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Sample records for id link issue

  1. Study IDs 2 Zika Virus Proteins Linked to Microcephaly

    MedlinePlus

    ... gov/news/fullstory_160386.html Study IDs 2 Zika Virus Proteins Linked to Microcephaly Discovery might one ... say they've identified two proteins in the Zika virus linked to the severe birth defect microcephaly. ...

  2. MicroRNAs and intellectual disability (ID) in Down syndrome, X-linked ID, and Fragile X syndrome

    PubMed Central

    Siew, Wei-Hong; Tan, Kai-Leng; Babaei, Maryam Abbaspour; Cheah, Pike-See; Ling, King-Hwa

    2013-01-01

    Intellectual disability (ID) is one of the many features manifested in various genetic syndromes leading to deficits in cognitive function among affected individuals. ID is a feature affected by polygenes and multiple environmental factors. It leads to a broad spectrum of affected clinical and behavioral characteristics among patients. Until now, the causative mechanism of ID is unknown and the progression of the condition is poorly understood. Advancement in technology and research had identified various genetic abnormalities and defects as the potential cause of ID. However, the link between these abnormalities with ID is remained inconclusive and the roles of many newly discovered genetic components such as non-coding RNAs have not been thoroughly investigated. In this review, we aim to consolidate and assimilate the latest development and findings on a class of small non-coding RNAs known as microRNAs (miRNAs) involvement in ID development and progression with special focus on Down syndrome (DS) and X-linked ID (XLID) [including Fragile X syndrome (FXS)]. PMID:23596395

  3. Development Issues on Linked Data Weblog Enrichment

    NASA Astrophysics Data System (ADS)

    Ruiz-Rube, Iván; Cornejo, Carlos M.; Dodero, Juan Manuel; García, Vicente M.

    In this paper, we describe the issues found during the development of LinkedBlog, a Linked Data extension for WordPress blogs. This extension enables to enrich text-based and video information contained in blog entries with RDF triples that are suitable to be stored, managed and exploited by other web-based applications. The issues have to do with the generality, usability, tracking, depth, security, trustiness and performance of the linked data enrichment process. The presented annotation approach aims at maintaining web-based contents independent from the underlying ontological model, by providing a loosely coupled RDFa-based approach in the linked data application. Finally, we detail how the performance of annotations can be improved through a semantic reasoner.

  4. Weighted link graphs: a distributed IDS for secondary intrusion detection and defense

    NASA Astrophysics Data System (ADS)

    Zhou, Mian; Lang, Sheau-Dong

    2005-03-01

    While a firewall installed at the perimeter of a local network provides the first line of defense against the hackers, many intrusion incidents are the results of successful penetration of the firewalls. One computer"s compromise often put the entire network at risk. In this paper, we propose an IDS that provides a finer control over the internal network. The system focuses on the variations of connection-based behavior of each single computer, and uses a weighted link graph to visualize the overall traffic abnormalities. The functionality of our system is of a distributed personal IDS system that also provides a centralized traffic analysis by graphical visualization. We use a novel weight assignment schema for the local detection within each end agent. The local abnormalities are quantitatively carried out by the node weight and link weight and further sent to the central analyzer to build the weighted link graph. Thus, we distribute the burden of traffic processing and visualization to each agent and make it more efficient for the overall intrusion detection. As the LANs are more vulnerable to inside attacks, our system is designed as a reinforcement to prevent corruption from the inside.

  5. 6 CFR 37.71 - Driver's licenses and identification cards issued under section 202(d)(11) of the REAL ID Act.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... HOMELAND SECURITY, OFFICE OF THE SECRETARY REAL ID DRIVER'S LICENSES AND IDENTIFICATION CARDS Driver's Licenses and Identification Cards Issued Under Section 202(d)(11) of the REAL ID Act § 37.71 Driver's licenses and identification cards issued under section 202(d)(11) of the REAL ID Act. (a) Except...

  6. 6 CFR 37.71 - Driver's licenses and identification cards issued under section 202(d)(11) of the REAL ID Act.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... issued under section 202(d)(11) of the REAL ID Act. 37.71 Section 37.71 Domestic Security DEPARTMENT OF HOMELAND SECURITY, OFFICE OF THE SECRETARY REAL ID DRIVER'S LICENSES AND IDENTIFICATION CARDS Driver's Licenses and Identification Cards Issued Under section 202(d)(11) of the REAL ID Act § 37.71...

  7. Corneal Cross-Linking and Safety Issues

    PubMed Central

    Spoerl, Eberhard; Hoyer, Anne; Pillunat, Lutz E; Raiskup, Frederik

    2011-01-01

    Purpose: To compile the safety aspects of the corneal collagen cross-linking (CXL) by means of the riboflavin/UVA (370 nm) approach. Materials and Methodology: Analysis of the current treatment protocol with respect to safety during CXL. Results: The currently used UVA dose density of 5.4 J/cm2 and the corresponding irradiance of 3 mW/cm2 are below the known damage thresholds of UVA for the corneal endothelium, lens, and retina. Regarding the photochemical damages due to the free radicals the damage threshold for endothelial cells is 0.35 mW/cm2. In a 400μm thick corneal stroma saturated with riboflavin, the irradiance at the endothelial level is about 0.18 mW/cm2, which is a factor of 2 smaller than the damage threshold. Conclusion: As long as the corneal stroma treated has a minimal thickness of 400 microns (as recommended), neither corneal endothelium nor deeper structures such as lens and retina will suffer any damages. The light source should provide a homogenous irradiance avoiding hot spots. PMID:21399770

  8. Not Just Another Single Issue: Teen Pregnancy Prevention's Link to Other Critical Social Issues.

    ERIC Educational Resources Information Center

    National Campaign To Prevent Teen Pregnancy, Washington, DC.

    This report discusses critical social issues linked to teen pregnancy, explaining that teen pregnancy prevention should be viewed as working to improve these social issues. After providing general background on teen pregnancy, the report offers five fact sheets: (1) "Teen Pregnancy, Welfare Dependency, and Poverty" (continuing to reduce teen…

  9. Issues Involved in the Development of an Open Standard for Data Link of Aviation Weather Information

    NASA Technical Reports Server (NTRS)

    Grappel, R. D.

    2000-01-01

    This paper describes how an effective and efficient data link system for the dissemination of aviation weather information could be constructed. The system is built upon existing 'open standard' foundations drawn from current aviation and computer technologies. Issues of communications protocols and application data formats are discussed. The proposed aviation weather data link system is dependent of the actual link mechanism selected.

  10. The Ids have it.

    PubMed

    Edwards, Lincoln A; Fine, Howard A

    2010-12-14

    In this issue of Cancer Cell, Anido et al. demonstrate that Id1 is the likely arbiter of divergent transforming growth factor-β (TGF-β) signaling in glioma-initiating cells (GICs) from different tumors. These findings hold both the promise and potential peril of therapeutic targeting of the TGF-β pathway.

  11. ISS/IDS Detector Study

    SciTech Connect

    Cervera-Villanueva, A.

    2008-02-21

    This article summarises the results obtained by the detector working group of the 'International Scooping Study' (ISS) of a future neutrino oscillations facility. Special emphasis is put on far detectors, for which some of the main issues are identified. A detector R and D strategy in the context of the 'International Design Study' (IDS) for a neutrino factory is also presented.

  12. Demographic links to savings in life cycle models: identification of issues for LDCs.

    PubMed

    Raut, L K

    1992-01-01

    The author considers the potential for a link between the recent pattern of demographic transition and intertemporal and inter-country variations in savings rates. Fertility, infant mortality, life expectancy, and levels of female and child labor force participation are among the various demographic factors which affect national savings rates through their effects upon age structure, age-specific individual savings behavior, and their general equilibrium effects upon interest rates, wage rates, and income distribution. The author establishes a simple discrete time life cycle model of savings, explains the issues related to age structure, and discusses the effect of age-specific savings functions, the general equilibrium effects of demographic factors, the effects of life expectancies and child mortalities, and the nature of social security coverages in less developed countries, as well as issues which are especially important for less developed countries. A new strategy for empirically evaluating demographic policies is proposed. That is, one can estimate the age profile of earnings, saving and fertility rates from household survey data. The life tables can then be used to compute the aggregate savings rate and population size. Any demographic policy which affects the fertility rate, life expectancy, and investment in the quality of children will change the aggregate saving and population growth rates. These two aggregate effects could be compared to evaluate demographic policies. The author stresses, however, that changes in different demographic factors will have different short-run and long-run effects upon the savings rate which will also depend upon whether such changes are transitory or permanent.

  13. "Where I Came From, Where I Am Now, and Where I'd Like to Be": Aspiring Administrators Reflect on Issues Related to Equity, Diversity, and Social Justice

    ERIC Educational Resources Information Center

    Hernandez, Frank; Marshall, Joanne M.

    2009-01-01

    This study explores student reflections about issues related to equity, diversity, and social justice from an educational foundations course. Online reflections and course assignments were analyzed from 15 aspiring administrators for patterns. Findings indicate that (1) students were willing to engage and reflect on their experiences and cultural…

  14. A big picture look at big coal: Teaching students to link societal and environmental issues

    NASA Astrophysics Data System (ADS)

    Sojka, S. L.

    2014-12-01

    The environmental impact of coal mining and burning of coal is evident and generally easy to understand. However, students often struggle to understand the social impacts of coal mining. A jigsaw activity culminating in a mock town hall meeting helps students link social, economic and environmental impacts of coal mining. Students are divided into four groups and assigned the task of researching the environmental, social, economic or health impacts of coal mining in West Virginia. When students have completed the research, they are assigned a role for the town hall. Roles include local community members, direct employees of the coal industry, business owners from industries related to coal mining, and environmentalists. One student from each research area is assigned to each role, forcing students to consider environmental, social, health and economic aspects of coal mining in choosing an appropriate position for their role. Students have 30 minutes to prepare their positions and then present for 2-5 minutes in the simulated town hall. We then have open class discussion and review the positions. Finally, students are required to write a letter to the editor of the local paper. The specific topic for the town hall and letters can be varied based on current events and could include new regulations on power plants, mine safety, government funding of alternative energy supplies or a range of other topics. This approach forces students to consider all aspects of the issue. In addition, because students have to assume a role, they are more aware of the direct impact that coal mining has on individuals' lives.

  15. The Now Frontier. Linking Earth and Planets. Issue No. 1-4.

    ERIC Educational Resources Information Center

    Jet Propulsion Lab., Pasadena, CA.

    This publication includes four pamphlets providing background material for understanding the NASA program of planetary flights. Each issue presents student involvement activities as well as suggested reading lists. Issue 1 describes the innermost planets of the solar system. Issue 2 gives information about the evolution of the planetary system as…

  16. The Now Frontier. Pioneer to Jupiter. Man Links Earth and Planets. Issue No. 1-5.

    ERIC Educational Resources Information Center

    1973

    This packet of space science instructional materials includes five issues related to the planet Jupiter. Each issue presents factual material about the planet, diagramatic representations of its movements and positions relative to bright stars or the earth, actual photographs and/or tables of data collected relevant to Pioneer 10, the spacecraft…

  17. Preparing Students for College and Career: Linked Learning in California. Issue Brief

    ERIC Educational Resources Information Center

    Richmond, Eric

    2010-01-01

    To prepare students for success in life, the twenty-first-century American high school needs to shift its focus from preparing for college "or" career to achieving college "and" career readiness for every student. In some areas of the country, progress is being made on this front. One of the most comprehensive efforts is the Linked Learning…

  18. Genetics Experts Unite to I.D. Unknown Katrina Victims

    MedlinePlus

    ... Current Issue Past Issues Research News From NIH Genetics Experts Unite to I.D. Unknown Katrina Victims ... in the recent Advances in molecular biology and genetics," says team member Stephen Sherry, Ph.D., of ...

  19. Equity: The Critical Link in Southern Economic Development. Cross-Cutting Issue Report No. 2.

    ERIC Educational Resources Information Center

    Southern Growth Policies Board, Research Triangle Park, NC.

    This report presents a general overview of equity issues in the modern South. It discusses access to such needs as jobs and job opportunities, health, housing, education, government benefits, political power, resources, and protection. Six at-risk groups receive special focus: the poor; Blacks; Hispanics; women; older Southerners; and the…

  20. Linking Work-Family Issues to the Bottom Line. Report Number 962.

    ERIC Educational Resources Information Center

    Friedman, Dana E.

    A 1988 symposium on the effect of family problems on the corporate bottom line and a review of more than 80 other studies have confirmed that business investments in programs and policies to resolve family-work conflicts yield returns. Family issues/problems have been documented to affect employee recruitment, productivity, turnover, and…

  1. Large system change challenges: addressing complex critical issues in linked physical and social domains

    NASA Astrophysics Data System (ADS)

    Waddell, Steve; Cornell, Sarah; Hsueh, Joe; Ozer, Ceren; McLachlan, Milla; Birney, Anna

    2015-04-01

    Most action to address contemporary complex challenges, including the urgent issues of global sustainability, occurs piecemeal and without meaningful guidance from leading complex change knowledge and methods. The potential benefit of using such knowledge is greater efficacy of effort and investment. However, this knowledge and its associated tools and methods are under-utilized because understanding about them is low, fragmented between diverse knowledge traditions, and often requires shifts in mindsets and skills from expert-led to participant-based action. We have been engaged in diverse action-oriented research efforts in Large System Change for sustainability. For us, "large" systems can be characterized as large-scale systems - up to global - with many components, of many kinds (physical, biological, institutional, cultural/conceptual), operating at multiple levels, driven by multiple forces, and presenting major challenges for people involved. We see change of such systems as complex challenges, in contrast with simple or complicated problems, or chaotic situations. In other words, issues and sub-systems have unclear boundaries, interact with each other, and are often contradictory; dynamics are non-linear; issues are not "controllable", and "solutions" are "emergent" and often paradoxical. Since choices are opportunity-, power- and value-driven, these social, institutional and cultural factors need to be made explicit in any actionable theory of change. Our emerging network is sharing and building a knowledge base of experience, heuristics, and theories of change from multiple disciplines and practice domains. We will present our views on focal issues for the development of the field of large system change, which include processes of goal-setting and alignment; leverage of systemic transitions and transformation; and the role of choice in influencing critical change processes, when only some sub-systems or levels of the system behave in purposeful ways

  2. Not Another ID Model.

    ERIC Educational Resources Information Center

    Roberts, Stephanie; Conn, Cynthia; Lohr, Linda; Hunt, Erin; Duffy, Annmari

    2003-01-01

    Presents a meta-model of instructional design (ID) that integrates content covered in instructional technology graduate programs and explores the impact of an instructional designer's perspective on designs. Discusses the theoretical background; instructional strategies; educational philosophies; paradigm shifts; behaviorist, constructivist, and…

  3. OrbId

    PubMed Central

    Filshtein, Teresa J.; Mackenzie, Craig O.; Dale, Maurice D.; Dela-Cruz, Paul S.; Ernst, Dale M.; Frankenberger, Edward A.; He, Chunyan; Heath, Kaylee L.; Jones, Andria S.; Jones, Daniel K.; King, Edward R.; Maher, Maggie B.; Mitchell, Travis J.; Morgan, Rachel R.; Sirobhushanam, Sirisha; Halkyard, Scott D.; Tiwari, Kiran B.; Rubin, David A.; Borchert, Glen M.; Larson, Erik D.

    2012-01-01

    MicroRNAs coordinate networks of mRNAs, but predicting specific sites of interactions is complicated by the very few bases of complementarity needed for regulation. Although efforts to characterize the specific requirements for microRNA (miR) regulation have made some advances, no general model of target recognition has been widely accepted. In this work, we describe an entirely novel approach to miR target identification. The genomic events responsible for the creation of individual miR loci have now been described with many miRs now known to have been initially formed from transposable element (TE) sequences. In light of this, we propose that limiting miR target searches to transcripts containing a miR’s progenitor TE can facilitate accurate target identification. In this report we outline the methodology behind OrbId (Origin-based identification of microRNA targets). In stark contrast to the principal miR target algorithms (which rely heavily on target site conservation across species and are therefore most effective at predicting targets for older miRs), we find OrbId is particularly efficacious at predicting the mRNA targets of miRs formed more recently in evolutionary time. After defining the TE origins of > 200 human miRs, OrbId successfully generated likely target sets for 191 predominately primate-specific human miR loci. While only a handful of the loci examined were well enough conserved to have been previously evaluated by existing algorithms, we find ~80% of the targets for the oldest miR (miR-28) in our analysis contained within the principal Diana and TargetScan prediction sets. More importantly, four of the 15 OrbId miR-28 putative targets have been previously verified experimentally. In light of OrbId proving best-suited for predicting targets for more recently formed miRs, we suggest OrbId makes a logical complement to existing, conservation based, miR target algorithms. PMID:23087843

  4. Inhibitor of differentiation-4 (Id4) stimulates pigmentation in melanoma leading to histiocyte infiltration.

    PubMed

    DiVito, Kyle A; Trabosh, Valerie A; Chen, You-Shin; Simbulan-Rosenthal, Cynthia M; Rosenthal, Dean S

    2015-02-01

    TGF-β and the inhibitors of differentiation (Id) are linked. Smad7 and other TGF-β inhibitors can potently suppress melanomagenesis; however, little work examining Ids has been reported in melanoma, particularly for Id4. Here, we report that Id4, but not Id2 or Id3 expression, surprisingly, activated robust melanin production in xenografts of previously amelanotic (lacking pigment) 1205Lu/Smad7 (S7) cells. Fontana-Masson stain and de-novo expression of MART-1 and tyrosinase proteins confirmed melanin production. Additionally, pigment-laden CD163+ mouse histiocytes with areas of extensive necrosis were found throughout S7/Id4 tumors, but not in parental 1205Lu, S7/Id2 or S7Id3-derived tumors. Mechanistic investigation revealed increased nuclear M-microphthalmia-associated transcription factor (MITF) and MART-1 promoter activation following Id4 expression in 1205Lu and WM852 melanoma cells, suggesting broader implications for Id4 in melanin synthesis. In human tumors, melanin colocalized with Id4 expression establishing a correlation. Current chemotherapeutics for melanoma are only marginally effective. Immunotherapy provides the most promise, yet the role of innate immunity is poorly understood. Here, TGF-β suppression followed by Id4 expression results in extensive melanin synthesis and robust histiocyte recruitment following tumorigenesis, a novel role for Id4. Our results suggest that TGF-β suppression coupled with pigment overproduction triggers an innate immune response resulting in tumor necrosis.

  5. DHSIsotopeID

    2007-12-18

    DHSIsotopeID is an application designed to read and analyze radiation measurement files taken by radiation measurement files taken by radiation portal monitors, and in particular, by the advanced spectroscopic portals. It requires that the data files be in the N42 file format, compliant with the interface control documents for DNDO radiation measurement files. It carries out an automated analysis to determine which isotopes are present in the spectra, and then presents the results in graphicalmore » form to the user. It also enables further post-processing and analysis, for example by performing further analysis on selected regions of interest of the spectrum, as designated by the user via the graphical interface.« less

  6. New security system for ID certificates in IT society

    NASA Astrophysics Data System (ADS)

    Nagashima, Hisato; Saito, Kazuharu

    2004-06-01

    This paper introduces a new security solution regarding security documents with secure unique information. Our newly proposed security measure enables outputting ID documents by commercially available printer. On this basis, a citizen can apply and accept his ID certificates to and from Issuing Authority via website. A unique gradational latent image emerges if a third party authenticates it under Infrared ray. The principle of this new measure lies in the complicated microstructure generated by our specially designed software. It is understood that its security feature based on secure software and wide applicability for commercially available printers show profound potentiality to construct new security system for ID documents in IT society.

  7. IDS contribution to ITRF2008

    NASA Astrophysics Data System (ADS)

    Valette, J.; Lemoine, F.; Ferrage, P.; Altamimi, Z.; Willis, P.; Stepanek, P.; Otten, M.; Govind, R.; Kuzin, S.; Le Bail, K.; Moore, P.; Yaya, P.; Soudarin, L.

    2009-12-01

    The International DORIS Service (IDS), in operation since 2003, submitted three sets of solutions to ITRF2005 from the IGN/JPL, LEGOS/CLS, and INASAN analysis centers, but no DORIS technique combination. Since that time new analysis centers have become operational including the Geodetic Observatory Pecny (GOP), the European Space Operations Center (ESOC), Geoscience Australia (GAU), the NASA Goddard Space Flight Center (GSC), and the University of Newcastle (NCL). These analysis centers run different software, including Gypsy (IGN & INASAN), GINS (LCA), Bernese (GOP), NAPEOS (ESOC), GEODYN (Geoscience Australia and NASA GSFC) and FAUST (NCL). In order to contribute to ITRF2008, seven analysis centers processed DORIS data from TOPEX/Poseidon, SPOT2, SPOT3, SPOT4, SPOT5, and ENVISAT from 1992 to 2008, producing weekly SINEX solutions or normal equations. The weekly SINEX files from seven AC’s were processed with the CATREF software. Three iterations of an IDS weekly combined time series were completed. The IDS-1, and IDS-2 combinations were preliminary station-only solutions. In the final combination, IDS-3, both stations and the Earth Orientation Paramters (EOP’s) were adjusted. Between each of the IDS combinations, the combination strategy (station filtering, outliers, weighting, scale or geocenter contributions) was improved and the AC’s SINEX series were refined. Some series were extended in data span while others were recomputed to correct anomalies or to improve the quality of the submissions, based on feedback from the combination analyses and intercenter comparisons. For example in IDS-1, both the GAU and GSC solutions were affected by a 20 mm scale offset that was removed in IDS-2 and IDS-3 after the application of improved troposphere modelling in the GEODYN software. The analysis for IDS-1 showed a higher station position WRMS in the vicinity of the high solar flux periods (late 2001-2002). Consequently for IDS-2, several AC’s (LCA, GAU, GSC

  8. SNP ID-info: SNP ID searching and visualization platform.

    PubMed

    Yang, Cheng-Hong; Chuang, Li-Yeh; Cheng, Yu-Huei; Wen, Cheng-Hao; Chang, Phei-Lang; Chang, Hsueh-Wei

    2008-09-01

    Many association studies provide the relationship between single nucleotide polymorphisms (SNPs), diseases and cancers, without giving a SNP ID, however. Here, we developed the SNP ID-info freeware to provide the SNP IDs within inputting genetic and physical information of genomes. The program provides an "SNP-ePCR" function to generate the full-sequence using primers and template inputs. In "SNPosition," sequence from SNP-ePCR or direct input is fed to match the SNP IDs from SNP fasta-sequence. In "SNP search" and "SNP fasta" function, information of SNPs within the cytogenetic band, contig position, and keyword input are acceptable. Finally, the SNP ID neighboring environment for inputs is completely visualized in the order of contig position and marked with SNP and flanking hits. The SNP identification problems inherent in NCBI SNP BLAST are also avoided. In conclusion, the SNP ID-info provides a visualized SNP ID environment for multiple inputs and assists systematic SNP association studies. The server and user manual are available at http://bio.kuas.edu.tw/snpid-info.

  9. See Also:physica status solidi (a)physica status solidi (c)Copyright © 2004 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim

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    NASA Astrophysics Data System (ADS)

    Naumov, A. V.; Vainer, Yu. G.; Bauer, M.; Kador, L.

    2004-12-01

    Editor's Choice of this issue of physica status solidi (b) is the article [1] by Andrei V. Naumov et al. This paper is Part II (Part I see [2]) of a study on elementary excitations in glasses, presented at the 11th International Conference on Phonon Scattering in Condensed Matter, St. Petersburg, 25-30 July 2004. For his outstanding talk, Naumov received the new physica status solidi Young Researcher Award which was bestowed for the first time at this conference.The cover picture is a sketch of a glass with a single impurity molecule and one hypothetical quasi-localized vibrational mode. The broadening and shift of the chromophore spectral line are caused by the interaction with this mode.Andrei V. Naumov is senior scientific researcher and deputy head of the Molecular Spectroscopy Department of the Institute of Spectroscopy, Troitsk. His main research interests are experimental and theoretical studies of low-temperature dynamics of amorphous solids (glasses, polymers etc.) via high resolution laser selective spectroscopy techniques.The second Editor's Choice is an article by E. A. Eliseev and M. D. Glinchuk [3]. Eugene A. Eliseev is scientific researcher at the Frantsevich Institute for Problems of Materials Science of the Ukrainian National Academy of Sciences, Kiev. His research areas are the theory of size and correlation effects in ferroelectric materials as well as modelling of disordered ferroelectrics properties.

  13. 76 FR 50969 - Further Inquiry Into Four Issues in the Universal Service Lifeline/Link Up Reform and...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-08-17

    ... FR 24121, May 1, 1998. Electronic Filers: Comments may be filed electronically using the Internet by... Rulemaking (NPRM or 2011 Lifeline and Link Up NPRM), 76 FR 16482, March 23, 2011. The NPRM sought public... establish eligibility for the Low Income Home Energy Assistance Program (LIHEAP) or the definition used...

  14. Employer-Led Organizations and Skill Supply Chains: Linking Worker Advancement with the Skill Needs of Employers. Issue Brief.

    ERIC Educational Resources Information Center

    Mills, Jack; Prince, Heath

    Skill supply chains apply a chain strategy to human resources to make the labor market more efficient. They link the multiple skill levels in a given labor market within a network of recruitment pathways for employers and advancement pathways for workers. Skill supply chains are based on employers' actual skill needs and on the principle that…

  15. Anti-ids in allergy: timeliness of a classic concept.

    PubMed

    Wallmann, Julia; Pali-Schöll, Isabella; Jensen-Jarolim, Erika

    2010-06-01

    Anti-idiotypic antibodies (anti-ids) are part of natural immune responses with regulatory capacity. Their effect on an antigen-specific, so-called Ab1 antibody response, is dependent on 1) the original antigen, which they mirror, being Ab2 antibodies, and 2) their isotype. In the case of IgE-mediated allergy, natural anti-ids against allergen-specific IgE represent internal images of allergen molecules. A key biologic feature of allergens is that they can crosslink IgE, expressed by B-lymphocytes or passively bound via high affinity receptors to effector cells, which renders cellular activation. Therefore, the IgE cross linking capability of anti-ids determines whether they dampen or enhance immediate-type hypersensitivity. Correspondingly to classic antiallergen blocking IgG antibodies, anti-ids may also interact with inhibitory FcγRIIb receptors and, thereby, down-regulate T(H)2-type inflammation. Anti-ids and other B-cell epitope mimetics, like mimotopes and DARPins, represent antigen surrogates, which can be used for vaccination. Intriguingly, they may induce antibody responses without activating potentially proinflammatory, antiallergen T-lymphocytes. Taken together, collective evidence suggests that anti-ids, although representing immunologic classics, are a timeless concept in allergology.

  16. Communities of practice: the missing link for knowledge management on implementation issues in low-income countries?

    PubMed

    Meessen, Bruno; Kouanda, Seni; Musango, Laurent; Richard, Fabienne; Ridde, Valéry; Soucat, Agnès

    2011-08-01

    The implementation of policies remains a huge challenge in many low-income countries. Several factors play a role in this, but improper management of existing knowledge is no doubt a major issue. In this article, we argue that new platforms should be created that gather all stakeholders who hold pieces of relevant knowledge for successful policies. To build our case, we capitalize on our experience in our domain of practice, health care financing in sub-Saharan Africa. We recently adopted a community of practice strategy in the region. More in general, we consider these platforms as the way forward for knowledge management of implementation issues. PMID:21564426

  17. Communities of practice: the missing link for knowledge management on implementation issues in low-income countries?

    PubMed

    Meessen, Bruno; Kouanda, Seni; Musango, Laurent; Richard, Fabienne; Ridde, Valéry; Soucat, Agnès

    2011-08-01

    The implementation of policies remains a huge challenge in many low-income countries. Several factors play a role in this, but improper management of existing knowledge is no doubt a major issue. In this article, we argue that new platforms should be created that gather all stakeholders who hold pieces of relevant knowledge for successful policies. To build our case, we capitalize on our experience in our domain of practice, health care financing in sub-Saharan Africa. We recently adopted a community of practice strategy in the region. More in general, we consider these platforms as the way forward for knowledge management of implementation issues.

  18. Linking Housing and School Integration Policy: What Federal, State and Local Governments Can Do. Issue Brief No. 5

    ERIC Educational Resources Information Center

    Tegeler, Philip

    2015-01-01

    This Issue Brief states that, in spite of the obvious "reciprocal relationship" between housing and school policy, government housing and education agencies have rarely collaborated to promote the common goals of racial and economic integration. Recent efforts to promote collaboration among housing and school agencies have focused on…

  19. K-feldspar megacrysts growth and their link to the granitic mush: insight from high precision U-Pb dates (ID-TIMS) and trace elements (TIMS-TEA) on zircon

    NASA Astrophysics Data System (ADS)

    Barboni, M.; Schoene, B.

    2012-12-01

    K-feldspar megacrysts are common in granitic to granodioritic rocks though their origin and evolution is still poorly understood. Their occurrence seems to reflect a low nucleation rate relative to growth rate at low degrees of supersaturation. Though K-feldspar has been argued to be one of the last minerals to saturate in felsic magma, crystal mush may still contain 60-70% liquid at the initiation of crystallization. Therefore, abundant liquid might be available for development of large crystals early in the magma cooling process. The recent recognition that many granitoid plutons are built incrementally by many magma injections open new perspectives for K-feldspar megacrysts formation. Repeated replenishment, reheating and reinvigoration of the mush might play a significant role in making and preserving K-feldspar megacrysts. The Miocene Elba island intrusives (Italy) are famous for having some of the largest K-feldspar megacrysts ever found (up to 25 cm.). This young plutonic body emplaced incrementally at shallow crustal level (ca. 3km) and displays extensive evidence of interactions between magmas of contrasting compositions. We have sampled three different families of K-feldspar megacrysts occurring in different host rock and have combined U/Pb high precision dates (ID-TIMS) with trace elements (TIMS-TEA) and Hf isotopes of zircons that are included within the K-feldspar megacrysts and compared them to zircons from the host rock. Ages of zircon included in the megacrysts could document whether the crystals grew early in the magmatic system (inclusions older than the host rock) or late during the cooling history (similar ages within inclusions and host rock). Ti-in zircon thermometer combined with trace-elements signature could record slight differences between megacrysts and host rock zircons, documenting the development of the megacrysts in comparison with the one of the host granite. Strong field evidence support a magmatic/ phenocrystic origin for the Elba

  20. Multimodal biometrics for identity documents (MBioID).

    PubMed

    Dessimoz, Damien; Richiardi, Jonas; Champod, Christophe; Drygajlo, Andrzej

    2007-04-11

    The MBioID initiative has been set up to address the following germane question: What and how biometric technologies could be deployed in identity documents in the foreseeable future? This research effort proposes to look at current and future practices and systems of establishing and using biometric identity documents (IDs) and evaluate their effectiveness in large-scale developments. The first objective of the MBioID project is to present a review document establishing the current state-of-the-art related to the use of multimodal biometrics in an IDs application. This research report gives the main definitions, properties and the framework of use related to biometrics, an overview of the main standards developed in the biometric industry and standardisation organisations to ensure interoperability, as well as some of the legal framework and the issues associated to biometrics such as privacy and personal data protection. The state-of-the-art in terms of technological development is also summarised for a range of single biometric modalities (2D and 3D face, fingerprint, iris, on-line signature and speech), chosen according to ICAO recommendations and availabilities, and for various multimodal approaches. This paper gives a summary of the main elements of that report. The second objective of the MBioID project is to propose relevant acquisition and evaluation protocols for a large-scale deployment of biometric IDs. Combined with the protocols, a multimodal database will be acquired in a realistic way, in order to be as close as possible to a real biometric IDs deployment. In this paper, the issues and solutions related to the acquisition setup are briefly presented.

  21. A comprehensive protein-centric ID mapping service for molecular data integration

    PubMed Central

    Huang, Hongzhan; Suzek, Baris E.; Mazumder, Raja; Zhang, Jian; Chen, Yongxing; Wu, Cathy H.

    2011-01-01

    Motivation: Identifier (ID) mapping establishes links between various biological databases and is an essential first step for molecular data integration and functional annotation. ID mapping allows diverse molecular data on genes and proteins to be combined and mapped to functional pathways and ontologies. We have developed comprehensive protein-centric ID mapping services providing mappings for 90 IDs derived from databases on genes, proteins, pathways, diseases, structures, protein families, protein interaction, literature, ontologies, etc. The services are widely used and have been regularly updated since 2006. Availability: www.uniprot.org/mappingandproteininformation-resource.org/pirwww/search/idmapping.shtml Contact: huang@dbi.udel.edu PMID:21478197

  22. Threshold extended ID3 algorithm

    NASA Astrophysics Data System (ADS)

    Kumar, A. B. Rajesh; Ramesh, C. Phani; Madhusudhan, E.; Padmavathamma, M.

    2012-04-01

    Information exchange over insecure networks needs to provide authentication and confidentiality to the database in significant problem in datamining. In this paper we propose a novel authenticated multiparty ID3 Algorithm used to construct multiparty secret sharing decision tree for implementation in medical transactions.

  23. The Numbers Game: Phasing in Generated ID Numbers at the University of Oregon

    ERIC Educational Resources Information Center

    Eveland, Sue

    2005-01-01

    With all the recent headlines about security breaches and information loss at financial and educational institutions, the higher education community needs to address the issue of using social security numbers as ID numbers. The University of Oregon undertook a change process to assign generated ID numbers to all records in their information…

  1. Reducing Risks to Women Linked to Shift Work, Long Work Hours, and Related Workplace Sleep and Fatigue Issues.

    PubMed

    Caruso, Claire C

    2015-10-01

    In the United States, an estimated 12% to 28% of working women are on shift work schedules, and 12% work more than 48 hours per week. Shift work and long work hours are associated with many health and safety risks, including obesity, injuries, and negative reproductive outcomes. Over time, the worker is at risk for developing a wide range of chronic diseases. These work schedules can also strain personal relationships, owing to fatigue and poor mood from sleep deprivation and reduced quality time to spend with family and friends. Worker errors from fatigue can lead to reduced quality of goods and services, negatively impacting the employer. In addition, mistakes by fatigued workers can have far-reaching negative effects on the community, ranging from medical care errors to motor vehicle crashes and industrial disasters that endanger others. To reduce the many risks that are linked to these demanding work hours, the National Institute for Occupational Safety and Health (NIOSH) conducts research, develops guidance and authoritative recommendations, and translates and disseminates scientific information to protect workers, their families, employers, and the community. The key message to reduce these risks is making sleep a priority in the employer's systems for organizing work and in the worker's personal life. The NIOSH website has freely available online training programs with suggestions for workers and their managers to help them better cope with this workplace hazard. PMID:26334800

  2. Reducing Risks to Women Linked to Shift Work, Long Work Hours, and Related Workplace Sleep and Fatigue Issues.

    PubMed

    Caruso, Claire C

    2015-10-01

    In the United States, an estimated 12% to 28% of working women are on shift work schedules, and 12% work more than 48 hours per week. Shift work and long work hours are associated with many health and safety risks, including obesity, injuries, and negative reproductive outcomes. Over time, the worker is at risk for developing a wide range of chronic diseases. These work schedules can also strain personal relationships, owing to fatigue and poor mood from sleep deprivation and reduced quality time to spend with family and friends. Worker errors from fatigue can lead to reduced quality of goods and services, negatively impacting the employer. In addition, mistakes by fatigued workers can have far-reaching negative effects on the community, ranging from medical care errors to motor vehicle crashes and industrial disasters that endanger others. To reduce the many risks that are linked to these demanding work hours, the National Institute for Occupational Safety and Health (NIOSH) conducts research, develops guidance and authoritative recommendations, and translates and disseminates scientific information to protect workers, their families, employers, and the community. The key message to reduce these risks is making sleep a priority in the employer's systems for organizing work and in the worker's personal life. The NIOSH website has freely available online training programs with suggestions for workers and their managers to help them better cope with this workplace hazard.

  3. IDS contribution to ITRF2008

    NASA Astrophysics Data System (ADS)

    Valette, Jean-Jacques; Lemoine, Frank G.; Ferrage, Pascale; Yaya, Philippe; Altamimi, Zuheir; Willis, Pascal; Soudarin, Laurent

    2010-12-01

    For the first time, the International DORIS Service (IDS) has produced a technique level combination based on the contributions of seven analysis centers (ACs), including the European Space Operations Center (ESOC), Geodetic Observatory Pecny (GOP), Geoscience Australia (GAU), the NASA Goddard Space Flight Center (GSFC), the Institut Géographique National (IGN), the Institute of Astronomy, Russian Academy of Sciences (INASAN, named as INA), and CNES/CLS (named as LCA). The ACs used five different software packages to process the DORIS data from 1992 to 2008, including NAPEOS (ESA), Bernese (GOP), GEODYN (GAU, GSC), GIPSY/OASIS (INA), and GINS (LCA). The data from seven DORIS satellites, TOPEX/Poseidon, SPOT-2, SPOT-3, SPOT-4, SPOT-5, Envisat and Jason-1 were processed and all the analysis centers produced weekly SINEX files in either variance-covariance or normal equation format. The processing by the analysis centers used the latest GRACE-derived gravity models, forward modelling of atmospheric gravity, updates to the radiation pressure modelling to improve the DORIS geocenter solutions, denser parameterization of empirically determined drag coefficients to improve station and EOP solutions, especially near the solar maximum in 2001-2002, updated troposphere mapping functions, and an ITRF2005-derived station set for orbit determination, DPOD2005. The CATREF software was used to process the weekly AC solutions, and produce three iterations of an IDS global weekly combination. Between the development of the initial solution IDS-1, and the final solution, IDS-3, the ACs improved their analysis strategies and submitted updated solutions to eliminate troposphere-derived biases in the solution scale, to reduce drag-related degradations in station positioning, and to refine the estimation strategy to improve the combination geocenter solution. An analysis of the frequency content of the individual AC geocenter and scale solutions was used as the basis to define the

  4. One ID Card for the Entire Campus.

    ERIC Educational Resources Information Center

    Ridenour, David P.; Ferguson, Linda M.

    1986-01-01

    The implementation by Indiana State University of a machine-readable photo ID system for their food services prompted an investigation into the available alternatives and requirements for a more efficient all-University ID card system. The new ID system is described. (AUTHOR/MLW)

  5. IDS-C and IDS-sr: psychometric properties in depressed in-patients.

    PubMed

    Corruble, E; Legrand, J M; Duret, C; Charles, G; Guelfi, J D

    1999-12-01

    Sixty-eight depressed in-patients were assessed at admission (DO), and after 5 days (D5), ten days (D10) and 28 days (D28) of antidepressant treatment, with the Inventory for Depressive Symptomatology-Clinician (IDS-C) and the Inventory for Depressive Symptomatology-Self-Rated (IDS-SR) (Rush et al., 1986), the Montgomery and Asberg Depression Rating Scale (MADRS) (Montgomery and Asberg, 1979) and the depression factor of the Symptom Check List (SCL-90R) (Derogatis, 1977), in order to assess IDS-C and IDS-SR psychometric properties in depressed in-patients and to compare IDS-C to MADRS and IDS-SR to the SCL-90R depression factor. Most of the IDS-C and IDS-SR items were significantly correlated to the final score and the Cronbach alpha coefficients were high (0.75 for the IDS-C and 0.79 for the IDS-SR). Principal Component Analyses (PCA) showed three factors for both IDS-C and IDS-SR: 'depression', 'anxiety/arousal' and 'sleep/appetite'. These results suggest satisfactory internal consistency of IDS-C and IDS-SR. Concurrent validity of the IDS-C with the MADRS was high (r = 0.81), as well as concurrent validity of the IDS-SR with the SCL-90R depression factor (r = 0.84). Concerning sensitivity to change, the four scales were able to discriminate between different levels of severity of depression. Moreover, considering paired t-tests on score changes, IDS-C sensitivity to change may be higher than MADRS sensitivity to change, this phenomenon being related to the number of items and degrees but not to the item contents. Contrary to IDS-C and MADRS, IDS-SR and SCL-90R depression factor were not different in terms of sensitivity to change. Finally, psychometric properties of IDS-C and IDS-SR in depressed in-patients are satisfactory and close to those obtained in depressed out-patients. The high sensitivity to change of the IDS-C may be an advantage for this scale as compared to the MADRS, especially in antidepressant drug trials.

  6. Id proteins: small molecules, mighty regulators.

    PubMed

    Ling, Flora; Kang, Bin; Sun, Xiao-Hong

    2014-01-01

    The family of inhibitor of differentiation (Id) proteins is a group of evolutionarily conserved molecules, which play important regulatory roles in organisms ranging from Drosophila to humans. Id proteins are small polypeptides harboring a helix-loop-helix (HLH) motif, which are best known to mediate dimerization with other basic HLH proteins, primarily E proteins. Because Id proteins do not possess the basic amino acids adjacent to the HLH motif necessary for DNA binding, Id proteins inhibit the function of E protein homodimers, as well as heterodimers between E proteins and tissue-specific bHLH proteins. However, Id proteins have also been shown to have E protein-independent functions. The Id genes are broadly but differentially expressed in a variety of cell types. Transcription of the Id genes is controlled by transcription factors such as C/EBPβ and Egr as well as by signaling pathways triggered by different stimuli, which include bone morphogenic proteins, cytokines, and ligands of T cell receptors. In general, Id proteins are capable of inhibiting the differentiation of progenitors of different cell types, promoting cell-cycle progression, delaying cellular senescence, and facilitating cell migration. These properties of Id proteins enable them to play significant roles in stem cell maintenance, vasculogenesis, tumorigenesis and metastasis, the development of the immune system, and energy metabolism. In this review, we intend to highlight the current understanding of the function of Id proteins and discuss gaps in our knowledge about the mechanisms whereby Id proteins exert their diverse effects in multiple cellular processes.

  7. Identity crisis? The need for systematic gene IDs.

    PubMed

    Parsons, Marilyn; Myler, Peter J; Berriman, Matthew; Roos, David S; Stuart, Kenneth D

    2011-05-01

    Recent years have seen an explosion in the availability of protozoan pathogen genome sequences. Although data regarding the underlying genome sequence remain relatively stable after the initial draft, understanding of specific gene function is increasing rapidly. This dichotomy is reflected in the relative stability of systematic gene identifiers (SysIDs(*)) in genome sequence databases, as compared to evolving and/or conflicting gene and gene product names. GenBank/EMBL/DDBJ accession numbers are important, but most protozoan parasite researchers use organism-based databases such as EuPathDB or GeneDB as their immediate resource for gene-based information because they not only provide sequence information but also functional information and links to references. Reference to SysIDs therefore provides a valuable bridge to this repository of information.

  8. R&D100: IC ID

    SciTech Connect

    Hamlet, Jason; Pierson, Lyndon; Bauer, Todd

    2015-11-19

    Supply chain security to detect, deter, and prevent the counterfeiting of networked and stand-alone integrated circuits (ICs) is critical to cyber security. Sandia National Laboratory researchers have developed IC ID to leverage Physically Unclonable Functions (PUFs) and strong cryptographic authentication to create a unique fingerprint for each integrated circuit. IC ID assures the authenticity of ICs to prevent tampering or malicious substitution.

  9. Tailoring a corporate compliance program for an IDS.

    PubMed

    Berkey, M F

    1999-01-01

    Integrated delivery systems (IDSs) face a particular challenge in implementing a corporate compliance program--that of ensuring the program addresses issues of compliance with payment rules and regulations consistently across every component of the health system. To successfully implement an effective compliance program, the various components of the IDS first must understand what such a program is intended to accomplish, and any internal resistance to developing the program must be overcome. The IDS then can undertake identifying and assessing areas of the health system at risk of being in noncompliance, designing and implementing the program, auditing claims processing and cost reporting throughout the health system to ensure systemwide compliance, and maintaining the program through ongoing monitoring and training. PMID:10339165

  10. Tailoring a corporate compliance program for an IDS.

    PubMed

    Berkey, M F

    1999-01-01

    Integrated delivery systems (IDSs) face a particular challenge in implementing a corporate compliance program--that of ensuring the program addresses issues of compliance with payment rules and regulations consistently across every component of the health system. To successfully implement an effective compliance program, the various components of the IDS first must understand what such a program is intended to accomplish, and any internal resistance to developing the program must be overcome. The IDS then can undertake identifying and assessing areas of the health system at risk of being in noncompliance, designing and implementing the program, auditing claims processing and cost reporting throughout the health system to ensure systemwide compliance, and maintaining the program through ongoing monitoring and training.

  11. Id3 inhibits B lymphocyte progenitor growth and survival in response to TGF-beta.

    PubMed

    Kee, B L; Rivera, R R; Murre, C

    2001-03-01

    E proteins function in many developmental processes and are essential for the formation of lymphocyte progenitors. However, it is not known whether E proteins regulate lymphocyte survival, proliferation or differentiation or how their activity is regulated during lymphocyte development. We show here a role for Id3, an inhibitor of E protein activity, in the induction of apoptosis and growth arrest. Id3 is induced in response to transforming growth factor beta (TGF-beta), a pleiotropic cytokine that inhibits the growth and survival of normal and transformed lymphocyte progenitors. In the absence of Id3, the response of lymphocyte progenitors to TGF-beta is perturbed, which indicates that Id3 is a mediator of this response. Our data show a key role for E proteins in lymphocyte survival and link the activity of E proteins, and their antagonists, to members of the TGF-beta family of cytokines.

  12. Id-1 and Id-2 genes and products as markers of epithelial cancer

    DOEpatents

    Desprez, Pierre-Yves; Campisi, Judith

    2008-09-30

    A method for detection and prognosis of breast cancer and other types of cancer. The method comprises detecting expression, if any, for both an Id-1 and an Id-2 genes, or the ratio thereof, of gene products in samples of breast tissue obtained from a patient. When expressed, Id-1 gene is a prognostic indicator that breast cancer cells are invasive and metastatic, whereas Id-2 gene is a prognostic indicator that breast cancer cells are localized and noninvasive in the breast tissue.

  13. Id-1 and Id-2 genes and products as markers of epithelial cancer

    DOEpatents

    Desprez, Pierre-Yves; Campisi, Judith

    2011-10-04

    A method for detection and prognosis of breast cancer and other types of cancer. The method comprises detecting expression, if any, for both an Id-1 and an Id-2 genes, or the ratio thereof, of gene products in samples of breast tissue obtained from a patient. When expressed, Id-1 gene is a prognostic indicator that breast cancer cells are invasive and metastatic, whereas Id-2 gene is a prognostic indicator that breast cancer cells are localized and noninvasive in the breast tissue.

  14. A CORBA-based object framework with patient identification translation and dynamic linking. Methods for exchanging patient data.

    PubMed

    Wang, C; Ohe, K

    1999-03-01

    Exchanging and integration of patient data across heterogeneous databases and institutional boundaries offers many problems. We focused on two issues: (1) how to identify identical patients between different systems and institutions while lacking universal patient identifiers; and (2) how to link patient data across heterogeneous databases and institutional boundaries. To solve these problems, we created a patient identification (ID) translation model and a dynamic linking method in the Common Object Request Broker Architecture (CORBA) environment. The algorithm for the patient ID translation is based on patient attribute matching plus computer-based human checking; the method for dynamic linking is temporal mapping. By implementing these methods into computer systems with help of the distributed object computing technology, we built a prototype of a CORBA-based object framework in which the patient ID translation and dynamic linking methods were embedded. Our experiments with a Web-based user interface using the object framework and dynamic linking-through the object framework were successful. These methods are important for exchanging and integrating patient data across heterogeneous databases and institutional boundaries.

  15. PEROXISOME PROLIFERATOR-ACTIVATED RECEPTORα (PPARα) AGONISTS DIFFERENTIALLY REGULATE INHIBITOR OF DNA BINDING (ID2) EXPRESSION IN RODENTS AND HUMAN CELLS

    EPA Science Inventory

    Abstract Inhibitor of DNA binding (Id2) is a member of the helix-loop-helix (HLH) transcription factor family whose members play important roles in cell differentiation and proliferation. Id2 has been linked to the development of cardiovascular diseases since thiazolidinediones,...

  16. TGFβ-Id1 Signaling Opposes Twist1 and Promotes Metastatic Colonization Via a Mesenchymal-to-Epithelial Transition

    PubMed Central

    Stankic, Marko; Pavlovic, Svetlana; Chin, Yvette; Brogi, Edi; Padua, David; Norton, Larry; Massague, Joan; Benezra, Robert

    2014-01-01

    SUMMARY ID genes are required for breast cancer colonization of the lungs, but the mechanism remains poorly understood. Here, we show that Id1 expression induces a stem-like phenotype in breast cancer cells, while retaining epithelial properties, contrary to the notion that cancer stem-like properties are inextricably linked to the mesenchymal state. During metastatic colonization, Id1 induces a mesenchymal-to-epithelial transition (MET), specifically in cells whose mesenchymal state is dependent on the Id1 target protein Twist1 but not at the primary site, where this state is controlled by the zinc-finger protein Snail1. Knockdown of Id expression in metastasizing cells prevents MET and dramatically reduces lung colonization. Furthermore, Id1 is induced by TGFβ only in cells that have first undergone EMT, demonstrating that EMT is a pre-requisite for subsequent Id1-induced MET during lung colonization. Collectively, these studies underscore the importance of Id-mediated phenotypic switching during distinct stages of breast cancer metastasis. PMID:24332369

  17. Commercial Web Site Links.

    ERIC Educational Resources Information Center

    Thelwall, Mike

    2001-01-01

    Discusses business use of the Web and related search engine design issues as well as research on general and academic links before reporting on a survey of the links published by a collection of business Web sites. Results indicate around 66% of Web sites do carry external links, most of which are targeted at a specific purpose, but about 17%…

  18. Examining the Links between Grade 12 Mathematics and Remedial Courses in Nevada Public Colleges and Universities. Issues & Answers. REL 2008-No. 058

    ERIC Educational Resources Information Center

    Fong, Anthony B.; Huang, Min; Goel, Aditi M.

    2008-01-01

    This study examines the links between Nevada's grade 12 mathematics courses and remedial mathematics courses in Nevada's public colleges and universities. It analyzes remediation rates by students' highest grade 12 mathematics course level and mathematics grade point average and by various student and school characteristics. The study was guided…

  19. A Cognitive Model of the Effects of Linking Political Referenda to Salient Social Issues: A Lottery Will Fund Education, But Casinos Will Cause Crime?

    ERIC Educational Resources Information Center

    Ferguson, M. A.; And Others

    Contrasting a party identification model with a rational decision making model, a cognitive model predicts voter intent on two referenda: (1) an amendment to permit a state lottery; and (2) an amendment to permit residents of each county to vote on whether to permit casino gambling. Supporters of the lottery amendment attempt to strongly link it…

  20. Theoretical Issues

    SciTech Connect

    Marc Vanderhaeghen

    2007-04-01

    The theoretical issues in the interpretation of the precision measurements of the nucleon-to-Delta transition by means of electromagnetic probes are highlighted. The results of these measurements are confronted with the state-of-the-art calculations based on chiral effective-field theories (EFT), lattice QCD, large-Nc relations, perturbative QCD, and QCD-inspired models. The link of the nucleon-to-Delta form factors to generalized parton distributions (GPDs) is also discussed.

  1. 32 CFR 161.7 - ID card life-cycle procedures.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... of DoD ID cards. The use of the CAC, as the DoD Federal personal identity verification (PIV) card, is... 201-1, “Personal Identity Verification (PIV) of Federal Employees and Contractors” (available at http... Memorandum, “Final Credentialing Standards for Issuing Personal Identity Verification Cards under...

  2. Selection of starter cultures for idli batter fermentation and their effect on quality of idlis.

    PubMed

    Sridevi, J; Halami, Prakash M; Vijayendra, S V N

    2010-10-01

    Idli batter samples were prepared using lactic starter cultures like Pediococcus pentosaceus (Pp), Enterococcus faecium MTCC 5153 (Ef), Ent. faecium (IB2 Ef-IB2), individually, along with the yeast culture, Candida versatilis (Cv). Idli batter prepared using Ef and Ef-IB2 cultures gave better results, when evaluated for the rise in batter volume (80 ml), level of CO2 production (23.8%), titratable acidity 2.4-3.5% (lactic acid) and pH 4.3-4.4. Storage stability of batter made with selected starter cultures was determined by analyzing the idlis prepared using the batter stored for 1 and 5 days for texture, nutrient composition and sensory quality. Slight variations in the results were seen among the idlis of different combination of cultures, whereas these results are better than that of the idlis made using naturally fermented idli batter. Sensory profile of idlis prepared using starter cultures had a higher score (3.9-4.4) compared to the control (3.6) for overall acceptability.

  3. Selection of starter cultures for idli batter fermentation and their effect on quality of idlis.

    PubMed

    Sridevi, J; Halami, Prakash M; Vijayendra, S V N

    2010-10-01

    Idli batter samples were prepared using lactic starter cultures like Pediococcus pentosaceus (Pp), Enterococcus faecium MTCC 5153 (Ef), Ent. faecium (IB2 Ef-IB2), individually, along with the yeast culture, Candida versatilis (Cv). Idli batter prepared using Ef and Ef-IB2 cultures gave better results, when evaluated for the rise in batter volume (80 ml), level of CO2 production (23.8%), titratable acidity 2.4-3.5% (lactic acid) and pH 4.3-4.4. Storage stability of batter made with selected starter cultures was determined by analyzing the idlis prepared using the batter stored for 1 and 5 days for texture, nutrient composition and sensory quality. Slight variations in the results were seen among the idlis of different combination of cultures, whereas these results are better than that of the idlis made using naturally fermented idli batter. Sensory profile of idlis prepared using starter cultures had a higher score (3.9-4.4) compared to the control (3.6) for overall acceptability. PMID:23572685

  4. What Is "Real-World" ID Anyway?

    ERIC Educational Resources Information Center

    Hardre, Patricia L.

    2013-01-01

    The term "real-world ID" is commonly heard at professional conferences, but its meaning is assumed rather than defined. Unless we examine the meanings and implications of rhetoric of the field, we risk error in presenting ourselves, and we risk derailing the progress of our profession. This article examines the term "real world" as applied to…

  5. 76 FR 31388 - Idaho Disaster #ID-00014

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-05-31

    ... From the Federal Register Online via the Government Publishing Office SMALL BUSINESS ADMINISTRATION Idaho Disaster ID-00014 AGENCY: U.S. Small Business Administration. ACTION: Notice. SUMMARY: This is a Notice of the Presidential declaration of a major disaster for Public Assistance Only for...

  6. 75 FR 45682 - Idaho Disaster #ID-00010

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-08-03

    ... From the Federal Register Online via the Government Publishing Office SMALL BUSINESS ADMINISTRATION Idaho Disaster ID-00010 AGENCY: U.S. Small Business Administration. ACTION: Notice. SUMMARY: This is a Notice of the Presidential declaration of a major disaster for Public Assistance Only for...

  7. Characterization of Inhibitor of differentiation (Id) proteins in human cornea.

    PubMed

    Mohan, Rajiv R; Morgan, Brandie R; Anumanthan, Govindaraj; Sharma, Ajay; Chaurasia, Shyam S; Rieger, Frank G

    2016-05-01

    Inhibitor of differentiation (Id) proteins are DNA-binding transcription factors involved in cellular proliferation, migration, inflammation, angiogenesis and fibrosis. However, their expression and role in the cornea is unknown. The present study was undertaken to characterize the expression of Id proteins and their interactions with the pro-fibrotic cytokine Transforming Growth Factor β1 (TGFβ1) and anti-fibrotic cytokine, bone morphogenic protein 7 (BMP7) in human cornea. Human donor corneas procured from Eye Bank were used. Id proteins were localized in human corneal sections using immunofluorescence. Primary cultures of human corneal fibroblasts (HCF) were established and treated with either TGFβ1 (5 ng/ml) or BMP7 (10 ng/ml) for 24 h in serum free medium. Expression of Id's in response to TGFβ1, BMP7 and TGFβ1 + BMP7 was analyzed by quantitative real time PCR (qRT-PCR) and western blot analysis. Id1 and Id2 proteins were ubiquitously expressed in the epithelial cells and stromal keratocytes in human cornea. The Id1 was localized to the basal epithelial cells as seen by immunohistochemistry. HCF expressed all known mammalian Id genes (Id1-Id4). In addition, Id1 and Id2 are selectively expressed in HCF. Treatment of human recombinant TGFβ1 (5 ng/ml) to serum-starved HCF showed a significant increase in Id genes (Id1, Id2 and Id4) at 2 h time point compared to BMP7 treatment, which showed time dependent increase in the expression of Id1-Id3 at 24-48 h. Combined treatment with TGFβ1 + BMP7 to HCF showed a significant increase in Id1 transcript and an increasing trend in Id3 and Id4 expression. The results of this study suggest that Id family of genes (Id1-Id4) are localized in the human cornea and expressed in the corneal fibroblasts. Also, Id's were differentially regulated with TGFβ1 and/or BMP7 in a time dependent manner and might serve as a therapeutic target in corneal fibrosis.

  8. Enigmatic in vivo iduronate-2-sulfatase (IDS) mutant transcript correction to wild-type in Hunter syndrome.

    PubMed

    Lualdi, Susanna; Tappino, Barbara; Di Duca, Marco; Dardis, Andrea; Anderson, Christopher J; Biassoni, Roberto; Thompson, Peter W; Corsolini, Fabio; Di Rocco, Maja; Bembi, Bruno; Regis, Stefano; Cooper, David N; Filocamo, Mirella

    2010-04-01

    Sequence analysis of the X-linked iduronate-2-sulfatase (IDS) gene in two Hunter syndrome patients revealed a lack of concordance between IDS genomic DNA and cDNA. These individuals were found to be hemizygous respectively for a nonsense mutation [c.22C>T;p.R8X] and a frameshift micro-insertion [c.10insT;p.P4Sfs] in their genomic DNA. However, both wild-type and mutant IDS sequences were evident upon cDNA analysis. Similar discrepant results were also obtained in a third unrelated patient carrying the same p.R8X mutation. Since both p.R8X mutations were inherited from carrier mothers, somatic mosaicism could be excluded. Although the presence of wild-type IDSmRNA-transcripts was confirmed in all three patients by restriction enzyme digestion, clone sequencing, pyrosequencing and single nucleotide primer extension (SNuPE), no wild-type IDS genomic sequence was detectable. The relative abundance of wild-type and mutation-bearing IDS-transcripts in different tissues was quantified by SNuPE. Although IDS transcript levels, as measured by real-time PCR, were reduced (51-71% normal) in these patients, some wild-type IDS protein was detectable by western blotting. Various possible explanations for these unprecedented findings (e.g. accidental contamination, artefactual in vitro nucleotide misincorporation, malsegregation of an extra maternal X-chromosome) were explored and experimentally excluded. PCR-based discriminant assay and segregation analysis of a linked IDS polymorphism (rs1141608) also served to exclude the presence of IDS cDNA derived from the maternal wild-type chromosome. Although it remains to be formally demonstrated by direct experimentation, the intriguing possibility arises that we have observed the in vivo correction of heritable gene lesions at the RNA level operating via a correction mechanism akin to RNA-editing. (c) 2010 Wiley-Liss, Inc.

  9. On estimation and identifiability issues of sex-linked inheritance with a case study of pigmentation in Swiss barn owl (Tyto alba).

    PubMed

    Larsen, Camilla T; Holand, Anna M; Jensen, Henrik; Steinsland, Ingelin; Roulin, Alexandre

    2014-05-01

    Genetic evaluation using animal models or pedigree-based models generally assume only autosomal inheritance. Bayesian animal models provide a flexible framework for genetic evaluation, and we show how the model readily can accommodate situations where the trait of interest is influenced by both autosomal and sex-linked inheritance. This allows for simultaneous calculation of autosomal and sex-chromosomal additive genetic effects. Inferences were performed using integrated nested Laplace approximations (INLA), a nonsampling-based Bayesian inference methodology. We provide a detailed description of how to calculate the inverse of the X- or Z-chromosomal additive genetic relationship matrix, needed for inference. The case study of eumelanic spot diameter in a Swiss barn owl (Tyto alba) population shows that this trait is substantially influenced by variation in genes on the Z-chromosome ([Formula: see text] and [Formula: see text]). Further, a simulation study for this study system shows that the animal model accounting for both autosomal and sex-chromosome-linked inheritance is identifiable, that is, the two effects can be distinguished, and provides accurate inference on the variance components. PMID:24967075

  10. On estimation and identifiability issues of sex-linked inheritance with a case study of pigmentation in Swiss barn owl (Tyto alba).

    PubMed

    Larsen, Camilla T; Holand, Anna M; Jensen, Henrik; Steinsland, Ingelin; Roulin, Alexandre

    2014-05-01

    Genetic evaluation using animal models or pedigree-based models generally assume only autosomal inheritance. Bayesian animal models provide a flexible framework for genetic evaluation, and we show how the model readily can accommodate situations where the trait of interest is influenced by both autosomal and sex-linked inheritance. This allows for simultaneous calculation of autosomal and sex-chromosomal additive genetic effects. Inferences were performed using integrated nested Laplace approximations (INLA), a nonsampling-based Bayesian inference methodology. We provide a detailed description of how to calculate the inverse of the X- or Z-chromosomal additive genetic relationship matrix, needed for inference. The case study of eumelanic spot diameter in a Swiss barn owl (Tyto alba) population shows that this trait is substantially influenced by variation in genes on the Z-chromosome ([Formula: see text] and [Formula: see text]). Further, a simulation study for this study system shows that the animal model accounting for both autosomal and sex-chromosome-linked inheritance is identifiable, that is, the two effects can be distinguished, and provides accurate inference on the variance components.

  11. On estimation and identifiability issues of sex-linked inheritance with a case study of pigmentation in Swiss barn owl (Tyto alba)

    PubMed Central

    Larsen, Camilla T; Holand, Anna M; Jensen, Henrik; Steinsland, Ingelin; Roulin, Alexandre

    2014-01-01

    Genetic evaluation using animal models or pedigree-based models generally assume only autosomal inheritance. Bayesian animal models provide a flexible framework for genetic evaluation, and we show how the model readily can accommodate situations where the trait of interest is influenced by both autosomal and sex-linked inheritance. This allows for simultaneous calculation of autosomal and sex-chromosomal additive genetic effects. Inferences were performed using integrated nested Laplace approximations (INLA), a nonsampling-based Bayesian inference methodology. We provide a detailed description of how to calculate the inverse of the X- or Z-chromosomal additive genetic relationship matrix, needed for inference. The case study of eumelanic spot diameter in a Swiss barn owl (Tyto alba) population shows that this trait is substantially influenced by variation in genes on the Z-chromosome ( and ). Further, a simulation study for this study system shows that the animal model accounting for both autosomal and sex-chromosome-linked inheritance is identifiable, that is, the two effects can be distinguished, and provides accurate inference on the variance components. PMID:24967075

  12. Type II arginine methyltransferase PRMT5 regulates gene expression of inhibitors of differentiation/DNA binding Id2 and Id4 during glial cell differentiation.

    PubMed

    Huang, Jinghan; Vogel, Gillian; Yu, Zhenbao; Almazan, Guillermina; Richard, Stéphane

    2011-12-30

    PRMT5 is a type II protein arginine methyltranferase that catalyzes monomethylation and symmetric dimethylation of arginine residues. PRMT5 is functionally involved in a variety of biological processes including embryo development and circadian clock regulation. However, the role of PRMT5 in oligodendrocyte differentiation and central nervous system myelination is unknown. Here we show that PRMT5 expression gradually increases throughout postnatal brain development, coinciding with the period of active myelination. PRMT5 expression was observed in neurons, astrocytes, and oligodendrocytes. siRNA-mediated depletion of PRMT5 in mouse primary oligodendrocyte progenitor cells abrogated oligodendrocyte differentiation. In addition, the PRMT5-depleted oligodendrocyte progenitor and C6 glioma cells expressed high levels of the inhibitors of differentiation/DNA binding, Id2 and Id4, known repressors of glial cell differentiation. We observed that CpG-rich islands within the Id2 and Id4 genes were bound by PRMT5 and were hypomethylated in PRMT5-deficient cells, suggesting that PRMT5 plays a role in gene silencing during glial cell differentiation. Our findings define a role of PRMT5 in glial cell differentiation and link PRMT5 to epigenetic changes during oligodendrocyte differentiation. PMID:22041901

  13. Remembering one's ID/E-ntity: E/ID protein regulation of T cell memory.

    PubMed

    Omilusik, Kyla D; Shaw, Laura A; Goldrath, Ananda W

    2013-10-01

    Upon infection, CD8(+) T cells proliferate and differentiate into armed effector cells capable of eliminating the assaulting pathogen. Although the majority of the antigen-specific T cells will die as the immune response wanes, a few will survive indefinitely to establish the memory population and provide long-lived protection against reinfection. E protein transcription factors and their inhibitors, ID proteins, operate to balance expression of genes that control CD8(+) T cell differentiation through this process. Here, we discuss the role of ID2 and ID3 in promoting the generation and survival of effector and memory populations, particularly highlighting their reciprocal roles in shaping the CD8(+) T cell response unique to the inflammatory milieu. We further examine this coordinated control of gene expression in the context of additional transcription factors within the transcriptional network that programs CD8(+) effector and memory T cell differentiation.

  14. Linking morphodynamic response with sediment mass balance on the Colorado River in Marble Canyon: issues of scale, geomorphic setting, and sampling design

    USGS Publications Warehouse

    Grams, Paul E.; Topping, David J.; Schmidt, John C.; Hazel, Joseph E.; Kaplinski, Matt

    2013-01-01

    Measurements of morphologic change are often used to infer sediment mass balance. Such measurements may, however, result in gross errors when morphologic changes over short reaches are extrapolated to predict changes in sediment mass balance for long river segments. This issue is investigated by examination of morphologic change and sediment influx and efflux for a 100 km segment of the Colorado River in Grand Canyon, Arizona. For each of four monitoring intervals within a 7 year study period, the direction of sand-storage response within short morphologic monitoring reaches was consistent with the flux-based sand mass balance. Both budgeting methods indicate that sand storage was stable or increased during the 7 year period. Extrapolation of the morphologic measurements outside the monitoring reaches does not, however, provide a reasonable estimate of the magnitude of sand-storage change for the 100 km study area. Extrapolation results in large errors, because there is large local variation in site behavior driven by interactions between the flow and local bed topography. During the same flow regime and reach-average sediment supply, some locations accumulate sand while others evacuate sand. The interaction of local hydraulics with local channel geometry exerts more control on local morphodynamic response than sand supply over an encompassing river segment. Changes in the upstream supply of sand modify bed responses but typically do not completely offset the effect of local hydraulics. Thus, accurate sediment budgets for long river segments inferred from reach-scale morphologic measurements must incorporate the effect of local hydraulics in a sampling design or avoid extrapolation altogether.

  15. Linking morphodynamic response with sediment mass balance on the Colorado River in Marble Canyon: Issues of scale, geomorphic setting, and sampling design

    NASA Astrophysics Data System (ADS)

    Grams, Paul E.; Topping, David J.; Schmidt, John C.; Hazel, Joseph E.; Kaplinski, Matt

    2013-06-01

    Measurements of morphologic change are often used to infer sediment mass balance. Such measurements may, however, result in gross errors when morphologic changes over short reaches are extrapolated to predict changes in sediment mass balance for long river segments. This issue is investigated by examination of morphologic change and sediment influx and efflux for a 100 km segment of the Colorado River in Grand Canyon, Arizona. For each of four monitoring intervals within a 7 year study period, the direction of sand-storage response within short morphologic monitoring reaches was consistent with the flux-based sand mass balance. Both budgeting methods indicate that sand storage was stable or increased during the 7 year period. Extrapolation of the morphologic measurements outside the monitoring reaches does not, however, provide a reasonable estimate of the magnitude of sand-storage change for the 100 km study area. Extrapolation results in large errors, because there is large local variation in site behavior driven by interactions between the flow and local bed topography. During the same flow regime and reach-average sediment supply, some locations accumulate sand while others evacuate sand. The interaction of local hydraulics with local channel geometry exerts more control on local morphodynamic response than sand supply over an encompassing river segment. Changes in the upstream supply of sand modify bed responses but typically do not completely offset the effect of local hydraulics. Thus, accurate sediment budgets for long river segments inferred from reach-scale morphologic measurements must incorporate the effect of local hydraulics in a sampling design or avoid extrapolation altogether.

  16. Airless drying -- Developments since IDS'94

    SciTech Connect

    Stubbing, T.J.

    1999-09-01

    Since its introduction to IDS'94 delegates, significant progress has been made with the development of airless drying technology. The ceramic industry internationally is beginning to benefit from both the energy use and drying time reductions it achieves, while on the basis of further theoretical work carried out since 1993 other industries, including the bioenergy sector, should also soon begin to exploit its advantages. As global warming becomes a reality and oil reserves decline, superheated steam drying and gasification of biomass will contribute to the mitigation of those problems.

  17. Environmental Science and Research Foundation annual technical report to DOE-ID, January , 1995--December 31, 1995

    SciTech Connect

    1996-06-01

    The foundation conducts an environmental monitoring and surveillance program over an area covering much of the upper Snake River Plain and provide environmental education and support services related to INEL natural resource issues. Also, the foundation, with its university affiliates, conducts ecological and radioecological research on the Idaho National Environmental Research Park. This research benefits major DOE-ID programs including waste management, environmental restoration, spent nuclear fuels, and land management issues. Major accomplishments during CY1995 can be divided into five categories: environmental surveillance program, environmental education, environmental services and support, ecological risk assessment, and research benefitting the DOE-ID mission.

  18. The transcriptional regulators Id2 and Id3 control the formation of distinct memory CD8+ T cell subsets.

    PubMed

    Yang, Cliff Y; Best, J Adam; Knell, Jamie; Yang, Edward; Sheridan, Alison D; Jesionek, Adam K; Li, Haiyan S; Rivera, Richard R; Lind, Kristin Camfield; D'Cruz, Louise M; Watowich, Stephanie S; Murre, Cornelis; Goldrath, Ananda W

    2011-11-06

    During infection, naive CD8(+) T cells differentiate into effector cells, which are armed to eliminate pathogens, and memory cells, which are poised to protect against reinfection. The transcriptional program that regulates terminal differentiation into short-lived effector-memory versus long-lived memory cells is not clearly defined. Through the use of mice expressing reporters for the DNA-binding inhibitors Id2 and Id3, we identified Id3(hi) precursors of long-lived memory cells before the peak of T cell population expansion or upregulation of cell-surface receptors that indicate memory potential. Deficiency in Id2 or Id3 resulted in loss of distinct CD8(+) effector and memory populations, which demonstrated unique roles for these inhibitors of E-protein transcription factors. Furthermore, cytokines altered the expression of Id2 and Id3 differently, which provides insight into how external cues influence gene expression.

  19. To ID or Not to ID? Changes in Classification Rates of Intellectual Disability Using "DSM-5"

    ERIC Educational Resources Information Center

    Papazoglou, Aimilia; Jacobson, Lisa A.; McCabe, Marie; Kaufmann, Walter; Zabel, T. Andrew

    2014-01-01

    The "Diagnostic and Statistical Manual of Mental Disorders-Fifth Edition" ("DSM-5") diagnostic criteria for intellectual disability (ID) include a change to the definition of adaptive impairment. New criteria require impairment in one adaptive domain rather than two or more skill areas. The authors examined the diagnostic…

  20. Low Physical Fitness Levels in Older Adults with ID: Results of the HA-ID Study

    ERIC Educational Resources Information Center

    Hilgenkamp, Thessa I. M.; van Wijck, Ruud; Evenhuis, Heleen M.

    2012-01-01

    Physical fitness is as important to aging adults with ID as in the general population, but to date, the physical fitness levels of this group are unknown. Comfortable walking speed, muscle strength (grip strength), muscle endurance (30 s Chair stand) and cardiorespiratory endurance (10 m incremental shuttle walking test) were tested in a sample of…

  1. Identification of Interactions in the NMD Complex Using Proximity-Dependent Biotinylation (BioID).

    PubMed

    Schweingruber, Christoph; Soffientini, Paolo; Ruepp, Marc-David; Bachi, Angela; Mühlemann, Oliver

    2016-01-01

    Proximity-dependent trans-biotinylation by the Escherichia coli biotin ligase BirA mutant R118G (BirA*) allows stringent streptavidin affinity purification of proximal proteins. This so-called BioID method provides an alternative to the widely used co-immunoprecipitation (co-IP) to identify protein-protein interactions. Here, we used BioID, on its own and combined with co-IP, to identify proteins involved in nonsense-mediated mRNA decay (NMD), a post-transcriptional mRNA turnover pathway that targets mRNAs that fail to terminate translation properly. In particular, we expressed BirA* fused to the well characterized NMD factors UPF1, UPF2 and SMG5 and detected by liquid chromatography-coupled tandem mass spectrometry (LC-MS/MS) the streptavidin-purified biotinylated proteins. While the identified already known interactors confirmed the usefulness of BioID, we also found new potentially important interactors that have escaped previous detection by co-IP, presumably because they associate only weakly and/or very transiently with the NMD machinery. Our results suggest that SMG5 only transiently contacts the UPF1-UPF2-UPF3 complex and that it provides a physical link to the decapping complex. In addition, BioID revealed among others CRKL and EIF4A2 as putative novel transient interactors with NMD factors, but whether or not they have a function in NMD remains to be elucidated. PMID:26934103

  2. Identification of Interactions in the NMD Complex Using Proximity-Dependent Biotinylation (BioID)

    PubMed Central

    Schweingruber, Christoph; Soffientini, Paolo; Ruepp, Marc-David; Bachi, Angela; Mühlemann, Oliver

    2016-01-01

    Proximity-dependent trans-biotinylation by the Escherichia coli biotin ligase BirA mutant R118G (BirA*) allows stringent streptavidin affinity purification of proximal proteins. This so-called BioID method provides an alternative to the widely used co-immunoprecipitation (co-IP) to identify protein-protein interactions. Here, we used BioID, on its own and combined with co-IP, to identify proteins involved in nonsense-mediated mRNA decay (NMD), a post-transcriptional mRNA turnover pathway that targets mRNAs that fail to terminate translation properly. In particular, we expressed BirA* fused to the well characterized NMD factors UPF1, UPF2 and SMG5 and detected by liquid chromatography-coupled tandem mass spectrometry (LC-MS/MS) the streptavidin-purified biotinylated proteins. While the identified already known interactors confirmed the usefulness of BioID, we also found new potentially important interactors that have escaped previous detection by co-IP, presumably because they associate only weakly and/or very transiently with the NMD machinery. Our results suggest that SMG5 only transiently contacts the UPF1-UPF2-UPF3 complex and that it provides a physical link to the decapping complex. In addition, BioID revealed among others CRKL and EIF4A2 as putative novel transient interactors with NMD factors, but whether or not they have a function in NMD remains to be elucidated. PMID:26934103

  3. The link in Linking

    PubMed Central

    Caldwell, Jane C; Chiale, Pablo A; Gonzalez, Mario D; Baranchuk, Adrian

    2013-01-01

    We present 2 cases of the slow-fast form of AVNRT with initially narrow QRS complexes followed by sudden unexpected transition to persistently wide QRS complexes due to aberrant intraventricular conduction. Introduction of a properly timed extrastimulus in one case and critical oscillations in cycle length due to short-long coupling in the second case set the stage for the initial bundle branch block. However, persistence of the aberrancy pattern once the initial event abated was maintained by the "linking" phenomenon. Delayed, retrograde concealed activation from the contralateral bundle branch perpetuated the initial bundle branch block. PMID:23840106

  4. Molecular characterization of Portuguese patients with mucopolysaccharidosis type II shows evidence that the IDS gene is prone to splicing mutations.

    PubMed

    Alves, S; Mangas, M; Prata, M J; Ribeiro, G; Lopes, L; Ribeiro, H; Pinto-Basto, J; Lima, M Reis; Lacerda, L

    2006-12-01

    Mucopolysaccharidosis type II (MPS II) is an X-linked recessive lysosomal storage disease caused by a defect in the iduronate-2-sulfatase gene (IDS). Alternative splicing of the IDS gene can occur and the underlying regulatory mechanism may be rather complex. Nevertheless, little information is available on the role of variations at the IDS locus in the splicing process. Here we report that splice mutations at the IDS locus are an important source of MPS II pathogenicity, accounting for almost 56% of Portuguese cases. Among 16 unrelated Portuguese MPS II patients, 15 different mutations were identified: six intronic splice mutations (c.104-2AG, c.241-2A>G, c.241-1G>A, c.418+1G>A, c.880-8AG and c.1181-1G>C); two exonic splice mutations (c.1006G>lC and c.1122C>T); five missense mutations (D269V, D69V, D148N, R88C and P86L); one nonsense mutation (Q465Ter); one total IDS gene deletion; and one rearrangement involving a IDS gene inversion. Furthermore, nine of the 15 detected mutations affected the usual splicing pattern at the locus. Some of them are responsible for dramatic changes in the splicing mechanism. For example, the substitution mutation, c.418+1G>A, revealed the presence of an exonic sequence inside intron 3. Our study provides evidence that the IDS locus is prone to splicing mutations and that such susceptibility is particularly high in exon 3 and neighbouring regions. Consequently, mutation screening of the IDS gene cannot be restricted to gDNA examination. Unless cDNA analysis is also conducted, misclassifications as silent or missense mutations can be produced and even uncharacteristic splice-site mutations can be misinterpreted as classic splicing defects that may generate severe, unconventional splicing alterations.

  5. Transcriptional regulator Id2 mediates CD8+ T cell immunity.

    PubMed

    Cannarile, Michael A; Lind, Nicholas A; Rivera, Richard; Sheridan, Alison D; Camfield, Kristin A; Wu, Bei Bei; Cheung, Kitty P; Ding, Zhaoqing; Goldrath, Ananda W

    2006-12-01

    Transcriptional programs that initiate and sustain the proliferation, differentiation and survival of CD8(+) T cells during immune responses are not completely understood. Here we show that inhibitor of DNA binding 2 (Id2), an antagonist of E protein transcription factors, was upregulated in CD8(+) T cells during infection and that expression of Id2 was maintained in memory CD8(+) T cells. Although Id2-deficient naive CD8(+) T cells recognized antigen and proliferated normally early after infection, effector CD8(+) T cells did not accumulate because the cells were highly susceptible to apoptosis. Id2-deficient CD8(+) T cells responding to infection had changes in the expression of genes that influence survival and had altered memory formation. Our data emphasize the importance of Id2 in regulating gene expression by CD8(+) T cells and the magnitude of effector responses, suggesting a mechanism involving Id protein- and E protein-mediated survival and differentiation of mature T cells.

  6. A 3' splice site mutation of IDS gene in a Chinese family with mucopolysaccharidosis type II.

    PubMed

    Jin, Ping; Hao, Jing-Wen; Chen, Ke; Dong, Chang-Sheng; Yang, You-Bo; Mo, Zhao-Hui

    2013-10-10

    The purpose of this study was to identify the underlying genetic cause in a four generation Chinese family diagnosed with mucopolysaccharidosis type II. Peripheral blood samples were collected from family members and the iduronate-2-sulfatase (IDS) gene was analyzed by DNA sequencing. The impact of IDS mutations on mRNA transcription was determined by quantitative real-time RT-PCR (qRT-PCR) in both patients as well as in healthy control samples. In addition, RT-PCR was performed to confirm the characteristics of a found mutation located in non-canonical splicing site. A 3' splice site mutation c.880-8A>G (IVS 6-8A>G) was identified in two members of the analyzed MPS II family and sequencing of RT-PCR products showed that this mutation activates an upstream cryptic splice-site in intron 6, leads to the 7 nucleotide insertion in exon 7, which in turn results in an exon 7 frameshift introducing a premature stop codon and shorter peptide chain. In addition, qRT-PCR products from the two patients showed a reduced IDS mRNA expression (43.9% and 71.2%, respectively), when compared with the average IDS mRNA expression in healthy control samples, possibly as a result of nonsense-mediated mRNA decay. In conclusion, in this study, we have identified an IDS gene splice mutation which is associated with clinically attenuated MPS II phenotype. In addition, our study accentuates the importance of cDNA analysis in the detection of intronic mutations, since in the studies examining only gDNA, the link between genotype and phenotype may have been misinterpreted.

  7. FLOR-ID: an interactive database of flowering-time gene networks in Arabidopsis thaliana.

    PubMed

    Bouché, Frédéric; Lobet, Guillaume; Tocquin, Pierre; Périlleux, Claire

    2016-01-01

    Flowering is a hot topic in Plant Biology and important progress has been made in Arabidopsis thaliana toward unraveling the genetic networks involved. The increasing complexity and the explosion of literature however require development of new tools for information management and update. We therefore created an evolutive and interactive database of flowering time genes, named FLOR-ID (Flowering-Interactive Database), which is freely accessible at http://www.flor-id.org. The hand-curated database contains information on 306 genes and links to 1595 publications gathering the work of >4500 authors. Gene/protein functions and interactions within the flowering pathways were inferred from the analysis of related publications, included in the database and translated into interactive manually drawn snapshots. PMID:26476447

  8. FLOR-ID: an interactive database of flowering-time gene networks in Arabidopsis thaliana

    PubMed Central

    Bouché, Frédéric; Lobet, Guillaume; Tocquin, Pierre; Périlleux, Claire

    2016-01-01

    Flowering is a hot topic in Plant Biology and important progress has been made in Arabidopsis thaliana toward unraveling the genetic networks involved. The increasing complexity and the explosion of literature however require development of new tools for information management and update. We therefore created an evolutive and interactive database of flowering time genes, named FLOR-ID (Flowering-Interactive Database), which is freely accessible at http://www.flor-id.org. The hand-curated database contains information on 306 genes and links to 1595 publications gathering the work of >4500 authors. Gene/protein functions and interactions within the flowering pathways were inferred from the analysis of related publications, included in the database and translated into interactive manually drawn snapshots. PMID:26476447

  9. Evaluation of the chromogenic agar chromID C. difficile.

    PubMed

    Eckert, Catherine; Burghoffer, Béatrice; Lalande, Valérie; Barbut, Frederic

    2013-03-01

    Three selective media (chromID C. difficile agar, taurocholate cycloserine cefoxitin agar [TCCA; homemade], and CLO medium) were compared from 406 stool samples of patients suspected of having Clostridium difficile infection. The sensitivities of chromID C. difficile agar at 24 h and 48 h, CLO medium, and TCCA were 74.1%, 87%, 85.2%, and 70.4%, respectively.

  10. Id4 Marks Spermatogonial Stem Cells in the Mouse Testis.

    PubMed

    Sun, Feng; Xu, Qing; Zhao, Danfeng; Degui Chen, Charlie

    2015-01-01

    Mammalian spermatogenesis is a classic adult stems cell-dependent process, supported by the self-renewal and differentiation of spermatogonial stem cells (SSCs). However, the identification of SSCs and elucidation of their behaviors in undisturbed testis has long been a big challenge. Here, we generated a knock-in mouse model, Id4-2A-CreERT2-2A-tdTomato, which allowed us to mark Id4-expressing (Id4(+)) cells at different time points in situ and track their behaviors across distinct developmental stages during steady-state and regenerating spermatogenesis. We found that Id4(+) cells continue to produce spermatogonia, spermatocytes and sperm in mouse testis, showing they are capable of self-renewal and have differentiation potential. Consistent with these findings, ablation of Id4(+) cells in mice results in a loss of spermatogenesis. Furthermore, developmental fate mapping reveals that Id4(+) SSCs originate from neonate Id4(+) gonocytes. Therefore, our results indicate that Id4 marks spermatogonial stem cells in the mouse testis.

  11. ID Model Construction and Validation: A Multiple Intelligences Case

    ERIC Educational Resources Information Center

    Tracey, Monica W.; Richey, Rita C.

    2007-01-01

    This is a report of a developmental research study that aimed to construct and validate an instructional design (ID) model that incorporates the theory and practice of multiple intelligences (MI). The study consisted of three phases. In phase one, the theoretical foundations of multiple Intelligences and ID were examined to guide the development…

  12. 75 FR 12974 - Establishment of Class E Airspace; Hailey, ID

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-03-18

    ... E airspace at Hailey, ID, to accommodate aircraft using the Area Navigation (RNAV) Global Positioning System (GPS) Standard Instrument Approach Procedure (SIAP) at Friedman Memorial Airport. This will... Register a notice of proposed rulemaking to establish additional controlled airspace at Hailey, ID (74...

  13. An ID2-dependent mechanism for VHL inactivation in cancer.

    PubMed

    Lee, Sang Bae; Frattini, Veronique; Bansal, Mukesh; Castano, Angelica M; Sherman, Dan; Hutchinson, Keino; Bruce, Jeffrey N; Califano, Andrea; Liu, Guangchao; Cardozo, Timothy; Iavarone, Antonio; Lasorella, Anna

    2016-01-14

    Mechanisms that maintain cancer stem cells are crucial to tumour progression. The ID2 protein supports cancer hallmarks including the cancer stem cell state. HIFα transcription factors, most notably HIF2α (also known as EPAS1), are expressed in and required for maintenance of cancer stem cells (CSCs). However, the pathways that are engaged by ID2 or drive HIF2α accumulation in CSCs have remained unclear. Here we report that DYRK1A and DYRK1B kinases phosphorylate ID2 on threonine 27 (Thr27). Hypoxia downregulates this phosphorylation via inactivation of DYRK1A and DYRK1B. The activity of these kinases is stimulated in normoxia by the oxygen-sensing prolyl hydroxylase PHD1 (also known as EGLN2). ID2 binds to the VHL ubiquitin ligase complex, displaces VHL-associated Cullin 2, and impairs HIF2α ubiquitylation and degradation. Phosphorylation of Thr27 of ID2 by DYRK1 blocks ID2-VHL interaction and preserves HIF2α ubiquitylation. In glioblastoma, ID2 positively modulates HIF2α activity. Conversely, elevated expression of DYRK1 phosphorylates Thr27 of ID2, leading to HIF2α destabilization, loss of glioma stemness, inhibition of tumour growth, and a more favourable outcome for patients with glioblastoma. PMID:26735018

  14. A Contextual Model for Identity Management (IdM) Interfaces

    ERIC Educational Resources Information Center

    Fuller, Nathaniel J.

    2014-01-01

    The usability of Identity Management (IdM) systems is highly dependent upon design that simplifies the processes of identification, authentication, and authorization. Recent findings reveal two critical problems that degrade IdM usability: (1) unfeasible techniques for managing various digital identifiers, and (2) ambiguous security interfaces.…

  15. Id2 Mediates Oligodendrocyte Precursor Cell Maturation Arrest and Is Tumorigenic in a PDGF-Rich Microenvironment

    PubMed Central

    Havrda, Matthew C.; Paolella, Brenton R.; Ran, Cong; Jering, Karola S.; Wray, Christina M.; Sullivan, Jaclyn M.; Nailor, Audrey; Hitoshi, Yasuyuki; Israel, Mark A.

    2016-01-01

    Maturation defects occurring in adult tissue progenitor cells have the potential to contribute to tumor development; however, there is little experimental evidence implicating this cellular mechanism in the pathogenesis of solid tumors. Inhibitor of DNA-binding 2 (Id2) is a transcription factor known to regulate the proliferation and differentiation of primitive stem and progenitor cells. Id2 is derepressed in adult tissue neural stem cells (NSC) lacking the tumor suppressor Tp53 and modulates their proliferation. Constitutive expression of Id2 in differentiating NSCs resulted in maturation-resistant oligodendroglial precursor cells (OPC), a cell population implicated in the initiation of glioma. Mechanistically, Id2 overexpression was associated with inhibition of the Notch effector Hey1, a bHLH transcription factor that we here characterize as a direct transcriptional repressor of the oligodendroglial lineage determinant Olig2. Orthotopic inoculation of NSCs with enhanced Id2 expression into brains of mice engineered to express platelet-derived growth factor in the central nervous system resulted in glioma. These data implicate a mechanism of altered NSC differentiation in glioma development and characterize a novel mouse model that reflects key characteristics of the recently described proneural subtype of glioblastoma multiforme. Such findings support the emerging concept that the cellular and molecular characteristics of tumor cells are linked to the transformation of distinct subsets of adult tissue progenitors. PMID:24425046

  16. 32 CFR 161.8 - ID card life-cycle roles and responsibilities.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 32 National Defense 1 2014-07-01 2014-07-01 false ID card life-cycle roles and responsibilities... IDENTIFICATION (ID) CARDS FOR MEMBERS OF THE UNIFORMED SERVICES, THEIR DEPENDENTS, AND OTHER ELIGIBLE INDIVIDUALS DoD Identification (ID) Cards: ID Card Life-Cycle § 161.8 ID card life-cycle roles...

  17. Id-1 and Id-2 genes and products as therapeutic targets for treatment of breast cancer and other types of carcinoma

    DOEpatents

    Desprez, Pierre-Yves; Campisi, Judith

    2014-09-30

    A method for treatment and amelioration of breast, cervical, ovarian, endometrial, squamous cells, prostate cancer and melanoma in a patient comprising targeting Id-1 or Id-2 gene expression with a delivery vehicle comprising a product which modulates Id-1 or Id-2 expression.

  18. Biometric authentication for ID cards with hologram watermarks

    NASA Astrophysics Data System (ADS)

    Croce Ferri, Lucilla; Mayerhoefer, Astrid; Frank, Marcus; Vielhauer, Claus; Steinmetz, Ralf

    2002-04-01

    We present an analysis of a new technique for the authentication of ID cardholders, based on the integration of a biometrics-based authentication system with digital watermarks. The chosen biometric authentication method is the dynamic signature verification of the ID cardholder, while we use a specific integrity watermark technique we developed, called Hologram Watermark, to embed and retrieve the off-line data onto an ID card. We take advantage of the fact, that two static biometric features (images of the user's face and signature) are already integral part of ID cards for manual verification and extent the stored biometric information by embedding on-line handwriting features of the signature as holographic watermarks in the overall image information of an ID card. Manipulation of any of the image information can be detected and will further disallow biometric verification of the forger. The Hologram Watermark technique produces content-related data using computer-generated hologram coding techniques. These data are embedded with watermarking techniques into the personal data printed on an ID card. The content-related data in this specific application are the dynamic features of the cardholder's signature. The main goal of this paper is to analyze the suitability of dynamic signature verification in combination with the Hologram Watermark technique, to facilitate automated user authentication based on information transparently embedded in ID cards.

  19. 76 FR 62387 - Public User ID Badging

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-10-07

    ...-coded user number and an expiration date. Users may renew their cards by validating and updating the... the authority provided in 41 CFR 102-81, the USPTO issues security identification badges to members of... of identification with photograph. The security badges include a color photograph of the user...

  20. X-Linked Intellectual Disability: Unique Vulnerability of the Male Genome

    ERIC Educational Resources Information Center

    Stevenson, Roger E.; Schwartz, Charles E.

    2009-01-01

    X-linked intellectual disability (XLID) accounts for approximately 16% of males with intellectual disability (ID). This is, in part, related to the fact that males have a single X chromosome. Progress in the clinical and molecular characterization of XLID has outpaced progress in the delineation of ID due to genes on the other 22 chromosomes.…

  1. Australian Curriculum Linked Lessons

    ERIC Educational Resources Information Center

    Hurrell, Derek; O'Neil, Jennifer

    2011-01-01

    In providing a continued focus on tasks and activities that help to illustrate key ideas embedded in the new Australian Curriculum, this issue the authors focus, on Geometry in the Measurement and Geometry strand with strong links for an integrated focus on the Statistics and Probability strand. The small unit of work on the sorting and…

  2. Education Studies: Issues & Critical Perspectives

    ERIC Educational Resources Information Center

    Kassem, Derek; Mufti, Emmanuel; Robinson, John

    2006-01-01

    This major text for Education Studies students provides a critical account of key issues in education today. The text features: (1) A critical analysis of key issues in Education Studies to encourage students' thinking about education in the broadest terms; (2) Themed sections with introductions to link the issues discussed in each chapter; (3)…

  3. Scientists ID Key Fetal Cells Vulnerable to Zika

    MedlinePlus

    ... html Scientists ID Key Fetal Cells Vulnerable to Zika Lab study suggests possible mechanism for birth defects ... 29, 2016 (HealthDay News) -- The devastating mosquito-borne Zika virus can infect cells that play a role ...

  4. IDS Combined Solution improvements between ITRF2008 and ITRF2013

    NASA Astrophysics Data System (ADS)

    Moreaux, Guilhem; Lemoine, Frank; Willis, Pascal; Capdeville, Hugues; Otten, Michiel; Stepanek, Petr; Kuzin, Sergei; Ferrage, Pascale

    2015-04-01

    In the context of ITRF 2013, the IDS Combination Center delivered to IERS weekly combined SINEX files from early January 1993 to late August 2014. These SINEX files which contain DORIS stations positions/velocities and Earth orientation parameters from DORIS data are based on the latest series of all of the 6 IDS Analysis Centers. The primary objective of this study is to present latest results from the DORIS contribution to ITRF2013. Then, comparisons with IDS contribution to ITRF2008 will address benefits of including both South Atlantic Anomaly corrected data and new DORIS missions (e.g. Jason-1, Jason-2, Cryosat-2) in the IDS combination. These comparisons will also emphasize application of DORIS ground antennas phase laws in the data processing and improved modeling of DORIS ground beacon frequency variations.

  5. Security analysis for biometric data in ID documents

    NASA Astrophysics Data System (ADS)

    Schimke, Sascha; Kiltz, Stefan; Vielhauer, Claus; Kalker, Ton

    2005-03-01

    In this paper we analyze chances and challenges with respect to the security of using biometrics in ID documents. We identify goals for ID documents, set by national and international authorities, and discuss the degree of security, which is obtainable with the inclusion of biometric into documents like passports. Starting from classical techniques for manual authentication of ID card holders, we expand our view towards automatic methods based on biometrics. We do so by reviewing different human biometric attributes by modality, as well as by discussing possible techniques for storing and handling the particular biometric data on the document. Further, we explore possible vulnerabilities of potential biometric passport systems. Based on the findings of that discussion we will expand upon two exemplary approaches for including digital biometric data in the context of ID documents and present potential risks attack scenarios along with technical aspects such as capacity and robustness.

  6. 76 FR 33651 - Approval and Promulgation of Implementation Plans; ID

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-06-09

    ... From the Federal Register Online via the Government Publishing Office ENVIRONMENTAL PROTECTION AGENCY 40 CFR Part 52 Approval and Promulgation of Implementation Plans; ID AGENCY: Environmental... includes new and revised rules which provide the Idaho Department of Environmental Quality (IDEQ)...

  7. A Good IDS Response Protocol of MANET Containment Strategies

    NASA Astrophysics Data System (ADS)

    Cheng, Bo-Chao; Chen, Huan; Tseng, Ryh-Yuh

    Much recent research concentrates on designing an Intrusion Detection System (IDS) to detect the misbehaviors of the malicious node in MANET with ad-hoc and mobility natures. However, without rapid and appropriate IDS response mechanisms performing follow-up management services, even the best IDS cannot achieve the desired primary goal of the incident response. A competent containment strategy is needed to limit the extent of an attack in the Incident Response Life Cycle. Inspired by the T-cell mechanisms in the human immune system, we propose an efficient MANET IDS response protocol (T-SecAODV) that can rapidly and accurately disseminate alerts of the malicious node attacks to other nodes so as to modify their AODV routing tables to isolate the malicious nodes. Simulations are conducted by the network simulator (Qualnet), and the experiment results indicate that T-SecAODV is able to spread alerts steadily while greatly reduce faulty rumors under simultaneous multiple malicious node attacks.

  8. Role of yeast domination in Indian idli batter fermentation.

    PubMed

    Soni, S K; Sandhu, D K

    1991-07-01

    Adding Saccharomyces cerevisiae, along with natural bacterial flora of the ingredients, was the best method for standardizing idli termentation in terms of improved organoleptic characteristics, leavening and nutritional constituents. PMID:24425138

  9. Node ID based detection of Sybil attack in mobile wireless sensor network

    NASA Astrophysics Data System (ADS)

    Sharmila, S.; Umamaheswari, G.

    2013-10-01

    Security is the major issue in wireless sensor networks and many defence mechanisms have been developed to secure the network from these alarming attacks by detecting the malicious nodes which hinder the performance of the network. Sybil attack can make the network vulnerable. Sybil attack means a node which illegitimately claims multiple identities. This attack threatens wireless sensor network in routing, voting system, fair resource allocation, data aggregation and misbehaviour detection. Hence, the research is carried out to prevent the Sybil attack and improve the network performance. The node ID-based scheme is proposed, where the detection is based on node registration, consisting of two phases and the assignment of ID to the node is done dynamically. The ID's corresponding to the nodes registered is at the base station and the node active time is monitored, any abnormalities in the above phases confirm the presence of Sybil nodes in the network. The scheme is simulated using NS2. The energy consumed for this algorithm is 2.3 J. The proposed detection scheme is analysed based on the network's PDR and found that the throughput has improved, which prove that this scheme may be used in the environment where security is needed.

  10. Biometrics and ID Cards — Enablers for Personal Security

    NASA Astrophysics Data System (ADS)

    Reisen, Andreas

    The electronic ID card is a modernization and security project of the German Government. On the one hand, the multifunctional card is intended to boost security and the convenience of e-government and e-business applications. On the other hand, the new biometric ID card should allow citizens to use it as a travel document in the Schengen area and for specific destinations outside the European Union also in the future.

  11. Multicultural Issues.

    ERIC Educational Resources Information Center

    Reid, Charrles; Kampfe, Charlene

    This chapter examines issues related to working with diverse populations with addictions. A brief history of multiculturalism and multicultural counseling is presented. Issues particular to the treatment of people with addictions are examined, as well as prevention and assessment issues. Substance abuse issues among people in the gay male and…

  12. Accelerated fermentation of 'idli' batter using soy residue okara.

    PubMed

    Rekha, C R; Vijayalakshmi, G

    2011-06-01

    The aim of this work was to reduce the natural fermentation period of 'idli' from the conventional 14 h to 10 h by adding underutilized okara for the preparation of 'idli'. Black gram was partially substituted with soy residue okara in the ratio of (1:1). After 14 h of natural fermentation, the pH and total acidity of control 'idli' batter was 4.51 and 0.64% and that of okara fortified 'idli' batter was 4.53 and 0.43%, respectively. The amount of CO2 released by the control and okara fortified batter was 19.7% and 33.6% respectively. The viable count of yeast and mold, lactics and mesophilic bacteria in control & okara batter increased with time reaching 9.00 & 10.34, 8.66 & 7.69, and 8.65 & 9.47 log10 cfu/g, respectively at the end of 10 h of natural fermentation. Okara fortified 'idli' was soft and spongy compared to control 'idli' .

  13. BioID Identification of Lamin-Associated Proteins.

    PubMed

    Mehus, Aaron A; Anderson, Ruthellen H; Roux, Kyle J

    2016-01-01

    A- and B-type lamins support the nuclear envelope, contribute to heterochromatin organization, and regulate a myriad of nuclear processes. The mechanisms by which lamins function in different cell types and the mechanisms by which lamin mutations cause over a dozen human diseases (laminopathies) remain unclear. The identification of proteins associated with lamins is likely to provide fundamental insight into these mechanisms. BioID (proximity-dependent biotin identification) is a unique and powerful method for identifying protein-protein and proximity-based interactions in living cells. BioID utilizes a mutant biotin ligase from bacteria that is fused to a protein of interest (bait). When expressed in living cells and stimulated with excess biotin, this BioID-fusion protein promiscuously biotinylates directly interacting and vicinal endogenous proteins. Following biotin-affinity capture, the biotinylated proteins can be identified using mass spectrometry. BioID thus enables screening for physiologically relevant protein associations that occur over time in living cells. BioID is applicable to insoluble proteins such as lamins that are often refractory to study by other methods and can identify weak and/or transient interactions. We discuss the use of BioID to elucidate novel lamin-interacting proteins and its applications in a broad range of biological systems, and provide detailed protocols to guide new applications.

  14. Accelerated fermentation of 'idli' batter using soy residue okara.

    PubMed

    Rekha, C R; Vijayalakshmi, G

    2011-06-01

    The aim of this work was to reduce the natural fermentation period of 'idli' from the conventional 14 h to 10 h by adding underutilized okara for the preparation of 'idli'. Black gram was partially substituted with soy residue okara in the ratio of (1:1). After 14 h of natural fermentation, the pH and total acidity of control 'idli' batter was 4.51 and 0.64% and that of okara fortified 'idli' batter was 4.53 and 0.43%, respectively. The amount of CO2 released by the control and okara fortified batter was 19.7% and 33.6% respectively. The viable count of yeast and mold, lactics and mesophilic bacteria in control & okara batter increased with time reaching 9.00 & 10.34, 8.66 & 7.69, and 8.65 & 9.47 log10 cfu/g, respectively at the end of 10 h of natural fermentation. Okara fortified 'idli' was soft and spongy compared to control 'idli' . PMID:23572755

  15. Optimized use of a 50mum ID secondary column in comprehensive two-dimensional gas chromatography-mass spectrometry.

    PubMed

    Tranchida, Peter Quinto; Purcaro, Giorgia; Fanali, Chiara; Dugo, Paola; Dugo, Giovanni; Mondello, Luigi

    2010-06-18

    The objective of the present research is directed towards the optimized use of a 50microm ID secondary column, in a comprehensive two-dimensional gas chromatography-quadrupole mass spectrometry (GCxGC-qMS) system. The analytical aim was achieved by exploiting a split-flow GCxGC approach, and a rapid-scanning qMS instrument. The stationary phase combination consisted of an apolar (silphenylene polymer) 30mx0.25mm ID column, linked by means of a Y-union, to an MS-connected 1mx0.05mm ID polar one [poly(ethyleneglycol)], and to a 0.20mx0.05mm ID uncoated capillary segment; the latter was connected to a manually operated split-valve. It will be herein demonstrated that the split-flow GCxGC approach, successfully employed in previous H(2)-based, flame ionization detection experiments, provides equally satisfactory results using mass spectrometric detection and helium as carrier gas. An optimized split-flow GCxGC-qMS method was developed and exploited for the analysis of a perfume sample. The results attained were compared with those observed using the same analytical column combination, but with no flow-splitting. It was found that it is not convenient to employ a 50microm ID secondary column in a conventional GCxGC-MS instrument. On the contrary, the use a 50microm ID secondary column, in a split-flow, twin-oven system, provided a good performance. A recently developed comprehensive chromatography software was used for data processing.

  16. Empirical analysis of Internet telephone network: From user ID to phone

    NASA Astrophysics Data System (ADS)

    Xuan, Qi; Du, Fang; Wu, Tie-Jun

    2009-06-01

    In order to study the interaction between different communication networks, in this paper, personal computer (PC)-to-phone log data in the year of 2007 are collected from UUCALL database and described as an ID-to-phone bipartite network (ItPBN). The ItPBN contains one giant component (GC) and a large number of satellitic components (SCs), both of which are carefully analyzed. It is found that the ItPBN has power-law incoming/outgoing degree distributions as well as a power-law clustering function (by proposing a new definition of clustering coefficient) indicating a hierarchical and modular structure of the ItPBN. Furthermore, the fact that most of the weak links always surrounding those ID nodes of large degree in the GC suggests that weak links may be more important to keep the structure of the GC than those strong ones. More interestingly, it is also revealed that there is strong correlation between many statistical properties of different SCs and their size, these extra information may be very useful in modeling the ItPBN in the future.

  17. Using ATM over SATCOM links

    NASA Technical Reports Server (NTRS)

    Comparetto, Gary M.

    1995-01-01

    The Asynchronous Transfer Mode (ATM) protocol is studied from the standpoint of determining what limitations, if any, exist in using it over satellite links. It is concluded that, while there is nothing intrinsic about ATM that would generally preclude its use over satellite links, there are, however, several intrinsic characteristics of satellite links, as well as some satellite system configuration-specific issues, that must be taken into account.

  18. Community Links

    ERIC Educational Resources Information Center

    Nelson, Mary

    1975-01-01

    At Moraine Valley Community College (Illinois), a chain of events, programs, activities, and services has linked the college and community in such areas as fine arts, ethnic groups, public services, community action, community service, and community education. (Author/NHM)

  19. Link Analysis

    NASA Astrophysics Data System (ADS)

    Donoho, Steve

    Link analysis is a collection of techniques that operate on data that can be represented as nodes and links. This chapter surveys a variety of techniques including subgraph matching, finding cliques and K-plexes, maximizing spread of influence, visualization, finding hubs and authorities, and combining with traditional techniques (classification, clustering, etc). It also surveys applications including social network analysis, viral marketing, Internet search, fraud detection, and crime prevention.

  20. Threshold Levels of Gfi1 Maintain E2A Activity for B Cell Commitment via Repression of Id1

    PubMed Central

    Fraszczak, Jennifer; Helness, Anne; Chen, Riyan; Vadnais, Charles; Robert, François; Khandanpour, Cyrus; Möröy, Tarik

    2016-01-01

    A regulatory circuit that controls myeloid versus B lymphoid cell fate in hematopoietic progenitors has been proposed, in which a network of the transcription factors Egr1/2, Nab, Gfi1 and PU.1 forms the core element. Here we show that a direct link between Gfi1, the transcription factor E2A and its inhibitor Id1 is a critical element of this regulatory circuit. Our data suggest that a certain threshold of Gfi1 is required to gauge E2A activity by adjusting levels of Id1 in multipotent progenitors, which are the first bipotential myeloid/lymphoid-restricted progeny of hematopoietic stem cells. If Gfi1 levels are high, Id1 is repressed enabling E2A to activate a specific set of B lineage genes by binding to regulatory elements for example the IL7 receptor gene. If Gfi1 levels fall below a threshold, Id1 expression increases and renders E2A unable to function, which prevents hematopoietic progenitors from engaging along the B lymphoid lineage. PMID:27467586

  1. Novel ID-based anti-collision approach for RFID

    NASA Astrophysics Data System (ADS)

    Zhang, De-Gan; Li, Wen-Bin

    2016-09-01

    Novel correlation ID-based (CID) anti-collision approach for RFID under the banner of the Internet of Things (IOT) has been presented in this paper. The key insights are as follows: according to the deterministic algorithms which are based on the binary search tree, we propose a method to increase the association between tags so that tags can initiatively send their own ID under certain trigger conditions, at the same time, we present a multi-tree search method for querying. When the number of tags is small, by replacing the actual ID with the temporary ID, it can greatly reduce the number of times that the reader reads and writes to tag's ID. Active tags send data to the reader by the way of modulation binary pulses. When applying this method to the uncertain ALOHA algorithms, the reader can determine the locations of the empty slots according to the position of the binary pulse, so it can avoid the decrease in efficiency which is caused by reading empty slots when reading slots. Theory and experiment show that this method can greatly improve the recognition efficiency of the system when applied to either the search tree or the ALOHA anti-collision algorithms.

  2. Emotional Issues

    MedlinePlus

    ... Coping with Duchenne Depression & Anxiety Connecting With Him Social Isolation Daily Life Handling His Questions Related links The psychology of Duchenne Muscular Dystrophy (download) Our family-centered ...

  3. 78 FR 773 - Hartford Financial Services Group, Inc., Commercial/Actuarial/Information Delivery Services (IDS...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-01-04

    .../ Information Delivery Services (IDS)/Corporate & Financial Reporting Group, Hartford, CT; Notice of Affirmative... (IDS)/Corporate & Financial Reporting group, Hartford, Connecticut (The Hartford-IDS Group). The... basis for the negative determination. The Hartford-IDS Group is engaged in activities related to...

  4. Long range thermal weapon sights for the German future infantryman program IdZ

    NASA Astrophysics Data System (ADS)

    Breiter, Rainer; Ihle, Tobias; Mauk, Karl-Heinz; Münzberg, Mario; Rode, Werner

    2007-04-01

    In December 2004 AIM started the series production of the HuntIR long range thermal weapon sight. The sight is fielded in the Germany Future Infantryman (IdZ) basic system and since that time in continuous service in various out of area missions with German participation. For very long identification ranges >1500m cooled technology still outperforms uncooled sights, even with respect to smaller size and lower weight because the typical F/1 design of uncooled systems overcompensates cooler weight for focal length >175mm. The HuntIR sight is therefore based on a cooled MWIR detection module for long range battlefield surveillance and target engagement. The device specifically is a perfect match to state of the art small arms like 0.50 cal sniper rifles or crew served weapons like the 40mm high velocity grenade machine gun (GMG) which provide engagement ranges >1500m and need an adequate sight performance beyond that. A recent modification of HuntIR was done to provide a wider field of view for improved situation awareness in urban operations and specifically to allow the engagement of the 40mm GMG in ranges between 250-1200m. The qualification tests of the sight by the German infantry were successfully completed mid 2006. To match the demand of the follow-up program IdZ-ES additional components have to be integrated. Most important are a laser range finder (LRF), 3 axis digital magnetic compass (DMC) and a wireless data link. LRF and DMC together with a highly sophisticated fire control computer provide improved first round hit probability, the DMC additionally improves the fire control in any case of steep trajectories or for pronounced ballistic trajectories to avoid any need to precisely level the GMG. This new sight is done under the brand name RangIR. An important additional feature is the interface for air burst ammunition (ABM). The optical distance is measured by the LRF, the fire control computer accurately evaluates the trajectory under the given angle

  5. Fulminant atypical Cryptococcus neoformans pneumonia confirmed by PLEX-ID.

    PubMed

    Gariani, Karim; Rougemont, Mathieu; Renzi, Gesuele; Hibbs, Jonathan; Emonet, Stéphane; Schrenzel, Jacques

    2014-05-01

    Use of the PLEX-ID system can lead to a rapid molecular diagnosis in microbiology. To illustrate the clinical implications of this new diagnostic tool, we present the case of a 46-year-old patient admitted with severe respiratory failure and septic shock. Cryptococcal pneumonia was diagnosed by Fungi-Fluor™ staining of the bronchoalveolar lavage (BAL) and the patient tested positive for HIV. Unfortunately, he died 12h after admission despite intensive care support and treatment with broad-spectrum antibiotics, amphotericin B, and flucytosine. Retrospective use of the PLEX-ID on the BAL, bronchial aspirate, and blood yielded Cryptococcus neoformans in all fluids tested. Rapid molecular diagnosis with PLEX-ID, especially when performed on the blood of septic patients, may reduce the time to adequate treatment and limit the number of diagnostic procedures needed.

  6. Effect of ingredients on sensory profile of idli.

    PubMed

    Durgadevi, Manoharan; Shetty, Prathapkumar H

    2014-09-01

    Idli is a traditional fermented food and is consumed in India and Srilanka. The objective of the present study is to select the ingredients for optimum desirable product characteristics and to identify the optimum ratios of ingredients and fermentation time with respect to sensory attributes using Response Surface Methodology (RSM). The sensory attributes included were color, appearance, texture, taste and overall quality. Preliminary trials were conducted using five variants of rice and common black gram dhal before framing a model using Central Composite Rotatable Design (CCRD). From the study it was found that a desirable score of 0.7439 was obtained for sensory attributes of idli made with the ratio of 3: 1.475 for IR20 idli rice and ADT3 variety black gram (with husk removed after soaking) fermented for 10.2 h. Principal Component Analysis (PCA) helped to discriminate the samples and attributes within the data matrix, depending upon their inter relationships. PMID:25190832

  7. Strengthening the Reporting of Molecular Epidemiology for Infectious Diseases (STROME-ID): an extension of the STROBE statement.

    PubMed

    Field, Nigel; Cohen, Ted; Struelens, Marc J; Palm, Daniel; Cookson, Barry; Glynn, Judith R; Gallo, Valentina; Ramsay, Mary; Sonnenberg, Pam; Maccannell, Duncan; Charlett, Andre; Egger, Matthias; Green, Jonathan; Vineis, Paolo; Abubakar, Ibrahim

    2014-04-01

    Molecular data are now widely used in epidemiological studies to investigate the transmission, distribution, biology, and diversity of pathogens. Our objective was to establish recommendations to support good scientific reporting of molecular epidemiological studies to encourage authors to consider specific threats to valid inference. The statement Strengthening the Reporting of Molecular Epidemiology for Infectious Diseases (STROME-ID) builds upon the Strengthening the Reporting of Observational Studies in Epidemiology (STROBE) initiative. The STROME-ID statement was developed by a working group of epidemiologists, statisticians, bioinformaticians, virologists, and microbiologists with expertise in control of infection and communicable diseases. The statement focuses on issues relating to the reporting of epidemiological studies of infectious diseases using molecular data that were not addressed by STROBE. STROME-ID addresses terminology, measures of genetic diversity within pathogen populations, laboratory methods, sample collection, use of molecular markers, molecular clocks, timeframe, multiple-strain infections, non-independence of infectious-disease data, missing data, ascertainment bias, consistency between molecular and epidemiological data, and ethical considerations with respect to infectious-disease research. In total, 20 items were added to the 22 item STROBE checklist. When used, the STROME-ID recommendations should advance the quality and transparency of scientific reporting, with clear benefits for evidence reviews and health-policy decision making.

  8. Installation, commissioning and performance of IDs installed at ALBA

    NASA Astrophysics Data System (ADS)

    Campmany, J.; Marcos, J.; Massana, V.; Becheri, F.; Gigante, J. V.; Colldelram, C.; Ribó, Ll

    2013-03-01

    The new synchrotron light source ALBA is currently starting regular operation. Up to 6 beamlines are using light produced by Insertion Devices. There are up to four types of IDs: 2 Apple-II undulators (EU62 and EU71) operating at low energies, one conventional wiggler (MPW80) operating in the range of 2 - 20 keV, two in-vacuum undulators (IVU21) operating in the range 5 - 30 keV and a superconducting wiggler (SCW30) operating in the range of (up to) 40 keV. The main IDs characteristics, their influence on the beam dynamics and a first characterization of their light will be presented.

  9. The effect of IDS (immediate dentin sealing) on dentin bond strength under various thermocycling periods

    PubMed Central

    Leesungbok, Richard; Lee, Sang-Min; Park, Su-Jung; Lee, Suk-Won; Lee, Do Yun; Im, Byung-Jin

    2015-01-01

    PURPOSE The purpose of this study was to find out the effect of immediate dentin sealing (IDS) on bond strength of ceramic restoration under various thermocycling periods with DBA (dentin bonding agent system). MATERIALS AND METHODS Fifty freshly extracted human mandibular third molars were divided into 5 groups (1 control and 4 experimental groups) of 10 teeth. We removed enamel layer of sound teeth and embedded them which will proceed to be IDS, using All Bond II. A thermocycling was applied to experimental groups for 1, 2, 7, 14 days respectively and was not applied to control group. IPS Empress II for ceramic was acid-etched with ceramic etchant (9.5% HF) and silane was applied. Each ceramic disc was bonded to specimens with Duo-link, dual curable resin cement by means of light curing for 100 seconds. After the cementation procedures, shear bond strength measurement and SEM analysis of the fractured surface were done. The data were analyzed with a one-way ANOVA and Tukey multiple comparison test (α=.05). RESULTS There were no statistically significant differences between 4 experimental groups and control group, however the mean value started to decrease in group 7d, and group 14d showed the lowest mean bond strength in all groups. Also, group 7d and 14d showed distinct exposed dentin and collapsed hybrid layer was observed in SEM analysis. CONCLUSION In the present study, it can be concluded that ceramic restorations like a laminate veneer restoration should be bonded using resin cement within one week after IDS procedure. PMID:26140174

  10. Commentary: RCT of Optimal Dose Methylphenidate in Children and Adolescents with Severe ADHD and ID--A Reply to Arnold (2013)

    ERIC Educational Resources Information Center

    Simonoff, Emily; Taylor, Eric; Baird, Gillian; Bernard, Sarah

    2013-01-01

    The commentary by Arnold (2013) raises a number of interesting issues and speculations about the action of methylphenidate in children with intellectual disability (ID) and associated neurodevelopmental disorders, such as autism spectrum disorders. In our article (Simonoff et al., 2013), we were careful to stick closely to the statistical analysis…

  11. Phosphorylation Regulates Id2 Degradation and Mediates the Proliferation of Neural Precursor Cells

    PubMed Central

    Sullivan, Jaclyn M.; Havrda, Matthew C.; Kettenbach, Arminja N.; Paolella, Brenton R.; Zhang, Zhonghua; Gerber, Scott A.; Israel, Mark A.

    2016-01-01

    Inhibitor of DNA binding proteins (Id1-Id4) function to inhibit differentiation and promote proliferation of many different cell types. Among the Id family members, Id2 has been most extensively studied in the central nervous system (CNS). Id2 contributes to cultured neural precursor cell (NPC) proliferation as well as to the proliferation of CNS tumors such as glioblastoma that are likely to arise from NPC-like cells. We identified three phosphorylation sites near the N-terminus of Id2 in NPCs. To interrogate the importance of Id2 phosphorylation, Id2−/− NPCs were modified to express wild type (WT) Id2 or an Id2 mutant protein that could not be phosphorylated at the identified sites. We observed that NPCs expressing this mutant lacking phosphorylation near the N-terminus had higher steady-state levels of Id2 when compared to NPCs expressing WT Id2. This elevated level was the result of a longer half-life and reduced proteasome-mediated degradation. Moreover, NPCs expressing constitutively de-phosphorylated Id2 proliferated more rapidly than NPCs expressing WT Id2, a finding consistent with the well-characterized function of Id2 in driving proliferation. Observing that phosphorylation of Id2 modulates the degradation of this important cell-cycle regulator, we sought to identify a phosphatase that would stabilize Id2 enhancing its activity in NPCs and extended our analysis to include human glioblastoma-derived stem cells (GSCs). We found that expression of the phosphatase PP2A altered Id2 levels. Our findings suggest that inhibition of PP2A may be a novel strategy to regulate the proliferation of normal NPCs and malignant GSCs by decreasing Id2 levels. PMID:26756672

  12. 78 FR 65555 - Establishment of Class E Airspace; Salmon, ID

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-11-01

    ... proposed rulemaking (NPRM) to establish controlled airspace at Salmon, ID (78 FR 45478). Interested parties...'' under DOT Regulatory Policies and Procedures (44 FR 11034; February 26, 1979); and (3) does not warrant... FR 9565, 3 CFR, 1959-1963 Comp., p. 389. Sec. 71.1 0 2. The incorporation by reference in 14 CFR...

  13. 77 FR 55688 - Amendment of Class E Airspace; Boise, ID

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-09-11

    ... E airspace designated as an extension at Boise Air Terminal, Boise, ID (77 FR 38552). Interested... FR 6026). Interested parties were invited to participate in this rulemaking effort by submitting... Regulatory Policies and Procedures (44 FR 11034; February 26, 1979); and (3) does not warrant preparation...

  14. 76 FR 70865 - Modification of Class E Airspace; Driggs, ID

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-11-16

    ...), 40103, 40113, 40120; E. O. 10854, 24 FR 9565, 3 CFR, 1959-1963 Comp., p. 389. Sec. 71.1 0 2. The... proposed rulemaking to modify controlled airspace at Driggs, ID (76 FR 56356). Interested parties were... a ``significant rule'' under DOT Regulatory Policies and Procedures (44 FR 11034; February 26,...

  15. 75 FR 50777 - Minidoka Dam Spillway Replacement, Minidoka County, ID

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-08-17

    ... on December 11, 2009 (74 FR 65783) with a public comment period ending on February 5, 2010. The Final... Bureau of Reclamation Minidoka Dam Spillway Replacement, Minidoka County, ID AGENCY: Bureau of... proposed Minidoka Dam Spillway Replacement. The U.S. Fish and Wildlife Service (Department of Interior)...

  16. Student ID Cards: What You Should Know About Them

    ERIC Educational Resources Information Center

    Hoffman, Jonathan; McGuire, Agnes C.

    1973-01-01

    Discusses the usefulness of photo ID cards for school security purposes, library control, student activities, and bus transportation control. Examines ways in which card costs can be reduced and the pros and cons of producing the cards at the school or of letting the work out. Problems involving card abuse and student rights are also considered.…

  17. idRHa+ProMod - Rail Hardening Control System

    NASA Astrophysics Data System (ADS)

    Ferro, L.

    2016-03-01

    idRHa+ProMod is the process control system developed by Primetals Technologies to foresee the thermo-mechanical evolution and micro-structural composition of rail steels subjected to slack quenching into idRHa+ Rail Hardening equipments in a simulation environment. This tool can be used both off-line or in-line, giving the user the chance to test and study the best cooling strategies or letting the automatic control system free to adjust the proper cooling recipe. Optimization criteria have been tailored in order to determine the best cooling conditions according to the metallurgical requirements imposed by the main rail standards and also taking into account the elastoplastic bending phenomena occurring during all stages of the head hardening process. The computational core of idRHa+ProMod is a thermal finite element procedure coupled with special algorithms developed to work out the main thermo-physical properties of steel, to predict the non-isothermal austenite decomposition into all the relevant phases and subsequently to evaluate the amount of latent heat of transformation released, the compound thermal expansion coefficient and the amount of plastic deformation in the material. Air mist and air blades boundary conditions have been carefully investigated by means of pilot plant tests aimed to study the jet impingement on rail surfaces and the cooling efficiency at all working conditions. Heat transfer coefficients have been further checked and adjusted directly on field during commissioning. idRHa+ is a trademark of Primetals Technologies Italy Srl

  18. Retention Revisited: R=E, Id+E & In, Iv.

    ERIC Educational Resources Information Center

    Seidman, Alan

    1996-01-01

    Recent research on college student attrition is examined for trends, and it is suggested that the common perspective on retention and attrition is too narrow; it should be viewed from three perspectives: within-course retention; program retention; and institutional retention rate. Recommended for retention (R) is early (E) identification (Id) plus…

  19. 75 FR 61610 - Modification of Class E Airspace; Arco, ID

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-10-06

    ... Federal Register a notice of proposed rulemaking to amend controlled airspace at Arco, ID (75 FR 41773... Policies and Procedures (44 FR 11034; February 26, 1979); and (3) does not warrant preparation of a... U.S.C. 106(g), 40103, 40113, 40120; E.O. 10854, 24 FR 9565, 3 CFR, 1959-1963 Comp., p. 389. Sec....

  20. 76 FR 22863 - Designation for the Lewiston, ID Area

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-04-25

    ... hours (7 CFR 1.27(c)). SUPPLEMENTARY INFORMATION: In the January 4, 2011, Federal Register (76 FR 317... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF AGRICULTURE Grain Inspection, Packers and Stockyards Administration Designation for the Lewiston, ID Area...

  1. Imagery Measures of Ego, Id, Superego, and Identity: Validity Studies.

    ERIC Educational Resources Information Center

    Cartwright, D.; DeBruin, J.

    1988-01-01

    Five validity studies of the id, ego, superego, and identity scales of the Study of Imagery are reported, using undergraduate students. Multistage Bonferroni procedures are used to evaluate the significance of results. The scales are related to each other and to toughmindedness, self-control, and behavioral conflict. (TJH)

  2. Blueprints for Complex Learning: The 4C/ID-Model.

    ERIC Educational Resources Information Center

    van Merrienboer, Jeroen J. G.; Clark, Richard E.; de Croock, Marcel B. M.

    2002-01-01

    Describes the four-component instructional design system (4C/ID-model) developed for the design of training programs for complex skills. Discusses the structure of training blueprints for complex learning and associated instructional methods, focusing on learning tasks, supportive information, just-in-time information, and part-task practice.…

  3. Evolution of the ID Process at Sprint: A Practitioner's Perspective.

    ERIC Educational Resources Information Center

    Beard, Michael W.

    1999-01-01

    Provides instructional developers' (ID) insights and observations based on the experiences of a training and development organization, Sprint's University of Excellence (UE), as it evolved from a small group of 20-25 individuals in 1990 to a large business unit comprising almost 400 individuals in 1999. Discussion includes course development…

  4. An Application of Decision Tree Based on ID3

    NASA Astrophysics Data System (ADS)

    Xiaohu, Wang; Lele, Wang; Nianfeng, Li

    This article deals with the application of classical decision tree ID3 of the data mining in a certain site data. It constitutes a decision tree based on information gain and thus produces some useful purchasing behavior rules. It also proves that the decision tree has a wide applicable future in the sale field on site.

  5. Gender Issues.

    ERIC Educational Resources Information Center

    Ilfeld, Ellen M., Ed.; Hanssen, Elizabeth, Ed.

    1997-01-01

    This issue of "Coordinators' Notebook" focuses on gender issues in early childhood. The first article, "Both Halves of the Sky: Gender Socialization in the Early Years," focuses on the arguments that have led to an international call for increased participation of girls in education, an introduction to studies which map young children's…

  6. Issues Curriculum.

    ERIC Educational Resources Information Center

    Sando, Joe S.

    A program for teaching techniques of critical thinking on issues concerning American Indians was developed for students at Albuquerque Indian School. It was designed to include not only the students but also their families with learning activities that required consultation in search of answers or understanding. The first issue presented sought to…

  7. Identification and expression profile of Id1 in bighead carp in response to microcystin-LR.

    PubMed

    Li, Huiying; Cai, Yan; Xie, Ping; Chen, Jun; Hao, Le; Li, Guangyu; Xiong, Qian

    2012-09-01

    Microcystin-LR (MCLR) is a widespread cyanotoxin produced in algal blooms, and has potent hepatotoxicity and tumor-promoting activity. We cloned the full-length cDNA of Id1 in bighead carp. The full-length Id1 cDNA was 954bp and contained a 387bp ORF. Bighead carp Id1 shared high identity with zebrafish Id1 amino acid sequence, and phylogenetic analysis showed that teleost Id1 evolved closely. Bighead carp Id1 constitutively expressed in all tested tissues in normal. When tested at two different time points post exposure and at 3 different MCLR doses, Id1 expression increased in a time-dependent pattern, and Id1 expression in brain was very sensitive to MCLR exposure. The present study will help us to understand more about the evolution of Id1 molecule and its role in the MCLR induced cell differentiation and cancer promoting in bighead carp. PMID:22683524

  8. Comparison of Perinatal Risk Factors Associated with Autism Spectrum Disorder (ASD), Intellectual Disability (ID), and Co-occurring ASD and ID.

    PubMed

    Schieve, Laura A; Clayton, Heather B; Durkin, Maureen S; Wingate, Martha S; Drews-Botsch, Carolyn

    2015-08-01

    While studies report associations between perinatal outcomes and both autism spectrum disorder (ASD) and intellectual disability (ID), there has been little study of ASD with versus without co-occurring ID. We compared perinatal risk factors among 7547 children in the 2006-2010 Autism and Developmental Disability Monitoring Network classified as having ASD + ID, ASD only, and ID only. Children in all three groups had higher rates of preterm birth (PTB), low birth weight, small-for-gestational-age, and low Apgar score than expected based on the US birth cohort adjusted for key socio-demographic factors. Associations with most factors, especially PTB, were stronger for children with ID only than children with ASD + ID or ASD only. Associations were similar for children with ASD + ID and ASD only.

  9. Inhibitor of differentiation 4 (ID4) acts as an inhibitor of ID-1, -2 and -3 and promotes basic helix loop helix (bHLH) E47 DNA binding and transcriptional activity.

    PubMed

    Sharma, Pankaj; Chinaranagari, Swathi; Chaudhary, Jaideep

    2015-05-01

    The four known ID proteins (ID1-4, Inhibitor of Differentiation) share a homologous helix loop helix (HLH) domain and act as dominant negative regulators of basic-HLH transcription factors. ID proteins also interact with many non-bHLH proteins in complex networks. The expression of ID proteins is increasingly observed in many cancers. Whereas ID-1, ID-2 and ID-3, are generally considered as tumor promoters, ID4 on the contrary has emerged as a tumor suppressor. In this study we demonstrate that ID4 heterodimerizes with ID-1, -2 and -3 and promote bHLH DNA binding, essentially acting as an inhibitor of inhibitors of differentiation proteins. Interaction of ID4 was observed with ID1, ID2 and ID3 that was dependent on intact HLH domain of ID4. Interaction with bHLH protein E47 required almost 3 fold higher concentration of ID4 as compared to ID1. Furthermore, inhibition of E47 DNA binding by ID1 was restored by ID4 in an EMSA binding assay. ID4 and ID1 were also colocalized in prostate cancer cell line LNCaP. The alpha helix forming alanine stretch N-terminal, unique to HLH ID4 domain was required for optimum interaction. Ectopic expression of ID4 in DU145 prostate cancer line promoted E47 dependent expression of CDKNI p21. Thus counteracting the biological activities of ID-1, -2 and -3 by forming inactive heterodimers appears to be a novel mechanism of action of ID4. These results could have far reaching consequences in developing strategies to target ID proteins for cancer therapy and understanding biologically relevant ID-interactions.

  10. Inhibitor of Differentiation 4 (ID4) Acts as an Inhibitor of ID-1, -2 and -3 and Promotes basic Helix Loop Helix (bHLH) E47 DNA Binding and Transcriptional Activity

    PubMed Central

    Sharma, Pankaj; Chinaranagari, Swathi; Chaudhary, Jaideep

    2015-01-01

    The four known ID proteins (ID1-4, Inhibitor of Differentiation) share a homologous helix loop helix (HLH) domain and act as dominant negative regulators of basic-HLH transcription factors. ID proteins also interact with many non-bHLH proteins in complex networks. The expression of ID proteins is increasingly observed in many cancers. Whereas ID-1, ID-2 and ID-3, are generally considered as tumor promoters, ID4 on the contrary has emerged as a tumor suppressor. In this study we demonstrate that ID4 heterodimerizes with ID-1, -2 and -3 and promote bHLH DNA binding, essentially acting as an inhibitor of inhibitors of differentiation proteins. Interaction of ID4 was observed with ID1, ID2 and ID3 that was dependent on intact HLH domain of ID4. Interaction with bHLH protein E47 required almost 3 fold higher concentration of ID4 as compared to ID1. Furthermore, inhibition of E47 DNA binding by ID1 was restored by ID4 in an EMSA binding assay. ID4 and ID1 were also colocalized in prostate cancer cell line LNCaP. The alpha helix forming alanine stretch N-terminal, unique to HLH ID4 domain was required for optimum interaction. Ectopic expression of ID4 in DU145 prostate cancer line promoted E47 dependent expression of CDKNI p21. Thus counteracting the biological activities of ID-1, -2 and -3 by forming inactive heterodimers appears to be a novel mechanism of action of ID4. These results could have far reaching consequences in developing strategies to target ID proteins for cancer therapy and understanding biologically relevant ID- interactions. PMID:25778840

  11. Evaluation of red blood cell and platelet antigen genotyping platforms (ID CORE XT/ID HPA XT) in routine clinical practice

    PubMed Central

    Finning, Kirstin; Bhandari, Radhika; Sellers, Fiona; Revelli, Nicoletta; Villa, Maria Antonietta; Muñiz-Díaz, Eduardo; Nogués, Núria

    2016-01-01

    Background High-throughput genotyping platforms enable simultaneous analysis of multiple polymorphisms for blood group typing. BLOODchip® ID is a genotyping platform based on Luminex® xMAP technology for simultaneous determination of 37 red blood cell (RBC) antigens (ID CORE XT) and 18 human platelet antigens (HPA) (ID HPA XT) using the BIDS XT software. Materials and methods In this international multicentre study, the performance of ID CORE XT and ID HPA XT, using the centres’ current genotyping methods as the reference for comparison, and the usability and practicality of these systems, were evaluated under working laboratory conditions. DNA was extracted from whole blood in EDTA with Qiagen methodologies. Ninety-six previously phenotyped/genotyped samples were processed per assay: 87 testing samples plus five positive controls and four negative controls. Results Results were available for 519 samples: 258 with ID CORE XT and 261 with ID HPA XT. There were three “no calls” that were either caused by human error or resolved after repeating the test. Agreement between the tests and reference methods was 99.94% for ID CORE XT (9,540/9,546 antigens determined) and 100% for ID HPA XT (all 4,698 alleles determined). There were six discrepancies in antigen results in five RBC samples, four of which (in VS, N, S and Doa) could not be investigated due to lack of sufficient sample to perform additional tests and two of which (in S and C) were resolved in favour of ID CORE XT (100% accuracy). The total hands-on time was 28–41 minutes for a batch of 16 samples. Compared with the reference platforms, ID CORE XT and ID HPA XT were considered simpler to use and had shorter processing times. Discussion ID CORE XT and ID HPA XT genotyping platforms for RBC and platelet systems were accurate and user-friendly in working laboratory settings. PMID:26674823

  12. Subgroups associated with lower physical fitness in older adults with ID: results of the HA-ID study.

    PubMed

    Hilgenkamp, Thessa I M; van Wijck, Ruud; Evenhuis, Heleen M

    2014-02-01

    Although physical fitness is generally very low in older adults with intellectual disabilities (ID), levels may differ across subgroups. It is important to identify which subgroups need to be targeted specifically in physical activity and fitness interventions and reference values. Physical fitness was measured with box-and-block-test, response-time-test, Berg-balance-scale, walking speed, grip strength, 30s-chair-stand, 10 m incremental-shuttle-walking test and the extended modified-back-saver-sit-and-reach-test in a large sample of older adults with ID (n=1050), and subgroups associated with lower physical fitness levels were identified applying multivariate linear regression analyses. Both fixed personal characteristics such as being older, being female, having more severe ID and having Down syndrome and modifiable or preventable factors such as physical activity levels, mobility impairments and a need of more intensive care, are independently associated with lower levels of multiple physical fitness components. This first study identifies subgroups of older adults with ID which require adapted reference values, and subgroups that need to be specifically targeted in fitness promotion programs.

  13. Family Issues

    MedlinePlus

    ... not mean that everyone gets along all the time. Conflicts are a part of family life. Many things can lead to conflict, such as illness, disability, addiction, job loss, school problems, and marital issues. Listening to ...

  14. A Hunter Patient with a Severe Phenotype Reveals Two Large Deletions and Two Duplications Extending 1.2 Mb Distally to IDS Locus.

    PubMed

    Zanetti, Alessandra; Tomanin, Rosella; Rampazzo, Angelica; Rigon, Chiara; Gasparotto, Nicoletta; Cassina, Matteo; Clementi, Maurizio; Scarpa, Maurizio

    2014-01-01

    Mucopolysaccharidosis type II (Hunter syndrome, MPS II) is an X-linked lysosomal storage disorder caused by the deficit of iduronate 2-sulfatase (IDS), an enzyme involved in the glycosaminoglycans (GAGs) degradation. We here report the case of a 9-year-old boy who was diagnosed with an extremely severe form of MPS II at 10 months of age. Sequencing of the IDS gene revealed the deletion of exons 1-7, extending distally and removing the entire pseudogene IDSP1. The difficulty to define the boundaries of the deletion and the particular severity of the patient phenotype suggested to verify the presence of pathological copy number variations (CNVs) in the genome, by the array CGH (aCGH) technology. The examination revealed the presence of two deletions alternate with two duplications, overall affecting a region of about 1.2 Mb distally to IDS gene. This is the first complex rearrangement involving IDS and extending to a large region located distally to it described in a severe Hunter patient, as evidenced by the CNVs databases interrogated. The analysis of the genes involved in the rearrangement and of the disorders correlated with them did not help to clarify the phenotype observed in our patient, except for the deletion of the IDS gene, which explains per se the Hunter phenotype. However, this cannot exclude a potential "contiguous gene syndrome" as well as the future rising of additional pathological symptoms associated with the other extra genes involved in the identified rearrangement.

  15. Access Control for Mobile Assessment Systems Using ID.

    PubMed

    Nakayama, Masaharu; Ishii, Tadashi; Morino, Kazuma

    2015-01-01

    The assessment of shelters during disaster is critical to ensure the health of evacuees and prevent pandemic. In the Ishinomaki area, one of the areas most damaged by the Great East Japan Earthquake, the highly organized assessment helped to successfully manage a total of 328 shelters with a total of 46,480 evacuees. The input and analysis of vast amounts of data was tedious work for staff members. However, a web-based assessment system that utilized mobile devices was thought to decrease workload and standardize the evaluation form. The necessary access of information should be controlled in order to maintain individuals' privacy. We successfully developed an access control system using IDs. By utilizing a unique numerical ID, users can access the input form or assessment table. This avoids unnecessary queries to the server, resulting in a quick response and easy availability, even with poor internet connection. PMID:26262204

  16. Status and evolution of the ESRF beamline ID19

    SciTech Connect

    Weitkamp, Timm; Tafforeau, Paul; Boller, Elodie; Cloetens, Peter; Valade, Jean-Paul; Bernard, Pascal; Baruchel, Jose; Peyrin, Francoise; Helfen, Lukas

    2010-04-06

    The ESRF synchrotron beamline ID19, dedicated to full-field parallel-beam imaging techniques such as phase-contrast and absorption microtomography and X-ray topography, is one of the most versatile instruments of its kind. This paper presents key characteristics of ID19 in its present form, names examples for research and development performed on the beamline, and outlines the plans for an upgrade on the beamline in coming years, to adapt to the growing needs of the user community. The technical goals envisioned include an increase in available beam size and maximum photon energy, and a substantial increase in flux density for applications using beams of small and intermediate size.

  17. Status and evolution of the ESRF beamline ID19

    NASA Astrophysics Data System (ADS)

    Weitkamp, Timm; Tafforeau, Paul; Boller, Elodie; Cloetens, Peter; Valade, Jean-Paul; Bernard, Pascal; Peyrin, Françoise; Ludwig, Wolfgang; Helfen, Lukas; Baruchel, José

    2010-04-01

    The ESRF synchrotron beamline ID19, dedicated to full-field parallel-beam imaging techniques such as phase-contrast and absorption microtomography and X-ray topography, is one of the most versatile instruments of its kind. This paper presents key characteristics of ID19 in its present form, names examples for research and development performed on the beamline, and outlines the plans for an upgrade on the beamline in coming years, to adapt to the growing needs of the user community. The technical goals envisioned include an increase in available beam size and maximum photon energy, and a substantial increase in flux density for applications using beams of small and intermediate size.

  18. PROCESS WATER BUILDING, TRA605. SUMP TANK PUMP. COMPARE WITH ID33G247. ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    PROCESS WATER BUILDING, TRA-605. SUMP TANK PUMP. COMPARE WITH ID-33-G-247. INL NEGATIVE NO. 4378. Unknown Photographer, 3/5/1952 - Idaho National Engineering Laboratory, Test Reactor Area, Materials & Engineering Test Reactors, Scoville, Butte County, ID

  19. ETR BASEMENT, TRA642, INTERIOR. BASEMENT. CUBICLE INTERIOR (SEE PHOTOS ID33G101 ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    ETR BASEMENT, TRA-642, INTERIOR. BASEMENT. CUBICLE INTERIOR (SEE PHOTOS ID-33-G-101 AND ID-33-G-102) WITH TANK AND SODIUM-RELATED APPARATUS. CAMERA STANDS BEFORE ROLL-UP DOOR SHOWN IN PHOTO ID-33-G-101. INL NEGATIVE NO. HD24-3-3. Mike Crane, Photographer, 11/2000 - Idaho National Engineering Laboratory, Test Reactor Area, Materials & Engineering Test Reactors, Scoville, Butte County, ID

  20. Suppression of Chondrogenesis by Id Helix-Loop-Helix Proteins in Murine Embryonic Orofacial Tissue

    PubMed Central

    Mukhopadhyay, Partha; Rezzoug, Francine; Webb, Cynthia L.; Pisano, M. Michele; Greene, Robert M.

    2009-01-01

    Inhibitors of differentiation (Id) proteins are helix-loop-helix (HLH) transcription factors lacking a DNA binding domain. Id proteins modulate cell proliferation, apoptosis, and differentiation in embryonic/fetal tissue. Perturbation of any of these processes in cells of the developing orofacial region results in orofacial anomalies. Chondrogenesis, a process integral to normal orofacial ontogenesis, is known to be modulated, in part, by Id proteins. In the present study, the mRNA and protein expression patterns of Id1, Id2, Id3 and Id4 were examined in developing murine orofacial tissue in vivo, as well as in murine embryonic maxillary mesenchymal cells in vitro. The functional role of Ids during chondrogenesis was also explored in vitro. Results reveal that cells derived from developing murine orofacial tissue: (1) express Id1, Id2, Id3 and Id4 mRNAs and proteins on each of gestational days 12-14, (2) express all four Id proteins in a developmentally regulated manner, (3) undergo chondrogenesis and express genes encoding various chondrogenic marker proteins (e.g. Runx2, Type X collagen, Sox9) when cultured under micromass conditions, and (4) can have their chondrogenic potential regulated via alteration of Id protein function through overexpression of a basic HLH factor. In summary, results from the current report reveal for the first time, the expression of all four Id proteins in cells derived from developing murine orofacial tissue, and demonstrate a functional role for the Ids in regulating the ability of these cells to undergo chondrogenesis. PMID:19349107

  1. Mecp2 regulates neural cell differentiation by suppressing the Id1 to Her2 axis in zebrafish.

    PubMed

    Gao, Hai; Bu, Ye; Wu, Qing; Wang, Xu; Chang, Nannan; Lei, Lei; Chen, Shilin; Liu, Dong; Zhu, Xiaojun; Hu, Keping; Xiong, Jing-Wei

    2015-06-15

    Rett syndrome (RTT) is a progressive neurological disorder caused by mutations in the X-linked protein methyl-CpG-binding protein 2 (MeCP2). The endogenous function of MeCP2 during neural differentiation is still unclear. Here, we report that mecp2 is required for brain development in zebrafish. Mecp2 was broadly expressed initially in embryos and enriched later in the brain. Either morpholino knockdown or genetic depletion of mecp2 inhibited neuronal differentiation, whereas its overexpression promoted neuronal differentiation, suggesting an essential role of mecp2 in directing neural precursors into differentiated neurons. Mechanistically, her2 (the zebrafish ortholog of mammalian Hes5) was upregulated in mecp2 morphants in an Id1-dependent manner. Moreover, knockdown of either her2 or id1 fully rescued neuronal differentiation in mecp2 morphants. These results suggest that Mecp2 plays an important role in neural cell development by suppressing the Id1-Her2 axis, and provide new evidence that embryonic neural defects contribute to the later motor and cognitive dysfunctions in RTT.

  2. Use of Persistent Identifiers to link Heterogeneous Data Systems in the Integrated Earth Data Applications (IEDA) Facility

    NASA Astrophysics Data System (ADS)

    Hsu, L.; Lehnert, K. A.; Carbotte, S. M.; Arko, R. A.; Ferrini, V.; O'hara, S. H.; Walker, J. D.

    2012-12-01

    The Integrated Earth Data Applications (IEDA) facility maintains multiple data systems with a wide range of solid earth data types from the marine, terrestrial, and polar environments. Examples of the different data types include syntheses of ultra-high resolution seafloor bathymetry collected on large collaborative cruises and analytical geochemistry measurements collected by single investigators in small, unique projects. These different data types have historically been channeled into separate, discipline-specific databases with search and retrieval tailored for the specific data type. However, a current major goal is to integrate data from different systems to allow interdisciplinary data discovery and scientific analysis. To increase discovery and access across these heterogeneous systems, IEDA employs several unique IDs, including sample IDs (International Geo Sample Number, IGSN), person IDs (GeoPass ID), funding award IDs (NSF Award Number), cruise IDs (from the Marine Geoscience Data System Expedition Metadata Catalog), dataset IDs (DOIs), and publication IDs (DOIs). These IDs allow linking of a sample registry (System for Earth SAmple Registration), data libraries and repositories (e.g. Geochemical Research Library, Marine Geoscience Data System), integrated synthesis databases (e.g. EarthChem Portal, PetDB), and investigator services (IEDA Data Compliance Tool). The linked systems allow efficient discovery of related data across different levels of granularity. In addition, IEDA data systems maintain links with several external data systems, including digital journal publishers. Links have been established between the EarthChem Portal and ScienceDirect through publication DOIs, returning sample-level objects and geochemical analyses for a particular publication. Linking IEDA-hosted data to digital publications with IGSNs at the sample level and with IEDA-allocated dataset DOIs are under development. As an example, an individual investigator could sign up

  3. 17 CFR 274.402 - Form ID, uniform application for access codes to file on EDGAR.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... Forms for Electronic Filing § 274.402 Form ID, uniform application for access codes to file on EDGAR... its Password. Editorial Note: For Federal Register citations affecting Form ID, see the List of CFR... 17 Commodity and Securities Exchanges 3 2012-04-01 2012-04-01 false Form ID, uniform...

  4. 17 CFR 259.602 - Form ID, uniform application for access codes to file on EDGAR.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... OF 1935 Forms for Electronic Filing § 259.602 Form ID, uniform application for access codes to file on EDGAR. Form ID must be filed by registrants, third party filers, or their agents, to whom the... 17 Commodity and Securities Exchanges 3 2011-04-01 2011-04-01 false Form ID, uniform...

  5. 17 CFR 274.402 - Form ID, uniform application for access codes to file on EDGAR.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... Forms for Electronic Filing § 274.402 Form ID, uniform application for access codes to file on EDGAR... its Password. Editorial Note: For Federal Register citations affecting Form ID, see the List of CFR... 17 Commodity and Securities Exchanges 3 2011-04-01 2011-04-01 false Form ID, uniform...

  6. 17 CFR 259.602 - Form ID, uniform application for access codes to file on EDGAR.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... OF 1935 Forms for Electronic Filing § 259.602 Form ID, uniform application for access codes to file on EDGAR. Form ID must be filed by registrants, third party filers, or their agents, to whom the... 17 Commodity and Securities Exchanges 3 2010-04-01 2010-04-01 false Form ID, uniform...

  7. 17 CFR 274.402 - Form ID, uniform application for access codes to file on EDGAR.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... Forms for Electronic Filing § 274.402 Form ID, uniform application for access codes to file on EDGAR... its Password. Editorial Note: For Federal Register citations affecting Form ID, see the List of CFR... 17 Commodity and Securities Exchanges 3 2010-04-01 2010-04-01 false Form ID, uniform...

  8. 17 CFR 274.402 - Form ID, uniform application for access codes to file on EDGAR.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... Forms for Electronic Filing § 274.402 Form ID, uniform application for access codes to file on EDGAR... its Password. Editorial Note: For Federal Register citations affecting Form ID, see the List of CFR... 17 Commodity and Securities Exchanges 4 2014-04-01 2014-04-01 false Form ID, uniform...

  9. 17 CFR 274.402 - Form ID, uniform application for access codes to file on EDGAR.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... Forms for Electronic Filing § 274.402 Form ID, uniform application for access codes to file on EDGAR... its Password. Editorial Note: For Federal Register citations affecting Form ID, see the List of CFR... 17 Commodity and Securities Exchanges 3 2013-04-01 2013-04-01 false Form ID, uniform...

  10. 78 FR 8596 - Hartford Financial Services Group, Inc., Commercial/Actuarial/ Information Delivery Services (IDS...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-02-06

    ... was published in the Federal Register on January 4, 2013 (78 FR 773). The Hartford-IDS Group is.../ Information Delivery Services (IDS)/Corporate & Financial Reporting Group, Hartford, CT; Notice of Negative... Services Group, Inc., Commercial/Actuarial/Information Delivery Services (IDS)/Corporate &...

  11. 77 FR 37696 - Agency Information Collection Activities: Importer ID Input Record

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-06-22

    ... SECURITY U.S. Customs and Border Protection Agency Information Collection Activities: Importer ID Input... other Federal agencies to comment on an information collection requirement concerning the Importer ID... concerning the following information collection: Title: Importer ID Input Record. OMB Number: 1651-0064....

  12. 78 FR 67383 - Agency Information Collection Activities; Importer ID Input Record

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-11-12

    ... SECURITY U.S. Customs and Border Protection Agency Information Collection Activities; Importer ID Input... other Federal agencies to comment on an information collection requirement concerning the Importer ID... concerning the following information collection: Title: Importer ID Input Record. OMB Number: 1651-0064....

  13. A Role for Id2 in Regulating Photic Entrainment of the Mammalian Circadian System

    PubMed Central

    Duffield, Giles E.; Watson, Nathan P.; Mantani, Akio; Peirson, Stuart N.; Robles-Murguia, Maricela; Loros, Jennifer J.; Israel, Mark A.; Dunlap, Jay C.

    2009-01-01

    Summary Inhibitor of DNA binding genes (Id1–Id4) encode helix-loop-helix (HLH) transcriptional repressors associated with development and tumorigenesis [1, 2], but little is known concerning the function(s) of these genes in normal adult animals. Id2 was identified in DNA microarray screens for rhythmically expressed genes [3–5], and further analysis revealed a circadian pattern of expression of all four Id genes in multiple tissues including the suprachiasmatic nucleus. To explore an in vivo function, we generated and characterized deletion mutations of Id2 and of Id4. Id2−/− mice exhibit abnormally rapid entrainment and an increase in the magnitude of the phase shift of the pacemaker. A significant proportion of mice also exhibit disrupted rhythms when maintained under constant darkness. Conversely, Id4−/− mice did not exhibit a noticeable circadian phenotype. In vitro studies using an mPer1 and an AVP promoter reporter revealed the potential for ID1, ID2, and ID3 proteins to interact with the canonical basic HLH clock proteins BMAL1 and CLOCK. These data suggest that the Id genes may be important for entrainment and operation of the mammalian circadian system, potentially acting through BMAL1 and CLOCK targets. PMID:19217292

  14. Picture This: How to Establish an Effective School ID Card Program

    ERIC Educational Resources Information Center

    Finkelstein, David

    2013-01-01

    Most school districts do not have an ID card policy that everyone knows and follows, yet. many school districts are implementing ID card programs to address concerns about safety, efficiency, and convenience. A well-thought-out ID card program leads to greater security and smoother operations throughout the school and should thus be a priority.…

  15. Bond Issues.

    ERIC Educational Resources Information Center

    Pollack, Rachel H.

    2000-01-01

    Notes trends toward increased borrowing by colleges and universities and offers guidelines for institutions that are considering issuing bonds to raise money for capital projects. Discussion covers advantages of using bond financing, how use of bonds impacts on traditional fund raising, other cautions and concerns, and some troubling aspects of…

  16. Unaddressed Issues

    ERIC Educational Resources Information Center

    Rochester, J. Martin

    2005-01-01

    Walter Parker's January article, "Teaching Against Idiocy," raises important and fascinating issues relating to the proper role and function of the K-12 social studies classroom. Although J. Martin Rochester, the author of this article, agrees with his basic premise that schools obviously have an obligation to help promote citizenship education,…

  17. Faculty Issues.

    ERIC Educational Resources Information Center

    Finkelstein, Martin

    Patterns that emerged from reviewing syllabi for courses on faculty issues are discussed, and four sample syllabi are presented. Few doctoral programs in higher education administration were identified that devote an entire course to the subject of American college and university faculty. For four courses that did devote an entire course to the…

  18. Newspaper Issue.

    ERIC Educational Resources Information Center

    Bourke, Thomas A.; And Others

    1986-01-01

    This special issue includes "The Microfilming of Newspapers: An Overview" (Thomas Bourke); "United States Newspaper Program: Progress and Propsects" (Larry Sullivan); "The Preservation of Canadian Newspapers" (Mary Jane Starr); "Current Filming of the New York Times at UMI" (Kenneth Tillman); and "The Cooperative Africana Microform Project" (Ray…

  19. Id2 specifically alters regulation of the cell cycle by tumor suppressor proteins.

    PubMed Central

    Lasorella, A; Iavarone, A; Israel, M A

    1996-01-01

    Cells which are highly proliferative typically lack expression of differentiated, lineage-specific characteristics. Id2, a member of the helix-loop-helix (HLH) protein family known to inhibit cell differentiation, binds to the retinoblastoma protein (pRb) and abolishes its growth-suppressing activity. We found that Id2 but not Id1 or Id3 was able to bind in vitro not only pRb but also the related proteins p107 and p130. Also, an association between Id2 and p107 or p130 was observed in vivo in transiently transfected Saos-2 cells. In agreement with these results, expression of Id1 or Id3 did not affect the block of cell cycle progression mediated by pRb. Conversely, expression of Id2 specifically reversed the cell cycle arrest induced by each of the three members of the pRb family. Furthermore, the growth-suppressive activities of cyclin-dependent kinase inhibitors p16 and p21 were efficiently antagonized by high levels of Id2 but not by Id1 Id3. Consistent with the role of p16 as a selective inhibitor of pRb and pRb-related protein kinase activity, p16-imposed cell cycle arrest was completely abolished by Id2. Only a partial reversal of p21-induced growth suppression was observed, which correlated with the presence of a functional pRb. We also documented decreased levels of cyclin D1 protein and mRNA and the loss of cyclin D1-cdk4 complexes in cells constitutively expressing Id2. These data provide evidence for important Id2-mediated alterations in cell cycle components normally involved in the regulatory events of cell cycle progression, and they highlight a specific role for Id2 as an antagonist of multiple tumor suppressor proteins. PMID:8649364

  20. Measuring Link-Resolver Success: Comparing 360 Link with a Local Implementation of WebBridge

    ERIC Educational Resources Information Center

    Herrera, Gail

    2011-01-01

    This study reviewed link resolver success comparing 360 Link and a local implementation of WebBridge. Two methods were used: (1) comparing article-level access and (2) examining technical issues for 384 randomly sampled OpenURLs. Google Analytics was used to collect user-generated OpenURLs. For both methods, 360 Link out-performed the local…

  1. Structural organisation and chromosomal mapping of the human Id-3 gene.

    PubMed

    Deed, R W; Hirose, T; Mitchell, E L; Santibanez-Koref, M F; Norton, J D

    1994-12-30

    The helix-loop-helix (HLH) family of transcription factors plays a central role in the regulation of cell growth, differentiation and tumourigenesis. Members of the Id (inhibitor of DNA binding) class of these nuclear proteins are able to heterodimerise with and thereby antagonise the functions of other transcription factors of this family. We report here on the genomic organisation of the human Id3 (HLH 1R21/heir1) gene. Comparison with the two other mammalian Id genes, Id1 and Id2, reveals a highly conserved protein coding gene organisation consistent with evolution from a common, ancestral Id-like gene. In addition, by using a yeast artificial chromosome (YAC) clone of Id3, we have fine-scale mapped the gene to chromosome band 1p36.1 by fluorescence in situ hybridisation (FISH) and, using the same FISH technique, we have detected heterogeneity in tumour-associated 1p36 chromosome translocations.

  2. Impaired Thermogenesis and a Molecular Signature for Brown Adipose Tissue in Id2 Null Mice

    PubMed Central

    Zhou, Peng; Robles-Murguia, Maricela; Mathew, Deepa; Duffield, Giles E.

    2016-01-01

    Inhibitor of DNA binding 2 (ID2) is a helix-loop-helix transcriptional repressor rhythmically expressed in many adult tissues. Our previous studies have demonstrated that Id2 null mice have sex-specific elevated glucose uptake in brown adipose tissue (BAT). Here we further explored the role of Id2 in the regulation of core body temperature over the circadian cycle and the impact of Id2 deficiency on genes involved in insulin signaling and adipogenesis in BAT. We discovered a reduced core body temperature in Id2−/− mice. Moreover, in Id2−/− BAT, 30 genes including Irs1, PPARs, and PGC-1s were identified as differentially expressed in a sex-specific pattern. These data provide valuable insights into the impact of Id2 deficiency on energy homeostasis of mice in a sex-specific manner. PMID:27144179

  3. CRISP-ID: decoding CRISPR mediated indels by Sanger sequencing

    PubMed Central

    Dehairs, Jonas; Talebi, Ali; Cherifi, Yacine; Swinnen, Johannes V.

    2016-01-01

    The advent of next generation gene editing technologies has revolutionized the fields of genome engineering in allowing the generation of gene knockout models and functional gene analysis. However, the screening of resultant clones remains challenging due to the simultaneous presence of different indels. Here, we present CRISP-ID, a web application which uses a unique algorithm for genotyping up to three alleles from a single Sanger sequencing trace, providing a robust and readily accessible platform to directly identify indels and significantly speed up the characterization of clones. PMID:27363488

  4. Quadruplex Integrated DNA (QuID) Nanosensors for Monitoring Dopamine

    PubMed Central

    Morales, Jennifer M.; Skipwith, Christopher G.; Clark, Heather A.

    2015-01-01

    Dopamine is widely innervated throughout the brain and critical for many cognitive and motor functions. Imbalances or loss in dopamine transmission underlie various psychiatric disorders and degenerative diseases. Research involving cellular studies and disease states would benefit from a tool for measuring dopamine transmission. Here we show a Quadruplex Integrated DNA (QuID) nanosensor platform for selective and dynamic detection of dopamine. This nanosensor exploits DNA technology and enzyme recognition systems to optically image dopamine levels. The DNA quadruplex architecture is designed to be compatible in physically constrained environments (110 nm) with high flexibility, homogeneity, and a lower detection limit of 110 µM. PMID:26287196

  5. Multiple cryptic splice sites can be activated by IDS point mutations generating misspliced transcripts.

    PubMed

    Lualdi, Susanna; Pittis, Maria G; Regis, Stefano; Parini, Rossella; Allegri, Anna E; Furlan, Francesca; Bembi, Bruno; Filocamo, Mirella

    2006-08-01

    Mutations in the gene encoding the enzyme iduronate-2-sulfatase (IDS) were reported as the cause of the X-linked recessive lysosomal disease, mucopolysaccharidosis II (MPS II). Amongst the different mutations, it emerges that nearly 10% are nucleotide substitutions causing splicing mutations. We now report the molecular characterisation of three MPS II patients with multiple aberrant transcripts due to three different point mutations. The c.418+1G>C that occurred in the invariant splice-site motif, produced only aberrantly spliced transcripts. Whilst the mutations affecting variant motifs (c.419G>T) or coding regions (c.245C>T) led to aberrantly spliced transcripts in addition to correctly spliced transcripts with the respective predicted missense mutation, p.G140V or p.A82V. A combination of experimental tests and computational approaches were used to understand the molecular basis underlying the altered transcription patterns. In addition, by using real-time reverse transcriptase polymerase chain reaction, the reduction of mRNA amount in two patients observed was likely due to nonsense-mediated mRNA decay pathway. Overall, our results further emphasised the importance of cloning and sequencing independent transcripts to reveal less abundant, aberrant products, which often could not be detected by direct sequencing. Moreover, the different splicing patterns observed in the three patients as a consequence of point mutations show how sensitive the balance is between constitutive and cryptic splice sites in the IDS gene. The generation of such diverse transcripts, together with their level of expression, could contribute to the profound phenotypic variability reported in MPS II.

  6. Psychometric properties of the Chinese version of Inventory for Depressive Symptomatology (IDS): Preliminary findings.

    PubMed

    Wu, Daxing; Yin, Huifang; Xu, Shujing; Carmody, Thomas; Morris, David W

    2010-09-01

    The aim of this study was to provide psychometric properties of the validity and reliability of the 30-item Inventory of Depressive Symptomatology as both a self-report (IDS-SR(30)) and as a clinician rating scale (IDS-C(30)) in a group of Chinese depressed outpatients. The Chinese version of IDS-C(30), IDS-SR(30), HRSD(17), BDI(13), SDS and ATQ were completed for 64 Chinese outpatients with major depressive disorder. The Cronbach's alphas for IDS-C(30) and IDS-SR(30) were 0.799 and 0.805, respectively. The coefficients test-retest reliability for the IDS-C(30) was 0.824 and for the IDSR-SR(30) was 0.718. The Pearson correlation coefficients between the IDS-C(30) and IDS-SR(30), and the other symptom measures (HRSD(17), BDI(13), and SDS) were high (from 0.738 to 0.882). The intra-class correlation coefficient showed the strength of agreement between the IDS-C(30) and IDS-SR(30), and the other symptom measures was found to be adequate (0.634-0.780). The Kappa coefficients of the severity of IDS-C(30) to HRSD(17), IDS-SR(30) were 0.50 and 0.40, respectively. This pilot study finding showed reasonable validity and reliability for the Chinese version of IDS(30) with highly acceptable psychometric properties. The inventory is suitable for assessing the severity of Chinese depressed patients, but this needs to be confirmed using a larger sample. Further studies should be done to demonstrate sensitivity of the scales to change of depression.

  7. Microbe-ID: an open source toolbox for microbial genotyping and species identification

    PubMed Central

    Tabima, Javier F.; Everhart, Sydney E.; Larsen, Meredith M.; Weisberg, Alexandra J.; Kamvar, Zhian N.; Tancos, Matthew A.; Smart, Christine D.; Chang, Jeff H.

    2016-01-01

    Development of tools to identify species, genotypes, or novel strains of invasive organisms is critical for monitoring emergence and implementing rapid response measures. Molecular markers, although critical to identifying species or genotypes, require bioinformatic tools for analysis. However, user-friendly analytical tools for fast identification are not readily available. To address this need, we created a web-based set of applications called Microbe-ID that allow for customizing a toolbox for rapid species identification and strain genotyping using any genetic markers of choice. Two components of Microbe-ID, named Sequence-ID and Genotype-ID, implement species and genotype identification, respectively. Sequence-ID allows identification of species by using BLAST to query sequences for any locus of interest against a custom reference sequence database. Genotype-ID allows placement of an unknown multilocus marker in either a minimum spanning network or dendrogram with bootstrap support from a user-created reference database. Microbe-ID can be used for identification of any organism based on nucleotide sequences or any molecular marker type and several examples are provided. We created a public website for demonstration purposes called Microbe-ID (microbe-id.org) and provided a working implementation for the genus Phytophthora (phytophthora-id.org). In Phytophthora-ID, the Sequence-ID application allows identification based on ITS or cox spacer sequences. Genotype-ID groups individuals into clonal lineages based on simple sequence repeat (SSR) markers for the two invasive plant pathogen species P. infestans and P. ramorum. All code is open source and available on github and CRAN. Instructions for installation and use are provided at https://github.com/grunwaldlab/Microbe-ID. PMID:27602267

  8. Microbe-ID: an open source toolbox for microbial genotyping and species identification.

    PubMed

    Tabima, Javier F; Everhart, Sydney E; Larsen, Meredith M; Weisberg, Alexandra J; Kamvar, Zhian N; Tancos, Matthew A; Smart, Christine D; Chang, Jeff H; Grünwald, Niklaus J

    2016-01-01

    Development of tools to identify species, genotypes, or novel strains of invasive organisms is critical for monitoring emergence and implementing rapid response measures. Molecular markers, although critical to identifying species or genotypes, require bioinformatic tools for analysis. However, user-friendly analytical tools for fast identification are not readily available. To address this need, we created a web-based set of applications called Microbe-ID that allow for customizing a toolbox for rapid species identification and strain genotyping using any genetic markers of choice. Two components of Microbe-ID, named Sequence-ID and Genotype-ID, implement species and genotype identification, respectively. Sequence-ID allows identification of species by using BLAST to query sequences for any locus of interest against a custom reference sequence database. Genotype-ID allows placement of an unknown multilocus marker in either a minimum spanning network or dendrogram with bootstrap support from a user-created reference database. Microbe-ID can be used for identification of any organism based on nucleotide sequences or any molecular marker type and several examples are provided. We created a public website for demonstration purposes called Microbe-ID (microbe-id.org) and provided a working implementation for the genus Phytophthora (phytophthora-id.org). In Phytophthora-ID, the Sequence-ID application allows identification based on ITS or cox spacer sequences. Genotype-ID groups individuals into clonal lineages based on simple sequence repeat (SSR) markers for the two invasive plant pathogen species P. infestans and P. ramorum. All code is open source and available on github and CRAN. Instructions for installation and use are provided at https://github.com/grunwaldlab/Microbe-ID.

  9. Microbe-ID: an open source toolbox for microbial genotyping and species identification.

    PubMed

    Tabima, Javier F; Everhart, Sydney E; Larsen, Meredith M; Weisberg, Alexandra J; Kamvar, Zhian N; Tancos, Matthew A; Smart, Christine D; Chang, Jeff H; Grünwald, Niklaus J

    2016-01-01

    Development of tools to identify species, genotypes, or novel strains of invasive organisms is critical for monitoring emergence and implementing rapid response measures. Molecular markers, although critical to identifying species or genotypes, require bioinformatic tools for analysis. However, user-friendly analytical tools for fast identification are not readily available. To address this need, we created a web-based set of applications called Microbe-ID that allow for customizing a toolbox for rapid species identification and strain genotyping using any genetic markers of choice. Two components of Microbe-ID, named Sequence-ID and Genotype-ID, implement species and genotype identification, respectively. Sequence-ID allows identification of species by using BLAST to query sequences for any locus of interest against a custom reference sequence database. Genotype-ID allows placement of an unknown multilocus marker in either a minimum spanning network or dendrogram with bootstrap support from a user-created reference database. Microbe-ID can be used for identification of any organism based on nucleotide sequences or any molecular marker type and several examples are provided. We created a public website for demonstration purposes called Microbe-ID (microbe-id.org) and provided a working implementation for the genus Phytophthora (phytophthora-id.org). In Phytophthora-ID, the Sequence-ID application allows identification based on ITS or cox spacer sequences. Genotype-ID groups individuals into clonal lineages based on simple sequence repeat (SSR) markers for the two invasive plant pathogen species P. infestans and P. ramorum. All code is open source and available on github and CRAN. Instructions for installation and use are provided at https://github.com/grunwaldlab/Microbe-ID. PMID:27602267

  10. Microbe-ID: an open source toolbox for microbial genotyping and species identification

    PubMed Central

    Tabima, Javier F.; Everhart, Sydney E.; Larsen, Meredith M.; Weisberg, Alexandra J.; Kamvar, Zhian N.; Tancos, Matthew A.; Smart, Christine D.; Chang, Jeff H.

    2016-01-01

    Development of tools to identify species, genotypes, or novel strains of invasive organisms is critical for monitoring emergence and implementing rapid response measures. Molecular markers, although critical to identifying species or genotypes, require bioinformatic tools for analysis. However, user-friendly analytical tools for fast identification are not readily available. To address this need, we created a web-based set of applications called Microbe-ID that allow for customizing a toolbox for rapid species identification and strain genotyping using any genetic markers of choice. Two components of Microbe-ID, named Sequence-ID and Genotype-ID, implement species and genotype identification, respectively. Sequence-ID allows identification of species by using BLAST to query sequences for any locus of interest against a custom reference sequence database. Genotype-ID allows placement of an unknown multilocus marker in either a minimum spanning network or dendrogram with bootstrap support from a user-created reference database. Microbe-ID can be used for identification of any organism based on nucleotide sequences or any molecular marker type and several examples are provided. We created a public website for demonstration purposes called Microbe-ID (microbe-id.org) and provided a working implementation for the genus Phytophthora (phytophthora-id.org). In Phytophthora-ID, the Sequence-ID application allows identification based on ITS or cox spacer sequences. Genotype-ID groups individuals into clonal lineages based on simple sequence repeat (SSR) markers for the two invasive plant pathogen species P. infestans and P. ramorum. All code is open source and available on github and CRAN. Instructions for installation and use are provided at https://github.com/grunwaldlab/Microbe-ID.

  11. Molecular diagnosis of 65 families with mucopolysaccharidosis type II (Hunter syndrome) characterized by 16 novel mutations in the IDS gene: Genetic, pathological, and structural studies on iduronate-2-sulfatase.

    PubMed

    Kosuga, Motomichi; Mashima, Ryuichi; Hirakiyama, Asami; Fuji, Naoko; Kumagai, Tadayuki; Seo, Joo-Hyun; Nikaido, Mari; Saito, Seiji; Ohno, Kazuki; Sakuraba, Hitoshi; Okuyama, Torayuki

    2016-07-01

    Mucopolysaccharidosis type II (MPS II: also called as Hunter syndrome) is an X-linked recessive lysosomal storage disorder characterized by the accumulation of extracellular glycosaminoglycans due to the deficiency of the enzyme iduronate-2-sulfatase (IDS). Previous observations suggested that MPS II can be classified into two distinct disease subtypes: (1) severe type of MPS II involves a decline in the cognitive ability of a patient and (2) attenuated type of MPS II exhibits no such intellectual phenotype. To determine whether such disease subtypes of MPS II could be explained by genetic diagnosis, we analyzed mutations in the IDS gene of 65 patients suffering from MPS II among the Japanese population who were diagnosed with both the accumulation of urinary glycosaminoglycans and a decrease in their IDS enzyme activity between 2004 and 2014. Among the specimens examined, we identified the following mutations: 33 missense, 8 nonsense, 7 frameshift, 4 intronic changes affecting splicing, 8 recombinations involving IDS-IDS2, and 7 other mutations including 4 large deletions. Consistent with the previous data, the results of our study showed that most of the attenuated phenotype was derived from the missense mutations of the IDS gene, whereas mutations associated with a large structural alteration including recombination, splicing, frameshift, and nonsense mutations were linked to the severe phenotype of MPS II. Furthermore, we conducted a homology modeling study of IDS P120R and N534I mutant as representatives of the causative mutation of the severe and attenuated type of MPS II, respectively. We found that the substitution of P120R of the IDS enzyme was predicted to deform the α-helix generated by I119-F123, leading to the major structural alteration of the wild-type IDS enzyme. In sharp contrast, the effect of the structural alteration of N534I was marginal; thus, this mutation was pathogenically predicted to be associated with the attenuated type of MPS II

  12. Comparison of Perinatal Risk Factors Associated with Autism Spectrum Disorder (ASD), Intellectual Disability (ID), and Co-Occurring ASD and ID

    ERIC Educational Resources Information Center

    Schieve, Laura A.; Clayton, Heather B.; Durkin, Maureen S.; Wingate, Martha S.; Drews-Botsch, Carolyn

    2015-01-01

    While studies report associations between perinatal outcomes and both autism spectrum disorder (ASD) and intellectual disability (ID), there has been little study of ASD with versus without co-occurring ID. We compared perinatal risk factors among 7547 children in the 2006-2010 Autism and Developmental Disability Monitoring Network classified as…

  13. ATF3 suppresses ESCC via downregulation of ID1

    PubMed Central

    Li, Jian; Yang, Zishan; Chen, Zhiuguo; Bao, Yonghua; Zhang, Huijuan; Fang, Xinhui; Yang, Wancai

    2016-01-01

    Esophageal cancer is one of the most prevalent forms of cancer and has a particularly high mortality rate due to early metastasis; however, the underlying mechanisms of its formation and progression remain unclear. The present study performed immunohistochemical analysis and observed that the expression of activating transcription factor 3 (ATF3) was reduced in esophageal squamous cell carcinoma (ESCC) in comparison with non-tumor adjacent tissues. By contrast, inhibitor of DNA binding 1 (ID1) was overexpressed in ESCC tissues, demonstrating an inverse correlation with ATF3 (P<0.01). In ESCC EC109 and KYSE450 cells lines, transfection with an ATF3-overexpression plasmid resulted in the inhibition of cell proliferation, motility and migration, which was associated with the induction of E-cadherin expression and inhibition of cyclin D1 and Twist. Notably, ATF3 exerted an inverse regulatory interaction with ID1. The results of the present study provide additional evidence of the tumor suppressive features of ATF3 and demonstrate a novel mechanism of ATF3-mediated inhibition of cancer metastasis in esophageal cancer. PMID:27602100

  14. Id or subego? Some theoretical questions for clinicians.

    PubMed

    Downey, T W

    1989-01-01

    This paper began with definitions and it will end with one; albeit a tentative one. Subego: The postulated realm of the mind derived from the infant-mother experience culminating in the development or maldevelopment of a partial sense of self as sensate, effective, and autonomous. A structure for driving and interpreting experience through largely unconscious visual imagery, a structure in a state of constant communication and overlap with the ego and superego. Reviewing what I have written leads to this afterword. Perhaps the present conceptualization of the id gives physiology and animal behavior a bad name. Perhaps we would advance structural theory more if we reserved the term "id" for the distorted state of wish fulfillment resulting from compromised parental malnurturing of the "good enough infant." Intrapsychic activities must to some degree be inimical. A better conceptual balance needs to be struck between their harmonious and antagonistic functioning. This condensed preliminary communication was intended to suggest some ways in which, in the spirit of Loewald, experience can give meaning to theory and theory can become a beacon for hardy clinicians seeking new avenues of exploration.

  15. DamID Analysis of Nuclear Organization in Caenorhabditis elegans.

    PubMed

    Gómez-Saldivar, Georgina; Meister, Peter; Askjaer, Peter

    2016-01-01

    The development of genomics and next generation sequencing platforms has dramatically improved our insight into chromatin structure and organization and its fine interplay with gene expression. The nuclear envelope has emerged as a key component in nuclear organization via extensive contacts between the genome and numerous proteins at the nuclear periphery. These contacts may have profound effects on gene expression as well as cell proliferation and differentiation. Indeed, their perturbations are associated with several human pathologies known as laminopathies or nuclear envelopathies. However, due to their dynamic behavior the contacts between nuclear envelope proteins and chromatin are challenging to identify, in particular in intact tissues. Here, we propose the DamID technique as an attractive method to globally characterize chromatin organization in the popular model organism Caenorhabditis elegans. DamID is based on the in vivo expression of a chromatin-associated protein of interest fused to the Escherichia coli DNA adenine methyltransferase, which produces unique identification tags at binding site in the genome. This marking is simple, highly specific and can be mapped by sensitive enzymatic and next generation sequencing approaches.

  16. ATF3 suppresses ESCC via downregulation of ID1

    PubMed Central

    Li, Jian; Yang, Zishan; Chen, Zhiuguo; Bao, Yonghua; Zhang, Huijuan; Fang, Xinhui; Yang, Wancai

    2016-01-01

    Esophageal cancer is one of the most prevalent forms of cancer and has a particularly high mortality rate due to early metastasis; however, the underlying mechanisms of its formation and progression remain unclear. The present study performed immunohistochemical analysis and observed that the expression of activating transcription factor 3 (ATF3) was reduced in esophageal squamous cell carcinoma (ESCC) in comparison with non-tumor adjacent tissues. By contrast, inhibitor of DNA binding 1 (ID1) was overexpressed in ESCC tissues, demonstrating an inverse correlation with ATF3 (P<0.01). In ESCC EC109 and KYSE450 cells lines, transfection with an ATF3-overexpression plasmid resulted in the inhibition of cell proliferation, motility and migration, which was associated with the induction of E-cadherin expression and inhibition of cyclin D1 and Twist. Notably, ATF3 exerted an inverse regulatory interaction with ID1. The results of the present study provide additional evidence of the tumor suppressive features of ATF3 and demonstrate a novel mechanism of ATF3-mediated inhibition of cancer metastasis in esophageal cancer.

  17. Sex-linked dominant

    MedlinePlus

    Inheritance - sex-linked dominant; Genetics - sex-linked dominant; X-linked dominant; Y-linked dominant ... can be either an autosomal chromosome or a sex chromosome. It also depends on whether the trait ...

  18. ID4 promotes AR expression and blocks tumorigenicity of PC3 prostate cancer cells.

    PubMed

    Komaragiri, Shravan Kumar; Bostanthirige, Dhanushka H; Morton, Derrick J; Patel, Divya; Joshi, Jugal; Upadhyay, Sunil; Chaudhary, Jaideep

    2016-09-01

    Deregulation of tumor suppressor genes is associated with tumorigenesis and the development of cancer. In prostate cancer, ID4 is epigenetically silenced and acts as a tumor suppressor. In normal prostate epithelial cells, ID4 collaborates with androgen receptor (AR) and p53 to exert its tumor suppressor activity. Previous studies have shown that ID4 promotes tumor suppressive function of AR whereas loss of ID4 results in tumor promoter activity of AR. Previous study from our lab showed that ectopic ID4 expression in DU145 attenuates proliferation and promotes AR expression suggesting that ID4 dependent AR activity is tumor suppressive. In this study, we examined the effect of ectopic expression of ID4 on highly malignant prostate cancer cell, PC3. Here we show that stable overexpression of ID4 in PC3 cells leads to increased apoptosis and decreased cell proliferation and migration. In addition, in vivo studies showed a decrease in tumor size and volume of ID4 overexpressing PC3 cells, in nude mice. At the molecular level, these changes were associated with increased androgen receptor (AR), p21, and AR dependent FKBP51 expression. At the mechanistic level, ID4 may regulate the expression or function of AR through specific but yet unknown AR co-regulators that may determine the final outcome of AR function.

  19. Library Networks: Trends and Issues.

    ERIC Educational Resources Information Center

    Martin, Susan K.

    1987-01-01

    Discussion of library networking issues that may be relevant to Japanese and U.S. libraries as they increase their use of central databases and local systems includes the role of the traditional library, distributed systems, linked systems, and the emergence of commercial systems. (Author/CLB)

  20. Pipeline issues

    NASA Technical Reports Server (NTRS)

    Eisley, Joe T.

    1990-01-01

    The declining pool of graduates, the lack of rigorous preparation in science and mathematics, and the declining interest in science and engineering careers at the precollege level promises a shortage of technically educated personnel at the college level for industry, government, and the universities in the next several decades. The educational process, which starts out with a large number of students at the elementary level, but with an ever smaller number preparing for science and engineering at each more advanced educational level, is in a state of crisis. These pipeline issues, so called because the educational process is likened to a series of ever smaller constrictions in a pipe, were examined in a workshop at the Space Grant Conference and a summary of the presentations and the results of the discussion, and the conclusions of the workshop participants are reported.

  1. 6 CFR 37.29 - Prohibition against holding more than one REAL ID card or more than one driver's license.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... ID card or more than one driver's license. 37.29 Section 37.29 Domestic Security DEPARTMENT OF HOMELAND SECURITY, OFFICE OF THE SECRETARY REAL ID DRIVER'S LICENSES AND IDENTIFICATION CARDS Minimum... REAL ID card or more than one driver's license. (a) An individual may hold only one REAL ID card....

  2. 6 CFR 37.29 - Prohibition against holding more than one REAL ID card or more than one driver's license.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... ID card or more than one driver's license. 37.29 Section 37.29 Domestic Security DEPARTMENT OF HOMELAND SECURITY, OFFICE OF THE SECRETARY REAL ID DRIVER'S LICENSES AND IDENTIFICATION CARDS Minimum... REAL ID card or more than one driver's license. (a) An individual may hold only one REAL ID card....

  3. Linking to the Future

    NASA Astrophysics Data System (ADS)

    Moore, John W.

    1999-09-01

    my copy of JCE in the mail each month, and I expect you do too. I can glance at the cover to get an overview of an issue's content, and I usually am enticed inside by intriguing cover art. I can scan the table of contents to find articles I want to read, or I can just browse through the issue to see what looks interesting. Usually the editors have juxtaposed related articles so that I often find a small treasure trove. The printed Journal is quite portable and can be read in a car or airplane. It will last a long time, and until the paper deteriorates, I will never have a problem reading back issues. I have almost every issue from the first day I subscribed and have even added some older ones from collections of retired colleagues who no longer had shelf space for them. I certainly would not want to give up my printed copies, and I want to keep getting them. I find that JCE Online provides a different kind of resource that is equally valuable. It contains more information, and information that is more appropriate in electronic form. It links related ideas into a much more complex web of information than is possible in print. And it opens pathways to lots of information that is not part of JCE but resides elsewhere. Using this issue as an example, let's take a tour of what JCE Online can do.

    • Point your Web browser to http://jchemed.chem.wisc.edu
    • Click on Journal and then on Current Issue (unless September 1999 is no longer the current issue, in which case you will find it in Past Issues).
    • In the table of contents, find the article "UV Catalysis, Cyanotype Photography, and Sunscreens". Click on the title.
    • When the abstract appears, click on Full Text (PDF) to see the article, just as it appears on page 1199 in this issue.
    • When you are prompted, enter the name and subscriber number from your address label.
    • At the end of the article you will find that supplementary materials are available (including a procedure for testing

    • Identifier (ID) elements are not preferentially located to brain-specific genes: high ID element representation in other tissue-specific- and housekeeping genes of the rat.

      PubMed

      Goldman, Andrés; Capoano, Carlos A; González-López, Evangelina; Geisinger, Adriana

      2014-01-01

      BC1 is a short non-coding RNA from rodents, which is transcribed by RNA pol III. Its RNA is highly abundant in the brain, where it exerts a post-transcriptional regulatory role in dendrites. Upon transcription, retroposition and insertion, BC1 gives rise to a subclass of short interspersed repetitive sequences (SINEs) named identifier (ID) elements. IDs can become integrated inside non-coding regions of RNA pol II transcription units, and - although challenged by a couple of reports - their preferential location to brain-specific genes has been long proposed. Furthermore, an additional, cis-regulatory role in the control of brain-specific pol II-directed transcripts has been suggested for these sequences. In this work we used Northern blot and in silico analyses to examine IDs' location among pol II transcription units in different tissues, and in housekeeping genes. ID sequences appeared distributed in a similar fashion within tissue-specific hnRNA populations of the brain, testis and liver, and within housekeeping primary transcripts as well. Moreover, when the lengths of the unprocessed transcripts were considered, ID representation was higher in housekeeping ones. On the other hand, ID elements appeared similarly distributed among the different gene regions, with the obvious exclusion of those sequences where strict constraints for proper gene expression exist. Altogether, the widespread distribution of ID elements in all the analyzed genes - including housekeeping - and in all gene regions, suggests a random location, raising questions about the specific cis-regulatory role of those sequences.

    • An improved smaller biotin ligase for BioID proximity labeling

      PubMed Central

      Kim, Dae In; Jensen, Samuel C.; Noble, Kyle A.; KC, Birendra; Roux, Kenneth H.; Motamedchaboki, Khatereh; Roux, Kyle J.

      2016-01-01

      The BioID method uses a promiscuous biotin ligase to detect protein–protein associations as well as proximate proteins in living cells. Here we report improvements to the BioID method centered on BioID2, a substantially smaller promiscuous biotin ligase. BioID2 enables more-selective targeting of fusion proteins, requires less biotin supplementation, and exhibits enhanced labeling of proximate proteins. Thus BioID2 improves the efficiency of screening for protein–protein associations. We also demonstrate that the biotinylation range of BioID2 can be considerably modulated using flexible linkers, thus enabling application-specific adjustment of the biotin-labeling radius. PMID:26912792

    • ID-Check: Online Concealed Information Test Reveals True Identity.

      PubMed

      Verschuere, Bruno; Kleinberg, Bennett

      2016-01-01

      The Internet has already changed people's lives considerably and is likely to drastically change forensic research. We developed a web-based test to reveal concealed autobiographical information. Initial studies identified a number of conditions that affect diagnostic efficiency. By combining these moderators, this study investigated the full potential of the online ID-check. Participants (n = 101) tried to hide their identity and claimed a false identity in a reaction time-based Concealed Information Test. Half of the participants were presented with personal details (e.g., first name, last name, birthday), whereas the others only saw irrelevant details. Results showed that participants' true identity could be detected with high accuracy (AUC = 0.98; overall accuracy: 86-94%). Online memory detection can reliably and validly detect whether someone is hiding their true identity. This suggests that online memory detection might become a valuable tool for forensic applications. PMID:26390033

    • Image processing by Intensity-Dependent Spread (IDS)

      NASA Technical Reports Server (NTRS)

      Cornsweet, Tom N.

      1989-01-01

      As retinal illuminance is lowered, the human visual system integrates the effects of photon absorptions over larger areas and longer times. A theory of the process that might underlie these changes is called Intensity-Dependent Spread (IDS). Each input point gives rise to a pattern of excitation that spreads to a region of the total excitation it sees. The unique aspect of the theory is the assumption that, although the amplitude of the excitation pattern at its center increases with input illuminance, its width decreases in such a way that its volume remains constant. Application of this theory to image processing reveals that it displays a number of unexpected and potentially useful properties. Among them are edge enhancement and independence from scene illumination.

    • ID-Check: Online Concealed Information Test Reveals True Identity.

      PubMed

      Verschuere, Bruno; Kleinberg, Bennett

      2016-01-01

      The Internet has already changed people's lives considerably and is likely to drastically change forensic research. We developed a web-based test to reveal concealed autobiographical information. Initial studies identified a number of conditions that affect diagnostic efficiency. By combining these moderators, this study investigated the full potential of the online ID-check. Participants (n = 101) tried to hide their identity and claimed a false identity in a reaction time-based Concealed Information Test. Half of the participants were presented with personal details (e.g., first name, last name, birthday), whereas the others only saw irrelevant details. Results showed that participants' true identity could be detected with high accuracy (AUC = 0.98; overall accuracy: 86-94%). Online memory detection can reliably and validly detect whether someone is hiding their true identity. This suggests that online memory detection might become a valuable tool for forensic applications.

    • 76 FR 55788 - Amendments To Include New Applicant Types on Form ID

      Federal Register 2010, 2011, 2012, 2013, 2014

      2011-09-09

      ... processing of the form. Form ID is the application for access codes to file on the Commission's Electronic..., 2011), 76 FR 10948 (February 28, 2011) (File No. S7-06-11). \\32\\ Id. \\33\\ See 15 U.S.C. 78c(a)(77) (as... COMMISSION 17 CFR Parts 239, 249, 269 and 274 Amendments To Include New Applicant Types on Form ID...

    • Id-1: Regulator of EGFR and VEGF and potential target for colorectal cancer therapy

      PubMed Central

      Meteoglu, Ibrahim; Meydan, Nezih; Erkus, Muhan

      2008-01-01

      Background The helix-loop-helix transcription factor Id-1 (an inhibitor of differentiation and DNA binding) plays a role in development and progression of many tumours. Id-1 is known to exert its effects on the epidermal growth factor receptor (EGFR) and the vascular endothelial growth factor (VEGF). The aim of this study was to reveal whether there was a relationship between Id-1 and EGFR and VEGF in colorectal carcinoma. Methods Tumour and non-tumour tissue specimens from 46 cases of colorectal carcinoma were exposed to immunohistochemical staining for Id-1, EGFR and VEGF. The relationship between the degree of staining and tumour grade, tumour stage and all tumour markers was investigated. Results Tumour cells showed positive staining for Id-1 in 43 cases (93.5%), for EGFR in 41 cases (89%) and for VEGF in 42 cases (91%). There was a significant relation between the tumour grade and the degree of staining for Id-1, EGFR and VEGF. The relation between the tumour stage and the degree of staining for Id-1, EGFR and VEGF was also significant. There was a significant relation between Id-1 expression and EGFR and VEGF expressions. Non-tumoural tissue specimens were not stained with Id-1 and EGFR antibodies in any of the cases, but stained with VEGF antibody in 3 cases. Conclusion This study revealed that Id-1, EGFR and VEGF took part in development and progression of colorectal carcinomas and that Id-1 was associated with regulations of EGFR and VEGF. The results of this study support the idea that not only EGFR and VEGF but also Id-1 could be new targets in cancer treatment. PMID:19014499

    • The regulation and function of the Id proteins in lymphocyte development.

      PubMed

      Rivera, R; Murre, C

      2001-12-20

      Helix-loop-helix (HLH) proteins are essential factors for lymphocyte development and function. One class of HLH proteins, the E-proteins, regulate many aspects of lymphocyte maturation, survival, proliferation, and differentiation. E-proteins are negatively regulated by another class of HLH proteins known as the Id proteins. The Id proteins function as dominant negative inhibitors of E-proteins by inhibiting their ability to bind DNA. Here we discuss the function and regulation of the Id proteins in lymphocyte development.

    • The Implementation of C-ID, R2D2 Model on Learning Reading Comprehension

      ERIC Educational Resources Information Center

      Rayanto, Yudi Hari; Rusmawan, Putu Ngurah

      2016-01-01

      The purposes of this research are to find out, (1) whether C-ID, R2D2 model is effective to be implemented on learning Reading comprehension, (2) college students' activity during the implementation of C-ID, R2D2 model on learning Reading comprehension, and 3) college students' learning achievement during the implementation of C-ID, R2D2 model on…

    • Underground Storage Tank Integrated Demonstration (UST-ID). Technology summary

      SciTech Connect

      Not Available

      1994-02-01

      The DOE complex currently has 332 underground storage tanks (USTs) that have been used to process and store radioactive and chemical mixed waste generated from weapon materials production. Very little of the over 100 million gallons of high-level and low-level radioactive liquid waste has been treated and disposed of in final form. Two waste storage tank design types are prevalent across the DOE complex: single-shell wall and double-shell wall designs. They are made of stainless steel, concrete, and concrete with carbon steel liners, and their capacities vary from 5000 gallons (19 m{sup 3}) to 10{sup 6} gallons (3785 m{sup 3}). The tanks have an overburden layer of soil ranging from a few feet to tens of feet. Responding to the need for remediation of tank waste, driven by Federal Facility Compliance Agreements (FFCAs) at all participating sites, the Underground Storage Tank Integrated Demonstration (UST-ID) Program was created by the US DOE Office of Technology Development in February 1991. Its mission is to focus the development, testing, and evaluation of remediation technologies within a system architecture to characterize, retrieve, treat to concentrate, and dispose of radioactive waste stored in USTs at DOE facilities. The ultimate goal is to provide safe and cost-effective solutions that are acceptable to the public and the regulators. The UST-ID has focused on five DOE locations: the Hanford Site, which is the host site, in Richland, Washington; the Fernald Site in Fernald, Ohio; the Idaho National Engineering Laboratory near Idaho Falls, Idaho; the Oak Ridge Reservation in Oak Ridge, Tennessee, and the Savannah River Site in Savannah River, South Carolina.

    • DeID - a data sharing tool for neuroimaging studies.

      PubMed

      Song, Xuebo; Wang, James; Wang, Anlin; Meng, Qingping; Prescott, Christian; Tsu, Loretta; Eckert, Mark A

      2015-01-01

      Funding institutions and researchers increasingly expect that data will be shared to increase scientific integrity and provide other scientists with the opportunity to use the data with novel methods that may advance understanding in a particular field of study. In practice, sharing human subject data can be complicated because data must be de-identified prior to sharing. Moreover, integrating varied data types collected in a study can be challenging and time consuming. For example, sharing data from structural imaging studies of a complex disorder requires the integration of imaging, demographic and/or behavioral data in a way that no subject identifiers are included in the de-identified dataset and with new subject labels or identification values that cannot be tracked back to the original ones. We have developed a Java program that users can use to remove identifying information in neuroimaging datasets, while still maintaining the association among different data types from the same subject for further studies. This software provides a series of user interaction wizards to allow users to select data variables to be de-identified, implements functions for auditing and validation of de-identified data, and enables the user to share the de-identified data in a single compressed package through various communication protocols, such as FTPS and SFTP. DeID runs with Windows, Linux, and Mac operating systems and its open architecture allows it to be easily adapted to support a broader array of data types, with the goal of facilitating data sharing. DeID can be obtained at http://www.nitrc.org/projects/deid. PMID:26441500

    • Idaho National Laboratory Supervisory Control and Data Acquisition Intrusion Detection System (SCADA IDS)

      SciTech Connect

      Jared Verba; Michael Milvich

      2008-05-01

      Current Intrusion Detection System (IDS) technology is not suited to be widely deployed inside a Supervisory, Control and Data Acquisition (SCADA) environment. Anomaly- and signature-based IDS technologies have developed methods to cover information technology-based networks activity and protocols effectively. However, these IDS technologies do not include the fine protocol granularity required to ensure network security inside an environment with weak protocols lacking authentication and encryption. By implementing a more specific and more intelligent packet inspection mechanism, tailored traffic flow analysis, and unique packet tampering detection, IDS technology developed specifically for SCADA environments can be deployed with confidence in detecting malicious activity.

    • Id expression in amphioxus and lamprey highlights the role of gene cooption during neural crest evolution

      NASA Technical Reports Server (NTRS)

      Meulemans, Daniel; McCauley, David; Bronner-Fraser, Marianne

      2003-01-01

      Neural crest cells are unique to vertebrates and generate many of the adult structures that differentiate them from their closest invertebrate relatives, the cephalochordates. Id genes are robust markers of neural crest cells at all stages of development. We compared Id gene expression in amphioxus and lamprey to ask if cephalochordates deploy Id genes at the neural plate border and dorsal neural tube in a manner similar to vertebrates. Furthermore, we examined whether Id expression in these cells is a basal vertebrate trait or a derived feature of gnathostomes. We found that while expression of Id genes in the mesoderm and endoderm is conserved between amphioxus and vertebrates, expression in the lateral neural plate border and dorsal neural tube is a vertebrate novelty. Furthermore, expression of lamprey Id implies that recruitment of Id genes to these cells occurred very early in the vertebrate lineage. Based on expression in amphioxus we postulate that Id cooption conferred sensory cell progenitor-like properties upon the lateral neurectoderm, and pharyngeal mesoderm-like properties upon cranial neural crest. Amphioxus Id expression is also consistent with homology between the anterior neurectoderm of amphioxus and the presumptive placodal ectoderm of vertebrates. These observations support the idea that neural crest evolution was driven in large part by cooption of multipurpose transcriptional regulators from other tissues and cell types.

    • Targeting Id protein interactions by an engineered HLH domain induces human neuroblastoma cell differentiation.

      PubMed

      Ciarapica, R; Annibali, D; Raimondi, L; Savino, M; Nasi, S; Rota, R

      2009-04-30

      Inhibitor of DNA-binding (Id) proteins prevent cell differentiation, promote growth and sustain tumour development. They do so by binding to E proteins and other transcription factors through the helix-loop-helix (HLH) domain, and inhibiting transcription. This makes HLH-mediated Id protein interactions an appealing therapeutic target. We have used the dominant interfering HLH dimerization mutant 13I to model the impact of Id inhibition in two human neuroblastoma cell lines: LA-N-5, similar to immature neuroblasts, and SH-EP, resembling more immature precursor cells. We have validated 13I as an Id inhibitor by showing that it selectively binds to Ids, impairs complex formation with RB, and relieves repression of E protein-activated transcription. Id inactivation by 13I enhances LA-N-5 neural features and causes SH-EP cells to acquire neuronal morphology, express neuronal proteins such as N-CAM and NF-160, proliferate more slowly, and become responsive to retinoic acid. Concomitantly, 13I augments the cell-cycle inhibitor p27(Kip1) and reduces the angiogenic factor vascular endothelial growth factor. These effects are Id specific, being counteracted by Id overexpression. Furthermore, 13I strongly impairs tumorigenic properties in agar colony formation and cell invasion assays. Targeting Id dimerization may therefore be effective for triggering differentiation and restraining neuroblastoma cell tumorigenicity.

    • Automatic spent fuel ID-number reader (II) image-processing technique

      SciTech Connect

      Kubo, K.; Mizuguchi, H.; Miyazawa, T. )

      1991-01-01

      This paper reports on an image-processing technique for automatic spent fuel ID-number reader system. As a result, efficiency and reliability of the reader system is improved. To obtain reliable ID-number identification-capability, there are many items to be considered. Some of the important ones are appropriate lighting, high speed processors and suitable algorithm. After developing the technique on the items, the applicability of the image-processing technique for the reader system is verified. ID-numbers are accurately identified, and the time required for one ID-number reading is obtained in about 2 seconds. The result is one tenth of the conventional visual identification.

    • Fake ID ownership and heavy drinking in underage college students: prospective findings.

      PubMed

      Martinez, Julia A; Rutledge, Patricia C; Sher, Kenneth J

      2007-06-01

      The authors examined the ownership of false identification (fake ID) for the purpose of obtaining alcohol and the relation of fake ID ownership to heavy drinking in a longitudinal sample of college students under 21 years of age. A sample of 3,720 undergraduates was assessed the summer prior to college entrance and during the 4 semesters comprising freshman and sophomore years. Regression analyses were used to estimate bidirectional relations between consumption and fake ID ownership. Sex, Greek membership, and prior drinking were controlled. Results showed that fake ID ownership increased over time (12.5% pre-college to 32.2% fourth semester) and that Greek members were more likely than others to own fake IDs. Fake ID ownership predicted concurrent and next-semester heavy drinking with increasing strength over time. Also, the acquisition (onset) of fake ID ownership at each time point was predicted by previous-semester consumption. When traditional, robust risk factors of consumption are controlled, fake ID ownership meaningfully relates to heavy drinking in college. It thus presents a significant public health problem, addressable through training for alcohol servers and retailers, punitive measures toward fake ID owners, and other possible interventions.

    • Intra-domain phage display (ID-PhD) of peptides and protein mini-domains censored from canonical pIII phage display

      PubMed Central

      Tjhung, Katrina F.; Deiss, Frédérique; Tran, Jessica; Chou, Ying; Derda, Ratmir

      2015-01-01

      In this paper, we describe multivalent display of peptide and protein sequences typically censored from traditional N-terminal display on protein pIII of filamentous bacteriophage M13. Using site-directed mutagenesis of commercially available M13KE phage cloning vector, we introduced sites that permit efficient cloning using restriction enzymes between domains N1 and N2 of the pIII protein. As infectivity of phage is directly linked to the integrity of the connection between N1 and N2 domains, intra-domain phage display (ID-PhD) allows for simple quality control of the display and the natural variations in the displayed sequences. Additionally, direct linkage to phage propagation allows efficient monitoring of sequence cleavage, providing a convenient system for selection and evolution of protease-susceptible or protease-resistant sequences. As an example of the benefits of such an ID-PhD system, we displayed a negatively charged FLAG sequence, which is known to be post-translationally excised from pIII when displayed on the N-terminus, as well as positively charged sequences which suppress production of phage when displayed on the N-terminus. ID-PhD of FLAG exhibited sub-nanomolar apparent Kd suggesting multivalent nature of the display. A TEV-protease recognition sequence (TEVrs) co-expressed in tandem with FLAG, allowed us to demonstrate that 99.9997% of the phage displayed the FLAG-TEVrs tandem and can be recognized and cleaved by TEV-protease. The residual 0.0003% consisted of phage clones that have excised the insert from their genome. ID-PhD is also amenable to display of protein mini-domains, such as the 33-residue minimized Z-domain of protein A. We show that it is thus possible to use ID-PhD for multivalent display and selection of mini-domain proteins (Affibodies, scFv, etc.). PMID:25972845

  1. Intra-domain phage display (ID-PhD) of peptides and protein mini-domains censored from canonical pIII phage display.

    PubMed

    Tjhung, Katrina F; Deiss, Frédérique; Tran, Jessica; Chou, Ying; Derda, Ratmir

    2015-01-01

    In this paper, we describe multivalent display of peptide and protein sequences typically censored from traditional N-terminal display on protein pIII of filamentous bacteriophage M13. Using site-directed mutagenesis of commercially available M13KE phage cloning vector, we introduced sites that permit efficient cloning using restriction enzymes between domains N1 and N2 of the pIII protein. As infectivity of phage is directly linked to the integrity of the connection between N1 and N2 domains, intra-domain phage display (ID-PhD) allows for simple quality control of the display and the natural variations in the displayed sequences. Additionally, direct linkage to phage propagation allows efficient monitoring of sequence cleavage, providing a convenient system for selection and evolution of protease-susceptible or protease-resistant sequences. As an example of the benefits of such an ID-PhD system, we displayed a negatively charged FLAG sequence, which is known to be post-translationally excised from pIII when displayed on the N-terminus, as well as positively charged sequences which suppress production of phage when displayed on the N-terminus. ID-PhD of FLAG exhibited sub-nanomolar apparent Kd suggesting multivalent nature of the display. A TEV-protease recognition sequence (TEVrs) co-expressed in tandem with FLAG, allowed us to demonstrate that 99.9997% of the phage displayed the FLAG-TEVrs tandem and can be recognized and cleaved by TEV-protease. The residual 0.0003% consisted of phage clones that have excised the insert from their genome. ID-PhD is also amenable to display of protein mini-domains, such as the 33-residue minimized Z-domain of protein A. We show that it is thus possible to use ID-PhD for multivalent display and selection of mini-domain proteins (Affibodies, scFv, etc.). PMID:25972845

  2. What Health Issues or Conditions Are Specific to Women Only?

    MedlinePlus

    ... Information Clinical Trials Resources and Publications What health issues or conditions are specific to women only? Skip sharing on social media links Share this: Page Content Women experience ...

  3. Ebf1-mediated down-regulation of Id2 and Id3 is essential for specification of the B cell lineage

    PubMed Central

    Thal, Melissa A.; Carvalho, Thiago L.; He, Ti; Kim, Hyung-Gyoon; Gao, Hua; Hagman, James; Klug, Christopher A.

    2009-01-01

    Gene knockout experiments in mice have suggested a hierarchical model of early B cell commitment wherein E2A proteins (E47 and E12) activate early B cell factor (Ebf1), which in turn activates expression of the B cell commitment factor, Pax5. In IL-7 receptor alpha (IL-7Rα) knockout mice, B cell development is blocked before B-lineage commitment at the prepro-B cell stage in adult animals. In IL-7Rα−/− prepro-B cells, E47 is expressed and yet is insufficient to transcriptionally activate the putative downstream target gene, Ebf1. In this study, we show that further increases of E47 expression in IL-7Rα−/− prepro-B cells fails to activate Ebf1, but rather leads to a dramatic induction of the E2A inhibitory factors, Id2 and Id3. In contrast, enforced expression of Ebf1 in IL-7Rα−/− bone marrow potently down-regulates Id2 and Id3 mRNA expression and restores B cell differentiation in vivo. Down-regulation of both Id2 and Id3 during B cell specification is essential in that overexpression of either Id2 or Id3 in wild-type bone marrow blocks B cell specification at the prepro-B cell stage. Collectively, these studies suggest a model where Ebf1 induction specifies the B cell fate by dramatically increasing activity of E47 at the posttranslational level. PMID:19122139

  4. High-speed readout method of ID information on a large amount of electronic tags

    NASA Astrophysics Data System (ADS)

    Nagate, Wataru; Sasabe, Masahiro; Nakano, Hirotaka

    2006-10-01

    An electronic tag such as RFID is expected to create new services that cannot be achieved by the traditional bar code. Specifically, in a distribution system, simultaneous readout method of a large amount of electronic tags embedded in products is required to reduce costs and time. In this paper, we propose novel methods, called Response Probability Control (RPC), to accomplish this requirement. In RPC, a reader firstly sends an ID request to electronic tags in its access area. It succeeds reading information on a tag only if other tags do not respond. To improve the readout efficiency, the reader appropriately controls the response probability in accordance with the number of tags. However, this approach cannot entirely avoid a collision of multiple responses. When a collision occurs, ID information is lost. To reduce the amount of lost data, we divide the ID registration process into two steps. The reader first gathers the former part of the original ID, called temporal ID, according to the above method. After obtaining the temporal ID, it sequentially collects the latter part of ID, called remaining ID, based on the temporal ID. Note that we determine the number of bits of a temporal ID in accordance with the number of tags in the access area so that each tag can be distinguishable. Through simulation experiments, we evaluate RPC in terms of the readout efficiency. Simulation results show that RPC can accomplish the readout efficiency 1.17 times higher than the traditional method where there are a thousand of electronic tags whose IDs are 128 bits.

  5. Id2 and E Proteins Orchestrate the Initiation and Maintenance of MLL-Rearranged Acute Myeloid Leukemia.

    PubMed

    Ghisi, Margherita; Kats, Lev; Masson, Frederick; Li, Jason; Kratina, Tobias; Vidacs, Eva; Gilan, Omer; Doyle, Maria A; Newbold, Andrea; Bolden, Jessica E; Fairfax, Kirsten A; de Graaf, Carolyn A; Firth, Matthew; Zuber, Johannes; Dickins, Ross A; Corcoran, Lynn M; Dawson, Mark A; Belz, Gabrielle T; Johnstone, Ricky W

    2016-07-11

    E proteins and their antagonists, the Id proteins, are transcriptional regulators important for normal hematopoiesis. We found that Id2 acts as a key regulator of leukemia stem cell (LSC) potential in MLL-rearranged acute myeloid leukemia (AML). Low endogenous Id2 expression is associated with LSC enrichment while Id2 overexpression impairs MLL-AF9-leukemia initiation and growth. Importantly, MLL-AF9 itself controls the E-protein pathway by suppressing Id2 while directly activating E2-2 expression, and E2-2 depletion phenocopies Id2 overexpression in MLL-AF9-AML cells. Remarkably, Id2 tumor-suppressive function is conserved in t(8;21) AML. Low expression of Id2 and its associated gene signature are associated with poor prognosis in MLL-rearranged and t(8;21) AML patients, identifying the Id2/E-protein axis as a promising new therapeutic target in AML. PMID:27374225

  6. FHL2 interacts with and acts as a functional repressor of Id2 in human neuroblastoma cells

    PubMed Central

    Han, Weidong; Wu, Zhiqiang; Zhao, Yali; Meng, Yuanguang; Si, Yiling; Yang, Jie; Fu, Xiaobing; Yu, Li

    2009-01-01

    Inhibitor of differentiation 2 (Id2) is a natural inhibitor of the basic helix–loop–helix transcription factors. Although Id2 is well known to prevent differentiation and promote cell-cycle progression and tumorigenesis, the molecular events that regulate Id2 activity remain to be investigated. Here, we identified that Four-and-a-half LIM-only protein 2 (FHL2) is a novel functional repressor of Id2. Moreover, we demonstrated that FHL2 can directly interact with all members of the Id family (Id1–4) via an N-terminal loop–helix structure conserved in Id proteins. FHL2 antagonizes the inhibitory effect of Id proteins on basic helix–loop–helix protein E47-mediated transcription, which was abrogated by the deletion mutation of Ids that disrupted their interaction with FHL2. We also showed a competitive nature between FHL2 and E47 for binding Id2, whereby FHL2 prevents the formation of the Id2–E47 heterodimer, thus releasing E47 to DNA and restoring its transcriptional activity. FHL2 expression was remarkably up-regulated during retinoic acid-induced differentiation of neuroblastoma cells, during which the expression of Id2 was opposite to that. Ectopic FHL2 expression in neuroblastoma cells markedly reduces the transcriptional and cell-cycle promoting functions of Id2. Altogether, these results indicate that FHL2 is an important repressor of the oncogenic activity of Id2 in neuroblastoma cells. PMID:19417068

  7. A rapid and high content assay that measures cyto-ID-stained autophagic compartments and estimates autophagy flux with potential clinical applications.

    PubMed

    Guo, Sujuan; Liang, Yanping; Murphy, Susan F; Huang, Angela; Shen, Haihong; Kelly, Deborah F; Sobrado, Pablo; Sheng, Zhi

    2015-01-01

    The lack of a rapid and quantitative autophagy assay has substantially hindered the development and implementation of autophagy-targeting therapies for a variety of human diseases. To address this critical issue, we developed a novel autophagy assay using the newly developed Cyto-ID fluorescence dye. We first verified that the Cyto-ID dye specifically labels autophagic compartments with minimal staining of lysosomes and endosomes. We then developed a new Cyto-ID fluorescence spectrophotometric assay that makes it possible to estimate autophagy flux based on measurements of the Cyto-ID-stained autophagic compartments. By comparing to traditional autophagy approaches, we found that this assay yielded a more sensitive, yet less variable, quantification of the stained autophagic compartments and the estimate of autophagy flux. Furthermore, we tested the potential application of this autophagy assay in high throughput research by integrating it into an RNA interference (RNAi) screen and a small molecule screen. The RNAi screen revealed WNK2 and MAP3K6 as autophagy-modulating genes, both of which inhibited the MTOR pathway. Similarly, the small molecule screen identified sanguinarine and actinomycin D as potent autophagy inducers in leukemic cells. Moreover, we successfully detected autophagy responses to kinase inhibitors and chloroquine in normal or leukemic mice using this assay. Collectively, this new Cyto-ID fluorescence spectrophotometric assay provides a rapid, reliable quantification of autophagic compartments and estimation of autophagy flux with potential applications in developing autophagy-related therapies and as a test to monitor autophagy responses in patients being treated with autophagy-modulating drugs.

  8. Four hour identification of Enterobacteriaceae with the API Rapid 20E and Micro-ID systems.

    PubMed Central

    Holmes, B; Humphry, P S

    1985-01-01

    One hundred strains of Enterobacteriaceae were examined in parallel with the API Rapid 20E and Micro-ID commercial four hour identification systems. With the API Rapid 20E system 78% of the strains were correctly identified, 15% were not identified, and 7% were misidentified. The respective figures with the Micro-ID system were 74%, 11%, and 15%. PMID:3902898

  9. 39. CALCINER CELL PLANS. TOGETHER WITH HAER ID33C37 ILLUSTRATES COMPLEXITY ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    39. CALCINER CELL PLANS. TOGETHER WITH HAER ID-33-C-37 ILLUSTRATES COMPLEXITY OF PIPING. INEEL DRAWING NUMBER 200-0633-00-287-106445. FLUOR NUMBER 5775-CPP-633-P-50 - Idaho National Engineering Laboratory, Old Waste Calcining Facility, Scoville, Butte County, ID

  10. 40. CALCINER CELL SECTIONS. TOGETHER WITH HAER ID33C37 ILLUSTRATES COMPLEXITY ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    40. CALCINER CELL SECTIONS. TOGETHER WITH HAER ID-33-C-37 ILLUSTRATES COMPLEXITY OF PIPING. INEEL DRAWING NUMBER 200-0633-00-287-106446. FLUOR NUMBER 5775-CPP-P-51. - Idaho National Engineering Laboratory, Old Waste Calcining Facility, Scoville, Butte County, ID

  11. 13. TIP TOP MINE. WOODEN STRUCTURE VISIBLE IN ID31C12 ISIN ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    13. TIP TOP MINE. WOODEN STRUCTURE VISIBLE IN ID-31-C-12 ISIN UPPER LEFT. ALSO VISIBLE ARE TWO VERTICALLY BURIED LOGS ASSOCIATED WITH CABLES, ONE OF WHICH IS VISIBLE IN LOWER RIGHT. CAMERA POINTED SOUTH. - Florida Mountain Mining Sites, Tip Top Mine, West face Florida Mountain, approximately 150 feet below summit, Silver City, Owyhee County, ID

  12. 6 CFR 37.23 - Reissued REAL ID driver's licenses and identification cards.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... identification cards. 37.23 Section 37.23 Domestic Security DEPARTMENT OF HOMELAND SECURITY, OFFICE OF THE SECRETARY REAL ID DRIVER'S LICENSES AND IDENTIFICATION CARDS Minimum Documentation, Verification, and Card Issuance Requirements § 37.23 Reissued REAL ID driver's licenses and identification cards. (a)...

  13. 6 CFR 37.23 - Reissued REAL ID driver's licenses and identification cards.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... identification cards. 37.23 Section 37.23 Domestic Security DEPARTMENT OF HOMELAND SECURITY, OFFICE OF THE SECRETARY REAL ID DRIVER'S LICENSES AND IDENTIFICATION CARDS Minimum Documentation, Verification, and Card Issuance Requirements § 37.23 Reissued REAL ID driver's licenses and identification cards. (a)...

  14. 6 CFR 37.23 - Reissued REAL ID driver's licenses and identification cards.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... identification cards. 37.23 Section 37.23 Domestic Security DEPARTMENT OF HOMELAND SECURITY, OFFICE OF THE SECRETARY REAL ID DRIVER'S LICENSES AND IDENTIFICATION CARDS Minimum Documentation, Verification, and Card Issuance Requirements § 37.23 Reissued REAL ID driver's licenses and identification cards. (a)...

  15. 6 CFR 37.23 - Reissued REAL ID driver's licenses and identification cards.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... SECRETARY REAL ID DRIVER'S LICENSES AND IDENTIFICATION CARDS Minimum Documentation, Verification, and Card Issuance Requirements § 37.23 Reissued REAL ID driver's licenses and identification cards. (a) State... duplicate driver's licenses and identification cards as defined in this rule, the State must...

  16. Documenting the Undocumented: A Review of the United States' First Municipal ID Program

    ERIC Educational Resources Information Center

    Lagunes, Paul F.; Levin, Brian M.; Ditlmann, Ruth K.

    2012-01-01

    The City of New Haven, CT, sought to promote the assimilation of its immigrants by becoming the first American city to provide a government-backed ID, the Elm City Resident Card, to all residents. This article tests the policy's effectiveness in commerce. We find that Hispanics/Latinos are asked to present an ID more than Whites/Anglos, showing…

  17. Id2a is required for hepatic outgrowth during liver development in zebrafish.

    PubMed

    Khaliq, Mehwish; Choi, Tae-Young; So, Juhoon; Shin, Donghun

    2015-11-01

    During development, inhibitor of DNA binding (Id) proteins, a subclass of the helix-loop-helix family of proteins, regulate cellular proliferation, differentiation, and apoptosis in various organs. However, a functional role of Id2a in liver development has not yet been reported. Here, using zebrafish as a model organism, we provide in vivo evidence that Id2a regulates hepatoblast proliferation and cell death during liver development. Initially, in the liver, id2a is expressed in hepatoblasts and after their differentiation, id2a expression is restricted to biliary epithelial cells. id2a knockdown in zebrafish embryos had no effect on hepatoblast specification or hepatocyte differentiation. However, liver size was greatly reduced in id2a morpholino-injected embryos, indicative of a hepatic outgrowth defect attributable to the significant decrease in proliferating hepatoblasts concomitant with the significant increase in hepatoblast cell death. Altogether, these data support the role of Id2a as an important regulator of hepatic outgrowth via modulation of hepatoblast proliferation and survival during liver development in zebrafish.

  18. Wicked ID: Conceptual Framework for Considering Instructional Design as a Wicked Problem

    ERIC Educational Resources Information Center

    Becker, Katrin

    2007-01-01

    The process of instructional design has parallels in other design disciplines. Software design is one that has experienced intense attention in the last 30 or so years, and many lessons learned there can be applied to ID. Using software design as a springboard, this concept paper seeks to propose a new approach to ID. It suggests that…

  19. 77 FR 33794 - True Product ID, Inc.; Order of Suspension of Trading

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-06-07

    ... From the Federal Register Online via the Government Publishing Office SECURITIES AND EXCHANGE COMMISSION True Product ID, Inc.; Order of Suspension of Trading June 5, 2012. It appears to the Securities... securities of True Product ID, Inc. (``True Product'') because it has not filed a periodic report since...

  20. Regulation of Id2 expression by CCAAT/enhancer binding protein β

    PubMed Central

    Karaya, Kazuhiro; Mori, Seiichi; Kimoto, Hisashi; Shima, Yoko; Tsuji, Yoshihito; Kurooka, Hisanori; Akira, Shizuo; Yokota, Yoshifumi

    2005-01-01

    Mice deficient for Id2, a negative regulator of basic helix–loop–helix (bHLH) transcription factors, exhibit a defect in lactation due to impaired lobuloalveolar development during pregnancy, similar to the mice lacking the CCAAT enhancer binding protein (C/EBP) β. Here, we show that Id2 is a direct target of C/EBPβ. Translocation of C/EBPβ into the nucleus, which was achieved by using a system utilizing the fusion protein between C/EBPβ and the ligand-binding domain of the human estrogen receptor (C/EBPβ-ERT), demonstrated the rapid induction of endogenous Id2 expression. In reporter assays, transactivation of the Id2 promoter by C/EBPβ was observed and, among three potential C/EBPβ binding sites found in the 2.3 kb Id2 promoter region, the most proximal element was responsible for the transactivation. Electrophoretic mobility shift assay (EMSA) identified this element as a core sequence to which C/EBPβ binds. Chromatin immunoprecipitation (ChIP) furthermore confirmed the presence of C/EBPβ in the Id2 promoter region. Northern blotting showed that Id2 expression in C/EBPβ-deficient mammary glands was reduced at 10 days post coitus (d.p.c.), compared with that in wild-type mammary glands. Thus, our data demonstrate that Id2 is a direct target of C/EBPβ and provide insight into molecular mechanisms underlying mammary gland development during pregnancy. PMID:15809228

  1. Role of Id proteins in B lymphocyte activation: new insights from knockout mouse studies.

    PubMed

    Sugai, Manabu; Gonda, Hiroyuki; Nambu, Yukiko; Yokota, Yoshifumi; Shimizu, Akira

    2004-09-01

    Id (inhibitor of differentiation) proteins play important roles in cell differentiation, cell cycle control, and apoptosis. They act as negative regulators of basic helix-loop-helix-type transcription factors, which positively regulate differentiation of various cell types. Id proteins work to block B lymphocyte (B cell) maturation at an early differentiation step, as demonstrated by gain-of-function studies. In recent years a series of gene-targeted mice lacking different Ids have been generated. Analyses of these gene-targeted mice provide information useful for understanding the physiological roles of Ids in B cell biology. Id3 is required for proper B cell functions and acts by controlling the cell cycle. Upon B cell activation, Id2 acts as a negative regulator to prevent potentially harmful effects brought about by excessive immunological reactions; one of its special roles is to maintain low serum concentrations of immunoglobulin E (IgE). The Id2 protein does this by antagonizing E2A and Pax5 activities, both of which are required for proper B cell activation. This review presents several new insights into B cell differentiation and activation programs and the physiological role of Id proteins in B cell activation. PMID:15184986

  2. Rethinking Social Network Assessment for Students with Intellectual Disabilities (ID) in Postsecondary Education

    ERIC Educational Resources Information Center

    Eisenman, Laura T.; Farley-Ripple, Elizabeth; Culnane, Mary; Freedman, Brian

    2013-01-01

    Social networks of persons with intellectual disabilities (ID) have been characterized as smaller and less diverse than those of typical peers. Advocates have focused on strengthening those social networks by expanding circles of social support, protection, and friendship. As young adults with ID experience increasing levels of community…

  3. Security and Privacy Improvements for the Belgian eID Technology

    NASA Astrophysics Data System (ADS)

    Verhaeghe, Pieter; Lapon, Jorn; de Decker, Bart; Naessens, Vincent; Verslype, Kristof

    The Belgian Electronic Identity Card enables Belgian citizens to prove their identity digitally and to sign electronic documents. At the end of 2009, every Belgian citizen older than 12 years will have such an eID card. In the future, usage of the eID card may be mandatory. However, irresponsible use of the card may cause harm to individuals.

  4. An ID Network System to Prepare for Global Environmental/Health Concerns

    NASA Astrophysics Data System (ADS)

    Asano, Shoichiro; Yoneda, Susumu

    Climate change and/or pandemics are global life threatening concerns. For verifying and utilizing monitored data for solving to the Climate Change concerns, a network system based on device ID would be proposed. In this paper, we review the recent standardization initiatives in ITU-T, and propose an ID network that can be used to verify the solutions.

  5. G-Links: a gene-centric link acquisition service

    PubMed Central

    Oshita, Kazuki; Tomita, Masaru; Arakawa, Kazuharu

    2015-01-01

    With the availability of numerous curated databases, researchers are now able to efficiently use the multitude of biological data by integrating these resources via hyperlinks and cross-references. A large proportion of bioinformatics research tasks, however, may include labor-intensive tasks such as fetching, parsing, and merging datasets and functional annotations from distributed multi-domain databases. This data integration issue is one of the key challenges in bioinformatics. We aim to provide an identifier conversion and data aggregation system as a part of solution to solve this problem with a service named G-Links, 1) by gathering resource URI information from 130 databases and 30 web services in a gene-centric manner so that users can retrieve all available links about a given gene, 2) by providing RESTful API for easy retrieval of links including facet searching based on keywords and/or predicate types, and 3) by producing a variety of outputs as visual HTML page, tab-delimited text, and in Semantic Web formats such as Notation3 and RDF. G-Links as well as other relevant documentation are available at http://link.g-language.org/ PMID:26673001

  6. Boundary issues

    NASA Astrophysics Data System (ADS)

    Townsend, Alan R.; Porder, Stephen

    2011-03-01

    A 2008 Transformation of the nitrogen cycle: Recent trends, questions, and potential solutions Science 320 889-92 Handoh I C and Lenton T M 2003 Periodic mid-Cretaceous oceanic anoxic events linked by oscillations of the phosphorus and oxygen biogeochemical cycles global Biogeochemical Cycles 17 1092 MacDonald G K, Bennett E M, Potter P A and Ramankutty N 2011 Agronomic phosphorus imbalances across the world_s croplands Proc. Natl Acad. Sci. 108 3086-91 Malthus T 1798 An Essay on the Principle of Population (available at http://www.gutenberg.org/browse/authors/m\\#a1411 Smil V 2000 Phosphorus in the environment: Natural flows and human interferences Annu. Rev. Energy Environ. 25 53-88 Rockström J et al 2009 A safe operating space for humanity Nature 461 472-5 Sanchez P A and Swaminathan M S 2005 Cutting world hunger in half Science 307 357-9 Schlesinger W H 2009 Planetary boundaries: Thresholds risk prolonged degradation Nature Reports Climate Change doi:10.1038/climate.2009.93 Smith V H and Schindler D W 2009 Eutrophication science: where do we go from here? Trends in Ecology and Evolution 24 201-7 Van Kauenbergh S J 2010 World Phosphate Rock Reserves and Resources (Muscle Shoals, AL: International Fertilizer Development Center) Vitousek P M, Naylor R, Crews T, David M B, Drinkwater L E, Holland E, Johnes P J, Katzenberger J, Martinelli L A, Matson P A, Nziguheba G, Ojima D, Palm C A, Robertson G P, Sanchez P A, Townsend A R, Zhang F S 2009 Nutrient Imbalances in Agricultural Development Science 324 1519-20

  7. On the security flaws in ID-based password authentication schemes for telecare medical information systems.

    PubMed

    Mishra, Dheerendra

    2015-01-01

    Telecare medical information systems (TMIS) enable healthcare delivery services. However, access of these services via public channel raises security and privacy issues. In recent years, several smart card based authentication schemes have been introduced to ensure secure and authorized communication between remote entities over the public channel for the (TMIS). We analyze the security of some of the recently proposed authentication schemes of Lin, Xie et al., Cao and Zhai, and Wu and Xu's for TMIS. Unfortunately, we identify that these schemes failed to satisfy desirable security attributes. In this article we briefly discuss four dynamic ID-based authentication schemes and demonstrate their failure to satisfy desirable security attributes. The study is aimed to demonstrate how inefficient password change phase can lead to denial of server scenario for an authorized user, and how an inefficient login phase causes the communication and computational overhead and decrease the performance of the system. Moreover, we show the vulnerability of Cao and Zhai's scheme to known session specific temporary information attack, vulnerability of Wu and Xu's scheme to off-line password guessing attack, and vulnerability of Xie et al.'s scheme to untraceable on-line password guessing attack. PMID:25486891

  8. On the security flaws in ID-based password authentication schemes for telecare medical information systems.

    PubMed

    Mishra, Dheerendra

    2015-01-01

    Telecare medical information systems (TMIS) enable healthcare delivery services. However, access of these services via public channel raises security and privacy issues. In recent years, several smart card based authentication schemes have been introduced to ensure secure and authorized communication between remote entities over the public channel for the (TMIS). We analyze the security of some of the recently proposed authentication schemes of Lin, Xie et al., Cao and Zhai, and Wu and Xu's for TMIS. Unfortunately, we identify that these schemes failed to satisfy desirable security attributes. In this article we briefly discuss four dynamic ID-based authentication schemes and demonstrate their failure to satisfy desirable security attributes. The study is aimed to demonstrate how inefficient password change phase can lead to denial of server scenario for an authorized user, and how an inefficient login phase causes the communication and computational overhead and decrease the performance of the system. Moreover, we show the vulnerability of Cao and Zhai's scheme to known session specific temporary information attack, vulnerability of Wu and Xu's scheme to off-line password guessing attack, and vulnerability of Xie et al.'s scheme to untraceable on-line password guessing attack.

  9. Ubiquitin-SUMO circuitry controls activated fanconi anemia ID complex dosage in response to DNA damage.

    PubMed

    Gibbs-Seymour, Ian; Oka, Yasuyoshi; Rajendra, Eeson; Weinert, Brian T; Passmore, Lori A; Patel, Ketan J; Olsen, Jesper V; Choudhary, Chunaram; Bekker-Jensen, Simon; Mailand, Niels

    2015-01-01

    We show that central components of the Fanconi anemia (FA) DNA repair pathway, the tumor suppressor proteins FANCI and FANCD2 (the ID complex), are SUMOylated in response to replication fork stalling. The ID complex is SUMOylated in a manner that depends on the ATR kinase, the FA ubiquitin ligase core complex, and the SUMO E3 ligases PIAS1/PIAS4 and is antagonized by the SUMO protease SENP6. SUMOylation of the ID complex drives substrate selectivity by triggering its polyubiquitylation by the SUMO-targeted ubiquitin ligase RNF4 to promote its removal from sites of DNA damage via the DVC1-p97 ubiquitin segregase complex. Deregulation of ID complex SUMOylation compromises cell survival following replication stress. Our results uncover a regulatory role for SUMOylation in the FA pathway, and we propose that ubiquitin-SUMO signaling circuitry is a mechanism that contributes to the balance of activated ID complex dosage at sites of DNA damage.

  10. Overestimation of the 25(OH)D serum concentration with the automated IDS EIA kit.

    PubMed

    Cavalier, Etienne; Huberty, Véronique; Cormier, Catherine; Souberbielle, Jean-Claude

    2011-02-01

    We have recently observed an increasing number of patients presenting very high serum levels of 25-hydroxyvitamin D [25(OH)D] (> 150 ng/mL), which, in all cases, had been measured with the IDS EIA kit adapted on different "open" automated platforms. We performed a comparison between the IDS EIA kit adapted on two different "open"automated platforms and the DiaSorin RIA. We found a systematic bias (higher levels with the IDS EIA kit) for concentrations more than 50-60 ng/mL that was less obvious when the IDS EIA was used in its manual procedure. We thus suggest to use the IDS EIA kit in its manual procedure rather than to adapt it on an automated platform, and to interpret cautiously a 25(OH)D greater than 100 ng/mL with this kit.

  11. IDS crossing of the blood-brain barrier corrects CNS defects in MPSII mice.

    PubMed

    Polito, Vinicia Assunta; Cosma, Maria Pia

    2009-08-01

    Mucopolysaccharidosis type II (MPSII), or Hunter syndrome, arises from a deficiency in iduronate 2-sulfatase (IDS), and it is characterized by progressive somatic and neurological involvement. The MPSII mouse model reproduces the features of MPSII patients. Systemic administration of the AAV2/5CMV-hIDS vector in MPSII mouse pups results in the full correction of glycosaminoglycan (GAG) accumulation in visceral organs and in the rescue of the defects and GAG accumulation in the central nervous system (CNS). Remarkably, in treated MPSII animals, this CNS correction arises from the crossing of the blood-brain barrier by the IDS enzyme itself, not from the brain transduction. Thus, we show here that early treatment of MPSII mice with one systemic injection of AAV2/5CMV-hIDS results in prolonged and high levels of circulating IDS that can efficiently and simultaneously rescue both visceral and CNS defects for up to 18 months after therapy.

  12. Thymocyte selection is regulated by the helix-loop-helix inhibitor protein, Id3.

    PubMed

    Rivera, R R; Johns, C P; Quan, J; Johnson, R S; Murre, C

    2000-01-01

    E2A, HEB, E2-2, and daughterless are basic helix-loop-helix (bHLH) proteins that play key roles in multiple developmental pathways. The DNA binding activity of E2A, HEB, and E2-2 is regulated by a distinct class of inhibitor HLH proteins, the Id gene products. Here, we show that Id3 is required for major histocompatability (MHC) class I- and class II-restricted thymocyte positive selection. Additionally, H-Y TCR-mediated negative selection is severely perturbed in Id3 null mutant mice. Finally, we show that E2A and Id3 interact genetically to regulate thymocyte development. These observations identify the HLH inhibitory protein Id3 as an essential component required for proper thymocyte maturation.

  13. Molecular characterization of lactobacilli isolated from fermented idli batter

    PubMed Central

    Agaliya, Perumal Jayaprabha; Jeevaratnam, Kadirvelu

    2013-01-01

    Lactic acid bacteria are non pathogenic organism widely distributed in nature typically involved in a large number of spontaneous food fermentation. The purpose of this study was to characterize the bacteriocinogenic lactobacilli from fermented idli batter which can find application in biopreservation and biomedicine. Eight most promising lactobacilli were chosen from twenty two isolates based on their spectrum of activity against other lactic acid bacteria and pathogens. The eight lactobacilli were characterized based on the various classical phenotypic tests, physiological tests and biochemical tests including various carbohydrate utilization profiles. All isolates were homo fermentative, catalase, and gelatin negative. Molecular characterization was performed by RAPD, 16S rRNA analysis, 16S ARDRA, and Multiplex PCR for species identification. RAPD was carried out using the primer R2 and M13. Five different clusters were obtained based on RAPD indicating strain level variation. 16S rRNA analysis showed 99 to 100% homology towards Lactobacillus plantarum. The restriction digestion pattern was similar for all the isolates with the restriction enzyme AluI. The subspecies were identified by performing Multiplex PCR using species specific primer. Among the five clusters, three clusters were clearly identified as Lactobacillus plantarum subsp. plantarum, Lactobacillus pentosus, and Lactobacillus plantarum subsp. argentoratensis. PMID:24688512

  14. A neural-visualization IDS for honeynet data.

    PubMed

    Herrero, Álvaro; Zurutuza, Urko; Corchado, Emilio

    2012-04-01

    Neural intelligent systems can provide a visualization of the network traffic for security staff, in order to reduce the widely known high false-positive rate associated with misuse-based Intrusion Detection Systems (IDSs). Unlike previous work, this study proposes an unsupervised neural models that generate an intuitive visualization of the captured traffic, rather than network statistics. These snapshots of network events are immensely useful for security personnel that monitor network behavior. The system is based on the use of different neural projection and unsupervised methods for the visual inspection of honeypot data, and may be seen as a complementary network security tool that sheds light on internal data structures through visual inspection of the traffic itself. Furthermore, it is intended to facilitate verification and assessment of Snort performance (a well-known and widely-used misuse-based IDS), through the visualization of attack patterns. Empirical verification and comparison of the proposed projection methods are performed in a real domain, where two different case studies are defined and analyzed.

  15. Comparison of ID performance using real and synthetic imagery

    NASA Astrophysics Data System (ADS)

    Jacobs, Eddie L.; Edwards, Timothy C.; Miller, Brian; Hodgkin, Van A.

    2002-07-01

    Recent experiments performed at the U.S. Army Night Vision and Electronic Sensors Directorate (NVESD) provide significant insight into the validation of synthetic imagery for use in human perception experiments. This paper documents the procedures and results of target identification (ID) experiments using real and synthetic thermal imagery. Real imagery representing notional first generation and advanced scanning sensor systems was obtained. Parameters derived from the sensor data were used to generate synthetic imagery using the NVESD Paint the Night simulation. Both image sets were then used in a target identification experiment with trained human observers. Perception test results were analyzed and compared with metrics derived from the imagery. Several parameters missing from the original truth data were found to correlate with differences in the perception data. Synthetic data were regenerated using these additional parameters. A subsequent perception experiment confirmed the importance of these parameters, and a good match was obtained between real and synthetic imagery. While the techniques used in this series of experiments do not constitute a definitive method for validating synthetic imagery, they point to some important observations on validation. The main observation is that both target and local background characteristics must be sufficiently specified in the truth data in order to obtain good agreement between synthetic and real data. The paper concludes with suggestions as to the level of detail necessary for truth data when using synthetic imagery in perception experiments.

  16. Molecular characterization of lactobacilli isolated from fermented idli batter.

    PubMed

    Agaliya, Perumal Jayaprabha; Jeevaratnam, Kadirvelu

    2013-12-01

    Lactic acid bacteria are non pathogenic organism widely distributed in nature typically involved in a large number of spontaneous food fermentation. The purpose of this study was to characterize the bacteriocinogenic lactobacilli from fermented idli batter which can find application in biopreservation and biomedicine. Eight most promising lactobacilli were chosen from twenty two isolates based on their spectrum of activity against other lactic acid bacteria and pathogens. The eight lactobacilli were characterized based on the various classical phenotypic tests, physiological tests and biochemical tests including various carbohydrate utilization profiles. All isolates were homo fermentative, catalase, and gelatin negative. Molecular characterization was performed by RAPD, 16S rRNA analysis, 16S ARDRA, and Multiplex PCR for species identification. RAPD was carried out using the primer R2 and M13. Five different clusters were obtained based on RAPD indicating strain level variation. 16S rRNA analysis showed 99 to 100% homology towards Lactobacillus plantarum. The restriction digestion pattern was similar for all the isolates with the restriction enzyme AluI. The subspecies were identified by performing Multiplex PCR using species specific primer. Among the five clusters, three clusters were clearly identified as Lactobacillus plantarum subsp. plantarum, Lactobacillus pentosus, and Lactobacillus plantarum subsp. argentoratensis.

  17. Six steps in quality intervention development (6SQuID)

    PubMed Central

    Wight, Daniel; Wimbush, Erica; Jepson, Ruth; Doi, Lawrence

    2016-01-01

    Improving the effectiveness of public health interventions relies as much on the attention paid to their design and feasibility as to their evaluation. Yet, compared to the vast literature on how to evaluate interventions, there is little to guide researchers or practitioners on how best to develop such interventions in practical, logical, evidence based ways to maximise likely effectiveness. Existing models for the development of public health interventions tend to have a strong social-psychological, individual behaviour change orientation and some take years to implement. This paper presents a pragmatic guide to six essential Steps for Quality Intervention Development (6SQuID). The focus is on public health interventions but the model should have wider applicability. Once a problem has been identified as needing intervention, the process of designing an intervention can be broken down into six crucial steps: (1) defining and understanding the problem and its causes; (2) identifying which causal or contextual factors are modifiable: which have the greatest scope for change and who would benefit most; (3) deciding on the mechanisms of change; (4) clarifying how these will be delivered; (5) testing and adapting the intervention; and (6) collecting sufficient evidence of effectiveness to proceed to a rigorous evaluation. If each of these steps is carefully addressed, better use will be made of scarce public resources by avoiding the costly evaluation, or implementation, of unpromising interventions. PMID:26573236

  18. Minimum wafer thickness by rotated ingot ID wafering. [Inner Diameter

    NASA Technical Reports Server (NTRS)

    Chen, C. P.; Leipold, M. H.

    1984-01-01

    The efficient utilization of materials is critical to certain device applications such as silicon for photovoltaics or diodes and gallium-gadolinium-garnet for memories. A variety of slicing techniques has been investigated to minimize wafer thickness and wafer kerf. This paper presents the results of analyses of ID wafering of rotated ingots based on predicted fracture behavior of the wafer as a result of forces during wafering and the properties of the device material. The analytical model indicated that the minimum wafer thickness is controlled by the depth of surface damage and the applied cantilever force. Both of these factors should be minimized. For silicon, a minimum thickness was found to be approximately 200 x 10 - 6th m for conventional sizes of rotated ingot wafering. Fractures through the thickness of the wafer rather than through the center supporting column were found to limit the minimum wafer thickness. The model suggested that the use of a vacuum chuck on the wafer surface to enhance cleavage fracture of the center supporting core and, with silicon, by using 111-line-type ingots could have potential for reducing minimum wafer thickness.

  19. Pros and Cons of ID vs. 3D Modeling

    NASA Technical Reports Server (NTRS)

    Klimchuk, James A.

    2012-01-01

    Advances in computing capability have led to tremendous improvements in 3D modeling. Entire active regions are being simulated in what might be described as a first principles way, in which plasma heating is treated self consistently rather than through the specification of heating functions. There are limitations to this approach, however, as actual heating mechanisms on the Sun involve spatial scales orders of magnitude smaller than what these simulations can resolve. Other simulations begin to resolve these scales, but they only treat a tiny volume and do not include the all important coupling with larger scales or with other parts of the atmosphere, and so cannot be readily compared with observations. Finally, ID hydrodynamic models capture the field-aligned evolution of the plasma extremely well and are ideally suited for data comparison, but they treat the heating in a totally ad hoc manner. All of these approaches have important contributions to make, but we must be aware of their limitations. I will highlight some of the strengths. and weaknesses of each.

  20. Protocol for VOC-Arid ID remediation performance characterization

    SciTech Connect

    Tegner, B.J.; Hassig, N.L.; Last, G.V.

    1994-09-01

    The Volatile Organic Compound-Arid Integrated Demonstration (VOC-Arid ID) is a technology development program sponsored by the US Department of Energy`s Office of Technology Development that is targeted to acquire, develop, demonstrate, and deploy new technologies for the remediation of VOC contaminants in the soils and groundwaters of arid DOE sites. Technologies cannot be adequately evaluated unless sufficient site characterization and technology performance data have been collection and analyzed. The responsibility for identifying these data needs has been placed largely on the Principal Investigators (PIs) developing the remediation technology, who usually are not experts in site characterization or in identification of appropriate sampling, analysis, and monitoring techniques to support the field testing. This document provides a protocol for planning the collection of data before, during, and after a test of a new technology. This generic protocol provides the PIs and project managers with a set of steps to follow. The protocol is based on a data collection planning process called the Data Quality Objectives (DQO) process, which was originally developed by the US Environmental Protection Agency and has been expanded by DOE to support site cleanup decisions. The DQO process focuses on the quality and quantity of data required to make decision. Stakeholders to the decisions must negotiate such key inputs to the process as the decision rules that will be used and the acceptable probabilities of making decision errors.

  1. Molecular characterization of lactobacilli isolated from fermented idli batter.

    PubMed

    Agaliya, Perumal Jayaprabha; Jeevaratnam, Kadirvelu

    2013-12-01

    Lactic acid bacteria are non pathogenic organism widely distributed in nature typically involved in a large number of spontaneous food fermentation. The purpose of this study was to characterize the bacteriocinogenic lactobacilli from fermented idli batter which can find application in biopreservation and biomedicine. Eight most promising lactobacilli were chosen from twenty two isolates based on their spectrum of activity against other lactic acid bacteria and pathogens. The eight lactobacilli were characterized based on the various classical phenotypic tests, physiological tests and biochemical tests including various carbohydrate utilization profiles. All isolates were homo fermentative, catalase, and gelatin negative. Molecular characterization was performed by RAPD, 16S rRNA analysis, 16S ARDRA, and Multiplex PCR for species identification. RAPD was carried out using the primer R2 and M13. Five different clusters were obtained based on RAPD indicating strain level variation. 16S rRNA analysis showed 99 to 100% homology towards Lactobacillus plantarum. The restriction digestion pattern was similar for all the isolates with the restriction enzyme AluI. The subspecies were identified by performing Multiplex PCR using species specific primer. Among the five clusters, three clusters were clearly identified as Lactobacillus plantarum subsp. plantarum, Lactobacillus pentosus, and Lactobacillus plantarum subsp. argentoratensis. PMID:24688512

  2. Links, lecturing and learning: some issues for geoscience education

    NASA Astrophysics Data System (ADS)

    Castleford, John

    1998-08-01

    If the educational potential of the World Wide Web is to be realised, academics need to move beyond the flowery hype from chattering cybersages expatiating on its vast informational potential. As if you had not noticed, the Web is now a bed we share with vast numbers of anorak wearers, get-rich-quick marketers, porn barons, cauliflower-diet promoters and the like. If academics are to be able to educate students on how to use the Web it will need more than pointing them toward a list of "my favorite sites". In this essay I offer a few ideas about how Geoscientists can look past the hype surrounding the World Wide Web and develop a working perspective on its use as a resource to support and reinforce learning.

  3. Planetary protection issues linked to human missions to Mars

    NASA Astrophysics Data System (ADS)

    Debus, A.

    According to United Nations Treaties and handled presently by the Committee of Space Research COSPAR the exploration of the Solar System has to comply with planetary protection requirements The goal of planetary protection is to protect celestial bodies from terrestrial contamination and also to protect the Earth environment from an eventual biocontamination carried by return samples or by space systems returning to the Earth Mars is presently one of the main target at exobiology point of view and a lot of missions are operating on travel or scheduled for its exploration Some of them include payload dedicated to the search of life or traces of life and one of the goals of these missions is also to prepare sample return missions with the ultimate objective to walk on Mars Robotic missions to Mars have to comply with planetary protection specifications well known presently and planetary protection programs are implemented with a very good reliability taking into account an experience of 40 years now For sample return missions a set of stringent requirements have been approved by the COSPAR and technical challenges have now to be won in order to preserve Earth biosphere from an eventual contamination risk Sending astronauts on Mars will gather all these constraints added with the human dimension of the mission The fact that the astronauts are huge contamination sources for Mars and that they are also potential carrier of a contamination risk back to Earth add also ethical considerations to be considered For the preparation of a such

  4. The Link between Body Issues and Behavioral Problems.

    ERIC Educational Resources Information Center

    Whitehead, James R.; Hoover, John H.

    2000-01-01

    This article highlights the myriad behavioral and adjustment problems that flow from negative self-perceptions about body image in children and adolescents. These negative beliefs are often exacerbated by peer harassment. (Author/MKA)

  5. Resolution of endodontic issues linked to complex anatomy.

    PubMed

    Migliau, Guido; Pepla, Erlind; Besharat, Laith Kostantinos; Gallottini, Livio

    2014-01-01

    Anatomical abnormalities of the root canal system are frequently seen in specialist endodontic practice, and represent a challenge to be faced with skill and thoroughness, beginning with an accurate diagnostic phase and devising the most appropriate treatment plan. Fortunately, much progress has been made in endodontic research thanks to technological advances and the evolution of higher performance instruments, which now consent even very complex cases to be resolved with relative ease. Below are described the salient features of recent progress in endodontics, along with a description of several clinical cases in which the operator has encountered numerous difficulties due to peculiar tooth morphology, overcome successfully thanks to the application of modern tools and consolidated clinical experience in the field.

  6. Influence of ACE I/D Polymorphism on Circulating Levels of Plasminogen Activator Inhibitor 1, D-Dimer, Ultrasensitive C-Reactive Protein and Transforming Growth Factor β1 in Patients Undergoing Hemodialysis

    PubMed Central

    de Carvalho, Sara Santos; Simões e Silva, Ana Cristina; Sabino, Adriano de Paula; Evangelista, Fernanda Cristina Gontijo; Gomes, Karina Braga; Dusse, Luci Maria SantAna; Rios, Danyelle Romana Alves

    2016-01-01

    Background There is substantial evidence that chronic renal and cardiovascular diseases are associated with coagulation disorders, endothelial dysfunction, inflammation and fibrosis. Angiotensin-Converting Enzyme Insertion/Deletion polymorphism (ACE I/D polymorphism) has also be linked to cardiovascular diseases. Therefore, this study aimed to compare plasma levels of ultrassensible C-reactive protein (usCRP), PAI-1, D-dimer and TGF-β1 in patients undergoing HD with different ACE I/D polymorphisms. Methods The study was performed in 138 patients at ESRD under hemodialysis therapy for more than six months. The patients were divided into three groups according to the genotype. Genomic DNA was extracted from blood cells (leukocytes). ACE I/D polymorphism was investigated by single polymerase chain reaction (PCR). Plasma levels of D-dimer, PAI-1 and TGF-β1 were measured by enzyme-linked immunosorbent assay (ELISA), and the determination of plasma levels of usCRP was performed by immunonephelometry. Data were analyzed by the software SigmaStat 2.03. Results Clinical characteristics were similar in patients with these three ACE I/D polymorphisms, except for interdialytic weight gain. I allele could be associated with higher interdialytic weight gain (P = 0.017). Patients genotyped as DD and as ID had significantly higher levels of PAI-1 than those with II genotype. Other laboratory parameters did not significantly differ among the three subgroups (P = 0.033). Despite not reaching statistical significance, plasma levels of usCRP were higher in patients carrying the D allele. Conclusion ACE I/D polymorphisms could be associated with changes in the regulation of sodium, fibrinolytic system, and possibly, inflammation. Our data showed that high levels of PAI-1 are detected when D allele is present, whereas greater interdialytic gain is associated with the presence of I allele. However, further studies with different experimental designs are necessary to elucidate the

  7. Serodiagnosis of human and animal pythiosis using an enzyme-linked immunosorbent assay.

    PubMed Central

    Mendoza, L; Kaufman, L; Mandy, W; Glass, R

    1997-01-01

    Conventional serodiagnosis of Pythium insidiosum infections involves the use of the immunodiffusion (ID) test. This test specifically diagnoses human and animal pythiosis. The test, however, has limited sensitivity and does not detect some culturally proven cases of the disease. Because of the increased recognition of pythiosis among humans and animals, we developed and evaluated an enzyme-linked immunosorbent assay (ELISA) using a soluble antigen from broken hyphae of P. insidiosum. Studies were carried out with sera from five humans and eight animals with culturally and/or histologically proven pythiosis. Some of these sera were negative in the ID test for pythiosis. Heterologous case sera from thirteen humans and two horses, plus 5 sera from healthy humans and 17 from healthy animals, were tested. Of the pythiosis case sera tested, the ID test detected only 8 of 13 (61.5%), whereas the ELISA detected all of them (100%). The ID and ELISA tests were entirely specific and gave negative results or low titers respectively, with sera from humans and animals with heterologous fungal infections or with no apparent illness. No correlation was found between the height of the ELISA titers and negative or positive sera in the ID test. Our results indicate that the ELISA is a reliable serodiagnostic test for pythiosis. It is as specific as the ID test but more sensitive. PMID:9384295

  8. Analyzing Optical Communications Links

    NASA Technical Reports Server (NTRS)

    Marshall, William K.; Burk, Brian D.

    1990-01-01

    Optical Communication Link Analysis Program, OPTI, analyzes optical and near-infrared communication links using pulse-position modulation (PPM) and direct detention. Link margins and design-control tables generated from input parameters supplied by user. Enables user to save sets of input parameters that define given link and read them back into program later. Alters automatically any of input parameters to achieve desired link margin. Written in FORTRAN 77.

  9. Id1 and Id3 co-expression correlates with clinical outcome in stage III-N2 non-small cell lung cancer patients treated with definitive chemoradiotherapy

    PubMed Central

    2013-01-01

    Background Inhibitor of DNA binding 1 (Id1) and 3 (Id3) genes have been related with the inhibition of cell differentiation, cell growth promotion and tumor metastasis. Recently, Id1 has been identified as an independent prognostic factor in patients with lung adenocarcinoma, regardless of the stage. Furthermore, Id1 may confer resistance to treatment (both, radiotherapy and chemotherapy). Methods We have studied, using monoclonal antibodies for immunohistochemistry, the Id1 and Id3 tumor epithelial expression in 17 patients with stage III-N2 non-small cell lung cancer (NSCLC) treated with definitive chemoradiotherapy. Results Id1 expression is observed in 82.4% of the tumors, whereas Id3 expression is present in 41.2% of the samples. Interestingly, Id1 and Id3 expression are mutually correlated (R = 0.579, p = 0.015). In a subgroup analysis of patients with the most locally advanced disease (T4N2 stage), co-expression of Id1 and Id3 showed to be related with a worse overall survival (45 vs 6 months, p = 0.002). A trend towards significance for a worse progression free survival (30 vs 1 months, p = 0.219) and a lower response rate to the treatment (RR = 50% vs 87.5%, p = 0.07) were also observed. Conclusions A correlation between Id1 and Id3 protein expression is observed. Id1 and Id3 co-expression seems associated with a poor clinical outcome in patients with locally advanced NSCLC treated with definitive chemoradiotherapy. PMID:23311395

  10. Substance use and misuse in persons with intellectual disabilities (ID): results of a survey in ID and addiction services in Flanders.

    PubMed

    To, Wing Ting; Neirynck, Soetkin; Vanderplasschen, Wouter; Vanheule, Stijn; Vandevelde, Stijn

    2014-01-01

    Little is known about the characteristics of substance users with intellectual disabilities (ID). Nevertheless, this group is assumed to be at greater risk of developing substance misuse problems. This study focuses on substance users and misusers with ID, and investigates whether the two groups differ significantly in terms of the nature and consequences of their substance (mis)use. Information regarding the characteristics of the substance (mis)users, the substances used, the negative consequences of substance (mis)use, and the service use was collected through a questionnaire forwarded to ID and addiction services in Flanders. Caregivers identified 104 substance users and misusers with ID. Overall, few differences were observed between users and misusers. This finding underscores that substance use in persons with ID can have important consequences. Substance misusers, however, were found to have more mood changes, more suicidal ideation/thoughts, and more negative long-term consequences on their health, daily activity, and relationships due to substance misuse. Substance use and misuse were associated with mental health problems and were suggested to be a risk factor for offending behavior. To provide appropriate support for this specific population, an individualized approach is suggested that supports better intersectoral collaboration between services.

  11. Unconventional myosin ID is expressed in myelinating oligodendrocytes.

    PubMed

    Yamazaki, Reiji; Ishibashi, Tomoko; Baba, Hiroko; Yamaguchi, Yoshihide

    2014-10-01

    Myelin is a dynamic multilamellar structure that ensheathes axons and is crucial for normal neuronal function. In the central nervous system (CNS), myelin is produced by oligodendrocytes that wrap many layers of plasma membrane around axons. The dynamic membrane trafficking system, which relies on motor proteins, is required for myelin formation and maintenance. Previously, we found that myosin ID (Myo1d), a class I myosin, is enriched in the rat CNS myelin fraction. Myo1d is an unconventional myosin and has been shown to be involved in membrane trafficking in the recycling pathway in an epithelial cell line. Western blotting revealed that Myo1d expression begins early in myelinogenesis and continues to increase into adulthood. The localization of Myo1d in CNS myelin has not been reported, and the function of Myo1d in vivo remains unknown. To demonstrate the expression of Myo1d in CNS myelin and to begin to explore the function of Myo1d in myelination, we produced a new antibody against Myo1d that has a high titer and specificity for rat Myo1d. By using this antibody, we demonstrated that Myo1d is expressed in rat CNS myelin and is especially abundant in abaxonal and adaxonal regions (the outer and inner cytoplasm-containing loops, respectively), but that expression is low in peripheral nervous system myelin. In culture, Myo1d was expressed in mature rat oligodendrocytes. Furthermore, an increase in expression of Myo1d during maturation of CNS white matter (cerebellum and corpus callosum) was demonstrated by histological analysis. These results suggest that Myo1d may be involved in the formation and/or maintenance of CNS myelin.

  12. The ID23-2 structural biology microfocus beamline at the ESRF

    PubMed Central

    Flot, David; Mairs, Trevor; Giraud, Thierry; Guijarro, Matias; Lesourd, Marc; Rey, Vicente; van Brussel, Denis; Morawe, Christian; Borel, Christine; Hignette, Olivier; Chavanne, Joel; Nurizzo, Didier; McSweeney, Sean; Mitchell, Edward

    2010-01-01

    The first phase of the ESRF beamline ID23 to be constructed was ID23-1, a tunable MAD-capable beamline which opened to users in early 2004. The second phase of the beamline to be constructed is ID23-2, a monochromatic microfocus beamline dedicated to macromolecular crystallography experiments. Beamline ID23-2 makes use of well characterized optical elements: a single-bounce silicon (111) monochromator and two mirrors in Kirkpatrick–Baez geometry to focus the X-ray beam. A major design goal of the ID23-2 beamline is to provide a reliable, easy-to-use and routine microfocus beam. ID23-2 started operation in November 2005, as the first beamline dedicated to microfocus macromolecular crystallography. The beamline has taken the standard automated ESRF macromolecular crystallography environment (both hardware and software), allowing users of ID23-2 to be rapidly familiar with the microfocus environment. This paper describes the beamline design, the special considerations taken into account given the microfocus beam, and summarizes the results of the first years of the beamline operation. PMID:20029119

  13. Bio-fortification and shelf-life extension of idli batter using curry leaves (Murraya koenigii).

    PubMed

    Chelliah, R; Ramakrishnan, S R; Premkumar, D; Antony, U

    2016-06-01

    Among several traditional foods of India, idli is one of the most popular and commonly consumed steamed products. A new method of adding Murraya koenigii (curry leaves) to idli batter as a vehicle for fortification and extension of shelf-life has been developed. Dried curry leaves powder was incorporated with other ingredients like rice and dehusked black gram in different proportions to optimize the most palatable formulation. Rate of fermentation and microbial changes in the batter; nutritional qualities, texture and sensory properties of the prepared product were assessed. Incorporation of curry leaves powder (5 %) in idli batter increased the shelf-life and also increased the flavour, texture and appearance of the idli. The calcium content of the prepared idli was 10 times more than that of the control idli, while dietary fiber content increased by 18.6 %. Anti-microbial activity of the curry leaves in idli batter extended the shelf-life from 2 to 5 days when stored at 30 °C.

  14. SpDamID: Marking DNA Bound by Protein Complexes Identifies Notch-Dimer Responsive Enhancers

    PubMed Central

    Hass, Matthew R.; Liow, Hien-haw; Chen, Xiaoting; Sharma, Ankur; Inoue, Yukiko U.; Inoue, Takayoshi; Reeb, Ashley; Martens, Andrew; Fulbright, Mary; Raju, Saravanan; Stevens, Michael; Boyle, Scott; Park, Joo-Seop; Weirauch, Matthew T.; Brent, Michael; Kopan, Raphael

    2015-01-01

    SUMMARY We developed Split DamID (SpDamID), a protein complementation version of DamID, to mark genomic DNA bound in vivo by interacting or juxtapositioned transcription factors. Inactive halves of DAM (DNA Adenine Methyltransferase) were fused to protein pairs to be queried Interaction or proximity enabled DAM reconstitution and methylation of adenine in GATC. Inducible SpDamID was used to analyze Notch-mediated transcriptional activation. We demonstrate that Notch complexes label RBP sites broadly across the genome, and show that a subset of these complexes that recruit MAML and p300 undergo changes in chromatin accessibility in response to Notch signaling. SpDamID differentiates between monomeric and dimeric binding thereby allowing for identification of half-site motifs used by Notch dimers. Motif enrichment of Notch enhancers coupled with SpDamID reveals co-targeting of regulatory sequences by Notch and Runx1. SpDamID represents a sensitive and powerful tool that enables dynamic analysis of combinatorial protein-DNA transactions at a genome-wide level. PMID:26257285

  15. Bio-fortification and shelf-life extension of idli batter using curry leaves (Murraya koenigii).

    PubMed

    Chelliah, R; Ramakrishnan, S R; Premkumar, D; Antony, U

    2016-06-01

    Among several traditional foods of India, idli is one of the most popular and commonly consumed steamed products. A new method of adding Murraya koenigii (curry leaves) to idli batter as a vehicle for fortification and extension of shelf-life has been developed. Dried curry leaves powder was incorporated with other ingredients like rice and dehusked black gram in different proportions to optimize the most palatable formulation. Rate of fermentation and microbial changes in the batter; nutritional qualities, texture and sensory properties of the prepared product were assessed. Incorporation of curry leaves powder (5 %) in idli batter increased the shelf-life and also increased the flavour, texture and appearance of the idli. The calcium content of the prepared idli was 10 times more than that of the control idli, while dietary fiber content increased by 18.6 %. Anti-microbial activity of the curry leaves in idli batter extended the shelf-life from 2 to 5 days when stored at 30 °C. PMID:27478242

  16. Id1 expression promotes T regulatory cell differentiation by facilitating TCR costimulation.

    PubMed

    Liu, Chen; Wang, Hong-Cheng; Yu, Sen; Jin, Rong; Tang, Hui; Liu, Yuan-Feng; Ge, Qing; Sun, Xiao-Hong; Zhang, Yu

    2014-07-15

    T regulatory (Treg) cells play crucial roles in the regulation of cellular immunity. The development of Treg cells depends on signals from TCRs and IL-2Rs and is influenced by a variety of transcription factors. The basic helix-loop-helix proteins are known to influence TCR signaling thresholds. Whether this property impacts Treg differentiation is not understood. In this study, we interrogated the role of basic helix-loop-helix proteins in the production of Treg cells using the CD4 promoter-driven Id1 transgene. We found that Treg cells continued to accumulate as Id1 transgenic mice aged, resulting in a significant increase in Treg cell counts in the thymus as well as in the periphery compared with wild-type controls. Data from mixed bone marrow assays suggest that Id1 acts intrinsically on developing Treg cells. We made a connection between Id1 expression and CD28 costimulatory signaling because Id1 transgene expression facilitated the formation of Treg precursors in CD28(-/-) mice and the in vitro differentiation of Treg cells on thymic dendritic cells despite the blockade of costimulation by anti-CD80/CD86. Id1 expression also allowed in vitro Treg differentiation without anti-CD28 costimulation, which was at least in part due to enhanced production of IL-2. Notably, with full strength of costimulatory signals, however, Id1 expression caused modest but significant suppression of Treg induction. Finally, we demonstrate that Id1 transgenic mice were less susceptible to the induction of experimental autoimmune encephalomyelitis, thus illustrating the impact of Id1-mediated augmentation of Treg cell levels on cellular immunity.

  17. The metalloid arsenite induces nuclear export of Id3 possibly via binding to the N-terminal cysteine residues

    SciTech Connect

    Kurooka, Hisanori; Sugai, Manabu; Mori, Kentaro; Yokota, Yoshifumi

    2013-04-19

    Highlights: •Sodium arsenite induces cytoplasmic accumulation of Id3. •Arsenite binds to closely spaced N-terminal cysteine residues of Id3. •N-terminal cysteines are essential for arsenite-induced nuclear export of Id3. •Nuclear export of Id3 counteracts its transcriptional repression activity. -- Abstract: Ids are versatile transcriptional repressors that regulate cell proliferation and differentiation, and appropriate subcellular localization of the Id proteins is important for their functions. We previously identified distinct functional nuclear export signals (NESs) in Id1 and Id2, but no active NES has been reported in Id3. In this study, we found that treatment with the stress-inducing metalloid arsenite led to the accumulation of GFP-tagged Id3 in the cytoplasm. Cytoplasmic accumulation was impaired by a mutation in the Id3 NES-like sequence resembling the Id1 NES, located at the end of the HLH domain. It was also blocked by co-treatment with the CRM1-specific nuclear export inhibitor leptomycin B (LMB), but not with the inhibitors for mitogen-activated protein kinases (MAPKs). Importantly, we showed that the closely spaced N-terminal cysteine residues of Id3 interacted with the arsenic derivative phenylarsine oxide (PAO) and were essential for the arsenite-induced cytoplasmic accumulation, suggesting that arsenite induces the CRM1-dependent nuclear export of Id3 via binding to the N-terminal cysteines. Finally, we demonstrated that Id3 significantly repressed arsenite-stimulated transcription of the immediate-early gene Egr-1 and that this repression activity was inversely correlated with the arsenite-induced nuclear export. Our results imply that Id3 may be involved in the biological action of arsenite.

  18. Id-1 gene and gene products as therapeutic targets for treatment of breast cancer and other types of carcinoma

    DOEpatents

    Desprez, Pierre-Yves; Campisi, Judith

    2014-08-19

    A method for treatment of breast cancer and other types of cancer. The method comprises targeting and modulating Id-1 gene expression, if any, for the Id-1 gene, or gene products in breast or other epithelial cancers in a patient by delivering products that modulate Id-1 gene expression. When expressed, Id-1 gene is a prognostic indicator that cancer cells are invasive and metastatic.

  19. Improvement and analysis of ID3 algorithm in decision-making tree

    NASA Astrophysics Data System (ADS)

    Xie, Xiao-Lan; Long, Zhen; Liao, Wen-Qi

    2015-12-01

    For the cooperative system under development, it needs to use the spatial analysis and relative technology concerning data mining in order to carry out the detection of the subject conflict and redundancy, while the ID3 algorithm is an important data mining. Due to the traditional ID3 algorithm in the decision-making tree towards the log part is rather complicated, this paper obtained a new computational formula of information gain through the optimization of algorithm of the log part. During the experiment contrast and theoretical analysis, it is found that IID3 (Improved ID3 Algorithm) algorithm owns higher calculation efficiency and accuracy and thus worth popularizing.

  20. Wnt/{beta}-catenin signaling changes C2C12 myoblast proliferation and differentiation by inducing Id3 expression

    SciTech Connect

    Zhang, Long; Shi, Songting; Zhang, Juan; Zhou, Fangfang; Dijke, Peter ten

    2012-03-02

    Highlights: Black-Right-Pointing-Pointer Expression of Id3 but not Id1 is induced by Wnt3a stimulation in C2C12 cells. Black-Right-Pointing-Pointer Wnt3a induces Id3 expression via canonical Wnt/{beta}-catenin pathway. Black-Right-Pointing-Pointer Wnt3a-induced Id3 expression does not depend on BMP signaling activation. Black-Right-Pointing-Pointer Induction of Id3 expression is critical determinant in Wnt3a-induced cell proliferation and differentiation. -- Abstract: Canonical Wnt signaling plays important roles in regulating cell proliferation and differentiation. In this study, we report that inhibitor of differentiation (Id)3 is a Wnt-inducible gene in mouse C2C12 myoblasts. Wnt3a induced Id3 expression in a {beta}-catenin-dependent manner. Bone morphogenetic protein (BMP) also potently induced Id3 expression. However, Wnt-induced Id3 expression occurred independent of the BMP/Smad pathway. Functional studies showed that Id3 depletion in C2C12 cells impaired Wnt3a-induced cell proliferation and alkaline phosphatase activity, an early marker of osteoblast cells. Id3 depletion elevated myogenin induction during myogenic differentiation and partially impaired Wnt3a suppressed myogenin expression in C2C12 cells. These results suggest that Id3 is an important Wnt/{beta}-catenin induced gene in myoblast cell fate determination.

  1. Family Impact in Intellectual Disability, Severe Mental Health Disorders and Mental Health Disorders in ID. A Comparison

    ERIC Educational Resources Information Center

    Martorell, Almudena; Gutierrez-Recacha, Pedro; Irazabal, Marcia; Marsa, Ferran; Garcia, Mercedes

    2011-01-01

    Family impact (or family burden) is a concept born in the field of mental health that has successfully been exported to the ambit of intellectual disability (ID). However, differences in family impact associated with severe mental health disorders (schizophrenia), to ID or to mental health problems in ID should be expected. Seventy-two adults with…

  2. 6 CFR 37.5 - Validity periods and deadlines for REAL ID driver's licenses and identification cards.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... SECURITY, OFFICE OF THE SECRETARY REAL ID DRIVER'S LICENSES AND IDENTIFICATION CARDS General § 37.5 Validity periods and deadlines for REAL ID driver's licenses and identification cards. (a) Driver's... individuals born after December 1, 1964, unless such license or card is a REAL ID-compliant driver's...

  3. 6 CFR 37.5 - Validity periods and deadlines for REAL ID driver's licenses and identification cards.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 6 Domestic Security 1 2011-01-01 2011-01-01 false Validity periods and deadlines for REAL ID... SECURITY, OFFICE OF THE SECRETARY REAL ID DRIVER'S LICENSES AND IDENTIFICATION CARDS General § 37.5 Validity periods and deadlines for REAL ID driver's licenses and identification cards. (a)...

  4. The Director's Link, 2003.

    ERIC Educational Resources Information Center

    Clark, Doug, Ed.

    This document consists of the 2003 issues of a quarterly newsletter for Illinois early childhood program directors, providing information on current issues in early childhood education. Regular features in each issue include: "The Director's Toolbox," delineating practical suggestions for directors related to program policies and staff issues;…

  5. Sexual abuse involving children with an intellectual disability (ID): a narrative review.

    PubMed

    Wissink, Inge B; van Vugt, Eveline; Moonen, Xavier; Stams, Geert-Jan J M; Hendriks, Jan

    2015-01-01

    The current paper provides a narrative review of the literature on sexual abuse, involving children with Intellectual Disability (ID). The thirteen articles that were found and met our criteria vary in their definitions of sexual abuse and in how ID was determined. Still, they do paint a general picture concerning (1) the extent of sexual abuse, (2) the nature of the sexual abuse, and (3) the institutional reactions following sexual abuse of children with ID. Our findings confirm the greater vulnerability of children with ID to become involved in sexual abuse both as a victim and as a perpetrator, and we discuss ways to help strengthening prevention and intervention methods. Nevertheless, more research is needed, as it is still a rather unexplored topic, which is striking in light of the high vulnerability of this group.

  6. Identification of an alternative transcript from the human iduronate-2-sulfatase (IDS) gene

    SciTech Connect

    Malmgren, H.; Carlberg, B.M.; Petterson, U.

    1995-09-01

    Iduronate-2-sulfatase (IDS) is involved in the degradation of heparan sulfate and dermatan sulfate in the lysosomes, and a deficiency in this enzyme results in Hunter syndrome. A 2.3-kb cDNA clone that contains the entire coding sequence of IDS has previously been reported. Here we describe the identification of a 1.4-kb transcript that may encode an IDS-like enzyme. The predicted protein is identical to the previously described enzyme, except for the absence of the 207-amino-acid COOH-terminal domain, which is replaced by 7 amino acids. Our data suggest that there might exist an additional form of the IDS enzyme in humans. The results from this study may have implications for the pathogenesis of the Hunter syndrome. 16 refs., 2 figs.

  7. Id2 IS REQUIRED FOR SPECIFICATION OF DOPAMINERGIC NEURONS DURING ADULT OLFACTORY NEUROGENESIS

    PubMed Central

    Havrda, Matthew C.; Harris, Brent T.; Mantani, Akio; Ward, Nora M.; Paolella, Brenton R.; Cuzon, Verginia C.; Yeh, Hermes H.; Israel, Mark A.

    2009-01-01

    Understanding the biology of adult neural stem cells has important implications for nervous system development and may contribute to our understanding of neurodegenerative disorders and their treatment. We have characterized the process of olfactory neurogenesis in adult mice lacking Inhibitor of DNA Binding 2 (Id2). We found a diminished olfactory bulb containing reduced numbers of granular and periglomerular neurons with a distinct paucity of dopaminergic periglomerular neurons. While no deficiency of the stem cell compartment was detectable, migrating neuroblasts in Id2−/− mutant mice prematurely undergo astroglial differentiation within a disorganized rostral migratory stream. Further, when evaluated in vitro loss of Id2 results in decreased proliferation of neural progenitors and decreased expression of the Hes1 and Mash1 transcription factors, known mediators of neuronal differentiation. These data support a novel role for sustained Id2 expression in migrating neural progenitors mediating olfactory dopaminergic neuronal differentiation in adult animals. PMID:19109490

  8. 78 FR 68467 - Notice of Public Meetings, Twin Falls District Resource Advisory Council, ID

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-11-14

    ... Resource Advisory Council (RAC) and subcommittee for the Greater sage-grouse will meet as indicated below... sage-grouse will meet at the Twin Falls District BLM Office, 2536 Kimberly Road, Twin Falls, ID...

  9. 77 FR 55813 - Transition of DOE-ID Public Reading Room

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-09-11

    ... Library at 1776 Science Center Drive, Idaho Falls, ID 83401, beginning September 1, 2012. Access to....ntis.gov . DATES: Effective September 1, 2012. ADDRESSES: INL Research Library, 1776 Science...

  10. Phosphatidylinositol-3 kinase-dependent translational regulation of Id1 involves the PPM1G phosphatase

    PubMed Central

    Xu, Kaiming; Wang, Lanfang; Feng, Wei; Feng, Yue; Shu, Hui-Kuo G.

    2016-01-01

    Id1 is a helix-loop-helix transcriptional modulator that increases the aggressiveness of malignant glial neoplasms. Since most glioblastomas (GBMs) show increased phosphatidylinositol-3 kinase (PI-3K) signaling, we sought to determine whether this pathway regulates Id1 expression. Higher basal Id1 expression correlates with dysregulated PI-3K signaling in multiple established GBM cell lines. Further characterization of PI-3K-dependent Id1 regulation reveals that chemical or genetic inhibition of PI-3K signaling reduces Id1 protein but not mRNA expression. Overall, PI-3K signaling appears to enhance Id1 translation with no significant effect on its stability. PI-3K signaling is known to regulate protein translation through mTORC1-dependent phosphorylation of 4E-BP1, which reduces its association with and inhibition of the translation initiation factor eIF4E. Interestingly, while inhibition of PI-3K and AKT lowers 4E-BP1 phosphorylation and expression of Id1 in all cases, inhibition of TORC1 with rapamycin does not consistently have a similar effect suggesting an alternative mechanism for PI-3K-dependent regulation of Id1 translation. We now identify a potential role for the serine-threonine phosphatase PPM1G in translational regulation of Id1 protein expression. PPM1G knockdown by siRNA increase both 4E-BP1 phosphorylation and Id1 expression and PPM1G and 4E-BP1 co-associates in GBM cells. Furthermore, PPM1G is a phosphoprotein and this phosphorylation appears to be regulated by PI-3K activity. Finally, PI-3K inhibition increases PPM1G activity when assessed by an in vitro phosphatase assay. Our findings provide the first evidence that the PI-3K/AKT signaling pathway modulates PPM1G activity resulting in a shift in the balance between hyper- and hypo-phosphorylated 4E-BP1 and translational regulation of Id1 expression. PMID:27065332

  11. Issues in energy finance

    NASA Astrophysics Data System (ADS)

    Khokher, Zeigham Islam

    As opposed to the well developed and understood equity markets, the energy markets are still in their infancy. The explosion of contracts, of both the primary and derivative types, are testament both to the existing size and the untapped growth potential of this exciting industry. However, because of its relative youth many basic issues in the energy markets remain unresolved. This thesis introduces some interesting questions and provides insights into these issues. Thematically, the chapters of this thesis are linked by an emphasis on valuation and risk management decisions. A contribution of this thesis is to show that subtle differences between the endogenous price process in our general equilibrium setting and the exogenous processes considered in earlier papers can generate significant differences in both financial and real option values. In addition to these valuation concerns there has been much debate about the corporate risk management function. Finance theory suggests that a value maximizing corporation should either be indifferent to hedging or, in the presence of certain imperfections, it should completely hedge all exposures. Both these extremes contradict empirical evidence. We show that existing corporate hedging behaviour is best explained in light of both physical market imperfections and directional predictions on future prices. While these speculative motives may arise from corporate hubris or genuine informational advantages, we argue that it would be difficult to implement private information in the absence of noise traders. Related to the risk management decision is the existence of futures risk premia. These premia have been thought to be cause by covariance with priced factors or due to the hedging demands of consumers and producers. This thesis argues that inventories serve as a signal of available quantity, which coupled with consumers fears regarding stockouts can induce a positive relationship between premia and inventories. In

  12. ETR BUILDING, TRA642, INTERIOR. BASEMENT. CUBICLE SHOWN IN ID33G101, ANOTHER ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    ETR BUILDING, TRA-642, INTERIOR. BASEMENT. CUBICLE SHOWN IN ID-33-G-101, ANOTHER VIEW. PERSONNEL DOORWAY INTO CHAMBER IDENTIFIES SODIUM HAZARD AND POSSIBILITY OF INERT GAS. LIQUID SODIUM COOLANT WAS USED IN A SPECIAL ETR LOOP ADAPTED FOR IT IN 1972. INL NEGATIVE NO. HD24-3-2. Mike Crane, Photographer, 11/2000 - Idaho National Engineering Laboratory, Test Reactor Area, Materials & Engineering Test Reactors, Scoville, Butte County, ID

  13. Gall-ID: tools for genotyping gall-causing phytopathogenic bacteria.

    PubMed

    Davis Ii, Edward W; Weisberg, Alexandra J; Tabima, Javier F; Grunwald, Niklaus J; Chang, Jeff H

    2016-01-01

    Understanding the population structure and genetic diversity of plant pathogens, as well as the effect of agricultural practices on pathogen evolution, is important for disease management. Developments in molecular methods have contributed to increase the resolution for accurate pathogen identification, but those based on analysis of DNA sequences can be less straightforward to use. To address this, we developed Gall-ID, a web-based platform that uses DNA sequence information from 16S rDNA, multilocus sequence analysis and whole genome sequences to group disease-associated bacteria to their taxonomic units. Gall-ID was developed with a particular focus on gall-forming bacteria belonging to Agrobacterium, Pseudomonas savastanoi, Pantoea agglomerans, and Rhodococcus. Members of these groups of bacteria cause growth deformation of plants, and some are capable of infecting many species of field, orchard, and nursery crops. Gall-ID also enables the use of high-throughput sequencing reads to search for evidence for homologs of characterized virulence genes, and provides downloadable software pipelines for automating multilocus sequence analysis, analyzing genome sequences for average nucleotide identity, and constructing core genome phylogenies. Lastly, additional databases were included in Gall-ID to help determine the identity of other plant pathogenic bacteria that may be in microbial communities associated with galls or causative agents in other diseased tissues of plants. The URL for Gall-ID is http://gall-id.cgrb.oregonstate.edu/. PMID:27547538

  14. Gall-ID: tools for genotyping gall-causing phytopathogenic bacteria

    PubMed Central

    Tabima, Javier F.; Grunwald, Niklaus J.

    2016-01-01

    Understanding the population structure and genetic diversity of plant pathogens, as well as the effect of agricultural practices on pathogen evolution, is important for disease management. Developments in molecular methods have contributed to increase the resolution for accurate pathogen identification, but those based on analysis of DNA sequences can be less straightforward to use. To address this, we developed Gall-ID, a web-based platform that uses DNA sequence information from 16S rDNA, multilocus sequence analysis and whole genome sequences to group disease-associated bacteria to their taxonomic units. Gall-ID was developed with a particular focus on gall-forming bacteria belonging to Agrobacterium, Pseudomonas savastanoi, Pantoea agglomerans, and Rhodococcus. Members of these groups of bacteria cause growth deformation of plants, and some are capable of infecting many species of field, orchard, and nursery crops. Gall-ID also enables the use of high-throughput sequencing reads to search for evidence for homologs of characterized virulence genes, and provides downloadable software pipelines for automating multilocus sequence analysis, analyzing genome sequences for average nucleotide identity, and constructing core genome phylogenies. Lastly, additional databases were included in Gall-ID to help determine the identity of other plant pathogenic bacteria that may be in microbial communities associated with galls or causative agents in other diseased tissues of plants. The URL for Gall-ID is http://gall-id.cgrb.oregonstate.edu/. PMID:27547538

  15. EcID. A database for the inference of functional interactions in E. coli.

    PubMed

    Andres Leon, Eduardo; Ezkurdia, Iakes; García, Beatriz; Valencia, Alfonso; Juan, David

    2009-01-01

    The EcID database (Escherichia coli Interaction Database) provides a framework for the integration of information on functional interactions extracted from the following sources: EcoCyc (metabolic pathways, protein complexes and regulatory information), KEGG (metabolic pathways), MINT and IntAct (protein interactions). It also includes information on protein complexes from the two E. coli high-throughput pull-down experiments and potential interactions extracted from the literature using the web services associated to the iHOP text-mining system. Additionally, EcID incorporates results of various prediction methods, including two protein interaction prediction methods based on genomic information (Phylogenetic Profiles and Gene Neighbourhoods) and three methods based on the analysis of co-evolution (Mirror Tree, In Silico 2 Hybrid and Context Mirror). EcID associates to each prediction a specifically developed confidence score. The two main features that make EcID different from other systems are the combination of co-evolution-based predictions with the experimental data, and the introduction of E. coli-specific information, such as gene regulation information from EcoCyc. The possibilities offered by the combination of the EcID database information are illustrated with a prediction of potential functions for a group of poorly characterized genes related to yeaG. EcID is available online at http://ecid.bioinfo.cnio.es.

  16. Gall-ID: tools for genotyping gall-causing phytopathogenic bacteria.

    PubMed

    Davis Ii, Edward W; Weisberg, Alexandra J; Tabima, Javier F; Grunwald, Niklaus J; Chang, Jeff H

    2016-01-01

    Understanding the population structure and genetic diversity of plant pathogens, as well as the effect of agricultural practices on pathogen evolution, is important for disease management. Developments in molecular methods have contributed to increase the resolution for accurate pathogen identification, but those based on analysis of DNA sequences can be less straightforward to use. To address this, we developed Gall-ID, a web-based platform that uses DNA sequence information from 16S rDNA, multilocus sequence analysis and whole genome sequences to group disease-associated bacteria to their taxonomic units. Gall-ID was developed with a particular focus on gall-forming bacteria belonging to Agrobacterium, Pseudomonas savastanoi, Pantoea agglomerans, and Rhodococcus. Members of these groups of bacteria cause growth deformation of plants, and some are capable of infecting many species of field, orchard, and nursery crops. Gall-ID also enables the use of high-throughput sequencing reads to search for evidence for homologs of characterized virulence genes, and provides downloadable software pipelines for automating multilocus sequence analysis, analyzing genome sequences for average nucleotide identity, and constructing core genome phylogenies. Lastly, additional databases were included in Gall-ID to help determine the identity of other plant pathogenic bacteria that may be in microbial communities associated with galls or causative agents in other diseased tissues of plants. The URL for Gall-ID is http://gall-id.cgrb.oregonstate.edu/.

  17. Exploring the Issue of Employment for Adults with an Intellectual Disability in Ireland

    ERIC Educational Resources Information Center

    McGlinchey, Eimear; McCallion, Philip; Burke, Eilish; Carroll, Rachel; McCarron, Mary

    2013-01-01

    Background: The benefits of being in employment are well documented; however, underemployment is a critical issue for people with intellectual disability (ID). Some individuals perceive themselves as being in employment when in fact they are attending a non-work site such as a day service. The impact that this perception of employment has on other…

  18. miR-342 Regulates BRCA1 Expression through Modulation of ID4 in Breast Cancer

    PubMed Central

    Crippa, Elisabetta; Lusa, Lara; De Cecco, Loris; Marchesi, Edoardo; Calin, George Adrian; Radice, Paolo; Manoukian, Siranoush; Peissel, Bernard; Daidone, Maria Grazia

    2014-01-01

    A miRNAs profiling on a group of familial and sporadic breast cancers showed that miRNA-342 was significantly associated with estrogen receptor (ER) levels. To investigate at functional level the role of miR-342 in the pathogenesis of breast cancer, we focused our attention on its “in silico” predicted putative target gene ID4, a transcription factor of the helix-loop-helix protein family whose expression is inversely correlated with that of ER. ID4 is expressed in breast cancer and can negatively regulate BRCA1 expression. Our results showed an inverse correlation between ID4 and miR-342 as well as between ID4 and BRCA1 expression. We functionally validated the interaction between ID4 and miR-342 in a reporter Luciferase system. Based on these findings, we hypothesized that regulation of ID4 mediated by miR-342 could be involved in the pathogenesis of breast cancer by downregulating BRCA1 expression. We functionally demonstrated the interactions between miR-342, ID4 and BRCA1 in a model provided by ER-negative MDA-MB-231 breast cancer cell line that presented high levels of ID4. Overexpression of miR-342 in these cells reduced ID4 and increased BRCA1 expression, supporting a possible role of this mechanism in breast cancer. In the ER-positive MCF7 and in the BRCA1-mutant HCC1937 cell lines miR-342 over-expression only reduced ID4. In the cohort of patients we studied, a correlation between miR-342 and BRCA1 expression was found in the ER-negative cases. As ER-negative cases were mainly BRCA1-mutant, we speculate that the mechanism we demonstrated could be involved in the decreased expression of BRCA1 frequently observed in non BRCA1-mutant breast cancers and could be implicated as a causal factor in part of the familial cases grouped in the heterogeneous class of non BRCA1 or BRCA2-mutant cases (BRCAx). To validate this hypothesis, the study should be extended to a larger cohort of ER-negative cases, including those belonging to the BRCAx class. PMID:24475217

  19. Challenges with using primer IDs to improve accuracy of next generation sequencing.

    PubMed

    Brodin, Johanna; Hedskog, Charlotte; Heddini, Alexander; Benard, Emmanuel; Neher, Richard A; Mild, Mattias; Albert, Jan

    2015-01-01

    Next generation sequencing technologies, like ultra-deep pyrosequencing (UDPS), allows detailed investigation of complex populations, like RNA viruses, but its utility is limited by errors introduced during sample preparation and sequencing. By tagging each individual cDNA molecule with barcodes, referred to as Primer IDs, before PCR and sequencing these errors could theoretically be removed. Here we evaluated the Primer ID methodology on 257,846 UDPS reads generated from a HIV-1 SG3Δenv plasmid clone and plasma samples from three HIV-infected patients. The Primer ID consisted of 11 randomized nucleotides, 4,194,304 combinations, in the primer for cDNA synthesis that introduced a unique sequence tag into each cDNA molecule. Consensus template sequences were constructed for reads with Primer IDs that were observed three or more times. Despite high numbers of input template molecules, the number of consensus template sequences was low. With 10,000 input molecules for the clone as few as 97 consensus template sequences were obtained due to highly skewed frequency of resampling. Furthermore, the number of sequenced templates was overestimated due to PCR errors in the Primer IDs. Finally, some consensus template sequences were erroneous due to hotspots for UDPS errors. The Primer ID methodology has the potential to provide highly accurate deep sequencing. However, it is important to be aware that there are remaining challenges with the methodology. In particular it is important to find ways to obtain a more even frequency of resampling of template molecules as well as to identify and remove artefactual consensus template sequences that have been generated by PCR errors in the Primer IDs.

  20. PTK7 regulates Id1 expression in CD44-high glioma cells

    PubMed Central

    Liu, Qing; Zhang, Chi; Yuan, Jian; Fu, Jun; Wu, Minghua; Su, Jun; Wang, Xiangyu; Yuan, Xianrui; Jiang, Weixi

    2015-01-01

    Background CD44 is a molecular marker associated with molecular subtype and treatment resistance in glioma. More effective therapies will result from approaches aimed at targeting the CD44-high gliomas. Methods Protein tyrosine kinase 7 (PTK7) mRNA expression was analyzed based on The Cancer Genome Atlas glioblastoma dataset. PTK7 expression was depleted through lentivirus-mediated short hairpin RNA knockdown. Terminal deoxynucleotidyl transferase dUTP nick-end labeling was used to evaluate cell apoptosis following PTK7 knockdown. Gene expression analysis was performed on Affymetrix microarray. A nude mice orthotopic tumor model was used to evaluate the in vivo effect of PTK7 depletion. Results PTK7 is highly expressed in CD44-high glioblastoma and predicts unfavorable prognosis. PTK7 knockdown attenuated cell proliferation, impaired tumorigenic potential, and induced apoptosis in CD44-high glioma cell lines. Gene expression analysis identified inhibitor of DNA Binding 1 (Id1) gene as a potential downstream effector for PTK7. Overexpression of Id1 mostly restored the cell proliferation and colony formation attenuated by PTK7 depletion. PTK7 enhanced anchorage-independent growth in normal human astrocytes, which was attenuated by Id1 knockdown. Furthermore, PTK7 regulated Id1 expression through modulating TGF-β/Smad signaling, while pharmacological inhibition on TGF-β/Smad signaling or PTK7/Id1 depletion attenuated TGF-β–stimulated cell proliferation. PTK7 depletion consistently reduced Id1 expression, suppressed tumor growth, and induced apoptosis in a murine orthotopic tumor model, which could be translated into prolonged survival in tumor-bearing mice. Conclusions PTK7 regulates Id1 expression in CD44-high glioma cell lines. Targeting PTK7 could be an effective strategy for treating glioma with high CD44 expression. PMID:25204555

  1. Current IT Issues, 2004

    ERIC Educational Resources Information Center

    Spicer, Donald Z.; Deblois, Peter B.

    2004-01-01

    This article features the EDUCAUSE Current Issues Survey. Administered by the EDUCAUSE Current Issues Committee, whose members review and recommend the set of issues to be presented each year, the survey identifies the issues that leaders in higher education information technology see as their most critical IT challenges. The Top-Ten current IT…

  2. Inhibitor of DNA binding 1 (Id1) induces differentiation and proliferation of mouse embryonic carcinoma P19CL6 cells

    SciTech Connect

    Meng, Qingzhen; Jia, Zhuqing; Wang, Weiping; Li, Binhong; Ma, Kangtao; Zhou, Chunyan

    2011-08-26

    Highlights: {yields} Id1 was upregulated during the cardiac differentiation process of P19CL6 cells. {yields} Id1 upregulated expression of cardiac specific genes Gata4, {alpha}-MHC and ISL1. {yields} Id1 promoted proliferation of P19CL6 cells. {yields} Overexpression of Id1 increased activity of TOP flash. {yields} Wnt3a or LiCl treatment promoted Id1 expression in P19CL6 cells. -- Abstract: The inhibitor of DNA binding (Id) family of genes encodes negative regulators of basic helix-loop-helix transcription factors and has been implicated in such diverse cellular processes as differentiation, proliferation, apoptosis and migration. Id knockout mouse embryos display multiple cardiac defects but the specific role of Id1 in cardiac differentiation is unclear. In the present study, we investigated the function of Id1 in DMSO-induced P19CL6 cells, a widely-accepted cell model of cardiac differentiation. We found that Id1 was upregulated during the cardiac differentiation of P19CL6 cells. The expression of cardiac specific marker genes, Gata4, {alpha}-MHC and ISL1, was upregulated in P19CL6 cells stably transfected with Id1 (P19CL6-Id1) during cardiac differentiation. The overexpression of Id1 reduced the number of cells in G1 phase and increased the cell population in G2, M and S phases, while knockdown of Id1 increased the number of cells in G1 phase from 48.6 {+-} 2.51% to 62.2 {+-} 1.52% at day 0 of cardiac induction, and from 52.5 {+-} 3.41% to 63.7 {+-} 1.02% at day 3 after cardiac induction, indicating that Id1 promoted proliferation of P19CL6 cells. Luciferase assays showed that the activity of TOP flash was higher in P19CL6-Id1 cells than wildtype P19CL6 cells, while Id1 expression was also upregulated in P19CL6 cells treated with Wnt3a or LiCl. This indicates that there may be positive feedback between Id1 and Wnt signaling which plays an important role in cardiac differentiation.

  3. The Director's Link, 2002.

    ERIC Educational Resources Information Center

    Clark, Doug, Ed.

    2002-01-01

    This document consists of the four 2002 issues of a quarterly newsletter for Illinois early childhood program directors, providing information on current issues in early childhood education. Regular features in each issue include: "The Director's Toolbox," delineating practical suggestions for directors related to program policies and staff…

  4. Id-1 promotes osteosarcoma cell growth and inhibits cell apoptosis via PI3K/AKT signaling pathway.

    PubMed

    Hao, Liang; Liao, Qi; Tang, Qiang; Deng, Huan; Chen, Lu

    2016-02-12

    Accumulating evidence reveals that Id-1 is upregulated and functions as a potential tumor promoter in several human cancer types. However, the role of Id-1 in osteosarcoma (OS) is unknown. In present study, we found that Id-1 expression was elevated in OS tissues than adjacent normal bone tissues. More importantly, we demonstrated that overexpression of Id-1 is significantly correlated with tumor progression and poor survival in OS patients. Furthermore, increased expression of Id-1 was observed in OS cell lines and ectopic expression of Id-1 significantly enhanced in vitro cell proliferation and promoted in vivo tumor growth, whereas knockdown of Id-1 suppressed OS cells growth. Moreover, our experimental data revealed that Id-1 promotes cell proliferation by facilitating cell cycle progression and inhibits cell apoptosis. Mechanistically, the effects of Id-1 in OS cells is at least partly through activation of PI3K/Akt signaling pathway. Therefore, we identified a tumorigenic role of Id-1 in OS and suggested a potential therapeutic target for OS patients.

  5. Dynamic programming for single nucleotide polymorphism ID identification in systematic association studies.

    PubMed

    Yang, Cheng-Hong; Chuang, Li-Yeh; Cheng, Yu-Huei; Wen, Cheng-Hao; Chang, Hsueh-Wei

    2009-04-01

    Single nucleotide polymorphisms (SNPs) play an important role in personalized medicine. However, the SNP data reported in many association studies provide only the SNP nucleotide/amino acid position, without providing the SNP ID recorded in National Center for Biotechnology Information databases. A tool with the ability to provide SNP ID identification, with a user-friendly interface, is needed. In this paper, a dynamic programming algorithm was used to compare homologs when the processed input sequence is aligned with the SNP FASTA database. Our novel system provides a web-based tool that uses the National Center for Biotechnology Information dbSNP database, which provides SNP sequence identification and SNP FASTA formats. Freely selectable sequence formats for alignment can be used, including general sequence formats (ACGT, [dNTP1/dNTP2] or IUPAC formats) and orientation with bidirectional sequence matching. In contrast to the National Center for Biotechnology Information SNP-BLAST, the proposed system always provides the correct targeted SNP ID (SNP hit), as well as nearby SNPs (flanking hits), arranged in their chromosomal order and contig positions. The system also solves problems inherent in SNP-BLAST, which cannot always provide the correct SNP ID for a given input sequence. Therefore, this system constitutes a novel application which uses dynamic programming to identify SNP IDs from the literature and keyed-in sequences for systematic association studies. It is freely available at http://bio.kuas.edu.tw/SNPosition/.

  6. A Comprehensive Analysis of Primer IDs to Study Heterogeneous HIV-1 Populations.

    PubMed

    Seifert, David; Di Giallonardo, Francesca; Töpfer, Armin; Singer, Jochen; Schmutz, Stefan; Günthard, Huldrych F; Beerenwinkel, Niko; Metzner, Karin J

    2016-01-16

    Determining the composition of viral populations is becoming increasingly important in the field of medical virology. While recently developed computational tools for viral haplotype analysis allow for correcting sequencing errors, they do not always allow for the removal of errors occurring in the upstream experimental protocol, such as PCR errors. Primer IDs (pIDs) are one method to address this problem by harnessing redundant template resampling for error correction. By using a reference mixture of five HIV-1 strains, we show how pIDs can be useful for estimating key experimental parameters, such as the substitution rate of the PCR process and the reverse transcription (RT) error rate. In addition, we introduce a hidden Markov model for determining the recombination rate of the RT PCR process. We found no strong sequence-specific bias in pID abundances (the same RT efficiencies as compared to commonly used short, specific RT primers) and no effects of pIDs on the estimated distribution of the references viruses.

  7. Usefulness of Candida ID2 agar for the presumptive identification of Candida dubliniensis.

    PubMed

    Eraso, Elena; Sahand, Ismail H; Villar-Vidal, María; Marcos, Cristina; Dolores Moragues, María; Madariaga, Lucila; Pontón, José; Quindós, Guillermo

    2006-11-01

    CHROMagar Candida and Candida ID2 are widely used for the isolation and presumptive identification of Candida spp. based on the color of the colonies on these two media. We have studied the usefulness of these chromogenic media for differentiating Candida dubliniensis from Candida albicans isolates. One hundred isolates of C. dubliniensis and 100 C. albicans isolates were tested on Candida ID2, CHROMagar Candida (CHROMagar), and CHROMagar Candida reformulated by BBL. CHROMagar Candida and CHROMagar Candida BBL did not allow a clear differentiation of the two species based upon the shade of the green color of C. dubliniensis colonies. However, on Candida ID2, all C. dubliniensis isolates produced turquoise blue colonies whereas 91% of C. albicans colonies were cobalt blue. The sensitivity and the specificity for differentiating between C. dubliniensis fromC. albicans on Candida ID2 were 100% and 91%, respectively; whereas on CHROMagar Candida these values were 63% and 89% and on CHROMagar Candida BBL they were 18% and 98%. Candida ID2 agar provides a simple and accurate laboratory approach for the identification and differentiation of C. dubliniensis on the basis of the colony color.

  8. Identifying Protein-Protein Associations at the Nuclear Envelope with BioID.

    PubMed

    Kim, Dae In; Jensen, Samuel C; Roux, Kyle J

    2016-01-01

    The nuclear envelope (NE) is a critical cellular structure whose constituents and roles in a myriad of cellular processes seem ever expanding. To determine the underlying mechanisms by which the NE constituents participate in various cellular events, it is necessary to understand the nature of their protein-protein associations. BioID (proximity-dependent biotin identification) is a recently established method to generate a history of protein-protein associations as they occur over time in living cells. BioID is based on fusion of a bait protein to a promiscuous biotin ligase. Expression of the BioID fusion protein in a relevant cellular environment enables biotinylation of vicinal and interacting proteins of the bait protein, permitting isolation and identification by conventional biotin-affinity capture and mass-spec analysis. In this way, BioID provides unique capabilities to identify protein-protein associations at the NE. In this chapter we provide a detailed protocol for the application of BioID to the study of NE proteins.

  9. Meet the neighbors: Mapping local protein interactomes by proximity‐dependent labeling with BioID

    PubMed Central

    Varnaitė, Renata

    2016-01-01

    Proximity‐dependent biotin identification (BioID) is a recently developed method that allows the identification of proteins in the close vicinity of a protein of interest in living cells. BioID relies on fusion of the protein of interest with a mutant form of the biotin ligase enzyme BirA (BirA*) that is capable of promiscuously biotinylating proximal proteins irrespective of whether these interact directly or indirectly with the fusion protein or are merely located in the same subcellular neighborhood. The covalent addition of biotin allows the labeled proteins to be purified from cell extracts on the basis of their affinity for streptavidin and identified by mass spectrometry. To date, BioID has been successfully applied to study a variety of proteins and processes in mammalian cells and unicellular eukaryotes and has been shown to be particularly suited to the study of insoluble or inaccessible cellular structures and for detecting weak or transient protein associations. Here, we provide an introduction to BioID, together with a detailed summary of where and how the method has been applied to date, and briefly discuss technical aspects involved in the planning and execution of a BioID study. PMID:27329485

  10. The use of the Kitt Peak IDS in determining galaxy redshifts - Results for five clusters

    NASA Astrophysics Data System (ADS)

    Hintzen, P.

    1980-06-01

    The redshifts of 71 galaxies in five clusters determined using the Kitt Peak Image Dissector Scanner (IDS) are presented and the accuracy of velocities determined with the IDS system is discussed. Data were obtained from observations of absorption or emission lines in the range 3600-5400 A using the IDS on a 2.1-m telescope and effective apertures of 3.8 x 10.3 arcsec from galaxies belonging to the AWM 7 = NGC 1129, A 1213, A 1795, A 2589 and A 2666 clusters. Wavelength errors due to mechanical flexure, off-center guiding and spectrum noise are estimated to provide an uncertainty of + or - 1.21 A or 92 km/sec in measurements of a given spectrum, and comparison of the velocities determined by the Kitt Peak IDS with those determined by the Lick IDS supports the error value. Implications of the mean heliocentric redshifts and relativistically correct velocity dispersions obtained for the structure and membership of the AWM 7 cluster are discussed.

  11. Attitudes of Israeli Teachers and Paraprofessionals towards the New Definition of ID and Their Willingness to Cope with Special Education Law Changes

    ERIC Educational Resources Information Center

    Lifshitz, Hefziba; Nissim, Shoshanna; Cohen, Sara

    2008-01-01

    Attitudes of teachers (N = 96) and paraprofessionals (N = 48) working with students with intellectual disability (ID) towards the new definition of ID (Luckasson et al., 1992, 2002), was examined. The main innovation of the new definition of ID is the cancellation of the four ID levels, suggesting an optimistic view, especially towards individuals…

  12. Synthesising Topological Links

    PubMed Central

    Baas, Nils A.; Seeman, Nadrian C.; Stacey, Andrew

    2014-01-01

    We discuss the chemical synthesis of topological links, in particular higher order links which have the Brunnian property (namely that removal of any one component unlinks the entire system). Furthermore, we suggest how to obtain both two dimensional and three dimensional objects (surfaces and solids, respectively) which also have this Brunnian property. PMID:25678732

  13. A West African Link

    ERIC Educational Resources Information Center

    Cook, Angela; Davies, Penny

    2003-01-01

    The authors visited Ghana in West Africa to strengthen a link established the previous year as part of Channel 4's "On the Line" project. The initial link established in 1999/2000 was between an all-age special school in Enfield and a similar school in Accra. Over the course of that year further partnerships were created between five UK schools…

  14. Web Usability Test Findings and Analysis Issues.

    ERIC Educational Resources Information Center

    An, Jae Soon; Park, Su-Hong; Baek, Eun-Ok

    This paper discusses findings and data analysis issues that resulted from a case usability test. The case usability test was conducted as part of a redesign project that aimed to update the existing home page of Indiana University Bloomington. The home page contained links to the Web sites that various institutions in the university had already…

  15. Educators and the Racial Issue in Education.

    ERIC Educational Resources Information Center

    Solomon, Benjamin

    1968-01-01

    This document points out the link between racism and education as interrelated social institutions, analyzes educators' positions on the issue, and offers a concept of educational integration. Within a racist society the schools and its teachers will perforce reflect the dominant pattern in all the components of education. A racist attitude among…

  16. E Proteins and ID Proteins: Helix-Loop-Helix Partners in Development and Disease.

    PubMed

    Wang, Lan-Hsin; Baker, Nicholas E

    2015-11-01

    The basic Helix-Loop-Helix (bHLH) proteins represent a well-known class of transcriptional regulators. Many bHLH proteins act as heterodimers with members of a class of ubiquitous partners, the E proteins. A widely expressed class of inhibitory heterodimer partners-the Inhibitor of DNA-binding (ID) proteins-also exists. Genetic and molecular analyses in humans and in knockout mice implicate E proteins and ID proteins in a wide variety of diseases, belying the notion that they are non-specific partner proteins. Here, we explore relationships of E proteins and ID proteins to a variety of disease processes and highlight gaps in knowledge of disease mechanisms.

  17. Comments on an ID-Based Authenticated Group Key Agreement Protocol with Withstanding Insider Attacks

    NASA Astrophysics Data System (ADS)

    Wu, Tsu-Yang; Tseng, Yuh-Min

    In PKC 2004, Choi et al. proposed an ID-based authenticated group key agreement (AGKA) protocol using bilinear pairings. Unfortunately, their protocol suffered from an impersonation attack and an insider colluding attack. In 2008, Choi et al. presented an improvement to resist insider attacks. In their modified protocol, they used an ID-based signature scheme on transcripts for binding them in a session to prevent replay of transcripts. In particular, they smartly used the batch verification technique to reduce the computational cost. In this paper, we first show that Choi et al.'s modified AGKA protocol still suffers from an insider colluding attack. Then, we prove that the batch verification of the adopted ID-based signature scheme in their modified protocol suffers from a forgery attack.

  18. Eosinophilic cellulitis and panniculitis with generalized vesicular pustular id reaction after a molten aluminum burn.

    PubMed

    Chao, Sheau-Chiou; Lee, Yi-Pei; Lee, Julia Yu-Yun

    2010-01-01

    Hypersensitivity reactions to aluminum are rare. Sensitization has been reported during the continuous application of aluminum-containing antiperspirants or after the injection of aluminum-adsorbed vaccines and allergen extracts containing aluminum. We report a rare case of a nonhealing ulcer at the site of an accidental molten aluminum burn that showed changes of eosinophilic cellulitis and panniculitis histologically. This process was followed by a generalized vesicular and pustular id reaction. The reactions resolved after 1 month of systemic corticosteroid therapy. Two months later, there was a relapse with bullae over the burn scar, accompanied by a pruritic oozing rash on the ears and face; the lesions were controlled again with systemic corticosteroid treatment. The clinicopathologic features suggest that the eosinophilic cellulitis with panniculitis and id reaction in the present case represents an unusual allergic reaction to aluminum. Aluminum allergy may be considered as a possible underlying process in the setting of eosinophilic cellulitis with panniculitis and id reaction.

  19. You can be a wizard of IDS. Is your practice ready to form an integrated delivery system?

    PubMed

    Fabrizio, Nick A

    2007-09-01

    To combat declining reimbursement and strengthen their positions in a highly competitive provider environment, leaders of large medical groups may consider forming an integrate delivery system (IDS) to gain a business advantage. Managing the array of organizational, operational, financial and human relationships affected by an IDS requires careful planning, good timing and a bit of luck. This article, the first of a two-part series, examines what questions physician practice leaders should ask--and answer--before formiing an IDS.

  20. ID-Based Blind Signature and Proxy Blind Signature without Trusted PKG

    NASA Astrophysics Data System (ADS)

    Yu, Yihua; Zheng, Shihui; Yang, Yixian

    Private key escrow is an inherent disadvantage for ID-based cryptosystem, i.e., the PKG knows each signer's private key and can forge the signature of any signer. Blind signature plays a central role in electronic cash system. Private key escrow is more severe in electronic cash system since money is directly involved. To avoid the key escrow problem, we propose an ID-based blind signature and proxy blind signature without trusted PKG. If the dishonest PKG impersonates an honest signer to sign a document, the signer can provide a proof to convince that the PKG is dishonest.

  1. Evaluation of the Mast ID and API 50CH systems for identification of Listeria spp.

    PubMed Central

    Kerr, K G; Rotowa, N A; Hawkey, P M; Lacey, R W

    1990-01-01

    A multipoint inoculation technique (Mast ID) for the identification and species determination of Listeria monocytogenes (sensu strictu) and six other species of the genus Listeria was evaluated. This was compared with the commercially available API 50CH system. Both methods successfully identified all 123 strains tested. The Mast ID system is inexpensive and utilizing a multipoint inoculation technique permits the screening of up to 21 isolates per 9-cm petri dish. The API 50CH system was more expensive and time consuming and is therefore suitable only for the examination of smaller numbers of strains. PMID:2180369

  2. Space Station Engineering Design Issues

    NASA Technical Reports Server (NTRS)

    Mcruer, Duane T.; Boehm, Barry W.; Debra, Daniel B.; Green, C. Cordell; Henry, Richard C.; Maycock, Paul D.; Mcelroy, John H.; Pierce, Chester M.; Stafford, Thomas P.; Young, Laurence R.

    1989-01-01

    Space Station Freedom topics addressed include: general design issues; issues related to utilization and operations; issues related to systems requirements and design; and management issues relevant to design.

  3. Issues in Peer Review.

    ERIC Educational Resources Information Center

    Sawamura, Mark H.

    2001-01-01

    Based on concerns raised at a workshop at the Southern California College of Optometry, addresses critical issues in the process of peer review of faculty teaching and possible alternatives to these issues as applied to an optometric institution. (EV)

  4. What! Teach Issues!?

    ERIC Educational Resources Information Center

    Rapoport, Faye

    1986-01-01

    Discusses how to incorporate the teaching of issues as well as natural history into environmental education to build environmentally aware citizens. Describes several issues-oriented curriculums which all use direct student participation in environmental conservation. (NEC)

  5. Medical Issues in Adoption

    MedlinePlus

    ... to Know About Zika & Pregnancy Medical Issues in Adoption KidsHealth > For Parents > Medical Issues in Adoption Print ... or emotional abuse of the child continue Agency Adoptions If you adopt through an agency, you might ...

  6. Emotional and Social Issues

    MedlinePlus

    ... Epinephrine Emotional & Social Issues Find a Support Group Bullying Prevention Spread the Word True Stories Stay Informed ... Epinephrine Emotional & Social Issues Find a Support Group Bullying Prevention Spread the Word True Stories Stay Informed ...

  7. Medical Issues: Equipment

    MedlinePlus

    ... Pool Living With SMA Medical Issues Palliative Breathing Orthopedics Nutrition Equipment Daily Life At School At Home ... Diagnosed Living With SMA Medical Issues Palliative Breathing Orthopedics Nutrition Equipment Daily Life Grief & Loss Community & Local ...

  8. Differential expression of id genes and their potential regulator znf238 in zebrafish adult neural progenitor cells and neurons suggests distinct functions in adult neurogenesis.

    PubMed

    Diotel, Nicolas; Beil, Tanja; Strähle, Uwe; Rastegar, Sepand

    2015-01-01

    Teleost fish display a remarkable ability to generate new neurons and to repair brain lesions during adulthood. They are, therefore, a very popular model to investigate the molecular mechanisms of constitutive and induced neurogenesis in adult vertebrates. In this study, we investigated the expression patterns of inhibitor of DNA binding (id) genes and of their potential transcriptional repressor, znf238, in the whole brain of adult zebrafish. We show that while id1 is exclusively expressed in ventricular cells in the whole brain, id2a, id3 and id4 genes are expressed in broader areas. Interestingly, znf238 was also detected in these regions, its expression overlapping with id2a, id3 and id4 expression. Further detailed characterization of the id-expressing cells demonstrated that (a) id1 is expressed in type 1 and type 2 neural progenitors as previously published, (b) id2a in type 1, 2 and 3 neural progenitors, (c) id3 in type 3 neural progenitors and (d) id4 in postmitotic neurons. Our data provide a detailed map of id and znf238 expression in the brain of adult zebrafish, supplying a framework for studies of id genes function during adult neurogenesis and brain regeneration in the zebrafish.

  9. Evidence on public policy: methodological issues, political issues and examples.

    PubMed

    Attanasio, Orazio P

    2014-03-01

    In this paper I discuss how evidence on public policy is generated and in particular the issue of evaluation of public policies. In economics, the issue of attribution and the identification of causal links has recently received considerable attention. Important methodological issues have been tackled and new techniques have been proposed and used. Randomized Control Trials have become some sort of gold standard. However, they are not exempt from problems and have important limitations: in some case they cannot be constructed and, more generally, problems of external validity and transferability of results can be important. The paper then moves on to discuss the political economy of policy evaluations for policy evaluations to have an impact for the conduct of actual policy, it is important that the demand for evaluation comes directly from the policy making process and is generated endogenously within it. In this sense it is important that the institutional design of policy making is such that policy making institutions are incentivized to use rigorous evaluation in the process of designing policies and allocating resources to alternative options. Economists are currently involved in the design and evaluation of many policies, including policies about health, nutrition and education. The role they can play in these fields is not completely obvious. The paper argues that their main contribution is in the modelling of how individual reacts to incentives (including those provided by public policies). PMID:24553852

  10. Evidence on public policy: methodological issues, political issues and examples.

    PubMed

    Attanasio, Orazio P

    2014-03-01

    In this paper I discuss how evidence on public policy is generated and in particular the issue of evaluation of public policies. In economics, the issue of attribution and the identification of causal links has recently received considerable attention. Important methodological issues have been tackled and new techniques have been proposed and used. Randomized Control Trials have become some sort of gold standard. However, they are not exempt from problems and have important limitations: in some case they cannot be constructed and, more generally, problems of external validity and transferability of results can be important. The paper then moves on to discuss the political economy of policy evaluations for policy evaluations to have an impact for the conduct of actual policy, it is important that the demand for evaluation comes directly from the policy making process and is generated endogenously within it. In this sense it is important that the institutional design of policy making is such that policy making institutions are incentivized to use rigorous evaluation in the process of designing policies and allocating resources to alternative options. Economists are currently involved in the design and evaluation of many policies, including policies about health, nutrition and education. The role they can play in these fields is not completely obvious. The paper argues that their main contribution is in the modelling of how individual reacts to incentives (including those provided by public policies).

  11. Fiber optic communication links

    SciTech Connect

    Meyer, R. H.

    1980-01-01

    Fiber optics is a new, emerging technology which offers relief from many of the problems which limited past communications links. Its inherent noise immunity and high bandwidth open the door for new designs with greater capabilities. Being a new technology, certain problems can be encountered in specifying and installing a fiber optic link. A general fiber optic system is discussed with emphasis on the advantages and disadvantages. It is not intended to be technical in nature, but a general discussion. Finally, a general purpose prototype Sandia communications link is presented.

  12. Internet over a Bi-Directional Satellite Link

    NASA Technical Reports Server (NTRS)

    Griner, Jim; Allman, Mark; Mallasch, Paul; Stewart, David

    1998-01-01

    Various issues associated with "Internet over a Bi-Directional Satellite Link" are presented in viewgraph form. Specific topics include: 1) Comarison of HTTP over several network channels; 2) Improved performance of HTTP when compared to off-the-shelf software; 3) Demonstration setup of the link between Sheraton Airport Hotel, Dulles and NASA LeRC; and 4) HTTP comparison pages.

  13. Issues in Teaching Mathematics

    ERIC Educational Resources Information Center

    Ediger, Marlow

    2013-01-01

    In this article, the author states that there are selected issues in mathematics instruction that educators should be well aware of when planning lessons and units of study. These issues provide a basis for thought and discussion when assisting pupils to attain more optimally. Purposeful studying of issues guides mathematics teachers in…

  14. Contemporary American Issues.

    ERIC Educational Resources Information Center

    Lewis, Bill; And Others

    This nine-week unit from a junior high American government curriculum covers the following topics: (1) What are the current issues in our physical and social environment?; (2) How do we investigate and evaluate issues?; and (3) What contemporary issues in my physical or social environment do I want to investigate? Students are required to do…

  15. Precipitation links (PrecipLinks) - a prototype directory for precipitation information

    NASA Technical Reports Server (NTRS)

    Velanthapillia, Balendran; Stocker, Erich Franz

    2006-01-01

    This poster describes a web directory of research oriented precipitation links. In this era of sophisticated search engines and web agents, it might seem counterproductive to establish such a directory of links. However, entering precipitation into a search engine like google will yield over one million hits. To further exacerbate this situation many of the returned links are dead, duplicates of other links, incomplete, or only marginally related to research precipitation or even the broader precipitation area. Sometimes connecting the linked URL causes the browser to lose context and not be able to get back to the original page. Even using more sophisticated search engines query parameters or agents while reducing the overall return doesn't eliminate all of the other issues listed. As part of the development of the measurement-based Precipitation Processing System (PPS) that will support Tropical Rainfall Measuring Mission (TRMM) version 7 reprocessing and the Global Precipitation Measurement (GPM) mission a precipitation links (PrecipLinks) facility is being developed. PrecipLinks is intended to share locations of other sites that contain information or data pertaining to precipitation research. Potential contributors can log-on to the PrecipLinks website and register their site for inclusion in the directory. The price for inclusion is the requirement to place a link back to PrecipLinks on the webpage that is registered. This ensures that users will be able to easily get back to PrecipLinks regardless of any context issues that browsers might have. Perhaps more importantly users while visiting one site that they know can be referred to a location that has many others sites with which they might not be familiar. PrecipLinks is designed to have a very flat structure. This poster summarizes these categories (information, data, services) and the reasons for their selection. Providers may register multiple pages to which they wish to direct users. However, each page

  16. Differences in methylation patterns in the methylation boundary region of IDS gene in Hunter syndrome patients: implications for CpG hot spot mutations.

    PubMed

    Tomatsu, Shunji; Sukegawa, Kazuko; Trandafirescu, Georgeta G; Gutierrez, Monica A; Nishioka, Tatsuo; Yamaguchi, Seiji; Orii, Tadao; Froissart, Roseline; Maire, Irene; Chabas, Amparo; Cooper, Alan; Di Natale, Paola; Gal, Andreas; Noguchi, Akihiko; Sly, William S

    2006-07-01

    Hunter syndrome, an X-linked disorder, results from deficiency of iduronate-2-sulfatase (IDS). Around 40% of independent point mutations at IDS were found at CpG sites as transitional events. The 15 CpG sites in the coding sequences of exons 1 and 2, which are normally hypomethylated, account for very few of transitional mutations. By contrast, the CpG sites in the coding sequences of exon 3, though also normally hypomethylated, account for much higher fraction of transitional mutations. To better understand relationship between methylation status and CpG transitional mutations in this region, the methylation patterns of 11 Hunter patients with transitional mutations at CpG sites were investigated using bisulfite genomic sequencing. The patient cohort mutation spectrum is composed of one mutation in exon 1 (one patient) and three different mutations in exon 3 (10 patients). We confirmed that in normal males, cytosines at the CpG sites from the promoter region to a portion of intron 3 were hypomethylated. However, specific CpG sites in this area were more highly methylated in patients. The patients with p.R8X (exon 1), p.P86L (exon 3), and p.R88H (exon 3) mutations had a hypermethylated condition in exon 2 to intron 3 but retained hypomethylation in exon 1. The same trend was found in four patients with p.A85T (exon 3), although the degree of hypermethylation was less. These findings suggest methylation patterns in the beginning of IDS genomic region are polymorphic in humans and that hypermethylation in this region in some individuals predisposes them to CpG mutations resulting in Hunter syndrome.

  17. The NEC Link Scheme

    ERIC Educational Resources Information Center

    Noakes, Peter

    1976-01-01

    Describes the operation of the National Electronics Council (NEC) Link Scheme for schools in Great Britain. The service is intended to provide technical assistance, information concerning surplus equipment, and guest speakers for school aspiring professional electronic counsel. (CP)

  18. The Link: Business, Education.

    ERIC Educational Resources Information Center

    AGB Reports, 1984

    1984-01-01

    An excerpt from "Corporate and Campus Cooperation: An Action Agenda" (a report from the Business-Higher Education Forum) explains some methods for strengthening the link between the business and education communities. (Author/MLW)

  19. Linked for Learning.

    ERIC Educational Resources Information Center

    Freeman, Laurie

    1996-01-01

    The Northern Tier Distance Learning Consortium links six high schools and three colleges in northeastern Pennsylvania. Indiana students participated in an interactive distance-learning program that focused on underwater exploration. (MLF)

  20. Ethical issues in limb transplants.

    PubMed

    Dickenson, D; Widdershoven, G

    2001-04-01

    On one view, limb transplants cross technological frontiers but not ethical ones; the only issues to be resolved concern professional competence, under the assumption of patient autonomy. Given that the benefits of limb transplant do not outweigh the risks, however, the autonomy and rationality of the patient are not necessarily self-evident. In addition to questions of resource allocation and informed consent, limb, and particularly hand, allograft also raises important issues of personal identity and bodily integrity. We present two linked schemas for exploring ethical issues in limb transplants. The first, relying on conventional concepts in biomedical ethics, asks whether the procedure is research or therapy, whether the costs outweigh the benefits, and whether it should be up to the patient to decide. The second introduces more speculative and theoretically challenging questions, including bodily integrity, the argument from unnaturalness, and the function of the hand in expressing personal identity and intimacy. We conclude that limb transplants are not ruled out a priori, unlike some procedures that are prima facie wrong to perform, such as amputation of healthy limbs to relieve body dysmorphic disorders. However, their legitimacy is not proven by appeals to the interests of scientific research, cost-benefit, or patient autonomy.

  1. Redox-sensitive vascular smooth muscle cell proliferation is mediated by GKLF and Id3 in vitro and in vivo.

    PubMed

    Nickenig, Georg; Baudler, Stephanie; Müller, Cornelius; Werner, Christian; Werner, Nikos; Welzel, Hilke; Strehlow, Kerstin; Böhm, Michael

    2002-07-01

    Reactive oxygen species such as superoxide and hydroxyl radicals have been implicated in the pathogenic growth of various cell types. The molecular mechanisms involved in redox-sensitive cell growth control are poorly understood. Stimulation of cultured vascular smooth muscle cells (VSMC) with xanthin/xanthin oxidase (X/XO) increases proliferation, whereas stimulation with hydrogen peroxide and Fe3+NTA (H-Fe) causes growth arrest of VSMC. Differential Display led to the identification of two novel, differentially regulated redox-sensitive genes. The dominant negative helix-loop-helix protein Id3 is induced by X/XO and down-regulated by H-Fe. The transcription factor gut-enriched Kruppel-like factor (GKLF) is induced by H-Fe but not by X/XO. Induction of GKLF and inhibition of Id3 via transfection experiments leads to growth arrest, whereas overexpression of Id3 and inhibition of GKLF cause cell growth. Id3 down-regulation is induced via binding of GKLF to the Id3 promotor and concomitantly reduced Id3 gene transcription rate. GKLF induction by H-Fe is mediated through hydroxyl radicals, p38MAP kinase-, calcium-, and protein synthesis-dependent pathways. Id3 is induced by X/XO via superoxide, calcium, p38, and p42/44 MAP kinase. GKLF induces and Id3 depresses expression of p21WAF1/Cip1, p27KIP1, p53. Induction of Id3 is accomplished by angiotensin II via superoxide release. A vascular injury mouse model revealed that Id3 is overexpressed in proliferating vascular tissue in vivo. These findings reveal novel mechanisms of redox-controlled cellular proliferation involving GKLF and Id3 that may have general implications for our understanding of vascular and nonvascular growth control.

  2. A Provably Secure Revocable ID-Based Authenticated Group Key Exchange Protocol with Identifying Malicious Participants

    PubMed Central

    Tsai, Tung-Tso

    2014-01-01

    The existence of malicious participants is a major threat for authenticated group key exchange (AGKE) protocols. Typically, there are two detecting ways (passive and active) to resist malicious participants in AGKE protocols. In 2012, the revocable identity- (ID-) based public key system (R-IDPKS) was proposed to solve the revocation problem in the ID-based public key system (IDPKS). Afterwards, based on the R-IDPKS, Wu et al. proposed a revocable ID-based AGKE (RID-AGKE) protocol, which adopted a passive detecting way to resist malicious participants. However, it needs three rounds and cannot identify malicious participants. In this paper, we fuse a noninteractive confirmed computation technique to propose the first two-round RID-AGKE protocol with identifying malicious participants, which is an active detecting way. We demonstrate that our protocol is a provably secure AGKE protocol with forward secrecy and can identify malicious participants. When compared with the recently proposed ID/RID-AGKE protocols, our protocol possesses better performance and more robust security properties. PMID:24991641

  3. Support and Self-Efficacy among Latino and White Parents of Children with ID

    ERIC Educational Resources Information Center

    Cohen, Shana R.; Holloway, Susan D.; Dominguez-Pareto, Irenka; Kuppermann, Miriam

    2015-01-01

    Research indicates that mothers of children with ID who receive familial support experience less stress than those who receive less support. Less is known about the relation of support to mothers' evaluation of parenting self-efficacy, particularly in Latino families. We examined the relationship of different types of family support to life…

  4. OpenID connect as a security service in Cloud-based diagnostic imaging systems

    NASA Astrophysics Data System (ADS)

    Ma, Weina; Sartipi, Kamran; Sharghi, Hassan; Koff, David; Bak, Peter

    2015-03-01

    The evolution of cloud computing is driving the next generation of diagnostic imaging (DI) systems. Cloud-based DI systems are able to deliver better services to patients without constraining to their own physical facilities. However, privacy and security concerns have been consistently regarded as the major obstacle for adoption of cloud computing by healthcare domains. Furthermore, traditional computing models and interfaces employed by DI systems are not ready for accessing diagnostic images through mobile devices. RESTful is an ideal technology for provisioning both mobile services and cloud computing. OpenID Connect, combining OpenID and OAuth together, is an emerging REST-based federated identity solution. It is one of the most perspective open standards to potentially become the de-facto standard for securing cloud computing and mobile applications, which has ever been regarded as "Kerberos of Cloud". We introduce OpenID Connect as an identity and authentication service in cloud-based DI systems and propose enhancements that allow for incorporating this technology within distributed enterprise environment. The objective of this study is to offer solutions for secure radiology image sharing among DI-r (Diagnostic Imaging Repository) and heterogeneous PACS (Picture Archiving and Communication Systems) as well as mobile clients in the cloud ecosystem. Through using OpenID Connect as an open-source identity and authentication service, deploying DI-r and PACS to private or community clouds should obtain equivalent security level to traditional computing model.

  5. The Critical Role of the ID in Interactive Television: The Value of Immediacy.

    ERIC Educational Resources Information Center

    Murphy, Karen L.; Farr, Charlotte W.

    The instructional designer (ID) plays a critical role in the development and viability of each course taught at a distance, particularly in the incorporation of immediacy behaviors, or communication behaviors that convey approachability and communicate interpersonal warmth and closeness. Faculty development must be fostered to encourage immediacy…

  6. Single mimivirus particles intercepted and imaged with an X-ray laser (CXIDB ID 1)

    DOE Data Explorer

    Seibert, M. Marvin; Ekeberg, Tomas; Maia, Filipe R.N.C.

    2011-02-02

    These are the files used to reconstruct the images in the paper "Single Mimivirus particles intercepted and imaged with an X-ray laser". Besides the diffracted intensities, the Hawk configuration files used for the reconstructions are also provided. The files from CXIDB ID 1 are the pattern and configuration files for the pattern showed in Figure 2a in the paper.

  7. Single mimivirus particles intercepted and imaged with an X-ray laser (CXIDB ID 2)

    DOE Data Explorer

    Seibert, M. Marvin; Ekeberg, Tomas

    2011-02-02

    These are the files used to reconstruct the images in the paper "Single Mimivirus particles intercepted and imaged with an X-ray laser". Besides the diffracted intensities, the Hawk configuration files used for the reconstructions are also provided. The files from CXIDB ID 2 are the pattern and configuration files for the pattern showed in Figure 2b in the paper.

  8. Improving the detection of chronic migraine: Development and validation of Identify Chronic Migraine (ID-CM)

    PubMed Central

    Serrano, Daniel; Buse, Dawn C; Pavlovic, Jelena M; Blumenfeld, Andrew M; Dodick, David W; Aurora, Sheena K; Becker, Werner J; Diener, Hans-Christoph; Wang, Shuu-Jiun; Vincent, Maurice B; Hindiyeh, Nada A; Starling, Amaal J; Gillard, Patrick J; Varon, Sepideh F; Reed, Michael L

    2015-01-01

    Background Migraine, particularly chronic migraine (CM), is underdiagnosed and undertreated worldwide. Our objective was to develop and validate a self-administered tool (ID-CM) to identify migraine and CM. Methods ID-CM was developed in four stages. (1) Expert clinicians suggested candidate items from existing instruments and experience (Delphi Panel method). (2) Candidate items were reviewed by people with CM during cognitive debriefing interviews. (3) Items were administered to a Web panel of people with severe headache to assess psychometric properties and refine ID-CM. (4) Classification accuracy was assessed using an ICHD-3β gold-standard clinician diagnosis. Results Stages 1 and 2 identified 20 items selected for psychometric validation in stage 3 (n = 1562). The 12 psychometrically robust items from stage 3 underwent validity testing in stage 4. A scoring algorithm applied to four symptom items (moderate/severe pain intensity, photophobia, phonophobia, nausea) accurately classified most migraine cases among 111 people (sensitivity = 83.5%, specificity = 88.5%). Augmenting this algorithm with eight items assessing headache frequency, disability, medication use, and planning disruption correctly classified most CM cases (sensitivity = 80.6%, specificity = 88.6%). Discussion ID-CM is a simple yet accurate tool that correctly classifies most individuals with migraine and CM. Further testing in other settings will also be valuable. PMID:26002700

  9. 77 FR 56241 - Board Meeting; October 17, 2012; Idaho Falls, ID

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-09-12

    ... From the Federal Register Online via the Government Publishing Office NUCLEAR WASTE TECHNICAL REVIEW BOARD Board Meeting; October 17, 2012; Idaho Falls, ID The U.S. Nuclear Waste Technical Review Board will meet to discuss DOE work on packaging, transporting, and disposing of SNF and HLW. Pursuant to its authority under section 5051 of Public...

  10. Time-Resolved Research at the Advanced Photon Source Beamline 7-ID

    SciTech Connect

    Dufresne, Eric M.; Adams, Bernhard; Arms, Dohn A.; Chollet, Matthieu; Landahl, Eric C.; Li, Yuelin; Walko, Donald A.; Wang, Jin

    2010-08-02

    The Sector 7 undulator beamline (7-ID) of the Advanced Photon Source (APS) is dedicated to time-resolved x-ray research and is capable of ultrafast measurements on the order of 100 ps. Beamline 7-ID has a laser laboratory featuring a Ti:Sapphire system (average power of 2.5W, pulse duration <50 fs, repetition rate 1-5 kHz) that can be synchronized to the bunch pattern of the storage ring. The laser is deliverable to x-ray enclosures, which contain diffractometers, as well as motorized optical tables for table-top experiments. Beamline 7-ID has a single APS Undulator A and uses a diamond (111) double-crystal monochromator, providing good energy resolution over a range of 6-24 keV. Available optics include Kirkpatrick-Baez (KB) mirrors to microfocus the x-ray beam. A variety of time-resolved diffraction and spectroscopy research is available at 7-ID, with experiments being done in the atomic, molecular, optical, chemistry, and solid state (bulk and surface) fields.

  11. Time-Resolved Research at the Advanced Photon Source Beamline 7-ID

    SciTech Connect

    Dufresne, Eric M.; Adams, Bernhard; Arms, Dohn A.; Chollet, Matthieu; Li, Yuelin; Walko, Donald A.; Wang Jin; Landahl, Eric C.

    2010-06-23

    The Sector 7 undulator beamline (7-ID) of the Advanced Photon Source (APS) is dedicated to time-resolved x-ray research and is capable of ultrafast measurements on the order of 100 ps. Beamline 7-ID has a laser laboratory featuring a Ti:Sapphire system (average power of 2.5 W, pulse duration <50 fs, repetition rate 1-5 kHz) that can be synchronized to the bunch pattern of the storage ring. The laser is deliverable to x-ray enclosures, which contain diffractometers, as well as motorized optical tables for table-top experiments. Beamline 7-ID has a single APS Undulator A and uses a diamond (111) double-crystal monochromator, providing good energy resolution over a range of 6-24 keV. Available optics include Kirkpatrick-Baez (KB) mirrors to microfocus the x-ray beam. A variety of time-resolved diffraction and spectroscopy research is available at 7-ID, with experiments being done in the atomic, molecular, optical, chemistry, and solid state (bulk and surface) fields.

  12. OpenID Connect as a security service in cloud-based medical imaging systems.

    PubMed

    Ma, Weina; Sartipi, Kamran; Sharghigoorabi, Hassan; Koff, David; Bak, Peter

    2016-04-01

    The evolution of cloud computing is driving the next generation of medical imaging systems. However, privacy and security concerns have been consistently regarded as the major obstacles for adoption of cloud computing by healthcare domains. OpenID Connect, combining OpenID and OAuth together, is an emerging representational state transfer-based federated identity solution. It is one of the most adopted open standards to potentially become the de facto standard for securing cloud computing and mobile applications, which is also regarded as "Kerberos of cloud." We introduce OpenID Connect as an authentication and authorization service in cloud-based diagnostic imaging (DI) systems, and propose enhancements that allow for incorporating this technology within distributed enterprise environments. The objective of this study is to offer solutions for secure sharing of medical images among diagnostic imaging repository (DI-r) and heterogeneous picture archiving and communication systems (PACS) as well as Web-based and mobile clients in the cloud ecosystem. The main objective is to use OpenID Connect open-source single sign-on and authorization service and in a user-centric manner, while deploying DI-r and PACS to private or community clouds should provide equivalent security levels to traditional computing model. PMID:27340682

  13. 77 FR 55486 - Agency Information Collection Activities: Importer ID Input Record

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-09-10

    ... collection techniques or the use of other forms of information technology; and (e) the annual costs burden to... SECURITY U.S. Customs and Border Protection Agency Information Collection Activities: Importer ID Input... and request for comments; Extension of an existing collection of information. SUMMARY: U.S....

  14. 76 FR 43196 - Implementation of the Truth in Caller ID Act

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-07-20

    ... proposed in the Caller ID Act NPRM, 76 FR 16367, the revised rules also specify that blocking or attempting... manipulating caller identification information with the intent to defraud, cause harm, or wrongfully obtain... information with the intent to defraud, cause harm, or wrongfully obtain anything of value. Synopsis of...

  15. 76 FR 16367 - Implementation of the Truth in Caller ID Act of 2009

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-03-23

    ... Proceedings, 63 FR 24121 (1998). Electronic Filers: Comments may be filed electronically using the Internet by... ID spoofing done with the intent to defraud, cause harm, or wrongfully obtain anything of value. The... information with the intent to defraud, cause harm, or wrongfully obtain anything of value. The Truth...

  16. What's Going on in Your Professor's Head? Demonstrating the Id, Ego, and Superego

    ERIC Educational Resources Information Center

    Segrist, Dan J.

    2009-01-01

    This article describes an in-class activity designed to demonstrate Freud's structural theory of the psyche, specifically the roles of the id, ego, and superego, as well as the interplay among them. Additionally, the activity visually illustrates Freud's ideas about the levels of consciousness associated with these 3 components. Pre-post quiz…

  17. Developing Technical Expertise in Secondary Technical Schools: The Effect of 4C/ID Learning Environments

    ERIC Educational Resources Information Center

    Sarfo, Frederick K.; Elen, Jan

    2007-01-01

    In this study, the effectiveness of learning environments, developed in line with the specifications of the four components instructional design model (4C/ID model) and the additional effect of ICT for fostering the development of technical expertise in traditional Ghanaian classrooms, was assessed. The study had a one-by-one-by-two…

  18. OpenID Connect as a security service in cloud-based medical imaging systems.

    PubMed

    Ma, Weina; Sartipi, Kamran; Sharghigoorabi, Hassan; Koff, David; Bak, Peter

    2016-04-01

    The evolution of cloud computing is driving the next generation of medical imaging systems. However, privacy and security concerns have been consistently regarded as the major obstacles for adoption of cloud computing by healthcare domains. OpenID Connect, combining OpenID and OAuth together, is an emerging representational state transfer-based federated identity solution. It is one of the most adopted open standards to potentially become the de facto standard for securing cloud computing and mobile applications, which is also regarded as "Kerberos of cloud." We introduce OpenID Connect as an authentication and authorization service in cloud-based diagnostic imaging (DI) systems, and propose enhancements that allow for incorporating this technology within distributed enterprise environments. The objective of this study is to offer solutions for secure sharing of medical images among diagnostic imaging repository (DI-r) and heterogeneous picture archiving and communication systems (PACS) as well as Web-based and mobile clients in the cloud ecosystem. The main objective is to use OpenID Connect open-source single sign-on and authorization service and in a user-centric manner, while deploying DI-r and PACS to private or community clouds should provide equivalent security levels to traditional computing model.

  19. Use of false ID cards and other deceptive methods to purchase alcoholic beverages during high school.

    PubMed

    Schwartz, R H; Farrow, J A; Banks, B; Giesel, A E

    1998-01-01

    Altered motor vehicle drivers's licenses or other falsified or counterfeit photo identification cards are widely and illegally used by teenagers to obtain beer and other alcohol beverages. We obtained information on the methods currently used by teenagers to purchase beer and wine by asking nine hundred teenagers, between 16-19 years old to complete a brief, confidential questionnaire. High school students most often obtained alcoholic beverages by requesting someone of legal age to purchase it for them. College students used borrowed, altered, or counterfeit identification (ID) more often than high school students. Photo IDs purchased through mail order from a magazine advertisement were used infrequently and when use was attempted, they were sometimes (25%) unsuccessful. Fifteen percent of high school students, 14% of college freshmen, and 24% of teenage drug abusers were able to purchase beer by the case with borrowed, altered, or fake ID. Suggestions to reduce sales of alcohol-containing beverages to minors include universal "carding" of prospective purchasers, use of two view or hologram photos on a drivers' license, requiring three different ID cards at the point of purchase, and penalties to stores that fail to make a good effort to identify underage customers. PMID:9789157

  20. 76 FR 78940 - Minidoka National Wildlife Refuge, Blaine, Cassia, Minidoka, and Power Counties, ID...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-12-20

    .... The Refuge is located 12 miles northeast of Rupert, ID, in the Snake River Plain, at approximately 4,200 feet in elevation. The area was historically comprised of a portion of the Snake River surrounded... impounded the Snake River and created Lake Walcott to store water for irrigation, and provide...

  1. Suppression of mammary epithelial cell differentiation by the helix-loop-helix protein Id-1

    SciTech Connect

    Desprez, P.; Hara, E.; Bissell, M.J.

    1995-06-01

    Cell proliferation and differentiation are precisely coordinated during the development and maturation of the mammary gland, and this balance invariably is disrupted during carcinogenesis. Little is known about the cell-specific transcription factors that regulate these processes in the mammary gland. The mouse mammary epithelial cell line SCp2 grows well under standard culture conditions but arrests growth, forms alveolus-like structures, and expresses {beta}-casein, a differentiation marker, 4 to 5 days after exposure to basement membrane and lactogenic hormones (differentiation signals). The authors show that this differentiation entails a marked decline in the expression of Id-1, a helix-loop-helix (HLH) protein that inactivates basic HLH transcription factors in other cell types. SCp2 cells stably transfected with an Id-1 expression vector grew more rapidly than control cells under standard conditions, but in response to differentiation signals, they lost three-dimensional organization, invaded the basement membrane, and then resumed growth. SCp2 cells expressing an Id-1 antisense vector grew more slowly than controls; in response to differentiation signals, they remained stably growth arrested and fully differentiated, as did control cells. The authors suggest that Id-1 renders cells refractory to differentiation signals and receptive to growth signals by inactivating one or more basic HLH proteins that coordinate growth and differentiation in the mammary epithelium. 53 refs., 6 figs.

  2. 75 FR 35083 - Notice of Realty Action; Direct Sale of Public Lands in Lincoln County, ID

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-06-21

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF THE INTERIOR Bureau of Land Management Notice of Realty Action; Direct Sale of Public Lands in Lincoln County, ID AGENCY: Bureau of Land Management, Interior. ACTION: Notice of realty action. SUMMARY: The Bureau of...

  3. 76 FR 28306 - Amendment of Class D and Class E Airspace; Idaho Falls, ID

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-05-17

    ... Federal Register a notice of proposed rulemaking to amend controlled airspace at Idaho Falls, ID (76 FR...'' under DOT Regulatory Policies and Procedures (44 FR 11034; February 26, 1979); and (3) does not warrant... continues to read as follows: Authority: 49 U.S.C. 106(g), 40103, 40113, 40120; E. O. 10854, 24 FR 9565,...

  4. 76 FR 25537 - Amendment of Class E Airspace; McCall, ID

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-05-05

    ... controlled airspace at McCall, ID (76 FR 8324). Interested parties were invited to participate in this... Executive Order 12866; (2) is not a ``significant rule'' under DOT Regulatory Policies and Procedures (44 FR..., 40113, 40120; E.O. 10854, 24 FR 9565, 3 CFR, 1959-1963 Comp., p. 389. Sec. 71.1 0 2. The...

  5. 75 FR 15741 - Applied Materials; Boise, ID; Notice of Termination of Investigation

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-03-30

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF LABOR Employment and Training Administration Applied Materials; Boise, ID; Notice of Termination of Investigation Pursuant to Section 221 of the Trade Act of 1974, as amended, an investigation was initiated in response...

  6. 76 FR 9266 - Proposed Amendment of Class D and Class E Airspace; Idaho Falls, ID

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-02-17

    ...'' under DOT Regulatory Policies and Procedures (44 FR 11034; February 26, 1979); and (3) does not warrant.... 10854, 24 FR 9565, 3 CFR, 1959-1963 Comp., p. 389. Sec. 71.1 2. The incorporation by reference in 14 CFR... Falls, ID AGENCY: Federal Aviation Administration (FAA), DOT. ACTION: Notice of proposed...

  7. Extraction of words from the national ID cards for automated recognition

    NASA Astrophysics Data System (ADS)

    Akhter, Md. Rezwan; Bhuiyan, Md. Hasanuzzaman; Uddin, Mohammad Shorif

    2011-10-01

    The government of Bangladesh introduced national ID cards in 2008 for all peoples of age 18 years and above. This card is now a de-facto identity document and finds diverse applications in vote casting, bank account opening, telephone subscribing as well as in many real life transactions and security checking. To get real fruits of this versatile ID card, automated retrieving and recognition of an independent person from this extra large national database is an ultimate necessity. This work is the first step to fill this gap in making the recognition in automated fashion. Here we have investigated an image analysis technique to extract the words that will be used in subsequent recognition steps. At first scanned ID card image is used as an input into the computer system and then the target text region is separated from the picture region. The text region is used for separation of lines and words on the basis of the vertical and horizontal projections of image intensity, respectively. Experimentation using real national ID cards confirms the effectiveness of our technique.

  8. 75 FR 11936 - Hewlett Packard; Technical Support Call Center; Boise, ID; Notice of Termination of Investigation

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-03-12

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF LABOR Employment and Training Administration Hewlett Packard; Technical Support Call Center; Boise, ID; Notice of... Packard, Technical Support Call Center, Boise, Idaho. The petitioner has requested that the petition...

  9. Coupling Molecular Modeling to the Traditional "IR-ID" Exercise in the Introductory Organic Chemistry Laboratory

    ERIC Educational Resources Information Center

    Stokes-Huby, Heather; Vitale, Dale E.

    2007-01-01

    This exercise integrates the infrared unknown identification ("IR-ID") experiment common to most organic laboratory syllabi with computer molecular modeling. In this modification students are still required to identify unknown compounds from their IR spectra, but must additionally match some of the absorptions with computed frequencies they…

  10. 75 FR 47515 - Identification (ID) Cards for Members of the Uniformed Services, Their Dependents, and Other...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-08-06

    ... for common access card (CAC) life-cycle compliance with Federal Information Processing Standards (FIPS...(AT&L), establish policy and oversight for CAC life-cycle compliance with FIPS Publication 201-1. (2... eligibility through DEERS. (2) Develop and implement Component-level ID card life-cycle procedures to...

  11. 78 FR 9029 - Nez Perce-Clearwater National Forests; ID; Clear Creek Integrated Restoration Project

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-02-07

    ... Forest Service Nez Perce-Clearwater National Forests; ID; Clear Creek Integrated Restoration Project... Creek Integrated Restoration Draft Environmental Impact Statement (DEIS). The DEIS will include two site... adopt the Regional soils standard for the Clear Creek Integrated Restoration project area. The...

  12. 78 FR 40382 - Modification of Class D and E Airspace; Twin Falls, ID

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-07-05

    ... Falls Joslin Field-Magic Valley Regional Airport, Twin Falls, ID (78 FR 25406). Interested parties were... Executive Order 12866; (2) is not a ``significant rule'' under DOT Regulatory Policies and Procedures (44 FR... read as follows: Authority: 49 U.S.C. 106(g), 40103, 40113, 40120; E. O. 10854, 24 FR 9565, 3 CFR,...

  13. 77 FR 13072 - Salmon-Challis National Forest, Butte, Custer and Lemhi Counties, ID, Supplemental Environmental...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-03-05

    ... Forest Service Salmon-Challis National Forest, Butte, Custer and Lemhi Counties, ID, Supplemental Environmental Impact Statement to the 2009 Salmon- Challis National Forest Travel Planning and OHV Route... environmental impact statement. SUMMARY: The Salmon-Challis National Forest announces its intent to prepare...

  14. The Id, Ego and Super-Ego in "Pride and Prejudice"

    ERIC Educational Resources Information Center

    Liang, Yamin

    2011-01-01

    This paper mainly analyses the the id, ego, and super-ego which exists in the main character Elizabeth from several aspects, such as her pursuit for love, her prejudice towards Mr. Darcy, and the changes in her attitudes towards Wickham. This analysis helps readers appreciate this masterpiece from a different aspect which is related to the…

  15. National Plant Diagnostic Network, Taxonomic training videos: Introduction to AphID

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Training is a critical part of aphid (Hemiptera: Aphididae) identification. This video provides visual instruction on the use of the expert system, AphID, for aphid examination and identification. The video demonstrates the use of different training modules that allow the user to gain familiarity wi...

  16. 75 FR 22695 - Establishment of Class E Airspace; Bonners Ferry, ID

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-04-30

    ... Register a notice of proposed rulemaking to amend controlled airspace at Bonners Ferry, ID (74 FR 58570... Regulatory Policies and Procedures (44 FR 11034; February 26, 1979); and (3) does not warrant preparation of... continues to read as follows: Authority: 49 U.S.C. 106(g), 40103, 40113, 40120; E.O. 10854, 24 FR 9565,...

  17. An Integral ASIE ID Model: The 21st Century Instructional Design Model for Teachers

    ERIC Educational Resources Information Center

    Zain, Ismail Md.; Muniandy, Balakrishnan; Hashim, Wahid

    2016-01-01

    Design of instruction is an important feature in teacher education at fulfilling the needs of 4Cs ("critical thinker," "communicator," "collaborator," "creator") developing "a globally competitive learners". As Instructional design models (ID) need to move from adopting a standard approach to…

  18. Epigenetic regulation of ID4 in the determination of the BRCAness phenotype in breast cancer.

    PubMed

    Branham, M T; Campoy, E; Laurito, S; Branham, R; Urrutia, G; Orozco, J; Gago, F; Urrutia, R; Roqué, M

    2016-01-01

    BRCAness breast tumors represent a group of sporadic tumors characterized by a reduction in BRCA1 gene expression. As BRCA1 is involved in double-strand breaks (DSBs) repair, dysfunctional BRCA pathway could make a tumor sensitive to DNA damaging drugs (e.g., platinum agents). Thus, accurately identifying BRCAness could contribute to therapeutic decision making in patients harboring these tumors. The purpose of this study was to identify if BRCAness tumors present a characteristic methylation profile and/or were related to specific clinico-pathological features. BRCAness was measured by MLPA in 63 breast tumors; methylation status of 98 CpG sites within 84 cancer-related genes was analyzed by MS-MLPA. Protein and mRNA expressions of the selected genes were measured by quantitative real-time PCR and Western Blot. BRCAness was associated with younger age, higher nuclear pleomorphism, and triple-negative (TN) status. Epigenetically, we found that the strongest predictors for BRCAness tumors were the methylations of MLH1 and PAX5 plus the unmethylations of CCND2 and ID4. We determined that ID4 unmethylation correlated with the expression levels of both its mRNA and protein. We observed an inverse relation between the expressions of ID4 and BRCA1. To the best of our knowledge, this is the first report suggesting an epigenetic regulation of ID4 in BRCAness tumors. Our findings give new information of BRCAness etiology and encourage future studies on potential drug targets for BRCAness breast tumors.

  19. Use of false ID cards and other deceptive methods to purchase alcoholic beverages during high school.

    PubMed

    Schwartz, R H; Farrow, J A; Banks, B; Giesel, A E

    1998-01-01

    Altered motor vehicle drivers's licenses or other falsified or counterfeit photo identification cards are widely and illegally used by teenagers to obtain beer and other alcohol beverages. We obtained information on the methods currently used by teenagers to purchase beer and wine by asking nine hundred teenagers, between 16-19 years old to complete a brief, confidential questionnaire. High school students most often obtained alcoholic beverages by requesting someone of legal age to purchase it for them. College students used borrowed, altered, or counterfeit identification (ID) more often than high school students. Photo IDs purchased through mail order from a magazine advertisement were used infrequently and when use was attempted, they were sometimes (25%) unsuccessful. Fifteen percent of high school students, 14% of college freshmen, and 24% of teenage drug abusers were able to purchase beer by the case with borrowed, altered, or fake ID. Suggestions to reduce sales of alcohol-containing beverages to minors include universal "carding" of prospective purchasers, use of two view or hologram photos on a drivers' license, requiring three different ID cards at the point of purchase, and penalties to stores that fail to make a good effort to identify underage customers.

  20. 17 CFR 239.63 - Form ID, uniform application for access codes to file on EDGAR.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ...: For Federal Register citations affecting Form ID, see the List of CFR Sections Affected, which appears... has been authorized by the filer. (c) Password (PW)—allows a filer, filing agent or training agent to log on to the EDGAR system, submit filings, and change its CCC. (d) Password...

  1. 17 CFR 249.446 - Form ID, uniform application for access codes to file on EDGAR.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ...: For Federal Register citations affecting Form ID, see the List of CFR Sections Affected, which appears... has been authorized by the filer. (c) Password (PW)—allows a filer, filing agent or training agent to log on to the EDGAR system, submit filings, and change its CCC. (d) Password...

  2. 17 CFR 239.63 - Form ID, uniform application for access codes to file on EDGAR.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ...: For Federal Register citations affecting Form ID, see the List of CFR Sections Affected, which appears... has been authorized by the filer. (c) Password (PW)—allows a filer, filing agent or training agent to log on to the EDGAR system, submit filings, and change its CCC. (d) Password...

  3. 17 CFR 269.7 - Form ID, uniform application for access codes to file on EDGAR.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... Federal Register citations affecting Form ID, see the List of CFR Sections Affected, which appears in the... been authorized by the filer. (c) Password (PW)—allows a filer, filing agent or training agent to log on to the EDGAR system, submit filings, and change its CCC. (d) Password Modification...

  4. 17 CFR 269.7 - Form ID, uniform application for access codes to file on EDGAR.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... Federal Register citations affecting Form ID, see the List of CFR Sections Affected, which appears in the... been authorized by the filer. (c) Password (PW)—allows a filer, filing agent or training agent to log on to the EDGAR system, submit filings, and change its CCC. (d) Password Modification...

  5. 17 CFR 249.446 - Form ID, uniform application for access codes to file on EDGAR.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ...: For Federal Register citations affecting Form ID, see the List of CFR Sections Affected, which appears... has been authorized by the filer. (c) Password (PW)—allows a filer, filing agent or training agent to log on to the EDGAR system, submit filings, and change its CCC. (d) Password...

  6. 77 FR 50417 - Proposed Amendment of Class D and Class E Airspace; Lewiston, ID

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-08-21

    ... your comments. You may also submit comments through the Internet at http://www.regulations.gov . FOR..., ID (77 FR 32921). Also the geographic coordinates of the airport and navigation aids would be... phone number). You may also submit comments through the Internet at...

  7. 78 FR 51677 - Children's Online Privacy Protection Rule Proposed Parental Consent Method; AssertID, Inc...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-08-21

    ... meets the requirements for parental consent described in 16 CFR 312.5(b)(1). \\1\\ 64 FR 59888 (1999). \\2\\ 16 CFR part 312. \\3\\ 78 FR 3972 (2013). \\4\\ 16 CFR 312.12(a); 78 FR at 3991-3992, 4013. Pursuant to... Parental Consent Method; AssertID, Inc. Application for Approval of Parental Consent Method AGENCY:...

  8. 78 FR 61442 - Idaho Disaster #ID-00027 Declaration of Economic Injury

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-10-03

    ... ADMINISTRATION Idaho Disaster ID-00027 Declaration of Economic Injury AGENCY: U.S. Small Business Administration. ACTION: Notice. SUMMARY: This is a notice of an Economic Injury Disaster Loan (EIDL) declaration for the... the Administrator's EIDL declaration, applications for economic injury disaster loans may be filed...

  9. Certification of Completion of Item 2 of ASC FY07 Level-2 Milestone ID #2380

    SciTech Connect

    Lipari, D A

    2007-03-28

    This report documents the completion of Item 2 of the three milestone deliverables that comprise Milestone ID 2380: Deploy selected Tri-Lab resource manager at LLNL and develop support model. Specifically: LLNL will integrate and support a commercial resource manager software product at LLNL to be used across the tri-lab HPC facilities.

  10. 75 FR 45661 - Notice of Permanent Closure on Public Lands in Ada County, ID

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-08-03

    ... Bureau of Land Management Notice of Permanent Closure on Public Lands in Ada County, ID AGENCY: Bureau of Land Management, Interior. ACTION: Notice of permanent closure. SUMMARY: On April 12, 2010, Higby Cave and all public lands within 1,000 feet of the entrance were permanently closed to vehicle access...

  11. 75 FR 41773 - Proposed Amendment of Class E Airspace; Arco, ID

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-07-19

    ... ``significant rule'' under DOT Regulatory Policies and Procedures (44 FR 11034; February 26, 1979); and (3) does..., 40113, 40120; E.O. 10854, 24 FR 9565, 3 CFR, 1959-1963 Comp., p. 389. Sec. 71.1 2. The incorporation by... Federal Aviation Administration 14 CFR Part 71 Proposed Amendment of Class E Airspace; Arco, ID...

  12. 78 FR 39767 - Notice of Realty Action: Direct Sale of Public Land in Blaine County, ID

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-07-02

    ... sale. The BLM has completed a mineral potential report which concluded there ] are no known mineral values in the lands proposed for sale. The BLM proposes that conveyance of the Federal mineral interests... Bureau of Land Management Notice of Realty Action: Direct Sale of Public Land in Blaine County, ID...

  13. Publications of the School-Linked Services Integration Research Project.

    ERIC Educational Resources Information Center

    Kansas Univ., Lawrence.

    This compilation of publications from the School-Linked Service Integration Research Project focuses on integrated services and includes the following papers: (1) "New Community Schools: Issues for Families in Three Streams of Reform" (Wayne Sailor), describes issues in special education reform, general education reform, and school/health services…

  14. Linking Resources to Learning: Conceptual and Practical Problems.

    ERIC Educational Resources Information Center

    Wilkins, Raphael

    2002-01-01

    Investigates resourcing issues for schools through headteachers' perceptions of the funding issues. Delegation of financial decision-making has made it harder to obtain impartial analyses of resource-deployment decisions. Concludes that linking resources to learning requires a shift in emphasis away from apportionment and toward the language of…

  15. [Identification of a novel mutation of IDS gene from a Chinese pedigree with MPS II].

    PubMed

    GUO, Yi-Bin; PAN, Hong-Da; GUO, Chun-Miao; LI, Yong-Mei; CHEN, Lu-Ming

    2009-11-01

    The purpose of this study was to understand the molecular genetic mechanism of mucopolysaccharidosis type II (MPS II) and to provide a prerequisite for future prenatal gene diagnosis. A preliminary diagnosis was made by qualitative detection of Urinary Glycosaminoglycans of the suspected MPS II proband. Then, mutation detection was performed on the proband and his family members with PCR and direct sequencing of PCR products. After the novel mutation of c.876 del 2 in IDS gene was detected, sequence analysis was performed on exon 6 of IDS gene of the 135 cases, which consisted of 120 randomly selected normal controls, and other 15 patients with MPS I, IV, and VI other than MPS II. Besides, the patho-genicity of the novel mutation was analyzed with the following 2 methods: conservative analysis of the sequence of muta-tion spots of different species and the direct test of the IDS enzyme activity of the patient and his relative family members. The result of uroscopy of the proband was strong positive (GAGs +++). There was a novel deletion mutation of c.876-877 del TC in the coding region of exon 6 of IDS gene, which was a hemizygous mutation. However, the mutation of his mother and sister was a heterozygous mutation. Detection of the exon 6 of IDS gene showed that the mutation was not found among normal controls and other patients with MPS I, IV, and VI other than MPS II. Homology comparison of amino acid sequences from different species showed that the phenylalanine (F) glutamine (Q) of the mutation site of c.876-877 del TC located in p.292-293 was highly conserved. The activity of IDS enzyme of the proband was only 2.3 nmol/4 h/mL, which was much lower than normal; but the activity of IDS enzyme of his father, mother and sister was 641.9 nmol/4 h/mL, 95.8 nmol/4h/mL and 103.2 nmol/4h/mL, respectively. These results illustrated that the deletion and frame-shift mutation of c.876-877 del TC detected was a novel pathologic mutation, which was the underlying cause of

  16. Data link communications in the National Airspace System

    NASA Technical Reports Server (NTRS)

    Lee, Alfred T.

    1989-01-01

    In the near future, conventional radio communications, currently the primary medium for the transfer of information between aircraft and ground stations, will be replaced by digital data link. This paper briefly describes this technology and summarizes what are believed to be the principal human factor issues associated with data link implementation in the airspace system. Integration of data link communications with existing systems on the flight deck and in the Air Traffic Control system is discussed with regard for both near term implementation and longer term operational issues.

  17. Linked Ocean Data

    NASA Astrophysics Data System (ADS)

    Leadbetter, Adam; Arko, Robert; Chandler, Cynthia; Shepherd, Adam

    2014-05-01

    "Linked Data" is a term used in Computer Science to encapsulate a methodology for publishing data and metadata in a structured format so that links may be created and exploited between objects. Berners-Lee (2006) outlines the following four design principles of a Linked Data system: Use Uniform Resource Identifiers (URIs) as names for things. Use HyperText Transfer Protocol (HTTP) URIs so that people can look up those names. When someone looks up a URI, provide useful information, using the standards (Resource Description Framework [RDF] and the RDF query language [SPARQL]). Include links to other URIs so that they can discover more things. In 2010, Berners-Lee revisited his original design plan for Linked Data to encourage data owners along a path to "good Linked Data". This revision involved the creation of a five star rating system for Linked Data outlined below. One star: Available on the web (in any format). Two stars: Available as machine-readable structured data (e.g. An Excel spreadsheet instead of an image scan of a table). Three stars: As two stars plus the use of a non-proprietary format (e.g. Comma Separated Values instead of Excel). Four stars: As three stars plus the use of open standards from the World Wide Web Commission (W3C) (i.e. RDF and SPARQL) to identify things, so that people can point to your data and metadata. Five stars: All the above plus link your data to other people's data to provide context Here we present work building on the SeaDataNet common vocabularies served by the NERC Vocabulary Server, connecting projects such as the Rolling Deck to Repository (R2R) and the Biological and Chemical Oceanography Data Management Office (BCO-DMO) and other vocabularies such as the Marine Metadata Interoperability Ontology Register and Repository and the NASA Global Change Master Directory to create a Linked Ocean Data cloud. Publishing the vocabularies and metadata in standard RDF XML and exposing SPARQL endpoints renders them five-star Linked

  18. Chronic kidney disease in older people with intellectual disability: results of the HA-ID study.

    PubMed

    de Winter, C F; Echteld, M A; Evenhuis, H M

    2014-03-01

    With increasing longevity and cardiovascular events, chronic kidney disease may also become a significant problem in older people with intellectual disability (ID). We studied prevalence and associations of chronic kidney disease as part of the Healthy Ageing and Intellectual Disability (HA-ID) study, a large Dutch cross-sectional study among people with ID aged 50 years and over, using creatinine and cystatin-C measurement in plasma. Glomerular filtration rate (GFR) was calculated using the Modification of Diet in Renal Disease (MDRD) and Chronic Kidney Disease Epidemiology Collaboration (CKD-EPI) equations. Equations based on creatinine (as the MDRD equation) may underestimate kidney dysfunction in people with sarcopenia, because low muscle mass leads to a low creatinine production. Therefore, also prevalence of chronic kidney disease was studied in the sarcopenic group, using different GFR equations. Prevalence of chronic kidney disease, among 635 participants, was 15.3%, which equals prevalence in the general Dutch population. In the group of participants with sarcopenia (n=82), the CKD-EPI equation based on creatinine and cystatin-C gave a higher prevalence of chronic kidney disease than did the MDRD equation, but confidence intervals were very wide. Chronic kidney disease was associated with higher age, Down syndrome, obesity, hypercholesterolemia and hypothyroid disease. GFR should be measured in all older people with ID and polypharmacy, and in older people with ID and Down syndrome as part of the regular health checks. Moreover, if sarcopenia is present and information on GFR is required, this should not be measured based on creatinine only, but additional measures, such as cystatin-C, should be taken into account.

  19. 78 FR 9446 - Advance Nanotech, Inc., Advanced ID Corp., Aeon Holdings, Inc. (n/k/a BCM Energy Partners, Inc...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-02-08

    ... COMMISSION Advance Nanotech, Inc., Advanced ID Corp., Aeon Holdings, Inc. (n/k/a BCM Energy Partners, Inc..., Ltd., EcoReady Corp., EnDevCo, Inc., Electronic Kourseware International, Inc., Ensign Services, Inc... accurate information concerning the securities of Advanced ID Corp. because it has not filed any...

  20. Performance of Candida ID, a New Chromogenic Medium for Presumptive Identification of Candida Species, in Comparison to CHROMagar Candida

    PubMed Central

    Willinger, Birgit; Hillowoth, Cornelia; Selitsch, Brigitte; Manafi, Mammad

    2001-01-01

    Candida ID agar allows identification of Candida albicans and differentiation of other Candida species. In comparison with CHROMagar Candida, we evaluated the performance of this medium directly from 596 clinical specimens. In particular, detection of C. albicans after 24 h of incubation was easier on Candida ID (sensitivity, 96.8%) than on CHROMagar (sensitivity, 49.6%). PMID:11574621

  1. Health Professionals' Attitudes and Emotions towards Working with Adults with Intellectual Disability (ID) and Mental Ill Health

    ERIC Educational Resources Information Center

    Rose, N.; Kent, S.; Rose, J.

    2012-01-01

    Objectives: Mainstream mental health services are providing more care for individuals with an intellectual disability (ID); this has implications for staff and service users. Attitudes of staff towards people with ID in mental health services may be negative and negative staff attitudes may have a detrimental impact on service provision. Design: A…

  2. 77 FR 55800 - Foreign-Trade Zone 242-Boundary County, ID; Application for Subzone AREVA Enrichment Services...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-09-11

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF COMMERCE Foreign-Trade Zones Board Foreign-Trade Zone 242--Boundary County, ID; Application for Subzone AREVA Enrichment Services, LLC; Bonneville County, ID An application has been submitted to the Foreign-Trade...

  3. Limited Concordance between Teachers, Parents and Healthcare Professionals on the Presence of Chronic Diseases in ID-Adolescents

    ERIC Educational Resources Information Center

    Oeseburg, B.; Jansen, D. E. M. C.; Reijneveld, S. A.; Dijkstra, G. J.; Groothoff, J. W.

    2010-01-01

    Evidence on teachers' knowledge about somatic and mental chronic diseases among ID-adolescent compared to the knowledge parents and healthcare professionals have, is limited. The aim of this study is: (1) to assess the knowledge of teachers on the presence of chronic diseases in ID-adolescents; (2) to compare teachers with parents and healthcare…

  4. Id1 expression promotes peripheral CD4{sup +} T cell proliferation and survival upon TCR activation without co-stimulation

    SciTech Connect

    Liu, Chen; Jin, Rong; Wang, Hong-Cheng; Tang, Hui; Liu, Yuan-Feng; Qian, Xiao-Ping; Sun, Xiu-Yuan; Ge, Qing; Sun, Xiao-Hong; Zhang, Yu

    2013-06-21

    Highlights: •Id1 expression enables naïve T cell proliferation without anti-CD28 co-stimulation. •Id1 expression facilitates T cells survival when stimulated with anti-CD3. •Elevation of IL-2 production by Id1 contributes increased proliferation and survival. •Id1 potentiates NF-κB activation by anti-CD3 stimulation. -- Abstract: Although the role of E proteins in the thymocyte development is well documented, much less is known about their function in peripheral T cells. Here we demonstrated that CD4 promoter-driven transgenic expression of Id1, a naturally occurring dominant-negative inhibitor of E proteins, can substitute for the co-stimulatory signal delivered by CD28 to facilitate the proliferation and survival of naïve CD4{sup +} cells upon anti-CD3 stimulation. We next discovered that IL-2 production and NF-κB activity after anti-CD3 stimulation were significantly elevated in Id1-expressing cells, which may be, at least in part, responsible for the augmentation of their proliferation and survival. Taken together, results from this study suggest an important role of E and Id proteins in peripheral T cell activation. The ability of Id proteins to by-pass co-stimulatory signals to enable T cell activation has significant implications in regulating T cell immunity.

  5. Initial Validation of the Chinese Quality of Life Questionnaire-Intellectual Disabilities (CQOL-ID): A Cultural Perspective

    ERIC Educational Resources Information Center

    Wong, P. K. S.; Wong, D. F. K.; Schalock, R. L.; Chou, Y-C.

    2011-01-01

    Background: In the field of intellectual disabilities (ID), the quality of life concept has been developing rapidly in Chinese societies including Hong Kong, mainland China and Taiwan. However, there is a lack of locally validated instruments to measure the quality of life of people with ID. The study reported in this paper attempted to validate…

  6. Aviation Safety Issues Database

    NASA Technical Reports Server (NTRS)

    Morello, Samuel A.; Ricks, Wendell R.

    2009-01-01

    The aviation safety issues database was instrumental in the refinement and substantiation of the National Aviation Safety Strategic Plan (NASSP). The issues database is a comprehensive set of issues from an extremely broad base of aviation functions, personnel, and vehicle categories, both nationally and internationally. Several aviation safety stakeholders such as the Commercial Aviation Safety Team (CAST) have already used the database. This broader interest was the genesis to making the database publically accessible and writing this report.

  7. Current issues and actions

    SciTech Connect

    Black, D.G.

    1995-06-01

    This section of the 1994 Hanford Site Environmental Report summarizes the progress that has been made toward achieving full regulatory compliance at the Hanford Site. Ongoing compliance self-assessments, implementation of the Tri-Party Agreement, and public meetings continue to identify environmental compliance issues. These issues are discussed openly with the regulatory agencies and with the public to ensure that all environmental compliance issues are addressed.

  8. ISSUES MANAGEMENT PROGRAM MANUAL

    SciTech Connect

    Gravois, Melanie

    2007-06-27

    The Lawrence Berkeley National Laboratory (LBNL) Issues Management Program encompasses the continuous monitoring of work programs, performance and safety to promptly identify issues to determine their risk and significance, their causes, and to identify and effectively implement corrective actions to ensure successful resolution and prevent the same or similar problems from occurring. This document describes the LBNL Issues Management Program and prescribes the process for issues identification, tracking, resolution, closure, validation, and effectiveness of corrective actions. Issues that are governed by this program include program and performance deficiencies or nonconformances that may be identified through employee discovery, internal or external oversight assessment findings, suggested process improvements and associated actions that require formal corrective action. Issues may also be identified in and/or may result in Root Cause Analysis (RCA) reports, Price Anderson Amendment Act (PAAA) reports, Occurrence Reporting and Processing System (ORPS) reports, Accident Investigation reports, assessment reports, and External Oversight reports. The scope of these issues may include issues of both high and low significance as well as adverse conditions that meet the reporting requirements of the University of California (UC) Assurance Plan for LBNL or other reporting entities (e.g., U.S. Environmental Protection Agency, U.S. Department of Energy). Issues that are found as a result of a walk-around or workspace inspection that can be immediately corrected or fixed are exempt from the requirements of this document.

  9. Data issues in the life sciences

    PubMed Central

    Thessen, Anne E.; Patterson, David J.

    2011-01-01

    Abstract We review technical and sociological issues facing the Life Sciences as they transform into more data-centric disciplines - the “Big New Biology”. Three major challenges are: 1) lack of comprehensive standards; 2) lack of incentives for individual scientists to share data; 3) lack of appropriate infrastructure and support. Technological advances with standards, bandwidth, distributed computing, exemplar successes, and a strong presence in the emerging world of Linked Open Data are sufficient to conclude that technical issues will be overcome in the foreseeable future. While motivated to have a shared open infrastructure and data pool, and pressured by funding agencies in move in this direction, the sociological issues determine progress. Major sociological issues include our lack of understanding of the heterogeneous data cultures within Life Sciences, and the impediments to progress include a lack of incentives to build appropriate infrastructures into projects and institutions or to encourage scientists to make data openly available. PMID:22207805

  10. Atmospheric propagation issues relevant to optical communications

    NASA Technical Reports Server (NTRS)

    Churnside, James H.; Shaik, Kamran

    1989-01-01

    Atmospheric propagation issues relevant to space-to-ground optical communications for near-earth applications are studied. Propagation effects, current optical communication activities, potential applications, and communication techniques are surveyed. It is concluded that a direct-detection space-to-ground link using redundant receiver sites and temporal encoding is likely to be employed to transmit earth-sensing satellite data to the ground some time in the future. Low-level, long-term studies of link availability, fading statistics, and turbulence climatology are recommended to support this type of application.

  11. Evolution in bioinformatic resources: 2009 update on the Bioinformatics Links Directory.

    PubMed

    Brazas, Michelle D; Yamada, Joseph Tadashi; Ouellette, B F Francis

    2009-07-01

    All of the life science research web servers published in this and previous issues of Nucleic Acids Research, together with other useful tools, databases and resources for bioinformatics and molecular biology research are freely accessible online through the Bioinformatics Links Directory, http://bioinformatics.ca/links_directory/. Entirely dependent on user feedback and community input, the Bioinformatics Links Directory exemplifies an open access research tool and resource. With 112 websites featured in the July 2009 Web Server Issue of Nucleic Acids Research, the 2009 update brings the total number of servers listed in the Bioinformatics Links Directory close to an impressive 1400 links. A complete list of all links listed in this Nucleic Acids Research 2009 Web Server Issue can be accessed online at http://bioinfomatics.ca/links_directory/narweb2009/. The 2009 update of the Bioinformatics Links Directory, which includes the Web Server list and summaries, is also available online at the Nucleic Acids Research website, http://nar.oxfordjournals.org/.

  12. Medical Issues: Breathing

    MedlinePlus

    ... invasive ventilation. For long-term invasive ventilation, a tracheostomy tube is usually required. During a surgical procedure, ... soon as possible after diagnosis. Helpful Links Aaron's Tracheostomy webpage Acute Respiratory Illness Care Guidelines A Young ...

  13. [Comparison of Phoenix™ Yeast ID Panel and API® ID 32C commercial systems for the identification of Candida species isolated from clinical samples].

    PubMed

    Gayibova, Ülkü; Dalyan Cılo, Burcu; Ağca, Harun; Ener, Beyza

    2014-07-01

    Opportunistic fungal pathogens are one of the important causes of nosocomial infections, and several different types of yeasts, especially Candida species are increasingly recovered from immunocompromised patients. Since many of the yeasts are resistant to the commonly used antifungal agents, the introduction of appropriate therapy depends on rapid and accurate identification. The aims of this study were to compare the commercial identification systems namely API® ID 32C (bioMerieux, France) and Phoenix™ Yeast ID Panel (Becton Dickinson Diagnostics, USA) for the identification of Candida species and to evaluate the effect of morphological findings in the identification process. A total of 211 yeast strains isolated from different clinical samples (111 urine, 34 blood/vascular catheter, 27 upper/lower respiratory tract, 16 abscess/pus, 13 throat/vagina swabs and 10 sterile body fluids) of 137 patients hospitalized in Uludag University Health and Research Center between October 2013 to January 2014, were included in the study. Samples were cultured on blood agar, chromogenic agar (CHROMagar Candida, BD, USA) and Saboraud's dextrose agar (SDA), and isolated yeast colonies were evaluated with germ tube test and morphological examination by microscopy on cornmeal/Tween-80 agar. The isolates were identified as well by two commercial systems according to the manufacturers' recommendations. Discrepant results between the systems were tried to be resolved by using morphological characteristics of the yeasts. Of the isolates 159 were identified identical by both of the systems, and the concordance between those systems were estimated as 75.4%. According to the concordant identification, the most frequently isolated species was C.albicans (44.1%) followed by C.tropicalis (9.9%), C.glabrata (9.5%), C.parapsilosis (8.5%) and C.kefyr (8.1%). The concordance rate was 81.7% in identification of frequently isolated species (C.albicans, C.tropicalis, C.parapsilosis, C.glabrata, C

  14. [Comparison of Phoenix™ Yeast ID Panel and API® ID 32C commercial systems for the identification of Candida species isolated from clinical samples].

    PubMed

    Gayibova, Ülkü; Dalyan Cılo, Burcu; Ağca, Harun; Ener, Beyza

    2014-07-01

    Opportunistic fungal pathogens are one of the important causes of nosocomial infections, and several different types of yeasts, especially Candida species are increasingly recovered from immunocompromised patients. Since many of the yeasts are resistant to the commonly used antifungal agents, the introduction of appropriate therapy depends on rapid and accurate identification. The aims of this study were to compare the commercial identification systems namely API® ID 32C (bioMerieux, France) and Phoenix™ Yeast ID Panel (Becton Dickinson Diagnostics, USA) for the identification of Candida species and to evaluate the effect of morphological findings in the identification process. A total of 211 yeast strains isolated from different clinical samples (111 urine, 34 blood/vascular catheter, 27 upper/lower respiratory tract, 16 abscess/pus, 13 throat/vagina swabs and 10 sterile body fluids) of 137 patients hospitalized in Uludag University Health and Research Center between October 2013 to January 2014, were included in the study. Samples were cultured on blood agar, chromogenic agar (CHROMagar Candida, BD, USA) and Saboraud's dextrose agar (SDA), and isolated yeast colonies were evaluated with germ tube test and morphological examination by microscopy on cornmeal/Tween-80 agar. The isolates were identified as well by two commercial systems according to the manufacturers' recommendations. Discrepant results between the systems were tried to be resolved by using morphological characteristics of the yeasts. Of the isolates 159 were identified identical by both of the systems, and the concordance between those systems were estimated as 75.4%. According to the concordant identification, the most frequently isolated species was C.albicans (44.1%) followed by C.tropicalis (9.9%), C.glabrata (9.5%), C.parapsilosis (8.5%) and C.kefyr (8.1%). The concordance rate was 81.7% in identification of frequently isolated species (C.albicans, C.tropicalis, C.parapsilosis, C.glabrata, C

  15. The Coverage Issue

    ERIC Educational Resources Information Center

    Yoshinobu, Stan; Jones, Matthew G.

    2012-01-01

    A significant issue mathematics instructors face is how to cover all the material. Mathematics teachers of all levels have some external and internal pressures to "get through" all the required material. The authors define "the coverage issue" to be the set of difficulties that arise in attempting to cover a lengthy list of topics. Principal among…

  16. Ethical issues in genetics.

    PubMed

    Shannon, T A

    1999-03-01

    The first section of the Notes on Moral Theology reviews ethical issues in genetics through the lenses of privacy-confidentiality; risk-benefit analysis in relation to prenatal diagnosis and gene therapy; and freedom-determinism/human dignity in the context of cloning. The author provides an overview of developments in genetics and highlights thematic issues common to these developments.

  17. Issues in Pupil Transportation.

    ERIC Educational Resources Information Center

    Association of School Business Officials International, Reston, VA.

    The primary purpose of this book is to present the critical issues in pupil transportation that will confront pupil transportation supervisors in local school districts. The following issues are discussed: (1) demands for extended service from community pressure groups; (2) reductions in budget requests by governing bodies; (3) unrest among driver…

  18. Issue Brief on Diversity

    ERIC Educational Resources Information Center

    Division on Developmental Disabilities, Council for Exceptional Children (NJ1), 2013

    2013-01-01

    During the past year, the Diversity Committee of the Division of Developmental Disabilities (DDD) Board worked with the Board and the Issues Committee Chair to develop an issue brief addressing diversity, its impact on the membership and the wider community that is served by the work of DDD, resulting in recommendations that will influence policy…

  19. ISSUES IN AMERICAN EDUCATION.

    ERIC Educational Resources Information Center

    WEINBERG, MEYER

    THIS ARTICLE DISCUSSES THREE PROMINENT ISSUES OF CONTEMPORARY AMERICAN EDUCATION--UNEQUAL LEARNING OPPORTUNITIES, ACADEMIC FREEDOM, AND THE TEACHER AS A PROFESSIONAL WORKER. THE FIRST OF THESE ISSUES IS EXAMINED UNDER THE RUBRICS OF (1) THE GOALS OF INTEGRATION, (2) THE SEPARATION OF EDUCATION FROM INTEGRATION, (3) COMPENSATORY EDUCATION, (4)…

  20. Legal Issues in Testing.

    ERIC Educational Resources Information Center

    Childs, Ruth Axman

    Court cases are presented that illustrate some of the issues that courts have dealt with in the area of educational testing. While this study was developed primarily for parents, the booklet should also help school administrators and testing professionals learn about court cases and precedents relevant to some major issues in educational testing.…

  1. TRICHLOROETHYLENE (TCE) ISSUE PAPERS

    EPA Science Inventory

    These issue papers are a part of EPA's effort to develop a trichloroethylene (TCE) human health risk assessment. These issue papers were developed by EPA to provide scientific and technical information to the National Academy of Sciences (NAS) for use in developing their advice ...

  2. News and Issues, 2002.

    ERIC Educational Resources Information Center

    Oshinsky, Carole J., Ed.

    2002-01-01

    This document is comprised of the three 2002 issues of a newsletter devoted to identifying and promoting strategies to reduce the poverty rate of young children and to improve the life chances of children still living in poverty. The Winter issue includes the following articles: (1) "NCCP [National Center for Children in Poverty] Responds to New…

  3. BWR internal cracking issues

    SciTech Connect

    Carpenter, C.E. Jr.; Lund, A.L.

    1999-07-01

    The regulatory issues associated with cracking of boiling water reactor (BWR) internals is being addressed by the Nuclear Regulatory Commission (NRC) staff and is the subject of a voluntary industry initiative. The lessons learned from this effort will be applied to pressurized water reactor (PWR) internals cracking issues.

  4. Navigating "Thorny" Issues

    ERIC Educational Resources Information Center

    Hutchinson, Kashema; Gilbert, Aderinsola; Malyukova, Anna

    2016-01-01

    In their article "Mindfulness and discussing 'thorny' issues in the classroom" Konstantinos Alexakos et al. ("Cult Stud Sci Educ," 2016. doi: 10.1007/s11422-015-9718-0) describe "thorny" issues as "difficult topics to discuss because they are more personal to some perhaps even cause pain and violence." As…

  5. Preface: ISBB Special Issue

    Technology Transfer Automated Retrieval System (TEKTRAN)

    This special issue is published for the International Society of Biocatalysis and Biotechnology (ISBB). The ISBB special issue is devoted to all areas of biocatalysis and agricultural biotechnology in which biological systems are developed and/or used for the provision of commercial goods or serv...

  6. The ESO Telescope Bibliography on the Web: Linking Publications and Observations

    NASA Astrophysics Data System (ADS)

    Grothkopf, U.; Delmotte, N.; Leibundgut, B.; Rainer, N.; Treumann, A.

    2004-12-01

    We present a web interface that allows users to keep track of the entire lifetime of VLT (Very Large Telescope) programs: from proposals to observations and publications. It links scientific papers with the observing proposals and archival data, located at the ESO/ST-ECF Science Archive Facility. This service is a prerequisite to the astronomical Virtual Observatory. The interface provides search options by bibliographic details (author, title, journal, publication year etc.) as well as by observatory site, telescope, instrument and program ID. Papers based on ESO observations can also be retrieved through the ADS Abstract Service from where active links to the ESO Archive are provided.

  7. 1-Hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate reductase (IDS) is encoded by multicopy genes in gymnosperms Ginkgo biloba and Pinus taeda.

    PubMed

    Kim, Sang-Min; Kuzuyama, Tomohisa; Kobayashi, Akio; Sando, Tomoki; Chang, Yung-Jin; Kim, Soo-Un

    2008-01-01

    Isoprenoids are synthesized through the condensation of five-carbon intermediates, isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP), derived from two distinct biosynthetic routes: cytosolic mevalonate (MVA) and plastidial 2-C-methyl-D: -erythritol 4-phosphate (MEP) pathways. 1-Hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate reductase (IDS; EC 1.17.1.2), which catalyzes the last step of MEP pathway, was cloned as a multicopy gene from gymnosperms Ginkgo biloba (GbIDS1, GbIDS2, and GbIDS2-1) and Pinus taeda (PtIDS1 and PtIDS2), and characterized. Phylogenetic tree constructed with other plant IDSs demonstrated gymnosperm IDSs were distinctively different from angiosperm IDSs. The gymnosperm IDS clade contained two subclades, one composed of GbIDS1 and PtIDS1, and the other composed of GbIDS2, GbIDS2-1, and PtIDS2. G. biloba IDSs, except GbIDS2-1, successfully complemented Escherichia coli DLYT1, a lytB disruptant, confirming the in vivo competency of isozymes. During the 4 weeks study period, although transcript levels of GbIDS1s were similar both in roots and leaves of cultured G. biloba embryo, the transcripts of GbIDS2 predominantly occurred in the embryo roots, where diterpene ginkgolides are biosynthesized. Levels of PtIDS2 transcripts in the diterpenoid resin-producing wood were 4-5 times higher than those in other tissues. Higher levels of GbIDS1 transcripts were induced by light, whereas those of GbIDS2 were increased by methyl jasmonate treatment. These results strongly imply GbIDS2 and PtIDS2 have high correlation with secondary metabolism. In Arabidopsis transient expression system, N-terminal 100 amino acid residues of GbIDS1 delivered fused GFP protein into chloroplast as well as cytosol and nucleus, whereas those of GbIDS2, GbIDS2-1, and two PtIDSs delivered GFP only into chloroplast.

  8. CERES Web Links

    Atmospheric Science Data Center

    2013-03-21

        Web Links to Relevant CERES Information Relevant information about ... for Satellite Applications and Research) Surface and Atmospheric Radiation Budget (SARB) Working Group homepage ... information for CRS users including: CERES Surface Properties . CERES Ocean Validation Experiment (COVE)  at ...

  9. University-linked localities

    PubMed Central

    Spicer, John; Gnani, Shamini

    2012-01-01

    In this article, we propose that reframing the old concept of ‘academic general practices’ as ‘university-linked localities’ will help to integrate the work of those leading commissioning, education, research and public health. It will provide a ‘playground’ for different disciplines to creatively interact for the benefit of all. PMID:26265944

  10. Helically linked mirror arrangement

    SciTech Connect

    Ranjan, P.

    1986-08-01

    A scheme is described for helical linking of mirror sections, which endeavors to combine the better features of toroidal and mirror devices by eliminating the longitudinal loss of mirror machines, having moderately high average ..beta.. and steady state operation. This scheme is aimed at a device, with closed magnetic surfaces having rotational transform for equilibrium, one or more axisymmetric straight sections for reduced radial loss, a simple geometrical axis for the links and an overall positive magnetic well depth for stability. We start by describing several other attempts at linking of mirror sections, made both in the past and the present. Then a description of our helically linked mirror scheme is given. This example has three identical straight sections connected by three sections having helical geometric axes. A theoretical analysis of the magnetic field and single-particle orbits in them leads to the conclusion that most of the passing particles would be confined in the device and they would have orbits independent of pitch angle under certain conditions. Numerical results are presented, which agree well with the theoretical results as far as passing particle orbits are concerned.

  11. Linking Literacy and Movement

    ERIC Educational Resources Information Center

    Pica, Rae

    2010-01-01

    There are many links between literacy and movement. Movement and language are both forms of communication and self-expression. Rhythm is an essential component of both language and movement. While people may think of rhythm primarily in musical terms, there is a rhythm to words and sentences as well. Individuals develop an internal rhythm when…

  12. Navigating "thorny" issues

    NASA Astrophysics Data System (ADS)

    Hutchinson, Kashema; Gilbert, Aderinsola; Malyukova, Anna

    2016-09-01

    In their article Mindfulness and discussing ` thorny' issues in the classroom Konstantinos Alexakos et al. (Cult Stud Sci Educ, 2016. doi: 10.1007/s11422-015-9718-0) describe "thorny" issues as "difficult topics to discuss because they are more personal to some perhaps even cause pain and violence." As women from different backgrounds, we engage in a metalogue, which expands on our thoughts and emotions the thorny issues evoked. Our discussion is grounded in theoretical frameworks of mindfulness, wellness, and safe space for learning. We also reflect on our experiences of facing some of the thorny issues in our academic and personal lives. Having trust and respect for one another and being aware of thorny issues allows for a meaningful conversation about the complexity and nuances involved in discussing difficult topics in a classroom setting.

  13. Navigating "thorny" issues

    NASA Astrophysics Data System (ADS)

    Hutchinson, Kashema; Gilbert, Aderinsola; Malyukova, Anna

    2016-08-01

    In their article Mindfulness and discussing `thorny' issues in the classroom Konstantinos Alexakos et al. (Cult Stud Sci Educ, 2016. doi: 10.1007/s11422-015-9718-0) describe "thorny" issues as "difficult topics to discuss because they are more personal to some perhaps even cause pain and violence." As women from different backgrounds, we engage in a metalogue, which expands on our thoughts and emotions the thorny issues evoked. Our discussion is grounded in theoretical frameworks of mindfulness, wellness, and safe space for learning. We also reflect on our experiences of facing some of the thorny issues in our academic and personal lives. Having trust and respect for one another and being aware of thorny issues allows for a meaningful conversation about the complexity and nuances involved in discussing difficult topics in a classroom setting.

  14. Current issues on epileptic women.

    PubMed

    Pimentel, J

    2000-05-01

    Issues linked to epileptic women are being reviewed. Ovarian steroid hormones have a number of effects on the brain that predispose to epileptic activity. In particular, estradiol produces changes in the hippocampus synapses predisposing hyperexcitability associated with seizures. Also, menses and menopause periods, in which there are changing levels of steroid ovarian hormones, are associated with a particular appearing of seizures (catamenial epilepsy) and with phenotypic changes of previous ones. Epilepsy can affect the reproductive system, inducing endocrinal abnormalities (through disruption of cortical regulation of hypothalamus hormone release, and changes in the central nervous system concentration of steroid hormones induced by antiepileptics), infertility (linked to abnormalities in menstrual cycle or to the occurrence of polycytic ovaries, particularly in association with valproate treatment), and sexual disfunction (namely related to physiologic defects). Oral hormonal contraceptives should be performed using a pill with > 50mg of estrogen in order to prevent its potential loss of efficacy induced by enzyme-inducing antiepileptics. Concerning pregnancy, some topics should be discussed with, and advised to epileptic women, including: the possibility of withdrawal antiepileptics and the need of folic acid supplementation when planning a pregnancy; the risk of increased seizure frequency during pregnancy, and of the occurrence of obstetric complications; the increased risk of teratogenesis associated with antiepileptic therapy (mainly if in polytherapy); the need of vitamin K supplementation during the last month of pregnancy in order to avoid newborn haemorrhages; and the general absence of risk of breastfeeding even under sustained antiepileptic therapy. PMID:10828312

  15. Site mitigation issues along the Alameda Corridor

    SciTech Connect

    Ripaldi, C.P.

    1996-12-31

    The Alameda Corridor is a consolidated railroad link between the Ports of Los Angeles and Long Beach and the regional and national rail systems linking the nation. A joint Environmental Impacts Report/Environmental Impact Statement (EIR/EIS) was prepared for the project. The Final EIS was issued in February 1996, and a record of decision was issued in May, 1996. Various Phase 1 and Phase 2 Environmental Site Assessments have provided extensive historical documentation of environmental contamination in the vicinity of the Alameda Corridor Transportation Project. A Site Mitigation Master Plan provides guidance and direction for the clean-up activities. Samples will be analyzed for metals, PCB`s TRPH, BTEX, and VOCs.

  16. Advanced Q-switched DPSS lasers for ID-card marking

    NASA Astrophysics Data System (ADS)

    Hertwig, Michael; Paster, Martin; Terbrueggen, Ralf

    2008-02-01

    Increased homeland security concerns across the world have generated a strong demand for forgery-proof ID documents. Manufacturers currently employ a variety of high technology techniques to produce documents that are difficult to copy. However, production costs and lead times are still a concern when considering any possible manufacturing technology. Laser marking has already emerged as an important tool in the manufacturer's arsenal, and is currently being utilized to produce a variety of documents, such as plastic ID cards, drivers' licenses, health insurance cards and passports. The marks utilized can range from simple barcodes and text to high resolution, true grayscale images. The technical challenges posed by these marking tasks include delivering adequate mark legibility, minimizing substrate burning or charring, accurately reproducing grayscale data, and supporting the required process throughput. This article covers the advantages and basic requirements on laser marking of cards and reviews how laser output parameters affect marking quality, speed and overall process economics.

  17. 2. Freud's 'id' and Jung's 'self' as aids in self-analysis.

    PubMed

    Plaut, Alfred B J

    2005-02-01

    The id and the self are described as constructs of our unconscious which can be deployed in describing the analytic process. They can be used like the x in mathematics or the joker in a pack of cards as fitting in almost anywhere. But we can call on them as important aids to concentration, when additional room is made for meditating on the past which then comes to life again. They are useful for analysing ourselves and others. In this way the id as well as the self become aide-mémoires. The analysis of the repressed by means of the transference is not the only road to hitherto unconscious memories. This kind of meditation has helped me despite all reservations to undertake self-analysis and to write an autobiography. I maintain that one can have a transference to oneself. It is, of course, narcissistic. But then, no one can write an autobiography without a healthy dose of narcissism.

  18. ROLE OF LEUCONOSTOC MESENTEROIDES IN LEAVENING THE BATTER OF IDLI, A FERMENTED FOOD OF INDIA.

    PubMed

    MUKHERJEE, S K; ALBURY, M N; PEDERSON, C S; VANVEEN, A G; STEINKRAUS, K H

    1965-03-01

    The fermentation of the batter of idli, a fermented food of India, was studied. The microorganisms responsible for the characteristic changes in the batter were isolated and identified. Although there is a sequential change in the bacterial flora, the predominant microorganism responsible for souring, as well as for gas production, was found to be Leuconostoc mesenteroides. In the later stages of fermentation, growth of Streptococcus faecalis and, still later, of Pediococcus cerevisiae becomes significant. The fermentation of idli demonstrates a leavening action caused by the activity of the heterofermentative lactic acid bacterium, L. mesenteroides. As far as is known, this is the first record of a leavening action produced exclusively by the activity of a lactic acid bacterium.

  19. ROLE OF LEUCONOSTOC MESENTEROIDES IN LEAVENING THE BATTER OF IDLI, A FERMENTED FOOD OF INDIA.

    PubMed

    MUKHERJEE, S K; ALBURY, M N; PEDERSON, C S; VANVEEN, A G; STEINKRAUS, K H

    1965-03-01

    The fermentation of the batter of idli, a fermented food of India, was studied. The microorganisms responsible for the characteristic changes in the batter were isolated and identified. Although there is a sequential change in the bacterial flora, the predominant microorganism responsible for souring, as well as for gas production, was found to be Leuconostoc mesenteroides. In the later stages of fermentation, growth of Streptococcus faecalis and, still later, of Pediococcus cerevisiae becomes significant. The fermentation of idli demonstrates a leavening action caused by the activity of the heterofermentative lactic acid bacterium, L. mesenteroides. As far as is known, this is the first record of a leavening action produced exclusively by the activity of a lactic acid bacterium. PMID:14325884

  20. Role of Leuconostoc mesenteroides in Leavening the Batter of Idli, a Fermented Food of India1

    PubMed Central

    Mukherjee, S. K.; Albury, M. N.; Pederson, C. S.; Van Veen, A. G.; Steinkraus, K. H.

    1965-01-01

    The fermentation of the batter of idli, a fermented food of India, was studied. The microorganisms responsible for the characteristic changes in the batter were isolated and identified. Although there is a sequential change in the bacterial flora, the predominant microorganism responsible for souring, as well as for gas production, was found to be Leuconostoc mesenteroides. In the later stages of fermentation, growth of Streptococcus faecalis and, still later, of Pediococcus cerevisiae becomes significant. The fermentation of idli demonstrates a leavening action caused by the activity of the heterofermentative lactic acid bacterium, L. mesenteroides. As far as is known, this is the first record of a leavening action produced exclusively by the activity of a lactic acid bacterium. Images Fig. 3 PMID:14325884

  1. Temperament: concepts, issues and problems.

    PubMed

    Rutter, M

    1982-01-01

    There are marked individual differences in children's temperamental styles-- differences thought to be constitutionally determined in part. The importance of temperamental features is evident in their links with various forms of psychopathology and in their effects on the manner in which other people respond to the child. For these and other reasons it has rightly come to be accepted that greater attention needs to be paid to temperamental issues in consideration of the processes of development, children's responses to stress situations, and the genesis of emotional, behavioural and learning disorders. However, major conceptual, methodological and theoretical problems remain. Problems of measurement are considered in terms of the relativity of measures, whether or not to take social context into account, the functional equivalence of measures at different ages, the circumstances to use in assessing temperament, the choice of measuring instrument and the categorization of temperamental features. The issues involved in the meaning of temperamental differences are discussed with respect to consistency, developmental change, genetic influences, brain damage and mental retardation, sex differences and the mechanisms by which temperamental variables exert their effects.

  2. Synaptic and extrasynaptic traces of long-term memory: the ID molecule theory.

    PubMed

    Legéndy, Charles R

    2016-08-01

    It is generally assumed at the time of this writing that memories are stored in the form of synaptic weights. However, it is now also clear that the synapses are not permanent; in fact, synaptic patterns undergo significant change in a matter of hours. This means that to implement the long survival of distant memories (for several decades in humans), the brain must possess a molecular backup mechanism in some form, complete with provisions for the storage and retrieval of information. It is found below that the memory-supporting molecules need not contain a detailed description of mental entities, as had been envisioned in the 'memory molecule papers' from 50 years ago, they only need to contain unique identifiers of various entities, and that this can be achieved using relatively small molecules, using a random code ('ID molecules'). In this paper, the logistics of information flow are followed through the steps of storage and retrieval, and the conclusion reached is that the ID molecules, by carrying a sufficient amount of information (entropy), can effectively control the recreation of complex multineuronal patterns. In illustrations, it is described how ID molecules can be made to revive a selected cell assembly by waking up its synapses and how they cause a selected cell assembly to ignite by sending slow inward currents into its cells. The arrangement involves producing multiple copies of the ID molecules and distributing them at strategic locations at selected sets of synapses, then reaching them through small noncoding RNA molecules. This requires the quick creation of entropy-rich messengers and matching receptors, and it suggests that these are created from each other by small-scale transcription and reverse transcription. PMID:27206318

  3. Fine wavelength id for tunable laser local oscillators. [sensing the absorption emission spectra of atmospheric gases

    NASA Technical Reports Server (NTRS)

    Savage, M. G.; Augeri, R. C.

    1980-01-01

    A wavelength ID device which consists of an electronic show that the etalon has a finesse F 30 which is maintainable for several days. These tests also demonstrate that the etalon system is capable of resonance frequency stability during similar time periods. With currently available coatings, this level of performance is achievable over an optical bandwidth delta lambda = 3 micrometers centered at lambda = 10 micrometers.

  4. Preparation of idli batter, its properties and nutritional improvement during fermentation.

    PubMed

    Ghosh, Debasree; Chattopadhyay, Parimal

    2011-10-01

    Idli is a traditional fermented rice and black gram based food. Idli batter is prepared by soaking polished parboiled rice and decorticated black gram for 4 h at 30 ± 1 °C in water. The soaked mass was ground using a grinder with adequate amount of water. The blend ratios of 2:1, 3:1 and 4:1 (w/w) batter were allowed for fermentation for different periods with the addition of 2% (w/w) of salt. The rheology of the product was assessed using a Brookfield Viscometer having disc spindles. Shear stress values were in the range of 0.22 and 4 Pa and reached a maximum value at 7 h of fermentation. The density, pH, and percentage total acidity of batter during fermentation for different blend ratios ranged between 0.93 and 0.59 gm cm(-3), 4.21 and 5.9 and 0.44 and 0.91% respectively. During fermentation, maximum production of riboflavin and thiamine were found to be 0.76 mg/100 gm and 0.73 mg/100 gm in 3:1 blend ratio of idli batter; and the folic acid content was found to be at a maximum of 0.75 mg/100 gm of idli batter after 10 h of fermentation. Digestibility in terms of amino N2 content was analysed by formol titration.

  5. Evaluation of the Organon-Teknika MICRO-ID LISTERIA system.

    PubMed

    Bannerman, E; Yersin, M N; Bille, J

    1992-06-01

    The MICRO-ID LISTERIA system, designed to identify Listeria isolates to species level within 24 h, was compared with conventional biochemical identification. MICRO-ID LISTERIA used in combination with the CAMP test correctly identified 409 (98.8%) of 414 strains isolated from human, animal, food, and environmental sources belonging to the seven species currently defined within the genus Listeria. The kit was easy to use and simple to interpret. However, 8 of the 15 tests (i.e., phenylalanine deaminase, hydrogen sulfide, indole, ornithine decarboxylase, lysine decarboxylase, malonate, urease, and o-nitrophenyl-beta-D-galactopyranoside) were considered superfluous for the differentiation of Listeria spp. The CAMP test was indispensable when using the MICRO-ID LISTERIA system, in particular to differentiate CAMP test-positive L. monocytogenes from the nonhemolytic, rhamnose-positive L. innocua. The hemolytic L. seeligeri and L. ivanovii strains and the nonhemolytic, non-rhamnose-acidifying L. welshimeri strains could also be differentiated from one another only on the basis of their CAMP test results. The very few strains of L. grayi and L. murrayi were easily differentiated from the other nonhemolytic species. Catalase-negative cocci should not be tested, because 12 out of 19 catalase-negative strains (all enterococci) in our test were misidentified as Listeria spp. The MICRO-ID LISTERIA system identified strains within 18 to 24 h and is thus less time-consuming than conventional tests. The system could, therefore, be used together with correctly done CAMP tests for the rapid identification of Listeria isolates, especially food and environmental isolates, for which rapid species differentiation is important.

  6. Evaluation of the Organon-Teknika MICRO-ID LISTERIA system.

    PubMed Central

    Bannerman, E; Yersin, M N; Bille, J

    1992-01-01

    The MICRO-ID LISTERIA system, designed to identify Listeria isolates to species level within 24 h, was compared with conventional biochemical identification. MICRO-ID LISTERIA used in combination with the CAMP test correctly identified 409 (98.8%) of 414 strains isolated from human, animal, food, and environmental sources belonging to the seven species currently defined within the genus Listeria. The kit was easy to use and simple to interpret. However, 8 of the 15 tests (i.e., phenylalanine deaminase, hydrogen sulfide, indole, ornithine decarboxylase, lysine decarboxylase, malonate, urease, and o-nitrophenyl-beta-D-galactopyranoside) were considered superfluous for the differentiation of Listeria spp. The CAMP test was indispensable when using the MICRO-ID LISTERIA system, in particular to differentiate CAMP test-positive L. monocytogenes from the nonhemolytic, rhamnose-positive L. innocua. The hemolytic L. seeligeri and L. ivanovii strains and the nonhemolytic, non-rhamnose-acidifying L. welshimeri strains could also be differentiated from one another only on the basis of their CAMP test results. The very few strains of L. grayi and L. murrayi were easily differentiated from the other nonhemolytic species. Catalase-negative cocci should not be tested, because 12 out of 19 catalase-negative strains (all enterococci) in our test were misidentified as Listeria spp. The MICRO-ID LISTERIA system identified strains within 18 to 24 h and is thus less time-consuming than conventional tests. The system could, therefore, be used together with correctly done CAMP tests for the rapid identification of Listeria isolates, especially food and environmental isolates, for which rapid species differentiation is important. PMID:1622280

  7. A non-parametric peak calling algorithm for DamID-Seq.

    PubMed

    Li, Renhua; Hempel, Leonie U; Jiang, Tingbo

    2015-01-01

    Protein-DNA interactions play a significant role in gene regulation and expression. In order to identify transcription factor binding sites (TFBS) of double sex (DSX)-an important transcription factor in sex determination, we applied the DNA adenine methylation identification (DamID) technology to the fat body tissue of Drosophila, followed by deep sequencing (DamID-Seq). One feature of DamID-Seq data is that induced adenine methylation signals are not assured to be symmetrically distributed at TFBS, which renders the existing peak calling algorithms for ChIP-Seq, including SPP and MACS, inappropriate for DamID-Seq data. This challenged us to develop a new algorithm for peak calling. A challenge in peaking calling based on sequence data is estimating the averaged behavior of background signals. We applied a bootstrap resampling method to short sequence reads in the control (Dam only). After data quality check and mapping reads to a reference genome, the peaking calling procedure compromises the following steps: 1) reads resampling; 2) reads scaling (normalization) and computing signal-to-noise fold changes; 3) filtering; 4) Calling peaks based on a statistically significant threshold. This is a non-parametric method for peak calling (NPPC). We also used irreproducible discovery rate (IDR) analysis, as well as ChIP-Seq data to compare the peaks called by the NPPC. We identified approximately 6,000 peaks for DSX, which point to 1,225 genes related to the fat body tissue difference between female and male Drosophila. Statistical evidence from IDR analysis indicated that these peaks are reproducible across biological replicates. In addition, these peaks are comparable to those identified by use of ChIP-Seq on S2 cells, in terms of peak number, location, and peaks width.

  8. A non-parametric peak calling algorithm for DamID-Seq.

    PubMed

    Li, Renhua; Hempel, Leonie U; Jiang, Tingbo

    2015-01-01

    Protein-DNA interactions play a significant role in gene regulation and expression. In order to identify transcription factor binding sites (TFBS) of double sex (DSX)-an important transcription factor in sex determination, we applied the DNA adenine methylation identification (DamID) technology to the fat body tissue of Drosophila, followed by deep sequencing (DamID-Seq). One feature of DamID-Seq data is that induced adenine methylation signals are not assured to be symmetrically distributed at TFBS, which renders the existing peak calling algorithms for ChIP-Seq, including SPP and MACS, inappropriate for DamID-Seq data. This challenged us to develop a new algorithm for peak calling. A challenge in peaking calling based on sequence data is estimating the averaged behavior of background signals. We applied a bootstrap resampling method to short sequence reads in the control (Dam only). After data quality check and mapping reads to a reference genome, the peaking calling procedure compromises the following steps: 1) reads resampling; 2) reads scaling (normalization) and computing signal-to-noise fold changes; 3) filtering; 4) Calling peaks based on a statistically significant threshold. This is a non-parametric method for peak calling (NPPC). We also used irreproducible discovery rate (IDR) analysis, as well as ChIP-Seq data to compare the peaks called by the NPPC. We identified approximately 6,000 peaks for DSX, which point to 1,225 genes related to the fat body tissue difference between female and male Drosophila. Statistical evidence from IDR analysis indicated that these peaks are reproducible across biological replicates. In addition, these peaks are comparable to those identified by use of ChIP-Seq on S2 cells, in terms of peak number, location, and peaks width. PMID:25785608

  9. Preparation of idli batter, its properties and nutritional improvement during fermentation.

    PubMed

    Ghosh, Debasree; Chattopadhyay, Parimal

    2011-10-01

    Idli is a traditional fermented rice and black gram based food. Idli batter is prepared by soaking polished parboiled rice and decorticated black gram for 4 h at 30 ± 1 °C in water. The soaked mass was ground using a grinder with adequate amount of water. The blend ratios of 2:1, 3:1 and 4:1 (w/w) batter were allowed for fermentation for different periods with the addition of 2% (w/w) of salt. The rheology of the product was assessed using a Brookfield Viscometer having disc spindles. Shear stress values were in the range of 0.22 and 4 Pa and reached a maximum value at 7 h of fermentation. The density, pH, and percentage total acidity of batter during fermentation for different blend ratios ranged between 0.93 and 0.59 gm cm(-3), 4.21 and 5.9 and 0.44 and 0.91% respectively. During fermentation, maximum production of riboflavin and thiamine were found to be 0.76 mg/100 gm and 0.73 mg/100 gm in 3:1 blend ratio of idli batter; and the folic acid content was found to be at a maximum of 0.75 mg/100 gm of idli batter after 10 h of fermentation. Digestibility in terms of amino N2 content was analysed by formol titration. PMID:23572795

  10. Issues management made easier

    SciTech Connect

    Brownson, L.

    1993-10-01

    Increases in ES&H compliance issues within the past few years have necessitated a formal process by which DOE facilities address these issues. In May 1991, ANL-W implemented the ANL-W Issues Management System (IMS) to facilitate the management of compliance issues and scheduling of corrective action plans with limited resources. The central focus of this process is a computer database, Integrated Resource Management System (IRMS), which allows quick retrieval of compliance information, organization of compliance issues based on a risk-based prioritization methodology, and tracking of corrective action plans. Without the IRMS, the ANL-W Issues Management System would have been difficult to administer and manage. ANL-W has used the IRMS for both audit preparation and audit response, most noticeably the preparation and subsequent response to the 1991 Tiger Team audit. The IRMS was used to track ANL-W Self-Assessment corrective action plans, provide instant information to Tiger Team members regarding Self-Assessment findings, produce prioritized lists of Tiger Team concerns for developing corrective action plans, and track Tiger Team corrective action plans. Status reports to senior, laboratory management regarding the Tiger Team corrective action plan are produced based on information provided by the IRMS. This paper discusses the criteria used for selecting the IRMS, implementation of the Issues Management System using the IRMS, lessons learned, and the future evolution of the IRMS.

  11. Optical Ethernet: introduction to the feature issue

    NASA Astrophysics Data System (ADS)

    Lam, Cedric F.; Tsang, Danny H. K.

    2002-08-01

    Ethernet and IP (Internet Protocol) have become the ubiquitous protocols for today's data communication networks, with Ethernet being used to provide the physical layer and data-link layer functions and IP for routing and internetworking in the network layer. Here feature editors Cedric F. Lam and Danny H. K. Tsang discuss the emerging technology of Optical Ethernet and introduce the papers in this feature issue of JON.

  12. Expression of Id proteins is regulated by the Bcl-3 proto-oncogene in prostate cancer.

    PubMed

    Ahlqvist, K; Saamarthy, K; Syed Khaja, A S; Bjartell, A; Massoumi, R

    2013-03-21

    B-cell leukemia 3 (Bcl-3) is a member of the inhibitor of κB family, which regulates a wide range of biological processes by functioning as a transcriptional activator or as a repressor of target genes. As high levels of Bcl-3 expression and activation have been detected in different types of human cancer, Bcl-3 has been labeled a proto-oncogene. Our study uncovered a markedly upregulated Bcl-3 expression in human prostate cancer (PCa), where inflammatory cell infiltration was observed. Elevated Bcl-3 expression in PCa was dependent on the proinflammatory cytokine interleukin-6-mediated STAT3 activation. Microarray analyses, using Bcl-3 knockdown in PCa cells, identified the inhibitor of DNA-binding (Id) family of helix-loop-helix proteins as potential Bcl-3-regulated genes. Bcl-3 knockdown reduced the abundance of Id-1 and Id-2 proteins and boosted PCa cells to be more receptive to undergoing apoptosis following treatment with anticancer drug. Our data imply that inactivation of Bcl-3 may lead to sensitization of cancer cells to chemotherapeutic drug-induced apoptosis, thus suggesting a potential therapeutic strategy in PCa treatment.

  13. RNA-ID, a Powerful Tool for Identifying and Characterizing Regulatory Sequences.

    PubMed

    Brule, C E; Dean, K M; Grayhack, E J

    2016-01-01

    The identification and analysis of sequences that regulate gene expression is critical because regulated gene expression underlies biology. RNA-ID is an efficient and sensitive method to discover and investigate regulatory sequences in the yeast Saccharomyces cerevisiae, using fluorescence-based assays to detect green fluorescent protein (GFP) relative to a red fluorescent protein (RFP) control in individual cells. Putative regulatory sequences can be inserted either in-frame or upstream of a superfolder GFP fusion protein whose expression, like that of RFP, is driven by the bidirectional GAL1,10 promoter. In this chapter, we describe the methodology to identify and study cis-regulatory sequences in the RNA-ID system, explaining features and variations of the RNA-ID reporter, as well as some applications of this system. We describe in detail the methods to analyze a single regulatory sequence, from construction of a single GFP variant to assay of variants by flow cytometry, as well as modifications required to screen libraries of different strains simultaneously. We also describe subsequent analyses of regulatory sequences.

  14. Purification, crystallization and preliminary crystallographic study of an IDS-epimerase from Agrobacterium tumefaciens BY6

    SciTech Connect

    Bäuerle, Bettina; Sandalova, Tatyana; Schneider, Gunter; Rieger, Paul-Gerhard

    2006-08-01

    This is the first report of the crystallization of an IDS-epimerase from A. tumefaciens BY6 and its l-selenomethionine derivative. The initial degradation of all stereoisomers of the complexing agent iminodisuccinate (IDS) is enabled by an epimerase in the bacterial strain Agrobacterium tumefaciens BY6. This protein was produced in Escherichia coli, purified and crystallized by the hanging-drop vapour-diffusion method. Crystals of IDS-epimerase were obtained under several conditions. The best diffracting crystals were grown in 22% PEG 3350, 0.2 M (NH{sub 4}){sub 2}SO{sub 4} and 0.1 M bis-Tris propane pH 7.2 at 293 K. These crystals belong to the monoclinic space group P2{sub 1}, with unit-cell parameters a = 55.4, b = 104.2, c = 78.6 Å, β = 103.3°, and diffracted to 1.7 Å resolution. They contain two protein molecules per asymmetric unit. In order to solve the structure using the MAD phasing method, crystals of the l-selenomethionine-substituted epimerase were grown in the presence of 20% PEG 3350, 0.2 M Na{sub 2}SO{sub 4} and 0.1 M bis-Tris propane pH 8.5.

  15. Towards fraud-proof ID documents using multiple data hiding technologies and biometrics

    NASA Astrophysics Data System (ADS)

    Picard, Justin; Vielhauer, Claus; Thorwirth, Niels

    2004-06-01

    Identity documents, such as ID cards, passports, and driver's licenses, contain textual information, a portrait of the legitimate holder, and eventually some other biometric characteristics such as a fingerprint or handwritten signature. As prices for digital imaging technologies fall, making them more widely available, we have seen an exponential increase in the ease and the number of counterfeiters that can effectively forge documents. Today, with only limited knowledge of technology and a small amount of money, a counterfeiter can effortlessly replace a photo or modify identity information on a legitimate document to the extent that it is very diffcult to differentiate from the original. This paper proposes a virtually fraud-proof ID document based on a combination of three different data hiding technologies: digital watermarking, 2-D bar codes, and Copy Detection Pattern, plus additional biometric protection. As will be shown, that combination of data hiding technologies protects the document against any forgery, in principle without any requirement for other security features. To prevent a genuine document to be used by an illegitimate user,biometric information is also covertly stored in the ID document, to be used for identification at the detector.

  16. Validating crustal velocities derived from the IDS contribution to ITRF201

    NASA Astrophysics Data System (ADS)

    Moreaux, G.; Soudarin, L.; Gravelle, M.; Santamaria Gomez, A.; Lemoine, F. G.; Willis, P.; Ferrage, P.; Argus, D. F.

    2015-12-01

    In the context of the ITRF2014, the IDS Combination Center delivered 1140 weekly SINEX files to the IERS containing DORIS station positions and Earth orientation parameters from January 1993 to December 2014 (series IDS 09). For each DORIS station, we computed the mean position and velocity by stacking the IDS 09 weekly solutions and combining them with both DORIS internal local ties (3-D vector between two successive beacon installations at the same DORIS site) from IGN and a discontinuity file. The first objective of this study is to compare DORIS horizontal velocities to estimations from tectonic models such as GEODVEL and NNR-MORVEL56. The second purpose is to evaluate DORIS vertical velocities with respect to Global Isostatic Adjustment models (for Greenland, Fennoscandia, Iceland and Antarctica sites) such as ICE-6G_C, and with respect to the latests GPS solution at the University of La Rochelle. Sites showing large differences in either horizontal (ex: Arequipa, Dionysos/Gavdos, Goldstone, Manila and Santiago) or vertical velocities (ex: Rothera, Thule and Yellowknife) will be of special concern.

  17. The International DORIS Service (IDS) - Recent Developments in Preparation for ITRF2013

    NASA Technical Reports Server (NTRS)

    Willis, Pascal; Lemoine, Frank G.; Moreaux, Guilhem; Soudarin, Laurent; Ferrage, Pascale; Ries, John; Otten, Michiel; Saunier, Jerome; Noll, Carey E.; Biancale, Richard; Luzum, Brian

    2014-01-01

    The International DORIS Service (IDS) was created in 2003 under the umbrella of the International Association of Geodesy (IAG) to foster scientific research related to the French DORIS tracking system and to deliver scientific products, mostly related to the International Earth rotation and Reference systems Service (IERS). We first present some general background related to the DORIS system (current and planned satellites, current tracking network and expected evolution) and to the general IDS organization (from Data Centers, Analysis Centers and Combination Center). Then, we discuss some of the steps recently taken to prepare the IDS submission to ITRF2013 (combined weekly time series based on individual solutions from several Analysis Centers). In particular, recent results obtained from the Analysis Centers and the Combination Center show that improvements can still be made when updating physical models of some DORIS satellites, such as Envisat, Cryosat-2 or Jason-2. The DORIS contribution to ITRF2013 should also benefit from the larger number of ground observations collected by the last generation of DGXX receivers (first instrument being onboard Jason-2 satellite). In particular for polar motion, sub-millarcsecond accuracy seems now to be achievable. Weekly station positioning internal consistency also seems to be improved with a larger DORIS constellation.

  18. Association of ACE Gene I/D polymorphism with migraine in Kashmiri population

    PubMed Central

    Wani, Irfan Yousuf; Sheikh, Saleem; Shah, Zafar Amin; Pandith, Arshid A.; Wani, Mushtaq; Asimi, Ravouf; Wani, Maqbool; Sheikh, Shahnawaz; Mehraj, Iqra

    2016-01-01

    Introduction: Migraine is a complex, recurrent headache disorder that is one of the most common complaints in neurology practice. The role of various genes in its pathogenesis is being studied. We did this study to see whether an association exists between ACE gene I/D polymorphism and migraine in our region. Materials and Methods: The study included 100 patients diagnosed with migraine and 121 healthy controls. The study subject were age and gender matched. The analysis was based on Polymerase Chain Reaction (PCR) and included following steps: DNA extraction from blood, PCR and Restriction Fragment Length Polymorphism (RFLP). Results: Out of 100 cases, 69 were females and 31 were males. Fifty-seven were having migraine without aura and 43 had migraine with aura. 45 of the cases had II polymorphism, 40 had ID polymorphism and 15 had DD polymorphism in ACE gene. Conclusion: We were not able to find a statistically significant association between ACE gene I/D polymorphism with migraine. The reason for difference in results between our study and other studies could be because of different ethnicity in study populations. So a continuous research is needed in this regard in order to find the genes and different polymorphism that increase the susceptibility of Kashmiri population to migraine. PMID:27011636

  19. Design and Technical Aspects of a New in Vacuum Transfocator at ESRF Beamline ID11

    SciTech Connect

    Rossat, M.; Vaughan, G. B. M.; Snigirev, A.; Wright, J. P; Bytchkov, A.; Dabin, Y.; Zhang, L.

    2010-06-23

    A tunable X-Ray focusing apparatus based on compound refractive lenses (CRL), referred to as a transfocator, has been installed for monochromatic beam (IAT in air transfocator) two years ago in the second optics hutch at ID11 at the ESRF. By varying the number of lenses in the beam, the energy focused and the focal length can be varied continuously throughout a large range of energies and distances.Based the success of the IAT, an in-vacuum transfocator (IVT) has now been built and installed at ID11.The aim of this poster is to describe and show the mechanical and technical solutions used to design and manufacture this new IVT.The IVT was installed last January in the first optics hutch at ID11. The IVT consists of nine water cooled cartridges containing 1, 2, 4, 8, 16, 32 Be lenses, and 32 and 64 Al lenses, and a protective aperture of Oe 900 {mu}m. The mechanical design is based on a system of pneumatically actuated cartridges with an auto positioning on two stainless steel rods. The vacuum vessel has translations and rotations perpendicular to the beam direction in order to align the axis of the cartridges with the x-ray beam.

  20. Id4, a New Candidate Gene for Senile Osteoporosis, Acts as a Molecular Switch Promoting Osteoblast Differentiation

    PubMed Central

    Yamashita, Yzumi; Nakachi, Yutaka; Nikaido, Itoshi; Bono, Hidemasa; Ninomiya, Yuichi; Kanesaki-Yatsuka, Yukiko; Akita, Masumi; Motegi, Hiromi; Wakana, Shigeharu; Noda, Tetsuo; Sablitzky, Fred; Arai, Shigeki; Kurokawa, Riki; Fukuda, Toru; Katagiri, Takenobu; Schönbach, Christian; Suda, Tatsuo; Mizuno, Yosuke; Okazaki, Yasushi

    2010-01-01

    Excessive accumulation of bone marrow adipocytes observed in senile osteoporosis or age-related osteopenia is caused by the unbalanced differentiation of MSCs into bone marrow adipocytes or osteoblasts. Several transcription factors are known to regulate the balance between adipocyte and osteoblast differentiation. However, the molecular mechanisms that regulate the balance between adipocyte and osteoblast differentiation in the bone marrow have yet to be elucidated. To identify candidate genes associated with senile osteoporosis, we performed genome-wide expression analyses of differentiating osteoblasts and adipocytes. Among transcription factors that were enriched in the early phase of differentiation, Id4 was identified as a key molecule affecting the differentiation of both cell types. Experiments using bone marrow-derived stromal cell line ST2 and Id4-deficient mice showed that lack of Id4 drastically reduces osteoblast differentiation and drives differentiation toward adipocytes. On the other hand knockdown of Id4 in adipogenic-induced ST2 cells increased the expression of Pparγ2, a master regulator of adipocyte differentiation. Similar results were observed in bone marrow cells of femur and tibia of Id4-deficient mice. However the effect of Id4 on Pparγ2 and adipocyte differentiation is unlikely to be of direct nature. The mechanism of Id4 promoting osteoblast differentiation is associated with the Id4-mediated release of Hes1 from Hes1-Hey2 complexes. Hes1 increases the stability and transcriptional activity of Runx2, a key molecule of osteoblast differentiation, which results in an enhanced osteoblast-specific gene expression. The new role of Id4 in promoting osteoblast differentiation renders it a target for preventing the onset of senile osteoporosis. PMID:20628571

  1. Understanding Pregnancy and Birth Issues

    MedlinePlus

    ... Navigation Bar Home Current Issue Past Issues Understanding Pregnancy and Birth Issues Past Issues / Winter 2008 Table ... turn Javascript on. What is a High-Risk Pregnancy? All pregnancies involve a certain degree of risk ...

  2. The Transcriptional Repressor ID2 Can Interact with the Canonical Clock Components CLOCK and BMAL1 and Mediate Inhibitory Effects on mPer1 Expression*

    PubMed Central

    Ward, Sarah M.; Fernando, Shanik J.; Hou, Tim Y.; Duffield, Giles E.

    2010-01-01

    ID2 is a rhythmically expressed HLH transcriptional repressor. Deletion of Id2 in mice results in circadian phenotypes, highlighted by disrupted locomotor activity rhythms and an enhanced photoentrainment response. ID2 can suppress the transactivation potential of the positive elements of the clock, CLOCK-BMAL1, on mPer1 and clock-controlled gene (CCG) activity. Misregulation of CCGs is observed in Id2−/− liver, and mutant mice exhibit associated alterations in lipid homeostasis. These data suggest that ID2 contributes to both input and output components of the clock and that this may be via interaction with the bHLH clock proteins CLOCK and BMAL1. The aim of the present study was to explore this potential interaction. Coimmunoprecipitation analysis revealed the capability of ID2 to complex with both CLOCK and BMAL1, and mammalian two-hybrid analysis revealed direct interactions of ID2, ID1 and ID3 with CLOCK and BMAL1. Deletion of the ID2 HLH domain rendered ID2 ineffective at inhibiting CLOCK-BMAL1 transactivation, suggesting that interaction between the proteins is via the HLH region. Immunofluorescence analysis revealed overlapping localization of ID2 with CLOCK and BMAL1 in the cytoplasm. Overexpression of CLOCK and BMAL1 in the presence of ID2 resulted in a significant reduction in their nuclear localization, revealing that ID2 can sequester CLOCK and BMAL1 to the cytoplasm. Serum stimulation of Id2−/− mouse embryonic fibroblasts resulted in an enhanced induction of mPer1 expression. These data provide the basis for a molecular mechanism through which ID2 could regulate aspects of both clock input and output through a time-of-day specific interaction with CLOCK and BMAL1. PMID:20861012

  3. You can be a wizard of IDS. Is your practice ready to form an integrated delivery system?

    PubMed

    Fabrizio, Nick A

    2007-09-01

    To combat declining reimbursement and strengthen their positions in a highly competitive provider environment, leaders of large medical groups may consider forming an integrate delivery system (IDS) to gain a business advantage. Managing the array of organizational, operational, financial and human relationships affected by an IDS requires careful planning, good timing and a bit of luck. This article, the first of a two-part series, examines what questions physician practice leaders should ask--and answer--before formiing an IDS. PMID:17910216

  4. High stability radio links

    NASA Technical Reports Server (NTRS)

    Kursinski, E. Robert

    1989-01-01

    Radio telecommunication links are used for communication with deep space probes. These links consist of sinusoidal carrier signals at radio frequencies (RF) modulated with information sent between the spacecraft and the earth. This carrier signal is a very pure and stable sinusoid, typically derived from an atomic frequency standard whose frequency and phase are used to measure the radial velocity of the probe and from this and other data types derive its trajectory. This same observable can be used to search for space-time distortions cased by low frequency (0.1 to 100 MHz) gravitation radiation. How such a system works, what its sensitivity limitations are, and what potential future improvements can be made are discussed.

  5. Linking Art and Books.

    ERIC Educational Resources Information Center

    McCoubrey, Sharon, Ed.

    1993-01-01

    This serial issue examines the theme of picture books as resources for art production and art response; gives specific project descriptions; and looks at the relationship between words and pictures, and the creative connection between art and language. Articles are: (1) "Editor's View" (Sharon McCoubrey); (2) "The Creative Connection: Art and…

  6. Peace--Iowa's Link.

    ERIC Educational Resources Information Center

    Gore, Deborah, Ed.

    1991-01-01

    This theme journal issue is devoted to an exploration of peace and its history in Iowa. The features and activities include: Iowa's Peace Tradition; A Global View; World Map; A Mesquakie Tale; Swords into Plowshares; Make a Peace Crane; Student Protest; Goldfinch Patriotism Debate; Peace Meal; A Short Story; and Work It Out. (DB)

  7. Australian Curriculum Linked Lessons

    ERIC Educational Resources Information Center

    Hurrell, Derek

    2013-01-01

    In providing a continued focus on tasks and activities that help to illustrate key ideas embedded in the new Australian Curriculum, the focus in this issue is on Measurement in the Measurement and Geometry strand. The small unit of work on measurement presented in this article has activities that can be modified to meet the requirements of…

  8. Australian Curriculum Linked Lessons

    ERIC Educational Resources Information Center

    Hurrell, Derek

    2014-01-01

    In providing a continued focus on tasks and activities that help to illustrate key ideas embedded in the new Australian Curriculum, this issue will focus on Number in the Number and Algebra strand. In this article Derek Hurrell provides a few tried and proven activities to develop place value understanding. These activities are provided for…

  9. Linking Service Learning & Standards.

    ERIC Educational Resources Information Center

    Degelman, Charles, Ed.

    2000-01-01

    This theme issue explores the connection between service learning and standards-based education. In the lead article, "Service Learning and Standards: Are They Compatible?" (Brian Loney), an educator applies his extensive experience with Active Citizenship Today, a successful social studies service-learning program, to a representative set of…

  10. Linking reengineering to revitalization.

    PubMed

    Moravec, M

    1996-01-01

    A health care organization that embarks on reengineering without a strategy for revitalizing is courting disaster. What does it take to get reengineering right? It takes vision, unflinching commitment, new kinds of leadership and business strategies, and new behaviors to challenge the old manager-physician power dynamic. A low-cost health care provider must be able to promote trust, partnerships, and competencies that are specifically linked to accessible, cost-effective patient care.

  11. Space Station Software Issues

    NASA Technical Reports Server (NTRS)

    Voigt, S. (Editor); Beskenis, S. (Editor)

    1985-01-01

    Issues in the development of software for the Space Station are discussed. Software acquisition and management, software development environment, standards, information system support for software developers, and a future software advisory board are addressed.

  12. Ethical issues in immunisation.

    PubMed

    Isaacs, David; Kilham, Henry; Leask, Julie; Tobin, Bernadette

    2009-01-29

    Discussions about current and future immunisation programmes raise novel questions about familiar ethical issues. Two sets of ethical issues dominate these discussions. The first is the issue of compulsory immunisation: what should be done about parents who fail to immunise their children? The second is: given competing demands on health care budgets, how should principles of justice in access and distribution inform vaccination programmes? This paper considers these two issues in the light of traditional ethical principles. With respect to the first, we argue that compulsion is justified only in cases in which we know with practical certainty that parental failure to immunise puts their own child or other children at high risk of severe illness. We also argue that the state should compensate those who suffer vaccine-related injury. With respect to the second, we claim that allocating resources according to health care need requires establishing priorities between public health programmes such as immunisation and other treatment programmes.

  13. Issues in Electronic Publishing.

    ERIC Educational Resources Information Center

    Meadow, Charles T.

    1997-01-01

    Discusses issues related to electronic publishing. Topics include writing; reading; production, distribution, and commerce; copyright and ownership of intellectual property; archival storage; technical obsolescence; control of content; equality of access; and cultural changes. (Author/LRW)

  14. Medical Issues: Orthopedics

    MedlinePlus

    ... support & care > living with sma > medical issues > orthopedics Orthopedics In SMA, muscle weakness can cause several complications. ... difficulty sitting, standing, or performing normal daily activities. Orthopedic Considerations Doctors and therapists classify individuals with SMA ...

  15. GROUND WATER SAMPLING ISSUES

    EPA Science Inventory

    Obtaining representative ground water samples is important for site assessment and
    remedial performance monitoring objectives. Issues which must be considered prior to initiating a ground-water monitoring program include defining monitoring goals and objectives, sampling point...

  16. Frontal Brain Electrical Activity (EEG) and Heart Rate in Response to Affective Infant-Directed (ID) Speech in 9-Month-Old Infants

    ERIC Educational Resources Information Center

    Santesso, Diane L.; Schmidt, Louis A.; Trainor, Laurel J.

    2007-01-01

    Many studies have shown that infants prefer infant-directed (ID) speech to adult-directed (AD) speech. ID speech functions to aid language learning, obtain and/or maintain an infant's attention, and create emotional communication between the infant and caregiver. We examined psychophysiological responses to ID speech that varied in affective…

  17. DnsID in MyCompoundID for rapid identification of dansylated amine- and phenol-containing metabolites in LC-MS-based metabolomics.

    PubMed

    Huan, Tao; Wu, Yiman; Tang, Chenqu; Lin, Guohui; Li, Liang

    2015-10-01

    High-performance chemical isotope labeling (CIL) liquid chromatography-mass spectrometry (LC-MS) is an enabling technology based on rational design of labeling reagents to target a class of metabolites sharing the same functional group (e.g., all the amine-containing metabolites or the amine submetabolome) to provide concomitant improvements in metabolite separation, detection, and quantification. However, identification of labeled metabolites remains to be an analytical challenge. In this work, we describe a library of labeled standards and a search method for metabolite identification in CIL LC-MS. The current library consists of 273 unique metabolites, mainly amines and phenols that are individually labeled by dansylation (Dns). Some of them produced more than one Dns-derivative (isomers or multiple labeled products), resulting in a total of 315 dansyl compounds in the library. These metabolites cover 42 metabolic pathways, allowing the possibility of probing their changes in metabolomics studies. Each labeled metabolite contains three searchable parameters: molecular ion mass, MS/MS spectrum, and retention time (RT). To overcome RT variations caused by experimental conditions used, we have developed a calibration method to normalize RTs of labeled metabolites using a mixture of RT calibrants. A search program, DnsID, has been developed in www.MyCompoundID.org for automated identification of dansyl labeled metabolites in a sample based on matching one or more of the three parameters with those of the library standards. Using human urine as an example, we illustrate the workflow and analytical performance of this method for metabolite identification. This freely accessible resource is expandable by adding more amine and phenol standards in the future. In addition, the same strategy should be applicable for developing other labeled standards libraries to cover different classes of metabolites for comprehensive metabolomics using CIL LC-MS.

  18. Ecological issues related to ozone: agricultural issues.

    PubMed

    Fuhrer, Jürg; Booker, Fitzgerald

    2003-06-01

    Research on the effects of ozone on agricultural crops and agro-ecosystems is needed for the development of regional emission reduction strategies, to underpin practical recommendations aiming to increase the sustainability of agricultural land management in a changing environment, and to secure food supply in regions with rapidly growing populations. Major limitations in current knowledge exist in several areas: (1) Modelling of ozone transfer and specifically stomatal ozone uptake under variable environmental conditions, using robust and well-validated dynamic models that can be linked to large-scale photochemical models lack coverage. (2) Processes involved in the initial reactions of ozone with extracellular and cellular components after entry through the stomata, and identification of key chemical species and their role in detoxification require additional study. (3) Scaling the effects from the level of individual cells to the whole-plant requires, for instance, a better understanding of the effects of ozone on carbon transport within the plant. (4) Implications of long-term ozone effects on community and whole-ecosystem level processes, with an emphasis on crop quality, element cycling and carbon sequestration, and biodiversity of pastures and rangelands require renewed efforts. The UNECE Convention on Long Range Trans-boundary Air Pollution shows, for example, that policy decisions may require the use of integrated assessment models. These models depend on quantitative exposure-response information to link quantitative effects at each level of organization to an effective ozone dose (i.e., the balance between the rate of ozone uptake by the foliage and the rate of ozone detoxification). In order to be effective in a policy, or technological context, results from future research must be funnelled into an appropriate knowledge transfer scheme. This requires data synthesis, up-scaling, and spatial aggregation. At the research level, interactions must be

  19. DOIs Should Not Link to Data

    NASA Astrophysics Data System (ADS)

    Hourcle, J. A.

    2013-12-01

    In August of 2011, the National Academy of Science's Board of Research Data and Information (BRDI) had a meeting on the topic of "Developing Data Attribution and Citation Practices and Standards". As part of that meeting were various breakout groups to discuss issues that still remained regarding technical, scientific, and socio-cultural issues, the roles and actors that needed to be involved, and how to get additional feedback from the community. The technical breakout group decided that the majority of the technical problems with citing data revolved around establishing the identity of what it was that we were attempting to cite. As such, our recommendations were to push some of the work back onto the data publishers to tell us how scientists should cite their data (to avoid different disciplines applying different rules), establish 'data landing pages' to describe, document and link to the data, and to provide those pages with persistent identifiers such as a DOI. We present here some of the reasons that were discussed about why not to link directly to data, including: * recalibration, reaccessioning and other data impermanence, * setting context and providing for links to documentation, and * allowing selection of different packaging formats

  20. Determination of trace elements on polysilicates by ID-ICP-MS with ultrasonic nebulization/membrane desolvation

    SciTech Connect

    Bonchin-Cleland, S.; Olivares, J.A.; Miller, G.G.; Gallegos, L.; Dawson, H.J.

    1996-06-01

    This work investigates the performance of an ID-ICP-MS (isotope dilution-inductively coupled plasma mass spectrometry) system with USN/MD (ultrasonic nebulization/membrane desolvation) sample introduction for the determination of trace metal impurities in polysilicon.

  1. Earth observing system. Output data products and input requirements, version 2.0. Volume 2: Analysis of IDS input requirements

    NASA Technical Reports Server (NTRS)

    Lu, Yun-Chi; Chang, Hyo Duck; Krupp, Brian; Kumar, Ravindra; Swaroop, Anand

    1992-01-01

    On 18 Jan. 1991, NASA confirmed 29 Inter-Disciplinary Science (IDS) teams, each involving a group of investigators, to conduct interdisciplinary research using data products from Earth Observing System (EOS) instruments. These studies are multi-disciplinary and require output data products from multiple EOS instruments, including both FI and PI instruments. The purpose of this volume is to provide information on output products expected from IDS investigators, required input data, and retrieval algorithms. Also included in this volume is the revised analysis of the 'best' and 'alternative' match data products for IDS input requirements. The original analysis presented in the August 1991 release of the SPSO Report was revised to incorporate the restructuring of the EOS platform. As a result of the reduced EOS payload, some of EOS instruments were deselected and their data products would not be available for IDS research. Information on these data products is also presented.

  2. FAA Technical Center Aeronautical Data Link Research Plan

    NASA Astrophysics Data System (ADS)

    Buck, Frank; Cratch, Preston; Fischer, Terence; Lunder, Joseph; Shingledecker, Clark; Stahl, David; Sweeney, David

    1992-10-01

    The purpose of this plan is to project a clear and distinct description of the Data Link research that is to be conducted at the Federal Aviation Administration (FAA) Technical Center over the next 4 years. It explicitly defines what is to be achieved at a specific time in the future. End-to-end, high fidelity simulations will be the primary methodology for answering research questions. The end-to-end simulations identified in this plan are intended to investigate controller/aircrew integration issues using candidate Data Link hardware and software configurations. Research will also focus on testing Data Link applications in terms of their impact on controllers, aircrew, and the overall safety, efficiency, and productivity of the system. Additionally, research efforts are planned to address the most critical human factors issues surrounding Data Link.

  3. See Also:Mechanics of Cohesive-frictional MaterialsCopyright © 2004 John Wiley & Sons, Ltd.Get Sample Copy

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    NASA Astrophysics Data System (ADS)

    Bae, G. J.; Shin, H. S.; Sicilia, C.; Choi, Y. G.; Lim, J. J.

    2005-01-01

    This paper deals with the grouted pipe-roofing reinforcement method that is used in the construction of tunnels through weak grounds. This system consists on installing, prior to the excavation of a length of tunnel, an array of pipes forming a kind of umbrella above the area to be excavated. In some cases, these pipes are later used to inject grout to strengthen the ground and connect the pipes.This system has proven to be very efficient in reducing tunnel convergence and water inflow when tunnelling through weak grounds. However, due to the geometrical and mechanical complexity of the problem, existing finite element frameworks are inappropriate to simulate tunnelling using this method.In this paper, a mathematical framework based on a homogenization technique to simulate grouted pipe-roofing reinforced ground and its implementation into a 3-D finite element programme that can consider stage construction situations are presented. The constitutive model developed allows considering the main design parameters of the problem and only requires geometrical and mechanical properties of the constituents. Additionally, the use of a homogenization approach implies that the generation of the finite element mesh can be easily produced and that re-meshing is not required as basic geometrical parameters such as the orientation of the pipes are changed.The model developed is used to simulate tunnelling with the grouted pipe-roofing reinforcement method. From the analyses, the effects of the main design parameters on the elastic and the elastoplastic analyses are considered. Copyright

  6. See Also:Mechanics of Cohesive-frictional MaterialsCopyright © 2004 John Wiley & Sons, Ltd.Get Sample Copy

  7. Recommend to Your Librarian
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    NASA Astrophysics Data System (ADS)

    Jourine, S.; Valkó, P. P.; Kronenberg, A. K.

    2004-10-01

    A general poroelastic solution for axisymmetrical plane strain problems with time dependent boundary conditions is developed in Laplace domain. Time-domain results are obtained using numerical inversion of the Laplace transform. Previously published solutions can be considered as special cases of the proposed solution. In particular, we could reproduce numerical results for solid and hollow poroelastic cylinders with suddenly applied load/pressure (Rice and Cleary, Rev. Geophys. Space Phys. 1976; 14:227; Schmitt, Tait and Spann, Int. J. Rock Mech. Min. Sci. 1993; 30:1057; Cui and Abousleiman, ASCE J. Eng. Mech. 2001; 127:391).The new solution is used to model laboratory tests on thick-walled hollow cylinders of Berea sandstone subjected to intensive pressure drawdown. In the experiments, pressure at the inner boundary of the hollow cylinder is observed to decline exponentially with a decay constant of 3-5 1/s.It is found that solutions with idealized step-function type inner boundary conditions overestimate the induced tensile radial stresses considerably. Although basic poroelastic phenomena can be modelled properly at long time following a stepwise change in pressure, realistic time varying boundary conditions predict actual rock behaviour better at early time. Experimentally observed axial stresses can be matched but appear to require different values for α and than are measured at long time.The proposed solution can be used to calculate the stress and pore pressure distributions around boreholes under infinite/finite boundary conditions. Prospective applications include investigating the effect of gradually changing pore pressure, modelling open-hole cavity completions, and describing the phenomenon of wellbore collapse (bridging) during oil or gas blowouts. Copyright

  9. Bypassing STAT3-mediated inhibition of the transcriptional regulator ID2 improves the antitumor efficacy of dendritic cells.

    PubMed

    Li, Haiyan S; Liu, Chengwen; Xiao, Yichuan; Chu, Fuliang; Liang, Xiaoxuan; Peng, Weiyi; Hu, Jianhua; Neelapu, Sattva S; Sun, Shao-Cong; Hwu, Patrick; Watowich, Stephanie S

    2016-01-01

    Despite the potent ability of dendritic cells (DCs) to stimulate lymphocyte responses and host immunity, granulocyte-macrophage colony-stimulating factor-derived DCs (GM-DCs) used as antitumor vaccines have demonstrated relatively modest success in cancer immunotherapy. We found that injecting GM-DCs into melanoma tumors in mice, or culturing GM-DCs with melanoma-secreted cytokines or melanoma-conditioned medium, rapidly suppressed DC-intrinsic expression of the gene encoding inhibitor of differentiation 2 (ID2), a transcriptional regulator. Melanoma-associated cytokines repressed Id2 transcription in murine DCs through the activation of signal transducer and activator of transcription 3 (STAT3). Enforced expression of ID2 in GM-DCs (ID2-GM-DCs) suppressed their production of the proinflammatory cytokine tumor necrosis factor-α (TNF-α). Vaccination with ID2-GM-DCs slowed the progression of melanoma tumors and enhanced animal survival, which was associated with an increased abundance of tumor-infiltrating interferon-γ-positive CD4(+) effector and CD8(+) cytotoxic T cells and a decreased number of tumor-infiltrating regulatory CD4(+) T cells. The efficacy of the ID2-GM-DC vaccine was improved by combinatorial treatment with a blocking antibody to programmed cell death protein-1 (PD-1), a current immunotherapy that overcomes suppressive immune checkpoint signaling. Collectively, our data reveal a previously unrecognized STAT3-mediated immunosuppressive mechanism in DCs and indicate that DC-intrinsic ID2 promotes tumor immunity by modulating tumor-associated CD4(+) T cell responses. Thus, inhibiting STAT3 or overexpressing ID2 selectively in DCs may improve the efficiency of DC vaccines in cancer therapy. PMID:27678219

  10. Linking ultracold polar molecules.

    PubMed

    Avdeenkov, A V; Bohn, John L

    2003-01-31

    We predict that pairs of polar molecules can be weakly bound together in an ultracold environment, provided that a dc electric field is present. The field that links the molecules together also strongly influences the basic properties of the resulting dimer, such as its binding energy and predissociation lifetime. Because of their long-range character, these dimers will be useful in disentangling cold collision dynamics of polar molecules. As an example, we estimate the microwave photoassociation yield for OH-OH cold collisions.

  11. EEE Links. Volume 5

    NASA Technical Reports Server (NTRS)

    Humphrey, Robert (Editor)

    1999-01-01

    The EEE Links Newsletter is a quarterly publication produced by Code 562 in support of the NASA HQ funded NASA Electronic Parts and Packaging (NEPP) Program. The newsletter is produced as an electronic format deliverable made available via the referenced www site administered by Code 562, The newsletter publishes brief articles on topics of interest to NASA programs and projects in the area of electronic parts and packaging. The newsletter does not provide information pertaining to patented or proprietary information. The information provided is at the level of that produced by industry and university researchers and is published at national and international conferences.

  12. Nuclear data production, calculation and measurement: a global overview of the gamma heating issue

    NASA Astrophysics Data System (ADS)

    Colombier, A.-C.; Amharrak, H.; Fourmentel, D.; Ravaux, S.; Régnier, D.; Gueton, O.; Hudelot, J.-P.; Lemaire, M.

    2013-03-01

    The gamma heating evaluation in different materials found in current and future generations of nuclear reactor (EPRTM, GENIV, MTR-JHR), is becoming an important issue especially for the design of many devices (control rod, heavy reflector, in-core & out-core experiments…). This paper deals with the works started since 2009 in the Reactor Studies Department of CEA Cadarache in ordre to answer to several problematic which have been identified as well for nuclear data production and calculation as for experimental measurement methods. The selected subjects are: id="bid.1"> Development of a Monte Carlo code (FIFRELIN) to simulate the prompt fission gamma emission which represents the major part of the gamma heating production inside the core id="bid.2"> Production and qualification of new evaluations of nuclear data especially for radiative capture and inelastic neutron scattering which are the main sources of gamma heating out-core id="bid.3"> Development and qualification of a recommended method for the total gamma heating calculation using the Monte Carlo simulation code TRIPOLI-4 id="bid.4"> Development, test and qualification of new devices dedicated to the in-core gamma heating measurement as well in MTR-JHR as in zero power facilities (EOLE-MINERVE) of CEA, Cadarache to increase the experimental measurement accuracy.

  13. Wide allelic heterogeneity with predominance of large IDS gene complex rearrangements in a sample of Mexican patients with Hunter syndrome.

    PubMed

    Alcántara-Ortigoza, M A; García-de Teresa, B; González-Del Angel, A; Berumen, J; Guardado-Estrada, M; Fernández-Hernández, L; Navarrete-Martínez, J I; Maza-Morales, M; Rius-Domínguez, R

    2016-05-01

    Hunter syndrome or mucopolysaccharidosis type II (MPSII) is caused by pathogenic variants in the IDS gene. This is the first study that examines the mutational spectrum in 25 unrelated Mexican MPSII families. The responsible genotype was identified in 96% of the families (24/25) with 10 novel pathogenic variants: c.133G>C, c.1003C>T, c.1025A>C, c.463_464delinsCCGTATAGCTGG, c.754_767del, c.1132_1133del, c.1463del, c.508-1G>C, c.1006+1G>T and c.(-217_103del). Extensive IDS gene deletions were identified in four patients; using DNA microarray analysis two patients showed the loss of the entire AFF2 gene, and epilepsy developed in only one of them. Wide allelic heterogeneity was noted, with large gene alterations (e.g. IDS/IDSP1 gene inversions, partial to extensive IDS deletions, and one chimeric IDS-IDSP1 allele) that occurred at higher frequencies than previously reported (36% vs 18.9-29%). The frequency of carrier mothers (80%) is consistent with previous descriptions (>70%). Carrier assignment allowed molecular prenatal diagnoses. Notably, somatic and germline mosaicism was identified in one family, and two patients presented thrombocytopenic purpura and pancytopenia after idursulfase enzyme replacement treatment. Our findings suggest a wide allelic heterogeneity in Mexican MPSII patients; DNA microarray analysis contributes to further delineation of the resulting phenotype for IDS and neighboring loci deletions.

  14. Wide allelic heterogeneity with predominance of large IDS gene complex rearrangements in a sample of Mexican patients with Hunter syndrome.

    PubMed

    Alcántara-Ortigoza, M A; García-de Teresa, B; González-Del Angel, A; Berumen, J; Guardado-Estrada, M; Fernández-Hernández, L; Navarrete-Martínez, J I; Maza-Morales, M; Rius-Domínguez, R

    2016-05-01

    Hunter syndrome or mucopolysaccharidosis type II (MPSII) is caused by pathogenic variants in the IDS gene. This is the first study that examines the mutational spectrum in 25 unrelated Mexican MPSII families. The responsible genotype was identified in 96% of the families (24/25) with 10 novel pathogenic variants: c.133G>C, c.1003C>T, c.1025A>C, c.463_464delinsCCGTATAGCTGG, c.754_767del, c.1132_1133del, c.1463del, c.508-1G>C, c.1006+1G>T and c.(-217_103del). Extensive IDS gene deletions were identified in four patients; using DNA microarray analysis two patients showed the loss of the entire AFF2 gene, and epilepsy developed in only one of them. Wide allelic heterogeneity was noted, with large gene alterations (e.g. IDS/IDSP1 gene inversions, partial to extensive IDS deletions, and one chimeric IDS-IDSP1 allele) that occurred at higher frequencies than previously reported (36% vs 18.9-29%). The frequency of carrier mothers (80%) is consistent with previous descriptions (>70%). Carrier assignment allowed molecular prenatal diagnoses. Notably, somatic and germline mosaicism was identified in one family, and two patients presented thrombocytopenic purpura and pancytopenia after idursulfase enzyme replacement treatment. Our findings suggest a wide allelic heterogeneity in Mexican MPSII patients; DNA microarray analysis contributes to further delineation of the resulting phenotype for IDS and neighboring loci deletions. PMID:26762690

  15. Ethical issues in obstetrics.

    PubMed

    Digiovanni, Laura M

    2010-06-01

    Obstetricians must become comfortable addressing the ethical issues involved in clinical obstetrics and therefore must have an understanding of the key elements of clinical medical ethics. Balancing the principles of medical ethics can guide clinicians toward solutions to ethical dilemmas encountered in the care of pregnant women. The purpose of this article is to review the ethical foundations of clinical practice, recognize the ethical issues obstetricians face every day in caring for patients, and facilitate decision making. This article discusses the relevant ethical principles, identifies unique features of obstetrical ethics, examines ethical principles as they apply to pregnant patient and her fetus, and thereby, provides a conceptual framework for considering ethical issues and facilitating decision making in clinical obstetrics.

  16. Special Issue: "Functional Dendrimers".

    PubMed

    Tomalia, Donald A

    2016-01-01

    This special issue entitled "Functional Dendrimers" focuses on the manipulation of at least six "critical nanoscale design parameters" (CNDPs) of dendrimers including: size, shape, surface chemistry, flexibility/rigidity, architecture and elemental composition. These CNDPs collectively define properties of all "functional dendrimers". This special issue contains many interesting examples describing the manipulation of certain dendrimer CNDPs to create new emerging properties and, in some cases, predictive nanoperiodic property patterns (i.e., dendritic effects). The systematic engineering of CNDPs provides a valuable strategy for optimizing functional dendrimer properties for use in specific applications. PMID:27517890

  17. SpaceWire Protocol ID: What Does It Mean To You?

    NASA Technical Reports Server (NTRS)

    Rakow, Glenn; Schnurr, Richard; Gilley, Daniel; Parks, Steve

    2006-01-01

    packet format adds one new field to the packet that greatly enhances the capability of Spacewire. This new field called the Protocol Identifier (ID) is used to identify the packet contents and the associated processing for the packet. This feature along with the restriction in the packet format that uses the Protocol ID, allows a deterministic method of decoding packets that was not before possible. The first part of the packet is still the Destination Address, which still conforms to the original standard but with one restriction. The restriction is that the first byte seen at the destination by the user needs to be a logical address, independent of the addressing scheme used. The second field is defined as the Protocol ID, which is usually one byte in length. The packet cargo (user defined) follows the Protocol ID. After the packet cargo is the EOP, which defines the end of packet. The value of the Protocol ID is assigned by the Spacewire working group and the protocol description published for others to use. The development of Protocols for Spacewire is currently the area of greatest activity by the Spacewire working group. The first protocol definition by the working group has been completed and is now in the process of formal standardization. There are many other protocols in development for missions that have not yet received formal Protocol ID assignment, but even if the protocols are not formally assigned a value, this effort will provide synergism for future developments.

  18. Does extending the dual-task functional exercises workout improve postural balance in individuals with ID?

    PubMed

    Mikolajczyk, Edyta; Jankowicz-Szymanska, Agnieszka

    2015-03-01

    Maintaining postural balance, overcoming visual and motor coordination disorders and experiencing problems with low general fitness - typical of intellectually disabled individuals - adversely affect the performance quality of their activities of daily living (ADLs). Physical fitness and postural balance can be improved by taking part in special intervention programs. Our study was designed to test whether extending the dual-task intervention program (combining ADLs with balance exercises on unstable surfaces) from 12 to 24 weeks additionally improved postural balance in individuals with intellectual disability (ID). We also attempted to assess whether the effects of the above intervention program were still noticeable after 8 weeks of holidays, in which participants did not take any rehabilitation exercises. A total of 34 adolescents, aged 14-16 years (15.06±0.9), with moderate ID took part in our study. The experimental group (E) consisted of 17 individuals, who continued the intervention program originated 3 months earlier, and the control group (C) comprised the same number of participants. Postural balance was assessed on a stabilometric platform Alfa. Having extended the workout period by another 12 weeks, we noticed that the path length of the center of pressure (COP) covered by participants on tests with their eyes open and closed significantly shortened. After a lapse of 8 weeks from the completion of the program, the experimental group revealed a statistically significant decrease in the velocity along the medio-lateral (M/L) and anterior-posterior (A/P) axes. The remaining variables stayed at the same level and the control group did not demonstrate any statistically significant changes. Dual-task exercises, in which enhancing functional tasks of daily living is combined with a parallel stimulation of balance reactions, may improve static balance in persons with ID.

  19. ID-SERS Based Reference Method for Quantification of Large Biomolecules on a Single Chip

    SciTech Connect

    Yaghobian, Fatemeh; Stosch, Rainer; Henrion, Andre; Guettler, Bernd

    2010-08-06

    Accuracy and precision of quantitative SERS results have been significantly increased by applying a method based on the so-called isotope-dilution (ID) principle. In this ID-SERS approach, an isotopically labeled analogue of the target molecule (isotopologue) is spiked to the sample at a known concentration. Due to the slight difference in their molar masses, some Raman bands of the heavier isotopologue are red-shifted with respect to the same signals resulting from the unlabelled compound. As a result, spectra evaluation is reduced to the determination of intensity ratios rather than absolute intensities, and the unknown quantity of the analyte can be calculated from the known quantity of the standard. This procedure is of particular interest in the development of highly accurate reference procedures for metrology in chemistry. Because the sample is spiked prior to any further treatment, potential loss of material or matrix effects would equally affect both isotopologues, without influencing the final result. The method has been successfully applied for quantifying small diagnostic marker molecules like creatinine at their relevant serum concentration levels using silver colloids as SERS substrates. Now, the ID-SERS approach has been realized as a 'one-chip' approach using 'Bio-chips' made of intrinsically grown spherical silver nanoparticles with gaps less than 10 nm in between (Fig. 1). In addition, the scope of the method has been extended to larger biomolecules like peptides which will be shown using the example of the human growth-hormone (hGH) peptide T12 at physiologically relevant serum concentration levels (Fig. 2). Further developments towards the quantification of full proteins will also be reported.

  20. ID-SERS Based Reference Method for Quantification of Large Biomolecules on a Single Chip

    NASA Astrophysics Data System (ADS)

    Yaghobian, Fatemeh; Stosch, Rainer; Henrion, André; Güttler, Bernd

    2010-08-01

    Accuracy and precision of quantitative SERS results have been significantly increased by applying a method based on the so-called isotope-dilution (ID) principle. In this ID-SERS approach, an isotopically labeled analogue of the target molecule (isotopologue) is spiked to the sample at a known concentration. Due to the slight difference in their molar masses, some Raman bands of the heavier isotopologue are red-shifted with respect to the same signals resulting from the unlabelled compound. As a result, spectra evaluation is reduced to the determination of intensity ratios rather than absolute intensities, and the unknown quantity of the analyte can be calculated from the known quantity of the standard. This procedure is of particular interest in the development of highly accurate reference procedures for metrology in chemistry. Because the sample is spiked prior to any further treatment, potential loss of material or matrix effects would equally affect both isotopologues, without influencing the final result. The method has been successfully applied for quantifying small diagnostic marker molecules like creatinine at their relevant serum concentration levels using silver colloids as SERS substrates. Now, the ID-SERS approach has been realized as a "one-chip" approach using "Bio-chips" made of intrinsically grown spherical silver nanoparticles with gaps less than 10 nm in between (Fig. 1). In addition, the scope of the method has been extended to larger biomolecules like peptides which will be shown using the example of the human growth-hormone (hGH) peptide T12 at physiologically relevant serum concentration levels (Fig. 2). Further developments towards the quantification of full proteins will also be reported.

  21. kpath: integration of metabolic pathway linked data.

    PubMed

    Navas-Delgado, Ismael; García-Godoy, María Jesús; López-Camacho, Esteban; Rybinski, Maciej; Reyes-Palomares, Armando; Medina, Miguel Ángel; Aldana-Montes, José F

    2015-01-01

    In the last few years, the Life Sciences domain has experienced a rapid growth in the amount of available biological databases. The heterogeneity of these databases makes data integration a challenging issue. Some integration challenges are locating resources, relationships, data formats, synonyms or ambiguity. The Linked Data approach partially solves the heterogeneity problems by introducing a uniform data representation model. Linked Data refers to a set of best practices for publishing and connecting structured data on the Web. This article introduces kpath, a database that integrates information related to metabolic pathways. kpath also provides a navigational interface that enables not only the browsing, but also the deep use of the integrated data to build metabolic networks based on existing disperse knowledge. This user interface has been used to showcase relationships that can be inferred from the information available in several public databases. PMID:26055101

  22. kpath: integration of metabolic pathway linked data.

    PubMed

    Navas-Delgado, Ismael; García-Godoy, María Jesús; López-Camacho, Esteban; Rybinski, Maciej; Reyes-Palomares, Armando; Medina, Miguel Ángel; Aldana-Montes, José F

    2015-01-01

    In the last few years, the Life Sciences domain has experienced a rapid growth in the amount of available biological databases. The heterogeneity of these databases makes data integration a challenging issue. Some integration challenges are locating resources, relationships, data formats, synonyms or ambiguity. The Linked Data approach partially solves the heterogeneity problems by introducing a uniform data representation model. Linked Data refers to a set of best practices for publishing and connecting structured data on the Web. This article introduces kpath, a database that integrates information related to metabolic pathways. kpath also provides a navigational interface that enables not only the browsing, but also the deep use of the integrated data to build metabolic networks based on existing disperse knowledge. This user interface has been used to showcase relationships that can be inferred from the information available in several public databases.

  23. Drugs + HIV, Learn the Link

    MedlinePlus

    ... Children & Teens Search Connect with NIDA : Google Plus Facebook LinkedIn Twitter YouTube Flickr RSS Menu Home Drugs ... HIV Learn the Link - Drugs and HIV Email Facebook Twitter 2005 –Ongoing Behaviors associated with drug abuse ...

  24. PREFACE: CEWQO Topical Issue CEWQO Topical Issue

    NASA Astrophysics Data System (ADS)

    Bozic, Mirjana; Man'ko, Margarita

    2009-09-01

    This topical issue of Physica Scripta collects selected peer-reviewed contributions based on invited and contributed talks and posters presented at the 15th Central European Workshop on Quantum Optics (CEWQO) which took place in Belgrade 29 May-3 June 2008 (http://cewqo08.phy.bg.ac.yu). On behalf of the whole community took place in Belgrade 29 May-3 June 2008 (http://cewqo08.phy.bg.ac.yu, cewqo08.phy.bg.ac.yu). On behalf of the whole community of the workshop, we thank the referees for their careful reading and useful suggestions which helped to improve all of the submitted papers. A brief description of CEWQO The Central European Workshop on Quantum Optics is a series of conferences started informally in Budapest in 1992. Sometimes small events transform into important conferences, as in the case of CEWQO. Professor Jozsef Janszky, from the Research Institute of Solid State Physics and Optics, is the founder of this series. Margarita Man'ko obtained the following information from Jozsef Janszky during her visit to Budapest, within the framework of cooperation between the Russian and Hungarian Academies of Sciences in 2005. He organized a small workshop on quantum optics in Budapest in 1992 with John Klauder as a main speaker. Then, bearing in mind that a year before Janszky himself was invited by Vladimir Buzek to give a seminar on the same topic in Bratislava, he decided to assign the name 'Central European Workshop on Quantum Optics', considering the seminar in Bratislava to be the first workshop and the one in Budapest the second. The third formal workshop took place in Bratislava in 1993 organized by Vladimir Buzek, then in 1994 (Budapest, by Jozsef Janszky), 1995 and 1996 (Budmerice, Slovakia, by Vladimir Buzek), 1997 (Prague, by Igor Jex), 1999 (Olomouc, Czech Republic, by Zdenek Hradil), 2000 (Balatonfüred, Hungary, by Jozsef Janszky ), 2001 (Prague, by Igor Jex), 2002 (Szeged, Hungary, by Mihaly Benedict), 2003 (Rostock,Germany, by Werner Vogel and