Molecular Genetics of Root Thigmoresponsiveness in Arabidopsis thaliana

NASA Technical Reports Server (NTRS)

Masson, Patrick H.

2002-01-01

The molecular mechanisms that allow plant roots to use gravity and touch as growth guides are investigated. We are using a molecular genetic strategy in Arabidopsis thaliana to study these processes. When Arabidopsis thaliana seedlings grow on tilted hard-agar surfaces, their roots develop a wavy pattern of growth which appears to derive from a succession of left-handed and right-handed circumnutation-like processes triggered by gravity and touch stimulation (Okada and Shimura, 1990; Rutherford et al., 1998; Rutherford and Masson, 1996). Interestingly, mutations that affect root waving on tilted hard-agar surfaces can be identified and characterized. Some of these mutations affect root gravitropism, while others appear to be responsible for the production of abnormal waves (no waves, compressed or square waves, coils) without affecting gravitropism. The specific objectives of this project were to functionally characterize two genes (WVD2 and WVD6) which are required for root waving on tilted agar surfaces, but not for root gravitropism. Specific objectives included a physiological and cytological analysis of the mutants, and molecular cloning and characterization of the corresponding genes. As summarized in this paper, we have reached these objectives. We have also identified and partially characterized other mutations that affect root skewing on hard-agar surfaces (sku5-1 and ago1), and have completed our work on the root-wave phenotype associated with mutations in genes of the tryptophan biosynthesis pathway (Lynn et al., 1999; Rutherford et al., 1998; Sedbrook et al., 2000, 2002). We briefly describe our progress on the cloning and characterization of WVD6, WVD2 and SKU5, and provide a list of papers (published, or in preparation) that derived from this grant. We also discuss the biological implications of our findings, with special emphasis on the analysis of WVD2.

Characterization of Debris from the DebriSat Hypervelocity Test

NASA Technical Reports Server (NTRS)

Rivero, M.; Kleespies, J.; Patankar, K.; Fitz-Coy, N.; Liou, J.-C.; Sorge, M.; Huynh, T.; Opiela, J.; Krisko, P.; Cowardin, H.

2015-01-01

The DebriSat project is an effort by NASA and the DoD to update the standard break-up model for objects in orbit. The DebriSat object, a 56 kg representative LEO satellite, was subjected to a hypervelocity impact in April 2014. For the hypervelocity test, the representative satellite was suspended within a "soft-catch" arena formed by polyurethane foam panels to minimize the interactions between the debris generated from the hypervelocity impact and the metallic walls of the test chamber. After the impact, the foam panels and debris not caught by the panels were collected and shipped to the University of Florida where the project has now advanced to the debris characterization stage. The characterization effort has been divided into debris collection, measurement, and cataloguing. Debris collection and cataloguing involves the retrieval of debris from the foam panels and cataloguing the debris in a database. Debris collection is a three-step process: removal of loose debris fragments from the surface of the foam panels; X-ray imaging to identify/locate debris fragments embedded within the foam panel; extraction of the embedded debris fragments identified during the X-ray imaging process. As debris fragments are collected, they are catalogued into a database specifically designed for this project. Measurement involves determination of size, mass, shape, material, and other physical properties and well as images of the fragment. Cataloguing involves a assigning a unique identifier for each fragment along with the characterization information.

Compatibility Grab Sampling and Analysis Plan for FY 2000

DOE Office of Scientific and Technical Information (OSTI.GOV)

SASAKI, L.M.

1999-12-29

This sampling and analysis plan (SAP) identifies characterization objectives pertaining to sample collection, laboratory analytical evaluation, and reporting requirements for grab samples obtained to address waste compatibility. It is written in accordance with requirements identified in Data Quality Objectives for Tank Farms Waste Compatibility Program (Mulkey et al. 1999) and Tank Farm Waste Transfer Compatibility Program (Fowler 1999). In addition to analyses to support Compatibility, the Waste Feed Delivery program has requested that tank samples obtained for Compatibility also be analyzed to confirm the high-level waste and/or low-activity waste envelope(s) for the tank waste (Baldwin 1999). The analytical requirements tomore » confirm waste envelopes are identified in Data Quality Objectives for TWRS Privatization Phase I: Confirm Tank T is an Appropriate Feed Source for Low-Activity Waste Feed Batch X (Nguyen 1999a) and Data Quality Objectives for RPP Privatization Phase I: Confirm Tank T is an Appropriate Feed Source for High-Level Waste Feed Batch X (Nguyen 1999b).« less

Structural characterization of UHPC waffle bridge deck and connections.

DOT National Transportation Integrated Search

2014-07-01

The AASHTO strategic plan in 2005 for bridge engineering identified extending the service life of bridges and accelerating bridge : construction as two of the grand challenges in bridge engineering. These challenges have the objective of producing sa...

A NORMATIVE STUDY OF CHILDREN'S HOUSE-TREE-PERSON DRAWINGS.

ERIC Educational Resources Information Center

RAPPAPORT, SHELDON R.

THIS STUDY WAS THE FIRST PHASE OF A THREE-PART PROJECT WHOSE GOAL IS TO ESTABLISH VALID CRITERIA FOR IDENTIFYING THE HOUSE-TREE-PERSON (H-T-P) DRAWINGS OF NORMAL CHILDREN THROUGHOUT THE ELEMENTARY SCHOOL YEARS. THE SPECIFIC OBJECTIVES OF THIS STUDY WERE (1) TO IDENTIFY WHICH ITEMS OF THE H-T-P TEST CHARACTERIZE NORMAL DEVELOPMENT THROUGH GRADES 2,…

Categorization and Characterization of American Driving Conditions (Phase I)

DOT National Transportation Integrated Search

1978-11-01

The objectives of the study were: (1) to develop a multidimensional matrix as an analysis framework to classify travel of personal motor vehicles according to fuel consumption, (2) to identify and assess available information on travel and fuel consu...

Interrill soil erosion processes on steep slopes

USDA-ARS?s Scientific Manuscript database

To date interrill erosion processes and regimes are not fully understood. The objectives are to 1) identify the erosion regimes and limiting processes between detachment and transport on steep slopes, 2) characterize the interactive effects between rainfall intensity and flow depth on sediment trans...

Electro-optical characterization of GaAs solar cells

NASA Technical Reports Server (NTRS)

Olsen, Larry C.; Dunham, Glen; Addis, F. W.; Huber, Dan; Daling, Dave

1987-01-01

The electro-optical characterization of gallium arsenide p/n solar cells is discussed. The objective is to identify and understand basic mechanisms which limit the performance of high efficiency gallium arsenide solar cells. The approach involves conducting photoresponse and temperature dependent current-voltage measurements, and interpretation of the data in terms of theory to determine key device parameters. Depth concentration profiles are also utilized in formulating a model to explain device performance.

Exploring the Structure of the Distant Universe with MUSE Data Cubes

NASA Astrophysics Data System (ADS)

MacDougall, Mason; Christensen, Lise

2018-01-01

The mass distribution in intergalactic and circumgalactic space is not well known since it is difficult to characterize objects in the distant universe. An ideal tool for studying such distant structure is the Multi-Unit Spectroscopic Explorer (MUSE) of the Very Large Telescope array, which employs a wide field-of-view and a large spectral range to produce high spatial resolution datasets. Here we exploit the 2 spatial dimensions and 1 spectral dimension of a particular MUSE “data cube” to identify and characterize emission line sources near the line-of-sight to quasar PKS1937-101, which lies at a redshift of z=3.787. In particular, we search for galaxy companions to a z=3.572 Lyman-limit system measured in the quasar spectrum and find an associated Lyman-alpha emitter at z=3.556 with a projected distance of 30.2 kpc from the quasar line-of-sight. Through a combination of automated source extraction and manual investigation, we also identify 25 emission line galaxies and 1 other Lyman-alpha emitter in our field. The proximity of several of these objects to the quasar line-of-sight allows us to reliably identify absorption lines in the quasar spectrum that can be associated with observed emission lines with resolved fluxes. This will help characterize the metallicities and kinematics of galaxy halos and circumgalactic media in the early universe.

Spectroscopic confirmation of the low-latitude object FSR 1716 as an old globular cluster

NASA Astrophysics Data System (ADS)

Koch, Andreas; Kunder, Andrea; Wojno, Jennifer

2017-09-01

Star clusters are invaluable tracers of the Galactic components and the discovery and characterization of low-mass stellar systems can be used to appraise their prevailing disruption mechanisms and time scales. However, owing to significant foreground contamination, high extinction, and still uncharted interfaces of the underlying Milky Way components, objects at low Galactic latitudes are notoriously difficult to characterize. Here, we present the first spectroscopic campaign to identify the chemodynamical properties of the low-latitude star cluster FSR 1716. While its photometric age and distance are far from settled, the presence of RR Lyrae variables indicates a rather old cluster variety. Using medium-resolution (R 10 600) calcium triplet (CaT) spectroscopy obtained with the wide-field, multi-fiber AAOmega instrument, we identified six member candidates with a mean velocity of -30 km s-1 and a velocity dispersion of 2.5 ± 0.9 km s-1. The latter value implies a dynamic mass of 1.3 × 104M⊙, typical of a low-mass globular cluster. Combined with our derived CaT metallicity of -1.38 ± 0.20 dex, this object is finally confirmed as an old, metal-poor globular cluster.

Characterizing the WISE-selected heavily obscured quasar population with optical spectroscopy from the Southern African Large Telescope

NASA Astrophysics Data System (ADS)

Hviding, Raphael E.; Hickox, Ryan C.; Hainline, Kevin N.; Carroll, Christopher M.; DiPompeo, Michael A.; Yan, Wei; Jones, Mackenzie L.

2018-02-01

We present the results of an optical spectroscopic survey of 46 heavily obscured quasar candidates. Objects are selected using their mid-infrared (mid-IR) colours and magnitudes from the Wide-Field Infrared Survey Explorer (WISE) anzd their optical magnitudes from the Sloan Digital Sky Survey. Candidate Active Galactic Nuclei (AGNs) are selected to have mid-IR colours indicative of quasar activity and lie in a region of mid-IR colour space outside previously published X-ray based selection regions. We obtain optical spectra for our sample using the Robert Stobie Spectrograph on the Southern African Large Telescope. 30 objects (65 per cent) have identifiable emission lines, allowing for the determination of spectroscopic redshifts. Other than one object at z ˜ 2.6, candidates have moderate redshifts ranging from z = 0.1 to 0.8 with a median of 0.3. 21 (70 per cent) of our objects with identified redshift (46 per cent of the whole sample) are identified as AGNs through common optical diagnostics. We model the spectral energy distributions of our sample and found that all require a strong AGN component, with an average intrinsic AGN fraction at 8 μm of 0.91. Additionally, the fits require large extinction coefficients with an average E(B - V)AGN = 17.8 (average A(V)AGN = 53.4). By focusing on the area outside traditional mid-IR photometric cuts, we are able to capture and characterize a population of deeply buried quasars that were previously unattainable through X-ray surveys alone.

STORMWATER TREATMENT AT CRITICAL AREAS: THE MULTI-CHAMBERED TREATMENT TRAIN (MCTT)

EPA Science Inventory

Past studies have identified urban runoff as a major contributor to the degradation of many urban streams and rivers. The objective of this research was to characterize typical toxicant concentrations in stormwater, and investigate the effectiveness of treatment processes to con...

A fast 3-D object recognition algorithm for the vision system of a special-purpose dexterous manipulator

NASA Technical Reports Server (NTRS)

Hung, Stephen H. Y.

1989-01-01

A fast 3-D object recognition algorithm that can be used as a quick-look subsystem to the vision system for the Special-Purpose Dexterous Manipulator (SPDM) is described. Global features that can be easily computed from range data are used to characterize the images of a viewer-centered model of an object. This algorithm will speed up the processing by eliminating the low level processing whenever possible. It may identify the object, reject a set of bad data in the early stage, or create a better environment for a more powerful algorithm to carry the work further.

Nondestructive Concrete Characterization System

DTIC Science & Technology

2013-05-20

Army, locate steel reinforcing bars, and identify the presence of steel fiber reinforcement . The thickness of all sides of each concrete block was...concrete compressive strength within the accuracy required by the U.S. Army, locate steel reinforcing bars, and identify the presence of steel fiber ...tolerance of ±3 ksi. 3. Detect the presence of fiber reinforcement . 4. Locate and detect the presence and density (e.g. spacing) of metallic objects

Isolation and characterization of probiotics from dairies

PubMed Central

Haghshenas, Babak; Nami, Yousef; Almasi, Ali; Abdullah, Norhafizah; Radiah, Dayang; Rosli, Rozita; Barzegari, Abolfazl; Khosroushahi, Ahmad Yari

2017-01-01

Background and Objectives: Probiotics are live microorganisms, which show beneficial health effects on hosts once consumed in sufficient amounts. LAB group can be isolated and characterized from traditional dairy sources. This study aimed at isolating, identifying, and in vitro characterizing (low pH/high bile salt tolerance, antibacterial activity, and antibiotic susceptibility) LAB strains from traditional Iranian dairy products. Materials and Methods: Isolated strains were identified by Gram staining, catalase assay, and 3 molecular identification methods; namely, (GTG) 5-PCR fingerprinting, ARDRA, and 16S rDNA gene sequencing. Results: A total of 19 LAB strains belonging to 4 genera (Lactococcus, Leuconostoc, Lactobacillus and Enterococcus) were identified. Conclusion: The experiments revealed that L. plantarum 15HN, L. lactis subsp. cremoris 44L and E. mundtii 50H strains, which were isolated from shiraz, cheese and shiraz, respectively, displayed a desirable tolerance to low pH and high bile salts, favorable anti-pathogen activity, and acceptable antibiotic susceptibility; hence, they could be considered as novel probiotic candidates and applied in the food industry. PMID:29238459

Large truck crashes in Texas : a predictive approach for identifying those at higher risk

DOT National Transportation Integrated Search

2007-08-01

The objective of this research is to characterize large truck safety levels in Texas on the basis of driver, vehicle, cargo, and carrier traits while controlling for the effects of crash and operating environment conditions. Data to support this inve...

Characterization of alfalfa populations contrasting for root system architecture (RSA)

USDA-ARS?s Scientific Manuscript database

The root system architecture affects the capacity for nutrient and water uptake thus impacting biomass yield production and may contribute to the persistence of perennial plants. The objectives of this study were to phenotype the roots of three alfalfa populations and identify differences between di...

CHARACTERIZING SURFACE WATERS THAT MAY NOT REQUIRE FILTRATION

EPA Science Inventory

Field data from various utilities were studied with the object of identifying a set of characteristics of a surface water that might allow it to be successfully treated by disinfection alone, thus avoiding the need to filter. It was found possible to define water quality standard...

Identifying On-Orbit Test Targets for Space Fence Operational Testing

NASA Astrophysics Data System (ADS)

Pechkis, D.; Pacheco, N.; Botting, T.

2014-09-01

Space Fence will be an integrated system of two ground-based, S-band (2 to 4 GHz) phased-array radars located in Kwajalein and perhaps Western Australia [1]. Space Fence will cooperate with other Space Surveillance Network sensors to provide space object tracking and radar characterization data to support U.S. Strategic Command space object catalog maintenance and other space situational awareness needs. We present a rigorous statistical test design intended to test Space Fence to the letter of the program requirements as well as to characterize the system performance across the entire operational envelope. The design uses altitude, size, and inclination as independent factors in statistical tests of dependent variables (e.g., observation accuracy) linked to requirements. The analysis derives the type and number of necessary test targets. Comparing the resulting sample sizes with the number of currently known targets, we identify those areas where modelling and simulation methods are needed. Assuming hypothetical Kwajalein radar coverage and a conservative number of radar passes per object per day, we conclude that tests involving real-world space objects should take no more than 25 days to evaluate all operational requirements; almost 60 percent of the requirements can be tested in a single day and nearly 90 percent can be tested in one week or less. Reference: [1] L. Haines and P. Phu, Space Fence PDR Concept Development Phase, 2011 AMOS Conference Technical Papers.

Raman Laser Spectrometer (RLS) on-board data processing and compression

NASA Astrophysics Data System (ADS)

Diaz, C.; Lopez, G.; Hermosilla, I.; Catalá, A.; Rodriguez, J. A.; Perez, C.; Diaz, E.

2013-09-01

The Raman Laser Spectrometer (RLS) is one of the Pasteur Payload instruments, within the ESA's Aurora Exploration Programme, ExoMars mission. Particularly, the RLS scientific objectives are as follows: identify organic compound and search for life; identify the mineral products and indicators of biologic activities; characterize mineral phases produced by water-related processes; characterize igneous minerals and their alteration products; characterise water/geochemical environment as a function of depth in the shallow subsurface. The straightforward approach of operating the instrument would result in a vast amount of spectrum images. A flexible on-board data processing concept has been designed to accommodate scientific return to the sample nature and data downlink bandwidth.

A global survey of low-molecular weight carbohydrates in lentils

USDA-ARS?s Scientific Manuscript database

Lentils contain a range of low-molecular weight carbohydrates (LMWC); however, they have not been well characterized. The objectives of this study were to (1) determine the concentrations of LMWC in lentils grown in different environments and (2) identify any genetic and environmental effects on tho...

A global survey of low-molecular weight carbohydrates in lentils

USDA-ARS?s Scientific Manuscript database

Lentils contain a range of low-molecular weight carbohydrates (LMWC); however, those have not been well characterized. The objectives of this study were to (1) determine the concentrations of LMWC in lentils grown in six locations, and (2) identify any genetic and environmental effects on those LMWC...

Nuclear Security: Target Analysis

DOE Office of Scientific and Technical Information (OSTI.GOV)

Singh, Surinder Paul; Gibbs, Philip W.; Bultz, Garl A.

2014-03-01

This objectives of this session were to understand the basic steps of target identification; describe the SNRI targets in detail; characterize specific targets with more detail; prioritize targets based on guidance documents; understand the graded safeguards concept; identify roll up and understand why it is a concern; and recognize the category for different materials.

Identifying the Proteins that Mediate the Ionizing Radiation Resistance of Deinococcus Radiodurans R1

DOE Office of Scientific and Technical Information (OSTI.GOV)

Battista, John R

The primary objectives of this proposal was to define the subset of proteins required for the ionizing radiation (IR) resistance of Deinococcus radiodurans R1, characterize the activities of those proteins, and apply what was learned to problems of interest to the Department of Energy.

Alternative Environments for Basic Skills Development.

ERIC Educational Resources Information Center

Crowe, Michael R.; And Others

This study focused on the identification and description of environmental characteristics and their relationship to basic skills exposure. The objectives of the study were to identify the major factors that characterize environments in which learning is intended to occur, and to delineate patterns of co-exposure to basic skills and environmental…

Understanding erosion process using rare earth element tracers in a preformed interrill-rill system

USDA-ARS?s Scientific Manuscript database

Tracking sediment source and movement is essential to fully understanding soil erosion processes. The objectives of this study were to identify dominant erosion process and to characterize the effects of upslope interrill erosion on downslope interrill and rill erosion in a preformed interrill-rill ...

Pathways to Careers in Communication.

ERIC Educational Resources Information Center

Chesebro, James W.

his booklet serves the following objectives; (1) it provides an overview of the role of communication in the United States economy and labor force; (2) it characterizes developments in the discipline of communication; (3) it identifies the relationships between employment areas and communication training; (4) it offers trends and projections in…

Characterization of Magnetospheric Spacecraft Charging Environments Using the LANL Magnetospheric Plasma Analyzer Data Set

NASA Technical Reports Server (NTRS)

Hardage, Donna (Technical Monitor); Davis, V. A.; Mandell, M. J.; Thomsen, M. F.

2003-01-01

An improved specification of the plasma environment has been developed for use in modeling spacecraft charging. It was developed by statistically analyzing a large part of the LANL Magnetospheric Plasma Analyzer (MPA) data set for ion and electron spectral signature correlation with spacecraft charging, including anisotropies. The objective is to identify a relatively simple characterization of the full particle distributions that yield an accurate predication of the observed charging under a wide variety of conditions.

Quarterly Technical Progress Report

DOE Office of Scientific and Technical Information (OSTI.GOV)

Mary K. Banken

This project has identified all FDD oil reservoirs in Oklahoma; grouped those reservoirs into plays that have similar depositional origins; collected, organized, and analyzed all available data; conducted characterization and simulation studies on selected reservoirs in each plays; and implemented a technology transfer program targeted to the operators of FDD reservoirs. By fulfilling these objectives, the FDD project has had the goal of helping to sustain the life expectancy of existing wells and provide incentive for development and exploratory wells with the ultimate objective of increasing oil recovery.

In-Depth Characterization and Validation of Human Urine Metabolomes Reveal Novel Metabolic Signatures of Lower Urinary Tract Symptoms

NASA Astrophysics Data System (ADS)

Hao, Ling; Greer, Tyler; Page, David; Shi, Yatao; Vezina, Chad M.; Macoska, Jill A.; Marker, Paul C.; Bjorling, Dale E.; Bushman, Wade; Ricke, William A.; Li, Lingjun

2016-08-01

Lower urinary tract symptoms (LUTS) are a range of irritative or obstructive symptoms that commonly afflict aging population. The diagnosis is mostly based on patient-reported symptoms, and current medication often fails to completely eliminate these symptoms. There is a pressing need for objective non-invasive approaches to measure symptoms and understand disease mechanisms. We developed an in-depth workflow combining urine metabolomics analysis and machine learning bioinformatics to characterize metabolic alterations and support objective diagnosis of LUTS. Machine learning feature selection and statistical tests were combined to identify candidate biomarkers, which were statistically validated with leave-one-patient-out cross-validation and absolutely quantified by selected reaction monitoring assay. Receiver operating characteristic analysis showed highly-accurate prediction power of candidate biomarkers to stratify patients into disease or non-diseased categories. The key metabolites and pathways may be possibly correlated with smooth muscle tone changes, increased collagen content, and inflammation, which have been identified as potential contributors to urinary dysfunction in humans and rodents. Periurethral tissue staining revealed a significant increase in collagen content and tissue stiffness in men with LUTS. Together, our study provides the first characterization and validation of LUTS urinary metabolites and pathways to support the future development of a urine-based diagnostic test for LUTS.

Proteome Characterization of Leaves in Common Bean

PubMed Central

Robison, Faith M.; Heuberger, Adam L.; Brick, Mark A.; Prenni, Jessica E.

2015-01-01

Dry edible bean (Phaseolus vulgaris L.) is a globally relevant food crop. The bean genome was recently sequenced and annotated allowing for proteomics investigations aimed at characterization of leaf phenotypes important to agriculture. The objective of this study was to utilize a shotgun proteomics approach to characterize the leaf proteome and to identify protein abundance differences between two bean lines with known variation in their physiological resistance to biotic stresses. Overall, 640 proteins were confidently identified. Among these are proteins known to be involved in a variety of molecular functions including oxidoreductase activity, binding peroxidase activity, and hydrolase activity. Twenty nine proteins were found to significantly vary in abundance (p-value < 0.05) between the two bean lines, including proteins associated with biotic stress. To our knowledge, this work represents the first large scale shotgun proteomic analysis of beans and our results lay the groundwork for future studies designed to investigate the molecular mechanisms involved in pathogen resistance. PMID:28248269

Bayesian exploration for intelligent identification of textures.

PubMed

Fishel, Jeremy A; Loeb, Gerald E

2012-01-01

In order to endow robots with human-like abilities to characterize and identify objects, they must be provided with tactile sensors and intelligent algorithms to select, control, and interpret data from useful exploratory movements. Humans make informed decisions on the sequence of exploratory movements that would yield the most information for the task, depending on what the object may be and prior knowledge of what to expect from possible exploratory movements. This study is focused on texture discrimination, a subset of a much larger group of exploratory movements and percepts that humans use to discriminate, characterize, and identify objects. Using a testbed equipped with a biologically inspired tactile sensor (the BioTac), we produced sliding movements similar to those that humans make when exploring textures. Measurement of tactile vibrations and reaction forces when exploring textures were used to extract measures of textural properties inspired from psychophysical literature (traction, roughness, and fineness). Different combinations of normal force and velocity were identified to be useful for each of these three properties. A total of 117 textures were explored with these three movements to create a database of prior experience to use for identifying these same textures in future encounters. When exploring a texture, the discrimination algorithm adaptively selects the optimal movement to make and property to measure based on previous experience to differentiate the texture from a set of plausible candidates, a process we call Bayesian exploration. Performance of 99.6% in correctly discriminating pairs of similar textures was found to exceed human capabilities. Absolute classification from the entire set of 117 textures generally required a small number of well-chosen exploratory movements (median = 5) and yielded a 95.4% success rate. The method of Bayesian exploration developed and tested in this paper may generalize well to other cognitive problems.

Bayesian Exploration for Intelligent Identification of Textures

PubMed Central

Fishel, Jeremy A.; Loeb, Gerald E.

2012-01-01

In order to endow robots with human-like abilities to characterize and identify objects, they must be provided with tactile sensors and intelligent algorithms to select, control, and interpret data from useful exploratory movements. Humans make informed decisions on the sequence of exploratory movements that would yield the most information for the task, depending on what the object may be and prior knowledge of what to expect from possible exploratory movements. This study is focused on texture discrimination, a subset of a much larger group of exploratory movements and percepts that humans use to discriminate, characterize, and identify objects. Using a testbed equipped with a biologically inspired tactile sensor (the BioTac), we produced sliding movements similar to those that humans make when exploring textures. Measurement of tactile vibrations and reaction forces when exploring textures were used to extract measures of textural properties inspired from psychophysical literature (traction, roughness, and fineness). Different combinations of normal force and velocity were identified to be useful for each of these three properties. A total of 117 textures were explored with these three movements to create a database of prior experience to use for identifying these same textures in future encounters. When exploring a texture, the discrimination algorithm adaptively selects the optimal movement to make and property to measure based on previous experience to differentiate the texture from a set of plausible candidates, a process we call Bayesian exploration. Performance of 99.6% in correctly discriminating pairs of similar textures was found to exceed human capabilities. Absolute classification from the entire set of 117 textures generally required a small number of well-chosen exploratory movements (median = 5) and yielded a 95.4% success rate. The method of Bayesian exploration developed and tested in this paper may generalize well to other cognitive problems. PMID:22783186

The Department of Energy`s Remedial Action Assessment System (RAAS): Decision support tools for performing streamlined feasibility studies

DOE Office of Scientific and Technical Information (OSTI.GOV)

White, M.K.

1994-06-01

The United States Department of Energy (DOE) faces the major task of cleaning up hundreds of waste sites across the nation, which will require completion of a large number of remedial investigation/feasibility studies (RI/FSs). The intent of each RI/FS is to characterize the waste problems and environmental conditions at the operable unit level, segment the remediation problem into manageable medium-specific and contaminant-specific pieces, define corresponding remediation objectives, and identify remedial response actions to satisfy those objectives. The RI/FS team can then identify combinations of remediation technologies that will meet the remediation objectives. Finally, the team must evaluate these remedial alternativesmore » in terms of effectiveness, implementability, cost, and acceptability. The Remedial Action Assessment System (RAAS) is being developed by Pacific Northwest Laboratory (PNL) to support DOE in this effort.« less

Effects of intravenous Escherichia coli dose on the pathophysiological response of colostrum-fed Jersey calves

USDA-ARS?s Scientific Manuscript database

Objectives of the present study were to characterize the dose dependency of an intravenous Escherichia coli (E. coli) challenge in colostrum-fed Jersey calves and to identify biochemical markers indicative of septicemia. Eighteen 3-wk old colostrum-fed Jersey calves were completely randomized to 1 o...

Associate Residency Training Directors in Psychiatry: Demographics, Professional Activities, and Job Satisfaction

ERIC Educational Resources Information Center

Arbuckle, Melissa R.; DeGolia, Sallie G.; Esposito, Karin; Miller, Deborah A.; Weinberg, Michael; Brenner, Adam M.

2012-01-01

Objective: The purpose of this study was to characterize associate training director (ATD) positions in psychiatry. Method: An on-line survey was e-mailed in 2009 to all ATDs identified through the American Association of Directors of Psychiatric Residency Training (AADPRT). Survey questions elicited information regarding demographics,…

Prevalence of nontyphoidal Salmonella and Salmonella strains with conjugative antimicrobial-resistant serovars contaminating animal feed in Texas

USDA-ARS?s Scientific Manuscript database

The objective of this study was to characterize 365 nontyphoidal Salmonella enterica isolates from animal feed. Among the 365 isolates, 78 serovars were identified. Twenty-four isolates (7.0%) were recovered from three of six medicated feed types. Three of these isolates derived from the medicate...

Characterization of Pythium spp. collected from corn and soybean soil in Illinois

USDA-ARS?s Scientific Manuscript database

Pythium root rot is widely distributed in major soybean (Glycine max) production areas throughout the world. There are many species of Pythium described on soybean and other crops, although not all species are pathogenic on all crops. The objectives of this study were to isolate, identify, and evalu...

A Market Strategy Plan For Wayne County Community College. Volume 1.

ERIC Educational Resources Information Center

Nussbaum, Harvey; And Others

In response to a changing environment characterized by economic uncertainty, a declining population, heavy competition, and rising costs, Wayne County Community College (WCCC) developed a market strategy plan to more effectively position and publicize the college. The three main objectives of the plan were to identify WCCC's market segment,…

Phenotypic Differences in Individuals with Autism Spectrum Disorder Born Preterm and at Term Gestation

ERIC Educational Resources Information Center

Bowers, Katherine; Wink, Logan K.; Pottenger, Amy; McDougle, Christopher J.; Erickson, Craig

2015-01-01

The objective of the study was to characterize the phenotype of males and females with autism spectrum disorder born preterm versus those born at term. Descriptive statistical analyses identified differences between male and female autism spectrum disorder subjects born preterm compared to term for several phenotypic characteristics and…

A SYSTEMS APPROACH TO CHARACTERIZING AND PREDICTING THYROID TOXICITY USING AN AMPHIBIAN MODEL

EPA Science Inventory

The EPA was recently mandated to evaluate the potential effects of chemicals on endocrine function and has identified Xenopus as a model organism to use as the basis for a thyroid disruption screening assay. The main objective of this work is to develop a hypothalamic-pituitary-t...

Landscape-scale carbon sampling strategy-lessons learned. Chapter 17

Treesearch

John B. Bradford; Peter Weishampel; Marie-Louise Smith; Randall Kolka; David Y. Hollinger; Richard A. Birdsey; Scott Ollinger; Michael Ryan

2008-01-01

Previous chapters examined individual processes relevant to forest carbon cycling, and characterized measurement approaches for understanding those processes at landscape scales. In this final chapter, we address our overall approach to understanding forest carbon dynamics over large areas. Our objective is to identify any lessons that we learned in the course of...

The Key Roles in the Informal Organization: A Network Analysis Perspective

ERIC Educational Resources Information Center

de Toni, Alberto F.; Nonino, Fabio

2010-01-01

Purpose: The purpose of this paper is to identify the key roles embedded in the informal organizational structure (informal networks) and to outline their contribution in the companies' performance. A major objective of the research is to find and characterize a new key informal role that synthesises problem solving, expertise, and accessibility…

Association of serum vitamin D with the risk of incident dementia and subclinical indices of brain aging the framingham heart study

USDA-ARS?s Scientific Manuscript database

Background: Identifying nutrition- and lifestyle-based risk factors for cognitive impairment and dementia may aid future primary prevention efforts. Objective: We aimed to examine the association of serum vitamin D levels with incident all-cause dementia, clinically characterized Alzheimer's disease...

A Neo-Piagetian Approach to Development During the Formal Operational Period.

ERIC Educational Resources Information Center

Biemiller, Andrew

Cognitive development during each of the major stages identified by Piaget is characterized by abilities to solve progressively more complex tasks (e.g., changes in the object concept during the sensori-motor stage, and in conservation of amount, weight, and volume during the concrete operational stage). Several theorists have suggested that these…

Proteomic characterization of hempseed (Cannabis sativa L.).

PubMed

Aiello, Gilda; Fasoli, Elisa; Boschin, Giovanna; Lammi, Carmen; Zanoni, Chiara; Citterio, Attilio; Arnoldi, Anna

2016-09-16

This paper presents an investigation on hempseed proteome. The experimental approach, based on combinatorial peptide ligand libraries (CPLLs), SDS-PAGE separation, nLC-ESI-MS/MS identification, and database search, permitted identifying in total 181 expressed proteins. This very large number of identifications was achieved by searching in two databases: Cannabis sativa L. (56 gene products identified) and Arabidopsis thaliana (125 gene products identified). By performing a protein-protein association network analysis using the STRING software, it was possible to build the first interactomic map of all detected proteins, characterized by 137 nodes and 410 interactions. Finally, a Gene Ontology analysis of the identified species permitted to classify their molecular functions: the great majority is involved in the seed metabolic processes (41%), responses to stimulus (8%), and biological process (7%). Hempseed is an underexploited non-legume protein-rich seed. Although its protein is well known for its digestibility, essential amino acid composition, and useful techno-functional properties, a comprehensive proteome characterization is still lacking. The objective of this work was to fill this knowledge gap and provide information useful for a better exploitation of this seed in different food products. Copyright © 2016 Elsevier B.V. All rights reserved.

Variability of Massive Young Stellar Objects in Cygnus-X

NASA Astrophysics Data System (ADS)

Thomas, Nancy H.; Hora, J. L.; Smith, H. A.

2013-01-01

Young stellar objects (YSOs) are stars in the process of formation. Several recent investigations have shown a high rate of photometric variability in YSOs at near- and mid-infrared wavelengths. Theoretical models for the formation of massive stars (1-10 solar masses) remain highly idealized, and little is known about the mechanisms that produce the variability. An ongoing Spitzer Space Telescope program is studying massive star formation in the Cygnus-X region. In conjunction with the Spitzer observations, we have conducted a ground-based near-infrared observing program of the Cygnus-X DR21 field using PAIRITEL, the automated infrared telescope at Whipple Observatory. Using the Stetson index for variability, we identified variable objects and a number of variable YSOs in our time-series PAIRITEL data of DR21. We have searched for periodicity among our variable objects using the Lomb-Scargle algorithm, and identified periodic variable objects with an average period of 8.07 days. Characterization of these variable and periodic objects will help constrain models of star formation present. This work is supported in part by the NSF REU and DOD ASSURE programs under NSF grant no. 0754568 and by the Smithsonian Institution.

Identification and Characterization of Unique Subgroups of Chronic Pain Individuals with Dispositional Personality Traits.

PubMed

Mehta, S; Rice, D; McIntyre, A; Getty, H; Speechley, M; Sequeira, K; Shapiro, A P; Morley-Forster, P; Teasell, R W

2016-01-01

Objective. The current study attempted to identify and characterize distinct CP subgroups based on their level of dispositional personality traits. The secondary objective was to compare the difference among the subgroups in mood, coping, and disability. Methods. Individuals with chronic pain were assessed for demographic, psychosocial, and personality measures. A two-step cluster analysis was conducted in order to identify distinct subgroups of patients based on their level of personality traits. Differences in clinical outcomes were compared using the multivariate analysis of variance based on cluster membership. Results. In 229 participants, three clusters were formed. No significant difference was seen among the clusters on patient demographic factors including age, sex, relationship status, duration of pain, and pain intensity. Those with high levels of dispositional personality traits had greater levels of mood impairment compared to the other two groups (p < 0.05). Significant difference in disability was seen between the subgroups. Conclusions. The study identified a high risk group of CP individuals whose level of personality traits significantly correlated with impaired mood and coping. Use of pharmacological treatment alone may not be successful in improving clinical outcomes among these individuals. Instead, a more comprehensive treatment involving psychological treatments may be important in managing the personality traits that interfere with recovery.

Identification of metallic items that caused nickel dermatitis in Danish patients.

PubMed

Thyssen, Jacob P; Menné, Torkil; Johansen, Jeanne D

2010-09-01

Nickel allergy is prevalent as assessed by epidemiological studies. In an attempt to further identify and characterize sources that may result in nickel allergy and dermatitis, we analysed items identified by nickel-allergic dermatitis patients as causative of nickel dermatitis by using the dimethylglyoxime (DMG) test. Dermatitis patients with nickel allergy of current relevance were identified over a 2-year period in a tertiary referral patch test centre. When possible, their work tools and personal items were examined with the DMG test. Among 95 nickel-allergic dermatitis patients, 70 (73.7%) had metallic items investigated for nickel release. A total of 151 items were investigated, and 66 (43.7%) gave positive DMG test reactions. Objects were nearly all purchased or acquired after the introduction of the EU Nickel Directive. Only one object had been inherited, and only two objects had been purchased outside of Denmark. DMG testing is valuable as a screening test for nickel release and should be used to identify relevant exposures in nickel-allergic patients. Mainly consumer items, but also work tools used in an occupational setting, released nickel in dermatitis patients. This study confirmed 'risk items' from previous studies, including mobile phones.

Log analysis of six boreholes in conjunction with geologic characterization above and on top of the Weeks Island Salt Dome

DOE Office of Scientific and Technical Information (OSTI.GOV)

Sattler, A.R.

1996-06-01

Six boreholes were drilled during the geologic characterization and diagnostics of the Weeks Island sinkhole that is over the two-tiered salt mine which was converted for oil storage by the U.S. Strategic Petroleum Reserve. These holes were drilled to provide for geologic characterization of the Weeks Island Salt Dome and its overburden in the immediate vicinity of the sinkhole (mainly through logs and core); to establish a crosswell configuration for seismic tomography; to establish locations for hydrocarbon detection and tracer injection; and to provide direct observations of sinkhole geometry and material properties. Specific objectives of the logging program were to:more » (1) identify the top of and the physical state of the salt dome; (2) identify the water table; (3) obtain a relative salinity profile in the aquifer within the alluvium, which ranges from the water table directly to the top of the Weeks Island salt dome; and (4) identify a reflecting horizon seen on seismic profiles over this salt dome. Natural gamma, neutron, density, sonic, resistivity and caliper logs were run.« less

Sleep, Dietary, and Exercise Behavioral Clusters among Truck Drivers with Obesity: Implications for Interventions

PubMed Central

Olson, Ryan; Thompson, Sharon V.; Wipfli, Brad; Hanson, Ginger; Elliot, Diane L.; Anger, W. Kent; Bodner, Todd; Hammer, Leslie B.; Hohn, Elliot; Perrin, Nancy A.

2015-01-01

Objective Our objectives were to describe a sample of truck drivers, identify clusters of drivers with similar patterns in behaviors affecting energy balance (sleep, diet, and exercise), and test for cluster differences in health and psychosocial factors. Methods Participants’ (n=452, BMI M=37.2, 86.4% male) self-reported behaviors were dichotomized prior to hierarchical cluster analysis, which identified groups with similar behavior co-variation. Cluster differences were tested with generalized estimating equations. Results Five behavioral clusters were identified that differed significantly in age, smoking status, diabetes prevalence, lost work days, stress, and social support, but not in BMI. Cluster 2, characterized by the best sleep quality, had significantly lower lost workdays and stress than other clusters. Conclusions Weight management interventions for drivers should explicitly address sleep, and may be maximally effective after establishing socially supportive work environments that reduce stress exposures. PMID:26949883

Learning from examples - Generation and evaluation of decision trees for software resource analysis

NASA Technical Reports Server (NTRS)

Selby, Richard W.; Porter, Adam A.

1988-01-01

A general solution method for the automatic generation of decision (or classification) trees is investigated. The approach is to provide insights through in-depth empirical characterization and evaluation of decision trees for software resource data analysis. The trees identify classes of objects (software modules) that had high development effort. Sixteen software systems ranging from 3,000 to 112,000 source lines were selected for analysis from a NASA production environment. The collection and analysis of 74 attributes (or metrics), for over 4,700 objects, captured information about the development effort, faults, changes, design style, and implementation style. A total of 9,600 decision trees were automatically generated and evaluated. The trees correctly identified 79.3 percent of the software modules that had high development effort or faults, and the trees generated from the best parameter combinations correctly identified 88.4 percent of the modules on the average.

High resolution Talbot self-imaging applied to structural characterization of self-assembled monolayers of microspheres.

PubMed

Garcia-Sucerquia, J; Alvarez-Palacio, D C; Kreuzer, H J

2008-09-10

We report the observation of the Talbot self-imaging effect in high resolution digital in-line holographic microscopy (DIHM) and its application to structural characterization of periodic samples. Holograms of self-assembled monolayers of micron-sized polystyrene spheres are reconstructed at different image planes. The point-source method of DIHM and the consequent high lateral resolution allows the true image (object) plane to be identified. The Talbot effect is then exploited to improve the evaluation of the pitch of the assembly and to examine defects in its periodicity.

Learning to explore the structure of kinematic objects in a virtual environment

PubMed Central

Buckmann, Marcus; Gaschler, Robert; Höfer, Sebastian; Loeben, Dennis; Frensch, Peter A.; Brock, Oliver

2015-01-01

The current study tested the quantity and quality of human exploration learning in a virtual environment. Given the everyday experience of humans with physical object exploration, we document substantial practice gains in the time, force, and number of actions needed to classify the structure of virtual chains, marking the joints as revolute, prismatic, or rigid. In line with current work on skill acquisition, participants could generalize the new and efficient psychomotor patterns of object exploration to novel objects. On the one hand, practice gains in exploration performance could be captured by a negative exponential practice function. On the other hand, they could be linked to strategies and strategy change. After quantifying how much was learned in object exploration and identifying the time course of practice-related gains in exploration efficiency (speed), we identified what was learned. First, we identified strategy components that were associated with efficient (fast) exploration performance: sequential processing, simultaneous use of both hands, low use of pulling rather than pushing, and low use of force. Only the latter was beneficial irrespective of the characteristics of the other strategy components. Second, we therefore characterized efficient exploration behavior by strategies that simultaneously take into account the abovementioned strategy components. We observed that participants maintained a high level of flexibility, sampling from a pool of exploration strategies trading the level of psycho-motoric challenges with exploration speed. We discuss the findings pursuing the aim of advancing intelligent object exploration by combining analytic (object exploration in humans) and synthetic work (object exploration in robots) in the same virtual environment. PMID:25904878

Advanced LIGO low-latency searches

NASA Astrophysics Data System (ADS)

Kanner, Jonah; LIGO Scientific Collaboration, Virgo Collaboration

2016-06-01

Advanced LIGO recently made the first detection of gravitational waves from merging binary black holes. The signal was first identified by a low-latency analysis, which identifies gravitational-wave transients within a few minutes of data collection. More generally, Advanced LIGO transients are sought with a suite of automated tools, which collectively identify events, evaluate statistical significance, estimate source position, and attempt to characterize source properties. This low-latency effort is enabling a broad multi-messenger approach to the science of compact object mergers and other transients. This talk will give an overview of the low-latency methodology and recent results.

Spectral Characteristics of Young Stars Associated with the Sh2-296 Nebula

NASA Astrophysics Data System (ADS)

Fernandes, Beatriz; Gregorio-Hetem, Jane

Aiming to contribute to the understanding of star formation and evolution in the Canis Major (CMa R1) Molecular Clouds Complex, we analyze the spectral characteristics of a population of young stars associated with the arc-shaped nebula Sh2-296. Our XMM/Newton observations detected 109 X-ray sources in the region and optical spectroscopy was performed with Gemini telescope for 85 optical counterparts. We identified and characterized 51 objects that present features typically found in young objects, such as Hα emission and strong absorption on the Li I line.

Trajectories of Symptoms and Impairment for Pediatric Patients with Functional Abdominal Pain: A 5-Year Longitudinal Study

ERIC Educational Resources Information Center

Mulvaney, Shelagh; Lambert, E. Warren; Garber, Judy; Walker, Lynn S.

2006-01-01

Objective: This prospective study characterizes trajectories of symptoms and impairment in pediatric patients with abdominal pain not associated with identifiable organic disease. Method: The Children's Somatization Inventory and the Functional Disability Inventory were administered four times over 5 years to 132 patients (6-18 years old) seen in…

Social Anxiety Disorder and Social Skills: A Critical Review of the Literature

ERIC Educational Resources Information Center

Angelico, Antonio Paulo; Crippa, Jose Alexandre S.; Loureiro, Sonia Regina

2013-01-01

The objective of this article is to present a critical analysis of the research outlines used in empirical studies published between the years 2000 and March of 2007 about social anxiety disorder and its associations with social skills. Seventeen papers were identified and grouped into two classes for analysis, namely: Characterization of Social…

Development of an Evaluation Tool for Online Food Safety Training Programs

ERIC Educational Resources Information Center

Neal, Jack A., Jr.; Murphy, Cheryl A.; Crandall, Philip G.; O'Bryan, Corliss A.; Keifer, Elizabeth; Ricke, Steven C.

2011-01-01

The objective of this study was to provide the person in charge and food safety instructors an assessment tool to help characterize, identify strengths and weaknesses, determine the completeness of the knowledge gained by the employee, and evaluate the level of content presentation and usability of current retail food safety training platforms. An…

Characterization of Pm59, a novel powdery mildew resistance gene in Afghanistan wheat landrace PI 181356

USDA-ARS?s Scientific Manuscript database

Powdery mildew, caused by Blumeria graminis f. sp. tritici (Bgt), is an important foliar disease of wheat worldwide. In the Great Plains of the USA, Bgt isolates virulent to widely used powdery mildew resistance genes, such as Pm3a, were previously identified. The objectives of this study were to ch...

Computer Vision and Machine Learning for Autonomous Characterization of AM Powder Feedstocks

NASA Astrophysics Data System (ADS)

DeCost, Brian L.; Jain, Harshvardhan; Rollett, Anthony D.; Holm, Elizabeth A.

2017-03-01

By applying computer vision and machine learning methods, we develop a system to characterize powder feedstock materials for metal additive manufacturing (AM). Feature detection and description algorithms are applied to create a microstructural scale image representation that can be used to cluster, compare, and analyze powder micrographs. When applied to eight commercial feedstock powders, the system classifies powder images into the correct material systems with greater than 95% accuracy. The system also identifies both representative and atypical powder images. These results suggest the possibility of measuring variations in powders as a function of processing history, relating microstructural features of powders to properties relevant to their performance in AM processes, and defining objective material standards based on visual images. A significant advantage of the computer vision approach is that it is autonomous, objective, and repeatable.

A superluminous object in the Large Cloud of Magellan

NASA Astrophysics Data System (ADS)

Mathis, J. S.; Savage, B. D.; Cassinelli, J. P.

1984-08-01

The superluminous object R136a of the nebula 30 Dor in the Large Cloud of Magellan is characterized, summarizing the results of recent optical and (IUE) UV observations. Photographs, spectra, and diagrams are provided; and the techniques used to determine the parameters of the object are explained. The UV spectra exhibit a typical P Cygni profile like that of O-type stars, but R136a is much brighter (5 x 10 to the 7th solar luminosity). Speckle interferometry has identified a main component and two fainter objects at distances of 0.5 and 0.1 arcsec. The main component R136a1 is probably either a very massive single star (400-1000 solar mass) or a tight cluster of stars of known types. Evidence for the existence of other similar objects is reviewed.

Resonant ultrasound spectroscopy

DOEpatents

Migliori, Albert

1991-01-01

A resonant ultrasound spectroscopy method provides a unique characterization of an object for use in distinguishing similar objects having physical differences greater than a predetermined tolerance. A resonant response spectrum is obtained for a reference object by placing excitation and detection transducers at any accessible location on the object. The spectrum is analyzed to determine the number of resonant response peaks in a predetermined frequency interval. The distribution of the resonance frequencies is then characterized in a manner effective to form a unique signature of the object. In one characterization, a small frequency interval is defined and stepped though the spectrum frequency range. Subsequent objects are similarly characterized where the characterizations serve as signatures effective to distinguish objects that differ from the reference object by more than the predetermined tolerance.

Tire Crumb Research Study Literature Review / Gap ...

EPA Pesticide Factsheets

In order to more fully understand data gaps in human exposure and toxicity to tire crumb materials, ATSDR, CPSC and EPA undertook a collaborative effort in the form of a scientific literature review and subsequent gaps analysis. The first objective of the Literature Review and Gap Analysis (LRGA) collaboration was to identify the existing body of literature related specifically to human exposure to tire crumb materials through the use of synthetic turf athletic fields and playgrounds. The second objective was to characterize and summarize the relevant data from the scientific literature. The final objective was to review the summary information and identify data gaps to build on the current understanding of the state-of-the-science and inform the development of specific research efforts that would be most impactful in the near-term. Because of the need for additional information, the U.S. Environmental Protection Agency (EPA), the Centers for Disease Control and Prevention/Agency for Toxic Substances and Disease Registry (ATSDR), and the U.S. Consumer Product Safety Commission (CPSC) launched a multi-agency action plan to study key environmental human health questions. The Federal Research Action Plan includes numerous activities, including research studies (U.S. EPA, 2016). A key objective of the Action Plan is to identify key knowledge gaps.

Accumulating Data to Optimally Predict Obesity Treatment (ADOPT) Core Measures: Environmental Domain.

PubMed

Saelens, Brian E; Arteaga, S Sonia; Berrigan, David; Ballard, Rachel M; Gorin, Amy A; Powell-Wiley, Tiffany M; Pratt, Charlotte; Reedy, Jill; Zenk, Shannon N

2018-04-01

There is growing interest in how environment is related to adults' weight and activity and eating behaviors. However, little is known about whether environmental factors are related to the individual variability seen in adults' intentional weight loss or maintenance outcomes. The environmental domain subgroup of the Accumulating Data to Optimally Predict obesity Treatment (ADOPT) Core Measures Project sought to identify a parsimonious set of objective and perceived neighborhood and social environment constructs and corresponding measures to include in the assessment of response to adult weight-loss treatment. Starting with the home address, the environmental domain subgroup recommended for inclusion in future weight-loss or maintenance studies constructs and measures related to walkability, perceived land use mix, food outlet accessibility (perceived and objective), perceived food availability, socioeconomics, and crime-related safety (perceived and objective) to characterize the home neighborhood environment. The subgroup also recommended constructs and measures related to social norms (perceived and objective) and perceived support to characterize an individual's social environment. The 12 neighborhood and social environment constructs and corresponding measures provide a succinct and comprehensive set to allow for more systematic examination of the impact of environment on adults' weight loss and maintenance. © 2018 The Obesity Society.

SUPERFUND INNOVATIVE TECHNOLOGIES EVALUATION ...

EPA Pesticide Factsheets

This task seeks to identify high priority needs of the Regions and Program Offices for innovative field sampling, characterization, monitoring, and measurement technologies. When an appropriate solution to a specific problem is identified, a field demonstration is conducted to document the performance and cost of the proposed technologies. The use of field analysis almost always provides a savings in time and cost over the usual sample and ship to a conventional laboratory for analysis approach to site characterization and monitoring. With improvements in technology and appropriate quality assurance/quality control, field analysis has been shown to provide high quality data, useful for most environmental monitoring or characterization projects. An emphasis of the program is to seek out innovative solutions to existing problems and to provide the cost and performance data a user would require to make an informed decision regarding the adequacy of a technology to address a specific environmental problem. The objective of this program is to promote the acceptance and use of innovative field technologies by providing well-documented performance and cost data obtained from field demonstrations.

Morphological and molecular characterization of Cladosporium cladosporioides species complex causing pecan tree leaf spot.

PubMed

Walker, C; Muniz, M F B; Rolim, J M; Martins, R R O; Rosenthal, V C; Maciel, C G; Mezzomo, R; Reiniger, L R S

2016-09-16

The objective of this study was to characterize species of the Cladosporium cladosporioides complex isolated from pecan trees (Carya illinoinensis) with symptoms of leaf spot, based on morphological and molecular approaches. Morphological attributes were assessed using monosporic cultures on potato dextrose agar medium, which were examined for mycelial growth, sporulation, color, and conidia and ramoconidia size. Molecular characterization comprised isolation of DNA and subsequent amplification of the translation elongation factor 1α (TEF-1α) region. Three species of the C. cladosporioides complex were identified: C. cladosporioides, Cladosporium pseudocladosporioides, and Cladosporium subuliforme. Sporulation was the most important characteristic differentiating species of this genus. However, morphological features must be considered together with molecular analysis, as certain characters are indistinguishable between species. TEF-1αcan be effectively used to identify and group isolates belonging to the C. cladosporioides complex. The present study provides an important example of a methodology to ascertain similarity between isolates of this complex causing leaf spot in pecan trees, which should facilitate future pathogenicity studies.

Characterization and comparison of proteins in the sperm storage tubules of female chickens to bovine epididymal fluid

USDA-ARS?s Scientific Manuscript database

Female birds are able to store sperm in crypts called sperm storage tubules (SSTs) in their reproductive tracts for between two and six weeks. Comparatively, sperm in a cow’s reproductive tract remain viable for between 18 and 24 hours. The objective of this experiment was to try to identify and co...

Participation and Enjoyment of Leisure Activities in School-Aged Children with Cerebral Palsy

ERIC Educational Resources Information Center

Majnemer, Annette; Shevell, Michael; Law, Mary; Birnbaum, Rena; Chilingaryan, Gevorg; Rosenbaum, Peter; Poulin, Chantal

2008-01-01

The objective of this study was to characterize participation in leisure activities in children with cerebral palsy (CP) and identify determinants of greater involvement. Ninety-five children of school age (9y 7mo [SD 2y 1mo]) with CP were recruited, and participation was evaluated with the Children's Assessment of Participation and Enjoyment in a…

Analysis of NHANES Measured Blood PCBs in the General U.S. Population andApplication of SHEDS Model to Identify Key Exposure Factors

EPA Science Inventory

Studies have shown that the U.S. population continues to be exposed to polychlorinated biphenyls (PCBs), despite their ban more than three decades ago, but the reasons are not fully understood. The objectives of this paper are to characterize patterns of PCBs in blood by age, gen...

The Regulation of Gene Expression in Cnidarian-Algal Associations.

DTIC Science & Technology

1998-07-13

symbiotic cnidarians , Aiptasia pallida, Anthopleura eligantissima, synbiosis-specific proteins, cDNA libraries, O. SECURITY CLASSIFICATION OP REPORT...gene expression in cnidarian -algal associations Award Period: 1 July 1995-30 June 1998 Objectives: A. To identify and characterize heat shock...Exploring Symbiosis-Specific Gene Expression in Cnidarian /Algal Associations. In: Molecular Approaches to the Study of the Ocean.. Ed. K. Cooksey, Chapman

Tank 241-AZ-102 Privatization Push Mode Core Sampling and Analysis Plan

DOE Office of Scientific and Technical Information (OSTI.GOV)

RASMUSSEN, J.H.

1999-08-02

This sampling and analysis plan (SAP) identifies characterization objectives pertaining to sample collection, laboratory analytical evaluation, and reporting requirements for samples obtained from tank 241-AZ-102. The purpose of this sampling event is to obtain information about the characteristics of the contents of 241-AZ-102 required to satisfy the Data Quality Objectives For TWRS Privatization Phase I: Confirm Tank TIS An Appropriate Feed Source For High-Level Waste Feed Batch X(HLW DQO) (Nguyen 1999a), Data Quality Objectives For TWRS Privatization Phase 1: Confirm Tank TIS An Appropriate Feed Source For Low-Activity Waste Feed Batch X (LAW DQO) (Nguyen 1999b), Low Activity Waste andmore » High Level Waste Feed Data Quality Objectives (L&H DQO) (Patello et al. 1999) and Characterization Data Needs for Development, Design, and Operation of Retrieval Equipment Developed through the Data Quality Objective Process (Equipment DQO) (Bloom 1996). The Tank Characterization Technical Sampling Basis document (Brown et al. 1998) indicates that these issues, except the Equipment DQO apply to tank 241-AZ-102 for this sampling event. The Equipment DQO is applied for shear strength measurements of the solids segments only. Poppiti (1999) requires additional americium-241 analyses of the sludge segments. Brown et al. (1998) also identify safety screening, regulatory issues and provision of samples to the Privatization Contractor(s) as applicable issues for this tank. However, these issues will not be addressed via this sampling event. Reynolds et al. (1999) concluded that information from previous sampling events was sufficient to satisfy the safety screening requirements for tank 241 -AZ-102. Push mode core samples will be obtained from risers 15C and 24A to provide sufficient material for the chemical analyses and tests required to satisfy these data quality objectives. The 222-S Laboratory will extrude core samples, composite the liquids and solids, perform chemical analyses, and provide subsamples to the Process Chemistry Laboratory. The Process Chemistry Laboratory will prepare test plans and perform process tests to evaluate the behavior of the 241-AZ-102 waste undergoing the retrieval and treatment scenarios defined in the applicable DQOs. Requirements for analyses of samples originating in the process tests will be documented in the corresponding test plan.« less

Characterizing Students' Attempts to Explain Observations from Practical Work: Intermediate Phases of Understanding

NASA Astrophysics Data System (ADS)

Mestad, Idar; Kolstø, Stein Dankert

2017-10-01

This study aims to characterize a group of students' preliminary oral explanations of a scientific phenomenon produced as part of their learning process. The students were encouraged to use their own wordings to test out their own interpretation of observations when conducting practical activities. They presented their explanations orally in the whole class after having discussed and written down an explanation in a small group. The data consists of transcribed video recordings of the presented explanations, observation notes, and interviews. A genre perspective was used to characterize the students' explanations together with analysis of the students use of scientific terms, gestures, and the language markers "sort of" and "like." Based on the analysis we argue to separate between event-focused explanations, where the students describe how objects move, and object-focused explanations, where the students describe object properties and interactions. The first type uses observable events and few scientific terms, while the latter contains object properties and tentative use of scientific terms. Both types are accompanied by an extensive use of language markers and gestures. A third category, term-focused explanations, is used when the students only provide superficial explanations by expressing scientific terms. Here, the students' use of language markers and gestures are low. The analyses shows how students' explanations can be understood as tentative attempts to build on their current understanding and observations while trying to reach out for a deeper and scientific way of identifying observations and building explanations and new ways of talking.

Patterns of muscle activity underlying object-specific grasp by the macaque monkey.

PubMed

Brochier, T; Spinks, R L; Umilta, M A; Lemon, R N

2004-09-01

During object grasp, a coordinated activation of distal muscles is required to shape the hand in relation to the physical properties of the object. Despite the fundamental importance of the grasping action, little is known of the muscular activation patterns that allow objects of different sizes and shapes to be grasped. In a study of two adult macaque monkeys, we investigated whether we could distinguish between EMG activation patterns associated with grasp of 12 differently shaped objects, chosen to evoke a wide range of grasping postures. Each object was mounted on a horizontal shuttle held by a weak spring (load force 1-2 N). Objects were located in separate sectors of a "carousel," and inter-trial rotation of the carousel allowed sequential presentation of the objects in pseudorandom order. EMG activity from 10 to 12 digit, hand, and arm muscles was recorded using chronically implanted electrodes. We show that the grasp of different objects was characterized by complex but distinctive patterns of EMG activation. Cluster analysis shows that these object-related EMG patterns were specific and consistent enough to identify the object unequivocally from the EMG recordings alone. EMG-based object identification required a minimum of six EMGs from simultaneously recorded muscles. EMG patterns were consistent across recording sessions in a given monkey but showed some differences between animals. These results identify the specific patterns of activity required to achieve distinct hand postures for grasping, and they open the way to our understanding of how these patterns are generated by the central motor network.

Report of sampling and analysis results, Addison Army housing units, Addison, Illinois. Final report

DOE Office of Scientific and Technical Information (OSTI.GOV)

Not Available

1990-09-01

The objectives of this sampling and analysis effort include further characterization of environmental contamination identified in an enhanced preliminary assessment carried out in 1989. The specific activities performed at this site were identification, evaluation of the condition, and collection of samples from specific suspected asbestos-containing materials, including floor tiles, pipe run and pipe fitting insulation, dust in the ductwork, and exterior siding, where present. These evaluation were necessary to clarify potential environmental issues identified in the earlier report, prior to the sale or realignment of the property.

Compositional Investigation of Binary Near-Earth Asteroid 66063 (1998 RO1): A Potentially Undifferentiated Assemblage

NASA Technical Reports Server (NTRS)

Abell, P. A.; Gaffey, M. J.; Landis, R. R.; Jarvis, K. S.

2005-01-01

It is now thought that approximately 16% of all asteroids among the near-Earth population may be binary objects. Several independent lines of evidence, such as the presence of doublet craters on the Earth and Moon [1, 2], complex lightcurves of near-Earth objects exhibiting mutual events [3], and radar images of near-Earth asteroids revealing distinct primary and secondary objects, have supported this conclusion [4]. To date at least 23 near-Earth objects have been discovered as binary systems with expectations that many more have yet to be identified or recognized. Little is known about the physical characteristics of binary objects except that they seem to have fairly rapid rotation rates, generally have primaries in the approx. 1 km diameter range with smaller secondaries on the order of a few hundred meters, and apart from a few exceptions, are in synchronous orbits [4, 5]. Previously only two of these binary near-Earth asteroids (1998 ST27 and 2003 YT1) have been characterized in terms of detailed mineralogical investigations [6, 7]. Such investigations are required to fully understand the formation mechanisms of these binary objects and their possible source regions. In addition, detailed knowledge of these objects may play an important role for planning future spacecraft missions and for the development of impact mitigation strategies. The work presented here represents a continued effort to characterize this particular sub-group of the near- Earth asteroid population.

Interpretation of Spectrometric Measurements of Active Geostationary Satellites

NASA Astrophysics Data System (ADS)

Bedard, D.; Wade, G.

2014-09-01

Over 5000 visible near-infrared (VNIR) spectrometric measurements of active geostationary satellites have been collected with the National Research Council (NRC) 1.8m Plaskett telescope located at the Dominion Astrophysical Observatory (DAO) in Victoria, Canada. The objective of this ongoing experiment is to study how reflectance spectroscopy can be used to reliably identify specific material types on the surface of artificial Earth-orbiting objects. Active geostationary satellites were selected as the main subjects for this experiment since their orientation is stable and can be estimated to a high-level of confidence throughout a night of observation. Furthermore, for most geostationary satellites, there is a wide variety of sources that can provide some level of information as to their external surface composition. Notwithstanding the high number of measurements that have been collected to date, it was assumed that the experimenters would have a much greater success rate in material identification given the choice experimental subjects. To date, only the presence of aluminum has been confidently identified in some of the reflectance spectra that have been collected. Two additional material types, namely photovoltaic cells and polyimide film, the first layer of multi-layer insulation (MLI), have also been possibly identified. However uncertainties in the reduced spectral measurements prevent any definitive conclusion with respect to these materials at this time. The surprising lack of results with respect to material identification have forced the experimenters to use other data interpretation methods to characterize the spectral scattering characteristics of the studied satellites. The results from this study have already led to improvements in the ways that reflectance spectra from spacecraft are collected and analysed. Equally important, the data interpretation techniques elaborated over the course of this experiment will also serve to increase the body of knowledge pertaining to the spectral characterization of artificial Earth-orbiting objects.

Relevant Scatterers Characterization in SAR Images

NASA Astrophysics Data System (ADS)

Chaabouni, Houda; Datcu, Mihai

2006-11-01

Recognizing scenes in a single look meter resolution Synthetic Aperture Radar (SAR) images, requires the capability to identify relevant signal signatures in condition of variable image acquisition geometry, arbitrary objects poses and configurations. Among the methods to detect relevant scatterers in SAR images, we can mention the internal coherence. The SAR spectrum splitted in azimuth generates a series of images which preserve high coherence only for particular object scattering. The detection of relevant scatterers can be done by correlation study or Independent Component Analysis (ICA) methods. The present article deals with the state of the art for SAR internal correlation analysis and proposes further extensions using elements of inference based on information theory applied to complex valued signals. The set of azimuth looks images is analyzed using mutual information measures and an equivalent channel capacity is derived. The localization of the "target" requires analysis in a small image window, thus resulting in imprecise estimation of the second order statistics of the signal. For a better precision, a Hausdorff measure is introduced. The method is applied to detect and characterize relevant objects in urban areas.

A Framework for Integrating Environmental Justice in Regulatory Analysis

PubMed Central

Nweke, Onyemaechi C.

2011-01-01

With increased interest in integrating environmental justice into the process for developing environmental regulations in the United States, analysts and decision makers are confronted with the question of what methods and data can be used to assess disproportionate environmental health impacts. However, as a first step to identifying data and methods, it is important that analysts understand what information on equity impacts is needed for decision making. Such knowledge originates from clearly stated equity objectives and the reflection of those objectives throughout the analytical activities that characterize Regulatory Impact Analysis (RIA), a process that is traditionally used to inform decision making. The framework proposed in this paper advocates structuring analyses to explicitly provide pre-defined output on equity impacts. Specifically, the proposed framework emphasizes: (a) defining equity objectives for the proposed regulatory action at the onset of the regulatory process, (b) identifying specific and related sub-objectives for key analytical steps in the RIA process, and (c) developing explicit analytical/research questions to assure that stated sub-objectives and objectives are met. In proposing this framework, it is envisioned that information on equity impacts informs decision-making in regulatory development, and that this is achieved through a systematic and consistent approach that assures linkages between stated equity objectives, regulatory analyses, selection of policy options, and the design of compliance and enforcement activities. PMID:21776235

Molecular and Phenotypic Characterization of Phialemonium and Lecythophora Isolates from Clinical Samples▿

PubMed Central

Perdomo, H.; Sutton, D. A.; García, D.; Fothergill, A. W.; Gené, J.; Cano, J.; Summerbell, R. C.; Rinaldi, M. G.; Guarro, J.

2011-01-01

Several members of the fungal genera Phialemonium and Lecythophora are occasional agents of severe human and animal infections. These species are difficult to identify, and relatively little is known about their frequency in the clinical setting. The objective of this study was to characterize morphologically and molecularly, on the basis of the analysis of large-subunit ribosomal DNA sequences, a set of 68 clinical isolates presumed to belong to these genera. A total of 59 isolates were determined to be Phialemonium species (n = 32) or a related Cephalotheca species (n = 6) or Lecythophora species (n = 20) or a related Coniochaeta species (n = 1). Nine isolates identified to be Acremonium spp. or Phaeoacremonium spp. were excluded from further study. The most common species were Phialemonium obovatum and Phialemonium curvatum, followed by Lecythophora hoffmannii, Cephalotheca foveolata, and Lecythophora mutabilis. PMID:21270235

Tactile Imaging Markers to Characterize Female Pelvic Floor Conditions.

PubMed

van Raalte, Heather; Egorov, Vladimir

2015-08-01

The Vaginal Tactile Imager (VTI) records pressure patterns from vaginal walls under an applied tissue deformation and during pelvic floor muscle contractions. The objective of this study is to validate tactile imaging and muscle contraction parameters (markers) sensitive to the female pelvic floor conditions. Twenty-two women with normal and prolapse conditions were examined by a vaginal tactile imaging probe. We identified 9 parameters which were sensitive to prolapse conditions ( p < 0.05 for one-way ANOVA and/or p < 0.05 for t -test with correlation factor r from -0.73 to -0.56). The list of parameters includes pressure, pressure gradient and dynamic pressure response during muscle contraction at identified locations. These parameters may be used for biomechanical characterization of female pelvic floor conditions to support an effective management of pelvic floor prolapse.

Tactile Imaging Markers to Characterize Female Pelvic Floor Conditions

PubMed Central

van Raalte, Heather; Egorov, Vladimir

2015-01-01

The Vaginal Tactile Imager (VTI) records pressure patterns from vaginal walls under an applied tissue deformation and during pelvic floor muscle contractions. The objective of this study is to validate tactile imaging and muscle contraction parameters (markers) sensitive to the female pelvic floor conditions. Twenty-two women with normal and prolapse conditions were examined by a vaginal tactile imaging probe. We identified 9 parameters which were sensitive to prolapse conditions (p < 0.05 for one-way ANOVA and/or p < 0.05 for t-test with correlation factor r from −0.73 to −0.56). The list of parameters includes pressure, pressure gradient and dynamic pressure response during muscle contraction at identified locations. These parameters may be used for biomechanical characterization of female pelvic floor conditions to support an effective management of pelvic floor prolapse. PMID:26389014

Anti-inflammatory and antioxidant properties of Piper species: a perspective from screening to molecular mechanisms.

PubMed

Kumar, Sarvesh; Malhotra, Shashwat; Prasad, Ashok K; Van der Eycken, Erik V; Bracke, Marc E; Stetler-Stevenson, William G; Parmar, Virinder S; Ghosh, Balaram

2015-01-01

Identifying novel therapeutic agents from natural sources and their possible intervention studies has been one of the major areas in biomedical research in recent years. Piper species are highly important - commercially, economically and medicinally. Our groups have been working for more than two decades on the identification and characterization of novel therapeutic lead molecules from Piper species. We have extensively studied the biological activities of various extracts of Piper longum and Piper galeatum, and identified and characterized novel molecules from these species. Using synthetic chemistry, various functional groups of the lead molecules were modified and structure activity relationship (SAR) studies identified synthetic molecules with better efficacy and lower IC50 values. Moreover, the mechanisms of actions of some of these molecules were studied at the molecular level. The objective of this review is to summarize experimental data published from our laboratories and others on antioxidant and anti-inflammatory potentials of Piper species and their chemical constituents.

Behavioral and biological interactions with small groups in confined microsocieties

NASA Technical Reports Server (NTRS)

Brady, J. V.; Emurian, H. H.

1982-01-01

Requirements for high levels of human performance in the unfamiliar and stressful environments associated with space missions necessitate the development of research-based technological procedures for maximizing the probability of effective functioning at all levels of personnel participation. Where the successful accomplishment of such missions requires the coordinated contributions of several individuals collectively identified with the achievement of a common objective, the conditions for characterizing a team, crew, or functional group are operationally defined. For the most part, studies of group performances under operational conditions which emphasize relatively long exposure to extended mission environments have been limited by the constraints imposed on experimental manipulations to identify critical effectiveness factors. On the other hand, laboratory studies involving relatively brief exposures to contrived task situations have been considered of questionable generality to operational settings requiring realistic group objectives.

The Coldest Discoveries of Backyard Worlds

NASA Astrophysics Data System (ADS)

Faherty, Jacqueline; Kuchner, Marc; Logsdon, Sarah; Bardalez Gagliuffi, Daniella; Meisner, Aaron; Schneider, Adam; Gagne, Jonathan; Caselden, Dan

2018-05-01

Over the past five years, our view of the local solar neighborhood has changed drastically thanks to the Wide Field Infrared Survey Explorer (WISE). Not only did WISE redefine the five closest systems to the Sun with the discovery of Luhman16AB and WISE0855, but it also revealed the Sun's closest fly by and it defined an entirely new class of extremely cold and close compact objects: the Y dwarfs. At present, all but two of the Y dwarfs were discovered through a WISE single epoch photometric identification. As a whole, these objects are of critical importance to identifying the efficiency of star formation at the lowest masses. As individuals, each of these objects represents a unique probe into the complex chemistry present in the coldest photospheres produced in the Universe. Every single Y dwarf discovered is an observational treasure. In February 2017, we launched a citizen science project called Backyard Worlds: Planet 9 with the intention of scanning the entire sky using not just photometric information but positional as well to identify the coldest, closest, and fastest moving objects near the Sun. In this Spitzer Cycle 14 proposal, we have identified 65 tantalizingly fast moving objects that appear to be extremely cold hence potentially among the Sun's nearest neighbors. We ask for 26.8 hours with Spitzer to obtain critical ch1 and ch2 photometry as well as astrometric information (to confirm motion) which will allow us to characterize these new cold brown dwarf neighbors. The coldest objects discovered through this proposal will be important follow-up targets for JWST spectroscopy.

Kepler AutoRegressive Planet Search

NASA Astrophysics Data System (ADS)

Feigelson, Eric

NASA's Kepler mission is the source of more exoplanets than any other instrument, but the discovery depends on complex statistical analysis procedures embedded in the Kepler pipeline. A particular challenge is mitigating irregular stellar variability without loss of sensitivity to faint periodic planetary transits. This proposal presents a two-stage alternative analysis procedure. First, parametric autoregressive ARFIMA models, commonly used in econometrics, remove most of the stellar variations. Second, a novel matched filter is used to create a periodogram from which transit-like periodicities are identified. This analysis procedure, the Kepler AutoRegressive Planet Search (KARPS), is confirming most of the Kepler Objects of Interest and is expected to identify additional planetary candidates. The proposed research will complete application of the KARPS methodology to the prime Kepler mission light curves of 200,000: stars, and compare the results with Kepler Objects of Interest obtained with the Kepler pipeline. We will then conduct a variety of astronomical studies based on the KARPS results. Important subsamples will be extracted including Habitable Zone planets, hot super-Earths, grazing-transit hot Jupiters, and multi-planet systems. Groundbased spectroscopy of poorly studied candidates will be performed to better characterize the host stars. Studies of stellar variability will then be pursued based on KARPS analysis. The autocorrelation function and nonstationarity measures will be used to identify spotted stars at different stages of autoregressive modeling. Periodic variables with folded light curves inconsistent with planetary transits will be identified; they may be eclipsing or mutually-illuminating binary star systems. Classification of stellar variables with KARPS-derived statistical properties will be attempted. KARPS procedures will then be applied to archived K2 data to identify planetary transits and characterize stellar variability.

Molecular genotyping of ABO blood groups in some population groups from India

PubMed Central

Ray, Sabita; Gorakshakar, Ajit C.; Vasantha, K.; Nadkarni, Anita; Italia, Yazdi; Ghosh, Kanjaksha

2014-01-01

Background & objectives: Indian population is characterized by the presence of various castes and tribal groups. Various genetic polymorphisms have been used to differentiate among these groups. Amongst these, the ABO blood group system has been extensively studied. There is no information on molecular genotyping of ABO blood groups from India. Therefore, the main objective of this study was to characterize the common A, B and O alleles by molecular analysis in some Indian population groups. Methods: One hundred samples from the mixed population from Mumbai, 101 samples from the Dhodia tribe and 100 samples from the Parsi community were included in this study. Initially, the samples were phenotyped by standard serologic techniques. PCR followed by single strand conformational polymorphsim (SSCP) was used for molecular ABO genotyping. Samples showing atypical SSCP patterns were further analysed by DNA sequencing to characterize rare alleles. Results: Seven common ABO alleles with 19 different genotypes were found in the mixed population. The Dhodias showed 12 different ABO genotypes and the Parsis revealed 15 different ABO genotypes with six common ABO alleles identified in each of them. Two rare alleles were also identified. Interpretation & conclusions: This study reports the distribution of molecular genotypes of ABO alleles among some population groups from India. Considering the extremely heterogeneous nature of the Indian population, in terms of various genotype markers like blood groups, red cell enzymes, etc., many more ABO alleles are likely to be encountered. PMID:24604045

An Objective Approach for Burkholderia pseudomallei Strain Selection as Challenge Material for Medical Countermeasures Efficacy Testing

PubMed Central

Van Zandt, Kristopher E.; Tuanyok, Apichai; Keim, Paul S.; Warren, Richard L.; Gelhaus, H. Carl

2012-01-01

Burkholderia pseudomallei is the causative agent of melioidosis, a rare disease of biodefense concern with high mortality and extreme difficulty in treatment. No human vaccines are available that protect against B. pseudomallei infection, and with the current limitations of antibiotic treatment, the development of new preventative and therapeutic interventions is crucial. Although clinical trials could be used to test the efficacy of new medical countermeasures (MCMs), the high mortality rates associated with melioidosis raises significant ethical issues concerning treating individuals with new compounds with unknown efficacies. The US Food and Drug Administration (FDA) has formulated a set of guidelines for the licensure of new MCMs to treat diseases in which it would be unethical to test the efficacy of these drugs in humans. The FDA “Animal Rule” 21 CFR 314 calls for consistent, well-characterized B. pseudomallei strains to be used as challenge material in animal models. In order to facilitate the efficacy testing of new MCMs for melioidosis using animal models, we intend to develop a well-characterized panel of strains for use. This panel will comprise of strains that were isolated from human cases, have a low passage history, are virulent in animal models, and are well-characterized phenotypically and genotypically. We have reviewed published and unpublished data on various B. pseudomallei strains to establish an objective method for selecting the strains to be included in the panel of B. pseudomallei strains with attention to five categories: animal infection models, genetic characterization, clinical and passage history, and availability of the strain to the research community. We identified 109 strains with data in at least one of the five categories, scored each strain based on the gathered data and identified six strains as candidate for a B. pseudomallei strain panel. PMID:23057010

An objective approach for Burkholderia pseudomallei strain selection as challenge material for medical countermeasures efficacy testing.

PubMed

Van Zandt, Kristopher E; Tuanyok, Apichai; Keim, Paul S; Warren, Richard L; Gelhaus, H Carl

2012-01-01

Burkholderia pseudomallei is the causative agent of melioidosis, a rare disease of biodefense concern with high mortality and extreme difficulty in treatment. No human vaccines are available that protect against B. pseudomallei infection, and with the current limitations of antibiotic treatment, the development of new preventative and therapeutic interventions is crucial. Although clinical trials could be used to test the efficacy of new medical countermeasures (MCMs), the high mortality rates associated with melioidosis raises significant ethical issues concerning treating individuals with new compounds with unknown efficacies. The US Food and Drug Administration (FDA) has formulated a set of guidelines for the licensure of new MCMs to treat diseases in which it would be unethical to test the efficacy of these drugs in humans. The FDA "Animal Rule" 21 CFR 314 calls for consistent, well-characterized B. pseudomallei strains to be used as challenge material in animal models. In order to facilitate the efficacy testing of new MCMs for melioidosis using animal models, we intend to develop a well-characterized panel of strains for use. This panel will comprise of strains that were isolated from human cases, have a low passage history, are virulent in animal models, and are well-characterized phenotypically and genotypically. We have reviewed published and unpublished data on various B. pseudomallei strains to establish an objective method for selecting the strains to be included in the panel of B. pseudomallei strains with attention to five categories: animal infection models, genetic characterization, clinical and passage history, and availability of the strain to the research community. We identified 109 strains with data in at least one of the five categories, scored each strain based on the gathered data and identified six strains as candidate for a B. pseudomallei strain panel.

Characterizing convective cold pools: Characterizing Convective Cold Pools

DOE PAGES

Drager, Aryeh J.; van den Heever, Susan C.

2017-05-09

Cold pools produced by convective storms play an important role in Earth's climate system. However, a common framework does not exist for objectively identifying convective cold pools in observations and models. The present study investigates convective cold pools within a simulation of tropical continental convection that uses a cloud-resolving model with a coupled land-surface model. Multiple variables are assessed for their potential in identifying convective cold pool boundaries, and a novel technique is developed and tested for identifying and tracking cold pools in numerical model simulations. This algorithm is based on surface rainfall rates and radial gradients in the densitymore » potential temperature field. The algorithm successfully identifies near-surface cold pool boundaries and is able to distinguish between connected cold pools. Once cold pools have been identified and tracked, composites of cold pool evolution are then constructed, and average cold pool properties are investigated. Wet patches are found to develop within the centers of cold pools where the ground has been soaked with rainwater. These wet patches help to maintain cool surface temperatures and reduce cold pool dissipation, which has implications for the development of subsequent convection.« less

Characterizing convective cold pools: Characterizing Convective Cold Pools

DOE Office of Scientific and Technical Information (OSTI.GOV)

Drager, Aryeh J.; van den Heever, Susan C.

Cold pools produced by convective storms play an important role in Earth's climate system. However, a common framework does not exist for objectively identifying convective cold pools in observations and models. The present study investigates convective cold pools within a simulation of tropical continental convection that uses a cloud-resolving model with a coupled land-surface model. Multiple variables are assessed for their potential in identifying convective cold pool boundaries, and a novel technique is developed and tested for identifying and tracking cold pools in numerical model simulations. This algorithm is based on surface rainfall rates and radial gradients in the densitymore » potential temperature field. The algorithm successfully identifies near-surface cold pool boundaries and is able to distinguish between connected cold pools. Once cold pools have been identified and tracked, composites of cold pool evolution are then constructed, and average cold pool properties are investigated. Wet patches are found to develop within the centers of cold pools where the ground has been soaked with rainwater. These wet patches help to maintain cool surface temperatures and reduce cold pool dissipation, which has implications for the development of subsequent convection.« less

Characterization of modulated time-of-flight range image sensors

NASA Astrophysics Data System (ADS)

Payne, Andrew D.; Dorrington, Adrian A.; Cree, Michael J.; Carnegie, Dale A.

2009-01-01

A number of full field image sensors have been developed that are capable of simultaneously measuring intensity and distance (range) for every pixel in a given scene using an indirect time-of-flight measurement technique. A light source is intensity modulated at a frequency between 10-100 MHz, and an image sensor is modulated at the same frequency, synchronously sampling light reflected from objects in the scene (homodyne detection). The time of flight is manifested as a phase shift in the illumination modulation envelope, which can be determined from the sampled data simultaneously for each pixel in the scene. This paper presents a method of characterizing the high frequency modulation response of these image sensors, using a pico-second laser pulser. The characterization results allow the optimal operating parameters, such as the modulation frequency, to be identified in order to maximize the range measurement precision for a given sensor. A number of potential sources of error exist when using these sensors, including deficiencies in the modulation waveform shape, duty cycle, or phase, resulting in contamination of the resultant range data. From the characterization data these parameters can be identified and compensated for by modifying the sensor hardware or through post processing of the acquired range measurements.

First Molecular Characterization of Hypoderma actaeon in Cattle and Red Deer (Cervus elaphus) in Portugal.

PubMed

Ahmed, Haroon; Sousa, Sérgio Ramalho; Simsek, Sami; Anastácio, Sofia; Kilinc, Seyma Gunyakti

2017-12-01

Hypoderma spp. larvae cause subcutaneous myiasis in several animal species. The objective of the present investigation was to identify and characterize morphologically and molecularly the larvae of Hypoderma spp. collected from cattle (Bos taurus taurus) and red deer (Cervus elaphus) in the district of Castelo Branco, Portugal. For this purpose, a total of 8 larvae were collected from cattle (n=2) and red deer (n=6). After morphological identification of Hypoderma spp. larvae, molecular characterization was based on PCR-RFLP and mitochondrial CO1 gene sequence analysis. All larvae were morphologically characterized as the third instar larvae (L3) of H. actaeon. Two restriction enzymes were used for molecular identification of the larvae. TaqI restriction enzyme was not able to cut H. actaeon. However, MboII restriction enzyme differentiated Hypoderma species showing 210 and 450 bp bands in H. actaeon. Furthermore, according to the alignment of the mt-CO1 gene sequences of Hypoderma species and to PCR-RFLP findings, all the identified Hypoderma larvae were confirmed as H. actaeon. This is the first report of identification of Hypoderma spp. (Diptera; Oestridae) from cattle and red deer in Portugal, based on morphological and molecular analyses.

Elementary Reactions and Their Role in Gas-Phase Prebiotic Chemistry

PubMed Central

Balucani, Nadia

2009-01-01

The formation of complex organic molecules in a reactor filled with gaseous mixtures possibly reproducing the primitive terrestrial atmosphere and ocean demonstrated more than 50 years ago that inorganic synthesis of prebiotic molecules is possible, provided that some form of energy is provided to the system. After that groundbreaking experiment, gas-phase prebiotic molecules have been observed in a wide variety of extraterrestrial objects (including interstellar clouds, comets and planetary atmospheres) where the physical conditions vary widely. A thorough characterization of the chemical evolution of those objects relies on a multi-disciplinary approach: 1) observations allow us to identify the molecules and their number densities as they are nowadays; 2) the chemistry which lies behind their formation starting from atoms and simple molecules is accounted for by complex reaction networks; 3) for a realistic modeling of such networks, a number of experimental parameters are needed and, therefore, the relevant molecular processes should be fully characterized in laboratory experiments. A survey of the available literature reveals, however, that much information is still lacking if it is true that only a small percentage of the elementary reactions considered in the models have been characterized in laboratory experiments. New experimental approaches to characterize the relevant elementary reactions in laboratory are presented and the implications of the results are discussed. PMID:19564951

Calibration of a system to collect visible-light polarization data for classification of geosynchronous satellites

NASA Astrophysics Data System (ADS)

Speicher, Andy; Matin, Mohammad; Tippets, Roger; Chun, Francis

2014-09-01

In order to protect critical military and commercial space assets, the United States Space Surveillance Network must have the ability to positively identify and characterize all space objects. Unfortunately, positive identification and characterization of space objects is a manual and labor intensive process today since even large telescopes cannot provide resolved images of most space objects. The objective of this study was to calibrate a system to exploit the optical signature of unresolved geosynchronous satellite images by collecting polarization data in the visible wavelengths for the purpose of revealing discriminating features. These features may lead to positive identification or classification of each satellite. The system was calibrated with an algorithm and process that takes raw observation data from a two-channel polarimeter and converts it to Stokes parameters S0 and S1. This instrumentation is a new asset for the United States Air Force Academy (USAFA) Department of Physics and consists of one 20-inch Ritchey-Chretien telescope and a dual focal plane system fed with a polarizing beam splitter. This study calibrated the system and collected preliminary polarization data on five geosynchronous satellites to validate performance. Preliminary data revealed that each of the five satellites had a different polarization signature that could potentially lead to identification in future studies.

VEGA Launch Vehicle Dynamic Environment: Flight Experience and Qualification Status

NASA Astrophysics Data System (ADS)

Di Trapani, C.; Fotino, D.; Mastrella, E.; Bartoccini, D.; Bonnet, M.

2014-06-01

VEGA Launch Vehicle (LV) during flight is equipped with more than 400 sensors (pressure transducers, accelerometers, microphones, strain gauges...) aimed to catch the physical phenomena occurring during the mission. Main objective of these sensors is to verify that the flight conditions are compliant with the launch vehicle and satellite qualification status and to characterize the phenomena that occur during flight. During VEGA development, several test campaigns have been performed in order to characterize its dynamic environment and identify the worst case conditions, but only with the flight data analysis is possible to confirm the worst cases identified and check the compliance of the operative life conditions with the components qualification status.Scope of the present paper is to show a comparison of the sinusoidal dynamic phenomena that occurred during VEGA first and second flight and give a summary of the launch vehicle qualification status.

Characterization of phenolic compounds in Brazilian pepper (Schinus terebinthifolius Raddi) exocarp.

PubMed

Feuereisen, Michelle M; Hoppe, Julia; Zimmermann, Benno F; Weber, Fabian; Schulze-Kaysers, Nadine; Schieber, Andreas

2014-07-02

The objective of this study was to characterize the phenolic composition of Brazilian pepper (Schinus terebinthifolius Raddi) exocarp extract. Using UHPLC-DAD-MS/MS analysis, four anthocyanins, three biflavonoids, gallic acid, and two types of hydrolyzable tannins (galloyl glucoses, galloyl shikimic acids) were tentatively identified. The structure of the so far unknown 7-O-methylpelargonidin 3-O-β-D-galactopyranoside was elucidated by 2D NMR. Within the group of gallotannins, galloyl shikimic acids with uncommon degrees of galloylation (tetra- to hexagalloyl shikimic acids) were detected. Among the biflavonoids, I3',II8-biapigenin (amentoflavone), I6,II8-biapigenin (agathisflavone), and II-2,3-dihydro-I3',II8-biapigenin were identified, which have already been described for Anacardiaceae. From the results of the present study together with previous findings on the phenolic profile of other Anacardiaceae plants, it is concluded that 7-methoxylated flavonoids are a chemotaxonomic trait frequently found in this family.

Antimicrobial and anti-inflammatory activity of switchgrass-derived extractives

DOE Office of Scientific and Technical Information (OSTI.GOV)

Labbe, Nicole; Ownley, Bonnie H.; Gwinn, Kimberly D.

Switchgrass is an increasingly important biofuel crop, but knowledge of switchgrass fungal pathogens is not extensive. The purpose of this research was to identify the fungal pathogens that decrease crop yield of switchgrass grown in Tennessee and to investigate a potential sustainable disease management strategy from a value-added by-product of the switchgrass biofuel conversion process. The specific objectives were 1) to identify and characterize prevalent fungal pathogens of switchgrass in Tennessee, 2) assess switchgrass seed produced in the United States for seedborne fungal pathogens, and 3) evaluate switchgrass extractives for antimicrobial activity against plant pathogens.

A study of environmental characterization of conventional and advanced aluminum alloys for selection and design. Phase 1: Literature review

NASA Technical Reports Server (NTRS)

Sprowls, D. O.

1984-01-01

A review of the literature is presented with the objectives of identifying relationships between various accelerated stress corrosion testing techniques, and for determining the combination of test methods best suited to selection and design of high strength aluminum alloys. The following areas are reviewed: status of stress-corrosion test standards, the influence of mechanical and environmental factors on stress corrosion testing, correlation of accelerated test data with in-service experience, and procedures used to avoid stress corrosion problems in service. Promising areas for further work are identified.

SiN sub x passivation of silicon surfaces

NASA Technical Reports Server (NTRS)

Olsen, L. C.

1986-01-01

The objectives were to perform surface characterization of high efficiency n+/p and p+/n silicon cells, to relate surface density to substrate dopant concentration, and to identify dominant current loss mechanisms in high efficiency cells. The approach was to measure density of states on homogeneously doped substrates with high frequency C-V and Al/SiN sub x/Si structures; to investigate density of states and photoresponse of high efficiency N+/P and P+/N cells; and to conduct I-V-T studies to identify current loss nechanisms in high efficiency cells. Results are given in tables and graphs.

Automating Network Node Behavior Characterization by Mining Communication Patterns

DOE Office of Scientific and Technical Information (OSTI.GOV)

Carroll, Thomas E.; Chikkagoudar, Satish; Arthur-Durett, Kristine M.

Enterprise networks of scale are complex, dynamic computing environments that respond to evolv- ing business objectives and requirements. Characteriz- ing system behaviors in these environments is essential for network management and cyber security operations. Characterization of system’s communication is typical and is supported using network flow information (NetFlow). Related work has characterized behavior using theoretical graph metrics; results are often difficult to interpret by enterprise staff. We propose a different approach, where flow information is mapped to sets of tags that contextualize the data in terms of network principals and enterprise concepts. Frequent patterns are then extracted and are expressedmore » as behaviors. Behaviors can be com- pared, identifying systems expressing similar behaviors. We evaluate the approach using flow information collected by a third party.« less

Fungal endophytes characterization from four species of Diplazium Swartz

NASA Astrophysics Data System (ADS)

Affina-Eliya, A. A.; Noraini, T.; Nazlina, I.; Ruzi, A. R.

2014-09-01

Four species on genus Diplazium namely Diplazium tomentosum, D. sorzogonense, D. asperum and D. accedens of Peninsular Malaysia were studied for presence of fungal endophyte. The objective of this study is to characterize fungal endophytes in the rhizome of four Diplazium species. The rhizome was surface sterilized and incubated to isolate fungal endophytes. Characterization of the colonies was performed by macroscopic morphological, microscopic identification, types of hyphae and mycelium, and spore structure. For isolation that produces spores, the structure of conidiophores and conidia were identified. From this study, four fungal have been isolated and determined as Aspergillus sp. (isolates AE 1), Aspergillus fumigatus (isolates AE 2), Aspergillus versicolor (isolates AE 3) and Verticillium sp. (isolates AE 4). The fungal isolates from this study were classified from the same family Moniliaceae.

Hemicellulases from the ethanologenic thermophile, Thermoanaerobacter ethanolicus and related anaerobic thermophiles. Final report, September 1992--June 1996

DOE Office of Scientific and Technical Information (OSTI.GOV)

Wiegel, J.

1998-09-01

The short term goals of this application were to characterize hemicellulases from anaerobic thermophiles on the biochemical and molecular level to extend the presently limited knowledge of hemicellulases in anaerobic thermophilic bacteria. This objective includes the following tasks: (1) Traditional purification and biochemical/biophysical characterization of xylanases from the newly isolated, slightly alkalitolerant strain NDF190, and the slightly acid-tolerant strain YS485, both with high xylanolytic activities, and of the 4-O-methyl glucuronidase and arabinosidase from strain NDF190 and the acetyl (xylan) esterase from T. ethanolicus. This also includes determining the N-terminal sequences and obtaining gene probes. (2) Elucidation of the regulation ofmore » hemicellulolytic enzymes in anaerobic thermophiles. (3) To clone into E. coli and identify the multiplicity of the enzymes involved in hemicellulose degradation by T. ethanolicus and other suitable organisms. (4) To purify and characterize the recombinant enzymes with the goal of identifying the best enzymes for cloning into the ethanologenic T. ethanolicus to obtain an optimized hemicellulose utilization by this bacterium.« less

Hemicellulases from the ethanologenic thermophile Thermoanaerobacter ethanolicus and related anaerobic thermophiles. Final report, September 1992--June 1996

DOE Office of Scientific and Technical Information (OSTI.GOV)

Wiegel, J.

1998-05-01

The SHORT TERM GOALS of this application were to characterize hemicellulases from anaerobic thermophiles on the biochemical and molecular level to extend the presently limited knowledge of hemicellulases in anaerobic thermophilic bacteria. This objective includes the following TASKS: (1) Traditional purification and biochemical/biophysical characterization of xylanases from the newly isolated, slightly alkalitolerant strain NDF190, and the slightly acid-tolerant strain YS485, both with high xylanolytic activities, and of the 4-0-methyl glucuronidase and arabinosidase from strain NDF190 and the acetyl (xylan) esterase from T. ethanolicus. This also includes determining the N-terminal sequences and obtaining gene probes. (2) Elucidation of the regulation ofmore » hemicellulolytic enzymes in anaerobic thermophiles. (3) To clone into E. coli and identify the multiplicity of the enzymes involved in hemicellulose degradation by T. ethanolicus and other suitable organisms. (4) To purify and characterize the recombinant enzymes with the goal of identifying the best enzymes for cloning into the ethanologenic T. ethanolicus to obtain an optimized hemicellulose utilization by this bacterium (one of our long term goals).« less

A science-based, watershed strategy to support effective remediation of abandoned mine lands

USGS Publications Warehouse

Buxton, Herbert T.; Nimick, David A.; Von Guerard, Paul; Church, Stan E.; Frazier, Ann G.; Gray, John R.; Lipin, Bruce R.; Marsh, Sherman P.; Woodward, Daniel F.; Kimball, Briant A.; Finger, Susan E.; Ischinger, Lee S.; Fordham, John C.; Power, Martha S.; Bunch, Christine M.; Jones, John W.

1997-01-01

A U.S. Geological Survey Abandoned Mine Lands Initiative will develop a strategy for gathering and communicating the scientific information needed to formulate effective and cost-efficient remediation of abandoned mine lands. A watershed approach will identify, characterize, and remediate contaminated sites that have the most profound effect on water and ecosystem quality within a watershed. The Initiative will be conducted during 1997 through 2001 in two pilot watersheds, the Upper Animas River watershed in Colorado and the Boulder River watershed in Montana. Initiative efforts are being coordinated with the U.S. Forest Service, Bureau of Land Management, National Park Service, and other stakeholders which are using the resulting scientific information to design and implement remediation activities. The Initiative has the following eight objective-oriented components: estimate background (pre-mining) conditions; define baseline (current) conditions; identify target sites (major contaminant sources); characterize target sites and processes affecting contaminant dispersal; characterize ecosystem health and controlling processes at target sites; develop remediation goals and monitoring network; provide an integrated, quality-assured and accessible data network; and document lessons learned for future applications of the watershed approach.

The I3I Model; Identifying Cultural Determinants of Information Sharing via C2 Information Technologies

DTIC Science & Technology

2009-06-01

Individualist cultures represent loose ties between individuals where the interests of individuals prevail over the interests of the group and the...independence of individuals is emphasized. Individual accomplishments are valued whereas in collectivist cultures the group’s well being and common...goals and objectives are valued more. Collectivist cultures are characterized by tight social networks in which individuals strongly distinguish

Connecting West Virginia fee-fishing businesses with the larger tourism market through the development of tourism package

Treesearch

Zongxiang Mei; Chad Pierskalla; Michael Shuett

2007-01-01

Thirty-five or more fee-fishing businesses in West Virginia are often characterized as small businesses, and they could benefit from connecting with larger travel packages that are more likely to attract out-of state anglers. The objectives of this study are to: (1) identify mini-market segments based on fee-fishing experiences; (2) examine how fee-fishing mini-markets...

Automated single-trial assessment of laser-evoked potentials as an objective functional diagnostic tool for the nociceptive system.

PubMed

Hatem, S M; Hu, L; Ragé, M; Gierasimowicz, A; Plaghki, L; Bouhassira, D; Attal, N; Iannetti, G D; Mouraux, A

2012-12-01

To assess the clinical usefulness of an automated analysis of event-related potentials (ERPs). Nociceptive laser-evoked potentials (LEPs) and non-nociceptive somatosensory electrically-evoked potentials (SEPs) were recorded in 37 patients with syringomyelia and 21 controls. LEP and SEP peak amplitudes and latencies were estimated using a single-trial automated approach based on time-frequency wavelet filtering and multiple linear regression, as well as a conventional approach based on visual inspection. The amplitudes and latencies of normal and abnormal LEP and SEP peaks were identified reliably using both approaches, with similar sensitivity and specificity. Because the automated approach provided an unbiased solution to account for average waveforms where no ERP could be identified visually, it revealed significant differences between patients and controls that were not revealed using the visual approach. The automated analysis of ERPs characterized reliably and objectively LEP and SEP waveforms in patients. The automated single-trial analysis can be used to characterize normal and abnormal ERPs with a similar sensitivity and specificity as visual inspection. While this does not justify its use in a routine clinical setting, the technique could be useful to avoid observer-dependent biases in clinical research. Copyright © 2012 International Federation of Clinical Neurophysiology. Published by Elsevier Ireland Ltd. All rights reserved.

Assessing invasive plant infestation in freshwater wetlands

NASA Astrophysics Data System (ADS)

Torbick, Nathan M.

Recent shifts in wetland ecosystem management goals have directed efforts toward measuring ecological integrity, rather than only using physical and chemical measures of ecosystems as health indicators. Invasive species pose one of the largest threats to wetlands integrity. Resource managers can benefit from improved methods for identifying invasive plant species, assessing infestation, and monitoring control measures. The utilization of advanced remote sensing tools for species-level mapping has been increasing and techniques need to be explored for identifying species of interest and characterizing infestation. The overarching goal of this research was to develop monitoring technologies to map invasive plants and quantify wetland infestation. The first field-level objective was to characterize absorption and reflectance features and assess processing techniques for separating wetland species. The second field-level objective was to evaluate the abilities of a shape filter to identify wetland invasive plant species. The first landscape-level objective was to classify hyperspectral imagery in order to identify invasives of interest. The second landscape-level objective was to quantify infestation within the study area. Field-level hyperspectral data (350-2500nm) were collected for twenty-two wetland plant species in a wetland located in the lower Muskegon River watershed in Michigan, USA. The Jeffries-Matusita distance measure, continuum removal, and a shape-filter were applied to hyperspectral species reflectance data to characterize spectral features. Generally, continuum removal decreased separation distance for the invasive species of interest. Using the shape-filter, Lythrum salicaria, Phragmites australis, and Typha latifolia possessed maximum separation (distinguished from other species) at the near-infrared edge (700nm) and water absorption region (1350nm), the near-infrared down slope (1000 and 1100nm), and the visible/chlorophyll absorption region (500nm) and near-infrared edge (650nm), respectively. Airborne hyperspectral imagery was classified using a two-step approach in order to obtain an optimal map (overall accuracy ˜ 70%). Information in the near-infrared enabled relatively accurate classification for Phragmites australis using the Spectral Angle Mapper algorithm and image-derived training, while Typha latifolia signatures possessed high spectral overlap and required ISODATA clustering techniques. Landscape pattern metrics relate infestation to disturbances and hydrological controls. The highest levels of infestation and infestation patterns coincide with the most substantial levels of hydrological modifications indicating human disturbances are correlated with Typha and Phragmites percentages in the landscape. Overall the approach was successful and increased the level of information ultimately desired by decision makers. The rapidly advancing field of wetland remote sensing science can obtain more meaningful information from hyperspectral imagery; however, the data are challenging to work with and only the most precisely calibrated datasets will provide utility. Combining these data with traditional wetland assessment techniques can substantially advanced goals of preserving and restoring wetland ecosystem integrity.

Three-Dimensional Object Recognition and Registration for Robotic Grasping Systems Using a Modified Viewpoint Feature Histogram

PubMed Central

Chen, Chin-Sheng; Chen, Po-Chun; Hsu, Chih-Ming

2016-01-01

This paper presents a novel 3D feature descriptor for object recognition and to identify poses when there are six-degrees-of-freedom for mobile manipulation and grasping applications. Firstly, a Microsoft Kinect sensor is used to capture 3D point cloud data. A viewpoint feature histogram (VFH) descriptor for the 3D point cloud data then encodes the geometry and viewpoint, so an object can be simultaneously recognized and registered in a stable pose and the information is stored in a database. The VFH is robust to a large degree of surface noise and missing depth information so it is reliable for stereo data. However, the pose estimation for an object fails when the object is placed symmetrically to the viewpoint. To overcome this problem, this study proposes a modified viewpoint feature histogram (MVFH) descriptor that consists of two parts: a surface shape component that comprises an extended fast point feature histogram and an extended viewpoint direction component. The MVFH descriptor characterizes an object’s pose and enhances the system’s ability to identify objects with mirrored poses. Finally, the refined pose is further estimated using an iterative closest point when the object has been recognized and the pose roughly estimated by the MVFH descriptor and it has been registered on a database. The estimation results demonstrate that the MVFH feature descriptor allows more accurate pose estimation. The experiments also show that the proposed method can be applied in vision-guided robotic grasping systems. PMID:27886080

Changes in Youth’s Experiences of Child Maltreatment Across Developmental Periods in the LONGSCAN consortium

PubMed Central

Villodas, Miguel T.; Litrownik, Alan J.; Thompson, Richard; Roesch, Scott C.; English, Diana J.; Dubowitz, Howard; Kotch, Jonathan B.; Runyan, Desmond K.

2013-01-01

Objective The present study applied person-centered data analytic techniques to identify groups of youth with allegations for combinations of maltreatment types during preschool, early and late childhood. Method Latent Class Analyses were conducted using officially reported child maltreatment data for five types of maltreatment (i.e., failure-to-provide and lack-of-supervision neglect, and physical, sexual and emotional abuse) from 788 youth in a large prospective study during preschool, early, and late childhood. Results Three similar classes were identified during preschool and early childhood, characterized by no maltreatment allegations, allegations for neglect and emotional maltreatment, and allegations for all maltreatment types. During late childhood, four classes were identified characterized by no maltreatment allegations, mixed patterns of maltreatment allegations, physical and emotional abuse allegations, and allegations for all maltreatment types. Youth in maltreated classes were more likely to be re-victimized during subsequent developmental periods, often by similar maltreatment combinations. Youth in maltreated classes characterized by physically violent maltreatment types had higher Externalizing and Total behavior problems at each age. Conclusions These findings confirm the vulnerability of maltreated youth to re-victimization, particularly by similar combinations of maltreatment. They also indicate that youth’s susceptibilities to specific forms of maltreatment may vary across developmental periods. PMID:24999441

Merging analytic and empirical GEO debris synchronization dynamics

NASA Astrophysics Data System (ADS)

Anderson, Paul V.; McKnight, Darren S.; Di Pentino, Frank; Schaub, Hanspeter

2016-09-01

The motion of abandoned satellites near the geostationary (GEO) region has been extensively studied, modeled, and compared to the motion of station-kept, operational satellites, providing insights into the evolution of uncontrolled orbits at GEO. Analytic developments produced a family of curves represented in the ascending node versus inclination space describing the long-term precession of the orbit plane at GEO, and forecasted the clustering of objects at the geopotential wells. However, recent investigations were undertaken to characterize apparent anomalistic behavior of GEO objects and classification of objects into related families. This paper provides a unifying summary of early bottom-up analytical theory with more recent top-down operational observations, highlighting the common linkage between these dimensions of GEO object behavior. This paper also identifies the relevance of these patterns of life tendencies for future operations at and near GEO, and discusses the long-term implications of these patterns of life for space situational awareness activities in this regime.

[Object-oriented remote sensing image classification in epidemiological studies of visceral leishmaniasis in urban areas].

PubMed

Almeida, Andréa Sobral de; Werneck, Guilherme Loureiro; Resendes, Ana Paula da Costa

2014-08-01

This study explored the use of object-oriented classification of remote sensing imagery in epidemiological studies of visceral leishmaniasis (VL) in urban areas. To obtain temperature and environmental information, an object-oriented classification approach was applied to Landsat 5 TM scenes from the city of Teresina, Piauí State, Brazil. For 1993-1996, VL incidence rates correlated positively with census tracts covered by dense vegetation, grass/pasture, and bare soil and negatively with areas covered by water and densely populated areas. In 2001-2006, positive correlations were found with dense vegetation, grass/pasture, bare soil, and densely populated areas and negative correlations with occupied urban areas with some vegetation. Land surface temperature correlated negatively with VL incidence in both periods. Object-oriented classification can be useful to characterize landscape features associated with VL in urban areas and to help identify risk areas in order to prioritize interventions.

Cost-effectiveness methodology for computer systems selection

NASA Technical Reports Server (NTRS)

Vallone, A.; Bajaj, K. S.

1980-01-01

A new approach to the problem of selecting a computer system design has been developed. The purpose of this methodology is to identify a system design that is capable of fulfilling system objectives in the most economical way. The methodology characterizes each system design by the cost of the system life cycle and by the system's effectiveness in reaching objectives. Cost is measured by a 'system cost index' derived from an analysis of all expenditures and possible revenues over the system life cycle. Effectiveness is measured by a 'system utility index' obtained by combining the impact that each selection factor has on the system objectives and it is assessed through a 'utility curve'. A preestablished algorithm combines cost and utility and provides a ranking of the alternative system designs from which the 'best' design is selected.

Characterization of urban solid waste in Chihuahua, Mexico.

PubMed

Gomez, Guadalupe; Meneses, Montserrat; Ballinas, Lourdes; Castells, Francesc

2008-12-01

The characterization of urban solid waste generation is fundamental for adequate decision making in the management strategy of urban solid waste in a city. The objective of this study is to characterize the waste generated in the households of Chihuahua city, and to compare the results obtained in areas of the city with three different socioeconomic levels. In order to identify the different socioeconomic trends in waste generation and characterization, 560 samples of solid waste were collected during 1 week from 80 households in Chihuahua and were hand sorted and classified into 15 weighted fractions. The average waste generation in Chihuahua calculated in this study was 0.676 kg per capita per day in April 2006. The main fractions were: organic (48%), paper (16%) and plastic (12%). Results show an increased waste generation associated with the socioeconomic level. The characterization in amount and composition of urban waste is the first step needed for the successful implementation of an integral waste management system.

Can CanMEDS competencies be developed in medical school anatomy laboratories? A literature review

PubMed Central

Ramnanan, Christopher J.

2017-01-01

Objectives The purpose of this literature review was to identify potential ways in which undergraduate medical anatomy education may be relevant to the CanMEDS Roles, a competency-based framework used throughout Canadian medical training. Methods A scoping review of medical education literature was conducted in March 2017 for English language publications that included key words related to anatomy education and to key competencies formally described for each of the Roles in the CanMEDS 2015 framework. Indicated benefits were then collated, characterized, and synthesized for each CanMEDS Role. Results There were 71 studies identified describing original findings. Perceived benefits of anatomy education were most often identified for competencies related to the Medical Expert Role. Multiple studies also cited benefits related to the Scholar, Professional and Collaborator Roles. There was a lack of literature related to the Health Advocate, Communicator, and Leader Roles. The majority of benefits defined in the literature were limited to student perceptions rather than objectively measured outcomes. Conclusions There is some evidence to suggest that anatomy education can facilitate the development of core competencies related to several CanMEDS Roles, outside of simply developing medical knowledge in the Medical Expert Role. Future studies need to develop methods to objectively assess outcomes related to these competencies. PMID:28650843

Grouping in object recognition: the role of a Gestalt law in letter identification.

PubMed

Pelli, Denis G; Majaj, Najib J; Raizman, Noah; Christian, Christopher J; Kim, Edward; Palomares, Melanie C

2009-02-01

The Gestalt psychologists reported a set of laws describing how vision groups elements to recognize objects. The Gestalt laws "prescribe for us what we are to recognize 'as one thing'" (Kohler, 1920). Were they right? Does object recognition involve grouping? Tests of the laws of grouping have been favourable, but mostly assessed only detection, not identification, of the compound object. The grouping of elements seen in the detection experiments with lattices and "snakes in the grass" is compelling, but falls far short of the vivid everyday experience of recognizing a familiar, meaningful, named thing, which mediates the ordinary identification of an object. Thus, after nearly a century, there is hardly any evidence that grouping plays a role in ordinary object recognition. To assess grouping in object recognition, we made letters out of grating patches and measured threshold contrast for identifying these letters in visual noise as a function of perturbation of grating orientation, phase, and offset. We define a new measure, "wiggle", to characterize the degree to which these various perturbations violate the Gestalt law of good continuation. We find that efficiency for letter identification is inversely proportional to wiggle and is wholly determined by wiggle, independent of how the wiggle was produced. Thus the effects of three different kinds of shape perturbation on letter identifiability are predicted by a single measure of goodness of continuation. This shows that letter identification obeys the Gestalt law of good continuation and may be the first confirmation of the original Gestalt claim that object recognition involves grouping.

Grouping in object recognition: The role of a Gestalt law in letter identification

PubMed Central

Pelli, Denis G.; Majaj, Najib J.; Raizman, Noah; Christian, Christopher J.; Kim, Edward; Palomares, Melanie C.

2009-01-01

The Gestalt psychologists reported a set of laws describing how vision groups elements to recognize objects. The Gestalt laws “prescribe for us what we are to recognize ‘as one thing’” (Köhler, 1920). Were they right? Does object recognition involve grouping? Tests of the laws of grouping have been favourable, but mostly assessed only detection, not identification, of the compound object. The grouping of elements seen in the detection experiments with lattices and “snakes in the grass” is compelling, but falls far short of the vivid everyday experience of recognizing a familiar, meaningful, named thing, which mediates the ordinary identification of an object. Thus, after nearly a century, there is hardly any evidence that grouping plays a role in ordinary object recognition. To assess grouping in object recognition, we made letters out of grating patches and measured threshold contrast for identifying these letters in visual noise as a function of perturbation of grating orientation, phase, and offset. We define a new measure, “wiggle”, to characterize the degree to which these various perturbations violate the Gestalt law of good continuation. We find that efficiency for letter identification is inversely proportional to wiggle and is wholly determined by wiggle, independent of how the wiggle was produced. Thus the effects of three different kinds of shape perturbation on letter identifiability are predicted by a single measure of goodness of continuation. This shows that letter identification obeys the Gestalt law of good continuation and may be the first confirmation of the original Gestalt claim that object recognition involves grouping. PMID:19424881

A cooling flow cluster at redshift Z = 0.2

NASA Astrophysics Data System (ADS)

Wolter, Anna; Schild, R.; Gioia, I. M.; Maccacaro, T.; Morris, S. L.; Nesci, R.; Perola, G. C.

The cluster of galaxies 1E0839.9 + 2938, discovered in X-ray observations by Nesci et al. (1988), is characterized on the basis of VLA 6-cm radio observations, Whipple Observatory CCD photometry, and spectroscopic observations obtained with the Multiple Mirror Telescope and the 88-inch University of Hawaii Telescope at Mauna Kea. The data are presented in tables, maps, and sample images and spectra and briefly characterized. The bright X-ray object is identified with a cluster at redshift z = 0.195; its central galaxy has radio emission of 1.1 x 10 exp 24 W/Hz as well as strong optical line emission which is not restricted to its nucleus. It is concluded that 1E0839.9 + 2938 is a cooling-flow cluster similar to 3C295 (found at z = 0.461 by Henry et al., 1986). The need for space observations (by Rosat or the AXAF) to determine the object's X-ray luminosity distribution is indicated.

A cooling flow cluster at redshift z = 0.2

NASA Technical Reports Server (NTRS)

Wolter, Anna; Schild, R.; Gioia, I. M.; Maccacaro, T.; Morris, S. L.; Nesci, R.; Perola, G. C.

1990-01-01

The cluster of galaxies 1E0839.9 + 2938, discovered in X-ray observations by Nesci et al. (1988), is characterized on the basis of VLA 6-cm radio observations, Whipple Observatory CCD photometry, and spectroscopic observations obtained with the Multiple Mirror Telescope and the 88-inch University of Hawaii Telescope at Mauna Kea. The data are presented in tables, maps, and sample images and spectra and briefly characterized. The bright X-ray object is identified with a cluster at redshift z = 0.195; its central galaxy has radio emission of 1.1 x 10 exp 24 W/Hz as well as strong optical line emission which is not restricted to its nucleus. It is concluded that 1E0839.9 + 2938 is a cooling-flow cluster similar to 3C295 (found at z = 0.461 by Henry et al., 1986). The need for space observations (by Rosat or the AXAF) to determine the object's X-ray luminosity distribution is indicated.

Culture and context: East Asian American and European American differences in P3 event-related potentials and self-construal.

PubMed

Lewis, Richard S; Goto, Sharon G; Kong, Lauren L

2008-05-01

Research has demonstrated differences in social and cognitive processes between East Asians and European Americans. Whereas East Asians have been characterized as being more sensitive to situational context and attending more to the perceptual field, European Americans have been characterized as being more focused on the object and being more field independent. The goal of the present experiment was to investigate differences in neural responses to target objects and stimulus context between East Asian Americans and European Americans using a three-stimulus novelty P3 event-related potential design. As hypothesized, European Americans displayed relatively greater target P3 amplitudes, indexing attention to target events, whereas East Asian Americans displayed relatively greater novelty P3 amplitudes, indexing attention to contextually deviant events. Furthermore, the authors found that interdependent self-construal mediated the relationship between culture and the novelty P3. These findings identify a specific pattern of neural activity associated with established cultural differences in contextual sensitivity.

Arecibo Radar Observations of Near-Earth Asteroids

NASA Astrophysics Data System (ADS)

Rivera-Valentin, Edgard G.; Taylor, Patrick A.; Virkki, Anne; Saran Bhiravarasu, Sriram; Venditti, Flaviane; Zambrano-Marin, Luisa Fernanda; Aponte-Hernandez, Betzaida

2017-10-01

The Arecibo S-Band (2.38 GHz, 12.6 cm; 1 MW) planetary radar system at the 305-m William E. Gordon Telescope in Arecibo, Puerto Rico is the most active, most powerful, and most sensitive planetary radar facility in the world. As such, Arecibo is vital for post-discovery characterization and orbital refinement of near-Earth asteroids. Since August 2016, the program has observed 100 near-Earth asteroids (NEAs), of which 38 are classified as potentially hazardous to Earth and 31 are compliant with the NASA Near-Earth Object Human Space Flight Accessible Targets Study (NHATS). Arecibo observations are critical for identifying NEAs that may be on a collision course with Earth in addition to providing detailed physical characterization of the objects themselves in terms of size, shape, spin, and surface properties, which are valuable for assessing impact mitigation strategies. Here, we will present a sampling of the asteroid zoo observed by Arecibo, including press-noted asteroids 2014 JO25 and the (163693) Atira binary system.

Environmental Analysis of Endocrine Disrupting Effects from Hydrocarbon Contaminants in the Ecosystem - Final Report - 09/15/1996 - 09/14/2000

DOE Office of Scientific and Technical Information (OSTI.GOV)

McLachlan, John A.

The three major components of the research included: (a) a biotechnology based screening system to identify potential hormone mimics and antagonists (b) an animal screening system to identify biomarkers of endocrine effects and (c) a literature review to identify compounds at various DOE sites that are potential endocrine disruptors. Species of particular interest in this study were those that can serve as sentinel species (e.g., amphibians) and thus provide early warning signals for more widespread impacts on an ecosystem and its wildlife and human inhabitants. The objective of this basic research is to characterize the potential of common hydrocarbon contaminantsmore » in ecosystems to act as endocrine disruptors. Although the endocrine disrupting effects of contaminants such as dioxin and PCBs have been well characterized in both animals and humans, little is known about the capacities of other hydrocarbon contaminants to act as endocrine disruptors. Results obtained from this research project have provided information on endocrine disrupting contaminants for consideration in DOE's risk analyses for determining clean-up levels and priorities at contaminated DOE sites.« less

Production and chemical characterization of pigments in filamentous fungi.

PubMed

Souza, Patrícia Nirlane da Costa; Grigoletto, Tahuana Luiza Bim; de Moraes, Luiz Alberto Beraldo; Abreu, Lucas M; Guimarães, Luís Henrique Souza; Santos, Cledir; Galvão, Luciano Ribeiro; Cardoso, Patrícia Gomes

2016-01-01

Production of pigments by filamentous fungi is gaining interest owing to their use as food colourants, in cosmetics and textiles, and because of the important biological activities of these compounds. In this context, the objectives of this study were to select pigment-producing fungi, identify these fungi based on internal transcribed spacer sequences, evaluate the growth and pigment production of the selected strains on four different media, and characterize the major coloured metabolites in their extracts. Of the selected fungal strains, eight were identified as Aspergillus sydowii (CML2967), Aspergillus aureolatus (CML2964), Aspergillus keveii (CML2968), Penicillium flavigenum (CML2965), Penicillium chermesinum (CML2966), Epicoccum nigrum (CML2971), Lecanicillium aphanocladii (CML2970) and Fusarium sp. (CML2969). Fungal pigment production was influenced by medium composition. Complex media, such as potato dextrose and malt extract, favoured increased pigment production. The coloured compounds oosporein, orevactaene and dihydrotrichodimerol were identified in extracts of L. aphanocladii (CML2970), E. nigrum (CML2971), and P. flavigenum (CML2965), respectively. These results indicate that the selected fungal strains can serve as novel sources of pigments that have important industrial applications.

Evidence-Based Occupational Hearing Screening I: Modeling the Effects of Real-World Noise Environments on the Likelihood of Effective Speech Communication.

PubMed

Soli, Sigfrid D; Giguère, Christian; Laroche, Chantal; Vaillancourt, Véronique; Dreschler, Wouter A; Rhebergen, Koenraad S; Harkins, Kevin; Ruckstuhl, Mark; Ramulu, Pradeep; Meyers, Lawrence S

The objectives of this study were to (1) identify essential hearing-critical job tasks for public safety and law enforcement personnel; (2) determine the locations and real-world noise environments where these tasks are performed; (3) characterize each noise environment in terms of its impact on the likelihood of effective speech communication, considering the effects of different levels of vocal effort, communication distances, and repetition; and (4) use this characterization to define an objective normative reference for evaluating the ability of individuals to perform essential hearing-critical job tasks in noisy real-world environments. Data from five occupational hearing studies performed over a 17-year period for various public safety agencies were analyzed. In each study, job task analyses by job content experts identified essential hearing-critical tasks and the real-world noise environments where these tasks are performed. These environments were visited, and calibrated recordings of each noise environment were made. The extended speech intelligibility index (ESII) was calculated for each 4-sec interval in each recording. These data, together with the estimated ESII value required for effective speech communication by individuals with normal hearing, allowed the likelihood of effective speech communication in each noise environment for different levels of vocal effort and communication distances to be determined. These likelihoods provide an objective norm-referenced and standardized means of characterizing the predicted impact of real-world noise on the ability to perform essential hearing-critical tasks. A total of 16 noise environments for law enforcement personnel and eight noise environments for corrections personnel were analyzed. Effective speech communication was essential to hearing-critical tasks performed in these environments. Average noise levels, ranged from approximately 70 to 87 dBA in law enforcement environments and 64 to 80 dBA in corrections environments. The likelihood of effective speech communication at communication distances of 0.5 and 1 m was often less than 0.50 for normal vocal effort. Likelihood values often increased to 0.80 or more when raised or loud vocal effort was used. Effective speech communication at and beyond 5 m was often unlikely, regardless of vocal effort. ESII modeling of nonstationary real-world noise environments may prove an objective means of characterizing their impact on the likelihood of effective speech communication. The normative reference provided by these measures predicts the extent to which hearing impairments that increase the ESII value required for effective speech communication also decrease the likelihood of effective speech communication. These predictions may provide an objective evidence-based link between the essential hearing-critical job task requirements of public safety and law enforcement personnel and ESII-based hearing assessment of individuals who seek to perform these jobs.

The Mission Accessible Near-Earth Objects Survey (MANOS): spectroscopy results

NASA Astrophysics Data System (ADS)

Thomas, Cristina A.; Moskovitz, Nicholas; Hinkle, Mary L.; Mommert, Michael; Polishook, David; Thirouin, Audrey; Binzel, Richard; Christensen, Eric J.; DeMeo, Francesca E.; Person, Michael J.; Trilling, David E.; Willman, Mark; Burt, Brian

2016-10-01

The Mission Accessible Near-Earth Object Survey (MANOS) is an ongoing physical characterization survey to build a large, uniform catalog of physical properties including lightcurves and visible wavelength spectroscopy. We will use this catalog to investigate the global properties of the small NEO population and identify individual objects that can be targets of interest for future exploration. To accomplish our goals, MANOS uses a wide variety of telescopes (1-8m) in both the northern and southern hemispheres. We focus on targets that have been recently discovered and operate on a regular cadence of remote and queue observations to enable rapid characterization of small NEOs. Targets for MANOS are selected based on three criteria: mission accessibility, size, and observability. With our resources, we observe 5-10 newly discovered sub-km NEOs per month. MANOS has been operating for three years and we have observed over 500 near-Earth objects in that time.We will present results from the spectroscopy component of the MANOS program. Visible wavelength spectra are obtained using DeVeny on the Discovery Channel Telescope (DCT), Goodman on the Southern Astrophysical Research (SOAR) telescope, and GMOS on Gemini North and South. Over 300 NEO spectra have been obtained during our program. We will present preliminary results from our spectral sample. We will discuss the compositional diversity of the small NEO population and how the observed NEOs compare to the meteorite population.MANOS is funded by the NASA Near-Earth Object Observations program.

Comparative Performance Analysis of Intel Xeon Phi, GPU, and CPU: A Case Study from Microscopy Image Analysis

PubMed Central

Teodoro, George; Kurc, Tahsin; Kong, Jun; Cooper, Lee; Saltz, Joel

2014-01-01

We study and characterize the performance of operations in an important class of applications on GPUs and Many Integrated Core (MIC) architectures. Our work is motivated by applications that analyze low-dimensional spatial datasets captured by high resolution sensors, such as image datasets obtained from whole slide tissue specimens using microscopy scanners. Common operations in these applications involve the detection and extraction of objects (object segmentation), the computation of features of each extracted object (feature computation), and characterization of objects based on these features (object classification). In this work, we have identify the data access and computation patterns of operations in the object segmentation and feature computation categories. We systematically implement and evaluate the performance of these operations on modern CPUs, GPUs, and MIC systems for a microscopy image analysis application. Our results show that the performance on a MIC of operations that perform regular data access is comparable or sometimes better than that on a GPU. On the other hand, GPUs are significantly more efficient than MICs for operations that access data irregularly. This is a result of the low performance of MICs when it comes to random data access. We also have examined the coordinated use of MICs and CPUs. Our experiments show that using a performance aware task strategy for scheduling application operations improves performance about 1.29× over a first-come-first-served strategy. This allows applications to obtain high performance efficiency on CPU-MIC systems - the example application attained an efficiency of 84% on 192 nodes (3072 CPU cores and 192 MICs). PMID:25419088

High Cycle Fatigue (HCF) Science and Technology Program 2002 Annual Report

DTIC Science & Technology

2003-08-01

Turbine Engine Airfoils, Phase I 4.3 Probabilistic Design of Turbine Engine Airfoils, Phase II 4.4 Probabilistic Blade Design System 4.5...XTL17/SE2 7.4 Conclusion 8.0 TEST AND EVALUATION 8.1 Characterization Test Protocol 8.2 Demonstration Test Protocol 8.3 Development of Multi ...transparent and opaque overlays for processing. The objective of the SBIR Phase I program was to identify and evaluate promising methods for

Daily Mood Patterns and Bulimic Behaviors in the Natural Environment

PubMed Central

Crosby, Ross D.; Wonderlich, Stephen A.; Engel, Scott G.; Simonich, Heather; Smyth, Joshua; Mitchell, James E.

2009-01-01

Objective Negative affect has been purported to play an important role in the etiology and maintenance of bulimic behaviors. The objective of this study was to identify daily mood patterns in the natural environment exhibited by individuals with bulimia nervosa and to examine the relationship between these patterns and bulimic behaviors. Method One hundred thirty-three women aged 18–55 meeting DSM-IV criteria for bulimia nervosa were recruited through clinical referrals and community advertisements. Ecological momentary assessment was used to collect multiple ratings of negative affect, binge eating and purging each day for a two-week period using palmtop computers. Latent growth mixture modeling was used to identify daily mood patterns. Results Nine distinct daily mood patterns were identified. The highest rates of binge eating and purging episodes occurred on days characterized by stable high negative affect or increasing negative affect over the course of the day. Conclusions These findings support the conclusion that negative mood states are intimately tied to bulimic behaviors and may in fact precipitate such behavior. PMID:19152874

DOE Office of Scientific and Technical Information (OSTI.GOV)

Sorensen, Christina M.; Ding, Jie; Zhang, Qibin

Objectives: To characterize the lipid profile of individuals with newly diagnosed type 1 diabetes mellitus using LC-MS-based lipidomics and the accurate mass and time (AMT) tag approach. Design and methods: Lipids were extracted from plasma and sera of 10 subjects from the Diabetes Antibody Standardization Program (years 2000-2005) and 10 non-diabetic subjects and analyzed by capillary liquid chromatography coupled with a hybrid ion-trap-Fourier transform ion cyclotron resonance mass spectrometer. Lipids were identified and quantified using the AMT tag approach. Results: Five hundred sixty lipid features differentiated (q < 0.05) diabetic from healthy individuals in a partial least-squares analysis, characterizing ofmore » individuals with recently diagnosed type 1 diabetes mellitus. Conclusions: A lipid profile associated with newly diagnosed type 1 diabetes may aid in further characterization of biochemical pathways involved in lipid regulation or mobilization and lipotoxicity of pancreatic beta-cells.« less

Microbial ecology of deep-water mid-Atlantic canyons

USGS Publications Warehouse

Kellogg, Christina A.

2011-01-01

The research described in this fact sheet will be conducted from 2012 to 2014 as part of the U.S. Geological Survey's DISCOVRE (DIversity, Systematics, and COnnectivity of Vulnerable Reef Ecosystems) Program. This integrated, multidisciplinary effort will be investigating a variety of topics related to unique and fragile deep-sea ecosystems from the microscopic level to the ecosystem level. One goal is to improve understanding, at the microbiological scale, of the benthic communities (including corals) that reside in and around mid-Atlantic canyon habitats and their associated environments. Specific objectives include identifying and characterizing the microbial associates of deep-sea corals, characterizing the microbial biofilms on hard substrates to better determine their role in engineering the ecosystem, and adding a microbial dimension to benthic community structure and function assessments by characterizing micro-eukaryotes, bacteria, and archaea in deep-sea sediments.

Energy Savings Potential and RD&D Opportunities for Commercial Building HVAC Systems

DOE Office of Scientific and Technical Information (OSTI.GOV)

Goetzler, William; Shandross, Richard; Young, Jim

The Building Technologies Office (BTO) commissioned this characterization and technology assessment of heating, ventilation, and air-conditioning (HVAC) systems for commercial buildings. The main objectives of this study: Identify a wide range of technology options in varying stages of development that could reduce commercial HVAC energy consumption; Characterize these technology options based on their technical energy-savings potential, development status, non-energy benefits, and other factors affecting end-user acceptance and the ability to compete with conventional HVAC technologies; Make specific recommendations to DOE and other stakeholders on potential research, development, and demonstration (RD&D) activities that would support further development of the most promisingmore » technology options.« less

Patient Characterization Protocols for Psychophysiological Studies of Traumatic Brain Injury and Post-TBI Psychiatric Disorders

PubMed Central

Rapp, Paul E.; Rosenberg, Brenna M.; Keyser, David O.; Nathan, Dominic; Toruno, Kevin M.; Cellucci, Christopher J.; Albano, Alfonso M.; Wylie, Scott A.; Gibson, Douglas; Gilpin, Adele M. K.; Bashore, Theodore R.

2013-01-01

Psychophysiological investigations of traumatic brain injury (TBI) are being conducted for several reasons, including the objective of learning more about the underlying physiological mechanisms of the pathological processes that can be initiated by a head injury. Additional goals include the development of objective physiologically based measures that can be used to monitor the response to treatment and to identify minimally symptomatic individuals who are at risk of delayed-onset neuropsychiatric disorders following injury. Research programs studying TBI search for relationships between psychophysiological measures, particularly ERP (event-related potential) component properties (e.g., timing, amplitude, scalp distribution), and a participant’s clinical condition. Moreover, the complex relationships between brain injury and psychiatric disorders are receiving increased research attention, and ERP technologies are making contributions to this effort. This review has two objectives supporting such research efforts. The first is to review evidence indicating that TBI is a significant risk factor for post-injury neuropsychiatric disorders. The second objective is to introduce ERP researchers who are not familiar with neuropsychiatric assessment to the instruments that are available for characterizing TBI, post-concussion syndrome, and psychiatric disorders. Specific recommendations within this very large literature are made. We have proceeded on the assumption that, as is typically the case in an ERP laboratory, the investigators are not clinically qualified and that they will not have access to participant medical records. PMID:23885250

On Using Intensity Interferometry for Feature Identification and Imaging of Remote Objects

NASA Technical Reports Server (NTRS)

Erkmen, Baris I.; Strekalov, Dmitry V.; Yu, Nan

2013-01-01

We derive an approximation to the intensity covariance function of two scanning pinhole detectors, facing a distant source (e.g., a star) being occluded partially by an absorptive object (e.g., a planet). We focus on using this technique to identify or image an object that is in the line-of-sight between a well-characterized source and the detectors. We derive the observed perturbation to the intensity covariance map due to the object, showing that under some reasonable approximations it is proportional to the real part of the Fourier transform of the source's photon-flux density times the Fourier transform of the object's intensity absorption. We highlight the key parameters impacting its visibility and discuss the requirements for estimating object-related parameters, e.g., its size, velocity or shape. We consider an application of this result to determining the orbit inclination of an exoplanet orbiting a distant star. Finally, motivated by the intrinsically weak nature of the signature, we study its signal-to-noise ratio and determine the impact of system parameters.

Nurse-physician leadership: insights into interprofessional collaboration.

PubMed

Clark, Rebecca Culver; Greenawald, Mark

2013-12-01

The objective of this qualitative research study was to identify themes characterizing collaboration from the perspectives of nurses and physicians serving in complementary leadership roles in intensive and progressive care hospital units. Failures of communication are reported as a major cause of sentinel events. Most frequently, communication breakdown occurs between physicians and nurses. In this qualitative research study, taped interviews with nursing and medical unit directors (physicians) were analyzed for themes regarding factors influencing collaboration. Themes identified included the impact of organizational support, shared expectations, relationships, and communication. Findings of this study support the need for organizations and professionals to facilitate deliberate, structured interprofessional communication to advance collaboration between nurses and physicians.

Lessons for Interstellar Travel from the Guidance and Control Design of the Near Earth Asteroid Scout Solar Sail Mission

NASA Technical Reports Server (NTRS)

Diedrich, Benjamin; Heaton, Andrew

2017-01-01

NASA's Near Earth Asteroid Scout (NEA Scout) solar sail mission will fly by and image an asteroid. The team has experience characterizing the sail forces and torques used in guidance, navigation, and control to meet the scientific objectives. Interstellar and precursor sail missions similarly require understanding of beam riding dynamics to follow sufficiently accurate trajectories to perform their missions. Objective: Identify the driving factors required to implement a guidance and control system that meets mission requirements for a solar sail mission; Compare experience of an asteroid flyby mission to interstellar missions to flyby and observe other stars or precursor missions to study the extrasolar medium.

Children in poverty: resilience despite risk.

PubMed

Garmezy, N

1993-02-01

Two objectives provided the focus for the Conference on Community Violence and Children's Development that was jointly sponsored by the National Institute of Mental Health and the John D. and Catherine T. MacArthur Foundation. One was to examine the evidence for deficit behaviors that characterized children reared in poverty; the second was to identify the characteristics of children who sustained their competencies despite being reared in comparable environments. These dual objectives took this form: "What can we conclude from studies of children, their families, and environments about characteristics that predispose children to maladjustment following exposure to violence, and about characteristics that protect children from such adjustment problems following, or in the midst of, violence exposure?"

Orbital Debris Characterization via Laboratory Optical Measurements

NASA Technical Reports Server (NTRS)

Cowardin, Healther

2011-01-01

Optical observations of orbital debris offer insights that differ from radar measurements (specifically the size parameter,wavelength regime,and altitude range). For example, time-dependent photometric data yield lightcurves in multiple bandpasses that aid in material identification and possible periodic orientations. These data can also be used to help identify shapes and optical properties at multiple phase angles. Capitalizing on optical data products and applying them to generate a more complete understanding of orbital objects is a key objective of NASA's Optical Measurement Program, and the primary reason for the creation of the Optical Measurements Center(OMC). The OMC attempts to emulate space-based illumination conditions using equipment and techniques that parallel telescopic observations and source-target-sensor orientations.

Chemical characterization of atmospheric particles and source apportionment in the vicinity of a steelmaking industry.

PubMed

Almeida, S M; Lage, J; Fernández, B; Garcia, S; Reis, M A; Chaves, P C

2015-07-15

The objective of this work was to provide a chemical characterization of atmospheric particles collected in the vicinity of a steelmaking industry and to identify the sources that affect PM10 levels. A total of 94 PM samples were collected in two sampling campaigns that occurred in February and June/July of 2011. PM2.5 and PM2.5-10 were analyzed for a total of 22 elements by Instrumental Neutron Activation Analysis and Particle Induced X-ray Emission. The concentrations of water soluble ions in PM10 were measured by Ion Chromatography and Indophenol-Blue Spectrophotometry. Positive Matrix Factorization receptor model was used to identify sources of particulate matter and to determine their mass contribution to PM10. Seven main groups of sources were identified: marine aerosol identified by Na and Cl (22%), steelmaking and sinter plant represented by As, Cr, Cu, Fe, Ni, Mn, Pb, Sb and Zn (11%), sinter plant stack identified by NH4(+), K and Pb (12%), an unidentified Br source (1.8%), secondary aerosol from coke making and blast furnace (19%), fugitive emissions from the handling of raw material, sinter plant and vehicles dust resuspension identified by Al, Ca, La, Si, Ti and V (14%) and sinter plant and blast furnace associated essentially with Fe and Mn (21%). Copyright © 2015 Elsevier B.V. All rights reserved.

Transmutation studies at CEA in frame of the SPIN program objectives, results and future trends

DOE Office of Scientific and Technical Information (OSTI.GOV)

Salvatores, M.; Prunier, C.; Guerin, Y.

1995-10-01

In order to respond to the public concern about wastes and in particular the long-lived high level ones, a French law issued on December 30, 1991 identified the major objectives of research for the next fifteen years, before a new debate and possibly a decision on final wastes disposal in Parliament. These objectives are: (1) improvement of the wastes conditioning; (2) extraction and transmutation of the long-lived wastes in order to minimize their long term toxicity; (3) research performed in underground laboratories in order to characterize the capacity of geological structures to confine radioactive wastes (two sites have to bemore » selected for these underground laboratories, in concertation with the local population); (4) last, the study of conditioning and prolonged surface storage of wastes.« less

Towards a sustainable diet combining economic, environmental and nutritional objectives.

PubMed

Donati, Michele; Menozzi, Davide; Zighetti, Camilla; Rosi, Alice; Zinetti, Anna; Scazzina, Francesca

2016-11-01

Foods consumed and dietary patterns are strong determinants of health status. Diet and nutrition have a key role in health promotion and maintenance during the entire lifetime, but what we choose to eat and drink greatly affects the environmental impact on ecosystems as well as monetary resources. Some studies suggest that a healthy diet with a low environmental impact is not necessarily more expensive. This paper aims to identify a healthy, greener and cheaper diet based on current consumption patterns. Dietary information was collected from 104 young adults in the last year of high school in Parma (Italy). Diet was monitored with 7-day dietary records. Subsequently, food items were decoded to obtain nutritional, economic and environmental impact data. An optimization tool based on mathematical programming (Multi-Objective Linear Programming) was used to identify sustainable diet. Three different 7-day diets were identified, based on nutrition recommendations for the healthy Italian adult population, characterized by different targets and optimizing different impacts: first the diet at the lowest cost (Minimum Cost Diet - MCD), then the Environmentally Sustainable Diet (ESD) obtained by minimizing the three environmental indicators (CO2e emissions, H2O consumption and amount of land to regenerate the resources - m(2)). Finally, the Sustainable Diet (SD) was identified by integrating environmental and economic sustainability objectives. Lastly, suggestions and recommendations for communication campaigns and other interventions to achieve sustainable diet are suggested. Copyright © 2016 Elsevier Ltd. All rights reserved.

Early phases in the stellar and substellar formation and evolution. Infrared and submillimeter data in the Barnard 30 dark cloud

NASA Astrophysics Data System (ADS)

Barrado, D.; de Gregorio Monsalvo, I.; Huélamo, N.; Morales-Calderón, M.; Bayo, A.; Palau, A.; Ruiz, M. T.; Rivière-Marichalar, P.; Bouy, H.; Morata, Ó.; Stauffer, J. R.; Eiroa, C.; Noriega-Crespo, A.

2018-04-01

Aims: The early evolutionary stage of brown dwarfs (BDs) is not very well characterized, especially during the embedded phase. Our goal is to gain insight into the dominant formation mechanism of very low-mass objects and BDs. Methods: We have conducted deep observations at 870 μm obtained with the LABOCA bolometer at the APEX telescope in order to identify young submillimeter (submm) sources in the Barnard 30 dark cloud. We have complemented these data with multi-wavelength observations from the optical to the far-IR and compiled complete spectral energy distributions in order to identify the counterparts, characterize the sources and to assess their membership to the association and stellar or substellar status based on the available photometric information. Results: We have identified 34 submm sources and a substantial number of possible and probable Barnard 30 members within each individual APEX/LABOCA beam. They can be classified into three distinct groups. First, 15 of these 34 have a clear optical or IR counterpart to the submm peak and nine of them are potential proto-BD candidates. Moreover, a substantial number of them could be multiple systems. A second group of 13 sources comprises candidate members with significant infrared excesses located away from the central submm emission. All of them include BD candidates, some displaying IR excess, but their association with submm emission is unclear. In addition, we have found six starless cores and, based on the total dust mass estimate, three might be pre-substellar (or pre-BDs) cores. Finally, the complete characterization of our APEX/LABOCA sources, focusing on those detected at 24 and/or 70 μm, indicates that in our sample of 34 submm sources there are, at least: two WTTs, four CTTs, five young stellar objects, eight proto-BD candidates (with another three dubious cases), and one very low luminosity objects. Conclusions: Our findings provide additional evidence concerning the BD formation mechanism, which seems to be a downsized version of the stellar formation. Tables 3-7 and reduced images (FITS files) are only available at the CDS via anonymous ftp to http://cdsarc.u-strasbg.fr (http://130.79.128.5) or via http://cdsarc.u-strasbg.fr/viz-bin/qcat?J/A+A/vol/page

Science Goals, Objectives, and Investigations of the 2016 Europa Lander Science Definition Team Report

NASA Astrophysics Data System (ADS)

Hand, Kevin P.; Murray, Alison; Garvin, James; and the Europa Lander Science Definition Team, Project Science Team, and Project Engineering Team.

2017-10-01

In June of 2016 NASA convened a 21-person team of scientists to establish the science goals, objectives, investigations, measurement requirements, and model payload of a Europa lander mission concept. The NASA HQ Charter goals, in priority order, are as follows:1) Search for evidence of life on Europa, 2) Assess the habitability of Europa via in situ techniques uniquely available to a lander mission, 3) Characterize surface and subsurface properties at the scale of the lander to support future exploration of Europa.Within Goal 1, four Objectives were developed for seeking signs of life. These include the need to: a) detect and characterize any organic indicators of past or present life, b) identify and characterize morphological, textural, and other indicators of life, c) detect and characterize any inorganic indicators of past or present life, and d) determine the provenance of Lander-sampled material. Goal 2 focuses on Europa’s habitability and ensures that even in the absence of the detection of any potential biosignatures, significant ocean world science is still achieved. Goal 3 ensures that the landing site region is quantitatively characterized in the context needed for Goals 1 and 2, and that key measurements about Europa’s ice shell are made to enable future exploration.Critically, scientific success cannot be, and should never be, contingent on finding signs of life - such criteria would be levying requirements on how the universe works. Rather, scientific success is defined here as achieving a suite of measurements such that if convincing signs of life are present on Europa’s surface they could be detected at levels comparable to those found in benchmark environments on Earth, and, further, that even if no potential biosignatures are detected, the science return of the mission will significantly advance our fundamental understanding of Europa’s chemistry, geology, geophysics, and habitability.

Data Analysis Techniques for Ligo Detector Characterization

NASA Astrophysics Data System (ADS)

Valdes Sanchez, Guillermo A.

Gravitational-wave astronomy is a branch of astronomy which aims to use gravitational waves to collect observational data about astronomical objects and events such as black holes, neutron stars, supernovae, and processes including those of the early universe shortly after the Big Bang. Einstein first predicted gravitational waves in the early century XX, but it was not until Septem- ber 14, 2015, that the Laser Interferometer Gravitational-Wave Observatory (LIGO) directly ob- served the first gravitational waves in history. LIGO consists of two twin detectors, one in Livingston, Louisiana and another in Hanford, Washington. Instrumental and sporadic noises limit the sensitivity of the detectors. Scientists conduct Data Quality studies to distinguish a gravitational-wave signal from the noise, and new techniques are continuously developed to identify, mitigate, and veto unwanted noise. This work presents the application of data analysis techniques, such as Hilbert-Huang trans- form (HHT) and Kalman filtering (KF), in LIGO detector characterization. We investigated the application of HHT to characterize the gravitational-wave signal of the first detection, we also demonstrated the functionality of HHT identifying noise originated from light being scattered by perturbed surfaces, and we estimated thermo-optical aberration using KF. We put particular attention to the scattering origin application, for which a tool was developed to identify disturbed surfaces originating scattering noise. The results reduced considerably the time to search for the scattering surface and helped LIGO commissioners to mitigate the noise.

Study of Pure Proteins, Nucleic Acids and their Complexes from Extreme Halobacteria of the Dead Sea: RNA Polymerase-DNA Interaction

DTIC Science & Technology

1988-10-10

identify by block number) FIELD GROUP S OUP - Archaebacteria , Halobacteria, Proteins Nucleic Acids, 08 RNA Polymerase-DNA Interactionsi R soimal operons...objectives of our program are to isolate and characterize a fully active DNA dependent RNA polymerase from the extremely halophilic archaebacteria from...Woese and his colleagues to suggest that all living organisms can be classified into three phylogenetic kingdoms : the eukaryotes, the eubacterla and

Textural features for radar image analysis

NASA Technical Reports Server (NTRS)

Shanmugan, K. S.; Narayanan, V.; Frost, V. S.; Stiles, J. A.; Holtzman, J. C.

1981-01-01

Texture is seen as an important spatial feature useful for identifying objects or regions of interest in an image. While textural features have been widely used in analyzing a variety of photographic images, they have not been used in processing radar images. A procedure for extracting a set of textural features for characterizing small areas in radar images is presented, and it is shown that these features can be used in classifying segments of radar images corresponding to different geological formations.

Characterization of volatile compounds responsible for the aroma in naturally fermented sausages by gas chromatography-olfactometry.

PubMed

Olivares, Alicia; Navarro, José Luis; Flores, Mónica

2015-03-01

The objective of this study was to characterize naturally fermented dry sausages produced without the use of microbial starters and to determine which odour-active compounds are responsible for their aroma. The traditional manufacture was responsible for different chemical characteristics and consumer's acceptance. The volatile compounds detected in the headspace comprised a complex mixture of volatile compounds derived from bacterial metabolism (mainly esterase activity of Staphyloccoci), spices and lipid auto-oxidation. The odour-active volatile compounds were identified using gas chromatography coupled to olfactometry (GC-O) using the detection frequency method. The aroma profile was characterized by the presence of several compounds such as acetic acid, ethyl butanoate, hexanal, methional, 1-octen-3-ol, benzeneacetaldehyde and 4-methyl-phenol. However, naturally fermented sausages were also characterized by numerous esters, both ethyl and methyl esters, which impart a wide variety of fruity notes. © The Author(s) 2013 Reprints and permissions: sagepub.co.uk/journalsPermissions.nav.

Identification and characterization of the elusive mutation causing the historical von Willebrand Disease type IIC Miami.

PubMed

Obser, T; Ledford-Kraemer, M; Oyen, F; Brehm, M A; Denis, C V; Marschalek, R; Montgomery, R R; Sadler, J E; Schneppenheim, S; Budde, U; Schneppenheim, R

2016-09-01

Essentials Von Willebrand disease IIC Miami features high von Willebrand factor (VWF) with reduced function. We aimed to identify and characterize the elusive underlying mutation in the original family. An inframe duplication of VWF exons 9-10 was identified and characterized. The mutation causes a defect in VWF multimerization and decreased VWF clearance from the circulation. Background A variant of von Willebrand disease (VWD) type 2A, phenotype IIC (VWD2AIIC), is characterized by recessive inheritance, low von Willebrand factor antigen (VWF:Ag), lack of VWF high-molecular-weight multimers, absence of VWF proteolytic fragments and mutations in the VWF propeptide. A family with dominantly inherited VWD2AIIC but markedly elevated VWF:Ag of > 2 U L(-1) was described as VWD type IIC Miami (VWD2AIIC-Miami) in 1993; however, the molecular defect remained elusive. Objectives To identify the molecular mechanism underlying the phenotype of the original VWD2AIIC-Miami. Patients and Methods We studied the original family with VWD2AIIC-Miami phenotypically and by genotyping. The identified mutation was recombinantly expressed and characterized by standard techniques, confocal imaging and in a mouse model, respectively. Results By Multiplex ligation-dependent probe amplification we identified an in-frame duplication of VWF exons 9-10 (c.998_1156dup; p.Glu333_385dup) in all patients. Recombinant mutant (rm)VWF only presented as a dimer. Co-expressed with wild-type VWF, the multimer pattern was indistinguishable from patients' plasma VWF. Immunofluorescence studies indicated retention of rmVWF in unusually large intracellular granules in the endoplasmic reticulum. ADAMTS-13 proteolysis of rmVWF under denaturing conditions was normal; however, an aberrant proteolytic fragment was apparent. A decreased ratio of VWF propeptide to VWF:Ag and a 1-desamino-8-d-arginine vasopressin (DDAVP) test in one patient indicated delayed VWF clearance, which was supported by clearance data after infusion of rmVWF into VWF(-/-) mice. Conclusion The unique phenotype of VWD2 type IIC-Miami results from dominant impairment of multimer assembly, an aberrant structure of mutant mature VWF and reduced clearance in vivo. © 2016 International Society on Thrombosis and Haemostasis.

Characterization of the canine urinary proteome.

PubMed

Brandt, Laura E; Ehrhart, E J; Scherman, Hataichanok; Olver, Christine S; Bohn, Andrea A; Prenni, Jessica E

2014-06-01

Urine is an attractive biofluid for biomarker discovery as it is easy and minimally invasive to obtain. While numerous studies have focused on the characterization of human urine, much less research has focused on canine urine. The objectives of this study were to characterize the universal canine urinary proteome (both soluble and exosomal), to determine the overlap between the canine proteome and a representative human urinary proteome study, to generate a resource for future canine studies, and to determine the suitability of the dog as a large animal model for human diseases. The soluble and exosomal fractions of normal canine urine were characterized using liquid chromatography tandem mass spectrometry (LC-MS/MS). Biological Networks Gene Ontology (BiNGO) software was utilized to assign the canine urinary proteome to respective Gene Ontology categories, such as Cellular Component, Molecular Function, and Biological Process. Over 500 proteins were confidently identified in normal canine urine. Gene Ontology analysis revealed that exosomal proteins were largely derived from an intracellular location, while soluble proteins included both extracellular and membrane proteins. Exosome proteins were assigned to metabolic processes and localization, while soluble proteins were primarily annotated to specific localization processes. Several proteins identified in normal canine urine have previously been identified in human urine where these proteins are related to various extrarenal and renal diseases. The results of this study illustrate the potential of the dog as an animal model for human disease states and provide the framework for future studies of canine renal diseases. © 2014 American Society for Veterinary Clinical Pathology and European Society for Veterinary Clinical Pathology.

Characterization of lymphocyte populations in nonspecific interstitial pneumonia*

PubMed Central

Keogh, Karina A; Limper, Andrew H

2005-01-01

Study objectives Nonspecific interstitial pneumonia (NSIP) has been identified as a distinct entity with a more favorable prognosis and better response to immunosuppressive therapies than usual interstitial pneumonia (UIP). However the inflammatory profile of NSIP has not been characterized. Design Using immunohistochemistry techniques on open lung biopsy specimens, the infiltrate in NSIP was characterized in terms of T and B cells, and macrophages, and the T cell population further identified as either CD4 (helper) or CD8 (suppressor-cytotoxic) T cells. The extent of Th1 and Th2 cytokine producing cells was determined and compared to specimens from patients with UIP. Results In ten NSIP tissue samples 41.4 ± 4% of mononuclear cells expressed CD3, 24.7 ± 1.8% CD4, 19.1 ± 2% CD8, 27.4 ± 3.9% CD20, and 14.3 ± 1.6% had CD68 expression. Mononuclear cells expressed INFγ 21.9 ± 1.9% of the time and IL-4 in 3.0 ± 1%. In contrast, biopsies from eight patients with UIP demonstrated substantially less cellular staining for either cytokine (INFγ; 4.6 ± 1.7% and IL-4; 0.6 ± 0.3%). Significant populations of CD20 positive B-cells were also identified. Conclusion The lymphocytic infiltrate in NSIP is characterized by an elevated CD4/CD8 T-cell ratio, and is predominantly of Th1 type, with additional populations rich in B-cells. Such features are consistent with the favorable clinical course observed in patients with NSIP compared to UIP. PMID:16287509

SNP Discovery by Illumina-Based Transcriptome Sequencing of the Olive and the Genetic Characterization of Turkish Olive Genotypes Revealed by AFLP, SSR and SNP Markers

PubMed Central

Kaya, Hilal Betul; Cetin, Oznur; Kaya, Hulya; Sahin, Mustafa; Sefer, Filiz; Kahraman, Abdullah; Tanyolac, Bahattin

2013-01-01

Background The olive tree (Olea europaea L.) is a diploid (2n = 2x = 46) outcrossing species mainly grown in the Mediterranean area, where it is the most important oil-producing crop. Because of its economic, cultural and ecological importance, various DNA markers have been used in the olive to characterize and elucidate homonyms, synonyms and unknown accessions. However, a comprehensive characterization and a full sequence of its transcriptome are unavailable, leading to the importance of an efficient large-scale single nucleotide polymorphism (SNP) discovery in olive. The objectives of this study were (1) to discover olive SNPs using next-generation sequencing and to identify SNP primers for cultivar identification and (2) to characterize 96 olive genotypes originating from different regions of Turkey. Methodology/Principal Findings Next-generation sequencing technology was used with five distinct olive genotypes and generated cDNA, producing 126,542,413 reads using an Illumina Genome Analyzer IIx. Following quality and size trimming, the high-quality reads were assembled into 22,052 contigs with an average length of 1,321 bases and 45 singletons. The SNPs were filtered and 2,987 high-quality putative SNP primers were identified. The assembled sequences and singletons were subjected to BLAST similarity searches and annotated with a Gene Ontology identifier. To identify the 96 olive genotypes, these SNP primers were applied to the genotypes in combination with amplified fragment length polymorphism (AFLP) and simple sequence repeats (SSR) markers. Conclusions/Significance This study marks the highest number of SNP markers discovered to date from olive genotypes using transcriptome sequencing. The developed SNP markers will provide a useful source for molecular genetic studies, such as genetic diversity and characterization, high density quantitative trait locus (QTL) analysis, association mapping and map-based gene cloning in the olive. High levels of genetic variation among Turkish olive genotypes revealed by SNPs, AFLPs and SSRs allowed us to characterize the Turkish olive genotype. PMID:24058483

Objective sampling design in a highly heterogeneous landscape - characterizing environmental determinants of malaria vector distribution in French Guiana, in the Amazonian region.

PubMed

Roux, Emmanuel; Gaborit, Pascal; Romaña, Christine A; Girod, Romain; Dessay, Nadine; Dusfour, Isabelle

2013-12-01

Sampling design is a key issue when establishing species inventories and characterizing habitats within highly heterogeneous landscapes. Sampling efforts in such environments may be constrained and many field studies only rely on subjective and/or qualitative approaches to design collection strategy. The region of Cacao, in French Guiana, provides an excellent study site to understand the presence and abundance of Anopheles mosquitoes, their species dynamics and the transmission risk of malaria across various environments. We propose an objective methodology to define a stratified sampling design. Following thorough environmental characterization, a factorial analysis of mixed groups allows the data to be reduced and non-collinear principal components to be identified while balancing the influences of the different environmental factors. Such components defined new variables which could then be used in a robust k-means clustering procedure. Then, we identified five clusters that corresponded to our sampling strata and selected sampling sites in each stratum. We validated our method by comparing the species overlap of entomological collections from selected sites and the environmental similarities of the same sites. The Morisita index was significantly correlated (Pearson linear correlation) with environmental similarity based on i) the balanced environmental variable groups considered jointly (p = 0.001) and ii) land cover/use (p-value < 0.001). The Jaccard index was significantly correlated with land cover/use-based environmental similarity (p-value = 0.001). The results validate our sampling approach. Land cover/use maps (based on high spatial resolution satellite images) were shown to be particularly useful when studying the presence, density and diversity of Anopheles mosquitoes at local scales and in very heterogeneous landscapes.

Objective sampling design in a highly heterogeneous landscape - characterizing environmental determinants of malaria vector distribution in French Guiana, in the Amazonian region

PubMed Central

2013-01-01

Background Sampling design is a key issue when establishing species inventories and characterizing habitats within highly heterogeneous landscapes. Sampling efforts in such environments may be constrained and many field studies only rely on subjective and/or qualitative approaches to design collection strategy. The region of Cacao, in French Guiana, provides an excellent study site to understand the presence and abundance of Anopheles mosquitoes, their species dynamics and the transmission risk of malaria across various environments. We propose an objective methodology to define a stratified sampling design. Following thorough environmental characterization, a factorial analysis of mixed groups allows the data to be reduced and non-collinear principal components to be identified while balancing the influences of the different environmental factors. Such components defined new variables which could then be used in a robust k-means clustering procedure. Then, we identified five clusters that corresponded to our sampling strata and selected sampling sites in each stratum. Results We validated our method by comparing the species overlap of entomological collections from selected sites and the environmental similarities of the same sites. The Morisita index was significantly correlated (Pearson linear correlation) with environmental similarity based on i) the balanced environmental variable groups considered jointly (p = 0.001) and ii) land cover/use (p-value << 0.001). The Jaccard index was significantly correlated with land cover/use-based environmental similarity (p-value = 0.001). Conclusions The results validate our sampling approach. Land cover/use maps (based on high spatial resolution satellite images) were shown to be particularly useful when studying the presence, density and diversity of Anopheles mosquitoes at local scales and in very heterogeneous landscapes. PMID:24289184

Chemical characterization and evaluation of antioxidant properties of açaí fruits (Euterpe oleraceae Mart.) during ripening.

PubMed

Gordon, André; Cruz, Ana Paula Gil; Cabral, Lourdes Maria Corrêa; de Freitas, Sidinéa Cordeiro; Taxi, Cristina Maria Araujo Dib; Donangelo, Carmen Marino; de Andrade Mattietto, Rafaella; Friedrich, Mirko; da Matta, Virgínia Martins; Marx, Friedhelm

2012-07-15

Consumption of açaí fruits has been linked to positive health effects due to its phenolic content and nutritive value. The objective of this study was to characterize açaí fruits chemically and to determine the antioxidant capacity at three different maturity stages. With the exception of fat, amounts of macronutrients, minerals and titratable acids decreased during the ripening process. The same trend was observed for most of the phenolic constituents identified by HPLC-ESI-MS/MS. A consistent decline was shown for flavones and hydroxycinnamic acids. The concentration of the anthocyanins increased in the course of ripening. In accordance with the total amount of the identified phenolic compounds, the antioxidant capacity, measured by TEAC and TOSC, also decreased. However, the contribution of the main phenolic compounds to the overall antioxidant capacity evaluated by TOSC was estimated to be low. Copyright © 2012. Published by Elsevier Ltd.

Characterization of Cleaning and Disinfecting Tasks and Product Use Among Hospital Occupations

PubMed Central

Saito, Rena; Virji, M. Abbas; Henneberger, Paul K.; Humann, Michael J.; LeBouf, Ryan F.; Stanton, Marcia L.; Liang, Xiaoming; Stefaniak, Aleksandr B.

2016-01-01

Background Healthcare workers have an elevated prevalence of asthma and related symptoms associated with the use of cleaning/disinfecting products. The objective of this study was to identify and characterize cleaning/disinfecting tasks and products used among hospital occupations. Methods Workers from 14 occupations at five hospitals were monitored for 216 shifts, and work tasks and products used were recorded at five-minute intervals. The major chemical constituents of each product were identified from safety data sheets. Results Cleaning and disinfecting tasks were performed with a high frequency at least once per shift in many occupations. Medical equipment preparers, housekeepers, floor strippers/waxers, and endoscopy technicians spent on average 108–177 min/shift performing cleaning/disinfecting tasks. Many occupations used products containing amines and quaternary ammonium compounds for > 100 min/shift. Conclusions This analysis demonstrates that many occupations besides housekeeping incur exposures to cleaning/disinfecting products, albeit for different durations and using products containing different chemicals. PMID:25351791

The Mission Accessible Near-Earth Objects Survey (MANOS): First Photometric Results

NASA Astrophysics Data System (ADS)

Thirouin, A.; Moskovitz, N.; Binzel, R. P.; Christensen, E.; DeMeo, F. E.; Person, M. J.; Polishook, D.; Thomas, C. A.; Trilling, D.; Willman, M.; Hinkle, M.; Burt, B.; Avner, D.; Aceituno, F. J.

2016-12-01

The Mission Accessible Near-Earth Objects Survey aims to physically characterize sub-km near-Earth objects (NEOs). We report the first photometric results from the survey that began in 2013 August. Photometric observations were performed using 1-4 m class telescopes around the world. We present rotational periods and light curve amplitudes for 86 sub-km NEOs, though in some cases only lower limits are provided. Our main goal is to obtain light curves for small NEOs (typically, sub-km objects) and estimate their rotational periods, light curve amplitudes, and shapes. These properties are used for a statistical study to constrain overall properties of the NEO population. A weak correlation seems to indicate that smaller objects are more spherical than larger ones. We also report seven NEOs that are fully characterized (light curve and visible spectra) as the most suitable candidates for a future human or robotic mission. Viable mission targets are objects fully characterized, with Δv NHATS ≤ 12 km s-1, and a rotational period P > 1 hr. Assuming a similar rate of object characterization as reported in this paper, approximately 1230 NEOs need to be characterized in order to find 100 viable mission targets.

Serendipitous discovery of quadruply imaged quasars: two diamonds

NASA Astrophysics Data System (ADS)

Lucey, John R.; Schechter, Paul L.; Smith, Russell J.; Anguita, T.

2018-05-01

Gravitationally lensed quasars are powerful and versatile astrophysical tools, but they are challengingly rare. In particular, only ˜25 well-characterized quadruple systems are known to date. To refine the target catalogue for the forthcoming Taipan Galaxy Survey, the images of a large number of sources are being visually inspected in order to identify objects that are confused by a foreground star or galaxies that have a distinct multicomponent structure. An unexpected by-product of this work has been the serendipitous discovery of about a dozen galaxies that appear to be lensing quasars, i.e. pairs or quartets of foreground stellar objects in close proximity to the target source. Here, we report two diamond-shaped systems. Follow-up spectroscopy with the IMACS instrument on the 6.5m Magellan Baade telescope confirms one of these as a z = 1.975 quasar quadruply lensed by a double galaxy at z = 0.293. Photometry from publicly available survey images supports the conclusion that the other system is a highly sheared quadruply imaged quasar. In starting with objects thought to be galaxies, our lens finding technique complements the conventional approach of first identifying sources with quasar-like colours and subsequently finding evidence of lensing.

Fluid-Rock Characterization and Interactions in NMR Well Logging

DOE Office of Scientific and Technical Information (OSTI.GOV)

George J. Hirasaki; Kishore K. Mohanty

2005-09-05

The objective of this report is to characterize the fluid properties and fluid-rock interactions that are needed for formation evaluation by NMR well logging. The advances made in the understanding of NMR fluid properties are summarized in a chapter written for an AAPG book on NMR well logging. This includes live oils, viscous oils, natural gas mixtures, and the relation between relaxation time and diffusivity. Oil based drilling fluids can have an adverse effect on NMR well logging if it alters the wettability of the formation. The effect of various surfactants on wettability and surface relaxivity are evaluated for silicamore » sand. The relation between the relaxation time and diffusivity distinguishes the response of brine, oil, and gas in a NMR well log. A new NMR pulse sequence in the presence of a field gradient and a new inversion technique enables the T{sub 2} and diffusivity distributions to be displayed as a two-dimensional map. The objectives of pore morphology and rock characterization are to identify vug connectivity by using X-ray CT scan, and to improve NMR permeability correlation. Improved estimation of permeability from NMR response is possible by using estimated tortuosity as a parameter to interpolate between two existing permeability models.« less

Near-Earth Asteroid Returned Sample (NEARS)

NASA Technical Reports Server (NTRS)

Shoemaker, Eugene M.; Cheng, Andrew F.

1994-01-01

The concept of the Near-Earth Asteroid Returned Sample (NEARS) mission is to return to Earth 10-100 g from each of four to six sites on a near-Earth asteroid and to perform global characterization of the asteroid and measure mass, volume, and density to ten percent. The target asteroid for the mission is 4660 Nereus, probably a primitive C-type asteroid, with the alternate target being 1989ML, an extremely accessible asteroid of unknown type. Launch dates will be 1998, 2000, 2002, and 2004 on the Delta II-7925 launch vehicle. The mission objectives are three-fold. (1) Provide first direct and detailed petrological, chemical, age, and isotopic characterization of a near-Earth asteroid and relate it to terrestrial, lunar, and meteoritic materials. (2) Sample the asteroid regolith and characterize any exotic fragments. (3) Identify heterogeneity in the asteroid's isotopic properties, age, and elemental chemistry.

Functional analysis of chloroplast early light inducible proteins (ELIPs)

DOE Office of Scientific and Technical Information (OSTI.GOV)

Wetzel, Carolyn M

The objectives of this project were to characterize gene expression patterns of early light inducible protein (ELIP) genes in Arabidopsis thaliana and in Lycopersicon esculentum, to identify knock mutants of the 2 ELIP genes in Arabidopsis, and to characterize the effects of the knockouts. Expression in Arabidopsis was studied in response to thylakoid electron transport chain (PETC) capacity, where it was found that there is a signal for expression associated with reduction of the PETC. Expression in response to salt was also studied, with different responses of the two gene copies. Knockout lines for ELIP1 and ELIP2 have been identifiedmore » and are being characterized. In tomato, it was found that the single-copy ELIP gene is highly expressed in ripening fruit during the chloroplast-to-chromoplast transition. Studies of expression in tomato ripening mutants are ongoing.« less

Subthreshold depression and subjective cognitive complaints in Parkinson's disease.

PubMed

Santangelo, G; Vitale, C; Trojano, L; Angrisano, M G; Picillo, M; Errico, D; Agosti, V; Grossi, D; Barone, P

2014-03-01

Subthreshold depression (SubD) is characterized by clinically relevant depressive symptoms not meeting criteria for major depression. The possible association of SubD with subjective cognitive complaints and/or objective cognitive impairments was investigated in a sample of consecutive, non-demented Parkinson's disease (PD) outpatients. Amongst 115 patients, SubD was identified in 30 patients, major depression in 33; 36 patients were classified as non-depressed. Enrolled patients were administered tests and questionnaires validated in PD for assessing objective and subjective cognitive dysfunctions. On objective cognitive measures SubD patients did not differ from non-depressed patients, whereas depressed patients achieved significantly lower scores than the other two groups. SubD and depressed patients reported more cognitive complaints than non-depressed patients. SubD is a non-motor aspect of PD that is not related to objective cognitive deficits but is associated with subjective cognitive complaints, thus impacting on patients' well-being. © 2013 The Author(s) European Journal of Neurology © 2013 EFNS.

Tank 241-AY-101 Privatization Push Mode Core Sampling and Analysis Plan

DOE Office of Scientific and Technical Information (OSTI.GOV)

TEMPLETON, A.M.

2000-01-12

This sampling and analysis plan (SAP) identifies characterization objectives pertaining to sample collection, laboratory analytical evaluation, and reporting requirements for samples obtained from tank 241-AY-101. The purpose of this sampling event is to obtain information about the characteristics of the contents of 241-AY-101 required to satisfy Data Quality Objectives For RPP Privatization Phase I: Confirm Tank T Is An Appropriate Feed Source For High-Level Waste Feed Batch X(HLW DQO) (Nguyen 1999a), Data Quality Objectives For TWRS Privatization Phase I : Confirm Tank T Is An Appropriate Feed Source For Low-Activity Waste Feed Batch X (LAW DQO) (Nguyen 1999b), Low Activitymore » Waste and High-Level Waste Feed Data Quality Objectives (L and H DQO) (Patello et al. 1999), and Characterization Data Needs for Development, Design, and Operation of Retrieval Equipment Developed through the Data Quality Objective Process (Equipment DQO) (Bloom 1996). Special instructions regarding support to the LAW and HLW DQOs are provided by Baldwin (1999). Push mode core samples will be obtained from risers 15G and 150 to provide sufficient material for the chemical analyses and tests required to satisfy these data quality objectives. The 222-S Laboratory will extrude core samples; composite the liquids and solids; perform chemical analyses on composite and segment samples; archive half-segment samples; and provide subsamples to the Process Chemistry Laboratory. The Process Chemistry Laboratory will prepare test plans and perform process tests to evaluate the behavior of the 241-AY-101 waste undergoing the retrieval and treatment scenarios defined in the applicable DQOs. Requirements for analyses of samples originating in the process tests will be documented in the corresponding test plans and are not within the scope of this SAP.« less

Tank 241-AY-101 Privatization Push Mode Core Sampling and Analysis Plan

DOE Office of Scientific and Technical Information (OSTI.GOV)

TEMPLETON, A.M.

2000-05-19

This sampling and analysis plan (SAP) identifies characterization objectives pertaining to sample collection, laboratory analytical evaluation, and reporting requirements for samples obtained from tank 241-AY-101. The purpose of this sampling event is to obtain information about the characteristics of the contents of 241-AY-101 required to satisfy ''Data Quality Objectives For RPP Privatization Phase I: Confirm Tank T Is An Appropriate Feed Source For High-Level Waste Feed Batch X(HLW DQO)' (Nguyen 1999a), ''Data Quality Objectives For TWRS Privatization Phase I: Confirm Tank T Is An Appropriate Feed Source For Low-Activity Waste Feed Butch X (LAW DQO) (Nguyen 1999b)'', ''Low Activity Wastemore » and High-Level Waste Feed Data Quality Objectives (L&H DQO)'' (Patello et al. 1999), and ''Characterization Data Needs for Development, Design, and Operation of Retrieval Equipment Developed through the Data Quality Objective Process (Equipment DQO)'' (Bloom 1996). Special instructions regarding support to the LAW and HLW DQOs are provided by Baldwin (1999). Push mode core samples will be obtained from risers 15G and 150 to provide sufficient material for the chemical analyses and tests required to satisfy these data quality objectives. The 222-S Laboratory will extrude core samples; composite the liquids and solids; perform chemical analyses on composite and segment samples; archive half-segment samples; and provide sub-samples to the Process Chemistry Laboratory. The Process Chemistry Laboratory will prepare test plans and perform process tests to evaluate the behavior of the 241-AY-101 waste undergoing the retrieval and treatment scenarios defined in the applicable DQOs. Requirements for analyses of samples originating in the process tests will be documented in the corresponding test plans and are not within the scope of this SAP.« less

Advanced Platform Systems Technology study. Volume 2: Trade study and technology selection

NASA Technical Reports Server (NTRS)

1983-01-01

Three primary tasks were identified which include task 1-trade studies, task 2-trade study comparison and technology selection, and task 3-technology definition. Task 1 general objectives were to identify candidate technology trade areas, determine which areas have the highest potential payoff, define specific trades within the high payoff areas, and perform the trade studies. In order to satisfy these objectives, a structured, organized approach was employed. Candidate technology areas and specific trades were screened using consistent selection criteria and considering possible interrelationships. A data base comprising both manned and unmanned space platform documentation was used as a source of system and subsystem requirements. When requirements were not stated in the data base documentation, assumptions were made and recorded where necessary to characterize a particular spacecraft system. The requirements and assumptions were used together with the selection criteria to establish technology advancement goals and select trade studies. While both manned and unmanned platform data were used, the study was focused on the concept of an early manned space station.

Critical differences between elective and emergency surgery: identifying domains for quality improvement in emergency general surgery.

PubMed

Columbus, Alexandra B; Morris, Megan A; Lilley, Elizabeth J; Harlow, Alyssa F; Haider, Adil H; Salim, Ali; Havens, Joaquim M

2018-04-01

The objective of our study was to characterize providers' impressions of factors contributing to disproportionate rates of morbidity and mortality in emergency general surgery to identify targets for care quality improvement. Emergency general surgery is characterized by a high-cost burden and disproportionate morbidity and mortality. Factors contributing to these observed disparities are not comprehensively understood and targets for quality improvement have not been formally developed. Using a grounded theory approach, emergency general surgery providers were recruited through purposive-criterion-based sampling to participate in semi-structured interviews and focus groups. Participants were asked to identify contributors to emergency general surgery outcomes, to define effective care for EGS patients, and to describe operating room team structure. Interviews were performed to thematic saturation. Transcripts were iteratively coded and analyzed within and across cases to identify emergent themes. Member checking was performed to establish credibility of the findings. A total of 40 participants from 5 academic hospitals participated in either individual interviews (n = 25 [9 anesthesia, 12 surgery, 4 nursing]) or focus groups (n = 2 [15 nursing]). Emergency general surgery was characterized by an exceptionally high level of variability, which can be subcategorized as patient-variability (acute physiology and comorbidities) and system-variability (operating room resources and workforce). Multidisciplinary communication is identified as a modifier to variability in emergency general surgery; however, nursing is often left out of early communication exchanges. Critical variability in emergency general surgery may impact outcomes. Patient-variability and system-variability, with focus on multidisciplinary communication, represent potential domains for quality improvement in this field. Copyright © 2017 Elsevier Inc. All rights reserved.

Psychosocial profiles of people with pain associated with spinal cord injury: identification and comparison with other chronic pain syndromes.

PubMed

Widerström-Noga, Eva G; Duncan, Robert; Turk, Dennis C

2004-01-01

The objectives of the study were to: (1) identify psychosocial profiles of people with pain associated with spinal cord injuries (SCI), and (2) compare the psychosocial profiles with other chronic pain syndromes. History questionnaires and the Multidimensional Pain Inventory (MPI) were mailed to 159 persons with chronic pain and SCIs (SCICP) and 120 (75.5%) agreed to participate. Cluster analysis of MPI scores identified 2 subgroups of SCICP. One (42.5%) was characterized by high Pain Severity (PS), Affective Distress (AD), Life Interference (LI), and low Life Control (LC) similar to a previously identified cluster labeled Dysfunctional. The second (57.5%) was characterized by low PS, AD, LI, and high levels of LC and activity (GA), similar to the Adaptive Copers (AC) cluster identified in previous studies with the exception that the scores were lower on Support (S), Solicitous Responses (SR), and Negative Responses (NR) and higher on Distracting Responses (DR). Both subgroups were similar to those observed in other chronic pain syndromes (CPSs). People with SCIs differed significantly from heterogeneous chronic pain and chronic headaches in that they indicated less inference of pain with activities. They also scored lower on S. SCICP reported comparable levels of LI, LC, AD, S, SR, but greater levels of PS compared with a group of people with post-polio syndrome. The similarities and differences between SCI and other CPSs suggest that although a general measure assessing psychosocial impact can be used across pain syndromes, it is important to use different norms for comparison of particular pain syndromes. The failure to identify a subgroup of patients characterized by low levels of S and high levels of NR, and the low SR and DR for SCICP compared with other CPSs warrants examination.

A Characterization of Student Reflections in an Introductory Pharmacy Practice Experience Discussion Course.

PubMed

Dinkins, Melissa M; Haltom, Wesley R

2018-04-01

Objective. To characterize weekly student reflections in an introductory pharmacy practice experience (IPPE) discussion course meeting concurrently with IPPE rotations in institutional pharmacy. Methods. A qualitative analysis was conducted to identify themes within weekly reflective statements submitted by second year pharmacy students (P2) enrolled in an IPPE rotation and concurrent discussion course. Weekly reflections from the 2015-2016 offering of the course were reviewed by investigators to identify common themes via an iterative process. Subsequently, investigators coded each submission into one of the identified categories. Initial agreement between investigators was assessed using the Cohen kappa coefficient. Discrepancies between coding were resolved through discussion to reach consensus. Results. A total of 402 reflection assignments were reviewed from 85 P2 students enrolled in the IPPE course. Ten themes were identified, with the most common themes being interprofessional teamwork, pharmacist and technician roles and responsibilities, and policies and procedures. Substantial initial agreement between investigators was found, with the most discrepancies arising within the themes of medication distribution and pharmacy administration/organizational structure. Conclusion. Student reflections on IPPEs centered on 10 key topics, primarily related to distributive, legal, and regulatory functions of institutional pharmacy practice. Structuring an IPPE rotation longitudinally in an academic term, with a concurrent discussion course, builds a framework for regular student reflection.

Results from an experiment that collected visible-light polarization data using unresolved imagery for classification of geosynchronous satellites

NASA Astrophysics Data System (ADS)

Speicher, Andy; Matin, Mohammad; Tippets, Roger; Chun, Francis; Strong, David

2015-05-01

In order to protect critical military and commercial space assets, the United States Space Surveillance Network must have the ability to positively identify and characterize all space objects. Unfortunately, positive identification and characterization of space objects is a manual and labor intensive process today since even large telescopes cannot provide resolved images of most space objects. The objective of this study was to collect and analyze visible-spectrum polarization data from unresolved images of geosynchronous satellites taken over various solar phase angles. Different collection geometries were used to evaluate the polarization contribution of solar arrays, thermal control materials, antennas, and the satellite bus as the solar phase angle changed. Since materials on space objects age due to the space environment, their polarization signature may change enough to allow discrimination of identical satellites launched at different times. Preliminary data suggests this optical signature may lead to positive identification or classification of each satellite by an automated process on a shorter timeline. The instrumentation used in this experiment was a United States Air Force Academy (USAFA) Department of Physics system that consists of a 20-inch Ritchey-Chrétien telescope and a dual focal plane optical train fed with a polarizing beam splitter. Following a rigorous calibration, polarization data was collected during two nights on eight geosynchronous satellites built by various manufacturers and launched several years apart. When Stokes parameters were plotted against time and solar phase angle, the data indicates that a polarization signature from unresolved images may have promise in classifying specific satellites.

Application of LC-MS to the analysis of dyes in objects of historical interest

NASA Astrophysics Data System (ADS)

Zhang, Xian; Laursen, Richard

2009-07-01

High-performance liquid chromatography (HPLC) with photodiode array and mass spectrometric detection permits dyes extracted from objects of historical interest or from natural plant or animal dyestuffs to be characterized on the basis of three orthogonal properties: HPLC retention time, UV-visible spectrum and molecular mass. In the present study, we have focused primarily on yellow dyes, the bulk of which are flavonoid glycosides that would be almost impossible to characterize without mass spectrometric detection. Also critical for this analysis is a method for mild extraction of the dyes from objects (e.g., textiles) without hydrolyzing the glycosidic linkages. This was accomplished using 5% formic acid in methanol, rather than the more traditional 6 M HCl. Mass spectroscopy, besides providing the molecular mass of the dye molecule, sometimes yields additional structural data based on fragmentation patterns. In addition, coeluting compounds can often be detected using extracted ion chromatography. The utility of mass spectrometry is illustrated by the analysis of historical specimens of silk that had been dyed yellow with flavonoid glycosides from Sophora japonica (pagoda tree) and curcumins from Curcuma longa (turmeric). In addition, we have used these techniques to identify the dye type, and sometimes the specific dyestuff, in a variety of objects, including a yellow varnish from a 19th century Tibetan altar and a 3000-year-old wool mortuary textiles, from Xinjiang, China. We are using HPLC with diode array and mass spectrometric detection to create a library of analyzed dyestuffs (>200 so far; mostly plants) to serve as references for identification of dyes in objects of historical interest.

Surface density of quasars in two high-latitude fields

NASA Technical Reports Server (NTRS)

Usher, P. D.; Green, R. F.; Huang, K. L.; Warnock, A., III

1983-01-01

Fourty-four objects selected for ultraviolet excess have been identified spectroscopically. The objects lie in two Palomar 1.2 m Schmidt fields in the north galactic polar cap, one of 7.7 sq deg centered on Kapteyn Selected Area 29, the other of 36 sq deg centered on SA 55. The objects are characterized by Color Classes (CC) 1A, 1, 1B, 1C, 2, and 3. Quasars comprise 75 percent of the CC 1A objects and 44 percent of the objects in the SA 29 field. Twelve quasars in the SA 29 field comprise a complete sample to B = 18.5 mag, and given an uncorrected surface density of 1.6 quasars/sq deg. This value is essentially that derived by Sandage (1969). Corrections are applied to account for the lack of high redshift quasars. An empirical correction is derived to account for lack of simultaneity in selection and photometry. A corrected lower limit to the surface density is estimated to be 1.85 quasars/sq deg to B = 18.5 mag.

Integrating different perspectives on socialization theory and research: a domain-specific approach.

PubMed

Grusec, Joan E; Davidov, Maayan

2010-01-01

There are several different theoretical and research approaches to the study of socialization, characterized by frequently competing basic tenets and apparently contradictory evidence. As a way of integrating approaches and understanding discrepancies, it is proposed that socialization processes be viewed from a domain perspective, with each domain characterized by a particular form of social interaction between the object and agent of socialization and by specific socialization mechanisms and outcomes. It is argued that this approach requires researchers to identify the domain of social interaction they are investigating, to understand that phenotypically similar behaviors may belong to different domains, and to acknowledge that caregivers who are effective in one type of interaction may not be effective in another.

Spectroscopic Data for Characterizing the Atmospheres of Exoplanetsand Assigning Astronomical Spectra

NASA Astrophysics Data System (ADS)

Lee, Timothy J.

2018-06-01

In this talk I will discuss laboratory and computational efforts to provide detailed line list data for use in characterizing the atmospheres of planets, exoplanets, and other astrophysical objects such as dwarf stars. The discussion will cover significant efforts on stable molecules routinely found in atmospheres such as CO2, NH3, H2O, and SO2. In addition, there will be some discussion towards efforts to provide more limited line lists or simulated spectra for molecules that might be present in trace amounts, but would be very significant if identified, such as possible biosignatures. How these efforts may provide insight into astronomical observations, especially with the upcoming James Webb Space Telescope, will also be discussed.

Extremely red quasars from SDSS, BOSS and WISE: classification of optical spectra

NASA Astrophysics Data System (ADS)

Ross, Nicholas P.; Hamann, Fred; Zakamska, Nadia L.; Richards, Gordon T.; Villforth, Carolin; Strauss, Michael A.; Greene, Jenny E.; Alexandroff, Rachael; Brandt, W. Niel; Liu, Guilin; Myers, Adam D.; Pâris, Isabelle; Schneider, Donald P.

2015-11-01

Quasars with extremely red infrared-to-optical colours are an interesting population that can test ideas about quasar evolution as well as orientation, obscuration and geometric effects in the so-called AGN unified model. To identify such a population, we match the quasar catalogues of the Sloan Digital Sky Survey (SDSS), the Baryon Oscillation Spectroscopic Survey (BOSS) to the Wide-Field Infrared Survey Explorer (WISE) to identify quasars with extremely high infrared-to-optical ratios. We identify 65 objects with rAB - W4Vega > 14 mag (i.e. Fν(22 μm)/Fν(r) ≳ 1000). This sample spans a redshift range of 0.28 < z < 4.36 and has a bimodal distribution, with peaks at z ˜ 0.8 and z ˜ 2.5. It includes three z > 2.6 objects that are detected in the W4 band but not W1 or W2 (i.e. `W1W2 dropouts'). The SDSS/BOSS spectra show that the majority of the objects are reddened type 1 quasars, type 2 quasars (both at low and high redshift) or objects with deep low-ionization broad absorption lines (BALs) that suppress the observed r-band flux. In addition, we identify a class of type 1 permitted broad emission-line objects at z ≃ 2-3 which are characterized by emission line rest-frame equivalent widths (REWs) of ≳150 Å, much larger than those of typical quasars. In particular, 55 per cent (45 per cent) of the non-BAL type 1s with measurable C IV in our sample have REW(C IV) > 100 (150) Å, compared to only 5.8 per cent (1.3 per cent) for non-BAL quasars in BOSS. These objects often also have unusual line ratios, such as very high N V/Ly α ratios. These large REWs might be caused by suppressed continuum emission analogous to type 2 quasars; however, there is no obvious mechanism in standard unified models to suppress the continuum without also obscuring the broad emission lines.

Tank 241-AZ-101 Mixer Pump Test Vapor Sampling and Analysis Plan

DOE Office of Scientific and Technical Information (OSTI.GOV)

TEMPLETON, A.M.

2000-03-06

This sampling and analysis plan (SAP) identifies characterization objectives pertaining to sample collection, laboratory analytical evaluation, and reporting requirements for vapor samples obtained during the operation of mixer pumps in tank 241-AZ-101. The primary purpose of the mixer pump test (MPT) is to demonstrate that the two 300 horsepower mixer pumps installed in tank 241-AZ-101 can mobilize the settled sludge so that it can be retrieved for treatment and vitrification. Sampling will be performed in accordance with Tank 241-AZ-101 Mixer Pump Test Data Quality Objective (Banning 1999) and Data Quality Objectives for Regulatory Requirements for Hazardous and Radioactive Air Emissionsmore » Sampling and Analysis (Mulkey 1999). The sampling will verify if current air emission estimates used in the permit application are correct and provide information for future air permit applications.« less

Tank 241-AZ-101 Mixer Pump Test Vapor Sampling and Analysis Plan

DOE Office of Scientific and Technical Information (OSTI.GOV)

TEMPLETON, A.M.

2000-01-31

This sampling and analysis plan (SAP) identifies characterization objectives pertaining to sample collection, laboratory analytical evaluation, and reporting requirements for vapor samples obtained during the operation of mixer pumps in tank 241-AZ-101. The primary purpose of the mixer pump test (MPT) is to demonstrate that the two 300 horsepower mixer pumps installed in tank 241-AZ-101 can mobilize the settled sludge so that it can be retrieved for treatment and vitrification Sampling will be performed in accordance with Tank 241-AZ-101 Mixer Pump Test Data Quality Objective (Banning 1999) and Data Quality Objectives for Regulatory Requirements for Hazardous and Radioactive Air Emissionsmore » Sampling and Analysis (Mulkey 1999). The sampling will verify if current air emission estimates used in the permit application are correct and provide information for future air permit applications.« less

Tank 241-AZ-101 Mixer Pump Test Vapor Sampling and Analysis Plan

DOE Office of Scientific and Technical Information (OSTI.GOV)

TEMPLETON, A.M.

2000-04-10

This sampling and analysis plan (SAP) identifies characterization objectives pertaining to sample collection, laboratory analytical evaluation, and reporting requirements for vapor samples obtained during the operation of mixer pumps in tank 241-AZ-101. The primary purpose of the mixer pump test (MPT) is to demonstrate that the two 300 horsepower mixer pumps installed in tank 241-AZ-101 can mobilize the settled sludge so that it can be retrieved for treatment and vitrification. Sampling will be performed in accordance with Tank 241-AZ-101 Mixer Pump Test Data Quality Objective (Banning 1999) and Data Quality Objectives for Regulatory Requirements for Hazardous and Radioactive Air Emissionsmore » Sampling and Analysis (Mulkey 1999). The sampling will verify if current air emission estimates used in the permit application are correct and provide information for future air permit applications.« less

Identification of Low-Level Product-Related Variants in Filgrastim Products Presently Available in Highly Regulated Markets.

PubMed

Hausberger, Anna; Lamanna, William C; Hartinger, Martin; Seidl, Andreas; Toll, Hansjoerg; Holzmann, Johann

2016-06-01

Filgrastim is a recombinant, non-glycosylated form of human granulocyte colony-stimulating factor, used to stimulate leukocyte proliferation in patients suffering from neutropenia. Since the expiration of patents associated with Amgen's filgrastim biopharmaceutical, Neupogen(®), in 2006, a number of filgrastim products have been marketed; however, a detailed characterization and comparison of variants associated with these products have not been publically reported. The objective of this study was to identify and quantify product-related variants in filgrastim reference products and biosimilars thereof that are presently available in highly regulated markets. In this study, we used intact and top-down mass spectrometry to identify and quantify product-related variants in filgrastim products. Mass spectrometry has become the method of choice for physicochemical characterization of biopharmaceuticals, allowing accurate and sensitive characterization of product-related variants. In addition to modifications ubiquitously present in biopharmaceuticals, such as methionine oxidation and asparagine/glutamine deamidation, we identified six different low-level, product-related variants present in some, but not all, of the tested products. Two variants, an acetylated filgrastim variant and a filgrastim variant containing an additional C-terminal tryptophan extension, are newly identified variants. This study demonstrates that filgrastim products already in widespread clinical use in highly regulated markets differ in low-level, product-related variants present at levels mostly below 1 % relative abundance. This study provides a comprehensive catalog of minor differences between filgrastim products and suggests that the filgrastim product-related variants described here are not clinically relevant when present at low abundance.

Demonstration of innovative monitoring technologies at the Savannah River Integrated Demonstration Site

DOE Office of Scientific and Technical Information (OSTI.GOV)

Rossabi, J.; Jenkins, R.A.; Wise, M.B.

1993-12-31

The Department of Energy`s Office of Technology Development initiated an Integrated Demonstration Program at the Savannah River Site in 1989. The objective of this program is to develop, demonstrate, and evaluate innovative technologies that can improve present-day environmental restoration methods. The Integrated Demonstration Program at SRS is entitled ``Cleanup of Organics in Soils and Groundwater at Non-Arid Sites.`` New technologies in the areas of drilling, characterization, monitoring, and remediation are being demonstrated and evaluated for their technical performance and cost effectiveness in comparison with baseline technologies. Present site characterization and monitoring methods are costly, time-consuming, overly invasive, and often imprecise.more » Better technologies are required to accurately describe the subsurface geophysical and geochemical features of a site and the nature and extent of contamination. More efficient, nonintrusive characterization and monitoring techniques are necessary for understanding and predicting subsurface transport. More reliable procedures are also needed for interpreting monitoring and characterization data. Site characterization and monitoring are key elements in preventing, identifying, and restoring contaminated sites. The remediation of a site cannot be determined without characterization data, and monitoring may be required for 30 years after site closure.« less

Identifying and classifying quality-of-life tools for assessing pressure ulcers after spinal cord injury

PubMed Central

Hitzig, Sander L.; Balioussis, Christina; Nussbaum, Ethne; McGillivray, Colleen F.; Catharine Craven, B.; Noreau, Luc

2013-01-01

Context Although pressure ulcers may negatively influence quality of life (QoL) post-spinal cord injury (SCI), our understanding of how to assess their impact is confounded by conceptual and measurement issues. To ensure that descriptions of pressure ulcer impact are appropriately characterized, measures should be selected according to the domains that they evaluate and the population and pathologies for which they are designed. Objective To conduct a systematic literature review to identify and classify outcome measures used to assess the impact of pressure ulcers on QoL after SCI. Methods Electronic databases (Medline/PubMed, CINAHL, and PsycInfo) were searched for studies published between 1975 and 2011. Identified outcome measures were classified as being either subjective or objective using a QoL model. Results Fourteen studies were identified. The majority of tools identified in these studies did not have psychometric evidence supporting their use in the SCI population with the exception of two objective measures, the Short-Form 36 and the Craig Handicap Assessment and Reporting Technique, and two subjective measures, the Life Situation Questionnaire-Revised and the Ferrans and Powers Quality of Life Index SCI-Version. Conclusion Many QoL outcome tools showed promise in being sensitive to the presence of pressure ulcers, but few of them have been validated for use with SCI. Prospective studies should employ more rigorous methods for collecting data on pressure ulcer severity and location to improve the quality of findings with regard to their impact on QoL. The Cardiff Wound Impact Schedule is a potential tool for assessing impact of pressure ulcers-post SCI. PMID:24090238

Test plan. GCPS task 7, subtask 7.1: IHM development

NASA Technical Reports Server (NTRS)

Greenberg, H. S.

1994-01-01

The overall objective of Task 7 is to identify cost-effective life cycle integrated health management (IHM) approaches for a reusable launch vehicle's primary structure. Acceptable IHM approaches must: eliminate and accommodate faults through robust designs, identify optimum inspection/maintenance periods, automate ground and on-board test and check-out, and accommodate and detect structural faults by providing wide and localized area sensor and test coverage as required. These requirements are elements of our targeted primary structure low cost operations approach using airline-like maintenance by exception philosophies. This development plan will follow an evolutionary path paving the way to the ultimate development of flight-quality production, operations, and vehicle systems. This effort will be focused on maturing the recommended sensor technologies required for localized and wide area health monitoring to a technology readiness level (TRL) of 6 and to establish flight ready system design requirements. The following is a brief list of IHM program objectives: design out faults by analyzing material properties, structural geometry, and load and environment variables and identify failure modes and damage tolerance requirements; design in system robustness while meeting performance objectives (weight limitations) of the reusable launch vehicle primary structure; establish structural integrity margins to preclude the need for test and checkout and predict optimum inspection/maintenance periods through life prediction analysis; identify optimum fault protection system concept definitions combining system robustness and integrity margins established above with cost effective health monitoring technologies; and use coupons, panels, and integrated full scale primary structure test articles to identify, evaluate, and characterize the preferred NDE/NDI/IHM sensor technologies that will be a part of the fault protection system.

On-line database of voltammetric data of immobilized particles for identifying pigments and minerals in archaeometry, conservation and restoration (ELCHER database).

PubMed

Doménech-Carbó, Antonio; Doménech-Carbó, María Teresa; Valle-Algarra, Francisco Manuel; Gimeno-Adelantado, José Vicente; Osete-Cortina, Laura; Bosch-Reig, Francisco

2016-07-13

A web-based database of voltammograms is presented for characterizing artists' pigments and corrosion products of ceramic, stone and metal objects by means of the voltammetry of immobilized particles methodology. Description of the website and the database is provided. Voltammograms are, in most cases, accompanied by scanning electron microphotographs, X-ray spectra, infrared spectra acquired in attenuated total reflectance Fourier transform infrared spectroscopy mode (ATR-FTIR) and diffuse reflectance spectra in the UV-Vis-region. For illustrating the usefulness of the database two case studies involving identification of pigments and a case study describing deterioration of an archaeological metallic object are presented. Copyright © 2016 Elsevier B.V. All rights reserved.

Biomarkers in Scleroderma: Progressing from Association to Clinical Utility.

PubMed

Ligon, Colin; Hummers, Laura K

2016-03-01

Scleroderma is a heterogenous disease characterized by autoimmunity, a characteristic vasculopathy, and often widely varying extents of deep organ fibrosis. Recent advances in the understanding of scleroderma's evolution have improved the ability to identify subgroups of patients with similar prognosis in order to improve risk stratification, enrich clinical trials for patients likely to benefit from specific therapies, and identify promising therapeutic targets for intervention. High-throughput technologies have recently identified fibrotic and inflammatory effectors in scleroderma that exhibit strong prognostic ability and may be tied to disease evolution. Increasingly, the use of collections of assayed circulating proteins and patterns of gene expression in tissue has replaced single-marker investigations in understanding the evolution of scleroderma and in objectively characterizing disease extent. Lastly, identification of shared patterns of disease evolution has allowed classification of patients into latent disease subtypes, which may allow rapid clinical prognostication and targeted management in both clinical and research settings. The concept of biomarkers in scleroderma is expanding to include nontraditional measures of aggregate protein signatures and disease evolution. This review examines the recent advances in biomarkers with a focus on those approaches poised to guide prospective management or themselves serve as quantitative surrogate disease outcomes.

Plant-microbe genomic systems optimization for energy

DOE Office of Scientific and Technical Information (OSTI.GOV)

Hazen, Samuel P.

The overall objective of this project was to identify genetic variation within grasses that results in increased biomass yield and biofuel conversion efficiency. Improving energy crops hinges on identifying the genetic mechanisms underlying traits that benefit energy production. The exploitation of natural variation in plant species is an ideal approach to identify both the traits and the genes of interest in the production of biofuels. The specific goals of this project were to (1) quantify relevant genetic diversity for biofuel feedstock bioconversion efficiency and biomass accumulation, (2) identify genetic loci that control these traits, and (3) characterize genes for improvedmore » energy crop systems. Determining the key genetic contributors influencing biofuel traits is required in order to determine the viability of these traits as targets for improvement; only then will we be able to apply modern breeding practices and genetic engineering for the rapid improvement of feedstocks.« less

Wake Cycle Robustness of the Mars Science Laboratory Flight Software

NASA Technical Reports Server (NTRS)

Whitehill, Robert

2011-01-01

The Mars Science Laboratory (MSL) is a spacecraft being developed by the Jet Propulsion Laboratory (JPL) for the purpose of in-situ exploration on the surface of Mars. The objective of MSL is to explore and quantitatively assess a local region on the Martian surface as a habitat for microbial life, past or present. This objective will be accomplished through the assessment of the biological potential of at least one target environment, the characterization of the geology and geochemistry of the landing region, an investigation of the planetary process relevant to past habitability, and a characterization of surface radiation. For this purpose, MSL incorporates a total of ten scientific instruments for which functions are to include, among others, atmospheric and descent imaging, chemical composition analysis, and radiation measurement. The Flight Software (FSW) system is responsible for all mission phases, including launch, cruise, entry-descent-landing, and surface operation of the rover. Because of the essential nature of flight software to project success, each of the software modules is undergoing extensive testing to identify and correct errors.

Orbital debris issues

NASA Technical Reports Server (NTRS)

Kessler, D. J.

1985-01-01

Man-made orbital debris, identified as a potential hazard to future space activities, is grouped into size categories. At least 79 satellites have broken up in orbit to date and, in combination with exploded rocket casings and antisatellite debris, threaten 10 km/sec collisions with other orbiting platforms. Only 5 percent of the debris is connected to payloads. The total population of orbiting objects over 4 cm in diameter could number as high as 15,000, and at 1 cm in diameter could be 32,000, based on NASA and NORAD studies. NASA has initiated the 10 yr Space Debris Assessment Program to characterize the hazards of orbiting debris, the potential damage to typical spacecraft components, and to identify means of controlling the damage.

From Global Reconnaissance to Sample Return: A Proposal for a Post-2009 Strategy to Follow the Water on Mars

NASA Technical Reports Server (NTRS)

Clifford, S. M.; George, J. A.; Stoker, C. R.; Briggs, G.

2003-01-01

Since the mid-1990's, the stated strategy of the Mars Exploration Program has been to Follow the Water. Although this strategy has been widely publicized, its degree of influence -- and the logic behind its current implementation (as reflected in mission planning, platform and instrument selection, and allocation of spacecraft resources) remains unclear. In response to this concern, we propose an integrated strategy for the post-2009 exploration of Mars that identifies the scientific objectives, rationale, sequence of missions, and specific investigations, that we believe provides the maximum possible science return by pursuing the most direct, cost-effective, and technically capable approach to following the water. This strategy is based on the orbital identification, high-resolution surface investigation, and ultimate sampling of the highest priority targets: near-surface liquid water and massive ground ice (potentially associated with the discharge of the outlflow channels or the relic of a former ocean). The analysis of such samples, in conjunction with the data acquired by the necessary precursor investigations (to identify the locations and characterize the environments of the optimum sampling sites), is expected to address a majority of the goals and high priority science objectives identified by MEPAG.

Geospatial mapping of Antarctic coastal oasis using geographic object-based image analysis and high resolution satellite imagery

NASA Astrophysics Data System (ADS)

Jawak, Shridhar D.; Luis, Alvarinho J.

2016-04-01

An accurate spatial mapping and characterization of land cover features in cryospheric regions is an essential procedure for many geoscientific studies. A novel semi-automated method was devised by coupling spectral index ratios (SIRs) and geographic object-based image analysis (OBIA) to extract cryospheric geospatial information from very high resolution WorldView 2 (WV-2) satellite imagery. The present study addresses development of multiple rule sets for OBIA-based classification of WV-2 imagery to accurately extract land cover features in the Larsemann Hills, east Antarctica. Multilevel segmentation process was applied to WV-2 image to generate different sizes of geographic image objects corresponding to various land cover features with respect to scale parameter. Several SIRs were applied to geographic objects at different segmentation levels to classify land mass, man-made features, snow/ice, and water bodies. We focus on water body class to identify water areas at the image level, considering their uneven appearance on landmass and ice. The results illustrated that synergetic usage of SIRs and OBIA can provide accurate means to identify land cover classes with an overall classification accuracy of ≍97%. In conclusion, our results suggest that OBIA is a powerful tool for carrying out automatic and semiautomatic analysis for most cryospheric remote-sensing applications, and the synergetic coupling with pixel-based SIRs is found to be a superior method for mining geospatial information.

Overview of the Brooklyn traffic real-time ambient pollutant penetration and environmental dispersion (B-TRAPPED) study: theoretical background and model for design of field experiments.

PubMed

Hahn, Intaek; Wiener, Russell W; Richmond-Bryant, Jennifer; Brixey, Laurie A; Henkle, Stacy W

2009-12-01

The Brooklyn traffic real-time ambient pollutant penetration and environmental dispersion (B-TRAPPED) study was a multidisciplinary field research project that investigated the transport, dispersion, and infiltration processes of traffic emission particulate matter (PM) pollutants in a near-highway urban residential area. The urban PM transport, dispersion, and infiltration processes were described mathematically in a theoretical model that was constructed to develop the experimental objectives of the B-TRAPPED study. In the study, simultaneous and continuous time-series PM concentration and meteorological data collected at multiple outdoor and indoor monitoring locations were used to characterize both temporal and spatial patterns of the PM concentration movements within microscale distances (<500 m) from the highway. Objectives of the study included (1) characterizing the temporal and spatial PM concentration fluctuation and distribution patterns in the urban street canyon; (2) investigating the effects of urban structures such as a tall building or an intersection on the transport and dispersion of PM; (3) studying the influence of meteorological variables on the transport, dispersion, and infiltration processes; (4) characterizing the relationships between the building parameters and the infiltration mechanisms; (5) establishing a cause-and-effect relationship between outdoor-released PM and indoor PM concentrations and identifying the dominant mechanisms involved in the infiltration process; (6) evaluating the effectiveness of a shelter-in-place area for protection against outdoor-released PM pollutants; and (7) understanding the predominant airflow and pollutant dispersion patterns within the neighborhood using wind tunnel and CFD simulations. The 10 papers in this first set of papers presenting the results from the B-TRAPPED study address these objectives. This paper describes the theoretical background and models representing the interrelated processes of transport, dispersion, and infiltration. The theoretical solution for the relationship between the time-dependent indoor PM concentration and the initial PM concentration at the outdoor source was obtained. The theoretical models and solutions helped us to identify important parameters in the processes of transport, dispersion, and infiltration. The B-TRAPPED study field experiments were then designed to investigate these parameters in the hope of better understanding urban PM pollutant behaviors.

The nurse as a sex object in motion pictures, 1930 to 1980.

PubMed

Kalisch, B J; Kalisch, P A; McHugh, M L

1982-09-01

A content analysis of 191 motion pictures featuring 211 nurses as significant characters was conducted to determine the nature and extent of the motion picture industry's depiction of the nurse as a sex object and to identify changes in that portrayal from 1930 to 1980. Seventy-three percent of the nurse roles characterized nurses as sex objects. The frequency and intensity of stereotypes of nurses as sex objects rose significantly during the 1960s and 1970s (p less than .0001). Exploitation of the nurse as a sex object was more common in the larger nurse roles. However, in films with a strong emphasis on professional nursing in either the story or in character development, sexual stereotyping of nurses was uncommon. It was concluded that the image of the nurse as a professional care giver was incompatible with that of the nurse as sex object, and that the motion picture industry has opted primarily to present the latter image. The extremely negative sexual stereotype of nursing promulgated during the past 20 years is cause for concern. Actions that the nursing profession can employ to counter the unfavorable portrayal of nurses in 1980s motion pictures are suggested.

Amodal causal capture in the tunnel effect.

PubMed

Bae, Gi Yeul; Flombaum, Jonathan I

2011-01-01

In addition to identifying individual objects in the world, the visual system must also characterize the relationships between objects, for instance when objects occlude one another or cause one another to move. Here we explored the relationship between perceived causality and occlusion. Can one perceive causality in an occluded location? In several experiments, observers judged whether a centrally presented event involved a single object passing behind an occluder, or one object causally launching another (out of view and behind the occluder). With no additional context, the centrally presented event was typically judged as a non-causal pass, even when the occluding and disoccluding objects were different colors--an illusion known as the 'tunnel effect' that results from spatiotemporal continuity. However, when a synchronized context event involved an unambiguous causal launch, participants perceived a causal launch behind the occluder. This percept of an occluded causal interaction could also be driven by grouping and synchrony cues in the absence of any explicitly causal interaction. These results reinforce the hypothesis that causality is an aspect of perception. It is among the interpretations of the world that are independently available to vision when resolving ambiguity, and that the visual system can 'fill in' amodally.

Many-Objective Reservoir Policy Identification and Refinement to Reduce Institutional Myopia in Water Management

NASA Astrophysics Data System (ADS)

Giuliani, M.; Herman, J. D.; Castelletti, A.; Reed, P. M.

2013-12-01

Institutional inertia strongly limits our ability to adapt water reservoir operations to better manage growing water demands as well as their associated uncertainties in a changing climate. Although it has long been recognized that these systems are generally framed in heterogeneous socio-economic contexts involving a myriad of conflicting, non-commensurable operating objectives, our broader understanding of the multiobjective consequences of current operating rules as well as their vulnerability to hydroclimatic uncertainties is severely limited. This study proposes a decision analytic framework to overcome policy inertia and myopia in complex river basin management contexts. The framework combines reservoir policy identification and many-objective optimization under uncertainty to characterize current operations and discover key tradeoffs between alternative policies for balancing evolving demands and system uncertainties. The approach is demonstrated on the Conowingo Dam, located within the Lower Susquehanna River, USA. The Lower Susquehanna River is an interstate water body that has been subject to intensive water management efforts due to the system's competing demands from urban water supply, atomic power plant cooling, hydropower production, and federally regulated environmental flows. Initially our proposed framework uses available streamflow observations to implicitly identify the Conowingo Dam's current but unknown operating policy. This baseline policy is identified by fitting radial basis functions to existing system dynamics. Our assumption in the baseline policy is that the dam operator is represented as a rational agent seeking to maximize primary operational objectives (i.e., guaranteeing the public water supply and maximizing the hydropower revenue). The quality of the identified baseline policy is evaluated by its ability to replicate historical release dynamics. Once identified, the historical baseline policy then provides a means of representing the decision preferences guiding current operations. Our results show that the estimated policy closely captures the dynamics of current releases and flows for the Lower Susquehanna. After identifying the historical baseline policy, our proposed decision analytic framework then combines evolutionary many-objective optimization with visual analytics to discover improved operating policies. Our Lower Susquehanna results confirm that the system's current history-based operations are negatively biased to overestimate the reliability of the reservoir's multi-sector services. Moreover, our proposed framework has successfully identified alternative reservoir policies that are more robust to hydroclimatic uncertainties while being capable of better addressing the tradeoffs across the Conowingo Dam's multi-sector services.

DOE Office of Scientific and Technical Information (OSTI.GOV)

Thirouin, A.; Moskovitz, N.; Burt, B.

The Mission Accessible Near-Earth Objects Survey aims to physically characterize sub-km near-Earth objects (NEOs). We report the first photometric results from the survey that began in 2013 August. Photometric observations were performed using 1–4 m class telescopes around the world. We present rotational periods and light curve amplitudes for 86 sub-km NEOs, though in some cases only lower limits are provided. Our main goal is to obtain light curves for small NEOs (typically, sub-km objects) and estimate their rotational periods, light curve amplitudes, and shapes. These properties are used for a statistical study to constrain overall properties of the NEOmore » population. A weak correlation seems to indicate that smaller objects are more spherical than larger ones. We also report seven NEOs that are fully characterized (light curve and visible spectra) as the most suitable candidates for a future human or robotic mission. Viable mission targets are objects fully characterized, with Δ v {sup NHATS} ≤ 12 km s{sup −1}, and a rotational period P  > 1 hr. Assuming a similar rate of object characterization as reported in this paper, approximately 1230 NEOs need to be characterized in order to find 100 viable mission targets.« less

Multi-objects recognition for distributed intelligent sensor networks

NASA Astrophysics Data System (ADS)

He, Haibo; Chen, Sheng; Cao, Yuan; Desai, Sachi; Hohil, Myron E.

2008-04-01

This paper proposes an innovative approach for multi-objects recognition for homeland security and defense based intelligent sensor networks. Unlike the conventional way of information analysis, data mining in such networks is typically characterized with high information ambiguity/uncertainty, data redundancy, high dimensionality and real-time constrains. Furthermore, since a typical military based network normally includes multiple mobile sensor platforms, ground forces, fortified tanks, combat flights, and other resources, it is critical to develop intelligent data mining approaches to fuse different information resources to understand dynamic environments, to support decision making processes, and finally to achieve the goals. This paper aims to address these issues with a focus on multi-objects recognition. Instead of classifying a single object as in the traditional image classification problems, the proposed method can automatically learn multiple objectives simultaneously. Image segmentation techniques are used to identify the interesting regions in the field, which correspond to multiple objects such as soldiers or tanks. Since different objects will come with different feature sizes, we propose a feature scaling method to represent each object in the same number of dimensions. This is achieved by linear/nonlinear scaling and sampling techniques. Finally, support vector machine (SVM) based learning algorithms are developed to learn and build the associations for different objects, and such knowledge will be adaptively accumulated for objects recognition in the testing stage. We test the effectiveness of proposed method in different simulated military environments.

RESEARCH AREA -- MOBILE SOURCE EMISSIONS (EMISSIONS CHARACTERIZATION AND PREVENTION BRANCH, APPCD, NRMRL)

EPA Science Inventory

The objective of this program is to characterize mobile source emissions which are one of the largest sources of tropospheric ozone precursor emissions (CO, NOx, and volotile organic compounds) in the U.S. The research objective of the Emissions Characterization and Prevention Br...

SPLAT: Using Spectral Indices to Identify and Characterize Ultracool Stars, Brown Dwarfs and Exoplanets in Deep Surveys and as Companions to Nearby Stars

NASA Astrophysics Data System (ADS)

Aganze, Christian; Burgasser, Adam J.; Martin, Eduardo; Konopacky, Quinn; Masters, Daniel C.

2016-06-01

The majority of ultracool dwarf stars and brown dwarfs currently known were identified in wide-field red optical and infrared surveys, enabling measures of the local, typically isolated, population in a relatively shallow (<100 pc radius) volume. Constraining the properties of the wider Galactic population (scale height, radial distribution, Population II sources), and close brown dwarf and exoplanet companions to nearby stars, requires specialized instrumentation, such as high-contrast, coronagraphic spectrometers (e.g., Gemini/GPI, VLT/Sphere, Project 1640); and deep spectral surveys (e.g., HST/WFC3 parallel fields, Euclid). We present a set of quantitative methodologies to identify and robustly characterize sources for these specific populations, based on templates and tools developed as part of the SpeX Prism Library Analysis Toolkit. In particular, we define and characterize specifically-tuned sets spectral indices that optimize selection of cool dwarfs and distinguish rare populations (subdwarfs, young planetary-mass objects) based on low-resolution, limited-wavelength-coverage spectral data; and present a template-matching classification method for these instruments. We apply these techniques to HST/WFC3 parallel fields data in the WISPS and HST-3D programs, where our spectral index set allows high completeness and low contamination for searches of late M, L and T dwarfs to distances out to ~3 kpc.The material presented here is based on work supported by the National Aeronautics and Space Administration under Grant No. NNX15AI75G.

Towards a Transferable UAV-Based Framework for River Hydromorphological Characterization

PubMed Central

González, Rocío Ballesteros; Leinster, Paul; Wright, Ros

2017-01-01

The multiple protocols that have been developed to characterize river hydromorphology, partly in response to legislative drivers such as the European Union Water Framework Directive (EU WFD), make the comparison of results obtained in different countries challenging. Recent studies have analyzed the comparability of existing methods, with remote sensing based approaches being proposed as a potential means of harmonizing hydromorphological characterization protocols. However, the resolution achieved by remote sensing products may not be sufficient to assess some of the key hydromorphological features that are required to allow an accurate characterization. Methodologies based on high resolution aerial photography taken from Unmanned Aerial Vehicles (UAVs) have been proposed by several authors as potential approaches to overcome these limitations. Here, we explore the applicability of an existing UAV based framework for hydromorphological characterization to three different fluvial settings representing some of the distinct ecoregions defined by the WFD geographical intercalibration groups (GIGs). The framework is based on the automated recognition of hydromorphological features via tested and validated Artificial Neural Networks (ANNs). Results show that the framework is transferable to the Central-Baltic and Mediterranean GIGs with accuracies in feature identification above 70%. Accuracies of 50% are achieved when the framework is implemented in the Very Large Rivers GIG. The framework successfully identified vegetation, deep water, shallow water, riffles, side bars and shadows for the majority of the reaches. However, further algorithm development is required to ensure a wider range of features (e.g., chutes, structures and erosion) are accurately identified. This study also highlights the need to develop an objective and fit for purpose hydromorphological characterization framework to be adopted within all EU member states to facilitate comparison of results. PMID:28954434

Towards a Transferable UAV-Based Framework for River Hydromorphological Characterization.

PubMed

Rivas Casado, Mónica; González, Rocío Ballesteros; Ortega, José Fernando; Leinster, Paul; Wright, Ros

2017-09-26

The multiple protocols that have been developed to characterize river hydromorphology, partly in response to legislative drivers such as the European Union Water Framework Directive (EU WFD), make the comparison of results obtained in different countries challenging. Recent studies have analyzed the comparability of existing methods, with remote sensing based approaches being proposed as a potential means of harmonizing hydromorphological characterization protocols. However, the resolution achieved by remote sensing products may not be sufficient to assess some of the key hydromorphological features that are required to allow an accurate characterization. Methodologies based on high resolution aerial photography taken from Unmanned Aerial Vehicles (UAVs) have been proposed by several authors as potential approaches to overcome these limitations. Here, we explore the applicability of an existing UAV based framework for hydromorphological characterization to three different fluvial settings representing some of the distinct ecoregions defined by the WFD geographical intercalibration groups (GIGs). The framework is based on the automated recognition of hydromorphological features via tested and validated Artificial Neural Networks (ANNs). Results show that the framework is transferable to the Central-Baltic and Mediterranean GIGs with accuracies in feature identification above 70%. Accuracies of 50% are achieved when the framework is implemented in the Very Large Rivers GIG. The framework successfully identified vegetation, deep water, shallow water, riffles, side bars and shadows for the majority of the reaches. However, further algorithm development is required to ensure a wider range of features (e.g., chutes, structures and erosion) are accurately identified. This study also highlights the need to develop an objective and fit for purpose hydromorphological characterization framework to be adopted within all EU member states to facilitate comparison of results.

Quantifiable and objective approach to organizational performance enhancement.

DOE Office of Scientific and Technical Information (OSTI.GOV)

Scholand, Andrew Joseph; Tausczik, Yla R.

This report describes a new methodology, social language network analysis (SLNA), that combines tools from social language processing and network analysis to identify socially situated relationships between individuals which, though subtle, are highly influential. Specifically, SLNA aims to identify and characterize the nature of working relationships by processing artifacts generated with computer-mediated communication systems, such as instant message texts or emails. Because social language processing is able to identify psychological, social, and emotional processes that individuals are not able to fully mask, social language network analysis can clarify and highlight complex interdependencies between group members, even when these relationships aremore » latent or unrecognized. This report outlines the philosophical antecedents of SLNA, the mechanics of preprocessing, processing, and post-processing stages, and some example results obtained by applying this approach to a 15-month corporate discussion archive.« less

Social pediatrics: weaving horizontal and vertical threads through pediatric residency.

PubMed

van den Heuvel, Meta; Martimianakis, Maria Athina Tina; Levy, Rebecca; Atkinson, Adelle; Ford-Jones, Elizabeth; Shouldice, Michelle

2017-01-13

Social pediatrics teaches pediatric residents how to understand disease within their patients' social, environmental and political contexts. It's an essential component of pediatric residency training; however there is very little literature that addresses how such a broad-ranging topic can be taught effectively. The aim of this study was to determine and characterize social pediatric education in our pediatric residency training in order to identify strengths and gaps. A social pediatrics curriculum map was developed, attending to 3 different dimensions: (1) the intended curriculum as prescribed by the Objectives of Training for Pediatrics of the Royal College of Physicians and Surgeons of Canada (RCPSC), (2) the formal curriculum defined by rotation-specific learning objectives, and (3) the informal/hidden curriculum as reflected in resident and teacher experiences and perceptions. Forty-one social pediatric learning objectives were extracted from the RCPSC Objectives of Training for Pediatrics, most were listed in the Medical Expert (51%) and Health Advocate competencies (24%). Almost all RCPSC social pediatric learning objectives were identified in more than one rotation and/or seminar. Adolescent Medicine (29.2%), Pediatric Ambulatory Medicine (26.2%) and Developmental Pediatrics (25%) listed the highest proportion of social pediatric learning objectives. Four (10%) RCPSC social pediatric objectives were not explicitly named within learning objectives of the formal curriculum. The informal curriculum revealed that both teachers and residents viewed social pediatrics as integral to all clinical encounters. Perceived barriers to teaching and learning of social pediatrics included time constraints, particularly in a tertiary care environment, and the value of social pediatrics relative to medical expert knowledge. Despite the lack of an explicit thematic presentation of social pediatric learning objectives by the Royal College and residency training program, social pediatric topics are integrated, taught and learned throughout the entire curriculum. Special attention needs to be given to the hidden curriculum and system barriers that may impede social pediatric education.

Exploration of complex visual feature spaces for object perception

PubMed Central

Leeds, Daniel D.; Pyles, John A.; Tarr, Michael J.

2014-01-01

The mid- and high-level visual properties supporting object perception in the ventral visual pathway are poorly understood. In the absence of well-specified theory, many groups have adopted a data-driven approach in which they progressively interrogate neural units to establish each unit's selectivity. Such methods are challenging in that they require search through a wide space of feature models and stimuli using a limited number of samples. To more rapidly identify higher-level features underlying human cortical object perception, we implemented a novel functional magnetic resonance imaging method in which visual stimuli are selected in real-time based on BOLD responses to recently shown stimuli. This work was inspired by earlier primate physiology work, in which neural selectivity for mid-level features in IT was characterized using a simple parametric approach (Hung et al., 2012). To extend such work to human neuroimaging, we used natural and synthetic object stimuli embedded in feature spaces constructed on the basis of the complex visual properties of the objects themselves. During fMRI scanning, we employed a real-time search method to control continuous stimulus selection within each image space. This search was designed to maximize neural responses across a pre-determined 1 cm3 brain region within ventral cortex. To assess the value of this method for understanding object encoding, we examined both the behavior of the method itself and the complex visual properties the method identified as reliably activating selected brain regions. We observed: (1) Regions selective for both holistic and component object features and for a variety of surface properties; (2) Object stimulus pairs near one another in feature space that produce responses at the opposite extremes of the measured activity range. Together, these results suggest that real-time fMRI methods may yield more widely informative measures of selectivity within the broad classes of visual features associated with cortical object representation. PMID:25309408

Using thermal infrared (TIR) data to characterize dust sources, dust fall and the linkage to climate in the Middle East

NASA Astrophysics Data System (ADS)

Mohammad, R.; Ramsey, M.; Scheidt, S. P.

2010-12-01

Prior to mineral dust deposition affecting albedo, aerosols can have direct and indirect effects on local to regional scale climate by changing both the shortwave and longwave radiative forcing. In addition, mineral dust causes health hazards, such as respiratory-related illnesses and deaths, loss of agricultural soil, and safety hazards to aviation and motorists due to reduced visibility. Previous work utilized satellite and ground-based TIR data to describe the direct longwave radiative effect of the Saharan Air Layer (SAL) over the Atlantic Ocean originating from dust storms in the Western Sahara. TIR emission spectroscopy was used to identify the spectral absorption features of that dust. The current research focuses on Kuwait and utilizes a comprehensive set of spatial, analytical and geological tools to characterize dust emissions and its radiative effects. Surface mineral composition maps for the Kuwait region were created using ASTER images and GIS datasets in order to identify the possible sources of wind-blown dust. Backward trajectory analysis using the Hybrid Single-Particle Lagrangian Integrated Trajectory (HYSPLIT) model suggests the dust source areas were located in Iraq, Syria, Jordan and Saudi Arabia. Samples collected from two dust storms (May and July 2010) were analyzed for their mineral composition and to validate the dust source areas identified by the modeling and remote sensing analysis. These air fall dust samples were collected in glass containers on a 13 meter high rooftop in the suburb of Rumaithiya in Kuwait. Additional samples will be collected to expand the analysis and their chemical compositions will be characterized by a combination of laboratory X-ray fluorescence (XRF), Scanning Electron Microscopy (SEM) and TIR emission spectroscopy. The overarching objective of this ongoing research is to both characterize the effects of mineral dust on climate as well as establish a predictive tool that can identify dust storm sources and potentially aid in establishing a more accurate prediction and warning system in the Middle East region.

Clustering of multiple energy balance related behaviors is associated with body fat composition indicators in adolescents: Results from the HELENA and ELANA studies.

PubMed

Moreira, Naiara Ferraz; da Veiga, Gloria Valeria; Santaliestra-Pasías, Alba María; Androutsos, Odysseas; Cuenca-García, Magdalena; de Oliveira, Alessandra Silva Dias; Pereira, Rosangela Alves; de Moraes, Anelise Bezerra de Vasconcelos; Van den Bussche, Karen; Censi, Laura; González-Gross, Marcela; Cañada, David; Gottrand, Frederic; Kafatos, Anthony; Marcos, Ascensión; Widhalm, Kurt; Mólnar, Dénes; Moreno, Luis Alberto

2018-01-01

The objective of this study was to identify clustering patterns of four energy balance-related behaviors (EBRB): television (TV) watching, moderate and vigorous physical activity (MVPA), consumption of fruits and vegetables (F&V), and consumption of sugar-sweetened beverages (SSB), among European and Brazilian adolescents. EBRB associations with different body fat composition indicators were then evaluated. Participants included adolescents from eight European countries in the HELENA (Healthy Lifestyle in Europe by Nutrition in Adolescents) study (n = 2,057, 53.8% female; age: 12.5-17.5 years) and from the metropolitan region of Rio de Janeiro/Brazil in the ELANA study (the Adolescent Nutritional Assessment Longitudinal Study) (n = 968, 53.2% female; age: 13.5-19 years). EBRB data allowed for sex- and study-specific clusters. Associations were estimated by ANOVA and odds ratios. Five clustering patterns were identified. Four similar clusters were identified for each sex and study. Among boys, different cluster identified was characterized by high F&V consumption in the HELENA study and high TV watching and high MVPA time in the ELANA study. Among girls, the different clusters identified was characterized by high F&V consumption in both studies and, additionally, high SSB consumption in the ELANA study. Regression analysis showed that clusters characterized by high SSB consumption in European boys; high TV watching, and high TV watching plus high MVPA in Brazilian boys; and high MVPA, and high SSB and F&V consumption in Brazilian girls, were positively associated with different body fat composition indicators. Common clusters were observed in adolescents from Europe and Brazil, however, no cluster was identified as being completely healthy or unhealthy. Each cluster seems to impact on body composition indicators, depending on the group. Public health actions should aim to promote adequate practices of EBRB. Copyright © 2017. Published by Elsevier Ltd.

Extracting Spatiotemporal Objects from Raster Data to Represent Physical Features and Analyze Related Processes

NASA Astrophysics Data System (ADS)

Zollweg, J. A.

2017-10-01

Numerous ground-based, airborne, and orbiting platforms provide remotely-sensed data of remarkable spatial resolution at short time intervals. However, this spatiotemporal data is most valuable if it can be processed into information, thereby creating meaning. We live in a world of objects: cars, buildings, farms, etc. On a stormy day, we don't see millions of cubes of atmosphere; we see a thunderstorm `object'. Temporally, we don't see the properties of those individual cubes changing, we see the thunderstorm as a whole evolving and moving. There is a need to represent the bulky, raw spatiotemporal data from remote sensors as a small number of relevant spatiotemporal objects, thereby matching the human brain's perception of the world. This presentation reveals an efficient algorithm and system to extract the objects/features from raster-formatted remotely-sensed data. The system makes use of the Python object-oriented programming language, SciPy/NumPy for matrix manipulation and scientific computation, and export/import to the GeoJSON standard geographic object data format. The example presented will show how thunderstorms can be identified and characterized in a spatiotemporal continuum using a Python program to process raster data from NOAA's High-Resolution Rapid Refresh v2 (HRRRv2) data stream.

Characterizing Novel Thermophilic Amylase Producing Bacteria From Taptapani Hot Spring, Odisha, India

PubMed Central

Sen, Sudip Kumar; Raut, Sangeeta; Satpathy, Soumya; Rout, Prangya Ranjan; Bandyopadhyay, Bidyut; Das Mohapatra, Pradeep Kumar

2014-01-01

Background: Amylases play a vital role in biotechnological studies and rank an important position in the world enzyme market (25% to 33%). Bioprocess method of amylase production is more effective than the other sources, since the technique is easy, cost effective, fast, and the enzymes of required properties can be procured. Objectives: The current study aimed to report the characteristics of novel amylase producing bacterial strains isolated from Taptapani hot spring, Odisha, India. Materials and Methods: Bacterial strains were isolated by dilution plating method from the water samples collected from Taptapani Hot Spring, Odisha and screened for amylase production through starch hydrolysis. The bacterial isolates were identified morphologically, biochemically, and finally by 16S rDNA profiling. Results: Based on the morphological, physiological, biochemical characteristics and the molecular characterization, the isolates SS1, SS2, and SS3 were identified as Bacillus barbaricus, Aeromonas veroni, and Stenotrophomonas maltophilia, respectively. The approximate molecular weight of enzymes from SS1, SS2, and SS3 strains were 19 kDa, 56 kDa and 49 kDa, respectively. Conclusions: The current report isolates, characterizes, and demonstrates the novel heat-adapted amylase-producing bacteria SS1, SS2 and SS3 from Taptapani hot spring, indicating its potentiality and stability under acidic conditions. PMID:25741425

Identification Of Minangkabau Landscape Characters

NASA Astrophysics Data System (ADS)

Asrina, M.; Gunawan, A.; Aris, Munandar

2017-10-01

Minangkabau is one of cultures in indonesia which occupies landscape intact. Landscape of Minangkabau have a very close relationship with the culture of the people. Uniqueness of Minangkabau culture and landscape forming an inseparable characterunity. The landscape is necessarily identified to know the inherent landscape characters. The objective of this study was to identify the character of the Minangkabau landscape characterizes its uniqueness. The study was conducted by using descriptive method comprised literature review and field observasion. Observed the landscape characters comprised two main features, they were major and minor features. Indetification of the features was conducted in two original areas (darek) of the Minangkabau traditional society. The research results showed that major features or natural features of the landscape were predominantly landform, landcover, and hidrology. All luhak (districts) of Minangkabau showed similar main features such as hill, canyon, lake, valley, and forest. The existence of natural features such as hills, canyon and valleys characterizes the nature of minangkabau landscape. Minor features formed by Minangkabau cultural society were agricultural land and settlement. Rumah gadang (big house) is one of famous minor features characterizes the Minangkabau culture. In addition, several historical artefacts of building and others structure may strengthen uniqueness of the Minangkabau landscape character, such as The royal palace, inscription, and tunnels.

Mexican-Origin Parents’ Latent Occupational Profiles: Associations with Parent-Youth Relationships and Youth Aspirations

PubMed Central

Wheeler, Lorey A.; Updegraff, Kimberly A.; Umaña-Taylor, Adriana; Tein, Jenn-Yun

2014-01-01

This study utilized an ecological, person-centered approach to identify subgroups of families who had similar profiles across multiple dimensions of Mexican-origin mothers’ and fathers’ occupational characteristics (i.e., self-direction, hazardous conditions, physical activity) and to relate these subgroups to families’ sociocultural characteristics and youth adjustment. The study included 160 dual-earner Mexican-origin families from the urban Southwest. Mothers’ and fathers’ objective work characteristics and families’ sociocultural characteristics were assessed when youth were in early to middle adolescence; adjustment was assessed during late adolescence and early adulthood for two offspring in each family. A latent profile analysis identified 3 profiles that evidenced distinct patterns of occupational characteristics: a differentiated high physical activity profile characterized by high levels of physical activity and low levels of self-direction; an incongruent profile characterized by large differences between parents on self-direction, hazards, and physical activity; and a congruent highly self-directed profile characterized by congruence across parents on occupational characteristics. These profiles were linked to sociocultural characteristics (i.e., family income, educational attainment, and acculturation) and to relational adjustment (i.e., mother- and father-youth conflict, father warmth) and educational aspirations. Results are discussed with respect to implications of parents’ work for youth’s future family relationships and attainment. PMID:23957822

Molecular characterization, biological forms and sporozoite rate of Anopheles stephensi in southern Iran

PubMed Central

Chavshin, Ali Reza; Oshaghi, Mohammad Ali; Vatandoost, Hasan; Hanafi-Bojd, Ahmad Ali; Raeisi, Ahmad; Nikpoor, Fatemeh

2014-01-01

Objective To identify the biological forms, sporozoite rate and molecular characterization of the Anopheles stephensi (An. stephensi) in Hormozgan and Sistan-Baluchistan provinces, the most important malarious areas in Iran. Methods Wild live An. stephensi samples were collected from different malarious areas in southern Iran. The biological forms were identified based on number of egg-ridges. Molecular characterization of biological forms was verified by analysis of the mitochondrial cytochrome oxidase subunit I and II (mtDNA-COI/COII). The Plasmodium infection was examined in the wild female specimens by species-specific nested–PCR method. Results Results showed that all three biological forms including mysorensis, intermediate and type are present in the study areas. Molecular investigations revealed no genetic variation between mtDNA COI/COII sequences of the biological forms and no Plasmodium parasites was detected in the collected mosquito samples. Conclusions Presence of three biological forms with identical sequences showed that the known biological forms belong to a single taxon and the various vectorial capacities reported for these forms are more likely corresponded to other epidemiological factors than to the morphotype of the populations. Lack of malaria parasite infection in An. stephensi, the most important vector of malaria, may be partly due to the success and achievement of ongoing active malaria control program in the region. PMID:24144130

A spectroscopic study of Brazilwood paints in medieval books of hours.

PubMed

Melo, Maria João; Otero, Vanessa; Vitorino, Tatiana; Araújo, Rita; Muralha, Vânia S F; Lemos, Ana; Picollo, Marcello

2014-01-01

In this work, microspectrofluorimetry was for the first time applied to the identification of the red organic lakes that are characteristic of the lavish illuminations found in 15(th) century books of hours. Microspectrofluorimetry identified those red paints, ranging from opaque pink to dark red glazes, as brazilwood lakes. An unequivocal characterization was achieved by comparison with reference paints produced following recipes from the medieval treatise The Book on How to Make Colours, and was further confirmed by fiber optic reflectance spectroscopy (FORS). For these treasured cultural objects, microspectrofluorimetry and FORS proved to be the only techniques that could identify, in situ or in microsamples, the chromophore responsible for the pinkish hues: a brazilein-Al(3+) complex. Additionally, a multi-analytical approach provided a full characterization of the color paints, including pigments, additives, and binders. Microspectroscopic techniques, based on infrared and X-ray radiation, enabled us to disclose the full palette of these medieval manuscripts, including the elusive greens, for which, besides malachite, basic copper sulfates were found; Raman microscopy suggested a mixture of brochantite and langite. Infrared analysis proved invaluable for a full characterization of the additives that were applied as fillers or whites (chalk, gypsum, and white lead) as well as the proteinaceous and polysaccharide binders that were found pure or in mixture.

Strategies for Global Optimization of Temporal Preferences

NASA Technical Reports Server (NTRS)

Morris, Paul; Morris, Robert; Khatib, Lina; Ramakrishnan, Sailesh

2004-01-01

A temporal reasoning problem can often be naturally characterized as a collection of constraints with associated local preferences for times that make up the admissible values for those constraints. Globally preferred solutions to such problems emerge as a result of well-defined operations that compose and order temporal assignments. The overall objective of this work is a characterization of different notions of global preference, and to identify tractable sub-classes of temporal reasoning problems incorporating these notions. This paper extends previous results by refining the class of useful notions of global temporal preference that are associated with problems that admit of tractable solution techniques. This paper also answers the hitherto open question of whether problems that seek solutions that are globally preferred from a Utilitarian criterion for global preference can be found tractably.

Revealing mesoscopic structural universality with diffusion.

PubMed

Novikov, Dmitry S; Jensen, Jens H; Helpern, Joseph A; Fieremans, Els

2014-04-08

Measuring molecular diffusion is widely used for characterizing materials and living organisms noninvasively. This characterization relies on relations between macroscopic diffusion metrics and structure at the mesoscopic scale commensurate with the diffusion length. Establishing such relations remains a fundamental challenge, hindering progress in materials science, porous media, and biomedical imaging. Here we show that the dynamical exponent in the time dependence of the diffusion coefficient distinguishes between the universality classes of the mesoscopic structural complexity. Our approach enables the interpretation of diffusion measurements by objectively selecting and modeling the most relevant structural features. As an example, the specific values of the dynamical exponent allow us to identify the relevant mesoscopic structure affecting MRI-measured water diffusion in muscles and in brain, and to elucidate the structural changes behind the decrease of diffusion coefficient in ischemic stroke.

Identification and genetic characterization of maize cell wall variation for improved biorefinery feedstock characteristics

DOE Office of Scientific and Technical Information (OSTI.GOV)

Pauly, Markus; Hake, Sarah

2013-10-31

The objectives of this program are to 1) characterize novel maize mutants with altered cell walls for enhanced biorefinery characteristics and 2) find quantitative trait loci (QTLs) related to biorefinery characteristics by taking advantage of the genetic diversity of maize. As a result a novel non-transgenic maize plant (cal1) has been identified, whose stover (leaves and stalk) contain more glucan in their walls leading to a higher saccharification yield, when subjected to a standard enzymatic digestion cocktail. Stacking this trait with altered lignin mutants yielded evene higher saccharification yields. Cal-1 mutants do not show a loss of kernel and ormore » biomass yield when grown in the field . Hence, cal1 biomass provides an excellent feedstock for the biofuel industry.« less

Experimental study of canvas characterization for paintings

NASA Astrophysics Data System (ADS)

Cornelis, Bruno; Dooms, Ann; Munteanu, Adrian; Cornelis, Jan; Schelkens, Peter

2010-02-01

The work described here fits in the context of a larger project on the objective and relevant characterization of paintings and painting canvas through the analysis of multimodal digital images. We captured, amongst others, X-ray images of different canvas types, characterized by a variety of textures and weave patterns (fine and rougher texture; single thread and multiple threads per weave), including raw canvas as well as canvas processed with different primers. In this paper, we study how to characterize the canvas by extracting global features such as average thread width, average distance between successive threads (i.e. thread density) and the spatial distribution of primers. These features are then used to construct a generic model of the canvas structure. Secondly, we investigate whether we can identify different pieces of canvas coming from the same bolt. This is an important element for dating, authentication and identification of restorations. Both the global characteristics mentioned earlier and some local properties (such as deviations from the average pattern model) are used to compare the "fingerprint" of different pieces of cloth coming from the same or different bolts.

TTP AL921102: An integrated geophysics program for non-intrusive characterization of mixed-waste landfill sites. FY 1992 year-end progress report: Volume 1

DOE Office of Scientific and Technical Information (OSTI.GOV)

Hasbrouck, J.C.

1992-11-01

Chem-Nuclear Geotech, Inc. (Geotech), operating contractor for the US Department of Energy Grand Junction Projects Office, is conducting the Integrated Geophysics Program for Non-Intrusive Characterization of Mixed-Waste Landfill Sites (Technical Task Plan [TTP] AL921102). The TTP is part of the Mixed-Waste Landfill Integrated Demonstration (MWLID). The objective of this task was to demonstrate that an integrated program of surface geophysics can be used to effectively and nonintrusively characterize n-mixed-waste landfill sites. To accomplish this objective, integrated field demonstrations were conducted over two previously identified areas of interest (designated Areas A and B) within the MWLID test site at the Chemicalmore » Waste Landfill (CWL), Technical Area 3, at the Sandia National Laboratories, Albuquerque, New Mexico (Figures 1 and 2). Area A was centered roughly around the Chromic Acid and Organics Pits in the southeast-central portion of the landfill and Area B was centered around the ``60`s Pits`` area in the northeast-central portion of the landfill. Pit locations were known in Area A and suspected in Area B. This progress report describes the geophysical surveys conducted by Geotech and presents preliminary displays and analyses. Volume 2 of this report contains the raw data for all the surveys conducted by Geotech for this TTP.« less

TTP AL921102: An integrated geophysics program for non-intrusive characterization of mixed-waste landfill sites

DOE Office of Scientific and Technical Information (OSTI.GOV)

Hasbrouck, J.C.

1992-11-01

Chem-Nuclear Geotech, Inc. (Geotech), operating contractor for the US Department of Energy Grand Junction Projects Office, is conducting the Integrated Geophysics Program for Non-Intrusive Characterization of Mixed-Waste Landfill Sites (Technical Task Plan [TTP] AL921102). The TTP is part of the Mixed-Waste Landfill Integrated Demonstration (MWLID). The objective of this task was to demonstrate that an integrated program of surface geophysics can be used to effectively and nonintrusively characterize n-mixed-waste landfill sites. To accomplish this objective, integrated field demonstrations were conducted over two previously identified areas of interest (designated Areas A and B) within the MWLID test site at the Chemicalmore » Waste Landfill (CWL), Technical Area 3, at the Sandia National Laboratories, Albuquerque, New Mexico (Figures 1 and 2). Area A was centered roughly around the Chromic Acid and Organics Pits in the southeast-central portion of the landfill and Area B was centered around the 60's Pits'' area in the northeast-central portion of the landfill. Pit locations were known in Area A and suspected in Area B. This progress report describes the geophysical surveys conducted by Geotech and presents preliminary displays and analyses. Volume 2 of this report contains the raw data for all the surveys conducted by Geotech for this TTP.« less

DOE Office of Scientific and Technical Information (OSTI.GOV)

Gagné, Jonathan; Lafrenière, David; Doyon, René

We present Bayesian Analysis for Nearby Young AssociatioNs II (BANYAN II), a modified Bayesian analysis for assessing the membership of later-than-M5 objects to any of several Nearby Young Associations (NYAs). In addition to using kinematic information (from sky position and proper motion), this analysis exploits 2MASS-WISE color-magnitude diagrams in which old and young objects follow distinct sequences. As an improvement over our earlier work, the spatial and kinematic distributions for each association are now modeled as ellipsoids whose axes need not be aligned with the Galactic coordinate axes, and we use prior probabilities matching the expected populations of the NYAsmore » considered versus field stars. We present an extensive contamination analysis to characterize the performance of our new method. We find that Bayesian probabilities are generally representative of contamination rates, except when a parallax measurement is considered. In this case contamination rates become significantly smaller and hence Bayesian probabilities for NYA memberships are pessimistic. We apply this new algorithm to a sample of 158 objects from the literature that are either known to display spectroscopic signs of youth or have unusually red near-infrared colors for their spectral type. Based on our analysis, we identify 25 objects as new highly probable candidates to NYAs, including a new M7.5 bona fide member to Tucana-Horologium, making it the latest-type member. In addition, we reveal that a known L2γ dwarf is co-moving with a bright M5 dwarf, and we show for the first time that two of the currently known ultra red L dwarfs are strong candidates to the AB Doradus moving group. Several objects identified here as highly probable members to NYAs could be free-floating planetary-mass objects if their membership is confirmed.« less

PLRP-3: Operational Perspectives of Conducting Science-Driven Extravehicular Activity with Communications Latency

NASA Technical Reports Server (NTRS)

Miller, Matthew J.; Lim, Darlene S. S.; Brady, Allyson; Cardman, Zena; Bell, Ernest; Garry, Brent; Reid, Donnie; Chappell, Steve; Abercromby, Andrew F. J.

2016-01-01

The Pavilion Lake Research Project (PLRP) is a unique platform where the combination of scientific research and human space exploration concepts can be tested in an underwater spaceflight analog environment. The 2015 PLRP field season was performed at Pavilion Lake, Canada, where science-driven exploration techniques focusing on microbialite characterization and acquisition were evaluated within the context of crew and robotic extravehicular activity (EVA) operations. The primary objectives of this analog study were to detail the capabilities, decision-making process, and operational concepts required to meet non-simulated scientific objectives during 5-minute one-way communication latency utilizing crew and robotic assets. Furthermore, this field study served as an opportunity build upon previous tests at PLRP, NASA Desert Research and Technology Studies (DRATS), and NASA Extreme Environment Mission Operations (NEEMO) to characterize the functional roles and responsibilities of the personnel involved in the distributed flight control team and identify operational constraints imposed by science-driven EVA operations. The relationship and interaction between ground and flight crew was found to be dependent on the specific scientific activities being addressed. Furthermore, the addition of a second intravehicular operator was found to be highly enabling when conducting science-driven EVAs. Future human spaceflight activities will need to cope with the added complexity of dynamic and rapid execution of scientific priorities both during and between EVA execution to ensure scientific objectives are achieved.

Characterizing the orthodontic patient's purchase decision: A novel approach using netnography.

PubMed

Pittman, Joseph W; Bennett, M Elizabeth; Koroluk, Lorne D; Robinson, Stacey G; Phillips, Ceib L

2017-06-01

A deeper and more thorough characterization of why patients do or do not seek orthodontic treatment is needed for effective shared decision making about receiving treatment. Previous orthodontic qualitative research has identified important dimensions that influence treatment decisions, but our understanding of patients' decisions and how they interpret benefits and barriers of treatment are lacking. The objectives of this study were to expand our current list of decision-making dimensions and to create a conceptual framework to describe the decision-making process. Discussion boards, rich in orthodontic decision-making data, were identified and analyzed with qualitative methods. An iterative process of data collection, dimension identification, and dimension refinement were performed to saturation. A conceptual framework was created to describe the decision-making process. Fifty-four dimensions captured the ideas discussed in regard to a patient's decision to receive orthodontic treatment. Ten domains were identified: function, esthetics, psychosocial benefits, diagnosis, finances, inconveniences, risks of treatment, individual aspects, societal attitudes, and child-specific influences, each containing specific descriptive and conceptual dimensions. A person's desires, self-perceptions, and viewpoints, the public's views on esthetics and orthodontics, and parenting philosophies impacted perceptions of benefits and barriers associated with orthodontic treatment. We identified an expanded list of dimensions, created a conceptual framework describing the orthodontic patient's decision-making process, and identified dimensions associated with yes and no decisions, giving doctors a better understanding of patient attitudes and expectations. Copyright © 2017 American Association of Orthodontists. Published by Elsevier Inc. All rights reserved.

Development of the Chronic Pain Coding System (CPCS) for Characterizing Patient-Clinician Discussions About Chronic Pain and Opioids

PubMed Central

Chen, Meng; Matthias, Marianne S.; Bell, Robert A.; Kravitz, Richard L.

2016-01-01

Objective. To describe the development and initial application of the Chronic Pain Coding System. Design. Secondary analysis of data from a randomized clinical trial. Setting. Six primary care clinics in northern California. Subjects. Forty-five primary care visits involving 33 clinicians and 45 patients on opioids for chronic noncancer pain. Methods. The authors developed a structured coding system to accurately and objectively characterize discussions about pain and opioids. Two coders applied the final system to visit transcripts. Intercoder agreement for major coding categories was moderate to substantial (kappa = 0.5–0.7). Mixed effects regression was used to test six hypotheses to assess preliminary construct validity. Results. Greater baseline pain interference was associated with longer pain discussions (P = 0.007) and more patient requests for clinician action (P = 0.02) but not more frequent negative patient evaluations of pain (P = 0.15). Greater clinician-reported visit difficulty was associated with more frequent disagreements with clinician recommendations (P = 0.003) and longer discussions of opioid risks (P = 0.049) but not more frequent requests for clinician action (P = 0.11). Rates of agreement versus disagreement with patient requests and clinician recommendations were similar for opioid-related and non-opioid–related utterances. Conclusions. This coding system appears to be a reliable and valid tool for characterizing patient-clinician communication about opioids and chronic pain during clinic visits. Objective data on how patients and clinicians discuss chronic pain and opioids are necessary to identify communication patterns and strategies for improving the quality and productivity of discussions about chronic pain that may lead to more effective pain management and reduce inappropriate opioid prescribing. PMID:26936453

Contamination Knowledge Strategy for the Mars 2020 Sample-Collecting Rover

NASA Technical Reports Server (NTRS)

Farley, K. A.; Williford, K.; Beaty, D W.; McSween, H. Y.; Czaja, A. D.; Goreva, Y. S.; Hausrath, E.; Herd, C. D. K.; Humayun, M.; McCubbin, F. M.;

Automatic Detection and Classification of Unsafe Events During Power Wheelchair Use.

PubMed

Pineau, Joelle; Moghaddam, Athena K; Yuen, Hiu Kim; Archambault, Philippe S; Routhier, François; Michaud, François; Boissy, Patrick

2014-01-01

Using a powered wheelchair (PW) is a complex task requiring advanced perceptual and motor control skills. Unfortunately, PW incidents and accidents are not uncommon and their consequences can be serious. The objective of this paper is to develop technological tools that can be used to characterize a wheelchair user's driving behavior under various settings. In the experiments conducted, PWs are outfitted with a datalogging platform that records, in real-time, the 3-D acceleration of the PW. Data collection was conducted over 35 different activities, designed to capture a spectrum of PW driving events performed at different speeds (collisions with fixed or moving objects, rolling on incline plane, and rolling across multiple types obstacles). The data was processed using time-series analysis and data mining techniques, to automatically detect and identify the different events. We compared the classification accuracy using four different types of time-series features: 1) time-delay embeddings; 2) time-domain characterization; 3) frequency-domain features; and 4) wavelet transforms. In the analysis, we compared the classification accuracy obtained when distinguishing between safe and unsafe events during each of the 35 different activities. For the purposes of this study, unsafe events were defined as activities containing collisions against objects at different speed, and the remainder were defined as safe events. We were able to accurately detect 98% of unsafe events, with a low (12%) false positive rate, using only five examples of each activity. This proof-of-concept study shows that the proposed approach has the potential of capturing, based on limited input from embedded sensors, contextual information on PW use, and of automatically characterizing a user's PW driving behavior.

[Tinnitus and psychiatric comorbidities].

PubMed

Goebel, G

2015-04-01

Tinnitus is an auditory phantom phenomenon characterized by the sensation of sounds without objectively identifiable sound sources. To date, its causes are not well understood. The perceived severity of tinnitus correlates more closely to psychological and general health factors than to audiometric parameters. Together with limbic structures in the ventral striatum, the prefrontal cortex forms an internal "noise cancelling system", which normally helps to block out unpleasant sounds, including the tinnitus signal. If this pathway is compromised, chronic tinnitus results. Patients with chronic tinnitus show increased functional connectivity in corticolimbic pathways. Psychiatric comorbidities are common in patients who seek help for tinnitus or hyperacusis. Clinicians need valid screening tools in order to identify patients with psychiatric disorders and to tailor treatment in a multidisciplinary setting.

Characterization and Computational Modeling of Minor Phases in Alloy LSHR

NASA Technical Reports Server (NTRS)

Jou, Herng-Jeng; Olson, Gregory; Gabb, Timothy; Garg, Anita; Miller, Derek

2012-01-01

The minor phases of powder metallurgy disk superalloy LSHR were studied. Samples were consistently heat treated at three different temperatures for long times to approach equilibrium. Additional heat treatments were also performed for shorter times, to assess minor phase kinetics in non-equilibrium conditions. Minor phases including MC carbides, M23C6 carbides, M3B2 borides, and sigma were identified. Their average sizes and total area fractions were determined. CALPHAD thermodynamics databases and PrecipiCalc(TradeMark), a computational precipitation modeling tool, were employed with Ni-base thermodynamics and diffusion databases to model and simulate the phase microstructural evolution observed in the experiments with an objective to identify the model limitations and the directions of model enhancement.

X-ray fluorescence and imaging analyses of paintings by the Brazilian artist Oscar Pereira Da Silva

NASA Astrophysics Data System (ADS)

Campos, P. H. O. V.; Kajiya, E. A. M.; Rizzutto, M. A.; Neiva, A. C.; Pinto, H. P. F.; Almeida, P. A. D.

2014-02-01

Non-destructive analyses, such as EDXRF (Energy-Dispersive X-Ray Fluorescence) spectroscopy, and imaging were used to characterize easel paintings. The analyzed objects are from the collection of the Pinacoteca do Estado de São Paulo. EDXRF results allowed us to identify the chemical elements present in the pigments, showing the use of many Fe-based pigments, modern pigments, such as cobalt blue and cadmium yellow, as well as white pigments containing lead and zinc used by the artist in different layers. Imaging analysis was useful to identify the state of conservation, the localization of old and new restorations and also to detect and unveil the underlying drawings revealing the artist's creative processes.

Observational Progress in Identifying and Characterizing Tidal Disruption Flares

NASA Astrophysics Data System (ADS)

Cenko, S. Bradley

I present an overview of observational efforts across the electromagnetic spectrum to identify and study tidal disruption flares (TDFs), when a star wanders too close to a super-massive black hole and is torn apart by tidal forces. In particular I will focus on four unexpected surprises that challenge the most basic analytic picture of these events: 1) large inferred radii for the optical/UV-emitting material; 2) the ubiquity of outflows, detected at radio, X-ray, and UV wavelengths, ranging from speeds of 100 km/s to near the speed of light; 3) the peculiar atomic abundances observed in the UV and optical spectra of these objects; and, 4) the preference for these events to occur in post-starburst galaxies.

The alfalfa “almost darks” campaign: Pilot VLA HI observations of five high mass-to-light ratio systems

DOE Office of Scientific and Technical Information (OSTI.GOV)

Cannon, John M.; Martinkus, Charlotte P.; Leisman, Lukas

We present new Very Large Array (VLA) H i spectral line imaging of five sources discovered by the ALFALFA extragalactic survey. These targets are drawn from a larger sample of systems that were not uniquely identified with optical counterparts during ALFALFA processing, and as such have unusually high H i mass to light ratios. The candidate “Almost Dark” objects fall into four broad categories: (1) objects with nearby H i neighbors that are likely of tidal origin; (2) objects that appear to be part of a system of multiple H i sources, but which may not be tidal in origin;more » (3) objects isolated from nearby ALFALFA H i detections, but located near a gas-poor early type galaxy; (4) apparently isolated sources, with no object of coincident redshift within ∼400 kpc. Roughly 75% of the 200 objects without identified counterparts in the α.40 database (Haynes et al. 2011) fall into category 1 (likely tidal), and were not considered for synthesis follow-up observations. The pilot sample presented here (AGC193953, AGC208602, AGC208399, AGC226178, and AGC233638) contains the first five sources observed as part of a larger effort to characterize H i sources with no readily identifiable optical counterpart at single dish resolution (3.′5). These objects span a range of H i mass [7.41 < log(M{sub Hi}) < 9.51] and H i mass to B-band luminosity ratios (3 < M{sub Hi}/L{sub B} < 9). We compare the H i total intensity and velocity fields to optical imaging drawn from the Sloan Digital Sky Survey and to ultraviolet imaging drawn from archival GALEX observations. Four of the sources with uncertain or no optical counterpart in the ALFALFA data are identified with low surface brightness optical counterparts in Sloan Digital Sky Survey imaging when compared with VLA H i intensity maps, and appear to be galaxies with clear signs of ordered rotation in the H i velocity fields. Three of these are detected in far-ultraviolet GALEX images, a likely indication of star formation within the last few hundred Myrs. One source (AGC208602) is likely tidal in nature, associated with the NGC 3370 group. Consistent with previous efforts, we find no “dark galaxies” in this limited sample. However, the present observations do reveal complex sources with suppressed star formation, highlighting both the observational difficulties and the necessity of synthesis follow-up observations to understand these extreme objects.« less

Full-length genomic and molecular characterization of Canine parvovirus in dogs from North of Brazil.

PubMed

Silva, S P; Silva, L N P P; Rodrigues, E D L; Cardoso, J F; Tavares, F N; Souza, W M; Santos, C M P; Martins, F M S; Jesus, I S; Brito, T C; Moura, T P C; Nunes, M R T; Casseb, L M N; Silva Filho, E; Casseb, A R

2017-09-21

With the objective of characterizing Canine parvovirus (CPV) from some suspected fecal samples of dogs collected from the Veterinarian Hospital in Belém city, five positive samples were found by PCR assay and an update molecular characterization was provided of the CPV-2 circulation in Belém. Through sequencing of the complete DNA sequences (NS1, NS2, VP1, and VP2 genes), the CPV-2 strain was identified as CPV-2b (Asn426Asp) circulating in Belém. The CPV-2b strain with a different change at the position Tyr324Leu was detected in all samples assessed and thus reported for the first time for the scientific community. Phylogenetic analysis indicated that Belém CPV-2b and CPV-2a strains would be related to a cluster with samples after the 1990s, suggesting that CPV-2b in Belém originated from CPV-2a circulating in Brazil after the 1990s. Potential recombination events were analyzed using RDP4 and SplitsTree4; therefore, results suggest that CPV-2 sequences here described were not potentially recombination events. Continuous monitoring and molecular characterization of CPV-2 samples are needed not only to identify possible genetic and antigenic changes that may interfere with the effectiveness of vaccines but also to bring a better understanding of the mechanisms that drive the evolution of CPV-2 in Brazil.

The Analysis of the General Performance and Mechanical Behavior of Unirradiated FeCrAl Alloys Before and After Welding

DOE Office of Scientific and Technical Information (OSTI.GOV)

Gussev, Maxim N.; Field, Kevin G.; Yamamoto, Yukinori

2016-06-03

The present report summarizes and discusses the preliminary results for the in-depth characterization of the modern, nuclear-grade FeCrAl alloys currently under development. The alloys were designed for enhanced radiation tolerance and weldability, and the research is currently being pursued by the Department of Energy (DOE) Nuclear Energy Enabling Technologies (NEET) program. Last year, seven candidate FeCrAl alloys with well-controlled chemistry and microstructures were designed and produced; welding was performed under well-controlled conditions. The structure and general performance of unirradiated alloys were assessed using standardized and advanced microstructural characterization techniques and mechanical testing. The primary objective is to identify the bestmore » candidate alloy, or at a minimum to identify the contributing factors that increase the weldability and radiation tolerance of FeCrAl alloys, therefore enabling future generations of FeCrAl alloys to deliver better performance parameters. This report is structured so as to describe these critical assessments of the weldability; radiation tolerance will be reported on in later reports from this program.« less

Cardiogenic Genes Expressed in Cardiac Fibroblasts Contribute to Heart Development and Repair

PubMed Central

Furtado, Milena B.; Costa, Mauro W.; Pranoto, Edward Adi; Salimova, Ekaterina; Pinto, Alex; Lam, Nicholas T.; Park, Anthony; Snider, Paige; Chandran, Anjana; Harvey, Richard P.; Boyd, Richard; Conway, Simon J.; Pearson, James; Kaye, David M.; Rosenthal, Nadia A.

2014-01-01

Rationale Cardiac fibroblasts are critical to proper heart function through multiple interactions with the myocardial compartment but appreciation of their contribution has suffered from incomplete characterization and lack of cell-specific markers. Objective To generate an unbiased comparative gene expression profile of the cardiac fibroblast pool, identify and characterize the role of key genes in cardiac fibroblast function, and determine their contribution to myocardial development and regeneration. Methods and Results High-throughput cell surface and intracellular profiling of cardiac and tail fibroblasts identified canonical MSC and a surprising number of cardiogenic genes, some expressed at higher levels than in whole heart. Whilst genetically marked fibroblasts contributed heterogeneously to interstitial but not cardiomyocyte compartments in infarcted hearts, fibroblast-restricted depletion of one highly expressed cardiogenic marker, Tbx20, caused marked myocardial dysmorphology and perturbations in scar formation upon myocardial infarction. Conclusions The surprising transcriptional identity of cardiac fibroblasts, the adoption of cardiogenic gene programs and direct contribution to cardiac development and repair provokes alternative interpretations for studies on more specialized cardiac progenitors, offering a novel perspective for reinterpreting cardiac regenerative therapies. PMID:24650916

Vadose Zone Transport Field Study: Detailed Test Plan for Simulated Leak Tests

DOE Office of Scientific and Technical Information (OSTI.GOV)

Ward, Anderson L.; Gee, Glendon W.

2000-06-23

This report describes controlled transport experiments at well-instrumented field tests to be conducted during FY 2000 in support of DOE?s Vadose Zone Transport Field Study (VZTFS). The VZTFS supports the Groundwater/Vadose Zone Integration Project Science and Technology Initiative. The field tests will improve understanding of field-scale transport and lead to the development or identification of efficient and cost-effective characterization methods. These methods will capture the extent of contaminant plumes using existing steel-cased boreholes. Specific objectives are to 1) identify mechanisms controlling transport processes in soils typical of the hydrogeologic conditions of Hanford?s waste disposal sites; 2) reduce uncertainty in conceptualmore » models; 3) develop a detailed and accurate data base of hydraulic and transport parameters for validation of three-dimensional numerical models; and 4) identify and evaluate advanced, cost-effective characterization methods with the potential to assess changing conditions in the vadose zone, particularly as surrogates of currently undetectable high-risk contaminants. Pacific Northwest National Laboratory (PNNL) manages the VZTFS for DOE.« less

Non-invasive characterization of colorants by portable diffuse reflectance infrared Fourier transform (DRIFT) spectroscopy and chemometrics

NASA Astrophysics Data System (ADS)

Manfredi, Marcello; Barberis, Elettra; Aceto, Maurizio; Marengo, Emilio

2017-06-01

During the last years the need for non-invasive and non-destructive analytical methods brought to the development and application of new instrumentation and analytical methods for the in-situ analysis of cultural heritage objects. In this work we present the application of a portable diffuse reflectance infrared Fourier transform (DRIFT) method for the non-invasive characterization of colorants prepared according to ancient recipes and using egg white and Gum Arabic as binders. Approximately 50 colorants were analyzed with the DRIFT spectroscopy: we were able to identify and discriminate the most used yellow (i.e. yellow ochres, Lead-tin Yellow, Orpiment, etc.), red (i.e. red ochres, Hematite) and blue (i.e. Lapis Lazuli, Azurite, indigo) colorants, creating a complete DRIFT spectral library. The Principal Component Analysis-Discriminant Analysis (PCA-DA) was then employed for the colorants classification according to the chemical/mineralogical composition. The DRIFT analysis was also performed on a gouache painting of the artist Sutherland; and the colorants used by the painter were identified directly in-situ and in a non-invasive manner.

Questions for Surveys

PubMed Central

Schaeffer, Nora Cate; Dykema, Jennifer

2011-01-01

We begin with a look back at the field to identify themes of recent research that we expect to continue to occupy researchers in the future. As part of this overview, we characterize the themes and topics examined in research about measurement and survey questions published in Public Opinion Quarterly in the past decade. We then characterize the field more broadly by highlighting topics that we expect to continue or to grow in importance, including the relationship between survey questions and the total survey error perspective, cognitive versus interactional approaches, interviewing practices, mode and technology, visual aspects of question design, and culture. Considering avenues for future research, we advocate for a decision-oriented framework for thinking about survey questions and their characteristics. The approach we propose distinguishes among various aspects of question characteristics, including question topic, question type and response dimension, conceptualization and operationalization of the target object, question structure, question form, response categories, question implementation, and question wording. Thinking about question characteristics more systematically would allow study designs to take into account relationships among these characteristics and identify gaps in current knowledge. PMID:24970951

Structural and configurational properties of nanoconfined monolayer ice from first principles

PubMed Central

Corsetti, Fabiano; Matthews, Paul; Artacho, Emilio

2016-01-01

Understanding the structural tendencies of nanoconfined water is of great interest for nanoscience and biology, where nano/micro-sized objects may be separated by very few layers of water. Here we investigate the properties of ice confined to a quasi-2D monolayer by a featureless, chemically neutral potential, in order to characterize its intrinsic behaviour. We use density-functional theory simulations with a non-local van der Waals density functional. An ab initio random structure search reveals all the energetically competitive monolayer configurations to belong to only two of the previously-identified families, characterized by a square or honeycomb hydrogen-bonding network, respectively. We discuss the modified ice rules needed for each network, and propose a simple point dipole 2D lattice model that successfully explains the energetics of the square configurations. All identified stable phases for both networks are found to be non-polar (but with a topologically non-trivial texture for the square) and, hence, non-ferroelectric, in contrast to previous predictions from a five-site empirical force-field model. Our results are in good agreement with very recently reported experimental observations. PMID:26728125

Structural and configurational properties of nanoconfined monolayer ice from first principles

NASA Astrophysics Data System (ADS)

Corsetti, Fabiano; Matthews, Paul; Artacho, Emilio

2016-01-01

Understanding the structural tendencies of nanoconfined water is of great interest for nanoscience and biology, where nano/micro-sized objects may be separated by very few layers of water. Here we investigate the properties of ice confined to a quasi-2D monolayer by a featureless, chemically neutral potential, in order to characterize its intrinsic behaviour. We use density-functional theory simulations with a non-local van der Waals density functional. An ab initio random structure search reveals all the energetically competitive monolayer configurations to belong to only two of the previously-identified families, characterized by a square or honeycomb hydrogen-bonding network, respectively. We discuss the modified ice rules needed for each network, and propose a simple point dipole 2D lattice model that successfully explains the energetics of the square configurations. All identified stable phases for both networks are found to be non-polar (but with a topologically non-trivial texture for the square) and, hence, non-ferroelectric, in contrast to previous predictions from a five-site empirical force-field model. Our results are in good agreement with very recently reported experimental observations.

Extravehicular Mobility Unit Training Suit Symptom Study Report

NASA Technical Reports Server (NTRS)

Strauss, Samuel

2004-01-01

The purpose of this study was to characterize the symptoms and injuries experienced by NASA astronauts during extravehicular activity (space walk) spacesuit training at the Neutral Buoyancy Laboratory at Ellington Field, Houston, Texas. We identified the frequency and incidence rates of symptoms by each general body location and characterized mechanisms of injury and effective countermeasures. Based on these findings a comprehensive list of recommendations was made to improve training, test preparation, and current spacesuit components, and to design the next -generation spacesuit. At completion of each test event a comprehensive questionnaire was produced that documented suit symptom comments, identified mechanisms of injury, and recommended countermeasures. As we completed our study we found that most extravehicular mobility unit suit symptoms were mild, self-limited, and controlled by available countermeasures. Some symptoms represented the potential for significant injury with short- and long-term consequences regarding astronaut health and interference with mission objectives. The location of symptoms and injuries that were most clinically significant was in the hands, shoulders, and feet. Correction of suit symptoms issues will require a multidisciplinary approach to improve prevention, early medical intervention, astronaut training, test planning, and suit engineering.

Gold and electrum jewellery in the strategic area of Gadir in Phoenician period

NASA Astrophysics Data System (ADS)

Ortega-Feliu, I.; Gómez-Tubío, B.; Ontalba Salamanca, M. Á.; Respaldiza, M. Á.; de la Bandera, M. L.; Ovejero Zappino, G.

2007-07-01

A set of ancient gold jewellery was found in Cádiz (formerly Gadir, south Spain) in tombs dated in Phoenician-Archaic period (VII-VI century BC), and nowadays is exhibited in the local Museum. The production of this strategic area is of great interest for the knowledge of the commercial routes along the Mediterranean Sea at that time. Part of this production has already been analyzed by the authors, finding compositional differences and identifying soldering procedures, thanks to the use of the external microbeam. Absolutely non destructive analysis was performed. For this work we have again employed PIXE spectrometry with 2.2 MeV protons from the 3 MV Pelletron accelerator at the CNA to characterize the metallic alloys and the manufacturing techniques. We have found an unusual composition characterized by around 50 wt.% gold, 50 wt.% silver and some copper, which can be identified as ELECTRUM. Few analytical data of this particular kind of alloy are reported in the bibliography. The study of these objects can help to follow the trade of metals in the Phoenician-colonial period.

Department of Defense operational applications of wind measurements

NASA Technical Reports Server (NTRS)

Ramsay, Allan C.

1985-01-01

A stated objective for this symposium is to identify requirements for global wind measurements. This paper will draw from recent reports which considered the impact of over 100 environmental factors known to affect military operations. A conclusion that can be drawn from those analyses is that one environmental factor, atmospheric wind, has an operational impact on each of the 48 mission areas examined. This paper will characterize the impact of wind on the various mission areas and will define and summarize both 'technical' and 'operational' requirements for wind intelligence.

Application of SIR-C SAR to Hydrology

NASA Technical Reports Server (NTRS)

Engman, Edwin T.; ONeill, Peggy; Wood, Eric; Pauwels, Valentine; Hsu, Ann; Jackson, Tom; Shi, J. C.; Prietzsch, Corinna

1996-01-01

The progress, results and future plans regarding the following objectives are presented: (1) Determine and compare soil moisture patterns within one or more humid watersheds using SAR data, ground-based measurements, and hydrologic modeling; (2) Use radar data to characterize the hydrologic regime within a catchment and to identify the runoff producing characteristics of humid zone watersheds; and (3) Use radar data as the basis for scaling up from small scale, near-point process models to larger scale water balance models necessary to define and quantify the land phase of GCM's (Global Circulation Models).

Spatial Relationships of Auroral Particle Acceleration Relative to High Latitude Plasma Boundaries

NASA Technical Reports Server (NTRS)

Ghielmetti, Arthur G.

1997-01-01

This final report describes the activities under NASA contract to Lockheed Missiles and Space Company. It covers the period from 10-1-94 to 12-31-97. The objective of this investigation is to identify and characterize the spatial relationships of auroral particle acceleration features relative to the characteristic transition features in the surrounding polar ionospheric plasmas. Due to the reduced funding level approved for this contract, the original scope of the proposed work was readjusted with the focus placed on examining spatial relationships with respect to particle structures.

Developing a taxonomy for mission architecture definition

NASA Technical Reports Server (NTRS)

Neubek, Deborah J.

1990-01-01

The Lunar and Mars Exploration Program Office (LMEPO) was tasked to define candidate architectures for the Space Exploration Initiative to submit to NASA senior management and an externally constituted Outreach Synthesis Group. A systematic, structured process for developing, characterizing, and describing the alternate mission architectures, and applying this process to future studies was developed. The work was done in two phases: (1) national needs were identified and categorized into objectives achievable by the Space Exploration Initiative; and (2) a program development process was created which both hierarchically and iteratively describes the program planning process.

Energy Savings Potential and RD&D Opportunities for Commercial Building Appliances (2015 Update)

DOE Office of Scientific and Technical Information (OSTI.GOV)

Goetzler, William; Guernsey, Matt; Foley, Kevin

The Department of Energy commissioned a technology characterization and assessment of appliances used in commercial buildings for cooking, cleaning, water heating, and other end-uses. The primary objectives of this study were to document the energy consumed by commercial appliances and identify research, development, and demonstration opportunities to improve energy efficiency in each end-use. This report serves as an update to a 2009 report of the same name by incorporating updated data and sources where possible and updating the available technology options that provide opportunities for efficiency improvements.

Cluster analysis of spontaneous preterm birth phenotypes identifies potential associations among preterm birth mechanisms

PubMed Central

Esplin, M Sean; Manuck, Tracy A.; Varner, Michael W.; Christensen, Bryce; Biggio, Joseph; Bukowski, Radek; Parry, Samuel; Zhang, Heping; Huang, Hao; Andrews, William; Saade, George; Sadovsky, Yoel; Reddy, Uma M.; Ilekis, John

2015-01-01

Objective We sought to employ an innovative tool based on common biological pathways to identify specific phenotypes among women with spontaneous preterm birth (SPTB), in order to enhance investigators' ability to identify to highlight common mechanisms and underlying genetic factors responsible for SPTB. Study Design A secondary analysis of a prospective case-control multicenter study of SPTB. All cases delivered a preterm singleton at SPTB ≤34.0 weeks gestation. Each woman was assessed for the presence of underlying SPTB etiologies. A hierarchical cluster analysis was used to identify groups of women with homogeneous phenotypic profiles. One of the phenotypic clusters was selected for candidate gene association analysis using VEGAS software. Results 1028 women with SPTB were assigned phenotypes. Hierarchical clustering of the phenotypes revealed five major clusters. Cluster 1 (N=445) was characterized by maternal stress, cluster 2 (N=294) by premature membrane rupture, cluster 3 (N=120) by familial factors, and cluster 4 (N=63) by maternal comorbidities. Cluster 5 (N=106) was multifactorial, characterized by infection (INF), decidual hemorrhage (DH) and placental dysfunction (PD). These three phenotypes were highly correlated by Chi-square analysis [PD and DH (p<2.2e-6); PD and INF (p=6.2e-10); INF and DH (p=0.0036)]. Gene-based testing identified the INS (insulin) gene as significantly associated with cluster 3 of SPTB. Conclusion We identified 5 major clusters of SPTB based on a phenotype tool and hierarchal clustering. There was significant correlation between several of the phenotypes. The INS gene was associated with familial factors underlying SPTB. PMID:26070700

Neural representations of the concepts in simple sentences: Concept activation prediction and context effects.

PubMed

Just, Marcel Adam; Wang, Jing; Cherkassky, Vladimir L

2017-08-15

Although it has been possible to identify individual concepts from a concept's brain activation pattern, there have been significant obstacles to identifying a proposition from its fMRI signature. Here we demonstrate the ability to decode individual prototype sentences from readers' brain activation patterns, by using theory-driven regions of interest and semantic properties. It is possible to predict the fMRI brain activation patterns evoked by propositions and words which are entirely new to the model with reliably above-chance rank accuracy. The two core components implemented in the model that reflect the theory were the choice of intermediate semantic features and the brain regions associated with the neurosemantic dimensions. This approach also predicts the neural representation of object nouns across participants, studies, and sentence contexts. Moreover, we find that the neural representation of an agent-verb-object proto-sentence is more accurately characterized by the neural signatures of its components as they occur in a similar context than by the neural signatures of these components as they occur in isolation. Copyright © 2017 Elsevier Ltd. All rights reserved.

Bayesian Spatial Design of Optimal Deep Tubewell Locations in Matlab, Bangladesh.

PubMed

Warren, Joshua L; Perez-Heydrich, Carolina; Yunus, Mohammad

2013-09-01

We introduce a method for statistically identifying the optimal locations of deep tubewells (dtws) to be installed in Matlab, Bangladesh. Dtw installations serve to mitigate exposure to naturally occurring arsenic found at groundwater depths less than 200 meters, a serious environmental health threat for the population of Bangladesh. We introduce an objective function, which incorporates both arsenic level and nearest town population size, to identify optimal locations for dtw placement. Assuming complete knowledge of the arsenic surface, we then demonstrate how minimizing the objective function over a domain favors dtws placed in areas with high arsenic values and close to largely populated regions. Given only a partial realization of the arsenic surface over a domain, we use a Bayesian spatial statistical model to predict the full arsenic surface and estimate the optimal dtw locations. The uncertainty associated with these estimated locations is correctly characterized as well. The new method is applied to a dataset from a village in Matlab and the estimated optimal locations are analyzed along with their respective 95% credible regions.

Modeling the tagged-neutron UXO identification technique using the Geant4 toolkit

DOE Office of Scientific and Technical Information (OSTI.GOV)

Zhou Y.; Mitra S.; Zhu X.

2011-10-16

It is proposed to use 14 MeV neutrons tagged by the associated particle neutron time-of-flight technique (APnTOF) to identify the fillers of unexploded ordnances (UXO) by characterizing their carbon, nitrogen and oxygen contents. To facilitate the design and construction of a prototype system, a preliminary simulation model was developed, using the Geant4 toolkit. This work established the toolkit environment for (a) generating tagged neutrons, (b) their transport and interactions within a sample to induce emission and detection of characteristic gamma-rays, and (c) 2D and 3D-image reconstruction of the interrogated object using the neutron and gamma-ray time-of-flight information. Using the modeling,more » this article demonstrates the novelty of the tagged-neutron approach for extracting useful signals with high signal-to-background discrimination of an object-of-interest from that of its environment. Simulations indicated that an UXO filled with the RDX explosive, hexogen (C{sub 3}H{sub 6}O{sub 6}N{sub 6}), can be identified to a depth of 20 cm when buried in soil.« less

Selection and application of microbial source tracking tools for water-quality investigations

USGS Publications Warehouse

Stoeckel, Donald M.

2005-01-01

Microbial source tracking (MST) is a complex process that includes many decision-making steps. Once a contamination problem has been defined, the potential user of MST tools must thoroughly consider study objectives before deciding upon a source identifier, a detection method, and an analytical approach to apply to the problem. Regardless of which MST protocol is chosen, underlying assumptions can affect the results and interpretation. It is crucial to incorporate tests of those assumptions in the study quality-control plan to help validate results and facilitate interpretation. Detailed descriptions of MST objectives, protocols, and assumptions are provided in this report to assist in selection and application of MST tools for water-quality investigations. Several case studies illustrate real-world applications of MST protocols over a range of settings, spatial scales, and types of contamination. Technical details of many available source identifiers and detection methods are included as appendixes. By use of this information, researchers should be able to formulate realistic expectations for the information that MST tools can provide and, where possible, successfully execute investigations to characterize sources of fecal contamination to resource waters.

Eight new Milky Way companions discovered in first-year Dark Energy Survey data

DOE PAGES

Bechtol, K.

2015-06-30

We report the discovery of eight new Milky Way companions inmore » $$\\sim 1800\\;{\\mathrm{deg}}^{2}$$ of optical imaging data collected during the first year of the Dark Energy Survey (DES). Each system is identified as a statistically significant over-density of individual stars consistent with the expected isochrone and luminosity function of an old and metal-poor stellar population. The objects span a wide range of absolute magnitudes (MV from $-2.2$ to $$-7.4\\;\\mathrm{mag}$$), physical sizes ($$10-170\\;\\mathrm{pc}$$), and heliocentric distances ($$30-330\\;\\mathrm{kpc}$$). Based on the low surface brightnesses, large physical sizes, and/or large Galactocentric distances of these objects, several are likely to be new ultra-faint satellite galaxies of the Milky Way and/or Magellanic Clouds. We introduce a likelihood-based algorithm to search for and characterize stellar over-densities, as well as identify stars with high satellite membership probabilities. As a result, we also present completeness estimates for detecting ultra-faint galaxies of varying luminosities, sizes, and heliocentric distances in the first-year DES data.« less

Combined optical sizing and acoustical characterization of single freely-floating microbubbles

NASA Astrophysics Data System (ADS)

Luan, Ying; Renaud, Guillaume; Raymond, Jason L.; Segers, Tim; Lajoinie, Guillaume; Beurskens, Robert; Mastik, Frits; Kokhuis, Tom J. A.; van der Steen, Antonius F. W.; Versluis, Michel; de Jong, Nico

2016-12-01

In this study we present a combined optical sizing and acoustical characterization technique for the study of the dynamics of single freely-floating ultrasound contrast agent microbubbles exposed to long burst ultrasound excitations up to the milliseconds range. A co-axial flow device was used to position individual microbubbles on a streamline within the confocal region of three ultrasound transducers and a high-resolution microscope objective. Bright-field images of microbubbles passing through the confocal region were captured using a high-speed camera synchronized to the acoustical data acquisition to assess the microbubble response to a 1-MHz ultrasound burst. Nonlinear bubble vibrations were identified at a driving pressure as low as 50 kPa. The results demonstrate good agreement with numerical simulations based on the shell-buckling model proposed by Marmottant et al. [J. Acoust. Soc. Am. 118, 3499-3505 (2005)]. The system demonstrates the potential for a high-throughput in vitro characterization of individual microbubbles.

Probabilistic Exposure Analysis for Chemical Risk Characterization

PubMed Central

Bogen, Kenneth T.; Cullen, Alison C.; Frey, H. Christopher; Price, Paul S.

2009-01-01

This paper summarizes the state of the science of probabilistic exposure assessment (PEA) as applied to chemical risk characterization. Current probabilistic risk analysis methods applied to PEA are reviewed. PEA within the context of risk-based decision making is discussed, including probabilistic treatment of related uncertainty, interindividual heterogeneity, and other sources of variability. Key examples of recent experience gained in assessing human exposures to chemicals in the environment, and other applications to chemical risk characterization and assessment, are presented. It is concluded that, although improvements continue to be made, existing methods suffice for effective application of PEA to support quantitative analyses of the risk of chemically induced toxicity that play an increasing role in key decision-making objectives involving health protection, triage, civil justice, and criminal justice. Different types of information required to apply PEA to these different decision contexts are identified, and specific PEA methods are highlighted that are best suited to exposure assessment in these separate contexts. PMID:19223660

Student generated learning objectives: extent of congruence with faculty set objectives and factors influencing their generation.

PubMed

Abdul Ghaffar Al-Shaibani, Tarik A; Sachs-Robertson, Annette; Al Shazali, Hafiz O; Sequeira, Reginald P; Hamdy, Hosam; Al-Roomi, Khaldoon

2003-07-01

A problem-based learning strategy is used for curriculum planning and implementation at the Arabian Gulf University, Bahrain. Problems are constructed in a way that faculty-set objectives are expected to be identified by students during tutorials. Students in small groups, along with a tutor functioning as a facilitator, identify learning issues and define their learning objectives. We compared objectives identified by student groups with faculty-set objectives to determine extent of congruence, and identified factors that influenced students' ability at identifying faculty-set objectives. Male and female students were segregated and randomly grouped. A faculty tutor was allocated for each group. This study was based on 13 problems given to entry-level medical students. Pooled objectives of these problems were classified into four categories: structural, functional, clinical and psychosocial. Univariate analysis of variance was used for comparison, and a p > 0.05 was considered significant. The mean of overall objectives generated by the students was 54.2%, for each problem. Students identified psychosocial learning objectives more readily than structural ones. Female students identified more psychosocial objectives, whereas male students identified more of structural objectives. Tutor characteristics such as medical/non-medical background, and the years of teaching were correlated with categories of learning issues identified. Students identify part of the faculty-set learning objectives during tutorials with a faculty tutor acting as a facilitator. Students' gender influences types of learning issues identified. Content expertise of tutors does not influence identification of learning needs by students.

Multi-Scale and Object-Oriented Analysis for Mountain Terrain Segmentation and Geomorphological Assessment

NASA Astrophysics Data System (ADS)

Marston, B. K.; Bishop, M. P.; Shroder, J. F.

2009-12-01

Digital terrain analysis of mountain topography is widely utilized for mapping landforms, assessing the role of surface processes in landscape evolution, and estimating the spatial variation of erosion. Numerous geomorphometry techniques exist to characterize terrain surface parameters, although their utility to characterize the spatial hierarchical structure of the topography and permit an assessment of the erosion/tectonic impact on the landscape is very limited due to scale and data integration issues. To address this problem, we apply scale-dependent geomorphometric and object-oriented analyses to characterize the hierarchical spatial structure of mountain topography. Specifically, we utilized a high resolution digital elevation model to characterize complex topography in the Shimshal Valley in the Western Himalaya of Pakistan. To accomplish this, we generate terrain objects (geomorphological features and landform) including valley floors and walls, drainage basins, drainage network, ridge network, slope facets, and elemental forms based upon curvature. Object-oriented analysis was used to characterize object properties accounting for object size, shape, and morphometry. The spatial overlay and integration of terrain objects at various scales defines the nature of the hierarchical organization. Our results indicate that variations in the spatial complexity of the terrain hierarchical organization is related to the spatio-temporal influence of surface processes and landscape evolution dynamics. Terrain segmentation and the integration of multi-scale terrain information permits further assessment of process domains and erosion, tectonic impact potential, and natural hazard potential. We demonstrate this with landform mapping and geomorphological assessment examples.

Vadose zone transport field study: Detailed test plan for simulated leak tests

DOE Office of Scientific and Technical Information (OSTI.GOV)

AL Ward; GW Gee

2000-06-23

The US Department of Energy (DOE) Groundwater/Vadose Zone Integration Project Science and Technology initiative was created in FY 1999 to reduce the uncertainty associated with vadose zone transport processes beneath waste sites at DOE's Hanford Site near Richland, Washington. This information is needed not only to evaluate the risks from transport, but also to support the adoption of measures for minimizing impacts to the groundwater and surrounding environment. The principal uncertainties in vadose zone transport are the current distribution of source contaminants and the natural heterogeneity of the soil in which the contaminants reside. Oversimplified conceptual models resulting from thesemore » uncertainties and limited use of hydrologic characterization and monitoring technologies have hampered the understanding contaminant migration through Hanford's vadose zone. Essential prerequisites for reducing vadose transport uncertainly include the development of accurate conceptual models and the development or adoption of monitoring techniques capable of delineating the current distributions of source contaminants and characterizing natural site heterogeneity. The Vadose Zone Transport Field Study (VZTFS) was conceived as part of the initiative to address the major uncertainties confronting vadose zone fate and transport predictions at the Hanford Site and to overcome the limitations of previous characterization attempts. Pacific Northwest National Laboratory (PNNL) is managing the VZTFS for DOE. The VZTFS will conduct field investigations that will improve the understanding of field-scale transport and lead to the development or identification of efficient and cost-effective characterization methods. Ideally, these methods will capture the extent of contaminant plumes using existing infrastructure (i.e., more than 1,300 steel-cased boreholes). The objectives of the VZTFS are to conduct controlled transport experiments at well-instrumented field sites at Hanford to: identify mechanisms controlling transport processes in soils typical of the hydrogeologic conditions of Hanford's waste disposal sites; reduce uncertainty in conceptual models; develop a detailed and accurate database of hydraulic and transport parameters for validation of three-dimensional numerical models; identify and evaluate advanced, cost-effective characterization methods with the potential to assess changing conditions in the vadose zone, particularly as surrogates of currently undetectable high-risk contaminants. This plan provides details for conducting field tests during FY 2000 to accomplish these objectives. Details of additional testing during FY 2001 and FY 2002 will be developed as part of the work planning process implemented by the Integration Project.« less

Relationship between daily affect and overeating-only, loss of control eating-only, and binge eating episodes in obese adults

PubMed Central

Berg, Kelly C.; Peterson, Carol B.; Crosby, Ross D.; Cao, Li; Crow, Scott J.; Engel, Scott G.; Wonderlich, Stephen A.

2013-01-01

The two objectives of the current study were: (1) to identify daily patterns of negative affect (NA) in obese individuals; and (2) to determine whether daily affect patterns were related to overeating without loss of control (OE-only), loss of control eating without overeating (LOC-only), and binge eating (BE) episodes. Fifty obese (BMI=40.3±08.5) adults (84.0% female) completed a two-week ecological momentary assessment protocol during which they completed assessments of NA and indicated whether their eating episodes were characterized by OE and/or LOC. Latent growth mixture modeling (LGMM) was used to identify daily trajectories of NA. GEE analysis was used to determine whether daily affect trajectories were differentially related to the frequency of OE-only, LOC-only, and BE episodes. The LGMM analyses identified nine unique trajectories of NA. Significantly higher frequencies of OE-only and BE episodes occurred on days characterized by high or increasing levels of NA. There were no significant differences between classes for the frequency of LOC-only episodes. These data suggest that NA may act as an antecedent to OE-only and BE episodes and that targeting “problematic affect days” may reduce the occurrence of OE-only and BE episodes among obese individuals. PMID:24200217

AACP Strategy for Addressing the Professional Development Needs of Department Chairs

PubMed Central

Rodriguez, Tobias E.; Weinstein, George; Sorofman, Bernard A.; Bosso, John A.; Kerr, Robert A.; Haden, N. Karl

2012-01-01

Objectives. Characterize the skills and abilities required for department chairs, identify development needs, and then create AACP professional development programs for chairs. Methods. A 30-question electronic survey was sent to AACP member department chairs related to aspects of chairing an academic department. Results. The survey identified development needs in the leadership, management, and personal abilities required for effective performance as department chair. The information was used to prioritize topics for subsequent AACP development programs. Subsequent programs conducted at AACP Interim and Annual Meetings were well attended and generally received favorable reviews from participants. A list of development resources was placed on the AACP website. Conclusions. This ongoing initiative is part of an AACP strategy to identify and address the professional development needs of department chairs. Survey results may also inform faculty members and other academic leaders about the roles and responsibilities of department chairs. PMID:22919099

Sleep, Dietary, and Exercise Behavioral Clusters Among Truck Drivers With Obesity: Implications for Interventions.

PubMed

Olson, Ryan; Thompson, Sharon V; Wipfli, Brad; Hanson, Ginger; Elliot, Diane L; Anger, W Kent; Bodner, Todd; Hammer, Leslie B; Hohn, Elliot; Perrin, Nancy A

2016-03-01

The objectives of the study were to describe a sample of truck drivers, identify clusters of drivers with similar patterns in behaviors affecting energy balance (sleep, diet, and exercise), and test for cluster differences in health safety, and psychosocial factors. Participants' (n = 452, body mass index M = 37.2, 86.4% male) self-reported behaviors were dichotomized prior to hierarchical cluster analysis, which identified groups with similar behavior covariation. Cluster differences were tested with generalized estimating equations. Five behavioral clusters were identified that differed significantly in age, smoking status, diabetes prevalence, lost work days, stress, and social support, but not in body mass index. Cluster 2, characterized by the best sleep quality, had significantly lower lost workdays and stress than other clusters. Weight management interventions for drivers should explicitly address sleep, and may be maximally effective after establishing socially supportive work environments that reduce stress exposures.

Ovary and vaginal epithelium dynamics during the estrous cycle in Dasyprocta prymnolopha Wagler, 1831: ultrasound and cytological examinations

PubMed Central

Carreiro, Artur N.; Diniz, João A. R. A.; Souza, Joyce G.; Araújo, Débora V. F.; Dias, Rômulo F. F.; Azerêdo, Liliane M. S.; Rocha, Ediane F.; La Salles, Ana Y. F.; Peña-Alfaro, Carlos E.; Carvalho, Maria A. M.; Illera, Maria J.

2018-01-01

The objective of the present study was to monitor and characterize morphological alterations in ovaries of agouti (Dasyprocta prymnolopha), reared in captivity, by using abdominal ultrasonography. All animals underwent daily vaginal cytological examination to identify the current cycle phase. For each phase of the estrous cycle, ultrasound examinations were carried out to identify and describe the morphology of both ovaries. Topographic parameters in an ultrasound window were established to locate the ovaries. The agouti estrous cycle lasted an average of 29.94 ± 6.77 days. During vaginal cytology examinations, all cell types were identified, and each phase of the estrous cycle was established by cell counts. No significant alterations were observed in the assessed ovarian morphometry measurements. In 75% of the animals examined, ovarian follicle presence was observed in the proestrus phase. PMID:29284211

First-time observation of Mastro Giorgio masterpieces by means of non-destructive techniques

NASA Astrophysics Data System (ADS)

Padeletti, G.; Ingo, G. M.; Bouquillon, A.; Pages-Camagna, S.; Aucouturier, M.; Roehrs, S.; Fermo, P.

2006-06-01

For the first time some excellent pieces belonging to the majolica production of the great master Giorgio Andreoli from Gubbio (Central Italy) have been characterized from a chemical and structural point of view with the aim to identify the composition of both pigments and lustres. A series of particle-induced X-ray emission (PIXE), Rutherford backscattering spectrometry (RBS) and Raman analyses have been performed on some plates coming from Museo del Palazzo dei Consoli (Gubbio) and several French museums (Louvre, Musée National de la Céramique, Musée National de la Renaissance) lustred by Giorgio Andreoli and decorated by famous majolica painters such as Francesco Xanto Avelli. The three techniques are complementary and useful in the investigation of art objects since they are non-destructive. Furthermore, the low detection limits allow the identification of all elements and compounds present, and RBS allows concentration profiling, too. It is worth noticing that the examined objects are characterized by the presence of both gold and ruby-red lustres, a peculiarity of Mastro Giorgio’s technique. The measurements by PIXE and RBS have been carried out on the AGLAE accelerator at C2RMF, Louvre Palace.

Can CanMEDS competencies be developed in medical school anatomy laboratories? A literature review.

PubMed

Hefler, Joshua; Ramnanan, Christopher J

2017-06-16

The purpose of this literature review was to identify potential ways in which undergraduate medical anatomy education may be relevant to the CanMEDS Roles, a competency-based framework used throughout Canadian medical training. A scoping review of medical education literature was conducted in March 2017 for English language publications that included key words related to anatomy education and to key competencies formally described for each of the Roles in the CanMEDS 2015 framework. Indicated benefits were then collated, characterized, and synthesized for each CanMEDS Role. There were 71 studies identified describing original findings. Perceived benefits of anatomy education were most often identified for competencies related to the Medical Expert Role. Multiple studies also cited benefits related to the Scholar, Professional and Collaborator Roles. There was a lack of literature related to the Health Advocate, Communicator, and Leader Roles. The majority of benefits defined in the literature were limited to student perceptions rather than objectively measured outcomes. There is some evidence to suggest that anatomy education can facilitate the development of core competencies related to several CanMEDS Roles, outside of simply developing medical knowledge in the Medical Expert Role. Future studies need to develop methods to objectively assess outcomes related to these competencies.

The Human Microbiome and Understanding the 16S rRNA Gene in Translational Nursing Science

PubMed Central

Ames, Nancy J.; Ranucci, Alexandra; Moriyama, Brad; Wallen, Gwenyth R.

2017-01-01

Background As more is understood regarding the human microbiome, it is increasingly important for nurse scientists and health care practitioners to analyze these microbial communities and their role in health and disease.16S rRNA sequencing is a key methodology in identifying these bacterial populations that has recently transitioned from use primarily in research to having increased utility in clinical settings. Objectives The objectives of this review are to: (a) describe 16S rRNA sequencing and its role in answering research questions important to nursing science; (b) provide an overview of the oral, lung and gut microbiomes and relevant research; and (c) identify future implications for microbiome research and 16S sequencing in translational nursing science. Discussion Sequencing using the 16S rRNA gene has revolutionized research and allowed scientists to easily and reliably characterize complex bacterial communities. This type of research has recently entered the clinical setting, one of the best examples involving the use of 16S sequencing to identify resistant pathogens, thereby improving the accuracy of bacterial identification in infection control. Clinical microbiota research and related requisite methods are of particular relevance to nurse scientists—individuals uniquely positioned to utilize these techniques in future studies in clinical settings. PMID:28252578

THE YOUNG STELLAR POPULATION OF LYNDS 1340. AN INFRARED VIEW

DOE Office of Scientific and Technical Information (OSTI.GOV)

Kun, M.; Moór, A.; Wolf-Chase, G.

We present results of an infrared study of the molecular cloud Lynds 1340, forming three groups of low- and intermediate-mass stars. Our goals are to identify and characterize the young stellar population of the cloud, study the relationships between the properties of the cloud and the emergent stellar groups, and integrate L1340 into the picture of the star-forming activity of our Galactic environment. We selected candidate young stellar objects (YSOs) from the Spitzer and WISE databases using various published color criteria and classified them based on the slope of the spectral energy distribution (SED). We identified 170 Class II, 27more » flat SED, and 45 Class 0/I sources. High angular resolution near-infrared observations of the RNO 7 cluster, embedded in L1340, revealed eight new young stars of near-infrared excess. The surface density distribution of YSOs shows three groups, associated with the three major molecular clumps of L1340, each consisting of ≲100 members, including both pre-main-sequence stars and embedded protostars. New Herbig–Haro objects were identified in the Spitzer images. Our results demonstrate that L1340 is a prolific star-forming region of our Galactic environment in which several specific properties of the intermediate-mass mode of star formation can be studied in detail.« less

The hierarchical brain network for face recognition.

PubMed

Zhen, Zonglei; Fang, Huizhen; Liu, Jia

2013-01-01

Numerous functional magnetic resonance imaging (fMRI) studies have identified multiple cortical regions that are involved in face processing in the human brain. However, few studies have characterized the face-processing network as a functioning whole. In this study, we used fMRI to identify face-selective regions in the entire brain and then explore the hierarchical structure of the face-processing network by analyzing functional connectivity among these regions. We identified twenty-five regions mainly in the occipital, temporal and frontal cortex that showed a reliable response selective to faces (versus objects) across participants and across scan sessions. Furthermore, these regions were clustered into three relatively independent sub-networks in a face-recognition task on the basis of the strength of functional connectivity among them. The functionality of the sub-networks likely corresponds to the recognition of individual identity, retrieval of semantic knowledge and representation of emotional information. Interestingly, when the task was switched to object recognition from face recognition, the functional connectivity between the inferior occipital gyrus and the rest of the face-selective regions were significantly reduced, suggesting that this region may serve as an entry node in the face-processing network. In sum, our study provides empirical evidence for cognitive and neural models of face recognition and helps elucidate the neural mechanisms underlying face recognition at the network level.

Autonomous Object Characterization with Large Datasets

DTIC Science & Technology

2015-10-18

desk, where a substantial amount of effort is required to transform raw photometry into a data product, minimizing the amount of time the analyst has...were used to explore concepts in satellite characterization and satellite state change. The first algorithm provides real- time stability estimation... Timely and effective space object (SO) characterization is a challenge, and requires advanced data processing techniques. Detection and identification

The Characterization of Deqi during Moxibustion in Stroke Rats

PubMed Central

Lv, Zhimai; Liu, Zhongyong; Huang, Dandan; Chen, Rixin; Xie, Dingyi

2013-01-01

The efficacy of acupuncture and moxibustion is closely related to Deqi phenomenons, which are some subjective feelings. However, no one has reported the objective characterization of Deqi. Our preliminary research has found a phenomenon of tail temperature increasing (TTI) obviously in some stroke rats by suspended moxibustion at the acupoint dà zhuī (DU 14), which is similar to one characterization of Deqi during moxibustion that moxibustion heat is transferred from the original moxibustion acupoint to the other areas of the body. We wonder whether TTI is the objective indicator of Deqi characterization in animals. The present study showed that the stroke rat's recovery was also associated with TTI phenomenon. This suggests that TTI phenomenon is one objective characterization of the Deqi in stroke rats. Application of the TTI phenomenon contributes to explore the physiological mechanism of Deqi. PMID:24194777

The Characterization of Deqi during Moxibustion in Stroke Rats.

PubMed

Lv, Zhimai; Liu, Zhongyong; Huang, Dandan; Chen, Rixin; Xie, Dingyi

2013-01-01

The efficacy of acupuncture and moxibustion is closely related to Deqi phenomenons, which are some subjective feelings. However, no one has reported the objective characterization of Deqi. Our preliminary research has found a phenomenon of tail temperature increasing (TTI) obviously in some stroke rats by suspended moxibustion at the acupoint dà zhuī (DU 14), which is similar to one characterization of Deqi during moxibustion that moxibustion heat is transferred from the original moxibustion acupoint to the other areas of the body. We wonder whether TTI is the objective indicator of Deqi characterization in animals. The present study showed that the stroke rat's recovery was also associated with TTI phenomenon. This suggests that TTI phenomenon is one objective characterization of the Deqi in stroke rats. Application of the TTI phenomenon contributes to explore the physiological mechanism of Deqi.

Bacteriocin from Bacillus subtilis as a novel drug against diabetic foot ulcer bacterial pathogens

PubMed Central

Joseph, Baby; Dhas, Berlina; Hena, Vimalin; Raj, Justin

2013-01-01

Objective To isolate and identify Bacillus subtilis (B. subtilis) from soil and to characterize and partially purify the bacteriocin. To evaluate the antimicrobial activity against four diabetic foot ulcer bacterial pathogens. Methods Genotypic identification was done based on Bergey's manual of systemic bacteriology. Antimicrobial susceptibility test was done by Kirby-Bauer disc diffusion method. Colonies were identified by colony morphology and biochemical characterization and also compared with MTCC 121 strain. Further identification was done by 16S rRNA sequencing. Inhibitory activities of partially purified bacteriocin on all the DFU isolates were done by agar well diffusion method. The strain was identified to produce bacteriocin by stab overlay assay. Bacteriocin was extracted by organic solvent extraction using chloroform, further purified by HPLC and physical, and chemical characterization was performed. Results The four isolates showed high level of resistance to amoxyclav and sensitivity to ciprofloxacin. HPLC purification revealed that the extracts are bacteriocin. The phylogenetic tree analysis results showed that the isolate was 99% related to B. subtilis BSF01. The results reveled activity to all the four isolates and high level of activity was seen in case of Klebsiella sp. Conclusions Partially purified bacteriocin was found to have antimicrobial activity against the four diabetic foot ulcer bacterial pathogens, which can thus be applied as a better drug molecule on further studies. The strain B. subtilis are found to be safe for use and these antimicrobial peptides can be used as an antimicrobial in humans to treat DFU bacterial pathogens. PMID:24093784

Purification and immunochemical characterization of Pla l 2, the profilin from Plantago lanceolata.

PubMed

Moya, Raquel; Rubio, Virginia; Beitia, Juan Mª; Carnés, Jerónimo; López-Matas, M Angeles

2017-03-01

Profilins are small actin-binding proteins found in eukaryotes and involved in cell development, cytokinesis, membrane trafficking, and cell motility. From an allergenic point of view, profilins are panallergens usually involved in allergic polysensitization, although they are generally recognized as minor allergens. The objectives of this study were to identify and characterize the profilin from Plantago lanceolata pollen and to investigate the cross-reactivity between profilins from different pollen allergenic sources. Profilins from P. lancelolata (Pla l 2) and palm tree pollen (Pho d 2) were purified by affinity chromatography, deeply characterized and identified by mass spectrometry. Pla l 2 allergenicity was confirmed by immunoblot with serum samples from a patient population sensitized to profilin. Immunoblot inhibition was performed to study IgG reactivity between different pollen profilins. IgE cross-reactivity was demonstrated by ImmunoCAP inhibition. Pla l 2 is the second P. lanceolata allergen included in the IUIS Allergen Nomenclature database. Four peptides from purified Pla l 2 were identified with percentages of homology with other pollen profilins between 73 and 86%. Eighty-six percent (21/24) of the patient population recognized Pla l 2. The allergenic relatedness between Pla l 2, Pho d 2 and six pollen profilins was confirmed, and IgE cross-reactivity of Pla l 2 with rBet v 2 and rPhl p 12 was demonstrated. Pla l 2 is the profilin from P. lanceolata. The demonstrated allergenicity of this protein and its cross-reactivity with other pollen profilins support its use in profilin diagnostic assays. Copyright © 2017 Elsevier Ltd. All rights reserved.

Cloning and characterization of an 11S legumin, Car i 4, a major allergen in pecan.

PubMed

Sharma, Girdhari M; Irsigler, Andre; Dhanarajan, Pushparani; Ayuso, Rosalia; Bardina, Luda; Sampson, Hugh A; Roux, Kenneth H; Sathe, Shridhar K

2011-09-14

Among tree nut allergens, pecan allergens remain to be identified and characterized. The objective was to demonstrate the IgE-binding ability of pecan 11S legumin and characterize its sequential IgE-binding epitopes. The 11S legumin gene was amplified from a pecan cDNA library and expressed as a fusion protein in Escherichia coli. The native 11S legumin in pecan extract was identified by mass spectrometry/mass spectrometry (MS/MS). Sequential epitopes were determined by probing the overlapping peptides with three serum pools prepared from different patients' sera. A three-dimensional model was generated using almond legumin as a template and compared with known sequential epitopes on other allergenic tree nut homologues. Of 28 patients tested by dot blot, 16 (57%) bound to 11S legumin, designated Car i 4. MS/MS sequencing of native 11S legumin identified 33 kDa acidic and 20-22 kDa basic subunits. Both pecan and walnut seed protein extracts inhibited IgE binding to recombinant Car i 4, suggesting cross-reactivity with Jug r 4. Sequential epitope mapping results of Car i 4 revealed weak, moderate, and strong reactivity of serum pools against 10, 5, and 4 peptides, respectively. Seven peptides were recognized by all three serum pools, of which two were strongly reactive. The strongly reactive peptides were located in three discrete regions of the Car i 4 acidic subunit sequence (residues 118-132, 208-219, and 238-249). Homology modeling of Car i 4 revealed significant overlapping regions shared in common with other tree nut legumins.

Characterizing Sleep Structure Using the Hypnogram

PubMed Central

Swihart, Bruce J.; Caffo, Brian; Bandeen-Roche, Karen; Punjabi, Naresh M.

2008-01-01

Objectives: Research on the effects of sleep-disordered breathing (SDB) on sleep structure has traditionally been based on composite sleep-stage summaries. The primary objective of this investigation was to demonstrate the utility of log-linear and multistate analysis of the sleep hypnogram in evaluating differences in nocturnal sleep structure in subjects with and without SDB. Methods: A community-based sample of middle-aged and older adults with and without SDB matched on age, sex, race, and body mass index was identified from the Sleep Heart Health Study. Sleep was assessed with home polysomnography and categorized into rapid eye movement (REM) and non-REM (NREM) sleep. Log-linear and multistate survival analysis models were used to quantify the frequency and hazard rates of transitioning, respectively, between wakefulness, NREM sleep, and REM sleep. Results: Whereas composite sleep-stage summaries were similar between the two groups, subjects with SDB had higher frequencies and hazard rates for transitioning between the three states. Specifically, log-linear models showed that subjects with SDB had more wake-to-NREM sleep and NREM sleep-to-wake transitions, compared with subjects without SDB. Multistate survival models revealed that subjects with SDB transitioned more quickly from wake-to-NREM sleep and NREM sleep-to-wake than did subjects without SDB. Conclusions: The description of sleep continuity with log-linear and multistate analysis of the sleep hypnogram suggests that such methods can identify differences in sleep structure that are not evident with conventional sleep-stage summaries. Detailed characterization of nocturnal sleep evolution with event history methods provides additional means for testing hypotheses on how specific conditions impact sleep continuity and whether sleep disruption is associated with adverse health outcomes. Citation: Swihart BJ; Caffo B; Bandeen-Roche K; Punjabi NM. Characterizing sleep structure using the hypnogram. J Clin Sleep Med 2008;4(4):349–355. PMID:18763427

Revealing mesoscopic structural universality with diffusion

PubMed Central

Novikov, Dmitry S.; Jensen, Jens H.; Helpern, Joseph A.; Fieremans, Els

2014-01-01

Measuring molecular diffusion is widely used for characterizing materials and living organisms noninvasively. This characterization relies on relations between macroscopic diffusion metrics and structure at the mesoscopic scale commensurate with the diffusion length. Establishing such relations remains a fundamental challenge, hindering progress in materials science, porous media, and biomedical imaging. Here we show that the dynamical exponent in the time dependence of the diffusion coefficient distinguishes between the universality classes of the mesoscopic structural complexity. Our approach enables the interpretation of diffusion measurements by objectively selecting and modeling the most relevant structural features. As an example, the specific values of the dynamical exponent allow us to identify the relevant mesoscopic structure affecting MRI-measured water diffusion in muscles and in brain, and to elucidate the structural changes behind the decrease of diffusion coefficient in ischemic stroke. PMID:24706873

DOE Office of Scientific and Technical Information (OSTI.GOV)

Bernstein, S.E.

Hereditary anemias of mice are the chief objects of investigation, specificially four macrocytic anemias, 3 types of hemolytic anemia, nonhemolytic microcytic anemia, transitory siderocytic anemia, sex-linked iron-transport anemia, the autoimmune hemolytic anemia of NZB mice, an ..cap alpha..-thalassemia and a new hypochromic anemia with hemochromatosis. New types of anemia may be analyzed as new mutations appear. Three new mutations have been identified during the past 18 months. These anemias are studied through characterization of peripheral blood values, determinations of radiosensitivity under a variety of conditions, measurements of iron metabolism and heme synthesis, study of normal and abnormal erythrocyte membrane proteins,more » histological and biochemical characterization of blood-forming tissue, functional tests of the stem-cell component, examination of responses to erythroid stimuli, and transplantation of tissue and parabiosis between individuals of differently affected genotypes. 31 refs.« less

Functional connectivity abnormalities and associated cognitive deficits in fetal alcohol Spectrum disorders (FASD).

PubMed

Wozniak, Jeffrey R; Mueller, Bryon A; Mattson, Sarah N; Coles, Claire D; Kable, Julie A; Jones, Kenneth L; Boys, Christopher J; Lim, Kelvin O; Riley, Edward P; Sowell, Elizabeth R

2017-10-01

Consistent with well-documented structural and microstructural abnormalities in prenatal alcohol exposure (PAE), recent studies suggest that functional connectivity (FC) may also be disrupted. We evaluated whole-brain FC in a large multi-site sample, examined its cognitive correlates, and explored its potential to objectively identify neurodevelopmental abnormality in individuals without definitive dysmorphic features. Included were 75 children with PAE and 68 controls from four sites. All participants had documented heavy prenatal alcohol exposure. All underwent a formal evaluation of physical anomalies and dysmorphic facial features. MRI data were collected using modified matched protocols on three platforms (Siemens, GE, and Philips). Resting-state FC was examined using whole-brain graph theory metrics to characterize each individual's connectivity. Although whole-brain FC metrics did not discriminate prenatally-exposed from unexposed overall, atypical FC (> 1 standard deviation from the grand mean) was significantly more common (2.7 times) in the PAE group vs. In a subset of 55 individuals (PAE and controls) whose dysmorphology examination could not definitively characterize them as either Fetal Alcohol Syndrome (FAS) or non-FAS, atypical FC was seen in 27 % of the PAE group, but 0 % of controls. Across participants, a 1 % difference in local network efficiency was associated with a 36 point difference in global cognitive functioning. Whole-brain FC metrics have potential to identify individuals with objective neurodevelopmental abnormalities from prenatal alcohol exposure. When applied to individuals unable to be classified as FAS or non-FAS from dysmorphology alone, these measures separate prenatally-exposed from non-exposed with high specificity.

[Molecular diagnosis of cystic fibrosis in 93 Argentinean patients and detection of heterozygotes in affected families. Impact on health services and therapeutic advances].

PubMed

Oller de Ramírez, Ana M; Ghio, Addy; Melano de Botelli, Myrna; Dodelson de Kremer, Raquel

2008-08-01

The cystic fibrosis is an autosomal recessive disease caused by more than 1500 mutations and variants in the cystic fibrosis transmembrane conductance regulator gene. To establish the spectrum and frequency of mutations on this gene in Argentinean patients.To detect heterozygotes in affected families. We investigated 91 clinical and biochemically confirmed patients with 2 elevated sweat tests and 2 sterile adults. We worked with 165 relatives. The molecular diagnosis was accomplished in 3 serial stages: a) determination of 29 frequent mutations; b) haplotypes for microsatellites; c) an extensive screening of gene through single strand conformation analysis and multiplex denaturing gradient gel electrophoresis with sequencing of abnormal patterns. Once patient's genotype was confirmed, we investigated the heterozygotes' state in the relatives. 1ST OBJECTIVE: Fourteen mutations were identified. Three more mutations were detected and other 11 mutations were characterized, 3 of them novel (p.G27R, c.622-2A>G, p.W277R). In total, we have identified 28 mutations responsible for 90.3% of the mutated alleles, 14 with a higher frequency than 1%. 2ND OBJECTIVE: From 165 investigated people, 143 were confirmed as heterozygotes and with normal genotype 22. This work contributed to the molecular characterization of patients with classic and atypical phenotypes and to the detection of great numbers of carriers. New pharmacological therapeutic investigations are based on the mutation type. Therefore, knowledge of patients, mutations (genotype) has significant importance for the future application of specific therapies.

Automatic labeling and characterization of objects using artificial neural networks

NASA Technical Reports Server (NTRS)

Campbell, William J.; Hill, Scott E.; Cromp, Robert F.

1989-01-01

Existing NASA supported scientific data bases are usually developed, managed and populated in a tedious, error prone and self-limiting way in terms of what can be described in a relational Data Base Management System (DBMS). The next generation Earth remote sensing platforms, i.e., Earth Observation System, (EOS), will be capable of generating data at a rate of over 300 Mbs per second from a suite of instruments designed for different applications. What is needed is an innovative approach that creates object-oriented databases that segment, characterize, catalog and are manageable in a domain-specific context and whose contents are available interactively and in near-real-time to the user community. Described here is work in progress that utilizes an artificial neural net approach to characterize satellite imagery of undefined objects into high-level data objects. The characterized data is then dynamically allocated to an object-oriented data base where it can be reviewed and assessed by a user. The definition, development, and evolution of the overall data system model are steps in the creation of an application-driven knowledge-based scientific information system.

On the Dielectric Properties of the Martian-like Surface Sediments

NASA Technical Reports Server (NTRS)

Heggy, E.; Clifford, S. M.; Morris, R. V.; Paillou, P.; Ruffie, G.

2004-01-01

We have undertaken laboratory electromagnetic characterization of the total set of minerals identified by TES on the Martian surface in order to investigate experimentally the dielectric properties of the sediments covering it in the frequency range from 1 to 30 MHz. Volcanic Rocks with a well defined mineralogy and petrology from potential terrestrial analogues sites have also been included in the study. Our primary objective is to evaluate the range of electrical and magnetic losses that may be encountered by the various Radar sounding and imaging experiments dedicated to map the Martian subsurface searching for underground water. The electromagnetic properties of these Mars-like materials will be presented as a function of various geophysical parameters, such as porosity, bulk density and temperature. The secondary objective, is to locate regions were surface dielectric conditions are suitable for subsurface sounding.

Technology evaluation of characterization of the air void system in concrete.

DOT National Transportation Integrated Search

2009-09-01

The objective of this project was to evaluate current technologies that have the capability of characterizing the air void system in concrete within the first several hours of placement. This objective was met by developing a comprehensive technology...

Technology evaluation on characterization of the air void system in concrete.

DOT National Transportation Integrated Search

2009-09-17

The objective of this project was to evaluate current technologies that have the capability of characterizing the air void system in concrete within the first several hours of placement. This objective was met by developing a comprehensive technology...

Surface characterization of pretreated and microbial-treated populus cross-sections

DOE Office of Scientific and Technical Information (OSTI.GOV)

Tolbert, Allison K.

The first objective of this thesis is to illustrate the advantages of surface characterization in biomass utilization studies. The second objective is to gain insight into the workings of potential consolidated bioprocessing microorganisms on the surface of poplar samples. The third objective is to determine the impact biomass recalcitrance has on enzymatic hydrolysis and microbial fermentation in relation to the surface chemistry.

A spatial model of potential jaguar habitat in Arizona

USGS Publications Warehouse

Hatten, J.R.; Averill-Murray, A.; van Pelt, W.E.

2005-01-01

The jaguar (Panthera onca) is an endangered species that occasionally visits the southwestern United States from Mexico. The number of jaguar sightings per decade has declined over the last 100 years in Arizona, USA, raising conservation concerns for the species at a local and national level. In 1997, state, federal, and local governments with land-management responsibilities agreed to characterize and identify potential jaguar habitat in Arizona and New Mexico. Specifically, the objectives of our analysis were 2-fold: (1) characterize potential jaguar habitat in Arizona from historic sighting records and (2) create a statewide habitat suitability map. We used a Geographic Information System (GIS) to characterize potential jaguar habitat by overlaying historic jaguar sightings (25) on landscape and habitat features believed important (e.g., vegetation biomes and series, elevation, terrain ruggedness, proximity to perennial or intermittent water sources, human density). The amount of Arizona (%) identified as potential jaguar habitat ranged from 21% to 30% depending on the input variables. Most jaguar sightings were in scrub grasslands between 1,220 and 1,829-m elevation in southeastern Arizona, in intermediately to extremely rugged terrain, and within 10 km of a water source. Conservation efforts should focus on protecting the most suitable jaguar habitat in southeastern Arizona (i.e., Santa Cruz, Pima, Cochise, Pinal, Graham counties), travel corridors within and outside Arizona, and jaguar habitat in the Sierra Madres of Sonora, Mexico.

Molecular genotyping of ABO blood groups in some population groups from India.

PubMed

Ray, Sabita; Gorakshakar, Ajit C; Vasantha, K; Nadkarni, Anita; Italia, Yazdi; Ghosh, Kanjaksha

2014-01-01

Indian population is characterized by the presence of various castes and tribal groups. Various genetic polymorphisms have been used to differentiate among these groups. Amongst these, the ABO blood group system has been extensively studied. There is no information on molecular genotyping of ABO blood groups from India. Therefore, the main objective of this study was to characterize the common A, B and O alleles by molecular analysis in some Indian population groups. One hundred samples from the mixed population from Mumbai, 101 samples from the Dhodia tribe and 100 samples from the Parsi community were included in this study. Initially, the samples were phenotyped by standard serologic techniques. PCR followed by single strand conformational polymorphsim (SSCP) was used for molecular ABO genotyping. Samples showing atypical SSCP patterns were further analysed by DNA sequencing to characterize rare alleles. Seven common ABO alleles with 19 different genotypes were found in the mixed population. The Dhodias showed 12 different ABO genotypes and the Parsis revealed 15 different ABO genotypes with six common ABO alleles identified in each of them. Two rare alleles were also identified. This study reports the distribution of molecular genotypes of ABO alleles among some population groups from India. Considering the extremely heterogeneous nature of the Indian population, in terms of various genotype markers like blood groups, red cell enzymes, etc., many more ABO alleles are likely to be encountered.

Evidence Report: Risk of Performance Errors Due to Training Deficiencies

NASA Technical Reports Server (NTRS)

Barshi, Immanuel

2012-01-01

The Risk of Performance Errors Due to Training Deficiencies is identified by the National Aeronautics and Space Administration (NASA) Human Research Program (HRP) as a recognized risk to human health and performance in space. The HRP Program Requirements Document (PRD) defines these risks. This Evidence Report provides a summary of the evidence that has been used to identify and characterize this risk. Given that training content, timing, intervals, and delivery methods must support crew task performance, and given that training paradigms will be different for long-duration missions with increased crew autonomy, there is a risk that operators will lack the skills or knowledge necessary to complete critical tasks, resulting in flight and ground crew errors and inefficiencies, failed mission and program objectives, and an increase in crew injuries.

Stratification and mobility in contemporary Egypt.

PubMed

Nagi, Saad Z; Nagi, Omar

2011-01-01

The objectives in this statement are to characterize and explain the patterns of change in stratification and mobility in Egypt, over the last half century, by placing them within conceptual, explanatory, and historical contexts. First, literature relevant to the primary concepts of "class" and "status", is reviewed. Second, four institutions whose influence is fundamental in shaping these patterns are identified to form an explanatory context: family, polity, economy, and education. And third, an historical account is presented to demonstrate the interplay of these institutions and their consequences for stratification and mobility. For this, four periods are identified that are marked by change in the dominance of institutions and their corresponding influence on stratification and mobility. In addition to data available in relevant literature, this analysis utilizes primary data generated through a national probability household survey.

A fractured rock geophysical toolbox method selection tool

USGS Publications Warehouse

Day-Lewis, F. D.; Johnson, C.D.; Slater, L.D.; Robinson, J.L.; Williams, J.H.; Boyden, C.L.; Werkema, D.D.; Lane, J.W.

2016-01-01

Geophysical technologies have the potential to improve site characterization and monitoring in fractured rock, but the appropriate and effective application of geophysics at a particular site strongly depends on project goals (e.g., identifying discrete fractures) and site characteristics (e.g., lithology). No method works at every site or for every goal. New approaches are needed to identify a set of geophysical methods appropriate to specific project goals and site conditions while considering budget constraints. To this end, we present the Excel-based Fractured-Rock Geophysical Toolbox Method Selection Tool (FRGT-MST). We envision the FRGT-MST (1) equipping remediation professionals with a tool to understand what is likely to be realistic and cost-effective when contracting geophysical services, and (2) reducing applications of geophysics with unrealistic objectives or where methods are likely to fail.

Neural network identification of aircraft nonlinear aerodynamic characteristics

NASA Astrophysics Data System (ADS)

Egorchev, M. V.; Tiumentsev, Yu V.

2018-02-01

The simulation problem for the controlled aircraft motion is considered in the case of imperfect knowledge of the modeling object and its operating conditions. The work aims to develop a class of modular semi-empirical dynamic models that combine the capabilities of theoretical and neural network modeling. We consider the use of semi-empirical neural network models for solving the problem of identifying aerodynamic characteristics of an aircraft. We also discuss the formation problem for a representative set of data characterizing the behavior of a simulated dynamic system, which is one of the critical tasks in the synthesis of ANN-models. The effectiveness of the proposed approach is demonstrated using a simulation example of the aircraft angular motion and identifying the corresponding coefficients of aerodynamic forces and moments.

Characterization of Nitrogen use efficiency in sweet sorghum

DOE Office of Scientific and Technical Information (OSTI.GOV)

Dweikat, Ismail; Clemente, Thomas

2014-09-09

Sweet sorghum (Sorghum bicolor L. Moench) has the potential to augment the increasing demand for alternative fuels and for the production of input efficient, environmentally friendly bioenergy crops. Nitrogen (N) and water availability are considered two of the major limiting factors in crop growth. Nitrogen fertilization accounts for about 40% of the total production cost in sorghum. In cereals, including sorghum, the nitrogen use efficiency (NUE) from fertilizer is approximately 33% of the amount applied. There is therefore extensive concern in relation to the N that is not used by the plant, which is lost by leaching of nitrate, denitrificationmore » from the soil, and loss of ammonia to the atmosphere, all of which can have deleterious environmental effects. To improve the potential of sweet sorghum as a leading and cost effective bioenergy crop, the enhancement of NUE must be addressed. To this end, we have identified a sorghum line (SanChi San) that displays about 25% increase in NUE over other sorghum lines. As such, the overarching goal of this project is to employ three complementary strategies to enhance the ability of sweet sorghum to become an efficient nitrogen user. To achieve the project goal, we will pursue the following specific objectives: Objective 1: Phenotypic characterization of SanChi San/Ck60 RILs under low and moderate N-availability including biochemical profiles, vegetative growth and seed yield Objective 2: Conduct quantitative trait loci (QTL) analysis and marker identification for nitrogen use efficiency (NUE) in a grain sorghum RIL population. Objective 3: Identify novel candidate genes for NUE using proteomic and gene expression profiling comparisons of high- and low-NUE RILs. Candidate genes will be brought into the pipeline for transgenic manipulation of NUE This project will apply the latest genomics resources to discover genes controlling NUE, one of the most complex and economically important traits in cereal crops. As a result of the completion of the proposed work, we will have: 1) identified novel alleles in wild sorghum germplasm that is useful to improve both cultivated grain and sweet sorghum; 2) been able to select individuals plants that exhibit high NUE within a breeding population on the basis of these markers; 3) acquired essential information necessary to examine the roles of GS and GOGAT, AlaT, along with impact of transcription factor Dof1, on N assimilation in sweet sorghum; and 4) The information learned will provide new opportunities for improving NUE in sorghum and other cereals.« less

Efficient characterization of phase space mapping in axially symmetric optical systems

NASA Astrophysics Data System (ADS)

Barbero, Sergio; Portilla, Javier

2018-01-01

Phase space mapping, typically between an object and image plane, characterizes an optical system within a geometrical optics framework. We propose a novel conceptual frame to characterize the phase mapping in axially symmetric optical systems for arbitrary object locations, not restricted to a specific object plane. The idea is based on decomposing the phase mapping into a set of bivariate equations corresponding to different values of the radial coordinate on a specific object surface (most likely the entrance pupil). These equations are then approximated through bivariate Chebyshev interpolation at Chebyshev nodes, which guarantees uniform convergence. Additionally, we propose the use of a new concept (effective object phase space), defined as the set of points of the phase space at the first optical element (typically the entrance pupil) that are effectively mapped onto the image surface. The effective object phase space provides, by means of an inclusion test, a way to avoid tracing rays that do not reach the image surface.

Metrological characterization of 3D imaging devices

NASA Astrophysics Data System (ADS)

Guidi, G.

2013-04-01

Manufacturers often express the performance of a 3D imaging device in various non-uniform ways for the lack of internationally recognized standard requirements for metrological parameters able to identify the capability of capturing a real scene. For this reason several national and international organizations in the last ten years have been developing protocols for verifying such performance. Ranging from VDI/VDE 2634, published by the Association of German Engineers and oriented to the world of mechanical 3D measurements (triangulation-based devices), to the ASTM technical committee E57, working also on laser systems based on direct range detection (TOF, Phase Shift, FM-CW, flash LADAR), this paper shows the state of the art about the characterization of active range devices, with special emphasis on measurement uncertainty, accuracy and resolution. Most of these protocols are based on special objects whose shape and size are certified with a known level of accuracy. By capturing the 3D shape of such objects with a range device, a comparison between the measured points and the theoretical shape they should represent is possible. The actual deviations can be directly analyzed or some derived parameters can be obtained (e.g. angles between planes, distances between barycenters of spheres rigidly connected, frequency domain parameters, etc.). This paper shows theoretical aspects and experimental results of some novel characterization methods applied to different categories of active 3D imaging devices based on both principles of triangulation and direct range detection.

A framework to spatially cluster air pollution monitoring sites in US based on the PM2.5 composition

PubMed Central

Austin, Elena; Coull, Brent A.; Zanobetti, Antonella; Koutrakis, Petros

2013-01-01

Background Heterogeneity in the response to PM2.5 is hypothesized to be related to differences in particle composition across monitoring sites which reflect differences in source types as well as climatic and topographic conditions impacting different geographic locations. Identifying spatial patterns in particle composition is a multivariate problem that requires novel methodologies. Objectives Use cluster analysis methods to identify spatial patterns in PM2.5 composition. Verify that the resulting clusters are distinct and informative. Methods 109 monitoring sites with 75% reported speciation data during the period 2003–2008 were selected. These sites were categorized based on their average PM2.5 composition over the study period using k-means cluster analysis. The obtained clusters were validated and characterized based on their physico-chemical characteristics, geographic locations, emissions profiles, population density and proximity to major emission sources. Results Overall 31 clusters were identified. These include 21 clusters with 2 or more sites which were further grouped into 4 main types using hierarchical clustering. The resulting groupings are chemically meaningful and represent broad differences in emissions. The remaining clusters, encompassing single sites, were characterized based on their particle composition and geographic location. Conclusions The framework presented here provides a novel tool which can be used to identify and further classify sites based on their PM2.5 composition. The solution presented is fairly robust and yielded groupings that were meaningful in the context of air-pollution research. PMID:23850585

A structure-based approach for colon gland segmentation in digital pathology

NASA Astrophysics Data System (ADS)

Ben Cheikh, Bassem; Bertheau, Philippe; Racoceanu, Daniel

2016-03-01

The morphology of intestinal glands is an important and significant indicator of the level of the severity of an inflammatory bowel disease, and has also been used routinely by pathologists to evaluate the malignancy and the prognosis of colorectal cancers such as adenocarcinomas. The extraction of meaningful information describing the morphology of glands relies on an accurate segmentation method. In this work, we propose a novel technique based on mathematical morphology that characterizes the spatial positioning of nuclei for intestinal gland segmentation in histopathological images. According to their appearance, glands can be divided into two types: hallow glands and solid glands. Hallow glands are composed of lumen and/or goblet cells cytoplasm, or filled with abscess in some advanced stages of the disease, while solid glands are composed of bunches of cells clustered together and can also be filled with necrotic debris. Given this scheme, an efficient characterization of the spatial distribution of cells is sufficient to carry out the segmentation. In this approach, hallow glands are first identified as regions empty of nuclei and surrounded by thick layers of epithelial cells, then solid glands are identified by detecting regions crowded of nuclei. First, cell nuclei are identified by color classification. Then, morphological maps are generated by the mean of advanced morphological operators applied to nuclei objects in order to interpret their spatial distribution and properties to identify candidates for glands central-regions and epithelial layers that are combined to extract the glandular structures.

Identification and characterization of genes related to cellulolytic activity in basidiomycetes.

PubMed

Volpini, A F N; Thomazine, T; Umeo, S H; Pereira, G A; Linde, G A; Valle, J S; Colauto, N B; Barcellos, F G; Souza, S G H

2016-09-16

Enzymes produced by basidiomycetes that are involved in the cellulose degradation process, and their respective codifying genes, must be identified to facilitate the development of novel biotechnological strategies and applications in the agro-industry. The objective of this study was to identify prospective cellulase-producing genes and characterize their cellulolytic activity, in order to elucidate the potential biotechnological applications (with respect to vegetal residues) of basidiomycetes. The basidiomycete strains Lentinula edodes U8-1, Lentinus crinitus U9-1, and Schizophyllum commune U6-7 were analyzed in this study. The cellulolytic activities of these fungi were evaluated based on the halo formation in carboxymethyl cellulose culture medium after dyeing with Congo red. The presence of cellulase-codifying genes (cel7A, cel6B, cel3A, and egl) in these fungal strains was also evaluated. L. edodes and S. commune presented the highest cellulolytic halo to mycelial growth radius ratio, followed by L. crinitus. Four genes were amplified in the L. edodes strain, whereas three and one genes were isolated from L. crinitus and S. commune, respectively. The cel6B gene (L. edodes) presented the conserved domain glyco_hydro_6 and characterized as cellobiohydrolase gene. The results of this study contribute to the existing knowledge on cellulases in basidiomycetes, and serve as a basis for future studies on the expression of these genes and the characterization of the catalytic activity of these enzymes. This allows for better utilization of these fungi in degrading vegetal fibers from agro-industrial residues and in other biotechnological applications.

Maintenance of coupling between stratosphere and troposphere annular modes in middle and late winter

NASA Astrophysics Data System (ADS)

de La Torre, L.; Gimeno, L.; Nieto, R.; Tesouro, M.; Añel, J. A.; Ribera, P.; García, R.; Hernández, E.

2003-04-01

The main objective of this work is to know when the coupling between stratosphere and troposphere Northern Annular Mode (NAM) is maintained during mid- and late winter (January-February-March) . Daily NAM-index time series calculated at 17 pressure levels from 1000 to 10-hPa for the period 1958-2001 were used in this study, as well as NCAR-NCEP reanalysis to characterize spatial patterns. So, a) we identified periods when coupling between stratospheric and tropospheric NAMs are intense/weak in mid-late winter, b) we tried to characterize these periods in terms of spatial patterns of geopotential anomalies and c) we characterized these periods in terms of stratospheric-tropospheric NAMs cross-correlation structures. Results suggest that: a)there is intense interannual variability in the number of uncoupling/coupling days, b) coupling periods are characterized by strong negative geopotential anomalies over Eurasia, c) there is only positive NAO values in the coupling days composite, c) there is two main pattens of cross-correlations, in one of them troposphere and stratosphere NAM are correlated at lag 0 and in the other troposphere NAM leads stratosphere NAM in about two weeks.

Genome-wide association study to identify potential genetic modifiers in a canine model for Duchenne muscular dystrophy.

PubMed

Brinkmeyer-Langford, Candice; Balog-Alvarez, Cynthia; Cai, James J; Davis, Brian W; Kornegay, Joe N

2016-08-22

Duchenne muscular dystrophy (DMD) causes progressive muscle degeneration, cardiomyopathy and respiratory failure in approximately 1/5,000 boys. Golden Retriever muscular dystrophy (GRMD) resembles DMD both clinically and pathologically. Like DMD, GRMD exhibits remarkable phenotypic variation among affected dogs, suggesting the influence of modifiers. Understanding the role(s) of genetic modifiers of GRMD may identify genes and pathways that also modify phenotypes in DMD and reveal novel therapies. Therefore, our objective in this study was to identify genetic modifiers that affect discrete GRMD phenotypes. We performed a linear mixed-model (LMM) analysis using 16 variably-affected dogs from our GRMD colony (8 dystrophic, 8 non-dystrophic). All of these dogs were either full or half-siblings, and phenotyped for 19 objective, quantitative biomarkers at ages 6 and 12 months. Each biomarker was individually assessed. Gene expression profiles of 59 possible candidate genes were generated for two muscle types: the cranial tibialis and medial head of the gastrocnemius. SNPs significantly associated with GRMD biomarkers were identified on multiple chromosomes (including the X chromosome). Gene expression levels for candidate genes located near these SNPs correlated with biomarker values, suggesting possible roles as GRMD modifiers. The results of this study enhance our understanding of GRMD pathology and represent a first step toward the characterization of GRMD modifiers that may be relevant to DMD pathology. Such modifiers are likely to be useful for DMD treatment development based on their relationships to GRMD phenotypes.

The future of planetary defense

NASA Astrophysics Data System (ADS)

Mainzer, A.

2017-04-01

Asteroids and comets have impacted Earth in the past and will do so in the future. While the frequency of impacts is reasonably well understood on geologic timescales, it is difficult to predict the next sizeable impact on human timescales by extrapolation from population statistics alone. Fortunately, by identifying and tracking individual objects, we can make precise predictions of any potential close encounters with Earth. As more advance notice is provided, the range of possible mitigation options expands. While the chance of an impact is very small, the potential consequences can be severe, meaning that sensible risk reduction measures should be undertaken. By implementing surveys, the risk of an unforeseen impact can be greatly reduced: the first step is finding the objects. Fortunately, the worldwide community of professional and amateur astronomers has made significant progress in discovering large near-Earth objects (NEOs). More than 95% of NEOs capable of causing global devastation (objects larger than 1 km in diameter) have been discovered, and none of these pose an impact hazard in the near future. Infrastructure is in place to link observations and compute close approaches in real time. Interagency and international collaborations have been undertaken to strengthen cooperative efforts to plan potential mitigation and civil defense campaigns. Yet much remains to be done. Approximately 70% of NEOs larger than 140 m (large enough to cause severe regional damage) remain undiscovered. With the existing surveys, it will take decades to identify the rest. Progress can be accelerated by undertaking new surveys with improved sensitivity.