Temporal bone bank: complying with European Union directives on human tissue and cells.

PubMed

Van Rompaey, Vincent; Vandamme, Wouter; Muylle, Ludo; Van de Heyning, Paul H

2012-06-01

Availability of allograft tympano-ossicular systems (ATOS) provides unique reconstructive capabilities, allowing more radical removal of middle ear pathology. To provide ATOS, the University of Antwerp Temporal Bone Bank (UATB) was established in 1988. ATOS use was stopped in many countries because of safety issues concerning human tissue transplantation. Our objective was to maintain an ATOS tissue bank complying with European Union (EU) directives on human tissues and cells. The guidelines of the Belgian Superior Health Council, including EU directive requirements, were rigorously applied to UATB infrastructure, workflow protocols and activity. Workflow protocols were updated and an internal audit was performed to check and improve consistency with established quality systems and changing legislations. The Belgian Federal Agency of Medicines and Health Products performed an inspection to examine compliance with national legislatives and EU directives on human tissues and cells. A sample of important procedures was meticulously examined in its workflow setting next to assessment of the infrastructure and personnel. Results are reported on infrastructure, personnel, administrative workflow, procurement, preparation, processing, distribution, internal audit and inspection by the competent authority. Donors procured: 2006, 93 (45.1%); 2007, 64 (20.6%); 2008, 56 (13.1%); 2009, 79 (6.9%). The UATB was approved by the Minister of Health without critical or important shortcomings. The Ministry accords registration each time for 2 years. An ATOS tissue bank complying with EU regulations on human allografts is feasible and critical to assure that the patient receives tissue, which is safe, individually checked and prepared in a suitable environment.

BPELPower—A BPEL execution engine for geospatial web services

NASA Astrophysics Data System (ADS)

Yu, Genong (Eugene); Zhao, Peisheng; Di, Liping; Chen, Aijun; Deng, Meixia; Bai, Yuqi

2012-10-01

The Business Process Execution Language (BPEL) has become a popular choice for orchestrating and executing workflows in the Web environment. As one special kind of scientific workflow, geospatial Web processing workflows are data-intensive, deal with complex structures in data and geographic features, and execute automatically with limited human intervention. To enable the proper execution and coordination of geospatial workflows, a specially enhanced BPEL execution engine is required. BPELPower was designed, developed, and implemented as a generic BPEL execution engine with enhancements for executing geospatial workflows. The enhancements are especially in its capabilities in handling Geography Markup Language (GML) and standard geospatial Web services, such as the Web Processing Service (WPS) and the Web Feature Service (WFS). BPELPower has been used in several demonstrations over the decade. Two scenarios were discussed in detail to demonstrate the capabilities of BPELPower. That study showed a standard-compliant, Web-based approach for properly supporting geospatial processing, with the only enhancement at the implementation level. Pattern-based evaluation and performance improvement of the engine are discussed: BPELPower directly supports 22 workflow control patterns and 17 workflow data patterns. In the future, the engine will be enhanced with high performance parallel processing and broad Web paradigms.

3D correlative light and electron microscopy of cultured cells using serial blockface scanning electron microscopy

PubMed Central

Lerner, Thomas R.; Burden, Jemima J.; Nkwe, David O.; Pelchen-Matthews, Annegret; Domart, Marie-Charlotte; Durgan, Joanne; Weston, Anne; Jones, Martin L.; Peddie, Christopher J.; Carzaniga, Raffaella; Florey, Oliver; Marsh, Mark; Gutierrez, Maximiliano G.

2017-01-01

ABSTRACT The processes of life take place in multiple dimensions, but imaging these processes in even three dimensions is challenging. Here, we describe a workflow for 3D correlative light and electron microscopy (CLEM) of cell monolayers using fluorescence microscopy to identify and follow biological events, combined with serial blockface scanning electron microscopy to analyse the underlying ultrastructure. The workflow encompasses all steps from cell culture to sample processing, imaging strategy, and 3D image processing and analysis. We demonstrate successful application of the workflow to three studies, each aiming to better understand complex and dynamic biological processes, including bacterial and viral infections of cultured cells and formation of entotic cell-in-cell structures commonly observed in tumours. Our workflow revealed new insight into the replicative niche of Mycobacterium tuberculosis in primary human lymphatic endothelial cells, HIV-1 in human monocyte-derived macrophages, and the composition of the entotic vacuole. The broad application of this 3D CLEM technique will make it a useful addition to the correlative imaging toolbox for biomedical research. PMID:27445312

Scrambled eggs: A highly sensitive molecular diagnostic workflow for Fasciola species specific detection from faecal samples

PubMed Central

Calvani, Nichola Eliza Davies; Windsor, Peter Andrew; Bush, Russell David

2017-01-01

Background Fasciolosis, due to Fasciola hepatica and Fasciola gigantica, is a re-emerging zoonotic parasitic disease of worldwide importance. Human and animal infections are commonly diagnosed by the traditional sedimentation and faecal egg-counting technique. However, this technique is time-consuming and prone to sensitivity errors when a large number of samples must be processed or if the operator lacks sufficient experience. Additionally, diagnosis can only be made once the 12-week pre-patent period has passed. Recently, a commercially available coprological antigen ELISA has enabled detection of F. hepatica prior to the completion of the pre-patent period, providing earlier diagnosis and increased throughput, although species differentiation is not possible in areas of parasite sympatry. Real-time PCR offers the combined benefits of highly sensitive species differentiation for medium to large sample sizes. However, no molecular diagnostic workflow currently exists for the identification of Fasciola spp. in faecal samples. Methodology/Principal findings A new molecular diagnostic workflow for the highly-sensitive detection and quantification of Fasciola spp. in faecal samples was developed. The technique involves sedimenting and pelleting the samples prior to DNA isolation in order to concentrate the eggs, followed by disruption by bead-beating in a benchtop homogeniser to ensure access to DNA. Although both the new molecular workflow and the traditional sedimentation technique were sensitive and specific, the new molecular workflow enabled faster sample throughput in medium to large epidemiological studies, and provided the additional benefit of speciation. Further, good correlation (R2 = 0.74–0.76) was observed between the real-time PCR values and the faecal egg count (FEC) using the new molecular workflow for all herds and sampling periods. Finally, no effect of storage in 70% ethanol was detected on sedimentation and DNA isolation outcomes; enabling transport of samples from endemic to non-endemic countries without the requirement of a complete cold chain. The commercially-available ELISA displayed poorer sensitivity, even after adjustment of the positive threshold (65–88%), compared to the sensitivity (91–100%) of the new molecular diagnostic workflow. Conclusions/Significance Species-specific assays for sensitive detection of Fasciola spp. enable ante-mortem diagnosis in both human and animal settings. This includes Southeast Asia where there are potentially many undocumented human cases and where post-mortem examination of production animals can be difficult. The new molecular workflow provides a sensitive and quantitative diagnostic approach for the rapid testing of medium to large sample sizes, potentially superseding the traditional sedimentation and FEC technique and enabling surveillance programs in locations where animal and human health funding is limited. PMID:28915255

77 FR 28567 - Notice of Petitions by Firms for Determination of Eligibility To Apply for Trade Adjustment...

Federal Register 2010, 2011, 2012, 2013, 2014

2012-05-15

..., LLC......... Hubbardton Forge, 04/06/12 The firm designs and manufactures hand- LLC., P.O. Box 827... workers, or threat thereof, and to a decrease in sales or production of each petitioning firm. List of... U.S. Highway 90W, 04/23/12 The firm manufactures processed food for San Antonio, TX 78237. human...

78 FR 4879 - Nine Mile Point 3 Nuclear Project, LLC and UniStar Nuclear Operating Services, LLC Combined...

Federal Register 2010, 2011, 2012, 2013, 2014

2013-01-23

... Analysis Report (FSAR). On December 1, 2009, UniStar Nuclear Energy (UNE), acting on behalf of the COL... Exclusion From Environmental Review With respect to the exemption's impact on the quality of the human... relation to security issues. Therefore, the common defense and security is not impacted by this exemption...

Nexus: A modular workflow management system for quantum simulation codes

NASA Astrophysics Data System (ADS)

Krogel, Jaron T.

2016-01-01

The management of simulation workflows represents a significant task for the individual computational researcher. Automation of the required tasks involved in simulation work can decrease the overall time to solution and reduce sources of human error. A new simulation workflow management system, Nexus, is presented to address these issues. Nexus is capable of automated job management on workstations and resources at several major supercomputing centers. Its modular design allows many quantum simulation codes to be supported within the same framework. Current support includes quantum Monte Carlo calculations with QMCPACK, density functional theory calculations with Quantum Espresso or VASP, and quantum chemical calculations with GAMESS. Users can compose workflows through a transparent, text-based interface, resembling the input file of a typical simulation code. A usage example is provided to illustrate the process.

Support for Taverna workflows in the VPH-Share cloud platform.

PubMed

Kasztelnik, Marek; Coto, Ernesto; Bubak, Marian; Malawski, Maciej; Nowakowski, Piotr; Arenas, Juan; Saglimbeni, Alfredo; Testi, Debora; Frangi, Alejandro F

2017-07-01

To address the increasing need for collaborative endeavours within the Virtual Physiological Human (VPH) community, the VPH-Share collaborative cloud platform allows researchers to expose and share sequences of complex biomedical processing tasks in the form of computational workflows. The Taverna Workflow System is a very popular tool for orchestrating complex biomedical & bioinformatics processing tasks in the VPH community. This paper describes the VPH-Share components that support the building and execution of Taverna workflows, and explains how they interact with other VPH-Share components to improve the capabilities of the VPH-Share platform. Taverna workflow support is delivered by the Atmosphere cloud management platform and the VPH-Share Taverna plugin. These components are explained in detail, along with the two main procedures that were developed to enable this seamless integration: workflow composition and execution. 1) Seamless integration of VPH-Share with other components and systems. 2) Extended range of different tools for workflows. 3) Successful integration of scientific workflows from other VPH projects. 4) Execution speed improvement for medical applications. The presented workflow integration provides VPH-Share users with a wide range of different possibilities to compose and execute workflows, such as desktop or online composition, online batch execution, multithreading, remote execution, etc. The specific advantages of each supported tool are presented, as are the roles of Atmosphere and the VPH-Share plugin within the VPH-Share project. The combination of the VPH-Share plugin and Atmosphere engenders the VPH-Share infrastructure with far more flexible, powerful and usable capabilities for the VPH-Share community. As both components can continue to evolve and improve independently, we acknowledge that further improvements are still to be developed and will be described. Copyright © 2017 Elsevier B.V. All rights reserved.

recount workflow: Accessing over 70,000 human RNA-seq samples with Bioconductor

PubMed Central

Collado-Torres, Leonardo; Nellore, Abhinav; Jaffe, Andrew E.

2017-01-01

The recount2 resource is composed of over 70,000 uniformly processed human RNA-seq samples spanning TCGA and SRA, including GTEx. The processed data can be accessed via the recount2 website and the recountBioconductor package. This workflow explains in detail how to use the recountpackage and how to integrate it with other Bioconductor packages for several analyses that can be carried out with the recount2 resource. In particular, we describe how the coverage count matrices were computed in recount2 as well as different ways of obtaining public metadata, which can facilitate downstream analyses. Step-by-step directions show how to do a gene-level differential expression analysis, visualize base-level genome coverage data, and perform an analyses at multiple feature levels. This workflow thus provides further information to understand the data in recount2 and a compendium of R code to use the data. PMID:29043067

Modernizing Earth and Space Science Modeling Workflows in the Big Data Era

NASA Astrophysics Data System (ADS)

Kinter, J. L.; Feigelson, E.; Walker, R. J.; Tino, C.

2017-12-01

Modeling is a major aspect of the Earth and space science research. The development of numerical models of the Earth system, planetary systems or astrophysical systems is essential to linking theory with observations. Optimal use of observations that are quite expensive to obtain and maintain typically requires data assimilation that involves numerical models. In the Earth sciences, models of the physical climate system are typically used for data assimilation, climate projection, and inter-disciplinary research, spanning applications from analysis of multi-sensor data sets to decision-making in climate-sensitive sectors with applications to ecosystems, hazards, and various biogeochemical processes. In space physics, most models are from first principles, require considerable expertise to run and are frequently modified significantly for each case study. The volume and variety of model output data from modeling Earth and space systems are rapidly increasing and have reached a scale where human interaction with data is prohibitively inefficient. A major barrier to progress is that modeling workflows isn't deemed by practitioners to be a design problem. Existing workflows have been created by a slow accretion of software, typically based on undocumented, inflexible scripts haphazardly modified by a succession of scientists and students not trained in modern software engineering methods. As a result, existing modeling workflows suffer from an inability to onboard new datasets into models; an inability to keep pace with accelerating data production rates; and irreproducibility, among other problems. These factors are creating an untenable situation for those conducting and supporting Earth system and space science. Improving modeling workflows requires investments in hardware, software and human resources. This paper describes the critical path issues that must be targeted to accelerate modeling workflows, including script modularization, parallelization, and automation in the near term, and longer term investments in virtualized environments for improved scalability, tolerance for lossy data compression, novel data-centric memory and storage technologies, and tools for peer reviewing, preserving and sharing workflows, as well as fundamental statistical and machine learning algorithms.

REPRODUCIBLE RESEARCH WORKFLOW IN R FOR THE ANALYSIS OF PERSONALIZED HUMAN MICROBIOME DATA.

PubMed

Callahan, Benjamin; Proctor, Diana; Relman, David; Fukuyama, Julia; Holmes, Susan

2016-01-01

This article presents a reproducible research workflow for amplicon-based microbiome studies in personalized medicine created using Bioconductor packages and the knitr markdown interface.We show that sometimes a multiplicity of choices and lack of consistent documentation at each stage of the sequential processing pipeline used for the analysis of microbiome data can lead to spurious results. We propose its replacement with reproducible and documented analysis using R packages dada2, knitr, and phyloseq. This workflow implements both key stages of amplicon analysis: the initial filtering and denoising steps needed to construct taxonomic feature tables from error-containing sequencing reads (dada2), and the exploratory and inferential analysis of those feature tables and associated sample metadata (phyloseq). This workow facilitates reproducible interrogation of the full set of choices required in microbiome studies. We present several examples in which we leverage existing packages for analysis in a way that allows easy sharing and modification by others, and give pointers to articles that depend on this reproducible workflow for the study of longitudinal and spatial series analyses of the vaginal microbiome in pregnancy and the oral microbiome in humans with healthy dentition and intra-oral tissues.

Exploring Dental Providers’ Workflow in an Electronic Dental Record Environment

PubMed Central

Schwei, Kelsey M; Cooper, Ryan; Mahnke, Andrea N.; Ye, Zhan

2016-01-01

Summary Background A workflow is defined as a predefined set of work steps and partial ordering of these steps in any environment to achieve the expected outcome. Few studies have investigated the workflow of providers in a dental office. It is important to understand the interaction of dental providers with the existing technologies at point of care to assess breakdown in the workflow which could contribute to better technology designs. Objective The study objective was to assess electronic dental record (EDR) workflows using time and motion methodology in order to identify breakdowns and opportunities for process improvement. Methods A time and motion methodology was used to study the human-computer interaction and workflow of dental providers with an EDR in four dental centers at a large healthcare organization. A data collection tool was developed to capture the workflow of dental providers and staff while they interacted with an EDR during initial, planned, and emergency patient visits, and at the front desk. Qualitative and quantitative analysis was conducted on the observational data. Results Breakdowns in workflow were identified while posting charges, viewing radiographs, e-prescribing, and interacting with patient scheduler. EDR interaction time was significantly different between dentists and dental assistants (6:20 min vs. 10:57 min, p = 0.013) and between dentists and dental hygienists (6:20 min vs. 9:36 min, p = 0.003). Conclusions On average, a dentist spent far less time than dental assistants and dental hygienists in data recording within the EDR. PMID:27437058

Nexus: a modular workflow management system for quantum simulation codes

DOE PAGES

Krogel, Jaron T.

2015-08-24

The management of simulation workflows is a significant task for the individual computational researcher. Automation of the required tasks involved in simulation work can decrease the overall time to solution and reduce sources of human error. A new simulation workflow management system, Nexus, is presented to address these issues. Nexus is capable of automated job management on workstations and resources at several major supercomputing centers. Its modular design allows many quantum simulation codes to be supported within the same framework. Current support includes quantum Monte Carlo calculations with QMCPACK, density functional theory calculations with Quantum Espresso or VASP, and quantummore » chemical calculations with GAMESS. Users can compose workflows through a transparent, text-based interface, resembling the input file of a typical simulation code. A usage example is provided to illustrate the process.« less

Experimenting with semantic web services to understand the role of NLP technologies in healthcare.

PubMed

Jagannathan, V

2006-01-01

NLP technologies can play a significant role in healthcare where a predominant segment of the clinical documentation is in text form. In a graduate course focused on understanding semantic web services at West Virginia University, a class project was designed with the purpose of exploring potential use for NLP-based abstraction of clinical documentation. The role of NLP-technology was simulated using human abstractors and various workflows were investigated using public domain workflow and semantic web service technologies. This poster explores the potential use of NLP and the role of workflow and semantic web technologies in developing healthcare IT environments.

Human Systems Integration Design Environment (HSIDE)

DTIC Science & Technology

2012-04-09

quality of the resulting HSI products. 15. SUBJECT TERMS HSI , Manning Estimation and Validation , Risk Assessment, I POE, PLM, BPMN , Workflow...business process model in Business Process Modeling Notation ( BPMN ) or the actual workflow template associated with the specific functional area, again...as filtered by the user settings in the high level interface. Figure 3 shows the initial screen which allows the user to select either the BPMN or

Create, run, share, publish, and reference your LC-MS, FIA-MS, GC-MS, and NMR data analysis workflows with the Workflow4Metabolomics 3.0 Galaxy online infrastructure for metabolomics.

PubMed

Guitton, Yann; Tremblay-Franco, Marie; Le Corguillé, Gildas; Martin, Jean-François; Pétéra, Mélanie; Roger-Mele, Pierrick; Delabrière, Alexis; Goulitquer, Sophie; Monsoor, Misharl; Duperier, Christophe; Canlet, Cécile; Servien, Rémi; Tardivel, Patrick; Caron, Christophe; Giacomoni, Franck; Thévenot, Etienne A

2017-12-01

Metabolomics is a key approach in modern functional genomics and systems biology. Due to the complexity of metabolomics data, the variety of experimental designs, and the multiplicity of bioinformatics tools, providing experimenters with a simple and efficient resource to conduct comprehensive and rigorous analysis of their data is of utmost importance. In 2014, we launched the Workflow4Metabolomics (W4M; http://workflow4metabolomics.org) online infrastructure for metabolomics built on the Galaxy environment, which offers user-friendly features to build and run data analysis workflows including preprocessing, statistical analysis, and annotation steps. Here we present the new W4M 3.0 release, which contains twice as many tools as the first version, and provides two features which are, to our knowledge, unique among online resources. First, data from the four major metabolomics technologies (i.e., LC-MS, FIA-MS, GC-MS, and NMR) can be analyzed on a single platform. By using three studies in human physiology, alga evolution, and animal toxicology, we demonstrate how the 40 available tools can be easily combined to address biological issues. Second, the full analysis (including the workflow, the parameter values, the input data and output results) can be referenced with a permanent digital object identifier (DOI). Publication of data analyses is of major importance for robust and reproducible science. Furthermore, the publicly shared workflows are of high-value for e-learning and training. The Workflow4Metabolomics 3.0 e-infrastructure thus not only offers a unique online environment for analysis of data from the main metabolomics technologies, but it is also the first reference repository for metabolomics workflows. Copyright © 2017 Elsevier Ltd. All rights reserved.

United Space Alliance LLC Parachute Refurbishment Facility Model

NASA Technical Reports Server (NTRS)

Esser, Valerie; Pessaro, Martha; Young, Angela

2007-01-01

The Parachute Refurbishment Facility Model was created to reflect the flow of hardware through the facility using anticipated start and delivery times from a project level IV schedule. Distributions for task times were built using historical build data for SFOC work and new data generated for CLV/ARES task times. The model currently processes 633 line items from 14 SFOC builds for flight readiness, 16 SFOC builds returning from flight for defoul, wash, and dry operations, 12 builds for CLV manufacturing operations, and 1 ARES 1X build. Modeling the planned workflow through the PRF is providing a reliable way to predict the capability of the facility as well as the manpower resource need. Creating a real world process allows for real world problems to be identified and potential workarounds to be implemented in a safe, simulated world before taking it to the next step, implementation in the real world.

Structuring research methods and data with the research object model: genomics workflows as a case study.

PubMed

Hettne, Kristina M; Dharuri, Harish; Zhao, Jun; Wolstencroft, Katherine; Belhajjame, Khalid; Soiland-Reyes, Stian; Mina, Eleni; Thompson, Mark; Cruickshank, Don; Verdes-Montenegro, Lourdes; Garrido, Julian; de Roure, David; Corcho, Oscar; Klyne, Graham; van Schouwen, Reinout; 't Hoen, Peter A C; Bechhofer, Sean; Goble, Carole; Roos, Marco

2014-01-01

One of the main challenges for biomedical research lies in the computer-assisted integrative study of large and increasingly complex combinations of data in order to understand molecular mechanisms. The preservation of the materials and methods of such computational experiments with clear annotations is essential for understanding an experiment, and this is increasingly recognized in the bioinformatics community. Our assumption is that offering means of digital, structured aggregation and annotation of the objects of an experiment will provide necessary meta-data for a scientist to understand and recreate the results of an experiment. To support this we explored a model for the semantic description of a workflow-centric Research Object (RO), where an RO is defined as a resource that aggregates other resources, e.g., datasets, software, spreadsheets, text, etc. We applied this model to a case study where we analysed human metabolite variation by workflows. We present the application of the workflow-centric RO model for our bioinformatics case study. Three workflows were produced following recently defined Best Practices for workflow design. By modelling the experiment as an RO, we were able to automatically query the experiment and answer questions such as "which particular data was input to a particular workflow to test a particular hypothesis?", and "which particular conclusions were drawn from a particular workflow?". Applying a workflow-centric RO model to aggregate and annotate the resources used in a bioinformatics experiment, allowed us to retrieve the conclusions of the experiment in the context of the driving hypothesis, the executed workflows and their input data. The RO model is an extendable reference model that can be used by other systems as well. The Research Object is available at http://www.myexperiment.org/packs/428 The Wf4Ever Research Object Model is available at http://wf4ever.github.io/ro.

Protein kinetic signatures of the remodeling heart following isoproterenol stimulation.

PubMed

Lam, Maggie P Y; Wang, Ding; Lau, Edward; Liem, David A; Kim, Allen K; Ng, Dominic C M; Liang, Xiangbo; Bleakley, Brian J; Liu, Chenguang; Tabaraki, Jason D; Cadeiras, Martin; Wang, Yibin; Deng, Mario C; Ping, Peipei

2014-04-01

Protein temporal dynamics play a critical role in time-dimensional pathophysiological processes, including the gradual cardiac remodeling that occurs in early-stage heart failure. Methods for quantitative assessments of protein kinetics are lacking, and despite knowledge gained from single-protein studies, integrative views of the coordinated behavior of multiple proteins in cardiac remodeling are scarce. Here, we developed a workflow that integrates deuterium oxide (2H2O) labeling, high-resolution mass spectrometry (MS), and custom computational methods to systematically interrogate in vivo protein turnover. Using this workflow, we characterized the in vivo turnover kinetics of 2,964 proteins in a mouse model of β-adrenergic-induced cardiac remodeling. The data provided a quantitative and longitudinal view of cardiac remodeling at the molecular level, revealing widespread kinetic regulations in calcium signaling, metabolism, proteostasis, and mitochondrial dynamics. We translated the workflow to human studies, creating a reference dataset of 496 plasma protein turnover rates from 4 healthy adults. The approach is applicable to short, minimal label enrichment and can be performed on as little as a single biopsy, thereby overcoming critical obstacles to clinical investigations. The protein turnover quantitation experiments and computational workflow described here should be widely applicable to large-scale biomolecular investigations of human disease mechanisms with a temporal perspective.

Protein kinetic signatures of the remodeling heart following isoproterenol stimulation

PubMed Central

Lam, Maggie P.Y.; Wang, Ding; Lau, Edward; Liem, David A.; Kim, Allen K.; Ng, Dominic C.M.; Liang, Xiangbo; Bleakley, Brian J.; Liu, Chenguang; Tabaraki, Jason D.; Cadeiras, Martin; Wang, Yibin; Deng, Mario C.; Ping, Peipei

2014-01-01

Protein temporal dynamics play a critical role in time-dimensional pathophysiological processes, including the gradual cardiac remodeling that occurs in early-stage heart failure. Methods for quantitative assessments of protein kinetics are lacking, and despite knowledge gained from single-protein studies, integrative views of the coordinated behavior of multiple proteins in cardiac remodeling are scarce. Here, we developed a workflow that integrates deuterium oxide (2H2O) labeling, high-resolution mass spectrometry (MS), and custom computational methods to systematically interrogate in vivo protein turnover. Using this workflow, we characterized the in vivo turnover kinetics of 2,964 proteins in a mouse model of β-adrenergic–induced cardiac remodeling. The data provided a quantitative and longitudinal view of cardiac remodeling at the molecular level, revealing widespread kinetic regulations in calcium signaling, metabolism, proteostasis, and mitochondrial dynamics. We translated the workflow to human studies, creating a reference dataset of 496 plasma protein turnover rates from 4 healthy adults. The approach is applicable to short, minimal label enrichment and can be performed on as little as a single biopsy, thereby overcoming critical obstacles to clinical investigations. The protein turnover quantitation experiments and computational workflow described here should be widely applicable to large-scale biomolecular investigations of human disease mechanisms with a temporal perspective. PMID:24614109

Redesign of a computerized clinical reminder for colorectal cancer screening: a human-computer interaction evaluation

PubMed Central

2011-01-01

Background Based on barriers to the use of computerized clinical decision support (CDS) learned in an earlier field study, we prototyped design enhancements to the Veterans Health Administration's (VHA's) colorectal cancer (CRC) screening clinical reminder to compare against the VHA's current CRC reminder. Methods In a controlled simulation experiment, 12 primary care providers (PCPs) used prototypes of the current and redesigned CRC screening reminder in a within-subject comparison. Quantitative measurements were based on a usability survey, workload assessment instrument, and workflow integration survey. We also collected qualitative data on both designs. Results Design enhancements to the VHA's existing CRC screening clinical reminder positively impacted aspects of usability and workflow integration but not workload. The qualitative analysis revealed broad support across participants for the design enhancements with specific suggestions for improving the reminder further. Conclusions This study demonstrates the value of a human-computer interaction evaluation in informing the redesign of information tools to foster uptake, integration into workflow, and use in clinical practice. PMID:22126324

Comprehensive and quantitative profiling of lipid species in human milk, cow milk and a phospholipid-enriched milk formula by GC and MS/MSALL.

PubMed

Sokol, Elena; Ulven, Trond; Færgeman, Nils J; Ejsing, Christer S

2015-06-01

Here we present a workflow for in-depth analysis of milk lipids that combines gas chromatography (GC) for fatty acid (FA) profiling and a shotgun lipidomics routine termed MS/MS ALL for structural characterization of molecular lipid species. To evaluate the performance of the workflow we performed a comparative lipid analysis of human milk, cow milk, and Lacprodan® PL-20, a phospholipid-enriched milk protein concentrate for infant formula. The GC analysis showed that human milk and Lacprodan have a similar FA profile with higher levels of unsaturated FAs as compared to cow milk. In-depth lipidomic analysis by MS/MS ALL revealed that each type of milk sample comprised distinct composition of molecular lipid species. Lipid class composition showed that the human and cow milk contain a higher proportion of triacylglycerols (TAGs) as compared to Lacprodan. Notably, the MS/MS ALL analysis demonstrated that the similar FA profile of human milk and Lacprodan determined by GC analysis is attributed to the composition of individual TAG species in human milk and glycerophospholipid species in Lacprodan. Moreover, the analysis of TAG molecules in Lacprodan and cow milk showed a high proportion of short-chain FAs that could not be monitored by GC analysis. The results presented here show that complementary GC and MS/MS ALL analysis is a powerful approach for characterization of molecular lipid species in milk and milk products. : Milk lipid analysis is routinely performed using gas chromatography. This method reports the total fatty acid composition of all milk lipids, but provides no structural or quantitative information about individual lipid molecules in milk or milk products. Here we present a workflow that integrates gas chromatography for fatty acid profiling and a shotgun lipidomics routine termed MS/MS ALL for structural analysis and quantification of molecular lipid species. We demonstrate the efficacy of this complementary workflow by a comparative analysis of molecular lipid species in human milk, cow milk, and a milk-based supplement used for infant formula.

Comprehensive and quantitative profiling of lipid species in human milk, cow milk and a phospholipid-enriched milk formula by GC and MS/MSALL

PubMed Central

Sokol, Elena; Ulven, Trond; Færgeman, Nils J; Ejsing, Christer S

2015-01-01

Here we present a workflow for in-depth analysis of milk lipids that combines gas chromatography (GC) for fatty acid (FA) profiling and a shotgun lipidomics routine termed MS/MSALL for structural characterization of molecular lipid species. To evaluate the performance of the workflow we performed a comparative lipid analysis of human milk, cow milk, and Lacprodan® PL-20, a phospholipid-enriched milk protein concentrate for infant formula. The GC analysis showed that human milk and Lacprodan have a similar FA profile with higher levels of unsaturated FAs as compared to cow milk. In-depth lipidomic analysis by MS/MSALL revealed that each type of milk sample comprised distinct composition of molecular lipid species. Lipid class composition showed that the human and cow milk contain a higher proportion of triacylglycerols (TAGs) as compared to Lacprodan. Notably, the MS/MSALL analysis demonstrated that the similar FA profile of human milk and Lacprodan determined by GC analysis is attributed to the composition of individual TAG species in human milk and glycerophospholipid species in Lacprodan. Moreover, the analysis of TAG molecules in Lacprodan and cow milk showed a high proportion of short-chain FAs that could not be monitored by GC analysis. The results presented here show that complementary GC and MS/MSALL analysis is a powerful approach for characterization of molecular lipid species in milk and milk products. Practical applications : Milk lipid analysis is routinely performed using gas chromatography. This method reports the total fatty acid composition of all milk lipids, but provides no structural or quantitative information about individual lipid molecules in milk or milk products. Here we present a workflow that integrates gas chromatography for fatty acid profiling and a shotgun lipidomics routine termed MS/MSALL for structural analysis and quantification of molecular lipid species. We demonstrate the efficacy of this complementary workflow by a comparative analysis of molecular lipid species in human milk, cow milk, and a milk-based supplement used for infant formula. PMID:26089741

Differences in the expression of endogenous efflux transporters in MDR1-transfected versus wildtype cell lines affect P-glycoprotein mediated drug transport

PubMed Central

Kuteykin-Teplyakov, Konstantin; Luna-Tortós, Carlos; Ambroziak, Kamila; Löscher, Wolfgang

2010-01-01

Background and purpose: P-glycoprotein (Pgp) efflux assays are widely used to identify Pgp substrates. The kidney cell lines Madin-Darby canine kidney (MDCK)-II and LLC-PK1, transfected with human MDR1 (ABCB1) are used to provide recombinant models of drug transport. Endogenous transporters in these cells may contribute to the activities of recombinant transporters, so that drug transport in MDR1-transfected cells is often corrected for the transport obtained in parental (wildtype) cells. However, expression of endogenous transporters may vary between transfected and wildtype cells, so that this correction may cause erroneous data. Here, we have measured the expression of endogenous efflux transporters in transfected and wildtype MDCK-II or LLC cells and the consequences for Pgp-mediated drug transport. Experimental approach: Using quantitative real-time RT-PCR, we determined the expression of endogenous Mdr1 mRNA and other efflux transporters in wildtype and MDR1-transfected MDCK-II and LLC cells. Transcellular transport was measured with the test substrate vinblastine. Key results: In MDR1-transfected MDCK cells, expression of endogenous (canine) Mdr1 and Mrp2 (Abcc2) mRNA was markedly lower than in wildtype cells, whereas MDR1-transfected LLC cells exhibited comparable Mdr1 but strikingly higher Mrp2 mRNA levels than wildtype cells. As a consequence, transport of vinblastine by human Pgp in efflux experiments was markedly underestimated when transport in MDR1-transfected MDCK cells was corrected for transport obtained in wildtype cells. This problem did not occur in LLC cells. Conclusions and implications: Differences in the expression of endogenous efflux transporters between transfected and wildtype MDCK cells provide a potential bias for in vitro studies on Pgp-mediated drug transport. PMID:20590635

Blonanserin, a novel atypical antipsychotic agent not actively transported as substrate by P-glycoprotein.

PubMed

Inoue, Tomoko; Osada, Kenichi; Tagawa, Masaaki; Ogawa, Yuriko; Haga, Toshiaki; Sogame, Yoshihisa; Hashizume, Takanori; Watanabe, Takashi; Taguchi, Atsushi; Katsumata, Takashi; Yabuki, Masashi; Yamaguchi, Noboru

2012-10-01

Although blonanserin, a novel atypical antipsychotic agent with dopamine D(2)/serotonin 5-HT(2A) antagonistic properties, displays good brain distribution, the mechanism of this distribution has not been clarified. P-glycoprotein [(P-gp) or multidrug resistance protein 1 (MDR1)] is an efflux transporter expressed in the brain and plays an important role in limiting drug entry into the central nervous system (CNS). In particular, P-gp can affect the pharmacokinetics and efficacy of antipsychotics, and exacerbate or soothe their adverse effects. In this study, we conducted in vitro and in vivo experiments to determine whether blonanserin is a P-gp substrate. Risperidone and its active metabolite 9-hydroxyrisperidone, both of which are P-gp substrates, were used as reference drugs. Affinity of blonanserin, risperidone, and 9-hydroxyrisperidone for P-gp was evaluated by in vitro transcellular transport across LLC-PK1, human MDR1 cDNA-transfected LLC-PK1 (LLC-MDR1), and mouse Mdr1a cDNA-transfected LLC-PK1 (LLC-Mdr1a). In addition, pharmacokinetic parameters in the brain and plasma (B/P ratio) of test compounds were measured in mdr1a/1b knockout (KO) and wild-type (WT) mice. The results of in vitro experiments revealed that P-gp does not actively transport blonanserin as a substrate in humans or mice. In addition, blonanserin displayed comparable B/P ratios in KO and WT mice, whereas B/P ratios of risperidone and 9-hydroxyrisperidone differed markedly in these animals. Our results indicate that blonanserin is not a P-gp substrate and therefore its brain distribution is unlikely to be affected by this transporter. Copyright © 2012 Elsevier Inc. All rights reserved.

Automation of lidar-based hydrologic feature extraction workflows using GIS

NASA Astrophysics Data System (ADS)

Borlongan, Noel Jerome B.; de la Cruz, Roel M.; Olfindo, Nestor T.; Perez, Anjillyn Mae C.

2016-10-01

With the advent of LiDAR technology, higher resolution datasets become available for use in different remote sensing and GIS applications. One significant application of LiDAR datasets in the Philippines is in resource features extraction. Feature extraction using LiDAR datasets require complex and repetitive workflows which can take a lot of time for researchers through manual execution and supervision. The Development of the Philippine Hydrologic Dataset for Watersheds from LiDAR Surveys (PHD), a project under the Nationwide Detailed Resources Assessment Using LiDAR (Phil-LiDAR 2) program, created a set of scripts, the PHD Toolkit, to automate its processes and workflows necessary for hydrologic features extraction specifically Streams and Drainages, Irrigation Network, and Inland Wetlands, using LiDAR Datasets. These scripts are created in Python and can be added in the ArcGIS® environment as a toolbox. The toolkit is currently being used as an aid for the researchers in hydrologic feature extraction by simplifying the workflows, eliminating human errors when providing the inputs, and providing quick and easy-to-use tools for repetitive tasks. This paper discusses the actual implementation of different workflows developed by Phil-LiDAR 2 Project 4 in Streams, Irrigation Network and Inland Wetlands extraction.

Towards an intelligent hospital environment: OR of the future.

PubMed

Sutherland, Jeffrey V; van den Heuvel, Willem-Jan; Ganous, Tim; Burton, Matthew M; Kumar, Animesh

2005-01-01

Patients, providers, payers, and government demand more effective and efficient healthcare services, and the healthcare industry needs innovative ways to re-invent core processes. Business process reengineering (BPR) showed adopting new hospital information systems can leverage this transformation and workflow management technologies can automate process management. Our research indicates workflow technologies in healthcare require real time patient monitoring, detection of adverse events, and adaptive responses to breakdown in normal processes. Adaptive workflow systems are rarely implemented making current workflow implementations inappropriate for healthcare. The advent of evidence based medicine, guideline based practice, and better understanding of cognitive workflow combined with novel technologies including Radio Frequency Identification (RFID), mobile/wireless technologies, internet workflow, intelligent agents, and Service Oriented Architectures (SOA) opens up new and exciting ways of automating business processes. Total situational awareness of events, timing, and location of healthcare activities can generate self-organizing change in behaviors of humans and machines. A test bed of a novel approach towards continuous process management was designed for the new Weinburg Surgery Building at the University of Maryland Medical. Early results based on clinical process mapping and analysis of patient flow bottlenecks demonstrated 100% improvement in delivery of supplies and instruments at surgery start time. This work has been directly applied to the design of the DARPA Trauma Pod research program where robotic surgery will be performed on wounded soldiers on the battlefield.

DISPELLING MYTHS AND MISCONCEPTIONS TO IMPLEMENT A SAFETY CULTURE

DOE Office of Scientific and Technical Information (OSTI.GOV)

Potts, T. Todd; Smith, Ken; Hylko, James M.

2003-02-27

Industrial accidents are typically reported in terms of technological malfunctions, ignoring the human element in accident causation. However, over two-thirds of all accidents are attributable to human and organizational factors (e.g., planning, written procedures, job factors, training, communication, and teamwork), thereby affecting risk perception, behavior and attitudes. This paper reviews the development of WESKEM, LLC's Environmental, Safety, and Health (ES&H) Program that addresses human and organizational factors from a top-down, bottom-up approach. This approach is derived from the Department of Energy's Integrated Safety Management System. As a result, dispelling common myths and misconceptions about safety, while empowering employees to ''STOPmore » work'' if necessary, have contributed to reducing an unusually high number of vehicle, ergonomic and slip/trip/fall incidents successfully. Furthermore, the safety culture that has developed within WESKEM, LLC's workforce consists of three common characteristics: (1) all employees hold safety as a value; (2) each individual feels responsible for the safety of their co-workers as well as themselves; and (3) each individual is willing and able to ''go beyond the call of duty'' on behalf of the safety of others. WESKEM, LLC as a company, upholds the safety culture and continues to enhance its existing ES&H program by incorporating employee feedback and lessons learned collected from other high-stress industries, thereby protecting its most vital resource - the employees. The success of this program is evident by reduced accident and injury rates, as well as the number of safe work hours accrued while performing hands-on field activities. WESKEM, LLC (Paducah + Oak Ridge) achieved over 800,000 safe work hours through August 2002. WESKEM-Paducah has achieved over 665,000 safe work hours without a recordable injury or lost workday case since it started operations on February 28, 2000.« less

The medical simulation markup language - simplifying the biomechanical modeling workflow.

PubMed

Suwelack, Stefan; Stoll, Markus; Schalck, Sebastian; Schoch, Nicolai; Dillmann, Rüdiger; Bendl, Rolf; Heuveline, Vincent; Speidel, Stefanie

2014-01-01

Modeling and simulation of the human body by means of continuum mechanics has become an important tool in diagnostics, computer-assisted interventions and training. This modeling approach seeks to construct patient-specific biomechanical models from tomographic data. Usually many different tools such as segmentation and meshing algorithms are involved in this workflow. In this paper we present a generalized and flexible description for biomechanical models. The unique feature of the new modeling language is that it not only describes the final biomechanical simulation, but also the workflow how the biomechanical model is constructed from tomographic data. In this way, the MSML can act as a middleware between all tools used in the modeling pipeline. The MSML thus greatly facilitates the prototyping of medical simulation workflows for clinical and research purposes. In this paper, we not only detail the XML-based modeling scheme, but also present a concrete implementation. Different examples highlight the flexibility, robustness and ease-of-use of the approach.

A Community-Driven Workflow Recommendations and Reuse Infrastructure

NASA Astrophysics Data System (ADS)

Zhang, J.; Votava, P.; Lee, T. J.; Lee, C.; Xiao, S.; Nemani, R. R.; Foster, I.

2013-12-01

Aiming to connect the Earth science community to accelerate the rate of discovery, NASA Earth Exchange (NEX) has established an online repository and platform, so that researchers can publish and share their tools and models with colleagues. In recent years, workflow has become a popular technique at NEX for Earth scientists to define executable multi-step procedures for data processing and analysis. The ability to discover and reuse knowledge (sharable workflows or workflow) is critical to the future advancement of science. However, as reported in our earlier study, the reusability of scientific artifacts at current time is very low. Scientists often do not feel confident in using other researchers' tools and utilities. One major reason is that researchers are often unaware of the existence of others' data preprocessing processes. Meanwhile, researchers often do not have time to fully document the processes and expose them to others in a standard way. These issues cannot be overcome by the existing workflow search technologies used in NEX and other data projects. Therefore, this project aims to develop a proactive recommendation technology based on collective NEX user behaviors. In this way, we aim to promote and encourage process and workflow reuse within NEX. Particularly, we focus on leveraging peer scientists' best practices to support the recommendation of artifacts developed by others. Our underlying theoretical foundation is rooted in the social cognitive theory, which declares people learn by watching what others do. Our fundamental hypothesis is that sharable artifacts have network properties, much like humans in social networks. More generally, reusable artifacts form various types of social relationships (ties), and may be viewed as forming what organizational sociologists who use network analysis to study human interactions call a 'knowledge network.' In particular, we will tackle two research questions: R1: What hidden knowledge may be extracted from usage history to help Earth scientists better understand existing artifacts and how to use them in a proper manner? R2: Informed by insights derived from their computing contexts, how could such hidden knowledge be used to facilitate artifact reuse by Earth scientists? Our study of the two research questions will provide answers to three technical questions aiming to assist NEX users during workflow development: 1) How to determine what topics interest the researcher? 2) How to find appropriate artifacts? and 3) How to advise the researcher in artifact reuse? In this paper, we report our on-going efforts of leveraging social networking theory and analysis techniques to provide dynamic advice on artifact reuse to NEX users based on their surrounding contexts. As a proof of concept, we have designed and developed a plug-in to the VisTrails workflow design tool. When users develop workflows using VisTrails, our plug-in will proactively recommend most relevant sub-workflows to the users.

Process Mining for Individualized Behavior Modeling Using Wireless Tracking in Nursing Homes

PubMed Central

Fernández-Llatas, Carlos; Benedi, José-Miguel; García-Gómez, Juan M.; Traver, Vicente

2013-01-01

The analysis of human behavior patterns is increasingly used for several research fields. The individualized modeling of behavior using classical techniques requires too much time and resources to be effective. A possible solution would be the use of pattern recognition techniques to automatically infer models to allow experts to understand individual behavior. However, traditional pattern recognition algorithms infer models that are not readily understood by human experts. This limits the capacity to benefit from the inferred models. Process mining technologies can infer models as workflows, specifically designed to be understood by experts, enabling them to detect specific behavior patterns in users. In this paper, the eMotiva process mining algorithms are presented. These algorithms filter, infer and visualize workflows. The workflows are inferred from the samples produced by an indoor location system that stores the location of a resident in a nursing home. The visualization tool is able to compare and highlight behavior patterns in order to facilitate expert understanding of human behavior. This tool was tested with nine real users that were monitored for a 25-week period. The results achieved suggest that the behavior of users is continuously evolving and changing and that this change can be measured, allowing for behavioral change detection. PMID:24225907

Preservation of protein fluorescence in embedded human dendritic cells for targeted 3D light and electron microscopy

PubMed Central

HÖHN, K.; FUCHS, J.; FRÖBER, A.; KIRMSE, R.; GLASS, B.; ANDERS‐ÖSSWEIN, M.; WALTHER, P.; KRÄUSSLICH, H.‐G.

2015-01-01

Summary In this study, we present a correlative microscopy workflow to combine detailed 3D fluorescence light microscopy data with ultrastructural information gained by 3D focused ion beam assisted scanning electron microscopy. The workflow is based on an optimized high pressure freezing/freeze substitution protocol that preserves good ultrastructural detail along with retaining the fluorescence signal in the resin embedded specimens. Consequently, cellular structures of interest can readily be identified and imaged by state of the art 3D confocal fluorescence microscopy and are precisely referenced with respect to an imprinted coordinate system on the surface of the resin block. This allows precise guidance of the focused ion beam assisted scanning electron microscopy and limits the volume to be imaged to the structure of interest. This, in turn, minimizes the total acquisition time necessary to conduct the time consuming ultrastructural scanning electron microscope imaging while eliminating the risk to miss parts of the target structure. We illustrate the value of this workflow for targeting virus compartments, which are formed in HIV‐pulsed mature human dendritic cells. PMID:25786567

LipidFinder: A computational workflow for discovery of lipids identifies eicosanoid-phosphoinositides in platelets

PubMed Central

O’Connor, Anne; Brasher, Christopher J.; Slatter, David A.; Meckelmann, Sven W.; Hawksworth, Jade I.; Allen, Stuart M.; O’Donnell, Valerie B.

2017-01-01

Accurate and high-quality curation of lipidomic datasets generated from plasma, cells, or tissues is becoming essential for cell biology investigations and biomarker discovery for personalized medicine. However, a major challenge lies in removing artifacts otherwise mistakenly interpreted as real lipids from large mass spectrometry files (>60 K features), while retaining genuine ions in the dataset. This requires powerful informatics tools; however, available workflows have not been tailored specifically for lipidomics, particularly discovery research. We designed LipidFinder, an open-source Python workflow. An algorithm is included that optimizes analysis based on users’ own data, and outputs are screened against online databases and categorized into LIPID MAPS classes. LipidFinder outperformed three widely used metabolomics packages using data from human platelets. We show a family of three 12-hydroxyeicosatetraenoic acid phosphoinositides (16:0/, 18:1/, 18:0/12-HETE-PI) generated by thrombin-activated platelets, indicating crosstalk between eicosanoid and phosphoinositide pathways in human cells. The software is available on GitHub (https://github.com/cjbrasher/LipidFinder), with full userguides. PMID:28405621

An MRM-based workflow for quantifying cardiac mitochondrial protein phosphorylation in murine and human tissue.

PubMed

Lam, Maggie P Y; Scruggs, Sarah B; Kim, Tae-Young; Zong, Chenggong; Lau, Edward; Wang, Ding; Ryan, Christopher M; Faull, Kym F; Ping, Peipei

2012-08-03

The regulation of mitochondrial function is essential for cardiomyocyte adaptation to cellular stress. While it has long been understood that phosphorylation regulates flux through metabolic pathways, novel phosphorylation sites are continually being discovered in all functionally distinct areas of the mitochondrial proteome. Extracting biologically meaningful information from these phosphorylation sites requires an adaptable, sensitive, specific and robust method for their quantification. Here we report a multiple reaction monitoring-based mass spectrometric workflow for quantifying site-specific phosphorylation of mitochondrial proteins. Specifically, chromatographic and mass spectrometric conditions for 68 transitions derived from 23 murine and human phosphopeptides, and their corresponding unmodified peptides, were optimized. These methods enabled the quantification of endogenous phosphopeptides from the outer mitochondrial membrane protein VDAC, and the inner membrane proteins ANT and ETC complexes I, III and V. The development of this quantitative workflow is a pivotal step for advancing our knowledge and understanding of the regulatory effects of mitochondrial protein phosphorylation in cardiac physiology and pathophysiology. This article is part of a Special Issue entitled: Translational Proteomics. Copyright © 2012 Elsevier B.V. All rights reserved.

Software for rapid prototyping in the pharmaceutical and biotechnology industries.

PubMed

Kappler, Michael A

2008-05-01

The automation of drug discovery methods continues to develop, especially techniques that process information, represent workflow and facilitate decision-making. The magnitude of data and the plethora of questions in pharmaceutical and biotechnology research give rise to the need for rapid prototyping software. This review describes the advantages and disadvantages of three solutions: Competitive Workflow, Taverna and Pipeline Pilot. Each of these systems processes large amounts of data, integrates diverse systems and assists novice programmers and human experts in critical decision-making steps.

Modeling workflow to design machine translation applications for public health practice

PubMed Central

Turner, Anne M.; Brownstein, Megumu K.; Cole, Kate; Karasz, Hilary; Kirchhoff, Katrin

2014-01-01

Objective Provide a detailed understanding of the information workflow processes related to translating health promotion materials for limited English proficiency individuals in order to inform the design of context-driven machine translation (MT) tools for public health (PH). Materials and Methods We applied a cognitive work analysis framework to investigate the translation information workflow processes of two large health departments in Washington State. Researchers conducted interviews, performed a task analysis, and validated results with PH professionals to model translation workflow and identify functional requirements for a translation system for PH. Results The study resulted in a detailed description of work related to translation of PH materials, an information workflow diagram, and a description of attitudes towards MT technology. We identified a number of themes that hold design implications for incorporating MT in PH translation practice. A PH translation tool prototype was designed based on these findings. Discussion This study underscores the importance of understanding the work context and information workflow for which systems will be designed. Based on themes and translation information workflow processes, we identified key design guidelines for incorporating MT into PH translation work. Primary amongst these is that MT should be followed by human review for translations to be of high quality and for the technology to be adopted into practice. Counclusion The time and costs of creating multilingual health promotion materials are barriers to translation. PH personnel were interested in MT's potential to improve access to low-cost translated PH materials, but expressed concerns about ensuring quality. We outline design considerations and a potential machine translation tool to best fit MT systems into PH practice. PMID:25445922

77 FR 50095 - Combined Notice of Filings #2

Federal Register 2010, 2011, 2012, 2013, 2014

2012-08-20

... Combine Hills I LLC, Avenal Park LLC, Sand Drag LLC, Sun City Project LLC, Eurus Combine Hills II LLC... Power Partners, LLC, Crescent Ridge LLC, Eurus Combine Hills I LLC, Avenal Park LLC, Sand Drag LLC, Sun..., Eurus Combine Hills I LLC, Avenal Park LLC, Sand Drag LLC, Sun City Project LLC, Eurus Combine Hills II...

A Workflow to Investigate Exposure and Pharmacokinetic ...

EPA Pesticide Factsheets

Background: Adverse outcome pathways (AOPs) link adverse effects in individuals or populations to a molecular initiating event (MIE) that can be quantified using in vitro methods. Practical application of AOPs in chemical-specific risk assessment requires incorporation of knowledge on exposure, along with absorption, distribution, metabolism, and excretion (ADME) properties of chemicals.Objectives: We developed a conceptual workflow to examine exposure and ADME properties in relation to an MIE. The utility of this workflow was evaluated using a previously established AOP, acetylcholinesterase (AChE) inhibition.Methods: Thirty chemicals found to inhibit human AChE in the ToxCast™ assay were examined with respect to their exposure, absorption potential, and ability to cross the blood–brain barrier (BBB). Structures of active chemicals were compared against structures of 1,029 inactive chemicals to detect possible parent compounds that might have active metabolites.Results: Application of the workflow screened 10 “low-priority” chemicals of 30 active chemicals. Fifty-two of the 1,029 inactive chemicals exhibited a similarity threshold of ≥ 75% with their nearest active neighbors. Of these 52 compounds, 30 were excluded due to poor absorption or distribution. The remaining 22 compounds may inhibit AChE in vivo either directly or as a result of metabolic activation.Conclusions: The incorporation of exposure and ADME properties into the conceptual workflow e

77 FR 29633 - Alta Wind VII, LLC, Alta Wind IX, LLC, Alta Wind X, LLC, Alta Wind XI, LLC, Alta Wind XII, LLC...

Federal Register 2010, 2011, 2012, 2013, 2014

2012-05-18

... DEPARTMENT OF ENERGY Federal Energy Regulatory Commission [Docket No. EL12-68-000] Alta Wind VII, LLC, Alta Wind IX, LLC, Alta Wind X, LLC, Alta Wind XI, LLC, Alta Wind XII, LLC, Alta Wind XIII, LLC, Alta Wind XIV, LLC, Alta Wind XV, LLC, Alta Windpower Development, LLC, TGP Development Company, LLC...

75 FR 23263 - Alta Wind I, LLC; Alta Wind II, LLC; Alta Wind III, LLC; Alta Wind IV, LLC; Alta Wind V, LLC...

Federal Register 2010, 2011, 2012, 2013, 2014

2010-05-03

... DEPARTMENT OF ENERGY Federal Energy Regulatory Commission [Docket No. EL10-62-000] Alta Wind I, LLC; Alta Wind II, LLC; Alta Wind III, LLC; Alta Wind IV, LLC; Alta Wind V, LLC; Alta Wind VI, LLC; Alta Wind VII, LLC; Alta Wind VIII, LLC; Alta Windpower Development, LLC; TGP Development Company, LLC...

An integrated workflow to assess technical and biological variability of cell population frequencies in human peripheral blood by flow cytometry

PubMed Central

Burel, Julie G.; Qian, Yu; Arlehamn, Cecilia Lindestam; Weiskopf, Daniela; Zapardiel-Gonzalo, Jose; Taplitz, Randy; Gilman, Robert H.; Saito, Mayuko; de Silva, Aruna D.; Vijayanand, Pandurangan; Scheuermann, Richard H.; Sette, Alessandro; Peters, Bjoern

2016-01-01

In the context of large-scale human system immunology studies, controlling for technical and biological variability is crucial to ensure that experimental data support research conclusions. Here, we report on a universal workflow to evaluate both technical and biological variation in multiparameter flow cytometry, applied to the development of a 10-color panel to identify all major cell populations and T cell subsets in cryopreserved PBMC. Replicate runs from a control donation and comparison of different gating strategies assessed technical variability associated with each cell population and permitted the calculation of a quality control score. Applying our panel to a large collection of PBMC samples, we found that most cell populations showed low intra-individual variability over time. In contrast, certain subpopulations such as CD56 T cells and Temra CD4 T cells were associated with high inter-individual variability. Age but not gender had a significant effect on the frequency of several populations, with a drastic decrease in naïve T cells observed in older donors. Ethnicity also influenced a significant proportion of immune cell population frequencies, emphasizing the need to account for these co-variates in immune profiling studies. Finally, we exemplify the usefulness of our workflow by identifying a novel cell-subset signature of latent tuberculosis infection. Thus, our study provides a universal workflow to establish and evaluate any flow cytometry panel in systems immunology studies. PMID:28069807

An Integrated Workflow To Assess Technical and Biological Variability of Cell Population Frequencies in Human Peripheral Blood by Flow Cytometry.

PubMed

Burel, Julie G; Qian, Yu; Lindestam Arlehamn, Cecilia; Weiskopf, Daniela; Zapardiel-Gonzalo, Jose; Taplitz, Randy; Gilman, Robert H; Saito, Mayuko; de Silva, Aruna D; Vijayanand, Pandurangan; Scheuermann, Richard H; Sette, Alessandro; Peters, Bjoern

2017-02-15

In the context of large-scale human system immunology studies, controlling for technical and biological variability is crucial to ensure that experimental data support research conclusions. In this study, we report on a universal workflow to evaluate both technical and biological variation in multiparameter flow cytometry, applied to the development of a 10-color panel to identify all major cell populations and T cell subsets in cryopreserved PBMC. Replicate runs from a control donation and comparison of different gating strategies assessed the technical variability associated with each cell population and permitted the calculation of a quality control score. Applying our panel to a large collection of PBMC samples, we found that most cell populations showed low intraindividual variability over time. In contrast, certain subpopulations such as CD56 T cells and Temra CD4 T cells were associated with high interindividual variability. Age but not gender had a significant effect on the frequency of several populations, with a drastic decrease in naive T cells observed in older donors. Ethnicity also influenced a significant proportion of immune cell population frequencies, emphasizing the need to account for these covariates in immune profiling studies. We also exemplify the usefulness of our workflow by identifying a novel cell-subset signature of latent tuberculosis infection. Thus, our study provides a universal workflow to establish and evaluate any flow cytometry panel in systems immunology studies. Copyright © 2017 by The American Association of Immunologists, Inc.

The biodigital human: a web-based 3D platform for medical visualization and education.

PubMed

Qualter, John; Sculli, Frank; Oliker, Aaron; Napier, Zachary; Lee, Sabrina; Garcia, Julio; Frenkel, Sally; Harnik, Victoria; Triola, Marc

2012-01-01

NYU School of Medicine's Division of Educational Informatics in collaboration with BioDigital Systems LLC (New York, NY) has created a virtual human body dataset that is being used for visualization, education and training and is accessible over modern web browsers.

75 FR 9199 - Combined Notice of Filings #1

Federal Register 2010, 2011, 2012, 2013, 2014

2010-03-01

..., LLC, Calpine Power America--CA, LLC, Calpine Power America--OR, LLC,CES Marketing IX, LLC,CES Marketing V, L.P.,CES Marketing X, LLC, PCF2, LLC, Mankato Energy Center, LLC, Riverside Energy Center, LLC... Center, LLC, Pine Bluff Energy, LLC, Pastoria Energy Center, LLC, Morgan Energy Center, LLC, MOBILE...

77 FR 40608 - Notice of Petition for Enforcement and Declaratory Order; Exelon Wind 1, LLC; Exelon Wind 2, LLC...

Federal Register 2010, 2011, 2012, 2013, 2014

2012-07-10

... Petition for Enforcement and Declaratory Order; Exelon Wind 1, LLC; Exelon Wind 2, LLC; Exelon Wind 3, LLC; Exelon Wind 4, LLC; Exelon Wind 5, LLC; Exelon Wind 6, LLC; Exelon Wind 7, LLC; Exelon Wind 8, LLC; Exelon Wind 9, LLC; Exelon Wind 10, LLC; Exelon Wind 11, LLC; High Plains Wind Power, LLC Take notice...

Inhibition of Intracellular ROS Accumulation by Formononetin Attenuates Cisplatin-Mediated Apoptosis in LLC-PK1 Cells

PubMed Central

Lee, Haesol; Lee, Dahae; Kang, Ki Sung; Song, Ji Hoon; Choi, You-Kyoung

2018-01-01

Cisplatin is a well-known anticancer drug frequently used for treating solid tumors, including ovarian, testicular, bladder, and cervical tumors. However, usage of cisplatin has been limited because of its adverse effects, particularly nephrotoxicity. Therefore, the present study sought to investigate the protective effect of formononetin against cisplatin-induced cytotoxicity in LLC-PK1 pig kidney epithelial cells as well as the anticancer effect of cisplatin in three different human cervical cancer cell lines, including HeLa, SiHa, and CaSKi cells. We first demonstrated that formononetin strongly prevented cisplatin-induced LLC-PK1 cell death. Although formononetin had no anticancer effect, it did not interrupt the anticancer effect of cisplatin in human cervical carcinoma cell lines. Furthermore, the treatment with formononetin reduced reactive oxygen species (ROS) accumulation and chromatin condensation. The percentage of Annexin V-positive cells also increased following cisplatin treatment. Finally, formononetin-inhibited c-Jun N-terminal kinase (JNK) phosphorylation, cleavage of caspase-8 and caspase-3, and the ratio of Bax to Bcl-2 increased with cisplatin. Taken together, these findings suggest that formononetin may be a possible option to prevent nephrotoxicity induced by cisplatin during treatment for cervical cancer. PMID:29534504

Inhibition of Intracellular ROS Accumulation by Formononetin Attenuates Cisplatin-Mediated Apoptosis in LLC-PK1 Cells.

PubMed

Lee, Haesol; Lee, Dahae; Kang, Ki Sung; Song, Ji Hoon; Choi, You-Kyoung

2018-03-12

Cisplatin is a well-known anticancer drug frequently used for treating solid tumors, including ovarian, testicular, bladder, and cervical tumors. However, usage of cisplatin has been limited because of its adverse effects, particularly nephrotoxicity. Therefore, the present study sought to investigate the protective effect of formononetin against cisplatin-induced cytotoxicity in LLC-PK1 pig kidney epithelial cells as well as the anticancer effect of cisplatin in three different human cervical cancer cell lines, including HeLa, SiHa, and CaSKi cells. We first demonstrated that formononetin strongly prevented cisplatin-induced LLC-PK1 cell death. Although formononetin had no anticancer effect, it did not interrupt the anticancer effect of cisplatin in human cervical carcinoma cell lines. Furthermore, the treatment with formononetin reduced reactive oxygen species (ROS) accumulation and chromatin condensation. The percentage of Annexin V-positive cells also increased following cisplatin treatment. Finally, formononetin-inhibited c-Jun N-terminal kinase (JNK) phosphorylation, cleavage of caspase-8 and caspase-3, and the ratio of Bax to Bcl-2 increased with cisplatin. Taken together, these findings suggest that formononetin may be a possible option to prevent nephrotoxicity induced by cisplatin during treatment for cervical cancer.

78 FR 29364 - Exelon Corporation, Exelon Wind 1, LLC, Exelon Wind 2, LLC, Exelon Wind 3, LLC, Exelon Wind 4...

Federal Register 2010, 2011, 2012, 2013, 2014

2013-05-20

...-005, QF07-55-005, QF07-56-005, QF07-257-004] Exelon Corporation, Exelon Wind 1, LLC, Exelon Wind 2, LLC, Exelon Wind 3, LLC, Exelon Wind 4, LLC, Exelon Wind 5, LLC, Exelon Wind 6, LLC, Exelon Wind 7, LLC, Exelon Wind 8, LLC, Exelon Wind 9, LLC, Exelon Wind 10, LLC, Exelon Wind 11, LLC, High Plains...

75 FR 59247 - Combined Notice of Filings #1

Federal Register 2010, 2011, 2012, 2013, 2014

2010-09-27

... Segundo Power II LLC, Long Beach Generation LLC, Long Beach Peakers LLC, NRG Power Marketing Inc., Cabrillo Power I LLC, Cabrillo Power II LLC, El Segundo Power LLC, NRG Solar Blythe LLC, Saguaro Power... LLC, Dunkirk Power LLC, GenConn Energy LLC, GenCon Devon LLC, GenConn Middletown LLC, Huntley Power...

75 FR 6652 - Combined Notice of Filings #1

Federal Register 2010, 2011, 2012, 2013, 2014

2010-02-10

...-004. Applicants: Bendwind, LLC; Big Sky Wind, LLC; DeGreeff DP, LLC; DeGreeffpa, LLC; CL Power Sales... Wind, LLC; EME Homer City Generation, L.P.; Forward WindPower, LLC; Groen Wind, LLC; High Lonesome Mesa, LLC; Hillcrest Wind, LLC; Jeffers Wind 20, LLC; Larswind, LLC; Lookout WindPower, LLC; Midway-Sunset...

77 FR 66457 - Combined Notice of Filings #2

Federal Register 2010, 2011, 2012, 2013, 2014

2012-11-05

..., Casselman Windpower LLC, Colorado Green Holdings LLC, Dillon Wind LLC, Dry Lake Wind Power, LLC, Dry Lake Wind Power II LLC, Elk River Windfarm, LLC, Elm Creek Wind, LLC, Elm Creek Wind II LLC, Farmers City Wind, LLC, Flat Rock Windpower LLC, Flat Rock Windpower II LLC, Flying Cloud Power Partners, LLC...

77 FR 9914 - Combined Notice of Filings #2

Federal Register 2010, 2011, 2012, 2013, 2014

2012-02-21

... Power, LLC, Dry Lake Wind Power II LLC, Elk River Windfarm, LLC, Elm Creek Wind, LLC, Elm Creek Wind II LLC, Farmers City Wind, LLC, Flat Rock Windpower LLC, Flat Rock Windpower II LLC, Flying Cloud Power Partners, LLC, Hardscrabble Wind Power LLC, Hay Canyon Wind LLC, Juniper Canyon Wind Power LLC, Klamath...

78 FR 18580 - Combined Notice of Filings #1

Federal Register 2010, 2011, 2012, 2013, 2014

2013-03-27

.... Applicants: Ashtabula Wind, LLC, Ashtabula Wind II, LLC, Ashtabula Wind III, LLC, Backbone Mountain Windpower LLC, Badger Windpower, LLC, Baldwin Wind, LLC, Bayswater Peaking Facility, LLC, Blackwell Wind, LLC, Butler Ridge Wind Energy Center, LLC, Cimarron Wind Energy, LLC, Crystal Lake Wind, LLC, Crystal Lake...

78 FR 76609 - Genesis Solar, LLC; NRG Delta LLC; Mountain View Solar, LLC; Pheasant Run Wind, LLC; Pheasant Run...

Federal Register 2010, 2011, 2012, 2013, 2014

2013-12-18

... Delta LLC; Mountain View Solar, LLC; Pheasant Run Wind, LLC; Pheasant Run Wind II, LLC; Tuscola Wind II, LLC; Mountain Wind Power, LLC; Mountain Wind Power II, LLC; Summerhaven Wind, LP; Notice of...

75 FR 32455 - Combined Notice of Filings #1

Federal Register 2010, 2011, 2012, 2013, 2014

2010-06-08

... Funding IV, L.L.C., Utility Contract Funding, L.L.C., Vineland Energy LLC, Central Power & Lime LLC, Cedar... Brakes I, L.L.C., Utility Contract Funding, L.L.C., Vineland Energy LLC, Central Power & Lime LLC, Cedar..., L.L.C., Vineland Energy LLC, Central Power & Lime LLC, Cedar Brakes II, L.L.C., J.P. Morgan...

Vectorial transport of unconjugated and conjugated bile salts by monolayers of LLC-PK1 cells doubly transfected with human NTCP and BSEP or with rat Ntcp and Bsep.

PubMed

Mita, Sachiko; Suzuki, Hiroshi; Akita, Hidetaka; Hayashi, Hisamitsu; Onuki, Reiko; Hofmann, Alan F; Sugiyama, Yuichi

2006-03-01

Na(+)-taurocholate-cotransporting peptide (NTCP)/SLC10A1 and bile salt export pump (BSEP)/ABCB11 synergistically play an important role in the transport of bile salts by the hepatocyte. In this study, we transfected human NTCP and BSEP or rat Ntcp and Bsep into LLC-PK1 cells, a cell line devoid of bile salts transporters. Transport by these cells was characterized with a focus on substrate specificity between rats and humans. The basal to apical flux of taurocholate across NTCP- and BSEP-expressing LLC-PK1 monolayers was 10 times higher than that in the opposite direction, whereas the flux across the monolayer of control and NTCP or BSEP single-expressing cells did not show any vectorial transport. The basal to apical flux of taurocholate was saturated with a K(m) value of 20 microM. Vectorial transcellular transport was also observed for cholate, chenodeoxycholate, ursodeoxycholate, their taurine and glycine conjugates, and taurodeoxycholate and glycodeoxycholate, whereas no transport of lithocholate was detected. To evaluate the respective functions of NTCP and BSEP and to compare them with those of rat Ntcp and Bsep, we calculated the clearance by each transporter in this system. A good correlation in the clearance of the examined bile salts (cholate, chenodeoxycholate, ursodeoxycholate, and their taurine or glycine conjugates) was observed between transport by human and that of rat transporters in terms of their rank order: for NTCP, taurine conjugates > glycine conjugates > unconjugated bile salts, and for BSEP, unconjugated bile salts and glycine conjugates > taurine conjugates. In conclusion, the substrate specificity of human and rat NTCP and BSEP appear to be very similar at least for monovalent bile salts under physiological conditions.

77 FR 67356 - Combined Notice of Filings #1

Federal Register 2010, 2011, 2012, 2013, 2014

2012-11-09

..., LLC, FPL Energy Oklahoma Wind, LLC, FPL Energy Sooner Wind, LLC, Minco Wind Interconnection Services, LLC, Minco Wind, LLC, Minco Wind II, LLC, NextEra Energy Power Marketing, LLC. Description... Energy Center, LLC, Crystal Lake Wind, LLC, Crystal Lake Wind II, LLC, Crystal Lake Wind III, LLC, Day...

76 FR 76397 - Combined Notice of Filings #1

Federal Register 2010, 2011, 2012, 2013, 2014

2011-12-07

..., Dry Lake Wind Power, LLC, Dry Lake Wind Power II LLC, Elk River Windfarm, LLC, Elm Creek Wind, LLC..., Flying Cloud Power Partners, LLC, Hardscrabble Wind Power LLC, Hay Canyon Wind LLC, Juniper Canyon Wind Power LLC, Klamath Energy LLC, Klamath Generation LLC, Klondike Wind Power LLC, Klondike Wind Power II...

76 FR 46284 - Combined Notice of Filings #1

Federal Register 2010, 2011, 2012, 2013, 2014

2011-08-02

... Holdings LLC, Dillon Wind LLC, Dry Lake Wind Power, LLC, Dry Lake Wind Power II LLC, Elk River Windfarm... Rock Windpower II LLC, Flying Cloud Power Partners, LLC, Hardscrabble Wind Power LLC, Hay Canyon Wind LLC, Juniper Canyon Wind Power LLC, Klamath Energy LLC, Klamath Generation LLC, Klondike Wind Power...

77 FR 37395 - Combined Notice of Filings #2

Federal Register 2010, 2011, 2012, 2013, 2014

2012-06-21

... Wind Power Partners, LLC, High Prairie Wind Farm II, LLC, Cloud County Wind Farm, LLC, Pioneer Prairie Wind Farm I, LLC, Sagebrush Power Partners, LLC, Arlington Wind Power Project LLC, Marble River, LLC... Power Project LLC, Blue Canyon Windpower II, LLC, Lost Lakes Wind Farm LLC, Blue Canyon Windpower V LLC...

Attenuation of the DNA Damage Response by Transforming Growth Factor-Beta Inhibitors Enhances Radiation Sensitivity of Non–Small-Cell Lung Cancer Cells In Vitro and In Vivo

DOE Office of Scientific and Technical Information (OSTI.GOV)

Du, Shisuo; Bouquet, Sophie; Lo, Chen-Hao

2015-01-01

Purpose: To determine whether transforming growth factor (TGF)-β inhibition increases the response to radiation therapy in human and mouse non–small-cell lung carcinoma (NSCLC) cells in vitro and in vivo. Methods and Materials: TGF-β–mediated growth response and pathway activation were examined in human NSCLC NCI-H1299, NCI-H292, and A549 cell lines and murine Lewis lung cancer (LLC) cells. Cells were treated in vitro with LY364947, a small-molecule inhibitor of the TGF-β type 1 receptor kinase, or with the pan-isoform TGF-β neutralizing monoclonal antibody 1D11 before radiation exposure. The DNA damage response was assessed by ataxia telangiectasia mutated (ATM) or Trp53 protein phosphorylation, γH2AX foci formation,more » or comet assay in irradiated cells. Radiation sensitivity was determined by clonogenic assay. Mice bearing syngeneic subcutaneous LLC tumors were treated with 5 fractions of 6 Gy and/or neutralizing or control antibody. Results: The NCI-H1299, A549, and LLC NSCLC cell lines pretreated with LY364947 before radiation exposure exhibited compromised DNA damage response, indicated by decreased ATM and p53 phosphorylation, reduced γH2AX foci, and increased radiosensitivity. The NCI-H292 cells were unresponsive. Transforming growth factor-β signaling inhibition in irradiated LLC cells resulted in unresolved DNA damage. Subcutaneous LLC tumors in mice treated with TGF-β neutralizing antibody exhibited fewer γH2AX foci after irradiation and significantly greater tumor growth delay in combination with fractionated radiation. Conclusions: Inhibition of TGF-β before radiation attenuated DNA damage recognition and increased radiosensitivity in most NSCLC cells in vitro and promoted radiation-induced tumor control in vivo. These data support the rationale for concurrent TGF-β inhibition and RT to provide therapeutic benefit in NSCLC.« less

78 FR 2382 - Combined Notice of Filings #2

Federal Register 2010, 2011, 2012, 2013, 2014

2013-01-11

...: Blackwell Wind, LLC, CPV Cimarron Renewable Energy Company, LLC, Elk City Wind, LLC, Elk City II Wind, LLC, Ensign Wind, LLC, FPL Energy Cowboy Wind, LLC, FPL Energy Oklahoma Wind, LLC, FPL Energy Sooner Wind, LLC, Gray County Wind Energy, LLC, High Majestic Wind Energy Center, LLC, High Majestic Wind II, LLC, Minco...

77 FR 48138 - Topaz Solar Farms LLC; High Plains Ranch II, LLC; Bethel Wind Energy LLC; Rippey Wind Energy LLC...

Federal Register 2010, 2011, 2012, 2013, 2014

2012-08-13

... Ranch II, LLC; Bethel Wind Energy LLC; Rippey Wind Energy LLC; Pacific Wind, LLC; Colorado Highlands Wind, LLC; Shooting Star Wind Project, LLC; Notice of Effectiveness of Exempt Wholesale Generator or...

Cooperative gene regulation by microRNA pairs and their identification using a computational workflow

PubMed Central

Schmitz, Ulf; Lai, Xin; Winter, Felix; Wolkenhauer, Olaf; Vera, Julio; Gupta, Shailendra K.

2014-01-01

MicroRNAs (miRNAs) are an integral part of gene regulation at the post-transcriptional level. Recently, it has been shown that pairs of miRNAs can repress the translation of a target mRNA in a cooperative manner, which leads to an enhanced effectiveness and specificity in target repression. However, it remains unclear which miRNA pairs can synergize and which genes are target of cooperative miRNA regulation. In this paper, we present a computational workflow for the prediction and analysis of cooperating miRNAs and their mutual target genes, which we refer to as RNA triplexes. The workflow integrates methods of miRNA target prediction; triplex structure analysis; molecular dynamics simulations and mathematical modeling for a reliable prediction of functional RNA triplexes and target repression efficiency. In a case study we analyzed the human genome and identified several thousand targets of cooperative gene regulation. Our results suggest that miRNA cooperativity is a frequent mechanism for an enhanced target repression by pairs of miRNAs facilitating distinctive and fine-tuned target gene expression patterns. Human RNA triplexes predicted and characterized in this study are organized in a web resource at www.sbi.uni-rostock.de/triplexrna/. PMID:24875477

Preservation of protein fluorescence in embedded human dendritic cells for targeted 3D light and electron microscopy.

PubMed

Höhn, K; Fuchs, J; Fröber, A; Kirmse, R; Glass, B; Anders-Össwein, M; Walther, P; Kräusslich, H-G; Dietrich, C

2015-08-01

In this study, we present a correlative microscopy workflow to combine detailed 3D fluorescence light microscopy data with ultrastructural information gained by 3D focused ion beam assisted scanning electron microscopy. The workflow is based on an optimized high pressure freezing/freeze substitution protocol that preserves good ultrastructural detail along with retaining the fluorescence signal in the resin embedded specimens. Consequently, cellular structures of interest can readily be identified and imaged by state of the art 3D confocal fluorescence microscopy and are precisely referenced with respect to an imprinted coordinate system on the surface of the resin block. This allows precise guidance of the focused ion beam assisted scanning electron microscopy and limits the volume to be imaged to the structure of interest. This, in turn, minimizes the total acquisition time necessary to conduct the time consuming ultrastructural scanning electron microscope imaging while eliminating the risk to miss parts of the target structure. We illustrate the value of this workflow for targeting virus compartments, which are formed in HIV-pulsed mature human dendritic cells. © 2015 The Authors Journal of Microscopy © 2015 Royal Microscopical Society.

77 FR 61597 - Avalon Wind, LLC; Avalon Wind 2, LLC; Catalina Solar, LLC; Catalina Solar 2, LLC; Pacific Wind...

Federal Register 2010, 2011, 2012, 2013, 2014

2012-10-10

... DEPARTMENT OF ENERGY Federal Energy Regulatory Commission [Docket No. EL12-109-000] Avalon Wind, LLC; Avalon Wind 2, LLC; Catalina Solar, LLC; Catalina Solar 2, LLC; Pacific Wind Lessee, LLC; Pacific Wind 2, LLC; Valentine Solar, LLC; EDF Renewable Development, Inc.; Notice of Petition for Declaratory...

78 FR 8121 - Combined Notice of Filings #1

Federal Register 2010, 2011, 2012, 2013, 2014

2013-02-05

... Green Holdings LLC, Dillon Wind LLC, Dry Lake Wind Power, LLC, Dry Lake Wind Power II LLC, Elk River..., Flat Rock Windpower II LLC, Flying Cloud Power Partners, LLC, Groton Wind, LLC, Hardscrabble Wind Power LLC, Hay Canyon Wind LLC, Juniper Canyon Wind Power LLC, Klamath [[Page 8122

75 FR 45623 - Morris Energy Group, LLC v.PSEG Energy Resources & Trade LLC; PSEG Fossil LLC; and PSEG Power LLC...

Federal Register 2010, 2011, 2012, 2013, 2014

2010-08-03

... DEPARTMENT OF ENERGY Federal Energy Regulatory Commission [Docket No. EL10-79-000] Morris Energy Group, LLC v.PSEG Energy Resources & Trade LLC; PSEG Fossil LLC; and PSEG Power LLC; Notice of Complaint...) filed a complaint against PSEG Energy Resources & Trade, LLC, PSEG Fossil LLC and PSEG Power LLC (PSEG...

75 FR 27332 - AER NY-Gen, LLC; Eagle Creek Hydro Power, LLC; Eagle Creek Water Resources, LLC; Eagle Creek Land...

Federal Register 2010, 2011, 2012, 2013, 2014

2010-05-14

... 9690-106] AER NY-Gen, LLC; Eagle Creek Hydro Power, LLC; Eagle Creek Water Resources, LLC; Eagle Creek... Power, LLC, Eagle Creek Water Resources, LLC, and Eagle Creek Land Resources, LLC (transferees) filed an.... Paul Ho, Eagle Creek Hydro Power, LLC, Eagle Creek Water Resources, LLC, and Eagle Creek Land Resources...

77 FR 14838 - General Electric-Hitachi Global Laser Enrichment LLC, Commercial Laser-Based Uranium Enrichment...

Federal Register 2010, 2011, 2012, 2013, 2014

2012-03-13

... safety, chemical process safety, fire safety, emergency management, environmental protection... the transportation of SNM of low strategic significance, human factors engineering, and electrical...

Cytotoxic activity of quassinoids from Eurycoma longifolia.

PubMed

Miyake, Katsunori; Li, Feng; Tezuka, Yasuhiro; Awale, Suresh; Kadota, Shigetoshi

2010-07-01

Twenty-four quassinoids isolated from Eurycoma longifolia Jack were investigated for their cytotoxicity against a panel of four different cancer cell lines, which includes three murine cell lines [colon 26-L5 carcinoma (colon 26-L5), B16-BL6 melanoma (B16-BL6), Lewis lung carcinoma (LLC)] and a human lung A549 adenocarcinoma (A549) cell line. Among the tested compounds, eurycomalactone (9) displayed the most potent activity against all the tested cell lines; colon 26-L5 (IC50 = 0.70 microM), B16-BL6 (IC50 = 0.59 microM), LLC (IC50 = 0.78 microM), and A549 (IC50 = 0.73 microM). These activities were comparable to clinically used anticancer agent doxorubicin (colon 26-L5, IC50 = 0.76 microM; B16-BL6, IC50 = 0.86 microM; LLC, IC50 = 0.80 microM; A549, IC50 = 0.66 microM).

Integration and Visualization of Translational Medicine Data for Better Understanding of Human Diseases

PubMed Central

Satagopam, Venkata; Gu, Wei; Eifes, Serge; Gawron, Piotr; Ostaszewski, Marek; Gebel, Stephan; Barbosa-Silva, Adriano; Balling, Rudi; Schneider, Reinhard

2016-01-01

Abstract Translational medicine is a domain turning results of basic life science research into new tools and methods in a clinical environment, for example, as new diagnostics or therapies. Nowadays, the process of translation is supported by large amounts of heterogeneous data ranging from medical data to a whole range of -omics data. It is not only a great opportunity but also a great challenge, as translational medicine big data is difficult to integrate and analyze, and requires the involvement of biomedical experts for the data processing. We show here that visualization and interoperable workflows, combining multiple complex steps, can address at least parts of the challenge. In this article, we present an integrated workflow for exploring, analysis, and interpretation of translational medicine data in the context of human health. Three Web services—tranSMART, a Galaxy Server, and a MINERVA platform—are combined into one big data pipeline. Native visualization capabilities enable the biomedical experts to get a comprehensive overview and control over separate steps of the workflow. The capabilities of tranSMART enable a flexible filtering of multidimensional integrated data sets to create subsets suitable for downstream processing. A Galaxy Server offers visually aided construction of analytical pipelines, with the use of existing or custom components. A MINERVA platform supports the exploration of health and disease-related mechanisms in a contextualized analytical visualization system. We demonstrate the utility of our workflow by illustrating its subsequent steps using an existing data set, for which we propose a filtering scheme, an analytical pipeline, and a corresponding visualization of analytical results. The workflow is available as a sandbox environment, where readers can work with the described setup themselves. Overall, our work shows how visualization and interfacing of big data processing services facilitate exploration, analysis, and interpretation of translational medicine data. PMID:27441714

78 FR 8504 - Combined Notice of Filings #1

Federal Register 2010, 2011, 2012, 2013, 2014

2013-02-06

... II Wind Farm LLC, Fowler Ridge III Wind Farm LLC, Fowler Ridge Wind Farm LLC, Goshen Phase II LLC, Long Island Solar Farm LLC, Mehoopany Wind Energy LLC, Rolling Thunder I Power Partners, LLC, Watson... Farm LLC, Burley Butte Wind Park, LLC, Camp Reed Wind Park, LLC, Golden Valley Wind Park, LL,; Milner...

77 FR 27040 - Combined Notice of Filings #1

Federal Register 2010, 2011, 2012, 2013, 2014

2012-05-08

... Farm LLC, Blackstone Wind Farm II LLC, High Trail Wind Farm, LLC, Meadow Lake Wind Farm LLC, Meadow Lake Wind Farm II LLC, Meadow Lake Wind Farm III LLC, Meadow Lake Wind Farm IV LLC, Old Trail Wind Farm, LLC, and Paulding Wind Farm II LLC. Description: Land Acquisition Report of Blackstone Wind Farm LLC...

77 FR 42714 - Eagle Creek Hydropower, LLC, Eagle Creek Land Resources, LLC, Eagle Creek Water Resources, LLC...

Federal Register 2010, 2011, 2012, 2013, 2014

2012-07-20

... Hydropower, LLC, Eagle Creek Land Resources, LLC, Eagle Creek Water Resources, LLC; Notice of Application...: Eagle Creek Hydropower, LLC; Eagle Creek Land Resources, LLC; and Eagle Creek Water Resources, LLC. e... Contact: Robert Gates, Senior Vice President-- Operations, Eagle Creek Hydropower, LLC, Eagle Creek Water...

77 FR 30272 - Combined Notice of Filings #2

Federal Register 2010, 2011, 2012, 2013, 2014

2012-05-22

... Capital Holdings, LLC, Harvest WindFarm, LLC, CER Generation II, LLC, Handsome Lake Energy, LLC, Exelon..., LLC, Exelon Wyman, LLC, Exelon Generation Company, LLC, Exelon Energy Company, CER Generation, LLC...

78 FR 7425 - Combined Notice of Filings #1

Federal Register 2010, 2011, 2012, 2013, 2014

2013-02-01

... Allegheny LLC, BE CA LLC, BE Ironwood LLC, BE KJ LLC, BE Louisiana LLC, BE Rayle LLC, Cedar Brakes I, L.L.C., Cedar Brakes II, L.L.C., Central Power & Lime LLC, Triton Power Michigan LLC, Utility Contract Funding... that the Commission received the following electric rate filings: Docket Numbers: ER10-1707-002; ER11...

76 FR 76153 - Notice of Effectiveness of Exempt Wholesale Generator Status; Caney River Wind Project, LLC...

Federal Register 2010, 2011, 2012, 2013, 2014

2011-12-06

...] Notice of Effectiveness of Exempt Wholesale Generator Status; Caney River Wind Project, LLC, Mesquite Solar 1, LLC, Copper Crossing Solar LLC, Copper Mountain Solar 1, LLC, Pinnacle Wind, LLC, Bellevue Solar, LLC, Yamhill Solar, LLC, Osage Wind, LLC, Minco Wind II, LLC Take notice that during the month of...

75 FR 18496 - Notice of Complaint Filing

Federal Register 2010, 2011, 2012, 2013, 2014

2010-04-12

... marketing LLC, Connecticut Jet Power LLC, Devon Power LLC, Middletown Power LLC, Montville Power LLC... Resources & Trade, LLC, PSEG Power Connecticut LLC, NRG Power marketing LLC, Connecticut Jet Power LLC... subscribed docket(s). For assistance with any FERC Online service, please e-mail [email protected

Domain-Adapted Convolutional Networks for Satellite Image Classification: A Large-Scale Interactive Learning Workflow

DOE PAGES

Lunga, Dalton D.; Yang, Hsiuhan Lexie; Reith, Andrew E.; ...

2018-02-06

Satellite imagery often exhibits large spatial extent areas that encompass object classes with considerable variability. This often limits large-scale model generalization with machine learning algorithms. Notably, acquisition conditions, including dates, sensor position, lighting condition, and sensor types, often translate into class distribution shifts introducing complex nonlinear factors and hamper the potential impact of machine learning classifiers. Here, this article investigates the challenge of exploiting satellite images using convolutional neural networks (CNN) for settlement classification where the class distribution shifts are significant. We present a large-scale human settlement mapping workflow based-off multiple modules to adapt a pretrained CNN to address themore » negative impact of distribution shift on classification performance. To extend a locally trained classifier onto large spatial extents areas we introduce several submodules: First, a human-in-the-loop element for relabeling of misclassified target domain samples to generate representative examples for model adaptation; second, an efficient hashing module to minimize redundancy and noisy samples from the mass-selected examples; and third, a novel relevance ranking module to minimize the dominance of source example on the target domain. The workflow presents a novel and practical approach to achieve large-scale domain adaptation with binary classifiers that are based-off CNN features. Experimental evaluations are conducted on areas of interest that encompass various image characteristics, including multisensors, multitemporal, and multiangular conditions. Domain adaptation is assessed on source–target pairs through the transfer loss and transfer ratio metrics to illustrate the utility of the workflow.« less

Domain-Adapted Convolutional Networks for Satellite Image Classification: A Large-Scale Interactive Learning Workflow

DOE Office of Scientific and Technical Information (OSTI.GOV)

Lunga, Dalton D.; Yang, Hsiuhan Lexie; Reith, Andrew E.

Satellite imagery often exhibits large spatial extent areas that encompass object classes with considerable variability. This often limits large-scale model generalization with machine learning algorithms. Notably, acquisition conditions, including dates, sensor position, lighting condition, and sensor types, often translate into class distribution shifts introducing complex nonlinear factors and hamper the potential impact of machine learning classifiers. Here, this article investigates the challenge of exploiting satellite images using convolutional neural networks (CNN) for settlement classification where the class distribution shifts are significant. We present a large-scale human settlement mapping workflow based-off multiple modules to adapt a pretrained CNN to address themore » negative impact of distribution shift on classification performance. To extend a locally trained classifier onto large spatial extents areas we introduce several submodules: First, a human-in-the-loop element for relabeling of misclassified target domain samples to generate representative examples for model adaptation; second, an efficient hashing module to minimize redundancy and noisy samples from the mass-selected examples; and third, a novel relevance ranking module to minimize the dominance of source example on the target domain. The workflow presents a novel and practical approach to achieve large-scale domain adaptation with binary classifiers that are based-off CNN features. Experimental evaluations are conducted on areas of interest that encompass various image characteristics, including multisensors, multitemporal, and multiangular conditions. Domain adaptation is assessed on source–target pairs through the transfer loss and transfer ratio metrics to illustrate the utility of the workflow.« less

77 FR 13592 - AER NY-Gen, LLC; Eagle Creek Hydro Power, LLC, Eagle Creek Water Resources, LLC, Eagle Creek Land...

Federal Register 2010, 2011, 2012, 2013, 2014

2012-03-07

...; Eagle Creek Hydro Power, LLC, Eagle Creek Water Resources, LLC, Eagle Creek Land Resources, LLC; Notice... 24, 2012, AER NY-Gen, LLC (transferor), Eagle Creek Hydro Power, LLC, Eagle Creek Water Resources.... Cherry, Eagle Creek Hydro Power, LLC, Eagle Creek Water Resources, LLC, and Eagle Creek Land Resources...

76 FR 27037 - Combined Notice of Filings #2

Federal Register 2010, 2011, 2012, 2013, 2014

2011-05-10

..., Invenergy TN LLC, Wolverine Creek Energy LLC, Grays Harbor Energy LLC, Forward Energy LLC, Willow Creek... III LLC, Grand Ridge Energy IV LLC, Grand Ridge Energy V LLC, Vantage Wind Energy LLC, White Oak...

77 FR 41777 - Combined Notice of Filings #1

Federal Register 2010, 2011, 2012, 2013, 2014

2012-07-16

...., Commonwealth Edison Company, PECO Energy Company, Wind Capital Holdings, LLC, Constellation Power Source... Generation II, LLC, Constellation Mystic Power, LLC, Cassia Gulch Wind Park, LLC, Michigan Wind 1, LLC, Tuana Springs Energy, LLC, Harvest Windfarm, LLC, CR Clearing, LLC, Exelon Wind 4, LLC, Cow Branch Wind Power, L...

77 FR 3468 - Combined Notice of Filings #1

Federal Register 2010, 2011, 2012, 2013, 2014

2012-01-24

... Energy LLC, Hardee Power Partners Limited, Judith Gap Energy LLC, Invenergy TN LLC, Wolverine Creek... Energy V LLC, Vantage Wind Energy LLC, Beech Ridge Energy LLC, Gratiot County Wind LLC, Stony Creek Energy LLC, Gratiot County Wind II LLC. Description: Notification of Change in Facts Under Market-Based...

77 FR 59599 - Combined Notice of Filings #1

Federal Register 2010, 2011, 2012, 2013, 2014

2012-09-28

.... Applicants: Dry Lake Wind Power II LLC, Central Maine Power Company, Flat Rock Windpower II LLC, Flat Rock Windpower LLC, Elk River Windfarm, LLC, Iberdrola Renewables, LLC, Dillion Wind LLC, Dry Lake Wind Power, LLC, Shiloh I Wind Project, LLC, Mountain View Power Partners III, LLC, Blue [[Page 59600

Antibody-directed myostatin inhibition enhances muscle mass and function in tumor-bearing mice.

PubMed

Murphy, Kate T; Chee, Annabel; Gleeson, Ben G; Naim, Timur; Swiderski, Kristy; Koopman, René; Lynch, Gordon S

2011-09-01

Cancer cachexia describes the progressive skeletal muscle wasting and weakness in many cancer patients and accounts for >20% of cancer-related deaths. We tested the hypothesis that antibody-directed myostatin inhibition would attenuate the atrophy and loss of function in muscles of tumor-bearing mice. Twelve-week-old C57BL/6 mice received a subcutaneous injection of saline (control) or Lewis lung carcinoma (LLC) tumor cells. One week later, mice received either once weekly injections of saline (control, n = 12; LLC, n = 9) or a mouse chimera of anti-human myostatin antibody (PF-354, 10 mg·kg⁻¹·wk⁻¹, LLC+PF-354, n = 11) for 5 wk. Injection of LLC cells reduced muscle mass and maximum force of tibialis anterior (TA) muscles by 8-10% (P < 0.05), but the muscle atrophy and weakness were prevented with PF-354 treatment (P > 0.05). Maximum specific (normalized) force of diaphragm muscle strips was reduced with LLC injection (P < 0.05) but was not improved with PF-354 treatment (P > 0.05). PF-354 enhanced activity of oxidative enzymes in TA and diaphragm muscles of tumor-bearing mice by 118% and 89%, respectively (P < 0.05). Compared with controls, apoptosis that was not of myofibrillar or satellite cell origin was 140% higher in TA muscle cross sections from saline-treated LLC tumor-bearing mice (P < 0.05) but was not different in PF-354-treated tumor-bearing mice (P > 0.05). Antibody-directed myostatin inhibition attenuated the skeletal muscle atrophy and loss of muscle force-producing capacity in a murine model of cancer cachexia, in part by reducing apoptosis. The improvements in limb muscle mass and function highlight the therapeutic potential of antibody-directed myostatin inhibition for cancer cachexia.

77 FR 34093 - License Renewal for Calvert Cliffs Nuclear Power Plant, LLC's

Federal Register 2010, 2011, 2012, 2013, 2014

2012-06-08

... quality; water quality and use; geology and soils; ecology; noise; historical and cultural; scenic and... significantly affect the quality of the human environment. Therefore, preparation of an environmental impact...

77 FR 14010 - Rocky Ridge Wind Project, LLC, Blackwell Wind, LLC, CPV Cimarron Renewable Energy Company, LLC...

Federal Register 2010, 2011, 2012, 2013, 2014

2012-03-08

..., EG12-18-000, EG12-19-000, EG12- 20-000, EG12-21-000, EG12-22-000, EG12-23-000] Rocky Ridge Wind Project, LLC, Blackwell Wind, LLC, CPV Cimarron Renewable Energy Company, LLC, Minco Wind Interconnection Services, LLC, Shiloh III Lessee, LLC, California Ridge Wind Energy LLC, Perrin Ranch Wind, LLC, Erie Wind...

77 FR 3471 - Combined Notice of Filings #2

Federal Register 2010, 2011, 2012, 2013, 2014

2012-01-24

...: J.P. Morgan Ventures Energy Corporation, Triton Power Michigan LLC, BE Allegheny LLC, BE CA LLC, BE Ironwood LLC, BE KJ LLC, BE Rayle LLC, BE Alabama LLC, BE Louisiana LLC, Cedar Brakes I LLC, Central Power... that the Commission received the following electric rate filings: Docket Numbers: ER10-2319-002; ER10...

77 FR 41397 - Combined Notice of Filings #1

Federal Register 2010, 2011, 2012, 2013, 2014

2012-07-13

...; ER10-2504-002; ER12-610-002; ER10-2436-003; ER11-4381-002. Applicants: Fenton Power Partners I, LLC, Wapsipinicon Wind Project, LLC, Shiloh Wind Project 2, LLC, Hoosier Wind Project, LLC, Oasis Power Partners, LLC, Bellevue Solar, LLC, Yamhill Solar, LLC, Chanarambie Power Partners, LLC, LWP Lessee, LLC...

77 FR 274 - Combined Notice of Filings #1

Federal Register 2010, 2011, 2012, 2013, 2014

2012-01-04

... Capital Holdings, LLC, Cow Branch Wind Power, L.L.C. Description: Updated Market Power Analysis of Exelon..., LLC, AES Alamitos, LLC, AES Redondo Beach, L.L.C., Condon Wind Power, LLC, AES Huntington Beach, L.L.C...-000. Applicants: Erie Wind, LLC. Description: Self-Certification of EWG Status of Erie Wind, LLC...

77 FR 5007 - Combined Notice of Filings #2

Federal Register 2010, 2011, 2012, 2013, 2014

2012-02-01

...; ER11-2488-002; ER10-3032-002; ER11-2475-002. Applicants: Klondike Wind Power III LLC, Northern Iowa... Windpower LLC, Flying Cloud Power Partners, LLC, Klamath Energy LLC, Klamath Generation LLC, Moraine Wind LLC, Mountain View Power Partners III, LLC, Shiloh I Wind Project, LLC, Trimont Wind I LLC, Locust...

75 FR 47301 - Cedro Hill Wind LLC; Butler Ridge Wind Energy Center, LLC; High Majestic Wind Energy Center, LLC...

Federal Register 2010, 2011, 2012, 2013, 2014

2010-08-05

...- 000; EG10-34-000; EG10-34-000; EG10-35-000; EG10-36-000; EG10-37-000; EG10-38-000] Cedro Hill Wind LLC; Butler Ridge Wind Energy Center, LLC; High Majestic Wind Energy Center, LLC; Wessington Wind Energy Center, LLC; Juniper Canyon Wind Power LLC; Loraine Windpark Project, LLC; White Oak Energy LLC; Meadow...

DIaaS: Data-Intensive workflows as a service - Enabling easy composition and deployment of data-intensive workflows on Virtual Research Environments

NASA Astrophysics Data System (ADS)

Filgueira, R.; Ferreira da Silva, R.; Deelman, E.; Atkinson, M.

2016-12-01

We present the Data-Intensive workflows as a Service (DIaaS) model for enabling easy data-intensive workflow composition and deployment on clouds using containers. DIaaS model backbone is Asterism, an integrated solution for running data-intensive stream-based applications on heterogeneous systems, which combines the benefits of dispel4py with Pegasus workflow systems. The stream-based executions of an Asterism workflow are managed by dispel4py, while the data movement between different e-Infrastructures, and the coordination of the application execution are automatically managed by Pegasus. DIaaS combines Asterism framework with Docker containers to provide an integrated, complete, easy-to-use, portable approach to run data-intensive workflows on distributed platforms. Three containers integrate the DIaaS model: a Pegasus node, and an MPI and an Apache Storm clusters. Container images are described as Dockerfiles (available online at http://github.com/dispel4py/pegasus_dispel4py), linked to Docker Hub for providing continuous integration (automated image builds), and image storing and sharing. In this model, all required software (workflow systems and execution engines) for running scientific applications are packed into the containers, which significantly reduces the effort (and possible human errors) required by scientists or VRE administrators to build such systems. The most common use of DIaaS will be to act as a backend of VREs or Scientific Gateways to run data-intensive applications, deploying cloud resources upon request. We have demonstrated the feasibility of DIaaS using the data-intensive seismic ambient noise cross-correlation application (Figure 1). The application preprocesses (Phase1) and cross-correlates (Phase2) traces from several seismic stations. The application is submitted via Pegasus (Container1), and Phase1 and Phase2 are executed in the MPI (Container2) and Storm (Container3) clusters respectively. Although both phases could be executed within the same environment, this setup demonstrates the flexibility of DIaaS to run applications across e-Infrastructures. In summary, DIaaS delivers specialized software to execute data-intensive applications in a scalable, efficient, and robust manner reducing the engineering time and computational cost.

Developing a workflow to identify inconsistencies in volunteered geographic information: a phenological case study

USGS Publications Warehouse

Mehdipoor, Hamed; Zurita-Milla, Raul; Rosemartin, Alyssa; Gerst, Katharine L.; Weltzin, Jake F.

2015-01-01

Recent improvements in online information communication and mobile location-aware technologies have led to the production of large volumes of volunteered geographic information. Widespread, large-scale efforts by volunteers to collect data can inform and drive scientific advances in diverse fields, including ecology and climatology. Traditional workflows to check the quality of such volunteered information can be costly and time consuming as they heavily rely on human interventions. However, identifying factors that can influence data quality, such as inconsistency, is crucial when these data are used in modeling and decision-making frameworks. Recently developed workflows use simple statistical approaches that assume that the majority of the information is consistent. However, this assumption is not generalizable, and ignores underlying geographic and environmental contextual variability that may explain apparent inconsistencies. Here we describe an automated workflow to check inconsistency based on the availability of contextual environmental information for sampling locations. The workflow consists of three steps: (1) dimensionality reduction to facilitate further analysis and interpretation of results, (2) model-based clustering to group observations according to their contextual conditions, and (3) identification of inconsistent observations within each cluster. The workflow was applied to volunteered observations of flowering in common and cloned lilac plants (Syringa vulgaris and Syringa x chinensis) in the United States for the period 1980 to 2013. About 97% of the observations for both common and cloned lilacs were flagged as consistent, indicating that volunteers provided reliable information for this case study. Relative to the original dataset, the exclusion of inconsistent observations changed the apparent rate of change in lilac bloom dates by two days per decade, indicating the importance of inconsistency checking as a key step in data quality assessment for volunteered geographic information. Initiatives that leverage volunteered geographic information can adapt this workflow to improve the quality of their datasets and the robustness of their scientific analyses.

SigWin-detector: a Grid-enabled workflow for discovering enriched windows of genomic features related to DNA sequences.

PubMed

Inda, Márcia A; van Batenburg, Marinus F; Roos, Marco; Belloum, Adam S Z; Vasunin, Dmitry; Wibisono, Adianto; van Kampen, Antoine H C; Breit, Timo M

2008-08-08

Chromosome location is often used as a scaffold to organize genomic information in both the living cell and molecular biological research. Thus, ever-increasing amounts of data about genomic features are stored in public databases and can be readily visualized by genome browsers. To perform in silico experimentation conveniently with this genomics data, biologists need tools to process and compare datasets routinely and explore the obtained results interactively. The complexity of such experimentation requires these tools to be based on an e-Science approach, hence generic, modular, and reusable. A virtual laboratory environment with workflows, workflow management systems, and Grid computation are therefore essential. Here we apply an e-Science approach to develop SigWin-detector, a workflow-based tool that can detect significantly enriched windows of (genomic) features in a (DNA) sequence in a fast and reproducible way. For proof-of-principle, we utilize a biological use case to detect regions of increased and decreased gene expression (RIDGEs and anti-RIDGEs) in human transcriptome maps. We improved the original method for RIDGE detection by replacing the costly step of estimation by random sampling with a faster analytical formula for computing the distribution of the null hypothesis being tested and by developing a new algorithm for computing moving medians. SigWin-detector was developed using the WS-VLAM workflow management system and consists of several reusable modules that are linked together in a basic workflow. The configuration of this basic workflow can be adapted to satisfy the requirements of the specific in silico experiment. As we show with the results from analyses in the biological use case on RIDGEs, SigWin-detector is an efficient and reusable Grid-based tool for discovering windows enriched for features of a particular type in any sequence of values. Thus, SigWin-detector provides the proof-of-principle for the modular e-Science based concept of integrative bioinformatics experimentation.

Automating radiologist workflow, part 3: education and training.

PubMed

Reiner, Bruce

2008-12-01

The current model for radiologist education consists largely of mentorship during residency, followed by peer-to-peer training thereafter. The traditional focus of this radiologist education has historically been restricted to anatomy, pathology, and imaging modality. This "human" mentoring model becomes a limiting factor in the current practice environment because of rapid and dramatic changes in imaging and information technologies, along with the increased time demands placed on practicing radiologists. One novel way to address these burgeoning education and training challenges is to leverage technology, with the creation of user-specific and context-specific automated workflow templates. These automated templates would provide a low-stress, time-efficient, and easy-to-use equivalent of "computerized" mentoring. A radiologist could identify the workflow template of interest on the basis of the specific computer application, pathology, anatomy, or modality of interest. While the corresponding workflow template is activated, the radiologist "student" could effectively start and stop at areas of interest and use the functionality of an electronic wizard to identify additional educational resource of interest. An additional training feature of the technology is the ability to review "proven" cases for the purposes of establishing competence and credentialing.

DOE Office of Scientific and Technical Information (OSTI.GOV)

Aurich, Maike K.; Fleming, Ronan M. T.; Thiele, Ines

Metabolomic data sets provide a direct read-out of cellular phenotypes and are increasingly generated to study biological questions. Previous work, by us and others, revealed the potential of analyzing extracellular metabolomic data in the context of the metabolic model using constraint-based modeling. With the MetaboTools, we make our methods available to the broader scientific community. The MetaboTools consist of a protocol, a toolbox, and tutorials of two use cases. The protocol describes, in a step-wise manner, the workflow of data integration, and computational analysis. The MetaboTools comprise the Matlab code required to complete the workflow described in the protocol. Tutorialsmore » explain the computational steps for integration of two different data sets and demonstrate a comprehensive set of methods for the computational analysis of metabolic models and stratification thereof into different phenotypes. The presented workflow supports integrative analysis of multiple omics data sets. Importantly, all analysis tools can be applied to metabolic models without performing the entire workflow. Taken together, the MetaboTools constitute a comprehensive guide to the intra-model analysis of extracellular metabolomic data from microbial, plant, or human cells. In conclusion, this computational modeling resource offers a broad set of computational analysis tools for a wide biomedical and non-biomedical research community.« less

78 FR 9904 - Combined Notice of Filings #1

Federal Register 2010, 2011, 2012, 2013, 2014

2013-02-12

... DEPARTMENT OF ENERGY Federal Energy Regulatory Commission Combined Notice of Filings 1 Take notice..., LLC, Bayswater Peaking Facility, LLC, Blackwell Wind, LLC, Butler Ridge Wind Energy Center, LLC, Cimarron Wind Energy, LLC, Crystal Lake Wind, LLC, Crystal Lake Wind II, LLC, Crystal Lake Wind III, LLC...

78 FR 26023 - Combined Notice of Filings #1

Federal Register 2010, 2011, 2012, 2013, 2014

2013-05-03

... DEPARTMENT OF ENERGY Federal Energy Regulatory Commission Combined Notice of Filings 1 Take notice... I LLC, Cabrillo Power II LLC, Conemaugh Power LLC, Connecticut Jet Power LLC, Cottonwood Energy Company, LP, Devon Power LLC, Dunkirk Power LLC, EL Segundo Energy Center LLC, El Segundo Power, LLC...

77 FR 27046 - Combined Notice of Filings #2

Federal Register 2010, 2011, 2012, 2013, 2014

2012-05-08

..., Copper Mountain Solar 1, LLC, El Dorado Energy, LLC, Fowler Ridge II Wind Farm LLC, Mesquite Power, LLC..., AES Redondo Beach, LLC, Condon Wind Power, LLC, Indianapolis Power & Light Company, Mountain View... Sterling CT Limited Partnership, Bayonne Plant Holding, LLC, Camden Plant Holding, LLC, Dartmouth Power...

77 FR 58120 - Combined Notice of Filings #2

Federal Register 2010, 2011, 2012, 2013, 2014

2012-09-19

.... Applicants: Constellation Energy Commodities Group, Inc., R.E. Ginna Nuclear Power Plant, LLC, PECO Energy... Point Nuclear Station, LLC, Constellation Mystic Power, LLC, Cassia Gulch Wind Park, LLC, Michigan Wind 1, LLC, Harvest Windfarm, LLC, Exelon Wind 4, LLC, Criterion Power Partners, LLC, Cow Branch Wind...

78 FR 39724 - Combined Notice of Filings #2

Federal Register 2010, 2011, 2012, 2013, 2014

2013-07-02

... Trading North America, LLC, EDF Industrial Power Services (NY), LLC, EDF Industrial Power Services (IL), LLC, EDF Industrial Power Services (CA), LLC, Tanner Street Generation, LLC. Description: Notice of...: AP Holdings, LLC, AP Gas & Electric (PA), LLC, AP Gas & Electric (TX), LLC, AP Gas & Electric (MD...

Performances of the PIPER scalable child human body model in accident reconstruction

PubMed Central

Giordano, Chiara; Kleiven, Svein

2017-01-01

Human body models (HBMs) have the potential to provide significant insights into the pediatric response to impact. This study describes a scalable/posable approach to perform child accident reconstructions using the Position and Personalize Advanced Human Body Models for Injury Prediction (PIPER) scalable child HBM of different ages and in different positions obtained by the PIPER tool. Overall, the PIPER scalable child HBM managed reasonably well to predict the injury severity and location of the children involved in real-life crash scenarios documented in the medical records. The developed methodology and workflow is essential for future work to determine child injury tolerances based on the full Child Advanced Safety Project for European Roads (CASPER) accident reconstruction database. With the workflow presented in this study, the open-source PIPER scalable HBM combined with the PIPER tool is also foreseen to have implications for improved safety designs for a better protection of children in traffic accidents. PMID:29135997

77 FR 6105 - Combined Notice of Filings #1

Federal Register 2010, 2011, 2012, 2013, 2014

2012-02-07

... River LLC, Somerset Windpower, LLC, Story Wind, LLC, Vasco Winds, LLC, Victory Garden Phase IV, LLC...: East Coast Power Linden Holding, LLC, Cogen Technologies Linden Venture, L.P., Fox Energy Company, LLC...

78 FR 54487 - YP Western Directory LLC, San Francisco Division, Publishing Operations Group, YP Subsidiary...

Federal Register 2010, 2011, 2012, 2013, 2014

2013-09-04

... Directory LLC, San Francisco Division, Publishing Operations Group, YP Subsidiary Holdings LLC, YP LLC, YP... Directory LLC, San Francisco Division, Publishing Operations Group, YP Subsidiary Holdings LLC, YP LLC, YP... workers of YP Western Directory LLC, San Francisco Division, Publishing Operations Group, YP Subsidiary...

78 FR 49496 - Combined Notice of Filings #2

Federal Register 2010, 2011, 2012, 2013, 2014

2013-08-14

... LLC, Vantage Wind Energy LLC, Stony Creek Energy LLC, Gratiot County Wind LLC, Gratiot County Wind II LLC, Bishop Hill Energy LLC, Bishop Hill Energy III LLC, California Ridge Wind Energy LLC. Description.... Docket Numbers: ER13-2056-000. Applicants: Southwest Power Pool, Inc. Description: 2198R9 Kansas Power...

75 FR 7305 - Drake Switching Company, LLC-Operation Exemption- Drake Cement, LLC

Federal Register 2010, 2011, 2012, 2013, 2014

2010-02-18

... Switching Company, LLC--Operation Exemption-- Drake Cement, LLC Drake Switching Company, LLC (DSC), a... and operated by Drake Cement, LLC.\\1\\ \\1\\ Drake Cement, LLC filed a verified notice of exemption to acquire these track in STB Finance Docket No. 35350, Drake Cement, LLC--Acquisition Exemption--Clarkdale...

76 FR 36914 - Astoria Generating Company, L.P., NRG Power Marketing LLC, Arthur Kill Power LLC, Astoria Gas...

Federal Register 2010, 2011, 2012, 2013, 2014

2011-06-23

... DEPARTMENT OF ENERGY Federal Energy Regulatory Commission [Docket No. EL11-42-000] Astoria Generating Company, L.P., NRG Power Marketing LLC, Arthur Kill Power LLC, Astoria Gas Turbine Power LLC, Dunkirk Power LLC, Huntley Power LLC, Oswego Harbor Power LLC, TC Ravenswood, LLC, v. New York Independent System Operator, Inc. Notice of Revised...

76 FR 34692 - Astoria Generating Company, L.P., NRG Power Marketing LLC, Arthur Kill Power LLC, Astoria Gas...

Federal Register 2010, 2011, 2012, 2013, 2014

2011-06-14

... DEPARTMENT OF ENERGY Federal Energy Regulatory Commission [Docket No. EL11-42-000] Astoria Generating Company, L.P., NRG Power Marketing LLC, Arthur Kill Power LLC, Astoria Gas Turbine Power LLC, Dunkirk Power LLC, Huntley Power LLC, Oswego Harbor Power LLC, TC Ravenswood, LLC; v. New York Independent System Operator, Inc.; Notice of Complaint...

76 FR 36910 - Astoria Generating Company, L.P., NRG Power Marketing LLC, Arthur Kill Power LLC, Astoria Gas...

Federal Register 2010, 2011, 2012, 2013, 2014

2011-06-23

... DEPARTMENT OF ENERGY Federal Energy Regulatory Commission [Docket No. EL11-42-000] Astoria Generating Company, L.P., NRG Power Marketing LLC, Arthur Kill Power LLC, Astoria Gas Turbine Power LLC, Dunkirk Power LLC, Huntley Power LLC, Oswego Harbor Power LLC, TC Ravenswood, LLC. v. New York Independent System Operator, Inc.; Notice of Amendment t...

75 FR 1052 - Terra-Gen Dixie Valley, LLC; TGP Dixie Development Company, LLC; New York Canyon, LLC; Notice of...

Federal Register 2010, 2011, 2012, 2013, 2014

2010-01-08

... DEPARTMENT OF ENERGY Federal Energy Regulatory Commission [Docket No. EL10-29-000] Terra-Gen Dixie Valley, LLC; TGP Dixie Development Company, LLC; New York Canyon, LLC; Notice of Filing December 30, 2009. Take notice that on December 24, 2009, Terra-Gen Dixie Valley, LLC, TGP Dixie Development Company, LLC and New York Canyon, LLC (``Petitioner...

78 FR 49498 - Combined Notice of Filings #1

Federal Register 2010, 2011, 2012, 2013, 2014

2013-08-14

..., Day County Wind, LLC, Diablo Winds, LLC, Desert Sunlight 250, LLC, Desert Sunlight 300, LLC, Elk City Wind, LLC, Elk City II Wind, LLC, Ensign Wind, LLC, ESI Vansycle Partners, L.P., Florida Power & Light...

Multi-level meta-workflows: new concept for regularly occurring tasks in quantum chemistry.

PubMed

Arshad, Junaid; Hoffmann, Alexander; Gesing, Sandra; Grunzke, Richard; Krüger, Jens; Kiss, Tamas; Herres-Pawlis, Sonja; Terstyanszky, Gabor

2016-01-01

In Quantum Chemistry, many tasks are reoccurring frequently, e.g. geometry optimizations, benchmarking series etc. Here, workflows can help to reduce the time of manual job definition and output extraction. These workflows are executed on computing infrastructures and may require large computing and data resources. Scientific workflows hide these infrastructures and the resources needed to run them. It requires significant efforts and specific expertise to design, implement and test these workflows. Many of these workflows are complex and monolithic entities that can be used for particular scientific experiments. Hence, their modification is not straightforward and it makes almost impossible to share them. To address these issues we propose developing atomic workflows and embedding them in meta-workflows. Atomic workflows deliver a well-defined research domain specific function. Publishing workflows in repositories enables workflow sharing inside and/or among scientific communities. We formally specify atomic and meta-workflows in order to define data structures to be used in repositories for uploading and sharing them. Additionally, we present a formal description focused at orchestration of atomic workflows into meta-workflows. We investigated the operations that represent basic functionalities in Quantum Chemistry, developed the relevant atomic workflows and combined them into meta-workflows. Having these workflows we defined the structure of the Quantum Chemistry workflow library and uploaded these workflows in the SHIWA Workflow Repository.Graphical AbstractMeta-workflows and embedded workflows in the template representation.

78 FR 67138 - Combined Notice of Filings #1

Federal Register 2010, 2011, 2012, 2013, 2014

2013-11-08

... Solar 1, LLC, Copper Mountain Solar 2, LLC, Energia Sierra Juarez U.S., LLC, Flat Ridge 2 Wind Energy LLC, Fowler Ridge II Wind Farm LLC, Mehoopany Wind Energy LLC, Mesquite Power, LLC, Mesquite Solar 1...

SHIWA Services for Workflow Creation and Sharing in Hydrometeorolog

NASA Astrophysics Data System (ADS)

Terstyanszky, Gabor; Kiss, Tamas; Kacsuk, Peter; Sipos, Gergely

2014-05-01

Researchers want to run scientific experiments on Distributed Computing Infrastructures (DCI) to access large pools of resources and services. To run these experiments requires specific expertise that they may not have. Workflows can hide resources and services as a virtualisation layer providing a user interface that researchers can use. There are many scientific workflow systems but they are not interoperable. To learn a workflow system and create workflows may require significant efforts. Considering these efforts it is not reasonable to expect that researchers will learn new workflow systems if they want to run workflows developed in other workflow systems. To overcome it requires creating workflow interoperability solutions to allow workflow sharing. The FP7 'Sharing Interoperable Workflow for Large-Scale Scientific Simulation on Available DCIs' (SHIWA) project developed the Coarse-Grained Interoperability concept (CGI). It enables recycling and sharing workflows of different workflow systems and executing them on different DCIs. SHIWA developed the SHIWA Simulation Platform (SSP) to implement the CGI concept integrating three major components: the SHIWA Science Gateway, the workflow engines supported by the CGI concept and DCI resources where workflows are executed. The science gateway contains a portal, a submission service, a workflow repository and a proxy server to support the whole workflow life-cycle. The SHIWA Portal allows workflow creation, configuration, execution and monitoring through a Graphical User Interface using the WS-PGRADE workflow system as the host workflow system. The SHIWA Repository stores the formal description of workflows and workflow engines plus executables and data needed to execute them. It offers a wide-range of browse and search operations. To support non-native workflow execution the SHIWA Submission Service imports the workflow and workflow engine from the SHIWA Repository. This service either invokes locally or remotely pre-deployed workflow engines or submits workflow engines with the workflow to local or remote resources to execute workflows. The SHIWA Proxy Server manages certificates needed to execute the workflows on different DCIs. Currently SSP supports sharing of ASKALON, Galaxy, GWES, Kepler, LONI Pipeline, MOTEUR, Pegasus, P-GRADE, ProActive, Triana, Taverna and WS-PGRADE workflows. Further workflow systems can be added to the simulation platform as required by research communities. The FP7 'Building a European Research Community through Interoperable Workflows and Data' (ER-flow) project disseminates the achievements of the SHIWA project to build workflow user communities across Europe. ER-flow provides application supports to research communities within (Astrophysics, Computational Chemistry, Heliophysics and Life Sciences) and beyond (Hydrometeorology and Seismology) to develop, share and run workflows through the simulation platform. The simulation platform supports four usage scenarios: creating and publishing workflows in the repository, searching and selecting workflows in the repository, executing non-native workflows and creating and running meta-workflows. The presentation will outline the CGI concept, the SHIWA Simulation Platform, the ER-flow usage scenarios and how the Hydrometeorology research community runs simulations on SSP.

78 FR 9907 - TGP Development Company, LLC; TGP Flying Cloud Holdings, LLC; WEC TX Company, LLC v. Arizona...

Federal Register 2010, 2011, 2012, 2013, 2014

2013-02-12

... DEPARTMENT OF ENERGY Federal Energy Regulatory Commission [Docket No. EL13-45-000] TGP Development Company, LLC; TGP Flying Cloud Holdings, LLC; WEC TX Company, LLC v. Arizona Public Service Company... Cloud Holdings, LLC, and WEC TX Company, LLC (collectively, Complainants) filed a formal complaint...

76 FR 69252 - Combined Notice of Filings #1

Federal Register 2010, 2011, 2012, 2013, 2014

2011-11-08

..., Butler Ridge Wind Energy Center, LLC, Calhoun Power Company I, LLC, Crystal Lake Wind, LLC, Crystal Lake... Partnership, Elk City Wind, LLC, Elk City II Wind, LLC, ESI Vansycle Partners, L.P., Florida Power & Light Co... Cowboy Wind, LLC, FPL Energy Green Power Wind, LLC, FPL Energy Hancock County Wind, LLC, FPL Energy...

77 FR 27223 - Combined Notice of Filings #1

Federal Register 2010, 2011, 2012, 2013, 2014

2012-05-09

..., LLC, ESI Vansycle Partners, L.P., Florida Power & Light Co., FPL Energy Burleigh County Wind, LLC, FPL Energy Cabazon Wind, LLC, FPL Energy Cape, LLC, FPL Energy Cowboy Wind, LLC, FPL Energy Green Power Wind..., Garden Wind, LLC, Gray County Wind Energy, LLC, Hatch Solar Energy Center I, LLC, Hawkeye Power Partners...

78 FR 32385 - Exelon Generation Company, LLC; CER Generation II, LLC; Constellation Mystic Power, LLC...

Federal Register 2010, 2011, 2012, 2013, 2014

2013-05-30

... DEPARTMENT OF ENERGY Federal Energy Regulatory Commission [Docket No. EL13-64-000] Exelon Generation Company, LLC; CER Generation II, LLC; Constellation Mystic Power, LLC; Constellation NewEnergy...) Rules of Practice and Procedure, 18 CFR 385.207, Exelon Generation Company, LLC, CER Generation II, LLC...

75 FR 62530 - Eagle Creek Hydro Power, LLC; Laredo Ridge Wind, LLC; RRI Energy West, Inc.; Goshen Phase II LLC...

Federal Register 2010, 2011, 2012, 2013, 2014

2010-10-12

...; EG10-52-000; EG10-53-000; EG10- 54-000; EG10-55-000; EG10-56-000] Eagle Creek Hydro Power, LLC; Laredo Ridge Wind, LLC; RRI Energy West, Inc.; Goshen Phase II LLC; Solar Partners I, LLC; Solar Partners II, LLC; Solar Partners VIII, LLC; Notice of Effectiveness of Exempt Wholesale Generator Status October 1...

78 FR 46593 - Prospective Grant of Start-up Exclusive License: Kits for the Detection of Human Interferon-Alpha...

Federal Register 2010, 2011, 2012, 2013, 2014

2013-08-01

... DEPARTMENT OF HEALTH AND HUMAN SERVICES National Institutes of Health Prospective Grant of Start... Services, is contemplating the grant of a start-up exclusive license to practice the inventions embodied in... and Methods of Use'', to IES Diagnostics, LLC having a place of business at 12 Upper Drive, Watchung...

78 FR 31570 - Prospective Grant of a Start-Up Exclusive Patent License Agreement: Treatment of Graves' Disease...

Federal Register 2010, 2011, 2012, 2013, 2014

2013-05-24

... DEPARTMENT OF HEALTH AND HUMAN SERVICES National Institutes of Health Prospective Grant of a Start... of Health and Human Services, is contemplating the grant of a Start-Up Exclusive Patent License to Nova Therapeutics LLC, a company having a place of business in Delaware, to practice the inventions...

PES Holdings, LLC, et. al. Bankruptcy Renewable Fuel Standard Settlement

EPA Pesticide Factsheets

PES Holdings, LLC, North Yard Financing, LLC, North Yard GP, LLC, North Yard Logistics, L.P., PES Administrative Services, LLC, PES Logistics GP, LLC, PES Logistics Partners, L.P., Philadelphia Energy Solutions Refining and Marketing LLC (PESRM),

75 FR 8687 - Combined Notice of Filings #2

Federal Register 2010, 2011, 2012, 2013, 2014

2010-02-25

... Partners LLC, Fenton Power Partners I, LLC, Hoosier Wind Project, LLC, Northwest Wind Partners, LLC, Oasis Power Partners, LLC, Shiloh Wind Project 2, LLC, Wapsipinicon Wind Project, LLC. Description... Service Company of Colorado; Southwestern Public Service Company. Description: Northern States Power...

76 FR 77222 - Combined Notice of Filings #2

Federal Register 2010, 2011, 2012, 2013, 2014

2011-12-12

... Generator Status of Blackwell Wind, LLC under EG12-17. Filed Date: 12/5/11. Accession Number: 20111205-5060... Creek Wind Energy, LLC, Cedar Creek II, LLC, Flat Ridge Wind Energy, LLC, Fowler Ridge II Wind Farm LLC, Fowler Ridge III Wind Farm LLC, Fowler Ridge Wind Farm LLC, Goshen Phase II, LLC, Long Island Solar Farm...

75 FR 4369 - Combined Notice of Filings. #1

Federal Register 2010, 2011, 2012, 2013, 2014

2010-01-27

...; ER04-485-016; ER07-244-010; ER07-245-010; ER07-247-010. Applicants: R.E. Ginna Nuclear Power Plant, LLC..., LLC, Constellation Energy Commodities Group M, Calvert Cliffs Nuclear Power Plant LLC, Raven One, LLC..., Foote Creek IV, LLC, Ridge Crest Wind Partners, LLC, Oak Creek Wind Power, LLC, Foote Creek III, LLC...

77 FR 13318 - SV Hydro, LLC, Coffeeville, LLC, FFP Project 99, LLC; Notice Announcing Filing Priority for...

Federal Register 2010, 2011, 2012, 2013, 2014

2012-03-06

... DEPARTMENT OF ENERGY Federal Energy Regulatory Commission [Project Nos. 14298-000, 14299-000, 14301-000] SV Hydro, LLC, Coffeeville, LLC, FFP Project 99, LLC; Notice Announcing Filing Priority for... follows: 1. FFP Project 99, LLC: Project No. 14301-000. 2. SV Hydro, LLC: Project No. 14298-000. 3...

78 FR 21151 - Boise White Paper, LLC, A Subsidiary of Boise Paper Holdings, LLC, Including On-Site Leased...

Federal Register 2010, 2011, 2012, 2013, 2014

2013-04-09

... Paper, LLC, A Subsidiary of Boise Paper Holdings, LLC, Including On-Site Leased Workers From Guardsmark.... Helens, OR; Boise White Paper, LLC, A Subsidiary of Boise Paper Holdings, LLC, Vancouver, WA; Amended... workers and former workers of Boise White Paper, LLC, a subsidiary of Boise Paper Holdings, LLC, St...

Cancer Diagnosis Epigenomics Scientific Workflow Scheduling in the Cloud Computing Environment Using an Improved PSO Algorithm

PubMed

N, Sadhasivam; R, Balamurugan; M, Pandi

2018-01-27

Objective: Epigenetic modifications involving DNA methylation and histone statud are responsible for the stable maintenance of cellular phenotypes. Abnormalities may be causally involved in cancer development and therefore could have diagnostic potential. The field of epigenomics refers to all epigenetic modifications implicated in control of gene expression, with a focus on better understanding of human biology in both normal and pathological states. Epigenomics scientific workflow is essentially a data processing pipeline to automate the execution of various genome sequencing operations or tasks. Cloud platform is a popular computing platform for deploying large scale epigenomics scientific workflow. Its dynamic environment provides various resources to scientific users on a pay-per-use billing model. Scheduling epigenomics scientific workflow tasks is a complicated problem in cloud platform. We here focused on application of an improved particle swam optimization (IPSO) algorithm for this purpose. Methods: The IPSO algorithm was applied to find suitable resources and allocate epigenomics tasks so that the total cost was minimized for detection of epigenetic abnormalities of potential application for cancer diagnosis. Result: The results showed that IPSO based task to resource mapping reduced total cost by 6.83 percent as compared to the traditional PSO algorithm. Conclusion: The results for various cancer diagnosis tasks showed that IPSO based task to resource mapping can achieve better costs when compared to PSO based mapping for epigenomics scientific application workflow. Creative Commons Attribution License

Improved framework model to allocate optimal rainwater harvesting sites in small watersheds for agro-forestry uses

NASA Astrophysics Data System (ADS)

Terêncio, D. P. S.; Sanches Fernandes, L. F.; Cortes, R. M. V.; Pacheco, F. A. L.

2017-07-01

This study introduces an improved rainwater harvesting (RWH) suitability model to help the implementation of agro-forestry projects (irrigation, wildfire combat) in catchments. The model combines a planning workflow to define suitability of catchments based on physical, socio-economic and ecologic variables, with an allocation workflow to constrain suitable RWH sites as function of project specific features (e.g., distance from rainfall collection to application area). The planning workflow comprises a Multi Criteria Analysis (MCA) implemented on a Geographic Information System (GIS), whereas the allocation workflow is based on a multiple-parameter ranking analysis. When compared to other similar models, improvement comes with the flexible weights of MCA and the entire allocation workflow. The method is tested in a contaminated watershed (the Ave River basin) located in Portugal. The pilot project encompasses the irrigation of a 400 ha crop land that consumes 2.69 Mm3 of water per year. The application of harvested water in the irrigation replaces the use of stream water with excessive anthropogenic nutrients that may raise nitrosamines in the food and accumulation in the food chain, with severe consequences to human health (cancer). The selected rainfall collection catchment is capable to harvest 12 Mm3·yr-1 (≈ 4.5 × the requirement) and is roughly 3 km far from the application area assuring crop irrigation by gravity flow with modest transport costs. The RWH system is an 8-meter high that can be built in earth with reduced costs.

76 FR 40946 - WNC Tax Credits 40, LLC, WNC Tax Credits 41, LLC, WNC Housing Tax Credits Manager 2, LLC, WNC...

Federal Register 2010, 2011, 2012, 2013, 2014

2011-07-12

... Credits 40, LLC, WNC Tax Credits 41, LLC, WNC Housing Tax Credits Manager 2, LLC, WNC National Partners... (``Fund 41'') (each a ``Fund,'' and collectively, the ``Funds''), WNC Housing Tax Credits Manager 2, LLC (the ``Manager''), WNC National Partners, LLC (``WNC National Partners'') and WNC & Associates, Inc...

76 FR 30699 - Combined Notice of Filings #1

Federal Register 2010, 2011, 2012, 2013, 2014

2011-05-26

... DEPARTMENT OF ENERGY Federal Energy Regulatory Commission Combined Notice of Filings 1 Take notice.... Applicants: Evergreen Wind Power, LLC, Canandaigua Power Partners, LLC, Evergreen Wind Power V, LLC, Canandaigua Power Partners II, LLC, Stetson Wind II, LLC, Evergreen Gen Lead, LLC, Vermont Wind, LLC, Niagara...

76 FR 49464 - Combined Notice of Filings #1

Federal Register 2010, 2011, 2012, 2013, 2014

2011-08-10

... Peaker Plant, LLC, California Electric Marketing, LLC, Crete Energy Venture, LLC, High Desert Power..., Bayswater Peaking Facility, LLC, Blythe Energy, LLC, Butler Ridge Wind Energy Center, LLC, Calhoun Power..., ESI Vansycle Partners, L.P., Florida Power & Light Co., FPL Energy Burleigh County Wind, LLC, FPL...

76 FR 2898 - Combined Notice of Filings #1

Federal Register 2010, 2011, 2012, 2013, 2014

2011-01-18

...-002. Applicants: Fenton Power Partners I, LLC, Wapsipinicon Wind Project, LLC, Shiloh Wind Project 2, LLC, Hoosier Wind Project, LLC, Oasis Power Partners, LLC, Chanarambie Power Partners, LLC, Lakefield Wind Project, LLC. Description: Notice of Non-Material Change in Status of Chanarambie Power, et al...

75 FR 7303 - Drake Cement, LLC-Acquisition Exemption-Clarkdale Arizona Central Railroad, LLC

Federal Register 2010, 2011, 2012, 2013, 2014

2010-02-18

... DEPARTMENT OF TRANSPORTATION Surface Transportation Board [STB Finance Docket No. 35350] Drake Cement, LLC--Acquisition Exemption--Clarkdale Arizona Central Railroad, LLC Drake Cement, LLC (DC), a..., Drake Switching Company, LLC --Operation Exemption-- Drake Cement, LLC. DC certifies that the projected...

P185-M Protein Identification and Validation of Results in Workflows that Integrate over Various Instruments, Datasets, Search Engines

PubMed Central

Hufnagel, P.; Glandorf, J.; Körting, G.; Jabs, W.; Schweiger-Hufnagel, U.; Hahner, S.; Lubeck, M.; Suckau, D.

2007-01-01

Analysis of complex proteomes often results in long protein lists, but falls short in measuring the validity of identification and quantification results on a greater number of proteins. Biological and technical replicates are mandatory, as is the combination of the MS data from various workflows (gels, 1D-LC, 2D-LC), instruments (TOF/TOF, trap, qTOF or FTMS), and search engines. We describe a database-driven study that combines two workflows, two mass spectrometers, and four search engines with protein identification following a decoy database strategy. The sample was a tryptically digested lysate (10,000 cells) of a human colorectal cancer cell line. Data from two LC-MALDI-TOF/TOF runs and a 2D-LC-ESI-trap run using capillary and nano-LC columns were submitted to the proteomics software platform ProteinScape. The combined MALDI data and the ESI data were searched using Mascot (Matrix Science), Phenyx (GeneBio), ProteinSolver (Bruker and Protagen), and Sequest (Thermo) against a decoy database generated from IPI-human in order to obtain one protein list across all workflows and search engines at a defined maximum false-positive rate of 5%. ProteinScape combined the data to one LC-MALDI and one LC-ESI dataset. The initial separate searches from the two combined datasets generated eight independent peptide lists. These were compiled into an integrated protein list using the ProteinExtractor algorithm. An initial evaluation of the generated data led to the identification of approximately 1200 proteins. Result integration on a peptide level allowed discrimination of protein isoforms that would not have been possible with a mere combination of protein lists.

77 FR 27041 - Combined Notice of Filings # 1

Federal Register 2010, 2011, 2012, 2013, 2014

2012-05-08

... DEPARTMENT OF ENERGY Federal Energy Regulatory Commission Combined Notice of Filings 1 Take notice...-161-003; ER12-164-002. Applicants: Forward Energy LLC, Sheldon Energy LLC, Invenergy Cannon Falls LLC, Spindle Hill Energy LLC, Spring Canyon Energy LLC, Grays Harbor Energy LLC, Grand Ridge Energy LLC, Willow...

77 FR 68759 - Combined Notice of Filings #2

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2012-11-16

... LLC, BE Rayle LLC, Cedar Brakes I, L.L.C., Cedar Brakes II, L.L.C., Central Power & Lime LLC, Triton Power Michigan LLC, Utility Contract Funding, L.L.C. Description: JPMorgan Sellers' Notice of Non... that the Commission received the following electric rate filings: Docket Numbers: ER10-1821-003...

76 FR 47569 - Combined Notice of Filings #2

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2011-08-05

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76 FR 545 - Combined Notice of Filings #1

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2011-01-05

....; Northern Colorado Wind Energy, LLC; NextEra Energy Power Marketing, LLC; Logan Wind Energy LLC; Peetz Table Wind Energy, LLC; FPL Energy Wyoming, LLC; FPL Energy New Mexico Wind, LLC; FPL Energy Vansycle LLC... DEPARTMENT OF ENERGY Federal Energy Regulatory Commission Combined Notice of Filings 1 December 28...

78 FR 9903 - Combined Notice of Filings #1

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2013-02-12

...-2181-016; ER10-2182-016. Applicants: R.E. Ginna Nuclear Power Plant, LLC, Nine Mile Point Nuclear Station, LLC, Calvert Cliffs Nuclear Power Plant, LLC. Description: Notice of Non-Material Change in... Wind Power, L.L.C., CR Clearing, LLC, Criterion Power Partners, LLC, Exelon Framingham, LLC, Exelon...

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2013-09-13

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78 FR 91 - Combined Notice of Filings #1

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2013-01-02

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Loxoprofen sodium suppresses mouse tumor growth by inhibiting vascular endothelial growth factor.

PubMed

Kanda, Akio; Ebihara, Satoru; Takahashi, Hidenori; Sasaki, Hidetada

2003-01-01

There is increasing evidence to suggest the anti-tumor effects of non-steroidal anti-inflammatory drugs (NSAIDs). In this study it was shown that the most popular NSAID in Japan, loxoprofen sodium (LOX), inhibited in vivo growth of implanted Lewis lung carcinoma (LLC), whereas LOX did not affect the proliferation and viability of LLC cells in vitro. Intratumoral vessel density in LOX-treated mice was significantly lower than that of mice without treatment. Intratumoral expressions of vascular endothelial growth factor (VEGF) mRNA were attenuated by the LOX treatment. LOX suppressed both intratumoral and systemic VEGF protein in LLC-implanted mice. LOX also inhibited tubular formation of primary cultured human umbilical vein endothelial cells, presumably due to the inhibition of VEGF. In patients with advanced non-small cell lung cancer, LOX medication (120 mg/day) for a week significantly decreased the plasma VEGF level. These results suggest that LOX may have potent anti-cancer effects in patients with advanced NSCLC.

Agile parallel bioinformatics workflow management using Pwrake.

PubMed

Mishima, Hiroyuki; Sasaki, Kensaku; Tanaka, Masahiro; Tatebe, Osamu; Yoshiura, Koh-Ichiro

2011-09-08

In bioinformatics projects, scientific workflow systems are widely used to manage computational procedures. Full-featured workflow systems have been proposed to fulfil the demand for workflow management. However, such systems tend to be over-weighted for actual bioinformatics practices. We realize that quick deployment of cutting-edge software implementing advanced algorithms and data formats, and continuous adaptation to changes in computational resources and the environment are often prioritized in scientific workflow management. These features have a greater affinity with the agile software development method through iterative development phases after trial and error.Here, we show the application of a scientific workflow system Pwrake to bioinformatics workflows. Pwrake is a parallel workflow extension of Ruby's standard build tool Rake, the flexibility of which has been demonstrated in the astronomy domain. Therefore, we hypothesize that Pwrake also has advantages in actual bioinformatics workflows. We implemented the Pwrake workflows to process next generation sequencing data using the Genomic Analysis Toolkit (GATK) and Dindel. GATK and Dindel workflows are typical examples of sequential and parallel workflows, respectively. We found that in practice, actual scientific workflow development iterates over two phases, the workflow definition phase and the parameter adjustment phase. We introduced separate workflow definitions to help focus on each of the two developmental phases, as well as helper methods to simplify the descriptions. This approach increased iterative development efficiency. Moreover, we implemented combined workflows to demonstrate modularity of the GATK and Dindel workflows. Pwrake enables agile management of scientific workflows in the bioinformatics domain. The internal domain specific language design built on Ruby gives the flexibility of rakefiles for writing scientific workflows. Furthermore, readability and maintainability of rakefiles may facilitate sharing workflows among the scientific community. Workflows for GATK and Dindel are available at http://github.com/misshie/Workflows.

Agile parallel bioinformatics workflow management using Pwrake

PubMed Central

2011-01-01

Background In bioinformatics projects, scientific workflow systems are widely used to manage computational procedures. Full-featured workflow systems have been proposed to fulfil the demand for workflow management. However, such systems tend to be over-weighted for actual bioinformatics practices. We realize that quick deployment of cutting-edge software implementing advanced algorithms and data formats, and continuous adaptation to changes in computational resources and the environment are often prioritized in scientific workflow management. These features have a greater affinity with the agile software development method through iterative development phases after trial and error. Here, we show the application of a scientific workflow system Pwrake to bioinformatics workflows. Pwrake is a parallel workflow extension of Ruby's standard build tool Rake, the flexibility of which has been demonstrated in the astronomy domain. Therefore, we hypothesize that Pwrake also has advantages in actual bioinformatics workflows. Findings We implemented the Pwrake workflows to process next generation sequencing data using the Genomic Analysis Toolkit (GATK) and Dindel. GATK and Dindel workflows are typical examples of sequential and parallel workflows, respectively. We found that in practice, actual scientific workflow development iterates over two phases, the workflow definition phase and the parameter adjustment phase. We introduced separate workflow definitions to help focus on each of the two developmental phases, as well as helper methods to simplify the descriptions. This approach increased iterative development efficiency. Moreover, we implemented combined workflows to demonstrate modularity of the GATK and Dindel workflows. Conclusions Pwrake enables agile management of scientific workflows in the bioinformatics domain. The internal domain specific language design built on Ruby gives the flexibility of rakefiles for writing scientific workflows. Furthermore, readability and maintainability of rakefiles may facilitate sharing workflows among the scientific community. Workflows for GATK and Dindel are available at http://github.com/misshie/Workflows. PMID:21899774

76 FR 81926 - Notice of Effectiveness of Exempt Wholesale Generator Status

Federal Register 2010, 2011, 2012, 2013, 2014

2011-12-29

... Renewables, LLC EG11-126-000 Invenergy Illinois Solar I LLC EG11-127-000 Record Hill Wind LLC EG11-128-000 Vasco Winds, LLC EG11-129-000 NexEra Energy Montezuma II Wind, LLC EG11-130-000 Richard-Stryker Generation LLC EG11-131-000 Pioneer Trail Wind Farm, LLC EG11-132-000 GSG 6, LLC EG11-133-000 Take notice...

75 FR 76453 - Top of the World Wind Energy, LLC; Kit Carson Windpower, LLC; Chestnut Flats Wind, LLC; Minco...

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2010-12-08

... DEPARTMENT OF ENERGY Federal Energy Regulatory Commission [Docket Nos. EG10-65-000; EG10-66-000; EG10-67-000; EG10-68-000; EG10- 69-000; EG10-70-000; EG10-71-000] Top of the World Wind Energy, LLC; Kit Carson Windpower, LLC; Chestnut Flats Wind, LLC; Minco Wind, LLC; Arizona Solar One LLC; Criterion...

77 FR 48135 - Combined Notice of Filings #1

Federal Register 2010, 2011, 2012, 2013, 2014

2012-08-13

...: J.P. Morgan Ventures Energy Corporation, Triton Power Michigan LLC, BE Allegheny LLC, BE CA LLC, BE... Contract Funding, L.L.C., Central Power & Lime LLC, Cedar Brakes II, L.L.C., J.P. Morgan Commodities Canada...; ER10-2898-008. Applicants: J.P. Morgan Ventures Energy Corporation, Triton Power Michigan LLC, BE...

78 FR 40735 - Combined Notice of Filings #2

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2013-07-08

...: Iberdrola Renewables, LLC, Atlantic Renewable Projects II LLC, Dillon Wind LLC, Dry Lake Wind Power, LLC, Dry Lake Wind Power II LLC, Manzana Wind LLC, Mountain View Power Partners III, LLC, Shiloh I Wind... Market Power Analysis in the Northwest Region of Puget Sound Energy, Inc., et. al. Filed Date: 6/28/13...

76 FR 19473 - Chrysler Financial Services Americas, LLC, a Subsidiary of FinCo Intermediate Holding Co., LLC...

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2011-04-07

... Services Americas, LLC, a Subsidiary of FinCo Intermediate Holding Co., LLC, Troy Customer Contact Center... Co., LLC, Troy Customer Contact Center, Troy, Michigan (subject firm). The Department's Notice was... Services Americas, LLC, a subsidiary of FinCo Intermediate Holding Co., LLC, Troy Customer Contact Center...

Optimizing Human Cognition

DTIC Science & Technology

2013-01-01

decision, unless so designated by other documentation. 12. DISTRIBUTION AVAILIBILITY STATEMENT Approved for public release; distribution is unlimited. UU 9...SPONSORING/MONITORING AGENCY NAME(S) AND ADDRESS(ES) 6. AUTHORS 7. PERFORMING ORGANIZATION NAMES AND ADDRESSES U.S. Army Research Office P.O. Box...12211 Research Triangle Park, NC 27709-2211 15. SUBJECT TERMS Human Attention Eran Zaidel Pacific Development and Technology LLC 999 Commercial St

N-domain angiotensin-I converting enzyme is expressed in immortalized mesangial, proximal tubule and collecting duct cells.

PubMed

Mei Wang, Pamella Huey; Andrade, Maria Claudina; Quinto, Beata Marie Redublo; Di Marco, Giovana; Mortara, Renato Arruda; Vio, Carlos P; Casarini, Dulce Elena

2015-01-01

Somatic ACE (sACE) is found in glomerulus, proximal tubule and excreted in urine. We hypothesized that N-domain ACE can also be found at these sites. ACE profile was analyzed in mesangial (IMC), proximal (LLC-PK1), distal tubule (MDCK) and collecting duct (IMCD) cells. Cell lysate and culture medium were submitted to gel filtration chromatography, which separated two peaks with ACE activity from cells and medium, except from distal tubule. The first had a high molecular weight and the second, a lower one (65 kDa; N-domain ACE). We focused on N-domain ACE purification and characterization from LLC-PK1. Total LLC-PK1 N-domain ACE purification was achieved by ion-exchange chromatography, which presented only one peak with ACE activity, denominated ACE(int2A). ACE(int2A) activity was influenced by pH, NaCl and temperature. The purified enzyme was inhibited by Captopril and hydrolyzed AngI, Ang1-7 and AcSDKP. Its ability to hydrolyze AcSDKP characterized it as an N-domain ACE. ACE(int2A) also presented high amino acid sequence homology with the N-terminal part of sACE from mouse, rat, human and rabbit. The presence of secreted and intracellular N-domain ACE and sACE in IMC, LLC-PK1 and IMCD cells confirmed our studies along the nephron. We identified, purified and characterized N-domain ACE from LLC-PK1. Copyright © 2014 Elsevier B.V. All rights reserved.

76 FR 41771 - Combined Notice of Filings #1

Federal Register 2010, 2011, 2012, 2013, 2014

2011-07-15

..., LLC, Ashtabula Wind III, LLC, Badger Windpower, LLC, Butler Ridge Wind Energy Center, LLC, Crystal Lake Wind, LLC, Crystal Lake Winder II, LLC, Crystal Lake Wind III, LLC, FPL Energy Hancock County Wind.... Eastern Time on Friday, July 29, 2011. Docket Numbers: ER10-2670-003; ER10-2669-003; ER10-2671-003; ER10...

76 FR 74779 - SV Hydro LLC; Coffeeville LLC; FFP Project 99 LLC; Lock Hydro Friends Fund XIV; Notice of...

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2011-12-01

... DEPARTMENT OF ENERGY Federal Energy Regulatory Commission [Project Nos. 14298-000; 14299-000; 14301-000; 14302-000] SV Hydro LLC; Coffeeville LLC; FFP Project 99 LLC; Lock Hydro Friends Fund XIV... (Coffeeville), FFP Project 99 LLC (FFP 99), and Lock Hydro Friends Fund XIV (Lock Hydro) filed preliminary...

76 FR 13613 - Supplemental Notice of Technical Conference

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2011-03-14

... Officer, Tonbridge Power, Inc. [rtarr8] Tyson Utt, Project Manager, Horizon Wind Energy LLC [rtarr8... Conference Docket No. Priority Rights to New Participant- AD11-11-000 Funded Transmission. Alta Wind I, LLC EL10-62-000 Alta Wind II, LLC Alta Wind III, LLC Alta Wind IV, LLC Alta Wind V, LLC Alta Wind VI, LLC...

77 FR 10739 - SV Hydro, LLC, Coffeeville, LLC, FFP Project 99, LLC; Notice Announcing Preliminary Permit Drawing

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2012-02-23

... DEPARTMENT OF ENERGY Federal Energy Regulatory Commission [Project No. 14298-000, Project No. 14299-000, Project No. 14301-000] SV Hydro, LLC, Coffeeville, LLC, FFP Project 99, LLC; Notice... Project No. 14299-000, and FFP Project 99, LLC for Project No. 14301-000.\\2\\ \\1\\ Under the Commission's...

78 FR 22538 - Combined Notice of Filings #1

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2013-04-16

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76 FR 27041 - Combined Notice of Filings #1

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2011-05-10

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It's All About the Data: Workflow Systems and Weather

NASA Astrophysics Data System (ADS)

Plale, B.

2009-05-01

Digital data is fueling new advances in the computational sciences, particularly geospatial research as environmental sensing grows more practical through reduced technology costs, broader network coverage, and better instruments. e-Science research (i.e., cyberinfrastructure research) has responded to data intensive computing with tools, systems, and frameworks that support computationally oriented activities such as modeling, analysis, and data mining. Workflow systems support execution of sequences of tasks on behalf of a scientist. These systems, such as Taverna, Apache ODE, and Kepler, when built as part of a larger cyberinfrastructure framework, give the scientist tools to construct task graphs of execution sequences, often through a visual interface for connecting task boxes together with arcs representing control flow or data flow. Unlike business processing workflows, scientific workflows expose a high degree of detail and control during configuration and execution. Data-driven science imposes unique needs on workflow frameworks. Our research is focused on two issues. The first is the support for workflow-driven analysis over all kinds of data sets, including real time streaming data and locally owned and hosted data. The second is the essential role metadata/provenance collection plays in data driven science, for discovery, determining quality, for science reproducibility, and for long-term preservation. The research has been conducted over the last 6 years in the context of cyberinfrastructure for mesoscale weather research carried out as part of the Linked Environments for Atmospheric Discovery (LEAD) project. LEAD has pioneered new approaches for integrating complex weather data, assimilation, modeling, mining, and cyberinfrastructure systems. Workflow systems have the potential to generate huge volumes of data. Without some form of automated metadata capture, either metadata description becomes largely a manual task that is difficult if not impossible under high-volume conditions, or the searchability and manageability of the resulting data products is disappointingly low. The provenance of a data product is a record of its lineage, or trace of the execution history that resulted in the product. The provenance of a forecast model result, e.g., captures information about the executable version of the model, configuration parameters, input data products, execution environment, and owner. Provenance enables data to be properly attributed and captures critical parameters about the model run so the quality of the result can be ascertained. Proper provenance is essential to providing reproducible scientific computing results. Workflow languages used in science discovery are complete programming languages, and in theory can support any logic expressible by a programming language. The execution environments supporting the workflow engines, on the other hand, are subject to constraints on physical resources, and hence in practice the workflow task graphs used in science utilize relatively few of the cataloged workflow patterns. It is important to note that these workflows are executed on demand, and are executed once. Into this context is introduced the need for science discovery that is responsive to real time information. If we can use simple programming models and abstractions to make scientific discovery involving real-time data accessible to specialists who share and utilize data across scientific domains, we bring science one step closer to solving the largest of human problems.

Flexible Early Warning Systems with Workflows and Decision Tables

NASA Astrophysics Data System (ADS)

Riedel, F.; Chaves, F.; Zeiner, H.

2012-04-01

An essential part of early warning systems and systems for crisis management are decision support systems that facilitate communication and collaboration. Often official policies specify how different organizations collaborate and what information is communicated to whom. For early warning systems it is crucial that information is exchanged dynamically in a timely manner and all participants get exactly the information they need to fulfil their role in the crisis management process. Information technology obviously lends itself to automate parts of the process. We have experienced however that in current operational systems the information logistics processes are hard-coded, even though they are subject to change. In addition, systems are tailored to the policies and requirements of a certain organization and changes can require major software refactoring. We seek to develop a system that can be deployed and adapted to multiple organizations with different dynamic runtime policies. A major requirement for such a system is that changes can be applied locally without affecting larger parts of the system. In addition to the flexibility regarding changes in policies and processes, the system needs to be able to evolve; when new information sources become available, it should be possible to integrate and use these in the decision process. In general, this kind of flexibility comes with a significant increase in complexity. This implies that only IT professionals can maintain a system that can be reconfigured and adapted; end-users are unable to utilise the provided flexibility. In the business world similar problems arise and previous work suggested using business process management systems (BPMS) or workflow management systems (WfMS) to guide and automate early warning processes or crisis management plans. However, the usability and flexibility of current WfMS are limited, because current notations and user interfaces are still not suitable for end-users, and workflows are usually only suited for rigid processes. We show how improvements can be achieved by using decision tables and rule-based adaptive workflows. Decision tables have been shown to be an intuitive tool that can be used by domain experts to express rule sets that can be interpreted automatically at runtime. Adaptive workflows use a rule-based approach to increase the flexibility of workflows by providing mechanisms to adapt workflows based on context changes, human intervention and availability of services. The combination of workflows, decision tables and rule-based adaption creates a framework that opens up new possibilities for flexible and adaptable workflows, especially, for use in early warning and crisis management systems.

MetaboTools: A comprehensive toolbox for analysis of genome-scale metabolic models

DOE PAGES

Aurich, Maike K.; Fleming, Ronan M. T.; Thiele, Ines

2016-08-03

Metabolomic data sets provide a direct read-out of cellular phenotypes and are increasingly generated to study biological questions. Previous work, by us and others, revealed the potential of analyzing extracellular metabolomic data in the context of the metabolic model using constraint-based modeling. With the MetaboTools, we make our methods available to the broader scientific community. The MetaboTools consist of a protocol, a toolbox, and tutorials of two use cases. The protocol describes, in a step-wise manner, the workflow of data integration, and computational analysis. The MetaboTools comprise the Matlab code required to complete the workflow described in the protocol. Tutorialsmore » explain the computational steps for integration of two different data sets and demonstrate a comprehensive set of methods for the computational analysis of metabolic models and stratification thereof into different phenotypes. The presented workflow supports integrative analysis of multiple omics data sets. Importantly, all analysis tools can be applied to metabolic models without performing the entire workflow. Taken together, the MetaboTools constitute a comprehensive guide to the intra-model analysis of extracellular metabolomic data from microbial, plant, or human cells. In conclusion, this computational modeling resource offers a broad set of computational analysis tools for a wide biomedical and non-biomedical research community.« less

A Comprehensive Workflow of Mass Spectrometry-Based Untargeted Metabolomics in Cancer Metabolic Biomarker Discovery Using Human Plasma and Urine

PubMed Central

Zou, Wei; She, Jianwen; Tolstikov, Vladimir V.

2013-01-01

Current available biomarkers lack sensitivity and/or specificity for early detection of cancer. To address this challenge, a robust and complete workflow for metabolic profiling and data mining is described in details. Three independent and complementary analytical techniques for metabolic profiling are applied: hydrophilic interaction liquid chromatography (HILIC–LC), reversed-phase liquid chromatography (RP–LC), and gas chromatography (GC). All three techniques are coupled to a mass spectrometer (MS) in the full scan acquisition mode, and both unsupervised and supervised methods are used for data mining. The univariate and multivariate feature selection are used to determine subsets of potentially discriminative predictors. These predictors are further identified by obtaining accurate masses and isotopic ratios using selected ion monitoring (SIM) and data-dependent MS/MS and/or accurate mass MSn ion tree scans utilizing high resolution MS. A list combining all of the identified potential biomarkers generated from different platforms and algorithms is used for pathway analysis. Such a workflow combining comprehensive metabolic profiling and advanced data mining techniques may provide a powerful approach for metabolic pathway analysis and biomarker discovery in cancer research. Two case studies with previous published data are adapted and included in the context to elucidate the application of the workflow. PMID:24958150

Using WorldView-2 Imagery to Track Flooding in Thailand in a Multi-Asset Sensorweb

NASA Technical Reports Server (NTRS)

McLaren, David; Doubleday, Joshua; Chien, Steve

2012-01-01

For the flooding seasons of 2011-2012 multiple space assets were used in a "sensorweb" to track major flooding in Thailand. Worldview-2 multispectral data was used in this effort and provided extremely high spatial resolution (2m / pixel) multispectral (8 bands at 0.45-1.05 micrometer spectra) data from which mostly automated workflows derived surface water extent and volumetric water information for use by a range of NGO and national authorities. We first describe how Worldview-2 and its data was integrated into the overall flood tracking sensorweb. We next describe the use of Support Vector Machine learning techniques that were used to derive surface water extent classifiers. Then we describe the fusion of surface water extent and digital elevation map (DEM) data to derive volumetric water calculations. Finally we discuss key future work such as speeding up the workflows and automating the data registration process (the only portion of the workflow requiring human input).

Improving Clinical Workflow in Ambulatory Care: Implemented Recommendations in an Innovation Prototype for the Veteran’s Health Administration

PubMed Central

Patterson, Emily S.; Lowry, Svetlana Z.; Ramaiah, Mala; Gibbons, Michael C.; Brick, David; Calco, Robert; Matton, Greg; Miller, Anne; Makar, Ellen; Ferrer, Jorge A.

2015-01-01

Introduction: Human factors workflow analyses in healthcare settings prior to technology implemented are recommended to improve workflow in ambulatory care settings. In this paper we describe how insights from a workflow analysis conducted by NIST were implemented in a software prototype developed for a Veteran’s Health Administration (VHA) VAi2 innovation project and associated lessons learned. Methods: We organize the original recommendations and associated stages and steps visualized in process maps from NIST and the VA’s lessons learned from implementing the recommendations in the VAi2 prototype according to four stages: 1) before the patient visit, 2) during the visit, 3) discharge, and 4) visit documentation. NIST recommendations to improve workflow in ambulatory care (outpatient) settings and process map representations were based on reflective statements collected during one-hour discussions with three physicians. The development of the VAi2 prototype was conducted initially independently from the NIST recommendations, but at a midpoint in the process development, all of the implementation elements were compared with the NIST recommendations and lessons learned were documented. Findings: Story-based displays and templates with default preliminary order sets were used to support scheduling, time-critical notifications, drafting medication orders, and supporting a diagnosis-based workflow. These templates enabled customization to the level of diagnostic uncertainty. Functionality was designed to support cooperative work across interdisciplinary team members, including shared documentation sessions with tracking of text modifications, medication lists, and patient education features. Displays were customized to the role and included access for consultants and site-defined educator teams. Discussion: Workflow, usability, and patient safety can be enhanced through clinician-centered design of electronic health records. The lessons learned from implementing NIST recommendations to improve workflow in ambulatory care using an EHR provide a first step in moving from a billing-centered perspective on how to maintain accurate, comprehensive, and up-to-date information about a group of patients to a clinician-centered perspective. These recommendations point the way towards a “patient visit management system,” which incorporates broader notions of supporting workload management, supporting flexible flow of patients and tasks, enabling accountable distributed work across members of the clinical team, and supporting dynamic tracking of steps in tasks that have longer time distributions. PMID:26290887

Innovatively Therapeutic Strategy on Lung Cancer by Daily Drinking Antioxidative Plasmon-Induced Activated Water.

PubMed

Wang, Chien-Kai; Chen, Hsiao-Chien; Fang, Sheng-Uei; Ho, Chia-Wen; Tai, Cheng-Jeng; Yang, Chih-Ping; Liu, Yu-Chuan

2018-04-20

Many human diseases are inflammation-related, such as cancer and those associated with aging. Previous studies demonstrated that plasmon-induced activated (PIA) water with electron-doping character, created from hot electron transfer via decay of excited Au nanoparticles (NPs) under resonant illumination, owns reduced hydrogen-bonded networks and physchemically antioxidative properties. In this study, it is demonstrated PIA water dramatically induced a major antioxidative Nrf2 gene in human gingival fibroblasts which further confirms its cellular antioxidative and anti-inflammatory properties. Furthermore, mice implanted with mouse Lewis lung carcinoma (LLC-1) cells drinking PIA water alone or together with cisplatin treatment showed improved survival time compared to mice which consumed only deionized (DI) water. With the combination of PIA water and cisplatin administration, the survival time of LLC-1-implanted mice markedly increased to 8.01 ± 0.77 days compared to 6.38 ± 0.61 days of mice given cisplatin and normal drinking DI water. This survival time of 8.01 ± 0.77 days compared to 4.62 ± 0.71 days of mice just given normal drinking water is statistically significant (p = 0.009). Also, the gross observations and eosin staining results suggested that LLC-1-implanted mice drinking PIA water tended to exhibit less metastasis than mice given only DI water.

78 FR 34092 - Lock+ Hydro Friends Fund XXX, LLC; FFP Project 121, LLC; Notice of Competing Preliminary Permit...

Federal Register 2010, 2011, 2012, 2013, 2014

2013-06-06

.... 14504-000] Lock+ Hydro Friends Fund XXX, LLC; FFP Project 121, LLC; Notice of Competing Preliminary... Applications Lock+ Hydro Friends Fund XXX, LLC and FFP Project 121, LLC filed preliminary permit applications... regular business day. See id. at 385.2001(a)(2). Lock+ Hydro Friends Fund XXX, LLC's application is for a...

75 FR 55315 - National Fuel Marketing Company, LLC; NFM Midstream, LLC; NFM Texas Pipeline, LLC; NFM Texas...

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2010-09-10

... DEPARTMENT OF ENERGY Federal Energy Regulatory Commission [Docket No. IN09-10-000] National Fuel Marketing Company, LLC; NFM Midstream, LLC; NFM Texas Pipeline, LLC; NFM Texas Gathering, LLC; Notice of Amended Designation of Commission Staff as Non-Decisional September 2, 2010. On January 15, 2009, the Commission issued an order in the above-...

75 FR 17976 - WNC Tax Credits 38, LLC, WNC Tax Credits 39, LLC, WNC Housing Tax Credits Manager, LLC and WNC...

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2010-04-08

... Credits 38, LLC, WNC Tax Credits 39, LLC, WNC Housing Tax Credits Manager, LLC and WNC & Associates, Inc... collectively, the ``Funds''), WNC Housing Tax Credits Manager, LLC (the ``Manager'') and WNC & Associates, Inc... credit under the Internal Revenue Code of 1986, as amended. The Manager is a California limited liability...

76 FR 58260 - FPL Energy Maine Hydro LLC; Madison Paper Industries; Merimil Limited Partnership; Notice of...

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2011-09-20

... (FEA) for the project. The FEA contains staff's analysis of the potential environmental effects of... human environment. A copy of the FEA is available for review at the Commission in the Public [[Page...

75 FR 5312 - ETC Tiger Pipeline Company, LLC; Notice of Availability of the Environmental Assessment for the...

Federal Register 2010, 2011, 2012, 2013, 2014

2010-02-02

... quality of the human environment. This EA was prepared in cooperation with U.S. Army Corps of Engineers, U... Three pig launcher/receiver facilities associated with the Carthage and Bienville Compressor Stations...

78 FR 41791 - Combined Notice of Filings #2

Federal Register 2010, 2011, 2012, 2013, 2014

2013-07-11

... DEPARTMENT OF ENERGY Federal Energy Regulatory Commission Combined Notice of Filings 2 Take notice.... Applicants: Sundevil Power Holdings, LLC, Castleton Energy Services, LLC, Castleton Power, LLC. Description...-1971-011. Applicants: Diablo Winds, LLC, FPL Energy Cabazon Wind, LLC, FPL Energy Green Power Wind, LLC...

75 FR 71112 - Combined Notice of Filings #1

Federal Register 2010, 2011, 2012, 2013, 2014

2010-11-22

...: ArcLight Energy Marketing, LLC, Oak Creek Wind Power, LLC, Coso Geothermal Power Holdings, LLC.... Applicants: ArcLight Energy Marketing, LLC, Oak Creek Wind Power, LLC, Coso Geothermal Power Holdings, LLC... DEPARTMENT OF ENERGY Federal Energy Regulatory Commission Combined Notice of Filings 1 November 15...

76 FR 72402 - Combined Notice of Filings #1

Federal Register 2010, 2011, 2012, 2013, 2014

2011-11-23

...-772-003. Applicants: ExxonMobil Baton Rouge Complex. Description: ExxonMobil Entities submits their... Bethlehem, LLC, Calpine Mid-Atlantic Generation, LLC, Calpine Mid Merit, LLC, Calpine New Jersey Generation... City Generation, L.P., Forward WindPower, LLC, Lookout WindPower, LLC, Midwest Generation, LLC...

75 FR 2531 - Combined Notice of Filings #1

Federal Register 2010, 2011, 2012, 2013, 2014

2010-01-15

... Power, LLC, Terra-Gen VG Wind, LLC, Terra-Gen 251 Wind, LLC, Chandler Wind Partners, LLC. Description... Power Source Generation, Inc., Calvert Cliffs Nuclear Power Plant LLC, Constellation Energy Commodities..., Inc., Constellation Energy Commodities Group Maine, LLC, R.E. Ginna Nuclear Power Plant, Raven One...

76 FR 65716 - Notice of Effectiveness of Exempt Wholesale Generator Status

Federal Register 2010, 2011, 2012, 2013, 2014

2011-10-24

... South, LLC EG11-104-000 Gratiot County Wind LLC EG11-105-000 Invenergy Wind Development Michigan LLC EG11-106-000 Trinity Hills Wind Farm LLC EG11-107-000 High Plains Ranch II, LLC EG11-108-000 Double ``C... Wholesale Generator Status Docket No. Michigan Wind 2, LLC EG11-100-000 Bishop Hill Energy LLC EG11-101-000...

77 FR 60981 - TGP Granada, LLC and Roosevelt Wind Ranch, LLC v. Public Service Company of New Mexico, Tortoise...

Federal Register 2010, 2011, 2012, 2013, 2014

2012-10-05

...; EL12-43-000, EL12-43-001 TGP Granada, LLC and Roosevelt Wind Ranch, LLC v. Public Service Company of New Mexico, Tortoise Capital Resources Corp.; TGP Granada, LLC and Roosevelt Wind Ranch, LLC; Notice... over capacity on the Eastern Interconnection Project. \\1\\ TGP Granada, LLC v. Pub. Serv. Co. of New...

75 FR 12230 - Black Oak Energy, L.L.C., EPIC Merchant Energy, LP, SESCO Enterprises, LLC v. PJM Interconnection...

Federal Register 2010, 2011, 2012, 2013, 2014

2010-03-15

... Energy, L.L.C., EPIC Merchant Energy, LP, SESCO Enterprises, LLC v. PJM Interconnection, L.L.C.; Notice of Filing March 8, 2010. Take notice that on March 1, 2010, PJM Interconnection, L.L.C. filed a..., Order Accepting Compliance filing issued in this proceeding, Black Oak Energy, L.L.C., et al. v. PJM...

A Bioinformatics Workflow for Variant Peptide Detection in Shotgun Proteomics*

PubMed Central

Li, Jing; Su, Zengliu; Ma, Ze-Qiang; Slebos, Robbert J. C.; Halvey, Patrick; Tabb, David L.; Liebler, Daniel C.; Pao, William; Zhang, Bing

2011-01-01

Shotgun proteomics data analysis usually relies on database search. However, commonly used protein sequence databases do not contain information on protein variants and thus prevent variant peptides and proteins from been identified. Including known coding variations into protein sequence databases could help alleviate this problem. Based on our recently published human Cancer Proteome Variation Database, we have created a protein sequence database that comprehensively annotates thousands of cancer-related coding variants collected in the Cancer Proteome Variation Database as well as noncancer-specific ones from the Single Nucleotide Polymorphism Database (dbSNP). Using this database, we then developed a data analysis workflow for variant peptide identification in shotgun proteomics. The high risk of false positive variant identifications was addressed by a modified false discovery rate estimation method. Analysis of colorectal cancer cell lines SW480, RKO, and HCT-116 revealed a total of 81 peptides that contain either noncancer-specific or cancer-related variations. Twenty-three out of 26 variants randomly selected from the 81 were confirmed by genomic sequencing. We further applied the workflow on data sets from three individual colorectal tumor specimens. A total of 204 distinct variant peptides were detected, and five carried known cancer-related mutations. Each individual showed a specific pattern of cancer-related mutations, suggesting potential use of this type of information for personalized medicine. Compatibility of the workflow has been tested with four popular database search engines including Sequest, Mascot, X!Tandem, and MyriMatch. In summary, we have developed a workflow that effectively uses existing genomic data to enable variant peptide detection in proteomics. PMID:21389108

76 FR 11238 - Notice of Technical Conference

Federal Register 2010, 2011, 2012, 2013, 2014

2011-03-01

... DEPARTMENT OF ENERGY Federal Energy Regulatory Commission Notice of Technical Conference Docket No... Alta Wind VIII, LLC Alta Windpower Development, LLC TGP Development Company, LLC Puget Sound Energy... York Canyon, LLC Green Borders Geothermal, LLC v. Terra-Gen Dixie EL10-36-002 Valley, LLC Terra-Gen...

78 FR 67354 - Combined Notice of Filings #1

Federal Register 2010, 2011, 2012, 2013, 2014

2013-11-12

.... Description: Simon Solar, LLC submits Supplement Record in Pending Filing to be effective 10/1/2013. Filed.... Applicants: All Dams Generation, LLC, Arlington Valley Solar Energy II, LLC, Bluegrass Generation Company, L.L.C., Calhoun Power Company, LLC, Centinela Solar Energy, LLC, Cherokee County Cogeneration Partners...

78 FR 42060 - Combined Notice of Filings #2

Federal Register 2010, 2011, 2012, 2013, 2014

2013-07-15

... Harbor Water Power Corporation, PECO Energy Company, Michigan Wind 1, LLC, Michigan Wind 2, LLC, Harvest... Clearing, LLC, Cow Branch Wind Power, L.L.C., Constellation Power Source Generation Inc., Constellation New..., Calvert Cliffs Nuclear Power Plant, LLC, Nine Mine Point Nuclear Station, LLC. Description: Revised...

75 FR 63457 - Combined Notice of Filings #1

Federal Register 2010, 2011, 2012, 2013, 2014

2010-10-15

... Company, Wind Capital Holdings, LLC, CR Clearing, LLC, Cow Branch Wind Power LLC, JD WIND 4, LLC, Harvest... Power Marketing, LP, Exelon Energy Company, Cassia Gulch Wind Park, Michigan Wind 1, LLC, Tuana Springs...-000. Applicants: Ashtabula Wind III, LLC. Description: Notice of Self-Certification of Exempt...

75 FR 13531 - Combined Notice of Filings #1

Federal Register 2010, 2011, 2012, 2013, 2014

2010-03-22

... Partners, LLC, Lake Benton Power Partners LLC, Langdon Wind, LLC, Osceola Windpower, LLC, Osceola Windpower... Company submits the non-conforming service agreement under cost-based power sales tariff with The Energy... Nuclear Power Marketing, LLC, Entergy Nuclear Vermont Yankee, LLC, Entergy Power Ventures, L.P., Entergy...

76 FR 17120 - Supplemental Notice Requesting Comments

Federal Register 2010, 2011, 2012, 2013, 2014

2011-03-28

... Wind VII, LLC Alta Wind VIII, LLC Alta Windpower Development, LLC TGP Development Company, LLC Puget... Creek Wind Energy, LLC RC11-1-000 Milford Wind Corridor Phase I, LLC........ RC11-2-000 SunZia... DEPARTMENT OF ENERGY Federal Energy Regulatory Commission Supplemental Notice Requesting Comments...

Study on workloads of human care worker with the introduction of IT system - the characteristics of work loads by observational research and the suggestions for KAIZEN.

PubMed

Mizuno, Yuki; Yoshikawa, Toru; Matsuda, Fumiko; Takeuchi, Yuriko; Motegi, Nobuyuki; Ikegami, Thor; Sakai, Kazuhiro

2012-01-01

The purpose of this study was to clarify the characteristic of workloads on human care worker with the introduction of IT system, and suggested the support measures for KAIZEN in Japan. The investigation method is workflow line and hearing with a focus on work observation. The objects were 8 human care workers of the acute hospital that introduced an electronic system. By the introduction of the electronic chart, the nurse station sojourn time decreased, sickroom sojourn time increased, and time about direct nursing care to a patient increased. In addition, access to patient information became easy, and the offer of the health care service based on correct information came to be possible in real time. By The point of workflow line, it was effect that moving lengths decreased in order to install the electronic chart in patients' rooms. Though, it was a problem that it hasn't formed where to place the instruments such as sphygmomanometer, clinical thermometer and others.

75 FR 6197 - Combined Notice of Filings #1

Federal Register 2010, 2011, 2012, 2013, 2014

2010-02-08

... Contract Funding, L.L.C., Vineland Energy LLC, Central Power & Lime LLC, Cedar Brakes II, L.L.C., J.P..., 2010. Take notice that the Commission received the following electric rate filings: Docket Numbers: ER97-870-016. Applicants: Sunoco Power Marketing LLC. Description: Sunoco Power Marketing, LLC submits...

78 FR 4142 - Combined Notice of Filings #1

Federal Register 2010, 2011, 2012, 2013, 2014

2013-01-18

... I, L.L.C., Cedar Brakes II, L.L.C., Central Power & Lime LLC, Utility Contract Funding, L.L.C... Energy Services, LLC, Champion Energy, LLC. Description: Updated Market Power Analysis for the Southwest... that the Commission received the following electric rate filings: Docket Numbers: ER10-2331-014; ER10...

78 FR 15053 - Simpson Lumber Company, LLC, Shelton, Washington; Simpson Lumber Company, LLC, Tacoma, Washington...

Federal Register 2010, 2011, 2012, 2013, 2014

2013-03-08

...,372B] Simpson Lumber Company, LLC, Shelton, Washington; Simpson Lumber Company, LLC, Tacoma, Washington; Simpson Lumber Company, LLC, Longview, Washington; Notice of Revised Determination on Reconsideration On... Reconsideration for the workers and former workers of Simpson Lumber Company, LLC, Shelton, Washington (TA-W-81...

75 FR 30391 - Combined Notice of Filings #1

Federal Register 2010, 2011, 2012, 2013, 2014

2010-06-01

... Time on Tuesday, June 8, 2010. Docket Numbers: EC10-72-000. Applicants: Cloud County Wind Farm, LLC, Pioneer Prairie Wind Farm I, LLC, Arlington Wind Power Project LLC. Description: Application for... Power Project LLC, Cloud County Wind Farm, LLC, and Pioneer Prairie Wind Farm I, LLC. Filed Date: 05/20...

76 FR 4100 - Combined Notice of Filings #1

Federal Register 2010, 2011, 2012, 2013, 2014

2011-01-24

..., Rolling Thunder I Power Partners, LLC, Goshen Phase II LLC, Cedar Creek II, LLC. Description: Supplemental Information of Rolling Thunder I Power Partners, LLC. Filed Date: 01/12/2011. Accession Number: 20110112-5154...: Rolling Thunder I Power Partners, LLC. Description: Rolling Thunder I Power Partners, LLC submits tariff...

77 FR 38884 - Professional Transportation, Inc.-Asset Acquisition-CUSA ES, LLC and CUSA CSS, LLC

Federal Register 2010, 2011, 2012, 2013, 2014

2012-06-29

..., Inc.--CUSA ES, LLC (MC-463168) and CUSA CSS, LLC (MC-522544) (collectively, Coach America Subsidiaries... Transportation, Inc.--Asset Acquisition--CUSA ES, LLC and CUSA CSS, LLC AGENCY: Surface Transportation Board. ACTION: Notice of Finance Application. SUMMARY: Professional Transportation, Inc. (PTI or Applicant), an...

76 FR 1416 - Combined Notice of Filings #1

Federal Register 2010, 2011, 2012, 2013, 2014

2011-01-10

... Wind Farm LLC; State Line Energy, LLC; Kincaid Generation, L.L.C.; Virginia Electric and Power Company...: Constellation Energy Commodities Group, R.E. Ginna Nuclear Power Plant, LLC, AES NewEnergy, Inc., Baltimore Gas..., Safe Harbor Water Power Corporation, Calvert Cliffs Nuclear Power Plant LLC, CER Generation, LLC...

78 FR 38705 - Combined Notice of Filings #1

Federal Register 2010, 2011, 2012, 2013, 2014

2013-06-27

.... Applicants: Copper Mountain Solar 1, LLC, Copper Mountain Solar 2, LLC, Energia Sierra Juarez U.S., LLC, Mesquite Power, LLC, Mesquite Solar 1, LLC, San Diego Gas & Electric Company, Sempra Generation... Analysis for the Southwest Region of Copper Mountain Solar 1, LLC, et al. Filed Date: 6/19/13. Accession...

77 FR 75701 - Landisville Railroad, LLC-Operation Exemption-Buckeye East Chicago Railroad LLC

Federal Register 2010, 2011, 2012, 2013, 2014

2012-12-21

... Railroad, LLC--Operation Exemption--Buckeye East Chicago Railroad LLC Landisville Railroad, LLC... operate approximately 7,065 feet (1.34 miles) of track,\\1\\ existing railroad right-of-way, and bulk liquid transloading facilities owned by Buckeye East Chicago Railroad, LLC, a Class III rail carrier, in East Chicago...

75 FR 76520 - Tennessee Southern Railroad Company, Patriot Rail, LLC, Patriot Rail Holdings LLC, and Patriot...

Federal Register 2010, 2011, 2012, 2013, 2014

2010-12-08

... family by converting two of its subsidiaries from corporations into limited liability companies: (1... Railroad Company, Patriot Rail, LLC, Patriot Rail Holdings LLC, and Patriot Rail Corp.--Corporate Family... Railroad Company (TSRR), Patriot Rail, LLC (PRL) and its subsidiaries, Patriot Rail Holdings LLC (PRH) and...

78 FR 2388 - Combined Notice of Filings #1

Federal Register 2010, 2011, 2012, 2013, 2014

2013-01-11

...-2401-002; ER10-2402-002; ER11- 3414-004; ER10-2403-002. Applicants: Blue Canyon Windpower LLC, Blue Canyon Windpower II LLC, Blue Canyon Windpower V LLC, Blue Canyon Windpower VI LLC, Cloud County Wind Farm, LLC. Description: Blue Canyon Windpower LLC, et al. submits Updated Market Power Analysis for...

76 FR 76393 - Combined Notice of Filings #1

Federal Register 2010, 2011, 2012, 2013, 2014

2011-12-07

...; ER11-4499-002; ER11-4500-002; ER11- 4507-002; ER11-4501-002. Applicants: Smoky Hills Wind Farm, LLC, Smoky Hills Wind Project II, LLC, Enel Stillwater, LLC, Caney River Wind Project, LLC, Canastota Windpower, LLC. Description: Notice of Non-Material Change in Status re Smoky Hills Wind Farm, LLC, et al...

76 FR 36532 - Iberdrola Renewables, Inc., PacifiCorp, NextEra Energy Resources, LLC, Invenergy Wind North...

Federal Register 2010, 2011, 2012, 2013, 2014

2011-06-22

... Renewables, Inc., PacifiCorp, NextEra Energy Resources, LLC, Invenergy Wind North America LLC, Horizon Wind...), Iberdrola Renewables, Inc., PacifiCorp, NextEra Energy Resources, LLC, Invenergy Wind North America LLC, and Horizon Wind Energy LLC (Complainants) filed a formal complaint against Bonneville Power Administration...

75 FR 49527 - Caps Visual Communications, LLC; Black Dot Group; Formerly Known as Caps Group Acquisition, LLC...

Federal Register 2010, 2011, 2012, 2013, 2014

2010-08-13

... DEPARTMENT OF LABOR Employment and Training Administration [TA-W-74,195] Caps Visual Communications, LLC; Black Dot Group; Formerly Known as Caps Group Acquisition, LLC Chicago, IL; Amended... of Caps Visual Communications, LLC, Black Dot Group, formerly known as Caps Group Acquisition, LLC...

77 FR 25715 - Mississippi 8 Hydro, LLC; FFP Project 98, LLC, ; Notice Announcing Filing Priority for...

Federal Register 2010, 2011, 2012, 2013, 2014

2012-05-01

... DEPARTMENT OF ENERGY Federal Energy Regulatory Commission [Project No., 13010-002; Project No., 14272-000] Mississippi 8 Hydro, LLC; FFP Project 98, LLC, ; Notice Announcing Filing Priority for.... Mississippi 8 Hydro, LLC--Project No. 13010-002. 2. FFP Project 98, LLC--Project No. 14272-000. Dated: April...

78 FR 3425 - Filiquarian Publishing, LLC; Choice Level, LLC; and Joshua Linsk; Analysis of Proposed Consent...

Federal Register 2010, 2011, 2012, 2013, 2014

2013-01-16

... FEDERAL TRADE COMMISSION [File No. 112 3195] Filiquarian Publishing, LLC; Choice Level, LLC; and Joshua Linsk; Analysis of Proposed Consent Order To Aid Public Comment AGENCY: Federal Trade Commission... approval, an agreement containing a consent order from Filiquarian Publishing, LLC; Choice Level, LLC; and...

76 FR 73612 - Lock Hydro Friends Fund XVIII; Upper Hydroelectric LLC; FFP Project 95 LLC; Riverbank Hydro No...

Federal Register 2010, 2011, 2012, 2013, 2014

2011-11-29

... DEPARTMENT OF ENERGY Federal Energy Regulatory Commission [Project Nos. 14261-000; 14268-000; 14277-000; 14281-000] Lock Hydro Friends Fund XVIII; Upper Hydroelectric LLC; FFP Project 95 LLC... Friends Fund XVIII (Lock Hydro), Upper Hydroelectric LLC (Upper Hydro), Riverbank Hydro No. 25 LLC...

78 FR 2389 - Combined Notice of Filings #1

Federal Register 2010, 2011, 2012, 2013, 2014

2013-01-11

...; ER10-2814-002; ER10-3026-002. Applicants: Copper Mountain Solar 1, LLC, Copper Mountain Solar 2, LLC, Energia Sierra Juarez U.S., LLC, Mesquite Power, LLC, Mesquite Solar 1, LLC, San Diego Gas & Electric... Power Analysis for the Southwest Power Pool, Inc. Region of Copper Mountain Solar 1, LLC, et. al. Filed...

78 FR 37214 - Transcontinental Gas Pipe Line Company, LLC; Notice of Availability of the Environmental...

Federal Register 2010, 2011, 2012, 2013, 2014

2013-06-20

... constitute a major federal action significantly affecting the quality of the human environment. The U.S. Army... station, line heaters, and pig \\1\\ receiver at the terminus of the Brunswick Lateral; and \\1\\ A ``pig'' is...

Flexible workflow sharing and execution services for e-scientists

NASA Astrophysics Data System (ADS)

Kacsuk, Péter; Terstyanszky, Gábor; Kiss, Tamas; Sipos, Gergely

2013-04-01

The sequence of computational and data manipulation steps required to perform a specific scientific analysis is called a workflow. Workflows that orchestrate data and/or compute intensive applications on Distributed Computing Infrastructures (DCIs) recently became standard tools in e-science. At the same time the broad and fragmented landscape of workflows and DCIs slows down the uptake of workflow-based work. The development, sharing, integration and execution of workflows is still a challenge for many scientists. The FP7 "Sharing Interoperable Workflow for Large-Scale Scientific Simulation on Available DCIs" (SHIWA) project significantly improved the situation, with a simulation platform that connects different workflow systems, different workflow languages, different DCIs and workflows into a single, interoperable unit. The SHIWA Simulation Platform is a service package, already used by various scientific communities, and used as a tool by the recently started ER-flow FP7 project to expand the use of workflows among European scientists. The presentation will introduce the SHIWA Simulation Platform and the services that ER-flow provides based on the platform to space and earth science researchers. The SHIWA Simulation Platform includes: 1. SHIWA Repository: A database where workflows and meta-data about workflows can be stored. The database is a central repository to discover and share workflows within and among communities . 2. SHIWA Portal: A web portal that is integrated with the SHIWA Repository and includes a workflow executor engine that can orchestrate various types of workflows on various grid and cloud platforms. 3. SHIWA Desktop: A desktop environment that provides similar access capabilities than the SHIWA Portal, however it runs on the users' desktops/laptops instead of a portal server. 4. Workflow engines: the ASKALON, Galaxy, GWES, Kepler, LONI Pipeline, MOTEUR, Pegasus, P-GRADE, ProActive, Triana, Taverna and WS-PGRADE workflow engines are already integrated with the execution engine of the SHIWA Portal. Other engines can be added when required. Through the SHIWA Portal one can define and run simulations on the SHIWA Virtual Organisation, an e-infrastructure that gathers computing and data resources from various DCIs, including the European Grid Infrastructure. The Portal via third party workflow engines provides support for the most widely used academic workflow engines and it can be extended with other engines on demand. Such extensions translate between workflow languages and facilitate the nesting of workflows into larger workflows even when those are written in different languages and require different interpreters for execution. Through the workflow repository and the portal lonely scientists and scientific collaborations can share and offer workflows for reuse and execution. Given the integrated nature of the SHIWA Simulation Platform the shared workflows can be executed online, without installing any special client environment and downloading workflows. The FP7 "Building a European Research Community through Interoperable Workflows and Data" (ER-flow) project disseminates the achievements of the SHIWA project and use these achievements to build workflow user communities across Europe. ER-flow provides application supports to research communities within and beyond the project consortium to develop, share and run workflows with the SHIWA Simulation Platform.

75 FR 71116 - Combined Notice of Filings # 1

Federal Register 2010, 2011, 2012, 2013, 2014

2010-11-22

... Holding, LLC; Cogen Technologies Linden Venture, L.P.; Fox Energy Company LLC; Birchwood Power Partners, L...; Somerset Windpower, LLC; Story Wind, LLC; Victory Garden Phase IV, LLC; Waymart Wind Farm, L.P.; Wessington...

Scientist-Centered Workflow Abstractions via Generic Actors, Workflow Templates, and Context-Awareness for Groundwater Modeling and Analysis

DOE Office of Scientific and Technical Information (OSTI.GOV)

Chin, George; Sivaramakrishnan, Chandrika; Critchlow, Terence J.

2011-07-04

A drawback of existing scientific workflow systems is the lack of support to domain scientists in designing and executing their own scientific workflows. Many domain scientists avoid developing and using workflows because the basic objects of workflows are too low-level and high-level tools and mechanisms to aid in workflow construction and use are largely unavailable. In our research, we are prototyping higher-level abstractions and tools to better support scientists in their workflow activities. Specifically, we are developing generic actors that provide abstract interfaces to specific functionality, workflow templates that encapsulate workflow and data patterns that can be reused and adaptedmore » by scientists, and context-awareness mechanisms to gather contextual information from the workflow environment on behalf of the scientist. To evaluate these scientist-centered abstractions on real problems, we apply them to construct and execute scientific workflows in the specific domain area of groundwater modeling and analysis.« less

76 FR 66709 - Trunkline Gas Company, LLC, Sea Robin Pipeline Company, LLC; Notice of Application

Federal Register 2010, 2011, 2012, 2013, 2014

2011-10-27

... Company, LLC, Sea Robin Pipeline Company, LLC; Notice of Application Take notice that on October 7, 2011, Trunkline Gas Company, LLC (Trunkline) and Sea Robin Pipeline Company, LLC (Sea Robin), together referred to...), for permission and approval for Trunkline to abandon by sale to Sea Robin and for Sea Robin to acquire...

77 FR 17469 - Combined Notice of Filings #1

Federal Register 2010, 2011, 2012, 2013, 2014

2012-03-26

...-4178-001; ER11-4179-001; ER11- 39-003. Applicants: Flat Water Wind Farm, LLC, Roth Rock Wind Farm, LLC, TPW Petersburg, LLC, Roth Rock North Wind Farm, LLC. Description: Clarification of TPW Petersburg, LLC...-5181. Comments Due: 5 p.m. ET 4/6/12. Docket Numbers: ER12-1198-001. Applicants: Solano 3 Wind LLC...

77 FR 63874 - Birds Eye Foods, LLC; Fulton, NY Plant; A Wholly-Owned Subsidiary of Pinnacle Foods Group LLC...

Federal Register 2010, 2011, 2012, 2013, 2014

2012-10-17

... DEPARTMENT OF LABOR Employment and Training Administration [TA-W-81,009] Birds Eye Foods, LLC... workers of Birds Eye Foods, LLC, Fulton, NY Plant, a wholly-owned subsidiary of Pinnacle Foods Group LLC... Eye Foods, LLC. The Department has determined that these workers were sufficiently under the control...

77 FR 10740 - Riverbank Hydro No. 22, LLC; FFP Project 93, LLC; Notice Announcing Preliminary Permit Drawing

Federal Register 2010, 2011, 2012, 2013, 2014

2012-02-23

... DEPARTMENT OF ENERGY Federal Energy Regulatory Commission [Project Nos. 14269-000; 14270-000] Riverbank Hydro No. 22, LLC; FFP Project 93, LLC; Notice Announcing Preliminary Permit Drawing The... Riverbank Hydro No. 22, LLC for Project No. 14269-000, and FFP Project 93, LLC for Project No. 14270-000. \\1...

78 FR 62344 - Sabine Pass Liquefaction Expansion, LLC, Sabine Pass Liquefaction, LLC, and Sabine Pass LNG, L.P...

Federal Register 2010, 2011, 2012, 2013, 2014

2013-10-18

.... CP13-553-000] Sabine Pass Liquefaction Expansion, LLC, Sabine Pass Liquefaction, LLC, and Sabine Pass... 30, 2013, Sabine Pass Liquefaction Expansion, LLC, Sabine Pass Liquefaction, LLC, and Sabine Pass LNG, L.P. (collectively referred to as Sabine Pass) filed with the Federal Energy Regulatory Commission...

76 FR 67171 - Solia 7 Hydroelectric LLC; Riverbank Hydro No. 20 LLC; Lock Hydro Friends Fund XLIII; FFP Project...

Federal Register 2010, 2011, 2012, 2013, 2014

2011-10-31

... DEPARTMENT OF ENERGY Federal Energy Regulatory Commission [Project Nos. 14163-000; 14174-000; 14181-000; 14195-000] Solia 7 Hydroelectric LLC; Riverbank Hydro No. 20 LLC; Lock Hydro Friends Fund... LLC (Solia), and Riverbank Hydro No. 20 LLC (Riverbank), and on May 3, 2011, Lock Hydro Friends Fund...

78 FR 25784 - Keenesburg Direct Railroad, LLC-Acquisition and Operation Exemption-J D East, LLC

Federal Register 2010, 2011, 2012, 2013, 2014

2013-05-02

... DEPARTMENT OF TRANSPORTATION Surface Transportation Board [Docket No. FD 35735] Keenesburg Direct Railroad, LLC--Acquisition and Operation Exemption--J D East, LLC Keenesburg Direct Railroad, LLC (KDR), a noncarrier, has filed a verified notice of exemption under 49 CFR 1150.31 to acquire from J D East, LLC (JDE...

78 FR 11928 - Self-Regulatory Organizations; New York Stock Exchange LLC; NYSE MKT LLC; Order Instituting...

Federal Register 2010, 2011, 2012, 2013, 2014

2013-02-20

...-2012-58] Self-Regulatory Organizations; New York Stock Exchange LLC; NYSE MKT LLC; Order Instituting.... I. Introduction On October 26, 2012, the New York Stock Exchange LLC (``NYSE'') and NYSE MKT LLC... of the market, the Floor broker may liquidate or cover the position only pursuant to a new order...

Lewis lung carcinoma progression is facilitated by TIG-3 fibroblast cells.

PubMed

Yamauchi, Yoshikane; Izumi, Yotaro; Asakura, Keisuke; Kawai, Kenji; Wakui, Masatoshi; Ohmura, Mitsuyo; Suematsu, Makoto; Nomori, Hiroaki

2013-09-01

The interactions of tumor cells with stromal fibroblasts influence tumor biology, but the exact mechanisms involved are still unclear. In the present study, we evaluated the effects of a human lung fibroblast cell line, TIG-3, on Lewis lung carcinoma (LLC) cells both in vitro and in vivo. LLC and TIG-3 cells were co-cultured/co-implanted in vitro and in vivo. Cell invasion was assayed. Local tumor growth, as well as lung metastasis, were evaluated after subcutaneous cell co-implantation into NOD/SCID/γ-null (NOG) mice. LLC, and TIG-3 cells were pre-treated with either SB431542, a small molecule TGF-β receptor antagonist, or siRNA for transforming growth factor (TGF)-β before co-culture or co-implantation, and the effects of pre-treatments were compared both in cell culture and in mice. Subcutaneous LLC tumor growth (L group) in NOG mice was significantly increased by co-implantation of TIG-3 cells (L+T group) at four weeks. The number of macroscopic lung metastases was also significantly increased in the L+T group in comparison to the L group. In vitro cell invasion was significantly increased in the L+T group in comparison to the L group. In vitro expression of phosphorylated-SMAD3 was significantly increased in the L+T group in comparison to the L group. Furthermore, pre-treatment with either SB431542 or siRNA for TGF-β reduced the invasiveness both in culture and in mice. This study suggested that in vitro as well as in vivo progression of LLC was facilitated by co-culture/co-implantation with TIG-3 cells, and that this process was at least in part dependent on TGF-β-mediated interactions.

Tavaxy: integrating Taverna and Galaxy workflows with cloud computing support.

PubMed

Abouelhoda, Mohamed; Issa, Shadi Alaa; Ghanem, Moustafa

2012-05-04

Over the past decade the workflow system paradigm has evolved as an efficient and user-friendly approach for developing complex bioinformatics applications. Two popular workflow systems that have gained acceptance by the bioinformatics community are Taverna and Galaxy. Each system has a large user-base and supports an ever-growing repository of application workflows. However, workflows developed for one system cannot be imported and executed easily on the other. The lack of interoperability is due to differences in the models of computation, workflow languages, and architectures of both systems. This lack of interoperability limits sharing of workflows between the user communities and leads to duplication of development efforts. In this paper, we present Tavaxy, a stand-alone system for creating and executing workflows based on using an extensible set of re-usable workflow patterns. Tavaxy offers a set of new features that simplify and enhance the development of sequence analysis applications: It allows the integration of existing Taverna and Galaxy workflows in a single environment, and supports the use of cloud computing capabilities. The integration of existing Taverna and Galaxy workflows is supported seamlessly at both run-time and design-time levels, based on the concepts of hierarchical workflows and workflow patterns. The use of cloud computing in Tavaxy is flexible, where the users can either instantiate the whole system on the cloud, or delegate the execution of certain sub-workflows to the cloud infrastructure. Tavaxy reduces the workflow development cycle by introducing the use of workflow patterns to simplify workflow creation. It enables the re-use and integration of existing (sub-) workflows from Taverna and Galaxy, and allows the creation of hybrid workflows. Its additional features exploit recent advances in high performance cloud computing to cope with the increasing data size and complexity of analysis.The system can be accessed either through a cloud-enabled web-interface or downloaded and installed to run within the user's local environment. All resources related to Tavaxy are available at http://www.tavaxy.org.

Data Curation: Improving Environmental Health Data Quality.

PubMed

Yang, Lin; Li, Jiao; Hou, Li; Qian, Qing

2015-01-01

With the growing recognition of the influence of climate change on human health, scientists' attention to analyzing the relationship between meteorological factors and adverse health effects. However, the paucity of high quality integrated data is one of the great challenges, especially when scientific studies rely on data-intensive computing. This paper aims to design an appropriate curation process to address this problem. We present a data curation workflow that: (i) follows the guidance of DCC Curation Lifecycle Model; (ii) combines manual curation with automatic curation; (iii) and solves environmental health data curation problem. The workflow was applied to a medical knowledge service system and showed that it was capable of improving work efficiency and data quality.

Human Systems Integration (HSI) Associated Development Activities in Japan

DTIC Science & Technology

2008-06-12

machine learning and data mining methods. The continuous effort ( KAIZEN ) to improve the analysis phases are illustrated in Figure 14. Although there...model Extraction of a workflow Extraction of a control rule Variation analysis and improvement Plant operation KAIZEN Fig. 14

78 FR 40444 - Notice of Telephonic Prehearing Conference

Federal Register 2010, 2011, 2012, 2013, 2014

2013-07-05

... OBERTON HOLDINGS, LLC; ZEN MAGNETS, LLC; and STAR NETWORKS USA, LLC; CPSC Docket No. 12-1; CPSC Docket No.... Japha, Esq., Counsel for ZEN MAGNETS, LLC and STAR NETWORKS USA, LLC shall be contacted by a third party...

76 FR 78641 - Cedar Creek Wind Energy, LLC, Milford Wind Corridor Phase I, LLC; Notice of Filing

Federal Register 2010, 2011, 2012, 2013, 2014

2011-12-19

... DEPARTMENT OF ENERGY Federal Energy Regulatory Commission [Docket No. RC11-1-002; Docket No. RC11-2-002] Cedar Creek Wind Energy, LLC, Milford Wind Corridor Phase I, LLC; Notice of Filing Take...) June 16, 2011 Order.\\1\\ \\1\\ Cedar Creek Wind Energy, LLC and Milford Wind Corridor Phase I, LLC, 135...

76 FR 67169 - Solia 9 Hydroelectric LLC Riverbank Hydro No. 17 LLC Lock Hydro Friends Fund XLI FFP Project 54...

Federal Register 2010, 2011, 2012, 2013, 2014

2011-10-31

... DEPARTMENT OF ENERGY Federal Energy Regulatory Commission [Project Nos. 14162-000; 14173-000; 14182-000; 14192-000] Solia 9 Hydroelectric LLC Riverbank Hydro No. 17 LLC Lock Hydro Friends Fund XLI... LLC (Solia), and Riverbank Hydro No. 17 LLC (Riverbank), and on May 3, 2011, Lock Hydro Friends Fund...

75 FR 16091 - Glen Falls Hydro, LLC; Essex Energy Partners, LLC; Notice of Application for Transfer of License...

Federal Register 2010, 2011, 2012, 2013, 2014

2010-03-31

... DEPARTMENT OF ENERGY Federal Energy Regulatory Commission [Project No. 11143-020] Glen Falls Hydro, LLC; Essex Energy Partners, LLC; Notice of Application for Transfer of License, and Soliciting Comments and Motions To Intervene March 24, 2010. On March 8, 2010, Glen Falls Hydro, LLC (transferor) and Essex Energy Partners, LLC (transferee) filed...

76 FR 67775 - Citadel LLC (formerly Citadel Investment Group, L.L.C.) and CEIF LLC; Notice of Application

Federal Register 2010, 2011, 2012, 2013, 2014

2011-11-02

... SECURITIES AND EXCHANGE COMMISSION [Investment Company Act Release No. 29851; File No. 813-00377] Citadel LLC (formerly Citadel Investment Group, L.L.C.) and CEIF LLC; Notice of Application October 27... order under sections 6(b) and 6(e) of the Investment Company Act of 1940 (the ``Act'') granting an...

77 FR 27763 - Quantum Choctaw Power, LLC, USG Nevada LLC, et al.; Notice of Effectiveness of Exempt Wholesale...

Federal Register 2010, 2011, 2012, 2013, 2014

2012-05-11

... DEPARTMENT OF ENERGY Federal Energy Regulatory Commission [Docket Nos. EG12-31-000; EG12-32-000; et al.] Quantum Choctaw Power, LLC, USG Nevada LLC, et al.; Notice of Effectiveness of Exempt Wholesale Generator Status Docket Nos. Quantum Choctaw Power, LLC EG12-31-000 USG Nevada LLC EG12-32-000...

77 FR 31347 - Tennessee Gas Pipeline Company, L.L.C.; Notice of Availability of the Environmental Assessment...

Federal Register 2010, 2011, 2012, 2013, 2014

2012-05-25

... a major federal action significantly affecting the quality of the human environment. The U.S. Army... equipment along Loop 313 including a pig launcher; \\2\\ and \\2\\ A ``pig'' is a tool that the pipeline company...

Inferring Clinical Workflow Efficiency via Electronic Medical Record Utilization

PubMed Central

Chen, You; Xie, Wei; Gunter, Carl A; Liebovitz, David; Mehrotra, Sanjay; Zhang, He; Malin, Bradley

2015-01-01

Complexity in clinical workflows can lead to inefficiency in making diagnoses, ineffectiveness of treatment plans and uninformed management of healthcare organizations (HCOs). Traditional strategies to manage workflow complexity are based on measuring the gaps between workflows defined by HCO administrators and the actual processes followed by staff in the clinic. However, existing methods tend to neglect the influences of EMR systems on the utilization of workflows, which could be leveraged to optimize workflows facilitated through the EMR. In this paper, we introduce a framework to infer clinical workflows through the utilization of an EMR and show how such workflows roughly partition into four types according to their efficiency. Our framework infers workflows at several levels of granularity through data mining technologies. We study four months of EMR event logs from a large medical center, including 16,569 inpatient stays, and illustrate that over approximately 95% of workflows are efficient and that 80% of patients are on such workflows. At the same time, we show that the remaining 5% of workflows may be inefficient due to a variety of factors, such as complex patients. PMID:26958173

Workflow management systems in radiology

NASA Astrophysics Data System (ADS)

Wendler, Thomas; Meetz, Kirsten; Schmidt, Joachim

1998-07-01

In a situation of shrinking health care budgets, increasing cost pressure and growing demands to increase the efficiency and the quality of medical services, health care enterprises are forced to optimize or complete re-design their processes. Although information technology is agreed to potentially contribute to cost reduction and efficiency improvement, the real success factors are the re-definition and automation of processes: Business Process Re-engineering and Workflow Management. In this paper we discuss architectures for the use of workflow management systems in radiology. We propose to move forward from information systems in radiology (RIS, PACS) to Radiology Management Systems, in which workflow functionality (process definitions and process automation) is implemented through autonomous workflow management systems (WfMS). In a workflow oriented architecture, an autonomous workflow enactment service communicates with workflow client applications via standardized interfaces. In this paper, we discuss the need for and the benefits of such an approach. The separation of workflow management system and application systems is emphasized, and the consequences that arise for the architecture of workflow oriented information systems. This includes an appropriate workflow terminology, and the definition of standard interfaces for workflow aware application systems. Workflow studies in various institutions have shown that most of the processes in radiology are well structured and suited for a workflow management approach. Numerous commercially available Workflow Management Systems (WfMS) were investigated, and some of them, which are process- oriented and application independent, appear suitable for use in radiology.

77 FR 56834 - Notice of Effectiveness of Exempt Wholesale Generator Status

Federal Register 2010, 2011, 2012, 2013, 2014

2012-09-14

... Wholesale Generator Status Docket Nos. AltaGas Renewable Energy Colorado LLC.. EG12-70-000 Patton Wind Farm, LLC EG12-71-000 Big Savage, LLC EG12-72-000 Pacific Wind Lessee, LLC EG12-73-000 High Majestic...-000 Spearville 3, LLC EG12-77-000 NaturEner Rim Rock Wind Energy, LLC.... EG12-78-000 NaturEner...

78 FR 5798 - Grouse Creek Wind Park, LLC, Grouse Creek Wind Park II, LLC; Notice of Petition for Enforcement

Federal Register 2010, 2011, 2012, 2013, 2014

2013-01-28

... DEPARTMENT OF ENERGY Federal Energy Regulatory Commission [Docket Nos. El13-39-000, QF11-32-001, QF11-33-001] Grouse Creek Wind Park, LLC, Grouse Creek Wind Park II, LLC; Notice of Petition for... Policies Act of 1978 (PURPA), Grouse Creek Wind Park, LLC and Grouse Creek Wind Park II, LLC filed a...

75 FR 17706 - FPL Energy Maine Hydro LLC, Complainant v. Great Lakes Hydro America LLC Rumford Falls Hydro LLC...

Federal Register 2010, 2011, 2012, 2013, 2014

2010-04-07

... DEPARTMENT OF ENERGY Federal Energy Regulatory Commission [Docket No. EL10-53-000] FPL Energy Maine Hydro LLC, Complainant v. Great Lakes Hydro America LLC Rumford Falls Hydro LLC, Respondents; Notice of Complaint March 29, 2010. Take notice that on March 26, 2010, pursuant to section 206 of the Federal Energy Regulatory Commission's (Commission...

77 FR 70434 - Petal Gas Storage, L.L.C., Hattiesburg Industrial Gas Sales, L.L.C.; Notice of Offer of Settlement

Federal Register 2010, 2011, 2012, 2013, 2014

2012-11-26

... DEPARTMENT OF ENERGY Federal Energy Regulatory Commission [Docket No. CP12-464-000] Petal Gas Storage, L.L.C., Hattiesburg Industrial Gas Sales, L.L.C.; Notice of Offer of Settlement Take notice that on November 8, 2012, Petal Gas Storage, L.L.C. (Petal) and Hattiesburg Industrial Gas Sales, L.L.C...

Driving CT developments the last mile: case examples of successful and somewhat less successful translations into clinical practice

NASA Astrophysics Data System (ADS)

Sodickson, Aaron D.

2017-03-01

CT technology has advanced rapidly in recent years, yet not all innovations translate readily into clinical practice. Technology advances must meet certain key requirements to make it into routine use: They must provide a well-defined clinical benefit. They must be easy to use and integrate readily into existing workflows, or better still, further streamline these workflows. These requirements heavily favor fully integrated or automated solutions that remove the human factor and provide a reproducible output independent of operator skill level. Further, to achieve these aims, collaboration with the ultimate end users is needed as early as possible in the development cycle, not just at the point of product testing. Technology innovators are encouraged to engage such collaborators even at early stages of feature or product definition. This manuscript highlights these concepts through exploration of challenging areas in CT imaging in an Emergency Department setting. Technique optimization for pulmonary embolus CT is described as an example of successful integration of multiple advances in radiation dose reduction and imaging speed. The typical workflow of a trauma "pan-scan" (incorporating scans from head through pelvis) is described to highlight workflow challenges and opportunities for improvement. Finally, Dual Energy CT is discussed to highlight the undeniable clinical value of the material characterization it provides, yet also its surprisingly slow integration into routine use beyond early adopters.

78 FR 3042 - J.P. Morgan Securities LLC, et al.; Notice of Application and Temporary Order

Federal Register 2010, 2011, 2012, 2013, 2014

2013-01-15

... Management Inc. (``BSAM''), Bear Stearns Health Innoventures Management, L.L.C. (``BSHIM''), BSCGP Inc. (``BSGCP''), Constellation Growth Capital LLC (``Constellation''), Constellation Ventures Management II, LLC (``Constellation II''), Highbridge Capital Management, LLC (``Highbridge''), JF International...

78 FR 23243 - Combined Notice of Filings #1

Federal Register 2010, 2011, 2012, 2013, 2014

2013-04-18

...: EDF Trading North America, LLC, EDF Industrial Power Services (NY), LLC, EDF Industrial Power Services (IL), LLC, EDF Industrial Power Services (CA), LLC, Tanner Street Generation, LLC Description: Notice... England Power Pool Participants Committee, ISO New England Inc. Description: Regulation Market Opportunity...

Bioinformatics workflows and web services in systems biology made easy for experimentalists.

PubMed

Jimenez, Rafael C; Corpas, Manuel

2013-01-01

Workflows are useful to perform data analysis and integration in systems biology. Workflow management systems can help users create workflows without any previous knowledge in programming and web services. However the computational skills required to build such workflows are usually above the level most biological experimentalists are comfortable with. In this chapter we introduce workflow management systems that reuse existing workflows instead of creating them, making it easier for experimentalists to perform computational tasks.

A novel image processing workflow for the in vivo quantification of skin microvasculature using dynamic optical coherence tomography.

PubMed

Zugaj, D; Chenet, A; Petit, L; Vaglio, J; Pascual, T; Piketty, C; Bourdes, V

2018-02-04

Currently, imaging technologies that can accurately assess or provide surrogate markers of the human cutaneous microvessel network are limited. Dynamic optical coherence tomography (D-OCT) allows the detection of blood flow in vivo and visualization of the skin microvasculature. However, image processing is necessary to correct images, filter artifacts, and exclude irrelevant signals. The objective of this study was to develop a novel image processing workflow to enhance the technical capabilities of D-OCT. Single-center, vehicle-controlled study including healthy volunteers aged 18-50 years. A capsaicin solution was applied topically on the subject's forearm to induce local inflammation. Measurements of capsaicin-induced increase in dermal blood flow, within the region of interest, were performed by laser Doppler imaging (LDI) (reference method) and D-OCT. Sixteen subjects were enrolled. A good correlation was shown between D-OCT and LDI, using the image processing workflow. Therefore, D-OCT offers an easy-to-use alternative to LDI, with good repeatability, new robust morphological features (dermal-epidermal junction localization), and quantification of the distribution of vessel size and changes in this distribution induced by capsaicin. The visualization of the vessel network was improved through bloc filtering and artifact removal. Moreover, the assessment of vessel size distribution allows a fine analysis of the vascular patterns. The newly developed image processing workflow enhances the technical capabilities of D-OCT for the accurate detection and characterization of microcirculation in the skin. A direct clinical application of this image processing workflow is the quantification of the effect of topical treatment on skin vascularization. © 2018 The Authors. Skin Research and Technology Published by John Wiley & Sons Ltd.

Workflow for high-content, individual cell quantification of fluorescent markers from universal microscope data, supported by open source software.

PubMed

Stockwell, Simon R; Mittnacht, Sibylle

2014-12-16

Advances in understanding the control mechanisms governing the behavior of cells in adherent mammalian tissue culture models are becoming increasingly dependent on modes of single-cell analysis. Methods which deliver composite data reflecting the mean values of biomarkers from cell populations risk losing subpopulation dynamics that reflect the heterogeneity of the studied biological system. In keeping with this, traditional approaches are being replaced by, or supported with, more sophisticated forms of cellular assay developed to allow assessment by high-content microscopy. These assays potentially generate large numbers of images of fluorescent biomarkers, which enabled by accompanying proprietary software packages, allows for multi-parametric measurements per cell. However, the relatively high capital costs and overspecialization of many of these devices have prevented their accessibility to many investigators. Described here is a universally applicable workflow for the quantification of multiple fluorescent marker intensities from specific subcellular regions of individual cells suitable for use with images from most fluorescent microscopes. Key to this workflow is the implementation of the freely available Cell Profiler software(1) to distinguish individual cells in these images, segment them into defined subcellular regions and deliver fluorescence marker intensity values specific to these regions. The extraction of individual cell intensity values from image data is the central purpose of this workflow and will be illustrated with the analysis of control data from a siRNA screen for G1 checkpoint regulators in adherent human cells. However, the workflow presented here can be applied to analysis of data from other means of cell perturbation (e.g., compound screens) and other forms of fluorescence based cellular markers and thus should be useful for a wide range of laboratories.

76 FR 67174 - Arkansas Electric Cooperative Corp., Riverbank Hydro No. 9 LLC, Solia 3 Hydroelectric LLC, Lock...

Federal Register 2010, 2011, 2012, 2013, 2014

2011-10-31

... Hydroelectric LLC, Lock Hydro Friends Fund XLV, FFP Project 2 LLC; Notice of Competing Preliminary Permit... (Riverbank) and Solia 3 Hydroelectric LLC (Solia) and on May 3, 2011, Lock Hydro Friends Fund XLV (Lock Hydro...

75 FR 12533 - Combined Notice Of Filings #1

Federal Register 2010, 2011, 2012, 2013, 2014

2010-03-16

....; R.E. Ginna Nuclear Power Plant, LLC; Constellation Energy Commodities Group; Constellation Energy Commodities Group Maine; Raven Three, LLC; Raven Two, LLC; Raven One, LLC; Calvert Cliffs Nuclear Power Plant LLC. Description: Calvert Cliffs Nuclear Power Plant submits Substitute First Revised Sheet 1 et al...

77 FR 2972 - Thunder Bay Power Company, Thunder Bay Power, LLC, et al.

Federal Register 2010, 2011, 2012, 2013, 2014

2012-01-20

... Hydro, LLC. Dayton Fox River, LaSalle County, IL. P-2347-049 Midwest Hydro, Inc. Midwest Hydro, LLC... FR, Neenah Paper FR, Middle Appleton Dam Lower Fox River, LLC and N.E.W. LLC and N.E.W. Outagamie...

Tavaxy: Integrating Taverna and Galaxy workflows with cloud computing support

PubMed Central

2012-01-01

Background Over the past decade the workflow system paradigm has evolved as an efficient and user-friendly approach for developing complex bioinformatics applications. Two popular workflow systems that have gained acceptance by the bioinformatics community are Taverna and Galaxy. Each system has a large user-base and supports an ever-growing repository of application workflows. However, workflows developed for one system cannot be imported and executed easily on the other. The lack of interoperability is due to differences in the models of computation, workflow languages, and architectures of both systems. This lack of interoperability limits sharing of workflows between the user communities and leads to duplication of development efforts. Results In this paper, we present Tavaxy, a stand-alone system for creating and executing workflows based on using an extensible set of re-usable workflow patterns. Tavaxy offers a set of new features that simplify and enhance the development of sequence analysis applications: It allows the integration of existing Taverna and Galaxy workflows in a single environment, and supports the use of cloud computing capabilities. The integration of existing Taverna and Galaxy workflows is supported seamlessly at both run-time and design-time levels, based on the concepts of hierarchical workflows and workflow patterns. The use of cloud computing in Tavaxy is flexible, where the users can either instantiate the whole system on the cloud, or delegate the execution of certain sub-workflows to the cloud infrastructure. Conclusions Tavaxy reduces the workflow development cycle by introducing the use of workflow patterns to simplify workflow creation. It enables the re-use and integration of existing (sub-) workflows from Taverna and Galaxy, and allows the creation of hybrid workflows. Its additional features exploit recent advances in high performance cloud computing to cope with the increasing data size and complexity of analysis. The system can be accessed either through a cloud-enabled web-interface or downloaded and installed to run within the user's local environment. All resources related to Tavaxy are available at http://www.tavaxy.org. PMID:22559942

76 FR 2365 - Notice of Effectiveness of Exempt Wholesale Generator Status

Federal Register 2010, 2011, 2012, 2013, 2014

2011-01-13

... Wholesale Generator Status January 6, 2011. Docket No. Ashtabula Wind III, LLC EG11-1-000 Iberdrola Renewables, Inc EG11-2-000 Flat Water Wind Farm, LLC EG11-3-000 Wildorado Wind Two, LLC EG11-4-000 Sandy Ridge Wind, LLC EG11-5-000 AES ES Deepwater, LLC EG11-6-000 Elk City II Wind, LLC EG11-7-000 Take notice...

77 FR 34031 - Petal Gas Storage, L.L.C., Hattiesburg Industrial Gas Sales, L.L.C.; Notice of Application

Federal Register 2010, 2011, 2012, 2013, 2014

2012-06-08

... Storage, L.L.C., Hattiesburg Industrial Gas Sales, L.L.C.; Notice of Application Take notice that on May 21, 2012, Petal Gas Storage, L.L.C. (Petal) and Hattiesburg Industrial Gas Sales, L.L.C. (Hattiesburg... pursuant to sections 7(c) and 7(b) of the Natural Gas Act (NGA), for authorization for Petal to acquire the...

77 FR 56693 - Self-Regulatory Organizations; New York Stock Exchange LLC; Notice of Filing and Immediate...

Federal Register 2010, 2011, 2012, 2013, 2014

2012-09-13

...'' Reflecting the Recent Name Change of NYSE Amex LLC to NYSE MKT LLC September 7, 2012. Pursuant to Section 19...'' with ``NYSE MKT'' to reflect the recent name change of NYSE Amex LLC to NYSE MKT LLC. The text of the... references to ``NYSE Amex'' with ``NYSE MKT'' to reflect the recent name change of NYSE Amex LLC to NYSE MKT...

Climate Science Performance, Data and Productivity on Titan

DOE Office of Scientific and Technical Information (OSTI.GOV)

Mayer, Benjamin W; Worley, Patrick H; Gaddis, Abigail L

2015-01-01

Climate Science models are flagship codes for the largest of high performance computing (HPC) resources, both in visibility, with the newly launched Department of Energy (DOE) Accelerated Climate Model for Energy (ACME) effort, and in terms of significant fractions of system usage. The performance of the DOE ACME model is captured with application level timers and examined through a sizeable run archive. Performance and variability of compute, queue time and ancillary services are examined. As Climate Science advances in the use of HPC resources there has been an increase in the required human and data systems to achieve programs goals.more » A description of current workflow processes (hardware, software, human) and planned automation of the workflow, along with historical and projected data in motion and at rest data usage, are detailed. The combination of these two topics motivates a description of future systems requirements for DOE Climate Modeling efforts, focusing on the growth of data storage and network and disk bandwidth required to handle data at an acceptable rate.« less

A Fast Solution to NGS Library Prep with Low Nanogram DNA Input

PubMed Central

Liu, Pingfang; Lohman, Gregory J.S.; Cantor, Eric; Langhorst, Bradley W.; Yigit, Erbay; Apone, Lynne M.; Munafo, Daniela B.; Stewart, Fiona J.; Evans, Thomas C.; Nichols, Nicole; Dimalanta, Eileen T.; Davis, Theodore B.; Sumner, Christine

2013-01-01

Next Generation Sequencing (NGS) has significantly impacted human genetics, enabling a comprehensive characterization of the human genome as well as a better understanding of many genomic abnormalities. By delivering massive DNA sequences at unprecedented speed and cost, NGS promises to make personalized medicine a reality in the foreseeable future. To date, library construction with clinical samples has been a challenge, primarily due to the limited quantities of sample DNA available. Our objective here was to overcome this challenge by developing NEBNext® Ultra DNA Library Prep Kit, a fast library preparation method. Specifically, we streamlined the workflow utilizing novel NEBNext reagents and adaptors, including a new DNA polymerase that has been optimized to minimize GC bias. As a result of this work, we have developed a simple method for library construction from an amount of DNA as low as 5 ng, which can be used for both intact and fragmented DNA. Moreover, the workflow is compatible with multiple NGS platforms.

77 FR 37391 - Combined Notice of Filings #2

Federal Register 2010, 2011, 2012, 2013, 2014

2012-06-21

...: Bear Swamp Power Company LLC, Brookfield Energy Marketing Inc., Brookfield Energy Marketing LP... Power, LLC, Great Lakes Hydro America LLC, Hawks Nest Hydro LLC, Longview Fibre Paper and Packaging, Inc., Rumford Falls Hydro LLC. Description: Supplement to Notice of Non-Material Change in Status of Bear Swamp...

77 FR 64501 - Combined Notice of Filings #1

Federal Register 2010, 2011, 2012, 2013, 2014

2012-10-22

.... Applicants: Calvert Cliffs Nuclear Power Plant, LLC, Nine Mile Point Nuclear Station, LLC, R.E. Ginna Nuclear Power Plant, LLC. Description: Notice of Non-Material Change in Status of Nine Mile Point Nuclear..., LLC, Shooting Star Wind Project, LLC, Safe Harbor Water Power Corporation, PECO Energy Company...

75 FR 7577 - Combined Notice of Filings # 1

Federal Register 2010, 2011, 2012, 2013, 2014

2010-02-22

... following exempt wholesale generator filings: Docket Numbers: EG10-18-000. Applicants: CER Generation, LLC. Description: Notice of Self-Certification of Exempt Wholesale Generator Status of CER Generation, LLC. Filed...Energy, Inc., Nine Mile Point Nuclear Station, LLC, CER Generation II, LLC, Handsome Lake Energy, LLC...

Kwf-Grid workflow management system for Earth science applications

NASA Astrophysics Data System (ADS)

Tran, V.; Hluchy, L.

2009-04-01

In this paper, we present workflow management tool for Earth science applications in EGEE. The workflow management tool was originally developed within K-wf Grid project for GT4 middleware and has many advanced features like semi-automatic workflow composition, user-friendly GUI for managing workflows, knowledge management. In EGEE, we are porting the workflow management tool to gLite middleware for Earth science applications K-wf Grid workflow management system was developed within "Knowledge-based Workflow System for Grid Applications" under the 6th Framework Programme. The workflow mangement system intended to - semi-automatically compose a workflow of Grid services, - execute the composed workflow application in a Grid computing environment, - monitor the performance of the Grid infrastructure and the Grid applications, - analyze the resulting monitoring information, - capture the knowledge that is contained in the information by means of intelligent agents, - and finally to reuse the joined knowledge gathered from all participating users in a collaborative way in order to efficiently construct workflows for new Grid applications. Kwf Grid workflow engines can support different types of jobs (e.g. GRAM job, web services) in a workflow. New class of gLite job has been added to the system, allows system to manage and execute gLite jobs in EGEE infrastructure. The GUI has been adapted to the requirements of EGEE users, new credential management servlet is added to portal. Porting K-wf Grid workflow management system to gLite would allow EGEE users to use the system and benefit from its avanced features. The system is primarly tested and evaluated with applications from ES clusters.

78 FR 32381 - Combined Notice of Filings #1

Federal Register 2010, 2011, 2012, 2013, 2014

2013-05-30

... that the Commission received the following electric rate filings: Docket Numbers: ER12-281-004; ER13..., Edgecombe Genco, LLC, EIF Hudson, LLC, Logan Generating Co L.P., RC Cape May Holdings, LLC, Richland-Stryker Generation LLC, Scrubgrass Generating Company, L.P., Spruance Genco, LLC, Starwood Energy Hudson Investors, L...

78 FR 4140 - Combined Notice of Filings #1

Federal Register 2010, 2011, 2012, 2013, 2014

2013-01-18

... Company and Maine Electric Power Company submit clarification to the November 30, 2012 Application for... LLC, BE Rayle LLC, Cedar Brakes I, L.L.C., Cedar Brakes II, L.L.C., Central Power & Lime LLC, Triton... that the Commission received the following electric corporate filings: Docket Numbers: EC13-49-000...

75 FR 51990 - Combined Notice of Filings #1

Federal Register 2010, 2011, 2012, 2013, 2014

2010-08-24

...-1225-009; ER09- 1321-005. Applicants: Blue Canyon Windpower II LLC, Cloud County Wind Farm, LLC, Blue Canyon Windpower LLC, Blue Canyon Windpower V LLC. Description: Supplement to Updated Market Power Anaylsis for Blue Canyon Windpower LLC, et. al. Filed Date: 08/17/2010. Accession Number: 20100817-5034...

75 FR 34170 - Plastic Omnium Automotive Exteriors, LLC, Anderson, SC; Plastic Omnium Automotive Exteriors, LLC...

Federal Register 2010, 2011, 2012, 2013, 2014

2010-06-16

... Omnium Automotive Exteriors, LLC, Anderson, SC; Plastic Omnium Automotive Exteriors, LLC, Troy, MI... the Anderson, South Carolina location of Plastic Omnium Automotive Exteriors, LLC, working out of Troy... certification to include workers in support of the Anderson, South Carolina facility working out of Troy...

75 FR 41895 - Inteva Products, LLC Adrian, Michigan; Inteva Products, LLC Troy, Michigan; Amended Certification...

Federal Register 2010, 2011, 2012, 2013, 2014

2010-07-19

... Products, LLC Adrian, Michigan; Inteva Products, LLC Troy, Michigan; Amended Certification Regarding... time period at the Troy, Michigan location of Inteva Products, LLC. The Troy, Michigan location.... Accordingly, the Department is amending the certification to include workers of the Troy, Michigan location of...

78 FR 6815 - Combined Notice of Filings #1

Federal Register 2010, 2011, 2012, 2013, 2014

2013-01-31

.... Applicants: Wildcat Wind Farm I, LLC. Description: Application For Authorization Under Section 203 Of The... Treatment Wildcat Wind Farm I, LLC. Filed Date: 1/22/13. Accession Number: 20130122-5357. Comments Due: 5 p...: High Prairie Wind Farm II, LLC, Old Trail Wind Farm, LLC, Telocaset Wind Power Partners, LLC...

78 FR 28212 - Combined Notice of Filings #1

Federal Register 2010, 2011, 2012, 2013, 2014

2013-05-14

...; ER10- 2504-004; ER12-610-004; ER13-338-002. Applicants: Catalina Solar, LLC, Oasis Power Partners, LLC.... Docket Numbers: ER13-1398-000. Applicants: Fairless Energy, LLC, Dominion Bridgeport Fuel Cell, LLC.... ET 5/23/13. Docket Numbers: ER13-1403-000. Applicants: Dominion Bridgeport Fuel Cell, LLC...

77 FR 51837 - Self-Regulatory Organizations; NYSE MKT LLC; Notice of Filing and Immediate Effectiveness of...

Federal Register 2010, 2011, 2012, 2013, 2014

2012-08-27

... Amending the NYSE Amex Options LLC Limited Liability Company Agreement To Eliminate Certain Restrictions... Options LLC (``NYSE Amex Options'') Limited Liability Company Agreement (``LLC Agreement'') to eliminate... to amend the LLC Agreement to eliminate certain restrictions relating to the qualification of...

77 FR 46428 - Combined Notice of Filings #2

Federal Register 2010, 2011, 2012, 2013, 2014

2012-08-03

... Generator Status of Spinning Spur Wind LLC. Filed Date: 7/27/12. Accession Number: 20120727-5038. Comments... II Wind Farm LLC, Fowler Ridge III Wind Farm LLC, Fowler Ridge Wind Farm LLC, Goshen Phase II, LLC... that the Commission received the following exempt wholesale generator filings: Docket Numbers: EG12-93...

77 FR 58985 - Combined Notice of Filings #1

Federal Register 2010, 2011, 2012, 2013, 2014

2012-09-25

.... Applicants: Pacific Wind Lessee, LLC, Catalina Solar, LLC. Description: Shared Transmission Facilities Agreement of Pacific Wind Lessee LLC & Catalina Solar LLC to be effective 11/15/2012. Filed Date: 9/14/12...: Pacific Gas and Electric Company. Description: E&P Agreement for SKIC Solar, LLC to be effective 9/ 17...

77 FR 12107 - RailAmerica, Inc., Palm Beach Holding, Inc., RailAmerica Transportation Corp., RailTex, Inc...

Federal Register 2010, 2011, 2012, 2013, 2014

2012-02-28

...., Palm Beach Holding, Inc., RailAmerica Transportation Corp., RailTex, Inc., Fortress Investment Group, LLC, and RR Acquisition Holding, LLC--Control Exemption--Marquette Rail, LLC AGENCY: Surface...), RailTex, Inc., Fortress Investment Group, LLC (Fortress), and RR Acquisition Holding, LLC (RR...

76 FR 22747 - RailAmerica, Inc., Palm Beach Holdings, Inc., RailAmerica Transportation Corp., RailTex, Inc...

Federal Register 2010, 2011, 2012, 2013, 2014

2011-04-22

...., Palm Beach Holdings, Inc., RailAmerica Transportation Corp., RailTex, Inc., Fortress Investment Group, LLC, and RR Acquisition Holding, LLC--Continuance in Control Exemption-- Conecuh Valley Railway, LLC...), Fortress Investment Group, LLC (Fortress), and RR Acquisition Holding, LLC (RR Acquisition) (collectively...

77 FR 69449 - Combined Notice of Filings #2

Federal Register 2010, 2011, 2012, 2013, 2014

2012-11-19

.... Applicants: Calvert Cliffs Nuclear Power Plant, LLC, Nine Mile Point Nuclear Station, LLC, R.E. Ginna Nuclear Power Plant, LLC. Description: Notice of Non-Material Change in Status of Calvert Cliffs Nuclear Power...., Constellation Power Source Generation, Inc., Cow Branch Wind Power, L.L.C., CR Clearing, LLC, Criterion Power...

77 FR 42719 - Notice of Effectiveness of Exempt Wholesale Generator or Foreign Utility Company Status

Federal Register 2010, 2011, 2012, 2013, 2014

2012-07-20

... Docket Nos. Sherbino I Wind Farm LLC EG12-43-000 Eagle Point Power Generation LLC....... EG12-53-000... Mountain Solar 2, LLC EG12-59-000 Minok Wind, LLC EG12-60-000 Senate Wind, LLC EG12-61-000 Canadian Hills Wind, LLC EG12-62-000 Moore Solar, Inc FC12-6-000 Sombra Solar, Inc FC12-7-000 Take notice that during...

78 FR 6316 - Big Blue Wind Farm, LLC, et al.; Notice of Effectiveness of Exempt Wholesale Generator Status

Federal Register 2010, 2011, 2012, 2013, 2014

2013-01-30

... Wind Farm, LLC, et al.; Notice of Effectiveness of Exempt Wholesale Generator Status Docket Nos. Big Blue Wind Farm, LLC EG13-1-000 Calpine Bosque Energy Center, LLC EG13-2-000 Homer City Generation, L.P EG13-3-000 Texas Dispatchable Wind 1, LLC EG13-4-000 Blue Creek Wind Farm LLC EG13-5-000 Take notice...

77 FR 23710 - Combined Notice of Filings #2

Federal Register 2010, 2011, 2012, 2013, 2014

2012-04-20

..., Exelon Generation Company, LLC, Exelon Energy Company, Calvert Cliffs Nuclear Power Plant LLC, CER Generation, LLC, Commonwealth Edison Company, Constellation NewEnergy, Inc., CER Generation II, LLC...

75 FR 51992 - Combined Notice of Filings #1

Federal Register 2010, 2011, 2012, 2013, 2014

2010-08-24

...: ER98-1150-011; ER07-1232-003; ER07-964-002. Applicants: Tucson Electric Company; UniSource Energy... DEPARTMENT OF ENERGY Federal Energy Regulatory Commission Combined Notice of Filings 1 August 16... Ventures Energy Corporation, BE Allegheny LLC, BE CA LLC, BE Ironwood LLC, BE KJ LLC, BE Alabama LLC, BE...

78 FR 34091 - Wausau Paper Mills, LLC; Specialty Papers Acquisition, LLC; Notice of Application for Transfer of...

Federal Register 2010, 2011, 2012, 2013, 2014

2013-06-06

... DEPARTMENT OF ENERGY Federal Energy Regulatory Commission [Project Nos. 2161-034; 2207-045] Wausau Paper Mills, LLC; Specialty Papers Acquisition, LLC; Notice of Application for Transfer of Licenses, and Soliciting Comments and Motions To Intervene On April 22, 2013, Wausau Paper Mills, LLC (transferor) and...

76 FR 13271 - DeQueen and Eastern Railroad, LLC-Corporate Family Transaction Exemption-Texas, Oklahoma...

Federal Register 2010, 2011, 2012, 2013, 2014

2011-03-10

... Eastern Railroad, LLC--Corporate Family Transaction Exemption--Texas, Oklahoma & Eastern Railroad, LLC DeQueen and Eastern Railroad, LLC (DQ&E) and Texas, Oklahoma & Eastern Railroad, LLC (TOE), have filed a... the competitive balance with carriers outside the corporate family. Under 49 U.S.C. 10502(g), the...

75 FR 11155 - Combined Notice of Filings No. 1

Federal Register 2010, 2011, 2012, 2013, 2014

2010-03-10

...; ER08-1225-009; ER09- 1321-005. Applicants: Blue Canyon Windpower II LLC, Cloud County Wind Farm, LLC, Blue Canyon Windpower LLC, Blue Canyon Windpower V LLC. Description: Updated Market Power Analysis of Blue Canyon Windpower LLC, et al. Filed Date: 02/26/2010. Accession Number: 20100226-5194. Comment Date...

76 FR 13667 - Chrysler Group LLC; Formerly Known as Chrysler LLC; Kenosha Engine Plant; Including On-Site...

Federal Register 2010, 2011, 2012, 2013, 2014

2011-03-14

...; Formerly Known as Chrysler LLC; Kenosha Engine Plant; Including On-Site Leased Workers From Caravan Knight..., K+S Services, Inc., G4S Secure Solutions, Crassociates, Inc., CES, INC., Evans Distribution Systems... of Chrysler Group, LLC, formerly known as Chrysler, LLC, Kenosha Engine Plant, Kenosha, Wisconsin...

75 FR 82426 - Big Four Terminal Railroad, LLC-Operation Exemption-RMW Ventures, LLC

Federal Register 2010, 2011, 2012, 2013, 2014

2010-12-30

... DEPARTMENT OF TRANSPORTATION Surface Transportation Board [Docket No. FD 35454] Big Four Terminal Railroad, LLC--Operation Exemption--RMW Ventures, LLC Big Four Terminal Railroad, LLC (BFTR), has filed a verified notice of exemption under 49 CFR 1150.31 to operate over 5.2 +/- miles of rail line between...

76 FR 69715 - Combined Notice of Filings #2

Federal Register 2010, 2011, 2012, 2013, 2014

2011-11-09

... Numbers: ER10-2834-001; ER10-2821-001. Applicants: Munnsville Wind Farm, LLC, Stony Creek Wind Farm, LLC. Description: Notice of Change in Status of Munnsville Wind Farm, LLC and Stony Creek Wind Farm, LLC. Filed... Numbers: ER12-328-000. Applicants: Stony Creek Wind Farm, LLC. Description: Application For Category 1...

77 FR 2287 - FFP Project 6, LLC and Northland Power Mississippi River LLC; Notice Announcing Preliminary...

Federal Register 2010, 2011, 2012, 2013, 2014

2012-01-17

... DEPARTMENT OF ENERGY Federal Energy Regulatory Commission [Project Nos. 12848-002 and 14081-000] FFP Project 6, LLC and Northland Power Mississippi River LLC; Notice Announcing Preliminary Permit... Orleans, in Orleans Parish, Louisiana. The applications were filed by FFP Project 6, LLC for Project No...

77 FR 74842 - Sunshine Act Meeting Notice

Federal Register 2010, 2011, 2012, 2013, 2014

2012-12-18

... Wind North America LLC, and Horizon Wind Energy LLC v. Bonneville Power Administration. E-3 EL11-44-002 Iberdrola Renewables, Inc., PacifiCorp, NextEra Energy Resources, LLC, Invenergy Wind North America LLC, and Horizon Wind Energy LLC v. Bonneville Power Administration. E-4 RM11-12-000 Availability of E-Tag...

76 FR 4893 - FFP Missouri 13, LLC, Grays Hydro, LLC; Notice Announcing Preliminary Permit Drawing

Federal Register 2010, 2011, 2012, 2013, 2014

2011-01-27

... DEPARTMENT OF ENERGY Federal Energy Regulatory Commission [Project No. 13763-000; Project No. 13772-000] FFP Missouri 13, LLC, Grays Hydro, LLC; Notice Announcing Preliminary Permit Drawing January... No. 13763-000, and Grays Hydro, LLC, for Project No. 13772-000. \\1\\ The Commission is open each day...

76 FR 52028 - Self-Regulatory Organizations; New York Stock Exchange LLC; Notice of Filing and Immediate...

Federal Register 2010, 2011, 2012, 2013, 2014

2011-08-19

... individual stock trading pause pursuant to the Pause Pilot on the primary listing market and those..., The NASDAQ Stock Market LLC, New York Stock Exchange LLC, NYSE Amex LLC, NYSE Arca, Inc., National...-Regulatory Organizations; New York Stock Exchange LLC; Notice of Filing and Immediate Effectiveness of...

75 FR 65515 - Supermedia LLC, Formerally Known as IDEARC Media LLC, Supermedia Information Services LLC, Client...

Federal Register 2010, 2011, 2012, 2013, 2014

2010-10-25

..., Formerally Known as IDEARC Media LLC, Supermedia Information Services LLC, Client Care Group and Publishing... September 3, 2010 and the Notice of Determination was published in the Federal Register on September 21... internal publishing support services. Workers are separately identifiable by service supplied. The negative...

78 FR 49509 - Lock+ Hydro Friends Fund XXX, LLC; FFP Project 121, LLC; Notice Announcing Preliminary Permit...

Federal Register 2010, 2011, 2012, 2013, 2014

2013-08-14

... DEPARTMENT OF ENERGY Federal Energy Regulatory Commission [ Project No. 13625-003; Project No. 14504-000] Lock+ Hydro Friends Fund XXX, LLC; FFP Project 121, LLC; Notice Announcing Preliminary Permit... and Jefferson County, Ohio. The applications were filed by Lock+ Hydro Friends Fund XXX, LLC for...

75 FR 76710 - Combined Notice of Filings No. 1

Federal Register 2010, 2011, 2012, 2013, 2014

2010-12-09

...: Astoria Energy II LLC submits tariff filing per 35: Astoria Energy II LLC. MBR E-Tariff to be effective 11... Numbers: ER11-2175-000. Applicants: SGE Energy Sourcing, LLC. Description: SGE Energy Sourcing, LLC. submits tariff filing per 35.1: Baseline Market-Based Rate Tariff Filing for SGE Energy Sourcing, LLC. to...

75 FR 20389 - Chrysler Group LLC, Formerly Known as Chrysler LLC, Warren Office Building, Including On-Site...

Federal Register 2010, 2011, 2012, 2013, 2014

2010-04-19

... certification to include workers leased from Product Action International, LLC working on-site at the Warren..., Formerly Known as Chrysler LLC, Warren Office Building, Including On-Site Leased Workers from Product Action International, LLC, Warren, Michigan; Amended Certification Regarding Eligibility To Apply for...

75 FR 56085 - Michigan Consolidated Gas Company; Enterprise Texas Pipeline LLC; Enogex LLC; Public Service...

Federal Register 2010, 2011, 2012, 2013, 2014

2010-09-15

... Storage and Transportation, L.P.; Hattiesburg Industrial Gas Sales, L.L.C.; Notice of Baseline Filings...; (Not Consolidated)] Michigan Consolidated Gas Company; Enterprise Texas Pipeline LLC; Enogex LLC... Gas Policy Act of 1978 (NGPA). Any person desiring to participate in this rate proceeding must file a...

76 FR 56428 - Southern Cross Transmission LLC; Pattern Power Marketing LLC; Notice of Filing

Federal Register 2010, 2011, 2012, 2013, 2014

2011-09-13

... DEPARTMENT OF ENERGY Federal Energy Regulatory Commission [Docket No. TX11-1-000] Southern Cross Transmission LLC; Pattern Power Marketing LLC; Notice of Filing Take notice that on September 6, 2011, pursuant... Marketing LLC (PPM) jointly filed an application requesting that the Commission direct (1) the City of...

78 FR 4842 - Linden VFT, LLC v. Brookfield Energy Marketing, LP, Cargill Power Markets, LLC; Notice of Complaint

Federal Register 2010, 2011, 2012, 2013, 2014

2013-01-23

... DEPARTMENT OF ENERGY Federal Energy Regulatory Commission [Docket No. EL13-40-000] Linden VFT, LLC v. Brookfield Energy Marketing, LP, Cargill Power Markets, LLC; Notice of Complaint Take notice that... Brookfield Energy Marketing, LP and Cargill Power Markets, LLC (Respondents) alleging that, Respondents...

75 FR 24747 - SCI, LLC/Zener-Rectifier Operations Division A Wholly Owned Subsidiary of SCI, LLC/ON...

Federal Register 2010, 2011, 2012, 2013, 2014

2010-05-05

... DEPARTMENT OF LABOR Employment and Training Administration [TA-W-70,235] SCI, LLC/Zener-Rectifier... Adjustment Assistance on October 19, 2009, applicable to workers of SCI LLC/Zener-Rectifier, Operations... Technical Resources were employed on-site at the Phoenix Arizona location of SCI LLC/Zener-Rectifier...

How smart is your BEOL? productivity improvement through intelligent automation

NASA Astrophysics Data System (ADS)

Schulz, Kristian; Egodage, Kokila; Tabbone, Gilles; Garetto, Anthony

2017-07-01

The back end of line (BEOL) workflow in the mask shop still has crucial issues throughout all standard steps which are inspection, disposition, photomask repair and verification of repair success. All involved tools are typically run by highly trained operators or engineers who setup jobs and recipes, execute tasks, analyze data and make decisions based on the results. No matter how experienced operators are and how good the systems perform, there is one aspect that always limits the productivity and effectiveness of the operation: the human aspect. Human errors can range from seemingly rather harmless slip-ups to mistakes with serious and direct economic impact including mask rejects, customer returns and line stops in the wafer fab. Even with the introduction of quality control mechanisms that help to reduce these critical but unavoidable faults, they can never be completely eliminated. Therefore the mask shop BEOL cannot run in the most efficient manner as unnecessary time and money are spent on processes that still remain labor intensive. The best way to address this issue is to automate critical segments of the workflow that are prone to human errors. In fact, manufacturing errors can occur for each BEOL step where operators intervene. These processes comprise of image evaluation, setting up tool recipes, data handling and all other tedious but required steps. With the help of smart solutions, operators can work more efficiently and dedicate their time to less mundane tasks. Smart solutions connect tools, taking over the data handling and analysis typically performed by operators and engineers. These solutions not only eliminate the human error factor in the manufacturing process but can provide benefits in terms of shorter cycle times, reduced bottlenecks and prediction of an optimized workflow. In addition such software solutions consist of building blocks that seamlessly integrate applications and allow the customers to use tailored solutions. To accommodate for the variability and complexity in mask shops today, individual workflows can be supported according to the needs of any particular manufacturing line with respect to necessary measurement and production steps. At the same time the efficiency of assets is increased by avoiding unneeded cycle time and waste of resources due to the presence of process steps that are very crucial for a given technology. In this paper we present details of which areas of the BEOL can benefit most from intelligent automation, what solutions exist and the quantification of benefits to a mask shop with full automation by the use of a back end of line model.

Fragment-based screening in tandem with phenotypic screening provides novel antiparasitic hits.

PubMed

Blaazer, Antoni R; Orrling, Kristina M; Shanmugham, Anitha; Jansen, Chimed; Maes, Louis; Edink, Ewald; Sterk, Geert Jan; Siderius, Marco; England, Paul; Bailey, David; de Esch, Iwan J P; Leurs, Rob

2015-01-01

Methods to discover biologically active small molecules include target-based and phenotypic screening approaches. One of the main difficulties in drug discovery is elucidating and exploiting the relationship between drug activity at the protein target and disease modification, a phenotypic endpoint. Fragment-based drug discovery is a target-based approach that typically involves the screening of a relatively small number of fragment-like (molecular weight <300) molecules that efficiently cover chemical space. Here, we report a fragment screening on TbrPDEB1, an essential cyclic nucleotide phosphodiesterase (PDE) from Trypanosoma brucei, and human PDE4D, an off-target, in a workflow in which fragment hits and a series of close analogs are subsequently screened for antiparasitic activity in a phenotypic panel. The phenotypic panel contained T. brucei, Trypanosoma cruzi, Leishmania infantum, and Plasmodium falciparum, the causative agents of human African trypanosomiasis (sleeping sickness), Chagas disease, leishmaniasis, and malaria, respectively, as well as MRC-5 human lung cells. This hybrid screening workflow has resulted in the discovery of various benzhydryl ethers with antiprotozoal activity and low toxicity, representing interesting starting points for further antiparasitic optimization. © 2014 Society for Laboratory Automation and Screening.

76 FR 7555 - Combined Notice of Filings #2

Federal Register 2010, 2011, 2012, 2013, 2014

2011-02-10

...Energy, Inc., CER Generation II, LLC, Safe Harbor Water Power Corporation, Handsome Lake Energy, LLC, Constellation Energy Commodities Group M, CER Generation, LLC, Criterion Power Partners, LLC. Description...

75 FR 22524 - Implantation or Injectable Dosage Form New Animal Drugs; Butorphanol

Federal Register 2010, 2011, 2012, 2013, 2014

2010-04-29

... DEPARTMENT OF HEALTH AND HUMAN SERVICES Food and Drug Administration 21 CFR Part 522 [Docket No... application (ANADA) filed by Modern Veterinary Therapeutics, LLC. The ANADA provides for use of an injectable..., 2010. FOR FURTHER INFORMATION CONTACT: John K. Harshman, Center for Veterinary Medicine (HFV-170), Food...

76 FR 27312 - Transcontinental Gas Pipe Line Company, LLC; Notice of Availability of the Environmental...

Federal Register 2010, 2011, 2012, 2013, 2014

2011-05-11

... certain natural gas pipeline facilities on its existing mainline system in Prince William, Fairfax... action significantly affecting the quality of the human environment. The National Park Service (NPS... law or special expertise with respect to resources potentially affected by the proposal and...

Developing integrated workflows for the digitisation of herbarium specimens using a modular and scalable approach.

PubMed

Haston, Elspeth; Cubey, Robert; Pullan, Martin; Atkins, Hannah; Harris, David J

2012-01-01

Digitisation programmes in many institutes frequently involve disparate and irregular funding, diverse selection criteria and scope, with different members of staff managing and operating the processes. These factors have influenced the decision at the Royal Botanic Garden Edinburgh to develop an integrated workflow for the digitisation of herbarium specimens which is modular and scalable to enable a single overall workflow to be used for all digitisation projects. This integrated workflow is comprised of three principal elements: a specimen workflow, a data workflow and an image workflow.The specimen workflow is strongly linked to curatorial processes which will impact on the prioritisation, selection and preparation of the specimens. The importance of including a conservation element within the digitisation workflow is highlighted. The data workflow includes the concept of three main categories of collection data: label data, curatorial data and supplementary data. It is shown that each category of data has its own properties which influence the timing of data capture within the workflow. Development of software has been carried out for the rapid capture of curatorial data, and optical character recognition (OCR) software is being used to increase the efficiency of capturing label data and supplementary data. The large number and size of the images has necessitated the inclusion of automated systems within the image workflow.

77 FR 12276 - Combined Notice of Filings #2

Federal Register 2010, 2011, 2012, 2013, 2014

2012-02-29

..., LLC, Bear Swamp Power Company LLC, Brookfield Energy Marketing Inc., Brookfield Energy Marketing LP...., Erie Boulevard Hydropower, L.P., Granite Reliable Power, LLC, Great Lakes Hydro America, LLC, Hawks...

77 FR 64975 - Combined Notice of Filings #1

Federal Register 2010, 2011, 2012, 2013, 2014

2012-10-24

...; ER13-111-000. Applicants: Peetz Logan Interconnect, LLC, Sagebrush, a California partnership, Sky River LLC. Description: Peetz Logan Interconnect, LLC, Sagebrush, a California partnership and Sky River LLC...

76 FR 75540 - Combined Notice of Filings #2

Federal Register 2010, 2011, 2012, 2013, 2014

2011-12-02

.... Applicants: Choctaw Gas Generation, LLC, Quantum Choctaw Power, LLC. Description: Application For... For Expedited Action of Choctaw Gas Generation, LLC and Quantum Choctaw Power, LLC. Filed Date: 11/22...

Development of an Excel-based laboratory information management system for improving workflow efficiencies in early ADME screening.

PubMed

Lu, Xinyan

2016-01-01

There is a clear requirement for enhancing laboratory information management during early absorption, distribution, metabolism and excretion (ADME) screening. The application of a commercial laboratory information management system (LIMS) is limited by complexity, insufficient flexibility, high costs and extended timelines. An improved custom in-house LIMS for ADME screening was developed using Excel. All Excel templates were generated through macros and formulae, and information flow was streamlined as much as possible. This system has been successfully applied in task generation, process control and data management, with a reduction in both labor time and human error rates. An Excel-based LIMS can provide a simple, flexible and cost/time-saving solution for improving workflow efficiencies in early ADME screening.

Dusp3 deletion in mice promotes experimental lung tumour metastasis in a macrophage dependent manner

PubMed Central

Vandereyken, Maud; Jacques, Sophie; Van Overmeire, Eva; Amand, Mathieu; Rocks, Natacha; Delierneux, Céline; Singh, Pratibha; Singh, Maneesh; Ghuysen, Camille; Wathieu, Caroline; Zurashvili, Tinatin; Sounni, Nor Eddine; Moutschen, Michel; Gilles, Christine; Oury, Cécile; Cataldo, Didier; Van Ginderachter, Jo A.

2017-01-01

Vaccinia-H1 Related (VHR) dual-specificity phosphatase, or DUSP3, plays an important role in cell cycle regulation and its expression is altered in several human cancers. In mouse model, DUSP3 deletion prevents neo-angiogenesis and b-FGF-induced microvessel outgrowth. Considering the importance of angiogenesis in metastasis formation, our study aimed to investigate the role of DUSP3 in tumour cell dissemination. Using a Lewis Lung carcinoma (LLC) experimental metastasis model, we observed that DUSP3-/- mice developed larger lung metastases than littermate controls. DUSP3-/- bone marrow transfer to lethally irradiated DUSP3+/+ mice was sufficient to transfer the phenotype to DUSP3+/+ mice, indicating that hematopoietic cells compartment was involved in the increased tumour cell dissemination to lung tissues. Interestingly, we found a higher percentage of tumour-promoting Ly6Cint macrophages in DUSP3-/- LLC-bearing lung homogenates that was at least partially due to a better recruitment of these cells. This was confirmed by 1) the presence of higher number of the Ly6Bhi macrophages in DUSP3-/- lung homogenates and by 2) the better migration of DUSP3-/- bone marrow sorted monocytes, peritoneal macrophages and bone marrow derived macrophages (BMDMs), compared to DUSP3+/+ monocytes, macrophages and BMDMs, in response to LLC-conditioned medium. Our study demonstrates that DUSP3 phosphatase plays a key role in metastatic growth through a mechanism involving the recruitment of macrophages towards LLC-bearing lungs. PMID:29020102

78 FR 25072 - Combined Notice of Filings #1

Federal Register 2010, 2011, 2012, 2013, 2014

2013-04-29

...., Central Power & Lime LLC, Triton Power Michigan LLC, Utility Contract Funding, L.L.C. Description...: ER13-1097-001. Applicants: Central Power & Lime LLC. Description: Amendment to revisions to market...

77 FR 69816 - Combined Notice of Filings #1

Federal Register 2010, 2011, 2012, 2013, 2014

2012-11-21

...: 20121113-5479. Comments Due: 5 p.m. e.t. 12/4/12. Docket Numbers: EC13-38-000. Applicants: Grand Ridge Energy LLC, Grand Ridge Energy II LLC, Grand Ridge Energy III LLC, Grand Ridge Energy IV LLC. Description... Action of Grand Ridge Energy LLC, et. al. Filed Date: 11/13/12. Accession Number: 20121113-5480. Comments...

75 FR 76041 - Chrysler Group LLC Formerly Known as Chrysler LLC Kenosha Engine Plant Including On-Site Leased...

Federal Register 2010, 2011, 2012, 2013, 2014

2010-12-07

... Formerly Known as Chrysler LLC Kenosha Engine Plant Including On-Site Leased Workers From Caravan Knight..., K+S Services, Inc., G4S Secure Solutions, Crassociates, Inc., CES, Inc., Evans Distribution Systems..., LLC, formerly known as Chrysler, LLC, Kenosha Engine Plant, Kenosha, Wisconsin (subject firm). The...

77 FR 63305 - Combined Notice of Filings #1

Federal Register 2010, 2011, 2012, 2013, 2014

2012-10-16

...: ER10-2405-002; ER10-2407-002; ER10-2425-002; ER10- 2424-002. Applicants: High Prairie Wind Farm II, LLC, Pioneer Prairie Wind Farm I, LLC, Rail Splitter Wind Farm, LLC, Lost Lakes Wind Farm LLC. Description: Supplement to Updated Market Power Analysis for the Central Region of High Prairie Wind Farm II LLC, et al...

77 FR 47670 - RG Steel Sparrows Point LLC, Formerly Known as Severstal Sparrows Point LLC, a Subsidiary of RG...

Federal Register 2010, 2011, 2012, 2013, 2014

2012-08-09

... DEPARTMENT OF LABOR Employment and Training Administration [TA-W-74,919] RG Steel Sparrows Point LLC, Formerly Known as Severstal Sparrows Point LLC, a Subsidiary of RG Steel LLC, Including All On-Site Leased Workers, Sparrows Point, MD; Amended Certification Regarding Eligibility To Apply for...

77 FR 36041 - Nevada 5, Inc. and Oakland Transportation Holdings LLC-Control Exemption-GTR Leasing LLC and US...

Federal Register 2010, 2011, 2012, 2013, 2014

2012-06-15

... DEPARTMENT OF TRANSPORTATION Surface Transportation Board [Docket No. FD 35635] Nevada 5, Inc. and Oakland Transportation Holdings LLC--Control Exemption--GTR Leasing LLC and US Rail Holdings LLC \\1\\ \\1... date of the exemption (30 days after the verified notice was filed). Applicants represent that: (1) The...

76 FR 51082 - Self-Regulatory Organizations; International Securities Exchange, LLC; Notice of Filing and...

Federal Register 2010, 2011, 2012, 2013, 2014

2011-08-17

...., International Securities Exchange LLC, The NASDAQ Stock Market LLC, New York Stock Exchange LLC, NYSE Amex LLC... include all remaining National Market System (``NMS'') stocks (``Phase III Securities'').\\9\\ The new pilot... executions, so that the rule will continue to operate in the same manner after changes to the single stock...

77 FR 11530 - Riverbank Hydro No. 2, LLC, Lock+ Hydro Friends Fund XXXVI, Qualified Hydro 21, LLC; Notice...

Federal Register 2010, 2011, 2012, 2013, 2014

2012-02-27

... DEPARTMENT OF ENERGY Federal Energy Regulatory Commission [Project Nos. 14130-000, 14137-000, 14134-000] Riverbank Hydro No. 2, LLC, Lock+ Hydro Friends Fund XXXVI, Qualified Hydro 21, LLC; Notice.... 2, LLC for Project No. 14130-000, Lock+ Hydro Friends Fund XXXVI for Project No. 14137-000, and...

77 FR 13317 - Lock+ Hydro Friends Fund XVIII, Upper Hydroelectric, LLC, FFP Project 95, LLC, Riverbank Hydro No...

Federal Register 2010, 2011, 2012, 2013, 2014

2012-03-06

... DEPARTMENT OF ENERGY Federal Energy Regulatory Commission [Project Nos. 14261-000, 14268-000, 14277-000, 14281-000] Lock+ Hydro Friends Fund XVIII, Upper Hydroelectric, LLC, FFP Project 95, LLC.... Lock+ Hydro Friends Fund XVIII: Project No. 14261-000. 2. Riverbank Hydro No. 25, LLC: Project No...

78 FR 12304 - Lock+ Hydro Friends Fund XLII, LLC; Notice of Application Ready for Environmental Analysis and...

Federal Register 2010, 2011, 2012, 2013, 2014

2013-02-22

... Friends Fund XLII, LLC; Notice of Application Ready for Environmental Analysis and Soliciting Comments...+ Hydro Friends Fund XLII, LLC. e. Name of Project: Braddock Locks and Dam Hydroelectric Project. f...\\ Hydro Friends Fund XLII, LLC, c/o Hydro Green Energy, LLC, 900 Oakmont Lane, Suite 310, Westmont, IL...

77 FR 13316 - FFP Project 91, LLC, Riverbank Hydro No. 23, LLC, Lock+ Hydro Friends Fund III; Notice Announcing...

Federal Register 2010, 2011, 2012, 2013, 2014

2012-03-06

... DEPARTMENT OF ENERGY Federal Energy Regulatory Commission [Project Nos. 14275-000, 14279-000, 14282-000] FFP Project 91, LLC, Riverbank Hydro No. 23, LLC, Lock+ Hydro Friends Fund III; Notice.... 2. Riverbank Hydro No. 23, LLC: Project No. 14279-000. 3. Lock+ Hydro Friends Fund III: Project No...

76 FR 67174 - Solia 2 Hydroelectric LLC; Riverbank Hydro No. 7 LLC; Lock Hydro Friends Fund XXXVIII Notice of...

Federal Register 2010, 2011, 2012, 2013, 2014

2011-10-31

... DEPARTMENT OF ENERGY Federal Energy Regulatory Commission [Project Nos. 14157-000; 14161-000; 14184-000] Solia 2 Hydroelectric LLC; Riverbank Hydro No. 7 LLC; Lock Hydro Friends Fund XXXVIII Notice.... 7 LLC (Riverbank) and on May 3, 2011, Lock Hydro Friends Fund XXXVIII (Lock Hydro) filed preliminary...

76 FR 67167 - Riverbank Hydro No. 1 LLC; Qualified Hydro 20 LLC; Lock Hydro Friends Fund XXXVII; Notice of...

Federal Register 2010, 2011, 2012, 2013, 2014

2011-10-31

... DEPARTMENT OF ENERGY Federal Energy Regulatory Commission [Project Nos. 14131-000; 14135-000; 14138-000] Riverbank Hydro No. 1 LLC; Qualified Hydro 20 LLC; Lock Hydro Friends Fund XXXVII; Notice of... LLC (Qualified Hydro) and Lock Hydro Friends Fund XXXVI (Lock Hydro) filed preliminary permit...

76 FR 6612 - Lock Hydro Friends Fund XLIII; FFP Missouri 14, LLC; Solia 4 Hydroelectric, LLC; Notice...

Federal Register 2010, 2011, 2012, 2013, 2014

2011-02-07

... DEPARTMENT OF ENERGY Federal Energy Regulatory Commission [Project No. 13745-000; Project No. 13758-000; Project No. 13767-000] Lock Hydro Friends Fund XLIII; FFP Missouri 14, LLC; Solia 4... Hydroelectric, LLC--Project No. 13767-000 2. FFP Missouri 14, LLC--Project No. 13758-000 3. Lock Hydro Friends...

76 FR 73615 - FFP Project 91 LLC; Riverbank Hydro No. 23 LLC; Lock Hydro Friends Fund III; Notice of Competing...

Federal Register 2010, 2011, 2012, 2013, 2014

2011-11-29

... DEPARTMENT OF ENERGY Federal Energy Regulatory Commission [Project Nos. 14275-000; 14279-000; 14282-000] FFP Project 91 LLC; Riverbank Hydro No. 23 LLC; Lock Hydro Friends Fund III; Notice of... LLC (Riverbank), and Lock Hydro Friends Fund III (Lock Hydro), filed preliminary permit applications...

76 FR 67168 - Riverbank Hydro No. 19 LLC; Lock Hydro Friends Fund XXXIV; FFP Project 59 LLC; Notice of...

Federal Register 2010, 2011, 2012, 2013, 2014

2011-10-31

... DEPARTMENT OF ENERGY Federal Energy Regulatory Commission [Project Nos. 14176-000, 14188-000, 14200-000] Riverbank Hydro No. 19 LLC; Lock Hydro Friends Fund XXXIV; FFP Project 59 LLC; Notice of..., Lock Hydro Friends Fund XXXIV (Lock Hydro), and FFP Project 59 LLC (FFP 59) filed preliminary permit...

77 FR 17121 - RailAmerica, Inc., Palm Beach Rail Holding, Inc., RailAmerica Transportation Corp., RailTex, Inc...

Federal Register 2010, 2011, 2012, 2013, 2014

2012-03-23

... Group, LLC, and RR Acquisition Holding, LLC--Control Exemption--Wellsboro & Corning Railroad, LLC Rail.... (RTC), RailTex, Inc. (RailTex), Fortress Investment Group, LLC (Fortress), and RR Acquisition Holding... acquire indirect control of the Wellsboro & Corning Railroad, LLC (W&C), a Class III rail carrier, through...

76 FR 27312 - Notice of Effectiveness of Exempt Wholesale Generator Status

Federal Register 2010, 2011, 2012, 2013, 2014

2011-05-11

... DEPARTMENT OF ENERGY Federal Energy Regulatory Commission Notice of Effectiveness of Exempt Wholesale Generator Status Docket Nos. Settlers Trail Wind Farm, LLC EG11-52-000. Avenal Park LLC EG11-53-000. Sand Drag LLC EG11-54-000. Sun City Project LLC EG11-55-000. Coyote Canyon Energy LLC EG11-56-000...

78 FR 36017 - MCM Rail Services LLC, d/b/a Baltimore Industrial Railroad-Operation Exemption-Hilco SP Rail, LLC

Federal Register 2010, 2011, 2012, 2013, 2014

2013-06-14

... DEPARTMENT OF TRANSPORTATION Surface Transportation Board [Docket No. FD 35725] MCM Rail Services LLC, d/b/a Baltimore Industrial Railroad-- Operation Exemption--Hilco SP Rail, LLC MCM Rail Services LLC, d/b/a Baltimore Industrial Railroad (MCM), a noncarrier, has filed a verified notice of exemption...

76 FR 16405 - Notice of Attendance at PJM INterconnection, L.L.C., Meetings

Federal Register 2010, 2011, 2012, 2013, 2014

2011-03-23

... INterconnection, L.L.C., Meetings The Federal Energy Regulatory Commission (Commission) hereby gives notice that members of the Commission and Commission staff may attend upcoming PJM Interconnection, L.L.C., (PJM...: Docket No. EL05-121, PJM Interconnection, L.L.C. Docket No. ER06-456, PJM Interconnection, L.L.C. Docket...

78 FR 19259 - Notice of Attendance at PJM Interconnection, L.L.C. Meetings

Federal Register 2010, 2011, 2012, 2013, 2014

2013-03-29

... Interconnection, L.L.C. Meetings The Federal Energy Regulatory Commission (Commission) hereby gives notice that members of the Commission and Commission staff may attend upcoming PJM Interconnection, L.L.C. (PJM... proceedings: Docket No. EL05-121, PJM Interconnection, L.L.C. Docket No. EL08-14, Black Oak Energy LLC, et al...

76 FR 45248 - PJM Interconnection, L.L.C., PJM Power Providers Group v. PJM Interconnection, L.L.C...

Federal Register 2010, 2011, 2012, 2013, 2014

2011-07-28

...-002; Docket No. EL11-20-001] PJM Interconnection, L.L.C., PJM Power Providers Group v. PJM Interconnection, L.L.C.; Supplemental Notice of Staff Technical Conference On June 13, 2011, the Commission issued... Resources Services, Inc., Maryland Public Service Commission, Monitoring Analytics, L.L.C., National Rural...

75 FR 70234 - Notice of Effectiveness of Exempt Wholesale Generator Status

Federal Register 2010, 2011, 2012, 2013, 2014

2010-11-17

... DEPARTMENT OF ENERGY Federal Energy Regulatory Commission [Lakefield Wind Project, LLC, EG10-57-000; Constellation Mystic Power, LLC, EG10-58-000; Pattern Gulf Wind, LLC, EG10-59-000; New Harvest Wind Project, LLC, EG10-60-000; Dry Lake Wind Power, II LLC, EG10-61-000; Learning Jupiter Wind Power...

78 FR 19699 - Combined Notice of Filings #1

Federal Register 2010, 2011, 2012, 2013, 2014

2013-04-02

.... Applicants: Alta Wind VIII, LLC, Bear Swamp Power Company LLC, Brookfield Energy Marketing, Inc., Brookfield..., Great Lakes Hydro America LLC, Hawks Nest Hydro LLC, Longview Fibre Paper and Packaging, Inc., Rumford...

75 FR 51260 - Combined Notice of Filings #1

Federal Register 2010, 2011, 2012, 2013, 2014

2010-08-19

...: CER Generation II, LLC. Description: CER Generation II, LLC submits tariff filing per 35.12: CER.... Applicants: CER Generation, LLC. Description: CER Generation, LLC submits tariff filing per 35.12: CER...

RESTFul based heterogeneous Geoprocessing workflow interoperation for Sensor Web Service

NASA Astrophysics Data System (ADS)

Yang, Chao; Chen, Nengcheng; Di, Liping

2012-10-01

Advanced sensors on board satellites offer detailed Earth observations. A workflow is one approach for designing, implementing and constructing a flexible and live link between these sensors' resources and users. It can coordinate, organize and aggregate the distributed sensor Web services to meet the requirement of a complex Earth observation scenario. A RESTFul based workflow interoperation method is proposed to integrate heterogeneous workflows into an interoperable unit. The Atom protocols are applied to describe and manage workflow resources. The XML Process Definition Language (XPDL) and Business Process Execution Language (BPEL) workflow standards are applied to structure a workflow that accesses sensor information and one that processes it separately. Then, a scenario for nitrogen dioxide (NO2) from a volcanic eruption is used to investigate the feasibility of the proposed method. The RESTFul based workflows interoperation system can describe, publish, discover, access and coordinate heterogeneous Geoprocessing workflows.

Scientific Data Management (SDM) Center for Enabling Technologies. 2007-2012

DOE Office of Scientific and Technical Information (OSTI.GOV)

Ludascher, Bertram; Altintas, Ilkay

Over the past five years, our activities have both established Kepler as a viable scientific workflow environment and demonstrated its value across multiple science applications. We have published numerous peer-reviewed papers on the technologies highlighted in this short paper and have given Kepler tutorials at SC06,SC07,SC08,and SciDAC 2007. Our outreach activities have allowed scientists to learn best practices and better utilize Kepler to address their individual workflow problems. Our contributions to advancing the state-of-the-art in scientific workflows have focused on the following areas. Progress in each of these areas is described in subsequent sections. Workflow development. The development of amore » deeper understanding of scientific workflows "in the wild" and of the requirements for support tools that allow easy construction of complex scientific workflows; Generic workflow components and templates. The development of generic actors (i.e.workflow components and processes) which can be broadly applied to scientific problems; Provenance collection and analysis. The design of a flexible provenance collection and analysis infrastructure within the workflow environment; and, Workflow reliability and fault tolerance. The improvement of the reliability and fault-tolerance of workflow environments.« less

77 FR 14513 - Combined Notice of Filings #2

Federal Register 2010, 2011, 2012, 2013, 2014

2012-03-12

..., Northern Iowa Windpower, LLC, EAM Nelson Holding, LLC, EWO Marketing, LLC, Entergy Rhode Island State Energy, L.P. Description: Supplemental information of EAM Nelson Holding, LLC, et al. Filed Date: 03/02...

77 FR 4558 - Combined Notice of Filings #1

Federal Register 2010, 2011, 2012, 2013, 2014

2012-01-30

... Numbers: EC12-62-000. Applicants: La Paloma Generating Company, LLC, Merrill Lynch Credit Products, LLC..., LLC and La Paloma Generating Company, LLC. Filed Date: 1/20/12. Accession Number: 20120120-5257...

75 FR 54610 - Combined Notice of Filings #1

Federal Register 2010, 2011, 2012, 2013, 2014

2010-09-08

..., Handsome Lake Energy, LLC, Constellation Energy Commodities Group M, CER Generation, LLC, Calvert Cliffs Nuclear Power Plant, LLC, CER Generation II, LLC. Description: Notice of change in status and Q2 2010 land...

Workflow and Proof of Concept for Non-Targeted Analysis of Environmental Samples by LC-MS/MS

EPA Science Inventory

The human exposure includes thousands of chemicals acquired through various routes of exposure such as inhalation, ingestion, dermal contact, and indirect ingestion. Rapid assessment and screening of these chemicals is a difficult challenge facing EPA in its mission to protect pu...

LATUX: An Iterative Workflow for Designing, Validating, and Deploying Learning Analytics Visualizations

ERIC Educational Resources Information Center

Martinez-Maldonado, Roberto; Pardo, Abelardo; Mirriahi, Negin; Yacef, Kalina; Kay, Judy; Clayphan, Andrew

2015-01-01

Designing, validating, and deploying learning analytics tools for instructors or students is a challenge that requires techniques and methods from different disciplines, such as software engineering, human-computer interaction, computer graphics, educational design, and psychology. Whilst each has established its own design methodologies, we now…

Ontology-Driven Discovery of Scientific Computational Entities

ERIC Educational Resources Information Center

Brazier, Pearl W.

2010-01-01

Many geoscientists use modern computational resources, such as software applications, Web services, scientific workflows and datasets that are readily available on the Internet, to support their research and many common tasks. These resources are often shared via human contact and sometimes stored in data portals; however, they are not necessarily…

77 FR 76319 - Self-Regulatory Organizations; BOX Options Exchange LLC; Order Approving Minor Rule Violation...

Federal Register 2010, 2011, 2012, 2013, 2014

2012-12-27

... Organizations; BOX Options Exchange LLC; Order Approving Minor Rule Violation Plan for BOX Options Exchange LLC December 20, 2012. On October 15, 2012, BOX Options Exchange LLC (the ``Exchange'') filed with the... Rule 19d-1(c)(2) under the Act,\\13\\ that the proposed MRVP for BOX Options Exchange LLC, File No. 4-655...

75 FR 16449 - Central Transmission, LLC v. PJM Interconnection L.L.C.; Notice of Complaint

Federal Register 2010, 2011, 2012, 2013, 2014

2010-04-01

... DEPARTMENT OF ENERGY Federal Energy Regulatory Commission [Docket No. EL10-52-000] Central Transmission, LLC v. PJM Interconnection L.L.C.; Notice of Complaint March 26, 2010. Take notice that on March... Interconnection L.L.C. (PJM) pursuant to section 206 of the Federal Power Act (FPA), alleging that Schedule 6 of...

76 FR 46793 - PJM Interconnection, L.L.C.; PJM Power Providers Group v. PJM Interconnection, L.L.C.; Notice...

Federal Register 2010, 2011, 2012, 2013, 2014

2011-08-03

...-002; Docket No. EL11-20-001] PJM Interconnection, L.L.C.; PJM Power Providers Group v. PJM Interconnection, L.L.C.; Notice Establishing Post-Technical Comment Period As indicated in the June 29, 2011... issues related to PJM Interconnection, L.L.C. (PJM)'s Minimum Offer Price Rule (MOPR) and resources...

76 FR 12954 - PPL EnergyPlus, LLC v. PJM Interconnection, L.L.C.; Notice of Complaint

Federal Register 2010, 2011, 2012, 2013, 2014

2011-03-09

... DEPARTMENT OF ENERGY Federal Energy Regulatory Commission [Docket No. EL11-25-000] PPL EnergyPlus, LLC v. PJM Interconnection, L.L.C.; Notice of Complaint Take notice that on March 2, 2011, PPL Energy... Interconnection, L.L.C. (PJM or Respondent), alleging that PJM failed to conduct its annual financial transmission...

77 FR 9225 - Notice of Effectiveness of Exempt Wholesale Generator Status

Federal Register 2010, 2011, 2012, 2013, 2014

2012-02-16

... DEPARTMENT OF ENERGY Federal Energy Regulatory Commission Notice of Effectiveness of Exempt Wholesale Generator Status Docket Nos. Fire Island Wind, LLC EG12-9-000 Kaheawa Wind Power II, LLC EG12-12-000 Broken Bow Wind, LLC EG12-13-000 Crofton Bluffs Wind, LLC EG12-14-000 NRG Texas Power LLC EG12-15...

75 FR 11530 - Crystal Lake Wind III, LLC, et al.; Notice of Effectiveness of Exempt Wholesale Generator Status

Federal Register 2010, 2011, 2012, 2013, 2014

2010-03-11

... DEPARTMENT OF ENERGY Federal Energy Regulatory Commission Crystal Lake Wind III, LLC, et al.; Notice of Effectiveness of Exempt Wholesale Generator Status March 4, 2010. Docket Nos. Crystal Lake Wind III, LLC EG10-6-000 GardenGarden Wind, LLC EG10-7-000 Star Point Wind Project LLC EG10-8-000...

77 FR 64503 - Notice of Effectiveness of Exempt Wholesale Generator Status

Federal Register 2010, 2011, 2012, 2013, 2014

2012-10-22

... DEPARTMENT OF ENERGY Federal Energy Regulatory Commission Notice of Effectiveness of Exempt Wholesale Generator Status Docket Nos. A V Solar Ranch 1, LLC EG12-83-000 Spion Kop Wind, LLC EG12-84-000... Windpower IA, LLC EG12-87-000 Los Vientos Windpower IB, LLC EG12-88-000 Mehoopany Wind Energy LLC EG12-89...

76 FR 67172 - Riverbank Hydro No. 16 LLC, Lock Hydro Friends Fund IV, FFP Project 55 LLC; Notice of Competing...

Federal Register 2010, 2011, 2012, 2013, 2014

2011-10-31

... DEPARTMENT OF ENERGY Federal Energy Regulatory Commission [Project Nos. 14167-000, 14185-000 and 14196-000] Riverbank Hydro No. 16 LLC, Lock Hydro Friends Fund IV, FFP Project 55 LLC; Notice of..., Lock Hydro Friends Fund IV (Lock Hydro), and FFP Project 55 LLC (FFP 55) filed preliminary permit...

77 FR 10741 - Lock+ Hydro Friends Fund XII, BOST2, LLC, Riverbank Hydro No. 21, LLC, FFP Project 96, LLC...

Federal Register 2010, 2011, 2012, 2013, 2014

2012-02-23

... DEPARTMENT OF ENERGY Federal Energy Regulatory Commission [Project Nos. 14260-000, 14264-000, 14267-000, 14273-000] Lock+ Hydro Friends Fund XII, BOST2, LLC, Riverbank Hydro No. 21, LLC, FFP Project... Greene and Hale counties, Alabama. The applications were filed by Lock+ Hydro Friends Fund XII for...

76 FR 67169 - Riverbank Hydro No. 10 LLC Lock Hydro Friends Fund XL FFP Project 56 LLC; Notice of Competing...

Federal Register 2010, 2011, 2012, 2013, 2014

2011-10-31

... DEPARTMENT OF ENERGY Federal Energy Regulatory Commission [Project Nos. 14164-000; 14189-000; 14198-000] Riverbank Hydro No. 10 LLC Lock Hydro Friends Fund XL FFP Project 56 LLC; Notice of Competing... Hydro Friends Fund XL (Lock Hydro), and FFP Project 56 LLC (FFP 56) filed preliminary permit...

76 FR 73614 - Lock Hydro Friends Fund XII; BOST2 LLC; Riverbank Hydro No. 21 LLC; FFP Project 96 LLC; Notice of...

Federal Register 2010, 2011, 2012, 2013, 2014

2011-11-29

... DEPARTMENT OF ENERGY Federal Energy Regulatory Commission [Project Nos. 14260-000; 14264-000; 14267-000; 14273-000] Lock Hydro Friends Fund XII; BOST2 LLC; Riverbank Hydro No. 21 LLC; FFP Project 96..., Motions To Intervene, and Competing Applications On September 1, 2011, Lock Hydro Friends Fund XII (Lock...

76 FR 6612 - Lock+ Hydro Friends Fund XL; FFP Missouri 4, LLC; Allegheny 4 Hydro, LLC; Three Rivers Hydro, LLC...

Federal Register 2010, 2011, 2012, 2013, 2014

2011-02-07

... DEPARTMENT OF ENERGY Federal Energy Regulatory Commission [Project No. 13746-000; Project No. 13750-000; Project No. 13776-000; Project No. 13782-000] Lock+ Hydro Friends Fund XL; FFP Missouri 4..., LLC--Project No. 13750-000 3. Allegheny 4 Hydro, LLC--Project No. 13776-000 4. Lock+ Hydro Friends...

76 FR 67176 - Riverbank Hydro No. 13 LLC, Lock Hydro Friends Fund XXXIV, FFP Project 55 LLC; Notice of...

Federal Register 2010, 2011, 2012, 2013, 2014

2011-10-31

... DEPARTMENT OF ENERGY Federal Energy Regulatory Commission [Project Nos. 14168-000, 14187-000 and 14199-000] Riverbank Hydro No. 13 LLC, Lock Hydro Friends Fund XXXIV, FFP Project 55 LLC; Notice of..., Lock Hydro Friends Fund XXXIV (Lock Hydro), and FFP Project 55 LLC (FFP 55) filed preliminary permit...

76 FR 67167 - Riverbank Hydro No. 6 LLC, Lock Hydro Friends Fund XLVII, FFP Project 52 LLC; Notice of Competing...

Federal Register 2010, 2011, 2012, 2013, 2014

2011-10-31

... DEPARTMENT OF ENERGY Federal Energy Regulatory Commission [Project Nos. 14172-000, 14178-000 and 14190-000] Riverbank Hydro No. 6 LLC, Lock Hydro Friends Fund XLVII, FFP Project 52 LLC; Notice of..., Lock Hydro Friends Fund XLVII (Lock Hydro), and FFP Project 52 LLC (FFP 52) filed preliminary permit...

76 FR 67172 - Riverbank Hydro No. 8 LLC Lock Hydro Friends Fund XLIV FFP Project 51 LLC; Notice of Competing...

Federal Register 2010, 2011, 2012, 2013, 2014

2011-10-31

... DEPARTMENT OF ENERGY Federal Energy Regulatory Commission [14160-000; 14179-000; 14194-000] Riverbank Hydro No. 8 LLC Lock Hydro Friends Fund XLIV FFP Project 51 LLC; Notice of Competing Preliminary... Applications On May 2, 2011, Riverbank Hydro No. 8 LLC (Riverbank), and on May 3, 2011, Lock Hydro Friends Fund...

76 FR 56873 - American Railroad Group Transportation Services, LLC d/b/a ARG Trans-Continuance in Control...

Federal Register 2010, 2011, 2012, 2013, 2014

2011-09-14

... Group Transportation Services, LLC d/b/a ARG Trans--Continuance in Control Exemption--Coos Bay Railroad Operating Company, LLC d/b/a Coos Bay Rail Link American Railroad Group Transportation Services, LLC d/b/a...) to continue in control of Coos Bay Railroad Operating Company, LLC d/b/a Coos Bay Rail Link (CBR...

77 FR 39695 - HollyFrontier Refining and Marketing LLC v. Osage Pipe Line Company, LLC; Notice of Complaint

Federal Register 2010, 2011, 2012, 2013, 2014

2012-07-05

... DEPARTMENT OF ENERGY Federal Energy Regulatory Commission [Docket No. OR12-21-000] HollyFrontier Refining and Marketing LLC v. Osage Pipe Line Company, LLC; Notice of Complaint Take notice that on June 25...; 18 CFR 343.1(a) and 343.2(c), HollyFrontier Refining and Marketing LLC (Complainant) filed a formal...

75 FR 5780 - Green Borders Geothermal, LLC, Complainant, v. Terra-Gen Dixie Valley, LLC, Respondent; Notice of...

Federal Register 2010, 2011, 2012, 2013, 2014

2010-02-04

... DEPARTMENT OF ENERGY Federal Energy Regulatory Commission [Docket No. EL10-36-000] Green Borders Geothermal, LLC, Complainant, v. Terra-Gen Dixie Valley, LLC, Respondent; Notice of Complaint January 28, 2010. Take notice that on January 25, 2010, Green Borders Geothermal, LLC (Green Borders) filed a formal complaint against Terra-Gen Dixie Valle...

77 FR 2290 - FFP Project 18, LLC, Northland Power Mississippi River LLC; Notice Announcing Preliminary Permit...

Federal Register 2010, 2011, 2012, 2013, 2014

2012-01-17

.... 14073-000] FFP Project 18, LLC, Northland Power Mississippi River LLC; Notice Announcing Preliminary... Parish, Louisiana. The applications were filed by FFP Project 18, LLC for Project No. 12857-002, and.... on the next regular business day. 18 CFR 385.2001(a)(2) (2011). On January 17, 2012, at 9 a.m...

Developing integrated workflows for the digitisation of herbarium specimens using a modular and scalable approach

PubMed Central

Haston, Elspeth; Cubey, Robert; Pullan, Martin; Atkins, Hannah; Harris, David J

2012-01-01

Abstract Digitisation programmes in many institutes frequently involve disparate and irregular funding, diverse selection criteria and scope, with different members of staff managing and operating the processes. These factors have influenced the decision at the Royal Botanic Garden Edinburgh to develop an integrated workflow for the digitisation of herbarium specimens which is modular and scalable to enable a single overall workflow to be used for all digitisation projects. This integrated workflow is comprised of three principal elements: a specimen workflow, a data workflow and an image workflow. The specimen workflow is strongly linked to curatorial processes which will impact on the prioritisation, selection and preparation of the specimens. The importance of including a conservation element within the digitisation workflow is highlighted. The data workflow includes the concept of three main categories of collection data: label data, curatorial data and supplementary data. It is shown that each category of data has its own properties which influence the timing of data capture within the workflow. Development of software has been carried out for the rapid capture of curatorial data, and optical character recognition (OCR) software is being used to increase the efficiency of capturing label data and supplementary data. The large number and size of the images has necessitated the inclusion of automated systems within the image workflow. PMID:22859881

75 FR 11916 - Chrysler Group LLC, Formerly Known as Chrysler LLC, Conner Avenue Assembly Plant, Including On...

Federal Register 2010, 2011, 2012, 2013, 2014

2010-03-12

..., CDI, Syncreon and Caravan Knight Facilities Management LLC; Detriot, MI; Amended Certification... workers from Aerotek, CDI, Syncreon and Caravan Knight Facilities Management LLC, Detroit, Michigan, who...

78 FR 9677 - Notice of Telephonic Prehearing Conference

Federal Register 2010, 2011, 2012, 2013, 2014

2013-02-11

... Matter of MAXFIELD AND OBERTON HOLDINGS, LLC; ZEN MAGNETS, LLC; and STAR NETWORKS USA, LLC; CPSC Docket... party conferencing center at (301) 504-7809. David C. Japha, Esq., Counsel for ZEN MAGNETS, LLC and STAR...

Benchmarking quantitative label-free LC-MS data processing workflows using a complex spiked proteomic standard dataset.

PubMed

Ramus, Claire; Hovasse, Agnès; Marcellin, Marlène; Hesse, Anne-Marie; Mouton-Barbosa, Emmanuelle; Bouyssié, David; Vaca, Sebastian; Carapito, Christine; Chaoui, Karima; Bruley, Christophe; Garin, Jérôme; Cianférani, Sarah; Ferro, Myriam; Van Dorssaeler, Alain; Burlet-Schiltz, Odile; Schaeffer, Christine; Couté, Yohann; Gonzalez de Peredo, Anne

2016-01-30

Proteomic workflows based on nanoLC-MS/MS data-dependent-acquisition analysis have progressed tremendously in recent years. High-resolution and fast sequencing instruments have enabled the use of label-free quantitative methods, based either on spectral counting or on MS signal analysis, which appear as an attractive way to analyze differential protein expression in complex biological samples. However, the computational processing of the data for label-free quantification still remains a challenge. Here, we used a proteomic standard composed of an equimolar mixture of 48 human proteins (Sigma UPS1) spiked at different concentrations into a background of yeast cell lysate to benchmark several label-free quantitative workflows, involving different software packages developed in recent years. This experimental design allowed to finely assess their performances in terms of sensitivity and false discovery rate, by measuring the number of true and false-positive (respectively UPS1 or yeast background proteins found as differential). The spiked standard dataset has been deposited to the ProteomeXchange repository with the identifier PXD001819 and can be used to benchmark other label-free workflows, adjust software parameter settings, improve algorithms for extraction of the quantitative metrics from raw MS data, or evaluate downstream statistical methods. Bioinformatic pipelines for label-free quantitative analysis must be objectively evaluated in their ability to detect variant proteins with good sensitivity and low false discovery rate in large-scale proteomic studies. This can be done through the use of complex spiked samples, for which the "ground truth" of variant proteins is known, allowing a statistical evaluation of the performances of the data processing workflow. We provide here such a controlled standard dataset and used it to evaluate the performances of several label-free bioinformatics tools (including MaxQuant, Skyline, MFPaQ, IRMa-hEIDI and Scaffold) in different workflows, for detection of variant proteins with different absolute expression levels and fold change values. The dataset presented here can be useful for tuning software tool parameters, and also testing new algorithms for label-free quantitative analysis, or for evaluation of downstream statistical methods. Copyright © 2015 Elsevier B.V. All rights reserved.

Building asynchronous geospatial processing workflows with web services

NASA Astrophysics Data System (ADS)

Zhao, Peisheng; Di, Liping; Yu, Genong

2012-02-01

Geoscience research and applications often involve a geospatial processing workflow. This workflow includes a sequence of operations that use a variety of tools to collect, translate, and analyze distributed heterogeneous geospatial data. Asynchronous mechanisms, by which clients initiate a request and then resume their processing without waiting for a response, are very useful for complicated workflows that take a long time to run. Geospatial contents and capabilities are increasingly becoming available online as interoperable Web services. This online availability significantly enhances the ability to use Web service chains to build distributed geospatial processing workflows. This paper focuses on how to orchestrate Web services for implementing asynchronous geospatial processing workflows. The theoretical bases for asynchronous Web services and workflows, including asynchrony patterns and message transmission, are examined to explore different asynchronous approaches to and architecture of workflow code for the support of asynchronous behavior. A sample geospatial processing workflow, issued by the Open Geospatial Consortium (OGC) Web Service, Phase 6 (OWS-6), is provided to illustrate the implementation of asynchronous geospatial processing workflows and the challenges in using Web Services Business Process Execution Language (WS-BPEL) to develop them.

Robust, Sensitive, and Automated Phosphopeptide Enrichment Optimized for Low Sample Amounts Applied to Primary Hippocampal Neurons.

PubMed

Post, Harm; Penning, Renske; Fitzpatrick, Martin A; Garrigues, Luc B; Wu, W; MacGillavry, Harold D; Hoogenraad, Casper C; Heck, Albert J R; Altelaar, A F Maarten

2017-02-03

Because of the low stoichiometry of protein phosphorylation, targeted enrichment prior to LC-MS/MS analysis is still essential. The trend in phosphoproteome analysis is shifting toward an increasing number of biological replicates per experiment, ideally starting from very low sample amounts, placing new demands on enrichment protocols to make them less labor-intensive, more sensitive, and less prone to variability. Here we assessed an automated enrichment protocol using Fe(III)-IMAC cartridges on an AssayMAP Bravo platform to meet these demands. The automated Fe(III)-IMAC-based enrichment workflow proved to be more effective when compared to a TiO 2 -based enrichment using the same platform and a manual Ti(IV)-IMAC-based enrichment workflow. As initial samples, a dilution series of both human HeLa cell and primary rat hippocampal neuron lysates was used, going down to 0.1 μg of peptide starting material. The optimized workflow proved to be efficient, sensitive, and reproducible, identifying, localizing, and quantifying thousands of phosphosites from just micrograms of starting material. To further test the automated workflow in genuine biological applications, we monitored EGF-induced signaling in hippocampal neurons, starting with only 200 000 primary cells, resulting in ∼50 μg of protein material. This revealed a comprehensive phosphoproteome, showing regulation of multiple members of the MAPK pathway and reduced phosphorylation status of two glutamate receptors involved in synaptic plasticity.

78 FR 41055 - Combined Notice of Filings #1

Federal Register 2010, 2011, 2012, 2013, 2014

2013-07-09

...; ER12-1007-003. Applicants: AP Holdings, LLC, AP Gas & Electric (IL), LLC, AP Gas & Electric (PA), LLC... Interconnection, L.L.C. Description: Ministerial Clean-Up Filing re PJM OATT Att DD 5.10 due to Overlapping...

78 FR 59662 - Combined Notice of Filings #1

Federal Register 2010, 2011, 2012, 2013, 2014

2013-09-27

... Partners II, LLC, Solar Partners VIII, LLC, Sun City Project LLC, Vienna Power LLC. Description: Notice of... between Desert Sunlight 250, 300, and Holdings to be effective 10/1/2013. Filed Date: 9/19/13. Accession...

Classical workflow nets and workflow nets with reset arcs: using Lyapunov stability for soundness verification

NASA Astrophysics Data System (ADS)

Clempner, Julio B.

2017-01-01

This paper presents a novel analytical method for soundness verification of workflow nets and reset workflow nets, using the well-known stability results of Lyapunov for Petri nets. We also prove that the soundness property is decidable for workflow nets and reset workflow nets. In addition, we provide evidence of several outcomes related with properties such as boundedness, liveness, reversibility and blocking using stability. Our approach is validated theoretically and by a numerical example related to traffic signal-control synchronisation.

76 FR 47573 - Notice of Effectiveness of Exempt Wholesale Generator Status

Federal Register 2010, 2011, 2012, 2013, 2014

2011-08-05

...-000 Sherbino II Wind Farm LLC EG11-87-000 Tanner Street Generation, LLC EG11-88-000 Inversiones E... Wholesale Generator Status Docket Nos. Bayonne Energy Center, LLC EG11-80-000 Long Island Solar Farm, LLC EG11-81-000 Evergreen Gen Lead, LLC EG11-82-000 Alta Wind IV Owner Lessor A EG11-83-000 Alta Wind IV...

77 FR 43591 - Tri-State Financial Co., LLC, (d/b/a North Country Ethanol), Red River Energy, LLC; Notice of Filing

Federal Register 2010, 2011, 2012, 2013, 2014

2012-07-25

... Financial Co., LLC, (d/b/a North Country Ethanol), Red River Energy, LLC; Notice of Filing Take notice that on July 10, 2012, Tri-State Financial Co., LLC, d/ b/a North Country Ethanol (Tri-State), and Red... the Tri- State's ethanol facility near Rosholt, in Roberts County, South Dakota (the Delivery Line...

77 FR 70478 - RG Steel Wheeling, LLC, Wheeling Office, A Division Of RG Steel, LLC, Including On-Site Leased...

Federal Register 2010, 2011, 2012, 2013, 2014

2012-11-26

... DEPARTMENT OF LABOR Employment and Training Administration [TA-W-81,880: TA-A-81,880A] RG Steel Wheeling, LLC, Wheeling Office, A Division Of RG Steel, LLC, Including On-Site Leased Workers From Pro Unlimited and Green Energy Initiatives LLC, Including Workers Whose Wages Were Reported Through Severstal Wheeling, Wheeling, WV: Mountain State...

76 FR 67177 - Riverbank Hydro No. 18 LLC, Lock Hydro Friends Fund XXXV, FFP Project 57 LLC; Notice of Competing...

Federal Register 2010, 2011, 2012, 2013, 2014

2011-10-31

...-000] Riverbank Hydro No. 18 LLC, Lock Hydro Friends Fund XXXV, FFP Project 57 LLC; Notice of Competing... Competing Applications On May 2, 2011, Riverbank Hydro No. 18 LLC (Riverbank), and on May 3, 2011, Lock... notice. Competing applications and notices of intent must meet the requirements of 18 CFR 4.36. Comments...

76 FR 14652 - Port Barre Investments, L.L.C.; Notice of Application

Federal Register 2010, 2011, 2012, 2013, 2014

2011-03-17

... Investments, L.L.C.; Notice of Application Take notice that on March 4, 2011, Port Barre Investments, L.L.C. (Bobcat) filed in Docket No. CP11-124-000 an application pursuant to section 7(c) of the Natural Gas Act... Investments, L.L.C., 126 FERC ] 61,240 (2009). \\2\\ Port Barre Investments, L.L.C., 130 FERC ] 62,272 (2010...

Biowep: a workflow enactment portal for bioinformatics applications.

PubMed

Romano, Paolo; Bartocci, Ezio; Bertolini, Guglielmo; De Paoli, Flavio; Marra, Domenico; Mauri, Giancarlo; Merelli, Emanuela; Milanesi, Luciano

2007-03-08

The huge amount of biological information, its distribution over the Internet and the heterogeneity of available software tools makes the adoption of new data integration and analysis network tools a necessity in bioinformatics. ICT standards and tools, like Web Services and Workflow Management Systems (WMS), can support the creation and deployment of such systems. Many Web Services are already available and some WMS have been proposed. They assume that researchers know which bioinformatics resources can be reached through a programmatic interface and that they are skilled in programming and building workflows. Therefore, they are not viable to the majority of unskilled researchers. A portal enabling these to take profit from new technologies is still missing. We designed biowep, a web based client application that allows for the selection and execution of a set of predefined workflows. The system is available on-line. Biowep architecture includes a Workflow Manager, a User Interface and a Workflow Executor. The task of the Workflow Manager is the creation and annotation of workflows. These can be created by using either the Taverna Workbench or BioWMS. Enactment of workflows is carried out by FreeFluo for Taverna workflows and by BioAgent/Hermes, a mobile agent-based middleware, for BioWMS ones. Main workflows' processing steps are annotated on the basis of their input and output, elaboration type and application domain by using a classification of bioinformatics data and tasks. The interface supports users authentication and profiling. Workflows can be selected on the basis of users' profiles and can be searched through their annotations. Results can be saved. We developed a web system that support the selection and execution of predefined workflows, thus simplifying access for all researchers. The implementation of Web Services allowing specialized software to interact with an exhaustive set of biomedical databases and analysis software and the creation of effective workflows can significantly improve automation of in-silico analysis. Biowep is available for interested researchers as a reference portal. They are invited to submit their workflows to the workflow repository. Biowep is further being developed in the sphere of the Laboratory of Interdisciplinary Technologies in Bioinformatics - LITBIO.

Biowep: a workflow enactment portal for bioinformatics applications

PubMed Central

Romano, Paolo; Bartocci, Ezio; Bertolini, Guglielmo; De Paoli, Flavio; Marra, Domenico; Mauri, Giancarlo; Merelli, Emanuela; Milanesi, Luciano

2007-01-01

Background The huge amount of biological information, its distribution over the Internet and the heterogeneity of available software tools makes the adoption of new data integration and analysis network tools a necessity in bioinformatics. ICT standards and tools, like Web Services and Workflow Management Systems (WMS), can support the creation and deployment of such systems. Many Web Services are already available and some WMS have been proposed. They assume that researchers know which bioinformatics resources can be reached through a programmatic interface and that they are skilled in programming and building workflows. Therefore, they are not viable to the majority of unskilled researchers. A portal enabling these to take profit from new technologies is still missing. Results We designed biowep, a web based client application that allows for the selection and execution of a set of predefined workflows. The system is available on-line. Biowep architecture includes a Workflow Manager, a User Interface and a Workflow Executor. The task of the Workflow Manager is the creation and annotation of workflows. These can be created by using either the Taverna Workbench or BioWMS. Enactment of workflows is carried out by FreeFluo for Taverna workflows and by BioAgent/Hermes, a mobile agent-based middleware, for BioWMS ones. Main workflows' processing steps are annotated on the basis of their input and output, elaboration type and application domain by using a classification of bioinformatics data and tasks. The interface supports users authentication and profiling. Workflows can be selected on the basis of users' profiles and can be searched through their annotations. Results can be saved. Conclusion We developed a web system that support the selection and execution of predefined workflows, thus simplifying access for all researchers. The implementation of Web Services allowing specialized software to interact with an exhaustive set of biomedical databases and analysis software and the creation of effective workflows can significantly improve automation of in-silico analysis. Biowep is available for interested researchers as a reference portal. They are invited to submit their workflows to the workflow repository. Biowep is further being developed in the sphere of the Laboratory of Interdisciplinary Technologies in Bioinformatics – LITBIO. PMID:17430563

Carrington Real Estate Services, LLC and Carrington Mortgage Services, LLC Information Sheet

EPA Pesticide Factsheets

Carrington Real Estate Services, LLC and Carrington Mortgage Services, LLC (the Company) is located in Anaheim, California. The settlement involves the sale of properties constructed prior to 1978, located in Bakersfield and Ridgecrest, California.

77 FR 44677 - RG Steel Sparrows Point LLC, Formerly Known as Severstal Sparrows Point LLC, a Subsidiary of RG...

Federal Register 2010, 2011, 2012, 2013, 2014

2012-07-30

... Leased Workers From Echelon Service Company, Sun Associated Industries, Inc., MPI Consultants LLC...-site leased workers from Echelon Service Company, Sun Associated Industries, Inc., MPI Consultants LLC...

Exploring the impact of an automated prescription-filling device on community pharmacy technician workflow.

PubMed

Walsh, Kristin E; Chui, Michelle Anne; Kieser, Mara A; Williams, Staci M; Sutter, Susan L; Sutter, John G

2011-01-01

To explore community pharmacy technician workflow change after implementation of an automated robotic prescription-filling device. At an independent community pharmacy in rural Mayville, WI, pharmacy technicians were observed before and 3 months after installation of an automated robotic prescription-filling device. The main outcome measures were sequences and timing of technician workflow steps, workflow interruptions, automation surprises, and workarounds. Of the 77 and 80 observations made before and 3 months after robot installation, respectively, 17 different workflow sequences were observed before installation and 38 after installation. Average prescription filling time was reduced by 40 seconds per prescription with use of the robot. Workflow interruptions per observation increased from 1.49 to 1.79 (P = 0.11), and workarounds increased from 10% to 36% after robot use. Although automated prescription-filling devices can increase efficiency, workflow interruptions and workarounds may negate that efficiency. Assessing changes in workflow and sequencing of tasks that may result from the use of automation can help uncover opportunities for workflow policy and procedure redesign.

Generic worklist handler for workflow-enabled products

NASA Astrophysics Data System (ADS)

Schmidt, Joachim; Meetz, Kirsten; Wendler, Thomas

1999-07-01

Workflow management (WfM) is an emerging field of medical information technology. It appears as a promising key technology to model, optimize and automate processes, for the sake of improved efficiency, reduced costs and improved patient care. The Application of WfM concepts requires the standardization of architectures and interfaces. A component of central interest proposed in this report is a generic work list handler: A standardized interface between a workflow enactment service and application system. Application systems with embedded work list handlers will be called 'Workflow Enabled Application Systems'. In this paper we discus functional requirements of work list handlers, as well as their integration into workflow architectures and interfaces. To lay the foundation for this specification, basic workflow terminology, the fundamentals of workflow management and - later in the paper - the available standards as defined by the Workflow Management Coalition are briefly reviewed.

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Phosphorylation of Stats at Ser727 in renal proximal tubular epithelial cells exposed to cadmium.

PubMed

Nakagawa, Junko; Nishitai, Gen; Inageda, Kiyoshi; Matsuoka, Masato

2007-11-01

The effects of cadmium exposure on serine phosphorylation of signal transducers and activators of transcription (Stats) and an upstream kinase were examined in renal proximal tubular cells. In porcine LLC-PK1 cells treated with cadmium, Stat1 and Stat3 proteins were phosphorylated at Ser727 without changing total Stat protein levels. While phosphorylated forms of the members of mitogen-activated protein kinases (MAPKs) increased in response to cadmium exposure, treatment with a p38 inhibitor, SB203580 reduced Ser727 phosphorylation of Stat1 and Stat3 markedly in LLC-PK1 cells. The expression of human matrix metalloproteinase-3 (MMP-3), a Stats-inducible gene, was found to be up-regulated in human HK-2 cells exposed to cadmium, and suppressed by preincubation with SB203580. These results suggest that cadmium might induce the phosphorylation of Stat1 and Stat3 at Ser727 via the p38 pathway at least in part, and modulate gene expression in these proximal tubular cells. Copyright © 2007 Elsevier B.V. All rights reserved.

76 FR 44319 - Combined Notice of Filings #1

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Workflow as a Service in the Cloud: Architecture and Scheduling Algorithms.

PubMed

Wang, Jianwu; Korambath, Prakashan; Altintas, Ilkay; Davis, Jim; Crawl, Daniel

2014-01-01

With more and more workflow systems adopting cloud as their execution environment, it becomes increasingly challenging on how to efficiently manage various workflows, virtual machines (VMs) and workflow execution on VM instances. To make the system scalable and easy-to-extend, we design a Workflow as a Service (WFaaS) architecture with independent services. A core part of the architecture is how to efficiently respond continuous workflow requests from users and schedule their executions in the cloud. Based on different targets, we propose four heuristic workflow scheduling algorithms for the WFaaS architecture, and analyze the differences and best usages of the algorithms in terms of performance, cost and the price/performance ratio via experimental studies.

Tigres Workflow Library: Supporting Scientific Pipelines on HPC Systems

DOE PAGES

Hendrix, Valerie; Fox, James; Ghoshal, Devarshi; ...

2016-07-21

The growth in scientific data volumes has resulted in the need for new tools that enable users to operate on and analyze data on large-scale resources. In the last decade, a number of scientific workflow tools have emerged. These tools often target distributed environments, and often need expert help to compose and execute the workflows. Data-intensive workflows are often ad-hoc, they involve an iterative development process that includes users composing and testing their workflows on desktops, and scaling up to larger systems. In this paper, we present the design and implementation of Tigres, a workflow library that supports the iterativemore » workflow development cycle of data-intensive workflows. Tigres provides an application programming interface to a set of programming templates i.e., sequence, parallel, split, merge, that can be used to compose and execute computational and data pipelines. We discuss the results of our evaluation of scientific and synthetic workflows showing Tigres performs with minimal template overheads (mean of 13 seconds over all experiments). We also discuss various factors (e.g., I/O performance, execution mechanisms) that affect the performance of scientific workflows on HPC systems.« less

Tigres Workflow Library: Supporting Scientific Pipelines on HPC Systems

DOE Office of Scientific and Technical Information (OSTI.GOV)

Hendrix, Valerie; Fox, James; Ghoshal, Devarshi

The growth in scientific data volumes has resulted in the need for new tools that enable users to operate on and analyze data on large-scale resources. In the last decade, a number of scientific workflow tools have emerged. These tools often target distributed environments, and often need expert help to compose and execute the workflows. Data-intensive workflows are often ad-hoc, they involve an iterative development process that includes users composing and testing their workflows on desktops, and scaling up to larger systems. In this paper, we present the design and implementation of Tigres, a workflow library that supports the iterativemore » workflow development cycle of data-intensive workflows. Tigres provides an application programming interface to a set of programming templates i.e., sequence, parallel, split, merge, that can be used to compose and execute computational and data pipelines. We discuss the results of our evaluation of scientific and synthetic workflows showing Tigres performs with minimal template overheads (mean of 13 seconds over all experiments). We also discuss various factors (e.g., I/O performance, execution mechanisms) that affect the performance of scientific workflows on HPC systems.« less

Standardizing clinical trials workflow representation in UML for international site comparison.

PubMed

de Carvalho, Elias Cesar Araujo; Jayanti, Madhav Kishore; Batilana, Adelia Portero; Kozan, Andreia M O; Rodrigues, Maria J; Shah, Jatin; Loures, Marco R; Patil, Sunita; Payne, Philip; Pietrobon, Ricardo

2010-11-09

With the globalization of clinical trials, a growing emphasis has been placed on the standardization of the workflow in order to ensure the reproducibility and reliability of the overall trial. Despite the importance of workflow evaluation, to our knowledge no previous studies have attempted to adapt existing modeling languages to standardize the representation of clinical trials. Unified Modeling Language (UML) is a computational language that can be used to model operational workflow, and a UML profile can be developed to standardize UML models within a given domain. This paper's objective is to develop a UML profile to extend the UML Activity Diagram schema into the clinical trials domain, defining a standard representation for clinical trial workflow diagrams in UML. Two Brazilian clinical trial sites in rheumatology and oncology were examined to model their workflow and collect time-motion data. UML modeling was conducted in Eclipse, and a UML profile was developed to incorporate information used in discrete event simulation software. Ethnographic observation revealed bottlenecks in workflow: these included tasks requiring full commitment of CRCs, transferring notes from paper to computers, deviations from standard operating procedures, and conflicts between different IT systems. Time-motion analysis revealed that nurses' activities took up the most time in the workflow and contained a high frequency of shorter duration activities. Administrative assistants performed more activities near the beginning and end of the workflow. Overall, clinical trial tasks had a greater frequency than clinic routines or other general activities. This paper describes a method for modeling clinical trial workflow in UML and standardizing these workflow diagrams through a UML profile. In the increasingly global environment of clinical trials, the standardization of workflow modeling is a necessary precursor to conducting a comparative analysis of international clinical trials workflows.

Standardizing Clinical Trials Workflow Representation in UML for International Site Comparison

PubMed Central

de Carvalho, Elias Cesar Araujo; Jayanti, Madhav Kishore; Batilana, Adelia Portero; Kozan, Andreia M. O.; Rodrigues, Maria J.; Shah, Jatin; Loures, Marco R.; Patil, Sunita; Payne, Philip; Pietrobon, Ricardo

2010-01-01

Background With the globalization of clinical trials, a growing emphasis has been placed on the standardization of the workflow in order to ensure the reproducibility and reliability of the overall trial. Despite the importance of workflow evaluation, to our knowledge no previous studies have attempted to adapt existing modeling languages to standardize the representation of clinical trials. Unified Modeling Language (UML) is a computational language that can be used to model operational workflow, and a UML profile can be developed to standardize UML models within a given domain. This paper's objective is to develop a UML profile to extend the UML Activity Diagram schema into the clinical trials domain, defining a standard representation for clinical trial workflow diagrams in UML. Methods Two Brazilian clinical trial sites in rheumatology and oncology were examined to model their workflow and collect time-motion data. UML modeling was conducted in Eclipse, and a UML profile was developed to incorporate information used in discrete event simulation software. Results Ethnographic observation revealed bottlenecks in workflow: these included tasks requiring full commitment of CRCs, transferring notes from paper to computers, deviations from standard operating procedures, and conflicts between different IT systems. Time-motion analysis revealed that nurses' activities took up the most time in the workflow and contained a high frequency of shorter duration activities. Administrative assistants performed more activities near the beginning and end of the workflow. Overall, clinical trial tasks had a greater frequency than clinic routines or other general activities. Conclusions This paper describes a method for modeling clinical trial workflow in UML and standardizing these workflow diagrams through a UML profile. In the increasingly global environment of clinical trials, the standardization of workflow modeling is a necessary precursor to conducting a comparative analysis of international clinical trials workflows. PMID:21085484

78 FR 70933 - Combined Notice of Filings #1

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2013-11-27

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76 FR 62795 - Combined Notice of Filings #1

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... DEPARTMENT OF ENERGY Federal Energy Regulatory Commission Combined Notice of Filings 1 Take notice...-000. Applicants: Pioneer Trail Wind Farm, LLC. Description: Pioneer Trail Wind Farm, LLC, Notice of...: ER11-4695-000. Applicants: Hafslund Energy Trading LLC. Description: Hafslund Energy Trading LLC...

Age- and Activity-Related Differences in the Abundance of Myosin Essential and Regulatory Light Chains in Human Muscle

PubMed Central

Cobley, James N.; Ab. Malik, Zulezwan; Morton, James P.; Close, Graeme L.; Edwards, Ben J.; Burniston, Jatin G.

2016-01-01

Traditional methods for phenotyping skeletal muscle (e.g., immunohistochemistry) are labor-intensive and ill-suited to multixplex analysis, i.e., assays must be performed in a series. Addressing these concerns represents a largely unmet research need but more comprehensive parallel analysis of myofibrillar proteins could advance knowledge regarding age- and activity-dependent changes in human muscle. We report a label-free, semi-automated and time efficient LC-MS proteomic workflow for phenotyping the myofibrillar proteome. Application of this workflow in old and young as well as trained and untrained human skeletal muscle yielded several novel observations that were subsequently verified by multiple reaction monitoring (MRM). We report novel data demonstrating that human ageing is associated with lesser myosin light chain 1 content and greater myosin light chain 3 content, consistent with an age-related reduction in type II muscle fibers. We also disambiguate conflicting data regarding myosin regulatory light chain, revealing that age-related changes in this protein more closely reflect physical activity status than ageing per se. This finding reinforces the need to control for physical activity levels when investigating the natural process of ageing. Taken together, our data confirm and extend knowledge regarding age- and activity-related phenotypes. In addition, the MRM transitions described here provide a methodological platform that can be fine-tuned to suite multiple research needs and thus advance myofibrillar phenotyping. PMID:28248225

Rapid resistome mapping using nanopore sequencing

PubMed Central

Imamovic, Lejla; Hashim Ellabaan, Mostafa M.; van Schaik, Willem; Koza, Anna

2017-01-01

Abstract The emergence of antibiotic resistance in human pathogens has become a major threat to modern medicine. The outcome of antibiotic treatment can be affected by the composition of the gut. Accordingly, knowledge of the gut resistome composition could enable more effective and individualized treatment of bacterial infections. Yet, rapid workflows for resistome characterization are lacking. To address this challenge we developed the poreFUME workflow that deploys functional metagenomic selections and nanopore sequencing to resistome mapping. We demonstrate the approach by functionally characterizing the gut resistome of an ICU (intensive care unit) patient. The accuracy of the poreFUME pipeline is with >97% sufficient for the annotation of antibiotic resistance genes. The poreFUME pipeline provides a promising approach for efficient resistome profiling that could inform antibiotic treatment decisions in the future. PMID:28062856

Cytotoxic constituents from Brazilian red propolis and their structure-activity relationship.

PubMed

Li, Feng; Awale, Suresh; Tezuka, Yasuhiro; Kadota, Shigetoshi

2008-05-15

Several classes of flavonoids [flavanoids (1-10), flavonol (11), isoflavones (12-18), isoflavanones (19-22), isoflavans (23-26), chalcones (27-30), auronol (31), pterocarpans (32-37), 2-arylbenzofuran (38), and neoflavonoid (39)] and lignans (40-42) isolated from the MeOH extract of Brazilian red propolis were investigated for their cytotoxic activity against a panel of six different cancer cell lines including murine colon 26-L5 carcinoma, murine B16-BL6 melanoma, murine Lewis lung carcinoma, human lung A549 adenocarcinoma, human cervix HeLa adenocarcinoma, and human HT-1080 fibrosarcoma cell lines. Based on the observed results, structure-activity relationships were discussed. Among the tested compounds, 7-hydroxy-6-methoxyflavanone (3) exhibited the most potent activity against B16-BL6 (IC(50), 6.66microM), LLC (IC(50), 9.29microM), A549 (IC(50), 8.63microM), and HT-1080 (IC(50), 7.94microM) cancer cell lines, and mucronulatol (26) against LLC (IC(50), 8.38microM) and A549 (IC(50), 9.9microM) cancer cell lines. These activity data were comparable to those of the clinically used anticancer drugs, 5-fluorouracil and doxorubicin, against the tested cell lines, suggesting that 3 and 26 are the good candidates for future anticancer drug development.

Scan-layered reconstructions: A pilot study of a nondestructive dental histoanatomical analysis method and digital workflow to create restorations driven by natural dentin and enamel morphology.

PubMed

Malta Barbosa, João; Tovar, Nick; A Tuesta, Pablo; Hirata, Ronaldo; Guimarães, Nuno; Romanini, José C; Moghadam, Marjan; Coelho, Paulo G; Jahangiri, Leila

2017-07-08

This work aims to present a pilot study of a non-destructive dental histo-anatomical analysis technique as well as to push the boundaries of the presently available restorative workflows for the fabrication of highly customized ceramic restorations. An extracted human maxillary central incisor was subject to a micro computed tomography scan and the acquired data was transferred into a workstation, reconstructed, segmented, evaluated and later imported into a Computer-Aided Design/Computer-Aided Manufacturing software for the fabrication of a ceramic resin-bonded prosthesis. The obtained prosthesis presented an encouraging optical behavior and was used clinically as final restoration. The digitally layered restorative replication of natural tooth morphology presents today as a clear possibility. New clinical and laboratory-fabricated, biologically inspired digital restorative protocols are to be expected in the near future. The digitally layered restorative replication of natural tooth morphology presents today as a clear possibility. This pilot study may represent a stimulus for future research and applications of digital imaging as well as digital restorative workflows in service of esthetic dentistry. © 2017 Wiley Periodicals, Inc.

75 FR 13757 - Combined Notice of Filings #1

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2010-03-23

..., Inc.; UniSource Energy Development Company. Description: Tucson Electric Power Co. submits a... DEPARTMENT OF ENERGY Federal Energy Regulatory Commission Combined Notice of Filings 1 March 16....P. Morgan Ventures Energy Corporation, BE Allegheny LLC, BE CA LLC, BE Ironwood LLC,BE KJ LLC, BE...

76 FR 41829 - J.P. Morgan Securities LLC, et al.; Notice of Application and Temporary Order

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A robust ambient temperature collection and stabilization strategy: Enabling worldwide functional studies of the human microbiome

PubMed Central

Anderson, Ericka L.; Li, Weizhong; Klitgord, Niels; Highlander, Sarah K.; Dayrit, Mark; Seguritan, Victor; Yooseph, Shibu; Biggs, William; Venter, J. Craig; Nelson, Karen E.; Jones, Marcus B.

2016-01-01

As reports on possible associations between microbes and the host increase in number, more meaningful interpretations of this information require an ability to compare data sets across studies. This is dependent upon standardization of workflows to ensure comparability both within and between studies. Here we propose the standard use of an alternate collection and stabilization method that would facilitate such comparisons. The DNA Genotek OMNIgene∙Gut Stool Microbiome Kit was compared to the currently accepted community standard of freezing to store human stool samples prior to whole genome sequencing (WGS) for microbiome studies. This stabilization and collection device allows for ambient temperature storage, automation, and ease of shipping/transfer of samples. The device permitted the same data reproducibility as with frozen samples, and yielded higher recovery of nucleic acids. Collection and stabilization of stool microbiome samples with the DNA Genotek collection device, combined with our extraction and WGS, provides a robust, reproducible workflow that enables standardized global collection, storage, and analysis of stool for microbiome studies. PMID:27558918

Workflow as a Service in the Cloud: Architecture and Scheduling Algorithms

PubMed Central

Wang, Jianwu; Korambath, Prakashan; Altintas, Ilkay; Davis, Jim; Crawl, Daniel

2017-01-01

With more and more workflow systems adopting cloud as their execution environment, it becomes increasingly challenging on how to efficiently manage various workflows, virtual machines (VMs) and workflow execution on VM instances. To make the system scalable and easy-to-extend, we design a Workflow as a Service (WFaaS) architecture with independent services. A core part of the architecture is how to efficiently respond continuous workflow requests from users and schedule their executions in the cloud. Based on different targets, we propose four heuristic workflow scheduling algorithms for the WFaaS architecture, and analyze the differences and best usages of the algorithms in terms of performance, cost and the price/performance ratio via experimental studies. PMID:29399237

Scientific Data Management (SDM) Center for Enabling Technologies. Final Report, 2007-2012

DOE Office of Scientific and Technical Information (OSTI.GOV)

Ludascher, Bertram; Altintas, Ilkay

Our contributions to advancing the State of the Art in scientific workflows have focused on the following areas: Workflow development; Generic workflow components and templates; Provenance collection and analysis; and, Workflow reliability and fault tolerance.

Lipid-laden cells differentially distributed in the aging brain are functionally active and correspond to distinct phenotypes

PubMed Central

Shimabukuro, Marilia Kimie; Langhi, Larissa Gutman Paranhos; Cordeiro, Ingrid; Brito, José M.; Batista, Claudia Maria de Castro; Mattson, Mark P.; de Mello Coelho, Valeria

2016-01-01

We characterized cerebral Oil Red O-positive lipid-laden cells (LLC) of aging mice evaluating their distribution, morphology, density, functional activities and inflammatory phenotype. We identified LLC in meningeal, cortical and neurogenic brain regions. The density of cerebral LLC increased with age. LLC presenting small lipid droplets were visualized adjacent to blood vessels or deeper in the brain cortical and striatal parenchyma of aging mice. LLC with larger droplets were asymmetrically distributed in the cerebral ventricle walls, mainly located in the lateral wall. We also found that LLC in the subventricular region co-expressed beclin-1 or LC3, markers for autophagosome or autophagolysosome formation, and perilipin (PLIN), a lipid droplet-associated protein, suggesting lipophagic activity. Some cerebral LLC exhibited β galactosidase activity indicating a senescence phenotype. Moreover, we detected production of the pro-inflammatory cytokine TNF-α in cortical PLIN+ LLC. Some cortical NeuN+ neurons, GFAP+ glia limitans astrocytes, Iba-1+ microglia and S100β+ ependymal cells expressed PLIN in the aging brain. Our findings suggest that cerebral LLC exhibit distinct cellular phenotypes and may participate in the age-associated neuroinflammatory processes. PMID:27029648

Lipid-laden cells differentially distributed in the aging brain are functionally active and correspond to distinct phenotypes.

PubMed

Shimabukuro, Marilia Kimie; Langhi, Larissa Gutman Paranhos; Cordeiro, Ingrid; Brito, José M; Batista, Claudia Maria de Castro; Mattson, Mark P; Mello Coelho, Valeria de

2016-03-31

We characterized cerebral Oil Red O-positive lipid-laden cells (LLC) of aging mice evaluating their distribution, morphology, density, functional activities and inflammatory phenotype. We identified LLC in meningeal, cortical and neurogenic brain regions. The density of cerebral LLC increased with age. LLC presenting small lipid droplets were visualized adjacent to blood vessels or deeper in the brain cortical and striatal parenchyma of aging mice. LLC with larger droplets were asymmetrically distributed in the cerebral ventricle walls, mainly located in the lateral wall. We also found that LLC in the subventricular region co-expressed beclin-1 or LC3, markers for autophagosome or autophagolysosome formation, and perilipin (PLIN), a lipid droplet-associated protein, suggesting lipophagic activity. Some cerebral LLC exhibited β galactosidase activity indicating a senescence phenotype. Moreover, we detected production of the pro-inflammatory cytokine TNF-α in cortical PLIN(+) LLC. Some cortical NeuN(+) neurons, GFAP(+) glia limitans astrocytes, Iba-1(+) microglia and S100β(+) ependymal cells expressed PLIN in the aging brain. Our findings suggest that cerebral LLC exhibit distinct cellular phenotypes and may participate in the age-associated neuroinflammatory processes.

Exploring the impact of an automated prescription-filling device on community pharmacy technician workflow

PubMed Central

Walsh, Kristin E.; Chui, Michelle Anne; Kieser, Mara A.; Williams, Staci M.; Sutter, Susan L.; Sutter, John G.

2012-01-01

Objective To explore community pharmacy technician workflow change after implementation of an automated robotic prescription-filling device. Methods At an independent community pharmacy in rural Mayville, WI, pharmacy technicians were observed before and 3 months after installation of an automated robotic prescription-filling device. The main outcome measures were sequences and timing of technician workflow steps, workflow interruptions, automation surprises, and workarounds. Results Of the 77 and 80 observations made before and 3 months after robot installation, respectively, 17 different workflow sequences were observed before installation and 38 after installation. Average prescription filling time was reduced by 40 seconds per prescription with use of the robot. Workflow interruptions per observation increased from 1.49 to 1.79 (P = 0.11), and workarounds increased from 10% to 36% after robot use. Conclusion Although automated prescription-filling devices can increase efficiency, workflow interruptions and workarounds may negate that efficiency. Assessing changes in workflow and sequencing of tasks that may result from the use of automation can help uncover opportunities for workflow policy and procedure redesign. PMID:21896459

75 FR 24687 - Combined Notice of Filings #1

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77 FR 74245 - ING Investments, LLC, et al.; Notice of Application

Federal Register 2010, 2011, 2012, 2013, 2014

2012-12-13

... Investments, LLC (``IIL''), Directed Services LLC (``DSL'') and ING Investment Management Co. LLC (``IIM..., at (202) 551-6821 (Division of Investment Management, Office of Investment Company Regulation..., N.V. 2. Each Registrant is registered under the Act as an open-end management investment company...

75 FR 56641 - Self-Regulatory Organizations; Financial Industry Regulatory Authority, Inc.; Order Granting...

Federal Register 2010, 2011, 2012, 2013, 2014

2010-09-16

... Stock Exchange, Inc. (``CHX''), EDGA Exchange, Inc. (``EDGA''), EDGX Exchange, Inc. (``EDGX''), International Securities Exchange LLC (``ISE''), The NASDAQ Stock Market LLC (``Nasdaq''), National Stock Exchange, Inc. (``NSX''), New York Stock Exchange LLC (``NYSE''), NYSE Amex LLC (``NYSE Amex''), NYSE Arca...

77 FR 24481 - Combined Notice of Filings #1

Federal Register 2010, 2011, 2012, 2013, 2014

2012-04-24

... Power North, LLC. Description: Silver State Solar Power North LLC Baseline Tariff Amendment to be... Market Power Update of CPV Batesville, LLC. Filed Date: 3/21/12. Accession Number: 20120321-5067...: ER12-799-000. Applicants: Nevada Solar One, LLC. Description: Supplement to Request for Determination...

78 FR 34366 - Combined Notice of Filings #1

Federal Register 2010, 2011, 2012, 2013, 2014

2013-06-07

... Solar, LLC. Description: Application and Initial Baseline Tariff Filing to be effective 8/1/2013. Filed... DEPARTMENT OF ENERGY Federal Energy Regulatory Commission Combined Notice of Filings 1 Take notice.... Applicants: Beech Ridge Energy LLC, Bishop Hill Energy LLC, Bishop Hill Energy III LLC, Bishop Hill...

75 FR 11162 - Combined Notice of Filings #1

Federal Register 2010, 2011, 2012, 2013, 2014

2010-03-10

...; Wildorado Wind, LLC. Description: San Juan Mesa Wind Project, LLC et al. submits the Updated Market Power... Power Marketing, LLC; High Majestic Wind Energy Center, LLC. Description: NextEra Companies submits.... Description: Golden Spread Electric Cooperative, Inc et al. submits an Updated Market Power Analysis. Filed...

78 FR 79428 - Combined Notice of Filings #1

Federal Register 2010, 2011, 2012, 2013, 2014

2013-12-30

...-000. Applicants: Pleasant Valley Wind, LLC. Description: Notice of Self-Certification of EG of Pleasant Valley Wind, LLC. Filed Date: 12/17/13. Accession Number: 20131217-5279. Comments Due: 5 p.m. ET 1...-001. Applicants: Canandaigua Power Partners, LLC. Description: Canandaigua Power Partners, LLC submits...

77 FR 66828 - Combined Notice of Filings #2

Federal Register 2010, 2011, 2012, 2013, 2014

2012-11-07

... Industrial Power Services (NY), LLC, EDF Industrial Power Services (IL), LLC, EDF Industrial Power Services... reports: Docket Numbers: LA12-3-000. Applicants: Carolina Power & Light Company, Cimarron Windpower II... Duke Energy Ohio, Inc. Duke Energy Retail Sales, LLC, Duke Energy Washington II, LLC, Florida Power...

78 FR 77493 - Notice of Lodging of Proposed Consent Decree Under the Resource Conservation and Recovery Act...

Federal Register 2010, 2011, 2012, 2013, 2014

2013-12-23

... Metallurgical, LLC and TDY Industries, LLC, 6:13-cv-02188-TC. This is a civil action resulting from the improper... should refer to United States v. Oregon Metallurgical, LLC and TDY Industries, LLC, 6:13-cv-02188-TC...

77 FR 41976 - Combined Notice of Filings #1

Federal Register 2010, 2011, 2012, 2013, 2014

2012-07-17

...-2200-002; ER12-1716-001. Applicants: Noble Americas Gas & Power Corp., Noble Americas Energy Solutions LLC, Your Energy Holdings, LLC. Description: Notice of Change in Status of Noble Americas Gas & Power...-004. Applicants: EDF Trading North America, LLC, EDF Industrial Power Services (IL), LLC, EDF...

75 FR 60138 - Chrysler Financial Services Americas, LLC, a Subsidiary of Finco Intermediate Holding Co., LLC...

Federal Register 2010, 2011, 2012, 2013, 2014

2010-09-29

... DEPARTMENT OF LABOR Employment and Training Administration [TA-W-73,458] Chrysler Financial Services Americas, LLC, a Subsidiary of Finco Intermediate Holding Co., LLC, Troy Customer Contact Center, Troy, Michigan; Notice of Affirmative Determination Regarding Application for Reconsideration By...

75 FR 53699 - Granting of Request for Early Termination of the Waiting Period Under the Premerger Notification...

Federal Register 2010, 2011, 2012, 2013, 2014

2010-09-01

... International, Inc. 20100910 G Galaxy PEF Holding LLC. G Otera US Holding Inc. G CW Financial Services LLC. 20100911 G Galaxy CF UST Investment Holdings LLC. G Otera US Holding Inc. G CW Financial Services LLC. 30...

77 FR 68761 - Combined Notice of Filings #1

Federal Register 2010, 2011, 2012, 2013, 2014

2012-11-16

... Energy Marketing LLC, Champion Energy Services, LLC, Champion Energy, LLC. Description: Supplement to Updated Market Power Analysis for the Central Region of Champion Energy Marketing LLC, et al. Filed Date... and Related Values for the 2016/2017 Capability Year. Filed Date: 11/6/12. Accession Number: 20121106...

Lip line changes in Class III facial asymmetry patients after orthodontic camouflage treatment, one-jaw surgery, and two-jaw surgery: A preliminary study.

PubMed

Lee, Gung-Chol; Yoo, Jo-Kwang; Kim, Seong-Hun; Moon, Cheol-Hyun

2017-03-01

To evaluate the effects of orthodontic camouflage treatment (OCT), one-jaw surgery, and two-jaw surgery on the correction of lip line cant (LLC) and to examine factors affecting the correction of LLC in Class III craniofacial asymmetry patients. A sample of 30 Class III craniofacial asymmetry patients was divided into OCT (n = 10), one-jaw surgery (n = 10), and two-jaw surgery (n = 10) groups such that the pretreatment LLC was similar in each group. Pretreatment and posttreatment cone-beam computed tomography scans were used to measure dental and skeletal parameters and LLC. Pretreatment and posttreatment measurements were compared within groups and between groups. Pearson's correlation tests and multiple regression analyses were performed to investigate factors affecting the amount and rate of LLC correction. The average LLC correction was 1.00° in the one-jaw surgery group, and in the two-jaw surgery group, it was 1.71°. In the OCT group it was -0.04°, which differed statistically significantly from the LLC correction in the other two groups. The amount and rate of LLC correction could be explained by settling of skeletal discrepancies or LLC at pretreatment with goodness of fit percentages of approximately 82% and 41%, respectively. Orthognathic surgery resulted in significant correction of LLC in Class III craniofacial asymmetry patients, while OCT did not.

Disseminating Metaproteomic Informatics Capabilities and Knowledge Using the Galaxy-P Framework

PubMed Central

Easterly, Caleb; Gruening, Bjoern; Johnson, James; Kolmeder, Carolin A.; Kumar, Praveen; May, Damon; Mehta, Subina; Mesuere, Bart; Brown, Zachary; Elias, Joshua E.; Hervey, W. Judson; McGowan, Thomas; Muth, Thilo; Rudney, Joel; Griffin, Timothy J.

2018-01-01

The impact of microbial communities, also known as the microbiome, on human health and the environment is receiving increased attention. Studying translated gene products (proteins) and comparing metaproteomic profiles may elucidate how microbiomes respond to specific environmental stimuli, and interact with host organisms. Characterizing proteins expressed by a complex microbiome and interpreting their functional signature requires sophisticated informatics tools and workflows tailored to metaproteomics. Additionally, there is a need to disseminate these informatics resources to researchers undertaking metaproteomic studies, who could use them to make new and important discoveries in microbiome research. The Galaxy for proteomics platform (Galaxy-P) offers an open source, web-based bioinformatics platform for disseminating metaproteomics software and workflows. Within this platform, we have developed easily-accessible and documented metaproteomic software tools and workflows aimed at training researchers in their operation and disseminating the tools for more widespread use. The modular workflows encompass the core requirements of metaproteomic informatics: (a) database generation; (b) peptide spectral matching; (c) taxonomic analysis and (d) functional analysis. Much of the software available via the Galaxy-P platform was selected, packaged and deployed through an online metaproteomics “Contribution Fest“ undertaken by a unique consortium of expert software developers and users from the metaproteomics research community, who have co-authored this manuscript. These resources are documented on GitHub and freely available through the Galaxy Toolshed, as well as a publicly accessible metaproteomics gateway Galaxy instance. These documented workflows are well suited for the training of novice metaproteomics researchers, through online resources such as the Galaxy Training Network, as well as hands-on training workshops. Here, we describe the metaproteomics tools available within these Galaxy-based resources, as well as the process by which they were selected and implemented in our community-based work. We hope this description will increase access to and utilization of metaproteomics tools, as well as offer a framework for continued community-based development and dissemination of cutting edge metaproteomics software. PMID:29385081

Disseminating Metaproteomic Informatics Capabilities and Knowledge Using the Galaxy-P Framework.

PubMed

Blank, Clemens; Easterly, Caleb; Gruening, Bjoern; Johnson, James; Kolmeder, Carolin A; Kumar, Praveen; May, Damon; Mehta, Subina; Mesuere, Bart; Brown, Zachary; Elias, Joshua E; Hervey, W Judson; McGowan, Thomas; Muth, Thilo; Nunn, Brook; Rudney, Joel; Tanca, Alessandro; Griffin, Timothy J; Jagtap, Pratik D

2018-01-31

The impact of microbial communities, also known as the microbiome, on human health and the environment is receiving increased attention. Studying translated gene products (proteins) and comparing metaproteomic profiles may elucidate how microbiomes respond to specific environmental stimuli, and interact with host organisms. Characterizing proteins expressed by a complex microbiome and interpreting their functional signature requires sophisticated informatics tools and workflows tailored to metaproteomics. Additionally, there is a need to disseminate these informatics resources to researchers undertaking metaproteomic studies, who could use them to make new and important discoveries in microbiome research. The Galaxy for proteomics platform (Galaxy-P) offers an open source, web-based bioinformatics platform for disseminating metaproteomics software and workflows. Within this platform, we have developed easily-accessible and documented metaproteomic software tools and workflows aimed at training researchers in their operation and disseminating the tools for more widespread use. The modular workflows encompass the core requirements of metaproteomic informatics: (a) database generation; (b) peptide spectral matching; (c) taxonomic analysis and (d) functional analysis. Much of the software available via the Galaxy-P platform was selected, packaged and deployed through an online metaproteomics "Contribution Fest" undertaken by a unique consortium of expert software developers and users from the metaproteomics research community, who have co-authored this manuscript. These resources are documented on GitHub and freely available through the Galaxy Toolshed, as well as a publicly accessible metaproteomics gateway Galaxy instance. These documented workflows are well suited for the training of novice metaproteomics researchers, through online resources such as the Galaxy Training Network, as well as hands-on training workshops. Here, we describe the metaproteomics tools available within these Galaxy-based resources, as well as the process by which they were selected and implemented in our community-based work. We hope this description will increase access to and utilization of metaproteomics tools, as well as offer a framework for continued community-based development and dissemination of cutting edge metaproteomics software.

Research and Implementation of Key Technologies in Multi-Agent System to Support Distributed Workflow

NASA Astrophysics Data System (ADS)

Pan, Tianheng

2018-01-01

In recent years, the combination of workflow management system and Multi-agent technology is a hot research field. The problem of lack of flexibility in workflow management system can be improved by introducing multi-agent collaborative management. The workflow management system adopts distributed structure. It solves the problem that the traditional centralized workflow structure is fragile. In this paper, the agent of Distributed workflow management system is divided according to its function. The execution process of each type of agent is analyzed. The key technologies such as process execution and resource management are analyzed.

Progress in digital color workflow understanding in the International Color Consortium (ICC) Workflow WG

NASA Astrophysics Data System (ADS)

McCarthy, Ann

2006-01-01

The ICC Workflow WG serves as the bridge between ICC color management technologies and use of those technologies in real world color production applications. ICC color management is applicable to and is used in a wide range of color systems, from highly specialized digital cinema color special effects to high volume publications printing to home photography. The ICC Workflow WG works to align ICC technologies so that the color management needs of these diverse use case systems are addressed in an open, platform independent manner. This report provides a high level summary of the ICC Workflow WG objectives and work to date, focusing on the ways in which workflow can impact image quality and color systems performance. The 'ICC Workflow Primitives' and 'ICC Workflow Patterns and Dimensions' workflow models are covered in some detail. Consider the questions, "How much of dissatisfaction with color management today is the result of 'the wrong color transformation at the wrong time' and 'I can't get to the right conversion at the right point in my work process'?" Put another way, consider how image quality through a workflow can be negatively affected when the coordination and control level of the color management system is not sufficient.

77 FR 20451 - Self-Regulatory Organizations; BATS Exchange, Inc.; BATS Y-Exchange, Inc.; NASDAQ OMX BX, Inc...

Federal Register 2010, 2011, 2012, 2013, 2014

2012-04-04

... Industry Regulatory Authority, Inc.; International Securities Exchange LLC; The NASDAQ Stock Market LLC... Whether To Disapprove Proposed Rule Changes Relating to Trading Halts Due to Extraordinary Market.... (``FINRA''), International Securities Exchange LLC (``ISE''), The NASDAQ Stock Market LLC (``Nasdaq...

78 FR 20149 - Sage Quant Management LLC, et al.; Notice of Application

Federal Register 2010, 2011, 2012, 2013, 2014

2013-04-03

... Quant Management LLC, et al.; Notice of Application March 28, 2013. AGENCY: Securities and Exchange...)(1)(A) and 12(d)(1)(B) of the Act. Applicants: Sage Quant Management LLC (``Adviser''), and Sage Quant ETF Trust (``Trust'') and ETF Distributors LLC. SUMMARY: Summary of Application: Applicants...

77 FR 276 - Combined Notice of Filings #1

Federal Register 2010, 2011, 2012, 2013, 2014

2012-01-04

... Energy Storage, LLC, AES ES Westover, LLC, AES Ironwood, L.L.C., AES Red Oak, L.L.C., AES Laurel Mountain... DEPARTMENT OF ENERGY Federal Energy Regulatory Commission Combined Notice of Filings 1 Take notice... Energy Marketing Company, Ameren Energy Generating Company, Electric Energy Inc., AmerenEnergy Medina...

76 FR 41773 - Combined Notice of Filings #3

Federal Register 2010, 2011, 2012, 2013, 2014

2011-07-15

... Eastern Energy, LP, AES Energy Storage, LLC, AES Alamitos, LLC, AES Redondo Beach, L.L.C., Condon Wind... DEPARTMENT OF ENERGY Federal Energy Regulatory Commission Combined Notice of Filings 3 Take notice...-2596-001, ER10-2597-001. Applicants: BP Energy Company, Fowler Ridge II Wind Farm LLC, Fowler Ridge III...

76 FR 21743 - Indigo Logistics, LLC, Liliya Ivanenko, and Leonid Ivanenko-Possible Violations of Section 19 of...

Federal Register 2010, 2011, 2012, 2013, 2014

2011-04-18

... FEDERAL MARITIME COMMISSION [Docket No. 11-06] Indigo Logistics, LLC, Liliya Ivanenko, and Leonid... Order of Investigation and Hearing entitled Indigo Logistics, LLC; Liliya Ivanenko; and Leonid Ivanenko... instituted to determine: (1) Whether Indigo Logistics, LLC, Liliya Ivanenko, and Leonid Ivanenko violated...

75 FR 34170 - Chrysler Group LLC, Formally Known as Chrysler LLC, Kenosha Engine Plant, Including On-Site...

Federal Register 2010, 2011, 2012, 2013, 2014

2010-06-16

... automobile engines. The company reports that workers leased from Caravan Knight Facilities Management, LLC..., Formally Known as Chrysler LLC, Kenosha Engine Plant, Including On-Site Leased Workers From Caravan Knight..., Kenosha Engine Plant, Kenosha, Wisconsin. The notice was published in [[Page 34171

75 FR 62421 - Notice of Lodging of Consent Decree Under the Clean Air Act

Federal Register 2010, 2011, 2012, 2013, 2014

2010-10-08

... States of America v. James Valley Ethanol, LLC, Northern Lights Ethanol, LLC, and Poet Plant Management... Ethanol, LLC (``James Valley''), Northern Lights Ethanol, LLC (``Northern Lights''), and POET Plant.... Defendant James Valley owns an ethanol production facility in Brown County, South Dakota, near Groton (the...

75 FR 57754 - Combined Notice of Filings No. 2

Federal Register 2010, 2011, 2012, 2013, 2014

2010-09-22

... Refund Report filings: Docket Numbers: RP10-1258-001. Applicants: Central New York Oil and Gas Co., LLC. Description: Central New York Oil and Gas Company, LLC submits their compliance filing to incorporate in the...-001. Applicants: Arlington Storage Company, LLC. Description: Arlington Storage Company, LLC submits...

76 FR 53672 - Combined Notice of Filings #2

Federal Register 2010, 2011, 2012, 2013, 2014

2011-08-29

.... Applicants: PJM Interconnection, L.L.C. Description: PJM Interconnection, L.L.C. submits tariff filing per 35... Time on Monday, September 12, 2011. Docket Numbers: ER11-4343-000. Applicants: PJM Interconnection, L.L.C. Description: PJM Interconnection, L.L.C. submits tariff filing per 35.13(a)(2)(iii: Certificate...

76 FR 8725 - Notice of Baseline Filings

Federal Register 2010, 2011, 2012, 2013, 2014

2011-02-15

... DEPARTMENT OF ENERGY Federal Energy Regulatory Commission Notice of Baseline Filings Enstor Grama Ridge Storage and Docket No. PR10-97-002. Transportation, L.L.C.. EasTrans, LLC Docket No. PR10-30-001. DCP Guadalupe Pipeline, LLC...... Docket No. PR10-31-002. DCP Raptor Pipeline, LLC......... Docket No...

78 FR 9682 - Combined Notice of Filings #2

Federal Register 2010, 2011, 2012, 2013, 2014

2013-02-11

... Wholesale Generator Status of Niagara Wind Power, LLC. Filed Date: 1/31/13. Accession Number: 20130131-5139... Bay Wind, LLC, Vasco Winds, LLC, Victory Garden Phase IV, LLC, Waymart Wind Farm, L.P., Wessington... that the Commission received the following exempt wholesale generator filings: Docket Numbers: EG13-15...

76 FR 23320 - Combined Notice of Filings #2

Federal Register 2010, 2011, 2012, 2013, 2014

2011-04-26

...: Dempsey Ridge Wind Farm, LLC. Description: EWG Self-Certification Dempsey Ridge Wind Farm, LLC. Filed Date.... Eastern Time on Tuesday, May 10, 2011. Docket Numbers: ER11-3391-000. Applicants: Dempsey Ridge Wind Farm, LLC. Description: Dempsey Ridge Wind Farm, LLC submits tariff filing per 35.12: Application for Market...

75 FR 70690 - Visteon Corporation Springfield Plant Formerly Known as VC Regional Assembly & Manufacturing, LLC...

Federal Register 2010, 2011, 2012, 2013, 2014

2010-11-18

... Springfield Plant Formerly Known as VC Regional Assembly & Manufacturing, LLC Including On-Site Leased Workers... formerly known as VC Regional Assembly & Manufacturing, LLC. Workers separated from employment at the... name VC Regional Assembly & Manufacturing, LLC. Accordingly, the Department is amending this...

75 FR 13529 - Combined Notice of Filings #1

Federal Register 2010, 2011, 2012, 2013, 2014

2010-03-22

... DEPARTMENT OF ENERGY Federal Energy Regulatory Commission Combined Notice of Filings 1 March 15..., 2010. Docket Numbers: ER09-1099-003; ER07-412-004. Applicants: Empire Generating Co, LLC; ECP Energy I, LLC. Description: Quarterly Report of ECP Energy I, LLC, and Empire Generating Co, LLC. Filed Date: 02...

75 FR 56613 - Self-Regulatory Organizations; BATS Exchange, Inc.; NASDAQ OMX BX, Inc.; Chicago Board Options...

Federal Register 2010, 2011, 2012, 2013, 2014

2010-09-16

... Stock Exchange, Inc.; EDGA Exchange, Inc.; EDGX Exchange, Inc.; International Securities Exchange LLC; The NASDAQ Stock Market LLC; National Stock Exchange, Inc.; New York Stock Exchange LLC; NYSE Amex LLC...''), NASDAQ OMX BX, Inc. (``BX''), Chicago Board Options Exchange, Incorporated (``CBOE''), Chicago Stock...

76 FR 45790 - Combined Notice of Filings #1

Federal Register 2010, 2011, 2012, 2013, 2014

2011-08-01

.... Applicants: El Dorado Energy, LLC, Copper Mountain Solar 1, LLC. Description: El Dorardo Energy, LLC and... Mountain Solar 1 LLC FERC Electric Tariff No. 1 Market- Based Rates Tariff to be effective 7/14/2011. Filed... DEPARTMENT OF ENERGY Federal Energy Regulatory Commission Combined Notice of Filings 1 Take notice...

77 FR 42831 - Professional Transportation, Inc.-Asset Acquisition-CUSA ES, LLC and CUSA CSS, LLC

Federal Register 2010, 2011, 2012, 2013, 2014

2012-07-20

... DEPARTMENT OF TRANSPORTATION Surface Transportation Board [Docket No. MCF 21046] Professional Transportation, Inc.--Asset Acquisition--CUSA ES, LLC and CUSA CSS, LLC AGENCY: Surface Transportation Board, Department of Transportation. ACTION: Correction to Notice of Finance Application. On June 29, 2012, notice...

78 FR 70297 - Combined Notice of Filings #1

Federal Register 2010, 2011, 2012, 2013, 2014

2013-11-25

.... Applicants: Black Bear Hydro Partners, LLC, Black Bear Development Holdings, LLC, Black Bear SO, LLC, Black Bear Holding LLC. Description: Joint Application for Authorization Under Section 203 of Black Bear...: 20131114-5164. Comments Due: 5 p.m. ET 12/5/13. Docket Numbers: ER14-407-000. Applicants: Capacity Markets...

78 FR 70580 - Apria Healthcare LLC, Billing Department, Overland Park, Kansas; Notice of Negative Determination...

Federal Register 2010, 2011, 2012, 2013, 2014

2013-11-26

... DEPARTMENT OF LABOR Employment and Training Administration [TA-W-82,838] Apria Healthcare LLC... Reconsideration By application dated September 19, 2013, a former worker of Apria Healthcare LLC, Billing...), applicable to workers and former workers of Apria Healthcare LLC, Billing Department, Overland Park, Kansas...

77 FR 41806 - RG Steel Sparrows Point LLC, Formerly Known as Severstal Sparrows Point LLC, a Subsidiary of RG...

Federal Register 2010, 2011, 2012, 2013, 2014

2012-07-16

... Leased Workers From Echelon Service Company, Sun Associated Industries, INC., MPI Consultants LLC... International, including on-site leased workers from Echelon Service Company, Sun Associated Industries, Inc... Company, Sun Associated Industries, Inc., MPI Consultants LLC, Alliance Engineering, Inc., Washington...

78 FR 53452 - Notice Announcing Filing Priority for Preliminary Permit Applications

Federal Register 2010, 2011, 2012, 2013, 2014

2013-08-29

... DEPARTMENT OF ENERGY Federal Energy Regulatory Commission Notice Announcing Filing Priority for Preliminary Permit Applications Lock+ Hydro Friends Fund XXX, LLC.. Project No. 13625-003 FFP Project 121, LLC.... FFP Project 121, LLC Project No. 14504-000 2. Lock+ Hydro Friends Fund XXX, LLC Project No. 13625-003...

78 FR 18402 - Self-Regulatory Organizations; New York Stock Exchange LLC; NYSE MKT LLC; Notice of Designation...

Federal Register 2010, 2011, 2012, 2013, 2014

2013-03-26

...-4 thereunder,\\2\\ proposed rule changes to allow Retail Member Organizations (``RMOs'') to attest...-regulatory organization consents, the Commission shall either approve the proposed rule change, disapprove...-07] Self-Regulatory Organizations; New York Stock Exchange LLC; NYSE MKT LLC; Notice of Designation...

77 FR 16543 - Combined Notice of Filings #1

Federal Register 2010, 2011, 2012, 2013, 2014

2012-03-21

...: Docket Numbers: ER10-2179-007; ER10-2181-007; ER10-2182-007. Applicants: R.E. Ginna Nuclear Power Plant, LLC, Nine Mile Point Nuclear Station, LLC, Calvert Cliffs Nuclear Power Plant, LLC. Description.... Applicants: Pioneer Trail Wind Farm, LLC. Description: Application for Authorization Under Section 203 of the...

75 FR 41524 - Horton Manufacturing Company, LLC, Tallmadge, OH; Amended Certification Regarding Eligibility To...

Federal Register 2010, 2011, 2012, 2013, 2014

2010-07-16

... employees of Horton Manufacturing Company, LLC and were not employees of either Wildcomm-Horton Partners... Company, LLC, Tallmadge, OH; Amended Certification Regarding Eligibility To Apply for Worker Adjustment... 11, 2010, applicable to workers of Horton Archery, LLC, formerly known as Wildcomm-Horton Partners...

77 FR 14357 - Combined Notice of Filings #1

Federal Register 2010, 2011, 2012, 2013, 2014

2012-03-09

...-1000-008. Applicants: Las Vegas Power Company, LLC, Griffith Energy LLC, LS Power Marketing, LLC. Description: Third Supplement to Updated Market Power Analysis of LS Power Marketing, LLC, et al. Filed Date...: Southwest Power Pool, Inc. Description: Integrated Marketplace to be effective 3/1/2014. Filed Date: 2/29/12...

75 FR 76721 - Combined Notice of Filings No. 1

Federal Register 2010, 2011, 2012, 2013, 2014

2010-12-09

.... Docket Numbers: ER11-2201-000. Applicants: Evergreen Wind Power III, LLC. Description: Evergreen Wind Power III, LLC submits tariff filing per 35.12: MBR Application of Evergreen Wind Power III, LLC to be... Tuesday, December 21, 2010. Docket Numbers: ER11-2212-000. Applicants: Oak Creek Wind Power, LLC...

75 FR 8685 - Combined Notice of Filings #1

Federal Register 2010, 2011, 2012, 2013, 2014

2010-02-25

...: EC10-47-000. Applicants: Ashtabula Wind II, LLC. Description: Ashtabula Wind II, LLC et al. requests... Service Company submits supplement to the Triennial Market Power Analysis filed on 7/31/09. Filed Date: 02... Corporation; Hot Spring Power Company, LLC; Mt. Tom Generating Company; Choctaw Gas Generation, LLC; Hopewell...

78 FR 24191 - Combined Notice of Filings #1

Federal Register 2010, 2011, 2012, 2013, 2014

2013-04-24

.... Applicants: Alta Wind X, LLC, Alta Wind XI, LLC, Alta Windpower Development, LLC. Description: Application... Alta Wind X, LLC, et al. Filed Date: 4/15/13. Accession Number: 20130415-5207. Comments Due: 5 p.m. ET.... Applicants: Midwest Independent Transmission System Operator, Inc. Description: 04-15-2013 SA 2455 DEGS Wind...

76 FR 58258 - Combined Notice of Filings #1

Federal Register 2010, 2011, 2012, 2013, 2014

2011-09-20

... Colorado Wind Energy, LLC, Peetz Table Energy, LLC, NextEtra Energy Power Marketing, LLC. Description: Next... System Cost filing for sales of electric power to the Bonneville Power Administration pursuant to Part 35...-832-011. Applicants: ESI Vansycle Partners, L.P., FPL Energy New Mexico Wind, LLC, FPL Energy...

76 FR 45878 - Alticor, Inc., Including Access Business Group International LLC and Amway Corporation, Buena...

Federal Register 2010, 2011, 2012, 2013, 2014

2011-08-01

...,420B] Alticor, Inc., Including Access Business Group International LLC and Amway Corporation, Buena Park, CA; Alticor, Inc., Including Access Business Group International LLC and Amway Corporation...., Including Access Business Group International LLC and Amway Corporation, Including On-Site Leased Workers...

75 FR 32221 - Alticor, Inc., Including Access Business Group International, LLC, and Amway Corporation...

Federal Register 2010, 2011, 2012, 2013, 2014

2010-06-07

... Access Business Group International, LLC, and Amway Corporation, Including On-Site Leased Workers from... Business Group International, LLC and Amway Corporation. The notice was published in the Federal Register... International, LLC and Amway Corporation. The Department has determined that these workers were sufficiently...

75 FR 26794 - Alticor, Inc., Including Access Business Group International LLC and Amway Corporation, Buena...

Federal Register 2010, 2011, 2012, 2013, 2014

2010-05-12

..., Inc., Including Access Business Group International LLC and Amway Corporation, Buena Park, CA; Alticor, Inc., Including Access Business Group International LLC, and Amway Corporation, Ada, MI; Amended... of Alticor, Inc., including Access Business Group International LLC and Amway Corporation, Buena Park...

78 FR 46933 - Combined Notice of Filings #1

Federal Register 2010, 2011, 2012, 2013, 2014

2013-08-02

... Power Act of Saja Energy LLC. Filed Date: 7/24/13. Accession Number: 20130724-5109. Comments Due: 5 p.m.... Applicants: Eagle Point Power Generation LLC, Hazle Spindle, LLC, La Paloma Generating Company, LLC, RC Cape...-001. Applicants: Public Service Company of New Mexico. Description: WestConnect Regional Transmission...

77 FR 8248 - Bluewater Gas Storage, LLC; Notice of Application

Federal Register 2010, 2011, 2012, 2013, 2014

2012-02-14

... DEPARTMENT OF ENERGY Federal Energy Regulatory Commission [Docket No. CP12-51-000] Bluewater Gas Storage, LLC; Notice of Application Take notice that on January 27, 2012, Bluewater Gas Storage, LLC... Wilson Kisluk, Senior Attorney, Bluewater Gas Storage, LLC, 333 Clay Street, Suite 1500, Houston, Texas...

78 FR 51718 - Combined Notice of Filings #1

Federal Register 2010, 2011, 2012, 2013, 2014

2013-08-21

... Energy Marketing LLC, Champion Energy Services, LLC, Champion Energy, LLC. Description: Notice of Change in Status for Champion Energy Marketing LLC, et al. Filed Date: 8/12/13. Accession Number: 20130812....m. ET 8/23/13. The filings are accessible in the Commission's eLibrary system by clicking on the...

78 FR 16569 - Iowa Pacific Holdings, LLC, Permian Basin Railways, and San Luis & Rio Grande Railroad-Corporate...

Federal Register 2010, 2011, 2012, 2013, 2014

2013-03-15

... Exemption-- Massachusetts Coastal Railroad, LLC Iowa Pacific Holdings, LLC (IPH), its wholly owned subsidiaries Permian Basin Railways (PBR) and San Luis & Rio Grande Railroad (SLRG), and Massachusetts Coastal Railroad, LLC (Mass Coastal) (collectively, applicants), have jointly filed a verified notice of exemption...

78 FR 46939 - Notice of Effectiveness of Exempt Wholesale Generator Status

Federal Register 2010, 2011, 2012, 2013, 2014

2013-08-02

... No. EG13-23-000 RE Rosamond Two LLC Docket No. EG13-24-000 Petra Nova Power I LLC....... Docket No... No. EG13-27-000 CCI Roseton LLC Docket No. EG13-28-000 Imperial Valley Solar 1, LLC. Docket No. EG13...

77 FR 74179 - Stingray Pipeline Company, L.L.C.; Notice of Application

Federal Register 2010, 2011, 2012, 2013, 2014

2012-12-13

... Pipeline Company, L.L.C.; Notice of Application Take notice that on November 26, 2012, Stingray Pipeline Company, L.L.C. (Stingray), 1100 Louisiana, Suite 3300, Houston, Texas 77002, filed in the above... Roney, Manager--Regulatory Affairs, Stingray Pipeline Company, L.L.C., 1100 Louisiana, Suite 3300...

76 FR 358 - Combined Notice of Filings # 1

Federal Register 2010, 2011, 2012, 2013, 2014

2011-01-04

..., 2011. Docket Numbers: ER11-2466-000. Applicants: Juniper Canyon Wind Power LLC. Description: Juniper Canyon Wind Power LLC submits tariff filing per 35.37: Revisions to Market-Based Rate Tariff to be... Wind Power LLC. Description: Klondike Wind Power LLC submits tariff filing per 35.37: Revisions to...

77 FR 74652 - Combined Notice of Filings #1

Federal Register 2010, 2011, 2012, 2013, 2014

2012-12-17

...-1521-001; ER12-1522-001; ER12- 1626-001; ER10-2605-004. Applicants: Agua Caliente Solar, LLC, Alta Wind VII, LLC, Alta Wind IX, LLC, Topaz Solar Farms LLC, Yuma Cogeneration Associates. Description...: Midwest Independent Transmission System Operator, Inc. Description: SA 2431 Glacial Ridge Wind-GRE...

Machine learning in computational biology to accelerate high-throughput protein expression.

PubMed

Sastry, Anand; Monk, Jonathan; Tegel, Hanna; Uhlen, Mathias; Palsson, Bernhard O; Rockberg, Johan; Brunk, Elizabeth

2017-08-15

The Human Protein Atlas (HPA) enables the simultaneous characterization of thousands of proteins across various tissues to pinpoint their spatial location in the human body. This has been achieved through transcriptomics and high-throughput immunohistochemistry-based approaches, where over 40 000 unique human protein fragments have been expressed in E. coli. These datasets enable quantitative tracking of entire cellular proteomes and present new avenues for understanding molecular-level properties influencing expression and solubility. Combining computational biology and machine learning identifies protein properties that hinder the HPA high-throughput antibody production pipeline. We predict protein expression and solubility with accuracies of 70% and 80%, respectively, based on a subset of key properties (aromaticity, hydropathy and isoelectric point). We guide the selection of protein fragments based on these characteristics to optimize high-throughput experimentation. We present the machine learning workflow as a series of IPython notebooks hosted on GitHub (https://github.com/SBRG/Protein_ML). The workflow can be used as a template for analysis of further expression and solubility datasets. ebrunk@ucsd.edu or johanr@biotech.kth.se. Supplementary data are available at Bioinformatics online. © The Author (2017). Published by Oxford University Press. All rights reserved. For Permissions, please email: journals.permissions@oup.com

PeptidePicker: a scientific workflow with web interface for selecting appropriate peptides for targeted proteomics experiments.

PubMed

Mohammed, Yassene; Domański, Dominik; Jackson, Angela M; Smith, Derek S; Deelder, André M; Palmblad, Magnus; Borchers, Christoph H

2014-06-25

One challenge in Multiple Reaction Monitoring (MRM)-based proteomics is to select the most appropriate surrogate peptides to represent a target protein. We present here a software package to automatically generate these most appropriate surrogate peptides for an LC/MRM-MS analysis. Our method integrates information about the proteins, their tryptic peptides, and the suitability of these peptides for MRM which is available online in UniProtKB, NCBI's dbSNP, ExPASy, PeptideAtlas, PRIDE, and GPMDB. The scoring algorithm reflects our knowledge in choosing the best candidate peptides for MRM, based on the uniqueness of the peptide in the targeted proteome, its physiochemical properties, and whether it previously has been observed. The modularity of the workflow allows further extension and additional selection criteria to be incorporated. We have developed a simple Web interface where the researcher provides the protein accession number, the subject organism, and peptide-specific options. Currently, the software is designed for human and mouse proteomes, but additional species can be easily be added. Our software improved the peptide selection by eliminating human error, considering multiple data sources and all of the isoforms of the protein, and resulted in faster peptide selection - approximately 50 proteins per hour compared to 8 per day. Compiling a list of optimal surrogate peptides for target proteins to be analyzed by LC/MRM-MS has been a cumbersome process, in which expert researchers retrieved information from different online repositories and used their own reasoning to find the most appropriate peptides. Our scientific workflow automates this process by integrating information from different data sources including UniProt, Global Proteome Machine, NCBI's dbSNP, and PeptideAtlas, simulating the researchers' reasoning, and incorporating their knowledge of how to select the best proteotypic peptides for an MRM analysis. The developed software can help to standardize the selection of peptides, eliminate human error, and increase productivity. Copyright © 2014 Elsevier B.V. All rights reserved.

Radiology information system: a workflow-based approach.

PubMed

Zhang, Jinyan; Lu, Xudong; Nie, Hongchao; Huang, Zhengxing; van der Aalst, W M P

2009-09-01

Introducing workflow management technology in healthcare seems to be prospective in dealing with the problem that the current healthcare Information Systems cannot provide sufficient support for the process management, although several challenges still exist. The purpose of this paper is to study the method of developing workflow-based information system in radiology department as a use case. First, a workflow model of typical radiology process was established. Second, based on the model, the system could be designed and implemented as a group of loosely coupled components. Each component corresponded to one task in the process and could be assembled by the workflow management system. The legacy systems could be taken as special components, which also corresponded to the tasks and were integrated through transferring non-work- flow-aware interfaces to the standard ones. Finally, a workflow dashboard was designed and implemented to provide an integral view of radiology processes. The workflow-based Radiology Information System was deployed in the radiology department of Zhejiang Chinese Medicine Hospital in China. The results showed that it could be adjusted flexibly in response to the needs of changing process, and enhance the process management in the department. It can also provide a more workflow-aware integration method, comparing with other methods such as IHE-based ones. The workflow-based approach is a new method of developing radiology information system with more flexibility, more functionalities of process management and more workflow-aware integration. The work of this paper is an initial endeavor for introducing workflow management technology in healthcare.

A multioutput LLC-type parallel resonant converter

NASA Astrophysics Data System (ADS)

Liu, Rui; Lee, C. Q.; Upadhyay, Anand K.

1992-07-01

When an LLC-type parallel resonant converter (LLC-PRC) operates above resonant frequency, the switching transistors can be turned off at zero voltage. Further study reveals that the LLC-PRC possesses the advantage of lower converter voltage gain as compared with the conventional PRC. Based on analytic results, a complete set of design curves is obtained, from which a systematic design procedure is developed. Experimental results from a 150 W 150 kHz multioutput LLC-type PRC power supply are presented.

Information Issues and Contexts that Impair Team Based Communication Workflow: A Palliative Sedation Case Study.

PubMed

Cornett, Alex; Kuziemsky, Craig

2015-01-01

Implementing team based workflows can be complex because of the scope of providers involved and the extent of information exchange and communication that needs to occur. While a workflow may represent the ideal structure of communication that needs to occur, information issues and contextual factors may impact how the workflow is implemented in practice. Understanding these issues will help us better design systems to support team based workflows. In this paper we use a case study of palliative sedation therapy (PST) to model a PST workflow and then use it to identify purposes of communication, information issues and contextual factors that impact them. We then suggest how our findings could inform health information technology (HIT) design to support team based communication workflows.

SU-F-E-10: Student-Driven Exploration of Radiographic Material Properties, Phantom Construction, and Clinical Workflows Or: The Extraordinary Life of CANDY MAN

DOE Office of Scientific and Technical Information (OSTI.GOV)

Mahon, RN; Riblett, MJ; Hugo, GD

Purpose: To develop a hands-on learning experience that explores the radiological and structural properties of everyday items and applies this knowledge to design a simple phantom for radiotherapy exercises. Methods: Students were asked to compile a list of readily available materials thought to have radiation attenuation properties similar to tissues within the human torso. Participants scanned samples of suggested materials and regions of interest (ROIs) were used to characterize bulk attenuation properties. Properties of each material were assessed via comparison to a Gammex Tissue characterization phantom and used to construct a list of inexpensive near-tissue-equivalent materials. Critical discussions focusing onmore » samples found to differ from student expectations were used to revise and narrow the comprehensive list. From their newly acquired knowledge, students designed and constructed a simple thoracic phantom for use in a simulated clinical workflow. Students were tasked with setting up the phantom and acquiring planning CT images for use in treatment planning and dose delivery. Results: Under engineer and physicist supervision, students were trained to use a CT simulator and acquired images for approximately 60 different foodstuffs, candies, and household items. Through peer discussion, students gained valuable insights and were made to review preconceptions about radiographic material properties. From a subset of imaged materials, a simple phantom was successfully designed and constructed to represent a human thorax. Students received hands-on experience with clinical treatment workflows by learning how to perform CT simulation, create a treatment plan for an embedded tumor, align the phantom for treatment, and deliver a treatment fraction. Conclusion: In this activity, students demonstrated their ability to reason through the radiographic material selection process, construct a simple phantom to specifications, and exercise their knowledge of clinical workflows. Furthermore, the enjoyable and inexpensive nature of this project proved to attract participant interest and drive creative exploration. Mahon and Riblett have nothing to disclose; Hugo has a research agreement with Phillips Medical systems, a license agreement with Varian Medical Systems, research grants from the National Institute of Health. Authors do not have any potential conflicts of interest to disclose.« less

A Workflow to Improve the Alignment of Prostate Imaging with Whole-mount Histopathology.

PubMed

Yamamoto, Hidekazu; Nir, Dror; Vyas, Lona; Chang, Richard T; Popert, Rick; Cahill, Declan; Challacombe, Ben; Dasgupta, Prokar; Chandra, Ashish

2014-08-01

Evaluation of prostate imaging tests against whole-mount histology specimens requires accurate alignment between radiologic and histologic data sets. Misalignment results in false-positive and -negative zones as assessed by imaging. We describe a workflow for three-dimensional alignment of prostate imaging data against whole-mount prostatectomy reference specimens and assess its performance against a standard workflow. Ethical approval was granted. Patients underwent motorized transrectal ultrasound (Prostate Histoscanning) to generate a three-dimensional image of the prostate before radical prostatectomy. The test workflow incorporated steps for axial alignment between imaging and histology, size adjustments following formalin fixation, and use of custom-made parallel cutters and digital caliper instruments. The control workflow comprised freehand cutting and assumed homogeneous block thicknesses at the same relative angles between pathology and imaging sections. Thirty radical prostatectomy specimens were histologically and radiologically processed, either by an alignment-optimized workflow (n = 20) or a control workflow (n = 10). The optimized workflow generated tissue blocks of heterogeneous thicknesses but with no significant drifting in the cutting plane. The control workflow resulted in significantly nonparallel blocks, accurately matching only one out of four histology blocks to their respective imaging data. The image-to-histology alignment accuracy was 20% greater in the optimized workflow (P < .0001), with higher sensitivity (85% vs. 69%) and specificity (94% vs. 73%) for margin prediction in a 5 × 5-mm grid analysis. A significantly better alignment was observed in the optimized workflow. Evaluation of prostate imaging biomarkers using whole-mount histology references should include a test-to-reference spatial alignment workflow. Copyright © 2014 AUR. Published by Elsevier Inc. All rights reserved.

FAST: A fully asynchronous and status-tracking pattern for geoprocessing services orchestration

NASA Astrophysics Data System (ADS)

Wu, Huayi; You, Lan; Gui, Zhipeng; Gao, Shuang; Li, Zhenqiang; Yu, Jingmin

2014-09-01

Geoprocessing service orchestration (GSO) provides a unified and flexible way to implement cross-application, long-lived, and multi-step geoprocessing service workflows by coordinating geoprocessing services collaboratively. Usually, geoprocessing services and geoprocessing service workflows are data and/or computing intensive. The intensity feature may make the execution process of a workflow time-consuming. Since it initials an execution request without blocking other interactions on the client side, an asynchronous mechanism is especially appropriate for GSO workflows. Many critical problems remain to be solved in existing asynchronous patterns for GSO including difficulties in improving performance, status tracking, and clarifying the workflow structure. These problems are a challenge when orchestrating performance efficiency, making statuses instantly available, and constructing clearly structured GSO workflows. A Fully Asynchronous and Status-Tracking (FAST) pattern that adopts asynchronous interactions throughout the whole communication tier of a workflow is proposed for GSO. The proposed FAST pattern includes a mechanism that actively pushes the latest status to clients instantly and economically. An independent proxy was designed to isolate the status tracking logic from the geoprocessing business logic, which assists the formation of a clear GSO workflow structure. A workflow was implemented in the FAST pattern to simulate the flooding process in the Poyang Lake region. Experimental results show that the proposed FAST pattern can efficiently tackle data/computing intensive geoprocessing tasks. The performance of all collaborative partners was improved due to the asynchronous mechanism throughout communication tier. A status-tracking mechanism helps users retrieve the latest running status of a GSO workflow in an efficient and instant way. The clear structure of the GSO workflow lowers the barriers for geospatial domain experts and model designers to compose asynchronous GSO workflows. Most importantly, it provides better support for locating and diagnosing potential exceptions.

77 FR 12007 - Notice of Petitions by Firms tor Determination of Eligibility to Apply For Trade Adjustment...

Federal Register 2010, 2011, 2012, 2013, 2014

2012-02-28

.../2011 through 02/21/2012] Date accepted for Firm name Address investigation Products Cuisine Holdings Unlimited, 1997 Surgi Dr., 12/29/2011 The firm manufactures food for human LLC.. Mandeville, LA consumption. 70448. Custom Service Printer, lnc.. 916 E. Keating 2/8/2012 The firm manufactures printed products...

77 FR 10490 - FPL Energy Maine Hydro, LLC; Notice of Availability of Environmental Assessment

Federal Register 2010, 2011, 2012, 2013, 2014

2012-02-22

... the spillway at the Upper Dam development, which is part of the Upper and Middle Dam Storage Project... not constitute a major federal action significantly affecting the quality of the human environment... Commission's Web site at http://www.ferc.gov using the ``eLibrary'' link. Enter the docket number excluding...