Distribution System for High Penetration of Renewable Energy Part I. Dynamic Voltage Restorer for Voltage Regulation Pat II. Distribution Circuit Modeling and Validation

    NASA Astrophysics Data System (ADS)

    Khoshkbar Sadigh, Arash

    Part I: Dynamic Voltage Restorer In the present power grids, voltage sags are recognized as a serious threat and a frequently occurring power-quality problem and have costly consequence such as sensitive loads tripping and production loss. Consequently, the demand for high power quality and voltage stability becomes a pressing issue. Dynamic voltage restorer (DVR), as a custom power device, is more effective and direct solutions for "restoring" the quality of voltage at its load-side terminals when the quality of voltage at its source-side terminals is disturbed. In the first part of this thesis, a DVR configuration with no need of bulky dc link capacitor or energy storage is proposed. This fact causes to reduce the size of the DVR and increase the reliability of the circuit. In addition, the proposed DVR topology is based on high-frequency isolation transformer resulting in the size reduction of transformer. The proposed DVR circuit, which is suitable for both low- and medium-voltage applications, is based on dc-ac converters connected in series to split the main dc link between the inputs of dc-ac converters. This feature makes it possible to use modular dc-ac converters and utilize low-voltage components in these converters whenever it is required to use DVR in medium-voltage application. The proposed configuration is tested under different conditions of load power factor and grid voltage harmonic. It has been shown that proposed DVR can compensate the voltage sag effectively and protect the sensitive loads. Following the proposition of the DVR topology, a fundamental voltage amplitude detection method which is applicable in both single/three-phase systems for DVR applications is proposed. The advantages of proposed method include application in distorted power grid with no need of any low-pass filter, precise and reliable detection, simple computation and implementation without using a phased locked loop and lookup table. The proposed method has been verified by simulation and experimental tests under various conditions considering all possible cases such as different amounts of voltage sag depth (VSD), different amounts of point-on-wave (POW) at which voltage sag occurs, harmonic distortion, line frequency variation, and phase jump (PJ). Furthermore, the ripple amount of fundamental voltage amplitude calculated by the proposed method and its error is analyzed considering the line frequency variation together with harmonic distortion. The best and worst detection time of proposed method were measured 1ms and 8.8ms, respectively. Finally, the proposed method has been compared with other voltage sag detection methods available in literature. Part 2: Power System Modeling for Renewable Energy Integration: As power distribution systems are evolving into more complex networks, electrical engineers have to rely on software tools to perform circuit analysis. There are dozens of powerful software tools available in the market to perform the power system studies. Although their main functions are similar, there are differences in features and formatting structures to suit specific applications. This creates challenges for transferring power system circuit models data (PSCMD) between different software and rebuilding the same circuit in the second software environment. The objective of this part of thesis is to develop a Unified Platform (UP) to facilitate transferring PSCMD among different software packages and relieve the challenges of the circuit model conversion process. UP uses a commonly available spreadsheet file with a defined format, for any home software to write data to and for any destination software to read data from, via a script-based application called PSCMD transfer application. The main considerations in developing the UP are to minimize manual intervention and import a one-line diagram into the destination software or export it from the source software, with all details to allow load flow, short circuit and other analyses. In this study, ETAP, OpenDSS, and GridLab-D are considered, and PSCMD transfer applications written in MATLAB have been developed for each of these to read the circuit model data provided in the UP spreadsheet. In order to test the developed PSCMD transfer applications, circuit model data of a test circuit and a power distribution circuit from Southern California Edison (SCE) - a utility company - both built in CYME, were exported into the spreadsheet file according to the UP format. Thereafter, circuit model data were imported successfully from the spreadsheet files into above mentioned software using the PSCMD transfer applications developed for each software. After the SCE studied circuit is transferred into OpenDSS software using the proposed UP scheme and developed application, it has been studied to investigate the impacts of large-scale solar energy penetration. The main challenge of solar energy integration into power grid is its intermittency (i.e., discontinuity of output power) nature due to cloud shading of photovoltaic panels which depends on weather conditions. In order to conduct this study, OpenDSS time-series simulation feature, which is required due to intermittency of solar energy, is utilized. In this study, the impacts of intermittency of solar energy penetration, especially high-variability points, on voltage fluctuation and operation of capacitor bank and voltage regulator is provided. In addition, the necessity to interpolate and resample unequally spaced time-series measurement data and convert them to equally spaced time-series data as well as the effect of resampling time-interval on the amount of error is discussed. Two applications are developed in Matlab to do interpolation and resampling as well as to calculate the amount of error for different resampling time-intervals to figure out the suitable resampling time-interval. Furthermore, an approach based on cumulative distribution, regarding the length for lines/cables types and the power rating for loads, is presented to prioritize which loads, lines and cables the meters should be installed at to have the most effect on model validation.

  4. Application of terahertz pulse imaging as PAT tool for non-destructive evaluation of film-coated tablets under different manufacturing conditions.

    PubMed

    Dohi, Masafumi; Momose, Wataru; Yoshino, Hiroyuki; Hara, Yuko; Yamashita, Kazunari; Hakomori, Tadashi; Sato, Shusaku; Terada, Katsuhide

    2016-02-01

    Film-coated tablets (FCTs) are a popular solid dosage form in pharmaceutical industry. Manufacturing conditions during the film-coating process affect the properties of the film layer, which might result in critical quality problems. Here, we analyzed the properties of the film layer using a non-destructive approach with terahertz pulsed imaging (TPI). Hydrophilic tablets that become distended upon water absorption were used as core tablets and coated with film under different manufacturing conditions. TPI-derived parameters such as film thickness (FT), film surface reflectance (FSR), and interface density difference (IDD) between the film layer and core tablet were affected by manufacturing conditions and influenced critical quality attributes of FCTs. Relative standard deviation of FSR within tablets correlated well with surface roughness. Tensile strength could be predicted in a non-destructive manner using the multivariate regression equation to estimate the core tablet density by film layer density and IDD. The absolute value of IDD (Lateral) correlated with the risk of cracking on the lateral film layer when stored in a high-humidity environment. Further, in-process control was proposed for this value during the film-coating process, which will enable a feedback control system to be applied to process parameters and reduced risk of cracking without a stability test.

  5. Prenatal diagnosis of holoprosencephaly in two fetuses with der (7)t(1;7)(q32;q32)pat inherited from the father with double translocations.

    PubMed

    Chuang, Louise; Kuo, Pao-Lin; Yang, Hsiao-Bai; Chien, Chung-Ho; Chen, Pei-Yi; Chang, Chiung-Hsin; Chang, Fong-Ming

    2003-02-01

    The presence of two independent translocations in one person is rare. Herein, we report the prenatal diagnosis of two sibling fetuses with holoprosencephaly, whose father is a carrier of double translocations. The karyotype of the father is 46,XY, t(1;7) (q32;q32), t(14,15) (q32.1;q26.3). The two fetuses had variable facial dysmorphisms and identical cytogenetic abnormality-a derivative (7) t(1;7) (q32;q32) inherited from the father. The proband 1 showed a small mouth, a single median eye and a proboscis above the eye, while the proband 2 showed hypotelorism, a flat nose, cleft lip and cleft palate. Both fetuses also had alobar holoprosencephaly. Haploinsufficiency of the sonic hedgehog gene at 7q36 does account for the occurrence of holoprosencephaly in the two fetuses with a deletion of distal 7q (7q32 --> qter). PMID:12575020

  6. Preparation of Power Distribution System for High Penetration of Renewable Energy Part I. Dynamic Voltage Restorer for Voltage Regulation Pat II. Distribution Circuit Modeling and Validation

    NASA Astrophysics Data System (ADS)

    Khoshkbar Sadigh, Arash

    Part I: Dynamic Voltage Restorer In the present power grids, voltage sags are recognized as a serious threat and a frequently occurring power-quality problem and have costly consequence such as sensitive loads tripping and production loss. Consequently, the demand for high power quality and voltage stability becomes a pressing issue. Dynamic voltage restorer (DVR), as a custom power device, is more effective and direct solutions for "restoring" the quality of voltage at its load-side terminals when the quality of voltage at its source-side terminals is disturbed. In the first part of this thesis, a DVR configuration with no need of bulky dc link capacitor or energy storage is proposed. This fact causes to reduce the size of the DVR and increase the reliability of the circuit. In addition, the proposed DVR topology is based on high-frequency isolation transformer resulting in the size reduction of transformer. The proposed DVR circuit, which is suitable for both low- and medium-voltage applications, is based on dc-ac converters connected in series to split the main dc link between the inputs of dc-ac converters. This feature makes it possible to use modular dc-ac converters and utilize low-voltage components in these converters whenever it is required to use DVR in medium-voltage application. The proposed configuration is tested under different conditions of load power factor and grid voltage harmonic. It has been shown that proposed DVR can compensate the voltage sag effectively and protect the sensitive loads. Following the proposition of the DVR topology, a fundamental voltage amplitude detection method which is applicable in both single/three-phase systems for DVR applications is proposed. The advantages of proposed method include application in distorted power grid with no need of any low-pass filter, precise and reliable detection, simple computation and implementation without using a phased locked loop and lookup table. The proposed method has been verified by simulation and experimental tests under various conditions considering all possible cases such as different amounts of voltage sag depth (VSD), different amounts of point-on-wave (POW) at which voltage sag occurs, harmonic distortion, line frequency variation, and phase jump (PJ). Furthermore, the ripple amount of fundamental voltage amplitude calculated by the proposed method and its error is analyzed considering the line frequency variation together with harmonic distortion. The best and worst detection time of proposed method were measured 1ms and 8.8ms, respectively. Finally, the proposed method has been compared with other voltage sag detection methods available in literature. Part 2: Power System Modeling for Renewable Energy Integration: As power distribution systems are evolving into more complex networks, electrical engineers have to rely on software tools to perform circuit analysis. There are dozens of powerful software tools available in the market to perform the power system studies. Although their main functions are similar, there are differences in features and formatting structures to suit specific applications. This creates challenges for transferring power system circuit models data (PSCMD) between different software and rebuilding the same circuit in the second software environment. The objective of this part of thesis is to develop a Unified Platform (UP) to facilitate transferring PSCMD among different software packages and relieve the challenges of the circuit model conversion process. UP uses a commonly available spreadsheet file with a defined format, for any home software to write data to and for any destination software to read data from, via a script-based application called PSCMD transfer application. The main considerations in developing the UP are to minimize manual intervention and import a one-line diagram into the destination software or export it from the source software, with all details to allow load flow, short circuit and other analyses. In this study, ETAP, OpenDSS, and GridLab-D are considered, and PSCMD trans

  7. Application of terahertz pulse imaging as PAT tool for non-destructive evaluation of film-coated tablets under different manufacturing conditions.

    PubMed

    Dohi, Masafumi; Momose, Wataru; Yoshino, Hiroyuki; Hara, Yuko; Yamashita, Kazunari; Hakomori, Tadashi; Sato, Shusaku; Terada, Katsuhide

    2016-02-01

    Film-coated tablets (FCTs) are a popular solid dosage form in pharmaceutical industry. Manufacturing conditions during the film-coating process affect the properties of the film layer, which might result in critical quality problems. Here, we analyzed the properties of the film layer using a non-destructive approach with terahertz pulsed imaging (TPI). Hydrophilic tablets that become distended upon water absorption were used as core tablets and coated with film under different manufacturing conditions. TPI-derived parameters such as film thickness (FT), film surface reflectance (FSR), and interface density difference (IDD) between the film layer and core tablet were affected by manufacturing conditions and influenced critical quality attributes of FCTs. Relative standard deviation of FSR within tablets correlated well with surface roughness. Tensile strength could be predicted in a non-destructive manner using the multivariate regression equation to estimate the core tablet density by film layer density and IDD. The absolute value of IDD (Lateral) correlated with the risk of cracking on the lateral film layer when stored in a high-humidity environment. Further, in-process control was proposed for this value during the film-coating process, which will enable a feedback control system to be applied to process parameters and reduced risk of cracking without a stability test. PMID:26678177

  8. Real-time polymerase chain reaction-based approach for quantification of the pat gene in the T25 Zea mays event.

    PubMed

    Weighardt, Florian; Barbati, Cristina; Paoletti, Claudia; Querci, Maddalena; Kay, Simon; De Beuckeleer, Marc; Van den Eede, Guy

    2004-01-01

    In Europe, a growing interest for reliable techniques for the quantification of genetically modified component(s) of food matrixes is arising from the need to comply with the European legislative framework on novel food products. Real-time polymerase chain reaction (PCR) is currently the most powerful technique for the quantification of specific nucleic acid sequences. Several real-time PCR methodologies based on different molecular principles have been developed for this purpose. The most frequently used approach in the field of genetically modified organism (GMO) quantification in food or feed samples is based on the 5'-3'-exonuclease activity of Taq DNA polymerase on specific degradation probes (TaqMan principle). A novel approach was developed for the establishment of a TaqMan quantification system assessing GMO contents around the 1% threshold stipulated under European Union (EU) legislation for the labeling of food products. The Zea mays T25 elite event was chosen as a model for the development of the novel GMO quantification approach. The most innovative aspect of the system is represented by the use of sequences cloned in plasmids as reference standards. In the field of GMO quantification, plasmids are an easy to use, cheap, and reliable alternative to Certified Reference Materials (CRMs), which are only available for a few of the GMOs authorized in Europe, have a relatively high production cost, and require further processing to be suitable for analysis. Strengths and weaknesses of the use of novel plasmid-based standards are addressed in detail. In addition, the quantification system was designed to avoid the use of a reference gene (e.g., a single copy, species-specific gene) as normalizer, i.e., to perform a GMO quantification based on an absolute instead of a relative measurement. In fact, experimental evidences show that the use of reference genes adds variability to the measurement system because a second independent real-time PCR-based measurement must be performed. Moreover, for some reference genes no sufficient information on copy number in and among genomes of different lines is available, making adequate quantification difficult. Once developed, the method was subsequently validated according to IUPAC and ISO 5725 guidelines. Thirteen laboratories from 8 EU countries participated in the trial. Eleven laboratories provided results complying with the predefined study requirements. Repeatability (RSDr) values ranged from 8.7 to 15.9%, with a mean value of 12%. Reproducibility (RSDR) values ranged from 16.3 to 25.5%, with a mean value of 21%. Following Codex Alimentarius Committee guidelines, both the limits of detection and quantitation were determined to be <0.1%.

  9. How HANDy Are Baby Signs? A Systematic Review of the Impact of Gestural Communication on Typically Developing, Hearing Infants under the Age of 36 Months: Response to Howard and Doherty-Sneddon's Commentary

    ERIC Educational Resources Information Center

    Fitzpatrick, Elizabeth M.; Johnston, J. Cyne; Thibert, Jonelle; Grandpierre, Viviane

    2014-01-01

    A systematic review was conducted to synthesize the evidence related to the effectiveness of baby sign language for children with typical development. This response to a Commentary on the review stresses that the primary purpose of the review was to assist caregivers and policy makers with informed decision-making related to the benefits of the…

  10. Ecology of Alcohol and Other Drug Use: Helping Black High-Risk Youth. Proceedings of the Howard University School of Human Ecology Forum (Washington, D.C., October 26-27, 1987). OSAP Prevention Monograph-7.

    ERIC Educational Resources Information Center

    Oyemade, Ura Jean, Ed.; Brandon-Monye, Deloris, Ed.

    Five plenary session presentations and summaries of 10 panel sessions held at a forum entitled "The Ecology of Substance Abuse: Toward Primary Prevention among High-Risk Youth" are provided in this document, which focuses on black youth at high risk for alcohol and drug problems. Experts describe a comprehensive ecological approach to addressing…

  11. 78 FR 40141 - Farm Credit Administration Board; Sunshine Act; Regular Meeting

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-07-03

    .... until such time as the Board concludes its business. FOR FURTHER INFORMATION CONTACT: Dale L. Aultman... the accessibility to Board meetings, persons requiring assistance ] should make arrangements...

  12. 77 FR 16810 - New England Fishery Management Council; Public Hearings

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-03-22

    ... National Oceanic and Atmospheric Administration RIN 0648-XB104 New England Fishery Management Council... to Paul J. Howard, Executive Director, New England Fishery Management Council, 50 Water Street, Mill.... Howard, Executive Director, New England Fishery Management Council; telephone: (978)...

  13. Mapping Me: Mapping Identity among Academically High-Performing Black Males

    ERIC Educational Resources Information Center

    Flennaugh, Terry K.

    2016-01-01

    Background: Scholars have argued that far too little research has examined the complex processes that many Black males undertake in constructing identities that function in schools (Howard & Flennaugh, 2011; Howard, Flennaugh, & Terry, 2012; Terry, Flennaugh, Blackmon, & Howard, 2014). Some have highlighted the perpetuation of a false…

  14. 75 FR 743 - Freedom of Information Act, Privacy Act of 1974; Implementation

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-01-06

    ... deleted from the Department's inventory of systems of records on April 17, 1992, at 57 FR 13900 had not...: Effective Date: January 6, 2010. FOR FURTHER INFORMATION CONTACT: Dale Underwood, Privacy Act Officer, Department of the Treasury, phone number 202-622-0874 or dale.underwood@do.treas.gov ....

  15. 14. UNIDENTIFIED CABLESTAYED SUSPENSION BRIDGE WITH TIMBER RAILING OF TEN ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    14. UNIDENTIFIED CABLE-STAYED SUSPENSION BRIDGE WITH TIMBER RAILING OF TEN PANELS AND STONE PIER, SHOWING ELEMENTS SIMILAR TO E.E. RUNYON'S SUSPENSION BRIDGE PATENTS, BUT ALTERNATE CABLE PATTERN. 3/4 VIEW FROM BELOW. - Bluff Dale Suspension Bridge, Spanning Paluxy River at County Route 149, Bluff Dale, Erath County, TX

  16. 76 FR 54415 - Proposed Flood Elevation Determinations

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-09-01

    ... contents in those buildings. Corrections In the proposed rule published at 74 FR 12807 in the March 25... Williams Creek (backwater effects from Dale Hollow Lake). DATES: Comments are to be submitted on or before... Williams Creek (backwater effects from Dale Hollow Lake). In this notice, FEMA is publishing a...

  17. The Readability of an Unreadable Text.

    ERIC Educational Resources Information Center

    Gordon, Robert M.

    1980-01-01

    The Dale-Chall Readability Formula and the Fry Readability Graph were used to analyze passages of Plato's "Parmenides," a notoriously difficult literary piece. The readability levels of the text ranged from fourth to eighth grade (Dale-Chall) and from sixth to tenth grade (Fry), indicating the limitations of the readability tests. (DF)

  18. The Effectiveness of the Smog Index in Determining the Reading Levels of Business and Distributive Education Texts.

    ERIC Educational Resources Information Center

    Schultheis, Robert A.; Anderson, Roberta

    1982-01-01

    McLaughlin's Smog Index was compared to the Dale-Chall formula for the determination of reading levels of 48 textbooks in business and distributive education. A Modified Smog Index proved a valid substitute for the Dale-Chall formula when used to evaluate the reading levels of business and distributive education narrative. (Author/CT)

  19. 76 FR 76449 - Post Office Closing

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-12-07

    ... Post Office Closing AGENCY: Postal Regulatory Commission. ACTION: Notice. SUMMARY: This document informs the public that an appeal of the closing of the Spring Dale, West Virginia post office has been... four petitions for review of the Postal Service's determination to close the Spring Dale post office...

  20. 37 CFR 102.34 - Specific exemptions.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    .../DEPT-14; (D) Attorneys and Agents Registered to Practice Before the Office—COMMERCE/PAT-TM-1; (E... OF COMMERCE ADMINISTRATION DISCLOSURE OF GOVERNMENT INFORMATION Privacy Act § 102.34 Specific... are within this exemption are: COMMERCE/PAT-TM-6, COMMERCE/PAT-TM-7, COMMERCE/PAT-TM-8,...

  1. 37 CFR 102.34 - Specific exemptions.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    .../DEPT-14; (D) Attorneys and Agents Registered to Practice Before the Office—COMMERCE/PAT-TM-1; (E... OF COMMERCE ADMINISTRATION DISCLOSURE OF GOVERNMENT INFORMATION Privacy Act § 102.34 Specific... are within this exemption are: COMMERCE/PAT-TM-6, COMMERCE/PAT-TM-7, COMMERCE/PAT-TM-8,...

  2. Planning the Beginning with the End in Mind: Evaluating Outreach and Enrollment Strategies, A Case Study. An Interview with Pat Redmond, Health Director, Philadelphia Citizens for Children and Youth, a NACA Member Organization. Issue Brief.

    ERIC Educational Resources Information Center

    Schmid, Margaret

    The State Children's Health Insurance Program (SCHIP) in 1997 signaled a major increase in time, energy, creativity, and money devoted to enrolling eligible children in health insurance programs. Child advocates and others have focused on developing outreach and enrollment strategies to bring the benefits of these new SCHIP programs to children…

  3. Multi-scale Functional and Molecular Photoacoustic Tomography

    PubMed Central

    Yao, Junjie; Xia, Jun; Wang, Lihong V.

    2015-01-01

    Photoacoustic tomography (PAT) combines rich optical absorption contrast with the high spatial resolution of ultrasound at depths in tissue. The high scalability of PAT has enabled anatomical imaging of biological structures ranging from organelles to organs. The inherent functional and molecular imaging capabilities of PAT have further allowed it to measure important physiological parameters and track critical cellular activities. Integration of PAT with other imaging technologies provides complementary capabilities and can potentially accelerate the clinical translation of PAT. PMID:25933617

  4. Problemes theoriques et methodologiques dans l'etude des langues/dialectes en contact aux niveaux macrologique et micrologique = Theoretical and Methodological Issues in the Study of Languages/Dialects in Contact at Macro- and Micro-Logical Levels of Analysis. Proceedings of the International Conference DALE (University of London)/ICRB (Laval University, Quebec)/ICSBT (Vrije Universiteit te Brussel) (London, England, May 23-26, 1985).

    ERIC Educational Resources Information Center

    Blanc, Michel, Ed.; Hamers, Josiane F., Ed.

    Papers from an international conference on the interaction of languages and dialects in contact are presented in this volume. Papers include: "Quelques reflexions sur la variation linguistique"; "The Investigation of 'Language Continuum' and 'Diglossia': A Macrological Case Study and Theoretical Model"; "A Survey of Dialect Grammar in British…

  5. Open Rotor: New Option for Jet Engines

    NASA Video Gallery

    NASA's Dale Van Zante describes how the open rotor propulsion system will be tested in a wind tunnel at NASA's Glenn Research Center. Open rotor aircraft engines use high-speed propellers and are c...

  6. 75 FR 77044 - Financial Management Service; Privacy Act of 1974, as Amended; System of Records

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-12-10

    ...'' (Document Number 2010-30297), published on December 3, 2010, at 75 FR 75546. The document will be revised... Management Service. DATES: December 10, 2010. FOR FURTHER INFORMATION CONTACT: Dale Underwood, Privacy...

  7. 2. VIEW SOUTHEAST, WEST FRONT OF BUILDINGS 25. 26; NORTH ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    2. VIEW SOUTHEAST, WEST FRONT OF BUILDINGS 25. 26; NORTH FRONT OF BUILDING 24: ROOF GABLE OF BUILDING 27 - U.S. Plant Introduction Station, Soil Conservation Service Cluster, 11601 Old Pond Road, Glenn Dale, Prince George's County, MD

  8. 1. VIEW EAST, WEST FRONT OF SOIL CONSERVATION SERVICE CLUSTER ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    1. VIEW EAST, WEST FRONT OF SOIL CONSERVATION SERVICE CLUSTER (BUILDINGS 24, 25, 26) - U.S. Plant Introduction Station, Soil Conservation Service Cluster, 11601 Old Pond Road, Glenn Dale, Prince George's County, MD

  9. 3. VIEW NORTHEAST, SOUTH FRONT OF SOIL CONSERVATION SERVICE CLUSTER ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    3. VIEW NORTHEAST, SOUTH FRONT OF SOIL CONSERVATION SERVICE CLUSTER (BUILDING 25) - U.S. Plant Introduction Station, Soil Conservation Service Cluster, 11601 Old Pond Road, Glenn Dale, Prince George's County, MD

  10. 76 FR 38653 - Ocean Transportation Intermediary License; Revocation

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-07-01

    ... Transgroup International. Address: 1400 Mittel Blvd., Suite A, Wood Dale, IL 60191. Date Revoked: May 29... to maintain a valid bond. License Number: 022540N. Name: Quality One International Shipping...

  11. All in the Family: The Sister Study

    MedlinePlus

    ... Study Chief, Epidemiology Branch The National Institute of Environmental Health Sciences (NIEHS) Photo courtesy of NIH/NIEHS By Dale ... being conducted by the NIH's National Institute of Environmental Health Sciences (NIEHS). The purpose of the Sister Study is ...

  12. 51. LAUNCH CREW IN FRONT OF BARGE ON RAIL BUGGIES. ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    51. LAUNCH CREW IN FRONT OF BARGE ON RAIL BUGGIES. (LEFT OT RIGHT: DALE WILBURN, GENE EVERLY, TIM DOEPELHEUER, JACK ROBERTS, JAMES REARICK, STEVE RAKUSKIN) - Hillman Barge & Construction Company, Paul Thomas Boulevard, Brownsville, Fayette County, PA

  13. 76 FR 82282 - Procurement List; Proposed Additions and Deletion

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-12-30

    ... Training Company, Caldwell, ID. Contracting Activity: Dept of the Navy, Navy Region Northwest Reserve.... MacArthur Blvd., Oklahoma City, OK. NPA: Dale Rogers Training Center, Inc., Oklahoma City,...

  14. 1. Historic American Buildings Survey Roger Sturtevant, Photographer Mar. 13, ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    1. Historic American Buildings Survey Roger Sturtevant, Photographer Mar. 13, 1934 RESIDENCE STREET (Ukiah St.) - Ukiah Street (Houses), Ukiah Street between Howard & Lansing, Mendocino, Mendocino County, CA

  15. Evaluating the 1995 BLS Projections.

    ERIC Educational Resources Information Center

    Rosenthal, Neal H.; Fullerton, Howard N., Jr.; Andreassen, Arthur; Veneri, Carolyn M.

    1997-01-01

    Includes "Introduction" (Neal H. Rosenthal); "Labor Force Projections" (Howard N. Fullerton, Jr.); "Industry Employment Projections" (Arthur Andreassen); and "Occupational Employment Projections" (Carolyn M. Veneri). (JOW)

  16. 7 CFR 1033.2 - Mideast marketing area.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ..., Delaware, Elkhart, Fayette, Fountain, Franklin, Fulton, Grant, Hamilton, Hancock, Hendricks, Henry, Howard..., Pulaski, Putnam, Randolph, Ripley, Rush, Shelby, St. Joseph, Starke, Steuben, Switzerland,...

  17. 7 CFR 1033.2 - Mideast marketing area.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ..., Delaware, Elkhart, Fayette, Fountain, Franklin, Fulton, Grant, Hamilton, Hancock, Hendricks, Henry, Howard..., Pulaski, Putnam, Randolph, Ripley, Rush, Shelby, St. Joseph, Starke, Steuben, Switzerland,...

  18. 76 FR 66718 - Clean Air Act Advisory Committee (CAAAC): Notice of Meeting

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-10-27

    ..., please contact Pat Childers, Office of Air and Radiation, U.S. EPA (202) 564-1082, FAX (202) 564-1352 or...-1082 or childers.pat@epa.gov . To request accommodation of a disability, please contact Mr....

  19. Cost and design study of modular small hydro plants. Volume 2. Appendixes. Final report

    SciTech Connect

    Pereira, L.

    1984-06-01

    The following appendices are included: major mechanical equipment and auxiliary electrical equipment for pumps as turbines (PAT), siphon penstock, prefabricated powerhouse, reliability of PAT installations, representative site investigation data, and case studies of four sites. (MHR)

  20. Tutorial on photoacoustic tomography

    NASA Astrophysics Data System (ADS)

    Zhou, Yong; Yao, Junjie; Wang, Lihong V.

    2016-06-01

    Photoacoustic tomography (PAT) has become one of the fastest growing fields in biomedical optics. Unlike pure optical imaging, such as confocal microscopy and two-photon microscopy, PAT employs acoustic detection to image optical absorption contrast with high-resolution deep into scattering tissue. So far, PAT has been widely used for multiscale anatomical, functional, and molecular imaging of biological tissues. We focus on PAT's basic principles, major implementations, imaging contrasts, and recent applications.

  1. Transgenic cry1C(⁎) gene rough rice line T1C-19 does not change the host preferences of the non-target stored product pest, Rhyzopertha dominica (Fabricius) (Coleoptera: Bostrichidae), and its parasitoid wasp, Anisopteromalus calandrae (Howard) (Hymenoptera: Pteromalidae).

    PubMed

    Sun, Xiao; Yan, Miao-Jun; Zhang, Aijun; Wang, Man-Qun

    2015-10-01

    Rough rice grains are often stored for extended periods before they are used or consumed. However, during storage, the rough rice is vulnerable to insect infestation, resulting in significant economic loss. Previous studies have shown that volatiles cues, physical characteristics, and taste chemicals on the grains could be the important key behavior factors for storage insect pests to locate the hosts and select oviposition sites. It is also well known that the transgenic Bt rough rice line T1C-19, which expresses a cry1C(⁎) gene has a high resistance to Lepidoptera pests. However, there were no evidences to show the consequences of host preference for non-target insect pests after growing Bt transgenic rice. In this study, the potential key factors of Bt rough rice were investigated for their impacts on the behaviors of non-target pest lesser grain borer Rhyzopertha dominica, the main weevil pest of grain and its parasitic wasps Anisopteromalus calandrae, the natural enemy of the beetle. Both electronic nose and electronic tongue analyses showed that the parameters of Bt rough rice were analogous to those of the non-Bt rough rice. The volatile profiles of Bt and non-Bt rough rice examined by gas chromatographic mass spectrometry (GC-MS) were similar. For most volatile compounds, there were no significantly quantitative differences in compound quantities between Bt and non-Bt rough rice. The densities of sclereids and trichomes on the rough rice husk surface were statistically equal in Bt and non-Bt rough rice. The non-target pest, R. dominica, and its parasitoid wasp, A. calandrae, were attracted to both rough rice and could not distinguish the transgenic T1C-19 from the isogenic rough rice. These results demonstrated that Bt rough rice has no negative impacts on the host preference behaviors of non-target stored product pest R. dominica and its parasitoid A. calandrae.

  2. 75 FR 71072 - Extension of the Patent Application Backlog Reduction Stimulus Plan

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-11-22

    ... Stimulus Plan, 74 FR 62285 (Nov. 27, 2009), 1349 Off. Gaz. Pat. Off. 304 (Dec. 22, 2009) (notice). The... Reduction Stimulus Plan, 74 FR at 62287, 1349 Off. Gaz. Pat. Off. at 306. The USPTO extended the plan for an... Plan, 75 FR 5041 (February 1, 2010), 1351 Off. Gaz. Pat. Off. 202 (February 23, 2010)....

  3. Construct and Predictive Validities of the Perceptual Ability Test.

    ERIC Educational Resources Information Center

    Kramer, Gene A.; And Others

    1989-01-01

    The construct and predictive validities of the Perceptual Ability Test (PAT) were examined. The results indicate that each of the subtests exhibits different predictive validity. A linear combination of PAT subtest scores was found to be more predictive of first-year dental school technique performance than the total PAT score. (Author/MLW)

  4. Partnership for a Drug-Free America: Partnership Attitude Tracking Study. Teens: Ethnic and Racial Trends, Spring 2002.

    ERIC Educational Resources Information Center

    Delaney, Barbara

    The annual Partnership Attitude Tracking Study (PATS) tracks consumers' attitudes about illegal drugs. PATS consists of two nationally projectable samples: a teen sample for students in grades 7-12 and a parent sample. The 2002 PATS, conducted in homes and schools, collected data using self-report surveys. Results indicate that after a decade of…

  5. Rickettsia typhi Possesses Phospholipase A2 Enzymes that Are Involved in Infection of Host Cells

    PubMed Central

    Rahman, M. Sayeedur; Gillespie, Joseph J.; Kaur, Simran Jeet; Sears, Khandra T.; Ceraul, Shane M.; Beier-Sexton, Magda; Azad, Abdu F.

    2013-01-01

    The long-standing proposal that phospholipase A2 (PLA2) enzymes are involved in rickettsial infection of host cells has been given support by the recent characterization of a patatin phospholipase (Pat2) with PLA2 activity from the pathogens Rickettsia prowazekii and R. typhi. However, pat2 is not encoded in all Rickettsia genomes; yet another uncharacterized patatin (Pat1) is indeed ubiquitous. Here, evolutionary analysis of both patatins across 46 Rickettsia genomes revealed 1) pat1 and pat2 loci are syntenic across all genomes, 2) both Pat1 and Pat2 do not contain predicted Sec-dependent signal sequences, 3) pat2 has been pseudogenized multiple times in rickettsial evolution, and 4) ubiquitous pat1 forms two divergent groups (pat1A and pat1B) with strong evidence for recombination between pat1B and plasmid-encoded homologs. In light of these findings, we extended the characterization of R. typhi Pat1 and Pat2 proteins and determined their role in the infection process. As previously demonstrated for Pat2, we determined that 1) Pat1 is expressed and secreted into the host cytoplasm during R. typhi infection, 2) expression of recombinant Pat1 is cytotoxic to yeast cells, 3) recombinant Pat1 possesses PLA2 activity that requires a host cofactor, and 4) both Pat1 cytotoxicity and PLA2 activity were reduced by PLA2 inhibitors and abolished by site-directed mutagenesis of catalytic Ser/Asp residues. To ascertain the role of Pat1 and Pat2 in R. typhi infection, antibodies to both proteins were used to pretreat rickettsiae. Subsequent invasion and plaque assays both indicated a significant decrease in R. typhi infection compared to that by pre-immune IgG. Furthermore, antibody-pretreatment of R. typhi blocked/delayed phagosomal escapes. Together, these data suggest both enzymes are involved early in the infection process. Collectively, our study suggests that R. typhi utilizes two evolutionary divergent patatin phospholipases to support its intracellular life cycle, a

  6. The Role of Home-Visiting Programs in Preventing Child Abuse and Neglect

    ERIC Educational Resources Information Center

    Howard, Kimberly S.; Brooks-Gunn, Jeanne

    2009-01-01

    Kimberly Howard and Jeanne Brooks-Gunn examine home visiting, an increasingly popular method for delivering services for families, as a strategy for preventing child abuse and neglect. They focus on early interventions because infants are at greater risk for child abuse and neglect than are older children. In their article, Howard and Brooks-Gunn…

  7. A World without Walls. Dimension: Languages 71. Proceedings of the Southern Conference on Language Teaching (7th, Atlanta, Georgia, October 1971).

    ERIC Educational Resources Information Center

    Wilkins, George W., Jr., Ed.

    Presentations on aspects of foreign language instruction include: "A World Without Walls" (Lorraine A. Strasheim); "Practical Aspects of Individualizing Foreign Language Instruction" (Howard B. Altman); "Teaching Foreign Language Through Literature" (Jeanne Varney Pleasants); "Individualization of Foreign Language Instruction" (Howard B. Altman);…

  8. 77 FR 56138 - World Trade Center Health Program; Addition of Certain Types of Cancer to the List of WTC-Related...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-09-12

    ... pleura; and other ill-defined sites in the respiratory system and intrathoracic organs Mesothelioma... proposed rulemaking (77 FR 35574) proposing to add certain cancers to the List of WTC-Related Health... published on June 13, 2012 (77 FR 35574). \\5\\ Howard J . October 5, 2011 Letter from John Howard,...

  9. The Art of Math

    ERIC Educational Resources Information Center

    Kim, Una; Stabley, Angela

    2010-01-01

    In the movie "A Beautiful Mind" there is a scene where future Nobel prize winner John Nash is captivated by a fellow student's tie. When asked about his behavior Nash quips, "There has to be a mathematical explanation for how bad that tie is." (Grazer & Howard, 2000) In this light moment, director Ron Howard attempts to show us that the mind of a…

  10. 76 FR 9358 - Notice of Proposed Withdrawal Extension and Opportunity for Public Meeting; Montana

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-02-17

    ... Management extend PLO No. 6881 (56 FR 47414 (1991)), which withdrew approximately 95 acres of National Forest... Federal funds at the Howard Lake, Ross Creek, and Yaak Falls Recreation Areas. The withdrawal created by... investment of Federal funds at the Howard Lake, Ross Creek and Yaak Falls Recreation Areas. The use of...

  11. 76 FR 61738 - Public Land Order No. 7781; Extension of Public Land Order No. 6881; Montana

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-10-05

    ...: Public Land Order No. 6881 (56 FR 47414 (1991)) which withdrew 95 acres of National Forest System lands... extension is necessary to continue the protection of the United States Forest Service's Howard Lake, Ross... the investment of Federal funds at the Howard Lake, Ross Creek, and Yaak Falls Recreation Areas....

  12. Economic and Workforce Development. [Final Task Force Report].

    ERIC Educational Resources Information Center

    Howard Community Coll., Columbia, MD.

    This document describes the vision and priorities of Howard Community College's (HCC's) Economic and Workforce Development Task Force. The task force's commission was to identify the long- and short-term skills businesses will demand of the workforce in the Greater Baltimore area and what Howard Community College must do to continue developing…

  13. Census Analysis: A Look at HCC Credit Students Based on the 1980 Census. Research Report Number 30.

    ERIC Educational Resources Information Center

    Nespoli, Lawrence A.; Radcliffe, Susan K.

    A study was conducted by Howard Community College (HCC) to identify the geographic make-up of HCC credit students and to examine importance factors influencing college attendance. Using census software, data on 93.4% of HCC's 1980 credit students (N=4,013) were compared with 1980 census data on Howard County to determine participation rates for…

  14. Travel and Correspondence

    ERIC Educational Resources Information Center

    Shauck, Barry

    2004-01-01

    Professional development through the advanced planning of themed exhibitions provides art instructors in Howard County the impetus for many art problems. This art problem, centered on the art and writing contained in the storybooks of Holly Hobbie. In late summer, the art teaching staff of the Howard County Public School System gathered at the…

  15. Aussie Battler, or Worldly Opportunist?

    ERIC Educational Resources Information Center

    Walter, James

    2004-01-01

    In this article, the author comments on Judith Brett's article (see EJ848172). Brett dissects the differences between the Howard world-view and that of the intelligentsia, using Robert Merton's dichotomy between "locals" and "cosmopolitans" as her means. On this account, Howard's preoccupation with national sovereignty--and hence with border…

  16. Changing Views of Culture and Behavior: An Interview with Robert Serpell

    ERIC Educational Resources Information Center

    Lockett, Charles T.

    2003-01-01

    Charles Lockett is an Assistant Professor in the School of Psychology at James Madison University, where he teaches developmental psychology as well as advanced topic courses in cultural psychology and the psychology of race and racism. A graduate of Howard University, Lockett credits Howard's Preparing Future Faculty Fellowship Program for his…

  17. What Helps Us Learn?

    ERIC Educational Resources Information Center

    Educational Leadership, 2010

    2010-01-01

    This article presents comments of high school students at the Howard Gardner School in Alexandria, Virginia, who were asked, What should teachers know about students to help them learn? Twelve high school students from the Howard Gardner School in Alexandria, Virginia, describe how their best teachers get to know them and thus were more able to…

  18. A Practical Guide to Photoacoustic Tomography in the Life Sciences

    PubMed Central

    Wang, Lihong V.; Yao, Junjie

    2016-01-01

    The life sciences can benefit greatly from imaging technologies that connect microscopic discoveries with macroscopic observations. Photoacoustic tomography (PAT), a highly sensitive modality for imaging rich optical absorption contrast over a wide range of spatial scales at high speed, is uniquely positioned for this need. In PAT, endogenous contrast reveals tissue’s anatomical, functional, metabolic, and histologic properties, and exogenous contrast provides molecular and cellular specificity. The spatial scale of PAT covers organelles, cells, tissues, organs, and small-animal organisms. Consequently, PAT is complementary to other imaging modalities in contrast mechanism, penetration, spatial resolution, and temporal resolution. We review the fundamentals of PAT and provide practical guidelines to the broad life science community for matching PAT systems with research needs. We also summarize the most promising biomedical applications of PAT, discuss related challenges, and envision its potential to lead to further breakthroughs. PMID:27467726

  19. Expression of phosphinothricin N-acetyltransferase in Escherichia coli and Pseudomonas fluorescens: influence of mRNA secondary structure, host, and other physiological conditions.

    PubMed

    Madduri, Krishna M; Snodderley, Erika M

    2007-10-01

    Expression of a plant codon optimized pat gene encoding phosphinothricin acetyltransferase (PAT) in bacterial expression systems required modification of the 5' end of the pat ORF. Modifications necessary for improving the expression were identified by a coupled in vitro transcription and translation process. The dramatic improvement in the expression of PAT was due to the removal of a potential secondary structure that could have resulted in the inhibition of translational initiation. Therefore, in vitro transcription and translation is a versatile tool to optimize gene sequence for protein overexpression. Additionally, this method was shown to be successful in both Escherichia coli and Pseudomonas fluorescens. Gene sequence optimization and choice of host along with cultivation conditions also had major impact on PAT expression. P. fluorescens was a better host than E. coli resulting in 30-fold more expression of PAT. We were able to recover approximately 95mg of purified PAT from P. fluorescens using a three step chromatographic process.

  20. A Golgi and tonoplast localized S-acyl transferase is involved in cell expansion, cell division, vascular patterning and fertility in Arabidopsis

    PubMed Central

    Qi, Baoxiu; Doughty, James; Hooley, Richard

    2013-01-01

    S-acylation of eukaryotic proteins is the reversible attachment of palmitic or stearic acid to cysteine residues, catalysed by protein S-acyl transferases that share an Asp-His-His-Cys (DHHC) motif. Previous evidence suggests that in Arabidopsis S-acylation is involved in the control of cell size, polarity and the growth of pollen tubes and root hairs. Using a combination of yeast genetics, biochemistry, cell biology and loss of function genetics the roles of a member of the protein S-acyl transferase PAT family, AtPAT10 (At3g51390), have been explored. In keeping with its role as a PAT, AtPAT10 auto-S-acylates, and partially complements the yeast akr1 PAT mutant, and this requires Cys192 of the DHHC motif. In Arabidopsis AtPAT10 is localized in the Golgi stack, trans-Golgi network/early endosome and tonoplast. Loss-of-function mutants have a pleiotropic phenotype involving cell expansion and division, vascular patterning, and fertility that is rescued by wild-type AtPAT10 but not by catalytically inactive AtPAT10C192A. This supports the hypothesis that AtPAT10 is functionally independent of the other Arabidopsis PATs. Our findings demonstrate a growing importance of protein S-acylation in plants, and reveal a Golgi and tonoplast located S-acylation mechanism that affects a range of events during growth and development in Arabidopsis. PMID:23795888

  1. A near-infrared fluorescence assay method to detect patulin in food.

    PubMed

    Pennacchio, Anna; Varriale, Antonio; Esposito, Maria Grazia; Staiano, Maria; D'Auria, Sabato

    2015-07-15

    Patulin (PAT) is a toxic secondary metabolite (mycotoxin) of different fungal species belonging to the genera Penicillium, Aspergillus, and Byssochlamys. They can grow on a large variety of food, including fruits, grains, and cheese. The amount of PAT in apple derivative products is a crucial issue because it is the measure of the quality of both the used raw products and the performed production process. Actually, all current methodologies used for the quantification of PAT are time-consuming and require skilled personnel beyond the sample pretreatment methods (e.g., high-performance liquid chromatography, mass spectrometry, and electrophoresis techniques). In this work, we present a novel fluorescence polarization approach based on the use of emergent near-infrared (NIR) fluorescence probes. The use of these fluorophores coupled to anti-PAT antibodies makes possible the detection of PAT directly in apple juice without any sample pretreatment. This methodology is based on the increase of fluorescence polarization emission of a fluorescence-labeled PAT derivative on binding to specific antibodies. A competition between PAT and the fluorescence-labeled PAT derivative allowed detecting PAT. The limit of detection of the method is 0.06 μg/L, a value that is lower than maximum residue limit of PAT fixed at 50 μg/L from European Union regulation.

  2. Precocious leaf senescence by functional loss of PROTEIN S-ACYL TRANSFERASE14 involves the NPR1-dependent salicylic acid signaling

    PubMed Central

    Zhao, Xin-Ying; Wang, Jia-Gang; Song, Shi-Jian; Wang, Qun; Kang, Hui; Zhang, Yan; Li, Sha

    2016-01-01

    We report here that Arabidopsis PROTEIN S-ACYL TRANSFERASE14 (PAT14), through its palmitate transferase activity, acts at the vacuolar trafficking route to repress salicylic acid (SA) signaling, thus mediating age-dependent but not carbon starvation-induced leaf senescence. Functional loss of PAT14 resulted in precocious leaf senescence and its transcriptomic analysis revealed that senescence was dependent on salicylic acid. Overexpressing PAT14 suppressed the expression of SA responsive genes. Introducing the SA deficient mutants, npr1-5 and NahG, but not other hormonal mutants, completely suppressed the precocious leaf senescence of PAT14 loss-of-function, further supporting the epistatic relation between PAT14 and the SA pathway. By confocal fluorescence microscopy, we showed that PAT14 is localized at the Golgi, the trans-Golg network/early endosome, and prevacuolar compartments, indicating its roles through vacuolar trafficking. By reporter analysis and real time PCRs, we showed that the expression PAT14, unlike most of the senescence associated genes, is not developmentally regulated, suggesting post-transcriptional regulatory mechanisms on its functionality. We further showed that the maize and wheat homologs of PAT14 fully rescued the precocious leaf senescence of pat14-2, demonstrating that the role of PAT14 in suppressing SA signaling during age-dependent leaf senescence is evolutionarily conserved between dicots and monocots. PMID:26842807

  3. metal ion interactions of picoline-2-aldehyde thiosemicarbazone.

    PubMed

    Leggett, D J; McBryde, W A

    The reactions of picoline-2-aldehyde thiosemicarbazone (PATS) with silver, mercury, iron(II) and cobalt have been investigated in various environments. The compositions of the complexes have been investigated by continuous variation and molar ratio methods. Stability constants have been evaluated by means of SCOGS and a new program SQUAD. The formation constants, measured at 25 degrees and 0.10M ionic strength were as follows: Ag(PATS), logbeta(101) = 13.40; HgH(PATS), log beta(1110) = 23.6; HgH(2)(PATS)(2), log beta(1220) = 42.1; HgH(2)(PATS)(EDTA), log beta = 44.0; FeH(3)(PATS)(3), log beta(133) = 44.9; FeH(2)(PATS)(3), log beta(123) = 41.7; FeH(PATS)(3), log beta(113) = 38.4; Fe(PATS)(3), log beta(103) = 34.2. A tentative value for a cobalt complex is also suggested. A computer program, suitable for calculation of optimum conditions for a chemical analysis is also introduced and its use is illustrated for the silver-PATS-EDTA system.

  4. A Golgi and tonoplast localized S-acyl transferase is involved in cell expansion, cell division, vascular patterning and fertility in Arabidopsis.

    PubMed

    Qi, Baoxiu; Doughty, James; Hooley, Richard

    2013-10-01

    S-acylation of eukaryotic proteins is the reversible attachment of palmitic or stearic acid to cysteine residues, catalysed by protein S-acyl transferases that share an Asp-His-His-Cys (DHHC) motif. Previous evidence suggests that in Arabidopsis S-acylation is involved in the control of cell size, polarity and the growth of pollen tubes and root hairs. Using a combination of yeast genetics, biochemistry, cell biology and loss of function genetics the roles of a member of the protein S-acyl transferase PAT family, AtPAT10 (At3g51390), have been explored. In keeping with its role as a PAT, AtPAT10 auto-S-acylates, and partially complements the yeast akr1 PAT mutant, and this requires Cys(192) of the DHHC motif. In Arabidopsis AtPAT10 is localized in the Golgi stack, trans-Golgi network/early endosome and tonoplast. Loss-of-function mutants have a pleiotropic phenotype involving cell expansion and division, vascular patterning, and fertility that is rescued by wild-type AtPAT10 but not by catalytically inactive AtPAT10C(192) A. This supports the hypothesis that AtPAT10 is functionally independent of the other Arabidopsis PATs. Our findings demonstrate a growing importance of protein S-acylation in plants, and reveal a Golgi and tonoplast located S-acylation mechanism that affects a range of events during growth and development in Arabidopsis. PMID:23795888

  5. Patulin is a cultivar-dependent aggressiveness factor favouring the colonization of apples by Penicillium expansum.

    PubMed

    Snini, Selma P; Tannous, Joanna; Heuillard, Pauline; Bailly, Sylviane; Lippi, Yannick; Zehraoui, Enric; Barreau, Christian; Oswald, Isabelle P; Puel, Olivier

    2016-08-01

    The blue mould decay of apples is caused by Penicillium expansum and is associated with contamination by patulin, a worldwide regulated mycotoxin. Recently, a cluster of 15 genes (patA-patO) involved in patulin biosynthesis was identified in P. expansum. blast analysis revealed that patL encodes a Cys6 zinc finger regulatory factor. The deletion of patL caused a drastic decrease in the expression of all pat genes, leading to an absence of patulin production. Pathogenicity studies performed on 13 apple varieties indicated that the PeΔpatL strain could still infect apples, but the intensity of symptoms was weaker compared with the wild-type strain. A lower growth rate was observed in the PeΔpatL strain when this strain was grown on nine of the 13 apple varieties tested. In the complemented PeΔpatL:patL strain, the ability to grow normally in apple and the production of patulin were restored. Our results clearly demonstrate that patulin is not indispensable in the initiation of the disease, but acts as a cultivar-dependent aggressiveness factor for P. expansum. This conclusion was strengthened by the fact that the addition of patulin to apple infected by the PeΔpatL mutant restored the normal fungal colonization in apple. PMID:26582186

  6. Relationships between the ABC-Exporter HetC and Peptides that Regulate the Spatiotemporal Pattern of Heterocyst Distribution in Anabaena

    PubMed Central

    Corrales-Guerrero, Laura; Flores, Enrique; Herrero, Antonia

    2014-01-01

    In the model cyanobacterium Anabaena sp. PCC 7120, cells called heterocysts that are specialized in the fixation of atmospheric nitrogen differentiate from vegetative cells of the filament in the absence of combined nitrogen. Heterocysts follow a specific distribution pattern along the filament, and a number of regulators have been identified that influence the heterocyst pattern. PatS and HetN, expressed in the differentiating cells, inhibit the differentiation of neighboring cells. At least PatS appears to be processed and transferred from cell to cell. HetC is similar to ABC exporters and is required for differentiation. We present an epistasis analysis of these regulatory genes and of genes, hetP and asr2819, successively downstream from hetC, and we have studied the localization of HetC and HetP by use of GFP fusions. Inactivation of patS, but not of hetN, allowed differentiation to proceed in a hetC background, whereas inactivation of hetC in patS or patS hetN backgrounds decreased the frequency of contiguous proheterocysts. A HetC-GFP protein is localized to the heterocysts and especially near their cell poles, and a putative HetC peptidase domain was required for heterocyst differentiation but not for HetC-GFP localization. hetP is also required for heterocyst differentiation. A HetP-GFP protein localized mostly near the heterocyst poles. ORF asr2819, which we denote patC, encodes an 84-residue peptide and is induced upon nitrogen step-down. Inactivation of patC led to a late spreading of the heterocyst pattern. Whereas HetC and HetP appear to have linked functions that allow heterocyst differentiation to progress, PatC may have a role in selecting sites of differentiation, suggesting that these closely positioned genes may be functionally related. PMID:25121608

  7. Relationships between the ABC-exporter HetC and peptides that regulate the spatiotemporal pattern of heterocyst distribution in Anabaena.

    PubMed

    Corrales-Guerrero, Laura; Flores, Enrique; Herrero, Antonia

    2014-01-01

    In the model cyanobacterium Anabaena sp. PCC 7120, cells called heterocysts that are specialized in the fixation of atmospheric nitrogen differentiate from vegetative cells of the filament in the absence of combined nitrogen. Heterocysts follow a specific distribution pattern along the filament, and a number of regulators have been identified that influence the heterocyst pattern. PatS and HetN, expressed in the differentiating cells, inhibit the differentiation of neighboring cells. At least PatS appears to be processed and transferred from cell to cell. HetC is similar to ABC exporters and is required for differentiation. We present an epistasis analysis of these regulatory genes and of genes, hetP and asr2819, successively downstream from hetC, and we have studied the localization of HetC and HetP by use of GFP fusions. Inactivation of patS, but not of hetN, allowed differentiation to proceed in a hetC background, whereas inactivation of hetC in patS or patS hetN backgrounds decreased the frequency of contiguous proheterocysts. A HetC-GFP protein is localized to the heterocysts and especially near their cell poles, and a putative HetC peptidase domain was required for heterocyst differentiation but not for HetC-GFP localization. hetP is also required for heterocyst differentiation. A HetP-GFP protein localized mostly near the heterocyst poles. ORF asr2819, which we denote patC, encodes an 84-residue peptide and is induced upon nitrogen step-down. Inactivation of patC led to a late spreading of the heterocyst pattern. Whereas HetC and HetP appear to have linked functions that allow heterocyst differentiation to progress, PatC may have a role in selecting sites of differentiation, suggesting that these closely positioned genes may be functionally related.

  8. Relationships between the ABC-exporter HetC and peptides that regulate the spatiotemporal pattern of heterocyst distribution in Anabaena.

    PubMed

    Corrales-Guerrero, Laura; Flores, Enrique; Herrero, Antonia

    2014-01-01

    In the model cyanobacterium Anabaena sp. PCC 7120, cells called heterocysts that are specialized in the fixation of atmospheric nitrogen differentiate from vegetative cells of the filament in the absence of combined nitrogen. Heterocysts follow a specific distribution pattern along the filament, and a number of regulators have been identified that influence the heterocyst pattern. PatS and HetN, expressed in the differentiating cells, inhibit the differentiation of neighboring cells. At least PatS appears to be processed and transferred from cell to cell. HetC is similar to ABC exporters and is required for differentiation. We present an epistasis analysis of these regulatory genes and of genes, hetP and asr2819, successively downstream from hetC, and we have studied the localization of HetC and HetP by use of GFP fusions. Inactivation of patS, but not of hetN, allowed differentiation to proceed in a hetC background, whereas inactivation of hetC in patS or patS hetN backgrounds decreased the frequency of contiguous proheterocysts. A HetC-GFP protein is localized to the heterocysts and especially near their cell poles, and a putative HetC peptidase domain was required for heterocyst differentiation but not for HetC-GFP localization. hetP is also required for heterocyst differentiation. A HetP-GFP protein localized mostly near the heterocyst poles. ORF asr2819, which we denote patC, encodes an 84-residue peptide and is induced upon nitrogen step-down. Inactivation of patC led to a late spreading of the heterocyst pattern. Whereas HetC and HetP appear to have linked functions that allow heterocyst differentiation to progress, PatC may have a role in selecting sites of differentiation, suggesting that these closely positioned genes may be functionally related. PMID:25121608

  9. Delivery of paclitaxel from cobalt–chromium alloy surfaces without polymeric carriers

    PubMed Central

    Mani, Gopinath; Macias, Celia E.; Feldman, Marc D.; Marton, Denes; Oh, Sunho; Agrawal, C. Mauli

    2014-01-01

    Polymer-based carriers are commonly used to deliver drugs from stents. However, adverse responses to polymer coatings have raised serious concerns. This research is focused on delivering drugs from stents without using polymers or any carriers. Paclitaxel (PAT), an anti-restenotic drug, has strong adhesion towards a variety of material surfaces. In this study, we have utilized such natural adhesion property of PAT to attach these molecules directly to cobalt–chromium (Co–Cr) alloy, an ultra-thin stent strut material. Four different groups of drug coated specimens were prepared by directly adding PAT to Co–Cr alloy surfaces: Group-A (PAT coated, unheated, and ethanol cleaned); Group-B (PAT coated, heat treated, and ethanol cleaned); Group-C (PAT coated, unheated, and not ethanol cleaned); and Group-D (PAT coated, heat treated and not ethanol cleaned). In vitro drug release of these specimens was investigated using high performance liquid chromatography. Groups A and B showed sustained PAT release for up to 56 days. A simple ethanol cleaning procedure after PAT deposition can remove the loosely bound drug crystals from the alloy surfaces and thereby allowing the remaining strongly bound drug molecules to be released at a sustained rate. The heat treatment after PAT coating further improved the stability of PAT on Co–Cr alloy and allowed the drug to be delivered at a much slower rate, especially during the initial 7 days. The specimens which were not cleaned in ethanol, Groups C and D, showed burst release. PAT coated Co–Cr alloy specimens were thoroughly characterized using scanning electron microscopy, atomic force microscopy, and X-ray photoelectron spectroscopy. These techniques were collectively useful in studying the morphology, distribution, and attachment of PAT molecules on Co–Cr alloy surfaces. Thus, this study suggests the potential for delivering paclitaxel from Co–Cr alloy surfaces without using any carriers. PMID:20398928

  10. Vancomycın resıstant enterococcus bacteremıa ın a patıent wıth Pneumocystis jiroveci pneumonıa, granulocystıc sarcoma and acute respıratory dıstress syndrome

    PubMed Central

    Emre, Julide Celdir; Baysak, Aysegul; Oz, Adnan Tolga; Ece, Gulfem; Arda, Bilgin; Bacakoglu, Feza

    2014-01-01

    In this case report we aimed to present a patient with granulocytic sarcomaa, neutropenic fever, ARDS and Pneumocystis jirovecii pneumoniae that was hospitalized in our intensive care unit. The patient recovered and then developed vancomycin resistant enterococci (VRE) bacteremia due to port catheter during follow up. The patient had risk factors for VRE bacteremia and he was administered linezolide without removing the catheter. He was discharged with recovery. PMID:25018799

  11. Contrast-enhanced photoacoustic tomography of human joints

    NASA Astrophysics Data System (ADS)

    Tian, Chao; Keswani, Rahul K.; Gandikota, Girish; Rosania, Gus R.; Wang, Xueding

    2016-03-01

    Photoacoustic tomography (PAT) provides a unique tool to diagnose inflammatory arthritis. However, the specificity and sensitivity of PAT based on endogenous contrasts is limited. The development of contrast enhanced PAT imaging modalities in combination with small molecule contrast agents could lead to improvements in diagnosis and treatment of joint disease. Accordingly, we adapted and tested a PAT clinical imaging system for imaging the human joints, in combination with a novel PAT contrast agent derived from an FDA-approved small molecule drug. Imaging results based on a photoacoustic and ultrasound (PA/US) dual-modality system revealed that this contrast-enhanced PAT imaging system may offer additional information beyond single-modality PA or US imaging system, for the imaging, diagnosis and assessment of inflammatory arthritis.

  12. 4-D Photoacoustic Tomography

    NASA Astrophysics Data System (ADS)

    Xiang, Liangzhong; Wang, Bo; Ji, Lijun; Jiang, Huabei

    2013-01-01

    Photoacoustic tomography (PAT) offers three-dimensional (3D) structural and functional imaging of living biological tissue with label-free, optical absorption contrast. These attributes lend PAT imaging to a wide variety of applications in clinical medicine and preclinical research. Despite advances in live animal imaging with PAT, there is still a need for 3D imaging at centimeter depths in real-time. We report the development of four dimensional (4D) PAT, which integrates time resolutions with 3D spatial resolution, obtained using spherical arrays of ultrasonic detectors. The 4D PAT technique generates motion pictures of imaged tissue, enabling real time tracking of dynamic physiological and pathological processes at hundred micrometer-millisecond resolutions. The 4D PAT technique is used here to image needle-based drug delivery and pharmacokinetics. We also use this technique to monitor 1) fast hemodynamic changes during inter-ictal epileptic seizures and 2) temperature variations during tumor thermal therapy.

  13. Geochemical survey of the Devil's Den Roadless Area, Rutland and Windsor counties, Vermont

    USGS Publications Warehouse

    Slack, J.F.; Atelsek, P.J.; Grosz, A.E.

    1985-01-01

    The Devils Den area is named for a large undercut cliff (Dale, 1915, p. 21) developed in Precambrian basement rocks. This undercut cliff forms a broad natural cave immediately west of and below Forest Service Road 10, at the head of Mt. Tabor Brook. Another much smaller cave is present in dolomite of probable Paleozoic (Early Cambrian) age on the east side of the same road. This smaller cave apparently is of artificial origin, having been made during early mining of the dolomite (Dale, 1915, p. 21). This man-made cave is the only evidence of previous mining activity within the study area.

  14. Are teachers of children and young adolescents responsive to suicide prevention training modules? Yes.

    PubMed

    Davidson, M W; Range, L M

    1999-01-01

    Both before and after a 1-hour suicide prevention training module, 75 elementary teachers-in-training read a 4-sentence vignette about a suicidal student ("Pat"), then completed 8 questions about their responses. Compared with pretraining, at post-training these teachers were more likely to say that they would send or escort Pat to the counselor's office, use written or verbal no-suicide agreements, call Pat's parents, believe Pat to be serious rather than simply seeking attention, and feel comfortable handling a similar situation. Increased proactive attitudes after one hour of training imply that teachers would benefit from periodic suicide awareness and prevention training modules.

  15. Parthenocarpic fruit development in tomato.

    PubMed

    Gorguet, B; van Heusden, A W; Lindhout, P

    2005-03-01

    Parthenocarpic fruit development is a very attractive trait for growers and consumers. In tomato, three main sources of facultative parthenocarpy, pat, pat-2, pat-3/pat-4, are known to have potential applications in agriculture. The parthenocarpic fruit development in these lines is triggered by a deregulation of the hormonal balance in some specific tissues. Auxins and gibberellins are considered as the key elements in parthenocarpic fruit development of those lines. An increased level of these hormones in the ovary can substitute for pollination and trigger fruit development. This has opened up genetic engineering approaches for parthenocarpy that have given promising results, both in quality and quantity of seedless fruit production. PMID:15822008

  16. Cold-Sensitive Pseudomonas RNA Polymerase II. Cold-Promoted Restriction of Bacteriophage CB3 and the Lack of Host-Dependent Bacteriophage-Specific RNA Transcription

    PubMed Central

    Sobieski, Rodney J.; Olsen, Ronald H.

    1973-01-01

    Cold-sensitive restriction of Pseudomonas phage CB3 by Pseudomonas aeruginosa strain PAT2 involves some aspect of CB3 specific RNA synthesis at 20 C. Experiments using chloramphenicol treatment and RNA-DNA hybridization establish that the amount of CB3 RNA present at 20 C is consistent with the known percentage of phage yielder cells at 20 C. Thus, it appears that nonyielder cells of PAT2 synthesize little or no phage-specific mRNA. Burgess technique extracted PAT2 RNA polymerase (RNAP) is cold sensitive when assayed in vitro with CB3 DNA at 20 C. However, it is not cold sensitive when either calf thymus or PAT2 DNA are the templates for transcription. Low ionic strength assay conditions eliminate the cold sensitivity of PAT2 RNAP. The effect of low ionic environments on transcription initiation along with the in vivo and in vitro suppression of cold sensitivity by host rifampin resistance suggests that the inability of CB3 to reproduce in PAT2 at 20 C is a cold-sensitive step in host RNAP initiation. Our modified RNAP extraction procedure for PAT2 and PAO1C also results in the recovery of cold-sensitive PAT2 RNAP with respect to CB3 DNA templates and points to basic enzymological differences between the two hosts. A model is presented for the unusual influence of temperature on the initiation process of both PAT2 and PAO1C on RNAP transcription. PMID:4202618

  17. A Photoacoustic Imaging System with Optimized Real-Time Parallel Reconstruction

    NASA Astrophysics Data System (ADS)

    Feng, Ting; Yuan, Jie; Yu, Yao; Zhou, Yu; Xu, Guan

    2013-10-01

    Biomedical photoacoustic tomography (PAT) provides anatomical, functional, metabolic, molecular, and genetic contrasts of vasculature, hemodynamics, oxygen metabolism, biomarkers, and gene expression. These attributes bring PAT to a wide variety of applications in clinical medicine and preclinical research. We report the development of a real-time PAT imaging system, which integrates signal scanning, image reconstruction and displaying photoacoustic images in real time. An optimized back projection algorithm for PAT imaging is proposed and tested on a latest graphics process unit based card. The whole system is built and tested in an experiment for monitoring moving blood events to validate the real-time performance of this system to image moving events.

  18. Rapid and sensitive determination of tryptophan, serotonin and psychoactive tryptamines by thin-layer chromatography/fluorescence detection.

    PubMed

    Kato, Noriyuki; Kojima, Takashi; Yoshiyagawa, Shinji; Ohta, Hikoto; Toriba, Akira; Nishimura, Hideo; Hayakawa, Kazuichi

    2007-03-23

    A rapid, sensitive and selective method for the determination of tryptophan (Trp), serotonin (5-HT) and psychoactive tryptamines (PATs) by thin-layer chromatography (TLC) with fluorescence detection is proposed. These compounds form fluorophores on the developing plate by heating after spraying with sodium hypochlorite, hydrogen peroxide or potassium hexacyanoferrate(III)-sodium hydroxide reagent. Fluorescent spots (vivid blue) were observed by irradiation with ultraviolet light (365 nm). The detection limits of Trp, 5-HT and PATs were in the range from 0.01 microg to 0.06 microg. This method was effectively applied to the detection of confiscated PAT powder and PAT in abusers' urine samples.

  19. ISS Update: Keeping the Flight Control Rooms Running

    NASA Video Gallery

    ISS Update Commentator Pat Ryan interviews Angela Bauer, Facilities Operations and Maintenance Group lead in the Mission Operations Directorate at Johnson Space Center. They discuss her group's rol...

  20. ISS Update: ATV-3’s Science Payload

    NASA Video Gallery

    ISS Update Commentator Pat Ryan interviews Camille Alleyne, Assistant Program Scientist for International Space Station. They discuss the science payload that was delivered to the station and crew ...