Sample records for hplc system equipped

  1. Current HPLC Methods for Assay of Nano Drug Delivery Systems.

    PubMed

    Tekkeli, Serife Evrim Kepekci; Kiziltas, Mustafa Volkan

    2017-01-01

    In nano drug formulations the mechanism of release is a critical process to recognize controlled and targeted drug delivery systems. In order to gain high bioavailability and specificity from the drug to reach its therapeutic goal, the active substance must be loaded into the nanoparticles efficiently. Therefore, the amount in biological fluids or tissues and the remaining amount in nano carriers are very important parameters to understand the potential of the nano drug delivery systems. For this aim, suitable and validated quantitation methods are required to determine released drug concentrations from nano pharmaceutical formulations. HPLC (High Performance Liquid Chromatography) is one of the most common techniques used for determination of released drug content out of nano drug formulations, in different physical conditions, over different periods of time. Since there are many types of HPLC methods depending on detector and column types, it is a challenge for the researchers to choose a suitable method that is simple, fast and validated HPLC techniques for their nano drug delivery systems. This review's goal is to compare HPLC methods that are currently used in different nano drug delivery systems in order to provide detailed and useful information for researchers. Copyright© Bentham Science Publishers; For any queries, please email at epub@benthamscience.org.

  2. HPLC separation of triacylglycerol positional isomers on a polymeric ODS column.

    PubMed

    Kuroda, Ikuma; Nagai, Toshiharu; Mizobe, Hoyo; Yoshimura, Nobuhito; Gotoh, Naohiro; Wada, Shun

    2008-07-01

    A polymeric ODS column was applied to the resolution of triacylglycerol positional isomers (TAG-PI), i.e. 1,3-dioleoyl-2-palmitoyl-glycerol (OPO) and 1,2-dioleoyl-3-palmitoyl-rac-glycerol (OOP), with a recycle HPLC system. To investigate the ODS column species and the column temperatures for the resolution of a TAG-PI pair, a mixture of OPO and OOP was subjected to an HPLC system equipped with a non-endcapped polymeric, endcapped monomeric, endcapped intermediate, or non-endcapped monomeric ODS column at three different column temperatures (40, 25, or 10 degrees C). Only the non-endcapped polymeric ODS column achieved the separation of OPO and OOP, and the lowest column temperature (10 degrees C) showed the best resolution for them. The other pair of TAG-PI, a mixture of 1,3-dipalmitoyl-2-oleoyl-glycerol (POP) and 1,2-dipalmitoyl-3-oleoyl-rac-glycerol (PPO) was also subjected to the system equipped with a non-endcapped polymeric or monomeric ODS column at five different column temperatures (40, 32, 25, 17, and 10 degrees C). Thus, POP and PPO were also separated on only the non-endcapped polymeric ODS column at 25 degrees C. However, no clear peak appeared at 10 degrees C. These results would indicate that the polymeric ODS stationary phase has an ability to recognize the structural differences between TAG-PI pairs. Also, the column temperature is a very important factor for separating the TAG-PI pair, and the optimal temperature would relate to the solubility of TAG-PI in the mobile phase. Furthermore, the recycle HPLC system provided measurements for the separation and analysis of TAG-PI pairs.

  3. Identification of phenanthrene derivatives in Aerides rosea (Orchidaceae) using the combined systems HPLC-ESI-HRMS/MS and HPLC-DAD-MS-SPE-UV-NMR.

    PubMed

    Cakova, Veronika; Urbain, Aurélie; Antheaume, Cyril; Rimlinger, Nicole; Wehrung, Patrick; Bonté, Frédéric; Lobstein, Annelise

    2015-01-01

    In our continued efforts to contribute to the general knowledge on the chemical diversity of orchids, we have decided to focus our investigations on the Aeridinae subtribe. Following our previous phytochemical study of Vanda coerulea, which has led to the identification of phenanthrene derivatives, a closely related species, Aerides rosea Lodd. ex Lindl. & Paxton, was chosen for investigation. To identify new secondary metabolites, and to avoid isolation of those already known, by means of the combined systems HPLC-DAD(diode-array detector) with high-resolution tandem mass spectrometry (HRMS/MS) and HPLC-DAD-MS-SPE(solid-phase extraction)-UV-NMR. A dereplication strategy was developed using a HPLC-DAD-HRMS/MS targeted method and applied to fractions from A. rosea stem extract. Characterisation of unknown minor compounds was then performed using the combined HPLC-DAD-MS-SPE-UV-NMR system. The dereplication method allowed the characterisation of four compounds (gigantol, imbricatin, methoxycoelonin and coelonin), previously isolated from Vanda coerulea stem extract. The analyses of two fractions permitted the identification of five additional minor constituents including one phenanthropyran, two phenanthrene and two dihydrophenanthrene derivatives. The full set of NMR data of each compound was obtained from microgram quantities. Nine secondary metabolites were characterised in A. rosea stems, utilising HPLC systems combined with high-resolution analytical systems. Two of them are newly described phenanthrene derivatives: aerosanthrene (5-methoxyphenanthrene-2,3,7-triol) and aerosin (3-methoxy-9,10-dihydro-2,5,7-phenanthrenetriol). Copyright © 2014 John Wiley & Sons, Ltd.

  4. Validation of an HPLC-UV method for the determination of ceftriaxone sodium residues on stainless steel surface of pharmaceutical manufacturing equipments.

    PubMed

    Akl, Magda A; Ahmed, Mona A; Ramadan, Ahmed

    2011-05-15

    In pharmaceutical industry, an important step consists in the removal of possible drug residues from the involved equipments and areas. The cleaning procedures must be validated and methods to determine trace amounts of drugs have, therefore, to be considered with special attention. An HPLC-UV method for the determination of ceftriaxone sodium residues on stainless steel surface was developed and validated in order to control a cleaning procedure. Cotton swabs, moistened with extraction solution (50% water and 50% mobile phase), were used to remove any residues of drugs from stainless steel surfaces, and give recoveries of 91.12, 93.8 and 98.7% for three concentration levels. The precision of the results, reported as the relative standard deviation (RSD), were below 1.5%. The method was validated over a concentration range of 1.15-6.92 μg ml(-1). Low quantities of drug residues were determined by HPLC-UV using a Hypersil ODS 5 μm (250×4.6 mm) at 50 °C with an acetonitrile:water:pH 7:pH 5 (39-55-5.5-0.5) mobile phase at flow rate of 1.5 ml min(-1), an injection volume of 20 μl and were detected at 254 nm. A simple, selective and sensitive HPLC-UV assay for the determination of ceftriaxone sodium residues on stainless steel surfaces was developed, validated and applied. Copyright © 2011 Elsevier B.V. All rights reserved.

  5. Information technology equipment cooling system

    DOEpatents

    Schultz, Mark D.

    2014-06-10

    According to one embodiment, a system for removing heat from a rack of information technology equipment may include a sidecar indoor air to liquid heat exchanger that cools warm air generated by the rack of information technology equipment. The system may also include a liquid to liquid heat exchanger and an outdoor heat exchanger. The system may further include configurable pathways to connect and control fluid flow through the sidecar heat exchanger, the liquid to liquid heat exchanger, the rack of information technology equipment, and the outdoor heat exchanger based upon ambient temperature and/or ambient humidity to remove heat from the rack of information technology equipment.

  6. Power Product Equipment Technician: Equipment Systems. Teacher Edition. Student Edition.

    ERIC Educational Resources Information Center

    Hilley, Robert

    This packet contains teacher and student editions on the topic of equipment systems, intended for the preparation of power product equipment technicians. This publication contains seven units: (1) principles of power transmission; (2) mechanical drive systems; (3) principles of fluid power; (4) hydraulic and pneumatic drive systems; (5) wheel and…

  7. The first use of a HPLC system at a Louisiana Sugarcane Factory: What it can do for you

    USDA-ARS?s Scientific Manuscript database

    Alma Plantation sugarcane factory established and operated the first High Performance Liquid Chromatography (HPLC) system in Louisiana in 2015. Although many HPLC systems exist, the factory opted for a ThermoFisherTM ion chromatography (anion exchange) system with integrated pulsed amperometric det...

  8. Comparison of HPLC & spectrophotometric methods for estimation of antiretroviral drug content in pharmaceutical products.

    PubMed

    Hemanth Kumar, A K; Sudha, V; Swaminathan, Soumya; Ramachandran, Geetha

    2010-10-01

    Simple and reliable methods to estimate drugs in pharmaceutical products are needed. In most cases, antiretroviral drug estimations are performed using a HPLC method, requiring expensive equipment and trained technicians. A relatively simple and accurate method to estimate antiretroviral drugs in pharmaceutical preparations is by spectrophotometric method, which is cheap and simple to use as compared to HPLC. We undertook this study to standardise methods for estimation of nevirapine (NVP), lamivudine (3TC) and stavudine (d4T) in single tablets/capsules by HPLC and spectrophotometry and to compare the content of these drugs determined by both these methods. Twenty tablets/capsules of NVP, 3TC and d4T each were analysed for their drug content by HPLC and spectrophotometric methods. Suitably diluted drug solutions were run on HPLC fitted with a C18 column using UV detection at ambient temperature. The absorbance of the diluted drug solutions were read in a spectrophotometer at 300, 285 and 270 nm for NVP, 3TC and d4T respectively. Pure powders of the drugs were used to prepare calibration standards of known drug concentrations, which was set up with each assay. The inter-day variation (%) of standards for NVP, 3TC and d4T ranged from 2.5 to 6.7, 2.1 to 7.7 and 6.2 to 7.7, respectively by HPLC. The corresponding values by spectrophotometric method were 2.7 to 4.7, 4.2 to 7.2 and 3.8 to 6.0. The per cent variation between the HPLC and spectrophotometric methods ranged from 0.45 to 4.49 per cent, 0 to 4.98 per cent and 0.35 to 8.73 per cent for NVP, 3TC and d4T,respectively. The contents of NVP, 3TC and d4T in the tablets estimated by HPLC and spectrophotometric methods were similar, and the variation in the amount of these drugs estimated by HPLC and spectrophotometric methods was below 10 per cent. This suggests that the spectrophotometric method is as accurate as the HPLC method for estimation of NVP, 3TC and d4T in tablet/capsule. Hence laboratories that do not have

  9. Built-In Diagnostics (BID) Of Equipment/Systems

    NASA Technical Reports Server (NTRS)

    Granieri, Michael N.; Giordano, John P.; Nolan, Mary E.

    1995-01-01

    Diagnostician(TM)-on-Chip (DOC) technology identifies faults and commands systems reconfiguration. Smart microcontrollers operating in conjunction with other system-control circuits, command self-correcting system/equipment actions in real time. DOC microcontroller generates commands for associated built-in test equipment to stimulate unit of equipment diagnosed, collects and processes response data obtained by built-in test equipment, and performs diagnostic reasoning on response data, using diagnostic knowledge base derived from design data.

  10. 14 CFR 23.1309 - Equipment, systems, and installations.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... environmental conditions, including the indirect effects of lightning strikes. (2) Any equipment and system does... Equipment General § 23.1309 Equipment, systems, and installations. The requirements of this section, except... requirements of part 23, to any equipment or system as installed in the airplane. This section is a regulation...

  11. 14 CFR 23.1309 - Equipment, systems, and installations.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... environmental conditions, including the indirect effects of lightning strikes. (2) Any equipment and system does... Equipment General § 23.1309 Equipment, systems, and installations. The requirements of this section, except... requirements of part 23, to any equipment or system as installed in the airplane. This section is a regulation...

  12. Validation of GC and HPLC systems for residue studies

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Williams, M.

    1995-12-01

    For residue studies, GC and HPLC system performance must be validated prior to and during use. One excellent measure of system performance is the standard curve and associated chromatograms used to construct that curve. The standard curve is a model of system response to an analyte over a specific time period, and is prima facia evidence of system performance beginning at the auto sampler and proceeding through the injector, column, detector, electronics, data-capture device, and printer/plotter. This tool measures the performance of the entire chromatographic system; its power negates most of the benefits associated with costly and time-consuming validation ofmore » individual system components. Other measures of instrument and method validation will be discussed, including quality control charts and experimental designs for method validation.« less

  13. HPLC-Assisted Automated Oligosaccharide Synthesis: Implementation of the Autosampler as a Mode of the Reagent Delivery.

    PubMed

    Pistorio, Salvatore G; Nigudkar, Swati S; Stine, Keith J; Demchenko, Alexei V

    2016-10-07

    The development of a useful methodology for simple, scalable, and transformative automation of oligosaccharide synthesis that easily interfaces with existing methods is reported. The automated synthesis can now be performed using accessible equipment where the reactants and reagents are delivered by the pump or the autosampler and the reactions can be monitored by the UV detector. The HPLC-based platform for automation is easy to setup and adapt to different systems and targets.

  14. 40 CFR 86.1326-90 - Calibration of other equipment.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... equipment requiring calibration is the gas chromatograph and flame ionization detector used in measuring methanol and the high pressure liquid chromatograph (HPLC) and ultraviolet detector for measuring...

  15. 40 CFR 86.1326-90 - Calibration of other equipment.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... equipment requiring calibration is the gas chromatograph and flame ionization detector used in measuring methanol and the high pressure liquid chromatograph (HPLC) and ultraviolet detector for measuring...

  16. 40 CFR 86.1326-90 - Calibration of other equipment.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... equipment requiring calibration is the gas chromatograph and flame ionization detector used in measuring methanol and the high pressure liquid chromatograph (HPLC) and ultraviolet detector for measuring...

  17. 40 CFR 86.1326-90 - Calibration of other equipment.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... equipment requiring calibration is the gas chromatograph and flame ionization detector used in measuring methanol and the high pressure liquid chromatograph (HPLC) and ultraviolet detector for measuring...

  18. 40 CFR 86.126-90 - Calibration of other equipment.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... according to good practice. Specific equipment requiring calibration are the gas chromatograph and flame ionization detector used in measuring methanol and the high pressure liquid chromatograph (HPLC) and...

  19. 40 CFR 86.526-90 - Calibration of other equipment.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... necessary according to good practice. Specific equipment requiring calibration is the gas chromatograph and flame ionization detector used in measuring methanol and the high pressure liquid chromatograph (HPLC...

  20. 40 CFR 86.126-90 - Calibration of other equipment.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... according to good practice. Specific equipment requiring calibration are the gas chromatograph and flame ionization detector used in measuring methanol and the high pressure liquid chromatograph (HPLC) and...

  1. 40 CFR 86.526-90 - Calibration of other equipment.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... necessary according to good practice. Specific equipment requiring calibration is the gas chromatograph and flame ionization detector used in measuring methanol and the high pressure liquid chromatograph (HPLC...

  2. An on-line monitoring system for navigation equipment

    NASA Astrophysics Data System (ADS)

    Wang, Bo; Yang, Ping; Liu, Jing; Yang, Zhengbo; Liang, Fei

    2017-10-01

    Civil air navigation equipment is the most important infrastructure of Civil Aviation, which is closely related to flight safety. In addition to regular flight inspection, navigation equipment's patrol measuring, maintenance measuring, running measuring under special weather conditions are the important means of ensuring aviation flight safety. According to the safety maintenance requirements of Civil Aviation Air Traffic Control navigation equipment, this paper developed one on-line monitoring system with independent intellectual property rights for navigation equipment, the system breakthroughs the key technologies of measuring navigation equipment on-line including Instrument Landing System (ILS) and VHF Omni-directional Range (VOR), which also meets the requirements of navigation equipment ground measurement set by the ICAO DOC 8071, it provides technical means of the ground on-line measurement for navigation equipment, improves the safety of navigation equipment operation, and reduces the impact of measuring navigation equipment on airport operation.

  3. Optical system for UV-laser technological equipment

    NASA Astrophysics Data System (ADS)

    Fedosov, Yuri V.; Romanova, Galina E.; Afanasev, Maxim Ya.

    2017-09-01

    Recently there has been an intensive development of intelligent industrial equipment that is highly automated and can be rapidly adjusted for certain details. This equipment can be robotics systems, automatic wrappers and markers, CNC machines and 3D printers. The work equipment considered is the system for selective curing of photopolymers using a UV-laser and UV-radiation in such equipment that leads to additional technical difficulties. In many cases for transporting the radiation from the laser to the point processed, a multi-mirror system is used: however, such systems are usually difficult to adjust. Additionally, such multi-mirror systems are usually used as a part of the equipment for laser cutting of metals using high-power IR-lasers. For the UV-lasers, using many mirrors leads to crucial radiation losses because of many reflections. Therefore, during the development of the optical system for technological equipment using UV-laser we need to solve two main problems: to transfer the radiation for the working point with minimum losses and to include the system for controlling/handling the radiation spot position. We introduce a system for working with UV-lasers with 450mW of power and a wavelength of 0.45 μm based on a fiber system. In our modelling and design, we achieve spot sizes of about 300 μm, and the designed optical and mechanical systems (prototypes) were manufactured and assembled. In this paper, we present the layout of the technological unit, the results of the theoretical modelling of some parts of the system and some experimental results.

  4. A pretreatment method for HPLC analysis of cypermethrin in microbial degradation systems.

    PubMed

    Liu, Shuliang; Yao, Kai; Jia, Dongying; Zhao, Nan; Lai, Wen; Yuan, Huaiyu

    2012-07-01

    In this paper, a pretreatment method for high-performance liquid chromatography (HPLC) determination of cypermethrin (CY) in microbial degradation systems was systemically studied, primarily to solve the problem of inaccurate determination of CY concentration caused by its uneven distribution in the systems. A suitable pretreatment method was established, including sampling, extraction and dehydration of CY. Partial sampling could be taken for bacterial and yeast systems in which CY was uniformly dispersed by an emulsifying agent, while total sampling was only suitable for mold systems with or without an emulsifying agent. CY could be fully extracted from the samples in which microbial cells were disrupted by ultrasonic treatment with acetonitrile under ultrasonic condition. The extract could be effectively dehydrated and purified by passing it through an anhydrous Na(2)SO(4) column followed by an elution with acetonitrile. The determination of CY in the pretreated sample by HPLC showed a high precision [relative standard deviation (RSD) = 1.14%, n = 5] and a good stability over a period of five days (RSD = 1.57%, n = 5). The recoveries of CY in microbial degradation systems at three different spiked levels ranged from 95.68 to 108.09% (RSD = 0.50-5.87%, n = 5).

  5. [Application of information management system about medical equipment].

    PubMed

    Hang, Jianjin; Zhang, Chaoqun; Wu, Xiang-Yang

    2011-05-01

    Based on the practice of workflow, information management system about medical equipment was developed and its functions such as gathering, browsing, inquiring and counting were introduced. With dynamic and complete case management of medical equipment, the system improved the management of medical equipment.

  6. [Design of medical equipment service management system].

    PubMed

    Jiang, Youhao; PengWen; Jiang, Ningfeng; Ma, Li; Kong, Lingwei; Yin, PeiHao; Sun, Cheng

    2012-09-01

    To develop a maintenance management system for medical equipment based on HIS. The system contains some special functions( including preventive maintenance, automatic job dispatch, performance assessment, etc.) which are very useful for confirming the medical equipment in proper conditions and promoting the working efficiency of the staff. The system provides technical support for the improvement of the maintenance management level. The system, completed the software design using C/S, B/S combination mode. The system realized clients of various sections of zero maintenance, and make the data manipulation, statistical features of equipment management department more convenient. the system connects the subsystems closer and interacts information from time to time, forming a tight network structure. This provides a basis for future hospital-wide information integration.

  7. 33 CFR 105.250 - Security systems and equipment maintenance.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... systems must be regularly tested in accordance with the manufacturers' recommendations; noted deficiencies... 33 Navigation and Navigable Waters 1 2010-07-01 2010-07-01 false Security systems and equipment... systems and equipment maintenance. (a) Security systems and equipment must be in good working order and...

  8. A nitromethane-based HPLC system alternative to acetonitrile for carotenoid analysis of fruit and vegetables.

    PubMed

    Sandmann, Gerhard

    2010-01-01

    Acetonitrile-based HPLC systems are the most commonly used for carotenoid analysis from different plant tissues. Because of the acetonitrile shortage, an HPLC system for the separation of carotenoids on C(18) reversed-phase columns was developed in which an acetonitrile-alcohol-based mobile phase was replaced by nitromethane. This solvent comes closest to acetonitrile with respect to its elutrophic property. Our criterion was to obtain similar separation and retention times for a range of differently structured carotenoids. This was achieved by further increase in the lipophilicity with ethylacetate. For all the carotenoids which we tested, we found co-elution only of β-cryptoxanthin and lycopene. By addition of 1% of water, separation of this pair of carotenoids was also achieved. The final recommended mobile phase consisted of nitromethane : 2-propanol : ethyl acetate : water (79 : 10 : 10 : 1, by volume). On Nucleosil C(18) columns and related ones like Hypersil C(18), we obtained separation of carotenes, hydroxyl, epoxy and keto derivatives, which resembles the excellent separation properties of acetonitrile-based mobile phases on C(18) reversed phase columns. We successfully applied the newly developed HPLC system to the separation of carotenoids from different vegetables and fruit. Copyright © 2010 John Wiley & Sons, Ltd.

  9. 33 CFR 104.260 - Security systems and equipment maintenance.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 33 Navigation and Navigable Waters 1 2010-07-01 2010-07-01 false Security systems and equipment... systems and equipment maintenance. (a) Security systems and equipment must be in good working order and inspected, tested, calibrated and maintained according to the manufacturer's recommendation. (b) The results...

  10. [Application of HIS Hospital Management System in Medical Equipment].

    PubMed

    Li, Yucheng

    2015-07-01

    To analyze the effect of HIS hospital management system in medical equipment. From April 2012 to 2013 in our hospital 5 100 sets of medical equipment as the control group, another 2013 in our hospital from April 2014 may 100 sets of medical equipment as the study group, comparative analysis of two groups of medical equipment scrap rate, usage, maintenance score and the score of benefit etc. Control group and taken to hospital information system, his research group equipment scrap rate, there was a significant difference, the research group of equipment maintenance score and efficiency scores were higher than those of the control group (P < 0.05), the study group of equipment maintenance score and efficiency scores were higher than those of the control group. HIS hospital management system for medical equipment management has positive clinical application value, can effectively improve the use of medical equipment, it is worth to draw and promote.

  11. Development of augmented reality system for servicing electromechanical equipment

    NASA Astrophysics Data System (ADS)

    Zhukovskiy, Y.; Koteleva, N.

    2018-05-01

    Electromechanical equipment is widely used. It is used in industrial enterprises, in the spheres of public services, in everyday life, etc. Maintenance servicing of electromechanical equipment is an important part of its life cycle. High-quality and timely service can extend the life of the electromechanical equipment. The creation of special systems that simplify the process of servicing electromechanical equipment is an urgent task. Such systems can shorten the time for maintenance of electrical equipment, and, therefore, reduce the cost of maintenance in general. This article presents an analysis of information on the operation of service services for maintenance and repair of electromechanical equipment, identifies the list of services, and estimates the time required to perform basic service operations. The structure of the augmented reality system is presented, the ways of interaction of the augmented reality system with the automated control systems working at the enterprise are presented.

  12. [Development of Hospital Equipment Maintenance Information System].

    PubMed

    Zhou, Zhixin

    2015-11-01

    Hospital equipment maintenance information system plays an important role in improving medical treatment quality and efficiency. By requirement analysis of hospital equipment maintenance, the system function diagram is drawed. According to analysis of input and output data, tables and reports in connection with equipment maintenance process, relationships between entity and attribute is found out, and E-R diagram is drawed and relational database table is established. Software development should meet actual process requirement of maintenance and have a friendly user interface and flexible operation. The software can analyze failure cause by statistical analysis.

  13. 14 CFR 29.1309 - Equipment, systems, and installations.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... effects of lightning strikes on the rotorcraft must be considered. (Secs. 313(a), 601, 603, 604, and 605... Equipment, systems, and installations. (a) The equipment, systems, and installations whose functioning is... functions under any foreseeable operating condition. (b) The rotorcraft systems and associated components...

  14. 14 CFR 29.1309 - Equipment, systems, and installations.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... effects of lightning strikes on the rotorcraft must be considered. (Secs. 313(a), 601, 603, 604, and 605... Equipment, systems, and installations. (a) The equipment, systems, and installations whose functioning is... functions under any foreseeable operating condition. (b) The rotorcraft systems and associated components...

  15. 14 CFR 27.1309 - Equipment, systems, and installations.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... showing compliance with paragraph (a), (b), or (c) of this section, the effects of lightning strikes on..., systems, and installations. (a) The equipment, systems, and installations whose functioning is required by... under any foreseeable operating condition. (b) The equipment, systems, and installations of a...

  16. 14 CFR 27.1309 - Equipment, systems, and installations.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... showing compliance with paragraph (a), (b), or (c) of this section, the effects of lightning strikes on..., systems, and installations. (a) The equipment, systems, and installations whose functioning is required by... under any foreseeable operating condition. (b) The equipment, systems, and installations of a...

  17. Automated measurement of carbohydrate-deficient transferrin using the Bio-Rad %CDT by the HPLC test on a Variant HPLC system: evaluation and comparison with other routine procedures.

    PubMed

    Schellenberg, François; Mennetrey, Louise; Girre, Catherine; Nalpas, Bertrand; Pagès, Jean Christophe

    2008-01-01

    In this study, we evaluated the new %CDT by the HPLC method (Bio-Rad, Germany) on a Varianttrade mark HPLC system (Bio-Rad), checked the correlation with well-known methods and calculated the diagnostic value of the test. Intra-run and day-to-day precision values were calculated for samples with extreme serum transferrin concentrations, high trisialotransferrin and interfering conditions (haemolysed, lactescent and icteric samples). The method was compared with two routine procedures, the %CDT TIA (Bio-Rad, Hercules, CA, USA) and the Capillarystrade mark CDT (Sebia, France). A total of 350 clinical sera samples were used for a case-control study. Precision values were better in high CDT and medium CDT pools than in low CDT pools. The serum transferrin concentration had no effect on CDT measurement, except in samples with serum transferrin <1 g/L. Haemolysis was the only interfering situation. The method showed high correlation (r(2) > 0.95) with the two other methods (%CDT TIA and CZE %CDT). The global predictive value of the test was >0.90 at 1.9% cut-off. These results demonstrate that the %CDT by the HPLC test is suitable for CDT routine measurement; the results from the high-throughput Varianttrade mark system are well correlated with other methods and are of high diagnostic value.

  18. 14 CFR 25.1309 - Equipment, systems, and installations.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... TRANSPORTATION AIRCRAFT AIRWORTHINESS STANDARDS: TRANSPORT CATEGORY AIRPLANES Equipment General § 25.1309... electrical system and equipment design and installation, critical environmental conditions must be considered. For electrical generation, distribution, and utilization equipment required by or used in complying...

  19. 75 FR 47036 - Small Business Size Standards: Waiver of the Nonmanufacturer Rule

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-08-04

    ... Spectrometry (CS-MS) Systems, High Performance Liquid Chromatography (HPLC) Systems, Gas Chromatography Mass...-portable CS-MS, HPLC, GC-MS, and, ICP-MS Laboratory Equipment under PSC 6640 (Laboratory Equipment and...-portable equipment, SBA is limiting the waiver to not-portable CS-MS Systems, HPLC Systems, GC-MS Systems...

  20. High-speed railway signal trackside equipment patrol inspection system

    NASA Astrophysics Data System (ADS)

    Wu, Nan

    2018-03-01

    High-speed railway signal trackside equipment patrol inspection system comprehensively applies TDI (time delay integration), high-speed and highly responsive CMOS architecture, low illumination photosensitive technique, image data compression technique, machine vision technique and so on, installed on high-speed railway inspection train, and achieves the collection, management and analysis of the images of signal trackside equipment appearance while the train is running. The system will automatically filter out the signal trackside equipment images from a large number of the background image, and identify of the equipment changes by comparing the original image data. Combining with ledger data and train location information, the system accurately locate the trackside equipment, conscientiously guiding maintenance.

  1. 49 CFR 179.20 - Service equipment; protection systems.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 49 Transportation 3 2011-10-01 2011-10-01 false Service equipment; protection systems. 179.20 Section 179.20 Transportation Other Regulations Relating to Transportation (Continued) PIPELINE AND... CARS General Design Requirements § 179.20 Service equipment; protection systems. If an applicable tank...

  2. NASA ground terminal communication equipment automated fault isolation expert systems

    NASA Technical Reports Server (NTRS)

    Tang, Y. K.; Wetzel, C. R.

    1990-01-01

    The prototype expert systems are described that diagnose the Distribution and Switching System I and II (DSS1 and DSS2), Statistical Multiplexers (SM), and Multiplexer and Demultiplexer systems (MDM) at the NASA Ground Terminal (NGT). A system level fault isolation expert system monitors the activities of a selected data stream, verifies that the fault exists in the NGT and identifies the faulty equipment. Equipment level fault isolation expert systems are invoked to isolate the fault to a Line Replaceable Unit (LRU) level. Input and sometimes output data stream activities for the equipment are available. The system level fault isolation expert system compares the equipment input and output status for a data stream and performs loopback tests (if necessary) to isolate the faulty equipment. The equipment level fault isolation system utilizes the process of elimination and/or the maintenance personnel's fault isolation experience stored in its knowledge base. The DSS1, DSS2 and SM fault isolation systems, using the knowledge of the current equipment configuration and the equipment circuitry issues a set of test connections according to the predefined rules. The faulty component or board can be identified by the expert system by analyzing the test results. The MDM fault isolation system correlates the failure symptoms with the faulty component based on maintenance personnel experience. The faulty component can be determined by knowing the failure symptoms. The DSS1, DSS2, SM, and MDM equipment simulators are implemented in PASCAL. The DSS1 fault isolation expert system was converted to C language from VP-Expert and integrated into the NGT automation software for offline switch diagnoses. Potentially, the NGT fault isolation algorithms can be used for the DSS1, SM, amd MDM located at Goddard Space Flight Center (GSFC).

  3. 46 CFR 169.253 - Miscellaneous systems and equipment.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 46 Shipping 7 2011-10-01 2011-10-01 false Miscellaneous systems and equipment. 169.253 Section 169.253 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) NAUTICAL SCHOOLS SAILING SCHOOL VESSELS Inspection and Certification Inspections § 169.253 Miscellaneous systems and equipment. (a) At...

  4. 46 CFR 169.253 - Miscellaneous systems and equipment.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 46 Shipping 7 2010-10-01 2010-10-01 false Miscellaneous systems and equipment. 169.253 Section 169.253 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) NAUTICAL SCHOOLS SAILING SCHOOL VESSELS Inspection and Certification Inspections § 169.253 Miscellaneous systems and equipment. (a) At...

  5. Emergency sacrificial sealing method in filters, equipment, or systems

    DOEpatents

    Brown, Erik P

    2014-09-30

    A system seals a filter or equipment component to a base and will continue to seal the filter or equipment component to the base in the event of hot air or fire. The system includes a first sealing material between the filter or equipment component and the base; and a second sealing material between the filter or equipment component and the base and proximate the first sealing material. The first sealing material and the second seal material are positioned relative to each other and relative to the filter or equipment component and the base to seal the filter or equipment component to the base and upon the event of fire the second sealing material will be activated and expand to continue to seal the filter or equipment component to the base in the event of hot air or fire.

  6. Emergency sacrificial sealing method in filters, equipment, or systems

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Brown, Erik P.

    A system seals a filter or equipment component to abase and will continue to seal the filter or equipment component to the base in the event of hot air or fire. The system includes a first sealing material between the filter or equipment component and the base; and a second sealing material between the filter or equipment component and the base and proximate the first sealing material. The first sealing material and the second seal material are positioned relative to each other and relative to the filter or equipment component and the base to seal the filter or equipment componentmore » to the base and upon the event of fire the second sealing material will be activated and expand to continue to seal the filter or equipment component to the base in the event of hot air or fire.« less

  7. Tethered Satellite System (TSS) core equipment

    NASA Technical Reports Server (NTRS)

    Bonifazi, C.

    1986-01-01

    To date, three Tethered Satellite System (TSS) missions of the Italian provided scientific satellite orbiting in the ionosphere connected to U.S. Space Shuttle is foreseen. The first mission will use an electrically conductive tether of 20 km deployed upward from the orbiter flying at 300 km altitude. This mission will allow investigation of the TSS electrodynamic interaction with the ionosphere due to the high voltage induced across the two terminators of the system during its motion throughout the geomagnetic field. The second mission will use a dielectric tether of 100 km deployed downward from the Orbiter flying at 230 km altitude. Tethered-vehicle access to altitude as low as 120 to 150 km from the Orbiter would permit direct long term observation of phenomena in the lower thermosphere and determination of other dynamical physical processes. The third mission would use the same configuration of the first electrodynamic mission with the complete Core Equipment. Study of power generation by tethered systems would be possible by operating the Core Equipment in the inverted current mode. This mode of operation would allow ion current collection upon the TSS satellite by controlling its potential with respect to the ambient ionospheric plasma. The main requirements of the Core Equipment configuration to date foreseen for the first TSS electrodynamic mission is described. Besides the Core Equipment purposes, its hardware and operational sub-modes of operation are described.

  8. Immunoassay screening of lysergic acid diethylamide (LSD) and its confirmation by HPLC and fluorescence detection following LSD ImmunElute extraction.

    PubMed

    Grobosch, T; Lemm-Ahlers, U

    2002-04-01

    In all, 3872 urine specimens were screened for lysergic acid diethylamide (LSD) using the CEDIA DAU LSD assay. Forty-eight samples, mainly from psychiatric patients or drug abusers, were found to be LSD positive, but only 13 (27%) of these could be confirmed by high-performance liquid chromatography with fluorescence detection (HPLC-FLD) following immunoaffinity extraction (IAE). Additional analysis for LSD using the DPC Coat-a-Count RIA was performed to compare the two immunoassay screening methods. Complete agreement between the DPC RIA assay and HPLC-FLD results was observed at concentrations below a cutoff concentration of 500 pg/mL. Samples that were LSD positive in the CEDIA DAU assay but not confirmed by HPLC-FLD were also investigated for interfering compounds using REMEDI HS drug-profiling system. REMEDI HS analysis identified 15 compounds (parent drugs and metabolites) that are believed to cross-react in the CEDIA DAU LSD assay: ambroxol, prilocaine, pipamperone, diphenhydramine, metoclopramide, amitriptyline, doxepine, atracurium, bupivacaine, doxylamine, lidocaine, mepivacaine, promethazine, ranitidine, and tramadole. The IAE/HPLC-FLD combination is rapid, easy to perform and reliable. It can reduce costs when standard, rather than more advanced, HPLC equipment is used, especially for labs that perform analyses for LSD infrequently. The chromatographic analysis of LSD, nor-LSD, and iso-LSD is not influenced by any of the tested cross-reacting compounds even at a concentration of 100 ng/mL.

  9. 33 CFR 106.255 - Security systems and equipment maintenance.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... maintained according to manufacturers' recommendations. (b) Security systems must be regularly tested in... 33 Navigation and Navigable Waters 1 2010-07-01 2010-07-01 false Security systems and equipment... Shelf (OCS) Facility Security Requirements § 106.255 Security systems and equipment maintenance. (a...

  10. 14 CFR 121.358 - Low-altitude windshear system equipment requirements.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 14 Aeronautics and Space 3 2014-01-01 2014-01-01 false Low-altitude windshear system equipment... OPERATING REQUIREMENTS: DOMESTIC, FLAG, AND SUPPLEMENTAL OPERATIONS Instrument and Equipment Requirements § 121.358 Low-altitude windshear system equipment requirements. (a) Airplanes manufactured after January...

  11. 14 CFR 121.358 - Low-altitude windshear system equipment requirements.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 14 Aeronautics and Space 3 2011-01-01 2011-01-01 false Low-altitude windshear system equipment... OPERATING REQUIREMENTS: DOMESTIC, FLAG, AND SUPPLEMENTAL OPERATIONS Instrument and Equipment Requirements § 121.358 Low-altitude windshear system equipment requirements. (a) Airplanes manufactured after January...

  12. 14 CFR 121.358 - Low-altitude windshear system equipment requirements.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 14 Aeronautics and Space 3 2010-01-01 2010-01-01 false Low-altitude windshear system equipment... OPERATING REQUIREMENTS: DOMESTIC, FLAG, AND SUPPLEMENTAL OPERATIONS Instrument and Equipment Requirements § 121.358 Low-altitude windshear system equipment requirements. (a) Airplanes manufactured after January...

  13. 14 CFR 121.358 - Low-altitude windshear system equipment requirements.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 14 Aeronautics and Space 3 2012-01-01 2012-01-01 false Low-altitude windshear system equipment... OPERATING REQUIREMENTS: DOMESTIC, FLAG, AND SUPPLEMENTAL OPERATIONS Instrument and Equipment Requirements § 121.358 Low-altitude windshear system equipment requirements. (a) Airplanes manufactured after January...

  14. 14 CFR 121.358 - Low-altitude windshear system equipment requirements.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 14 Aeronautics and Space 3 2013-01-01 2013-01-01 false Low-altitude windshear system equipment... OPERATING REQUIREMENTS: DOMESTIC, FLAG, AND SUPPLEMENTAL OPERATIONS Instrument and Equipment Requirements § 121.358 Low-altitude windshear system equipment requirements. (a) Airplanes manufactured after January...

  15. Non-invasive system for monitoring of the manufacturing equipment

    NASA Astrophysics Data System (ADS)

    Mazăre, A. G.; Belu, N.; Ionescu, L. M.; Rachieru, N.; Misztal, A.

    2017-08-01

    The automotive industry is one of the most important industries in the world that concerns the economy and the world culture. High demand has resulted in increasing of the pressure on the production lines. In conclusion, it is required more careful in monitoring of the production equipment not only for maintenance but also for staff safety and to increase the quality of production. In this paper, we propose a solution for non-invasive monitoring of the industrial equipment operation by measuring the current consumption on energy supply lines. Thus, it is determined the utilization schedule of the equipment and operation mode. Based on these measurements, it’s built an activity report for that equipment, available to the quality management and maintenance team. The solution consists of the current measuring equipment, with self-harvesting capabilities and radio transceiver, and an embedded system which run a server. The current measuring equipment will transmit data about consumption of each energy supply network line where is placed the industrial equipment. So, we have an internal measuring radio network. The embedded system will collect data for the equipment and put in a local data base and it will provide via an intranet application. The entire system not requires any supplementary energy supply and interventions in the factory infrastructure. It is experimented in a company from the automotive industries.

  16. Research on large equipment maintenance system in life cycle

    NASA Astrophysics Data System (ADS)

    Xu, Xiaowei; Wang, Hongxia; Liu, Zhenxing; Zhang, Nan

    2017-06-01

    In order to change the current disadvantages of traditional large equipment maintenance concept, this article plans to apply the technical method of prognostics and health management to optimize equipment maintenance strategy and develop large equipment maintenance system. Combined with the maintenance procedures of various phases in life cycle, it concluded the formulation methods of maintenance program and implement plans of maintenance work. In the meantime, it takes account into the example of the dredger power system of the Waterway Bureau to establish the auxiliary platform of ship maintenance system in life cycle.

  17. Specialized computer system to diagnose critical lined equipment

    NASA Astrophysics Data System (ADS)

    Yemelyanov, V. A.; Yemelyanova, N. Y.; Morozova, O. A.; Nedelkin, A. A.

    2018-05-01

    The paper presents data on the problem of diagnosing the lining condition at the iron and steel works. The authors propose and describe the structure of the specialized computer system to diagnose critical lined equipment. The relative results of diagnosing lining condition by the basic system and the proposed specialized computer system are presented. To automate evaluation of lining condition and support in making decisions regarding the operation mode of the lined equipment, the specialized software has been developed.

  18. Identification and determination of flavonoids, carotenoids and chlorophyll concentration in Cynodon dactylon (L.) by HPLC analysis.

    PubMed

    Muthukrishnan, Saradha Devi; Kaliyaperumal, Ashokkumar; Subramaniyan, Annapoorani

    2015-01-01

    Cynodon dactylon (L.) is a potent medicinal plant in the traditional and current Indian medicinal systems. The objective of this research was to find out the levels of flavonoids, carotenoids and chlorophyll b in C. dactylon leaves by high-performance liquid chromatography (HPLC) equipped with a diode array detector. HPLC analysis revealed that total carotenoid and total flavonoid concentration were 62 mg/100 g and 249.1 μg/g, respectively. The mean chlorophyll b was 85.1 mg/100 g in C. dactylon. Among the flavonoids, quercetin (164.7 μg/g) was the major flavonoid followed by kaempferol (48.2 μg/g), rutin (18.4 μg/g), catechin (12.1 μg/g) and myricetin (5.7 μg/g). Of the carotenoids, β-carotene (35.2 mg/100 g) was predominant followed by lutein (17.0 mg/100 g), violaxanthin (5.8 mg/100 g) and zeaxanthin (4.2 mg/100 g). Chlorophyll b concentration was 85.1 mg/100 g in C. dactylon. The results of this investigation should be useful information for further pharmacological studies.

  19. Photographic Equipment Test System (PETS)

    NASA Technical Reports Server (NTRS)

    1975-01-01

    The Photographic Equipment Test System is presented. The device is a mobile optical system designed for evaluating performance of various sensors in a laboratory, in a vacuum chamber or on a flight line. The carriage is designed to allow elevation as well as azimuth control of the direction of the light from the collimator. The pneumatic tires provide an effective vibration isolation system. A target/illumination system is mounted on a motor driven linear slide, and focusing and exposure control can be operated remotely from the small electronics control console.

  20. Satellite services system analysis study. Volume 4: Service equipment concepts

    NASA Technical Reports Server (NTRS)

    1981-01-01

    Payload deployment equipment is discussed, including payload separation, retention structures, the remote manipulator system, tilt tables, the payload installation and deployment aid, the handling and positioning aid, and spin tables. Close proximity retrieval, and on-orbit servicing equipment is discussed. Backup and contingency equipment is also discussed. Delivery and retrieval of high-energy payloads are considered. Earth return equipment, the aft flight deck, optional, and advanced equipment are also discussed.

  1. Management information system of medical equipment using mobile devices

    NASA Astrophysics Data System (ADS)

    Núñez, C.; Castro, D.

    2011-09-01

    The large numbers of technologies currently incorporated into mobile devices transform them into excellent tools for capture and to manage the information, because of the increasing computing power and storage that allow to add many miscellaneous applications. In order to obtain benefits of these technologies, in the biomedical engineering field, it was developed a mobile information system for medical equipment management. The central platform for the system it's a mobile phone, which by a connection with a web server, it's capable to send and receive information relative to any medical equipment. Decoding a type of barcodes, known as QR-Codes, the management process is simplified and improved. These barcodes identified the medical equipments in a database, when these codes are photographed and decoded with the mobile device, you can access to relevant information about the medical equipment in question. This Project in it's actual state is a basic support tool for the maintenance of medical equipment. It is also a modern alternative, competitive and economic in the actual market.

  2. Automatic Radiated Susceptibility Test System for Payload Equipment

    NASA Technical Reports Server (NTRS)

    Ngo, Hoai T.; Sturman, John C.; Sargent, Noel B.

    1995-01-01

    An automatic radiated susceptibility test system (ARSTS) was developed for NASA Lewis Research Center's Electro-magnetic Interference laboratory. According to MSFC-SPEC 521B, any electrical or electronic equipment that will be transported by the spacelab and space shuttle must be tested for susceptibility to electromagnetic interference. This state-of-the-art automatic test system performs necessary calculations; analyzes, processes, and records a great quantity of measured data; and monitors the equipment being tested in real-time and with minimal user intervention. ARSTS reduces costly test time, increases test accuracy, and provides reliable test results.

  3. GEECS (Generalized Equipment and Experiment Control System)

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    GONSALVES, ANTHONY; DESHMUKH, AALHAD

    2017-01-12

    GEECS (Generalized Equipment and Experiment Control System) monitors and controls equipment distributed across a network, performs experiments by scanning input variables, and collects and stores various types of data synchronously from devices. Examples of devices include cameras, motors and pressure gauges. GEEKS is based upon LabView graphical object oriented programming (GOOP), allowing for a modular and scalable framework. Data is published for subscription of an arbitrary number of variables over TCP. A secondary framework allows easy development of graphical user interfaces for a combined control of any available devices on the control system without the need of programming knowledge. Thismore » allows for rapid integration of GEECS into a wide variety of systems. A database interface provides for devise and process configuration while allowing the user to save large quantities of data to local or network drives.« less

  4. Automated system for definition of life-cycle resources of electromechanical equipment

    NASA Astrophysics Data System (ADS)

    Zhukovskiy, Y.; Koteleva, N.

    2017-02-01

    The frequency of maintenance of electromechanical equipment depends on the plant, which uses and runs this equipment. Very often the maintenance frequency is poorly correlated with the actual state of the electromechanical equipment. Furthermore, traditional methods of diagnosis sometimes cannot work without stopping the process (for example, for equipment located in hard to reach places) and so the maintenance costs are increased. This problem can be solved using the indirect methods of diagnosing of the electromechanical equipment. The indirect methods often use the parameters in the real time and seldom use the parameters of traditional diagnostic methods for determination of the resource of electromechanical equipment. This article is dedicated to developing the structure of a special automated control system. This system must use the big flow of the information about the direct and indirect parameters of the equipment state from plants from different areas of industry and factories which produce the electromechanical equipment.

  5. 42 CFR 493.1252 - Standard: Test systems, equipment, instruments, reagents, materials, and supplies.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 42 Public Health 5 2010-10-01 2010-10-01 false Standard: Test systems, equipment, instruments... REQUIREMENTS Quality System for Nonwaived Testing Analytic Systems § 493.1252 Standard: Test systems, equipment...) Temperature. (3) Humidity. (4) Protection of equipment and instruments from fluctuations and interruptions in...

  6. The Logistics Equipment Carbon Emission Monitoring System for a Green Logistics

    NASA Astrophysics Data System (ADS)

    Choi, Hyungrim; Park, Byoungkwon; Lee, Byungha; Park, Yongsung; Lee, Changsup; Ha, Jeongsoo

    Recently, due to the global enforcement of obligations to reduce green house gases and various environmental regulations, low carbon green growth strategies are required. Currently, in our country, environment friendly logistics activities are staying in the early stage compared to advanced countries because of our country's large energy consumption type industrial structures. As a measure to respond to the trend of the reinforcement of international environmental regulations in the sector of logistics, active green logistics systems should be established and to solve this problem, this study is intended to develop a monitoring system that can manage the carbon emission of logistics equipment(container truck, discharging equipment etc) in real time using a new technology named IP-RFID. The monitoring system developed in this study can actively manage the carbon emission of individual logistics equipment by attaching IP-Tags that can measure the carbon emission of individual logistics equipment in real time and transmit the information obtained from the measurement directly to users through IP communication. Since carbon emission can be managed by logistics equipment and drivers can check the carbon emission of equipment through this system, the carbon emission generated in the logistics sector may be reduced by using this system.

  7. 78 FR 38093 - Seventh Meeting: RTCA Special Committee 226, Audio Systems and Equipment

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-06-25

    ... Committee 226, Audio Systems and Equipment AGENCY: Federal Aviation Administration (FAA), U.S. Department of Transportation (DOT). ACTION: Meeting Notice of RTCA Special Committee 226, Audio Systems and Equipment. SUMMARY... 226, Audio Systems and Equipment [[Page 38094

  8. 14 CFR 23.1309 - Equipment, systems, and installations.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... compliance with this section with regard to the electrical power system and to equipment design and... the system must be able to supply the following power loads in probable operating combinations and for probable durations: (1) Loads connected to the power distribution system with the system functioning...

  9. HPLC studio: a novel software utility to perform HPLC chromatogram comparison for screening purposes.

    PubMed

    García, J B; Tormo, José R

    2003-06-01

    A new tool, HPLC Studio, was developed for the comparison of high-performance liquid chromatography (HPLC) chromatograms from microbial extracts. The new utility makes it possible to create a virtual chromatogram by mixing up to 20 individual chromatograms. The virtual chromatogram is the first step in establishing a ranking of the microbial fermentation conditions based on either the area or diversity of HPLC peaks. The utility was used to maximize the diversity of secondary metabolites tested from a microorganism and therefore increase the chances of finding new lead compounds in a drug discovery program.

  10. Simultaneous determination of five coumarins in Angelicae dahuricae Radix by HPLC/UV and LC-ESI-MS/MS.

    PubMed

    Park, Ah Yeon; Park, So-Young; Lee, Jaehyun; Jung, Mihye; Kim, Jinwoong; Kang, Sam Sik; Youm, Jeong-Rok; Han, Sang Beom

    2009-10-01

    Rapid, simple and reliable HPLC/UV and LC-ESI-MS/MS methods for the simultaneous determination of five active coumarins of Angelicae dahuricae Radix, byakangelicol (1), oxypeucedanin (2), imperatorin (3), phellopterin (4) and isoimperatorin (5) were developed and validated. The separation condition for HPLC/UV was optimized using a Develosil RPAQUEOUS C(30) column using 70% acetonitrile in water as the mobile phase. This HPLC/UV method was successful for providing the baseline separation of the five coumarins with no interfering peaks detected in the 70% ethanol extract of Angelicae dahuricae Radix. The specific determination of the five coumarins was also accomplished by a triple quadrupole tandem mass spectrometer equipped with an electrospray ionization source (LC-ESI-MS/MS). Multiple reaction monitoring (MRM) in the positive mode was used to enhance the selectivity of detection. The LC-ESI-MS/MS methods were successfully applied for the determination of the five major coumarins in Angelicae dahuricae Radix. These HPLC/UV and LC-ESI-MS/MS methods were validated in terms of recovery, linearity, accuracy and precision (intra- and inter-day validation). Taken together, the shorter analysis time involved makes these HPLC/UV and LC-ESI-MS/MS methods valuable for the commercial quality control of Angelicae dahuricae Radix extracts and its pharmaceutical preparations. Copyright (c) 2009 John Wiley & Sons, Ltd.

  11. HPLC-ESI-QTOF-MS as a powerful analytical tool for characterising phenolic compounds in olive-leaf extracts.

    PubMed

    Quirantes-Piné, Rosa; Lozano-Sánchez, Jesús; Herrero, Miguel; Ibáñez, Elena; Segura-Carretero, Antonio; Fernández-Gutiérrez, Alberto

    2013-01-01

    Olea europaea L. leaves may be considered a cheap, easily available natural source of phenolic compounds. In a previous study we evaluated the possibility of obtaining bioactive phenolic compounds from olive leaves by pressurised liquid extraction (PLE) for their use as natural anti-oxidants. The alimentary use of these kinds of extract makes comprehensive knowledge of their composition essential. To undertake a comprehensive characterisation of two olive-leaf extracts obtained by PLE using high-performance liquid chromatography coupled to electrospray ionisation and quadrupole time-of-flight mass spectrometry (HPLC-ESI-QTOF-MS). Olive leaves were extracted by PLE using ethanol and water as extraction solvents at 150°C and 200°C respectively. Separation was carried out in a HPLC system equipped with a C₁₈-column working in a gradient elution programme coupled to ESI-QTOF-MS operating in negative ion mode. This analytical platform was able to detect 48 compounds and tentatively identify 31 different phenolic compounds in these extracts, including secoiridoids, simple phenols, flavonoids, cinnamic-acid derivatives and benzoic acids. Lucidumoside C was also identified for the first time in olive leaves. The coupling of HPLC-ESI-QTOF-MS led to the in-depth characterisation of the olive-leaf extracts on the basis of mass accuracy, true isotopic pattern and tandem mass spectrometry (MS/MS) spectra. We may conclude therefore that this analytical tool is very valuable in the study of phenolic compounds in plant matrices. Copyright © 2012 John Wiley & Sons, Ltd.

  12. Army Logistics Systems: Opportunities to Improve the Accuracy of the Army’s Major Equipment Item System

    DTIC Science & Technology

    1998-01-01

    consolidated financial statements of the federal government, we are reviewing the accuracy of data in the military services’ financial/logistical systems that are used to provide property, plant, and equipment information to the financial statements. The Army uses the Continuing Balance System-Expanded (CBSX) as its central logistics system for reporting the types, quantities, and location of equipment; monitoring the equipment readiness of its warfighting units; and filling equipment shortages in those units scheduled for mobilization. In addition,

  13. Application of a small-scale equipment system for biomass harvesting

    Treesearch

    Aihua Yu; Tom Gallagher; Dana Mitchell; Brandon O' Neal

    2016-01-01

    The small-scale harvesting equipment system has been and continues to grow in use in forestry operations in some regions in the world. This harvest system can include a range of equipment types, such as feller-bunchers or chainsaws, skidders or farm tractors, and chippers. These machines are generally smaller, lower cost and less productive than larger, more advanced...

  14. Machine protection system for rotating equipment and method

    DOEpatents

    Lakshminarasimha, Arkalgud N.; Rucigay, Richard J.; Ozgur, Dincer

    2003-01-01

    A machine protection system and method for rotating equipment introduces new alarming features and makes use of full proximity probe sensor information, including amplitude and phase. Baseline vibration amplitude and phase data is estimated and tracked according to operating modes of the rotating equipment. Baseline vibration and phase data can be determined using a rolling average and variance and stored in a unit circle or tracked using short term average and long term average baselines. The sensed vibration amplitude and phase is compared with the baseline vibration amplitude and phase data. Operation of the rotating equipment can be controlled based on the vibration amplitude and phase.

  15. [HPLC fingerprint of Calendula officinalis flower].

    PubMed

    Xing, Zhan-Fen; Cheng, Hong-Da; Zhang, Ping-Ping; Gong, Lei; Ma, Li-Ya

    2014-07-01

    To establish an HPLC fingerprint of Calendula officinalis flower for its quality control. Hypersil ODS C18 column (250 mm x 4.6 mm, 5 μm) was used with acetonitrile and water as mobile phase in a gradient mode at the flow rate of 1.0 mL/min. The detection wavelength was 220 nm and the temperature of column was set at 35 degrees C. The similarity was analyzed with the Estimating System of Similarity on the Chinese Medicine Fingerprint Chromatogram. The HPLC fingerprint of Calendula officinalis flower containing eleven peaks was set up. The similarity of Calendula officinalis flower from different habitats was greater than 0.90. This method is easy and reliable, which can be used to judge the habitat and control the quality of Calendula officinalis flower.

  16. 78 FR 18416 - Sixth Meeting: RTCA Special Committee 226, Audio Systems and Equipment

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-03-26

    ... 226, Audio Systems and Equipment AGENCY: Federal Aviation Administration (FAA), U.S. Department of Transportation (DOT). ACTION: Meeting Notice of RTCA Special Committee 226, Audio Systems and Equipment. SUMMARY... 226, Audio Systems and Equipment. DATES: The meeting will be held April 15-17, 2013 from 9:00 a.m.-5...

  17. 78 FR 57673 - Eighth Meeting: RTCA Special Committee 226, Audio Systems and Equipment

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-09-19

    ... Committee 226, Audio Systems and Equipment AGENCY: Federal Aviation Administration (FAA), U.S. Department of Transportation (DOT). ACTION: Meeting Notice of RTCA Special Committee 226, Audio Systems and Equipment. SUMMARY... Committee 226, Audio Systems and Equipment. DATES: The meeting will be held October 8-10, 2012 from 9:00 a.m...

  18. 77 FR 37732 - Fourteenth Meeting: RTCA Special Committee 224, Audio Systems and Equipment

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-06-22

    ... Committee 224, Audio Systems and Equipment AGENCY: Federal Aviation Administration (FAA), U.S. Department of Transportation (DOT). ACTION: Meeting Notice of RTCA Special Committee 224, Audio Systems and Equipment. SUMMARY... Committee 224, Audio Systems and Equipment. DATES: The meeting will be held July 11, 2012, from 10 a.m.-4 p...

  19. Crew equipment applications - Firefighter's Breathing System.

    NASA Technical Reports Server (NTRS)

    Smith, W. L.

    1973-01-01

    The Firefighter's Breathing System (FBS) represents a significant step in applying NASA's crew equipment technologists and technologies to civilian sector problems. This paper describes the problem, the utilization of user-design committees as a forum for development of design goals, the design of the FBS, and the field test program to be conducted.

  20. Rail Transit System Maintenance Practices for Automatic Fare Collection Equipment

    DOT National Transportation Integrated Search

    1984-05-01

    A review of rail transit system maintenance practices for automatic fare collection (AFC) equipment was performed. This study supports an UMTA sponsored program to improve the reliability of AFC equipment. The maintenance practices of the transit sys...

  1. Methods and applications of HPLC-AMS (WBio 5)

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Bucholz, B A; Clifford, A J; Duecker, S R

    Pharmacokinetics of physiologic doses of nutrients, pesticides, and herbicides can easily be traced in humans using a {sup 14}C-labelled compound. Basic kinetics can be monitored in blood or urine by measuring the elevation in the {sup 14}C content above the control predose tissue and converting to equivalents of the parent compound. High Performance Liquid Chromatography (HPLC) is an excellent method for the chemical separation of complex mixtures whose profiles afford estimation of biochemical pathways of metabolism. Compounds elute from the HPLC systems with characteristic retention times and can be collected in fractions that can then be graphitized for AMS measurement.more » Unknowns are identified by coelution with known standards and chemical tests that reveal functional groupings. Metabolites are quantified with the {sup 14}C signal. Thoroughly accounting for the carbon inventory in the LC solvents, ion-pairing agents, samples, and carriers adds some complexity to the analysis. In most cases the total carbon inventory is dominated by carrier. Baseline background and stability need to be carefully monitored. Limits of quantitation near 10 amol of {sup 14}C per HPLC fraction are typically achieved. Baselines are maintained by limiting injected {sup 14}C activity <0.17 Bq (4.5 pCi) on the HPLC column.« less

  2. [A new HPLC procedure for cyclamate in food with pre-chromatographic derivatization].

    PubMed

    Schwedt, G; Hauck, M

    1988-08-01

    A high-pressure liquid chromatography (HPLC) procedure for the detection of cyclamate in liquid and solid samples is presented, which depends on oxidation and the reaction of cyclohexylamine with o-phthaldialdehyde to form a condensation product. The results of the HPLC analysis, using an RP-C 18 separation system with UV detection at 242 nm are reported. Contents, from 2 to 400 mg/l, can be detected in less than 2 h (HPLC analysis within 20 min) with relative standard deviations of 4%. Only for cucumber infusions were incomplete recoveries of 68% obtained.

  3. Deep Space Network equipment performance, reliability, and operations management information system

    NASA Technical Reports Server (NTRS)

    Cooper, T.; Lin, J.; Chatillon, M.

    2002-01-01

    The Deep Space Mission System (DSMS) Operations Program Office and the DeepSpace Network (DSN) facilities utilize the Discrepancy Reporting Management System (DRMS) to collect, process, communicate and manage data discrepancies, equipment resets, physical equipment status, and to maintain an internal Station Log. A collaborative effort development between JPL and the Canberra Deep Space Communication Complex delivered a system to support DSN Operations.

  4. 14 CFR 25.1445 - Equipment standards for the oxygen distributing system.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 14 Aeronautics and Space 1 2014-01-01 2014-01-01 false Equipment standards for the oxygen... Miscellaneous Equipment § 25.1445 Equipment standards for the oxygen distributing system. (a) When oxygen is... crew on duty. (b) Portable walk-around oxygen units of the continuous flow, diluter-demand, and...

  5. 14 CFR 25.1445 - Equipment standards for the oxygen distributing system.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 14 Aeronautics and Space 1 2011-01-01 2011-01-01 false Equipment standards for the oxygen... Miscellaneous Equipment § 25.1445 Equipment standards for the oxygen distributing system. (a) When oxygen is... crew on duty. (b) Portable walk-around oxygen units of the continuous flow, diluter-demand, and...

  6. 14 CFR 25.1445 - Equipment standards for the oxygen distributing system.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 14 Aeronautics and Space 1 2012-01-01 2012-01-01 false Equipment standards for the oxygen... Miscellaneous Equipment § 25.1445 Equipment standards for the oxygen distributing system. (a) When oxygen is... crew on duty. (b) Portable walk-around oxygen units of the continuous flow, diluter-demand, and...

  7. 14 CFR 25.1445 - Equipment standards for the oxygen distributing system.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 14 Aeronautics and Space 1 2010-01-01 2010-01-01 false Equipment standards for the oxygen... Miscellaneous Equipment § 25.1445 Equipment standards for the oxygen distributing system. (a) When oxygen is... crew on duty. (b) Portable walk-around oxygen units of the continuous flow, diluter-demand, and...

  8. 14 CFR 25.1445 - Equipment standards for the oxygen distributing system.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 14 Aeronautics and Space 1 2013-01-01 2013-01-01 false Equipment standards for the oxygen... Miscellaneous Equipment § 25.1445 Equipment standards for the oxygen distributing system. (a) When oxygen is... crew on duty. (b) Portable walk-around oxygen units of the continuous flow, diluter-demand, and...

  9. Hybrid Damping System for an Electronic Equipment Mounting Shelf

    NASA Technical Reports Server (NTRS)

    Voracek, David; Kolkailah, Faysal A.; Cavalli, J. R.; Elghandour, Eltahry

    1997-01-01

    The objective of this study was to design and construct a vibration control system for an electronic equipment shelf to be evaluated in the NASA Dryden FTF-II. The vibration control system was a hybrid system which included passive and active damping techniques. Passive damping was fabricated into the equipment shelf using ScothDamp(trademark) damping film and aluminum constraining layers. Active damping was achieved using a two channel active control circuit employing QuickPack(trademark) sensors and actuators. Preliminary Chirp test results indicated passive damping smoothed the frequency response while active damping reduced amplitudes of the frequency response for most frequencies below 500Hz.

  10. Hybrid Damping System for an Electronic Equipment Mounting Shelf

    NASA Technical Reports Server (NTRS)

    Voracek, David; Kolkailah, Faysal A.; Cavalli, J. R.; Elghandour, Eltahry

    1997-01-01

    The objective of this study was to design and construct a vibration control system for an electronic equipment shelf to be evaluated in the NASA Dryden FTF-11. The vibration control system was a hybrid system which included passive and active damping techniques. Passive damping was fabricated into the equipment shelf using ScothDamp(trademark) damping film and aluminum constraining layers. Active damping was achieved using a two channel active control circuit employing QuickPack(trademark) sensors and actuators. Preliminary Chirp test results indicated passive damping smoothed the frequency response while active damping reduced amplitudes of the frequency response for most frequencies below 500Hz.

  11. Design and Prototype of an Automated Column-Switching HPLC System for Radiometabolite Analysis.

    PubMed

    Vasdev, Neil; Collier, Thomas Lee

    2016-08-17

    Column-switching high performance liquid chromatography (HPLC) is extensively used for the critical analysis of radiolabeled ligands and their metabolites in plasma. However, the lack of streamlined apparatus and consequently varying protocols remain as a challenge among positron emission tomography laboratories. We report here the prototype apparatus and implementation of a fully automated and simplified column-switching procedure to allow for the easy and automated determination of radioligands and their metabolites in up to 5 mL of plasma. The system has been used with conventional UV and coincidence radiation detectors, as well as with a single quadrupole mass spectrometer.

  12. Qualitative and quantitative analysis of the diuretic component ergone in Polyporus umbellatus by HPLC with fluorescence detection and HPLC-APCI-MS/MS.

    PubMed

    Zhao, Ying-Yong; Zhao, Ye; Zhang, Yong-Min; Lin, Rui-Chao; Sun, Wen-Ji

    2009-06-01

    Polyporus umbellatus is a widely used anti-aldosteronic diuretic in Traditional Chinese medicine (TCM). A new, sensitive and selective high-performance liquid chromatography-fluorescence detector (HPLC-FLD) and high-performance liquid chromatography-atmospheric pressure chemical ionization-mass spectrometry (HPLC-APCI-MS/MS) method for quantitative and qualitative determination of ergosta-4,6,8(14),22-tetraen-3-one(ergone), which is the main diuretic component, was provided for quality control of P. umbellatus crude drug. The ergone in the ethanolic extract of P. umbellatus was unambiguously characterized by HPLC-APCI, and further confirmed by comparing with a standard compound. The trace ergone was detected by the sensitive and selective HPLC-FLD. Linearity (r2 > 0.9998) and recoveries of low, medium and high concentration (100.5%, 100.2% and 100.4%) were consistent with the experimental criteria. The limit of detection (LOD) of ergone was around 0.2 microg/mL. Our results indicated that the content of ergone in P. umbellatus varied significantly from habitat to habitat with contents ranging from 2.13 +/- 0.02 to 59.17 +/- 0.05 microg/g. Comparison among HPLC-FLD and HPLC-UV or HPLC-APCI-MS/MS demonstrated that the HPLC-FLD and HPLC-APCI-MS/MS methods gave similar quantitative results for the selected herb samples, the HPLC-UV methods gave lower quantitative results than HPLC-FLD and HPLC-APCI-MS/MS methods. The established new HPLC-FLD method has the advantages of being rapid, simple, selective and sensitive, and could be used for the routine analysis of P. umbellatus crude drug.

  13. 78 FR 25840 - Installed Systems and Equipment for Use by the Flightcrew

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-05-03

    ... certified aircraft (examples include updating avionics systems, engines, drag reduction, interior... aircraft systems, equipment, and the aircraft itself, so that they understand the situation better. Active...-1175; Amdt. No. 25-138] RIN 2120-AJ83 Installed Systems and Equipment for Use by the Flightcrew AGENCY...

  14. The equipment access software for a distributed UNIX-based accelerator control system

    NASA Astrophysics Data System (ADS)

    Trofimov, Nikolai; Zelepoukine, Serguei; Zharkov, Eugeny; Charrue, Pierre; Gareyte, Claire; Poirier, Hervé

    1994-12-01

    This paper presents a generic equipment access software package for a distributed control system using computers with UNIX or UNIX-like operating systems. The package consists of three main components, an application Equipment Access Library, Message Handler and Equipment Data Base. An application task, which may run in any computer in the network, sends requests to access equipment through Equipment Library calls. The basic request is in the form Equipment-Action-Data and is routed via a remote procedure call to the computer to which the given equipment is connected. In this computer the request is received by the Message Handler. According to the type of the equipment connection, the Message Handler either passes the request to the specific process software in the same computer or forwards it to a lower level network of equipment controllers using MIL1553B, GPIB, RS232 or BITBUS communication. The answer is then returned to the calling application. Descriptive information required for request routing and processing is stored in the real-time Equipment Data Base. The package has been written to be portable and is currently available on DEC Ultrix, LynxOS, HPUX, XENIX, OS-9 and Apollo domain.

  15. Advances in HPLC-ICP-MS interface techniques for metal speciation

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Hill, S.J.

    The relentless demand for lower detection limits is increasingly coupled to the requirement for elemental speciation. This is particularly true in environmental and clinical fields where total levels are often insufficient for mobility and toxicity studies. This demand for both qualitative and quantitative data on the individual species present in complex samples has led to the development of various interfaces to couple some form of chromatography, usually gas chromatography (GC) or high performance liquid chromatography (HPLC) to an element specific detector. Today inductively coupled plasma-mass spectrometry is often employed since it offers excellent detection limits, element specific information (including isotopicmore » data) and the potential for multi-element studies. Ms presentation will concentrate on HPLC couplings although the advantages and disadvantages of both GC and HPLC couplings to ICP-MS will be discussed. Particular attention will be given to the optimization of both the chromatography and detection systems. Details will be presented of several successful HPLC interface designs and ways of facilitating high levels of a range of organic solvents (e.g. methanol and THF) in the HPLC mobile phase will be highlighted. The advantages of using a sheath gas and practical ways of achieving this will also be discussed. Finally the use of isotope dilution analysis in conjunction with HPLC-ICP-MS will be outlined. In all cases the impact of using the most appropriate approach will be demonstrated using both environmental and clinical samples.« less

  16. 47 CFR 80.231 - Technical Requirements for Class B Automatic Identification System (AIS) equipment.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... Identification System (AIS) equipment. 80.231 Section 80.231 Telecommunication FEDERAL COMMUNICATIONS COMMISSION... § 80.231 Technical Requirements for Class B Automatic Identification System (AIS) equipment. (a) Class B Automatic Identification System (AIS) equipment must meet the technical requirements of IEC 62287...

  17. 47 CFR 80.231 - Technical Requirements for Class B Automatic Identification System (AIS) equipment.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... Identification System (AIS) equipment. 80.231 Section 80.231 Telecommunication FEDERAL COMMUNICATIONS COMMISSION... § 80.231 Technical Requirements for Class B Automatic Identification System (AIS) equipment. (a) Class B Automatic Identification System (AIS) equipment must meet the technical requirements of IEC 62287...

  18. 47 CFR 80.231 - Technical Requirements for Class B Automatic Identification System (AIS) equipment.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... Identification System (AIS) equipment. 80.231 Section 80.231 Telecommunication FEDERAL COMMUNICATIONS COMMISSION... § 80.231 Technical Requirements for Class B Automatic Identification System (AIS) equipment. (a) Class B Automatic Identification System (AIS) equipment must meet the technical requirements of IEC 62287...

  19. Collaboration Mechanism for Equipment Instruction of Multiple Energy Systems

    NASA Astrophysics Data System (ADS)

    Wang, Dong; Wang, Tuo; Wang, Qi; Zhang, Zhao; Zhao, Mingyu; Wang, Yinghui

    2018-01-01

    When multiple energy systems execute optimization instructions simultaneously, and the same equipment is Shared, the instruction conflict may occur. Aiming at the above problems, taking into account the control objectives of each system, the characteristics of different systems, such as comprehensive clean energy, energy efficiency, and peak filling, etc., designed the instruction coordination mechanism for the daemon. This mechanism mainly acts on the main station of the system, and form a final optimization instruction. For some specific scenarios, the collaboration mechanism of unlocking the terminal is supplemented. The mechanism determines the specific execution instructions based on the arrival time of the instruction. Finally, the experiment in Tianjin eco-city shows that this algorithm can meet the instruction and collaboration requirements of multi-energy systems, and ensure the safe operation of the equipment.

  20. Firefighters Integrated Response Equipment System

    NASA Technical Reports Server (NTRS)

    Kaplan, H.; Abeles, F.

    1978-01-01

    The Firefighters Integrated Response Equipment System (Project FIRES) is a joint National Fire Prevention and Control Administration (NFPCA)/National Aeronautics and Space Administration (NASA) program for the development of an 'ultimate' firefighter's protective ensemble. The overall aim of Project FIRES is to improve firefighter protection against hazards, such as heat, flame, smoke, toxic fumes, moisture, impact penetration, and electricity and, at the same time, improve firefighter performance by increasing maneuverability, lowering weight, and improving human engineering design of his protective ensemble.

  1. Facility Systems, Ground Support Systems, and Ground Support Equipment General Design Requirements

    NASA Technical Reports Server (NTRS)

    Thaxton, Eric A.

    2014-01-01

    KSC-DE-512-SM establishes overall requirements and best design practices to be used at the John F. Kennedy Space Center (KSC) for the development of ground systems (GS) in support of operations at launch, landing, and retrieval sites. These requirements apply to the design and development of hardware and software for ground support equipment (GSE), ground support systems (GSS), and facility ground support systems (F-GSS) used to support the KSC mission for transportation, receiving, handling, assembly, test, checkout, servicing, and launch of space vehicles and payloads and selected flight hardware items for retrieval. This standards manual supplements NASA-STD-5005 by including KSC-site-specific and local environment requirements. These requirements and practices are optional for equipment used at manufacturing, development, and test sites.

  2. Electronic Equipment Maintenance Training (EEMT) System: System Definition Phase.

    ERIC Educational Resources Information Center

    Pine, S. M.; And Others

    The second in a series of four reports on the Electronic Equipment Maintenance Training (EEMT) project, this document summarizes the system definition phase of the EEMT program. The purpose of this phase of the project was to define a comprehensive set of functions and training requirements that the EEMT must satisfy within the training mission of…

  3. Comparative evaluation of ICP sample introduction systems to be used in the metabolite profiling of chlorine-containing pharmaceuticals via HPLC-ICP-MS.

    PubMed

    Klencsár, Balázs; Sánchez, Carlos; Balcaen, Lieve; Todolí, José; Lynen, Frederic; Vanhaecke, Frank

    2018-05-10

    A systematic evaluation of four different ICP sample introduction systems to be used in the context of metabolite profiling of chlorine-containing pharmaceuticals via HPLC-ICP-MS was carried out using diclofenac and its major metabolite, 4'-hydroxy-diclofenac, as model compounds. The strict requirements for GMP validation of chromatographic methods in the pharmaceutical industry were adhered to in this context. The final aim of this investigation is an extension of the applicability and validatability of HPLC-ICP-MS in the field of pharmaceutical R&D. Five different gradient programmes were tested while the baseline peak width (w b ), peak capacity (P), USP tailing factor (A s ) and USP signal-to-noise ratio (USP S/N) were determined as major indicators of the chromatographic performance and the values obtained were compared to the corresponding FDA recommendations (if applicable). Four different ICP-MS sample introductions systems were investigated involving two units typically working at higher flow rates (∼1.0 mL min -1 ) and another two systems working at lower flow rates (∼0.1 mL min -1 ). Optimal conditions with potential for applicability under GMP conditions were found at a mobile phase flow rate of 1.0 mL min -1 by using a pneumatic micro-flow LC nebulizer mounted onto a Peltier-cooled cyclonic spray chamber cooled to -1 °C for sample introduction. Under these conditions, HPLC-ICP-MS provided a chromatographic performance similar to that of HPLC with UV detection. The peak shape (USP tailing factor = 1.1-1.4) was significantly improved compared to that obtained with the Peltier-cooled Scott-type spray chamber. Two alternative sample introduction systems - a POINT ® and a High-Temperature Torch-Integrated Sample Introduction System (hTISIS) - were also tested at a flow rate of 0.1 mL min -1 using a chromatographic column with 1.0 mm ID. Although these systems allowed the peak shape to be improved compared to that obtained with

  4. Equipment management risk rating system based on engineering endpoints.

    PubMed

    James, P J

    1999-01-01

    The equipment management risk ratings system outlined here offers two significant departures from current practice: risk classifications are based on intrinsic device risks, and the risk rating system is based on engineering endpoints. Intrinsic device risks are categorized as physical, clinical and technical, and these flow from the incoming equipment assessment process. Engineering risk management is based on verification of engineering endpoints such as clinical measurements or energy delivery. This practice eliminates the ambiguity associated with ranking risk in terms of physiologic and higher-level outcome endpoints such as no significant hazards, low significance, injury, or mortality.

  5. Integrated software system for improving medical equipment management.

    PubMed

    Bliznakov, Z; Pappous, G; Bliznakova, K; Pallikarakis, N

    2003-01-01

    The evolution of biomedical technology has led to an extraordinary use of medical devices in health care delivery. During the last decade, clinical engineering departments (CEDs) turned toward computerization and application of specific software systems for medical equipment management in order to improve their services and monitor outcomes. Recently, much emphasis has been given to patient safety. Through its Medical Device Directives, the European Union has required all member nations to use a vigilance system to prevent the reoccurrence of adverse events that could lead to injuries or death of patients or personnel as a result of equipment malfunction or improper use. The World Health Organization also has made this issue a high priority and has prepared a number of actions and recommendations. In the present workplace, a new integrated, Windows-oriented system is proposed, addressing all tasks of CEDs but also offering a global approach to their management needs, including vigilance. The system architecture is based on a star model, consisting of a central core module and peripheral units. Its development has been based on the integration of 3 software modules, each one addressing specific predefined tasks. The main features of this system include equipment acquisition and replacement management, inventory archiving and monitoring, follow up on scheduled maintenance, corrective maintenance, user training, data analysis, and reports. It also incorporates vigilance monitoring and information exchange for adverse events, together with a specific application for quality-control procedures. The system offers clinical engineers the ability to monitor and evaluate the quality and cost-effectiveness of the service provided by means of quality and cost indicators. Particular emphasis has been placed on the use of harmonized standards with regard to medical device nomenclature and classification. The system's practical applications have been demonstrated through a pilot

  6. HPLC: Early and Recent Perspectives.

    ERIC Educational Resources Information Center

    Karger, Barry L.

    1997-01-01

    Provides a perspective on what it was like in the early days of high-performance liquid chromatography (HPLC) and several of the key developments. Focuses on the advances in HPLC generally, and more specifically for the biological sciences, that were necessary for the method to reach the preeminent stage of today. Contains 20 references. (JRH)

  7. Spectroscopic characterization and quantitative determination of atorvastatin calcium impurities by novel HPLC method

    NASA Astrophysics Data System (ADS)

    Gupta, Lokesh Kumar

    2012-11-01

    Seven process related impurities were identified by LC-MS in the atorvastatin calcium drug substance. These impurities were identified by LC-MS. The structure of impurities was confirmed by modern spectroscopic techniques like 1H NMR and IR and physicochemical studies conducted by using synthesized authentic reference compounds. The synthesized reference samples of the impurity compounds were used for the quantitative HPLC determination. These impurities were detected by newly developed gradient, reverse phase high performance liquid chromatographic (HPLC) method. The system suitability of HPLC analysis established the validity of the separation. The analytical method was validated according to International Conference of Harmonization (ICH) with respect to specificity, precision, accuracy, linearity, robustness and stability of analytical solutions to demonstrate the power of newly developed HPLC method.

  8. Polychlorinated Biphenyls (PCBs) in Transit System Electrical Equipment

    DOT National Transportation Integrated Search

    1984-05-01

    This report presents the legislative history and current regulatory requirement concerning the continued use of Polychlorinated Biphenyls (PCBs) in transit system electrical equipment. Recent rule-making promulgated by the Environmental Protection Ag...

  9. Comparative HPLC/ESI-MS and HPLC/DAD study of different populations of cultivated, wild and commercial Gentiana lutea L.

    PubMed

    Mustafa, Ahmed M; Caprioli, Giovanni; Ricciutelli, Massimo; Maggi, Filippo; Marín, Rosa; Vittori, Sauro; Sagratini, Gianni

    2015-05-01

    The root of Gentiana lutea L., famous for its bitter properties, is often used in alcoholic bitter beverages, food products and traditional medicine to stimulate the appetite and improve digestion. This study presents a new, fast, and accurate HPLC method using HPLC/ESI-MS and HPLC/DAD for simultaneous analysis of iridoids (loganic acid), secoiridoids (gentiopicroside, sweroside, swertiamarin, amarogentin) and xanthones (isogentisin) in different populations of G.lutea L., cultivated in the Monti Sibillini National Park, obtained wild there, or purchased commercially. Comparison of HPLC/ESI-MS and HPLC/DAD indicated that HPLC/ESI-MS is more sensitive, reliable and selective. Analysis of twenty samples showed that gentiopicroside is the most dominant compound (1.85-3.97%), followed by loganic acid (0.11-1.30%), isogentisin (0.03-0.48%), sweroside (0.05-0.35%), swertiamarin (0.08-0.30%), and amarogentin (0.01-0.07%). The results confirmed the high quality of the G.lutea cultivated in the Monti Sibillini National Park. Copyright © 2014 Elsevier Ltd. All rights reserved.

  10. Study of rat hypothalamic proteome by HPLC/ESI ion trap and HPLC/ESI-Q-TOF MS.

    PubMed

    Iqbal, Javed; Li, Wang; Ullah, Kaleem; Hasan, Murtaza; Linna, Guo; Awan, Umer; Zhang, Yongqian; Batool, Sajida; Qing, Hong; Deng, Yulin

    2013-08-01

    The proteomic profile of hypothalamus, a key organ of CNS, is explored here by employing two widely used MS techniques, i.e. HPLC/ESI-ion trap and HPLC/ESI-quadrupole-TOF MS. Strong cation exchange is used for the fractionation of peptides and protein search engine MASCOT is employed for data query. One hundred and thirty six proteins with 10 973 peptides were identified by HPLC/ESI-ion trap MS, while 140 proteins with 32 183 peptides were characterized by HPLC/ESI-quadrupole-TOF MS. Among the total 198 proteins identified in both experiments, 78 proteins were common in both sets of conditions. The rest of the 120 proteins were identified distinctly in both MS strategies, i.e. 58 unique proteins were found using the quadrupole-TOF while 62 were found with the HPLC/ESI-ion trap. Moreover, these proteins were classified into groups based on their functions performed in the body. Results presented here identified some important signal and cellular defense proteins inevitable for survival in stressed conditions. Additionally, it is also shown that any single MS strategy is not reliable for good results due to loss of data depending on sensitivity of the instrument used. © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

  11. Equipment Readiness Code Rule System (ERCRULES)

    DTIC Science & Technology

    1988-06-01

    Food inspection...CAA-SR-88-14 branch = ahs and equipment = ’Water quality set"’ or equipment = ’ Food inspection set’ then erc-assigned = [unitcriticalfacility,’ERC A...8217,𔃿.5.15𔃻. if not branch = qms and not branch = ahs and equipment = ’Water quality set’’ or equipment = ’ Food inspection set’ then

  12. The automatic control system and stand-by facilities of the TDMA-40 equipment

    NASA Astrophysics Data System (ADS)

    Gudenko, D. V.; Pankov, G. Kh.; Pauk, A. G.; Tsirlin, V. M.

    1980-10-01

    When a controlling station in a satellite communications system is out of order, a complex algorithm must be carried out for automatic operation of the stand-by equipment. A processor has been developed to perform this algorithm, as well as operations involving the stand-by facilities of the receiving-transmitting equipment of the station. The design principles and solutions to problems in developing the equipment for the monitoring and controlling systems are described. These systems are based on multistation access using time division multiplexing. Algorithms are presented for the operation of the synchronizing processor and the control processor of the equipment. The automatic control system and stand-by facilities make it possible to reduce the service personnel and to design an unattended station.

  13. HPLC for quality control of polyimides

    NASA Technical Reports Server (NTRS)

    Young, P. R.; Sykes, G. F.

    1979-01-01

    High Pressure Liquid Chromatography (HPLC) as a quality control tool for polyimide resins and prepregs are presented. A data base to help establish accept/reject criteria for these materials was developed. This work is intended to supplement, not replace, standard quality control tests normally conducted on incoming resins and prepregs. To help achieve these objectives, the HPLC separation of LARC-160 polyimide precursor resin was characterized. Room temperature resin aging effects were studied. Graphite reinforced composites made from fresh and aged resin were fabricated and tested to determine if changes observed by HPLC were significant.

  14. Simple HPLC method for detection of trace ephedrine and pseudoephedrine in high-purity methamphetamine.

    PubMed

    Makino, Yukiko

    2012-03-01

    A simple and sensitive HPLC technique was developed for the qualitative determination of ephedrine and pseudoephedrine (ephedrines), used as precursors of clandestine d-methamphetamine hydrochloride of high purity. Good separation of ephedrines from bulk d-methamphetamine was achieved, without any extraction or derivatization procedure on a CAPCELLPACK C18 MGII (250 × 4.6 mm) column. The mobile phase consisted of 50 mM KH2 PO4-acetonitrile (94:6 v/v %) using an isocratic pump system within 20 min for detecting two analytes. One run took about 50 min as it was necessary to wash out overloaded methamphetamine for column conditioning. The analytes were detected by UV absorbance measurement at 210 nm. A sample (20 mg) was simply dissolved in 1 mL of water, and a 50 μL aliquot of the solution was injected into the HPLC. The detection limits for ephedrine and pseudoephedrine in bulk d-methamphetamine were as low as 3 ppm each. This analytical separation technique made it possible to detect ephedrine and/or pseudoephedrine in seven samples of high-purity d-methamphetamine hydrochloride seized in Japan. The presence of trace ephedrines in illicit methamphetamine may strongly indicate a synthetic route via ephedrine in methamphetamine profiling. This method is simple and sensitive, requiring only commonly available equipment, and should be useful for high-purity methamphetamine profiling. Copyright © 2011 John Wiley & Sons, Ltd.

  15. An Investigation Into HPLC Data Quality Problems

    NASA Technical Reports Server (NTRS)

    Hooker, Stanford B.; VanHeukelem, Laurie

    2011-01-01

    This report summarizes the analyses and results produced by a five-member investigative team of Government, university, and industry experts, established by NASA HQ. The team examined data quality problems associated with high performance liquid chromatography (HPLC) analyses of pigment concentrations in seawater samples produced by the San Diego State University (SDSU) Center for Hydro-Optics and Remote Sensing (CHORS). This report shows CHORS did not validate the methods used before placing them into service to analyze field samples for NASA principal investigators (PIs), even though the HPLC literature contained easily accessible method validation procedures, and the importance of implementing them, more than a decade ago. In addition, there were so many sources of significant variance in the CHORS methodologies, that the HPLC system rarely operated within performance criteria capable of producing the requisite data quality. It is the recommendation of the investigative team to a) not correct the data, b) make all the data that was temporarily sequestered available for scientific use, and c) label the affected data with an appropriate warning, e.g., "These data are not validated and should not be used as the sole basis for a scientific result, conclusion, or hypothesis--independent corroborating evidence is required."

  16. Determination of the pseudoephedrine content in pharmaceutical formulations and in biological fluids using a microbore HPLC system interfaced to a microfluidic chemiluminescence detector.

    PubMed

    Kadavilpparampu, Afsal Mohammed; Al-Lawati, Haider A J; Suliman, FakhrEldin O; Al Kindy, Salma M Z

    2015-12-01

    A novel automated precolumn derivatization followed by separation using liquid chromatography for the determination of pseudoephedrine (PSE) by a microfluidic chemiluminescence detector has been developed. An on-line derivatization procedure was utilized by converting PSE into a highly light emitting species in a Ru(bipy)3(2+)-peroxydisulphate chemiluminescence (CL) system by derivatizing it with a 1.0 M formaldehyde solution. The derivatized analyte was directly injected into a microbore high-performance liquid chromatography (HPLC) system coupled to an on-chip chemiluminescence detector. The newly developed highly selective, sensitive and fast HPLC-CL method was validated and successfully applied for the analysis of PSE in pharmaceutical formulations and a human urine sample. The selectivity of the method is not only due to the HPLC separation but is also due to the highly selective detection principle of the Ru(bipy)3(2+)-peroxydisulphate CL system used. There was no interference observed from the common preservatives and excipients used in pharmaceutical preparations, which did not show any significant CL signal. The retention time of PSE was less than 3 min, and the detection limits and quantification limits were found to be 5.7 and 26.0 µg L(-1), respectively. Copyright © 2015 John Wiley & Sons, Ltd.

  17. Diagnosis - Using automatic test equipment and artificial intelligence expert systems

    NASA Astrophysics Data System (ADS)

    Ramsey, J. E., Jr.

    Three expert systems (ATEOPS, ATEFEXPERS, and ATEFATLAS), which were created to direct automatic test equipment (ATE), are reviewed. The purpose of the project was to develop an expert system to troubleshoot the converter-programmer power supply card for the F-15 aircraft and have that expert system direct the automatic test equipment. Each expert system uses a different knowledge base or inference engine, basing the testing on the circuit schematic, test requirements document, or ATLAS code. Implementing generalized modules allows the expert systems to be used for any different unit under test. Using converted ATLAS to LISP code allows the expert system to direct any ATE using ATLAS. The constraint propagated frame system allows for the expansion of control by creating the ATLAS code, checking the code for good software engineering techniques, directing the ATE, and changing the test sequence as needed (planning).

  18. Study on Earthquake Response of High Voltage Electrical Equipment Coupling System with Flexible Busbar

    NASA Astrophysics Data System (ADS)

    Liu, Chuncheng; Qu, Da; Wang, Chongyang; Lv, Chunlei; Li, Guoqiang

    2017-12-01

    With the rapid development of technology and society, all walks of life in China are becoming more and more dependent on power systems. When earthquake occurs, the electrical equipment of substation is prone to damage because of its own structural features, top-heavy, and brittleness of main body. At the same time, due to the complex coupling of the soft electrical connection of substation electrical equipment, the negative impact can not be estimated. In this paper, the finite element model of the coupling system of the single unit of high voltage electrical equipment with the connecting soft bus is established and the seismic response is analysed. The results showed that there is a significant difference between the simple analysis for the seismic response of electrical equipment monomer and the analytical results of electrical equipment systems, and the impact on different electrical equipment is different. It lays a foundation for the future development of seismic performance analysis of extra high voltage electrical equipment.

  19. Facility Systems, Ground Support Systems, and Ground Support Equipment General Design Requirements

    NASA Technical Reports Server (NTRS)

    Thaxton, Eric A.; Mathews, Roger E.

    2014-01-01

    This standard establishes requirements and guidance for design and fabrication of ground systems (GS) that includes: ground support equipment (GSE), ground support systems (GSS), and facility ground support systems (F GSS) to provide uniform methods and processes for design and development of robust, safe, reliable, maintainable, supportable, and cost-effective GS in support of space flight and institutional programs and projects.

  20. Trace analysis of pollutants in water using the photothermal interferometry as HPLC detector.

    PubMed

    Seidel, B S; Dübel, O; Faubel, W; Ache, H J

    1996-03-01

    A new procedure including high performance liquid chromatography in combination with photothermal interference spectroscopy as detection device (HPLC/PIS) has been proposed, optimized and its figures of merit for pesticide residue analysis are shown. The flowing sample under study is set in one arm of a Mach-Zehnder interferometer, and its refractive index is modulated by a periodically chopped continuous wave argon ion laser. As chopper, an acousto optical modulator has been introduced to switch the excitation laser beam between different lines (457 nm, 488 nm, 514 nm) simultaneously. Thus a multi component analysis can be realized either by using an HPLC-system in front of the PIS device or by a multi line Ar(+)-laser, directly. The limit of detection of the HPLC/PIS system reached 71 microg/l of the pesticide di-nitro-ortho-cresol (DNOC).

  1. 78 FR 19541 - Proposed Revision to Design of Structures, Components, Equipment and Systems

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-04-01

    ... NUCLEAR REGULATORY COMMISSION [NRC-2013-0041] Proposed Revision to Design of Structures, Components, Equipment and Systems AGENCY: Nuclear Regulatory Commission. ACTION: Standard review plan-draft..., ``Design of Structures, Components, Equipment, and Systems;'' and the request for comment on NUREG-0800...

  2. A Low Cost Remote Sensing System Using PC and Stereo Equipment

    NASA Technical Reports Server (NTRS)

    Campbell, Joel F.; Flood, Michael A.; Prasad, Narasimha S.; Hodson, Wade D.

    2011-01-01

    A system using a personal computer, speaker, and a microphone is used to detect objects, and make crude measurements using a carrier modulated by a pseudorandom noise (PN) code. This system can be constructed using a personal computer and audio equipment commonly found in the laboratory or at home, or more sophisticated equipment that can be purchased at reasonable cost. We demonstrate its value as an instructional tool for teaching concepts of remote sensing and digital signal processing.

  3. Designing efficient logging systems for northern hardwoods using equipment production capabilities and costs.

    Treesearch

    R.B. Gardner

    1966-01-01

    Describes a typical logging system used in the Lake and Northeastern States, discusses each step in the operation, and presents a simple method for designing and efficient logging system for such an operation. Points out that a system should always be built around the key piece of equipment, which is usually the skidder. Specific equipment types and their production...

  4. Screening and Analysis of the Marker Components in Ganoderma lucidum by HPLC and HPLC-MSn with the Aid of Chemometrics.

    PubMed

    Wu, Lingfang; Liang, Wenyi; Chen, Wenjing; Li, Shi; Cui, Yaping; Qi, Qi; Zhang, Lanzhen

    2017-04-06

    Ganoderma triterpenes (GTs) are the major secondary metabolites of Ganoderma lucidum , which is a popularly used traditional Chinese medicine for complementary cancer therapy. The present study was to establish a fingerprint evaluation system based on Similarity Analysis (SA), Cluster Analysis (CA) and Principal Component Analysis (PCA) for the identification and quality control of G. lucidum . Fifteen samples from the Chinese provinces of Hainan, Neimeng, Shangdong, Jilin, Anhui, Henan, Yunnan, Guangxi and Fujian were analyzed by HPLC-PAD and HPLC-MS n . Forty-seven compounds were detected by HPLC, of which forty-two compounds were tentatively identified by comparing their retention times and mass spectrometry data with that of reference compounds and reviewing the literature. Ganoderic acid B, 3,7,15-trihydroxy-11,23-dioxolanost-8,16-dien-26-oic acid, lucidenic acid A, ganoderic acid G, and 3,7-oxo-12-acetylganoderic acid DM were deemed to be the marker compounds to distinguish the samples with different quality according to both CA and PCA. This study provides helpful chemical information for further research on the anti-tumor activity and mechanism of action of G. lucidum . The results proved that fingerprints combined with chemometrics are a simple, rapid and effective method for the quality control of G. lucidum .

  5. A framework of medical equipment management system for in-house clinical engineering department.

    PubMed

    Chien, Chia-Hung; Huang, Yi-You; Chong, Fok-Ching

    2010-01-01

    Medical equipment management is an important issue for safety and cost in modern hospital operation. In addition, the use of an efficient information system effectively promotes the managing performance. In this study, we designed a framework of medical equipment management system used for in-house clinical engineering department. The system was web-based, and it integrated clinical engineering and hospital information system components. Through related information application, it efficiently improved the operation management of medical devices immediately and continuously. This system has run in the National Taiwan University Hospital. The results showed only few examples in the error analysis of medical equipment by the maintenance sub-system. The information can be used to improve work quality, to reduce the maintenance cost, and to promote the safety of medical device used in patients and clinical staffs.

  6. Determination of Tinopal CBS-X in rice papers and rice noodles using HPLC with fluorescence detection and LC-MS/MS.

    PubMed

    Ko, Kyung Yuk; Lee, Chae A; Choi, Jae Chon; Kim, Meehye

    2014-01-01

    To date there have been no reports of methods to determine Tinopal CBS-X. We developed a rapid and simple method to determine the Tinopal CBS-X content in rice noodles and rice papers using HPLC equipped with fluorescence detection. Heating the rice noodles and rice papers to 80°C after adding 75% methanol solution induced the release of Tinopal CBS-X from processed rice products. Tinopal CBS-X was separated using an isocratic mobile phase comprising 50% acetonitrile/water containing 0.4% tetrabutyl ammonium hydrogen sulphate at pH 8.0. The samples suspected to be positive by HPLC analysis were then confirmed by LC-MS/MS analysis. This study also investigated the Tinopal CBS-X content of three rice noodle products and two rice papers. The limits of quantification for rice papers and rice noodles were 1.58 and 1.51 µg kg(-1), respectively, and their correlation curves showed good linearity with r(2) ≥ 0.9997 and ≥ 0.9998, respectively. Moreover, rice papers had recoveries of 70.3-83.3% with precision ranging from 5.0% to 7.9%, whereas rice noodles had slightly lower recoveries of 63.4-78.7% and precisions of 8.5-11.5%. Only one rice noodle product contained Tinopal CBS-X, at around 2.1 mg kg(-1), whereas it was not detected in four other samples. Consequently, Tinopal CBS-X from rice noodles and rice papers can be successfully detected using the developed pre-treatment and ion-pairing HPLC system coupled with fluorescence detection.

  7. 46 CFR 176.816 - Miscellaneous systems and equipment.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 100 GROSS TONS) INSPECTION AND CERTIFICATION Material Inspections § 176.816 Miscellaneous systems and equipment. At each initial and subsequent inspection for certification the owner or managing operator shall be prepared to test and make available for inspection all items in the ship's outfit, such as ground...

  8. [Hospital clinical engineer orientation and function in the maintenance system of hospital medical equipment].

    PubMed

    Li, Bin; Zheng, Yunxin; He, Dehua; Jiang, Ruiyao; Chen, Ying; Jing, Wei

    2012-03-01

    The quantity of medical equipment in hospital rise quickly recent year. It provides the comprehensive support to the clinical service. The maintenance of medical equipment becomes more important than before. It is necessary to study on the orientation and function of clinical engineer in medical equipment maintenance system. Refer to three grade health care system, the community doctors which is called General practitioner, play an important role as the gatekeeper of health care system to triage and cost control. The paper suggests that hospital clinical engineer should play similar role as the gatekeeper of medical equipment maintenance system which composed by hospital clinical engineer, manufacture engineer and third party engineer. The hospital clinical engineer should be responsible of guard a pass of medical equipment maintenance quality and cost control. As the gatekeeper, hospital clinical engineer should take the responsibility of "General engineer" and pay more attention to safety and health of medical equipment. The responsibility description and future transition? development of clinical engineer as "General Engineer" is discussed. More attention should be recommended to the team building of hospital clinical engineer as "General Engineer".

  9. 40 CFR 141.561 - What happens if my system's turbidity monitoring equipment fails?

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 40 Protection of Environment 24 2013-07-01 2013-07-01 false What happens if my system's turbidity monitoring equipment fails? 141.561 Section 141.561 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY... happens if my system's turbidity monitoring equipment fails? If there is a failure in the continuous...

  10. 40 CFR 141.561 - What happens if my system's turbidity monitoring equipment fails?

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 40 Protection of Environment 23 2011-07-01 2011-07-01 false What happens if my system's turbidity monitoring equipment fails? 141.561 Section 141.561 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY... happens if my system's turbidity monitoring equipment fails? If there is a failure in the continuous...

  11. 40 CFR 141.561 - What happens if my system's turbidity monitoring equipment fails?

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 40 Protection of Environment 24 2012-07-01 2012-07-01 false What happens if my system's turbidity monitoring equipment fails? 141.561 Section 141.561 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY... happens if my system's turbidity monitoring equipment fails? If there is a failure in the continuous...

  12. 40 CFR 141.561 - What happens if my system's turbidity monitoring equipment fails?

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 40 Protection of Environment 23 2014-07-01 2014-07-01 false What happens if my system's turbidity monitoring equipment fails? 141.561 Section 141.561 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY... happens if my system's turbidity monitoring equipment fails? If there is a failure in the continuous...

  13. Aircraft interrogation and display system: A ground support equipment for digital flight systems

    NASA Technical Reports Server (NTRS)

    Glover, R. D.

    1982-01-01

    A microprocessor-based general purpose ground support equipment for electronic systems was developed. The hardware and software are designed to permit diverse applications in support of aircraft flight systems and simulation facilities. The implementation of the hardware, the structure of the software, describes the application of the system to an ongoing research aircraft project are described.

  14. Determination of sulfonamides and trimethoprim using high temperature HPLC with simultaneous temperature and solvent gradient.

    PubMed

    Giegold, Sascha; Teutenberg, Thorsten; Tuerk, Jochen; Kiffmeyer, Thekla; Wenclawiak, Bernd

    2008-10-01

    A fast HPLC method for the analysis of eight selected sulfonamides (SA) and trimethoprim has been developed with the use of high temperature HPLC. The separation could be achieved in less than 1.5 min on a 50 mm sub 2 microm column with simultaneous solvent and temperature gradient programming. Due to the lower viscosity of the mobile phase and the increased mass transfer at higher temperatures, the separation could be performed on a conventional HPLC system obtaining peak widths at half height between 0.6 and 1.3 s.

  15. Research on the energy and ecological efficiency of mechanical equipment remanufacturing systems

    NASA Astrophysics Data System (ADS)

    Shi, Junli; Cheng, Jinshi; Ma, Qinyi; Wang, Yajun

    2017-08-01

    According to the characteristics of mechanical equipment remanufacturing system, the dynamic performance of energy consumption and emission is explored, the equipment energy efficiency and emission analysis model is established firstly, and then energy and ecological efficiency analysis method of the remanufacturing system is put forward, at last, the energy and ecological efficiency of WD615.87 automotive diesel engine remanufacturing system as an example is analyzed, the way of energy efficiency improvementnt and environmental friendly mechanism of remanufacturing process is put forward.

  16. Repeatability Assessment by ISO 11843-7 in Quantitative HPLC for Herbal Medicines.

    PubMed

    Chen, Liangmian; Kotani, Akira; Hakamata, Hideki; Tsutsumi, Risa; Hayashi, Yuzuru; Wang, Zhimin; Kusu, Fumiyo

    2015-01-01

    We have proposed an assessment methods to estimate the measurement relative standard deviation (RSD) of chromatographic peaks in quantitative HPLC for herbal medicines by the methodology of ISO 11843 Part 7 (ISO 11843-7:2012), which provides detection limits stochastically. In quantitative HPLC with UV detection (HPLC-UV) of Scutellaria Radix for the determination of baicalin, the measurement RSD of baicalin by ISO 11843-7:2012 stochastically was within a 95% confidence interval of the statistically obtained RSD by repetitive measurements (n = 6). Thus, our findings show that it is applicable for estimating of the repeatability of HPLC-UV for determining baicalin without repeated measurements. In addition, the allowable limit of the "System repeatability" in "Liquid Chromatography" regulated in a pharmacopoeia can be obtained by the present assessment method. Moreover, the present assessment method was also successfully applied to estimate the measurement RSDs of quantitative three-channel liquid chromatography with electrochemical detection (LC-3ECD) of Chrysanthemi Flos for determining caffeoylquinic acids and flavonoids. By the present repeatability assessment method, reliable measurement RSD was obtained stochastically, and the experimental time was remarkably reduced.

  17. Research on multi - channel interactive virtual assembly system for power equipment under the “VR+” era

    NASA Astrophysics Data System (ADS)

    Ren, Yilong; Duan, Xitong; Wu, Lei; He, Jin; Xu, Wu

    2017-06-01

    With the development of the “VR+” era, the traditional virtual assembly system of power equipment has been unable to satisfy our growing needs. In this paper, based on the analysis of the traditional virtual assembly system of electric power equipment and the application of VR technology in the virtual assembly system of electric power equipment in our country, this paper puts forward the scheme of establishing the virtual assembly system of power equipment: At first, we should obtain the information of power equipment, then we should using OpenGL and multi texture technology to build 3D solid graphics library. After the completion of three-dimensional modeling, we can use the dynamic link library DLL package three-dimensional solid graphics generation program to realize the modularization of power equipment model library and power equipment model library generated hidden algorithm. After the establishment of 3D power equipment model database, we set up the virtual assembly system of 3D power equipment to separate the assembly operation of the power equipment from the space. At the same time, aiming at the deficiency of the traditional gesture recognition algorithm, we propose a gesture recognition algorithm based on improved PSO algorithm for BP neural network data glove. Finally, the virtual assembly system of power equipment can really achieve multi-channel interaction function.

  18. Aircraft skin cooling system for thermal management of onboard high power electronic equipment

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Hashemi, A.; Dyson, E.

    1996-12-31

    Integration of high-power electronic devices into existing aircraft, while minimizing the impact of additional heat load on the environmental control system of the aircraft, requires innovative approaches. One such approach is to reject heat through the aircraft skin by use of internal skin ducts with enhanced surfaces. This approach requires a system level consideration of the effect of cooling ducts, inlets and outlets on the performance of the electronic equipment and effectiveness of the heat rejection system. This paper describes the development of a system-level model to evaluate the performance of electronic equipment in an aircraft cabin and heat rejectionmore » through the skin. In this model, the outer surface of the fuselage is treated as a heat exchanger. Hot air from an equipment exhaust plenum is drawn into a series of baffled ducts within the fuselage support structure, where the heat is rejected, and then recirculated into the cabin. The cooler air form the cabin is then drawn into the electronic equipment. The aircraft air conditioning unit is also modeled to provide chilled air directly into the cabin. In addition, this paper describes a series of tests which were performed to verify the model assumptions for heat dissipation from and air flow through the equipment. The tests were performed using the actual electronic equipment in a representative cabin configuration. Results indicate very good agreement between the analytical calculations for the design point and model predictions.« less

  19. Extraction and identification of flavonoids from parsley extracts by HPLC analysis

    NASA Astrophysics Data System (ADS)

    Stan, M.; Soran, M. L.; Varodi, C.; Lung, I.

    2012-02-01

    Flavonoids are phenolic compounds isolated from a wide variety of plants, and are valuable for their multiple properties, including antioxidant and antimicrobial activities. In the present work, parsley (Petroselinum crispum L.) extracts were obtained by three different extraction techniques: maceration, ultrasonic-assisted and microwave-assisted solvent extractions. The extractions were performed with ethanol-water mixtures in various ratios. From these extracts, flavonoids like the flavones apigenin and luteolin, and the flavonols quercetin and kaempferol were identified using an HPLC Shimadzu apparatus equipped with PDA and MS detectors. The separation method involved a gradient step. The mobile phase consisted of two solvents: acetonitrile and distilled water with 0.1% formic acid. The separation was performed on a RP-C18 column.

  20. Comparison of adsorption coefficient (K[sub oc]) for soils and HPLC retention factors of aromatic hydrocarbons using a chemically immobilized humic acid column in RP-HPLC

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Szabo, G.; Bulman, R.A.

    The determination of soil adsorption coefficients (K[sub oc]) via HPLC capacity factors (k[prime]) has been studied, including the effect of column type and mobile phase composition on the correlation between log K[sub oc] and log k[prime]. K[sub oc] values obtained by procedures other than HPLC correlate well with HPLC capacity factors determined on a chemically immobilized humic acid stationary phase, and it is suggested that this phase is a better model for the sorption onto soil or sediment than the octadecyl-, phenyl- and ethylsilica phases. By using log k[prime][sub w] a theoretical capacity factor has been obtained by extrapolation ofmore » the retention data in a binary solvent system to pure aqueous eluent. There is a better correlation between log K[sub oc] and log k[prime][sub w] than the correlation between log K[sub oc] and log k[prime].« less

  1. Comparison of UHPLC and HPLC in Benzodiazepines Analysis of Postmortem Samples

    PubMed Central

    Behnoush, Behnam; Sheikhazadi, Ardeshir; Bazmi, Elham; Fattahi, Akbar; Sheikhazadi, Elham; Saberi Anary, Seyed Hossein

    2015-01-01

    Abstract The aim of this study was to compare system efficiency and analysis duration regarding the solvent consumption and system maintenance in high-pressure liquid chromatography (HPLC) and ultra high-pressure liquid chromatography (UHPLC). In a case–control study, standard solutions of 7 benzodiazepines (BZs) and 73 biological samples such as urine, tissue, stomach content, and bile that screened positive for BZs were analyzed by HPLC and UHPLC in laboratory of forensic toxicology during 2012 to 2013. HPLC analysis was performed using a Knauer by 100-5 C-18 column (250 mm × 4.6 mm) and Knauer photodiode array detector (PAD). UHPLC analysis was performed using Knauer PAD detector with cooling autosampler and Eurospher II 100-3 C-18 column (100 mm × 3 mm) and also 2 pumps. The mean retention time, standard deviation, flow rate, and repeatability of analytical results were compared by using 2 methods. Routine runtimes in HPLC and UHPLC took 40 and 15 minutes, respectively. Changes in mobile phase composition of the 2 methods were not required. Flow rate and solvent consumption in UHPLC decreased. Diazepam and flurazepam were detected more frequently in biological samples. In UHPLC, small particle size and short length of column cause effective separation of BZs in a very short time. Reduced flow rate, solvent consumption, and injection volume cause more efficiency and less analysis costs. Thus, in the detection of BZs, UHPLC is an accurate, sensitive, and fast method with less cost of analysis. PMID:25860209

  2. Study of phenformin metabolism in rat liver microsomes by HPLC, CE and on-line HPLC-electrospray ionization mass spectrometry.

    PubMed

    Llambias, E B; Luo, J

    1996-01-01

    Methods for the analysis of phenformin and its metabolite by high-performance liquid chromatography (HPLC), capillary electrophoresis (CE) and high-performance liquid chromatography-electrospray ionization mass spectrometry (HPLC-ESIMS) are developed. The effects of pH, buffer concentration and proportion of organic modifier on the retention of the compounds in HPLC have been studied. The optimum condition was used for the separation and identification of phenformin and its metabolite in microsomal metabolism by HPLC-ESIMS. A simple CE method is also described for the separation of these compounds. Optimum incubation conditions and cofactor requirements for the formation of 4-hydroxyphenformin by microsomal preparations of rat liver were determined. A linear response in the formation of product was found with increasing concentrations of protein and up to 15 min incubation. High concentrations of phenformin inhibited its metabolite formation, and K(m) was 4 microM.

  3. 47 CFR 80.275 - Technical Requirements for Class A Automatic Identification System (AIS) equipment.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... Compulsory Ships § 80.275 Technical Requirements for Class A Automatic Identification System (AIS) equipment. (a) Prior to submitting a certification application for a Class A AIS device, the following... Identification System (AIS) equipment. 80.275 Section 80.275 Telecommunication FEDERAL COMMUNICATIONS COMMISSION...

  4. 47 CFR 80.275 - Technical Requirements for Class A Automatic Identification System (AIS) equipment.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... Compulsory Ships § 80.275 Technical Requirements for Class A Automatic Identification System (AIS) equipment. (a) Prior to submitting a certification application for a Class A AIS device, the following... Identification System (AIS) equipment. 80.275 Section 80.275 Telecommunication FEDERAL COMMUNICATIONS COMMISSION...

  5. 47 CFR 80.275 - Technical Requirements for Class A Automatic Identification System (AIS) equipment.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... Compulsory Ships § 80.275 Technical Requirements for Class A Automatic Identification System (AIS) equipment. (a) Prior to submitting a certification application for a Class A AIS device, the following... Identification System (AIS) equipment. 80.275 Section 80.275 Telecommunication FEDERAL COMMUNICATIONS COMMISSION...

  6. 47 CFR 80.275 - Technical Requirements for Class A Automatic Identification System (AIS) equipment.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... Compulsory Ships § 80.275 Technical Requirements for Class A Automatic Identification System (AIS) equipment. (a) Prior to submitting a certification application for a Class A AIS device, the following... Identification System (AIS) equipment. 80.275 Section 80.275 Telecommunication FEDERAL COMMUNICATIONS COMMISSION...

  7. Determination of CMPO using HPLC -UV

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Gracy Elias; Gary S. Groenewold; Bruce J. Mincher

    Octyl(phenyl)-N,N-diisobutylcarbamoylmethylphosphine oxide (CMPO) is an extractant proposed for selective separation of radionuclide metals from used nuclear fuel solutions using solvent extraction. Radiolysis reactions can degrade CMPO and reduce separation performance and hence methods for measuring concentration of CMPO and identifying degradation products are needed. A novel high performance liquid chromatography (HPLC) method employing ultraviolet detection (UV) was developed to detect and quantitate CMPO in dodecane. Some radiolysis products in gamma and alpha irradiated CMPO solutions were identified using HPLC/electrospray ionization-mass spectrometry (ESI-MS). Validation data indicated that the HPLC-UV method for CMPO determination provided good linearity, sensitivity, procedure accuracy and systemmore » precision. CMPO-nitric acid complexes were also identified, that account for the apparent loss of CMPO in acidic environment, independent of irradiation.« less

  8. Fingerprint of Hedyotis diffusa Willd. by HPLC-MS.

    PubMed

    Yang, Ting; Yang, Yi-Hua; Yang, Ju-Yun; Chen, Ben-Mei; Duan, Ju-Ping; Yu, Shu-Yi; Ouyang, Hong-Tao; Cheng, Jun-Ping; Chen, Yu-Xiang

    2008-01-01

    A HPLC-MS fingerprint method has been developed based on the consistent chromatographic features of the major chemical constituents among 10 batches of Hedyotis diffusa Willd. Chromatographic separation was conducted on a Hypersil-Keystone Hypurity C(18) column using methanol:water:acetic acid as the mobile phase. Major compounds, including oleanolic acid, ursolic acid and ferulic acid, were analysed by HPLC-MS. Their analysis was ascertained by comparison with data derived from the standard compounds. The HPLC-MS fingerprint was successfully applied to analyse and differentiate samples from different geographical origins, or processing methods. H. diffusa was well distinguished from Hedyotis chrysotricha by HPLC-MS. Therefore the establishment of fingerprint of H. diffusa is critical in assessing and controlling its overall quality.

  9. Uninterruptible power systems and other power protection equipment for electronic health care systems.

    PubMed

    Massey, J K

    1979-01-01

    The increasing usage of electronic instruments in health care systems invariably leads to some level of dependence on them. In order to maximize the utility of these tools a high degree of reliability is essential. Many of the failures being experienced in systems where electronic instruments are being utilized may be attributed not to a failure of the instrument itself but rather to the poor quality of the commercial power to which they are attached. In order to reduce the effects of power fluctuations and outages, some type of power protection equipment must be installed between the commercial power system and the instrument. This article discusses the types of "electronic noise" present on commercial power lines and the various types of equipment used to reduce its effect on electronic instrumentation. In general, the Uninterruptible Power System (UPS) is shown to be the most effective power buffering element for a health care environment. General terminology associated with specifications of a UPS is defined in the article and attached appendix.

  10. Aqueous Reversed-Phase HPLC/FT-IR Using Diffuse Reflectance Detections

    NASA Astrophysics Data System (ADS)

    Kalasinsky, Victor F.; Pai, T. H.; Kenton, R. C.; Kalasinsky, Kathryn S.

    1989-12-01

    Solvent-elimination HPLC/FT-IR has become a viable combination of two important techniques, and we have been developing a system which is adaptable to both normal and reversed-phase liquid chromatography. The interface involves the deposition of HPLC eluites onto a KCI-laden train with subsequent analysis via diffuse reflectance spectroscopy, and with minor modifications, the system can be used with microbore and analytical columns. With aqueous solvents, the water is converted to methanol and acetone in a post-column reaction with 2,2-dimethoxypropane before the eluites are deposited. A number of different samples have been used to demonstrate the interface and its flexibility. Steroids, analgesics, and other pharmaceutical preparations have been separated with reverse-phase solvents and identified by their infrared spectra. For some of the compounds studied, different infrared spectra of a given compound have been found to exhibit intensity variations, which arise from different crystalline states. The differences can be concentration dependent and may be useful in obtaining semi-quantitative information from the infrared spectra. Applications involving both gradient elution and isocratic separations have been successful. The former provides the same advantages for HPLC/FT-IR as one finds in conventional HPLC. More recent work has been applied to the use of buffers such as those frequently used in bioanalytical separations. In trying to simplify the post-column reaction with water, we have immobilized dehydration reagents onto silica particles and packed these materials into a column which is inserted in-line after the analytical column. Of the reagents utilized to date, 3,3-dimethoxypropyltrimethoxysilane has been found to perform most efficiently. It has advantages over the simpler reagents because it can be regenerated in the reaction column. Results and the efficiency of the dehydration process and its relation to the type of reagent and its coverage will be

  11. Continuous Flow Liquid Microjunction Surface Sampling Probe Connected On-line with HPLC/MS for Spatially Resolved Analysis of Small Molecules and Proteins

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Van Berkel, Gary J; Kertesz, Vilmos

    RATIONALE: A continuous flow liquid microjunction surface sampling probe extracts soluble material from surfaces for direct ionization and detection by MS. Demonstrated here is the on-line coupling of such a probe with HPLC/MS enabling extraction, separation and detection of small molecules and proteins from surfaces in a spatially resolved (~0.5 mm diameter spots) manner. Methods: A continuous flow liquid microjunction surface sampling probe was connected to a 6-port, 2-position valve for extract collection and injection to an HPLC column. A QTRAP 5500 hybrid triple quadrupole linear ion trap equipped with a Turbo V ion source operated in positive ESI modemore » was used for all experiments. System operation was tested with extraction, separation and detection of propranolol and associated metabolites from drug dosed tissues and proteins from dried sheep blood spots on paper. Results: Confirmed in the tissue were the parent drug and two different hydroxypropranolol glucuronides. The mass spectrometric response for these compounds from different locations in the liver showed an increase with increasing extraction time (5, 20 and 40 s extractions). For on-line separation and detection/identification of extracted proteins from dried sheep blood spots, two major protein peaks dominated the chromatogram and could be correlated with the expected masses for the hemoglobin and chains. Conclusions: Spatially resolved sampling, separation, and detection of small molecules and proteins from surfaces can be accomplished using a continuous flow liquid microjunction surface sampling probe coupled on-line with HPLC/MS detection.« less

  12. Study of multi-functional precision optical measuring system for large scale equipment

    NASA Astrophysics Data System (ADS)

    Jiang, Wei; Lao, Dabao; Zhou, Weihu; Zhang, Wenying; Jiang, Xingjian; Wang, Yongxi

    2017-10-01

    The effective application of high performance measurement technology can greatly improve the large-scale equipment manufacturing ability. Therefore, the geometric parameters measurement, such as size, attitude and position, requires the measurement system with high precision, multi-function, portability and other characteristics. However, the existing measuring instruments, such as laser tracker, total station, photogrammetry system, mostly has single function, station moving and other shortcomings. Laser tracker needs to work with cooperative target, but it can hardly meet the requirement of measurement in extreme environment. Total station is mainly used for outdoor surveying and mapping, it is hard to achieve the demand of accuracy in industrial measurement. Photogrammetry system can achieve a wide range of multi-point measurement, but the measuring range is limited and need to repeatedly move station. The paper presents a non-contact opto-electronic measuring instrument, not only it can work by scanning the measurement path but also measuring the cooperative target by tracking measurement. The system is based on some key technologies, such as absolute distance measurement, two-dimensional angle measurement, automatically target recognition and accurate aiming, precision control, assembly of complex mechanical system and multi-functional 3D visualization software. Among them, the absolute distance measurement module ensures measurement with high accuracy, and the twodimensional angle measuring module provides precision angle measurement. The system is suitable for the case of noncontact measurement of large-scale equipment, it can ensure the quality and performance of large-scale equipment throughout the process of manufacturing and improve the manufacturing ability of large-scale and high-end equipment.

  13. Determination of the Antibiotic Oxytetracycline in Commercial Milk by Solid-Phase Extraction: A High-Performance Liquid Chromatography (HPLC) Experiment for Quantitative Instrumental Analysis

    ERIC Educational Resources Information Center

    Mei-Ratliff, Yuan

    2012-01-01

    Trace levels of oxytetracylcine spiked into commercial milk samples are extracted, cleaned up, and preconcentrated using a C[subscript 18] solid-phase extraction column. The extract is then analyzed by a high-performance liquid chromatography (HPLC) instrument equipped with a UV detector and a C[subscript 18] column (150 mm x 4.6 mm x 3.5 [mu]m).…

  14. Acceptance Equipment System Data Acquisition and Processing Utility

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Fakhro, Rowan

    2015-02-01

    My internship at Sandia National Laboratories took place in the Department of Sensors and Embedded Systems, which is tasked with, among many things, the non-destructive testing of thermal batteries. The Acceptance Equipment System (AES) is a flexible rack system designed to electrically test thermal batteries individually for internal defects before they are stored in the battery stock pile. Aside from individual testing, data acquired by the AES is used for many things including trending and catching outliers within the tolerance levels of a particular battery type, allowing for the development of more refined acceptance requirements and testing procedures.

  15. Isolation and structural characterization of a novel sibutramine analogue, chlorosipentramine, in a slimming dietary supplement, by using HPLC-PDA, LC-Q-TOF/MS, FT-IR, and NMR.

    PubMed

    Yun, Jisuk; Shin, Kye Jung; Choi, Jangduck; Jo, Cheon-Ho

    2018-05-01

    A novel sibutramine analogue was detected in a slimming formula by high performance liquid chromatography with a photo diode detector array (HPLC-PDA). The unknown compound exhibited an ultraviolet (UV) spectrum that was similar to that of chlorosibutramine, despite having a different HPLC retention time. Further analysis of the slimming formula by LC-quadrupole time-of-flight mass spectrometry (LC-Q-TOF/MS) showed that the unknown compound had the formula C 18 H 27 Cl 2 N. To elucidate the structure of this new sibutramine analogue, the target compound in the slimming formula was isolated on a preparative-LC system equipped with a PDA. After analysis by fourier transform infrared (FT-IR) and nuclear magnetic resonance (NMR) spectroscopy, the unknown compound was identified as a sibutramine analogue in which the iso-butyl group on the side chain is replaced with an iso-pentyl group. This new sibutramine analogue was identified to be 1-(1-(3,4-dichlorophenyl)cyclobutyl)-N,N,4-trimethylpentan-1-amine and has been named as chlorosipentramine. Copyright © 2018 Elsevier B.V. All rights reserved.

  16. Monitoring of multiple bacteriocins through a developed dual extraction protocol and comparison of HPLC-DAD with turbidometry as their quantification system.

    PubMed

    Katharopoulos, Efstathios; Touloupi, Katerina; Touraki, Maria

    2016-08-01

    The present study describes the development of a simple and efficient screening system that allows identification and quantification of nine bacteriocins produced by Lactococcus lactis. Cell-free L. lactis extracts presented a broad spectrum of antibacterial activity, including Gram-negative bacteria, Gram-positive bacteria, and fungi. The characterization of their sensitivity to pH, and heat, showed that the extracts retained their antibacterial activity at extreme pH values and in a wide temperature range. The loss of antibacterial activity following treatment of the extracts with lipase or protease suggests a lipoproteinaceous nature of the produced antimicrobials. The extracts were subjected to a purification protocol that employs a two phase extraction using ammonium sulfate precipitation and organic solvent precipitation, followed by ion exchange chromatography, solid phase extraction and HPLC. In the nine fractions that presented antimicrobial activity, bacteriocins were quantified by the turbidometric method using a standard curve of nisin and by the HPLC method with nisin as the external standard, with both methods producing comparable results. Turbidometry appears to be unique in the qualitative determination of bacteriocins but the only method suitable to both separate and quantify the bacteriocins providing increased sensitivity, accuracy, and precision is HPLC. Copyright © 2016 Elsevier B.V. All rights reserved.

  17. Extraction, Separation, and Identification of Phenolic Compounds in Virgin Olive Oil by HPLC-DAD and HPLC-MS

    PubMed Central

    Tasioula-Margari, Maria; Tsabolatidou, Eleftheria

    2015-01-01

    The aim of this study was to evaluate the recovery of individual phenolic compounds extracted from virgin olive oil (VOO), from different Greek olive varieties. Sufficient recoveries (90%) of all individual phenolic compounds were obtained using methanol as an extraction solvent, acetonitrile for residue solubilization, and two washing steps with hexane. Moreover, in order to elucidate structural characteristics of phenolic compounds in VOO, high performance liquid chromatography with a diode array detector (HPLC-DAD) at 280 and 340 nm and HPLC coupled to electrospray ionization mass spectrometry (HPLC-ESI-MS) in the negative-ion mode were performed. The most abundant phenolic compounds were oleuropein derivatives with m/z 319 and 377 and ligstroside derivatives with m/z 303, 361. Lignans, such as 1-acetoxypinoresinol and pinoresinol were also present in substantial quantities in the phenolic fraction. However, pinoresinol was co-eluted with dialdehydic form of ligstroside aglycone (DAFLA) and it was not possible to be quantified separately. The phenolic extracts, obtained from different VOO samples, yielded similar HPLC profiles. Differences, however, were observed in the last part of the chromatogram, corresponding to isomers of the aldehydic form of ligstroside aglycone. Oxidized phenolic products, originating from secoiridoids, were also detected. PMID:26783843

  18. Maintenance Training Equipment: Design Specification Based on Instructional System Development. Revision

    DTIC Science & Technology

    1984-12-01

    model pr, vides a method for communicating a specific training equipment design to the procurement office after A"~- ISD ana lysis has est~blished a...maintenance trainer has been identified. The model provides a method by which a training equipment design can be communicated to the System Project Office...ensure * ase of development of procurement specifications and consistency between different documented designs. A completed application of this maodel

  19. HPLC-Chip/MS Technology in Proteomic Profiling

    NASA Astrophysics Data System (ADS)

    Vollmer, Martin; van de Goor, Tom

    HPLC-chip/MS is a novel nanoflow analytical technology conducted on a microfabricated chip that allows for highly efficient HPLC separation and superior sensitive MS detection of complex proteomic mixtures. This is possible through on-chip preconcentration and separation with fluidic connection made automatically in a leak-tight fashion. Minimum precolumn and postcolumn peak dispersion and uncompromised ease of use result in compounds eluting in bands of only a few nanoliters. The chip is fabricated out of bio-inert polyimide-containing channels and integrated chip structures, such as an electrospray emitter, columns, and frits manufactured by laser ablation technology. Meanwhile, a variety of HPLC-chips differing in design and stationary phase are commercially available, which provide a comprehensive solution for applications in proteomics, glycomics, biomarker, and pharmaceutical discovery. The HPLC-chip can also be easily integrated into a multidimensional separation workflow where different orthogonal separation techniques are combined to solve a highly complex separation problems. In this chapter, we describe in detail the methodological chip usage and functionality and its application in the elucidation of the protein profile of human nucleoli.

  20. [Development of a medical equipment support information system based on PDF portable document].

    PubMed

    Cheng, Jiangbo; Wang, Weidong

    2010-07-01

    According to the organizational structure and management system of the hospital medical engineering support, integrate medical engineering support workflow to ensure the medical engineering data effectively, accurately and comprehensively collected and kept in electronic archives. Analyse workflow of the medical, equipment support work and record all work processes by the portable electronic document. Using XML middleware technology and SQL Server database, complete process management, data calculation, submission, storage and other functions. The practical application shows that the medical equipment support information system optimizes the existing work process, standardized and digital, automatic and efficient orderly and controllable. The medical equipment support information system based on portable electronic document can effectively optimize and improve hospital medical engineering support work, improve performance, reduce costs, and provide full and accurate digital data

  1. MS-BWME: A Wireless Real-Time Monitoring System for Brine Well Mining Equipment

    PubMed Central

    Xiao, Xinqing; Zhu, Tianyu; Qi, Lin; Moga, Liliana Mihaela; Zhang, Xiaoshuan

    2014-01-01

    This paper describes a wireless real-time monitoring system (MS-BWME) to monitor the running state of pumps equipment in brine well mining and prevent potential failures that may produce unexpected interruptions with severe consequences. MS-BWME consists of two units: the ZigBee Wireless Sensors Network (WSN) unit and the real-time remote monitoring unit. MS-BWME was implemented and tested in sampled brine wells mining in Qinghai Province and four kinds of indicators were selected to evaluate the performance of the MS-BWME, i.e., sensor calibration, the system's real-time data reception, Received Signal Strength Indicator (RSSI) and sensor node lifetime. The results show that MS-BWME can accurately judge the running state of the pump equipment by acquiring and transmitting the real-time voltage and electric current data of the equipment from the spot and provide real-time decision support aid to help workers overhaul the equipment in a timely manner and resolve failures that might produce unexpected production down-time. The MS-BWME can also be extended to a wide range of equipment monitoring applications. PMID:25340455

  2. Towards the development of a rapid, portable, surface enhanced Raman spectroscopy based cleaning verification system for the drug nelarabine.

    PubMed

    Corrigan, Damion K; Salton, Neale A; Preston, Chris; Piletsky, Sergey

    2010-09-01

    Cleaning verification is a scientific and economic problem for the pharmaceutical industry. A large amount of potential manufacturing time is lost to the process of cleaning verification. This involves the analysis of residues on spoiled manufacturing equipment, with high-performance liquid chromatography (HPLC) being the predominantly employed analytical technique. The aim of this study was to develop a portable cleaning verification system for nelarabine using surface enhanced Raman spectroscopy (SERS). SERS was conducted using a portable Raman spectrometer and a commercially available SERS substrate to develop a rapid and portable cleaning verification system for nelarabine. Samples of standard solutions and swab extracts were deposited onto the SERS active surfaces, allowed to dry and then subjected to spectroscopic analysis. Nelarabine was amenable to analysis by SERS and the necessary levels of sensitivity were achievable. It is possible to use this technology for a semi-quantitative limits test. Replicate precision, however, was poor due to the heterogeneous drying pattern of nelarabine on the SERS active surface. Understanding and improving the drying process in order to produce a consistent SERS signal for quantitative analysis is desirable. This work shows the potential application of SERS for cleaning verification analysis. SERS may not replace HPLC as the definitive analytical technique, but it could be used in conjunction with HPLC so that swabbing is only carried out once the portable SERS equipment has demonstrated that the manufacturing equipment is below the threshold contamination level.

  3. Design of efficient and simple interface testing equipment for opto-electric tracking system

    NASA Astrophysics Data System (ADS)

    Liu, Qiong; Deng, Chao; Tian, Jing; Mao, Yao

    2016-10-01

    Interface testing for opto-electric tracking system is one important work to assure system running performance, aiming to verify the design result of every electronic interface matching the communication protocols or not, by different levels. Opto-electric tracking system nowadays is more complicated, composed of many functional units. Usually, interface testing is executed between units manufactured completely, highly depending on unit design and manufacture progress as well as relative people. As a result, it always takes days or weeks, inefficiently. To solve the problem, this paper promotes an efficient and simple interface testing equipment for opto-electric tracking system, consisting of optional interface circuit card, processor and test program. The hardware cards provide matched hardware interface(s), easily offered from hardware engineer. Automatic code generation technique is imported, providing adaption to new communication protocols. Automatic acquiring items, automatic constructing code architecture and automatic encoding are used to form a new program quickly with adaption. After simple steps, a standard customized new interface testing equipment with matching test program and interface(s) is ready for a waiting-test system in minutes. The efficient and simple interface testing equipment for opto-electric tracking system has worked for many opto-electric tracking system to test entire or part interfaces, reducing test time from days to hours, greatly improving test efficiency, with high software quality and stability, without manual coding. Used as a common tool, the efficient and simple interface testing equipment for opto-electric tracking system promoted by this paper has changed traditional interface testing method and created much higher efficiency.

  4. An intelligent healthcare management system: a new approach in work-order prioritization for medical equipment maintenance requests.

    PubMed

    Hamdi, Naser; Oweis, Rami; Abu Zraiq, Hamzeh; Abu Sammour, Denis

    2012-04-01

    The effective maintenance management of medical technology influences the quality of care delivered and the profitability of healthcare facilities. Medical equipment maintenance in Jordan lacks an objective prioritization system; consequently, the system is not sensitive to the impact of equipment downtime on patient morbidity and mortality. The current work presents a novel software system (EQUIMEDCOMP) that is designed to achieve valuable improvements in the maintenance management of medical technology. This work-order prioritization model sorts medical maintenance requests by calculating a priority index for each request. Model performance was assessed by utilizing maintenance requests from several Jordanian hospitals. The system proved highly efficient in minimizing equipment downtime based on healthcare delivery capacity, and, consequently, patient outcome. Additionally, a preventive maintenance optimization module and an equipment quality control system are incorporated. The system is, therefore, expected to improve the reliability of medical equipment and significantly improve safety and cost-efficiency.

  5. 43. View of CSMR room equipment locator and system checkout ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    43. View of CSMR room equipment locator and system checkout console for detection radars and rearward communication data links in transmitter building no. 102. - Clear Air Force Station, Ballistic Missile Early Warning System Site II, One mile west of mile marker 293.5 on Parks Highway, 5 miles southwest of Anderson, Anderson, Denali Borough, AK

  6. UV LED-based lightweight fixed-wavelength detector: for the development of a miniaturized high-performance liquid chromatography (HPLC) system (Erratum)

    NASA Astrophysics Data System (ADS)

    Guan, Wen Yin; Lee, Wei Yan; Liew, Mervyn Wing On; Tan, Soo Choon; Lim, Jit Kang

    2018-02-01

    miniaturized HPLC system are portability for onsite analysis, increased efficiency, lower solvent requirements and fully integrated separation and detection system.

  7. Design of free-space optical transmission system in computer tomography equipment

    NASA Astrophysics Data System (ADS)

    Liu, Min; Fu, Weiwei; Zhang, Tao

    2018-04-01

    Traditional computer tomography (CT) based on capacitive coupling cannot satisfy the high data rate transmission requirement. We design and experimentally demonstrate a free-space optical transmission system for CT equipment at a data rate of 10 Gb / s. Two interchangeable sections of 12 pieces of fiber with equal length is fabricated and tested by our designed laser phase distance measurement system. By locating the 12 collimators in the edge of the circle wheel evenly, the optical propagation characteristics for the 12 wired and wireless paths are similar, which can satisfy the requirement of high-speed CT transmission system. After bit error rate (BER) measurement in several conditions, the BER performances are below the value of 10 - 11, which has the potential in the future application scenario of CT equipment.

  8. [Study on HPLC fingerprint of Alpinia officinarum].

    PubMed

    Deng, Yi-Feng; Feng, Li-Na; Luo, Hui

    2011-09-01

    To establish the chromatography fingerprint of Alpinia officinarum by HPLC. An optimum HPLC conditions which were obtained under the assessment of LC-MS were as follows: Shim-pack VP-ODS column (2.0 mm x 250 mm, 5 microm), 0.1% HAc aqueous solution as phase A, 15% Acetonitrile: 40% Methanol: 45% Tetrafuran as phase B, the flow rate was 0.20 mL/min, column temperature was 35 degrees C and UV detector was set at 280 nm. The HPLC fingerprint of Alpinia officinarum was established, the consensus 10 peaks and their relative retention times along with the ranges of relative area were determined. The method is reliable and stable and can be used for the quality control and identification of Alpinia officinarum.

  9. Shuttle orbiter Ku-band radar/communications system design evaluation. Deliverable test equipment evaluation

    NASA Technical Reports Server (NTRS)

    Maronde, R. G.

    1980-01-01

    The Ku-band test equipment, known as the Deliverable System Test equipment (DSTE), is reviewed and evaluated. The DSTE is semiautomated and computer programs were generated for 14 communication mode tests and 17 radar mode tests. The 31 test modules provide a good cross section of tests with which to exercise the Ku-band system; however, it is very limited when being used to verify Ku-band system performance. More detailed test descriptions are needed, and a major area of concern is the DSTE sell-off procedure which is inadequate.

  10. An enhanced droplet-based liquid microjunction surface sampling system coupled with HPLC-ESI-MS/MS for spatially resolved analysis

    DOE PAGES

    Van Berkel, Gary J.; Weiskittel, Taylor M.; Kertesz, Vilmos

    2014-11-07

    Droplet-based liquid microjunction surface sampling coupled with high-performance liquid chromatography (HPLC)-electrospray ionization (ESI)-tandem mass spectrometry (MS/MS) for spatially resolved analysis provides the possibility of effective analysis of complex matrix samples and can provide a greater degree of chemical information from a single spot sample than is typically possible with a direct analysis of an extract. Described here is the setup and enhanced capabilities of a discrete droplet liquid microjunction surface sampling system employing a commercially available CTC PAL autosampler. The system enhancements include incorporation of a laser distance sensor enabling unattended analysis of samples and sample locations of dramatically disparatemore » height as well as reliably dispensing just 0.5 μL of extraction solvent to make the liquid junction to the surface, wherein the extraction spot size was confined to an area about 0.7 mm in diameter; software modifications improving the spatial resolution of sampling spot selection from 1.0 to 0.1 mm; use of an open bed tray system to accommodate samples as large as whole-body rat thin tissue sections; and custom sample/solvent holders that shorten sampling time to approximately 1 min per sample. Lastly, the merit of these new features was demonstrated by spatially resolved sampling, HPLC separation, and mass spectral detection of pharmaceuticals and metabolites from whole-body rat thin tissue sections and razor blade (“crude”) cut mouse tissue.« less

  11. An enhanced droplet-based liquid microjunction surface sampling system coupled with HPLC-ESI-MS/MS for spatially resolved analysis

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Van Berkel, Gary J.; Weiskittel, Taylor M.; Kertesz, Vilmos

    Droplet-based liquid microjunction surface sampling coupled with high-performance liquid chromatography (HPLC)-electrospray ionization (ESI)-tandem mass spectrometry (MS/MS) for spatially resolved analysis provides the possibility of effective analysis of complex matrix samples and can provide a greater degree of chemical information from a single spot sample than is typically possible with a direct analysis of an extract. Described here is the setup and enhanced capabilities of a discrete droplet liquid microjunction surface sampling system employing a commercially available CTC PAL autosampler. The system enhancements include incorporation of a laser distance sensor enabling unattended analysis of samples and sample locations of dramatically disparatemore » height as well as reliably dispensing just 0.5 μL of extraction solvent to make the liquid junction to the surface, wherein the extraction spot size was confined to an area about 0.7 mm in diameter; software modifications improving the spatial resolution of sampling spot selection from 1.0 to 0.1 mm; use of an open bed tray system to accommodate samples as large as whole-body rat thin tissue sections; and custom sample/solvent holders that shorten sampling time to approximately 1 min per sample. Lastly, the merit of these new features was demonstrated by spatially resolved sampling, HPLC separation, and mass spectral detection of pharmaceuticals and metabolites from whole-body rat thin tissue sections and razor blade (“crude”) cut mouse tissue.« less

  12. Fluorescent HPLC for the detection of specific protein oxidation carbonyls - α-aminoadipic and γ-glutamic semialdehydes - in meat systems.

    PubMed

    Utrera, Mariana; Morcuende, David; Rodríguez-Carpena, Javier-Germán; Estévez, Mario

    2011-12-01

    Precise methodologies for the routine analysis of particular protein carbonyls are required in order to progress in this topic of increasing interest. The present paper originally describes the application of an improved method for the detection of α-aminoadipic and γ-glutamic semialdehydes in a meat system by using a derivatization procedure with p-amino-benzoic acid (ABA) followed by fluorescent high-performance liquid chromatography (HPLC). The method development comprises i) the description of a simple HPLC program which allows the efficient separation of the ABA and the key standard compounds and ii) the optimization of the procedure for the preparation of a meat sample in order to maximize the fluorescent signal for both protein carbonyls. Furthermore, the suitability of this method is evaluated by applying the technique to porcine burger patties. The present procedure enables an accurate and relatively fast analysis of both semialdehydes in meat samples in which they could play an interesting role as reliable indicators of protein oxidation. Copyright © 2011 Elsevier Ltd. All rights reserved.

  13. Applications of HPLC/MS in the analysis of traditional Chinese medicines

    PubMed Central

    Li, Miao; Hou, Xiao-Fang; Zhang, Jie; Wang, Si-Cen; Fu, Qiang; He, Lang-Chong

    2012-01-01

    In China, traditional Chinese medicines (TCMs) have been used in clinical applications for thousands of years. The successful hyphenation of high-Performance liquid chromatography (HPLC) and mass spectrometry (MS) has been applied widely in TCMs and biological samples analysis. Undoubtedly, HPLC/MS technique has facilitated the understanding of the treatment mechanism of TCMs. We reviewed more than 350 published papers within the last 5 years on HPLC/MS in the analysis of TCMs. The present review focused on the applications of HPLC/MS in the component analysis, metabolites analysis, and pharmacokinetics of TCMs etc. 50% of the literature is related to the component analysis of TCMs, which show that this field is the most populär type of research. In the metabolites analysis, HPLC coupled with electrospray ionization quadrupole time-of-flight tandem mass spectrometry has been demonstrated to be the powerful tool for the characterization of structural features and fragmentation behavior patterns. This paper presented a brief overview of the applications of HPLC/MS in the analysis of TCMs. HPLC/MS in the fingerprint analysis is reviewed elsewhere. PMID:29403684

  14. DETAIL VIEW OF TESTING EQUIPMENT, REMOTE MANIPULATOR SYSTEM LAB, ROOM ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    DETAIL VIEW OF TESTING EQUIPMENT, REMOTE MANIPULATOR SYSTEM LAB, ROOM NO. 1N4, FACING SOUTHEAST - Cape Canaveral Air Force Station, Launch Complex 39, Vehicle Assembly Building, VAB Road, East of Kennedy Parkway North, Cape Canaveral, Brevard County, FL

  15. Analysis of sesquiterpene lactones, lignans, and flavonoids in wormwood (Artemisia absinthium L.) using high-performance liquid chromatography (HPLC)-mass spectrometry, reversed phase HPLC, and HPLC-solid phase extraction-nuclear magnetic resonance.

    PubMed

    Aberham, Anita; Cicek, Serhat Sezai; Schneider, Peter; Stuppner, Hermann

    2010-10-27

    Today, the medicinal use of wormwood (Artemisia absinthium) is enjoying a resurgence of popularity. This study presents a specific and validated high-performance liquid chromatography (HPLC)-diode array detection method for the simultaneous determination and quantification of bioactive compounds in wormwood and commercial preparations thereof. Five sesquiterpene lactones, two lignans, and a polymethoxylated flavonoid were baseline separated on RP-18 material, using a solvent gradient consisting of 0.085% (v/v) o-phosphoric acid and acetonitrile. The flow rate was 1.0 mL/min, and chromatograms were recorded at 205 nm. The stability of absinthin was tested exposing samples to light, moisture, and different temperatures. Methanolic and aqueous solutions of absinthin were found to be stable for up to 6 months. This was also the case when the solid compound was kept in the refrigerator at -35 °C. In contrast, the colorless needles, when stored at room temperature, turned yellow. Three degradation compounds (anabsin, anabsinthin, and the new dimer 3'-hydroxyanabsinthin) were identified by HPLC-mass spectrometry and HPLC-solid-phase extraction-nuclear magnetic resonance and quantified by the established HPLC method.

  16. Analysis of D3-,4-,5-phosphorylated phosphoinositides using HPLC.

    PubMed

    Munnik, Teun

    2013-01-01

    Detection of polyphosphoinositides (PPIs) is difficult due to their low chemical abundancy. This problem is further complicated by the fact that PPIs are present as various, distinct isomers, which are difficult, if not impossible, to separate by conventional thin layer chromatography (TLC) systems. PPIs in plants include PtdIns3P, PtdIns4P, PtdIns5P, PtdIns(3,5)P 2, and PtdIns(4,5)P 2. Here, a protocol is described analyzing plant PPIs using (32)P-orthophosphorus pre-labeled material. After extraction, lipids are deacylated and the resulting glycerophosphoinositol polyphosphates (GroPInsPs) separated by HPLC using a strong anion-exchange column and a shallow salt gradient. Alternatively, PPIs are first separated by TLC, the lipids reisolated, deacylated, and the GroPInsPs then separated by HPLC.

  17. 47 CFR 36.123 - Operator systems equipment-Category 1.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... apportioned on the basis of the relative processor real time (i.e., actual seconds) required to process TSPS... relative processor real time (i.e., actual seconds) for the entire TSPS complex. [52 FR 17229, May 6, 1987... 47 Telecommunication 2 2014-10-01 2014-10-01 false Operator systems equipment-Category 1. 36.123...

  18. 47 CFR 36.123 - Operator systems equipment-Category 1.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... apportioned on the basis of the relative processor real time (i.e., actual seconds) required to process TSPS... relative processor real time (i.e., actual seconds) for the entire TSPS complex. [52 FR 17229, May 6, 1987... 47 Telecommunication 2 2013-10-01 2013-10-01 false Operator systems equipment-Category 1. 36.123...

  19. 47 CFR 36.123 - Operator systems equipment-Category 1.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... apportioned on the basis of the relative processor real time (i.e., actual seconds) required to process TSPS... relative processor real time (i.e., actual seconds) for the entire TSPS complex. [52 FR 17229, May 6, 1987... 47 Telecommunication 2 2012-10-01 2012-10-01 false Operator systems equipment-Category 1. 36.123...

  20. 47 CFR 36.123 - Operator systems equipment-Category 1.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... apportioned on the basis of the relative processor real time (i.e., actual seconds) required to process TSPS... relative processor real time (i.e., actual seconds) for the entire TSPS complex. [52 FR 17229, May 6, 1987... 47 Telecommunication 2 2011-10-01 2011-10-01 false Operator systems equipment-Category 1. 36.123...

  1. 47 CFR 36.123 - Operator systems equipment-Category 1.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... apportioned on the basis of the relative processor real time (i.e., actual seconds) required to process TSPS... relative processor real time (i.e., actual seconds) for the entire TSPS complex. [52 FR 17229, May 6, 1987... 47 Telecommunication 2 2010-10-01 2010-10-01 false Operator systems equipment-Category 1. 36.123...

  2. 78 FR 15755 - Proposed Revision to Design of Structures, Components, Equipment and Systems; Correction

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-03-12

    ... NUCLEAR REGULATORY COMMISSION [NRC-2013-0041] Proposed Revision to Design of Structures, Components, Equipment and Systems; Correction AGENCY: Nuclear Regulatory Commission. ACTION: Standard review... for comments of the proposed revision in Chapter 3, ``Design of Structures, Components, Equipment, and...

  3. Separation of {sup 32}P-postlabeled DNA adducts of polycyclic aromatic hydrocarbons and nitrated polycyclic aromatic hydrocarbons by HPLC

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    King, L.C.; Gallagher, J.E.; Lewtas, J.

    The {sup 32}P-postlabeling assay, thin-layer chromatography, and reverse-phase high-pressure liquid chromatography (HPLC) were used to separate DNA adducts formed from 10 polycyclic aromatic hydrocarbons (PAHs) and 6 nitrated polycyclic aromatic hydrocarbons (NO{sub 2}-PAHs). The PAHs included benzo[j]fluoranthene, benzo[k]fluoranthene, indeno[1,2,3-cd]pyrene, benzo[a]pyrene, chrysene, 6-methylchrysene, 5-methylchrysene, and benz[a]anthracene. The NO{sub 2}-PAHs included 1-nitropyrene, 2-nitrofluoranthene, 3-nitrofluoranthene, 1,6-dinitropyrene, 1,3-dinitropyrene, and 1,8-dinitropyrene. Separation of seven of the major PAH-DNA adducts was achieved by an initial PAH HPLC gradient system. The major NO{sub 2}-PAH-DNA adducts were not all separated from each other using the initial PAH HPLC gradient but were clearly separated from the PAH-DNA adducts. Amore » second NO{sub 2}-PAH HPLC gradient system was developed to separate NO{sub 2}-PAH-DNA adducts following one-dimensional TLC and HPLC analysis. HPLC profiles of NO{sub 2}-PAH-DNA adducts were compared using both adduct enhancement versions of the {sup 32}P-postlabeling assay to evaluate the use of this technique on HPLC to screen for the presence of NO{sub 2}-PAH-DNA adducts. To demonstrate the application of these separation methods to a complex mixture of DNA adducts, the chromatographic mobilities of the {sup 32}P-postlabeled DNA adduct standards (PAHs and NO{sub 2}-PAHs) were compared with those produced by a complex mixture of polycyclic organic matter (POM) extracted from diesel emission particles. The diesel-derived adducts did not elute with the identical retention time of any of the PAH or NO{sub 2}-PAH standards used in this study. HPLC analyses of the NO{sub 2}-PAH-derived adducts (butanol extracted) revealed the presence of multiple DNA adducts.« less

  4. Lunar surface construction and assembly equipment study: Lunar Base Systems Study (LBSS) task 5.3

    NASA Technical Reports Server (NTRS)

    1988-01-01

    A set of construction and assembly tasks required on the lunar surface was developed, different concepts for equipment applicable to the tasks determined, and leading candidate systems identified for future conceptual design. Data on surface construction and assembly equipment systems are necessary to facilitate an integrated review of a complete lunar scenario.

  5. Simultaneous determination of carbonyls and NO2 in exhausts of heavy-duty diesel trucks and transit buses by HPLC following 2,4-dinitrophenylhydrazine cartridge collection.

    PubMed

    Tang, Shida; Graham, Lisa; Shen, Ling; Zhou, Xianliang; Lanni, Thomas

    2004-11-15

    A method combining 2,4-dinitrophenylhydrazine (DNPH) cartridge sampling and high-performance liquid chromatography (HPLC) analysis has been used for the measurement of carbonyl and NO2 emissions from heavy-duty diesel trucks and transit buses. The reaction of NO2 with DNPH allows for the simultaneous and unambiguous determination of NO2 and carbonyl concentrations in exhaust samples. The potential coelution of the NO2-DNPH derivative with the formaldehyde-DNPH derivative under certain chromatographic conditions was investigated. Successful separation of these two species was achieved allowing for simultaneous determination of carbonyls and NO2 in the exhaust samples collected from heavy-duty diesel (HDD) trucks and diesel, diesel/electric hybrid, diesel equipped with the continuously regenerating technology (CRT) particle traps, and compressed natural gas (CNG) transit buses tested over various drive cycles. Elevated NO2 emissions from CRT-equipped buses were observed. The NO2/NOx volume ratios for HDD trucks and transit buses are discussed. A comparison of the DNPH derivatization with HPLC/UV-visible detection method with a chemiluminescence analyzer method for NO2 measurement is presented for a limited number of diesel/CRT and CNG buses.

  6. Laboratory equipment maintenance: a critical bottleneck for strengthening health systems in sub-Saharan Africa?

    PubMed

    Fonjungo, Peter N; Kebede, Yenew; Messele, Tsehaynesh; Ayana, Gonfa; Tibesso, Gudeta; Abebe, Almaz; Nkengasong, John N; Kenyon, Thomas

    2012-02-01

    Properly functioning laboratory equipment is a critical component for strengthening health systems in developing countries. The laboratory can be an entry point to improve population health and care of individuals for targeted diseases - prevention, care, and treatment of TB, HIV/AIDS, and malaria, plus maternal and neonatal health - as well as those lacking specific attention and funding. We review the benefits and persistent challenges associated with sustaining laboratory equipment maintenance. We propose equipment management policies as well as a comprehensive equipment maintenance strategy that would involve equipment manufacturers and strengthen local capacity through pre-service training of biomedical engineers. Strong country leadership and commitment are needed to assure development and sustained implementation of policies and strategies for standardization of equipment, and regulation of its procurement, donation, disposal, and replacement.

  7. Design and implement of mobile equipment management system based on QRcode

    NASA Astrophysics Data System (ADS)

    Yu, Runze; Duan, Xiaohui; Jiao, Bingli

    2017-08-01

    A mobile equipment management system based on QRcode is proposed for remote and convenient device management. Unlike conventional systems, the system here makes managers accessible to real-time information with smart phones. Compared with the conventional method, which can only be operated with specific devices, this lightweight and efficient tele management mode is conducive to the asset management in multiple scenarios.

  8. Guidance, navigation, and control subsystem equipment selection algorithm using expert system methods

    NASA Technical Reports Server (NTRS)

    Allen, Cheryl L.

    1991-01-01

    Enhanced engineering tools can be obtained through the integration of expert system methodologies and existing design software. The application of these methodologies to the spacecraft design and cost model (SDCM) software provides an improved technique for the selection of hardware for unmanned spacecraft subsystem design. The knowledge engineering system (KES) expert system development tool was used to implement a smarter equipment section algorithm than that which is currently achievable through the use of a standard data base system. The guidance, navigation, and control subsystems of the SDCM software was chosen as the initial subsystem for implementation. The portions of the SDCM code which compute the selection criteria and constraints remain intact, and the expert system equipment selection algorithm is embedded within this existing code. The architecture of this new methodology is described and its implementation is reported. The project background and a brief overview of the expert system is described, and once the details of the design are characterized, an example of its implementation is demonstrated.

  9. Numerical system for monitoring pressurized equipment

    NASA Astrophysics Data System (ADS)

    Dobra, Remus; Pasculescu, Dragos; Boca, Maria Loredana; Moldovan, Lucian

    2016-12-01

    Electrical devices for operation in potentially explosive atmospheres are designed and built in accordance with European standard EN 50015: 1995 ex. the pressurized enclosure "p". The type of protector p, by using a protective gas in the housing is intended to prevent the formation of an explosive atmosphere within it, while maintaining an overpressure to the surrounding atmosphere and, where appropriate, by the use dilution. Research conducted for pressurized encapsulation aimed at developing new procedures for determining the parameters of pressurization to allow safe use of electrical appliances. Pressurization with compensation for losses allegedly maintaining overpressure inside the enclosure when the outlets are closed, is made by feeding protective gas in an amount sufficient to fully compensate for losses from the housing inevitable pressurized and its associated pipework. The conditions and necessary measures that are required for appliances and equipment with potential ignition of explosive atmospheres are detailed in the SR EN 50016/2000. For pressurized encapsulation protection mode, the electric equipment can be maintained safety by the overpressure created inside them and in the supply pipes with air. The paper presents a modern method to determine the parameters of the electric equipment with pressurization enclosures. For controlling of such equipment, a specific algorithm has been developed and laboratory tested.

  10. Information technology equipment cooling method

    DOEpatents

    Schultz, Mark D.

    2015-10-20

    According to one embodiment, a system for removing heat from a rack of information technology equipment may include a sidecar indoor air to liquid heat exchanger that cools air utilized by the rack of information technology equipment to cool the rack of information technology equipment. The system may also include a liquid to liquid heat exchanger and an outdoor heat exchanger. The system may further include configurable pathways to connect and control fluid flow through the sidecar heat exchanger, the liquid to liquid heat exchanger, the rack of information technology equipment, and the outdoor heat exchanger based upon ambient temperature and/or ambient humidity to remove heat generated by the rack of information technology equipment.

  11. Challenges in legislation, recycling system and technical system of waste electrical and electronic equipment in China.

    PubMed

    Zhang, Shengen; Ding, Yunji; Liu, Bo; Pan, De'an; Chang, Chein-chi; Volinsky, Alex A

    2015-11-01

    Waste electrical and electronic equipment (WEEE) has been one of the fastest growing waste streams worldwide. Effective and efficient management and treatment of WEEE has become a global problem. As one of the world's largest electronic products manufacturing and consumption countries, China plays a key role in the material life cycle of electrical and electronic equipment. Over the past 20 years, China has made a great effort to improve WEEE recycling. Centered on the legal, recycling and technical systems, this paper reviews the progresses of WEEE recycling in China. An integrated recycling system is proposed to realize WEEE high recycling rate for future WEEE recycling. Copyright © 2015 Elsevier Ltd. All rights reserved.

  12. Hyperbaric Chamber Equipment: A Consolidated Equipment List from Selected Multiplace Hyperbaric Facilities.

    DTIC Science & Technology

    1983-12-01

    carbon dioxide scrubbers , air conditioning, communications, lighting, and fire detecting and fire extinguishing systems. Medical support equipment was...10 14 Humidity...............................11 5. Hydrocarb on...........................11 B. Carbon Dioxide Scrubbers .....................11 C...and ancillary equipment included gas/vapor monitoring equipment, carbon dioxide scrubbers , air conditioning, communications, lighting, and fire

  13. 40 CFR 267.34 - When must personnel have access to communication equipment or an alarm system?

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... to an internal alarm or emergency communication device, either directly or through visual or voice... communication equipment or an alarm system? 267.34 Section 267.34 Protection of Environment ENVIRONMENTAL... have access to communication equipment or an alarm system? (a) Whenever hazardous waste is being poured...

  14. Analyses of procyanidins in foods using Diol phase HPLC

    USDA-ARS?s Scientific Manuscript database

    Separation of procyanidins using silica-based HPLC suffered from poor resolution for higher oligomers and low sensitivity due to the fluorescence quenching effects of methylene chloride in the mobile phase. Optimization of a published Diol-phase HPLC method resulted in near baseline separation for p...

  15. Optimising waste from electric and electronic equipment collection systems: a comparison of approaches in European countries.

    PubMed

    Friege, Henning; Oberdörfer, Michael; Günther, Marko

    2015-03-01

    The first European waste from electric and electronic equipment directive obliged the Member States to collect 4 kg of used devices per inhabitant and year. The target of the amended directive focuses on the ratio between the amount of waste from electric and electronic equipment collected and the mass of electric and electronic devices put on the market in the three foregoing years. The minimum collection target is 45% starting in 2016, being increased to 65% in 2019 or alternatively 85% of waste from electric and electronic equipment generated. Being aware of the new target, the question arises how Member States with 'best practice' organise their collection systems and how they enforce the parties in this playing field. Therefore the waste from electric and electronic equipment schemes of Sweden, Denmark, Switzerland, Germany and the Flemish region of Belgium were investigated focusing on the categories IT and telecommunications equipment, consumer equipment like audio systems and discharge lamps containing hazardous substances, e.g. mercury. The systems for waste from electric and electronic equipment collection in these countries vary considerably. Recycling yards turned out to be the backbone of waste from electric and electronic equipment collection in most countries studied. For discharge lamps, take-back by retailers seems to be more important. Sampling points like special containers in shopping centres, lidded waste bins and complementary return of used devices in all retail shops for electric equipment may serve as supplements. High transparency of collection and recycling efforts can encourage ambition among the concerned parties. Though the results from the study cannot be transferred in a simplistic manner, they serve as an indication for best practice methods for waste from electric and electronic equipment collection. © The Author(s) 2015.

  16. PBF (PER620) interior. System control racks, secondary control and equipment ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    PBF (PER-620) interior. System control racks, secondary control and equipment room. Date: May 2004. INEEL negative no. HD-41-6-2 - Idaho National Engineering Laboratory, SPERT-I & Power Burst Facility Area, Scoville, Butte County, ID

  17. Preparation and analysis of standardized waste samples for Controlled Ecological Life Support Systems (CELSS)

    NASA Technical Reports Server (NTRS)

    Carden, J. L.; Browner, R.

    1982-01-01

    The preparation and analysis of standardized waste samples for controlled ecological life support systems (CELSS) are considered. Analysis of samples from wet oxidation experiments, the development of ion chromatographic techniques utilizing conventional high pressure liquid chromatography (HPLC) equipment, and an investigation of techniques for interfacing an ion chromatograph (IC) with an inductively coupled plasma optical emission spectrometer (ICPOES) are discussed.

  18. G-index: A new metric to describe dynamic refractive index effects in HPLC absorbance detection.

    PubMed

    Kraiczek, Karsten G; Rozing, Gerard P; Zengerle, Roland

    2018-09-01

    High performance liquid chromatography (HPLC) with a solvent gradient and absorbance detection is one of the most widely used methods in analytical chemistry. The observed absorbance baseline is affected by the changes in the refractive index (RI) of the mobile phase. Near the limited of detection, this complicates peak quantitation. The general aspects of these RI-induced apparent absorbance effects are discussed. Two different detectors with fundamentally different optics and flow cell concepts, a variable-wavelength detector equipped with a conventional flow cell and a diode-array detector equipped with a liquid core waveguide flow cell, are compared with respect to their RI behavior. A simple method to separate static - partly unavoidable - RI effects from dynamic RI effects is presented. It is shown that the dynamic RI behavior of an absorbance detector can be well described using a single, relatively easy-to-determine metric called the G-index. The G-index is typically in the order of a few seconds and its sign depends on the optical flow cell concept. Copyright © 2018 Elsevier B.V. All rights reserved.

  19. Automating the Air Force Retail-Level Equipment Management Process: An Application of Microcomputer-Based Information Systems Techniques

    DTIC Science & Technology

    1988-09-01

    could use the assistance of a microcomputer-based management information system . However, adequate system design and development requires an in-depth...understanding of the Equipment Management Section and the environment in which it functions were asked and answered. Then, a management information system was...designed, developed, and tested. The management information system is called the Equipment Management Information System (EMIS).

  20. 47 CFR 80.275 - Technical Requirements for Class A Automatic Identification System (AIS) equipment.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 47 Telecommunication 5 2010-10-01 2010-10-01 false Technical Requirements for Class A Automatic Identification System (AIS) equipment. 80.275 Section 80.275 Telecommunication FEDERAL COMMUNICATIONS COMMISSION (CONTINUED) SAFETY AND SPECIAL RADIO SERVICES STATIONS IN THE MARITIME SERVICES Equipment Authorization for Compulsory Ships § 80.275...

  1. Retinoid quantification by HPLC/MS(n)

    NASA Technical Reports Server (NTRS)

    McCaffery, Peter; Evans, James; Koul, Omanand; Volpert, Amy; Reid, Kevin; Ullman, M. David

    2002-01-01

    Retinoic acid (RA) mediates most of the biological effects of vitamin A that are essential for vertebrate survival. It acts through binding to receptors that belong to the nuclear receptor transcription factor superfamily (Mangelsdorf et al. 1994). It is also a highly potent vertebrate teratogen. To determine the function and effects of endogenous and exogenous RA, it is important to have a highly specific, sensitive, accurate, and precise analytical procedure. Current analyses of RA and other retinoids are labor intensive, of poor sensitivity, have limited specificity, or require compatibility with RA reporter cell lines (Chen et al. 1995. BIOCHEM: Pharmacol. 50: 1257-1264; Creech Kraft et al. 1994. BIOCHEM: J. 301: 111-119; Lanvers et al. 1996. J. Chromatogr. B Biomed. Appl. 685: 233-240; Maden et al. 1998. DEVELOPMENT: 125: 4133-4144; Wagner et al. 1992. DEVELOPMENT: 116: 55-66). This paper describes an HPLC/mass spectrometry/mass spectrometry product ion scan (HPLC/MS(n)) procedure for the analysis of retinoids that employs atmospheric pressure chemical ionization MS. The retinoids are separated by normal-phase column chromatography with a linear hexane-isopropanol-dioxane gradient. Each retinoid is detected by a unique series of MS(n) functions set at optimal collision-induced dissociation energy (30% to 32%) for all MS(n) steps. The scan events are divided into three segments, based on HPLC elution order, to maximize the mass spectrometer duty cycle. The all-trans, 9-cis, and 13-cis RA isomers are separated, if desired, by an isocratic hexane-dioxane-isopropanol mobile phase. This paper describes an HPLC/MS(n) procedure possessing high sensitivity and specificity for retinoids.

  2. 46 CFR 28.405 - Hydraulic equipment.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... than four times the system maximum operating pressure. (c) Each hydraulic system must be equipped with... 46 Shipping 1 2013-10-01 2013-10-01 false Hydraulic equipment. 28.405 Section 28.405 Shipping... Operate With More Than 16 Individuals on Board § 28.405 Hydraulic equipment. (a) Each hydraulic system...

  3. 46 CFR 28.405 - Hydraulic equipment.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... than four times the system maximum operating pressure. (c) Each hydraulic system must be equipped with... 46 Shipping 1 2010-10-01 2010-10-01 false Hydraulic equipment. 28.405 Section 28.405 Shipping... Operate With More Than 16 Individuals on Board § 28.405 Hydraulic equipment. (a) Each hydraulic system...

  4. 46 CFR 28.405 - Hydraulic equipment.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... than four times the system maximum operating pressure. (c) Each hydraulic system must be equipped with... 46 Shipping 1 2014-10-01 2014-10-01 false Hydraulic equipment. 28.405 Section 28.405 Shipping... Operate With More Than 16 Individuals on Board § 28.405 Hydraulic equipment. (a) Each hydraulic system...

  5. 46 CFR 28.405 - Hydraulic equipment.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... than four times the system maximum operating pressure. (c) Each hydraulic system must be equipped with... 46 Shipping 1 2011-10-01 2011-10-01 false Hydraulic equipment. 28.405 Section 28.405 Shipping... Operate With More Than 16 Individuals on Board § 28.405 Hydraulic equipment. (a) Each hydraulic system...

  6. 46 CFR 28.405 - Hydraulic equipment.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... than four times the system maximum operating pressure. (c) Each hydraulic system must be equipped with... 46 Shipping 1 2012-10-01 2012-10-01 false Hydraulic equipment. 28.405 Section 28.405 Shipping... Operate With More Than 16 Individuals on Board § 28.405 Hydraulic equipment. (a) Each hydraulic system...

  7. Reverse-phase HPLC analysis of human alpha crystallin.

    PubMed

    Swamy, M S; Abraham, E C

    1991-03-01

    A rapid and highly sensitive reverse-phase HPLC (RP-HPLC) method was used to separate crystallin subunits from human alpha crystallin. Three distinct peaks were separated; by electrophoretic and immunological analyses the first and second peaks were identified as alpha B and alpha A respectively. On the other hand, peak 3 appeared to be a modified form of alpha crystallin. The ratio of alpha A and alpha B proteins was 3:1 in 1 day old lenses which gradually changed to 2:1 in 17 year old lenses and to 1:1 in the 50 and 82 year old whole lenses and 82 year old lens cortex, with a concomitant increase in the modified alpha, suggesting that alpha A subunits are relatively more involved in aggregation. Analysis of the 82 year old lens nucleus also supported this conclusion. The RP-HPLC analysis of the HMW aggregate fraction showed substantial enrichment of the modified alpha. The alpha A and alpha B subunits independently reassociated to form polymeric alpha crystallin whereas the modified alpha reassociated to form HMW aggregates as shown by molecular sieve HPLC. Hence it appears that the HMW aggregate peak was constituted by modified alpha crystallin. Only in the peak 3 material the 280 nm absorbance was about 2-fold higher than what was expected from the actual protein content. The data suggest that the changes induced by post-translational modifications may have some role in the formation of modified alpha. The present RP-HPLC method is useful in separating these modified alpha from the unmodified alpha A and alpha B subunits.

  8. 46 CFR 61.20-3 - Main and auxiliary machinery and associated equipment, including fluid control systems.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... control for the means of stopping machinery driving forced and induced draft fans, fuel oil transfer pumps..., including fluid control systems. 61.20-3 Section 61.20-3 Shipping COAST GUARD, DEPARTMENT OF HOMELAND... Equipment § 61.20-3 Main and auxiliary machinery and associated equipment, including fluid control systems...

  9. 46 CFR 61.20-3 - Main and auxiliary machinery and associated equipment, including fluid control systems.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... control for the means of stopping machinery driving forced and induced draft fans, fuel oil transfer pumps..., including fluid control systems. 61.20-3 Section 61.20-3 Shipping COAST GUARD, DEPARTMENT OF HOMELAND... Equipment § 61.20-3 Main and auxiliary machinery and associated equipment, including fluid control systems...

  10. 46 CFR 61.20-3 - Main and auxiliary machinery and associated equipment, including fluid control systems.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... control for the means of stopping machinery driving forced and induced draft fans, fuel oil transfer pumps..., including fluid control systems. 61.20-3 Section 61.20-3 Shipping COAST GUARD, DEPARTMENT OF HOMELAND... Equipment § 61.20-3 Main and auxiliary machinery and associated equipment, including fluid control systems...

  11. 46 CFR 61.20-3 - Main and auxiliary machinery and associated equipment, including fluid control systems.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... control for the means of stopping machinery driving forced and induced draft fans, fuel oil transfer pumps..., including fluid control systems. 61.20-3 Section 61.20-3 Shipping COAST GUARD, DEPARTMENT OF HOMELAND... Equipment § 61.20-3 Main and auxiliary machinery and associated equipment, including fluid control systems...

  12. Optimizing Chromatographic Separation: An Experiment Using an HPLC Simulator

    ERIC Educational Resources Information Center

    Shalliker, R. A.; Kayillo, S.; Dennis, G. R.

    2008-01-01

    Optimization of a chromatographic separation within the time constraints of a laboratory session is practically impossible. However, by employing a HPLC simulator, experiments can be designed that allow students to develop an appreciation of the complexities involved in optimization procedures. In the present exercise, a HPLC simulator from "JCE…

  13. REDEX - The ranging equipment diagnostic expert system

    NASA Technical Reports Server (NTRS)

    Luczak, Edward C.; Gopalakrishnan, K.; Zillig, David J.

    1989-01-01

    REDEX, an advanced prototype expert system that diagnoses hardware failures in the Ranging Equipment (RE) at NASA's Ground Network tracking stations is described. REDEX will help the RE technician identify faulty circuit cards or modules that must be replaced, and thereby reduce troubleshooting time. It features a highly graphical user interface that uses color block diagrams and layout diagrams to illustrate the location of a fault. A semantic network knowledge representation technique was used to model the design structure of the RE. A catalog of generic troubleshooting rules was compiled to represent heuristics that are applied in diagnosing electronic equipment. Specific troubleshooting rules were identified to represent additional diagnostic knowledge that is unique to the RE. Over 50 generic and 250 specific troubleshooting rules have been derived. REDEX is implemented in Prolog on an IBM PC AT-compatible workstation. Block diagram graphics displays are color-coded to identify signals that have been monitored or inferred to have nominal values, signals that are out of tolerance, and circuit cards and functions that are diagnosed as faulty. A hypertext-like scheme is used to allow the user to easily navigate through the space of diagrams and tables. Over 50 graphic and tabular displays have been implemented. REDEX is currently being evaluated in a stand-alone mode using simulated RE fault scenarios. It will soon be interfaced to the RE and tested in an online environment. When completed and fielded, REDEX will be a concrete example of the application of expert systems technology to the problem of improving performance and reducing the lifecycle costs of operating NASA's communications networks in the 1990s.

  14. REDEX - The ranging equipment diagnostic expert system

    NASA Astrophysics Data System (ADS)

    Luczak, Edward C.; Gopalakrishnan, K.; Zillig, David J.

    REDEX, an advanced prototype expert system that diagnoses hardware failures in the Ranging Equipment (RE) at NASA's Ground Network tracking stations is described. REDEX will help the RE technician identify faulty circuit cards or modules that must be replaced, and thereby reduce troubleshooting time. It features a highly graphical user interface that uses color block diagrams and layout diagrams to illustrate the location of a fault. A semantic network knowledge representation technique was used to model the design structure of the RE. A catalog of generic troubleshooting rules was compiled to represent heuristics that are applied in diagnosing electronic equipment. Specific troubleshooting rules were identified to represent additional diagnostic knowledge that is unique to the RE. Over 50 generic and 250 specific troubleshooting rules have been derived. REDEX is implemented in Prolog on an IBM PC AT-compatible workstation. Block diagram graphics displays are color-coded to identify signals that have been monitored or inferred to have nominal values, signals that are out of tolerance, and circuit cards and functions that are diagnosed as faulty. A hypertext-like scheme is used to allow the user to easily navigate through the space of diagrams and tables. Over 50 graphic and tabular displays have been implemented. REDEX is currently being evaluated in a stand-alone mode using simulated RE fault scenarios. It will soon be interfaced to the RE and tested in an online environment. When completed and fielded, REDEX will be a concrete example of the application of expert systems technology to the problem of improving performance and reducing the lifecycle costs of operating NASA's communications networks in the 1990s.

  15. REDEX: The ranging equipment diagnostic expert system

    NASA Astrophysics Data System (ADS)

    Luczak, Edward C.; Gopalakrishnan, K.; Zillig, David J.

    1989-04-01

    REDEX, an advanced prototype expert system that diagnoses hardware failures in the Ranging Equipment (RE) at NASA's Ground Network tracking stations is described. REDEX will help the RE technician identify faulty circuit cards or modules that must be replaced, and thereby reduce troubleshooting time. It features a highly graphical user interface that uses color block diagrams and layout diagrams to illustrate the location of a fault. A semantic network knowledge representation technique was used to model the design structure of the RE. A catalog of generic troubleshooting rules was compiled to represent heuristics that are applied in diagnosing electronic equipment. Specific troubleshooting rules were identified to represent additional diagnostic knowledge that is unique to the RE. Over 50 generic and 250 specific troubleshooting rules have been derived. REDEX is implemented in Prolog on an IBM PC AT-compatible workstation. Block diagram graphics displays are color-coded to identify signals that have been monitored or inferred to have nominal values, signals that are out of tolerance, and circuit cards and functions that are diagnosed as faulty. A hypertext-like scheme is used to allow the user to easily navigate through the space of diagrams and tables. Over 50 graphic and tabular displays have been implemented. REDEX is currently being evaluated in a stand-alone mode using simulated RE fault scenarios. It will soon be interfaced to the RE and tested in an online environment. When completed and fielded, REDEX will be a concrete example of the application of expert systems technology to the problem of improving performance and reducing the lifecycle costs of operating NASA's communications networks in the 1990's.

  16. REDEX: The ranging equipment diagnostic expert system

    NASA Technical Reports Server (NTRS)

    Luczak, Edward C.; Gopalakrishnan, K.; Zillig, David J.

    1989-01-01

    REDEX, an advanced prototype expert system that diagnoses hardware failures in the Ranging Equipment (RE) at NASA's Ground Network tracking stations is described. REDEX will help the RE technician identify faulty circuit cards or modules that must be replaced, and thereby reduce troubleshooting time. It features a highly graphical user interface that uses color block diagrams and layout diagrams to illustrate the location of a fault. A semantic network knowledge representation technique was used to model the design structure of the RE. A catalog of generic troubleshooting rules was compiled to represent heuristics that are applied in diagnosing electronic equipment. Specific troubleshooting rules were identified to represent additional diagnostic knowledge that is unique to the RE. Over 50 generic and 250 specific troubleshooting rules have been derived. REDEX is implemented in Prolog on an IBM PC AT-compatible workstation. Block diagram graphics displays are color-coded to identify signals that have been monitored or inferred to have nominal values, signals that are out of tolerance, and circuit cards and functions that are diagnosed as faulty. A hypertext-like scheme is used to allow the user to easily navigate through the space of diagrams and tables. Over 50 graphic and tabular displays have been implemented. REDEX is currently being evaluated in a stand-alone mode using simulated RE fault scenarios. It will soon be interfaced to the RE and tested in an online environment. When completed and fielded, REDEX will be a concrete example of the application of expert systems technology to the problem of improving performance and reducing the lifecycle costs of operating NASA's communications networks in the 1990's.

  17. Comparison of transferrin isoform analysis by capillary electrophoresis and HPLC for screening congenital disorders of glycosylation.

    PubMed

    Dave, Mihika B; Dherai, Alpa J; Udani, Vrajesh P; Hegde, Anaita U; Desai, Neelu A; Ashavaid, Tester F

    2018-01-01

    Transferrin, a major glycoprotein has different isoforms depending on the number of sialic acid residues present on its oligosaccharide chain. Genetic variants of transferrin as well as the primary (CDG) & secondary glycosylation defects lead to an altered transferrin pattern. Isoform analysis methods are based on charge/mass variations. We aimed to compare the performance of commercially available capillary electrophoresis CDT kit for diagnosing congenital disorders of glycosylation with our in-house optimized HPLC method for transferrin isoform analysis. The isoform pattern of 30 healthy controls & 50 CDG-suspected patients was determined by CE using a Carbohydrate-Deficient Transferrin kit. The results were compared with in-house HPLC-based assay for transferrin isoforms. Transferrin isoform pattern for healthy individuals showed a predominant tetrasialo transferrin fraction followed by pentasialo, trisialo, and disialotransferrin. Two of 50 CDG-suspected patients showed the presence of asialylated isoforms. The results were comparable with isoform pattern obtained by HPLC. The commercial controls showed a <20% CV for each isoform. Bland Altman plot showed the difference plot to be within +1.96 with no systemic bias in the test results by HPLC & CE. The CE method is rapid, reproducible and comparable with HPLC and can be used for screening Glycosylation defects. © 2017 Wiley Periodicals, Inc.

  18. 77 FR 67290 - Basic Service Tier Encryption Compatibility Between Cable Systems and Consumer Electronics Equipment

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-11-09

    ...-126] Basic Service Tier Encryption Compatibility Between Cable Systems and Consumer Electronics... between consumer electronics equipment (such as digital television sets) and newly encrypted cable service... Act''), Congress sought to make sure that consumer electronics equipment could receive cable...

  19. 33 CFR 164.43 - Automatic Identification System Shipborne Equipment-Prince William Sound.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 33 Navigation and Navigable Waters 2 2012-07-01 2012-07-01 false Automatic Identification System Shipborne Equipment-Prince William Sound. 164.43 Section 164.43 Navigation and Navigable Waters COAST GUARD... Automatic Identification System Shipborne Equipment—Prince William Sound. (a) Until December 31, 2004, each...

  20. 33 CFR 164.43 - Automatic Identification System Shipborne Equipment-Prince William Sound.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 33 Navigation and Navigable Waters 2 2013-07-01 2013-07-01 false Automatic Identification System Shipborne Equipment-Prince William Sound. 164.43 Section 164.43 Navigation and Navigable Waters COAST GUARD... Automatic Identification System Shipborne Equipment—Prince William Sound. (a) Until December 31, 2004, each...

  1. 33 CFR 164.43 - Automatic Identification System Shipborne Equipment-Prince William Sound.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 33 Navigation and Navigable Waters 2 2014-07-01 2014-07-01 false Automatic Identification System Shipborne Equipment-Prince William Sound. 164.43 Section 164.43 Navigation and Navigable Waters COAST GUARD... Automatic Identification System Shipborne Equipment—Prince William Sound. (a) Until December 31, 2004, each...

  2. Hot conditioning equipment conceptual design report

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Bradshaw, F.W., Westinghouse Hanford

    1996-08-06

    This report documents the conceptual design of the Hot Conditioning System Equipment. The Hot conditioning System will consist of two separate designs: the Hot Conditioning System Equipment; and the Hot Conditioning System Annex. The Hot Conditioning System Equipment Design includes the equipment such as ovens, vacuum pumps, inert gas delivery systems, etc.necessary to condition spent nuclear fuel currently in storage in the K Basins of the Hanford Site. The Hot Conditioning System Annex consists of the facility of house the Hot Conditioning System. The Hot Conditioning System will be housed in an annex to the Canister Storage Building. The Hotmore » Conditioning System will consist of pits in the floor which contain ovens in which the spent nuclear will be conditioned prior to interim storage.« less

  3. 76 FR 66666 - Basic Service Tier Encryption Compatibility Between Cable Systems and Consumer Electronics Equipment

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-10-27

    ... 11-153] Basic Service Tier Encryption Compatibility Between Cable Systems and Consumer Electronics... substantially affect compatibility between cable service and consumer electronics equipment for most subscribers... problems between cable service and consumer electronics equipment were limiting and/or precluding the...

  4. The integrated quality assessment of Chinese commercial dry red wine based on a method of online HPLC-DAD-CL combined with HPLC-ESI-MS.

    PubMed

    Yu, Hai-Xiang; Sun, Li-Qiong; Qi, Jin

    2014-07-01

    To apply an integrated quality assessment strategy to investigate the quality of multiple Chinese commercial dry red wine samples. A comprehensive method was developed by combining a high performance liquid chromatography-diode array detector-chemiluminescence (HPLC-DAD-CL) online hyphenated system with an HPLC-ESI-MS technique. Chromatographic and H2O2-scavenging active fingerprints of thirteen batches of different, commercially available Chinese dry red wine samples were obtained and analyzed. Twenty-five compounds, including eighteen antioxidants were identified and evaluated. The dominant and characteristic antioxidants in the samples were identified. The relationships between antioxidant potency and the cultivated variety of grape, producing area, cellaring period, and trade mark are also discussed. The results provide the feasibility for an integrated quality assessment strategy to be efficiently and objectively used in quality (especially antioxidant activity) assessment and identification of dry red wine. Copyright © 2014 China Pharmaceutical University. Published by Elsevier B.V. All rights reserved.

  5. Mobile Equipment Expands Inventory.

    ERIC Educational Resources Information Center

    McGough, Robert L.; And Others

    1978-01-01

    Describes the Mobile Equipment Modules (MEM) system in Duluth, Minnesota. MEM is a way to hold down costs and increase learning opportunities by consolidating purchases of expensive shop equipment within the school district, grouping the equipment in modules, and scheduling and moving it from school to school as needed. (MF)

  6. HPLC-DAD-MS identification of bioactive secondary metabolites from Ferula communis roots.

    PubMed

    Arnoldi, Lolita; Ballero, Mauro; Fuzzati, Nicola; Maxia, Andrea; Mercalli, Enrico; Pagni, Luca

    2004-06-01

    A simple HPLC method was developed to distinguish between 'poisonous' and 'non-poisonous' chemotypes of Ferula communis. The method was performed on a C8 reverse phase analytical column using a binary eluent (aqueous TFA 0.01%-TFA 0.01% in acetonitrile) under gradient condition. The two chemotypes showed different fingerprints. The identification of five coumarins and eleven daucane derivatives by HPLC-diode array detection (HPLC-DAD) and HPLC-MS is described. A coumarin, not yet described, was detected. Copyright 2004 Elsevier B.V.

  7. Identification of a Panax ginseng fruit fingerprint by HPLC-ESI-MS.

    PubMed

    Zhao, H F; Xu, F F; Guo, Y T; Mi, H

    2016-03-11

    Over many years, parts of Panax ginseng (root and rhizome) have been identified and applied for medical purposes as traditional Chinese herbal medicine. Recently, research has indicated that ginseng fruit also contains similar compounds and is as rich as the other parts of the ginseng. This discovery may dramatically improve the efficient of outputs derived from ginseng products. Here, a new technique combining high-performance liquid chromatography (HPLC) with electrospray ionization tandem mass spectrometry (ESI-MS) was employed to identify the fingerprint of P. ginseng fruit. Using HPLC, compounds that are important for medical purposes were extracted and purified. Combined with ESI-MS, the characteristic peaks (nine common peaks) of those compounds were identified, and the accuracy was confirmed by analysis using the Chromatographic Fingerprint Similarity Evaluation System (2004A edition). Overall, 15 batches of ginseng fruit had a similarity of more than 0.80, 13 batches of samples had a similarity between 0.97 and 0.99, and two batches had a similarity less than 0.90. The test solution and mobile phase selection was discussed. The HPLC-ESI-MS method can produce repeatable and reliable results and can be applied in the quality control of P. ginseng fruit.

  8. Satellite services system analysis study. Volume 3: Service equipment requirements

    NASA Technical Reports Server (NTRS)

    1981-01-01

    Service equipment mission requirements are discussed. On-orbit operations, satellite classes, and reference missions are included. Service equipment usage and requirements are considered. Equipment identification methodology is discussed. Service equipment usage is analyzed, including initial launch, revisit, Earth return, and orbital storage. A summary of service requirements and equipment is presented, including service equipment status, even interaction, satellite features, and observations.

  9. Equipped for the Future: A Collaborative Journey toward System Reform.

    ERIC Educational Resources Information Center

    NIFL News, 1997

    1997-01-01

    This issue of the National Institute for Literacy (NIFL) News is devoted to "Equipped for the Future" (EFF), the organization's long-term collaborative initiative for system reform. The newsletter contains the testimony of people who have been involved in the process over the past 2 years, speaking of the promise they see in EFF. The…

  10. DETERMINATION OF VENLAFAXINE, VILAZODONE AND THEIR MAIN ACTIVE METABOLITES IN HUMAN SERUM BY HPLC-DAD AND HPLC-MS.

    PubMed

    Petruczynik, Anna; Wroblewski, Karol; Szultka-Mlynska, Malgorzata; Buszewsk, Boguslaw; Karakula-Juchnowicz, Hanna; Gajewski, Jacek; Morylowska-Topolska, Justyna; Waksmundzka-Hajnosi, Monika

    2017-05-01

    A high performance liquid chromatography (HPLC) method for simultaneous analysis of venlafaxine and its major metabolite 0-desmethylvenlafaxine and vilazodone and its methabolite M10 have been devel- oped and validated. Chromatography was performed on the Phenyl-Hexyl column with mobile phase containing methanol, acetate buffer at pH 3.5 and diethylamine. The application of stationary phase with 7r-7c moieties and mobile phase containing diethylamine as silanol blocker lets to obtain double protection against silanols and thus very high theoretical plate numbers were obtained. The good separation selectivity, good peaks' symmetry and very high systems efficiency for all investigated compounds were obtained in applied chromatographic system. The method is very efficient and suitable for the analysis of investigated drugs and their metabolites in human serum for patients' pharmacotherapy control.

  11. Transfusion associated peak in hb HPLC chromatogram - a case report.

    PubMed

    Jain, Sonal; Dass, Jasmita; Pati, Hara Prasad

    2012-01-01

    High performance liquid chromatography (HPLC) and electrophoresis are commonly used to diagnose various hemoglobinopathies. However, insufficient information about the transfusion history can lead to unexpected and confusing results. We are reporting a case of Juvenile myelomonocytic leukemia (JMML) in which HbHPLC was done to quantify fetal hemoglobin (HbF). The chromatogram showed elevated HbF along with a peak in the HbD window. A transfusion acquired peak was suspected based on the unexpectedly low percentage of HbD and was subsequently confirmed using parental HbHPLC.

  12. Using HPLC-Mass Spectrometry to Teach Proteomics Concepts with Problem-Based Techniques

    ERIC Educational Resources Information Center

    Short, Michael; Short, Anne; Vankempen, Rachel; Seymour, Michael; Burnatowska-Hledin, Maria

    2010-01-01

    Practical instruction of proteomics concepts was provided using high-performance liquid chromatography coupled with a mass selective detection system (HPLC-MS) for the analysis of simulated protein digests. The samples were prepared from selected dipeptides in order to facilitate the mass spectral identification. As part of the prelaboratory…

  13. A study on the real-time reliability of on-board equipment of train control system

    NASA Astrophysics Data System (ADS)

    Zhang, Yong; Li, Shiwei

    2018-05-01

    Real-time reliability evaluation is conducive to establishing a condition based maintenance system for the purpose of guaranteeing continuous train operation. According to the inherent characteristics of the on-board equipment, the connotation of reliability evaluation of on-board equipment is defined and the evaluation index of real-time reliability is provided in this paper. From the perspective of methodology and practical application, the real-time reliability of the on-board equipment is discussed in detail, and the method of evaluating the realtime reliability of on-board equipment at component level based on Hidden Markov Model (HMM) is proposed. In this method the performance degradation data is used directly to realize the accurate perception of the hidden state transition process of on-board equipment, which can achieve a better description of the real-time reliability of the equipment.

  14. [Study on the fingerprint of Morus alba from different habitats by HPLC].

    PubMed

    Chen, Cheng; Li, Hong-Bo; Wang, Liu-Ping; Li, Yun-Rong; Xin, Ning

    2012-12-01

    To establish HPLC fingerprint of Morus alba from different habitats by HPLC and provide basis for its quality control. HPLC analysis was performed on an Agilent XDB C18 Column (4.6 mm x 250 mm, 5 microm), gradient eluted composed of acetonitrile and 0.3% phosphate acid. The column temperature was set at 35 degrees C and the flow rate was 0.5 mL/min. The detective wavelength was 290 nm. The HPLC fingerprint for 10 batches of Morus alba was studied on their similarity. There were twelve common peaks in the fingerprint. The similarity of 7 batches was above 0.9 and the other batches had low similarity. The HPLC fingerprint can be used for quality control of Morus alba with high characteristics and specificity.

  15. Quantification of the Triazole Antifungal Compounds Voriconazole and Posaconazole in Human Serum or Plasma Using Liquid Chromatography Electrospray Tandem Mass Spectrometry (HPLC-ESI-MS/MS).

    PubMed

    Molinelli, Alejandro R; Rose, Charles H

    2016-01-01

    Voriconazole and posaconazole are triazole antifungal compounds used in the treatment of fungal infections. Therapeutic drug monitoring of both compounds is recommended in order to guide drug dosing to achieve optimal blood concentrations. In this chapter we describe an HPLC-ESI-MS/MS method for the quantification of both compounds in human plasma or serum following a simple specimen preparation procedure. Specimen preparation consists of protein precipitation using methanol and acetonitrile followed by a cleanup step that involves filtration through a cellulose acetate membrane. The specimen is then injected into an HPLC-ESI-MS/MS equipped with a C18 column and separated over an acetonitrile gradient. Quantification of the drugs in the specimen is achieved by comparing the response of the unknown specimen to that of the calibrators in the standard curve using multiple reaction monitoring.

  16. PREPARATIVE ISOLATION AND PURIFICATION OF CHEMICAL CONSTITUENTS OF BELAMCANDA BY MPLC, HSCCC AND PREP-HPLC

    PubMed Central

    Wang, Xiaohong; Liang, Yong; Peng, Cuilin; Xie, Huichun; Pan, Man; Zhang, Tianyou; Ito, Yoichiro

    2010-01-01

    Combined with medium-pressure liquid chromatography (MPLC) and preparative high-pressure liquid chromatography (Prep-HPLC), high-speed countercurrent chromatography (HSCCC) was successfully applied for separation and purification of isoflavonoids from the extract of belamcanda. HSCCC separation was performed on a two-phase solvent system composed of methyl tert-butyl ether -ethyl acetate - n-butyl alcohol – acetonitrile −0.1% aqueous trifluoroacetic acid at a volume radio of 1:2:1:1:5. Semi-purified peak fractions from HSCCC separation were further purified by Prep-HPLC. Nine well-separated fractions were analyzed by HPLC-UV absorption spectrometry to determine their purities and characterized with ESI-MSn. Except for peaksland VII (unknown) seven compounds were identified as apocynin (peak II), mangiferin (peak III), 7-O-methylmangiferin (peak IV), hispidulin (peak V), 3′-hydroxyltectoridin (peak VI), iristectorin B (peak VII), isoiridin (peak IX). PMID:21552369

  17. Analysis of the constituents of aqueous preparations of Stachys recta by HPLC-DAD and HPLC-ESI-MS.

    PubMed

    Karioti, Anastasia; Bolognesi, Letizia; Vincieri, Franco Francesco; Bilia, Anna Rita

    2010-09-21

    In the present study a method based on liquid chromatography with diode array detection (HPLC/DAD) coupled to an electrospray ionization (ESI) interface for the simultaneous determination of phenolic constituents in three aqueous preparations of the herbal medicinal drug Stachys recta. The developed assay was simple and effective and permitted the quality control of S. recta decoctions and infusion. Overall, 30 constituents were detected and identified, belonging mainly to three classes of compounds: caffeoylquinic acids, phenylethanol glycosides and flavonoids. 15 of them were quantified having a lower limit not less than 0.02% of the lyophilized extracts. Only seven of them were previously reported in this species, while 23 were identified for the first time as constituents of S. recta. HPLC-DAD-ESI-MS analysis provided evidence for the certain identification of the main constituents and in some cases of their isomers. Eight constituents were isolated and their structure elucidated by HPLC-ESI-MS and 1D- and 2D-NMR spectroscopy. Among the investigated preparations, the infusion seems to be the best method to extract the native constituents of the plant, while decoction is a more aggressive treatment and causes partial degradation of some acylated flavonoids. Copyright 2010 Elsevier B.V. All rights reserved.

  18. HPLC-DAD and HPLC-ESI-MS/MS methods for metabolite profiling of propolis extracts.

    PubMed

    Pellati, Federica; Orlandini, Giulia; Pinetti, Diego; Benvenuti, Stefania

    2011-07-15

    In this study, the composition of polyphenols (phenolic acids and flavonoids) in propolis extracts was investigated by HPLC-DAD and HPLC-ESI-MS/MS by comparing the performance of ion trap and triple quadrupole mass analyzers. The analyses were carried out on an Ascentis C(18) column (250mm×4.6mm I.D., 5μm), with a mobile phase composed by 0.1% formic acid in water and acetonitrile. Overall, the UV spectra, the MS and MS/MS data allowed the identification of 40 compounds. In the case of flavonoids, the triple quadrupole mass analyzer provided more collision energy if compared with the ion trap, originating product ions at best sensitivity. The HPLC method was validated in agreement with ICH guidelines: the correlation coefficients were >0.998; the limit of detection was in the range 1.6-4.6μg/ml; the recovery range was 96-105%; the intra- and inter-day %RSD values for retention times and peak areas were found to be <0.3 and 1.9%, respectively. The developed technique was applied to the analysis of hydroalcoholic extracts of propolis available on the Italian market. Although the chromatographic profile of the analyzed samples was similar, the quantitative analysis indicated that there is a great variability in the amount of the active compounds: the content of total phenolic acids ranged from 0.17 to 16.67mg/ml and the level of total flavonoids from 2.48 to 41.10mg/ml. The proposed method can be considered suitable for the phytochemical analysis of propolis extracts used in phytotherapy. Copyright © 2011 Elsevier B.V. All rights reserved.

  19. 78 FR 41434 - Proposed Revisions to Design of Structures, Components, Equipment and Systems

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-07-10

    ..., Components, Equipment and Systems AGENCY: Nuclear Regulatory Commission. ACTION: Standard review plan-draft... Systems, Piping Components and their Associated Supports,'' of NUREG-0800, ``Standard Review Plan for the Review of Safety Analysis Reports for Nuclear Power Plants: LWR Edition.'' DATES: Submit comments by...

  20. Comparison of UHPLC and HPLC in benzodiazepines analysis of postmortem samples: a case-control study.

    PubMed

    Behnoush, Behnam; Sheikhazadi, Ardeshir; Bazmi, Elham; Fattahi, Akbar; Sheikhazadi, Elham; Saberi Anary, Seyed Hossein

    2015-04-01

    The aim of this study was to compare system efficiency and analysis duration regarding the solvent consumption and system maintenance in high-pressure liquid chromatography (HPLC) and ultra high-pressure liquid chromatography (UHPLC). In a case-control study, standard solutions of 7 benzodiazepines (BZs) and 73 biological samples such as urine, tissue, stomach content, and bile that screened positive for BZs were analyzed by HPLC and UHPLC in laboratory of forensic toxicology during 2012 to 2013. HPLC analysis was performed using a Knauer by 100-5 C-18 column (250 mm × 4.6 mm) and Knauer photodiode array detector (PAD). UHPLC analysis was performed using Knauer PAD detector with cooling autosampler and Eurospher II 100-3 C-18 column (100 mm × 3 mm) and also 2 pumps. The mean retention time, standard deviation, flow rate, and repeatability of analytical results were compared by using 2 methods. Routine runtimes in HPLC and UHPLC took 40 and 15 minutes, respectively. Changes in mobile phase composition of the 2 methods were not required. Flow rate and solvent consumption in UHPLC decreased. Diazepam and flurazepam were detected more frequently in biological samples. In UHPLC, small particle size and short length of column cause effective separation of BZs in a very short time. Reduced flow rate, solvent consumption, and injection volume cause more efficiency and less analysis costs. Thus, in the detection of BZs, UHPLC is an accurate, sensitive, and fast method with less cost of analysis.

  1. A novel HPLC fluorescence method for the quantification of methylphenidate in human plasma

    PubMed Central

    Zhu, Hao-Jie; Wang, Jun-Sheng; Patrick, Kennerly S.; Donovan, Jennifer L.; DeVane, C. Lindsay; Markowitz, John S.

    2007-01-01

    A number of analytical methods have been established to quantify methylphenidate (MPH). However, to date no HPLC methods are applicable to human pharmacokinetic studies without the use of mass spectrometry (MS) detection. We developed a sensitive and reliable HPLC-fluorescence method for the determination of MPH in human plasma using 4-(4,5-diphenyl-1H-imidazol-2-yl) benzoyl chloride (DIB-Cl) as the derivatizing agent. An established GC-MS method was adopted in this study as a comparator assay. MPH was derivatized DIB-Cl, and separated isocratically on a C18 column using a HPLC system with fluorescence detection (λex: 330 nm, λem: 460 nm). The lower limit of determination was found to be 1 ng/mL. A linear calibration curve was obtained over the concentrations ranging from 1 to 80 ng/mL (r=0.998). The relative standard deviations of intra-day and inter-day variations were ≤ 9.10 % and ≤ 7.58 %, respectively. The accuracy ranged between 92.59 % and 103.06 %. The method was successfully applied to the pharmacokinetic study of a subject who received a single oral dose (0.3 mg/kg) of immediate-release MPH and yielded consistent results with that of the GC-MS method. This method is the first HPLC assay with non-MS detection providing sufficient reliability and sensitivity for both pre-clinical and clinical studies of MPH. PMID:17804308

  2. Comparison of UPLC and HPLC methods for determination of vitamin C.

    PubMed

    Klimczak, Inga; Gliszczyńska-Świgło, Anna

    2015-05-15

    Ultra performance liquid chromatography (UPLC) and high-performance liquid chromatography (HPLC) methods for determination of ascorbic acid (AA) and total AA (TAA) contents (as the sum of AA and dehydroascorbic acid (DHAA) after its reduction to AA) in fruit beverages and in pharmaceutical preparations were compared. Both methods are rapid: total time of analysis was 15 and 6 min for HPLC and UPLC methods, respectively. The methods were validated in terms of linearity, instrument precision, limits of detection (LOD) and quantification (LOQ), accuracy and recovery. Intra- and inter-day instrument precisions for fruit juices, expressed as RSD, were 2.2% and 2.4% for HPLC, respectively, and 1.7% and 1.9% for UPLC, respectively. For vitamin C tablets, inter- and intra-day precisions were 0.4% and 0.5%, respectively (HPLC), and 0.5% and 0.3%, respectively (UPLC). Both methods were sensitive: LOD was 0.049 μg/mL for HPLC and 0.024 μg/mL for UPLC while LOQs were 0.149 and 0.073 μg/mL for HPLC and UPLC, respectively. These methods could be useful in the routine qualitative and quantitative analysis of AA or TAA in pharmaceutical preparations or fruit beverages. However, UPLC method is more sensitive, faster and consumes less eluent. Copyright © 2014 Elsevier Ltd. All rights reserved.

  3. HPLC determination of caffeine in coffee beverage

    NASA Astrophysics Data System (ADS)

    Fajara, B. E. P.; Susanti, H.

    2017-11-01

    Coffee is the second largest beverage which is consumed by people in the world, besides the water. One of the compounds which contained in coffee is caffeine. Caffeine has the pharmacological effect such as stimulating the central nervous system. The purpose of this study is to determine the level of caffeine in coffee beverages with HPLC method. Three branded coffee beverages which include in 3 of Top Brand Index 2016 Phase 2 were used as samples. Qualitative analysis was performed by Parry method, Dragendorff reagent, and comparing the retention time between sample and caffeine standard. Quantitative analysis was done by HPLC method with methanol-water (95:5v/v) as mobile phase and ODS as stationary phasewith flow rate 1 mL/min and UV 272 nm as the detector. The level of caffeine data was statistically analyzed using Anova at 95% confidence level. The Qualitative analysis showed that the three samples contained caffeine. The average of caffeine level in coffee bottles of X, Y, and Z were 138.048 mg/bottle, 109.699 mg/bottle, and 147.669 mg/bottle, respectively. The caffeine content of the three coffee beverage samples are statistically different (p<0.05). The levels of caffeine contained in X, Y, and Z coffee beverage samples were not meet the requirements set by the Indonesian Standard Agency of 50 mg/serving.

  4. Study and development of reversed-phase HPLC systems for the determination of 2-imidazolines in the presence of preservatives in pharmaceutical preparations.

    PubMed

    Antoniou, Constantinos G; Markopoulou, Catherine K; Kouskoura, Maria G; Koundourellis, John E

    2011-01-01

    Different HPLC chromatographic systems were investigated on a C18 ACE 5 pm, 150 x 4.6 mm id column for the determination of tymazoline, tramazoline, and antazoline, with either naphazoline or xylometazoline, in commercial preparations. For the development and optimization of the systems, a Response Surface Method (r=0.925-0.980) was used to illustrate the changes in k as a function of pH values and different salt concentrations. The simultaneous separation of 2-imidazolines was accomplished at 40 degrees C with 0.01 M ammonium acetate-methanol (50+50, v/v, pH 6.0) mobile phase at a flow rate of 1.2 mL/min. In order to deal with the usual coexistence of 2-imidazolines with benzethonium and benzalkonium chloride preservatives, it was necessary to use another chromatographic system, 0.01 M ammonium acetate-methanol (50+50, v/v) mobile phase on a cyano ACE 5 pm, 150 x 4.6 mm id column. As part of a more thorough theoretical investigation, a partial least-squares (PLS) technique was used for modeling the RP-HPLC retention data. The model was based on molecular structure descriptors of the analytes' X variables and on their retention time (Log K) Y. The goodness of fit was estimated by the PLS correlation coefficient (r2) and root mean square error of estimation values, which were 0.994 and 0.0479, respectively.

  5. Development of a Communication System Compatible with Chemical Protective Clothing and Equipment.

    DTIC Science & Technology

    1986-06-23

    for the Chemical Protective Clothing Communication System are discussed in the operation manuals for the engineering prototypes of the trans- ceiver...Report Date DEVELOPMENT OF A COMMUNICATION SYSTEM COMPATIBLE June 1986 WITH CHEMICAL PROTECTIVE CLOTHING AND EQUIPMENT 6. Performing Organization Code 8...Abstract - ’The U. S. Coast Guard and NASA joined in a project to develop a cominications system to operate inside protective suits used in chemical spill

  6. [HPLC fingerprint analysis of flavonoids of phyllanthi fructus from different habitats].

    PubMed

    Wang, Fei; Wang, Shuai; Meng, Xian-sheng; Bao, Yong-rui; Zhu, Ying-huan

    2014-11-01

    To establish the HPLC fingerprint of flavonoids of Phyllanthi Fructus from different habitats. HPLC method was adopted. The flavonoids composition of Phyllanthi Fructus from 10 different habitats was determined on an Agilent C, chromatographic column with 0. 5% formic acid water (A)-acetonitrile (B) as the mobile phase in gradient elution under the wavelength of 254 nm. The HPLC fingerprints of flavonoids composition of Phyllanthi Fructus were established to evaluate the qualitiy of them. The HPLC fingerprints of flavonoids composition of Phyllanthi Fructus from 10 different habitats were established. 18 common peaks were found and the similarities of them were more than 0. 90 except the ones from Guangxi and Guangdong. The method is simple, accurate and repeatable. It can be used for research and quality control of the effective components in Phyllanthi Fructus.

  7. Analysis of munitions constituents in IMX formulations by HPLC and HPLC-MS.

    PubMed

    Russell, A L; Seiter, J M; Coleman, J G; Winstead, B; Bednar, A J

    2014-10-01

    The use of Insensitive Munitions eXplosives (IMX) is increasing as the Army seeks to replace certain conventional munitions constituents, such as 2,4,6-trinitrotolene (TNT), for improved safety. The IMX formulations are more stable and therefore less prone to accidental detonation while designed to match the performance of legacy materials. Two formulations, IMX 101 and 104 are being investigated as a replacement for TNT in artillery rounds and composition B Army mortars, respectively. The chemical formulations of IMX-101 and 104 are comprised of four constituents;2,4-dinitroanisole (DNAN), 3-nitro-1,2,4-triazol-5-one (NTO), 1-nitroguanidine (NQ), and Hexahydro-1,3,5-trinitro-1,3,5-triazine (RDX) which are mixed in various ratios to achieve the desired performance. The current work details the analysis of the IMX constituents by single column HPLC-UV-ESI-MS. Detection limits determined are in agreement with similar HPLC analysis of compounds, ranging from 7 to 9μg/L. Gradient mobile phases are used to allow separation of the 4 target compounds in more complex mixture of other concomitant compounds. Mass spectra are used to confirm analyte identity with chromatographic retention time. Published by Elsevier B.V.

  8. Office of Space Flight standard spaceborne Global Positioning System user equipment project

    NASA Technical Reports Server (NTRS)

    Saunders, Penny E.

    1991-01-01

    The Global Positioning System (GPS) provides users autonomous, real-time navigation capability. A vehicle equipped with GPS user equipment can receive and process signals transmitted by a constellation of GPS satellites and derive from the resulting measurements the vehicle's position and velocity. Specified accuracies range from 16 to 76 meters and 0.1 to 1.0 meters/second for position and velocity, respectively. In a rendezvous and docking scenario, the use of a technique called relative GPS can provide range and range rate accuracies on the order of 1 meter and 0.01 meters/second, respectively. Relative GPS requires both vehicles to be equipped with GPS user equipment and a data communication link for transmission of GPS data and GPS satellite selection coordination information. Through coordinated satellite selection, GPS measurement errors common to both users are cancelled and improved relative position and velocity accuracies are achieved. The background, the design approach, the expected performance and capabilities, the development plan, and the project status are described. In addition, a description of relative GPS, the possible GPS hardware and software configurations, and its application to automated rendezvous and capture are presented.

  9. [Study on HPLC fingerprint of Oldenlandia diffusa].

    PubMed

    Chen, Yan; Yao, Zhi-Hong; Dai, Yi; Cheng, Hong; Wen, Li-Rong; Zhou, Guang-Xiong; Yao, Xin-Sheng

    2012-06-01

    To establish the HPLC fingerprint chromatogram of Oldenlandia diffusa coupled with chemometrics means for the quality control of multi-batches of medicinal material. The separation was developed on C18 column(4.6 mm x 250 mm, 5 microm) by gradient elution with acetonitrile-water(both containing 0.1 per thousand (V/V) ocetic acid) as mobile phase at a flow rate of 0.8 mL/min, the detection wavelength at 238 nm and column temperature at 30 degrees C. The HPLC fingerprint chromatogram of Oldenlandia diffusa was set up and the main characteristic peaks were identified by comparing with chemical reference substance. The quality of 22 batches of medicinal material was evaluated by similarity assay as well as principal component analysis (PCA) and cluster analysis. The established HPLC fingerprint chromatogram of Oldenlandia diffusa was specific, precise, reproducible and stable. 11 peaks were chemically identified. The similarity of 17 batches of Oldenlandia diffusa was obviously higher than 5 batches of adulterants. PCA showed that 17 batches of Oldenlandia diffusa were in a domain and 5 batches of adulterants were far apart from the domain. The cluster analysis of the 22 batches of medicinal material showed that 17 batches of Oldenlandia diffusa were in a cluster while 5 batches of adulterants were excluded. Further cluster analysis was carried out for the quality consistency of 17 batches of Oldenlandia diffusa and accordingly they were devided into 4 clusters. With the combination of chemometrics means, the HPLC fingerprint chromatogram provides a method for evaluation of authenticity and quality control of Oldenlandia diffusa, which is favorable to improve overall quality control of Oldenlandia diffusa.

  10. Online identification of chlorogenic acids, sesquiterpene lactones, and flavonoids in the Brazilian arnica Lychnophora ericoides Mart. (Asteraceae) leaves by HPLC-DAD-MS and HPLC-DAD-MS/MS and a validated HPLC-DAD method for their simultaneous analysis.

    PubMed

    Gobbo-Neto, Leonardo; Lopes, Norberto P

    2008-02-27

    Lychnophora ericoides Mart. (Asteraceae, Vernonieae) is a plant, endemic to Brazil, with occurrence restricted to the "cerrado" biome. Traditional medicine employs alcoholic and aqueous-alcoholic preparations of leaves from this species for the treatment of wounds, inflammation, and pain. Furthermore, leaves of L. ericoides are also widely used as flavorings for the Brazilian traditional spirit "cachaça". A method has been developed for the extraction and HPLC-DAD analysis of the secondary metabolites of L. ericoides leaves. This analytical method was validated with 11 secondary metabolites chosen to represent the different classes and polarities of secondary metabolites occurring in L. ericoides leaves, and good responses were obtained for each validation parameter analyzed. The same HPLC analytical method was also employed for online secondary metabolite identification by HPLC-DAD-MS and HPLC-DAD-MS/MS, leading to the identification of di- C-glucosylflavones, coumaroylglucosylflavonols, flavone, flavanones, flavonols, chalcones, goyazensolide, and eremantholide-type sesquiterpene lactones and positional isomeric series of chlorogenic acids possessing caffeic and/or ferulic moieties. Among the 52 chromatographic peaks observed, 36 were fully identified and 8 were attributed to compounds belonging to series of caffeoylferuloylquinic and diferuloylquinic acids that could not be individualized from each other.

  11. [HPLC Fingerprint of QingGuangAn and Determination of the Main Components].

    PubMed

    Wang, Min; Shen, Bing-bing; Luo, Juan; Chen, Yang; Yang, Yu-pei; Chen, Sheng-huang

    2015-10-01

    To establish an HPLC fingerprint of ethanol extract of QingGuangAn, and to determine the contents of paeoniflorin and calycosin-7-glucosid. HPLC analysis was performed on an Agilent 1260 Infinity LC system and carried out at 35 degrees C on a column of GRACE Alltima C18 (250 mm x 4.6 mm, 5 μm). A binary gradient elution system was composed of acetonitrile (phase A) and water solution (phase B). Detection was performed at the wavelength of 254 nm, the mobile flow rate was 0.8 mL/min. A matrix including 20 variations (characteristic peaks area) and 10 samples was constructed for similarity evaluation. The results showed that the collected samples had a good similarity. A specificity fingerprint was produced and 20 characteristic peaks were designated. The content of paeoniflorin and calycosin-7-glucosid was 0.368 and 0.049 mg/g, respectively. It is a reliable, available and quick method for quality control of QingGuangAn,which provides some reference for the comparison of different extracting methods of QingGuangAn and the differences of pharmacodynamic.

  12. [Determination of rhynchophylline and isorhynchophylline in Uncaria rhynchophylla by HPLC].

    PubMed

    Yang, Xiu-Juan; Hong, Yan-Long; Wu, Fei; Ruan, Ke-Feng; Feng, Yi

    2013-03-01

    To explore an HPLC method for determination of rhnchophylline and isorhnchophylline in Uncaria rhnchophylla. An HPLC method has been developed for determination of rhnchophylline and isorhnchophylline. The transformation of rhnchophylline and isorhnchophylline after heating was also studied by HPLC-ESI-MS. Good linearities of rhynchophylline and isorhynchophylline were 0.064-5.100, 0.064-5.110 mg, respectively. The average recoveries were from 87.51% to 88.83% for rhynchophylline and from 107.9% to 113.9% for isorhynchophylline. The recoveries of rhynchophylline and isorhnchophylline reference solutions after extraction were 12.60% and 40.00% in the reflux extraction procedure, respectively. While in the ultrasonic extraction procedure, the average recoveries of rhynchophylline and isorhynchophylline was from 99.48% to 103.2% and from 97.00% to 99.59%, resepectively. The recoveries of rhynchophylline and isorhnchophylline reference solutions after extraction were 47.08% and 51.03%, respectively. The unqualified recovery could be elucidated by HPLC-ESI-MS analysis, indicating that trhynchophylline could be transformed mostly into isorhynchophylline and a little amount of unkown composition, while isorhynchophylline could be transformed into rhynchophylline isocorynoxeine, corynoxeine and 22-O-beta-D-glucopyranosyl isocorynoxeinic acid during the extraction procedure. Ultrasonic extraction procedure was more sutble for HPLC determination of the content of rhynchophylline and isorhynchophylline in U. rhnchophylla, however, the recovery problems should be paid attention to when it comes to the determination.

  13. Determination of anthocyanins from camu-camu (Myrciaria dubia) by HPLC-PDA, HPLC-MS, and NMR.

    PubMed

    Zanatta, Cinthia Fernanda; Cuevas, Elyana; Bobbio, Florinda O; Winterhalter, Peter; Mercadante, Adriana Z

    2005-11-30

    Camu-camu [Myrciaria dubia (HBK) McVaugh] is a small fruit native to the Amazonian rain forest. Its anthocyanin profile has now been investigated for the first time. Fruits from two different regions of the São Paulo state, Brazil, were analyzed. The major anthocyanins were isolated by high-speed countercurrent chromatography. HPLC-PDA, HPLC-MS/MS, and 1H NMR were used to confirm the identity of the main anthocyanins of camu-camu. Cyanidin-3-glucoside was identified as the major pigment in the fruits from both regions, representing 89.5% in the fruits produced in Iguape and 88.0% in those from Mirandópolis, followed by the delphinidin-3-glucoside, ranging between 4.2 and 5.1%, respectively. Higher total anthocyanin contents were detected in the fruits from Iguape (54.0 +/- 25.9 mg/100 g) compared to those from Mirandópolis (30.3 +/- 6.8 mg/100 g), most likely because of the lower temperatures in the Iguape region.

  14. Comparative tests of bench equipment for fuel control system testing of gas-turbine engine

    NASA Astrophysics Data System (ADS)

    Shendaleva, E. V.

    2018-04-01

    The relevance of interlaboratory comparative researches is confirmed by attention of world metrological community to this field of activity. Use of the interlaboratory comparative research methodology not only for single gages collation, but also for bench equipment complexes, such as modeling stands for fuel control system testing of gas-turbine engine, is offered. In this case a comparative measure of different bench equipment will be the control fuel pump. Ensuring traceability of measuring result received at test benches of various air enterprises, development and introduction of national standards to practice of bench tests and, eventually, improvement of quality and safety of a aircraft equipment is result of this approach.

  15. HPLC-ELSD Quantification and Centrifugal Partition Chromatography Isolation of 8-O-Acetylharpagide from Oxera coronata (Lamiaceae).

    PubMed

    Remeur, Camille; Le Borgne, Erell; Gauthier, Léa; Grougnet, Raphaël; Deguin, Brigitte; Poullain, Cyril; Litaudon, Marc

    2017-05-01

    Iridoid glycosides possess highly functionalised monoterpenoid aglycon with several contiguous stereocentres. For the most common, they are often present in quantities reaching several percentage of the fresh plant weight, and thus they may be regarded as starting material for the synthesis of a number of new chiral and bioactive molecules. To quantify and to isolate 8-O-acetylharpagide (AH) from several extracts of Oxera coronata R.P.J. de Kok, a Lamiaceae species endemic to New Caledonia, using HPLC-ELSD (evaporative light scattering detector) and centrifugal partition chromatography (CPC). Oxera coronata produces high amounts of AH in leaves, twigs and fruits. Water and methanol extracts of these plant parts were prepared. The content of AH in each extract was quantified by HPLC-ELSD, using acetonitrile-water (+0.1% formic acid) gradient elution. The HPLC method was validated for precision, linearity, limit of detection (LOD), limit of quantification (LOQ) and accuracy. A ternary solvent system ethyl acetate/n-propanol/water (3:2:5, v/v/v) was selected and applied to recover the target compound using Spot CPC from the leaves aqueous extract. HPLC-ELSD analysis followed by CPC purification led to the efficient isolation of AH from O. coronata leaves aqueous extract. HPLC-ELSD has proven to be a well-adapted detection and quantification method for iridoid glycosides, while CPC confirmed to be an efficient technique for the isolation of polar compounds. Copyright © 2016 John Wiley & Sons, Ltd. Copyright © 2016 John Wiley & Sons, Ltd.

  16. 42 CFR 493.1252 - Standard: Test systems, equipment, instruments, reagents, materials, and supplies.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 42 Public Health 5 2014-10-01 2014-10-01 false Standard: Test systems, equipment, instruments, reagents, materials, and supplies. 493.1252 Section 493.1252 Public Health CENTERS FOR MEDICARE & MEDICAID SERVICES, DEPARTMENT OF HEALTH AND HUMAN SERVICES (CONTINUED) STANDARDS AND CERTIFICATION LABORATORY REQUIREMENTS Quality System for Nonwaived...

  17. Selenium speciation analysis of Misgurnus anguillicaudatus selenoprotein by HPLC-ICP-MS and HPLC-ESI-MS/MS

    USDA-ARS?s Scientific Manuscript database

    Analytical methods for selenium (Se) speciation were developed using high performance liquid chromatography (HPLC) coupled to either inductively coupled plasma mass spectrometry (ICP-MS) or electrospray ionization tandem mass spectrometry (ESI-MS/MS). Separations of selenomethionine (Se-Met) and sel...

  18. [Study of the content of flavonoids of different parts in Saussures involucrata and their HPLC fingerprint chromatogram].

    PubMed

    Huang, Yi; Zhou, Qian; Yan, Ming; Xu, Fang; Kang, Airong; Yan, Huan; Hong, Lijun; Wang, Xintang; Zhong, Jie

    2005-11-01

    To determine the content of flavonoids and rutin in the different parts of Saussurea involucrata, and to establish their HPLC fingerprint chromatogram for the further development and utilization of the leaf. The content of flavonoids was determined with UV. The similarity evaluation system for chromatographic fingerprint of TCM was used to calculate similar degree of the HPLC chromatogram of different parts. The content of flavonoids and rutin is relatively high in the leaf. The similarity between leaf and the whole grass is 0. 812.

  19. Sustained Release Oral Nanoformulated Green Tea for Prostate Cancer Prevention

    DTIC Science & Technology

    2012-05-01

    31.25 ng/ml. The calibration samples were measured twice. The HPLC was a Shimadzu Prominence LC system containing a CBM-20A system controller...MDS SCIEX Ontario, Canada) equipped with a Turbo V Source and Turbo Ion Spray was coupled to the HPLC . The mass spectrometer was operated in...treated mice were subjected to HPLC and GC mass spectrometry for analysis of pharmacokinetic distribution of EGCG and bioavailability. Though we have

  20. Transfusion Associated Peak in Hb HPLC Chromatogram – a Case Report

    PubMed Central

    Jain, Sonal; Dass, Jasmita; Pati, Hara Prasad

    2012-01-01

    High performance liquid chromatography (HPLC) and electrophoresis are commonly used to diagnose various hemoglobinopathies. However, insufficient information about the transfusion history can lead to unexpected and confusing results. We are reporting a case of Juvenile myelomonocytic leukemia (JMML) in which HbHPLC was done to quantify fetal hemoglobin (HbF). The chromatogram showed elevated HbF along with a peak in the HbD window. A transfusion acquired peak was suspected based on the unexpectedly low percentage of HbD and was subsequently confirmed using parental HbHPLC. PMID:22348188

  1. Phototoxicity testing by online irradiation and HPLC.

    PubMed

    Schröder, Sven; Surmann, J P

    2006-11-01

    A high-performance liquid chromatography (HPLC) system was developed for the determination of drug photostability and phototoxicity based on an automated column-switching system with aqueous online UV-A irradiation and hyphenated organic separation of the drug and its photoproducts. The photoreactor is built with an poly(ethylene-co-tetrafluoroethylene) (ETFE) reaction coil knitted around a UV-A light source. The chromatographic separation was performed with two special C18 columns, which are also suitable for using with pure water as eluent. Degradation of chlorpromazine (CPZ) by ultraviolet light was investigated at pH 7 and pH 3. Furthermore chlorpromazine was irradiated in the presence of guanosine-5-monophosphate (GMP) in pH 7 buffered solution, leading to a new photoproduct. In the pH 3 irradiation studies of CPZ and GMP, no reaction was detected between the molecules.

  2. A Study of Parallels Between Antarctica South Pole Traverse Equipment and Lunar/Mars Surface Systems

    NASA Technical Reports Server (NTRS)

    Mueller, Robert P.; Hoffman, Stephen, J.; Thur, Paul

    2010-01-01

    The parallels between an actual Antarctica South Pole re-supply traverse conducted by the National Science Foundation (NSF) Office of Polar Programs in 2009 have been studied with respect to the latest mission architecture concepts being generated by the United States National Aeronautics and Space Administration (NASA) for lunar and Mars surface systems scenarios. The challenges faced by both endeavors are similar since they must both deliver equipment and supplies to support operations in an extreme environment with little margin for error in order to be successful. By carefully and closely monitoring the manifesting and operational support equipment lists which will enable this South Pole traverse, functional areas have been identified. The equipment required to support these functions will be listed with relevant properties such as mass, volume, spare parts and maintenance schedules. This equipment will be compared to space systems currently in use and projected to be required to support equivalent and parallel functions in Lunar and Mars missions in order to provide a level of realistic benchmarking. Space operations have historically required significant amounts of support equipment and tools to operate and maintain the space systems that are the primary focus of the mission. By gaining insight and expertise in Antarctic South Pole traverses, space missions can use the experience gained over the last half century of Antarctic operations in order to design for operations, maintenance, dual use, robustness and safety which will result in a more cost effective, user friendly, and lower risk surface system on the Moon and Mars. It is anticipated that the U.S Antarctic Program (USAP) will also realize benefits for this interaction with NASA in at least two areas: an understanding of how NASA plans and carries out its missions and possible improved efficiency through factors such as weight savings, alternative technologies, or modifications in training and

  3. ISAARE: Information System for Adaptive, Assistive, and Recreational Equipment: Volume I: Existence; Volume II, Communication; Volume V, Adaptation.

    ERIC Educational Resources Information Center

    Melichar, Joseph F.

    Described as part of the Information System for Adaptive, Assistive and Recreational Equipment are equipment items for physically handicapped pupils in the functional areas of existence, equipment and adaptation. Reviewed in the existence section are such items as assistive food containers and container stabilizers, feeder accessories, bowel and…

  4. Power Product Equipment Technician: Outboard-Engine Boat Systems. Teacher Edition [and] Student Edition.

    ERIC Educational Resources Information Center

    Hilley, Robert

    This curriculum guide contains teacher and student materials for a course on outboard-engine boat systems for power product equipment technician occupations. The course contains the following three units of instruction: (1) Orientation to Outboard-Engine Boat Systems and Rigging; (2) Trailers and Safe Towing and Boat Operation; and (3) Seasonal…

  5. Automated solid-phase extraction coupled online with HPLC-FLD for the quantification of zearalenone in edible oil.

    PubMed

    Drzymala, Sarah S; Weiz, Stefan; Heinze, Julia; Marten, Silvia; Prinz, Carsten; Zimathies, Annett; Garbe, Leif-Alexander; Koch, Matthias

    2015-05-01

    Established maximum levels for the mycotoxin zearalenone (ZEN) in edible oil require monitoring by reliable analytical methods. Therefore, an automated SPE-HPLC online system based on dynamic covalent hydrazine chemistry has been developed. The SPE step comprises a reversible hydrazone formation by ZEN and a hydrazine moiety covalently attached to a solid phase. Seven hydrazine materials with different properties regarding the resin backbone, pore size, particle size, specific surface area, and loading have been evaluated. As a result, a hydrazine-functionalized silica gel was chosen. The final automated online method was validated and applied to the analysis of three maize germ oil samples including a provisionally certified reference material. Important performance criteria for the recovery (70-120 %) and precision (RSDr <25 %) as set by the Commission Regulation EC 401/2006 were fulfilled: The mean recovery was 78 % and RSDr did not exceed 8 %. The results of the SPE-HPLC online method were further compared to results obtained by liquid-liquid extraction with stable isotope dilution analysis LC-MS/MS and found to be in good agreement. The developed SPE-HPLC online system with fluorescence detection allows a reliable, accurate, and sensitive quantification (limit of quantification, 30 μg/kg) of ZEN in edible oils while significantly reducing the workload. To our knowledge, this is the first report on an automated SPE-HPLC method based on a covalent SPE approach.

  6. Instrumentation and control systems, equipment location; instrumentation and control building, ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    Instrumentation and control systems, equipment location; instrumentation and control building, instrumentation room, bays and console plan. Specifications No. Eng-04-353-55-72; drawing no. 60-09-12; sheet 110 of 148; file no. 1321/61. Stamped: Record drawing - as constructed. Below stamp: Contract no. 4338, no change. - Edwards Air Force Base, Air Force Rocket Propulsion Laboratory, Control Center, Test Area 1-115, near Altair & Saturn Boulevards, Boron, Kern County, CA

  7. SCADA-based Operator Support System for Power Plant Equipment Fault Forecasting

    NASA Astrophysics Data System (ADS)

    Mayadevi, N.; Ushakumari, S. S.; Vinodchandra, S. S.

    2014-12-01

    Power plant equipment must be monitored closely to prevent failures from disrupting plant availability. Online monitoring technology integrated with hybrid forecasting techniques can be used to prevent plant equipment faults. A self learning rule-based expert system is proposed in this paper for fault forecasting in power plants controlled by supervisory control and data acquisition (SCADA) system. Self-learning utilizes associative data mining algorithms on the SCADA history database to form new rules that can dynamically update the knowledge base of the rule-based expert system. In this study, a number of popular associative learning algorithms are considered for rule formation. Data mining results show that the Tertius algorithm is best suited for developing a learning engine for power plants. For real-time monitoring of the plant condition, graphical models are constructed by K-means clustering. To build a time-series forecasting model, a multi layer preceptron (MLP) is used. Once created, the models are updated in the model library to provide an adaptive environment for the proposed system. Graphical user interface (GUI) illustrates the variation of all sensor values affecting a particular alarm/fault, as well as the step-by-step procedure for avoiding critical situations and consequent plant shutdown. The forecasting performance is evaluated by computing the mean absolute error and root mean square error of the predictions.

  8. HOW TO SELECT THE PROPER SECURITY AND EQUIPMENT SURVEILLANCE SYSTEMS TO PROTECT YOUR FACILITIES.

    ERIC Educational Resources Information Center

    Honeywell, Inc., Minneapolis, Minn.

    IN PRESENTING A SURVEY OF MODERN SECURITY SYSTEMS, THIS BOOKLET DISCUSSES THE REQUIREMENTS FOR SURVEILLANCE AND PROTECTION OF AREAS, PERIMETER, AND OBJECTS. A VARIETY OF EQUIPMENT IS DESCRIBED WITH DISCUSSION OF OPERATING PROCEDURES, COSTS, AND RECENT DEVELOPMENTS IN SECURITY SYSTEMS. (JT)

  9. Simultaneous analysis of eight bioactive compounds in Danning tablet by HPLC-ESI-MS and HPLC-UV.

    PubMed

    Liu, Runhui; Zhang, Jiye; Liang, Mingjin; Zhang, Weidong; Yan, Shikai; Lin, Min

    2007-02-19

    A high performance liquid chromatography (HPLC) coupled with electrospray tandem mass spectrometry (ESI-MS) and ultraviolet detector (UV) has been developed for the simultaneous analysis of eight bioactive compounds in Danning tablet (including hyperin, hesperidin, resveratrol, nobiletin, curcumine, emodin, chrysophanol, and physcion), a widely used prescription of traditional Chinese medicine (TCM). The chromatographic separation was performed on a ZORBAX Extend C(18) analytical column by gradient elution with acetonitrile and formate buffer (containing 0.05% formic acid, adjusted with triethylamine to pH 5.0) at a flow rate of 0.8 ml/min. The eight compounds in Danning tablet were identified and their MS(n) fractions were elucidated by using HPLC-ESI-MS, and the contents of these compounds were determined by using HPLC-UV method. The standard calibration curves were linear between 5.0 and 100 microg/ml for hyperin, 10-200 microg/ml for hesperidin, 1.0-150 microg/ml for resveratrol, 2.0-120 microg/ml for nobiletin, 2.0-225 microg/ml for curcumine, 20-300 microg/ml for emodin, 2.0-200 microg/ml for chrysophanol, and 20-250 microg/ml for physcion with regression coefficient r(2)>0.9995. The intra-day and inter-day precisions of this method were evaluated with the R.S.D. values less than 0.7% and 1.3%, respectively. The recoveries of the eight investigated compounds were ranged from 99.3% to 100.2% with R.S.D. values less than 1.5%. This method was successfully used to determine the 8 target compounds in 10 batches of Danning tablet.

  10. Complex of technologies and prototype systems for eco-friendly shutdown of the power-generating, process, capacitive, and transport equipment

    NASA Astrophysics Data System (ADS)

    Smorodin, A. I.; Red'kin, V. V.; Frolov, Y. D.; Korobkov, A. A.; Kemaev, O. V.; Kulik, M. V.; Shabalin, O. V.

    2015-07-01

    A set of technologies and prototype systems for eco-friendly shutdown of the power-generating, process, capacitive, and transport equipment is offered. The following technologies are regarded as core technologies for the complex: cryogenic technology nitrogen for displacement of hydrogen from the cooling circuit of turbine generators, cryo blasting of the power units by dioxide granules, preservation of the shutdown power units by dehydrated air, and dismantling and severing of equipment and structural materials of power units. Four prototype systems for eco-friendly shutdown of the power units may be built on the basis of selected technologies: Multimode nitrogen cryogenic system with four subsystems, cryo blasting system with CO2 granules for thermal-mechanical and electrical equipment of power units, and compressionless air-drainage systems for drying and storage of the shutdown power units and cryo-gas system for general severing of the steam-turbine power units. Results of the research and pilot and demonstration tests of the operational units of the considered technological systems allow applying the proposed technologies and systems in the prototype systems for shutdown of the power-generating, process, capacitive, and transport equipment.

  11. 40 CFR 265.32 - Required equipment.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... equipment, or automatic sprinklers, or water spray systems. ... of equipment specified below: (a) An internal communications or alarm system capable of providing... assistance from local police departments, fire departments, or State or local emergency response teams; (c...

  12. 40 CFR 265.32 - Required equipment.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... equipment, or automatic sprinklers, or water spray systems. ... of equipment specified below: (a) An internal communications or alarm system capable of providing... assistance from local police departments, fire departments, or State or local emergency response teams; (c...

  13. 40 CFR 265.32 - Required equipment.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... equipment, or automatic sprinklers, or water spray systems. ... of equipment specified below: (a) An internal communications or alarm system capable of providing... assistance from local police departments, fire departments, or State or local emergency response teams; (c...

  14. 40 CFR 265.32 - Required equipment.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... equipment, or automatic sprinklers, or water spray systems. ... of equipment specified below: (a) An internal communications or alarm system capable of providing... assistance from local police departments, fire departments, or State or local emergency response teams; (c...

  15. 40 CFR 265.32 - Required equipment.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... equipment, or automatic sprinklers, or water spray systems. ... of equipment specified below: (a) An internal communications or alarm system capable of providing... assistance from local police departments, fire departments, or State or local emergency response teams; (c...

  16. [HPLC specific chromatogram of Dendrobium officinale].

    PubMed

    Yan, Mei-Qiu; Chen, Su-Hong; Lv, Gui-Yuan; Zhou, Gui-Fen; Liu, Xia

    2013-02-01

    To establish the method of specific chromatogram analysis of ether extract of Dendrobium officinale for identification of D. officinale. Chromatographic separation was carried out at 30 degrees C on an Ultimate C18 column (4.6 mm x 250 mm, 5 microm) eluted with methanol and water containing 0.2% phosphoric acid in a gradient elution at a flow rate of 1.0 mL x min(-1). The detection wavelength was set at 280 nm. The similarity evaluation system for chromatographic fingerprint of NPC (National Pharmacopoeia Committee) was adopted to specific chromatogram construction. The HPLC specific chromatogram of D. officinale was constructed with 6 common specific chromatographic peaks including naringenin as a reference peak. The method shows good precision and repeatability of relative retention time. It can be used to identify D. officinale.

  17. Simultaneous Speciation of Arsenic, Selenium, and Chromium by HPLC-ICP-MS

    USGS Publications Warehouse

    Wolf, Ruth E.; Morman, Suzette A.; Morrison, Jean M.; Lamothe, Paul J.

    2008-01-01

    An adaptation of an analytical method developed for chromium speciation has been utilized for the simultaneous determination of As(III), As(V), Se(IV), Se(VI), Cr(III), and Cr(VI) species using high performance liquid chromatography (HPLC) separation with ICP-MS detection. Reduction of interferences for the determination of As, Se, and Cr by ICP-MS is a major consideration for this method. Toward this end, a Dynamic Reaction Cell (DRC) ICP-MS system was used to detect the species eluted from the chromatographic column. A variety of reaction cell gases and conditions may be utilized, and the advantages and limitations of the gases tested to date will be presented and discussed. The separation and detection of the As, Se, and Cr species of interest can be achieved using the same chromatographic conditions in less than 2 minutes by complexing the Cr(III) with EDTA prior to injection on the HPLC column. Practical aspects of simultaneous speciation analysis will be presented and discussed, including issues with HPLC sample vial contamination, standard and sample contamination, species stability, and considerations regarding sample collection and preservation methods. The results of testing to determine the method's robustness to common concomitant element and anion effects will also be discussed. Finally, results will be presented using the method for the analysis of a variety of environmental and geological samples including waters, soil leachates and simulated bio-fluid leachates.

  18. 46 CFR 113.10-1 - Approved equipment.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 46 Shipping 4 2011-10-01 2011-10-01 false Approved equipment. 113.10-1 Section 113.10-1 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) ELECTRICAL ENGINEERING COMMUNICATION AND ALARM SYSTEMS AND EQUIPMENT Fire and Smoke Detecting and Alarm Systems § 113.10-1 Approved equipment. Each alarm...

  19. 46 CFR 113.10-1 - Approved equipment.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 46 Shipping 4 2010-10-01 2010-10-01 false Approved equipment. 113.10-1 Section 113.10-1 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) ELECTRICAL ENGINEERING COMMUNICATION AND ALARM SYSTEMS AND EQUIPMENT Fire and Smoke Detecting and Alarm Systems § 113.10-1 Approved equipment. Each alarm...

  20. 46 CFR 113.10-1 - Approved equipment.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 46 Shipping 4 2013-10-01 2013-10-01 false Approved equipment. 113.10-1 Section 113.10-1 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) ELECTRICAL ENGINEERING COMMUNICATION AND ALARM SYSTEMS AND EQUIPMENT Fire and Smoke Detecting and Alarm Systems § 113.10-1 Approved equipment. Each alarm...

  1. 46 CFR 113.10-1 - Approved equipment.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 46 Shipping 4 2014-10-01 2014-10-01 false Approved equipment. 113.10-1 Section 113.10-1 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) ELECTRICAL ENGINEERING COMMUNICATION AND ALARM SYSTEMS AND EQUIPMENT Fire and Smoke Detecting and Alarm Systems § 113.10-1 Approved equipment. Each alarm...

  2. 46 CFR 113.10-1 - Approved equipment.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 46 Shipping 4 2012-10-01 2012-10-01 false Approved equipment. 113.10-1 Section 113.10-1 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) ELECTRICAL ENGINEERING COMMUNICATION AND ALARM SYSTEMS AND EQUIPMENT Fire and Smoke Detecting and Alarm Systems § 113.10-1 Approved equipment. Each alarm...

  3. Size exclusion HPLC of proteins for evaluation of durum wheat quality

    USDA-ARS?s Scientific Manuscript database

    The present research aimed to assess size exclusion HPLC (SE-HPLC) in protein molecular weight distribution determination for quality evaluation of durum semolina. Semolina samples were milled from 13 durum genotypes grown at 7 locations in 2009 and 2010 in ND. Sodium dodecyl sulfate (SDS) buffer ...

  4. Chemical Differentiation of Dendrobium officinale and Dendrobium devonianum by Using HPLC Fingerprints, HPLC-ESI-MS, and HPTLC Analyses

    PubMed Central

    Ye, Zi; Dai, Jia-Rong; Zhang, Cheng-Gang; Lu, Ye; Wu, Lei-Lei; Gong, Amy G. W.; Wang, Zheng-Tao

    2017-01-01

    The stems of Dendrobium officinale Kimura et Migo (Dendrobii Officinalis Caulis) have a high medicinal value as a traditional Chinese medicine (TCM). Because of the limited supply, D. officinale is a high priced TCM, and therefore adulterants are commonly found in the herbal market. The dried stems of a closely related Dendrobium species, Dendrobium devonianum Paxt., are commonly used as the substitute; however, there is no effective method to distinguish the two Dendrobium species. Here, a high performance liquid chromatography (HPLC) method was successfully developed and applied to differentiate D. officinale and D. devonianum by comparing the chromatograms according to the characteristic peaks. A HPLC coupled with electrospray ionization multistage mass spectrometry (HPLC-ESI-MS) method was further applied for structural elucidation of 15 flavonoids, 5 phenolic acids, and 1 lignan in D. officinale. Among these flavonoids, 4 flavonoid C-glycosides were firstly reported in D. officinale, and violanthin and isoviolanthin were identified to be specific for D. officinale compared with D. devonianum. Then, two representative components were used as chemical markers. A rapid and reliable high performance thin layer chromatography (HPTLC) method was applied in distinguishing D. officinale from D. devonianum. The results of this work have demonstrated that these developed analytical methods can be used to discriminate D. officinale and D. devonianum effectively and conveniently. PMID:28769988

  5. Technical standing order : airborne supplemental navigation equipment using the global positioning system (GPS)

    DOT National Transportation Integrated Search

    2001-01-01

    This technical standard order (TSO) prescribes the minimum performance standard that airborne supplemental area navigation equipment using the global positioning system (GPS) must meet in order to be identified with the applicable TSO marking. Airbor...

  6. 11. "INSTRUMENTATION AND CONTROL SYSTEMS, EQUIPMENT LOCATION, TEST STAND TERMINAL ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    11. "INSTRUMENTATION AND CONTROL SYSTEMS, EQUIPMENT LOCATION, TEST STAND TERMINAL ROOM, PLANS AND SECTION." Specifications No. ENG-04-353-55-72; Drawing No. 60-0912; sheet 106 of 148; file no. 1321/57. Stamped: RECORD DRAWING - AS CONSTRUCTED. Below stamp: Contract no. 4338, no change. - Edwards Air Force Base, Air Force Rocket Propulsion Laboratory, Test Stand 1-A Terminal Room, Test Area 1-120, north end of Jupiter Boulevard, Boron, Kern County, CA

  7. National Airspace System Plan: Facilities, Equipment, Associated Development and Other Capital Needs

    DTIC Science & Technology

    1989-09-01

    yes include: 0 Air carrier routes and services will reflect a * Having an operating National Airspace System better balance between trip frequency and... balanced workload. " A major improvement and integration of the FAA communications system will be imple- 0 Reliable equipment, which minimizes the stress...them in this will lead to the development of production level balancing sector staffing levels, specifications in FY 97. * Trial flight plan function

  8. 49 CFR 232.609 - Handling of defective equipment with ECP brake systems.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... and appropriate operating and inspection personnel; and (3) An electronic or written record of the... operating freight cars equipped with ECP brake systems shall adopt and comply with specific procedures... this subpart. These procedures shall be made available to FRA upon request. (2) Each railroad operating...

  9. Improving the Repair Planning System for Mining Equipment on the Basis of Non-destructive Evaluation Data

    NASA Astrophysics Data System (ADS)

    Drygin, Michael; Kuryshkin, Nicholas

    2017-11-01

    The article tells about forming a new concept of scheduled preventive repair system of the equipment at coal mining enterprises, based on the use of modem non-destructive evaluation methods. The approach to the solution for this task is based on the system-oriented analysis of the regulatory documentation, non-destructive evaluation methods and means, experimental studies with compilation of statistics and subsequent grapho-analytical analysis. The main result of the work is a feasible explanation of using non-destructive evaluation methods within the current scheduled preventive repair system, their high efficiency and the potential of gradual transition to condition-based maintenance. In practice wide use of nondestructive evaluation means w;ill allow to reduce significantly the number of equipment failures and to repair only the nodes in pre-accident condition. Considering the import phase-out policy, the solution for this task will allow to adapt the SPR system to Russian market economy conditions and give the opportunity of commercial move by reducing the expenses for maintenance of Russian-made and imported equipment.

  10. Coral Pigments: Quantification Using HPLC and Detection by Remote Sensing

    NASA Technical Reports Server (NTRS)

    Cottone, Mary C.

    1995-01-01

    Widespread coral bleaching (loss of pigments of symbiotic dinoflagellates), and the corresponding decline in coral reef health worldwide, mandates the monitoring of coral pigmentation. Samples of the corals Porites compressa and P. lobata were collected from a healthy reef at Puako, Hawaii, and chlorophyll (chl) a, peridinin, and Beta-carotene (Beta-car) were quantified using reverse-phase high performance liquid chromatography (HPLC). Detailed procedures are presented for the extraction of the coral pigments in 90% acetone, and the separation, identification, and quantification of the major zooxanthellar pigments using spectrophotometry and a modification of the HPLC system described by Mantoura and Llewellyn (1983). Beta-apo-8-carotenal was found to be inadequate as in internal standard, due to coelution with chl b and/or chl a allomer in the sample extracts. Improvements are suggested, which may result in better resolution of the major pigments and greater accuracy in quantification. Average concentrations of peridinin, chl a, and Beta-car in corals on the reef were 5.01, 8.59, and 0.29, micro-grams/cm(exp 2), respectively. Average concentrations of peridinin and Beta-car did not differ significantly between the two coral species sampled; however, the mean chl a concentration in P. compressa specimens (7.81 ,micro-grams/cm(exp 2) was significantly lower than that in P. lobata specimens (9.96 11g/cm2). Chl a concentrations determined spectrophotometrically were significantly higher than those generated through HPLC, suggesting that spectrophotometry overestimates chl a concentrations. The average ratio of chl a-to-peridinin concentrations was 1.90, with a large (53%) coefficient of variation and a significant difference between the two species sampled. Additional data are needed before conclusions can be drawn regarding average pigment concentrations in healthy corals and the consistency of the chl a/peridinin ratio. The HPLC pigment concentration values

  11. User Guide: How to Use and Operate Virtual Reality Equipment in the Systems Assessment and Usability Laboratory (SAUL) for Conducting Demonstrations

    DTIC Science & Technology

    2017-08-01

    ARL-TN-0839 ● AUG 2017 US Army Research Laboratory User Guide: How to Use and Operate Virtual Reality Equipment in the Systems...ARL-TN-0839 ● AUG 2017 US Army Research Laboratory User Guide: How to Use and Operate Virtual Reality Equipment in the Systems...September 2017 4. TITLE AND SUBTITLE User Guide: How to Use and Operate Virtual Reality Equipment in the Systems Assessment and Usability Laboratory

  12. The cytology, isozyme, HPLC fingerprint, and interspecific hybridization studies of genus epimedium (berberidaceae).

    PubMed

    Wang, Lin-Jiao; Sheng, Mao-Yin

    2013-01-01

    104 samples from 27 accessions belonging to 12 species of genus Epimedium were studied on the basis of cytology observation, POD (i.e., peroxide) isozyme, high performance liquid chromatography (i.e., HPLC) fingerprint, and interspecific hybridization. The cytology observation showed karyotypes of twelve species studied; all are 2A symmetry type of Stebbins standard and similar to each other, and except for karyotype of E. leptorrhizum which is 2n = 2x = 8m (2SAT) + 4sm, the rest are 2n = 2x = 6m (2SAT) + 6sm. Chromosomes C-banding of barrenwort species varies, with 15 to 22 bands, consisting of centromeric bands, intercalary bands, terminal bands, and middle satellite bands. Results of POD isozyme showed that the zymographs vary greatly and sixteen bands were detected in the eleven species, and each species has its own characteristic bands different from the others. Studies on the HPLC fingerprint showed that the HPLC fingerprint of different species has characteristic peaks, divided into two regions (retention time < 10 min and retention time > 10 min). Results of interspecific hybridization showed that crosses of any combination among seven species studied are successful and the rates of grain set vary greatly. Based on these results, the system and phylogeny of this genus were inferred.

  13. 40 CFR 60.4345 - What are the requirements for the continuous emission monitoring system equipment, if I choose to...

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... continuous emission monitoring system equipment, if I choose to use this option? 60.4345 Section 60.4345... PERFORMANCE FOR NEW STATIONARY SOURCES Standards of Performance for Stationary Combustion Turbines Monitoring § 60.4345 What are the requirements for the continuous emission monitoring system equipment, if I...

  14. 40 CFR 60.4345 - What are the requirements for the continuous emission monitoring system equipment, if I choose to...

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... continuous emission monitoring system equipment, if I choose to use this option? 60.4345 Section 60.4345... PERFORMANCE FOR NEW STATIONARY SOURCES Standards of Performance for Stationary Combustion Turbines Monitoring § 60.4345 What are the requirements for the continuous emission monitoring system equipment, if I...

  15. [Determination of azoxystrobin in tea by HPLC].

    PubMed

    Chonan, T

    2001-08-01

    A determination method has been developed for azoxystrobin in tea by HPLC. Azoxystrobin was extracted from a sample with acetone, and the extract was passed through an alumina column to remove tannin. The eluate was concentrated to ca. 25 mL and passed through a Sep-Pak Vac tC18 to remove pigments. The eluate was cleaned-up by using liquid-liquid partition, and Florisil and silica-gel columns. The HPLC analysis for azoxystrobin was carried out on a C18 column with acetonitrile-water (9:11) as the mobile phase, with ultraviolet detection at 260 nm. The recovery of azoxystrobin fortified at the level of 0.4 microgram/g was 90.2% and the limit of determination was 0.2 microgram/g.

  16. Cycle chemistry monitoring system as means of improving the reliability of the equipment at the power plants

    NASA Astrophysics Data System (ADS)

    Yegoshina, O. V.; Voronov, V. N.; Yarovoy, V. O.; Bolshakova, N. A.

    2017-11-01

    There are many problems in domestic energy at the present that require urgent solutions in the near future. One of these problems - the aging of the main and auxiliary equipment. Wear of equipment is the cause of decrease reliability and efficiency of power plants. Reliability of the equipment are associated with the introduction of cycle chemistry monitoring system. The most damageable equipment’s are boilers (52.2 %), turbines (12.6 %) and heating systems (12.3 %) according to the review of failure rate on the power plants. The most part of the damageability of the boiler is heated surfaces (73.2 %). According to the Russian technical requirements, the monitoring systems are responsible to reduce damageability the boiler heating surfaces and to increase the reliability of the equipment. All power units capacity of over 50 MW are equipped with cycle chemistry monitoring systems in order to maintain water chemistry within operating limits. The main idea of cycle chemistry monitoring systems is to improve water chemistry at power plants. According to the guidelines, cycle chemistry monitoring systems of a single unit depends on its type (drum or once-through boiler) and consists of: 20…50 parameters of on-line chemical analyzers; 20…30 «grab» sample analyses (daily) and about 15…20 on-line monitored operating parameters. The operator of modern power plant uses with many data at different points of steam/water cycle. Operators do not can estimate quality of the cycle chemistry due to the large volume of daily and every shift information and dispersion of data, lack of systematization. In this paper, an algorithm for calculating the quality index developed for improving control the water chemistry of the condensate, feed water and prevent scaling and corrosion in the steam/water cycle.

  17. Validation of blood vitamin A concentrations in cattle: comparison of a new cow-side test (iCheck™ FLUORO) with high-performance liquid chromatography (HPLC).

    PubMed

    Raila, Jens; Kawashima, Chiho; Sauerwein, Helga; Hülsmann, Nadine; Knorr, Christoph; Myamoto, Akio; Schweigert, Florian J

    2017-05-10

    Plasma concentration of retinol is an accepted indicator to assess the vitamin A (retinol) status in cattle. However, the determination of vitamin A requires a time consuming multi-step procedure, which needs specific equipment to perform extraction, centrifugation or saponification prior to high-performance liquid chromatography (HPLC). The concentrations of retinol in whole blood (n = 10), plasma (n = 132) and serum (n = 61) were measured by a new rapid cow-side test (iCheck™ FLUORO) and compared with those by HPLC in two independent laboratories in Germany (DE) and Japan (JP). Retinol concentrations in plasma ranged from 0.033 to 0.532 mg/L, and in serum from 0.043 to 0.360 mg/L (HPLC method). No significant differences in retinol levels were observed between the new rapid cow-side test and HPLC performed in different laboratories (HPLC vs. iCheck™ FLUORO: 0.320 ± 0.047 mg/L vs. 0.333 ± 0.044 mg/L, and 0.240 ± 0.096 mg/L vs. 0.241 ± 0.069 mg/L, lab DE and lab JP, respectively). A similar comparability was observed when whole blood was used (HPLC vs. iCheck™ FLUORO: 0.353 ± 0.084 mg/L vs. 0.341 ± 0.064 mg/L). Results showed a good agreement between both methods based on correlation coefficients of r 2  = 0.87 (P < 0.001) and Bland-Altman blots revealed no significant bias for all comparison. With the new rapid cow-side test (iCheck™ FLUORO) retinol concentrations in cattle can be reliably assessed within a few minutes and directly in the barn using even whole blood without the necessity of prior centrifugation. The ease of the application of the new rapid cow-side test and its portability can improve the diagnostic of vitamin A status and will help to control vitamin A supplementation in specific vitamin A feeding regimes such as used to optimize health status in calves or meat marbling in Japanese Black cattle.

  18. An Analysis of the Crash Experience of Vehicles Equipped with Antilock Braking System

    DOT National Transportation Integrated Search

    1995-06-01

    National Center for Statistics and Analysis has recently completed an initial : analysis of the crash experience of passenger cars (PCs) and light trucks and : vans (LTVs) equipped with antilock braking systems (ABS). Four types of crashes : were ide...

  19. The Architecture Design of Detection and Calibration System for High-voltage Electrical Equipment

    NASA Astrophysics Data System (ADS)

    Ma, Y.; Lin, Y.; Yang, Y.; Gu, Ch; Yang, F.; Zou, L. D.

    2018-01-01

    With the construction of Material Quality Inspection Center of Shandong electric power company, Electric Power Research Institute takes on more jobs on quality analysis and laboratory calibration for high-voltage electrical equipment, and informationization construction becomes urgent. In the paper we design a consolidated system, which implements the electronic management and online automation process for material sampling, test apparatus detection and field test. In the three jobs we use QR code scanning, online Word editing and electronic signature. These techniques simplify the complex process of warehouse management and testing report transferring, and largely reduce the manual procedure. The construction of the standardized detection information platform realizes the integrated management of high-voltage electrical equipment from their networking, running to periodic detection. According to system operation evaluation, the speed of transferring report is doubled, and querying data is also easier and faster.

  20. Orbiter electrical equipment utilization baseline

    NASA Technical Reports Server (NTRS)

    1980-01-01

    The baseline for utilization of Orbiter electrical equipment in both electrical and Environmental Control and Life Support System (ECLSS) thermal analyses is established. It is a composite catalog of Space Shuttle equipment, as defined in the Shuttle Operational Data Book. The major functions and expected usage of each component type are described. Functional descriptions are designed to provide a fundamental understanding of the Orbiter electrical equipment, to insure correlation of equipment usage within nominal analyses, and to aid analysts in the formulation of off-nominal, contingency analyses.

  1. 14 CFR 29.1431 - Electronic equipment.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 14 Aeronautics and Space 1 2014-01-01 2014-01-01 false Electronic equipment. 29.1431 Section 29... AIRWORTHINESS STANDARDS: TRANSPORT CATEGORY ROTORCRAFT Equipment Miscellaneous Equipment § 29.1431 Electronic... operation of any other radio or electronic unit, or system of units, required by this chapter. ...

  2. 14 CFR 29.1431 - Electronic equipment.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 14 Aeronautics and Space 1 2013-01-01 2013-01-01 false Electronic equipment. 29.1431 Section 29... AIRWORTHINESS STANDARDS: TRANSPORT CATEGORY ROTORCRAFT Equipment Miscellaneous Equipment § 29.1431 Electronic... operation of any other radio or electronic unit, or system of units, required by this chapter. ...

  3. 14 CFR 29.1431 - Electronic equipment.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 14 Aeronautics and Space 1 2012-01-01 2012-01-01 false Electronic equipment. 29.1431 Section 29... AIRWORTHINESS STANDARDS: TRANSPORT CATEGORY ROTORCRAFT Equipment Miscellaneous Equipment § 29.1431 Electronic... operation of any other radio or electronic unit, or system of units, required by this chapter. ...

  4. 14 CFR 29.1431 - Electronic equipment.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 14 Aeronautics and Space 1 2011-01-01 2011-01-01 false Electronic equipment. 29.1431 Section 29... AIRWORTHINESS STANDARDS: TRANSPORT CATEGORY ROTORCRAFT Equipment Miscellaneous Equipment § 29.1431 Electronic... operation of any other radio or electronic unit, or system of units, required by this chapter. ...

  5. 46 CFR 184.210 - Heating equipment.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 46 Shipping 7 2010-10-01 2010-10-01 false Heating equipment. 184.210 Section 184.210 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) SMALL PASSENGER VESSELS (UNDER 100 GROSS TONS) VESSEL CONTROL AND MISCELLANEOUS SYSTEMS AND EQUIPMENT Cooking and Heating § 184.210 Heating equipment...

  6. 40 CFR 51.358 - Test equipment.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 40 Protection of Environment 2 2014-07-01 2014-07-01 false Test equipment. 51.358 Section 51.358....358 Test equipment. Computerized emission test systems are required for performing an official emissions test on subject vehicles. (a) Performance features of computerized emission test systems. The...

  7. 40 CFR 51.358 - Test equipment.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 40 Protection of Environment 2 2010-07-01 2010-07-01 false Test equipment. 51.358 Section 51.358....358 Test equipment. Computerized emission test systems are required for performing an official emissions test on subject vehicles. (a) Performance features of computerized emission test systems. The...

  8. 76 FR 79755 - First Meeting: RTCA Special Committee 226 Audio Systems and Equipment

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-12-22

    ... Administrative Remarks Introductions RTCA Overview Audio Systems and Equipment--Background and History Agenda..., Discussion, Recommendations and Assignment of Responsibilities Other Business Establish Agenda for Next..., Manager, Business Operations Branch, Federal Aviation Administration. [FR Doc. 2011-32863 Filed 12-21-11...

  9. 14 CFR 25.1353 - Electrical equipment and installations.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 14 Aeronautics and Space 1 2011-01-01 2011-01-01 false Electrical equipment and installations. 25... TRANSPORTATION AIRCRAFT AIRWORTHINESS STANDARDS: TRANSPORT CATEGORY AIRPLANES Equipment Electrical Systems and Equipment § 25.1353 Electrical equipment and installations. (a) Electrical equipment and controls must be...

  10. 14 CFR 25.1353 - Electrical equipment and installations.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 14 Aeronautics and Space 1 2012-01-01 2012-01-01 false Electrical equipment and installations. 25... TRANSPORTATION AIRCRAFT AIRWORTHINESS STANDARDS: TRANSPORT CATEGORY AIRPLANES Equipment Electrical Systems and Equipment § 25.1353 Electrical equipment and installations. (a) Electrical equipment and controls must be...

  11. 14 CFR 25.1353 - Electrical equipment and installations.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 14 Aeronautics and Space 1 2013-01-01 2013-01-01 false Electrical equipment and installations. 25... TRANSPORTATION AIRCRAFT AIRWORTHINESS STANDARDS: TRANSPORT CATEGORY AIRPLANES Equipment Electrical Systems and Equipment § 25.1353 Electrical equipment and installations. (a) Electrical equipment and controls must be...

  12. 14 CFR 29.1353 - Electrical equipment and installations.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 14 Aeronautics and Space 1 2012-01-01 2012-01-01 false Electrical equipment and installations. 29... TRANSPORTATION AIRCRAFT AIRWORTHINESS STANDARDS: TRANSPORT CATEGORY ROTORCRAFT Equipment Electrical Systems and Equipment § 29.1353 Electrical equipment and installations. (a) Electrical equipment, controls, and wiring...

  13. 14 CFR 29.1353 - Electrical equipment and installations.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 14 Aeronautics and Space 1 2014-01-01 2014-01-01 false Electrical equipment and installations. 29... TRANSPORTATION AIRCRAFT AIRWORTHINESS STANDARDS: TRANSPORT CATEGORY ROTORCRAFT Equipment Electrical Systems and Equipment § 29.1353 Electrical equipment and installations. (a) Electrical equipment, controls, and wiring...

  14. 14 CFR 25.1353 - Electrical equipment and installations.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 14 Aeronautics and Space 1 2014-01-01 2014-01-01 false Electrical equipment and installations. 25... TRANSPORTATION AIRCRAFT AIRWORTHINESS STANDARDS: TRANSPORT CATEGORY AIRPLANES Equipment Electrical Systems and Equipment § 25.1353 Electrical equipment and installations. (a) Electrical equipment and controls must be...

  15. 14 CFR 29.1353 - Electrical equipment and installations.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 14 Aeronautics and Space 1 2013-01-01 2013-01-01 false Electrical equipment and installations. 29... TRANSPORTATION AIRCRAFT AIRWORTHINESS STANDARDS: TRANSPORT CATEGORY ROTORCRAFT Equipment Electrical Systems and Equipment § 29.1353 Electrical equipment and installations. (a) Electrical equipment, controls, and wiring...

  16. Management methodology for pressure equipment

    NASA Astrophysics Data System (ADS)

    Bletchly, P. J.

    Pressure equipment constitutes a significant investment in capital and a major proportion of potential high-risk plant in many operations and this is particularly so in an alumina refinery. In many jurisdictions pressure equipment is also subject to statutory regulation that imposes obligations on Owners of the equipment with respect to workplace safety. Most modern technical standards and industry codes of practice employ a risk-based approach to support better decision making with respect to pressure equipment. For a management system to be effective it must demonstrate that risk is being managed within acceptable limits.

  17. 46 CFR 184.220 - Cooking equipment.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 46 Shipping 7 2010-10-01 2010-10-01 false Cooking equipment. 184.220 Section 184.220 Shipping...) VESSEL CONTROL AND MISCELLANEOUS SYSTEMS AND EQUIPMENT Cooking and Heating § 184.220 Cooking equipment. (a) Doors on a cooking appliance must be provided with hinges and locking devices to prevent...

  18. [Study on HPLC fingerprint of 11 Taraxacum species in northeast of China].

    PubMed

    Zhu, Dan; Zhao, Xin; Xu, Qiao; Ning, Wei

    2011-04-01

    To study the RP-HPLC fingerprints of 11 plants in the genus Taraxacum for their quality control. The fingerprints were determined using an Agilent 1100 series instrument system. Chromatographic analyses were performed on a Kromasil 100-5 C18 (4.6 mm x 250 mm, 5 microm) analytical column,eluted with methanol and water containing 0.5% acetic acid as the mobile phases in gradient elution at the flow rate of 1.0 mL x min(-1). The detection wavelength was 323 nm. The temperature of column was 35 degrees C. Eleven species of Taraxacum in northeast of China were detected respectively. Twenty-five common peaks existed in 11 RP-HPLC fingerprints. By comparing the retention time and the on-line UV spectra, peaks No. 10, No. 12, No. 16 and No. 25 were identified as chlorogenic acid, caffeic acid, p-coumaroy acid and luteolin respectively. The analytical method with good precision and reproducibility can be useful in the quality control of Taraxacum plants.

  19. Power Product Equipment Technician: Outboard-Engine Systems and Service. Teacher Edition [and] Student Edition.

    ERIC Educational Resources Information Center

    Hilley, Robert

    This curriculum guide contains teacher and student materials for a course on outboard-engine boat systems and service for power product equipment technician occupations. The course contains the following four units of instruction: (1) Outboard-Engine Design and Identification; (2) Operation and Service of Engine-Support Systems; (3) Operation and…

  20. Lighting Control System for Premises with Display Screen Equipment

    NASA Astrophysics Data System (ADS)

    Kudryashov, A. V.

    2017-11-01

    The use of Display Screen Equipment (DSE) at enterprises allows one to increase the productivity and safety of production, minimize the number of personnel and leads to the simplification of the work of specialists, but on the other side, changes usual working conditions. If the personnel works with displays, visual fatigue develops more quickly which contributes to the emergence of nervous tension, stress and possible erroneous actions. Low interest of the lighting control system developers towards the rooms with displays is dictated by special requirements for coverage by sanitary and hygienic standards (limiting excess workplace illumination). We decided to create a combined lighting system which works considering daylight illumination and artificial light sources. The brightness adjustment of the LED lamps is carried out according to the DALI protocol, adjustment of the natural illumination by means of smart glasses. The technical requirements for a lighting control system, the structural-functional scheme and the algorithm for controlling the operation of the system have been developed. The elements of control units, sensors and actuators have been selected.

  1. A typewriting system operated by head movements, based on home-computer equipment.

    PubMed

    Heuvelmans, A M; Mélotte, H E; Neve, J J

    1990-06-01

    For persons who cannot move their hands and legs we have designed a relatively inexpensive typewriting system which can be operated by movements of the head. The typewriter is made up of commercially available home computer equipment - i e, a computer including monitor and printer and a headset replacing the mouse. A user-friendly software program has been designed to replace the mouse button and to make this equipment act as a typewriter with simple text-editing features. Some ergonomics aspects of the typewriter set-up and the results of an evaluation of the typewriter by two patients suffering from amyotrophic lateral sclerosis (ALS) are given. Several factors relevant to the design, construction, evaluation and application of the typewriter are discussed.

  2. Design of "Eye Closure" system for the stealth of photo-electric equipments

    NASA Astrophysics Data System (ADS)

    Zhang, Y.; Hua, W. S.; Li, G.

    2012-10-01

    Based on the optical activity of liquid crystal, a new approach for the stealth of "cat's eye" targets is proposed in this paper. It imitates the physiological close reaction of human eyes when strong light irradiates eyes. With this approach, the "cat's eye" effect will vanish, which is applied in restricting photo-electric equipments being detected and located by active laser detection system. The structure and working principle of the design are presented. The drive circuit is given to control the optical switch automatically. Feasibility of this design is demonstrated by experimental method. The measured data illustrate that the proposed approach is effective to eliminate the "cat's eye" effect, so as to enhancing the viability of photo-electric equipments on the battlefield.

  3. HPLC study on the 'history' dependence of gramicidin A conformation in phospholipid model membranes.

    PubMed

    Bañó, M C; Braco, L; Abad, C

    1989-06-19

    A novel HPLC methodology for the study of gramicidin A reconstituted in model membranes has been tested in comparison with circular dichroism data. It is shown that this chromatographic technique not only corroborates most of the recent spectroscopic results but allows one to explain them in terms of mass fractions of different actual conformational species of GA in the phospholipid assemblies. In particular, the dependence of the inserted peptide configuration on the organic solvent and other parameters involved in the 'history' of the sample preparation and handling has been analyzed by HPLC in two phospholipid model systems: small unilamellar vesicles and micelles. Moreover, a slow conformational transition of GA towards a beta 6.3-helical configuration, accelerated by heat incubation, has been also chromatographically visualized and quantitatively interpreted.

  4. Comparison of piracetam measured with HPLC-DAD, HPLC-ESI-MS, DIP-APCI-MS, and a newly developed and optimized DIP-ESI-MS.

    PubMed

    Lenzen, Claudia; Winterfeld, Gottfried A; Schmitz, Oliver J

    2016-06-01

    The direct inlet probe-electrospray ionization (DIP-ESI) presented here was based on the direct inlet probe-atmospheric pressure chemical ionization (DIP-APCI) developed by our group. It was coupled to an ion trap mass spectrometer (MS) for the detection of more polar compounds such as degradation products from pharmaceuticals. First, the position of the ESI tip, the gas and solvent flow rates, as well as the gas temperature were optimized with the help of the statistic program Minitab® 17 and a caffeine standard. The ability to perform quantitative analyses was also tested by using different concentrations of caffeine and camphor. Calibration curves with a quadratic calibration regression of R (2) = 0.9997 and 0.9998 for caffeine and camphor, respectively, were obtained. The limit of detection of 2.5 and 1.7 ng per injection for caffeine and camphor were determined, respectively. Furthermore, a solution of piracetam was used to compare established analytical methods for this drug and its impurities such as HPLC-diode array detector (DAD) and HPLC-ESI-MS with the DIP-APCI and the developed DIP-ESI. With HPLC-DAD and 10 μg piracetam on column, no impurity could be detected. With HPLC-ESI-MS, two impurities (A and B) were identified with only 4.6 μg piracetam on column, while with DIP-ESI, an amount of 1.6 μg piracetam was sufficient. In the case of the DIP-ESI measurements, all detected impurities could be identified by MS/MS studies. Graphical Abstract Scheme of the DIP-ESI principle.

  5. Determination of Bortezomib in API Samples Using HPLC: Assessment of Enantiomeric and Diastereomeric Impurities.

    PubMed

    Kamalzadeh, Zahra; Babanezhad, Esmaeil; Ghaffari, Solmaz; Mohseni Ezhiyeh, Alireza; Mohammadnejad, Mahdieh; Naghibfar, Mehdi; Bararjanian, Morteza; Attar, Hossein

    2017-08-01

    A new, normal phase high performance liquid chromatography (NP-HPLC) method was developed for separation of Bortezomib (BZB) enantiomers and quantitative determination of (1S,2R)-enantiomer of BZB in active pharmaceutical ingredient (API) samples. The developed method was validated based on International Conference on Harmonisation (ICH) guidelines and it was proved to be accurate, precise and robust. The obtained resolution (RS) between the enantiomers was more than 2. The calibration curve for (1S,2R)-enantiomer was found to be linear in the concentration range of 0.24-5.36 mg/L with regression coefficient (R2) of 0.9998. Additionally, the limit of detection (LOD) and limit of quantification (LOQ) were 0.052 and 0.16 mg/L, respectively. Also, in this study, a precise, sensitive and robust gradient reversed-phase HPLC (RP-HPLC) method was developed and validated for determination of BZB in API samples. The detector response was linear over the concentration range of 0.26-1110.5 mg/L. The values of R2, LOD and LOQ were 0.9999, 0.084 and 0.25 mg/L, respectively. For both NP-HPLC and RP-HPLC methods, all of the RSD (%) values obtained in the precision study were <1.0%. System suitability parameters in terms of tailing factor (TF), number of theoretical plates (N) and RS were TF < 2.0, N > 2,000 and RS > 2.0. The performance of two common integration methods of valley to valley and drop perpendicular for drawing the baseline between two adjacent peaks were investigated for the determination of diastereomeric impurity (Imp-D) in the BZB-API samples. The results showed that the valley to valley method outperform the drop perpendicular method for calculation of Imp-D peak areas. Therefore, valley to valley method was chosen for peak integration. © The Author 2017. Published by Oxford University Press. All rights reserved. For Permissions, please email: journals.permissions@oup.com.

  6. Multivariate optimization and validation of an analytical methodology by RP-HPLC for the determination of losartan potassium in capsules.

    PubMed

    Bonfilio, Rudy; Tarley, César Ricardo Teixeira; Pereira, Gislaine Ribeiro; Salgado, Hérida Regina Nunes; de Araújo, Magali Benjamim

    2009-11-15

    This paper describes the optimization and validation of an analytical methodology for the determination of losartan potassium in capsules by HPLC using 2(5-1) fractional factorial and Doehlert designs. This multivariate approach allows a considerable improvement in chromatographic performance using fewer experiments, without additional cost for columns or other equipment. The HPLC method utilized potassium phosphate buffer (pH 6.2; 58 mmol L(-1))-acetonitrile (65:35, v/v) as the mobile phase, pumped at a flow rate of 1.0 mL min(-1). An octylsilane column (100 mm x 4.6mm i.d., 5 microm) maintained at 35 degrees C was used as the stationary phase. UV detection was performed at 254 nm. The method was validated according to the ICH guidelines, showing accuracy, precision (intra-day relative standard deviation (R.S.D.) and inter-day R.S.D values <2.0%), selectivity, robustness and linearity (r=0.9998) over a concentration range from 30 to 70 mg L(-1) of losartan potassium. The limits of detection and quantification were 0.114 and 0.420 mg L(-1), respectively. The validated method may be used to quantify losartan potassium in capsules and to determine the stability of this drug.

  7. Spaceflight Ground Support Equipment Reliability & System Safety Data

    NASA Technical Reports Server (NTRS)

    Fernandez, Rene; Riddlebaugh, Jeffrey; Brinkman, John; Wilkinson, Myron

    2012-01-01

    Presented were Reliability Analysis, consisting primarily of Failure Modes and Effects Analysis (FMEA), and System Safety Analysis, consisting of Preliminary Hazards Analysis (PHA), performed to ensure that the CoNNeCT (Communications, Navigation, and Networking re- Configurable Testbed) Flight System was safely and reliably operated during its Assembly, Integration and Test (AI&T) phase. A tailored approach to the NASA Ground Support Equipment (GSE) standard, NASA-STD-5005C, involving the application of the appropriate Requirements, S&MA discipline expertise, and a Configuration Management system (to retain a record of the analysis and documentation) were presented. Presented were System Block Diagrams of selected GSE and the corresponding FMEA, as well as the PHAs. Also discussed are the specific examples of the FMEAs and PHAs being used during the AI&T phase to drive modifications to the GSE (via "redlining" of test procedures, and the placement of warning stickers to protect the flight hardware) before being interfaced to the Flight System. These modifications were necessary because failure modes and hazards were identified during the analysis that had not been properly mitigated. Strict Configuration Management was applied to changes (whether due to upgrades or expired calibrations) in the GSE by revisiting the FMEAs and PHAs to reflect the latest System Block Diagrams and Bill Of Material. The CoNNeCT flight system has been successfully assembled, integrated, tested, and shipped to the launch site without incident. This demonstrates that the steps taken to safeguard the flight system when it was interfaced to the various GSE were successful.

  8. Modeling and analysis of equipment managers in manufacturing execution systems for semiconductor packaging.

    PubMed

    Cheng, F T; Yang, H C; Luo, T L; Feng, C; Jeng, M

    2000-01-01

    Equipment Managers (EMs) play a major role in a Manufacturing Execution System (MES). They serve as the communication bridge between the components of an MES and the equipment. The purpose of this paper is to propose a novel methodology for developing analytical and simulation models for the EM such that the validity and performance of the EM can be evaluated. Domain knowledge and requirements are collected from a real semiconductor packaging factory. By using IDEFO and state diagrams, a static functional model and a dynamic state model of the EM are built. Next, these two models are translated into a Petri net model. This allows qualitative and quantitative analyses of the system. The EM net model is then expanded into the MES net model. Therefore, the performance of an EM in the MES environment can be evaluated. These evaluation results are good references for design and decision making.

  9. 47 CFR 95.129 - Station equipment.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... SERVICES General Mobile Radio Service (GMRS) § 95.129 Station equipment. Every station in a GMRS system... find out if a particular transmitter has been certificated for the GMRS. All station equipment in a GMRS system must comply with the technical rules in part 95. [63 FR 68975, Dec. 14, 1998] ...

  10. Optimal Medical Equipment Maintenance Service Proposal Decision Support System combining Activity Based Costing (ABC) and the Analytic Hierarchy Process (AHP).

    PubMed

    da Rocha, Leticia; Sloane, Elliot; M Bassani, Jose

    2005-01-01

    This study describes a framework to support the choice of the maintenance service (in-house or third party contract) for each category of medical equipment based on: a) the real medical equipment maintenance management system currently used by the biomedical engineering group of the public health system of the Universidade Estadual de Campinas located in Brazil to control the medical equipment maintenance service, b) the Activity Based Costing (ABC) method, and c) the Analytic Hierarchy Process (AHP) method. Results show the cost and performance related to each type of maintenance service. Decision-makers can use these results to evaluate possible strategies for the categories of equipment.

  11. 2014 Salish Kootenai College Equipment Grant

    DTIC Science & Technology

    2016-09-14

    using an appropriate purification machine. With this appropriate equipment, protein has been purified from a yeast expression system to explore the...purification machine. With this appropriate equipment, protein has been purified from a yeast expression system to explore the effects of that protein on...Scientific Progress We have successfully purified human YKL39 chilectin protein from a yeast expression system. We are currently working on purification

  12. 29 CFR 1926.952 - Mechanical equipment.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... of each shift during which the equipment is to be used to determine that the brakes and operating systems are in proper working condition. (3) No employer shall use any motor vehicle equipment having an... certified for work on the proper voltage, mechanical equipment shall not be operated closer to any energized...

  13. 29 CFR 1926.952 - Mechanical equipment.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... of each shift during which the equipment is to be used to determine that the brakes and operating systems are in proper working condition. (3) No employer shall use any motor vehicle equipment having an... certified for work on the proper voltage, mechanical equipment shall not be operated closer to any energized...

  14. Analysis of peptides using an integrated microchip HPLC-MS/MS system.

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Kirby, Brian J.; Chirica, Gabriela S.; Reichmuth, David S.

    Hyphendated LC-MS techniques are quickly becoming the standard tool for protemic analyses. For large homogeneous samples, bulk processing methods and capillary injection and separation techniques are suitable. However, for analysis of small or heterogeneous samples, techniques that can manipulate picoliter samples without dilution are required or samples will be lost or corrupted; further, static nanospray-type flowrates are required to maximize SNR. Microchip-level integration of sample injection with separation and mass spectrometry allow small-volume analytes to be processed on chip and immediately injected without dilution for analysis. An on-chip HPLC was fabricated using in situ polymerization of both fixed and mobilemore » polymer monoliths. Integration of the chip with a nanospray MS emitter enables identification of peptides by the use of tandem MS. The chip is capable of analyzing of very small sample volumes (< 200 pl) in short times (< 3 min).« less

  15. Multielemental speciation analysis by advanced hyphenated technique - HPLC/ICP-MS: A review.

    PubMed

    Marcinkowska, Monika; Barałkiewicz, Danuta

    2016-12-01

    Speciation analysis has become an invaluable tool in human health risk assessment, environmental monitoring or food quality control. Another step is to develop reliable multielemental speciation methodologies, to reduce costs, waste and time needed for the analysis. Separation and detection of species of several elements in a single analytical run can be accomplished by high performance liquid chromatography hyphenated to inductively coupled plasma mass spectrometry (HPLC/ICP-MS). Our review assembles articles concerning multielemental speciation determination of: As, Se, Cr, Sb, I, Br, Pb, Hg, V, Mo, Te, Tl, Cd and W in environmental, biological, food and clinical samples analyzed with HPLC/ICP-MS. It addresses the procedures in terms of following issues: sample collection and pretreatment, selection of optimal conditions for elements species separation by HPLC and determination using ICP-MS as well as metrological approach. The presented work is the first review article concerning multielemental speciation analysis by advanced hyphenated technique HPLC/ICP-MS. Copyright © 2016 Elsevier B.V. All rights reserved.

  16. The Cytology, Isozyme, HPLC Fingerprint, and Interspecific Hybridization Studies of Genus Epimedium (Berberidaceae)

    PubMed Central

    Wang, Lin-Jiao; Sheng, Mao-Yin

    2013-01-01

    104 samples from 27 accessions belonging to 12 species of genus Epimedium were studied on the basis of cytology observation, POD (i.e., peroxide) isozyme, high performance liquid chromatography (i.e., HPLC) fingerprint, and interspecific hybridization. The cytology observation showed karyotypes of twelve species studied; all are 2A symmetry type of Stebbins standard and similar to each other, and except for karyotype of E. leptorrhizum which is 2n = 2x = 8m (2SAT) + 4sm, the rest are 2n = 2x = 6m (2SAT) + 6sm. Chromosomes C-banding of barrenwort species varies, with 15 to 22 bands, consisting of centromeric bands, intercalary bands, terminal bands, and middle satellite bands. Results of POD isozyme showed that the zymographs vary greatly and sixteen bands were detected in the eleven species, and each species has its own characteristic bands different from the others. Studies on the HPLC fingerprint showed that the HPLC fingerprint of different species has characteristic peaks, divided into two regions (retention time < 10 min and retention time > 10 min). Results of interspecific hybridization showed that crosses of any combination among seven species studied are successful and the rates of grain set vary greatly. Based on these results, the system and phylogeny of this genus were inferred. PMID:24349794

  17. [Study on HPLC fingerprint of flavonoids from Houttuynia cordata by comparing with fingerprint reference].

    PubMed

    Zhang, Ting-Ting; Wu, Yi; Hang, Tai-Jun

    2009-05-01

    To establish a stable and repeatable HPLC fingerprint standard and evaluate the flavonoids from Houttuynia cordata qualitatively and quantitatively. HPLC separation was performed on a C18 column with methanol-0.1% phosphoric acid mixed solution as mobile phase in gradient elution mode. The fingerprint reference was determined as one of the most typical chromatograms and used to be compared with other samples through Cosine and Relative Euclid Distance methods, thus the chromatographic fingerprints of flavonoids from Houttuynia cordata were evaluated by constitutes and contents, respectively. Fourteen mutual peaks were fixed in the HPLC fingerprint of flavonoids from Houttaynia cordata. It showed good results in validation tests in which the quercitrin's peak was set as the reference peak to calculate relative retention time and area of other peaks in the chromatograms, and the RSD were less than 0.2% and 5.0%, respectively. The linear ranges for quercitrin was 1.07-83.4 microg/mL (r=0.9999) and the average recovery was 100.3%. The method shows good repeatability, ruggedness and reliability. Comparing with the established reference fingerprint, the evaluation system including Cosine and Relative Euclid Distance methods lays dependable foundation for controlling the quality of Houttuynia cordata.

  18. 29 CFR 1926.952 - Mechanical equipment.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... of each shift during which the equipment is to be used to determine that the brakes and operating systems are in proper working condition. (3) No employer shall use any motor vehicle equipment having an... shall not be operated closer to any energized line or equipment than the clearances set forth in § 1926...

  19. 46 CFR 121.220 - Cooking equipment.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 46 Shipping 4 2010-10-01 2010-10-01 false Cooking equipment. 121.220 Section 121.220 Shipping... SYSTEMS AND EQUIPMENT Cooking and Heating § 121.220 Cooking equipment. (a) Doors on a cooking appliance... cooking appliance must be installed to prevent movement in heavy seas. (c) For a grill or similar type of...

  20. 14 CFR 25.1307 - Miscellaneous equipment.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... AIRWORTHINESS STANDARDS: TRANSPORT CATEGORY AIRPLANES Equipment General § 25.1307 Miscellaneous equipment. The... electrical energy. (c) Electrical protective devices, as prescribed in this part. (d) Two systems for two-way...

  1. 2 CFR 200.313 - Equipment.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... awarding agencies. This includes consolidated equipment for information technology systems. (2) During the... and Agreements Office of Management and Budget Guidance for Grants and Agreements OFFICE OF MANAGEMENT... subject to the following conditions: (1) Use the equipment for the authorized purposes of the project...

  2. Common data buffer system. [communication with computational equipment utilized in spacecraft operations

    NASA Technical Reports Server (NTRS)

    Byrne, F. (Inventor)

    1981-01-01

    A high speed common data buffer system is described for providing an interface and communications medium between a plurality of computers utilized in a distributed computer complex forming part of a checkout, command and control system for space vehicles and associated ground support equipment. The system includes the capability for temporarily storing data to be transferred between computers, for transferring a plurality of interrupts between computers, for monitoring and recording these transfers, and for correcting errors incurred in these transfers. Validity checks are made on each transfer and appropriate error notification is given to the computer associated with that transfer.

  3. 14 CFR 25.1431 - Electronic equipment.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... equipment and their installations, critical environmental conditions must be considered. (b) Radio and... electronic equipment, controls, and wiring must be installed so that operation of any one unit or system of...

  4. Reversed Phase Column HPLC-ICP-MS Conditions for Arsenic Speciation Analysis of Rice Flour.

    PubMed

    Narukawa, Tomohiro; Matsumoto, Eri; Nishimura, Tsutomu; Hioki, Akiharu

    2015-01-01

    New measurement conditions for arsenic speciation analysis of rice flour were developed using HPLC-ICP-MS equipped with a reversed phase ODS column. Eight arsenic species, namely, arsenite [As(III)], arsenate [As(V)], monomethylarsonic acid (MMAA), dimethylarsinic acid (DMAA), trimethylarsine oxide (TMAO), tetramethylarsonium (TeMA), arsenobetaine (AsB) and arsenocholine (AsC), were separated and determined under the proposed conditions. In particular, As(III) and MMAA and DMAA and AsB were completely separated using a newly proposed eluent containing ammonium dihydrogen phosphate. Importantly, the sensitivity changes, in particular those of As(V) and As(III) caused by coexisting elements and by complex matrix composition, which had been problematical in previously reported methods, were eliminated. The new eluent can be applied to C8, C18 and C30 ODS columns with the same effectiveness and with excellent repeatability. The proposed analytical method was successfully applied to extracts of rice flour certified reference materials.

  5. Data communication between data terminal equipment and the JPL administrative data base management system

    NASA Technical Reports Server (NTRS)

    Iverson, R. W.

    1984-01-01

    Approaches to enabling an installed base of mixed data terminal equipment to access a data base management system designed to work with a specific terminal are discussed. The approach taken by the Jet Propulsion Laboratory is described. Background information on the Jet Propulsion Laboratory (JPL), its organization and a description of the Administrative Data Base Management System is included.

  6. Complementary equipment for controlling multiple laser beams on single scanner MPLSM systems

    NASA Astrophysics Data System (ADS)

    Helm, P. Johannes; Nase, Gabriele; Heggelund, Paul; Reppen, Trond

    2010-02-01

    Multi-Photon-Laser-Scanning-Microscopy (MPLSM) now stands as one of the most powerful experimental tools in biology. Specifically, MPLSM based in-vivo studies of structures and processes in the brains of small rodents and imaging in brain-slices have led to considerable progress in the field of neuroscience. Equipment allowing for independent control of two laser-beams, one for imaging and one for photochemical manipulation, strongly enhances any MPLSM platform. Some industrial MPLSM producers have introduced double scanner options in MPLSM systems. Here, we describe the upgrade of a single scanner MPLSM system with equipment that is suitable for independently controlling the beams of two Titanium Sapphire lasers. The upgrade is compatible with any actual MPLSM system and can be combined with any commercial or self assembled system. Making use of the pixel-clock, frame-active and line-active signals provided by the scanner-electronics of the MPLSM, the user can, by means of an external unit, select individual pixels or rectangular ROIs within the field of view of an overview-scan to be exposed, or not exposed, to the beam(s) of one or two lasers during subsequent scans. The switching processes of the laser-beams during the subsequent scans are performed by means of Electro-Optical-Modulators (EOMs). While this system does not provide the flexibility of two-scanner modules, it strongly enhances the experimental possibilities of one-scanner systems provided a second laser and two independent EOMs are available. Even multi-scanner-systems can profit from this development, which can be used to independently control any number of laser beams.

  7. Strategy Guideline: HVAC Equipment Sizing

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Burdick, A.

    The heating, ventilation, and air conditioning (HVAC) system is arguably the most complex system installed in a house and is a substantial component of the total house energy use. A right-sized HVAC system will provide the desired occupant comfort and will run efficiently. This Strategy Guideline discusses the information needed to initially select the equipment for a properly designed HVAC system. Right-sizing of an HVAC system involves the selection of equipment and the design of the air distribution system to meet the accurate predicted heating and cooling loads of the house. Right-sizing the HVAC system begins with an accurate understandingmore » of the heating and cooling loads on a space; however, a full HVAC design involves more than just the load estimate calculation - the load calculation is the first step of the iterative HVAC design procedure. This guide describes the equipment selection of a split system air conditioner and furnace for an example house in Chicago, IL as well as a heat pump system for an example house in Orlando, Florida. The required heating and cooling load information for the two example houses was developed in the Department of Energy Building America Strategy Guideline: Accurate Heating and Cooling Load Calculations.« less

  8. 46 CFR 77.40-1 - Pilot boarding equipment.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 46 Shipping 3 2010-10-01 2010-10-01 false Pilot boarding equipment. 77.40-1 Section 77.40-1... MISCELLANEOUS SYSTEMS AND EQUIPMENT Pilot Boarding Equipment § 77.40-1 Pilot boarding equipment. (a) This... vessel. (b) Each vessel must have suitable pilot boarding equipment available for use on each side of the...

  9. HPLC and HPLC/MS/MS Studies on Stress, Accelerated and Intermediate Degradation Tests of Antivirally Active Tricyclic Analog of Acyclovir.

    PubMed

    Lesniewska, Monika A; Dereziński, Paweł; Klupczyńska, Agnieszka; Kokot, Zenon J; Ostrowski, Tomasz; Zeidler, Joanna; Muszalska, Izabela

    2015-01-01

    The degradation behavior of a tricyclic analog of acyclovir [6-(4-MeOPh)-TACV] was determined in accordance with International Conference on Harmonization guidelines for good clinical practice under different stress conditions (neutral hydrolysis, strong acid/base degradation, oxidative decomposition, photodegradation, and thermal degradation). Accelerated [40±2°C/75%±5% relative humidity (RH)] and intermediate (30±2°C/65%±5% RH) stability tests were also performed. For observation of the degradation of the tested compound the RP-HPLC was used, whereas for the analysis of its degradation products HPLC/MS/MS was used. Degradation of the tested substance allowed its classification as unstable in neutral environment, acidic/alkaline medium, and in the presence of oxidizing agent. The tested compound was also light sensitive and was classified as photolabile both in solution and in the solid phase. However, the observed photodegradation in the solid phase was at a much lower level than in the case of photodegradation in solution. The study showed that both air temperature and RH had no significant effect on the stability of the tested substance during storage for 1 month at 100°C (dry heat) as well as during accelerated and intermediate tests. Based on the HPLC/MS/MS analysis, it can be concluded that acyclovir was formed as a degradation product of 6-(4-MeOPh)-TACV.

  10. 48 CFR 870.115 - Food service equipment.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 48 Federal Acquisition Regulations System 5 2011-10-01 2011-10-01 false Food service equipment... DEPARTMENT SUPPLEMENTARY REGULATIONS SPECIAL PROCUREMENT CONTROLS Controls 870.115 Food service equipment. (a) All new food service equipment purchased for Dietetic Service through other than Defense General...

  11. 48 CFR 870.115 - Food service equipment.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 48 Federal Acquisition Regulations System 5 2012-10-01 2012-10-01 false Food service equipment... DEPARTMENT SUPPLEMENTARY REGULATIONS SPECIAL PROCUREMENT CONTROLS Controls 870.115 Food service equipment. (a) All new food service equipment purchased for Dietetic Service through other than Defense General...

  12. 48 CFR 870.115 - Food service equipment.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 48 Federal Acquisition Regulations System 5 2014-10-01 2014-10-01 false Food service equipment... DEPARTMENT SUPPLEMENTARY REGULATIONS SPECIAL PROCUREMENT CONTROLS Controls 870.115 Food service equipment. (a) All new food service equipment purchased for Dietetic Service through other than Defense General...

  13. 48 CFR 870.115 - Food service equipment.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 48 Federal Acquisition Regulations System 5 2013-10-01 2013-10-01 false Food service equipment... DEPARTMENT SUPPLEMENTARY REGULATIONS SPECIAL PROCUREMENT CONTROLS Controls 870.115 Food service equipment. (a) All new food service equipment purchased for Dietetic Service through other than Defense General...

  14. 48 CFR 870.115 - Food service equipment.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 48 Federal Acquisition Regulations System 5 2010-10-01 2010-10-01 false Food service equipment... DEPARTMENT SUPPLEMENTARY REGULATIONS SPECIAL PROCUREMENT CONTROLS Controls 870.115 Food service equipment. (a) All new food service equipment purchased for Dietetic Service through other than Defense General...

  15. Analysis of limette and bergamot distilled essential oils by HPLC.

    PubMed

    Buiarelli, Francesca; Cartoni, Giampaolo; Coccioli, Franco; Jasionowska, Renata; Mazzarino, Monica

    2002-04-01

    This work examines the distilled essential oils of limette and bergamot in order to assess the presence of low volatile substances such as coumarins (bergapten) which, being toxic, must be eliminated before using these oils in the food industry. The quantitative determination of coumarins was carried out by spectrofluorimetric detection. The substances present in the chromatograms, obtained by HPLC with UV detection at 254 nm, were then identified. Moreover, a new coumarin that is present in small quantities was identified using HPLC-MS.

  16. 46 CFR 111.05-33 - Equipment safety grounding (bonding) conductors.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 46 Shipping 4 2012-10-01 2012-10-01 false Equipment safety grounding (bonding) conductors. 111.05... ENGINEERING ELECTRIC SYSTEMS-GENERAL REQUIREMENTS Equipment Ground, Ground Detection, and Grounded Systems § 111.05-33 Equipment safety grounding (bonding) conductors. (a) Each equipment-grounding conductor must...

  17. 46 CFR 111.05-33 - Equipment safety grounding (bonding) conductors.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 46 Shipping 4 2014-10-01 2014-10-01 false Equipment safety grounding (bonding) conductors. 111.05... ENGINEERING ELECTRIC SYSTEMS-GENERAL REQUIREMENTS Equipment Ground, Ground Detection, and Grounded Systems § 111.05-33 Equipment safety grounding (bonding) conductors. (a) Each equipment-grounding conductor must...

  18. 46 CFR 111.05-33 - Equipment safety grounding (bonding) conductors.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 46 Shipping 4 2013-10-01 2013-10-01 false Equipment safety grounding (bonding) conductors. 111.05... ENGINEERING ELECTRIC SYSTEMS-GENERAL REQUIREMENTS Equipment Ground, Ground Detection, and Grounded Systems § 111.05-33 Equipment safety grounding (bonding) conductors. (a) Each equipment-grounding conductor must...

  19. Environmentally evaluated HPLC-ELSD method to monitor enzymatic synthesis of a non-ionic surfactant.

    PubMed

    Gaber, Yasser; Akerman, Cecilia Orellana; Hatti-Kaul, Rajni

    2014-01-01

    N-Lauroyl-N-methylglucamide is a biodegradable surfactant derived from renewable resources. In an earlier study, we presented an enzymatic solvent-free method for synthesis of this compound. In the present report, the HPLC method developed to follow the reaction between lauric acid/methyl laurate and N-methyl glucamine (MEG) and its environmental assessment are described. Use of ultraviolet (UV) absorption or refractive index (RI) detectors did not allow the detection of N-methyl glucamine (MEG). With Evaporative light scattering detector ELSD, it was possible to apply a gradient elution, and detect MEG with a limit of detection, LOD = 0.12 μg. A good separation of the peaks: MEG, lauric acid, product (amide) and by-product (amide-ester) was achieved with the gradient program with a run time of 40 min. The setting of ELSD detector was optimized using methyl laurate as the analyte. LC-MS/MS was used to confirm the amide and amide-ester peaks. We evaluated the greenness of the developed method using the freely available software HPLC-Environmental Assessment Tool (HPLC-EAT) and the method got a scoring of 73 HPLC-EAT units, implying that the analytical procedure was more environmentally benign compared to some other methods reported in literature whose HPLC-EAT values scored up to 182. Use of ELSD detector allowed the detection and quantification of the substrates and the reaction products of enzymatic synthesis of the surfactant, N-lauroyl-N-methylglucamide. The developed HPLC method has acceptable environmental profile based on HPLC-EAT evaluation.

  20. Environmentally evaluated HPLC-ELSD method to monitor enzymatic synthesis of a non-ionic surfactant

    PubMed Central

    2014-01-01

    Background N-Lauroyl-N-methylglucamide is a biodegradable surfactant derived from renewable resources. In an earlier study, we presented an enzymatic solvent-free method for synthesis of this compound. In the present report, the HPLC method developed to follow the reaction between lauric acid/methyl laurate and N-methyl glucamine (MEG) and its environmental assessment are described. Results Use of ultraviolet (UV) absorption or refractive index (RI) detectors did not allow the detection of N-methyl glucamine (MEG). With Evaporative light scattering detector ELSD, it was possible to apply a gradient elution, and detect MEG with a limit of detection, LOD = 0.12 μg. A good separation of the peaks: MEG, lauric acid, product (amide) and by-product (amide-ester) was achieved with the gradient program with a run time of 40 min. The setting of ELSD detector was optimized using methyl laurate as the analyte. LC-MS/MS was used to confirm the amide and amide-ester peaks. We evaluated the greenness of the developed method using the freely available software HPLC-Environmental Assessment Tool (HPLC-EAT) and the method got a scoring of 73 HPLC-EAT units, implying that the analytical procedure was more environmentally benign compared to some other methods reported in literature whose HPLC-EAT values scored up to 182. Conclusion Use of ELSD detector allowed the detection and quantification of the substrates and the reaction products of enzymatic synthesis of the surfactant, N-lauroyl-N-methylglucamide. The developed HPLC method has acceptable environmental profile based on HPLC-EAT evaluation. PMID:24914404

  1. Quantification of appetite suppressing steroid glycosides from Hoodia gordonii in dried plant material, purified extracts and food products using HPLC-UV and HPLC-MS methods.

    PubMed

    Janssen, Hans-Gerd; Swindells, Chris; Gunning, Philip; Wang, Weijun; Grün, Christian; Mahabir, Krishna; Maharaj, Vinesh J; Apps, Peter J

    2008-06-09

    High-performance liquid chromatography (HPLC)-UV and HPLC-Mass Spectrometry (MS) methods were developed for the quantitative analysis of the family of Hoodia gordonii steroid glycosides with appetite suppressing properties in dried plant material, in purified and enriched extracts and in various prototype food-products fortified with H. gordonii extracts. For solid materials, e.g. dried plants or for non-fatty foods, extraction of the steroid glycosides is performed using methanol. For products where the steroid glycosides are present in an oil matrix, direct injection of the oil after dilution in tetrahydrofuran is applied. The HPLC separation is performed on an octyl-modified reversed-phase column in the gradient mode with UV detection at lambda = 220 nm. Quantification is performed against an external calibration line prepared using either one of the pure steroid glycosides or geranyl-tiglate. Short- and long-term repeatabilities of the methods are better than 3 and 6%, respectively. Recoveries are better than 85%, even in the analysis of the least abundant steroid glycosides in a complex yoghurt drink. Linearity is better than 3-4 orders of magnitude and the detection limits are below approximately 2 microg g(-1) for the individual steroid glycosides in dried plant material and food products. HPLC-MS is used to confirm that the steroid glycosides contain the characteristic steroid core, the carbohydrate chain and the tigloyl group.

  2. 40 CFR 82.36 - Approved refrigerant handling equipment.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... Approved refrigerant handling equipment. (a)(1) Refrigerant recycling equipment must be certified by the...) Recovery/Recycling Equipment and Recovery/Recycling/Recharging for Mobile Air-Conditioning Systems. (5... Recycling Equipment Intended for Use with both CFC-12 and HFC-134a, Recommended Service Procedure for the...

  3. 40 CFR 82.36 - Approved refrigerant handling equipment.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... Approved refrigerant handling equipment. (a)(1) Refrigerant recycling equipment must be certified by the...) Recovery/Recycling Equipment and Recovery/Recycling/Recharging for Mobile Air-Conditioning Systems. (5... Recycling Equipment Intended for Use with both CFC-12 and HFC-134a, Recommended Service Procedure for the...

  4. 40 CFR 82.36 - Approved refrigerant handling equipment.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... Approved refrigerant handling equipment. (a)(1) Refrigerant recycling equipment must be certified by the...) Recovery/Recycling Equipment and Recovery/Recycling/Recharging for Mobile Air-Conditioning Systems. (5... Recycling Equipment Intended for Use with both CFC-12 and HFC-134a, Recommended Service Procedure for the...

  5. 40 CFR 82.36 - Approved refrigerant handling equipment.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... Approved refrigerant handling equipment. (a)(1) Refrigerant recycling equipment must be certified by the...) Recovery/Recycling Equipment and Recovery/Recycling/Recharging for Mobile Air-Conditioning Systems. (5... Recycling Equipment Intended for Use with both CFC-12 and HFC-134a, Recommended Service Procedure for the...

  6. 40 CFR 82.36 - Approved refrigerant handling equipment.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... Approved refrigerant handling equipment. (a)(1) Refrigerant recycling equipment must be certified by the...) Recovery/Recycling Equipment and Recovery/Recycling/Recharging for Mobile Air-Conditioning Systems. (5... Recycling Equipment Intended for Use with both CFC-12 and HFC-134a, Recommended Service Procedure for the...

  7. 14 CFR 121.337 - Protective breathing equipment.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... REQUIREMENTS: DOMESTIC, FLAG, AND SUPPLEMENTAL OPERATIONS Instrument and Equipment Requirements § 121.337... systems other than chemical oxygen generators, there must be a means to allow the crew to readily... fully charged. (iv) For each chemical oxygen generator, the supply system equipment must meet the...

  8. Development of an HPLC method for determination of metabolic compounds in myocardial tissue.

    PubMed

    Volonté, M G; Yuln, G; Quiroga, P; Consolini, A E

    2004-05-28

    The determination of adenine nucleotides and creatine compounds has great importance in the characterization of ischemic myocardial injury and post-ischemic recovery. It was developed by an HPLC method for the quantification of creatine (Cr), creatine phosphate (CrP), hypoxanthine (HX), AMP, adenosine (Ad), ADP and ATP in isolated perfused rat hearts. The chromatographic conditions were: RP 18 column; mobile phase composed by KH(2)PO(4) (215 mM), tetrabutylammonium hydrogen sulfate (2.3mM), acetonitrile (4%) and KOH (1M 0.4%); flow rate 1 ml min(-1); temperature 25 degrees C; injection volume 20 microl; detection at 220 nm and height peak (HP) as the integration parameter. The method was validated by means of linearity and sensitivity evaluations, using calibration curves done with five concentration levels of each compound. The limits of quantification (LOQ) were also determined. The system precision was calculated as the coefficient of variation for five injections for each compound tested. The purity of the peaks was established using enzymatic peak shift analysis with hexokinase and creatine kinase and also comparing HP at various wavelengths. Frozen hearts were homogenized with a mechanical homogenizer for 3 min at 0 degrees C added with 5 ml of 0.4N HCLO(4). After precipitation with 0.8 ml of 2M KOH the extract was shaked for 2 min and later centrifuged at 0 degrees C for 10 min. The supernatant was kept on ice, filtrated and injected into the HPLC system. The results show that the method for the determination of Cr, CrP, HX, AMP, Ad, ADP and ATP by HPLC here described has good linearity, LOQ, precision, specificity and is simple and rapid to perform.

  9. 18 CFR 367.3980 - Account 398, Miscellaneous equipment.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... service company's operations that are not included in any other account of this system of accounts. (b... equipment. (3) Employees' recreation equipment. (4) Radios. (5) Restaurant equipment. (6) Soda fountains. (7...

  10. 48 CFR 52.246-19 - Warranty of Systems and Equipment under Performance Specifications or Design Criteria.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 48 Federal Acquisition Regulations System 2 2014-10-01 2014-10-01 false Warranty of Systems and Equipment under Performance Specifications or Design Criteria. 52.246-19 Section 52.246-19 Federal Acquisition Regulations System FEDERAL ACQUISITION REGULATION (CONTINUED) CLAUSES AND FORMS SOLICITATION PROVISIONS AND CONTRACT CLAUSES Text of...

  11. Cyber and physical equipment digital control system in Industry 4.0 item designing company

    NASA Astrophysics Data System (ADS)

    Gurjanov, A. V.; Zakoldaev, D. A.; Shukalov, A. V.; Zharinov, I. O.

    2018-05-01

    The problem of organization of digital control of the item designing company equipped with cyber and physical systems is being studied. A scheme of cyber and physical systems and personnel interaction in the Industry 4.0 smart factory company is presented. A scheme of assembly units transportation in the Industry 4.0 smart factory company is provided. A scheme of digital control system in the Industry 4.0 smart factory company is given.

  12. Anesthesia equipment malfunction: origins and clinical recognition.

    PubMed Central

    McIntyre, J. W.

    1979-01-01

    Equipment malfunction is a problem of particular importance during anesthesia and resuscitation. A review of published reports shows that the most common clinical events involve endotracheal tubes, the inspired oxygen concentration, the volume of inspired anesthetic vapours and gases, and pressures in the breathing or ventilation system. It is concluded that protection of a patient from equipment malfunction depends on: (a) appropriate application of standards set by a national standards association; (b) careful evaluation of equipment prior to purchase; (c) comprehension of equipment function by the user; (d) conscientious routine servicing of all systems concerned with anesthesia and resuscitation, and checking after service and before clinical use; (e) preanesthesia testing of equipment, including the use of an oxygen analyser in the breathing circuit; (f) early inclusion of equipment malfunction in the differential diagnosis of events during anesthesia; and (g) rapid action that cannot present a new hazard to the patient to correct the results of apparatus malfunction. PMID:436069

  13. 14 CFR 121.305 - Flight and navigational equipment.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 14 Aeronautics and Space 3 2013-01-01 2013-01-01 false Flight and navigational equipment. 121.305... Flight and navigational equipment. No person may operate an airplane unless it is equipped with the following flight and navigational instruments and equipment: (a) An airspeed indicating system with heated...

  14. 14 CFR 121.305 - Flight and navigational equipment.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 14 Aeronautics and Space 3 2011-01-01 2011-01-01 false Flight and navigational equipment. 121.305... Flight and navigational equipment. No person may operate an airplane unless it is equipped with the following flight and navigational instruments and equipment: (a) An airspeed indicating system with heated...

  15. 14 CFR 121.305 - Flight and navigational equipment.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 14 Aeronautics and Space 3 2012-01-01 2012-01-01 false Flight and navigational equipment. 121.305... Flight and navigational equipment. No person may operate an airplane unless it is equipped with the following flight and navigational instruments and equipment: (a) An airspeed indicating system with heated...

  16. 14 CFR 121.305 - Flight and navigational equipment.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 14 Aeronautics and Space 3 2014-01-01 2014-01-01 false Flight and navigational equipment. 121.305... Flight and navigational equipment. No person may operate an airplane unless it is equipped with the following flight and navigational instruments and equipment: (a) An airspeed indicating system with heated...

  17. Identification and anti-oxidant capacity determination of phenolics and their glycosides in elderflower by on-line HPLC-CUPRAC method.

    PubMed

    Çelik, S Esin; Özyürek, Mustafa; Güçlü, Kubilay; Çapanoğlu, Esra; Apak, Reşat

    2014-01-01

    Development and application of an on-line cupric reducing anti-oxidant capacity (CUPRAC) assay coupled with HPLC for separation and on-line determination of phenolic anti-oxidants in elderflower (Sambucus nigra L.) extracts for their anti-oxidant capacity are significant for evaluating health-beneficial effects. Moreover, this work aimed to assay certain flavonoid glycosides of elderflower that could not be identified/quantified by other similar on-line HPLC methods (i.e. 2,2-diphenyl-1-picrylhdrazyl and 2, 2'-azino-bis-3-ethylbenzothiazoline-6-sulphonic acid). To identify anti-oxidant constituents in elderflower by HPLC and to evaluate their individual anti-oxidant capacities by on-line HPLC-CUPRAC assay with a post-column derivatisation system. The separation and UV detection of polyphenols were performed on a C18 -column using gradient elution with two different mobile phase solutions, that is acetonitrile and 1% glacial acetic acid, with detection at 340 nm. The HPLC-separated anti-oxidant polyphenols in column effluent react with copper(II)-neocuproine in a reaction-coil to reduce the latter to copper(I)-neocuproine (Cu(I)-Nc) chelate having maximum absorption at 450 nm. The detection limits of tested compounds at 450 nm after post-column derivatisation were compared with those of at 340 nm UV-detection without derivatisation. LOD values (µg/mL) of quercetin and its glycosides at 450 nm were lower than those of UV detection at 340 nm. This method was applied successfully to elderflower extract. The flavonol glycosides of quercetin and kaempferol bound to several sugar components (glucose, rhamnose, galactose and rutinose) were identified in the sample. The on-line HPLC-CUPRAC method was advantageous over on-line ABTS and DPPH methods for measuring the flavonoid glycosides of elderflower. Copyright © 2014 John Wiley & Sons, Ltd.

  18. Measurement of menadione in urine by HPLC

    USDA-ARS?s Scientific Manuscript database

    Mammals convert phylloquinone to MK-4, with menadione as a possible intermediate. We developed and validated a method measuring urinary menadione. A high performance liquid chromatography (HPLC) method with a C30 column, fluorescence detection and post-column zinc reduction was developed. The mobile...

  19. Electronic circuits and systems: A compilation. [including integrated circuits, logic circuits, varactor diode circuits, low pass filters, and optical equipment circuits

    NASA Technical Reports Server (NTRS)

    1975-01-01

    Technological information is presented electronic circuits and systems which have potential utility outside the aerospace community. Topics discussed include circuit components such as filters, converters, and integrators, circuits designed for use with specific equipment or systems, and circuits designed primarily for use with optical equipment or displays.

  20. 47 CFR 74.451 - Certification of equipment.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 47 Telecommunication 4 2010-10-01 2010-10-01 false Certification of equipment. 74.451 Section 74... Broadcast Stations § 74.451 Certification of equipment. (a) Applications for new remote pickup broadcast stations or systems or for changing transmitting equipment of an existing station will not be accepted...

  1. Quantification of astaxanthin in shrimp waste hydrolysate by HPLC.

    PubMed

    López-Cervantes, J; Sánchez-Machado, D I; Gutiérrez-Coronado, M A; Ríos-Vázquez, N J

    2006-10-01

    In the present study, a simple and rapid reversed-phase HPLC method for the determination of astaxanthin in shrimp waste hydrolysate has been developed and validated. The analytical procedure involves the direct extraction of astaxanthin from the lipid fraction with methanol. The analytical column, SS Exil ODS, was operated at 25C. The mobile phase consisted of a mixture of water:methanol:dichloromethane:acetonitrile (4.5:28:22:45.5 v/v/v/v) at a flow rate of 1.0 mL/min. Detection and identification were performed using a photodiode array detector (lambda(detection) = 476 nm). The proposed HPLC method showed adequate linearity, repeatability and accuracy.

  2. Prepositioned Stocks: Marine Corps Needs to Improve Cost Estimate Reliability and Oversight of Inventory Systems for Equipment in Norway

    DTIC Science & Technology

    2015-09-01

    HMMWV), M1A1 Main Battle Tanks, Tank Retrievers, Armored Breeching Vehicles, Amphibious Assault Vehicles, and several variants of the Medium...MCPP-N equipment stored in the Norwegian caves. As noted earlier, Marine Corps equipment is distributed among six caves. While the current version of...according to Marine Corps Business System Integration Team officials, the initial plan was for the first version of the Global Combat Support System

  3. Sub-cooled liquid nitrogen cryogenic system with neon turbo-refrigerator for HTS power equipment

    NASA Astrophysics Data System (ADS)

    Yoshida, S.; Hirai, H.; Nara, N.; Ozaki, S.; Hirokawa, M.; Eguchi, T.; Hayashi, H.; Iwakuma, M.; Shiohara, Y.

    2014-01-01

    We developed a prototype sub-cooled liquid nitrogen (LN) circulation system for HTS power equipment. The system consists of a neon turbo-Brayton refrigerator with a LN sub-cooler and LN circulation pump unit. The neon refrigerator has more than 2 kW cooling power at 65 K. The LN sub-cooler is a plate-fin type heat exchanger and is installed in a refrigerator cold box. In order to carry out the system performance tests, a dummy cryostat having an electric heater was set instead of a HTS power equipment. Sub-cooled LN is delivered into the sub-cooler by the LN circulation pump and cooled within it. After the sub-cooler, sub-cooled LN goes out from the cold box to the dummy cryostat, and comes back to the pump unit. The system can control an outlet sub-cooled LN temperature by adjusting refrigerator cooling power. The refrigerator cooling power is automatically controlled by the turbo-compressor rotational speed. In the performance tests, we increased an electric heater power from 200 W to 1300 W abruptly. We confirmed the temperature fluctuation was about ±1 K. We show the cryogenic system details and performance test results in this paper.

  4. Comparison of the BioRad Variant and Primus Ultra2 high-pressure liquid chromatography (HPLC) instruments for the detection of variant hemoglobins.

    PubMed

    Gosselin, R C; Carlin, A C; Dwyre, D M

    2011-04-01

    Hemoglobin variants are a result of genetic changes resulting in abnormal or dys-synchronous hemoglobin chain production (thalassemia) or the generation of hemoglobin chain variants such as hemoglobin S. Automated high-pressure liquid chromatography (HPLC) systems have become the method of choice for the evaluation of patients suspected with hemoglobinopathies. In this study, we evaluated the performance of two HPLC methods used in the detection of common hemoglobin variants: Variant and Ultra2. There were 377 samples tested, 26% (99/377) with HbS, 8.5% (32/377) with HbC, 20.7% (78/377) with other hemoglobin variant or thalassemia, and 2.9% with increased hemoglobin A(1) c. The interpretations of each chromatograph were compared. There were no differences noted for hemoglobins A(0), S, or C. There were significant differences between HPLC methods for hemoglobins F, A(2), and A(1) c. However, there was good concordance between normal and abnormal interpretations (97.9% and 96.2%, respectively). Both Variant and Ultra2 HPLC methods were able to detect most common hemoglobin variants. There was better discrimination for fast hemoglobins, between hemoglobins E and A(2), and between hemoglobins S and F using the Ultra2 HPLC method. © 2010 Blackwell Publishing Ltd.

  5. Binding patterns of vanadium ions with different valence states to human serum transferrin studied by HPLC/high-resolution ICP-MS.

    PubMed

    Nagaoka, Megumi Hamano; Yamazaki, Takeshi; Maitani, Tamio

    2002-09-06

    Vanadium (V) is an essential metal for mammals and has different valence states. In blood, V is bound to serum transferrin (Tf), a glycoprotein which has two metal-binding sites, and carbonate is generally required for the binding. In this study, the binding patterns of V(III), V(IV), and V(V) to human serum Tf (hTf) were analyzed using an HPLC system equipped with an anion-exchange column and directly connected to a high-resolution inductively coupled plasma-mass spectrometer for metal detection (51V). In affinity to hTf, the three ions were ranked V(III)>V(IV)>V(V) in the presence of bicarbonate and V(III) reverse congruent V(IV)>V(V) in the absence. Intermediates in the "open forms" binding to the respective sites were detected at the initial stage. V(IV) and V(V) were bound to the N-lobe site in the "closed form" and "open form," respectively. In the absence of bicarbonate, V ions with respective valence states were bound to hTf in the "open form." In terms of binding to hTf, tri-valent V was most favorable in the presence of bicarbonate.

  6. 46 CFR 96.40-1 - Pilot boarding equipment.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 46 Shipping 4 2011-10-01 2011-10-01 false Pilot boarding equipment. 96.40-1 Section 96.40-1... CONTROL AND MISCELLANEOUS SYSTEMS AND EQUIPMENT Pilot Boarding Equipment § 96.40-1 Pilot boarding equipment. (a) This section applies to each vessel that normally embarks or disembarks a pilot from a pilot...

  7. 46 CFR 195.40-1 - Pilot boarding equipment.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 46 Shipping 7 2010-10-01 2010-10-01 false Pilot boarding equipment. 195.40-1 Section 195.40-1... CONTROL AND MISCELLANEOUS SYSTEMS AND EQUIPMENT Pilot Boarding Equipment § 195.40-1 Pilot boarding equipment. (a) This section applies to each vessel that normally embarks or disembarks a pilot from a pilot...

  8. 46 CFR 96.40-1 - Pilot boarding equipment.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 46 Shipping 4 2010-10-01 2010-10-01 false Pilot boarding equipment. 96.40-1 Section 96.40-1... CONTROL AND MISCELLANEOUS SYSTEMS AND EQUIPMENT Pilot Boarding Equipment § 96.40-1 Pilot boarding equipment. (a) This section applies to each vessel that normally embarks or disembarks a pilot from a pilot...

  9. 46 CFR 195.40-1 - Pilot boarding equipment.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 46 Shipping 7 2011-10-01 2011-10-01 false Pilot boarding equipment. 195.40-1 Section 195.40-1... CONTROL AND MISCELLANEOUS SYSTEMS AND EQUIPMENT Pilot Boarding Equipment § 195.40-1 Pilot boarding equipment. (a) This section applies to each vessel that normally embarks or disembarks a pilot from a pilot...

  10. 14 CFR 25.1441 - Oxygen equipment and supply.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 14 Aeronautics and Space 1 2011-01-01 2011-01-01 false Oxygen equipment and supply. 25.1441... Oxygen equipment and supply. (a) If certification with supplemental oxygen equipment is requested, the equipment must meet the requirements of this section and §§ 25.1443 through 25.1453. (b) The oxygen system...

  11. 14 CFR 25.1441 - Oxygen equipment and supply.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 14 Aeronautics and Space 1 2010-01-01 2010-01-01 false Oxygen equipment and supply. 25.1441... Oxygen equipment and supply. (a) If certification with supplemental oxygen equipment is requested, the equipment must meet the requirements of this section and §§ 25.1443 through 25.1453. (b) The oxygen system...

  12. 14 CFR 25.1441 - Oxygen equipment and supply.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 14 Aeronautics and Space 1 2014-01-01 2014-01-01 false Oxygen equipment and supply. 25.1441... Oxygen equipment and supply. (a) If certification with supplemental oxygen equipment is requested, the equipment must meet the requirements of this section and §§ 25.1443 through 25.1453. (b) The oxygen system...

  13. 14 CFR 25.1441 - Oxygen equipment and supply.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 14 Aeronautics and Space 1 2012-01-01 2012-01-01 false Oxygen equipment and supply. 25.1441... Oxygen equipment and supply. (a) If certification with supplemental oxygen equipment is requested, the equipment must meet the requirements of this section and §§ 25.1443 through 25.1453. (b) The oxygen system...

  14. 14 CFR 25.1441 - Oxygen equipment and supply.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 14 Aeronautics and Space 1 2013-01-01 2013-01-01 false Oxygen equipment and supply. 25.1441... Oxygen equipment and supply. (a) If certification with supplemental oxygen equipment is requested, the equipment must meet the requirements of this section and §§ 25.1443 through 25.1453. (b) The oxygen system...

  15. 47 CFR 80.1103 - Equipment authorization.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 47 Telecommunication 5 2011-10-01 2011-10-01 false Equipment authorization. 80.1103 Section 80.1103 Telecommunication FEDERAL COMMUNICATIONS COMMISSION (CONTINUED) SAFETY AND SPECIAL RADIO SERVICES STATIONS IN THE MARITIME SERVICES Global Maritime Distress and Safety System (GMDSS) Equipment Requirements...

  16. 47 CFR 80.1103 - Equipment authorization.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 47 Telecommunication 5 2014-10-01 2014-10-01 false Equipment authorization. 80.1103 Section 80.1103 Telecommunication FEDERAL COMMUNICATIONS COMMISSION (CONTINUED) SAFETY AND SPECIAL RADIO SERVICES STATIONS IN THE MARITIME SERVICES Global Maritime Distress and Safety System (GMDSS) Equipment Requirements...

  17. 47 CFR 80.1103 - Equipment authorization.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 47 Telecommunication 5 2012-10-01 2012-10-01 false Equipment authorization. 80.1103 Section 80.1103 Telecommunication FEDERAL COMMUNICATIONS COMMISSION (CONTINUED) SAFETY AND SPECIAL RADIO SERVICES STATIONS IN THE MARITIME SERVICES Global Maritime Distress and Safety System (GMDSS) Equipment Requirements...

  18. 47 CFR 80.1103 - Equipment authorization.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 47 Telecommunication 5 2013-10-01 2013-10-01 false Equipment authorization. 80.1103 Section 80.1103 Telecommunication FEDERAL COMMUNICATIONS COMMISSION (CONTINUED) SAFETY AND SPECIAL RADIO SERVICES STATIONS IN THE MARITIME SERVICES Global Maritime Distress and Safety System (GMDSS) Equipment Requirements...

  19. 46 CFR 169.705 - Mooring equipment.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) NAUTICAL SCHOOLS SAILING SCHOOL VESSELS Vessel Control, Miscellaneous Systems, and Equipment § 169.705 Mooring equipment. Each vessel must be fitted with... the size of the vessel and the waters on which it operates. ...

  20. Measurement of Menadione in Urine by HPLC

    PubMed Central

    Rajabi, Ala Al; Peterson, James; Choi, Sang Woon; Suttie, John; Barakat, Susan; Booth, Sarah L

    2010-01-01

    Menadione is a metabolite of vitamin K that is excreted in urine. A high performance liquid chromatography (HPLC) method using a C30 column, post-column zinc reduction and fluorescence detection was developed to measure urinary menadione. The mobile phase was composed of 95% methanol with 0.55% aqueous solution and 5% DI H2O. Menaquinone-2 (MK-2) was used as an internal standard. The standard calibration curve was linear with a correlation coefficient (R2) of 0.999 for both menadione and MK-2. The lower limit of quantification (LLOQ) was 0.3 pmole menadione/mL urine. Sample preparation involved hydrolysis of menadiol conjugates and oxidizing the released menadiol to menadione. Using this method, urinary menadione was shown to increase in response to 3 years of phylloquinone supplementation. This HPLC method is a sensitive and reproducible way to detect menadione in urine. Research support: USDA ARS Cooperative Agreement 58-1950-7-707. PMID:20719580

  1. Measurement of menadione in urine by HPLC.

    PubMed

    Al Rajabi, Ala; Peterson, James; Choi, Sang-Woon; Suttie, John; Barakat, Susan; Booth, Sarah L

    2010-09-15

    Menadione is a metabolite of vitamin K that is excreted in urine. A high performance liquid chromatography (HPLC) method using a C(30) column, post-column zinc reduction and fluorescence detection was developed to measure urinary menadione. The mobile phase was composed of 95% methanol with 0.55% aqueous solution and 5% DI H(2)O. Menaquinone-2 (MK-2) was used as an internal standard. The standard calibration curve was linear with a correlation coefficient (R(2)) of 0.999 for both menadione and MK-2. The lower limit of quantification (LLOQ) was 0.3pmole menadione/mL urine. Sample preparation involved hydrolysis of menadiol conjugates and oxidizing the released menadiol to menadione. Using this method, urinary menadione was shown to increase in response to 3 years of phylloquinone supplementation. This HPLC method is a sensitive and reproducible way to detect menadione in urine. Copyright (c) 2010 Elsevier B.V. All rights reserved.

  2. Determination of Two Sulfonylurea Herbicides Residues in Soil Environment Using HPLC and Phytotoxicity of These Herbicides by Lentil Bioassay.

    PubMed

    Mehdizadeh, Mohammad; Alebrahim, Mohammad Taghi; Roushani, Mahmoud

    2017-07-01

    A HPLC-UV detection system was used for determination of sulfosulfuron and tribenuron methyl residues from soils. The soils were fortified with sulfosulfuron and tribenuron methyl at rates of 26 and 15 g a.i. ha -1 respectively and samples were taken randomly on 0 (2 h), 1, 2, 4, 10, 20, 40, 60, 90 and 120 days after treatment. The final extracts were prepared for analysis by HPLC. The results showed that degradation of both herbicides in the silty loam soil was faster than sandy loam soil. Half-life of sulfosulfuron was ranged from 5.37 to 10.82 days however this value for tribenuron methyl was ranged from 3.23 to 5.72 days on different soils. The residue of both herbicides at 120 days after application in wheat field had no toxicitic effect on lentil. It was concluded that HPLC analysis procedure was an appropriate method for determination of these herbicides from soils.

  3. 46 CFR 77.40-1 - Pilot boarding equipment.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 46 Shipping 3 2011-10-01 2011-10-01 false Pilot boarding equipment. 77.40-1 Section 77.40-1... MISCELLANEOUS SYSTEMS AND EQUIPMENT Pilot Boarding Equipment § 77.40-1 Pilot boarding equipment. (a) This section applies to each vessel that normally embarks or disembarks a pilot from a pilot boat or other...

  4. Measurement of Menadione in urine by HPLC

    USDA-ARS?s Scientific Manuscript database

    Menadione may be an important metabolite of vitamin K that is excreted in urine. A high performance liquid chromatography (HPLC) method with a C30 column, fluorescence detection and post-column zinc reduction was developed to measure menadione in urine. The mobile phase was composed of 95% methanol...

  5. An Inexpensive Digital Gradient Controller for HPLC.

    ERIC Educational Resources Information Center

    Brady, James E.; Carr, Peter W.

    1983-01-01

    Use of gradient elution techniques in high performance liquid chromatography (HPLC) is often essential for direct separation of complex mixtures. Since most commercial controllers have features that are of marginal value for instructional purposes, a low-cost controller capable of illustrating essential features of gradient elution was developed.…

  6. Surface confined ionic liquid as a stationary phase for HPLC

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Wang, Qian; Baker, Gary A; Baker, Sheila N

    Trimethoxysilane ionosilane derivatives of room temperature ionic liquids based on alkylimidazolium bromides were synthesized for attachment to silica support material. The derivatives 1-methyl-3-(trimethoxysilylpropyl)imidazolium bromide and 1-butyl-3-(trimethoxysilylpropyl)imidazolium bromide were used to modify the surface of 3 {micro}m diameter silica particles to act as the stationary phase for HPLC. The modified particles were characterized by thermogravimetric analysis (TGA) and {sup 13}C and {sup 29}Si NMR spectroscopies. The surface modification procedure rendered particles with a surface coverage of 0.84 {micro}mol m{sup -2} for the alkylimidazolium bromide. The ionic liquid moiety was predominantly attached to the silica surface through two siloxane bonds of themore » ionosilane derivative (63%). Columns packed with the modified silica material were tested under HPLC conditions. Preliminary evaluation of the stationary phase for HPLC was performed using aromatic carboxylic acids as model compounds. The separation mechanism appears to involve multiple interactions including ion exchange, hydrophobic interaction, and other electrostatic interactions.« less

  7. Quantitative determination of limonin in citrus juices by HPLC using computerized solvent optimization.

    PubMed

    Shaw, P E; Wilson, C W

    1988-09-01

    The commercially available computer program, Drylab, for optimization of separations by high-performance liquid chromatography (HPLC) using binary solvent mixtures is used to improve an HPLC method for separation of the bitter principle, limonin, in grapefruit and navel orange juices. Best conditions for separation of limonin in a reasonable time are 30 to 32% acetonitrile in water at 0.9 mL/min using a 5-micron C18 column 10 cm long. These conditions are used to analyze grapefruit and navel orange juice samples, and these HPLC results are compared with values determined by enzyme immunoassay or thin-layer chromatography (TLC) on the same samples.

  8. Safety analysis report for packaging, onsite, long-length contaminated equipment transport system

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    McCormick, W.A.

    1997-05-09

    This safety analysis report for packaging describes the components of the long-length contaminated equipment (LLCE) transport system (TS) and provides the analyses, evaluations, and associated operational controls necessary for the safe use of the LLCE TS on the Hanford Site. The LLCE TS will provide a standardized, comprehensive approach for the disposal of approximately 98% of LLCE scheduled to be removed from the 200 Area waste tanks.

  9. Determination of blood cyanide by HPLC-MS.

    PubMed

    Tracqui, A; Raul, J S; Géraut, A; Berthelon, L; Ludes, B

    2002-04-01

    An original high-performance liquid chromatographic-mass spectrometric (HPLC-MS) procedure was developed for the determination of cyanide (CN) in whole blood. After the addition of K13C15N as internal standard, blood was placed in a microdiffusion device, the inner well of which was filled with a mixture of taurine (50mM in water)/naphthalene-2,3-dicarboxaldehyde (NDA, 10mM in methanol)/methanol/ concentrated (approximately 20%) ammonia solution (25:25:45:5, v/v). Concentrated H2SO4 was added to the blood sample, and the microdiffusion chamber was sealed. After 30 min of gentle agitation, 2 microL of the contents of the inner vial were pipetted and directly injected onto a NovaPak C18 HPLC column. Separation was performed by a gradient of acetonitrile in 2mM NH4COOH, pH 3.0 buffer (35-80% in 10 min). Detection was done with a Perkin-Elmer Sciex API-100 mass analyzer with an ionspray interface, operated in the negative ionization mode. MS data were collected as either TIC or SIM at m/z (299 + 191) and (301 + 193) for the derivatives formed with CN and 13C15N, respectively. Inspired by previous works dealing with the complexation of CN by NDA + taurine to form a 1-cyano [f] benzoisoindole derivative analyzed by HPLC-fluorimetry, this method appears simple, rapid, and extremely specific. Limits of detection and quantitation for blood CN are 5 and 15 ng/mL, respectively. The use of 13C15N as internal standard allows the quantitation of CN with elegance and accuracy in comparison with previously reported methods.

  10. Direct characterization of aqueous extract of Hibiscus sabdariffa using HPLC with diode array detection coupled to ESI and ion trap MS.

    PubMed

    Rodríguez-Medina, Inmaculada C; Beltrán-Debón, Raúl; Molina, Vicente Micol; Alonso-Villaverde, Carlos; Joven, Jorge; Menéndez, Javier A; Segura-Carretero, Antonio; Fernández-Gutiérrez, Alberto

    2009-10-01

    The phenolic fraction and other polar compounds of the Hibiscus sabdariffa were separated and identified by HPLC with diode array detection coupled to electrospray TOF and IT tandem MS (DAD-HPLC-ESI-TOF-MS and IT-MS). The H. sabdariffa aqueous extract was filtered and directly injected into the LC system. The analysis of the compounds was carried out by RP HPLC coupled to DAD and TOF-MS in order to obtain molecular formula and exact mass. Posterior analyses with IT-MS were performed and the fragmentation pattern and confirmation of the structures were achieved. The H. sabdariffa samples were successfully analyzed in positive and negative ionization modes with two optimized linear gradients. In positive mode, the two most representative anthocyanins and other compounds were identified whereas the phenolic fraction, hydroxycitric acid and its lactone were identified using the negative ionization mode.

  11. Use of HPLC/UPLC-spectrophotometry for detection of formazan in in vitro Reconstructed human Tissue (RhT)-based test methods employing the MTT-reduction assay to expand their applicability to strongly coloured test chemicals.

    PubMed

    Alépée, N; Barroso, J; De Smedt, A; De Wever, B; Hibatallah, J; Klaric, M; Mewes, K R; Millet, M; Pfannenbecker, U; Tailhardat, M; Templier, M; McNamee, P

    2015-06-01

    A number of in vitro test methods using Reconstructed human Tissues (RhT) are regulatory accepted for evaluation of skin corrosion/irritation. In such methods, test chemical corrosion/irritation potential is determined by measuring tissue viability using the photometric MTT-reduction assay. A known limitation of this assay is possible interference of strongly coloured test chemicals with measurement of formazan by absorbance (OD). To address this, Cosmetics Europe evaluated use of HPLC/UPLC-spectrophotometry as an alternative formazan measurement system. Using the approach recommended by the FDA guidance for validation of bio-analytical methods, three independent laboratories established and qualified their HPLC/UPLC-spectrophotometry systems to reproducibly measure formazan from tissue extracts. Up to 26 chemicals were then tested in RhT test systems for eye/skin irritation and skin corrosion. Results support that: (1) HPLC/UPLC-spectrophotometry formazan measurement is highly reproducible; (2) formazan measurement by HPLC/UPLC-spectrophotometry and OD gave almost identical tissue viabilities for test chemicals not exhibiting colour interference nor direct MTT reduction; (3) independent of the test system used, HPLC/UPLC-spectrophotometry can measure formazan for strongly coloured test chemicals when this is not possible by absorbance only. It is therefore recommended that HPLC/UPLC-spectrophotometry to measure formazan be included in the procedures of in vitro RhT-based test methods, irrespective of the test system used and the toxicity endpoint evaluated to extend the applicability of these test methods to strongly coloured chemicals. Copyright © 2015 Elsevier Ltd. All rights reserved.

  12. Feasibility of Isolating Vulnerable Equipment of the Electric Power System from Sources of EMP

    DTIC Science & Technology

    1978-03-01

    Utilities, Colorado Springs, Colorado . Mr. Hugh Chastain, LaPlata Electric Association, Durango , Colcrado. Mr. Robert Kerger and Mr. James Ware...electric power systems, and (b) single-line diagrams for powar systems In the State of Colorado . 2. Determine the feanibility of manual isolation of...vulnerable equipment upon receipt ot’ attack warning. Feasibility should be studied in Colorado Syrings (risk area) and Fr-eemont County (host area) of

  13. Dipeptide Sequence Determination: Analyzing Phenylthiohydantoin Amino Acids by HPLC

    NASA Astrophysics Data System (ADS)

    Barton, Janice S.; Tang, Chung-Fei; Reed, Steven S.

    2000-02-01

    Amino acid composition and sequence determination, important techniques for characterizing peptides and proteins, are essential for predicting conformation and studying sequence alignment. This experiment presents improved, fundamental methods of sequence analysis for an upper-division biochemistry laboratory. Working in pairs, students use the Edman reagent to prepare phenylthiohydantoin derivatives of amino acids for determination of the sequence of an unknown dipeptide. With a single HPLC technique, students identify both the N-terminal amino acid and the composition of the dipeptide. This method yields good precision of retention times and allows use of a broad range of amino acids as components of the dipeptide. Students learn fundamental principles and techniques of sequence analysis and HPLC.

  14. [HPLC fingerprint of the antiarrhythmic fraction of Valeriana officinalis].

    PubMed

    Duan, Xue-Yun; Gong, Zhan-Feng; Chen, Shu-He; Fang, Ying; Liu, Yan-Wen

    2009-06-01

    To establish HPLC fingerprints of the Antiarrhythmic fraction of Valeriana officinalis. Agilent C18 (250 mm x 4.6 mm, 5 microm) column was used and the acetonitrile-water was chosen as the mobile phase in a gradient mode. The column temperature was 380 degrees C and the detection wavelength was 218 nm. The detection time was 70 min, and the flow rate was 1.0 mL/ min. Fifteen characteristic peaks were indicated in HPLC fingerprints. The relative retention time and the ranges of relative areas of the common peaks were also determined. This method is simple and accurate with a good reproducibility and provides a reference standard for the quality control of Valeriana officinalis.

  15. Equipment for pre-hospital airway management on Helicopter Emergency Medical System helicopters in central Europe.

    PubMed

    Schmid, M; Schüttler, J; Ey, K; Reichenbach, M; Trimmel, H; Mang, H

    2011-05-01

    For advanced out-of-hospital airway management, skilled personnel and adequate equipment are key prerequisites. There are little data on the current availability of airway management equipment and standards of medical staff on Helicopter Emergency Medical System (HEMS) helicopters in central Europe. An internet search identified all HEMS helicopters in Austria, Switzerland and Luxembourg. We identified 15 HEMS helicopter bases in Switzerland, 28 in Austria and three in Luxembourg. A questionnaire was sent to all bases, asking both for the details of the clinical background and experience of participating staff, and details of airway management equipment carried routinely on board. Replies were received from 14 helicopter bases in Switzerland (93%), 25 bases in Austria (89%) and all three bases in Luxembourg. Anaesthesiologists were by far the most frequent attending physicians (68-85%). All except one bases reported to have at least one alternative supraglottic airway device. All bases had capnometry and succinylcholine. All bases in the study except two in Austria had commercial pre-packed sets for a surgical airway. All helicopters were equipped with automatic ventilators, although not all were suitable for non-invasive ventilation (NIV; Switzerland: 43%, Austria: 12%, Luxembourg: 100%). Masks for NIV were rarely available in Switzerland (two bases; 14%) and in Austria (three bases; 12%), whereas all three bases in Luxembourg carried those masks. Most HEMS helicopters carry appropriate equipment to meet the demands of modern advanced airway management in the pre-hospital setting. Further work is needed to ensure that appropriate airway equipment is carried on all HEMS helicopters.

  16. Chemical fingerprinting and quantitative constituent analysis of Siwu decoction categorized formulae by UPLC-QTOF/MS/MS and HPLC-DAD

    PubMed Central

    2013-01-01

    Background Siwu decoction categorized formulae (SWDCF) are widely used for treating gynecological diseases. This study aims to elucidate the differences of bioactive constituents in SWDCF by ultra-high performance liquid chromatography coupled with time-of-flight mass spectrometry (UPLC - QTOF - MS /MS) and HPLC-DAD. Methods An efficient method based on UPLC - QTOF - MS /MS was developed for identifying the chemical profiles of SWDCF. HPLC-DAD method was used for quantifying seven chemical markers in SWDCF. Results Eighty four components were identified or characterized, including ten organic acids, thirty glycosides (monoterpene or iridoid or phenylpropanoids glycosides), fourteen lactones, eighteen flavonoids, and eleven alkaloids in the complex system. The datasets of tR-m/z pairs, ion intensities and sample codes were processed with supervised orthogonal partial least squared discriminant analysis to compare these decoction samples. After a clear classification was established, OPLS-DA was performed and 16 common components with relative quantity in SWDCF samples were determined. Gallic acid, protocatechuic acid, vanillic acid, caffeic acid, paeoniflorin, ferulic acid, and senkyunolide I were selected as the chemical markers to identify SWDCF by HPLC-DAD. Conclusion The chemical profiles with 84 components in SWDCF, including monoterpene glycosides, acetophenones, galloyl glucoses, even some isomers in the complex system were characterized by UPLC–QTOF–MS/MS. PMID:23453004

  17. Control of equipment isolation system using wavelet-based hybrid sliding mode control

    NASA Astrophysics Data System (ADS)

    Huang, Shieh-Kung; Loh, Chin-Hsiung

    2017-04-01

    Critical non-structural equipment, including life-saving equipment in hospitals, circuit breakers, computers, high technology instrumentations, etc., is vulnerable to strong earthquakes, and on top of that, the failure of the vibration-sensitive equipment will cause severe economic loss. In order to protect vibration-sensitive equipment or machinery against strong earthquakes, various innovative control algorithms are developed to compensate the internal forces that to be applied. These new or improved control strategies, such as the control algorithms based on optimal control theory and sliding mode control (SMC), are also developed for structures engineering as a key element in smart structure technology. The optimal control theory, one of the most common methodologies in feedback control, finds control forces through achieving a certain optimal criterion by minimizing a cost function. For example, the linear-quadratic regulator (LQR) was the most popular control algorithm over the past three decades, and a number of modifications have been proposed to increase the efficiency of classical LQR algorithm. However, except to the advantage of simplicity and ease of implementation, LQR are susceptible to parameter uncertainty and modeling error due to complex nature of civil structures. Different from LQR control, a robust and easy to be implemented control algorithm, SMC has also been studied. SMC is a nonlinear control methodology that forces the structural system to slide along surfaces or boundaries; hence this control algorithm is naturally robust with respect to parametric uncertainties of a structure. Early attempts at protecting vibration-sensitive equipment were based on the use of existing control algorithms as described above. However, in recent years, researchers have tried to renew the existing control algorithms or developing a new control algorithm to adapt the complex nature of civil structures which include the control of both structures and non

  18. Development of a Portable Sensitive Equipment Decontamination System. Volume 1: Commercial Candidates Materials Evaluation (Chemical Agent Studies)

    DTIC Science & Technology

    2010-05-01

    decontaminate chemical and biological agents from sensitive equipment (avionics, electronics, electrical , and environmental systems and equipment...fabricated 2 x 2 in. square, 3/32 in. thick aluminum shims, augmented with electrical tape for added thickness as needed, were used in these tests to make...test coupons, thin custom-fabricated 2x2 in. square x 3/32 in. thick aluminum shims, augmented with electrical tape for added thickness as needed

  19. [Relationship between HPLC fingerprint chromatogram and inhibitory effect on respiratory burst of rat PMN of leaves of crataegus].

    PubMed

    Liu, Rong-hua; Yu, Bo-yang; Chen, Lan-ying; Liu, Ji-hua; Shao, Feng; Ma, Zhi-lin; Yang, Ming

    2008-08-01

    To study the relationship between HPLC fingerprint chromatogram and inhibitory effect on respiratory burst of rat PMN of leaves of crataegus L. HPLC fingerprint peaks of different species of hawthorn leaves were isolated and used for the effective experiment on the respiratory burst of rat PMN. The mathematic models of the relationship between the area and the effect of fingerprint peaks were established. According to the mathematic models, the HPLC fingerprint were change into bioactive fingerprint (include effective fingerprint and potency fingerprint) with the helps of mathematics, chemometrics, computer program simulation and etc. The chromatogram-effect relationship of leaves of crataegus. on respiratory burst of rat PMN was established. According to this relationship, the activities of fourteen samples of leaves of crataegus. were forecasted. It was positive correlation between the expected value and the practical value. And the correlation coefficients was 0.968 (P < 0.01). An all-around evaluative system, which includes not only chemical identification but also effective evaluation for traditional Chinese medicine was established. It will provide a new idea for study on fingerprint chromatogram of traditional Chinese medicine.

  20. 46 CFR 129.370 - Equipment grounding.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 46 Shipping 4 2012-10-01 2012-10-01 false Equipment grounding. 129.370 Section 129.370 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) OFFSHORE SUPPLY VESSELS ELECTRICAL INSTALLATIONS Power Sources and Distribution Systems § 129.370 Equipment grounding. (a) On a metallic vessel...

  1. 46 CFR 129.370 - Equipment grounding.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 46 Shipping 4 2014-10-01 2014-10-01 false Equipment grounding. 129.370 Section 129.370 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) OFFSHORE SUPPLY VESSELS ELECTRICAL INSTALLATIONS Power Sources and Distribution Systems § 129.370 Equipment grounding. (a) On a metallic vessel...

  2. 46 CFR 129.370 - Equipment grounding.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 46 Shipping 4 2013-10-01 2013-10-01 false Equipment grounding. 129.370 Section 129.370 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) OFFSHORE SUPPLY VESSELS ELECTRICAL INSTALLATIONS Power Sources and Distribution Systems § 129.370 Equipment grounding. (a) On a metallic vessel...

  3. Design and realization of test system for testing parallelism and jumpiness of optical axis of photoelectric equipment

    NASA Astrophysics Data System (ADS)

    Shi, Sheng-bing; Chen, Zhen-xing; Qin, Shao-gang; Song, Chun-yan; Jiang, Yun-hong

    2014-09-01

    With the development of science and technology, photoelectric equipment comprises visible system, infrared system, laser system and so on, integration, information and complication are higher than past. Parallelism and jumpiness of optical axis are important performance of photoelectric equipment,directly affect aim, ranging, orientation and so on. Jumpiness of optical axis directly affect hit precision of accurate point damage weapon, but we lack the facility which is used for testing this performance. In this paper, test system which is used fo testing parallelism and jumpiness of optical axis is devised, accurate aim isn't necessary and data processing are digital in the course of testing parallelism, it can finish directly testing parallelism of multi-axes, aim axis and laser emission axis, parallelism of laser emission axis and laser receiving axis and first acuualizes jumpiness of optical axis of optical sighting device, it's a universal test system.

  4. Feasibility of high recovery highwall mining equipment. Final report

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Not Available

    1981-01-01

    Three equipment systems exhibited significant promise: the RSV Miner, a surface longwall using standard underground equipment, and the variable angle auger. Other equipment systems showing considerable merit were the surface shortwall, and the two extended depth augers. Of the three most significant systems, the RSV Miner exhibits the greatest versatility and adaptability. It may be used competently in many surface mining applications and readily adapts to geologic anomalies and changing seam heights. The machine employs steering and guidance equipment and provides the necessary capabilities for extended depth operation. Safety is good, as no men are required to work underground. However,more » most important is the system's recovery factor of approximately 75% to 80% of the in-situ coal reserve within reach. The surface longwall system using standard underground equipment (preferably a ranging drum shearer in conjunction with shield supports) is most suited to either a trench mining or a modified area mining application. Both applications would allow the length of the face to be held constant. Another important consideration is legal requirements for a tailgate entry, which would necessitate additional equipment for development in a modified area mining application. When compared to surface shortwall, surface longwall exhibits higher productivity, a far greater equipment selection which allows system tailoring to geologic conditions, and greater roof control due to the significantly smaller section of overburden that must be supported. Recovery should approach, and possibly exceed, 90% of the coal in-place. The variable angle auger, which is currently only a concept, fills a very real need for which no other equipment is available at this time.« less

  5. Determination and validation of six sunscreen agents in suncare products by UPLC and HPLC.

    PubMed

    Lee, So-Mi; Jeong, Hye-Jin; Chang, Ih Seop

    2008-01-01

    Methylene bis-benzotriazolyl tetramethyl butylphenol and bis-ethylhexyloxy phenol methoxyphenyl triazine are sunscreen agents that have hydrophobic behaviors in common. They were not normally assayed with the following four sunscreen agents that have hydrophilic behaviors in a single chromatographic run: ethylhexyl methoxycinnamate, isoamyl p-methoxycinnamate, ethylhexyl salicylate, and ethylhexyl triazone. For that reason, methylene bis-benzotriazolyl tetramethyl butylphenol and bis-ethylhexyloxy phenol methoxyphenyl triazine require much time in order to assay products with those materials. A rapid, selective, and reproducible determination method needs to be developed for the simultaneous examination of methylene bis-benzotriazolyl tetramethyl butylphenol and bis-ethylhexyloxy phenol methoxyphenyl triazine with the sunscreen agents, ethylhexyl methoxycinnamate, isoamyl p-methoxycinnamate, ethylhexyl salicylate, and ethylhexyl triazone. This new technique could reduce time in examining the sunscreen agents and be effective for quality control of suncare products. In this paper, the HPLC and UPLC system is used for developing the determination of the sunscreen agents. Several evaluations of some mixtures of eluents and columns were obtained for the optimal condition of separation. In HPLC, the optimal peak resolution was obtained through ethanol-water gradient elution and a 75-mm C18 column with a 3.5-microm-sized particle on a flow rate of 1.0 ml/min. In UPLC, the most distinctive peak resolution was obtained through methanol-water gradient elution and a 50-mm C18 column with a 1.7-microm-sized particle on a flow rate 0.4 ml/min. Both of those chromatographic determination methods could be used in the examination of six types of sunscreen agents without any interference from other product excipients in the agents. The proposed determination methods were validated for specificity, linearity, repeatability, system stability, intermediate precision, and accuracy

  6. 48 CFR 970.1504-1-8 - Special equipment purchases.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... boilers, generators, machine tools, and large electrical equipment. In some cases, it would also include... 48 Federal Acquisition Regulations System 5 2010-10-01 2010-10-01 false Special equipment....1504-1-8 Special equipment purchases. (a) Special equipment is sometimes procured in conjunction with...

  7. Assessment of cosmetic ingredients in the in vitro reconstructed human epidermis test method EpiSkin™ using HPLC/UPLC-spectrophotometry in the MTT-reduction assay.

    PubMed

    Alépée, N; Hibatallah, J; Klaric, M; Mewes, K R; Pfannenbecker, U; McNamee, P

    2016-06-01

    Cosmetics Europe recently established HPLC/UPLC-spectrophotometry as a suitable alternative endpoint detection system for measurement of formazan in the MTT-reduction assay of reconstructed human tissue test methods irrespective of the test system involved. This addressed a known limitation for such test methods that use optical density for measurement of formazan and may be incompatible for evaluation of strong MTT reducer and/or coloured chemicals. To build on the original project, Cosmetics Europe has undertaken a second study that focuses on evaluation of chemicals with functionalities relevant to cosmetic products. Such chemicals were primarily identified from the Scientific Committee on Consumer Safety (SCCS) 2010 memorandum (addendum) on the in vitro test EpiSkin™ for skin irritation testing. Fifty test items were evaluated in which both standard photometry and HPLC/UPLC-spectrophotometry were used for endpoint detection. The results obtained in this study: 1) provide further support for Within Laboratory Reproducibility of HPLC-UPLC-spectrophotometry for measurement of formazan; 2) demonstrate, through use a case study with Basazol C Blue pr. 8056, that HPLC/UPLC-spectrophotometry enables determination of an in vitro classification even when this is not possible using standard photometry and 3) addresses the question raised by SCCS in their 2010 memorandum (addendum) to consider an endpoint detection system not involving optical density quantification in in vitro reconstructed human epidermis skin irritation test methods. Copyright © 2016 Elsevier Ltd. All rights reserved.

  8. 46 CFR 108.437 - Pipe sizes and discharge rates for enclosed ventilation systems for rotating electrical equipment.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 46 Shipping 4 2011-10-01 2011-10-01 false Pipe sizes and discharge rates for enclosed ventilation... Systems Fixed Carbon Dioxide Fire Extinguishing Systems § 108.437 Pipe sizes and discharge rates for enclosed ventilation systems for rotating electrical equipment. (a) The minimum pipe size for the initial...

  9. 46 CFR 108.437 - Pipe sizes and discharge rates for enclosed ventilation systems for rotating electrical equipment.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 46 Shipping 4 2010-10-01 2010-10-01 false Pipe sizes and discharge rates for enclosed ventilation systems for rotating electrical equipment. 108.437 Section 108.437 Shipping COAST GUARD, DEPARTMENT OF... Systems Fixed Carbon Dioxide Fire Extinguishing Systems § 108.437 Pipe sizes and discharge rates for...

  10. Investigating Pigment Radicals in Black Rice Using HPLC and Multi-EPR.

    PubMed

    Nakagawa, Kouichi; Maeda, Hayato

    2017-01-01

    We investigated the location and distribution of paramagnetic species in black and white rice using electron paramagnetic resonance (EPR), X-band (9 GHz) EPR imaging (EPRI), and HPLC. EPR primarily detected two paramagnetic species in black rice, which were identified as a stable radical and Mn 2+ species, based on the g values and hyperfine components of the EPR signals. The signal from the stable radical appeared at g ≈ 2.00 and was relatively strong and stable. Subsequent noninvasive two-dimensional (2D) EPRI revealed that this stable radical was primarily located in the pigmented region of black rice, while very few radicals were observed in the rice interior. Pigments extracted from black rice were analyzed using HPLC; the major compound was found to be cyanidin-3-glucoside. EPR and HPLC results indicate that the stable radical was only found within the pigmented region of the rice, and that it could either be cyanidin-3-glucoside, or one of its oxidative decomposition products.

  11. Screening natural antioxidants in peanut shell using DPPH-HPLC-DAD-TOF/MS methods.

    PubMed

    Qiu, Jiying; Chen, Leilei; Zhu, Qingjun; Wang, Daijie; Wang, Wenliang; Sun, Xin; Liu, Xiaoyong; Du, Fangling

    2012-12-15

    Peanut shell, a byproduct in oil production, is rich in natural antioxidants. Here, a rapid and efficient method using DPPH-HPLC-DAD-TOF/MS was used for the first time to screen antioxidants in peanut shell. The method is based on the hypothesis that upon reaction with 1, 1-diphenyl-2-picrylhydrazyl (DPPH), the peak areas of compounds with potential antioxidant activities in the HPLC chromatogram will be significantly reduced or disappeared, and the identity confirmation could be achieved by HPLC-DAD-TOF/MS technique. With this method, three compounds possessing potential antioxidant activities were found abundantly in the methanolic extract of peanut shell. They were identified as 5,7-dihydroxychromone, eriodictyol, and luteolin. The contents of these compounds were 0.59, 0.92, and 2.36 mg/g, respectively, and luteolin possessed the strongest radical scavenging capacity. DPPH-HPLC-DAD-TOF/MS assay facilitated rapid identification and determination of natural antioxidants in peanut shell, which may be helpful for value-added utilization of peanut processing byproducts. Copyright © 2012 Elsevier Ltd. All rights reserved.

  12. 46 CFR 189.25-30 - Electrical engineering equipment.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 46 Shipping 7 2011-10-01 2011-10-01 false Electrical engineering equipment. 189.25-30 Section 189... VESSELS INSPECTION AND CERTIFICATION Inspection for Certification § 189.25-30 Electrical engineering equipment. (a) For inspection procedures of Electrical Engineering equipment and systems, see Subchapter J...

  13. 46 CFR 91.25-30 - Electrical engineering equipment.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 46 Shipping 4 2014-10-01 2014-10-01 false Electrical engineering equipment. 91.25-30 Section 91.25... INSPECTION AND CERTIFICATION Inspection for Certification § 91.25-30 Electrical engineering equipment. For inspection procedures of electrical engineering equipment and systems see subchapter J (Electrical...

  14. 46 CFR 91.25-30 - Electrical engineering equipment.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 46 Shipping 4 2010-10-01 2010-10-01 false Electrical engineering equipment. 91.25-30 Section 91.25... INSPECTION AND CERTIFICATION Inspection for Certification § 91.25-30 Electrical engineering equipment. For inspection procedures of electrical engineering equipment and systems see subchapter J (Electrical...

  15. 46 CFR 91.25-30 - Electrical engineering equipment.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 46 Shipping 4 2011-10-01 2011-10-01 false Electrical engineering equipment. 91.25-30 Section 91.25... INSPECTION AND CERTIFICATION Inspection for Certification § 91.25-30 Electrical engineering equipment. For inspection procedures of electrical engineering equipment and systems see subchapter J (Electrical...

  16. 46 CFR 91.25-30 - Electrical engineering equipment.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 46 Shipping 4 2013-10-01 2013-10-01 false Electrical engineering equipment. 91.25-30 Section 91.25... INSPECTION AND CERTIFICATION Inspection for Certification § 91.25-30 Electrical engineering equipment. For inspection procedures of electrical engineering equipment and systems see subchapter J (Electrical...

  17. 46 CFR 189.25-30 - Electrical engineering equipment.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 46 Shipping 7 2010-10-01 2010-10-01 false Electrical engineering equipment. 189.25-30 Section 189... VESSELS INSPECTION AND CERTIFICATION Inspection for Certification § 189.25-30 Electrical engineering equipment. (a) For inspection procedures of Electrical Engineering equipment and systems, see Subchapter J...

  18. 46 CFR 189.25-30 - Electrical engineering equipment.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 46 Shipping 7 2012-10-01 2012-10-01 false Electrical engineering equipment. 189.25-30 Section 189... VESSELS INSPECTION AND CERTIFICATION Inspection for Certification § 189.25-30 Electrical engineering equipment. (a) For inspection procedures of Electrical Engineering equipment and systems, see Subchapter J...

  19. 46 CFR 189.25-30 - Electrical engineering equipment.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 46 Shipping 7 2014-10-01 2014-10-01 false Electrical engineering equipment. 189.25-30 Section 189... VESSELS INSPECTION AND CERTIFICATION Inspection for Certification § 189.25-30 Electrical engineering equipment. (a) For inspection procedures of Electrical Engineering equipment and systems, see Subchapter J...

  20. 46 CFR 189.25-30 - Electrical engineering equipment.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 46 Shipping 7 2013-10-01 2013-10-01 false Electrical engineering equipment. 189.25-30 Section 189... VESSELS INSPECTION AND CERTIFICATION Inspection for Certification § 189.25-30 Electrical engineering equipment. (a) For inspection procedures of Electrical Engineering equipment and systems, see Subchapter J...

  1. 46 CFR 91.25-30 - Electrical engineering equipment.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 46 Shipping 4 2012-10-01 2012-10-01 false Electrical engineering equipment. 91.25-30 Section 91.25... INSPECTION AND CERTIFICATION Inspection for Certification § 91.25-30 Electrical engineering equipment. For inspection procedures of electrical engineering equipment and systems see subchapter J (Electrical...

  2. Predicting equipment needs of children with cerebral palsy using the Gross Motor Function Classification System: a cross-sectional study.

    PubMed

    Novak, Iona; Smithers-Sheedy, Hayley; Morgan, Cathy

    2012-01-01

    Children with cerebral palsy (CP) routinely use assistive equipment to improve their independence. Specialist equipment is expensive and therefore not always available to the child when needed. The aim of this study was to determine whether the assistive equipment needs of children with CP and the associated costs could be predicted. A cross-sectional study using a chart audit was completed. Two hundred forty-two children met eligibility criteria and were included in the study. Data abstracted from files pertained to the child's CP, associated impairments and assistive equipment prescribed. The findings were generated using linear regression modelling. Gross Motor Function Classification System (GMFCS) level [B = 3.01 (95% CI, 2.36-3.57), p = 0.000] and the presence of epilepsy [B = 2.35 (95% CI, 0.64-4.06), p = 0.008] predicted the prescription of assistive equipment. The more severely affected the gross motor function impairment, the more equipment that was required and the more the equipment cost. The equipment needs of children with CP can be predicted for the duration of childhood. This information may be useful for families and for budget and service planning.

  3. Microwave-assisted extraction with water for fast extraction and simultaneous RP-HPLC determination of phenolic acids in radix Salviae Miltiorrhizae.

    PubMed

    Fang, Xinsheng; Wang, Jianhua; Zhou, Hongying; Jiang, Xingkai; Zhu, Lixiang; Gao, Xin

    2009-07-01

    An optimized microwave-assisted extraction method using water (MAE-W) as the extractant and an efficient HPLC analysis method were first developed for the fast extraction and simultaneous determination of D(+)-(3,4-dihydroxyphenyl) lactic acid (Dla), salvianolic acid B (SaB), and lithospermic acid (La) in radix Salviae Miltiorrhizae. The key parameters of MAE-W were optimized. It was found that the degradation of SaB was inhibited when using the optimized MAE-W and the stable content of Dla, La, and SaB in danshen was obtained. Furthermore, compared to the conventional extraction methods, the proposed MAE-W is a more rapid method with higher yield and lower solvent consumption with a reproducibility (RSD <6%). In addition, using water as extractant is safe and helpful for environment protection, which could be referred to as green extraction. The separation and quantitative determination of the three compounds was carried out by a developed reverse-phase high-performance liquid chromatographic (RP-HPLC) method with UV detection. Highly efficient separation was obtained using gradient solvent system. The optimized HPLC analysis method was validated to have specificity, linearity, precision, and accuracy. The results indicated that MAE-W followed by HPLC-UV determination is an appropriate alternative to previously proposed method for quality control of radix Salviae Miltiorrhizae.

  4. Effectiveness of medical equipment donations to improve health systems: how much medical equipment is broken in the developing world?

    PubMed

    Perry, Lora; Malkin, Robert

    2011-07-01

    It is often said that most of the medical equipment in the developing world is broken with estimates ranging up to 96% out of service. But there is little documented evidence to support these statements. We wanted to quantify the amount of medical equipment that was out of service in resource poor health settings and identify possible causes. Inventory reports were analyzed from 1986 to 2010, from hospitals in sixteen countries across four continents. The UN Human Development Index was used to determine which countries should be considered developing nations. Non-medical hospital equipment was excluded. This study examined 112,040 pieces of equipment. An average of 38.3% (42,925, range across countries: 0.83-47%) in developing countries was out of service. The three main causes were lack of training, health technology management, and infrastructure. We hope that the findings will help biomedical engineers with their efforts toward effective designs for the developing world and NGO's with efforts to design effective healthcare interventions.

  5. Application and preventive maintenance of neurology medical equipment in isfahan alzahra hospital.

    PubMed

    Alikhani, Parivash; Vesal, Sahar; Kashefi, Parviz; Pour, Ramin Etamadi; Khorvash, Fariborz; Askari, Gholamreza; Meamar, Rokhsareh

    2013-05-01

    Nowadays Medical equipment plays an important role in the treatment and in the medical education. Using outdated preventive maintenance (PM) system may cause problems in the cutting edge medical equipment, Nervous system disease's equipment (In diagnosis and treatment) which are crucial for every medical center. Based on above facts we focused on nervous system treat units' equipment and informed the supervisors and their colleagues about the latest equipment maintenance status and promoted methodical and correct method to be used for medical equipment maintenance. This research is an analytical descriptive and has been done on the base information from a particular time to past. We gathered our required information of 2009 from Alzahra Medical Center. We divided this research info 2 main phases. In the first phase, we picked out Neurosurgery and Neourology diseases medical equipment (diagnosis and therapy equipment) and in the second phase, we need to implement a methodical PM for every equipment. Research has shown that there are 19nervous system equipment in Alzahra Medical center, categorized in diagnostic (13 pcs), therapeutic (4 pcs) and diagnostic-therapeutic (2 pcs). As we declare in methods part of this research, we categorized medical equipment in Food and Drug Administration (FDA) segmentation. Capital-scarce equipment: Magnetic resonance imaging, Eco Doppler, Kamalaarak ultrasonic surgical aspirator, Stereotactic, computed tomography-scan, euroendoscope/vital-scarce equipment: Coblation, Sonoco, vaterjet/scarce equipment: Transcranial color Doppler, electroencephalogram, electromyography, surgical microscope. Survey of application and preventive maintenance of neurology medical equipment in Isfahan Alzahra hospital show there is no P.M system. Implementing a complete P.M system for this medical center is crucial to preventing cause problems for these medical equipment and decreasing maintenance costs and gaining uptime. Researchers of this article have

  6. Estimation of color of durum wheat. Comparison of WSB, HPLC, and reflectance colorimeter measurements.

    PubMed

    Fratianni, Alessandra; Irano, Mario; Panfili, Gianfranco; Acquistucci, Rita

    2005-04-06

    Color is an important parameter involved in the definition of semolina and pasta quality. This character is mainly due to natural pigments (carotenoids) that are present at different levels in cereals and cereal products, due to botanical origin, growing conditions, distribution in the kernel, and technological processes. In food industries, color measurements are usually performed by means of automatic instruments that are rapid and safe, as alternatives to the chemical extraction methods. In this study, automatic measurements (CIE, color-space system L, a, b), water-saturated butanol (WSB), and HPLC determinations have been applied to evaluate the carotenoid content in whole meals and respective semolina samples produced from wheat cultivated in the years 2001 and 2002. In whole meals, total carotenoids, determined by HPLC, were about 3.0 microg/g (2001) and 3.5 microg/g (2002) calculated on dry weight (dw) and about 3.0 and 3.2 microg/g dw in corresponding semolina samples. The b values for the same period were 19.78 and 15.75, respectively, in raw materials and 20.03-21.67 in semolina. Results have confirmed lutein and beta-carotene as the main components mainly responsible for the yellow color in wheat grains. The ability of the index b to express natural dyeing was dependent on sample characteristics as demonstrated by the relationships found between this index and pigments, although the best correlation resulted between HPLC and WSB.

  7. Extraction and Determination of Cyproheptadine in Human Urine by DLLME-HPLC Method

    PubMed Central

    Maham, Mehdi; Kiarostami, Vahid; Waqif-Husain, Syed; Abroomand-Azar, Parviz; Tehrani, Mohammad Saber; Khoeini Sharifabadi, Malihe; Afrouzi, Hossein; Shapouri, MahmoudReza; Karami-Osboo, Rouhollah

    2013-01-01

    Novel dispersive liquid-liquid microextraction (DLLME), coupled with high performance liquid chromatography with photodiode array detection (HPLC-DAD) has been applied for the extraction and determination of cyproheptadine (CPH), an antihistamine, in human urine samples. In this method, 0.6 mL of acetonitrile (disperser solvent) containing 30 μL of carbon tetrachloride (extraction solvent) was rapidly injected by a syringe into 5 mL urine sample. After centrifugation, the sedimented phase containing enriched analyte was dissolved in acetonitrile and an aliquot of this solution injected into the HPLC system for analysis. Development of DLLME procedure includes optimization of some important parameters such as kind and volume of extraction and disperser solvent, pH and salt addition. The proposed method has good linearity in the range of 0.02-4.5 μg mL-1 and low detection limit (13.1 ng mL-1). The repeatability of the method, expressed as relative standard deviation was 4.9% (n = 3). This method has also been applied to the analysis of real urine samples with satisfactory relative recoveries in the range of 91.6-101.0%. PMID:24250605

  8. Extraction and Determination of Cyproheptadine in Human Urine by DLLME-HPLC Method.

    PubMed

    Maham, Mehdi; Kiarostami, Vahid; Waqif-Husain, Syed; Abroomand-Azar, Parviz; Tehrani, Mohammad Saber; Khoeini Sharifabadi, Malihe; Afrouzi, Hossein; Shapouri, Mahmoudreza; Karami-Osboo, Rouhollah

    2013-01-01

    Novel dispersive liquid-liquid microextraction (DLLME), coupled with high performance liquid chromatography with photodiode array detection (HPLC-DAD) has been applied for the extraction and determination of cyproheptadine (CPH), an antihistamine, in human urine samples. In this method, 0.6 mL of acetonitrile (disperser solvent) containing 30 μL of carbon tetrachloride (extraction solvent) was rapidly injected by a syringe into 5 mL urine sample. After centrifugation, the sedimented phase containing enriched analyte was dissolved in acetonitrile and an aliquot of this solution injected into the HPLC system for analysis. Development of DLLME procedure includes optimization of some important parameters such as kind and volume of extraction and disperser solvent, pH and salt addition. The proposed method has good linearity in the range of 0.02-4.5 μg mL(-1) and low detection limit (13.1 ng mL(-1)). The repeatability of the method, expressed as relative standard deviation was 4.9% (n = 3). This method has also been applied to the analysis of real urine samples with satisfactory relative recoveries in the range of 91.6-101.0%.

  9. Advanced deep sea diving equipment

    NASA Technical Reports Server (NTRS)

    Danesi, W. A.

    1972-01-01

    Design requirements are generated for a deep sea heavy duty diving system to equip salvage divers with equipment and tools that permit work of the same quality and in times approaching that done on the surface. The system consists of a helmet, a recirculator for removing carbon dioxide, and the diver's dress. The diver controls the inlet flow by the recirculatory control valve and is able to change closed cycle operation to open cycle if malfunction occurs. Proper function of the scrubber in the recirculator minimizes temperature and humidity effects as it filters the returning air.

  10. Standards for material handling and facilities equipment proofload testing

    NASA Technical Reports Server (NTRS)

    Bonn, S. P.

    1970-01-01

    Document provides information on verifying the safety of material handling and facilities equipment /MH/FE/, ranging from monorail systems to ladders and non-powered mobile equipment. Seven catagories of MH/FE equipment are defined.

  11. 46 CFR 130.220 - Design of equipment for cooking and heating.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 46 Shipping 4 2012-10-01 2012-10-01 false Design of equipment for cooking and heating. 130.220 Section 130.220 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) OFFSHORE SUPPLY VESSELS VESSEL CONTROL, AND MISCELLANEOUS EQUIPMENT AND SYSTEMS Miscellaneous Equipment and Systems § 130.220 Design of equipment for cooking and heating....

  12. 46 CFR 130.220 - Design of equipment for cooking and heating.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 46 Shipping 4 2014-10-01 2014-10-01 false Design of equipment for cooking and heating. 130.220 Section 130.220 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) OFFSHORE SUPPLY VESSELS VESSEL CONTROL, AND MISCELLANEOUS EQUIPMENT AND SYSTEMS Miscellaneous Equipment and Systems § 130.220 Design of equipment for cooking and heating....

  13. 46 CFR 111.01-3 - Placement of equipment.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 46 Shipping 4 2010-10-01 2010-10-01 false Placement of equipment. 111.01-3 Section 111.01-3 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) ELECTRICAL ENGINEERING ELECTRIC SYSTEMS-GENERAL REQUIREMENTS General § 111.01-3 Placement of equipment. (a) Electric equipment must be arranged...

  14. 46 CFR 111.01-3 - Placement of equipment.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 46 Shipping 4 2014-10-01 2014-10-01 false Placement of equipment. 111.01-3 Section 111.01-3 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) ELECTRICAL ENGINEERING ELECTRIC SYSTEMS-GENERAL REQUIREMENTS General § 111.01-3 Placement of equipment. (a) Electric equipment must be arranged...

  15. 46 CFR 111.01-3 - Placement of equipment.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 46 Shipping 4 2011-10-01 2011-10-01 false Placement of equipment. 111.01-3 Section 111.01-3 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) ELECTRICAL ENGINEERING ELECTRIC SYSTEMS-GENERAL REQUIREMENTS General § 111.01-3 Placement of equipment. (a) Electric equipment must be arranged...

  16. 46 CFR 111.01-3 - Placement of equipment.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 46 Shipping 4 2013-10-01 2013-10-01 false Placement of equipment. 111.01-3 Section 111.01-3 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) ELECTRICAL ENGINEERING ELECTRIC SYSTEMS-GENERAL REQUIREMENTS General § 111.01-3 Placement of equipment. (a) Electric equipment must be arranged...

  17. 46 CFR 189.25-35 - Marine engineering equipment.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 46 Shipping 7 2014-10-01 2014-10-01 false Marine engineering equipment. 189.25-35 Section 189.25... INSPECTION AND CERTIFICATION Inspection for Certification § 189.25-35 Marine engineering equipment. (a) For inspection procedures of Marine Engineering equipment and systems, see Subchapter F (Marine Engineering) of...

  18. 46 CFR 91.25-35 - Marine engineering equipment.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 46 Shipping 4 2014-10-01 2014-10-01 false Marine engineering equipment. 91.25-35 Section 91.25-35... INSPECTION AND CERTIFICATION Inspection for Certification § 91.25-35 Marine engineering equipment. (a) For inspection procedures of marine engineering equipment and systems, see subchapter F (Marine Engineering) of...

  19. 46 CFR 91.25-35 - Marine engineering equipment.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 46 Shipping 4 2011-10-01 2011-10-01 false Marine engineering equipment. 91.25-35 Section 91.25-35... INSPECTION AND CERTIFICATION Inspection for Certification § 91.25-35 Marine engineering equipment. (a) For inspection procedures of marine engineering equipment and systems, see subchapter F (Marine Engineering) of...

  20. 46 CFR 189.25-35 - Marine engineering equipment.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 46 Shipping 7 2013-10-01 2013-10-01 false Marine engineering equipment. 189.25-35 Section 189.25... INSPECTION AND CERTIFICATION Inspection for Certification § 189.25-35 Marine engineering equipment. (a) For inspection procedures of Marine Engineering equipment and systems, see Subchapter F (Marine Engineering) of...

  1. 46 CFR 91.25-35 - Marine engineering equipment.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 46 Shipping 4 2013-10-01 2013-10-01 false Marine engineering equipment. 91.25-35 Section 91.25-35... INSPECTION AND CERTIFICATION Inspection for Certification § 91.25-35 Marine engineering equipment. (a) For inspection procedures of marine engineering equipment and systems, see subchapter F (Marine Engineering) of...

  2. 46 CFR 189.25-35 - Marine engineering equipment.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 46 Shipping 7 2011-10-01 2011-10-01 false Marine engineering equipment. 189.25-35 Section 189.25... INSPECTION AND CERTIFICATION Inspection for Certification § 189.25-35 Marine engineering equipment. (a) For inspection procedures of Marine Engineering equipment and systems, see Subchapter F (Marine Engineering) of...

  3. 46 CFR 189.25-35 - Marine engineering equipment.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 46 Shipping 7 2012-10-01 2012-10-01 false Marine engineering equipment. 189.25-35 Section 189.25... INSPECTION AND CERTIFICATION Inspection for Certification § 189.25-35 Marine engineering equipment. (a) For inspection procedures of Marine Engineering equipment and systems, see Subchapter F (Marine Engineering) of...

  4. 46 CFR 91.25-35 - Marine engineering equipment.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 46 Shipping 4 2010-10-01 2010-10-01 false Marine engineering equipment. 91.25-35 Section 91.25-35... INSPECTION AND CERTIFICATION Inspection for Certification § 91.25-35 Marine engineering equipment. (a) For inspection procedures of marine engineering equipment and systems, see subchapter F (Marine Engineering) of...

  5. 46 CFR 189.25-35 - Marine engineering equipment.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 46 Shipping 7 2010-10-01 2010-10-01 false Marine engineering equipment. 189.25-35 Section 189.25... INSPECTION AND CERTIFICATION Inspection for Certification § 189.25-35 Marine engineering equipment. (a) For inspection procedures of Marine Engineering equipment and systems, see Subchapter F (Marine Engineering) of...

  6. 46 CFR 111.01-3 - Placement of equipment.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 46 Shipping 4 2012-10-01 2012-10-01 false Placement of equipment. 111.01-3 Section 111.01-3 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) ELECTRICAL ENGINEERING ELECTRIC SYSTEMS-GENERAL REQUIREMENTS General § 111.01-3 Placement of equipment. (a) Electric equipment must be arranged...

  7. 46 CFR 91.25-35 - Marine engineering equipment.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 46 Shipping 4 2012-10-01 2012-10-01 false Marine engineering equipment. 91.25-35 Section 91.25-35... INSPECTION AND CERTIFICATION Inspection for Certification § 91.25-35 Marine engineering equipment. (a) For inspection procedures of marine engineering equipment and systems, see subchapter F (Marine Engineering) of...

  8. Space station communications and tracking equipment management/control system

    NASA Technical Reports Server (NTRS)

    Kapell, M. H.; Seyl, J. W.

    1982-01-01

    Design details of a communications and tracking (C and T) local area network and the distribution system requirements for the prospective space station are described. The hardware will be constructed of LRUs, including those for baseband, RF, and antenna subsystems. It is noted that the C and T equipment must be routed throughout the station to accommodate growth of the station. Configurations of the C and T modules will therefore be dependent on the function of the space station module where they are located. A block diagram is provided of a sample C and T hardware distribution configuration. A topology and protocol will be needed to accommodate new terminals, wide bandwidths, bidirectional message transmission, and distributed functioning. Consideration will be given to collisions occurring in the data transmission channels.

  9. Research on speed control of secondary regulation lifting system of parking equipment

    NASA Astrophysics Data System (ADS)

    Zang, Faye

    2005-12-01

    Hydrostatic transmission with secondary regulation is a new kind of hydrostatic transmission that can regenerate inertial and gravitational energy of load. On the basis of an in-depth analysis of the working principles and energy-saving mechanisms of the parking equipment lifting systems with, secondary regulating technology, this paper proposes a method of regenerating a lifting system's inertial energy by controlling rotational speed and reclaiming the gravitational energy by use of a constant rotational speed. Considering large changes of the parameters of lifting systems and then non-linearity, a fuzzy control was adopted to control the lifting system, and a mathematical model of the system was established. By simulation and experiment of the lifting system, the conclusion was reached a lifting system's braking achieved by controlling rotational speed is reliable and stable at a definite speed. It also permits changing the efficiency of recovery by changing the rotational speed. The design power of the lifting system can be chosen in terms of the system's average power, so the system's power can be reduced and energy savings achieved.

  10. Selected retinoids: determination by isocratic normal-phase HPLC.

    PubMed

    Klvanova, J; Brtko, J

    2002-09-01

    Retinol (ROL), retinal (RAL) and retinoic acid (RA) are physiologically active forms of vitamin A. All-trans retinoic acid (ATRA) can be formed by oxidation from all-trans retinal (ATRAL). Isomerization of RA is considered to be an important metabolic pathway of retinoids. RA isomers transactivate various response pathways via their cognate nuclear receptors that act as ligand inducible transcription factors. The aim of this study was to establish a rapid and simple method for determination of ATRA, 13-cis retinoic acid (13CRA) and ATRAL by HPLC. In our laboratory, we slightly modified the method of Miyagi et al. (2001) and separated ATRA, 13CRA and ATRAL by simple isocratic normal phase HPLC. Both retinoic acid isomers and ATRAL were eluted within 13 min and all components were well resolved. The coefficients of variation (C.V.) for RAs and RAL were from 3.0 to 5.4 %.

  11. Development of a System to Validate Group 3 Facsimile Equipment. Phase I.

    DTIC Science & Technology

    1981-07-01

    such as modem , equalizer, line connection etc.) in hardware is. unavoidable. 3. Unless computer and test equipment are co-resident, hardware will be...network simulator. Most of this hardware/firmware has been developed for data transmission in general (v.27 ter/V.29 modems )or specifically for Group 3...system with the facsimile unit under test. 2. V.27 ter/V.29 modems - to handle facsimile data at the various data rate. 3. Modem control and switching

  12. Photooxidation of 3-substituted pyrroles:  a postcolumn reaction detection system for singlet molecular oxygen in HPLC.

    PubMed

    Denham, K; Milofsky, R E

    1998-10-01

    A postcolumn photochemical reaction detection scheme, based on the reaction of 3-substituted pyrroles with singlet molecular oxygen ((1)O(2)), has been developed. The method is selective and sensitive for the determination of a class of organic compounds called (1)O(2)-sensitizers and is readily coupled to HPLC. Following separation by HPLC, analytes ((1)O(2)-sensitizers) are excited by a Hg pen-ray lamp. Analytes that are efficient (1)O(2)-sensitizers promote ground-state O(2) ((3)Σ(g)(-)) to an excited state ((1)Σ(g)(+) or (1)Δ(g)), which reacts rapidly with tert-butyl-3,4,5-trimethylpyrrolecarboxylate (BTMPC) or N-benzyl-3-methoxypyrrole-2-tert-carboxylate (BMPC), which is added to the mobile phase. Detection is based on the loss of pyrrole (BTMPC or BMPC). The reaction is catalytic in nature since one analyte molecule may absorb light many times, producing large amounts of (1)O(2). Detection limits for several (1)O(2)-sensitizers were improved by 1-2 orders of magnitude over optimized UV-absorbance detection. This paper discusses the optimization of the reaction conditions for this photochemical reaction detection scheme and its application to the detection of PCBs, nitrogen heterocycles, nitro and chloro aromatics, and other substituted aromatic compounds.

  13. The use of capillary electrophoresis as part of a specificity testing strategy for mitoguazone dihydrochloride HPLC methods.

    PubMed

    Thomson, C E; Gray, M R; Baxter, M P

    1997-05-01

    Capillary electrophoresis (CE) has been used as part of a validation experiment designed to prove the specificity of high performance liquid chromatography (HPLC) methods used for analysis of mitoguazone dihydrochloride drug substance. Data regarding accuracy, precision and sensitivity of the CE methods are presented as well as a comparison of results obtained from CE, HPLC and thin-layer chromatography (TLC) analysis of samples stressed under a variety of conditions. It was concluded that, not only were the HPLC methods being investigated specific, but that CE could potentially be used to replace HPLC for the routine assay of mitoguazone dihydrochloride.

  14. [HPLC fingerprint chromatogram analysis of some Taraxacum in Henan province].

    PubMed

    Li, Xi-Feng; Shi, Hui-Min; Xu, Min; Meng, Lu

    2008-10-01

    To analyze the HPLC fingerprint chromatogram of some Taraxacum in Henan. Samples of different species, producing areas, harvest seasons and medicinal parts were determined by RP-HPLC. The chromatogram was evaluated by software of evaluating similarity. The components of different species in Taraxacum were the same and could be substituted for each other. The contents of coffeic acid and chlorogenic acid in different producing areas were very different,which in fecund soil was better. The period of flowering and fruiting in Spring was the best gather period, and the components in different parts were different. The quality of medicinal materal within Taraxacum should be controlled better by this method.

  15. Characterization of chemical constituents in Rhodiola Crenulate by high-performance liquid chromatography coupled with Fourier-transform ion cyclotron resonance mass spectrometer (HPLC-FT-ICR MS).

    PubMed

    Han, Fei; Li, Yanting; Mao, Xinjuan; Xu, Rui; Yin, Ran

    2016-05-01

    In this work, an approach using high-performance liquid chromatography coupled with diode-array detection and Fourier-transform ion cyclotron resonance mass spectrometer (HPLC-FT-ICR MS) for the identification and profiling of chemical constituents in Rhodiola crenulata was developed for the first time. The chromatographic separation was achieved on an Inertsil ODS-3 column (150 mm × 4.6 mm,3 µm) using a gradient elution program, and the detection was performed on a Bruker Solarix 7.0 T mass spectrometer equipped with electrospray ionization source in both positive and negative modes. Under the optimized conditions, a total of 48 chemical compounds, including 26 alcohols and their glycosides, 12 flavonoids and their glycosides, 5 flavanols and gallic acid derivatives, 4 organic acids and 1 cyanogenic glycoside were identified or tentatively characterized. The results indicated that the developed HPLC-FT-ICR MS method with ultra-high sensitivity and resolution is suitable for identifying and characterizing the chemical constituents in R. crenulata. And it provides a helpful chemical basis for further research on R. crenulata. Copyright © 2016 John Wiley & Sons, Ltd. Copyright © 2016 John Wiley & Sons, Ltd.

  16. Shuttle orbiter S-band communications equipment design evaluation

    NASA Technical Reports Server (NTRS)

    Springett, J. C.

    1979-01-01

    An assessment of S-band communication equipment includes: (1) the review and analysis of the ability of the various subsystem avionic equipment designs to interface with, and operate on signals from/to adjoining equipment; (2) the performance peculiarities of the hardware against the overall specified system requirements; and (3) the evaluation of EMC EMI test results of the various equipment with respect to the possibility of mutual interferences.

  17. Combined HPLC-CUPRAC (cupric ion reducing antioxidant capacity) assay of parsley, celery leaves, and nettle.

    PubMed

    Yildiz, Leyla; Başkan, Kevser Sözgen; Tütem, Esma; Apak, Reşat

    2008-10-19

    This study aims to identify the essential antioxidant compounds present in parsley (Petroselinum sativum) and celery (Apium graveolens) leaves belonging to the Umbelliferae (Apiaceae) family, and in stinging nettle (Urtica dioica) belonging to Urticaceae family, to measure the total antioxidant capacity (TAC) of these compounds with CUPRAC (cupric ion reducing antioxidant capacity) and ABTS spectrophotometric methods, and to correlate the TAC with high performance liquid chromatography (HPLC) findings. The CUPRAC spectrophotometric method of TAC assay using copper(II)-neocuproine (2,9-dimethyl-1,10-phenanthroline) as the chromogenic oxidant was developed in our laboratories. The individual antioxidant constituents of plant extracts were identified and quantified by HPLC on a C18 column using a modified mobile phase of gradient elution comprised of MeOH-0.2% o-phosphoric acid and UV detection for polyphenols at 280 nm. The TAC values of HPLC-quantified antioxidant constituents were found, and compared for the first time with those found by CUPRAC. The TAC of HPLC-quantified compounds accounted for a relatively high percentage of the observed CUPRAC capacities of plant extracts, namely 81% of nettle, 60-77% of parsley (in different hydrolyzates of extract and solid sample), and 41-57% of celery leaves (in different hydrolyzates). The CUPRAC total capacities of the 70% MeOH extracts of studied plants (in the units of mmol trolox g(-1)plant) were in the order: celery leaves>nettle>parsley. The TAC calculated with the aid of HPLC-spectrophotometry did not compensate for 100% of the CUPRAC total capacities, because all flavonoid glycosides subjected to hydrolysis were either not detectable with HPLC, or not converted to the corresponding aglycons (i.e., easily detectable and quantifiable with HPLC) during the hydrolysis step.

  18. Solar Equipment

    NASA Technical Reports Server (NTRS)

    1983-01-01

    A medical refrigeration and a water pump both powered by solar cells that convert sunlight directly into electricity are among the line of solar powered equipment manufactured by IUS (Independent Utility Systems) for use in areas where conventional power is not available. IUS benefited from NASA technology incorporated in the solar panel design and from assistance provided by Kerr Industrial Applications Center.

  19. 46 CFR 71.25-35 - Marine engineering equipment.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 46 Shipping 3 2013-10-01 2013-10-01 false Marine engineering equipment. 71.25-35 Section 71.25-35... CERTIFICATION Annual Inspection § 71.25-35 Marine engineering equipment. (a) For inspection procedures of marine engineering equipment and systems, see subchapter F. (Marine Engineering) of this chapter. (b) [Reserved] ...

  20. 46 CFR 71.25-35 - Marine engineering equipment.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 46 Shipping 3 2010-10-01 2010-10-01 false Marine engineering equipment. 71.25-35 Section 71.25-35... CERTIFICATION Annual Inspection § 71.25-35 Marine engineering equipment. (a) For inspection procedures of marine engineering equipment and systems, see subchapter F. (Marine Engineering) of this chapter. (b) [Reserved] ...

  1. 46 CFR 71.25-35 - Marine engineering equipment.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 46 Shipping 3 2014-10-01 2014-10-01 false Marine engineering equipment. 71.25-35 Section 71.25-35... CERTIFICATION Annual Inspection § 71.25-35 Marine engineering equipment. (a) For inspection procedures of marine engineering equipment and systems, see subchapter F. (Marine Engineering) of this chapter. (b) [Reserved] ...

  2. 46 CFR 71.25-35 - Marine engineering equipment.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 46 Shipping 3 2012-10-01 2012-10-01 false Marine engineering equipment. 71.25-35 Section 71.25-35... CERTIFICATION Annual Inspection § 71.25-35 Marine engineering equipment. (a) For inspection procedures of marine engineering equipment and systems, see subchapter F. (Marine Engineering) of this chapter. (b) [Reserved] ...

  3. 46 CFR 71.25-35 - Marine engineering equipment.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 46 Shipping 3 2011-10-01 2011-10-01 false Marine engineering equipment. 71.25-35 Section 71.25-35... CERTIFICATION Annual Inspection § 71.25-35 Marine engineering equipment. (a) For inspection procedures of marine engineering equipment and systems, see subchapter F. (Marine Engineering) of this chapter. (b) [Reserved] ...

  4. [HPLC specific chromatogram of Lamiophlomis Herba and its counterfeit and determination of four effective components].

    PubMed

    Zan, Ke; Jiao, Xing-Ping; Guo, Li-Nong; Zheng, Jian; Ma, Shuang-Cheng

    2016-06-01

    This study is to establish the HPLC specific chromatogram and determine four main effective components of Lamiophlomis Herba and its counterfeit.Chlorogenic acid, forsythoside B, acteoside and luteoloside were reference substance.HPLC analysis was performed on a Waters XSelect C₁₈ column (4.6 mm×250 mm,5 μm).The mobile phase was acetonitrile-0.5% phosphoric acid solution (18∶82) with isocratic elution.The flow rate was 1.0 mL•min⁻¹, the detection wavelength was 332 nm and the column temperature was 30 ℃.Chemometrics software Chempattern was employed to analyze the research data.HPLC specific chromatogram of Lamiophlomis Herba from different samples were of high similarity, but the similarity of the HPLC specific chromatogram of its counterfeit were less than 0.65.Both of cluster and principal component analysis can distinguish certified products and adulterants.The HPLC specific chromatogram and contents of four effective components can be used for the quality control of Lamiophlomis Herba and its preparations.It provided scientific basis to standardize the use of the crude drug. Copyright© by the Chinese Pharmaceutical Association.

  5. Targeting overall equipment efficiency for small medium enterprises with irregular production system

    NASA Astrophysics Data System (ADS)

    Prasetyawan, Y.; Suef, M.; Claudia, L.; Handayani, F. D.

    2018-04-01

    Overall Equipment Effectiveness (OEE) is widely used to measure the maturity of a production system. The company will be considered as World Class Manufacturing if it reaches more than 85% value, with near perfect value for availability, performance and quality factor. This assessment is usually taken on industries with regular production times named shift system. A typical 8 hours shift system is used in OEE measurement and performance monitoring. There are few Small to Medium Enterprise (SME) perform regular production times with shift systems, others using irregular production systems. The irregular production time in the SME production system is used because of demand fluctuations. This paper shows a quantitative analysis as a part of manufacturing system design to achieve a specific value of OEE for SME with irregular production systems, for individual businesses as well as collective business systems (some companies use the same production facilities for several processes). The results of experiments on several companies are presented, as a basis for determining the technical strategy of achieving OEE values.

  6. "Lull pgm System" for Autologous Fat Grafting: A Simple Closed System with Minimal Equipment and No Extra Cost.

    PubMed

    Morselli, Paolo Giovanni; Micai, Alessandro; Giorgini, Federico Armando

    2016-08-01

    The "Lull pgm system" is a closed system for purifying harvested fat. It processes the collected tissue safely without any additional cost. The system was conceived by referring to the targets described in the literature with the aim of creating a simple system that guarantees a high standard of purification and requires minimal equipment that is available in every operating room. Cost must be always considered: even the most prosperous hospitals must keep within tight annual budgets. "Lull" can be used instead of expensive devices or disposable kits, without substantially increasing the operating time. The system has been used in clinical practice for many plastic reconstructive procedures and has obtained positive results and patient satisfaction, and no contraindications or disadvantages have been observed.

  7. Determination of Biogenic Amines with HPLC-APCI-MS

    USDA-ARS?s Scientific Manuscript database

    Determination of biogenic amines in fish samples can be used as a quality attribute and are commonly performed using a derivatization step followed by high pressure liquid chromatography (HPLC) and UV detection. Over estimation and misidentification of biogenic amines can occur when interfering comp...

  8. Prioritizing equipment for replacement.

    PubMed

    Capuano, Mike

    2010-01-01

    It is suggested that clinical engineers take the lead in formulating evaluation processes to recommend equipment replacement. Their skill, knowledge, and experience, combined with access to equipment databases, make them a logical choice. Based on ideas from Fennigkoh's scheme, elements such as age, vendor support, accumulated maintenance cost, and function/risk were used.6 Other more subjective criteria such as cost benefits and efficacy of newer technology were not used. The element of downtime was also omitted due to the data element not being available. The resulting Periop Master Equipment List and its rationale was presented to the Perioperative Services Program Council. They deemed the criteria to be robust and provided overwhelming acceptance of the list. It was quickly put to use to estimate required capital funding, justify items already thought to need replacement, and identify high-priority ranked items for replacement. Incorporating prioritization criteria into an existing equipment database would be ideal. Some commercially available systems do have the basic elements of this. Maintaining replacement data can be labor-intensive regardless of the method used. There is usually little time to perform the tasks necessary for prioritizing equipment. However, where appropriate, a clinical engineering department might be able to conduct such an exercise as shown in the following case study.

  9. 47 CFR 76.1621 - Equipment compatibility offer.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 47 Telecommunication 4 2010-10-01 2010-10-01 false Equipment compatibility offer. 76.1621 Section 76.1621 Telecommunication FEDERAL COMMUNICATIONS COMMISSION (CONTINUED) BROADCAST RADIO SERVICES MULTICHANNEL VIDEO AND CABLE TELEVISION SERVICE Notices § 76.1621 Equipment compatibility offer. Cable system...

  10. 47 CFR 76.1621 - Equipment compatibility offer.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 47 Telecommunication 4 2013-10-01 2013-10-01 false Equipment compatibility offer. 76.1621 Section 76.1621 Telecommunication FEDERAL COMMUNICATIONS COMMISSION (CONTINUED) BROADCAST RADIO SERVICES MULTICHANNEL VIDEO AND CABLE TELEVISION SERVICE Notices § 76.1621 Equipment compatibility offer. Cable system...

  11. 47 CFR 76.1621 - Equipment compatibility offer.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 47 Telecommunication 4 2014-10-01 2014-10-01 false Equipment compatibility offer. 76.1621 Section 76.1621 Telecommunication FEDERAL COMMUNICATIONS COMMISSION (CONTINUED) BROADCAST RADIO SERVICES MULTICHANNEL VIDEO AND CABLE TELEVISION SERVICE Notices § 76.1621 Equipment compatibility offer. Cable system...

  12. 47 CFR 76.1621 - Equipment compatibility offer.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 47 Telecommunication 4 2011-10-01 2011-10-01 false Equipment compatibility offer. 76.1621 Section 76.1621 Telecommunication FEDERAL COMMUNICATIONS COMMISSION (CONTINUED) BROADCAST RADIO SERVICES MULTICHANNEL VIDEO AND CABLE TELEVISION SERVICE Notices § 76.1621 Equipment compatibility offer. Cable system...

  13. 47 CFR 76.1621 - Equipment compatibility offer.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 47 Telecommunication 4 2012-10-01 2012-10-01 false Equipment compatibility offer. 76.1621 Section 76.1621 Telecommunication FEDERAL COMMUNICATIONS COMMISSION (CONTINUED) BROADCAST RADIO SERVICES MULTICHANNEL VIDEO AND CABLE TELEVISION SERVICE Notices § 76.1621 Equipment compatibility offer. Cable system...

  14. Identification of phenolic compounds in red wine extract samples and zebrafish embryos by HPLC-ESI-LTQ-Orbitrap-MS.

    PubMed

    Vallverdú-Queralt, Anna; Boix, Nuria; Piqué, Ester; Gómez-Catalan, Jesús; Medina-Remon, Alexander; Sasot, Gemma; Mercader-Martí, Mercè; Llobet, Juan M; Lamuela-Raventos, Rosa M

    2015-08-15

    The zebrafish embryo is a highly interesting biological model with applications in different scientific fields, such as biomedicine, pharmacology and toxicology. In this study, we used liquid chromatography/electrospray ionisation-linear ion trap quadrupole-Orbitrap-mass spectrometry (HPLC/ESI-LTQ-Orbitrap-MS) to identify the polyphenol compounds in a red wine extract and zebrafish embryos. Phenolic compounds and anthocyanin metabolites were determined in zebrafish embryos previously exposed to the red wine extract. Compounds were identified by injection in a high-resolution system (LTQ-Orbitrap) using accurate mass measurements in MS, MS(2) and MS(3) modes. To our knowledge, this research constitutes the first comprehensive identification of phenolic compounds in zebrafish by HPLC coupled to high-resolution mass spectrometry. Copyright © 2015 Elsevier Ltd. All rights reserved.

  15. Automatic monitoring of vibration welding equipment

    DOEpatents

    Spicer, John Patrick; Chakraborty, Debejyo; Wincek, Michael Anthony; Wang, Hui; Abell, Jeffrey A; Bracey, Jennifer; Cai, Wayne W

    2014-10-14

    A vibration welding system includes vibration welding equipment having a welding horn and anvil, a host device, a check station, and a robot. The robot moves the horn and anvil via an arm to the check station. Sensors, e.g., temperature sensors, are positioned with respect to the welding equipment. Additional sensors are positioned with respect to the check station, including a pressure-sensitive array. The host device, which monitors a condition of the welding equipment, measures signals via the sensors positioned with respect to the welding equipment when the horn is actively forming a weld. The robot moves the horn and anvil to the check station, activates the check station sensors at the check station, and determines a condition of the welding equipment by processing the received signals. Acoustic, force, temperature, displacement, amplitude, and/or attitude/gyroscopic sensors may be used.

  16. Advanced technologies for maintenance of electrical systems and equipment at the Savannah River Site Defense Waste Processing Facility

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Husler, R.O.; Weir, T.J.

    1991-01-01

    An enhanced maintenance program is being established to characterize and monitor cables, components, and process response at the Savannah River Site, Defense Waste Processing Facility. This facility was designed and constructed to immobilize the radioactive waste currently stored in underground storage tanks and is expected to begin operation in 1993. The plant is initiating the program to baseline and monitor instrument and control (I C) and electrical equipment, remote process equipment, embedded instrument and control cables, and in-cell jumper cables used in the facility. This program is based on the electronic characterization and diagnostic (ECAD) system which was modified tomore » include process response analysis and to meet rigid Department of Energy equipment requirements. The system consists of computer-automated, state-of-the-art electronics. The data that are gathered are stored in a computerized database for analysis, trending, and troubleshooting. It is anticipated that the data which are gathered and trended will aid in life extension for the facility.« less

  17. Advanced technologies for maintenance of electrical systems and equipment at the Savannah River Site Defense Waste Processing Facility

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Husler, R.O.; Weir, T.J.

    1991-12-31

    An enhanced maintenance program is being established to characterize and monitor cables, components, and process response at the Savannah River Site, Defense Waste Processing Facility. This facility was designed and constructed to immobilize the radioactive waste currently stored in underground storage tanks and is expected to begin operation in 1993. The plant is initiating the program to baseline and monitor instrument and control (I&C) and electrical equipment, remote process equipment, embedded instrument and control cables, and in-cell jumper cables used in the facility. This program is based on the electronic characterization and diagnostic (ECAD) system which was modified to includemore » process response analysis and to meet rigid Department of Energy equipment requirements. The system consists of computer-automated, state-of-the-art electronics. The data that are gathered are stored in a computerized database for analysis, trending, and troubleshooting. It is anticipated that the data which are gathered and trended will aid in life extension for the facility.« less

  18. 40 CFR 60.254 - Standards for coal processing and conveying equipment, coal storage systems, transfer and loading...

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 40 Protection of Environment 6 2010-07-01 2010-07-01 false Standards for coal processing and conveying equipment, coal storage systems, transfer and loading systems, and open storage piles. 60.254... (CONTINUED) STANDARDS OF PERFORMANCE FOR NEW STATIONARY SOURCES Standards of Performance for Coal Preparation...

  19. Determination of trace amounts of rare-earth elements in highly pure neodymium oxide by sector field inductively coupled plasma mass spectrometry (ICP-SFMS) and high-performance liquid chromatography (HPLC) techniques

    NASA Astrophysics Data System (ADS)

    Pedreira, W. R.; Sarkis, J. E. S.; da Silva Queiroz, C. A.; Rodrigues, C.; Tomiyoshi, I. A.; Abrão, A.

    2003-02-01

    Recently rare-earth elements (REE) have received much attention in fields of geochemistry and industry. Rapid and accurate determinations of them are increasingly required as industrial demands expand. Sector field inductively coupled plasma mass spectrometry (ICP-SFMS) with high-performance liquid chromatography (HPLC) has been applied to the determination of REE. HR ICP-MS was used as an element-selective detector for HPLC in highly pure materials. The separation of REE with HPLC helped to avoid erroneous analytical results due to spectral interferences. Sixteen elements (Sc, Y and 14 lanthanides) were determined selectively with the HPLC/ICP-SFMS system using a concentration gradient methods. The detection limits with the HPLC/ICP-SFMS system were about 0.5-10 pg mL-1. The percentage recovery ranged from 90% to 100% for different REE. The %RSD of the methods varying between 2.5% and 4.5% for a set of five (n=5) replicates was found for the IPEN's material and for the certificate reference sample. Determination of trace REEs in two highly pure neodymium oxides samples (IPEN and Johnson Matthey Company) were performed. In short, the IPEN's materials which are highly pure (>99.9%) were successfully analyzed without spectral interferences.

  20. Ultrafast UPLC-ESI-MS and HPLC with monolithic column for determination of principal flavor compounds in vanilla pods.

    PubMed

    Sharma, Upendra K; Sharma, Nandini; Sinha, Arun K; Kumar, Neeraj; Gupta, Ajai P

    2009-10-01

    In this study, two novel chromatographic methods based on monolithic column high-performance liquid chromatography (HPLC) and ultra-performance liquid chromatography (UPLC) were developed for the ultrafast determination of principal flavor compounds namely vanillin, vanillic acid, p-hydroxybenzoic acid, and p-hydroxybenzaldehyde in ethanolic extracts of Vanilla planifolia pods. Good separation was achieved within 2.5 min using Chromolith RP18e column (100 mm x 4.6 mm) for HPLC and Acquity BEH C-18 (100 mm x 2.1 mm, 1.7 microm) column for UPLC. Both methods were compared in terms of total analysis time, mobile phase consumption, sensitivity, and validation parameters like precision, accuracy, LOD, and LOQ. Further, system suitability test data including resolution, capacity factor, theoretical plates, and tailing factor was determined for both the methods by ten replicate injections. Monolithic column based HPLC gave better results for most of the selected parameters while UPLC was found to be more eco-friendly with low mobile phase consumption and better sensitivity. Both methods may be used conveniently for the high throughput analysis of large number of samples in comparison to traditional particulate column.