Sample records for identification procedure based

  1. Mould routine identification in the clinical laboratory by matrix-assisted laser desorption ionization time-of-flight mass spectrometry.

    PubMed

    Cassagne, Carole; Ranque, Stéphane; Normand, Anne-Cécile; Fourquet, Patrick; Thiebault, Sandrine; Planard, Chantal; Hendrickx, Marijke; Piarroux, Renaud

    2011-01-01

    MALDI-TOF MS recently emerged as a valuable identification tool for bacteria and yeasts and revolutionized the daily clinical laboratory routine. But it has not been established for routine mould identification. This study aimed to validate a standardized procedure for MALDI-TOF MS-based mould identification in clinical laboratory. First, pre-extraction and extraction procedures were optimized. With this standardized procedure, a 143 mould strains reference spectra library was built. Then, the mould isolates cultured from sequential clinical samples were prospectively subjected to this MALDI-TOF MS based-identification assay. MALDI-TOF MS-based identification was considered correct if it was concordant with the phenotypic identification; otherwise, the gold standard was DNA sequence comparison-based identification. The optimized procedure comprised a culture on sabouraud-gentamicin-chloramphenicol agar followed by a chemical extraction of the fungal colonies with formic acid and acetonitril. The identification was done using a reference database built with references from at least four culture replicates. For five months, 197 clinical isolates were analyzed; 20 were excluded because they were not identified at the species level. MALDI-TOF MS-based approach correctly identified 87% (154/177) of the isolates analyzed in a routine clinical laboratory activity. It failed in 12% (21/177), whose species were not represented in the reference library. MALDI-TOF MS-based identification was correct in 154 out of the remaining 156 isolates. One Beauveria bassiana was not identified and one Rhizopus oryzae was misidentified as Mucor circinelloides. This work's seminal finding is that a standardized procedure can also be used for MALDI-TOF MS-based identification of a wide array of clinically relevant mould species. It thus makes it possible to identify moulds in the routine clinical laboratory setting and opens new avenues for the development of an integrated MALDI-TOF MS-based solution for the identification of any clinically relevant microorganism.

  2. Identification of Printed Nonsense Words for an Individual with Autism: A Comparison of Constant Time Delay and Stimulus Fading

    ERIC Educational Resources Information Center

    Redhair, Emily

    2011-01-01

    This study compared a stimulus fading (SF) procedure with a constant time delay (CTD) procedure for identification of consonant-vowel-consonant (CVC) nonsense words for a participant with autism. An alternating treatments design was utilized through a computer-based format. Receptive identification of target words was evaluated using a computer…

  3. Identification of Printed Nonsense Words for an Individual with Autism: A Comparison of Constant Time Delay and Stimulus Fading

    ERIC Educational Resources Information Center

    Redhair, Emily I.; McCoy, Kathleen M.; Zucker, Stanley H.; Mathur, Sarup R.; Caterino, Linda

    2013-01-01

    This study compared a stimulus fading (SF) procedure with a constant time delay (CTD) procedure for identification of consonant-vowel-consonant (CVC) nonsense words for a participant with autism. An alternating treatments design was utilized through a computer-based format. Receptive identification of target words was evaluated using a computer…

  4. Direct structural parameter identification by modal test results

    NASA Technical Reports Server (NTRS)

    Chen, J.-C.; Kuo, C.-P.; Garba, J. A.

    1983-01-01

    A direct identification procedure is proposed to obtain the mass and stiffness matrices based on the test measured eigenvalues and eigenvectors. The method is based on the theory of matrix perturbation in which the correct mass and stiffness matrices are expanded in terms of analytical values plus a modification matrix. The simplicity of the procedure enables real time operation during the structural testing.

  5. A survey on the utility of the USEPA CADDIS stressor identification procedure.

    PubMed

    Harwood, John J; Stroud, Robert Adam

    2012-06-01

    The Environmental Protection Agency (EPA) has made available on the worldwide web a systematic stream stressor identification procedure, the "Causal Analysis/Diagnosis Decision Information System" or CADDIS. We report here the results of a survey of regulators and scientists in 11 states who use CADDIS or another stressor identification procedure in their work. The 13 survey questions address guidelines as to what impairment scenarios to approach with stressor identification, what information is needed to perform stressor identification, and what the stakeholder role is in performing stressor identification. At the time of this survey (the summer of 2009), the EPA CADDIS website was less commonly used among the state regulators surveyed than the published EPA stressor identification document on which it is based. The respondents generally find the EPA stressor identification procedure useful and capable of being adapted to their individual needs. Survey respondents all use stressor identification in their Total Maximum Daily Load work, but also in a wide variety of other applications. All the "types of evidence" included in the CADDIS stressor identification procedure are used by the practitioners surveyed with the exception of the results of ecological simulation models. While the CADDIS documentation encourages the involvement of stakeholders in stressor identification, most respondents do not assemble stakeholder teams of local officials and citizens to participate in stressor analyses.

  6. Community-Based Efforts to Increase the Identification of the Number of Gifted Minority Children.

    ERIC Educational Resources Information Center

    Davis, Paul I.

    The document reports on a study of community-based identification of gifted and talented Black middle-school students, as an adjunct to formal identification procedures. A developmental framework for the identification of the gifted minority child was distributed to 17 known leaders in the Black community (including ministers, youth leaders in…

  7. 31 CFR 1024.220 - Customer identification programs for mutual funds.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... Identification Program (“CIP”) appropriate for its size and type of business that, at a minimum, includes each of the requirements of paragraphs (a)(1) through (5) of this section. The CIP must be a part of the...). (2) Identity verification procedures. The CIP must include risk-based procedures for verifying the...

  8. 31 CFR 1024.220 - Customer identification programs for mutual funds.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... Identification Program (“CIP”) appropriate for its size and type of business that, at a minimum, includes each of the requirements of paragraphs (a)(1) through (5) of this section. The CIP must be a part of the...). (2) Identity verification procedures. The CIP must include risk-based procedures for verifying the...

  9. 31 CFR 1024.220 - Customer identification programs for mutual funds.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... Identification Program (“CIP”) appropriate for its size and type of business that, at a minimum, includes each of the requirements of paragraphs (a)(1) through (5) of this section. The CIP must be a part of the...). (2) Identity verification procedures. The CIP must include risk-based procedures for verifying the...

  10. From the patient to the clinical mycology laboratory: how can we optimise microscopy and culture methods for mould identification?

    PubMed

    Vyzantiadis, Timoleon-Achilleas A; Johnson, Elizabeth M; Kibbler, Christopher C

    2012-06-01

    The identification of fungi relies mainly on morphological criteria. However, there is a need for robust and definitive phenotypic identification procedures in order to evaluate continuously evolving molecular methods. For the future, there is an emerging consensus that a combined (phenotypic and molecular) approach is more powerful for fungal identification, especially for moulds. Most of the procedures used for phenotypic identification are based on experience rather than comparative studies of effectiveness or performance and there is a need for standardisation among mycology laboratories. This review summarises and evaluates the evidence for the major existing phenotypic identification procedures for the predominant causes of opportunistic mould infection. We have concentrated mainly on Aspergillus, Fusarium and mucoraceous mould species, as these are the most important clinically and the ones for which there are the most molecular taxonomic data.

  11. Implementation options for DNA-based identification into ecological status assessment under the European Water Framework Directive.

    PubMed

    Hering, Daniel; Borja, Angel; Jones, J Iwan; Pont, Didier; Boets, Pieter; Bouchez, Agnes; Bruce, Kat; Drakare, Stina; Hänfling, Bernd; Kahlert, Maria; Leese, Florian; Meissner, Kristian; Mergen, Patricia; Reyjol, Yorick; Segurado, Pedro; Vogler, Alfried; Kelly, Martyn

    2018-07-01

    Assessment of ecological status for the European Water Framework Directive (WFD) is based on "Biological Quality Elements" (BQEs), namely phytoplankton, benthic flora, benthic invertebrates and fish. Morphological identification of these organisms is a time-consuming and expensive procedure. Here, we assess the options for complementing and, perhaps, replacing morphological identification with procedures using eDNA, metabarcoding or similar approaches. We rate the applicability of DNA-based identification for the individual BQEs and water categories (rivers, lakes, transitional and coastal waters) against eleven criteria, summarised under the headlines representativeness (for example suitability of current sampling methods for DNA-based identification, errors from DNA-based species detection), sensitivity (for example capability to detect sensitive taxa, unassigned reads), precision of DNA-based identification (knowledge about uncertainty), comparability with conventional approaches (for example sensitivity of metrics to differences in DNA-based identification), cost effectiveness and environmental impact. Overall, suitability of DNA-based identification is particularly high for fish, as eDNA is a well-suited sampling approach which can replace expensive and potentially harmful methods such as gill-netting, trawling or electrofishing. Furthermore, there are attempts to replace absolute by relative abundance in metric calculations. For invertebrates and phytobenthos, the main challenges include the modification of indices and completing barcode libraries. For phytoplankton, the barcode libraries are even more problematic, due to the high taxonomic diversity in plankton samples. If current assessment concepts are kept, DNA-based identification is least appropriate for macrophytes (rivers, lakes) and angiosperms/macroalgae (transitional and coastal waters), which are surveyed rather than sampled. We discuss general implications of implementing DNA-based identification into standard ecological assessment, in particular considering any adaptations to the WFD that may be required to facilitate the transition to molecular data. Copyright © 2018 Elsevier Ltd. All rights reserved.

  12. Automated identification of brain tumors from single MR images based on segmentation with refined patient-specific priors

    PubMed Central

    Sanjuán, Ana; Price, Cathy J.; Mancini, Laura; Josse, Goulven; Grogan, Alice; Yamamoto, Adam K.; Geva, Sharon; Leff, Alex P.; Yousry, Tarek A.; Seghier, Mohamed L.

    2013-01-01

    Brain tumors can have different shapes or locations, making their identification very challenging. In functional MRI, it is not unusual that patients have only one anatomical image due to time and financial constraints. Here, we provide a modified automatic lesion identification (ALI) procedure which enables brain tumor identification from single MR images. Our method rests on (A) a modified segmentation-normalization procedure with an explicit “extra prior” for the tumor and (B) an outlier detection procedure for abnormal voxel (i.e., tumor) classification. To minimize tissue misclassification, the segmentation-normalization procedure requires prior information of the tumor location and extent. We therefore propose that ALI is run iteratively so that the output of Step B is used as a patient-specific prior in Step A. We test this procedure on real T1-weighted images from 18 patients, and the results were validated in comparison to two independent observers' manual tracings. The automated procedure identified the tumors successfully with an excellent agreement with the manual segmentation (area under the ROC curve = 0.97 ± 0.03). The proposed procedure increases the flexibility and robustness of the ALI tool and will be particularly useful for lesion-behavior mapping studies, or when lesion identification and/or spatial normalization are problematic. PMID:24381535

  13. Project START: Using a Multiple Intelligences Model in Identifying and Promoting Talent in High-Risk Students. Research Monograph 95136.

    ERIC Educational Resources Information Center

    Callahan, Carolyn M.; Tomlinson, Carol A.; Moon, Tonya R.; Tomchin, Ellen M.; Plucker, Jonathan A.

    This monograph describes Project START (Support To Affirm Rising Talent), a three-year collaborative research effort to develop and apply gifted identification procedures based on Howard Gardner's (1983) theory of multiple intelligences. Specifically, the study attempted to: (1) develop identification procedures; (2) identify high-potential…

  14. IMBLMS phase B4, additional tasks 5.0. Microbial identification system

    NASA Technical Reports Server (NTRS)

    1971-01-01

    A laboratory study was undertaken to provide simplified procedures leading to the presumptive identification (I/D) of defined microorganisms on-board an orbiting spacecraft. Identifications were to be initiated by nonprofessional bacteriologists, (crew members) on a contingency basis only. Key objectives/constraints for this investigation were as follows:(1) I/D procedures based on limited, defined diagnostic tests, (2) testing oriented about ten selected microorganisms, (3) provide for definitive I/D key and procedures per selected organism, (4) define possible occurrences of false positives for the resulting I/D key by search of the appropriate literature, and (5) evaluation of the I/D key and procedure through a limited field trial on randomly selected subjects using the I/D key.

  15. Symbolic dynamic filtering and language measure for behavior identification of mobile robots.

    PubMed

    Mallapragada, Goutham; Ray, Asok; Jin, Xin

    2012-06-01

    This paper presents a procedure for behavior identification of mobile robots, which requires limited or no domain knowledge of the underlying process. While the features of robot behavior are extracted by symbolic dynamic filtering of the observed time series, the behavior patterns are classified based on language measure theory. The behavior identification procedure has been experimentally validated on a networked robotic test bed by comparison with commonly used tools, namely, principal component analysis for feature extraction and Bayesian risk analysis for pattern classification.

  16. [Evaluation of mass spectrometry: MALDI-TOF MS for fast and reliable yeast identification].

    PubMed

    Relloso, María S; Nievas, Jimena; Fares Taie, Santiago; Farquharson, Victoria; Mujica, María T; Romano, Vanesa; Zarate, Mariela S; Smayevsky, Jorgelina

    2015-01-01

    The matrix-assisted laser desorption/ionization time-of-flight mass spectrometry technique known as MALDI-TOF MS is a tool used for the identification of clinical pathogens by generating a protein spectrum that is unique for a given species. In this study we assessed the identification of clinical yeast isolates by MALDI-TOF MS in a university hospital from Argentina and compared two procedures for protein extraction: a rapid method and a procedure based on the manufacturer's recommendations. A short protein extraction procedure was applied in 100 isolates and the rate of correct identification at genus and species level was 98.0%. In addition, we analyzed 201 isolates, previously identified by conventional methods, using the methodology recommended by the manufacturer and there was 95.38% coincidence in the identification at species level. MALDI TOF MS showed to be a fast, simple and reliable tool for yeast identification. Copyright © 2014 Asociación Argentina de Microbiología. Publicado por Elsevier España, S.L.U. All rights reserved.

  17. Testing a potential alternative to traditional identification procedures: Reaction time-based concealed information test does not work for lineups with cooperative witnesses.

    PubMed

    Sauerland, Melanie; Wolfs, Andrea C F; Crans, Samantha; Verschuere, Bruno

    2017-11-27

    Direct eyewitness identification is widely used, but prone to error. We tested the validity of indirect eyewitness identification decisions using the reaction time-based concealed information test (CIT) for assessing cooperative eyewitnesses' face memory as an alternative to traditional lineup procedures. In a series of five experiments, a total of 401 mock eyewitnesses watched one of 11 different stimulus events that depicted a breach of law. Eyewitness identifications in the CIT were derived from longer reaction times as compared to well-matched foil faces not encountered before. Across the five experiments, the weighted mean effect size d was 0.14 (95% CI 0.08-0.19). The reaction time-based CIT seems unsuited for testing cooperative eyewitnesses' memory for faces. The careful matching of the faces required for a fair lineup or the lack of intent to deceive may have hampered the diagnosticity of the reaction time-based CIT.

  18. 31 CFR 1020.220 - Customer identification programs for banks, savings associations, credit unions, and certain non...

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... written Customer Identification Program (CIP) appropriate for its size and type of business that, at a.... 5318(h), 12 U.S.C. 1818(s), or 12 U.S.C. 1786(q)(1), then the CIP must be a part of the anti-money... directors. (2) Identity verification procedures. The CIP must include risk-based procedures for verifying...

  19. 31 CFR 1020.220 - Customer identification programs for banks, savings associations, credit unions, and certain non...

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... written Customer Identification Program (CIP) appropriate for its size and type of business that, at a.... 5318(h), 12 U.S.C. 1818(s), or 12 U.S.C. 1786(q)(1), then the CIP must be a part of the anti-money... directors. (2) Identity verification procedures. The CIP must include risk-based procedures for verifying...

  20. 31 CFR 1020.220 - Customer identification programs for banks, savings associations, credit unions, and certain non...

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... written Customer Identification Program (CIP) appropriate for its size and type of business that, at a.... 5318(h), 12 U.S.C. 1818(s), or 12 U.S.C. 1786(q)(1), then the CIP must be a part of the anti-money... directors. (2) Identity verification procedures. The CIP must include risk-based procedures for verifying...

  1. 31 CFR 1020.220 - Customer identification programs for banks, savings associations, credit unions, and certain non...

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... written Customer Identification Program (CIP) appropriate for its size and type of business that, at a.... 5318(h), 12 U.S.C. 1818(s), or 12 U.S.C. 1786(q)(1), then the CIP must be a part of the anti-money... directors. (2) Identity verification procedures. The CIP must include risk-based procedures for verifying...

  2. Identification procedure for epistemic uncertainties using inverse fuzzy arithmetic

    NASA Astrophysics Data System (ADS)

    Haag, T.; Herrmann, J.; Hanss, M.

    2010-10-01

    For the mathematical representation of systems with epistemic uncertainties, arising, for example, from simplifications in the modeling procedure, models with fuzzy-valued parameters prove to be a suitable and promising approach. In practice, however, the determination of these parameters turns out to be a non-trivial problem. The identification procedure to appropriately update these parameters on the basis of a reference output (measurement or output of an advanced model) requires the solution of an inverse problem. Against this background, an inverse method for the computation of the fuzzy-valued parameters of a model with epistemic uncertainties is presented. This method stands out due to the fact that it only uses feedforward simulations of the model, based on the transformation method of fuzzy arithmetic, along with the reference output. An inversion of the system equations is not necessary. The advancement of the method presented in this paper consists of the identification of multiple input parameters based on a single reference output or measurement. An optimization is used to solve the resulting underdetermined problems by minimizing the uncertainty of the identified parameters. Regions where the identification procedure is reliable are determined by the computation of a feasibility criterion which is also based on the output data of the transformation method only. For a frequency response function of a mechanical system, this criterion allows a restriction of the identification process to some special range of frequency where its solution can be guaranteed. Finally, the practicability of the method is demonstrated by covering the measured output of a fluid-filled piping system by the corresponding uncertain FE model in a conservative way.

  3. Eyewitness Identification Reforms: Are Suggestiveness-Induced Hits and Guesses True Hits?

    PubMed

    Wells, Gary L; Steblay, Nancy K; Dysart, Jennifer E

    2012-05-01

    Research-based reforms for collecting eyewitness identification evidence (e.g., unbiased pre-lineup instructions, double-blind administration) have been proposed by psychologists and adopted in increasing numbers of jurisdictions across the United States. It is well known that reducing rates of mistaken identifications can also reduce accurate identification rates (hits). But the reforms are largely designed to reduce the suggestiveness of the procedures they are meant to replace. Accordingly, we argue that it is misleading to label any hits obtained because of suggestive procedures as "hits" and then saddle reforms with the charge that they reduce the rate of these illegitimate hits. Eyewitness identification evidence should be based solely on the independent memory of the witness, not aided by biased instructions, cues from lineup administrators, or the use of lineup fillers who make the suspect stand out. Failure to call out these hits as being illegitimate can give solace to those who are motivated to preserve the status quo. © The Author(s) 2012.

  4. Identification of cracks in thick beams with a cracked beam element model

    NASA Astrophysics Data System (ADS)

    Hou, Chuanchuan; Lu, Yong

    2016-12-01

    The effect of a crack on the vibration of a beam is a classical problem, and various models have been proposed, ranging from the basic stiffness reduction method to the more sophisticated model involving formulation based on the additional flexibility due to a crack. However, in the damage identification or finite element model updating applications, it is still common practice to employ a simple stiffness reduction factor to represent a crack in the identification process, whereas the use of a more realistic crack model is rather limited. In this paper, the issues with the simple stiffness reduction method, particularly concerning thick beams, are highlighted along with a review of several other crack models. A robust finite element model updating procedure is then presented for the detection of cracks in beams. The description of the crack parameters is based on the cracked beam flexibility formulated by means of the fracture mechanics, and it takes into consideration of shear deformation and coupling between translational and longitudinal vibrations, and thus is particularly suitable for thick beams. The identification procedure employs a global searching technique using Genetic Algorithms, and there is no restriction on the location, severity and the number of cracks to be identified. The procedure is verified to yield satisfactory identification for practically any configurations of cracks in a beam.

  5. Wavelet-based identification of rotor blades in passage-through-resonance tests

    NASA Astrophysics Data System (ADS)

    Carassale, Luigi; Marrè-Brunenghi, Michela; Patrone, Stefano

    2018-01-01

    Turbine blades are critical components in turbo engines and their design process usually includes experimental tests in order to validate and/or update numerical models. These tests are generally carried out on full-scale rotors having some blades instrumented with strain gauges and usually involve a run-up or a run-down phase. The quantification of damping in these conditions is rather challenging for several reasons. In this work, we show through numerical simulations that the usual identification procedures lead to a systematic overestimation of damping due both to the finite sweep velocity, as well as to the variation of the blade natural frequencies with the rotation speed. To overcome these problems, an identification procedure based on the continuous wavelet transform is proposed and validated through numerical simulation.

  6. Qpais: A Web-Based Expert System for Assistedidentification of Quarantine Stored Insect Pests

    NASA Astrophysics Data System (ADS)

    Huang, Han; Rajotte, Edwin G.; Li, Zhihong; Chen, Ke; Zhang, Shengfang

    Stored insect pests can seriously depredate stored products causing worldwide economic losses. Pests enter countries traveling with transported goods. Inspection and Quarantine activities are essential to prevent the invasion and spread of pests. Identification of quarantine stored insect pests is an important component of the China's Inspection and Quarantine procedure, and it is necessary not only to identify whether the species captured is an invasive species, but determine control procedures for stored insect pests. With the development of information technologies, many expert systems that aid in the identification of agricultural pests have been developed. Expert systems for the identification of quarantine stored insect pests are rare and are mainly developed for stand-alone PCs. This paper describes the development of a web-based expert system for identification of quarantine stored insect pests as part of the China 11th Five-Year National Scientific and Technological Support Project (115 Project). Based on user needs, textual knowledge and images were gathered from the literature and expert interviews. ASP.NET, C# and SQL language were used to program the system. Improvement of identification efficiency and flexibility was achieved using a new inference method called characteristic-select-based spatial distance method. The expert system can assist identifying 150 species of quarantine stored insect pests and provide detailed information for each species. The expert system has also been evaluated using two steps: system testing and identification testing. With a 85% rate of correct identification and high efficiency, the system evaluation shows that this expert system can be used in identification work of quarantine stored insect pests.

  7. Impact of the Parameter Identification of Plastic Potentials on the Finite Element Simulation of Sheet Metal Forming

    NASA Astrophysics Data System (ADS)

    Rabahallah, M.; Bouvier, S.; Balan, T.; Bacroix, B.; Teodosiu, C.

    2007-04-01

    In this work, an implicit, backward Euler time integration scheme is developed for an anisotropic, elastic-plastic model based on strain-rate potentials. The constitutive algorithm includes a sub-stepping procedure to deal with the strong nonlinearity of the plastic potentials when applied to FCC materials. The algorithm is implemented in the static implicit version of the Abaqus finite element code. Several recent plastic potentials have been implemented in this framework. The most accurate potentials require the identification of about twenty material parameters. Both mechanical tests and micromechanical simulations have been used for their identification, for a number of BCC and FCC materials. The impact of the identification procedure on the prediction of ears in cup drawing is investigated.

  8. An Evaluation of Alternative Screening Procedures.

    ERIC Educational Resources Information Center

    Reid, Carol; Romanoff, Brenda; Algozzine, Bob; Udall, Ann

    2000-01-01

    This study compared the Problem Solving Assessment (PSA) procedure, an application based on Gardner's theory of multiple intelligences, with more traditional criteria for the identification of minority students for gifted education programs. Although positive correlations among approaches and intelligences were observed, different groups of…

  9. Electro-thermal battery model identification for automotive applications

    NASA Astrophysics Data System (ADS)

    Hu, Y.; Yurkovich, S.; Guezennec, Y.; Yurkovich, B. J.

    This paper describes a model identification procedure for identifying an electro-thermal model of lithium ion batteries used in automotive applications. The dynamic model structure adopted is based on an equivalent circuit model whose parameters are scheduled on the state-of-charge, temperature, and current direction. Linear spline functions are used as the functional form for the parametric dependence. The model identified in this way is valid inside a large range of temperatures and state-of-charge, so that the resulting model can be used for automotive applications such as on-board estimation of the state-of-charge and state-of-health. The model coefficients are identified using a multiple step genetic algorithm based optimization procedure designed for large scale optimization problems. The validity of the procedure is demonstrated experimentally for an A123 lithium ion iron-phosphate battery.

  10. Identification and Quantitative Analysis of Acetaminophen, Acetylsalicylic Acid, and Caffeine in Commercial Analgesic Tablets by LC-MS

    ERIC Educational Resources Information Center

    Fenk, Christopher J.; Hickman, Nicole M.; Fincke, Melissa A.; Motry, Douglas H.; Lavine, Barry

    2010-01-01

    An undergraduate LC-MS experiment is described for the identification and quantitative determination of acetaminophen, acetylsalicylic acid, and caffeine in commercial analgesic tablets. This inquiry-based experimental procedure requires minimal sample preparation and provides good analytical results. Students are provided sufficient background…

  11. Identification of 11-Nor-delta9-tetrahydrocannabinol-9-carboxylic acid in urine by ion trap GC-MS-MS in the context of doping analysis.

    PubMed

    Caballero, Gerardo M; D'Angelo, Carlos; Fraguío, Mariá Sol; Centurión, Osvaldo Teme

    2004-01-01

    The purpose of this study is to develop a sensitive and specific alternative to current gas chromatography (GC)-mass spectrometry (MS) selected ion monitoring confirmation methods of 11-nor-delta9-tetrahydrocannabinol-9-carboxylic acid (cTHC) in human urine samples, in the context of doping analysis. An identification procedure based on the comparison, among suspicious and control samples, of the relative abundances of cTHC selected product ions obtained by GC-tandem MS in an ion trap is presented. The method complies with the identification criteria for qualitative assays established by sports authorities; the comparison procedure is precise, reproducible, specific, and sensitive, thus indicating that it is fit for the purpose of identification accordingly to World Antidoping Agency requirements.

  12. Metamodel-based inverse method for parameter identification: elastic-plastic damage model

    NASA Astrophysics Data System (ADS)

    Huang, Changwu; El Hami, Abdelkhalak; Radi, Bouchaïb

    2017-04-01

    This article proposed a metamodel-based inverse method for material parameter identification and applies it to elastic-plastic damage model parameter identification. An elastic-plastic damage model is presented and implemented in numerical simulation. The metamodel-based inverse method is proposed in order to overcome the disadvantage in computational cost of the inverse method. In the metamodel-based inverse method, a Kriging metamodel is constructed based on the experimental design in order to model the relationship between material parameters and the objective function values in the inverse problem, and then the optimization procedure is executed by the use of a metamodel. The applications of the presented material model and proposed parameter identification method in the standard A 2017-T4 tensile test prove that the presented elastic-plastic damage model is adequate to describe the material's mechanical behaviour and that the proposed metamodel-based inverse method not only enhances the efficiency of parameter identification but also gives reliable results.

  13. Mock-juror evaluations of traditional and ratings-based eyewitness identification evidence.

    PubMed

    Sauer, James D; Palmer, Matthew A; Brewer, Neil

    2017-08-01

    Compared to categorical identifications, culprit likelihood ratings (having the witness rate, for each lineup member, the likelihood that the individual is the culprit) provide a promising alternative for assessing a suspect's likely guilt. Four experiments addressed 2 broad questions about the use of culprit likelihood ratings evidence by mock-jurors. First, are mock-jurors receptive to noncategorical forms of identification evidence? Second, does the additional information provided by ratings (relating to discrimination) affect jurors' evaluations of the identification evidence? Experiments 1 and 1A manipulated confidence (90% vs. 50%) and discrimination (good, poor, no information) between participants. Evaluations were influenced by confidence, but not discrimination. However, a within-participant manipulation of discrimination (Experiment 2) demonstrated that evidence of good discrimination enhanced the persuasiveness of moderate levels of confidence, while poor discrimination reduced the persuasiveness of high levels of confidence. Thus, participants can interpret ratings-based evidence, but may not intuit the discrimination information when evaluating ratings for a single identification procedure. Providing detailed instructions about interpreting ratings produced clear discrimination effects when evaluating a single identification procedure (Experiment 3). Across 4 experiments, we found no evidence that mock-jurors perceived noncategorical identification evidence to be less informative than categorical evidence. However, jurors will likely benefit from instruction when interpreting ratings provided by a single witness. (PsycINFO Database Record (c) 2017 APA, all rights reserved).

  14. Category identification of changed land-use polygons in an integrated image processing/geographic information system

    NASA Technical Reports Server (NTRS)

    Westmoreland, Sally; Stow, Douglas A.

    1992-01-01

    A framework is proposed for analyzing ancillary data and developing procedures for incorporating ancillary data to aid interactive identification of land-use categories in land-use updates. The procedures were developed for use within an integrated image processsing/geographic information systems (GIS) that permits simultaneous display of digital image data with the vector land-use data to be updated. With such systems and procedures, automated techniques are integrated with visual-based manual interpretation to exploit the capabilities of both. The procedural framework developed was applied as part of a case study to update a portion of the land-use layer in a regional scale GIS. About 75 percent of the area in the study site that experienced a change in land use was correctly labeled into 19 categories using the combination of automated and visual interpretation procedures developed in the study.

  15. On-line identification of fermentation processes for ethanol production.

    PubMed

    Câmara, M M; Soares, R M; Feital, T; Naomi, P; Oki, S; Thevelein, J M; Amaral, M; Pinto, J C

    2017-07-01

    A strategy for monitoring fermentation processes, specifically, simultaneous saccharification and fermentation (SSF) of corn mash, was developed. The strategy covered the development and use of first principles, semimechanistic and unstructured process model based on major kinetic phenomena, along with mass and energy balances. The model was then used as a reference model within an identification procedure capable of running on-line. The on-line identification procedure consists on updating the reference model through the estimation of corrective parameters for certain reaction rates using the most recent process measurements. The strategy makes use of standard laboratory measurements for sugars quantification and in situ temperature and liquid level data. The model, along with the on-line identification procedure, has been tested against real industrial data and have been able to accurately predict the main variables of operational interest, i.e., state variables and its dynamics, and key process indicators. The results demonstrate that the strategy is capable of monitoring, in real time, this complex industrial biomass fermentation. This new tool provides a great support for decision-making and opens a new range of opportunities for industrial optimization.

  16. Identification of treatment responders based on multiple longitudinal outcomes with applications to multiple sclerosis patients.

    PubMed

    Kondo, Yumi; Zhao, Yinshan; Petkau, John

    2017-05-30

    Identification of treatment responders is a challenge in comparative studies where treatment efficacy is measured by multiple longitudinally collected continuous and count outcomes. Existing procedures often identify responders on the basis of only a single outcome. We propose a novel multiple longitudinal outcome mixture model that assumes that, conditionally on a cluster label, each longitudinal outcome is from a generalized linear mixed effect model. We utilize a Monte Carlo expectation-maximization algorithm to obtain the maximum likelihood estimates of our high-dimensional model and classify patients according to their estimated posterior probability of being a responder. We demonstrate the flexibility of our novel procedure on two multiple sclerosis clinical trial datasets with distinct data structures. Our simulation study shows that incorporating multiple outcomes improves the responder identification performance; this can occur even if some of the outcomes are ineffective. Our general procedure facilitates the identification of responders who are comprehensively defined by multiple outcomes from various distributions. Copyright © 2017 John Wiley & Sons, Ltd. Copyright © 2017 John Wiley & Sons, Ltd.

  17. A Framework for Creating a Function-based Design Tool for Failure Mode Identification

    NASA Technical Reports Server (NTRS)

    Arunajadai, Srikesh G.; Stone, Robert B.; Tumer, Irem Y.; Clancy, Daniel (Technical Monitor)

    2002-01-01

    Knowledge of potential failure modes during design is critical for prevention of failures. Currently industries use procedures such as Failure Modes and Effects Analysis (FMEA), Fault Tree analysis, or Failure Modes, Effects and Criticality analysis (FMECA), as well as knowledge and experience, to determine potential failure modes. When new products are being developed there is often a lack of sufficient knowledge of potential failure mode and/or a lack of sufficient experience to identify all failure modes. This gives rise to a situation in which engineers are unable to extract maximum benefits from the above procedures. This work describes a function-based failure identification methodology, which would act as a storehouse of information and experience, providing useful information about the potential failure modes for the design under consideration, as well as enhancing the usefulness of procedures like FMEA. As an example, the method is applied to fifteen products and the benefits are illustrated.

  18. The (Un)Certainty of Selectivity in Liquid Chromatography Tandem Mass Spectrometry

    NASA Astrophysics Data System (ADS)

    Berendsen, Bjorn J. A.; Stolker, Linda A. M.; Nielen, Michel W. F.

    2013-01-01

    We developed a procedure to determine the "identification power" of an LC-MS/MS method operated in the MRM acquisition mode, which is related to its selectivity. The probability of any compound showing the same precursor ion, product ions, and retention time as the compound of interest is used as a measure of selectivity. This is calculated based upon empirical models constructed from three very large compound databases. Based upon the final probability estimation, additional measures to assure unambiguous identification can be taken, like the selection of different or additional product ions. The reported procedure in combination with criteria for relative ion abundances results in a powerful technique to determine the (un)certainty of the selectivity of any LC-MS/MS analysis and thus the risk of false positive results. Furthermore, the procedure is very useful as a tool to validate method selectivity.

  19. Identifying Adolescents at Risk through Voluntary School-Based Mental Health Screening

    ERIC Educational Resources Information Center

    Husky, Mathilde M.; Kaplan, Adam; McGuire, Leslie; Flynn, Laurie; Chrostowski, Christine; Olfson, Mark

    2011-01-01

    This study compares referrals for mental health services among high school students randomized to two means of referral to mental health services: referral via systematic identification through a brief mental health screening procedure (n = 365) or referral via the usual process of identification by school personnel, parents, or students…

  20. A rapid, one step molecular identification of Trichoderma citrinoviride and Trichoderma reesei.

    PubMed

    Saroj, Dina B; Dengeti, Shrinivas N; Aher, Supriya; Gupta, Anil K

    2015-06-01

    Trichoderma species are widely used as production hosts for industrial enzymes. Identification of Trichoderma species requires a complex molecular biology based identification involving amplification and sequencing of multiple genes. Industrial laboratories are required to run identification tests repeatedly in cell banking procedures and also to prove absence of production host in the product. Such demands can be fulfilled by a brief method which enables confirmation of strain identity. This communication describes one step identification method for two common Trichoderma species; T. citrinoviride and T. reesei, based on identification of polymorphic region in the nucleotide sequence of translation elongation factor 1 alpha. A unique forward primer and common reverse primer resulted in 153 and 139 bp amplicon for T. citrinoviride and T. reesei, respectively. Simplification was further introduced by using mycelium as template for PCR amplification. Method described in this communication allows rapid, one step identification of two Trichoderma species.

  1. Development of techniques for producing static strata maps and development of photointerpretation methods based on multitemporal LANDSAT data

    NASA Technical Reports Server (NTRS)

    Colwell, R. N. (Principal Investigator); Hay, C. M.; Thomas, R. W.; Benson, A. S.

    1976-01-01

    The progress of research conducted in support of the Large Area Crop Inventory Experiment (LACIE) is documented. Specific tasks include (1) evaluation of the static stratification procedure and modification of that procedure if warranted, and (2) the development of alternative photointerpretative techniques to the present LACIE procedures for the identification and selection of training fields (areas).

  2. Development of techniques for producing static strata maps and development of photointerpretive methods based on multitemporal LANDSAT data

    NASA Technical Reports Server (NTRS)

    Colwell, R. N. (Principal Investigator); Hay, C. M.; Thomas, R. W.; Benson, A. S.

    1977-01-01

    Progress in the evaluation of the static stratification procedure and the development of alternative photointerpretive techniques to the present LACIE procedure for the identification of training fields is reported. Statistically significant signature controlling variables were defined for use in refining the stratification procedure. A subset of the 1973-74 Kansas LACIE segments for wheat was analyzed.

  3. Probability of identification: a statistical model for the validation of qualitative botanical identification methods.

    PubMed

    LaBudde, Robert A; Harnly, James M

    2012-01-01

    A qualitative botanical identification method (BIM) is an analytical procedure that returns a binary result (1 = Identified, 0 = Not Identified). A BIM may be used by a buyer, manufacturer, or regulator to determine whether a botanical material being tested is the same as the target (desired) material, or whether it contains excessive nontarget (undesirable) material. The report describes the development and validation of studies for a BIM based on the proportion of replicates identified, or probability of identification (POI), as the basic observed statistic. The statistical procedures proposed for data analysis follow closely those of the probability of detection, and harmonize the statistical concepts and parameters between quantitative and qualitative method validation. Use of POI statistics also harmonizes statistical concepts for botanical, microbiological, toxin, and other analyte identification methods that produce binary results. The POI statistical model provides a tool for graphical representation of response curves for qualitative methods, reporting of descriptive statistics, and application of performance requirements. Single collaborator and multicollaborative study examples are given.

  4. 14 CFR 1212.202 - Identification procedures.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 14 Aeronautics and Space 5 2011-01-01 2010-01-01 true Identification procedures. 1212.202 Section 1212.202 Aeronautics and Space NATIONAL AERONAUTICS AND SPACE ADMINISTRATION PRIVACY ACT-NASA REGULATIONS Access to Records § 1212.202 Identification procedures. (a) The system manager will release...

  5. 14 CFR 1212.202 - Identification procedures.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 14 Aeronautics and Space 5 2010-01-01 2010-01-01 false Identification procedures. 1212.202 Section 1212.202 Aeronautics and Space NATIONAL AERONAUTICS AND SPACE ADMINISTRATION PRIVACY ACT-NASA REGULATIONS Access to Records § 1212.202 Identification procedures. (a) The system manager will release...

  6. Viewing videotaped identification procedure increases juror sensitivity to single-blind photo-array administration.

    PubMed

    Modjadidi, Karima; Kovera, Margaret Bull

    2018-06-01

    We investigated whether watching a videotaped photo array administration or expert testimony could sensitize jurors to the suggestiveness of single-blind eyewitness identification procedures. Mock jurors recruited from the community (N = 231) watched a videotaped simulation of a robbery trial in which the primary evidence against the defendant was an eyewitness identification. We varied whether the witness made an identification from a single- or double-blind photo array, the evidence included a videotape of the photo array procedure, and an expert testified about the effects of single-blind identification procedures on administrators' behaviors and witness accuracy. Watching the videotaped photo array administration sensitized mock jurors to the suggestiveness of the single-blind procedure, causing them to be less likely to convict a defendant identified through single-rather than double-blind procedures. Exposure to the videotaped procedure also decreased the favorability of mock jurors' ratings of the eyewitness, irrespective of whether the lineup was conducted by a single-blind administrator. Expert testimony did not sensitize jurors to administrator bias. Thus, videotaping identification procedures could serve as an important procedural reform that both preserves a record of whether the lineup administration was suggestive and might improve jurors' evaluations of eyewitness evidence. (PsycINFO Database Record (c) 2018 APA, all rights reserved).

  7. Field experiments on eyewitness identification: towards a better understanding of pitfalls and prospects.

    PubMed

    Wells, Gary L

    2008-02-01

    The Illinois pilot program on lineup procedures has helped sharpen the focus on the types of controls that are needed in eyewitness field experiments and the limits that exist for interpreting outcome measures (rates of suspect and filler identifications). A widely-known limitation of field experiments is that, unlike simulated crime experiments, the guilt or innocence of the suspects is not easily known independently of the behavior of the eyewitnesses. Less well appreciated is that the rate of identification of lineup fillers, although clearly errors, can be a misleading measure if the filler identification rate is used to assess which of two or more lineup procedures is the better procedure. Several examples are used to illustrate that there are clearly improper procedures that would yield fewer identifications of fillers than would their proper counterparts. For example, biased lineup structure (e.g., using poorly matched fillers) as well as suggestive lineup procedures (that can result from non-blind administration of lineups) would reduce filler identification errors compared to unbiased and non-suggestive procedures. Hence, under many circumstances filler identification rates can be misleading indicators of preferred methods. Comparisons of lineup procedures in future field experiments will not be easily accepted in the absence of double-blind administration methods in all conditions plus true random assignment to conditions.

  8. Identification of microbes from the surfaces of food-processing lines based on the flow cytometric evaluation of cellular metabolic activity combined with cell sorting.

    PubMed

    Juzwa, W; Duber, A; Myszka, K; Białas, W; Czaczyk, K

    2016-09-01

    In this study the design of a flow cytometry-based procedure to facilitate the detection of adherent bacteria from food-processing surfaces was evaluated. The measurement of the cellular redox potential (CRP) of microbial cells was combined with cell sorting for the identification of microorganisms. The procedure enhanced live/dead cell discrimination owing to the measurement of the cell physiology. The microbial contamination of the surface of a stainless steel conveyor used to process button mushrooms was evaluated in three independent experiments. The flow cytometry procedure provided a step towards monitoring of contamination and enabled the assessment of microbial food safety hazards by the discrimination of active, mid-active and non-active bacterial sub-populations based on determination of their cellular vitality and subsequently single cell sorting to isolate microbial strains from discriminated sub-populations. There was a significant correlation (r = 0.97; p < 0.05) between the bacterial cell count estimated by the pour plate method and flow cytometry, despite there being differences in the absolute number of cells detected. The combined approach of flow cytometric CRP measurement and cell sorting allowed an in situ analysis of microbial cell vitality and the identification of species from defined sub-populations, although the identified microbes were limited to culturable cells.

  9. Aeroservoelastic Model Validation and Test Data Analysis of the F/A-18 Active Aeroelastic Wing

    NASA Technical Reports Server (NTRS)

    Brenner, Martin J.; Prazenica, Richard J.

    2003-01-01

    Model validation and flight test data analysis require careful consideration of the effects of uncertainty, noise, and nonlinearity. Uncertainty prevails in the data analysis techniques and results in a composite model uncertainty from unmodeled dynamics, assumptions and mechanics of the estimation procedures, noise, and nonlinearity. A fundamental requirement for reliable and robust model development is an attempt to account for each of these sources of error, in particular, for model validation, robust stability prediction, and flight control system development. This paper is concerned with data processing procedures for uncertainty reduction in model validation for stability estimation and nonlinear identification. F/A-18 Active Aeroelastic Wing (AAW) aircraft data is used to demonstrate signal representation effects on uncertain model development, stability estimation, and nonlinear identification. Data is decomposed using adaptive orthonormal best-basis and wavelet-basis signal decompositions for signal denoising into linear and nonlinear identification algorithms. Nonlinear identification from a wavelet-based Volterra kernel procedure is used to extract nonlinear dynamics from aeroelastic responses, and to assist model development and uncertainty reduction for model validation and stability prediction by removing a class of nonlinearity from the uncertainty.

  10. Jersey number detection in sports video for athlete identification

    NASA Astrophysics Data System (ADS)

    Ye, Qixiang; Huang, Qingming; Jiang, Shuqiang; Liu, Yang; Gao, Wen

    2005-07-01

    Athlete identification is important for sport video content analysis since users often care about the video clips with their preferred athletes. In this paper, we propose a method for athlete identification by combing the segmentation, tracking and recognition procedures into a coarse-to-fine scheme for jersey number (digital characters on sport shirt) detection. Firstly, image segmentation is employed to separate the jersey number regions with its background. And size/pipe-like attributes of digital characters are used to filter out candidates. Then, a K-NN (K nearest neighbor) classifier is employed to classify a candidate into a digit in "0-9" or negative. In the recognition procedure, we use the Zernike moment features, which are invariant to rotation and scale for digital shape recognition. Synthetic training samples with different fonts are used to represent the pattern of digital characters with non-rigid deformation. Once a character candidate is detected, a SSD (smallest square distance)-based tracking procedure is started. The recognition procedure is performed every several frames in the tracking process. After tracking tens of frames, the overall recognition results are combined to determine if a candidate is a true jersey number or not by a voting procedure. Experiments on several types of sports video shows encouraging result.

  11. Identification of Bouc-Wen hysteretic parameters based on enhanced response sensitivity approach

    NASA Astrophysics Data System (ADS)

    Wang, Li; Lu, Zhong-Rong

    2017-05-01

    This paper aims to identify parameters of Bouc-Wen hysteretic model using time-domain measured data. It follows a general inverse identification procedure, that is, identifying model parameters is treated as an optimization problem with the nonlinear least squares objective function. Then, the enhanced response sensitivity approach, which has been shown convergent and proper for such kind of problems, is adopted to solve the optimization problem. Numerical tests are undertaken to verify the proposed identification approach.

  12. Identification and calibration of the structural model of historical masonry building damaged during the 2016 Italian earthquakes: The case study of Palazzo del Podestà in Montelupone

    NASA Astrophysics Data System (ADS)

    Catinari, Federico; Pierdicca, Alessio; Clementi, Francesco; Lenci, Stefano

    2017-11-01

    The results of an ambient-vibration based investigation conducted on the "Palazzo del Podesta" in Montelupone (Italy) is presented. The case study was damaged during the 20I6 Italian earthquakes that stroke the central part of the Italy. The assessment procedure includes full-scale ambient vibration testing, modal identification from ambient vibration responses, finite element modeling and dynamic-based identification of the uncertain structural parameters of the model. A very good match between theoretical and experimental modal parameters was reached and the model updating has been performed identifying some structural parameters.

  13. Eyewitness Identification Reform: Data, Theory, and Due Process.

    PubMed

    Clark, Steven E

    2012-05-01

    Some commentators view my analyses (Clark, 2012, this issue) as an important step forward in assessing the costs and benefits of eyewitness identification reform. Others suggest that the trade-off between correct identifications lost and false identifications avoided is well-known; that the expected utility model is misspecified; and that the loss of correct identifications due to the use of reformed eyewitness identification procedures is irrelevant to policy decisions, as those correct identifications are the illegitimate product of suggestion and lucky guesses. Contrary to these criticisms, the loss of correct identifications has not been adequately considered in theoretical or policy matters, criticisms regarding the various utilities do not substantively change the nature of the trade-off, and the dismissal of lost correct identifications is based not on data but on an outdated theory of recognition memory. © The Author(s) 2012.

  14. DNA Barcoding of Sigmodontine Rodents: Identifying Wildlife Reservoirs of Zoonoses

    PubMed Central

    Müller, Lívia; Gonçalves, Gislene L.; Cordeiro-Estrela, Pedro; Marinho, Jorge R.; Althoff, Sérgio L.; Testoni, André. F.; González, Enrique M.; Freitas, Thales R. O.

    2013-01-01

    Species identification through DNA barcoding is a tool to be added to taxonomic procedures, once it has been validated. Applying barcoding techniques in public health would aid in the identification and correct delimitation of the distribution of rodents from the subfamily Sigmodontinae. These rodents are reservoirs of etiological agents of zoonoses including arenaviruses, hantaviruses, Chagas disease and leishmaniasis. In this study we compared distance-based and probabilistic phylogenetic inference methods to evaluate the performance of cytochrome c oxidase subunit I (COI) in sigmodontine identification. A total of 130 sequences from 21 field-trapped species (13 genera), mainly from southern Brazil, were generated and analyzed, together with 58 GenBank sequences (24 species; 10 genera). Preliminary analysis revealed a 9.5% rate of misidentifications in the field, mainly of juveniles, which were reclassified after examination of external morphological characters and chromosome numbers. Distance and model-based methods of tree reconstruction retrieved similar topologies and monophyly for most species. Kernel density estimation of the distance distribution showed a clear barcoding gap with overlapping of intraspecific and interspecific densities < 1% and 21 species with mean intraspecific distance < 2%. Five species that are reservoirs of hantaviruses could be identified through DNA barcodes. Additionally, we provide information for the description of a putative new species, as well as the first COI sequence of the recently described genus Drymoreomys. The data also indicated an expansion of the distribution of Calomys tener. We emphasize that DNA barcoding should be used in combination with other taxonomic and systematic procedures in an integrative framework and based on properly identified museum collections, to improve identification procedures, especially in epidemiological surveillance and ecological assessments. PMID:24244670

  15. A hybrid approach to parameter identification of linear delay differential equations involving multiple delays

    NASA Astrophysics Data System (ADS)

    Marzban, Hamid Reza

    2018-05-01

    In this paper, we are concerned with the parameter identification of linear time-invariant systems containing multiple delays. The approach is based upon a hybrid of block-pulse functions and Legendre's polynomials. The convergence of the proposed procedure is established and an upper error bound with respect to the L2-norm associated with the hybrid functions is derived. The problem under consideration is first transformed into a system of algebraic equations. The least squares technique is then employed for identification of the desired parameters. Several multi-delay systems of varying complexity are investigated to evaluate the performance and capability of the proposed approximation method. It is shown that the proposed approach is also applicable to a class of nonlinear multi-delay systems. It is demonstrated that the suggested procedure provides accurate results for the desired parameters.

  16. Estimating the reliability of eyewitness identifications from police lineups

    PubMed Central

    Wixted, John T.; Mickes, Laura; Dunn, John C.; Clark, Steven E.; Wells, William

    2016-01-01

    Laboratory-based mock crime studies have often been interpreted to mean that (i) eyewitness confidence in an identification made from a lineup is a weak indicator of accuracy and (ii) sequential lineups are diagnostically superior to traditional simultaneous lineups. Largely as a result, juries are increasingly encouraged to disregard eyewitness confidence, and up to 30% of law enforcement agencies in the United States have adopted the sequential procedure. We conducted a field study of actual eyewitnesses who were assigned to simultaneous or sequential photo lineups in the Houston Police Department over a 1-y period. Identifications were made using a three-point confidence scale, and a signal detection model was used to analyze and interpret the results. Our findings suggest that (i) confidence in an eyewitness identification from a fair lineup is a highly reliable indicator of accuracy and (ii) if there is any difference in diagnostic accuracy between the two lineup formats, it likely favors the simultaneous procedure. PMID:26699467

  17. Estimating the reliability of eyewitness identifications from police lineups.

    PubMed

    Wixted, John T; Mickes, Laura; Dunn, John C; Clark, Steven E; Wells, William

    2016-01-12

    Laboratory-based mock crime studies have often been interpreted to mean that (i) eyewitness confidence in an identification made from a lineup is a weak indicator of accuracy and (ii) sequential lineups are diagnostically superior to traditional simultaneous lineups. Largely as a result, juries are increasingly encouraged to disregard eyewitness confidence, and up to 30% of law enforcement agencies in the United States have adopted the sequential procedure. We conducted a field study of actual eyewitnesses who were assigned to simultaneous or sequential photo lineups in the Houston Police Department over a 1-y period. Identifications were made using a three-point confidence scale, and a signal detection model was used to analyze and interpret the results. Our findings suggest that (i) confidence in an eyewitness identification from a fair lineup is a highly reliable indicator of accuracy and (ii) if there is any difference in diagnostic accuracy between the two lineup formats, it likely favors the simultaneous procedure.

  18. Development of an Identification Procedure for a Large Urban School Corporation: "Identifying Culturally Diverse and Academically Gifted Elementary Students"

    ERIC Educational Resources Information Center

    Pierce, Rebecca L.; Adams, Cheryll M.; Neumeister, Kristie L. Speirs; Cassady, Jerrell C.; Dixon, Felicia A.; Cross, Tracy L.

    2006-01-01

    This paper describes the identification process of a Priority One Jacob K. Javits grant, Clustering Learners Unlocks Equity (Project CLUE), a university-school partnership. Project CLUE uses a "sift-down model" to cast the net widely as the talent pool of gifted second-grade students is formed. The model is based on standardized test scores, a…

  19. Federal Logistics Information System. FLIS Procedures Manual Publications. Volume 15.

    DTIC Science & Technology

    1995-01-01

    which provides for the processing of adjustments/revisions to established item identifications and characteristics in the FLIS Data Base. Item Logistics...A function in FLIS which provides for the processing of adjustments/revisions to established item identifications and characteristics in the FLIS...the materiel management functions for assigned items. Mechanization of Warehousing and Shipment Processing (MOWASP). A uniform data 6 system designed

  20. Lesion identification using unified segmentation-normalisation models and fuzzy clustering

    PubMed Central

    Seghier, Mohamed L.; Ramlackhansingh, Anil; Crinion, Jenny; Leff, Alexander P.; Price, Cathy J.

    2008-01-01

    In this paper, we propose a new automated procedure for lesion identification from single images based on the detection of outlier voxels. We demonstrate the utility of this procedure using artificial and real lesions. The scheme rests on two innovations: First, we augment the generative model used for combined segmentation and normalization of images, with an empirical prior for an atypical tissue class, which can be optimised iteratively. Second, we adopt a fuzzy clustering procedure to identify outlier voxels in normalised gray and white matter segments. These two advances suppress misclassification of voxels and restrict lesion identification to gray/white matter lesions respectively. Our analyses show a high sensitivity for detecting and delineating brain lesions with different sizes, locations, and textures. Our approach has important implications for the generation of lesion overlap maps of a given population and the assessment of lesion-deficit mappings. From a clinical perspective, our method should help to compute the total volume of lesion or to trace precisely lesion boundaries that might be pertinent for surgical or diagnostic purposes. PMID:18482850

  1. Rapid label-free identification of Klebsiella pneumoniae antibiotic resistant strains by the drop-coating deposition surface-enhanced Raman scattering method

    NASA Astrophysics Data System (ADS)

    Cheong, Youjin; Kim, Young Jin; Kang, Heeyoon; Choi, Samjin; Lee, Hee Joo

    2017-08-01

    Although many methodologies have been developed to identify unknown bacteria, bacterial identification in clinical microbiology remains a complex and time-consuming procedure. To address this problem, we developed a label-free method for rapidly identifying clinically relevant multilocus sequencing typing-verified quinolone-resistant Klebsiella pneumoniae strains. We also applied the method to identify three strains from colony samples, ATCC70063 (control), ST11 and ST15; these are the prevalent quinolone-resistant K. pneumoniae strains in East Asia. The colonies were identified using a drop-coating deposition surface-enhanced Raman scattering (DCD-SERS) procedure coupled with a multivariate statistical method. Our workflow exhibited an enhancement factor of 11.3 × 106 to Raman intensities, high reproducibility (relative standard deviation of 7.4%), and a sensitive limit of detection (100 pM rhodamine 6G), with a correlation coefficient of 0.98. All quinolone-resistant K. pneumoniae strains showed similar spectral Raman shifts (high correlations) regardless of bacterial type, as well as different Raman vibrational modes compared to Escherichia coli strains. Our proposed DCD-SERS procedure coupled with the multivariate statistics-based identification method achieved excellent performance in discriminating similar microbes from one another and also in subtyping of K. pneumoniae strains. Therefore, our label-free DCD-SERS procedure coupled with the computational decision supporting method is a potentially useful method for the rapid identification of clinically relevant K. pneumoniae strains.

  2. A Comprehensive Strategy to Construct In-house Database for Accurate and Batch Identification of Small Molecular Metabolites.

    PubMed

    Zhao, Xinjie; Zeng, Zhongda; Chen, Aiming; Lu, Xin; Zhao, Chunxia; Hu, Chunxiu; Zhou, Lina; Liu, Xinyu; Wang, Xiaolin; Hou, Xiaoli; Ye, Yaorui; Xu, Guowang

    2018-05-29

    Identification of the metabolites is an essential step in metabolomics study to interpret regulatory mechanism of pathological and physiological processes. However, it is still a big headache in LC-MSn-based studies because of the complexity of mass spectrometry, chemical diversity of metabolites, and deficiency of standards database. In this work, a comprehensive strategy is developed for accurate and batch metabolite identification in non-targeted metabolomics studies. First, a well defined procedure was applied to generate reliable and standard LC-MS2 data including tR, MS1 and MS2 information at a standard operational procedure (SOP). An in-house database including about 2000 metabolites was constructed and used to identify the metabolites in non-targeted metabolic profiling by retention time calibration using internal standards, precursor ion alignment and ion fusion, auto-MS2 information extraction and selection, and database batch searching and scoring. As an application example, a pooled serum sample was analyzed to deliver the strategy, 202 metabolites were identified in the positive ion mode. It shows our strategy is useful for LC-MSn-based non-targeted metabolomics study.

  3. An adaptive deep learning approach for PPG-based identification.

    PubMed

    Jindal, V; Birjandtalab, J; Pouyan, M Baran; Nourani, M

    2016-08-01

    Wearable biosensors have become increasingly popular in healthcare due to their capabilities for low cost and long term biosignal monitoring. This paper presents a novel two-stage technique to offer biometric identification using these biosensors through Deep Belief Networks and Restricted Boltzman Machines. Our identification approach improves robustness in current monitoring procedures within clinical, e-health and fitness environments using Photoplethysmography (PPG) signals through deep learning classification models. The approach is tested on TROIKA dataset using 10-fold cross validation and achieved an accuracy of 96.1%.

  4. Use of MALDI-TOF Mass Spectrometry and a Custom Database to Characterize Bacteria Indigenous to a Unique Cave Environment (Kartchner Caverns, AZ, USA)

    PubMed Central

    Zhang, Lin; Vranckx, Katleen; Janssens, Koen; Sandrin, Todd R.

    2015-01-01

    MALDI-TOF mass spectrometry has been shown to be a rapid and reliable tool for identification of bacteria at the genus and species, and in some cases, strain levels. Commercially available and open source software tools have been developed to facilitate identification; however, no universal/standardized data analysis pipeline has been described in the literature. Here, we provide a comprehensive and detailed demonstration of bacterial identification procedures using a MALDI-TOF mass spectrometer. Mass spectra were collected from 15 diverse bacteria isolated from Kartchner Caverns, AZ, USA, and identified by 16S rDNA sequencing. Databases were constructed in BioNumerics 7.1. Follow-up analyses of mass spectra were performed, including cluster analyses, peak matching, and statistical analyses. Identification was performed using blind-coded samples randomly selected from these 15 bacteria. Two identification methods are presented: similarity coefficient-based and biomarker-based methods. Results show that both identification methods can identify the bacteria to the species level. PMID:25590854

  5. Use of MALDI-TOF mass spectrometry and a custom database to characterize bacteria indigenous to a unique cave environment (Kartchner Caverns, AZ, USA).

    PubMed

    Zhang, Lin; Vranckx, Katleen; Janssens, Koen; Sandrin, Todd R

    2015-01-02

    MALDI-TOF mass spectrometry has been shown to be a rapid and reliable tool for identification of bacteria at the genus and species, and in some cases, strain levels. Commercially available and open source software tools have been developed to facilitate identification; however, no universal/standardized data analysis pipeline has been described in the literature. Here, we provide a comprehensive and detailed demonstration of bacterial identification procedures using a MALDI-TOF mass spectrometer. Mass spectra were collected from 15 diverse bacteria isolated from Kartchner Caverns, AZ, USA, and identified by 16S rDNA sequencing. Databases were constructed in BioNumerics 7.1. Follow-up analyses of mass spectra were performed, including cluster analyses, peak matching, and statistical analyses. Identification was performed using blind-coded samples randomly selected from these 15 bacteria. Two identification methods are presented: similarity coefficient-based and biomarker-based methods. Results show that both identification methods can identify the bacteria to the species level.

  6. The effect on cadaver blood DNA identification by the use of targeted and whole body post-mortem computed tomography angiography.

    PubMed

    Rutty, Guy N; Barber, Jade; Amoroso, Jasmin; Morgan, Bruno; Graham, Eleanor A M

    2013-12-01

    Post-mortem computed tomography angiography (PMCTA) involves the injection of contrast agents. This could have both a dilution effect on biological fluid samples and could affect subsequent post-contrast analytical laboratory processes. We undertook a small sample study of 10 targeted and 10 whole body PMCTA cases to consider whether or not these two methods of PMCTA could affect post-PMCTA cadaver blood based DNA identification. We used standard methodology to examine DNA from blood samples obtained before and after the PMCTA procedure. We illustrate that neither of these PMCTA methods had an effect on the alleles called following short tandem repeat based DNA profiling, and therefore the ability to undertake post-PMCTA blood based DNA identification.

  7. Experimental Identification and Characterization of Multirotor UAV Propulsion

    NASA Astrophysics Data System (ADS)

    Kotarski, Denis; Krznar, Matija; Piljek, Petar; Simunic, Nikola

    2017-07-01

    In this paper, an experimental procedure for the identification and characterization of multirotor Unmanned Aerial Vehicle (UAV) propulsion is presented. Propulsion configuration needs to be defined precisely in order to achieve required flight performance. Based on the accurate dynamic model and empirical measurements of multirotor propulsion physical parameters, it is possible to design diverse configurations with different characteristics for various purposes. As a case study, we investigated design considerations for a micro indoor multirotor which is suitable for control algorithm implementation in structured environment. It consists of open source autopilot, sensors for indoor flight, “take off the shelf” propulsion components and frame. The series of experiments were conducted to show the process of parameters identification and the procedure for analysis and propulsion characterization. Additionally, we explore battery performance in terms of mass and specific energy. Experimental results show identified and estimated propulsion parameters through which blade element theory is verified.

  8. Development of techniques for producing static strata maps and development of photointerpretive methods based on multitemporal LANDSAT data

    NASA Technical Reports Server (NTRS)

    Colwell, R. N. (Principal Investigator)

    1977-01-01

    The results and progress of work conducted in support of the Large Area Crop Inventory Experiment (LACIE) are documented. Research was conducted for two tasks. These tasks include: (1) evaluation of the UCB static stratification procedure and modification of that procedure if warranted; and (2) the development of alternative photointerpretive techniques to the present LACIE procedure for the identification and selection of training areas for machine-processing of LACIE segments.

  9. Toward Worldwide Hepcidin Assay Harmonization: Identification of a Commutable Secondary Reference Material.

    PubMed

    van der Vorm, Lisa N; Hendriks, Jan C M; Laarakkers, Coby M; Klaver, Siem; Armitage, Andrew E; Bamberg, Alison; Geurts-Moespot, Anneke J; Girelli, Domenico; Herkert, Matthias; Itkonen, Outi; Konrad, Robert J; Tomosugi, Naohisa; Westerman, Mark; Bansal, Sukhvinder S; Campostrini, Natascia; Drakesmith, Hal; Fillet, Marianne; Olbina, Gordana; Pasricha, Sant-Rayn; Pitts, Kelly R; Sloan, John H; Tagliaro, Franco; Weykamp, Cas W; Swinkels, Dorine W

    2016-07-01

    Absolute plasma hepcidin concentrations measured by various procedures differ substantially, complicating interpretation of results and rendering reference intervals method dependent. We investigated the degree of equivalence achievable by harmonization and the identification of a commutable secondary reference material to accomplish this goal. We applied technical procedures to achieve harmonization developed by the Consortium for Harmonization of Clinical Laboratory Results. Eleven plasma hepcidin measurement procedures (5 mass spectrometry based and 6 immunochemical based) quantified native individual plasma samples (n = 32) and native plasma pools (n = 8) to assess analytical performance and current and achievable equivalence. In addition, 8 types of candidate reference materials (3 concentrations each, n = 24) were assessed for their suitability, most notably in terms of commutability, to serve as secondary reference material. Absolute hepcidin values and reproducibility (intrameasurement procedure CVs 2.9%-8.7%) differed substantially between measurement procedures, but all were linear and correlated well. The current equivalence (intermeasurement procedure CV 28.6%) between the methods was mainly attributable to differences in calibration and could thus be improved by harmonization with a common calibrator. Linear regression analysis and standardized residuals showed that a candidate reference material consisting of native lyophilized plasma with cryolyoprotectant was commutable for all measurement procedures. Mathematically simulated harmonization with this calibrator resulted in a maximum achievable equivalence of 7.7%. The secondary reference material identified in this study has the potential to substantially improve equivalence between hepcidin measurement procedures and contributes to the establishment of a traceability chain that will ultimately allow standardization of hepcidin measurement results. © 2016 American Association for Clinical Chemistry.

  10. Data Driven Model Development for the Supersonic Semispan Transport (S(sup 4)T)

    NASA Technical Reports Server (NTRS)

    Kukreja, Sunil L.

    2011-01-01

    We investigate two common approaches to model development for robust control synthesis in the aerospace community; namely, reduced order aeroservoelastic modelling based on structural finite-element and computational fluid dynamics based aerodynamic models and a data-driven system identification procedure. It is shown via analysis of experimental Super- Sonic SemiSpan Transport (S4T) wind-tunnel data using a system identification approach it is possible to estimate a model at a fixed Mach, which is parsimonious and robust across varying dynamic pressures.

  11. Communications device identification methods, communications methods, wireless communications readers, wireless communications systems, and articles of manufacture

    DOEpatents

    Steele, Kerry D [Kennewick, WA; Anderson, Gordon A [Benton City, WA; Gilbert, Ronald W [Morgan Hill, CA

    2011-02-01

    Communications device identification methods, communications methods, wireless communications readers, wireless communications systems, and articles of manufacture are described. In one aspect, a communications device identification method includes providing identification information regarding a group of wireless identification devices within a wireless communications range of a reader, using the provided identification information, selecting one of a plurality of different search procedures for identifying unidentified ones of the wireless identification devices within the wireless communications range, and identifying at least some of the unidentified ones of the wireless identification devices using the selected one of the search procedures.

  12. Face-off: A new identification procedure for child eyewitnesses.

    PubMed

    Price, Heather L; Fitzgerald, Ryan J

    2016-09-01

    In 2 experiments, we introduce a new "face-off" procedure for child eyewitness identifications. The new procedure, which is premised on reducing the stimulus set size, was compared with the showup and simultaneous procedures in Experiment 1 and with modified versions of the simultaneous and elimination procedures in Experiment 2. Several benefits of the face-off procedure were observed: it was significantly more diagnostic than the showup procedure; it led to significantly more correct rejections of target-absent lineups than the simultaneous procedures in both experiments, and it led to greater information gain than the modified elimination and simultaneous procedures. The face-off procedure led to consistently more conservative responding than the simultaneous procedures in both experiments. Given the commonly cited concern that children are too lenient in their decision criteria for identification tasks, the face-off procedure may offer a concrete technique to reduce children's high choosing rates. (PsycINFO Database Record (c) 2016 APA, all rights reserved).

  13. 26 CFR 301.7701-12 - Employer identification number.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 26 Internal Revenue 18 2010-04-01 2010-04-01 false Employer identification number. 301.7701-12 Section 301.7701-12 Internal Revenue INTERNAL REVENUE SERVICE, DEPARTMENT OF THE TREASURY (CONTINUED) PROCEDURE AND ADMINISTRATION PROCEDURE AND ADMINISTRATION Definitions § 301.7701-12 Employer identification...

  14. 3D topography measurements on correlation cells—a new approach to forensic ballistics identifications

    NASA Astrophysics Data System (ADS)

    Song, John; Chu, Wei; Tong, Mingsi; Soons, Johannes

    2014-06-01

    Based on three-dimensional (3D) topography measurements on correlation cells, the National Institute of Standards and Technology (NIST) has developed the ‘NIST Ballistics Identification System (NBIS)’ aimed at accurate ballistics identifications and fast ballistics evidence searches. The 3D topographies are divided into arrays of correlation cells to identify ‘valid correlation areas’ and eliminate ‘invalid correlation areas’ from the matching and identification procedure. A ‘congruent matching cells’ (CMC)’ method using three types of identification parameters of the paired correlation cells (cross correlation function maximum CCFmax, spatial registration position in x-y and registration angle θ) is used for high accuracy ballistics identifications. ‘Synchronous processing’ is proposed for correlating multiple cell pairs at the same time to increase the correlation speed. The proposed NBIS can be used for correlations of both geometrical topographies and optical intensity images. All the correlation parameters and algorithms are in the public domain and subject to open tests. An error rate reporting procedure has been developed that can greatly add to the scientific support for the firearm and toolmark identification specialty, and give confidence to the trier of fact in court proceedings. The NBIS is engineered to employ transparent identification parameters and criteria, statistical models and correlation algorithms. In this way, interoperability between different ballistics identification systems can be more easily achieved. This interoperability will make the NBIS suitable for ballistics identifications and evidence searches with large national databases, such as the National Integrated Ballistic Information Network in the United States.

  15. On using the Hilbert transform for blind identification of complex modes: A practical approach

    NASA Astrophysics Data System (ADS)

    Antunes, Jose; Debut, Vincent; Piteau, Pilippe; Delaune, Xavier; Borsoi, Laurent

    2018-01-01

    The modal identification of dynamical systems under operational conditions, when subjected to wide-band unmeasured excitations, is today a viable alternative to more traditional modal identification approaches based on processing sets of measured FRFs or impulse responses. Among current techniques for performing operational modal identification, the so-called blind identification methods are the subject of considerable investigation. In particular, the SOBI (Second-Order Blind Identification) method was found to be quite efficient. SOBI was originally developed for systems with normal modes. To address systems with complex modes, various extension approaches have been proposed, in particular: (a) Using a first-order state-space formulation for the system dynamics; (b) Building complex analytic signals from the measured responses using the Hilbert transform. In this paper we further explore the latter option, which is conceptually interesting while preserving the model order and size. Focus is on applicability of the SOBI technique for extracting the modal responses from analytic signals built from a set of vibratory responses. The novelty of this work is to propose a straightforward computational procedure for obtaining the complex cross-correlation response matrix to be used for the modal identification procedure. After clarifying subtle aspects of the general theoretical framework, we demonstrate that the correlation matrix of the analytic responses can be computed through a Hilbert transform of the real correlation matrix, so that the actual time-domain responses are no longer required for modal identification purposes. The numerical validation of the proposed technique is presented based on time-domain simulations of a conceptual physical multi-modal system, designed to display modes ranging from normal to highly complex, while keeping modal damping low and nearly independent of the modal complexity, and which can prove very interesting in test bench applications. Numerical results for complex modal identifications are presented, and the quality of the identified modal matrix and modal responses, extracted using the complex SOBI technique and implementing the proposed formulation, is assessed.

  16. Resting State EEG-based biometrics for individual identification using convolutional neural networks.

    PubMed

    Lan Ma; Minett, James W; Blu, Thierry; Wang, William S-Y

    2015-08-01

    Biometrics is a growing field, which permits identification of individuals by means of unique physical features. Electroencephalography (EEG)-based biometrics utilizes the small intra-personal differences and large inter-personal differences between individuals' brainwave patterns. In the past, such methods have used features derived from manually-designed procedures for this purpose. Another possibility is to use convolutional neural networks (CNN) to automatically extract an individual's best and most unique neural features and conduct classification, using EEG data derived from both Resting State with Open Eyes (REO) and Resting State with Closed Eyes (REC). Results indicate that this CNN-based joint-optimized EEG-based Biometric System yields a high degree of accuracy of identification (88%) for 10-class classification. Furthermore, rich inter-personal difference can be found using a very low frequency band (0-2Hz). Additionally, results suggest that the temporal portions over which subjects can be individualized is less than 200 ms.

  17. Full-envelope aerodynamic modeling of the Harrier aircraft

    NASA Technical Reports Server (NTRS)

    Mcnally, B. David

    1986-01-01

    A project to identify a full-envelope model of the YAV-8B Harrier using flight-test and parameter identification techniques is described. As part of the research in advanced control and display concepts for V/STOL aircraft, a full-envelope aerodynamic model of the Harrier is identified, using mathematical model structures and parameter identification methods. A global-polynomial model structure is also used as a basis for the identification of the YAV-8B aerodynamic model. State estimation methods are used to ensure flight data consistency prior to parameter identification.Equation-error methods are used to identify model parameters. A fixed-base simulator is used extensively to develop flight test procedures and to validate parameter identification software. Using simple flight maneuvers, a simulated data set was created covering the YAV-8B flight envelope from about 0.3 to 0.7 Mach and about -5 to 15 deg angle of attack. A singular value decomposition implementation of the equation-error approach produced good parameter estimates based on this simulated data set.

  18. Evidence Clearinghouses and Registries: Methods for Locating and Including Studies in Evidence Syntheses

    ERIC Educational Resources Information Center

    Foster, Lisa; LaSota, Robin; Yeide, Martha

    2015-01-01

    The purpose of this investigation is to report about study identification practices across evidence-based registries and clearinghouses in social policy fields, which serve as a resource for scientific, evidence-based decision-making about practices about desired outcomes in these social policy fields. The information retrieval procedures of the…

  19. 9 CFR 147.15 - Laboratory procedure recommended for the bacteriological examination of mycoplasma reactors. 11

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    .... W., Jr., “Mycoplasmosis.” In: Isolation and Identification of Avian Pathogens. (Stephen B. Hitchner... Scientific Company. (4) Mycoplasma Broth Base, dextrose, phenol red, and cysteine hydrochloride are added to... Mycoplasma Broth Base may be obtained from: (a) Product #M 33600, Gibco Diagnostics, 2801 Industrial Drive...

  20. On Biometrics With Eye Movements.

    PubMed

    Zhang, Youming; Juhola, Martti

    2017-09-01

    Eye movements are a relatively novel data source for biometric identification. When video cameras applied to eye tracking become smaller and more efficient, this data source could offer interesting opportunities for the development of eye movement biometrics. In this paper, we study primarily biometric identification as seen as a classification task of multiple classes, and secondarily biometric verification considered as binary classification. Our research is based on the saccadic eye movement signal measurements from 109 young subjects. In order to test the data measured, we use a procedure of biometric identification according to the one-versus-one (subject) principle. In a development from our previous research, which also involved biometric verification based on saccadic eye movements, we now apply another eye movement tracker device with a higher sampling frequency of 250 Hz. The results obtained are good, with correct identification rates at 80-90% at their best.

  1. Adaptive identification of vessel's added moments of inertia with program motion

    NASA Astrophysics Data System (ADS)

    Alyshev, A. S.; Melnikov, V. G.

    2018-05-01

    In this paper, we propose a new experimental method for determining the moments of inertia of the ship model. The paper gives a brief review of existing methods, a description of the proposed method and experimental stand, test procedures and calculation formulas and experimental results. The proposed method is based on the energy approach with special program motions. The ship model is fixed in a special rack consisting of a torsion element and a set of additional servo drives with flywheels (reactive wheels), which correct the motion. The servo drives with an adaptive controller provide the symmetry of the motion, which is necessary for the proposed identification procedure. The effectiveness of the proposed approach is confirmed by experimental results.

  2. Reliability of sensor-based real-time workflow recognition in laparoscopic cholecystectomy.

    PubMed

    Kranzfelder, Michael; Schneider, Armin; Fiolka, Adam; Koller, Sebastian; Reiser, Silvano; Vogel, Thomas; Wilhelm, Dirk; Feussner, Hubertus

    2014-11-01

    Laparoscopic cholecystectomy is a very common minimally invasive surgical procedure that may be improved by autonomous or cooperative assistance support systems. Model-based surgery with a precise definition of distinct procedural tasks (PT) of the operation was implemented and tested to depict and analyze the process of this procedure. Reliability of real-time workflow recognition in laparoscopic cholecystectomy ([Formula: see text] cases) was evaluated by continuous sensor-based data acquisition. Ten PTs were defined including begin/end preparation calots' triangle, clipping/cutting cystic artery and duct, begin/end gallbladder dissection, begin/end hemostasis, gallbladder removal, and end of operation. Data acquisition was achieved with continuous instrument detection, room/table light status, intra-abdominal pressure, table tilt, irrigation/aspiration volume and coagulation/cutting current application. Two independent observers recorded start and endpoint of each step by analysis of the sensor data. The data were cross-checked with laparoscopic video recordings serving as gold standard for PT identification. Bland-Altman analysis revealed for 95% of cases a difference of annotation results within the limits of agreement ranging from [Formula: see text]309 s (PT 7) to +368 s (PT 5). Laparoscopic video and sensor data matched to a greater or lesser extent within the different procedural tasks. In the majority of cases, the observer results exceeded those obtained from the laparoscopic video. Empirical knowledge was required to detect phase transit. A set of sensors used to monitor laparoscopic cholecystectomy procedures was sufficient to enable expert observers to reliably identify each PT. In the future, computer systems may automate the task identification process provided a more robust data inflow is available.

  3. Routine use of PCR-restriction fragment length polymorphism analysis for identification of mycobacteria growing in liquid media.

    PubMed Central

    Taylor, T B; Patterson, C; Hale, Y; Safranek, W W

    1997-01-01

    A PCR-restriction fragment length polymorphism (PCR-RFLP) procedure capable of rapidly identifying 28 species of clinically encountered mycobacteria was evaluated for use in the routine identification of acid-fast isolates growing in BACTEC 12B and 13A liquid media. PCR-RFLP identified 100 of 103 acid-fast isolates recovered from 610 patient specimens submitted for culture during the study. The three isolates unidentifiable by PCR-RFLP produced restriction patterns not included in the PCR-RFLP algorithm and could therefore not be assigned to a species. These isolates were characterized by their morphologic and biochemical characteristics. Two of the isolates were identified as M. terrae complex and M. gordonae. The third isolate could not be definitively identified and could only be characterized as a Mycobacterium sp. most closely resembling M. chelonae. PCR-RFLP identifications agreed with the conventional identifications for 96 of the 100 isolates identified by PCR-RFLP. Subsequent identification of the four discordant isolates by gas chromatography analysis supported the PCR-RFLP identification of each isolate. Amplification products were also obtained from isolates of Streptococcus albus and Rhodococcus equi recovered from patient specimens; however, the restriction patterns of these nonmycobacterial species did not resemble the patterns of any mycobacterial species included in the PCR-RFLP algorithm. PCR-RFLP seems to be a reliable procedure for the routine identification of mycobacteria and has the potential for providing identifications of mycobacterial isolates which are more accurate than conventional identification techniques based on morphologic and biochemical characteristics. PMID:8968884

  4. Data Driven Model Development for the SuperSonic SemiSpan Transport (S(sup 4)T)

    NASA Technical Reports Server (NTRS)

    Kukreja, Sunil L.

    2011-01-01

    In this report, we will investigate two common approaches to model development for robust control synthesis in the aerospace community; namely, reduced order aeroservoelastic modelling based on structural finite-element and computational fluid dynamics based aerodynamic models, and a data-driven system identification procedure. It is shown via analysis of experimental SuperSonic SemiSpan Transport (S4T) wind-tunnel data that by using a system identification approach it is possible to estimate a model at a fixed Mach, which is parsimonious and robust across varying dynamic pressures.

  5. Trial-Based Functional Analysis Informs Treatment for Vocal Scripting.

    PubMed

    Rispoli, Mandy; Brodhead, Matthew; Wolfe, Katie; Gregori, Emily

    2018-05-01

    Research on trial-based functional analysis has primarily focused on socially maintained challenging behaviors. However, procedural modifications may be necessary to clarify ambiguous assessment results. The purposes of this study were to evaluate the utility of iterative modifications to trial-based functional analysis on the identification of putative reinforcement and subsequent treatment for vocal scripting. For all participants, modifications to the trial-based functional analysis identified a primary function of automatic reinforcement. The structure of the trial-based format led to identification of social attention as an abolishing operation for vocal scripting. A noncontingent attention treatment was evaluated using withdrawal designs for each participant. This noncontingent attention treatment resulted in near zero levels of vocal scripting for all participants. Implications for research and practice are presented.

  6. Economic Analyses in Anterior Cruciate Ligament Reconstruction: A Qualitative and Systematic Review.

    PubMed

    Saltzman, Bryan M; Cvetanovich, Gregory L; Nwachukwu, Benedict U; Mall, Nathan A; Bush-Joseph, Charles A; Bach, Bernard R

    2016-05-01

    As the health care system in the United States (US) transitions toward value-based care, there is an increased emphasis on understanding the cost drivers and high-value procedures within orthopaedics. To date, there has been no systematic review of the economic literature on anterior cruciate ligament reconstruction (ACLR). To evaluate the overall evidence base for economic studies published on ACLR in the orthopaedic literature. Data available on the economics of ACLR are summarized and cost drivers associated with the procedure are identified. Systematic review. All economic studies (including US-based and non-US-based) published between inception of the MEDLINE database and October 3, 2014, were identified. Given the heterogeneity of the existing evidence base, a qualitative, descriptive approach was used to assess the collective results from the economic studies on ACLR. When applicable, comparisons were made for the following cost-related variables associated with the procedure for economic implications: outpatient versus inpatient surgery (or outpatient vs overnight hospital stay vs >1-night stay); bone-patellar tendon-bone (BPTB) graft versus hamstring (HS) graft source; autograft versus allograft source; staged unilateral ACLR versus bilateral ACLR in a single setting; single- versus double-bundle technique; ACLR versus nonoperative treatment; and other unique comparisons reported in single studies, including computer-assisted navigation surgery (CANS) versus traditional surgery, early versus delayed ACLR, single- versus double-incision technique, and finally the costs of ACLR without comparison of variables. A total of 24 studies were identified and included; of these, 17 included studies were cost identification studies. The remaining 7 studies were cost utility analyses that used economic models to investigate the effect of variables such as the cost of allograft tissue, fixation devices, and physical therapy, the percentage and timing of revision surgery, and the cost of revision surgery. Of the 24 studies, there were 3 studies with level 1 evidence, 8 with level 2 evidence, 6 with level 3 evidence, and 7 with level 4 evidence. The following economic comparisons were demonstrated: (1) ACLR is more cost-effective than nonoperative treatment with rehabilitation only (per 3 cost utility analyses); (2) autograft use had lower total costs than allograft use, with operating room supply costs and allograft costs most significant (per 5 cost identification studies and 1 cost utility analysis); (3) results on hamstring versus BPTB graft source are conflicting (per 2 cost identification studies); (4) there is significant cost reduction with an outpatient versus inpatient setting (per 5 studies using cost identification analyses); (5) bilateral ACLR is more cost efficient than 2 unilateral ACLRs in separate settings (per 2 cost identification studies); (6) there are lower costs with similarly successful outcomes between single- and double-bundle technique (per 3 cost identification studies and 2 cost utility analyses). Results from this review suggest that early single-bundle, single (endoscopic)-incision outpatient ACLR using either BPTB or HS autograft provides the most value. In the setting of bilateral ACL rupture, single-setting bilateral ACLR is more cost-effective than staged unilateral ACLR. Procedures using CANS technology do not yet yield results that are superior to the results of a standard surgical procedure, and CANS has substantially greater costs. © 2015 The Author(s).

  7. 18 CFR 701.304 - Procedures for identification of individuals making requests.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... 18 Conservation of Power and Water Resources 2 2013-04-01 2012-04-01 true Procedures for identification of individuals making requests. 701.304 Section 701.304 Conservation of Power and Water Resources WATER RESOURCES COUNCIL COUNCIL ORGANIZATION Protection of Privacy § 701.304 Procedures for...

  8. 18 CFR 701.304 - Procedures for identification of individuals making requests.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 18 Conservation of Power and Water Resources 2 2014-04-01 2014-04-01 false Procedures for identification of individuals making requests. 701.304 Section 701.304 Conservation of Power and Water Resources WATER RESOURCES COUNCIL COUNCIL ORGANIZATION Protection of Privacy § 701.304 Procedures for...

  9. 18 CFR 701.304 - Procedures for identification of individuals making requests.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 18 Conservation of Power and Water Resources 2 2010-04-01 2010-04-01 false Procedures for identification of individuals making requests. 701.304 Section 701.304 Conservation of Power and Water Resources WATER RESOURCES COUNCIL COUNCIL ORGANIZATION Protection of Privacy § 701.304 Procedures for...

  10. 18 CFR 701.304 - Procedures for identification of individuals making requests.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 18 Conservation of Power and Water Resources 2 2011-04-01 2011-04-01 false Procedures for identification of individuals making requests. 701.304 Section 701.304 Conservation of Power and Water Resources WATER RESOURCES COUNCIL COUNCIL ORGANIZATION Protection of Privacy § 701.304 Procedures for...

  11. 18 CFR 701.304 - Procedures for identification of individuals making requests.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... 18 Conservation of Power and Water Resources 2 2012-04-01 2012-04-01 false Procedures for identification of individuals making requests. 701.304 Section 701.304 Conservation of Power and Water Resources WATER RESOURCES COUNCIL COUNCIL ORGANIZATION Protection of Privacy § 701.304 Procedures for...

  12. Normalized inverse characterization of sound absorbing rigid porous media.

    PubMed

    Zieliński, Tomasz G

    2015-06-01

    This paper presents a methodology for the inverse characterization of sound absorbing rigid porous media, based on standard measurements of the surface acoustic impedance of a porous sample. The model parameters need to be normalized to have a robust identification procedure which fits the model-predicted impedance curves with the measured ones. Such a normalization provides a substitute set of dimensionless (normalized) parameters unambiguously related to the original model parameters. Moreover, two scaling frequencies are introduced, however, they are not additional parameters and for different, yet reasonable, assumptions of their values, the identification procedure should eventually lead to the same solution. The proposed identification technique uses measured and computed impedance curves for a porous sample not only in the standard configuration, that is, set to the rigid termination piston in an impedance tube, but also with air gaps of known thicknesses between the sample and the piston. Therefore, all necessary analytical formulas for sound propagation in double-layered media are provided. The methodology is illustrated by one numerical test and by two examples based on the experimental measurements of the acoustic impedance and absorption of porous ceramic samples of different thicknesses and a sample of polyurethane foam.

  13. 40 CFR Appendix Xviii to Part 86 - Statistical Outlier Identification Procedure for Light-Duty Vehicles and Light Light-Duty Trucks...

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 40 Protection of Environment 19 2011-07-01 2011-07-01 false Statistical Outlier Identification... (CONTINUED) Pt. 86, App. XVIII Appendix XVIII to Part 86—Statistical Outlier Identification Procedure for..., but suffer theoretical deficiencies if statistical significance tests are required. Consequently, the...

  14. 40 CFR Appendix Xviii to Part 86 - Statistical Outlier Identification Procedure for Light-Duty Vehicles and Light Light-Duty Trucks...

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 40 Protection of Environment 19 2010-07-01 2010-07-01 false Statistical Outlier Identification... (CONTINUED) Pt. 86, App. XVIII Appendix XVIII to Part 86—Statistical Outlier Identification Procedure for..., but suffer theoretical deficiencies if statistical significance tests are required. Consequently, the...

  15. Research in digital adaptive flight controllers

    NASA Technical Reports Server (NTRS)

    Kaufman, H.

    1976-01-01

    A design study of adaptive control logic suitable for implementation in modern airborne digital flight computers was conducted. Both explicit controllers which directly utilize parameter identification and implicit controllers which do not require identification were considered. Extensive analytical and simulation efforts resulted in the recommendation of two explicit digital adaptive flight controllers. Interface weighted least squares estimation procedures with control logic were developed using either optimal regulator theory or with control logic based upon single stage performance indices.

  16. Activities identification for activity-based cost/management applications of the diagnostics outpatient procedures.

    PubMed

    Alrashdan, Abdalla; Momani, Amer; Ababneh, Tamador

    2012-01-01

    One of the most challenging problems facing healthcare providers is to determine the actual cost for their procedures, which is important for internal accounting and price justification to insurers. The objective of this paper is to find suitable categories to identify the diagnostic outpatient medical procedures and translate them from functional orientation to process orientation. A hierarchal task tree is developed based on a classification schema of procedural activities. Each procedure is seen as a process consisting of a number of activities. This makes a powerful foundation for activity-based cost/management implementation and provides enough information to discover the value-added and non-value-added activities that assist in process improvement and eventually may lead to cost reduction. Work measurement techniques are used to identify the standard time of each activity at the lowest level of the task tree. A real case study at a private hospital is presented to demonstrate the proposed methodology. © 2011 National Association for Healthcare Quality.

  17. Identification and quantitation of semi-crystalline microplastics using image analysis and differential scanning calorimetry.

    PubMed

    Rodríguez Chialanza, Mauricio; Sierra, Ignacio; Pérez Parada, Andrés; Fornaro, Laura

    2018-06-01

    There are several techniques used to analyze microplastics. These are often based on a combination of visual and spectroscopic techniques. Here we introduce an alternative workflow for identification and mass quantitation through a combination of optical microscopy with image analysis (IA) and differential scanning calorimetry (DSC). We studied four synthetic polymers with environmental concern: low and high density polyethylene (LDPE and HDPE, respectively), polypropylene (PP), and polyethylene terephthalate (PET). Selected experiments were conducted to investigate (i) particle characterization and counting procedures based on image analysis with open-source software, (ii) chemical identification of microplastics based on DSC signal processing, (iii) dependence of particle size on DSC signal, and (iv) quantitation of microplastics mass based on DSC signal. We describe the potential and limitations of these techniques to increase reliability for microplastic analysis. Particle size demonstrated to have particular incidence in the qualitative and quantitative performance of DSC signals. Both, identification (based on characteristic onset temperature) and mass quantitation (based on heat flow) showed to be affected by particle size. As a result, a proper sample treatment which includes sieving of suspended particles is particularly required for this analytical approach.

  18. Statistical Analyses of Scatterplots to Identify Important Factors in Large-Scale Simulations

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Kleijnen, J.P.C.; Helton, J.C.

    1999-04-01

    The robustness of procedures for identifying patterns in scatterplots generated in Monte Carlo sensitivity analyses is investigated. These procedures are based on attempts to detect increasingly complex patterns in the scatterplots under consideration and involve the identification of (1) linear relationships with correlation coefficients, (2) monotonic relationships with rank correlation coefficients, (3) trends in central tendency as defined by means, medians and the Kruskal-Wallis statistic, (4) trends in variability as defined by variances and interquartile ranges, and (5) deviations from randomness as defined by the chi-square statistic. The following two topics related to the robustness of these procedures are consideredmore » for a sequence of example analyses with a large model for two-phase fluid flow: the presence of Type I and Type II errors, and the stability of results obtained with independent Latin hypercube samples. Observations from analysis include: (1) Type I errors are unavoidable, (2) Type II errors can occur when inappropriate analysis procedures are used, (3) physical explanations should always be sought for why statistical procedures identify variables as being important, and (4) the identification of important variables tends to be stable for independent Latin hypercube samples.« less

  19. Eyewitness identification: Bayesian information gain, base-rate effect equivalency curves, and reasonable suspicion.

    PubMed

    Wells, Gary L; Yang, Yueran; Smalarz, Laura

    2015-04-01

    We provide a novel Bayesian treatment of the eyewitness identification problem as it relates to various system variables, such as instruction effects, lineup presentation format, lineup-filler similarity, lineup administrator influence, and show-ups versus lineups. We describe why eyewitness identification is a natural Bayesian problem and how numerous important observations require careful consideration of base rates. Moreover, we argue that the base rate in eyewitness identification should be construed as a system variable (under the control of the justice system). We then use prior-by-posterior curves and information-gain curves to examine data obtained from a large number of published experiments. Next, we show how information-gain curves are moderated by system variables and by witness confidence and we note how information-gain curves reveal that lineups are consistently more proficient at incriminating the guilty than they are at exonerating the innocent. We then introduce a new type of analysis that we developed called base rate effect-equivalency (BREE) curves. BREE curves display how much change in the base rate is required to match the impact of any given system variable. The results indicate that even relatively modest changes to the base rate can have more impact on the reliability of eyewitness identification evidence than do the traditional system variables that have received so much attention in the literature. We note how this Bayesian analysis of eyewitness identification has implications for the question of whether there ought to be a reasonable-suspicion criterion for placing a person into the jeopardy of an identification procedure. (c) 2015 APA, all rights reserved).

  20. Surgical instrument similarity metrics and tray analysis for multi-sensor instrument identification

    NASA Astrophysics Data System (ADS)

    Glaser, Bernhard; Schellenberg, Tobias; Franke, Stefan; Dänzer, Stefan; Neumuth, Thomas

    2015-03-01

    A robust identification of the instrument currently used by the surgeon is crucial for the automatic modeling and analysis of surgical procedures. Various approaches for intra-operative surgical instrument identification have been presented, mostly based on radio-frequency identification (RFID) or endoscopic video analysis. A novel approach is to identify the instruments on the instrument table of the scrub nurse with a combination of video and weight information. In a previous article, we successfully followed this approach and applied it to multiple instances of an ear, nose and throat (ENT) procedure and the surgical tray used therein. In this article, we present a metric for the suitability of the instruments of a surgical tray for identification by video and weight analysis and apply it to twelve trays of four different surgical domains (abdominal surgery, neurosurgery, orthopedics and urology). The used trays were digitized at the central sterile services department of the hospital. The results illustrate that surgical trays differ in their suitability for the approach. In general, additional weight information can significantly contribute to the successful identification of surgical instruments. Additionally, for ten different surgical instruments, ten exemplars of each instrument were tested for their weight differences. The samples indicate high weight variability in instruments with identical brand and model number. The results present a new metric for approaches aiming towards intra-operative surgical instrument detection and imply consequences for algorithms exploiting video and weight information for identification purposes.

  1. Estimation of radiative and conductive properties of a semitransparent medium using genetic algorithms

    NASA Astrophysics Data System (ADS)

    Braiek, A.; Adili, A.; Albouchi, F.; Karkri, M.; Ben Nasrallah, S.

    2016-06-01

    The aim of this work is to simultaneously identify the conductive and radiative parameters of a semitransparent sample using a photothermal method associated with an inverse problem. The identification of the conductive and radiative proprieties is performed by the minimization of an objective function that represents the errors between calculated temperature and measured signal. The calculated temperature is obtained from a theoretical model built with the thermal quadrupole formalism. Measurement is obtained in the rear face of the sample whose front face is excited by a crenel of heat flux. For identification procedure, a genetic algorithm is developed and used. The genetic algorithm is a useful tool in the simultaneous estimation of correlated or nearly correlated parameters, which can be a limiting factor for the gradient-based methods. The results of the identification procedure show the efficiency and the stability of the genetic algorithm to simultaneously estimate the conductive and radiative properties of clear glass.

  2. Space-Based Identification of Archaeological Illegal Excavations and a New Automatic Method for Looting Feature Extraction in Desert Areas

    NASA Astrophysics Data System (ADS)

    Lasaponara, Rosa; Masini, Nicola

    2018-06-01

    The identification and quantification of disturbance of archaeological sites has been generally approached by visual inspection of optical aerial or satellite pictures. In this paper, we briefly summarize the state of the art of the traditionally satellite-based approaches for looting identification and propose a new automatic method for archaeological looting feature extraction approach (ALFEA). It is based on three steps: the enhancement using spatial autocorrelation, unsupervised classification, and segmentation. ALFEA has been applied to Google Earth images of two test areas, selected in desert environs in Syria (Dura Europos), and in Peru (Cahuachi-Nasca). The reliability of ALFEA was assessed through field surveys in Peru and visual inspection for the Syrian case study. Results from the evaluation procedure showed satisfactory performance from both of the two analysed test cases with a rate of success higher than 90%.

  3. Application of GIS-based Procedure on Slopeland Use Classification and Identification

    NASA Astrophysics Data System (ADS)

    KU, L. C.; LI, M. C.

    2016-12-01

    In Taiwan, the "Slopeland Conservation and Utilization Act" regulates the management of the slopelands. It categorizes the slopeland into land suitable for agricultural or animal husbandry, land suitable for forestry and land for enhanced conservation, according to the environmental factors of average slope, effective soil depth, soil erosion and parental rock. Traditionally, investigations of environmental factors require cost-effective field works. It has been confronted with many practical issues such as non-evaluated cadastral parcels, evaluation results depending on expert's opinion, difficulties in field measurement and judgment, and time consuming. This study aimed to develop a GIS-based procedure involved in the acceleration of slopeland use classification and quality improvement. First, the environmental factors of slopelands were analyzed by GIS and SPSS software. The analysis involved with the digital elevation model (DEM), soil depth map, land use map and satellite images. Second, 5% of the analyzed slopelands were selected to perform the site investigations and correct the results of classification. Finally, a 2nd examination was involved by randomly selected 2% of the analyzed slopelands to perform the accuracy evaluation. It was showed the developed procedure is effective in slopeland use classification and identification. Keywords: Slopeland Use Classification, GIS, Management

  4. Rapid microbiochemical identification of Corynebacterium diphtheriae and other medically important corynebacteria.

    PubMed Central

    Thompson, J S; Gates-Davis, D R; Yong, D C

    1983-01-01

    A rapid biochemical method based on the fermentation of carbohydrates, the hydrolysis of urea, and the reduction of nitrate was used to identify Corynebacterium diphtheriae, C. ulcerans, C. pseudodiphtheriticum, C. haemolyticum, C. pseudotuberculosis, C. pyogenes, C. ovis, the Centers for Disease Control JK group, and Rhodococcus (Corynebacterium) equi. With this procedure identification was confirmed for 133 stock cultures and clinical isolates of corynebacteria. Most were identified within 1 h and all were identified within 4 h after inoculation into the test substrates. PMID:6355166

  5. Development of a toxicity identification evaluation procedure for characterizing metal toxicity in marine sediments

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Burgess, R.M.; Cantwell, M.G.; Pelletier, M.C.

    2000-04-01

    A multiagency effort is underway to develop whole sediment toxicity identification evaluation (TIE) methods. Whole sediment TIE methods will be critical tools for characterizing toxicity at hazardous waste sites and in the conduct of environmental risk assessments. The research approach is based on the predominance of three classes of toxicants in sediments: ammonia, nonpolar organic chemicals, and metals. Here the authors describe a procedure for characterizing acute toxicity caused by metals in whole marine sediments. The procedure involves adding a chelating resin to sediments, resulting in the sequestration of bioavailable metal while not stressing testing organisms. Within the testing chambers,more » the presence of resin resulted in statistically significant reductions in the overlying and interstitial water concentrations of five metals (cadmium, copper, nickel, lead, and zinc) generally by factors of 40 and 200. Toxicity to both the amphipod Ampelisca abdita and mysid Americamysis bahia (formerly Mysidopsis bahia) of sediments spiked with the five metals was decreased by approximately a factor of four when resin was present. While very effective at reducing the concentrations and toxicity of metals, the resin has only minor ameliorative effects on the toxicity of ammonia and a representative nonpolar toxicant (Endosulfan). Resin and accumulated metal were easily isolated from the testing system following exposures allowing for the initiation of phase II TIE (identification) procedures. This procedure using the addition of a chelating resin provides an approach for determining the importance of metals to the toxicity of marine sediments. Work is continuing to validate the method with environmentally contaminated sediments.« less

  6. An algorithm to diagnose ball bearing faults in servomotors running arbitrary motion profiles

    NASA Astrophysics Data System (ADS)

    Cocconcelli, Marco; Bassi, Luca; Secchi, Cristian; Fantuzzi, Cesare; Rubini, Riccardo

    2012-02-01

    This paper describes a procedure to extend the scope of classical methods to detect ball bearing faults (based on envelope analysis and fault frequencies identification) beyond their usual area of application. The objective of this procedure is to allow condition-based monitoring of such bearings in servomotor applications, where typically the motor in its normal mode of operation has to follow a non-constant angular velocity profile that may contain motion inversions. After describing and analyzing the algorithm from a theoretical point of view, experimental results obtained on a real industrial application are presented and commented.

  7. 28 CFR 513.11 - Procedures for requesting a FBI identification record or a NCIC/III record.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 28 Judicial Administration 2 2011-07-01 2011-07-01 false Procedures for requesting a FBI..., DEPARTMENT OF JUSTICE GENERAL MANAGEMENT AND ADMINISTRATION ACCESS TO RECORDS Production or Disclosure of FBI/NCIC Information § 513.11 Procedures for requesting a FBI identification record or a NCIC/III record...

  8. 28 CFR 513.11 - Procedures for requesting a FBI identification record or a NCIC/III record.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 28 Judicial Administration 2 2012-07-01 2012-07-01 false Procedures for requesting a FBI..., DEPARTMENT OF JUSTICE GENERAL MANAGEMENT AND ADMINISTRATION ACCESS TO RECORDS Production or Disclosure of FBI/NCIC Information § 513.11 Procedures for requesting a FBI identification record or a NCIC/III record...

  9. 28 CFR 513.11 - Procedures for requesting a FBI identification record or a NCIC/III record.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 28 Judicial Administration 2 2013-07-01 2013-07-01 false Procedures for requesting a FBI..., DEPARTMENT OF JUSTICE GENERAL MANAGEMENT AND ADMINISTRATION ACCESS TO RECORDS Production or Disclosure of FBI/NCIC Information § 513.11 Procedures for requesting a FBI identification record or a NCIC/III record...

  10. 28 CFR 513.11 - Procedures for requesting a FBI identification record or a NCIC/III record.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 28 Judicial Administration 2 2014-07-01 2014-07-01 false Procedures for requesting a FBI..., DEPARTMENT OF JUSTICE GENERAL MANAGEMENT AND ADMINISTRATION ACCESS TO RECORDS Production or Disclosure of FBI/NCIC Information § 513.11 Procedures for requesting a FBI identification record or a NCIC/III record...

  11. 28 CFR 513.11 - Procedures for requesting a FBI identification record or a NCIC/III record.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 28 Judicial Administration 2 2010-07-01 2010-07-01 false Procedures for requesting a FBI..., DEPARTMENT OF JUSTICE GENERAL MANAGEMENT AND ADMINISTRATION ACCESS TO RECORDS Production or Disclosure of FBI/NCIC Information § 513.11 Procedures for requesting a FBI identification record or a NCIC/III record...

  12. Time domain nonlinear SMA damper force identification approach and its numerical validation

    NASA Astrophysics Data System (ADS)

    Xin, Lulu; Xu, Bin; He, Jia

    2012-04-01

    Most of the currently available vibration-based identification approaches for structural damage detection are based on eigenvalues and/or eigenvectors extracted from vibration measurements and, strictly speaking, are only suitable for linear system. However, the initiation and development of damage in engineering structures under severe dynamic loadings are typical nonlinear procedure. Studies on the identification of restoring force which is a direct indicator of the extent of the nonlinearity have received increasing attention in recent years. In this study, a date-based time domain identification approach for general nonlinear system was developed. The applied excitation and the corresponding response time series of the structure were used for identification by means of standard least-square techniques and a power series polynomial model (PSPM) which was utilized to model the nonlinear restoring force (NRF). The feasibility and robustness of the proposed approach was verified by a 2 degree-of-freedoms (DOFs) lumped mass numerical model equipped with a shape memory ally (SMA) damper mimicking nonlinear behavior. The results show that the proposed data-based time domain method is capable of identifying the NRF in engineering structures without any assumptions on the mass distribution and the topology of the structure, and provides a promising way for damage detection in the presence of structural nonlinearities.

  13. Thioredoxin targets in plants: The first 30 years

    USDA-ARS?s Scientific Manuscript database

    The turn of the century welcomed major developments in redox biology. In one development with plants, proteomics made possible the identification of proteins linked to thioredoxin (Trx), initially in chloroplasts and then other cell compartments. Two procedures, one based on thiol specific probes an...

  14. A Bayesian Approach for Sensor Optimisation in Impact Identification

    PubMed Central

    Mallardo, Vincenzo; Sharif Khodaei, Zahra; Aliabadi, Ferri M. H.

    2016-01-01

    This paper presents a Bayesian approach for optimizing the position of sensors aimed at impact identification in composite structures under operational conditions. The uncertainty in the sensor data has been represented by statistical distributions of the recorded signals. An optimisation strategy based on the genetic algorithm is proposed to find the best sensor combination aimed at locating impacts on composite structures. A Bayesian-based objective function is adopted in the optimisation procedure as an indicator of the performance of meta-models developed for different sensor combinations to locate various impact events. To represent a real structure under operational load and to increase the reliability of the Structural Health Monitoring (SHM) system, the probability of malfunctioning sensors is included in the optimisation. The reliability and the robustness of the procedure is tested with experimental and numerical examples. Finally, the proposed optimisation algorithm is applied to a composite stiffened panel for both the uniform and non-uniform probability of impact occurrence. PMID:28774064

  15. Identification of quasi-steady compressor characteristics from transient data

    NASA Technical Reports Server (NTRS)

    Nunes, K. B.; Rock, S. M.

    1984-01-01

    The principal goal was to demonstrate that nonlinear compressor map parameters, which govern an in-stall response, can be identified from test data using parameter identification techniques. The tasks included developing and then applying an identification procedure to data generated by NASA LeRC on a hybrid computer. Two levels of model detail were employed. First was a lumped compressor rig model; second was a simplified turbofan model. The main outputs are the tools and procedures generated to accomplish the identification.

  16. Audit of transfusion procedures in 660 hospitals. A College of American Pathologists Q-Probes study of patient identification and vital sign monitoring frequencies in 16494 transfusions.

    PubMed

    Novis, David A; Miller, Karen A; Howanitz, Peter J; Renner, Stephen W; Walsh, Molly K

    2003-05-01

    Hemolytic transfusion reactions are often the result of failure to follow established identification and monitoring procedures. To measure the frequencies with which health care workers completed specific transfusion procedures required for laboratory and blood bank accreditation. In 2 separate studies, participants in the College of American Pathologists Q-Probes laboratory quality improvement program audited nonemergent red blood cell transfusions prospectively and completed questionnaires profiling their institutions' transfusion policies. A total of 660 institutions, predominantly in the United States, at which transfusion medicine services are provided. The percentages of transfusions for which participants completed 4 specific components of patient and blood unit identifications, and for which participants monitored vital signs at 3 specific intervals during transfusions. In the first study, all components of patient identification procedures were performed in 62.3%, and all required patient vital sign monitoring was performed in 81.6% of 12 448 transfusions audited. The median frequencies with which institutions participating in the first study performed all patient identification and monitoring procedures were 69.0% and 90.2%, respectively. In the second study, all components of patient identification were performed in 25.4% and all patient vital sign monitoring was performed in 88.3% of 4046 transfusions audited. The median frequencies with which institutions participating in the second study performed all patient identification and monitoring procedures were 10.0% and 95.0%, respectively. Individual practices and/or institutional policies associated with greater frequencies of patient identification and/or vital sign monitoring included transporting units of blood directly to patient bedsides, having no more than 1 individual handle blood units in route, checking unit labels against physicians' orders, having patients wear identification tags (wristbands), reading identification information aloud when 2 or more transfusionists participated, using written checklists to guide the administration of blood, instructing health care personnel in transfusion practices, and routinely auditing the administration of transfusions. In many hospitals, the functions of identification and vital sign monitoring of patients receiving blood transfusions do not meet laboratory and blood bank accreditation standards. Differences in hospital transfusion policies influence how well health care workers comply with standard practices. We would expect that efforts designed to perfect transfusion policies might also improve performance in those hospitals in which practice compliance is substandard.

  17. Systematic searching for theory to inform systematic reviews: is it feasible? Is it desirable?

    PubMed

    Booth, Andrew; Carroll, Christopher

    2015-09-01

    In recognising the potential value of theory in understanding how interventions work comes a challenge - how to make identification of theory less haphazard? To explore the feasibility of systematic identification of theory. We searched PubMed for published reviews (1998-2012) that had explicitly sought to identify theory. Systematic searching may be characterised by a structured question, methodological filters and an itemised search procedure. We constructed a template (BeHEMoTh - Behaviour of interest; Health context; Exclusions; Models or Theories) for use when systematically identifying theory. The authors tested the template within two systematic reviews. Of 34 systematic reviews, only 12 reviews (35%) reported a method for identifying theory. Nineteen did not specify how they identified studies containing theory. Data were unavailable for three reviews. Candidate terms include concept(s)/conceptual, framework(s), model(s), and theory/theories/theoretical. Information professionals must overcome inadequate reporting and the use of theory out of context. The review team faces an additional concern in lack of 'theory fidelity'. Based on experience with two systematic reviews, the BeHEMoTh template and procedure offers a feasible and useful approach for identification of theory. Applications include realist synthesis, framework synthesis or review of complex interventions. The procedure requires rigorous evaluation. © 2015 Health Libraries Group.

  18. What Happened to the Streptococci: Overview of Taxonomic and Nomenclature Changes

    PubMed Central

    Facklam, Richard

    2002-01-01

    Since the division of the Streptococcus genus into enterococci, lactococci, and streptococci in 1984, many changes in the nomenclature and taxonomy of the Streptococcus genus have taken place. The application of genetic comparisons has improved the proper classification of the different species. The Lancefield system of serogrouping the streptococci by the expression of beta-hemolysis on blood agar plates is still very useful for the identification of streptococci for patient management. The Lancefield grouping system cannot be used in itself for accurate identification of specific beta-hemolytic species, but it can be a useful part of the identification procedure. Except for identification of the “Streptococcus bovis group” of species and Streptococcus suis, Lancefield grouping is of little value in identification of the non-beta-hemolytic streptococci and related genera. In fact, identification of the non-beta-hemolytic species is problematic for conventional as well as commercially available identification procedures. A combination of conventional tests and specific chromogenic tests suggested by several investigators is presented and discussed. Tables are included that suggest tests and procedures to guide investigators attempting to identify all the species. PMID:12364372

  19. Raman spectroscopic studies on bacteria

    NASA Astrophysics Data System (ADS)

    Maquelin, Kees; Choo-Smith, Lin-P'ing; Endtz, Hubert P.; Bruining, Hajo A.; Puppels, Gerwin J.

    2000-11-01

    Routine clinical microbiological identification of pathogenic micro-organisms is largely based on nutritional and biochemical tests. Laboratory results can be presented to a clinician after 2 - 3 days for most clinically relevant micro- organisms. Most of this time is required to obtain pure cultures and enough biomass for the tests to be performed. In the case of severely ill patients, this unavoidable time delay associated with such identification procedures can be fatal. A novel identification method based on confocal Raman microspectroscopy will be presented. With this method it is possible to obtain Raman spectra directly from microbial microcolonies on the solid culture medium, which have developed after only 6 hours of culturing for most commonly encountered organisms. Not only does this technique enable rapid (same day) identifications, but also preserves the sample allowing it to be double-checked with traditional tests. This, combined with the speed and minimal sample handling indicate that confocal Raman microspectroscopy has much potential as a powerful new tool in clinical diagnostic microbiology.

  20. Registering Names and Addresses for Information Technology.

    ERIC Educational Resources Information Center

    Knapp, Arthur A.

    The identification of administrative authorities and the development of associated procedures for registering and accessing names and addresses of communications data systems are considered in this paper. It is noted that, for data communications systems using standards based on the Open Systems Interconnection (OSI) Reference Model specified by…

  1. Material identification of real impact sounds: effects of size variation in steel, glass, wood, and plexiglass plates.

    PubMed

    Giordano, Bruno L; McAdams, Stephen

    2006-02-01

    Identification of the material of struck objects of variable size was investigated. Previous studies on this issue assumed recognition to be based on acoustical measures of damping. This assumption was tested, comparing the power of a damping measure in explaining identification data with that of several other acoustical descriptors. Listeners' performance was perfect with respect to gross material categories (steel-glass and wood-plexiglass) comprising materials of vastly different mechanical properties. Impaired performance was observed for materials within the same gross category, identification being based on the size of the objects alone. The damping descriptor accounted for the identification of the gross categories. However other descriptors such as signal duration explained the results equally well. Materials within the same gross category were identified mainly on the basis of signal frequency. Overall poor support for the relevance of damping to material perception was found. An analysis of the acoustical support for perfect material identification was carried out. Sufficient acoustical information for perfect performance was found. Thus, procedural biases for the origin of the effects of size could be discarded, pointing toward their cognitive, rather than methodological nature. Identification performance was explained in terms of the regularities of the everyday acoustical environment.

  2. Alternatives to the sequential lineup: the importance of controlling the pictures.

    PubMed

    Lindsay, R C; Bellinger, K

    1999-06-01

    Because sequential lineups reduce false-positive choices, their use has been recommended (R. C. L. Lindsay, 1999; R. C. L. Lindsay & G. L. Wells, 1985). Blind testing is included in the recommended procedures. Police, concerned about blind testing, devised alternative procedures, including self-administered sequential lineups, to reduce use of relative judgments (G. L. Wells, 1984) while permitting the investigating officer to conduct the procedure. Identification data from undergraduates exposed to a staged crime (N = 165) demonstrated that 4 alternative identification procedures tested were less effective than the original sequential lineup. Allowing witnesses to control the photographs resulted in higher rates of false-positive identification. Self-reports of using relative judgments were shown to be postdictive of decision accuracy.

  3. Ground Software Maintenance Facility (GSMF) user's manual. Appendices NASA-CR-178806 NAS 1.26:178806 Rept-41849-G159-026-App HC A05/MF A01

    NASA Technical Reports Server (NTRS)

    Aquila, V.; Derrig, D.; Griffith, G.

    1986-01-01

    Procedures are presented that allow the user to assemble tasks, link, compile, backup the system, generate/establish/print display pages, cancel tasks in memory, and to TET an assembly task without having to enter the commands every time. A list of acronyms is provided. Software identification, payload checkout unit operating system services, data base generation, and MITRA operating procedures are also discussed.

  4. Effects of organizational safety on employees' proactivity safety behaviors and occupational health and safety management systems in Chinese high-risk small-scale enterprises.

    PubMed

    Mei, Qiang; Wang, Qiwei; Liu, Suxia; Zhou, Qiaomei; Zhang, Jingjing

    2018-06-07

    Based on the characteristics of small-scale enterprises, the improvement of occupational health and safety management systems (OHS MS) needs an effective intervention. This study proposed a structural equation model and examined the relationships of perceived organization support for safety (POSS), person-organization safety fit (POSF) and proactivity safety behaviors with safety management, safety procedures and safety hazards identification. Data were collected from 503 employees of 105 Chinese high-risk small-scale enterprises over 6 months. The results showed that both POSS and POSF were positively related to improvement in safety management, safety procedures and safety hazards identification through proactivity safety behaviors. Our findings provide a new perspective on organizational safety for improving OHS MS for small-scale enterprises and extend the application of proactivity safety behaviors.

  5. 47 CFR 87.107 - Station identification.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... aircraft followed by the characters of the registration marking (“N” number) of the aircraft, omitting the... type of aircraft followed by the last three characters of the registration marking. Notwithstanding any... Operating Requirements and Procedures Operating Procedures § 87.107 Station identification. (a) Aircraft...

  6. 47 CFR 87.107 - Station identification.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... aircraft followed by the characters of the registration marking (“N” number) of the aircraft, omitting the... type of aircraft followed by the last three characters of the registration marking. Notwithstanding any... Operating Requirements and Procedures Operating Procedures § 87.107 Station identification. (a) Aircraft...

  7. 47 CFR 87.107 - Station identification.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... aircraft followed by the characters of the registration marking (“N” number) of the aircraft, omitting the... type of aircraft followed by the last three characters of the registration marking. Notwithstanding any... Operating Requirements and Procedures Operating Procedures § 87.107 Station identification. (a) Aircraft...

  8. 47 CFR 87.107 - Station identification.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... aircraft followed by the characters of the registration marking (“N” number) of the aircraft, omitting the... type of aircraft followed by the last three characters of the registration marking. Notwithstanding any... Operating Requirements and Procedures Operating Procedures § 87.107 Station identification. (a) Aircraft...

  9. 47 CFR 87.107 - Station identification.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... aircraft followed by the characters of the registration marking (“N” number) of the aircraft, omitting the... type of aircraft followed by the last three characters of the registration marking. Notwithstanding any... Operating Requirements and Procedures Operating Procedures § 87.107 Station identification. (a) Aircraft...

  10. Modified extraction procedure for gas-liquid chromatography applied to the identification of anaerobic bacteria.

    PubMed Central

    Thomann, W R; Hill, G B

    1986-01-01

    Chloroform and ether commonly are used as solvents to extract metabolic organic acids for analysis by gas-liquid chromatography in the identification of anaerobic bacteria. Because these solvents are potentially hazardous to personnel, modified extraction procedures involving the use of a safer solvent, methyl tert-butyl ether were developed which remained both simple to perform and effective for organism identification. PMID:3700623

  11. Automated riverine landscape characterization: GIS-based tools for watershed-scale research, assessment, and management.

    PubMed

    Williams, Bradley S; D'Amico, Ellen; Kastens, Jude H; Thorp, James H; Flotemersch, Joseph E; Thoms, Martin C

    2013-09-01

    River systems consist of hydrogeomorphic patches (HPs) that emerge at multiple spatiotemporal scales. Functional process zones (FPZs) are HPs that exist at the river valley scale and are important strata for framing whole-watershed research questions and management plans. Hierarchical classification procedures aid in HP identification by grouping sections of river based on their hydrogeomorphic character; however, collecting data required for such procedures with field-based methods is often impractical. We developed a set of GIS-based tools that facilitate rapid, low cost riverine landscape characterization and FPZ classification. Our tools, termed RESonate, consist of a custom toolbox designed for ESRI ArcGIS®. RESonate automatically extracts 13 hydrogeomorphic variables from readily available geospatial datasets and datasets derived from modeling procedures. An advanced 2D flood model, FLDPLN, designed for MATLAB® is used to determine valley morphology by systematically flooding river networks. When used in conjunction with other modeling procedures, RESonate and FLDPLN can assess the character of large river networks quickly and at very low costs. Here we describe tool and model functions in addition to their benefits, limitations, and applications.

  12. Improved Estimates of Clear Sky Longwave Flux and Application to the Tropical Greenhouse Effect

    NASA Technical Reports Server (NTRS)

    Collins, W. D.

    1997-01-01

    The first objective of this investigation is to eliminate the clear-sky offset introduced by the scene-identification procedures developed for the Earth Radiation Budget Experiment (ERBE). Estimates of this systematic bias range from 10 to as high as 30 W/sq m. The initial version of the ScaRaB data is being processed with the original ERBE algorithm. Since the ERBE procedure for scene identification is based upon zonal flux averages, clear scenes with longwave emission well below the zonal mean value are mistakenly classified as cloudy. The erroneous classification is more frequent in regions with deep convection and enhanced mid- and upper-tropospheric humidity. We will develop scene identification parameters with zonal and/or time dependence to reduce or eliminate the bias in the clear- sky data. The modified scene identification procedure could be used for the ScaRaB-specific version of the Earth-radiation products. The second objective is to investigate changes in the clear-sky Outgoing Longwave Radiation (OLR) associated with decadal variations in the tropical and subtropical climate. There is considerable evidence for a shift in the climate state starting in approximately 1977. The shift is accompanied by higher SSTs in the equatorial Pacific, increased tropical convection, and higher values of atmospheric humidity. Other evidence indicates that the humidity in the tropical troposphere has been steadily increasing over the last 30 years. It is not known whether the atmospheric greenhouse effect has increased during this period in response to these changes in SST and precipitable water. We will investigate the decadal-scale fluctuations in the greenhouse effect using Nimbus-7, ERBE, and ScaRaB measurements spaning 1979 to the present. The data from the different satellites will be intercalibrated by comparison with model calculations based upon ship radiosonde observations. The fluxes calculated from the radiation model will also be used for validation of the ScaRaB fluxes.

  13. Note: Model identification and analysis of bivalent analyte surface plasmon resonance data.

    PubMed

    Tiwari, Purushottam Babu; Üren, Aykut; He, Jin; Darici, Yesim; Wang, Xuewen

    2015-10-01

    Surface plasmon resonance (SPR) is a widely used, affinity based, label-free biophysical technique to investigate biomolecular interactions. The extraction of rate constants requires accurate identification of the particular binding model. The bivalent analyte model involves coupled non-linear differential equations. No clear procedure to identify the bivalent analyte mechanism has been established. In this report, we propose a unique signature for the bivalent analyte model. This signature can be used to distinguish the bivalent analyte model from other biphasic models. The proposed method is demonstrated using experimentally measured SPR sensorgrams.

  14. A vibration-based health monitoring program for a large and seismically vulnerable masonry dome

    NASA Astrophysics Data System (ADS)

    Pecorelli, M. L.; Ceravolo, R.; De Lucia, G.; Epicoco, R.

    2017-05-01

    Vibration-based health monitoring of monumental structures must rely on efficient and, as far as possible, automatic modal analysis procedures. Relatively low excitation energy provided by traffic, wind and other sources is usually sufficient to detect structural changes, as those produced by earthquakes and extreme events. Above all, in-operation modal analysis is a non-invasive diagnostic technique that can support optimal strategies for the preservation of architectural heritage, especially if complemented by model-driven procedures. In this paper, the preliminary steps towards a fully automated vibration-based monitoring of the world’s largest masonry oval dome (internal axes of 37.23 by 24.89 m) are presented. More specifically, the paper reports on signal treatment operations conducted to set up the permanent dynamic monitoring system of the dome and to realise a robust automatic identification procedure. Preliminary considerations on the effects of temperature on dynamic parameters are finally reported.

  15. Mass spectrometry compatible surfactant for optimized in-gel protein digestion.

    PubMed

    Saveliev, Sergei V; Woodroofe, Carolyn C; Sabat, Grzegorz; Adams, Christopher M; Klaubert, Dieter; Wood, Keith; Urh, Marjeta

    2013-01-15

    Identification of proteins resolved by SDS-PAGE depends on robust in-gel protein digestion and efficient peptide extraction, requirements that are often difficult to achieve. A lengthy and laborious procedure is an additional challenge of protein identification in gel. We show here that with the use of the mass spectrometry compatible surfactant sodium 3-((1-(furan-2-yl)undecyloxy)carbonylamino)propane-1-sulfonate, the challenges of in-gel protein digestion are effectively addressed. Peptide quantitation based on stable isotope labeling showed that the surfactant induced 1.5-2 fold increase in peptide recovery. Consequently, protein sequence coverage was increased by 20-30%, on average, and the number of identified proteins saw a substantial boost. The surfactant also accelerated the digestion process. Maximal in-gel digestion was achieved in as little as one hour, depending on incubation temperature, and peptides were readily recovered from gel eliminating the need for postdigestion extraction. This study shows that the surfactant provides an efficient means of improving protein identification in gel and streamlining the in-gel digestion procedure requiring no extra handling steps or special equipment.

  16. Systematic internal transcribed spacer sequence analysis for identification of clinical mold isolates in diagnostic mycology: a 5-year study.

    PubMed

    Ciardo, Diana E; Lucke, Katja; Imhof, Alex; Bloemberg, Guido V; Böttger, Erik C

    2010-08-01

    The implementation of internal transcribed spacer (ITS) sequencing for routine identification of molds in the diagnostic mycology laboratory was analyzed in a 5-year study. All mold isolates (n = 6,900) recovered in our laboratory from 2005 to 2009 were included in this study. According to a defined work flow, which in addition to troublesome phenotypic identification takes clinical relevance into account, 233 isolates were subjected to ITS sequence analysis. Sequencing resulted in successful identification for 78.6% of the analyzed isolates (57.1% at species level, 21.5% at genus level). In comparison, extended in-depth phenotypic characterization of the isolates subjected to sequencing achieved taxonomic assignment for 47.6% of these, with a mere 13.3% at species level. Optimization of DNA extraction further improved the efficacy of molecular identification. This study is the first of its kind to testify to the systematic implementation of sequence-based identification procedures in the routine workup of mold isolates in the diagnostic mycology laboratory.

  17. Inquiry-Based Approach to a Carbohydrate Analysis Experiment

    NASA Astrophysics Data System (ADS)

    Senkbeil, Edward G.

    1999-01-01

    The analysis of an unknown carbohydrate in an inquiry-based learning format has proven to be a valuable and interesting undergraduate biochemistry laboratory experiment. Students are given a list of carbohydrates and a list of references for carbohydrate analysis. The references contain a variety of well-characterized wet chemistry and instrumental techniques for carbohydrate identification, but the students must develop an appropriate sequential protocol for unknown identification. The students are required to provide a list of chemicals and procedures and a flow chart for identification before the lab. During the 3-hour laboratory period, they utilize their accumulated information and knowledge to classify and identify their unknown. Advantages of the inquiry-based format are (i) students must be well prepared in advance to be successful in the laboratory, (ii) students feel a sense of accomplishment in both designing and carrying out a successful experiment, and (iii) the carbohydrate background information digested by the students significantly decreases the amount of lecture time required for this topic.

  18. Identifying the bad guy in a lineup using confidence judgments under deadline pressure.

    PubMed

    Brewer, Neil; Weber, Nathan; Wootton, David; Lindsay, D Stephen

    2012-10-01

    Eyewitness-identification tests often culminate in witnesses not picking the culprit or identifying innocent suspects. We tested a radical alternative to the traditional lineup procedure used in such tests. Rather than making a positive identification, witnesses made confidence judgments under a short deadline about whether each lineup member was the culprit. We compared this deadline procedure with the traditional sequential-lineup procedure in three experiments with retention intervals ranging from 5 min to 1 week. A classification algorithm that identified confidence criteria that optimally discriminated accurate from inaccurate decisions revealed that decision accuracy was 24% to 66% higher under the deadline procedure than under the traditional procedure. Confidence profiles across lineup stimuli were more informative than were identification decisions about the likelihood that an individual witness recognized the culprit or correctly recognized that the culprit was not present. Large differences between the maximum and the next-highest confidence value signaled very high accuracy. Future support for this procedure across varied conditions would highlight a viable alternative to the problematic lineup procedures that have traditionally been used by law enforcement.

  19. Application of statistical process control and process capability analysis procedures in orbiter processing activities at the Kennedy Space Center

    NASA Technical Reports Server (NTRS)

    Safford, Robert R.; Jackson, Andrew E.; Swart, William W.; Barth, Timothy S.

    1994-01-01

    Successful ground processing at KSC requires that flight hardware and ground support equipment conform to specifications at tens of thousands of checkpoints. Knowledge of conformance is an essential requirement for launch. That knowledge of conformance at every requisite point does not, however, enable identification of past problems with equipment, or potential problem areas. This paper describes how the introduction of Statistical Process Control and Process Capability Analysis identification procedures into existing shuttle processing procedures can enable identification of potential problem areas and candidates for improvements to increase processing performance measures. Results of a case study describing application of the analysis procedures to Thermal Protection System processing are used to illustrate the benefits of the approaches described in the paper.

  20. [Benchmarking in patient identification: An opportunity to learn].

    PubMed

    Salazar-de-la-Guerra, R M; Santotomás-Pajarrón, A; González-Prieto, V; Menéndez-Fraga, M D; Rocha Hurtado, C

    To perform a benchmarking on the safe identification of hospital patients involved in "Club de las tres C" (Calidez, Calidad y Cuidados) in order to prepare a common procedure for this process. A descriptive study was conducted on the patient identification process in palliative care and stroke units in 5medium-stay hospitals. The following steps were carried out: Data collection from each hospital; organisation and data analysis, and preparation of a common procedure for this process. The data obtained for the safe identification of all stroke patients were: hospital 1 (93%), hospital 2 (93.1%), hospital 3 (100%), and hospital 5 (93.4%), and for the palliative care process: hospital 1 (93%), hospital 2 (92.3%), hospital 3 (92%), hospital 4 (98.3%), and hospital 5 (85.2%). The aim of the study has been accomplished successfully. Benchmarking activities have been developed and knowledge on the patient identification process has been shared. All hospitals had good results. The hospital 3 was best in the ictus identification process. The benchmarking identification is difficult, but, a useful common procedure that collects the best practices has been identified among the 5 hospitals. Copyright © 2017 SECA. Publicado por Elsevier España, S.L.U. All rights reserved.

  1. Ontology-based specification, identification and analysis of perioperative risks.

    PubMed

    Uciteli, Alexandr; Neumann, Juliane; Tahar, Kais; Saleh, Kutaiba; Stucke, Stephan; Faulbrück-Röhr, Sebastian; Kaeding, André; Specht, Martin; Schmidt, Tobias; Neumuth, Thomas; Besting, Andreas; Stegemann, Dominik; Portheine, Frank; Herre, Heinrich

    2017-09-06

    Medical personnel in hospitals often works under great physical and mental strain. In medical decision-making, errors can never be completely ruled out. Several studies have shown that between 50 and 60% of adverse events could have been avoided through better organization, more attention or more effective security procedures. Critical situations especially arise during interdisciplinary collaboration and the use of complex medical technology, for example during surgical interventions and in perioperative settings (the period of time before, during and after surgical intervention). In this paper, we present an ontology and an ontology-based software system, which can identify risks across medical processes and supports the avoidance of errors in particular in the perioperative setting. We developed a practicable definition of the risk notion, which is easily understandable by the medical staff and is usable for the software tools. Based on this definition, we developed a Risk Identification Ontology (RIO) and used it for the specification and the identification of perioperative risks. An agent system was developed, which gathers risk-relevant data during the whole perioperative treatment process from various sources and provides it for risk identification and analysis in a centralized fashion. The results of such an analysis are provided to the medical personnel in form of context-sensitive hints and alerts. For the identification of the ontologically specified risks, we developed an ontology-based software module, called Ontology-based Risk Detector (OntoRiDe). About 20 risks relating to cochlear implantation (CI) have already been implemented. Comprehensive testing has indicated the correctness of the data acquisition, risk identification and analysis components, as well as the web-based visualization of results.

  2. Procedural justice, supervisor autonomy support, work satisfaction, organizational identification and job performance: the mediating role of need satisfaction and perceived organizational support.

    PubMed

    Gillet, Nicolas; Colombat, Philippe; Michinov, Estelle; Pronost, Anne-Marie; Fouquereau, Evelyne

    2013-11-01

    To test a model linking procedural justice, supervisor autonomy support, need satisfaction, organizational support, work satisfaction, organizational identification and job performance. Research in industrial and organizational psychology has shown that procedural justice and supervisor autonomy support lead to positive outcomes. However, very little research related to this subject has been conducted in healthcare settings. Moreover, few studies have examined mechanisms that could account for these positive relationships. A cross-sectional correlational design was used. Convenience sampling was used and a sample of 500 nurses working in haematology, oncology and haematology/oncology units in France was surveyed in 2011. The final sample consisted of 323 nurses (64.6% response rate). The hypothesized model was tested using structural equation modelling. Procedural justice and supervisor autonomy support significantly and positively influenced need satisfaction and perceived organizational support, which in turn positively predicted work satisfaction, organizational identification and job performance. Organizations could deliver training programmes for their managers aimed at enhancing the use of fair procedures in allocating outcomes and developing their autonomy-supportive behaviours to improve nurses' work satisfaction, organizational identification and job performance. © 2013 Blackwell Publishing Ltd.

  3. A rational approach to heavy-atom derivative screening

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Joyce, M. Gordon; Radaev, Sergei; Sun, Peter D., E-mail: psun@nih.gov

    2010-04-01

    In order to overcome the difficulties associated with the ‘classical’ heavy-atom derivatization procedure, an attempt has been made to develop a rational crystal-free heavy-atom-derivative screening method and a quick-soak derivatization procedure which allows heavy-atom compound identification. Despite the development in recent times of a range of techniques for phasing macromolecules, the conventional heavy-atom derivatization method still plays a significant role in protein structure determination. However, this method has become less popular in modern high-throughput oriented crystallography, mostly owing to its trial-and-error nature, which often results in lengthy empirical searches requiring large numbers of well diffracting crystals. In addition, the phasingmore » power of heavy-atom derivatives is often compromised by lack of isomorphism or even loss of diffraction. In order to overcome the difficulties associated with the ‘classical’ heavy-atom derivatization procedure, an attempt has been made to develop a rational crystal-free heavy-atom derivative-screening method and a quick-soak derivatization procedure which allows heavy-atom compound identification. The method includes three basic steps: (i) the selection of likely reactive compounds for a given protein and specific crystallization conditions based on pre-defined heavy-atom compound reactivity profiles, (ii) screening of the chosen heavy-atom compounds for their ability to form protein adducts using mass spectrometry and (iii) derivatization of crystals with selected heavy-metal compounds using the quick-soak method to maximize diffraction quality and minimize non-isomorphism. Overall, this system streamlines the process of heavy-atom compound identification and minimizes the problem of non-isomorphism in phasing.« less

  4. Ion chromatography characterization of polysaccharides in ancient wall paintings.

    PubMed

    Colombin, Maria Perla; Ceccarini, Alessio; Carmignani, Alessia

    2002-08-30

    An analytical procedure for the characterisation of polysaccharides and the identification of plant gums in old polychrome samples is described. The procedure is based on hydrolysis with 2 M trifluoroacetic acid assisted by microwaves (20 min, 120 degrees C, 500 W), clean-up of the hydrolysate by an ion-exchange resin, and analysis by high-performance anion-exchange chromatography with pulsed amperometric detection. Using this method the hydrolysis time was reduced to 20 min and the chromatographic separation of seven monosaccharides (fucose, rhamnose, arabinose, galactose, glucose, mannose, xylose) and two uronic acids (galacturonic and glucuronic) was achieved in 40 min. The whole analytical procedure allows sugar determination in plant gums at picomole levels, with an average recovery of 72% with an RSD of 8% as tested on arabic gum. The analytical procedure was tested with several raw gums, watercolour samples and reference painting specimens prepared according to old recipes at the Opificio delle Pietre Dure of Florence (Italian Ministry of Cultural Heritage, Italy). All the data collected expressed in relative sugar percentage contents were submitted to principal components analysis for gum identification: five groups were spatially separated and this enabled the identification of arabic, tragacanth, karaya, cherry+ghatty, and guar+locust bean gum. Wall painting samples from Macedonian tombs (Greece) of the 4th-3rd Centuries B.C., processed by the suggested method, showed the presence of a complex paint media mainly consisting of tragacanth and fruit tree gums. Moreover, starch had probably been added to plaster as highlighted by the presence of a huge amount of glucose.

  5. Preharvest food safety.

    PubMed

    Childers, A B; Walsh, B

    1996-07-23

    Preharvest food safety is essential for the protection of our food supply. The production and transport of livestock and poultry play an integral part in the safety of these food products. The goals of this safety assurance include freedom from pathogenic microorganisms, disease, and parasites, and from potentially harmful residues and physical hazards. Its functions should be based on hazard analysis and critical control points from producer to slaughter plant with emphasis on prevention of identifiable hazards rather than on removal of contaminated products. The production goal is to minimize infection and insure freedom from potentially harmful residues and physical hazards. The marketing goal is control of exposure to pathogens and stress. Both groups should have functional hazard analysis and critical control points management programs which include personnel training and certification of producers. These programs must cover production procedures, chemical usage, feeding, treatment practices, drug usage, assembly and transportation, and animal identification. Plans must use risk assessment principles, and the procedures must be defined. Other elements would include preslaughter certification, environmental protection, control of chemical hazards, live-animal drug-testing procedures, and identification of physical hazards.

  6. 12 CFR Appendix J to Part 222 - Interagency Guidelines on Identity Theft Detection, Prevention, and Mitigation

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ..., procedures, and other arrangements that control reasonably foreseeable risks to customers or to the safety... other suspicious activity related to, a covered account; and (5) Notice from customers, victims of... policies and procedures regarding identification and verification set forth in the Customer Identification...

  7. Dangers resulting from DNA profiling of biological materials derived from patients after allogeneic hematopoietic stem cell transplantation (allo-HSCT) with regard to forensic genetic analysis.

    PubMed

    Jacewicz, R; Lewandowski, K; Rupa-Matysek, J; Jędrzejczyk, M; Berent, J

    The study documents the risk that comes with DNA analysis of materials derived from patients after allogeneic hematopoietic stem cell transplantation (allo-HSCT) in forensic genetics. DNA chimerism was studied in 30 patients after allo-HSCT, based on techniques applied in contemporary forensic genetics, i.e. real-time PCR and multiplex PCR-STR with the use of autosomal DNA as well as Y-DNA markers. The results revealed that the DNA profile of the recipient's blood was identical with the donor's in the majority of cases. Therefore, blood analysis can lead to false conclusions in personal identification as well as kinship analysis. An investigation of buccal swabs revealed a mixture of DNA in the majority of recipients. Consequently, personal identification on the basis of stain analysis of the same origin may be impossible. The safest (but not ideal) material turned out to be the hair root. Its analysis based on autosomal DNA revealed 100% of the recipient's profile. However, an analysis based on Y-chromosome markers performed in female allo-HSCT recipients with male donors demonstrated the presence of donor DNA in hair cells - similarly to the blood and buccal swabs. In the light of potential risks arising from DNA profiling of biological materials derived from persons after allotransplantation in judicial aspects, certain procedures were proposed to eliminate such dangers. The basic procedures include abandoning the approach based exclusively on blood collection, both for kinship analysis and personal identification; asking persons who are to be tested about their history of allo-HSCT before sample collection and profile entry in the DNA database, and verification of DNA profiling based on hair follicles in uncertain cases.

  8. System Identification of a Heaving Point Absorber: Design of Experiment and Device Modeling

    DOE PAGES

    Bacelli, Giorgio; Coe, Ryan; Patterson, David; ...

    2017-04-01

    Empirically based modeling is an essential aspect of design for a wave energy converter. These models are used in structural, mechanical and control design processes, as well as for performance prediction. The design of experiments and methods used to produce models from collected data have a strong impact on the quality of the model. This study considers the system identification and model validation process based on data collected from a wave tank test of a model-scale wave energy converter. Experimental design and data processing techniques based on general system identification procedures are discussed and compared with the practices often followedmore » for wave tank testing. The general system identification processes are shown to have a number of advantages. The experimental data is then used to produce multiple models for the dynamics of the device. These models are validated and their performance is compared against one and other. Furthermore, while most models of wave energy converters use a formulation with wave elevation as an input, this study shows that a model using a hull pressure sensor to incorporate the wave excitation phenomenon has better accuracy.« less

  9. System Identification of a Heaving Point Absorber: Design of Experiment and Device Modeling

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Bacelli, Giorgio; Coe, Ryan; Patterson, David

    Empirically based modeling is an essential aspect of design for a wave energy converter. These models are used in structural, mechanical and control design processes, as well as for performance prediction. The design of experiments and methods used to produce models from collected data have a strong impact on the quality of the model. This study considers the system identification and model validation process based on data collected from a wave tank test of a model-scale wave energy converter. Experimental design and data processing techniques based on general system identification procedures are discussed and compared with the practices often followedmore » for wave tank testing. The general system identification processes are shown to have a number of advantages. The experimental data is then used to produce multiple models for the dynamics of the device. These models are validated and their performance is compared against one and other. Furthermore, while most models of wave energy converters use a formulation with wave elevation as an input, this study shows that a model using a hull pressure sensor to incorporate the wave excitation phenomenon has better accuracy.« less

  10. Model identification methodology for fluid-based inerters

    NASA Astrophysics Data System (ADS)

    Liu, Xiaofu; Jiang, Jason Zheng; Titurus, Branislav; Harrison, Andrew

    2018-06-01

    Inerter is the mechanical dual of the capacitor via the force-current analogy. It has the property that the force across the terminals is proportional to their relative acceleration. Compared with flywheel-based inerters, fluid-based forms have advantages of improved durability, inherent damping and simplicity of design. In order to improve the understanding of the physical behaviour of this fluid-based device, especially caused by the hydraulic resistance and inertial effects in the external tube, this work proposes a comprehensive model identification methodology. Firstly, a modelling procedure is established, which allows the topological arrangement of the mechanical networks to be obtained by mapping the damping, inertance and stiffness effects directly to their respective hydraulic counterparts. Secondly, an experimental sequence is followed, which separates the identification of friction, stiffness and various damping effects. Furthermore, an experimental set-up is introduced, where two pressure gauges are used to accurately measure the pressure drop across the external tube. The theoretical models with improved confidence are obtained using the proposed methodology for a helical-tube fluid inerter prototype. The sources of remaining discrepancies are further analysed.

  11. Responsiveness to Intervention: An SLD Determination Resource. Information Digest. Winter 2007

    ERIC Educational Resources Information Center

    Tollefson, Julie M.; Mellard, Daryl F.; McKnight, Melinda A.

    2007-01-01

    The 2004 reauthorization of the Individuals with Disabilities Education Improvement Act (P.L. 108-446) (IDEA 2004) has brought the issue of specific learning disabilities (SLD) identification procedures and criteria to the forefront. Calls for reform are not new and are based on decades of various policy, implementation, and research agendas. The…

  12. Two New Real-Time PCR-based Surveillance Systems for “Candidatus Liberibacter” Species Detection

    USDA-ARS?s Scientific Manuscript database

    We developed two novel surveillance systems for “Candidatus Liberibacter” (CL) species detection and identification. The first system is called “single tube dual primer Taq-Man PCR” (STDP). The procedure involves two sequential rounds of PCR using the CL asiaticus species-specific outer and inner pr...

  13. Recording and cataloging hazards information, revision A

    NASA Technical Reports Server (NTRS)

    Stein, R. J.

    1974-01-01

    A data collection process is described for the purpose of discerning causation factors of accidents, and the establishment of boundaries or controls aimed at mitigating and eliminating accidents. A procedure is proposed that suggests a discipline approach to hazard identification based on energy interrelationships together with an integrated control technique which takes the form of checklists.

  14. Through Their Eyes: Students' Perspectives of a University-Based Enrichment Program--The University for Young People Project

    ERIC Educational Resources Information Center

    Johnsen, Susan K.; Witte, Mary; Robins, Jennifer

    2006-01-01

    Gifted students from economically disadvantaged backgrounds are frequently underrepresented in gifted and talented programs. A number of factors have been identified that appear to limit gifted and talented services to this population: narrow concepts and definitions of giftedness, restrictive identification procedures, inadequate programming, and…

  15. Widening Horizons: A Guide to Organizing Field Trips for Adult Students. Final Report.

    ERIC Educational Resources Information Center

    Lutheran Social Mission Society, Philadelphia, PA. Lutheran Settlement House.

    Based on a successful program for women conducted by Lutheran Settlement House in Philadelphia, this guide outlines step-by-step procedures for conducting educational field trips for students in adult basic education programs. The guide offers suggestions for identification of cultural, historical, and social resources that would provide valuable…

  16. Single-Word Intelligibility in Speakers with Repaired Cleft Palate

    ERIC Educational Resources Information Center

    Whitehill, Tara; Chau, Cynthia

    2004-01-01

    Many speakers with repaired cleft palate have reduced intelligibility, but there are limitations with current procedures for assessing intelligibility. The aim of this study was to construct a single-word intelligibility test for speakers with cleft palate. The test used a multiple-choice identification format, and was based on phonetic contrasts…

  17. A Case Study to Examine Peer Grouping and Aspirant Selection. Professional File. Article 132, Fall 2013

    ERIC Educational Resources Information Center

    D'Allegro, Mary Lou; Zhou, Kai

    2013-01-01

    Peer selection based on the similarity of a couple of institutional parameters, by itself, is insufficient. Several other considerations, including clarity of purpose, alignment of institutional information to that purpose, identification of appropriate statistical procedures, review of preliminary peer sets, and the application of additional…

  18. Identification of the heat transfer coefficient in the two-dimensional model of binary alloy solidification

    NASA Astrophysics Data System (ADS)

    Hetmaniok, Edyta; Hristov, Jordan; Słota, Damian; Zielonka, Adam

    2017-05-01

    The paper presents the procedure for solving the inverse problem for the binary alloy solidification in a two-dimensional space. This is a continuation of some previous works of the authors investigating a similar problem but in the one-dimensional domain. Goal of the problem consists in identification of the heat transfer coefficient on boundary of the region and in reconstruction of the temperature distribution inside the considered region in case when the temperature measurements in selected points of the alloy are known. Mathematical model of the problem is based on the heat conduction equation with the substitute thermal capacity and with the liquidus and solidus temperatures varying in dependance on the concentration of the alloy component. For describing this concentration the Scheil model is used. Investigated procedure involves also the parallelized Ant Colony Optimization algorithm applied for minimizing a functional expressing the error of approximate solution.

  19. Surface-enhanced Raman scattering spectroscopy characterization and identification of foodborne bacteria

    NASA Astrophysics Data System (ADS)

    Liu, Yongliang; Chen, Yud-Ren; Nou, Xiangwu; Chao, Kaunglin

    2007-09-01

    Rapid and routine identification of foodborne bacteria are considerably important, because of bio- / agro- terrorism threats, public health concerns, and economic loss. Conventional, PCR, and immunoassay methods for the detection of bacteria are generally time-consuming, chemical reagent necessary and multi-step procedures. Fast microbial detection requires minimal sample preparation, permits the routine analysis of large numbers of samples with negligible reagent costs, and is easy to operate. Therefore, we have developed silver colloidal nanoparticle based surface-enhanced Raman scattering (SERS) spectroscopy as a potential tool for the rapid and routine detection of E. coli and L. monocytogenes. This study presents the further results of our examination on S. typhimonium, one of the most commonly outbreak bacteria, for the characteristic bands and subsequent identification.

  20. Flexible polyurethane foam modelling and identification of viscoelastic parameters for automotive seating applications

    NASA Astrophysics Data System (ADS)

    Deng, R.; Davies, P.; Bajaj, A. K.

    2003-05-01

    A hereditary model and a fractional derivative model for the dynamic properties of flexible polyurethane foams used in automotive seat cushions are presented. Non-linear elastic and linear viscoelastic properties are incorporated into these two models. A polynomial function of compression is used to represent the non-linear elastic behavior. The viscoelastic property is modelled by a hereditary integral with a relaxation kernel consisting of two exponential terms in the hereditary model and by a fractional derivative term in the fractional derivative model. The foam is used as the only viscoelastic component in a foam-mass system undergoing uniaxial compression. One-term harmonic balance solutions are developed to approximate the steady state response of the foam-mass system to the harmonic base excitation. System identification procedures based on the direct non-linear optimization and a sub-optimal method are formulated to estimate the material parameters. The effects of the choice of the cost function, frequency resolution of data and imperfections in experiments are discussed. The system identification procedures are also applied to experimental data from a foam-mass system. The performances of the two models for data at different compression and input excitation levels are compared, and modifications to the structure of the fractional derivative model are briefly explored. The role of the viscous damping term in both types of model is discussed.

  1. [Photometric determination of butyl "aeroflot" flotation agent (sodium dibutyl dithiophosphate) in the air of work zone and skin washings].

    PubMed

    Dorogova, V B; Kucheriavykh, E I; Sokolova, T V

    1989-01-01

    Photometric procedure of butyl "aeroflot" identification in the work zone air and in wash-out from workers' integument was developed, The procedure was based on the formation of yellow- and orange-dyed copper dibutyl dithiophosphate under butyl "aeroflot" interaction with copper sulphate with the subsequent photometry of dyed solutions for the wavelength of 420 nm in the 10-mm cell. Buffer solution with pH-9.2 was used as an absorbing solution for the workplace air sampling and integument wash-out.

  2. Magnetic scavengers as carriers of analytes for flowing atmospheric pressure afterglow mass spectrometry (FAPA-MS).

    PubMed

    Cegłowski, Michał; Kurczewska, Joanna; Smoluch, Marek; Reszke, Edward; Silberring, Jerzy; Schroeder, Grzegorz

    2015-09-07

    In this paper, a procedure for the preconcentration and transport of mixtures of acids, bases, and drug components to a mass spectrometer using magnetic scavengers is presented. Flowing atmospheric pressure afterglow mass spectrometry (FAPA-MS) was used as an analytical method for identification of the compounds by thermal desorption from the scavengers. The proposed procedure is fast and cheap, and does not involve time-consuming purification steps. The developed methodology can be applied for trapping harmful substances in minute quantities, to transport them to specialized, remotely located laboratories.

  3. Context-Sensitive Markov Models for Peptide Scoring and Identification from Tandem Mass Spectrometry

    PubMed Central

    Grover, Himanshu; Wallstrom, Garrick; Wu, Christine C.

    2013-01-01

    Abstract Peptide and protein identification via tandem mass spectrometry (MS/MS) lies at the heart of proteomic characterization of biological samples. Several algorithms are able to search, score, and assign peptides to large MS/MS datasets. Most popular methods, however, underutilize the intensity information available in the tandem mass spectrum due to the complex nature of the peptide fragmentation process, thus contributing to loss of potential identifications. We present a novel probabilistic scoring algorithm called Context-Sensitive Peptide Identification (CSPI) based on highly flexible Input-Output Hidden Markov Models (IO-HMM) that capture the influence of peptide physicochemical properties on their observed MS/MS spectra. We use several local and global properties of peptides and their fragment ions from literature. Comparison with two popular algorithms, Crux (re-implementation of SEQUEST) and X!Tandem, on multiple datasets of varying complexity, shows that peptide identification scores from our models are able to achieve greater discrimination between true and false peptides, identifying up to ∼25% more peptides at a False Discovery Rate (FDR) of 1%. We evaluated two alternative normalization schemes for fragment ion-intensities, a global rank-based and a local window-based. Our results indicate the importance of appropriate normalization methods for learning superior models. Further, combining our scores with Crux using a state-of-the-art procedure, Percolator, we demonstrate the utility of using scoring features from intensity-based models, identifying ∼4-8 % additional identifications over Percolator at 1% FDR. IO-HMMs offer a scalable and flexible framework with several modeling choices to learn complex patterns embedded in MS/MS data. PMID:23289783

  4. Written Identification of Errors to Learn Professional Procedures in VET

    ERIC Educational Resources Information Center

    Boldrini, Elena; Cattaneo, Alberto

    2013-01-01

    Research has demonstrated that the use of worked-out examples to present errors has great potential for procedural knowledge acquirement. Nevertheless, the identification of errors alone does not directly enhance a deep learning process if it is not adequately scaffolded by written self-explanations. We hypothesised that in learning a professional…

  5. NHEXAS PHASE I MARYLAND STUDY--STANDARD OPERATING PROCEDURE FOR IDENTIFICATION NUMBERS FOR SAMPLES AND FORMS (G03)

    EPA Science Inventory

    The purpose of this SOP is to indicate the proper method for assigning unique Identification Numbers for all samples taken and forms used in the collection of NHEXAS Pilot Studies. All data tracking procedures were built upon these ID numbers. Inspection of these ID numbers pro...

  6. A New Zero-Inflated Negative Binomial Methodology for Latent Category Identification

    ERIC Educational Resources Information Center

    Blanchard, Simon J.; DeSarbo, Wayne S.

    2013-01-01

    We introduce a new statistical procedure for the identification of unobserved categories that vary between individuals and in which objects may span multiple categories. This procedure can be used to analyze data from a proposed sorting task in which individuals may simultaneously assign objects to multiple piles. The results of a synthetic…

  7. Non-intrusive parameter identification procedure user's guide

    NASA Technical Reports Server (NTRS)

    Hanson, G. D.; Jewell, W. F.

    1983-01-01

    Written in standard FORTRAN, NAS is capable of identifying linear as well as nonlinear relations between input and output parameters; the only restriction is that the input/output relation be linear with respect to the unknown coefficients of the estimation equations. The output of the identification algorithm can be specified to be in either the time domain (i.e., the estimation equation coefficients) or in the frequency domain (i.e., a frequency response of the estimation equation). The frame length ("window") over which the identification procedure is to take place can be specified to be any portion of the input time history, thereby allowing the freedom to start and stop the identification procedure within a time history. There also is an option which allows a sliding window, which gives a moving average over the time history. The NAS software also includes the ability to identify several assumed solutions simultaneously for the same or different input data.

  8. Completely automated modal analysis procedure based on the combination of different OMA methods

    NASA Astrophysics Data System (ADS)

    Ripamonti, Francesco; Bussini, Alberto; Resta, Ferruccio

    2018-03-01

    In this work a completely automated output-only Modal Analysis procedure is presented and all its benefits are listed. Based on the merging of different Operational Modal Analysis methods and a statistical approach, the identification process has been improved becoming more robust and giving as results only the real natural frequencies, damping ratios and mode shapes of the system. The effect of the temperature can be taken into account as well, leading to the creation of a better tool for automated Structural Health Monitoring. The algorithm has been developed and tested on a numerical model of a scaled three-story steel building present in the laboratories of Politecnico di Milano.

  9. Forensic identification of CITES protected slimming cactus (Hoodia) using DNA barcoding.

    PubMed

    Gathier, Gerard; van der Niet, Timotheus; Peelen, Tamara; van Vugt, Rogier R; Eurlings, Marcel C M; Gravendeel, Barbara

    2013-11-01

    Slimming cactus (Hoodia), found only in southwestern Africa, is a well-known herbal product for losing weight. Consequently, Hoodia extracts are sought-after worldwide despite a CITES Appendix II status. The failure to eradicate illegal trade is due to problems with detecting and identifying Hoodia using morphological and chemical characters. Our aim was to evaluate the potential of molecular identification of Hoodia based on DNA barcoding. Screening of nrITS1 and psbA-trnH DNA sequences from 26 accessions of Ceropegieae resulted in successful identification, while conventional chemical profiling using DLI-MS led to inaccurate detection and identification of Hoodia. The presence of Hoodia in herbal products was also successfully established using DNA sequences. A validation procedure of our DNA barcoding protocol demonstrated its robustness to changes in PCR conditions. We conclude that DNA barcoding is an effective tool for Hoodia detection and identification which can contribute to preventing illegal trade. © 2013 American Academy of Forensic Sciences.

  10. Gain-Scheduled Fault Tolerance Control Under False Identification

    NASA Technical Reports Server (NTRS)

    Shin, Jong-Yeob; Belcastro, Christine (Technical Monitor)

    2006-01-01

    An active fault tolerant control (FTC) law is generally sensitive to false identification since the control gain is reconfigured for fault occurrence. In the conventional FTC law design procedure, dynamic variations due to false identification are not considered. In this paper, an FTC synthesis method is developed in order to consider possible variations of closed-loop dynamics under false identification into the control design procedure. An active FTC synthesis problem is formulated into an LMI optimization problem to minimize the upper bound of the induced-L2 norm which can represent the worst-case performance degradation due to false identification. The developed synthesis method is applied for control of the longitudinal motions of FASER (Free-flying Airplane for Subscale Experimental Research). The designed FTC law of the airplane is simulated for pitch angle command tracking under a false identification case.

  11. 42 CFR 493.1232 - Standard: Specimen identification and integrity.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 42 Public Health 5 2010-10-01 2010-10-01 false Standard: Specimen identification and integrity... Nonwaived Testing General Laboratory Systems § 493.1232 Standard: Specimen identification and integrity. The laboratory must establish and follow written policies and procedures that ensure positive identification and...

  12. Development of an ELISA for evaluation of swab recovery efficiencies of bovine serum albumin.

    PubMed

    Sparding, Nadja; Slotved, Hans-Christian; Nicolaisen, Gert M; Giese, Steen B; Elmlund, Jón; Steenhard, Nina R

    2014-01-01

    After a potential biological incident the sampling strategy and sample analysis are crucial for the outcome of the investigation and identification. In this study, we have developed a simple sandwich ELISA based on commercial components to quantify BSA (used as a surrogate for ricin) with a detection range of 1.32-80 ng/mL. We used the ELISA to evaluate different protein swabbing procedures (swabbing techniques and after-swabbing treatments) for two swab types: a cotton gauze swab and a flocked nylon swab. The optimal swabbing procedure for each swab type was used to obtain recovery efficiencies from different surface materials. The surface recoveries using the optimal swabbing procedure ranged from 0-60% and were significantly higher from nonporous surfaces compared to porous surfaces. In conclusion, this study presents a swabbing procedure evaluation and a simple BSA ELISA based on commercial components, which are easy to perform in a laboratory with basic facilities. The data indicate that different swabbing procedures were optimal for each of the tested swab types, and the particular swab preference depends on the surface material to be swabbed.

  13. New approach to measure soil particulate organic matter in intact samples using X-ray computed micro-tomography

    NASA Astrophysics Data System (ADS)

    Kravchenko, Alexandra; Negassa, Wakene; Guber, Andrey; Schmidt, Sonja

    2014-05-01

    Particulate soil organic matter (POM) is biologically and chemically active fraction of soil organic matter. It is a source of many agricultural and ecological benefits, among which are POM's contribution to C sequestration. Most of conventional research methods for studying organic matter dynamics involve measurements conducted on pre-processed i.e., ground and sieved soil samples. Unfortunately, grinding and sieving completely destroys soil structure, the component crucial for soil functioning and C protection. Importance of a better understanding of the role of soil structure and of the physical protection that it provides to soil C cannot be overstated; and analysis of quantities, characteristics, and decomposition rates of POM in soil samples with intact structure is among the key elements of gaining such understanding. However, a marked difficulty hindering the progress in such analyses is a lack of tools for identification and quantitative analysis of POM in intact soil samples. Recent advancement in applications of X-ray computed micro-tomography (μ-CT) to soil science has given an opportunity to conduct such analyses. The objective of the current study is to develop a procedure for identification and quantitative characterization of POM within intact soil samples using X-ray μ-CT images and to test performance of the proposed procedure on a set of multiple intact soil macro-aggregates. We used 16 4-6 mm soil aggregates collected at 0-15 cm depth from a Typic Hapludalf soil at multiple field sites with diverse agricultural management history. The aggregates have been scanned at SIMBIOS Centre, Dundee, Scotland at 10 micron resolution. POM was determined from the aggregate images using the developed procedure. The procedure was based on combining image pre-processing steps with discriminant analysis classification. The first component of the procedure consisted of image pre-processing steps based on the range of gray values (GV) along with shape and size of POM pieces. That was followed by discriminant analysis conducted using statistical and geostatistical characteristics of POM pieces. POM identified in the intact individual soil aggregates using the proposed procedure was in good agreement with POM measured in the studied aggregates using conventional lab method (R2=0.75). Of particular importance for accurate identification of POM in the images was the information on spatial characteristics of POM's GVs. Since this is the first attempt of POM determination, future work will be needed to explore how the proposed procedure performs under a variety of potentially influential factors, such as POM's origin and decomposition stage, X-ray scanning settings, image filtering and segmentation methods.

  14. Incorporation of Radio Frequency Identification Tag in Dentures to Facilitate Recognition and Forensic Human Identification

    PubMed Central

    Nuzzolese, E; Marcario, V; Di Vella, G

    2010-01-01

    Forensic identification using odontology is based on the comparison of ante-mortem and post mortem dental records. The insertion of a radio frequency identification (RFId) tag into dentures could be used as an aid to identify decomposed bodies, by storing personal identification data in a small transponder that can be radio-transmitted to a reader connected to a computer. A small passive, 12 x 2,1 mm, read-only RFId-tag was incorporated into the manufacture of three trial complete upper dentures and tested for a signal. The aim of this article is to demonstrate the feasibility of manufacturing such a dental prosthesis, the technical protocols for its implantation in the denture resin and its working principles. Future research and tests are required in order to verify human compatibility of the tagged denture and also to evaluate any potential deterioration in strength when subjected to high temperatures, or for damage resulting from everyday wear and tear. It should also be able to withstand the extreme conditions resulting from major accidents or mass disasters and procedures used to perform a forensic identification. PMID:20657641

  15. Systematic Internal Transcribed Spacer Sequence Analysis for Identification of Clinical Mold Isolates in Diagnostic Mycology: a 5-Year Study▿ †

    PubMed Central

    Ciardo, Diana E.; Lucke, Katja; Imhof, Alex; Bloemberg, Guido V.; Böttger, Erik C.

    2010-01-01

    The implementation of internal transcribed spacer (ITS) sequencing for routine identification of molds in the diagnostic mycology laboratory was analyzed in a 5-year study. All mold isolates (n = 6,900) recovered in our laboratory from 2005 to 2009 were included in this study. According to a defined work flow, which in addition to troublesome phenotypic identification takes clinical relevance into account, 233 isolates were subjected to ITS sequence analysis. Sequencing resulted in successful identification for 78.6% of the analyzed isolates (57.1% at species level, 21.5% at genus level). In comparison, extended in-depth phenotypic characterization of the isolates subjected to sequencing achieved taxonomic assignment for 47.6% of these, with a mere 13.3% at species level. Optimization of DNA extraction further improved the efficacy of molecular identification. This study is the first of its kind to testify to the systematic implementation of sequence-based identification procedures in the routine workup of mold isolates in the diagnostic mycology laboratory. PMID:20573873

  16. Neural network modeling of nonlinear systems based on Volterra series extension of a linear model

    NASA Technical Reports Server (NTRS)

    Soloway, Donald I.; Bialasiewicz, Jan T.

    1992-01-01

    A Volterra series approach was applied to the identification of nonlinear systems which are described by a neural network model. A procedure is outlined by which a mathematical model can be developed from experimental data obtained from the network structure. Applications of the results to the control of robotic systems are discussed.

  17. Aeroservoelastic Uncertainty Model Identification from Flight Data

    NASA Technical Reports Server (NTRS)

    Brenner, Martin J.

    2001-01-01

    Uncertainty modeling is a critical element in the estimation of robust stability margins for stability boundary prediction and robust flight control system development. There has been a serious deficiency to date in aeroservoelastic data analysis with attention to uncertainty modeling. Uncertainty can be estimated from flight data using both parametric and nonparametric identification techniques. The model validation problem addressed in this paper is to identify aeroservoelastic models with associated uncertainty structures from a limited amount of controlled excitation inputs over an extensive flight envelope. The challenge to this problem is to update analytical models from flight data estimates while also deriving non-conservative uncertainty descriptions consistent with the flight data. Multisine control surface command inputs and control system feedbacks are used as signals in a wavelet-based modal parameter estimation procedure for model updates. Transfer function estimates are incorporated in a robust minimax estimation scheme to get input-output parameters and error bounds consistent with the data and model structure. Uncertainty estimates derived from the data in this manner provide an appropriate and relevant representation for model development and robust stability analysis. This model-plus-uncertainty identification procedure is applied to aeroservoelastic flight data from the NASA Dryden Flight Research Center F-18 Systems Research Aircraft.

  18. 40 CFR 265.11 - Identification number.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 40 Protection of Environment 27 2013-07-01 2013-07-01 false Identification number. 265.11 Section... FACILITIES General Facility Standards § 265.11 Identification number. Every facility owner or operator must apply to EPA for an EPA identification number in accordance with the EPA notification procedures (45 FR...

  19. 40 CFR 265.11 - Identification number.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 40 Protection of Environment 26 2011-07-01 2011-07-01 false Identification number. 265.11 Section... FACILITIES General Facility Standards § 265.11 Identification number. Every facility owner or operator must apply to EPA for an EPA identification number in accordance with the EPA notification procedures (45 FR...

  20. 40 CFR 265.11 - Identification number.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 40 Protection of Environment 26 2014-07-01 2014-07-01 false Identification number. 265.11 Section... FACILITIES General Facility Standards § 265.11 Identification number. Every facility owner or operator must apply to EPA for an EPA identification number in accordance with the EPA notification procedures (45 FR...

  1. 40 CFR 265.11 - Identification number.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 40 Protection of Environment 27 2012-07-01 2012-07-01 false Identification number. 265.11 Section... FACILITIES General Facility Standards § 265.11 Identification number. Every facility owner or operator must apply to EPA for an EPA identification number in accordance with the EPA notification procedures (45 FR...

  2. 40 CFR 265.11 - Identification number.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 40 Protection of Environment 25 2010-07-01 2010-07-01 false Identification number. 265.11 Section... FACILITIES General Facility Standards § 265.11 Identification number. Every facility owner or operator must apply to EPA for an EPA identification number in accordance with the EPA notification procedures (45 FR...

  3. Issues and Recommendations: A Report of the Learning Disabilities Task Force on Definition, Criteria and Identification Procedures.

    ERIC Educational Resources Information Center

    Iowa State Dept. of Education, Des Moines. Bureau of Special Education.

    The report, developed by a special Iowa task force, examined issues of definition, criteria, and identification procedures for learning disabilities as a point of departure for the examination of current practices affecting learning disabled students in Iowa. The committee's working definintion of learning disabilities is presented as a basis for…

  4. Preschoolers' Person Description and Identification Accuracy: A Comparison of the Simultaneous and Elimination Lineup Procedures

    ERIC Educational Resources Information Center

    Pozzulo, Joanna D.; Dempsey, Julie; Crescini, Charmagne

    2009-01-01

    Preschoolers' (3- to 6-year-olds) person description and identification abilities were examined using the simultaneous and elimination lineup procedures. Participants (N = 100) were exposed to a 20-minute mask-making session conducted by a female confederate who acted as the mask-making teacher. After a brief delay (20 min), participants were…

  5. Standards-Based Procedural Phenotyping: The Arden Syntax on i2b2.

    PubMed

    Mate, Sebastian; Castellanos, Ixchel; Ganslandt, Thomas; Prokosch, Hans-Ulrich; Kraus, Stefan

    2017-01-01

    Phenotyping, or the identification of patient cohorts, is a recurring challenge in medical informatics. While there are open source tools such as i2b2 that address this problem by providing user-friendly querying interfaces, these platforms lack semantic expressiveness to model complex phenotyping algorithms. The Arden Syntax provides procedural programming language construct, designed specifically for medical decision support and knowledge transfer. In this work, we investigate how language constructs of the Arden Syntax can be used for generic phenotyping. We implemented a prototypical tool to integrate i2b2 with an open source Arden execution environment. To demonstrate the applicability of our approach, we used the tool together with an Arden-based phenotyping algorithm to derive statistics about ICU-acquired hypernatremia. Finally, we discuss how the combination of i2b2's user-friendly cohort pre-selection and Arden's procedural expressiveness could benefit phenotyping.

  6. Molecular tools for cryptic Candida species identification with applications in a clinical laboratory.

    PubMed

    Gamarra, Soledad; Dudiuk, Catiana; Mancilla, Estefanía; Vera Garate, María Verónica; Guerrero, Sergio; Garcia-Effron, Guillermo

    2013-01-01

    Candida spp. includes more than 160 species but only 20 species pose clinical problems. C. albicans and C. parapsilosis account for more than 75% of all the fungemias worldwide. In 1995 and 2005, one C. albicans and two C. parapsilosis-related species were described, respectively. Using phenotypic traits, the identification of these newly described species is inconclusive or impossible. Thus, molecular-based procedures are mandatory. In the proposed educational experiment we have adapted different basic molecular biology techniques designed to identify these species including PCR, multiplex PCR, PCR-based restriction endonuclease analysis and nuclear ribosomal RNA amplification. During the classes, students acquired the ability to search and align gene sequences, design primers, and use bioinformatics software. Also, in the performed experiments, fungal molecular taxonomy concepts were introduced and the obtained results demonstrated that classic identification (phenotypic) in some cases needs to be complemented with molecular-based techniques. As a conclusion we can state that we present an inexpensive and well accepted group of classes involving important concepts that can be recreated in any laboratory. Copyright © 2013 International Union of Biochemistry and Molecular Biology, Inc.

  7. Identification of Anisotropic Criteria for Stratified Soil Based on Triaxial Tests Results

    NASA Astrophysics Data System (ADS)

    Tankiewicz, Matylda; Kawa, Marek

    2017-09-01

    The paper presents the identification methodology of anisotropic criteria based on triaxial test results. The considered material is varved clay - a sedimentary soil occurring in central Poland which is characterized by the so-called "layered microstructure". The strength examination outcomes were identified by standard triaxial tests. The results include the estimated peak strength obtained for a wide range of orientations and confining pressures. Two models were chosen as potentially adequate for the description of the tested material, namely Pariseau and its conjunction with the Jaeger weakness plane. Material constants were obtained by fitting the model to the experimental results. The identification procedure is based on the least squares method. The optimal values of parameters are searched for between specified bounds by sequentially decreasing the distance between points and reducing the length of the searched range. For both considered models the optimal parameters have been obtained. The comparison of theoretical and experimental results as well as the assessment of the suitability of selected criteria for the specified range of confining pressures are presented.

  8. Damage detection in composite panels based on mode-converted Lamb waves sensed using 3D laser scanning vibrometer

    NASA Astrophysics Data System (ADS)

    Pieczonka, Łukasz; Ambroziński, Łukasz; Staszewski, Wiesław J.; Barnoncel, David; Pérès, Patrick

    2017-12-01

    This paper introduces damage identification approach based on guided ultrasonic waves and 3D laser Doppler vibrometry. The method is based on the fact that the symmetric and antisymmetric Lamb wave modes differ in amplitude of the in-plane and out-of-plane vibrations. Moreover, the modes differ also in group velocities and normally they are well separated in time. For a given time window both modes can occur simultaneously only close to the wave source or to a defect that leads to mode conversion. By making the comparison between the in-plane and out-of-plane wave vector components the detection of mode conversion is possible, allowing for superior and reliable damage detection. Experimental verification of the proposed damage identification procedure is performed on fuel tank elements of Reusable Launch Vehicles designed for space exploration. Lamb waves are excited using low-profile, surface-bonded piezoceramic transducers and 3D scanning laser Doppler vibrometer is used to characterize the Lamb wave propagation field. The paper presents theoretical background of the proposed damage identification technique as well as experimental arrangements and results.

  9. A Review of Conventional PCR Assays for the Detection of Selected Phytopathogens of Wheat.

    PubMed

    Kuzdraliński, Adam; Kot, Anna; Szczerba, Hubert; Nowak, Michał; Muszyńska, Marta

    2017-01-01

    Infection of phyllosphere (stems, leaves, husks, and grains) by pathogenic fungi reduces the wheat yield and grain quality. Detection of the main wheat pathogenic fungi provides information about species composition and allows effective and targeted plant treatment. Since conventional procedures for the detection of these organisms are unreliable and time consuming, diagnostic DNA-based methods are required. Nucleic acid amplification technologies are independent of the morphological and biochemical characteristics of fungi. Microorganisms do not need to be cultured. Therefore, a number of PCR-based methodologies have been developed for the identification of key pathogenic fungi, such as Fusarium spp., Puccinia spp., Zymoseptoria tritici, Parastagonospora nodorum, Blumeria graminis f. sp. tritici, and Pyrenophora tritici-repentis. This article reviews frequently used DNA regions for fungus identification and discusses already known PCR assays for detection of the aforementioned wheat pathogens. We demonstrate that PCR-based wheat pathogen identification assays require further research. In particular, the number of diagnostic tests for Fusarium graminearum, Puccinia spp., and P. tritici-repentis are insufficient. © 2017 S. Karger AG, Basel.

  10. Application Of The Wigner-Ville Distribution To The Identification Of Machine Noise

    NASA Astrophysics Data System (ADS)

    Boashash, Boualem; O'Shea, Peter

    1988-02-01

    The theory of signal detection using the Wigner-Ville Distribution (WVD) and the Cross Wagner-Ville Distribution (XWVD) is reviewed, and applied to the signaturing, detection, and identification of some specific machine sounds - the individual cylinder firings of a marine engine. For this task, a 4 step procedure has been devised. The Autocorrelation Function (ACF) is first employed for ascertaining the number of engine cylinders and the firing rate of the engine. Cross-correlation techniques are then used for detecting the occurrence of cylinder firing events. This is followed by the use WVD and XWVD based analyses to produce high resolution Time-Frequency signatures, and finally 2D correlations are employed for identification of the cylinders. The proposed methodology is applied to real data.

  11. 40 CFR 264.11 - Identification number.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 40 Protection of Environment 25 2010-07-01 2010-07-01 false Identification number. 264.11 Section... Facility Standards § 264.11 Identification number. Every facility owner or operator must apply to EPA for an EPA identification number in accordance with the EPA notification procedures (45 FR 12746). [45 FR...

  12. 20 CFR 422.112 - Employer identification numbers.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... 20 Employees' Benefits 2 2012-04-01 2012-04-01 false Employer identification numbers. 422.112... Procedures § 422.112 Employer identification numbers. (a) General. Most employers are required by section...)-1 to obtain an employer identification number (EIN) and to include it on wage reports filed with SSA...

  13. 20 CFR 422.112 - Employer identification numbers.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 20 Employees' Benefits 2 2014-04-01 2014-04-01 false Employer identification numbers. 422.112... Procedures § 422.112 Employer identification numbers. (a) General. Most employers are required by section...)-1 to obtain an employer identification number (EIN) and to include it on wage reports filed with SSA...

  14. 40 CFR 264.11 - Identification number.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 40 Protection of Environment 27 2012-07-01 2012-07-01 false Identification number. 264.11 Section... Facility Standards § 264.11 Identification number. Every facility owner or operator must apply to EPA for an EPA identification number in accordance with the EPA notification procedures (45 FR 12746). [45 FR...

  15. 40 CFR 264.11 - Identification number.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 40 Protection of Environment 26 2014-07-01 2014-07-01 false Identification number. 264.11 Section... Facility Standards § 264.11 Identification number. Every facility owner or operator must apply to EPA for an EPA identification number in accordance with the EPA notification procedures (45 FR 12746). [45 FR...

  16. 40 CFR 264.11 - Identification number.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 40 Protection of Environment 27 2013-07-01 2013-07-01 false Identification number. 264.11 Section... Facility Standards § 264.11 Identification number. Every facility owner or operator must apply to EPA for an EPA identification number in accordance with the EPA notification procedures (45 FR 12746). [45 FR...

  17. 40 CFR 264.11 - Identification number.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 40 Protection of Environment 26 2011-07-01 2011-07-01 false Identification number. 264.11 Section... Facility Standards § 264.11 Identification number. Every facility owner or operator must apply to EPA for an EPA identification number in accordance with the EPA notification procedures (45 FR 12746). [45 FR...

  18. A Teaching Exercise for the Identification of Bacteria Using An Interactive Computer Program.

    ERIC Educational Resources Information Center

    Bryant, Trevor N.; Smith, John E.

    1979-01-01

    Describes an interactive Fortran computer program which provides an exercise in the identification of bacteria. Provides a way of enhancing a student's approach to systematic bacteriology and numerical identification procedures. (Author/MA)

  19. Safe procedure development to manage hazardous drugs in the workplace.

    PubMed

    Gaspar Carreño, Marisa; Achau Muñoz, Rubén; Torrico Martín, Fátima; Agún Gonzalez, Juan José; Sanchez Santos, Jose Cristobal; Cercos Lletí, Ana Cristina; Ramos Orozco, Pedro

    2017-03-01

    To develop a safety working procedure for the employees in the Intermutual Hospital de Levante (HIL) in those areas of activity that deal with the handling of hazardous drugs (MP). The procedure was developed in six phases: 1) hazard definition; 2) definition and identification of processes and development of general correct work practices about hazardous drugs' selection and special handling; 3) detection, selection and set of specific recommendations to handle with hazardous drugs during the processes of preparation and administration included in the hospital GFT; 4) categorization of risk during the preparation/administration and development of an identification system; 5) information and training of professionals; 6) implementation of the identification measures and prevention guidelines. Six processes were detected handling HD. During those processes, thirty HD were identified included in the hospital GFT and a safer alternative was found for 6 of them. The HD were classified into 4 risk categories based on those measures to be taken during the preparation and administration of each of them. The development and implementation of specific safety-work processes dealing with medication handling, allows hospital managers to accomplish effectively with their legal obligations about the area of prevention and provides healthcare professional staff with the adequate techniques and safety equipment to avoid possible dangers and risks of some drugs. Copyright AULA MEDICA EDICIONES 2014. Published by AULA MEDICA. All rights reserved.

  20. A study of methods to predict and measure the transmission of sound through the walls of light aircraft

    NASA Technical Reports Server (NTRS)

    Bernhard, R. J.; Bolton, J. S.; Gardner, B.; Mickol, J.; Mollo, C.; Bruer, C.

    1986-01-01

    Progress was made in the following areas: development of a numerical/empirical noise source identification procedure using bondary element techniques; identification of structure-borne noise paths using structural intensity and finite element methods; development of a design optimization numerical procedure to be used to study active noise control in three-dimensional geometries; measurement of dynamic properties of acoustical foams and incorporation of these properties in models governing three-dimensional wave propagation in foams; and structure-borne sound path identification by use of the Wigner distribution.

  1. A signal-detection-based diagnostic-feature-detection model of eyewitness identification.

    PubMed

    Wixted, John T; Mickes, Laura

    2014-04-01

    The theoretical understanding of eyewitness identifications made from a police lineup has long been guided by the distinction between absolute and relative decision strategies. In addition, the accuracy of identifications associated with different eyewitness memory procedures has long been evaluated using measures like the diagnosticity ratio (the correct identification rate divided by the false identification rate). Framed in terms of signal-detection theory, both the absolute/relative distinction and the diagnosticity ratio are mainly relevant to response bias while remaining silent about the key issue of diagnostic accuracy, or discriminability (i.e., the ability to tell the difference between innocent and guilty suspects in a lineup). Here, we propose a signal-detection-based model of eyewitness identification, one that encourages the use of (and helps to conceptualize) receiver operating characteristic (ROC) analysis to measure discriminability. Recent ROC analyses indicate that the simultaneous presentation of faces in a lineup yields higher discriminability than the presentation of faces in isolation, and we propose a diagnostic feature-detection hypothesis to account for that result. According to this hypothesis, the simultaneous presentation of faces allows the eyewitness to appreciate that certain facial features (viz., those that are shared by everyone in the lineup) are non-diagnostic of guilt. To the extent that those non-diagnostic features are discounted in favor of potentially more diagnostic features, the ability to discriminate innocent from guilty suspects will be enhanced.

  2. Incomplete data based parameter identification of nonlinear and time-variant oscillators with fractional derivative elements

    NASA Astrophysics Data System (ADS)

    Kougioumtzoglou, Ioannis A.; dos Santos, Ketson R. M.; Comerford, Liam

    2017-09-01

    Various system identification techniques exist in the literature that can handle non-stationary measured time-histories, or cases of incomplete data, or address systems following a fractional calculus modeling. However, there are not many (if any) techniques that can address all three aforementioned challenges simultaneously in a consistent manner. In this paper, a novel multiple-input/single-output (MISO) system identification technique is developed for parameter identification of nonlinear and time-variant oscillators with fractional derivative terms subject to incomplete non-stationary data. The technique utilizes a representation of the nonlinear restoring forces as a set of parallel linear sub-systems. In this regard, the oscillator is transformed into an equivalent MISO system in the wavelet domain. Next, a recently developed L1-norm minimization procedure based on compressive sensing theory is applied for determining the wavelet coefficients of the available incomplete non-stationary input-output (excitation-response) data. Finally, these wavelet coefficients are utilized to determine appropriately defined time- and frequency-dependent wavelet based frequency response functions and related oscillator parameters. Several linear and nonlinear time-variant systems with fractional derivative elements are used as numerical examples to demonstrate the reliability of the technique even in cases of noise corrupted and incomplete data.

  3. Crystal identification for a dual-layer-offset LYSO based PET system via Lu-176 background radiation and mean shift algorithm

    NASA Astrophysics Data System (ADS)

    Wei, Qingyang; Ma, Tianyu; Xu, Tianpeng; Zeng, Ming; Gu, Yu; Dai, Tiantian; Liu, Yaqiang

    2018-01-01

    Modern positron emission tomography (PET) detectors are made from pixelated scintillation crystal arrays and readout by Anger logic. The interaction position of the gamma-ray should be assigned to a crystal using a crystal position map or look-up table. Crystal identification is a critical procedure for pixelated PET systems. In this paper, we propose a novel crystal identification method for a dual-layer-offset LYSO based animal PET system via Lu-176 background radiation and mean shift algorithm. Single photon event data of the Lu-176 background radiation are acquired in list-mode for 3 h to generate a single photon flood map (SPFM). Coincidence events are obtained from the same data using time information to generate a coincidence flood map (CFM). The CFM is used to identify the peaks of the inner layer using the mean shift algorithm. The response of the inner layer is deducted from the SPFM by subtracting CFM. Then, the peaks of the outer layer are also identified using the mean shift algorithm. The automatically identified peaks are manually inspected by a graphical user interface program. Finally, a crystal position map is generated using a distance criterion based on these peaks. The proposed method is verified on the animal PET system with 48 detector blocks on a laptop with an Intel i7-5500U processor. The total runtime for whole system peak identification is 67.9 s. Results show that the automatic crystal identification has 99.98% and 99.09% accuracy for the peaks of the inner and outer layers of the whole system respectively. In conclusion, the proposed method is suitable for the dual-layer-offset lutetium based PET system to perform crystal identification instead of external radiation sources.

  4. Integrating field methodology and web-based data collection to assess the reliability of the Alcohol Use Disorders Identification Test (AUDIT).

    PubMed

    Celio, Mark A; Vetter-O'Hagen, Courtney S; Lisman, Stephen A; Johansen, Gerard E; Spear, Linda P

    2011-12-01

    Field methodologies offer a unique opportunity to collect ecologically valid data on alcohol use and its associated problems within natural drinking environments. However, limitations in follow-up data collection methods have left unanswered questions regarding the psychometric properties of field-based measures. The aim of the current study is to evaluate the reliability of self-report data collected in a naturally occurring environment - as indexed by the Alcohol Use Disorders Identification Test (AUDIT) - compared to self-report data obtained through an innovative web-based follow-up procedure. Individuals recruited outside of bars (N=170; mean age=21; range 18-32) provided a BAC sample and completed a self-administered survey packet that included the AUDIT. BAC feedback was provided anonymously through a dedicated web page. Upon sign in, follow-up participants (n=89; 52%) were again asked to complete the AUDIT before receiving their BAC feedback. Reliability analyses demonstrated that AUDIT scores - both continuous and dichotomized at the standard cut-point - were stable across field- and web-based administrations. These results suggest that self-report data obtained from acutely intoxicated individuals in naturally occurring environments are reliable when compared to web-based data obtained after a brief follow-up interval. Furthermore, the results demonstrate the feasibility, utility, and potential of integrating field methods and web-based data collection procedures. Copyright © 2011 Elsevier Ireland Ltd. All rights reserved.

  5. 40 CFR Appendix Xviii to Part 86 - Statistical Outlier Identification Procedure for Light-Duty Vehicles and Light Light-Duty Trucks...

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 40 Protection of Environment 20 2012-07-01 2012-07-01 false Statistical Outlier Identification Procedure for Light-Duty Vehicles and Light Light-Duty Trucks Certifying to the Provisions of Part 86... (CONTINUED) AIR PROGRAMS (CONTINUED) CONTROL OF EMISSIONS FROM NEW AND IN-USE HIGHWAY VEHICLES AND ENGINES...

  6. 40 CFR Appendix Xviii to Part 86 - Statistical Outlier Identification Procedure for Light-Duty Vehicles and Light Light-Duty Trucks...

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 40 Protection of Environment 20 2013-07-01 2013-07-01 false Statistical Outlier Identification Procedure for Light-Duty Vehicles and Light Light-Duty Trucks Certifying to the Provisions of Part 86... (CONTINUED) AIR PROGRAMS (CONTINUED) CONTROL OF EMISSIONS FROM NEW AND IN-USE HIGHWAY VEHICLES AND ENGINES...

  7. APPLYING TOXICITY IDENTIFICATION PROCEDURES TO FIELD COLLECTED SEDIMENTS

    EPA Science Inventory

    Identification of specific causes of sediment toxicity can allow for much more focused risk assessment and management decision making. We have been developing toxicity identification evaluation (TIE) methods for contaminated sediments and focusing on three toxicant groups (ammoni...

  8. Ancient DNA in historical parchments - identifying a procedure for extraction and amplification of genetic material.

    PubMed

    Lech, T

    2016-05-06

    Historical parchments in the form of documents, manuscripts, books, or letters, make up a large portion of cultural heritage collections. Their priceless historical value is associated with not only their content, but also the information hidden in the DNA deposited on them. Analyses of ancient DNA (aDNA) retrieved from parchments can be used in various investigations, including, but not limited to, studying their authentication, tracing the development of the culture, diplomacy, and technology, as well as obtaining information on the usage and domestication of animals. This article proposes and verifies a procedure for aDNA recovery from historical parchments and its appropriate preparation for further analyses. This study involved experimental selection of an aDNA extraction method with the highest efficiency and quality of extracted genetic material, from among the multi-stage phenol-chloroform extraction methods, and the modern, column-based techniques that use selective DNA-binding membranes. Moreover, current techniques to amplify entire genetic material were questioned, and the possibility of using mitochondrial DNA for species identification was analyzed. The usefulness of the proposed procedure was successfully confirmed in identification tests of historical parchments dating back to the 13-16th century AD.

  9. A critical review of variables affecting the accuracy and false-negative rate of sentinel node biopsy procedures in early breast cancer.

    PubMed

    Vijayakumar, Vani; Boerner, Philip S; Jani, Ashesh B; Vijayakumar, Srinivasan

    2005-05-01

    Radionuclide sentinel lymph node localization and biopsy is a staging procedure that is being increasingly used to evaluate patients with invasive breast cancer who have clinically normal axillary nodes. The most important prognostic indicator in patients with invasive breast cancer is the axillary node status, which must also be known for correct staging, and influences the selection of adjuvant therapies. The accuracy of sentinel lymph node localization depends on a number of factors, including the injection method, the operating surgeon's experience and the hospital setting. The efficacy of sentinel lymph node mapping can be determined by two measures: the sentinel lymph node identification rate and the false-negative rate. Of these, the false-negative rate is the most important, based on a review of 92 studies. As sentinel lymph node procedures vary widely, nuclear medicine physicians and radiologists must be acquainted with the advantages and disadvantages of the various techniques. In this review, the factors that influence the success of different techniques are examined, and studies which have investigated false-negative rates and/or sentinel lymph node identification rates are summarized.

  10. [Rapid identification of microorganisms by mass spectrometry in a blood culture system. Comparison of two procedures].

    PubMed

    Cattani, María E; Posse, Tamara; Hermes, Ricardo L; Kaufman, Sara C

    2015-01-01

    Rapid identification of microorganisms is critical in hospitalized infected patients. Blood culture is currently the gold standard for detecting and identifying microorganisms causing bacteremia or sepsis. The introduction of mass spectrometry by matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF MS) in microbiology laboratories, especially in microorganisms growing in blood culture bottles, provides rapid identification. This study evaluates the performance of the Maldi Sepsityper Biotyper procedure (hereinafter, MS) compared to that of an in-home method (hereinafter, HF). Eight hundred and forty (840) positive blood culture bottles were processed using the HF procedure, 542 of which were also processed using MS. The organisms were identified in 670 (79.76%) and 391 (72.14%) bottles respectively (p = 0,0013). This study demonstrates the effectiveness of both procedures for identifying microorganisms directly from positive blood culture bottles. However, the HF procedure proved to be more effective than MS, especially in the presence of Gram positive organisms. Copyright © 2015 Asociación Argentina de Microbiología. Publicado por Elsevier España, S.L.U. All rights reserved.

  11. Cognitive Dissonance and Pediatric Procedural Pain Management: A Concept Clarification.

    PubMed

    Bice, April A

    2018-06-01

    Pediatric nurses have often reported that pain management is a vital part of patient care. Evidence, however, suggests pediatric procedural pain treatments are often underused. Cognitive dissonance, the mental conflict leading to unpleasant thoughts and or feelings, may be related to this evidence-based gap found between what pediatric nurses claim about procedural pain management (that it is important) and what they actually do (underutilize pain treatments). The purpose of this manuscript is to clarify and further develop the concept of cognitive dissonance in terms of its relationship to nurses' mental struggles with underutilization of pediatric procedural pain treatments. A more relevant and extended definition of cognitive dissonance is presented. The concept of cognitive dissonance was examined using Rodgers' evolutionary concept analysis approach/framework. Analysis Methods: Through a six-step process of concept identification, setting and sample identification, data collection, data analysis, and future implication discussion, a more accurate and representative definition of cognitive dissonance is described. Databases used included CINAHL, Google Scholar, PsycINFO, ERIC, and PubMed. Seminal, recent, and relevant works were included in the review to adequately develop and clarify the concept. Procedural pain management breech among pediatric nurses is proposed to occur before the mental conflict produced. The unpleasant mental conflict created after the breech is followed by the nurse's determination to reduce mental conflict through attitude change followed by cognition change, which more closely reflects his or her behavior. Copyright © 2017 American Society for Pain Management Nursing. Published by Elsevier Inc. All rights reserved.

  12. Screening of antifungal azole drugs and agrochemicals with an adapted alamarBlue-based assay demonstrates antibacterial activity of croconazole against Mycobacterium ulcerans.

    PubMed

    Scherr, Nicole; Röltgen, Katharina; Witschel, Matthias; Pluschke, Gerd

    2012-12-01

    An alamarBlue-based growth inhibition assay has been adapted for the thermosensitive and slow-growing pathogen Mycobacterium ulcerans. The standardized test procedure enables medium-throughput screening of preselected compound libraries. Testing of a set of 48 azoles with known antifungal activity led to the identification of an imidazole antifungal displaying an inhibitory dose (ID) of 9 μM for M. ulcerans.

  13. Screening of Antifungal Azole Drugs and Agrochemicals with an Adapted alamarBlue-Based Assay Demonstrates Antibacterial Activity of Croconazole against Mycobacterium ulcerans

    PubMed Central

    Röltgen, Katharina; Witschel, Matthias; Pluschke, Gerd

    2012-01-01

    An alamarBlue-based growth inhibition assay has been adapted for the thermosensitive and slow-growing pathogen Mycobacterium ulcerans. The standardized test procedure enables medium-throughput screening of preselected compound libraries. Testing of a set of 48 azoles with known antifungal activity led to the identification of an imidazole antifungal displaying an inhibitory dose (ID) of 9 μM for M. ulcerans. PMID:23006761

  14. Constitutive error based parameter estimation technique for plate structures using free vibration signatures

    NASA Astrophysics Data System (ADS)

    Guchhait, Shyamal; Banerjee, Biswanath

    2018-04-01

    In this paper, a variant of constitutive equation error based material parameter estimation procedure for linear elastic plates is developed from partially measured free vibration sig-natures. It has been reported in many research articles that the mode shape curvatures are much more sensitive compared to mode shape themselves to localize inhomogeneity. Complying with this idea, an identification procedure is framed as an optimization problem where the proposed cost function measures the error in constitutive relation due to incompatible curvature/strain and moment/stress fields. Unlike standard constitutive equation error based procedure wherein a solution of a couple system is unavoidable in each iteration, we generate these incompatible fields via two linear solves. A simple, yet effective, penalty based approach is followed to incorporate measured data. The penalization parameter not only helps in incorporating corrupted measurement data weakly but also acts as a regularizer against the ill-posedness of the inverse problem. Explicit linear update formulas are then developed for anisotropic linear elastic material. Numerical examples are provided to show the applicability of the proposed technique. Finally, an experimental validation is also provided.

  15. Double-blind photo lineups using actual eyewitnesses: an experimental test of a sequential versus simultaneous lineup procedure.

    PubMed

    Wells, Gary L; Steblay, Nancy K; Dysart, Jennifer E

    2015-02-01

    Eyewitnesses (494) to actual crimes in 4 police jurisdictions were randomly assigned to view simultaneous or sequential photo lineups using laptop computers and double-blind administration. The sequential procedure used in the field experiment mimicked how it is conducted in actual practice (e.g., using a continuation rule, witness does not know how many photos are to be viewed, witnesses resolve any multiple identifications), which is not how most lab experiments have tested the sequential lineup. No significant differences emerged in rates of identifying lineup suspects (25% overall) but the sequential procedure produced a significantly lower rate (11%) of identifying known-innocent lineup fillers than did the simultaneous procedure (18%). The simultaneous/sequential pattern did not significantly interact with estimator variables and no lineup-position effects were observed for either the simultaneous or sequential procedures. Rates of nonidentification were not significantly different for simultaneous and sequential but nonidentifiers from the sequential procedure were more likely to use the "not sure" response option than were nonidentifiers from the simultaneous procedure. Among witnesses who made an identification, 36% (41% of simultaneous and 32% of sequential) identified a known-innocent filler rather than a suspect, indicating that eyewitness performance overall was very poor. The results suggest that the sequential procedure that is used in the field reduces the identification of known-innocent fillers, but the differences are relatively small.

  16. Crop identification technology assessment for remote sensing (CITARS). Volume 6: Data processing at the laboratory for applications of remote sensing

    NASA Technical Reports Server (NTRS)

    Bauer, M. E.; Cary, T. K.; Davis, B. J.; Swain, P. H.

    1975-01-01

    The results of classifications and experiments for the crop identification technology assessment for remote sensing are summarized. Using two analysis procedures, 15 data sets were classified. One procedure used class weights while the other assumed equal probabilities of occurrence for all classes. Additionally, 20 data sets were classified using training statistics from another segment or date. The classification and proportion estimation results of the local and nonlocal classifications are reported. Data also describe several other experiments to provide additional understanding of the results of the crop identification technology assessment for remote sensing. These experiments investigated alternative analysis procedures, training set selection and size, effects of multitemporal registration, spectral discriminability of corn, soybeans, and other, and analyses of aircraft multispectral data.

  17. Methods for parameter identification in oscillatory networks and application to cortical and thalamic 600 Hz activity.

    PubMed

    Leistritz, L; Suesse, T; Haueisen, J; Hilgenfeld, B; Witte, H

    2006-01-01

    Directed information transfer in the human brain occurs presumably by oscillations. As of yet, most approaches for the analysis of these oscillations are based on time-frequency or coherence analysis. The present work concerns the modeling of cortical 600 Hz oscillations, localized within the Brodmann Areas 3b and 1 after stimulation of the nervus medianus, by means of coupled differential equations. This approach leads to the so-called parameter identification problem, where based on a given data set, a set of unknown parameters of a system of ordinary differential equations is determined by special optimization procedures. Some suitable algorithms for this task are presented in this paper. Finally an oscillatory network model is optimally fitted to the data taken from ten volunteers.

  18. Measurement of gamma' precipitates in a nickel-based superalloy using energy-filtered transmission electron microscopy coupled with automated segmenting techniques.

    PubMed

    Tiley, J S; Viswanathan, G B; Shiveley, A; Tschopp, M; Srinivasan, R; Banerjee, R; Fraser, H L

    2010-08-01

    Precipitates of the ordered L1(2) gamma' phase (dispersed in the face-centered cubic or FCC gamma matrix) were imaged in Rene 88 DT, a commercial multicomponent Ni-based superalloy, using energy-filtered transmission electron microscopy (EFTEM). Imaging was performed using the Cr, Co, Ni, Ti and Al elemental L-absorption edges in the energy loss spectrum. Manual and automated segmentation procedures were utilized for identification of precipitate boundaries and measurement of precipitate sizes. The automated region growing technique for precipitate identification in images was determined to measure accurately precipitate diameters. In addition, the region growing technique provided a repeatable method for optimizing segmentation techniques for varying EFTEM conditions. (c) 2010 Elsevier Ltd. All rights reserved.

  19. Catheter-based treatment of coronary artery disease: past, present, and future.

    PubMed

    Holmes, David R; Williams, David O

    2008-08-01

    September 2007 marked the 30-year anniversary of the first human percutaneous coronary intervention, an index event that changed the course of modern-day cardiovascular care. Before that first procedure, adult invasive cardiology focused on diagnostic angiography as well as hemodynamic assessment of structural heart disease. Since that initial procedure, percutaneous coronary intervention has become the most frequently performed coronary revascularization procedure worldwide. Several factors have been responsible for this dramatic paradigm shift, the most prominent being identification of opportunities for technical improvement and the application of innovation and investigation in concert with colleagues, professional societies, and industry. These approaches will continue to be of paramount importance as new technologies are brought to bear on an increasingly broader group of patients with cardiovascular disease.

  20. What the Tweets Say: A Critical Analysis of Twitter Research in Language Learning from 2009 to 2016

    ERIC Educational Resources Information Center

    Hattem, David; Lomicka, Lara

    2016-01-01

    This study presents an overview and critical analysis of the literature related to Twitter and language learning published from 2009 to 2016. Seventeen studies were selected for inclusion based on a four-phase identification procedure, which helped us to identify published studies that resulted in a content analysis of themes in the articles…

  1. Recursive inversion of externally defined linear systems

    NASA Technical Reports Server (NTRS)

    Bach, Ralph E., Jr.; Baram, Yoram

    1988-01-01

    The approximate inversion of an internally unknown linear system, given by its impulse response sequence, by an inverse system having a finite impulse response, is considered. The recursive least squares procedure is shown to have an exact initialization, based on the triangular Toeplitz structure of the matrix involved. The proposed approach also suggests solutions to the problems of system identification and compensation.

  2. Group Connotation in the Analysis of the Images in Motion Used in Television Departments

    ERIC Educational Resources Information Center

    Caldera-Serrano, Jorge

    2010-01-01

    This paper describes a procedure to manage connotations, so that they may be identified in the document databases of television channels. The system is based on Ranganathan's facets, as this is the best tool to describe actions--i.e. the units analysed in television--which enable the identification of the connoted information by introducing or…

  3. Predictability of physiological testing and the role of maturation in talent identification for adolescent team sports.

    PubMed

    Pearson, D T; Naughton, G A; Torode, M

    2006-08-01

    Entrepreneurial marketing of sport increases demands on sport development officers to identify talented individuals for specialist development at the youngest possible age. Talent identification results in the streamlining of resources to produce optimal returns from a sports investment. However, the process of talent identification for team sports is complex and success prediction is imperfect. The aim of this review is to describe existing practices in physiological tests used for talent identification in team sports and discuss the impact of maturity-related differences on the long term outcomes particularly for male participants. Maturation is a major confounding variable in talent identification during adolescence. A myriad of hormonal changes during puberty results in physical and physiological characteristics important for sporting performance. Significant changes during puberty make the prediction of adult performance difficult from adolescent data. Furthermore, for talent identification programs to succeed, valid and reliable testing procedures must be accepted and implemented in a range of performance-related categories. Limited success in scientifically based talent identification is evident in a range of team sports. Genetic advances challenge the ethics of talent identification in adolescent sport. However, the environment remains a significant component of success prediction in sport. Considerations for supporting talented young male athletes are discussed.

  4. RESULTS OF APPLYING TOXICITY IDENTIFICATION PROCEDURES TO FIELD COLLECTED SEDIMENTS

    EPA Science Inventory

    Identification of specific causes of sediment toxicity can allow for much more focused risk assessment and management decision making. We have been developing toxicity identification evaluation TIE) methods for contaminated sediments and are focusing on three toxicant groups (amm...

  5. 47 CFR 90.425 - Station identification.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... MOBILE RADIO SERVICES Operating Requirements § 90.425 Station identification. Stations licensed under... disabled. Permissible alternative identification procedures are as follows: (1) A mobile relay stations call sign may be used to identify the associated control and mobile stations, except in the Public...

  6. 47 CFR 90.425 - Station identification.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... MOBILE RADIO SERVICES Operating Requirements § 90.425 Station identification. Stations licensed under... disabled. Permissible alternative identification procedures are as follows: (1) A mobile relay stations call sign may be used to identify the associated control and mobile stations, except in the Public...

  7. 42 CFR 493.1232 - Standard: Specimen identification and integrity.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... AND HUMAN SERVICES (CONTINUED) STANDARDS AND CERTIFICATION LABORATORY REQUIREMENTS Quality System for Nonwaived Testing General Laboratory Systems § 493.1232 Standard: Specimen identification and integrity. The laboratory must establish and follow written policies and procedures that ensure positive identification and...

  8. Development of a Test Facility for Air Revitalization Technology Evaluation

    NASA Technical Reports Server (NTRS)

    Lu, Sao-Dung; Lin, Amy; Campbell, Melissa; Smith, Frederick

    2006-01-01

    An active fault tolerant control (FTC) law is generally sensitive to false identification since the control gain is reconfigured for fault occurrence. In the conventional FTC law design procedure, dynamic variations due to false identification are not considered. In this paper, an FTC synthesis method is developed in order to consider possible variations of closed-loop dynamics under false identification into the control design procedure. An active FTC synthesis problem is formulated into an LMI optimization problem to minimize the upper bound of the induced-L2 norm which can represent the worst-case performance degradation due to false identification. The developed synthesis method is applied for control of the longitudinal motions of FASER (Free-flying Airplane for Subscale Experimental Research). The designed FTC law of the airplane is simulated for pitch angle command tracking under a false identification case.

  9. A new surface fractal dimension for displacement mode shape-based damage identification of plate-type structures

    NASA Astrophysics Data System (ADS)

    Shi, Binkai; Qiao, Pizhong

    2018-03-01

    Vibration-based nondestructive testing is an area of growing interest and worthy of exploring new and innovative approaches. The displacement mode shape is often chosen to identify damage due to its local detailed characteristic and less sensitivity to surrounding noise. Requirement for baseline mode shape in most vibration-based damage identification limits application of such a strategy. In this study, a new surface fractal dimension called edge perimeter dimension (EPD) is formulated, from which an EPD-based window dimension locus (EPD-WDL) algorithm for irregularity or damage identification of plate-type structures is established. An analytical notch-type damage model of simply-supported plates is proposed to evaluate notch effect on plate vibration performance; while a sub-domain of notch cases with less effect is selected to investigate robustness of the proposed damage identification algorithm. Then, fundamental aspects of EPD-WDL algorithm in term of notch localization, notch quantification, and noise immunity are assessed. A mathematical solution called isomorphism is implemented to remove false peaks caused by inflexions of mode shapes when applying the EPD-WDL algorithm to higher mode shapes. The effectiveness and practicability of the EPD-WDL algorithm are demonstrated by an experimental procedure on damage identification of an artificially-induced notched aluminum cantilever plate using a measurement system of piezoelectric lead-zirconate (PZT) actuator and scanning laser Doppler vibrometer (SLDV). As demonstrated in both the analytical and experimental evaluations, the new surface fractal dimension technique developed is capable of effectively identifying damage in plate-type structures.

  10. Security Considerations and Recommendations in Computer-Based Testing

    PubMed Central

    Al-Saleem, Saleh M.

    2014-01-01

    Many organizations and institutions around the globe are moving or planning to move their paper-and-pencil based testing to computer-based testing (CBT). However, this conversion will not be the best option for all kinds of exams and it will require significant resources. These resources may include the preparation of item banks, methods for test delivery, procedures for test administration, and last but not least test security. Security aspects may include but are not limited to the identification and authentication of examinee, the risks that are associated with cheating on the exam, and the procedures related to test delivery to the examinee. This paper will mainly investigate the security considerations associated with CBT and will provide some recommendations for the security of these kinds of tests. We will also propose a palm-based biometric authentication system incorporated with basic authentication system (username/password) in order to check the identity and authenticity of the examinee. PMID:25254250

  11. Security considerations and recommendations in computer-based testing.

    PubMed

    Al-Saleem, Saleh M; Ullah, Hanif

    2014-01-01

    Many organizations and institutions around the globe are moving or planning to move their paper-and-pencil based testing to computer-based testing (CBT). However, this conversion will not be the best option for all kinds of exams and it will require significant resources. These resources may include the preparation of item banks, methods for test delivery, procedures for test administration, and last but not least test security. Security aspects may include but are not limited to the identification and authentication of examinee, the risks that are associated with cheating on the exam, and the procedures related to test delivery to the examinee. This paper will mainly investigate the security considerations associated with CBT and will provide some recommendations for the security of these kinds of tests. We will also propose a palm-based biometric authentication system incorporated with basic authentication system (username/password) in order to check the identity and authenticity of the examinee.

  12. Medical Service Clinical Laboratory Procedures--Bacteriology.

    ERIC Educational Resources Information Center

    Department of the Army, Washington, DC.

    This manual presents laboratory procedures for the differentiation and identification of disease agents from clinical materials. Included are procedures for the collection of specimens, preparation of culture media, pure culture methods, cultivation of the microorganisms in natural and simulated natural environments, and procedures in…

  13. Failure Mode Identification Through Clustering Analysis

    NASA Technical Reports Server (NTRS)

    Arunajadai, Srikesh G.; Stone, Robert B.; Tumer, Irem Y.; Clancy, Daniel (Technical Monitor)

    2002-01-01

    Research has shown that nearly 80% of the costs and problems are created in product development and that cost and quality are essentially designed into products in the conceptual stage. Currently, failure identification procedures (such as FMEA (Failure Modes and Effects Analysis), FMECA (Failure Modes, Effects and Criticality Analysis) and FTA (Fault Tree Analysis)) and design of experiments are being used for quality control and for the detection of potential failure modes during the detail design stage or post-product launch. Though all of these methods have their own advantages, they do not give information as to what are the predominant failures that a designer should focus on while designing a product. This work uses a functional approach to identify failure modes, which hypothesizes that similarities exist between different failure modes based on the functionality of the product/component. In this paper, a statistical clustering procedure is proposed to retrieve information on the set of predominant failures that a function experiences. The various stages of the methodology are illustrated using a hypothetical design example.

  14. Toward a better guard of coastal water safety-Microbial distribution in coastal water and their facile detection.

    PubMed

    Xie, Yunxuan; Qiu, Ning; Wang, Guangyi

    2017-05-15

    Prosperous development in marine-based tourism has raised increasing concerns over the sanitary quality of coastal waters with potential microbial contamination. The World Health Organization has set stringent standards over a list of pathogenic microorganisms posing potential threats to people with frequent coastal water exposure and has asked for efficient detection procedures for pathogen facile identification. Inspection of survey events regarding the occurrence of marine pathogens in recreational beaches in recent years has reinforced the need for the development of a rapid identification procedure. In this review, we examine the possibility of recruiting uniform molecular assays to identify different marine pathogens and the feasibility of appropriate biomarkers, including enterochelin biosynthetic genes, for general toxicity assays. The focus is not only on bacterial pathogens but also on other groups of infectious pathogens. The ultimate goal is the development of a handy method to more efficiently and rapidly detect marine pathogens. Copyright © 2017 Elsevier Ltd. All rights reserved.

  15. Electromigration techniques--a fast and economical tool for differentiation of similar strains of microorganisms.

    PubMed

    Horká, Marie; Horký, Jaroslav; Kubesová, Anna; Mazanec, Karel; Matousková, Hana; Slais, Karel

    2010-07-01

    The detection and identification of pathogens currently relies upon a very diverse range of techniques and skills, from traditional cultivation and taxonomic procedures to modern rapid and sensitive diagnostic methods. Real-time PCR is now exploited as a front line diagnostic screening tool in human, animal and plant health as well as bio-security. Nevertheless, new techniques for pathogen identification, particularly of unknown samples, are needed. In this study we propose the combination of electrophoresis-based procedures for the fast differentiation of microorganisms. The method feasibility is proved on the model of seven similar strains of Pseudomonas syringae pathovars from 37 sources, identified by gas chromatography of fatty acid methyl esters. The results from the routine laboratory were compared with results of the combination of the developed capillary and gel electrophoresis as well as mass spectrometry. According to our experiments appropriate combination of electromigration techniques appears to be useful for the fast and economical differentiation of unknown microorganisms.

  16. Manufacturing Phenomena or Preserving Phenomena? Core Issues in the Identification of Peer Social Groups with Social Cognitive Mapping Procedures

    ERIC Educational Resources Information Center

    Farmer, Thomas W.; Xie, Hongling

    2013-01-01

    In this commentary on the "Multiple Meanings of Peer Groups in Social Cognitive Mapping," Thomas W. Farmer and Hongling Xie discuss core issues in the identification of peer social groups in natural settings using the social cognitive mapping (SCM) procedures. Farmer and Xie applaud the authors for their efforts to advance the study of…

  17. Phylogenomics of plant genomes: a methodology for genome-wide searches for orthologs in plants

    PubMed Central

    Conte, Matthieu G; Gaillard, Sylvain; Droc, Gaetan; Perin, Christophe

    2008-01-01

    Background Gene ortholog identification is now a major objective for mining the increasing amount of sequence data generated by complete or partial genome sequencing projects. Comparative and functional genomics urgently need a method for ortholog detection to reduce gene function inference and to aid in the identification of conserved or divergent genetic pathways between several species. As gene functions change during evolution, reconstructing the evolutionary history of genes should be a more accurate way to differentiate orthologs from paralogs. Phylogenomics takes into account phylogenetic information from high-throughput genome annotation and is the most straightforward way to infer orthologs. However, procedures for automatic detection of orthologs are still scarce and suffer from several limitations. Results We developed a procedure for ortholog prediction between Oryza sativa and Arabidopsis thaliana. Firstly, we established an efficient method to cluster A. thaliana and O. sativa full proteomes into gene families. Then, we developed an optimized phylogenomics pipeline for ortholog inference. We validated the full procedure using test sets of orthologs and paralogs to demonstrate that our method outperforms pairwise methods for ortholog predictions. Conclusion Our procedure achieved a high level of accuracy in predicting ortholog and paralog relationships. Phylogenomic predictions for all validated gene families in both species were easily achieved and we can conclude that our methodology outperforms similarly based methods. PMID:18426584

  18. Application of MALDI-TOF mass spectrometry in clinical diagnostic microbiology.

    PubMed

    De Carolis, Elena; Vella, Antonietta; Vaccaro, Luisa; Torelli, Riccardo; Spanu, Teresa; Fiori, Barbara; Posteraro, Brunella; Sanguinetti, Maurizio

    2014-09-12

    Matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF MS) has recently emerged as a powerful technique for identification of microorganisms, changing the workflow of well-established laboratories so that its impact on microbiological diagnostics has been unparalleled. In comparison with conventional identification methods that rely on biochemical tests and require long incubation procedures, MALDI-TOF MS has the advantage of identifying bacteria and fungi directly from colonies grown on culture plates in a few minutes and with simple procedures. Numerous studies on different systems available demonstrate the reliability and accuracy of the method, and new frontiers have been explored besides microbial species level identification, such as direct identification of pathogens from positive blood cultures, subtyping, and drug susceptibility detection.

  19. HIGH-THROUGHPUT IDENTIFICATION OF CATALYTIC REDOX-ACTIVE CYSTEINE RESIDUES

    EPA Science Inventory

    Cysteine (Cys) residues often play critical roles in proteins; however, identification of their specific functions has been limited to case-by-case experimental approaches. We developed a procedure for high-throughput identification of catalytic redox-active Cys in proteins by se...

  20. Identification of Polymers in University Class Experiments.

    ERIC Educational Resources Information Center

    Bowen, Humphry J. M.

    1990-01-01

    The apparatus, reagents, preliminary classification, nomenclature, acquisition, and procedures used in the identification of synthetic polymers are described. Specific tests for the identification of the presence of hydrocarbons, chlorine, fluorine, sulfur, and nitrogen and the absence of halogens and sulfur are discussed. (CW)

  1. Immune-Response Patterns and Next Generation Sequencing Diagnostics for the Detection of Mycoses in Patients with Septic Shock-Results of a Combined Clinical and Experimental Investigation.

    PubMed

    Decker, Sebastian O; Sigl, Annette; Grumaz, Christian; Stevens, Philip; Vainshtein, Yevhen; Zimmermann, Stefan; Weigand, Markus A; Hofer, Stefan; Sohn, Kai; Brenner, Thorsten

    2017-08-18

    Fungi are of increasing importance in sepsis. However, culture-based diagnostic procedures are associated with relevant weaknesses. Therefore, culture- and next-generation sequencing (NGS)-based fungal findings as well as corresponding plasma levels of β-d-glucan, interferon gamma (INF-γ), tumor necrosis factor alpha (TNF-α), interleukin (IL)-2, -4, -6, -10, -17A, and mid-regional proadrenomedullin (MR-proADM) were evaluated in 50 septic patients at six consecutive time points within 28 days after sepsis onset. Furthermore, immune-response patterns during infections with Candida spp. were studied in a reconstituted human epithelium model. In total, 22% ( n = 11) of patients suffered from a fungal infection. An NGS-based diagnostic approach appeared to be suitable for the identification of fungal pathogens in patients suffering from fungemia as well as in patients with negative blood cultures. Moreover, MR-proADM and IL-17A in plasma proved suitable for the identification of patients with a fungal infection. Using RNA-seq., adrenomedullin (ADM) was shown to be a target gene which is upregulated early after an epithelial infection with Candida spp. In summary, an NGS-based diagnostic approach was able to close the diagnostic gap of routinely used culture-based diagnostic procedures, which can be further facilitated by plasmatic measurements of MR-proADM and IL-17A. In addition, ADM was identified as an early target gene in response to epithelial infections with Candida spp.

  2. Identification and robust control of an experimental servo motor.

    PubMed

    Adam, E J; Guestrin, E D

    2002-04-01

    In this work, the design of a robust controller for an experimental laboratory-scale position control system based on a dc motor drive as well as the corresponding identification and robust stability analysis are presented. In order to carry out the robust design procedure, first, a classic closed-loop identification technique is applied and then, the parametrization by internal model control is used. The model uncertainty is evaluated under both parametric and global representation. For the latter case, an interesting discussion about the conservativeness of this description is presented by means of a comparison between the uncertainty disk and the critical perturbation radius approaches. Finally, conclusions about the performance of the experimental system with the robust controller are discussed using comparative graphics of the controlled variable and the Nyquist stability margin as a robustness measurement.

  3. CONTROL OF STREPTOCOCCAL THROAT INFECTIONS IN SCHOOLS—A Cooperative Program Followed in Orange County

    PubMed Central

    Russell, Edward Lee

    1956-01-01

    Attempts to identify streptococcal throat infections on clinical evidence alone do not provide an adequate or reliable index of the prevalence of these infections in the community. Epidemiologic information on streptococcal throat infections based on bacteriological identification permits a more accurate assessment of the situation and more logical and more effective control measures. Recent refinements in laboratory procedures have provided a simple, reliable and relatively inexpensive method for the identification of Group A beta hemolytic streptococci by public health or clinical laboratories. In Orange County a program for the identification of streptococcal throat infections by cooperative action of the medical profession, the health department and the school authorities greatly aided in control of the disease. A voluntary health agency (heart association) made an important contribution toward the success of the control program. PMID:13374555

  4. Nonlinear system identification technique validation

    NASA Astrophysics Data System (ADS)

    Rudko, M.; Bussgang, J. J.

    1982-01-01

    This final technical report describes the results obtained by SIGNATRON, Inc. of Lexington MA on Air Force Contract F30602-80-C-0104 for Rome Air Development Center. The objective of this effort is to develop a technique for identifying system response of nonlinear circuits by measurements of output response to known inputs. The report describes results of a study into the system identification technique based on the pencil-of-function method previously explored by Jain (1974) and Ewen (1979). The procedure identified roles of the linear response and is intended as a first step in nonlinear response and is intended as a first step in nonlinear circuit identification. There are serious implementation problems associated with the original approach such as loss of accuracy due to repeated integrations, lack of good measures of accuracy and computational iteration to identify the number of poles.

  5. Breast tumor segmentation in high resolution x-ray phase contrast analyzer based computed tomography.

    PubMed

    Brun, E; Grandl, S; Sztrókay-Gaul, A; Barbone, G; Mittone, A; Gasilov, S; Bravin, A; Coan, P

    2014-11-01

    Phase contrast computed tomography has emerged as an imaging method, which is able to outperform present day clinical mammography in breast tumor visualization while maintaining an equivalent average dose. To this day, no segmentation technique takes into account the specificity of the phase contrast signal. In this study, the authors propose a new mathematical framework for human-guided breast tumor segmentation. This method has been applied to high-resolution images of excised human organs, each of several gigabytes. The authors present a segmentation procedure based on the viscous watershed transform and demonstrate the efficacy of this method on analyzer based phase contrast images. The segmentation of tumors inside two full human breasts is then shown as an example of this procedure's possible applications. A correct and precise identification of the tumor boundaries was obtained and confirmed by manual contouring performed independently by four experienced radiologists. The authors demonstrate that applying the watershed viscous transform allows them to perform the segmentation of tumors in high-resolution x-ray analyzer based phase contrast breast computed tomography images. Combining the additional information provided by the segmentation procedure with the already high definition of morphological details and tissue boundaries offered by phase contrast imaging techniques, will represent a valuable multistep procedure to be used in future medical diagnostic applications.

  6. Development and validation of an automated operational modal analysis algorithm for vibration-based monitoring and tensile load estimation

    NASA Astrophysics Data System (ADS)

    Rainieri, Carlo; Fabbrocino, Giovanni

    2015-08-01

    In the last few decades large research efforts have been devoted to the development of methods for automated detection of damage and degradation phenomena at an early stage. Modal-based damage detection techniques are well-established methods, whose effectiveness for Level 1 (existence) and Level 2 (location) damage detection is demonstrated by several studies. The indirect estimation of tensile loads in cables and tie-rods is another attractive application of vibration measurements. It provides interesting opportunities for cheap and fast quality checks in the construction phase, as well as for safety evaluations and structural maintenance over the structure lifespan. However, the lack of automated modal identification and tracking procedures has been for long a relevant drawback to the extensive application of the above-mentioned techniques in the engineering practice. An increasing number of field applications of modal-based structural health and performance assessment are appearing after the development of several automated output-only modal identification procedures in the last few years. Nevertheless, additional efforts are still needed to enhance the robustness of automated modal identification algorithms, control the computational efforts and improve the reliability of modal parameter estimates (in particular, damping). This paper deals with an original algorithm for automated output-only modal parameter estimation. Particular emphasis is given to the extensive validation of the algorithm based on simulated and real datasets in view of continuous monitoring applications. The results point out that the algorithm is fairly robust and demonstrate its ability to provide accurate and precise estimates of the modal parameters, including damping ratios. As a result, it has been used to develop systems for vibration-based estimation of tensile loads in cables and tie-rods. Promising results have been achieved for non-destructive testing as well as continuous monitoring purposes. They are documented in the last sections of the paper.

  7. A National Coordinating Center for Prehospital Trauma Research Funding Transfusion Using Stored Fresh Whole Blood

    DTIC Science & Technology

    2016-09-01

    bank data base 28-30 5% Analyze blood bank data base 28-33 0% Other Major Tasks: Identification of communities in the UCLA catchment area 1-3 N/A...coagulopathy in real-time is underway. The Blood Bank is working to identify a pool of whole blood donors and incorporating the new product (FWB) in...Blood Bank , emergency Room, Trauma, Operating Room, Intensive Care Unit, etc) to coordinate and streamline standard operating procedures for the

  8. Transboundary EIA: Iberian experiences

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Albergaria, Rita; Fidelis, Teresa

    2006-10-15

    The 1314 km border shared by Portugal and Spain is simultaneously a conflict generator, due to joint access to common resources such as water, and a motive for transboundary cooperation in terms of development projects based on common concerns. Transboundary cooperation associated with Environment Impact Assessment (EIA) has been encouraged through the enactment of the Espoo Convention (1997). The European Union has adopted a Directive (97/11/CE Directive) under which taking transboundary impacts into consideration during EIA processes has become mandatory for member states. As a consequence, Portugal and Spain have approved related provisions. This paper aims to critically analyse themore » legal and procedural features of bilateral cooperation through the comparison of two case studies related to water management projects (the Sela and Alqueva dams). The paper highlights procedural weaknesses and puts forward a 'Good Practice Model' for cooperation under transboundary EIA processes. The model focuses on the ways in which EIA-based bilateral cooperation should proceed through the specification of phases and procedures for collaboration between Portugal and Spain in the identification and evaluation of transboundary impacts, as well in the public participation procedures.« less

  9. Rapid molecular identification and characteristics of Lactobacillus strains.

    PubMed

    Markiewicz, L H; Biedrzycka, E; Wasilewska, E; Bielecka, M

    2010-09-01

    Eleven type strains and 24 Lactobacillus isolates, preliminarily classified to the species due to phenotypic features, were investigated. Standard methods of identification with species-specific PCRs and typing with PFGE (with ApaI, NotI and SmaI restriction enzymes) allowed us to distinguish 16 unique strains belonging to 5 species (L. acidophilus, L. delbrueckii ssp. bulgaricus, L. plantarum, L. rhamnosus, L. salivarius). Alternative approach with 16S-23S rDNA ARDRA identification (with merely two restrictases, BsuRI and TaqI) and PCR-based typing (RAPD with two random- and rep-PCR with (GTG)(5) primers) showed to be more discriminative, i.e. 21 unique strains were classified in the same species as above. As a result, 7 out of 24 phenotypically species-assigned isolates were reclassified. The alternative procedure of rapid identification and typing of Lactobacillus isolates appeared to be equally effective and shortened from 1 week to 2-3 d (in comparison to the standard methods).

  10. Positive dental identification using tooth anatomy and digital superimposition.

    PubMed

    Johansen, Raymond J; Michael Bowers, C

    2013-03-01

    Dental identification of unknown human remains continues to be a relevant and reliable adjunct to forensic investigations. The advent of genomic and mitochondrial DNA procedures has not displaced the practical use of dental and related osseous structures remaining after destructive incidents that can render human remains unrecognizable, severely burned, and fragmented. The ability to conclusively identify victims of accident and homicide is based on the availability of antemortem records containing substantial and unambiguous proof of dental and related osseous characteristics. This case report documents the use of digital comparative analysis of antemortem dental models and postmortem dentition, to determine a dental identification. Images of dental models were digitally analyzed using Adobe Photoshop(TM) software. Individual tooth anatomy was compared between the antemortem and postmortem images. Digital superimposition techniques were also used for the comparison. With the absence of antemortem radiographs, this method proved useful to reach a positive identification in this case. © 2012 American Academy of Forensic Sciences.

  11. Modern control concepts in hydrology. [parameter identification in adaptive stochastic control approach

    NASA Technical Reports Server (NTRS)

    Duong, N.; Winn, C. B.; Johnson, G. R.

    1975-01-01

    Two approaches to an identification problem in hydrology are presented, based upon concepts from modern control and estimation theory. The first approach treats the identification of unknown parameters in a hydrologic system subject to noisy inputs as an adaptive linear stochastic control problem; the second approach alters the model equation to account for the random part in the inputs, and then uses a nonlinear estimation scheme to estimate the unknown parameters. Both approaches use state-space concepts. The identification schemes are sequential and adaptive and can handle either time-invariant or time-dependent parameters. They are used to identify parameters in the Prasad model of rainfall-runoff. The results obtained are encouraging and confirm the results from two previous studies; the first using numerical integration of the model equation along with a trial-and-error procedure, and the second using a quasi-linearization technique. The proposed approaches offer a systematic way of analyzing the rainfall-runoff process when the input data are imbedded in noise.

  12. Loop-Mediated Isothermal Amplification (LAMP): Emergence As an Alternative Technology for Herbal Medicine Identification.

    PubMed

    Li, Jing-Jian; Xiong, Chao; Liu, Yue; Liang, Jun-Song; Zhou, Xing-Wen

    2016-01-01

    Correct identification of medicinal plant ingredients is essential for their safe use and for the regulation of herbal drug supply chain. Loop-mediated isothermal amplification (LAMP) is a recently developed approach to identify herbal medicine species. This novel molecular biology technique enables timely and accurate testing, especially in settings where infrastructures to support polymerase chain reaction facilities are lacking. Studies that used this method have altered our view on the extent and complexity of herbal medicine identification. In this review, we give an introduction into LAMP analysis, covers the basic principles and important aspects in the development of LAMP analysis method. Then we presented a critical review of the application of LAMP-based methods in detecting and identifying raw medicinal plant materials and their processed products. We also provide a practical standard operating procedure (SOP) for the utilization of the LAMP protocol in herbal authentication, and consider the prospects of LAMP technology in the future developments of herbal medicine identification and the challenges associated with its application.

  13. Loop-Mediated Isothermal Amplification (LAMP): Emergence As an Alternative Technology for Herbal Medicine Identification

    PubMed Central

    Li, Jing-jian; Xiong, Chao; Liu, Yue; Liang, Jun-song; Zhou, Xing-wen

    2016-01-01

    Correct identification of medicinal plant ingredients is essential for their safe use and for the regulation of herbal drug supply chain. Loop-mediated isothermal amplification (LAMP) is a recently developed approach to identify herbal medicine species. This novel molecular biology technique enables timely and accurate testing, especially in settings where infrastructures to support polymerase chain reaction facilities are lacking. Studies that used this method have altered our view on the extent and complexity of herbal medicine identification. In this review, we give an introduction into LAMP analysis, covers the basic principles and important aspects in the development of LAMP analysis method. Then we presented a critical review of the application of LAMP-based methods in detecting and identifying raw medicinal plant materials and their processed products. We also provide a practical standard operating procedure (SOP) for the utilization of the LAMP protocol in herbal authentication, and consider the prospects of LAMP technology in the future developments of herbal medicine identification and the challenges associated with its application. PMID:28082999

  14. 49 CFR 1542.211 - Identification systems.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... secured area or SIDA continuously displays the identification medium issued to that individual on the... individual who has authorized unescorted access to secured areas and SIDA's to ascertain the authority of any... approved identification media. The procedure must— (1) Apply uniformly in secured areas, SIDAs, and...

  15. 49 CFR 1542.211 - Identification systems.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... secured area or SIDA continuously displays the identification medium issued to that individual on the... individual who has authorized unescorted access to secured areas and SIDA's to ascertain the authority of any... approved identification media. The procedure must— (1) Apply uniformly in secured areas, SIDAs, and...

  16. 49 CFR 1542.211 - Identification systems.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... secured area or SIDA continuously displays the identification medium issued to that individual on the... individual who has authorized unescorted access to secured areas and SIDA's to ascertain the authority of any... approved identification media. The procedure must— (1) Apply uniformly in secured areas, SIDAs, and...

  17. 49 CFR 1542.211 - Identification systems.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... secured area or SIDA continuously displays the identification medium issued to that individual on the... individual who has authorized unescorted access to secured areas and SIDA's to ascertain the authority of any... approved identification media. The procedure must— (1) Apply uniformly in secured areas, SIDAs, and...

  18. 49 CFR 1542.211 - Identification systems.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... secured area or SIDA continuously displays the identification medium issued to that individual on the... individual who has authorized unescorted access to secured areas and SIDA's to ascertain the authority of any... approved identification media. The procedure must— (1) Apply uniformly in secured areas, SIDAs, and...

  19. Predictors of eyewitness identification decisions from video lineups in England: a field study.

    PubMed

    Horry, Ruth; Memon, Amina; Wright, Daniel B; Milne, Rebecca

    2012-08-01

    Eyewitness identification decisions from 1,039 real lineups in England were analysed. Identification procedures have undergone dramatic change in the United Kingdom over recent years. Video lineups are now standard procedure, in which each lineup member is seen sequentially. The whole lineup is seen twice before the witness can make a decision, and the witness can request additional viewings of the lineup. A key aim of this paper was to investigate the association between repeated viewing and eyewitness decisions. Repeated viewing was strongly associated with increased filler identification rates, suggesting that witnesses who requested additional viewings were more willing to guess. In addition, several other factors were associated with lineup outcomes, including the age difference between the suspect and the witness, the type of crime committed, and delay. Overall, the suspect identification rate was 39%, the filler identification rate was 26% and the lineup rejection rate was 35%. (PsycINFO Database Record (c) 2012 APA, all rights reserved).

  20. Fuzzy model-based fault detection and diagnosis for a pilot heat exchanger

    NASA Astrophysics Data System (ADS)

    Habbi, Hacene; Kidouche, Madjid; Kinnaert, Michel; Zelmat, Mimoun

    2011-04-01

    This article addresses the design and real-time implementation of a fuzzy model-based fault detection and diagnosis (FDD) system for a pilot co-current heat exchanger. The design method is based on a three-step procedure which involves the identification of data-driven fuzzy rule-based models, the design of a fuzzy residual generator and the evaluation of the residuals for fault diagnosis using statistical tests. The fuzzy FDD mechanism has been implemented and validated on the real co-current heat exchanger, and has been proven to be efficient in detecting and isolating process, sensor and actuator faults.

  1. Techniques for the recovery and identification of Cryptosporidium oocysts from stool specimens.

    PubMed

    Garcia, L S; Bruckner, D A; Brewer, T C; Shimizu, R Y

    1983-07-01

    Due to increasing numbers of patients with documented infections with Cryptosporidium and other coccidia, it is important for the physician and clinical laboratory to be aware of the appropriate diagnostic techniques necessary for organism recovery and identification. Although Cryptosporidium is found in the gastrointestinal tract, tissue biopsies may be insufficient for organism recovery; the examination of stool specimens is a noninvasive procedure and will provide better overall opportunities for organism recovery. Human clinical specimens were examined from 45 patients with confirmed cryptosporidiosis or suspected of having the infection. Tissue biopsy sections, fecal wet preparations, and permanent stained smears were examined. Stool specimens were submitted in 10% Formalin, 2.5% potassium dichromate, and polyvinyl alcohol and were examined for oocysts by using 15 different methods: phase-contrast and light microscopy; Sheather's sugar flotation; Formalin concentration techniques; 10% potassium hydroxide; Giemsa; trichrome; periodic acid-Schiff; modified periodic acid-Schiff; silver methenamine; acridine orange; auramine-rhodamine; Kinyoun acid-fast; Ziehl-Neelsen carbolfuchsin; and a modified acid-fast procedure. Each technique or combination of techniques was assessed by organism quantitation, organism morphology, and ease of visual recognition. Based on these comparative studies, the modified Ziehl-Neelsen carbolfuchsin stain on 10% Formalin-preserved stool is recommended for the recovery and identification of Cryptosporidium.

  2. Techniques for the recovery and identification of Cryptosporidium oocysts from stool specimens.

    PubMed Central

    Garcia, L S; Bruckner, D A; Brewer, T C; Shimizu, R Y

    1983-01-01

    Due to increasing numbers of patients with documented infections with Cryptosporidium and other coccidia, it is important for the physician and clinical laboratory to be aware of the appropriate diagnostic techniques necessary for organism recovery and identification. Although Cryptosporidium is found in the gastrointestinal tract, tissue biopsies may be insufficient for organism recovery; the examination of stool specimens is a noninvasive procedure and will provide better overall opportunities for organism recovery. Human clinical specimens were examined from 45 patients with confirmed cryptosporidiosis or suspected of having the infection. Tissue biopsy sections, fecal wet preparations, and permanent stained smears were examined. Stool specimens were submitted in 10% Formalin, 2.5% potassium dichromate, and polyvinyl alcohol and were examined for oocysts by using 15 different methods: phase-contrast and light microscopy; Sheather's sugar flotation; Formalin concentration techniques; 10% potassium hydroxide; Giemsa; trichrome; periodic acid-Schiff; modified periodic acid-Schiff; silver methenamine; acridine orange; auramine-rhodamine; Kinyoun acid-fast; Ziehl-Neelsen carbolfuchsin; and a modified acid-fast procedure. Each technique or combination of techniques was assessed by organism quantitation, organism morphology, and ease of visual recognition. Based on these comparative studies, the modified Ziehl-Neelsen carbolfuchsin stain on 10% Formalin-preserved stool is recommended for the recovery and identification of Cryptosporidium. Images PMID:6193138

  3. Vector soup: high-throughput identification of Neotropical phlebotomine sand flies using metabarcoding.

    PubMed

    Kocher, Arthur; Gantier, Jean-Charles; Gaborit, Pascal; Zinger, Lucie; Holota, Helene; Valiere, Sophie; Dusfour, Isabelle; Girod, Romain; Bañuls, Anne-Laure; Murienne, Jerome

    2017-03-01

    Phlebotomine sand flies are haematophagous dipterans of primary medical importance. They represent the only proven vectors of leishmaniasis worldwide and are involved in the transmission of various other pathogens. Studying the ecology of sand flies is crucial to understand the epidemiology of leishmaniasis and further control this disease. A major limitation in this regard is that traditional morphological-based methods for sand fly species identifications are time-consuming and require taxonomic expertise. DNA metabarcoding holds great promise in overcoming this issue by allowing the identification of multiple species from a single bulk sample. Here, we assessed the reliability of a short insect metabarcode located in the mitochondrial 16S rRNA for the identification of Neotropical sand flies, and constructed a reference database for 40 species found in French Guiana. Then, we conducted a metabarcoding experiment on sand flies mixtures of known content and showed that the method allows an accurate identification of specimens in pools. Finally, we applied metabarcoding to field samples caught in a 1-ha forest plot in French Guiana. Besides providing reliable molecular data for species-level assignations of phlebotomine sand flies, our study proves the efficiency of metabarcoding based on the mitochondrial 16S rRNA for studying sand fly diversity from bulk samples. The application of this high-throughput identification procedure to field samples can provide great opportunities for vector monitoring and eco-epidemiological studies. © 2016 John Wiley & Sons Ltd.

  4. AgRISTARS: Foreign commodity production forecasting. Corn/soybean decision logic development and testing

    NASA Technical Reports Server (NTRS)

    Dailey, C. L.; Abotteen, K. M. (Principal Investigator)

    1980-01-01

    The development and testing of an analysis procedure which was developed to improve the consistency and objectively of crop identification using Landsat data is described. The procedure was developed to identify corn and soybean crops in the U.S. corn belt region. The procedure consists of a series of decision points arranged in a tree-like structure, the branches of which lead an analyst to crop labels. The specific decision logic is designed to maximize the objectively of the identification process and to promote the possibility of future automation. Significant results are summarized.

  5. Recursive inversion of externally defined linear systems by FIR filters

    NASA Technical Reports Server (NTRS)

    Bach, Ralph E., Jr.; Baram, Yoram

    1989-01-01

    The approximate inversion of an internally unknown linear system, given by its impulse response sequence, by an inverse system having a finite impulse response, is considered. The recursive least-squares procedure is shown to have an exact initialization, based on the triangular Toeplitz structure of the matrix involved. The proposed approach also suggests solutions to the problem of system identification and compensation.

  6. A Computational Procedure for Identifying Bilinear Representations of Nonlinear Systems Using Volterra Kernels

    NASA Technical Reports Server (NTRS)

    Kvaternik, Raymond G.; Silva, Walter A.

    2008-01-01

    A computational procedure for identifying the state-space matrices corresponding to discrete bilinear representations of nonlinear systems is presented. A key feature of the method is the use of first- and second-order Volterra kernels (first- and second-order pulse responses) to characterize the system. The present method is based on an extension of a continuous-time bilinear system identification procedure given in a 1971 paper by Bruni, di Pillo, and Koch. The analytical and computational considerations that underlie the original procedure and its extension to the title problem are presented and described, pertinent numerical considerations associated with the process are discussed, and results obtained from the application of the method to a variety of nonlinear problems from the literature are presented. The results of these exploratory numerical studies are decidedly promising and provide sufficient credibility for further examination of the applicability of the method.

  7. Implications of electronic health record downtime: an analysis of patient safety event reports.

    PubMed

    Larsen, Ethan; Fong, Allan; Wernz, Christian; Ratwani, Raj M

    2018-02-01

    We sought to understand the types of clinical processes, such as image and medication ordering, that are disrupted during electronic health record (EHR) downtime periods by analyzing the narratives of patient safety event report data. From a database of 80 381 event reports, 76 reports were identified as explicitly describing a safety event associated with an EHR downtime period. These reports were analyzed and categorized based on a developed code book to identify the clinical processes that were impacted by downtime. We also examined whether downtime procedures were in place and followed. The reports were coded into categories related to their reported clinical process: Laboratory, Medication, Imaging, Registration, Patient Handoff, Documentation, History Viewing, Delay of Procedure, and General. A majority of reports (48.7%, n = 37) were associated with lab orders and results, followed by medication ordering and administration (14.5%, n = 11). Incidents commonly involved patient identification and communication of clinical information. A majority of reports (46%, n = 35) indicated that downtime procedures either were not followed or were not in place. Only 27.6% of incidents (n = 21) indicated that downtime procedures were successfully executed. Patient safety report data offer a lens into EHR downtime-related safety hazards. Important areas of risk during EHR downtime periods were patient identification and communication of clinical information; these should be a focus of downtime procedure planning to reduce safety hazards. EHR downtime events pose patient safety hazards, and we highlight critical areas for downtime procedure improvement. © The Author 2017. Published by Oxford University Press on behalf of the American Medical Informatics Association. All rights reserved. For Permissions, please email: journals.permissions@oup.com

  8. Evaluation of molecular markers for Phytophthora ramorum detection and identification: testing for specificity using a standardized library of isolates.

    PubMed

    Martin, F N; Coffey, M D; Zeller, K; Hamelin, R C; Tooley, P; Garbelotto, M; Hughes, K J D; Kubisiak, T; Bilodeau, G J; Levy, L; Blomquist, C; Berger, P H

    2009-04-01

    Given the importance of Phytophthora ramorum from a regulatory standpoint, it is imperative that molecular markers for pathogen detection are fully tested to evaluate their specificity in detection of the pathogen. In an effort to evaluate 11 reported diagnostic techniques, we assembled a standardized DNA library using accessions from the World Phytophthora Genetic Resource Collection for 315 isolates representing 60 described Phytophthora spp. as well as 11 taxonomically unclassified isolates. These were sent blind to collaborators in seven laboratories to evaluate published diagnostic procedures using conventional (based on internal transcribed spacer [ITS] and cytochrome oxidase gene [cox]1 and 2 spacer regions) and real-time polymerase chain reaction (based on ITS and cox1 and 2 spacer regions as well as beta-tubulin and elicitin genes). Single-strand conformation polymorphism (SSCP) analysis using an automated sequencer for data collection was also evaluated for identification of all species tested. In general, the procedures worked well, with varying levels of specificity observed among the different techniques. With few exceptions, all assays correctly identified all isolates of P. ramorum and low levels of false positives were observed for the mitochondrial cox spacer markers and most of the real-time assays based on nuclear markers (diagnostic specificity between 96.9 and 100%). The highest level of false positives was obtained with the conventional nested ITS procedure; however, this technique is not stand-alone and is used in conjunction with two other assays for diagnostic purposes. The results indicated that using multiple assays improved the accuracy of the results compared with looking at a single assay alone, in particular when the markers represented different genetic loci. The SSCP procedure accurately identified P. ramorum and was helpful in classification of a number of isolates to a species level. With one exception, all procedures accurately identified P. ramorum in blind evaluations of 60 field samples that included examples of plant infection by 11 other Phytophthora spp. The SSCP analysis identified eight of these species, with three identified to a species group.

  9. 30 CFR 281.14 - OCS mining area identification.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 30 Mineral Resources 2 2011-07-01 2011-07-01 false OCS mining area identification. 281.14 Section 281.14 Mineral Resources BUREAU OF OCEAN ENERGY MANAGEMENT, REGULATION, AND ENFORCEMENT, DEPARTMENT OF... SHELF Leasing Procedures § 281.14 OCS mining area identification. The Secretary, after considering the...

  10. 24 CFR 5.216 - Disclosure and verification of Social Security and Employer Identification Numbers.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... Social Security and Employer Identification Numbers. 5.216 Section 5.216 Housing and Urban Development...; WAIVERS Disclosure and Verification of Social Security Numbers and Employer Identification Numbers; Procedures for Obtaining Income Information Disclosure and Verification of Social Security Numbers and...

  11. 47 CFR 80.104 - Identification of radar transmissions not authorized.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 47 Telecommunication 5 2011-10-01 2011-10-01 false Identification of radar transmissions not... Procedures-General § 80.104 Identification of radar transmissions not authorized. This section applies to all maritime radar transmitters except radar beacon stations. (a) Radar transmitters must not transmit station...

  12. 47 CFR 80.104 - Identification of radar transmissions not authorized.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 47 Telecommunication 5 2010-10-01 2010-10-01 false Identification of radar transmissions not... Procedures-General § 80.104 Identification of radar transmissions not authorized. This section applies to all maritime radar transmitters except radar beacon stations. (a) Radar transmitters must not transmit station...

  13. 24 CFR 5.216 - Disclosure and verification of Social Security and Employer Identification Numbers.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... Social Security and Employer Identification Numbers. 5.216 Section 5.216 Housing and Urban Development...; WAIVERS Disclosure and Verification of Social Security Numbers and Employer Identification Numbers; Procedures for Obtaining Income Information Disclosure and Verification of Social Security Numbers and...

  14. 24 CFR 5.216 - Disclosure and verification of Social Security and Employer Identification Numbers.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... Social Security and Employer Identification Numbers. 5.216 Section 5.216 Housing and Urban Development...; WAIVERS Disclosure and Verification of Social Security Numbers and Employer Identification Numbers; Procedures for Obtaining Income Information Disclosure and Verification of Social Security Numbers and...

  15. 24 CFR 5.216 - Disclosure and verification of Social Security and Employer Identification Numbers.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... Social Security and Employer Identification Numbers. 5.216 Section 5.216 Housing and Urban Development...; WAIVERS Disclosure and Verification of Social Security Numbers and Employer Identification Numbers; Procedures for Obtaining Income Information Disclosure and Verification of Social Security Numbers and...

  16. 47 CFR 80.104 - Identification of radar transmissions not authorized.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 47 Telecommunication 5 2012-10-01 2012-10-01 false Identification of radar transmissions not... Procedures-General § 80.104 Identification of radar transmissions not authorized. This section applies to all maritime radar transmitters except radar beacon stations. (a) Radar transmitters must not transmit station...

  17. 47 CFR 80.104 - Identification of radar transmissions not authorized.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 47 Telecommunication 5 2014-10-01 2014-10-01 false Identification of radar transmissions not... Procedures-General § 80.104 Identification of radar transmissions not authorized. This section applies to all maritime radar transmitters except radar beacon stations. (a) Radar transmitters must not transmit station...

  18. 47 CFR 80.104 - Identification of radar transmissions not authorized.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 47 Telecommunication 5 2013-10-01 2013-10-01 false Identification of radar transmissions not... Procedures-General § 80.104 Identification of radar transmissions not authorized. This section applies to all maritime radar transmitters except radar beacon stations. (a) Radar transmitters must not transmit station...

  19. 24 CFR 5.216 - Disclosure and verification of Social Security and Employer Identification Numbers.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... Social Security and Employer Identification Numbers. 5.216 Section 5.216 Housing and Urban Development...; WAIVERS Disclosure and Verification of Social Security Numbers and Employer Identification Numbers; Procedures for Obtaining Income Information Disclosure and Verification of Social Security Numbers and...

  20. Analysis of pilot control strategy

    NASA Technical Reports Server (NTRS)

    Heffley, R. K.; Hanson, G. D.; Jewell, W. F.; Clement, W. F.

    1983-01-01

    Methods for nonintrusive identification of pilot control strategy and task execution dynamics are presented along with examples based on flight data. The specific analysis technique is Nonintrusive Parameter Identification Procedure (NIPIP), which is described in a companion user's guide (NASA CR-170398). Quantification of pilot control strategy and task execution dynamics is discussed in general terms followed by a more detailed description of how NIPIP can be applied. The examples are based on flight data obtained from the NASA F-8 digital fly by wire airplane. These examples involve various piloting tasks and control axes as well as a demonstration of how the dynamics of the aircraft itself are identified using NIPIP. Application of NIPIP to the AFTI/F-16 flight test program is discussed. Recommendations are made for flight test applications in general and refinement of NIPIP to include interactive computer graphics.

  1. A simplified chair-side remount technique using customized mounting platforms.

    PubMed

    Chauhan, Mamta Devendrakumar; Dange, Shankar Pandharinath; Khalikar, Arun Narayan; Vaidya, Smita Padmakar

    2012-08-01

    Correct occlusal relationships are part of the successful prosthetic treatment for edentulous patients. Fabrication of complete dentures comprises of clinical and laboratory procedures that should be executed accurately for achieving success with fabricated dentures. Errors occurring during the clinical and laboratory procedures of a denture may subsequently lead to the occlusal errors in the final prosthesis. These occlusal errors can be corrected in two ways: i) in patient's mouth ii) by recording new centric relation and remounting dentures on an articulator. The latter method is more feasible because the mobility of denture base on the mucosa in oral cavity does not permit the identification of premature contacts in centric occlusion and tooth guided eccentric excursions. This article describes a modest and effective clinical chair-side remount procedure using customized mounting platforms.

  2. A simplified chair-side remount technique using customized mounting platforms

    PubMed Central

    Dange, Shankar Pandharinath; Khalikar, Arun Narayan; Vaidya, Smita Padmakar

    2012-01-01

    Correct occlusal relationships are part of the successful prosthetic treatment for edentulous patients. Fabrication of complete dentures comprises of clinical and laboratory procedures that should be executed accurately for achieving success with fabricated dentures. Errors occurring during the clinical and laboratory procedures of a denture may subsequently lead to the occlusal errors in the final prosthesis. These occlusal errors can be corrected in two ways: i) in patient's mouth ii) by recording new centric relation and remounting dentures on an articulator. The latter method is more feasible because the mobility of denture base on the mucosa in oral cavity does not permit the identification of premature contacts in centric occlusion and tooth guided eccentric excursions. This article describes a modest and effective clinical chair-side remount procedure using customized mounting platforms. PMID:22977726

  3. Mapping of Rill Erosion of Arable Soils Based on Unmanned Aerial Vehicles Survey

    NASA Astrophysics Data System (ADS)

    Kashtanov, A. N.; Vernyuk, Yu. I.; Savin, I. Yu.; Shchepot'ev, V. V.; Dokukin, P. A.; Sharychev, D. V.; Li, K. A.

    2018-04-01

    Possibilities of using data obtained from unmanned aerial vehicles for detection and mapping of rill erosion on arable lands are analyzed. Identification and mapping of rill erosion was performed on a key plot with a predominance of arable gray forest soils (Greyzemic Phaeozems) under winter wheat in Tula oblast. This plot was surveyed from different heights and in different periods to determine the reliability of identification of rill erosion on the basis of automated procedures in a GIS. It was found that, despite changes in the pattern of rills during the warm season, only one survey during this season is sufficient for adequate assessment of the area of eroded soils. According to our data, the most reliable identification of rill erosion is based on the aerial survey from the height of 50 m above the soil surface. When the height of the flight is more than 200 m, erosional rills virtually escape identification. The efficiency of identification depends on the type of crops, their status, and time of the survey. The surveys of bare soil surface in periods with maximum possible interval from the previous rain or snowmelt season are most efficient. The results of our study can be used in the systems of remote sensing monitoring of erosional processes on arable fields. Application of multiand hyperspectral cameras can improve the efficiency of monitoring.

  4. 21 CFR 58.81 - Standard operating procedures.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... LABORATORY PRACTICE FOR NONCLINICAL LABORATORY STUDIES Testing Facilities Operation § 58.81 Standard operating procedures. (a) A testing facility shall have standard operating procedures in writing setting... following: (1) Animal room preparation. (2) Animal care. (3) Receipt, identification, storage, handling...

  5. 21 CFR 58.81 - Standard operating procedures.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... LABORATORY PRACTICE FOR NONCLINICAL LABORATORY STUDIES Testing Facilities Operation § 58.81 Standard operating procedures. (a) A testing facility shall have standard operating procedures in writing setting... following: (1) Animal room preparation. (2) Animal care. (3) Receipt, identification, storage, handling...

  6. 21 CFR 58.81 - Standard operating procedures.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... LABORATORY PRACTICE FOR NONCLINICAL LABORATORY STUDIES Testing Facilities Operation § 58.81 Standard operating procedures. (a) A testing facility shall have standard operating procedures in writing setting... following: (1) Animal room preparation. (2) Animal care. (3) Receipt, identification, storage, handling...

  7. 21 CFR 58.81 - Standard operating procedures.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... LABORATORY PRACTICE FOR NONCLINICAL LABORATORY STUDIES Testing Facilities Operation § 58.81 Standard operating procedures. (a) A testing facility shall have standard operating procedures in writing setting... following: (1) Animal room preparation. (2) Animal care. (3) Receipt, identification, storage, handling...

  8. Qualitative Determination of Nitrate with Triphenylbenzylphosphonium Chloride.

    ERIC Educational Resources Information Center

    Berry, Donna A.; Cole, Jerry J.

    1984-01-01

    Discusses two procedures for the identification of nitrate, the standard test ("Brown Ring" test) and a new procedure using triphenylbenzylphosphonium chloride (TPBPC). Effectiveness of both procedures is compared, with the TPBPC test proving to be more sensitive and accurate. (JM)

  9. Post-procedural Care in Interventional Radiology: What Every Interventional Radiologist Should Know—Part I: Standard Post-procedural Instructions and Follow-Up Care

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Taslakian, Bedros, E-mail: Bedros.Taslakian@nyumc.org; Sridhar, Divya

    Interventional radiology (IR) has evolved into a full-fledged clinical specialty with attendant patient care responsibilities. Success in IR now requires development of a full clinical practice, including consultations, inpatient admitting privileges, and an outpatient clinic. In addition to technical excellence and innovation, maintaining a comprehensive practice is imperative for interventional radiologists to compete successfully for patients and referral bases. A structured approach to periprocedural care, including routine follow-up and early identification and management of complications, facilitates efficient and thorough management with an emphasis on quality and patient safety.

  10. Identification and preparation of sperm for ART.

    PubMed

    Mehta, Akanksha; Sigman, Mark

    2014-02-01

    State-of-the-art techniques attempt to select sperm with the best functional capacity to produce pregnancy and, subsequently, healthy offspring. A variety of approaches are now being evaluated. Future approaches may allow for selection of sperm based on sperm DNA integrity, degree of aneuploidy, or apoptosis. Other approaches involve attempting to improve the in vitro function of sperm with exposure to compounds such as pentoxifylline or platelet activating factor. In the future, we are likely to see significant improvements in the ability to select the best sperm for assisted-reproductive-technology procedures and the use of these procedures in routine clinical practice. Copyright © 2014 Elsevier Inc. All rights reserved.

  11. Post-procedural Care in Interventional Radiology: What Every Interventional Radiologist Should Know-Part I: Standard Post-procedural Instructions and Follow-Up Care.

    PubMed

    Taslakian, Bedros; Sridhar, Divya

    2017-04-01

    Interventional radiology (IR) has evolved into a full-fledged clinical specialty with attendant patient care responsibilities. Success in IR now requires development of a full clinical practice, including consultations, inpatient admitting privileges, and an outpatient clinic. In addition to technical excellence and innovation, maintaining a comprehensive practice is imperative for interventional radiologists to compete successfully for patients and referral bases. A structured approach to periprocedural care, including routine follow-up and early identification and management of complications, facilitates efficient and thorough management with an emphasis on quality and patient safety.

  12. Quality Management and Key Performance Indicators in Oncologic Esophageal Surgery.

    PubMed

    Gockel, Ines; Ahlbrand, Constantin Johannes; Arras, Michael; Schreiber, Elke Maria; Lang, Hauke

    2015-12-01

    Ranking systems and comparisons of quality and performance indicators will be of increasing relevance for complex "high-risk" procedures such as esophageal cancer surgery. The identification of evidence-based standards relevant for key performance indicators in esophageal surgery is essential for establishing monitoring systems and furthermore a requirement to enhance treatment quality. In the course of this review, we analyze the key performance indicators case volume, radicality of resection, and postoperative morbidity and mortality, leading to continuous quality improvement. Ranking systems established on this basis will gain increased relevance in highly complex procedures within the national and international comparison and furthermore improve the treatment of patients with esophageal carcinoma.

  13. Rapid identification of illegal synthetic adulterants in herbal anti-diabetic medicines using near infrared spectroscopy

    NASA Astrophysics Data System (ADS)

    Feng, Yanchun; Lei, Deqing; Hu, Changqin

    We created a rapid detection procedure for identifying herbal medicines illegally adulterated with synthetic drugs using near infrared spectroscopy. This procedure includes a reverse correlation coefficient method (RCCM) and comparison of characteristic peaks. Moreover, we made improvements to the RCCM based on new strategies for threshold settings. Any tested herbal medicine must meet two criteria to be identified with our procedure as adulterated. First, the correlation coefficient between the tested sample and the reference must be greater than the RCCM threshold. Next, the NIR spectrum of the tested sample must contain the same characteristic peaks as the reference. In this study, four pure synthetic anti-diabetic drugs (i.e., metformin, gliclazide, glibenclamide and glimepiride), 174 batches of laboratory samples and 127 batches of herbal anti-diabetic medicines were used to construct and validate the procedure. The accuracy of this procedure was greater than 80%. Our data suggest that this protocol is a rapid screening tool to identify synthetic drug adulterants in herbal medicines on the market.

  14. An automatic identification procedure to promote the use of FES-cycling training for hemiparetic patients.

    PubMed

    Ambrosini, Emilia; Ferrante, Simona; Schauer, Thomas; Ferrigno, Giancarlo; Molteni, Franco; Pedrocchi, Alessandra

    2014-01-01

    Cycling induced by Functional Electrical Stimulation (FES) training currently requires a manual setting of different parameters, which is a time-consuming and scarcely repeatable procedure. We proposed an automatic procedure for setting session-specific parameters optimized for hemiparetic patients. This procedure consisted of the identification of the stimulation strategy as the angular ranges during which FES drove the motion, the comparison between the identified strategy and the physiological muscular activation strategy, and the setting of the pulse amplitude and duration of each stimulated muscle. Preliminary trials on 10 healthy volunteers helped define the procedure. Feasibility tests on 8 hemiparetic patients (5 stroke, 3 traumatic brain injury) were performed. The procedure maximized the motor output within the tolerance constraint, identified a biomimetic strategy in 6 patients, and always lasted less than 5 minutes. Its reasonable duration and automatic nature make the procedure usable at the beginning of every training session, potentially enhancing the performance of FES-cycling training.

  15. Results from the Crop Identification Technology Assessment for Remote Sensing (CITARS) project

    NASA Technical Reports Server (NTRS)

    Bauer, M. E. (Principal Investigator); Davis, B. J.; Bizzell, R. M.; Hall, F. G.; Feiveson, A. H.; Malila, W. A.; Rice, D. P.

    1976-01-01

    The author has identified the following significant results. It was found that several factors had a significant effect on crop identification performance: (1) crop maturity and site characteristics, (2) which of several different single date automatic data processing procedures was used for local recognition, (3) nonlocal recognition, both with and without preprocessing for the extension of recognition signatures, and (4) use of multidate data. It also was found that classification accuracy for field center pixels was not a reliable indicator of proportion estimation performance for whole areas, that bias was present in proportion estimates, and that training data and procedures strongly influenced crop identification performance.

  16. 24 CFR 5.238 - Criminal and civil penalties.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... Development GENERAL HUD PROGRAM REQUIREMENTS; WAIVERS Disclosure and Verification of Social Security Numbers and Employer Identification Numbers; Procedures for Obtaining Income Information Procedures for...

  17. Lattice Symmetry and Identification-The Fundamental Role of Reduced Cells in Materials Characterization.

    PubMed

    Mighell, A D

    2001-01-01

    In theory, physical crystals can be represented by idealized mathematical lattices. Under appropriate conditions, these representations can be used for a variety of purposes such as identifying, classifying, and understanding the physical properties of materials. Critical to these applications is the ability to construct a unique representation of the lattice. The vital link that enabled this theory to be realized in practice was provided by the 1970 paper on the determination of reduced cells. This seminal paper led to a mathematical approach to lattice analysis initially based on systematic reduction procedures and the use of standard cells. Subsequently, the process evolved to a matrix approach based on group theory and linear algebra that offered a more abstract and powerful way to look at lattices and their properties. Application of the reduced cell to both database work and laboratory research at NIST was immediately successful. Currently, this cell and/or procedures based on reduction are widely and routinely used by the general scientific community: (i) for calculating standard cells for the reporting of crystalline materials, (ii) for classifying materials, (iii) in crystallographic database work (iv) in routine x-ray and neutron diffractometry, and (v) in general crystallographic research. Especially important is its use in symmetry determination and in identification. The focus herein is on the role of the reduced cell in lattice symmetry determination.

  18. An automatic method to detect and track the glottal gap from high speed videoendoscopic images.

    PubMed

    Andrade-Miranda, Gustavo; Godino-Llorente, Juan I; Moro-Velázquez, Laureano; Gómez-García, Jorge Andrés

    2015-10-29

    The image-based analysis of the vocal folds vibration plays an important role in the diagnosis of voice disorders. The analysis is based not only on the direct observation of the video sequences, but also in an objective characterization of the phonation process by means of features extracted from the recorded images. However, such analysis is based on a previous accurate identification of the glottal gap, which is the most challenging step for a further automatic assessment of the vocal folds vibration. In this work, a complete framework to automatically segment and track the glottal area (or glottal gap) is proposed. The algorithm identifies a region of interest that is adapted along time, and combine active contours and watershed transform for the final delineation of the glottis and also an automatic procedure for synthesize different videokymograms is proposed. Thanks to the ROI implementation, our technique is robust to the camera shifting and also the objective test proved the effectiveness and performance of the approach in the most challenging scenarios that it is when exist an inappropriate closure of the vocal folds. The novelties of the proposed algorithm relies on the used of temporal information for identify an adaptive ROI and the use of watershed merging combined with active contours for the glottis delimitation. Additionally, an automatic procedure for synthesize multiline VKG by the identification of the glottal main axis is developed.

  19. 41 CFR 109-27.5011 - Identification marking of metals and metal products.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 41 Public Contracts and Property Management 3 2010-07-01 2010-07-01 false Identification marking of metals and metal products. 109-27.5011 Section 109-27.5011 Public Contracts and Property..., Procedures, and Guidelines § 109-27.5011 Identification marking of metals and metal products. ...

  20. 41 CFR 109-27.5011 - Identification marking of metals and metal products.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 41 Public Contracts and Property Management 3 2011-01-01 2011-01-01 false Identification marking of metals and metal products. 109-27.5011 Section 109-27.5011 Public Contracts and Property..., Procedures, and Guidelines § 109-27.5011 Identification marking of metals and metal products. ...

  1. Instruments Used in the Identification of Gifted and Talented Students.

    ERIC Educational Resources Information Center

    Callahan, Carolyn M.; Hunsaker, Scott L.; Adams, Cheryll M.; Moore, Sara D.; Bland, Lori C.

    This report presents findings of a study of instruments used in the identification of gifted and talented students. The study first examined the published literature, both standardized and locally developed identification instruments and procedures, and strategies used to identify underserved populations. These data were catalogued in the National…

  2. 9 CFR 147.12 - Procedures for collection, isolation, and identification of Salmonella from environmental samples...

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ..., and identification of Salmonella from environmental samples, cloacal swabs, chick box papers, and... identification of Salmonella from environmental samples, cloacal swabs, chick box papers, and meconium samples... chickens, waterfowl, exhibition poultry, and game birds. All samples and swabs described in this paragraph...

  3. 9 CFR 147.12 - Procedures for collection, isolation, and identification of Salmonella from environmental samples...

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ..., and identification of Salmonella from environmental samples, cloacal swabs, chick box papers, and... identification of Salmonella from environmental samples, cloacal swabs, chick box papers, and meconium samples... chickens, waterfowl, exhibition poultry, and game birds. All samples and swabs described in this paragraph...

  4. 9 CFR 147.12 - Procedures for collection, isolation, and identification of Salmonella from environmental samples...

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ..., and identification of Salmonella from environmental samples, cloacal swabs, chick box papers, and... identification of Salmonella from environmental samples, cloacal swabs, chick box papers, and meconium samples... chickens, waterfowl, exhibition poultry, and game birds. All samples and swabs described in this paragraph...

  5. 9 CFR 147.12 - Procedures for collection, isolation, and identification of Salmonella from environmental samples...

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ..., and identification of Salmonella from environmental samples, cloacal swabs, chick box papers, and... identification of Salmonella from environmental samples, cloacal swabs, chick box papers, and meconium samples... chickens, waterfowl, exhibition poultry, and game birds. All samples and swabs described in this paragraph...

  6. 9 CFR 147.12 - Procedures for collection, isolation, and identification of Salmonella from environmental samples...

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ..., and identification of Salmonella from environmental samples, cloacal swabs, chick box papers, and... identification of Salmonella from environmental samples, cloacal swabs, chick box papers, and meconium samples... chickens, waterfowl, exhibition poultry, and game birds. All samples and swabs described in this paragraph...

  7. The University of Michigan Campus Identification Signs.

    ERIC Educational Resources Information Center

    Michigan Univ., Ann Arbor.

    Specific information, identification, and direction to and within the campus as factors determining a campus sign system are discussed in terms of--(1) needs requiring an identification sign system, (2) recommendations for initiating a comprehensive sign system for the university, and (3) application procedures as they relate to streets, walkways,…

  8. 19 CFR 24.5 - Filing identification number.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 19 Customs Duties 1 2010-04-01 2010-04-01 false Filing identification number. 24.5 Section 24.5... TREASURY CUSTOMS FINANCIAL AND ACCOUNTING PROCEDURE § 24.5 Filing identification number. (a) Generally..., Notification of Importer's Number or Application for Importer's Number, or Notice of Change of Name or Address...

  9. 19 CFR 24.5 - Filing identification number.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... 19 Customs Duties 1 2013-04-01 2013-04-01 false Filing identification number. 24.5 Section 24.5... TREASURY CUSTOMS FINANCIAL AND ACCOUNTING PROCEDURE § 24.5 Filing identification number. (a) Generally..., Notification of Importer's Number or Application for Importer's Number, or Notice of Change of Name or Address...

  10. 19 CFR 24.5 - Filing identification number.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 19 Customs Duties 1 2011-04-01 2011-04-01 false Filing identification number. 24.5 Section 24.5... TREASURY CUSTOMS FINANCIAL AND ACCOUNTING PROCEDURE § 24.5 Filing identification number. (a) Generally..., Notification of Importer's Number or Application for Importer's Number, or Notice of Change of Name or Address...

  11. 48 CFR 243.170 - Identification of foreign military sale (FMS) requirements.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 48 Federal Acquisition Regulations System 3 2010-10-01 2010-10-01 false Identification of foreign military sale (FMS) requirements. 243.170 Section 243.170 Federal Acquisition Regulations System DEFENSE....170 Identification of foreign military sale (FMS) requirements. Follow the procedures at PGI 243.170...

  12. Molecular toolbox for the identification of unknown genetically modified organisms.

    PubMed

    Ruttink, Tom; Demeyer, Rolinde; Van Gulck, Elke; Van Droogenbroeck, Bart; Querci, Maddalena; Taverniers, Isabel; De Loose, Marc

    2010-03-01

    Competent laboratories monitor genetically modified organisms (GMOs) and products derived thereof in the food and feed chain in the framework of labeling and traceability legislation. In addition, screening is performed to detect the unauthorized presence of GMOs including asynchronously authorized GMOs or GMOs that are not officially registered for commercialization (unknown GMOs). Currently, unauthorized or unknown events are detected by screening blind samples for commonly used transgenic elements, such as p35S or t-nos. If (1) positive detection of such screening elements shows the presence of transgenic material and (2) all known GMOs are tested by event-specific methods but are not detected, then the presence of an unknown GMO is inferred. However, such evidence is indirect because it is based on negative observations and inconclusive because the procedure does not identify the causative event per se. In addition, detection of unknown events is hampered in products that also contain known authorized events. Here, we outline alternative approaches for analytical detection and GMO identification and develop new methods to complement the existing routine screening procedure. We developed a fluorescent anchor-polymerase chain reaction (PCR) method for the identification of the sequences flanking the p35S and t-nos screening elements. Thus, anchor-PCR fingerprinting allows the detection of unique discriminative signals per event. In addition, we established a collection of in silico calculated fingerprints of known events to support interpretation of experimentally generated anchor-PCR GM fingerprints of blind samples. Here, we first describe the molecular characterization of a novel GMO, which expresses recombinant human intrinsic factor in Arabidopsis thaliana. Next, we purposefully treated the novel GMO as a blind sample to simulate how the new methods lead to the molecular identification of a novel unknown event without prior knowledge of its transgene sequence. The results demonstrate that the new methods complement routine screening procedures by providing direct conclusive evidence and may also be useful to resolve masking of unknown events by known events.

  13. ICT and mobile health to improve clinical process delivery. a research project for therapy management process innovation.

    PubMed

    Locatelli, Paolo; Montefusco, Vittorio; Sini, Elena; Restifo, Nicola; Facchini, Roberta; Torresani, Michele

    2013-01-01

    The volume and the complexity of clinical and administrative information make Information and Communication Technologies (ICTs) essential for running and innovating healthcare. This paper tells about a project aimed to design, develop and implement a set of organizational models, acknowledged procedures and ICT tools (Mobile & Wireless solutions and Automatic Identification and Data Capture technologies) to improve actual support, safety, reliability and traceability of a specific therapy management (stem cells). The value of the project is to design a solution based on mobile and identification technology in tight collaboration with physicians and actors involved in the process to ensure usability and effectivenes in process management.

  14. Identification of input variables for feature based artificial neural networks-saccade detection in EOG recordings.

    PubMed

    Tigges, P; Kathmann, N; Engel, R R

    1997-07-01

    Though artificial neural networks (ANN) are excellent tools for pattern recognition problems when signal to noise ratio is low, the identification of decision relevant features for ANN input data is still a crucial issue. The experience of the ANN designer and the existing knowledge and understanding of the problem seem to be the only links for a specific construction. In the present study a backpropagation ANN based on modified raw data inputs showed encouraging results. Investigating the specific influences of prototypical input patterns on a specially designed ANN led to a new sparse and efficient input data presentation. This data coding obtained by a semiautomatic procedure combining existing expert knowledge and the internal representation structures of the raw data based ANN yielded a list of feature vectors, each representing the relevant information for saccade identification. The feature based ANN produced a reduction of the error rate of nearly 40% compared with the raw data ANN. An overall correct classification of 92% of so far unknown data was realized. The proposed method of extracting internal ANN knowledge for the production of a better input data representation is not restricted to EOG recordings, and could be used in various fields of signal analysis.

  15. A combined qualitative-quantitative approach for the identification of highly co-creative technology-driven firms

    NASA Astrophysics Data System (ADS)

    Milyakov, Hristo; Tanev, Stoyan; Ruskov, Petko

    2011-03-01

    Value co-creation, is an emerging business and innovation paradigm, however, there is not enough clarity on the distinctive characteristics of value co-creation as compared to more traditional value creation approaches. The present paper summarizes the results from an empirically-derived research study focusing on the development of a systematic procedure for the identification of firms that are active in value co-creation. The study is based on a sample 273 firms that were selected for being representative of the breadth of their value co-creation activities. The results include: i) the identification of the key components of value co-creation based on a research methodology using web search and Principal Component Analysis techniques, and ii) the comparison of two different classification techniques identifying the firms with the highest degree of involvement in value co-creation practices. To the best of our knowledge this is the first study using sophisticated data collection techniques to provide a classification of firms according to the degree of their involvement in value co-creation.

  16. Screening tests for pathogenic corynebacteria.

    PubMed Central

    Colman, G; Weaver, E; Efstratiou, A

    1992-01-01

    AIM: To provide simple tests that would help in the identification of corynebacteria that produce diphtheria toxin. METHODS: A collection of 99 freshly isolated corynebacteria was assembled and the cultures identified by conventional tests confirmed by an identification kit. Modifications were made to procedures for preparation of the culture medium for the Elek test and to the test for detection of pyrazinamidase (pyrazine carboxylamidase) activity. These two together with an indicator medium for cystinase activity were applied to the collection of organisms. RESULTS: Cystinase was detected in all 61 members of the toxigenic species and none produced pyrazinamidase. In contrast, all but two of the 38 representatives of non-toxigenic species yielded pyrazinamidase and none formed cystinase. Of the 61 cystinase producing cultures (which were also pyrazinamidase negative), 21 gave a positive Elek test with the modified culture medium. A total of 30 of these 61 were tested for toxigenicity in guinea pigs and the results of the animal and plate tests concorded. At least seven cultures could have been reported as non-toxigenic if Elek tests based on media prepared in the conventional way had been the only test available. CONCLUSION: The three procedures described go some way towards meeting the needs of diagnostic laboratories for efficient procedures for distinguishing pathogenic corynebacteria. PMID:1740514

  17. Damage identification on spatial Timoshenko arches by means of genetic algorithms

    NASA Astrophysics Data System (ADS)

    Greco, A.; D'Urso, D.; Cannizzaro, F.; Pluchino, A.

    2018-05-01

    In this paper a procedure for the dynamic identification of damage in spatial Timoshenko arches is presented. The proposed approach is based on the calculation of an arbitrary number of exact eigen-properties of a damaged spatial arch by means of the Wittrick and Williams algorithm. The proposed damage model considers a reduction of the volume in a part of the arch, and is therefore suitable, differently than what is commonly proposed in the main part of the dedicated literature, not only for concentrated cracks but also for diffused damaged zones which may involve a loss of mass. Different damage scenarios can be taken into account with variable location, intensity and extension of the damage as well as number of damaged segments. An optimization procedure, aiming at identifying which damage configuration minimizes the difference between its eigen-properties and a set of measured modal quantities for the structure, is implemented making use of genetic algorithms. In this context, an initial random population of chromosomes, representing different damage distributions along the arch, is forced to evolve towards the fittest solution. Several applications with different, single or multiple, damaged zones and boundary conditions confirm the validity and the applicability of the proposed procedure even in presence of instrumental errors on the measured data.

  18. A novel multiplex PCR for the simultaneous detection of Salmonella enterica and Shigella species.

    PubMed

    Radhika, M; Saugata, Majumder; Murali, H S; Batra, H V

    2014-01-01

    Salmonella enterica and Shigella species are commonly associated with food and water borne infections leading to gastrointestinal diseases. The present work was undertaken to develop a sensitive and reliable PCR based detection system for simultaneous detection of Salmonella enterica and Shigella at species level. For this the conserved regions of specific genes namely ipaH1, ipaH, wbgZ, wzy and invA were targeted for detection of Shigella genus, S. flexneri, S. sonnei, S. boydii and Salmonella enterica respectively along with an internal amplification control (IAC). The results showed that twenty Salmonella and eleven Shigella spp., were accurately identified by the assay without showing non-specificity against closely related other Enterobacteriaceae organisms and also against other pathogens. Further evaluation of multiplex PCR was undertaken on 50 natural samples of chicken, eggs and poultry litter and results compared with conventional culture isolation and identification procedure. The multiplex PCR identified the presence of Salmonella and Shigella strains with a short pre-enrichment step of 5 h in peptone water and the same samples were processed by conventional procedures for comparison. Therefore, this reported multiplex PCR can serve as an alternative to the tedious time-consuming procedure of culture and identification in food safety laboratories.

  19. 40 CFR 255.23 - Joint identification of agencies.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... solid waste and water quality management planning agencies, and all areawide agencies and the state... IDENTIFICATION OF REGIONS AND AGENCIES FOR SOLID WASTE MANAGEMENT Procedures for Identifying Regions and Agencies...

  20. 24 CFR 5.212 - Compliance with the Privacy Act and other requirements.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... Verification of Social Security Numbers and Employer Identification Numbers; Procedures for Obtaining Income... SSNs and Employer Identification Numbers (EINs), and income information under this subpart shall be...

  1. Exploration of Terminal Procedures Enabled by NASA Wake VAS Technologies

    NASA Technical Reports Server (NTRS)

    Lunsford, Clark R.; Smith, Arthur P., III; Cooper, Wayne W., Jr.; Mundra, Anand D.; Gross, Amy E.; Audenaerd, Laurence F.; Killian, Bruce E.

    2004-01-01

    The National Aeronautics and Space Administration (NASA) tasked The MITRE Corporation's Center for Advanced Aviation System Development (CAASD) to investigate potential air traffic control (ATC) procedures that could benefit from technology used or developed in NASA's Wake Vortex Advisory System (WakeVAS). The task also required developing an estimate of the potential benefits of the candidate procedures. The main thrust of the investigation was to evaluate opportunities for improved capacity and efficiency in airport arrival and departure operations. Other procedures that would provide safety enhancements were also considered. The purpose of this investigation was to provide input to the WakeVAS program office regarding the most promising areas of development for the program. A two-fold perspective was desired: First, identification of benefits from possible procedures enabled by both incremental components and the mature state of WakeVAS technology; second identification of procedures that could be expected to evolve from the current Federal Aviation Administration (FAA) procedures. The evolution of procedures should provide meaningful increments of benefit and a low risk implementation of the WakeVAS technologies.

  2. Survey of adaptive control using Liapunov design

    NASA Technical Reports Server (NTRS)

    Lindorff, D. P.; Carroll, R. L.

    1972-01-01

    A survey was made of the literature devoted to the synthesis of model-tracking adaptive systems based on application of Liapunov's second method. The basic synthesis procedure is introduced and a critical review of extensions made to the theory since 1966 is made. The extensions relate to design for relative stability, reduction of order techniques, design with disturbance, design with time variable parameters, multivariable systems, identification, and an adaptive observer.

  3. A Procedure for Evaluation of Dust Potential in Desert Terrains,

    DTIC Science & Technology

    1985-01-01

    Surficial Deposits, Silt, Clay, Sand. ABSTRACT ^•The evaluation of dust potential in hot deserts is based on the association of well de - fined dust...late Quaternary, which la heat understood, the efectiveaeas of climatic changes and the identification of euch •«ctuatioa in soils may he only roughly...ridges - ballenas . Nahal Roded, southern Arava Valley. „ ’ *m V J c^> ^ Mmmmmmmmmmmmmmmmw iu .......... . ^ ^^ - - _^^_^_m_ mm, PLATE S

  4. Pilot interaction with automated airborne decision making systems

    NASA Technical Reports Server (NTRS)

    Rouse, W. B.; Hammer, J. M.; Morris, N. M.; Knaeuper, A. E.; Brown, E. N.; Lewis, C. M.; Yoon, W. C.

    1984-01-01

    Two project areas were pursued: the intelligent cockpit and human problem solving. The first area involves an investigation of the use of advanced software engineering methods to aid aircraft crews in procedure selection and execution. The second area is focused on human problem solving in dynamic environments, particulary in terms of identification of rule-based models land alternative approaches to training and aiding. Progress in each area is discussed.

  5. A spin column-free approach to sodium hydroxide-based glycan permethylation.

    PubMed

    Hu, Yueming; Borges, Chad R

    2017-07-24

    Glycan permethylation was introduced as a tool to facilitate the study of glycans in 1903. Since that time, permethylation procedures have been continually modified to improve permethylation efficiency and qualitative applicability. Typically, however, either laborious preparation steps or cumbersome and uneconomical spin columns have been needed to obtain decent permethylation yields on small glycan samples. Here we describe a spin column-free (SCF) glycan permethylation procedure that is applicable to both O- and N-linked glycans and can be employed upstream to intact glycan analysis by MALDI-MS, ESI-MS, or glycan linkage analysis by GC-MS. The SCF procedure involves neutralization of NaOH beads by acidified phosphate buffer, which eliminates the risk of glycan oxidative degradation and avoids the use of spin columns. Optimization of the new permethylation procedure provided high permethylation efficiency for both hexose (>98%) and HexNAc (>99%) residues-yields which were comparable to (or better than) those of some widely-used spin column-based procedures. A light vs. heavy labelling approach was employed to compare intact glycan yields from a popular spin-column based approach to the SCF approach. Recovery of intact N-glycans was significantly better with the SCF procedure (p < 0.05), but overall yield of O-glycans was similar or slightly diminished (p < 0.05 for tetrasaccharides or smaller). When the SCF procedure was employed upstream to hydrolysis, reduction and acetylation for glycan linkage analysis of pooled glycans from unfractionated blood plasma, analytical reproducibility was on par with that from previous spin column-based "glycan node" analysis results. When applied to blood plasma samples from stage III-IV breast cancer patients (n = 20) and age-matched controls (n = 20), the SCF procedure facilitated identification of three glycan nodes with significantly different distributions between the cases and controls (ROC c-statistics > 0.75; p < 0.01). In summary, the SCF permethylation procedure expedites and economizes both intact glycan analysis and linkage analysis of glycans from whole biospecimens.

  6. A spin column-free approach to sodium hydroxide-based glycan permethylation†

    PubMed Central

    Hu, Yueming; Borges, Chad R.

    2018-01-01

    Glycan permethylation was introduced as a tool to facilitate the study of glycans in 1903. Since that time, permethylation procedures have been continually modified to improve permethylation efficiency and qualitative applicability. Typically, however, either laborious preparation steps or cumbersome and uneconomical spin columns have been needed to obtain decent permethylation yields on small glycan samples. Here we describe a spin column-free (SCF) glycan permethylation procedure that is applicable to both O- and N-linked glycans and can be employed upstream to intact glycan analysis by MALDI-MS, ESI-MS, or glycan linkage analysis by GC-MS. The SCF procedure involves neutralization of NaOH beads by acidified phosphate buffer, which eliminates the risk of glycan oxidative degradation and avoids the use of spin columns. Optimization of the new permethylation procedure provided high permethylation efficiency for both hexose (>98%) and HexNAc (>99%) residues—yields which were comparable to (or better than) those of some widely-used spin column-based procedures. A light vs. heavy labelling approach was employed to compare intact glycan yields from a popular spin-column based approach to the SCF approach. Recovery of intact N-glycans was significantly better with the SCF procedure (p < 0.05), but overall yield of O-glycans was similar or slightly diminished (p < 0.05 for tetrasaccharides or smaller). When the SCF procedure was employed upstream to hydrolysis, reduction and acetylation for glycan linkage analysis of pooled glycans from unfractionated blood plasma, analytical reproducibility was on par with that from previous spin column-based “glycan node” analysis results. When applied to blood plasma samples from stage III–IV breast cancer patients (n = 20) and age-matched controls (n = 20), the SCF procedure facilitated identification of three glycan nodes with significantly different distributions between the cases and controls (ROC c-statistics > 0.75; p < 0.01). In summary, the SCF permethylation procedure expedites and economizes both intact glycan analysis and linkage analysis of glycans from whole biospecimens. PMID:28635997

  7. The costs associated with adverse event procedures for an international HIV clinical trial determined by activity-based costing.

    PubMed

    Chou, Victoria B; Omer, Saad B; Hussain, Hamidah; Mugasha, Christine; Musisi, Maria; Mmiro, Francis; Musoke, Philippa; Jackson, J Brooks; Guay, Laura A

    2007-12-01

    To determine costs for adverse event (AE) procedures for a large HIV perinatal trial by analyzing actual resource consumption using activity-based costing (ABC) in an international research setting. The AE system for an ongoing clinical trial in Uganda was evaluated using ABC techniques to determine costs from the perspective of the study. Resources were organized into cost categories (eg, personnel, patient care expenses, laboratory testing, equipment). Cost drivers were quantified, and unit cost per AE was calculated. A subset of time and motion studies was performed prospectively to observe clinic personnel time required for AE identification. In 18 months, there were 9028 AEs, with 970 (11%) reported as serious adverse events. Unit cost per AE was $101.97. Overall, AE-related costs represented 32% ($920,581 of $2,834,692) of all study expenses. Personnel ($79.30) and patient care ($11.96) contributed the greatest proportion of component costs. Reported AEs were predominantly nonserious (mild or moderate severity) and unrelated to study drug(s) delivery. Intensive identification and management of AEs to conduct clinical trials ethically and protect human subjects require expenditure of substantial human and financial resources. Better understanding of these resource requirements should improve planning and funding of international HIV-related clinical trials.

  8. Identification of natural indigo in historical textiles by GC-MS.

    PubMed

    Degani, Laura; Riedo, Chiara; Chiantore, Oscar

    2015-02-01

    The possibility of successfully applying a common GC-MS procedure for identification in one step of all types of dyes from plants of unknown origin and from historical objects is particularly attractive due to the high separation efficiency of the capillary columns, the MS detection sensitivity and the reproducibility of results. In this work, GC-MS analysis, previously and successfully used for the characterization of anthraquinones, flavonoids and tannins from plant extracts and historical samples, has been tested on indigoid dyestuffs. An analytical procedure based on the silylating agent N,O-bis-(trimethylsilyl)trifluoroacetamide (BSTFA) with 1% trimethylchlorosilane (TMCS) was applied to pure molecules of indigotin and indirubin and to plant extracts of Indigofera tinctoria L. and Isatis tinctoria L. Preliminary tests have been done to establish the chromatographic conditions and the derivatization amounts most suitable for the simultaneous detection of indigoid molecules and of the other natural compounds, such as fatty acids, carboxylic acids and sugars, contained within the plant extracts. In order to assess the capacity and the sensitivity of the analytical procedure in typical archaeometric applications, wool samples dyed in the laboratory with indigo were analysed by mimicking the sample amounts typically available with historical objects. The electron ionization (EI) spectra of the main silylated derivatives of indigoid molecules obtained in this way constitute the necessary data set for the characterization of natural extracts and historical works of art. Subsequently, the procedure has been applied to historical samples for the detection of indigo and of other dyestuffs eventually contained in samples. Additional information, useful for restoration and preservation of works of art, could be also obtained on the nature of stains and smudges present on the sampled textile material. The GC-MS method turns out to be an efficient and fast analytical tool also for the identification of natural indigo in plants and textile artefacts, providing results complementary to those from high-performance liquid chromatography (HPLC).

  9. A Simple and Rapid Identification Method for Mycobacterium bovis BCG with Loop-Mediated Isothermal Amplification.

    PubMed

    Kouzaki, Yuji; Maeda, Takuya; Sasaki, Hiroaki; Tamura, Shinsuke; Hamamoto, Takaaki; Yuki, Atsushi; Sato, Akinori; Miyahira, Yasushi; Kawana, Akihiko

    2015-01-01

    Bacillus Calmette-Guérin (BCG) is widely used as a live attenuated vaccine against Mycobacterium tuberculosis and is an agent for standard prophylaxis against the recurrence of bladder cancer. Unfortunately, it can cause severe infectious diseases, especially in immunocompromised patients, and the ability to immediately distinguish BCG from other M. tuberculosis complexes is therefore important. In this study, we developed a simple and easy-to-perform identification procedure using loop-mediated amplification (LAMP) to detect deletions within the region of difference, which is deleted specifically in all M. bovis BCG strains. Reactions were performed at 64 °C for 30 min and successful targeted gene amplifications were detected by real-time turbidity using a turbidimeter and visual inspection of color change. The assay had an equivalent detection limit of 1.0 pg of genomic DNA using a turbidimeter whereas it was 10 pg with visual inspection, and it showed specificity against 49 strains of 44 pathogens, including M. tuberculosis complex. The expected LAMP products were confirmed through identical melting curves in real-time LAMP procedures. We employed the Procedure for Ultra Rapid Extraction (PURE) kit to isolate mycobacterial DNA and found that the highest sensitivity limit with a minimum total cell count of mycobacterium (including DNA purification with PURE) was up to 1 × 10(3) cells/reaction, based on color changes under natural light with FDA reagents. The detection limit of this procedure when applied to artificial serum, urine, cerebrospinal fluid, and bronchoalveolar lavage fluid samples was also about 1 × 10(3) cells/reaction. Therefore, this substitute method using conventional culture or clinical specimens followed by LAMP combined with PURE could be a powerful tool to enable the rapid identification of M. bovis BCG as point-of-care testing. It is suitable for practical use not only in resource-limited situations, but also in any clinical situation involving immunocompromised patients because of its convenience, rapidity, and cost effectiveness.

  10. A Simple and Rapid Identification Method for Mycobacterium bovis BCG with Loop-Mediated Isothermal Amplification

    PubMed Central

    Kouzaki, Yuji; Maeda, Takuya; Sasaki, Hiroaki; Tamura, Shinsuke; Hamamoto, Takaaki; Yuki, Atsushi; Sato, Akinori; Miyahira, Yasushi; Kawana, Akihiko

    2015-01-01

    Bacillus Calmette-Guérin (BCG) is widely used as a live attenuated vaccine against Mycobacterium tuberculosis and is an agent for standard prophylaxis against the recurrence of bladder cancer. Unfortunately, it can cause severe infectious diseases, especially in immunocompromised patients, and the ability to immediately distinguish BCG from other M. tuberculosis complexes is therefore important. In this study, we developed a simple and easy-to-perform identification procedure using loop-mediated amplification (LAMP) to detect deletions within the region of difference, which is deleted specifically in all M. bovis BCG strains. Reactions were performed at 64°C for 30 min and successful targeted gene amplifications were detected by real-time turbidity using a turbidimeter and visual inspection of color change. The assay had an equivalent detection limit of 1.0 pg of genomic DNA using a turbidimeter whereas it was 10 pg with visual inspection, and it showed specificity against 49 strains of 44 pathogens, including M. tuberculosis complex. The expected LAMP products were confirmed through identical melting curves in real-time LAMP procedures. We employed the Procedure for Ultra Rapid Extraction (PURE) kit to isolate mycobacterial DNA and found that the highest sensitivity limit with a minimum total cell count of mycobacterium (including DNA purification with PURE) was up to 1 × 103 cells/reaction, based on color changes under natural light with FDA reagents. The detection limit of this procedure when applied to artificial serum, urine, cerebrospinal fluid, and bronchoalveolar lavage fluid samples was also about 1 × 103 cells/reaction. Therefore, this substitute method using conventional culture or clinical specimens followed by LAMP combined with PURE could be a powerful tool to enable the rapid identification of M. bovis BCG as point-of-care testing. It is suitable for practical use not only in resource-limited situations, but also in any clinical situation involving immunocompromised patients because of its convenience, rapidity, and cost effectiveness. PMID:26208001

  11. 34 CFR 303.323 - Nondiscriminatory procedures.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... Services Identification and Evaluation § 303.323 Nondiscriminatory procedures. Each lead agency shall adopt... qualified personnel. (Approved by the Office of Management and Budget under control number 1820-0550...

  12. Microfluidic Separation of Circulating Tumor Cells Based on Size and Deformability.

    PubMed

    Park, Emily S; Duffy, Simon P; Ma, Hongshen

    2017-01-01

    Circulating tumor cells (CTCs) have been implicated as the seeds of cancer metastasis and therefore have the potential to provide significant prognostic and diagnostic values. Here, we describe a procedure for separating CTCs from whole blood based on size and deformability using the microfluidic ratchet device. This device leverages the ratcheting motion of single cells created as they are deformed through funnel-shaped constrictions using oscillatory flow in order to divert cells based on differences in size and deformability. Subsequent methods for CTC identification and enumeration using immunofluorescence after separation are also described.

  13. Immune-Response Patterns and Next Generation Sequencing Diagnostics for the Detection of Mycoses in Patients with Septic Shock—Results of a Combined Clinical and Experimental Investigation

    PubMed Central

    Decker, Sebastian O.; Sigl, Annette; Grumaz, Christian; Stevens, Philip; Vainshtein, Yevhen; Zimmermann, Stefan; Weigand, Markus A.; Hofer, Stefan; Sohn, Kai; Brenner, Thorsten

    2017-01-01

    Fungi are of increasing importance in sepsis. However, culture-based diagnostic procedures are associated with relevant weaknesses. Therefore, culture- and next-generation sequencing (NGS)-based fungal findings as well as corresponding plasma levels of β-d-glucan, interferon gamma (INF-γ), tumor necrosis factor alpha (TNF-α), interleukin (IL)-2, -4, -6, -10, -17A, and mid-regional proadrenomedullin (MR-proADM) were evaluated in 50 septic patients at six consecutive time points within 28 days after sepsis onset. Furthermore, immune-response patterns during infections with Candida spp. were studied in a reconstituted human epithelium model. In total, 22% (n = 11) of patients suffered from a fungal infection. An NGS-based diagnostic approach appeared to be suitable for the identification of fungal pathogens in patients suffering from fungemia as well as in patients with negative blood cultures. Moreover, MR-proADM and IL-17A in plasma proved suitable for the identification of patients with a fungal infection. Using RNA-seq., adrenomedullin (ADM) was shown to be a target gene which is upregulated early after an epithelial infection with Candida spp. In summary, an NGS-based diagnostic approach was able to close the diagnostic gap of routinely used culture-based diagnostic procedures, which can be further facilitated by plasmatic measurements of MR-proADM and IL-17A. In addition, ADM was identified as an early target gene in response to epithelial infections with Candida spp. PMID:28820494

  14. 32 CFR 643.22 - Policy-Public safety: Requirement for early identification of lands containing dangerous materials.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... identification of lands containing dangerous materials. 643.22 Section 643.22 National Defense Department of...—Public safety: Requirement for early identification of lands containing dangerous materials. (a) DA will... lands by explosives, military chemical or other dangerous materials. (c) Procedures with respect to...

  15. 32 CFR 643.22 - Policy-Public safety: Requirement for early identification of lands containing dangerous materials.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... identification of lands containing dangerous materials. 643.22 Section 643.22 National Defense Department of...—Public safety: Requirement for early identification of lands containing dangerous materials. (a) DA will... lands by explosives, military chemical or other dangerous materials. (c) Procedures with respect to...

  16. 32 CFR 643.22 - Policy-Public safety: Requirement for early identification of lands containing dangerous materials.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... identification of lands containing dangerous materials. 643.22 Section 643.22 National Defense Department of...—Public safety: Requirement for early identification of lands containing dangerous materials. (a) DA will... lands by explosives, military chemical or other dangerous materials. (c) Procedures with respect to...

  17. 75 FR 32295 - Expedited Approval of Alternative Test Procedures for the Analysis of Contaminants Under the Safe...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-06-08

    ... Identification of Coliform Bacteria and Escherichia coli in Finished Waters, January 2007, Version 1.1... Membrane Filter Test Method for Detection and Identification of Coliform Bacteria and Escherichia coli in... Detection and Identification of Coliform Bacteria and Escherichia coli in Finished Waters. November, 2000...

  18. Manual LANDSAT data analysis for crop type identification

    NASA Technical Reports Server (NTRS)

    Hay, C. M. (Principal Investigator)

    1979-01-01

    The process of manual identification of crop type by human analysts and problems associated in LACIE that were associated with manual crop identification measurement procedures are described. Research undertaken in cooperation with LACIE operations by the supporting research community to effect solutions to, or obtain greater understanding of the problems is discussed.

  19. Qualitative Analysis by Gas Chromatography: GC versus the Nose in Formulating Artificial Fruit Flavors.

    ERIC Educational Resources Information Center

    Rasmussen, P. W.

    1984-01-01

    Describes an undergraduate laboratory experiment used to illustrate the use of gas chromatography retention indices for the identification of unknown compounds, specifically for the identification of unknown compounds and for the identification of the volatile compounds responsible for the odor of the banana. Procedures, reference data, and sample…

  20. 76 FR 5843 - Self-Regulatory Organizations; The Depository Trust Company; Notice of Filing and Order Granting...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-02-02

    ... release of the White Paper, DTC customers requested a tool that would help measure the impact of the... corresponding CUSIP-level identification information at 8:20 a.m.; and (3) make other conforming changes. II... on Uniform Security Identification Procedures (``CUSIP'') level identification information about the...

  1. Probability of identification (POI): a statistical model for the validation of qualitative botanical identification methods

    USDA-ARS?s Scientific Manuscript database

    A qualitative botanical identification method (BIM) is an analytical procedure which returns a binary result (1 = Identified, 0 = Not Identified). A BIM may be used by a buyer, manufacturer, or regulator to determine whether a botanical material being tested is the same as the target (desired) mate...

  2. Virtual human versus human administration of photographic lineups.

    PubMed

    Daugherty, Brent; Babu, Sabarish; Wallendael, Lori Van; Cutler, Brian; Hodges, Larry F

    2008-01-01

    One solution to mistaken identification by a crime's victims and eyewitnesses is to use a virtual officer to conduct identification procedures. Results from a study comparing a virtual officer with a live human investigator indicate that the virtual officer performs comparably to the human in terms of identification accuracy, emotional affect, and ease of use.

  3. 37 CFR 212.5 - Recordation of distinctive identification of vessel hull designer.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... identification of vessel hull designer. 212.5 Section 212.5 Patents, Trademarks, and Copyrights COPYRIGHT OFFICE, LIBRARY OF CONGRESS COPYRIGHT OFFICE AND PROCEDURES PROTECTION OF VESSEL HULL DESIGNS § 212.5 Recordation of distinctive identification of vessel hull designer. (a) General. Any owner of a vessel hull may...

  4. 10 CFR 707.7 - Random drug testing requirements and identification of testing designated positions.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 10 Energy 4 2012-01-01 2012-01-01 false Random drug testing requirements and identification of... PROGRAMS AT DOE SITES Procedures § 707.7 Random drug testing requirements and identification of testing... evidence of the use of illegal drugs of employees in testing designated positions identified in this...

  5. 10 CFR 707.7 - Random drug testing requirements and identification of testing designated positions.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 10 Energy 4 2014-01-01 2014-01-01 false Random drug testing requirements and identification of... PROGRAMS AT DOE SITES Procedures § 707.7 Random drug testing requirements and identification of testing... evidence of the use of illegal drugs of employees in testing designated positions identified in this...

  6. 10 CFR 707.7 - Random drug testing requirements and identification of testing designated positions.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 10 Energy 4 2011-01-01 2011-01-01 false Random drug testing requirements and identification of... PROGRAMS AT DOE SITES Procedures § 707.7 Random drug testing requirements and identification of testing... evidence of the use of illegal drugs of employees in testing designated positions identified in this...

  7. 10 CFR 707.7 - Random drug testing requirements and identification of testing designated positions.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 10 Energy 4 2013-01-01 2013-01-01 false Random drug testing requirements and identification of... PROGRAMS AT DOE SITES Procedures § 707.7 Random drug testing requirements and identification of testing... evidence of the use of illegal drugs of employees in testing designated positions identified in this...

  8. 10 CFR 707.7 - Random drug testing requirements and identification of testing designated positions.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 10 Energy 4 2010-01-01 2010-01-01 false Random drug testing requirements and identification of... PROGRAMS AT DOE SITES Procedures § 707.7 Random drug testing requirements and identification of testing... evidence of the use of illegal drugs of employees in testing designated positions identified in this...

  9. Dynamic Stiffness Transfer Function of an Electromechanical Actuator Using System Identification

    NASA Astrophysics Data System (ADS)

    Kim, Sang Hwa; Tahk, Min-Jea

    2018-04-01

    In the aeroelastic analysis of flight vehicles with electromechanical actuators (EMAs), an accurate prediction of flutter requires dynamic stiffness characteristics of the EMA. The dynamic stiffness transfer function of the EMA with brushless direct current (BLDC) motor can be obtained by conducting complicated mathematical calculations of control algorithms and mechanical/electrical nonlinearities using linearization techniques. Thus, system identification approaches using experimental data, as an alternative, have considerable advantages. However, the test setup for system identification is expensive and complex, and experimental procedures for data collection are time-consuming tasks. To obtain the dynamic stiffness transfer function, this paper proposes a linear system identification method that uses information obtained from a reliable dynamic stiffness model with a control algorithm and nonlinearities. The results of this study show that the system identification procedure is compact, and the transfer function is able to describe the dynamic stiffness characteristics of the EMA. In addition, to verify the validity of the system identification method, the simulation results of the dynamic stiffness transfer function and the dynamic stiffness model were compared with the experimental data for various external loads.

  10. Vortex identification from local properties of the vorticity field

    NASA Astrophysics Data System (ADS)

    Elsas, J. H.; Moriconi, L.

    2017-01-01

    A number of systematic procedures for the identification of vortices/coherent structures have been developed as a way to address their possible kinematical and dynamical roles in structural formulations of turbulence. It has been broadly acknowledged, however, that vortex detection algorithms, usually based on linear-algebraic properties of the velocity gradient tensor, can be plagued with severe shortcomings and may become, in practical terms, dependent on the choice of subjective threshold parameters in their implementations. In two-dimensions, a large class of standard vortex identification prescriptions turn out to be equivalent to the "swirling strength criterion" (λc i-criterion), which is critically revisited in this work. We classify the instances where the accuracy of the λc i-criterion is affected by nonlinear superposition effects and propose an alternative vortex detection scheme based on the local curvature properties of the vorticity graph (x ,y ,ω ) —the "vorticity curvature criterion" (λω-criterion)—which improves over the results obtained with the λc i-criterion in controlled Monte Carlo tests. A particularly problematic issue, given its importance in wall-bounded flows, is the eventual inadequacy of the λc i-criterion for many-vortex configurations in the presence of strong background shear. We show that the λω-criterion is able to cope with these cases as well, if a subtraction of the mean velocity field background is performed, in the spirit of the Reynolds decomposition procedure. A realistic comparative study for vortex identification is then carried out for a direct numerical simulation of a turbulent channel flow, including a three-dimensional extension of the λω-criterion. In contrast to the λc i-criterion, the λω-criterion indicates in a consistent way the existence of small scale isotropic turbulent fluctuations in the logarithmic layer, in consonance with long-standing assumptions commonly taken in turbulent boundary layer phenomenology.

  11. Grey-box state-space identification of nonlinear mechanical vibrations

    NASA Astrophysics Data System (ADS)

    Noël, J. P.; Schoukens, J.

    2018-05-01

    The present paper deals with the identification of nonlinear mechanical vibrations. A grey-box, or semi-physical, nonlinear state-space representation is introduced, expressing the nonlinear basis functions using a limited number of measured output variables. This representation assumes that the observed nonlinearities are localised in physical space, which is a generic case in mechanics. A two-step identification procedure is derived for the grey-box model parameters, integrating nonlinear subspace initialisation and weighted least-squares optimisation. The complete procedure is applied to an electrical circuit mimicking the behaviour of a single-input, single-output (SISO) nonlinear mechanical system and to a single-input, multiple-output (SIMO) geometrically nonlinear beam structure.

  12. Identification of environmental sounds and melodies in syndromes of anterior temporal lobe degeneration.

    PubMed

    Golden, Hannah L; Downey, Laura E; Fletcher, Philip D; Mahoney, Colin J; Schott, Jonathan M; Mummery, Catherine J; Crutch, Sebastian J; Warren, Jason D

    2015-05-15

    Recognition of nonverbal sounds in semantic dementia and other syndromes of anterior temporal lobe degeneration may determine clinical symptoms and help to define phenotypic profiles. However, nonverbal auditory semantic function has not been widely studied in these syndromes. Here we investigated semantic processing in two key nonverbal auditory domains - environmental sounds and melodies - in patients with semantic dementia (SD group; n=9) and in patients with anterior temporal lobe atrophy presenting with behavioural decline (TL group; n=7, including four cases with MAPT mutations) in relation to healthy older controls (n=20). We assessed auditory semantic performance in each domain using novel, uniform within-modality neuropsychological procedures that determined sound identification based on semantic classification of sound pairs. Both the SD and TL groups showed comparable overall impairments of environmental sound and melody identification; individual patients generally showed superior identification of environmental sounds than melodies, however relative sparing of melody over environmental sound identification also occurred in both groups. Our findings suggest that nonverbal auditory semantic impairment is a common feature of neurodegenerative syndromes with anterior temporal lobe atrophy. However, the profile of auditory domain involvement varies substantially between individuals. Copyright © 2015. Published by Elsevier B.V.

  13. Rotor dynamic simulation and system identification methods for application to vacuum whirl data

    NASA Technical Reports Server (NTRS)

    Berman, A.; Giansante, N.; Flannelly, W. G.

    1980-01-01

    Methods of using rotor vacuum whirl data to improve the ability to model helicopter rotors were developed. The work consisted of the formulation of the equations of motion of elastic blades on a hub using a Galerkin method; the development of a general computer program for simulation of these equations; the study and implementation of a procedure for determining physical parameters based on measured data; and the application of a method for computing the normal modes and natural frequencies based on test data.

  14. Differentiation of five body fluids from forensic samples by expression analysis of four microRNAs using quantitative PCR.

    PubMed

    Sauer, Eva; Reinke, Ann-Kathrin; Courts, Cornelius

    2016-05-01

    Applying molecular genetic approaches for the identification of forensically relevant body fluids, which often yield crucial information for the reconstruction of a potential crime, is a current topic of forensic research. Due to their body fluid specific expression patterns and stability against degradation, microRNAs (miRNA) emerged as a promising molecular species, with a range of candidate markers published. The analysis of miRNA via quantitative Real-Time PCR, however, should be based on a relevant strategy of normalization of non-biological variances to deliver reliable and biologically meaningful results. The herein presented work is the as yet most comprehensive study of forensic body fluid identification via miRNA expression analysis based on a thoroughly validated qPCR procedure and unbiased statistical decision making to identify single source samples. Copyright © 2016 Elsevier Ireland Ltd. All rights reserved.

  15. Modal parameter identification of a CMUT membrane using response data only

    NASA Astrophysics Data System (ADS)

    Lardiès, Joseph; Bourbon, Gilles; Moal, Patrice Le; Kacem, Najib; Walter, Vincent; Le, Thien-Phu

    2018-03-01

    Capacitive micromachined ultrasonic transducers (CMUTs) are microelectromechanical systems used for the generation of ultrasounds. The fundamental element of the transducer is a clamped thin metallized membrane that vibrates under voltage variations. To control such oscillations and to optimize its dynamic response it is necessary to know the modal parameters of the membrane such as resonance frequency, damping and stiffness coefficients. The purpose of this work is to identify these parameters using only the time data obtained from the membrane center displacement. Dynamic measurements are conducted in time domain and we use two methods to identify the modal parameters: a subspace method based on an innovation model of the state-space representation and the continuous wavelet transform method based on the use of the ridge of the wavelet transform of the displacement. Experimental results are presented showing the effectiveness of these two procedures in modal parameter identification.

  16. Application of DNA-based methods in forensic entomology.

    PubMed

    Wells, Jeffrey D; Stevens, Jamie R

    2008-01-01

    A forensic entomological investigation can benefit from a variety of widely practiced molecular genotyping methods. The most commonly used is DNA-based specimen identification. Other applications include the identification of insect gut contents and the characterization of the population genetic structure of a forensically important insect species. The proper application of these procedures demands that the analyst be technically expert. However, one must also be aware of the extensive list of standards and expectations that many legal systems have developed for forensic DNA analysis. We summarize the DNA techniques that are currently used in, or have been proposed for, forensic entomology and review established genetic analyses from other scientific fields that address questions similar to those in forensic entomology. We describe how accepted standards for forensic DNA practice and method validation are likely to apply to insect evidence used in a death or other forensic entomological investigation.

  17. Use of the Morlet mother wavelet in the frequency-scale domain decomposition technique for the modal identification of ambient vibration responses

    NASA Astrophysics Data System (ADS)

    Le, Thien-Phu

    2017-10-01

    The frequency-scale domain decomposition technique has recently been proposed for operational modal analysis. The technique is based on the Cauchy mother wavelet. In this paper, the approach is extended to the Morlet mother wavelet, which is very popular in signal processing due to its superior time-frequency localization. Based on the regressive form and an appropriate norm of the Morlet mother wavelet, the continuous wavelet transform of the power spectral density of ambient responses enables modes in the frequency-scale domain to be highlighted. Analytical developments first demonstrate the link between modal parameters and the local maxima of the continuous wavelet transform modulus. The link formula is then used as the foundation of the proposed modal identification method. Its practical procedure, combined with the singular value decomposition algorithm, is presented step by step. The proposition is finally verified using numerical examples and a laboratory test.

  18. Fault-tolerant optimised tracking control for unknown discrete-time linear systems using a combined reinforcement learning and residual compensation methodology

    NASA Astrophysics Data System (ADS)

    Han, Ke-Zhen; Feng, Jian; Cui, Xiaohong

    2017-10-01

    This paper considers the fault-tolerant optimised tracking control (FTOTC) problem for unknown discrete-time linear system. A research scheme is proposed on the basis of data-based parity space identification, reinforcement learning and residual compensation techniques. The main characteristic of this research scheme lies in the parity-space-identification-based simultaneous tracking control and residual compensation. The specific technical line consists of four main contents: apply subspace aided method to design observer-based residual generator; use reinforcement Q-learning approach to solve optimised tracking control policy; rely on robust H∞ theory to achieve noise attenuation; adopt fault estimation triggered by residual generator to perform fault compensation. To clarify the design and implementation procedures, an integrated algorithm is further constructed to link up these four functional units. The detailed analysis and proof are subsequently given to explain the guaranteed FTOTC performance of the proposed conclusions. Finally, a case simulation is provided to verify its effectiveness.

  19. Output-only modal dynamic identification of frames by a refined FDD algorithm at seismic input and high damping

    NASA Astrophysics Data System (ADS)

    Pioldi, Fabio; Ferrari, Rosalba; Rizzi, Egidio

    2016-02-01

    The present paper deals with the seismic modal dynamic identification of frame structures by a refined Frequency Domain Decomposition (rFDD) algorithm, autonomously formulated and implemented within MATLAB. First, the output-only identification technique is outlined analytically and then employed to characterize all modal properties. Synthetic response signals generated prior to the dynamic identification are adopted as input channels, in view of assessing a necessary condition for the procedure's efficiency. Initially, the algorithm is verified on canonical input from random excitation. Then, modal identification has been attempted successfully at given seismic input, taken as base excitation, including both strong motion data and single and multiple input ground motions. Rather than different attempts investigating the role of seismic response signals in the Time Domain, this paper considers the identification analysis in the Frequency Domain. Results turn-out very much consistent with the target values, with quite limited errors in the modal estimates, including for the damping ratios, ranging from values in the order of 1% to 10%. Either seismic excitation and high values of damping, resulting critical also in case of well-spaced modes, shall not fulfill traditional FFD assumptions: this shows the consistency of the developed algorithm. Through original strategies and arrangements, the paper shows that a comprehensive rFDD modal dynamic identification of frames at seismic input is feasible, also at concomitant high damping.

  20. A framework for assessing the adequacy and effectiveness of software development methodologies

    NASA Technical Reports Server (NTRS)

    Arthur, James D.; Nance, Richard E.

    1990-01-01

    Tools, techniques, environments, and methodologies dominate the software engineering literature, but relatively little research in the evaluation of methodologies is evident. This work reports an initial attempt to develop a procedural approach to evaluating software development methodologies. Prominent in this approach are: (1) an explication of the role of a methodology in the software development process; (2) the development of a procedure based on linkages among objectives, principles, and attributes; and (3) the establishment of a basis for reduction of the subjective nature of the evaluation through the introduction of properties. An application of the evaluation procedure to two Navy methodologies has provided consistent results that demonstrate the utility and versatility of the evaluation procedure. Current research efforts focus on the continued refinement of the evaluation procedure through the identification and integration of product quality indicators reflective of attribute presence, and the validation of metrics supporting the measure of those indicators. The consequent refinement of the evaluation procedure offers promise of a flexible approach that admits to change as the field of knowledge matures. In conclusion, the procedural approach presented in this paper represents a promising path toward the end goal of objectively evaluating software engineering methodologies.

  1. A methodological, task-based approach to Procedure-Specific Simulations training.

    PubMed

    Setty, Yaki; Salzman, Oren

    2016-12-01

    Procedure-Specific Simulations (PSS) are 3D realistic simulations that provide a platform to practice complete surgical procedures in a virtual-reality environment. While PSS have the potential to improve surgeons' proficiency, there are no existing standards or guidelines for PSS development in a structured manner. We employ a unique platform inspired by game design to develop virtual reality simulations in three dimensions of urethrovesical anastomosis during radical prostatectomy. 3D visualization is supported by a stereo vision, providing a fully realistic view of the simulation. The software can be executed for any robotic surgery platform. Specifically, we tested the simulation under windows environment on the RobotiX Mentor. Using urethrovesical anastomosis during radical prostatectomy simulation as a representative example, we present a task-based methodological approach to PSS training. The methodology provides tasks in increasing levels of difficulty from a novice level of basic anatomy identification, to an expert level that permits testing new surgical approaches. The modular methodology presented here can be easily extended to support more complex tasks. We foresee this methodology as a tool used to integrate PSS as a complementary training process for surgical procedures.

  2. Design and Mechanical Evaluation of a Capacitive Sensor-Based Indexed Platform for Verification of Portable Coordinate Measuring Instruments

    PubMed Central

    Avila, Agustín Brau; Mazo, Jorge Santolaria; Martín, Juan José Aguilar

    2014-01-01

    During the last years, the use of Portable Coordinate Measuring Machines (PCMMs) in industry has increased considerably, mostly due to their flexibility for accomplishing in-line measuring tasks as well as their reduced costs and operational advantages as compared to traditional coordinate measuring machines (CMMs). However, their operation has a significant drawback derived from the techniques applied in the verification and optimization procedures of their kinematic parameters. These techniques are based on the capture of data with the measuring instrument from a calibrated gauge object, fixed successively in various positions so that most of the instrument measuring volume is covered, which results in time-consuming, tedious and expensive verification procedures. In this work the mechanical design of an indexed metrology platform (IMP) is presented. The aim of the IMP is to increase the final accuracy and to radically simplify the calibration, identification and verification of geometrical parameter procedures of PCMMs. The IMP allows us to fix the calibrated gauge object and move the measuring instrument in such a way that it is possible to cover most of the instrument working volume, reducing the time and operator fatigue to carry out these types of procedures. PMID:24451458

  3. Design and mechanical evaluation of a capacitive sensor-based indexed platform for verification of portable coordinate measuring instruments.

    PubMed

    Avila, Agustín Brau; Mazo, Jorge Santolaria; Martín, Juan José Aguilar

    2014-01-02

    During the last years, the use of Portable Coordinate Measuring Machines (PCMMs) in industry has increased considerably, mostly due to their flexibility for accomplishing in-line measuring tasks as well as their reduced costs and operational advantages as compared to traditional coordinate measuring machines (CMMs). However, their operation has a significant drawback derived from the techniques applied in the verification and optimization procedures of their kinematic parameters. These techniques are based on the capture of data with the measuring instrument from a calibrated gauge object, fixed successively in various positions so that most of the instrument measuring volume is covered, which results in time-consuming, tedious and expensive verification procedures. In this work the mechanical design of an indexed metrology platform (IMP) is presented. The aim of the IMP is to increase the final accuracy and to radically simplify the calibration, identification and verification of geometrical parameter procedures of PCMMs. The IMP allows us to fix the calibrated gauge object and move the measuring instrument in such a way that it is possible to cover most of the instrument working volume, reducing the time and operator fatigue to carry out these types of procedures.

  4. Post-procedural Care in Interventional Radiology: What Every Interventional Radiologist Should Know-Part II: Catheter Care and Management of Common Systemic Post-procedural Complications.

    PubMed

    Taslakian, Bedros; Sridhar, Divya

    2017-09-01

    Interventional radiology (IR) has evolved into a full-fledged clinical specialty with attendant comprehensive patient care responsibilities. Providing excellent and thorough clinical care is as essential to the practice of IR as achieving technical success in procedures. Basic clinical skills that every interventional radiologist should learn include routine management of percutaneously inserted drainage and vascular catheters and rapid effective management of common systemic post-procedural complications. A structured approach to post-procedural care, including routine follow-up and early identification and management of complications, facilitates efficient and thorough management with an emphasis on quality and patient safety. The aim of this second part, in conjunction with part 1, is to complete the comprehensive review of post-procedural care in patients undergoing interventional radiology procedures. We discuss common problems encountered after insertion of drainage and vascular catheters and describe effective methods of troubleshooting these problems. Commonly encountered systemic complications in IR are described, and ways for immediate identification and management of these complications are provided.

  5. Competency-based medical education for plastic surgery: where do we begin?

    PubMed

    Knox, Aaron D C; Gilardino, Mirko S; Kasten, Steve J; Warren, Richard J; Anastakis, Dimitri J

    2014-05-01

    North American surgical education is beginning to shift toward competency-based medical education, in which trainees complete their training only when competence has been demonstrated through objective milestones. Pressure is mounting to embrace competency-based medical education because of the perception that it provides more transparent standards and increased public accountability. In response to calls for reform from leading bodies in medical education, competency-based medical education is rapidly becoming the standard in training of physicians. The authors summarize the rationale behind the recent shift toward competency-based medical education and creation of the milestones framework. With respect to procedural skills, initial efforts will require the field of plastic surgery to overcome three challenges: identifying competencies (principles and procedures), modeling teaching strategies, and developing assessment tools. The authors provide proposals for how these challenges may be addressed and the educational rationale behind each proposal. A framework for identification of competencies and a stepwise approach toward creation of a principles oriented competency-based medical education curriculum for plastic surgery are presented. An assessment matrix designed to sample resident exposure to core principles and key procedures is proposed, along with suggestions for generating validity evidence for assessment tools. The ideal curriculum should provide exposure to core principles of plastic surgery while demonstrating competence through performance of index procedures that are most likely to benefit graduating residents when entering independent practice and span all domains of plastic surgery. The authors advocate that exploring the role and potential benefits of competency-based medical education in plastic surgery residency training is timely.

  6. Mobile visual object identification: from SIFT-BoF-RANSAC to Sketchprint

    NASA Astrophysics Data System (ADS)

    Voloshynovskiy, Sviatoslav; Diephuis, Maurits; Holotyak, Taras

    2015-03-01

    Mobile object identification based on its visual features find many applications in the interaction with physical objects and security. Discriminative and robust content representation plays a central role in object and content identification. Complex post-processing methods are used to compress descriptors and their geometrical information, aggregate them into more compact and discriminative representations and finally re-rank the results based on the similarity geometries of descriptors. Unfortunately, most of the existing descriptors are not very robust and discriminative once applied to the various contend such as real images, text or noise-like microstructures next to requiring at least 500-1'000 descriptors per image for reliable identification. At the same time, the geometric re-ranking procedures are still too complex to be applied to the numerous candidates obtained from the feature similarity based search only. This restricts that list of candidates to be less than 1'000 which obviously causes a higher probability of miss. In addition, the security and privacy of content representation has become a hot research topic in multimedia and security communities. In this paper, we introduce a new framework for non- local content representation based on SketchPrint descriptors. It extends the properties of local descriptors to a more informative and discriminative, yet geometrically invariant content representation. In particular it allows images to be compactly represented by 100 SketchPrint descriptors without being fully dependent on re-ranking methods. We consider several use cases, applying SketchPrint descriptors to natural images, text documents, packages and micro-structures and compare them with the traditional local descriptors.

  7. A Brief Taxometrics Primer

    PubMed Central

    Beauchaine, Theodore P.

    2009-01-01

    Taxometric procedures provide an empirical means of determining which psychiatric disorders are typologically distinct from normal behavioral functioning. Although most disorders reflect extremes along continuously distributed behavioral traits, identifying those that are discrete has important implications for accurate diagnosis, effective treatment, early identification of risk, and improved understanding of etiology. This article provides (a) brief descriptions of the conceptual bases of several taxometric procedures, (b) example analyses using simulated data, and (c) strategies for avoiding common pitfalls that are often observed in taxometrics research. To date, most taxometrics studies have appeared in the adult psychopathology literature. It is hoped that this primer will encourage interested readers to extend taxometrics research to child and adolescent populations. PMID:18088222

  8. Identification of illicit drugs by a combination of liquid chromatography and surface-enhanced Raman scattering spectroscopy

    NASA Astrophysics Data System (ADS)

    Sägmüller, Bernd; Schwarze, Bernd; Brehm, Georg; Trachta, Gerd; Schneider, Siegfried

    2003-12-01

    We have developed a new analysis procedure based upon High-Performance Liquid Chromatography (HPLC) in combination with surface-enhanced Raman scattering (SERS) spectroscopy as detection technique to meet todays need for an additional unique and reliable identification method of the ingredients of illicitly sold drugs or other pharmaceutical compounds. Separation of the individual components of a sample was preferentially achieved by employing an acetonitrile free eluent. The fractions of interest were collected as microliter volumes in the wells of a microtiter plate, which contained a home-made, matrix-stabilized silver halide dispersion. The latter functions as the precursor for the SERS-active surface generated by the probing laser beam. The limits of detection can be as low as 1 μg of analyte per one well of the microtiter plate. The recorded SERS spectra of the drugs Cocaine, Heroine and Amphetamine or the pharmaceuticals (Nor-) Papaverine and Procaine promise the possibility of a unique identification, especially if compared with the spectra of reference samples, and, therefore, can support the conclusions drawn by other identification techniques, if requested for example during a law suit.

  9. Computational system identification of continuous-time nonlinear systems using approximate Bayesian computation

    NASA Astrophysics Data System (ADS)

    Krishnanathan, Kirubhakaran; Anderson, Sean R.; Billings, Stephen A.; Kadirkamanathan, Visakan

    2016-11-01

    In this paper, we derive a system identification framework for continuous-time nonlinear systems, for the first time using a simulation-focused computational Bayesian approach. Simulation approaches to nonlinear system identification have been shown to outperform regression methods under certain conditions, such as non-persistently exciting inputs and fast-sampling. We use the approximate Bayesian computation (ABC) algorithm to perform simulation-based inference of model parameters. The framework has the following main advantages: (1) parameter distributions are intrinsically generated, giving the user a clear description of uncertainty, (2) the simulation approach avoids the difficult problem of estimating signal derivatives as is common with other continuous-time methods, and (3) as noted above, the simulation approach improves identification under conditions of non-persistently exciting inputs and fast-sampling. Term selection is performed by judging parameter significance using parameter distributions that are intrinsically generated as part of the ABC procedure. The results from a numerical example demonstrate that the method performs well in noisy scenarios, especially in comparison to competing techniques that rely on signal derivative estimation.

  10. A photometric mode identification method, including an improved non-adiabatic treatment of the atmosphere

    NASA Astrophysics Data System (ADS)

    Dupret, M.-A.; De Ridder, J.; De Cat, P.; Aerts, C.; Scuflaire, R.; Noels, A.; Thoul, A.

    2003-02-01

    We present an improved version of the method of photometric mode identification of Heynderickx et al. (\\cite{hey}). Our new version is based on the inclusion of precise non-adiabatic eigenfunctions determined in the outer stellar atmosphere according to the formalism recently proposed by Dupret et al. (\\cite{dup}). Our improved photometric mode identification technique is therefore no longer dependent on ad hoc parameters for the non-adiabatic effects. It contains the complete physical conditions of the outer atmosphere of the star, provided that rotation does not play a key role. We apply our method to the two slowly pulsating B stars HD 74560 and HD 138764 and to the beta Cephei star EN (16) Lac. Besides identifying the degree l of the pulsating stars, our method is also a tool for improving the knowledge of stellar interiors and atmospheres, by imposing constraints on parameters such as the metallicity and the mixing-length parameter alpha (a procedure we label non-adiabatic asteroseismology). The non-adiabatic eigenfunctions needed for the mode identification are available upon request from the authors.

  11. Ares I-X In-Flight Modal Identification

    NASA Technical Reports Server (NTRS)

    Bartkowicz, Theodore J.; James, George H., III

    2011-01-01

    Operational modal analysis is a procedure that allows the extraction of modal parameters of a structure in its operating environment. It is based on the idealized premise that input to the structure is white noise. In some cases, when free decay responses are corrupted by unmeasured random disturbances, the response data can be processed into cross-correlation functions that approximate free decay responses. Modal parameters can be computed from these functions by time domain identification methods such as the Eigenvalue Realization Algorithm (ERA). The extracted modal parameters have the same characteristics as impulse response functions of the original system. Operational modal analysis is performed on Ares I-X in-flight data. Since the dynamic system is not stationary due to propellant mass loss, modal identification is only possible by analyzing the system as a series of linearized models over short periods of time via a sliding time-window of short time intervals. A time-domain zooming technique was also employed to enhance the modal parameter extraction. Results of this study demonstrate that free-decay time domain modal identification methods can be successfully employed for in-flight launch vehicle modal extraction.

  12. 40 CFR 241.3 - Standards and procedures for identification of non-hazardous secondary materials that are solid...

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... identification of non-hazardous secondary materials that are solid wastes when used as fuels or ingredients in...) SOLID WASTES SOLID WASTES USED AS FUELS OR INGREDIENTS IN COMBUSTION UNITS Identification of Non-Hazardous Secondary Materials That Are Solid Wastes When Used as Fuels or Ingredients in Combustion Units...

  13. 37 CFR 212.5 - Recordation of distinctive identification of vessel hull designer.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ..., LIBRARY OF CONGRESS COPYRIGHT OFFICE AND PROCEDURES PROTECTION OF VESSEL HULL DESIGNS § 212.5 Recordation... distinctive identification in a design protection notice required by 17 U.S.C. 1306. A distinctive identification of an owner may not be used in a design notice before it has first been recorded with the Register...

  14. 37 CFR 212.5 - Recordation of distinctive identification of vessel hull designer.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ..., LIBRARY OF CONGRESS COPYRIGHT OFFICE AND PROCEDURES PROTECTION OF VESSEL HULL DESIGNS § 212.5 Recordation... distinctive identification in a design protection notice required by 17 U.S.C. 1306. A distinctive identification of an owner may not be used in a design notice before it has first been recorded with the Register...

  15. 37 CFR 212.5 - Recordation of distinctive identification of vessel hull designer.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ..., LIBRARY OF CONGRESS COPYRIGHT OFFICE AND PROCEDURES PROTECTION OF VESSEL HULL DESIGNS § 212.5 Recordation... distinctive identification in a design protection notice required by 17 U.S.C. 1306. A distinctive identification of an owner may not be used in a design notice before it has first been recorded with the Register...

  16. 37 CFR 212.5 - Recordation of distinctive identification of vessel hull designer.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... OFFICE, LIBRARY OF CONGRESS COPYRIGHT OFFICE AND PROCEDURES PROTECTION OF VESSEL HULL DESIGNS § 212.5... distinctive identification in a design protection notice required by 17 U.S.C. 1306. A distinctive identification of an owner may not be used in a design notice before it has first been recorded with the Register...

  17. 49 CFR 220.27 - Identification.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 49 Transportation 4 2014-10-01 2014-10-01 false Identification. 220.27 Section 220.27 Transportation Other Regulations Relating to Transportation (Continued) FEDERAL RAILROAD ADMINISTRATION, DEPARTMENT OF TRANSPORTATION RAILROAD COMMUNICATIONS Radio and Wireless Communication Procedures § 220.27...

  18. 49 CFR 220.27 - Identification.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 49 Transportation 4 2013-10-01 2013-10-01 false Identification. 220.27 Section 220.27 Transportation Other Regulations Relating to Transportation (Continued) FEDERAL RAILROAD ADMINISTRATION, DEPARTMENT OF TRANSPORTATION RAILROAD COMMUNICATIONS Radio and Wireless Communication Procedures § 220.27...

  19. 49 CFR 220.27 - Identification.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 49 Transportation 4 2010-10-01 2010-10-01 false Identification. 220.27 Section 220.27 Transportation Other Regulations Relating to Transportation (Continued) FEDERAL RAILROAD ADMINISTRATION, DEPARTMENT OF TRANSPORTATION RAILROAD COMMUNICATIONS Radio and Wireless Communication Procedures § 220.27...

  20. Computer method for identification of boiler transfer functions

    NASA Technical Reports Server (NTRS)

    Miles, J. H.

    1972-01-01

    Iterative computer aided procedure was developed which provides for identification of boiler transfer functions using frequency response data. Method uses frequency response data to obtain satisfactory transfer function for both high and low vapor exit quality data.

  1. Identification of Intellectually Able Disadvantaged Filipino Children.

    ERIC Educational Resources Information Center

    Naval-Severino, Teresita

    1992-01-01

    Preschool Filipino children from disadvantaged urban communities were assessed for giftedness. This article describes the identification procedures and tools used and presents a profile of the children in terms of socioeconomic, intellectual, and personality variables. (Author/JDD)

  2. Enhancing lineup identification accuracy: two codes are better than one.

    PubMed

    Melara, R D; DeWitt-Rickards, T S; O'Brien, T P

    1989-10-01

    Ways of improving identification accuracy were explored by comparing the conventional visual lineup with an auditory/visual lineup, one that paired color photographs with voice recordings. This bimodal lineup necessitated sequential presentation of lineup members; Experiment 1 showed that performance in sequential lineups was better than performance in traditional simultaneous lineups. In Experiments 2A and 2B unimodal and bimodal lineups were compared by using a multiple-lineup paradigm: Ss viewed 3 videotaped episodes depicting standard police procedures and were tested in 4 sequential lineups. Bimodal lineups were more diagnostic than either visual or auditory lineups alone. The bimodal lineup led to a 126% improvement in number of correct identifications over the conventional visual lineup, with no concomitant increase in number of false identifications. These results imply strongly that bimodal procedures should be adopted in real-world lineups. The nature of memorial processes underlying this bimodal advantage is discussed.

  3. Attitude determination and calibration using a recursive maximum likelihood-based adaptive Kalman filter

    NASA Technical Reports Server (NTRS)

    Kelly, D. A.; Fermelia, A.; Lee, G. K. F.

    1990-01-01

    An adaptive Kalman filter design that utilizes recursive maximum likelihood parameter identification is discussed. At the center of this design is the Kalman filter itself, which has the responsibility for attitude determination. At the same time, the identification algorithm is continually identifying the system parameters. The approach is applicable to nonlinear, as well as linear systems. This adaptive Kalman filter design has much potential for real time implementation, especially considering the fast clock speeds, cache memory and internal RAM available today. The recursive maximum likelihood algorithm is discussed in detail, with special attention directed towards its unique matrix formulation. The procedure for using the algorithm is described along with comments on how this algorithm interacts with the Kalman filter.

  4. Rapid Detection and Identification of Candidemia by Direct Blood Culturing on Solid Medium by Use of Lysis-Centrifugation Method Combined with Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry (MALDI-TOF MS).

    PubMed

    Idelevich, Evgeny A; Grünastel, Barbara; Becker, Karsten

    2017-01-01

    Candida sepsis is a life-threatening condition with increasing prevalence. In this study, direct blood culturing on solid medium using a lysis-centrifugation procedure enabled successful Candida species identification by matrix-assisted laser desorption-ionization time of flight mass spectrometry on average 3.8 h (Sabouraud agar) or 7.4 h (chocolate agar) before the positivity signal for control samples in Bactec mycosis-IC/F or Bactec Plus aerobic/F bottles, respectively. Direct culturing on solid medium accelerated candidemia diagnostics compared to that with automated broth-based systems. Copyright © 2016 American Society for Microbiology.

  5. Risk analysis procedure for post-wildfire natural hazards in British Columbia

    NASA Astrophysics Data System (ADS)

    Jordan, Peter

    2010-05-01

    Following a severe wildfire season in 2003, and several subsequent damaging debris flow and flood events, the British Columbia Forest Service developed a procedure for analysing risks to public safety and infrastructure from such events. At the same time, the Forest Service undertook a research program to determine the extent of post-wildfire hazards, and examine the hydrologic and geomorphic processes contributing to the hazards. The risk analysis procedure follows the Canadian Standards Association decision-making framework for risk management (which in turn is based on international standards). This has several steps: identification of risk, risk analysis and estimation, evaluation of risk tolerability, developing control or mitigation strategies, and acting on these strategies. The Forest Service procedure deals only with the first two steps. The results are passed on to authorities such as the Provincial Emergency Program and local government, who are responsible for evaluating risks, warning residents, and applying mitigation strategies if appropriate. The objective of the procedure is to identify and analyse risks to public safety and infrastructure. The procedure is loosely based on the BAER (burned area emergency response) program in the USA, with some important differences. Our procedure focuses on identifying risks and warning affected parties, not on mitigation activities such as broadcast erosion control measures. Partly this is due to limited staff and financial resources. Also, our procedure is not multi-agency, but is limited to wildfires on provincial forest land; in British Columbia about 95% of forest land is in the publicly-owned provincial forest. Each fire season, wildfires are screened by size and proximity to values at risk such as populated areas. For selected fires, when the fire is largely contained, the procedure begins with an aerial reconnaissance of the fire, and photography with a hand-held camera, which can be used to make a preliminary map of vegetation burn severity if desired. The next steps include mapping catchment boundaries, field traverses to collect data on soil burn severity and water repellency, identification of unstable hillslopes and channels, and inspection of values at risk from hazards such as debris flows or flooding. BARC (burned area reflectance classification) maps based on satellite imagery are prepared for some fires, although these are typically not available for several weeks. Our objective is to make a preliminary risk analysis report available about two weeks after the fire is contained. If high risks to public safety or infrastructure are identified, the risk analysis reports may make recommendations for mitigation measures to be considered; however, acting on these recommendations is the responsibility of local land managers, local government, or landowners. Mitigation measures for some fires have included engineering treatments to reduce the hydrologic impact of logging roads, protective structures such as dykes or berms, and straw mulching to reduce runoff and erosion on severely burned areas. The Terrace Mountain Fire, with burned 9000 hectares in the Okanagan Valley in 2009, is used as an example of the application of the procedure.

  6. Research on Intelligent Interface in Double-front Work Machines

    NASA Astrophysics Data System (ADS)

    Kamezaki, Mitsuhiro; Iwata, Hiroyasu; Sugano, Shigeki

    This paper proposes a work state identification method with full independent of work environmental conditions and operator skill levels for construction machinery. Advanced operated-work machines, which have been designed for complicated tasks, require intelligent systems that can provide the quantitative work analysis needed to determine effective work procedures and that can provide operational and cognitive support for operators. Construction work environments are extremely complicated, however, and this makes state identification, which is a key technology for an intelligent system, difficult. We therefore defined primitive static states (PSS) that are determined using on-off information for the lever inputs and manipulator loads for each part of the grapple and front and that are completely independent of the various environmental conditions and variation in operator skill level that can cause an incorrect work state identification. To confirm the usefulness of PSS, we performed experiments with a demolition task by using our virtual reality simulator. We confirmed that PSS could robustly and accurately identify the work states and that untrained skills could be easily inferred from the results of PSS-based work analysis. We also confirmed in skill-training experiments that advice information based on PSS-based skill analysis greatly improved operator's work performance. We thus confirmed that PSS can adequately identify work states and are useful for work analysis and skill improvement.

  7. 6 CFR 5.45 - Procedure when testimony or production of documents is sought; general.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 6 Domestic Security 1 2010-01-01 2010-01-01 false Procedure when testimony or production of... Procedure when testimony or production of documents is sought; general. (a) If official information is... requiring oral testimony, and identification of potentially relevant documents. (c) The appropriate...

  8. A description of the reformatted spring small grains labeling procedure used in test 2, part 2 of the US/Canada wheat and barley exploratory experiment

    NASA Technical Reports Server (NTRS)

    Palmer, W. F.; Magness, E. R. (Principal Investigator)

    1981-01-01

    The reformatted spring small grains labeling procedure is designed to be used for assigning crop identification labels to a predetermined and selected number of dots. The development and description of this procedure is presented.

  9. Need Assessment: Winnowing Expressed Concerns for Critical Needs. A Training Manual.

    ERIC Educational Resources Information Center

    Eastmond, Jefferson N.

    A procedure is given for determining various concerns for the identification of priority needs or important problems. Chapters deal with procedures for harvesting educational concerns, illustrations of the systematic harvesting of concerns, concerns classification and analysis, and conducting the need assessment. A diagram of the procedure for…

  10. 30 CFR 250.917 - What are the CVA's primary duties during the fabrication phase?

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... adherence to such procedures; (v) Welder and welding procedure qualification and identification; (vi... requirements, and evaluation results of the specified examinations; (viii) Destructive testing requirements and...

  11. Procedural justice, occupational identification, and organizational commitment.

    DOT National Transportation Integrated Search

    1992-06-01

    Extending Tyler's (1989) group-value model, the present study tested the hypothesis that procedural justice may be of differential salience in the development of organizational commitment among individuals who identify primarily with their employing ...

  12. Nonparametric identification of nonlinear dynamic systems using a synchronisation-based method

    NASA Astrophysics Data System (ADS)

    Kenderi, Gábor; Fidlin, Alexander

    2014-12-01

    The present study proposes an identification method for highly nonlinear mechanical systems that does not require a priori knowledge of the underlying nonlinearities to reconstruct arbitrary restoring force surfaces between degrees of freedom. This approach is based on the master-slave synchronisation between a dynamic model of the system as the slave and the real system as the master using measurements of the latter. As the model synchronises to the measurements, it becomes an observer of the real system. The optimal observer algorithm in a least-squares sense is given by the Kalman filter. Using the well-known state augmentation technique, the Kalman filter can be turned into a dual state and parameter estimator to identify parameters of a priori characterised nonlinearities. The paper proposes an extension of this technique towards nonparametric identification. A general system model is introduced by describing the restoring forces as bilateral spring-dampers with time-variant coefficients, which are estimated as augmented states. The estimation procedure is followed by an a posteriori statistical analysis to reconstruct noise-free restoring force characteristics using the estimated states and their estimated variances. Observability is provided using only one measured mechanical quantity per degree of freedom, which makes this approach less demanding in the number of necessary measurement signals compared with truly nonparametric solutions, which typically require displacement, velocity and acceleration signals. Additionally, due to the statistical rigour of the procedure, it successfully addresses signals corrupted by significant measurement noise. In the present paper, the method is described in detail, which is followed by numerical examples of one degree of freedom (1DoF) and 2DoF mechanical systems with strong nonlinearities of vibro-impact type to demonstrate the effectiveness of the proposed technique.

  13. Genetically encoded releasable photo-cross-linking strategies for studying protein-protein interactions in living cells.

    PubMed

    Yang, Yi; Song, Haiping; He, Dan; Zhang, Shuai; Dai, Shizhong; Xie, Xiao; Lin, Shixian; Hao, Ziyang; Zheng, Huangtao; Chen, Peng R

    2017-10-01

    Although protein-protein interactions (PPIs) have crucial roles in virtually all cellular processes, the identification of more transient interactions in their biological context remains challenging. Conventional photo-cross-linking strategies can be used to identify transient interactions, but these approaches often suffer from high background due to the cross-linked bait proteins. To solve the problem, we have developed membrane-permeable releasable photo-cross-linkers that allow for prey-bait separation after protein complex isolation and can be installed in proteins of interest (POIs) as unnatural amino acids. Here we describe the procedures for using two releasable photo-cross-linkers, DiZSeK and DiZHSeC, in both living Escherichia coli and mammalian cells. A cleavage after protein photo-cross-linking (CAPP ) strategy based on the photo-cross-linker DiZSeK is described, in which the prey protein pool is released from a POI after affinity purification. Prey proteins are analyzed using mass spectrometry or 2D gel electrophoresis for global comparison of interactomes from different experimental conditions. An in situ cleavage and mass spectrometry (MS)-label transfer after protein photo-cross-linking (IMAPP) strategy based on the photo-cross-linker DiZHSeC is also described. This strategy can be used for the identification of cross-linking sites to allow detailed characterization of PPI interfaces. The procedures for photo-cross-linker incorporation, photo-cross-linking of interaction partners and affinity purification of cross-linked complexes are similar for the two photo-cross-linkers. The final section of the protocol describes prey-bait separation (for CAPP) and MS-label transfer and identification (for IMAPP). After plasmid construction, the CAPP and IMAPP strategies can be completed within 6 and 7 d, respectively.

  14. The use of 2-dodecylcyclobutanone for the identification of irradiated chicken meat and eggs

    NASA Astrophysics Data System (ADS)

    Stevenson, M. H.; Crone, A. V. J.; Hamilton, J. T. G.; McMurray, C. H.

    1993-07-01

    A procedure has been developed for the detection of 2-alkylcyclobutanones which are useful markers for the identification of irradiated chicken meat and liquid whole egg. The compounds appear to be specific for irradiation since they are not generated by cooking, packing in vacuum or CO 2 and are sufficiently stable to be detected throughout the shelf-life of these products. As the irradiation dose increases there is a linear increase in the amount of these compounds formed in chicken meat and so the method has potential for the estimation of irradiation dose. The procedure developed should be applicable for the identification of a range of foods of varying fat and fatty acid composition.

  15. Identification of Carboxypeptidase Substrates by C-Terminal COFRADIC.

    PubMed

    Tanco, Sebastian; Aviles, Francesc Xavier; Gevaert, Kris; Lorenzo, Julia; Van Damme, Petra

    2017-01-01

    We here present a detailed procedure for studying protein C-termini and their posttranslational modifications by C-terminal COFRADIC. In fact, this procedure can enrich for both C-terminal and N-terminal peptides through a combination of a strong cation exchange fractionation step at low pH, which removes the majority of nonterminal peptides in whole-proteome digests, while the actual COFRADIC step segregates C-terminal peptides from N-terminal peptides. When used in a differential mode, C-terminal COFRADIC allows for the identification of neo-C-termini generated by the action of proteases, which in turn leads to the identification of protease substrates. More specifically, this technology can be applied to determine the natural substrate repertoire of carboxypeptidases on a proteome-wide scale.

  16. Robust uncertainty evaluation for system identification on distributed wireless platforms

    NASA Astrophysics Data System (ADS)

    Crinière, Antoine; Döhler, Michael; Le Cam, Vincent; Mevel, Laurent

    2016-04-01

    Health monitoring of civil structures by system identification procedures from automatic control is now accepted as a valid approach. These methods provide frequencies and modeshapes from the structure over time. For a continuous monitoring the excitation of a structure is usually ambient, thus unknown and assumed to be noise. Hence, all estimates from the vibration measurements are realizations of random variables with inherent uncertainty due to (unknown) process and measurement noise and finite data length. The underlying algorithms are usually running under Matlab under the assumption of large memory pool and considerable computational power. Even under these premises, computational and memory usage are heavy and not realistic for being embedded in on-site sensor platforms such as the PEGASE platform. Moreover, the current push for distributed wireless systems calls for algorithmic adaptation for lowering data exchanges and maximizing local processing. Finally, the recent breakthrough in system identification allows us to process both frequency information and its related uncertainty together from one and only one data sequence, at the expense of computational and memory explosion that require even more careful attention than before. The current approach will focus on presenting a system identification procedure called multi-setup subspace identification that allows to process both frequencies and their related variances from a set of interconnected wireless systems with all computation running locally within the limited memory pool of each system before being merged on a host supervisor. Careful attention will be given to data exchanges and I/O satisfying OGC standards, as well as minimizing memory footprints and maximizing computational efficiency. Those systems are built in a way of autonomous operations on field and could be later included in a wide distributed architecture such as the Cloud2SM project. The usefulness of these strategies is illustrated on data from a progressive damage action on a prestressed concrete bridge. References [1] E. Carden and P. Fanning. Vibration based condition monitoring: a review. Structural Health Monitoring, 3(4):355-377, 2004. [2] M. Döhler and L. Mevel. Efficient multi-order uncertainty computation for stochastic subspace identification. Mechanical Systems and Signal Processing, 38(2):346-366, 2013. [3] M.Döhler, L. Mevel. Modular subspace-based system identification from multi-setup measurements. IEEE Transactions on Automatic Control, 57(11):2951-2956, 2012. [4] M. Döhler, X.-B. Lam, and L. Mevel. Uncertainty quantification for modal parameters from stochastic subspace identification on multi-setup measurements. MechanicalSystems and Signal Processing, 36(2):562-581, 2013. [5] A Crinière, J Dumoulin, L Mevel, G Andrade-Barosso, M Simonin. The Cloud2SM Project.European Geosciences Union General Assembly (EGU2015), Apr 2015, Vienne, Austria. 2015.

  17. Variation in Microbial Identification System Accuracy for Yeast Identification Depending on Commercial Source of Sabouraud Dextrose Agar

    PubMed Central

    Kellogg, James A.; Bankert, David A.; Chaturvedi, Vishnu

    1999-01-01

    The accuracy of the Microbial Identification System (MIS; MIDI, Inc.) for identification of yeasts to the species level was compared by using 438 isolates grown on prepoured BBL Sabouraud dextrose agar (SDA) and prepoured Remel SDA. Correct identification was observed for 326 (74%) of the yeasts cultured on BBL SDA versus only 214 (49%) of yeasts grown on Remel SDA (P < 0.001). The commercial source of the SDA used in the MIS procedure significantly influences the system’s accuracy. PMID:10325387

  18. Early Identification of Emotionally Handicapped Children in School, Second Edition. A Monograph in American Lectures in Psychology.

    ERIC Educational Resources Information Center

    Bower, Eli M.

    Directed to teachers and other school personnel, the text presents screening procedures to aid in the early identification of the emotionally handicapped. The nature of preventive mental health, emotional handicap, and problems in early identification are considered; also considered are the direction and trends of past research. A study in early…

  19. [Assessment of the implementation of an unambiguous patient identification system in an acute care hospital].

    PubMed

    Martínez-Ochoa, Eva M; Cestafe-Martínez, Adolfo; Martínez-Sáenz, M Soledad; Belío-Blasco, Cristina; Caro-Berguilla, Yolanda; Rivera-Sanz, Félix

    2010-07-01

    To achieve implantation of unequivocal identification of all admitted patients, to ensure the identification of patients with an individual bracelet integrated into the clinical record, and to involve health professionals in this process. A working group was created, which analyzed the current situation in the hospital, selected materials, and designed the patient identification procedure and support material for patients and health professionals. After the system was implemented, coverage was assessed through direct observation. Implementation and satisfaction among patients and health professionals was evaluated through specifically designed questionnaires. Coverage was 79.4%. Most (82.8%) professionals knew why the identification bracelet was used and 57.8% thought it helped to avoid patient identification errors. Twenty percent used the bracelet data when administering medication, 29.2% when taking blood samples and 25.6% on entry to the operating room. Nearly all (88.3%) patients reported that the bracelet was not uncomfortable and 62.8% reported they received no information when the bracelet was placed. Acceptable coverage of the patient identification bracelets was achieved. However, the involvement of health professionals in the identification process was low, since the bracelets were not routinely used in established procedures and patients were only infrequently provided with information when the bracelets were placed. Copyright © 2010 Elsevier España S.L. All rights reserved.

  20. Where are we coming from versus who we will become: the effect of priming different contents of European identity on cooperation.

    PubMed

    La Barbera, Francesco; Ferrara, Pia Cariota; Boza, Mihaela

    2014-12-01

    In two experiments, we investigated how priming European identity as common project versus common heritage affects participants' cooperation in a social dilemma; an additional aim was to explore the mediators involved in the process. In the first experiment, 82 students played a public good dilemma with a European bogus partner and then completed self-report measures of identification with the European Union (EU), group-based trust and collective interest. Results showed that priming a common project-based but not a common heritage-based European social identity fostered cooperative behaviour; this effect was mediated by two sequential mediators: the common project prime increased participants' strength of identification with EU (mediator 1) which, in turn, positively affected group-based trust (mediator 2), fostering greater cooperation. Experiment 2 was conducted with a similar procedure on a sample of 124 students, using a different measure of trust and changing the order of mediators. Results supported those of previous experiment: Priming a project-based EU identity content (compared to heritage-based one) had significant direct and indirect effects on cooperation. © 2014 International Union of Psychological Science.

  1. The use of UV, FT-IR and Raman spectra for the identification of the newest penem analogs: solutions based on mathematic procedure and the density functional theory.

    PubMed

    Cielecka-Piontek, J; Lewandowska, K; Barszcz, B; Paczkowska, M

    2013-02-15

    The application of ultraviolet, FT-IR and Raman spectra was proposed for identification studies of the newest penem analogs (doripenem, biapenem and faropenem). An identification of the newest penem analogs based on their separation from related substances was achieved after the application of first derivative of direct spectra in ultraviolet which permitted elimination of overlapping effects. A combination of experimental and theoretical studies was performed for analyzing the structure and vibrational spectra (FT-IR and Raman spectra) of doripenem, biapenem and faropenem. The calculations were conducted using the density functional theory with the B3LYP hybrid functional and 6-31G(d,p) basis set. The confirmation of the applicability of the DFT methodology for interpretation of vibrational IR and Raman spectra of the newest penem analogs contributed to determination of changes of vibrations in the area of the most labile bonds. By employing the theoretical approach it was possible to eliminate necessity of using reference standards which - considering the instability of penem analogs - require that correction coefficients are factored in. Copyright © 2012 Elsevier B.V. All rights reserved.

  2. Spectrometric microbiological analyzer

    NASA Astrophysics Data System (ADS)

    Schlager, Kenneth J.; Meissner, Ken E.

    1996-04-01

    Currently, there are four general approaches to microbiological analysis, i.e., the detection, identification and quantification of micro-organisms: (1) Traditional culturing and staining procedures, metabolic fermentations and visual morphological characteristics; (2) Immunological approaches employing microbe-specific antibodies; (3) Biotechnical techniques employing DNA probes and related genetic engineering methods; and (4) Physical measurement techniques based on the biophysical properties of micro-organisms. This paper describes an instrumentation development in the fourth of the above categories, physical measurement, that uses a combination of fluorometric and light scatter spectra to detect and identify micro-organisms at the species level. A major advantage of this approach is the rapid turnaround possible in medical diagnostic or water testing applications. Fluorometric spectra serve to define the biochemical characteristics of the microbe, and light scatter spectra the size and shape morphology. Together, the two spectra define a 'fingerprint' for each species of microbe for detection, identification and quantification purposes. A prototype instrument has been developed and tested under NASA sponsorship based on fluorometric spectra alone. This instrument demonstrated identification and quantification capabilities at the species level. The paper reports on test results using this instrument, and the benefits of employing a combination of fluorometric and light scatter spectra.

  3. A reference estimator based on composite sensor pattern noise for source device identification

    NASA Astrophysics Data System (ADS)

    Li, Ruizhe; Li, Chang-Tsun; Guan, Yu

    2014-02-01

    It has been proved that Sensor Pattern Noise (SPN) can serve as an imaging device fingerprint for source camera identification. Reference SPN estimation is a very important procedure within the framework of this application. Most previous works built reference SPN by averaging the SPNs extracted from 50 images of blue sky. However, this method can be problematic. Firstly, in practice we may face the problem of source camera identification in the absence of the imaging cameras and reference SPNs, which means only natural images with scene details are available for reference SPN estimation rather than blue sky images. It is challenging because the reference SPN can be severely contaminated by image content. Secondly, the number of available reference images sometimes is too few for existing methods to estimate a reliable reference SPN. In fact, existing methods lack consideration of the number of available reference images as they were designed for the datasets with abundant images to estimate the reference SPN. In order to deal with the aforementioned problem, in this work, a novel reference estimator is proposed. Experimental results show that our proposed method achieves better performance than the methods based on the averaged reference SPN, especially when few reference images used.

  4. Using web-based observations to identify thresholds of a person's stability in a flow

    NASA Astrophysics Data System (ADS)

    Milanesi, L.; Pilotti, M.; Bacchi, B.

    2016-10-01

    Flood risk assessment and mitigation are important tasks that should take advantage of rational vulnerability models to increase their effectiveness. These models are usually identified through a relevant set of laboratory experiments. However, there is growing evidence that these tests are not fully representative of the variety of conditions that characterize real flood hazard situations. This paper suggests a citizen science-based and innovative approach to obtain information from web resources for the calibration of people's vulnerability models. A comprehensive study employing commonly used web engines allowed the collection of a wide set of documents showing real risk situations for people impacted by floods, classified according to the stability of the involved subjects. A procedure to extrapolate the flow depth and velocity from the video frames is developed and its reliability is verified by comparing the results with observation. The procedure is based on the statistical distribution of the population height employing a direct uncertainty propagation method. The results complement the experimental literature data and conceptual models. The growing availability of online information will progressively increase the sample size on which the procedure is based and will eventually lead to the identification of a probability surface describing the transition between stability and instability conditions of individuals in a flow.

  5. Quantitative non-invasive intracellular imaging of Plasmodium falciparum infected human erythrocytes

    NASA Astrophysics Data System (ADS)

    Edward, Kert; Farahi, Faramarz

    2014-05-01

    Malaria is a virulent pathological condition which results in over a million annual deaths. The parasitic agent Plasmodium falciparum has been extensively studied in connection with this epidemic but much remains unknown about its development inside the red blood cell host. Optical and fluorescence imaging are among the two most common procedures for investigating infected erythrocytes but both require the introduction of exogenous contrast agents. In this letter, we present a procedure for the non-invasive in situ imaging of malaria infected red blood cells. The procedure is based on the utilization of simultaneously acquired quantitative phase and independent topography data to extract intracellular information. Our method allows for the identification of the developmental stages of the parasite and facilitates in situ analysis of the morphological changes associated with the progression of this disease. This information may assist in the development of efficacious treatment therapies for this condition.

  6. Articulated Arm Coordinate Measuring Machine Calibration by Laser Tracker Multilateration

    PubMed Central

    Majarena, Ana C.; Brau, Agustín; Velázquez, Jesús

    2014-01-01

    A new procedure for the calibration of an articulated arm coordinate measuring machine (AACMM) is presented in this paper. First, a self-calibration algorithm of four laser trackers (LTs) is developed. The spatial localization of a retroreflector target, placed in different positions within the workspace, is determined by means of a geometric multilateration system constructed from the four LTs. Next, a nonlinear optimization algorithm for the identification procedure of the AACMM is explained. An objective function based on Euclidean distances and standard deviations is developed. This function is obtained from the captured nominal data (given by the LTs used as a gauge instrument) and the data obtained by the AACMM and compares the measured and calculated coordinates of the target to obtain the identified model parameters that minimize this difference. Finally, results show that the procedure presented, using the measurements of the LTs as a gauge instrument, is very effective by improving the AACMM precision. PMID:24688418

  7. 20 CFR 422.112 - Employer identification numbers.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 20 Employees' Benefits 2 2010-04-01 2010-04-01 false Employer identification numbers. 422.112 Section 422.112 Employees' Benefits SOCIAL SECURITY ADMINISTRATION ORGANIZATION AND PROCEDURES General..., and domestic corporations which elect social security coverage for employees of foreign subsidiaries...

  8. Best Practices in Intellectual Disability Identification

    ERIC Educational Resources Information Center

    Fiorello, Catherine A.; Jenkins, Tiffany K.

    2018-01-01

    This article is an overview of identification of intellectual disabilities (ID), with a focus on meeting legal and ethical requirements when assessing children from culturally and linguistically diverse backgrounds and those living in poverty. Specific procedures and recommended instruments will be reviewed.

  9. Identification of exhumed remains of fire tragedy victims using conventional methods and autosomal/Y-chromosomal short tandem repeat DNA profiling.

    PubMed

    Calacal, Gayvelline C; Delfin, Frederick C; Tan, Michelle Music M; Roewer, Lutz; Magtanong, Danilo L; Lara, Myra C; Fortun, Raquel dR; De Ungria, Maria Corazon A

    2005-09-01

    In a fire tragedy in Manila in December 1998, one of the worst tragic incidents which resulted in the reported death of 23 children, identity could not be established initially resulting in the burial of still unidentified bodies. Underscoring the importance of identifying each of the human remains, the bodies were exhumed 3 months after the tragedy. We describe here our work, which was the first national case handled by local laboratories wherein conventional and molecular-based techniques were successfully applied in forensic identification. The study reports analysis of DNA obtained from skeletal remains exposed to conditions of burning, burial, and exhumation. DNA typing methods using autosomal and Y-chromosomal short tandem repeat (Y-STR) markers reinforced postmortem examinations using conventional identification techniques. The strategy resulted in the identification of 18 out of the 21 human remains analyzed, overcoming challenges encountered due to the absence of established procedures for the recovery of mass disaster remains. There was incomplete antemortem information to match the postmortem data obtained from the remains of 3 female child victims. Two victims were readily identified due to the availability of antemortem tissues. In the absence of this biologic material, parentage testing was performed using reference blood samples collected from parents and relatives. Data on patrilineal lineage based on common Y-STR haplotypes augmented autosomal DNA typing, particularly in deficiency cases.

  10. Echodentography based on nonlinear time reversal tomography: Ultrasonic nonlinear signature identification

    NASA Astrophysics Data System (ADS)

    Santos, Serge Dos; Farova, Zuzana; Kus, Vaclav; Prevorovsky, Zdenek

    2012-05-01

    This paper examines possibilities of using Nonlinear Elastic Wave Spectroscopy (NEWS) methods in dental investigations. Themain task consisted in imaging cracks or other degradation signatures located in dentin close to the Enamel-Dentine Junction (EDJ). NEWS approach was investigated experimentally with a new bi-modal acousto-optic set-up based on the chirp-coded nonlinear ultrasonic time reversal (TR) concepts. Complex internal structure of the tooth is analyzed by the TR-NEWS procedure adapted to tomography-like imaging of the tooth damages. Ultrasonic instrumentation with 10 MHz bandwidth has been set together including laser vibrometer used to detect responses of the tooth on its excitation carried out by a contact piezoelectric transducer. Bi-modal TR-NEWS images of the tooth were created before and after focusing, which resulted from the time compression. The polar B-scan of the tooth realized with TR-NEWS procedure is suggested to be applied as a new echodentography imaging.

  11. Dynamic metabolic modeling for a MAB bioprocess.

    PubMed

    Gao, Jianying; Gorenflo, Volker M; Scharer, Jeno M; Budman, Hector M

    2007-01-01

    Production of monoclonal antibodies (MAb) for diagnostic or therapeutic applications has become an important task in the pharmaceutical industry. The efficiency of high-density reactor systems can be potentially increased by model-based design and control strategies. Therefore, a reliable kinetic model for cell metabolism is required. A systematic procedure based on metabolic modeling is used to model nutrient uptake and key product formation in a MAb bioprocess during both the growth and post-growth phases. The approach combines the key advantages of stoichiometric and kinetic models into a complete metabolic network while integrating the regulation and control of cellular activity. This modeling procedure can be easily applied to any cell line during both the cell growth and post-growth phases. Quadratic programming (QP) has been identified as a suitable method to solve the underdetermined constrained problem related to model parameter identification. The approach is illustrated for the case of murine hybridoma cells cultivated in stirred spinners.

  12. Remote sensing based on hyperspectral data analysis

    NASA Astrophysics Data System (ADS)

    Sharifahmadian, Ershad

    In remote sensing, accurate identification of far objects, especially concealed objects is difficult. In this study, to improve object detection from a distance, the hyperspecral imaging and wideband technology are employed with the emphasis on wideband radar. As the wideband data includes a broad range of frequencies, it can reveal information about both the surface of the object and its content. Two main contributions are made in this study: 1) Developing concept of return loss for target detection: Unlike typical radar detection methods which uses radar cross section to detect an object, it is possible to enhance the process of detection and identification of concealed targets using the wideband radar based on the electromagnetic characteristics --conductivity, permeability, permittivity, and return loss-- of materials. During the identification process, collected wideband data is evaluated with information from wideband signature library which has already been built. In fact, several classes (e.g. metal, wood, etc.) and subclasses (ex. metals with high conductivity) have been defined based on their electromagnetic characteristics. Materials in a scene are then classified based on these classes. As an example, materials with high electrical conductivity can be conveniently detected. In fact, increasing relative conductivity leads to a reduction in the return loss. Therefore, metals with high conductivity (ex. copper) shows stronger radar reflections compared with metals with low conductivity (ex. stainless steel). Thus, it is possible to appropriately discriminate copper from stainless steel. 2) Target recognition techniques: To detect and identify targets, several techniques have been proposed, in particular the Multi-Spectral Wideband Radar Image (MSWRI) which is able to localize and identify concealed targets. The MSWRI is based on the theory of robust capon beamformer. During identification process, information from wideband signature library is utilized. The WB signature library includes such parameters as conductivity, permeability, permittivity, and return loss at different frequencies for possible materials related to a target. In the MSWRI approach, identification procedure is performed by calculating the RLs at different selected frequencies. Based on similarity of the calculated RLs and RL from WB signature library, targets are detected and identified. Based on the simulation and experimental results, it is concluded that the MSWRI technique is a promising approach for standoff target detection.

  13. 42 CFR 420.304 - Procedures for obtaining access to books, documents, and records.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 42 Public Health 3 2010-10-01 2010-10-01 false Procedures for obtaining access to books, documents... Books, Documents, and Records of Subcontractors § 420.304 Procedures for obtaining access to books... following elements: (1) Reasonable identification of the books, documents, and records to which access is...

  14. Identification of inferior pancreaticoduodenal artery during pancreaticoduodenectomy using augmented reality-based navigation system.

    PubMed

    Onda, Shinji; Okamoto, Tomoyoshi; Kanehira, Masaru; Suzuki, Fumitake; Ito, Ryusuke; Fujioka, Shuichi; Suzuki, Naoki; Hattori, Asaki; Yanaga, Katsuhiko

    2014-04-01

    In pancreaticoduodenectomy (PD), early ligation of the inferior pancreaticoduodenal artery (IPDA) before efferent veins has been advocated to decrease blood loss by congestion of the pancreatic head to be resected. In this study, we herein report the utility of early identification of the IPDA using an augmented reality (AR)-based navigation system (NS). Seven nonconsecutive patients underwent PD using AR-based NS. After paired-point matching registration, the reconstructed image obtained by preoperative computed tomography (CT) was fused with a real-time operative field image and displayed on 3D monitors. The vascular reconstructed images, including the superior mesenteric artery, jejunal artery, and IPDA were visualized to facilitate image-guided surgical procedures. We compared operating time and intraoperative blood loss of six patients who successfully underwent identification of IPDA using AR-based NS (group A) with nine patients who underwent early ligation of IPDA without using AR (group B) and 18 patients who underwent a conventional PD (group C). The IPDA or the jejunal artery was rapidly identified and ligated in six patients. The mean operating time and intraoperative blood loss in group A was 415 min and 901 ml, respectively. There was no significant difference in operating time and intraoperative blood loss among the groups. The AR-based NS provided precise anatomical information, which allowed the surgeons to rapidly identify and perform early ligation of IPDA in PD. © 2013 Japanese Society of Hepato-Biliary-Pancreatic Surgery.

  15. Selective Formation of Secondary Amides via the Copper-Catalyzed Cross-Coupling of Alkylboronic Acids with Primary Amides

    PubMed Central

    Rossi, Steven A.; Shimkin, Kirk W.; Xu, Qun; Mori-Quiroz, Luis M.; Watson, Donald A.

    2014-01-01

    For the first time, a general catalytic procedure for the cross coupling of primary amides and alkylboronic acids is demonstrated. The key to the success of this reaction was the identification of a mild base (NaOSiMe3) and oxidant (di-tert-butyl peroxide) to promote the copper-catalyzed reaction in high yield. This transformation provides a facile, high-yielding method for the mono-alkylation of amides. PMID:23611591

  16. A novel surgical management of hypopharyngeal branchial anomalies.

    PubMed

    Givens, Daniel J; Buchmann, Luke O; Park, Albert H

    2015-04-01

    To review our experience treating hypopharyngeal branchial anomalies utilizing an open transcervical approach that: (1) includes recurrent laryngeal nerve (RLN) monitoring and identification if needed; (2) resection of tract if present; and (3) a superiorly based sternothyroid muscle flap for closure. A retrospective chart review was performed to identify all patients at a tertiary level children's hospital with branchial anomalies from 2005 to 2014. The clinical presentation, evaluation, treatment and outcome were analyzed for those patients with hypopharyngeal branchial anomalies. Forty-seven patients who underwent excision of branchial anomalies with a known origin were identified. Thirteen patients had hypopharyngeal branchial anomalies. Six of these patients were treated by the authors of this study and are the focus of this analysis. All six underwent an open transcervical procedure with a sternothyroid muscle flap closure of a piriform sinus opening over a nine year period. Definitive surgery included a microlaryngoscopy and an open transcervical approach to close a fistula between the piriform sinus and neck with recurrent laryngeal nerve monitoring or dissection. A superiorly based sternothyroid muscle flap was used to close the sinus opening. There were no recurrences, recurrent laryngeal nerve injuries or other complications from these procedures. This study supports complete surgical extirpation of the fistula tract using an open cervical approach, recurrent laryngeal nerve monitoring or identification, and rotational muscle flap closure to treat patients with hypopharyngeal branchial anomalies. Published by Elsevier Ireland Ltd.

  17. Analysis of archaeological triacylglycerols by high resolution nanoESI, FT-ICR MS and IRMPD MS/MS: Application to 5th century BC-4th century AD oil lamps from Olbia (Ukraine)

    NASA Astrophysics Data System (ADS)

    Garnier, Nicolas; Rolando, Christian; Høtje, Jakob Munk; Tokarski, Caroline

    2009-07-01

    This work presents the precise identification of triacylglycerols (TAGs) extracted from archaeological samples using a methodology based on nanoelectrospray and Fourier transform mass spectrometry. The archaeological TAG identification needs adapted sample preparation protocols to trace samples in advanced degradation state. More precisely, the proposed preparation procedure includes extraction of the lipid components from finely grinded ceramic using dichloromethane/methanol mixture with additional ultrasonication treatment, and TAG purification by solid phase extraction on a diol cartridge. Focusing on the analytical approach, the implementation of "in-house" species-dependent TAG database was investigated using MS and InfraRed Multiphoton Dissociation (IRMPD) MS/MS spectra; several vegetal oils, dairy products and animal fats were studied. The high mass accuracy of the Fourier transform analyzer ([Delta]m below 2.5 ppm) provides easier data interpretation, and allows distinction between products of different origins. In details, the IRMPD spectra of the lithiated TAGs reveal fragmentation reactions including loss of free neutral fatty acid and loss of fatty acid as [alpha],[beta]-unsaturated moieties. Based on the developed preparation procedure and on the constituted database, TAG extracts from 5th century BC to 4th century AD Olbia lamps were analyzed. The structural information obtained succeeds in identifying that bovine/ovine fats were used as fuel used in these archaeological Olbia lamps.

  18. A Novel Approach for Adaptive Signal Processing

    NASA Technical Reports Server (NTRS)

    Chen, Ya-Chin; Juang, Jer-Nan

    1998-01-01

    Adaptive linear predictors have been used extensively in practice in a wide variety of forms. In the main, their theoretical development is based upon the assumption of stationarity of the signals involved, particularly with respect to the second order statistics. On this basis, the well-known normal equations can be formulated. If high- order statistical stationarity is assumed, then the equivalent normal equations involve high-order signal moments. In either case, the cross moments (second or higher) are needed. This renders the adaptive prediction procedure non-blind. A novel procedure for blind adaptive prediction has been proposed and considerable implementation has been made in our contributions in the past year. The approach is based upon a suitable interpretation of blind equalization methods that satisfy the constant modulus property and offers significant deviations from the standard prediction methods. These blind adaptive algorithms are derived by formulating Lagrange equivalents from mechanisms of constrained optimization. In this report, other new update algorithms are derived from the fundamental concepts of advanced system identification to carry out the proposed blind adaptive prediction. The results of the work can be extended to a number of control-related problems, such as disturbance identification. The basic principles are outlined in this report and differences from other existing methods are discussed. The applications implemented are speech processing, such as coding and synthesis. Simulations are included to verify the novel modelling method.

  19. A new approach to the identification of Landscape Quality Objectives (LQOs) as a set of indicators.

    PubMed

    Sowińska-Świerkosz, Barbara Natalia; Chmielewski, Tadeusz J

    2016-12-15

    The objective of the paper is threefold: (1) to introduce Landscape Quality Objectives (LQOs) as a set of indicators; (2) to present a method of linking social and expert opinion in the process of the formulation of landscape indicators; and (3) to present a methodological framework for the identification of LQOs. The implementation of these goals adopted a six-stage procedure based on the use of landscape units: (1) GIS analysis; (2) classification; (3) social survey; (4) expert value judgement; (5) quality assessment; and (6) guidelines formulation. The essence of the research was the presentation of features that determine landscape quality according to public opinion as a set of indicators. The results showed that 80 such indicators were identified, of both a qualitative (49) and a quantitative character (31). Among the analysed units, 60% (18 objects) featured socially expected (and confirmed by experts) levels of landscape quality, and 20% (6 objects) required overall quality improvement in terms of both public and expert opinion. The adopted procedure provides a new tool for integrating social responsibility into environmental management. The advantage of the presented method is the possibility of its application in the territories of various European countries. It is flexible enough to be based on cartographic studies, landscape research methods, and environmental quality standards existing in a given country. Copyright © 2016 Elsevier Ltd. All rights reserved.

  20. Crop Identification Technology Assessment for Remote Sensing (CITARS)

    NASA Technical Reports Server (NTRS)

    Bauer, M. E.; Cary, T. K.; Davis, B. J.; Swain, P. H.

    1975-01-01

    The results of classifications and experiments performed for the Crop Identification Technology Assessment for Remote Sensing (CITARS) project are summarized. Fifteen data sets were classified using two analysis procedures. One procedure used class weights while the other assumed equal probabilities of occurrence for all classes. In addition, 20 data sets were classified using training statistics from another segment or date. The results of both the local and non-local classifications in terms of classification and proportion estimation are presented. Several additional experiments are described which were performed to provide additional understanding of the CITARS results. These experiments investigated alternative analysis procedures, training set selection and size, effects of multitemporal registration, the spectral discriminability of corn, soybeans, and other, and analysis of aircraft multispectral data.

  1. The grain-size lineup: A test of a novel eyewitness identification procedure.

    PubMed

    Horry, Ruth; Brewer, Neil; Weber, Nathan

    2016-04-01

    When making a memorial judgment, respondents can regulate their accuracy by adjusting the precision, or grain size, of their responses. In many circumstances, coarse-grained responses are less informative, but more likely to be accurate, than fine-grained responses. This study describes a novel eyewitness identification procedure, the grain-size lineup, in which participants eliminated any number of individuals from the lineup, creating a choice set of variable size. A decision was considered to be fine-grained if no more than 1 individual was left in the choice set or coarse-grained if more than 1 individual was left in the choice set. Participants (N = 384) watched 2 high-quality or low-quality videotaped mock crimes and then completed 4 standard simultaneous lineups or 4 grain-size lineups (2 target-present and 2 target-absent). There was some evidence of strategic regulation of grain size, as the most difficult lineup was associated with a greater proportion of coarse-grained responses than the other lineups. However, the grain-size lineup did not outperform the standard simultaneous lineup. Fine-grained suspect identifications were no more diagnostic than suspect identifications from standard lineups, whereas coarse-grained suspect identifications carried little probative value. Participants were generally reluctant to provide coarse-grained responses, which may have hampered the utility of the procedure. For a grain-size approach to be useful, participants may need to be trained or instructed to use the coarse-grained option effectively. (c) 2016 APA, all rights reserved).

  2. Integrated identification, modeling and control with applications

    NASA Astrophysics Data System (ADS)

    Shi, Guojun

    This thesis deals with the integration of system design, identification, modeling and control. In particular, six interdisciplinary engineering problems are addressed and investigated. Theoretical results are established and applied to structural vibration reduction and engine control problems. First, the data-based LQG control problem is formulated and solved. It is shown that a state space model is not necessary to solve this problem; rather a finite sequence from the impulse response is the only model data required to synthesize an optimal controller. The new theory avoids unnecessary reliance on a model, required in the conventional design procedure. The infinite horizon model predictive control problem is addressed for multivariable systems. The basic properties of the receding horizon implementation strategy is investigated and the complete framework for solving the problem is established. The new theory allows the accommodation of hard input constraints and time delays. The developed control algorithms guarantee the closed loop stability. A closed loop identification and infinite horizon model predictive control design procedure is established for engine speed regulation. The developed algorithms are tested on the Cummins Engine Simulator and desired results are obtained. A finite signal-to-noise ratio model is considered for noise signals. An information quality index is introduced which measures the essential information precision required for stabilization. The problems of minimum variance control and covariance control are formulated and investigated. Convergent algorithms are developed for solving the problems of interest. The problem of the integrated passive and active control design is addressed in order to improve the overall system performance. A design algorithm is developed, which simultaneously finds: (i) the optimal values of the stiffness and damping ratios for the structure, and (ii) an optimal output variance constrained stabilizing controller such that the active control energy is minimized. A weighted q-Markov COVER method is introduced for identification with measurement noise. The result is use to develop an iterative closed loop identification/control design algorithm. The effectiveness of the algorithm is illustrated by experimental results.

  3. The impact of multiple show-ups on eyewitness decision-making and innocence risk.

    PubMed

    Smith, Andrew M; Bertrand, Michelle; Lindsay, R C L; Kalmet, Natalie; Grossman, Deborah; Provenzano, Daniel

    2014-09-01

    If an eyewitness rejects a show-up, police may respond by finding a new suspect and conducting a second show-up with the same eyewitness. Police may continue finding suspects and conducting show-ups until the eyewitness makes an identification (Study 1). Relatively low criterion-setting eyewitnesses filter themselves out of the multiple show-ups procedure by choosing the first suspect with whom they are presented (Studies 2 and 3). Accordingly, response bias was more stringent on the second show-up when compared with the first, but became no more stringent with additional show-ups. Despite this stringent shift in response bias, innocence risk increased with additional show-ups, as false alarms cumulate (Studies 2 and 3). Although unbiased show-up instructions decreased innocent suspect identifications, the numbers were still discouraging (Study 4). Given the high number of innocent suspects who would be mistakenly identified through the use of multiple show-up procedures, using such identifications as evidence of guilt is questionable. Although evidence of guilt is limited to identifications from a single show-up, practical constraints might sometimes require police to use additional show-ups. Accordingly, we propose a stronger partition between evidentiary and investigative procedures. PsycINFO Database Record (c) 2014 APA, all rights reserved.

  4. Identification of non-technical skills from the resilience engineering perspective: a case study of an electricity distributor.

    PubMed

    Wachs, Priscila; Righi, Angela Weber; Saurin, Tarcisio Abreu

    2012-01-01

    Training in non-technical skills (NTS) does not usually question the design of the work system, and thus focuses narrowly on workers as the unit of analysis. This study discusses how the identification of NTS, a major step for developing an NTS training program, might be re-interpreted from the perspective of resilience engineering (RE). This discussion is based on a case study of identifying NTS for electricians who perform emergency maintenance activities in an electricity distribution power line. The results of the case study point out that four data analysis procedures might operationalize the RE perspective: (a) identifying factors that make the work difficult and which could be integrated into NTS training scenarios; (b) identifying recommendations for re-designing the system, in order either to reduce or eliminate the need for NTS; (c) classifying the NTS into pragmatic categories, which are meaningful for workers; and (d) regarding the identification of NTS as an opportunity to give visibility to adaptations carried out by workers.

  5. An artificial tongue fluorescent sensor array for identification and quantitation of various heavy metal ions.

    PubMed

    Xu, Wang; Ren, Changliang; Teoh, Chai Lean; Peng, Juanjuan; Gadre, Shubhankar Haribhau; Rhee, Hyun-Woo; Lee, Chi-Lik Ken; Chang, Young-Tae

    2014-09-02

    Herein, a small-molecule fluorescent sensor array for rapid identification of seven heavy metal ions was designed and synthesized, with its sensing mechanism mimicking that of a tongue. The photoinduced electron transfer and intramolecular charge transfer mechanism result in combinatorial interactions between sensor array and heavy metal ions, which lead to diversified fluorescence wavelength shifts and emission intensity changes. Upon principle component analysis (PCA), this result renders clear identification of each heavy metal ion on a 3D spatial dispersion graph. Further exploration provides a concentration-dependent pattern, allowing both qualitative and quantitative measurements of heavy metal ions. On the basis of this information, a "safe-zone" concept was proposed, which provides rapid exclusion of versatile hazardous species from clean water samples based on toxicity characteristic leaching procedure standards. This type of small-molecule fluorescent sensor array could open a new avenue for multiple heavy metal ion detection and simplified water quality analysis.

  6. Automated extraction of family history information from clinical notes.

    PubMed

    Bill, Robert; Pakhomov, Serguei; Chen, Elizabeth S; Winden, Tamara J; Carter, Elizabeth W; Melton, Genevieve B

    2014-01-01

    Despite increased functionality for obtaining family history in a structured format within electronic health record systems, clinical notes often still contain this information. We developed and evaluated an Unstructured Information Management Application (UIMA)-based natural language processing (NLP) module for automated extraction of family history information with functionality for identifying statements, observations (e.g., disease or procedure), relative or side of family with attributes (i.e., vital status, age of diagnosis, certainty, and negation), and predication ("indicator phrases"), the latter of which was used to establish relationships between observations and family member. The family history NLP system demonstrated F-scores of 66.9, 92.4, 82.9, 57.3, 97.7, and 61.9 for detection of family history statements, family member identification, observation identification, negation identification, vital status, and overall extraction of the predications between family members and observations, respectively. While the system performed well for detection of family history statements and predication constituents, further work is needed to improve extraction of certainty and temporal modifications.

  7. Automated Extraction of Family History Information from Clinical Notes

    PubMed Central

    Bill, Robert; Pakhomov, Serguei; Chen, Elizabeth S.; Winden, Tamara J.; Carter, Elizabeth W.; Melton, Genevieve B.

    2014-01-01

    Despite increased functionality for obtaining family history in a structured format within electronic health record systems, clinical notes often still contain this information. We developed and evaluated an Unstructured Information Management Application (UIMA)-based natural language processing (NLP) module for automated extraction of family history information with functionality for identifying statements, observations (e.g., disease or procedure), relative or side of family with attributes (i.e., vital status, age of diagnosis, certainty, and negation), and predication (“indicator phrases”), the latter of which was used to establish relationships between observations and family member. The family history NLP system demonstrated F-scores of 66.9, 92.4, 82.9, 57.3, 97.7, and 61.9 for detection of family history statements, family member identification, observation identification, negation identification, vital status, and overall extraction of the predications between family members and observations, respectively. While the system performed well for detection of family history statements and predication constituents, further work is needed to improve extraction of certainty and temporal modifications. PMID:25954443

  8. Modern control concepts in hydrology

    NASA Technical Reports Server (NTRS)

    Duong, N.; Johnson, G. R.; Winn, C. B.

    1974-01-01

    Two approaches to an identification problem in hydrology are presented based upon concepts from modern control and estimation theory. The first approach treats the identification of unknown parameters in a hydrologic system subject to noisy inputs as an adaptive linear stochastic control problem; the second approach alters the model equation to account for the random part in the inputs, and then uses a nonlinear estimation scheme to estimate the unknown parameters. Both approaches use state-space concepts. The identification schemes are sequential and adaptive and can handle either time invariant or time dependent parameters. They are used to identify parameters in the Prasad model of rainfall-runoff. The results obtained are encouraging and conform with results from two previous studies; the first using numerical integration of the model equation along with a trial-and-error procedure, and the second, by using a quasi-linearization technique. The proposed approaches offer a systematic way of analyzing the rainfall-runoff process when the input data are imbedded in noise.

  9. Filtrates and Residues: Chlorophyll Separation and Spectral Identification.

    ERIC Educational Resources Information Center

    Diehl-Jones, Susan M.

    1984-01-01

    Describes an experiment in which students become familiar with the organic chemistry extraction process, perform analyses by thin layer and column chromatography, and use the absorption spectrum (obtained from a spectrophotometer) for identification. List of materials needed and procedures used are provided. (JN)

  10. Identification of Some Zeolite Group Minerals by Application of Artificial Neural Network and Decision Tree Algorithm Based on SEM-EDS Data

    NASA Astrophysics Data System (ADS)

    Akkaş, Efe; Evren Çubukçu, H.; Akin, Lutfiye; Erkut, Volkan; Yurdakul, Yasin; Karayigit, Ali Ihsan

    2016-04-01

    Identification of zeolite group minerals is complicated due to their similar chemical formulas and habits. Although the morphologies of various zeolite crystals can be recognized under Scanning Electron Microscope (SEM), it is relatively more challenging and problematic process to identify zeolites using their mineral chemical data. SEMs integrated with energy dispersive X-ray spectrometers (EDS) provide fast and reliable chemical data of minerals. However, considering elemental similarities of characteristic chemical formulae of zeolite species (e.g. Clinoptilolite ((Na,K,Ca)2 -3Al3(Al,Si)2Si13O3612H2O) and Erionite ((Na2,K2,Ca)2Al4Si14O36ṡ15H2O)) EDS data alone does not seem to be sufficient for correct identification. Furthermore, the physical properties of the specimen (e.g. roughness, electrical conductivity) and the applied analytical conditions (e.g. accelerating voltage, beam current, spot size) of the SEM-EDS should be uniform in order to obtain reliable elemental results of minerals having high alkali (Na, K) and H2O (approx. %14-18) contents. This study which was funded by The Scientific and Technological Research Council of Turkey (TUBITAK Project No: 113Y439), aims to construct a database as large as possible for various zeolite minerals and to develop a general prediction model for the identification of zeolite minerals using SEM-EDS data. For this purpose, an artificial neural network and rule based decision tree algorithm were employed. Throughout the analyses, a total of 1850 chemical data were collected from four distinct zeolite species, (Clinoptilolite-Heulandite, Erionite, Analcime and Mordenite) observed in various rocks (e.g. coals, pyroclastics). In order to obtain a representative training data set for each minerals, a selection procedure for reference mineral analyses was applied. During the selection procedure, SEM based crystal morphology data, XRD spectra and re-calculated cationic distribution, obtained by EDS have been used for the characterization of the training set. Consequently, for each zeolite species 250 EDS data (as elemental intensities) used for training and 200 ±50 analyses were tested. Finally, two prediction models were developed. The constructed models with various cross-correlation values (r) yielded an average accuracy of >91% for the best predictions using C5.0 Decision Tree algorithm and back propagation artificial neural network. Despite having similar accuracies, the developed models exhibit different prediction behaviors for some zeolite minerals. The results demonstrate that artificial neural network as a nonlinear tool and decision tree algorithm as a rule based prediction model would be employed to provide considerably efficient and reliable identification/classification of some zeolite minerals regardless of their similar elemental compositions. Keywords: mineral identification; zeolites; energy dispersive spectrometry; artificial neural networks; decision tree.

  11. Endoscopic Sex Identification in Chelonians and Birds (Psittacines, Passerines, and Raptors).

    PubMed

    Divers, Stephen J

    2015-09-01

    Despite the advent of DNA probes for sex identification of many avian and some reptile species, clinicians involved with zoos, conservation projects, or breeders may still be asked to perform "surgical sexing." This article describes the practical approach to performing endoscopic sex identification in psittacines and chelonians, including patient preparation, anesthesia, and endoscopic procedure. Copyright © 2015 Elsevier Inc. All rights reserved.

  12. THE 1955 UNIVERSITY OF UTAH RESEARCH CONFERENCE ON THE IDENTIFICATION OF CREATIVE SCIENTIFIC TALENT (1ST, BRIGHTON, AUGUST 27-30, 1955.)

    ERIC Educational Resources Information Center

    TAYLOR, CALVIN W.

    PAPERS AND COMMITTEE REPORTS ARE INCLUDED IN THIS REPORT OF A 1955 CONFERENCE ON THE IDENTIFICATION OF CREATIVE SCIENCE TALENT. MOST PAPERS DEAL WITH THE THEORETICAL INTERPRETATION OF CREATIVITY AND ITS MEASUREMENT. SOME ARE REVIEWS OF RESEARCH RELATED TO THE IDENTIFICATION OF CREATIVE INDIVIDUALS AND PROCEDURES USED BY VARIOUS ORGANIZATIONS IN…

  13. Evaluation of intranuclear BrdU detection procedures for use in multicolor flow cytometry*

    PubMed Central

    Rothaeusler, Kristina; Baumgarth, Nicole

    2010-01-01

    Background Measurement of cell proliferation via BrdU incorporation in combination with multicolor cell surface staining would facilitate studies on cell subsets that require multiple markers for their identification. However, the extent to which the often harsh cell preparation procedures required affect the staining quality of more recently developed fluorescent dyes has not been assessed. Methods Three cell preparation protocols for BrdU measurement were compared for their ability to maintain fluorescent surface staining and scatter parameters of in vivo BrdU-labeled cells by flow cytometry. A 10-color fluorescent panel was developed to test the quality of surface staining following cell treatment and the ability to perform BrdU measurements on even small B lymphocyte subsets. Results All cell preparation procedures affected the quality of fluorescent and/or scatter parameters to varying degrees. Paraformaldehyde / saponin-based procedures preserved sufficient fluorescent surface staining to determine BrdU incorporation rates among all splenic B cell subsets, including B-1a cells, which constitute roughly 0.5% of cells. Turnover rates of B-1a cells were similar to immature B cells and higher than those of the other mature B cell subsets. Conclusion Paraformaldehyde / saponin-based cell preparation procedures facilitate detailed cell turnover studies on small cell subsets in vivo, revealing new functional information on rare cell populations. PMID:16538653

  14. Reducing time to identification of aerobic bacteria and fastidious micro-organisms in positive blood cultures.

    PubMed

    Intra, J; Sala, M R; Falbo, R; Cappellini, F; Brambilla, P

    2016-12-01

    Rapid and early identification of micro-organisms in blood has a key role in the diagnosis of a febrile patient, in particular, in guiding the clinician to define the correct antibiotic therapy. This study presents a simple and very fast method with high performances for identifying bacteria by matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) after only 4 h of incubation. We used early bacterial growth on PolyViteX chocolate agar plates inoculated with five drops of blood-broth medium deposited in the same point and spread with a sterile loop, followed by a direct transfer procedure on MALDI-TOF MS target slides without additional modification. Ninety-nine percentage of aerobic bacteria were correctly identified from 600 monomicrobial-positive blood cultures. This procedure allowed obtaining the correct identification of fastidious pathogens, such as Streptococcus pneumoniae, Neisseria meningitidis and Haemophilus influenzae that need complex nutritional and environmental requirements in order to grow. Compared to the traditional pathogen identification from blood cultures that takes over 24 h, the reliability of results, rapid performance and suitability of this protocol allowed a more rapid administration of optimal antimicrobial treatment in the patients. Bloodstream infections are serious conditions with a high mortality and morbidity rate. Rapid identification of pathogens and appropriate antimicrobial therapy have a key role for successful patient outcome. In this work, we developed a rapid, simplified, accurate, and efficient method, reaching 99 % identification of aerobic bacteria from monomicrobial-positive blood cultures by using early growth on enriched medium, direct transfer to target plate without additional procedures, matrix-assisted laser desorption ionization-time of flight mass spectrometry and SARAMIS database. The application of this protocol allows to anticipate appropriate antibiotic therapy. © 2016 The Society for Applied Microbiology.

  15. Exploring MALDI-TOF MS approach for a rapid identification of Mycobacterium avium ssp. paratuberculosis field isolates.

    PubMed

    Ricchi, M; Mazzarelli, A; Piscini, A; Di Caro, A; Cannas, A; Leo, S; Russo, S; Arrigoni, N

    2017-03-01

    The aim of the study was to explore the suitability of matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry (MALDI-TOF MS) for a rapid and correct identification of Mycobacterium avium ssp. paratuberculosis (MAP) field isolates. MALDI-TOF MS approach is becoming one of the most popular tests for the identification of intact bacterial cells which has been shown to be fast and reliable. For this purpose, 36 MAP field isolates were analysed through MALDI-TOF MS and the spectra compared with two different databases: one provided by the vendor of the system employed (Biotyper ver. 3·0; Bruker Daltonics) and a homemade database containing spectra from both tuberculous and nontuberculous Mycobacteria. Moreover, principal component analysis procedure was employed to confirm the ability of MALDI-TOF MS to discriminate between very closely related subspecies. Our results suggest MAP can be differentiated from other Mycobacterium species, both when the species are very close (M. intracellulare) and when belonging to different subspecies (M. avium ssp. avium and M. avium ssp. silvaticum). The procedure applied is fast, easy to perform, and achieves an earlier accurate species identification of MAP and nontuberculous Mycobacteria in comparison to other procedures. The gold standard test for the diagnosis of paratuberculosis is still isolation of MAP by cultural methods, but additional assays, such as qPCR and subculturing for determination of mycobactin dependency are required to confirm its identification. We have provided here evidence pertaining to the usefulness of MALDI-TOF MS approach for a rapid identification of this mycobacterium among other members of M. avium complex. © 2016 The Society for Applied Microbiology.

  16. Fine-tuned evaluation of olfactory function in patients operated for nasal polyposis.

    PubMed

    Sonnet, Marie-Hortense; Nguyen, Duc Trung; Nguyen-Thi, Phi-Linh; Arous, Fabien; Jankowski, Roger; Rumeau, Cécile

    2017-07-01

    Given the forced-choice procedure of the identification test, patients with profound anosmia are more likely to have higher identification scores by chance than patients with hyposmia or normosmia. This may be a confusing factor when assessing the sense of smell, which alters the appreciation of real olfaction improvement. The aim of this study was to fine-tune the results of the identification Sniffin' Sticks test before and 6 weeks after surgery using the real identification score. A total of 133 patients underwent the Identification (I) and Threshold (T) tests the day before and 6 weeks after nasalization surgery. The scores of the identification test, called I G (global identification), were ranked from 0 to 16. Patients had to specify if their forced-choice answers were given either surely or randomly, called I H (hazard identification). The real score of identification I R was obtained as follow: I R  = I G  - I H . Patients with an immeasurable threshold according to the T test were more prone to give randomly correct answers. On the basis of I G scores, 43.6% of patients remained hypo-anosmic after surgery compared to 72.9% before surgery. Using I R scores, only 3.8% of patients remained anosmic (I R  = 0) at 6 weeks after surgery. Hence, patients with real anosmia (I R  = 0) were less prone to improve their olfaction than patients with I R  > 0. The analysis of random factor when using identification test allows differentiating a real anosmia from a hyposmia. An I G  ≤ 4 could be considered as a profound/real anosmia or a severe hyposmia. This procedure cannot, however, replace the forced-choice method in odor identification testing.

  17. Alternatives for Assessing the Presence of Advanced Intellectual Abilities in Young Children.

    ERIC Educational Resources Information Center

    White, C. Stephen

    1985-01-01

    Assessment procedures aimed at differentiating intellectually gifted young children from their nongifted peers are proposed as alternatives to traditional identification procedures. Suggested is measuring the use of strategic behavior to solve novel problems. (Author/CL)

  18. 18 CFR 50.5 - Pre-filing procedures.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... ELECTRIC TRANSMISSION FACILITIES § 50.5 Pre-filing procedures. (a) Introduction. Any applicant seeking a... consultations, project engineering, route planning, environmental and engineering contractor engagement... proceeding. This description also must include the identification of the environmental and engineering firms...

  19. Personal identification based on blood vessels of retinal fundus images

    NASA Astrophysics Data System (ADS)

    Fukuta, Keisuke; Nakagawa, Toshiaki; Hayashi, Yoshinori; Hatanaka, Yuji; Hara, Takeshi; Fujita, Hiroshi

    2008-03-01

    Biometric technique has been implemented instead of conventional identification methods such as password in computer, automatic teller machine (ATM), and entrance and exit management system. We propose a personal identification (PI) system using color retinal fundus images which are unique to each individual. The proposed procedure for identification is based on comparison of an input fundus image with reference fundus images in the database. In the first step, registration between the input image and the reference image is performed. The step includes translational and rotational movement. The PI is based on the measure of similarity between blood vessel images generated from the input and reference images. The similarity measure is defined as the cross-correlation coefficient calculated from the pixel values. When the similarity is greater than a predetermined threshold, the input image is identified. This means both the input and the reference images are associated to the same person. Four hundred sixty-two fundus images including forty-one same-person's image pairs were used for the estimation of the proposed technique. The false rejection rate and the false acceptance rate were 9.9×10 -5% and 4.3×10 -5%, respectively. The results indicate that the proposed method has a higher performance than other biometrics except for DNA. To be used for practical application in the public, the device which can take retinal fundus images easily is needed. The proposed method is applied to not only the PI but also the system which warns about misfiling of fundus images in medical facilities.

  20. [Special application of matrix-assisted laser desorption ionization time-of-flight mass spectrometry in clinical microbiological diagnostics].

    PubMed

    Nagy, Erzsébet; Abrók, Marianna; Bartha, Noémi; Bereczki, László; Juhász, Emese; Kardos, Gábor; Kristóf, Katalin; Miszti, Cecilia; Urbán, Edit

    2014-09-21

    Matrix-assisted laser desorption ionization time-of-flight mass spectrometry as a new possibility for rapid identification of bacteria and fungi revolutionized the clinical microbiological diagnostics. It has an extreme importance in the routine microbiological laboratories, as identification of the pathogenic species rapidly will influence antibiotic selection before the final determination of antibiotic resistance of the isolate. The classical methods for identification of bacteria or fungi, based on biochemical tests, are influenced by many environmental factors. The matrix-assisted laser desorption ionization time-of-flight mass spectrometry is a rapid method which is able to identify a great variety of the isolated bacteria and fungi based on the composition of conserved ribosomal proteins. Recently several other applications of the method have also been investigated such as direct identification of pathogens from the positive blood cultures. There are possibilities to identify bacteria from the urine samples in urinary tract infection or from other sterile body fluids. Using selective enrichment broth Salmonella sp from the stool samples can be identified more rapidly, too. The extended spectrum beta-lactamase or carbapenemase production of the isolated bacteria can be also detected by this method helping the antibiotic selection in some cases. Matrix-assisted laser desorption ionization time-of-flight mass spectrometry based methods are suitable to investigate changes in deoxyribonucleic acid or ribonucleic acid, to carry out rapid antibiotic resistance determination or other proteomic analysis. The aim of this paper is to give an overview about present possibilities of using this technique in the clinical microbiological routine procedures.

  1. U.S.-MEXICO BORDER PROGRAM ARIZONA BORDER STUDY--STANDARD OPERATING PROCEDURE FOR COLLECTION, STORAGE, AND SHIPMENT OF UNKNOWN SUBSTANCES (UA-F-25.0)

    EPA Science Inventory

    The purpose of this SOP is to describe how to collect, store, and ship unknown substances for chemical analysis and identification. This SOP provides a brief description of the sample containers, collection, storage, shipping and custody procedures. This procedure was followed ...

  2. Pain management in the neonatal piglet during routine management procedures. Part 2: Grading the quality of evidence and the strength of recommendations

    USDA-ARS?s Scientific Manuscript database

    Piglets reared in swine production in the US undergo painful procedures that include castration, tail docking, teeth clipping, and identification with ear notching or tagging. These procedures are usually performed without pain mitigation. The objective of this project was to develop recommendations...

  3. Considerations for Incorporating Bioavailability in Effect-Directed Analysis and Toxicity Identification Evaluation.

    EPA Science Inventory

    In order to avoid a bias toward highly toxic but poorly bioavailable compounds in the effect-directed analysis (EDA) of soils and sediments, approaches are discussed to consider bioavailability in EDA procedures. In parallel, complimentary approaches for making toxicity identific...

  4. 5 CFR 1205.11 - Access to Board records.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 1205.11 Administrative Personnel MERIT SYSTEMS PROTECTION BOARD ORGANIZATION AND PROCEDURES PRIVACY ACT... authenticated copy of: (i) The birth certificate of the minor child, and (ii) The court document appointing the... is a “PRIVACY ACT REQUEST”. (c) Identification. Each submission must follow the identification...

  5. 5 CFR 1205.11 - Access to Board records.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 1205.11 Administrative Personnel MERIT SYSTEMS PROTECTION BOARD ORGANIZATION AND PROCEDURES PRIVACY ACT... authenticated copy of: (i) The birth certificate of the minor child, and (ii) The court document appointing the... is a “PRIVACY ACT REQUEST”. (c) Identification. Each submission must follow the identification...

  6. Assessment of Supercritical Fluid Extraction Use in Whole Sediment Toxicity Identification Evaluations

    EPA Science Inventory

    In this investigation, supercritical fluid extraction (SFE) with pure CO2 was assessed as a confirmatory tool in Phase III of whole sediment toxicity identification evaluations (TIEs). The SFE procedure was assessed on two reference sediments and three contaminated sediments usi...

  7. Producing patient-avatar identification in animation video information on spinal anesthesia by different narrative strategies.

    PubMed

    Høybye, Mette Terp; Vesterby, Martin; Jørgensen, Lene Bastrup

    2016-06-01

    Visual approaches to health information reduce complexity and may bridge challenges in health literacy. But the mechanisms and meanings of using animated video in communication with patients undergoing surgery are not well described. By comparing two versions of a two-dimensional animated video on spinal anesthesia, this study tested the patient-avatar identification within two different narrative models. To explore the perspectives of total hip arthroplasty, we employed qualitative methods of interviews and ethnographic observation. The animated presentation of the spinal anesthesia procedure was immediately recognized by all participants as reflecting their experience of the procedure independent of the narrative form. The avatar gender did not affect this identification. We found no preference for either narrative form. This study supports the potential of animation video in health informatics as a didactic model for qualifying patient behavior. Animation video creates a high degree of identification that may work to reduce pre-surgical anxiety. © The Author(s) 2014.

  8. Determination of vehicle density from traffic images at day and nighttime

    NASA Astrophysics Data System (ADS)

    Mehrübeoğlu, Mehrübe; McLauchlan, Lifford

    2007-02-01

    In this paper we extend our previous work to address vehicle differentiation in traffic density computations1. The main goal of this work is to create vehicle density history for given roads under different weather or light conditions and at different times of the day. Vehicle differentiation is important to account for connected or otherwise long vehicles, such as trucks or tankers, which lead to over-counting with the original algorithm. Average vehicle size in pixels, given the magnification within the field of view for a particular camera, is used to separate regular cars and long vehicles. A separate algorithm and procedure have been developed to determine traffic density after dark when the vehicle headlights are turned on. Nighttime vehicle recognition utilizes blob analysis based on head/taillight images. The high intensity of vehicle lights are identified in binary images for nighttime vehicle detection. The stationary traffic image frames are downloaded from the internet as they are updated. The procedures are implemented in MATLAB. The results of both nighttime traffic density and daytime long vehicle identification algorithms are described in this paper. The determination of nighttime traffic density, and identification of long vehicles at daytime are improvements over the original work1.

  9. Identification of water soluble and particle bound compounds causing sublethal toxic effects. A field study on sediments affected by a chlor-alkali industry.

    PubMed

    Bosch, Carme; Olivares, Alba; Faria, Melissa; Navas, Jose M; del Olmo, Iván; Grimalt, Joan O; Piña, Benjamín; Barata, Carlos

    2009-08-13

    A combination of cost effective sublethal Daphnia magna feeding tests, yeast- and cell culture-based bioassays and Toxicity Identification Evaluation (TIE) procedures was used to characterize toxic compounds within sediments collected in a river area under the influence of the effluents from a chlor-alkali industry (Ebro River, NE Spain). Tests were designed to measure and identify toxic compounds in the particulate and filtered water fractions of sediment elutriates. The combined use of bioassays responding to elutriates and dioxin-like compounds evidenced the existence of three major groups of hazardous contaminants in the most contaminated site: (A) metals such as cadmium and mercury bound to sediment fine particles that could be easily resuspended and moved downstream, (B) soluble compounds (presumably, lye) able to alkalinize water to toxic levels, and (C) organochlorine compounds with high dioxin-like activity. These results provided evidence that elutriate D. magna feeding responses can be used as surrogate assays for more tedious chronic whole sediment tests, and that the incorporation of such tests in sediment TIE procedures may improve the ability to identify the toxicity of particle-bound and water-soluble contaminants in sediments.

  10. Ultra-high performance supercritical fluid chromatography-mass spectrometry procedure for analysis of monosaccharides from plant gum binders.

    PubMed

    Pauk, Volodymyr; Pluháček, Tomáš; Havlíček, Vladimír; Lemr, Karel

    2017-10-09

    The ultra-high performance supercritical fluid chromatography-mass spectrometry (UHPSFC/MS) procedure for analysis of native monosaccharides was developed. Chromatographic conditions were investigated to separate a mixture of four hexoses, three pentoses, two deoxyhexoses and two uronic acids. Increasing water content in methanol modifier to 5% and formic acid to 4% improved peak shapes of neutral monosaccharides and allowed complete elution of highly polar uronic acids in a single run. An Acquity HSS C18SB column outperformed other three tested stationary phases (BEH (silica), BEH 2-ethylpyridine, CSH Fluoro-Phenyl) in terms of separation of isomers and analysis time (4.5 min). Limits of detection were in the range 0.01-0.12 ng μL -1 . Owing to separation of anomers, identification of critical pairs (arabinose-xylose and glucose-galactose) was possible. Feasibility of the new method was demonstrated on plant-derived polysaccharide binders. Samples of watercolor paints, painted paper and three plant gums widely encountered in painting media (Arabic, cherry and tragacanth) were decomposed prior the analysis by microwave-assisted hydrolysis at 40 bar initial pressure using 2 mol L -1 trifluoroacetic acid. Among tested temperatures, 120 °C ensured appropriate hydrolysis efficiency for different types of gum and avoided excessive degradation of labile monosaccharides. Procedure recovery tested on gum Arabic was 101% with an RSD below 8%. Aqueous hydrolysates containing monosaccharides in different ratios specific to each type of plant gum were diluted or analyzed directly. Filtration of samples before hydrolysis reduced interferences from a paper support and identification of gum Arabic in watercolor-painted paper samples was demonstrated. Successful identification of pure gum Arabic was confirmed for sample quantities as little as 1 μg. Two classification approaches were compared and principal component analysis was superior to analysis based on peak area ratios of monosaccharides. The proposed procedure using UHPSFC/MS represents an interesting alternative which can compete with other chromatographic methods in the field of saccharide analysis in terms of speed, sensitivity and simplicity of workflow. Copyright © 2017 Elsevier B.V. All rights reserved.

  11. Φ-score: A cell-to-cell phenotypic scoring method for sensitive and selective hit discovery in cell-based assays.

    PubMed

    Guyon, Laurent; Lajaunie, Christian; Fer, Frédéric; Bhajun, Ricky; Sulpice, Eric; Pinna, Guillaume; Campalans, Anna; Radicella, J Pablo; Rouillier, Philippe; Mary, Mélissa; Combe, Stéphanie; Obeid, Patricia; Vert, Jean-Philippe; Gidrol, Xavier

    2015-09-18

    Phenotypic screening monitors phenotypic changes induced by perturbations, including those generated by drugs or RNA interference. Currently-used methods for scoring screen hits have proven to be problematic, particularly when applied to physiologically relevant conditions such as low cell numbers or inefficient transfection. Here, we describe the Φ-score, which is a novel scoring method for the identification of phenotypic modifiers or hits in cell-based screens. Φ-score performance was assessed with simulations, a validation experiment and its application to gene identification in a large-scale RNAi screen. Using robust statistics and a variance model, we demonstrated that the Φ-score showed better sensitivity, selectivity and reproducibility compared to classical approaches. The improved performance of the Φ-score paves the way for cell-based screening of primary cells, which are often difficult to obtain from patients in sufficient numbers. We also describe a dedicated merging procedure to pool scores from small interfering RNAs targeting the same gene so as to provide improved visualization and hit selection.

  12. Conceptualising the effectiveness of impact assessment processes

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Chanchitpricha, Chaunjit, E-mail: chaunjit@g.sut.ac.th; Bond, Alan, E-mail: alan.bond@uea.ac.uk; Unit for Environmental Sciences and Management School of Geo and Spatial Sciences, Internal Box 375, North West University

    2013-11-15

    This paper aims at conceptualising the effectiveness of impact assessment processes through the development of a literature-based framework of criteria to measure impact assessment effectiveness. Four categories of effectiveness were established: procedural, substantive, transactive and normative, each containing a number of criteria; no studies have previously brought together all four of these categories into such a comprehensive, criteria-based framework and undertaken systematic evaluation of practice. The criteria can be mapped within a cycle/or cycles of evaluation, based on the ‘logic model’, at the stages of input, process, output and outcome to enable the identification of connections between the criteria acrossmore » the categories of effectiveness. This framework is considered to have potential application in measuring the effectiveness of many impact assessment processes, including strategic environmental assessment (SEA), environmental impact assessment (EIA), social impact assessment (SIA) and health impact assessment (HIA). -- Highlights: • Conceptualising effectiveness of impact assessment processes. • Identification of factors influencing effectiveness of impact assessment processes. • Development of criteria within a framework for evaluating IA effectiveness. • Applying the logic model to examine connections between effectiveness criteria.« less

  13. Φ-score: A cell-to-cell phenotypic scoring method for sensitive and selective hit discovery in cell-based assays

    PubMed Central

    Guyon, Laurent; Lajaunie, Christian; fer, Frédéric; bhajun, Ricky; sulpice, Eric; pinna, Guillaume; campalans, Anna; radicella, J. Pablo; rouillier, Philippe; mary, Mélissa; combe, Stéphanie; obeid, Patricia; vert, Jean-Philippe; gidrol, Xavier

    2015-01-01

    Phenotypic screening monitors phenotypic changes induced by perturbations, including those generated by drugs or RNA interference. Currently-used methods for scoring screen hits have proven to be problematic, particularly when applied to physiologically relevant conditions such as low cell numbers or inefficient transfection. Here, we describe the Φ-score, which is a novel scoring method for the identification of phenotypic modifiers or hits in cell-based screens. Φ-score performance was assessed with simulations, a validation experiment and its application to gene identification in a large-scale RNAi screen. Using robust statistics and a variance model, we demonstrated that the Φ-score showed better sensitivity, selectivity and reproducibility compared to classical approaches. The improved performance of the Φ-score paves the way for cell-based screening of primary cells, which are often difficult to obtain from patients in sufficient numbers. We also describe a dedicated merging procedure to pool scores from small interfering RNAs targeting the same gene so as to provide improved visualization and hit selection. PMID:26382112

  14. Proteomic analysis of exosomes from human neural stem cells by flow field-flow fractionation and nanoflow liquid chromatography-tandem mass spectrometry.

    PubMed

    Kang, Dukjin; Oh, Sunok; Ahn, Sung-Min; Lee, Bong-Hee; Moon, Myeong Hee

    2008-08-01

    Exosomes, small membrane vesicles secreted by a multitude of cell types, are involved in a wide range of physiological roles such as intercellular communication, membrane exchange between cells, and degradation as an alternative to lysosomes. Because of the small size of exosomes (30-100 nm) and the limitations of common separation procedures including ultracentrifugation and flow cytometry, size-based fractionation of exosomes has been challenging. In this study, we used flow field-flow fractionation (FlFFF) to fractionate exosomes according to differences in hydrodynamic diameter. The exosome fractions collected from FlFFF runs were examined by transmission electron microscopy (TEM) to morphologically confirm their identification as exosomes. Exosomal lysates of each fraction were digested and analyzed using nanoflow LC-ESI-MS-MS for protein identification. FIFFF, coupled with mass spectrometry, allows nanoscale size-based fractionation of exosomes and is more applicable to primary cells and stem cells since it requires much less starting material than conventional gel-based separation, in-gel digestion and the MS-MS method.

  15. Molecular identification and typing of Burkholderia pseudomallei and Burkholderia mallei: when is enough enough?

    PubMed

    Antonov, Valery A; Tkachenko, Galina A; Altukhova, Viktoriya V; Savchenko, Sergey S; Zinchenko, Olga V; Viktorov, Dmitry V; Zamaraev, Valery S; Ilyukhin, Vladimir I; Alekseev, Vladimir V

    2008-12-01

    Burkholderia mallei and B. pseudomallei are highly pathogenic microorganisms for both humans and animals. Moreover, they are regarded as potential agents of bioterrorism. Thus, rapid and unequivocal detection and identification of these dangerous pathogens is critical. In the present study, we describe the use of an optimized protocol for the early diagnosis of experimental glanders and melioidosis and for the rapid differentiation and typing of Burkholderia strains. This experience with PCR-based identification methods indicates that single PCR targets (23S and 16S rRNA genes, 16S-23S intergenic region, fliC and type III secretion gene cluster) should be used with caution for identification of B. mallei and B. pseudomallei, and need to be used alongside molecular methods such as gene sequencing. Several molecular typing procedures have been used to identify genetically related B. pseudomallei and B. mallei isolates, including ribotyping, pulsed-field gel electrophoresis and multilocus sequence typing. However, these methods are time consuming and technically challenging for many laboratories. RAPD, variable amplicon typing scheme, Rep-PCR, BOX-PCR and multiple-locus variable-number tandem repeat analysis have been recommended by us for the rapid differentiation of B. mallei and B. pseudomallei strains.

  16. Patient Evaluation and Preparation in Vascular and Interventional Radiology: What Every Interventional Radiologist Should Know (Part 2: Patient Preparation and Medications)

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Taslakian, Bedros, E-mail: btaslakian@gmail.com; Sebaaly, Mikhael Georges, E-mail: ms246@aub.edu.lb; Al-Kutoubi, Aghiad, E-mail: mk00@aub.edu.lb

    2016-04-15

    Performing an interventional procedure imposes a commitment on interventional radiologists to conduct the initial patient assessment, determine the best course of therapy, and provide long-term care. Patient care before and after an interventional procedure, identification, and management of early and delayed complications of various procedures are equal in importance to the procedure itself. In this second part, we complete the comprehensive, methodical review of pre-procedural care and patient preparation before vascular and interventional radiology procedures.

  17. Patient Evaluation and Preparation in Vascular and Interventional Radiology: What Every Interventional Radiologist Should Know (Part 2: Patient Preparation and Medications).

    PubMed

    Taslakian, Bedros; Sebaaly, Mikhael Georges; Al-Kutoubi, Aghiad

    2016-04-01

    Performing an interventional procedure imposes a commitment on interventional radiologists to conduct the initial patient assessment, determine the best course of therapy, and provide long-term care. Patient care before and after an interventional procedure, identification, and management of early and delayed complications of various procedures are equal in importance to the procedure itself. In this second part, we complete the comprehensive, methodical review of pre-procedural care and patient preparation before vascular and interventional radiology procedures.

  18. Multi-Agent Diagnosis and Control of an Air Revitalization System for Life Support in Space

    NASA Technical Reports Server (NTRS)

    Malin, Jane T.; Kowing, Jeffrey; Nieten, Joseph; Graham, Jeffrey s.; Schreckenghost, Debra; Bonasso, Pete; Fleming, Land D.; MacMahon, Matt; Thronesbery, Carroll

    2000-01-01

    An architecture of interoperating agents has been developed to provide control and fault management for advanced life support systems in space. In this adjustable autonomy architecture, software agents coordinate with human agents and provide support in novel fault management situations. This architecture combines the Livingstone model-based mode identification and reconfiguration (MIR) system with the 3T architecture for autonomous flexible command and control. The MIR software agent performs model-based state identification and diagnosis. MIR identifies novel recovery configurations and the set of commands required for the recovery. The AZT procedural executive and the human operator use the diagnoses and recovery recommendations, and provide command sequencing. User interface extensions have been developed to support human monitoring of both AZT and MIR data and activities. This architecture has been demonstrated performing control and fault management for an oxygen production system for air revitalization in space. The software operates in a dynamic simulation testbed.

  19. Automatic load forecasting. Final report

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Nelson, D.J.; Vemuri, S.

    A method which lends itself to on-line forecasting of hourly electric loads is presented and the results of its use are compared to models developed using the Box-Jenkins method. The method consists of processing the historical hourly loads with a sequential least-squares estimator to identify a finite order autoregressive model which in turn is used to obtain a parsimonious autoregressive-moving average model. A procedure is also defined for incorporating temperature as a variable to improve forecasts where loads are temperature dependent. The method presented has several advantages in comparison to the Box-Jenkins method including much less human intervention and improvedmore » model identification. The method has been tested using three-hourly data from the Lincoln Electric System, Lincoln, Nebraska. In the exhaustive analyses performed on this data base this method produced significantly better results than the Box-Jenkins method. The method also proved to be more robust in that greater confidence could be placed in the accuracy of models based upon the various measures available at the identification stage.« less

  20. Identification of lymphogranuloma venereum-associated Chlamydia trachomatis serovars by fluorescence in situ hybridisation--a proof-of-principle analysis.

    PubMed

    Frickmann, Hagen; Essig, Andreas; Poppert, Sven

    2014-04-01

    We describe a proof-of-principle evaluation of a fluorescence in situ hybridisation (FISH) procedure to identify Chlamydia trachomatis serovars L1-L3, the causative agents of lymphogranuloma venereum, in cell cultures based on newly designed DNA probes. Rapid and easy-to-perform FISH could facilitate the diagnosis of lymphogranuloma venereum without nucleic acid amplification or serotyping, but requires broader evaluation studies, for example, in tropical high-endemicity regions. © 2014 John Wiley & Sons Ltd.

  1. Functional Metagenomics: Construction and High-Throughput Screening of Fosmid Libraries for Discovery of Novel Carbohydrate-Active Enzymes.

    PubMed

    Ufarté, Lisa; Bozonnet, Sophie; Laville, Elisabeth; Cecchini, Davide A; Pizzut-Serin, Sandra; Jacquiod, Samuel; Demanèche, Sandrine; Simonet, Pascal; Franqueville, Laure; Veronese, Gabrielle Potocki

    2016-01-01

    Activity-based metagenomics is one of the most efficient approaches to boost the discovery of novel biocatalysts from the huge reservoir of uncultivated bacteria. In this chapter, we describe a highly generic procedure of metagenomic library construction and high-throughput screening for carbohydrate-active enzymes. Applicable to any bacterial ecosystem, it enables the swift identification of functional enzymes that are highly efficient, alone or acting in synergy, to break down polysaccharides and oligosaccharides.

  2. Mark-resight abundance estimation under incomplete identification of marked individuals

    USGS Publications Warehouse

    McClintock, Brett T.; Hill, Jason M.; Fritz, Lowell; Chumbley, Kathryn; Luxa, Katie; Diefenbach, Duane R.

    2014-01-01

    Often less expensive and less invasive than conventional mark–recapture, so-called 'mark-resight' methods are popular in the estimation of population abundance. These methods are most often applied when a subset of the population of interest is marked (naturally or artificially), and non-invasive sighting data can be simultaneously collected for both marked and unmarked individuals. However, it can often be difficult to identify marked individuals with certainty during resighting surveys, and incomplete identification of marked individuals is potentially a major source of bias in mark-resight abundance estimators. Previously proposed solutions are ad hoc and will tend to underperform unless marked individual identification rates are relatively high (>90%) or individual sighting heterogeneity is negligible.Based on a complete data likelihood, we present an approach that properly accounts for uncertainty in marked individual detection histories when incomplete identifications occur. The models allow for individual heterogeneity in detection, sampling with (e.g. Poisson) or without (e.g. Bernoulli) replacement, and an unknown number of marked individuals. Using a custom Markov chain Monte Carlo algorithm to facilitate Bayesian inference, we demonstrate these models using two example data sets and investigate their properties via simulation experiments.We estimate abundance for grassland sparrow populations in Pennsylvania, USA when sampling was conducted with replacement and the number of marked individuals was either known or unknown. To increase marked individual identification probabilities, extensive territory mapping was used to assign incomplete identifications to individuals based on location. Despite marked individual identification probabilities as low as 67% in the absence of this territorial mapping procedure, we generally found little return (or need) for this time-consuming investment when using our proposed approach. We also estimate rookery abundance from Alaskan Steller sea lion counts when sampling was conducted without replacement, the number of marked individuals was unknown, and individual heterogeneity was suspected as non-negligible.In terms of estimator performance, our simulation experiments and examples demonstrated advantages of our proposed approach over previous methods, particularly when marked individual identification probabilities are low and individual heterogeneity levels are high. Our methodology can also reduce field effort requirements for marked individual identification, thus, allowing potential investment into additional marking events or resighting surveys.

  3. Developing Programs for the Gifted and Talented. 1985 Digest.

    ERIC Educational Resources Information Center

    Shrum, Judith L.

    Program development in gifted and talented education is the focus of the digest. Elements of a successful program, including statements of support for differential education, staff orientation, and multiple appropriate screening and identification procedures are listed. Beginning steps for planning committees are noted. Identification approaches…

  4. 49 CFR Appendix D to Part 236 - Independent Review of Verification and Validation

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... standards. (f) The reviewer shall analyze all Fault Tree Analyses (FTA), Failure Mode and Effects... for each product vulnerability cited by the reviewer; (4) Identification of any documentation or... not properly followed; (6) Identification of the software verification and validation procedures, as...

  5. Multiple Confidence Estimates as Indices of Eyewitness Memory

    ERIC Educational Resources Information Center

    Sauer, James D.; Brewer, Neil; Weber, Nathan

    2008-01-01

    Eyewitness identification decisions are vulnerable to various influences on witnesses' decision criteria that contribute to false identifications of innocent suspects and failures to choose perpetrators. An alternative procedure using confidence estimates to assess the degree of match between novel and previously viewed faces was investigated.…

  6. 18 CFR 1314.8 - Identification of accounts.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 18 Conservation of Power and Water Resources 2 2011-04-01 2011-04-01 false Identification of accounts. 1314.8 Section 1314.8 Conservation of Power and Water Resources TENNESSEE VALLEY AUTHORITY BOOK-ENTRY PROCEDURES FOR TVA POWER SECURITIES ISSUED THROUGH THE FEDERAL RESERVE BANKS § 1314.8...

  7. Comorbidity of Conduct Problems and ADHD: Identification of "Fledgling Psychopaths".

    ERIC Educational Resources Information Center

    Gresham, Frank M.; Lane, Kathleen L.; Lambros, Katina M.

    2000-01-01

    This article reviews the characteristics of children who exhibit a behavior pattern characterized by hyperactivity-impulsivity-inattention coupled with conduct problems such as fighting, stealing, truancy, noncompliance, and arguing. Procedures for early identification of these so-called "fledgling psychopaths" are described and discussed.…

  8. 33 CFR 337.9 - Identification and use of disposal areas.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ...) evaluation and environmental assessment or environmental impact statement should also address long-term... ARMY, DEPARTMENT OF DEFENSE PRACTICE AND PROCEDURE § 337.9 Identification and use of disposal areas. (a... life of existing disposal areas. Requests for water quality certification and/or coastal zone...

  9. Strain field reconstruction on composite spars based on the identification of equivalent load conditions

    NASA Astrophysics Data System (ADS)

    Airoldi, A.; Marelli, L.; Bettini, P.; Sala, G.; Apicella, A.

    2017-04-01

    Technologies based on optical fibers provide the possibility of installing relatively dense networks of sensors that can perform effective strain sensing functions during the operational life of structures. A contemporary trend is the increasing adoption of composite materials in aerospace constructions, which leads to structural architectures made of large monolithic elements. The paper is aimed at showing the feasibility of a detailed reconstruction of the strain field in a composite spar, which is based on the development of reference finite element models and the identification of load modes, consisting of a parameterized set of forces. The procedure is described and assessed in ideal conditions. Thereafter, a surrogate model is used to obtain realistic representation of the data acquired by the strain sensing system, so that the developed procedure is evaluated considering local effects due to the introduction of loads, significant modelling discrepancy in the development of the reference model and the presence of measurement noise. Results show that the method can obtain a robust and quite detailed reconstruction of strain fields, even at the level of local distributions, of the internal forces in the spars and of the displacements, by identifying an equivalent set of load parameters. Finally, the trade-off between the number of sensor and the accuracy, and the optimal position of the sensors for a given maximum number of sensors is evaluated by performing a multi-objective optimization, thus showing that even a relative dense network of externally applied sensors can be used to achieve good quality results.

  10. From pull-down data to protein interaction networks and complexes with biological relevance.

    PubMed

    Zhang, Bing; Park, Byung-Hoon; Karpinets, Tatiana; Samatova, Nagiza F

    2008-04-01

    Recent improvements in high-throughput Mass Spectrometry (MS) technology have expedited genome-wide discovery of protein-protein interactions by providing a capability of detecting protein complexes in a physiological setting. Computational inference of protein interaction networks and protein complexes from MS data are challenging. Advances are required in developing robust and seamlessly integrated procedures for assessment of protein-protein interaction affinities, mathematical representation of protein interaction networks, discovery of protein complexes and evaluation of their biological relevance. A multi-step but easy-to-follow framework for identifying protein complexes from MS pull-down data is introduced. It assesses interaction affinity between two proteins based on similarity of their co-purification patterns derived from MS data. It constructs a protein interaction network by adopting a knowledge-guided threshold selection method. Based on the network, it identifies protein complexes and infers their core components using a graph-theoretical approach. It deploys a statistical evaluation procedure to assess biological relevance of each found complex. On Saccharomyces cerevisiae pull-down data, the framework outperformed other more complicated schemes by at least 10% in F(1)-measure and identified 610 protein complexes with high-functional homogeneity based on the enrichment in Gene Ontology (GO) annotation. Manual examination of the complexes brought forward the hypotheses on cause of false identifications. Namely, co-purification of different protein complexes as mediated by a common non-protein molecule, such as DNA, might be a source of false positives. Protein identification bias in pull-down technology, such as the hydrophilic bias could result in false negatives.

  11. A volumetric pulmonary CT segmentation method with applications in emphysema assessment

    NASA Astrophysics Data System (ADS)

    Silva, José Silvestre; Silva, Augusto; Santos, Beatriz S.

    2006-03-01

    A segmentation method is a mandatory pre-processing step in many automated or semi-automated analysis tasks such as region identification and densitometric analysis, or even for 3D visualization purposes. In this work we present a fully automated volumetric pulmonary segmentation algorithm based on intensity discrimination and morphologic procedures. Our method first identifies the trachea as well as primary bronchi and then the pulmonary region is identified by applying a threshold and morphologic operations. When both lungs are in contact, additional procedures are performed to obtain two separated lung volumes. To evaluate the performance of the method, we compared contours extracted from 3D lung surfaces with reference contours, using several figures of merit. Results show that the worst case generally occurs at the middle sections of high resolution CT exams, due the presence of aerial and vascular structures. Nevertheless, the average error is inferior to the average error associated with radiologist inter-observer variability, which suggests that our method produces lung contours similar to those drawn by radiologists. The information created by our segmentation algorithm is used by an identification and representation method in pulmonary emphysema that also classifies emphysema according to its severity degree. Two clinically proved thresholds are applied which identify regions with severe emphysema, and with highly severe emphysema. Based on this thresholding strategy, an application for volumetric emphysema assessment was developed offering new display paradigms concerning the visualization of classification results. This framework is easily extendable to accommodate other classifiers namely those related with texture based segmentation as it is often the case with interstitial diseases.

  12. Evaluation of Bacto TB hydrolysis reagent (Tween 80) for the identification of Branhamella catarrhalis.

    PubMed Central

    Weiner, M; Penha, P D

    1990-01-01

    An investigation of the hydrolysis of Tween 80 reagent by Branhamella catarrhalis and related organisms (Neisseria and Moraxella species) revealed that only B. catarrhalis gave a positive result. A total of 226 strains, including reference organisms and clinical isolates, were studied. B. catarrhalis changed the color of the reagent from amber to pink-red after overnight incubation. We recommend this simple and cost-effective test as an alternative procedure to DNase testing or tributyrin hydrolysis or as a supplemental procedure for the identification of B. catarrhalis in clinical specimens. PMID:2105335

  13. Systematic toxicological analysis: computer-assisted identification of poisons in biological materials.

    PubMed

    Stimpfl, Th; Demuth, W; Varmuza, K; Vycudilik, W

    2003-06-05

    A new software was developed to improve the chances for identification of a "general unknown" in complex biological materials. To achieve this goal, the total ion current chromatogram was simplified by filtering the acquired mass spectra via an automated subtraction procedure, which removed mass spectra originating from the sample matrix, as well as interfering substances from the extraction procedure. It could be shown that this tool emphasizes mass spectra of exceptional compounds, and therefore provides the forensic toxicologist with further evidence-even in cases where mass spectral data of the unknown compound are not available in "standard" spectral libraries.

  14. SWI 1.10 Testing Process

    NASA Technical Reports Server (NTRS)

    Stokes, LeBarian

    2009-01-01

    This procedure establishes a system for performing testing in the Six-Degree-Of-Freedom Dynamic Test System (SDTS). Testing includes development and verification testing of customer supplied Test Articles (TAs) and other testing requirements, as requested. This procedure applies to all SDTS testing operations and equipment. The procedure provides an overview of testing performed in the SDTS including test identification requirements, test planning and procedure development, test and performance inspection, test data analysis, and test report generation.

  15. Tongue base reduction with hyoepiglottoplasty: a treatment for severe obstructive sleep apnea.

    PubMed

    Chabolle, F; Wagner, I; Blumen, M B; Séquert, C; Fleury, B; De Dieuleveult, T

    1999-08-01

    To describe a surgical procedure for the treatment of severe obstructive sleep apnea syndrome (OSAS), the procedure's indications, and its results. A retrospective study of 10 male patients with OSAS treated by tongue base reduction with hyoepiglottoplasty (TBRHE) at the Foch Hospital (Suresnes, France) between 1994 and 1997. Patients had a mean body mass index (BMI) of 32 kg/m2, a mean respiratory disturbance index (RDI) of 70 events/h, and a mean minimal oxygen saturation of 78%. They had refused positive airway pressure therapy or wished to discontinue it. Subtotal tongue base reduction preceded by lingual neurovascular bundle identification and derouting, epiglottal verticalization, mouth floor horizontalization, and hyoid bone repositioning was performed, associated in some cases to uvulopalatopharyngoplasty (UPPP). Indications were based on a site-related obstruction, on the absence of craniofacial deficiencies, and on the presence of hyolingual abnormalities determined by cephalometry and magnetic resonance imaging. TBRHE associated to UPPP in most cases had an 80% success rate, based on a postoperative RDI below 20 events/h and a reduction of the preoperative RDI of more than 50%. Snoring and excessive daytime sleepiness decreased or disappeared, respectively, in 100% and 90% of the cases. No neurovascular complications occurred. TBRHE is a safe procedure for the neurovascular bundle. Associated to a pharyngotomy, it is an effective treatment for severe OSAS attributable to tongue base obstruction. These results require confirmation in a larger series of patients.

  16. Anti-Exposure Technology Identification for Mission Specific Operational Requirements

    DTIC Science & Technology

    1981-08-08

    APPENDIX A- ANTHROPOMETRY MEASUREMENT PROCEDURES A-1 APPENDIX B - MOBILITY AND REACH MEASUREMENT PROCEDURES B-1 APPENDIX C - MOBILITY REDUCTION DATA...Anti-Exposure Configurations Studied 12 IV Garments/Flight Gear Worn With Each Configuration and Donning Sequence 18 V Subject Anthropometry (% ILE...body anthropometry . The following measurements were made according to the procedures described in Appendix A. Weight Stature (Height) Crotch Height

  17. Environmentally Friendly Procedure Based on Supercritical Fluid Chromatography and Tandem Mass Spectrometry Molecular Networking for the Discovery of Potent Antiviral Compounds from Euphorbia semiperfoliata.

    PubMed

    Nothias, Louis-Félix; Boutet-Mercey, Stéphanie; Cachet, Xavier; De La Torre, Erick; Laboureur, Laurent; Gallard, Jean-François; Retailleau, Pascal; Brunelle, Alain; Dorrestein, Pieter C; Costa, Jean; Bedoya, Luis M; Roussi, Fanny; Leyssen, Pieter; Alcami, José; Paolini, Julien; Litaudon, Marc; Touboul, David

    2017-10-27

    A supercritical fluid chromatography-based targeted purification procedure using tandem mass spectrometry and molecular networking was developed to analyze, annotate, and isolate secondary metabolites from complex plant extract mixture. This approach was applied for the targeted isolation of new antiviral diterpene esters from Euphorbia semiperfoliata whole plant extract. The analysis of bioactive fractions revealed that unknown diterpene esters, including jatrophane esters and phorbol esters, were present in the samples. The purification procedure using semipreparative supercritical fluid chromatography led to the isolation and identification of two new jatrophane esters (13 and 14) and one known (15) and three new 4-deoxyphorbol esters (16-18). The structure and absolute configuration of compound 16 were confirmed by X-ray crystallography. This compound was found to display antiviral activity against Chikungunya virus (EC 50 = 0.45 μM), while compound 15 proved to be a potent and selective inhibitor of HIV-1 replication in a recombinant virus assay (EC 50 = 13 nM). This study showed that a supercritical fluid chromatography-based protocol and molecular networking can facilitate and accelerate the discovery of bioactive small molecules by targeting molecules of interest, while minimizing the use of toxic solvents.

  18. Combining Rosetta with molecular dynamics (MD): A benchmark of the MD-based ensemble protein design.

    PubMed

    Ludwiczak, Jan; Jarmula, Adam; Dunin-Horkawicz, Stanislaw

    2018-07-01

    Computational protein design is a set of procedures for computing amino acid sequences that will fold into a specified structure. Rosetta Design, a commonly used software for protein design, allows for the effective identification of sequences compatible with a given backbone structure, while molecular dynamics (MD) simulations can thoroughly sample near-native conformations. We benchmarked a procedure in which Rosetta design is started on MD-derived structural ensembles and showed that such a combined approach generates 20-30% more diverse sequences than currently available methods with only a slight increase in computation time. Importantly, the increase in diversity is achieved without a loss in the quality of the designed sequences assessed by their resemblance to natural sequences. We demonstrate that the MD-based procedure is also applicable to de novo design tasks started from backbone structures without any sequence information. In addition, we implemented a protocol that can be used to assess the stability of designed models and to select the best candidates for experimental validation. In sum our results demonstrate that the MD ensemble-based flexible backbone design can be a viable method for protein design, especially for tasks that require a large pool of diverse sequences. Copyright © 2018 Elsevier Inc. All rights reserved.

  19. Identification of (antioxidative) plants in herbal pharmaceutical preparations and dietary supplements.

    PubMed

    Deconinck, Eric; Custers, Deborah; De Beer, Jacques Omer

    2015-01-01

    The standard procedures for the identification, authentication, and quality control of medicinal plants and herbs are nowadays limited to pure herbal products. No guidelines or procedures, describing the detection or identification of a targeted plant or herb in pharmaceutical preparations or dietary supplements, can be found. In these products the targeted plant is often present together with other components of herbal or synthetic origin. This chapter describes a strategy for the fast development of a chromatographic fingerprint approach that allows the identification of a targeted plant in herbal preparations and dietary supplements. The strategy consists of a standard chromatographic gradient that is tested for the targeted plant with different extraction solvents and different mobile phases. From the results obtained, the optimal fingerprint is selected. Subsequently the samples are analyzed according to the selected methodological parameters, and the obtained fingerprints can be compared with the one obtained for the pure herbal product or a standard preparation. Calculation of the dissimilarity between these fingerprints will result in a probability of presence of the targeted plant. Optionally mass spectrometry can be used to improve specificity, to confirm identification, or to identify molecules with a potential medicinal or antioxidant activity.

  20. The new laparoscopic proctocolectomy training (in soft cadaver).

    PubMed

    Pattana-arun, Jirawat; Udomsawaengsup, Suthep; Sahakitrungruang, Chucheep; Tansatit, Tanvaa; Tantiphlachiva, Kasaya; Rojanasakul, Arun

    2005-09-01

    The purpose of the present study was to evaluate the quality of preservation (tissue plane, named vessels identification, consistency of colon and rectum), quality of performing procedures, difficulties and problems and finally the satisfaction of surgeons in laparoscopic proctocolectomy in soft cadaver. Colorectal Division, Department of Surgery and Surgical Training Center Department of Anatomy, Faculty of Medicine, Chulalongkorn University. Prospective descriptive study 10 soft cadavers were scheduled for laparoscopic proctocolectomy. The procedures (colon-rectum mobilization and named vessels identification) were performed by 14 experienced surgeons (8 colorectal surgeons) and assisted by surgical residents. The quality of preservation, successfulness and the satisfaction in performing the procedures were recorded using questionnaires for evaluation. The preservation was very good in every aspect especially tissue plane between colon, mesocolon and retroperitoneum which was clearly dissected, same asfasciapropria of rectum. The named vessels and the tissue consistency were very well preserved and tolerated to laparoscopic equipment handling. The surgeons were satisfied with the tissue handling and dissections. There were two difficulties, the first was air leakage but simply corrected with purse string suture and the second was unflavored smell which was not concerned. Laparoscopic proctocolectomy could be completely performed in soft cadaver. Laparoscopic proctocolectomy could be performed in soft cadavers with great satisfaction. Repeated practice is possible, so the surgeons can gain their experiences outside the operating theatre. This success may shorten the learning curve and may be the new era in cadaver-based training.

  1. Radiofrequency-Based Identification Medical Device: An Evaluable Solution for Surgical Sponge Retrieval?

    PubMed

    Lazzaro, Alessandra; Corona, Arianna; Iezzi, Luca; Quaresima, Silvia; Armisi, Luca; Piccolo, Ilaria; Medaglia, Carlo Maria; Sbrenni, Sergio; Sileri, Pierpaolo; Rosato, Nicola; Gaspari, Achille Lucio; Di Lorenzo, Nicola

    2017-06-01

    A retained surgical item in patients (gossypiboma) is a persisting problem, despite consistent improvements and existing guidelines in counting instruments and sponges. Previous experiences with radiofrequency identification technology (RFID) tracking sponges show that it could represent an innovation, in order to reduce the criticism and increase the effectiveness during surgical procedures. We present an automated system that allows reduction of errors and improves safety in the operating room. The system consists of 3 antennas, surgical sponges containing RFID tags, and dedicated software applications, with Wi-Fi real-time communication between devices. The first antenna provides the initial count of gauzes; the second a real-time counting during surgery, including the sponges thrown into the kick-bucket; and the third can be used in the event of uneven sponge count. The software allows management at all stages of the process. In vitro and in vivo tests were performed: the system provided excellent results in detecting sponges in patients' body. Hundred percent retained sponges were detected correctly, even when they were overlapped. No false positive or false negative was recorded. The counting procedure turned out to be more streamlined and efficient and it could save time in a standard procedure. The RFID system for sponge tracking was shown to be experimentally a reliable and feasible method to track sponges with a full detection accuracy in the operating room. The results indicate the system to be safe and effective with acceptable cost-effective parameters.

  2. Fiber sensor assisted in-vivo needle guidance for minimally invasive procedures (Conference Presentation)

    NASA Astrophysics Data System (ADS)

    Baghdadchi, Saharnaz; Chao, Cherng; Esener, Sadik; Mattrey, Robert F.; Eghtedari, Mohammad A.

    2017-02-01

    Image-guided procedures are performed frequently by radiologists to insert a catheter within a target vessel or lumen or to perform biopsy of a lesion. For instance, an interventional radiologist uses fluoroscopy during percutaneous biliary drainage procedure (a procedure during which a catheter is inserted through the skin to drain the bile from liver) to identify the location of the needle tip within liver parenchyma, hepatic blood vessel or bile duct. However, the identification of the target organ under fluoroscopy exposes the patient to x-ray irradiation, which may be significant if the time of procedure is prolonged. We have designed a fiber core needle system that may help the radiologist identify the location of the needle tip in real time without exposing the patient to x-ray. Our needle system transmits a low power modulated light into the tissue through a fiber cable embedded in the needle and detects the backscattered light using another fiber inside the needle. We were able to successfully distinguish the location of our prototype needle tip inside a cow liver phantom to identify if the needle tip was within liver parenchyma, liver vessels, or in the bile duct based on the recorded backscattered light.

  3. Dissemination of Periodontal Pathogens in the Bloodstream after Periodontal Procedures: A Systematic Review

    PubMed Central

    Horliana, Anna Carolina Ratto Tempestini; Chambrone, Leandro; Foz, Adriana Moura; Artese, Hilana Paula Carillo; Rabelo, Mariana de Sousa; Pannuti, Cláudio Mendes; Romito, Giuseppe Alexandre

    2014-01-01

    Background To date, there is no compilation of evidence-based information associating bacteremia and periodontal procedures. This systematic review aims to assess magnitude, duration, prevalence and nature of bacteremia caused by periodontal procedures. Study Design Systematic Review Types of Studies Reviewed MEDLINE, EMBASE and LILACS databases were searched in duplicate through August, 2013 without language restriction. Observational studies were included if blood samples were collected before, during or after periodontal procedures of patients with periodontitis. The methodological quality was assessed in duplicate using the modified Newcastle-Ottawa scale (NOS). Results Search strategy identified 509 potentially eligible articles and nine were included. Only four studies demonstrated high methodological quality, whereas five were of medium or low methodological quality. The study characteristics were considered too heterogeneous to conduct a meta-analysis. Among 219 analyzed patients, 106 (49.4%) had positive bacteremia. More frequent bacteria were S. viridans, A. actinomycetemcomitans P. gingivalis, M. micros and species Streptococcus and Actinomyces, although identification methods of microbiologic assays were different among studies. Clinical Implications Although half of the patients presented positive bacteremia after periodontal procedures, accurate results regarding the magnitude, duration and nature of bacteremia could not be confidentially assessed. PMID:24870125

  4. Behavioral Assessment Instruments, Techniques, and Procedures: Summary and Annotated Bibliography.

    ERIC Educational Resources Information Center

    Shorkey, Clayton T.; Williams, Harry

    This annotated bibliography cites 223 articles related to behavioral assessment reported in 18 professional journals between January 1960 and Spring 1976. A summary and a reference grouping of the articles are included to allow for identification of articles related to (1) electromechanical devices used in identification, measurement, and storage…

  5. Metaphor Identification as a Research Method for the Study of Career

    ERIC Educational Resources Information Center

    Creed, Allison; McIlveen, Peter

    2018-01-01

    In this paper, we present the Metaphor Identification Procedure Vrije Universiteit (MIPVU) for the reliable detection of metaphoric language that may be interpreted using extant vocational psychology theory. Metaphors are ubiquitous in communication and carry influential meaning for people. To illustrate MIPVU, we use transcripts of interviews…

  6. 24 CFR 203.35 - Disclosure and verification of Social Security and Employer Identification Numbers.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... Social Security and Employer Identification Numbers. 203.35 Section 203.35 Housing and Urban Development... Requirements and Underwriting Procedures Eligible Mortgagors § 203.35 Disclosure and verification of Social... mortgagor must meet the requirements for the disclosure and verification of Social Security and Employer...

  7. 40 CFR 255.1 - Scope and purpose.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... waste management services pursuant to section 4002(a) of the Solid Waste Disposal Act, as amended by the... guidelines are applicable to policies, procedures, and criteria for the identification of those areas which... identification of which functions will be carried out by which agencies pursuant to section 4006 of the Act. (b...

  8. 40 CFR 255.1 - Scope and purpose.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... waste management services pursuant to section 4002(a) of the Solid Waste Disposal Act, as amended by the... guidelines are applicable to policies, procedures, and criteria for the identification of those areas which... identification of which functions will be carried out by which agencies pursuant to section 4006 of the Act. (b...

  9. Technical Assistance Resource Guide for Teachers Educating Students with: Attention Deficit Hyperactivity Disorder.

    ERIC Educational Resources Information Center

    Idaho State Dept. of Education. Special Education Section.

    Designed for teachers, this guide provides recommendations regarding identification, interventions, and follow-through procedures for working with students with attention deficit hyperactivity disorder (ADHD) in a classroom setting. Chapter 1 discusses the identification of children with ADHD and includes information on the definition of ADHD,…

  10. Security Procedures for Golden Laboratories and Administration Offices |

    Science.gov Websites

    photo identification (for example driver's license, passport, or military ID). Upon arrival to NREL's and should park in the Research Support Facility (RSF) Visitor Parking Lot. Visitors will then enter ) window. Visitors need to be prepared to show government-issued photo identification (for example driver's

  11. 78 FR 3338 - High Seas Driftnet Fishing Moratorium Protection Act; Identification and Certification Procedures...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-01-16

    .... 1103210208-2676-02] RIN 0648-BA89 High Seas Driftnet Fishing Moratorium Protection Act; Identification and... Conservation Act, which amended the High Seas Driftnet Fishing Moratorium Protection Act (Moratorium Protection... protected living marine resources (PLMRs) pursuant to the High Seas Driftnet Fishing Moratorium Protection...

  12. Problem Identification Survey Handbook for Parish Rural Development Committees.

    ERIC Educational Resources Information Center

    Koch, Kenneth A.

    The Task Force on Resource Development of the Cooperative Extension Service, Louisiana State University, decided to utilize the leader identification technique as a means of assisting the parish rural development committees in the rural development process. The technique is a procedure to stimulate effective citizen participation to identify…

  13. Identification of 180 million years old, probably unchanged melanine

    NASA Technical Reports Server (NTRS)

    Beyermann, K.; Hasenmaier, D.

    1977-01-01

    The comparison of the infrared spectra of recent sepia melanine and of the content of the ink sac of fossilized cuttlefish indicates that the 180 million years old substance is unchanged melanine. Both substances behave identically on heating. Other procedures for identification of melanine are surveyed critically.

  14. 78 FR 63017 - Exhaust Emissions Standards for New Aircraft Gas Turbine Engines and Identification Plate for...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-10-23

    ... Aircraft Gas Turbine Engines and Identification Plate for Aircraft Engines AGENCY: Federal Aviation... (NO X ), compliance flexibilities, and other regulatory requirements for aircraft turbofan or turbojet... adopting the gas turbine engine test procedures of the International Civil Aviation Organization (ICAO...

  15. 47 CFR 3.22 - Number of accounting authority identification codes per applicant.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... AUTHORIZATION AND ADMINISTRATION OF ACCOUNTING AUTHORITIES IN MARITIME AND MARITIME MOBILE-SATELLITE RADIO SERVICES Application Procedures § 3.22 Number of accounting authority identification codes per applicant... assign U.S. AAICs for entities settling accounts of U.S. licensed vessels in the maritime mobile and...

  16. Three Thousand Years of Talent Searching.

    ERIC Educational Resources Information Center

    Wing, Cliff W., Jr.

    An overview is presented of current issues in the assessment of gifted and talented persons, with an emphasis on generalizable and universal talents and the operational methods used in their identification. The Chinese were seeking out talented individuals through formal identification procedures as early as 2200 BC. In the Western world, the use…

  17. 24 CFR 203.35 - Disclosure and verification of Social Security and Employer Identification Numbers.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... Social Security and Employer Identification Numbers. 203.35 Section 203.35 Housing and Urban Development... Requirements and Underwriting Procedures Eligible Mortgagors § 203.35 Disclosure and verification of Social... mortgagor must meet the requirements for the disclosure and verification of Social Security and Employer...

  18. 24 CFR 203.35 - Disclosure and verification of Social Security and Employer Identification Numbers.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... Social Security and Employer Identification Numbers. 203.35 Section 203.35 Housing and Urban Development... Requirements and Underwriting Procedures Eligible Mortgagors § 203.35 Disclosure and verification of Social... mortgagor must meet the requirements for the disclosure and verification of Social Security and Employer...

  19. 24 CFR 203.35 - Disclosure and verification of Social Security and Employer Identification Numbers.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... Social Security and Employer Identification Numbers. 203.35 Section 203.35 Housing and Urban Development... Requirements and Underwriting Procedures Eligible Mortgagors § 203.35 Disclosure and verification of Social... mortgagor must meet the requirements for the disclosure and verification of Social Security and Employer...

  20. 24 CFR 203.35 - Disclosure and verification of Social Security and Employer Identification Numbers.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... Social Security and Employer Identification Numbers. 203.35 Section 203.35 Housing and Urban Development... Requirements and Underwriting Procedures Eligible Mortgagors § 203.35 Disclosure and verification of Social... mortgagor must meet the requirements for the disclosure and verification of Social Security and Employer...

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