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Sample records for human 3he lung

  1. A System for Open-Access 3He Human Lung Imaging at Very Low Field

    PubMed Central

    RUSET, I.C.; TSAI, L.L.; MAIR, R.W.; PATZ, S.; HROVAT, M.I.; ROSEN, M.S.; MURADIAN, I.; NG, J.; TOPULOS, G.P.; BUTLER, J.P.; WALSWORTH, R.L.; HERSMAN, F.W.

    2010-01-01

    We describe a prototype system built to allow open-access very-low-field MRI of human lungs using laser-polarized 3He gas. The system employs an open four-coil electromagnet with an operational B0 field of 4 mT, and planar gradient coils that generate gradient fields up to 0.18 G/cm in the x and y direction and 0.41 G/cm in the z direction. This system was used to obtain 1H and 3He phantom images and supine and upright 3He images of human lungs. We include discussion on challenges unique to imaging at 50 –200 kHz, including noise filtering and compensation for narrow-bandwidth coils. PMID:20354575

  2. Spectroscopic issues in optical polarization of 3He gas for Magnetic Resonance Imaging of human lungs

    NASA Astrophysics Data System (ADS)

    Dohnalik, T.; Głowacz, B.; Olejniczak, Z.; Pałasz, T.; Suchanek, M.; Wojna, A.

    2013-10-01

    The Magnetic Resonance Imaging (MRI) of human lungs for diagnostic purposes became possible by using nuclear spin hyperpolarized noble gases, such as 3He. One of the methods to polarize 3He is the Metastability Exchange Optical Pumping (MEOP), which up to now has been performed at low pressure of about 1 mbar and in low magnetic field below 0.1 T (standard conditions). The equilibrium nuclear polarization can reach up to 80%, but it is dramatically reduced during the subsequent gas compression to the atmospheric pressure that is necessary for the lungs examination. Further polarization losses occur during the transportation of the gas to the hospital scanner. It was shown recently that up to 50% polarization can be obtained at elevated pressure exceeding 20 mbar, by using magnetic field higher than 0.1 T (nonstandard conditions). Therefore, following the construction of the low-field MEOP polarizer located in the lab, a dedicated portable unit was developed, which uses the magnetic field of the 1.5 T MR medical scanner and works in the continuous-flow regime. The first in Poland MRI images of human lungs in vivo were obtained on the upgraded to 3He resonance frequency Siemens Sonata medical scanner. An evident improvement in the image quality was achieved when using the new technique. The paper shows how spectroscopic measurements of 3He carried out in various experimental conditions led both to useful practical results and to significant progress in understanding fundamental processes taking place during MEOP.

  3. Posture-Dependent Human 3He Lung Imaging in an Open Access MRI System: Initial Results

    PubMed Central

    Tsai, L. L.; Mair, R. W.; Li, C.-H.; Rosen, M. S.; Patz, S.; Walsworth, R. L.

    2008-01-01

    Rationale and Objectives The human lung and its functions are extremely sensitive to orientation and posture, and debate continues as to the role of gravity and the surrounding anatomy in determining lung function and heterogeneity of perfusion and ventilation. However, study of these effects is difficult. The conventional high-field magnets used for most hyperpolarized 3He MRI of the human lung, and most other common radiological imaging modalities including PET and CT, restrict subjects to lying horizontally, minimizing most gravitational effects. Materials and Methods In this paper, we briefly review the motivation for posture-dependent studies of human lung function, and present initial imaging results of human lungs in the supine and vertical body orientations using inhaled hyperpolarized 3He gas and an open-access MRI instrument. The open geometry of this MRI system features a “walk-in” capability that permits subjects to be imaged in vertical and horizontal positions, and potentially allows for complete rotation of the orientation of the imaging subject in a two-dimensional plane. Results Initial results include two-dimensional lung images acquired with ~ 4 × 8 mm in-plane resolution and three-dimensional images with ~ 2 cm slice thickness. Conclusion Effects of posture variation are observed, including posture-related effects of the diaphragm and distension of the lungs while vertical. PMID:18486009

  4. In vivo NMR of hyperpolarized 3He in the human lung at very low magnetic fields

    NASA Astrophysics Data System (ADS)

    Bidinosti, Christopher P.; Choukeife, Jamal; Nacher, Pierre-Jean; Tastevin, Geneviève

    2003-05-01

    We present NMR measurements of the diffusion of hyperpolarized 3He in the human lung performed at fields much lower than those of conventional MRI scanners. The measurements were made on standing subjects using homebuilt apparatus operating at 3 mT. O 2-limited transverse relaxation ( T2 up to 15-35 s) could be measured in vivo. Accurate global diffusion measurements have been performed in vivo and in a plastic bag; the average apparent diffusion coefficient (ADC) in vivo was 14.2±0.6 mm2/ s, whereas the diffusion coefficient in the bag ( 3He diluted in N 2) was 79.5±1 mm2/ s. 1D ADC mapping with high SNR (˜200-300) demonstrates the real possibility of performing quality lung imaging at extremely low fields.

  5. In vivo NMR of hyperpolarized 3He in the human lung at very low magnetic fields.

    PubMed

    Bidinosti, Christopher P; Choukeife, Jamal; Nacher, Pierre Jean; Tastevin, Geneviève

    2003-05-01

    We present NMR measurements of the diffusion of hyperpolarized 3He in the human lung performed at fields much lower than those of conventional MRI scanners. The measurements were made on standing subjects using homebuilt apparatus operating at 3mT. O(2)-limited transverse relaxation (T(2) up to 15-35s) could be measured in vivo. Accurate global diffusion measurements have been performed in vivo and in a plastic bag; the average apparent diffusion coefficient (ADC) in vivo was 14.2+/-0.6mm(2)/s, whereas the diffusion coefficient in the bag (3He diluted in N(2)) was 79.5+/-1mm(2)/s. 1D ADC mapping with high SNR ( approximately 200-300) demonstrates the real possibility of performing quality lung imaging at extremely low fields.

  6. An open-access, very-low-field MRI system for posture-dependent 3He human lung imaging

    NASA Astrophysics Data System (ADS)

    Tsai, L. L.; Mair, R. W.; Rosen, M. S.; Patz, S.; Walsworth, R. L.

    2008-08-01

    We describe the design and operation of an open-access, very-low-field, magnetic resonance imaging (MRI) system for in vivo hyperpolarized 3He imaging of the human lungs. This system permits the study of lung function in both horizontal and upright postures, a capability with important implications in pulmonary physiology and clinical medicine, including asthma and obesity. The imager uses a bi-planar B0 coil design that produces an optimized 65 G (6.5 mT) magnetic field for 3He MRI at 210 kHz. Three sets of bi-planar coils produce the x, y, and z magnetic field gradients while providing a 79-cm inter-coil gap for the imaging subject. We use solenoidal Q-spoiled RF coils for operation at low frequencies, and are able to exploit insignificant sample loading to allow for pre-tuning/matching schemes and for accurate pre-calibration of flip angles. We obtain sufficient SNR to acquire 2D 3He images with up to 2.8 mm resolution, and present initial 2D and 3D 3He images of human lungs in both supine and upright orientations. 1H MRI can also be performed for diagnostic and calibration reasons.

  7. An Open-Access, Very-Low-Field MRI System for Posture-Dependent 3He Human Lung Imaging

    PubMed Central

    Tsai, L. L.; Mair, R. W.; Rosen, M. S.; Patz, S.; Walsworth, R. L.

    2008-01-01

    We describe the design and operation of an open-access, very-low-field, magnetic resonance imaging (MRI) system for in-vivo hyperpolarized 3He imaging of the human lungs. This system permits the study of lung function in both horizontal and upright postures, a capability with important implications in pulmonary physiology and clinical medicine, including asthma and obesity. The imager uses a bi-planar B0 coil design that produces an optimized 65 G (6.5 mT) magnetic field for 3He MRI at 210 kHz. Three sets of bi-planar coils produce the x, y, and z magnetic field gradients while providing a 79-cm inter-coil gap for the imaging subject. We use solenoidal Q-spoiled RF coils for operation at low frequencies, and are able to exploit insignificant sample loading to allow for pre-tuning/matching schemes and for accurate pre-calibration of flip angles. We obtain sufficient SNR to acquire 2D 3He images with up to 2.8 mm resolution, and present initial 2D and 3D 3He images of human lungs in both supine and upright orientations. 1H MRI can also be performed for diagnostic and calibration reasons. PMID:18550402

  8. Demonstration of a compact compressor for application of metastability-exchange optical pumping of 3He to human lung imaging.

    PubMed

    Gentile, T R; Jones, G L; Thompson, A K; Rizi, R R; Roberts, D A; Dimitrov, I E; Reddy, R; Lipson, D A; Gefter, W; Schnall, M D; Leigh, J S

    2000-02-01

    Hyperpolarized gas magnetic resonance imaging has recently emerged as a method to image lungs, sinuses, and the brain. The best lung images to date have been produced using hyperpolarized 3He, which is produced by either spin-exchange or metastability-exchange optical pumping. For hyperpolarized gas MRI, the metastable method has demonstrated higher polarization levels and higher polarizing rates, but it requires compression of the hyperpolarized gas. Prior to this work, compression of hyperpolarized gas had only been accomplished using a large, complex and expensive apparatus. Here, human lung ventilation images are presented that were obtained using a compact compressor that is relatively simple and inexpensive. For this test, 1.1 bar-L of 15% hyperpolarized 3He gas was produced at the National Institute of Standards and Technology using a modified commercial diaphragm pump. The hyperpolarized gas was transported to the University of Pennsylvania in a holding field provided by a portable solenoid.

  9. Long-range diffusion of hyperpolarized 3He in explanted normal and emphysematous human lungs via magnetization tagging

    PubMed Central

    Woods, Jason C.; Yablonskiy, Dmitriy A.; Choong, Cliff K.; Chino, Kimiaki; Pierce, John A.; Hogg, James C.; Bentley, John; Cooper, Joel D.; Conradi, Mark S.; Macklem, Peter T.

    2007-01-01

    Long-range diffusivity of hyperpolarized 3He gas was measured from the decay rate of sinusoidally modulated longitudinal nuclear magnetization in three normal donor and nine severely emphysematous explanted human lungs. This (long-range) diffusivity, which we call Dsec, is measured over seconds and centimeters and is ~10 times smaller in healthy lungs (0.022 cm2/s) than the more traditionally measured Dmsec, which is measured over milliseconds and submillimeters. The increased restriction of Dsec reflects the complex, tortuous paths required to navigate long distances through the maze of branching peripheral airways. In emphysematous lungs, Dsec is substantially increased, with some regions showing nearly the unrestricted value of the self-diffusion coefficient (0.88 cm2/s for dilute 3He in air, a 40-fold increase). This suggests the presence of large collateral pathways opened by alveolar destruction that bypass the airways proper. This destruction was confirmed by comparison with histology in seven lungs and by removal of trapped gas via holes in the pleural surface in five lungs. PMID:16024528

  10. 3He Diffusion MRI of the Lung

    PubMed Central

    Conradi, Mark S.; Yablonskiy, Dmitriy A.; Woods, Jason C.; Gierada, David S.; Jacob, Richard E.; Chang, Yulin V.; Choong, Cliff K.; Sukstanskii, Alex L.; Tanoli, Tariq; Lefrak, Stephen S.; Cooper, Joel D.

    2007-01-01

    Rationale and Objectives MR imaging of the restricted diffusion of laser-polarized 3He gas provides unique insights into the changes in lung microstructure in emphysema. Results We discuss measurements of ventilation (spin density), mean diffusivity, and the anisotropy of diffusion, which yields the mean acinar airway radius. In addition, the use of spatially modulated longitudinal magnetization allows diffusion to be measured over longer distances and times, with sensitivity to collateral ventilation paths. Early results are also presented for spin density and diffusivity maps made with a perfluorinated inert gas, C3F8. Methods Techniques for purging and imaging excised lungs are discussed. PMID:16253852

  11. Recycling of 3He from lung magnetic resonance imaging.

    PubMed

    Salhi, Z; Grossmann, T; Gueldner, M; Heil, W; Karpuk, S; Otten, E W; Rudersdorf, D; Surkau, R; Wolf, U

    2012-06-01

    We have developed the means to recycle (3) He exhaled by patients after imaging the lungs using magnetic resonance of hyperpolarized (3) He. The exhaled gas is collected in a helium leak proof bag and further compressed into a steel bottle. The collected gas contains about 1-2% of (3) He, depending on the amount administered and the number of breaths collected to wash out the (3) He gas from the lungs. (3) He is separated from the exhaled air using zeolite molecular sieve adsorbent at 77 K followed by a cold head at 8 K. Residual gaseous impurities are finally absorbed by a commercial nonevaporative getter. The recycled (3) He gas features high purity, which is required for repolarization by metastability exchange optical pumping. At present, we achieve a collection efficiency of 80-84% for exhaled gas from healthy volunteers and cryogenic separation efficiency of 95%.

  12. A flexible 32-channel receive array combined with a homogeneous transmit coil for human lung imaging with hyperpolarized 3He at 1.5 T.

    PubMed

    Deppe, Martin H; Parra-Robles, Juan; Marshall, Helen; Lanz, Titus; Wild, Jim M

    2011-12-01

    Parallel imaging presents a promising approach for MRI of hyperpolarized nuclei, as the penalty in signal-to-noise ratio typically encountered with (1)H MRI due to a reduction in acquisition time can be offset by an increase in flip angle. The signal-to-noise ratio of hyperpolarized MRI generally exhibits a strong dependence on flip angle, which makes a homogeneous B(1)(+) transmit field desirable. This paper presents a flexible 32-channel receive array for (3) He human lung imaging at 1.5T designed for insertion into an asymmetric birdcage transmit coil. While the 32-channel array allows parallel imaging at high acceleration factors, the birdcage transmit coil provides a homogeneous B(1)(+) field. Decoupling between array elements is achieved by using a concentric shielding approach together with preamplifier decoupling. Coupling between transmit coil and array elements is low by virtue of a low geometric coupling coefficient, which is reduced further by the concentric shields in the array. The combination of the 32-channel array and birdcage transmit coil provides (3)He ventilation images of excellent quality with similar signal-to-noise ratio at acceleration factors R = 2 and R = 4, while maintaining a homogeneous B(1)(+). Copyright © 2011 Wiley Periodicals, Inc.

  13. Comparison between 2D and 3D gradient-echo sequences for MRI of human lung ventilation with hyperpolarized 3He.

    PubMed

    Wild, Jim M; Woodhouse, Neil; Paley, Martyn N J; Fichele, Stan; Said, Zead; Kasuboski, Larry; van Beek, Edwin J R

    2004-09-01

    Images of hyperpolarized 3He were acquired during breath-hold in four healthy volunteers with the use of an optimized 3D gradient-echo sequence. The images were compared with existing 2D gradient-echo methods. The average SNR from a 13-mm-thick slice in the peripheral lung was 1.4 times greater with 3D. In the airways the average SNR was 1.7 times greater with 3D. The higher SNR of 3D was particularly evident when regions of unimpeded gas diffusion, such as the major airways, were imaged with thin slices. This is because diffusion dephasing due to the slice-encoding gradient is minimized with a 3D sequence. The in vivo experimental findings were substantiated with experiments on phantoms of free gas, which showed more than four times the SNR with 3D compared to 2D. Theoretical simulations of the 2D and 3D k-space filters were also performed to predict the SNR and spatial resolution observed in the experimental images. Copyright 2004 Wiley-Liss, Inc.

  14. In vivo lung morphometry with hyperpolarized 3He diffusion MRI: Theoretical background

    NASA Astrophysics Data System (ADS)

    Sukstanskii, A. L.; Yablonskiy, D. A.

    2008-02-01

    MRI-based study of 3He gas diffusion in lungs may provide important information on lung microstructure. Lung acinar airways can be described in terms of cylinders covered with alveolar sleeve [Haefeli-Bleuer, Weibel, Anat. Rec. 220 (1988) 401]. For relatively short diffusion times (on the order of a few ms) this geometry allows description of the 3He diffusion attenuated MR signal in lungs in terms of two diffusion coefficients—longitudinal (D) and transverse (D) with respect to the individual acinar airway axis [Yablonskiy et al., PNAS 99 (2002) 3111]. In this paper, empirical relationships between D and D and the geometrical parameters of airways and alveoli are found by means of computer Monte Carlo simulations. The effects of non-Gaussian signal behavior (dependence of D and D on b-value) are also taken into account. The results obtained are quantitatively valid in the physiologically important range of airway parameters characteristic of healthy lungs and lungs with mild emphysema. In lungs with advanced emphysema, the results provide only "apparent" characteristics but still could potentially be used to evaluate emphysema progression. This creates a basis for in vivo lung morphometry—evaluation of the geometrical parameters of acinar airways from hyperpolarized 3He diffusion MRI, despite the airways being too small to be resolved by direct imaging. These results also predict a rather substantial dependence of 3He ADC on the experimentally-controllable diffusion time, Δ. If Δ is decreased from 3 ms to 1 ms, the ADC in normal human lungs may increase by almost 50%. This effect should be taken into account when comparing experimental data obtained with different pulse sequences.

  15. In vivo lung morphometry with hyperpolarized 3He diffusion MRI: theoretical background.

    PubMed

    Sukstanskii, A L; Yablonskiy, D A

    2008-02-01

    MRI-based study of (3)He gas diffusion in lungs may provide important information on lung microstructure. Lung acinar airways can be described in terms of cylinders covered with alveolar sleeve [Haefeli-Bleuer, Weibel, Anat. Rec. 220 (1988) 401]. For relatively short diffusion times (on the order of a few ms) this geometry allows description of the (3)He diffusion attenuated MR signal in lungs in terms of two diffusion coefficients-longitudinal (D(L)) and transverse (D(T)) with respect to the individual acinar airway axis [Yablonskiy et al., PNAS 99 (2002) 3111]. In this paper, empirical relationships between D(L) and D(T) and the geometrical parameters of airways and alveoli are found by means of computer Monte Carlo simulations. The effects of non-Gaussian signal behavior (dependence of D(L) and D(T) on b-value) are also taken into account. The results obtained are quantitatively valid in the physiologically important range of airway parameters characteristic of healthy lungs and lungs with mild emphysema. In lungs with advanced emphysema, the results provide only "apparent" characteristics but still could potentially be used to evaluate emphysema progression. This creates a basis for in vivo lung morphometry-evaluation of the geometrical parameters of acinar airways from hyperpolarized (3)He diffusion MRI, despite the airways being too small to be resolved by direct imaging. These results also predict a rather substantial dependence of (3)He ADC on the experimentally-controllable diffusion time, Delta. If Delta is decreased from 3 ms to 1 ms, the ADC in normal human lungs may increase by almost 50%. This effect should be taken into account when comparing experimental data obtained with different pulse sequences.

  16. Distal airways in humans: dynamic hyperpolarized 3He MR imaging--feasibility

    NASA Technical Reports Server (NTRS)

    Tooker, Angela C.; Hong, Kwan Soo; McKinstry, Erin L.; Costello, Philip; Jolesz, Ferenc A.; Albert, Mitchell S.

    2003-01-01

    Dynamic hyperpolarized helium 3 (3He) magnetic resonance (MR) imaging of the human airways is achieved by using a fast gradient-echo pulse sequence during inhalation. The resulting dynamic images show differential contrast enhancement of both distal airways and the lung periphery, unlike static hyperpolarized 3He MR images on which only the lung periphery is seen. With this technique, up to seventh-generation airway branching can be visualized. Copyright RSNA, 2003.

  17. Distal airways in humans: dynamic hyperpolarized 3He MR imaging--feasibility

    NASA Technical Reports Server (NTRS)

    Tooker, Angela C.; Hong, Kwan Soo; McKinstry, Erin L.; Costello, Philip; Jolesz, Ferenc A.; Albert, Mitchell S.

    2003-01-01

    Dynamic hyperpolarized helium 3 (3He) magnetic resonance (MR) imaging of the human airways is achieved by using a fast gradient-echo pulse sequence during inhalation. The resulting dynamic images show differential contrast enhancement of both distal airways and the lung periphery, unlike static hyperpolarized 3He MR images on which only the lung periphery is seen. With this technique, up to seventh-generation airway branching can be visualized. Copyright RSNA, 2003.

  18. Ventilation-based segmentation of the lungs using hyperpolarized (3)He MRI.

    PubMed

    Tustison, Nicholas J; Avants, Brian B; Flors, Lucia; Altes, Talissa A; de Lange, Eduard E; Mugler, John P; Gee, James C

    2011-10-01

    To develop an automated segmentation method to differentiate the ventilated lung volume on (3) He magnetic resonance imaging (MRI). Computational processing (CP) for each subject consisted of the following three essential steps: 1) inhomogeneity bias correction, 2) whole lung segmentation, and 3) subdivision of the lung segmentation into regions of similar ventilation. Evaluation consisted of two comparative analyses: i) comparison of the number of defects scored by two human readers in 43 subjects, and ii) simultaneous truth and performance level estimation (STAPLE) in 18 subjects in which the ventilation defects were manually segmented by four human readers. There was excellent correlation between the number of ventilation defects tabulated by CP and reader #1 (intraclass correlation coefficient [ICC] = 0.86), CP and reader #2 (ICC = 0.85), and between the two readers (ICC = 0.97). The STAPLE results from the second analysis yielded the following sensitivity/specificity numbers: CP (0.898/0.905), radiologist #1 (0.743/0.897), radiologist #2 (0.501/0.985), radiologist #3 (0.898/0.848), and the first author (0.600/0.984). We developed and evaluated an automated method for quantifying the ventilated lung volume on (3) He MRI. The findings strongly indicate that our proposed algorithmic processing may be a reliable, automatic method for quantitating ventilation defects. Copyright © 2011 Wiley-Liss, Inc.

  19. (3)He-MRI in follow-up of lung transplant recipients.

    PubMed

    Gast, Klaus Kurt; Zaporozhan, Julia; Ley, Sebastian; Biedermann, Alexander; Knitz, Frank; Eberle, Balthasar; Schmiedeskamp, Joerg; Heussel, Claus-Peter; Mayer, Eckhard; Schreiber, Wolfgang Günter; Thelen, Manfred; Kauczor, Hans-Ulrich

    2004-01-01

    The aim of this study was to evaluate the possible contribution of (3)He-MRI to detect obliterative bronchiolitis (OB) in the follow-up of lung transplant recipients. Nine single- and double-lung transplanted patients were studied by an initial and a follow-up (3)He-MRI study. Images were evaluated subjectively by estimation of ventilation defect area and quantitatively by individually adapted threshold segmentation and subsequent calculation of ventilated lung volume. Bronchiolitis obliterans syndrome (BOS) was diagnosed using pulmonary function tests. At (3)He-MRI, OB was suspected if ventilated lung volume had decreased by 10% or more at the follow-up MRI study compared with the initial study. General accordance between pulmonary function testing and (3)He-MRI was good, although subjective evaluation of (3)He-MRI underestimated improvement in ventilation as obtained by pulmonary function tests. The (3)He-MRI indicated OB in 6 cases. According to pulmonary function tests, BOS was diagnosed in 5 cases. All diagnoses of BOS were also detected by (3)He-MRI. In 2 of these 5 cases, (3)He-MRI indicated OB earlier than pulmonary function tests. The results support the hypothesis that (3)He-MRI may be sensitive for early detection of OB and emphasize the need for larger prospective follow-up studies.

  20. In Vivo Lung Morphometry with Accelerated Hyperpolarized 3He Diffusion MRI: A Preliminary Study

    PubMed Central

    Chang, Yulin V.; Quirk, James D.; Yablonskiy, Dmitriy A.

    2014-01-01

    Purpose Parallel imaging can be used to reduce imaging time and to increase the spatial coverage in hyperpolarized gas MRI of the lung. In this proof-of-concept study we investigate the effects of parallel imaging on the morphometric measurement of lung microstructure using diffusion MRI with hyperpolarized 3He. Methods Fully sampled and under-sampled multi-b diffusion data were acquired from human subjects using an 8-channel 3He receive coil. A parallel imaging reconstruction technique (GRAPPA) was used to reconstruct under-sampled k-space data. The morphometric results of the GRAPPA-reconstructed data were compared with the results of fully sampled data for three types of subjects: healthy volunteers, mild, and moderate COPD patients. Results Morphometric measurements varied only slightly at mild acceleration factors. The results were largely well preserved compared to fully sampled data for different lung conditions. Conclusion Parallel imaging, given sufficient signal-to-noise ratio, provides a reliable means to accelerate hyperpolarized-gas MRI with no significant difference in the measurement of lung morphometry from the fully sampled images. GRAPPA is a promising technique to significantly reduce imaging time and/or to improve the spatial coverage for the morphometric measurement with hyperpolarized gases. PMID:24799044

  1. Quantitative in vivo assessment of lung microstructure at the alveolar level with hyperpolarized 3He diffusion MRI

    NASA Astrophysics Data System (ADS)

    Yablonskiy, Dmitriy A.; Sukstanskii, Alexander L.; Leawoods, Jason C.; Gierada, David S.; Bretthorst, G. Larry; Lefrak, Stephen S.; Cooper, Joel D.; Conradi, Mark S.

    2002-03-01

    The study of lung emphysema dates back to the beginning of the 17th century. Nevertheless, a number of important questions remain unanswered because a quantitative localized characterization of emphysema requires knowledge of lung structure at the alveolar level in the intact living lung. This information is not available from traditional imaging modalities and pulmonary function tests. Herein, we report the first in vivo measurements of lung geometrical parameters at the alveolar level obtained with 3He diffusion MRI in healthy human subjects and patients with severe emphysema. We also provide the first experimental data demonstrating that 3He gas diffusivity in the acinus of human lung is highly anisotropic. A theory of anisotropic diffusion is presented. Our results clearly demonstrate substantial differences between healthy and emphysematous lung at the acinar level and may provide new insights into emphysema progression. The technique offers promise as a clinical tool for early diagnosis of emphysema.

  2. 3D MRI of non-Gaussian (3)He gas diffusion in the rat lung.

    PubMed

    Jacob, Richard E; Laicher, Gernot; Minard, Kevin R

    2007-10-01

    In (3)He magnetic resonance images of pulmonary air spaces, the confining architecture of the parenchymal tissue results in a non-Gaussian distribution of signal phase that non-exponentially attenuates image intensity as diffusion weighting is increased. Here, two approaches previously used for the analysis of non-Gaussian effects in the lung are compared and related using diffusion-weighted (3)He MR images of mechanically ventilated rats. One approach is model-based and was presented by Yablonskiy et al., while the other approach utilizes the second order decay contribution that is predicted from the cumulant expansion theorem. Total lung coverage is achieved using a hybrid 3D pulse sequence that combines conventional phase encoding with sparse radial sampling for efficient gas usage. This enables the acquisition of nine 3D images using a total of only approximately 1 L of hyperpolarized (3)He gas. Diffusion weighting ranges from 0 s/cm(2) to 40 s/cm(2). Results show that the non-Gaussian effects of (3)He gas diffusion in healthy rat lungs are directly attributed to the anisotropic geometry of lung microstructure as predicted by the Yablonskiy model, and that quantitative analysis over the entire lung can be reliably repeated in time-course studies of the same animal.

  3. 3D MRI of non-Gaussian 3He gas diffusion in the rat lung

    NASA Astrophysics Data System (ADS)

    Jacob, Richard E.; Laicher, Gernot; Minard, Kevin R.

    2007-10-01

    In 3He magnetic resonance images of pulmonary air spaces, the confining architecture of the parenchymal tissue results in a non-Gaussian distribution of signal phase that non-exponentially attenuates image intensity as diffusion weighting is increased. Here, two approaches previously used for the analysis of non-Gaussian effects in the lung are compared and related using diffusion-weighted 3He MR images of mechanically ventilated rats. One approach is model-based and was presented by Yablonskiy et al., while the other approach utilizes the second order decay contribution that is predicted from the cumulant expansion theorem. Total lung coverage is achieved using a hybrid 3D pulse sequence that combines conventional phase encoding with sparse radial sampling for efficient gas usage. This enables the acquisition of nine 3D images using a total of only ˜1 L of hyperpolarized 3He gas. Diffusion weighting ranges from 0 s/cm 2 to 40 s/cm 2. Results show that the non-Gaussian effects of 3He gas diffusion in healthy rat lungs are directly attributed to the anisotropic geometry of lung microstructure as predicted by the Yablonskiy model, and that quantitative analysis over the entire lung can be reliably repeated in time-course studies of the same animal.

  4. Quantitative assessment of lung microstructure in healthy mice using an MR-based 3He lung morphometry technique

    PubMed Central

    Osmanagic, E.; Sukstanskii, A. L.; Quirk, J. D.; Woods, J. C.; Pierce, R. A.; Conradi, M. S.; Weibel, E. R.

    2010-01-01

    The recently developed technique of lung morphometry using hyperpolarized 3He diffusion magnetic resonance (MR) (Yablonskiy DA, Sukstanskii AL, Woods JC, Gierada DS, Quirk JD, Hogg JC, Cooper JD, Conradi MS. J Appl Physiol 107: 1258–1265, 2009) permits in vivo study of lung microstructure at the alveolar level. Originally proposed for human lungs, it also has the potential to study small animals. The technique relies on theoretical developments in the area of gas diffusion in lungs linking the diffusion attenuated MR signal to the lung microstructure. To adapt this technique to small animals, certain modifications in MR protocol and data analysis are required, reflecting the smaller size of mouse alveoli and acinar airways. This is the subject of the present paper. Herein, we established empirical relationships relating diffusion measurements to geometrical parameters of lung acinar airways with dimensions typical for mice and rats by using simulations of diffusion in the airways. We have also adjusted the MR protocol to acquire data with much shorter diffusion times compared with humans to accommodate the substantially smaller acinar airway length. We apply this technique to study mouse lungs ex vivo. Our MR-based measurements yield mean values of lung surface-to-volume ratio of 670 cm−1, alveolar density of 3,200 per mm3, alveolar depth of 55 μm, and mean chord length of 62 μm, all consistent with published data obtained histologically in mice by unbiased methods. The proposed technique can be used for in vivo experiments, opening a door for longitudinal studies of lung morphometry in mice and other small animals. PMID:20798272

  5. Dynamic ventilation 3He MRI for the quantification of disease in the rat lung.

    PubMed

    Kyriazis, Angelos; Rodriguez, I; Nin, N; Izquierdo-Garcia, J L; Lorente, J A; Perez-Sanchez, J M; Pesic, J; Olsson, L E; Ruiz-Cabello, J

    2012-03-01

    Pulmonary diseases are known to be largely inhomogeneous. To evaluate such inhomogeneities, we are testing an image-based method to measure gas flow in the lung regionally. Dynamic, spin-density-weighted hyperpolarized (3)He MR images performed during slow inhalation of this gas were analyzed to quantify regional inflation rate. This parameter was measured in regions of interest (ROIs) that were defined by a rectangular grid that covered the entire rat lung and grew dynamically with it during its inflation. We used regional inflation rate to quantify elastase-induced emphysema and to differentiate healthy (n = 8) from elastase-treated (n = 9) rat lungs as well as healthy from elastase-treated areas of one rat unilaterally treated with elastase in the left lung. Emphysema was also assessed by gold standard morphological and well-established hyperpolarized (3)He MRI diffusion measurements. Mean values of regional inflation rates were significantly different for healthy and elastase-treated animals and correlated well with the apparent diffusion coefficient of (3)He and morphological measurements. The image-based biomarker inflation rate may be useful for the assessment of regional lung ventilation.

  6. Linking Ventilation Heterogeneity Quantified via Hyperpolarized 3He MRI to Dynamic Lung Mechanics and Airway Hyperresponsiveness.

    PubMed

    Lui, Justin K; Parameswaran, Harikrishnan; Albert, Mitchell S; Lutchen, Kenneth R

    2015-01-01

    Advancements in hyperpolarized helium-3 MRI (HP 3He-MRI) have introduced the ability to render and quantify ventilation patterns throughout the anatomic regions of the lung. The goal of this study was to establish how ventilation heterogeneity relates to the dynamic changes in mechanical lung function and airway hyperresponsiveness in asthmatic subjects. In four healthy and nine mild-to-moderate asthmatic subjects, we measured dynamic lung resistance and lung elastance from 0.1 to 8 Hz via a broadband ventilation waveform technique. We quantified ventilation heterogeneity using a recently developed coefficient of variation method from HP 3He-MRI imaging. Dynamic lung mechanics and imaging were performed at baseline, post-challenge, and after a series of five deep inspirations. AHR was measured via the concentration of agonist that elicits a 20% decrease in the subject's forced expiratory volume in one second compared to baseline (PC20) dose. The ventilation coefficient of variation was correlated to low-frequency lung resistance (R = 0.647, P < 0.0001), the difference between high and low frequency lung resistance (R = 0.668, P < 0.0001), and low-frequency lung elastance (R = 0.547, P = 0.0003). In asthmatic subjects with PC20 values <25 mg/mL, the coefficient of variation at baseline exhibited a strong negative trend (R = -0.798, P = 0.02) to PC20 dose. Our findings were consistent with the notion of peripheral rather than central involvement of ventilation heterogeneity. Also, the degree of AHR appears to be dependent on the degree to which baseline airway constriction creates baseline ventilation heterogeneity. HP 3He-MRI imaging may be a powerful predictor of the degree of AHR and in tracking the efficacy of therapy.

  7. Linking Ventilation Heterogeneity Quantified via Hyperpolarized 3He MRI to Dynamic Lung Mechanics and Airway Hyperresponsiveness

    PubMed Central

    Lui, Justin K.; Parameswaran, Harikrishnan; Albert, Mitchell S.; Lutchen, Kenneth R.

    2015-01-01

    Advancements in hyperpolarized helium-3 MRI (HP 3He-MRI) have introduced the ability to render and quantify ventilation patterns throughout the anatomic regions of the lung. The goal of this study was to establish how ventilation heterogeneity relates to the dynamic changes in mechanical lung function and airway hyperresponsiveness in asthmatic subjects. In four healthy and nine mild-to-moderate asthmatic subjects, we measured dynamic lung resistance and lung elastance from 0.1 to 8 Hz via a broadband ventilation waveform technique. We quantified ventilation heterogeneity using a recently developed coefficient of variation method from HP 3He-MRI imaging. Dynamic lung mechanics and imaging were performed at baseline, post-challenge, and after a series of five deep inspirations. AHR was measured via the concentration of agonist that elicits a 20% decrease in the subject’s forced expiratory volume in one second compared to baseline (PC20) dose. The ventilation coefficient of variation was correlated to low-frequency lung resistance (R = 0.647, P < 0.0001), the difference between high and low frequency lung resistance (R = 0.668, P < 0.0001), and low-frequency lung elastance (R = 0.547, P = 0.0003). In asthmatic subjects with PC20 values <25 mg/mL, the coefficient of variation at baseline exhibited a strong negative trend (R = -0.798, P = 0.02) to PC20 dose. Our findings were consistent with the notion of peripheral rather than central involvement of ventilation heterogeneity. Also, the degree of AHR appears to be dependent on the degree to which baseline airway constriction creates baseline ventilation heterogeneity. HP 3He-MRI imaging may be a powerful predictor of the degree of AHR and in tracking the efficacy of therapy. PMID:26569412

  8. Emphysema Quantification in Inflation-Fixed Lungs Using Low-Dose Computed Tomography and 3He Magnetic Resonance Imaging

    SciTech Connect

    Gierada, David S.; Woods, Jason C.; Jacob, Rick E.; Bierhals, Andrew J.; Choong, Cliff K.; Bartel, Seth T.; Chang, Yulin V.; Das, Nitin A.; Hong, Cheng; Lutey, Barbara; Ritter, Jon H.; Pilgram, Thomas K.; Cooper, Joel D.; Patterson, G Alexander; Battafarano, Richard J.; Meyers, Bryan F.; Yablonskiy, Dmitriy A.; Conradi, Mark S.

    2010-09-02

    Abstract: Objective: To evaluate the use of inflation-fixed lung tissue for emphysema quantification with CT and 3He MR diffusion imaging. Methods: Fourteen subjects representing a range of chronic obstructive pulmonary disease severity who underwent complete or lobar lung resection were studied. CT measurements of lung attenuation and MR measurements of the hyperpolarized 3He apparent diffusion coefficient (ADC) in resected specimens fixed in inflation with heated formalin vapor were compared with measurements obtained before fixation. Results: The mean CT emphysema index was 56% ± 17% before and 58% ± 19% after fixation (P=0.77;R=0.76). Index differences correlated with differences in lung volume (R2=0.47). The mean 3He ADC was 0.40 ± 0.15 cm2/sec before and 0.39 ± 0.14 cm2/sec after fixation (P=0.03, R=0.98). The CT emphysema index and the 3He ADC were correlated before (R=0.89) and after fixation (R=0.79). Conclusion: Concordance of CT and 3He MR imaging measurements in unfixed and inflation-fixed lungs supports the use of inflation-fixed lungs for quantitative imaging studies in emphysema.

  9. A hybrid multibreath wash-in wash-out lung function quantification scheme in human subjects using hyperpolarized (3) He MRI for simultaneous assessment of specific ventilation, alveolar oxygen tension, oxygen uptake, and air trapping.

    PubMed

    Hamedani, Hooman; Kadlecek, Stephen; Xin, Yi; Siddiqui, Sarmad; Gatens, Heather; Naji, Joseph; Ishii, Masaru; Cereda, Maurizio; Rossman, Milton; Rizi, Rahim

    2017-08-01

    To present a method for simultaneous acquisition of alveolar oxygen tension (PA O2 ), specific ventilation (SV), and apparent diffusion coefficient (ADC) of hyperpolarized (HP) gas in the human lung, allowing reinterpretation of the PA O2 and SV maps to produce a map of oxygen uptake (R). An imaging scheme was designed with a series of identical normoxic HP gas wash-in breaths to measure ADC, SV, PA O2 , and R in less than 2 min. Signal dynamics were fit to an iterative recursive model that regionally solved for these parameters. This measurement was successfully performed in 12 subjects classified in three healthy, smoker, and chronic obstructive pulmonary disease (COPD) cohorts. The overall whole lung ADC, SV, PA O2 , and R in healthy, smoker, and COPD subjects was 0.20 ± 0.03 cm(2) /s, 0.39 ± 0.06,113 ± 2 Torr, and 1.55 ± 0.35 Torr/s, respectively, in healthy subjects; 0.21 ± 0.03 cm(2) /s, 0.33 ± 0.06, 115.9 ± 4 Torr, and 0.97 ± 0.2 Torr/s, respectively, in smokers; and 0.25 ± 0.06 cm(2) /s, 0.23 ± 0.08, 114.8 ± 6.0Torr, and 0.94 ± 0.12 Torr/s, respectively, in subjects with COPD. Hetrogeneity of SV, PA O2 , and R were indicators of both smoking-related changes and disease, and the severity of the disease correlated with the degree of this heterogeneity. Subjects with symptoms showed reduced oxygen uptake and specific ventilation. High-resolution, nearly coregistered and quantitative measures of lung function and structure were obtained with less than 1 L of HP gas. This hybrid multibreath technique produced measures of lung function that revealed clear differences among the cohorts and subjects and were confirmed by correlations with global lung measurements. Magn Reson Med 78:611-624, 2017. © 2016 International Society for Magnetic Resonance in Medicine. © 2016 International Society for Magnetic Resonance in Medicine.

  10. Hyperpolarized (3)He magnetic resonance imaging: comparison with four-dimensional x-ray computed tomography imaging in lung cancer.

    PubMed

    Mathew, Lindsay; Wheatley, Andrew; Castillo, Richard; Castillo, Edward; Rodrigues, George; Guerrero, Thomas; Parraga, Grace

    2012-12-01

    Pulmonary functional imaging using four-dimensional x-ray computed tomographic (4DCT) imaging and hyperpolarized (3)He magnetic resonance imaging (MRI) provides regional lung function estimates in patients with lung cancer in whom pulmonary function measurements are typically dominated by tumor burden. The aim of this study was to evaluate the quantitative spatial relationship between 4DCT and hyperpolarized (3)He MRI ventilation maps. Eleven patients with lung cancer provided written informed consent to 4DCT imaging and MRI performed within 11 ± 14 days. Hyperpolarized (3)He MRI was acquired in breath-hold after inhalation from functional residual capacity of 1 L hyperpolarized (3)He, whereas 4DCT imaging was acquired over a single tidal breath of room air. For hyperpolarized (3)He MRI, the percentage ventilated volume was generated using semiautomated segmentation; for 4DCT imaging, pulmonary function maps were generated using the correspondence between identical tissue elements at inspiratory and expiratory phases to generate percentage ventilated volume. After accounting for differences in image acquisition lung volumes ((3)He MRI: 1.9 ± 0.5 L ipsilateral, 2.3 ± 0.7 L contralateral; 4DCT imaging: 1.2 ± 0.3 L ipsilateral, 1.3 ± 0.4 L contralateral), there was no significant difference in percentage ventilated volume between hyperpolarized (3)He MRI (72 ± 11% ipsilateral, 79 ± 12% contralateral) and 4DCT imaging (74 ± 3% ipsilateral, 75 ± 4% contralateral). Spatial correspondence between 4DCT and (3)He MRI ventilation was evaluated using the Dice similarity coefficient index (ipsilateral, 86 ± 12%; contralateral, 88 ± 12%). Despite rather large differences in image acquisition breathing maneuvers, good spatial and significant quantitative agreement was observed for ventilation maps on hyperpolarized (3)He MRI and 4DCT imaging, suggesting that pulmonary regions with good lung function are similar between modalities in this small group of patients with

  11. Hyperpolarized 3He MR imaging of the lung: effect of subject immobilization on the occurrence of ventilation defects.

    PubMed

    Mata, Jaime; Altes, Talissa; Knake, Jeffrey; Mugler, John; Brookeman, James; de Lange, Eduard

    2008-02-01

    To investigate immobilization-induced ventilation defects when performing hyperpolarized (3)He (H(3)He) magnetic resonance imaging (MRI) of the lung. Twelve healthy subjects underwent MRI of the lungs after inhalation of H(3)He gas at three time points: 1) immediately after having been positioned supine on the MRI scanner table, 2) at 45 minutes while remaining supine, 3) and immediately thereafter after having turned prone. All image sets were reviewed in random order by three independent, blinded readers who recorded number, location, and size of H(3)He ventilation defects. Scores were averaged for each time point and comparisons were made to determine change in number, location, and size of ventilation defects with time and positioning of the subject in the scanner. At baseline supine, there were small numbers of defects in the dependent (posterior) and nondependent (anterior) portions of the lung (P = .625). At 45 minutes, there was a significant increase in the mean number of ventilation defects/slice (VDS) for the dependent (P = .005) and a decrease for the nondependent lung portions (P = .021). After subjects turned prone, mean VDS for posterior defects decreased significantly (P = .011), whereas those for anterior defects increased (P = .010). Most defects were less than 3 cm in diameter. It was found that immobilization of the subject for an extended period led to increased number of H(3)He ventilation defects in the dependent portions of the lung. Therefore, after a subject is positioned in the scanner, H(3)He MR imaging should be performed quickly to avoid the occurrence of the immobilization-induced ventilation defects and possible overestimation of disease.

  12. Hyperpolarized 3He MR imaging of the lung: Effect of subject immobilization on the occurrence of ventilation defects

    PubMed Central

    Mata, Jaime; Altes, Talissa; Knake, Jeffrey; Mugler, John; Brookeman, James; de Lange, Eduard

    2008-01-01

    Purpose To investigate immobilization-induced ventilation defects when performing hyperpolarized 3He (H3He) MRI of the lung. Methods and Materials Twelve healthy subjects underwent MRI of the lungs following inhalation of H3He gas at three time points: 1) immediately after having been positioned supine on the MR scanner table, 2) at 45 minutes while remaining supine, 3) and immediately thereafter after having turned prone. All image sets were reviewed in random order by three independent, blinded readers who recorded number, location and size of H3He ventilation defects. Scores were averaged for each time point and comparisons were made to determine change in number, location and size of ventilation defects with time and positioning of the subject in the scanner. Results At baseline supine there were small numbers of defects in the dependent (posterior) and non-dependent (anterior) portions of the lung (p=0.625). At 45 minutes there was a significant increase in the mean number of ventilation defects/slice (VDS) for the dependent (p=0.005) and a decrease for the non-dependent lung portions (p=0.021). After subjects turned prone, mean VDS for posterior defects decreased significantly (p=0.011) while those for anterior defects increased (p=0.010). Most defects were less than 3 cm in diameter. Conclusion It was found that immobilization of the subject for an extended period of time led to increased number of H3He ventilation defects in the dependent portions of the lung. Therefore, after a subject is positioned in the scanner, H3He MR imaging should be performed quickly to avoid the occurrence of the immobilization-induced ventilation defects, and possible overestimation of disease. PMID:18206626

  13. 3D 3He diffusion MRI as a local in vivo morphometric tool to evaluate emphysematous rat lungs

    PubMed Central

    Jacob, R. E.; Minard, K. R.; Laicher, G.; Timchalk, C.

    2008-01-01

    In this work, we investigate 3He magnetic resonance imaging as a noninvasive morphometric tool to assess emphysematous disease state on a local level. Emphysema was induced intratracheally in rats with 25 U/100 g body wt of porcine pancreatic elastase dissolved in 200 μl saline. Rats were then paired with saline-dosed controls. Nine three-dimensional (3D) 3He diffusion-weighted images were acquired at 1, 2, or 3 wk postdose, after which the lungs were harvested and prepared for histological analysis. Recently introduced indexes sensitive to the heterogeneity of the air space size distribution were calculated. These indexes, D1 and D2, were derived from the moments of the mean equivalent airway diameters. Averaged over the entire lung, it is shown that the average 3He diffusivity (Dave) correlates well with histology (R = 0.85, P < 0.0001). By matching small (0.046 cm2) regions in 3He images with corresponding regions in histological slices, Dave correlates significantly with both D1 and D2 (R = 0.88 and R = 0.90, respectively, with P < 0.0001). It is concluded that 3He MRI is a viable noninvasive morphometric tool for localized in vivo emphysema assessment. PMID:18719237

  14. Ventilation defects observed with hyperpolarized 3He magnetic resonance imaging in a mouse model of acute lung injury.

    PubMed

    Thomas, Abe C; Nouls, John C; Driehuys, Bastiaan; Voltz, James W; Fubara, Boma; Foley, Julie; Bradbury, J Alyce; Zeldin, Darryl C

    2011-05-01

    Regions of diminished ventilation are often evident during functional pulmonary imaging studies, including hyperpolarized gas magnetic resonance imaging (MRI), positron emission tomography, and computed tomography (CT). The objective of this study was to characterize the hypointense regions observed via (3)He MRI in a murine model of acute lung injury. LPS at doses ranging from 15-50 μg was intratracheally administered to C57BL/6 mice under anesthesia. Four hours after exposure to either LPS or saline vehicle, mice were imaged via hyperpolarized (3)He MRI. All images were evaluated to identify regions of hypointense signals. Lungs were then characterized by conventional histology, or used to obtain tissue samples from regions of normal and hypointense (3)He signals and analyzed for cytokine content. The characterization of (3)He MRI images identified three distinct types of hypointense patterns: persistent defects, atelectatic defects, and dorsal lucencies. Persistent defects were associated with the administration of LPS. The number of persistent defects depended on the dose of LPS, with a significant increase in mean number of defects in 30-50-μg LPS-dosed mice versus saline-treated control mice. Atelectatic defects predominated in LPS-dosed mice under conditions of low-volume ventilation, and could be reversed with deep inspiration. Dorsal lucencies were present in nearly all mice studied, regardless of the experimental conditions, including control animals that did not receive LPS. A comparison of (3)He MRI with histopathology did not identify tissue abnormalities in regions of low (3)He signal, with the exception of a single region of atelectasis in one mouse. Furthermore, no statistically significant differences were evident in concentrations of IL-1β, IL-6, macrophage inflammatory protein (MIP)-1α, MIP-2, chemokine (C-X-C motif) ligand 1 (KC), TNFα, and monocyte chemotactic protein (MCP)-1 between hypointense and normally ventilated lung regions in LPS

  15. 4-D segmentation and normalization of 3He MR images for intrasubject assessment of ventilated lung volumes

    NASA Astrophysics Data System (ADS)

    Contrella, Benjamin; Tustison, Nicholas J.; Altes, Talissa A.; Avants, Brian B.; Mugler, John P., III; de Lange, Eduard E.

    2012-03-01

    Although 3He MRI permits compelling visualization of the pulmonary air spaces, quantitation of absolute ventilation is difficult due to confounds such as field inhomogeneity and relative intensity differences between image acquisition; the latter complicating longitudinal investigations of ventilation variation with respiratory alterations. To address these potential difficulties, we present a 4-D segmentation and normalization approach for intra-subject quantitative analysis of lung hyperpolarized 3He MRI. After normalization, which combines bias correction and relative intensity scaling between longitudinal data, partitioning of the lung volume time series is performed by iterating between modeling of the combined intensity histogram as a Gaussian mixture model and modulating the spatial heterogeneity tissue class assignments through Markov random field modeling. Evaluation of the algorithm was retrospectively applied to a cohort of 10 asthmatics between 19-25 years old in which spirometry and 3He MR ventilation images were acquired both before and after respiratory exacerbation by a bronchoconstricting agent (methacholine). Acquisition was repeated under the same conditions from 7 to 467 days (mean +/- standard deviation: 185 +/- 37.2) later. Several techniques were evaluated for matching intensities between the pre and post-methacholine images with the 95th percentile value histogram matching demonstrating superior correlations with spirometry measures. Subsequent analysis evaluated segmentation parameters for assessing ventilation change in this cohort. Current findings also support previous research that areas of poor ventilation in response to bronchoconstriction are relatively consistent over time.

  16. Assessment of Regional Lung Function with Multivolume 1H MR Imaging in Health and Obstructive Lung Disease: Comparison with 3He MR Imaging

    PubMed Central

    Quirk, James D.; Yablonskiy, Dmitriy A.; Castro, Mario; Aliverti, Andrea; Woods, Jason C.

    2014-01-01

    Purpose To introduce a method based on multivolume proton (hydrogen [1H]) magnetic resonance (MR) imaging for the regional assessment of lung ventilatory function, investigating its use in healthy volunteers and patients with obstructive lung disease and comparing the outcome with the outcome of the research standard helium 3 (3He) MR imaging. Materials and Methods The institutional review board approved the HIPAA-compliant protocol, and informed written consent was obtained from each subject. Twenty-six subjects, including healthy volunteers (n = 6) and patients with severe asthma (n = 11) and mild (n = 6) and severe (n = 3) emphysema, were imaged with a 1.5-T whole-body MR unit at four lung volumes (residual volume [RVresidual volume], functional residual capacity [FRCfunctional residual capacity], 1 L above FRCfunctional residual capacity [FRC+1 L1 L above FRC], total lung capacity [TLCtotal lung capacity]) with breath holds of 10–11 seconds, by using volumetric interpolated breath-hold examination. Each pair of volumes were registered, resulting in maps of 1H signal change between the two lung volumes. 3He MR imaging was performed at FRC+1 L1 L above FRC by using a two-dimensional gradient-echo sequence. 1H signal change and 3He signal were measured and compared in corresponding regions of interest selected in ventral, intermediate, and dorsal areas. Results In all volunteers and patients combined, proton signal difference between TLCtotal lung capacity and RVresidual volume correlated positively with 3He signal (correlation coefficient R2 = 0.64, P < .001). Lower (P < .001) but positive correlation results from 1H signal difference between FRCfunctional residual capacity and FRC+1 L1 L above FRC (R2 = 0.44, P < .001). In healthy volunteers, 1H signal changes show a higher median and interquartile range compared with patients with obstructive disease and significant differences between nondependent and dependent regions. Conclusion Findings in this study

  17. Lung ventilation- and perfusion-weighted Fourier decomposition magnetic resonance imaging: in vivo validation with hyperpolarized 3He and dynamic contrast-enhanced MRI.

    PubMed

    Bauman, Grzegorz; Scholz, Alexander; Rivoire, Julien; Terekhov, Maxim; Friedrich, Janet; de Oliveira, Andre; Semmler, Wolfhard; Schreiber, Laura Maria; Puderbach, Michael

    2013-01-01

    The purpose of this work was to validate ventilation-weighted (VW) and perfusion-weighted (QW) Fourier decomposition (FD) magnetic resonance imaging (MRI) with hyperpolarized (3)He MRI and dynamic contrast-enhanced perfusion (DCE) MRI in a controlled animal experiment. Three healthy pigs were studied on 1.5-T MR scanner. For FD MRI, the VW and QW images were obtained by postprocessing of time-resolved lung image sets. DCE acquisitions were performed immediately after contrast agent injection. (3)He MRI data were acquired following the administration of hyperpolarized helium and nitrogen mixture. After baseline MR scans, pulmonary embolism was artificially produced. FD MRI and DCE MRI perfusion measurements were repeated. Subsequently, atelectasis and air trapping were induced, which followed with FD MRI and (3)He MRI ventilation measurements. Distributions of signal intensities in healthy and pathologic lung tissue were compared by statistical analysis. Images acquired using FD, (3)He, and DCE MRI in all animals before the interventional procedure showed homogeneous ventilation and perfusion. Functional defects were detected by all MRI techniques at identical anatomical locations. Signal intensity in VW and QW images was significantly lower in pathological than in healthy lung parenchyma. The study has shown usefulness of FD MRI as an alternative, noninvasive, and easily implementable technique for the assessment of acute changes in lung function. Copyright © 2012 Wiley Periodicals, Inc.

  18. Hyperpolarized 3He diffusion MRI and histology in pulmonary emphysema.

    PubMed

    Woods, Jason C; Choong, Cliff K; Yablonskiy, Dmitriy A; Bentley, John; Wong, Jonathan; Pierce, John A; Cooper, Joel D; Macklem, Peter T; Conradi, Mark S; Hogg, James C

    2006-12-01

    Diffusion MRI of hyperpolarized (3)He shows that the apparent diffusion coefficient (ADC) of (3)He gas is highly restricted in the normal lung and becomes nearly unrestricted in severe emphysema. The nature of this restricted diffusion provides information about lung structure; however, no direct comparison with histology in human lungs has been reported. The purpose of this study is to provide information about (3)He gas diffusivity in explanted human lungs, and describe the relationship between (3)He diffusivity and the surface area to lung volume ratio (SA/V) and mean linear intercept (L(m)) measurements--the gold standard for diagnosis of emphysema. Explanted lungs from patients who were undergoing lung transplantation for advanced COPD, and donor lungs that were not used for transplantation were imaged via (3)He diffusion MRI. Histological measurements were made on the same specimens after they were frozen in the position of study. There is an inverse correlation between diffusivity and SA/V (and a positive correlation between diffusivity and L(m)). An important result is that restricted (3)He diffusivity separated normal from emphysematous lung tissue more clearly than the morphometric analyses. This effect may be due to the smaller histologic sampling size compared to the MRI voxel sizes.

  19. In Vivo Lung Morphometry With Hyperpolarized 3He Diffusion MRI: Reproducibility and the Role of Diffusion Sensitizing Gradient Direction

    PubMed Central

    Quirk, James D.; Chang, Yulin V.; Yablonskiy, Dmitriy A.

    2014-01-01

    Purpose Lung morphometry with hyperpolarized gas diffusion MRI is a highly sensitive technique for the non-invasive measurement of acinar microstructural parameters traditionally only accessible by histology. The goal of this work is to establish the reproducibility of these measurements in healthy volunteers and their dependence upon the direction of the applied diffusion-sensitizing gradient. Methods Helium-3 lung morphometry MRI was performed on a total of five healthy subjects. Two subjects received duplicate imaging on the same day and three after a four or twenty-seven month delay to assess reproducibility. Four subjects repeated the measurement during the same session with different diffusion-sensitizing gradient directions to determine the effect on the parameter estimates. Results The helium-3 lung morphometry measurements were reproducible over the short and long term (e.g. % coefficient of variation (CV) of mean chord length, Lm = 2.1% and 2.9% respectively) and across different diffusion gradient directions (Lm % CV = 2.6%). Results also show independence of field inhomogeneity effects at 1.5T. Conclusion Helium-3 lung morphometry is a reproducible technique for measuring acinar microstructure and is effectively independent of the choice of diffusion gradient direction. This provides confidence for the use of this technique to compare populations and treatment efficacy. PMID:24752926

  20. A high-field 3He metastability exchange optical pumping polarizer operating in a 1.5 T medical scanner for lung magnetic resonance imaging

    NASA Astrophysics Data System (ADS)

    Collier, G.; Pałasz, T.; Wojna, A.; Głowacz, B.; Suchanek, M.; Olejniczak, Z.; Dohnalik, T.

    2013-05-01

    After being hyperpolarized using the technique of Metastability Exchange Optical Pumping (MEOP), 3He can be used as a contrast agent for lung magnetic resonance imaging (MRI). MEOP is usually performed at low magnetic field (˜1 mT) and low pressure (˜1 mbar), which results in a low magnetization production rate. Polarization preserving compression with a compression ratio of order 1000 is also required. It was demonstrated in sealed cells that high nuclear polarization values can be obtained at higher pressures with MEOP, if performed at high magnetic field (non-standard conditions). In this work, the feasibility of building a high-field polarizer that operates within a commercial 1.5 T scanner was evaluated. Preliminary measurements of nuclear polarization with sealed cells filled at different 3He gas pressures (1.33 to 267 mbar) were performed. The use of an annular shape for the laser beam increased by 25% the achievable nuclear polarization equilibrium value (Meq) at 32 and 67 mbar as compared to a Gaussian beam shape. Meq values of 66.4% and 31% were obtained at 32 and 267 mbar, respectively, and the magnetization production rate was increased by a factor of 10 compared to the best results obtained under standard conditions. To study the reproducibility of the method in a polarizing system, the same experiments were performed with small cells connected to a gas handling system. Despite careful cleaning procedure, the purity of the 3He gas could not be matched to that of the sealed cells. Consequently, the polarization build-up times were approximately 3 times longer in the 20-30 mbar range of pressure than those obtained for the 32 mbar sealed cell. However, reasonable Meq values of 40%-60% were achieved in a 90 ml open cell. Based on these findings, a novel compact polarizing system was designed and built. Its typical output is a 3He gas flow rate of 15 sccm with a polarization of 33%. In-vivo lung MRI ventilation images (Signal to Noise Ratio (SNR) of

  1. Hyperpolarized 129Xe MRI of the Human Lung

    PubMed Central

    Mugler, John P.; Altes, Talissa A.

    2012-01-01

    By permitting direct visualization of the airspaces of the lung, MR imaging using hyperpolarized gases provides unique strategies for evaluating pulmonary structure and function. Although the vast majority of research in humans has been performed using hyperpolarized 3He, recent contraction in the supply of 3He and consequent increases in price have turned attention to the alternative agent, hyperpolarized 129Xe. Compared to 3He, 129Xe yields reduced signal due to its smaller magnetic moment. Nonetheless, taking advantage of advances in gas-polarization technology, recent studies in humans using techniques for measuring ventilation, diffusion, and partial pressure of oxygen have demonstrated results for hyperpolarized 129Xe comparable to those previously demonstrated using hyperpolarized 3He. In addition, xenon has the advantage of readily dissolving in lung tissue and blood following inhalation, which makes hyperpolarized 129Xe particularly attractive for exploring certain characteristics of lung function, such as gas exchange and uptake, which cannot be accessed using 3He. Preliminary results from methods for imaging 129Xe dissolved in the human lung suggest that these approaches will provide new opportunities for quantifying relationships among gas delivery, exchange, and transport, and thus show substantial potential to broaden our understanding of lung disease. Finally, recent changes in the commercial landscape of the hyperpolarized-gas field now make it possible for this innovative technology to move beyond the research lab. PMID:23355432

  2. The 3He Supply Problem

    SciTech Connect

    Kouzes, Richard T.

    2009-05-01

    One of the main uses for 3He is in gas proportional counters for neutron detection. Radiation portal monitors deployed for homeland security and non-proliferation use such detectors. Other uses of 3He are for research detectors, commercial instruments, well logging detectors, dilution refrigerators, for targets or cooling in nuclear research, and for basic research in condensed matter physics. The US supply of 3He comes almost entirely from the decay of tritium used in nuclear weapons by the US and Russia. A few other countries contribute a small amount to the world’s 3He supply. Due to the large increase in use of 3He for homeland security, the supply has dwindled, and can no longer meet the demand. This white paper reviews the problems of supply, utilization, and alternatives.

  3. IEC-^3He Breeder for D-^3He Satellite Systems.

    NASA Astrophysics Data System (ADS)

    Chacon, L.; Miley, G. H.

    1996-11-01

    D-^3He fusion minimizes neutrons and maximizes charged fusion products, enabling increased energy recovery efficiency by direct conversion. However, scarce ^3He terrestrial resources have deterred R&D on this alternative. Here, we explore ^3He production through Inertial Electrostatic Confinement^1 (IEC) D-breeders, which supply ^3He to FRC D-^3He satellite reactors.^2 Favorable features for the IEC breeder include simplicity, low cost, easy extraction of fusion products, and compatibility with direct conversion. The breeder-satellite system energy balance is analyzed taking the net energy gain of the overall system, Q_N, as the figure of merit. Breeding is applicable for systems where the satellite Q-value, Q_S, > the breeder Q-value, Q_B. For improved performance, i.e., for high Q_N, QS >= QB >> 1 is needed; however, lower QB values (typical of the IEC) are permissible and still offer sufficient Q_N. An economic study determined breeding produces ^3He at a cost comparable to lunar ^3He, already shown to lead to competitive power.^3 The cost of electricity (COE) for the breeder-satellite complex was compared with the ARTEMIS COE,^4 using lunar ^3He fuel: assuming one satellite (1000 MWe)/breeder (170 MWe), the ratio of the breeding system COE to the lunar mining base COE is ~ 1.2. However, economic breeding is driven by large IEC breeder powers, i.e., increased ^3He breeding rates. Thus, the COE ratio approaches unity with two or three satellites/breeder, requiring increased breeder size and power (340 MWe for 2 satellites, 510 MWe for 3 satellites). Such systems potentially provide a ``bridge'' to a future lunar ^3He economy. 1. G.H. Miley et al., Dense Z-pinches, AIP Conf. 299, AIP Press, 675-689 (1994). 2. G.H. Miley, Nucl. Instrum. Methods, A271, 197-202 (1988). 3. L.J. Wittenberg et al., Fusion Technol., 10, 167-178 (1986). 4. H. Momota et al., Fusion Technol., 21, 2307-2323 (1992).

  4. 3He on preplated graphite

    NASA Astrophysics Data System (ADS)

    Gordillo, M. C.; Boronat, J.

    2016-10-01

    By using the diffusion Monte Carlo method, we obtained the full phase diagram of 3He on top of graphite preplated with a solid layer of 4He. All the 4He atoms of the substrate were explicitly considered and allowed to move during the simulation. We found that the ground state is a liquid of density 0.007 ±0.001 Å-2, in good agreement with available experimental data. This is significantly different from the case of 3He on clean graphite, in which both theory and experiment agree on the existence of a gas-liquid transition at low densities. Upon an increase in 3He density, we predict a first-order phase transition between a dense liquid and a registered 7/12 phase, the 4/7 phase being found metastable in our calculations. At larger second-layer densities, a final transition is produced to an incommensurate triangular phase.

  5. 4He/ 3He thermochronometry

    NASA Astrophysics Data System (ADS)

    Shuster, David L.; Farley, Kenneth A.

    2004-01-01

    Using classical diffusion theory, we present a mathematical technique for the determination of 4He concentration profiles in minerals. This approach should prove useful for constraining the low-temperature cooling histories of individual samples and for correcting (U-Th)/He ages for partial diffusive loss. The calculation assumes that the mineral of interest contains an artificially produced and uniform distribution of 3He obtained by proton irradiation [Shuster et al., Earth Planet. Sci. Lett. 217 (2004) 19-32]. In minerals devoid of natural helium, this isotope allows measurement of He diffusion coefficients; in minerals with measurable radiogenic He, it permits determination of 4He profiles arising during ingrowth and diffusion in nature. The 4He profile can be extracted from stepwise degassing experiments in which the 4He/ 3He ratio is measured. The evolution of the 4He/ 3He ratio as a function of cumulative 3He released can be compared with forward models to constrain the shape of the profile. Alternatively, we present a linear inversion that can be used to directly solve for the unknown 4He distribution. The inversion incorporates a standard regularization technique to filter the influence of random measurement errors on the solution. Using either approach we show that stepwise degassing data can yield robust and high-resolution information on the 4He profile. Profiles of radiogenic He are a sensitive function of the time-Temperate ( t- T) path that a cooling sample experienced. Thus, by step heating a proton-irradiated sample it is possible to restrict the sample's acceptable t- T paths. The sensitivity of this approach was explored by forward-modeling 4He profiles resulting from a range of realistic t- T paths, using apatite as an example. Results indicate that 4He profiles provide rich information on t- T paths, especially when the profiles are coupled with (U-Th)/He cooling ages on the same sample. Samples that experienced only moderate diffusive loss

  6. Threshold electrodisintegration of 3He

    NASA Astrophysics Data System (ADS)

    Hicks, R. S.; Hotta, A.; Churchwell, S.; Jiang, X.; Peterson, G. A.; Shaw, J.; Asavapibhop, B.; Berisso, M. C.; Bosted, P. E.; Burchesky, K.; Miskimen, R. A.; Rock, S. E.; Nakagawa, I.; Tamae, T.; Suda, T.; Golak, J.; Skibiński, R.; Witała, H.; Casagrande, F.; Turchinetz, W.; Cichocki, A.; Wang, K.; Glöckle, W.; Kamada, H.; Kobayashi, T.; Nogga, A.

    2003-06-01

    Cross sections were measured for the near-threshold electrodisintegration of 3He at momentum transfer values of q=2.4, 4.4, and 4.7 fm-1. From these and prior measurements the transverse and longitudinal response functions RT and RL were deduced. Comparisons are made against previously published and new nonrelativistic A=3 calculations using the best available nucleon-nucleon NN potentials. In general, for q<2 fm-1 these calculations accurately predict the threshold electrodisintegration of 3He. Agreement at increasing q demands consideration of two-body terms, but discrepancies still appear at the highest momentum transfers probed, perhaps due to the neglect of relativistic dynamics, or to the underestimation of high-momentum wave-function components.

  7. Polarisation and compression of {sup 3}He for Magnetic Resonance Imaging purposes

    SciTech Connect

    Geurts, D. G.; Brand, J. F. J. van den; Bulten, H. J.; Poolman, H. R.; Ferro-Luzzi, M.; Nicolay, K.

    1998-01-20

    Magnetic Resonance Imaging is often used in medical science as a diagnostic tool for the human body. Conventional MRI uses the NMR signal from the protons of water molecules in tissue to image the interior of the patient's body. However, for certain areas such as the lungs and airways, the usage of a highly polarised gas yields better results. We are currently constructing an apparatus that uses polarised {sup 3}He gas to produce detailed images of those signal-deficient moyeties. We also plan to study possible uptake of polarised {sup 3}He gas by the circulatory system to image other organs.

  8. T2-Shortening of 3He Gas by Magnetic Microspheres

    SciTech Connect

    Minard, Kevin R; Timchalk, Chuck; Corley, Rick A

    2005-03-01

    In the interconnected pores of a material like the lung the transverse relaxation time (T2) for 3He gas is shortened by the deposition of magnetic microspheres and rapid molecular diffusion through induced field distortions. Here, this unique relaxation process is described theoretically and predicted T2-shortening is validated using pressurized 3He gas in a foam model of lung tissue. Results demonstrate that – 1) significant T2-shortening is induced by microsphere deposition, 2) shortened T2’s are accurately predicted, and 3) measured relaxation times are exploitable for quantifying the local volume fraction of magnetic microspheres deposited in gas-filled spaces.

  9. Optical Pumping Spin Exchange {sup 3}He Gas Cells for Magnetic Resonance Imaging

    SciTech Connect

    Kim, W.; Stepanyan, S. S.; Kim, A.; Jung, Y.; Woo, S.; Yurov, M.; Jang, J.

    2009-08-04

    We present a device for spin-exchange optical pumping system to produce large quantities of polarized noble gases for Magnetic Resonance Imaging (MRI). A method and design of apparatus for pumping the polarization of noble gases is described. The method and apparatus enable production, storage and usage of hyperpolarized noble gases for different purposes, including Magnetic Resonance Imaging of human and animal subjects. Magnetic imaging agents breathed into lungs can be observed by the radio waves of the MRI scanner and report back physical and functional information about lung's health and desease. The technique known as spin exchange optical pumping is used. Nuclear magnetic resonance is implemented to measure the polarization of hyperpolarized gas. The cells prepared and sealed under high vacuum after handling Alkali metals into the cell and filling with the {sup 3}He-N{sub 2} mixture. The cells could be refilled. The {sup 3}He reaches around 50% polarization in 5-15 hours.

  10. Decoupling of Confined Normal 3He

    NASA Astrophysics Data System (ADS)

    Dimov, S. G.; Bennett, R. G.; Ilic, B.; Verbridge, S. S.; Levitin, L. V.; Fefferman, A. D.; Casey, A.; Saunders, J.; Parpia, J. M.

    2010-01-01

    Anodic bonding was used to fabricate a 10 mm diameter × 640 nm tall annular geometry suitable for torsion pendulum studies of confined 3He. For pure 3He at saturated vapor pressure the inertia of the confined fluid was seen to be only partially coupled to the pendulum at 160 mK. Below 100 mK the liquid’s inertial contribution was negligible, indicating a complete decoupling of the 3He from the pendulum.

  11. Improved Technique for Measurement of Regional Fractional Ventilation by Hyperpolarized 3He MRI

    PubMed Central

    Emami, Kiarash; Kadlecek, Stephen J.; Woodburn, John M.; Zhu, Jianliang; Yu, Jiangsheng; Vahdat, Vahid; Pickup, Stephen; Ishii, Masaru; Rizi, Rahim R.

    2012-01-01

    Quantitative measurement of regional lung ventilation is of great significance in assessment of lung function in many obstructive and restrictive pulmonary diseases. A new technique for regional measurement of fractional ventilation using hyperpolarized 3He MRI is proposed, addressing the shortcomings of an earlier approach that limited its use to small animals. The new approach allows for the acquisition of similar quantitative maps over a shortened period and requires substantially less 3He gas. This technique is therefore a better platform for implementation in large species, including humans. The measurements using the two approaches were comparable to a great degree, as verified in a healthy rat lung, and are very reproducible. Preliminary validation is performed in a lung phantom system. Volume dependency of measurements was assessed both in vivo and in vitro. A scheme for selecting an optimum flip angle is proposed. In addition, a dead space modeling approach is proposed to yield more accurate measurements of regional fractional ventilation using either method. Finally, sensitivity of the new technique to model parameters, noise, and number of included images were assessed numerically. As a prelude to application in humans, the technique was implemented in a large animal study successfully. PMID:19877277

  12. DNP for polarizing liquid {sup 3}He

    SciTech Connect

    Uemtasu, H.; Iwata, T.; Kato, S.; Michigami, T.; Ohizumi, S.; Shishido, T.; Tanaka, A.; Toyama, K.; Tajima, Y.; Yoshida, H. Y.; Kuriyama, N.

    2008-02-06

    Using DNP with zeolite powders and TEMPO, we have developed a method to enhance polarization of liquid {sup 3}He. At magnetic field of 2.5 T and a temperature of around 1.5 K, we have obatined polarization enhancement of liquid {sup 3}He, 2.34 and -1.59 for positive and negative enhancements, respectively.

  13. Vortices in rotating superfluid 3He

    PubMed Central

    Lounasmaa, Olli V.; Thuneberg, Erkki

    1999-01-01

    In this review we first present an introduction to 3He and to the ROTA collaboration under which most of the knowledge on vortices in superfluid 3He has been obtained. In the physics part, we start from the exceptional properties of helium at millikelvin temperatures. The dilemma of rotating superfluids is presented. In 4He and in 3He-B the problem is solved by nucleating an array of singular vortex lines. Their experimental detection in 3He by NMR is described next. The vortex cores in 3He-B have two different structures, both of which have spontaneously broken symmetry. A spin-mass vortex has been identified as well. This object is characterized by a flow of spins around the vortex line, in addition to the usual mass current. A great variety of vortices exist in the A phase of 3He; they are either singular or continuous, and their structure can be a line or a sheet or fill the whole liquid. Altogether seven different types of vortices have been detected in 3He by NMR. We also describe briefly other experimental methods that have been used by ROTA scientists in studying vortices in 3He and some important results thus obtained. Finally, we discuss the possible applications of experiments and theory of 3He to particle physics and cosmology. In particular, we report on experiments where superfluid 3He-B was heated locally by absorption of single neutrons. The resulting events can be used to test theoretical models of the Big Bang at the beginning of our universe. PMID:10393895

  14. Vortices in rotating superfluid 3He.

    PubMed

    Lounasmaa, O V; Thuneberg, E

    1999-07-06

    In this review we first present an introduction to 3He and to the ROTA collaboration under which most of the knowledge on vortices in superfluid 3He has been obtained. In the physics part, we start from the exceptional properties of helium at millikelvin temperatures. The dilemma of rotating superfluids is presented. In 4He and in 3He-B the problem is solved by nucleating an array of singular vortex lines. Their experimental detection in 3He by NMR is described next. The vortex cores in 3He-B have two different structures, both of which have spontaneously broken symmetry. A spin-mass vortex has been identified as well. This object is characterized by a flow of spins around the vortex line, in addition to the usual mass current. A great variety of vortices exist in the A phase of 3He; they are either singular or continuous, and their structure can be a line or a sheet or fill the whole liquid. Altogether seven different types of vortices have been detected in 3He by NMR. We also describe briefly other experimental methods that have been used by ROTA scientists in studying vortices in 3He and some important results thus obtained. Finally, we discuss the possible applications of experiments and theory of 3He to particle physics and cosmology. In particular, we report on experiments where superfluid 3He-B was heated locally by absorption of single neutrons. The resulting events can be used to test theoretical models of the Big Bang at the beginning of our universe.

  15. First result of the cross sectional measurement of 3He-3He solar reaction in OCEAN

    NASA Astrophysics Data System (ADS)

    Itahashi, T.; Kudomi, N.; Kume, K.; Takahisa, K.; Yoshida, S.; Ejiri, H.; Toki, H.; Nagai, Y.; Komori, M.; Ohsumi, H.

    2001-04-01

    The first result in OCEAN measurement of the fusion reactions 3He(3He,2p)α at the energy of 40 to 50 keV by means of a low-energy, high current accelerator are reported. The accelerator in this facility can produce an intense beam of 3He1+ and 3He2+ ions of more than 1mA. A detection efficiency for proposed detector assembly of ΔE-E counter telescope is simulated with GEANT program and it expects a detection efficiency about 10% for the two proton coincidence for 3He+3He-->2p+α reaction. The accuracy of Monte Carlo program was checked by D(3He,p)α reaction by replacing the target gas to deuterium. .

  16. Heterogeneous disease progression and treatment response in a C3HeB/FeJ mouse model of tuberculosis

    PubMed Central

    Lanoix, Jean-Philippe; Lenaerts, Anne J.; Nuermberger, Eric L.

    2015-01-01

    ABSTRACT Mice are the most commonly used species for non-clinical evaluations of drug efficacy against tuberculosis (TB). Unlike commonly used strains, C3HeB/FeJ mice develop caseous necrosis in the lung, which might alter the representation of drug efficacy in a way that is more like human TB. Because the development of such pathology requires time, we investigated the effect of infection incubation period on the activity of six drugs in C3HeB/FeJ and BALB/c mice. Mice were aerosol infected and held for 6, 10 or 14 weeks before receiving therapy with rifampin (RIF), rifapentine (RPT), pyrazinamide (PZA), linezolid (LZD), sutezolid (PNU) or metronidazole (MTZ) for 4-8 weeks. Outcomes included pathological assessments, pH measurements of liquefied caseum and assessment of colony-forming unit (CFU) counts from lung cultures. Remarkable heterogeneity in the timing and extent of disease progression was observed in C3HeB/FeJ mice, largely independent of incubation period. Likewise, drug efficacy in C3HeB/FeJ mice was not affected by incubation period. However, for PZA, LZD and PNU, dichotomous treatment effects correlating with the presence or absence of large caseous lesions were observed. In the case of PZA, its poor activity in the subset of C3HeB/FeJ mice with large caseous lesions might be explained by the pH of 7.36±0.09 measured in liquefied caseum. This study highlights the potential value of C3HeB/FeJ mice for non-clinical efficacy testing, especially for investigating the interaction of lesion pathology and drug effect. Careful use of this model could enhance the bridging of non-clinical results with clinical outcomes. PMID:26035868

  17. Heterogeneous disease progression and treatment response in a C3HeB/FeJ mouse model of tuberculosis.

    PubMed

    Lanoix, Jean-Philippe; Lenaerts, Anne J; Nuermberger, Eric L

    2015-06-01

    Mice are the most commonly used species for non-clinical evaluations of drug efficacy against tuberculosis (TB). Unlike commonly used strains, C3HeB/FeJ mice develop caseous necrosis in the lung, which might alter the representation of drug efficacy in a way that is more like human TB. Because the development of such pathology requires time, we investigated the effect of infection incubation period on the activity of six drugs in C3HeB/FeJ and BALB/c mice. Mice were aerosol infected and held for 6, 10 or 14 weeks before receiving therapy with rifampin (RIF), rifapentine (RPT), pyrazinamide (PZA), linezolid (LZD), sutezolid (PNU) or metronidazole (MTZ) for 4-8 weeks. Outcomes included pathological assessments, pH measurements of liquefied caseum and assessment of colony-forming unit (CFU) counts from lung cultures. Remarkable heterogeneity in the timing and extent of disease progression was observed in C3HeB/FeJ mice, largely independent of incubation period. Likewise, drug efficacy in C3HeB/FeJ mice was not affected by incubation period. However, for PZA, LZD and PNU, dichotomous treatment effects correlating with the presence or absence of large caseous lesions were observed. In the case of PZA, its poor activity in the subset of C3HeB/FeJ mice with large caseous lesions might be explained by the pH of 7.36±0.09 measured in liquefied caseum. This study highlights the potential value of C3HeB/FeJ mice for non-clinical efficacy testing, especially for investigating the interaction of lesion pathology and drug effect. Careful use of this model could enhance the bridging of non-clinical results with clinical outcomes.

  18. Cosmogenic 3He in detrital gold

    NASA Astrophysics Data System (ADS)

    Stuart, Finlay; Yakubovich, Olga; Caracedo, Ana; Nesterenok, Alexander

    2017-04-01

    Since the measurement of cosmogenic He in an alluvial diamond by McConville and Reynolds (1996) the application of cosmogenic noble gases to individual detrital grains to quantify surface processes has not been vigorously pursued. The likely low rate of diffusion of cosmogenic He in native metals, and their resistance to weathering and disintegration during erosion and transport, makes them a potential record of long-term Earth surface processes. In an effort to assess the extent that detrital refractory metals record the exposure history during transport and storage we have undertaken a reconnaissance study of the He isotope composition in 18 grains (2-200 mg) of native gold, copper, silver, and PtPd, Pt3Fe and OsIr alloys from alluvial placer deposits from around the world. 4He is dominantly the result of U and Th decay within the grains, or decay of 190Pt in the Pt-rich alloys. 3He is measurable in 13 grains, concentrations range up to 2.7E+6 atoms/g. 3He/4He are always in excess of the crustal radiogenic ratio, up to 306 Ra. Although nucleogenic 3He produced by (n,α) reactions on 6Li, and 3He from trapped hydrothermal fluids, are present, the majority of the 3He is cosmogenic in origin. Using newly calculated cosmogenic 3He production rates in heavy metals, and a determination of the effect of implantation based on the stopping distances of spallogenic 3He and 3H, the grains have 3Hecos concentrations that are equivalent to 0.35 to 1.5 Ma exposure at Earth's surface. In a study of detrital gold grains from several sites in Scotland we have found that 10 % have 3He concentrations that are significantly in excess of that generated since the Last Glacial Maximum. These studies demonstrate that, with refinement, cosmogenic 3He in refractory detrital minerals can be used to quantify sediment transport and storage on the 1-10 Ma timescale. P. McConville & J.H. Reynolds (1989). Geochim. Cosmochim. Acta, 53, 2365-75.

  19. Zircon 4He/3He thermochronometry

    NASA Astrophysics Data System (ADS)

    Tripathy-Lang, Alka; Fox, Matthew; Shuster, David L.

    2015-10-01

    Multiple thermochronometric methods are often required to constrain time-continuous rock exhumation for studying tectonic processes or development of km-scale topography at Earth's surface. Here, we explore 4He/3He thermochronometry of zircon as a method for constraining continuous time-temperature (t-T) paths of individual samples through a temperature range that is complementary to methods such as 40Ar/39Ar thermochronometry of K-feldspar and 4He/3He thermochronometry of apatite. For different cooling rates and diffusion domain size, the temperature sensitivity of zircon 4He/3He thermochronometry ranges from slightly less than 100 °C to slightly greater than 250 °C; a typical sample provides continuous thermal constraints over ∼100 °C within that range. Outside these temperatures, 4He in zircon will either be quantitatively retained or completely lost by volume diffusion. As proof-of-concept, we present stepwise release 4He/3He spectra and associated U and Th concentration maps measured by laser ablation ICP-MS analysis of individual crystal aliquots of Fish Canyon Tuff (FCT) zircon and of a more complex setting in the Sierra Nevada batholith that experienced reheating from a proximal basaltic intrusion, the Little Devil's Postpile (LDP). The FCT zircon 4He/3He release spectra are consistent with a 4He spatial distribution dominated by alpha-ejection from crystal surfaces. The spatial distributions of U and Th measured in the same crystals do not substantially influence 4He/3He release spectra that are predicted for the known thermal history, even when incorporating spatially variable diffusivity due to accumulation of radiation damage. Conversely, the LDP 4He/3He release spectra are strongly influenced by the observed parent nuclide zonation. A three-dimensional (3D) numerical model of 4He production and diffusion, which incorporates crystal geometry, U and Th zonation, and spatially variable He diffusion kinetics, substantially improves the fit between

  20. Translational applications of hyperpolarized 3He and 129Xe.

    PubMed

    Walkup, Laura L; Woods, Jason C

    2014-12-01

    Clinical magnetic resonance imaging of the lung is technologically challenging, yet over the past two decades hyperpolarized noble gas ((3)He and (129)Xe) imaging has demonstrated the ability to measure multiple pulmonary functional biomarkers. There is a growing need for non-ionizing, non-invasive imaging techniques due to increased concern about cancer risk from ionizing radiation, but the translation of hyperpolarized gas imaging to the pulmonary clinic has been stunted by limited access to the technology. New developments may open doors to greater access and more translation to clinical studies. Here we briefly review a few translational applications of hyperpolarized gas MRI in the contexts of ventilation, diffusion, and dissolved-phase imaging, as well as comparing and contrasting (3)He and (129)Xe gases for these applications. Simple static ventilation MRI reveals regions of the lung not participating in normal ventilation, and these defects have been observed in many pulmonary diseases. Biomarkers related to airspace size and connectivity can be quantified by apparent diffusion coefficient measurements of hyperpolarized gas, and have been shown to be more sensitive to small changes in lung morphology than standard clinical pulmonary functional tests and have been validated by quantitative histology. Parameters related to gas uptake and exchange and lung tissue density can be determined using (129)Xe dissolved-phase MRI. In most cases functional biomarkers can be determined via MRI of either gas, but for some applications one gas may be preferred, such as (3)He for long-range diffusion measurements and (129)Xe for dissolved-phase imaging. Greater access to hyperpolarized gas imaging coupled with newly developing therapeutics makes pulmonary medicine poised for a potential revolution, further adding to the prospects of personalized medicine already evidenced by advancements in molecular biology. Hyperpolarized gas researchers have the opportunity to

  1. Photoproduction of eta-mesic 3He.

    PubMed

    Pfeiffer, M; Ahrens, J; Annand, J R M; Beck, R; Caselotti, G; Cherepnya, S; Föhl, K; Fog, L S; Hornidge, D; Janssen, S; Kashevarov, V; Kondratiev, R; Kotulla, M; Krusche, B; McGeorge, J C; MacGregor, I J D; Mengel, K; Messchendorp, J G; Metag, V; Novotny, R; Rost, M; Sack, S; Sanderson, R; Schadmand, S; Thomas, A; Watts, D P

    2004-06-25

    The photoproduction of eta-mesic 3He has been investigated using the TAPS calorimeter at the Mainz Microtron accelerator facility MAMI. The total inclusive cross section for the reaction gamma3He-->etaX has been measured for photon energies from threshold to 820 MeV. The total and angular differential coherent eta cross sections have been extracted up to energies of 745 MeV. A resonancelike structure just above the eta production threshold with an isotropic angular distribution suggests the existence of a resonant quasibound state. This is supported by studies of a competing decay channel of such a quasibound eta-mesic nucleus into pi(0)pX. A binding energy of (-4.4+/-4.2) MeV and a width of (25.6+/-6.1) MeV is deduced for the quasibound eta-mesic state in 3He.

  2. Nuclear electric dipole moment of 3He

    SciTech Connect

    Stetcu, Ionel; Friar, J L; Hayes, A C; Liu, C P; Navratil, P

    2008-01-01

    In the no-core shell model (NCSM) framework, we calculate the {sup 3}He electric dipole moment (EDM) generated by parity- and time-reversal violation in the nucleon-nucleon interaction. While the results are somehow sensitive to the interaction model chosen for the strong two- and three-body interactions, we demonstrate the pion-exchange dominance to the EDM of {sup 3}He, if the coupling constants for {pi}, {rho} and {omega}-exchanges are of comparable magnitude, as expected. Finally, our results suggest that a measurement of {sup 3}He EDM would be complementary to the currently planned neutron and deuteron experiments, and would constitute a powerful constraint to the models of the pion P- and T-violating interactions.

  3. Coherent Photoproduction of pi^+ from 3/^He

    SciTech Connect

    Rakhsha Nasseripour, Barry Berman

    2011-03-01

    We have measured the differential cross section for the $\\gamma$$^3$He$\\rightarrow \\pi^+ t$ reaction. This reaction was studied using the CEBAF Large Acceptance Spectrometer (CLAS) at Jefferson Lab. Real photons produced with the Hall-B bremsstrahlung tagging system in the energy range from 0.50 to 1.55 GeV were incident on a cryogenic liquid $^3$He target. The differential cross sections for the $\\gamma$$^3$He$\\rightarrow \\pi^+ t$ reaction were measured as a function of photon-beam energy and pion-scattering angle. Theoretical predictions to date cannot explain the large cross sections except at backward angles, showing that additional components must be added to the model.

  4. Superfluid 3He in ``nematically ordered'' aerogel

    NASA Astrophysics Data System (ADS)

    Dmitriev, Vladimir

    2014-03-01

    Liquid 3He immersed in aerogel allows investigation of the influence of impurities on unconventional superfluidity. In most of such experiments silica aerogels are used. These aerogels consist of thin strands which form a ``wisp.'' Although it is established that superfluid phases of 3He in silica aerogels (A-like and B-like) have the same order parameters as A and B phases of bulk 3He, many new phenomena were observed. In particular, it was found that global anisotropy of aerogel (e.g. caused by squeezing or stretching) can orient the order parameter. Depending on prehistory and on the type of the anisotropy the A-like phase may be homogeneous or in a state with random orbital part of the order parameter. Theory predicts that a large stretching anisotropy may even influence the order parameter structure: polar phase (or A phase with polar distortion), which are not realized in bulk 3He, may become more favorable than pure A phase. Large stretching anisotropy is hardly achievable in silica aerogel. Therefore in experiments described in the talk we used a new type of aerogel, consisting of Al2O3 . H2O strands which are parallel to each other, i.e. this aerogel may be considered as infinitely stretched. We found that the superfluid phase diagram of 3He in such ``nematically ordered'' aerogel is different from the case of 3He in silica aerogel and that both observed A and B phases have large polar distortion. This distortion is larger at low pressures and grows on warming. There are indications that a pure polar phase appears near the superfluid transition temperature. Recent results will be also presented.

  5. The multiuniverse transition in superfluid 3He.

    PubMed

    Bunkov, Yury

    2013-10-09

    The symmetry-breaking phase transitions of the universe and of superfluid (3)He may lead to the formation of different states with different order parameters. In both cases the energy potential below the transition temperature has a complicated multidimensional profile with many local minima and saddle points, which correspond to different states. Consequently, not only topological defects, but also islands of different metastable states can be created. Using (3)He we can analyse the properties and experimental consequences of such transitions and, in particular, the first-order phase transition between the two low symmetry states.

  6. The multiuniverse transition in superfluid 3He

    NASA Astrophysics Data System (ADS)

    Bunkov, Yury

    2013-10-01

    The symmetry-breaking phase transitions of the universe and of superfluid 3He may lead to the formation of different states with different order parameters. In both cases the energy potential below the transition temperature has a complicated multidimensional profile with many local minima and saddle points, which correspond to different states. Consequently, not only topological defects, but also islands of different metastable states can be created. Using 3He we can analyse the properties and experimental consequences of such transitions and, in particular, the first-order phase transition between the two low symmetry states.

  7. Helium-3 Diffusion MR Imaging of the Human Lung over Multiple Time Scales

    PubMed Central

    Mugler, John P.; Wang, Chengbo; Miller, G. Wilson; Cates, Gordon D.; Mata, Jaime F.; Brookeman, James R.; de Lange, Eduard E.; Altes, Talissa A.

    2008-01-01

    Rationale and Objectives Diffusion MRI with hyperpolarized 3He gas is a powerful technique for probing the characteristics of the lung microstructure. A key parameter for this technique is the diffusion time, which is the period during which the atoms are allowed to diffuse within the lung for measurement of the signal attenuation. The relationship between diffusion time and the length scales that can be explored is discussed, and representative, preliminary results are presented from ongoing studies of the human lung for diffusion times ranging from milliseconds to several seconds. Materials and Methods 3He diffusion MR imaging of the human lung was performed on a 1.5T Siemens Sonata scanner. Using gradient-echo-based and stimulated-echo-based techniques for short and medium-to-long diffusion times, respectively, measurements were performed for times ranging from 2 ms to 6.5 s in two healthy subjects, a subject with sub-clinical chronic obstructive pulmonary disease and a subject with bronchopulmonary dysplasia. Results In healthy subjects, the apparent diffusion coefficient decreased by about 10-fold, from approximately 0.2 to 0.02 cm2/s, as the diffusion time increased from approximately 1 ms to 1 s. Results in subjects with disease suggest that measurements made at diffusion times substantially longer than 1 ms may provide improved sensitivity for detecting certain pathological changes in the lung microstructure. Conclusion With appropriately designed pulse sequences it is possible to explore the diffusion of hyperpolarized 3He in the human lung over more than a 1000-fold variation of the diffusion time. Such measurements provide a new opportunity for exploring and characterizing the microstructure of the healthy and diseased lung. PMID:18486006

  8. Evidence for Human Lung Stem Cells

    PubMed Central

    Kajstura, Jan; Rota, Marcello; Hall, Sean R.; Hosoda, Toru; D’Amario, Domenico; Sanada, Fumihiro; Zheng, Hanqiao; Ogórek, Barbara; Rondon-Clavo, Carlos; Ferreira-Martins, João; Matsuda, Alex; Arranto, Christian; Goichberg, Polina; Giordano, Giovanna; Haley, Kathleen J.; Bardelli, Silvana; Rayatzadeh, Hussein; Liu, Xiaoli; Quaini, Federico; Liao, Ronglih; Leri, Annarosa; Perrella, Mark A.; Loscalzo, Joseph; Anversa, Piero

    2011-01-01

    BACKGROUND Although progenitor cells have been described in distinct anatomical regions of the lung, description of resident stem cells has remained elusive. METHODS Surgical lung-tissue specimens were studied in situ to identify and characterize human lung stem cells. We defined their phenotype and functional properties in vitro and in vivo. RESULTS Human lungs contain undifferentiated human lung stem cells nested in niches in the distal airways. These cells are self-renewing, clonogenic, and multipotent in vitro. After injection into damaged mouse lung in vivo, human lung stem cells form human bronchioles, alveoli, and pulmonary vessels integrated structurally and functionally with the damaged organ. The formation of a chimeric lung was confirmed by detection of human transcripts for epithelial and vascular genes. In addition, the self-renewal and long-term proliferation of human lung stem cells was shown in serial-transplantation assays. CONCLUSIONS Human lungs contain identifiable stem cells. In animal models, these cells participate in tissue homeostasis and regeneration. They have the undemonstrated potential to promote tissue restoration in patients with lung disease. (Funded by the National Institutes of Health.) PMID:21561345

  9. Gaseous [sup 3]He-[sup 3]He magnetic dipolar spin relaxation

    SciTech Connect

    Newbury, N.R.; Barton, A.S.; Cates, G.D.; Happer, W.; Middleton, H. )

    1993-12-01

    We derive the nuclear-spin relaxation rate of gaseous [sup 3]He due to the magnetic-dipole interaction between the [sup 3]He nuclear spins. This dipolar relaxation rate is numerically evaluated for temperatures from 0.1 K to 550 K. At room temperature, the relaxation time for a [sup 3]He density of 10 amagats is 74.4 h. We have made a series of high-density (4--12 amagat) [sup 3]He samples for which nulcear relaxation is limited by the magnetic-dipole interaction. Both our theoretical and experimental results are particularly important for the growing use of [sup 3]He, polarized through spin exchange with optically pumped Rb vapor.

  10. Neutron Polarizers Based on Polarized 3He

    SciTech Connect

    William M. Snow

    2005-05-01

    The goal of this work, which is a collaborative effort between Indiana University, NIST, and Hamilton College, is to extend the technique of polarized neutron scattering into new domains by the development and application of polarized 3He-based neutron spin filters. After the IPNS experiment which measured Zeeman sp[litting in surface scattered neutrons using a polarized 3He cell as a polarization analyzer transporterd by car from Bloomington to Chicago, the Indiana work focused on technical developments to improve the 3He polarization of the Indiana compression system. The compression system was rebuilt with a new valve system which allows gas trapped in the dead volume of the compressors at the end of the piston stroke to be exhausted and conducted back to the optical pumping cell where it can be repolarized. We also incorporated a new intermediate storage volume made at NIST from 1720 glass which will reduce polarization losses between the compressors. Furthermore, we improved the stability of the 1083 nm laser by cooling the LMA rod. We achieved 60% 3he polarization in the optical pumping cell and 87% preservation of the polarization during compression. In parallel we built a magnetically-shielded transport solenoid for use on neutron scattering instruments such as POSY which achieves a fractional field uniformity of better than 10-3 per cm. The field was mapped using an automated 3D field mapping system for in-situ measurement of magnetic field gradients Diluted magnetic semiconductors offer many exciting opportunities for investigation of spintronic effects in solids and are certain to be one of the most active areas of condensed matter physics over then next several years. These materials can act as efficient spin injectors for devices that make use of spin-dependent transport phenomena. We just (late July 2002) finished a neutron reflectivity experiment at NIST on a GaMnAs trilayer film. This material is a ferromagnetic semiconductor which is of interest

  11. (3) He Spin Filter for Neutrons.

    PubMed

    Batz, M; Baeßler, S; Heil, W; Otten, E W; Rudersdorf, D; Schmiedeskamp, J; Sobolev, Y; Wolf, M

    2005-01-01

    The strongly spin-dependent absorption of neutrons in nuclear spin-polarized (3)He opens up the possibility of polarizing neutrons from reactors and spallation sources over the full kinematical range of cold, thermal and hot neutrons. This paper gives a report on the neutron spin filter (NSF) development program at Mainz. The polarization technique is based on direct optical pumping of metastable (3)He atoms combined with a polarization preserving mechanical compression of the gas up to a pressure of several bar, necessary to run a NSF. The concept of a remote type of operation using detachable NSF cells is presented which requires long nuclear spin relaxation times of order 100 hours. A short survey of their use under experimental conditions, e.g. large solid-angle polarization analysis, is given. In neutron particle physics NSFs are used in precision measurements to test fundamental symmetry concepts.

  12. 3He: cosmological and atomic physics experiments.

    PubMed

    Bunkov, Yuriy M

    2008-08-28

    Because the superfluid 3He order parameter exhibits many similarities with that of our Universe, the superfluid condensate may be considered as a quantum vacuum that carries various types of quasiparticles and topological defects. The condensate thus provides a test system for the experimental investigation of many general physics problems in cosmology, atomic or nuclear physics that are otherwise difficult or even impossible to investigate experimentally.

  13. Coupling between Solid 3He on Aerogel and Superfluid 3He in the Low Temperature Limit

    SciTech Connect

    Bradley, D. I.; Fisher, S. N.; Guenault, A. M.; Haley, R. P.; Pickett, G. R.; Tsepelin, V.; Whitehead, R. C. V.; Skyba, P.

    2006-09-07

    We have cooled liquid 3He contained in a 98% open aerogel sample surrounded by bulk superfluid 3He-B at zero pressure to below 120 {mu}K. The aerogel sample is placed in a quasiparticle blackbody radiator cooled by a Lancaster-style nuclear cooling stage to {approx}200 {mu}K. We monitor the temperature of the 3He inside the blackbody radiator using a vibrating wire resonator. We find that reducing the magnetic field on the aerogel sample causes substantial cooling of all the superfluid inside the blackbody radiator. We believe this is due to the demagnetization of the solid 3He layers on the aerogel strands. This system has potential for achieving extremely low temperatures in the confined fluid.

  14. Human lung lysozyme: sources and properties.

    PubMed

    Konstan, M W; Chen, P W; Sherman, J M; Thomassen, M J; Wood, R E; Boat, T F

    1981-01-01

    Lysozyme in human airway secretions is thought to defend the lung against airborne bacteria. Although lysozyme has been purified and characterized from human tears, milk, saliva, and other sources (1-5), human lung lysozyme has received little attention except for measurements of concentrations in sputum (6, 7), immunocytochemical and histochemical localization (8-12),and studies of secretion by alveolar macrophages (13). This study was designed to identify the sources of secreted lung lysozyme, to quantitate the secretory activities of the various sources,and to compare the properties of lysozyme from lung cells with those from other tissues.

  15. Intense polarized /sup 3/He ion source

    SciTech Connect

    Slobodrian, R.J.; Bertrand, R.; Grioux, J.; Labrie, R.; Lapainte, R.; Meunier, J.F.; Pigeon, G.; Pouliot, L.; Rioux, C.; Roy, R.

    1985-10-01

    This source is based on the atomic polarization of the 2/sup 3/S/sub 1/ metastable state of the neutral atom. A version suitable for operation on the high voltage terminal of a CN Van de Graaff has been constructed, bench tested and installed in the terminal of a 7.5 MV machine. The polarization of the atomic beam is higher than 90%. It is now fully operational and a current of /sup 3/He/sup +/ of 300 nA has been measured after acceleration.

  16. Neutron scattering from solid 3He

    NASA Astrophysics Data System (ADS)

    Schanen, R.; Sherline, T. E.; Toader, A. M.; Boyko, V.; Mat'as, S.; Meschke, M.; Schöttl, S.; Adams, E. D.; Cowan, B.; Godfrin, H.; Goff, J. P.; Roger, M.; Saunders, J.; Siemensmeyer, K.; Takano, Y.

    2003-05-01

    Multiple spin exchange leads, according to present understanding, to a variety of magnetically ordered states in solid 3He, depending on pressure and applied magnetic field. We report the status of experiments to directly determine these structures by neutron scattering. The large neutron absorption cross section, and associated sample heating, impose severe experimental demands on the design of the sample cell. We report on our proposed solution, including details of the sintered heat exchanger necessary to cool the sample, as well as the PrNi 5 nuclear demagnetization stage. The use of NMR in parallel experiments to characterise growth of the solid sample within the sinter is also discussed.

  17. 3He-melting-curve thermometry

    NASA Astrophysics Data System (ADS)

    Greywall, Dennis S.; Busch, Paul A.

    1982-03-01

    Precise measurements of the P-T relation along the melting curve of3He have been made for 8≲ T≲330 mK. The results are in excellent agreement with other precise data for temperatures near the extremes of this range. A best-fit relation is provided which describes the melting curve to within ±1 mbar between the superfluid A transition and the pressure minimum. Detailed descriptions of the melting curve and magnetic thermometers used for the calibration are also given.

  18. Development for the study of a Cross Sectional Measurement of 3He-3He Solar Reaction

    NASA Astrophysics Data System (ADS)

    Kudomi, N.; Itahashi, T.; Kume, K.; Takahisa, K.; Yoshida, S.; Ejiri, H.; Toki, H.; Nagai, Y.; Komori, M.; Ohsumi, H.

    2003-04-01

    The design and construction of a low-energy, high current accelerator for the study of fusion reactions are reported. The accelerator can produce an intense beam of 3He1+ and 3He2+ ions of more than 1mA. It enables us to provide extremely fine cross-section measurements of the 3He(3He,2p)α at 40 to 50 keV. A detection efficiency for proposed detector assembly of ΔE-E counter telescope is simulated with GEANT program and it expects a detection efficiency about 10% for the two proton coincidence for 3He+3He→2p+α. Deuter contaminations in target chamber is estimated to be less than ppm by quadrupole mass spectrometer. To further develop the study of nuclear astrophysics, a plasma target as an experimental apparatus for electron screening effects is proposed. Some parts of such apparatus are assembled. A combination ECR plasma target with a high current ion generator is under construction. The facility will be installed in the underground laboratory, Oto Cosmo Observatory. The facility has just started to operate and, as explained here, it already has been used for the double beta decay measurement and dark matter search programs. The present status of the experimental apparatus and its development are described.

  19. {sup 3}He melting pressure thermometry

    SciTech Connect

    Ni, W.; Xia, J.S.; Adams, E.D.

    1995-10-01

    High-precision measurements of the {sup 3}He melting pressure versus temperature have been made from 500 {mu}K to 25 mK using a {sup 60}Co nuclear orientation primary thermometer and a Pt NMR susceptibility secondary thermometer. Temperatures for the fixed points on the melting curve are: the superfluid A transition T{sub A}=2.505 mK, the A-B transition T{sub AB}=1.948 mK, and the solid ordering temperature T{sub N}=0.934 mK. These fixed points and a functional form for P(T) constitute a convenient temperature scale, based on a primary thermometer, usable to well below 1 mK.

  20. Cross section of 3He( 3He,2p) 4He measured near the Gamow peak

    NASA Astrophysics Data System (ADS)

    Itahashi, T.; Kudomi, N.; Yoshida, S.; Kume, K.; Komori, M.; Ohsumi, H.

    2003-06-01

    We measured the cross section of 3He( 3He,2p) 4He in the energy region of 30-50 keV center of mass energy by using the OCEAN facility. The resulting astrophysical S-factors are in agreement with the existing data and their total errors are as good as those of the previous experiment. For further study we tried the measurements at lower energies than E cm = 30 keV. It will require running times of one month with more improvements such as background reduction and steady operation of the detection system.

  1. Realization of administration unit for 3He with gas recycling

    NASA Astrophysics Data System (ADS)

    Güldner, M.; Becker, S.; Friesenecker, A.; Gast, K. K.; Heil, W.; Karpuk, S.; Otten, E. W.; Rivoire, J.; Salhi, Z.; Scholz, A.; Schreiber, L. M.; Terekhov, M.; Weiss, P.; Wolf, U.; Zentel, J.

    2011-06-01

    Hyperpolarized (HP) noble gases (3He,129Xe) are used for MR-imaging of the lung. In the majority of case the HP gas is filled in Tedlarbags and directly inhaled by the patients. Starting from an earlier pilot device, an administration unit was built respectively to the Medical Devices Law to administer patients HP noble gas boli in defined quantities and at a predefined time during inspiration with high reproducibility and reliability without reducing MR-quality. The patient's airflows are monitored and recorded. It is possible to use gas admixtures, measure the polarization on-line and collect the exhaled gas for later recycling. The first images with healthy volunteers were taken with this setup in a clinical study. Current results will be presented.

  2. Elastic Compton Scattering from 3He

    NASA Astrophysics Data System (ADS)

    Margaryan, Arman; Griesshammer, Harald W.; Phillips, Daniel R.; Strandberg, Bruno; McGovern, Judith A.; Shukla, Deepshikha

    2017-01-01

    We study elastic Compton scattering on 3He using chiral effective field theory (χEFT) at photon energies from 60 MeV to approximately 120 MeV. Experiments to measure this process have been proposed for both MAMI at Mainz and the HI γS facility at TUNL. I will present the revised results of a full calculation at third order in the expansion (O (Q3)). The amplitude involves a sum of both one- and two-nucleon Compton-scattering mechanisms. We have recently computed the fourth-order two-nucleon diagrams. The numerical impact they have on the cross-section results will be discussed. I will also present results in which amplitudes used so far are augmented by the leading effects from Δ (1232) degrees of freedom, a step which has already been performed for the proton and deuteron processes. Both cross sections and doubly-polarized asymmetries will be presented, and the sensitivity of these observables to the values of neutron scalar and spin polarizabilities will be assessed. This material is based upon work supported in part by DOE and George Washington University.

  3. Tiny Device Mimics Human Lung Function

    SciTech Connect

    McDonald, Rebecca; Harris, Jennifer; Nath, Pulak

    2016-04-25

    Scientists at Los Alamos National Laboratory are developing a miniature, tissue-engineered artificial lung that mimics the response of the human lung to drugs, toxins and other agents. “We breathe in and out thousands of times every day. And while we have control over what we eat or drink, we don’t always have control over what we breathe in,” said Jennifer Harris of Biosecurity and Public Health at Los Alamos, "and so we’re making this miniature lung to be able to test on actual human cells whether something in the environment, or a drug, is toxic or harmful to us." Nicknamed “PuLMo” for Pulmonary Lung Model (Pulmo is also the Latin word for "lung")the device consists of two major parts, the bronchiolar unit and the alveolar unit—just like the human lung. The units are primarily made from various polymers and are connected by a microfluidic “circuit board” that manages fluid and air flow. “When we build our lung, we not only take into account the aspects of different cell types, the tissues that are involved, we also take into account that a lung is supposed to breathe, so PuLMo actually breathes,” said Pulak Nath of Applied Modern Physics, who leads engineering efforts for the project. The most exciting application of PuLMo is a potentially revolutionary improvement in the reliability of drug-toxicity assessments and the prediction of new pharmaceutical success in humans, according to Harris. The PuLMo may also be designed to mimic lung disease conditions, such as Chronic Obstructive Pulmonary Disease (COPD) and asthma, and may be used to study lung air-flow dynamics to better understand the mechanisms of toxins and drug delivery and the effects of smoking, particularly the less-understood effects of e-cigarettes.

  4. Human lung ex vivo infection models.

    PubMed

    Hocke, Andreas C; Suttorp, Norbert; Hippenstiel, Stefan

    2017-03-01

    Pneumonia is counted among the leading causes of death worldwide. Viruses, bacteria and pathogen-related molecules interact with cells present in the human alveolus by numerous, yet poorly understood ways. Traditional cell culture models little reflect the cellular composition, matrix complexity and three-dimensional architecture of the human lung. Integrative animal models suffer from species differences, which are of particular importance for the investigation of zoonotic lung diseases. The use of cultured ex vivo infected human lung tissue may overcome some of these limitations and complement traditional models. The present review gives an overview of common bacterial lung infections, such as pneumococcal infection and of widely neglected pathogens modeled in ex vivo infected lung tissue. The role of ex vivo infected lung tissue for the investigation of emerging viral zoonosis including influenza A virus and Middle East respiratory syndrome coronavirus is discussed. Finally, further directions for the elaboration of such models are revealed. Overall, the introduced models represent meaningful and robust methods to investigate principles of pathogen-host interaction in original human lung tissue.

  5. Bedaquiline and Pyrazinamide Treatment Responses Are Affected by Pulmonary Lesion Heterogeneity in Mycobacterium tuberculosis Infected C3HeB/FeJ Mice

    PubMed Central

    2016-01-01

    BALB/c and Swiss mice are routinely used to validate the effectiveness of tuberculosis drug regimens, although these mouse strains fail to develop human-like pulmonary granulomas exhibiting caseous necrosis. Microenvironmental conditions within human granulomas may negatively impact drug efficacy, and this may not be reflected in non-necrotizing lesions found within conventional mouse models. The C3HeB/FeJ mouse model has been increasingly utilized as it develops hypoxic, caseous necrotic granulomas which may more closely mimic the pathophysiological conditions found within human pulmonary granulomas. Here, we examined the treatment response of BALB/c and C3HeB/FeJ mice to bedaquiline (BDQ) and pyrazinamide (PZA) administered singly and in combination. BALB/c mice consistently displayed a highly uniform treatment response to both drugs, while C3HeB/FeJ mice displayed a bimodal response composed of responsive and less-responsive mice. Plasma pharmacokinetic analysis of dissected lesions from BALB/c and C3HeB/FeJ mice revealed that PZA penetrated lesion types from both mouse strains with similar efficiency. However, the pH of the necrotic caseum of C3HeB/FeJ granulomas was determined to be 7.5, which is in the range where PZA is essentially ineffective under standard laboratory in vitro growth conditions. BDQ preferentially accumulated within the highly cellular regions in the lungs of both mouse strains, although it was present at reduced but still biologically relevant concentrations within the central caseum when dosed at 25 mg/kg. The differential treatment response which resulted from the heterogeneous pulmonary pathology in the C3HeB/FeJ mouse model revealed several factors which may impact treatment efficacy, and could be further evaluated in clinical trials. PMID:27227164

  6. Bedaquiline and Pyrazinamide Treatment Responses Are Affected by Pulmonary Lesion Heterogeneity in Mycobacterium tuberculosis Infected C3HeB/FeJ Mice.

    PubMed

    Irwin, Scott M; Prideaux, Brendan; Lyon, Edward R; Zimmerman, Matthew D; Brooks, Elizabeth J; Schrupp, Christopher A; Chen, Chao; Reichlen, Matthew J; Asay, Bryce C; Voskuil, Martin I; Nuermberger, Eric L; Andries, Koen; Lyons, Michael A; Dartois, Véronique; Lenaerts, Anne J

    2016-04-08

    BALB/c and Swiss mice are routinely used to validate the effectiveness of tuberculosis drug regimens, although these mouse strains fail to develop human-like pulmonary granulomas exhibiting caseous necrosis. Microenvironmental conditions within human granulomas may negatively impact drug efficacy, and this may not be reflected in non-necrotizing lesions found within conventional mouse models. The C3HeB/FeJ mouse model has been increasingly utilized as it develops hypoxic, caseous necrotic granulomas which may more closely mimic the pathophysiological conditions found within human pulmonary granulomas. Here, we examined the treatment response of BALB/c and C3HeB/FeJ mice to bedaquiline (BDQ) and pyrazinamide (PZA) administered singly and in combination. BALB/c mice consistently displayed a highly uniform treatment response to both drugs, while C3HeB/FeJ mice displayed a bimodal response composed of responsive and less-responsive mice. Plasma pharmacokinetic analysis of dissected lesions from BALB/c and C3HeB/FeJ mice revealed that PZA penetrated lesion types from both mouse strains with similar efficiency. However, the pH of the necrotic caseum of C3HeB/FeJ granulomas was determined to be 7.5, which is in the range where PZA is essentially ineffective under standard laboratory in vitro growth conditions. BDQ preferentially accumulated within the highly cellular regions in the lungs of both mouse strains, although it was present at reduced but still biologically relevant concentrations within the central caseum when dosed at 25 mg/kg. The differential treatment response which resulted from the heterogeneous pulmonary pathology in the C3HeB/FeJ mouse model revealed several factors which may impact treatment efficacy, and could be further evaluated in clinical trials.

  7. sup 3 He- sup 3 He dating: A case for mixing of young and old groundwaters

    SciTech Connect

    Kamensky, I.L.; Tolstikhin, I.N. ); Tokarev, I.V. )

    1991-10-01

    {sup 3}He/{sup 4}He and {sup 20}Ne/{sup 4}He ratios were measured in shallow underground waters (opened by water-supplying wells) of the Large Vud-Javr intramountain artesian basin in the Khibiny alkaline massif, the Kola Peninsula. The ratios vary from 1.321 {times} 10{sup {minus}6} to 2.065 {times} 10{sup {minus}6} and from 1.412 to 2.941, respectively, and a well-defined correlation is observed between them. Both these ratios in aquifers are known to be time-dependent, the former increases with time due to accumulation of {sup 3}He, produced in waters by {sup 3}H {beta}-decay; the latter decreases due to migration of helium from water-bearing rocks into the waters. The correlation is interpreted as a result of the mixing of two different types of waters. The approximation line enables the authors to estimate the isotopic ratios for the endmembers participating in the mixing and the mean residence time ({tau}) of tritigenic helium-3 in the water: (1) {sup 3}He/{sup 4}He = 3.655 {times} 10{sup {minus}6}, {sup 20}Ne/{sup 4}He = 4.03, and taking into consideration {sup 3}H concentrations in the well waters, {sup 3}H = 31.1 TU (practically the same for all samples), {tau} = 15.8 {plus minus} 1.5 years for the young water; (2) {sup 3}He/{sup 4}He = 0.20 {times} 10{sup {minus}6}, {sup 20}Ne/{sup 4}He = 0.18 and T = 0.11 Ma for the old one, the contribution of the old water being less than 10%. In one well a considerable contribution of modern-day meteoric water, about 16%, is observed.

  8. [Human lung connective tissue in postnatal ontogeny].

    PubMed

    Kasimtsev, A A; Nikolaev, V G

    1993-01-01

    Changes of the connective tissue structures, appearing during all postnatal ontogenesis stages were studied in 147 human lung specimens of different age groups (from newborns up to 82-year-olds). Qualitative and quantitative composition of connective tissue structures changes with the age which leads to the lateral aggregation of the fibers and growth of the general mass of the connective tissue. Heterochronia of the age variability manifestations in different regions of the lung framework was demonstrated. The original age transformations of connective tissue structures are characteristic for the basal lung regions. With the exception of perivasal connective tissue, similar changes in the region of the lung apexes appear 3-5 years later. This gives an opportunity to distinguish three anatomic zones in the lungs in an apico-basal direction, characterising the local nature of the age changes manifestations.

  9. Human papillomavirus and lung cancinogenesis: an overview.

    PubMed

    de Freitas, Antonio Carlos; Gurgel, Ana Pavla; de Lima, Elyda Golçalves; de França São Marcos, Bianca; do Amaral, Carolina Maria Medeiros

    2016-12-01

    Lung cancer is the most common cause of cancer deaths worldwide. Although tobacco smoking is considered to be the main risk factor and the most well-established risk factor for lung cancer, a number of patients who do not smoke have developed this disease. This number varies between 15 % to over one-half of lung cancer cases, and the deaths from lung cancer in non-smokers are increasing every year. There are many other agents that are thought to be etiological, including diesel exhaust exposure, metals, radiation, radon, hormonal factors, cooking oil, air pollution and infectious diseases, such as human papillomavirus (HPV). Studies in various parts of the world have detected HPV DNA at different rates in lung tumors. However, the role of HPV in lung cancer is still unclear. Thus, in this review, we investigated some molecular mechanisms of HPV protein activity in host cells, the entry of HPV into lung tissue and the possible route used by the virus to reach the lung cells.

  10. Pomeranchuk cell for hyperpolarized 3He based on the brute force method

    NASA Astrophysics Data System (ADS)

    Makino, Seiji; Tanaka, Masayoshi; Ueda, Kunihiro; Fujiwara, Mamoru; Fujimura, Hisako; Yosoi, Masaru; Ohta, Takeshi; Frossati, Giorgio; de Waard, Arlette; Rouille, Gerard

    2014-09-01

    MRI (Magnetic Resonance Imaging) has been used for the medical diagnosis as a radiation-free imaging equipment. Since the proton has been mainly used for medical MRI, usefulness has been rather restrictive. As an example for expanding the range of applicability, MRI with hyperpolarized 3He gas has been used for the lung disease. Here, ``hyperpolarized'' means ``polarized higher than the thermal equilibrium polarization.'' For producing a large amount of hyperpolarized 3He gas at a time, we have been developing a hyperpolarization technique based on the brute force method which uses an ultralow temperature of a few mK and a strong magnetic field around 17 T in combination with the principle of the Pomeranchuk cooling. The Pomeranchuk cell made with non-metallic materials of small heat capacity is attached to the 3He/4He dilution refrigerator using a sintered silver allowing large heat conduction. After the sensors to monitor the temperature and pressure of 3He are calibrated and the Pomeranchuk cell is constructed, the system is tested. Then, the solidification of 3He and the measurement of NMR (Nuclear Magnetic Resonance) signals of 3He under the magnetic field of 17 T are carried out. The current status is reported in this talk.

  11. Hyperpolarized (3)He pulmonary functional magnetic resonance imaging prior to radiation therapy.

    PubMed

    Mathew, Lindsay; Vandyk, Jake; Etemad-Rezai, Roya; Rodrigues, George; Parraga, Grace

    2012-07-01

    Radiation-induced lung injury (RILI) is the primary dose-limiting toxicity for radiation therapy of the lung, and although the effects of radiation dose on RILI development have been well characterized, the influence of chronic obstructive pulmonary disease (COPD) on the development of RILI and other outcomes is not well understood. The purpose of this small pilot study was to evaluate the relationship between hyperpolarized (3)He magnetic resonance imaging (MRI) measurements of COPD with RILI and 12-month survival in lung cancer patients undergoing radical radiotherapy and to evaluate the feasibility of pulmonary functional MRI as an image guidance∕planning tool for radiation therapy. Fifteen non-small cell and small cell lung cancer patients underwent pulmonary function tests, x-ray computed tomography (CT), and hyperpolarized (3)He MRI prior to radical radiation therapy (≥60 Gy). Conventional thoracic (1)H and hyperpolarized (3)He MRI were acquired to generate ventilation defect percent and the apparent diffusion coefficient for the ipsilateral and contralateral lungs independently. CT was acquired postradiation therapy and qualitatively evaluated for radiological evidence of RILI and 12-month survival was reported. Hyperpolarized (3)He MRI measurements of COPD classified 10∕15 subjects with contralateral lung COPD (CLC), and five subjects without COPD [contralateral lung normal (CLN)]. Of the 10 subjects with CLC, only four had a previous clinical diagnosis of COPD. CT images were acquired postradiation therapy for 13 subjects, and for eight (62%) of these there was qualitative evidence of RILI, including 5∕9 CLC and 3∕4 CLN subjects. The one-year survival was 2∕10 for CLC and 3∕5 for CLN subjects. In this small pilot study, we report the use of (3)He MRI to stratify lung cancer patients based on MRI evidence of COPD and showed that comorbid COPD was present in the majority of lung cancer subjects stratified for radiation therapy. Lung cancer

  12. Studies of 3He+3He, T+3He, and p +D nuclear reactions relevant to stellar or Big-Bang Nucleosynthesis using ICF plasmas at OMEGA

    NASA Astrophysics Data System (ADS)

    Zylstra, Alex; Gatu Johnson, Maria; Frenje, Johan; Li, Chikang; Seguin, Fredrick; Sio, Hong; Rosenberg, Michael; Rinderknecht, Hans; Petrasso, Richard; Herrmann, Hans; Kim, Yong Ho; Hale, Gerry; McNabb, Dennis; Sayre, Dan; Pino, Jesse; Brune, Carl; Bacher, Andy; Forrest, Chad; Glebov, Vladimir; Stoeckl, Christian; Janezic, Roger; Sangster, Craig

    2014-10-01

    The 3He+3He, T+3He, and p +D reactions directly relevant to Stellar or Big-Bang Nucleosynthesis (BBN) have been studied at the OMEGA laser facility using high-temperature low-density `exploding pusher' implosions. The advantage of using these plasmas is that they better mimic astrophysical systems than cold-target accelerator experiments. Measured proton spectra from the 3He3He reaction are used to constrain nuclear R-matrix modeling. The resulting T+3He γ-ray data rule out an anomalously-high 6Li production during BBN as an explanation to the high observed values in primordial material. The proton spectrum from the T+3He reaction is also being used to constrain the R-matrix model. Recent experiments have probed the p +D reaction for the first time in a plasma; this reaction is relevant to energy production in protostars, brown dwarfs and at higher CM energies to BBN. This work was partially supported by the US DOE, NLUF, LLE, and GA.

  13. Lung retention of cerium in humans.

    PubMed Central

    Pairon, J C; Roos, F; Iwatsubo, Y; Janson, X; Billon-Galland, M A; Bignon, J; Brochard, P

    1994-01-01

    A retrospective study was conducted to evaluate lung retention of particles containing cerium in subjects with and without previous occupational exposure to mineral dusts. Analytical transmission electron microscopy was performed on 459 samples of bronchoalveolar lavage (BAL) fluid and 75 samples of lung tissue. Study of the distribution of mineralogical species in human samples showed that particles containing cerium were encountered in less than 10% of subjects. The proportion of subjects with particles containing cerium in their biological samples was not different between controls and subjects with previous occupational exposure to fibrous or nonfibrous mineral dusts. This was considered as the background level of lung retention of cerium in the general population. By contrast, determination of the absolute concentration of particles containing cerium in BAL fluid and lung tissue samples showed that 1.2% (from BAL fluid) and 1.5% (from lung tissue) of subjects with previous exposure to mineral particles had high lung retention of particles containing cerium. This study is believed to be the first one in which lung retention of cerium was estimated in the general population. PMID:8130849

  14. Tiny Device Mimics Human Lung Function

    ScienceCinema

    McDonald, Rebecca; Harris, Jennifer; Nath, Pulak

    2016-07-12

    Scientists at Los Alamos National Laboratory are developing a miniature, tissue-engineered artificial lung that mimics the response of the human lung to drugs, toxins and other agents. “We breathe in and out thousands of times every day. And while we have control over what we eat or drink, we don’t always have control over what we breathe in,” said Jennifer Harris of Biosecurity and Public Health at Los Alamos, "and so we’re making this miniature lung to be able to test on actual human cells whether something in the environment, or a drug, is toxic or harmful to us." Nicknamed “PuLMo” for Pulmonary Lung Model (Pulmo is also the Latin word for "lung")the device consists of two major parts, the bronchiolar unit and the alveolar unit—just like the human lung. The units are primarily made from various polymers and are connected by a microfluidic “circuit board” that manages fluid and air flow. “When we build our lung, we not only take into account the aspects of different cell types, the tissues that are involved, we also take into account that a lung is supposed to breathe, so PuLMo actually breathes,” said Pulak Nath of Applied Modern Physics, who leads engineering efforts for the project. The most exciting application of PuLMo is a potentially revolutionary improvement in the reliability of drug-toxicity assessments and the prediction of new pharmaceutical success in humans, according to Harris. The PuLMo may also be designed to mimic lung disease conditions, such as Chronic Obstructive Pulmonary Disease (COPD) and asthma, and may be used to study lung air-flow dynamics to better understand the mechanisms of toxins and drug delivery and the effects of smoking, particularly the less-understood effects of e-cigarettes.

  15. Performance Limits of Pulse Tube Cryocoolers Using 3HE

    NASA Astrophysics Data System (ADS)

    Kittel, P.

    2008-03-01

    The enthalpy, entropy, and exergy flows resulting from the real gas effects of 3He in ideal pulse tube cryocoolers are described. The discussion follows a previous description of the real gas effects of 4He in ideal pulse tube cryocoolers and makes use of a recently developed model of the thermophysical properties of 3He. This model is used to describe how the thermodynamic flows are affected by real gas phenomena of 3He and compares these effects to similar effects for 4He. The analysis was done over the pressure range 0.3-2 MPa and temperatures down to 1 K. At 2 MPa there is almost no difference in the cooling power between 3He and 4He. At lower pressures, using 3He is advantageous. There is a 1-2 K reduction in the 3He cooling power vs. temperature curves compared to those for 4He in the 0.3-1 MPa range.

  16. /sup 3/He functions in tokamak-pumped laser systems

    SciTech Connect

    Jassby, D.L.

    1986-10-01

    /sup 3/He placed in an annular cell around a tokamak fusion generator can convert moderated fusion neutrons to energetic ions by the /sup 3/He(n,p)T reaction, and thereby excite gaseous lasants mixed with the /sup 3/He while simultaneously breeding tritium. The total /sup 3/He inventory is about 4 kg for large tokamak devices. Special configurations of toroidal-field magnets, neutron moderators and beryllium reflectors are required to permit nearly uniform neutron current into the laser cell with minimal attenuation. The annular laser radiation can be combined into a single output beam at the top of the tokamak.

  17. Rapid acquisition of helium-3 and proton three-dimensional image sets of the human lung in a single breath-hold using compressed sensing.

    PubMed

    Qing, Kun; Altes, Talissa A; Tustison, Nicholas J; Feng, Xue; Chen, Xiao; Mata, Jaime F; Miller, G Wilson; de Lange, Eduard E; Tobias, William A; Cates, Gordon D; Brookeman, James R; Mugler, John P

    2015-10-01

    To develop and validate a method for acquiring helium-3 ((3) He) and proton ((1) H) three-dimensional (3D) image sets of the human lung with isotropic spatial resolution within a 10-s breath-hold by using compressed sensing (CS) acceleration, and to assess the fidelity of undersampled images compared with fully sampled images. The undersampling scheme for CS acceleration was optimized and tested using (3) He ventilation data. Rapid 3D acquisition of both (3) He and (1) H data during one breath-hold was then implemented, based on a balanced steady-state free-precession pulse sequence, by random undersampling of k-space with reconstruction by means of minimizing the L1 norm and total variance. CS-reconstruction fidelity was evaluated quantitatively by comparing fully sampled and retrospectively undersampled image sets. Helium-3 and (1) H 3D image sets of the lung with isotropic 3.9-mm resolution were acquired during a single breath-hold in 12 s and 8 s using acceleration factors of 2 and 3, respectively. Comparison of fully sampled and retrospectively undersampled (3) He and (1) H images yielded mean absolute errors <10% and structural similarity indices >0.9. By randomly undersampling k-space and using CS reconstruction, high-quality (3) He and (1) H 3D image sets with isotropic 3.9-mm resolution can be acquired within an 8-s breath-hold. © 2014 Wiley Periodicals, Inc.

  18. Rapid Acquisition of Helium-3 and Proton 3D Image Sets of the Human Lung in a Single Breath-hold using Compressed Sensing

    PubMed Central

    Qing, Kun; Altes, Talissa A.; Tustison, Nicholas J.; Feng, Xue; Chen, Xiao; Mata, Jaime F.; Miller, G. Wilson; de Lange, Eduard E.; Tobias, William A.; Cates, Gordon D.; Brookeman, James R.; Mugler, John P.

    2015-01-01

    Purpose To develop and validate a method for acquiring helium-3 (3He) and proton (1H) three-dimensional (3D) image sets of the human lung with isotropic spatial resolution within a 10-s breath-hold by using compressed sensing (CS) acceleration, and to assess the fidelity of undersampled images compared to fully-sampled images. Methods The undersampling scheme for CS acceleration was optimized and tested using 3He ventilation data. Rapid 3D acquisition of both 3He and 1H data during one breath-hold was then implemented, based on a balanced steady-state free-precession pulse sequence, by random undersampling of k space with reconstruction via minimizing the L1 norm and total variance. CS-reconstruction fidelity was evaluated quantitatively by comparing fully-sampled and retrospectively-undersampled image sets. Results Helium-3 and 1H 3D image sets of the lung with isotropic 3.9-mm resolution were acquired during a single breath-hold in 12 s and 8 s using acceleration factors of 2 and 3, respectively. Comparison of fully-sampled and retrospectively-undersampled 3He and 1H images yielded mean absolute errors <10% and structural similarity indices >0.9. Conclusion By randomly undersampling k space and using CS reconstruction, high-quality 3He and 1H 3D image sets with isotropic 3.9-mm resolution can be acquired within an 8-s breath-hold. PMID:25335080

  19. MALDI Profiling of Human Lung Cancer Subtypes

    PubMed Central

    Nistal, Manuel; Calvo, Enrique; Madero, Rosario; Díaz, Esther; Camafeita, Emilio; de Castro, Javier; López, Juan Antonio; González-Barón, Manuel; Espinosa, Enrique; Fresno Vara, Juan Ángel

    2009-01-01

    Background Proteomics is expected to play a key role in cancer biomarker discovery. Although it has become feasible to rapidly analyze proteins from crude cell extracts using mass spectrometry, complex sample composition hampers this type of measurement. Therefore, for effective proteome analysis, it becomes critical to enrich samples for the analytes of interest. Despite that one-third of the proteins in eukaryotic cells are thought to be phosphorylated at some point in their life cycle, only a low percentage of intracellular proteins is phosphorylated at a given time. Methodology/Principal Findings In this work, we have applied chromatographic phosphopeptide enrichment techniques to reduce the complexity of human clinical samples. A novel method for high-throughput peptide profiling of human tumor samples, using Parallel IMAC and MALDI-TOF MS, is described. We have applied this methodology to analyze human normal and cancer lung samples in the search for new biomarkers. Using a highly reproducible spectral processing algorithm to produce peptide mass profiles with minimal variability across the samples, lineal discriminant-based and decision tree–based classification models were generated. These models can distinguish normal from tumor samples, as well as differentiate the various non–small cell lung cancer histological subtypes. Conclusions/Significance A novel, optimized sample preparation method and a careful data acquisition strategy is described for high-throughput peptide profiling of small amounts of human normal lung and lung cancer samples. We show that the appropriate combination of peptide expression values is able to discriminate normal lung from non-small cell lung cancer samples and among different histological subtypes. Our study does emphasize the great potential of proteomics in the molecular characterization of cancer. PMID:19890392

  20. Does diesel exhaust cause human lung cancer?

    PubMed

    Cox, L A

    1997-12-01

    Recent reviews of epidemiological evidence on the relation between exposure to diesel exhaust (DE) and lung cancer risk have reached conflicting conclusions, ranging from belief that there is sufficient evidence to conclude that DE is a human lung carcinogen (California EPA, 1994) to conclusions that there is inadequate evidence to support a causal association between DE and human lung cancer (Muscat and Wynder, 1995). Individual studies also conflict, with both increases and decreases in relative risks of lung cancer mortality being cited with 95% statistical confidence. On balance, reports of elevated risk outnumber reports of reduced risk. This paper reexamines the evidence linking DE exposures to lung cancer risk. After briefly reviewing animal data and biological mechanisms, it surveys the relevant epidemiological literature and examines possible explanations for the discrepancies. These explanations emphasize the distinction between statistical associations, which have been found in many studies, and causal associations, which appear not to have been established. Methodological threats to valid causal inference are identified and new approaches for controlling them are proposed using recent techniques from artificial intelligence (AI) and computational statistics. These threats have not been adequately controlled for in previous epidemiological studies. They provide plausible noncausal explanations for the reported increases in relative risks, making it impossible to infer causality between DE exposure and lung cancer risk from these studies. A key contribution is to show how recent techniques developed in the AI-and-statistics literature can help clarify the causal interpretation of complex multivariate data sets used in epidemiological risk assessments. Applied to the key study of Garshick et al. (1988), these methods show that DE concentration has no positive causal association with occupational lung cancer mortality risk.

  1. Early Days of Superfluid ^3He: An Experimenter's View

    NASA Astrophysics Data System (ADS)

    Lee, David

    2010-03-01

    The formulation of the BCS theory led theorists to investigate possible non-S-wave pairing in liquid ^3He. Unfortunately as time went on, estimates for the pairing temperature became unattainably low. Nevertheless, the push to lower temperatures by experimentalists continued and was facilitated by the invention of the dilution refrigerator. Nuclear adiabatic demagnetization could then be used to cool liquid ^3He to ˜1 mK as demonstrated by Goodkind. An alternate approach, suggested by Pomeranchuk, involved adiabatic compression of liquid ^3He into the solid phase. Efforts to develop this technique at the Kapitza Institute, La Jolla and Cornell achieved success in demonstrating cooling of mixtures of liquid and solid ^3He to ˜ 1 mK following dilution refrigerator pre-cooling. Although there was great pessimism regarding the possible observation of pairing in liquid ^3He, the unsettled problem of magnetic ordering in solid ^3He beckoned. Ultimately two phase transition along the melting curve were observed by Osheroff et al at Cornell. Although first associated with solid ^3He, extensive NMR studies showed them to be two new phases of liquid ^3He. A brief history of experiments at various laboratories following the discovery is given, along with early interpretations given by Anderson and Morel and Balian and Werthamer. The key role of Leggett's spin dynamics is also discussed.

  2. Dynamics of magnetic solitons in /sup 3/He-B

    SciTech Connect

    Rozhkov, S.S.

    1981-01-05

    The production and propagation of n-texture solitons in the B phase of /sup 3/He in a magnetic field is analyzed. It is conceivable that n solitons were observed in /sup 3/He-B by Webb, Sager, and Wheatley/sup 3/ in their experiments after the magnetic field was turned off.

  3. Comparison of lung preservation solutions in human lungs using an ex vivo lung perfusion experimental model.

    PubMed

    Medeiros, Israel L; Pêgo-Fernandes, Paulo M; Mariani, Alessandro W; Fernandes, Flávio G; Unterpertinger, Fernando V; Canzian, Mauro; Jatene, Fabio B

    2012-09-01

    Experimental studies on lung preservation have always been performed using animal models. We present ex vivo lung perfusion as a new model for the study of lung preservation. Using human lungs instead of animal models may bring the results of experimental studies closer to what could be expected in clinical practice. Brain-dead donors whose lungs had been declined by transplantation teams were used. The cases were randomized into two groups. In Group 1, Perfadex®was used for pulmonary preservation, and in Group 2, LPDnac, a solution manufactured in Brazil, was used. An ex vivo lung perfusion system was used, and the lungs were ventilated and perfused after 10 hours of cold ischemia. The extent of ischemic-reperfusion injury was measured using functional and histological parameters. After reperfusion, the mean oxygenation capacity was 405.3 mmHg in Group 1 and 406.0 mmHg in Group 2 (p = 0.98). The mean pulmonary vascular resistance values were 697.6 and 378.3 dyn·s·cm-5, respectively (p =0.035). The mean pulmonary compliance was 46.8 cm H20 in Group 1 and 49.3 ml/cm H20 in Group 2 (p =0.816). The mean wet/dry weight ratios were 2.06 and 2.02, respectively (p=0.87). The mean Lung Injury Scores for the biopsy performed after reperfusion were 4.37 and 4.37 in Groups 1 and 2, respectively (p = 1.0), and the apoptotic cell counts were 118.75/mm² and 137.50/mm², respectively (p=0.71). The locally produced preservation solution proved to be as good as Perfadex®. The clinical use of LPDnac may reduce costs in our centers. Therefore, it is important to develop new models to study lung preservation.

  4. Human Lung Immunity against Mycobacterium tuberculosis

    PubMed Central

    Schwander, Stephan; Dheda, Keertan

    2011-01-01

    The study of human pulmonary immunity against Mycobacterium tuberculosis (M.tb) provides a unique window into the biological interactions between the human host and M.tb within the broncho-alveolar microenvironment, the site of natural infection. Studies of bronchoalveolar cells (BACs) and lung tissue evaluate innate, adaptive, and regulatory immune mechanisms that collectively contribute to immunological protection or its failure. In aerogenically M.tb–exposed healthy persons lung immune responses reflect early host pathogen interactions that may contribute to sterilization, the development of latent M.tb infection, or progression to active disease. Studies in these persons may allow the identification of biomarkers of protective immunity before the initiation of inflammatory and disease-associated immunopathological changes. In healthy close contacts of patients with tuberculosis (TB) and during active pulmonary TB, immune responses are compartmentalized to the lungs and characterized by an exuberant helper T-cell type 1 response, which as suggested by recent evidence is counteracted by local suppressive immune mechanisms. Here we discuss how exploring human lung immunity may provide insights into disease progression and mechanisms of failure of immunological protection at the site of the initial host–pathogen interaction. These findings may also aid in the identification of new biomarkers of protective immunity that are urgently needed for the development of new and the improvement of current TB vaccines, adjuvant immunotherapies, and diagnostic technologies. To facilitate further work in this area, methodological and procedural approaches for bronchoalveolar lavage studies and their limitations are also discussed. PMID:21075901

  5. Hyperpolarized helium-3 mouse lung MRI: Studies of lung structure and function

    NASA Astrophysics Data System (ADS)

    Dugas, Joseph Paul

    Hyperpolarized 3He magnetic resonance imaging (MRI) of human and animal lungs has displayed promising and useful applications to studies of lung structure and function in both healthy and diseased lungs. Hyperpolarized 3He MRI allows the visualization of gas in the gas-exchange spaces of the lungs (as opposed to tissue) and has proven especially effective in studying diseases that are characterized by ventilation defects, such as emphysema. In particular, in-vivo measurements of the 3He apparent diffusion coefficient (ADC) can quantify lung structure by measuring its restrictive effects on the motion of 3He spins. This allows for detection and longitudinal tracking of changes in micro-architecture that result from disease destruction of alveolar walls. Due, in part, to the difficulties inherent in administering and imaging hyperpolarized 3He within the small (0.5 cc volume) mouse lung, applications of hyperpolarized 3He MRI techniques to laboratory mice are scarce. We have been able to implement and improve the techniques of hyperpolarized 3He mouse lung MRI and subsequently apply them to studies of several mouse models of disease, including elastase-induced emphysema, smoking-induced emphysema, and lung cancer. Here we detail the design, development, and implementation of a versatile, electronically-controlled, small animal ventilator that is capable of delivering tiny volumes of hyperpolarized 3He, mixed with oxygen, to the mouse and is also compatible with both the easily depolarized 3He gas and the highly magnetic environment within and around an imaging magnet. Also described are NM techniques developed to improve the signal-to-noise ratio of our images and effectively utilize the gas hyperpolarization. Applications of these technologies and techniques to small animal models of disease are presented wherein we have measured up to a 35% increase in 3He ADC in mice with elastase-induced emphysema as compared to healthy mice. We also demonstrate the potential

  6. The Map of 3He Abundance for the Moon Nearside

    NASA Astrophysics Data System (ADS)

    Kaydash, V. G.; Shkuratov, Yu. G.; Starukhina, L. V.

    1999-09-01

    In [1] strong correlation between 3He content in the lunar soils and the product of the soil maturity Is/FeO upon the content of TiO2 has been presented. Using this correlation we mapped the abundance of 3He for the nearside of the Moon. For this purpose the maps [2] of the maturity degree Is/FeO and abundance of titanium in the regolith of visible hemisphere were taken. The greatest 3He abundance is predicted for the west part of mare Serenitatis (16-20 ppb) associated with ilmenite basalts areas. The lowest 3He (1-5 ppb) is characteristic of the north and south highlands. As 3He is implanted by solar wind, the variation of its average normal flux with latitude and longitude of a lunar site could affect the 3He abundance. If so, 3He content near the poles should be less than that in equatorial zones. However, 3He content is controlled by degassing rates, which is proved by great difference between the abundance ratios of the gases in solar wind and in lunar soils [3]. Moreover, lower temperature in polar regions prevent degassing so effectively that even higher 3He content can be predicted there. As a first approximation we did not take the latitude and longitude dependence of 3He abundance into account. Refs.: [1] Taylor L.A. Engr., constr., & Oper. In Space IV, Proc. of Space'94, 678. [2] Shkuratov Yu.G. et al. 1999. Icarus, 137, 222-234. [3] Haskin L., Warren P. Lunar chemistry. Lunar sourcebook. /Eds. Heiken G. H. et al.. N. Y., 1991. 357-474.

  7. Solar Source Regions of Energetic 3He Emission

    NASA Astrophysics Data System (ADS)

    Mason, G. M.; Nitta, N. V.; Cohen, C. M.; Wiedenbeck, M. E.

    2012-12-01

    One of the surprising observations from the ACE mission has been the detection of energetic 3He emission occurring over multi-day periods. Previously observations of solar energetic 3He had detected short-lived "impulsive" energetic particle events which were associated with type III bursts and energetic electrons. The ACE observations were able to detect 3He at very low levels (<1% of 4He compared to ~10% in most earlier work) and this showed that the impulsive events often occurred during seemingly continuous multi-day periods of 3He emission. During solar active periods, 3He was present at 1 AU the majority of the time, giving evidence for either semi-continuous processes or else unresolved multiple small injections. The obvious injections during such periods were strongly associated with jet activity By adding STEREO and SDO observations we are seeking to extend the observational picture for these events. First, by following single 3He emitting regions from STEREO-B to ACE to STEREO-A we seek to examine for how long the 3He emission can continue, since any single spacecraft can be magnetically connected to a single region for only a few days and ACE often sees emission periods of that length. Second, by using SDO-AIA we seek to probe further the properties of the emitting regions to see if the previously reported association with jets is seen in events which we can now observe with greater resolution, sensitivity, and cadence than previously possible.

  8. ^3He neutron spin filters for polarized neutron scattering.

    NASA Astrophysics Data System (ADS)

    Chen, Wangchun; Borchers, Julie; Chen, Ying; O'Donovan, Kevin; Erwin, Ross; Lynn, Jeffrey; Majkrzak, Charles; McKenney, Sarah; Gentile, Thomas

    2006-03-01

    Polarized neutron scattering (PNS) is a powerful tool that probes the magnetic structures in a wide variety of magnetic materials. Polarized ^3He gas, produced by optical pumping, can be used to polarize or analyze neutron beams because of the strong spin dependence of the neutron absorption cross section for ^3He. Polarized ^3He neutron spin filters (NSF) have been of great interest in PNS community due to recent significant improvement of their performance. Here I will discuss successful applications using ^3He NSFs in polarized neutron reflectometry (PNR) and triple-axis spectrometry (TAS). In PNR, a ^3He NSF in conjunction with a position-sensitive detector allows for efficient polarization analysis of off-specular scattering over a broad range of reciprocal space. In TAS, a ^3He NSF in combination with a double focusing pyrolytic graphite monochromator provides greater versatility and higher intensity compared to a Heusler polarizer. Finally I will present the results from patterned magnetically-coupled thin films in PNR and our first ``proof-of-principle'' experiment in TAS, both of which were performed using ^3He NSF(s) at the NIST Center for Neutron Research.

  9. Precise measurement of the cross section of 3He ( 3He ,2p) 4He by using 3He doubly charged beam

    NASA Astrophysics Data System (ADS)

    Kudomi, Nobuyuki; Komori, Masataka; Takahisa, Keiji; Yoshida, Sei; Kume, Kyo; Ohsumi, Hideaki; Itahashi, Takahisa

    2004-01-01

    The fusion cross section of 3He ( 3He ,2p) 4He at a center of mass energy of 30 50 keV has been measured by using a helium-3 doubly ionized beam at a low-energy high current accelerator facility OCEAN. Free from molecular interference in the beam, the measurement determines the astrophysical S factor with better statistical and systematic errors than previous data. By using singly and doubly charged helium-3 ions, the facility envisages to provide the data from high-energy to Gamow energy regions.

  10. Neutron Detection Alternatives to 3He for National Security Applications

    SciTech Connect

    Kouzes, Richard T.; Ely, James H.; Erikson, Luke E.; Kernan, Warnick J.; Lintereur, Azaree T.; Siciliano, Edward R.; Stephens, Daniel L.; Stromswold, David C.; Van Ginhoven, Renee M.; Woodring, Mitchell L.

    2010-11-21

    One of the main uses for 3He is in gas proportional counters for neutron detection. Large radiation detection systems deployed for homeland security and proliferation detection applications use such systems. Due to the large increase in use of 3He for homeland security and basic research, the supply has dwindled, and can no longer meet the demand. This has led to the search for an alternative technology to replace the use of 3He-based neutron detectors. In this paper, we review the testing of currently commercially available alternative technologies for neutron detection in large systems used in various national security applications.

  11. Recent Advances of Polarized 3He Target at Jefferson Lab

    SciTech Connect

    Yi Qiang

    2011-10-01

    Polarized {sup 3}He target has been widely used in nuclear and particle experiments to study the neutron structure in the spin degree of freedom, as most of the {sup 3}He spin is carried by the unpaired neutron. Spin-Exchange Optical Pumping (SEOP) process is used in Jefferson Lab Hall A to polarize its {sup 3}He target. Through developments in recent years, both the performance and corresponding polarimetry of such a target were greatly improved. Several experiments recently carried out in Hall A benefited remarkably from this target for the record highest figure of merit.

  12. Experiments on polarization-dependent transport in 3He systems

    NASA Astrophysics Data System (ADS)

    Candela, D.; McAllaster, D. R.; Wei, L.-J.; Kalechofsy, N.

    1994-03-01

    Spin and momentum transport experiments are described for very dilute 3He- 4He mixtures and pure 3He brute-force polarized by a static field. Spin diffusion and rotation were observed in very dilute mixtures using a spin-wave resonance technique, and the viscosity increase due to polarization was observed using a vibrating wire. The mixture results are all well fit by the recent kinetic-equation calculations of Mullin and Jeon. Spin echoes were used to study transverse spin diffusion in pure 3He, providing the first clear evidence for polarization-induced relaxation-time anisotropy in a degenerate Fermi liquid.

  13. Chiral symmetry breaking in superfluid 3He-A.

    PubMed

    Ikegami, H; Tsutsumi, Y; Kono, K

    2013-07-05

    Spontaneous symmetry breaking is an important concept in many branches of physics. In helium-3 ((3)He), the breaking of symmetry leads to the orbital chirality in the superfluid phase known as (3)He-A. Chirality is a fundamental property of (3)He-A, but its direct detection has been challenging. We report direct detection of chirality by transport measurements of electrons trapped below a free surface of (3)He-A. In particular, we observed the so-called intrinsic Magnus force experienced by a moving electron; the direction of the force directly reflected the chirality. We further showed that, at the superfluid transition, the system selected either right- or left-handed chirality. The observation of such selection directly demonstrates chiral symmetry breaking.

  14. Apparatus for deformation tests of solids in liquid 3He

    NASA Astrophysics Data System (ADS)

    Hashimoto, T.; Katakura, S.; Edagawa, K.; Takeuchi, S.; Suzuki, T.

    2000-07-01

    An apparatus for deformation of solids in liquid 3He is constructed. Either tensile deformation or compression of a specimen can be performed by exchanging the assemblies in the 3He pot which has a capacity of about 30 cm3. The pulling rod for transmitting load from the tensile testing machine to the specimen runs inside the outlet tube of 3He, being isolated from 4He bath and almost free from mechanical friction. To measure the change in flow stress with the supernormal transition of superconducting metals, a superconducting magnet is mounted outside of the vacuum chamber which separates the 3He pot and the 4He bath. Under an applied load for plastic deformation the system is stably operative down to 0.6 K, while the lowest temperature achieved is 0.5 K. Some results on Ta and NaCl are presented.

  15. ^3He + ^3He measurement at E_cm = 45keV ~25keV

    NASA Astrophysics Data System (ADS)

    Itahashi, Takahisa; Komori, M.; Kudomi, N.; Yoshida, S.; Takahisa, K.

    2001-10-01

    A high brightness ion source and a precise low energy beam accelerator are indispensable tools in the study of fusion reactions in nuclear astrophysics. Of the reactions that follows the basic fusion in the sun, we have focused on the measurement of the ^3He+^3He reaction at the effective energy E_cm=17-27 keV. Currently the LUNA group has presented data down to 20.7 keV. The present paper describes the construction of a compact ion accelerator facility and results in the energy region of 25keV to 45keV. The experimental apparatus, OCEAN consists of (1) a powerful ion source that provides an intense current of ^3He^1+ or ^3He^2+ more than 1 mA at 30-50 keV (2) a low-energy beam transport with good transmission (30% for ^3He^1+ and 3% for ^3He^2+), (3) a windowless gas target and a circulation/purification system (4) a reliable calorimeter(accuracy 2%). (5) detectors, and (6) a data acquisition system. In the analysis, effective region for true reaction on E-ΔE plot was estimated as the followings. 1)The ^3He+^3He reaction was generated by simulation. 2)The Background contribution from ^3He+D reaction was generated by simulation, and those of cosmic rays, electrical noise and so on, were obtained by the background run. 3)ΔE and E distribution was divided into 16000 partitions, and signal to noise ratio were evaluated. From this procedure, S-factors were obtained as about 5 ~6 MeV \\cdotb.

  16. Development of a Polarized 3He Ion Source for RHIC

    SciTech Connect

    Milner, Richard G.

    2013-01-15

    The goal of the project was to design and construct a source of polarized 3He atoms for injection into EBIS. This is the initial step in producing polarized 3He beams in RHIC in collaboration with physicists from Columbia University and Brookhaven National Laboratory. These beams can be used to probe the spin structure of the neutron in the existing RHIC complex as well as to measure precisely the Bjorken Sum Rule at a future eRHIC electron-ion collider.

  17. Phase Shifts of p-3He Scattering at Low Energies

    NASA Astrophysics Data System (ADS)

    Yoshino, Y.; Limkaisang, V.; Nagata, J.; Yoshino, H.; Matsuda, M.

    2000-01-01

    A method employing single-energy phase-shift analysis of p-3He scattering is developed by using the S matrix in the Matsuda-Watari representation. This method can be applied for analyses in the low-energy region and also in the inelastic region. Phase-shift solutions of p-3He scattering are given at TL = 4.0, 5.5, 6.8, 8.5, 9.5 and 19.48 MeV.

  18. The SLAC E-154 {sup 3}He polarimeter

    SciTech Connect

    Romalis, M. V.; Bogorad, P. L.; Cates, G. D.; Kumar, K. S.; Chupp, T. E.; Coulter, K. P.; Smith, T. B.; Welsh, R.; Hughes, E. W.; Johnson, J. R.; Thompson, A. K.

    1998-01-20

    We describe the NMR and Rb Zeeman frequency shift polarimeters used for determining the {sup 3}He polarization in a recent precision measurement of the neutron spin structure function g{sub 1} at SLAC (E-154). We performed a detailed study of the systematic errors associated with the calibration of the NMR polarimeter. A new technique was used for determining the {sup 3}He polarization from the frequency shift of the Rb Zeeman resonance.

  19. Cytokine expression profile in human lungs undergoing normothermic ex-vivo lung perfusion.

    PubMed

    Sadaria, Miral R; Smith, Phillip D; Fullerton, David A; Justison, George A; Lee, Joon H; Puskas, Ferenc; Grover, Frederick L; Cleveland, Joseph C; Reece, T Brett; Weyant, Michael J

    2011-08-01

    A donor lung shortage prevents patients from receiving life-saving transplants. Ex-vivo lung perfusion (EVLP) is a viable means of expanding the donor pool by evaluating and potentially improving donor lung function. The metabolic and inflammatory effects of EVLP on human lung tissue are currently unknown. We sought to establish representative cytokine expression in human donor lungs meeting acceptable lung transplant criteria after prolonged normothermic EVLP. Seven single human lungs not meeting traditional transplantation criteria for various reasons underwent normothermic EVLP. Lungs were perfused with deoxygenated colloid, rewarmed, and ventilated per standard protocol. Lung function was evaluated every hour. Biopsies were taken at 1, 6, and 12 hours. Inflammatory cytokines were quantitatively measured using a human cytokine magnetic bead-based multiplex assay. All lungs met traditional transplant criteria after EVLP. The partial pressure of arterial oxygen and physiologic lung function significantly improved (p<0.05). No pulmonary edema was formed, and histology demonstrated no evidence of acute lung injury. Interleukin (IL)-6, IL-8, granulocyte colony-stimulating factor, and monocyte chemotactic protein-1 were upregulated, while granulocyte macrophage colony-stimulating factor was downregulated during EVLP (p<0.05). IL-1β, IL-4, IL-7, IL-12, interferon-γ, macrophage inflammatory protein-1β, and tumor necrosis factor-α were detectable and unchanged. Ex-vivo lung perfusion demonstrates the ability to improve oxygenation and physiologic lung function in donor lungs unacceptable for transplantation without injury to the lung. We establish here a cytokine expression profile in human lungs undergoing normothermic EVLP. These data can be used in the future to explore novel targeted therapies for ischemia-reperfusion injury. Copyright © 2011 The Society of Thoracic Surgeons. Published by Elsevier Inc. All rights reserved.

  20. Pickup coil optimization for polarized 3He system

    NASA Astrophysics Data System (ADS)

    Tu, X. Q.; Zheng, H.; Sun, G. A.; Gong, J.; Ren, Y.; Liu, L. J.; Gao, P. L.; Wang, W. Z.; Yan, H.

    2017-07-01

    Not only can polarized 3He gas work as neutron spin filters (NSF) but also search for spin-dependent new interactions beyond the standard model. For both cases, the relative polarization of the spin polarized noble gas needs to be measured precisely. Various NMR techniques are applied practically to monitor the 3He cell's polarization. In this work, we tried to optimize the pickup coil for the spin exchange optical pump (SEOP) based 3He system. By optimizing the signal-to-noise ratio (SNR), we found that the coil should be wound by thin wire for a higher number of turns. The optimized sizes of the pickup coils depend on the detailed configurations. For a spherical 3He cell with radius a, we found the optimized coil radius is √{5}(a+d), where d is the coil to cell surface distance. For a cylindrical 3He cell with height h, for the configurations where the coils were placed either along or perpendicular to the longitudinal axis of the target cell, we derived the optimized sizes for the specific configurations. We believe these results are practically useful for designing pickup coils of polarized 3He systems.

  1. Development of a 3He-hydraulic actuator for spin pump in superfluid 3He-A1

    NASA Astrophysics Data System (ADS)

    Yamaguchi, A.; Wada, M.; Tanaka, H.; Motoyama, G.; Sumiyama, A.; Aoki, Y.; Okuda, Y.; Murakawa, S.; Karaki, Y.; Kubota, M.; Kojima, H.

    2012-12-01

    The superfluid 3He A1 phase contains a spin-polarized condensate. This property allows novel superfluid spin current experiments. In the mechano-spin effect of the A1 phase a mechanically applied pressure gradient and a superleak-spin filter enable to directly boost spin polarization of 3He in a small chamber. Using a flexible membrane as an electrostatically actuated pump, we carried out such experiments and observed 50% enhancement of spin density. Here we report on a new 3He-hydraulic actuator for achieving greater enhancement of spin density. The actuator consists of two liquid 3He chambers located at a 4.2 K plate and in the interior of the cell. The pressure in the 4.2 K chamber is heater-controlled and it transmits a force onto a membrane in the cell. The motion of the membrane induces spin-polarized current into an accumulation chamber.

  2. Metabolic heterogeneity in human lung tumors

    PubMed Central

    Hensley, Christopher T.; Faubert, Brandon; Yuan, Qing; Lev-Cohain, Naama; Jin, Eunsook; Kim, Jiyeon; Jiang, Lei; Ko, Bookyung; Skelton, Rachael; Loudat, Laurin; Wodzak, Michelle; Klimko, Claire; McMillan, Elizabeth; Butt, Yasmeen; Ni, Min; Oliver, Dwight; Torrealba, Jose; Malloy, Craig R.; Kernstine, Kemp; Lenkinski, Robert E.; DeBerardinis, Ralph J.

    2015-01-01

    SUMMARY Non-small cell lung cancer (NSCLC) is heterogeneous in the genetic and environmental parameters that influence cell metabolism in culture. Here, we assessed the impact of these factors on human NSCLC metabolism in vivo using intra-operative 13C-glucose infusions in nine NSCLC patients to compare metabolism between tumors and benign lung. While enhanced glycolysis and glucose oxidation were common among these tumors, we observed evidence for oxidation of multiple nutrients in each of them, including lactate as a potential carbon source. Moreover, metabolically heterogeneous regions were identified within and between tumors, and surprisingly, our data suggested potential contributions of non-glucose nutrients in well-perfused tumor areas. Our findings not only demonstrate the heterogeneity in tumor metabolism in vivo but also highlight the strong influence of the microenvironment on this feature. PMID:26853473

  3. A novel SCID mouse model for studying spontaneous metastasis of human lung cancer to human tissue.

    PubMed

    Teraoka, S; Kyoizumi, S; Seyama, T; Yamakido, M; Akiyama, M

    1995-05-01

    We established a novel severe combined immunodeficient (SCID) mouse model for the study of human lung cancer metastasis to human lung. Implantation of both human fetal and adult lung tissue into mammary fat pads of SCID mice showed a 100% rate of engraftment, but only fetal lung implants revealed normal morphology of human lung tissue. Using these chimeric mice, we analyzed human lung cancer metastasis to both mouse and human lungs by subcutaneous inoculation of human squamous cell carcinoma and adenocarcinoma cell lines into the mice. In 60 to 70% of SCID mice injected with human-lung squamous-cell carcinoma, RERF-LC-AI, cancer cells were found to have metastasized to both mouse lungs and human fetal lung implants but not to human adult lung implants 80 days after cancer inoculation. Furthermore, human-lung adenocarcinoma cells, RERF-LC-KJ, metastasized to the human lung implants within 90 days in about 40% of SCID mice, whereas there were no metastases to the lungs of the mice. These results demonstrate the potential of this model for the in vivo study of human lung cancer metastasis.

  4. Morphometric examination of native lungs in human lung allograft recipients.

    PubMed

    Wiebe, B M; Burton, C M; Milman, N; Iversen, M; Andersen, C B

    2006-11-01

    The aim of the study was to estimate the degree of lung damage in patients with alpha(1)-antitrypsin (alpha1AT) deficiency, chronic obstructive pulmonary disease (COPD), and cystic fibrosis (CF) at the time of lung transplantation. Using unbiased stereological methods, lung-, bronchial- and vessel-volume, capillary length, and alveolar surface area and densities were estimated in recipient lungs from 21 consecutive patients with pre-transplant diagnoses including COPD (n=7), alpha1AT deficiency (n=6) and CF (n=8). Six unused adult donor lungs served as controls. Information relating to patient demography and pre-transplant lung function was obtained by retrospective chart review. Disease groups differed significantly with respect to demographics and pre-transplant lung function. Total lung volume was similar in all groups. Bronchial volume was significantly larger in CF patients compared to the control group (p<0.0001) and to the other two diagnostic groups: alpha1AT deficiency (p=0.0001) and COPD (p<0.0001). Alveolar surface density and capillary length density were significantly lower in patients with alpha1AT deficiency and COPD compared to controls (p<0.0001, respectively) and to patients with CF (p<0.0002, respectively). There were no correlations between clinical lung function and morphometric measurements. We conclude that unbiased microscopic stereological morphometry is an evolving science with the potential to elucidate pulmonary disease pathogenesis.

  5. Hyperpolarized 3He functional magnetic resonance imaging of bronchoscopic airway bypass in chronic obstructive pulmonary disease

    PubMed Central

    Mathew, Lindsay; Kirby, Miranda; Farquhar, Donald; Licskai, Christopher; Santyr, Giles; Etemad-Rezai, Roya; Parraga, Grace; McCormack, David G

    2012-01-01

    A 73-year-old exsmoker with Global initiative for chronic Obstructive Lung Disease stage III chronic obstructive pulmonary disease underwent airway bypass (AB) as part of the Exhale Airway Stents for Emphysema (EASE) trial, and was the only EASE subject to undergo hyperpolarized 3He magnetic resonance imaging for evaluation of lung function pre- and post-AB. 3He magnetic resonance imaging was acquired twice previously (32 and eight months pre-AB) and twice post-AB (six and 12 months post-AB). Six months post-AB, his increase in forced vital capacity was <12% predicted, and he was classified as an AB nonresponder. However, post-AB, he also demonstrated improvements in quality of life scores, 6 min walk distance and improvements in 3He gas distribution in the regions of stent placement. Given the complex relationship between well-established pulmonary function and quality of life measurements, the present case provides evidence of the value-added information functional imaging may provide in chronic obstructive pulmonary disease interventional studies. PMID:22332133

  6. Hyperpolarized 3He functional magnetic resonance imaging of bronchoscopic airway bypass in chronic obstructive pulmonary disease.

    PubMed

    Mathew, Lindsay; Kirby, Miranda; Farquhar, Donald; Licskai, Christopher; Santyr, Giles; Etemad-Rezai, Roya; Parraga, Grace; McCormack, David G

    2012-01-01

    A 73-year-old exsmoker with Global initiative for chronic Obstructive Lung Disease stage III chronic obstructive pulmonary disease underwent airway bypass (AB) as part of the Exhale Airway Stents for Emphysema (EASE) trial, and was the only EASE subject to undergo hyperpolarized 3He magnetic resonance imaging for evaluation of lung function pre- and post-AB. 3He magnetic resonance imaging was acquired twice previously (32 and eight months pre-AB) and twice post-AB (six and 12 months post-AB). Six months post-AB, his increase in forced vital capacity was <12% predicted, and he was classified as an AB nonresponder. However, post-AB, he also demonstrated improvements in quality of life scores, 6 min walk distance and improvements in 3He gas distribution in the regions of stent placement. Given the complex relationship between well-established pulmonary function and quality of life measurements, the present case provides evidence of the value-added information functional imaging may provide in chronic obstructive pulmonary disease interventional studies.

  7. Solar source regions of 3HE-rich particle events

    NASA Technical Reports Server (NTRS)

    Kahler, S. W.; Lin, R. P.; Reames, D. V.; Stone, R. G.; Liggett, M.

    1985-01-01

    Hydrogen alpha X-ray, and metric and kilometric radio data to examine the solar sources of energetic 3He-rich particle events observed near earth in association with impulsive 2 to 100 keV electron events were applied. Each 3He/electron event is associated with a kilometric type 3 burst belonging to a family of such bursts characterized by similar interplanetary propagation paths from the same solar active region. The 3He/electron events correlate very well with the interplanetary low frequency radio brightnesses of these events, but progressively worse with signatures from regions closer to the Sun. When H alpha brightnings can be associated with 3He/electron events, they have onsets coinciding to within 1 min of that of the associated metric type 3 burst but are often too small to be reported. The data are consistent with the earlier idea that many type 3 bursts, the 3He/electron events, are due to particle acceleration in the corona, well above the associated H alpha and X-ray flares.

  8. Search for muon catalyzed d 3He-fusion

    NASA Astrophysics Data System (ADS)

    Maev, E. M.; Balin, D. V.; Case, T.; Crowe, K. M.; Del Rosso, A.; Ganzha, V. A.; Hartmann, F. J.; Kozlov, S. M.; Lauss, B.; Maev, O. E.; Mühlbauer, M.; Mulhauser, F.; Petitjean, C.; Petrov, G. E.; Sadetsky, S. M.; Schapkin, G. N.; Schott, W.; Semenchuk, G. G.; Smirenin, Yu. V.; Soroka, M. A.; Vasiliev, A. A.; Vorobyov, A. A.; Voropaev, N. I.; Zmeskal, J.

    1999-06-01

    We report on the results of an experiment aimed at observing muon-catalyzed d 3He-fusion with a setup previously used for studies of the muon-catalyzed dd-fusion. The basic element of the setup is a high pressure ionization chamber operating as an active target. In this experiment the chamber was filled with an HD + 3He (5.6%) gas mixture at 13.2 bar pressure and 50 K temperature. These conditions were chosen as optimal for formation of the 3Heμd-molecules with a low level of background from the d-μ-d fusion. The chamber was exposed to the negative muon beam at PSI. During a 3-week data-taking period, 9.7 × 108 muon stops have been selected. The analysis of the data was able to determine a new upper limit for the d 3He-fusion rate in the 3Heμd-molecule (λf≤ 6× 104 s-1), which is more than three orders of magnitude lower than the previously existed limit.

  9. Experimental Search for μd 3He Fusion

    NASA Astrophysics Data System (ADS)

    Knowles, P. E.; Boreiko, V. M.; Bystritsky, V. M.; Filipowicz, M.; Huot, O.; Mulhauser, F.; Pavlov, V. N.; Penkov, F. M.; Petitjean, C.; Popov, N. P.; Sandukovsky, V. G.; Schaller, L. A.; Schneuwly, H.; Stolupin, V. A.; Woźniak, J.

    2001-12-01

    The vast majority of muon catalyzed fusion research has been concerned with muonic molecules of hydrogen isotopes only, since the dynamics of higher-Z muonic atoms in general preclude the formation of molecular systems. In the specific case of hydrogen helium mixtures, bound muonic molecular states can exist, and thus it is possible to search for the reaction μd 3He xrightarrow{{tilde λ f}} μ+α(3.66 MeV)+p(14.64 MeV). Until recently, the theoretical predictions for the nuclear fusion rate in the μd 3He molecule, {tilde λ } f , ranged over one order of magnitude, from 105 to 106 per second. An experimental upper limit has been measured for {tilde λ } f in HD + 3He giving a value (<6×104 s-1 [1]). We report on the analysis of an experiment in D2 + 3He which has shown a signal coming either from the muon catalyzed reaction, or from the fusion in flight of 3He's formed from dμd fusion.

  10. Optical Pumping / Spin Exchange ^3He Neutron Spin Filter Development

    NASA Astrophysics Data System (ADS)

    Hwang, Shenq-Rong; Coulter, Kevin P.; Chupp, Timothy E.; Welsh, Robert C.

    1998-04-01

    We have instrumented a thermal neutron beam line at the 2MW Ford reactor at the University of Michigan to develop a ^3He neutron spin filter test stand. Due to a large, spin depedent neutron cross section at low energies, polarized ^3He can be used as a neutron spin filter. Our ^3He spin filter is a 10 amagat-cm ^3He cell polarized via optical pumping/spin exchange with Rb. The filter is made of Corning 7056 glass filled with Rb , several atmosphere of ^3He and a few hundred torr nitrogen as buffer gas. We apply two 15W diode array lasers to optically pump Rb. In this presentation we will discuss some progress of this development, including a rotating oven design and a stepping motor driven neutron chopper. Preliminary results of the 10 amagat-cm filter will be presented and compared with theoretical calculations. A study of systematic errors from the data acquisition system and the neutron chopper will also be discussed.

  11. Polarized (3) He Spin Filters for Slow Neutron Physics.

    PubMed

    Gentile, T R; Chen, W C; Jones, G L; Babcock, E; Walker, T G

    2005-01-01

    Polarized (3)He spin filters are needed for a variety of experiments with slow neutrons. Their demonstrated utility for highly accurate determination of neutron polarization are critical to the next generation of betadecay correlation coefficient measurements. In addition, they are broadband devices that can polarize large area and high divergence neutron beams with little gamma-ray background, and allow for an additional spin-flip for systematic tests. These attributes are relevant to all neutron sources, but are particularly well-matched to time of flight analysis at spallation sources. There are several issues in the practical use of (3)He spin filters for slow neutron physics. Besides the essential goal of maximizing the (3)He polarization, we also seek to decrease the constraints on cell lifetimes and magnetic field homogeneity. In addition, cells with highly uniform gas thickness are required to produce the spatially uniform neutron polarization needed for beta-decay correlation coefficient experiments. We are currently employing spin-exchange (SE) and metastability-exchange (ME) optical pumping to polarize (3)He, but will focus on SE. We will discuss the recent demonstration of 75 % (3)He polarization, temperature-dependent relaxation mechanism of unknown origin, cell development, spectrally narrowed lasers, and hybrid spin-exchange optical pumping.

  12. Polarized 3He Spin Filters for Slow Neutron Physics

    PubMed Central

    Gentile, T. R.; Chen, W. C.; Jones, G. L.; Babcock, E.; Walker, T. G.

    2005-01-01

    Polarized 3He spin filters are needed for a variety of experiments with slow neutrons. Their demonstrated utility for highly accurate determination of neutron polarization are critical to the next generation of betadecay correlation coefficient measurements. In addition, they are broadband devices that can polarize large area and high divergence neutron beams with little gamma-ray background, and allow for an additional spin-flip for systematic tests. These attributes are relevant to all neutron sources, but are particularly well-matched to time of flight analysis at spallation sources. There are several issues in the practical use of 3He spin filters for slow neutron physics. Besides the essential goal of maximizing the 3He polarization, we also seek to decrease the constraints on cell lifetimes and magnetic field homogeneity. In addition, cells with highly uniform gas thickness are required to produce the spatially uniform neutron polarization needed for beta-decay correlation coefficient experiments. We are currently employing spin-exchange (SE) and metastability-exchange (ME) optical pumping to polarize 3He, but will focus on SE. We will discuss the recent demonstration of 75 % 3He polarization, temperature-dependent relaxation mechanism of unknown origin, cell development, spectrally narrowed lasers, and hybrid spin-exchange optical pumping. PMID:27308140

  13. 3He Neutron Detector Pressure Effect and Comparison to Models

    SciTech Connect

    Kouzes, Richard T.; Ely, James H.; Lintereur, Azaree T.; Siciliano, Edward R.; Stromswold, David C.; Woodring, Mitchell L.

    2010-01-14

    Reported here are the results of measurements performed to determine the efficiency of 3He filled proportional counters as a function of gas pressure in the SAIC system. Motivation for these measurements was largely to validate the current model of the SAIC system. Those predictions indicated that the neutron detection efficiency plotted as a function of pressure has a simple, logarithmic shape. As for absolute performance, the model results indicated the 3He pressure in the current SAIC system could not be reduced appreciably while meeting the current required level of detection sensitivity. Thus, saving 3He by reducing its pressure was predicted not to be a viable option in the current SAIC system.

  14. Ultrasensitive 3He magnetometer for measurements of high magnetic fields

    NASA Astrophysics Data System (ADS)

    Nikiel, Anna; Blümler, Peter; Heil, Werner; Hehn, Manfred; Karpuk, Sergej; Maul, Andreas; Otten, Ernst; Schreiber, Laura M.; Terekhov, Maxim

    2014-11-01

    We describe a 3He magnetometer capable to measure high magnetic fields ( B> 0.1 T) with a relative accuracy of better than 10-12. Our approach is based on the measurement of the free induction decay of gaseous, nuclear spin polarized 3He following a resonant radio frequency pulse excitation. The measurement sensitivity can be attributed to the long coherent spin precession time T2 ∗ being of order minutes which is achieved for spherical sample cells in the regime of "motional narrowing" where the disturbing influence of field inhomogeneities is strongly suppressed. The 3He gas is spin polarized in situ using a new, non-standard variant of the metastability exchange optical pumping. We show that miniaturization helps to increase T2 ∗ further and that the measurement sensitivity is not significantly affected by temporal field fluctuations of order 10-4.

  15. Neutron (3He) Spin Structure Functions at Low Q^2

    SciTech Connect

    Vincent Sulkosky

    2009-07-01

    Experiment E97-110 was performed at the Thomas Jefferson National Accelerator Facility to provide a precise measurement of the $^{3}$He spin structure functions at low $Q^{2}$ from 0.02 to 0.3~[GeV$/c$]$^{2}$. A longitudinally-polarized electron beam was scattered from a longitudinally or transversely polarized $^{3}$He target. From these data, we have extracted moments of the neutron and $^{3}$He spin structure functions at very low momentum transfers. These data allow us to make a benchmark check of Chiral Perturbation Theory calculations in a region where they are expected to be valid. In these proceedings, the experimental details are discussed and preliminary results on the first moments of the $g_1\\left(x,Q^{2}\\right)$ and $g_2\\left(x,Q^{2}\\right)$ structure functions are presented.

  16. Nonlinear acoustic effects in superfluid 3He-B

    NASA Astrophysics Data System (ADS)

    McKenzie, Ross H.; Sauls, J. A.

    1992-05-01

    We consider the nonlinear interaction of zero sound with the collective modes of the order-parameter in superfluid 3He-B. The approximate particle-hole symmetry of the 3He-Fermi liquid determines selection rules for the linear and nonlinear coupling of zero sound to the collective modes. Starting from the quasiclassical theory of superfluid 3He, we have shown that the coupling strenghts have a simple representation in terms of Feynman diagrams. We predict measurable two-photon absorption and nonlinear-Raman scattering by the J = 2 + (real squashing) modes at low pressures. Recent observations of two-phonon absorption by a group in Helsinki are compared to the theoretical predictions. Two-phonon absorption can be used to determine the dispersion of the J = 2 + modes.

  17. Nonlinear acoustics in superfluid 3He-B

    NASA Astrophysics Data System (ADS)

    McKenzie, Ross H.; Sauls, J. A.

    1989-10-01

    The nonlinear interaction of zero sound with the order parameter collective modes in superfluid 3He-B is considered within perturbation theory in the amplitude of the sound field. Selection rules for nonlinear excitation of the order parameter modes are determined by the approximate particle-hole symmetry of the 3He Fermi liquid. A diagrammatic algorithm, based on the quasiclassical theory of superfluid 3He, is used to calculate nonlinear coupling constants. These nonlinearities are sufficiently large that it should be possible to observe two phonon absorption and stimulated Raman scattering of zero sound by the real squashing (J=2+) mode. Finally, we discuss the possibility of using these nonlinearities to produce zero sound with `squeezed' noise.

  18. Quark-Hadron Duality in Neutron (3He) Spin Structure

    SciTech Connect

    Solvignon, Patricia; Liyanage, Nilanga; Chen, Jian-Ping; Choi, Seonho; Aniol, Konrad; Averett, Todd; Boeglin, Werner; Camsonne, Alexandre; Cates, Gordon; Chang, C.; Chang, C.C.; Chang, C.; Chang, C.C.; Chudakov, Eugene; Craver, Brandon; Cusanno, Francesco; Deur, Alexandre; Dutta, Dipangkar; Ent, Rolf; Feuerbach, Robert; Frullani, Salvatore; Gao, Haiyan; Garibaldi, Franco; Gilman, Ronald; Glashausser, Charles; Gorbenko, Viktor; Hansen, Jens-Ole; Higinbotham, Douglas; Ibrahim, Hassan; Jiang, Xiaodong; Jones, Mark; Kelleher, Aidan; Kelly, J.; Keppel, Cynthia; Kim, Wooyoung; Korsch, Wolfgang; Kramer, Kevin; Kumbartzki, Gerfried; LeRose, John; Lindgren, Richard; Ma, Bin; Margaziotis, Demetrius; Markowitz, Pete; McCormick, Kathy; Meziani, Zein-Eddine; Michaels, Robert; Moffit, Bryan; Monaghan, Peter; Munoz-Camacho, Carlos; Paschke, Kent; Reitz, Bodo; Saha, Arunava; Sheyor, Ran; Singh, Jaideep; Slifer, Karl; Sulkosky, Vince; Sulkosky, Vincent; Sulkosky, Vince; Sulkosky, Vincent; Tobias, William; Urciuoli, Guido; Wang, Kebin; Wijesooriya, Krishni; Wojtsekhowski, Bogdan; Woo, Seungtae; Yang, Jae-Choon; Zheng, Xiaochao; Zhu, Lingyan

    2008-10-01

    We present experimental results of the first high-precision test of quark-hadron duality in the spin-structure function g_1 of the neutron and $^3$He using a polarized 3He target in the four-momentum-transfer-squared range from 0.7 to 4.0 (GeV/c)^2. Global duality is observed for the spin-structure function g_1 down to at least Q^2 = 1.8 (GeV/c)^2 in both targets. We have also formed the photon-nucleon asymmetry A_1 in the resonance region for 3He and found no strong Q^2-dependence above 2.2 (GeV/c)^2.

  19. Feasibility of neutron diffraction on solid 3He

    NASA Astrophysics Data System (ADS)

    Siemensmeyer, K.; Schuberth, E. A.; Adams, E. D.; Takano, Y.; Guckelsberger, K.

    2000-07-01

    We have investigated the feasibility of neutron diffraction from solid 3He. The experiment will be performed at the HMI, first aiming for the properties of the antiferromagnetic ordering in the BCC phase and the ferromagnetic order in the HCP phase. Signal and beam heating considerations are essential to account for the enormous neutron absorption cross section of 3He. The study shows that neutron diffraction and transmission experiments are possible, relying on the experience gained from the neutron diffraction experiments on Cu and Ag at nanokelvin temperatures. A pressure cell has been developed which complies with the conflicting demands arising from the neutron and ultralow temperature aspects of the experiment. This work is a first step in an extensive effort to characterize 3He by neutron diffraction.

  20. Strong-Coupling and the Stripe Phase of ^3He

    NASA Astrophysics Data System (ADS)

    Wiman, Joshua J.; Sauls, J. A.

    2016-09-01

    Thin films of superfluid 3He were predicted, based on weak-coupling BCS theory, to have a stable phase which spontaneously breaks translational symmetry in the plane of the film. This crystalline superfluid, or "stripe" phase, develops as a one-dimensional periodic array of domain walls separating degenerate B phase domains. We report calculations of the phases and phase diagram for superfluid 3He in thin films using a strong-coupling Ginzburg-Landau theory that accurately reproduces the bulk 3He superfluid phase diagram. We find that the stability of the Stripe phase is diminished relative to the A phase, but the Stripe phase is stable in a large range of temperatures, pressures, confinement, and surface conditions.

  1. 3He melting-curve thermometry at millikelvin temperatures

    NASA Astrophysics Data System (ADS)

    Greywall, Dennis S.

    1985-03-01

    A pressure-versus-temperature calibration of the 3He melting curve is given for 13He [i.e., Tc(P)] as a basis for intercomparison, the melting-curve scale is found to be proportional to the magnetic temperature scales of Paulson et al. and Haavasoja et al. Included is a description of the PrNi5 nuclear demagnetization refrigerator which was used to cool the 3He samples to less than 0.3 mK.

  2. Quark-hadron duality in neutron (3He) spin structure.

    PubMed

    Solvignon, P; Liyanage, N; Chen, J-P; Choi, Seonho; Aniol, K; Averett, T; Boeglin, W; Camsonne, A; Cates, G D; Chang, C C; Chudakov, E; Craver, B; Cusanno, F; Deur, A; Dutta, D; Ent, R; Feuerbach, R; Frullani, S; Gao, H; Garibaldi, F; Gilman, R; Glashausser, C; Gorbenko, V; Hansen, O; Higinbotham, D W; Ibrahim, H; Jiang, X; Jones, M; Kelleher, A; Kelly, J; Keppel, C; Kim, W; Korsch, W; Kramer, K; Kumbartzki, G; Lerose, J J; Lindgren, R; Ma, B; Margaziotis, D J; Markowitz, P; McCormick, K; Meziani, Z-E; Michaels, R; Moffit, B; Monaghan, P; Munoz Camacho, C; Paschke, K; Reitz, B; Saha, A; Sheyor, R; Singh, J; Slifer, K; Sulkosky, V; Tobias, A; Urciuoli, G M; Wang, K; Wijesooriya, K; Wojtsekhowski, B; Woo, S; Yang, J-C; Zheng, X; Zhu, L

    2008-10-31

    We present experimental results of the first high-precision test of quark-hadron duality in the spin-structure function g_{1} of the neutron and 3He using a polarized 3He target in the four-momentum-transfer-squared range from 0.7 to 4.0 (GeV/c);{2}. Global duality is observed for the spin-structure function g_{1} down to at least Q;{2}=1.8 (GeV/c);{2} in both targets. We have also formed the photon-nucleon asymmetry A1 in the resonance region for 3He and found no strong Q2 dependence above 2.2 (GeV/c);{2}.

  3. 3He spin exchange cells for magnetic resonance imaging

    NASA Astrophysics Data System (ADS)

    Jacob, R. E.; Morgan, S. W.; Saam, B.

    2002-08-01

    We present a protocol for the consistent fabrication of glass cells to provide hyperpolarized (HP) 3He for pulmonary magnetic resonance imaging. The method for producing HP 3He is spin-exchange optical pumping. The valved cells must hold of order 1 atm[middle dot]L of gas at up to 15 atm pressure. Because characteristic spin-exchange times are several hours, the longitudinal nuclear relaxation time T1 for 3He must be several tens of hours and robust with respect to repeated refilling and repolarization. Collisions with the cell wall are a significant and often dominant cause of relaxation. Consistent control of wall relaxation through cell fabrication procedures has historically proven difficult. With the help of the discovery of an important mechanism for wall relaxation that involves magnetic surface sites in the glass, and with the further confirmation of the importance of Rb metal to long wall-relaxation times, we have developed a successful protocol for fabrication of 3He spin exchange cells from inexpensive and easily worked borosilicate (Pyrex) glass. The cells are prepared under vacuum using a high-vacuum oil-free turbomolecular pumping station, and they are sealed off under vacuum after [greater-than-or-equal, slanted]100 mg of distilled Rb metal is driven in. Filling of cells with the requisite 3He-N2 mixture is done on an entirely separate gas-handling system. Our cells can be refilled and the gas repolarized indefinitely with no significant change in their wall properties. Relaxation data are presented for about 30 cells; the majority of these reach a "40/40" benchmark: T1>40 h, and 3He polarizations reach or exceed 40%. Typical polarization times range from 12 to 20 h; 20% polarization can be achieved in 3-5 h.

  4. Neutron-scattering experiment on solid 3He

    NASA Astrophysics Data System (ADS)

    Mat'aš, S.; Bat'ko, I.; Boyko, V.; Schöttl, S.; Siemensmeyer, K.; Raasch, S.; Radulov, I.; Adams, E. D.; Scherline, T. E.

    The central aim of our work is the characterisation of magnetic and crystallographic properties of solid 3He on a microscopic scale. This can only be achieved using neutron-diffraction techniques. The potential of neutron methods in magnetism and their application to nuclear magnetism is well known. They were very successful in the recent investigation of spontaneous nuclear order in copper and silver. The high neutron absorption cross section makes the application of neutron diffraction in solid 3He very difficult - but a careful feasibility study of diffraction experiments shows that new results of fundamental importance in the field of magnetism may be gained.

  5. Anisotropic Phases of Superfluid 3He in Compressed Aerogel

    NASA Astrophysics Data System (ADS)

    Li, J. I. A.; Zimmerman, A. M.; Pollanen, J.; Collett, C. A.; Halperin, W. P.

    2015-03-01

    It has been shown that the relative stabilities of various superfluid states of 3He can be influenced by anisotropy in a silica aerogel framework. We prepared a suite of aerogel samples compressed up to 30% for which we performed pulsed NMR on 3He imbibed within the aerogel. We identified A and B phases and determined their magnetic field-temperature phase diagrams as a function of strain. From these results, we infer that the B phase is distorted by negative strain forming an anisotropic superfluid state more stable than the A phase.

  6. A Study of 3He detectors for Active Interrogation

    SciTech Connect

    E.H. Seabury; D.L. Chichester

    2009-10-01

    3He proportional counters have long been used as neutron detectors for both passive and active detection of Special Nuclear Material (SNM). The optimal configuration of these detectors as far as gas pressure, amount of moderating material, and size are concerned is highly dependent on what neutron signatures are being used to detect and identify SNM. We present here a parametric study of the neutron capture response of 3He detectors, based on Monte Carlo simulations using the MCNPX radiation transport code. The neutron capture response of the detectors has been modeled as a function of time after an incident neutron pulse.

  7. Removing gaseous contaminants in {sup 3}He by cryogenic stripping

    SciTech Connect

    Benapfl, M.; Biltoft, P.; Coombs, A.

    1995-08-17

    The Tritium Operations Group at LLNL, Tritium Facility has recently developed a {sup 3}He recovery system to remove argon, xenon, neon, hydrogen, and all other contaminants from the {sup 3}He stream in an Accelerator Production of Tritium (APT) experimental apparatus. In this paper the authors will describe in detail the background information, technical requirements, the design approach, and the results of their experimental tests. The authors believe this gas purification system may have other applications as it provides at a reasonable cost an efficient method for purification of gaseous helium.

  8. Lung flooding enables efficient lung sonography and tumour imaging in human ex vivo and porcine in vivo lung cancer model

    PubMed Central

    2013-01-01

    Background Sonography has become the imaging technique of choice for guiding intraoperative interventions in abdominal surgery. Due to artefacts from residual air content, however, videothoracoscopic and open intraoperative ultrasound-guided thermoablation of lung malignancies are impossible. Lung flooding is a new method that allows complete ultrasound imaging of lungs and their tumours. Methods Fourteen resected tumourous human lung lobes were examined transpleurally with B-mode ultrasound before (in atelectasis) and after lung flooding with isotonic saline solution. In two swine, the left lung was filled with 15 ml/kg isotonic saline solution through the left side of a double-lumen tube. Lung tumours were simulated by transthoracic ultrasound-guided injection of 5 ml of purified bovine serum albumin in glutaraldehyde, centrally into the left lower lung lobe. The rate of tumour detection, the severity of disability caused by residual gas, and sonomorphology of the lungs and tumours were assessed. Results The ex vivo tumour detection rate was 100% in flooded human lung lobes and 43% (6/14) in atelectatic lungs. In all cases of atelectasis, sonographic tumour imaging was impaired by residual gas. Tumours and atelectatic tissue were isoechoic. In 28% of flooded lungs, a little residual gas was observed that did not impair sonographic tumour imaging. In contrast to tumours, flooded lung tissue was hyperechoic, homogeneous, and of fine-grained structure. Because of the bronchial wall three-laminar structure, sonographic differentiation of vessels and bronchi was possible. In all cases, malignant tumours in the flooded lung appeared well-demarcated from the lung parenchyma. Adenocarcinoma, squamous, and large cell carcinomas were hypoechoic. Bronchioloalveolar cell carcinoma was slightly hyperechoic. Transpleural sonography identifies endobronchial tumour growth and bronchial wall destruction. With transthoracic sonography, the flooded animal lung can be completely

  9. Preconditioning allows engraftment of mouse and human embryonic lung cells, enabling lung repair in mice.

    PubMed

    Rosen, Chava; Shezen, Elias; Aronovich, Anna; Klionsky, Yael Zlotnikov; Yaakov, Yasmin; Assayag, Miri; Biton, Inbal Eti; Tal, Orna; Shakhar, Guy; Ben-Hur, Herzel; Shneider, David; Vaknin, Zvi; Sadan, Oscar; Evron, Shmuel; Freud, Enrique; Shoseyov, David; Wilschanski, Michael; Berkman, Neville; Fibbe, Willem E; Hagin, David; Hillel-Karniel, Carmit; Krentsis, Irit Milman; Bachar-Lustig, Esther; Reisner, Yair

    2015-08-01

    Repair of injured lungs represents a longstanding therapeutic challenge. We show that human and mouse embryonic lung tissue from the canalicular stage of development (20-22 weeks of gestation for humans, and embryonic day 15-16 (E15-E16) for mouse) are enriched with progenitors residing in distinct niches. On the basis of the marked analogy to progenitor niches in bone marrow (BM), we attempted strategies similar to BM transplantation, employing sublethal radiation to vacate lung progenitor niches and to reduce stem cell competition. Intravenous infusion of a single cell suspension of canalicular lung tissue from GFP-marked mice or human fetal donors into naphthalene-injured and irradiated syngeneic or SCID mice, respectively, induced marked long-term lung chimerism. Donor type structures or 'patches' contained epithelial, mesenchymal and endothelial cells. Transplantation of differentially labeled E16 mouse lung cells indicated that these patches were probably of clonal origin from the donor. Recipients of the single cell suspension transplant exhibited marked improvement in lung compliance and tissue damping reflecting the energy dissipation in the lung tissues. Our study provides proof of concept for lung reconstitution by canalicular-stage human lung cells after preconditioning of the pulmonary niche.

  10. Minimal mass size of a stable {sup 3}He cluster

    SciTech Connect

    Guardiola, R.; Navarro, J.

    2005-03-01

    The minimal number of {sup 3}He atoms required to form a bound cluster has been estimated by means of a diffusion Monte Carlo procedure within the fixed-node approximation. Several importance sampling wave functions have been employed in order to consider different shell-model configurations. The resulting upper bound for the minimal number is 32 atoms.

  11. Internal Magnus effects in superfluid sup 3 He- A

    SciTech Connect

    Salmelin, R.H.; Salomaa, M.M. ); Mineev, V.P.

    1989-08-21

    Orbital angular momentum of the coherently aligned Cooper pairs in superfluid {sup 3}He-{ital A} is encountered by an object immersed in the condensate. We evaluate the associated quasiparticle-scattering asymmetry experienced by a negative ion; this leads to a measureable, purely quantum-mechanical reactive force deflecting the ion's trajectory. Possible hydrodynamic Magnus effects are also discussed.

  12. {sup 3}He neutral current detectors at SNO

    SciTech Connect

    Elliott, S.R.; Browne, M.C.; Doe, P.J.

    1998-09-01

    The flux of solar neutrinos measured via charged and neutral current interactions can provide a model independent test of neutrino oscillations. Since the Sudbury Neutrino Observatory uses heavy water as a target, it has a large sensitivity to both interactions. A technique for observing the neutral current breakup of the deuteron using {sup 3}He proportional counters is described.

  13. The Momentum Distribution of Liquid 3He, Revisited

    NASA Astrophysics Data System (ADS)

    Sokol, Paul; Bryan, Matthew; Prisk, Timothy

    Liquid 3He is a system of fundamental importance to condensed matter physics because it is a prototypical example of a strongly interacting fermion system whose interactions are well known. Quantum Monte Carlo calculations predict that the atomic momentum distribution of liquid 3He contains a Fermi surface discontinuity and an average atomic kinetic energy in the range 12-13 K at saturated vapor pressure. A number of high-resolution neutron Compton scattering studies of liquid 33He have been described in the literature, with experimenters observing no Fermi surface discontinuity and obtaining kinetic energies in the range of 8-10 K. In this presentation, we reconsider measurements of the momentum distribution of liquid 3He taken at 500 mK under 0, 10, 15 bar of pressure [R.M. Dimeo et al Physica B 241-243, 952 (1998)]. We demonstrate that there is complete agreement between the experimental data and quantum Monte Carlo calculations when instrumental resolution and final state effect corrections are taken into account. We also consider the prospects for a direct observation of the Fermi surface discontinuity in liquid 3He using neutron Compton scattering. This research was supported by NSF Award DGE-1069091.

  14. Progress in Polarized 3He Ion Source at RCNP

    SciTech Connect

    Tanaka, M.; Takahashi, Y.; Shimoda, T.; Yasui, S.; Yosoi, M.; Takahisa, K.; Shimakura, N.; Plis, Yu. A.; Donets, E. D.

    2007-06-13

    A long history on the polarized 3He ion source developed at RCNP is presented. We started with an 'OPPIS' (Optical Pumping Polarized Ion Source) and later found the fundamental difficulties in the OPPIS. To overcome them an 'EPPIS' (Electron Pumping Polarized Ion Source) was proposed and its validity was experimentally proven. However, a serious technical disadvantage was also found in the EPPIS. To avoid this disadvantage we proposed a new concept, 'SEPIS' (Spin Exchange Polarized Ion Source), which uses an enhanced spin-exchange cross section theoretically expected at low 3He+ incident energies in the 3He+ + Rb system. Next, we describe the present status of the SEPIS development: construction of a bench test device allowing the measurements of not only the spin-exchange cross sections {sigma}se but also the electron capture cross sections {sigma}ec for the 3He+ + Rb system. The latest experimental data on {sigma}ec are presented and compared with other previous experimental data and the theoretical calculations.Finally, a design study of the SEPIS for practical use in nuclear (cyclotron) and particle physics (synchrotron) is shortly mentioned.

  15. Stability and Spectra of Small 3He-4He Clusters

    NASA Astrophysics Data System (ADS)

    Navarro, J.; Fantoni, S.; Guardiola, R.; Zuker, A.

    Diffusion Monte Carlo calculations have been systematically performed to analyze the stability of small mixed 3He-4He clusters, as well as their excitation spectra. The picture that emerges is that of systems with strong shell effects whose binding and excitation energies are essentially determined by the monopole properties of an effective Hamiltonian.

  16. Inhaled cellulosic and plastic fibers found in human lung tissue.

    PubMed

    Pauly, J L; Stegmeier, S J; Allaart, H A; Cheney, R T; Zhang, P J; Mayer, A G; Streck, R J

    1998-05-01

    We report the results of studies undertaken to determine whether inhaled plant (i.e., cellulosic; e.g., cotton) and plastic (e.g., polyester) fibers are present in human lungs and, if so, whether inhaled fibers are also present in human lung cancers. Specimens of lung cancer of different histological types and adjacent nonneoplastic lung tissue were obtained from patients undergoing a lung resection for removal of a tumor. With the protection of a laminar flow hood and safeguards to prevent contamination by extraneous fibers, fresh, nonfixed, and nonstained samples of lung tissue were compressed between two glass microscope slides. Specimens in these dual slide chambers were examined with a microscope configured to permit viewing with white light, fluorescent light, polarizing light, and phase-contrast illumination. Near-term fetal bovine lungs and nonlung human tumors were used as controls. In contrast to the observations of these control tissues, morphologically heterogeneous fibers were seen repetitively in freshly excised human lung tissue using polarized light. Inhaled fibers were present in 83% of nonneoplastic lung specimens (n = 67/81) and in 97% of malignant lung specimens (n = 32/33). Thus, of the 114 human lung specimens examined, fibers were observed in 99 (87%). Examination of histopathology slides of lung tissue with polarized light confirmed the presence of inhaled cellulosic and plastic fibers. Of 160 surgical histopathology lung tissue slides, 17 were selected for critical examination; of these, fibers were identified in 13 slides. The inhalation of mineral (e.g., asbestos) fibers has been described by many investigators; we believe, however, that this is the first report of inhaled nonmineral (e.g., plant and plastic) fibers. These bioresistant and biopersistent cellulosic and plastic fibers are candidate agents contributing to the risk of lung cancer.

  17. Relevance of particle-induced rat lung tumors for assessing lung carcinogenic hazard and human lung cancer risk.

    PubMed Central

    Mauderly, J L

    1997-01-01

    Rats and other rodents are exposed by inhalation to identify agents that might present hazards for lung cancer in humans exposed by inhalation. In some cases, the results are used in attempts to develop quantitative estimates of human lung cancer risk. This report reviews evidence for the usefulness of the rat for evaluation of lung cancer hazards from inhaled particles. With the exception of nickel sulfate, particulate agents thought to be human lung carcinogens cause lung tumors in rats exposed by inhalation. The rat is more sensitive to carcinogenesis from nonfibrous particles than mice or Syrian hamsters, which have both produced false negatives. However, rats differ from mice and nonhuman primates in both the pattern of particle retention in the lung and alveolar epithelial hyperplastic responses to chronic particle exposure. Present evidence warrants caution in extrapolation from the lung tumor response of rats to inhaled particles to human lung cancer hazard, and there is considerable uncertainty in estimating unit risks for humans from rat data. It seems appropriate to continue using rats in inhalation carcinogenesis assays of inhaled particles, but the upper limit of exposure concentrations must be set carefully to avoid false-positive results. A positive finding in both rats and mice would give greater confidence that an agent presents a carcinogenic hazard to man, and both rats and mice should be used if the agent is a gas or vapor. There is little justification for including Syrian hamsters in assays of the intrapulmonary carcinogenicity of inhaled agents. PMID:9400748

  18. Presence of multiple lesion types with vastly different microenvironments in C3HeB/FeJ mice following aerosol infection with Mycobacterium tuberculosis.

    PubMed

    Irwin, Scott M; Driver, Emily; Lyon, Edward; Schrupp, Christopher; Ryan, Gavin; Gonzalez-Juarrero, Mercedes; Basaraba, Randall J; Nuermberger, Eric L; Lenaerts, Anne J

    2015-06-01

    Cost-effective animal models that accurately reflect the pathological progression of pulmonary tuberculosis are needed to screen and evaluate novel tuberculosis drugs and drug regimens. Pulmonary disease in humans is characterized by a number of heterogeneous lesion types that reflect differences in cellular composition and organization, extent of encapsulation, and degree of caseous necrosis. C3HeB/FeJ mice have been increasingly used to model tuberculosis infection because they produce hypoxic, well-defined granulomas exhibiting caseous necrosis following aerosol infection with Mycobacterium tuberculosis. A comprehensive histopathological analysis revealed that C3HeB/FeJ mice develop three morphologically distinct lesion types in the lung that differ with respect to cellular composition, degree of immunopathology and control of bacterial replication. Mice displaying predominantly the fulminant necrotizing alveolitis lesion type had significantly higher pulmonary bacterial loads and displayed rapid and severe immunopathology characterized by increased mortality, highlighting the pathological role of an uncontrolled granulocytic response in the lung. Using a highly sensitive novel fluorescent acid-fast stain, we were able to visualize the spatial distribution and location of bacteria within each lesion type. Animal models that better reflect the heterogeneity of lesion types found in humans will permit more realistic modeling of drug penetration into solid caseous necrotic lesions and drug efficacy testing against metabolically distinct bacterial subpopulations. A more thorough understanding of the pathological progression of disease in C3HeB/FeJ mice could facilitate modulation of the immune response to produce the desired pathology, increasing the utility of this animal model.

  19. Symmetry protected topological superfluid (3)He-B.

    PubMed

    Mizushima, Takeshi; Tsutsumi, Yasumasa; Sato, Masatoshi; Machida, Kazushige

    2015-03-25

    Owing to the richness of symmetry and well-established knowledge of bulk superfluidity, the superfluid (3)He has offered a prototypical system to study intertwining of topology and symmetry. This article reviews recent progress in understanding the topological superfluidity of (3)He in a multifaceted manner, including symmetry considerations, the Jackiw-Rebbi's index theorem, and the quasiclassical theory. Special focus is placed on the symmetry protected topological superfuidity of the (3)He-B confined in a slab geometry. The (3)He-B under a magnetic field is separated to two different sub-phases: the symmetry protected topological phase and non-topological phase. The former phase is characterized by the existence of symmetry protected Majorana fermions. The topological phase transition between them is triggered by the spontaneous breaking of a hidden discrete symmetry. The critical field is quantitatively determined from the microscopic calculation that takes account of magnetic dipole interaction of the (3)He nucleus. It is also demonstrated that odd-frequency even-parity Cooper pair amplitudes are emergent in low-lying quasiparticles. The key ingredients, symmetry protected Majorana fermions and odd-frequency pairing, bring an important consequence that the coupling of the surface states to an applied field is prohibited by the hidden discrete symmetry, while the topological phase transition with the spontaneous symmetry breaking is accompanied by anomalous enhancement and anisotropic quantum criticality of surface spin susceptibility. We also illustrate common topological features between topological crystalline superconductors and symmetry protected topological superfluids, taking UPt3 and Rashba superconductors as examples.

  20. Quantitative Anatomy of the Growing Lungs in the Human Fetus.

    PubMed

    Szpinda, Michał; Siedlaczek, Waldemar; Szpinda, Anna; Woźniak, Alina; Mila-Kierzenkowska, Celestyna; Badura, Mateusz

    2015-01-01

    Using anatomical, digital, and statistical methods we examined the three-dimensional growth of the lungs in 67 human fetuses aged 16-25 weeks. The lung dimensions revealed no sex differences. The transverse and sagittal diameters and the base circumference were greater in the right lungs while the lengths of anterior and posterior margins and the lung height were greater in the left lungs. The best-fit curves for all the lung parameters were natural logarithmic models. The transverse-to-sagittal diameter ratio remained stable and averaged 0.56 ± 0.08 and 0.52 ± 0.08 for the right and left lungs, respectively. For the right and left lungs, the transverse diameter-to-height ratio significantly increased from 0.74 ± 0.09 to 0.92 ± 0.08 and from 0.56 ± 0.07 to 0.79 ± 0.09, respectively. The sagittal diameter-to-height ratio significantly increased from 1.41 ± 0.23 to 1.66 ± 0.18 in the right lung, and from 1.27 ± 0.17 to 1.48 ± 0.22 in the left lung. In the fetal lungs, their proportionate increase in transverse and sagittal diameters considerably accelerates with relation to the lung height. The lung dimensions in the fetus are relevant in the evaluation of the normative pulmonary growth and the diagnosis of pulmonary hypoplasia.

  1. Quantitative analysis of regional airways obstruction using dynamic hyperpolarized 3He MRI-preliminary results in children with cystic fibrosis.

    PubMed

    Koumellis, Panos; van Beek, Edwin J R; Woodhouse, Neil; Fichele, Stan; Swift, Andrew J; Paley, Martyn N J; Hill, Catherine; Taylor, Chris J; Wild, Jim M

    2005-09-01

    To investigate regional airways obstruction in patients with cystic fibrosis (CF) with quantitative analysis of dynamic hyperpolarized (HP) (3)He MRI. Dynamic radial projection MRI of HP (3)He gas was used to study respiratory dynamics in a group of eight children with CF. Signal kinetics in a total of seven regions of interest (ROIs; three in each lung, and one in the trachea) were compared with the results of spirometric pulmonary function tests (PFTs). The tracheal signal intensity was used as a form of "input function" to normalize for input flow effects. A pattern of low flow rate in the upper lobes was observed. When the flow measurements from the peripheral ROIs were averaged to obtain an index of flow in the peripheral lung, a good correlation was found (P = 3.74 x 10(-5)) with the forced expired volume in one second (FEV1). These results suggest that a quantitative measurement of localized airways obstruction in the early stages of CF may be obtained from dynamic (3)He MRI by using the slope of the signal rise as a measure of air flow into the peripheral lung. This study also demonstrates that children can cooperate well with the (3)He MRI technique. (c) 2005 Wiley-Liss, Inc.

  2. The effect of ex vivo lung perfusion on microbial load in human donor lungs.

    PubMed

    Andreasson, Anders; Karamanou, Danai M; Perry, John D; Perry, Audrey; Ӧzalp, Faruk; Butt, Tanveer; Morley, Katie E; Walden, Hannah R; Clark, Stephen C; Prabhu, Mahesh; Corris, Paul A; Gould, Kate; Fisher, Andrew J; Dark, John H

    2014-09-01

    Ex vivo lung perfusion (EVLP) has emerged as a technique to potentially recondition unusable donor lungs for transplantation. Beneficial effects of EVLP on physiologic function have been reported, but little is known about the effect of normothermic perfusion on the infectious burden of the donor lung. In this study, we investigated the effect of EVLP on the microbial load of human donor lungs. Lungs from 18 human donors considered unusable for transplantation underwent EVLP with a perfusate containing high-dose, empirical, broad-spectrum anti-microbial agents. Quantitative cultures of bacteria and fungi were performed on bronchoalveolar lavage fluid from the donor lung before and after 3 to 6 hours of perfusion. The identification of any organisms and changes in number of colony forming units before and after EVLP were assessed and anti-microbial susceptibilities identified. Thirteen out of 18 lungs had positive cultures, with bacterial loads significantly decreasing after EVLP. Yeast loads increased when no anti-fungal treatment was given, but were reduced when prophylactic anti-fungal treatment was added to the circuit. Six lungs were ultimately transplanted into patients, all of whom survived to hospital discharge. There was 1 death at 11 months. Our study shows that EVLP with high-dose, empirical anti-microbial agents in the perfusate is associated with an effective reduction in the microbial burden of the donor lung, a benefit that has not previously been demonstrated. Copyright © 2014 International Society for Heart and Lung Transplantation. Published by Elsevier Inc. All rights reserved.

  3. Overview of the n3He Experiment and Target Chamber

    NASA Astrophysics Data System (ADS)

    McCrea, Mark; n3He Collaboration

    2017-01-01

    The n3He Experiment aims to measure the parity-violating asymmetry in the direction of proton emission relative to the initial neutron polarization direction in the reaction n-> +3 He -> T + p + 765 keV to a high precision. The size of the asymmetry is estimated to be in the range - 9 . 5 - 2 . 5 ×10-8 , and our goal statistical accuracy is 2 ×10-8 . The experiment ran at the Spallation Neutron Source with data taking completing at the end of 2015. The experiment used a Helium-3 ionization chamber as the combined target and detector. Data analysis is underway and is currently in an advanced stage

  4. A D-3He IEC power unit for space applications

    NASA Astrophysics Data System (ADS)

    Miley, G. H.

    1999-01-01

    There is an urgent need for small power units for on-board and landing site power for space travel. The conceptual design of a 1-MWe Inertial Electrostatic Confinement (IEC) fusion unit burning D-3He fuel is considered here for such applications. The IEC is attractive for space power-its non-Maxwellian beam-beam character is well-suited for D-3He operation, small size units with a high specific power density are conceivable. Small scale IEC experiments have produced encouraging results and are used here as the basis for extrapolation to the space power unit. However, critical scale-up experiments are essential to verify the feasibility of the concept.

  5. Evidence for Split NMR Lines in Ferromagnetic 3He Films

    NASA Astrophysics Data System (ADS)

    Guo, Lei; Zhang, Jinshan; Du, Yuliang; Gould, C. M.; Bozler, H. M.

    2006-09-01

    In earlier experiments on ferromagnetic 3He films, we observed a complex lineshape due in part to the dipolar field generated by polarization of the 3He nuclei. Much of the complex lineshape can be explained by the known distribution of the Grafoil platelets. However, there remained some evidence for a split NMR line at some temperatures. In our new experiments on ZYX grade exfoliated graphite where the size of individual platelets is much larger and the angular distribution is three times smaller, this splitting has become more evident over a wider range of temperatures. Now it is clear that the complex lineshape includes two peaks along with remaining orientation effects. We also find that roughly 2% of our signal comes from randomly oriented platelets. We present the details of our model for analyzing these lineshapes and the experimental results for the line splitting at several coverages in the ferromagnetic range. We discuss the possible sources of this line splitting.

  6. Data acquisition system for the n3He experiment

    NASA Astrophysics Data System (ADS)

    Kabir, Latiful

    2014-03-01

    The n3He experiment at the Spallation Neutron Source will measure the parity violating spin asymmetry of the recoil proton in the reaction n+3He -->p +T +765 KeV. This is sensitive to ΔI = 0 and 1 components of the Hadronic Weak Interaction (HWI), and is expected to be extremely small (of the order 10-7) . Protons from the reaction are recorded in current mode in order to achieve a statistical sensitivity of 10-8 in a reasonable amount of time. In addition instrumental asymmetries must be suppressed by an additional order of magnitude. The asymmetry is measured as a function of time-of-flight of the neutron to study the energy dependence of any systematic effects. We will present details and preliminary tests of the 144 channel data acquisition system designed to meet these requirements.

  7. Acceleration of 3HE and heavy ions at interplanetary shocks

    NASA Astrophysics Data System (ADS)

    Desai, M. I.; Mason, G. M.; Dwyer, J. R.; Mazur, J. E.; Smith, C. W.; Koug, R. M.

    2001-08-01

    We have surveyed the 0.5-2.0 MeV nucleon-1 ion composition of 56 interplanetary shocks (IP) observed with the Ultra-Low-Energy Isotope Spectrometer (ULEIS) on board the Advanced Composition Explorer (ACE) from 1997 October 1 through 2000 November 30. Our results show the first ever measurement (25 cases) of 3 He ions being accelerated at IP shocks. The 3 He/4 He ratio at the 25 shocks exhibited a wide range of values between 0.00140.24; the ratios were enhanced between factors of ~3-600 over the solar wind value. During the survey period, the occurrence probability of 3 He-rich shocks increased with rising solar activity as measured in terms of the daily occurrence rates of sunspots and X-ray flares. The 3 He enhancements at IP shocks cannot be attributed to rigidity dependent acceleration of solar wind ions and are better explained if the shocks accelerate ions from multiple sources, one being remnant impulsive solar flare material enriched in 3 He ions. Our results also indicate that the contribution of impulsive flares to the seed population for IP shocks varies from event to event, and that the interplanetary medium is being replenished with impulsive material more frequently during periods of increased solar activity. 1. Introduction Enhancements in the intensities of energetic ions associated with transient interplanetary (IP) shocks have been observed routinely at 1 AU since the 1960's (e.g., Reames 1999). It is presently believed that the majority of such IP shocks are driven by fast coronal mass ejections or CMEs as they propagate through interplanetary space (e.g., Gosling 1993), and that the associated ion intensity enhancements are due to diffusive shock acceleration of solar wind ions (Lee 1983; Jones and Ellison 1991; Reames 1999). However, the putative solar wind origin of the IP-shock accelerated ions is based on composition measurements associated with a very limited number of individual IP shocks (Klecker et al. 1981; Hovestadt et al. 1982; Tan et

  8. Proton polarization from π+ absorption in 3He

    NASA Astrophysics Data System (ADS)

    Maytal-Beck, S.; Aclander, J.; Altman, A.; Ashery, D.; Hahn, H.; Moinester, M. A.; Rahav, A.; Feltham, A.; Jones, G.; Pavan, M.; Sevior, M.; Hutcheon, D.; Ottewell, D.; Smith, G. R.; Niskanen, J. A.

    1992-05-01

    We present the first polarization measurements for pion absorption on a nucleus heavier than the deuteron. The polarization of protons resulting from π+ absorption in the 3He was measured at bombarding energies of 120 and 250 MeV. Protons from absorption in a quasideuteron were selected by applying kinematical constraints. A significant discrepancy was observed between the experimental results and theoretical predictions. At 120 MeV the measured polarizations for 3He are consistent with those of the deuteron. At 250 MeV the angular distribution of the polarization is significantly different than for the deuteron, showing sensitivity to the nuclear density, and thus may be sensitive to short range correlations between nucleons.

  9. Spectroscopic quantitation of cytochrome P-450 in human lung microsomes.

    PubMed

    Wheeler, C W; Guenthner, T M

    1990-01-01

    The cytochrome P-450 content of human lung microsomes was measured by difference spectroscopy of the carbon monoxide-complexed hemoprotein. These measurements were only possible after the microsome preparation had been subjected to centrifugation over a discontinuous sucrose gradient, to remove an opaque black contaminant. The specific concentration of total cytochrome P-450 in human lung microsomes is essentially identical to that of microsomes prepared under identical conditions from untreated baboon lungs, but is only 0.7% of the specific content found in lung microsomes from untreated rabbits. These measurements correspond well to the observed metabolic capacities of the various microsome samples.

  10. Quantification of Trapped Gas with CT and 3He MR Imaging in a Porcine Model of Isolated Airway Obstruction1

    PubMed Central

    Salito, Caterina; Aliverti, Andrea; Gierada, David S.; Deslée, Gaetan; Pierce, Richard A.; Macklem, Peter T.; Woods, Jason C.

    2009-01-01

    Purpose: To quantify regional gas trapping in the lung by using computed tomographic (CT)–determined specific gas volume and hyperpolarized helium 3 (3He) magnetic resonance (MR) imaging in a porcine model of airway obstruction. Materials and Methods: Four porcine lungs were removed after sacrifice for unrelated cardiac experiments, for which animal studies approval was obtained. Dynamic expiratory thin-section CT and 3He MR imaging were performed during passive deflation from total lung capacity after obstructions were created with inverted one-way endobronchial exit valves in segmental or lobar bronchi to produce identifiable regions of trapped gas. Changes in specific gas volume were assessed from CT data for defined regions of interest within and outside of obstructed segments and for entire lobes. Helium 3 data were analyzed according to the corresponding regional signal reduction during expiration, compared with the total magnetic moment at each time point. Results: In 4.5 seconds of free collapse, volume decreased by 6% ± 2 (standard error) and 53% ± 3, respectively, in trapped-gas lobes and in unobstructed regions (P < .0001). Specific gas volume changed by 6% ± 2 in areas of trapped gas and decreased by 56% ± 3 in unobstructed regions, from 3.4 mL/g ± 0.2 to 1.5 mL/g ± 0.1 (P < .0001). The 3He signal intensity decreased by 25% ± 6 and 71% ± 3, respectively, in trapped-gas and normal regions (P = .0008). In unobstructed regions, the percentage decreases in specific gas volume and 3He signal intensity were not statistically different from one another (P = .89). Conclusion: The results obtained from the model of gas trapping demonstrate that CT-determined specific gas volume and 3He MR imaging can help identify and quantify the extent of regional trapped gas in explanted porcine lungs. © RSNA, 2009 PMID:19703847

  11. Thermal Conductivity of Spin-Polarized Liquid {sup 3}He

    SciTech Connect

    Sawkey, D.; Puech, L.; Wolf, P.E.

    2006-06-02

    We present the first measurements of the thermal conductivity of spin-polarized normal liquid {sup 3}He. Using the rapid melting technique to produce nuclear polarizations up to 0.7, and a vibrating wire both as a heater and a thermometer, we show that, unlike the viscosity, the conductivity increases much less than predicted for s-wave scattering. We suggest that this might be due to a small probability for head-on collisions between quasiparticles.

  12. NOVEL CONCEPTS FOR ISOTOPIC SEPARATION OF 3HE/4HE

    SciTech Connect

    Roy, L.; Nigg, H.; Watson, H.

    2012-09-04

    The research outlined below established theoretical proof-of-concept using ab initio calculations that {sup 3}He can be separated from {sup 4}He by taking advantage of weak van der Waals interactions with other higher molecular weight rare gases such as xenon. To the best of our knowledge, this is the only suggested method that exploits the physical differences of the isotopes using a chemical interaction.

  13. Hard Two-body Photodisintegration of ^3He

    SciTech Connect

    Pomerantz, Ishay Ari; Ilieva, Yordanka Yordanova; Gilman, Ronald; Higinbotham, Douglas W.; Piasetzky, Eliazer Israel; Strauch, Steffen

    2013-06-01

    We have measured cross sections for the {gamma}+{sup 3}He->p+d reaction at photon energies of 0.4 - 1.4 GeV and a center-of-mass angle of 90 deg. We observe dimensional scaling above 0.7 GeV at this center-of-mass angle. This is the first observation of dimensional scaling in the photodisintegration of a nucleus heavier than the deuteron.

  14. Hard two-body photodisintegration of 3He.

    PubMed

    Pomerantz, I; Ilieva, Y; Gilman, R; Higinbotham, D W; Piasetzky, E; Strauch, S; Adhikari, K P; Aghasyan, M; Allada, K; Amaryan, M J; Anefalos Pereira, S; Anghinolfi, M; Baghdasaryan, H; Ball, J; Baltzell, N A; Battaglieri, M; Batourine, V; Beck, A; Beck, S; Bedlinskiy, I; Berman, B L; Biselli, A S; Boeglin, W; Bono, J; Bookwalter, C; Boiarinov, S; Briscoe, W J; Brooks, W K; Bubis, N; Burkert, V; Camsonne, A; Canan, M; Carman, D S; Celentano, A; Chandavar, S; Charles, G; Chirapatpimol, K; Cisbani, E; Cole, P L; Contalbrigo, M; Crede, V; Cusanno, F; D'Angelo, A; Daniel, A; Dashyan, N; de Jager, C W; De Vita, R; De Sanctis, E; Deur, A; Djalali, C; Dodge, G E; Doughty, D; Dupre, R; Dutta, C; Egiyan, H; El Alaoui, A; El Fassi, L; Eugenio, P; Fedotov, G; Fegan, S; Fleming, J A; Fradi, A; Garibaldi, F; Geagla, O; Gevorgyan, N; Giovanetti, K L; Girod, F X; Glister, J; Goetz, J T; Gohn, W; Golovatch, E; Gothe, R W; Griffioen, K A; Guegan, B; Guidal, M; Guo, L; Hafidi, K; Hakobyan, H; Harrison, N; Heddle, D; Hicks, K; Ho, D; Holtrop, M; Hyde, C E; Ireland, D G; Ishkhanov, B S; Isupov, E L; Jiang, X; Jo, H S; Joo, K; Katramatou, A T; Keller, D; Khandaker, M; Khetarpal, P; Khrosinkova, E; Kim, A; Kim, W; Klein, F J; Koirala, S; Kubarovsky, A; Kubarovsky, V; Kuleshov, S V; Kvaltine, N D; Lee, B; LeRose, J J; Lewis, S; Lindgren, R; Livingston, K; Lu, H Y; MacGregor, I J D; Mao, Y; Martinez, D; Mayer, M; McCullough, E; McKinnon, B; Meekins, D; Meyer, C A; Michaels, R; Mineeva, T; Mirazita, M; Moffit, B; Mokeev, V; Montgomery, R A; Moutarde, H; Munevar, E; Munoz Camacho, C; Nadel-Turonski, P; Nasseripour, R; Nepali, C S; Niccolai, S; Niculescu, G; Niculescu, I; Osipenko, M; Ostrovidov, A I; Pappalardo, L L; Paremuzyan, R; Park, K; Park, S; Petratos, G G; Phelps, E; Pisano, S; Pogorelko, O; Pozdniakov, S; Procureur, S; Protopopescu, D; Puckett, A J R; Qian, X; Qiang, Y; Ricco, G; Rimal, D; Ripani, M; Ritchie, B G; Rodriguez, I; Ron, G; Rosner, G; Rossi, P; Sabatié, F; Saha, A; Saini, M S; Sarty, A J; Sawatzky, B; Saylor, N A; Schott, D; Schulte, E; Schumacher, R A; Seder, E; Seraydaryan, H; Shneor, R; Smith, G D; Sokhan, D; Sparveris, N; Stepanyan, S S; Stepanyan, S; Stoler, P; Subedi, R; Sulkosky, V; Taiuti, M; Tang, W; Taylor, C E; Tkachenko, S; Ungaro, M; Vernarsky, B; Vineyard, M F; Voskanyan, H; Voutier, E; Walford, N K; Wang, Y; Watts, D P; Weinstein, L B; Weygand, D P; Wojtsekhowski, B; Wood, M H; Yan, X; Yao, H; Zachariou, N; Zhan, X; Zhang, J; Zhao, Z W; Zheng, X; Zonta, I

    2013-06-14

    We have measured cross sections for the γ(3)He → pd reaction at photon energies of 0.4-1.4 GeV and a center-of-mass angle of 90°. We observe dimensional scaling above 0.7 GeV at this center-of-mass angle. This is the first observation of dimensional scaling in the photodisintegration of a nucleus heavier than the deuteron.

  15. Internal Magnus effects in superfluid 3He-A

    NASA Astrophysics Data System (ADS)

    Salmelin, R. H.; Salomaa, M. M.; Mineev, V. P.

    The orbital angular momentum of the coherently aligned Cooper pairs in superfluid (3)He-A is transmitted to an object immersed in the condensate. The authors evaluate the quasiparticle-scattering asymmetry experienced by a negative ion; this leads to a measurable, purely quantum-mechanical Magnus force deflecting the ion's trajectory. Close to T(sub c), possible hydrodynamic Magnus effects are smaller by the factor delta sub A/(k sub B)(T sub c).

  16. Perturbed gap functions in superfluid /sup 3/He

    SciTech Connect

    McInerney, M.F.

    1980-12-01

    New forms of the Balian-Werthamer (BW) and of the Anderson-Brinkman--Morel (ABM) gap solutions for superfluid /sup 3/He are found in the presence of perturbations. Using the perturbed form of the BW solution, a discrepancy is found between the static and dynamic magnetic susceptibilities, which is shown to be in reasonable agreement with the experimental measurements of Sager and Webb. A self-consistent diagrammatic Green's function approach is used.

  17. Production and Assessment of Decellularized Pig and Human Lung Scaffolds

    PubMed Central

    Niles, Jean; Riddle, Michael; Vargas, Gracie; Schilagard, Tuya; Ma, Liang; Edward, Kert; La Francesca, Saverio; Sakamoto, Jason; Vega, Stephanie; Ogadegbe, Marie; Mlcak, Ronald; Deyo, Donald; Woodson, Lee; McQuitty, Christopher; Lick, Scott; Beckles, Daniel; Melo, Esther; Cortiella, Joaquin

    2013-01-01

    The authors have previously shown that acellular (AC) trachea-lung scaffolds can (1) be produced from natural rat lungs, (2) retain critical components of the extracellular matrix (ECM) such as collagen-1 and elastin, and (3) be used to produce lung tissue after recellularization with murine embryonic stem cells. The aim of this study was to produce large (porcine or human) AC lung scaffolds to determine the feasibility of producing scaffolds with potential clinical applicability. We report here the first attempt to produce AC pig or human trachea-lung scaffold. Using a combination of freezing and sodium dodecyl sulfate washes, pig trachea-lungs and human trachea-lungs were decellularized. Once decellularization was complete we evaluated the structural integrity of the AC lung scaffolds using bronchoscopy, multiphoton microscopy (MPM), assessment of the ECM utilizing immunocytochemistry and evaluation of mechanics through the use of pulmonary function tests (PFTs). Immunocytochemistry indicated that there was loss of collagen type IV and laminin in the AC lung scaffold, but retention of collagen-1, elastin, and fibronectin in some regions. MPM scoring was also used to examine the AC lung scaffold ECM structure and to evaluate the amount of collagen I in normal and AC lung. MPM was used to examine the physical arrangement of collagen-1 and elastin in the pleura, distal lung, lung borders, and trachea or bronchi. MPM and bronchoscopy of trachea and lung tissues showed that no cells or cell debris remained in the AC scaffolds. PFT measurements of the trachea-lungs showed no relevant differences in peak pressure, dynamic or static compliance, and a nonrestricted flow pattern in AC compared to normal lungs. Although there were changes in content of collagen I and elastin this did not affect the mechanics of lung function as evidenced by normal PFT values. When repopulated with a variety of stem or adult cells including human adult primary alveolar epithelial type II

  18. Resonant quasiparticle-ion scattering in anisotropic superfluid 3He

    NASA Astrophysics Data System (ADS)

    Salmelin, R. H.; Salomaa, M. M.

    1990-03-01

    Low-energy excitations in quantum fluids are most directly encountered by ions. In the superfluid phases of 3He the relevant elementary excitations are Bogoliubov quasiparticles, which undergo repeated scattering off an ion in the presence of a divergent density of states. We present a quantum-mechanical calculation of the resonant 3He quasiparticle-scattering-limited mobility for negative ions in the anisotropic bulk 3A (A phase) and 3P (polar phase) that is exact when the quasiparticles scatter elastically. We develop a numerical scheme to solve the singular equations for quasiparticle-ion scattering in the A and P phases. Both of these superfluid phases feature a uniaxially symmetric order parameter but distinct topology for the magnitude of the energy gap on the Fermi sphere, i.e., points versus lines of nodes. In particular, the perpetual orbital circulation of Cooper pairs in 3A results in a novel, purely quantum-mechanical intrinsic Magnus effect, which is absent in the polar phase, where Cooper pairs possess no spontaneous orbital angular momentum. This is of interest also for transport properties of heavy-fermion superconductors. We discuss the 3He quasiparticle-ion cross sections, which allow one to account for the mobility data with essentially no free parameters. The calculated mobility thus facilitates an introduction of ``ion spectroscopy'' to extract useful information on fundamental properties of the superfluid state, such as the temperature dependence of the energy gap in 3A.

  19. Incorporating metal into polarized 3He target cells

    NASA Astrophysics Data System (ADS)

    Katugampola, Sumudu K.; Matyas, Daniel J.; Wang, Yunxiao; Tobias, William A.; Nelyubin, Vladimir; Cates, Gordon D.

    2017-01-01

    An upcoming measurement at Jefferson Laboratory (JLab) of the electric form factor of the neutron will utilize a polarized 3He target at high luminosity. While polarized 3He targets at JLab have previously been made entirely of glass, we describe progress toward incorporating metal windows for the electron beam. Under the conditions of our targets, very few studies have been done on the spin-relaxation of nuclear-polarized 3He on metal surfaces. We have found good performance by using Oxygen Free High Conductivity (OFHC) copper substrates electroplated with gold. The glass-to-metal transitions within our test cells were based on Housekeeper seals. We have further established that Uranium glass (Canary glass) has excellent spin-relaxation properties, and can serve as a transition glass from Pyrex to Aluminosilicate glass (GE180). Another finding was that spin-relaxation properties were sensitive to the manner in which cells were annealed, an important issue because of constraints when annealing cells containing both metal and glass.

  20. Probing the Faraday Effect of Polarized ^3He

    NASA Astrophysics Data System (ADS)

    Phelps, Gretchen; Abney, Josh; Korsch, Wolfgang

    2013-04-01

    The Faraday Effect refers to the phenomenon in which the polarization of light transmitted through a magnetized medium is rotated. The relation φ=VlB describes the magnitude of the rotation, where V is the material dependent Verdet constant and l is the length of the medium in an applied magnetic field B. Polarized ^3He, generated in a glass cell constructed of GE-180, gives rise to a Faraday rotation via nuclear spin optical rotation (NSOR), a measure of which establishes a new technique in ^3He polarization monitoring. Our set-up incorporates a triple-modulation technique with present sensitivities at the μrad level. This is accomplished through the combination of a photo-elastic modulator, an optical chopper, and a sinusoidally driven magnetic field. Several calibration samples were used to test the triple-modulation method. Good agreement between our results and the commonly accepted values for the Verdet constant was achieved. Technical challenges and progress towards the determination of V^3He will be presented.

  1. Chiral Phases of Superfluid 3He in an Anisotropic Medium

    NASA Astrophysics Data System (ADS)

    Sauls, James

    2013-03-01

    I report theoretical results for the phases of superfluid 3He infused into homogeneous uniaxial aerogel. Ginzburg-Landau (GL) theory for a class of equal-spin-pairing (ESP) states in a medium with uniaxial anisotropy is developed and used to analyze recent experiments on uniaxially strained aerogels. For 3He in an axially ``stretched'' aerogel GL theory predicts a transition from normal liquid into a chiral ABM phase in which the chirality axis is aligned along the strain axis. This state is protected from random fluctuations in the anisotropy direction, has a positive NMR shift, a sharp NMR resonance line and is in quantitative agreement with NMR in the high-temperature ESP-1 phase of superfluid 3He in axially stretched aerogel. A second transition into a bi-axial phase is predicted to onset at a slightly lower temperature. This phase is an ESP state, breaks time-reversal symmetry, and is defined by an order parameter that spontaneously breaks axial rotation symmetry. The bi-axial phase has a continuous degeneracy associated with broken axial symmetry. Theoretical predictions for the NMR frequency shifts provide an identification of the ESP-2 phase as the bi-axial state, partially disordered by random anisotropy (Larkin-Imry-Ma effect). Supported by National Science Foundation Grant DMR-1106315.

  2. Uranium Neutron Coincidence Collar Model Utilizing 3He

    SciTech Connect

    Siciliano, Edward R.; Rogers, Jeremy L.; Schweppe, John E.; Lintereur, Azaree T.; Kouzes, Richard T.

    2012-07-30

    The Department of Energy Office of Nuclear Safeguards (NA-241) is supporting the project 'Coincidence Counting With Boron-Based Alternative Neutron Detection Technology' at Pacific Northwest National Laboratory (PNNL) for development of an alternative neutron coincidence counter. The goal of this project is to design, build and demonstrate a boron-lined proportional tube based alternative system in a configuration typically used for 3He-based coincidence counter applications. The specific application selected for boron-lined tube replacement in this project was one of the Uranium Neutron Coincidence Collar (UNCL) designs. This report, providing results for model development of a UNCL, is a deliverable under Task 2 of the project. The current UNCL instruments utilize 3He tubes. As the first step in developing and optimizing a boron-lined proportional counter based version of the UNCL, models of eight different 3He-based UNCL detectors currently in use were developed and evaluated. A comparison was made between the simulated results and measured efficiencies for those systems with values reported in the literature. The reported experimental measurements for efficiencies and die-away times agree to within 10%.

  3. High Efficiency Spin Flipper for the n3He Experiment

    NASA Astrophysics Data System (ADS)

    Hayes, Christopher; n3He Collaboration

    2015-10-01

    The n3He experiment, constructed on the Fundamental Neutron Physics Beamline (FnPB) at the Spallation Neutron Source, is designed to measure the parity violating (PV) proton asymmetry Ap in the capture reaction n +3 He -->3 H + p + 765 keV The asymmetry has an estimated value Ap ~ - 1 ×10-7 and is directly related to the weak isospin conserved couplings hρ0 and ωρ0 which are of fundamental interest in the verification of the meson exchange model of low energy NN intereactions. Data production for the n3He experiment began in February 2015 and is scheduled to continue thru December 2015 - reaching a statistical sensitivity δAp ~10-8 or better. I will discuss the spin flipper which is designed using the theory of double cosine-theta coils, and capable of flipping neutron spins with an efficiency approaching its maximum value ɛsf = 1 . I will also discuss the theory of Spin Magnetic Resonance (SMR) and how it is employed by the spin flipper to flip 60 Hz pulses of cold neutrons over a range of wavelengths.

  4. Human sweat metabolomics for lung cancer screening.

    PubMed

    Calderón-Santiago, Mónica; Priego-Capote, Feliciano; Turck, Natacha; Robin, Xavier; Jurado-Gámez, Bernabé; Sanchez, Jean C; Luque de Castro, María D

    2015-07-01

    Sweat is one of the less employed biofluids for discovery of markers in spite of its increased application in medicine for detection of drugs or for diagnostic of cystic fibrosis. In this research, human sweat was used as clinical sample to develop a screening tool for lung cancer, which is the carcinogenic disease with the highest mortality rate owing to the advanced stage at which it is usually detected. In this context, a method based on the metabolite analysis of sweat to discriminate between patients with lung cancer versus smokers as control individuals is proposed. The capability of the metabolites identified in sweat to discriminate between both groups of individuals was studied and, among them, a trisaccharide phosphate presented the best independent performance in terms of the specificity/sensitivity pair (80 and 72.7%, respectively). Additionally, two panels of metabolites were configured using the PanelomiX tool as an attempt to reduce false negatives (at least 80% specificity) and false positives (at least 80% sensitivity). The first panel (80% specificity and 69% sensitivity) was composed by suberic acid, a tetrahexose, and a trihexose, while the second panel (69% specificity and 80% sensitivity) included nonanedioic acid, a trihexose, and the monoglyceride MG(22:2). Thus, the combination of the five metabolites led to a single panel providing 80% specificity and 79% sensitivity, reducing the false positive and negative rates to almost 20%. The method was validated by estimation of within-day and between-days variability of the quantitative analysis of the five metabolites.

  5. Human embryonic stem cells and lung regeneration.

    PubMed

    Varanou, A; Page, C P; Minger, S L

    2008-10-01

    Human embryonic stem cells are pluripotent cells derived from the inner cell mass of preimplantation stage embryos. Their unique potential to give rise to all differentiated cell types has generated great interest in stem cell research and the potential that it may have in developmental biology, medicine and pharmacology. The main focus of stem cell research has been on cell therapy for pathological conditions with no current methods of treatment, such as neurodegenerative diseases, cardiac pathology, retinal dysfunction and lung and liver disease. The overall aim is to develop methods of application either of pure cell populations or of whole tissue parts to the diseased organ under investigation. In the field of pulmonary research, studies using human embryonic stem cells have succeeded in generating enriched cultures of type II pneumocytes in vitro. On account of their potential of indefinite proliferation in vitro, embryonic stem cells could be a source of an unlimited supply of cells available for transplantation and for use in gene therapy. Uncovering the ability to generate such cell types will expand our understanding of biological processes to such a degree that disease understanding and management could change dramatically.

  6. DETECTION AND QUANTITATION OF FALLOUT PARTICLES IN A HUMAN LUNG.

    PubMed

    WEGST, A V; PELLETIER, C A; WHIPPLE, G H

    1964-02-28

    Portions of an adult human lung were studied by autoradiography in order to detect the presence of fallout particles. The radioactivity in the remainder of the tissue was determined with a gamma-ray spectrometer. Four particles were found and their activities were determined. From the measurement for total-fission-product activity in the lung tissue it was calculated that there were approximately 264 particles in the right lung at the time of death.

  7. Effects of Diffusion Time on Short-Range Hyperpolarized 3He Diffusivity Measurements in Emphysema

    SciTech Connect

    Gierada, David S.; Woods, Jason C.; Bierhals, Andrew J.; Bartel, Seth T.; Ritter, Jon H.; Choong, Cliff K.; Das, Nitin A.; Hong, Cheng; Pilgram, Thomas K.; Chang, Yulin V.; Jacob, Rick E.; Hogg, James C.; Battafarano, Richard J.; Cooper, Joel D.; Meyers, Bryan F.; Patterson, G Alexander; Yablonskiy, Dmitriy A.; Conradi, Mark S.

    2009-09-28

    Purpose: To characterize the effect of diffusion time on short-range hyperpolarized 3He MR diffusion measurements across a wide range of emphysema severity. Materials and Methods: 3He diffusion MR imaging was performed on 19 lungs or lobes resected from 18 subjects with varying degrees of emphysema using 3 diffusion times (1.6 msec, 5 msec, and 10 msec) at constant b value. Emphysema severity was quantified as the mean apparent diffusion coefficient (ADC) and as the percentage of pixels with ADC higher than multiple thresholds from 0.30-0.55 cm2/sec (ADC index). Quantitative histology (mean linear intercept) was obtained in 10 of the lung specimens from 10 of the subjects. Results: The mean ADCs with diffusion times of 1.6, 5.0, and 10.0 msec were 0.46, 0.40, and 0.37 cm2/sec, respectively (P <0.0001, ANOVA). There was no relationship between the ADC magnitude and the effect of diffusion time on ADC values. Mean linear intercept correlated with ADC (r=0.91-0.94, P<0.001) and ADC index (r=0.78-0.92, P<0.01) at all diffusion times.

  8. Effects of Diffusion Time on Short-Range Hyperpolarized 3He Diffusivity Measurements in Emphysema

    PubMed Central

    Gierada, David S.; Woods, Jason C.; Bierhals, Andrew J.; Bartel, Seth T.; Ritter, Jon H.; Choong, Cliff K.; Das, Nitin A.; Hong, Cheng; Pilgram, Thomas K.; Chang, Yulin V.; Jacob, Richard E.; Hogg, James C.; Battafarano, Richard J.; Cooper, Joel D.; Meyers, Bryan F.; Patterson, G. Alexander; Yablonskiy, Dmitriy A.; Conradi, Mark S.

    2010-01-01

    Purpose To characterize the effect of diffusion time on short-range hyperpolarized 3He MR diffusion measurements across a wide range of emphysema severity. Materials and Methods 3He diffusion MR imaging was performed on 19 lungs or lobes resected from 18 subjects with varying degrees of emphysema using 3 diffusion times (1.6 msec, 5 msec, and 10 msec) at constant b value. Emphysema severity was quantified as the mean apparent diffusion coefficient (ADC) and as the percentage of pixels with ADC higher than multiple thresholds from 0.30–0.55 cm2/sec (ADC index). Quantitative histology (mean linear intercept) was obtained in 10 of the lung specimens from 10 of the subjects. Results The mean ADCs with diffusion times of 1.6, 5.0, and 10.0 msec were 0.46, 0.40, and 0.37 cm2/sec, respectively (P <0.0001, ANOVA). There was no relationship between the ADC magnitude and the effect of diffusion time on ADC values. Mean linear intercept correlated with ADC (r=0.91–0.94, P<0.001) and ADC index (r=0.78–0.92, P<0.01) at all diffusion times. Conclusion Decreases in ADC with longer diffusion time were unrelated to emphysema severity. The strong correlations between the ADC at all diffusion times tested and quantitative histology demonstrate that the ADC is a robust measure of emphysema. PMID:19787725

  9. Two and three-dimensional segmentation of hyperpolarized 3He magnetic resonance imaging of pulmonary gas distribution

    NASA Astrophysics Data System (ADS)

    Heydarian, Mohammadreza; Kirby, Miranda; Wheatley, Andrew; Fenster, Aaron; Parraga, Grace

    2012-03-01

    A semi-automated method for generating hyperpolarized helium-3 (3He) measurements of individual slice (2D) or whole lung (3D) gas distribution was developed. 3He MRI functional images were segmented using two-dimensional (2D) and three-dimensional (3D) hierarchical K-means clustering of the 3He MRI signal and in addition a seeded region-growing algorithm was employed for segmentation of the 1H MRI thoracic cavity volume. 3He MRI pulmonary function measurements were generated following two-dimensional landmark-based non-rigid registration of the 3He and 1H pulmonary images. We applied this method to MRI of healthy subjects and subjects with chronic obstructive lung disease (COPD). The results of hierarchical K-means 2D and 3D segmentation were compared to an expert observer's manual segmentation results using linear regression, Pearson correlations and the Dice similarity coefficient. 2D hierarchical K-means segmentation of ventilation volume (VV) and ventilation defect volume (VDV) was strongly and significantly correlated with manual measurements (VV: r=0.98, p<.0001 VDV: r=0.97, p<.0001) and mean Dice coefficients were greater than 92% for all subjects. 3D hierarchical K-means segmentation of VV and VDV was also strongly and significantly correlated with manual measurements (VV: r=0.98, p<.0001 VDV: r=0.64, p<.0001) and the mean Dice coefficients were greater than 91% for all subjects. Both 2D and 3D semi-automated segmentation of 3He MRI gas distribution provides a way to generate novel pulmonary function measurements.

  10. A human breathing lung-on-a-chip.

    PubMed

    Huh, Dongeun Dan

    2015-03-01

    Here we describe a microphysiological system that replicates the functional unit of the living human lung. This human "breathing lung-on-a-chip" microdevice provides unique capabilities to reconstitute three-dimensional microarchitecture, dynamic mechanical activity, and integrated physiological function of the alveolar-capillary interface. We demonstrate the potential of this microengineered biomimetic model for screening environmental particulates and modeling complex human disease processes.

  11. Regional differences in alveolar density in the human lung are related to lung height.

    PubMed

    McDonough, John E; Knudsen, Lars; Wright, Alexander C; Elliott, W Mark; Ochs, Matthias; Hogg, James C

    2015-06-01

    The gravity-dependent pleural pressure gradient within the thorax produces regional differences in lung inflation that have a profound effect on the distribution of ventilation within the lung. This study examines the hypothesis that gravitationally induced differences in stress within the thorax also influence alveolar density in terms of the number of alveoli contained per unit volume of lung. To test this hypothesis, we measured the number of alveoli within known volumes of lung located at regular intervals between the apex and base of four normal adult human lungs that were rapidly frozen at a constant transpulmonary pressure, and used microcomputed tomographic imaging to measure alveolar density (number alveoli/mm3) at regular intervals between the lung apex and base. These results show that at total lung capacity, alveolar density in the lung apex is 31.6 ± 3.4 alveoli/mm3, with 15 ± 6% of parenchymal tissue consisting of alveolar duct. The base of the lung had an alveolar density of 21.2 ± 1.6 alveoli/mm3 and alveolar duct volume fraction of 29 ± 6%. The difference in alveolar density can be negated by factoring in the effects of alveolar compression due to the pleural pressure gradient at the base of the lung in vivo and at functional residual capacity.

  12. Comparative decellularization and recellularization of normal versus emphysematous human lungs.

    PubMed

    Wagner, Darcy E; Bonenfant, Nicholas R; Parsons, Charles S; Sokocevic, Dino; Brooks, Elice M; Borg, Zachary D; Lathrop, Melissa J; Wallis, John D; Daly, Amanda B; Lam, Ying Wai; Deng, Bin; DeSarno, Michael J; Ashikaga, Takamaru; Loi, Roberto; Weiss, Daniel J

    2014-03-01

    Acellular whole human lung scaffolds represent a unique opportunity for ex vivo tissue engineering. However, it remains unclear whether lungs from individuals with chronic lung diseases such as chronic obstructive pulmonary disease (COPD) can be appropriately decellularized and recellularized. To assess this, cadaveric human lungs from normal (non-smoking) patients and from patients with COPD (smoking history) were decellularized and found by histochemical and immunohistochemical staining, electron microscopy, and mass spectrometry to retain characteristic histological architecture and extracellular matrix components (ECM) reflecting either normal or COPD, particularly emphysematous, origin. Inoculation of human bronchial epithelial cells, endothelial progenitor cells, bone marrow-derived mesenchymal stem cells, and lung fibroblasts via airway or vascular routes into small, excised segments of the decellularized lungs demonstrated that normal lung scaffolds robustly supported initial engraftment and growth of each cell type for up to one month. In contrast, despite initial binding, all cell types inoculated into decellularized emphysematous lungs did not survive beyond one week. However, cell attachment and proliferation on solubilized ECM homogenates of decellularized normal and emphysematous lungs coated onto tissue culture plates was comparable and not impaired, suggesting that the 3-dimensional decellularized emphysematous scaffolds may lack the necessary ECM architecture to support sustained cell growth. Copyright © 2014 Elsevier Ltd. All rights reserved.

  13. Comparative Decellularization and Recellularization of Normal versus Emphysematous Human Lungs

    PubMed Central

    Wagner, Darcy. E.; Bonenfant, Nicholas. R.; Parsons, Charles; Sokocevic, Dino; Brooks, Elice. M.; Borg, Zachary. D.; Lathrop, Melissa. J.; Wallis, John. D.; Daly, Amanda. B.; Lam, Ying Wai; Deng, Bin; DeSarno, Michael. J.; Ashikaga, Takamaru; Loi, Roberto; Weiss, Daniel. J.

    2014-01-01

    Acellular whole human lung scaffolds represent a unique opportunity for ex vivo tissue engineering. However, it remains unclear whether lungs from individuals with chronic lung diseases such as chronic obstructive pulmonary disease (COPD) can be appropriately decellularized and recellularized. To assess this, cadaveric human lungs from normal (non-smoking) patients and from patients with COPD (smoking history) were decellularized and found by histochemical and immunohistochemical staining, electron microscopy, and mass spectrometry to retain characteristic histological architecture and extracellular matrix components (ECM) reflecting either normal or COPD, particularly emphysematous, origin. Inoculation of human bronchial epithelial cells, endothelial progenitor cells, bone marrow-derived mesenchymal stem cells, and lung fibroblasts via airway or vascular routes into small, excised segments of the decellularized lungs demonstrated that normal lung scaffolds robustly supported initial engraftment and growth of each cell type for up to one month. In contrast, despite initial binding, all cell types inoculated into decellularized emphysematous lungs did not survive beyond one week. However, cell attachment and proliferation on solubilized ECM homogenates of decellularized normal and emphysematous lungs coated onto tissue culture plates was comparable and not impaired, suggesting that the 3-dimensional decellularized emphysematous scaffolds may lack the necessary ECM architecture to support sustained cell growth. PMID:24461327

  14. Nuclear Ordered Phases of Solid 3He in Silver Sinters

    NASA Astrophysics Data System (ADS)

    Schuberth, Erwin A.; Kath, Matthias; Bago, Simone

    2006-09-01

    To determine the exact spin structure of the nuclear magnetic ordered phases of solid 3He, the U2D2 low field and the high field phases above 0.4 T, a European Research and Training Network for neutron scattering from the ordered solid was established which consisted of a collaboration with the Hahn Meitner Institute, Berlin, and other European and US groups. For this experiment it is crucial to grow a single crystal within the sinter needed for cooling the solid to temperatures of the order of 500 μK and to keep it cold long enough to measure a magnetic neutron diffraction. The sinter is also necessary to absorb the major part (> 90%) of the heat generated by the neutron capture and decay reaction of the 3He nucleus. In this work we studied the growth of crystals in Ag sinters of different pore sizes and with different growth speeds to find an optimal way to obtain single crystalline samples, or at least samples with only a few grains. We used SQUID magnetometry and NMR to measure the magnetization in the ordered phases. They were indicated by the known drop of the intensity, both in the NMR signal and in the dc magnetization, for the U2D2 phase, and by an increase of about 30% for the high field phase. The best results for cooling were obtained with sinters made from 700 Å "Japanese powder" with a packing fraction of 50% which were annealed at 130 °C after sintering and then had a calculated particle size of about 4200 Å. In the dc magnetization we found a paramagnetic surface contribution from a few monolayers of 3He down to 500 μK in addition to the bulk magnetization.

  15. Fermion Monte Carlo Calculations on Liquid-3He

    SciTech Connect

    Kalos, M H; Colletti, L; Pederiva, F

    2004-03-16

    Methods and results for calculations of the ground state energy of the bulk system of {sup 3}He atoms are discussed. Results are encouraging: they believe that they demonstrate that their methods offer a solution of the ''fermion sign problem'' and the possibility of direct computation of many-fermion systems with no uncontrolled approximations. Nevertheless, the method is still rather inefficient compared with variational or fixed-node approximate methods. There appears to be a significant populations size effect. The situation is improved by the inclusion of ''Second Stage Importance Sampling'' and of ''Acceptance/Rejection'' adapted to their needs.

  16. Hard Photodisintegration of Proton Pairs in {sup 3}He

    SciTech Connect

    Piasetzky, Eli; Pomerantz, Ishay; Higinbotham, D.; Strauch, S.; Gilman, R.

    2008-10-13

    Hard deuteron photodisintegration has been investigated for 20 years, as its cross section follows the constituent counting rule and it provides insight into the interplay between hadronic and quark-gluon degrees of freedom in high-momentum transfer exclusive reactions. We have now measured for the first time hard pp-pair disintegration in the reaction {gamma}{sup 3}He{yields}pp+n, using kinematics corresponding to a spectator neutron. Cross sections were measured for 90 deg. c.m. at 8 beam energies, from 0.8 to 4.7 GeV. Preliminary results will be presented and compared to the hard deuteron photodisintegration data.

  17. High-pressure /sup 3/He gas scintillation neutron spectrometer

    SciTech Connect

    Derzon, M.S.; Slaughter, D.R.; Prussin, S.G.

    1985-10-01

    A high-pressure, /sup 3/He-Xe gas scintillation spectrometer has been developed for neutron spectroscopy on D-D fusion plasmas. The spectrometer exhibits an energy resolution of (121 +- 20 keV) keV (FWHM) at 2.5 MeV and an efficiency of (1.9 +- 0.4) x 10/sup -3/ (n/cm/sup 2/)/sup -1/. The contribution to the resolution (FWHM) from counting statistics is only (22 +- 3 keV) and the remainder is due predominantly to the variation of light collection efficiency with location of neutron events within the active volume of the detector.

  18. High-momentum response of liquid 3He.

    PubMed

    Mazzanti, F; Polls, A; Boronat, J; Casulleras, J

    2004-02-27

    A final-state-effects formalism suitable to analyze the high-momentum response of Fermi liquids is presented and used to study the dynamic structure function of liquid 3He. The theory, developed as a natural extension of the Gersch-Rodriguez formalism, incorporates the Fermi statistics explicitly through a new additive term which depends on the semidiagonal two-body density matrix. The use of a realistic momentum distribution, calculated using the diffusion Monte Carlo method, and the inclusion of this additive correction allows for good agreement with available deep-inelastic neutron scattering data.

  19. Effective theory of superfluid 3He-A

    NASA Astrophysics Data System (ADS)

    Schakel, Adriaan M. J.; Batenburg, Peter

    1989-10-01

    From the symmetry content of the system an effective theory of superfluid 3He-A is derived. Two physically non-trivial implications of the theory are a relation between the chemical potential and the vorticity, and the observation that the superfluid cannot be accelerated if the vorticity is uniform. The theory also yields resolutions to two longstanding paradoxes, namely the mass current paradox and the angular momentum paradox. The resolution of the former turns out to provide a physical illustration of the Callan-Harvey effect, whereas the latter is resolved by utilizing Berry's geometrical phase.

  20. 3He Spin-Dependent Cross Sections and Sum Rules

    SciTech Connect

    Slifer, Karl; Amaryan, Moscov; Amaryan, Moskov; Auerbach, Leonard; Averett, Todd; Berthot, J.; Bertin, Pierre; Bertozzi, William; Black, Tim; Brash, Edward; Brown, D.; Burtin, Etienne; Calarco, John; Cates, Gordon; Chai, Zhengwei; Chen, Jian-Ping; Choi, Seonho; Chudakov, Eugene; Ciofi, Claudio; Cisbani, Evaristo; De Jager, Cornelis; Deur, Alexandre; DiSalvo, R.; Dieterich, Sonja; Djawotho, Pibero; Finn, John; Fissum, Kevin; Fonvieille, Helene; Frullani, Salvatore; Gao, Haiyan; Gao, Juncai; Garibaldi, Franco; Gasparian, Ashot; Gilad, Shalev; Gilman, Ronald; Glamazdin, Oleksandr; Glashausser, Charles; Glockle, W.; Golak, J.; Goldberg, Emma; Gomez, Javier; Gorbenko, Viktor; Hansen, Jens-Ole; Hersman, F.; Holmes, Richard; Huber, Garth; Hughes, Emlyn; Humensky, Thomas; Incerti, Sebastien; Iodice, Mauro; Jensen, S.; Jiang, Xiaodong; Jones, C.; Jones, G.; Jones, Mark; Jutier, Christophe; Kamada, H.; Ketikyan, Armen; Kominis, Ioannis; Korsch, Wolfgang; Kramer, Kevin; Kumar, Krishna; Kumbartzki, Gerfried; Kuss, Michael; Lakuriqi, Enkeleida; Laveissiere, Geraud; LeRose, John; Liang, Meihua; Liyanage, Nilanga; Lolos, George; Malov, Sergey; Marroncle, Jacques; McCormick, Kathy; McKeown, Robert; Meziani, Zein-Eddine; Michaels, Robert; Mitchell, Joseph; Nogga, Andreas; Pace, Emanuele; Papandreou, Zisis; Pavlin, Tina; Petratos, Gerassimos; Pripstein, David; Prout, David; Ransome, Ronald; Roblin, Yves; Rowntree, David; Rvachev, Marat; Sabatie, Franck; Saha, Arunava; Salme, Giovanni; SCOPETTA, S.; Skibinski, R.; Souder, Paul; Saito, Teijiro; Strauch, Steffen; Suleiman, Riad; Takahashi, Kazunori; Todor, Luminita; Tsubota, Hiroaki; Ueno, Hiroaki; Urciuoli, Guido; van der Meer, Rob; Vernin, Pascal; Voskanyan, Hakob; Witala, Henryk; Wojtsekhowski, Bogdan; Xiong, Feng; Xu, Wang; Yang, Jae-Choon; Zhang, Bin; Zolnierczuk, Piotr

    2008-07-01

    We present a measurement of the spin-dependent cross sections for the \\vec{^3He}(\\vec{e},e')X} reaction in the quasielastic and resonance regions at four-momentum transfer 0.1 < Q^2< 0.9 GeV^2. The spin-structure functions have been extracted and used to evaluate the nuclear Burkhardt--Cottingham and extended GDH sum rules for the first time. Impulse approximation and exact three-body Faddeev calculations are also compared to the data in the quasielastic region.

  1. Hard Photo-disintegration of proton pairs in 3He

    SciTech Connect

    Piasetzky, Eliazer; Pomerantz, Ishay; Higinbotham, Douglas; Strauch, Steffen; Gilman, Ronald

    2008-11-01

    Hard deuteron photodisintegration has been investigated for 20 years, as its cross section follows the constituent counting rule and it provides insight into the interplay between hadronic and quark-gluon degrees of freedom in high-momentum transfer exclusive reactions. We have now measured for the first time hard pp-pair disintegration in the reaction gamma 3He-->pp+n, using kinematics corresponding to a spectator neutron. Cross sections were measured for 90 deg. c.m. at 8 beam energies, from 0.8 to 4.7 GeV. Preliminary results will be presented and compared to the hard deuteron photodisintegration data.

  2. 3He spin-dependent cross sections and sum rules.

    PubMed

    Slifer, K; Amarian, M; Auerbach, L; Averett, T; Berthot, J; Bertin, P; Bertozzi, B; Black, T; Brash, E; Brown, D; Burtin, E; Calarco, J; Cates, G; Chai, Z; Chen, J-P; Choi, Seonho; Chudakov, E; Ciofi Degli Atti, C; Cisbani, E; de Jager, C W; Deur, A; DiSalvo, R; Dieterich, S; Djawotho, P; Finn, M; Fissum, K; Fonvieille, H; Frullani, S; Gao, H; Gao, J; Garibaldi, F; Gasparian, A; Gilad, S; Gilman, R; Glamazdin, A; Glashausser, C; Glöckle, W; Golak, J; Goldberg, E; Gomez, J; Gorbenko, V; Hansen, J-O; Hersman, B; Holmes, R; Huber, G M; Hughes, E; Humensky, B; Incerti, S; Iodice, M; Jensen, S; Jiang, X; Jones, C; Jones, G; Jones, M; Jutier, C; Kamada, H; Ketikyan, A; Kominis, I; Korsch, W; Kramer, K; Kumar, K; Kumbartzki, G; Kuss, M; Lakuriqi, E; Laveissiere, G; Lerose, J J; Liang, M; Liyanage, N; Lolos, G; Malov, S; Marroncle, J; McCormick, K; McKeown, R D; Meziani, Z-E; Michaels, R; Mitchell, J; Nogga, A; Pace, E; Papandreou, Z; Pavlin, T; Petratos, G G; Pripstein, D; Prout, D; Ransome, R; Roblin, Y; Rowntree, D; Rvachev, M; Sabatié, F; Saha, A; Salmè, G; Scopetta, S; Skibiński, R; Souder, P; Saito, T; Strauch, S; Suleiman, R; Takahashi, K; Teijiro, S; Todor, L; Tsubota, H; Ueno, H; Urciuoli, G; Van der Meer, R; Vernin, P; Voskanian, H; Witała, H; Wojtsekhowski, B; Xiong, F; Xu, W; Yang, J-C; Zhang, B; Zolnierczuk, P

    2008-07-11

    We present a measurement of the spin-dependent cross sections for the 3He over -->(e over -->,e')X reaction in the quasielastic and resonance regions at a four-momentum transfer 0.1< or =Q2< or =0.9 GeV2. The spin-structure functions have been extracted and used to evaluate the nuclear Burkhardt-Cottingham and extended Gerasimov-Drell-Hearn sum rules for the first time. The data are also compared to an impulse approximation calculation and an exact three-body Faddeev calculation in the quasielastic region.

  3. Inhaled histamine increases human lung mucociliary transport

    SciTech Connect

    Mussatto, D.J.; Garrard, C.S.; Trumbull, J.J.; Bowers, M.W.; Sanders, C.J.; Yeates, D.B.; Lourenco, R.V.

    1986-03-01

    Histamine, a mediator of airways constriction, alters ciliary beat frequency, bronchial mucus production, and epithelial ion transport; and in dogs, increases mucociliary transport. To evaluate the effect of inhaled histamine on human tracheobronchial mucociliary clearance, the authors measured lung mucociliary clearance (LMC) and tracheal mucociliary transport rate (TMTR) in 5 healthy, nonsmoking subjects in a randomized, double-blind, cross-over study. The concentration of inhaled histamine which produced a 20% fall in FEV/sub 1/ was established for each subject. On a separate day the subjects inhaled a 9 ..mu..m MMAD /sup 99m/Tc-Fe/sub 2/O/sub 3/ aerosol. LMC and TMTR were then measured for 2.5h using a gamma camera and a tracheal multidetector probe. Simultaneously, the subjects were challenged every 26 +/- 4 min with either PBS or histamine in PBS. The Fe/sub 2/O/sub 3/ retained after 24h for histamine (14.4 +/- 7.6%) and PBS studies (13.1 +/- 8.6%) indicated no difference in deposition of Fe/sub 2/O/sub 3/ (ANOVA). Fe/sub 2/O/sub 3/ clearance at 30 min was increased in the histamine studies (61 +/- 21% compared to the PBS studies (44 +/- 29%; p < 0.02, ANOVA)). TMTR was also increased with histamine (7.6 +/- 3.4 mm/min) compared to PBS (4.6 +/- 1.7 mm/min; p < 0.001, ANOVA). Results indicate an acute stimulatory effect of inhaled histamine on mucous transport in humans.

  4. Arsenic is Cytotoxic and Genotoxic to Primary Human Lung Cells

    PubMed Central

    Xie, Hong; Huang, ShouPing; Martin, Sarah; Wise, John P.

    2014-01-01

    Arsenic originates from both geochemical and numerous anthropogenic activities. Exposure of the general public to significant levels of arsenic is widespread. Arsenic is a well-documented human carcinogen. Long-term exposure to high levels of arsenic in drinking water have been linked to bladder, lung, kidney, liver, prostate, and skin cancer. Among them, lung cancer is of great public concern. However, little is known about how arsenic causes lung cancer and few studies have considered effects in normal human lung cells. The purpose of this study was to determine the cytotoxicity and genotoxicity of arsenic in human primary bronchial fibroblast and epithelial cells. Our data show that arsenic induces a concentration-dependent decrease in cell survival after short (24 h) or long (120 h) exposures. Arsenic induces concentration-dependent but not time-dependent increases in chromosome damage in fibroblasts. No chromosome damage is induced after either 24 h or 120 h arsenic exposure in epithelial cells. Using neutral comet assay and gamma-H2A.X foci forming assay, we found that 24 h or 120 h exposure to arsenic induces increases in DNA double strand breaks in both cell lines. These data indicate that arsenic is cytotoxic and genotoxic to human lung primary cells but lung fibroblasts are more sensitive to arsenic than epithelial cells. Further research is needed to understand the specific mechanisms involved in arsenic-induced genotoxicity in human lung cells. PMID:24291234

  5. Combined measurement of pulmonary inert gas washout and regional ventilation heterogeneity by MR of a single dose of hyperpolarized 3He.

    PubMed

    Deppe, Martin H; Parra-Robles, Juan; Ajraoui, Salma; Wild, Jim M

    2011-04-01

    Washout of inert gases is a measure of pulmonary function well-known in lung physiology. This work presents a method combining inert gas washout and spatially resolved imaging using hyperpolarized (3) He, thus providing complementary information on lung function and physiology. The nuclear magnetic resonance signal of intrapulmonary hyperpolarized (3) He is used to track the total amount of gas present within the lungs during multiple-breath washout via tidal breathing. Before the washout phase, 3D ventilation images are acquired using (3) He magnetic resonance imaging from the same dose of inhaled gas. The measured washout signal is corrected for T(1) relaxation and radiofrequency depletion, converting it into a quantity proportional to the apparent amount of gas within the lungs. The use of a pneumotachograph for acquisition of breathing volumes during washout, together with lung volumes derived from the magnetic resonance imaging data, permits assessment of the washout curves against physiological model predictions for healthy lungs. The shape of the resulting washout curves obtained from healthy volunteers matches the predictions, demonstrating the utility of the technique for the quantitative assessment of lung function. The proposed method can be readily integrated with a standard breath-hold (3) He ventilation imaging sequence, thus providing additional information from a single dose of gas.

  6. Textural domain walls in superfluid 3He-B

    NASA Astrophysics Data System (ADS)

    Mizushima, Takeshi

    Owing to the richness of symmetry, the superfluid 3He serves as a rich repository of topological quantum phenomena. This includes the emergence of surface Majorana fermions and their quantum mass acquisition at the topological critical point. Furthermore, the marriage of the prototype topological superfluid with nanofabrication techniques brings about a rich variety of spontaneous symmetry breaking, such as the formation of the stripe order and nontrivial domain walls. In this work, we examine the possible formation of textural domain walls in the superfluid 3He-B confined to a thin slab with a sub-micron thickness. When an applied magnetic field is much higher than the dipolar field, two nearly degenerate ground states appear, which are characterized by the Ising order associated with the spontaneous breaking of a magnetic order-two symmetry, lcirc;z = + 1 and - 1 . We here discuss the structure of the textural domain wall formed by the spatial modulation of the Ising order, such as low-lying quasiparticle excitations and spontaneous spin current. We also report bosonic modes bound to the textural domain wall.

  7. Duoplasmatron source modifications for {sup 3}He{sup +} Operation

    SciTech Connect

    Schmidt, C.W.; Popovic, M.

    1997-11-01

    A duoplasmatron ion source is used to produce 25 mA of {sup 3}He+ with a pulse width of {approximately}80 ms at 360 Hz for acceleration to 10.5 MeV. At this energy, {sup 3}He striking water or carbon targets can produce short lived isotopes of {sup 11}C, {sup 13}N, {sup 15}O and {sup 18}F for medical positron emission tomography (PET). A duoplasmatron ion source was chosen originally since it is capable of a sufficient singly-charged helium beam with an acceptable gas consumption. Stable long-term operation of the source required a change in the filament material to molybdenum, and a careful understanding of the oxide filament conditioning, operation and geometry. Other improvements, particularly in the electronics, were helpful to increasing the reliability. The source has operated for many months at {approximately}2.5% duty factor without significant problems and with good stability. We report here the effort that was done to make this source understandable and reliable.

  8. The 3H-3He Charge Radii Difference

    SciTech Connect

    Myers, Luke S.; Arrington, John R.; Higinbotham, Douglas W.

    2016-03-01

    The upcoming E12-14-009 [1] experiment at Jefferson Lab will determine the ratio of the electric form factors for the A=3 mirror nuclei 3He and 3H. The measurement will use a 1.1 GeV electron beam, a special collimator plate to allow for simultaneous optics measurements, and the low-activity tritium target being prepared for Jefferson Lab. By observing the dependence of the form factor ratio as a function of Q2 over 0.05–0.09 GeV2, the dependence of the radii extraction on the shape of the form factors is minimized. As a result, we anticipate the uncertainty of the extracted charge radii difference to be 0.03 fm, a reduction of 70% from the current measurement. Using precise measurements of the 3He charge radius from isotopic shift or μHe measurements [2–4], we can deduce the absolute 3H charge radius. The results will provide a direct comparison to recent calculations of the charge radii.

  9. Hard Photodisintegration of a Proton Pair in 3He

    SciTech Connect

    Brodsky, S.

    2003-09-30

    Hard photodisintegration of the deuteron has been extensively studied in order to understand the dynamics of the transition from hadronic to quark-gluon descriptions of the strong interaction. In this work, we discuss the extension of this program to hard photodisintegration of a pp pair in the {sup 3}He nucleus. Experimental confirmation of new features predicted here for the suggested reaction would advance our understanding of hard nuclear reactions. A main prediction, in contrast with low-energy observations, is that the pp breakup cross section is not much smaller than the one for pn break up.In some models, the energy-dependent oscillations observed for pp scattering are predicted to appear in the {gamma}{sup 3}He to pp + n reaction. Such an observation would open up a completely new field in studies of color coherence phenomena in hard nuclear reactions. We also demonstrate that, in addition to the energy dependence, the measurement of the light-cone momentum distribution of the recoil neutron provides an independent test of the underlying dynamics of hard disintegration.

  10. 3He film flow on a round rim beaker

    NASA Astrophysics Data System (ADS)

    Steel, S. C.; Harrison, J. P.; Zawadzki, P.; Sachrajda, A.

    1994-06-01

    The superfluid properties of thin (100 150 nm) of3He were investigated by measuring the rate at which a beaker of liquid3He emptied itself through the adsorbed film, with the film thickness δ decreasing as the level dropped. A beaker rim with a semicircular cross-section was used to provide a well defined geometry and to avoid the effects of small scratches that may have affected earlier experiments. The film thicknesses were determined by Atkins' oscillaton measurements of4He films on the same surface. The superfluid transition temperature in the film T {/c F } was suppressed below the bulk value T {/c B }, and was close to being described by 2δ/ξ( T {/c F }) = π, as expected for A-phase. The critical current density was more than an order of magnitude smaller than expected for pair-breaking. When a4He monolayer was adsorbed on the substrate, there was no suppresson of T {/c F }.

  11. Effective theory of 3H and 3He

    NASA Astrophysics Data System (ADS)

    König, Sebastian; Grießhammer, Harald W.; Hammer, H.-W.; van Kolck, U.

    2016-06-01

    We present a new perturbative expansion for pionless effective field theory with Coulomb interactions in which at leading order (LO) the spin-singlet nucleon-nucleon channels are taken in the unitarity limit. Presenting results up to next-to-leading order for the Phillips line and the neutron-deuteron doublet-channel phase shift, we find that a perturbative expansion in the inverse {}1{S}0 scattering lengths converges rapidly. Using a new systematic treatment of the proton-proton sector that isolates the divergence due to one-photon exchange, we renormalize the corresponding contribution to the {}3{{H}} -{}3{He} binding energy splitting and demonstrate that the Coulomb force in pionless EFT is a completely perturbative effect in the trinucleon bound-state regime. In our new expansion, the LO is exactly isospin-symmetric. At next-to-leading order, we include isospin breaking via the Coulomb force and two-body scattering lengths, and find for the energy splitting {({E}B{(}3{He})-{E}B{(}3{{H}}))}{NLO}\\quad =(-0.86+/- 0.17)\\quad {MeV}.

  12. Effect of Aerogel Anisotropy in Superfluid 3He-A

    NASA Astrophysics Data System (ADS)

    Zimmerman, A. M.; Li, J. I. A.; Pollanen, J.; Collett, C. A.; Gannon, W. J.; Halperin, W. P.

    2014-03-01

    Two theories have been advanced to describe the effects of anisotropic impurity introduced by stretched silica aerogel on the orientation of the orbital angular momentum l& circ; in superfluid 3He-A. These theories disagree on whether the anisotropy will orient l& circ; perpendicular[2] or parallel[3] to the strain axis. In order to examine this question we have produced and characterized a homogeneous aerogel sample with uniaxial anisotropy introduced during growth, corresponding to stretching of the aerogel. These samples have been shown to stabilize two new chiral states;[4] the higher temperature state being the subject of the present study. Using pulsed NMR we have performed experiments on 3He-A imbibed in this sample in two orientations: strain parallel and perpendicular to the applied magnetic field. From the NMR frequency shifts as a function of tip angle and temperature, we find that the angular momentum l& circ; is oriented along the strain axis, providing evidence for the theory advanced by Sauls. This work was supported by the National Science Foundation, DMR-1103625.

  13. Pion absorption on 3He at low energies

    NASA Astrophysics Data System (ADS)

    Hahn, H.; Altman, A.; Ashery, D.; Gefen, G.; Gill, D. R.; Johnson, R. R.; Levy-Nathansohn, R.; Moinester, M. A.; Sevior, M.; Trelle, R. P.

    1996-03-01

    The reactions 3He(π+,pp)p and 3He(π-,pn)n were studied at 37.0 MeV by coincidence detection of two nucleons. The differential cross sections were separated to two-nucleon (σ2N), three-nucleon (σ3N), and final-state interaction (σFSI) components. For π+, the σ2N angular distribution is symmetric about 90°, and the total cross section is 1.5 times the cross section measured for d(π+,pp). For π-, the angular distribution is asymmetric (backward peaked). The asymmetry increases with decreasing energy, indicating increasing pion s-wave contribution at lower energies. The fraction of the cross section induced by s-wave pions as calculated by a partial wave amplitude analysis is 13%. The measured total cross sections are σ2N(π-)=0.85+/-0.08 mb and σ2N(π+)=7.9+/-0.5 mb; σ3N(π-)=1.6+/-0.7 mb and σ3N(π+)=1.3+/-0.3 mb. A new evaluation of σ3N at Tπ=62.5 and 82.8 MeV is given, using data from an earlier experiment. The cross sections leading to the two-nucleon final-state interaction at Tπ=37.0 MeV are also estimated.

  14. 3He melting curve below 15 mK

    NASA Astrophysics Data System (ADS)

    Fukuyama, Hiroshi; Ishimoto, Hidehiko; Tazaki, Tetsurou; Ogawa, Shinji

    1987-12-01

    We have performed new measurements of the P-T relation along the 3He melting curve for temperatures between 0.4 and 15 mK in zero magnetic field. The temperature was determined by a Pt-wire nuclear-magnetic-resonance thermometer calibrated against the National Bureau of Standards scale (1983) above 15 mK. Three distinct points on the melting curve (the two superfluid transitions and the nuclear-spin ordering in the solid phase) were observed at temperatures lower than currently accepted values by about 10%. Our results are in good agreement with the P-T relation recently proposed by Greywall using a La-cerium magnesium nitrate thermometer, but differ seriously from the thermodynamic measurements by Halperin et al. From the measured melting curve, we could determine the ground-state energy of a nuclear spin in solid 3He to be -1.24 mK at the melting density. This value can be quantitatively explained by the current four-spin exchange theory.

  15. Interaction of Hydrogen Atoms with Helium Films: Sticking Probabilities for H on 3He and 4He, and the Binding Energy of H on 3He

    NASA Astrophysics Data System (ADS)

    Jochemsen, R.; Morrow, M.; Berlinsky, A. J.; Hardy, W. N.

    1981-09-01

    Magnetic resonance at 1420 MHz in zero magnetic field and for 0.063He, the rate constant for recombination and the frequency shift for H on 3He, and the sticking probability for H on 3He and 4He. The binding energy for H on liquid 3He is found to be 0.42+/-0.05 K, and the sticking probabilities are 0.035 for H on 4He and 0.016 for H on 3He.

  16. The accumulation of nickel in human lungs

    SciTech Connect

    Edelman, D.A.; Roggli, V.L. )

    1989-05-01

    Using data from published studies, lung concentrations of nickel were compare for persons with and without occupational exposure to nickel. As expected, the concentrations were much higher for persons with occupational exposure. To estimate the effects of nickel-containing tobacco smoke and nickel in the ambient air on the amount of nickel accumulated in lungs over time, a model was derived that took into account various variables related to the deposition of nickel in lungs. The model predicted nickel concentrations that were in the range of those of persons without known nickel exposure. Nickel is a suspected carcinogen and has been associated with an increased risk of respiratory tract cancer among nickel workers. However, before the nickel content of cigarettes can be implicated in the etiology of lung cancer, further studies are needed to evaluate the independent effects of smoking and exposure to nickel.

  17. The accumulation of nickel in human lungs.

    PubMed Central

    Edelman, D A; Roggli, V L

    1989-01-01

    Using data from published studies, lung concentrations of nickel were compare for persons with and without occupational exposure to nickel. As expected, the concentrations were much higher for persons with occupational exposure. To estimate the effects of nickel-containing tobacco smoke and nickel in the ambient air on the amount of nickel accumulated in lungs over time, a model was derived that took into account various variables related to the deposition of nickel in lungs. The model predicted nickel concentrations that were in the range of those of persons without known nickel exposure. Nickel is a suspected carcinogen and has been associated with an increased risk of respiratory tract cancer among nickel workers. However, before the nickel content of cigarettes can be implicated in the etiology of lung cancer, further studies are needed to evaluate the independent effects of smoking and exposure to nickel. PMID:2759060

  18. Flow Cytometric Analysis of Mononuclear Phagocytes in Nondiseased Human Lung and Lung-Draining Lymph Nodes

    PubMed Central

    Desch, A. Nicole; Gibbings, Sophie L.; Goyal, Rajni; Kolde, Raivo; Bednarek, Joe; Bruno, Tullia; Slansky, Jill E.; Jacobelli, Jordan; Mason, Robert; Ito, Yoko; Messier, Elise; Randolph, Gwendalyn J.; Prabagar, Miglena; Atif, Shaikh M.; Segura, Elodie; Xavier, Ramnik J.; Bratton, Donna L.; Janssen, William J.; Henson, Peter M.

    2016-01-01

    Rationale: The pulmonary mononuclear phagocyte system is a critical host defense mechanism composed of macrophages, monocytes, monocyte-derived cells, and dendritic cells. However, our current characterization of these cells is limited because it is derived largely from animal studies and analysis of human mononuclear phagocytes from blood and small tissue resections around tumors. Objectives: Phenotypic and morphologic characterization of mononuclear phagocytes that potentially access inhaled antigens in human lungs. Methods: We acquired and analyzed pulmonary mononuclear phagocytes from fully intact nondiseased human lungs (including the major blood vessels and draining lymph nodes) obtained en bloc from 72 individual donors. Differential labeling of hematopoietic cells via intrabronchial and intravenous administration of antibodies within the same lobe was used to identify extravascular tissue-resident mononuclear phagocytes and exclude cells within the vascular lumen. Multiparameter flow cytometry was used to identify mononuclear phagocyte populations among cells labeled by each route of antibody delivery. Measurements and Main Results: We performed a phenotypic analysis of pulmonary mononuclear phagocytes isolated from whole nondiseased human lungs and lung-draining lymph nodes. Five pulmonary mononuclear phagocytes were observed, including macrophages, monocyte-derived cells, and dendritic cells that were phenotypically distinct from cell populations found in blood. Conclusions: Different mononuclear phagocytes, particularly dendritic cells, were labeled by intravascular and intrabronchial antibody delivery, countering the notion that tissue and blood mononuclear phagocytes are equivalent systems. Phenotypic descriptions of the mononuclear phagocytes in nondiseased lungs provide a precedent for comparative studies in diseased lungs and potential targets for therapeutics. PMID:26551758

  19. Linear dimensions and volumes of human lungs

    SciTech Connect

    Hickman, David P.

    2012-03-30

    TOTAL LUNG Capacity is defined as “the inspiratory capacity plus the functional residual capacity; the volume of air contained in the lungs at the end of a maximal inspiration; also equals vital capacity plus residual volume” (from MediLexicon.com). Within the Results and Discussion section of their April 2012 Health Physics paper, Kramer et al. briefly noted that the lungs of their experimental subjects were “not fully inflated.” By definition and failure to obtain maximal inspiration, Kramer et. al. did not measure Total Lung Capacity (TLC). The TLC equation generated from this work will tend to underestimate TLC and does not improve or update total lung capacity data provided by ICRP and others. Likewise, the five linear measurements performed by Kramer et. al. are only representative of the conditions of the measurement (i.e., not at-rest volume, but not fully inflated either). While there was significant work performed and the data are interesting, the data does not represent a maximal situation, a minimal situation, or an at-rest situation. Moreover, while interesting, the linear data generated by this study is limited by the conditions of the experiment and may not be fully comparative with other lung or inspiratory parameters, measures, or physical dimensions.

  20. Linear dimensions and volumes of human lungs

    DOE PAGES

    Hickman, David P.

    2012-03-30

    TOTAL LUNG Capacity is defined as “the inspiratory capacity plus the functional residual capacity; the volume of air contained in the lungs at the end of a maximal inspiration; also equals vital capacity plus residual volume” (from MediLexicon.com). Within the Results and Discussion section of their April 2012 Health Physics paper, Kramer et al. briefly noted that the lungs of their experimental subjects were “not fully inflated.” By definition and failure to obtain maximal inspiration, Kramer et. al. did not measure Total Lung Capacity (TLC). The TLC equation generated from this work will tend to underestimate TLC and does notmore » improve or update total lung capacity data provided by ICRP and others. Likewise, the five linear measurements performed by Kramer et. al. are only representative of the conditions of the measurement (i.e., not at-rest volume, but not fully inflated either). While there was significant work performed and the data are interesting, the data does not represent a maximal situation, a minimal situation, or an at-rest situation. Moreover, while interesting, the linear data generated by this study is limited by the conditions of the experiment and may not be fully comparative with other lung or inspiratory parameters, measures, or physical dimensions.« less

  1. 3He specific heat and thermometry at millikelvin temperatures

    NASA Astrophysics Data System (ADS)

    Greywall, Dennis S.

    1986-06-01

    High-precision specific-heat measurements were made on pure liquid 3He in both the normal and superfluid phases for temperatures between 0.6 and 5 mK and for pressures between 0 and 34 bars. The data were obtained using a magnetic susceptibility thermometer which was calibrated against the National Bureau of Standards scale near 15 mK and at lower temperatures principally by the condition that the zero-pressure normal-phase specific heat be linear in temperature. The 3He phase diagram based on this scale is presented. In particular we find that TA=2.49 mK which differs quite substantially from the currently accepted value of about 2.7 mK. Multiplying the Pt NMR temperatures determined by Haavasoja and co-workers by a factor of 0.89 or subtracting 0.13 mK from the magnetic temperatures of Paulson et al. brings both of these scales into excellent agreement with the new scale. The 3He quasiparticle effective mass, m*3(P), extracted from the normal-phase data agrees well with our previously reported results based on higher-temperature specific-heat data. The values of m*3(P) from Haavasoja and co-workers are ~20% smaller. However, if their specific-heat data are reanalyzed using the new temperature scale, the two sets of m*3(P) values are brought into good agreement. We thus claim that the large discrepancies between previous specific-heat measurements are due almost entirely to differences in temperature scales. The new normal-phase specific-heat data at low pressures show no evidence of the anomalous behavior observed by Haavasoja and co-workers. Consequently, the size of the specific-heat jump at Tc could be determined with little ambiguity over the entire pressure range. ΔC/C> is only a few percent larger than the weak-coupling value at P=0 and increases linearly with sample density. At high density the temperature dependence of the specific heat below Tc shows small deviations from theory.

  2. Chiral phases of superfluid 3He in an anisotropic medium

    NASA Astrophysics Data System (ADS)

    Sauls, J. A.

    2013-12-01

    Recent advances in the fabrication and characterization of anisotropic silica aerogels with exceptional homogeneity provide new insight into the nature of unconventional pairing in disordered anisotropic media. I report theoretical analysis and predictions for the equilibrium phases of superfluid 3He infused into a low-density, homogeneous uniaxial aerogel. Ginzburg-Landau (GL) theory for a class of equal-spin-pairing (ESP) states in a medium with uniaxial anisotropy is developed and used to analyze recent experiments on uniaxially strained aerogels. For 3He in an axially “stretched” aerogel, GL theory predicts a transition from normal liquid into a chiral Anderson-Morel phase at Tc1 in which the chirality axis l̂ is aligned along the strain axis. This orbitally aligned state is protected from random fluctuations in the anisotropy direction, has a positive nuclear magnetic resonance (NMR) frequency shift, a sharp NMR resonance line, and is identified with the high-temperature ESP-1 phase of superfluid 3He in axially stretched aerogel. A second transition into a biaxial phase is predicted to onset at a slightly lower temperature Tc2

  3. Solubility of Freon 22 in human blood and lung tissue

    SciTech Connect

    Varene, N.; Choukroun, M.L.; Marthan, R.; Varene, P.

    1989-05-01

    The solubility of Freon 22 in human blood and lung tissue was determined using the chromatographic method of Wagner et al. In normal human blood, the mean Bunsen coefficient of solubility (alpha B) was 0.804 cm3 STPD.cm-3.ATA-1 at 37 degrees C. It increased with hematocrit (Hct) according to the equation alpha B = 0.274 Hct + 0.691. Tissue homogenates were prepared from macroscopically normal lung pieces obtained at thoracotomy from eight patients undergoing resection for lung carcinoma. The Bunsen solubility coefficients were 0.537 +/- 0.068 and 0.635 +/- 0.091 in washed and unwashed lung, respectively. These values can be used in the determination of both cardiac output and pulmonary tissue volume in humans by use of the rebreathing technique.

  4. Micro-imaging of the Mouse Lung via MRI

    NASA Astrophysics Data System (ADS)

    Wang, Wei

    Quantitative measurement of lung microstructure is of great significance in assessment of pulmonary disease, particularly in the earliest stages. Conventional stereological assessment of ex-vivo fixed tissue specimens under the microscope has a long and successful tradition and is regarded as a gold standard, but the invasive nature limits its applications and the practicality of use in longitudinal studies. The technique for diffusion MRI-based 3He lung morphometry was previously developed and validated for human lungs, and was recently extended to ex-vivo mouse lungs. The technique yields accurate, quantitative information about the microstructure and geometry of acinar airways. In this dissertation, the 3He lung morphometry technique is for the first time successfully implemented for in-vivo studies of mice. It can generate spatially-resolved maps of parameters that reveal the microstructure of mouse lung. Results in healthy mice indicate excellent agreement between in-vivo morphometry via 3He MRI and microscopic morphometry after sacrifice. The implementation and validation of 3He morphometry in healthy mice open up new avenues for application of the technique as a precise, noninvasive, in-vivo biomarker of changes in lung microstructure, within various mouse models of lung disease. We have applied 3He morphometry to the Sendai mouse model of lung disease. Specifically, the Sendai-virus model of chronic obstructive lung disease has demonstrated an innate immune response in mouse airways that exhibits similarities to the chronic airway inflammation in human COPD and asthma, but the effect on distal lung parenchyma had not been investigated. We imaged the time course and regional distribution of mouse lung microstructural changes in vivo after Sendai virus (SeV) infection with 1H and 3He diffusion MRI. 1H MR images detected the SeV-induced pulmonary inflammation in vivo and 3He lung morphometry showed modest increase in alveolar duct radius distal to airway

  5. Perspectives of hyperpolarized noble gas MRI beyond 3He

    PubMed Central

    Lilburn, David M.L.; Pavlovskaya, Galina E.; Meersmann, Thomas

    2013-01-01

    Nuclear Magnetic Resonance (NMR) studies with hyperpolarized (hp) noble gases are at an exciting interface between physics, chemistry, materials science and biomedical sciences. This paper intends to provide a brief overview and outlook of magnetic resonance imaging (MRI) with hp noble gases other than hp 3He. A particular focus are the many intriguing experiments with 129Xe, some of which have already matured to useful MRI protocols, while others display high potential for future MRI applications. Quite naturally for MRI applications the major usage so far has been for biomedical research but perspectives for engineering and materials science studies are also provided. In addition, the prospects for surface sensitive contrast with hp 83Kr MRI is discussed. PMID:23290627

  6. Alpha Backgrounds in the SNO ^3He Proportional Counter Array

    NASA Astrophysics Data System (ADS)

    Stonehill, Laura

    2006-04-01

    The Sudbury Neutrino Observatory (SNO) has recently deployed an array of proportional counters known as Neutral Current Detectors (NCDs) to detect thermalized neutrons via the ^3He(n,p)^3H reaction. The primary physics background to the neutron-capture signal is alpha particle emission from uranium- and thorium-chain decays in the NCD walls. The expected capture rate of neutrons from the neutral-current neutrino reaction on deuterium is three per day and the intrinsic alpha background rate is approximately 250 alphas per day. Fewer than 10% of these alphas fall into the energy range where neutron-capture signals occur, and a substantial number of these can be eliminated by pulse-shape analysis. This talk will focus on measurements of the alpha backgrounds in the NCDs and the extent to which these alphas contaminate the neutron-capture signal region.

  7. {sup 3}He target for Hall C at CEBAF

    SciTech Connect

    Zeidman, B.; Zeuli, A.

    1995-08-01

    A major fraction of the physics program for Hall C involves scattering from cryogenic targets of the lightest nuclei, i.e. H, D, and {sup 3,4}He. Argonne is constructing the He target that will consist of a 4cm cylinder, operating at a pressure of 10 atmospheres and a temperature of {approximately}5.2 degrees Kelvin. CEBAF is currently constructing a cryo-target system for liquid H and D cells and the cooled, pressurized helium targets. The He target system includes cell loop, the He supply systems, and the additional equipment needed to ensure minimum loss of {sup 3}He in the event of target rupture. Some of the major components have been completed, while the balance of the system will be ready for installation this fiscal year.

  8. Electrodisintegration of 3He below and above deuteron breakup threshold

    SciTech Connect

    Marcucci, L. E.; Viviani, M.; Schiavilla, R.; Kievsky, A.; Rosati, S.

    2005-02-01

    Recent advances in the study of electrodisintegration of 3He are presented and discussed. The pair-correlated hyperspherical harmonics method is used to calculate the initial and final state wave functions, with a realistic Hamiltonian consisting of the Argonne v18 two-nucleon and Urbana IX three-nucleon interactions. The model for the nuclear current and charge operators retains one- and many-body contributions. Particular attention is made in the construction of the two-body current operators arising from the momentum-dependent part of the two-nucleon interaction. Three-body current operators are also included so that the full current operator is strictly conserved. The present model for the nuclear current operator is tested comparing theoretical predictions and experimental data of pd radiative capture cross section and spin observables.

  9. Perspectives of hyperpolarized noble gas MRI beyond 3He.

    PubMed

    Lilburn, David M L; Pavlovskaya, Galina E; Meersmann, Thomas

    2013-04-01

    Nuclear Magnetic Resonance (NMR) studies with hyperpolarized (hp) noble gases are at an exciting interface between physics, chemistry, materials science and biomedical sciences. This paper intends to provide a brief overview and outlook of magnetic resonance imaging (MRI) with hp noble gases other than hp (3)He. A particular focus are the many intriguing experiments with (129)Xe, some of which have already matured to useful MRI protocols, while others display high potential for future MRI applications. Quite naturally for MRI applications the major usage so far has been for biomedical research but perspectives for engineering and materials science studies are also provided. In addition, the prospects for surface sensitive contrast with hp (83)Kr MRI is discussed. Copyright © 2012 Elsevier Inc. All rights reserved.

  10. Neutral beam injection in a D 3He FRC reactor

    NASA Astrophysics Data System (ADS)

    Ferrari, Hugo; Farengo, Ricardo

    2007-06-01

    The use of neutral beam injection (NBI) to sustain a fraction of the plasma current in a field reversed configuration (FRC) reactor operating with the D-3He reaction is studied. A Monte Carlo code already used to study NBI in medium size FRCs is employed (Lifschitz A F, Farengo R and Arista N R 2002 Nucl. Fusion 42 863, Lifschitz A F, Farengo R and Arista N R 2002 Plasma Phys. Control. Fusion 44 1979, Lifschitz A F, Farengo R and Hoffman A L 2004 Nucl. Fusion 44 1015) and the plasma parameters are similar to those proposed in the ARTEMIS (Momota H, Ishida A, Kohzaki Y, Miley G, Ohi S, Ohnishi M, Sato K, Steinhauer L, Tomita Y and Tuszewki M 1992 Fusion Technol. 21 2307) conceptual reactor design. A simple analysis shows that the driven current cannot reach the values quoted in the ARTEMIS project and a procedure to search for plasma parameters that result in higher efficiencies is presented.

  11. Perspectives of hyperpolarized noble gas MRI beyond 3He

    NASA Astrophysics Data System (ADS)

    Lilburn, David M. L.; Pavlovskaya, Galina E.; Meersmann, Thomas

    2013-04-01

    Nuclear Magnetic Resonance (NMR) studies with hyperpolarized (hp) noble gases are at an exciting interface between physics, chemistry, materials science and biomedical sciences. This paper intends to provide a brief overview and outlook of magnetic resonance imaging (MRI) with hp noble gases other than hp 3He. A particular focus are the many intriguing experiments with 129Xe, some of which have already matured to useful MRI protocols, while others display high potential for future MRI applications. Quite naturally for MRI applications the major usage so far has been for biomedical research but perspectives for engineering and materials science studies are also provided. In addition, the prospects for surface sensitive contrast with hp 83Kr MRI is discussed.

  12. Direct energy conversion system for D(3)-He fusion

    NASA Astrophysics Data System (ADS)

    Tomita, Y.; Shu, L. Y.; Momota, H.

    1993-11-01

    A novel and highly efficient direct energy conversion system is proposed for utilizing D(3)-He fueled fusion. In order to convert kinetic energy of ions, we applied a pair of direct energy conversion systems each of which has a cusp-type DEC and a traveling wave DEC (TWDEC). In a cusp-type DEC, electrons are separated from the escaping ions at the first line-cusp and the energy of thermal ion components is converted at the second cusp DEC. The fusion protons go through the cusp-type DEC and arrive at the TWDEC, which principle is similar to 'LINAC'. The energy of fusion protons is recovered to electricity with an efficiency of more than 70%. These DEC's bring about the high efficient fusion plant.

  13. 4He Versus 3He Josephson Effect: Vibration Decoherence

    SciTech Connect

    Pereverzev, Sergey V.

    2006-09-07

    Several on-going experiments searching for the Josephson effect in 4He close to the {lambda}-transition employ experimental cells with a weak link in the form of an array of submicron holes, with the size of the array and the cell dimensions very close to those used for 3He-B. In the same environment, the 4He experiment is more prone to decoherence by mechanical vibrations. The problem is due to the shift of the maximum of the vibration response of the experiment to low frequencies (0.1 Hz or less) and to the increase of the power spectrum density of the seismic velocities with decreasing frequency in this frequency range. To avoid decoherence, one needs to lower the cut-off frequency of the vibration isolation or to use an array with a larger open area. The latter option is briefly discussed.

  14. APT {sup 3}He target/blanket. Topical report

    SciTech Connect

    1995-03-01

    The {sup 3}He target/blanket (T/B) preconceptual design for the 3/8-Goal facility is based on a 1000-MeV, 200-mA accelerator to produce a high-intensity proton beam that is expanded and then strikes one of two T/B modules. Each module consists of a centralized neutron source made of tungsten and lead, a proton beam backstop region made of zirconium and lead, and a moderator made of D{sub 2}O. Helium-3 gas is circulated through the neutron source region and the blanket to create tritium through neutron capture. The gas is continually processed to extract the tritium with an online separation process.

  15. Human lung expresses unique gamma-glutamyl transpeptidase transcripts.

    PubMed Central

    Wetmore, L A; Gerard, C; Drazen, J M

    1993-01-01

    gamma-Glutamyl transpeptidase (EC 2.3.2.2, gamma GT) is a membrane-bound ectoenzyme that plays an important role in the metabolism of glutathione. It is composed of two subunits, both of which are encoded by a common mRNA. We examined the expression of gamma GT in human lung tissue by Northern blot analysis and screening a cDNA library made from human lung poly(A)+ RNA. Our results show that there are two gamma GT mRNA populations in human lung tissue. We define these as group I (2.4 kb) and group II (approximately 1.2 kb) transcripts. In the present communication, we characterize the unique lung transcript. Sequence analysis of representative clones shows that group I transcripts are virtually identical to those previously isolated from liver and placenta but possess a unique 5' untranslated region. In marked contrast, group II transcripts appear to be human-lung-specific. Group II transcripts appear on Northern blots probed with full-length or 3'-biased gamma GT cDNA. Sequence analysis of group II clones shows them to be homologous with group I clones in the region that encodes the reading frame for the light chain; however, they possess a series of unique 5' untranslated regions, which suggests that they arise from lung-specific message processing. Additionally, approximately 50% of the isolated group II clones contain 34 nt substitutions compared with the "wild-type" gamma GT transcripts. These data indicate that human lung expresses unique gamma GT transcripts of unknown function as well as the classical form. The abundant group II transcripts may encode part of a heterodimer related to gamma GT or represent processed lung-specific pseudogenes. Images Fig. 1 PMID:7689219

  16. Immune and Inflammatory Cell Composition of Human Lung Cancer Stroma

    PubMed Central

    Banat, G-Andre; Tretyn, Aleksandra; Pullamsetti, Soni Savai; Wilhelm, Jochen; Weigert, Andreas; Olesch, Catherine; Ebel, Katharina; Stiewe, Thorsten; Grimminger, Friedrich; Seeger, Werner; Fink, Ludger; Savai, Rajkumar

    2015-01-01

    Recent studies indicate that the abnormal microenvironment of tumors may play a critical role in carcinogenesis, including lung cancer. We comprehensively assessed the number of stromal cells, especially immune/inflammatory cells, in lung cancer and evaluated their infiltration in cancers of different stages, types and metastatic characteristics potential. Immunohistochemical analysis of lung cancer tissue arrays containing normal and lung cancer sections was performed. This analysis was combined with cyto-/histomorphological assessment and quantification of cells to classify/subclassify tumors accurately and to perform a high throughput analysis of stromal cell composition in different types of lung cancer. In human lung cancer sections we observed a significant elevation/infiltration of total-T lymphocytes (CD3+), cytotoxic-T cells (CD8+), T-helper cells (CD4+), B cells (CD20+), macrophages (CD68+), mast cells (CD117+), mononuclear cells (CD11c+), plasma cells, activated-T cells (MUM1+), B cells, myeloid cells (PD1+) and neutrophilic granulocytes (myeloperoxidase+) compared with healthy donor specimens. We observed all of these immune cell markers in different types of lung cancers including squamous cell carcinoma, adenocarcinoma, adenosquamous cell carcinoma, small cell carcinoma, papillary adenocarcinoma, metastatic adenocarcinoma, and bronchioloalveolar carcinoma. The numbers of all tumor-associated immune cells (except MUM1+ cells) in stage III cancer specimens was significantly greater than those in stage I samples. We observed substantial stage-dependent immune cell infiltration in human lung tumors suggesting that the tumor microenvironment plays a critical role during lung carcinogenesis. Strategies for therapeutic interference with lung cancer microenvironment should consider the complexity of its immune cell composition. PMID:26413839

  17. Follistatin is a novel biomarker for lung adenocarcinoma in humans.

    PubMed

    Chen, Fangfang; Ren, Ping; Feng, Ye; Liu, Haiyan; Sun, Yang; Liu, Zhonghui; Ge, Jingyan; Cui, Xueling

    2014-01-01

    Follistatin (FST), a single chain glycoprotein, is originally isolated from follicular fluid of ovary. Previous studies have revealed that serum FST served as a biomarker for pregnancy and ovarian mucinous tumor. However, whether FST can serve as a biomarker for diagnosis in lung adenocarcinoma of humans remains unclear. The study population consisted of 80 patients with lung adenocarcinoma, 40 patients with ovarian adenocarcinoma and 80 healthy subjects. Serum FST levels in patients and healthy subjects were measured using ELISA. The results showed that the positive ratio of serum FST levels was 51.3% (41/80), which was comparable to the sensitivity of FST in 40 patients with ovarian adenocarcinoma (60%, 24/40) using the 95th confidence interval for the healthy subject group as the cut-off value. FST expressions in lung adenocarcinoma were examined by immunohistochemical staining, we found that lung adenocarcinoma could produce FST and there was positive correlation between the level of FST expression and the differential degree of lung adenocarcinoma. Furthermore, the results showed that primary cultured lung adenocarcinoma cells could secrete FST, while cells derived from non-tumor lung tissues almost did not produce FST. In addition, the results of CCK8 assay and flow cytometry showed that using anti-FST monoclonal antibody to neutralize endogenous FST significantly augmented activin A-induced lung adenocarcinoma cells apoptosis. These data indicate that lung adenocarcinoma cells can secret FST into serum, which may be beneficial to the survival of adenocarcinoma cells by neutralizing activin A action. Thus, FST can serve as a promising biomarker for diagnosis of lung adenocarcinoma and a useful biotherapy target for lung adenocarcinoma.

  18. Follistatin Is a Novel Biomarker for Lung Adenocarcinoma in Humans

    PubMed Central

    Feng, Ye; Liu, Haiyan; Sun, Yang; Liu, Zhonghui; Ge, Jingyan; Cui, Xueling

    2014-01-01

    Background Follistatin (FST), a single chain glycoprotein, is originally isolated from follicular fluid of ovary. Previous studies have revealed that serum FST served as a biomarker for pregnancy and ovarian mucinous tumor. However, whether FST can serve as a biomarker for diagnosis in lung adenocarcinoma of humans remains unclear. Methods and Results The study population consisted of 80 patients with lung adenocarcinoma, 40 patients with ovarian adenocarcinoma and 80 healthy subjects. Serum FST levels in patients and healthy subjects were measured using ELISA. The results showed that the positive ratio of serum FST levels was 51.3% (41/80), which was comparable to the sensitivity of FST in 40 patients with ovarian adenocarcinoma (60%, 24/40) using the 95th confidence interval for the healthy subject group as the cut-off value. FST expressions in lung adenocarcinoma were examined by immunohistochemical staining, we found that lung adenocarcinoma could produce FST and there was positive correlation between the level of FST expression and the differential degree of lung adenocarcinoma. Furthermore, the results showed that primary cultured lung adenocarcinoma cells could secrete FST, while cells derived from non-tumor lung tissues almost did not produce FST. In addition, the results of CCK8 assay and flow cytometry showed that using anti-FST monoclonal antibody to neutralize endogenous FST significantly augmented activin A-induced lung adenocarcinoma cells apoptosis. Conclusions These data indicate that lung adenocarcinoma cells can secret FST into serum, which may be beneficial to the survival of adenocarcinoma cells by neutralizing activin A action. Thus, FST can serve as a promising biomarker for diagnosis of lung adenocarcinoma and a useful biotherapy target for lung adenocarcinoma. PMID:25347573

  19. MR Imaging of Apparent 3He Gas Transport in Narrow Pipes and Rodent Airways

    SciTech Connect

    Minard, Kevin R.; Jacob, Rick E.; Laicher, Gernot; Einstein, Daniel R.; Kuprat, Andrew P.; Corley, Richard A.

    2008-10-01

    High sensitivity makes hyperpolarized 3He an attractive signal source for visualizing gas flow with magnetic resonance (MR) imaging. Its rapid Brownian motion, however, can blur observed flow lamina and alter measured diffusion rates when excited nuclei traverse shear-induced velocity gradients during data acquisition. Here, both effects are described analytically, and predicted values for measured transport during laminar flow through a straight, 3.2-mm-diameter pipe are validated using two-dimensional (2D) constant-time images of different binary gas mixtures. Results show explicitly how measured transport in narrow conduits is characterized by apparent values that depend on underlying gas dynamics and imaging time. In ventilated rats, this is found to obscure acquired airflow images. Flow splitting at airway branches is still evident, however, and use of 3D vector flow mapping is shown to provide a quantitative view of pulmonary gas supply that highlights the correlation of airflow dynamics with lung structure.

  20. Ex Vivo Perfusion Treatment of Infection in Human Donor Lungs.

    PubMed

    Nakajima, D; Cypel, M; Bonato, R; Machuca, T N; Iskender, I; Hashimoto, K; Linacre, V; Chen, M; Coutinho, R; Azad, S; Martinu, T; Waddell, T K; Hwang, D M; Husain, S; Liu, M; Keshavjee, S

    2016-04-01

    Ex vivo lung perfusion (EVLP) is a platform to treat infected donor lungs with antibiotic therapy before lung transplantation. Human donor lungs that were rejected for transplantation because of clinical concern regarding infection were randomly assigned to two groups. In the antibiotic group (n = 8), lungs underwent EVLP for 12 h with high-dose antibiotics (ciprofloxacin 400 mg or azithromycin 500 mg, vancomycin 15 mg/kg, and meropenem 2 g). In the control group (n = 7), lungs underwent EVLP for 12 h without antibiotics. A quantitative decrease in bacterial counts in bronchoalveolar lavage (BAL) was found in all antibiotic-treated cases but in only two control cases. Perfusate endotoxin levels at 12 h were significantly lower in the antibiotic group compared with the control group. EVLP with broad-spectrum antibiotic therapy significantly improved pulmonary oxygenation and compliance and reduced pulmonary vascular resistance. Perfusate endotoxin levels at 12 h were strongly correlated with levels of perfusates tumor necrosis factor α, IL-1β and macrophage inflammatory proteins 1α and 1β at 12 h. In conclusion, EVLP treatment of infected donor lungs with broad-spectrum antibiotics significantly reduced BAL bacterial counts and endotoxin levels and improved donor lung function. © Copyright 2015 The American Society of Transplantation and the American Society of Transplant Surgeons.

  1. Decreased expression of interleukin 13 in human lung emphysema

    PubMed Central

    Boutten, A; Bonay, M; Laribe, S; Leseche, G; Castier, Y; Lecon-Malas, V; Fournier, M; Durand, G; Aubier, M; Dehoux, M; Crestani, B

    2004-01-01

    Background: The overexpression of interferon (IFN)γ or interleukin (IL)-13 in the adult murine lung induces the development of changes that mirror human lung emphysema. Methods: IL-13 and IFNγ expression was determined in lung samples from five groups of patients: severe emphysema without α1-antitrypsin deficiency (SE+, n = 10); severe emphysema with α1-antitrypsin deficiency (SE–, n = 5); mild localised emphysema (ME, n = 8); non-emphysema smokers (NE-S, n = 9), and non-emphysema non-smokers (NE-NS, n = 11). Lung IL-13 and IFNγ mRNA were analysed by RT-PCR. Lung concentrations of IL-13 protein were assessed by ELISA. Results: The expression of IFNγ mRNA was similar in patients with or without emphysema. IL-13 mRNA was markedly decreased in the SE+ group compared with the SE– (p = 0.04), ME (p = 0.02), and non-emphysema groups (p = 0.01). IL-13 mRNA correlated with forced expiratory volume in 1 second (r = 0.5, p = 0.04) and arterial oxygen tension (r = 0.45, p = 0.03) in emphysema patients. In contrast to the non-emphysematous lung, IL-13 protein was below the detection limit of the assay in most emphysematous lung homogenates. Conclusion: The lung IL-13 content is reduced in patients with severe emphysema without α1-antitrypsin deficiency. PMID:15454650

  2. Neutron multiplicity ,easurements With 3He alternative: Straw neutron detectors

    DOE PAGES

    Mukhopadhyay, Sanjoy; Wolff, Ronald S.; Meade, John A.; ...

    2015-01-27

    Counting neutrons emitted by special nuclear material (SNM) and differentiating them from the background neutrons of various origins is the most effective passive means of detecting SNM. Unfortunately, neutron detection, counting, and partitioning in a maritime environment are complex due to the presence of high-multiplicity spallation neutrons (commonly known as “ship effect”) and to the complicated nature of the neutron scattering in that environment. In this study, a prototype neutron detector was built using 10B as the converter in a special form factor called “straws” that would address the above problems by looking into the details of multiplicity distributions ofmore » neutrons originating from a fissioning source. This paper describes the straw neutron multiplicity counter (NMC) and assesses the performance with those of a commercially available fission meter. The prototype straw neutron detector provides a large-area, efficient, lightweight, more granular (than fission meter) neutron-responsive detection surface (to facilitate imaging) to enhance the ease of application of fission meters. Presented here are the results of preliminary investigations, modeling, and engineering considerations leading to the construction of this prototype. This design is capable of multiplicity and Feynman variance measurements. This prototype may lead to a near-term solution to the crisis that has arisen from the global scarcity of 3He by offering a viable alternative to fission meters. This paper describes the work performed during a 2-year site-directed research and development (SDRD) project that incorporated straw detectors for neutron multiplicity counting. The NMC is a two-panel detector system. We used 10B (in the form of enriched boron carbide: 10B4C) for neutron detection instead of 3He. In the first year, the project worked with a panel of straw neutron detectors, investigated its characteristics, and developed a data acquisition (DAQ) system to collect

  3. Optical interferometry in superfluid {sup 3}He-B

    SciTech Connect

    Alles, H.; Ruutu, J.P.; Babkin, A.V.; Hakonen, P.J.; Sonin, E.B.

    1996-03-01

    The authors report interferometric measurements in 0.1...1 mm thick films of superfluid {sup 3}He-B. The menisci of three different rotational states of the superfluid were observed and analyzed theoretically using two-fluid hydrodynamics: These are (i) the equilibrium vortex state in which the superfluid and the normal components corotate (solid body rotation), (ii) the vortex-free state (the Landau state), in which only the normal component rotates, and (iii) the quasistationary vortex state in which only the superfluid fraction rotates (pure superfluid rotation). The Landua state manifested itself by a reduced parabolic meniscus at rotation speeds below the critical angular velocity {Omega}{sub c}{approx_lt} 0.2 rad/s for vortex formation. Transition from the Landua state to the equilibrium vortex state yielded a sudden deepening of the meniscus when {Omega}{sub c} was exceeded. After a rapid halt of the cryostat, the authors observed a novel meniscus which was produced by the superfluid rotation while the normal component was at rest. The enhanced depth of this meniscus is governed by the reactive mutual friction parameter B{prime}. By employing laser light, both for imaging and for thermomechanical excitation, the authors measured the response of a thin superfluid layer to a heat pulse and analyzed it within the theory of two fluid hydrodynamics. The data were employed, using the dispersion relation for thin film oscillations, to deduce the second viscosity coefficient {zeta}{sub 3} close to T{sub c}.

  4. Spin Pumping in Superfluid ^3He in High Magnetic Field

    NASA Astrophysics Data System (ADS)

    Kojima, H.; Suzuki, K.; Aoki, Y.; Yamaguchi, A.; Ishimoto, H.

    2008-03-01

    The spin flow dynamics in superfluid ^3He A1 phase in magnetic field has been studied up to 13 tesla. The apparatus consists of a large reservoir of of A1 phase in which a small enclosed chamber with a built-in differential pressure sensor is immersed. The chamber is connected to the reservoir via a superleak channel. The chamber is fabricated from Macor parts such that the residual heat leak is much reduced from those in our experiments. Our focus is on the measurement of relaxation of the induced pressure subsequent to either magnetically induced spin-polarized superflow or by electrostatic spin pumping. In general, both methods of measurement show that the relaxation time (τ) of the induced pressure tends to vanish smoothly as the transition temperature Tc2 is approached. However, the observed dependence of τ on magnetic field is different. The measured τ by the field gradient method continues to increase up to 8 tesla. On the other hand, τ measured by the spin pumping method tends to saturate to a constant between 5 and 13 tesla. The discrepancy is unexpected and not yet understood.

  5. Andreev reflection in rotating superfluid {sup 3}He-B

    SciTech Connect

    Eltsov, V. B.; Hosio, J. J.; Krusius, M. Mäkinen, J. T.

    2014-12-15

    Andreev reflection of quasiparticle excitations from quantized line vortices is reviewed in the isotropic B phase of superfluid {sup 3}He in the temperature regime of ballistic quasiparticle transport at T ≤ 0.20T{sub c}. The reflection from an array of rectilinear vortices in solid-body rotation is measured with a quasiparticle beam illuminating the array mainly in the orientation along the rotation axis. The result is in agreement with the calculated Andreev reflection. The Andreev signal is also used to analyze the spin-down of the superfluid component after a sudden impulsive stop of rotation from an equilibrium vortex state. In a measuring setup where the rotating cylinder has a rough bottom surface, annihilation of the vortices proceeds via a leading rapid turbulent burst followed by a trailing slow laminar decay, from which the mutual friction dissipation can be determined. In contrast to the currently accepted theory, it is found to have a finite value in the zero-temperature limit: α(T→0) = (5 ± 0.5) × 10{sup −4}.

  6. Hard photodisintegration of 3He into a p d pair

    NASA Astrophysics Data System (ADS)

    Maheswari, Dhiraj; Sargsian, Misak M.

    2017-02-01

    The recent measurements of high energy photodisintegration of a 3He nucleus to a p d pair at 90∘ center of mass demonstrated an energy scaling consistent with the quark counting rule with an unprecedentedly large exponent of s-17. To understand the underlying mechanism of this process, we extended the theoretical formalism of the hard rescattering mechanism (HRM) to calculate the γ 3He→p d reaction. In HRM the incoming high energy photon strikes a quark from one of the nucleons in the target which subsequently undergoes hard rescattering with the quarks from the other nucleons, generating a hard two-body system in the final state of the reaction. Within the HRM we derived the parameter-free expression for the differential cross section of the reaction, which is expressed through the 3He→p d transition spectral function, the cross section of hard p d →p d scattering, and the effective charge of the quarks being interchanged during the hard rescattering process. The numerical estimates of all these factors resulted in the magnitude of the cross section, which is surprisingly in good agreement with the data.

  7. Proton-{sup 3}He elastic scattering at low energies

    SciTech Connect

    Fisher, B. M.; Brune, C. R.; Karwowski, H. J.; Leonard, D. S.; Ludwig, E. J.; Black, T. C.; Viviani, M.; Kievsky, A.; Rosati, S.

    2006-09-15

    We present new accurate measurements of the differential cross section {sigma}({theta}) and the proton analyzing power A{sub y} for proton-{sup 3}He elastic scattering at various energies. A supersonic gas-jet target has been employed to obtain these low-energy cross-section measurements. The {sigma}({theta}) distributions have been measured at E{sub p}=0.99, 1.59, 2.24, 3.11, and 4.02 MeV. Full angular distributions of A{sub y} have been measured at E{sub p}=1.60, 2.25, 3.13, and 4.05 MeV. This set of high-precision data is compared to four-body variational calculations employing realistic nucleon-nucleon (NN) and three-nucleon (3N) interactions. For the unpolarized cross section, the agreement between the theoretical calculation and data is good when a 3N potential is used. The comparison between the calculated and measured proton analyzing powers reveals discrepancies of approximately 50% at the maximum of each distribution. This is analogous to the existing 'A{sub y} puzzle' known for the past 20 years in nucleon-deuteron elastic scattering.

  8. Non-Flux-Rope CME-Driven Shocks and Connection to Particle Acceleration in Solar 3He-Rich Events

    NASA Astrophysics Data System (ADS)

    Zhang, T.; Wu, S.

    2010-12-01

    Two most significant space weather drivers that cause a wide range of severe effects on human activities are solar energetic particles (SEPs) and coronal mass ejections (CMEs). Observations at 1 AU indicated that the most frequently observed CMEs (about 2/3 of events) do not have flux-rope structure and the most frequently observed SEPs (about 1000 events per year) enrich their 3He abundances. 3He is a most important and clean fuel of thermonuclear fusion. It is rather rare on Earth, but its abundance is extremely enhanced in the satellite material and the Moon’s upper layer of the regolith due to the 3He-enrichments in SEP events. Recently, the initiation of CMEs without flux-rope topology have been simulated with a three-dimensional, time-dependent, self-consistent, flux emergence and reconnection, magnetohydrodynamic (MHD) model. This was done through emerging magnetic flux of one polarity from the photosphere, beneath the open field lines of the opposite polarity near the closed magnetic field lines. The results indicated that the magnetic flux emergence and reconnection can produce the magnetic topology that lead to solar 3He-rich events and generate jet-like plasma outflows, which may be further developed into CMEs without magnetic flux-rope topology if enough energy is deposited. This study examines the properties of non-flux-rope CME driven shocks and the connection to the particle acceleration in solar 3He-rich events.

  9. Transfer Excitation Processes Observed in N3+-He and O3+-He Collisions at Elab = 33 eV

    NASA Astrophysics Data System (ADS)

    Itoh, Yoh

    2016-09-01

    We measured the relative state-selective differential cross sections (DCSs) for one-electron capture reactions using a crossed-beam apparatus. The scattering angle θlab studied in the laboratory frame ranged from -3.0 to 22° and the laboratory collision energy Elab was 33 eV. Only the transfer excitation processes, i.e., the electron capture reactions with the simultaneous excitation of the projectile, were observed. The DCSs were determined for the following reactions: N3+ (1s2 2s2 1S) + He (1s2 1S) → N2+ (1s2 2s2p2 2D) + He+ (1s 2S) + 10.3 eV, O3+ (1s2 2s2 2p 2P) + He (1s2 1S) → O2+ (1s2 2s 2p3 3P) + He+ (1s 2S) + 12.7 eV, and O3+ (1s2 2s2 2p 2P) + He (1s2 1S) → O2+ (1s2 2s 2p3 3D) + He+ (1s 2S) + 15.5 eV. In the N3+-He system, the DCSs for the reaction are zero at the center-of-mass angle θcm = 0 and show a peak at a certain angle and a shoulder at a larger angle. In the O3+-He system, the DCSs are again zero at θcm = 0. The capture process to the O2+ (1s2 2s 2p3 3P) state is mainly observed at smaller scattering angles, and the reaction to the O2+ (1s2 2s 2p3 3D) state becomes dominant with increasing scattering angle. A classical trajectory analysis within the two-state approximation based on the ab initio potentials for (NHe)3+ revealed that the transfer excitation of a two-electron process takes place through a single crossing of the relevant potentials.

  10. Second-hand smoke and human lung cancer

    PubMed Central

    Besaratinia, Ahmad; Pfeifer, Gerd P.

    2009-01-01

    Since the early 1980s, there has been growing concern about potential health consequences of exposure to second-hand smoke (SHS). Despite SHS being established as a risk factor for lung cancer development, the estimated risk has remained small yet somehow debatable. Human exposure to SHS is complicated because of temporal variabilities in source, composition, and concentration of SHS. The temporality of exposure to SHS is important for human lung carcinogenesis with a latency of many years. To explore the causal effect of SHS in lung carcinogenesis, exposure assessments should estimate chronic exposure to SHS on an individual basis. However, conventional exposure assessment for SHS relies on one-off or short-term measurements of SHS indices. A more reliable approach would be to use biological markers that are specific for SHS exposure and pertinent to lung cancer. This approach requires an understanding of the underlying mechanisms through which SHS could contribute to lung carcinogenesis. This Review is a synopsis of research on SHS and lung cancer, with special focus on hypothetical modes of action of SHS for carcinogenesis, including genotoxic and epigenetic effects. PMID:18598930

  11. Comment on "Deep mixing of 3He: reconciling Big Bang and stellar nucleosynthesis".

    PubMed

    Balser, Dana S; Rood, Robert T; Bania, T M

    2007-08-31

    Eggleton et al. (Reports, 8 December 2006, p. 1580) reported on a deep-mixing mechanism in low-mass stars caused by a Rayleigh-Taylor instability that destroys all of the helium isotope 3He produced during the star's lifetime. Observations of 3He in planetary nebulae, however, indicate that some stars produce prodigious amounts of 3He. This is inconsistent with the claim that all low-mass stars should destroy 3He.

  12. TMEM45B, up-regulated in human lung cancer, enhances tumorigenicity of lung cancer cells.

    PubMed

    Hu, Rui; Hu, Fengqing; Xie, Xiao; Wang, Lei; Li, Guoqing; Qiao, Tong; Wang, Mingsong; Xiao, Haibo

    2016-09-01

    Transmembrane protein 45B (TMEM45B) is a member of TMEMs. Altered expression of TMEMs is frequently observed in a variety of human cancers, but the expression and functional roles of TMEM45B in lung cancer is not reported. In the present study, levels of mRNA expression of TMEM45B in lung cancer tissues were assessed using re-analyzing expression data of The Cancer Genome Atlas (TCGA) lung cancer cohort and real-time PCR analysis on our own cohort. Lung cancer cells, A549 and NCI-H1975, infected with TMEM45B short hairpin RNA were examined in cell proliferation, cell cycle, cell apoptosis, wound-healing, and cell invasion assays as well as mouse xenograft models. Here, we demonstrated that TMEM45B was overexpressed in lung cancer and its expression correlated with overall survival of patients. In addition, silencing of TMEM45B expression reduced cell proliferation in vitro and in vivo, induced cell cycle arrest and cell apoptosis, and blocked cell migration and invasion. Moreover, knockdown of TMEM45B significantly suppressed G1/S transition, induced cell apoptosis, and inhibited cell invasion via regulating the expression of cell cycle-related proteins (CDK2, CDC25A, and PCNA), cell apoptosis-related proteins (Bcl2, Bax, and Cleaved Caspase 3), and metastasis-related proteins (MMP-9, Twist, and Snail), respectively. Thus, TMEM45B is a potential prognostic marker and cancer-selective therapeutic target in lung cancer.

  13. Acute lung injury after instillation of human breast milk or infant formula into rabbits' lungs.

    PubMed

    O'Hare, B; Lerman, J; Endo, J; Cutz, E

    1996-06-01

    Recent interest in shortening the fasting interval after ingestion of milk products demonstrated large volumes of breast milk in the stomach 2 h after breastfeeding. Although aspiration is a rare event, if it were to occur with human breast milk, it is important to understand the extent of the lung injury that might occur. Therefore, the response to instillation of acidified breast milk and infant formula in the lungs of adult rabbits was studied. In 18 anesthetized adult rabbits, 1 of 3 fluids (in a volume of 0.8 ml.kg-1 and pH level of 1.8, acidified with hydrochloric acid); saline, breast milk, or infant formula (SMA, Wyeth, Windsor, Ontario), was instilled into the lungs via a tracheotomy. The lungs were ventilated for 4 h after instillation. Alveolar-to-arterial oxygen gradient and dynamic compliance were measured before and at hourly intervals after instillation. After 4 h, the rabbits were killed and the lungs were excised. Neutrophil infiltration was quantitated by a pathologist blinded to the instilled fluid. A histologic control group of four rabbits was ventilated under study conditions without any intratracheal fluid instillation. Alveolar-to-arterial oxygen gradient increased and dynamic compliance decreased significantly during the 4 h after instillation of both breast milk and infant formula compared with baseline measurements and with saline controls (P < 0.05). The neutrophil counts in the lungs from the saline, breast milk, and formula rabbits were significantly greater than those in the control group. Instillation of acidified breast milk or infant formula (in a volume of 0.8 ml.kg-1 and pH level of 1.8) into rabbits' lungs induces acute lung injury of similar intensity that lasts at least 4 h.

  14. Velocity of second sound in /sup 3/He-/sup 4/He mixtures: a revision

    SciTech Connect

    Bowley, R.M.

    1988-05-01

    Greywall has recently proposed a new temperature scale for the /sup 3/He melting curve thermometer. A reanalysis of the velocity of second sound in /sup 3/He-/sup 4/He mixtures using the data of Greywall and Paalanen with this new temperature scale shows that the interaction between /sup 3/He quasiparticles is now independent of concentration.

  15. Temporal Variability in the Accretion Rate of Interplanetary Dust Using (3)He as a Tracer

    NASA Technical Reports Server (NTRS)

    Farley, K. A.

    2005-01-01

    The research supported by this grant falls under three topics: 1) Weekly Interplanetary Dust Sampling via (3)He; 2) Extraterrestrial (3)He at Major Impact Boundaries; 3) Completing a Moderately-High Resolution Record of Extraterrestrial (3)He Flux: A Major Asteroidal Break up Event at 8.2 Ma.

  16. Impact of Statins on Gene Expression in Human Lung Tissues

    PubMed Central

    Lane, Jérôme; van Eeden, Stephan F.; Obeidat, Ma’en; Sin, Don D.; Tebbutt, Scott J.; Timens, Wim; Postma, Dirkje S.; Laviolette, Michel; Paré, Peter D.; Bossé, Yohan

    2015-01-01

    Statins are 3-hydroxy-3-methylglutaryl-coenzyme A reductase inhibitors that alter the synthesis of cholesterol. Some studies have shown a significant association of statins with improved respiratory health outcomes of patients with asthma, chronic obstructive pulmonary disease and lung cancer. Here we hypothesize that statins impact gene expression in human lungs and may reveal the pleiotropic effects of statins that are taking place directly in lung tissues. Human lung tissues were obtained from patients who underwent lung resection or transplantation. Gene expression was measured on a custom Affymetrix array in a discovery cohort (n = 408) and two replication sets (n = 341 and 282). Gene expression was evaluated by linear regression between statin users and non-users, adjusting for age, gender, smoking status, and other covariables. The results of each cohort were combined in a meta-analysis and biological pathways were studied using Gene Set Enrichment Analysis. The discovery set included 141 statin users. The lung mRNA expression levels of eighteen and three genes were up-regulated and down-regulated in statin users (FDR < 0.05), respectively. Twelve of the up-regulated genes were replicated in the first replication set, but none in the second (p-value < 0.05). Combining the discovery and replication sets into a meta-analysis improved the significance of the 12 up-regulated genes, which includes genes encoding enzymes and membrane proteins involved in cholesterol biosynthesis. Canonical biological pathways altered by statins in the lung include cholesterol, steroid, and terpenoid backbone biosynthesis. No genes encoding inflammatory, proteases, pro-fibrotic or growth factors were altered by statins, suggesting that the direct effect of statin in the lung do not go beyond its antilipidemic action. Although more studies are needed with specific lung cell types and different classes and doses of statins, the improved health outcomes and survival observed in statin

  17. RECONSTRUCTION OF HUMAN LUNG MORPHOLOGY MODELS FROM MAGNETIC RESONANCE IMAGES

    EPA Science Inventory


    Reconstruction of Human Lung Morphology Models from Magnetic Resonance Images
    T. B. Martonen (Experimental Toxicology Division, U.S. EPA, Research Triangle Park, NC 27709) and K. K. Isaacs (School of Public Health, University of North Carolina, Chapel Hill, NC 27514)

  18. RECONSTRUCTION OF HUMAN LUNG MORPHOLOGY MODELS FROM MAGNETIC RESONANCE IMAGES

    EPA Science Inventory


    Reconstruction of Human Lung Morphology Models from Magnetic Resonance Images
    T. B. Martonen (Experimental Toxicology Division, U.S. EPA, Research Triangle Park, NC 27709) and K. K. Isaacs (School of Public Health, University of North Carolina, Chapel Hill, NC 27514)

  19. MATHEMATICAL ANALYSIS OF PARTICLE TRANSPORT AND DEPOSITION IN HUMAN LUNGS

    EPA Science Inventory

    MATHEMATICAL ANALYSIS OF PARTICLE TRANSPORT AND DEPOSITION IN HUMAN LUNGS. Jung-il Choi*, Center for Environmental Medicine, University of North Carolina, Chapel Hill, NC 27599; C. S. Kim, USEPA National Health and Environmental Effects Research Lab. RTP, NC 27711

    Partic...

  20. MATHEMATICAL ANALYSIS OF PARTICLE TRANSPORT AND DEPOSITION IN HUMAN LUNGS

    EPA Science Inventory

    MATHEMATICAL ANALYSIS OF PARTICLE TRANSPORT AND DEPOSITION IN HUMAN LUNGS. Jung-il Choi*, Center for Environmental Medicine, University of North Carolina, Chapel Hill, NC 27599; C. S. Kim, USEPA National Health and Environmental Effects Research Lab. RTP, NC 27711

    Partic...

  1. Efficient (3)He/(4)He separation in a nanoporous graphenylene membrane.

    PubMed

    Qu, Yuanyuan; Li, Feng; Zhao, Mingwen

    2017-08-16

    Helium-3 is a precious noble gas, which is essential in many advanced technologies such as cryogenics, isotope labeling and nuclear weapons. The current imbalance of (3)He demand and supply shortage leads to the search for an efficient membrane with high performance for (3)He separation. In this study, based on first-principles calculations, we demonstrated that highly efficient (3)He harvesting can be achieved in a nanoporous graphenylene membrane with industrially-acceptable selectivity and permeance. The quantum tunneling effect leads to (3)He harvesting with high efficiency via kinetic sieving. Both the quantum tunneling effect and zero-point energy (ZPE) determine the (3)He/(4)He separation via thermally-driven equilibrium sieving, where the ZPE effect dominates efficient (3)He/(4)He separation between two reservoirs. The quantum effects revealed in this work suggest that the nanoporous graphenylene membrane is promising for efficient (3)He harvesting that can be exploited for industrial applications.

  2. Asbestos fibers in human lung: forensic significance

    SciTech Connect

    Ehrenreich, T.; Selikoff, I.J.

    1981-03-01

    Asbestos is a fibrous mineral which, because of its unique properties, has innumerable applications in many industries and is used in a large variety of consumer products. It has become ubiquitous and is woven, literally and figuratively, into the fabric of our present-day civilization. However, its presence is sometimes unknown and unsuspected by those who are exposed to asbestos by virtue of occupation or environment and inhale its fibers. Exposed workers and even urban dwellers may have a variable lung burden of asbestos fibers. There is indisputable clinical, pathological, experimental and epidemiological proof that, after varying periods of latency, asbestos may cause benign and malignant disease often leading to disability or death. Forensic investigation of suspected asbestos-related deaths includes a life-time occupational history, a complete autopsy, and identification of the asbestos fiber tissue burden. The latter usually requires special procedures.

  3. Selective Inactivity of Pyrazinamide against Tuberculosis in C3HeB/FeJ Mice Is Best Explained by Neutral pH of Caseum.

    PubMed

    Lanoix, Jean-Philippe; Ioerger, Thomas; Ormond, Aimee; Kaya, Firat; Sacchettini, James; Dartois, Véronique; Nuermberger, Eric

    2016-02-01

    Pyrazinamide (PZA) is one of only two sterilizing drugs in the first-line antituberculosis regimen. Its activity is strongly pH dependent; the MIC changes by several orders of magnitude over a range of pH values that may be encountered in various in vivo compartments. We recently reported selective inactivity of PZA in a subset of C3HeB/FeJ mice with large caseous lung lesions. In the present study, we evaluated whether such inactivity was explained by poor penetration of PZA into such lesions or selection of drug-resistant mutants. Despite demonstrating similar dose-proportional PZA exposures in plasma, epithelial lining fluid, and lung lesions, no dose response was observed in a subset of C3HeB/FeJ mice with the highest CFU burden. Although PZA-resistant mutants eventually replaced the susceptible bacilli in BALB/c mice and in C3HeB/FeJ mice with low total CFU burdens, they never exceeded 1% of the total population in nonresponding C3HeB/FeJ mice. The selective inactivity of PZA in large caseous lesions of C3HeB/FeJ mice is best explained by the neutral pH of liquefying caseum.

  4. Modeling of the Nitric Oxide Transport in the Human Lungs

    PubMed Central

    Karamaoun, Cyril; Van Muylem, Alain; Haut, Benoît

    2016-01-01

    In the human lungs, nitric oxide (NO) acts as a bronchodilatator, by relaxing the bronchial smooth muscles and is closely linked to the inflammatory status of the lungs, owing to its antimicrobial activity. Furthermore, the molar fraction of NO in the exhaled air has been shown to be higher for asthmatic patients than for healthy patients. Multiple models have been developed in order to characterize the NO dynamics in the lungs, owing to their complex structure. Indeed, direct measurements in the lungs are difficult and, therefore, these models are valuable tools to interpret experimental data. In this work, a new model of the NO transport in the human lungs is proposed. It belongs to the family of the morphological models and is based on the morphometric model of Weibel (1963). When compared to models published previously, its main new features are the layered representation of the wall of the airways and the possibility to simulate the influence of bronchoconstriction (BC) and of the presence of mucus on the NO transport in lungs. The model is based on a geometrical description of the lungs, at rest and during a respiratory cycle, coupled with transport equations, written in the layers composing an airway wall and in the lumen of the airways. First, it is checked that the model is able to reproduce experimental information available in the literature. Second, the model is used to discuss some features of the NO transport in healthy and unhealthy lungs. The simulation results are analyzed, especially when BC has occurred in the lungs. For instance, it is shown that BC can have a significant influence on the NO transport in the tissues composing an airway wall. It is also shown that the relation between BC and the molar fraction of NO in the exhaled air is complex. Indeed, BC might lead to an increase or to a decrease of this molar fraction, depending on the extent of the BC and on the possible presence of mucus. This should be confirmed experimentally and might

  5. Thyroid hormone metabolism and the developing human lung.

    PubMed

    Hume, R; Richard, K; Kaptein, E; Stanley, E L; Visser, T J; Coughtrie, M W

    2001-05-01

    Thyroid hormones are involved in the regulation of fetal lung development, and maturation is accelerated in animal models by antepartum exposure to raised concentrations of the receptor-active thyroid hormone triiodothyronine and glucocorticoids. It is essential that the nature of the regulation of the spatial and temporal metabolism of iodothyronines in the human fetus and infant is known before effective therapies can be developed to modify human lung maturation. Thyroid hormone bioavailability to the human fetus is regulated in part by enzymatic deiodination and reversible sulfation of iodothyronines, with contributions from other factors such as fetomaternal and fetoamniotic hormone transfers, fetal thyroid gland production, and the activities of plasma membrane transporters mediating uptake of iodothyronines from plasma into tissues. Copyright 2001 S. Karger AG, Basel.

  6. VizieR Online Data Catalog: Cross sections produced by 3He reactions (Murphy+,

    NASA Astrophysics Data System (ADS)

    Murphy, R. J.; Kozlovsky, B.; Share, G. H.

    2017-05-01

    The 3He abundance in impulsive solar energetic particle (SEP) is enhanced up to several orders of magnitude compared to its photospheric value of [3He]/[4He] = 1-3 x 10-4. Interplanetary magnetic field and timing observations suggest that these events are related to solar flares. Observations of 3He in flare-accelerated ions would clarify the relationship between these two phenomena. Energetic 3He interactions in the solar atmosphere produce gamma-ray nuclear-deexcitation lines, both lines that are also produced by protons and α particles and lines that are essentially unique to 3He. Gamma-ray spectroscopy can, therefore, reveal enhanced levels of accelerated 3He. In this paper, we identify all significant deexcitation lines produced by 3He interactions in the solar atmosphere. We evaluate their production cross sections and incorporate them into our nuclear deexcitation-line code. We find that enhanced 3He can affect the entire gamma-ray spectrum. We identify gamma-ray line features for which the yield ratios depend dramatically on the 3He abundance. We determine the accelerated 3He/α ratio by comparing these ratios with flux ratios measured previously from the gamma-ray spectrum obtained by summing the 19 strongest flares observed with the Solar Maximum Mission Gamma-Ray Spectrometer. All six flux ratios investigated show enhanced 3He, confirming earlier suggestions. The 3He/α weighted mean of these new measurements ranges from 0.05 to 0.3 (depending on the assumed accelerated α/proton ratio) and has a <1 x 10-3 probability of being consistent with the photospheric value. With the improved code, we can now exploit the full potential of gamma-ray spectroscopy to establish the relationship between flare-accelerated ions and 3He-rich SEPs. (3 data files).

  7. Activated protooncogenes in human lung tumors from smokers.

    PubMed

    Reynolds, S H; Anna, C K; Brown, K C; Wiest, J S; Beattie, E J; Pero, R W; Iglehart, J D; Anderson, M W

    1991-02-15

    Fourteen primary human lung tumor DNAs from smokers were analyzed for transforming activity by two DNA transfection assays. Activated protooncogenes were detected in 3 of 11 tumor DNAs by the NIH 3T3 focus assay, whereas activated protooncogenes were detected in 11 of 13 tumor DNAs by the NIH 3T3 cotransfection-nude mouse tumorigenicity assay. K- or NRAS genes activated by point mutation at codons 12 or 61 were detected in a large cell carcinoma, a squamous cell carcinoma, and 5 adenocarcinomas. An HRAS oncogene activated by a different mechanism was detected in an epidermoid carcinoma. One adenocarcinoma was found to contain an activated RAF gene. Two unidentified transforming genes were detected in a squamous cell carcinoma DNA and two adenocarcinoma DNAs. Eight of 10 lung adenocarcinomas that had formed metastases at the time of surgery were found to contain RAS oncogenes. No significant increase in metastasis was observed in the lung adenocarcinomas that contained one or more 6-kilobase EcoRI alleles of the LMYC gene. Overall, 12 of 14 (86%) of the lung tumor DNAs from smokers were found to contain activated protooncogenes. RAS oncogenes appear to play a role in the development of metastases in lung adenocarcinomas.

  8. Human lung morphology models for particle deposition studies.

    PubMed

    Martonen, T B; Schroeter, J D; Hwang, D; Fleming, J S; Conway, J H

    2000-01-01

    Knowledge of human lung morphology is of paramount importance in calculating deposition patterns of inhaled particulate matter (PM) to be used in the definition of ambient air quality standards. Due to the inherently complex nature of the branching structure of the airway network, practical assumptions must be made for modeling purposes. The most commonly used mathematical models reported in the literature that describe PM deposition use Weibel's model A morphology. This assumes the airways of the lung to be a symmetric, dichotomously branching system. However, computer simulations of this model, when compared to scintigraphy images, have shown it to lack physiological realism (Martonen et al., 1994a). Therefore, a more physiologically realistic model of the lung is needed to improve the current PM dosimetry models. Herein, a morphological model is presented that is based on laboratory data from planar gamma camera and single-photon emission computed tomography (SPECT) images. Key elements of this model include: The parenchymal wall of the lung is defined in mathematical terms, the whole lung is divided into distinct left and right components, a set of branching angles is derived from experimental measurements, and the branching network is confined within the discrete left and right components (i.e., there is no overlapping of airways). In future work, this new, more physiologically realistic morphological model can be used to calculate PM deposition patterns for risk assessment protocols.

  9. A possible in situ 3H and 3He source in Earth's interior: an alternative explanation of origin of 3He in deep Earth.

    PubMed

    Jiang, Songsheng; Liu, Jing; He, Ming

    2010-07-01

    Origin of (3)He in the Earth is a mystery. Lacking a production mechanism, scientists assume (3)He was trapped in the Earth, when the Earth was formed. In contrast to this assumption, we have found (3)He and (3)H concentrations in excess of the atmospheric values in the deep waters of the volcanic Lakes Pavin (France), Laacher (Germany) and Nemrut (Turkey). This paper reports the result of finding (3)H in these three volcanic lakes that appear to originate from the mantle. Because (3)H has a half-life of 12.3 years, this (3)H and the resulting (3)He must have formed recently in the mantle and not be part of a primordial reservoir. The nuclear reactions that generate tritium might be a source of "missing" energy in the interior of the Earth.

  10. A possible in situ 3H and 3He source in Earth's interior: an alternative explanation of origin of 3He in deep Earth

    NASA Astrophysics Data System (ADS)

    Jiang, Songsheng; Liu, Jing; He, Ming

    2010-07-01

    Origin of 3He in the Earth is a mystery. Lacking a production mechanism, scientists assume 3He was trapped in the Earth, when the Earth was formed. In contrast to this assumption, we have found 3He and 3H concentrations in excess of the atmospheric values in the deep waters of the volcanic Lakes Pavin (France), Laacher (Germany) and Nemrut (Turkey). This paper reports the result of finding 3H in these three volcanic lakes that appear to originate from the mantle. Because 3H has a half-life of 12.3 years, this 3H and the resulting 3He must have formed recently in the mantle and not be part of a primordial reservoir. The nuclear reactions that generate tritium might be a source of “missing” energy in the interior of the Earth.

  11. Near threshold two meson production with the pd→3Heπ+π- and pd→3HeK+K- reactions

    NASA Astrophysics Data System (ADS)

    Bellemann, F.; Berg, A.; Bisplinghoff, J.; Bohlscheid, G.; Ernst, J.; Henrich, C.; Hinterberger, F.; Ibald, R.; Jahn, R.; Jarczyk, L.; Joosten, R.; Kozela, A.; Machner, H.; Magiera, A.; Maschuw, R.; Mayer-Kuckuk, T.; Mertler, G.; Munkel, J.; von Neumann-Cosel, P.; Rosendaal, D.; von Rossen, P.; Schnitker, H.; Scho, K.; Smyrski, J.; Strzalkowski, A.; Tölle, R.; Wilkin, C.

    2000-06-01

    Near threshold two meson production via the reactions pd→3Heπ+π- and pd→3HeK+K- was measured kinematically complete with the MOMO experiment at COSY. The obtained two pion invariant mass spectra and angular distributions depict a remarkable deviation from phase space. The two kaon data are consistent with phase space topped by a clear signal of the φ meson.

  12. Thermodynamic properties of liquid 3He- 4He mixtures at zero pressure for temperatures below 250 mK and 3He concentrations below 8%

    NASA Astrophysics Data System (ADS)

    Kuerten, J. G. M.; Castelijns, C. A. M.; de Waele, A. T. A. M.; Gijsman, H. M.

    We calculated the thermodynamic quantities of dilute liquid 3He- 4He mixtures, starting from experimental values of the specific heat and the osmotic pressure. The calculations are confined to temperatures below 250 mK and 3He concentrations below 8% at zero pressure. Some results are especially useful for dilution refrigeration. Contrary to the calculations previously performed by Radebaugh, our results are in good agreement with the experimental date on both the osmotic pressure and the osmotic enthalpy.

  13. Smoking increases carcinogenic polycyclic aromatic hydrocarbons in human lung tissue.

    PubMed

    Goldman, R; Enewold, L; Pellizzari, E; Beach, J B; Bowman, E D; Krishnan, S S; Shields, P G

    2001-09-01

    Tobacco smoke is a major source of human exposure to polycyclic aromatic hydrocarbons (PAHs). The concentration of PAHs in lung tissue would reflect an individual's dose, and its variation could perhaps reflect cancer risk. Eleven PAHs were measured in 70 lung tissue samples from cancer-free autopsy donors by gas chromatography-mass spectrometry. There were 37 smokers and 33 nonsmokers as estimated by serum cotinine concentration. The sum of PAH concentrations was higher in smokers (P = 0.01), and there was a dose-response relationship for greater smoking (P < 0.01). Smoking increased the concentration of five PAHs including benzo(a)pyrene, which increased approximately 2-fold. The risk for increasing carcinogenic PAHs (odds ratio, 8.20; 95% confidence interval, 2.39-28.09) was 3-fold compared with noncarcinogenic PAHs (odds ratio, 2.61; 95% confidence interval, 0.75-9.12). A higher concentration of PAHs was detected in the lung tissue of males, although the estimated smoking was similar in males and females. Race was not associated with PAH concentrations overall, but PAH concentrations appeared to be higher in African-American males than in any other group. Age was weakly correlated with an increase in fluoranthene and pyrene. The measurement of PAHs in human lung tissue can be used to estimate the actual dose to the target organ.

  14. Discrimination and quantification of autofluorescence spectra of human lung cells

    NASA Astrophysics Data System (ADS)

    Rahmani, Mahya; Khani, Mohammad Mehdi; Khazaei Koohpar, Zeinab; Molik, Paria

    2016-10-01

    To study laser-induced autofluorescence spectroscopy of the human lung cell line, we evaluated the native fluorescence properties of cancer QU-DB and normal MRC-5 human lung cells during continuous exposure to 405 nm laser light. Two emission bands centered at ~470 nm and ~560 nm were observed. These peaks are most likely attributable to mitochondrial fluorescent reduced nicotinamide adenine dinucleotide and riboflavin fluorophores, respectively. This article highlights lung cell autofluorescence characterization and signal discrimination by collective investigation of different spectral features. The absolute intensity, the spectral shape factor or redox ratio, the full width of half-maximum and the full width of quarter maximum was evaluated. Moreover, the intensity ratio, the area under the peak and the area ratio as a contrast factor for normal and cancerous cells were also calculated. Among all these features it seems that the contrast factor precisely and significantly discriminates the spectral differences of normal and cancerous lung cells. On the other hand, the relative quantum yield for both cell types were found by comparing the quantum yield of an unknown compound with known fluorescein sodium as a reference solution.

  15. Aerosol Deposition in the Human Lung in Reduced Gravity

    PubMed Central

    2014-01-01

    Abstract The deposition of aerosol in the human lung occurs mainly through a combination of inertial impaction, gravitational sedimentation, and diffusion. For 0.5- to 5-μm-diameter particles and resting breathing conditions, the primary mechanism of deposition in the intrathoracic airways is sedimentation, and therefore the fate of these particles is markedly affected by gravity. Studies of aerosol deposition in altered gravity have mostly been performed in humans during parabolic flights in both microgravity (μG) and hypergravity (∼1.6G), where both total deposition during continuous aerosol mouth breathing and regional deposition using aerosol bolus inhalations were performed with 0.5- to 3-μm particles. Although total deposition increased with increasing gravity level, only peripheral deposition as measured by aerosol bolus inhalations was strongly dependent on gravity, with central deposition (lung depth<200 mL) being similar between gravity levels. More recently, the spatial distribution of coarse particles (mass median aerodynamic diameter≈5 μm) deposited in the human lung was assessed using planar gamma scintigraphy. The absence of gravity caused a smaller portion of 5-μm particles to deposit in the lung periphery than in the central region, where deposition occurred mainly in the airways. Indeed, 5-μm-diameter particles deposit either by inertial impaction, a mechanism most efficient in the large and medium-sized airways, or by gravitational sedimentation, which is most efficient in the distal lung. On the contrary, for fine particles (∼1 μm), both aerosol bolus inhalations and studies in small animals suggest that particles deposit more peripherally in μG than in 1G, beyond the reach of the mucociliary clearance system. PMID:24870702

  16. Aerosol deposition in the human lung in reduced gravity.

    PubMed

    Darquenne, Chantal

    2014-06-01

    The deposition of aerosol in the human lung occurs mainly through a combination of inertial impaction, gravitational sedimentation, and diffusion. For 0.5- to 5-μm-diameter particles and resting breathing conditions, the primary mechanism of deposition in the intrathoracic airways is sedimentation, and therefore the fate of these particles is markedly affected by gravity. Studies of aerosol deposition in altered gravity have mostly been performed in humans during parabolic flights in both microgravity (μG) and hypergravity (~1.6G), where both total deposition during continuous aerosol mouth breathing and regional deposition using aerosol bolus inhalations were performed with 0.5- to 3-μm particles. Although total deposition increased with increasing gravity level, only peripheral deposition as measured by aerosol bolus inhalations was strongly dependent on gravity, with central deposition (lung depth<200 mL) being similar between gravity levels. More recently, the spatial distribution of coarse particles (mass median aerodynamic diameter≈5 μm) deposited in the human lung was assessed using planar gamma scintigraphy. The absence of gravity caused a smaller portion of 5-μm particles to deposit in the lung periphery than in the central region, where deposition occurred mainly in the airways. Indeed, 5-μm-diameter particles deposit either by inertial impaction, a mechanism most efficient in the large and medium-sized airways, or by gravitational sedimentation, which is most efficient in the distal lung. On the contrary, for fine particles (~1 μm), both aerosol bolus inhalations and studies in small animals suggest that particles deposit more peripherally in μG than in 1G, beyond the reach of the mucociliary clearance system.

  17. Metastability exchange optical pumping of 3He gas up to hundreds of millibars at 4.7 Tesla

    NASA Astrophysics Data System (ADS)

    Nikiel-Osuchowska, Anna; Collier, Guilhem; Głowacz, Bartosz; Pałasz, Tadeusz; Olejniczak, Zbigniew; Wȩglarz, Władysław P.; Tastevin, Geneviève; Nacher, Pierre-Jean; Dohnalik, Tomasz

    2013-09-01

    Metastability exchange optical pumping (MEOP) is experimentally investigated in 3He at 4.7 T, at room temperature and for gas pressures ranging from 1 to 267 mbar. The 23S-23P transition at 1083 nm is used for optical pumping and for detection of the laser-induced orientation of 3He atoms in the rf discharge plasma. The collisional broadening rate is measured (12.0 ± 0.4 MHz mbar-1 FHWM) and taken into account for accurate absorption-based measurements of both nuclear polarization in the ground state and atom number density in the metastable 23S state. The results lay the ground for a comprehensive assessment of the efficiency of MEOP, by comparison with achievements at lower field (1 mT-2 T) over an extended range of operating conditions. Stronger hyperfine decoupling in the optically pumped 23S state is observed to systematically lead to slower build-up of 3He orientation in the ground state, as expected. The nuclear polarizations obtained at 4.7 T still decrease at high pressure but in a less dramatic way than observed at 2 T in the same sealed glass cells. To date, thanks to the linear increase in gas density, they correspond to the highest nuclear magnetizations achieved by MEOP in pure 3He gas. The improved efficiency puts less demanding requirements for compression stages in polarized gas production systems and makes high-field MEOP particularly attractive for magnetic resonance imaging of the lungs, for instance.

  18. A 3He Cryostat for Scientific Measurements in Pulsed High Magnetic Fields

    NASA Astrophysics Data System (ADS)

    Wang, Shaoliang; Li, Liang; Liu, Mengyu; Zuo, Huakun; Peng, Tao

    A top loading 3He cryostat has been developed for scientific experiments with a 60 T pulsed magnetic field facility at Wuhan National High Magnetic Field Center. The cryostat consists of a 4He bath cryostat, a 3He insert and a closed circulation system for 3He gas handling. To eliminate the eddy current heating during the pulse, the tail of the 3He insert with a vacuum space at the bottom is made from fiberglass tubing coated with epoxy. The 3He bath is separated from the 4He bath with the vacuum space. The 4He bath cryostat provides cooling power to condense 3He gas by a neck tube on top of the tail. Experimental results have shown that the sample can be cooled down to 385 mK and kept cold for more than 150 second by one-shot cooling, which is sufficiently long for an experiment in a pulsed high magnetic field.

  19. Effect of ^3He impurity on the supersolid transition of ^4He

    NASA Astrophysics Data System (ADS)

    Kim, E.; Xia, J. S.; West, J. T.; Lin, X.; Chan, M. H. W.

    2007-03-01

    The supersolid phase of ^4He was reported by a series of torsional oscillator experiments [1]. One of the most striking features of the supersolid transition is the intriguing ^3He impurity effect. The addition of an extremely small amount of ^3He impurity broadens the transition and enhances the transition temperature Tc. This effect is very different from that in helium film and that in `bulk' superfluid helium. We have studied the influence of ^3He impurity on the supersolid transition systematically by progressively diluting isotopically-pure ^4He (^3He impurity less than 2ppb) with ^3He. The transition temperature is monotonically enhanced with increasing ^3He concentration and the supersolid fraction shows a broad maximum around 0.2 ppm. [1] E. Kim and M. H. W. Chan, Science 305, 1941 (2004); Nature 425, 227 (2004); J. Low Temp. Phys. 138, 859 (2005); Phys. Rev. Lett. 97, 115302 (2006).

  20. Comparative Pathobiology of Environmentally Induced Lung Cancers in Humans and Rodents

    PubMed Central

    Pandiri, Arun

    2014-01-01

    Lung cancer is the number one cause of cancer-related deaths in humans worldwide. Environmental factors play an important role in the epidemiology of these cancers. Rodents are the most common experimental model to study human lung cancers and are frequently used in bioassays to identify environmental exposure hazards associated with lung cancer. Lung tumors in rodents are common, particularly in certain strains of mice. Rodent lung tumors are predominantly bronchioloalveolar carcinomas and usually follow a progressive continuum of hyperplasia to adenoma to carcinoma. Human lung cancers are phenotypically more diverse and broadly constitute 2 types: small cell lung cancers or non-small cell lung cancers. Rodent lung tumors resulting from exposure to environmental agents are comparable to certain adenocarcinomas that are a subset of human non-small cell lung cancers. Human pulmonary carcinomas differ from rodent lung tumors by exhibiting greater morphologic heterogeneity (encompassing squamous cell, neuroendocrine, mucinous, sarcomatoid, and multiple cell combinations), higher metastatic rate, higher stromal response, aggressive clinical behavior, and lack of a clear continuum of proliferative lesions. In spite of these differences, rodent lung tumors recapitulate several fundamental aspects of human lung tumor biology at the morphologic and molecular level especially in lung cancers resulting from exposure to environmental carcinogens. PMID:25351923

  1. 3He immersion cell for ultralow temperature study of amorphous solids

    NASA Astrophysics Data System (ADS)

    Rogge, Sven; Natelson, Douglas; Osheroff, D. D.

    1997-04-01

    We have constructed a 3He immersion cell for dielectric measurements of insulating amorphous solids in a nuclear demagnetization cryostat at temperatures between 500 μK and 150 mK. The samples are directly immersed in 3He with two heat exchangers per electrode which are thermally isolated from each other and have a very low capacitance to ground. The cell incorporates a 195Pt pulsed NMR thermometer with a novel superconducting magnet and a 3He viscometer for calibration.

  2. Transport in very dilute solutions of 3He in superfluid 4He

    NASA Astrophysics Data System (ADS)

    Baym, Gordon; Beck, D. H.; Pethick, C. J.

    2013-07-01

    Motivated by a proposed experimental search for the electric dipole moment of the neutron (nEDM) utilizing neutron-3He capture in a dilute solution of 3He in superfluid 4He, we derive the transport properties of dilute solutions in the regime where the 3He are classically distributed and rapid 3He-3He scatterings keep the 3He in equilibrium. Our microscopic framework takes into account phonon-phonon, phonon-3He, and 3He-3He scatterings. We then apply these calculations to measurements by Rosenbaum [J. Low Temp. Phys.JLTPAC0022-229110.1007/BF00655864 16, 131 (1974)] and by Lamoreaux [Europhys. Lett.EULEEJ0295-507510.1209/epl/i2002-00408-4 58, 718 (2002)] of dilute solutions in the presence of a heat flow. We find satisfactory agreement of theory with the data, serving to confirm our understanding of the microscopics of the helium in the future nEDM experiment.

  3. Cluster folding model analysis of 3He elastic and inelastic scattering from 12C

    NASA Astrophysics Data System (ADS)

    Khallaf, S. A. E.; Nossair, A. M. A.; Ebrahim, A. A.; Ebraheem, Awad A.

    2003-02-01

    Angular distributions of differential cross sections for the 12C( 3He, 3He) 12C, 12C( 3He, 3He) 12C ∗ reactions at E=72 MeV have been analyzed with a double folding cluster model DFC based on five sets of the effective N-N interaction of Gaussian form with different parameters. The transition to the (2 +; 4.44 MeV) state in 12C is studied and the deformation length δ2 is extracted. It is found that the extracted deformation length is sensitive to the nuclear model used and it is similar to the corresponding value found in the literature.

  4. Development of a thermodynamic model for a cold cycle 3He-4He dilution refrigerator

    NASA Astrophysics Data System (ADS)

    Mueller, B. W.; Miller, F. K.

    2016-10-01

    A thermodynamic model of a 3He-4He cold cycle dilution refrigerator with no actively-driven mechanical components is developed and investigated. The refrigerator employs a reversible superfluid magnetic pump, passive check valves, a phase separation chamber, and a series of recuperative heat exchangers to continuously circulate 3He-4He and maintain a 3He concentration gradient across the mixing chamber. The model predicts cooling power and mixing chamber temperature for a range of design and operating parameters, allowing an evaluation of feasibility for potential 3He-4He cold cycle dilution refrigerator prototype designs. Model simulations for a prototype refrigerator design are presented.

  5. Ion Implantation of 3He in Tantalum for Use in a Low Energy Deuteron Polarization Analyzer

    DTIC Science & Technology

    1994-01-01

    depends on aAzz2 where a and A are the cross section and analyzing power, respectively. The cross section of the 3He ( d ,p) reaction below 978 keV appears...protons from the 3He ( d ,p) 4He reaction would be more visible. The statistics were poor, but a definite implant in all the targets on the order of 1017 3He ...accelerator and a polarimeter using the 3He ( d ,p)4 He reaction . (Figure 8) During the experiment Pzz was measured at the beginning, and twice during the tests

  6. The influence of restricted geometry of diamagnetic nanoporous media on 3He relaxation

    NASA Astrophysics Data System (ADS)

    Alakshin, E. M.; Gazizulin, R. R.; Zakharov, M. Yu.; Klochkov, A. V.; Morozov, E. V.; Salikhov, T. M.; Safin, T. R.; Safiullin, K. R.; Tagirov, M. S.; Shabanova, O. B.

    2015-01-01

    This is an experimental study of the spin kinetics of 3He in contact with diamagnetic samples of inverse opals SiO2, and LaF3 nanopowder. It is demonstrated that the nuclear magnetic relaxation of the absorbed 3He occurs due to the modulation of dipole-dipole interaction by the quantum motion in the two-dimensional film. It is found that the relaxation of liquid 3He occurs through a spin diffusion to the absorption layer, and that the restricted geometry of diamagnetic nanoporous media has an influence on the 3He relaxation.

  7. Human Lung Small Airway-on-a-Chip Protocol.

    PubMed

    Benam, Kambez H; Mazur, Marc; Choe, Youngjae; Ferrante, Thomas C; Novak, Richard; Ingber, Donald E

    2017-01-01

    Organs-on-chips are microfluidic cell culture devices created using microchip manufacturing techniques that contain hollow microchannels lined by living cells, which recreate specialized tissue-tissue interfaces, physical microenvironments, and vascular perfusion necessary to recapitulate organ-level physiology in vitro. Here we describe a protocol for fabrication, culture, and operation of a human lung "small airway-on-a-chip," which contains a differentiated, mucociliary bronchiolar epithelium exposed to air and an underlying microvascular endothelium that experiences fluid flow. First, microengineering is used to fabricate a multilayered microfluidic device that contains two parallel elastomeric microchannels separated by a thin rigid porous membrane; this requires less than 1 day to complete. Next, primary human airway bronchiolar epithelial cells isolated from healthy normal donors or patients with respiratory disease are cultured on the porous membrane within one microchannel while lung microvascular endothelial cells are cultured on the opposite side of the same membrane in the second channel to create a mucociliated epithelium-endothelium interface; this process take about 4-6 weeks to complete. Finally, culture medium containing neutrophils isolated from fresh whole human blood are flowed through the microvascular channel of the device to enable real-time analysis of capture and recruitment of circulating leukocytes by endothelium under physiological shear; this step requires less than 1 day to complete. The small airway-on-a-chip represents a new microfluidic tool to model complex and dynamic inflammatory responses of healthy and diseased lungs in vitro.

  8. C-reactive protein modulates human lung fibroblast migration.

    PubMed

    Kikuchi, Kazuhiko; Kohyama, Tadashi; Yamauchi, Yasuhiro; Kato, Jun; Takami, Kazutaka; Okazaki, Hitoshi; Desaki, Masashi; Nagase, Takahide; Rennard, Stephen I; Takizawa, Hajime

    2009-02-01

    C-reactive protein (CRP) has been classically used as a marker of inflammation. The aim of this study was to investigate the effect of CRP on migration of human fetal lung fibroblasts (HFL-1) to human plasma fibronectin (HFn). Using the blindwell chamber technique, CRP inhibited HFL-1 migration in a dose-dependent fashion (at 1 microg/mL, inhibition: 32.5% +/- 7.1%; P < .05). Western blot analysis showed that CRP inhibited the p38 mitogen-activated protein kinase (MAPK) activity in the presence of HFn. Moreover, the MAPK inhibitors SB202190 (25 microM) and SB203580 (25 microM) inhibited HFn-induced cell migration, suggesting an important role of p38 MAPK in HFn-induced migration. Taken together, these results suggest that the inhibitory effect of CRP is mediated by blocking MAPK. In summary, this study demonstrates that CRP directly modulates human lung fibroblasts migration. Thus, CRP may contribute to regulation of wound healing and may be endogenous antifibrotic factor acting on lung fibrosis.

  9. Human lung small-cell carcinoma contains bombesin.

    PubMed Central

    Erisman, M D; Linnoila, R I; Hernandez, O; DiAugustine, R P; Lazarus, L H

    1982-01-01

    The presence of immunoreactive bombesin in a human lung small-cell carcinoma grown in nude mice was established by several criteria: (i) Radioimmunoassay of tissue extracts for bombesin revealed approximately 6.5 pmol/g of tissue; (ii) bombesin was found in 12-14% of the tumor cells by immunohistochemical localization; (iii) gel filtration of small-cell carcinoma extract on Sephadex G-75 and Bio-Gel P-4 gave only a single peak of immunoreactivity, which occurred at the elution volume of bombesin; and (iv) reverse-phase HPLC of acid-solubilized extracts separated the immunoreactive material into three discrete peaks, one of which eluted with a retention time identical to that of synthetic bombesin. The presence of bombesin may represent the ectopic expression of this peptide in small-cell carcinoma, because immunoreactive bombesin was found in human fetal and neonatal lung but apparently not in adult lung tissue [Wharton, J., Polak, J. M., Bloom, S. R., Ghatei, M. A., Solcia, E., Brown, M. R. & Pearse, A. G. E. (1978) Nature (London) 273, 769-770]. The immunoreactive bombesin previously found in mammalian tissues is considerably larger than amphibian bombesin; these data substantiate the presence of a mammalian form of bombesin in a human tumor that may have a structure similar to that of the amphibian peptide. Images PMID:6285381

  10. Radiation-enhanced lung cancer progression in a transgenic mouse model of lung cancer is predictive of outcomes in human lung and breast cancer.

    PubMed

    Delgado, Oliver; Batten, Kimberly G; Richardson, James A; Xie, Xian-Jin; Gazdar, Adi F; Kaisani, Aadil A; Girard, Luc; Behrens, Carmen; Suraokar, Milind; Fasciani, Gail; Wright, Woodring E; Story, Michael D; Wistuba, Ignacio I; Minna, John D; Shay, Jerry W

    2014-03-15

    Carcinogenesis is an adaptive process between nascent tumor cells and their microenvironment, including the modification of inflammatory responses from antitumorigenic to protumorigenic. Radiation exposure can stimulate inflammatory responses that inhibit or promote carcinogenesis. The purpose of this study is to determine the impact of radiation exposure on lung cancer progression in vivo and assess the relevance of this knowledge to human carcinogenesis. K-ras(LA1) mice were irradiated with various doses and dose regimens and then monitored until death. Microarray analyses were performed using Illumina BeadChips on whole lung tissue 70 days after irradiation with a fractionated or acute dose of radiation and compared with age-matched unirradiated controls. Unique group classifiers were derived by comparative genomic analysis of three experimental cohorts. Survival analyses were performed using principal component analysis and k-means clustering on three lung adenocarcinoma, three breast adenocarcinoma, and two lung squamous carcinoma annotated microarray datasets. Radiation exposure accelerates lung cancer progression in the K-ras(LA1) lung cancer mouse model with dose fractionation being more permissive for cancer progression. A nonrandom inflammatory signature associated with this progression was elicited from whole lung tissue containing only benign lesions and predicts human lung and breast cancer patient survival across multiple datasets. Immunohistochemical analyses suggest that tumor cells drive predictive signature. These results demonstrate that radiation exposure can cooperate with benign lesions in a transgenic model of cancer by affecting inflammatory pathways, and that clinically relevant similarities exist between human lung and breast carcinogenesis. ©2014 AACR.

  11. Activation of proto-oncogenes in human and mouse lung tumors

    SciTech Connect

    Reynolds, S.H.; Anderson, M.W. )

    1991-06-01

    Lung cancer is a leading cause of cancer-related deaths in several nations. Epidemiological studies have indicated that 85% of all lung cancer deaths and 30% of all cancer deaths in the US are associated with tobacco smoking. Various chemicals in tobacco smoke are thought to react with DNA and to ultimately yield heritable mutations. In an effort to understand the molecular mechanisms involved in lung tumorigenesis, the authors have analyzed proto-oncogene activation in a series of human lung tumors from smokers and spontaneously occurring and chemically induced lung tumors in mice. Approximately 86% of the human lung tumors and > 90% of the mouse lung tumors were found to contain activated oncogenes. ras Oncogenes activated by point mutations were detected in many of the human lung adenocarcinomas and virtually all of the mouse lung adenomas and adenocarcinomas. The mutation profiles of the activated K-ras genes detected in the chemically induced mouse lung tumors suggest that the observed mutations result from genotoxic effects of the chemicals. Comparison of the K-ras mutations observed in the human lung adenocarcinomas with mutation profiles observed in the mouse lung tumors suggest that bulky hydrophobic DNA adducts may be responsible for the majority of the mutations observed in the activated human K-ras genes. Other data indicate that approximately 20% of human lung tumors contain potentially novel transforming genes that may also be targets for mutagens in cigarette smoke.

  12. Sex-specific differences in hyperoxic lung injury in mice: Implications for acute and chronic lung disease in humans

    SciTech Connect

    Lingappan, Krithika; Jiang, Weiwu; Wang, Lihua; Couroucli, Xanthi I.; Barrios, Roberto; Moorthy, Bhagavatula

    2013-10-15

    Sex-specific differences in pulmonary morbidity in humans are well documented. Hyperoxia contributes to lung injury in experimental animals and humans. The mechanisms responsible for sex differences in the susceptibility towards hyperoxic lung injury remain largely unknown. In this investigation, we tested the hypothesis that mice will display sex-specific differences in hyperoxic lung injury. Eight week-old male and female mice (C57BL/6J) were exposed to 72 h of hyperoxia (FiO{sub 2} > 0.95). After exposure to hyperoxia, lung injury, levels of 8-iso-prostaglandin F{sub 2} alpha (8-iso-PGF 2α) (LC–MS/MS), apoptosis (TUNEL) and inflammatory markers (suspension bead array) were determined. Cytochrome P450 (CYP)1A expression in the lung was assessed using immunohistochemistry and western blotting. After exposure to hyperoxia, males showed greater lung injury, neutrophil infiltration and apoptosis, compared to air-breathing controls than females. Pulmonary 8-iso-PGF 2α levels were higher in males than females after hyperoxia exposure. Sexually dimorphic increases in levels of IL-6 (F > M) and VEGF (M > F) in the lungs were also observed. CYP1A1 expression in the lung was higher in female mice compared to males under hyperoxic conditions. Overall, our results support the hypothesis that male mice are more susceptible than females to hyperoxic lung injury and that differences in inflammatory and oxidative stress markers contribute to these sex-specific dimorphic effects. In conclusion, this paper describes the establishment of an animal model that shows sex differences in hyperoxic lung injury in a temporal manner and thus has important implications for lung diseases mediated by hyperoxia in humans. - Highlights: • Male mice were more susceptible to hyperoxic lung injury than females. • Sex differences in inflammatory markers were observed. • CYP1A expression was higher in females after hyperoxia exposure.

  13. Steady-state free precession with hyperpolarized 3He: Experiments and theory

    NASA Astrophysics Data System (ADS)

    Wild, Jim M.; Teh, Kevin; Woodhouse, Neil; Paley, Martyn N. J.; Fichele, Stan; de Zanche, Nicola; Kasuboski, Larry

    2006-11-01

    The magnetization response of hyperpolarized 3He gas to a steady-state free precession (SSFP) sequence was simulated using matrix product operators. The simulations included the effects of flip angle ( α), sequence timings, resonant frequency, gas diffusion coefficient, imaging gradients, T1 and T2. Experiments performed at 1.5 T, on gas phantoms and with healthy human subjects, confirm the predicted theory, and indicate increased SNR with SSFP through use of higher flip angles when compared to optimized spoiled gradient echo (SPGR). Simulations and experiments show some compromise to the SNR and some point spread function broadening at high α due to the incomplete refocusing of transverse magnetization, caused by diffusion dephasing from the readout gradient. Mixing of gas polarization levels by diffusion between slices is also identified as a source of signal loss in SSFP at higher α through incomplete refocusing. Nevertheless, in the sample experiments, a SSFP sequence with an optimized flip angle of α = 20°, and 128 sequential phase encoding views, showed a higher SNR when compared to SPGR ( α = 7.2°) with the same bandwidth. Some of the gas sample experiments demonstrated a transient signal response that deviates from theory in the initial phase. This was identified as being caused by radiation damping interactions between the large initial transverse magnetization and the high quality factor ( Q = 250) birdcage resonator. In 3He NMR experiments, performed without imaging gradients, diffusion dephasing can be mitigated, and the effective T2 is relatively long (⩾1 s). Under these circumstances the SSFP sequence behaves like a CPMG sequence with sin( α/2) weighting of SNR. Experiments and simulations were also performed to characterize the off-resonance behaviour of the SSFP HP 3He signal. Characteristic banding artifacts due to off-resonance harmonic beating were observed in some of the in vivo SSFP images, for instance in axial slices close to the

  14. Steady-state free precession with hyperpolarized 3He: experiments and theory.

    PubMed

    Wild, Jim M; Teh, Kevin; Woodhouse, Neil; Paley, Martyn N J; Fichele, Stan; de Zanche, Nicola; Kasuboski, Larry

    2006-11-01

    The magnetization response of hyperpolarized 3He gas to a steady-state free precession (SSFP) sequence was simulated using matrix product operators. The simulations included the effects of flip angle (alpha), sequence timings, resonant frequency, gas diffusion coefficient, imaging gradients, T1 and T2. Experiments performed at 1.5 T, on gas phantoms and with healthy human subjects, confirm the predicted theory, and indicate increased SNR with SSFP through use of higher flip angles when compared to optimized spoiled gradient echo (SPGR). Simulations and experiments show some compromise to the SNR and some point spread function broadening at high alpha due to the incomplete refocusing of transverse magnetization, caused by diffusion dephasing from the readout gradient. Mixing of gas polarization levels by diffusion between slices is also identified as a source of signal loss in SSFP at higher alpha through incomplete refocusing. Nevertheless, in the sample experiments, a SSFP sequence with an optimized flip angle of alpha=20 degrees, and 128 sequential phase encoding views, showed a higher SNR when compared to SPGR (alpha=7.2 degrees) with the same bandwidth. Some of the gas sample experiments demonstrated a transient signal response that deviates from theory in the initial phase. This was identified as being caused by radiation damping interactions between the large initial transverse magnetization and the high quality factor (Q=250) birdcage resonator. In 3He NMR experiments, performed without imaging gradients, diffusion dephasing can be mitigated, and the effective T2 is relatively long (1 s). Under these circumstances the SSFP sequence behaves like a CPMG sequence with sinalpha/2 weighting of SNR. Experiments and simulations were also performed to characterize the off-resonance behaviour of the SSFP HP 3He signal. Characteristic banding artifacts due to off-resonance harmonic beating were observed in some of the in vivo SSFP images, for instance in axial slices

  15. Expression of Formyl-peptide Receptors in Human Lung Carcinoma.

    PubMed

    Cattaneo, Fabio; Guerra, Germano; Parisi, Melania; Lucariello, Angela; De Luca, Antonio; De Rosa, Nicolina; Mazzarella, Gennaro; Bianco, Andrea; Ammendola, Rosario

    2015-05-01

    Formyl-peptide receptors (FPRs) are expressed in several tissues and cell types. The identification of markers involved in cell growth may further allow for molecular profiling of lung cancer. We investigated the possible role of FPRs as molecular markers in several types of lung carcinomas which is the main cause of cancer death worldwide. Tumor tissue samples were collected from six patients affected by lung cancer. Biopsies were analyzed for expression of FPR isoforms both in tumoral and peritumoral tissue by real-time polymerase chain reaction (PCR), western blot and immunofluorescence. Real-time PCR, western blot and immunofluorescence analyses showed that FPR expression is lower in types of human lung cancer tissues when compared to the surrounding peritumoral tissues. The study of the mechanistic basis for the control of FPR expression in normal peritumoral versus tumoral tissues could provide the basis for new diagnostic and therapeutic interventions. Copyright© 2015 International Institute of Anticancer Research (Dr. John G. Delinassios), All rights reserved.

  16. Proteogenomic Analysis of Human Chromosome 9-Encoded Genes from Human Samples and Lung Cancer Tissues

    PubMed Central

    Ahn, Jung-Mo; Kim, Min-Sik; Kim, Yong-In; Jeong, Seul-Ki; Lee, Hyoung-Joo; Lee, Sun Hee; Paik, Young-Ki; Pandey, Akhilesh; Cho, Je-Yoel

    2014-01-01

    The Chromosome-centric Human Proteome Project (C-HPP) was recently initiated as an international collaborative effort. Our team adopted chromosome 9 (Chr 9) and performed a bioinformatics and proteogenomic analysis to catalog Chr 9-encoded proteins from normal tissues, lung cancer cell lines and lung cancer tissues. Approximately 74.7% of the Chr 9 genes of the human genome were identified, which included approximately 28% of missing proteins (46 of 162) on Chr 9 compared with the list of missing proteins from the neXtProt master table (2013-09). In addition, we performed a comparative proteomics analysis between normal lung and lung cancer tissues. Based on the data analysis, 15 proteins from Chr 9 were detected only in lung cancer tissues. Finally, we conducted a proteogenomic analysis to discover Chr 9-residing single nucleotide polymorphisms (SNP) and mutations described in the COSMIC cancer mutation database. We identified 21 SNPs and 4 mutations containing peptides on Chr 9 from normal human cells/tissues and lung cancer cell lines, respectively. In summary, this study provides valuable information of the human proteome for the scientific community as part of C-HPP. The mass spectrometry proteomics data have been deposited to the ProteomeXchange Consortium with the data set identifier PXD. PMID:24274035

  17. Temperature Measurements of Fusion Plasmas Produced by Laser-Irradiated D2-3 He or CD4-3 He Clustering Gases

    NASA Astrophysics Data System (ADS)

    Bang, W.; Ditmire, T.; Quevedo, H.; Dyer, G.; Bernstein, A. C.; Donovan, M.; Gaul, E.; Barbui, M.; Bonasera, A.; Hagel, K.; Natowitz, J. B.

    2014-10-01

    We report on experiments in which a mixture of D2 or CD4 clusters and 3He gas was irradiated by a petawatt-laser pulse, generating nuclear fusion reactions such as D(d, 3He) n, D(d, t) p , and 3He(d, p)4He. We measured the yields of fusion neutrons and protons from these reactions and found them to agree with yields based on a simple cylindrical plasma model. The plasma temperature was determined by two different methods. In the first, it was derived from time-of-flight data of deuterium ions ejected from exploding D2 or CD4 clusters. In the second, it was measured from the ratio of neutron yield to proton yield from D(d, 3He) n and 3He(d, p)4He reactions, respectively. The temperatures determined by these two methods agree well, indicating (i) the ion energy distribution is not significantly distorted when ions travel in the disassembling plasma; (ii) the kinetic energy of deuterium ions, especially the hottest part responsible for nuclear fusion, is well described by a near-Maxwellian distribution.

  18. Type 3 solar radio bursts and 3HE-rich events

    NASA Technical Reports Server (NTRS)

    Reames, D. V.; Stone, R. G.

    1985-01-01

    The kilometric radio data for 3He-rich events during the 1979 to 82 time period were investigated. Type 3 bursts are present for each event as expected from the prevous electron 3He-event association. A list of identified solar events is presented.

  19. Fusion product studies via fast ion D-D and D-3He fusion on JET

    NASA Astrophysics Data System (ADS)

    Sharapov, S. E.; Hellsten, T.; Kiptily, V. G.; Craciunescu, T.; Eriksson, J.; Fitzgerald, M.; Girardo, J.-B.; Goloborod'ko, V.; Hellesen, C.; Hjalmarsson, A.; Johnson, T.; Kazakov, Y.; Koskela, T.; Mantsinen, M.; Monakhov, I.; Nabais, F.; Nocente, M.; Perez von Thun, C.; Rimini, F.; Santala, M.; Schneider, M.; Tardocchi, M.; Tsalas, M.; Yavorskij, V.; Zoita, V.; Contributors, JET

    2016-11-01

    Dedicated fast ion D-D and D-3He fusion experiments were performed on JET with carbon wall (2008) and ITER-like wall (2014) for testing the upgraded neutron and energetic ion diagnostics of fusion products. Energy spectrum of D-D neutrons was the focus of the studies in pure deuterium plasmas. A significant broadening of the energy spectrum of neutrons born in D-D fast fusion was observed, and dependence of the maximum D and D-D neutron energies on plasma density was established. Diagnostics of charged products of aneutronic D-3He fusion reactions, 3.7 MeV alpha-particles similar to those in D-T fusion, and 14.6 MeV protons, were the focus of the studies in D-3He plasmas. Measurements of 16.4 MeV gamma-rays born in the weak secondary branch of D(3He, γ)5Li reaction were used for assessing D-3He fusion power. For achieving high yield of D-D and D-3He reactions at relatively low levels of input heating power, an acceleration of D beam up to the MeV energy range was used employing 3rd harmonic (f=3{{f}CD} ) ICRH technique. These results were compared to the techniques of D beam injection into D-3He mixture, and 3He-minority ICRH in D plasmas.

  20. Measurement of fast minority /sub 3/He/sup + +/ energy distribution during ICRF heating

    DOEpatents

    Post, D.E. Jr.; Grisham, L.R.; Medley, S.S.

    A method and means for measuring the fast /sub 3/He/sup + +/ distribution during /sub 3/He/sup + +/ minority Ion Cyclotron Resonance Frequency (ICRF) heating is disclosed. The present invention involves the use of 10 to 100 keV beams of neutral helium atoms to neutralize the fast /sub 3/He/sup + +/ ions in a heated plasma by double charge exchange (/sub 3/He/sup + +/ + /sub 4/He/sup 0/ ..-->.. /sub 3/He/sup 0/ + /sub 4/He/sup + +/). The neutralized fast /sub 3/He/sup 0/ atoms then escape from the hot plasma confined by a magnetic field and are detected by conventional neutral particle analyzing means. This technique permits the effectiveness of the coupling of the ion cyclotron waves to the /sub 3/He/sup + +/ minority ions to be accurately measured. The present invention is particularly adapted for use in evaluating the effectiveness of the intermediate coupling between the RF heating and the /sub 3/He/sup + +/ in an energetic toroidal plasma.

  1. On the connection between the 3HE-enrichment and spectral index of solar energetic particles

    NASA Technical Reports Server (NTRS)

    Kocharov, L. G.; Dvoryanchikov, Y. V.

    1985-01-01

    A model is presented which explains the observed tendency of events with large 3He/4He ratios to have steeper spectra. In this model preferential injection of 3He, acceleration by Alfven waves and Coulomb deceleration of ions are considered simultaneously. The observed tendency may be obtained as a result of competition between injection and acceleration processes.

  2. 4 He adsorption on a 3He-plated graphite surface

    NASA Astrophysics Data System (ADS)

    Kwon, Yongkyung; Ahn, Jeonghwan

    Path-integral Monte Carlo (PIMC) calculations have been performed for 4He atoms on top of the 3He first layer on graphite. For this we ignore Fermi statistics of solidified 3He adatoms while Bose statistics of 4He atoms are fully incorporated. We first find that the first 3He layer exhibits a 7/12 commensurate solid structure at the areal density of 0.111 Å-2, which turns out to be identical to the experimental value for its completion density. Additional adsorption of 4He atoms above the complete first 3He layer is found to sustain the underlying 3He commensurate structure and the second 4He layer is observed to display the 4/7 commensurate structure with respect to the first-layer commensurate 3He solid at the areal density of 0.0636 Å-2. Furthermore, it is found that the 4/7 commensurate structure of the second-layer 4He atoms can be formed above a mixture of the first-layer 3He and 4He atoms on graphite. These PIMC results suggest that the 4/7 commensurate structure of the second-layer 4He atoms on graphite, whose existence on top of the first 4He layer has long been in dispute, may be realized on a 3He-plated graphite surface. This could lead to a new approach to observe two-dimensional supersolidity in 4He on graphite.

  3. Surface Specific Heat of {sup 3}He and Andreev Bound States

    SciTech Connect

    Choi, H.; Davis, J. P.; Pollanen, J.; Halperin, W.P.

    2006-03-31

    High resolution measurements of the specific heat of liquid {sup 3}He in the presence of a silver surface have been performed at temperatures near the superfluid transition in the pressure range of 1-29 bar. The surface contribution to the heat capacity is identified with Andreev bound states of {sup 3}He quasiparticles that have a range of half a coherence length.

  4. Sterols of Pneumocystis carinii hominis Organisms Isolated from Human Lungs

    PubMed Central

    Kaneshiro, Edna S.; Amit, Zunika; Chandra, Jyotsna; Baughman, Robert P.; Contini, Carlo; Lundgren, Bettina

    1999-01-01

    The opportunistic pathogen Pneumocystis carinii causes pneumonia (P. carinii pneumonia, or PCP) in immunocompromised individuals such as AIDS patients. Rat-derived P. carinii carinii organisms have distinct sterols which are not synthesized by mammals and not found in other microbes infecting mammalian lungs. The dominant sterol present in the organism is cholesterol (which is believed to be scavenged from the host), but other sterols in P. carinii carinii have an alkyl group at C-24 of the sterol side chain (C28 and C29 24-alkylsterols) and a double bond at C-7 of the nucleus. Recently, pneumocysterol (C32), which is essentially lanosterol with a C-24 ethylidene group, was detected in lipids extracted from a formalin-fixed human P. carinii-infected lung, and its structures were elucidated by gas-liquid chromatography, mass spectrometry, and nuclear magnetic resonance spectrometry in conjunction with analyses of chemically synthesized authentic standards. The sterol composition of isolated P. carinii hominis organisms has yet to be reported. If P. carinii from animal models is to be used for identifying potential drug targets and for developing chemotherapeutic approaches to clear human infections, it is important to determine whether the 24-alkylsterols of organisms found in rats are also present in organisms in humans. In the present study, sterol analyses of P. carinii hominis organisms isolated from cryopreserved human P. carinii-infected lungs and from bronchoalveolar lavage fluid were performed. Several of the same distinct sterols (e.g., fungisterol and methylcholest-7-ene-3β-ol) previously identified in P. carinii carinii were also present in organisms isolated from human specimens. Pneumocysterol was detected in only some of the samples. PMID:10548595

  5. Autoradiographic localization of beta-adrenoceptors in asthmatic human lung

    SciTech Connect

    Spina, D.; Rigby, P.J.; Paterson, J.W.; Goldie, R.G. )

    1989-11-01

    The autoradiographic distribution and density of beta-adrenoceptors in human non-diseased and asthmatic bronchi were investigated using (125I)iodocyanopindolol (I-CYP). Analysis of the effects of the beta-adrenoceptor antagonists on I-CYP binding demonstrated that betaxolol (20 nM, beta 1-selective) had no significant effect on specific grain density in either nonasthmatic or asthmatic human bronchus, whereas ICI-118551 (20 nM, beta 2-selective) inhibited I-CYP binding by 85 +/- 9% and 89 +/- 3%, respectively. Thus, homogeneous populations of beta 2-adrenoceptors existed in bronchi from both sources. Large populations of beta-adrenoceptors were localized to the bronchial epithelium, submucosal glands, and airway smooth muscle. Asthmatic bronchial tissue featured epithelial damage with exfoliated cells associated with luminal mucus plugs. A thickened basement membrane and airway smooth muscle hyperplasia were also evident. High levels of specific I-CYP binding were also detected over asthmatic bronchial smooth muscle, as assessed by autoradiography and quantitation of specific grain densities. Isoproterenol and fenoterol were 10- and 13-fold less potent, respectively, in bronchi from asthmatic lung than in those from nonasthmatic lung. However, this attenuated responsiveness to beta-adrenoceptor agonists was not caused by reduced beta-adrenoceptor density in asthmatic airways. A defect may exist in the coupling between beta-adrenoceptors and postreceptor mechanisms in severely asthmatic lung.

  6. Characterization of muscarinic cholinergic receptor subtypes in human peripheral lung

    SciTech Connect

    Bloom, J.W.; Halonen, M.; Yamamura, H.I.

    1988-02-01

    The authors have characterized the muscarinic cholinergic receptor subtypes in human peripheral lung membranes using the selective muscarinic antagonist (/sup 3/H)pirenzepine ((/sup 3/H)PZ) and the classical muscarinic antagonist (/sup 3/H)(-)-quinuclidinyl benzilate. High-affinity binding with pharmacologic specificity was demonstrated for both radioligands. The high affinity Kd for (/sup 3/H)PZ binding determined from saturation isotherms was 5.6 nM, and the Kd for (/sup 3/H)(-)-quinuclidinyl benzilate binding was 14.3 pM. Approximately 62% of the total muscarinic binding sites in human peripheral lung bind (/sup 3/H)PZ with high affinity. There was no significant effect of the guanine nucleotide, guanyl-5'-yl imidodiphosphate, on the inhibition of (/sup 3/H)(-)-quinyclidinyl benzilate binding by the muscarinic agonist carbachol in peripheral lung membranes. If the muscarinic receptor with high affinity for PZ has an important role in bronchoconstriction, its characterization could result in the development of more selective bronchodilators.

  7. A numerical study of gas transport in human lung models

    NASA Astrophysics Data System (ADS)

    Lin, Ching-Long; Hoffman, Eric A.

    2005-04-01

    Stable Xenon (Xe) gas has been used as an imaging agent for decades in its radioactive form, is chemically inert, and has been used as a ventilation tracer in its non radioactive form during computerized tomography (CT) imaging. Magnetic resonance imaging (MRI) using hyperpolarized Helium (He) gas and Xe has also emerged as a powerful tool to study regional lung structure and function. However, the present state of knowledge regarding intra-bronchial Xe and He transport properties is incomplete. As the use of these gases rapidly advances, it has become critically important to understand the nature of their transport properties and to, in the process, better understand the role of gas density in general in determining regional distribution of respiratory gases. In this paper, we applied the custom developed characteristic-Galerkin finite element method, which solves the three-dimensional (3D) incompressible variable-density Navier-Stokes equations, to study the transport of Xe and He in the CT-based human lung geometries, especially emulating the washin and washout processes. The realistic lung geometries are segmented and reconstructed from CT images as part of an effort to build a normative atlas (NIH HL-064368) documenting airway geometry over 4 decades of age in healthy and disease-state adult humans. The simulation results show that the gas transport process depends on the gas density and the body posture. The implications of these results on the difference between washin and washout time constants are discussed.

  8. Nuclear georeactor origin of oceanic basalt 3He/4He, evidence, and implications

    PubMed Central

    Herndon, J. Marvin

    2003-01-01

    Nuclear georeactor numerical simulation results yield substantial 3He and 4He production and 3He/4He ratios relative to air (RA) that encompass the entire 2-SD (2σ) confidence level range of tabulated measured 3He/4He ratios of basalts from along the global spreading ridge system. Georeactor-produced 3He/4He ratios are related to the extent of actinide fuel consumption at time of production and are high near the end of the georeactor lifetime. Georeactor numerical simulation results and the observed high 3He/4He ratios measured in Icelandic and Hawaiian oceanic basalts indicate that the demise of the georeactor is approaching. Within the present level of uncertainty, one cannot say precisely when georeactor demise will occur, whether in the next century, in a million years, or in a billion years from now. PMID:12615991

  9. Variational calculations for ground state properties of liquid 3He injected in a carbon nanotube

    NASA Astrophysics Data System (ADS)

    Bordbar, G. H.; Rastkhadiv, M. A.

    2017-09-01

    Liquid 3He injected in a carbon nanotube is of high interests due to different behavior of the liquid helium in the quasi-one-dimensional systems. In this work, a variational approach has been performed to calculate some thermodynamic properties of this quantum system. In order to do so, a single-walled carbon nanotube containing liquid 3He is considered, applying the Lennard-Jones and Stan-Cole potentials for 3He-3He and 3He-C interactions, respectively. Finally the total energy, equation of state and incompressibility of the system have been calculated. Our calculations show the high values for the incompressibility at high densities, especially for high radii.

  10. Development of a polarized 3He neutron spin filter for POLANO at J-PARC

    NASA Astrophysics Data System (ADS)

    Ino, T.; Ohkawara, M.; Ohoyama, K.; Yokoo, T.; Itoh, S.; Nambu, Y.; Fujita, M.; Kira, H.; Hayashida, H.; Hiroi, K.; Sakai, K.; Oku, T.; Kakurai, K.

    2017-06-01

    We have developed a polarized 3He neutron spin filter (NSF) for a new polarized neutron spectrometer, POLANO, at the Japan Proton Accelerator Research Complex (J-PARC). POLANO aims to utilize high energy neutrons polarized by a 3He NSF and spin analyzed by an array of magnetic supermirrors for inelastic neutron scattering. The 3He gas is continuously polarized in-situ by spin-exchange optical pumping to provide a highly and stably polarized neutron beam. The POLANO 3He NSF is designed to polarize neutrons with energies as high as 200 meV and fit in a restricted space. It is equipped with adiabatic fast passage NMR that enables one to flip the 3He spins, and consequently, the neutron spins.

  11. Nuclear georeactor origin of oceanic basalt 3He/4He, evidence, and implications.

    PubMed

    Herndon, J Marvin

    2003-03-18

    Nuclear georeactor numerical simulation results yield substantial (3)He and (4)He production and (3)He(4)He ratios relative to air (R(A)) that encompass the entire 2-SD (2sigma) confidence level range of tabulated measured (3)He(4)He ratios of basalts from along the global spreading ridge system. Georeactor-produced (3)He(4)He ratios are related to the extent of actinide fuel consumption at time of production and are high near the end of the georeactor lifetime. Georeactor numerical simulation results and the observed high (3)He(4)He ratios measured in Icelandic and Hawaiian oceanic basalts indicate that the demise of the georeactor is approaching. Within the present level of uncertainty, one cannot say precisely when georeactor demise will occur, whether in the next century, in a million years, or in a billion years from now.

  12. Application of Sol-Gel Technology to High Pressure Polarized 3HE Nuclear Targets

    NASA Astrophysics Data System (ADS)

    Tobias, W. A.; Cates, G. D.; Chaput, J.; Deur, A.; Rohrbaugh, S.; Singh, J.

    2003-01-01

    High-purity sol-gel solutions have been developed to coat the interior surface of glass vessels used for polarizing 3He by spin-exchange optical pumping. Such cells have been shown to exhibit 3He longitudinal lifetimes T1 in excess of 350 hours1. The sol-gel technique was designed to minimize spin-relaxation due to wall collisions so that only dipole-dipole interactions between colliding 3He atoms dominate in the relaxation process. Until now, sol-gel technology had not been applied to high pressure 3He gas targets used in nuclear scattering experiments. A description of the sol-gel technique and recent developments on its integration into the production of 3He targets will be presented.

  13. Rewiring of human lung cell lineage and mitotic networks in lung adenocarcinomas

    PubMed Central

    Kim, Il-Jin; Quigley, David; To, Minh D.; Pham, Patrick; Lin, Kevin; Jo, Brian; Jen, Kuang-Yu; Raz, Dan; Kim, Jae; Mao, Jian-Hua; Jablons, David; Balmain, Allan

    2015-01-01

    Analysis of gene expression patterns in normal tissues and their perturbations in tumors can help to identify the functional roles of oncogenes or tumor suppressors and identify potential new therapeutic targets. Here, gene expression correlation networks were derived from 92 normal human lung samples and patient-matched adenocarcinomas. The networks from normal lung show that NKX2-1 is linked to the alveolar type 2 lineage, and identify PEBP4 as a novel marker expressed in alveolar type 2 cells. Differential correlation analysis shows that the NKX2-1 network in tumors includes pathways associated with glutamate metabolism, and identifies Vaccinia-related kinase (VRK1) as a potential drug target in a tumor-specific mitotic network. We show that VRK1 inhibition cooperates with inhibition of PARP signaling to inhibit growth of lung tumor cells. Targeting of genes that are recruited into tumor mitotic networks may provide a wider therapeutic window than that seen by inhibition of known mitotic genes. PMID:23591868

  14. Sex-specific Differences in Hyperoxic Lung Injury in Mice: Implications for Acute and Chronic Lung Disease in Humans

    PubMed Central

    Lingappan, Krithika; Jiang, Weiwu; Wang, Lihua; Couroucli, Xanthi I.; Barrios, Roberto; Moorthy, Bhagavatula

    2014-01-01

    Sex-specific differences in pulmonary morbidity in humans are well documented. Hyperoxia contributes to lung injury in experimental animals and humans. The mechanisms responsible for sex differences in the susceptibility towards hyperoxic lung injury remain largely unknown. In this investigation, we tested the hypothesis that mice will display sex-specific differences in hyperoxic lung injury. Eight week-old male and female mice (C57BL/6J) were exposed to 72 h of hyperoxia (FiO2>0.95). After exposure to hyperoxia, lung injury, levels of 8-iso-prostaglandin F2 alpha (8-iso-PGF 2α) (LC-MS/MS), apoptosis (TUNEL) and inflammatory markers (suspension bead array) were determined. CytochromeP450 (CYP)1A expression in the lung was assessed using immunohistochemistry and western blotting. After exposure to hyperoxia, males showed greater lung injury, neutrophil infiltration and apoptosis, compared to air-breathing controls than females. Pulmonary 8-iso-PGF 2α levels were higher in males than females after hyperoxia exposure. Sexually dimorphic increases in levels of IL-6 (F>M) and VEGF (M>F) in the lungs were also observed. CYP1A1 expression in the lung was higher in female mice compared to males under hyperoxic conditions. Overall, our results support the hypothesis that male mice are more susceptible than females to hyperoxic lung injury and that differences in inflammatory and oxidative stress markers contribute to these sex-specific dimorphic effects. In conclusion, this paper describes the establishment of an animal model that shows sex differences in hyperoxic lung injury in a temporal manner and thus has important implications for lung diseases mediated by hyperoxia in humans. PMID:23792423

  15. Three-dimensional scaffolds of acellular human and porcine lungs for high throughput studies of lung disease and regeneration

    PubMed Central

    Wagner, Darcy E.; Bonenfant, Nicholas R.; Sokocevic, Dino; DeSarno, Michael; Borg, Zachary; Parsons, Charles; Brooks, Elice M.; Platz, Joseph; Khalpey, Zain; Hoganson, David M.; Deng, Bin; Lam, Ying Wai; Oldinski, Rachael A.; Ashikaga, Takamaru; Weiss, Daniel J.

    2014-01-01

    Acellular scaffolds from complex whole organs such as lung are being increasingly studied for ex vivo organ generation and for in vitro studies of cell-extracellular matrix interactions. We have established effective methods for efficient de- and recellularization of large animal and human lungs including techniques which allow multiple small segments (∼1–3cm3) to be excised that retain 3-dimensional lung structure. Coupled with the use of a synthetic pleural coating, cells can be selectively physiologically inoculated via preserved vascular and airway conduits. Inoculated segments can be further sliced for high throughput studies. Further, we demonstrate thermography as a powerful noninvasive technique for monitoring perfusion decellularization and for evaluating preservation of vascular and airway networks following human and porcine lung decellularization. Collectively, these techniques are a significant step forward as they allow high throughput in vitro studies from a single lung or lobe in a more biologically relevant, three-dimensional acellular scaffold. PMID:24411675

  16. 3-D segmentation of human sternum in lung MDCT images.

    PubMed

    Pazokifard, Banafsheh; Sowmya, Arcot

    2013-01-01

    A fully automatic novel algorithm is presented for accurate 3-D segmentation of the human sternum in lung multi detector computed tomography (MDCT) images. The segmentation result is refined by employing active contours to remove calcified costal cartilage that is attached to the sternum. For each dataset, costal notches (sternocostal joints) are localized in 3-D by using a sternum mask and positions of the costal notches on it as reference. The proposed algorithm for sternum segmentation was tested on 16 complete lung MDCT datasets and comparison of the segmentation results to the reference delineation provided by a radiologist, shows high sensitivity (92.49%) and specificity (99.51%) and small mean distance (dmean=1.07 mm). Total average of the Euclidean distance error for costal notches positioning in 3-D is 4.2 mm.

  17. [Air distribution in the bronchial tree of human lungs].

    PubMed

    Lai, Wei; Tan, Xiaoping; Pei, Juemin

    2004-04-01

    A three-element model of lumped parameter based on the statistic data of Weible's symmetric model and on the reference anatomic figures of the bronchial tree of the lungs has been proposed using the fluid network theory. It was assumed that the upper five or seven generations of the twenty-four generations of the respiratory airway are asymmetric, while the rest are symmetric. GEAR method was used to solve the ordinary differential equations. The pressure and flow rate distributions in different positions of the lungs during normal respiration and partial bronchial obstruction were compared, respectively. This model has great significance in finding out the air distribution in the human bronchial tree under various physiological and pathological conditions.

  18. T(T,4He)2n and 3He(3He,4He)2p Reactions and the Energy Dependence of Their Mechanisms

    NASA Astrophysics Data System (ADS)

    Bacher, Andrew; McNabb, Dennis; Brune, Carl; Sayre, Dan; Hale, Gerry; Frenje, Johan; Gatu Johnson, Maria

    2015-10-01

    We have studied the T(T,alpha)2n reaction because it is the charge symmetric analog to the 3He(3He,alpha)2p reaction which completes the most direct mode of the p-p chain in stellar interiors. These reactions lead to three-body final states whose energy spectrum shapes are dominated by the strong nucleon-alpha interaction and the weaker nucleon-nucleon interaction. These experiments were done at OMEGA at the University of Rochester and at the NIF at Lawrence Livermore Lab. We will focus on two features: (1) the excitation energy dependence of the reaction mechanism and (2) the center-of-mass energy dependence of the reaction mechanism. At stellar energies (OMEGA and the NIF) we find that the shape of the neutron spectrum peaks in the middle. The n-alpha 1/2-excited state is about two times stronger than the n-alpha 3/2-ground state. For the 3He+3He reaction (at CalTech), the proton spectrum peaks at the high end. The p-alpha 3/2-state is about two times stronger than the 1/2-state. This difference in the spectrum shape is explained by theoretical models which include the interference between the two identical fermions in the final state. At CalTech we have angular distributions of the 3He+3He reaction from 2 MeV to 18 MeV. We see the p-wave strength increasing.

  19. In vivo quantification of human lung dose response relationship

    NASA Astrophysics Data System (ADS)

    O'Dell, Walter; Wang, Peng; Liu, Haisong; Fuller, David; Schell, Michael C.; Okunieff, Paul

    2007-03-01

    Purpose: To implement a new non-invasive in-vivo assay to compute the dose-response relationship following radiation-induced injury to normal lung tissue, using computed tomography (CT) scans of the chest. Methods and Materials: Follow-up volumetric CT scans were acquired in patients with metastatic tumors to the lung treated using stereotactic radiation therapy. The images reveal a focal region of fibrosis corresponding to the high-dose region and no observable long-term damage in distant sites. For each pixel in the follow-up image the treatment dose and the change in apparent tissue density was compiled. For each of 12 pre-selected dose levels the average pixel tissue density change was computed and fit to a two-parameter dose-response model. The sensitivity of the resulting fits to registration error was also quantified. Results: Complete in vivo dose-response relationships in human normal lung tissue were computed. Increasing radiation sensitivity was found with larger treatment volume. Radiation sensitivity increased also over time up to 12 months, but decreased at later time points. The time-course of dose response correlated with the time-course of levels of circulating IL-1α, TGFβ and MCP-1. The method was found to be robust to registration errors up to 3 mm. Conclusions: This approach for the first time enables the quantification of the full range dose response relationship in human subjects. The method may be used to assess quantitatively the efficacy of various agents thought to illicit radiation protection to the lung.

  20. Interpretation of the Processes 3He(e,e'p)2H and 3He(e,e'p)(pn) at High Missing Momenta

    NASA Astrophysics Data System (ADS)

    Ciofi Degli Atti, C.; Kaptari, L. P.

    2005-07-01

    Using realistic three-body wave functions corresponding to the AV18 interaction, it is shown that the effects of the final state interaction in the exclusive processes 3He(e,e'p)2H and 3He(e,e'p)(pn), can be successfully treated in terms of a generalized eikonal approximation based upon the direct calculation of the Feynman diagrams describing the rescattering of the struck nucleon. The relevant role played by the double rescattering contribution at high values of the missing momentum is illustrated.

  1. Interpretation of the processes 3He(e,e'p)2H and 3He(e,e'p)(pn) at high missing momenta.

    PubMed

    Ciofi degli Atti, C; Kaptari, L P

    2005-07-29

    Using realistic three-body wave functions corresponding to the AV18 interaction, it is shown that the effects of the final state interaction in the exclusive processes 3He(e,e'p)2H and 3He(e,e'p)(pn), can be successfully treated in terms of a generalized eikonal approximation based upon the direct calculation of the Feynman diagrams describing the rescattering of the struck nucleon. The relevant role played by the double rescattering contribution at high values of the missing momentum is illustrated.

  2. T(T,2n)4He and 3He(3He,2p)4He: The Reaction Mechanism from Solar Energies to 10 MeV

    NASA Astrophysics Data System (ADS)

    Bacher, A. D.; Brune, C. R.; Sayre, D. B.; Hale, G. M.; Frenje, J. A.; Gatu Johnson, M.

    2016-03-01

    We have studied the energy dependence of the reaction mechanism of the T(t,2n)4He reaction at stellar energies and of its charge symmetric analog reaction 3He(3He,2p)4He at energies up 10 MeV. We find that the reaction mechanism changes dramatically over this energy range in part due to the interference of the two identical fermions in the three-body final state. This contribution is dedicated to the memory of Tom Tombrello, my Ph.D. advisor at Cal Tech, who died in 2014.

  3. Search for anomalous deuterons in the reaction /sup 3/He+C. -->. d+X at p3He = 10. 8 GeV/c

    SciTech Connect

    Ableev, V.G.; Vorob'ev, G.G.; Gasparyan, A.P.; Grigalashvili, N.S.; Dzhmukhadze, S.V.; Zaporozhets, S.A.; Nomofilov, A.A.; Piskunov, N.M.; Sitnik, I.M.; Strokovskii, E.A.

    1985-07-01

    The Alpha apparatus in a beam of 10.78-GeV/c /sup 3/He nuclei has been used to search for anomalous deuterons (demons) in the reaction /sup 3/He+C ..-->.. d+X. The deuteron yield was measured at angles theta< or approx. =20 mrad as a function of the target thickness, which varied from 0.6 to 70 cm. The data obtained exclude the production of demons in this reaction over a wide range of expected values of the cross sections for their production and interaction with matter. The technique used can be applied also to beams of relativistic nuclei in experiments to search for anomalons.

  4. Plate Tectonic Cycling and Whole Mantle Convection Modulate Earth's 3He/22Ne Ratio

    NASA Astrophysics Data System (ADS)

    Dygert, N. J.; Jackson, C.; Hesse, M. A.; Tremblay, M. M.; Shuster, D. L.; Gu, J.

    2016-12-01

    3He and 22Ne are not produced in the mantle or fractionated by partial melting, and neither isotope is recycled back into the mantle by subduction of oceanic basalt or sediment. Thus, it is a surprise that large 3He/22Ne variations exist within the mantle and that the mantle has a net elevated 3He/22Ne ratio compared to volatile-rich planetary precursor materials. Depleted subcontinental lithospheric mantle and mid-ocean ridge basalt (MORB) mantle have distinctly higher 3He/22Ne compared to ocean island basalt (OIB) sources ( 4-12.5 vs. 2.5-4.5, respectively) [1,2]. The low 3He/22Ne of OIBs approaches chondritic ( 1) and solar nebula values ( 1.5). The high 3He/22Ne of the MORB mantle is not similar to solar sources or any known family of meteorites, requiring a mechanism for fractionating He from Ne in the mantle and suggesting isolation of distinct mantle reservoirs throughout geologic time. We model the formation of a MORB source with elevated and variable 3He/22Ne though diffusive exchange between dunite channel-hosted basaltic liquids and harzburgite wallrock beneath mid-ocean ridges. Over timescales relevant to mantle upwelling beneath spreading centers, He may diffuse tens to hundreds of meters into wallrock while Ne is relatively immobile, producing a regassed, depleted mantle lithosphere with elevated 3He/22Ne. Subduction of high 3He/22Ne mantle would generate a MORB source with high 3He/22Ne. Regassed, high 3He/22Ne mantle lithosphere has He concentrations 2-3 orders of magnitude lower than undegassed mantle. To preserve the large volumes of high 3He/22Ne mantle required by the MORB source, mixing between subducted and undegassed mantle reservoirs must have been limited throughout geologic time. Using the new 3He/22Ne constraints, we ran a model similar to [3] to quantify mantle mixing timescales, finding they are on the order of Gyr assuming physically reasonable seafloor spreading rates, and that Earth's convecting mantle has lost >99% of its primordial

  5. Decellularization of human and porcine lung tissues for pulmonary tissue engineering.

    PubMed

    O'Neill, John D; Anfang, Rachel; Anandappa, Annabelle; Costa, Joseph; Javidfar, Jeffrey; Wobma, Holly M; Singh, Gopal; Freytes, Donald O; Bacchetta, Matthew D; Sonett, Joshua R; Vunjak-Novakovic, Gordana

    2013-09-01

    The only definitive treatment for end-stage organ failure is orthotopic transplantation. Lung extracellular matrix (LECM) holds great potential as a scaffold for lung tissue engineering because it retains the complex architecture, biomechanics, and topologic specificity of the lung. Decellularization of human lungs rejected from transplantation could provide "ideal" biologic scaffolds for lung tissue engineering, but the availability of such lungs remains limited. The present study was designed to determine whether porcine lung could serve as a suitable substitute for human lung to study tissue engineering therapies. Human and porcine lungs were procured, sliced into sheets, and decellularized by three different methods. Compositional, ultrastructural, and biomechanical changes to the LECM were characterized. The suitability of LECM for cellular repopulation was evaluated by assessing the viability, growth, and metabolic activity of human lung fibroblasts, human small airway epithelial cells, and human adipose-derived mesenchymal stem cells over a period of 7 days. Decellularization with 3-[(3-Cholamidopropyl)dimethylammonio]-1-propanesulfonate (CHAPS) showed the best maintenance of both human and porcine LECM, with similar retention of LECM proteins except for elastin. Human and porcine LECM supported the cultivation of pulmonary cells in a similar way, except that the human LECM was stiffer and resulted in higher metabolic activity of the cells than porcine LECM. Porcine lungs can be decellularized with CHAPS to produce LECM scaffolds with properties resembling those of human lungs, for pulmonary tissue engineering. We propose that porcine LECM can be an excellent screening platform for the envisioned human tissue engineering applications of decellularized lungs. Copyright © 2013 The Society of Thoracic Surgeons. Published by Elsevier Inc. All rights reserved.

  6. Third sound and stability of 3He-4He mixture films

    SciTech Connect

    Anderson, R. H.; Krotscheck, E.; Miller, M. D.

    2006-09-07

    We study third sound and the interaction between 3He adatoms in two thin 3He-4He mixture films from a first-principles, microscopic theory. Utilizing the variational, hypernetted-chain Euler-Lagrange (HNC-EL) theory as applied to inhomogeneous boson systems, we calculate chemical potentials for both the 4He superfluid film and the physisorbed 3He. Numerical density derivatives of the chemical potentials lead to the sought-after third sound speeds that clearly reflect a layered structure of at least seven oscillations. In this paper, we report third sound on model substrates: Nuclepore, and sodium. We find that the effect of the 3He depends sensitively on the particular 4He film coverage. Our most important result is that, with the addition of 3He, the third sound speed can either increase or decrease. In fact, in some regimes, the added 3He destabilizes the film and can drive ''layering transitions'', leading to fairly complicated geometric structures of the film in which the outermost layer is predicted to consist of phase-separated regions of 3He and 4He.

  7. The cosmological density of baryons from observations of 3He+ in the Milky Way.

    PubMed

    Bania, T M; Rood, Robert T; Balser, Dana S

    2002-01-03

    Primordial nucleosynthesis after the Big Bang can be constrained by the abundances of the light elements and isotopes 2H, 3He, 4He and 7Li (ref. 1). The standard theory of stellar evolution predicts that 3He is also produced by solar-type stars, so its abundance is of interest not only for cosmology, but also for understanding stellar evolution and the chemical evolution of the Galaxy. The 3He abundance in star-forming (H II) regions agrees with the present value for the local interstellar medium, but seems to be incompatible with the stellar production rates inferred from observations of planetary nebulae, which provide a direct test of stellar evolution theory. Here we develop our earlier observations, which, when combined with recent theoretical developments in our understanding of light-element synthesis and destruction in stars, allow us to determine an upper limit for the primordial abundance of 3He relative to hydrogen: 3He/H = (1.1 +/- 0.2) x 10(-5). The primordial density of all baryons determined from the 3He data is in excellent agreement with the densities calculated from other cosmological probes. The previous conflict is resolved because most solar-mass stars do not produce enough 3He to enrich the interstellar medium significantly.

  8. Enhanced IR hollow cathode laser in a 3He Ne gas mixture

    NASA Astrophysics Data System (ADS)

    Stefanova, M. S.; Pramatarov, P. M.; Karelin, A. V.

    2005-09-01

    An experimental and theoretical study on 3He-Ne and 4He-Ne helical hollow cathode lasers is presented. Enhanced laser operation on the near IR NeI lines is observed when the natural isotope 4He is substituted by the lighter isotope 3He. A four-fold increase in the laser output power and a three-fold increase in the laser gain for the strongest NeI 1.1523 µm line is measured in the 3He-Ne gas mixture compared to the 4He-Ne gas mixture. On the basis of the theoretical analysis done by means of a non-stationary kinetic model for the negative glow plasma of 3He-Ne and 4He-Ne hollow cathode lasers, a study on the changes in the particle kinetics is carried out and an explanation of the experimental results is proposed. In the 3He-Ne mixture the electron temperature is lower than in the 4He-Ne mixture, while the gas temperature is higher. As a result the helium triplet metastable density and the rate constant for excitation transfer to neon atoms are higher in the 3He-Ne mixture. The lower laser level de-excitation due to intra-multiplet mixing of 2p1-10levels by 3He atoms is more efficient.

  9. Gas cells for 3He hyperpolarized via spin-exchange optical pumping

    NASA Astrophysics Data System (ADS)

    Kim, W.; Stepanyan, S. S.; Kim, A.; Tan, J. A.; Woo, S.

    2016-01-01

    We present a device for the production of hyperpolarized 3He, which is widely used in spinrelated nuclear physics research. Spin-exchange optical pumping (SEOP) is employed to polarize 3He enclosed in a circular borosilicate glass cell suitable not only for the production of polarized gas but also for its storage. The portable glass cell can, thus, be transported to any other research facility. The glass cell can be refilled several times. Special attention is given to the preparation and the filling of the cell to minimize the impurities on its walls and in the gas. We employ glass tubes with shorter lengths and larger diameters in the gas-filling system to achieve the improvement in the air flow necessary to obtain purer polarized 3He samples. The cell is prepared, and after it has been filled with rubidium (Rb) and 3He-N2 mixture, it is sealed under high vacuum conditions. The cell containing the mixture is exposed to circularly-polarized laser light with a wavelength of 795 nm at temperatures of 180 - 220 °C for SEOP. The polarization of 3He is measured via nuclear magnetic resonance (NMR). We obtained 40% polarized 3He in less than 15 hours and 50% in about 25 hours. The longitudinal relaxation time T 1 of the polarized 3He we measured was about 58 hours.

  10. AFP flipper devices: Polarized 3He spin flipper and shorter wavelength neutron flipper

    NASA Astrophysics Data System (ADS)

    Babcock, E.; Petoukhov, A.; Chastagnier, J.; Jullien, D.; Lelièvre-Berna, E.; Andersen, K. H.; Georgii, R.; Masalovich, S.; Boag, S.; Frost, C. D.; Parnell, S. R.

    2007-07-01

    We describe the development of a polarized neutron device that combines a 3He neutron spin filter and a neutron spin flipper using adiabatic fast passage (AFP), to adiabatically reverse the 3He polarization and thus the neutron polarization with near perfect symmetry. A typical AFP sequence takes place in 2.5-7.5 ms, with the time for the 3He transition from P to -P much less, thus the neutron polarization is nearly perfectly reversed very quickly with only a 2×10-5 loss in 3He polarization per flip. We believe this device, the 3He “flipperizer” can become a standard option wherever a 3He spin filter is already in use. Our first on beam test was performed on MIRA at the new FRM-2 reactor in Garching using polarized 3He from HELIOS. We also briefly describe tests of a new neutron flipper based on AFP. This broad band neutron RF flipper was shown to create neutron flipping efficiencies of >99% at a neutron wavelength of 0.4 Å. Neutron tests were performed on D3 (ILL) and on ROTAX (ISIS).

  11. Recent advances in polarized 3 He based neutron spin filter development

    NASA Astrophysics Data System (ADS)

    Chen, Wangchun; Gentile, Thomas; Erwin, Ross; Watson, Shannon; Krycka, Kathryn; Ye, Qiang; NCNR NIST Team; University of Maryland Team

    2015-04-01

    Polarized 3 He neutron spin filters (NSFs) are based on the strong spin-dependence of the neutron absorption cross section by 3 He. NSFs can polarize large area, widely divergent, and broadband neutron beams effectively and allow for combining a neutron polarizer and a spin flipper into a single polarizing device. The last capability utilizes 3 He spin inversion based on the adiabatic fast passage (AFP) nuclear magnetic resonance technique. Polarized 3 He NSFs are significantly expanding the polarized neutron measurement capabilities at the NIST Center for Neutron Research (NCNR). Here we present an overview of 3 He NSF applications to small-angle neutron scattering, thermal triple axis spectrometry, and wide-angle polarization analysis. We discuss a recent upgrade of our spin-exchange optical pumping (SEOP) systems that utilize chirped volume holographic gratings for spectral narrowing. The new capability allows us to polarize rubidium/potassium hybrid SEOP cells over a liter in volume within a day, with 3 He polarizations up to 88%, Finally we discuss how we can achieve nearly lossless 3 He polarization inversion with AFP.

  12. Cosmogenic 3He in terrestrial rocks: The summit lavas of Maui

    PubMed Central

    Craig, H.; Poreda, R. J.

    1986-01-01

    We have identified terrestrial cosmic rayproduced 3He in three lava flows on the crest of Haleakala Volcano on Maui, 3 km above sea level, and ≈0.5 million years old. Although these lavas, like all oceanic basalts, contain primordial 3He from the mantle, the “cosmogenic” component (3HeC) can be identified unambiguously because it is extractable only by high-temperature vacuum fusion. In contrast, a large fraction of the mantle helium resides in fluid inclusions and can be extracted by vacuum crushing, leaving a residual component with 3He/4He ratios as high as 75× those in the atmosphere, which can be liberated by melting the crushed grains. Cosmogenic 3He is present in both olivines and clinopyroxenes at 0.8-1.2 × 10-12 ml(STP)/g and constitutes 75% ± 5% of the total 3He present. The observed 3HeC levels require a cosmic ray exposure age of only some 64,000 years, much less than the actual age of the lavas, if there is no erosion. Using a model that includes effects of uplift or submergence as well as erosion, we calculate an apparent “erosion rate” of the order of 8.5 m/106 years for the western rim of the summit crater, as an example of the application of measurements of cosmogenic rare gases to terrestrial geological problems. PMID:16593671

  13. Anomalous yield reduction in direct-drive DT implosions due to 3He addition

    SciTech Connect

    Herrmann, Hans W; Langenbrunner, James R; Mack, Joseph M; Cooley, James H; Wilson, Douglas C; Evans, Scott C; Sedillo, Tom J; Kyrala, George A; Caldwell, Stephen E; Young, Carlton A; Nobile, Arthur; Wermer, Joseph R; Paglieri, Stephen N; Mcevoy, Aaron M; Kim, Yong Ho; Batha, Steven H; Horsfield, Colin J; Drew, Dave; Garbett, Warren; Rubery, Michael; Glebov, Vladimir Yu; Roberts, Samuel; Frenje, Johan A

    2008-01-01

    Glass capsules were imploded in direct drive on the OMEGA laser [T. R. Boehly et aI., Opt. Commun. 133, 495, 1997] to look for anomalous degradation in deuterium/tritium (DT) yield (i.e., beyond what is predicted) and changes in reaction history with {sup 3}He addition. Such anomalies have previously been reported for D/{sup 3}He plasmas, but had not yet been investigated for DT/{sup 3}He. Anomalies such as these provide fertile ground for furthering our physics understanding of ICF implosions and capsule performance. A relatively short laser pulse (600 ps) was used to provide some degree of temporal separation between shock and compression yield components for analysis. Anomalous degradation in the compression component of yield was observed, consistent with the 'factor of two' degradation previously reported by MIT at a 50% {sup 3}He atom fraction in D{sub 2} using plastic capsules [Rygg et aI., Phys. Plasmas 13, 052702 (2006)]. However, clean calculations (i.e., no fuel-shell mixing) predict the shock component of yield quite well, contrary to the result reported by MIT, but consistent with LANL results in D{sub 2}/{sup 3}He [Wilson, et aI., lml Phys: Conf Series 112, 022015 (2008)]. X-ray imaging suggests less-than-predicted compression ofcapsules containing {sup 3}He. Leading candidate explanations are poorly understood Equation-of-State (EOS) for gas mixtures, and unanticipated particle pressure variation with increasing {sup 3}He addition.

  14. The HSP90 Inhibitor Ganetespib Radiosensitizes Human Lung Adenocarcinoma Cells

    PubMed Central

    Gomez-Casal, Roberto; Bhattacharya, Chitralekha; Epperly, Michael W.; Basse, Per H.; Wang, Hong; Wang, Xinhui; Proia, David A.; Greenberger, Joel S.; Socinski, Mark A.; Levina, Vera

    2015-01-01

    The molecular chaperone HSP90 is involved in stabilization and function of multiple client proteins, many of which represent important oncogenic drivers in NSCLC. Utilization of HSP90 inhibitors as radiosensitizing agents is a promising approach. The antitumor activity of ganetespib, HSP90 inhibitor, was evaluated in human lung adenocarcinoma (AC) cells for its ability to potentiate the effects of IR treatment in both in vitro and in vivo. The cytotoxic effects of ganetespib included; G2/M cell cycle arrest, inhibition of DNA repair, apoptosis induction, and promotion of senescence. All of these antitumor effects were both concentration- and time-dependent. Both pretreatment and post-radiation treatment with ganetespib at low nanomolar concentrations induced radiosensitization in lung AC cells in vitro. Ganetespib may impart radiosensitization through multiple mechanisms: such as down regulation of the PI3K/Akt pathway; diminished DNA repair capacity and promotion of cellular senescence. In vivo, ganetespib reduced growth of T2821 tumor xenografts in mice and sensitized tumors to IR. Tumor irradiation led to dramatic upregulation of β-catenin expression in tumor tissues, an effect that was mitigated in T2821 xenografts when ganetespib was combined with IR treatments. These data highlight the promise of combining ganetespib with IR therapies in the treatment of AC lung tumors. PMID:26010604

  15. GENETIC ASSOCIATION BETWEEN HUMAN CHITINASES AND LUNG FUNCTION IN COPD

    PubMed Central

    Aminuddin, F.; Akhabir, L.; Stefanowicz, D.; Paré, P.D.; Connett, J.E.; Anthonisen, N.R.; Fahy, J.V.; Seibold, M.A.; Burchard, E.G.; Eng, C.; Gulsvik, A.; Bakke, P.; Cho, M. H.; Litonjua, A.; Lomas, D.A.; Anderson, W. H.; Beaty, T.H.; Crapo, J.D.; Silverman, E.K.; Sandford, A.J.

    2013-01-01

    Two primary chitinases have been identified in humans – acid mammalian chitinase (AMCase) and chitotriosidase (CHIT1). Mammalian chitinases have been observed to affect the host’s immune response. The aim of this study was to test for association between genetic variation in the chitinases and phenotypes related to Chronic Obstructive Pulmonary Disease (COPD). Polymorphisms in the chitinase genes were selected based on previous associations with respiratory diseases. Polymorphisms that were associated with lung function level or rate of decline in the Lung Health Study (LHS) cohort were analyzed for association with COPD affection status in four other COPD case-control populations. Chitinase activity and protein levels were also related to genotypes. In the Caucasian LHS population, the baseline forced expiratory volume in one second (FEV1) was significantly different between the AA and GG genotypic groups of the AMCase rs3818822 polymorphism. Subjects with the GG genotype had higher AMCase protein and chitinase activity compared with AA homozygotes. For CHIT1 rs2494303, a significant association was observed between rate of decline in FEV1 and the different genotypes. In the African American LHS population, CHIT1 rs2494303 and AMCase G339T genotypes were associated with rate of decline in FEV1. Although a significant effect of chitinase gene alleles was found on lung function level and decline in the LHS, we were unable to replicate the associations with COPD affection status in the other COPD study groups. PMID:22200767

  16. Human papillomavirus DNA in squamous cell carcinoma of the lung.

    PubMed Central

    Hirayasu, T; Iwamasa, T; Kamada, Y; Koyanagi, Y; Usuda, H; Genka, K

    1996-01-01

    AIM: To compare the incidence of squamous cell carcinoma (SCC) of the lung in Okinawa with that in Niigata on the mainland. METHODS: All patients presenting with SCC of the lung in Okinawa and Niigata in 1993 were included in the study. Diagnoses were confirmed by conventional histological examination of paraffin wax sections. Human papillomavirus (HPV) was detected by non-isotopic in situ hybridisation (NISH) and polymerase chain reaction (PCR) amplification with primers specific for the E6 and E7 regions of the HPV genome. PCR products were analysed by Southern and dot blotting. RESULTS: The incidence of well differentiated SCC of the lung was high in patients from Okinawa compared with moderately and poorly differentiated types, and compared with the incidence of SCC in patients from Niigata. This is despite similar patterns of age, sex (predominatly male), and smoking habit. More patients from Okinawa, however, were positive for HPV DNA by PCR (79%) and NISH (53%). Many patients haboured HPV types 6, 16, and 18. Only 30% of patients from Niigata were positive for HPV DNA by PCR and 20% by NISH. These patients all harboured one HPV type only. CONCLUSION: Surprisingly large numbers of patients from Okinawa were positive for HPV DNA. The detection of HPV DNA was strongly associated with well differentiated SCC. This was particularly true for HPV types 6 and 16. There was no correlation between either smoking and detection of HPV DNA, or smoking and histological differentiation. Images PMID:8943746

  17. Implications for Human Leukocyte Antigen Antibodies After Lung Transplantation

    PubMed Central

    Wang, Ziwei; Chen, Dong-Feng; Reinsmoen, Nancy L.; Finlen-Copeland, C. Ashley; Davis, W. Austin; Zaas, David W.; Palmer, Scott M.

    2013-01-01

    Background: Long-term survival after lung transplant is limited by the development of chronic and progressive airflow obstruction, a condition known as bronchiolitis obliterans syndrome (BOS). While prior studies strongly implicate cellular rejection as a strong risk factor for BOS, less is known about the clinical significance of human leukocyte antigen (HLA) antibodies and donor HLA-specific antibodies in long-term outcomes. Methods: A single-center cohort of 441 lung transplant recipients, spanning a 10-year period, was prospectively screened for HLA antibodies after transplant using flow cytometry-based methods. The prevalence of and predictors for HLA antibodies were determined. The impact of HLA antibodies on survival after transplant and the development of BOS were determined using Cox models. Results: Of the 441 recipients, 139 (32%) had detectable antibodies to HLA. Of these 139, 54 (39%) developed antibodies specific to donor HLA. The detection of posttransplant HLA antibodies was associated with BOS (HR, 1.54; P = .04) and death (HR, 1.53; P = .02) in multivariable models. The detection of donor-specific HLA antibodies was associated with death (HR, 2.42; P < .0001). The detection of posttransplant HLA antibodies was associated with pretransplant HLA-antibody detection, platelet transfusions, and the development of BOS and cytomegalovirus pneumonitis. Conclusions: Approximately one-third of lung transplant recipients have detectable HLA antibodies, which are associated with a worse prognosis regarding graft function and patient survival. PMID:23328795

  18. [The lungs in human immunodeficiency virus type 1 infection].

    PubMed

    Barić, D; Vrkić, L

    1997-01-01

    This report describes a case of two patients who were admitted to the Zadar hospital and according to clinical symptoms directed to the Department of Lung Diseases. Both patients were temporarily employed abroad. It has been established that they were infected with human immunodeficiency virus type 1 (HIV-1). One of the patients has been moved to the Department of Infectious Diseases and later to Zagreb, while the other has returned abroad. On admission to the hospital of the Zadar Medical Center none of them answered the question about being engaged in risky behavior. In 1990 there were 699 registered patients hospitalized and 745 registered in the protocol of the Outpatient Clinic of the Department of Lung Diseases. 0.069% of patients were HIV-1-infected. In 1991, there were 520 hospitalized and 453 outpatients, whereas 0.102% were HIV-1-infected and registered subjects. It must be pointed out that these are only numbers of registration and not subjects, because there were patients who were examined or hospitalized twice or more times during the corresponding calendar year. The aim of this study was to point to a new differentially-diagnostic problem present especially at the Department of Lung Diseases after AIDS has become part of our reality. There still remains a problem in regard to detection of HIV-1 seropositivity in patients at departments with opportunistic infections such as tuberculosis.

  19. Cellular morphometry of the bronchi of human and dog lungs

    SciTech Connect

    Robbins, E.S.

    1991-09-01

    One hundred and forty-seven bronchial samples (generations 3--6) from 66 patients (62 usable; 36 female, 26 male; median age 61) have been dissected by generation from fixed surgical lung specimens obtained after the removal of pathological lesions. In addition, one hundred and fifty-six mongol dog bronchi (generations 2--6) dissected from different lobes of 26 dog lungs have also been similarly prepared. One hundred and twenty-seven human samples have been completely processed for electron microscopy and have yielded 994 electron micrographs of which 655 have been entered into the Computerized Stereological Analysis System (COSAS) and been used for the measurement of the distances of basal and mucous cell nuclei to the epithelial free surface. Similarly 328 micrographs of dog epithelium from 33 bronchial samples have been used to measure the distances of basal and mucous cell nuclei to the epithelial free surface and have been entered into COSAS. Using the COSAS planimetry program, we continue to expand our established data bases which describe the volume density and nuclear numbers per electron micrograph for 5 cell types of the human bronchial epithelial lining of men and women, as well as smokers, non-smokers and ex-smokers and similar parameters for the same 5 epithelial cell types of dog bronchi. Our micrographs of human bronchial epithelium have allowed us to analyze the recent suggestion that the DNA of lymphocytes may be subject to significant damage from Rn progeny while within the lung. Since the last progress report three papers have been submitted for publication. 17 refs., 4 tabs.

  20. Cytoprotective and Antioxidant Effects of Steen Solution on Human Lung Spheroids and Human Endothelial Cells.

    PubMed

    Pagano, F; Nocella, C; Sciarretta, S; Fianchini, L; Siciliano, C; Mangino, G; Ibrahim, M; De Falco, E; Carnevale, R; Chimenti, I; Frati, G

    2017-07-01

    Respiratory diseases represent a major healthcare burden worldwide. Lung transplantation (LTx) is the "gold standard" for end-stage patients, strongly limited by shortage of available/suitable donor lungs. Normothermic ex vivo lung perfusion (EVLP) has significantly increased the number of lungs suitable for transplantation. Steen solution is used for EVLP, but the mechanisms involved in its beneficial properties remain to be clarified. We investigated the effects of Steen solution in an in vitro protocol of cold starvation and normothermic recovery on human lung spheroids, named pneumospheres (PSs), containing epithelial/basal cells, and on endothelial human umbilical vein endothelial cells (HUVEC). Steen solution significantly preserved the viability of PSs, reduced reactive oxygen species (ROS) release by PSs and HUVECs, decreased NADPH-oxidase (NOX) activity in PSs, and reduced inflammatory cytokines expression levels in HUVECs. Steen solution was able to specifically reduce NADPH oxidase 2 (NOX2) isoform activation, particularly in PSs, as detected by soluble-NOX2 peptide and p47-phosphorylation. Interestingly, a specific NOX2 inhibitor could partly mimic the pro-survival effect of Steen on PSs. We provide the first evidence that Steen solution can preserve lung epithelial/progenitor cells viability partially through NOX2 downregulation, and exert antioxidant effects on parenchymal cells, with consequent ROS reduction. These results suggest that NOX2 inhibition might be an additional strategy to reduce cellular damage during LTx procedures. © 2017 The American Society of Transplantation and the American Society of Transplant Surgeons.

  1. Gene Expression Analysis to Assess the Relevance of Rodent Models to Human Lung Injury.

    PubMed

    Sweeney, Timothy E; Lofgren, Shane; Khatri, Purvesh; Rogers, Angela J

    2017-08-01

    The relevance of animal models to human diseases is an area of intense scientific debate. The degree to which mouse models of lung injury recapitulate human lung injury has never been assessed. Integrating data from both human and animal expression studies allows for increased statistical power and identification of conserved differential gene expression across organisms and conditions. We sought comprehensive integration of gene expression data in experimental acute lung injury (ALI) in rodents compared with humans. We performed two separate gene expression multicohort analyses to determine differential gene expression in experimental animal and human lung injury. We used correlational and pathway analyses combined with external in vitro gene expression data to identify both potential drivers of underlying inflammation and therapeutic drug candidates. We identified 21 animal lung tissue datasets and three human lung injury bronchoalveolar lavage datasets. We show that the metasignatures of animal and human experimental ALI are significantly correlated despite these widely varying experimental conditions. The gene expression changes among mice and rats across diverse injury models (ozone, ventilator-induced lung injury, LPS) are significantly correlated with human models of lung injury (Pearson r = 0.33-0.45, P < 1E(-16)). Neutrophil signatures are enriched in both animal and human lung injury. Predicted therapeutic targets, peptide ligand signatures, and pathway analyses are also all highly overlapping. Gene expression changes are similar in animal and human experimental ALI, and provide several physiologic and therapeutic insights to the disease.

  2. Radiographic Comparison of Human Lung Shape During Normal Gravity and Weightlessness

    NASA Technical Reports Server (NTRS)

    Michels, D. B.; Friedman, P. J.; West, J. B.

    1979-01-01

    Chest radiographs in five seated normal volunteers at 1 G and 0 G were made with a view toward comparing human lung shape during normal gravity and weightlessness. Lung shape was assessed by measuring lung heights and widths in upper, middle and lower lung regions. No significant differences were found between any of the 1-G and 0-G measurements, although there was a slight tendency for the lung to become shorter and wider at 0 G. The evidence that gravity causes regional differences in ventilation by direct action on the lung is consistent with the theoretical analysis of West and Matthews (1972).

  3. Polarized {sup 3}He{sup −} ion source with hyperfine state selection

    SciTech Connect

    Dudnikov, V.; Morozov, V.; Dudnikov, A.

    2015-04-08

    High beam polarization is essential to the scientific productivity of a collider. Polarized {sup 3}He ions are an essential part of the nuclear physics programs at existing and future ion-ion and electron-ion colliders such as BNL's RHIC and eRHIC and JLab's ELIC. Ion sources with performance exceeding that achieved today are a key requirement for the development of these next generation high-luminosity high-polarization colliders. The development of high-intensity high-brightness arc-discharge ion sources at the Budker Institute of Nuclear Physics (BINP) has opened up an opportunity for realization of a new type of a polarized {sup 3}He{sup −} ion source. This report discusses a polarized {sup 3}He{sup −} ion source based on the large difference of extra-electron auto-detachment lifetimes of the different {sup 3}He{sup −} ion hyperfine states. The highest momentum state of 5/2 has the largest lifetime of τ ∼ 350 µs while the lower momentum states have lifetimes of τ ~ 10 µs. By producing {sup 3}He{sup −} ion beam composed of only the |5/2, ±5/2> hyperfine states and then quenching one of the states by an RF resonant field, {sup 3}He{sup −} beam polarization of 90% can be achieved. Such a method of polarized {sup 3}He{sup −} production has been considered before; however, due to low intensities of the He{sup +} ion sources existing at that time, it was not possible to produce any interesting intensity of polarized {sup 3}He{sup −} ions. The high-brightness arc-discharge ion source developed at BINP can produce a high-brightness {sup 3}He{sup +} beam with an intensity of up to 2 A allowing for selection of up to ∼1-4 mA of {sup 3}He{sup −} ions with ∼90% polarization. The high gas efficiency of an arc-discharge source is important due to the high cost of {sup 3}He gas. Some features of such a PIS as well as prototype designs are considered. An integrated {sup 3}He{sup −} ion source design providing high beam polarization could be

  4. Triple oxygen isotopic composition of the high-3He/4He mantle

    NASA Astrophysics Data System (ADS)

    Starkey, N. A.; Jackson, C. R. M.; Greenwood, R. C.; Parman, S.; Franchi, I. A.; Jackson, M.; Fitton, J. G.; Stuart, F. M.; Kurz, M.; Larsen, L. M.

    2016-03-01

    Measurements of Xe isotope ratios in ocean island basalts (OIB) suggest that Earth's mantle accreted heterogeneously, and that compositional remnants of accretion are sampled by modern, high-3He/4He OIB associated with the Icelandic and Samoan plumes. If so, the high-3He/4He source may also have a distinct oxygen isotopic composition from the rest of the mantle. Here, we test if the major elements of the high-3He/4He source preserve any evidence of heterogeneous accretion using measurements of three oxygen isotopes on olivine from a variety of high-3He/4He OIB locations. To high precision, the Δ17O value of high-3He/4He olivines from Hawaii, Pitcairn, Baffin Island and Samoa, are indistinguishable from bulk mantle olivine (Δ17OBulk Mantle - Δ17OHigh 3He/4He olivine = -0.002 ± 0.004 (2 × SEM)‰). Thus, there is no resolvable oxygen isotope evidence for heterogeneous accretion in the high-3He/4He source. Modelling of mixing processes indicates that if an early-forming, oxygen-isotope distinct mantle did exist, either the anomaly was extremely small, or the anomaly was homogenised away by later mantle convection. The δ18O values of olivine with the highest 3He/4He ratios from a variety of OIB locations have a relatively uniform composition (∼5‰). This composition is intermediate to values associated with the depleted MORB mantle and the average mantle. Similarly, δ18O values of olivine from high-3He/4He OIB correlate with radiogenic isotope ratios of He, Sr, and Nd. Combined, this suggests that magmatic oxygen is sourced from the same mantle as other, more incompatible elements and that the intermediate δ18O value is a feature of the high-3He/4He mantle source. The processes responsible for the δ18O signature of high-3He/4He mantle are not certain, but δ18O-87Sr/86Sr correlations indicate that it may be connected to a predominance of a HIMU-like (high U/Pb) component or other moderate δ18O components recycled into the high-3He/4He source.

  5. The heavy ion composition in 3HE-rich solar flares

    NASA Technical Reports Server (NTRS)

    Mason, G. M.; Reames, D. V.; Hovestadt, D.; Vonrosenvinge, T. T.

    1985-01-01

    The 3He-rich flares show a tendency to be enriched in heavy ions, and that this enrichment covers the charge range through Fe. The discovery of this association was responsible, in part, for the discarding of 3He enrichment models which involved spallation or thermonuclear reactions, since such models were unable to produce heavy nuclei enhancement. Results of a survey of heavy nucleus abundances observed in 66 3He-rich flares which occurred over the period October 1978 to June 1982 are presented.

  6. Recent advances of polarized {sup 3}He target at Jefferson Lab

    SciTech Connect

    Qiang Yi

    2011-10-24

    Polarized {sup 3}He targets have been widely used in nuclear and particle physics experiments to study neutron structure in the spin degree of freedom, as most of the {sup 3}He spin is carried by the unpaired neutron. The Spin-Exchange Optical Pumping (SEOP) process is used in Jefferson Lab Hall A to polarize its {sup 3}He target. In recent years, both the performance and corresponding polarimetry of such a target have been greatly improved. Several experiments recently carried out in Hall A have achieved record high figure of merit using this target.

  7. Recent advances in spin-exchange pumped polarized 3He target technology

    NASA Astrophysics Data System (ADS)

    Smith, T. B.; Chupp, T. E.; Coulter, K. P.; Welsh, R. C.

    1998-02-01

    We have produced long lifetime 3He spin-exchange cells from Corning 7056 glass. The lifetimes of single cells have approached the 3He 3He bulk-limited lifetime (250 h at a density of 8 × 10 19 cm -3, (3 amagats)). Corning 7056 glass has the advantage of being a much easier glass for the glassblower to work, allowing for more complex cell designs. In our experiments at Michigan and at SLAC, we have implemented laser diode arrays for spin-exchange optical pumping. In particular, for experiment E154 at SLAC, we achieved high polarizations in high-density 3He targets using laser diode arrays.

  8. Investigation of the {sup 3}He wave function by quasifree scattering

    SciTech Connect

    Jones, C.E.; Hansen, J.O.; Bloch, C.

    1995-08-01

    The analysis of the data from the CE25 experiment at IUCF, which measured the target and beam analyzing powers and the spin correlation parameter in {sup 3}He(p,2p) and {sup 3}He(p,pn) quasielastic scattering, is nearing completion. At low missing momentum, the extracted polarization of the neutron and proton in {sup 3}He are consistent with Faddeev calculations. Two papers, one reporting the physics results and one describing the experiment, were published. The data from this experiment indicates that for q {>=} 500 MeV/c the plane wave impulse approximation is valid.

  9. Discriminating Acquisition of 15-MeV Protons from D-3He Fusion Reaction in LHD

    NASA Astrophysics Data System (ADS)

    Watanabe, Tsuguhiro; Miyazawa, Junichi; Yamada, Hiroshi; Murakami, Sadayoshi; Masuzaki, Suguru; Osakabe, Masaki; Isobe, Mitsutaka; Tokitani, Masayuki; Motojima, Osamu

    Discriminating acquisition of 15-MeV protons is possible in LHD D-3He experiments (D+ beam to 3He plasma), due to the nonaxisymmetric structure of the magnetic field and the ultra-high energy of the fusion products. The collisionless orbits of D-3He fusion products are studied numerically in the standard magnetic field configuration of LHD. Three sets of fusion product acquisition systems are installed in LHD and numerical computations show the possibility of discriminating between fusion products and plasma particles. The acquisition rate of 15-MeV protons is expected to be in the range of 12 ˜ 28 %.

  10. Development of D+3He Fusion Electric Thrusters and Power Supplies for Space

    NASA Astrophysics Data System (ADS)

    Morse, Thomas M.

    1994-07-01

    Development of D+3He Fusion Electric Thrusters (FET) and Power Supplies (FPS) should occur at a lunar base because of the following: availability of helium-3, a vacuum better than on Earth, low K in shade reachable by radiant cooling, supply of ``high temp'' superconducting ceramic-metals, and a low G environment. The early FET will be much smaller than an Apollo engine, with specific impulse of 10,000-100,000-s. Solar power and low G will aid early development. To counter the effect of low G on humans, centrifuges will be employed for sleeping and resting. Work will be done by telerobotic view control. The FPS will be of comparable size, and will generate power mainly by having replaceable rectennas, resonant to the fusion synchrotron radiation. FPSs are used for house keeping power and initiating superconduction. Spaceships will carry up to ten FETs and two FPSs. In addition to fusion fuel, the FET will inject H or Li low mass propellant into the fusion chamber. Developing an FET would be difficult on Earth. FET spaceships will park between missions in L1, and an FET Bus will fetch humans/supplies from Moon and Earth. Someday FETs, with rocket assist, will lift spaceships from Earth, and make space travel to planets far cheaper, faster, and safer, than at present. Too long a delay due to the space station, or the huge cost of getting into space by current means, will damage the morale of the space program.

  11. Cancer-associated loss of TARSH gene expression in human primary lung cancer.

    PubMed

    Terauchi, Kunihiko; Shimada, Junichi; Uekawa, Natsuko; Yaoi, Takeshi; Maruyama, Mitsuo; Fushiki, Shinji

    2006-01-01

    We have previously identified mouse Tarsh as one of the cellular senescence-related genes and showed the loss of expression of TARSH mRNA in four human lung cancer cell lines. TARSH is a presumptive signal transduction molecule interacting with NESH, which is implicated to have some roles in lung cancer metastasis. The amplification of complete ORF-encoding TARSH cDNA was done with reverse transcription-PCR. Northern blotting was carried out using TARSH cDNA probes. To clarify the relationship between TARSH and lung cancer, we quantified TARSH mRNA expression in 15 human lung cancer cell lines and 32 primary non-small cell lung cancers. We first determined the complete ORF-encoding cDNA sequence which is expressed in the human lung. On the Northern hybridization analysis, TARSH was strongly expressed in the human lung. The expression of TARSH mRNA is remarkably downregulated in all the lung cancer cell lines examined. Furthermore, TARSH expression was significantly low in all of the tumor specimens when compared to the expression in corresponding non-neoplastic lung tissue specimens. The cancer-associated transcriptional inactivation of TARSH suggests that TARSH could be used as a biomarker for lung cancer development as well as a molecular adjunct for lung carcinogenesis in human.

  12. IMP3 Predicts Invasion and Prognosis in Human Lung Adenocarcinoma.

    PubMed

    Yan, Jinhai; Wei, Qingzhu; Jian, Wenjing; Qiu, Bo; Wen, Jing; Liu, Jianghuan; Fu, Bo; Zhou, Xinhua; Zhao, Tong

    2016-02-01

    Insulin-like growth factor II mRNA-binding protein 3 (IMP3) is an oncofetal protein associated with several aggressive and advanced cancers. Whether IMP3 can predict invasion, and prognosis in patients with human lung adenocarcinoma (LAC) remains unclear. Ninety-five LAC and 75 non-tumor lung tissue samples were included in a tissue microarray. IMP3 expression was assessed by immunohistochemical examination. Correlation between IMP3 expression levels, clinicopathological characteristics, and overall prognosis was evaluated. In a separate in vitro study, RNA interference method was applied for knockdown of IMP3 gene in human LAC cell lines. Invasive potential of LAC cells was then evaluated by transwell migration assay. IMP3 immunoreactivity was observed in 39 out of 95 (41.1 %) LAC patients, but not in non-tumor lung tissues. IMP3 expression levels were closely associated with histological grade (P = 0.037), TNM stage (P = 0.034), and lymph node metastasis (P = 0.011). Patients presenting with positive IMP3 expression (P = 0.000), an advanced TNM stage (P = 0.000), and lymph node metastasis (P = 0.001) had a worse overall survival, compared to those lacking these characteristics. Both IMP3 expression (hazard ratio [HR], 2.310; 95 % confidence interval [CI] 1.192-4.476; P = 0.013) and TNM stage (HR 2.338; 95 % CI 1.393-3.925; P = 0.001) were independent predictors of poor prognosis. The invasive potential of LAC cells was significantly inhibited by IMP3 knockdown. IMP3 appears to play an important role in tumor invasion in patients with LAC and may serve as a useful prognostic biomarker in these patients.

  13. Experimental study and nuclear model calculations of 3He-induced nuclear reactions on zinc

    NASA Astrophysics Data System (ADS)

    Al-Abyad, M.; Mohamed, Gehan Y.; Ditrói, F.; Takács, S.; Tárkányi, F.

    2017-05-01

    Excitation functions of 3He -induced nuclear reactions on natural zinc were measured using the standard stacked-foil technique and high-resolution gamma-ray spectrometry. From their threshold energies up to 27MeV, the cross-sections for natZn (3He, xn) 69Ge, natZn(3He, xnp) 66,67,68Ga, and natZn(3He, x)62,65Zn reactions were measured. The nuclear model codes TALYS-1.6, EMPIRE-3.2 and ALICE-IPPE were used to describe the formation of these products. The present data were compared with the theoretical results and with the available experimental data. Integral yields for some important radioisotopes were determined.

  14. Recent advancements of wide-angle polarization analysis with 3He neutron spin filters

    NASA Astrophysics Data System (ADS)

    Chen, W. C.; Gentile, T. R.; Ye, Q.; Kirchhoff, A.; Watson, S. M.; Rodriguez-Rivera, J. A.; Qiu, Y.; Broholm, C.

    2016-09-01

    Wide-angle polarization analysis with polarized 3He based neutron spin filters (NSFs) has recently been employed on the Multi-Axis Crystal Spectrometer (MACS) at the National Institute of Standards and Technology Center for Neutron Research (NCNR). Over the past several years, the apparatus has undergone many upgrades to address the fundamental requirements for wide angle polarization analysis using spin exchange optical pumping based 3He NSFs. In this paper, we report substantial improvements in the on-beam-line performance of the apparatus and progress toward routine user capability. We discuss new standard samples used for 3He NSF characterization and the flipping ratio measurement on MACS. We further discuss the management of stray magnetic fields produced by operation of superconducting magnets on the MACS instrument, which can significantly reduce the 3He polarization relaxation time. Finally, we present the results of recent development of horseshoe-shaped wide angle cells.

  15. Progress Towards the Detection of Faraday Rotation on Spin Polarized 3He

    NASA Astrophysics Data System (ADS)

    Abney, Josh; Broering, Mark; Korsch, Wolfgang

    2016-03-01

    Off-resonance Faraday rotation can offer a new method to monitor the nuclear spin polarization of a dense 3He target and gain access to new information about the magnetic polarizability of the 3He nucleus. The interaction of the polarization state of light with the nuclear spin of the helium atom is very weak and has never been detected. A sensitive triple modulation technique has been developed which can detect the expected rotation angle on the order of 100 nrad. Once a Faraday rotation signal is observed, the next step is to separate the magnetic and electric contributions to the rotation by utilizing their different frequency dependencies. Recent studies involved optimizing several parameters which impact 3He target polarization. Progress towards detecting nuclear spin optical rotation on 3He will be reported. This research is supported by DOE Grant DE-FG02-99ER41101.

  16. The enigmatic high 3He/4He mantle: Characteristics and Origins. (Invited)

    NASA Astrophysics Data System (ADS)

    Jackson, M. G.

    2009-12-01

    Noble gas isotopes measured in some oceanic island basalts (OIBs) exhibit ratios that are associated with the solar wind and the atmosphere of Jupiter, suggesting that the lavas tap portions of an ancient reservoir that still resides in the Earth’s mantle [e.g., 1]. High 3He/4He, as seen in the sources of some OIBs, can therefore serve as a powerful indicator for tracing ancient signatures that have survived in the Earth’s interior. However, the storage mechanisms and reasons for long-term survival of the high 3He/4He signature in the Earth’s convecting mantle are poorly understood. One important observation is that high 3He/4He lavas have 143Nd/144Nd ratios that are higher than chondrites, suggesting that they were derived from a mantle reservoir that suffered ancient depletion. The association of primitive, high 3He/4He with depleted, nonprimitive 143Nd/144Nd in OIBs is not straightforward and a number of models have been developed to resolve this apparent complexity [e.g., 2,3,4,5,6]. It is also becoming apparent that the high 3He/4He reservoir is heterogeneous. High 3He/4He (>30 times atmospheric) lavas from Hawaii, Iceland and Galapagos have more depleted 143Nd/144Nd (0.51294-0.51297) than lavas with similarly high 3He/4He from Samoa (0.51283). In fact, the highest 3He/4He sample from each southern hemisphere high 3He/4He hotspot (FOZO-A, austral) exhibits lower 143Nd/144Nd ratios their northern hemisphere (FOZO-B, boreal) counterparts. The mechanism for this separation is unknown, but it is similar in spatial scale to the DUPAL anomaly, a globe-encircling feature of isotopic enrichment observed primarily in southern hemisphere OIBs. With the exception of Baffin Is. picrites [7], high 3He/4He OIBs also exhibit evidence for Ti, Ta, and Nb (TITAN) enrichment relative to low 3He/4He OIBs. This was interpreted as the result of addition of refractory, rutile-bearing eclogite to a peridotitic high 3He/4He reservoir [8]. This hypothesis is supported by the

  17. On Feasibility of Absolute Measurements of Ion Temperature in D-3He Fusion Plasma

    NASA Astrophysics Data System (ADS)

    Voronchev, Victor T.; Nakao, Yasuyuki

    2003-05-01

    We investigate a possibility of using γ-ray-generating nuclear reactions for absolute measurements of bulk ion temperature in D-3He fusion plasma. Radiative capture processes between fuel ions and specific γ-ray modes of nuclear reactions induced by a small admixture of 6Li are examined for such an application. It is shown that ion temperature can be determined by comparative measurements of the respective γ-ray yields. An essential point of the method proposed is that any fluctuations of plasma parameters during the measurements do not affect accuracy of temperature probe. Nuclear reactions 3He(3He,γ)6Be and 6Li(3He,γp)8Be are suggested for diagnostics for the first time. Necessary data on reaction cross sections and Maxwellian rate parameters are calculated.

  18. Core Plasma Characteristics of a Spherical Tokamak D-3He Fusion Reactor

    NASA Astrophysics Data System (ADS)

    Shi, Bingren

    2005-04-01

    The magnetic fusion reactor using the advanced D-3He fuels has the advantage of much less-neutron productions so that the consequent damages to the first wall are less serious. If the establishment of this kind of reactor becomes realistic, the exploration of 3He on the moon will be largely motivated. Based on recent progresses in the spherical torus (ST) research, we have physically designed a D-3He fusion reactor using the extrapolated results from the ST experiments and also the present-day tokamak scaling. It is found that the reactor size significantly depends on the wall reflection coefficient of the synchrotron radiation and of the impurity contaminations. The secondary reaction between D-D that promptly leads to the D-T reaction producing 14 MeV neutrons is also estimated. Comparison of this D-3He ST reactor with the D-T reactor is made.

  19. Effect of ^3He impurity on the supersolid transition of ^4He

    NASA Astrophysics Data System (ADS)

    Kim, Eunseong; Chan, Moses H. W.

    2006-03-01

    The supersolid phase of ^4He was revealed by a series of torsional oscillator experiments.[1] One of the most intriguing features of the supersolid transition is the broadening of the transition and the enhancement of Tc by the addition of extremely small amount of ^3He impurity. This effect is very different from that in superfluid film and that in `bulk' superfluid helium. We have investigated the^ influence of ^3He on the supersolid transition by systematically diluting isotopically-pure ^4He (^3He impurity less than 0.3ppb) with ^3He. [1] E. Kim and M. H. W. Chan, Science 305, 1941 (2004); Nature 425, 227 (2004); J. Low Temp. Phys. 138, 859 (2005)

  20. Sol-gel coatings for high pressure polarized ^3He nuclear targets

    NASA Astrophysics Data System (ADS)

    Deur, Alexandre; Cates, Gordon D.; Chaput, Julien; Singh, Jaideep; Tobias, William A.

    2001-11-01

    Sol-gel coated glass cells have been shown to exhibit longitudinal lifetimes T1 in excess of 350 hours for ^3He that is polarized by spin-exchange optical pumping.( Ming F. Hsu shape et al, Appl. Phys. Lett.) series 77 (2000) 2069. The sol-gel technique was designed to minimize spin-relaxation due to wall collisions so that only dipole-dipole interactions between colliding ^3He atoms dominate in the relaxation process. Until now, sol-gel technology has not been applied to high pressure ^3He gas targets used in nuclear scattering experiments. Latest developments on incorporating the sol-gel technique in the production of these ^3He targets will be presented.

  1. Decreased Laminin Expression by Human Lung Epithelial Cells and Fibroblasts Cultured in Acellular Lung Scaffolds from Aged Mice.

    PubMed

    Godin, Lindsay M; Sandri, Brian J; Wagner, Darcy E; Meyer, Carolyn M; Price, Andrew P; Akinnola, Ifeolu; Weiss, Daniel J; Panoskaltsis-Mortari, Angela

    2016-01-01

    The lung changes functionally and structurally with aging. However, age-related effects on the extracellular matrix (ECM) and corresponding effects on lung cell behavior are not well understood. We hypothesized that ECM from aged animals would induce aging-related phenotypic changes in healthy inoculated cells. Decellularized whole organ scaffolds provide a powerful model for examining how ECM cues affect cell phenotype. The effects of age on ECM composition in both native and decellularized mouse lungs were assessed as was the effect of young vs old acellular ECM on human bronchial epithelial cells (hBECs) and lung fibroblasts (hLFs). Native aged (1 year) lungs demonstrated decreased expression of laminins α3 and α4, elastin and fibronectin, and elevated collagen, compared to young (3 week) lungs. Proteomic analyses of decellularized ECM demonstrated similar findings, and decellularized aged lung ECM contained less diversity in structural proteins compared to young ECM. When seeded in old ECM, hBECs and hLFs demonstrated lower gene expression of laminins α3 and α4, respectively, as compared to young ECM, paralleling the laminin deficiency of aged ECM. ECM changes appear to be important factors in potentiating aging-related phenotypes and may provide clues to mechanisms that allow for aging-related lung diseases.

  2. Decreased Laminin Expression by Human Lung Epithelial Cells and Fibroblasts Cultured in Acellular Lung Scaffolds from Aged Mice

    PubMed Central

    Godin, Lindsay M.; Sandri, Brian J.; Wagner, Darcy E.; Meyer, Carolyn M.; Price, Andrew P.; Akinnola, Ifeolu; Weiss, Daniel J.; Panoskaltsis-Mortari, Angela

    2016-01-01

    The lung changes functionally and structurally with aging. However, age-related effects on the extracellular matrix (ECM) and corresponding effects on lung cell behavior are not well understood. We hypothesized that ECM from aged animals would induce aging-related phenotypic changes in healthy inoculated cells. Decellularized whole organ scaffolds provide a powerful model for examining how ECM cues affect cell phenotype. The effects of age on ECM composition in both native and decellularized mouse lungs were assessed as was the effect of young vs old acellular ECM on human bronchial epithelial cells (hBECs) and lung fibroblasts (hLFs). Native aged (1 year) lungs demonstrated decreased expression of laminins α3 and α4, elastin and fibronectin, and elevated collagen, compared to young (3 week) lungs. Proteomic analyses of decellularized ECM demonstrated similar findings, and decellularized aged lung ECM contained less diversity in structural proteins compared to young ECM. When seeded in old ECM, hBECs and hLFs demonstrated lower gene expression of laminins α3 and α4, respectively, as compared to young ECM, paralleling the laminin deficiency of aged ECM. ECM changes appear to be important factors in potentiating aging-related phenotypes and may provide clues to mechanisms that allow for aging-related lung diseases. PMID:26954258

  3. Metabolism kinetics of beclomethasone propionate esters in human lung homogenates.

    PubMed

    Foe, K; Cutler, D J; Brown, K F; Seale, J P

    2000-08-01

    The purposes of this study were to characterize the kinetics of beclomethasone dipropionate (BDP) and its 17-monopropionate ester (17-BMP) in human lung 1000g supernatant (HLu) at 37 degrees C, and to analyze the interindividual variability in the metabolism of BDP in HLu. The concentrations of BDP and its metabolites were determined by HPLC with UV detection at 242 nm. Kinetics of BDP and 17-BMP decomposition were characterized by least-squares fitting of rate equations. The active metabolite 17-BMP was rapidly formed following the incubation of BDP in HLu. Kinetics of BDP and 17-BMP in HLu were nonlinear owing to product inhibition and enzyme saturation. A model taking into account the product inhibition provides a kinetic basis for understanding the in vivo behavior of BDP and its metabolites in human lung. There was approximately a 3.5-fold difference in the initial half-life of BDP in HLu observed in seven subjects. An effective activation of BDP was demonstrated in HLu through the rapid formation of 17-BMP. Kinetics of BDP and 17-BMP in HLu were well characterized by the nonlinear kinetic model. Interindividual difference in the initial half-life of BDP was due mainly to esterase metabolizing activity rather than binding affinity.

  4. The Triple Oxygen Isotopic Composition of High 3He/4He Mantle

    NASA Astrophysics Data System (ADS)

    Jackson, C.; Parman, S. W.; Starkey, N.; Greenwood, R.; Franchi, I.; Jackson, M. G.; Fitton, J. G.; Stewart, F. M.; Larsen, L. M.

    2015-12-01

    Measurements of Xe isotope ratios in ocean island basalts (OIB) suggest that Earth's mantle accreted heterogeneously, and that compositional remnants of accretion are sampled by modern, high-3He/4He OIB associated with the Icelandic and Samoan plumes [1]. If so, the high-3He/4He source may also have a distinct oxygen isotopic composition from the rest of the mantle. Here, we test if the major elements of the high-3He/4He source preserve any evidence of heterogeneous accretion using measurements of three oxygen isotopes on olivine from a variety of high-3He/4He OIB locations. To high precision, the ∆17O value of high-3He/4He olivines from Hawaii, Pitcairn, Baffin Island and Samoa, are indistinguishable from bulk mantle olivine (Δ17OBulk Mantle - Δ17OHigh 3He/4He olivine = -0.002 ± 0.004 (2 x SEM) ‰). Thus, there is no resolvable oxygen isotope evidence for heterogeneous accretion in the high-3He/4He source. Modelling of mixing processes indicates that if an early-forming, oxygen-isotope distinct mantle did exist, either the anomaly was extremely small, or the anomaly was homogenised away by later mantle convection. The δ18O values of olivine with the highest 3He/4He ratios from a variety of OIB locations have a relatively uniform composition (~5 ‰). This composition is intermediate to values associated with the depleted MORB mantle and the average mantle. Similarly, δ18O values of olivine from high-3He/4He OIB correlate with radiogenic isotope ratios of He, Sr, and Nd. Combined, this suggests that oxygen remains coupled to the more incompatible elements during melt production and migration and that the intermediate δ18O value is a feature of the mantle source. The processes responsible for the δ18O signature of high-3He/4He mantle are not certain, but δ18O-87Sr/86Sr correlations indicate that it may be connected to a predominance of a HIMU-like (high U/Pb) component or other moderate δ18O components recycled into the high-3He/4He source. [1] S

  5. Preservation of extraterrestrial 3He in 480-Ma-old marine limestones.

    PubMed

    Patterson, D B; Farley, K A; Schmitz, B

    1998-11-01

    We have measured the helium abundance and isotopic composition of a suite of Lower Ordovician marine limestones and associated fossil meteorites from Kinnekulle, Sweden. Limestone 3He/4He ratios as high as 11.5 times the atmospheric value in fused samples and up to 23 times atmospheric in a single step-heat fraction indicate the presence of extraterrestrial helium, and demonstrate that at least a fraction of the extraterrestrial 3He carried by interplanetary dust particles must be retained against diffusive and diagenetic losses for up to 480 Ma. The carrier phase has not been identified but is not magnetic. Extrapolation of high-temperature 3He diffusivities in these sediments is consistent with strong retention of extraterrestrial 3He under ambient Earth-surface conditions. Combination of the observed helium concentrations with sedimentation rates estimated from conodont biostratigraphy suggest that the flux of extraterrestrial 3He in the Early Ordovician was about 0.5 x 10(-12) cm3 STP cm-2 ka-1, ignoring potential post-deposition helium loss. This value is indistinguishable from the average 3He flux estimated for the Cenozoic Era. In contrast, previous studies of fossil meteorites, Ir abundances, and Os isotopic ratios in the limestone suggest that the total accretion rate of extraterrestrial material during the studied interval was at least an order of magnitude higher than the Cenozoic average. This disparity may reflect significant post-depositional loss of 3He from IDPs within these old limestones; if so, the match between the Ordovician flux and the Cenozoic average would be fortuitous. Alternatively, the size distribution of infalling objects during the Early Ordovician may have been enriched only in extraterrestrial material too large to retain 3He during atmospheric entry heating (> approximately 30 micrometers). The fossil meteorites themselves also preserve extraterrestrial helium. Meteorite 3He concentrations of 2 to 9 x 10(-12) cm3 STP g-1 are

  6. Cellular morphometry of the bronchi of human and dog lungs

    SciTech Connect

    Robbins, E.S.

    1992-09-01

    Quantitative data of the human bronchial epithelial cells at possible risk for malignant transformation in lung cancer is crucial for accurate radon dosimetry and risk analysis. The locations and other parameters of the nuclei which may be damaged by [alpha] particles must be determined and compared in different airway generations, among smokers, non-smokers and ex-smokers, between men and women and in people of different ages. This proposal includes extended morphometric studies on electron micrographs of human epithelium of defined airway generations and in parallel on electron micrographs of the dog bronchial lining. The second part of this proposal describes studies to quantitate the cycling bronchial epithelial population(s) using proliferation markers and immunocytochemistry on frozen and paraffin sections and similar labeling of isolated bronchial epithelial cells sorted flow cytometry.

  7. Prediction of reentrant wetting of [sup 3]He-[sup 4]He mixtures on cesium

    SciTech Connect

    Pettersen, M.S. ); Saam, W.F. )

    1993-02-01

    The authors examine the effect of [sup 3]He impurities on the wetting behavior of [sup 4]He on cesium, predicting a phase diagram which includes reentrant wetting transitions. This phase diagram is shown to be very sensitive to effects such as a theoretically predicted bound state of [sup 3]He at the liquid-cesium interface, and the contact angle may be sensitive to interesting temperature dependences of the helium-cesium surface tension resulting from surface rotons or Rayleigh waves.

  8. SANS study of phase separation in solid {sup 3}He-{sup 4}He

    SciTech Connect

    Koster, J.P.; Nagler, S.E.; Adams, E.D.; Wignall, G.D.

    1994-12-31

    Small angle neutron scattering has been used to study phase separation in a quantum alloy, solid {sup 3}He{sub x}-{sup 4}He{sub 1{minus}x}. The onset of phase separation is marked by a dramatic increase in the measured scattering. A simple interpretation of the results suggests that the late-stage phase separation kinetics are dominated by an increase in the concentration of {sup 3}He atoms in preexisting precipitate regions.

  9. Meson exchange currents for nuclear muon capture by {sup 3}He

    SciTech Connect

    Congleton, J.G.; Truhlik, E.

    1995-05-10

    We have calculated exchange corrections for nuclear muon capture by {sup 3}He using the hard pion method for the currents and wavefunctions for {sup 3}He and {sup 3}H found by the coupled rearrangement channel method. The result for the rate (triton asymmetry) has an uncertainty of 3% (1%) due mainly to the uncertainty in the value of {ital f}{sub {pi}{ital N}{Delta}} (various factors). {copyright} {ital 1995} {ital American} {ital Institute} {ital of} {ital Physics}.

  10. High-efficiency microstructured semiconductor neutron detectors for direct 3He replacement

    NASA Astrophysics Data System (ADS)

    Fronk, R. G.; Bellinger, S. L.; Henson, L. C.; Huddleston, D. E.; Ochs, T. R.; Sobering, T. J.; McGregor, D. S.

    2015-04-01

    High-efficiency Microstructured Semiconductor Neutron Detectors (MSNDs) have been tiled and arranged in a cylindrical form factor in order to serve as a direct replacement to aging and increasingly expensive 3He gas-filled proportional neutron detectors. Two 6-in long by 2-in diameter cylinders were constructed and populated with MSNDs which were then directly compared to a 4 atm Reuter Stokes 3He detector of the same dimensions. The Generation 1 MSND-based 3Helium-Replacement (HeRep Mk I) device contained sixty-four 1-cm2 active-area MSNDs, each with an intrinsic neutron detection efficiency of approximately 7%. A Generation 2 device (the HeRep Mk II) was populated with thirty 4-cm2 active-area MSNDs, with an intrinsic thermal neutron detection efficiency of approximately 30%. The MSNDs of each HeRep were integrated to count as a single device. The 3He proportional counter and the HeRep devices were tested while encased in a cylinder of high-density polyethylene measuring a total of 6-in by 9-in. The 3He counter and the HeRep Mk II were each placed 1 m from a 54-ng 252Cf source and tested for efficiency. The 3He proportional counter had a net count rate of 17.13±0.10 cps at 1 m. The HeRep Mk II device had a net count rate of 17.60±0.10 cps, amounting to 102.71±2.65% of the 3He gas counter while inside of the moderator. Outside of moderator, the 3He tube had a count rate of 3.35±0.05 cps and the HeRep Mk II device reported 3.19±05, amounting to 95.15±9.04% of the 3He neutron detector.

  11. A compact SEOP 3He neutron spin filter with AFP NMR

    NASA Astrophysics Data System (ADS)

    Ino, Takashi; Arimoto, Yasushi; Shimizu, Hirohiko M.; Sakaguchi, Yoshifumi; Sakai, Kenji; Kira, Hiroshi; Shinohara, Takenao; Oku, Takayuki; Suzuki, Jun-ichi; Kakurai, Kazuhisa; Chang, Lieh-Jeng

    2012-02-01

    We developed AFP NMR in an aluminum container for polarized noble gas nuclei. The radio frequency magnetic field inside the aluminum container was designed from computer simulations. The polarization loss by the AFP spin flip of 3He was measured to be as low as 3.8×10-4. With this technique, a compact in-situ polarizing 3He neutron spin filter with AFP NMR is demonstrated.

  12. Hyperpolarized (3) He and (129) Xe MRI: differences in asthma before bronchodilation.

    PubMed

    Svenningsen, Sarah; Kirby, Miranda; Starr, Danielle; Leary, Del; Wheatley, Andrew; Maksym, Geoffrey N; McCormack, David G; Parraga, Grace

    2013-12-01

    To compare hyperpolarized helium-3 ((3) He) and xenon-129 ((129) Xe) MRI in asthmatics before and after salbutamol inhalation. Seven asthmatics provided written informed consent and underwent spirometry, plethysmography, and MRI before and after salbutamol inhalation. (3) He and (129) Xe ventilation defect percent (VDP) and ventilation coefficient of variation (COV) were measured. To characterize the airways spatially related to ventilation defects, wall area percent (WA%) and lumen area (LA) were evaluated for two subjects who had thoracic x-ray computed tomography (CT) acquired 1 year before MRI. Before salbutamol inhalation, (129) Xe VDP (8 ± 5%) was significantly greater than (3) He VDP (6 ± 5%, P = 0.003). Post-salbutamol, there was a significant improvement in both (129) Xe (5 ± 4%, P < 0.0001) and (3) He (4 ± 3%, P = 0.001) VDP, and the improvement in (129) Xe VDP was significantly greater (P = 0.008). (129) Xe MRI COV (Pre: 0.309 ± 0.028, Post: 0.296 ± 0.036) was significantly greater than (3) He MRI COV (Pre: 0.282 ± 0.018, Post: 0.269 ± 0.024), pre- (P < 0.0001) and post-salbutamol (P < 0.0001) and the decrease in COV post-salbutamol was significant ((129) Xe, P = 0.002; (3) He, P < 0.0001). For a single asthmatic, a sub-segmental (129) Xe MRI ventilation defect that was visible only before salbutamol inhalation but not visible using (3) He MRI was spatially related to a remodeled fourth generation sub-segmental airway (WA% = 78%, LA = 2.9 mm(2) ). In asthma, hyperpolarized (129) Xe MRI may help reveal ventilation abnormalities before bronchodilation that are not observed using hyperpolarized (3) He MRI. Copyright © 2013 Wiley Periodicals, Inc.

  13. The Role of Serotonin Transporter in Human Lung Development and in Neonatal Lung Disorders

    PubMed Central

    Sen, P.; Parks, W. T.; Langston, C.

    2017-01-01

    Introduction. Failure of the vascular pulmonary remodeling at birth often manifests as pulmonary hypertension (PHT) and is associated with a variety of neonatal lung disorders including a uniformly fatal developmental disorder known as alveolar capillary dysplasia with misalignment of pulmonary veins (ACD/MPV). Serum serotonin regulation has been linked to pulmonary vascular function and disease, and serotonin transporter (SERT) is thought to be one of the key regulators in these processes. We sought to find evidence of a role that SERT plays in the neonatal respiratory adaptation process and in the pathomechanism of ACD/MPV. Methods. We used histology and immunohistochemistry to determine the timetable of SERT protein expression in normal human fetal and postnatal lungs and in cases of newborn and childhood PHT of varied etiology. In addition, we tested for a SERT gene promoter defect in ACD/MPV patients. Results. We found that SERT protein expression begins at 30 weeks of gestation, increases to term, and stays high postnatally. ACD/MPV patients had diminished SERT expression without SERT promoter alteration. Conclusion. We concluded that SERT/serotonin pathway is crucial in the process of pulmonary vascular remodeling/adaptation at birth and plays a key role in the pathobiology of ACD/MPV. PMID:28316463

  14. Radiation-enhanced Lung Cancer Progression in a Transgenic Mouse Model of Lung Cancer is Predictive of Outcomes in Human Lung and Breast Cancer

    PubMed Central

    Delgado, Oliver; Batten, Kimberly G.; Richardson, James A.; Xie, Xian-Jin; Gazdar, Adi F.; Kaisani, Aadil A.; Girard, Luc; Behrens, Carmen; Suraokar, Milind; Fasciani, Gail; Wright, Woodring E.; Story, Michael D.; Wistuba, Ignacio I.; Minna, John D.; Shay, Jerry W.

    2014-01-01

    Purpose Carcinogenesis is an adaptive process between nascent tumor cells and their microenvironment including the modification of inflammatory responses from anti-tumorigenic to pro-tumorigenic. Radiation exposure can stimulate inflammatory responses that inhibit or promote carcinogenesis. The purpose of this study is to determine the impact of radiation exposure on lung cancer progression in vivo and assess the relevance of this knowledge to human carcinogenesis. Experimental Design K-rasLA1 mice were irradiated with various doses and dose regimens and then monitored till death. Microarray analyses were performed using Illumina® BeadChips on whole lung tissue 70 days post-irradiation with a fractionated or acute dose of radiation and compared to age-matched unirradiated controls. Unique group classifiers were derived by comparative genomic analysis of three experimental cohorts. Survival analyses were performed using principal component analysis and k-means clustering on three lung adenocarcinoma, three breast adenocarcinoma, and two lung squamous carcinoma annotated microarray datasets. Results Radiation exposure accelerates lung cancer progression in the K-rasLA1 lung cancer mouse model with dose fractionation being more permissive for cancer progression. A non-random inflammatory signature associated with this progression was elicited from whole lung tissue containing only benign lesions and predicts human lung and breast cancer patient survival across multiple datasets. Immunohistochemical analyses suggest that tumor cells drive predictive signature. Conclusions These results demonstrate that radiation exposure can cooperate with benign lesions in a transgenic model of cancer by impacting inflammatory pathways, and that clinically relevant similarities exist between human lung and breast carcinogenesis. PMID:24486591

  15. Compressing Spin-Polarized 3He With a Modified Diaphragm Pump

    PubMed Central

    Gentile, T. R.; Rich, D. R.; Thompson, A. K.; Snow, W. M.; Jones, G. L.

    2001-01-01

    Nuclear spin-polarized 3He gas at pressures on the order of 100 kPa (1 bar) are required for several applications, such as neutron spin filters and magnetic resonance imaging. The metastability-exchange optical pumping (MEOP) method for polarizing 3He gas can rapidly produce highly polarized gas, but the best results are obtained at much lower pressure (~0.1 kPa). We describe a compact compression apparatus for polarized gas that is based on a modified commercial diaphragm pump. The gas is polarized by MEOP at a typical pressure of 0.25 kPa (2.5 mbar), and compressed into a storage cell at a typical pressure of 100 kPa. In the storage cell, we have obtained 20 % to 35 % 3He polarization using pure 3He gas and 35 % to 50 % 3He polarization using 3He-4He mixtures. By maintaining the storage cell at liquid nitrogen temperature during compression, the density has been increased by a factor of four. PMID:27500044

  16. Tritium/3He measurements in young groundwater: Progress in applications to complex hydrogeological systems

    USGS Publications Warehouse

    Schlosser, P.; Shapiro, S.D.; Stute, M.; Plummer, N.

    2000-01-01

    Tritium/3He dating has been applied to many problems in groundwater hydrology including, for example, determination of circulation patterns, mean residence times, recharge rates, or bank infiltration. Here, we discuss recent progress in the application of the tritium/3He dating method to sites with complex hydrogeological settings. Specifically, we report on tritium/3He dating at sites with (a) river infiltration into the basaltic fractured rock aquifer of the Eastern Snake River Plain, and (b) river infiltration through sinkholes into the karstic limestone Upper Floridian aquifer near Valdosta, Georgia.Tritium/3He dating has been applied to many problems in groundwater hydrology including, for example, determination of circulation patterns, mean residence times, recharge rates, or bank infiltration. Here, we discuss recent progress in the application of the tritium/3He dating method to sites with complex hydrogeological settings. Specifically, we report on tritium/3He dating at sites with (a) river infiltration into the basaltic fractured rock aquifer of the Eastern Snake River Plain, and (b) river infiltration through sinkholes into the karstic limestone Upper Floridian aquifer near Valdosta, Georgia.

  17. Primordial 3He in South Atlantic deep waters from sources on the Mid-Atlantic Ridge

    NASA Astrophysics Data System (ADS)

    Rüth, Christine; Well, Roland; Roether, Wolfgang

    2000-06-01

    Helium isotope data from three zonal WOCE sections (11°S, 19°S and 30°S) in the South Atlantic are presented. Among other features we find a distinct δ 3He-maximum above the Mid-Atlantic Ridge (MAR) at all three latitudes. Using a hydrographic multiparameter analysis, we separate 3He emanating from the MAR from the large-scale 3He background. To our knowledge, this is the first confirmation of input of primordial 3He at the MAR in the South Atlantic. The source appears to be weak compared with the Pacific sources, causing 3He elevations (relative to background values) of only 2-3% directly above the MAR. This exceeds by several times the statistical and systematic data uncertainties, which amount to 0.35% each, so that detailed contouring of the MAR-derived 3He is possible. At 30°S and 11°S, a significant signal extends westward over at least 2000 km, whereas at 19°S the signal is more confined to the ridge area. The westward extensions indicate westward flow at depths near the ridge crest elevation, contradicting flow directions deduced previously by Reid (1989).

  18. Fusion gamma-ray measurements for D-3He experiments at JT-60U

    NASA Astrophysics Data System (ADS)

    Nishitani, T.; Tobita, K.; Kusama, Y.; Shibata, Y.

    2001-01-01

    Fusion gamma rays were measured in D-3He experiments using negative ion-based neutral beam injection (N-NBI) in reverse shear plasmas of the JT-60 tokamak. 3He gas was puffed at plasma initiation and just before N-NB injection. The D-3He reaction produces 3.6 MeV alphas and 14.7 MeV protons, but there is also a small branch which provides 5Li and 16.7 MeV gamma rays. The total D-3He reaction rate can be evaluated from measurement of gamma rays of the 3He (d,γ) 5Li reactions using a 3 in. diam by 3 in. long Bi4Ge3O12 scintillator. The gamma-ray detector was located 17 m below the plasma center and measured the gamma-rays in a vertical line of sight. The detector was mounted inside a heavy collimator with polyethylene and lead shielding. The floor penetration, a 4×8 cm2 hole, was used as a precollimator. Energy calibration of the detector was done with photopeaks for neutron capture gamma rays from the structural materials in D-D discharges. The detection efficiency was calculated with Monte Carlo code MCNP-4B for 16.7 MeV gammas. The pulse height analysis of the gamma rays resulted in the D-3He fusion power of 110±30 kW in this experiment.

  19. Transport of polarized 3He for the nEDM experiment at the SNS

    NASA Astrophysics Data System (ADS)

    Rao, Thomas; Beck, Douglas; Koivuniemi, Jaakko; Silvera, Ike; Williamson, Steven; Yao, Weijun; nEDM Collaboration

    2016-09-01

    The neutron electric dipole moment (nEDM) experiment at the ORNL SNS aims to determine the neutron's electric dipole moment to an accuracy of 5.4 x 10-28 e cm by measuring the Lamor precession of neutrons using the spin dependent reaction n +3He =>p +3H +764KeV. In the experiment polarized 3He is injected into a free surface of 4He, and then brought to the measurement cell and removed once it depolarizes. The proposed transport method for the 3He, the heat flush mechanism, must be tested. In the heat flush mechanism a thermal gradient along a long pipe, generates phonons whose collisions with 3He, drives 3He transport to the cold end of the pipe. Tests of the heat flush mechanism by measuring the change in 3He concentration at the cold end of a long pipe, using a capacitive pressure sensor, are underway at Harvard University. Work supported in part by NSF Grants PHY-1440011 and PHY-1506416.

  20. An Update on 3He Correlation Function Research for the SNS nEDM collaboration

    NASA Astrophysics Data System (ADS)

    Reid, Austin; Golub, Robert; Dipert, Robert

    2016-09-01

    In the 65 years since Ramsey's null result for the neutron's permanent electric dipole moment (nEDM), techniques have become increasingly sensitive, establishing the present upper limit of 3 ×10-26 e .cm . This value was limited by an unexpected source of error: a freqency shift with linear dependence on the electric field colloquially called a false EDM. The next generation nEDM sensing apparatus being developed for the Spallation Neutron Source at Oak Ridge National Laboratory uses a 3He comagenetometer in a pure helium-II bath. The false EDM in 3He may be related to the 3He's position autocorrelation function, which in turn is accessible by a detailed study of T1 decay in hyperpolarized 3He. Existing measurements of this system were limited by temperature, noise, and 3He concentration. Dramatic improvements have been made on all three fronts by improving the thermal connection between the measurment cell and the dilution refrigerator, by adding additional shielding and a SQUID package, and by developing a MEOP 3He polarization system. Data collection is underway. This material is based upon work supported by the U.S. Department of Energy, Office of Science, Office of Nuclear Physics, under Award Number DE-FG02-97ER41042.

  1. Growth suppressive efficacy of human lak cells against human lung-cancer implanted into scid mice.

    PubMed

    Teraoka, S; Kyoizumi, S; Suzuki, T; Yamakido, M; Akiyama, M

    1995-06-01

    The purpose of our study was to determine the efficacy of immunotherapy using human lymphokine activated killer (LAK) cells against a human-lung squamous-cell carcinoma cell line (RERF-LC-AI) implanted into severe combined immunodeficient (SCID) mice. A statistically significant growth suppressive effect on RERF-LC-AI implanted into SCID mice was observed when human LAK cells were administered into the caudal vein of the mice treated with a continuous supply (initiated prior to LAK cells injection) of rIL-2. The human LAK cells stained with PKH 2, a fluorescent dye, for later detection using flow cytometry were administered into the caudal vein of RERF-LC-AI bearing SCID mice; the cells persisted for 7 days in the implanted lung cancer tissue and in the mouse peripheral blood, but for 5 days in the mouse spleen. The number of infiltrated human LAK cells in each tissue increased dose-dependently with the number of injected cells. The results indicate that the antitumor effect most likely occurred during the early implantation period of the human LAK cells. These results demonstrate the applicability of this model to the in vivo study of human lung cancer therapy.

  2. Terrestrial cosmogenic 3He: where are we 30 years after its discovery?

    NASA Astrophysics Data System (ADS)

    Blard, Pierre-Henri; Pik, Raphaël; Farley, Kenneth A.; Lavé, Jérôme; Marrocchi, Yves

    2016-04-01

    It is now 30 years since cosmogenic 3He has been detected for the first time in a terrestrial sample (Kurz, 1986). 3He is now a widely used geochemical tool in many fields of Earth sciences: volcanology, tectonics, paleoclimatology. 3He has the advantage to have a high "production rate" to "detection limit" ratio, allowing surfaces as young as hundred of years to be dated. Although its nuclear stability implies several limitations, it moreover represents a useful alternative to 10Be in mafic environments. This contribution is a review of the progresses that have been accomplished since this discovery, and discuss strategies to improve both the accuracy and the precision of this geochronometer. 1) Measurement of cosmogenic 3He Correction of magmatic 3He. To estimate the non-cosmogenic magmatic 3He, Kurz (1986) invented a two steps method involving crushing of phenocrysts (to analyze the isotopic ratio of the magmatic component), followed by a subsequent melting of the sample, to extract the remaining components, including the cosmogenic 3He: 3Hec = 3Hemelt -4Hemelt x (3He/4He)magmatic (1) Several studies suggested that the preliminary crushing may induce a loss of cosmogenic 3He (Hilton et al., 1993; Yokochi et al., 2005; Blard et al., 2006), implying an underestimate of the cosmogenic 3He measurement. However, subsequent work did not replicate these observations (Blard et al., 2008; Goerhing et al., 2010), suggesting an influence of the used apparatus. An isochron method (by directly melting several phenocrysts aliquots) is an alternative to avoid the preliminary crushing step (Blard and Pik, 2008). Atmospheric contamination. Protin et al. (in press) provides robust evidences for a large and irreversible contamination of atmospheric helium on silicate surfaces. This unexpected behavior may reconcile the contrasted observations about the amplitude of crushing loss. This undesirable atmospheric contamination is negligible if grain fractions smaller than 150 mm are

  3. Evaluation of a mouse model of necrotic granuloma formation using C3HeB/FeJ mice for testing of drugs against Mycobacterium tuberculosis.

    PubMed

    Driver, Emily R; Ryan, Gavin J; Hoff, Donald R; Irwin, Scott M; Basaraba, Randall J; Kramnik, Igor; Lenaerts, Anne J

    2012-06-01

    Persistence of Mycobacterium tuberculosis remains a significant challenge for the effective treatment of tuberculosis in humans. In animals that develop necrotic lung lesions following infection with M. tuberculosis, drug-tolerant bacilli are present and persist in an extracellular microenvironment within the necrotic cores. In this study, we examined the efficacy of drug treatment in C3HeB/FeJ (Kramnik) mice that develop lesions with liquefactive necrosis, in comparison to BALB/c mice that develop nonnecrotic lesions following aerosol challenge. To accomplish this, Kramnik and BALB/c mice were infected by aerosol with M. tuberculosis and treated for 7 to 8 weeks with monotherapy using drugs with different modes of action. The efficacy of drug therapy was quantified by enumeration of bacterial load. The progression of disease and location and distribution of bacilli within lesions were visualized using various staining techniques. In the late stages of infection, Kramnik mice developed fibrous encapsulated lung lesions with central liquefactive necrosis containing abundant extracellular bacilli, whereas BALB/c mice formed nonnecrotic lesions with primarily intracellular bacilli. Necrotic lesions in Kramnik mice showed evidence of hypoxia by pimonidazole staining. Kramnik mice were significantly more refractory to drug therapy, especially for pyrazinamide. Metronidazole showed no bactericidal activity in either model. There were significantly higher numbers of drug-resistant colonies isolated from the Kramnik mice compared to BALB/c mice. These results suggest that the Kramnik mouse model will be a valuable model to test antituberculosis drugs, especially against bacilli that persist within necrotic lesions.

  4. The cytotoxicity and genotoxicity of soluble and particulate cobalt in human lung epithelial cells.

    PubMed

    Xie, Hong; Smith, Leah J; Holmes, Amie L; Zheng, Tongzhang; Pierce Wise, John

    2016-05-01

    Cobalt is a toxic metal used in various industrial applications leading to adverse lung effects by inhalation. Cobalt is considered a possible human carcinogen with the lung being a primary target. However, few studies have considered cobalt-induced toxicity in human lung cells, especially normal lung epithelial cells. Therefore, in this study, we sought to determine the cytotoxicity and genotoxicity of particulate and soluble cobalt in normal primary human lung epithelial cells. Cobalt oxide and cobalt chloride were used as representative particulate and soluble cobalt compounds, respectively. Exposure to both particulate and soluble cobalt induced a concentration-dependent increase in cytotoxicity, genotoxicity, and intracellular cobalt ion levels. Based on intracellular cobalt ion levels, we found that soluble and particulate cobalt induced similar cytotoxicity while soluble cobalt was more genotoxic than particulate cobalt. These data indicate that cobalt compounds are cytotoxic and genotoxic to human lung epithelial cells. © 2016 Wiley Periodicals, Inc.

  5. An important source of 4He (and 3He) in diamonds

    NASA Astrophysics Data System (ADS)

    Lal, D.

    1989-12-01

    A large data base has recently accumulated on the concentrations of helium isotopes in diamonds mined from various regions. It was noted earlier (Ozima et al. (1985) [1]; Lal et al. (1989) [2]) that the frequency distribution of the 4He concentrations is a fairly narrow one, whereas that of 3He concentrations is a broad one with no pronounced peaks. The ratios 3He/ 4He , on the other hand show a broad maximum around 2 R a ( R a equals atmospheric 3He/ 4He ratio, = 1.40 × 10 -6) with a slow decrease over two orders of magnitude on either side. Does this imply that the diamonds sample a wide variety of helium reservoirs having a range of 3He/ 4He ratios but somehow attain similar 4He concentrations? We propose that in a majority of the diamonds studied, 4He is primarily due to implantation of radiogenic alpha particles from the host material after emplacement in the crust, usually kimberlite, and that the concentrations of 4He in diamonds often get appreciably altered by this process. Thus the 4He trapped in the diamond at the time of its crystallization is usually overwhelmed by the implanted helium and the measured 3He/ 4He ratios do not generally correspond to any "sources" in the mantle. However, the implanted 4He resides in the outer 16 μm of the diamond, and the intrinsic 4He and 3He/ 4He ratios in the diamond can be studied if its outer layers are removed. The wider implications of diamond being the "target" material for nuclear reaction products from the host material are discussed. Radiogenic 3He produced in the host material is also implanted in the diamond, but this contribution is small on a gross basis. However, since the depth of implantation of 3He is greater than that of 4He, some of the very high 3He/ 4He ratios observed in diamonds could be due to the "implantation" of radiogenic 3He. The radiogenic reactions in the host material can also contribute to appreciable 21Ne in diamonds.

  6. Cross section of 3He(3He,2p)4He measured over the range of 45 to 26 keV

    NASA Astrophysics Data System (ADS)

    Itahashi, T.; Kudomi, N.; Yoshida, S.; Komori, M.; Takahisa, K.; Ejiri, H.; Toki, H.; Nagai, Y.; Ohsumi, H.

    2002-04-01

    We have measured the nuclear fusion cross section for 3He(3He,2p)4He near the solar Gamow peak with a compact accelerator facility OCEAN and with an assembly of counter telescopes with a detection efficiency of 10%. The data obtained at Ecm=45.3 to 31.2 keV improved the existing astrophysical S-factors in statistical and systematic errors. A Monte Carlo simulation program exploiting GEANTS, SRIM and GENBOD computer codes has been developed to estimate the detection efficiency for two proton coincidence with a ΔE-E telescopes. The precision of developed program has been investigated by comparing the simulated results with the experimental values for cross section of the D(3He,p)α reaction carried out by replacing the target with D gas. It turned out that the present study involves around 3% systematic errors for an estimation of the detection efficiency. The overall systematic errors for these data from 45.3 to 31.2 keV is 3.8%, that is as good as those of the previous experiments. The latest data obtained at 29.1 and 26.9 keV with large statistical and systematic errors show considerable larger S-factors than previous. An enhancement of astrophysical S-factor might be expected by the existence of a certain resonance as well as by an atomic effect on the screening potential near the solar Gamow energy. .

  7. Cross Section of 3HE(3HE,2P)4HE Measured Over the Energy Range of 45 TO 26 KEV

    NASA Astrophysics Data System (ADS)

    Itahashi, T.; Kudomi, N.; Yoshida, S.; Komori, M.; Takahisa, K.; Ejiri, H.; Toki, H.; Nagai, Y.; Ohsumi, H.

    We have measured the nuclear fusion cross section for 3HE(3HE,2P)4HE near the solar Gamow peak with a compact accelerator facility OCEAN and with an assembly of counter telescopes with a detection efficiency of 10%. The data obtained at Ecm= 45.3, 43.3, 41.3, 39.3, 37.3, 35.2, 33.1, 31.2 keV improved the existing astro-physical S-factors in statistical and systematic errors. A Monte Carlo simulation program exploiting GEANT3, SRJM and GENBOD computer codes has been developed to estimate the detection efficiency for two proton coincidence with a ΔE-E counter telescopes. The precision of developed program has been investigated by comparing the simulated results with the experimental values for cross section of the D(3He, p)α reaction carried out by replacing the target with deuterium gas. It turned out that the present study involves around 3% systematic errors for an estimation of the detection efficiency. The overall systematic errors for these data from 45.3 to 31.2 keV is 3.8%, that is as good as those of the previous experiments.

  8. Minority and mode conversion heating in (3He)-H JET plasmas

    NASA Astrophysics Data System (ADS)

    Van Eester, D.; Lerche, E.; Johnson, T. J.; Hellsten, T.; Ongena, J.; Mayoral, M.-L.; Frigione, D.; Sozzi, C.; Calabro, G.; Lennholm, M.; Beaumont, P.; Blackman, T.; Brennan, D.; Brett, A.; Cecconello, M.; Coffey, I.; Coyne, A.; Crombe, K.; Czarnecka, A.; Felton, R.; Gatu Johnson, M.; Giroud, C.; Gorini, G.; Hellesen, C.; Jacquet, P.; Kazakov, Ye; Kiptily, V.; Knipe, S.; Krasilnikov, A.; Lin, Y.; Maslov, M.; Monakhov, I.; Noble, C.; Nocente, M.; Pangioni, L.; Proverbio, I.; Stamp, M.; Studholme, W.; Tardocchi, M.; Versloot, T. W.; Vdovin, V.; Whitehurst, A.; Wooldridge, E.; Zoita, V.; EFDA Contributors, JET

    2012-07-01

    Radio frequency (RF) heating experiments have recently been conducted in JET (3He)-H plasmas. This type of plasmas will be used in ITER's non-activated operation phase. Whereas a companion paper in this same PPCF issue will discuss the RF heating scenario's at half the nominal magnetic field, this paper documents the heating performance in (3He)-H plasmas at full field, with fundamental cyclotron heating of 3He as the only possible ion heating scheme in view of the foreseen ITER antenna frequency bandwidth. Dominant electron heating with global heating efficiencies between 30% and 70% depending on the 3He concentration were observed and mode conversion (MC) heating proved to be as efficient as 3He minority heating. The unwanted presence of both 4He and D in the discharges gave rise to 2 MC layers rather than a single one. This together with the fact that the location of the high-field side fast wave (FW) cutoff is a sensitive function of the parallel wave number and that one of the locations of the wave confluences critically depends on the 3He concentration made the interpretation of the results, although more complex, very interesting: three regimes could be distinguished as a function of X[3He]: (i) a regime at low concentration (X[3He] < 1.8%) at which ion cyclotron resonance frequency (ICRF) heating is efficient, (ii) a regime at intermediate concentrations (1.8 < X[3He] < 5%) in which the RF performance is degrading and ultimately becoming very poor, and finally (iii) a good heating regime at 3He concentrations beyond 6%. In this latter regime, the heating efficiency did not critically depend on the actual concentration while at lower concentrations (X[3He] < 4%) a bigger excursion in heating efficiency is observed and the estimates differ somewhat from shot to shot, also depending on whether local or global signals are chosen for the analysis. The different dynamics at the various concentrations can be traced back to the presence of 2 MC layers and their

  9. Red cell pulmonary transit times through the healthy human lung.

    PubMed

    Zavorsky, G S; Walley, K R; Russell, J A

    2003-03-01

    It has previously been postulated that rapid red cell capillary transit through the human lung plays a role in the mechanism of diffusion limitation in some endurance athletes. Methodological limitations currently prevent researchers from directly measuring pulmonary capillary transit times in humans during exercise; however, first pass radionuclide cardiography allows direct measurement of red blood cell (RBC) transit times through the whole lung at various exercise intensities. We examined the relationship between mean whole lung red cell pulmonary transit times (cardiopulmonary transit times or CPTT) and different levels of flow in 88 healthy humans (76 males, 12 females) from several studies (mean age 31 years). The pooled data suggest that the relationship between CPTT and cardiac index (CI), beginning at rest and progressing through to maximum exercise demonstrates that CPTT reaches its minimum value when CI is about 8.1 l m2 x min(-1) (2.5-3 times the CI value at rest), and does not significantly change with further increases in CI. Cardiopulmonary blood volume (CPBV) index also does not change significantly until CI reaches 2.5 to 3 times the CI value at rest and then increases roughly linearly after that point. Consequently, the systematic increase in CPBV index with increasing pulmonary blood flow between 8.1 and 20 l m2 x min(-1) displays an adaptive response of the cardiopulmonary system by augmenting CPBV (and perhaps pulmonary capillary blood volume through distension and recruitment) to offset the reduction in CPTT, as no significant difference in mean CPTT is observed between these levels of flow (P > 0.05). Therefore, these data demonstrate that CPBV does not reach maximum capacity during strenuous or maximum exercise. This does not support the principle of quarter-power allometric scaling for flow when explaining modifications during exercise. Therefore, we speculate that the observed relationships between CPTT, CBPV index and flow may prevent

  10. Establishment of a human lung cancer cell line with high metastatic potential to multiple organs: gene expression associated with metastatic potential in human lung cancer.

    PubMed

    Nakano, Tetsuhiro; Shimizu, Kimihiro; Kawashima, Osamu; Kamiyoshihara, Mitsuhiro; Kakegawa, Seiichi; Sugano, Masayuki; Ibe, Takashi; Nagashima, Toshiteru; Kaira, Kyoichi; Sunaga, Noriaki; Ohtaki, Youichi; Atsumi, Jun; Takeyoshi, Izumi

    2012-11-01

    Convenient and reliable multiple organ metastasis model systems might contribute to understanding the mechanism(s) of metastasis of lung cancer, which may lead to overcoming metastasis and improvement in the treatment outcome of lung cancer. We isolated a highly metastatic subline, PC14HM, from the human pulmonary adenocarcinoma cell line, PC14, using an in vivo selection method. The expression of 34,580 genes was compared between PC14HM and parental PC14 by cDNA microarray analysis. Among the differentially expressed genes, expression of four genes in human lung cancer tissues and adjacent normal lung tissues were compared using real-time reverse transcription polymerase chain reaction. Although BALB/c nude mice inoculated with parental PC14 cells had few metastases, almost all mice inoculated with PC14HM cells developed metastases in multiple organs, including the lung, bone and adrenal gland, the same progression seen in human lung cancer. cDNA microarray analysis revealed that 981 genes were differentially (more than 3-fold) expressed between the two cell lines. Functional classification revealed that many of those genes were associated with cell growth, cell communication, development and transcription. Expression of three upregulated genes (HRB-2, HS3ST3A1 and RAB7) was higher in human cancer tissue compared to normal lung tissue, while expression of EDG1, which was downregulated, was lower in the cancer tissue compared to the normal lung. These results suggest that the newly established PC14HM cell line may provide a mouse model of widespread metastasis of lung cancer. This model system may provide insights into the key genetic determinants of widespread metastasis of lung cancer.

  11. Photodisintegration of /sup 3/H and /sup 3/He. [Threshold to 25 MeV

    SciTech Connect

    Faul, D.D.

    1980-09-01

    The photoneutron cross sections for /sup 3/H and /sup 3/He have been measured from threshold to approx. 25 MeV with monoenergetic photons from the annihilation in flight of fast positrons at the LLL Electron-Positron Linear Accelerator facility. These reactions include the two-body breakup of /sup 3/H and the three-body breakup of both /sup 3/H and /sup 3/He; these measurements for /sup 3/H are the first to span the energy region across the peaks of the cross sections. An efficient BF/sub 3/-tube-and-paraffin neutron detector and high-pressure gaseous samples of several moles each (the activity of the /sup 3/H sample was approx. 200,000 Ci) were employed in these measurements. Measurements on /sup 16/O and /sup 2/H also were performed to verify the absolute cross-section scale. The results, when compared with each other and with results for the two-body breakup cross section for /sup 3/He from the literature, show that the two-body breakup cross sections for /sup 3/H and /sup 3/He have nearly the same shape, but the one for /sup 3/He lies lower in magnitude; the three-body breakup cross section for /sup 3/He lies higher in magnitude and is broader in the peak region and also rises less sharply from threshold than that for /sup 3/H; and these measured differences between the cross sections for the breakup modes largely compensate in their sum, so that the total photon absorption cross sections for /sup 3/H and /sup 3/He are nearly the same in both size and shape at energies near and above their peaks. Theoretical results from the literature disagree with the experimental results to a certain extent over the entire photon-energy region for which the photoneutron cross sections were measured. 50 figures, 7 tables.

  12. Grand Comore Island: A well-constrained “low 3He/4He” mantle plume

    NASA Astrophysics Data System (ADS)

    Class, Cornelia; Goldstein, Steven L.; Stute, Martin; Kurz, Mark D.; Schlosser, Peter

    2005-05-01

    We report He isotope ( 3He/ 4He) variations in samples from alkali basaltic and basanitic lava flows from Grande Comore Island complemented by existing [1,2] [C. Class, S.L. Goldstein, Plume-lithosphere interactions in the ocean basins: constraints from the source mineralogy. Earth Planet. Sci. Lett., 150 (1997) 245-260, C. Class, S.L. Goldstein, R. Altherr, P. Bachèchlery, The process of plume-lithosphere interaction in the ocean basins—the case of Grande Comore. J. Petrol., 39 (5) (1998) 881-903] and new Sr-Nd-Pb isotope ratios and major and trace element abundances. He isotope data in samples from Tristan da Cunha and Gough islands and the Huri Hills in Kenya are reported also. Grande Comore 3He/ 4He ratios vary between 5.05 and 7.08 RA ( 4He/ 3He ≈ 141,000-101,000). Chemical and Sr-Nd-Pb isotopic variations of Grande Comore lavas were previously shown to reflect melts derived from the deep mantle plume and the shallow lithospheric mantle [1-3] [C. Class, S.L. Goldstein, Plume-lithosphere interactions in the ocean basins: constraints from the source mineralogy. Earth Planet. Sci. Lett., 150 (1997) 245-260, C. Class, S.L. Goldstein, R. Altherr, P. Bachèchlery, The process of plume-lithosphere interaction in the ocean basins-the case of Grande Comore. J. Petrol., 39 (5) (1998) 881-903, C. Claude-Ivanaj, B. Bourdon, C.J. Allègre, Ra-Th-Sr isotope systematics in Grande Comore Island: a case study of plume-lithosphere interaction. Earth Planet. Sci. Lett. 164 (1998) 99-117]. The lithosphere-dominated end-member (La Grille volcano) shows uniform 3He/ 4He ratios within error of 6.75-7.08 RA ( 4He/ 3He ≈ 106,000-101,000) over a range of [He] = 36-428 × 10 - 9 ccSTP/g. The plume end-member (of the Karthala volcano suite), as constrained by Sr, Nd, Pb isotope ratios, shows uniformly lower 3He/ 4He ratios with 5.05-5.41 RA ( 4He/ 3He ≈ 141,000-132,000) over a range of [He] = 11-136 × 10 - 9 ccSTP/g. All samples show good correlations between Sr-Nd-He isotope

  13. Titanium Isotopes Link the High 3He/4He Reservoir to Continent Formation

    NASA Astrophysics Data System (ADS)

    Millet, M. A.; Jackson, M. G.; Dauphas, N.; Burton, K. W.; Williams, H. M.; Kurz, M. D.; Doucelance, R.; Smithies, H.; Champion, D. C.; Nowell, G. M.

    2016-12-01

    Elevated 3He/4He ratios found in ocean island basalts (OIB) argue for the survival of an early-formed reservoir (>4.5 Ga) in the Earth's mantle [1]. However, its nature remains debated. A characteristic of high 3He/4He OIBs is their anomalous enrichment in Ti abundance relative to elements of similar incompatibility (Sm and Tb). Here we use a new geochemical tool, the stable isotopes of Titanium, to investigate the origin of Ti enrichment of high 3He/4He OIBs. Recent work [2] has shown that Ti isotopes are a powerful tracer of oxide-melt equilibrium in magmatic systems. Results show that primitive OIB samples from localities associated with low 3He/4He ratios (≤15 R/Ra) have δ49Ti values within error of the mantle (δ49Ti=0.005±0.005 [2]) and chondrite values (+0.004±0.010 [3]) regardless of their Ti anomaly (0.93He/4He ratios (>25R/Ra) display δ49Ti values ranging from mantle-like to enriched in light isotopes (up to -0.065‰±0.005) that are negatively correlated with their Ti/Ti* and uncorrelated to indices of magma differentiation. This indicates that i) elevated Ti/Ti* in high 3He/4He OIBs is a mantle source signature and ii) that the high 3He/4He reservoir is enriched in light isotopes of Ti relative to the BSE. This enrichment in light isotopes is balanced by the heavy δ49Ti values and negative Ti/Ti* of Archean Tonalite-Throndhjemite-Granodiorite samples (TTG) from the Pilbara and Yilgarn Craton (+0.20<δ49Ti<+0.40), a proxy for early-formed, juvenile continental crust. Given the chondritic composition of the Earth's mantle and the inability of the typical mantle lithologies to fractionate Ti isotopes, this implies that the Ti enrichment of the high 3He/4He mantle reservoir is linked to the recycling of residues of partial melting events in the presence of rutile (TiO2), a process that drove continent formation in the Archean and possibly earlier. In addition, since these residues cannot display elevated 3He/4He ratios, it requires the Ti

  14. Evaluation of a New Ultrasound Thoracoscope for Localization of Lung Nodules in Ex Vivo Human Lungs.

    PubMed

    Ujiie, Hideki; Kato, Tatsuya; Hu, Hsin-Pei; Hasan, Suhaib; Patel, Priya; Wada, Hironobu; Lee, Daiyoon; Fujino, Kosuke; Hwang, David M; Cypel, Marcelo; de Perrot, Marc; Pierre, Andrew; Darling, Gail; Waddell, Thomas K; Keshavjee, Shaf; Yasufuku, Kazuhiro

    2017-03-01

    Localization of small, nonvisible and nonpalpable nodules is challenging during video-assisted thoracoscopic surgery. We evaluated the feasibility of using a new ultrasound thoracoscope to localize nodules in resected ex vivo human lungs. The tumor was localized and measured in its greatest dimension with a prototype ultrasound thoracoscope (XLTF-UC180; Olympus Corporation, Tokyo, Japan) at different frequencies (5.0 to 12.0 MHz) and different lung specimen states (deflated, semiinflated). Measured tumor size and depth from lung surface were compared and correlated to the true diameter and depth from lung surface acquired from pathologic morphology. Ex vivo evaluation was performed on 16 solid nodules and nine part solid ground-glass nodules. All tumors were successfully localized in the deflated lung specimens (average size, 13.7 ± 5.2 mm). The tumor boundaries were best evaluated with an ultrasound frequency of 10 MHz. Solid nodules were more easily visualized than ground-glass nodules. Part solid ground-glass nodules were not easily detected in the semiinflated specimen owing to peritumoral air surrounding the tumor. Tumor boundaries were also difficult to identify in deeply situated tumors and in lungs with underlying disease. A strong positive correlation existed between the ultrasound measurement and true measurement of tumor size (R(2) = 0.89, p < 0.001). The ultrasound thoracoscope can be used to localize nodules in resected human lungs. The clarity of the tumor boundaries is influenced by the tumor type and depth and the underlying pulmonary disease. Complete lung deflation and the use of 10 MHz ultrasound frequency optimize the visualization of target tumors. Copyright © 2017 The Society of Thoracic Surgeons. Published by Elsevier Inc. All rights reserved.

  15. Preferential elevation of Prx I and Trx expression in lung cancer cells following hypoxia and in human lung cancer tissues.

    PubMed

    Kim, H J; Chae, H Z; Kim, Y J; Kim, Y H; Hwangs, T S; Park, E M; Park, Y M

    2003-10-01

    Transient/chronic microenvironmental hypoxia that exists within a majority of solid tumors has been suggested to have a profound influence on tumor growth and therapeutic outcome. Since the functions of novel antioxidant proteins, peroxiredoxin I (Prx I) and II, have been implicated in regulating cell proliferation, differentiation, and apoptosis, it was of our special interest to probe a possible role of Prx I and II in the context of hypoxic tumor microenvironment. Since both Prx I and II use thioredoxin (Trx) as an electron donor and Trx is a substrate for thioredoxin reductase (TrxR), we investigated the regulation of Trx and TrxR as well as Prx expression following hypoxia. Here we show a dynamic change of glutathione homeostasis in lung cancer A549 cells and an up-regulation of Prx I and Trx following hypoxia. Western blot analysis of 10 human lung cancer and paired normal lung tissues also revealed an elevated expression of Prx I and Trx proteins in lung cancer tissues. Immunohistochemical analysis of the lung cancer tissues confirmed an augmented Prx I and Trx expression in cancer cells with respect to the parenchymal cells in adjacent normal lung tissue. Based on these results, we suggest that the redox changes in lung tumor microenvironment could have acted as a trigger for the up-regulation of Prx I and Trx in lung cancer cells. Although the clinical significance of our finding awaits more rigorous future study, preferential augmentation of the Prx I and Trx in lung cancer cells may well represent an attempt of cancer cells to manipulate a dynamic redox change in tumor microenvironment in a manner that is beneficial for their proliferation and malignant progression.

  16. Comparison of human lung tissue mass measurements from ex vivo lungs and high resolution CT software analysis.

    PubMed

    Henne, Erik; Anderson, Joseph C; Lowe, Norma; Kesten, Steven

    2012-05-14

    Quantification of lung tissue via analysis of computed tomography (CT) scans is increasingly common for monitoring disease progression and for planning of therapeutic interventions. The current study evaluates the quantification of human lung tissue mass by software analysis of a CT to physical tissue mass measurements. Twenty-two ex vivo lungs were scanned by CT and analyzed by commercially available software. The lungs were then dissected into lobes and sublobar segments and weighed. Because sublobar boundaries are not visually apparent, a novel technique of defining sublobar segments in ex vivo tissue was developed. The tissue masses were then compared to measurements by the software analysis. Both emphysematous (n = 14) and non-emphysematous (n = 8) bilateral lungs were evaluated. Masses (Mean ± SD) as measured by dissection were 651 ± 171 g for en bloc lungs, 126 ± 60 g for lobar segments, and 46 ± 23 g for sublobar segments. Masses as measured by software analysis were 598 ± 159 g for en bloc lungs, 120 ± 58 g for lobar segments, and 45 ± 23 g for sublobar segments. Correlations between measurement methods was above 0.9 for each segmentation level. The Bland-Altman analysis found limits of agreement at the lung, lobe and sublobar levels to be -13.11% to -4.22%, -13.59% to 4.24%, and -45.85% to 44.56%. The degree of concordance between the software mass quantification to physical mass measurements provides substantial evidence that the software method represents an appropriate non-invasive means to determine lung tissue mass.

  17. 3He spin filter based polarized neutron capability at the NIST Center for Neutron Research

    NASA Astrophysics Data System (ADS)

    Chen, W. C.; Gentile, T. R.; Erwin, R.; Watson, S.; Ye, Q.; Krycka, K. L.; Maranville, B. B.

    2014-07-01

    A 3He neutron spin filter (NSF) program for polarized neutron scattering was launched in 2006 as part of the National Institute of Standards and Technology (NIST) Center for Neutron Research (NCNR) Expansion Initiative. The goal of the project was to enhance the NCNR polarized neutron measurement capabilities. Benefitting from more than a decade's development of spin-exchange optical pumping (SEOP) at NIST, we planned to employ SEOP based 3He neutron spin filters for the polarized neutron scattering community. These 3He NSF devices were planned for use on different classes of polarized neutron instrumentation at the NCNR, including triple-axis spectrometers (TAS), small-angle neutron scattering instruments (SANS), reflectometers, and wide-angle polarization analysis. Among them, the BT-7 thermal TAS, NG-3 SANS, and MAGIK reflectometer have already been in the user program for routine polarized beam experiments. Wide-angle polarization analysis on Multi-Axis Crystal Spectrometer (MACS) has been developed for user experiments. We describe briefly the SEOP systems dedicated for polarized beam experiments and polarizing neutron development for each instrument class. We summarize the current status and polarized neutronic performance for each instrument. We present a 3He NSF hardware and software interface to allow for synchronization of 3He polarization inversion (neutron spin flipping) and free-induction decay (FID) nuclear magnetic resonance (NMR) measurements with neutron data collection.

  18. ^3He Polarization by Rb Spin Exchange in a Multistage System

    NASA Astrophysics Data System (ADS)

    Coulter, K. P.; Chupp, T. E.; Smith, T. B.; Welsh, R. C.; Zerger, J. N.

    1999-10-01

    Polarization of ^3He by spin exchange with optically pumped Rb has benefited greatly from the use of high powered laser diode arrays. Efficient use of these lasers requires operation of cells with high ^3He densities to match better the pressure broadened Rb absorption line to the wide laser spectral profile. However, lower delivery pressures are often required. For example, for low energy neutron spin filters the optimum ^3He thickness (for practical polarizations) would produce impractically thin cells. A multistage system is practical for applications requiring high ^3He polarization delivered at variable pressure because the optical pumping stage can be separated from the delivery/refilling stages. Additionally, operation can be improved by choosing the appropriate glass for each stage. We have constructed a multistage system that consists of a 70 cc pump cell (Corning 7056 glass), a transition region (Pyrex Glass), and a 350 cc receiving cell (Cs-coated Fused Silica). The cells are connected using commercial Viton-rubber o-ring sealed Pyrex glass valves and ball and socket joints. The transition region is connected to a vacuum pump and gas fill system so that cells may be refilled in situ. Both pump cells and receiving cells have exhibited intrinsic ^3He relaxation times of >35 hours. We will report on tests of this prototype system.

  19. Longitudinal and transverse spin diffusion in3He-4He solutions in a strong magnetic field

    NASA Astrophysics Data System (ADS)

    Ager, J. H.; Child, A.; König, R.; Owers-Bradley, J. R.; Bowley, R. M.

    1995-06-01

    Using pulsed NMR techniques, we have measured spin diffusion in3He-3He solutions with3He concentrations of 0.05%, 0.1%, 0.46%, 1.0%, 3.8% and 6.4% in a magnetic field of 8.8 Tesla for a temperature range 11 mK⩽ T ⩽ 200 mK. We observe that the temperature dependence of the transverse spin diffusion coefficient D1 deviates from that expected for an unpolarized Fermi liquid in the degenerate region in the 1.0%, 3.8% and 6.4% solutions. Moreover, by measuring both longitudinal and transverse spin diffusion coefficients in the 6.4%-mixture, we have verified experimentally the difference between them, and provided direct evidence for a field-induced anisotropy in spin diffusion. The results from the 0.05% and 0.1% solutions show agreement with the theory of Jeon and Mullin; however, no deviation of D1 from that expected in an unpolarized mixture was observed because the3He is not in the degenerate regime for these very dilute systems for the temperatures we could achieve. The analysis of our measurements in terms of the Leggett-Rice equations also yields values for the spin rotation parameter μM0. Using our results along with previous measurements at various3He concentrations, we deduce a value for the s-wave quasiparticle scattering length of a=-0.88 ± 0.05 Å.

  20. Neutron Diffuse Reflectometry of Magnetic Thin Films with a 3He Analyzer

    NASA Astrophysics Data System (ADS)

    Chen, Wangchun; O'Donovan, Kevin; Borchers, Julie

    2005-03-01

    Polarized neutron reflectometry (PNR) is a powerful probe that characterizes the magnetization depth profile and magnetic domains in magnetic thin films. Although the conventionally used supermirrors are well-matched for specular PNR, they have limited angular acceptance and hence are impractical for complete characterization of the magnetic off-specular scattering where polarization analysis for diffusely reflected neutrons is required. Polarized ^3He gas, produced by optical pumping, can be used to polarize or analyze neutron beams because of the strong spin dependence of the neutron absorption cross section for ^3He. Here we report efficient polarization analysis of diffusely reflected neutrons in a reflectometry geometry using a polarized ^3He analyzer in conjunction with a position-sensitive detector (PSD). We obtained spin-resolved two-dimensional Qx-Qz reciprocal space maps for a patterned array of Co antidots in both the saturated and the demagnetized states. The preliminary results for a patterned amorphous bilayer, Gd40Fe60/ Tb55Fe45, measured with a ^3He analyzer and a PSD will also be discussed. Using the spin exchange optical pumping method we have achieved record high ^3He polarizations of 76% on the neutron beam line where we measured an initial analyzing efficiency of 0.97 and a neutron transmission for the desired spin state of 0.45.

  1. p +d →3He+γ reaction with pionless effective field theory

    NASA Astrophysics Data System (ADS)

    Nematollahi, H.; Bayegan, S.; Mahboubi, N.; Arani, M. Moeini

    2016-11-01

    We study the proton radiative capture by a deuteron with the pionless effective field theory [EFT(π / )] formalism. The calculation of the p d →3Heγ amplitude is considered for the incoming doublet and quartet channels leading to the formation of a 3He. The strong and Coulomb scattering amplitudes for the proton-deuteron (p d ) scattering are included in this study. In this calculation, the properly normalized 3He wave function has been used at each order. We evaluate both M 1 and E 1 transitions in the p d →3Heγ process up to NLO. We calculate the total cross section for the p d →3Heγ process based on the cluster-configuration space and compare it with the experimental data. The cross section results are presented for the incoming proton with the energy 0.5 ≤E ≤3 MeV where the lower and upper limits are chosen for the treatment of Coulomb effects perturbatively and the EFT(π / ) breakdown scale, respectively. No three-body force is needed to renormalize observables up to NLO other than those we have introduced in the p d scattering amplitudes.

  2. Association of 3He-Rich Solar Energetic Particles with Large-scale Coronal Waves

    NASA Astrophysics Data System (ADS)

    Bučík, Radoslav; Innes, Davina E.; Mason, Glenn M.; Wiedenbeck, Mark E.

    2016-12-01

    Small, 3He-rich solar energetic particle (SEP) events have been commonly associated with extreme-ultraviolet (EUV) jets and narrow coronal mass ejections (CMEs) that are believed to be the signatures of magnetic reconnection, involving field lines open to interplanetary space. The elemental and isotopic fractionation in these events are thought to be caused by processes confined to the flare sites. In this study, we identify 32 3He-rich SEP events observed by the Advanced Composition Explorer, near the Earth, during the solar minimum period 2007-2010, and we examine their solar sources with the high resolution Solar Terrestrial Relations Observatory (STEREO) EUV images. Leading the Earth, STEREO-A has provided, for the first time, a direct view on 3He-rich flares, which are generally located on the Sun’s western hemisphere. Surprisingly, we find that about half of the 3He-rich SEP events in this survey are associated with large-scale EUV coronal waves. An examination of the wave front propagation, the source-flare distribution, and the coronal magnetic field connections suggests that the EUV waves may affect the injection of 3He-rich SEPs into interplanetary space.

  3. OBSERVATIONS OF EUV WAVES IN {sup 3}He-RICH SOLAR ENERGETIC PARTICLE EVENTS

    SciTech Connect

    Bucík, R.; Innes, D. E.; Guo, L.; Mason, G. M.; Wiedenbeck, M. E.

    2015-10-10

    Small {sup 3}He-rich solar energetic particle (SEP) events with their anomalous abundances, markedly different from the solar system, provide evidence for a unique acceleration mechanism that operates routinely near solar active regions. Although the events are sometimes accompanied by coronal mass ejections (CMEs), it is believed that mass and isotopic fractionation is produced directly in the flare sites on the Sun. We report on a large-scale extreme-ultraviolet (EUV) coronal wave observed in association with {sup 3}He-rich SEP events. In the two examples discussed, the observed waves were triggered by minor flares and appeared concurrently with EUV jets and type III radio bursts, but without CMEs. The energy spectra from one event are consistent with so-called class-1 (characterized by power laws) {sup 3}He-rich SEP events, while the other with class-2 (characterized by rounded {sup 3}He and Fe spectra), suggesting different acceleration mechanisms in the two. The observation of EUV waves suggests that large-scale disturbances, in addition to more commonly associated jets, may be responsible for the production of {sup 3}He-rich SEP events.

  4. Results on Double-polarization Asymmetries in Quasielastic Scattering from Polarized 3He

    SciTech Connect

    Sulkosky, Vincent A.

    2016-03-01

    The 3He nucleus has become extremely important in the investigation of the neutron’s spin structure. When polarized, 3He acts as an effective polarized neutron target and hence facilitates our understanding of the neutron’s internal structure. However, to be used in this manner, our understanding of the internal structure of 3He is of extreme importance. As the precision of experiments has improved, the extraction of polarized neutron information from 3He leads to an ever larger share of the systematic uncertainty for these experiments. In these proceedings, I present a precise measurement of beam-target asymmetries in the and reactions. The former process is a uniquely sensitive probe of hadron dynamics in 3He and the structure of the underlying electromagnetic currents. The measurements have been performed around the quasi-elastic peak at Q2 = 0.25 (GeV/c)2 and 0.35 (GeV/c)2 for recoil momenta up to 270 MeV/c. The experimental apparatus, analysis and results were presented together with a comparison to state-of-the art Faddeev calculations.

  5. MiRNA molecular profiles in human medical conditions: connecting lung cancer and lung development phenomena.

    PubMed

    Aghanoori, Mohamad-Reza; Mirzaei, Behnaz; Tavallaei, Mahmood

    2014-01-01

    MiRNAs are endogenous, single stranded ~22-nucleotide non-coding RNAs (ncRNAs) which are transcribed by RNA polymerase II and mediate negative post-transcriptional gene regulation through binding to 3'untranslated regions (UTR), possibly open reading frames (ORFs) or 5'UTRs of target mRNAs. MiRNAs are involved in the normal physiology of eukaryotic cells, so dysregulation may be associated with diseases like cancer, and neurodegenerative, heart and other disorders. Among all cancers, lung cancer, with high incidence and mortality worldwide, is classified into two main groups: non-small cell lung cancer and small cell lung cancer. Recent promising studies suggest that gene expression profiles and miRNA signatures could be a useful step in a noninvasive, low-cost and repeatable screening process of lung cancer. Similarly, every stage of lung development during fetal life is associated with specific miRNAs. Since lung development and lung cancer phenomena share the same physiological, biological and molecular processes like cell proliferation, development and shared mRNA or expression regulation pathways, and according to data adopted from various studies, they may have partially shared miRNA signature. Thus, focusing on lung cancer in relation to lung development in miRNA studies might provide clues for lung cancer diagnosis and prognosis.

  6. The Effect Of Neutron Attenuation On Power Deposition In Nuclear Pumped 3He-Lasers

    SciTech Connect

    Cetin, Fuesun

    2007-04-23

    Nuclear-pumped lasers (NPLs) are driven by the products of nuclear reactions and directly convert the nuclear energy to directed optical energy. Pumping gas lasers by nuclear reaction products has the advantage of depositing large energies per reaction. The need for high laser power output implies high operating pressure. In the case of volumetric excitation by 3He(n, p)3H reactions, however, operation at high pressure (more than a few atm) causes excessive neutron attenuation in the 3He gas. This fact adversely effects on energy deposition and, hence, laser output power and beam quality. Here, spatial and temporal variations of neutron flux inside a closed 3He -filled cylindrical laser tube have been numerically calculated for various tube radii and operating pressures by using a previously reported dynamic model for energy deposition. Calculations are made by using ITU TRIGA Mark II Reactor as the neutron source. The effects of neutron attenuation on power deposition are examined.

  7. The Feasibility Of Fusion Reactors Fueled With D-{sup 3}He And D-D

    SciTech Connect

    Stott, Peter

    2009-10-08

    In this paper we discuss the feasibility of fusion reactors based on D-{sup 3}He and D-D fuel mixtures. The low reactivity of the D-{sup 3}He and D-D fusion reactions and the large energy losses due to bremsstrahlung and synchrotron radiation at high plasma temperatures severely restricts the choice of fuel mixtures that can be brought to ignition. These fuel mixtures are extremely sensitive to impurities and to helium ash retention and they would require reactor conditions (plasma density, temperature, beta and energy confinement time) that are much more demanding than the requirements for D-T. A reactor burning D-{sup 3}He or D-D would be far beyond the most optimistic extrapolations of known magnetic confinement schemes, it would have problems with sustainable fuel supplies and it would produce substantial numbers of neutrons. Our conclusion is that these fuels cannot be considered as realistic alternatives to D-T.

  8. A novel method to measure low flux ambient thermal neutrons with 3He proportional counters

    NASA Astrophysics Data System (ADS)

    Zeng, Z. M.; Gong, H.; Yue, Q.; Li, J. M.

    2017-09-01

    A pulse shape discrimination method to discriminate neutron events from backgrounds based on the double-pulse effect of 3He proportional counters is proposed and detailed in this paper. We made an ambient thermal neutron measurement system composed of a commercial 3He proportional counter tube and the corresponding readout electronics. The background of the system has been measured and the minimum detectable amount of the 3He proportional counter tube will be reduced by an order of magnitude with this method. The system was applied to measure the ambient thermal neutron flux inside a large neutron shielding structure at a deep underground laboratory and the pulse shape discrimination method proves to be effective.

  9. Comparative study of nuclear effects in polarized electron scattering from 3 He

    DOE PAGES

    Ethier, J. J.; Melnitchouk, W.

    2013-11-01

    We present a detailed analysis of nuclear effects in inclusive electron scattering from polarized 3He nuclei for polarization asymmetries, structure functions and their moments, both in the nucleon resonance and deep-inelastic regions. We compare the results of calculations within the weak binding approximation at finite Q2 with the effective polarization ansatz often used in experimental data analyses, and explore the impact of Δ components in the nuclear wave function and nucleon off-shell corrections on extractions of the free neutron structure. Using the same framework we also make predictions for the Q2 dependence of quasielastic scattering from polarized 3He, data onmore » which can be used to constrain the spin-dependent nuclear smearing functions in 3He.« less

  10. The Neutron and 3He Spin Structure Functions at Low Q^2

    SciTech Connect

    Vincent Sulkosky

    2009-08-01

    Experiment E97-110 was performed at the Thomas Jefferson National Accelerator Facility in Hall A to provide a precise measurement of the moments of the neutron and $^{3}$He spin structure functions. A longitudinally-polarized electron beam was scattered from a longitudinally or transversely polarized $^{3}$He target. The extended Gerasimov-Drell-Hearn integral and other moments of the neutron and $^{3}$He spin structure functions were extracted at very low momentum transfers (0.02 $< Q^{2} <$ 0.3 [GeV$/c$]$^{2}$). These data allow us to make a benchmark check of Chiral Perturbation Theory calculations in a region where they are expected to be valid. In these proceedings, the experimental details are discussed and preliminary results on the moments of the spin structure functions are presented.

  11. Observation of a new superfluid phase for 3He embedded in nematically ordered aerogel

    NASA Astrophysics Data System (ADS)

    Zhelev, N.; Reichl, M.; Abhilash, T. S.; Smith, E. N.; Nguyen, K. X.; Mueller, E. J.; Parpia, J. M.

    2016-09-01

    In bulk superfluid 3He at zero magnetic field, two phases emerge with the B-phase stable everywhere except at high pressures and temperatures, where the A-phase is favoured. Aerogels with nanostructure smaller than the superfluid coherence length are the only means to introduce disorder into the superfluid. Here we use a torsion pendulum to study 3He confined in an extremely anisotropic, nematically ordered aerogel consisting of ~10 nm-thick alumina strands, spaced by ~100 nm, and aligned parallel to the pendulum axis. Kinks in the development of the superfluid fraction (at various pressures) as the temperature is varied correspond to phase transitions. Two such transitions are seen in the superfluid state, and we identify the superfluid phase closest to Tc at low pressure as the polar state, a phase that is not seen in bulk 3He.

  12. Magnetic field dependent transverse spin diffusion constant in 3He- 4He solutions

    NASA Astrophysics Data System (ADS)

    Owers-Bradley, J. R.; Child, A.; Bowley, R. M.

    1994-02-01

    The transverse spin diffusion constant of 3He- 4He solutions has been measured by pulsed nmr in magnetic fields of 2.18T and 8.8T for 3He concentrations of 0.5%, 1.0% and 3.8%. For the higher concentrations the diffusion constant at 8.8T is smaller than at 2.18T for the lowest temperatures used. The effect is largest for the 3.8% solution (a reduction by 1.7 at 15mK), but is too small to be measurable for the 0.5% solution. These results are compared to measurements of Candela et al. for pure 3He, and to the theory of Jeon and Mullin.

  13. A Short History of the Theory and Experimental Discovery of Superfluidity in 3He

    NASA Astrophysics Data System (ADS)

    Brinkman, W. F.

    I discuss the development of the theory and experiments on superfluid 3He. After the discovery of superfluidity in 3He by Osheroff, Richardson and Lee, Phil Anderson quickly recruited Doug Osheroff to come to Bell Labs and set up a dilution fridge to continue his experiments. One of the mysteries at that time was how the high-temperature A-phase, which has a gapless excitation spectrum, could be stabilized relative to the fully gapped, lower temperature B-phase. I explain how Phil Anderson and I developed the spin fluctuation theory of the A-phase of superfluid 3He which accounted for its stability, leading to the Anderson-Brinkman-Morel (ABM) theory of the superfluid A-phase...

  14. Developing a long duration 3He fridge for the LSPE-SWIPE instrument

    NASA Astrophysics Data System (ADS)

    Coppi, Gabriele; de Bernardis, Paolo; May, Andrew J.; Masi, Silvia; McCulloch, Mark; Melhuish, Simon J.; Piccirillo, Lucio

    2016-07-01

    A 3He sorption cooler design for the Short-Wavelength Instrument for the Polarization Explorer (SWIPE) of the Large-Scale Polarization Explorer (LSPE) balloon-borne experiment is described. The aim of this experiment is the detection of the primordial B-mode polarisation component of the Cosmic Microwave Background. The SWIPE instrument will use Transition-Edge Sensors that are designed to work at temperature of almost 300 mK. Therefore, a 3He sorption cooler has been specifically designed that can reach this temperature with a heat load of up to 25 μW. The fridge is compact in order to be housed inside the SWIPE cryostat and operate vertically. It has been designed to have a cycle duration of at least 7 days. In order to meet these specifications, the fridge will be charged with 0.75 moles of 3He.

  15. Rotational spectrum of the NH3-He van der Waals complex

    NASA Astrophysics Data System (ADS)

    Surin, L.; Schnell, M.

    2016-12-01

    The interaction between ammonia and helium has attracted considerable interest over many years, partly because of the observation of interstellar ammonia. The rate coefficients of NH3-He scattering are an important ingredient for numerical modeling of astrochemical environments. Another, though quite different application in which the NH3-He interaction can play an important role is the doping of helium clusters with NH3 molecules to perform high-resolution spectroscopy. Such experiments are directed on the detection of non-classical response of molecular rotation in helium clusters addressing fundamental questions related to the microscopic nature of superfluidity. High-resolution spectroscopy on the NH3-He complex is an important tool for increasing our understanding of intermolecular forces between NH3 and He.

  16. Heavy-baryon chiral perturbation theory approach to thermal neutron capture on {sup 3}He

    SciTech Connect

    Lazauskas, Rimantas; Park, Tae-Sun

    2011-03-15

    The cross section for radiative thermal neutron capture on {sup 3}He ({sup 3}He+n{yields}{sup 4}He+{gamma}; known as the hen reaction) is calculated based on heavy-baryon chiral perturbation theory. The relevant M1 operators are derived up to next-to-next-to-next-to-leading order (N{sup 3}LO). The initial and final nuclear wave functions are obtained from the rigorous Faddeev-Yakubovski equations for five sets of realistic nuclear interactions. Up to N{sup 3}LO, the M1 operators contain two low-energy constants, which appear as the coefficients of nonderivative two-nucleon contact terms. After determining these two constants using the experimental values of the magnetic moments of the triton and {sup 3}He, we carry out a parameter-free calculation of the hen cross section. The results are in good agreement with the data.

  17. Observation of a new superfluid phase for 3He embedded in nematically ordered aerogel

    PubMed Central

    Zhelev, N.; Reichl, M.; Abhilash, T. S.; Smith, E. N.; Nguyen, K. X.; Mueller, E. J.; Parpia, J. M.

    2016-01-01

    In bulk superfluid 3He at zero magnetic field, two phases emerge with the B-phase stable everywhere except at high pressures and temperatures, where the A-phase is favoured. Aerogels with nanostructure smaller than the superfluid coherence length are the only means to introduce disorder into the superfluid. Here we use a torsion pendulum to study 3He confined in an extremely anisotropic, nematically ordered aerogel consisting of ∼10 nm-thick alumina strands, spaced by ∼100 nm, and aligned parallel to the pendulum axis. Kinks in the development of the superfluid fraction (at various pressures) as the temperature is varied correspond to phase transitions. Two such transitions are seen in the superfluid state, and we identify the superfluid phase closest to Tc at low pressure as the polar state, a phase that is not seen in bulk 3He. PMID:27669660

  18. New apparatus for the precise temperature control in 3He refrigerator

    NASA Astrophysics Data System (ADS)

    Kato, Kiyonori; Yoshida, Hisashi; Suzui, Mitsukazu

    1998-01-01

    A new, simple, and low cost apparatus was developed to control the temperature in the very low-temperature region (0.3-3.3 K) using a 3He refrigerator. A low temperature was obtained by controlling the 3He vapor pressure. The apparatus consists of a set of valves, an automated microvalve, a manually operated auxiliary valve, a controller including a one-board microcomputer, and a set of pressure transducers. The temperature was easily controlled by this apparatus. The temperature stability was 0.95% at 1.04 K by controlling the 3He vapor pressure. The temperature drift was typically 0.02 K at 0.85 K.

  19. Implications of new High 3He/4He Values from the Samoan Hotspot

    NASA Astrophysics Data System (ADS)

    Jackson, M. G.; Kurz, M. D.; Hart, S. R.; Workman, R.

    2005-12-01

    We report new olivine phenocryst helium measurements from Ofu Island, American Samoa; the 3He/4He ratios range from 19.5 to 33.7 times atmospheric (R/Ra), significantly expanding the observed range for Samoa. The highest 3He/4He ratio of 33.7 Ra was measured in olivines from an ankaramite dike. Relatively high helium concentrations (4.4*108 cc/g) in this sample, crushing and fusion measurements, coupled with sampling from a roadcut exposure, all ensure that the isotope ratio is not affected by in situ cosmogenic 3He. A second basaltic dike yielded a 3He/4He ratio of 29.6 Ra, and measurements on 9 other basalt samples from Ofu range from 19.5 to 26.4 Ra. Previous high 3He/4He measurements (~25 Ra) from the Samoan hotspot were also obtained from basaltic dikes, but were from Tutuila Island (Farley et al., 1992). The new high 3He/4He ratios from Samoa are similar in magnitude to the high ratios found at Iceland (~37 Ra) and Hawaii (~35 Ra). However, the Ofu basalts have 87Sr/86Sr > 0.7044, which is significantly more radiogenic than Iceland or Hawaii. The combined Sr-He isotopic data are broadly consistent with mixing between an enriched mantle source (EM2) and the putative common high 3He/4He component (FOZO, as best represented by Baffin Island Picrites, Stuart et al., 2003). Assuming that the overall isotopic variations are produced by mixing processes, we attempt to place constraints on the relative helium concentrations in the FOZO, EM2 and Depleted MORB mantle (DMM) endmembers. In addition to using the shape of the plausible mixing lines, we employ new estimates for the trace element concentrations in the DMM and Samoan EM2 sources (Workman et al., 2004; Workman and Hart, 2005) to get at relative helium concentrations in these reservoirs. We assume that high 3He/4He basalts from the mid-Atlantic ridge North of Iceland (Schilling et al., 1999, and others) are a mixture between DMM and FOZO. We further assume that the N. Iceland ridge-FOZO and Ofu-FOZO mixing

  20. Longitudinal assessment of treatment effects on pulmonary ventilation using 1H/3He MRI multivariate templates

    NASA Astrophysics Data System (ADS)

    Tustison, Nicholas J.; Contrella, Benjamin; Altes, Talissa A.; Avants, Brian B.; de Lange, Eduard E.; Mugler, John P.

    2013-03-01

    The utitlity of pulmonary functional imaging techniques, such as hyperpolarized 3He MRI, has encouraged their inclusion in research studies for longitudinal assessment of disease progression and the study of treatment effects. We present methodology for performing voxelwise statistical analysis of ventilation maps derived from hyper­ polarized 3He MRI which incorporates multivariate template construction using simultaneous acquisition of IH and 3He images. Additional processing steps include intensity normalization, bias correction, 4-D longitudinal segmentation, and generation of expected ventilation maps prior to voxelwise regression analysis. Analysis is demonstrated on a cohort of eight individuals with diagnosed cystic fibrosis (CF) undergoing treatment imaged five times every two weeks with a prescribed treatment schedule.

  1. The Feasibility Of Fusion Reactors Fueled With D-3He And D-D

    NASA Astrophysics Data System (ADS)

    Stott, Peter

    2009-10-01

    In this paper we discuss the feasibility of fusion reactors based on D-3He and D-D fuel mixtures. The low reactivity of the D-3He and D-D fusion reactions and the large energy losses due to bremsstrahlung and synchrotron radiation at high plasma temperatures severely restricts the choice of fuel mixtures that can be brought to ignition. These fuel mixtures are extremely sensitive to impurities and to helium ash retention and they would require reactor conditions (plasma density, temperature, beta and energy confinement time) that are much more demanding than the requirements for D-T. A reactor burning D-3He or D-D would be far beyond the most optimistic extrapolations of known magnetic confinement schemes, it would have problems with sustainable fuel supplies and it would produce substantial numbers of neutrons. Our conclusion is that these fuels cannot be considered as realistic alternatives to D-T.

  2. d-3 He reaction measurements during fast wave minority heating in the PLT tokamak experiment

    NASA Astrophysics Data System (ADS)

    Chrien, R. E.; Strachan, J. D.

    1983-07-01

    Time- and energy-resolved d-3He fusion reactions have been measured to infer the energy of the d+ or He++ minority ions heated near their cyclotron frequency by the magnetosonic fast wave. The average energy of the reacting 3He ions during 3He minority heating is in the range of 100-400 keV, as deduced from the magnitude of the reaction rate, its decay time, and the energy spread of the proton reaction products. The observed reaction rate and its scaling with wave power and electron density and temperature are in qualitative agreement with a radial reaction rate model using the minority distribution predicted from quasilinear velocity space diffusion. Oscillations in the reaction rate are observed concurrent with sawtooth and m=2 magnetohydrodynamic activity in the plasma.

  3. Phase separation in dilute solutions of 3He in solid 4He

    NASA Astrophysics Data System (ADS)

    Huan, C.; Yin, L.; Xia, J. S.; Candela, D.; Cowan, B. P.; Sullivan, N. S.

    2017-03-01

    We report the results of studies of the phase separation of solid solutions of dilute concentrations of 3He in 4He. The temperatures and the kinetics of the phase separation were determined from NMR experiments for 3He concentrations 1.6 ×10-53He droplets shows a t1 /3 time dependence at long times consistent with Ostwald ripening.

  4. A 3He counter version of the Thermo Fisher Scientific NRD neutron rem meter.

    PubMed

    Olsher, Richard H; Seagraves, David T

    2008-01-01

    Thermo Fisher Scientific's NRD rem meter has been in production for almost 40 y and is the primary rem meter in use at many U.S. Department of Energy facilities. An upgrade project was initiated at the Los Alamos National Laboratory with the primary goal of increasing the NRD's neutron sensitivity through the substitution of pressurized 3He gas (4 atmospheres) for the stock counter tube's BF3 fill gas. Historically, BF3 counters were far less expensive relative to 3He and were usually chosen on the basis of cost. That is no longer the case, with pricing for both types of counters being similar. Test results have shown that the 3He counter version of the NRD exhibits stable operation at a reasonable bias voltage and good gamma rejection. Sensitivity has been increased by about a factor of four with no penalty in cost.

  5. Chiral effective field theory predictions for muon capture on deuteron and {3}He.

    PubMed

    Marcucci, L E; Kievsky, A; Rosati, S; Schiavilla, R; Viviani, M

    2012-02-03

    The muon-capture reactions {2}H(μ{-},ν{μ})nn and {3}He(μ{-},ν{μ}){3}H are studied with nuclear potentials and charge-changing weak currents, derived in chiral effective field theory. The low-energy constants (LECs) c{D} and c{E}, present in the three-nucleon potential and (c{D}) axial-vector current, are constrained to reproduce the A=3 binding energies and the triton Gamow-Teller matrix element. The muon-capture rates on deuteron and {3}He are predicted to be 399±3  sec{-1} and 1494±21  sec{-1}, respectively. The spread accounts for the cutoff sensitivity, as well as uncertainties in the LECs and electroweak radiative corrections. By comparing the calculated and precisely measured rates on {3}He, a value for the induced pseudoscalar form factor is obtained in good agreement with the chiral perturbation theory prediction.

  6. Use of .sup.3 He.sup.30 + ICRF minority heating to simulate alpha particle heating

    DOEpatents

    Post, Jr., Douglass E.; Hwang, David Q.; Hovey, Jane

    1986-04-22

    Neutron activation due to high levels of neutron production in a first heated deuterium-tritium plasma is substantially reduced by using Ion Cyclotron Resonance Frequency (ICRF) heating of energetic .sup.3 He.sup.++ ions in a second deuterium-.sup.3 He.sup.++ plasma which exhibit an energy distribution and density similar to that of alpha particles in fusion reactor experiments to simulate fusion alpha particle heating in the first plasma. The majority of the fast .sup.3 He.sup.++ ions and their slowing down spectrum can be studied using either a modulated hydrogen beam source for producing excited states of He.sup.+ in combination with spectrometers or double charge exchange with a high energy neutral lithium beam and charged particle detectors at the plasma edge. The maintenance problems thus associated with neutron activation are substantially reduced permitting energetic alpha particle behavior to be studied in near term large fusion experiments.

  7. Intercalibration of 3He and Other Cosmogenic Nuclide Production Rates In Multiple Mineral Phases

    NASA Astrophysics Data System (ADS)

    Farley, K. A.; Amidon, W. H.; Renne, P. R.; Simon, J. I.; Burbank, D. W.

    2007-12-01

    To extend the applicability of cosmogenic 3He dating beyond minerals restricted to mafic rocks (i.e., olivine and pyroxene), we have been assessing the suitability of 3He dating of additional minerals, e.g., zircon. A key aspect of this undertaking is the calibration of spallation production rates. Because the production rate of 3He varies from element to element in a fashion that is not yet well known, an empirical approach is necessary. Our recent work has focused on the Devil's Kitchen rhyolite, Coso Volcanic Field, SE California. This rhyolite has a 587 ka K/Ar age, which will be verified with a new 40Ar/39Ar age on sanidine. The attraction of this rhyolite is its extraordinary mineral assemblage arising from both the rhyolite itself and abundant mafic inclusions. From two individual rocks we obtained separates and measured 3He in the following minerals: olivine, clinopyroxene, orthopyroxene, garnet, zircon, apatite, hornblende, and ilmenite. In addition we intend to measure 21Ne in quartz and sanidine, 38Ar in sanidine, and 10Be and 26Al in quartz. We sampled two surfaces: one with tension gashes documenting an original flow surface, and a protruding rock fin. The sample with primary surface morphology is apparently uneroded, but preliminary 3He analyses of olivine suggest the surface has been buried for a substantial part of its history. Olivine 3He analyses from the fin indicate exposure ages consistent with the eruption age, so this sample may allow absolute calibration of production rates. In either case we can use these samples to inter-calibrate production rates in a large number of minerals using multiple cosmogenic isotopes.

  8. Characterizing a sewage plume using the 3H-3He dating technique

    USGS Publications Warehouse

    Shapiro, Stephanie Dunkle; LeBlanc, Denis; Schlosser, Peter; Ludin, Andrea

    1999-01-01

    An extensive 3H-3He study was performed to determine detailed characteristics of a regional flow system and a sewage plume over a distance of 4 km in a sand and gravel aquifer at Otis Air Base in Falmouth, Massachusetts. 3H-3He ages increase with depth in individual piezometer clusters and with distance along flowpaths. However, the age gradient with depth (Δt/Δz) is smaller in the plume than that in the regional waters, due to the intense recharge in the infiltration beds. The 1960s bomb peak of tritium in precipitation is archived longitudinally along a flowline through the main axis of the plume and vertically in individual piezometer clusters. On the eastern side of the sampling area, where water from Ashumet Pond forces plume water deeper into the flow system, 3H-3He ages are young at depth because the 3H-3He "clock" is reset due to outgassing of helium in the pond. A reconstruction of the tritium input functions for the regional and plume samples shows that there is no offset in the peak [3H]+[3Hetrit] concentrations for the plume and regional water, indicating that the water from supply wells for use on the base is young. The 3H-3He ages and detergent concentrations in individual wells are consistent with the beginning of use of detergents and the time period when their concentrations in sewage would have been greatest. Ages and hydraulic properties calculated using the 3H-3He data compare well with those from previous investigations and from particle-tracking simulations.

  9. Boron-coated straws as a replacement for 3He-based neutron detectors

    NASA Astrophysics Data System (ADS)

    Lacy, Jeffrey L.; Athanasiades, Athanasios; Sun, Liang; Martin, Christopher S.; Lyons, Tom D.; Foss, Michael A.; Haygood, Hal B.

    2011-10-01

    US and international government efforts to equip major seaports with large area neutron detectors, aimed to intercept the smuggling of nuclear materials, have precipitated a critical shortage of 3He gas. It is estimated that the annual demand of 3He for US security applications alone is more than the worldwide supply. This is strongly limiting the prospects of neutron science, safeguards, and other applications that rely heavily on 3He-based detectors. Clearly, alternate neutron detection technologies that can support large sensitive areas, and have low gamma sensitivity and low cost must be developed. We propose a low-cost technology based on long copper tubes (straws), coated on the inside with a thin layer of 10B-enriched boron carbide ( 10B 4C). In addition to the high abundance of boron on Earth and low cost of 10B enrichment, the boron-coated straw (BCS) detector offers distinct advantages over conventional 3He-based detectors, and alternate technologies such as 10BF 3 tubes and 10B-coated rigid tubes. These include better distribution inside moderator assemblies, many-times faster electronic signals, no pressurization, improved gamma-ray rejection, no toxic or flammable gases, and ease of serviceability. We present the performance of BCS detectors dispersed in a solid plastic moderator to address the need for portal monitoring. The design adopts the outer dimensions of currently deployed 3He-based monitors, but takes advantage of the small BCS diameter to achieve a more uniform distribution of neutron converter throughout the moderating material. We show that approximately 63 BCS detectors, each 205 cm long, distributed inside the moderator, can match or exceed the detection efficiency of typical monitors fitted with a 5 cm diameter 3He tube, 187 cm long, pressurized to 3 atm.

  10. JET (3He)-D scenarios relying on RF heating: survey of selected recent experiments

    SciTech Connect

    Van Eester, D.; Casati, A.; Crombe, K.; de la Luna, E.; Ericsson, G.; Felton, R.; Giroud, C.; Hjalmarsson, A.; Joffrin, E.; Kallne, J.; Kiptily, V.; Marinoni, A.; Santala, M.; Valisa, M.

    2009-03-01

    Recent JET experiments have been devoted to the study of (3He) D plasmas involving radio frequency (RF) heating. This paper starts by discussing the RF heating efficiency theoretically expected in such plasmas, covering both relevant aspects of wave and of particle dynamics. Then it gives a concise summary of the main conclusions drawn from recent experiments that were either focusing on studying RF heating physics aspects or that were adopting RF heating as a tool to study plasma behavior. Depending on the minority concentration chosen, different physical phenomena are observed. At very low concentration (X[3He] < 1%), energetic tails are formed which trigger MHD activity and result in loss of fast particles. Alfv n cascades were observed and gamma ray tomography indirectly shows the impact of sawtooth crashes on the fast particle orbits. Low concentration (X[3He] < 10%) favors minority heating while for X[3He] 10% electron mode conversion damping becomes dominant. Evidence for the Fuchs et al standing wave effect (Fuchs et al 1995 Phys. Plasmas 2 1637 47) on the absorption is presented. RF induced deuterium tails were observed in mode conversion experiments with large X[3He] (18%). As tentative modeling shows, the formation of these tails can be explained as a consequence of wave power absorption by neutral beam particles that efficiently interact with the waves well away from the cold D cyclotron resonance position as a result of their substantial Doppler shift. As both ion and electron RF power deposition profiles in (3He) D plasmas are fairly narrow giving rise to localized heat sources the RF heating method is an ideal tool for performing transport studies. Various of the experiments discussed here were done in plasmas with internal transport barriers (ITBs). ITBs are identified as regions with locally reduced diffusivity, where poloidal spinning up of the plasma is observed. The present know-how on the role of RF heating for impurity transport is also

  11. MeV ion loss during sup 3 He minority heating in TFTR

    SciTech Connect

    Zweben, S.J.; Hammett, G.; Boivin, R.; Phillips, C.; Wilson, R.

    1992-01-01

    The loss of MeV ions during {sup 3}He ICRH minority heating experiments has been measured using scintillator detectors near the wall of TFTR. The observed MeV ion losses to the bottom (90{degrees} poloidal) detector are generally consistent with the expected first-orbit loss of D-{sup 3}He alpha particle fusion products, with an inferred global reaction rate up to {approx}10{sup 16} reactions/sec. A qualitatively similar but unexpectedly large loss occurs 45{degrees} poloidally below the outer midplane. This additional loss might be due to ICRH tail ions or to ICRH wave-induced loss of previously confined fusion products.

  12. MeV ion loss during {sup 3}He minority heating in TFTR

    SciTech Connect

    Zweben, S.J.; Hammett, G.; Boivin, R.; Phillips, C.; Wilson, R.

    1992-01-01

    The loss of MeV ions during {sup 3}He ICRH minority heating experiments has been measured using scintillator detectors near the wall of TFTR. The observed MeV ion losses to the bottom (90{degrees} poloidal) detector are generally consistent with the expected first-orbit loss of D-{sup 3}He alpha particle fusion products, with an inferred global reaction rate up to {approx}10{sup 16} reactions/sec. A qualitatively similar but unexpectedly large loss occurs 45{degrees} poloidally below the outer midplane. This additional loss might be due to ICRH tail ions or to ICRH wave-induced loss of previously confined fusion products.

  13. Observation of Half-Quantum Vortices in Topological Superfluid ^{3}He.

    PubMed

    Autti, S; Dmitriev, V V; Mäkinen, J T; Soldatov, A A; Volovik, G E; Yudin, A N; Zavjalov, V V; Eltsov, V B

    2016-12-16

    One of the most sought-after objects in topological quantum-matter systems is a vortex carrying half a quantum of circulation. They were originally predicted to exist in superfluid ^{3}He-A but have never been resolved there. Here we report an observation of half-quantum vortices (HQVs) in the polar phase of superfluid ^{3}He. The vortices are created with rotation or by the Kibble-Zurek mechanism and identified based on their nuclear magnetic resonance signature. This discovery provides a pathway for studies of unpaired Majorana modes bound to the HQV cores in the polar-distorted A phase.

  14. Pion single charge exchange scattering from 3He at 285, 428, and 525 MeV

    NASA Astrophysics Data System (ADS)

    Källne, J.; Altemus, R.; Gugelot, P. C.; McCarthy, J. S.; Minehart, R. C.; Orphanos, L.; Gram, P. A. M.; Höistad, B.; Morris, C. L.; Wadlinger, E. A.; Perdrisat, C. F.

    1982-02-01

    We have measured the cross section of 3He(π-,π0)3H at T=285, 428, and 525 MeV for angles in the range of 60°<~θ<~135° covering the momentum transfer range 0.5<~q<~1.0 GeV/c. Comparison is made with Glauber model calculations to discuss the sensitivity to nuclear structure and pion-nucleus interaction effects. NUCLEAR REACTIONS π-+3He-->3H+π0, T=285, 428, and 525 MeV, θπ0~70-140° measured σ(θt,Tπ). Analysis based on optical and Glauber model predictions.

  15. Tricritical Casimir forces and order parameter profiles in wetting films of ^{3}He-^{4}He mixtures.

    PubMed

    Farahmand Bafi, N; Maciołek, A; Dietrich, S

    2017-03-01

    Tricritical Casimir forces in ^{3}He-^{4}He wetting films are studied, within mean field theory, in terms of a suitable lattice gas model for binary liquid mixtures with short-ranged surface fields. The proposed model takes into account the continuous rotational symmetry O(2) of the superfluid degrees of freedom associated with ^{4}He and it allows, inter alia, for the occurrence of a vapor phase. As a result, the model facilitates the formation of wetting films, which provides a strengthened theoretical framework to describe available experimental data for tricritical Casimir forces acting in ^{3}He-^{4}He wetting films.

  16. Resonance transition 795-nm Rubidium laser using 3He buffer gas

    SciTech Connect

    Wu, S S; Soules, T F; Page, R H; Mitchell, S C; Kanz, V K; Beach, R J

    2007-08-02

    We report the first demonstration of a 795-nm Rubidium resonance transition laser using a buffer gas consisting of pure {sup 3}He. This follows our recent demonstration of a hydrocarbon-free 795-nm Rubidium resonance laser which used naturally-occurring He as the buffer gas. Using He gas that is isotopically enriched with {sup 3}He yields enhanced mixing of the Rb fine-structure levels. This enables efficient lasing at reduced He buffer gas pressure, improving thermal management in high average power Rb lasers and enhancing the power scaling potential of such systems.

  17. Measurement of H(dvec, 3He)γ reaction using a large acceptance spectrograph

    NASA Astrophysics Data System (ADS)

    Yagita, T.; Sagara, K.; Kondo, M.; Minami, S.; Ishida, T.; Hatanaka, K.; Wakasa, T.; Kamiya, J.; Hirooka, D.; Noro, T.; Yoshida, H. P.; Obayashi, E.; Takahisa, K.; Yoshimura, M.; Akiyoshi, H.

    2001-06-01

    A measurement of cross section and analyzing powers Ay, Ayy and Axx of H(dvec, 3He) reaction at Ed=200 MeV is in progress at RCNP. The target is liquid hydrogen of about 1.5 mm in thickness. Since 3He particles from the reaction are concentrated in very forward angles in the laboratory frame, we use a large acceptance spectrograph (LAS) to measure the reaction simultaneously from θcm=20° to 160° in the horizontal plane (Ay, Ayy and cross section) and in the vertical plane (Axx). The preliminary data are compared with 3N Faddeev calculations. .

  18. Two-body pion absorption on {sup 3}He at threshold

    SciTech Connect

    Lee, T.S.H.; Kiang, L.L.; Riska, D.O.

    1995-08-01

    We showed that a drastic reduction of the ratio of the rates of the reactions {sup 3}He({pi}{sup -},nn) and {sup 3}He({pi}{sup -},np) for stopped pions is obtained once the effect of the short-range two-nucleon components of the axial charge operator for nuclear systems is taken into account. In a calculation using realistic models of nucleon-nucleon interactions in the construction of these short-range components of the axial charge operator, the predicted ratios can be brought to within 10-20% of the empirical value. A paper describing our results was published.

  19. Two-photon exchange correction to 2 S -2 P splitting in muonic 3He ions

    NASA Astrophysics Data System (ADS)

    Carlson, Carl E.; Gorchtein, Mikhail; Vanderhaeghen, Marc

    2017-01-01

    We calculate the two-photon exchange correction to the Lamb shift in muonic 3He ions within the dispersion relations framework. Part of the effort entailed making analytic fits to the electron-3He quasielastic scattering data set, for purposes of doing the dispersion integrals. Our result is that the energy of the 2 S state is shifted downwards by two-photon exchange effects by 15.14(49) meV, in good accord with the result obtained from a potential model and effective field theory calculation.

  20. B phase with polar distortion in superfluid {sup 3}He in “ordered” aerogel

    SciTech Connect

    Dmitriev, V. V. Senin, A. A.; Soldatov, A. A.; Surovtsev, E. V.; Yudin, A. N.

    2014-12-15

    The properties of the low-temperature superfluid phase of {sup 3}He in “nematically ordered” aerogel in which strands are almost parallel to one another are investigated by nuclear magnetic resonance methods. Such a strong anisotropy of the aerogel affects the phase diagram of {sup 3}He and the structure of superfluid phases. A theoretical model of the B phase with polar distortion is developed. It is shown that this model successfully describes the observed properties of the low-temperature phase.

  1. Nuclear charge symmetry breaking and the 3H-3He binding energy difference

    NASA Astrophysics Data System (ADS)

    Brandenburg, R. A.; Chulick, G. S.; Kim, Y. E.; Klepacki, D. J.; Machleidt, R.; Picklesimer, A.; Thaler, R. M.

    1988-02-01

    We study the 3H- 3He binding energy difference, taking into account the Coulomb interaction and charge symmetry breaking of the nuclear force consistent with recent NN experimental data. Realistic interactions are generated which describe the charge symmetry violations reflected in the different nucleon-nucleon scattering lengths. The influence of nuclear charge symmetry breaking on the perturbative Coulomb contribution to the 3He binding energy is discussed. It is shown that the experimental mass difference can be explained by these and theoretical estimates of other known effects.

  2. SEOP polarized 3He Neutron Spin Filters for the JCNS user program

    NASA Astrophysics Data System (ADS)

    Babcock, Earl; Salhi, Zahir; Theisselmann, Tobias; Starostin, Denis; Schmeissner, Johann; Feoktystov, Artem; Mattauch, Stefan; Pistel, Patrick; Radulescu, Aurel; Ioffe, Alexander

    2016-04-01

    Over the past several years the JCNS has been developing in-house applications for neutron polarization analysis (PA). These methods include PA for separation of incoherent from coherent scattering in soft matter studies (SANS), and online polarization for analysis for neutron reflectometry, SANS, GISANS and eventually spectroscopy. This paper will present an overview of the user activities at the JCNS at the MLZ and gives an overview of the polarization 3He methods and devices used. Additionally we will summarise current projects which will further support the user activities using polarised 3He spin filters.

  3. Observation of Half-Quantum Vortices in Topological Superfluid 3He

    NASA Astrophysics Data System (ADS)

    Autti, S.; Dmitriev, V. V.; Mäkinen, J. T.; Soldatov, A. A.; Volovik, G. E.; Yudin, A. N.; Zavjalov, V. V.; Eltsov, V. B.

    2016-12-01

    One of the most sought-after objects in topological quantum-matter systems is a vortex carrying half a quantum of circulation. They were originally predicted to exist in superfluid 3He -A but have never been resolved there. Here we report an observation of half-quantum vortices (HQVs) in the polar phase of superfluid 3He. The vortices are created with rotation or by the Kibble-Zurek mechanism and identified based on their nuclear magnetic resonance signature. This discovery provides a pathway for studies of unpaired Majorana modes bound to the HQV cores in the polar-distorted A phase.

  4. Observation of d-3He Fusion Reactions in a Tokamak Plasma

    NASA Astrophysics Data System (ADS)

    Chrien, R. E.; Colestock, P. L.; Eubank, H. P.; Hosea, J. C.; Hwang, D. Q.; Strachan, J. D.; Thompson, H. R., Jr.

    1981-02-01

    d-3He reactions have been observed in the Princeton Large Torus by detecting the unconfined 14.7-MeV proton. Reaction rates as high as 2×1013 sec-1 resulting in 60 W of fusion power were obtained by 500-kW heating of a 3He minority in the ion-cyclotron range of frequencies to energies above 80 keV. The fusion-power multiplication of about 10-4 is equal to the highest obtained in any controlled-fusion experiment.

  5. Melting pressure of solid 3He in magnetic field near the nuclear ordering temperature

    NASA Astrophysics Data System (ADS)

    Sawada, A.; Shinozaki, A.; Itoh, W.; Torizuka, K.; Suga, M.; Satoh, T.; Komatsubara, T.

    1990-08-01

    The melting pressure of 3He was measured in magnetic field. The phase transitions of solid 3He were determined by the anomaly of the melting curves during the temperature and the magnetic field sweep. With thus determined B-T phase diagram, the slope of the transition line near the triple point suggests the first order transition of the paramagnetic to the high field phase transition. However, any trace of the latent heat was not observed within our experimental accuracy. The spin wave velocity in magnetic field was also obtained.

  6. Bounds on New Spin Dependent Forces Between Neutrons Using a ^3He / ^129Xe Zeeman Maser

    NASA Astrophysics Data System (ADS)

    Glenday, Alex; Cramer, Claire; Phillips, David F.; Walsworth, Ronald L.

    2008-05-01

    Searches for new spin dependent macroscopic forces place bounds on physics beyond the Standard Model, such as Lorentz symmetry violation and existence of new particles like the axion. We report the first experimental limits on new spin dependent macroscopic forces between neutron spins. We measure the nuclear Zeeman frequencies of a ^3He / ^129Xe maser while we modulate the nuclear spin polarization of ^3He in a separate glass cell. We place limits on the coupling strength of dipole potentials mediated by axion-like particles (gpgp) at the 5.5x10-6 level for interactions at ranges longer than 40 cm.

  7. Heat Transfer in 3{He}-4{He} Mixtures in Cylindrical Geometry

    NASA Astrophysics Data System (ADS)

    Nemchenko, K.; Rogova, S.; Vikhtinskaya, T.

    2017-05-01

    The paper presents the results of theoretical studies of the transport processes that take place in the newly proposed experiments on study of a vibrating quartz fork in superfluid 3{He}-4{He} mixtures. In addition to known mechanisms of energy loss from a vibrating quartz fork such as first sound radiation or interaction with thermal excitations, two more mechanisms specific for 3{He}-4{He} mixtures are proposed and studied in the paper. The relative contribution of these mechanisms: second sound and effective diffusion, is considered, and experimental conditions under which these mechanisms become effective are discussed.

  8. Polarising liquid [sup 3]He by fractional distillation: A competitive alternative

    SciTech Connect

    Vermeulen, G. )

    1994-01-01

    The method, pioneered by Nacher et al to produce polarized [sup 3]He by fractional distillation in a saturated [sup 3]He-[sup 4]He mixture at s.v.p. has been improved by using higher pressures. In principle, this method gives access to a non-equilibrium steady-state polarization in the concentrated as well as the dilute phase. The maximum polarization achieved in the dilute phase at a pressure of 10 bar, a temperature of 100-150 mK and an external field of 6.6 T is nearly 20%.

  9. Tricritical Casimir forces and order parameter profiles in wetting films of 3He-4He mixtures

    NASA Astrophysics Data System (ADS)

    Farahmand Bafi, N.; Maciołek, A.; Dietrich, S.

    2017-03-01

    Tricritical Casimir forces in 3He-4He wetting films are studied, within mean field theory, in terms of a suitable lattice gas model for binary liquid mixtures with short-ranged surface fields. The proposed model takes into account the continuous rotational symmetry O(2) of the superfluid degrees of freedom associated with 4He and it allows, inter alia, for the occurrence of a vapor phase. As a result, the model facilitates the formation of wetting films, which provides a strengthened theoretical framework to describe available experimental data for tricritical Casimir forces acting in 3He-4He wetting films.

  10. Relativistic, QED, and nuclear mass effects in the magnetic shielding of 3He.

    PubMed

    Rudziński, Adam; Puchalski, Mariusz; Pachucki, Krzysztof

    2009-06-28

    The magnetic shielding sigma of (3)He is studied. The complete relativistic corrections of order O(alpha(2)), leading QED corrections of order O(alpha(3) ln alpha), and finite nuclear mass effects of order O(m/m(N)) are calculated with high numerical precision. The resulting theoretical predictions for sigma = 59.967 43(10)x10(-6) are the most accurate to date among all elements and support the use of (3)He as a NMR standard.

  11. Heat Transfer in 3He -4He Mixtures in Cylindrical Geometry

    NASA Astrophysics Data System (ADS)

    Nemchenko, K.; Rogova, S.; Vikhtinskaya, T.

    2017-02-01

    The paper presents the results of theoretical studies of the transport processes that take place in the newly proposed experiments on study of a vibrating quartz fork in superfluid 3He -4He mixtures. In addition to known mechanisms of energy loss from a vibrating quartz fork such as first sound radiation or interaction with thermal excitations, two more mechanisms specific for 3He -4He mixtures are proposed and studied in the paper. The relative contribution of these mechanisms: second sound and effective diffusion, is considered, and experimental conditions under which these mechanisms become effective are discussed.

  12. Micro FT-IR Characterization Of Human Lung Tumor Cells

    NASA Astrophysics Data System (ADS)

    Benedetti, Enzo; Teodori, L.; Vergamini, Piergiorgio; Trinca, M. L.; Mauro, F.; Salvati, F.; Spremolla, Giuliano

    1989-12-01

    FT-IR spectroscopy has opened up a new approach to the analytical study of cell transformation. Investigations carried out in normal and leukemic lymphocytes have evidenced an increase in DNA with respect to proteic components in neoplastic cells.(1) The evaluation of the ratio of the integrated areas(A) of the bands at 1080 cm-1 (mainly DNA) and at 1540 cm-1 (proteic components) has allowed us to establish a parameter which indicates, for values above 1.5, the neoplastic nature of cells. Recently, this approach has been applied to the study of human lung tumor cells. Several monocellular suspension procedures of the tissue fragment (mechanical and/or chemical) were tested to obtain reproducible and reliable spectra able to differentiate clearly between normal and patological cells. Chemical treatment (EDTA, Pepsin, Collagenase, etc.) produced additional bands in the spectra of the cells causing distortion of the profiles of some absorptions, and as a result, mechanical treatment was preferred. The normal and neoplastic cells homogeneously distributed by cytospin preparation on BaF2 windows were examined by means of FT-IR microscopy. An examination of several microareas of each sample yielded reproducible spectra, with values of the A 1080 cm-1 / A 1540 cm-1 parameter within a very narrow range for each sample, even if certain differences still remained among the different cases, in good agreement with the results obtained for leukemic cells.(1) The value of this parameter was found to be lower for cells isolated from the normal area of lung, than in the case of those corresponding to the tumoral area, meaning that an increase occurs in DNA with respect to the proteic components. These insights, which provide a basis to obtain indications at the molecular level, can open up new possibilities in clinical practice, in order to obtain diagnosis confirmation, to detect early stages of disease and to offer additional indications in cases of dubious interpretation.

  13. Frizzled-8 as a putative therapeutic target in human lung cancer

    SciTech Connect

    Wang, Hua-qing; Xu, Mei-lin; Ma, Jie; Zhang, Yi; Xie, Cong-hua

    2012-01-06

    Highlights: Black-Right-Pointing-Pointer Fzd-8 is over-expressed in human lung cancer. Black-Right-Pointing-Pointer shRNA knock-down of Fzd-8 inhibits proliferation and Wnt pathway in lung cancer cells. Black-Right-Pointing-Pointer shRNA knock-down of Fzd-8 suppresses tumor growth in vivo. Black-Right-Pointing-Pointer shRNA knock-down Fzd-8 sensitizes lung cancer cells to chemotherapy Taxotere. -- Abstract: Lung cancer is the leading cause of cancer related deaths worldwide. It is necessary to better understand the molecular mechanisms involved in lung cancer in order to develop more effective therapeutics for the treatment of this disease. Recent reports have shown that Wnt signaling pathway is important in a number of cancer types including lung cancer. However, the role of Frizzled-8 (Fzd-8), one of the Frizzled family of receptors for the Wnt ligands, in lung cancer still remains to be elucidated. Here in this study we showed that Fzd-8 was over-expressed in human lung cancer tissue samples and cell lines. To investigate the functional importance of the Fzd-8 over-expression in lung cancer, we used shRNA to knock down Fzd-8 mRNA in lung cancer cells expressing the gene. We observed that Fzd-8 shRNA inhibited cell proliferation along with decreased activity of Wnt pathway in vitro, and also significantly suppressed A549 xenograft model in vivo (p < 0.05). Furthermore, we found that knocking down Fzd-8 by shRNA sensitized the lung cancer cells to chemotherapy Taxotere. These data suggest that Fzd-8 is a putative therapeutic target for human lung cancer and over-expression of Fzd-8 may be important for aberrant Wnt activation in lung cancer.

  14. Nanoparticle diffusion in respiratory mucus from humans without lung disease

    PubMed Central

    Schuster, Benjamin S.; Suk, Jung Soo; Woodworth, Graeme F.; Hanes, Justin

    2013-01-01

    A major role of respiratory mucus is to trap inhaled particles, including pathogens and environmental particulates, to limit body exposure. Despite the tremendous health implications, how particle size and surface chemistry affect mobility in respiratory mucus from humans without lung disease is not known. We prepared polymeric nanoparticles densely coated with low molecular weight polyethylene glycol (PEG) to minimize muco-adhesion, and compared their transport to that of uncoated particles in human respiratory mucus, which we collected from the endotracheal tubes of surgical patients with no respiratory comorbidities. We found that 100 and 200 nm diameter PEG-coated particles rapidly penetrated respiratory mucus, at rates exceeding their uncoated counterparts by approximately 15- and 35-fold, respectively. In contrast, PEG-coated particles ≥ 500 nm in diameter were sterically immobilized by the mucus mesh. Thus, even though respiratory mucus is a viscoelastic solid at the macroscopic level (as measured using a bulk rheometer), nanoparticles that are sufficiently small and muco-inert can penetrate the mucus as if it were primarily a viscous liquid. These findings help elucidate the barrier properties of respiratory mucus and provide design criteria for therapeutic nanoparticles capable of penetrating mucus to approach the underlying airway epithelium. PMID:23384790

  15. Three-dimensional scaffolds of acellular human and porcine lungs for high throughput studies of lung disease and regeneration.

    PubMed

    Wagner, Darcy E; Bonenfant, Nicholas R; Sokocevic, Dino; DeSarno, Michael J; Borg, Zachary D; Parsons, Charles S; Brooks, Elice M; Platz, Joseph J; Khalpey, Zain I; Hoganson, David M; Deng, Bin; Lam, Ying W; Oldinski, Rachael A; Ashikaga, Takamaru; Weiss, Daniel J

    2014-03-01

    Acellular scaffolds from complex whole organs such as lung are being increasingly studied for ex vivo organ generation and for in vitro studies of cell-extracellular matrix interactions. We have established effective methods for efficient de and recellularization of large animal and human lungs including techniques which allow multiple small segments (∼ 1-3 cm(3)) to be excised that retain 3-dimensional lung structure. Coupled with the use of a synthetic pleural coating, cells can be selectively physiologically inoculated via preserved vascular and airway conduits. Inoculated segments can be further sliced for high throughput studies. Further, we demonstrate thermography as a powerful noninvasive technique for monitoring perfusion decellularization and for evaluating preservation of vascular and airway networks following human and porcine lung decellularization. Collectively, these techniques are a significant step forward as they allow high throughput in vitro studies from a single lung or lobe in a more biologically relevant, three-dimensional acellular scaffold. Copyright © 2013 Elsevier Ltd. All rights reserved.

  16. The extracellular calcium-sensing receptor regulates human fetal lung development via CFTR.

    PubMed

    Brennan, Sarah C; Wilkinson, William J; Tseng, Hsiu-Er; Finney, Brenda; Monk, Bethan; Dibble, Holly; Quilliam, Samantha; Warburton, David; Galietta, Luis J; Kemp, Paul J; Riccardi, Daniela

    2016-02-25

    Optimal fetal lung growth requires anion-driven fluid secretion into the lumen of the developing organ. The fetus is hypercalcemic compared to the mother and here we show that in the developing human lung this hypercalcaemia acts on the extracellular calcium-sensing receptor, CaSR, to promote fluid-driven lung expansion through activation of the cystic fibrosis transmembrane conductance regulator, CFTR. Several chloride channels including TMEM16, bestrophin, CFTR, CLCN2 and CLCA1, are also expressed in the developing human fetal lung at gestational stages when CaSR expression is maximal. Measurements of Cl(-)-driven fluid secretion in organ explant cultures show that pharmacological CaSR activation by calcimimetics stimulates lung fluid secretion through CFTR, an effect which in humans, but not mice, was also mimicked by fetal hypercalcemic conditions, demonstrating that the physiological relevance of such a mechanism appears to be species-specific. Calcimimetics promote CFTR opening by activating adenylate cyclase and we show that Ca(2+)-stimulated type I adenylate cyclase is expressed in the developing human lung. Together, these observations suggest that physiological fetal hypercalcemia, acting on the CaSR, promotes human fetal lung development via cAMP-dependent opening of CFTR. Disturbances in this process would be expected to permanently impact lung structure and might predispose to certain postnatal respiratory diseases.

  17. Kinome sequencing reveals RET G691S polymorphism in human neuroendocrine lung cancer cell lines

    PubMed Central

    Sosonkina, Nadiya; Hong, Seung-Keun; Starenki, Dmytro; Park, Jong-In

    2014-01-01

    Neuroendocrine (NE) lung tumors comprise 20–25% of all invasive lung malignancies. Currently, no effective treatments are available to cure these tumors, and it is necessary to identify a molecular alteration(s) that characterizes NE lung tumor cells. We aimed to identify a kinase mutation(s) associated with NE lung tumor by screening 517 kinase-encoding genes in human lung cancer cell lines. Our next-generation sequencing analysis of six NE lung tumor cell lines (four small cell lung cancer lines and two non-small cell lung cancer lines) and three non-NE lung tumor lines revealed various kinase mutations, including a nonsynonymous mutation in the proto-oncogene RET (c.2071G>A; p.G691S). Further evaluation of the RET polymorphism in total 15 lung cancer cell lines by capillary sequencing suggested that the frequency of the minor allele (A-allele) in NE lung tumor lines was significantly higher than its frequency in a reference population (p = 0.0001). However, no significant difference between non-NE lung tumor lines and a reference group was detected (p = 1.0). Nevertheless, neither RET expression levels were correlated with the levels of neuron-specific enolase (NSE), a key NE marker, nor vandetanib and cabozantinib, small molecule compounds that inhibit RET, affected NSE levels in lung cancer cells. Our data suggest a potential association of G691S RET polymorphism with NE lung tumor, proposing the necessity of more thorough evaluation of this possibility. The dataset of kinase mutation profiles in this report may help choosing cell line models for study of lung cancer. PMID:25530832

  18. Application of a Neutral Community Model To Assess Structuring of the Human Lung Microbiome

    PubMed Central

    Venkataraman, Arvind; Bassis, Christine M.; Beck, James M.; Young, Vincent B.; Curtis, Jeffrey L.; Huffnagle, Gary B.

    2015-01-01

    ABSTRACT  DNA from phylogenetically diverse microbes is routinely recovered from healthy human lungs and used to define the lung microbiome. The proportion of this DNA originating from microbes adapted to the lungs, as opposed to microbes dispersing to the lungs from other body sites and the atmosphere, is not known. We use a neutral model of community ecology to distinguish members of the lung microbiome whose presence is consistent with dispersal from other body sites and those that deviate from the model, suggesting a competitive advantage to these microbes in the lungs. We find that the composition of the healthy lung microbiome is consistent with predictions of the neutral model, reflecting the overriding role of dispersal of microbes from the oral cavity in shaping the microbial community in healthy lungs. In contrast, the microbiome of diseased lungs was readily distinguished as being under active selection. We also assessed the viability of microbes from lung samples by cultivation with a variety of media and incubation conditions. Bacteria recovered by cultivation from healthy lungs represented species that comprised 61% of the 16S rRNA-encoding gene sequences derived from bronchoalveolar lavage samples. Importance  Neutral distribution of microbes is a distinguishing feature of the microbiome in healthy lungs, wherein constant dispersal of bacteria from the oral cavity overrides differential growth of bacteria. No bacterial species consistently deviated from the model predictions in healthy lungs, although representatives of many of the dispersed species were readily cultivated. In contrast, bacterial populations in diseased lungs were identified as being under active selection. Quantification of the relative importance of selection and neutral processes such as dispersal in shaping the healthy lung microbiome is a first step toward understanding its impacts on host health. PMID:25604788

  19. The Gas Motion Due To Non-Uniform Heating By 3He(n,p)3H Reactions In The Nuclear-Pumped3He -Lasers

    SciTech Connect

    Cetin, Fuesun

    2007-04-23

    In the nuclear pumped-lasers, the passage of these energetic charged particles through gas results in a non-uniform volumetric energy deposition. This spatial non-uniformity induces a gas motion, which results in density and hence refractive index gradients that affects the laser's optical behaviour. The motion of 3He gas in a closed cavity is studied when it experiences transient and spatially non-uniform volumetric heating caused by the passage of 3He(n,p)3H reaction products. Gas motion is described by the radial velocity field of gas flow. Spatial and temporal variations of radial gas velocity are calculated for various tube parameters by using a dynamic energy deposition model. In the calculations, it is assumed that the laser tube is irradiated with neutrons from the pulse at a peak power of 1200 MW corresponding to a maximum thermal neutron flux of 8x1016 n / cm2sn in the central channel of ITU TRIGA Mark II Reactor. Results are examined.

  20. Differential response of the epithelium and interstitium in developing human fetal lung explants to hyperoxia.

    PubMed

    Bustani, Porus; Hodge, Rachel; Tellabati, Ananth; Li, Juan; Pandya, Hitesh; Kotecha, Sailesh

    2006-03-01

    Hyperoxia is closely linked with the development of chronic lung disease of prematurity (CLD), but the exact mechanisms whereby hyperoxia alters the lung architecture in the developing lung remain largely unknown. We developed a fetal human lung organ culture model to investigate (a) the morphologic changes induced by hyperoxia and (b) whether hyperoxia resulted in differential cellular responses in the epithelium and interstitium. The effects of hyperoxia on lung morphometry were analyzed using computer-assisted image analysis. The lung architecture remained largely unchanged in normoxia lasting as long as 4 d. In contrast, hyperoxic culture of pseudoglandular fetal lungs resulted in significant dilatation of airways, thinning of the epithelium, and regression of the interstitium including the pulmonary vasculature. Although there were no significant differences in Ki67 between normoxic and hyperoxic lungs, activated caspase-3 was significantly increased in interstitial cells, but not epithelial cells, under hyperoxic conditions. These changes show that exposure of pseudoglandular lungs to hyperoxia modulates the lung architecture to resemble saccular lungs.

  1. In vitro immunological degranulation of human basophils is modulated by lung histamine and Apis mellifica.

    PubMed Central

    Poitevin, B; Davenas, E; Benveniste, J

    1988-01-01

    1. The effect of high dilutions of two homeopathic drugs Lung histamine (Lung his) and Apis mellifica (Apis mel) used for the treatment of allergic diseases has been assessed on in vitro human basophil degranulation. Experiments were conducted blind. 2. Basophil degranulation induced by 1.66 X 10(-9) M anti-IgE antibody was significantly inhibited in the presence of 5 Lung his (5th centesimal dilution of Lung his) and 15 Lung his (15th centesimal dilution of Lung his) by 28.8% and 28.6% respectively and by 65.8% in the presence of 9 Apis mel (9th centesimal dilution of Apis mel). Basophil degranulation induced by 1.66 X 10(-16) to 1.66 X 10(-18) M anti-IgE antibody was also inhibited by high dilutions of Lung his and Apis mel with an inhibition of nearly 100% with 18 Lung his (18th centesimal dilution of Lung his) and 10 Apis mel (10th centesimal dilution of Apis mel). An alternance of inhibition, inactivity and stimulation was observed when basophils were incubated in the presence of serial dilutions of Lung his and Apis mel. 3. The investigation of the clinical efficacy of high dilutions of Lung his and Apis mel should be envisaged in allergic diseases in parallel with in vitro and ex vivo biological assays. PMID:3382588

  2. Lung Beractant Increases Free Cytosolic Levels of Ca2+ in Human Lung Fibroblasts

    PubMed Central

    Guzmán-Silva, Alejandro; Vázquez de Lara, Luis G.; Torres-Jácome, Julián; Vargaz-Guadarrama, Ajelet; Flores-Flores, Marycruz; Pezzat Said, Elias; Lagunas-Martínez, Alfredo; Mendoza-Milla, Criselda; Tanzi, Franco; Moccia, Francesco; Berra-Romani, Roberto

    2015-01-01

    Beractant, a natural surfactant, induces an antifibrogenic phenotype and apoptosis in normal human lung fibroblasts (NHLF). As intracellular Ca2+ signalling has been related to programmed cell death, we aimed to assess the effect of beractant on intracellular Ca2+ concentration ([Ca2+]i) in NHLF in vitro. Cultured NHLF were loaded with Fura-2 AM (3 μM) and Ca2+ signals were recorded by microfluorimetric techniques. Beractant causes a concentration-dependent increase in [Ca2+]i with a EC50 of 0.82 μg/ml. The application of beractant, at a concentration of 500 μg/ml, which has been shown to exert an apoptotic effect in human fibroblasts, elicited different patterns of Ca2+ signals in NHLF: a) a single Ca2+ spike which could be followed by b) Ca2+ oscillations, c) a sustained Ca2+ plateau or d) a sustained plateau overlapped by Ca2+ oscillations. The amplitude and pattern of Ca2+ transients evoked by beractant were dependent on the resting [Ca2+]i. Pharmacological manipulation revealed that beractant activates a Ca2+ signal through Ca2+ release from intracellular stores mediated by phospholipase Cβ (PLCβ), Ca2+ release from inositol 1,4,5-trisphosphate receptors (IP3Rs) and Ca2+ influx via a store-operated pathway. Moreover, beractant-induced Ca2+ release was abolished by preventing membrane depolarization upon removal of extracellular Na+ and Ca2+. Finally, the inhibition of store-operated channels prevented beractant-induced NHLF apoptosis and downregulation of α1(I) procollagen expression. Therefore, beractant utilizes SOCE to exert its pro-apoptotic and antifibrinogenic effect on NHLF. PMID:26230503

  3. CD11b immunophenotyping identifies inflammatory profiles in the mouse and human lungs.

    PubMed

    Duan, M; Steinfort, D P; Smallwood, D; Hew, M; Chen, W; Ernst, M; Irving, L B; Anderson, G P; Hibbs, M L

    2016-03-01

    The development of easily accessible tools for human immunophenotyping to classify patients into discrete disease endotypes is advancing personalized therapy. However, no systematic approach has been developed for the study of inflammatory lung diseases with often complex and highly heterogeneous disease etiologies. We have devised an internally standardized flow cytometry approach that can identify parallel inflammatory alveolar macrophage phenotypes in both the mouse and human lungs. In mice, lung innate immune cell alterations during endotoxin challenge, influenza virus infection, and in two genetic models of chronic obstructive lung disease could be segregated based on the presence or absence of CD11b alveolar macrophage upregulation and lung eosinophilia. Additionally, heightened alveolar macrophage CD11b expression was a novel feature of acute lung exacerbations in the SHIP-1(-/-) model of chronic obstructive lung disease, and anti-CD11b antibody administration selectively blocked inflammatory CD11b(pos) but not homeostatic CD11b(neg) alveolar macrophages in vivo. The identification of analogous profiles in respiratory disease patients highlights this approach as a translational avenue for lung disease endotyping and suggests that heterogeneous innate immune cell phenotypes are an underappreciated component of the human lung disease microenvironment.

  4. Evidence against a role for jaagsiekte sheep retrovirus in human lung cancer.

    PubMed

    Miller, A Dusty; De Las Heras, Marcelo; Yu, Jingyou; Zhang, Fushun; Liu, Shan-Lu; Vaughan, Andrew E; Vaughan, Thomas L; Rosadio, Raul; Rocca, Stefano; Palmieri, Giuseppe; Goedert, James J; Fujimoto, Junya; Wistuba, Ignacio I

    2017-01-20

    Jaagsiekte sheep retrovirus (JSRV) causes a contagious lung cancer in sheep and goats that can be transmitted by aerosols produced by infected animals. Virus entry into cells is initiated by binding of the viral envelope (Env) protein to a specific cell-surface receptor, Hyal2. Unlike almost all other retroviruses, the JSRV Env protein is also a potent oncoprotein and is responsible for lung cancer in animals. Of concern, Hyal2 is a functional receptor for JSRV in humans. We show here that JSRV is fully capable of infecting human cells, as measured by its reverse transcription and persistence in the DNA of cultured human cells. Several studies have indicated a role for JSRV in human lung cancer while other studies dispute these results. To further investigate the role of JSRV in human lung cancer, we used highly-specific mouse monoclonal antibodies and a rabbit polyclonal antiserum against JSRV Env to test for JSRV expression in human lung cancer. JSRV Env expression was undetectable in lung cancers from 128 human subjects, including 73 cases of bronchioalveolar carcinoma (BAC; currently reclassified as lung invasive adenocarcinoma with a predominant lepidic component), a lung cancer with histology similar to that found in JSRV-infected sheep. The BAC samples included 8 JSRV DNA-positive samples from subjects residing in Sardinia, Italy, where sheep farming is prevalent and JSRV is present. We also tested for neutralizing antibodies in sera from 138 Peruvians living in an area where sheep farming is prevalent and JSRV is present, 24 of whom were directly exposed to sheep, and found none. We conclude that while JSRV can infect human cells, JSRV plays little if any role in human lung cancer.

  5. Degassing of 3H/3He, CFCs and SF6 by denitrification: measurements and two-phase transport simulations.

    PubMed

    Visser, Ate; Schaap, Joris D; Broers, Hans Peter; Bierkens, Marc F P

    2009-01-26

    The production of N2 gas by denitrification may lead to the appearance of a gas phase below the water table prohibiting the conservative transport of tracer gases required for groundwater dating. We used a two-phase flow and transport model (STOMP) to study the reliability of 3H/3He, CFCs and SF6 as groundwater age tracers under agricultural land where denitrification causes degassing. We were able to reproduce the amount of degassing (R2=69%), as well as the 3H (R2=79%) and 3He (R2=76%) concentrations observed in a 3H/3He data set using simple 2D models. We found that the TDG correction of the 3H/3He age overestimated the control 3He/3He age by 2.1 years, due to the accumulation of 3He in the gas phase. The total uncertainty of degassed 3H/3He ages of 6 years (+/-2 sigma) is due to the correction of degassed 3He using the TDG method, but also due to the travel time in the unsaturated zone and the diffusion of bomb peak 3He. CFCs appear to be subject to significant degradation in anoxic groundwater and SF6 is highly susceptible to degassing. We conclude that 3H/3He is the most reliable method to date degassed groundwater and that two-phase flow models such as STOMP are useful tools to assist in the interpretation of degassed groundwater age tracer data.

  6. Degassing of 3H/ 3He, CFCs and SF 6 by denitrification: Measurements and two-phase transport simulations

    NASA Astrophysics Data System (ADS)

    Visser, Ate; Schaap, Joris D.; Broers, Hans Peter; Bierkens, Marc F. P.

    2009-01-01

    The production of N 2 gas by denitrification may lead to the appearance of a gas phase below the water table prohibiting the conservative transport of tracer gases required for groundwater dating. We used a two-phase flow and transport model (STOMP) to study the reliability of 3H/ 3He, CFCs and SF 6 as groundwater age tracers under agricultural land where denitrification causes degassing. We were able to reproduce the amount of degassing ( R2 = 69%), as well as the 3H ( R2 = 79%) and 3He* ( R2 = 76%) concentrations observed in a 3H/ 3He data set using simple 2D models. We found that the TDG correction of the 3H/ 3He age overestimated the control 3He/ 3He age by 2.1 years, due to the accumulation of 3He* in the gas phase. The total uncertainty of degassed 3H/ 3He ages of 6 years (± 2 σ) is due to the correction of degassed 3He* using the TDG method, but also due to the travel time in the unsaturated zone and the diffusion of bomb peak 3He*. CFCs appear to be subject to significant degradation in anoxic groundwater and SF 6 is highly susceptible to degassing. We conclude that 3H/ 3He is the most reliable method to date degassed groundwater and that two-phase flow models such as STOMP are useful tools to assist in the interpretation of degassed groundwater age tracer data.

  7. Surface Majorana fermions and bulk collective modes in superfluid 3He-B

    NASA Astrophysics Data System (ADS)

    Park, YeJe; Chung, Suk Bum; Maciejko, Joseph

    2015-02-01

    The theoretical study of topological superfluids and superconductors has so far been carried out largely as a translation of the theory of noninteracting topological insulators into the superfluid language, whereby one replaces electrons by Bogoliubov quasiparticles and single-particle band Hamiltonians by Bogoliubov-de Gennes Hamiltonians. Band insulators and superfluids are, however, fundamentally different: While the former exist in the absence of interparticle interactions, the latter are broken symmetry states that owe their very existence to such interactions. In particular, unlike the static energy gap of a band insulator, the gap in a superfluid is due to a dynamical order parameter that is subject to both thermal and quantum fluctuations. In this work, we explore the consequences of bulk quantum fluctuations of the order parameter in the B phase of superfluid 3He on the topologically protected Majorana surface states. Neglecting the high-energy amplitude modes, we find that one of the three spin-orbit Goldstone modes in 3He-B couples to the surface Majorana fermions. This coupling in turn induces an effective short-range two-body interaction between the Majorana fermions, with coupling constant inversely proportional to the strength of the nuclear dipole-dipole interaction in bulk 3He. A mean-field theory suggests that the surface Majorana fermions in 3He-B may be in the vicinity of a metastable gapped time-reversal-symmetry-breaking phase.

  8. Torsion Pendulum Experiments with Superfluid 3He in ``Nematically Ordered'' Aerogel

    NASA Astrophysics Data System (ADS)

    Zhelev, Nikolay; Smith, Eric; Sebastian, Abhilash; Parpia, Jeevak

    2014-03-01

    A new type of highly anisotropic alumina aerogel is used to induce directional disorder in superfluid 3He. The aerogel sample consists of a network of long strands that have a preferred orientation (nematic order). It is placed in the head of a double torsion pendulum with the anisotropy axis oriented along the axis of the pendulum. We observe the frequency shift of the symmetric torsion mode of the pendulum in order to determine the superfluid fraction of the embedded 3He. The superfluid transition temperature of the fluid in the aerogel is measured to be very close to that of bulk 3He. However, in contrast to the bulk phase diagram, the region of stability of the Equal Spin Pairing (ESP) superfluid phase is enhanced on cooling. In addition, unlike the case of 3He in isotropic silica aerogel, the ESP phase reappears on warming. We compare our measurements to the NMR data reported in and discuss the possible structure of the observed superfluid phases.

  9. Effect of 3He on the extinction of mass flux in solid helium

    NASA Astrophysics Data System (ADS)

    Vekhov, Ye.; Hallock, Robet

    2014-03-01

    The flux, F, carried by solid 4He , with nominal 300 ppb 3He concentration, χ, in the range 25.6 - 26.3 bar rises with falling temperature and at a temperature Td the flux decreases toward zero. The behavior of the flux above Td demonstrates the presence of a bosonic Luttinger liquid. We study F as a function of 3He concentration χ to explore the effect of 3He on Td. We find that the extinction of the flux is a sharp transition, typically complete within a few mK change in temperature. We find that Td is an increasing function of χ and we compare (Td , χ) with predictions for homogeneous phase separation. We conclude that phase separation plays an important role in the flux extinction. It is possible that the cores of edge dislocations carry the flux, and the flux is extinguished by the decoration by 3He of the cores or dislocation intersections. Supported by NSF DMR 12-05217.

  10. Spin correlations in quasi-elastic electron scattering from a (3)He internal target

    NASA Astrophysics Data System (ADS)

    Six, R. Edward, III

    The measurement of spin observables in the 3He-> (e->,e' ,d) and 3He-> (e->,e' ,p) reactions have been carried out at the Internal Target Facility of the Dutch National Institute for Nuclear and High Energy Physics (NIKHEF) in Amsterdam, The Netherlands, with a 720-MeV stored electron beam having a longitudinal polarization of 65% and an average current of 80 mA. This was the first measurement of the spin correlation parameters for the reaction 3He-> (e->,e' ,d) . The average target polarization was 45% with a thickness of 5 × 1014 atoms/cm2. The scattered electrons were detected in a large-acceptance, nonfocusing magnetic spectrometer located at a central angle of 40°. The knockout hadrons were detected in a non-magnetic detector located at a central angle of -56°. The central positions of the detectors correspond to quasi-elastic kinematics. The asymmetries A'x and A'x provide information on small components of the 3He ground-state wave function and on the isoscalar/isovector structure of the nuclear electromagnetic current. The results are compared with model calculations.

  11. Deep mixing of 3He: reconciling Big Bang and stellar nucleosynthesis.

    PubMed

    Eggleton, Peter P; Dearborn, David S P; Lattanzio, John C

    2006-12-08

    Low-mass stars, approximately 1 to 2 solar masses, near the Main Sequence are efficient at producing the helium isotope 3He, which they mix into the convective envelope on the giant branch and should distribute into the Galaxy by way of envelope loss. This process is so efficient that it is difficult to reconcile the low observed cosmic abundance of 3He with the predictions of both stellar and Big Bang nucleosynthesis. Here we find, by modeling a red giant with a fully three-dimensional hydrodynamic code and a full nucleosynthetic network, that mixing arises in the supposedly stable and radiative zone between the hydrogen-burning shell and the base of the convective envelope. This mixing is due to Rayleigh-Taylor instability within a zone just above the hydrogen-burning shell, where a nuclear reaction lowers the mean molecular weight slightly. Thus, we are able to remove the threat that 3He production in low-mass stars poses to the Big Bang nucleosynthesis of 3He.

  12. Deep Mixing of 3He: Reconciling Big Bang and Stellar Nucleosynthesis

    SciTech Connect

    Eggleton, P P; Dearborn, D P; Lattanzio, J

    2006-07-26

    Low-mass stars, {approx} 1-2 solar masses, near the Main Sequence are efficient at producing {sup 3}He, which they mix into the convective envelope on the giant branch and should distribute into the Galaxy by way of envelope loss. This process is so efficient that it is difficult to reconcile the low observed cosmic abundance of {sup 3}He with the predictions of both stellar and Big Bang nucleosynthesis. In this paper we find, by modeling a red giant with a fully three-dimensional hydrodynamic code and a full nucleosynthetic network, that mixing arises in the supposedly stable and radiative zone between the hydrogen-burning shell and the base of the convective envelope. This mixing is due to Rayleigh-Taylor instability within a zone just above the hydrogen-burning shell, where a nuclear reaction lowers the mean molecular weight slightly. Thus we are able to remove the threat that {sup 3}He production in low-mass stars poses to the Big Bang nucleosynthesis of {sup 3}He.

  13. Zeeman relaxation of cold atomic iron and nickel in collisions with {sup 3}He

    SciTech Connect

    Johnson, Cort; Newman, Bonna; Kleppner, Daniel; Greytak, Thomas J.; Brahms, Nathan; Doyle, John M.

    2010-06-15

    We have measured the ratio {gamma} of the diffusion cross section to the angular momentum reorientation cross section in the colliding Fe-{sup 3}He and Ni-{sup 3}He systems. Nickel (Ni) and iron (Fe) atoms are introduced via laser ablation into a cryogenically cooled experimental cell containing cold (<1 K) {sup 3}He buffer gas. Elastic collisions rapidly cool the translational temperature of the ablated atoms to the {sup 3}He temperature. {gamma} is extracted by measuring the decays of the atomic Zeeman sublevels. For our experimental conditions, thermal energy is comparable to the Zeeman splitting. As a result, thermal excitations between Zeeman sublevels significantly impact the observed decay. To determine {gamma} accurately, we introduce a model of Zeeman-state dynamics that includes thermal excitations. We find {gamma}{sub Ni-}{sup 3}{sub He}=5x10{sup 3} and {gamma}{sub Fe-}{sup 3}{sub He{<=}}3x10{sup 3} at 0.75 K in a 0.8-T magnetic field. These measurements are interpreted in the context of submerged shell suppression of spin relaxation, as studied previously in transition metals and rare-earth-metal atoms [C. I. Hancox, S. C. Doret, M. T. Hummon, R. V. Krems, and J. M. Doyle, Phys. Rev. Lett. 94, 013201 (2005); C. I. Hancox, S. C. Doret, M. T. Hummon, L. Luo, and J. M. Doyle, Nature (London) 431, 281 (2004); A. Buchachenko, G. Chaasiski, and M. Szczniak, Eur. Phys. J. D 45, 147 (2007)].

  14. Testing on novel neutron detectors as alternative to 3He for security applications

    NASA Astrophysics Data System (ADS)

    Peerani, Paolo; Tomanin, Alice; Pozzi, Sara; Dolan, Jennifer; Miller, Eric; Flaska, Marek; Battaglieri, Marco; De Vita, Raffaella; Ficini, Luisa; Ottonello, Giacomo; Ricco, Giovanni; Dermody, Geraint; Giles, Calvin

    2012-12-01

    Detection of illicit trafficking of nuclear material relies on the detection of the radiation emitted. In the case of plutonium, one of the characteristic signatures derives from neutron emission. For this reason, neutron detectors cover an important role in detection systems. Most current neutron detection systems used for nuclear security are based on the 3He technology. Unfortunately, in the last few years the market of 3He has encountered huge problems in matching the supply and the demand. The need has grown significantly due to the increasing demand of instrumentation for security. This has caused an exponential increase of the price from one side and on the other side a serious strategic problem of resources. In order to guarantee the availability of detection systems for nuclear security, it is necessary to develop alternative detection systems based on technologies different from 3He. Many research projects have been devoted for the development of novel neutron detectors both by research organisations and by industries. Scientists from the PERLA laboratory of the Joint Research Centre (JRC) in Ispra, Italy, and their collaborators have tested several of these novel concepts in the last couple of years. This paper describes the detector systems tested at JRC and preliminary results on detectors that can be considered as promising alternatives to 3He.

  15. A New Method for Precision Cold Neutron Polarimetry Using a (3)He Spin Filter.

    PubMed

    Wietfeldt, F E; Gentile, T R

    2005-01-01

    We present a new method for precision measurement of the capture flux polarization of a polychromatic (white), continuous cold neutron beam, polarized by a (3)He spin filter. This method allows an in situ measurement and does not require knowledge of the neutron beam wavelength distribution. We show that a polarimetry precision of 0.1 % is possible.

  16. Packed Powder as Superleak for Spin Pump Experiments in Superfluid 3He A1

    NASA Astrophysics Data System (ADS)

    Kamada, N.; Yamaguchi, A.; Motoyama, G.; Sumiyama, A.; Aoki, Y.; Okuda, Y.; Kojima, H.

    2014-04-01

    Experimental exploration of highly spin-polarized states of liquid 3He by applying external magnetic field is limited by the availability of static magnetic field. In the "ferromagnetic" superfluid A1 phase of liquid 3He there is an alternate method for boosting spin-polarization by the process of spin pumping without requiring such high magnetic field. The spin pumping in the A1 phase takes advantage of a superleak (SL) acting simultaneously as a filter for both entropy and spin. The spin pump technique that uses the SL-spin filter and a mechanical actuator enables us to directly boost polarization of 3He. The amount of enhancement of spin polarization has been limited so far. We are now developing a new type of SL filter made of packed aluminum oxide powder (referred as PAP-SL), in order to achieve greater enhancement of spin polarization. Several kinds of the PAP-SL filter were constructed by pressing aluminum oxide powders into a cylinder holder. The packed structures were carefully characterized by a flow-rate-measurement, X-ray tomography, and mercury intrusion porosimetry. The preliminary result shows that the PAP-SL works as SL filter for the superfluid 3He.

  17. Direct Observation of a Majorana Quasiparticle Heat Capacity in 3He

    NASA Astrophysics Data System (ADS)

    Bunkov, Y. M.

    2014-04-01

    The Majorana fermion, which acts as its own antiparticle, was suggested by Majorana in 1937 (Nuovo Cimento 14:171). While no stable particle with Majorana properties has yet been observed, Majorana quasiparticles (QP) may exist at the boundaries of topological insulators. Here we report the preliminary results of direct observation of Majorana QPs by a precise measurements of superfluid 3He heat capacity. The bulk superfluid 3He heat capacity falls exponentially with cooling at the temperatures significantly below the energy gap. Owing to the zero energy gap mode the Majorana heat capacity falls in a power law. The Majorana heat capacity can be larger than bulk one at some temperature, which depends on surface to volume ratio of the experimental cell. Some times ago we developed the Dark matter particles detector (DMD) on a basis of superfluid 3He which is working at the frontier of extremely low temperatures (Winkelmann et al., Nucl. Instrum. Meth. A 559:384-386, 2006). Here we report the observation of zero gap mode of Majorana, follows from the new analyses of DMD heat capacity, published early. We have found a 10 % deviation from the bulk superfluid 3He heat capacity at the temperature of 135 μK. This deviation corresponds well to the theoretical value for Majorana heat capacity at such low temperature. (Note, there were no fitting parameters).

  18. Scattering of 30 MeV {sup 3}He from {sup 185}Re

    SciTech Connect

    Garrett, P. E.; Phillips, A. A.; Demand, G. A.; Finlay, P.; Green, K. L.; Leach, K. G.; Schumaker, M. A.; Svensson, C. E.; Wong, J.; Hertenberger, R.; Wirth, H.-F.; Faestermann, T.; Kruecken, R.; Burke, D. G.; Bettermann, L.; Braun, N.

    2009-01-15

    The scattering of 30 MeV {sup 3}He from a {sup 185}Re target has been investigated. The measured elastic scattering is in disagreement with calculations using common optical model parameter sets found in the literature. A new optical model parameter set has been determined that reproduces the data for both the elastic and the inelastic scattering channels.

  19. First viscosity of dilute3He-4He mixtures below 0.6 K

    NASA Astrophysics Data System (ADS)

    Um, Chung-In; Yoo, Sahng-Kyoon; Lee, Soo-Young; George, Thomas F.; Pandey, Lakshmi N.

    1994-01-01

    Starting with the Boltzmann transport equation, the first viscosity of dilute3He-4He mixtures for various3He concentrations x is evaluated up to around T ≅ 0.6 K by including the contribution from three-phonon processes (3PP) in the anomalous elementary excitation spectrum of liquid4He. Due to 3PP, the characteristic time τη for3He viscosity at high temperatures, i.e., T⩾2TF where TF is the3He Fermi temperature, is evaluated as 5 × 10-12/xT, which is smaller than the value estimated by Rosenbaum et al. This is interpolated with τη in the degenerate (quantum) region, T≪TF. The obtained viscosities are in better agreement with experimental results than those of Baym and Saam, whose theory does not include 3PP. However, at very low concentrations there exists a discrepancy between the present theory and experiments, so that an alternate treatment should be considered.

  20. Precise /sup 3/H-/sup 3/He mass difference for neutrino mass determination

    SciTech Connect

    Lippmaa, E.; Pikver, R.; Suurmaa, E.; Past, J.; Puskar, J.; Koppel, I.; Tammik, A.

    1985-01-28

    The precise /sup 3/H-/sup 3/He atomic mass difference has been measured by high-resolution (10/sup -8/) ion cyclotron resonance in a 4.7-T magnetic field. The result of 18 599 +- 2 eV favors a nonzero electron antineutrino mass.

  1. Beam suppression of the DRAGON recoil separator for 3He(α,γ)7Be

    NASA Astrophysics Data System (ADS)

    Sjue, S. K. L.; Nara Singh, B. S.; Adsley, P.; Buchmann, L.; Carmona-Gallardo, M.; Davids, B.; Fallis, J.; Fulton, B. R.; Galinski, N.; Hager, U.; Hass, M.; Howell, D.; Hutcheon, D. A.; Laird, A. M.; Martin, L.; Ottewell, D.; Reeve, S.; Ruiz, C.; Ruprecht, G.; Triambak, S.

    2013-02-01

    Preliminary studies in preparation for an absolute cross-section measurement of the radiative capture reaction 3He(α,γ)7Be with the DRAGON recoil separator have demonstrated beam suppression >1014 at the 90% confidence level. A measurement of this cross section by observation of 7Be recoils at the focal plane of the separator should be virtually background free.

  2. Developments of In-Situ SEOP Polarized 3He Neutron Spin Filter in Japan

    NASA Astrophysics Data System (ADS)

    Kira, H.; Sakaguchi, Y.; Oku, T.; Suzuki, J.; Nakamura, M.; Arai, M.; Endoh, Y.; Chang, L. J.; Kakurai, K.; Arimoto, Y.; Ino, T.; Shimizu, H. M.; Kamiyama, T.; Ohoyama, K.; Hiraka, H.; Tsutsumi, K.; Yamada, K.

    2011-06-01

    We launched the polarized 3He neutron spin filters (NSF) project in order to provide neutron polarization for the pulsed neutron beams in Japan. We adopted the in-situ spin exchange optical pumping (SEOP) technique to polarize the nuclear spin of 3He atoms because it has some advantages for our applications. The overall system size is compact and it avoids the problem of the time decay of nuclear spin of 3He thus suppressing the costs of maintenance and providing other advantages [1, 2] with respect to data analysis and quality. In this paper, we performed pulsed neutron beam tests of our compact in-situ SEOP NSF system at the BL10 beamline in the Materials and Life Science Experimental Facility of the Japan Proton Accelerator Research Complex (J-PARC). The polarization of the 3He gas reached was 73 % and a pump-up time constant of 9.5 h was observed. This paper is a status report about the development of in-situ SEOP NSF system for the pulsed beam at J-PARC.

  3. Energy spectra of 3He-rich solar energetic particles associated with coronal waves

    NASA Astrophysics Data System (ADS)

    Bučík, R.; Innes, D. E.; Mason, G. M.; Wiedenbeck, M. E.

    2016-11-01

    In addition to their anomalous abundances, 3He-rich solar energetic particles (SEPs) show puzzling energy spectral shapes varying from rounded forms to power laws where the later are characteristics of shock acceleration. Solar sources of these particles have been often associated with jets and narrow CMEs, which are the signatures of magnetic reconnection involving open field. Recent reports on new associations with large-scale EUV waves bring new insights on acceleration and transport of 3He-rich SEPs in the corona. We examined energy spectra for 32 3He-rich SEP events observed by ACE at L1 near solar minimum in 2007-2010 and compared the spectral shapes with solar flare signatures obtained from STEREO EUV images. We found the events with jets or brightenings tend to be associated with rounded spectra and the ev