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Sample records for howard pat dale

  1. Dale Evarts

    EPA Pesticide Factsheets

    Dale leads the Climate, International, and Multimedia Group in the Office of Air Quality Planning and Standards (OAQPS) at the US EPA. His group is also leading efforts to address and exploit the linkages between climate change and air quality.

  2. John Galen Howard.

    ERIC Educational Resources Information Center

    Draper, Joan

    1979-01-01

    A biographical sketch of John Galen Howard, founder of the Department of Architecture at the University of California at Berkeley, is presented. Howard's conservative outlook and idealistic nature are examined and his influence on the curriculum at the university is traced. (PHR)

  3. Reinventing Howard's Law School.

    ERIC Educational Resources Information Center

    Glenn, Gwendolyn

    2001-01-01

    Describes efforts by Alice Gresham Bullock, dean of the Howard University School of Law, to improve the school (including building a new library and improving relations with faculty) and restore Howard to its previous stature as a leader in civil rights law. (EV)

  4. Who was John Howard?

    PubMed

    Lasure, E A

    1999-08-01

    John Howard was an 18th-century English philanthropist who made significant contributions in prison reform. Despite personal tragedy and an oppositional social climate, he became an early promoter of humane treatment for prisoners. Other reformers followed John Howard, making valuable contributions, but many challenges remain in the management of forensic hospitals and prison systems. Howard's legacy is not only the modernization of prison structures and programs, but also the work of numerous worldwide societies and associations that provide services for communities and prisoners.

  5. The Howard Zinn Show

    ERIC Educational Resources Information Center

    Sewall, Gilbert T.

    2012-01-01

    Howard Zinn's "A People's History of the United States" is the nation's best-known work of American history. It is also the nation's best-selling survey of American history, having sold two million copies since its publication in 1980 and still selling about 125,000 paperback copies yearly. The fifth and current edition covers America up…

  6. Astronaut Dale Gardner rehearses during EVA practice

    NASA Technical Reports Server (NTRS)

    1984-01-01

    Astronaut Dale A. Gardner, 51-A mission specialist, rehearses control of manned maneuvering unit (MMU) during a practice for an extravehicular activity (EVA). Gardner is in the Shuttle mockup and integration laboratory at JSC. Gardner works to deploy a large stinger device designed for locking onto the orbiting satellites via entering a spent engine's nozzle.

  7. Astronaut Dale Gardner rehearses during EVA practice

    NASA Technical Reports Server (NTRS)

    1984-01-01

    Astronaut Dale A. Gardner, 51-A mission specialist, rehearses control of manned maneuvering unit (MMU) during a practice for an extravehicular activity (EVA). Gardner is in the Shuttle mockup and integration laboratory at JSC. Gardner handles a stinger device to make initial contact with one of the two satellites they will be working with.

  8. Howard Zinn on Democratic Education

    ERIC Educational Resources Information Center

    Zinn, Howard

    2004-01-01

    Perhaps no other historian has had a more profound and revolutionary impact on American education than Howard Zinn. This is the first book devoted to his views on education and its role in a democratic society. "Howard Zinn on Democratic Education" describes what is missing from school textbooks and in classrooms--and how we move beyond these…

  9. Implementing PAT with Standards

    NASA Astrophysics Data System (ADS)

    Chandramohan, Laakshmana Sabari; Doolla, Suryanarayana; Khaparde, S. A.

    2016-02-01

    Perform Achieve Trade (PAT) is a market-based incentive mechanism to promote energy efficiency. The purpose of this work is to address the challenges inherent to inconsistent representation of business processes, and interoperability issues in PAT like cap-and-trade mechanisms especially when scaled. Studies by various agencies have highlighted that as the mechanism evolves including more industrial sectors and industries in its ambit, implementation will become more challenging. This paper analyses the major needs of PAT (namely tracking, monitoring, auditing & verifying energy-saving reports, and providing technical support & guidance to stakeholders); and how the aforesaid reasons affect them. Though current technologies can handle these challenges to an extent, standardization activities for implementation have been scanty for PAT and this work attempts to evolve them. The inconsistent modification of business processes, rules, and procedures across stakeholders, and interoperability among heterogeneous systems are addressed. This paper proposes the adoption of specifically two standards into PAT, namely Business Process Model and Notation for maintaining consistency in business process modelling, and Common Information Model (IEC 61970, 61968, 62325 combined) for information exchange. Detailed architecture and organization of these adoptions are reported. The work can be used by PAT implementing agencies, stakeholders, and standardization bodies.

  10. Howard Gardner Talks about Technology.

    ERIC Educational Resources Information Center

    Weiss, Ruth Palombo

    2000-01-01

    Howard Gardner advises teachers and trainers to use technology as a tool that can be used to educate, but one that shouldn't dictate educational goals. He suggests that goals must be determined before a particular technology can be embraced. Electronic media potentially will accommodate personal learning styles and intelligences. (JOW)

  11. Gladstone-Dale constant for CF4

    NASA Astrophysics Data System (ADS)

    Burner, A. W., Jr.; Goad, W. K.

    1980-05-01

    The Gladstone-Dale constant, which relates the refractive index to density, was measured for CF4 by counting fringes of a two-beam interferometer, one beam of which passes through a cell containing the test gas. The experimental approach and sources of systematic and imprecision errors are discussed. The constant for CF4 was measured at several wavelengths in the visible region of the spectrum. A value of 0.122 cu cm/g with an uncertainty of plus or minus 0.001 cu cm/g was determined for use in the visible region. A procedure for noting the departure of the gas density from the ideal-gas law is discussed.

  12. Astronaut Dale Gardner rehearses control of MMU during EVA practice

    NASA Technical Reports Server (NTRS)

    1984-01-01

    Astronaut Dale A. Gardner, 51-A mission specialist, rehearses control of manned maneuvering unit (MMU) during a practice for an extravehicular activity (EVA). Gardner is in the Shuttle mockup and integration laboratory at JSC.

  13. Water Quality Assessment Dale Hollow Lake and Its Inflows,

    DTIC Science & Technology

    1986-03-01

    NATIONAL BUREAU Of STANDARDS-1963-A I B US Army Corps of Engineers Nashville District Water Quality Assessment Dale Hollow Lake and Its Inflows March...PERIOD COVERED Water Quality Assessment Final Report Dale Hollow Lake and Its Inflows 6. PERFORMING ORO. REPORT NUMBER 7. AUTHOR(a) 6. CONTRACT OR... Water Resources Center, Tennessee Tech. Univ. Box 5082 Cookeville, TN 38505 11. CONTROLLING OFFICE NAME AND ADDRESS 12. REPORT DATE U.S. Army Engineer

  14. Howard University Assembles Fund-Raising Juggernaut

    ERIC Educational Resources Information Center

    Masterson, Kathryn

    2008-01-01

    As a dental student 35 years ago, Leo E. Rouse and his Howard University classmates learned to fill cavities and cap teeth by crowding around one faculty member and angling for a clear view of the day's demonstration. Today students at Howard's College of Dentistry, where Dr. Rouse is now the dean, get an unobstructed view of dental procedures…

  15. Howard Zinn and the Socially Conscious Academic

    ERIC Educational Resources Information Center

    McKivigan, John R.

    2009-01-01

    In recent decades many people came to know Howard Zinn for his outspoken advocacy on a wide range of progressive causes, including civil rights, free speech, workers' rights, education reform, and opposition to U.S. imperialism. The author's own first encounter with Howard Zinn's special combination of scholarship and activism occurred several…

  16. Oral Histories in Meteoritics and Planetary Science - XX: Dale Cruikshank

    NASA Astrophysics Data System (ADS)

    Sears, Derek W. G.

    2013-04-01

    In this interview, Dale Cruikshank (Fig. 1) explains how as an undergraduate at Iowa State University he was a summer student at Yerkes Observatory where he assisted Gerard Kuiper in work on his Photographic Lunar Atlas. Upon completing his degree, Dale went to graduate school at the University of Arizona with Kuiper where he worked on the IR spectroscopy of the lunar surface. After an eventful 1968 trip to Moscow via Prague, during which the Soviets invaded Czechoslovakia, Dale assumed a postdoc position with Vasili Moroz at the Sternberg Astronomical Institute and more observational IR astronomy. Upon returning to the United States and after a year at Arizona, Dale assumed a position at the University of Hawai'i that he held for 17 years. During this period Dale worked with others on thermal infrared determinations of the albedos of small bodies beyond the asteroid Main Belt, leading to the recognition that low-albedo material is prevalent in the outer solar system that made the first report of complex organic solids on a planetary body (Saturn's satellite Iapetus). After moving to Ames Research Center, where he works currently, he continued this work and became involved in many outer solar system missions. Dale has served the community through his involvement in developing national policies for science-driven planetary exploration, being chair of the DPS 1990-1991 and secretary/treasurer for 1982-1985. He served as president of Commission 16 (Physics of Planets) of the IAU (2001-2003). He received the Kuiper prize in 2006.

  17. 76 FR 53964 - Dale J. Bingham, P.A.; Revocation of Registration

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-08-30

    ... Enforcement Administration Dale J. Bingham, P.A.; Revocation of Registration On February 4, 2011, the Deputy... Show Cause to Dale J. Bingham, P.A. (Registrant), of Ash Fork, Arizona. The Show Cause Order proposed... Registration MB1048746, issued to Dale J. Bingham, P.A., be, and it hereby is, revoked. I further order...

  18. Dale Chihuly: An Inspiration in Art, Science, and Math!

    ERIC Educational Resources Information Center

    Hubbert, Beth

    2009-01-01

    Connecting students to the arts in a concrete way can be an effective teaching tool. In this article, the author describes how Dale Chihuly's "Hart Window," which features hand-blown glass disks affixed to the framework of the window, can be an inspiration for interdisciplinary connections in art, science and math. (Contains 4 online resources.)

  19. 105. Photocopy of plate opposite page 105 in Robert Dale ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    105. Photocopy of plate opposite page 105 in Robert Dale Owen, Hints on Public Architecture (New York, G. P. Putnam, 1849). GROUND-PLANS, SMITHSONIAN INSTITUTION - Smithsonian Institution Building, 1000 Jefferson Drive, between Ninth & Twelfth Streets, Southwest, Washington, District of Columbia, DC

  20. Aesthetics at the Impasse: The Unresolved Property of Dale Farm

    ERIC Educational Resources Information Center

    McCarthy, Lynne

    2015-01-01

    "Soil Depositions" was an art activist project that responded to the 2011 Irish Traveller eviction at Dale Farm in Essex when three resident women donated small amounts of soil from the site of their former home. The soil was subsequently deposited, framed and documented in various national and international locations. This article…

  1. Astronaut Dale Gardner holds up for sale sign after EVA

    NASA Technical Reports Server (NTRS)

    1984-01-01

    Astronaut Dale A. Gardner, having just completed the major portion of his second extravehicular activity (EVA) period in three days, holds up a 'for sale' sign. Astronaut Joseph P. ALlen IV, who also participated in the two EVA's, is reflected in Gardner's helmet visor. A portion of each of two recovered satellites is in the lower right corner, with Westar nearer Discovery's aft.

  2. Howard Hughes and His Colorful Aircraft Career

    ERIC Educational Resources Information Center

    Karwatka, Dennis

    2012-01-01

    The HK-1 "Hercules" airplane made its maiden flight over 60 years ago, and it still holds the record as the airplane with the largest wingspan that ever flew. Powered by eight massive 28-cylinder engines, it was piloted by Howard Hughes during its one brief flight in California. A large portion of the airplane was made of wood, which…

  3. Howard Zinn: Historian/Teacher as Citizen

    ERIC Educational Resources Information Center

    Jaramillo, Nathalia E.

    2009-01-01

    Few scholars have achieved the level of recognition and respect among the wider public as Howard Zinn. This should not come as a surprise, given the "unconventional" ways that Zinn embodied and enacted his scholarship. He often took his teaching and writing into those seemingly restricted spaces of popular protest, converting words into…

  4. Howard Parnes, MD | Division of Cancer Prevention

    Cancer.gov

    Dr. Howard Parnes received a BA from Cornell University in 1977 and an MD from the University of Medicine and Dentistry of New Jersey in 1981. He trained in internal medicine at the Johns Hopkins Bayview Medical Center from 1981 to 1984 followed by a medical oncology fellowship at the University of Maryland Cancer Center (UMCC) from 1984 to 1987. |

  5. Commentary: medicine, law, and Howard Zonana.

    PubMed

    Fox, Patrick K

    2010-01-01

    Collaboration between physicians and attorneys seems inherently unnatural because of the traditionally disparate aspects of the human condition that each addresses, with the health of mind and body the domain of Medicine, and the preservation of rights and property the considered realm of Law. At a time when it was not en vogue for physicians and attorneys to work together, Howard Zonana recognized the value of this partnership and fostered a model of collaboration from which society has greatly benefited.

  6. Gladstone-Dale constant for CF4. [experimental design

    NASA Technical Reports Server (NTRS)

    Burner, A. W., Jr.; Goad, W. K.

    1980-01-01

    The Gladstone-Dale constant, which relates the refractive index to density, was measured for CF4 by counting fringes of a two-beam interferometer, one beam of which passes through a cell containing the test gas. The experimental approach and sources of systematic and imprecision errors are discussed. The constant for CF4 was measured at several wavelengths in the visible region of the spectrum. A value of 0.122 cu cm/g with an uncertainty of plus or minus 0.001 cu cm/g was determined for use in the visible region. A procedure for noting the departure of the gas density from the ideal-gas law is discussed.

  7. Howard University Engineers Success: Interdisciplinary Study Keeps Howard on the Cutting Edge

    ERIC Educational Resources Information Center

    Chew, Cassie M.

    2004-01-01

    According to Engineering Workforce Commission annual reports, in 1999 Howard University graduated 108 students, 92 of whom were African American, in its chemical, civil, electrical, and mechanical engineering programs and computer science programs. After two more years of graduating approximately 100 students across programs, in 2002, according to…

  8. Howard H. Kendler (1919-2011).

    PubMed

    Foley, John M

    2012-09-01

    Presents an obituary for Howard H. Kendler. Kendler was born in New York City on June 9, 1919, and died in Santa Barbara, California, on February 17, 2011. Kendler majored in psychology at Brooklyn College, where he was an assistant to Abraham Maslow, the father of humanistic psychology, and did a research project on the psychology of thinking under the supervision of Solomon Asch, a leading Gestalt psychologist. Howard Kendler's career was characterized by insightful experiments and a consistent analysis of the fundamental issues of the nature of our science, its methods, and its role in the community. The breadth of his knowledge and the depth of his thinking on these issues are rare. Although he did not settle these issues, he brought them into clear focus and forcefully advocated for his point of view. He was a visiting professor at the University of California, Berkeley, Hebrew University in Jerusalem, and Tel-Aviv University and was a fellow at the Center for Advanced Study in the Behavioral Sciences. He held the offices of president of the Western Psychological Association, chairman of the Board of Governors of the Psychonomic Society, and president of the Division of General Psychology and the Division of Experimental Psychology of the American Psychological Association.

  9. Ecological risk assessment and sources of heavy metals in sediment from Daling River basin.

    PubMed

    Zhao, Lei; Mi, Dong; Chen, Yifu; Wang, Luo; Sun, Yeqing

    2015-04-01

    To investigate the distribution, source, and ecological risk of heavy metals in Daling River basin, 28 surface sediments collected in this region were analyzed by experimental and theoretical methods. Seven heavy metals, including Pb, Cr, Hg, Cu, As, Cd, and Zn, were detected in all samples. Monte Carlo simulation was used to assess the ecological risks of these heavy metals. It was found that the pollution of Cd was the most serious; the ecological risks in Daling River and Bohai Bay were significantly higher than those in estuary, Bohai Sea, and wetland, but overall, the ecological risks of these heavy metals were low to aquatic organisms in Daling River basin at present. Correlation analysis, principal component analysis, and cluster analysis showed that these heavy metals might originate from the same pollution sources located near Daling River and Bohai Bay.

  10. James Newton Howard: JAMs with TRI-M.

    ERIC Educational Resources Information Center

    Reninger, Rosemary D.

    2000-01-01

    Presents an interview with James Newton Howard, a film composer. Provides background information on Howard. Addresses topics such as his most challenging and rewarding scores, his musical background, and the benefits of being associated with the American Society of Composers, Authors, and Publishers (ASCAP). (CMK)

  11. Howard Beach Youth: A Study of Racial and Ethnic Attitudes.

    ERIC Educational Resources Information Center

    Lichter, Linda S.; Lichter, S. Robert

    This assessment of the climate of racial and ethnic attitudes in Howard Beach (New York) was conducted at John Adams High School, the public school attended by the greatest number of high school children in the Howard Beach community. The survey of 1,217 students was administered in December, 1986, several weeks before the incident in which a…

  12. Cecil H. "Pat" Patterson (1912-2006).

    PubMed

    Rose, Anthony L

    2007-09-01

    Cecil Holden "Pat" Patterson, 93, passed away on May 26, 2006, at his home in Asheville, North Carolina. A fellow of APA's Divisions of Counseling Psychology (17), Rehabilitation Psychology (22), and Psychotherapy (29), Pat was elected president of APA Division 17 in 1972. In recognition of his lifelong commitment to the field, he received the Division 17 Leona Tyler Award in 1994. Cecil H. "Pat" Patterson will be remembered as much for his love of family and the genuine personal interest he took in everyone he met as he will for his outstanding professional achievements.

  13. The correspondence of Thomas Dale (1700-1750): Botany in the transatlantic Republic of Letters.

    PubMed

    Cook, William J

    2012-03-01

    This paper seeks to provide a full account of the life and career of Dr. Thomas Dale (1700-1750), with particular reference to his botanical works and correspondence. Born in Hoxton, London, Dale studied medicine at Leiden and engaged fully in the social, literary and epistolary network in which botany was practised in eighteenth-century England. In 1730, however, Dale relocated to the British colonial port of Charles Town, South Carolina. Here he continued to engage in a transatlantic network of botanical exchange and discussion, corresponding on equal and reciprocal terms with his former colleagues in England. Where Dale differs from naturalists in South Carolina before him is that his motives for pursuing botany and for corresponding with English naturalists were located firmly in the New World. Such a conclusion forms a valuable, albeit small contribution to models for the development of national scientific cultures in the imperial world. Similarly, Dale's pursuit of botanical information in South Carolina provides a small amount of material with which to illustrate currently fashionable models for the mediated exchange and circulation of scientific knowledge.

  14. Dale Reed with model in front of M2-F1

    NASA Technical Reports Server (NTRS)

    1967-01-01

    Dale Reed with a model of the M2-F1 in front of the actual lifting body. Reed used the model to show the potential of the lifting bodies. He first flew it into tall grass to test stability and trim, then hand-launched it from buildings for longer flights. Finally, he towed the lifting-body model aloft using a powered model airplane known as the 'Mothership.' A timer released the model and it glided to a landing. Dale's wife Donna used a 9 mm. camera to film the flights of the model. Its stability as it glided--despite its lack of wings--convinced Milt Thompson and some Flight Research Center engineers including the center director, Paul Bikle, that a piloted lifting body was possible. The lifting body concept evolved in the mid-1950s as researchers considered alternatives to ballistic reentries of piloted space capsules. The designs for hypersonic, wingless vehicles were on the boards at NASA Ames and NASA Langley facilities, while the US Air Force was gearing up for its Dyna-Soar program, which defined the need for a spacecraft that would land like an airplane. Despite favorable research on lifting bodies, there was little support for a flight program. Dryden engineer R. Dale Reed was intrigued with the lifting body concept, and reasoned that some sort of flight demonstration was needed before wingless aircraft could be taken seriously. In February 1962, he built a model lifting body based upon the Ames M2 design, and air-launched it from a radio controlled 'mothership.' Home movies of these flights, plus the support of research pilot Milt Thompson, helped pursuade the facilities director, Paul Bikle, to give the go-ahead for the construction of a full-scale version, to be used as a wind-tunnel model and possibly flown as a glider. Comparing lifting bodies to space capsules, an unofficial motto of the project was, 'Don't be Rescued from Outer Space--Fly Back in Style.' The construction of the M2-F1 was a joint effort by Dryden and a local glider manufacturer, the

  15. 15. Historic American Buildings Survey Photocopy, C. Howard Crane Office ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    15. Historic American Buildings Survey Photocopy, C. Howard Crane Office Drawing of 1919. Courtesy Smith, Hinchman & Grylls Inc., Detroit, 1973 BASEMENT PLAN - Orchestra Hall, 3711 Woodward Avenue, Detroit, MI

  16. Obituary: Howard H. Lanning, 1946-2007

    NASA Astrophysics Data System (ADS)

    Wade, Richard A.; MacConnell, D. Jack

    2009-01-01

    Howard H. Lanning died 20 December 2007 in Tucson, Arizona. He was a Software Quality Assurance Engineer for the Data Products Program at the National Optical Astronomy Observatory [NOAO] in Tucson, having returned to his native West after twenty years at the Space Telescope Science Institute [STScI] in Baltimore, Maryland. Throughout his career in roles supporting other astronomers, Howard steadily and persistently worked at his own research programs, most notably the identification and study of UV-bright sources in the Sandage Two-Color Survey of the Galactic Plane. Minor planet 2000 QJ248 has been named (61913) Lanning in recognition of his contributions. The son of James Clyde Lanning and Ethel Malan Lanning, Howard was born 26 May 1946, in Brawley, a small community near Calipatria, California. His parents worked for the local school district where his late father was Superintendent of Maintenance and Grounds and his mother was a school principal. After graduating from high school in a class of 52, Howard earned his A.A. degree at Imperial Valley Junior College, majoring in Astronomy and minoring in Mathematics. He completed his A.B. degree in Applied Arts and Sciences at San Diego State University [SDSU] in 1969 with the same major and minor. In 1974, he completed his M.S. in Astronomy at SDSU with a thesis on the period variation of the white-dwarf eclipsing binary BD +16∘516. During his time at SDSU Lanning was a Research Assistant/Observer at the former Hale Observatories, working on the optical identification of X-ray sources under the direction of Allan Sandage. His second refereed paper, the first of several finding lists of UV-bright stars arising from this work, appeared in 1973. Fellow students and faculty remember that he was a self-starter, initiating many observing projects of his own at Mount Laguna Observatory and encouraging others to do the same. For more than a decade Lanning was an observer and night assistant at Mount Wilson Observatory

  17. Perfluoroalkyl substances in Daling River adjacent to fluorine industrial parks: implication from industrial emission.

    PubMed

    Meng, Jing; Wang, Tieyu; Wang, Pei; Zhu, Zhaoyun; Li, Qifeng; Lu, Yonglong

    2015-01-01

    The pollution level and source of perfluoroalkyl substances (PFASs) in mainstream and tributary of Daling River in northeast China were investigated in present study. Concentrations of PFASs in surface water and sediment ranged from 4.6 to 3,410 ng/L and from 0.08 to 2.6 ng/g dry weight, respectively. The lowest levels of PFASs were found in vicinity of a drinking water source located in upstream of Daling River. Xihe tributary, which is adjacent to two local fluorine industrial parks, contained the highest level of PFASs. Short-chain PFASs, including perfluorobutanoic acid and perfluorobutane sulfonate, were of higher levels due to their emerging as alternative products for perfluorooctane sulfonate. High level of perfluorooctanoic acid was also found in Daling River. Based on these results, it can be concluded that the relatively severe pollutions of Xihe tributary were caused by long-term development of the two local fluorine industry parks.

  18. Dale Avenue Performance Objective Model Pre-Primary-Primary Performance Objectives Prekindergarten Through Grade Three: Manual; Record Book.

    ERIC Educational Resources Information Center

    Hanson, Helen B.; Gavzy, Rita

    The Dale Avenue Early Childhood Education Project was developed in the Dale Avenue School in Paterson, New Jersey through funding from the Elementary Secondary Education Act Title III. The Project was validated in 1973 by the standards and guidelines of the U.S. Office of Education as innovative, successful, cost effective, and exportable. As a…

  19. A Poetry Workshop in Print: Pat Mora

    ERIC Educational Resources Information Center

    Hopkins, Lee Bennett

    2006-01-01

    After a successful career as a writer for adults, Pat Mora began creating books for children. Her first picture book, "Tomas and The Library Lady" (Knopf, 1997) is a tender story of a young migrant worker who unearths new worlds when he discovers the magic a public library holds. The text, cleverly interspersed with foreign words, became a…

  20. Antimatter/HiPAT Support Services

    NASA Technical Reports Server (NTRS)

    Lewis, Raymond A.

    2001-01-01

    Techniques were developed for trapping normal matter in the High Performance Antiproton Trap (HiPAT). Situations encountered included discharge phenomena, charge exchange and radial diffusion processes. It is important to identify these problems, since they will also limit the performance in trapping antimatter next year.

  1. Speaking Out against the Silence: Pat Griffin.

    ERIC Educational Resources Information Center

    Portman, Penelope A.; Carlson, Teresa B.

    1991-01-01

    Discusses cultural diversity from the point of view of Pat Griffin, a lesbian feminist and associate professor of physical education. To her, cultural diversity includes religion, race, gender, physical and mental ability, class, and sexual orientation. She emphasizes the latter because it is often ignored in the school environment. (SM)

  2. Dewey, Dale, and Bruner: Educational Philosophy, Experiential Learning, and Library School Cataloging Instruction.

    ERIC Educational Resources Information Center

    Garrett, LeAnn

    1997-01-01

    Discusses educational philosophies of John Dewey, Edgar Dale, and Jerome Bruner dealing with experience and learning and describes experiential learning activities within the curriculum of the School of Library and Information Studies at the University of Hawaii. Results of a study examining student attitudes toward experiential learning are…

  3. Astronaut Dale Gardner using MMU to travel to Westar VI satellite

    NASA Technical Reports Server (NTRS)

    1984-01-01

    Astronaut Dale A. Gardner, wearing the manned maneuvering unit (MMU) approaching the spinng Westar VI satellite over Bahama Banks. Gardner uses a large tool called the apogee kick motor capture device (ACD) to enter the nozzle of the spent Westar engine and stabilize the satellite to capture it for return to Earth.

  4. Astronaut Dale Gardner using MMU to travel to Westar VI satellite

    NASA Technical Reports Server (NTRS)

    1984-01-01

    Astronaut Dale A. Gardner, wearing the manned maneuvering unit (MMU) approaching the spinng Westar VI satellite over Bahama Banks. The end effector of the remote manipulator system (RMS) controlled by Dr. Anna L. Fisher inside Discovery's cabin, awaits its duty at right.

  5. Astronat Dale Gardner using MMU to travel to Westar VI satellite

    NASA Technical Reports Server (NTRS)

    1984-01-01

    Astronat Dale A. Gardner appears to be under the remote manipulator system (RMS) end effector as he makes a turn in space and preapares to use the manned maneuvering unit (MMU) to travel to Westar VI satellite. The 'stinger' he carries will enter the communications satellite through the nozzle of the spent motor.

  6. Previous Attempts to Debunk the Mythical Retention Chart and Corrupted Dale's Cone

    ERIC Educational Resources Information Center

    Subramony, Deepak Prem; Molenda, Michael; Betrus, Anthony K.; Thalheimer, Will

    2014-01-01

    Critics have been attempting to debunk the mythical retention chart at least since 1971. The earliest critics, David Curl and Frank Dwyer, were addressing just the retention data. Beginning around 2002, a new generation of critics has taken on the illegitimate combination of the retention chart and Edgar Dale's Cone of Experience--the corrupted…

  7. PatA and PatB form a functional heterodimeric ABC multidrug efflux transporter responsible for the resistance of Streptococcus pneumoniae to fluoroquinolones.

    PubMed

    Boncoeur, Emilie; Durmort, Claire; Bernay, Benoît; Ebel, Christine; Di Guilmi, Anne Marie; Croizé, Jacques; Vernet, Thierry; Jault, Jean-Michel

    2012-10-02

    All bacterial multidrug ABC transporters have been shown to work as either homodimers or heterodimers. Two possibly linked genes, patA and patB from Streptococcus pneumococcus, that encode half-ABC transporters have been shown previously to be involved in fluoroquinolone resistance. We showed that the ΔpatA, ΔpatB, or ΔpatA/ΔpatB mutant strains were more sensitive to unstructurally related compounds, i.e., ethidium bromide or fluoroquinolones, than the wild-type R6 strain. Inside-out vesicles prepared from Escherichia coli expressing PatA and/or PatB transported Hoechst 33342, a classical substrate of multidrug transporters, only when both PatA and PatB were coexpressed. This transport was inhibited either by orthovanadate or by reserpine, and mutation of the conserved Walker A lysine residue of either PatA or PatB fully abrogated Hoechst 33342 transport. PatA, PatB, and the PatA/PatB heterodimer were purified from detergent-solubilized E. coli membrane preparations. Protein dimers were identified in all cases, albeit in different proportions. In contrast to the PatA/PatB heterodimers, homodimers of PatA or PatB failed to show a vanadate-sensitive ATPase activity. Thus, PatA and PatB need to interact together to make a functional drug efflux transporter, and they work only as heterodimers.

  8. Rise of Human Intelligence: Comments on Howard (1999).

    ERIC Educational Resources Information Center

    Gobet, Fernand; Campitelli, Guillermo; Waters, Andrew J.

    2002-01-01

    Finds several difficulties with the theory advanced by R. Howard and shows that alternative explanations relating to changes in the chess environment, including increased access to chess knowledge, offer better explanations for the increased presence of young players at top-level chess. (SLD)

  9. Evaluation of the Howard Hughes Science Grant Project, Year One

    ERIC Educational Resources Information Center

    Wolanin, Natalie; Wade, Julie

    2015-01-01

    The goal of the Howard Hughes Science Institute (HHMI) supported science program is to train one staff member to become a science lead within each of the elementary schools in the Montgomery County (Maryland) Public Schools (MCPS) district. The specific objectives of the first year of HHMI grant project were to: (1) provide approximately 20…

  10. A Review of Howard University's Financial Literacy Curriculum

    ERIC Educational Resources Information Center

    Lindsey-Taliefero, Debby; Kelly, Lynne; Brent, William; Price, Russell

    2011-01-01

    This article evaluates a financial literacy curriculum at the Howard University (HU) School of Business, by measuring the financial knowledge acquired after participating in a variety of programs. To evaluate the HU curriculum, the National Jump$tart Coalition (NJC) survey was administered to collect data on financial knowledge and demographic…

  11. Judicial Management: The Achievements of Chief Justice William Howard Taft.

    ERIC Educational Resources Information Center

    Post, Robert

    1998-01-01

    Illuminates the importance of Chief Justice William Howard Taft in creating the modern administrative role of the Chief Justice of the United States. Specifically, the article examines the Act of 14 September 1922 that Taft championed in Congress to give the Chief Justice better tools for managing the judiciary. (DSK)

  12. B & P Junction Substation & Interlocking Tower; Howard Street ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    B & P Junction Substation & Interlocking Tower; Howard Street Bridge. Baltimore, Baltimore City, MD. Sec. 1201, MP 95.85 - Northeast Railroad Corridor, Amtrak route between District of Columbia/Maryland state line & Maryland/Delaware state line, Baltimore, Independent City, MD

  13. Undue Certainty: Where Howard Zinn's "A People's History" Falls Short

    ERIC Educational Resources Information Center

    Wineburg, Sam

    2013-01-01

    Howard Zinn's "A People's History" of the United States has few peers among contemporary historical works. With more than 2 million copies in print, "A People's History" is more than a book. It is a cultural icon. While most historians aim to examine the full historical record, Zinn picks and chooses from it. Writing persuasively, he hides the…

  14. A tribute to Robert Edwards and Howard Jones Jr

    PubMed Central

    Ombelet, W.

    2011-01-01

    “2010 was a fascinating year. Robert Edwards finally received the Nobel prize for Medicine and his friend in the United States, Howard W. Jones Jr. was honored in Denver by the American Society of Reproductive Medicine (ASRM) upon his Centennial Birthday. He turned 100 on December 30th” PMID:24753845

  15. Composing a "National Negro Theater": Playwriting Courses at Howard University in the 1920s.

    ERIC Educational Resources Information Center

    Zaluda, Scott

    Educators today may find a historical review of the Howard Players at Howard University (Washington, D.C.) in the 1920s important because of its implicit commentary on what constitutes community. While the Howard Players are generally written about in terms of the development of an African-American theater, historians ought also to think of their…

  16. 77 FR 29456 - Central Railroad Company of Indianapolis-Abandonment Exemption-in Howard County, IN

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-05-17

    ... Surface Transportation Board Central Railroad Company of Indianapolis--Abandonment Exemption-- in Howard... Industrial Lead, between mileposts 55.66 and 58.5 in Howard County, Ind.\\1\\ The line traverses United States...--Abandonment Exemption--in Howard County, Ind., AB 511 (Sub-No. 5X) (STB served July 22, 2010)....

  17. Role of gibberellins in parthenocarpic fruit development induced by the genetic system pat-3/pat-4 in tomato.

    PubMed

    Fos, Mariano; Proaño, Karina; Nuez, Fernando; García-Martínez, José L.

    2001-04-01

    The role of gibberellins (GAs) in the induction of parthenocarpic fruit-set and growth by the pat-3/pat-4 genetic system in tomato (Lycopersicon esculentum Mill.) was investigated using wild type (WT; Cuarenteno) and a near-isogenic line derived from the German line RP75/59 (the source of pat-3/pat-4 parthenocarpy). Unpollinated WT ovaries degenerated but GA3 application induced parthenocarpic fruit growth. On the contrary, parthenocarpic growth of pat-3/pat-4 fruits, which occurs in the absence of pollination and hormone treatment, was not affected by applied GA3. Unpollinated pat-3/pat-4 fruit growth was negated by paclobutrazol, an inhibitor of ent-kaurene oxidase, and this inhibitory effect was negated by GA3. The quantification of the main GAs of the early 13-hydroxylation pathway (GA1, GA8, GA19, GA20, GA29 and GA44) in unpollinated ovaries at 3 developmental stages (flower bud, FB; pre-anthesis, PR; and anthesis, AN), by gas chromatography-selected ion monitoring, showed that the concentration of most of them was higher in pat-3/pat-4 than in WT ovaries at PR and AN stages. The concentration of GA1, suggested previously to be the active GA in tomate, was 2-4 times higher. Unpollinated pat-3/pat-4 ovaries at FB, PR and AN stages also contained relatively high amounts (5-12 ng g-1) of GA3, a GA found at less than 0.5 ng g-1 in WT ovaries. It is concluded that the mutations pat-3/pat-4 may induce natural facultative parthenocarpy capacity in tomato by increasing the concentration of GA1 and GA3 in the ovaries before pollination.

  18. The evaluation of Pat-Pat related injuries in the western black sea region of Turkey

    PubMed Central

    2011-01-01

    Background Accidents caused by motorized vehicle in the agricultural sector are frequently observed. In Turkey; accidents arising from motorized vehicles, named Pat-Pat, which are used by farmers in the Western Black Sea region is not unusual. Methods One hundred five patients who were brought into the Emergency Department of Duzce University, Medical Faculty Hospital between September 2009 and August 2010 due to Pat-Pat related accidents were evaluated. Results The cases consisted of 73 (69.5%) males and 32 (30.5%) females, ranging from 2 to 73 years of age. In the 10-39 age group, a total of 63 (60.0%) cases were determined. The months when the greatest rate of cases applied to the hospital consisted of July, August, September and the season is summer. The cases were exposed to trauma in roads in 54 (51.4%), and 51 (48.6%) occurred in agricultural area without roads. Eighty seven (82.9%) cases were injured due to the overturning of vehicle. The patients were brought to the hospital using a private vehicle in 54 (51.4%) of the cases and in 51 (48.6%) cases, 112 ambulance system was used. The cases were determined to apply to the hospital most frequently between 6 pm-12 am. The injuries frequently consisted of head-neck and spine traumas, thorax traumas and upper extremity traumas. In 55 (52.4%) cases, open wound-laceration was determined. Seventy five (71.4%) cases were treated in the Emergency Department, and 28 (26.7%) were hospitalized. Three (2.9%) cases were deceased. Conclusions Serious injuries can occur in Pat-Pat related accidents, and careful systematic physical examination should be conducted. In order to prevent these accidents, education of farm operators and engineering studies on the mechanics and safety of these vehicles should be taken and legal regulations should be created. PMID:21699689

  19. STS-105 Crew Interview: Pat Forrester

    NASA Technical Reports Server (NTRS)

    2001-01-01

    STS-105 Mission Specialist Pat Forrester is seen during a prelaunch interview. He answers questions about his inspiration to become an astronaut, his career path, training for the mission, and his role in the mission's activities. He gives details on the mission's goals, which include the transfer of supplies from the Discovery Orbiter to the International Space Station (ISS) and the change-over of the Expedition 2 and Expedition 3 crews (the resident crews of ISS). Forrester discusses the importance of the ISS in the future of human spaceflight.

  20. PAT-1 safety analysis report addendum.

    SciTech Connect

    Weiner, Ruth F.; Schmale, David T.; Kalan, Robert J.; Akin, Lili A.; Miller, David Russell; Knorovsky, Gerald Albert; Yoshimura, Richard Hiroyuki; Lopez, Carlos; Harding, David Cameron; Jones, Perry L.; Morrow, Charles W.

    2010-09-01

    The Plutonium Air Transportable Package, Model PAT-1, is certified under Title 10, Code of Federal Regulations Part 71 by the U.S. Nuclear Regulatory Commission (NRC) per Certificate of Compliance (CoC) USA/0361B(U)F-96 (currently Revision 9). The purpose of this SAR Addendum is to incorporate plutonium (Pu) metal as a new payload for the PAT-1 package. The Pu metal is packed in an inner container (designated the T-Ampoule) that replaces the PC-1 inner container. The documentation and results from analysis contained in this addendum demonstrate that the replacement of the PC-1 and associated packaging material with the T-Ampoule and associated packaging with the addition of the plutonium metal content are not significant with respect to the design, operating characteristics, or safe performance of the containment system and prevention of criticality when the package is subjected to the tests specified in 10 CFR 71.71, 71.73 and 71.74.

  1. Dr. Howard Jones, Jr. (1910-2015): In Memoriam

    PubMed Central

    Oehninger, S.; Kruger, T.F.

    2015-01-01

    We honor the life of Howard W. Jones, Jr., MD, 104, a pioneer and visionary in reproductive medicine, a dedicated mentor, internationally renowned gynecological surgeon, and the father of in vitro fertilization IVF) in the United States. Dr. Jones founded the Jones Institute for Reproductive Medicine, in Norfolk, Virginia, at Eastern Virginia Medical School, in 1983. Today, IVF has grown exponentially not only in its direct application but also in the development of newer techniques would have been unimaginable a couple of decades ago. Throughout the years Dr. Howard has been a teacher and a mentor to many around the world. To us he also was a friend, a supporter at the time of difficult decisions, someone who would inspire hope, ignite the imagination, and instill a love of learning and untangling some of the complexities of the field, even of the human condition. His leadership, vision, knowledge, enthusiasm and inquisitive mind will be missed.

  2. Temperature Analysis: Howard A. Hanson Reservoir, Washington. Mathematical Model Investigation

    DTIC Science & Technology

    1988-09-01

    were reached by Dortch (1981) in his investigation of Kinzua Dam in Pennsylvania and by Peters (1978) in his report of modifications to Flaming Gorge ...Vicksburg, MS. Peters, J. C. 1978. "Modification of Intakes at Flaming Gorge Dam , Utah, to Improve Water Temperature in the Green River," Proceedings...system to maintain downstream temperature objectives. Project Description 2. Howard A. Hanson Dam is located 65 miles upstream from the mouth of the

  3. Differential expression of proton-assisted amino acid transporters (PAT[1] and PAT[2]) in tissues of neonatal pigs

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The PATs have been identified as growth-regulatory nutrient sensors in Drosophila and as activators of mammalian target of rapamycin (mTOR) in mammalian cell cultures. These studies suggest that, beyond their classical function as transporters of simple amino acids (AA), the PATs act as tranceptors,...

  4. Pat Thiel talks about Nobel Prize winner Dan Shechtman

    SciTech Connect

    Thiel, Pat

    2012-01-01

    Ames Laboratory senior scientist and Iowa State University Distinguished Professor of Chemistry Pat Thiel talks about her friend and colleague Dan Shechtman who received the 2011 Nobel Prize for Chemistry.

  5. Pat Thiel talks about Nobel Prize winner Dan Shechtman

    ScienceCinema

    Thiel, Pat

    2016-07-12

    Ames Laboratory senior scientist and Iowa State University Distinguished Professor of Chemistry Pat Thiel talks about her friend and colleague Dan Shechtman who received the 2011 Nobel Prize for Chemistry.

  6. Start/Pat; A parallel-programming toolkit

    SciTech Connect

    Appelbe, B.; Smith, K. ); McDowell, C. )

    1989-07-01

    How can you make Fortran code parallel without isolating the programmer from learning to understand and exploit parallelism effectively. With an interactive toolkit that automates parallelization as it educates. This paper discusses the Start/Pat toolkit.

  7. Cosmopolitanism and Our Descriptions of Ethics and Ontology: A Response to Dale Snauwaert's "The Ethics and Ontology of Cosmopolitanism"

    ERIC Educational Resources Information Center

    Hansen, David T.

    2010-01-01

    In recent years scholars across the humanities and social sciences have revitalized the ancient concept of cosmopolitanism. Dale Snauwaert illuminates why this is so in his thoughtful article on what it might mean to educate for a shared humanity. Snauwaert shows why many people find so-called "realism" an unsatisfactory political and moral…

  8. Macquarie Island: the introduction of the European rabbit flea Spilopsyllus cuniculi (Dale) as a possible vector for myxomatosis

    PubMed Central

    Sobey, W. R.; Adams, K. M.; Johnston, G. C.; Gould, L. R.; Simpson, K. N. G.; Keith, K.

    1973-01-01

    The European rabbit flea Spilopsyllus cuniculi (Dale) was first released on Macquarie Island in December 1968. The flea has survived and bred on the island and about 30% of the rabbits sampled from the original release area in January 1972 were flea-infested. ImagesPlate 1Plate 1 PMID:4515880

  9. Howard's End: A Narrative Memoir of Political Contrivance, Neoconservative Ideology and the Australian History Curriculum

    ERIC Educational Resources Information Center

    Taylor, Tony

    2009-01-01

    In August 2006, Australia's conservative prime minister John Howard convened a history summit in Canberra. The purported goal of the summit was the framing of a nationally-acceptable curriculum in Australian history. However, as this article suggests, Howard's hidden intention was to use the summit as a device for introducing a narrowly…

  10. Overexpression of patA and patB, which encode ABC transporters, is associated with fluoroquinolone resistance in clinical isolates of Streptococcus pneumoniae.

    PubMed

    Garvey, Mark I; Baylay, Alison J; Wong, Ryan L; Piddock, Laura J V

    2011-01-01

    Fifty-seven clinical isolates of Streptococcus pneumoniae were divided into four groups based on their susceptibilities to the fluoroquinolones ciprofloxacin and norfloxacin and the dyes ethidium bromide and acriflavine. Comparative reverse transcription-PCR was used to determine the level of expression of the genes patA and patB, which encode putative ABC transporters. Overexpression was observed in 14 of the 15 isolates that were resistant to both fluoroquinolones and dyes and in only 3 of 24 of those resistant to fluoroquinolones only. Isolates overexpressing patA and patB accumulated significantly less of the fluorescent dye Hoechst 33342 than wild-type isolates, suggesting that PatA and PatB are involved in efflux. Inactivation of patA and patB by in vitro mariner mutagenesis conferred hypersusceptibility to ethidium bromide and acriflavine in all isolates tested and lowered the MICs of ciprofloxacin in the patAB-overproducing and/or fluoroquinolone-resistant isolates. These data represent the first observation of overexpression of patA and patB in clinical isolates and show that PatA and PatB play a clinically relevant role in fluoroquinolone resistance.

  11. The Planetary and Eclipse Oil Paintings of Howard Russell Butler

    NASA Astrophysics Data System (ADS)

    Pasachoff, Jay M.; Olson, R. M.

    2013-10-01

    The physics-trained artist Howard Russell Butler (1856-1934) has inspired many astronomy students through his planetary and eclipse paintings that were long displayed at the Hayden Planetarium in New York, the Fels Planetarium at the Franklin Institute in Philadelphia, and the Buffalo Museum of Science. We discuss not only the eclipse triptychs (1918, 1923, and 1925) at each of those institutions but also his paintings of Mars as seen from Phobos and from Deimos (with landscapes of those moons in the foreground depicted in additional oils hung at Princeton University) and the Earth from our Moon. We also describe his involvement with astronomy and his unique methodology that allowed him to surpass the effects then obtainable with photography, as well as his inclusion in a U.S. Naval Observatory eclipse expedition in 1918, as well as his auroral, solar-prominence, and 1932-eclipse paintings.

  12. Database of patterns PROF_PAT for detecting local similarities.

    PubMed

    Nizolenko, Lily P; Bachinsky, Alexander G; Naumochkin, Andrey N; Yarigin, Andrey A; Grigorovich, Dmitry A

    2003-01-01

    We have developed PROF_PAT, a database of patterns, constructed for groups of related proteins and designed to maximize representation of amino acid sequences from the SWISS-PROT database. The purpose of the current study was to demonstrate that PROT_PAT is not only as good as known analogs but surpasses them in some features. 10938 new amino acid sequences from the SWISS-PROT bank were compared with patterns constructed for protein families in the PROF_PAT 1.10 bank. The aim of the comparisons was to estimate some threshold values of "Score" parameter to distinguish random similarities from significant ones. From the 10938 new sequences, 638 did not reveal any similarities with PROF_PAT patterns. Cases of found similarities were divided into three sets: 'positive', 'putative' (or 'unknown'), and 'false positive', containing 7719, 2297 and 284 sequences respectively. Using 20 amino acid sequences from the TrEMBL bank that have no descriptions, PROF_PAT demonstrated specificity at a level that was as good as the best-known "secondary" banks. At the same time, its pattern content and variety of included proteins was significantly richer, and its search speed was 3-10 times higher than those of any other protein family bank used for comparison.

  13. Unto the least of these: the Howard Association and yellow fever.

    PubMed

    Newsom, E Y

    1992-06-01

    Epidemics of yellow fever in mid-19th century America caused, in the port cities of the South, devastation and death almost unequalled in this country's history. In response to this horror, a benevolent organization of young men was formed to minister to the unfortunate victims through visitations, nursing care, supplies, and compassion. The group adopted the name Howard Association in honor of the British philanthropist and reformer, John Howard. This paper is an attempt to introduce this little-known society to 20th century readers by taking a brief look at some of the records of Howard Associations in several southern cities: New Orleans, Memphis, Norfolk, and Charleston.

  14. Modelling the effects of stranding on the Atlantic salmon population in the Dale River, Norway.

    PubMed

    Sauterleute, Julian F; Hedger, Richard D; Hauer, Christoph; Pulg, Ulrich; Skoglund, Helge; Sundt-Hansen, Line E; Bakken, Tor Haakon; Ugedal, Ola

    2016-12-15

    Rapid dewatering in rivers as a consequence of hydropower operations may cause stranding of juvenile fish and have a negative impact on fish populations. We implemented stranding into an Atlantic salmon population model in order to evaluate long-term effects on the population in the Dale River, Western Norway. Furthermore, we assessed the sensitivity of the stranding model to dewatered area in comparison to biological parameters, and compared different methods for calculating wetted area, the main abiotic input parameter to the population model. Five scenarios were simulated dependent on fish life-stage, season and light level. Our simulation results showed largest negative effect on the population abundance for hydropeaking during winter daylight. Salmon smolt production had highest sensitivity to the stranding mortality of older juvenile fish, suggesting that stranding of fish at these life-stages is likely to have greater population impacts than that of earlier life-stages. Downstream retention effects on the ramping velocity were found to be negligible in the stranding model, but are suggested to be important in the context of mitigation measure design.

  15. Dale Schumacher on strategic quality planning and research. Interview by Joann Grenovich-Richards.

    PubMed

    Schumacher, Dale

    2003-01-01

    Dale N. Schumacher, MD MPH, is president of the Rockburn Institute, a not-for-profit health services research and consulting organization. Dr. Schumacher has 25 years' experience in the fields of medicine, health services research, and consulting. He spent 3 years in the U.S. Public Health Service, where he coordinated the development of the Sickness Index Profile, an early health status measure. Dr. Schumacher also helped establish the Center for Hospital Finance and Management at The Johns Hopkins Medical Institutions, where he was an associate director. He has held senior management positions or consulted with the Sisters of Mercy Health Corporation, Holy Cross Health System, Alexian Brothers Health System, Catholic Medical Center, and Northwestern Healthcare. Currently, he is senior medical advisor to the National Business Coalition on Health and serves as clinical informatics officer for Crozer-Keystone Health System in Philadelphia. He is a board member at Kaleida Health System, Buffalo, NY. Dr. Schumacher has written more than 40 publications in the areas of medical education, medical care, quality-of-care assessment, healthcare data analysis, and reliability of chart-review data. Dr. Schumacher earned his medical degree from the University of Illinois and his master of public health degree from The Johns Hopkins University School of Hygiene and Public Health, and was a postdoctoral fellow there in the departments of health services administration and epidemiology. He trained at The Johns Hopkins/Baltimore City Hospitals and St. Agnes Hospital. Dr. Schumacher is board certified in internal medicine.

  16. Dynamic recrystallization mechanisms and their transition in the Daling Thrust (DT) zone, Darjeeling-Sikkim Himalaya

    NASA Astrophysics Data System (ADS)

    Ghosh, Subhajit; Bose, Santanu; Mandal, Nibir; Dasgupta, Sujoy

    2016-04-01

    The Daling Thrust (DT) delineates a zone of intense shear localization in the Lesser Himalayan Sequence (LHS) of the Darjeeling-Sikkim Himalaya. From microstructural studies of deformed quartzite samples, we show a transition in the dynamic recrystallization mechanism with increasing distance from the DT, dominated by grain boundary bulging (BLG) recrystallization closest to the DT, and progressively replaced by sub-grain rotation (SGR) recrystallization away from the thrust. The transition is marked by a characteristic variation in the fractal dimension (D) of grain boundaries, estimated from the area-perimeter method. For the BLG regime, D ≈ 1.046, which decreases significantly to a value as low as 1.025 for the SGR regime. Using the available thermal data for BLG and SGR recrystallization, we infer increasing deformation temperatures away from the DT in the hanging wall. Based on the quartz piezometer our estimates reveal strong variations in the flow stress (59.00 MPa to 16.00 MPa) over a distance of ~ 1.2 km from the DT. Deformation mechanism maps constructed for different temperatures indicate that the strain rates (10- 12 S- 1 to 10- 14 S- 1) comply with the geologically possible range. Finally, we present a mechanical model to provide a possible explanation for the cause of stress intensification along the DT.

  17. Commission on the Future of Howard Community College, Progress Report 2000.

    ERIC Educational Resources Information Center

    Howard Community Coll., Columbia, MD. Office of Planning and Evaluation.

    Presents the Commission on the Future of Howard Community College's (HCC) (Maryland) progress report 2000. Section 1, "Creating a World Class Learning Organization," presents strategic process and tactical action recommendations. Section 2, "Collaboration with Other Educational Organizations," discusses curricular and…

  18. The Solar Eclipse Mural Series by Howard Russell Butler

    NASA Astrophysics Data System (ADS)

    Pasachoff, J. M.; Olson, R. J. M.

    2016-01-01

    There is a rich trove of astronomical phenomena in works of art by artists from the greater New York area, a trend that is even more pronounced in the oeuvres of New York City residents through the present day. A case in point is the trio of oil paintings by artist (and former physics professor) Howard Russell Butler depicting total solar eclipses in 1918, 1923, and 1925 that are based on his own observations. They were long displayed in the former art-deco building of the Hayden Planetarium of the American Museum of Natural History, the location of this conference. (The Museum also has nine other Butler paintings, none of which are currently exhibited.) Since the eclipse paintings have been in storage for many years, these once famous works are now virtually forgotten. Based on our research as an astronomer who has seen sixty-two solar eclipses and an art historian who has written extensively about astronomical imagery, we will discuss Butler's Solar Eclipse Triptych to explore its place in the history of astronomical imaging.

  19. Howard University Energy Expert Systems Institute Summer Program (EESI)

    NASA Technical Reports Server (NTRS)

    Momoh, James A.; Chuku, Arunsi; Abban, Joseph

    1996-01-01

    Howard University, under the auspices of the Center for Energy Systems and Controls runs the Energy Expert Systems Institute (EESI) summer outreach program for high school/pre-college minority students. The main objectives are to introduce precollege minority students to research in the power industry using modern state-of-the-art technology such as Expert Systems, Fuzzy Logic and Artificial Neural Networks; to involve minority students in space power management, systems and failure diagnosis; to generate interest in career options in electrical engineering; and to experience problem-solving in a teamwork environment consisting of faculty, senior research associates and graduate students. For five weeks the students are exposed not only to the exciting experience of college life, but also to the inspiring field of engineering, especially electrical engineering. The program consists of lectures in the fundamentals of engineering, mathematics, communication skills and computer skills. The projects are divided into mini and major. Topics for the 1995 mini projects were Expert Systems for the Electric Bus and Breast Cancer Detection. Topics on the major projects include Hybrid Electric Vehicle, Solar Dynamics and Distribution Automation. On the final day, designated as 'EESI Day' the students did oral presentations of their projects and prizes were awarded to the best group. The program began in the summer of 1993. The reaction from the students has been very positive. The program also arranges field trips to special places of interest such as the NASA Goddard Space Center.

  20. Howard Russell Butler's Oil Paintings of Solar Eclipses and Prominences

    NASA Astrophysics Data System (ADS)

    Pasachoff, Jay M.; Olson, Roberta J. M.

    2014-06-01

    Howard Russell Butler (1856-1934) was invited to join the US Naval Observatory expedition to the total solar eclipse of 1918 because of his ability to paint astronomical phenomena based on quickly-made notes about spatial and color details. His giant triptych of the total eclipses of 1918, 1923, and 1925 was proposed for a never-built astronomical center at the American Museum of Natural History and wound up at their Hayden Planetarium when it was constructed in the mid-1930s. Half-size versions are installed at the Fels Planetarium at the Franklin Institute in Philadelphia and at the Firestone Library of Princeton University, whose newly conserved canvases were recently hung; the Buffalo Museum of Science has another half-size version in storage. We discuss not only the eclipse triptychs but also the series of large oil paintings he made of solar prominences (in storage at the American Museum of Natural History) and of his 1932-eclipse and other relevant works.JMP was supported for this work in part by Division III Discretionary Funds and the Brandi Fund of Williams College. His current eclipse research is supported by grants AGS-1047726 from the Solar Research Program of the Atmospheric and Geospace Sciences Division of NSF and 9327-13 from the Committee for Research and Exploration of the National Geographic Society.

  1. Biased inheritance of the protein PatN frees vegetative cells to initiate patterned heterocyst differentiation.

    PubMed

    Risser, Douglas D; Wong, Francis C Y; Meeks, John C

    2012-09-18

    Heterocysts, cells specialized for nitrogen fixation in certain filamentous cyanobacteria, appear singly in a nonrandom spacing pattern along the chain of vegetative cells. A two-stage, biased initiation and competitive resolution model has been proposed to explain the establishment of this spacing pattern. There is substantial evidence that competitive resolution of a subset of cells initiating differentiation occurs by interactions between a self-enhancing activator protein, HetR, and a diffusible pentapeptide inhibitor PatS-5 (RGSGR). Results presented here show that the absence of a unique membrane protein, PatN, in Nostoc punctiforme strain ATCC 29133 leads to a threefold increase in heterocyst frequency and a fourfold decrease in the vegetative cell interval between heterocysts. A PatN-GFP translational fusion shows a pattern of biased inheritance in daughter vegetative cells of ammonium-grown cultures. Inactivation of another heterocyst patterning gene, patA, is epistatic to inactivation of patN, and transcription of patA increases in a patN-deletion strain, implying that patN may function by modulating levels of patA. The presence of PatN is hypothesized to decrease the competency of a vegetative cell to initiate heterocyst differentiation, and the cellular concentration of PatN is dependent on cell division that results in cells transiently depleted of PatN. We suggest that biased inheritance of cell-fate determinants is a phylogenetic domain-spanning paradigm in the development of biological patterns.

  2. Pat Thiel talks about attending the Nobel Prize Award Ceremony

    SciTech Connect

    Thiel, Pat

    2012-01-01

    Pat Thiel, Ames Laboratory senior scientist and Iowa State University Distinguished Professor of Chemistry, was invited to be a guest at the ceremony on December 10th, in Stockholm, Sweden, where Danny Shechtman, Ames Laboratory scientist, received the 2011 Nobel Prize in Chemistry. Following her return to the Lab, Thiel shared some of her recollections of the momentous event.

  3. Stable phantoms for characterization of photoacoustic tomography (PAT) systems

    NASA Astrophysics Data System (ADS)

    Bohndiek, Sarah E.; Van de Sompel, Dominique; Bodapati, Sandhya; Kothapalli, Sri Rajasekhar; Gambhir, Sanjiv S.

    2013-02-01

    Photoacoustic tomography (PAT) is an emerging modality that combines the high contrast of optical imaging, with the spatial resolution and penetration depth of ultrasound, by exploiting the photoacoustic effect. As with any new imaging modality, reliable physical phantoms are needed to: calibrate instruments; validate performance; optimize signal-to-noise; perform routine quality control; and compare systems. Phantom materials for testing small animal PAT systems should also mimic both the optical and acoustic properties of soft tissue, while for calibration purposes should be resistant to degradation over long time periods. We show here that polyvinyl chloride plastisol (PVCP) phantoms enable calibration and performance validation using two PAT systems with distinct designs (Visualsonics Vevo LAZR and Endra Nexus 128) across a wavelength range of 680 nm - 950 nm. Inclusions between 2 and 3.2 mm in diameter were fabricated from PVCP using a range of dye concentrations (0 % to 0.256 % Black Plastic Color, BPC) in a custom mold. A calibration phantom was imaged repeatedly on both systems, over time scales of minutes, hours and days, to assess system stability. Both systems demonstrated good reproducibility over time, with the coefficient of variation in the measured signal-to-noise ratio (SNR) being less than 15% over the course of 30 days. Imaging performance was optimized by plotting SNR as a function of different system parameters. The visualization of objects embedded in optically absorbing and scattering backgrounds was also assessed. PVCP is easy to work with and provides stable phantoms for assessing PAT system performance.

  4. Stimulant-Related Reductions of Growth Rates in the PATS

    ERIC Educational Resources Information Center

    Swanson, James; Greenhill, Laurence; Wigal, Tim; Kollins, Scott; Stehli, Annamarie; Davies, Mark; Chuang, Shirley; Vitiello, Benedetto; Skrobala, Anne; Posner, Kelly; Abikoff, Howard; Oatis, Melvin; McCracken, James; McGough, James; Riddle, Mark; Ghuman, Jaswinder; Cunningham, Charles; Wigal, Sharon

    2006-01-01

    Objective: To investigate growth of children with attention-deficit/hyperactivity disorder (ADHD) in the Preschool ADHD Treatment Study (PATS) before and after initiation of treatment with methylphenidate at titrated doses (average, 14.2 mg/day) administered three times daily, 7 days/week for approximately equal to 1 year. Method: The heights and…

  5. Pat Mora, Fiction/Nonfiction Writer and Poet (Profile).

    ERIC Educational Resources Information Center

    Barrera, Rosalinda B.

    1998-01-01

    Offers a profile of Pat Mora, a writer whose work crosses the boundaries of audience, genre, and language. Notes that woven through her works are topics drawn from her life experience. Looks at her recent accomplishments and forthcoming books. Discusses her advocacy for cultural and linguistic diversity, and for more Latinos in all phases of…

  6. Pat Thiel talks about attending the Nobel Prize Award Ceremony

    ScienceCinema

    Thiel, Pat

    2016-07-12

    Pat Thiel, Ames Laboratory senior scientist and Iowa State University Distinguished Professor of Chemistry, was invited to be a guest at the ceremony on December 10th, in Stockholm, Sweden, where Danny Shechtman, Ames Laboratory scientist, received the 2011 Nobel Prize in Chemistry. Following her return to the Lab, Thiel shared some of her recollections of the momentous event.

  7. Project Achieve Transition Services (PATS), 1993-94. OER Report.

    ERIC Educational Resources Information Center

    Goldberg, Phyllis

    Project Achieve Transition Services (PATS) is a four-year high school attendance improvement, dropout prevention project which targets late-entry students consisting primarily of immigrants, transfers, and long-term absentees. The program uses a case management approach to provide support services, instructional enhancements, and family outreach…

  8. 10. GLASS, SCHNEIDER & REZNER BRIDGE PATENT MODEL, PAT. NO. ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    10. GLASS, SCHNEIDER & REZNER BRIDGE PATENT MODEL, PAT. NO. 71, 868, DECEMBER 10, 1867. THIS MODEL IS ONE OF A FEW THAT SURVIVED THE PATENT OFFICE FIRE OF 1877. IF REPRODUCED, CREDIT SHOULD BE GIVEN TO THE SMITHSONIAN INSTITUTION, NEGATIVE NO. 48660-D. - White Bowstring Arch Truss Bridge, Spanning Yellow Creek at Cemetery Drive (Riverside Drive), Poland, Mahoning County, OH

  9. Bacillus anthracis acetyltransferases PatA1 and PatA2 modify the secondary cell wall polysaccharide and affect the assembly of S-layer proteins.

    PubMed

    Lunderberg, J Mark; Nguyen-Mau, Sao-Mai; Richter, G Stefan; Wang, Ya-Ting; Dworkin, Jonathan; Missiakas, Dominique M; Schneewind, Olaf

    2013-03-01

    The envelope of Bacillus anthracis encompasses a proteinaceous S-layer with two S-layer proteins (Sap and EA1). Protein assembly in the envelope of B. anthracis requires S-layer homology domains (SLH) within S-layer proteins and S-layer-associated proteins (BSLs), which associate with the secondary cell wall polysaccharide (SCWP), an acetylated carbohydrate that is tethered to peptidoglycan. Here, we investigated the contributions of two putative acetyltransferases, PatA1 and PatA2, on SCWP acetylation and S-layer assembly. We show that mutations in patA1 and patA2 affect the chain lengths of B. anthracis vegetative forms and perturb the deposition of the BslO murein hydrolase at cell division septa. The patA1 and patA2 mutants are defective for the assembly of EA1 in the envelope but retain the ability of S-layer formation with Sap. SCWP isolated from the patA1 patA2 mutant lacked acetyl moieties identified in wild-type polysaccharide and failed to associate with the SLH domains of EA1. A model is discussed whereby patA1- and patA2-mediated acetylation of SCWP enables the deposition of EA1 as well as BslO near the septal region of the B. anthracis envelope.

  10. Rapid Response to the Howard Hanson Dam Crisis

    NASA Astrophysics Data System (ADS)

    Ralph, F. M.; Carter, G.; White, A.; Neiman, P. J.; King, C.; Jankov, I.; Colman, B.; Cook, K.; Buehner, T.

    2010-12-01

    Dedicated in 1962, the Howard A. Hanson Dam (HHD) brought necessary flood relief to the Green River Valley in the Metropolitan Area of Seattle, Washington, and opened the way for increased valley development. For example, the flood damage prevented by HHD during the extreme precipitation event in early January 2009 is estimated to be about $4 billion. However, following the record high level of water behind HHD caused by this event, the U.S. Army Corps of Engineers (ACE) became concerned about the Dam’s safety. Despite short-term measures to improve HHD during 2009, the chance for a significant Green River Valley flood event was estimated by the ACE to be 1 in 25 for the 2009/10 winter season. In response to this elevated risk, NOAA organized a coordinated effort across research and forecast operations to implement new observations, modeling and dissemination tools, and knowledge of the role of atmospheric rivers (ARs) in producing extreme precipitation, prototyped in California within NOAA’s Hydrometeorology Testbed (HMT; hmt.noaa.gov). Fortunately, although seasonal observed precipitation (October 2009 through March 2010) in the Seattle area was slightly greater than normal (e.g., 112% of normal at the Seattle National Weather Service Forecast Office), there were no threatening floods observed along the Green River. This outcome was influenced by a synoptic pattern that was progressive, i.e., storms did not stall over this vulnerable watershed, which reduced the chance for an extensive (time and space) extreme event. More than a decade of West Coast winter storm research conducted primarily in California by NOAA’s Earth System Research Laboratory/Physical Sciences Division (ESRL/PSD) has identified atmospheric rivers (ARs), narrow regions of enhanced water vapor transport, as the culprits that cause extreme precipitation events, such as the January 2009 event that stressed HHD. ESRL/PSD extended this AR research to the coast of Washington by deploying a

  11. Inverse transport problems in quantitative PAT for molecular imaging

    NASA Astrophysics Data System (ADS)

    Ren, Kui; Zhang, Rongting; Zhong, Yimin

    2015-12-01

    Fluorescence photoacoustic tomography (fPAT) is a molecular imaging modality that combines photoacoustic tomography with fluorescence imaging to obtain high-resolution imaging of fluorescence distributions inside heterogeneous media. The objective of this work is to study inverse problems in the quantitative step of fPAT where we intend to reconstruct physical coefficients in a coupled system of radiative transport equations using internal data recovered from ultrasound measurements. We derive uniqueness and stability results on the inverse problems and develop some efficient algorithms for image reconstructions. Numerical simulations based on synthetic data are presented to validate the theoretical analysis. The results we present here complement these in Ren K and Zhao H (2013 SIAM J. Imaging Sci. 6 2024-49) on the same problem but in the diffusive regime.

  12. ATP analog-sensitive Pat1 protein kinase for synchronous fission yeast meiosis at physiological temperature

    PubMed Central

    Cipak, Lubos; Hyppa, Randy; Smith, Gerald; Gregan, Juraj

    2012-01-01

    To study meiosis, synchronous cultures are often indispensable, especially for physical analyses of DNA and proteins. A temperature-sensitive allele of the Pat1 protein kinase (pat1-114) has been widely used to induce synchronous meiosis in the fission yeast Schizosaccharomyces pombe, but pat1-114-induced meiosis differs from wild-type meiosis, and some of these abnormalities might be due to higher temperature needed to inactivate the Pat1 kinase. Here, we report an ATP analog-sensitive allele of Pat1 [Pat1(L95A), designated pat1-as2] that can be used to generate synchronous meiotic cultures at physiological temperature. In pat1-as2 meiosis, chromosomes segregate with higher fidelity, and spore viability is higher than in pat1-114 meiosis, although recombination is lower by a factor of 2–3 in these mutants than in starvation-induced pat1+ meiosis. Addition of the mat-Pc gene improved chromosome segregation and spore viability to nearly the level of starvation-induced meiosis. We conclude that pat1-as2 mat-Pc cells offer synchronous meiosis with most tested properties similar to those of wild-type meiosis. PMID:22487684

  13. Dividing the pie: differential dung pat size utilization by sympatric Haematobia irritans and Musca autumnalis.

    PubMed

    Fowler, F E; Mullens, B A

    2016-06-01

    Horn flies [Haematobia irritans (Diptera: Muscidae) (L.)] and face flies [Musca autumnalis (Diptera: Muscidae) De Geer] use the same larval resource, but their interactions are poorly studied. Dung pats (n = 350) were core sampled in the summers of 2012 and 2013 from irrigated pastures in Pomona, California, U.S.A. (34°03'N, 117°48'W) and held for face fly and horn fly emergence. Surface areas and estimated weights were recorded for each whole pat. Almost half (42.0%) of the pat cores yielded neither fly, 29.7% yielded horn flies only, 12.9% yielded face flies only and 15.4% yielded both flies. Of the fly-positive pats, surface area and mass were larger for face fly-occupied pats, whereas horn fly-occupied pats were smaller. Pats shared by the two species were intermediate. Horn flies per positive core were unaffected by the absence/presence of face flies, but half as many face flies emerged when pats were co-inhabited by horn flies. Face flies inhabited larger pats, which might better resist heating and drying, to which they are susceptible; horn flies inhabited a broad pat size range. Horn fly tolerance of lower dung moisture probably allows horn flies to colonize and survive in a wide range of pats in dry areas like southern California.

  14. Flood-Inundation Maps for a 1.6-Mile Reach of Salt Creek, Wood Dale, Illinois

    USGS Publications Warehouse

    Soong, David T.; Murphy, Elizabeth A.; Sharpe, Jennifer B.

    2012-01-01

    Digital flood-inundation maps for a 1.6-mile reach of Salt Creek from upstream of the Chicago, Milwaukee, St. Paul & Pacific Railroad to Elizabeth Drive, Wood Dale, Illinois, were created by the U.S. Geological Survey (USGS) in cooperation with the DuPage County Stormwater Management Division. The inundation maps, which can be accessed through the USGS Flood Inundation Mapping Science Web site at http://water.usgs.gov/osw/flood_inundation/ depict estimates of the areal extent of flooding corresponding to selected water levels (gage heights) at the USGS streamgage on Salt Creek at Wood Dale, Illinois (station number 05531175). Current conditions at the USGS streamgage may be obtained on the Internet at http://waterdata.usgs.gov/usa/nwis/uv?05531175. In this study, flood profiles were computed for the stream reach by means of a one-dimensional unsteady flow Full EQuations (FEQ) model. The unsteady flow model was verified by comparing the rating curve output for a September 2008 flood event to discharge measurements collected at the Salt Creek at Wood Dale gage. The hydraulic model was then used to determine 14 water-surface profiles for gage heights at 0.5-ft intervals referenced to the streamgage datum and ranging from less than bankfull to approximately the highest recorded water level at the streamgage. The simulated water-surface profiles were then combined with a Geographic Information System (GIS) Digital Elevation Model (DEM) (derived from Light Detection and Ranging (LiDAR) data) in order to delineate the area flooded at each water level. The areal extent of the inundation was verified with high-water marks from a flood in July 2010 with a peak gage height of 14.08 ft recorded at the Salt Creek at Wood Dale gage. The availability of these maps along with Internet information regarding current gage height from USGS streamgages provide emergency management personnel and residents with information that is critical for flood response activities such as

  15. Myxomatosis: the introduction of the European rabbit flea Spilopsyllus cuniculi (Dale) into wild rabbit populations in Australia.

    PubMed

    Sobey, W R; Conolly, D

    1971-09-01

    1. The European rabbit flea Spilopsyllus cuniculi (Dale) bred successfully in wild rabbits on three properties in New South Wales and, within two breeding seasons, almost every rabbit shot within a quarter of a mile of a release site was infested.2. It was demonstrated that the flea transmitted myxoma virus in the field.3. In areas where more than 75% of the rabbits shot at the beginning of the breeding season were flea-infested and myxoma virus was present, populations failed to show the expected summer build-up.

  16. Overview of the High Performance Antiproton (HiPAT) Experiment

    NASA Technical Reports Server (NTRS)

    Martin, James J.; Sims, William H.; Chakrabarti, Suman; Pearson, Boise; Fant, Wallace E.; Lewis, Raymond A.; Rodgers, Stephen (Technical Monitor)

    2002-01-01

    The annihilation of matter with antimatter represents the highest energy density of any known reaction, producing 10(exp 8) MJ/g, approximately 10 orders of magnitude more energy per unit mass than chemical based combustion. To take the first step towards using this energy for propulsion applications the NASA MSFC Propulsion Research Center (PRC) has initiated a research activity examining the storage of low energy antiprotons. Storage was identified as a key enabling technology since it builds the experience base necessary to understand the handling of antiprotons for virtually all utilization and high-density storage concepts. To address this need, a device referred to as the High Performance Antiproton Trap (HiPAT) is under development at the NASA MSFC PRC. The HiPAT is an electromagnetic system (Penning-Malmberg design) consisting of a 4 Tesla superconductor, a high voltage confinement electrode system (operation up to 20 KV), and an ultra high vacuum test section (operating in the 10(exp -12) torr range). The system was designed to be portable with an ultimate goal of maintaining 10(exp 12) charged particles with a half-life of 18 days. Currently, this system is being experimentally evaluated using normal matter ions which are cheap to produce and relatively easy to handle. These normal ions provide a good indication of overall trap behavior, with the exception of assessing annihilation losses. The ions are produced external to HiPAT using two hydrogen ion sources, with adjustable beam energy and current. Ion are transported in a beam line and controlled through the use of electrostatic optics. These optics serve to both focus and gate the incoming ions, providing microsecond-timed pulses that are dynamically captured by cycling the HiPAT electric containment field like a 'trap door'. The layout of this system more closely simulates the operations expected at an actual antiproton production facility where 'packets' of antiprotons with pulse widths measured in

  17. Involvement of the Putative ATP-Dependent Efflux Proteins PatA and PatB in Fluoroquinolone Resistance of a Multidrug-Resistant Mutant of Streptococcus pneumoniae

    PubMed Central

    Marrer, Estelle; Schad, Karen; Satoh, Andreas T.; Page, Malcolm G. P.; Johnson, Maggie M.; Piddock, Laura J. V.

    2006-01-01

    The multidrug-resistant mutant Streptococcus pneumoniae M22 constitutively overexpresses two genes (patA and patB) that encode proteins homologous to known efflux proteins belonging to the ABC transporter family. It is shown here that PatA and PatB were strongly induced by quinolone antibiotics and distamycin in fluoroquinolone-sensitive strains. PatA was very important for growth of S. pneumoniae, and it could not be disrupted in strain M22. PatB appeared to control metabolic activity, particularly in amino acid biosynthesis, and it may have a pivotal role in coordination of the response to quinolone antibiotics. The induction of PatA and PatB by antibiotics showed a pattern similar to that exhibited by SP1861, a homologue of ABC-type transporters of choline and other osmoprotectants. A second group of quinolone-induced transporter genes comprising SP1587 and SP0287, which are homologues of, respectively, oxalate/formate antiporters and xanthine or uracil permeases belonging to the major facilitator family, showed a different pattern of induction by other antibiotics. There was no evidence for the involvement of PmrA, the putative proton-dependent multidrug transporter that has been implicated in norfloxacin resistance, in the response to quinolone antibiotics in either the resistant mutant or the fluoroquinolone-sensitive strains. PMID:16436727

  18. Construction and operation of the Howard T. Ricketts Laboratory.

    SciTech Connect

    Van Lonkhuyzen, R.; Stull, L.; Butler, J.; Chang, Y.; Allison, T.; O'Rourke, D.

    2006-01-01

    The National Institutes of Health (NIH) has proposed to partially fund the construction of the Howard T. Ricketts (HTR) regional biocontainment laboratory (RBL) by the University of Chicago at the U.S. Department of Energy's (DOE's) Argonne National Laboratory in Argonne, Illinois. The HTR Laboratory (HTRL) would be constructed, owned, and operated by the University of Chicago on land leased to it by DOE. The preferred project site is located north of Eastwood Drive and west of Outer Circle Road and is near the biological sciences building. This environmental assessment addresses the potential environmental effects resulting from construction and operation of the proposed facility. The proposed project involves the construction of a research facility with a footprint up to approximately 44,000 ft{sup 2} (4,088 m{sup 2}). The proposed building would house research laboratories, including Biosafety Level 2 and 3 biocontainment space, animal research facilities, administrative offices, and building support areas. The NIH has identified a need for new facilities to support research on potential bioterrorism agents and emerging and re-emerging infectious diseases, to protect the nation from such threats to public health. This research requires specialized laboratory facilities that are designed, managed, and operated to protect laboratory workers and the surrounding community from accidental exposure to agents. The proposed HTRL would provide needed biocontainment space to researchers and promote the advancement of knowledge in the disciplines of biodefense and emerging and re-emerging infectious diseases. Several alternatives were considered for the location of the proposed facility, as well as a no action alternative. The preferred alternative includes the construction of a research facility, up to 44,000 ft{sup 2} (4,088 m{sup 2}), at Argonne National Laboratory, a secure government location. Potential impacts to natural and cultural resources have been evaluated in

  19. Perfluoroalkyl substances in the Daling River with concentrated fluorine industries in China: seasonal variation, mass flow, and risk assessment.

    PubMed

    Zhu, Zhaoyun; Wang, Tieyu; Meng, Jing; Wang, Pei; Li, Qifeng; Lu, Yonglong

    2015-07-01

    In the present study, we investigated concentrations and composition profiles of perfluoroalkyl substances (PFASs) in surface water from the Daling River in different seasons. The highest concentration of ΣPFASs (9540 ng L(-1)) and dominant homologues were found in surface water collected in summer. Perfluorobutanoic acid (PFBA), perfluorobutane sulfonate (PFBS), and perfluorooctanoic acid (PFOA) were the dominant PFASs in four seasons with a total contribution of over 90%. The discharge of two fluorine chemical industry parks was predicted to be the main contamination source of PFASs in the study area. The daily and annual mass flows were calculated according to data of detected PFAS concentrations and water discharge, and the annual mass loading of PFASs into the Bohai Sea from the Daling River reached to 461 kg year(-1). Hazard assessment indicated that concentrations of PFASs in study area had little risk to aquatic organisms and wildlife. However, the multiple health risk would be relatively high if the water was served as drinking water in the study area.

  20. The cytoplasmic mRNA degradation factor Pat1 is required for rRNA processing

    PubMed Central

    Muppavarapu, Mridula; Huch, Susanne; Nissan, Tracy

    2016-01-01

    ABSTRACT Pat1 is a key cytoplasmic mRNA degradation factor, the loss of which severely increases mRNA half-lives. Several recent studies have shown that Pat1 can enter the nucleus and can shuttle between the nucleus and the cytoplasm. As a result, many nuclear roles have been proposed for Pat1. In this study, we analyzed four previously suggested nuclear roles of Pat1 and show that Pat1 is not required for efficient pre-mRNA splicing or pre-mRNA decay in yeast. However, lack of Pat1 results in accumulation of pre-rRNA processing intermediates. Intriguingly, we identified a novel genetic relationship between Pat1 and the rRNA decay machinery, specifically the exosome and the TRAMP complex. While the pre-rRNA processing intermediates that accumulate in the pat1 deletion mutant are, at least to some extent, recognized as aberrant by the rRNA degradation machinery, it is unlikely that these accumulations are the cause of their synthetic sick relationship. Here, we show that the dysregulation of the levels of mRNAs related to ribosome biogenesis could be the cause of the accumulation of the pre-rRNA processing intermediates. Although our results support a role for Pat1 in transcription, they nevertheless suggest that the primary cause of the dysregulated mRNA levels is most likely due to Pat1's role in mRNA decapping and mRNA degradation. PMID:26918764

  1. 77 FR 72349 - SBI International, Inc. v. Mr. Howard Finkel c/o Cosco Container Lines; Notice of Filing of...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-12-05

    ... SBI International, Inc. v. Mr. Howard Finkel c/o Cosco Container Lines; Notice of Filing of Complaint...,'' against Mr. Howard Finkel c/o Cosco Container Lines, hereinafter ``Respondent.'' Complainant alleges that ``4 refrigerated containers originating from the USA port of Wilmington, NC consisting of USA...

  2. 78 FR 35659 - Norfolk Southern Railway Company-Discontinuance of Service Exemption-in Tipton and Howard...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-06-13

    ... Tipton and Howard Counties, Ind. Norfolk Southern Railway Company (NSR) filed a verified notice of...), in Tipton and Howard Counties, Ind. The line traverses United States Postal Service Zip Codes 46068..., 2013. By the Board, Rachel D. Campbell, Director, Office of Proceedings. Derrick A. Gardner,...

  3. Unpacking Pat Parker: Intersections and Revolutions in "Movement in Black".

    PubMed

    Washburn, Amy

    2015-01-01

    This article explores Pat Parker's poem "Movement in Black." It examines the ways in which she emblematizes intersectionality and simultaneity as forms of revolution in struggles of self and society. It begins with a theoretical and historical apparatus to contextualize Parker as an artist and activist. Then it offers a literary analysis of the poem, focusing on themes of time and space, marginalization and movement, difference and power, visibility and invisibility, and history and memory. It argues that Parker uses autobiographical writing to fuse personal and political sites of resistance.

  4. Process analytical technology (PAT) for biopharmaceutical products: Part II. Concepts and applications.

    PubMed

    Read, E K; Shah, R B; Riley, B S; Park, J T; Brorson, K A; Rathore, A S

    2010-02-01

    Implementing real-time product quality control meets one or both of the key goals outlined in FDA's PAT guidance: "variability is managed by the process" and "product quality attributes can be accurately and reliably predicted over the design space established for materials used, process parameters, manufacturing, environmental, and other conditions." The first part of the paper presented an overview of PAT concepts and applications in the areas of upstream and downstream processing. In this second part, we present principles and case studies to illustrate implementation of PAT for drug product manufacturing, rapid microbiology, and chemometrics. We further present our thoughts on how PAT will be applied to biotech processes going forward. The role of PAT as an enabling component of the Quality by Design framework is highlighted. Integration of PAT with the principles stated in the ICH Q8, Q9, and Q10 guidance documents is also discussed.

  5. 75 FR 44936 - Ochoco National Forest, Lookout Mountain Ranger District; Oregon; Howard Elliot Johnson Fuels and...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-07-30

    ... [Federal Register Volume 75, Number 146 (Friday, July 30, 2010)] [Notices] [Page 44936] [FR Doc No: C1-2010-17803] DEPARTMENT OF AGRICULTURE Forest Service Ochoco National Forest, Lookout Mountain Ranger District; Oregon; Howard Elliot Johnson Fuels and Vegetation Management Project EIS Correction...

  6. New Directions for Higher Education: Q&A with Muriel Howard on Public Higher Ed

    ERIC Educational Resources Information Center

    DiSalvio, Philip

    2014-01-01

    Nearly a year ago, "NEJHE" launched its "New Directions for Higher Education" series to examine emerging issues, trends, and ideas that have an impact on higher education policies, programs, and practices. In this installment, DiSalvio interviews Muriel A. Howard, president of the American Association of State Colleges and…

  7. Black, White, Greek...Like Who?: Howard University Student Perceptions of a White Fraternity on Campus

    ERIC Educational Resources Information Center

    Hughey, Matthew W.

    2006-01-01

    On March 2, 1867, the Historically Black College or University (HBCU) Howard University (HU) was founded in Washington, D. C. Almost exactly one year later, the all white fraternity of Pi Kappa Alpha was founded at the University of Virginia. Over 100 years later, on February 18 2006, fifty-five HU students became charter members of Pi Kappa Alpha…

  8. Commission on the Future of Howard Community College Progress Report, 2001.

    ERIC Educational Resources Information Center

    Howard Community Coll., Columbia, MD. Office of Planning and Evaluation.

    This Howard Community College (HCC) (Maryland) 2001 Progress Report makes assessments and recommendations in the following areas: (1) creating a world class learning organization; (2) collaboration with other educational organizations; (3) collaboration with business and industry; (4) economics and workforce development; (5) preparing students as…

  9. Team Teaching Verbal, Mathematics, and Learning Skills. Howard University. The Center for Academic Reinforcement.

    ERIC Educational Resources Information Center

    Bartlett, Joan; Byrd, Roland

    Team teaching was used in three undergraduate courses to explore its potential for enhancing students' academic development. The courses were part of a program offered to freshmen with unrealized academic potential through the Howard University (District of Columbia) Center for Academic Reinforcement (CAR). A three-hour block of time was set aside…

  10. Howard Gardner: Knowledge, Learning and Development in Drama and Arts Education.

    ERIC Educational Resources Information Center

    Roper, Bill; Davis, David

    2000-01-01

    Notes that Howard Gardner's theory of Multiple Intelligences (MI) has afforded support to educators and parents who want to see their children as equals no matter in what area of ability they develop competence. Explains the usefulness of Gardner's approach, based on Kantian notions of the power of mind to give order to the world. Discusses the…

  11. Principles and Practices Fostering Inclusive Excellence: Lessons from the Howard Hughes Medical Institute's Capstone Institutions

    ERIC Educational Resources Information Center

    DiBartolo, Patricia Marten; Gregg-Jolly, Leslie; Gross, Deborah; Manduca, Cathryn A.; Iverson, Ellen; Cooke, David B., III; Davis, Gregory K.; Davidson, Cameron; Hertz, Paul E.; Hibbard, Lisa; Ireland, Shubha K.; Mader, Catherine; Pai, Aditi; Raps, Shirley; Siwicki, Kathleen; Swartz, Jim E.

    2016-01-01

    Best-practices pedagogy in science, technology, engineering, and mathematics (STEM) aims for inclusive excellence that fosters student persistence. This paper describes principles of inclusivity across 11 primarily undergraduate institutions designated as Capstone Awardees in Howard Hughes Medical Institute's (HHMI) 2012 competition. The Capstones…

  12. Written Assignments for Abnormal Psychology at Howard Community College, Fall 1988.

    ERIC Educational Resources Information Center

    Bell, James

    Designed for students enrolled in an Abnormal Psychology course at Howard Community College (Maryland), this booklet explains the requirements for the course's writing assignments, which are designed to teach the skills of comparison and contrast, analysis, critical thinking, and synthesis. Following an overview of class assignments and…

  13. The Evolution of Creativity, Giftedness, and Multiple Intelligences: An Interview with Ellen Winner and Howard Gardner

    ERIC Educational Resources Information Center

    Henshon, Suzanna E.

    2006-01-01

    This article presents an interview with Ellen Winner and Howard Gardner. Winner is Professor of Psychology at Boston College, and Senior Research Associate at Project Zero, Harvard Graduate School of Education. Her research focuses on learning and cognition in the arts in typical and gifted children. She is the author of over 100 articles and…

  14. Project Probes Digital Media's Effect on Ethics: Howard Gardner Leads Team Studying Youths' Web Norms

    ERIC Educational Resources Information Center

    Viadero, Debra

    2008-01-01

    Noted Harvard psychologist Howard Gardner is leading a team studying the social and ethical norms of young people on the Web. Known as the "GoodPlay Project," the study is being financed with a grant from the Chicago-based Catherine T. MacArthur Foundation. What researchers hope to do through the project is fill a gap in the burgeoning research…

  15. Howard Zinn and the Struggle for Real History in the United States

    ERIC Educational Resources Information Center

    Whitson, James Anthony

    2009-01-01

    Criticisms of Howard Zinn's work on U.S. History are examined in the context of increasing emphasis on the idea of "American exceptionalism" in contemporary political and cultural discourse, and particularly the insertion of American exceptionalism into revised social studies standards for the state of Texas. Analysis focusing on the…

  16. 75 FR 18826 - Mr. Howard Rosenfeld; Notice of Preliminary Permit Application Accepted for Filing and Soliciting...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-04-13

    ... kilowatt-hours (kWh), which would be used by the applicant on-site or distributed to the power grid and... 22, 2010, Mr. Howard Rosenfeld filed an application, pursuant to section 4(f) of the Federal Power... distribution line owned by Connecticut Power & Light and adjacent to the applicant's property; and...

  17. Revitalizing Politics Now and Then: Howard Zinn on Dissent, Democracy, and Education

    ERIC Educational Resources Information Center

    Cooley, Aaron

    2009-01-01

    This paper presents a discussion of Howard Zinn's intellectual and political ideas. Through the analysis of selections from his immense body of work, several interrelated themes emerge. Drawing more attention to these notions of dissent and democracy is crucial to revitalizing education at all levels and vital to advancing the public discourse…

  18. Development of the Right of Publicity Since Zacchini v. Scripps-Howard.

    ERIC Educational Resources Information Center

    List, Karen K.

    This paper deals with the development of the right of publicity since "Zacchini v Scripps-Howard," a case in which the Supreme Court ruled in favor of Hugo Zacchini, a "human cannonball" who had charged that his professional property had been unlawfully appropriated by a television station that had filmed and shown his performance without…

  19. Critical Uses of History: A Memory of Howard Zinn and Video Journalism

    ERIC Educational Resources Information Center

    Price, Todd Alan

    2009-01-01

    In the following article I reflect on a few of the lessons provided by the late historian Howard Zinn, both his close reading of history, but even more so his critical insight "that history is made, not only received". Zinn used history much like a muckraking joumalist would: he wrote history not only "through" the eyes of common people, but "for"…

  20. Growing into Public Service: William Howard Taft's Boyhood Home. Teaching with Historic Places.

    ERIC Educational Resources Information Center

    Henderson, E. Ray

    1994-01-01

    Presents a lesson plan based on primary sources about the home in which William Howard Taft was born and grew up. Provides student objectives, instructional procedures, and suggested student activities. Includes two documents, two map exhibits, three student readings, and two photographs. (CFR)

  1. The Pragmatic Idealist: Valerie Gross--Howard County Library, Columbia, MD

    ERIC Educational Resources Information Center

    Library Journal, 2004

    2004-01-01

    In the two years since Valerie Gross became director of the Howard County Library (HCL), it has won the county's Community Organization of the Year award, its Accessibility Award, and its Chamber of Commerce's Non-Profit Business of the Year ACE award for contributions to education, economic development, and quality of life. And for the first…

  2. Thermodynamics and Chemistry by Howard DeVoe

    NASA Astrophysics Data System (ADS)

    Gislason, Eric A.

    2001-09-01

    Prentice Hall: Upper Saddle River, NJ, 2001. 439 pp. ISBN 0-02-328741-1. $90.00. Thermodynamics is a deceptively difficult subject that few people master in their first exposure (typically in the junior-level physical chemistry course). Because of this, a clear, well-written textbook is always welcome to help students and teachers master this material. Such a book is Thermodynamics and Chemistry, by Howard DeVoe of the University of Maryland. This book is written as a one-semester textbook for senior undergraduates and graduate students who have had a previous course on the topic. This is a long book (400 pages of text, 25 pages of appendices), and it is unlikely that an instructor can cover everything in one semester. On the other hand this length does allow the author to cover topics such as a "liquid solution in a centrifugal field" that shorter books would omit. All important topics in chemical thermodynamics are covered. After two introductory chapters, the three laws of thermodynamics are taken up in Chapters 3 and 4. Pure substances, including phase transitions, are treated in Chapters 5 and 6. Chapter 7, "Mixtures," is a long chapter that covers, among other topics, partial molar quantities, activities, and activity coefficients. Chemical reactions are treated in Chapter 8 and there is a detailed discussion of equilibrium in various multicomponent systems in Chapter 9. The book is completed with a discussion of the phase rule (Chapter 10) and a brief chapter on galvanic cells. There are a number of things I like about this book. First, DeVoe takes great care in defining important thermodynamic words such as the thermodynamic state of a system. Similarly, he makes the distinction between process and path understandable, and this allows him to clearly define a reversible process as well as spontaneous and impossible processes. Section 4.1 then contains the sentence "An irreversible process is a spontaneous process whose reverse is an impossible process." This

  3. The Efflux Pump Inhibitor Reserpine Selects Multidrug-Resistant Streptococcus pneumoniae Strains That Overexpress the ABC Transporters PatA and PatB▿ †

    PubMed Central

    Garvey, Mark I.; Piddock, Laura J. V.

    2008-01-01

    One way to combat multidrug-resistant microorganisms is the use of efflux pump inhibitors (EPIs). Spontaneous mutants resistant to the EPI reserpine selected from Streptococcus pneumoniae NCTC 7465 and R6 at a frequency suggestive of a single mutational event were also multidrug resistant. No mutations in pmrA (which encodes the efflux protein PmrA) were detected, and the expression of pmrA was unaltered in all mutants. In the reserpine-resistant multidrug-resistant mutants, the overexpression of both patA and patB, which encode ABC transporters, was associated with accumulation of low concentrations of antibiotics and dyes. The addition of sodium orthovanadate, an inhibitor of ABC efflux pumps, or the insertional inactivation of either gene restored wild-type antibiotic susceptibility and wild-type levels of accumulation. Only when patA was insertionally inactivated were both multidrug resistance and reserpine resistance lost. Strains in which patA was insertionally inactivated grew significantly more slowly than the wild type. These data indicate that the overexpression of both patA and patB confers multidrug resistance in S. pneumoniae but that only patA is involved in reserpine resistance. The selection of reserpine-resistant multidrug-resistant pneumococci has implications for analogous systems in other bacteria or in cancer. PMID:18362193

  4. Dale Reed with X-38 and a Subscale Model Used in Test Program

    NASA Technical Reports Server (NTRS)

    1997-01-01

    Dale Reed, a NASA engineer who worked on the original lifting-body research programs in the 1960s and 1970s, stands with a scale-model X-38 that was used in 1995 research flights, with a full-scale X-38 (80 percent of the size of a potential Crew Return Vehicle) behind him. The X-38 Crew Return Vehicle (CRV) research project is designed to develop the technology for a prototype emergency crew return vehicle, or lifeboat, for the International Space Station. The project is also intended to develop a crew return vehicle design that could be modified for other uses, such as a joint U.S. and international human spacecraft that could be launched on the French Ariane-5 Booster. The X-38 project is using available technology and off-the-shelf equipment to significantly decrease development costs. Original estimates to develop a capsule-type crew return vehicle were estimated at more than $2 billion. X-38 project officials have estimated that development costs for the X-38 concept will be approximately one quarter of the original estimate. Off-the-shelf technology is not necessarily 'old' technology. Many of the technologies being used in the X-38 project have never before been applied to a human-flight spacecraft. For example, the X-38 flight computer is commercial equipment currently used in aircraft and the flight software operating system is a commercial system already in use in many aerospace applications. The video equipment for the X-38 is existing equipment, some of which has already flown on the space shuttle for previous NASA experiments. The X-38's primary navigational equipment, the Inertial Navigation System/Global Positioning System, is a unit already in use on Navy fighters. The X-38 electromechanical actuators come from previous joint NASA, U.S. Air Force, and U.S. Navy research and development projects. Finally, an existing special coating developed by NASA will be used on the X-38 thermal tiles to make them more durable than those used on the space

  5. Dale Reed with X-38 and a Subscale Model Used in Test Program

    NASA Technical Reports Server (NTRS)

    1997-01-01

    Dale Reed, a NASA engineer who worked on the original lifting-body research programs in the 1960s and 1970s, stands with a scale-model X-38 that was used in 1995 research flights, with a full-scale X-38 (80 percent of the size of a potential Crew Return Vehicle) behind him. The X-38 Crew Return Vehicle (CRV) research project is designed to develop the technology for a prototype emergency crew return vehicle, or lifeboat, for the International Space Station. The project is also intended to develop a crew return vehicle design that could be modified for other uses, such as a joint U.S. and international human spacecraft that could be launched on the French Ariane-5 Booster. The X-38 project is using available technology and off-the-shelf equipment to significantly decrease development costs. Original estimates to develop a capsule-type crew return vehicle were estimated at more than $2 billion. X-38 project officials have estimated that development costs for the X-38 concept will be approximately one quarter of the original estimate. Off-the-shelf technology is not necessarily 'old' technology. Many of the technologies being used in the X-38 project have never before been applied to a human-flight spacecraft. For example, the X-38 flight computer is commercial equipment currently used in aircraft and the flight software operating system is a commercial system already in use in many aerospace applications. The video equipment for the X-38 is existing equipment, some of which has already flown on the space shuttle for previous NASA experiments. The X-38's primary navigational equipment, the Inertial Navigation System/Global Positioning System, is a unit already in use on Navy fighters. The X-38 electromechanical actuators come from previous joint NASA, U.S. Air Force, and U.S. Navy research and development projects. Finally, an existing special coating developed by NASA will be used on the X-38 thermal tiles to make them more durable than those used on the space

  6. Rationale, Design, and Methods of the Preschool ADHD Treatment Study (PATS)

    ERIC Educational Resources Information Center

    Kollins, Scott; Greenhill, Laurence; Swanson, James; Wigal, Sharon; Abikoff, Howard; McCracken, James; Riddle, Mark; McGough, James; Vitiello, Benedetto; Wigal, Tim; Skrobala, Anne; Posner, Kelly; Ghuman, Jaswinder; Davies, Mark; Cunningham, Charles; Bauzo, Audrey

    2006-01-01

    Objective: To describe the rationale and design of the Preschool ADHD Treatment Study (PATS). Method: PATS was a National Institutes of Mental Health-funded, multicenter, randomized, efficacy trial designed to evaluate the short-term (5 weeks) efficacy and long-term (40 weeks) safety of methylphenidate (MPH) in preschoolers with…

  7. The Relationship between School Climate, PATS Program Participation, and Organizational Level.

    ERIC Educational Resources Information Center

    Kenney, Gordon E.; Butler, E. Dean

    In 1987, a school-university collaborative project, Positive Attitudes in Tennessee Schools (PATS), was established to improve school-learning environments. This paper presents findings of a study that investigated the effect of school participation in PATS on school climate. A secondary focus was to determine which school-climate variables could…

  8. The Preschool Attention-Deficit/Hyperactivity Disorder Treatment Study (PATS) 6-Year Follow-Up

    ERIC Educational Resources Information Center

    Riddle, Mark A.; Yershova, Kseniya; Lazzaretto, Deborah; Paykina, Natalya; Yenokyan, Gayane; Greenhill, Laurence; Abikoff, Howard; Vitiello, Benedetto; Wigal, Tim; McCracken, James T.; Kollins, Scott H.; Murray, Desiree W.; Wigal, Sharon; Kastelic, Elizabeth; McGough, James J.; dosReis, Susan; Bauzo-Rosario, Audrey; Stehli, Annamarie; Posner, Kelly

    2013-01-01

    Objective: To describe the clinical course of attention-deficit/hyperactivity disorder (ADHD) symptom severity and diagnosis from ages 3 to 5 up to 9 to 12 years during a 6-year follow-up after the original Preschool ADHD Treatment Study (PATS). Method: A total of 207 participants (75% male) from the original PATS, assessed at baseline (mean age,…

  9. Short-Term Memory after All: Comment on Sederberg, Howard, and Kahana (2008)

    ERIC Educational Resources Information Center

    Usher, Marius; Davelaar, Eddy J.; Haarmann, Henk J.; Goshen-Gottstein, Yonatan

    2008-01-01

    P. B. Sederberg, M. W. Howard, and M. J. Kahana have proposed an updated version of the temporal-context model (TCM-A). In doing so, they accepted the challenge of developing a single-store model to account for the dissociations between short- and long-term recency effects that were reviewed by E. J. Davelaar, Y. Goshen-Gottstein, A. Ashkenazi, H.…

  10. Molecular cloning and characterization of oocyte-specific Pat1a in Rana rugosa frogs.

    PubMed

    Nakamura, Yoriko; Iwasaki, Takehiro; Umei, Yosuke; Saotome, Kazuhiro; Nakajima, Yukiko; Kitahara, Shoichi; Uno, Yoshinobu; Matsuda, Yoichi; Oike, Akira; Kodama, Maho; Nakamura, Masahisa

    2015-10-01

    The Pat1 gene is expressed in the immature oocytes of Xenopus, and is reportedly involved in regulating the translation of maternal mRNAs required for oocyte-maturation. However, it is still unknown when Pat1a first appears in the differentiating ovary of amphibians. To address this issue, we isolated the full-length Pat1a cDNA from the frog Rana rugosa and examined its expression in the differentiating ovary of this frog. Among eight different tissues examined, the Pat1a mRNA was detectable in only the ovary. When frozen sections from the ovaries of tadpoles at various stages of development were immunostained for Vasa-a germ cell-specific protein-and Pat1a, Vasa-immunopositive signals were observed in all of the germ cells, whereas Pat1a signals were confined to the growing oocytes (50-200 μm in diameter), and absent from small germ cells (<50 μm in diameter). Forty days after testosterone injection into tadpoles to induce female-to-male sex-reversal, Pat1a-immunoreactive oocytes had disappeared completely from the sex-reversed gonad, but Vasa-positive small germ cells persisted. Thus, Pat1a would be a good marker for identifying the sexual status of the sex-reversing gonad in amphibians. In addition, fluorescence in situ hybridization analysis showed Pat1a to have an autosomal locus, suggesting that Pat1a transcription is probably regulated by a tissue-specific transcription factor in R. rugosa.

  11. From rat pathophysiology to genomic medicine: an interview with Howard Jacob

    PubMed Central

    2016-01-01

    Howard Jacob is best known for pioneering genomic sequencing of a patient to solve a mysterious pediatric case in 2010. With roots in pharmacology and cardiovascular disease, however, his career has largely been dedicated to dissecting the physiology and genetics of the rat to help understand complex human diseases. Howard was Director of the Human and Molecular Genetics Center at the Medical College of Wisconsin for 16 years, during which time he applied a combination of approaches, including quantitative genetics, integrative physiology and next-generation sequencing, in rat models to shed light on cardiovascular, metabolic and renal disorders. He was a key contributor to the genomic toolbox for rat research, and generated the first targeted-knockout rat models using zinc-finger-nuclease technology. He also contributed to sequencing of the rat genome and establishment of the Rat Genome Database. In this interview, Howard provides his perspectives on the past, present and future of rat-based translational research and explains why, despite his many successes as the leader of a rat group, he recently made the transition to clinical genomics. PMID:27736743

  12. Acquisition of a Spinning Disk Confocal Microscope to Enhance Research and Teaching Capabilities in the STEM Fields at Howard University

    DTIC Science & Technology

    2015-05-13

    Teaching Capabilities in the STEM Fields at Howard University The views, opinions and/or findings contained in this report are those of the author(s) and...Report: Acquisition of a Spinning Disk Confocal Microscope to Enhance Research and Teaching Capabilities in the STEM Fields at Howard University...ETR-1 have been implicated in myotonic muscular dystrophy when mutated. Recently, ETR- 1 was identified in a large -scale C. elegans RNAi suppressor

  13. Structural insights into HetR−PatS interaction involved in cyanobacterial pattern formation

    PubMed Central

    Hu, Hai-Xi; Jiang, Yong-Liang; Zhao, Meng-Xi; Cai, Kun; Liu, Sanling; Wen, Bin; Lv, Pei; Zhang, Yonghui; Peng, Junhui; Zhong, Hui; Yu, Hong-Mei; Ren, Yan-Min; Zhang, Zhiyong; Tian, Changlin; Wu, Qingfa; Oliveberg, Mikael; Zhang, Cheng-Cai; Chen, Yuxing; Zhou, Cong-Zhao

    2015-01-01

    The one-dimensional pattern of heterocyst in the model cyanobacterium Anabaena sp. PCC 7120 is coordinated by the transcription factor HetR and PatS peptide. Here we report the complex structures of HetR binding to DNA, and its hood domain (HetRHood) binding to a PatS-derived hexapeptide (PatS6) at 2.80 and 2.10 Å, respectively. The intertwined HetR dimer possesses a couple of novel HTH motifs, each of which consists of two canonical α-helices in the DNA-binding domain and an auxiliary α-helix from the flap domain of the neighboring subunit. Two PatS6 peptides bind to the lateral clefts of HetRHood, and trigger significant conformational changes of the flap domain, resulting in dissociation of the auxiliary α-helix and eventually release of HetR from the DNA major grove. These findings provide the structural insights into a prokaryotic example of Turing model. PMID:26576507

  14. Issues in development, evaluation, and use of the NASA Preflight Adaptation Trainer (PAT)

    NASA Technical Reports Server (NTRS)

    Lane, Norman E.; Kennedy, Robert S.

    1988-01-01

    The Preflight Adaptation Trainer (PAT) is intended to reduce or alleviate space adaptation syndrome by providing opportunities for portions of that adaptation to occur under normal gravity conditions prior to space flight. Since the adaptation aspects of the PAT objectives involve modification not only of the behavior of the trainee, but also of sensiomotor skills which underly the behavioral generation, the defining of training objectives of the PAT utilizes four mechanisms: familiarization, demonstration, training and adaptation. These mechanisms serve as structural reference points for evaluation, drive the content and organization of the training procedures, and help to define the roles of the PAT instructors and operators. It was determined that three psychomotor properties are most critical for PAT evaluation: reliability; sensitivity; and relevance. It is cause for concern that the number of measures available to examine PAT effects exceed those that can be properly studied with the available sample sizes; special attention will be required in selection of the candidate measure set. The issues in PAT use and application within a training system context are addressed through linking the three training related mechanisms of familiarization, demonstration and training to the fourth mechanism, adaptation.

  15. Early development of PAT-SM6 for the treatment of melanoma.

    PubMed

    Hensel, Frank; Eckstein, Miriam; Rosenwald, Andreas; Brändlein, Stephanie

    2013-08-01

    Despite the recent development of novel therapies for patients with metastatic melanoma, this disease remains fatal in the majority of those who develop a relapse. Here, we report the preclinical and early clinical development of a novel IgM antibody PAT-SM6 that specifically binds to a cancer-specific isoform of glucose-regulated protein 78 (GRP78) and low-density lipoprotein. Finding a GRP78 cancer-specific form on the surface of cancer cells, but not normal cells in vivo, presents an opportunity for cancer-specific targeting. PAT-SM6 binding to the cell surface induces apoptosis in a variety of tumors, including melanoma. Recent studies show the specificity of PAT-SM6 binding to the surface of melanoma cells and primary tissue but not to normal tissue. They also confirm, for the first time, cell proliferation inhibition and apoptosis through classical apoptotic pathways as well as induction of lipid accumulation in melanoma cells. These in-vitro data are supported by positive in-vivo data using PAT-SM6 in a xenograft C8161 model. Furthermore, PAT-SM6 was well tolerated in pharmacokinetic/toxicology studies in monkeys. On the basis of these preclinical observations, a clinical study of PAT-SM6 was carried out in patients with 'in-transit' melanoma. Even with microdosing, histological analyses of tumor biopsies detected the presence of PAT-SM6 as well as apoptosis. Although there are many small molecules and monoclonal antibodies currently in clinical development for patients with melanoma, PAT-SM6 is the only therapeutic targeting the cancer-specific isoform of GRP78. These PAT-SM6 preclinical data and positive findings from the phase 1 safety study provide strong support for the further development of this novel antibody.

  16. Polyamine metabolism is altered in unpollinated parthenocarpic pat-2 tomato ovaries.

    PubMed

    Fos, Mariano; Proaño, Karina; Alabadí, David; Nuez, Fernando; Carbonell, Juan; García-Martínez, José L

    2003-01-01

    Facultative parthenocarpy induced by the recessive mutation pat-2 in tomato (Lycopersicon esculentum Mill.) depends on gibberellins (GAs) and is associated with changes in GA content in unpollinated ovaries. Polyamines (PAs) have also been proposed to play a role in early tomato fruit development. We therefore investigated whether PAs are able to induce parthenocarpy and whether the pat-2 mutation alters the content and metabolism of PAs in unpollinated ovaries. Application of putrescine, spermidine, and spermine to wild-type unpollinated tomato ovaries (cv Madrigal [MA/wt]) induced partial parthenocarpy. Parthenocarpic growth of MA/pat-2 (a parthenocarpic near-isogenic line to MA/wt) ovaries was negated by paclobutrazol (GA biosynthesis inhibitor), and this inhibition was counteracted by spermidine. Application of alpha-difluoromethyl-ornithine (-Orn) and/or alpha-difluoromethyl-arginine (-Arg), irreversible inhibitors of the putrescine biosynthesis enzymes Orn decarboxylase (ODC) and Arg decarboxylase, respectively, prevented growth of unpollinated MA/pat-2 ovaries. Alpha-difluoromethyl-Arg inhibition was counteracted by putrescine and GA(3), whereas that of alpha-difluoromethyl-Orn was counteracted by GA(3) but not by putrescine or spermidine. In unpollinated MA/pat-2 ovaries, the content of free spermine was significantly higher than in MA/wt ovaries. ODC activity was higher in pat-2 ovaries than in MA/wt. Transcript levels of genes encoding ODC and spermidine synthase were also higher in MA/pat-2. All together, these results strongly suggest that the parthenocarpic ability of pat-2 mutants depends on elevated PAs levels in unpollinated mutant ovaries, which correlate with an activation of the ODC pathway, probably as a consequence of elevated GA content in unpollinated pat-2 tomato ovaries.

  17. The UK-DALE dataset, domestic appliance-level electricity demand and whole-house demand from five UK homes

    NASA Astrophysics Data System (ADS)

    Kelly, Jack; Knottenbelt, William

    2015-03-01

    Many countries are rolling out smart electricity meters. These measure a home’s total power demand. However, research into consumer behaviour suggests that consumers are best able to improve their energy efficiency when provided with itemised, appliance-by-appliance consumption information. Energy disaggregation is a computational technique for estimating appliance-by-appliance energy consumption from a whole-house meter signal. To conduct research on disaggregation algorithms, researchers require data describing not just the aggregate demand per building but also the ‘ground truth’ demand of individual appliances. In this context, we present UK-DALE: an open-access dataset from the UK recording Domestic Appliance-Level Electricity at a sample rate of 16 kHz for the whole-house and at 1/6 Hz for individual appliances. This is the first open access UK dataset at this temporal resolution. We recorded from five houses, one of which was recorded for 655 days, the longest duration we are aware of for any energy dataset at this sample rate. We also describe the low-cost, open-source, wireless system we built for collecting our dataset.

  18. Profile and bioconcentration of minerals by King Bolete (Boletus edulis) from the Płocka Dale in Poland.

    PubMed

    Frankowska, Aneta; Ziółkowska, Joanna; Bielawski, Leszek; Falandysz, Jerzy

    2010-01-01

    This study aimed to provide basic data on the composition of metallic elements, including toxicologically important Cd and Hg, in popular and prized wild King Bolete mushrooms. We investigated the importance of soil substratum as a source of these metals. ICP-OES and CV-AAS were applied to determine the profile of Al, Ba, Ca, Cd, Cu, Fe, Hg, K, Mg, Mn, Na, Sr and Zn in caps and stipes of King Bolete mushroom and in the surface layer of soil (0-10 cm) from the Płocka Dale area of Poland. Hg, Cu, Cd, Zn, Mg and K exhibited bioconcentration factors (BCF) > 1. Specifically, Hg, Cu and Cd (mean BCFs for caps were 110, 19 and 16, respectively) were efficiently bioconcentrated by King Bolete, while other elements were bioexcluded (BCF < 1). Cadmium was present in the caps at mean levels of 5.5 ± 2.4 mg kg(-1) dry weight (dw) and mercury at levels of 4.9 ± 1.4 mg kg(-1) dw, both occurring at elevated concentrations in those King Bolete mushrooms surveyed.

  19. The UK-DALE dataset, domestic appliance-level electricity demand and whole-house demand from five UK homes

    PubMed Central

    Kelly, Jack; Knottenbelt, William

    2015-01-01

    Many countries are rolling out smart electricity meters. These measure a home’s total power demand. However, research into consumer behaviour suggests that consumers are best able to improve their energy efficiency when provided with itemised, appliance-by-appliance consumption information. Energy disaggregation is a computational technique for estimating appliance-by-appliance energy consumption from a whole-house meter signal. To conduct research on disaggregation algorithms, researchers require data describing not just the aggregate demand per building but also the ‘ground truth’ demand of individual appliances. In this context, we present UK-DALE: an open-access dataset from the UK recording Domestic Appliance-Level Electricity at a sample rate of 16 kHz for the whole-house and at 1/6 Hz for individual appliances. This is the first open access UK dataset at this temporal resolution. We recorded from five houses, one of which was recorded for 655 days, the longest duration we are aware of for any energy dataset at this sample rate. We also describe the low-cost, open-source, wireless system we built for collecting our dataset. PMID:25984347

  20. The UK-DALE dataset, domestic appliance-level electricity demand and whole-house demand from five UK homes.

    PubMed

    Kelly, Jack; Knottenbelt, William

    2015-01-01

    Many countries are rolling out smart electricity meters. These measure a home's total power demand. However, research into consumer behaviour suggests that consumers are best able to improve their energy efficiency when provided with itemised, appliance-by-appliance consumption information. Energy disaggregation is a computational technique for estimating appliance-by-appliance energy consumption from a whole-house meter signal. To conduct research on disaggregation algorithms, researchers require data describing not just the aggregate demand per building but also the 'ground truth' demand of individual appliances. In this context, we present UK-DALE: an open-access dataset from the UK recording Domestic Appliance-Level Electricity at a sample rate of 16 kHz for the whole-house and at 1/6 Hz for individual appliances. This is the first open access UK dataset at this temporal resolution. We recorded from five houses, one of which was recorded for 655 days, the longest duration we are aware of for any energy dataset at this sample rate. We also describe the low-cost, open-source, wireless system we built for collecting our dataset.

  1. Synchronized fission yeast meiosis using an ATP analog-sensitive Pat1 protein kinase

    PubMed Central

    Cipak, Lubos; Polakova, Silvia; Hyppa, Randy W.; Smith, Gerald R.; Gregan, Juraj

    2014-01-01

    Synchronous cultures are often indispensable for studying meiosis. Here, we present an optimized protocol for induction of synchronous meiosis in the fission yeast Schizosaccharomyces pombe. Chemical inactivation of an ATP analog-sensitive form of the Pat1 kinase (pat1-as2) by adding the ATP-analog 1-NM-PP1 in G1-arrested cells allows induction of synchronous meiosis at optimal temperature (25 °C). Importantly, this protocol eliminates detrimental effects of elevated temperature (34 °C) which is required to inactivate the commonly used temperature-sensitive Pat1 kinase mutant (pat1-114). Addition of the mat-Pc gene to a mat1-M strain further improves chromosome segregation and spore viability. Thus, our protocol offers highly synchronous meiosis at optimal temperature with most characteristics similar to those of wild-type meiosis. The synchronization protocol can be completed in 5 days. PMID:24385151

  2. Air transport of plutonium metal: content expansion initiative for the plutonium air transportable (PAT01) packaging

    SciTech Connect

    Caviness, Michael L; Mann, Paul T

    2010-01-01

    The National Nuclear Security Administration (NNSA) has submitted an application to the Nuclear Regulatory Commission (NRC) for the air shipment of plutonium metal within the Plutonium Air Transportable (PAT-1) packaging. The PAT-1 packaging is currently authorized for the air transport of plutonium oxide in solid form only. The INMM presentation will provide a limited overview of the scope of the plutonium metal initiative and provide a status of the NNSA application to the NRC.

  3. Howard Young Brings Light to the Serious Side of Science | Poster

    Cancer.gov

    You know what they say about all work and no play. And without a doubt, science requires constant hard work. But the NCI at Frederick has an antidote to the serious side of science: Howard Young. Young, Ph.D., Senior Investigator, Cancer and Inflammation Program, is a serious scientist in his own right. He was part of the team that characterized and cloned the RAS oncogene, he developed a mouse model of lupus, and he was the first to show a clear sex bias, similar to that seen in patients, in what has been called the best mouse model for primary biliary cholangitis.

  4. HOWARD EISEN, JPL'S LEAD MECHANICAL TECHNICIAN, HOLDS MARS PATHFINDER 'SOJOURNER' ROVER 1:1 SCALE DU

    NASA Technical Reports Server (NTRS)

    1996-01-01

    The Mars Pathfinder 'Sojourner' rover l:l scale duplicate test vehicle is held by Howard Eisen, its lead mechanical technician from the Jet Propulsion Laboratory, with Kennedy Space Center's Vehicle Assembly Building looming in the background. The launch of NASA's Mars Pathfinder spacecraft aboard a McDonnell Douglas Delta II rocket is scheduled for Monday, Dec. 2, at 2:09:11 a.m. EST. This is a single instantaneous target launch time without a second opportunity on that day. Liftoff will occur from Pad B at Launch Complex 17 on Cape Canaveral Air Station, Fla. There is a 24-day launch opportunity which extends through Dec. 31.

  5. The Louis Stokes Health Sciences Library: the Howard University move experience

    PubMed Central

    Bryant, Darcel A.

    2004-01-01

    The Louis Stokes Health Sciences Library attributes its successful move to early planning and preparation. Professional literature on the subject as well as consultation with other experienced library personnel also proved beneficial. Utilizing these resources, the committees devised a strategy that supported the library's mission to provide excellent and complete information services for the advancement of health sciences. This paper describes the Howard University Health Sciences Library move experience and offers practical advice for planning a library move. We hope that the information shared will assist other libraries facing a similar challenge. PMID:15098055

  6. The efficacy of Watch PAT in obstructive sleep apnea syndrome diagnosis.

    PubMed

    Körkuyu, Emine; Düzlü, Mehmet; Karamert, Recep; Tutar, Hakan; Yılmaz, Metin; Çiftçi, Bülent; Güven, Selma Fırat

    2015-01-01

    Polysomnography is currently considered as the gold standard for the diagnosis of obstructive sleep apnea syndrome (OSAS). But high expense and the backlog of the sleep centers have resulted in a search for an alternative method of diagnosis. The aim of this study is to assess the efficacy and reliability of Watch PAT as an alternate option in OSAS diagnosis. The patients have worn a Watch PAT(®) 200 device in the sleep laboratory during a standard polysomnography. The correlation in REM and Non-REM AHI scores, sleep periods and the mean O2 saturation percentage between Watch PAT and PSG sleep studies were assessed. There was a statistically significant very strong correlation between PSG and Watch PAT AHI scores (Spearman's rho = 0.802 p < 0.001). The mean recording time with PSG and Watch PAT was 463.06 ± 37.08 and 469.33 ± 72.81 min, respectively, and there was no statistically significant difference (p = 0.068). However, there was a statistically significant difference between two methods regarding the average sleep time and REM sleep period. No statistically significant difference was revealed in the mean O2 saturation percentage (p < 0.001). Watch PAT is an efficient device and is considered to be an adjunctive diagnostic method for PSG in diagnosis of OSAS.

  7. Enfermedad diarreica aguda por Escherichia coli patógenas en Colombia

    PubMed Central

    Gómez-Duarte, Oscar G.

    2014-01-01

    Resumen Las cepas de E. coli patógenas intestinales son causas importantes de la enfermedad diarreica aguda (EDA) en niños menores de 5 años en América Latina, África y Asia y están asociadas a alta mortalidad en niños en las comunidades más pobres de África y el Sudeste Asiático. Estudios sobre el papel de las E. coli patógenas intestinales en la EDA infantil en Colombia y otros países de América Latina son limitados debido a la carencia de ensayos para detección de estos patógenos en los laboratorios clínicos de centros de salud. Estudios recientes han reportado la detección de E. coli patógenas intestinales en Colombia, siendo la E. coli enterotoxigénica la cepa más frecuentemente asociada a diarrea en niños menores de 5 años. Otros patógenos detectados en estos pacientes incluyen las E. coli enteroagregativa, enteropatógena, productora de toxina Shiga, y de adherencia difusa. Con base en estudios que reportan la presencia de E. coli productora de toxina Shiga y E. coli enteroagregativa en carnes y vegetales en supermercados, se cree que productos alimentarios contaminados contribuyen a la transmisión de estos patógenos y a la infección del huésped susceptible. Más estudios son necesarios para evaluar los mecanismos de transmisión, el impacto en la epidemiologia de la EDA, y las pautas de manejo y prevención de estos patógenos que afectan la población pediátrica en Colombia. PMID:25491457

  8. The gene pat-2, which induces natural parthenocarpy, alters the gibberellin content in unpollinated tomato ovaries.

    PubMed

    Fos, M; Nuez, F; García-Martínez, J L

    2000-02-01

    We investigated the role of gibberellins (GAs) in the effect of pat-2, a recessive mutation that induces facultative parthenocarpic fruit development in tomato (Lycopersicon esculentum Mill.) using near-isogenic lines with two different genetic backgrounds. Unpollinated wild-type Madrigal (MA/wt) and Cuarenteno (CU/wt) ovaries degenerated, but GA(3) application induced parthenocarpic fruit growth. On the contrary, parthenocarpic growth of MA/pat-2 and CU/pat-2 fruits, which occurs in the absence of pollination and hormone application, was not affected by GA(3). Pollinated MA/wt and parthenocarpic MA/pat-2 ovary development was negated by paclobutrazol, and this inhibitory effect was counteracted by GA(3). The main GAs of the early-13-hydroxylation pathway (GA(1), GA(3), GA(8), GA(19), GA(20), GA(29), GA(44), GA(53), and, tentatively, GA(81)) and two GAs of the non-13-hydroxylation pathway (GA(9) and GA(34)) were identified in MA/wt ovaries by gas chromatography-selected ion monitoring. GAs were quantified in unpollinated ovaries at flower bud, pre-anthesis, and anthesis. In unpollinated MA/pat-2 and CU/pat-2 ovaries, the GA(20) content was much higher (up to 160 times higher) and the GA(19) content was lower than in the corresponding non-parthenocarpic ovaries. The application of an inhibitor of 2-oxoglutarate-dependent dioxygenases suggested that GA(20) is not active per se. The pat-2 mutation may increase GA 20-oxidase activity in unpollinated ovaries, leading to a higher synthesis of GA(20), the precursor of an active GA.

  9. Clinical Usefulness of Watch-PAT for Assessing the Surgical Results of Obstructive Sleep Apnea Syndrome

    PubMed Central

    Park, Chong Yoon; Hong, Joon Hyeong; Lee, Jae Heon; Lee, Kyu Eun; Cho, Hyun Sang; Lim, Su Jin; Kwak, Jin Wook; Kim, Kyung Soo; Kim, Hyun Jik

    2014-01-01

    Objective: This study aimed to assess the accuracy and clinical efficacy of a wrist-worn device that is based on peripheral arterial tonometry (watch-PAT) to evaluate the surgical results of obstructive sleep apnea (OSA) syndrome subjects. Study Design and Method: Thirty-five subjects who were diagnosed with OSA and underwent sleep surgeries such as septoplasty, tonsillectomy, or uvuloplasty to correct their airway collapse, participated in this study; the watch-PAT-derived respiratory disturbance index (RDI), apnea and hypopnea index (AHI), lowest oxygen saturation, and valid sleep time were measured after the sleep surgery. Results: The present study showed that RDI (32.8 ± 10.7 vs 14.8 ± 7.5), AHI (30.3 ± 8.6 vs 13.4 ± 8.2 events/h), lowest oxygen saturation (78.2% ± 8.4% vs 90.5% ± 7.1%), and valid sleep time (329.1 ± 47.2 min and a postoperative value of 389.1 ± 50.1 min) recovered to within a normal range after surgery in 28 subjects. In addition, good agreement was found between watch-PAT-derived factors and visual analogue scales for changes in subjective symptoms, such as snoring, apnea, and daytime somnolence. Seven of the 35 subjects showed no improvement for their subjective symptoms and complained of snoring and apnea after surgery. We found that the RDI and AHI of those 7 subjects were not reduced, and the changes between pre- and postoperative values which were measured with watch-PAT were minimal. Their postoperative lowest oxygen saturation and valid sleep time were not elevated per the watch-PAT. The results support a strong correlation between the findings from watch-PAT and improved symptoms after surgical correction of an airway collapse. Conclusions: Our study provides evidence that the factors measured by the watch-PAT might be reliable indicators of symptomatic changes in OSA subjects after sleep surgery and also shows that the watch-PAT is a highly sensitive portable device for estimating treatment results in OSA. Citation: Park CY

  10. PAT: a protein analysis toolkit for integrated biocomputing on the web

    PubMed Central

    Gracy, Jérôme; Chiche, Laurent

    2005-01-01

    PAT, for Protein Analysis Toolkit, is an integrated biocomputing server. The main goal of its design was to facilitate the combination of different processing tools for complex protein analyses and to simplify the automation of repetitive tasks. The PAT server provides a standardized web interface to a wide range of protein analysis tools. It is designed as a streamlined analysis environment that implements many features which strongly simplify studies dealing with protein sequences and structures and improve productivity. PAT is able to read and write data in many bioinformatics formats and to create any desired pipeline by seamlessly sending the output of a tool to the input of another tool. PAT can retrieve protein entries from identifier-based queries by using pre-computed database indexes. Users can easily formulate complex queries combining different analysis tools with few mouse clicks, or via a dedicated macro language, and a web session manager provides direct access to any temporary file generated during the user session. PAT is freely accessible on the Internet at . PMID:15980554

  11. The Protein Acetyltransferase PatZ from Escherichia coli Is Regulated by Autoacetylation-induced Oligomerization*

    PubMed Central

    de Diego Puente, Teresa; Gallego-Jara, Julia; Castaño-Cerezo, Sara; Bernal Sánchez, Vicente; Fernández Espín, Vanesa; García de la Torre, José; Manjón Rubio, Arturo; Cánovas Díaz, Manuel

    2015-01-01

    Lysine acetylation is an important post-translational modification in the metabolic regulation of both prokaryotes and eukaryotes. In Escherichia coli, PatZ (formerly YfiQ) is the only known acetyltransferase protein and is responsible for acetyl-CoA synthetase acetylation. In this study, we demonstrated PatZ-positive cooperativity in response to acetyl-CoA and the regulation of acetyl-CoA synthetase activity by the acetylation level. Furthermore, functional analysis of an E809A mutant showed that the conserved glutamate residue is not relevant for the PatZ catalytic mechanism. Biophysical studies demonstrated that PatZ is a stable tetramer in solution and is transformed to its octameric form by autoacetylation. Moreover, this modification is reversed by the sirtuin CobB. Finally, an in silico PatZ tetramerization model based on hydrophobic and electrostatic interactions is proposed and validated by three-dimensional hydrodynamic analysis. These data reveal, for the first time, the structural regulation of an acetyltransferase by autoacetylation in a prokaryotic organism. PMID:26251518

  12. The Protein Acetyltransferase PatZ from Escherichia coli Is Regulated by Autoacetylation-induced Oligomerization.

    PubMed

    de Diego Puente, Teresa; Gallego-Jara, Julia; Castaño-Cerezo, Sara; Bernal Sánchez, Vicente; Fernández Espín, Vanesa; García de la Torre, José; Manjón Rubio, Arturo; Cánovas Díaz, Manuel

    2015-09-18

    Lysine acetylation is an important post-translational modification in the metabolic regulation of both prokaryotes and eukaryotes. In Escherichia coli, PatZ (formerly YfiQ) is the only known acetyltransferase protein and is responsible for acetyl-CoA synthetase acetylation. In this study, we demonstrated PatZ-positive cooperativity in response to acetyl-CoA and the regulation of acetyl-CoA synthetase activity by the acetylation level. Furthermore, functional analysis of an E809A mutant showed that the conserved glutamate residue is not relevant for the PatZ catalytic mechanism. Biophysical studies demonstrated that PatZ is a stable tetramer in solution and is transformed to its octameric form by autoacetylation. Moreover, this modification is reversed by the sirtuin CobB. Finally, an in silico PatZ tetramerization model based on hydrophobic and electrostatic interactions is proposed and validated by three-dimensional hydrodynamic analysis. These data reveal, for the first time, the structural regulation of an acetyltransferase by autoacetylation in a prokaryotic organism.

  13. A game theoretic model of kleptoparasitism with strategic arrivals and departures of beetles at dung pats.

    PubMed

    Barker, Heather A; Broom, Mark; Rychtář, Jan

    2012-05-07

    Dung beetles Onthophagus taurus lay their eggs in brood balls within dung pats. The dung that is used must be sufficiently fresh, and so beetles must keep moving from pat to pat to find fresh dung. If another beetle finds a brood ball it will usually eat the egg inside and lay its own egg in the brood ball instead of constructing its own ball. Thus, beetles will often stay near their eggs to guard them. We model a population of beetles where the times of arrival and departure from pats are strategic choices, and investigate optimal strategies depending upon environmental conditions, which can be reduced to two key parameters, the cost of brood ball construction and the ease of finding balls to parasitise. We predict that beetles should follow one of three distinct behaviors; stay in patches for only short periods, arrive late and be purely parasitic, remain in pats for longer periods in order to guard their brood balls. Under different conditions populations can consist of the first of these types only, a combination of the first and second types, or a combination of all three types.

  14. A novel gene amplification causes upregulation of the PatAB ABC transporter and fluoroquinolone resistance in Streptococcus pneumoniae.

    PubMed

    Baylay, Alison J; Ivens, Alasdair; Piddock, Laura J V

    2015-01-01

    Overexpression of the ABC transporter genes patA and patB confers efflux-mediated fluoroquinolone resistance in Streptococcus pneumoniae and is also linked to pneumococcal stress responses. Although upregulation of patAB has been observed in many laboratory mutants and clinical isolates, the regulatory mechanisms controlling expression of these genes are unknown. In this study, we aimed to identify the cause of high-level constitutive overexpression of patAB in M184, a multidrug-resistant mutant of S. pneumoniae R6. Using a whole-genome transformation and sequencing approach, we identified a novel duplication of a 9.2-kb region of the M184 genome which included the patAB genes. This duplication did not affect growth and was semistable with a low segregation rate. The expression levels of patAB in M184 were much higher than those that could be fully explained by doubling of the gene dosage alone, and inactivation of the first copy of patA had no effect on multidrug resistance. Using a green fluorescent protein reporter system, increased patAB expression was ascribed to transcriptional read-through from a tRNA gene upstream of the second copy of patAB. This is the first report of a large genomic duplication causing antibiotic resistance in S. pneumoniae and also of a genomic duplication causing antibiotic resistance by a promoter switching mechanism.

  15. Communicating with New and Existing Markets. A Task Force of Howard Community College's 1998-1999 Commission on the Future.

    ERIC Educational Resources Information Center

    Howard Community Coll., Columbia, MD.

    This is a report from a task force formed by Howard Community College (Maryland) to examine existing and future markets. The task force also explored ways to use marketing strategies to attract potential customers to the college. The task force recommends that the college use its strengths to attract customers, such as its commitment to open…

  16. The Power-Load-Margin Formula of Howard Y. McClusky as the Basis for a Model of Teaching.

    ERIC Educational Resources Information Center

    Main, Keith

    1979-01-01

    Examines the writings of Howard McClusky and his power-load-margin (PLM) formula as the nucleus for a teaching-learning model that fosters mutual respect, shared responsibility, and a spirit of mutual inquiry in small groups or individualized learning experiences. Four phases which form the syntax of the PLM model are presented. (LRA)

  17. Capacity-Building for African American Mental Health Training and Research: Lessons from the Howard-Dartmouth Collaborative Summer School

    ERIC Educational Resources Information Center

    Hipolito, Maria M. S.; Malik, Mansoor; Carpenter-Song, Elizabeth; Whitley, Rob

    2012-01-01

    Background: Many psychiatric residents have traditionally received little-or-no training in cross cultural approaches to psychiatric training and research. Method: The Dartmouth-Howard Collaboration summer school training program had a 5-year grant to explore approaches to enhancing understanding of cultural factors in mental health treatment and…

  18. Howard Joseph McKee, Jr., Winnebago Traditionalist. With Teacher's Guide. Native Americans of the Twentieth Century.

    ERIC Educational Resources Information Center

    Minneapolis Public Schools, MN.

    A biography for elementary school students describes Howard Joseph McKee, Jr, an American Indian elder who teaches the Winnebago language and is developing a system for translating it into English. Mr. McKee's photograph and a map of Nebraska reservations are included. A teaching guide following the bibliography contains learning objectives and…

  19. The Academic Tree of Howard V. Malmstadt: From Early Scientific Exploration to Modern Analytical Chemistry.

    PubMed

    Storey, Andrew P; Hieftje, Gary M

    2016-12-01

    Over the last several decades, science has benefited tremendously by the implementation of digital electronic components for analytical instrumentation. A pioneer in this area of scientific inquiry was Howard Malmstadt. Frequently, such revolutions in scientific history can be viewed as a series of discoveries without a great deal of attention as to how mentorship shapes the careers and methodologies of those who made great strides forward for science. This paper focuses on the verifiable relationships of those who are connected through the academic tree of Malmstadt and how their experiences and the context of world events influenced their scientific pursuits. Particular attention is dedicated to the development of American chemistry departments and the critical role played by many of the individuals in the tree in this process.

  20. Report of the Howard Hughes Medical Institute's workshop on the performance of laboratory chemical hoods.

    PubMed

    DiBerardinis, Louis J; First, Melvin W; Party, Esmeralda; Smith, Thomas C; Warfield, Cheryl A; Carpenter, J Patrick; Cook, J Lindsay; Walters, Douglas B; Flynn, Michael R; Galson, Edgar L; Greenley, Pamela L; Hitchings, Dale T; Knutson, Gerhard W; Price, John M; Baum, Janet S; Burton, Jeff D; Finucane, Matthew D; Ghidoni, Daniel A; Koenigsberg, Jerry; Lyons, Mark; Memarzadeh, Farhad; Norton, David C; Schuyler, Glen; Zboralski, Jon; Barkley, W Emmett

    2003-01-01

    The Howard Hughes Medical Institute sponsored a workshop on laboratory chemical hoods on June 8, 9, and 10, 1998, that brought together 24 experts in the field of laboratory chemical hoods to critically assess the information known about hood performance. Workshop participants developed 31 consensus statements that reflect their collective views on the body of knowledge or lack thereof, for laboratory chemical hoods. The consensus statements fall into four broad categories: (1) hood selection, use, and operation; (2) hood and laboratory design issues; (3) ventilation system design issues; and (4) hood performance testing. The consensus statements include 26 statements on what is known and unknown about the performance of laboratory chemical hoods, 2 statements of definition, and 3 statements that reflect the participants' agreement not to agree. The brief commentary that follows each consensus statement provides guidance and recommendations.

  1. Mosquitoes, flies and dental cavities: Dr. Howard Riley Raper's public campaign to prevent toothache.

    PubMed

    Christen, Arden G; Christen, Joan A

    2010-01-01

    Dr. Howard Riley Raper (1886-1978) was an early oral health pioneer and dental roentgenology faculty member of the Indiana Dental College (IDC) who single-handedly introduced key concepts in radiology to dentistry. Due to his efforts, IDC became in 1910-11 the first dental school to have a regular course in dental radiology. Virtually all American dental schools soon added this subject to their regular curriculum. Raper's text, Elementary and Dental Radiography (1913) became the first comprehensive student textbook of dental X-ray diagnosis. In his 1933 Blue Book entitled, The New Aim in the Care of the Teeth, Raper elaborated upon his mission to prevent caries, by comparing the insidious damages of tooth decay with the threat of insect-borne disease.

  2. Water Vapor Measurements by Howard University Raman Lidar during the WAVES 2006 Campaign

    NASA Technical Reports Server (NTRS)

    Adam, M.; Demoz, B. B.; Whiteman, D. N.; Venable, D. D.; Joseph E.; Gambacorta, A.; Wei, J.; Shephard, M. W.; Miloshevich, L. M.; Barnet, C. D.; Herman, R. L.; Fitzgibbon, J.; Connell, R.

    2009-01-01

    Retrieval of water vapor mixing ratio using the Howard University Raman Lidar is presented with emphasis on three aspects: i) performance of the lidar against collocated radiosondes and Raman lidar, ii) investigation of the atmospheric state variables when poor agreement between lidar and radiosondes values occurred and iii) a comparison with satellite-based measurements. The measurements were acquired during the Water Vapor Validation Experiment Sondes/Satellites 2006 field campaign. Ensemble averaging of water vapor mixing ratio data from ten night-time comparisons with Vaisala RS92 radiosondes shows on average an agreement within 10 % up to approx. 8 km. A similar analysis of lidar-to-lidar data of over 700 profiles revealed an agreement to within 20 % over the first 7 km (10 % below 4 km). A grid analysis, defined in the temperature - relative humidity space, was developed to characterize the lidar - radiosonde agreement and quantitatively localizes regions of strong and weak correlations as a function of altitude, temperature or relative humidity. Three main regions of weak correlation emerge: i) regions of low relative humidity and low temperature, ii) moderate relative humidity at low temperatures and iii) low relative humidity at moderate temperatures. Comparison of Atmospheric InfraRed Sounder and Tropospheric Emission Sounder satellites retrievals of moisture with that of Howard University Raman Lidar showed a general agreement in the trend but the formers miss a lot of the details in atmospheric structure due to their low resolution. A relative difference of about 20 % is usually found between lidar and satellites measurements.

  3. Overview of the High Performance Antiproton Trap (HiPAT) Experiment

    NASA Technical Reports Server (NTRS)

    Martin, James; Chakrabarti, Suman; Pearson, Boise; Sims, W. Herbert; Lewis, Raymond; Fant, Wallace; Rodgers, Stephen (Technical Monitor)

    2002-01-01

    A general overview of the High Performance Antiproton Trap (HiPAT) Experiment is presented. The topics include: 1) Why Antimatter? 2) HiPAT Applicability; 3) Approach-Goals; 4) HiPAT General Layout; 5) Sizing For Containment; 6) Laboratory Operations; 7) Vacuum System Cleaning; 8) Ion Production Via Electron Gun; 9) Particle Capture Via Ion Sources; 10) Ion Beam Steering/Focusing; 11) Ideal Ion Stacking Sequence; 12) Setup For Dynamic Capture; 13) Dynamic Capture of H(+) Ions; 14) Dynamic Capture; 15) Radio Frequency Particle Detection; 16) Radio Frequency Antenna Modeling; and 17) R.F. Stabilization-Low Frequencies. A short presentation of propulsion applications of Antimatter is also given. This paper is in viewgraph form.

  4. RF Manipulation and Detection of Protons in the High Performance Antiproton Trap (HiPAT) Experiment

    NASA Technical Reports Server (NTRS)

    Martin, James; Lewis, Raymond A.; Pearson, J. Boise; Sims, W. Herb; Chakrabarti, Suman; Fant, Wallace E.; Stan McDonald

    2003-01-01

    HiPAT is a Penning-Malmberg trap designed to contain 10(exp 12) particles diagnostics of the plasma. Destructive particle detection is performed by extracting trapped ions from HiPAT and colliding them with a microchannel plate detector (providing number and energy information). The improved RF system has been used to detect various plasma modes for both electron and ion plasmas in the two traps at MSFC, including axd, cyclotron, and diocotron modes. New diagnostics are also being added to HiPAT to measure the axial density distribution of the trapped cloud to match measured RF plasma modes to plasma conditions. In addition, plasma modeling efforts have been started using the XOOPIC code.

  5. The effects of PAT on the Savannah River ecosystem, particularly fisheries

    SciTech Connect

    Patrick, R.

    1994-03-01

    The main purpose of this study was to determine whether or not the pre-startup activities at K-Reactor, i.e., Power Ascension Testing (PAT), have caused damage because of temperature rises in the Savannah River. Therefore, the biological studies were mainly aimed at providing information as to changes that might cause the damage of the fish population, and to other important organisms in the ecosystem. To determine if deleterious effects had occurred, one had to review the past studies to determine the condition and diversity of aquatic life before these PAT studies started. Therefore old reports were reviewed and a current study made in 1992.

  6. Reconstruction of undersampled radial PatLoc imaging using Total Generalized Variation

    PubMed Central

    Knoll, Florian; Schultz, Gerrit; Bredies, Kristian; Gallichan, Daniel; Zaitsev, Maxim; Hennig, Jürgen; Stollberger, Rudolf

    2016-01-01

    In the case of radial imaging with nonlinear spatial encoding fields, a prominent star-shaped artifact has been observed if a spin distribution is encoded with an undersampled trajectory. This work presents a new iterative reconstruction method based on the total generalized variation (TGV), which reduces this artifact. For this approach, a sampling operator (as well as its adjoint) is needed that maps data from PatLoc k-space to the final image space. It is shown that this can be realized as a Type-3 non-uniform FFT, which is implemented by a combination of a Type-1 and Type-2 non-uniform FFT. Using this operator, it is also possible to implement an iterative conjugate gradient (CG) SENSE based method for PatLoc reconstruction, which leads to a significant reduction of computation time in comparison to conventional PatLoc image reconstruction methods. Results from numerical simulations and in-vivo PatLoc measurements with as few as 16 radial projections are presented, which demonstrate significant improvements in image quality with the TGV based approach. PMID:22847824

  7. Design and Fabrication of Cryostat Interface and Electronics for High Performance Antimatter Trap (HI-PAT)

    NASA Technical Reports Server (NTRS)

    Smith, Gerald A.

    1999-01-01

    Included in Appendix I to this report is a complete set of design and assembly schematics for the high vacuum inner trap assembly, cryostat interfaces and electronic components for the MSFC HI-PAT. Also included in the final report are summaries of vacuum tests, and electronic tests performed upon completion of the assembly.

  8. Draft Genome Sequence of the Beer Spoilage Bacterium Megasphaera cerevisiae Strain PAT 1T

    PubMed Central

    Kutumbaka, Kirthi K.; Pasmowitz, Joshua; Mategko, James; Reyes, Dindo; Friedrich, Alex; Han, Sukkyun; Martens-Habbena, Willm; Neal-McKinney, Jason; Janagama, Harish K.; Nadala, Cesar

    2015-01-01

    The genus Megasphaera harbors important spoilage organisms that cause beer spoilage by producing off flavors, undesirable aroma, and turbidity. Megasphaera cerevisiae is mainly found in nonpasteurized low-alcohol beer. In this study, we report the draft genome of the type strain of the genus, M. cerevisiae strain PAT 1T. PMID:26358606

  9. Investigating Trauma in Narrating World War I: A Psychoanalytical Reading of Pat Barker's "Regeneration"

    ERIC Educational Resources Information Center

    Sadjadi, Bakhtiar; Esmkhani, Farnaz

    2016-01-01

    The present paper seeks to critically read Pat Barker's "Regeneration" in terms of Cathy Caruth's psychoanalytic study of trauma. This analysis attempts to trace the concepts of latency, post-traumatic stress disorders, traumatic memory, and trauma in Barker's novel in order to explore how trauma and history are interrelated in the…

  10. Synchronized fission yeast meiosis using an ATP analog-sensitive Pat1 protein kinase.

    PubMed

    Cipak, Lubos; Polakova, Silvia; Hyppa, Randy W; Smith, Gerald R; Gregan, Juraj

    2014-01-01

    Synchronous cultures are often indispensable for studying meiosis. Here we present an optimized protocol for induction of synchronous meiosis in the fission yeast Schizosaccharomyces pombe. Chemical inactivation of an ATP analog-sensitive form of the Pat1 kinase (pat1-as2) by adding the ATP analog 1-NM-PP1 in G1-arrested cells allows the induction of synchronous meiosis at optimal temperature (25°C). Importantly, this protocol eliminates detrimental effects of elevated temperature (34°C), which is required to inactivate the commonly used temperature-sensitive Pat1 kinase mutant (pat1-114). The addition of the mat-Pc gene to a mat1-M strain further improves chromosome segregation and spore viability. Thus, our protocol offers highly synchronous meiosis at optimal temperature, with most characteristics similar to those of wild-type meiosis. The synchronization protocol can be completed in 5 d (not including strain production, which may take as long as 2 or 3 months).

  11. Teacher's PAT? Multiple-Role Principal-Agent Theory, Education Politics, and Bureaucrat Power

    ERIC Educational Resources Information Center

    Vanhuysse, Pieter; Sulitzeanu-Kenan, Raanan

    2009-01-01

    This article aims to contribute to current debates about political power and agency relationships in education and other public sectors. In a recent clarion call for a major redirection of political principal-agent theories (PAT), Terry Moe has argued that standard information asymmetries ought no longer to be regarded as the sole foundation of…

  12. Quantifying beetle-mediated effects on gas fluxes from dung pats.

    PubMed

    Penttilä, Atte; Slade, Eleanor M; Simojoki, Asko; Riutta, Terhi; Minkkinen, Kari; Roslin, Tomas

    2013-01-01

    Agriculture is one of the largest contributors of the anthropogenic greenhouse gases (GHGs) responsible for global warming. Measurements of gas fluxes from dung pats suggest that dung is a source of GHGs, but whether these emissions are modified by arthropods has not been studied. A closed chamber system was used to measure the fluxes of carbon dioxide (CO2), methane (CH4) and nitrous oxide (N2O) from dung pats with and without dung beetles on a grass sward. The presence of dung beetles significantly affected the fluxes of GHGs from dung pats. Most importantly, fresh dung pats emitted higher amounts of CO2 and lower amounts of CH4 per day in the presence than absence of beetles. Emissions of N2O showed a distinct peak three weeks after the start of the experiment--a pattern detected only in the presence of beetles. When summed over the main grazing season (June-July), total emissions of CH4 proved significantly lower, and total emissions of N2O significantly higher in the presence than absence of beetles. While clearly conditional on the experimental conditions, the patterns observed here reveal a potential impact of dung beetles on gas fluxes realized at a small spatial scale, and thereby suggest that arthropods may have an overall effect on gas fluxes from agriculture. Dissecting the exact mechanisms behind these effects, mapping out the range of conditions under which they occur, and quantifying effect sizes under variable environmental conditions emerge as key priorities for further research.

  13. Project-based Modules from two STEM Learning Teams in Howard County, Maryland

    NASA Astrophysics Data System (ADS)

    Griffiths, L. N.; Bradley, L. A.

    2011-12-01

    In 2009, two Maryland school districts-Howard County Public School System and Prince George's County Public Schools-and the Goddard Space Flight Center of the National Aeronautics and Space Administration (NASA) partnered with the National Commission on Teaching and America's Future (NCTAF) to develop NASA 21st Century Learning Studios. In 2010, NCTAF expanded the program to include Learning Studios at two additional Maryland school districts (Anne Arundel County Public Schools and Baltimore County Public Schools), partnering with the United States Naval Academy and the University of Maryland. Overall, the focus of these Learning Studios is to combine the expertise of scientists with that of educators through Learning Teams to improve teaching and learning in science, technology, engineering and mathematics (STEM) fields, while delivering project-based modules to be implemented in other school districts. The focus of this paper is to summarize the experience and outcomes from two Learning Teams from the Howard County Public School System. STEM Learning Teams were established at Centennial High School and Hammond High School in Maryland. Each Team worked together for two years to create interdisciplinary units of study for their students with a focus on Earth Science. To maximize student interest, teachers worked with NASA scientists five times a year to develop four learning modules using practical examples and incorporating real scientific observations. A weathering and erosion module challenges students to collect appropriate field observations and determine erosion and deposition rates in a nearby lake. A plate tectonics module requires students to use measures of plate motion from the National Oceanic and Atmospheric Administration to estimate rates of convergence in southern Asia. A third module for lessons in climate change requires students to find open source climate data, determine changes in the atmosphere and estimate anthropogenic impacts. A follow

  14. Healing mysteries: An interview with Howard Hall, PhD, PsyD. Interview by Sheldon Lewis.

    PubMed

    Hall, Howard

    2007-01-01

    Dr Howard Hall is an associate professor in the department of pediatrics at Case Western Reserve University (CWRU) and an attending doctor in the division of behavioral pediatrics at Rainbow Babies and Children's Hospital in Cleveland, Ohio. Dr Hall holds a PhD in Experimental Psychology from Princeton University and a PsyD in Clinical Psychology from Rutgers University. He has conducted research and taught courses in clinical and multicultural psychology and maintains a clinical practice using hypnosis and other mind-body approaches to healing at CWRU. Howard Hall has been recognized as a leader in the field of clinical psychoneuroimmunology and conducted pioneering research on the effects of hypnosis on immune responses. In recent years, he has studied energy-based rapid wound healing as demonstrated by Sufi practitioners. Recently, Sheldon Lewis, Editor-in-Chief of Advances in Mind-Body Medicine, spoke with Dr Hall about his work.

  15. 2002 Report to Congress: Evaluating the Consensus Best Practices Developed through the Howard Hughes Medical Institute’s Collaborative Hazardous Waste Management Demonstration Project

    EPA Pesticide Factsheets

    This report discusses a collaborative project initiated by the Howard Hughes Medical Institute (HHMI) to establish and evaluate a performance-based approach to management of hazardous wastes in the laboratories of academic research institutions.

  16. Comparative sound velocity measurements between porous rock and fully-dense material under crustal condition: The cases of Darley Dale sandstone and copper block

    NASA Astrophysics Data System (ADS)

    Kung, J.; Chien, Y. V.; Wu, W.; Dong, J.; Chang, Y.; Tsai, C.; Yang, M.; Wang, K.

    2012-12-01

    Previous studies showed that the voids and their geometry in the sedimentary rocks have great influence on the compressibility of rock, which reflects on its elastic velocities. Some models were developed to discuss the relations among velocity, porosity and void geometry. Therefore, the information of porosity, and void geometry and its distribution in rock is essential for understanding how the elastic properties of porous rocks affected by their poregeometry. In this study, we revisited a well-studied porous rock, Darley Dale sandstone, which has been studied by different groups with different purposes. Most of them are the deformation experiments. Different from previous studies, we measured the sound velocity of Darley dale sandstone under hydrostatic conditions. Also, we employed different techniques to investigate the pore geometry and porosity of Darley Dale sandstone to gain the insight of velocity changing behavior under the crustal conditions. Here, we measured a fully-dense copper block for a comparison. We performed X-ray CT scanning (XCT) to image the pore space of sandstone to construct the 3-D image of pore geometry, distribution and the pore size. The CT image data are allowed us to estimate the porosity of sandstone, too. One the other hand, the porosity of sample was measured using imbibitions method at ambient conditions and helium porosimeter at high pressure (up to 150 MPa). A set of specimens were cored from Darley Dale sandstone block. P and S wave velocities of specimens were measured at ambient conditions. We also performed high pressure velocity measurements on a selected rock specimen and a copper block up to 150 MPa under dry condition. Porosity of a set of rock specimens measured by imbibitions method was spanned from 6% to 15%, largely distributed within a range of 8%-11%. Compared the porosity obtained from three different techniques, imbibitions method, helium porosimeter and XCT, values from those measurements are in good agreement

  17. Cannabis careers revisited: applying Howard S. Becker's theory to present-day cannabis use.

    PubMed

    Järvinen, Margaretha; Ravn, Signe

    2014-01-01

    A considerable part of today's sociological research on recreational drug use is (explicitly or implicitly) inspired by Howard Becker's classical model of deviant careers. The aim of the present paper is to directly apply Becker's theory to empirical data on present-day cannabis use and to suggest a revision of the theory. As part of this, we propose a stretch of the sociological approach represented by Becker and followers in order to include, not only recreational drug use, but also use for which young people have sought treatment. The paper is based on 30 qualitative interviews with young people in treatment for cannabis problems in Copenhagen, Denmark. We suggest a revision of Becker's career model in relation to four aspects: initiation of cannabis use, differentiation between socially integrated and individualised, disintegrated use, social control from non-users, and the users' moral stance on cannabis. A central point of the paper is that social interaction may both motivate cannabis use, as Becker proposed, and serve as a protective factor against extensive, problematic use.

  18. Evaluation of waterflood operations at Iatan East Howard Field, Mitchell County, Texas

    SciTech Connect

    Smith, D.P.; Mitchell, S.M.

    1988-02-01

    Mobil Exploration and Producing US Inc. completed a reservoir description study on Iatan East Howard field in Mitchell County, Texas, in 1984. The application of study results has improved subsequent development drilling and waterflood operations. Lease production has doubled within two years with the drilling of 40 producers and 13 injection wells. The field produces from thin (2 to 40 ft) porosity stringers in fractured Permian age San Angelo and Clearfork dolomites at 2300 to 3200 ft in depth. Reservoirs are areally discontinuous due to original depositional controls on porosity develoment and distribution. Pay zone reservoir parameters such as porosity and permeability vary from 4 to 17% and 0.1 to 120 md, respectively. The presence of a fracture orientation at N60/degree/E to N85/degree/E has caused producing wells to experience early breakthrough of injection water when in line with injectors and the fracture direction. Waterflows have also occurred in drilling wells when similarly aligned. Recognition of this fracture overprint has dictated the use of a staggered line drive injection pattern parallel to the fracture trend to improve sweep efficiency.

  19. Evaluation of waterflood operations at Iatan East Howard field, Mitchell County, Texas

    SciTech Connect

    Smith, D.P.; Mitchell, S.M.

    1988-01-01

    Mobil Exploration and Producing U.S. Inc. completed a reservoir description study on Iatan East Howard field in Mitchell County, Texas, in 1984. The application of study results has improved subsequent development drilling and waterflood operations. Lease production has doubled within two years with the drilling of 40 producers and 13 injection wells. The field produces from thin (2 to 40 ft) porosity stringers in fractured Permian age San Angelo and Clearfork dolomites at 2,300 to 3,200 ft in depth. Reservoirs are really discontinuous due to original depositional controls on porosity development and distribution. Pay zone reservoir parameters such as porosity and permeability vary from 4 to 17% and 0.1 to 120 md, respectively. The presence of a fracture orientation at N60/sup 0/E to N85/sup 0/E has caused producing wells to experience early breakthrough of injection water when in line with injectors and the fracture direction. Waterflows have also occurred in drilling wells when similarly aligned. Recognition of this fracture overprint has dictated the use of a staggered line drive injection pattern parallel to the fracture trend to improve sweep efficiency. Reservoir matrix can be swept more uniformly as rows of injectors pressurize those aligned fractures and move oil perpendicular to the fracture trend and toward the rows of producers.

  20. B. C. Canyon field, Howard County, Texas: An ancient analogy to modern tropical tower karst terrains

    SciTech Connect

    Mozynski, D.C.; Reid, A.M. )

    1992-04-01

    Late in the early deposition of sediments in Canyon field, a series of glacio-eustatically controlled sea level lowstands resulted in a carbonate buildup seaward of the Horseshoe Atoll in Howard County, Texas. The resulting satellite reef tract consists of fringing boundstone; high-energy shelf grainstones; lower energy shelf packstones and wackestones; and thin, highstand, black shales and mudstones. The original extent and thickness of deposits were extensively modified during karstification coincident with successive sea level lowstands. The resulting paleotopographic landforms appear to be similar to tower karst features of Puerto Rico. During the beginning of each sea level highstand, the paleoterrain was modified by erosion. The basinal foreshelf conglomerates resulting from initial highstand erosion contain dipping strata that commonly can be detected by the dipmeter tool. The mechanism for the formation of these strata may be depositional or the result of diagenetic alteration of the rock fabric in the burial environment. Using dipmeter data, an uneconomic producer has been offset by one of the better producing wells in the field.

  1. Application of a novel phosphinothricin N-acetyltransferase (RePAT) gene in developing glufosinate-resistant rice

    PubMed Central

    Cui, Ying; Liu, Ziduo; Li, Yue; Zhou, Fei; Chen, Hao; Lin, Yongjun

    2016-01-01

    Currently, only few glufosinate-resistant genes are available for commercial application. Thus, developing novel glufosinate-resistant genes with commercial feasibility is extremely important and urgent for agricultural production. In this study, we transferred a newly isolated RePAT gene into a japonica rice variety Zhonghua11, resulting in a large number of independent T0 transgenic plants, most of which grew normally under high-concentration glufosinate treatment. Four transgenic plants with one intact RePAT expression cassette integrated into the intergenic region were selected. Agronomic performances of their T2 progenies were investigated, and the results suggested that the expression of RePAT had no adverse effect on the agronomic performance. Definite glufosinate resistance of the selected transgenic plants was further confirmed to be related to the expression of RePAT by assay on the medium and qRT-PCR. The inheritance and expression of RePAT in two transgenic plants were confirmed to be stable. Finally, the two-year field assay of glufosinate resistance suggested that the agronomic performance of the transgenic plant (PAT11) was not affected by high dosage of glufosinate (5000 g/ha). Collectively, our study proves the high resistance of a novel gene RePAT to glufosinate and provides a glufosiante-resistant rice variety with agricultural application potential. PMID:26879398

  2. An Evaluation of the LOS Alamos Precision Automated Turning System (pats) as a Production Tool for Atlas Liners

    NASA Astrophysics Data System (ADS)

    Anderson, W.; Day, R.; Hatch, D.; Gore, R.; Machen, D.; Bartos, J.; Salazar, M.; Hannah, P.

    2004-11-01

    The PATS is proposed as a possible means for efficient production of precision liners for Atlas [1]. The Los Alamos National Laboratory's High Energy Density Physics (HEDP) program supported an evaluation of this prospect over the last two years. The machine operations have been carefully mapped out, the status of the controllers and on-machine gauging evaluated, and dynamic error addressed. The PATS has been used to generate diamond turned liners with dimensions similar to those proposed for Atlas. In the mean time, Atlas liner criteria have become even more stringent. This paper will evaluate the known status of the PATS in view of current liner designs for Atlas.

  3. PAT tools for the control of co-extrusion implants manufacturing process.

    PubMed

    Krier, Fabrice; Mantanus, Jérôme; Sacré, Pierre-Yves; Chavez, Pierre-François; Thiry, Justine; Pestieau, Aude; Rozet, Eric; Ziemons, Eric; Hubert, Philippe; Evrard, Brigitte

    2013-12-15

    Hot melt extrusion is a novel pharmaceutical manufacturing process technique. In this study, we identified four Critical Quality Attributes (CQAs) of the implant manufacturing process by hot melt extrusion: the implant diameter, the quantity of the Active Pharmaceutical Ingredient (API), the homogeneity distribution of API and the thickness of the membrane. We controlled the implant diameter and the quantity of API in-line with a laser measurement, NIR and Raman spectroscopy, respectively. These two different spectroscopic techniques provided comparable results. In fact, the RMSEC and RMSECV were very close in each PAT technique but NIR spectroscopy was easier to use and less sensitive to external changes. For the control of the homogeneity of API distribution and the thickness of the membrane, we used successfully Raman spectroscopy imaging. These PAT tools help reducing analysis time.

  4. Review of the High Performance Antiproton Trap (HiPAT) Experiment

    NASA Technical Reports Server (NTRS)

    Martin, James J.; Lewis, Raymond A.; Pearson, J. Boise; Sims, W. Herb; Chakrabarti, Suman; Fant, Wallace E.; McDonald, Stan

    2003-01-01

    Many space propulsion concepts exist that use matter-antimatter reactions. Current antiproton production rates are enough to conduct proof-of-principle evaluation of these concepts. One enabling technology for such experiments is portable storage of low energy antiprotons, to transport antiprotons to experimental facilities. To address this need, HiPAT is being developed, with a design goal of containing 10(exp 12) particles for up to 18 days. HiPAT is a Penning-Malmberg trap with a 4 Tesla superconductor, 20kV electrodes, radio frequency (RF) network, and 10(exp -13) Torr vacuum. 'Normal' matter is being used to evaluate the system. An electron beam ionizes background gas in situ, and particle beams are captured dynamically. The experiment examines ion storage lifetimes, RF plasma diagnostics, charge exchange with background gases, and dynamic ion beam capture.

  5. PAT-2 (Plutonium Air-Transportable Model 2) safety analysis report

    SciTech Connect

    Andersen, J.A.; Davis, E.J.; Duffey, T.A.; Dupree, S.A.; George, O.L. Jr.; Ortiz, Z.

    1981-07-01

    The PAT-2 package is designed for the safe transport of plutonium and/or uranium in small quantities. The PAT-2 package is resistant to severe accidents, including that of a high-speed jet aircraft crash, and is designed to withstand such environments as extreme impact, crushing, puncturing and slashing loads, severe hydrocarbon-fueled fires, and deep underwater immersion, with no escape of contents. The package meets the requirements of 10 CFR 71 for Fissile Class I packages with a cargo of 15 grams of Pu-239, or other isotopic forms described herein, not to exceed 2 watts of thermal activity. This SAR presents design and oprational information including evaluations and analyses, test results, operating procedures, maintenance, and quality assurance information.

  6. PAT-1 safety analysis report addendum author responses to request for additional information.

    SciTech Connect

    Weiner, Ruth F.; Schmale, David T.; Kalan, Robert J.; Akin, Lili A.; Miller, David Russell; Knorovsky, Gerald Albert; Yoshimura, Richard Hiroyuki; Lopez, Carlos; Harding, David Cameron; Jones, Perry L.; Morrow, Charles W.

    2010-09-01

    The Plutonium Air Transportable Package, Model PAT-1, is certified under Title 10, Code of Federal Regulations Part 71 by the U.S. Nuclear Regulatory Commission (NRC) per Certificate of Compliance (CoC) USA/0361B(U)F-96 (currently Revision 9). The National Nuclear Security Administration (NNSA) submitted SAND Report SAND2009-5822 to NRC that documented the incorporation of plutonium (Pu) metal as a new payload for the PAT-1 package. NRC responded with a Request for Additional Information (RAI), identifying information needed in connection with its review of the application. The purpose of this SAND report is to provide the authors responses to each RAI. SAND Report SAND2010-6106 containing the proposed changes to the Addendum is provided separately.

  7. Quantifying Beetle-Mediated Effects on Gas Fluxes from Dung Pats

    PubMed Central

    Penttilä, Atte; Slade, Eleanor M.; Simojoki, Asko; Riutta, Terhi; Minkkinen, Kari; Roslin, Tomas

    2013-01-01

    Agriculture is one of the largest contributors of the anthropogenic greenhouse gases (GHGs) responsible for global warming. Measurements of gas fluxes from dung pats suggest that dung is a source of GHGs, but whether these emissions are modified by arthropods has not been studied. A closed chamber system was used to measure the fluxes of carbon dioxide (CO2), methane (CH4) and nitrous oxide (N2O) from dung pats with and without dung beetles on a grass sward. The presence of dung beetles significantly affected the fluxes of GHGs from dung pats. Most importantly, fresh dung pats emitted higher amounts of CO2 and lower amounts of CH4 per day in the presence than absence of beetles. Emissions of N2O showed a distinct peak three weeks after the start of the experiment – a pattern detected only in the presence of beetles. When summed over the main grazing season (June–July), total emissions of CH4 proved significantly lower, and total emissions of N2O significantly higher in the presence than absence of beetles. While clearly conditional on the experimental conditions, the patterns observed here reveal a potential impact of dung beetles on gas fluxes realized at a small spatial scale, and thereby suggest that arthropods may have an overall effect on gas fluxes from agriculture. Dissecting the exact mechanisms behind these effects, mapping out the range of conditions under which they occur, and quantifying effect sizes under variable environmental conditions emerge as key priorities for further research. PMID:23940758

  8. A numerical model characterizing the experimental performance of the Howard University Raman Lidar system

    NASA Astrophysics Data System (ADS)

    Connell, Rasheen M.

    At the Howard University Atmospheric Observatory in Beltsville, MD, a Raman Lidar System was developed to provide both daytime and nighttime measurements of water vapor, aerosols, and cirrus clouds with 60 s temporal and 7.5 m spatial resolution in the lower and upper troposphere. This system analyzes signals at three wavelengths associated with Rayleigh/Mie scattering for aerosols and cirrus clouds at 354.7 nm, Raman scattering for nitrogen at 386.7 nm, and water vapor at 407.5 nm. The transmitter is a triple harmonic Nd: YAG solid state laser. The receiver is a 40 cm Cassegrain telescope. The detector system consists of a multi-channel wavelength separator unit and data acquisition system. This thesis develops a numerical model to provide a realistic representation of the system behavior. The variants of the lidar equation in the model use system parameters to solve and determine the return signals for the lidar system. This dissertation describes four case studies being investigated: clear sky, polluted, wet, and cirrus cloud atmospheric conditions. The first simulations are based on a standard atmosphere, which assumes an unpolluted (aerosol-free) dry-air atmosphere. The second and third sets of simulations are based on polluted and cirrus cloud atmospheric conditions, where aerosols and cirrus clouds are added to Case Study I. The last set of simulations is based on a wet atmosphere, where the troposphere is comprised of the same mixture of gases in Case Study II, with the addition of atmospheric water vapor. Lidar signals are simulated over the altitude range covered by our measurements (up to 14 km). Results of our simulations show that the measured and modeled signals agree within 10% over an extended period of time when the system (i.e., such as alignment, filter tuning, etc.) has not changed.

  9. X-PAT: a multiplatform patient referral data management system for small healthcare institution requirements.

    PubMed

    Masseroli, Marco; Marchente, Mario

    2008-07-01

    We present X-PAT, a platform-independent software prototype that is able to manage patient referral multimedia data in an intranet network scenario according to the specific control procedures of a healthcare institution. It is a self-developed storage framework based on a file system, implemented in eXtensible Markup Language (XML) and PHP Hypertext Preprocessor Language, and addressed to the requirements of limited-dimension healthcare entities (small hospitals, private medical centers, outpatient clinics, and laboratories). In X-PAT, healthcare data descriptions, stored in a novel Referral Base Management System (RBMS) according to Health Level 7 Clinical Document Architecture Release 2 (CDA R2) standard, can be easily applied to the specific data and organizational procedures of a particular healthcare working environment thanks also to the use of standard clinical terminology. Managed data, centralized on a server, are structured in the RBMS schema using a flexible patient record and CDA healthcare referral document structures based on XML technology. A novel search engine allows defining and performing queries on stored data, whose rapid execution is ensured by expandable RBMS indexing structures. Healthcare personnel can interface the X-PAT system, according to applied state-of-the-art privacy and security measures, through friendly and intuitive Web pages that facilitate user acceptance.

  10. PatMatch: a program for finding patterns in peptide and nucleotide sequences

    PubMed Central

    Yan, Thomas; Yoo, Danny; Berardini, Tanya Z.; Mueller, Lukas A.; Weems, Dan C.; Weng, Shuai; Cherry, J. Michael; Rhee, Seung Y.

    2005-01-01

    Here, we present PatMatch, an efficient, web-based pattern-matching program that enables searches for short nucleotide or peptide sequences such as cis-elements in nucleotide sequences or small domains and motifs in protein sequences. The program can be used to find matches to a user-specified sequence pattern that can be described using ambiguous sequence codes and a powerful and flexible pattern syntax based on regular expressions. A recent upgrade has improved performance and now supports both mismatches and wildcards in a single pattern. This enhancement has been achieved by replacing the previous searching algorithm, scan_for_matches [D'Souza et al. (1997), Trends in Genetics, 13, 497–498], with nondeterministic-reverse grep (NR-grep), a general pattern matching tool that allows for approximate string matching [Navarro (2001), Software Practice and Experience, 31, 1265–1312]. We have tailored NR-grep to be used for DNA and protein searches with PatMatch. The stand-alone version of the software can be adapted for use with any sequence dataset and is available for download at The Arabidopsis Information Resource (TAIR) at . The PatMatch server is available on the web at for searching Arabidopsis thaliana sequences. PMID:15980466

  11. Ion Dynamic Capture Experiments With The High Performance Antiproton Trap (HiPAT)

    NASA Technical Reports Server (NTRS)

    Martin, James; Lewis, Raymond; Chakrabarti, Suman; Sims, William H.; Pearson, J. Boise; Fant, Wallace E.

    2002-01-01

    To take the first step towards using the energy produced from the matter-antimatter annihilation for propulsion applications, the NASA Marshall Space Flight Center (MSFC) Propulsion Research Center (PRC) has initiated a research activity examining the storage of low energy antiprotons. The High Performance Antiproton Trap (HiPAT) is an electromagnetic system (Penning-Malmberg design) consisting of a 4 Tesla superconductor, a high voltage electrode confinement system, and an ultra high vacuum test section. It has been designed with an ultimate goal of maintaining 10(exp 12) charged particles with a half-life of 18 days. Currently, this system is being evaluated experimentally using normal matter ions that are cheap to produce, relatively easy to handle, and provide a good indication of overall trap behavior (with the exception of assessing annihilation losses). The ions are produced via a positive hydrogen ion source and transported to HiPAT in a beam line equipped with electrostatic optics. The optics serve to both focus and gate the incoming ions, providing microsecond-timed beam pulses that are dynamically captured by cycling the HiPAT forward containment field like a "trap door". Initial dynamic capture experiments have been successfully performed with beam energy and currents set to 1.9 kV and 23 micro-amps, respectively. At these settings up to 2x10(exp 9) ions have been trapped during a single dynamic cycle.

  12. Diagnostic accuracy of PAT-POPS and ManChEWS for admissions of children from the emergency department

    PubMed Central

    Cotterill, Sarah; Rowland, Andrew G; Kelly, Jacqueline; Lees, Helen; Kamara, Mohammed

    2016-01-01

    Background The Pennine Acute Trust (PAT) Paediatric Observation Priority Score (PAT-POPS) is a specific emergency department (ED) physiological and observational aggregate scoring system, with scores of 0–18. A higher score indicates greater likelihood of admission. The Manchester Children's Early Warning System (ManChEWS) assesses six physiological observations to create a trigger score, classified as Green, Amber or Red. Methods Prospectively collected data were used to calculate PAT-POPS and ManChEWS on 2068 patients aged under 16 years (mean 5.6 years, SD 4.6) presenting over 1 month to a UK District General Hospital Paediatric ED. Receiver operating characteristics (ROC) comparison, using STATA V.13, was used to investigate the ability of ManChEWS and PAT-POPS to predict admission to hospital within 72 h of presentation to the ED. Results Comparison of the area under the ROC curve indicates that the ManChEWS ROC is 0.67 (95% CI 0.64 to 0.70) and the PAT-POPS ROC is 0.72 (95% CI 0.68 to 0.75). The difference is statistically significant. At a PAT-POPS cut-off of ≥2, 80% of patients had their admission risk correctly classified (positive likelihood ratio 3.40, 95% CI 2.90 to 3.98) whereas for ManChEWS with a cut off of ≥Amber only 71% of patients were correctly classified (positive likelihood ratio 2.18, 95% CI 1.94 to 2.45). Conclusions PAT-POPS is a more accurate predictor of admission risk than ManChEWS. Replacing ManChEWS with PAT-POPS would appear to be clinically appropriate in a paediatric ED. This needs validation in a multicentre study. PMID:27068865

  13. Ion Storage Tests with the High Performance Antimatter Trap (HiPAT)

    NASA Technical Reports Server (NTRS)

    Martin, James J.; Lewis, Raymond A.; Chakrabarti, Suman; Pearson, Boise; Schafer, Charles (Technical Monitor)

    2002-01-01

    The NASA/Marshall Space Flight Centers (NASA/MSFC) Propulsion Research Center (PRC) is evaluating an antiproton storage system, referred to as the High Performance Antiproton Trap (HiPAT). This interest stems from the sheer energy represented by matter/antimatter annihilation process with has an energy density approximately 10 order of magnitude above that of chemical propellants. In other terms, one gram of antiprotons contains the equivalent energy of approximately 23 space shuttle external tanks or ET's (each ET contains roughly 740,000 kgs of fuel and oxidizer). This incredible source of stored energy, if harnessed, would be an enabling technology for deep space mission where both spacecraft weight and propulsion performance are key to satisfying aggressive mission requirements. The HiPAT hardware consists of a 4 Tesla superconductor system, an ultra high vacuum test section (vacuum approaching 10(exp -12) torr), and a high voltage confinement electrode system (up to 20 kvolts operation). The current laboratory layout is illustrated. The HiPAT designed objectives included storage of up to 1 trillion antiprotons with corresponding lifetimes approaching 18 days. To date, testing has centered on the storage of positive hydrogen ions produced in situ by a stream of high-energy electrons that passes through the trapping region. However, due to space charge issues and electron beam compression as it passes through the HiPAT central field, current ion production is limited to less then 50,000 ions. Ion lifetime was determined by counting particle populations at the end of various storage time intervals. Particle detection was accomplished by destructively expelling the ions against a micro-channel plate located just outside the traps magnetic field. The effect of radio frequency (RF) stabilization on the lifetime of trapped particles was also examined. This technique, referred to as a rotating wall, made use of a segmented electrode located near the center of the trap

  14. Expression of PAT and NPT II proteins during the developmental stages of a genetically modified pepper developed in Korea.

    PubMed

    Kim, Hyo Jin; Lee, Si Myung; Kim, Jae Kwang; Ryu, Tae Hun; Suh, Seok Cheol; Cho, Hyun Suk

    2010-10-27

    Estimation of the protein levels introduced in a biotechnology-derived product is conducted as part of an overall safety assessment. An enzyme-linked immunosorbent assay (ELISA) was used to analyze phosphinothricin acetyltransferase (PAT) and neomycin phosphotransferase II (NPT II) protein expression in a genetically modified (GM) pepper plant developed in Korea. PAT and NPT II expression levels, based on both dry weight and fresh weight, were variable among different plant generations and plant sections from isolated genetically modified organism (GMO) fields at four developmental stages. PAT expression was highest in leaves at anthesis (11.44 μg/gdw and 2.17 μg/gfw) and lowest in roots (0.12 μg/gdw and 0.01 μg/gfw). NPT II expression was also highest in leaves at anthesis (17.31 μg/gdw and 3.41 μg/gfw) and lowest in red pepper (0.65 μg/gdw and 0.12 μg/gfw). In pollen, PAT expression was 0.59-0.62 μg/gdw, while NPT II was not detected. Both PAT and NPT II showed a general pattern of decreased expression with progression of the growing season. As expected, PAT and NPT II protein expression was not detectable in control pepper plants.

  15. The Lsm1-7-Pat1 complex promotes viral RNA translation and replication by differential mechanisms.

    PubMed

    Jungfleisch, Jennifer; Chowdhury, Ashis; Alves-Rodrigues, Isabel; Tharun, Sundaresan; Díez, Juana

    2015-08-01

    The Lsm1-7-Pat1 complex binds to the 3' end of cellular mRNAs and promotes 3' end protection and 5'-3' decay. Interestingly, this complex also specifically binds to cis-acting regulatory sequences of viral positive-strand RNA genomes promoting their translation and subsequent recruitment from translation to replication. Yet, how the Lsm1-7-Pat1 complex regulates these two processes remains elusive. Here, we show that Lsm1-7-Pat1 complex acts differentially in these processes. By using a collection of well-characterized lsm1 mutant alleles and a system that allows the replication of Brome mosaic virus (BMV) in yeast we show that the Lsm1-7-Pat1 complex integrity is essential for both, translation and recruitment. However, the intrinsic RNA-binding ability of the complex is only required for translation. Consistent with an RNA-binding-independent function of the Lsm1-7-Pat1 complex on BMV RNA recruitment, we show that the BMV 1a protein, the sole viral protein required for recruitment, interacts with this complex in an RNA-independent manner. Together, these results support a model wherein Lsm1-7-Pat1 complex binds consecutively to BMV RNA regulatory sequences and the 1a protein to promote viral RNA translation and later recruitment out of the host translation machinery to the viral replication complexes.

  16. Performance characterization of low-cost, high-speed, portable pulsed laser diode photoacoustic tomography (PLD-PAT) system.

    PubMed

    Upputuri, Paul Kumar; Pramanik, Manojit

    2015-10-01

    Photoacoustic tomography systems that uses Q-switched Nd:YAG/OPO pulsed lasers are expensive, bulky, and hence limits its use in clinical applications. The low pulse repetition rate of these lasers makes it unsuitable for real-time imaging when used with single-element ultrasound detector. In this work, we present a pulsed laser diode photoacoustic tomography (PLD-PAT) system that integrates a compact PLD inside a single-detector circular scanning geometry. We compared its performance against the traditional Nd:YAG/OPO based PAT system in terms of imaging depth, resolution, imaging time etc. The PLD provides near-infrared pulses at ~803 nm wavelength with pulse energy ~1.4 mJ/pulse at 7 kHz repetition rate. The PLD-PAT system is capable of providing 2D image in scan time as small as 3 sec with a signal-to-noise ratio ~30. High-speed and deep-tissue imaging is demonstrated on phantoms and biological samples. The PLD-PAT system is inexpensive, portable, allows high-speed PAT imaging, and its performance is as good as traditional expensive OPO based PAT system. Therefore, it holds promises for future translational biomedical imaging applications.

  17. Transcriptomic analysis of tomato carpel development reveals alterations in ethylene and gibberellin synthesis during pat3/pat4 parthenocarpic fruit set

    PubMed Central

    Pascual, Laura; Blanca, Jose M; Cañizares, Joaquin; Nuez, Fernado

    2009-01-01

    Background Tomato fruit set is a key process that has a great economic impact on crop production. We employed the Affymetrix GeneChip Tomato Genome Array to compare the transcriptome of a non-parthenocarpic line, UC82, with that of the parthenocarpic line RP75/59 (pat3/pat4 mutant). We analyzed the transcriptome under normal conditions as well as with forced parthenocarpic development in RP75/59, emasculating the flowers 2 days before anthesis. This analysis helps to understand the fruit set in tomato. Results Differentially expressed genes were extracted with maSigPro, which is designed for the analysis of single and multiseries time course microarray experiments. 2842 genes showed changes throughout normal carpel development and fruit set. Most of them showed a change of expression at or after anthesis. The main differences between lines were concentrated at the anthesis stage. We found 758 genes differentially expressed in parthenocarpic fruit set. Among these genes we detected cell cycle-related genes that were still activated at anthesis in the parthenocarpic line, which shows the lack of arrest in the parthenocarpic line at anthesis. Key genes for the synthesis of gibberellins and ethylene, which were up-regulated in the parthenocarpic line were also detected. Conclusion Comparisons between array experiments determined that anthesis was the most different stage and the key point at which most of the genes were modulated. In the parthenocarpic line, anthesis seemed to be a short transitional stage to fruit set. In this line, the high GAs contends leads to the development of a parthenocarpic fruit, and ethylene may mimic pollination signals, inducing auxin synthesis in the ovary and the development of a jelly fruit. PMID:19480705

  18. 100th anniversary of the death of Ricketts: Howard Taylor Ricketts (1871-1910). The namesake of the Rickettsiaceae family.

    PubMed

    Gross, Dominik; Schäfer, Gereon

    2011-01-01

    The US American pathologist and microbiologist Howard Taylor Ricketts died 100 years ago. He is renowned for discovering the causative organism and the transmission route of Rocky Mountain spotted fever and of tabardillo--an epidemic louse-borne typhus occurring especially in Mexico. He also found that both diseases were caused by related infectious agents (Rickettsia rickettsii and Rickettsia prowazekii). The scientific community therefore named both a taxonomic family (Rickettsiaceae) and an order (Rickettsiales) after the scientist. Ricketts' work on immunity and serums became the basis for further advances in vaccine development.

  19. Social contract of academic medical centres to the community: Dr Howard Atwood Kelly (1858-1943), a historical perspective.

    PubMed

    Allen, Paul

    2016-05-01

    Academic medical centres have traditionally been bastions of teaching and research. Outreach to the community at large and involvement in community affairs have sometimes been lacking in the overall mission and activities of academic medical centres. This paper provides an historical perspective first on the numerous achievements of a physician and surgeon and then on the topic of involvement in community affairs by reviewing the many contributions of America's pioneer gynaecological surgeon and one of the four physician founders of the Johns Hopkins Hospital and School of Medicine in 1889 - Dr Howard Atwood Kelly.

  20. A micromachined silicon parallel acoustic delay line (PADL) array for real-time photoacoustic tomography (PAT)

    NASA Astrophysics Data System (ADS)

    Cho, Young Y.; Chang, Cheng-Chung; Wang, Lihong V.; Zou, Jun

    2015-03-01

    To achieve real-time photoacoustic tomography (PAT), massive transducer arrays and data acquisition (DAQ) electronics are needed to receive the PA signals simultaneously, which results in complex and high-cost ultrasound receiver systems. To address this issue, we have developed a new PA data acquisition approach using acoustic time delay. Optical fibers were used as parallel acoustic delay lines (PADLs) to create different time delays in multiple channels of PA signals. This makes the PA signals reach a single-element transducer at different times. As a result, they can be properly received by single-channel DAQ electronics. However, due to their small diameter and fragility, using optical fiber as acoustic delay lines poses a number of challenges in the design, construction and packaging of the PADLs, thereby limiting their performances and use in real imaging applications. In this paper, we report the development of new silicon PADLs, which are directly made from silicon wafers using advanced micromachining technologies. The silicon PADLs have very low acoustic attenuation and distortion. A linear array of 16 silicon PADLs were assembled into a handheld package with one common input port and one common output port. To demonstrate its real-time PAT capability, the silicon PADL array (with its output port interfaced with a single-element transducer) was used to receive 16 channels of PA signals simultaneously from a tissue-mimicking optical phantom sample. The reconstructed PA image matches well with the imaging target. Therefore, the silicon PADL array can provide a 16× reduction in the ultrasound DAQ channels for real-time PAT.

  1. Community-based parenteral anti-infective therapy (CoPAT). Pharmacokinetic and monitoring issues.

    PubMed

    Williams, D N; Raymond, J L

    1998-07-01

    Community-based parenteral anti-infective therapy (CoPAT) has, over the past 20 years, increased rapidly in many parts of the world including North America, Europe, South America and Australia. CoPAT is a multidisciplinary activity demanding close cooperation between nurses, pharmacists and physicians, as well as with the patient. The selection of an anti-infective drug for use outside the hospital setting must take into account not only the therapeutic effectiveness, cost effectiveness and safety of the drug, but also pharmacological factors such as the dosage schedule and the stability of the drug. Dosage schedules vary with pharmacokinetic factors (e.g. the use of drugs with long half-lives are favoured by CoPAT programmes) and pharmacodynamic features (e.g. once daily gentamicin therapy is attractive and practical because of concentration-dependent bactericidal killing and prolonged post-antibiotic effect). With selected drugs, the renal and, to a lesser degree, liver function of the patient will influence the dosage schedule. The mode of intravenous (i.v.) drugs administration will vary with volume considerations (limiting the use of syringe-infusion therapy for some drugs), stability issues (prevents drugs that are stable at room temperature for less than 24 hours from being used in multidose computerised delivery systems), as well as patient factors (ability to self administer an i.v. drug). Monitoring serum anti-microbial concentrations is undertaken to assure effectiveness and avoid toxicity, and is indicated for drugs with a narrow therapeutic window, such as the aminoglycosides. With the advent of the single daily dose administration of aminoglycosides, checking serum concentrations at the mid-point, i.e. 6 to 14 hours following administration of the first dose, is one approach. Because the toxic effects of vancomycin have been overstated, serum concentrations should only be obtained for defined indications primarily to assure therapeutic effectiveness.

  2. Dispersion and Transport of Cryptosporidium Oocysts from Fecal Pats under Simulated Rainfall Events

    PubMed Central

    Davies, Cheryl M.; Ferguson, Christobel M.; Kaucner, Christine; Krogh, Martin; Altavilla, Nanda; Deere, Daniel A.; Ashbolt, Nicholas J.

    2004-01-01

    The dispersion and initial transport of Cryptosporidium oocysts from fecal pats were investigated during artificial rainfall events on intact soil blocks (1,500 by 900 by 300 mm). Rainfall events of 55 mm h−1 for 30 min and 25 mm h−1 for 180 min were applied to soil plots with artificial fecal pats seeded with approximately 107 oocysts. The soil plots were divided in two, with one side devoid of vegetation and the other left with natural vegetation cover. Each combination of event intensity and duration, vegetation status, and degree of slope (5° and 10°) was evaluated twice. Generally, a fivefold increase (P < 0.05) in runoff volume was generated on bare soil compared to vegetated soil, and significantly more infiltration, although highly variable, occurred through the vegetated soil blocks (P < 0.05). Runoff volume, event conditions (intensity and duration), vegetation status, degree of slope, and their interactions significantly affected the load of oocysts in the runoff. Surface runoff transported from 100.2 oocysts from vegetated loam soil (25-mm h−1, 180-min event on 10° slope) to up to 104.5 oocysts from unvegetated soil (55-mm h−1, 30-min event on 10° slope) over a 1-m distance. Surface soil samples downhill of the fecal pat contained significantly higher concentrations of oocysts on devegetated blocks than on vegetated blocks. Based on these results, there is a need to account for surface soil vegetation coverage as well as slope and rainfall runoff in future assessments of Cryptosporidium transport and when managing pathogen loads from stock grazing near streams within drinking water watersheds. PMID:14766600

  3. EARLY IMPACT MELTING AND SPACE EXPOSURE HISTORY OF THE PAT91501 LCHONDRITE

    NASA Technical Reports Server (NTRS)

    Bogard, Donald D.; Garrison, D. H.; Herzog, G. F.; Xue, S.; Klein, J.; Middleton, R.

    2004-01-01

    Collisions probably occurred frequently in the early history of the asteroid belt. Their effects, which should be recorded in meteorites, must have included heating and melting along with shock alteration of mineral textures. Some non-chondritic meteorite types e.g., eucrites and IIE and IAB irons - do indeed give evidence of extensive impact heating more than 3.4 Gyr ago. The ordinary chondrites, in contrast, show little evidence of early impact heating. The Ar-Ar and Rb-Sr ages of ordinary chondrites that experienced intense shock are for the most part relatively young, many less than 1.5 Gyr. The numerous L-chondrites with Ar- Ar ages clustering near 0.5 Gy are a well-known example. One of them, the 105-kg Chico Lchondrite, shows the effects of unusually intense heating. It is approximately 60% impact melt and likely formed as a dyke beneath a large crater when the L-chondrite parent body underwent a very large impact approximately 0.5 Gyr ago. In rare instances, older shock dates are indicated for ordinary chondrites. Dixon et al show early impact resetting of Ar-Ar ages of a few LL-chondrites including MIL 99301 at 4.23 0.03 Gyr, but in none of these stones did shock lead to extensive melting. As of 2003, searches for chondritic melts attributable to early shock had turned up only the Shaw L-chondrite, which has an Ar-Ar age of approximately 4.42 Gyr. PAT91501 is an 8.55-kg L-chondrite containing vesicles and metal-troilite nodules. It is a unique, near-total impact melt, unshocked, depleted in siderophile and chalcophile elements, and contains only approximately 10% relic chondritic material. The authors conclude that PAT91501 crystallized rapidly and from a much more homogeneous melt than did Shaw. They suggest that PAT resembles Chico and likely formed as an impact melt vein within an impact crater. To define the history of PAT, we have determined its Ar-39-Ar-40 age and measured several radioactive and stable nuclides produced during its space exposure to

  4. Howard I. Maibach: extraordinary leadership in integrating key concepts underpinning our understanding of percutaneous absorption and occupational dermatology.

    PubMed

    Surber, C; Elsner, P; Singh, J

    2013-01-01

    The purpose of the present article is to briefly highlight some contributions of Prof. Howard I. Maibach to the field of dermatology. After a few introducing remarks regarding Howard's personal career, the article specifically reviews contributions to the understanding of percutaneous absorption and to occupational dermatology. He and his companions/coworkers established and introduced experimental prerequisites to better study and understand percutaneous absorption - both in vitro and in vivo. Not less influential was his contribution to occupational dermatology acting as a founding member of the International Contact Dermatitis Research Group and coinaugurating the North American Contact Dermatitis Group. These groups have been very active ever since. As an academic teacher, he inspired young colleagues to perform original research work and to establish their own working groups. He has done this most successfully with many fellows who worked with him over the years, and who are now leading departments or companies dedicated to dermatological research all over the world. Probably this is his most important and lasting achievement.

  5. HetF and PatA control levels of HetR in Anabaena sp. strain PCC 7120.

    PubMed

    Risser, Douglas D; Callahan, Sean M

    2008-12-01

    Anabaena sp. strain PCC 7120 is a filamentous cyanobacterium that differentiates heterocysts in response to deprivation of combined nitrogen. A hetF deletion strain lacked heterocysts and had aberrant cell morphology. Site-directed mutagenesis of the predicted active-site histidine and cysteine residues of this putative caspase-hemoglobinase fold protease abolished HetF function, supporting the hypothesis that HetF is a protease. Deletion of patA, which is necessary for the formation of most intercalary heterocysts, or hetF resulted in an increase in HetR protein, and extra copies of hetF on a plasmid functionally bypassed the deletion of patA. A hetR-gfp translational fusion expressed from an inducible promoter demonstrated that hetF-dependent downregulation of HetR levels occurs rapidly in vegetative cells, as well as developing heterocysts. "Mosaic" filaments in which only one cell of a filament had a copy of hetR or hetF indicated that hetF is required for differentiation only in cells that will become heterocysts. hetF was required for transcription from a hetR-dependent transcription start point of the hetR promoter and induction of transcription from the patS promoter. The inverse correlation between the level of HetR protein and transcription from hetR-dependent promoters suggests that the transcriptional activity of HetR is regulated by HetF and PatA.

  6. Bridging the gap between PAT concepts and implementation: An integrated software platform for fermentation.

    PubMed

    Chopda, Viki R; Gomes, James; Rathore, Anurag S

    2016-01-01

    Bioreactor control significantly impacts both the amount and quality of the product being manufactured. The complexity of the control strategy that is implemented increases with reactor size, which may vary from thousands to tens of thousands of litres in commercial manufacturing. The Process Analytical Technology (PAT) initiative has highlighted the need for having robust monitoring tools and effective control schemes that are capable of taking real time information about the critical quality attributes (CQA) and the critical process parameters (CPP) and executing immediate response as soon as a deviation occurs. However, the limited flexibility that present commercial software packages offer creates a hurdle. Visual programming environments have gradually emerged as potential alternatives to the available text based languages. This paper showcases development of an integrated programme using a visual programming environment for a Sartorius BIOSTAT® B Plus 5L bioreactor through which various peripheral devices are interfaced. The proposed programme facilitates real-time access to data and allows for execution of control actions to follow the desired trajectory. Major benefits of such integrated software system include: (i) improved real time monitoring and control; (ii) reduced variability; (iii) improved performance; (iv) reduced operator-training time; (v) enhanced knowledge management; and (vi) easier PAT implementation.

  7. The structure of the cyanobactin domain of unknown function from PatG in the patellamide gene cluster

    PubMed Central

    Mann, Greg; Koehnke, Jesko; Bent, Andrew F.; Graham, Rachael; Houssen, Wael; Jaspars, Marcel; Schwarz-Linek, Uli; Naismith, James H.

    2014-01-01

    Patellamides are members of the cyanobactin family of ribosomally synthesized and post-translationally modified cyclic peptide natural products, many of which, including some patellamides, are biologically active. A detailed mechanistic understanding of the biosynthetic pathway would enable the construction of a biotechnological ‘toolkit’ to make novel analogues of patellamides that are not found in nature. All but two of the protein domains involved in patellamide biosynthesis have been characterized. The two domains of unknown function (DUFs) are homologous to each other and are found at the C-termini of the multi-domain proteins PatA and PatG. The domain sequence is found in all cyanobactin-biosynthetic pathways characterized to date, implying a functional role in cyanobactin biosynthesis. Here, the crystal structure of the PatG DUF domain is reported and its binding interactions with plausible substrates are investigated. PMID:25484206

  8. Building on and Honoring Forty Years of PBL Scholarship from Howard Barrows: A Scientometric, Large-Scale Data, and Visualization-Based Analysis

    ERIC Educational Resources Information Center

    Xian, Hanjun; Madhavan, Krishna

    2013-01-01

    Over the past forty years, Howard Barrows' contributions to PBL research have influenced and guided educational research and practice in a diversity of domains. It is necessary to make visible to all PBL scholars what has been accomplished, what is perceived as significant, and what is the scope of applicability for Barrows' groundbreaking…

  9. Why Race and Culture Matter in Schools, and Why We Need to "Get This Right": A Conversation with Dr. Tyrone Howard

    ERIC Educational Resources Information Center

    Clark, Pat; Zygmunt, Eva; Howard, Tyrone

    2016-01-01

    Tyrone Howard is Professor of Education at UCLA; Associate Dean of Equity, Diversity, and Inclusion; and former Director of Center X, which is where UCLA's teacher education program is housed. Center X provides a unique setting where researchers and practitioners collaborate to design and conduct programs that prepare and support K-12 teachers and…

  10. "Sophisticated Pedagogical Underpinnings"? A Response to Roper and Davis's "Howard Gardner: Knowledge, Learning and Development in Drama and Arts Education" (RIDE, 5[2]).

    ERIC Educational Resources Information Center

    Saxton, Juliana; Miller, Carole

    2001-01-01

    Responds to an article in the previous issue of this journal discussing Howard Gardner's work and its relation to drama education. Suggests that when Gardner's multiple intelligences theory is applied in teaching, differences are valued and communities are strengthened. Concludes that Gardner's theory helps to validate the practice and place of…

  11. The Howard Gardner School: A Portrait of a Small Progressive, Independent and Alternative High School for Bright, Creative Non-Traditional Learners

    ERIC Educational Resources Information Center

    Drakeford, Jocelyn G.

    2010-01-01

    Howard Gardner's theory of multiple intelligences has impacted the educational community since its inception in 1983. The theory became the framework for Gifted and Talented Education, in that it broaden the scope of giftedness to be defined in the context of students being identified with nontraditional gifts and talents as well as combinations…

  12. How Did Howard University, One of the Leading Historically Black Colleges and Universities, Develop as an Academic Institution during the Period 1967 through 1997?

    ERIC Educational Resources Information Center

    Williams, Sharon

    This paper reviews the Development of Howard University (District of Columbia) during the period 1967-1997 as the institution adapted to broad social, cultural, and intellectual changes shaping the country during this period. The study's conceptual framework followed Mysers' theory of the six stages of the education of Blacks, especially the…

  13. The Psychosocial Assessment Tool (PAT2.0): Psychometric Properties of a Screener for Psychosocial Distress in Families of Children Newly Diagnosed with Cancer

    PubMed Central

    Pai, Ahna L. H.; Patiño-Fernández, Anna Maria; McSherry, Mary; Beele, David; Alderfer, Melissa A.; Reilly, Anne T.; Hwang, Wei-Ting; Kazak, Anne E.

    2010-01-01

    Purpose Psychometric properties of the Psychosocial Assessment Tool 2.0 (PAT2.0), a brief screener for psychosocial risk in families of children with cancer, are presented. Methods Female (N =132) and male (N =72) caregivers of 141 children newly diagnosed with cancer completed the PAT2.0 and measures of child behavior symptoms, anxiety, acute stress, and family functioning to establish validity. Internal consistency and test–retest reliability of the PAT2.0 were also examined. Results Internal consistency and two-week test–retest for the PAT2.0 Total score was strong. Validity for the PAT2.0 was supported by significant correlations between the PAT2.0 subscales and measures of corresponding constructs. PAT2.0 Total scores were correlated with acute stress and child behavior symptoms for both mothers and fathers. Receiver-Operating Characteristic curves provided preliminary support for the proposed cutoffs. Conclusion The PAT2.0 Total score is a useful screening tool for family psychosocial risk in the pediatric oncology population. PMID:17609228

  14. "Anything That Gets Me in My Heart": Pat Parker's Poetry of Justice.

    PubMed

    Green, David B

    2015-01-01

    This article argues that love and justice are interlocking themes that undergird and motivate the poetry and activism of the Black lesbian feminist Pat Parker. Parker was a prolific working-class poet, a committed Black lesbian feminist, and an international trailblazer whose poems, like her famous "Womanslaughter" discussed in this article, document the many injustices that Black women endured in an anti-Black, rabidly homophobic, and patriarchal U.S. during the last decades of the twentieth century. In a political moment where righteous cries of #BlackLivesMatter are heard across the United States I use this article to remind us all of the historical importance that Black lesbians played and continue to play in the struggles of anti-racist justice in America.

  15. PAT: From Western solid dosage forms to Chinese materia medica preparations using NIR-CI.

    PubMed

    Zhou, Luwei; Xu, Manfei; Wu, Zhisheng; Shi, Xinyuan; Qiao, Yanjiang

    2016-01-01

    Near-infrared chemical imaging (NIR-CI) is an emerging technology that combines traditional near-infrared spectroscopy with chemical imaging. Therefore, NIR-CI can extract spectral information from pharmaceutical products and simultaneously visualize the spatial distribution of chemical components. The rapid and non-destructive features of NIR-CI make it an attractive process analytical technology (PAT) for identifying and monitoring critical control parameters during the pharmaceutical manufacturing process. This review mainly focuses on the pharmaceutical applications of NIR-CI in each unit operation during the manufacturing processes, from the Western solid dosage forms to the Chinese materia medica preparations. Finally, future applications of chemical imaging in the pharmaceutical industry are discussed.

  16. Reduced-size plutonium sample processing and packaging for the PAT-2 package

    SciTech Connect

    Kuhn, E.; Deron, S.; Aigner, H.; Andersen, J.A.

    1982-01-01

    A light-water container for the air transport of plutonium safeguards samples, the PAT-2 package, has been developed in the USA and is now licensed by the US NRC (Certificate of Compliance) and the US DOT (IAEA Certificate of Competent Authority). The very limited available space in this package for plutonium-bearing samples required the design of small-size canisters to meet the needs of international safeguards. The suitability of a new small canister and vial for powder and solution samples has been tested in an intralaboratory experiment. The results of the experiment, based on the concept of pre-weighed samples, show that the tested canister and quartz vial can be used successfully for containing small size PuO/sub 2/ powder samples of homogeneous source material, as well as for dried aliguands of plutonium nitrate solutions.

  17. RF Manipulation of Ions in the High Performance Antiproton Trap (HiPAT)

    NASA Technical Reports Server (NTRS)

    Pearson, J. Boise; Martin, James J.; Sims, William H.; Chakrabarti, Suman; Lewis, Raymond A.; Rodgers, Stephen L. (Technical Monitor)

    2002-01-01

    The annihilation of antimatter provides the highest mass specific energy of any other known reaction. Proper harnessing of this energy holds great promise for future space propulsion systems. Many different propulsion concepts have been proposed that take advantage of antimatter, either using matter-antimatter as the primary fuel, or as a 'spark plug' for fusion and fission systems. In order to begin to address these concepts experimentally, a method of storing and transporting antimatter must be developed. The High Performance Antiproton Trap (HiPAT) is a first-generation storage and transportation device designed to store and transport 10(exp 12) antiprotons with a storage half-life of 18 days. It uses a Penning-Malmberg ion trap with a 4T magnetic field and 20 kV potential. This will enable researchers much more flexibility in the design of antimatter experiments related to propulsion. Ions cannot be stored indefinitely in a real trap, as ion cloud instabilities develop from imperfections in manufacturing and misalignments in assembly. Previous work has been done at both the National Institute of Standards and University of California in San Diego in using RF (radio frequency) signals to both diagnose and confine the ion cloud. Two electrodes in the trap have been segmented to allow both reception and transmission of RF waves in the ion cloud. Experiments are underway to determine the number of ions and density in the cloud by "listening" to protons contained in the HiPAT. Currently we believe the density of ions stored in the trap is roughly 10(exp 15) m(exp -3). Development of non-destructive techniques is vital to the project goals, enabling continuous monitoring of the quantities stored in the system. Experimental work is also being done in identifying RF transmission frequencies that can manipulate the density of the cloud, by exchanging energy and momentum between the RF wave and the ions. Preliminary experiments have demonstrated this interaction.

  18. Hydraulic conductivity and PAT determine hierarchical resource partitioning and ramet development along Fragaria stolons.

    PubMed

    Atkinson, Christopher J; Else, Mark A

    2012-09-01

    Co-ordination of metabolic and physiological activity between plant parts is key to the control of growth and development. Here the movement of resources and their allocation between mother plants and daughter ramets along Fragaria stolons was quantified with respect to hierarchy. Gradients of internodal ramet leaf water potential (ψ) and stolon and ramet hydraulic conductivities (L) were measured together with apparent stolon IAA movement via the polar auxin transport pathway (PAT). These processes are linked with measurements of stolon vascular development. The pattern of tissue differentiation and lignification in sequential stele sections of stolons demonstrated the rapid acquisition of the capacity for water transport, with transpiration potentially varying systematically with stolon lignification and the acropetal decline in stolon xylem ψ. Stolon and ramet L declined acropetally, with L across older ramets being significantly lower than that of the connecting stolons. The capacity for polar IAA transport increased with stolon age; this was due to increased transport intensity in older tissues. The partitioning of dry matter was strongly hierarchical with younger ramets smaller than older ramets, while foliar concentrations of N, P, and K were greater for the younger ramets. The results show that stolon anatomy develops rapidly at the apical end, facilitating hierarchical ramet development, which is evident as a basipetal increase in L. The rapid development of transport tissue functionality enables young unrooted ramets to acquire water, in order to supply an expanding leaf area, as well as mineral ions disproportionally with respect to older ramets. This facilitates colonization and self-rooting of apical ramets. The unidirectional increase in basipetal PAT along stolons facilitates hierarchical ramet development.

  19. mini-PAT (Peer Assessment Tool): A Valid Component of a National Assessment Programme in the UK?

    ERIC Educational Resources Information Center

    Archer, Julian; Norcini, John; Southgate, Lesley; Heard, Shelley; Davies, Helena

    2008-01-01

    Purpose: To design, implement and evaluate a multisource feedback instrument to assess Foundation trainees across the UK. Methods: mini-PAT (Peer Assessment Tool) was modified from SPRAT (Sheffield Peer Review Assessment Tool), an established multisource feedback (360 [degree]) instrument to assess more senior doctors, as part of a blueprinting…

  20. Simultaneous binding of the anti-cancer IgM monoclonal antibody PAT-SM6 to low density lipoproteins and GRP78.

    PubMed

    Rosenes, Zachary; Mok, Yee-Foong; Yang, Shuo; Griffin, Michael D W; Mulhern, Terrence D; Hatters, Danny M; Hensel, Frank; Howlett, Geoffrey J

    2013-01-01

    The tumour-derived monoclonal IgM antibody PAT-SM6 specifically kills malignant cells by an apoptotic mechanism linked to the excessive uptake of plasma lipids. The mechanism is postulated to occur via the multi-point attachment of PAT-SM6 to the unfolded protein response regulator GRP78, located on the surface of tumour cells, coupled to the simultaneous binding of plasma low density lipoprotein (LDL). We prepared and characterised LDL and oxidized LDL using sedimentation velocity and small-angle X-ray scattering (SAXS) analysis. Enzyme-linked immunosorbent (ELISA) techniques indicated apparent dissociation constants of approximately 20 nM for the binding of LDL or oxidized LDL to PAT-SM6. ELISA experiments showed cross competition with LDL inhibiting PAT-SM6 binding to immobilised GRP78, while, in the reverse experiment, GRP78 inhibited PAT-SM6 binding to immobilized LDL. In contrast to the results of the ELISA experiments, sedimentation velocity experiments indicated relatively weak interactions between LDL and PAT-SM6, suggesting immunoabsorbance to the microtiter plate is driven by an avidity-based binding mechanism. The importance of avidity and the multipoint attachment of antigens to PAT-SM6 was further investigated using antigen-coated polystyrene beads. Absorption of GRP78 or LDL to polystyrene microspheres led to an increase in the inhibition of PAT-SM6 binding to microtiter plates coated with GRP78 or LDL, respectively. These results support the hypothesis that the biological action of PAT-SM6 in tumour cell apoptosis depends on the multivalent nature of PAT-SM6 and the ability to interact simultaneously with LDL and multiple GRP78 molecules clustered on the tumour cell surface.

  1. P-body components, Dhh1 and Pat1, are involved in tRNA nuclear-cytoplasmic dynamics

    PubMed Central

    Hurto, Rebecca L.; Hopper, Anita K.

    2011-01-01

    The nuclear-cytoplasmic distribution of tRNA depends on the balance between tRNA nuclear export/re-export and retrograde tRNA nuclear import in Saccharomyces cerevisiae. The distribution of tRNA is sensitive to nutrient availability as cells deprived of various nutrients exhibit tRNA nuclear accumulation. Starvation induces numerous events that result in translational repression and P-body formation. This study investigated the possible coordination of these responses with tRNA nuclear-cytoplasmic distribution. Dhh1 and Pat1 function in parallel to promote translation repression and P-body formation in response to starvation. Loss of both, Dhh1 and Pat1, results in a failure to repress translation and to induce P-body formation in response to glucose starvation. This study reports that nutrient deprived dhh1 pat1 cells also fail to accumulate tRNA within nuclei. Conversely, inhibition of translation initiation and induction of P-body formation by overproduction of Dhh1 or Pat1 cause tRNA nuclear accumulation in nutrient-replete conditions. Also, loss of the mRNA decapping activator, Lsm1, causes tRNA nuclear accumulation. However, the coordination between P-body formation, translation repression, and tRNA distribution is limited to the early part of the P-body formation/translation repression pathway as loss of mRNA decapping or 5′ to 3′ degradation does not influence tRNA nuclear-cytoplasmic dynamics. The data provide the first link between P-body formation/translation initiation and tRNA nuclear-cytoplasmic dynamics. The current model is that Dhh1 and Pat1 function in parallel to promote starvation-induced tRNA nuclear accumulation. PMID:21398402

  2. BioPatRec: A modular research platform for the control of artificial limbs based on pattern recognition algorithms

    PubMed Central

    2013-01-01

    Background Processing and pattern recognition of myoelectric signals have been at the core of prosthetic control research in the last decade. Although most studies agree on reporting the accuracy of predicting predefined movements, there is a significant amount of study-dependent variables that make high-resolution inter-study comparison practically impossible. As an effort to provide a common research platform for the development and evaluation of algorithms in prosthetic control, we introduce BioPatRec as open source software. BioPatRec allows a seamless implementation of a variety of algorithms in the fields of (1) Signal processing; (2) Feature selection and extraction; (3) Pattern recognition; and, (4) Real-time control. Furthermore, since the platform is highly modular and customizable, researchers from different fields can seamlessly benchmark their algorithms by applying them in prosthetic control, without necessarily knowing how to obtain and process bioelectric signals, or how to produce and evaluate physically meaningful outputs. Results BioPatRec is demonstrated in this study by the implementation of a relatively new pattern recognition algorithm, namely Regulatory Feedback Networks (RFN). RFN produced comparable results to those of more sophisticated classifiers such as Linear Discriminant Analysis and Multi-Layer Perceptron. BioPatRec is released with these 3 fundamentally different classifiers, as well as all the necessary routines for the myoelectric control of a virtual hand; from data acquisition to real-time evaluations. All the required instructions for use and development are provided in the online project hosting platform, which includes issue tracking and an extensive “wiki”. This transparent implementation aims to facilitate collaboration and speed up utilization. Moreover, BioPatRec provides a publicly available repository of myoelectric signals that allow algorithms benchmarking on common data sets. This is particularly useful for

  3. Principles and Practices Fostering Inclusive Excellence: Lessons from the Howard Hughes Medical Institute’s Capstone Institutions

    PubMed Central

    DiBartolo, Patricia Marten; Gregg-Jolly, Leslie; Gross, Deborah; Manduca, Cathryn A.; Iverson, Ellen; Cooke, David B.; Davis, Gregory K.; Davidson, Cameron; Hertz, Paul E.; Hibbard, Lisa; Ireland, Shubha K.; Mader, Catherine; Pai, Aditi; Raps, Shirley; Siwicki, Kathleen; Swartz, Jim E.

    2016-01-01

    Best-practices pedagogy in science, technology, engineering, and mathematics (STEM) aims for inclusive excellence that fosters student persistence. This paper describes principles of inclusivity across 11 primarily undergraduate institutions designated as Capstone Awardees in Howard Hughes Medical Institute’s (HHMI) 2012 competition. The Capstones represent a range of institutional missions, student profiles, and geographical locations. Each successfully directed activities toward persistence of STEM students, especially those from traditionally underrepresented groups, through a set of common elements: mentoring programs to build community; research experiences to strengthen scientific skill/identity; attention to quantitative skills; and outreach/bridge programs to broaden the student pool. This paper grounds these program elements in learning theory, emphasizing their essential principles with examples of how they were implemented within institutional contexts. We also describe common assessment approaches that in many cases informed programming and created traction for stakeholder buy-in. The lessons learned from our shared experiences in pursuit of inclusive excellence, including the resources housed on our companion website, can inform others’ efforts to increase access to and persistence in STEM in higher education. PMID:27562960

  4. Alpha synchronization as a brain model for unconscious defense: An overview of the work of Howard Shevrin and his team.

    PubMed

    Bazan, Ariane

    2017-03-01

    Howard Shevrin and his team have developed a stringent subliminal priming methodology, which experimentally approximates a situation of an internal, mental triggering of unconscious defense. Through a series of four studies they thus are able to bring evidence for this type of unconscious defense. With event-related potentials, three clinical studies show how synchronization of a specific brain wave, the alpha wave, known for its inhibitory function, is also induced by subliminally presented conflictual subject-specific stimuli. Therefore, alpha synchronization could serve as the brain mechanism of unconscious defense. The results only make sense if we suppose the existence of a dynamic unconscious, which has inherited childhood conflicts, and with privileged connections to neurotic symptom characteristics. Moreover, by showing that the unconscious conflict phrases, inferred by clinicians from clinical interviews, have a similar brain behavior, Shevrin and his team provide evidence that these inferences are not simply clinician-dependent subjective interpretations but also imply some form of independent mental reality. Finally, interpretation of the results has led us to propose two distinct physiological mechanisms for defense: one, unconscious defense, by alpha synchronization in connection with the drive derivatives, and another, repression, based on the indications of reality in connection with the ego.

  5. Effects of temperature and packaging types on ergosterol and Howard mold count values of tomato paste during storage.

    PubMed

    Ekinci, Raci; Kadakal, Çetin; Otağ, Mustafa

    2014-03-01

    The objective of the present study was to investigate the effects of temperature and packaging on ergosterol and Howard mold count (HMC) changes of tomato paste during storage. The other purpose of this study was to determine whether the measurement of ergosterol stability in tomato paste can be useful for the assessment of microbiological quality of tomato paste as related to the storage temperature (4, 20, 28, or 37°C) and time. Ergosterol analysis was done by using high-performance liquid chromatography. Tomato paste samples were packaged in either aseptic bags or tin boxes and stored at 4, 20, 28, or 37°C for a period of 10 months. The detection limit of ergosterol was 0.1 mg/kg. Measurements showed that packaging and storage temperatures of 28 and 37°C have a considerable influence on ergosterol and HMC changes in the product. The poor precision of the "percentage of discarded fruits" and HMC methods has increased the importance of ergosterol for the microbiological quality evaluation of tomato and tomato products. This article reports the data from what we believe to be the first survey for the influence of storage temperature and packaging material on ergosterol and HMC changes of tomato paste during storage.

  6. Health-hazard evaluation report HETA-88-311-0000, Office of Disability Determination, The Howard Building, Tampa, Florida

    SciTech Connect

    Roper, P.; Lushniak, B.

    1989-01-01

    In response to a request from the Area Manager of the Office of Disability Determination, an evaluation was made of possible hazardous working conditions at the Howard Building, Tampa, Florida. Particular concern was voiced with regard to eye irritation, headaches, and fatigue with a possible connection to water leakage into the offices and pigeon nesting areas above suspended ceilings. Studies indicated no carbon-monoxide, nitrogen-dioxide, or formaldehyde concentration in the building. Carbon dioxide levels of 1000 parts per million or above were recorded which were indicative of inadequate air exchange between a building and the outdoors. An area was found along the south wall of the building with gross contamination by bird droppings and other debris. Rainwater leakage above the areas where the birds had nested washed the excrement and debris down over the office walls. As much as 2 inches of bird droppings were found on some suspended tiles. The crawl space above the first floor was observed to have health and safety hazards. Some of the symptoms were likely allergic rhinitis while others may have resulted from a lack of fresh outdoor air. Specific measures should be taken to safely remove the bird debris, prevent additional bird inhabitation, improve drainage of condensate water, and provide an outdoor air-supply system.

  7. Inline monitoring and a PAT strategy for pharmaceutical hot melt extrusion.

    PubMed

    Wahl, Patrick R; Treffer, Daniel; Mohr, Stefan; Roblegg, Eva; Koscher, Gerold; Khinast, Johannes G

    2013-10-15

    Implementation of continuous manufacturing in the pharmaceutical industry requires tight process control. This study focuses on a PAT strategy for hot melt extrusion of vegetable calcium stearate (CaSt) as matrix carrier and paracetamol as active pharmaceutical ingredient (API). The extrusion was monitored using in-line near-infrared (NIR) spectroscopy. A NIR probe was located in the section between the extrusion screws and the die, using a novel design of the die channel. A chemometric model was developed based on premixes at defined concentrations and was implemented in SIPAT for real time API concentration monitoring. Subsequently, step experiments were performed for different API concentrations, screw speeds and screw designs. The predicted API concentration was in good agreement with the pre-set concentrations. The transition from one API plateau to another was a smooth curve due to the mixing behaviour of the extruder. The accuracy of the model was confirmed via offline HPLC analysis. The screw design was determined as the main influential factor on content uniformity (CU). Additionally the influence of multiple feeders had a significant impact on CU. The results demonstrate that in-line NIR measurements is a powerful tool for process development (e.g., mixing characterization), monitoring and further control strategies.

  8. Ion Storage with the High Performance Antiproton Trap (HiPAT)

    NASA Technical Reports Server (NTRS)

    Martin, James; Lewis, Raymond; Chakrabarti, Suman; Pearson, Boise

    2002-01-01

    The matter antimatter reaction represents the densest form of energy storage/release known to modern physics: as such it offers one of the most compact sources of power for future deep space exploration. To take the first steps along this path, NASA-Marshall Space Flight Center is developing a storage system referred to as the High Performance Antiproton Trap (HiPAT) with a goal of maintaining 10(exp 12) particles for up to 18 days. Experiments have been performed with this hardware using normal matter (positive hydrogen ions) to assess the device's ability to hold charged particles. These ions are currently created using an electron gun method to ionize background gas; however, this technique is limited by the quantity that can be captured. To circumvent this issue, an ion source is currently being commissioned which will greatly increase the number of ions captured and more closely simulate actual operations expected at an antiproton production facility. Ions have been produced, stored for various time intervals, and then extracted against detectors to measure species, quantity and energy. Radio frequency stabilization has been tested as a method to prolong ion lifetime: results show an increase in the baseline 1/e lifetime of trapped particles from hours to days. Impurities in the residual background gas (typically carbon-containing species CH4, CO, CO2, etc.) present a continuing problem by reducing the trapped hydrogen population through the mechanism of ion charge exchange.

  9. Network and user interface for PAT DOME virtual motion environment system

    NASA Technical Reports Server (NTRS)

    Worthington, J. W.; Duncan, K. M.; Crosier, W. G.

    1993-01-01

    The Device for Orientation and Motion Environments Preflight Adaptation Trainer (DOME PAT) provides astronauts a virtual microgravity sensory environment designed to help alleviate tye symptoms of space motion sickness (SMS). The system consists of four microcomputers networked to provide real time control, and an image generator (IG) driving a wide angle video display inside a dome structure. The spherical display demands distortion correction. The system is currently being modified with a new graphical user interface (GUI) and a new Silicon Graphics IG. This paper will concentrate on the new GUI and the networking scheme. The new GUI eliminates proprietary graphics hardware and software, and instead makes use of standard and low cost PC video (CGA) and off the shelf software (Microsoft's Quick C). Mouse selection for user input is supported. The new Silicon Graphics IG requires an Ethernet interface. The microcomputer known as the Real Time Controller (RTC), which has overall control of the system and is written in Ada, was modified to use the free public domain NCSA Telnet software for Ethernet communications with the Silicon Graphics IG. The RTC also maintains the original ARCNET communications through Novell Netware IPX with the rest of the system. The Telnet TCP/IP protocol was first used for real-time communication, but because of buffering problems the Telnet datagram (UDP) protocol needed to be implemented. Since the Telnet modules are written in C, the Adap pragma 'Interface' was used to interface with the network calls.

  10. Adoption of PERILIPIN as a unifying nomenclature for the mammalian PAT-family of intracellular lipid storage droplet proteins

    PubMed Central

    Kimmel, Alan R.; Brasaemle, Dawn L.; McAndrews-Hill, Monica; Sztalryd, Carole; Londos, Constantine

    2010-01-01

    The PAT family of proteins has been identified in eukaryotic species as diverse as vertebrates, insects, and amebazoa. These proteins share a highly conserved sequence organization and avidity for the surfaces of intracellular, neutral lipid storage droplets. The current nomenclature of the various members lacks consistency and precision, deriving more from historic context than from recognition of evolutionary relationship and shared function. In consultation with the Mouse Genomic Nomenclature Committee, the Human Genome Organization Genomic Nomenclature Committee, and conferees at the 2007 FASEB Conference on Lipid Droplets: Metabolic Consequences of the Storage of Neutral Lipids, we have established a unifying nomenclature for the gene and protein family members. Each gene member will incorporate the root term PERILIPIN (PLIN), the founding gene of the PAT family, with the different genes/proteins numbered sequentially. PMID:19638644

  11. Effects of EPA and DHA on lipid droplet accumulation and mRNA abundance of PAT proteins in caprine monocytes.

    PubMed

    Lecchi, Cristina; Invernizzi, Guido; Agazzi, Alessandro; Modina, Silvia; Sartorelli, Paola; Savoini, Giovanni; Ceciliani, Fabrizio

    2013-04-01

    The present study investigated the in vitro effects on caprine monocytes of two ω-3 PUFAs, namely eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) on lipid droplet formation, an emerging process of fundamental importance in innate immunity regulation. The mRNA abundance of PAT protein family (PLIN1, PLIN2 and PLIN3), involved in the formation and trafficking of the droplets, was also assessed. The effects of EPA and DHA on monocyte apoptosis were studied as well. The number of lipid droplets per cell was found to be dependent on both type and concentration of fatty acid. ω-3 PUFAs upregulated PLIN3 and PLIN2 gene expression, as well as apoptosis rate. The present findings suggest that PUFA might modify innate immune functions of goat monocytes by interfering with the formation of lipid droplets and by upregulating proteins belonging to PAT protein family.

  12. [The Patient Rights Act (PatRG)--Part 3: Burden of proof in liability questions and critical resumé].

    PubMed

    Parzeller, Markus; Zedler, Barbara

    2014-01-01

    The article deals with the new regulations in the German Civil Code (Bürgerliches Gesetzbuch - BGB), which came into effect in Germany as the Patient Rights Act (PatRG) on the 2/26/2013. In the third part, the burden of proof in liability questions (and 630h BGB) are discussed and critically analysed. In the discussion and a final resume points of criticism of the new law are pointed out.

  13. Comprehensive clinical studies in 34 patients with molecularly defined UPD(14)pat and related conditions (Kagami-Ogata syndrome).

    PubMed

    Kagami, Masayo; Kurosawa, Kenji; Miyazaki, Osamu; Ishino, Fumitoshi; Matsuoka, Kentaro; Ogata, Tsutomu

    2015-11-01

    Paternal uniparental disomy 14 (UPD(14)pat) and epimutations and microdeletions affecting the maternally derived 14q32.2 imprinted region lead to a unique constellation of clinical features such as facial abnormalities, small bell-shaped thorax with a coat-hanger appearance of the ribs, abdominal wall defects, placentomegaly, and polyhydramnios. In this study, we performed comprehensive clinical studies in patients with UPD(14)pat (n=23), epimutations (n=5), and microdeletions (n=6), and revealed several notable findings. First, a unique facial appearance with full cheeks and a protruding philtrum and distinctive chest roentgenograms with increased coat-hanger angles to the ribs constituted the pathognomonic features from infancy through childhood. Second, birth size was well preserved, with a median birth length of ±0 SD (range, -1.7 to +3.0 SD) and a median birth weight of +2.3 SD (range, +0.1 to +8.8 SD). Third, developmental delay and/or intellectual disability was invariably present, with a median developmental/intellectual quotient of 55 (range, 29-70). Fourth, hepatoblastoma was identified in three infantile patients (8.8%), and histological examination in two patients showed a poorly differentiated embryonal hepatoblastoma with focal macrotrabecular lesions and well-differentiated hepatoblastoma, respectively. These findings suggest the necessity of an adequate support for developmental delay and periodical screening for hepatoblastoma in the affected patients, and some phenotypic overlap between UPD(14)pat and related conditions and Beckwith-Wiedemann syndrome. On the basis of our previous and present studies that have made a significant contribution to the clarification of underlying (epi)genetic factors and the definition of clinical findings, we propose the name 'Kagami-Ogata syndrome' for UPD(14)pat and related conditions.

  14. Introducing uncertainty analysis of nucleation and crystal growth models in Process Analytical Technology (PAT) system design of crystallization processes.

    PubMed

    Samad, Noor Asma Fazli Abdul; Sin, Gürkan; Gernaey, Krist V; Gani, Rafiqul

    2013-11-01

    This paper presents the application of uncertainty and sensitivity analysis as part of a systematic model-based process monitoring and control (PAT) system design framework for crystallization processes. For the uncertainty analysis, the Monte Carlo procedure is used to propagate input uncertainty, while for sensitivity analysis, global methods including the standardized regression coefficients (SRC) and Morris screening are used to identify the most significant parameters. The potassium dihydrogen phosphate (KDP) crystallization process is used as a case study, both in open-loop and closed-loop operation. In the uncertainty analysis, the impact on the predicted output of uncertain parameters related to the nucleation and the crystal growth model has been investigated for both a one- and two-dimensional crystal size distribution (CSD). The open-loop results show that the input uncertainties lead to significant uncertainties on the CSD, with appearance of a secondary peak due to secondary nucleation for both cases. The sensitivity analysis indicated that the most important parameters affecting the CSDs are nucleation order and growth order constants. In the proposed PAT system design (closed-loop), the target CSD variability was successfully reduced compared to the open-loop case, also when considering uncertainty in nucleation and crystal growth model parameters. The latter forms a strong indication of the robustness of the proposed PAT system design in achieving the target CSD and encourages its transfer to full-scale implementation.

  15. The structure of the cyanobactin domain of unknown function from PatG in the patellamide gene cluster

    SciTech Connect

    Mann, Greg; Koehnke, Jesko; Bent, Andrew F.; Graham, Rachael; Houssen, Wael; Jaspars, Marcel; Schwarz-Linek, Uli; Naismith, James H.

    2014-11-14

    The highly conserved domain of unknown function in the cyanobactin superfamily has a novel fold. The protein does not appear to bind the most plausible substrates, leaving questions as to its role. Patellamides are members of the cyanobactin family of ribosomally synthesized and post-translationally modified cyclic peptide natural products, many of which, including some patellamides, are biologically active. A detailed mechanistic understanding of the biosynthetic pathway would enable the construction of a biotechnological ‘toolkit’ to make novel analogues of patellamides that are not found in nature. All but two of the protein domains involved in patellamide biosynthesis have been characterized. The two domains of unknown function (DUFs) are homologous to each other and are found at the C-termini of the multi-domain proteins PatA and PatG. The domain sequence is found in all cyanobactin-biosynthetic pathways characterized to date, implying a functional role in cyanobactin biosynthesis. Here, the crystal structure of the PatG DUF domain is reported and its binding interactions with plausible substrates are investigated.

  16. High-speed pre-clinical brain imaging using pulsed laser diode based photoacoustic tomography (PLD-PAT) system

    NASA Astrophysics Data System (ADS)

    Upputuri, Paul Kumar; Pramanik, Manojit

    2016-03-01

    Photoacoustic tomography (PAT) is a promising biomedical imaging modality for small animal imaging, breast cancer imaging, monitoring of vascularisation, tumor angiogenesis, blood oxygenation, total haemoglobin concentration etc. The existing PAT systems that uses Q-switched Nd:YAG and OPO nanosecond lasers have limitations in clinical applications because they are expensive, non-potable and not suitable for real-time imaging due to their low pulse repetition rate. Low-energy pulsed near-infrared diode laser which are low-cost, compact, and light-weight (<200 grams), can be used as an alternate. In this work, we present a photoacoustic tomography system with a pulsed laser diode (PLD) that can nanosecond pulses with pulse energy 1.3 mJ/pulse at ~803 nm wavelength and 7000 Hz repetition rate. The PLD is integrated inside a single-detector circular scanning geometric system. To verify the high speed imaging capabilities of the PLD-PAT system, we performed in vivo experimental results on small animal brain imaging using this system. The proposed system is portable, low-cost and can provide real-time imaging.

  17. Proton-assisted amino acid transporter PAT1 complexes with Rag GTPases and activates TORC1 on late endosomal and lysosomal membranes.

    PubMed

    Ögmundsdóttir, Margrét H; Heublein, Sabine; Kazi, Shubana; Reynolds, Bruno; Visvalingam, Shivanthy M; Shaw, Michael K; Goberdhan, Deborah C I

    2012-01-01

    Mammalian Target of Rapamycin Complex 1 (mTORC1) is activated by growth factor-regulated phosphoinositide 3-kinase (PI3K)/Akt/Rheb signalling and extracellular amino acids (AAs) to promote growth and proliferation. These AAs induce translocation of mTOR to late endosomes and lysosomes (LELs), subsequent activation via mechanisms involving the presence of intralumenal AAs, and interaction between mTORC1 and a multiprotein assembly containing Rag GTPases and the heterotrimeric Ragulator complex. However, the mechanisms by which AAs control these different aspects of mTORC1 activation are not well understood. We have recently shown that intracellular Proton-assisted Amino acid Transporter 1 (PAT1)/SLC36A1 is an essential mediator of AA-dependent mTORC1 activation. Here we demonstrate in Human Embryonic Kidney (HEK-293) cells that PAT1 is primarily located on LELs, physically interacts with the Rag GTPases and is required for normal AA-dependent mTOR relocalisation. We also use the powerful in vivo genetic methodologies available in Drosophila to investigate the regulation of the PAT1/Rag/Ragulator complex. We show that GFP-tagged PATs reside at both the cell surface and LELs in vivo, mirroring PAT1 distribution in several normal mammalian cell types. Elevated PI3K/Akt/Rheb signalling increases intracellular levels of PATs and synergistically enhances PAT-induced growth via a mechanism requiring endocytosis. In light of the recent identification of the vacuolar H(+)-ATPase as another Rag-interacting component, we propose a model in which PATs function as part of an AA-sensing engine that drives mTORC1 activation from LEL compartments.

  18. The effect of glycerol on the activity of beta-glucosidase from Botryodiplodia theobromae Pat.

    PubMed Central

    Umezurike, G M

    1988-01-01

    1. In the activity of the high-Mr beta-glucosidase A (beta-D-glucoside glucohydrolase, EC 3.2.1.21) obtained from culture filtrates of Botryodiplodia theobromae Pat. on o-nitrophenyl beta-D-glucopyranoside as substrate, both Vmax. and Km increased non-linearly with increasing concentration of glycerol, and the Vmax./Km(app.) ratio decreased non-linearly with increasing concentration of glycerol. 2. No increase in rate was observed with phenyl beta-D-glucopyranoside as substrate in the presence of up to 250 mM-glycerol, indicating that glucosylation is rate-limiting with this substrate. 3. With o-nitrophenyl beta-D-glucopyranoside, p-nitrophenyl beta-D-glucopyranoside and phenyl beta-D-glucopyranoside as substrates, kappa cat. values of 793.7 s-1, 62.8 s-1 and 5.4 s-1 respectively were calculated. 4. With o-nitrophenyl beta-D-glucopyranoside and phenyl beta-D-glucopyranoside as substrate, alpha-deuterium kinetic isotope effects of 1.9 +/- 0.03 and 1.01 +/- 0.01 respectively were found; in the presence of 200 mM-glycerol the values were 1.21 +/- 0.03 and 1.02 +/- 0.01 respectively. 5. In the presence of a large excess of o-nitrophenyl beta-D-glucopyranoside [( S] = 35.7 Km), the amount of o-nitrophenol and also of the transglucosylation product formed by beta-glucosidase action increased non-linearly, whereas that of glucose formed decreased non-linearly with increasing glycerol concentration. 6. All these results were found to fit the data calculated from rate equations derived on the basis of the proposed mechanism of enzyme action involving two ion-pair intermediates and a covalent alpha-D-glucosyl-enzyme in the reaction sequence [Umezurike (1987) Biochem. J. 241, 455-462]. PMID:3140799

  19. The Formation and Chronology of the PAT 91501 Impact-Melt L-Chondrite with Vesicle-Metal-Sulfide Assemblages

    NASA Technical Reports Server (NTRS)

    Benedix, G. K.; Ketcham, R. A.; Wilson, L.; McCoy, T. J.; Bogard, D. D.; Garrison, D. H.; Herzog, G. F.; Xue, S.; Klein, J.; Middleton, R.

    2007-01-01

    The L chondrite Patuxent Range (PAT) 41 91501 is an 8.5-kg unshocked, homogeneous, igneous-textured impact melt that cooled slowly compared to other meteoritic impact melts in a crater floor melt sheet or sub-crater dike. We conducted mineralogical and tomographic studies of previously unstudied mm- to cm-sized metal-sulfide-vesicle assemblages and chronologic studies of the silicate host. Metal-sulfide clasts constitute about 1 vol.%, comprise zoned taenite, troilite and pentlandite, and exhibit a consistent orientation between metal and sulfide and of metal-sulfide contacts. Vesicles make up approximately 2 vol.% and exhibit a similar orientation of long axes. Ar-39-Ar-40 measurements date the time of impact at 4.461 +/- 0.008 Gyr B.P. Cosmogenic noble gases and Be-10 and Al-2l activities suggest a pre-atmospheric radius of 40-60 cm and a cosmic ray exposure age of 25-29 Myr, similar to ages of a cluster of L chondrites. PAT 91501 dates the oldest known impact on the L chondrite parent body. The dominant vesicle-forming gas was S2 (approximately 15-20 ppm), which formed in equilibrium with impact-melted sulfides. The meteorite formed in an impact melt dike beneath a crater, as did other impact melted L chondrites, such as Chico. Cooling and solidification occurred over approximately 2 hours. During this time, approximately 90% of metal and sulfide segregated from the local melt. Remaining metal and sulfide grains oriented themselves in the local gravitational field, a feature nearly unique among meteorites. Many of these metal sulfide grains adhered to vesicles to form aggregates that may have been close to neutrally buoyant. These aggregates would have been carried upward with the residual melt, inhibiting further buoyancy-driven segregation. Although similar processes operated individually in other chondritic impact melts, their interaction produced the unique assemblage observed in PAT 91501.

  20. Kinderhookian (Lower Mississippian) calcareous rocks of the Howard Pass quadrangle, western Brooks Range: A section in Geologic studies in Alaska by the U.S. Geological Survey, 1995

    USGS Publications Warehouse

    Dumoulin, Julie A.; Harris, Anita G.

    1997-01-01

    Calcareous rocks of Kinderhookian (early Early Mississippian) age are widely distributed across the Howard Pass quadrangle in the western Brooks Range. Most occur in the lower part of the Lisburne Group (herein called the Rough Mountain Creek unit) and the upper part of the Endicott Group (Kayak Shale) in two sequences (Key Creek and Aniuk River) of the Endicott Mountains allochthon. Kinderhookian strata are also found in the Kelly River allochthon (Utukok Formation?) and in sections of uncertain stratigraphic affinity and structural level spatially associated with mafic volcanic rocks.Predominant Kinderhookian lithologies in the Lisburne Group are skeletal supportstone (rich in pelmatozoans, bryozoans, and brachiopods) and lesser spiculite; skeletal supportstone and calcarenite are the chief calcareous rock types in the Kayak Shale. Conodont and brachiopod faunas indicate that all of the Rough Mountain Creek unit and much of the Kayak Shale in the study area are of late Kinderhookian age. Lithologic and paleontologic data suggest that Kinderhookian strata in the Howard Pass quadrangle were deposited largely in inner- and middle-shelf settings with normal marine salinity and locally high energy. Overall, calcareous beds in the Rough Mountain Creek unit accumulated in a wider range of environments, less subject to siliciclastic input, than did calcareous beds in the Kayak, and Kinderhookian beds of both units in the Key Creek sequence formed in less diverse, somewhat shallower environments than correlative rocks in the Aniuk River sequence. Lithofacies patterns and contact relations imply that decreased siliciclastic influx, perhaps accompanied by relative sea-level rise, initiated deposition of the Rough Mountain Creek unit; relative sea-level rise and concurrent circulatory restriction most likely ended its deposition.Kinderhookian calcareous rocks in the Howard Pass quadrangle have several implications for middle Paleozoic paleogeography of the western Brooks

  1. Helicopter AFMAG (ZTEM) EM and magnetic results over sedimentary exhalative (SEDEX) lead-zinc deposits at Howard's Pass in Selwyn Basin, Yukon

    NASA Astrophysics Data System (ADS)

    Legault, Jean M.; Latrous, Ali; Zhao, Shengkai; Bournas, Nasreddine; Plastow, Geoffrey C.; Xue, Gabriel Guang

    2016-01-01

    In 2008, a regional scale 24,675 line-km survey covering a 25,000 km2 area (1 km line spacing) was flown in the Selwyn Basin. The survey footprint straddles east-central Yukon and overlaps into the western North-west Territories. In March 2013, Yukon Geological Survey purchased the survey data and, in November 2013, released the data publicly. The Selwyn Basin area is prospective for sedimentary exhalative (SEDEX)-style Pb-Zn-Ag mineralisation and the z-axis tipper electromagnetic (ZTEM) survey data provide insights into regional structures and plutons in the region. The survey overflew the Howard's Pass SEDEX deposits at the south-eastern edge of the Selwyn Basin survey area that hosts a ~250 million tonne resource with ~4.5% Zn and ~1.5% Pb. Airborne geophysics has not been extensively used in SEDEX exploration of the Selwyn Basin and the ZTEM survey is one of the few publicly available airborne audio-frequency magnetic (AFMAG) EM-magnetic datasets that offer the opportunity to study the deposit response at Howard's Pass in close detail. Rock physical properties indicate that the lowest resistivities are associated with the Road River Group that contains the Pb-Zn mineralised horizon at Howard's Pass, but also include graphitic shales in the same formation. Major NW-SE to ESE and minor NNW-SSE linear conductive trends correlate with known regional geologic, structural and inferred mineral trends that were previously not visible in magnetic results. At the deposit scale, a thin NW-SE trending conductive lineament extends along the > 37-km-long `Zinc Corridor' horizon at Howard's Pass, but must include both the Pb-Zn sulphide mineralisation deposit horizon as well as the surrounding graphitic black shales. 2D and 3D ZTEM inversions reveal zones of enhanced conductivity along strike and at depth that appear to correlate with the clustering of Pb-Zn deposits, which had not been previously noticed.

  2. Some implications for the study of the doctor-patient interaction: power, structure, and agency in the works of Howard Waitzkin and Arthur Kleinman.

    PubMed

    Pappas, G

    1990-01-01

    This article critiques two major theoretical perspectives concerning the doctor-patient interaction in medical anthropology represented by the work of Howard Waitzkin and Arthur Kleinman. In his work on the doctor-patient interaction Waitzkin has tended to draw on structural explanations which subordinate the role of agency. Kleinman's work emphasizes agency without satisfactorily integrating structural or social causality in his work on the doctor-patient interaction. The work of Anthony Giddens and others has clarified the structure/agency dichotomy in social science to which the nature of power is central.

  3. Ways of knowing: Howard Stein's border-crossing use of poetry to interrogate clinical medicine, medical education, and health care organizations.

    PubMed

    Shapiro, Johanna

    2016-09-01

    This article explores how medical anthropologist Howard Stein's poetry and his unique practice of sharing this poetry with the patients, physicians, and administrators who inspired it create ways of knowing that are at once revelatory and emancipatory. Stein's writing shows readers that poetry can be considered as a form of data and as a method of investigation into the processes of the human soul. Furthermore, it represents a kind of intervention that invites health professional readers toward connection, bridge building, and solidarity with their patients and with one another. (PsycINFO Database Record

  4. Howard University Symposium on Nonlinear Semigroups, Partial Differential Equations and Attractors (2nd) Held in Washington, D. C. on 3-7 August 1987.

    DTIC Science & Technology

    1987-09-30

    Building GENERAL INTEREST Chairman: Luis Vazquez, Universidad Complutense , Madrid 8:30 - 9:15 a.m. Robert Sternberg, ONR, Boston "Symmetry in Geometrical...Universite de Paris VI "Some Remarks on Nonlinear Schrodinger Equations" 10:45 - 11:15 a.m. Coffee Break 11:15 - 12:00 noon Luis Vazquez, Universidad ... Complutense , MTadrid "The Finite Difference Method in the Quantum Theory" 12:00 - 12:45 p.m. Walter Miller, Howard University "Dynamics of Periodically

  5. Review of the High Performance Antiproton Trap (HiPAT) Experiment at the Marshall Space Flight Center

    NASA Technical Reports Server (NTRS)

    Pearson, J. B.; Sims, Herb; Martin, James; Chakrabarti, Suman; Lewis, Raymond; Fant, Wallace

    2003-01-01

    The significant energy density of matter-antimatter annihilation is attractive to the designers of future space propulsion systems, with the potential to offer a highly compact source of power. Many propulsion concepts exist that could take advantage of matter-antimatter reactions, and current antiproton production rates are sufficient to support basic proof-of-principle evaluation of technology associated with antimatter- derived propulsion. One enabling technology for such experiments is portable storage of low energy antiprotons, allowing antiprotons to be trapped, stored, and transported for use at an experimental facility. To address this need, the Marshall Space Flight Center's Propulsion Research Center is developing a storage system referred to as the High Performance Antiproton Trap (HiPAT) with a design goal of containing 10(exp 12) particles for up to 18 days. The HiPAT makes use of an electromagnetic system (Penning- Malmberg design) consisting of a 4 Telsa superconductor, high voltage electrode structure, radio frequency (RF) network, and ultra high vacuum system. To evaluate the system normal matter sources (both electron guns and ion sources) are used to generate charged particles. The electron beams ionize gas within the trapping region producing ions in situ, whereas the ion sources produce the particles external to the trapping region and required dynamic capture. A wide range of experiments has been performed examining factors such as ion storage lifetimes, effect of RF energy on storage lifetime, and ability to routinely perform dynamic ion capture. Current efforts have been focused on improving the FW rotating wall system to permit longer storage times and non-destructive diagnostics of stored ions. Typical particle detection is performed by extracting trapped ions from HiPAT and destructively colliding them with a micro-channel plate detector (providing number and energy information). This improved RF system has been used to detect various

  6. A Scintillation Counter System Design To Detect Antiproton Annihilation using the High Performance Antiproton Trap(HiPAT)

    NASA Technical Reports Server (NTRS)

    Martin, James J.; Lewis, Raymond A.; Stanojev, Boris

    2003-01-01

    The High Performance Antiproton Trap (HiPAT), a system designed to hold up to l0(exp 12) charge particles with a storage half-life of approximately 18 days, is a tool to support basic antimatter research. NASA's interest stems from the energy density represented by the annihilation of matter with antimatter, 10(exp 2)MJ/g. The HiPAT is configured with a Penning-Malmberg style electromagnetic confinement region with field strengths up to 4 Tesla, and 20kV. To date a series of normal matter experiments, using positive and negative ions, have been performed evaluating the designs performance prior to operations with antiprotons. The primary methods of detecting and monitoring stored normal matter ions and antiprotons within the trap includes a destructive extraction technique that makes use of a micro channel plate (MCP) device and a non-destractive radio frequency scheme tuned to key particle frequencies. However, an independent means of detecting stored antiprotons is possible by making use of the actual annihilation products as a unique indicator. The immediate yield of the annihilation event includes photons and pie mesons, emanating spherically from the point of annihilation. To "count" these events, a hardware system of scintillators, discriminators, coincident meters and multi channel scalars (MCS) have been configured to surround much of the HiPAT. Signal coincidence with voting logic is an essential part of this system, necessary to weed out the single cosmic ray events from the multi-particle annihilation shower. This system can be operated in a variety of modes accommodating various conditions. The first is a low-speed sampling interval that monitors the background loss or "evaporation" rate of antiprotons held in the trap during long storage periods; provides an independent method of validating particle lifetimes. The second is a high-speed sample rate accumulating information on a microseconds time-scale; useful when trapped antiparticles are extracted

  7. Discharge diversion in the Patía River delta, the Colombian Pacific: Geomorphic and ecological consequences for mangrove ecosystems

    NASA Astrophysics Data System (ADS)

    Restrepo, Juan D.; Cantera, Jaime R.

    2013-10-01

    In the Patía River delta, the best-developed delta on the western margin of South America, a major water diversion started in 1972. The diversion of the Patía flow to the Sanquianga River, the latter a small stream draining internal lakes from the Pacific lowlands, shifted the active delta plain from the south to the north and changed the northern estuarine system into an active delta plain. The Sanquianga Mangrove National Park, a mangrove reserve measuring 800 km2, lies in this former estuary, where major hydrologic and sedimentation changes are occurring. Overall, major environmental consequences of this discharge diversion in terms of geomorphic changes along distributary channels and ecological impacts on mangrove ecosystems are evidenced by: (1) distributary channel accretion by operating processes such as sedimentation, overbank flow, increasing width of levees, sedimentation in crevasses, interdistributary channel fill, and colonization of pioneer mangrove; (2) freshening conditions in the Sanquianga distributary channel, a hydrologic change that has shifted the upper estuarine region (salinity <1%) downstream; (3) downstream advance of freshwater vegetation, which is invading channel banks in the lower and mixing estuarine zones; (4) die-off of approximately 5200 ha of mangrove near the delta apex at Bocas de Satinga, where the highest sediment accumulation rates occur; and (5) recurrent periods of mangrove defoliation due to a worm plague. Further analyses indicate strong mangrove erosion along transgressive barrier islands on the former delta plain. Here tectonic-induced subsidence, relative sea-level rise, and sediment starving conditions due to the channel diversion, are the main causes of the observed retreating conditions of mangrove communities. Our data also indicate that the Patía River has the highest sediment load (27 × 106 t yr-1) and basin-wide sediment yield (1500 t km-2 yr-1) on the west coast of South America. Erosion rates from the Pat

  8. PAT4 levels control amino-acid sensitivity of rapamycin-resistant mTORC1 from the Golgi and affect clinical outcome in colorectal cancer.

    PubMed

    Fan, S-J; Snell, C; Turley, H; Li, J-L; McCormick, R; Perera, S M W; Heublein, S; Kazi, S; Azad, A; Wilson, C; Harris, A L; Goberdhan, D C I

    2016-06-09

    Tumour cells can use strategies that make them resistant to nutrient deprivation to outcompete their neighbours. A key integrator of the cell's responses to starvation and other stresses is amino-acid-dependent mechanistic target of rapamycin complex 1 (mTORC1). Activation of mTORC1 on late endosomes and lysosomes is facilitated by amino-acid transporters within the solute-linked carrier 36 (SLC36) and SLC38 families. Here, we analyse the functions of SLC36 family member, SLC36A4, otherwise known as proton-assisted amino-acid transporter 4 (PAT4), in colorectal cancer. We show that independent of other major pathological factors, high PAT4 expression is associated with reduced relapse-free survival after colorectal cancer surgery. Consistent with this, PAT4 promotes HCT116 human colorectal cancer cell proliferation in culture and tumour growth in xenograft models. Inducible knockdown in HCT116 cells reveals that PAT4 regulates a form of mTORC1 with two distinct properties: first, it preferentially targets eukaryotic translation initiation factor 4E-binding protein 1 (4E-BP1), and second, it is resistant to rapamycin treatment. Furthermore, in HCT116 cells two non-essential amino acids, glutamine and serine, which are often rapidly metabolised by tumour cells, regulate rapamycin-resistant mTORC1 in a PAT4-dependent manner. Overexpressed PAT4 is also able to promote rapamycin resistance in human embryonic kidney-293 cells. PAT4 is predominantly associated with the Golgi apparatus in a range of cell types, and in situ proximity ligation analysis shows that PAT4 interacts with both mTORC1 and its regulator Rab1A on the Golgi. These findings, together with other studies, suggest that differentially localised intracellular amino-acid transporters contribute to the activation of alternate forms of mTORC1. Furthermore, our data predict that colorectal cancer cells with high PAT4 expression will be more resistant to depletion of serine and glutamine, allowing them to

  9. PAT4 levels control amino-acid sensitivity of rapamycin-resistant mTORC1 from the Golgi and affect clinical outcome in colorectal cancer

    PubMed Central

    Fan, S-J; Snell, C; Turley, H; Li, J-L; McCormick, R; Perera, S M W; Heublein, S; Kazi, S; Azad, A; Wilson, C; Harris, A L; Goberdhan, D C I

    2016-01-01

    Tumour cells can use strategies that make them resistant to nutrient deprivation to outcompete their neighbours. A key integrator of the cell's responses to starvation and other stresses is amino-acid-dependent mechanistic target of rapamycin complex 1 (mTORC1). Activation of mTORC1 on late endosomes and lysosomes is facilitated by amino-acid transporters within the solute-linked carrier 36 (SLC36) and SLC38 families. Here, we analyse the functions of SLC36 family member, SLC36A4, otherwise known as proton-assisted amino-acid transporter 4 (PAT4), in colorectal cancer. We show that independent of other major pathological factors, high PAT4 expression is associated with reduced relapse-free survival after colorectal cancer surgery. Consistent with this, PAT4 promotes HCT116 human colorectal cancer cell proliferation in culture and tumour growth in xenograft models. Inducible knockdown in HCT116 cells reveals that PAT4 regulates a form of mTORC1 with two distinct properties: first, it preferentially targets eukaryotic translation initiation factor 4E-binding protein 1 (4E-BP1), and second, it is resistant to rapamycin treatment. Furthermore, in HCT116 cells two non-essential amino acids, glutamine and serine, which are often rapidly metabolised by tumour cells, regulate rapamycin-resistant mTORC1 in a PAT4-dependent manner. Overexpressed PAT4 is also able to promote rapamycin resistance in human embryonic kidney-293 cells. PAT4 is predominantly associated with the Golgi apparatus in a range of cell types, and in situ proximity ligation analysis shows that PAT4 interacts with both mTORC1 and its regulator Rab1A on the Golgi. These findings, together with other studies, suggest that differentially localised intracellular amino-acid transporters contribute to the activation of alternate forms of mTORC1. Furthermore, our data predict that colorectal cancer cells with high PAT4 expression will be more resistant to depletion of serine and glutamine, allowing them to

  10. Cloning and characterization of a new gene from the PAT protein family, in a marsupial, the stripe-faced dunnart (Sminthopsis macroura).

    PubMed

    Au, Phil Chi Khang; Selwood, Lynne; Familari, Mary

    2010-04-01

    Recent studies of PAT proteins in Drosophila and Xenopus have revealed significant roles for this family of proteins in the polarized transport of lipid droplets and maternal determinants during early embryogenesis. In mammals, PAT proteins are known to function mainly in lipid metabolism, yet research has yet to establish a role for PAT proteins in mammalian embryogenesis. Oocytes and early cleavage stages in Sminthopsis macroura show obvious polarized cytoplasmic distribution of organelles, somewhat similar to Drosophila and Xenopus, suggesting that a PAT protein may also be involved in S. macroura embryonic development. In the present study, we identified a new marsupial gene for PAT family proteins, DPAT, from S. macroura. Expression analyses by RT-PCR and whole mount fluorescent in situ hybridization revealed that DPAT expression was specific to oocytes and cleavage stage conceptuses. Analysis of the localization of lipid droplets during S. macroura early embryonic development found a polarized distribution of lipid droplets at the two- and four-cell stage, and an asymmetric enrichment in blastomeres on one side of conceptuses from two- to eight-cell stage. Lipid droplets largely segregate to pluriblast cells at the 16-cell stage, suggesting a role in pluriblast lineage allocation.

  11. Structural basis for selective recognition of acyl chains by the membrane-associated acyltransferase PatA

    PubMed Central

    Albesa-Jové, David; Svetlíková, Zuzana; Tersa, Montse; Sancho-Vaello, Enea; Carreras-González, Ana; Bonnet, Pascal; Arrasate, Pedro; Eguskiza, Ander; Angala, Shiva K.; Cifuente, Javier O.; Korduláková, Jana; Jackson, Mary; Mikušová, Katarína; Guerin, Marcelo E.

    2016-01-01

    The biosynthesis of phospholipids and glycolipids are critical pathways for virtually all cell membranes. PatA is an essential membrane associated acyltransferase involved in the biosynthesis of mycobacterial phosphatidyl-myo-inositol mannosides (PIMs). The enzyme transfers a palmitoyl moiety from palmitoyl–CoA to the 6-position of the mannose ring linked to 2-position of inositol in PIM1/PIM2. We report here the crystal structures of PatA from Mycobacterium smegmatis in the presence of its naturally occurring acyl donor palmitate and a nonhydrolyzable palmitoyl–CoA analog. The structures reveal an α/β architecture, with the acyl chain deeply buried into a hydrophobic pocket that runs perpendicular to a long groove where the active site is located. Enzyme catalysis is mediated by an unprecedented charge relay system, which markedly diverges from the canonical HX4D motif. Our studies establish the mechanistic basis of substrate/membrane recognition and catalysis for an important family of acyltransferases, providing exciting possibilities for inhibitor design. PMID:26965057

  12. Small GTPase CDC-42 promotes apoptotic cell corpse clearance in response to PAT-2 and CED-1 in C. elegans.

    PubMed

    Neukomm, L J; Zeng, S; Frei, A P; Huegli, P A; Hengartner, M O

    2014-06-01

    The rapid clearance of dying cells is important for the well-being of multicellular organisms. In C. elegans, cell corpse removal is mainly mediated by three parallel engulfment signaling cascades. These pathways include two small GTPases, MIG-2/RhoG and CED-10/Rac1. Here we present the identification and characterization of CDC-42 as a third GTPase involved in the regulation of cell corpse clearance. Genetic analyses performed by both loss of cdc-42 function and cdc-42 overexpression place cdc-42 in parallel to the ced-2/5/12 signaling module, in parallel to or upstream of the ced-10 module, and downstream of the ced-1/6/7 module. CDC-42 accumulates in engulfing cells at membranes surrounding apoptotic corpses. The formation of such halos depends on the integrins PAT-2/PAT-3, UNC-112 and the GEF protein UIG-1, but not on the canonical ced-1/6/7 or ced-2/5/12 signaling modules. Together, our results suggest that the small GTPase CDC-42 regulates apoptotic cell engulfment possibly upstream of the canonical Rac GTPase CED-10, by polarizing the engulfing cell toward the apoptotic corpse in response to integrin signaling and ced-1/6/7 signaling in C. elegans.

  13. Strategic funding priorities in the pharmaceutical sciences allied to Quality by Design (QbD) and Process Analytical Technology (PAT).

    PubMed

    Aksu, Buket; De Beer, Thomas; Folestad, Staffan; Ketolainen, Jarkko; Lindén, Hans; Lopes, Joao Almeida; de Matas, Marcel; Oostra, Wim; Rantanen, Jukka; Weimer, Marco

    2012-09-29

    Substantial changes in Pharmaceutical R&D strategy are required to address existing issues of low productivity, imminent patent expirations and pressures on pricing. Moves towards personalized healthcare and increasing diversity in the nature of portfolios including the rise of biopharmaceuticals however have the potential to provide considerable challenges to the establishment of cost effective and robust supply chains. To guarantee product quality and surety of supply for essential medicines it is necessary that manufacturing science keeps pace with advances in pharmaceutical R&D. In this position paper, the EUFEPS QbD and PAT Sciences network make recommendations that European industry, academia and health agencies focus attention on delivering step changes in science and technology in a number of key themes. These subject areas, all underpinned by the sciences allied to QbD and PAT, include product design and development for personalized healthcare, continuous-processing in pharmaceutical product manufacture, quantitative quality risk assessment for pharmaceutical development including life cycle management and the downstream processing of biopharmaceutical products. Plans are being established to gain commitment for inclusion of these themes into future funding priorities for the Innovative Medicines Initiative (IMI).

  14. Structural basis for selective recognition of acyl chains by the membrane-associated acyltransferase PatA.

    PubMed

    Albesa-Jové, David; Svetlíková, Zuzana; Tersa, Montse; Sancho-Vaello, Enea; Carreras-González, Ana; Bonnet, Pascal; Arrasate, Pedro; Eguskiza, Ander; Angala, Shiva K; Cifuente, Javier O; Korduláková, Jana; Jackson, Mary; Mikušová, Katarína; Guerin, Marcelo E

    2016-03-11

    The biosynthesis of phospholipids and glycolipids are critical pathways for virtually all cell membranes. PatA is an essential membrane associated acyltransferase involved in the biosynthesis of mycobacterial phosphatidyl-myo-inositol mannosides (PIMs). The enzyme transfers a palmitoyl moiety from palmitoyl-CoA to the 6-position of the mannose ring linked to 2-position of inositol in PIM1/PIM2. We report here the crystal structures of PatA from Mycobacterium smegmatis in the presence of its naturally occurring acyl donor palmitate and a nonhydrolyzable palmitoyl-CoA analog. The structures reveal an α/β architecture, with the acyl chain deeply buried into a hydrophobic pocket that runs perpendicular to a long groove where the active site is located. Enzyme catalysis is mediated by an unprecedented charge relay system, which markedly diverges from the canonical HX4D motif. Our studies establish the mechanistic basis of substrate/membrane recognition and catalysis for an important family of acyltransferases, providing exciting possibilities for inhibitor design.

  15. Crocin and quercetin prevent PAT-induced apoptosis in mammalian cells: Involvement of ROS-mediated ER stress pathway.

    PubMed

    Boussabbeh, Manel; Prola, Alexandre; Ben Salem, Intidhar; Guilbert, Arnaud; Bacha, Hassen; Lemaire, Christophe; Abis-Essefi, Salwa

    2015-08-27

    Patulin (PAT) is a secondary metabolite produced by several species of the genera of Penicillium, Aspergillus, and Byssochlamys that can be found in rotting fruits, especially in apples and apple-based products. Exposure to this mycotoxin has been reported to induce intestinal and kidney injuries. The mechanism underlying such toxicity has been linked to the induction of apoptosis which occurred with reactive oxygen species production and endoplasmic reticulum (ER) stress induction. This study aimed to evaluate the effect of the two common dietary compounds Quercetin (QUER), a natural flavonoid, and Crocin (CRO), a natural carotenoid, on PAT-induced toxicity in human colon carcinoma (HCT116) and embryonic kidney cells (HEK293). We showed that antioxidant properties of QUER and CRO help to prevent ER stress activation and lipid peroxidation as evidenced by the reduction in GRP78 and GADD34 expressions and the decrease in malondialdehyde production. Furthermore, we demonstrated their ability to re-establish the loss of the mitochondrial membrane potential to inhibit caspase 3 activation and DNA fragmentation. © 2015 Wiley Periodicals, Inc. Environ Toxicol, 2015.

  16. Human induced discharge diversion in a tropical delta and its environmental implications: The Patía River, Colombia

    NASA Astrophysics Data System (ADS)

    Restrepo, Juan D.; Kettner, Albert

    2012-03-01

    SummaryThe Patía River, the number one in terms of sediment yield ˜1500 t km-2 yr-1 draining the western South America, has the most extensive and well developed delta on the Pacific coast, measuring 1700 km2. During the Holocene, nature forced the Patía delta to the south; however, a major water diversion, starting in 1972, diverted the Patía flow to the Sanguianga River, the latter, a small stream draining internal lakes from the Pacific lowlands. This human induced discharge diversion shifted the active delta plain back to the north and changed the northern estuarine system into an active delta plain. Overall, major environmental consequences of this discharge diversion in terms of morphological changes along the delta coast and distributary channels, are evidenced by: (1) coastal retreat along the abandoned delta lobe; 63% of the southern shoreline is retreating at maximum rates of 7 m yr-1, with a corresponding coastal land loss of 106 m yr-1; (2) transgressive barrier islands with exposed peat soils in the surf zone; (3) abandonment of former active distributaries in the southern delta plain with associated closing of inlets and formation of ebb tidal deltas; (4) breaching events on barrier islands; and (5) distributary channel accretion in the northern delta plain by morphological processes such as sedimentation (also in crevasses), overbank flow, increasing width of levees, interdistributary channel fill, and colonization of pioneer mangrove. The Sanguianga Mangrove National Park (SMNP), the largest mangrove reserve in Colombia, measuring 800 km2, lies in this former estuary, where major hydrologic and sedimentation changes are occurring. Observed environmental changes in the SMNP, include (1) seaward advance of the sub-aqueous delta front at the Sanquianga inlet evidenced by an increase in tidal flat area from 5.4 Mm2 in 1986 to 14 Mm2 in 2001; (2) freshening conditions in the Sanguianga distributary channel, a hydrologic change that has shifted the

  17. The mini-PAT as a multi-source feedback tool for trainees in child and adolescent psychiatry: assessing whether it is fit for purpose

    PubMed Central

    Salmon, Gill; Pugsley, Lesley

    2017-01-01

    This paper discusses the research supporting the use of multi-source feedback (MSF) for doctors and describes the mini-Peer Assessment Tool (mini-PAT), the MSF instrument currently used to assess trainees in child and adolescent psychiatry. The relevance of issues raised in the literature about MSF tools in general is examined in relation to trainees in child and adolescent psychiatry as well as the appropriateness of the mini-PAT for this group. Suggestions for change including modifications to existing MSF tools or the development of a specialty-specific MSF instrument are offered.

  18. Hybrid modeling for quality by design and PAT-benefits and challenges of applications in biopharmaceutical industry.

    PubMed

    von Stosch, Moritz; Davy, Steven; Francois, Kjell; Galvanauskas, Vytautas; Hamelink, Jan-Martijn; Luebbert, Andreas; Mayer, Martin; Oliveira, Rui; O'Kennedy, Ronan; Rice, Paul; Glassey, Jarka

    2014-06-01

    This report highlights the drivers, challenges, and enablers of the hybrid modeling applications in biopharmaceutical industry. It is a summary of an expert panel discussion of European academics and industrialists with relevant scientific and engineering backgrounds. Hybrid modeling is viewed in its broader sense, namely as the integration of different knowledge sources in form of parametric and nonparametric models into a hybrid semi-parametric model, for instance the integration of fundamental and data-driven models. A brief description of the current state-of-the-art and industrial uptake of the methodology is provided. The report concludes with a number of recommendations to facilitate further developments and a wider industrial application of this modeling approach. These recommendations are limited to further exploiting the benefits of this methodology within process analytical technology (PAT) applications in biopharmaceutical industry.

  19. Decay of Fecal Indicator Bacterial Populations and Bovine-Associated Source-Tracking Markers in Freshly Deposited Cow Pats

    PubMed Central

    Oladeinde, Adelumola; Bohrmann, Thomas; Wong, Kelvin; Purucker, S. T.; Bradshaw, Ken; Brown, Reid; Snyder, Blake

    2014-01-01

    Understanding the survival of fecal indicator bacteria (FIB) and microbial source-tracking (MST) markers is critical to developing pathogen fate and transport models. Although pathogen survival in water microcosms and manure-amended soils is well documented, little is known about their survival in intact cow pats deposited on pastures. We conducted a study to determine decay rates of fecal indicator bacteria (Escherichia coli and enterococci) and bovine-associated MST markers (CowM3, Rum-2-bac, and GenBac) in 18 freshly deposited cattle feces from three farms in northern Georgia. Samples were randomly assigned to shaded or unshaded treatment in order to determine the effects of sunlight, moisture, and temperature on decay rates. A general linear model (GLM) framework was used to determine decay rates. Shading significantly decreased the decay rate of the E. coli population (P < 0.0001), with a rate of −0.176 day−1 for the shaded treatment and −0.297 day−1 for the unshaded treatment. Shading had no significant effect on decay rates of enterococci, CowM3, Rum-2-bac, and GenBac (P > 0.05). In addition, E. coli populations showed a significant growth rate (0.881 day−1) in the unshaded samples during the first 5 days after deposition. UV-B was the most important parameter explaining the decay rate of E. coli populations. A comparison of the decay behaviors among all markers indicated that enterococcus concentrations exhibit a better correlation with the MST markers than E. coli concentrations. Our results indicate that bovine-associated MST markers can survive in cow pats for at least 1 month after excretion, and although their decay dynamic differs from the decay dynamic of E. coli populations, they seem to be reliable markers to use in combination with enterococci to monitor fecal pollution from pasture lands. PMID:24141130

  20. Strategic framework for education and training in Quality by Design (QbD) and process analytical technology (PAT).

    PubMed

    de Matas, Marcel; De Beer, Thomas; Folestad, Staffan; Ketolainen, Jarkko; Lindén, Hans; Lopes, João Almeida; Oostra, Wim; Weimer, Marco; Öhrngren, Per; Rantanen, Jukka

    2016-07-30

    The regulatory and technical landscape of the pharmaceutical field is rapidly evolving from one focused predominantly on development of small molecules, using well established manufacturing technologies towards an environment in which biologicals and complex modalities are being developed using advanced science and technology coupled with the application of modern Quality by Design (QbD) principles. In order that Europe keeps pace with these changes and sustains its position as major player in the development and commercialization of medicines, it is essential that measures are put in place to maintain a highly skilled workforce. A number of challenges however exist to equipping academic, industrial and health agency staff with the requisite knowledge, skills and experience to develop the next generation of medicines. In this regard, the EUFEPS QbD and PAT Sciences Network has proposed a structured framework for education, training and continued professional development, which comprises a number of pillars covering the fundamental principles of modern pharmaceutical development including the underpinning aspects of science, engineering and technology innovation. The framework is not prescriptive and is not aimed at describing specific course content in detail. It should however be used as a point of reference for those institutions delivering pharmaceutical based educational courses, to ensure that the necessary skills, knowledge and experience for successful pharmaceutical development are maintained. A positive start has been made and a number of examples of formal higher education courses and short training programs containing elements of this framework have been described. The ultimate vision for this framework however, is to see widespread adoption and proliferation of this curriculum with it forming the backbone of QbD and PAT science based skills development.

  1. A Process Analytical Technology (PAT) approach to control a new API manufacturing process: development, validation and implementation.

    PubMed

    Schaefer, Cédric; Clicq, David; Lecomte, Clémence; Merschaert, Alain; Norrant, Edith; Fotiadu, Frédéric

    2014-03-01

    Pharmaceutical companies are progressively adopting and introducing Process Analytical Technology (PAT) and Quality-by-Design (QbD) concepts promoted by the regulatory agencies, aiming the building of the quality directly into the product by combining thorough scientific understanding and quality risk management. An analytical method based on near infrared (NIR) spectroscopy was developed as a PAT tool to control on-line an API (active pharmaceutical ingredient) manufacturing crystallization step during which the API and residual solvent contents need to be precisely determined to reach the predefined seeding point. An original methodology based on the QbD principles was designed to conduct the development and validation of the NIR method and to ensure that it is fitted for its intended use. On this basis, Partial least squares (PLS) models were developed and optimized using chemometrics methods. The method was fully validated according to the ICH Q2(R1) guideline and using the accuracy profile approach. The dosing ranges were evaluated to 9.0-12.0% w/w for the API and 0.18-1.50% w/w for the residual methanol. As by nature the variability of the sampling method and the reference method are included in the variability obtained for the NIR method during the validation phase, a real-time process monitoring exercise was performed to prove its fit for purpose. The implementation of this in-process control (IPC) method on the industrial plant from the launch of the new API synthesis process will enable automatic control of the final crystallization step in order to ensure a predefined quality level of the API. In addition, several valuable benefits are expected including reduction of the process time, suppression of a rather difficult sampling and tedious off-line analyses.

  2. The Rising Cost of Higher Education: Charles Eliot's "Free Money" Strategy and the Beginning of Howard Bowen's "Revenue Theory of Cost," 1869-1979

    ERIC Educational Resources Information Center

    Kimball, Bruce A.

    2014-01-01

    In order to explain the rising cost of higher education, economist Howard Bowen in 1980 proposed his "famous law" of institutional finance. Bowen based his "revenue theory of cost" on a study of aggregate quantitative data extending from 1929 to 1979. Neither he nor subsequent economists asked whether or how that…

  3. Advanced retorting, microwave assisted thermal sterilization (MATS), and pressure assisted thermal sterilization (PATS) to process meat products.

    PubMed

    Barbosa-Cánovas, Gustavo V; Medina-Meza, Ilce; Candoğan, Kezban; Bermúdez-Aguirre, Daniela

    2014-11-01

    Conventional thermal processes have been very reliable in offering safe sterilized meat products, but some of those products are of questionable overall quality. Flavor, aroma, and texture, among other attributes, are significantly affected during such processes. To improve those quality attributes, alternative approaches to sterilizing meat and meat products have been explored in the last few years. Most of the new strategies for sterilizing meat products rely on using thermal approaches, but in a more efficient way than in conventional methods. Some of these emerging technologies have proven to be reliable and have been formally approved by regulatory agencies such as the FDA. Additional work needs to be done in order for these technologies to be fully adopted by the food industry and to optimize their use. Some of these emerging technologies for sterilizing meat include pressure assisted thermal sterilization (PATS), microwaves, and advanced retorting. This review deals with fundamental and applied aspects of these new and very promising approaches to sterilization of meat products.

  4. PAtCh-Cap: input strategy for improving analysis of ChIP-exo data sets and beyond

    PubMed Central

    Terooatea, Tommy W.; Pozner, Amir; Buck-Koehntop, Bethany A.

    2016-01-01

    Recently, a number of advances have been implemented into the core ChIP-seq (chromatin immunoprecipitation coupled with next-generation sequencing) methodology to streamline the process, reduce costs or improve data resolution. Several of these emerging ChIP-based methods perform additional chemical steps on bead-bound immunoprecipitated chromatin, posing a challenge for generating similarly treated input controls required for artifact removal during bioinformatics analyses. Here we present a versatile method for producing technique-specific input controls for ChIP-based methods that utilize additional bead-bound processing steps. This reported method, termed protein attached chromatin capture (PAtCh-Cap), relies on the non-specific capture of chromatin-bound proteins via their carboxylate groups, leaving the DNA accessible for subsequent chemical treatments in parallel with chromatin separately immunoprecipitated for the target protein. Application of this input strategy not only significantly enhanced artifact removal from ChIP-exo data, increasing confidence in peak identification and allowing for de novo motif searching, but also afforded discovery of a novel CTCF binding motif. PMID:27550178

  5. Monitoring ibuprofen-nicotinamide cocrystal formation during solvent free continuous cocrystallization (SFCC) using near infrared spectroscopy as a PAT tool.

    PubMed

    Kelly, A L; Gough, T; Dhumal, R S; Halsey, S A; Paradkar, A

    2012-04-15

    The purpose of this work was to explore NIR spectroscopy as a PAT tool to monitor the formation of ibuprofen and nicotinamide cocrystals during extrusion based solvent free continuous cocrystallization (SFCC). Drug and co-former were gravimetrically fed into a heated co-rotating twin screw extruder to form cocrystals. Real-time process monitoring was performed using a high temperature NIR probe in the extruder die to assess cocrystal content and subsequently compared to off-line powder X-ray diffraction measurements. The effect of processing variables, such as temperature and mixing intensity, on the extent of cocrystal formation was investigated. NIR spectroscopy was sensitive to cocrystal formation with the appearance of new peaks and peak shifts, particularly in the 4800-5200 cm(-1) wave-number region. PXRD confirmed an increased conversion of the mixture into cocrystal with increase in barrel temperature and screw mixing intensity. A decrease in screw rotation speed also provided improved cocrystal yield due to the material experiencing longer residence times within the process. A partial least squares analysis in this region of NIR spectrum correlated well with PXRD data, providing a best fit with cocrystal conversion when a limited range of process conditions were considered, for example a single set temperature. The study suggests that NIR spectroscopy could be used to monitor cocrystal purity on an industrial scale using this continuous, solvent-free process.

  6. Multivariate analysis in the pharmaceutical industry: enabling process understanding and improvement in the PAT and QbD era.

    PubMed

    Ferreira, Ana P; Tobyn, Mike

    2015-01-01

    In the pharmaceutical industry, chemometrics is rapidly establishing itself as a tool that can be used at every step of product development and beyond: from early development to commercialization. This set of multivariate analysis methods allows the extraction of information contained in large, complex data sets thus contributing to increase product and process understanding which is at the core of the Food and Drug Administration's Process Analytical Tools (PAT) Guidance for Industry and the International Conference on Harmonisation's Pharmaceutical Development guideline (Q8). This review is aimed at providing pharmaceutical industry professionals an introduction to multivariate analysis and how it is being adopted and implemented by companies in the transition from "quality-by-testing" to "quality-by-design". It starts with an introduction to multivariate analysis and the two methods most commonly used: principal component analysis and partial least squares regression, their advantages, common pitfalls and requirements for their effective use. That is followed with an overview of the diverse areas of application of multivariate analysis in the pharmaceutical industry: from the development of real-time analytical methods to definition of the design space and control strategy, from formulation optimization during development to the application of quality-by-design principles to improve manufacture of existing commercial products.

  7. A methodology to optimize design pattern context size for higher sensitivity to hotspot detection using pattern association tree (PAT)

    NASA Astrophysics Data System (ADS)

    Somani, Shikha; Pathak, Piyush; Verma, Piyush; Madhavan, Sriram; Capodieci, Luigi

    2015-03-01

    Pattern based design rule checks have emerged as an alternative to the traditional rule based design rule checks in the VLSI verification flow [1]. Typically, the design-process weak-points, also referred as design hotspots, are classified into patterns of fixed size. The size of the pattern defines the radius of influence for the process. These fixed sized patterns are used to search and detect process weak points in new designs without running computationally expensive process simulations. However, both the complexity of the pattern and different kinds of physical processes affect the radii of influence. Therefore, there is a need to determine the optimal pattern radius (size) for efficient hotspot detection. The methodology described here uses a combination of pattern classification and pattern search techniques to create a directed graph, referred to as the Pattern Association Tree (PAT). The pattern association tree is then filtered based on the relevance, sensitivity and context area of each pattern node. The critical patterns are identified by traversing the tree and ranking the patterns. This method has plausible applications in various areas such as process characterization, physical design verification and physical design optimization. Our initial experiments in the area of physical design verification confirm that a pattern deck with the radius optimized for each pattern is significantly more accurate at predicting design hotspots when compared to a conventional deck of fixed sized patterns.

  8. 3D noninvasive, high-resolution imaging using a photoacoustic tomography (PAT) system and rapid wavelength-cycling lasers

    NASA Astrophysics Data System (ADS)

    Sampathkumar, Ashwin; Gross, Daniel; Klosner, Marc; Chan, Gary; Wu, Chunbai; Heller, Donald F.

    2015-05-01

    Globally, cancer is a major health issue as advances in modern medicine continue to extend the human life span. Breast cancer ranks second as a cause of cancer death in women in the United States. Photoacoustic (PA) imaging (PAI) provides high molecular contrast at greater depths in tissue without the use of ionizing radiation. In this work, we describe the development of a PA tomography (PAT) system and a rapid wavelength-cycling Alexandrite laser designed for clinical PAI applications. The laser produces 450 mJ/pulse at 25 Hz to illuminate the entire breast, which eliminates the need to scan the laser source. Wavelength cycling provides a pulse sequence in which the output wavelength repeatedly alternates between 755 nm and 797 nm rapidly within milliseconds. We present imaging results of breast phantoms with inclusions of different sizes at varying depths, obtained with this laser source, a 5-MHz 128-element transducer and a 128-channel Verasonics system. Results include PA images and 3D reconstruction of the breast phantom at 755 and 797 nm, delineating the inclusions that mimic tumors in the breast.

  9. Effects of the Activity of Coprophagous Insects on Greenhouse Gas Emissions from Cattle Dung Pats and Changes in Amounts of Nitrogen, Carbon, and Energy.

    PubMed

    Iwasa, Mitsuhiro; Moki, Yukari; Takahashi, Junichi

    2015-02-01

    Effects of coprophagous insects on greenhouse gas emissions from cattle dung pats were investigated during the initial stage in the decomposition of dung, with accompanying changes in nitrogen, carbon, and energy content. We set up three treatments with adults of Caccobius jessoensis Harold (dung beetle) and larvae of the fly Neomyia cornicina (F.): 1) dung with dung beetles; 2) dung with fly larvae; and 3) dung without insects. In these treatments, the gas flux was measured from air flow exiting the glass containers connected with an in vitro continuous gas analysis system. Total gas fluxes from dung pats with fly larvae were lowest in carbon dioxide (CO2), methane (CH4), and nitrous oxide (N2O). The presence of dung beetles significantly increased CO2 flux from dung, but reduced CH4 flux compared with dung without insects. Fluxes of N2O from dung pats with dung beetles and without insects had distinct peaks at different times after the start of the experiment, while N2O from dung with fly larvae was emitted in extremely low levels throughout the experiment. Carbon (C) content in dung with beetles was significantly lower than that of untreated dung pats designated as fresh dung, whereas that of dung with fly larvae was higher than dung with beetles and without insects. Nitrogen (N) content was significantly lower in dung with fly larvae than the other treatments. Contents of C and N in fly pupae were 35.87 and 8.05%, respectively. During the larval growth of the fly, energy accumulated in the fly body was 2,830 J/g.

  10. ePAT: a simple method to tag adenylated RNA to measure poly(A)-tail length and other 3' RACE applications.

    PubMed

    Jänicke, Amrei; Vancuylenberg, John; Boag, Peter R; Traven, Ana; Beilharz, Traude H

    2012-06-01

    The addition of a poly(A)-tail to the 3' termini of RNA molecules influences stability, nuclear export, and efficiency of translation. In the cytoplasm, dynamic changes in the length of the poly(A)-tail have long been recognized as reflective of the switch between translational silence and activation. Thus, measurement of the poly(A)-tail associated with any given mRNA at steady-state can serve as a surrogate readout of its translation-state. Here, we describe a simple new method to 3'-tag adenylated RNA in total RNA samples using the intrinsic property of Escherichia coli DNA polymerase I to extend an RNA primer using a DNA template. This tag can serve as an anchor for cDNA synthesis and subsequent gene-specific PCR to assess poly(A)-tail length. We call this method extension Poly(A) Test (ePAT). The ePAT approach is as efficient as traditional Ligation-Mediated Poly(A) Test (LM-PAT) assays, avoids problems of internal priming associated with oligo-dT-based methods, and allows for the accurate analysis of both the poly(A)-tail length and alternate 3' UTR usage in 3' RACE applications.

  11. ePAT: A simple method to tag adenylated RNA to measure poly(A)-tail length and other 3′ RACE applications

    PubMed Central

    Jänicke, Amrei; Vancuylenberg, John; Boag, Peter R.; Traven, Ana; Beilharz, Traude H.

    2012-01-01

    The addition of a poly(A)-tail to the 3′ termini of RNA molecules influences stability, nuclear export, and efficiency of translation. In the cytoplasm, dynamic changes in the length of the poly(A)-tail have long been recognized as reflective of the switch between translational silence and activation. Thus, measurement of the poly(A)-tail associated with any given mRNA at steady-state can serve as a surrogate readout of its translation-state. Here, we describe a simple new method to 3′-tag adenylated RNA in total RNA samples using the intrinsic property of Escherichia coli DNA polymerase I to extend an RNA primer using a DNA template. This tag can serve as an anchor for cDNA synthesis and subsequent gene-specific PCR to assess poly(A)-tail length. We call this method extension Poly(A) Test (ePAT). The ePAT approach is as efficient as traditional Ligation-Mediated Poly(A) Test (LM-PAT) assays, avoids problems of internal priming associated with oligo-dT-based methods, and allows for the accurate analysis of both the poly(A)-tail length and alternate 3′ UTR usage in 3′ RACE applications. PMID:22543866

  12. PAT-seq: a method to study the integration of 3′-UTR dynamics with gene expression in the eukaryotic transcriptome

    PubMed Central

    Harrison, Paul F.; Powell, David R.; Clancy, Jennifer L.; Preiss, Thomas; Boag, Peter R.; Traven, Ana; Seemann, Torsten; Beilharz, Traude H.

    2015-01-01

    A major objective of systems biology is to quantitatively integrate multiple parameters from genome-wide measurements. To integrate gene expression with dynamics in poly(A) tail length and adenylation site, we developed a targeted next-generation sequencing approach, Poly(A)-Test RNA-sequencing. PAT-seq returns (i) digital gene expression, (ii) polyadenylation site/s, and (iii) the polyadenylation-state within and between eukaryotic transcriptomes. PAT-seq differs from previous 3′ focused RNA-seq methods in that it depends strictly on 3′ adenylation within total RNA samples and that the full-native poly(A) tail is included in the sequencing libraries. Here, total RNA samples from budding yeast cells were analyzed to identify the intersect between adenylation state and gene expression in response to loss of the major cytoplasmic deadenylase Ccr4. Furthermore, concordant changes to gene expression and adenylation-state were demonstrated in the classic Crabtree–Warburg metabolic shift. Because all polyadenylated RNA is interrogated by the approach, alternative adenylation sites, noncoding RNA and RNA-decay intermediates were also identified. Most important, the PAT-seq approach uses standard sequencing procedures, supports significant multiplexing, and thus replication and rigorous statistical analyses can for the first time be brought to the measure of 3′-UTR dynamics genome wide. PMID:26092945

  13. Psychology, not educational neuroscience, is the way forward for improving educational outcomes for all children: Reply to Gabrieli (2016) and Howard-Jones et al. (2016).

    PubMed

    Bowers, Jeffrey S

    2016-10-01

    In Bowers (2016), I argued that there are (a) practical problems with educational neuroscience (EN) that explain why there are no examples of EN improving teaching and (b) principled problems with the logic motivating EN that explain why it is likely that there never will be. In the following article, I consider the main responses raised by both Gabrieli (2016) and Howard-Jones et al. (2016) and find them all unconvincing. Following this exchange, there are still no examples of EN providing new insights to teaching in the classroom, there are still no examples of EN providing new insights to remedial instructions for individuals, and, as I detail in this article, there is no evidence that EN is useful for the diagnosis of learning difficulties. The authors have also failed to address the reasons why EN is unlikely to benefit educational outcomes in the future. Psychology, by contrast, can (and has) made important discoveries that can (and should) be used to improve teaching and diagnostic tests for learning difficulties. This is not a debate about whether science is relevant to education, rather it is about what sort of science is relevant. (PsycINFO Database Record

  14. Psychological keys in the study of African American religious folk songs in the early work of Howard W. Odum (1884-1954).

    PubMed

    Bernal-Marcos, Marcos José; Castro-Tejerina, Jorge; Loredo-Narciandi, José Carlos

    2017-02-01

    This study focuses on the analysis of the early work of Howard W. Odum (1884-1954) and the examination of the psychological aspects that marked his reflection on African American music. This analysis reveals many of the aspects that were generically shared by the psychological agenda of the period when analyzing aesthetic experience and activity. Outstanding among these are the relationship of the musical phenomenon with very basic or primary affective-emotional dimensions, the conception of the musical phenomenon as an indicator of the cognitive-affective development of human groups, its expression in the form of cultural and complex intersubjective products, or its possible participation in the technoscientific design of social reform and progress. The simultaneous treatment of all of these aspects in Odum's work brings to light the interdisciplinary framework in which early psychology moved, while revealing the theoretical and ideological contradictions and controversies that enveloped the discipline, above all, at the point where it attempted to place itself at the service of the constitution of self-governed individuals. All in all, Odum's work also reflects the crucial role that early psychology attributed to art as a privileged medium to give meaning to experience and the human being's vital purposes. (PsycINFO Database Record

  15. 'DIRTY WORK', BUT SOMEONE HAS TO DO IT: HOWARD P. ROBERTSON AND THE REFEREEING PRACTICES OF PHYSICAL REVIEW IN THE 1930S.

    PubMed

    Lalli, Roberto

    2016-06-20

    In the 1930s the mathematical physicist Howard P. Robertson was the main referee of the journal Physical Review for papers concerning general relativity and related subjects. The rich correspondence between Robertson and the editors of the journal enables a historical investigation of the refereeing process of Physical Review at the time that it was becoming one of the most influential physics periodicals in the world. By focusing on this case study, the paper investigates two complementary aspects of the evolution of the refereeing process: first, the historical evolution of the refereeing practices in connection with broader contextual changes, and second, the attempts to define the activity of the referee, including the epistemic virtues required and the journal's functions according to the participants' categories. By exploring the tension between Robertson's idealized picture about how the referee should behave and the desire to promote his intellectual agenda, I show that the evaluation criteria that Robertson employed were contextually dependent and I argue that, in the 1930s, through his reports the referee had an enormous power in defining what direction future research should take.

  16. Revolutionary changes in medicine and ophthalmology--the St. Louis, MO, effect: Homer G. Phillips Hospital and Dr. Howard Phillip venable.

    PubMed Central

    Johnson, Andrew S.

    2003-01-01

    PURPOSE: To provide information on the revolutionary changes in medicine, particularly on the development of African-Americans in ophthalmology, created by Homer G. Phillips Hospital in St. Louis and the late Dr. Howard Phillip Venable. METHODS: Very little has been written about Homer G. Phillips Hospital and Dr. H. Phillip Venable. Through personal interviews with physicians trained by Dr. Venable and literature review, I was able to obtain information on Homer G. Phillips Hospital and Dr. Venable's influence in breaking the color line in medicine and ophthalmology. RESULTS: Homer G. Phillips Hospital in St. Louis was a major teaching hospital for African-American doctors. Dr. Venable was one of a few African-American ophthalmologists in the 1940s. Dr. Venable then trained approximately 40 African-American ophthalmologists at Homer G. Phillips Hospital between 1943 to 1979, when the hospital closed. CONCLUSION: The ophthalmologists trained by Dr. Venable have gone forward to treat thousands of patients with blinding eye diseases such as glaucoma and diabetes, and to influence others to become ophthalmologists. Although the hospital has closed and Dr. Venable has passed away, their existence has reformed the medical treatment received by African-Americans and other people. Images p484-a PMID:12856914

  17. Real-time determination of critical quality attributes using near-infrared spectroscopy: a contribution for Process Analytical Technology (PAT).

    PubMed

    Rosas, Juan G; Blanco, Marcel; González, Josep M; Alcalà, Manel

    2012-08-15

    Process Analytical Technology (PAT) is playing a central role in current regulations on pharmaceutical production processes. Proper understanding of all operations and variables connecting the raw materials to end products is one of the keys to ensuring quality of the products and continuous improvement in their production. Near infrared spectroscopy (NIRS) has been successfully used to develop faster and non-invasive quantitative methods for real-time predicting critical quality attributes (CQA) of pharmaceutical granulates (API content, pH, moisture, flowability, angle of repose and particle size). NIR spectra have been acquired from the bin blender after granulation process in a non-classified area without the need of sample withdrawal. The methodology used for data acquisition, calibration modelling and method application in this context is relatively inexpensive and can be easily implemented by most pharmaceutical laboratories. For this purpose, Partial Least-Squares (PLS) algorithm was used to calculate multivariate calibration models, that provided acceptable Root Mean Square Error of Predictions (RMSEP) values (RMSEP(API)=1.0 mg/g; RMSEP(pH)=0.1; RMSEP(Moisture)=0.1%; RMSEP(Flowability)=0.6 g/s; RMSEP(Angle of repose)=1.7° and RMSEP(Particle size)=2.5%) that allowed the application for routine analyses of production batches. The proposed method affords quality assessment of end products and the determination of important parameters with a view to understanding production processes used by the pharmaceutical industry. As shown here, the NIRS technique is a highly suitable tool for Process Analytical Technologies.

  18. PAT-Based Control of Fluid Bed Coating Process Using NIR Spectroscopy to Monitor the Cellulose Coating on Pharmaceutical Pellets.

    PubMed

    Naidu, Venkata Ramana; Deshpande, Rucha S; Syed, Moinuddin R; Deoghare, Piyush; Singh, Dharamvir; Wakte, Pravin S

    2016-12-19

    Current endeavor was aimed towards monitoring percent weight build-up during functional coating process on drug-layered pellets. Near-infrared (NIR) spectroscopy is an emerging process analytical technology (PAT) tool which was employed here within quality by design (QbD) framework. Samples were withdrawn after spraying every 15-Kg cellulosic coating material during Wurster coating process of drug-loaded pellets. NIR spectra of these samples were acquired using cup spinner assembly of Thermoscientific Antaris II, followed by multivariate analysis using partial least squares (PLS) calibration model. PLS model was built by selecting various absorption regions of NIR spectra for Ethyl cellulose, drug and correlating the absorption values with actual percent weight build up determined by HPLC. The spectral regions of 8971.04 to 8250.77 cm(-1), 7515.24 to 7108.33 cm(-1), and 5257.00 to 5098.87 cm(-1) were found to be specific to cellulose, where as the spectral region of 6004.45 to 5844.14 cm(-1)was found to be specific to drug. The final model gave superb correlation co-efficient value of 0.9994 for calibration and 0.9984 for validation with low root mean square of error (RMSE) values of 0.147 for calibration and 0.371 for validation using 6 factors. The developed correlation between the NIR spectra and cellulose content is useful in precise at-line prediction of functional coat value and can be used for monitoring the Wurster coating process.

  19. Introducing process analytical technology (PAT) in filamentous cultivation process development: comparison of advanced online sensors for biomass measurement.

    PubMed

    Rønnest, Nanna Petersen; Stocks, Stuart M; Eliasson Lantz, Anna; Gernaey, Krist V

    2011-10-01

    The recent process analytical technology (PAT) initiative has put an increased focus on online sensors to generate process-relevant information in real time. Specifically for fermentation, however, introduction of online sensors is often far from straightforward, and online measurement of biomass is one of the best examples. The purpose of this study was therefore to compare the performance of various online biomass sensors, and secondly to demonstrate their use in early development of a filamentous cultivation process. Eight Streptomyces coelicolor fed-batch cultivations were run as part of process development in which the pH, the feeding strategy, and the medium composition were varied. The cultivations were monitored in situ using multi-wavelength fluorescence (MWF) spectroscopy, scanning dielectric (DE) spectroscopy, and turbidity measurements. In addition, we logged all of the classical cultivation data, such as the carbon dioxide evolution rate (CER) and the concentration of dissolved oxygen. Prediction models for the biomass concentrations were estimated on the basis of the individual sensors and on combinations of the sensors. The results showed that the more advanced sensors based on MWF and scanning DE spectroscopy did not offer any advantages over the simpler sensors based on dual frequency DE spectroscopy, turbidity, and CER measurements for prediction of biomass concentration. By combining CER, DE spectroscopy, and turbidity measurements, the prediction error was reduced to 1.5 g/l, corresponding to 6% of the covered biomass range. Moreover, by using multiple sensors it was possible to check the quality of the individual predictions and switch between the sensors in real time.

  20. Re-Os systematics and age of pyrite associated with stratiform Zn-Pb mineralization in the Howards Pass district, Yukon and Northwest Territories, Canada

    NASA Astrophysics Data System (ADS)

    Kelley, Karen D.; Selby, David; Falck, Hendrik; Slack, John F.

    2016-05-01

    Stratiform Zn-Pb deposits hosted in unmetamorphosed carbonaceous and siliceous mudstones of the Ordovician to Silurian Duo Lake Formation define the Howards Pass district in Yukon Territory and Northwest Territories, western Canada. Collectively, the deposits are amongst the largest in the world, containing drill-indicated and inferred resources of 423 Mt at 4.84 % Zn and 1.59 % Pb. Sulphide textures include (a) fine-scale laminations of sphalerite, galena, and pyrite from <0.05 mm to 1 cm thick, interbedded with carbonaceous sedimentary rock; (b) layers of coarse sulphide that are structurally controlled by microfolds; and (c) veins that cut bedding and sulphide laminations. The finely interlaminated nature of sulphides with mudstone has been used as evidence for syngenetic mineralizing processes, whereas paleomagnetic data determined on coarse layered sulphides suggest a Middle Jurassic age of mineralization. Here, we present new rhenium-osmium (Re-Os) isotopic data for 12 pyrite separates obtained from 4 laminated sulphide-rich samples from the XY Central (XYC) and Don (DON) deposits and for 1 unmineralized organic-rich mudstone ˜20 m stratigraphically below the sulphide-bearing zone. Pyrite separates that lack mudstone inclusions ("pure") from the XYC deposit contain 2.2 to 4.0 ppb Re and 93.4 to 123.4 ppt Os; pure pyrite from the DON deposit is significantly more enriched in Re and Os (34-37 ppb Re; 636.8-694.9 ppt Os). The 187Re/188Os values of pure pyrite separates from the XYC and DON deposits range from 137.6 to 197 and 182.1 to 201.4, respectively. Regression of all pure pyrite Re-Os data from both deposits yields an isochron age of 442 ± 14 Ma (MSWD = 7.4) and an initial 187Os/188Os (Osi) value of 0.71 ± 0.07. The Re-Os age indicates that the early phase of pyrite precipitation (and by inference, sphalerite and galena) occurred during the early Silurian, consistent with biostratigraphic ages of the host rocks. The Osi value of ˜0.8 for earliest

  1. Re-Os systematics and age of pyrite associated with stratiform Zn-Pb mineralization in the Howards Pass district, Yukon and Northwest Territories, Canada

    NASA Astrophysics Data System (ADS)

    Kelley, Karen D.; Selby, David; Falck, Hendrik; Slack, John F.

    2017-03-01

    Stratiform Zn-Pb deposits hosted in unmetamorphosed carbonaceous and siliceous mudstones of the Ordovician to Silurian Duo Lake Formation define the Howards Pass district in Yukon Territory and Northwest Territories, western Canada. Collectively, the deposits are amongst the largest in the world, containing drill-indicated and inferred resources of 423 Mt at 4.84 % Zn and 1.59 % Pb. Sulphide textures include (a) fine-scale laminations of sphalerite, galena, and pyrite from <0.05 mm to 1 cm thick, interbedded with carbonaceous sedimentary rock; (b) layers of coarse sulphide that are structurally controlled by microfolds; and (c) veins that cut bedding and sulphide laminations. The finely interlaminated nature of sulphides with mudstone has been used as evidence for syngenetic mineralizing processes, whereas paleomagnetic data determined on coarse layered sulphides suggest a Middle Jurassic age of mineralization. Here, we present new rhenium-osmium (Re-Os) isotopic data for 12 pyrite separates obtained from 4 laminated sulphide-rich samples from the XY Central (XYC) and Don (DON) deposits and for 1 unmineralized organic-rich mudstone ˜20 m stratigraphically below the sulphide-bearing zone. Pyrite separates that lack mudstone inclusions ("pure") from the XYC deposit contain 2.2 to 4.0 ppb Re and 93.4 to 123.4 ppt Os; pure pyrite from the DON deposit is significantly more enriched in Re and Os (34-37 ppb Re; 636.8-694.9 ppt Os). The 187Re/188Os values of pure pyrite separates from the XYC and DON deposits range from 137.6 to 197 and 182.1 to 201.4, respectively. Regression of all pure pyrite Re-Os data from both deposits yields an isochron age of 442 ± 14 Ma (MSWD = 7.4) and an initial 187Os/188Os (Osi) value of 0.71 ± 0.07. The Re-Os age indicates that the early phase of pyrite precipitation (and by inference, sphalerite and galena) occurred during the early Silurian, consistent with biostratigraphic ages of the host rocks. The Osi value of ˜0.8 for earliest

  2. Geochemistry of host rocks in the Howards Pass district, Yukon-Northwest Territories, Canada: implications for sedimentary environments of Zn-Pb and phosphate mineralization

    NASA Astrophysics Data System (ADS)

    Slack, John F.; Falck, Hendrik; Kelley, Karen D.; Xue, Gabriel G.

    2016-10-01

    Detailed lithogeochemical data are reported here on early Paleozoic sedimentary rocks that host the large Howards Pass stratiform Zn-Pb deposits in Yukon-Northwest Territories. Redox-sensitive trace elements (Mo, Re, V, U) and Ce anomalies in members of the Duo Lake Formation record significant environmental changes. During the deposition of lower footwall units (Pyritic siliceous and Calcareous mudstone members), bottom waters were anoxic and sulphidic, respectively; these members formed in a marginal basin that may have become increasingly restricted with time. Relative to lower members, a major environmental change is proposed for deposition of the overlying Lower cherty mudstone member, which contains phosphorite beds up to ˜0.8 m thick in the upper part, near the base of the Zn-Pb deposits. The presence of these beds, together with models for modern phosphorite formation, suggests P input from an upwelling system and phosphorite deposition in an upper slope or outer shelf setting. The overlying Active mudstone member contains stratabound to stratiform Zn-Pb deposits within black mudstone and gray calcareous mudstone. Data for unmineralized black mudstone in this member indicate deposition under diverse redox conditions from suboxic to sulphidic. Especially distinctive in this member are uniformly low ratios of light to heavy rare earth elements that are unique within the Duo Lake Formation, attributed here to the dissolution of sedimentary apatite by downward-percolating acidic metalliferous brines. Strata that overlie the Active member (Upper siliceous mudstone member) consist mainly of black mudstone with thin (0.5-1.5 cm) laminae of fine-grained apatite, recording continued deposition on an upper slope or outer shelf under predominantly suboxic bottom waters. Results of this study suggest that exploration for similar stratiform sediment-hosted Zn-Pb deposits should include the outer parts of ancient continental margins, especially at and near stratigraphic

  3. Geochemistry of host rocks in the Howards Pass district, Yukon-Northwest Territories, Canada: implications for sedimentary environments of Zn-Pb and phosphate mineralization

    USGS Publications Warehouse

    Slack, John F.; Falck, Hendrik; Kelley, Karen D.; Xue, Gabriel G.

    2017-01-01

    Detailed lithogeochemical data are reported here on early Paleozoic sedimentary rocks that host the large Howards Pass stratiform Zn-Pb deposits in Yukon-Northwest Territories. Redox-sensitive trace elements (Mo, Re, V, U) and Ce anomalies in members of the Duo Lake Formation record significant environmental changes. During the deposition of lower footwall units (Pyritic siliceous and Calcareous mudstone members), bottom waters were anoxic and sulphidic, respectively; these members formed in a marginal basin that may have become increasingly restricted with time. Relative to lower members, a major environmental change is proposed for deposition of the overlying Lower cherty mudstone member, which contains phosphorite beds up to ∼0.8 m thick in the upper part, near the base of the Zn-Pb deposits. The presence of these beds, together with models for modern phosphorite formation, suggests P input from an upwelling system and phosphorite deposition in an upper slope or outer shelf setting. The overlying Active mudstone member contains stratabound to stratiform Zn-Pb deposits within black mudstone and gray calcareous mudstone. Data for unmineralized black mudstone in this member indicate deposition under diverse redox conditions from suboxic to sulphidic. Especially distinctive in this member are uniformly low ratios of light to heavy rare earth elements that are unique within the Duo Lake Formation, attributed here to the dissolution of sedimentary apatite by downward-percolating acidic metalliferous brines. Strata that overlie the Active member (Upper siliceous mudstone member) consist mainly of black mudstone with thin (0.5–1.5 cm) laminae of fine-grained apatite, recording continued deposition on an upper slope or outer shelf under predominantly suboxic bottom waters. Results of this study suggest that exploration for similar stratiform sediment-hosted Zn-Pb deposits should include the outer parts of ancient continental margins, especially at and near

  4. On-line near infrared spectroscopy as a Process Analytical Technology (PAT) tool to control an industrial seeded API crystallization.

    PubMed

    Schaefer, C; Lecomte, C; Clicq, D; Merschaert, A; Norrant, E; Fotiadu, F

    2013-09-01

    The final step of an active pharmaceutical ingredient (API) manufacturing synthesis process consists of a crystallization during which the API and residual solvent contents have to be quantified precisely in order to reach a predefined seeding point. A feasibility study was conducted to demonstrate the suitability of on-line NIR spectroscopy to control this step in line with new version of the European Medicines Agency (EMA) guideline [1]. A quantitative method was developed at laboratory scale using statistical design of experiments (DOE) and multivariate data analysis such as principal component analysis (PCA) and partial least squares (PLS) regression. NIR models were built to quantify the API in the range of 9-12% (w/w) and to quantify the residual methanol in the range of 0-3% (w/w). To improve the predictive ability of the models, the development procedure encompassed: outliers elimination, optimum model rank definition, spectral range and spectral pre-treatment selection. Conventional criteria such as, number of PLS factors, R(2), root mean square errors of calibration, cross-validation and prediction (RMSEC, RMSECV, RMSEP) enabled the selection of three model candidates. These models were tested in the industrial pilot plant during three technical campaigns. Results of the most suitable models were evaluated against to the chromatographic reference methods. Maximum relative bias of 2.88% was obtained about API target content. Absolute bias of 0.01 and 0.02% (w/w) respectively were achieved at methanol content levels of 0.10 and 0.13% (w/w). The repeatability was assessed as sufficient for the on-line monitoring of the 2 analytes. The present feasibility study confirmed the possibility to use on-line NIR spectroscopy as a PAT tool to monitor in real-time both the API and the residual methanol contents, in order to control the seeding of an API crystallization at industrial scale. Furthermore, the successful scale-up of the method proved its capability to be

  5. Constitutive expression of McCHIT1-PAT enhances resistance to rice blast and herbicide, but does not affect grain yield in transgenic glutinous rice.

    PubMed

    Zeng, Xiao-Fang; Li, Lei; Li, Jian-Rong; Zhao, De-Gang

    2016-01-01

    To produce new rice blast- and herbicide-resistant transgenic rice lines, the McCHIT1 gene encoding the class I chitinase from Momordica charantia and the herbicide resistance gene PAT were introduced into Lailong (Oryza sativa L. ssp. Japonica), a glutinous local rice variety from Guizhou Province, People's Republic of China. Transgenic lines were identified by ß-glucuronidase (GUS) histochemical staining, PCR, and Southern blot analyses. Agronomic traits, resistance to rice blast and herbicide, chitinase activities, and transcript levels of McCHIT1 were assessed in the T2 progeny of three transgenic lines (L1, L8, and L10). The results showed that the introduction of McCHIT1-PAT into Lailong significantly enhanced herbicide and blast resistance. After infection with the blast fungus Magnaporthe oryzae, all of the T2 progeny exhibited less severe lesion symptoms than those of wild type. The disease indices were 100% for wild type, 65.66% for T2 transgenic line L1, 59.69% for T2 transgenic line L8, and 79.80% for T2 transgenic line L10. Transgenic lines expressing McCHIT1-PAT did not show a significant difference from wild type in terms of malondialdehyde (MDA) content, polyphenol oxidase (PPO) activity, and superoxide dismutase (SOD) activity in the leaves. However, after inoculation with M. oryzae, transgenic plants showed significantly higher SOD and PPO activities and lower MDA contents in leaves, compared with those in wild-type leaves. The transgenic and the wild-type plants did not show significant differences in grain yield parameters including plant height, panicles per plant, seeds per panicle, and 1000-grain weight. Therefore, the transgenic plants showed increased herbicide and blast resistance, with no yield penalty.

  6. Biomanufacturing process analytical technology (PAT) application for downstream processing: Using dissolved oxygen as an indicator of product quality for a protein refolding reaction.

    PubMed

    Pizarro, Shelly A; Dinges, Rachel; Adams, Rachel; Sanchez, Ailen; Winter, Charles

    2009-10-01

    Process analytical technology (PAT) is an initiative from the US FDA combining analytical and statistical tools to improve manufacturing operations and ensure regulatory compliance. This work describes the use of a continuous monitoring system for a protein refolding reaction to provide consistency in product quality and process performance across batches. A small-scale bioreactor (3 L) is used to understand the impact of aeration for refolding recombinant human vascular endothelial growth factor (rhVEGF) in a reducing environment. A reverse-phase HPLC assay is used to assess product quality. The goal in understanding the oxygen needs of the reaction and its impact to quality, is to make a product that is efficiently refolded to its native and active form with minimum oxidative degradation from batch to batch. Because this refolding process is heavily dependent on oxygen, the % dissolved oxygen (DO) profile is explored as a PAT tool to regulate process performance at commercial manufacturing scale. A dynamic gassing out approach using constant mass transfer (k(L)a) is used for scale-up of the aeration parameters to manufacturing scale tanks (2,000 L, 15,000 L). The resulting DO profiles of the refolding reaction show similar trends across scales and these are analyzed using rpHPLC. The desired product quality attributes are then achieved through alternating air and nitrogen sparging triggered by changes in the monitored DO profile. This approach mitigates the impact of differences in equipment or feedstock components between runs, and is directly inline with the key goal of PAT to "actively manage process variability using a knowledge-based approach."

  7. Pop-up archival transmitting (PAT) tags: A method to investigate the migration and behavior of Pacific halibut (Hippoglossus stenolepis) in the Gulf of Alaska

    USGS Publications Warehouse

    Seitz, Andrew C; Wilson, Derek; Norcross, Brenda L.; Nielsen, Jennifer L.

    2003-01-01

    Pop-up archival transmitting (PAT) tags provide a fisheries-independent method of collecting environmental preference data (depth and ambient water temperature) and migration distance. In this study, we evaluate the use of pop-up archival transmitting tags as a method to investigate demersal fish. We report the results from eight pop-up archival transmitting tagged Pacific halibut Hippoglossus stenolepis (from 107 to 165 cm FL) that were released in and around Resurrection Bay, Alaska. Commercial fishermen recovered three tags, while five tags transmitted data to Argos satellites. Horizontal migration was not consistent among fish as four Pacific halibut remained in the vicinity of release while the other four traveled up to 358 km from the release site. Vertical movement was not consistent among fish or over time; however, they spent most of their time at depths of 150 to 350 m. The minimum and maximum depths reached by any of the Pacific halibut were 2 m and 502 m, respectively. The fish preferred water temperatures of approximately 6°C, but experienced temperatures between 4.3 and 12.2°C. Light attenuation with depth prevented geolocation software and light sensing hardware from accurately estimating geoposition for the majority of days. The methods, adapted from investigations on large pelagic fish, proved to be effective for studying Pacific halibut in the northern Gulf of Alaska. PAT tags allowed us to obtain high accuracy locations of the fish at the end of the tag deployments as well as preliminary data to identify approximate seasonal locations and to characterize their depth and temperature characteristics. By using PAT tags, we will be able to ensure tag returns during the winter season (which is closed to fishing) and gain valuable biological information even if fish migrate large distances or to unexpected locations.

  8. The world-class Howard's Pass SEDEX Zn-Pb district, Selwyn Basin, Yukon. Part II: the roles of thermochemical and bacterial sulfate reduction in metal fixation

    NASA Astrophysics Data System (ADS)

    Gadd, Michael G.; Layton-Matthews, Daniel; Peter, Jan M.; Paradis, Suzanne; Jonasson, Ian R.

    2016-07-01

    The Howard's Pass district of sedimentary exhalative (SEDEX) Zn-Pb deposits is located in Yukon Territory and comprises 14 Zn-Pb deposits that contain an estimated 400.7 Mt of sulfide mineralization grading 4.5 % Zn and 1.5 % Pb. Mineralization is hosted in carbonaceous and calcareous and, to a lesser extent, siliceous mudstones. Pyrite is a minor but ubiquitous mineral in the host rocks stratigraphically above, within, and below mineralization. Petrographic analyses reveal that pyrite has a complex and protracted growth history, preserving multiple generations of pyrite within single grains. Sulfur isotope analysis of paragenetically complex pyrite by secondary ion mass spectrometry (SIMS) reveals that sulfur isotope compositions vary with textural zonation. Within the Zn-Pb deposits, framboidal pyrite is the earliest pyrite generation recognized, and this exclusively has negative δ34S values (mean = -16.6 ± 4.1 ‰; n = 55), whereas paragenetically later pyrite and galena possess positive δ34S values (mean = 29.1 ± 7.5 and 22.4 ± 3.0 ‰, n = 13 and 13, respectively). Previous studies found that sphalerite and galena mineral separates have exclusively positive δ34S values (mean = 16.8 ± 3.3 and 12.7 ± 2.8 ‰, respectively; Goodfellow and Jonasson 1986). These distinct sulfur isotope values are interpreted to reflect varying contributions of bacterially reduced seawater sulfate (negative; framboidal pyrite) and thermochemically reduced seawater sulfate and/or hydrothermal sulfate (positive; galena, sphalerite, later forms of pyrite). Textural evidence indicates that framboidal pyrite predates galena and sphalerite deposition. Collectively, the in situ and bulk sulfur isotope data are much more complex than δ34S values permitted by prevailing genetic models that invoke only biogenically reduced sulfur and coeval deposition of galena, sphalerite, and framboidal pyrite within a euxinic water column, and we present several lines of evidence that argue

  9. The world-class Howard's Pass SEDEX Zn-Pb district, Selwyn Basin, Yukon. Part II: the roles of thermochemical and bacterial sulfate reduction in metal fixation

    NASA Astrophysics Data System (ADS)

    Gadd, Michael G.; Layton-Matthews, Daniel; Peter, Jan M.; Paradis, Suzanne; Jonasson, Ian R.

    2017-03-01

    The Howard's Pass district of sedimentary exhalative (SEDEX) Zn-Pb deposits is located in Yukon Territory and comprises 14 Zn-Pb deposits that contain an estimated 400.7 Mt of sulfide mineralization grading 4.5 % Zn and 1.5 % Pb. Mineralization is hosted in carbonaceous and calcareous and, to a lesser extent, siliceous mudstones. Pyrite is a minor but ubiquitous mineral in the host rocks stratigraphically above, within, and below mineralization. Petrographic analyses reveal that pyrite has a complex and protracted growth history, preserving multiple generations of pyrite within single grains. Sulfur isotope analysis of paragenetically complex pyrite by secondary ion mass spectrometry (SIMS) reveals that sulfur isotope compositions vary with textural zonation. Within the Zn-Pb deposits, framboidal pyrite is the earliest pyrite generation recognized, and this exclusively has negative δ34S values (mean = -16.6 ± 4.1 ‰; n = 55), whereas paragenetically later pyrite and galena possess positive δ34S values (mean = 29.1 ± 7.5 and 22.4 ± 3.0 ‰, n = 13 and 13, respectively). Previous studies found that sphalerite and galena mineral separates have exclusively positive δ34S values (mean = 16.8 ± 3.3 and 12.7 ± 2.8 ‰, respectively; Goodfellow and Jonasson 1986). These distinct sulfur isotope values are interpreted to reflect varying contributions of bacterially reduced seawater sulfate (negative; framboidal pyrite) and thermochemically reduced seawater sulfate and/or hydrothermal sulfate (positive; galena, sphalerite, later forms of pyrite). Textural evidence indicates that framboidal pyrite predates galena and sphalerite deposition. Collectively, the in situ and bulk sulfur isotope data are much more complex than δ34S values permitted by prevailing genetic models that invoke only biogenically reduced sulfur and coeval deposition of galena, sphalerite, and framboidal pyrite within a euxinic water column, and we present several lines of evidence that argue

  10. Quality-by-Design (QbD): An integrated process analytical technology (PAT) approach for a dynamic pharmaceutical co-precipitation process characterization and process design space development.

    PubMed

    Wu, Huiquan; White, Maury; Khan, Mansoor A

    2011-02-28

    The aim of this work was to develop an integrated process analytical technology (PAT) approach for a dynamic pharmaceutical co-precipitation process characterization and design space development. A dynamic co-precipitation process by gradually introducing water to the ternary system of naproxen-Eudragit L100-alcohol was monitored at real-time in situ via Lasentec FBRM and PVM. 3D map of count-time-chord length revealed three distinguishable process stages: incubation, transition, and steady-state. The effects of high risk process variables (slurry temperature, stirring rate, and water addition rate) on both derived co-precipitation process rates and final chord-length-distribution were evaluated systematically using a 3(3) full factorial design. Critical process variables were identified via ANOVA for both transition and steady state. General linear models (GLM) were then used for parameter estimation for each critical variable. Clear trends about effects of each critical variable during transition and steady state were found by GLM and were interpreted using fundamental process principles and Nyvlt's transfer model. Neural network models were able to link process variables with response variables at transition and steady state with R(2) of 0.88-0.98. PVM images evidenced nucleation and crystal growth. Contour plots illustrated design space via critical process variables' ranges. It demonstrated the utility of integrated PAT approach for QbD development.

  11. Increased heterocyst frequency by patN disruption in Anabaena leads to enhanced photobiological hydrogen production at high light intensity and high cell density.

    PubMed

    Masukawa, Hajime; Sakurai, Hidehiro; Hausinger, Robert P; Inoue, Kazuhito

    2017-03-01

    The effects of increasing the heterocyst-to-vegetative cell ratio on the nitrogenase-based photobiological hydrogen production by the filamentous heterocyst-forming cyanobacterium Anabaena sp. PCC 7120 were studied. Using the uptake hydrogenase-disrupted mutant (ΔHup) as the parent, a deletion-insertion mutant (PN1) was created in patN, known to be involved in heterocyst pattern formation and leading to multiple singular heterocysts (MSH) in Nostoc punctiforme strain ATCC 29133. The PN1 strain showed heterocyst differentiation but failed to grow in medium free of combined-nitrogen; however, a spontaneous mutant (PN22) was obtained on prolonged incubation of PN1 liquid cultures and was able to grow robustly on N2. The disruption of patN was confirmed in both PN1 and PN22 by PCR and whole genome resequencing. Under combined-nitrogen limitation, the percentage of heterocysts to total cells in the PN22 filaments was 13-15 and 16-18% under air and 1% CO2-enriched air, respectively, in contrast to the parent ΔHup which formed 6.5-11 and 9.7-13% heterocysts in these conditions. The PN22 strain exhibited a MSH phenotype, normal diazotrophic growth, and higher H2 productivity at high cell concentrations, and was less susceptible to photoinhibition by strong light than the parent ΔHup strain, resulting in greater light energy utilization efficiency in H2 production on a per unit area basis under high light conditions. The increase in MSH frequency shown here appears to be a viable strategy for enhancing H2 productivity by outdoor cultures of cyanobacteria in high-light environments.

  12. An integrated process analytical technology (PAT) approach to monitoring the effect of supercooling on lyophilization product and process parameters of model monoclonal antibody formulations.

    PubMed

    Awotwe-Otoo, David; Agarabi, Cyrus; Khan, Mansoor A

    2014-07-01

    The aim of the present study was to apply an integrated process analytical technology (PAT) approach to control and monitor the effect of the degree of supercooling on critical process and product parameters of a lyophilization cycle. Two concentrations of a mAb formulation were used as models for lyophilization. ControLyo™ technology was applied to control the onset of ice nucleation, whereas tunable diode laser absorption spectroscopy (TDLAS) was utilized as a noninvasive tool for the inline monitoring of the water vapor concentration and vapor flow velocity in the spool during primary drying. The instantaneous measurements were then used to determine the effect of the degree of supercooling on critical process and product parameters. Controlled nucleation resulted in uniform nucleation at lower degrees of supercooling for both formulations, higher sublimation rates, lower mass transfer resistance, lower product temperatures at the sublimation interface, and shorter primary drying times compared with the conventional shelf-ramped freezing. Controlled nucleation also resulted in lyophilized cakes with more elegant and porous structure with no visible collapse or shrinkage, lower specific surface area, and shorter reconstitution times compared with the uncontrolled nucleation. Uncontrolled nucleation however resulted in lyophilized cakes with relatively lower residual moisture contents compared with controlled nucleation. TDLAS proved to be an efficient tool to determine the endpoint of primary drying. There was good agreement between data obtained from TDLAS-based measurements and SMART™ technology. ControLyo™ technology and TDLAS showed great potential as PAT tools to achieve enhanced process monitoring and control during lyophilization cycles.

  13. Kagami-Ogata syndrome: a clinically recognizable upd(14)pat and related disorder affecting the chromosome 14q32.2 imprinted region.

    PubMed

    Ogata, Tsutomu; Kagami, Masayo

    2016-02-01

    Human chromosome 14q32.2 carries paternally expressed genes including DLK1 and RTL1, and maternally expressed genes including MEG3 and RTL1as, along with the germline-derived DLK1-MEG3 intergenic differentially methylated region (IG-DMR) and the postfertilization-derived MEG3-DMR. Consistent with this, paternal uniparental disomy 14 (upd(14)pat), and epimutations (hypermethylations) and microdeletions affecting the IG-DMR and/or the MEG3-DMR of maternal origin, result in a unique phenotype associated with characteristic face, a small bell-shaped thorax with coat-hanger appearance of the ribs, abdominal wall defects, placentomegaly and polyhydramnios. Recently, the name 'Kagami-Ogata syndrome' (KOS) has been approved for this clinically recognizable disorder. Here, we review the current knowledge about KOS. Important findings include the following: (1) the facial 'gestalt' and the increased coat-hanger angle constitute pathognomonic features from infancy through childhood/puberty; (2) the unmethylated IG-DMR and MEG3-DMR of maternal origin function as the imprinting control centers in the placenta and body respectively, with a hierarchical interaction regulated by the IG-DMR for the methylation pattern of the MEG3-DMR in the body; (3) RTL1 expression level becomes ~2.5 times increased in the absence of functional RTL1as-encoded microRNAs that act as a trans-acting repressor for RTL1; (4) excessive RTL1 expression and absent MEG expression constitute the primary underlying factor for the phenotypic development; and (5) upd(14)pat accounts for approximately two-thirds of KOS patients, and epimutations and microdeletions are identified with a similar frequency. Furthermore, we refer to diagnostic and therapeutic implications.

  14. CaMKII-MEDIATED PHOSPHORYLATION OF THE BOMBYX MORI LIPID STORAGE DROPLET PROTEIN-1 (BmLsd1), AN INSECT PAT FAMILY PROTEIN, IS ESSENTIAL FOR SILKMOTH SEX PHEROMONE BIOSYNTHESIS

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The structurally-related members of the PAT family of proteins, which are so name based on similarity amongst perilipin, adipophilin/adipocyte differentiation-related protein (ADRP), and tail-interacting protein of 47 kilodaltons (TIP47), are cytoplasmic lipid droplet (LD)-associated proteins charac...

  15. Hormone signaling linked to silkmoth sex pheromone biosynthesis involves Ca2+/calmodulin-dependent protein kinase II-mediated phosphorylation of the insect PAT family protein Bombyx mori lipid storage droplet protein-1(BmLsd)

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The structurally-related members of the PAT family of proteins, which are so name based on similarity amongst perilipin, adipophilin/adipocyte differentiation-related protein (ADRP), and tail-interacting protein of 47 kilodaltons (TIP47), are cytoplasmic lipid droplet (LD)-associated proteins charac...

  16. Polymorphic amplified typing sequences (PATS) and pulsed-field gel electrophoresis (PFGE) yield comparable results in the strain typing of a diverse set of bovine Escherichia coli O157 isolates

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The PCR-based Escherichia coli O157 (O157) strain typing system, Polymorphic Amplified Typing Sequences (PATS), targets insertions-deletions (Indels) and single nucleotide polymorphisms (SNPs) at the XbaI and AvrII(BlnI) restriction enzyme sites, respectively, besides amplifying four known virulenc...

  17. Obstructive Sleep Apnea Using Watch-PAT 200 Is Independently Associated With an Increase in Morning Blood Pressure Surge in Never-Treated Hypertensive Patients.

    PubMed

    Cho, Jung Sun; Ihm, Sang-Hyun; Kim, Chan Joon; Park, Mahn-Won; Her, Sung-Ho; Park, Gyung-Min; Kim, Tae-Seok

    2015-09-01

    This study aimed to examine the association between obstructive sleep apnea (OSA) and morning blood pressure surge in never-treated patients with essential hypertension. This prospective study included a total of 58 patients (mean age, 51.7 years; 55.2% men) with never-treated essential hypertension. The patients were divided into non-OSA (n=23, 49.3±12.7 years) and OSA (n=35, 53.2±9.8 years) groups. The OSA group was defined as having an apnea-hypopnea index level >5 as measured by the Watch-PAT 200. The authors collected 24-hour ambulatory BP, plasma aldosterone concentration, and plasma renin activity data from all of the patients. The measured sleep-trough morning systolic blood pressure (SBP) increases were higher in the OSA group than in the non-OSA group (28.7±11.8 mm Hg vs 19.6±12.8 mm Hg, P=.008). The sleep-trough morning SBP increase was inversely correlated with the lowest oxygen saturation (r=-0.272, P=.039). OSA known to be associated with increased daytime and nocturnal sympathetic activity was associated with significantly higher sleep-trough morning SBP levels in this study.

  18. Quality by design (QbD), Process Analytical Technology (PAT), and design of experiment applied to the development of multifunctional sunscreens.

    PubMed

    Peres, Daniela D'Almeida; Ariede, Maira Bueno; Candido, Thalita Marcilio; de Almeida, Tania Santos; Lourenço, Felipe Rebello; Consiglieri, Vladi Olga; Kaneko, Telma Mary; Velasco, Maria Valéria Robles; Baby, André Rolim

    2017-02-01

    Multifunctional formulations are of great importance to ensure better skin protection from harm caused by ultraviolet radiation (UV). Despite the advantages of Quality by Design and Process Analytical Technology approaches to the development and optimization of new products, we found in the literature only a few studies concerning their applications in cosmetic product industry. Thus, in this research work, we applied the QbD and PAT approaches to the development of multifunctional sunscreens containing bemotrizinol, ethylhexyl triazone, and ferulic acid. In addition, UV transmittance method was applied to assess qualitative and quantitative critical quality attributes of sunscreens using chemometrics analyses. Linear discriminant analysis allowed classifying unknown formulations, which is useful for investigation of counterfeit and adulteration. Simultaneous quantification of ethylhexyl triazone, bemotrizinol, and ferulic acid presented at the formulations was performed using PLS regression. This design allowed us to verify the compounds in isolation and in combination and to prove that the antioxidant action of ferulic acid as well as the sunscreen actions, since the presence of this component increased 90% of antioxidant activity in vitro.

  19. Implementation of an artificial neural network as a PAT tool for the prediction of temperature distribution within a pharmaceutical fluidized bed granulator.

    PubMed

    Korteby, Yasmine; Mahdi, Yassine; Azizou, Amel; Daoud, Kamel; Regdon, Géza

    2016-06-10

    In this study, a novel in-line measurement technique of the air temperature distribution during a granulation process using a conical fluidized bed was designed and built for the purpose of measuring the temperature under the Process Analytical Technology (PAT) and introduced to predict the establishment of temperature profiles. Three sets of thermocouples were used, placed at different positions covering the whole operating range, connected to data acquisition measurement hardware, allowing an in-line acquisition and recording of temperatures every second. The measurements throughout the fluidized bed were performed in a steady state by spraying a solution of PVP onto a lactose monohydrate powder bed in order to make predictions of the temperature distribution and the hydrodynamics of the bed during the granulation process using Artificial Neural Networks (ANNs) and to establish the different temperature profiles for different process conditions through the precise predicted information by the constructed, trained, validated and tested neural network. The model's testing results showed a strong prediction capacity of the effects of process variables. Indeed, the predicted temperature values obtained with the ANN model were in good agreement with the values measured with in-line reference method and hence the method can have an application as a predictive control tool.

  20. A PAT approach to improve process understanding of high shear wet granulation through in-line particle measurement using FBRM C35.

    PubMed

    Huang, Jun; Kaul, Goldi; Utz, Julia; Hernandez, Pedro; Wong, Victor; Bradley, Daragh; Nagi, Arwinder; O'Grady, Des

    2010-07-01

    This article summarizes the investigation of in-line particle characterization during high shear wet granulation (HSWG) using focused beam reflectance measurement (FBRM) for enhanced process understanding, which is part of an effort to develop this drug product within the framework of quality by design (QbD) and process analytical technology (PAT). Traditionally, the effectiveness of in-line monitoring of HSWG processes is hindered by wet and sticky material fouling the probe resulting in inconsistent and erroneous data collection. For this study, a FBRM C35 probe was used which incorporates a scraping mechanism to maintain a clean probe window ensuring consistent measurements throughout each batch. The evaluations were conducted on nine scale-up DOE development batches and eight clinical sub-lots. In the DOE campaign, the purpose of FBRM was used to study the impact of varying water amount and wet massing time on granule dimension and count during granulation, while batch-to-batch variation or batch reproducibility was evaluated under the same process conditions for the clinical batches. In addition, a preliminary investigation of the most optimal probe position was conducted. The results indicate that FBRM is capable of monitoring the rate and degree of change to granule dimension/count during HSWG, and could be a potential technique for granulation endpoint determination.

  1. Quality-by-Design (QbD): an integrated process analytical technology (PAT) approach for real-time monitoring and mapping the state of a pharmaceutical coprecipitation process.

    PubMed

    Wu, Huiquan; Khan, Mansoor A

    2010-03-01

    In this work, an integrated PAT approach was developed for monitoring a pharmaceutical (naproxen) and a polymer (eudragit) coprecipitation process: real-time in-line near-infrared (NIR) absorbance monitoring, real-time on-line turbidity monitoring, and in situ crystal size monitoring. The data and information obtained through these three monitoring techniques confirmed the observation of the onsets of three distinct stages: incubation, nucleation, and crystal growth. The process trajectory constructed based on results of applying principal component analysis (PCA) to either process NIR spectra data or process turbidity profile, clearly demonstrated that various distinguishable process events, including incubation, nucleation, and crystal growth, could be accurately tracked and differentiated. These findings were further supported by process knowledge and information, such as process design, process sequence, thermodynamic and mass-transfer analysis. Therefore, this work provides a case study that illustrated a rational approach to develop a science-based and knowledge-based process monitoring strategy, which is essential for establishing both a suitable process control strategy and an operational process space for a pharmaceutical unit operation.

  2. Combining microwave resonance technology to multivariate data analysis as a novel PAT tool to improve process understanding in fluid bed granulation.

    PubMed

    Lourenço, Vera; Herdling, Thorsten; Reich, Gabriele; Menezes, José C; Lochmann, Dirk

    2011-08-01

    A set of 192 fluid bed granulation batches at industrial scale were in-line monitored using microwave resonance technology (MRT) to determine moisture, temperature and density of the granules. Multivariate data analysis techniques such as multiway partial least squares (PLS), multiway principal component analysis (PCA) and multivariate batch control charts were applied onto collected batch data sets. The combination of all these techniques, along with off-line particle size measurements, led to significantly increased process understanding. A seasonality effect could be put into evidence that impacted further processing through its influence on the final granule size. Moreover, it was demonstrated by means of a PLS that a relation between the particle size and the MRT measurements can be quantitatively defined, highlighting a potential ability of the MRT sensor to predict information about the final granule size. This study has contributed to improve a fluid bed granulation process, and the process knowledge obtained shows that the product quality can be built in process design, following Quality by Design (QbD) and Process Analytical Technology (PAT) principles.

  3. Both Sm-domain and C-terminal extension of Lsm1 are important for the RNA-binding activity of the Lsm1-7-Pat1 complex.

    PubMed

    Chowdhury, Ashis; Raju, Kalidindi K; Kalurupalle, Swathi; Tharun, Sundaresan

    2012-05-01

    Lsm proteins are a ubiquitous family of proteins characterized by the Sm-domain. They exist as hexa- or heptameric RNA-binding complexes and carry out RNA-related functions. The Sm-domain is thought to be sufficient for the RNA-binding activity of these proteins. The highly conserved eukaryotic Lsm1 through Lsm7 proteins are part of the cytoplasmic Lsm1-7-Pat1 complex, which is an activator of decapping in the conserved 5'-3' mRNA decay pathway. This complex also protects mRNA 3'-ends from trimming in vivo. Purified Lsm1-7-Pat1 complex is able to bind RNA in vitro and exhibits a unique binding preference for oligoadenylated RNA (over polyadenylated and unadenylated RNA). Lsm1 is a key subunit that determines the RNA-binding properties of this complex. The normal RNA-binding activity of this complex is crucial for mRNA decay and 3'-end protection in vivo and requires the intact Sm-domain of Lsm1. Here, we show that though necessary, the Sm-domain of Lsm1 is not sufficient for the normal RNA-binding ability of the Lsm1-7-Pat1 complex. Deletion of the C-terminal domain (CTD) of Lsm1 (while keeping the Sm-domain intact) impairs mRNA decay in vivo and results in Lsm1-7-Pat1 complexes that are severely impaired in RNA binding in vitro. Interestingly, the mRNA decay and 3'-end protection defects of such CTD-truncated lsm1 mutants could be suppressed in trans by overexpression of the CTD polypeptide. Thus, unlike most Sm-like proteins, Lsm1 uniquely requires both its Sm-domain and CTD for its normal RNA-binding function.

  4. Development of a Process Analytical Technology (PAT) for in-line monitoring of film thickness and mass of coating materials during a pan coating operation.

    PubMed

    Gendre, Claire; Genty, Muriel; Boiret, Mathieu; Julien, Marc; Meunier, Loïc; Lecoq, Olivier; Baron, Michel; Chaminade, Pierre; Péan, Jean Manuel

    2011-07-17

    The aim of this study was to perform in-line Near Infrared (NIR) measurements inside a pan coater to monitor a coating operation in real-time, by predicting the increases in mass of coating materials and coating thickness. A polymer combination of ethylcellulose/poly(vinyl-alcohol)-poly(ethylene-glycol) graft copolymer was used as functional aqueous coating. Coated tablets were sampled at regular intervals during the coating operation, then subjected to either simple and fast weighing (n=50) or accurate and non-destructive Terahertz Pulsed Imaging (TPI) measurements (n=3). Off-line NIR spectra analysis revealed that the coating operation could efficiently be controlled by focusing on two distinct NIR regions, related to absorption bands of ethylcellulose. Principal component analysis of in-line NIR spectra gave a clear classification of the collected coated tablets. Real-time quantitative monitoring of the coating operation was successfully performed from partial least square calibration models built using either TPI or weighing as reference method. Coating thicknesses as well as mass of coating materials used as primary values provided accurate NIR predictions. A comparison study demonstrated that both reference methods led to reliable and accurate real-time monitoring of the coating operation. This work demonstrated that in-line NIR measurements associated with multivariate analyses can be implemented to monitor in real-time a pan coating operation in order to fulfil the expectations of ICH Q8 guideline on pharmaceutical development, especially in terms of PAT control strategy and reduced end-product testing.

  5. The H+/K+-ATPase inhibitory activities of Trametenolic acid B from Trametes lactinea (Berk.) Pat, and its effects on gastric cancer cells.

    PubMed

    Zhang, Qiaoyin; Huang, Nianyu; Wang, Junzhi; Luo, Huajun; He, Haibo; Ding, Mingruo; Deng, Wei-Qiao; Zou, Kun

    2013-09-01

    Trametenolic acid B (TAB), the bioactive component in the Trametes lactinea (Berk.) Pat, was reported to possess cytotoxic activities and thrombin inhibiting effects. This study was performed to investigate the effects of TAB on H(+)/K(+)-ATPase and gastric cancer. The H(+)/K(+)-ATPase inhibitory activity was determined by gastric parietal cells. Compared to the normal control group, TAB (10, 20, 40 and 80 μg/mL) inhibited the H(+)/K(+)-ATPase activity by 15.97, 16.96, 24.86 and 16.25%, respectively. In the study, 36 Kunming mice were randomly divided into six groups: control, model, TAB-L (TAB, 5 mg/kg/day, i.g.), TAB-M (TAB, 20 mg/kg/day, i.g.), TAB-H (TAB, 40 mg/kg/day, i.g.) and omeprazole (OL, 10 mg/kg/day, i.g.). All mice except the control group were administrated with anhydrous alcohol (5.0 mL/kg, i.g.) for induced gastric-ulcer 1h after the 5th day. At the same time, the control mice were given the same volume of physiological saline. After 4h, TAB was evaluated for H(+)/K(+)-ATPase inhibitory activities of ulcerative gaster, gastric ulcer index and ulcer inhibition. In vitro, the anti-proliferation effect of TAB to gastric cancer cell (HGC-27) in acid environment was detected by MTT, and the apoptosis morphological changes were also observed by Hoechst 33258 dye assay. The results indicated that TAB inhibited moderately H(+)/K(+)-ATPase activity in vitro. Compared to the model group, TAB showed anti-ulcer effects in gastric tissue with the dosages of 20 and 5 mg/kg in vivo. Apart from that, TAB could selectively inhibit gastric cancer cell viability and reduce cell apoptosis against HGC-27 cells at low doses in acid environment.

  6. Mechanotransduction: getting morphogenesis down pat.

    PubMed

    Hardin, Jeff

    2011-05-10

    Embryonic morphogenesis requires the coordination of forces across multiple tissues and their associated extracellular matrices. A new study reports a mechanical feedback loop in the Caenorhabditis elegans embryo between muscle and epidermis that may provide a model for understanding how tissues coordinate morphogenetic events in the embryo.

  7. The world-class Howard's Pass SEDEX Zn-Pb district, Selwyn Basin, Yukon. Part I: trace element compositions of pyrite record input of hydrothermal, diagenetic, and metamorphic fluids to mineralization

    NASA Astrophysics Data System (ADS)

    Gadd, Michael G.; Layton-Matthews, Daniel; Peter, Jan M.; Paradis, Suzanne J.

    2016-03-01

    The Howard's Pass district, located in Yukon Territory, comprises 14 Zn-Pb sedimentary exhalative (SEDEX) deposits that collectively contain approximately 400.7 Mt grading at 4.5 % Zn and 1.5 % Pb. Sulfide mineralization is hosted in carbonaceous and calcareous to siliceous mudstones. Pyrite is a minor but ubiquitous component. Detailed petrographic analyses reveal that pyrite has a complex and protracted growth history, and multiple generations of pyrite are preserved in single grains. Combined electron probe microanalysis (EPMA) and laser ablation-inductively coupled mass plasma-mass spectrometry (LA-ICP-MS) of paragenetically complex pyrite reveal minor and trace element zonation that mimic textural features. These data provide information on the relative timing and cation content of depositional (i.e., ambient marine), hydrothermal, and metamorphic fluids. These data also identify a suite of nonore elements (Mn, As, Ag, Sb, and Tl) associated with the Zn-Pb mineralizing hydrothermal fluids. Lithogeochemical data and statistical results corroborate the microanalytical findings. These elements are associated with both syngenetic to earliest diagenetic pyrite and later diagenetic pyrite overgrowths, suggesting that SEDEX mineralization was not only the product of hydrothermal precipitates that settled on the seafloor, but also dense metalliferous brine also settled on, and percolated through, unconsolidated carbonaceous muds and precipitated metals. This genetic model is similar to that proposed for the Paleoproterozoic HYC Zn-Pb-Ag SEDEX deposit in northern Australia, and it is likely that common processes and ambient conditions led to the formation and preservation of both of these large SEDEX districts.

  8. Dale Long Emergency Medical Service Providers Protection Act

    THOMAS, 112th Congress

    Sen. Leahy, Patrick J. [D-VT

    2011-02-17

    02/17/2011 Read twice and referred to the Committee on the Judiciary. (text of measure as introduced: CR S885) (All Actions) Tracker: This bill has the status IntroducedHere are the steps for Status of Legislation:

  9. Drama in the Dale: Transformation through Community Drama

    ERIC Educational Resources Information Center

    Ward, Julie; Mills, Helen Frances; Anderson, Alan

    2013-01-01

    During the winter of 2011-2012, Weardale, England, was the setting for an ambitious informal adult education project. In this rural area in the northeast part of the country, the local arts collective, Jack Drum Arts, established a community play project entitled "The Bonny Moorhen." This dramatic undertaking aimed to retell the story of…

  10. Laboratory breeding of the European rabbit flea, Spilopsyllus cuniculi (Dale).

    PubMed Central

    Vaughan, J. A.; Coombs, M. E.

    1979-01-01

    A method is described for the laboratory breeding of the rabbit flea in which the immature stages are reared at constant temperature and humidity. Eggs are obtained by confining fleas taken from a rabbit and her nest shortly after parturition with two of her nestlings in an incubator for 24 h. The eggs are transferred to an artificial diet medium on which the immature stages are reared. On average a female flea produces 50 eggs during the first six days post-partum. At 25 degrees C, 95% of eggs hatched at 79% RH and 98% at 84% RH. Most eggs hatched on the third day after laying and hatching was completed by the fourth day. Significantly more fleas of both sexes were obtained when larvae were reared at 25 degrees C on a medium containing powdered 41B rodent diet than on one containing terrier meal. Both diets also contained yeast and dried rabbit blood. There was no significant difference between the numbers of fleas obtained at 79% RH and 84% RH. Significantly more fleas were also obtained when larvae were reared at 27 degrees C, 84% RH, than at 25 degrees C. Female fleas emerged sooner than males at both 27 degrees C and 25 degrees C. Fleas from the laboratory culture were heavier than those from wild nests. Female fleas were heavier than male fleas in both cases. PMID:512357

  11. Howard Parnes, MD | Division of Cancer Prevention

    Cancer.gov

    The Division of Cancer Prevention (DCP) conducts and supports research to determine a person's risk of cancer and to find ways to reduce the risk. This knowledge is critical to making progress against cancer because risk varies over the lifespan as genetic and epigenetic changes can transform healthy tissue into invasive cancer.

  12. Michael Howard: Military Historian and Strategic Analyst.

    DTIC Science & Technology

    1983-06-10

    he went to a major conference, Liddell Hart held court at the best available hotel ; whisky in one hand, pipe in the other, the one warm, the other...Admiral Buzzard, Mr. Healey, and Mr. Goold-Adams to draw up a list of seventy persons to invite to a conference to be held at the Bedford Hotel in

  13. Historicism, "Huckleberry Finn," and Howard Beach.

    ERIC Educational Resources Information Center

    Puttock, Kay

    1990-01-01

    Argues for the effectiveness of Mark Twain's "Huckleberry Finn" as a tool for increasing racial understanding. Suggests that class discussion of the book can make Black students feel as though they have valuable insights to contribute. Concludes that students' emotional reactions to Twain's novel demonstrate the continuing relevance of…

  14. The Federal Role in K-12 Mathematics Reform. Hearing before the Subcommittee on Early Childhood, Youth and Families of the Committee on Education and the Workforce, House of Representatives, One Hundred Sixth Congress, Second Session (February 2, 2000).

    ERIC Educational Resources Information Center

    Congress of the U.S., Washington, DC. House Committee on Education and the Workforce.

    This hearing focuses on the federal role in K-12 mathematics reform. Prepared statements are provided from: Michael N. Castle, Chairman, Subcommittee on Early Childhood Youth, and Families; Dale E. Kildee, Ranking Member, Subcommittee on Early Childhood Youth, and Families; Howard P. "Buck" Mckeon, Chairman, Subcommittee on Postsecondary…

  15. Advancing Humanities Studies at Community, Technical, and Junior Colleges.

    ERIC Educational Resources Information Center

    Eisenberg, Diane U.; And Others

    The American Association of Community and Junior Colleges' (AACJC's) two-year Advancing the Humanities Project (AHP) has assisted selected community colleges in promoting the humanities on their campuses. Parts I and II of this report on the AHP present statements by Dale Parnell and Judith Jeffrey Howard about the AACJC's humanities initiatives…

  16. America's Teacher Colleges: Are They Making the Grade? Hearing before the Subcommittee on 21st Century Competitiveness of the Committee on Education and the Workforce. House of Representatives, One Hundred Eighth Congress, First Session (May 20, 2003).

    ERIC Educational Resources Information Center

    Congress of the U.S., Washington, DC. House Committee on Education and the Workforce.

    A hearing was held to explore whether U. S. teachers' colleges are doing an adequate job. Opening statements by Representative Howard P. McKeon and Representative Dale Kildee set the stage for the testimony of these witnesses: (1) Lisa Graham Keegan, Chief Executive Officer, Education Leaders Council; (2) Kati Haycock, Director, The Education…

  17. Improving Adult Education for the 21st Century. Hearing Before the Subcommittee on 21st Century Competitiveness of the Committee on Education and the Workforce, House of Representatives, One Hundred Eighth Congress, First Session. Hearing held in Washington, DC, March 4, 2003.

    ERIC Educational Resources Information Center

    Congress of the U.S., Washington, DC. House Committee on Education and the Workforce.

    A hearing was held to discuss the prior four years of implementation of the Adult Education and Family Literacy Act, and to recommend further improvements. The opening statements of Chairman Howard McKeon and Dale E. Kildee introduce the meeting and discuss the importance of promoting an educated populace that will maintain the United States'…

  18. Simultaneous Enrichment of Cysteine-containing Peptides and Phosphopeptides Using a Cysteine-specific Phosphonate Adaptable Tag (CysPAT) in Combination with titanium dioxide (TiO2) Chromatography*

    PubMed Central

    Huang, Honggang; Haar Petersen, Martin; Ibañez-Vea, Maria; Lassen, Pernille S.; Larsen, Martin R.; Palmisano, Giuseppe

    2016-01-01

    Cysteine is a rare and conserved amino acid involved in most cellular functions. The thiol group of cysteine can be subjected to diverse oxidative modifications that regulate many physio-pathological states. In the present work, a Cysteine-specific Phosphonate Adaptable Tag (CysPAT) was synthesized to selectively label cysteine-containing peptides (Cys peptides) followed by their enrichment with titanium dioxide (TiO2) and subsequent mass spectrometric analysis. The CysPAT strategy was developed using a synthetic peptide, a standard protein and subsequently the strategy was applied to protein lysates from Hela cells, achieving high specificity and enrichment efficiency. In particular, for Cys proteome analysis, the method led to the identification of 7509 unique Cys peptides from 500 μg of HeLa cell lysate starting material. Furthermore, the method was developed to simultaneously enrich Cys peptides and phosphorylated peptides. This strategy was applied to SILAC labeled Hela cells subjected to 5 min epidermal growth factor (EGF) stimulation. In total, 10440 unique reversibly modified Cys peptides (3855 proteins) and 7339 unique phosphopeptides (2234 proteins) were simultaneously identified from 250 μg starting material. Significant regulation was observed in both phosphorylation and reversible Cys modification of proteins involved in EGFR signaling. Our data indicates that EGF stimulation can activate the well-known phosphorylation of EGFR and downstream signaling molecules, such as mitogen-activated protein kinases (MAPK1 and MAPK3), however, it also leads to substantial modulation of reversible cysteine modifications in numerous proteins. Several protein tyrosine phosphatases (PTPs) showed a reduction of the catalytic Cys site in the conserved putative phosphatase HC(X)5R motif indicating an activation and subsequent de-phosphorylation of proteins involved in the EGF signaling pathway. Overall, the CysPAT strategy is a straight forward, easy and promising

  19. "The Day All of the Different Parts of Me Can Come Along": Intersectionality and U.S. Third World Feminism in the Poetry of Pat Parker and Willyce Kim.

    PubMed

    Van Ausdall, Mimi Iimuro

    2015-01-01

    This article brings to light the poetry of Pat Parker and Willyce Kim, two key figures within the 1970s and '80s women in print movement. While Parker and Kim have been rightly placed within African-American and Asian-American histories, respectively, and working-class and lesbian-feminist literary histories, their work is most fully understood within the context of U.S. Third World Feminism. Through close readings of poetic form and content in addition to engagement with current debates about intersectionality as a methodology, the article links Kim and Parker's works to central contributions of U.S. Third World Feminism such as intersectionality and power across and within difference that continue to influence feminist theory today.

  20. A PDDA/poly(2,6-pyridinedicarboxylic acid)-CNTs composite film DNA electrochemical sensor and its application for the detection of specific sequences related to PAT gene and NOS gene.

    PubMed

    Yang, Tao; Zhang, Wei; Du, Meng; Jiao, Kui

    2008-05-30

    2,6-Pyridinedicarboxylic acid (PDC) was electropolymerized on the glassy carbon electrode (GCE) surface combined with carboxylic group-functionalized single-walled carbon nanotubes (SWNTs) by cyclic voltammetry (CV) to form PDC-SWNTs composite film, which was rich in negatively charged carboxylic group. Then, poly(diallyldimethyl ammonium chloride) (PDDA), a linear cationic polyelectrolyte, was electrostatically adsorbed on the PDC-SWNTs/GCE surface. DNA probes with negatively charged phosphate group at the 5' end were immobilized on the PDDA/PDC-SWNTs/GCE due to the strong electrostatic attraction between PDDA and phosphate group of DNA. It has been found that modification of the electrode with PDC-SWNTs film has enhanced the effective electrode surface area and electron-transfer ability, in addition to providing negatively charged groups for the electrostatic assembly of cationic polyelectrolyte. PDDA plays a key role in the attachment of DNA probes to the PDC-SWNTs composite film and acts as a bridge to connect DNA with PDC-SWNTs film. The cathodic peak current of methylene blue (MB), an electroactive label, decreased obviously after the hybridization of DNA probe (ssDNA) with the complementary DNA (cDNA). This peak current change was used to monitor the recognition of the specific sequences related to PAT gene in the transgenic corn and the polymerase chain reaction (PCR) amplification of NOS gene from the sample of transgenic soybean with satisfactory results. Under optimal conditions, the dynamic detection range of the sensor to PAT gene target sequence was from 1.0x10(-11) to 1.0x10(-6) mol/L with the detection limit of 2.6x10(-12) mol/L.

  1. Anticancer activity of fungal taxol derived from Botryodiplodia theobromae Pat., an endophytic fungus, against 7, 12 dimethyl benz(a)anthracene (DMBA)-induced mammary gland carcinogenesis in Sprague Dawley rats.

    PubMed

    Pandi, M; Manikandan, R; Muthumary, J

    2010-01-01

    Breast cancer is the second most prevalent cancer worldwide and their incidence increases gradually. Taxol (paclitaxel), a potent anticancer drug, is naturally isolated from the bark of the Pacific yew. Taxol is widely used in the treatment of ovarian, lung and breast cancer. The increased demand for taxol, coupled with its limited availability from the protected Pacific yew, has had researchers scrambling for alternate sources. The purpose of the present study is to investigate chemopreventive effect of fungal taxol derived from a novel endophytic fungus Botryodiplodia theobromae Pat., isolated from a medicinal plant Morinda citrifolia Linn. The fungal taxol is found to be active against the 7, 12 dimethyl benz(a)anthracene (DMBA)-induced mammary gland carcinogenesis in Sprague dawley rats. The enzymic and non-enzymic antioxidants i.e. superoxide dismutase (SOD), catalase (CAT), glutatione peroxidase (GPx), glutatione-S-transferase (GST), reduced glutathione (GSH), vitamin C and vitamin E were evaluated in control and experimental groups. Lipid peroxides levels (LPO) were also tested. Histological analysis of breast tissue was analyzed by haematoxylin and eosin staining to assess the cytoprotective role of fungal taxol active against breast cancer. Immunohistochemical analyses were also performed to evaluate the effect of fungal taxol on the inflammatory marker such as Cyclooxygenase-2 (COX-2) in control and experimental groups. The results showed that the fungal taxol significantly suppresses the DMBA-induced breast cancer in Sprague dawley rats.

  2. Managing the Arctic Thaw: A Joint Interagency Approach to a Potential Hot Spot

    DTIC Science & Technology

    2011-05-04

    2011). Dale, Catherine, Nina Serafino, and Pat Towell . Organizing the U.S. Government for National Security: Overview of the Interagency Reform...34 Defense Concepts 4, no. 4 (2010): 22-31. Macalister, Terry . "Climate change could lead to Arctic conflict, warns senior NATO Commander." Guardian.co.uk

  3. Field Turf System Has Irrigation Down PAT

    ERIC Educational Resources Information Center

    Day, C. William

    1973-01-01

    Explains the process whereby Goshen High School (Indiana) acquired a football field that is never muddy, but which is never covered with expensive sheeting; and that has green grass the year around, but which no one ever sprinkles. It also offers firmness for running, resiliency for falling, traction for turning, and a flat, highly uniform field.…

  4. Energy Consumption of Battery-Operated Screwdriver at Assembling Fixed Threaded Joints / Akumulatora Skrūvgrieža Elektroenerģijas Patēriņa Aprēķins, Veicot Nekustīga Vītņu Savienojuma Salikšanu Atkarībā No Operatora Reakcijas Laika

    NASA Astrophysics Data System (ADS)

    Grinevich, I.; Mozga, N.; Rudzitis, J.

    2013-10-01

    The electrical energy consumption of battery-operated screwdrivers, which are widely used in many industries, e.g. automotive, heavy, chemical, etc., can be considerably reduced. This would allow increasing the service life of a battery and reducing the time of its charging, thus increasing the productivity and decreasing the prime cost of this battery. It is shown that up to 17.9% of electrical energy could be saved at assembling fixed threaded joints by removing power from the screwdriver immediately after reaching the required tightening torque. Dotajā rakstā uzmanība tiek pievērsta akumulatora skrūvgrieža elektroenerģijas patēriņa samazināšanas iespējām sakarā ar problēmas aktualitāti šīs grupas plaši pielietotajiem instrumentiem. Elektroenerģijas patēriņa samazināšana masveida ražošanā, kur tiek izmantoti akumulatora skrūvgrieži, ļautu paildzināt akumulatora baterijas kalpošanas mūžu un samazināt uzlādēšanas laiku, kas, savukārt, novestu pie ražības palielināšanas un produkcijas pašizmaksas samazināšanas.

  5. Howard University Flow Cytometric Sorter For Research and Education

    DTIC Science & Technology

    2015-08-04

    reformation (Dazhi Yang), microbial ecologies (Patrick Ymele-Leki), cell cycle and cancer onset (Sharma Sudha), immunotherapies and antigen presentation...instrument). Current researchers using the instrument have sciences that include bone reformation (Dazhi Yang), microbial ecologies (Patrick Ymele-Leki

  6. Seeking the Roots of Talent: An Interview with Howard Gardner.

    ERIC Educational Resources Information Center

    Buescher, Thomas M.

    1985-01-01

    An interview with H. Gardner, a research scientist in neuropsychology, examines his work on the emergence of symbols in children and the implications of early talent development for gifted and creative children. He discusses his research on the multiple talents comprising his conception of intelligence. (CL)

  7. The ethical mind. A conversation with psychologist Howard Gardner.

    PubMed

    Gardner, Howard

    2007-03-01

    Business leadership has become synonymous in the public eye with unethical behavior. Widespread scandals, massive layoffs, and inflated executive pay packages have led many to believe that corporate wrongdoing is the status quo. That's why it's more important than ever that those at the top mend relationships with customers, employees, and other stakeholders. Professor Gardner has spent many years studying the relationship between psychology and ethics at Harvard's Graduate School of Education. In this interview with HBR senior editor Bronwyn Fryer, Gardner talks about what he calls the ethical mind, which helps individuals aspire to do good work that matters to their colleagues, companies, and society in general. In an era when workers are overwhelmed by too much information and feel pressured to win at all costs, Gardner believes, it's easy to lose one's way. What's more, employees look to leaders for cues as to what's appropriate and what's not. So if you're a leader, what's the best way to stand up to ethical pressures and set a good example? First and foremost, says Gardner, you must believe that retaining an ethical compass is essential to the health of your organization. Then you must state your ethical beliefs and stick to them. You should also test yourself rigorously to make sure you're adhering to your values, take time to reflect on your beliefs, find multiple mentors who aren't afraid to speak truth to your power, and confront others' egregious behavior as soon as it arises. In the end, Gardner believes, the world hangs in the balance between right and wrong, good and bad, success and disaster. "You need to decide which side you're on:" he concludes, "and do the right thing."

  8. [Activities of the Department of Electrical Engineering, Howard University

    NASA Technical Reports Server (NTRS)

    Yalamanchili, Raj C.

    1997-01-01

    Theoretical derivations, computer analysis and test data are provided to demonstrate that the cavity model is a feasible one to analyze thin-substrate, rectangular-patch microstrip antennas. Seven separate antennas were tested. Most of the antennas were designed to resonate at L-band frequencies (1-2 GHz). One antenna was designed to resonate at an S-band (2-4 GHz) frequency of 2.025 GHz. All dielectric substrates were made of Duroid, and were of varying thicknesses and relative dielectric constant values. Theoretical derivations to calculate radiated free space electromagnetic fields and antenna input impedance were performed. MATHEMATICA 2.2 software was used to generate Smith Chart input impedance plots, normalized relative power radiation plots and to perform other numerical manipulations. Network Analyzer tests were used to verify the data from the computer programming (such as input impedance and VSWR). Finally, tests were performed in an anechoic chamber to measure receive-mode polar power patterns in the E and H planes. Agreement between computer analysis and test data is presented. The antenna with the thickest substrate (e(sub r) = 2.33,62 mils thick) showed the worst match to theoretical impedance data. This is anticipated due to the fact that the cavity model generally loses accuracy when the dielectric substrate thickness exceeds 5% of the antenna's free space wavelength. A method of reducing computer execution time for impedance calculations is also presented.

  9. Howard J. Burnett Center, Washington & Jefferson College, Washington, Pennsylvania.

    ERIC Educational Resources Information Center

    Design Cost Data, 2003

    2003-01-01

    Describes the title building, including educational context and design goals. Includes a general description; information on the architect, construction team, and manufacturers and suppliers; and a case study of costs and specifications. (EV)

  10. NASA/Howard University Large Space Structures Institute

    NASA Technical Reports Server (NTRS)

    Broome, T. H., Jr.

    1984-01-01

    Basic research on the engineering behavior of large space structures is presented. Methods of structural analysis, control, and optimization of large flexible systems are examined. Topics of investigation include the Load Correction Method (LCM) modeling technique, stabilization of flexible bodies by feedback control, mathematical refinement of analysis equations, optimization of the design of structural components, deployment dynamics, and the use of microprocessors in attitude and shape control of large space structures. Information on key personnel, budgeting, support plans and conferences is included.

  11. Howard Coble Coast Guard and Maritime Transportation Act of 2014

    THOMAS, 113th Congress

    Rep. Hunter, Duncan D. [R-CA-50

    2014-12-01

    12/04/2014 Received in the Senate and Read twice and referred to the Committee on Commerce, Science, and Transportation. (All Actions) Notes: For further action, see S.2444, which became Public Law 113-281 on 12/18/2014. Tracker: This bill has the status Passed HouseHere are the steps for Status of Legislation:

  12. Evaluation of Water Quality--Howard AFB, Panama Bacteriological Quality

    DTIC Science & Technology

    1991-04-01

    reduced) by surfaces, age of the biofilm, encapsulation, and nutrient effects. Disinfec- tion by monochloramine was only effected by surfaces." (8...to monochloramine disinfection (9). A number of major water systems in the US have found combined chlorine/chloramines an effective method of...for 2 weeks did not show significant changes in viability, but if treated with 4 mg/l of monochloramine for 2 weeks, the biofilms exhibited a 3-log

  13. Roswell Park Cancer Institute / Howard University Prostate Cancer Scholars Program

    DTIC Science & Technology

    2015-10-01

    activities to familiarize and inform them of program expectations, deadlines and RPCI policies. Students received training in responsible conduct of...peers from undergraduate research programs hosted at academic and research centers in the Buffalo area. Students participated in organized social ...Approved OMB No. 0704-0188 Public reporting burden for this collection of information is estimated to average 1 hour per response , including the time for

  14. Conversations with Four Highly Productive Educational Psychologists: Patricia Alexander, Richard Mayer, Dale Schunk, and Barry Zimmerman

    ERIC Educational Resources Information Center

    Patterson-Hazley, Melissa; Kiewra, Kenneth A.

    2013-01-01

    This article seeks to answer the questions: Who are the most productive and influential educational psychologists? What factors characterize these educational psychologists? And, what advice might they pass along to budding scholars? To determine the top educational psychologists, we surveyed the membership of Division 15 (Educational Psychology)…

  15. Dale Avenue School Early Childhood Education Center Project. Research Bulletin Volume III, No. 2, June 1973.

    ERIC Educational Resources Information Center

    Paterson Board of Education, NJ.

    Reported are results of an evaluation of the handwriting skills of first, second, and third level students who were part of an urban early childhood education project for culturally disadvantaged children in Paterson, New Jersey. Provided is a summary of A. Gesell and F. Ilg's recommendations for handwriting instruction for kindergarten through…

  16. Carbonate chemistry of surface waters in a temperate karst region: the southern Yorkshire Dales, UK

    NASA Astrophysics Data System (ADS)

    Pentecost, Allan

    1992-11-01

    A detailed study of surface water chemistry is described from an important limestone region in northern England. Major ions and pH were determined for 485 sites (springs, seeps, streams, rivers and lakes) during summertime. The saturation state of the waters with respect to calcite was determined as the calcite saturation ratio (Ω). An unexpectedly large number of samples were found to be supersaturated (65.5% of the 268 km of watercourses surveyed). As a consequence, several streams entering major cave systems were incapable of further limestone solution, at least during periods of low flow. Many waters were supersaturated from their source and some deposited travertine. A significant negative correlation was found between spring discharge and both (Ω) and pH. Supersaturation was caused primarily by atmospheric degassing, with some contribution from aquatic plant photosynthesis. The median total dissolved inorganic carbon and Ca concentrations were 2.49 and 1.35 millimoles 1 -1 respectively. Calcium originated exclusively from limestone, and carbon dioxide mainly from the soil and dissolved limestone. South facing catchments provided springwaters with significantly higher levels of TDIC and Ca when compared with north facing catchments. The study suggests that acid rain made a measurable contribution to limestone dissolution. Carboniferous limestone denudation rates were estimated as 54 to 63 m 3 km -2 a -1 (54 to 63 mm 1000 years -1). About 50% of the Mg came from limestone and the remainder, together with most K, Na, SO 4 and Cl from precipitation. Concentrations of dissolved nutrients were low, medians for NO 3, NH 4, total PO 4 and SiO 3 were 24 μmol, 1.4 μmol, 0.64 μmol and 15.5 μmol 1 -1 respectively. The concentration of a further 23 trace elements was determined.

  17. T. Dale Stewart's perspective on his career as a forensic anthropologist at the Smithsonian.

    PubMed

    Ubelaker, D H

    2000-03-01

    T. D. Stewart (1901-1997) is internationally recognized as an early leader in forensic anthropology. In a series of taped interviews in 1975 and in 1986, Stewart discusses his professional development. The interviews document his early education in Delta, Pennsylvania, his long career at the Smithsonian Institution in Washington, DC and aspects of his many contributions to forensic anthropology. His well-known careful editorial work and exhaustive, problem-oriented research approach may have been influenced by his early training in his hometown bank and his many years of apprenticeship under Ales Hrdlicka (1869-1943). Stewart describes aspects of the difficulties of working for Hrdlicka, yet credits the work ethic established by him as a formative factor in his own prodigious productivity.

  18. Health-Hazard Evaluation Report HETA 85-195-1768, Dale Electronics, Incorporated, Norfolk, Nebraska

    SciTech Connect

    Lee, S.A.

    1987-01-01

    An evaluation was made of exposure to butyl cellosolve, epichlorohydrin, nickel, chromium, antimony, and total particulates for workers in an electronic resistor manufacturing process. No exposure to butyl cellosolve was detected among liquid coaters and no epichlorohydrin was detected in the process area air samples. No exposure to nickel or chromium was detected among mechanical-grinding spirallers. One laser spiraller was exposed to 8 micrograms/m/sup 3/ of nickel and 8 microg/m/sup 3/ chromium. Trace amounts of nickel were noted in the breathing zones of one plating lab worker and one calibrator. Total particulate exposures ranged from 0.1 to 0.9 mg/m/sup 3/. The author concludes that there were no known overexposures to contaminants at this facility and recommends further checking.

  19. "13 Angry Men": Dale Bumper's "Ad Hominem" Impeachment Trial of President Clinton.

    ERIC Educational Resources Information Center

    Brovero, Adrienne F.

    2000-01-01

    Analyzes Senator Bumpers' argument in the Clinton impeachment proceedings as a bias type of "ad hominem" argument. Notes specifically Bumpers' argument that the House Managers were not fulfilling their proper roles in the proceedings. Claims Bumpers' bias position was reasonable in the argumentative context in which it took place. (NH)

  20. Fonction de Green pour les matériaux à anisotropie ellipsoïdale

    NASA Astrophysics Data System (ADS)

    Pouya, Ahmad

    2007-07-01

    An explicit solution for the Green function of the infinite medium in linear elastic materials is known only for isotropic and transversely isotropic materials and for some other cases obtained by a linear transformation. In this Note, we consider a family of linear elastic materials characterized by the property that for some elastic parameters, the polar diagram giving the value of these parameters in different directions is ellipsoidal. We show first that this family of materials depends on 12 independent parameters and that the elastic moduli tensor of these materials can be defined by two second order symmetric tensors. We show then that for these materials an explicit solution for the Green function of the infinite medium can be given and we give a first expression for this solution. To cite this article: A. Pouya, C. R. Mecanique 335 (2007).

  1. Blood and Gore, Fens and Fears: "Beowulf" Battles the Dale-Chall Monster.

    ERIC Educational Resources Information Center

    Wagner, Betty Jane

    Unfortunately, textbooks of the elementary and junior high school levels receive very little of the honest, critical evaluation which is given trade books in children's literature. One vivid example of this fact was seen in the recent arduous but successful attempt to include "Beowul" in a fourth-grade literature text which was being…

  2. STS-8 onboard crew press conference

    NASA Technical Reports Server (NTRS)

    1983-01-01

    Six news reporters listen to a response from Astronaut Guion S. Bluford (note TV monitor) in a rare space-to-Earth press conference involving all the STS-8 crew. The participants are, left to right, Gary Schwitzer, Cable News Network; Morton Dean, CBS; Roy Neal, NBC; Lynn Sherr, ABC; Howard Benedict, Associated Press; Al Rossiter, United Press International. The astronauts on the monitor are Richard H. Truly, cneter left, crew commander; Daniel C. Brandenstein, lower left, pilot; and Dr. William E. Thornton, upper left, Guion S. Bluford, upper right; and Dale E. Gardner, all mission specialists.

  3. The postmortem fate of Pat Gregory: a disinterred Native American.

    PubMed

    Murad, T A; Murad, T D

    2000-03-01

    In late June 1990, the Mono County Sheriffs Department in Bridgeport, CA contacted the Physical Anthropology Human Identification Laboratory (PAHIL) at California State University, Chico to seek assistance in the identification of a recently discovered skull. To assist with possible identification, the cranium received a classic physical anthropological/morphological analysis to suggest the decedent's sex, age at death, ancestral affiliation, and uniqueness. It was concluded the cranium was that of an older male, and someone with mixed ancestry, probably Native American/White. Suggested uniquenesses were an eroded and greasy texture, with adhering white sand, evidence of healed antemortem nasal fractures, and a bifid left occipital condyle. The cranium was confiscated from a man suspected of vandalizing a Native American cemetery just south of the community of Lee Vining. The cemetery was established in the mid-1800's by local Native American tribes. Although ownership of the land was disputed by the US Forest Service (the Inyo National Forest), and the Los Angeles Department of Water and Power (LADWP), county authorities claimed that because the incident involved the desecration of a cemetery and human remains, it was a legal issue, and therefore, the Sheriff's Department had jurisdiction over the case if not the land. The suspect pled guilty to the possession of Native American remains but claimed not to have desecrated a grave. Over the next year and a half, members of the Native American community representing various tribes sought the return of the cranium, while also seeking assurance that it belonged to the vandalized grave. While county, US Forest Service, and LADWP officials continued to argue over whom had jurisdiction of the remains the superior court judge ordered the county to pay for any analysis necessary to determine if the cranium belonged to the decedent in question. This report addresses the conclusions of that analysis and the disposition of the case. Furthermore, the report addresses the forensic value to Native Americans of the continued study of a wide variety of human skeletal remains.

  4. It's All about the Money: Chris and Pat Compare Salaries

    ERIC Educational Resources Information Center

    Renard, Monika K.

    2008-01-01

    Can you name 20 influences on pay that could cause a difference in earnings between two ostensibly equal employees? This short, involving exercise can be used to illustrate the numerous influences that affect how employees' pay is determined, for example, education, experience required for the job, supply and demand, company size, seniority, and…

  5. Flood Mitigation Planning Using HEC-SAM.

    DTIC Science & Technology

    1980-06-01

    the HEC who contributed substantially include R. Pat Webb--responsible for most of the developmental work and day-to-day servicing, Shelle Barkin --data...23 pages. #48 Direct Runoff Hydroqraph Parameters Versus Urbanization, David L. Gundlach, September 1976, 10 pages. #49 Experience of HEC in...Sedimentation, David T. Williams, October 1977, 39 pages. #51 Design of Flood Control Improvements by Systems Analysis: A Case Study, Howard 0. Reese, Arnold

  6. Compliance Monitoring of Juvenile Yearling Chinook Salmon and Steelhead Survival and Passage at The Dales Dam, Spring 2011

    SciTech Connect

    Skalski, John R.; Townsend, Richard L.; Seaburg, Adam; Johnson, Gary E.; Ploskey, Gene R.; Carlson, Thomas J.

    2012-02-01

    The study estimated dam passage survival at The Dalles Dam as stipulated by the 2008 Federal Columbia River Power System (FCRPS) Biological Opinion (BiOp) and provided additional performance measures as stipulated in the Columbia Basin Fish Accords. This summary report focuses on spring run stocks, yearling Chinook salmon and steelhead.

  7. Petition to Object to the Eastern Kentucky Power Coop. William C. Dale Power Station, Ford, Kentucky, Title V Operating Permit

    EPA Pesticide Factsheets

    This document may be of assistance in applying the Title V air operating permit regulations. This document is part of the Title V Petition Database available at www2.epa.gov/title-v-operating-permits/title-v-petition-database. Some documents in the database are a scanned or retyped version of a paper photocopy of the original. Although we have taken considerable effort to quality assure the documents, some may contain typographical errors. Contact the office that issued the document if you need a copy of the original.

  8. Particle size and concentration adjustments of tomato products for Howard mold count.

    PubMed

    Bandler, R; Cichowicz, S M; Cichowicz, S; Floyd, D; Kaminski, J; Russell, G; Senff, W; Trauba, R

    1981-05-01

    The present AOAC method for mold counts of tomato products (44.096) provides no inter-product standardization of concentration among juice, sauce, paste, puree, and catsup; no intra-product standardization of concentration for juice, sauce, and catsup; and no adjustment for degree of comminution. Use of the official method, therefore, could result in an artificially increased mold count for products which have undergone extreme comminution. A new method was developed to adjust all products to the same concentration and grind them to a uniform particle size, thereby ensuring comparable mold counts on products produced by different processes. Collaborative study results showed equal repeatability for both the official and the proposed methods and a lower coefficient of variation for the proposed method.

  9. Grubb, Thomas (1800-78) and Grubb, Howard (1844-1931)

    NASA Astrophysics Data System (ADS)

    Murdin, P.

    2000-11-01

    Irish telescope makers, based in Dublin, then in St Albans, England. Thomas was born in Waterford and set up a machinery business. After making a 9 in telescope for himself, he made many giant reflecting telescopes commercially. One of his first contracts was for a Cassegrain reflector for ROMNEY ROBINSON of Armagh Observatory, which introduced mechanical design features of significance for the f...

  10. The First Seven...and the Eighth: A Conversation with Howard Gardner.

    ERIC Educational Resources Information Center

    Checkley, Kathy

    1997-01-01

    Reviews seven multiple-intelligence forms (linguistic, logical-mathematical, spatial, bodily kinesthetic, musical, interpersonal, intrapersonal) and adds naturalist intelligence, the ability to discriminate among living things. Considers a ninth form (existential intelligence), challenges the IQ concept and common testing practices, and urges…

  11. Bertolt Brecht and Roger Howard: Revolutionary Theatre and Socio-Political Worries

    ERIC Educational Resources Information Center

    Shalghin, Akram

    2014-01-01

    This paper deals with the question of revolutionary literary texts. It examines the painful, or even brutal, measures that could be taken at times when the revolutionary figures depicted in the literary texts are themselves subject to control by a party line. While this paper shows that the noble intentions would not suffice for conducting…

  12. 75 FR 43138 - Ochoco National Forest, Lookout Mountain Ranger District; Oregon; Howard Elliot Johnson Fuels and...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-07-23

    ... Environmental Protection Agency (EPA) and available for public review by December, 2010. The EPA will publish a... prepare an environmental impact statement. SUMMARY: The Ochoco National Forest is preparing an environmental impact statement (EIS) to analyze the effects of managing fuels and vegetation within the...

  13. Accreditation. Chassin and Joint Commission aim to inspire. Interview by Howard Larkin.

    PubMed

    Chassin, Mark; Conway, James B; Umbdenstock, Richard J; Dwyer, James; Langberg, Michael L; Petasnick, William D

    2010-03-01

    After two years at the helm of the Joint Commission, Mark Chassin, M.D., is pressing forward with efforts to turn the Joint Commission into a partner with hospitals and other providers and transform health care into a "high reliability" enterprise along the lines of the aviation and nuclear energy industries. Hospitals & Health Networks asked health care experts and Chassin himself how he's doing so far.

  14. Prioritized Reliability-Risk$ Optimization for a Hydro-Thermal System CddHoward Consulting Ltd

    NASA Astrophysics Data System (ADS)

    Howard, C. D.; Howard, J. C.

    2010-12-01

    This paper provides a real-world example of a hydro-economic model for risk estimation and impact assessment. Ensembles of forecasted reservoir inflows were used to drive an integrated hydro-thermal two-reservoir stochastic long term economic optimization model. The optimization objective was to minimize long term thermal energy purchases by recommending the best current operating decisions within policies on long-term Risk (probabilistic cost). Risk was determined as a function of Reliability to meet forecasted energy load. Reservoir End of Month Rule Curves Reliability: Thermal Risk and Hydro Revenue

  15. 75 FR 42814 - Central Railroad Company of Indianapolis-Abandonment Exemption-in Howard County, IN

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-07-22

    ... user of rail service on the line (or by a state or local government entity acting on behalf of such user) regarding cessation of service over the line either is pending ] with the Surface Transportation... expression of intent to file an offer of financial assistance (OFA) has been received, this exemption will...

  16. Howard Gardner, Meet Benjamin Bloom: Strategies for the Future Enliven Methods from the Past.

    ERIC Educational Resources Information Center

    Savitz, Fred

    Classic theories in pedagogy such as those of John Dewey and Jean Piaget establish the foundation upon which preservice students in a social studies methods course build new knowledge about the teaching/learning paradigm. The constructivist philosophy emanating from these theories provides the rationale for a technique designed to enable students…

  17. Howard P. "Buck" McKeon National Defense Authorization Act for Fiscal Year 2015

    THOMAS, 113th Congress

    Rep. McKeon, Howard P. "Buck" [R-CA-25

    2014-04-09

    06/05/2014 Received in the Senate. Read twice. Placed on Senate Legislative Calendar under General Orders. Calendar No. 425. (All Actions) Notes: For further action, see H.R.3979, which became Public Law 113-291 on 12/19/2014. Tracker: This bill has the status Passed HouseHere are the steps for Status of Legislation:

  18. Antimicrobial activity and phytochemical screening of Buchenavia tetraphylla (Aubl.) R. A. Howard (Combretaceae: Combretoideae).

    PubMed

    de Oliveira, Ygor Lucena Cabral; Nascimento da Silva, Luís Cláudio; da Silva, Alexandre Gomes; Macedo, Alexandre José; de Araújo, Janete Magali; Correia, Maria Tereza dos Santos; da Silva, Márcia Vanusa

    2012-01-01

    This study evaluated the antimicrobial and hemolytic activities and phytochemical constituents of hydroalcoholic extract and its fractions from Buchenavia tetraphylla leaves. Cyclohexane (BTCF), ethyl acetate (BTEF), and n-butanol-soluble (BTSBF) and non-soluble (BTNBF) fractions were obtained from a liquid-liquid partition of hydroalcoholic extract (BTHE) from B. tetraphylla leaves. The hemolytic activity of active fractions was checked. The BTHE inhibited the growth of Micrococcus luteus (MIC: 0.10 mg/mL), Pseudomonas aeruginosa (MIC: 0.20 mg/mL), Mycobacterium smegmatis (MIC: 0.39 mg/mL), Proteus vulgaris, and Staphylococcus aureus (MIC: 0.78 mg/mL for both). The more active fractions were BTCF and BTBSF. BTCF showed better potential to inhibit M. luteus (0.10 mg/mL), P. aeruginosa (0.20 mg/mL), S. enteritidis (0.39 mg/mL), and S. aureus (1.56 mg/mL). BTBSF showed the best results for M. luteus (0.10 mg/mL), M. smegmatis, B. subtilis (0.39 mg/mL for both), and P. vulgaris (0.10 mg/mL). The HC50 were greater than observed MIC: 20.30, 4.70 and 2.53 mg/mL, respectively, to BTBF, BTHE and BTCF, which. The phytochemical analysis detected the presence of flavanoids, triterpene, carbohydrate, and tannin. Our work showed for the first time the broad-spread antimicrobial activity of B. tetraphylla, which has nonhemolytic action, creating a new perspective on the interesting association of traditional and scientific knowledge.

  19. "One Long Struggle for Justice": An Interview with Historian Howard Zinn

    ERIC Educational Resources Information Center

    Bigelow, Bill

    2010-01-01

    In 2008 "Rethinking Schools" and the Washington, D.C.-based education nonprofit Teaching for Change joined together to form the Zinn Education Project, dedicated to promoting the teaching of a people's history in middle and high schools throughout the United States. The Zinn Education Project recently launched a new website,…

  20. Howard mold count of fruit nectars, purees, and pastes: collaborative study.

    PubMed

    Cichowicz, S M; Bandler, R

    1982-09-01

    The various mold count methods for nectars, purees, and pastes make no adjustment for concentration of the pulped liquid. Thus, mold counts of different forms of the same product (e.g., guava nectar, puree, or paste) or of the same product from different manufacturers cannot be compared. A method was developed in which the product is centrifuged under standardized conditions, then diluted to a standard pellet-to-final-volume ratio. Because variations in the concentration of fruit or vegetable tissue are corrected, comparisons of mold counts are possible. The method was adopted official first action.

  1. Why Baby Why: Howard Broomfield's Documentation of the Dunne-za Soundscape.

    ERIC Educational Resources Information Center

    Ridington, Robin

    1988-01-01

    Examines the acoustic environment and aural culture of the Dunne-za of northeastern British Columbia. Discusses the changing soundscape of a northern hunting people, techniques for recording and cataloging an ethnographic soundscape archive, and the use of audio actualities in producing ethnographic audio documentaries. (Author/SV)

  2. Lucy Diggs Slowe, Howard University Dean of Women, 1922-1937: Educator Administrator, Activist

    ERIC Educational Resources Information Center

    Rasheed, Lisa R.

    2010-01-01

    Within the last twenty years, some educational researchers initiated an emphasis to study the accomplishments and contributions of African-American women in higher education. Although they were marginally recognized, some African-American women forged into uncharted territories by providing examples of administrative leadership in post-secondary…

  3. Howard Zinn and the Struggle for the Microphone: History, Objectivity, and Citizenship

    ERIC Educational Resources Information Center

    Kelly, Jason M.

    2009-01-01

    Every year, historians in the United States attend the American Historical Association (AHA), a conference that has met annually since 1884. The AHA draws scholars from all specializations, and it is the primary organization through which the profession is represented. In 1969, the conference met at the Sheraton Park Hotel in Washington, D.C. At…

  4. Changes in Research Productivity in Counseling Psychology: Revisiting Howard (1983) a Decade Later.

    ERIC Educational Resources Information Center

    Delgado, Edward A.; Howard, George S.

    1994-01-01

    Assessed institutional research productivity in counseling psychology by totaling credits for articles published from 1983 through 1992 in "Journal of Counseling Psychology,""Counseling Psychologist,""Journal of Consulting and Clinical Psychology,""Journal of Vocational Behavior," and "Journal of Counseling and Development." Found that several…

  5. Embankment Criteria and Performance Report. Cheyenne River Basin, South Dakota, Red Dale Gulch Area, Cedar Canyon Dam, Rapid City, South Dakota.

    DTIC Science & Technology

    1983-01-01

    yards 3. SPILLWAY. Type Uncontrolled-excavated in Minnekahta Limestone Bedrock Location Right abutment of dam Crest Elevation 3554.0 feet m.s.l. Width...embankment in the valley consists of 4 to 10 feet of red clay overburden underlain by Minnekahta lime- stone bedrock. This overburden soil contains a...Protection. Both faces of the dam are protected by rock slope protection to elevation 3554.0 (Crest of Dam). The rock used was excavated Minnekahta limestone

  6. Order Denying the Petition to Object to the Eastern Kentucky Power Coop. William C. Dale Power Station, Ford, Kentucky, Title V Operating Permit

    EPA Pesticide Factsheets

    This document may be of assistance in applying the Title V air operating permit regulations. This document is part of the Title V Petition Database available at www2.epa.gov/title-v-operating-permits/title-v-petition-database. Some documents in the database are a scanned or retyped version of a paper photocopy of the original. Although we have taken considerable effort to quality assure the documents, some may contain typographical errors. Contact the office that issued the document if you need a copy of the original.

  7. Dung pat nesting by the solitary bee, Osmia (Acanthosmiodes) integra (Megachilidae: Apiformes).

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Solitary bees nest in a diversity of substrates, typically soil, but also wood, stems and twigs. A new and novel substrate is reported here, dried cattle dung. Two species of Osmia bees were found nesting in dung in Wyoming. One species, O. integra, otherwise nests shallowly in soil. Nests were ...

  8. RAND’s Portfolio Analysis Tool (PAT): Theory, Methods, and Reference Manual

    DTIC Science & Technology

    2009-01-01

    Strategic planning often seeks to balance investments across numerous objectives. Defense planners, for example, have objectives relating to force...objectives, few, if any, of which can be ignored. Nonetheless, choices must be made, because resources are finite. Consequently, strategic planning often

  9. RF Manipulation and Detection of Protons in the High Performance Antiproton Trap (HiPAT)

    NASA Technical Reports Server (NTRS)

    Martin, James J.; Lewis, Raymond A.; Pearson, J. Boise; Sims, W. Herb; Chakrabarti, Suman; Fant, Wallace E.; McDonald, Stan

    2003-01-01

    The significant energy density of matter-antimatter annihilation is attractive to the designers of future space propulsion systems, with the potential to offer a highly compact source of power. Many propulsion concepts exist that could take advantage of matter-antimatter reactions, and current antiproton production rates are sufficient to support basic proof-of-principle evaluation of technology associated with antimatter-derived propulsion. One enabling technology for such experiments is portable storage of low energy antiprotons, allowing antiprotons to be trapped, stored, and transported for use at an experimental facility.

  10. How Early Events Affect Growing Brains. An Interview with Neuroscientist Pat Levitt

    ERIC Educational Resources Information Center

    National Scientific Council on the Developing Child, 2006

    2006-01-01

    Recent advances in neuroscience show clearly how experience can change brain neurochemicals, and how this in turn affects the way the brain functions. As a result, early negative events actually get built into the growing brain's neurochemistry, altering the brain's architecture. Research is continuing to investigate how children with genetic…

  11. A PAT-based qualification of pharmaceutical excipients produced by batch or continuous processing.

    PubMed

    Hertrampf, A; Müller, H; Menezes, J C; Herdling, T

    2015-10-10

    Pharmaceutical excipients have an influence on the main requirements for medicinal products (viz., quality, safety and efficacy) but also on their manufacturability. During product lifecycle it may become necessary to introduce minor changes (e.g., to continuously improve it) or major changes in the validated process (e.g., moving it to a new production site, replacing process version or even disruptively changing processing type). Those changes can influence the critical to quality attributes of the product. Therefore, it is important to enhance process understanding to avoid the risk of any significant quality changes. Process analytical technology can support better decision making and risk-management as required in quality by design - viz., by many pharmaceutical regulatory authorities. This study compares the quality of the pharmaceutical excipient sodium carbonate (anhydrous) produced either in a batch or a continuous process. For continuous processing two different production lines were available that differed on the dryer and crystallizer types used. Therefore their influence on critical to quality attributes of sodium carbonate was investigated for each of the three processing alternatives. The overall goal was to identify which of the continuous processes ensures a similar product quality to batch processing. Namely, changes on chemical and physical attributes of the product were investigated with Raman spectroscopy, laser diffraction and X-ray powder diffraction. Principal component analysis, a very common multivariate analysis technique, was applied to extract relevant information from small differences at multiple spectral regions from samples from each process type and from each analytical technique used. Changing processing from batch to continuous improved consistency of certain attributes (e.g., particle size distribution) but affected others. However, the increased process/product knowledge gained can lead to an enhanced control strategy and ensure a similar product quality is obtained from distinct process versions.

  12. Design and Preliminary Testing of a High Performance Antiproton Trap (HiPAT)

    NASA Technical Reports Server (NTRS)

    Martin, James; Meyer, Kirby; Kramer, Kevin; Smith, Gerald; Lewis, Raymond; Rodgers, Stephen L. (Technical Monitor)

    2000-01-01

    Antimatter represents the pinnacle of energy density, offering the potential to enhance current fusion/fission concepts enabling various classes of deep space missions. Current production rates are sufficient to support proof-of-concept evaluation of many key technologies associated with antimatter-derived propulsion. Storage has been identified as a key enabling technology for all antimatter-related operations, and as such is the current focus of this NASA-MSFC effort to design and fabricate a portable device capable of holding up to 10(exp 12) particles. Hardware has been assembled and initial tests are underway to evaluate the trap behavior using electron gun generated, positive hydrogen ions. Ions have been stored for tens of minutes, limited by observed interaction with background gas. Additionally, radio frequency manipulation is being tested to increase lifetime by stabilizing the stored particles, potentially reducing their interaction with background gas, easing requirements on ultimate trap vacuum and precision mechanical alignment.

  13. Betty and Pat: Matching Opposites as a Technique toward Community Involvement in Offender Rehabilitation.

    ERIC Educational Resources Information Center

    Gregory, Robert J.

    1996-01-01

    Describes a project in which female prisoners were matched with socially active women volunteers from the surrounding community. Program encouraged home visits, shopping trips, attendance at various activities in the community, and opportunities to engage in personal interaction. Results indicate that each encounter was significant to all persons…

  14. PAT for tableting: inline monitoring of API and excipients via NIR spectroscopy.

    PubMed

    Wahl, Patrick R; Fruhmann, Georg; Sacher, Stephan; Straka, Gerhard; Sowinski, Sebastian; Khinast, Johannes G

    2014-07-01

    This paper describes the application and implementation of inline NIR spectroscopy in an industrial tablet press. The content uniformity of a powder was analyzed via a NIR probe mounted on the feed frame. A PLS model with four latent variables (R(2)=0.97, Q(2)=0.95) was developed for the Active Pharmaceutical Ingredient (API) and two main excipients (EX1, EX2), according to the mixture DoE. The RMSEP corresponded to the relative errors of 2.7% for API, 1.7% for EX1 and 2.6% for EX2, compared to the nominal formulation. Transfer of the model, from the lab to an inline setup for manufacturing was achieved using local centering. There was a good agreement between the results of inline NIR and drawn tablets analyzed via UV-Vis. Notably, NIR indicated stochastic segregation behavior of the powder toward the end of the process, which was confirmed by the UV-Vis analysis. The outcome of our work was related to the recently published Ph. Eur. chapter 2.9.47 "Demonstration of uniformity of dosage units using large sample sizes".

  15. Model-Based PAT for Quality Management in Pharmaceuticals Freeze-Drying: State of the Art

    PubMed Central

    Fissore, Davide

    2017-01-01

    Model-based process analytical technologies can be used for the in-line control and optimization of a pharmaceuticals freeze-drying process, as well as for the off-line design of the process, i.e., the identification of the optimal operating conditions. This paper aims at presenting the state of the art in this field, focusing, particularly, on three groups of systems, namely, those based on the temperature measurement (i.e., the soft sensor), on the chamber pressure measurement (i.e., the systems based on the test of pressure rise and of pressure decrease), and on the sublimation flux estimate (i.e., the tunable diode laser absorption spectroscopy and the valveless monitoring system). The application of these systems for in-line process optimization (e.g., using a model predictive control algorithm) and to get a true quality by design (e.g., through the off-line calculation of the design space of the process) is presented and discussed. PMID:28224123

  16. 40 CFR 174.522 - Phosphinothricin Acetyltransferase (PAT); exemption from the requirement of a tolerance.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ...) enzyme are exempt from the requirement of a tolerance when used as plant-incorporated protectant inert... ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) PESTICIDE PROGRAMS PROCEDURES AND REQUIREMENTS FOR...

  17. Multivariate data analysis as a PAT tool for early bioprocess development data.

    PubMed

    Mercier, Sarah M; Diepenbroek, Bas; Dalm, Marcella C F; Wijffels, Rene H; Streefland, Mathieu

    2013-09-10

    Early development datasets are typically unstructured, incomplete and truncated, yet they are readily available and contain relevant process information which is not extracted using classical data analysis techniques. In this paper, we illustrate the power of multivariate data analysis (MVDA) as a Process Analytical Technology tool to analyze early development data of a PER.C6® cell cultivation process. MVDA increased our understanding of the process studied. Principal component analysis enabled a thorough exploration of the dataset, identifying causes for batch deviations and revealing sensitivity of the process to scale. These findings were previously undetected using traditional univariate analysis. The lack of structure and gaps in the early development datasets made it impossible to fit them to more advanced partial least square regression models. This paper clearly shows that MVDA should be routinely used to analyze early development data to reveal relevant information for later development and scale-up. The value of these early development runs can be greatly enhanced if the experiments are well-structured and accompanied with full process analytics. This up-front investment will result in shorter and more efficient process development paths, resulting in lower overall development costs for new biopharmaceutical products.

  18. 77 FR 76457 - Howard Hughes Medical Institute, et al.; Notice of Consolidated Decision on Applications for Duty...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-12-28

    ... relationships between nanoparticle size, shape and chemical state and their catalytic activity in various chemical reactions, by investigating solid cataltically-active materials such as transition metals and... or chemical modification, in excess or limited water, without shear or at high shear, as well...

  19. Carl Levin and Howard P. "Buck" McKeon National Defense Authorization Act for Fiscal Year 2015

    THOMAS, 113th Congress

    Rep. Barletta, Lou [R-PA-11

    2014-01-31

    12/19/2014 Became Public Law No: 113-291. (TXT | PDF) (All Actions) Notes: See the committee print, containing the legislative text and joint explanatory statement, accompanying the enrolled version of H.R.3979. Tracker: This bill has the status Became LawHere are the steps for Status of Legislation:

  20. 77 FR 20009 - Howard Hughes Medical Institute, et al.; Notice of Consolidated Decision on Applications for Duty...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-04-03

    ... Applications for Duty-Free Entry of Electron Microscope This is a decision consolidated pursuant to Section 6(c...: Electron Microscope. Manufacturer: FEI Company, Czech Republic. Intended Use: See notice at 77 FR 12240..., AR 72079. Instrument: Electron Microscope. Manufacturer: JEOL Instruments, Japan. Intended Use:...

  1. A resolution relative to the death of Howard O. Greene, Jr., former Sergeant at Arms of the United States Senate.

    THOMAS, 113th Congress

    Sen. McConnell, Mitch [R-KY

    2014-11-17

    11/17/2014 Submitted in the Senate, considered, and agreed to without amendment and with a preamble by Unanimous Consent. (consideration: CR S6005; text as passed Senate: CR S6024) (All Actions) Tracker: This bill has the status Passed SenateHere are the steps for Status of Legislation:

  2. 75 FR 63166 - Howard Rosenfeld; Notice of Declaration of Intention and Soliciting Comments, Protests, and/or...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-10-14

    .... Name of Project: Warren Energy Independence Hydroelectric Project. f. Location: The proposed Warren Energy Independence Hydroelectric Project will be located on Sucker Brook, a.k.a. Lake Waramaug Brook..., protests, and/or motions filed. k. Description of Project: The proposed Warren Energy...

  3. Dr. Howard L. Simmons: An Intellectual Biography of the First Black Executive Director of the Middle States Regional Accrediting Body

    ERIC Educational Resources Information Center

    Mendoza, Cynthia

    2009-01-01

    There is limited information on changes to accreditation standards and practices, especially related to diversity. The narrow focus of prior studies and the public's limited understanding of accreditation and its criteria have contributed to this gap in the literature. This qualitative study used intellectual biography as its framework to narrate…

  4. HIV medicine after Barcelona conference: interview with Howard Grossman, M.D. Interview by John S. James.

    PubMed

    Grossman, Howard

    2002-09-06

    We asked a leading HIV physician to summarize some of the treatment issues that clinicians are now looking at after the Barcelona conference. Topics in this interview include: Tenofovir, including first-line therapy; The shift from protease inhibitors to non-nucleoside RT inhibitors, for many but not all patients; Benefits of once-a-day regimens; Lipodystrophy, and strategies for avoiding or reducing it; Viral resistance testing; T-20; Prevention--including danger of superinfection with a new HIV strain.

  5. Carl Levin and Howard P. "Buck" McKeon National Defense Authorization Act for Fiscal Year 2015

    THOMAS, 113th Congress

    Rep. Barletta, Lou [R-PA-11

    2014-01-31

    12/19/2014 Became Public Law No: 113-291. (PDF) (All Actions) Notes: See the committee print, containing the legislative text and joint explanatory statement, accompanying the enrolled version of H.R.3979. Tracker: This bill has the status Became LawHere are the steps for Status of Legislation:

  6. 'Don't save her'- Sigmund Freud meets Project Pat: the rescue motif in hip-hop.

    PubMed

    Fulmer, Richard H

    2008-08-01

    Freud originally explicated the dynamics of rescue wishes by describing men who fell in love with prostitutes. He saw this attempt at attachment as driven by the man's wish to repay his parents for giving him life. Many subsequent writers shift this emphasis, seeing rescue wishes as motivated by aggressive oedipal competition with the father. This article highlights the attachment aspects of Freud's original conception and traces how writers in the last three decades use the family romance rather than Oedipus as a model to view rescue wishes as having a more tender aspect. Rescue wishes are especially characteristic of the developmental stage of young adulthood. They at once attempt to repay the parental debt the young adult feels and serve as practice for the vicissitudes of the couple bond and the benign sacrifices of parenthood. Popular culture contains many vivid examples of Freud 's original description of the rescue-motif. A detailed examination of the rap song, Don't save her, demonstrates all the elements of Freud's original conception and the interpersonal risks of the rescue relationship. Three additional rap songs by other artists are briefly analyzed to show the extensive occurrence of the wish to rescue and its psychic and interpersonal dangers.

  7. Focused beam reflectance method as an innovative (PAT) tool to monitor in-line granulation process in fluidized bed.

    PubMed

    Alshihabi, Firas; Vandamme, Thierry; Betz, Gabriele

    2013-02-01

    Fluidized bed granulation is a commonly used unit operation in the pharmaceutical industry. But still to obtain and control the desired granule size is challenging due to many process variables affecting the final product. Focused beam reflectance measurement (FBRM, Mettler-Toledo, Switzerland) is an increasingly popular particle growth analysis technique. FBRM tool was installed in two different locations inside a fluidized bed granulator (GPCG2, Glatt, Binzen) in order to monitor the granulation growth kinetics. An experimental design was created to study the effect of process variables using FBRM probe and comparing the results with the one's measured by sieve analysis. The probe location is of major importance to get smooth and robust curves. The excess feeding of binder solution might lead to agglomeration and thus to process collapse, however this phenomenon was clearly detected with FBRM method. On the other hand, the process variables at certain levels might affect the FBRM efficiency by blocking the probe window with sticky particles. A good correlation was obtained (R(2) = 0.95) between FBRM and sieve analysis mean particle size. The proposed in-line monitoring tool enables the operator to select appropriate process parameters and control the wet granulation process more efficiently.

  8. Hybrid modeling as a QbD/PAT tool in process development: an industrial E. coli case study.

    PubMed

    von Stosch, Moritz; Hamelink, Jan-Martijn; Oliveira, Rui

    2016-05-01

    Process understanding is emphasized in the process analytical technology initiative and the quality by design paradigm to be essential for manufacturing of biopharmaceutical products with consistent high quality. A typical approach to developing a process understanding is applying a combination of design of experiments with statistical data analysis. Hybrid semi-parametric modeling is investigated as an alternative method to pure statistical data analysis. The hybrid model framework provides flexibility to select model complexity based on available data and knowledge. Here, a parametric dynamic bioreactor model is integrated with a nonparametric artificial neural network that describes biomass and product formation rates as function of varied fed-batch fermentation conditions for high cell density heterologous protein production with E. coli. Our model can accurately describe biomass growth and product formation across variations in induction temperature, pH and feed rates. The model indicates that while product expression rate is a function of early induction phase conditions, it is negatively impacted as productivity increases. This could correspond with physiological changes due to cytoplasmic product accumulation. Due to the dynamic nature of the model, rational process timing decisions can be made and the impact of temporal variations in process parameters on product formation and process performance can be assessed, which is central for process understanding.

  9. Peanut Allergen Threshold Study (PATS): validation of eliciting doses using a novel single-dose challenge protocol

    PubMed Central

    2013-01-01

    Background The eliciting dose (ED) for a peanut allergic reaction in 5% of the peanut allergic population, the ED05, is 1.5 mg of peanut protein. This ED05 was derived from oral food challenges (OFC) that use graded, incremental doses administered at fixed time intervals. Individual patients’ threshold doses were used to generate population dose-distribution curves using probability distributions from which the ED05 was then determined. It is important to clinically validate that this dose is predictive of the allergenic response in a further unselected group of peanut-allergic individuals. Methods/Aims This is a multi-centre study involving three national level referral and teaching centres. (Cork University Hospital, Ireland, Royal Children’s Hospital Melbourne, Australia and Massachusetts General Hospital, Boston, U.S.A.) The study is now in process and will continue to run until all centres have recruited 125 participates in each respective centre. A total of 375 participants, aged 1–18 years will be recruited during routine Allergy appointments in the centres. The aim is to assess the precision of the predicted ED05 using a single dose (6 mg peanut = 1.5 mg of peanut protein) in the form of a cookie. Validated Food Allergy related Quality of Life Questionnaires-(FAQLQ) will be self-administered prior to OFC and 1 month after challenge to assess the impact of a single dose OFC on FAQL. Serological and cell based in vitro studies will be performed. Conclusion The validation of the ED05 threshold for allergic reactions in peanut allergic subjects has potential value for public health measures. The single dose OFC, based upon the statistical dose-distribution analysis of past challenge trials, promises an efficient approach to identify the most highly sensitive patients within any given food-allergic population. PMID:24028324

  10. Process analytical technology (PAT) in insect and mammalian cell culture processes: dielectric spectroscopy and focused beam reflectance measurement (FBRM).

    PubMed

    Druzinec, Damir; Weiss, Katja; Elseberg, Christiane; Salzig, Denise; Kraume, Matthias; Pörtner, Ralf; Czermak, Peter

    2014-01-01

    Modern bioprocesses demand for a careful definition of the critical process parameters (CPPs) already during the early stages of process development in order to ensure high-quality products and satisfactory yields. In this context, online monitoring tools can be applied to recognize unfavorable changes of CPPs during the production processes and to allow for early interventions in order to prevent losses of production batches due to quality issues. Process analytical technologies such as the dielectric spectroscopy or focused beam reflectance measurement (FBRM) are possible online monitoring tools, which can be applied to monitor cell growth as well as morphological changes. Since the dielectric spectroscopy only captures cells with intact cell membranes, even information about dead cells with ruptured or leaking cell membranes can be derived. The following chapter describes the application of dielectric spectroscopy on various virus-infected and non-infected cell lines with respect to adherent as well as suspension cultures in common stirred tank reactors. The adherent mammalian cell lines Vero (African green monkey kidney cells) and hMSC-TERT (telomerase-immortalized human mesenchymal stem cells) are thereby cultured on microcarrier, which provide the required growth surface and allow the cultivation of these cells even in dynamic culture systems. In turn, the insect-derived cell lines S2 and Sf21 are used as examples for cells typically cultured in suspension. Moreover, the FBRM technology as a further monitoring tool for cell culture applications has been included in this chapter using the example of Drosophila S2 insect cells.

  11. Decay of Fecal Indicator Bacterial Populations and Bovine-Associated Source-Tracking Markers in Freshly Deposited Cow Pats

    EPA Science Inventory

    Understanding the survival of fecal indicator bacteria (FIB) and microbial source-tracking (MST) markers is critical to developing pathogen fate and transport models. Although pathogen survival in water microcosms and manure-amended soils is well documented, little is known about...

  12. Fast freeze-drying cycle design and optimization using a PAT based on the measurement of product temperature.

    PubMed

    Bosca, Serena; Barresi, Antonello A; Fissore, Davide

    2013-10-01

    This paper is focused on the use of an innovative Process Analytical Technology for the fast design and optimization of freeze-drying cycles for pharmaceuticals. The tool is based on a soft-sensor, a device that uses the experimental measure of product temperature during freeze-drying, a mathematical model of the process, and the Extended Kalman Filter algorithm to estimate the sublimation flux, the residual amount of ice in the vial, and some model parameters (heat and mass transfer coefficients). The accuracy of the estimations provided by the soft-sensor has been shown using as test case aqueous solutions containing different excipients (sucrose, polyvinylpyrrolidone), processed at various operating conditions, pointing out that the soft-sensor allows a fast estimation of model parameters and product dynamics without involving expensive hardware or time consuming analysis. The possibility of using the soft-sensor to calculate in-line (or off-line) the design space of the primary drying phase is here presented and discussed. Results evidences that by this way, it is possible to identify the values of the heating fluid temperature that maintain product temperature below the limit value, as well as the operating conditions that maximize the sublimation flux. Various experiments have been carried out to test the effectiveness of the proposed approach for a fast design of the cycle, evidencing that drying time can be significantly reduced, without impairing product quality.

  13. To designate the Federal building and United States courthouse located at 224 South Boulder Avenue in Tulsa, Oklahoma, as the "H. Dale Cook Federal Building and United States Courthouse".

    THOMAS, 111th Congress

    Rep. Sullivan, John [R-OK-1

    2009-07-23

    11/18/2009 Received in the Senate and Read twice and referred to the Committee on Environment and Public Works. (All Actions) Tracker: This bill has the status Passed HouseHere are the steps for Status of Legislation:

  14. Étude expérimentale du dépôt de particules colloïdales en milieu poreux : Influence de l'hydrodynamique et de la salinité

    NASA Astrophysics Data System (ADS)

    Djehiche, Abdelkader; Canseco, Vladimir; Omari, Aziz; Bertin, Henri

    2009-09-01

    This study deals with colloid transport in porous media which applications are found in subsurface water, petroleum engineering or civil engineering. An experimental study of colloidal polystyrene Latex particles deposition in a consolidated porous medium is presented. The influence of ionic strength of the colloid suspension and the flow rate on particle deposition is investigated. We see first that beyond a critical salt concentration, the total collector efficiency increases with the ionic strength. Moreover, such collector efficiency decreases as the flow rate increases according to theory. In other respects, using a γ ray attenuation technique allows us to measure local porosity fluctuation due to particles deposition. By this way deposition kinetics may be followed locally and precisely. Nevertheless when considering the thickness of the adsorbed layer over large scales, obtained results using the γ rays attenuation technique are found in good agreement with those obtained by means of an usual technique especially at latest stages of adsorption process. To cite this article: A. Djehiche et al., C. R. Mecanique 337 (2009).

  15. Scientific analysis is essential to assess biofuel policy effects: in response to the paper by Kim and Dale on "Indirect land use change for biofuels: Testing predictions and improving analytical methodologies"

    SciTech Connect

    Kline, Keith L; Oladosu, Gbadebo A; Dale, Virginia H; McBride, Allen

    2011-01-01

    Vigorous debate on the effects of biofuels derives largely from the changes in land use estimated using economic models designed mainly for the analysis of agricultural trade and markets. The models referenced for land-use change (LUC) analysis in the U.S. Environmental Protection Agency Final Rule on the Renewable Fuel Standard include GTAP, FAPRI-CARD, and FASOM. To address bioenergy impacts, these models were expanded and modified to facilitate simulations of hypothesized LUC. However, even when models use similar basic assumptions and data, the range of LUC results can vary by ten-fold or more. While the market dynamics simulated in these models include processes that are important in estimating effects of biofuel policies, the models have not been validated for estimating land-use changes and employ crucial assumptions and simplifications that contradict empirical evidence.

  16. National Dam Safety Program. Elliot Davis Lake Dam (MO 10129), Missouri - Kansas City Basin, Howard County, Missouri. Phase I Inspection Report.

    DTIC Science & Technology

    1978-09-01

    1 .9 I I 1 I 1 ~ C aa ~ ~fl~ifltfl@Q.4d... ~C.4aCJ~. i Ca a a aa 000 1 aaaaaaaaa I Ceaaaa000 CO 000001 I A 1. O@b@OOO@@ - I I 𔄃oeae~p- EuQ I, I I I...LO f,~ -~ on- 21%0 ’ 0" N4 02 p2 . mw X C10. MN r-m tN AI 40’ o C O M P U T tD B ~ r S M E N o O F/ fosKINS-WIESTERN-SONDEREGGER sHCCOI AE______

  17. An old remedy for a new problem? Identification of Ooencyrtus kuvanae (Howard) (Hymenoptera: Encyrtidae) as egg parasitoids of spotted lanternfly, Lycorma delicatula (Hemiptera: Fulgoridae) in North America

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Spotted Lanternfly (SLF), Lycorma delicatula (White) is a recently introduced pest of Tree-of-Heaven, Ailanthus altissima (Mill.) Swingle in North America. Natural enemy surveys for this pest in Pennsylvania in 2016 recovered an encyrtid egg parasitoid from both field collections and laboratory rear...

  18. President Franklin D Roosevelt (1882-1945) and Doctor Frank Howard Lahey's (1880-1953) dilemma: The complexities of medical confidentiality with World Leaders.

    PubMed

    Steinberg, David

    2016-02-01

    In the months before the Presidential Election of November 1944, there were serious concerns about the health of President Franklin D Roosevelt. He had lost considerable weight and his once robust appearance had given way to a tired and haggard look. Despite these worrisome observations, Vice-Admiral Ross T McIntire, who served as Franklin Roosevelt's personal physician from 1933 until Roosevelt's death, lied when he proclaimed Roosevelt to be in 'excellent condition for a man of his age'. The truth about Roosevelt's health was further obscured when Roosevelt's medical records disappeared.

  19. Anthropophilic biting behaviour of Anopheles (Kerteszia) neivai Howard, Dyar & Knab associated with Fishermen's activities in a malaria-endemic area in the Colombian Pacific.

    PubMed

    Escovar, Jesús Eduardo; González, Ranulfo; Quiñones, Martha Lucía

    2013-12-01

    On the southwest Pacific Coast of Colombia, a field study was initiated to determine the human-vector association between Anopheles (Kerteszia) neivai and fishermen, including their nearby houses. Mosquitoes were collected over 24-h periods from mangrove swamps, marshlands and fishing vessels in three locations, as well as in and around the houses of fishermen. A total of 6,382 mosquitoes were collected. An. neivai was most abundant in mangroves and fishing canoes (90.8%), while Anopheles albimanus was found indoors (82%) and outdoors (73%). One An. neivai and one An. albimanus collected during fishing activities in canoes were positive for Plasmodium vivax , whereas one female An. neivai collected in a mangrove was positive for P. vivax . In the mangroves and fishing canoes, An. neivai demonstrated biting activity throughout the day, peaking between 06:00 pm-07:00 pm and there were two minor peaks at dusk and dawn. These peaks coincided with fishing activities in the marshlands and mangroves, a situation that places the fishermen at risk of contracting malaria when they are performing their daily activities. It is recommended that protective measures be implemented to reduce the risk that fishermen will contract malaria.

  20. Stockton Lake Wimmer Collections. Analysis of Prehistoric Artifacts Collected by Howard R. Wimmer from Archeological Sites in the Stockton Lake Project, Cedar and Dade Counties, Missouri

    DTIC Science & Technology

    1993-01-01

    tools were allegedly used by pre- Clovis peoples in the Americas (Chapman 1975:31-59; Wright 1987:C-1-1 - C-1-3). The well-made projectile points...associated with Clovis and later cultures supposedly were not made by these peoples. The alleged Lively Complex of Alabama (Josselyn 1965) and Byran’s (1965...Dalton component found at Montgomery Paloe-Indian 12,000 • 8(X𔃽 BC only a few Clovis Fluted asd Folsom Flu•td Early Man Pre-12.000 BC no data Paleo

  1. Description of a new species of Anagyrus Howard (Hymenoptera: Chalcidoidea: Encyrtidae), a promising biological control agent of the invasive Madeira mealybug, Phenacoccus madeirensis Green (Hemiptera: Sternorrhyncha: Pseudococcidae).

    PubMed

    Rameshkumar, A; Noyes, J S; Poorani, J; Chong, J H

    2013-01-01

    Anagyrus amnestos sp. n. (Hymenoptera: Encyrtidae), a promising parasitoid of the invasive Madeira mealybug, Phenacoccus madeirensis Green (Hemiptera: Pseudococcidae), is described based on material collected from India. This parasitoid was identified as Anagyrus sp. nov. nr. sinope Noyes & Menezes in recent literature, and was initially collected in Georgia, USA. It was found to be a specific parasitoid of the Madeira mealybug and its biological attributes and potential as a biological control agent of this pest were studied. In what appears to be a case of fortuitous introduction, we detected this parasitoid in large numbers on Madeira mealybugs from the southern Indian state of Karnataka, where the mealybug is a recently introduced invasive pest. In view of its economic importance as a potential biological control agent of the Madeira mealybug, it is formally described and illustrated here. Comparative accounts of the new species vis-a-vis its close relatives in India and the Americas are provided.

  2. Geologic and Fossil Locality Maps of the West-Central Part of the Howard Pass Quadrangle and Part of the Adjacent Misheguk Mountain Quadrangle, Western Brooks Range, Alaska

    USGS Publications Warehouse

    Dover, James H.; Tailleur, Irvin L.; Dumoulin, Julie A.

    2004-01-01

    The map depicts the field distribution and contact relations between stratigraphic units, the tectonic relations between major stratigraphic sequences, and the detailed internal structure of these sequences. The stratigraphic sequences formed in a variety of continental margin depositional environments, and subsequently underwent a complexde formational history of imbricate thrust faulting and folding. A compilation of micro and macro fossil identifications is included in this data set.

  3. Aeromagnetic survey of Howard Pass quadrangle and the East half of Misheguk Mountain quadrangle, Alaska—a Web site for the distribution of data

    USGS Publications Warehouse

    Brown, Philip J.

    2009-01-01

    U.S. Geological Survey Open-File-Report 2009-1256 is for the preliminary release of magnetic data (and associated contractor reports) for an airborne survey in the Brooks Range, northwest of Bettles, Alaska.

  4. Anthropophilic biting behaviour of Anopheles (Kerteszia) neivai Howard, Dyar & Knab associated with Fishermen’s activities in a malaria-endemic area in the Colombian Pacific

    PubMed Central

    Escovar, Jesús Eduardo; González, Ranulfo; Quiñones, Martha Lucía

    2013-01-01

    On the southwest Pacific Coast of Colombia, a field study was initiated to determine the human-vector association between Anopheles (Kerteszia) neivai and fishermen, including their nearby houses. Mosquitoes were collected over 24-h periods from mangrove swamps, marshlands and fishing vessels in three locations, as well as in and around the houses of fishermen. A total of 6,382 mosquitoes were collected. An. neivai was most abundant in mangroves and fishing canoes (90.8%), while Anopheles albimanus was found indoors (82%) and outdoors (73%). One An. neivai and one An. albimanus collected during fishing activities in canoes were positive for Plasmodium vivax , whereas one female An. neivai collected in a mangrove was positive for P. vivax . In the mangroves and fishing canoes, An. neivai demonstrated biting activity throughout the day, peaking between 06:00 pm-07:00 pm and there were two minor peaks at dusk and dawn. These peaks coincided with fishing activities in the marshlands and mangroves, a situation that places the fishermen at risk of contracting malaria when they are performing their daily activities. It is recommended that protective measures be implemented to reduce the risk that fishermen will contract malaria. PMID:24402159

  5. 78 FR 13883 - Federal Property Suitable as Facilities To Assist the Homeless

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-03-01

    ... needed; asbestos; w/in controlled area; contact Ranger District for accessibility Dale Duplex (1057...; major repairs needed; asbestos Dale Residence (1059) 48743 Hwy 395 N. Dale OR 97880 Landholding Agency...; major repairs needed; asbestos; w/in controlled area; contact Ranger District for accessibility...

  6. Real-time polymerase chain reaction-based approach for quantification of the pat gene in the T25 Zea mays event.

    PubMed

    Weighardt, Florian; Barbati, Cristina; Paoletti, Claudia; Querci, Maddalena; Kay, Simon; De Beuckeleer, Marc; Van den Eede, Guy

    2004-01-01

    In Europe, a growing interest for reliable techniques for the quantification of genetically modified component(s) of food matrixes is arising from the need to comply with the European legislative framework on novel food products. Real-time polymerase chain reaction (PCR) is currently the most powerful technique for the quantification of specific nucleic acid sequences. Several real-time PCR methodologies based on different molecular principles have been developed for this purpose. The most frequently used approach in the field of genetically modified organism (GMO) quantification in food or feed samples is based on the 5'-3'-exonuclease activity of Taq DNA polymerase on specific degradation probes (TaqMan principle). A novel approach was developed for the establishment of a TaqMan quantification system assessing GMO contents around the 1% threshold stipulated under European Union (EU) legislation for the labeling of food products. The Zea mays T25 elite event was chosen as a model for the development of the novel GMO quantification approach. The most innovative aspect of the system is represented by the use of sequences cloned in plasmids as reference standards. In the field of GMO quantification, plasmids are an easy to use, cheap, and reliable alternative to Certified Reference Materials (CRMs), which are only available for a few of the GMOs authorized in Europe, have a relatively high production cost, and require further processing to be suitable for analysis. Strengths and weaknesses of the use of novel plasmid-based standards are addressed in detail. In addition, the quantification system was designed to avoid the use of a reference gene (e.g., a single copy, species-specific gene) as normalizer, i.e., to perform a GMO quantification based on an absolute instead of a relative measurement. In fact, experimental evidences show that the use of reference genes adds variability to the measurement system because a second independent real-time PCR-based measurement must be performed. Moreover, for some reference genes no sufficient information on copy number in and among genomes of different lines is available, making adequate quantification difficult. Once developed, the method was subsequently validated according to IUPAC and ISO 5725 guidelines. Thirteen laboratories from 8 EU countries participated in the trial. Eleven laboratories provided results complying with the predefined study requirements. Repeatability (RSDr) values ranged from 8.7 to 15.9%, with a mean value of 12%. Reproducibility (RSDR) values ranged from 16.3 to 25.5%, with a mean value of 21%. Following Codex Alimentarius Committee guidelines, both the limits of detection and quantitation were determined to be <0.1%.

  7. Preparation of Power Distribution System for High Penetration of Renewable Energy Part I. Dynamic Voltage Restorer for Voltage Regulation Pat II. Distribution Circuit Modeling and Validation

    NASA Astrophysics Data System (ADS)

    Khoshkbar Sadigh, Arash

    Part I: Dynamic Voltage Restorer In the present power grids, voltage sags are recognized as a serious threat and a frequently occurring power-quality problem and have costly consequence such as sensitive loads tripping and production loss. Consequently, the demand for high power quality and voltage stability becomes a pressing issue. Dynamic voltage restorer (DVR), as a custom power device, is more effective and direct solutions for "restoring" the quality of voltage at its load-side terminals when the quality of voltage at its source-side terminals is disturbed. In the first part of this thesis, a DVR configuration with no need of bulky dc link capacitor or energy storage is proposed. This fact causes to reduce the size of the DVR and increase the reliability of the circuit. In addition, the proposed DVR topology is based on high-frequency isolation transformer resulting in the size reduction of transformer. The proposed DVR circuit, which is suitable for both low- and medium-voltage applications, is based on dc-ac converters connected in series to split the main dc link between the inputs of dc-ac converters. This feature makes it possible to use modular dc-ac converters and utilize low-voltage components in these converters whenever it is required to use DVR in medium-voltage application. The proposed configuration is tested under different conditions of load power factor and grid voltage harmonic. It has been shown that proposed DVR can compensate the voltage sag effectively and protect the sensitive loads. Following the proposition of the DVR topology, a fundamental voltage amplitude detection method which is applicable in both single/three-phase systems for DVR applications is proposed. The advantages of proposed method include application in distorted power grid with no need of any low-pass filter, precise and reliable detection, simple computation and implementation without using a phased locked loop and lookup table. The proposed method has been verified by simulation and experimental tests under various conditions considering all possible cases such as different amounts of voltage sag depth (VSD), different amounts of point-on-wave (POW) at which voltage sag occurs, harmonic distortion, line frequency variation, and phase jump (PJ). Furthermore, the ripple amount of fundamental voltage amplitude calculated by the proposed method and its error is analyzed considering the line frequency variation together with harmonic distortion. The best and worst detection time of proposed method were measured 1ms and 8.8ms, respectively. Finally, the proposed method has been compared with other voltage sag detection methods available in literature. Part 2: Power System Modeling for Renewable Energy Integration: As power distribution systems are evolving into more complex networks, electrical engineers have to rely on software tools to perform circuit analysis. There are dozens of powerful software tools available in the market to perform the power system studies. Although their main functions are similar, there are differences in features and formatting structures to suit specific applications. This creates challenges for transferring power system circuit models data (PSCMD) between different software and rebuilding the same circuit in the second software environment. The objective of this part of thesis is to develop a Unified Platform (UP) to facilitate transferring PSCMD among different software packages and relieve the challenges of the circuit model conversion process. UP uses a commonly available spreadsheet file with a defined format, for any home software to write data to and for any destination software to read data from, via a script-based application called PSCMD transfer application. The main considerations in developing the UP are to minimize manual intervention and import a one-line diagram into the destination software or export it from the source software, with all details to allow load flow, short circuit and other analyses. In this study, ETAP, OpenDSS, and GridLab-D are considered, and PSCMD trans

  8. Two Decades of Immigration: Has the Sky Fallen Yet? Pat Brown Institute of Public Affairs State of Immigration Report. Volume 1.

    ERIC Educational Resources Information Center

    Modarres, Ali

    This report provides a demographic analysis of immigration to the United States and California, using information from the Immigration and Naturalization Service annual reports and digital database for 1990-98. It features details on the Asian immigrant population for the 1990s. From 1820-1998, about 62 million immigrants came to America. Nearly…

  9. Content uniformity determination of pharmaceutical tablets using five near-infrared reflectance spectrometers: a process analytical technology (PAT) approach using robust multivariate calibration transfer algorithms.

    PubMed

    Sulub, Yusuf; LoBrutto, Rosario; Vivilecchia, Richard; Wabuyele, Busolo Wa

    2008-03-24

    Near-infrared calibration models were developed for the determination of content uniformity of pharmaceutical tablets containing 29.4% drug load for two dosage strengths (X and Y). Both dosage strengths have a circular geometry and the only difference is the size and weight. Strength X samples weigh approximately 425 mg with a diameter of 12 mm while strength Y samples, weigh approximately 1700 mg with a diameter of 20mm. Data used in this study were acquired from five NIR instruments manufactured by two different vendors. One of these spectrometers is a dispersive-based NIR system while the other four were Fourier transform (FT) based. The transferability of the optimized partial least-squares (PLS) calibration models developed on the primary instrument (A) located in a research facility was evaluated using spectral data acquired from secondary instruments B, C, D and E. Instruments B and E were located in the same research facility as spectrometer A while instruments C and D were located in a production facility 35 miles away. The same set of tablet samples were used to acquire spectral data from all instruments. This scenario mimics the conventional pharmaceutical technology transfer from research and development to production. Direct cross-instrument prediction without standardization was performed between the primary and each secondary instrument to evaluate the robustness of the primary instrument calibration model. For the strength Y samples, this approach was successful for data acquired on instruments B, C, and D producing root mean square error of prediction (RMSEP) of 1.05, 1.05, and 1.22%, respectively. However for instrument E data, this approach was not successful producing an RMSEP value of 3.40%. A similar deterioration was observed for the strength X samples, with RMSEP values of 2.78, 5.54, 3.40, and 5.78% corresponding to spectral data acquired on instruments B, C, D, and E, respectively. To minimize the effect of instrument variability, calibration transfer techniques such as piecewise direct standardization (PDS) and wavelet hybrid direct standardization (WHDS) were used. The PDS approach, the RMSEP values for strength X samples were lowered to 1.22, 1.12, 1.19, and 1.08% for instruments B, C, D, and E, respectively. Similar improvements were obtained using the WHDS approach with RMSEP values of 1.36, 1.42, 1.36, and 0.98% corresponding to instruments B, C, D, and E, respectively.

  10. Heat Transfer Analysis of an Optimized, Flexible Holder System for Freeze-Drying in Dual Chamber Cartridges Using Different State-of-the-Art PAT Tools.

    PubMed

    Korpus, Christoph; Pikal, Michael; Friess, Wolfgang

    2016-11-01

    The aim of this study was to determine the heat transfer characteristics of an optimized flexible holder device, using Tunable Diode Laser Absorption Spectroscopy, the Pressure Rise Test, and the gravimetric procedure. Two different controlled nucleation methods were tested, and an improved sublimation process, "preheated plate," was developed. Tunable Diode Laser Absorption Spectroscopy identified an initial sublimation burst phase. Accordingly, steady-state equations were adapted for the gravimetric procedure, to account for this initial non-steady-state period. The heat transfer coefficient, KDCC, describing the transfer from the holder to the DCC, was the only heat transfer coefficient showing a clear pressure dependence with values ranging from 3.81E-04 cal/(g·cm(2)·K) at 40 mTorr to 7.38E-04 cal/(g·cm(2)·K) at 200 mTorr. The heat transfer coefficient, Ktot, reflecting the overall energy transfer via the holder, increased by around 24% from 40 to 200 mTorr. This resulted in a pressure-independent sublimation rate of around 42 ± 1.06 mg/h over the whole pressure range. Hence, this pressure-dependent increase in energy transfer completely compensated the decrease in driving force of sublimation. The "flexible holder" shows a substantially reduced impact of atypical radiation, improved drying homogeneity, and ultimately a better transferability of the freeze-drying cycle for process optimization.

  11. Down on the Bayou--Dr. Pat Honors Us All in Lafayette: A Lifetime of Learning, Laughter, and Love in Louisiana.

    ERIC Educational Resources Information Center

    Thames, Kathleen

    2001-01-01

    Describes the work of Dr. Patricia Rickels, who runs the English Honors program at the University of Louisiana at Lafayette. Discusses how she has also been a matchmaker, a banker, and a shoulder to cry on for students during three decades of change. (Author/EV)

  12. Application of terahertz pulse imaging as PAT tool for non-destructive evaluation of film-coated tablets under different manufacturing conditions.

    PubMed

    Dohi, Masafumi; Momose, Wataru; Yoshino, Hiroyuki; Hara, Yuko; Yamashita, Kazunari; Hakomori, Tadashi; Sato, Shusaku; Terada, Katsuhide

    2016-02-05

    Film-coated tablets (FCTs) are a popular solid dosage form in pharmaceutical industry. Manufacturing conditions during the film-coating process affect the properties of the film layer, which might result in critical quality problems. Here, we analyzed the properties of the film layer using a non-destructive approach with terahertz pulsed imaging (TPI). Hydrophilic tablets that become distended upon water absorption were used as core tablets and coated with film under different manufacturing conditions. TPI-derived parameters such as film thickness (FT), film surface reflectance (FSR), and interface density difference (IDD) between the film layer and core tablet were affected by manufacturing conditions and influenced critical quality attributes of FCTs. Relative standard deviation of FSR within tablets correlated well with surface roughness. Tensile strength could be predicted in a non-destructive manner using the multivariate regression equation to estimate the core tablet density by film layer density and IDD. The absolute value of IDD (Lateral) correlated with the risk of cracking on the lateral film layer when stored in a high-humidity environment. Further, in-process control was proposed for this value during the film-coating process, which will enable a feedback control system to be applied to process parameters and reduced risk of cracking without a stability test.

  13. Process monitoring with disposable chemical sensors fit in the framework of process analysis technology (PAT) for innovative pharmaceutical development and quality assurance.

    PubMed

    Spichiger, Stefan; Spichiger-Keller, Ursula E

    2010-01-01

    The innovative principle of enzymatic sensors applied to monitor the feeding process in disposable bioreactors is described. Innovative is the type of enzyme immobilized within the 'paste' to monitor L-glutamate. Innovative is the application of the miniaturized disposable sensor developed at C-CIT AG for continuous monitoring. The sensor allows the amount of the digested nutrient to be estimated from the amperometric signal. Innovative is the wireless signal transduction between the sensor mounted to the bioreactor and the signal receiver. An example of a process control run is given and, also, the biocompatibility and the specifications of the biosensors. The comparison of results evaluated by different analytical methods is discussed.

  14. The Long History of Old Age The Long History of Old Age Thane Pat Thames & Hudson £25 320 0 500 25126 6 0500251266 [Formula: see text].

    PubMed

    2006-01-01

    This book provides an absorbing overview of 'old age', charting the history of ageing within society. It aims to right the misconceptions of ageing throughout history by writing about topics that have been 'for too long surrounded by taboo' and by challenging some of the misconceptions associated with getting older.

  15. A Research Program in Computer Technology

    DTIC Science & Technology

    1975-09-01

    Moore Vernon W. Reynolds Dale S. Russell Support Staff: Ralph W. Caldwell Wanda N. Canillas Dale M. Chase Oralio E. Carza Delia A. Heilig Kyle P. Lemmons...Raymond L Mason Danny Cohen Marion McKinley Jr. Martin,’. Cohen William H. Moore E. Randolph Cole Vernon W. Reynolds Stephen D. Crocker Dale S. Russell...Ronald L. Currier Thomas 0. Ellis Support Staff: Robert Blechen Lawrence M. Fagar, Ralph W. Caldwell Louis Gallenson Wanda N. Canillas Joel Coldbe’rg

  16. Ecology of Alcohol and Other Drug Use: Helping Black High-Risk Youth. Proceedings of the Howard University School of Human Ecology Forum (Washington, D.C., October 26-27, 1987). OSAP Prevention Monograph-7.

    ERIC Educational Resources Information Center

    Oyemade, Ura Jean, Ed.; Brandon-Monye, Deloris, Ed.

    Five plenary session presentations and summaries of 10 panel sessions held at a forum entitled "The Ecology of Substance Abuse: Toward Primary Prevention among High-Risk Youth" are provided in this document, which focuses on black youth at high risk for alcohol and drug problems. Experts describe a comprehensive ecological approach to addressing…

  17. Comparative analysis of three scale insect species populations (Hemiptera: Coccoidea: Diaspididae) and their parasitoid, Aphytis diaspidis(Howard) on pear trees in Burg el-Arab area, Alexandria, Egypt.

    PubMed

    Mesbah, H A; Mourad, A K; Khadiga, S Moursi; Aziza, A S Fata; Zaghloul, O A; Abdel-Razak, S I

    2009-01-01

    The infestation abundance of each of the inspected scale insect species: San Jose scale, Diaspidiotus perniciosus (Comstock), Greedy scale, Hemiberlisio rapax (Comstock) and Olive scale, Parlatoria oleae (Cloveé) on pear trees, more or less varied as the months of each growing season differed. The comparative abundance percentages in both seasons of 2004-2005 and 2005-2006 were (51.80 and 51.40), (41.20 and 40.90) and (7.10 and 7.70) of the counted individuals of the grand total of H. rapax, D. perniciosus and P. oleae, in respect. In other words, the Greedy scale (GS) was the highly abundant scale followed by the San Jose scale (SJS), but the olive scale represented the lowest one. The more or less increased and/or decreased abundance of each of the investigated scale insect species pointed to the existence of three overlapping generations throughout the season which were characterized by fluctuating peaks of population densities of the studied pests during the months of each annual season: autumn, winter, spring and summer.

  18. Brocklehurst's Textbook of Geriatric Medicine and Gerontology Fillit Howard M Rockwood Kenneth and Young John Brocklehurst's Textbook of Geriatric Medicine and Gerontology 1168pp £164.69 Elsevier 9780702061851 0702061859 [Formula: see text].

    PubMed

    2017-02-28

    This, the eighth edition of a well-regarded textbook, has been revised to focus on frailty. Its contributors include several nurses and the book covers a wide range of topics, including biological, medical, psychological and social gerontology. It also covers all the main body systems, which means it should be useful to nurses across a range of specialties.

  19. Brocklehurst's Textbook of Geriatric Medicine and Gerontology (8th edition) Fillit Howard M Rockwood Kenneth , and Young John Brocklehurst's Textbook of Geriatric Medicine and Gerontology (8th edition) 1168pp £182.99 Elsevier 9780702061851 0702061859 [Formula: see text].

    PubMed

    2016-12-07

    This is the 8th edition of this well-regarded medical textbook. Its contributors include several nurses and the subjects covered will be of interest to any nurse or nursing student working with older adults.

  20. Recognizing and honoring Howard University School of Law's 140-year legacy of social justice and its continued commitment to the training of capable and compassionate legal practitioners and scholars.

    THOMAS, 111th Congress

    Rep. Kilpatrick, Carolyn C. [D-MI-13

    2009-07-28

    10/22/2009 Referred to the Subcommittee on Higher Education, Lifelong Learning, and Competitiveness. (All Actions) Tracker: This bill has the status Passed HouseHere are the steps for Status of Legislation:

  1. How HANDy Are Baby Signs? A Systematic Review of the Impact of Gestural Communication on Typically Developing, Hearing Infants under the Age of 36 Months: Response to Howard and Doherty-Sneddon's Commentary

    ERIC Educational Resources Information Center

    Fitzpatrick, Elizabeth M.; Johnston, J. Cyne; Thibert, Jonelle; Grandpierre, Viviane

    2014-01-01

    A systematic review was conducted to synthesize the evidence related to the effectiveness of baby sign language for children with typical development. This response to a Commentary on the review stresses that the primary purpose of the review was to assist caregivers and policy makers with informed decision-making related to the benefits of the…

  2. 78 FR 36219 - Final Flood Hazard Determinations

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-06-17

    ..., Maryland Docket No.: FEMA-B-1236 Unincorporated Areas of Howard County.. Howard County Department of Public Works, Bureau of Environmental Services, 6751 Columbia Gateway Drive, Suite 514, Columbia, MD...

  3. 14. UNIDENTIFIED CABLESTAYED SUSPENSION BRIDGE WITH TIMBER RAILING OF TEN ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    14. UNIDENTIFIED CABLE-STAYED SUSPENSION BRIDGE WITH TIMBER RAILING OF TEN PANELS AND STONE PIER, SHOWING ELEMENTS SIMILAR TO E.E. RUNYON'S SUSPENSION BRIDGE PATENTS, BUT ALTERNATE CABLE PATTERN. 3/4 VIEW FROM BELOW. - Bluff Dale Suspension Bridge, Spanning Paluxy River at County Route 149, Bluff Dale, Erath County, TX

  4. Development of Cursor-on-Target Control for Semi-Autonomous Unmanned Aircraft Systems

    DTIC Science & Technology

    2007-06-01

    on what the brain perceives, psychologist are also studying “unconscious inference,” which are assumptions the brain believes ( Purves , 2007). This...19, Providence, Rhode Island, August 2004. Purves , Dale “The answer indicated by perceptual evidence,” Laboratory of Dale Purves , Center for

  5. 75 FR 145 - Clean Air Act Operating Permit Program; Petition for Objection to State Operating Permit for East...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-01-04

    ... Permit Program; Petition for Objection to State Operating Permit for East Kentucky Power Cooperative, Inc.-- William C. Dale Power Station; Clark County, KY AGENCY: Environmental Protection Agency (EPA). ACTION... for Air Quality (KDAQ) to East Kentucky Power Cooperative, Inc. (EKPC) for its William C. Dale...

  6. 15. View of the Main Office (Buildings 1, 2, and ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    15. View of the Main Office (Buildings 1, 2, and 3), U.S. Plant Introduction Station, Glenn Dale, MD, ca. 1950. Facilities and Engineering Branch. Beltsville Agricultural Research Center, Building 426. - U.S. Plant Introduction Station, 11601 Old Pond Road, Glenn Dale, Prince George's County, MD

  7. Design of a Prototype Microcomputer Driven Training Device for an Air Force Maintenance School

    DTIC Science & Technology

    1979-08-01

    digital system." 3. Kargo, Donald W. and Steffen, Dale A. Performance Training Carrel for Electronics Principles Course, Sept 76, AFHRL-TR 76-62(1), pg 1...June 1977 Kargo, Donald W. and Steffen, Dale A. Performance Training Corral for Electronics Principles Course, AFHRL-TR 76-62(i), Air Force Human

  8. The Effectiveness of the Smog Index in Determining the Reading Levels of Business and Distributive Education Texts.

    ERIC Educational Resources Information Center

    Schultheis, Robert A.; Anderson, Roberta

    1982-01-01

    McLaughlin's Smog Index was compared to the Dale-Chall formula for the determination of reading levels of 48 textbooks in business and distributive education. A Modified Smog Index proved a valid substitute for the Dale-Chall formula when used to evaluate the reading levels of business and distributive education narrative. (Author/CT)

  9. 78 FR 19708 - Farm Credit Administration Board; Sunshine Act; Regular Meeting

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-04-02

    ... a.m. until such time as the Board concludes its business. FOR FURTHER INFORMATION CONTACT: Dale L... Inflation--Final Rule. Quarterly Report on Farm Credit System Condition. FCS Building Association Auditor's..., 2013. Dale L. Aultman, Secretary, Farm Credit Administration Board. BILLING CODE 6705-01-P...

  10. Computational Study of Inlet Active Flow Control

    DTIC Science & Technology

    2007-05-01

    respectively. 3 Figure 2: Howard University Baseline Grid File created from Gridgen mesh Howard University also created a refined grid with... Gridgen mesh 4 Simulation Details and Results In total, three solutions were obtained from the study, one using the Howard University Baseline

  11. Mapping Me: Mapping Identity among Academically High-Performing Black Males

    ERIC Educational Resources Information Center

    Flennaugh, Terry K.

    2016-01-01

    Background: Scholars have argued that far too little research has examined the complex processes that many Black males undertake in constructing identities that function in schools (Howard & Flennaugh, 2011; Howard, Flennaugh, & Terry, 2012; Terry, Flennaugh, Blackmon, & Howard, 2014). Some have highlighted the perpetuation of a false…

  12. 40 CFR 81.316 - Iowa.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... Henry County Howard County Humboldt County Ida County Iowa County Jackson County Jasper County Jefferson... Henry County Howard County Humboldt County Ida County Iowa County Jackson County Jasper County Jefferson... Harrison County Henry County Howard County Humboldt County Ida County Iowa County Jackson County...

  13. 37 CFR 102.34 - Specific exemptions.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    .../DEPT-14; (D) Attorneys and Agents Registered to Practice Before the Office—COMMERCE/PAT-TM-1; (E... OF COMMERCE ADMINISTRATION DISCLOSURE OF GOVERNMENT INFORMATION Privacy Act § 102.34 Specific... are within this exemption are: COMMERCE/PAT-TM-6, COMMERCE/PAT-TM-7, COMMERCE/PAT-TM-8,...

  14. Fundamentals of nursing made incredibly easy! - first UK edition Pat Berridge Fundamentals of nursing made incredibly easy! - first UK edition Catherine Liddle Lippincott Williams & Wilkins 656pp £25.95 978 1 9018 3113 9 1901831132 [Formula: see text].

    PubMed

    2012-04-25

    This weighty American book has recently been adapted for the UK market. While a few Americanisms remain, nothing problematic has resulted from the adaptation, and the content is clear and straightforward.

  15. Multiscale Functional and Molecular Photoacoustic Tomography.

    PubMed

    Yao, Junjie; Xia, Jun; Wang, Lihong V

    2016-01-01

    Photoacoustic tomography (PAT) combines rich optical absorption contrast with the high spatial resolution of ultrasound at depths in tissue. The high scalability of PAT has enabled anatomical imaging of biological structures ranging from organelles to organs. The inherent functional and molecular imaging capabilities of PAT have further allowed it to measure important physiological parameters and track critical cellular activities. Integration of PAT with other imaging technologies provides complementary capabilities and can potentially accelerate the clinical translation of PAT.

  16. Multi-scale Functional and Molecular Photoacoustic Tomography

    PubMed Central

    Yao, Junjie; Xia, Jun; Wang, Lihong V.

    2015-01-01

    Photoacoustic tomography (PAT) combines rich optical absorption contrast with the high spatial resolution of ultrasound at depths in tissue. The high scalability of PAT has enabled anatomical imaging of biological structures ranging from organelles to organs. The inherent functional and molecular imaging capabilities of PAT have further allowed it to measure important physiological parameters and track critical cellular activities. Integration of PAT with other imaging technologies provides complementary capabilities and can potentially accelerate the clinical translation of PAT. PMID:25933617

  17. Problemes theoriques et methodologiques dans l'etude des langues/dialectes en contact aux niveaux macrologique et micrologique = Theoretical and Methodological Issues in the Study of Languages/Dialects in Contact at Macro- and Micro-Logical Levels of Analysis. Proceedings of the International Conference DALE (University of London)/ICRB (Laval University, Quebec)/ICSBT (Vrije Universiteit te Brussel) (London, England, May 23-26, 1985).

    ERIC Educational Resources Information Center

    Blanc, Michel, Ed.; Hamers, Josiane F., Ed.

    Papers from an international conference on the interaction of languages and dialects in contact are presented in this volume. Papers include: "Quelques reflexions sur la variation linguistique"; "The Investigation of 'Language Continuum' and 'Diglossia': A Macrological Case Study and Theoretical Model"; "A Survey of…

  18. PEDESTRIAN UNDERPASS AT CRESCENT ROAD AND ROOSEVELT CENTER FROM NORTH. ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    PEDESTRIAN UNDERPASS AT CRESCENT ROAD AND ROOSEVELT CENTER FROM NORTH. NOTE DALE WINLING, 2005 SALLY KRESS TOMPKINS FELLOW, WALKING THROUGH UNDERPASS. - Old Greenbelt, Crescent Road and Southway, Greenbelt, Prince George's County, MD

  19. Confrontational Teaching and Rhetorical Practice.

    ERIC Educational Resources Information Center

    Anderson, Virginia

    1997-01-01

    Argues that rhetorical theory enables a constructivist critique of activist pedagogy. Considers two prominent formulations of activist teaching--by Dale Bauer and James Berlin--examining both the underlying assumptions and descriptions of practice in rhetorical terms. (PA)

  20. 5. VIEW EAST, INTERIOR OF UNNUMBERED SCREENHOUSE, WITH SOUTH WALL ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    5. VIEW EAST, INTERIOR OF UNNUMBERED SCREENHOUSE, WITH SOUTH WALL OF HEADHOUSE (BUILDING 4) AT LEFT - U.S. Plant Introduction Station, Main Headhouse & Lean-to Greenhouses, 11601 Old Pond Road, Glenn Dale, Prince George's County, MD

  1. 2. VIEW SOUTHEAST, LEANTO GREENHOUSES OF MAIN HEADHOUSE (BUILDINGS 22. ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    2. VIEW SOUTHEAST, LEAN-TO GREENHOUSES OF MAIN HEADHOUSE (BUILDINGS 22. 23) - U.S. Plant Introduction Station, Main Headhouse & Lean-to Greenhouses, 11601 Old Pond Road, Glenn Dale, Prince George's County, MD

  2. VIEW NORTHEAST, WEST FRONT OF PACKING SHED (BUILDING 20) AND ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    VIEW NORTHEAST, WEST FRONT OF PACKING SHED (BUILDING 20) AND BAMBOO STAND ALONG SOUTH FRONT - U.S. Plant Introduction Station, Packing Shed, 11601 Old Pond Road, Glenn Dale, Prince George's County, MD

  3. Final Peer Review Report for IGEM and ADAGE

    EPA Pesticide Factsheets

    Final peer review report, which includes the following: IEc Peer Review Documentation Memorandum; Report of the Peer Review Panel; Responses to Panel prepared by Dale Jorgenson Associates and by Resource Triangle Institute.

  4. Global Maritime Awareness

    DTIC Science & Technology

    2009-06-01

    Dale.V.Ferriere@uscg.mil Outreach & Coordination Captain George.McCarthy@uscg.mil Information Technology Captain Rafael Nieves @uscg dhs mil . . . Science & Technology, Including Space Systems & NMATS Guy Thomas george.g.thomas@uscg.mil

  5. 51. LAUNCH CREW IN FRONT OF BARGE ON RAIL BUGGIES. ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    51. LAUNCH CREW IN FRONT OF BARGE ON RAIL BUGGIES. (LEFT OT RIGHT: DALE WILBURN, GENE EVERLY, TIM DOEPELHEUER, JACK ROBERTS, JAMES REARICK, STEVE RAKUSKIN) - Hillman Barge & Construction Company, Paul Thomas Boulevard, Brownsville, Fayette County, PA

  6. Open Rotor: New Option for Jet Engines

    NASA Video Gallery

    NASA's Dale Van Zante describes how the open rotor propulsion system will be tested in a wind tunnel at NASA's Glenn Research Center. Open rotor aircraft engines use high-speed propellers and are c...

  7. Syrians flee violence and disrupted health services to Jordan.

    PubMed

    Gavlak, Dale

    2013-06-01

    Half a million Syrian refugees are sheltering in Jordan. Dale Gavlak reports on how United Nations agencies are helping the government respond to their needs, especially those of mothers and young children.

  8. Lung transplant

    MedlinePlus

    ... in the arteries of the lungs ( pulmonary hypertension ) Sarcoidosis Lung transplant may not be done for people ... Chronic Cystic fibrosis Idiopathic pulmonary fibrosis Lung disease Sarcoidosis Review Date 4/13/2015 Updated by: Dale ...

  9. Travelers' Health: Pregnant Travelers

    MedlinePlus

    ... Disabilities Pregnant Travelers Diane F. Morof, I. Dale Carroll INTRODUCTION Pregnancy is an altered state of health ... Obstet Gynecol. 2009 Oct;114(4):954–5. Carroll ID, Williams DC. Pre-travel vaccination and medical ...

  10. 1. VIEW EAST, WEST FRONT OF SOIL CONSERVATION SERVICE CLUSTER ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    1. VIEW EAST, WEST FRONT OF SOIL CONSERVATION SERVICE CLUSTER (BUILDINGS 24, 25, 26) - U.S. Plant Introduction Station, Soil Conservation Service Cluster, 11601 Old Pond Road, Glenn Dale, Prince George's County, MD

  11. 2. VIEW SOUTHEAST, WEST FRONT OF BUILDINGS 25. 26; NORTH ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    2. VIEW SOUTHEAST, WEST FRONT OF BUILDINGS 25. 26; NORTH FRONT OF BUILDING 24: ROOF GABLE OF BUILDING 27 - U.S. Plant Introduction Station, Soil Conservation Service Cluster, 11601 Old Pond Road, Glenn Dale, Prince George's County, MD

  12. 3. VIEW NORTHEAST, SOUTH FRONT OF SOIL CONSERVATION SERVICE CLUSTER ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    3. VIEW NORTHEAST, SOUTH FRONT OF SOIL CONSERVATION SERVICE CLUSTER (BUILDING 25) - U.S. Plant Introduction Station, Soil Conservation Service Cluster, 11601 Old Pond Road, Glenn Dale, Prince George's County, MD

  13. Diet and eating after esophagectomy

    MedlinePlus

    ... how to care for the tube. This includes flushing the tube with water before and after feedings ... 3/8/2015 Updated by: Dale Mueller, MD, Cardiovascular and Thoracic Surgeon, HeartCare Midwest; Chairman Department of ...

  14. Is It True That Smoking Causes Wrinkles?

    MedlinePlus

    Healthy Lifestyle Quit smoking Is it true that smoking causes wrinkles? Answers from Lowell Dale, M.D. Yes. ... 10, 2014 Original article: http://www.mayoclinic.org/healthy-lifestyle/quit-smoking/expert-answers/smoking/faq-20058153 . Mayo ...

  15. 77 FR 35384 - Farm Credit System Insurance Corporation Board; Regular Meeting

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-06-13

    ... requiring assistance should make arrangements in advance. The matters to be considered at the meeting are... Assistance Mid-Year Review of Insurance Premium Rates Dated: June 7, 2012. Dale L. Aultman, Secretary,...

  16. 17. INTERIOR VIEW OF WEST TRUSS, SHOWING RAILING, SUSPENSION CABLE, ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    17. INTERIOR VIEW OF WEST TRUSS, SHOWING RAILING, SUSPENSION CABLE, CONNECTION BOLTS AND 'U'-COUPLINGS, LOOKING SOUTHWEST - San Rafael Bridge, Spanning San Rafael River near Buckhorn Wash, Castle Dale, Emery County, UT

  17. Noninvasive Assessment of the Effect of Position and Exercise on Pulse Arrival to Peripheral Vascular Beds in Healthy Volunteers

    PubMed Central

    Obata, Yurie; Ong, Qi J.; Magruder, J. T.; Grichkevitch, Helen; Berkowitz, Dan E.; Nyhan, Daniel; Steppan, Jochen; Barodka, Viachaslau

    2017-01-01

    Background: The effects of position and exercise on pulse wave distribution across a healthy, compliant arterial tree are not fully understood. We studied the effects of exercise and position on the pattern of pulse arrival times (PATs) in healthy volunteers. Moreover, we compared the pulse arrival time ratios to the respective distance ratios between different locations. Methods: Thirteen young healthy volunteers were studied, using an electrocardiogram and plethysmograph to simultaneously record pulse wave arrival at the ear lobe, index finger and big toe. We compared the differences in PAT between each location at rest and post-exercise in the supine, sitting, and standing position. We also compared the PAT ratio (toe/ear, toe/finger, and finger/ear) to the corresponding pulse path distance ratios. Results: PAT was shortest at the ear then finger and longest at the toe regardless of position or exercise status. PATs were shorter post-exercise compared to rest. When transitioning from a standing to sitting or supine position, PAT to the ear decreased, while the PAT to the toe increased, and PAT to the finger didn't significantly change. PAT ratios were significantly smaller than predicted by the path distance ratios regardless of position or exercise status. Conclusions: Exercise makes PATs shorter. Standing position decrease PAT to the toe and increase to the ear. We conclude that PAT and PAT ratio represent the arterial vascular tree properties as surely as pulse transit time and pulse wave velocity. PMID:28220077

  18. TA53:19 Reverb Chamber Quick-Look

    SciTech Connect

    Bishofberger, Kip A.

    2016-05-19

    Within Building 19 of TA-53, a screen room has been evaluated for use as a reverb chamber (with deep gratitude to Dale Dalmas and Greg Dale for their assistance). With minimal additional sealing of the chamber, we expect the Q to increase even more, and thus field levels for the same RF source power. Future studies need to determine leakage field levels, which will define maximum achievable field levels.

  19. Analysis and Modeling of Target Echo and Reverberation: FY15 Annual Report for ONR

    DTIC Science & Technology

    2015-09-30

    during the past year: • Michael A. Ainslie, Dale D. Ellis, and Chris H. Harrison . Low frequency bottom reverberation in a Pekeris waveguide with...Chris H. Harrison . Towards benchmarks of low frequency reverberation level in a Pekeris waveguide: Insight from analytical solutions. J. Acoust...USA, 23–27 May 2011. [AEH15] Michael A. Ainslie, Dale D. Ellis, and Chris H. Harrison . Low frequency bottom reverberation in a Pekeris waveguide

  20. The Experience of Strategic Thinking in a Volatile, Complex, Uncertain & Ambiguous (VUCA) Environment

    DTIC Science & Technology

    2014-08-01

    Dale L. Moore, Aug., 2014 The Experience of Strategic Thinking in a Volatile, Complex, Uncertain & Ambiguous (VUCA) Environment Dr. Dale L...TITLE AND SUBTITLE The Experience of Strategic Thinking in a Volatile, Complex, Uncertain & Ambiguous (VUCA) Environment 5a. CONTRACT NUMBER 5b...Title: The Experience of Strategic Thinking in VUCA Environments Purpose of the Study: To understand the experience of leaders when they think

  1. An Evaluation of Alternative Resolution Procedures for Jurisdictional Disputes in the Construction Industry

    DTIC Science & Technology

    1983-03-01

    very existence. Mr. Dale Gemmill of the Keystone Building Contractors Association suggests that "if unions don’t put their house in order, the...Laborers Local 1184 (H. M. Robeztson Pipeline Constructors), 192 NLRB 1078 (1971) 40/ Ironworkers Local 383 (J. P. Cullen & Sons), 235 NLRB 463 1978 ±1...The inclosed questionnaire is designed to supplement my personal interviews with various contractor(ie Dale Cemmill, Keystone Builders Association

  2. Interagency in the Combatant Command: A Model for Efficiency Now Without Waiting for Legislation

    DTIC Science & Technology

    2014-05-15

    Presidential Directive, the CORDS program ensured the US unity of effort in Vietnam 11 Dale Andrade and...LCOL James Willbanks, “CORDS/Phoenix: Counterinsurgency Lessons from Vietnam for the Future,” Military Review (2006): 9-23. 12 Dale Andrade , “Three...to the post of civilian deputy 15 Andrade and Willbanks, “CORDS/Phoenix,” 14. 16 Mandy Honn et al

  3. Field Test of the Unit Equal Opportunity Training Diagnosis and Assessment System (TDAS)

    DTIC Science & Technology

    1980-02-01

    Reseiirch Note 80-38 In U FIELD TEST OF THE UNIT EQUAL OPPORTUNITY TRAINING DIAGNOSIS AND ASSESSMENT SYSTEM (TDAS) 0 Dale K. Brown Human Sciences...likely to be a unit problem area to that least likely. Four attitude scales are also included, as is a measure of knowledge of Army equal opportunity...UNIT EQUAL OPPORTUNITY TRAINING DIAGNOSIS AND ASSESSMENT SYSTEM Dale K. Brown Human Sciences Research, Inc. S James A. Thomas Team Chief ARI FIELD UNIT

  4. Modeling and Analysis of Target Echo and Clutter in Range-Dependent Bistatic Environments: FY13 Annual Report for ONR

    DTIC Science & Technology

    2013-09-30

    13] Michael A. Ainslie, Dale D. Ellis, Jan Ehrlich , Pieter Schippers, Karl-Thomas Hjelmervik, and Mathieu E. G. D. Colin. Echo, reverberation and echo...gebco 08.nc, version 20100927. [HOSH10] Paul C. Hines, John C. Osler, Jeffrey G. E. Scrutton, and Landon J. S. Halloran. Time-of-flight measurements...Acoustics held in Edinburgh, Scotland, UK, 2–6 July 2012. [published] • Michael A. Ainslie, Dale D. Ellis, Jan Ehrlich , Pieter Schippers, Karl-Thomas

  5. Intrepidity, Iron Will, and Intellect: General Robert L. Eichelberger and Military Genius

    DTIC Science & Technology

    2007-11-02

    topically listing references to such terms as military genius and coup d’oeil. Author Michael Howard argues in the preface to A Short Guide to Clausewitz On...falls far short of the intended goal” (Clausewitz 1989, 119). As author Michael Howard stated, Clausewitz believed that the commander’s determination...motion” ( Howard 1967, x). Clausewitz also discussed subcomponents or qualities of determination. Energy was a contagious enthusiasm that could motivate

  6. Navy/ASEE (American Society for Engineering Education) Summer Faculty Research Program, 1985.

    DTIC Science & Technology

    1986-05-15

    AUGUSTUS GERALD CHACHERE MANHATTAN COLLEGE-RIVERDALE HOWARD UNIVERSITY MECHANICAL ENGINEERING MATHEMATICS MECHANICAL ENGINEERING DEPT. 2441 6TH STREET, NW...PHYSICS 190 W. 19TH AVENUE DEPT. OF MATHEMATICS/PHYSICS COLUMBUS ,OH 43210 RENSSELAER ,IN 47978 CAROLYN F. BAILEY RICHARD V. CONTE HOWARD UNIVERSITY...DEPARTMENT MECHANICAL ENGR’G DEPT. SAN FRANCISCO CA 94132 WASHINGTON ,DC 20046 I ,II ATTACHMENT F. TEPPER L. ]ILL AYLE JCY HOWARD ;NI7ERI ST. UN:V. OF

  7. Sub-Saharan Africa Report

    DTIC Science & Technology

    1987-02-19

    quotes Trevor Howard, a former member of the 23d Battalion, an elite unit led by South African officers and white mercenaries recruited by the puppet...intervene only to raise their flag," claims Trevor Howard. AFRICA CONCORD says that it is crucial for South Africa to maintain the UNITA terrorist...racist army had.to mobilize its best land and air facilities to preclude the seizure of Mavinga by the Angolan Armed Forces. Trevor Howard documented the

  8. Impact of Erb-B Signaling on Myelin Repair in the CNS Following Virus-Induced Damage

    DTIC Science & Technology

    2008-03-01

    demyelinating disease. Microb Pathog 3, 319-326 (1987) 11. Richard J. Clatch, Stephen D. Miller, Roland Metzner, Mauro C. Dal Canto, and Howard L. Lipton...176, 244-254 (1990) 12. Mauro C. Dal Canto and Howard L. Lipton: Ultrastructural immunohistochemical localization of virus in acute and chronic...initiates in the CNS in two mouse models of multiple sclerosis. Nat Med 11, 335-339 (2005) 18. Mauro C. Dal Canto and Howard L. Lipton: Primary

  9. 76 FR 66718 - Clean Air Act Advisory Committee (CAAAC): Notice of Meeting

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-10-27

    ..., please contact Pat Childers, Office of Air and Radiation, U.S. EPA (202) 564-1082, FAX (202) 564-1352 or...-1082 or childers.pat@epa.gov . To request accommodation of a disability, please contact Mr....

  10. 75 FR 9426 - Maryland; Major Disaster and Related Determinations

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-03-02

    ... Baltimore, Caroline, Cecil, Harford, Howard, Kent, Montgomery, and Queen Anne's for emergency protective measures, (Category B) under the Public Assistance program. The counties of Baltimore, Caroline,...

  11. 4. Elevation, looking southwest, with Machine Shop and Goliath traveling ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    4. Elevation, looking southwest, with Machine Shop and Goliath traveling crane in background. - General Dynamics Corporation Shipyard, American Revolver Crane, 97 East Howard Street, Quincy, Norfolk County, MA

  12. Transgenic cry1C(⁎) gene rough rice line T1C-19 does not change the host preferences of the non-target stored product pest, Rhyzopertha dominica (Fabricius) (Coleoptera: Bostrichidae), and its parasitoid wasp, Anisopteromalus calandrae (Howard) (Hymenoptera: Pteromalidae).

    PubMed

    Sun, Xiao; Yan, Miao-Jun; Zhang, Aijun; Wang, Man-Qun

    2015-10-01

    Rough rice grains are often stored for extended periods before they are used or consumed. However, during storage, the rough rice is vulnerable to insect infestation, resulting in significant economic loss. Previous studies have shown that volatiles cues, physical characteristics, and taste chemicals on the grains could be the important key behavior factors for storage insect pests to locate the hosts and select oviposition sites. It is also well known that the transgenic Bt rough rice line T1C-19, which expresses a cry1C(⁎) gene has a high resistance to Lepidoptera pests. However, there were no evidences to show the consequences of host preference for non-target insect pests after growing Bt transgenic rice. In this study, the potential key factors of Bt rough rice were investigated for their impacts on the behaviors of non-target pest lesser grain borer Rhyzopertha dominica, the main weevil pest of grain and its parasitic wasps Anisopteromalus calandrae, the natural enemy of the beetle. Both electronic nose and electronic tongue analyses showed that the parameters of Bt rough rice were analogous to those of the non-Bt rough rice. The volatile profiles of Bt and non-Bt rough rice examined by gas chromatographic mass spectrometry (GC-MS) were similar. For most volatile compounds, there were no significantly quantitative differences in compound quantities between Bt and non-Bt rough rice. The densities of sclereids and trichomes on the rough rice husk surface were statistically equal in Bt and non-Bt rough rice. The non-target pest, R. dominica, and its parasitoid wasp, A. calandrae, were attracted to both rough rice and could not distinguish the transgenic T1C-19 from the isogenic rough rice. These results demonstrated that Bt rough rice has no negative impacts on the host preference behaviors of non-target stored product pest R. dominica and its parasitoid A. calandrae.

  13. Targeting Palmitoyl Acyltransferases in Mutant NRAS-Driven Melanoma

    DTIC Science & Technology

    2014-08-01

    to ~20% of melanoma with no effective treatment. Targeting palmitoyl acyltansferases (PATs) involved in N-RAS regulation could be a novel strategy to...treat N-RAS mutant melanoma . The objective of the project is to identify PATs responsible for NRAS activation in melanoma cells using chemical...have carried out PATs profiling in melanoma cells using chemical probes and mRNA profiling. We have identified candidate PATs highly expressed in NRAS

  14. Tutorial on photoacoustic tomography

    NASA Astrophysics Data System (ADS)

    Zhou, Yong; Yao, Junjie; Wang, Lihong V.

    2016-06-01

    Photoacoustic tomography (PAT) has become one of the fastest growing fields in biomedical optics. Unlike pure optical imaging, such as confocal microscopy and two-photon microscopy, PAT employs acoustic detection to image optical absorption contrast with high-resolution deep into scattering tissue. So far, PAT has been widely used for multiscale anatomical, functional, and molecular imaging of biological tissues. We focus on PAT's basic principles, major implementations, imaging contrasts, and recent applications.

  15. Enhanced Preliminary Assessment Report: Presidio of San Francisco Military Reservation, San Francisco, California

    DTIC Science & Technology

    1989-11-01

    HAZARD --------------------- ----------- -------- ------ -X-RAY (217) RD XRY ACETIC ACID VET LAB RV BID BORIC ACID VET LAB RV BID SODIUM DICHROMATE...GHC HYDROGEN PEROXIDE (90%) 202 HL PAT NITROGEN OXIDES 212 HL PAT BORIC ACID 202 HL PAT ETHYLENE GLYCOL, VAPOR 202 HL PAT XYLENE(O-,M-,P-ISOMERS) 202...10. Ammonium Oxalate 11. Ammonium Sulfate 12. Alphanaphthol 13. L-Ascorbic Acid 14. Barbital - toxic 15. Barium Chloride - flammable 16. Boric Acid 17

  16. Partnership for a Drug-Free America: Partnership Attitude Tracking Study. Teens: Ethnic and Racial Trends, Spring 2002.

    ERIC Educational Resources Information Center

    Delaney, Barbara

    The annual Partnership Attitude Tracking Study (PATS) tracks consumers' attitudes about illegal drugs. PATS consists of two nationally projectable samples: a teen sample for students in grades 7-12 and a parent sample. The 2002 PATS, conducted in homes and schools, collected data using self-report surveys. Results indicate that after a decade of…

  17. Persuasive Arguments Theory: A Test of Assumptions.

    ERIC Educational Resources Information Center

    Meyers, Renee A.

    1989-01-01

    Assesses Persuasive Arguments Theory (PAT) assumptions about argument and argument influence. Reviews and critiques PAT, advancing five hypotheses to test its assumptions. Finds little correspondence between cognitive and discussion arguments in number, content, or persuasiveness. Discusses implications for PAT and for the role of communication in…

  18. Travel and Correspondence

    ERIC Educational Resources Information Center

    Shauck, Barry

    2004-01-01

    Professional development through the advanced planning of themed exhibitions provides art instructors in Howard County the impetus for many art problems. This art problem, centered on the art and writing contained in the storybooks of Holly Hobbie. In late summer, the art teaching staff of the Howard County Public School System gathered at the…

  19. All That Remains: The Traditional Architecture and Engineering Structures of the Richard B. Russel Multiple Resource Area, Georgia and South Carolina. Appendix B.

    DTIC Science & Technology

    1983-01-01

    BERRY ( HOWARD UNIVERSITY), DAVID T. MARSH JR. ( HOWARD UNIVERSITY), AND STAFF LANDSCAPE ARCHITECT, PAUL D. DOLUNSKY. GROGAN HOUSE IEUREKA I 500 300...VAS EXECUTED UNDER THE DIRECTION OF ROBNERT ICAPUHCHU 4E OF NAER; JOHN V OF GEORGI ). THE HAS FIELD OFFICE, ELBERTON, GEORGA. ,,TEA PP IRCHE FHBiN

  20. 75 FR 31413 - Request for Extension and Revision of a Currently Approved Information Collection

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-06-03

    ... following methods: By Mail to: David Wiggins or Jay Howard-Brock, Civil Rights and Community Outreach, USDA.... FOR FURTHER INFORMATION CONTACT: Contact David Wiggins or Jay Howard- Brock, Civil Rights and... Corporation administers cooperative agreements that will be used to provide outreach and assistance to...

  1. 40 CFR 81.326 - Missouri.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... Daviess County De Kalb County Gentry County Grundy County Harrison County Holt County Howard County Knox... County Cooper County Daviess County DeKalb County Gentry County Grundy County Harrison County Holt County... County Grundy County Harrison County Holt County Howard County Knox County Lewis County Lincoln...

  2. Center-of-Gravity Analysis in COIN: A New Way to Problem-Solve

    DTIC Science & Technology

    2009-03-20

    Howard and Peter Paret (Oxford: Oxford University Press , 2007), 242-42. 2 Headquarters, Department of the Army, Field Manual 3-24, Counterinsurgency...Clausewitz, On War, Abridged, Annotated. Translated by Michael Howard and Peter Paret. Oxford: Oxford University Press , 2007. Colonel Peter R

  3. Aussie Battler, or Worldly Opportunist?

    ERIC Educational Resources Information Center

    Walter, James

    2004-01-01

    In this article, the author comments on Judith Brett's article (see EJ848172). Brett dissects the differences between the Howard world-view and that of the intelligentsia, using Robert Merton's dichotomy between "locals" and "cosmopolitans" as her means. On this account, Howard's preoccupation with national sovereignty--and…

  4. What Helps Us Learn?

    ERIC Educational Resources Information Center

    Educational Leadership, 2010

    2010-01-01

    This article presents comments of high school students at the Howard Gardner School in Alexandria, Virginia, who were asked, What should teachers know about students to help them learn? Twelve high school students from the Howard Gardner School in Alexandria, Virginia, describe how their best teachers get to know them and thus were more able to…

  5. Changing Views of Culture and Behavior: An Interview with Robert Serpell

    ERIC Educational Resources Information Center

    Lockett, Charles T.

    2003-01-01

    Charles Lockett is an Assistant Professor in the School of Psychology at James Madison University, where he teaches developmental psychology as well as advanced topic courses in cultural psychology and the psychology of race and racism. A graduate of Howard University, Lockett credits Howard's Preparing Future Faculty Fellowship Program for his…

  6. 40 CFR 81.316 - Iowa.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... Henry County Howard County Humboldt County Ida County Iowa County Jackson County Jasper County Jefferson... Henry County Howard County Humboldt County Ida County Iowa County Jackson County Jasper County Jefferson.... Jasper County Unclassifiable/Attainment. Jefferson County Unclassifiable/Attainment. Johnson...

  7. 40 CFR 81.316 - Iowa.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... Henry County Howard County Humboldt County Ida County Iowa County Jackson County Jasper County Jefferson... Henry County Howard County Humboldt County Ida County Iowa County Jackson County Jasper County Jefferson.... Jasper County Unclassifiable/Attainment. Jefferson County Unclassifiable/Attainment. Johnson...

  8. Leadership under the Golden Rule

    ERIC Educational Resources Information Center

    Hebel, Sara

    2009-01-01

    The author interviews Muriel A. Howard, president of the State University of New York College at Buffalo, who is set to become the first black leader of any of the "big six" higher-education associations in Washington. She was named last week as the new president of the American Association of State Colleges and Universities. Ms. Howard,…

  9. Coalition Logistics: The Way to Win the Peace, The Way to Win the War

    DTIC Science & Technology

    2007-06-05

    Policy for the Coming Decade,” 51-56; and Howard Zinn , “The Power and the Glory: Myths of American Exceptionalism,” Boston Review; available from http...31how cos.htm. Internet. Accessed on 12 January 2007. Zinn , Howard . “The Power and the Glory: Myths of American Exceptionalism.” Boston Review

  10. American Exceptionalism and a New World Order

    DTIC Science & Technology

    2010-02-01

    Gutek, Philosophical and Ideological Voices in Education, 3rd ed. (Boston: Pearson Education, 2004). 2 Howard Zinn , The Power and the Glory, Summer...and U.S. Foreign Policy (London: lynne Reinner Publishers, 2002). 7 Howard Zinn , The Power and the Glory, Summer 2005, http://bostonreview.net

  11. 75 FR 71525 - Prevailing Rate Systems; Redefinition of the Chicago, IL; Fort Wayne-Marion, IN; Indianapolis, IN...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-11-24

    ... Indianapolis wage area; Carroll and Howard Counties, IN, from the Fort Wayne-Marion wage area to the Indianapolis wage area; and Carroll County, OH, from the Pittsburgh wage area to the Cleveland wage area. These..., wage area; Carroll and Howard Counties, IN, from the Fort Wayne-Marion, IN, wage area to...

  12. 75 FR 39460 - Prevailing Rate Systems; Redefinition of the Chicago, IL; Fort Wayne-Marion, IN; Indianapolis, IN...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-07-09

    ... would redefine Benton County, IN, from the Chicago wage area to the Indianapolis wage area; Carroll and Howard Counties, IN, from the Fort Wayne-Marion wage area to the Indianapolis wage area; and Carroll...; Carroll and Howard Counties, IN, from the Fort Wayne-Marion wage area to the Indianapolis wage area;...

  13. False Assumptions: Military Assistance Command Vietnam s (MACV) use of the Combined Strategic Objectives Plan, 1970 and its Operationalization at the Field Force Level

    DTIC Science & Technology

    2014-12-04

    University Press, 2011), 91. 25 Mike Gravel, Noam Chomsky , Howard Zin, The Pentagon Papers: The Senator Gravel Edition (Boston: Beacon Press, 1971), 606...York: Atlantic Monthy Press, 1986. 55 Gravel, Mike, Noam Chomsky , Howard Zinn. The Pentagon Papers: The Senator Gravel Edition. Boston: Beacon

  14. A World without Walls. Dimension: Languages 71. Proceedings of the Southern Conference on Language Teaching (7th, Atlanta, Georgia, October 1971).

    ERIC Educational Resources Information Center

    Wilkins, George W., Jr., Ed.

    Presentations on aspects of foreign language instruction include: "A World Without Walls" (Lorraine A. Strasheim); "Practical Aspects of Individualizing Foreign Language Instruction" (Howard B. Altman); "Teaching Foreign Language Through Literature" (Jeanne Varney Pleasants); "Individualization of Foreign Language Instruction" (Howard B. Altman);…

  15. The Shaping of NCEA 11

    ERIC Educational Resources Information Center

    Horrigan, Donald C.

    1977-01-01

    The preceeding article (EJ 163 640) in this series sketched the history of the NCEA through contributions made by two early leaders, Thomas J. Conaty and Francis W. Howard, 1899-1920. This article deals with the next twenty-five year period, 1920-1944, in the NCEA's history and the influence that Francis Howard and George Johnson had in helping…

  16. Mordecai Johnson: An Early Pillar of Black Higher Education.

    ERIC Educational Resources Information Center

    McKinney, Richard I.

    2000-01-01

    Discusses Mordecai Johnson, who became Howard University's first African American president and transformed Howard into the leading center for black higher education, where students could acquire knowledge, skills, and inspiration to prepare them to make a difference in the world. During his tenure, faculty and student numbers tripled. His major…

  17. The Role of Home-Visiting Programs in Preventing Child Abuse and Neglect

    ERIC Educational Resources Information Center

    Howard, Kimberly S.; Brooks-Gunn, Jeanne

    2009-01-01

    Kimberly Howard and Jeanne Brooks-Gunn examine home visiting, an increasingly popular method for delivering services for families, as a strategy for preventing child abuse and neglect. They focus on early interventions because infants are at greater risk for child abuse and neglect than are older children. In their article, Howard and Brooks-Gunn…

  18. The Art of Math

    ERIC Educational Resources Information Center

    Kim, Una; Stabley, Angela

    2010-01-01

    In the movie "A Beautiful Mind" there is a scene where future Nobel prize winner John Nash is captivated by a fellow student's tie. When asked about his behavior Nash quips, "There has to be a mathematical explanation for how bad that tie is." (Grazer & Howard, 2000) In this light moment, director Ron Howard attempts to show us that the mind of a…

  19. 50 CFR 217.85 - Requirements for monitoring and reporting.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ...-8401 and/or by email to Jolie.Harrison@noaa.gov and Howard.Goldstein@noaa.gov, and the NMFS Southeast Regional Marine Mammal Stranding Network at 877-433-8299 (Blair.Mase@noaa.gov and Erin.Fougeres@noaa.gov... to Jolie.Harrison@noaa.gov and Howard.Goldstein@noaa.gov and the NMFS Southeast Region Marine...

  20. Use of food and space by tunneler dung beetles (Coleoptera; Scarabaeinae) during reproduction.

    PubMed

    Huerta, Carmen; Anduaga, Sofía; López-Portillo, Jorge; Halffter, Gonzalo

    2010-08-01

    At two temperate pasturelands in northern Mexico, we explored possible competition for food and space under pats during the simultaneous nesting periods of the univoltine species Dichotomius colonicus (Say), Phanaeus quadridens (Say), and Copris sierrensis Matthews. To simulate unlimited resources, 50 5-kg cow dung pats were placed at regular distance intervals in each pastureland. After building trenches around the pats, the number and depth of each nest, as well as larval development status, were documented once for a period of 1-8 mo. Analyses of variance and association tests were used to make a between-site comparison of dung pat occupation, nests occupied per species, nests per dung pat, and nest depth below each pat. The proportion of pats occupied by each species differed significantly between sites. C. sierrensis colonizing most pats at one site and D. colonicus at the other. There were no differences between sites in the frequency of pats occupied by more than one species. The association test and Ochiai index showed that each species colonized dung pats independently. The results suggest that pat occupation depended on their location by beetles and the relative abundance of each species. The species tended to dig nests at different depths, possibly reducing interspecific competition for space. It can therefore be concluded that, when food resources seem to be unlimited, they are shared following a "lottery dynamic" model if there is spatial differentiation among species.