NPOESS Preparatory Project Validation Program for Atmsophere Data Products from VIIRS

NASA Astrophysics Data System (ADS)

Starr, D.; Wong, E.

2009-12-01

The National Polar-orbiting Operational Environmental Satellite Suite (NPOESS) Program, in partnership with National Aeronautical Space Administration (NASA), will launch the NPOESS Preparatory Project (NPP), a risk reduction and data continuity mission, prior to the first operational NPOESS launch. The NPOESS Program, in partnership with Northrop Grumman Aerospace Systems (NGAS), will execute the NPP Validation program to ensure the data products comply with the requirements of the sponsoring agencies. Data from the NPP Visible/Infrared Imager/Radiometer Suite (VIIRS) will be used to produce Environmental Data Records (EDR's) for aerosol and clouds, specifically Aerosol Optical Thickness (AOT), Aerosol Particle Size Parameter (APSP), and Suspended Matter (SM); and Cloud Optical Thickness (COT), Cloud Effective Particle Size (CEPS), Cloud Top Temperature (CTT), Height (CTH) and Pressure (CTP), and Cloud Base Height (CBH). The Aerosol and Cloud EDR Validation Program is a multifaceted effort to characterize and validate these data products. The program involves systematic comparison to heritage data products, e.g., MODIS, and ground-based correlative data, such as AERONET and ARM data products, and potentially airborne field measurements. To the extent possible, the domain is global. The program leverages various investments that have and are continuing to be made by national funding agencies in such resources, as well as the operational user community and the broad Earth science user community. This presentation will provide an overview of the approaches, data and schedule for the validation of the NPP VIIRS Aerosol and Cloud environmental data products.

Navy Tactical Ocean Optical Products from the National Polar-orbiting Operational Environmental Satellite System (NPOESS)

NASA Astrophysics Data System (ADS)

Lyon, P. E.; Arnone, R.

2006-12-01

The Naval Research Laboratory at Stennis Space Center (NRLSSC) is preparing to produce optical products for Naval operations support from the National Polar-orbiting Operational Environmental Satellite System (NPOESS). This effort will leverage existing hardware and software systems in place at NRLSSC which are currently used to produce optical products from current sensors SeaWiFS and MODIS Aqua/Terra. This effort is part of an inter agency collaboration between NASA, NOAA, IPO, NRL and the prime contractor for NPOESS, Northrop Grumman / Raytheon. This poster presents an outline of the NRLSSC's plan for achieving the best possible optical products from NPOESS.

Mineralogical Study of Hydrated IDPs: X-Ray Diffraction and Transmission Electron Microscopy

NASA Technical Reports Server (NTRS)

Nakamura, K.; Keller, L. P.; Nakamura, T.; Noguchi, T.; Zolensky, M. E.

2004-01-01

Chondritic hydrated interplanetary dust particles (IDPs) comprise up to 50% of all IDPs collected in the stratosphere [1]. Hydrated IDPs are generally believed to be derived from asteroidal sources that have undergone aqueous alteration. However, the high C contents of hydrated IDPs (by 2 to 6X CI levels [2,3]) indicate that they are probably not derived from the same parent bodies sampled by the known chondritic meteorites. Some hydrated IDPs exhibit large deuterium enrichments [4] similar to those observed in anhydrous IDPs. Both anhydrous and hydrated IDPs contain a variety of anhydrous minerals such as silicates, sulfides, oxides, and carbonates. Controversies on hydrated IDPs still exist regarding their formation, history, and relationship to other primitive solar system materials, because of the lack of a systematic series of analysis on individual hydrated IDPs. In this study, we combine our observations of the bulk mineralogy, mineral/ organic chemistry in order to derive a more complete picture of hydrated IDPs.

Key Features of the National Polar-Orbiting Operational Environmental Satellite System (NPOESS) System Architecture

NASA Astrophysics Data System (ADS)

Pela, F.; Tsugawa, R. K.; Andreoli, L. J.

2004-12-01

The National Polar-Orbiting NPOESS, a tri-agency program, supports missions of the Department of Commerce (DOC)/National Oceanic and Atmospheric Administration (NOAA), the Department of Defense (DoD), and the National Aeronautics and Space Administration (NASA). NPOESS provides a critical, timely, reliable, and high quality space-based sensing capability to acquire and process global and regional environmental imagery and specialized meteorological, climatic, terrestrial, oceanographic, solar-geophysical, and other data products. These products are delivered to national weather and environmental facilities operated by NOAA and DoD, to NASA, and to environmental remote sensing science community users to support civil and military functions. These data are also provided in real time to field terminals deployed worldwide. The NPOESS architecture is built on a foundation of affordability, and the three pillars of data quality, latency, availability. Affordability refers to an over-arching awareness of cost to provide the best value to the government for implementing a converged system; some dimensions of cost include the cost for system development and implementation, the balance between development costs and operation and maintenance costs, and the fiscal year expenditure plans that meet schedule commitments. Data quality is characterized in terms of the attributes associated with Environmental Data Records (EDRs), and the products that are delivered to the four US Operational Centrals and field users. These EDRs are generated by the system using raw data from the space-borne sensors and spacecraft, in conjunction with science algorithms and calibration factors. Data latency refers to the time period between the detection of energy by a space-borne sensor to the delivery of a corresponding EDR. The system was designed to minimize data latency, and hence provide users with timely data. Availability refers to both data availability and system operational availability

Presolar Materials in a Giant Cluster IDP of Probable Cometary Origin

NASA Technical Reports Server (NTRS)

Messenger, S.; Brownlee, D. E.; Joswiak, D. J.; Nguyen, A. N.

2015-01-01

Chondritic porous interplanetary dust particles (CP-IDPs) have been linked to comets by their fragile structure, primitive mineralogy, dynamics, and abundant interstellar materials. But differences have emerged between 'cometary' CP-IDPs and comet 81P/Wild 2 Stardust Mission samples. Particles resembling Ca-Al-rich inclusions (CAIs), chondrules, and amoeboid olivine aggregates (AOAs) in Wild 2 samples are rare in CP-IDPs. Unlike IDPs, presolar materials are scarce in Wild 2 samples. These differences may be due to selection effects, such as destruction of fine grained (presolar) components during the 6 km/s aerogel impact collection of Wild 2 samples. Large refractory grains observed in Wild 2 samples are also unlikely to be found in most (less than 30 micrometers) IDPs. Presolar materials provide a measure of primitive-ness of meteorites and IDPs. Organic matter in IDPs and chondrites shows H and N isotopic anomalies attributed to low-T interstellar or protosolar disk chemistry, where the largest anomalies occur in the most primitive samples. Presolar silicates are abundant in meteorites with low levels of aqueous alteration (Acfer 094 approximately 200 ppm) and scarce in altered chondrites (e.g. Semarkona approximately 20 ppm). Presolar silicates in minimally altered CP-IDPs range from approximately 400 ppm to 15,000 ppm, possibly reflecting variable levels of destruction in the solar nebula or statistical variations due to small sample sizes. Here we present preliminary isotopic and mineralogical studies of a very large CP-IDP. The goals of this study are to more accurately determine the abundances of presolar components of CP-IDP material for comparison with comet Wild 2 samples and meteorites. The large mass of this IDP presents a unique opportunity to accurately determine the abundance of pre-solar grains in a likely cometary sample.

House Hearing Nunn-McCurdy Review of NPOESS

NASA Image and Video Library

2006-06-08

NASA Administrator Michael Griffin, testifies before the House Committee on Science and Technology regarding the future of the National Polar-orbiting Operational Environmental Satellite System (NPOESS) and results of the Nunn-McCurdy review of NOAA’s weather satellite program, Thursday, June 8, 2006, at the Rayburn House Office Building in Washington. Photo Credit: (NASA/Bill Ingalls)

House Hearing Nunn-McCurdy Review of NPOESS

NASA Image and Video Library

2006-06-08

Under Secretary of the Air Force Dr. Ronald Sega, testifies before the House Committee on Science and Technology regarding the future of the National Polar-orbiting Operational Environmental Satellite System (NPOESS) and results of the Nunn-McCurdy review of NOAA’s weather satellite program, Thursday, June 8, 2006, at the Rayburn House Office Building in Washington. Photo Credit: (NASA/Bill Ingalls)

House Hearing Nunn-McCurdy Review of NPOESS

NASA Image and Video Library

2006-06-07

Under Secretary of the Air Force Dr. Ronald Sega, testifies before the House Committee on Science and Technology regarding the future of the National Polar-orbiting Operational Environmental Satellite System (NPOESS) and results of the Nunn-McCurdy review of NOAA’s weather satellite program, Thursday, June 8, 2006, at the Rayburn House Office Building in Washington. Photo Credit: (NASA/Bill Ingalls)

House Hearing Nunn-McCurdy Review of NPOESS

NASA Image and Video Library

2006-06-07

NOAA Administrator, Vice Admiral Conrad C. Lautenbacher, Jr., U.S. Navy (Ret.), testifies before the House Committee on Science and Technology regarding the future of the National Polar-orbiting Operational Environmental Satellite System (NPOESS) and results of the Nunn-McCurdy review of NOAA’s weather satellite program, Thursday, June 8, 2006, at the Rayburn House Office Building in Washington. Photo Credit: (NASA/Bill Ingalls)

House Hearing Nunn-McCurdy Review of NPOESS

NASA Image and Video Library

2006-06-07

Chairman of the House Committee on Science and Technology U.S. Rep. Sherwood Boehlert, R-N.Y., questions witnesses during a hearing on the future of the National Polar-orbiting Operational Environmental Satellite System (NPOESS) and results of the Nunn-McCurdy review of NOAA’s weather satellite program, Thursday, June 8, 2006, at the Rayburn House Office Building in Washington. Photo Credit: (NASA/Bill Ingalls)

House Hearing Nunn-McCurdy Review of NPOESS

NASA Image and Video Library

2006-06-07

NASA Administrator Michael Griffin, left, testifies before the House Committee on Science and Technology regarding the future of the National Polar-orbiting Operational Environmental Satellite System (NPOESS) and results of the Nunn-McCurdy review of NOAA’s weather satellite program, as Under Secretary of the Air Force Dr. Ronald Sega looks on, Thursday, June 8, 2006, at the Rayburn House Office Building in Washington. Photo Credit: (NASA/Bill Ingalls)

ff14IDPs Force Field Improving the Conformation Sampling of Intrinsically Disordered Proteins

PubMed Central

Song, Dong; Wang, Wei; Ye, Wei; Ji, Dingjue; Luo, Ray; Chen, Hai-Feng

2017-01-01

Intrinsically disordered proteins (IDPs) are proteins which lack of specific tertiary structure and unable to fold spontaneously without the partner binding. These IDPs are found to associate with various diseases, such as diabetes, cancer, and neurodegenerative diseases. However, current widely used force fields, such as ff99SB, ff14SB, OPLS/AA, and Charmm27 are insufficient in sampling the conformational characters of IDPs. In this study, the CMAP method was used to correct the φ/ψ distributions of disorder-promoting amino acids. The simulation results show that the force filed parameters (ff14IDPs) can improve the φ/ψ distributions of the disorder-promoting amino acids, with RMSD less than 0.10% relative to the benchmark data of IDPs. Further test suggests that the calculated secondary chemical shifts under ff14IDPs force field are in quantitative agreement with the data of NMR experiment for five tested systems. In addition, the simulation results show that ff14IDPs can still be used to model structural proteins, such as tested lysozyme and ubiquitin, with better performance in coil regions than the original general Amber force field ff14SB. These findings confirm that the newly developed Amber ff14IDPs force field is a robust model for improving the conformation sampling of IDPs. PMID:27484738

House Hearing Nunn-McCurdy Review of NPOESS

NASA Image and Video Library

2006-06-08

Under Secretary of the Air Force Dr. Ronald Sega, right, testifies before the House Committee on Science and Technology regarding the future of the National Polar-orbiting Operational Environmental Satellite System (NPOESS) and results of the Nunn-McCurdy review of NOAA’s weather satellite program as NASA Administrator Michael Griffin, left, looks at a television monitor, Thursday, June 8, 2006, at the Rayburn House Office Building in Washington. Photo Credit: (NASA/Bill Ingalls)

House Hearing Nunn-McCurdy Review of NPOESS

NASA Image and Video Library

2006-06-07

Chairman of the House Committee on Science and Technology U.S. Rep. Sherwood Boehlert, R-N.Y., left, shakes hands with NASA Administrator Michael Griffin prior to a hearing on the future of the National Polar-orbiting Operational Environmental Satellite System (NPOESS) and results of the Nunn-McCurdy review of NOAA’s weather satellite program, Thursday, June 8, 2006, at the Rayburn House Office Building in Washington. Photo Credit: (NASA/Bill Ingalls)

House Hearing Nunn-McCurdy Review of NPOESS

NASA Image and Video Library

2006-06-07

NOAA Administrator, Vice Admiral Conrad C. Lautenbacher, Jr., U.S. Navy (Ret.), is seen on a television monitor as he testifies before the House Committee on Science and Technology regarding the future of the National Polar-orbiting Operational Environmental Satellite System (NPOESS) and results of the Nunn-McCurdy review of NOAA’s weather satellite program, Thursday, June 8, 2006, at the Rayburn House Office Building in Washington. Photo Credit: (NASA/Bill Ingalls)

House Hearing Nunn-McCurdy Review of NPOESS

NASA Image and Video Library

2006-06-07

NOAA Administrator, Vice Admiral Conrad C. Lautenbacher, Jr., U.S. Navy (Ret.), left, NASA Administrator Michael Griffin and Under Secretary of the Air Force Dr. Ronald Sega, right, testify before the House Committee on Science and Technology regarding the future of the National Polar-orbiting Operational Environmental Satellite System (NPOESS) and results of the Nunn-McCurdy review of NOAA’s weather satellite program, Thursday, June 8, 2006, at the Rayburn House Office Building in Washington. Photo Credit: (NASA/Bill Ingalls)

House Hearing Nunn-McCurdy Review of NPOESS

NASA Image and Video Library

2006-06-08

NOAA Administrator, Vice Admiral Conrad C. Lautenbacher, Jr., U.S. Navy (Ret.), left, NASA Administrator Michael Griffin and Under Secretary of the Air Force Dr. Ronald Sega, right, testify before the House Committee on Science and Technology regarding the future of the National Polar-orbiting Operational Environmental Satellite System (NPOESS) and results of the Nunn-McCurdy review of NOAA’s weather satellite program, Thursday, June 8, 2006, at the Rayburn House Office Building in Washington. Photo Credit: (NASA/Bill Ingalls)

The NPOESS Preparatory Project (NPP) Science Data Segment (SDS) Data Depository and Distribution Element (SD3E) System Architecture

NASA Technical Reports Server (NTRS)

Ho, Evelyn L.; Schweiss, Robert J.

2008-01-01

supports science mission data assessment by assuring the timely and validated acquisition and subsequent transfer of the NPP Science Mission data to the SDS Elements and NPP Science Team. The six science elements that interface with the SD3E span across the NASA Goddard Space Flight Center (GSFC), the NASA Jet Propulsion Laboratory (JPL), and the University of Wisconsin. As the primary communication vehicle for the science elements and science team, the SD3E has an interface to the operational data providers: National Environment Satellite, Data, and Information Service (NESDIS) Interface Data Processing System (IDPS) and the National Oceanic Atmospheric Administration's (NOAA) Comprehensive Large Array-data Stewardship system (CLASS) Archive Data System (ADS), that are responsible for product generation and archive and distribution respectively. The SD3E is designed to be a semi-customizable and semi-automated system. This system is designed to provide flexibility and ease of use for the science users in accessing the latest data products by creating a rolling data cache that temporarily stores the products locally before transferring the data to the SDS Measurement based elements for the land, ocean, atmosphere, sounder, and ozone. This paper describes the design and architecture of one of the nine SDS elements, the SD3E, and how this system has provided a mechanism for efficient data exchange, how it has helped in alleviating some of the network traffic and usage, and how it has contributed to reducing operational costs.

Modulation of Correlated Segment Fluctuations in IDPs upon Complex Formation as an Allosteric Regulatory Mechanism.

PubMed

Beier, Andreas; Schwarz, Thomas C; Kurzbach, Dennis; Platzer, Gerald; Tribuzio, Francesca; Konrat, Robert

2018-05-05

Molecular recognition of and by intrinsically disordered proteins (IDPs) is an intriguing and still largely elusive phenomenon. Typically, protein recognition involving IDPs requires either folding upon binding or, alternatively, the formation of "fuzzy complexes." Here we show via correlation analyses of paramagnetic relaxation enhancement data unprecedented and striking alterations of the concerted fluctuations within the conformational ensemble of IDPs upon ligand binding. We study the binding of α-synuclein to calmodulin, a ubiquitous calcium-binding protein, and the binding of the extracellular matrix IDP osteopontin to heparin, a mimic of the extracellular matrix ligand hyaluronic acid. In both cases, binding leads to reduction of correlated long-range motions in these two IDPs and thus indicates a loosening of structural compaction upon binding. Most importantly, however, the simultaneous presence of correlated and anti-correlated fluctuations in IDPs suggests the prevalence of "energetic frustration" and provides an explanation for the puzzling observation of disordered allostery in IDPs. Copyright © 2018. Published by Elsevier Ltd.

Transitioning NPOESS Data to Weather Offices: The SPoRT Paradigm with EOS Data

NASA Technical Reports Server (NTRS)

Jedlovec, Gary

2009-01-01

Real-time satellite information provides one of many data sources used by NWS weather forecast offices (WFOs) to diagnose current weather conditions and to assist in short-term forecast preparation. While GOES satellite data provides relatively coarse spatial resolution coverage of the continental U.S. on a 10-15 minute repeat cycle, polar orbiting imagery has the potential to provide snapshots of weather conditions at high-resolution in many spectral channels. Additionally, polar orbiting sounding data can provide additional information on the thermodynamic structure of the atmosphere in data sparse regions of at asynoptic observation times. The NASA Short-term Prediction Research and Transition (SPoRT) project has demonstrated the utility of polar orbiting MODIS and AIRS data on the Terra and Aqua satellites to improve weather diagnostics and short-term forecasting on the regional and local scales. SPoRT scientists work directly forecasters at selected WFOS in the Southern Region (SR) to help them ingest these unique data streams into their AWIPS system, understand how to use the data (through on-site and distance learn techniques), and demonstrate the utility of these products to address significant forecast problems. This process also prepares forecasters for the use of similar observational capabilities from NPOESS operational sensors. NPOESS environmental data records (EDRs) from the Visible 1 Infrared Imager I Radiometer Suite (VIIRS), the Cross-track Infrared Sounder (CrlS) and Advanced Technology Microwave Sounder (ATMS) instruments and additional value-added products produced by NESDIS will be available in near real-time and made available to WFOs to extend their use of NASA EOS data into the NPOESS era. These new data streams will be integrated into the NWs's new AWIPS II decision support tools. The AWIPS I1 system to be unveiled in WFOs in 2009 will be a JAVA-based decision support system which preserves the functionality of the existing systems and

AURORA: The Next Generation Space Weather Sensor for NPOESS

NASA Astrophysics Data System (ADS)

Paxton, L.; Morrison, D.; Santo, A.; Ogorzalek, B.; Goldsten, J.; Boldt, J.; Kil, H.; Zhang, Y.; Demajistre, R.; Wolven, B.; Meng, C.

2005-12-01

The AURORA sensor slated for flight on the NPOESS satellites represents the culmination of over 20 years of experience at JHU/APL in the design, manufacture, flight, operation and analysis of compact, cost-effective far ultraviolet sensors for space weather data collection. The far ultraviolet covers the spectral range from about 115 to 185 nm. This region is ideal for observations of the upper atmosphere because, at these wavelengths, the lower atmosphere and Earth's surface are black. AURORA will observe the mid- and low-latitude F-region ionosphere, the auroral E-region ionosphere, the day thermosphere composition, auroral energy deposition and map ionospheric irregularities. AURORA implements the flight-proven design derived from SSUSI on the DMSP Block 5D spacecraft and GUVI on the NASA TIMED spacecraft. These instruments have provided the instrument and algorithm heritage for NPOESS/AURORA. In this talk the performance capabilities of the AURORA instrument will be summarized along with the design of the instrument and algorithms. Example products will be shown for each of the measurement regimes. We acknowldge support from DMSP and NASA and the collaboration with our science colleagues at the Aerospace Corporation (Paul Straus, Jim Hecht, Dave McKenzie, and Andy Christensen) and Computational Physics (Doug Strickland, Hal Knight, and Scott Evans) and Naval Research Laboratory (Robert Meier, Mike Picone, Stefan Thonnard, Pat Dandenault, and Andy Stefan) and our colleagues at APL (Michele Weiss, Doug Holland, Bill Wood, and Jim Eichert) among others.

Carbon analyses of IDP's sectioned in sulfur and supported on beryllium films

NASA Technical Reports Server (NTRS)

Bradley, J. P.; Keller, L.; Thomas, K. L.; Vanderwood, T. B.; Brownlee, D. E.

1993-01-01

Carbon is the only major element in interplanetary dust whose abundance, distribution and chemical state are not well understood. Information about carbon could clarify the relationship between the various classes of IDP's, conventional meteorites, and sources (e.g., comets vs. asteroids). To date, the most reliable estimates of C abundance in Interplanetary Dust Particles (IDP's) have been obtained by analyzing particles on thick-flat Be substrates using thin-window energy-dispersive spectroscopy in the SEM. These estimates of C abundance are valid only if C is homogeneously distributed, because detected C x-rays originate from the outer 0.1 micrometers of the particle. An alternative and potentially more accurate method of measuring C abundances is to analyze multiple thin sections (each less than 0.1 less than 0.1 micrometers thick) of IDP's. These efforts however, have been stymied because of a lack of a suitable non-carbonaceous embedding medium and the availability of C-free conductive substrates. We have embedded and thin-sectioned IDP's in glassy sulfur, and transferred the thin sections to Be support films approximately 25 nm thick. The sections were then analyzed in a 200 KeV analytical TEM. S sublimes rapidly under vacuum in the TEM, leaving non-embedded sections supported on Be. Apart from quantitative C (and O) analyses, S sectioning dramatically expands the range of analytical measurements that can be performed on a single IDP.

The Expected Impacts of NPOESS Microwave and Infrared Sounder Radiances on Operational Numerical Weather Prediction and Data Assimilation Systems

NASA Astrophysics Data System (ADS)

Swadley, S. D.; Baker, N.; Derber, J.; Collard, A.; Hilton, F.; Ruston, B.; Bell, W.; Candy, B.; Kleespies, T. J.

2009-12-01

The NPOESS atmospheric sounding functionality will be accomplished using two separate sensor suites, the combined infrared (IR) and microwave (MW) sensor suite (CrIMSS), and the Microwave Imager/Sounder (MIS) instrument. CrIMSS consists of the Cross Track Infrared Sounder (CrIS) and the cross track Advanced Technology Microwave Sounder (ATMS), and is scheduled to fly on the NPOESS Preparatory Project (NPP), and NPOESS operational flight units C1 and C3. The MIS is a conical scanning polarimetric imager and sounder patterned after the heritage WindSat, and DMSP Special Sensor Microwave Imagers and Sounders (SSMI and SSMIS), and is scheduled for flight units C2, C3 and C4. ATMS combines the current operational Advanced Microwave Sounding Unit (AMSU) and the Microwave Humidity Sounder (MHS), but with an additional channel in the 51.76 GHz oxygen absorption region and 3 additional channels in the 165.5 and 183 GHz water vapor absorption band. CrIS is a Fourier Transform Spectrometer and will provide 159 shortwave IR channels, 433 mid-range IR channels, and 713 longwave IR channels. The heritage sensors for CrIS are the NASA Advanced Infrared Sounder (AIRS) and the MetOp-A Infrared Atmospheric Sounding Interferometer (IASI). Both AIRS and IASI are high quality, high spectral resolution sounders which represent a significant improvement in the effective vertical resolution over previous IR sounders. This presentation will give an overview of preparations underway for day-1 monitoring of NPP/NPOESS radiances, and subsequent operational radiance assimilation. These preparations capitalize on experience gained during the pre-launch preparations, sensor calibration/validation and operational assimilation for the heritage sensors. One important step is to use pre-flight sensor channel specifications, noise estimates and knowledge of the antenna patterns, to generate and test proxy NPP/NPOESS sensor observations in existing assimilation systems. Other critical factors for

Diversity and adaptation of shelters in transitional settlements for IDPs in Afghanistan.

PubMed

Ashmore, Joseph; Babister, Elizabeth; Corsellis, Tom; Fowler, Jon; Kelman, Ilan; McRobie, Allan; Manfield, Peter; Spence, Robin; Vitale, Antonella; Battilana, Rachel; Crawford, Kate

2003-12-01

The diversity of shelters used in transitional settlements for internally displaced persons (IDPs) in Herat, Afghanistan is described. The information is based on a field survey undertaken in March 2002 and highlights the adaptation techniques, which IDPs undertake to improve any provided shelter. Potential areas for improvement are indicated; for example, the possibility for using insulated, demountable liners to prevent cold-related deaths without sacrificing shelter flexibility along with the likely need for better agency coordination of the shelter responses they provide. The wider context in which the technical recommendations would be implemented must also be considered. Such issues include agency resources, political impediments to providing the desired option, and the preference of many IDPs that the best shelter would be their home.

N-15-Rich Organic Globules in a Cluster IDP and the Bells CM2 Chondrite

NASA Technical Reports Server (NTRS)

Messenger, S.; Nakamura-Messenger, K.; Keller, Lindsay P.

2008-01-01

Organic matter in primitive meteorites and chondritic porous interplanetary dust particles (CP IDPs) is commonly enriched in D/H and 15N/14N relative to terrestrial values [1-3]. These anomalies are ascribed to the partial preservation of presolar cold molecular cloud material [1]. Some meteorites and IDPs contain m-size inclusions with extreme H and N isotopic anomalies [2-4], possibly due to preserved pristine primordial organic grains. We recently showed that the in the Tagish Lake meteorite, the principle carriers of these anomalies are sub- m, hollow organic globules [5]. The globules likely formed by photochemical processing of organic ices in a cold molecular cloud or the outermost regions of the protosolar disk [5]. We proposed that similar materials should be common among primitive meteorites, IDPs, and comets. Similar objects have been observed in organic extracts of carbonaceous chondrites [6-8], however their N and H isotopic compositions are generally unknown. Bulk H and N isotopic compositions may indicate which meteorites best preserve interstellar organic compounds. Thus, we selected the Bells CM2 carbonaceous chondrites for study based on its large bulk 15N (+335 %) and D (+990 %) [9].

Nebular and Interstellar Materials in a Giant Cluster IDP of Probable Cometary Origin

NASA Technical Reports Server (NTRS)

Messenger, S.; Brownlee, D. E.; Joswiak, D. J.; Nguyen, A. N.

2015-01-01

Comets contain a complex mixture of materials with presolar and Solar System origins. Chondritic porous interplanetary dust particles (CP-IDPs) are associated with comets by their fragile nature, unequilibrated anhydrous mineralogy and high abundances of circumstellar grains and isotopically anomalous organic materials. Comet 81P/Wild 2 samples returned by the Stardust spacecraft contain presolar materials as well as refractory 16O-rich Ca-Al-rich inclusion- (CAI), chondrule-, and AOA-like materials. We are conducting coordinated chemical, mineralogical, and isotopic studies of a giant cluster CP-IDP (U2-20-GCA) to determine the proportions of inner Solar System and interstellar materials. We previously found that this IDP contains abundant presolar silicates (approx. 1,800 ppm) and 15N-rich hotspots [6].

Spatially Resolved Analysis of Amines Using a Fluorescence Molecular Probe: Molecular Analysis of IDPs

NASA Technical Reports Server (NTRS)

Clemett, S. J.; Messenger, S.; Thomas-Keprta, K. L.; Wentworth, S. J.; Robinson, G. A.; McKay, D. S.

2002-01-01

Some Interplanetary Dust Particles (IDPs) have large isotope anomalies in H and N. To address the nature of the carrier phase, we are developing a procedure to spatially resolve the distribution of organic species on IDP thin sections utilizing fluorescent molecular probes. Additional information is contained in the original extended abstract.

UV production of methane from surface and sedimenting IDPs on Mars in light of REMS data and with insights for TGO

NASA Astrophysics Data System (ADS)

Moores, John E.; Smith, Christina L.; Schuerger, Andrew C.

2017-11-01

This paper refines model predictions for the production of methane from UV-irradiated interplanetary dust particles (IDPs) now that the Rover Environmental Monitoring Station (REMS) instrument onboard the Mars Science Laboratory (MSL) Rover has made the first measurements of the UV environment on the surface of Mars, at Gale Crater. Once these measurements are included in a UV radiative transfer model, we find that modelled UV sol-integrated energies across the planet are lower than pre-measurement estimates by 35% on average, considering all latitudes and seasons. This reduction, in turn, reduces the predicted production of methane from individual accreting IDPs, extending their lifetimes and increasing the surface concentration of organics that must accumulate in order to emit sufficient methane to balance the accretion of organic compounds to Mars. Emission from reasonable accumulations of IDPs could range up to ∼7.9 × 10-4 ppbv sol-1. Richer deposits of organic carbon at the surface may emit methane at no more than 3.9 ppbv sol-1. An examination of IDP-derived methane production during atmospheric settling indicates that no more than 0.32% of organic carbon from meteor streams may be deposited in the atmosphere. Thus, such a process cannot explain either the spikes observed in methane nor the low equilibrium values observed by MSL. Instead, this discrepancy may be explained if < 80 tons per year of organic carbon survives to the surface, the atmospheric lifetime of methane is < 110 years or the efficiency of the UV-CH4 process is <7%. Under the assumption of reduced carbon input cycling in the Martian system from these processes, both soil concentrations of organic carbon and atmospheric measurements of methane observed by MSL are consistent with the UV-CH4 process. This refinement of methane production from IDPs and its geographical and vertical distribution will be an important input for models attempting to understand the results to be derived from the

The Abundance and Distribution of Presolar Materials in Cluster IDPS

NASA Technical Reports Server (NTRS)

Messenger, Scott; Keller, Lindsay; Nakamura-Messenger, Keiko; Ito, Motoo

2007-01-01

Presolar grains and remnants of interstellar organic compounds occur in a wide range of primitive solar system materials, including meteorites, interplanetary dust particles (IDPs), and comet Wild-2 samples. Among the most abundant presolar phases are silicate stardust grains and molecular cloud material. However, these materials have also been susceptible to destruction and alteration during parent body and nebular processing. In addition to their importance as direct samples of remote and ancient astrophysical environments, presolar materials thus provide a measure of how well different primitive bodies have preserved the original solar system starting materials.

An automated procedure for detection of IDP's dwellings using VHR satellite imagery

NASA Astrophysics Data System (ADS)

Jenerowicz, Malgorzata; Kemper, Thomas; Soille, Pierre

2011-11-01

This paper presents the results for the estimation of dwellings structures in Al Salam IDP Camp, Southern Darfur, based on Very High Resolution multispectral satellite images obtained by implementation of Mathematical Morphology analysis. A series of image processing procedures, feature extraction methods and textural analysis have been applied in order to provide reliable information about dwellings structures. One of the issues in this context is related to similarity of the spectral response of thatched dwellings' roofs and the surroundings in the IDP camps, where the exploitation of multispectral information is crucial. This study shows the advantage of automatic extraction approach and highlights the importance of detailed spatial and spectral information analysis based on multi-temporal dataset. The additional data fusion of high-resolution panchromatic band with lower resolution multispectral bands of WorldView-2 satellite has positive influence on results and thereby can be useful for humanitarian aid agency, providing support of decisions and estimations of population especially in situations when frequent revisits by space imaging system are the only possibility of continued monitoring.

Element Mapping in Anhydrous IDPs: Identification of the Host Phases of Major/Minor Elements as a Test of Nebula Condensation Models

NASA Technical Reports Server (NTRS)

Flynn, G. J.; Keller, L. P.; Wirick, S.; Jacobsen, C.

2004-01-01

Many anhydrous interplanetary dust particles (IDPs) are the most pristine samples of primitive solar system dust currently available for laboratory analysis. Their primitive nature is demonstrated by: 1) the high content of moderately volatile elements, indicating they have not been heated significantly since formation, 2) the absence of hydrated material, indicating they never experienced aqueous processing, 3) the presence of unequilibrated mineral assemblages, 4) the presence of large isotopic anomalies (e.g., D and 15N enrichment), in these IDPs.

Isotopic and Trace Element Compositions of Antarctic Micrometeorites and Comparison with IDPs

NASA Astrophysics Data System (ADS)

Stadermann, F. J.; Olinger, C. T.

1992-07-01

Antarctic micrometeorites (AMMs) show resemblances and differences to both stratospheric interplanetary dust particles (IDPs) and chondritic meteorites, but the exact nature of this relationship has yet to be established. We measured Ne, H, C, and N isotopic compositions, as well as trace element abundances in several AMMs in order to compare the results to similar measurements of IDPs (Stadermann, 1991). AMMs for this study were collected near Cap-Prudhomme (Maurette et al., 1989), and optically selected (Olinger et al., 1990). Noble gases of 23 selected AMMs were extracted through laser vaporization. Nine of these particles contained implanted solar Ne and one showed a clear signature from spallogenic Ne, confirming their extraterrestrial origin. We selected fragments from 6 of these particles, plus 2 containing apparent Ne excess and one with a roughly chondritic bulk chemistry but immeasurably low Ne, for further analyses. Secondary ion mass spectrometry (SIMS) was used to measure the H, C, and N isotopic compositions. These measurements turned out to be difficult, since the concentrations of H and C in the analyzed samples were significantly lower than in IDPs. The low concentration of C also affected the N isotopic measurements because N could only be measured as CN-. We were able to measure H in 9, as well as C and N in 3 AMMs. All measurements yielded isotopically normal results. Previous determinations of the O isotopic compositions of the same samples (Virag, pers. comm.) also gave no indication of isotopic anomalies. These results are significantly different from measurements of IDPs, where isotopic anomalies in H and N were found in roughly 1/2 and 1/3 of the particles, respectively. SIMS was also used to measure the rare earth and trace element abundances in up to 4 different fragments of 6 AMMs. Although most particles had roughly chondritic abundances, anomalous concentrations were found for Ca, Li, Co, Ni, and Ba. Significant Ca depletions up to 0

Chemical Heterogeneity of a Large Cluster IDP: Clues to its Formation History Using X-ray Fluorescence Mapping and XANES Spectroscopy

NASA Technical Reports Server (NTRS)

Wirick, S.; Flynn, G. J.; Sutton, S.; Zolensky, M. E.

2013-01-01

Chondritic porous IDPs may be among the most primitive objects found in our solar system [1]. They consist of many micron to submicron minerals, glasses and carbonaceous matter [2,3,4,5,6,7] with > 10(exp 4) grains in a 10 micron cluster [8]. Speculation on the environment where these fine grained, porous IDPs formed varies with possible sources being presolar dusty plasma clouds, protostellar condensation, solar asteroids or comets [4,6,9]. Also, fine grained dust forms in our solar system today [10,11]. Isotopic anomalies in some particles in IDPs suggest an interstellar source[4,7,12]. IDPs contain relic particles left from the dusty plasma that existed before the protostellar disk formed and other grains in the IDPs formed later after the cold dense nebula cloud collapsed to form our protostar and other grains formed more recently. Fe and CR XANES spectroscopy is used here to investigate the oxygen environment in a large (>50 10 micron or larger sub-units) IDP. Conclusions: Analyzing large (>50 10 micron or larger sub-units) CP IDPs gives one a view on the environments where these fine dust grains formed which is different from that found by only analyzing the small, 10 micron IDPs. As with cluster IDP L2008#5 [3], L2009R2 cluster #13 appears to be an aggregate of grains that sample a diversity of solar and perhaps presolar environments. Sub-micron, grain by grain measurement of trace element contents and elemental oxidation states determined by XANES spectroscopy offers the possibility of understanding the environments in which these grains formed when compared to standard spectra. By comparing thermodynamic modeling of condensates with analytical data an understanding of transport mechanisms operating in the early solar system may be attained.

What does the fine-scale petrography of IDPs reveal about grain formation and evolution in the early solar system?

NASA Technical Reports Server (NTRS)

Bradley, John

1994-01-01

The 'pyroxene' interplanetary dust particles (IDP's) may be the best samples for investigation of primordial grain-forming reactions because they appear to have experienced negligible post-accretional alteration. They are likely to continue to yield information about gas-to-solid condensation and other grain-forming reactions that may have occurred either in the solar nebular or presolar interstellar environments. An immediate challenge lies in understanding the nanometer-scale petrography of the ultrafine-grained aggregates in 'pyroxene' IDP's. Whether these aggregates contain components from diverse grain-forming environments may ultimately be answered by systematic petrographic studies using electron microscopes capable of high spatial resolution microanalysis. It may be more difficult to decipher evidence of grain formation and evolution in 'olivine' and 'layer silicate' IDP's because they appear to have experienced post-accretional alteration. Most of the studied 'olivine' IDPs have been subjected to heating and equilibration, perhaps during atmospheric entry, while the 'layer silicate' IDP's have experienced aqueous alteration.

Phosphorylation of the IDP KID Modulates Affinity for KIX by Increasing the Lifetime of the Complex.

PubMed

Dahal, Liza; Shammas, Sarah L; Clarke, Jane

2017-12-19

Intrinsically disordered proteins (IDPs) are known to undergo a range of posttranslational modifications, but by what mechanism do such modifications affect the binding of an IDP to its partner protein? We investigate this question using one such IDP, the kinase inducible domain (KID) of the transcription factor CREB, which interacts with the KIX domain of CREB-binding protein upon phosphorylation. As with many other IDPs, KID undergoes coupled folding and binding to form α-helical structure upon interacting with KIX. This single site phosphorylation plays an important role in the control of transcriptional activation in vivo. Here we show that, contrary to expectation, phosphorylation has no effect on association rates-unphosphorylated KID binds just as rapidly as pKID, the phosphorylated form-but rather, acts by increasing the lifetime of the complex. We propose that by controlling the lifetime of the bound complex of pKID:KIX via altering the dissociation rate, phosphorylation can facilitate effective control of transcription regulation. Copyright © 2017 Biophysical Society. Published by Elsevier Inc. All rights reserved.

Collection and curation of IDPs in the stratosphere and below. Part 2: The Greenland and Antarctic ice sheets

NASA Technical Reports Server (NTRS)

Maurette, Michel; Hammer, C.; Harvey, R.; Immel, G.; Kurat, G.; Taylor, S.

1994-01-01

In a companion paper, Zolensky discusses interplanetary dust particles (IDP's) collected in the stratosphere. Here, we describe the recovery of much larger unmelted to partially melted IDP's from the Greenland and Antarctica ice sheet, and discuss problems arising in their collection and curation, as well as future prospects for tackling these problems.

Focused Ion Beam Recovery and Analysis of Interplanetary Dust Particles (IDPs) and Stardust Analogues

NASA Technical Reports Server (NTRS)

Graham, G. A.; Bradley, J. P.; Bernas, M.; Stroud, R. M.; Dai, Z. R.; Floss, C.; Stadermann, F. J.; Snead, C. J.; Westphal, A. J.

2004-01-01

Meteoritics research is a major beneficiary of recent developments in analytical instrumentation [1,2]. Integrated studies in which multiple analytical techniques are applied to the same specimen are providing new insight about the nature of IDPs [1]. Such studies are dependent on the ability to prepare specimens that can be analyzed in multiple instruments. Focused ion beam (FIB) microscopy has revolutionized specimen preparation in materials science [3]. Although FIB has successfully been used for a few IDP and meteorite studies [1,4-6], it has yet to be widely utilized in meteoritics. We are using FIB for integrated TEM/NanoSIMS/synchrotron infrared (IR) studies [1].

Comparing Wild 2 Particles to Chondrites and IDPS

NASA Technical Reports Server (NTRS)

Zolensky, Michael; Nakamura-Messenger, Keiko; Rietmeijer, Frans; Leroux, Hugues; Mikouchi, Takashi; Ohsumi, Kazumasa; Simon, Steven; Grossman, Lawrence; Stephan, Thomas; Weisberg, Michael;

Long-term effects of traumatic experience: Comparison study in the adolescent IDPs in Serbia.

PubMed

Matsunaga, Chieko; Ristic, Dragana; Niregi, Mitsuki

2006-12-01

The purpose of this study is to examine the long term psychological effects of war stress regarded as traumatic experience. The subjects are Serbian internally displaced people (IDP) of adolescent population from Kosovo. It is a very big concern whether the adolescents would overcome the social and psychological difficulties caused by the war stress in order to reconstruct the better society. The result came out that the long-term effects still exist in PTSD, depression and hopelessness, which affects self-esteem and the attitude in purpose in life that are important factors for personality development. This paper also examines the difference between IDPs with war stress and the adolescent sufferers of the big earthquake in Japan.

Depletions of sulfur and/or zinc in IDPs: Are they reliable indicators of atmospheric entry heating?

NASA Technical Reports Server (NTRS)

Flynn, G. J.; Sutton, S. R.; Bajt, S.; Kloeck, W.; Thomas, K. L.; Keller, L. P.

1993-01-01

The degree of heating of interplanetary dust particles (IDP's) on Earth atmospheric entry is important in distinguishing cometary particles from main-belt asteroidal particles. Depletions in the volatile elements S and Zn were proposed as chemical indicators of significant entry heating. The S and Zn contents of cosmic dust particles were correlated with physical indicators of atmospheric entry heating, such as the production of magnetite and the loss of solar wind implanted He. The results indicate that the Zn content of IDP's is a useful indicator of entry heating, but the S content seems to be less useful.

NASA's NPOESS Preparatory Project Science Data Segment: A Framework for Measurement-based Earth Science Data Systems

NASA Technical Reports Server (NTRS)

Schwaller, Mathew R.; Schweiss, Robert J.

2007-01-01

The NPOESS Preparatory Project (NPP) Science Data Segment (SDS) provides a framework for the future of NASA s distributed Earth science data systems. The NPP SDS performs research and data product assessment while using a fully distributed architecture. The components of this architecture are organized around key environmental data disciplines: land, ocean, ozone, atmospheric sounding, and atmospheric composition. The SDS thus establishes a set of concepts and a working prototypes. This paper describes the framework used by the NPP Project as it enabled Measurement-Based Earth Science Data Systems for the assessment of NPP products.

Dissemination and Use of NPOESS Data in AWIPS II

NASA Technical Reports Server (NTRS)

Jedlovec, Gary; Burks, Jason

2008-01-01

Real-time satellite information provides one of many data sources used by NWS forecast offices to diagnose current weather conditions and to assist in short-term forecast preparation. While GOES satellite data provides relatively coarse spatial resolution coverage of the continental U.S. on a 10-15 minute repeat cycle, polar orbiting data has the potential to provide snapshots of weather conditions at high-resolution in many spectral channels. The multispectral polar orbiting satellite capabilities allow for the derivation of image and sounding products not available from geostationary orbit. The utility of these polar orbiting measurements to forecasters has been demonstrated with NASA EOS observations as part of the Short-term Prediction and Research Transition (SPORT) program at Marshall Space Flight Center. SPORT scientists have been providing real-time MODIS data to NWS forecasters on an experimental basis to address a variety of short-term weather forecasting problems since 2003. The launch of the NPOESS Preparatory Project (NPP) satellite in 2009 will extend the continuity of high-resolution data provided by the NASA EOS satellites into future operational weather systems. The NPP data will be available in a timeframe consistent with the early installation of the next generation Advanced Weather Information Processing System (AWIPS) under development by Raytheon for the NWS. The AWIPS II system will be a JAVA-based decision support system which preserves the functionality of the existing systems and offers unique development opportunities for new data sources and applications in the Service Orientated Architecture (SOA) environment. The poster will highlight some of the advanced observing and display- capabilities of these new systems such as plug-ins for NASA and NPP datasets, and the development of local applications which are not well handled in the current AWIPS (e.g., 3D displays of LMA data, generation and display of 3-channel color composites, etc.).

The NPOESS Preparatory Project Science Data Segment (SDS) Data Depository and Distribution Element (SD3E) System Architecture

NASA Technical Reports Server (NTRS)

Ho, Evelyn L.; Schweiss, Robert J.

2008-01-01

The National Polar-orbiting Operational Environmental Satellite System (NPOESS) Preparatory Project (NPP) Science Data Segment (SDS) will make daily data requests for approximately six terabytes of NPP science products for each of its six environmental assessment elements from the operational data providers. As a result, issues associated with duplicate data requests, data transfers of large volumes of diverse products, and data transfer failures raised concerns with respect to the network traffic and bandwidth consumption. The NPP SDS Data Depository and Distribution Element (SD3E) was developed to provide a mechanism for efficient data exchange, alleviate duplicate network traffic, and reduce operational costs.

Exploiting Satellite Remote-Sensing Data in Fine Particulate Matter Characterization for Serving the Environmental Public Health Tracking Network (EPHTN): The HELIX-Atlanta Experience and NPOESS Implications

NASA Technical Reports Server (NTRS)

Al-Hamdan, Mohammad Z.; Crosson, William L.; Limaye, Ashutosh S.; Rickman, Douglas L.; Quattrochi, Dale A.; Estes, Maurice G.; Qualters, Judith R.; Sinclair, Amber H.; Tolsma, Dennis D.; Adeniyi, Kafayat A.

2008-01-01

As part of the U.S. National Environmental Public Health Tracking Network (EPHTN), the National Center for Environmental Health (NCEH) at the U.S. Centers for Disease Control and Prevention (CDC) led a project in collaboration with the National Aeronautics and Space Administration (NASA) Marshall Space Flight Center (MSFC) called Health and Environment Linked for Information Exchange (HELIX-Atlanta). Under HELIX-Atlanta, pilot projects were conducted to develop methods to better characterize exposure; link health and environmental datasets; and analyze spatial/temporal relationships. This paper describes and demonstrates different techniques for surfacing daily environmental hazards data of particulate matter with aerodynamic diameter less than or equal to 2.5 micrometers (PM(sub 2.5) for the purpose of integrating respiratory health and environmental data for the CDC's pilot study of HELIX-Atlanta. It describes a methodology for estimating ground-level continuous PM(sub 2.5) concentrations using spatial surfacing techniques and leveraging NASA Moderate Resolution Imaging Spectrometer (MODIS) data to complement the U.S. Environmental Protection Agency (EPA) ground observation data. The study used measurements of ambient PM(sub 2.5) from the EPA database for the year 2003 as well as PM(sub 2.5) estimates derived from NASA's MODIS data. Hazard data have been processed to derive the surrogate exposure PM(sub 2.5) estimates. The paper has shown that merging MODIS remote sensing data with surface observations of PM(sub 2.5), may provide a more complete daily representation of PM(sub 2.5), than either data set alone would allow, and can reduce the errors in the PM(sub 2.5) estimated surfaces. Future work in this area should focus on combining MODIS column measurements with profile information provided by satellites like the National Polar-orbiting Operational Environmental Satellite System (NPOESS). The Visible Infrared Imager/Radiometer Suite (VIIRS) and the Aerosol

Spatially Resolved Acid Dissolution of IDPs: The State of Carbon and the Abundance of Diamonds in the Dust

NASA Technical Reports Server (NTRS)

Brownlee, D. E.; Joswiak, D. J.; Bradley, J. P.; Gezo, J. C.; Hill, H. G. M.

2000-01-01

Ultramicrotome sections of IDPs have been successfully etched with HF to isolate and reveal the microdistribution of carbonaceous material. The sections are evaluated for nanodiamonds, 3.4 micron feature, GEMS and the origin of low albedo in small interplanetary particles.

Assisting Groundwater Exploration for Refugee/IDP Camps by Remote Sensing and GIS

NASA Astrophysics Data System (ADS)

Wendt, Lorenz; Robl, Jörg; Hilberg, Sylke; Braun, Andreas; Rogenhofer, Edith; Dirnberger, Daniel; Strasser, Thomas; Füreder, Petra; Lang, Stefan

2015-04-01

Refugee camps and camps of internally displaced people (IDP) often form spontaneously or have to be established rapidly in remote, rural areas, where little is known about the hydrogeological situation. This requires a rapid assessment of the availability of groundwater to enable humanitarian organisations like Médecins Sans Frontières (MSF) to supply the camp population with sufficient potable water. Within the project EO4HumEn, hydrogeological reconnaissance maps are produced for MSF by integrating remote sensing data like SRTM, Landsat, ASTER, optical very-high resolution (VHR) imagery, and SAR data. Depending on the specific situation of the camps, these maps contain topography, permanent and temporary water bodies, hard rock outcrops and their geological variability, locations of existing boreholes and wells (if available), potential contamination sources, roads and obstacles (e.g. swampland). In areas characterized by unconsolidated sediments, specific landforms like alluvial fans, meanders, levees, deltas or beach ridges are identified. Here, the reconnaissance map can be sufficient to plan drill sites for groundwater abstraction. In hard rock areas, the lithology is determined, if the vegetation cover allows it. Fractures, faults and karst features are mapped to resolve the structural setting. Anomalous vegetation patterns are interpreted in terms of near-surface groundwater. The maps provide an overview of the camp surroundings, and allow the field hydrogeologists to focus their investigations on the most promising locations. The maps are complemented by a literature review on geological maps, articles and reports available for the area of interest. Assisting groundwater exploration by remote sensing data analysis is not a new development, but it has not been widely adopted by the humanitarian community as interfaces between humanitarian organisations and GI-scientists were missing. EO4HumEn fills this gap by a strong interdisciplinary cooperation

Sensitivity of Short-Term Weather Forecasts to Assimilated AIRS Data: Implications for NPOESS Applications

NASA Technical Reports Server (NTRS)

Zavodsky, Bradley; McCarty, Will; Chou, Shih-Hung; Jedlovec, Gary

2009-01-01

The Atmospheric Infrared Sounder (AIRS) is acting as a heritage and risk reduction instrument for the Cross-track lnfrared Sounder (CrIS) to be flown aboard the NPP and NPOESS satellites. The hyperspectral nature of AIRS and CrIS provides high-quality soundings that, along with their asynoptic observation time over North America, make them attractive sources to fill the spatial and temporal data voids in upper air temperature and moisture measurements for use in data assimilation and numerical weather prediction. Observations from AlRS can be assimilated either as direct radiances or retrieved thermodynamic profiles, and the Short-Term Prediction Research and Transition (SPORT) Center at NASA's Marshall Space Flight Center has used both data types to improve short-term (0-48h), regional forecasts. The purpose of this paper is to share SPORT'S experiences using AlRS radiances and retrieved profiles in regional data assimilation activities by showing that proper handling of issues-including cloud contamination and land emissivity characterization-are necessary to produce optimal analyses and forecasts.

McIDAS-V: Data Analysis and Visualization for NPOESS and GOES-R

NASA Astrophysics Data System (ADS)

Rink, T.; Achtor, T. H.

2009-12-01

McIDAS-V, the next-generation McIDAS, is being built on top a modern, cross-platform software framework which supports development of 4-D, interactive displays and integration of wide-array of geophysical data. As the replacement of McIDAS, the development emphasis is on future satellite observation platforms such as NPOESS and GOES-R. Data interrogation, analysis and visualization capabilities have been developed for multi- and hyper-spectral instruments like MODIS, AIRS and IASI, and are being extended for application to VIIRS and CrIS. Compatibility with GOES-R ABI level1 and level2 product storage formats has been demonstrated. The abstract data model, which can internalize most any geophysical data, opens up new possibilities for data fusion techniques, for example, polar and geostationary, (LEO/GEO), synergy for research and validation. McIDAS-V follows an object-oriented design model, using the Java programming language, allowing specialized extensions for for new sources of data, and novel displays and interactive behavior. The reference application, what the user sees on startup, can be customized, and the system has a persistence mechanism allowing sharing of the application state across the internet. McIDAS-V is open-source, and free to the public.

Contributions of the SDR Task Network tool to Calibration and Validation of the NPOESS Preparatory Project instruments

NASA Astrophysics Data System (ADS)

Feeley, J.; Zajic, J.; Metcalf, A.; Baucom, T.

2009-12-01

The National Polar-orbiting Operational Environmental Satellite System (NPOESS) Preparatory Project (NPP) Calibration and Validation (Cal/Val) team is planning post-launch activities to calibrate the NPP sensors and validate Sensor Data Records (SDRs). The IPO has developed a web-based data collection and visualization tool in order to effectively collect, coordinate, and manage the calibration and validation tasks for the OMPS, ATMS, CrIS, and VIIRS instruments. This tool is accessible to the multi-institutional Cal/Val teams consisting of the Prime Contractor and Government Cal/Val leads along with the NASA NPP Mission team, and is used for mission planning and identification/resolution of conflicts between sensor activities. Visualization techniques aid in displaying task dependencies, including prerequisites and exit criteria, allowing for the identification of a critical path. This presentation will highlight how the information is collected, displayed, and used to coordinate the diverse instrument calibration/validation teams.

Sensor System Performance Evaluation and Benefits from the NPOESS Airborne Sounder Testbed-Interferometer (NAST-I)

NASA Technical Reports Server (NTRS)

Larar, A.; Zhou, D.; Smith, W.

2009-01-01

Advanced satellite sensors are tasked with improving global-scale measurements of the Earth's atmosphere, clouds, and surface to enable enhancements in weather prediction, climate monitoring, and environmental change detection. Validation of the entire measurement system is crucial to achieving this goal and thus maximizing research and operational utility of resultant data. Field campaigns employing satellite under-flights with well-calibrated FTS sensors aboard high-altitude aircraft are an essential part of this validation task. The National Polar-orbiting Operational Environmental Satellite System (NPOESS) Airborne Sounder Testbed-Interferometer (NAST-I) has been a fundamental contributor in this area by providing coincident high spectral/spatial resolution observations of infrared spectral radiances along with independently-retrieved geophysical products for comparison with like products from satellite sensors being validated. This paper focuses on some of the challenges associated with validating advanced atmospheric sounders and the benefits obtained from employing airborne interferometers such as the NAST-I. Select results from underflights of the Aqua Atmospheric InfraRed Sounder (AIRS) and the Infrared Atmospheric Sounding Interferometer (IASI) obtained during recent field campaigns will be presented.

The NPOESS Crosstrack Infrared Sounder (CrIS) and Advanced Technology Microwave Sounder (ATMS) as a Companion to the New Generation AIRS/AMSU and IASI/AMSU Sounder Suites

NASA Astrophysics Data System (ADS)

Bingham, G. E.; Pougatchev, N. S.; Zavyalov, V.; Esplin, M.; Blackwell, W. J.; Barnet, C.

2009-12-01

The NPOESS Preparatory Project is serving the operations and research community as the bridge mission between the Earth Observing System and the National Polar-orbiting Operational Environmental Satellite System. The Cross-track Infrared Sounder (CrIS), combined with the Advanced Technology Microwave Sounder (ATMS) are the core instruments to provide the key performance temperature and humidity profiles (along with some other atmospheric constituent information). Both the high spectral resolution CrIS and the upgraded microwave sounder (ATMS) will be working in parallel with already orbiting Advanced Atmospheric Infrared Sounder (AIRS/AMSU) on EOS AQUA platform and Infrared Atmospheric Sounding Interferometer (IASI/AMSU) on METOP-A satellite. This presentation will review the CrIS/ATMS capabilities in the context of continuity with the excellent performance records established by AIRS and IASI. The CrIS sensor is in the process of its final calibration and characterization testing and the results and Sensor Data Record process are being validated against this excellent dataset. The comparison between CrIS, AIRS, and IASI will include spectral, spatial, radiometric performance and sounding capability comparisons.

Improving the automated detection of refugee/IDP dwellings using the multispectral bands of the WorldView-2 satellite

NASA Astrophysics Data System (ADS)

Kemper, Thomas; Gueguen, Lionel; Soille, Pierre

2012-06-01

The enumeration of the population remains a critical task in the management of refugee/IDP camps. Analysis of very high spatial resolution satellite data proofed to be an efficient and secure approach for the estimation of dwellings and the monitoring of the camp over time. In this paper we propose a new methodology for the automated extraction of features based on differential morphological decomposition segmentation for feature extraction and interactive training sample selection from the max-tree and min-tree structures. This feature extraction methodology is tested on a WorldView-2 scene of an IDP camp in Darfur Sudan. Special emphasis is given to the additional available bands of the WorldView-2 sensor. The results obtained show that the interactive image information tool is performing very well by tuning the feature extraction to the local conditions. The analysis of different spectral subsets shows that it is possible to obtain good results already with an RGB combination, but by increasing the number of spectral bands the detection of dwellings becomes more accurate. Best results were obtained using all eight bands of WorldView-2 satellite.

Processing techniques for software based SAR processors

NASA Technical Reports Server (NTRS)

Leung, K.; Wu, C.

1983-01-01

Software SAR processing techniques defined to treat Shuttle Imaging Radar-B (SIR-B) data are reviewed. The algorithms are devised for the data processing procedure selection, SAR correlation function implementation, multiple array processors utilization, cornerturning, variable reference length azimuth processing, and range migration handling. The Interim Digital Processor (IDP) originally implemented for handling Seasat SAR data has been adapted for the SIR-B, and offers a resolution of 100 km using a processing procedure based on the Fast Fourier Transformation fast correlation approach. Peculiarities of the Seasat SAR data processing requirements are reviewed, along with modifications introduced for the SIR-B. An Advanced Digital SAR Processor (ADSP) is under development for use with the SIR-B in the 1986 time frame as an upgrade for the IDP, which will be in service in 1984-5.

Identification of pixels with stray light and cloud shadow contaminations in the satellite ocean color data processing.

PubMed

Jiang, Lide; Wang, Menghua

2013-09-20

A new flag/masking scheme has been developed for identifying stray light and cloud shadow pixels that significantly impact the quality of satellite-derived ocean color products. Various case studies have been carried out to evaluate the performance of the new cloud contamination flag/masking scheme on ocean color products derived from the Visible Infrared Imaging Radiometer Suite (VIIRS) onboard the Suomi National Polar-orbiting Partnership (SNPP). These include direct visual assessments, detailed quantitative case studies, objective statistic analyses, and global image examinations and comparisons. The National Oceanic and Atmospheric Administration (NOAA) Multisensor Level-1 to Level-2 (NOAA-MSL12) ocean color data processing system has been used in the study. The new stray light and cloud shadow identification method has been shown to outperform the current stray light flag in both valid data coverage and data quality of satellite-derived ocean color products. In addition, some cloud-related flags from the official VIIRS-SNPP data processing software, i.e., the Interface Data Processing System (IDPS), have been assessed. Although the data quality with the IDPS flags is comparable to that of the new flag implemented in the NOAA-MSL12 ocean color data processing system, the valid data coverage from the IDPS is significantly less than that from the NOAA-MSL12 using the new stray light and cloud shadow flag method. Thus, the IDPS flag/masking algorithms need to be refined and modified to reduce the pixel loss, e.g., the proposed new cloud contamination flag/masking can be implemented in IDPS VIIRS ocean color data processing.

The National Polar-orbiting Operational Environmental Satellite System

NASA Astrophysics Data System (ADS)

Bloom, H.

The tri-agency Integrated Program Office (IPO) is responsible for managing the development of the National Polar-orbiting Operational Environmental Satellite System (NPOESS). NPOESS will replace the current military and civilian operational polar-orbiting ``weather'' satellites. The Northrop Grumman Space Technology - Raytheon team was competitively selected in 2002 as the Acquisition and Operations contractor team to develop, integrate, deploy, and operate NPOESS satellites to meet the tri-agency user requirements for NPOESS over the 10-year (2009-2018) operational life of the program. Beginning in 2009, NPOESS spacecraft will be launched into three orbital planes to provide significantly improved operational capabilities and benefits to satisfy critical civil and national security requirements for space-based, remotely sensed environmental data. With the development of NPOESS, we are evolving operational ``weather'' satellites into integrated environmental observing systems by expanding our capabilities to observe, assess, and predict the total Earth system - atmosphere, ocean, land, and the space environment. In recent years, the operational weather forecasting and climate science communities have levied more rigorous requirements on space-based observations of the Earth's system that have significantly increased demands on performance of the instruments, spacecraft, and ground systems required to deliver NPOESS data, products, and information to end users. The ``end-to-end'' system consists of: the spacecraft; instruments and sensors on the spacecraft; launch support capabilities; the command, control, communications, and data routing infrastructure; and data processing hardware and software. NPOESS will observe significantly more phenomena simultaneously from space than its operational predecessors. NPOESS is expected to deliver large volumes of more accurate measurements at higher spatial (horizontal and vertical) and temporal resolution at much higher data

Image processing and products for the Magellan mission to Venus

NASA Technical Reports Server (NTRS)

Clark, Jerry; Alexander, Doug; Andres, Paul; Lewicki, Scott; Mcauley, Myche

1992-01-01

The Magellan mission to Venus is providing planetary scientists with massive amounts of new data about the surface geology of Venus. Digital image processing is an integral part of the ground data system that provides data products to the investigators. The mosaicking of synthetic aperture radar (SAR) image data from the spacecraft is being performed at JPL's Multimission Image Processing Laboratory (MIPL). MIPL hosts and supports the Image Data Processing Subsystem (IDPS), which was developed in a VAXcluster environment of hardware and software that includes optical disk jukeboxes and the TAE-VICAR (Transportable Applications Executive-Video Image Communication and Retrieval) system. The IDPS is being used by processing analysts of the Image Data Processing Team to produce the Magellan image data products. Various aspects of the image processing procedure are discussed.

IDP-ASE: haplotyping and quantifying allele-specific expression at the gene and gene isoform level by hybrid sequencing

PubMed Central

Deonovic, Benjamin; Wang, Yunhao; Weirather, Jason; Wang, Xiu-Jie; Au, Kin Fai

2017-01-01

Abstract Allele-specific expression (ASE) is a fundamental problem in studying gene regulation and diploid transcriptome profiles, with two key challenges: (i) haplotyping and (ii) estimation of ASE at the gene isoform level. Existing ASE analysis methods are limited by a dependence on haplotyping from laborious experiments or extra genome/family trio data. In addition, there is a lack of methods for gene isoform level ASE analysis. We developed a tool, IDP-ASE, for full ASE analysis. By innovative integration of Third Generation Sequencing (TGS) long reads with Second Generation Sequencing (SGS) short reads, the accuracy of haplotyping and ASE quantification at the gene and gene isoform level was greatly improved as demonstrated by the gold standard data GM12878 data and semi-simulation data. In addition to methodology development, applications of IDP-ASE to human embryonic stem cells and breast cancer cells indicate that the imbalance of ASE and non-uniformity of gene isoform ASE is widespread, including tumorigenesis relevant genes and pluripotency markers. These results show that gene isoform expression and allele-specific expression cooperate to provide high diversity and complexity of gene regulation and expression, highlighting the importance of studying ASE at the gene isoform level. Our study provides a robust bioinformatics solution to understand ASE using RNA sequencing data only. PMID:27899656

Assessment of the NPOESS/VIIRS Nighttime Infrared Cloud Optical Properties Algorithms

NASA Astrophysics Data System (ADS)

Wong, E.; Ou, S. C.

2008-12-01

In this paper we will describe two NPOESS VIIRS IR algorithms used to retrieve microphysical properties for water and ice clouds during nighttime conditions. Both algorithms employ four VIIRS IR channels: M12 (3.7 μm), M14 (8.55 μm), M15 (10.7 μm) and M16 (12 μm). The physical basis for the two algorithms is similar in that while the Cloud Top Temperature (CTT) is derived from M14 and M16 for ice clouds the Cloud Optical Thickness (COT) and Cloud Effective Particle Size (CEPS) are derived from M12 and M15. The two algorithms depart in the different radiative transfer parameterization equations used for ice and water clouds. Both the VIIRS nighttime IR algorithms and the CERES split-window method employ the 3.7 μm and 10.7 μm bands for cloud optical properties retrievals, apparently based on similar physical principles but with different implementations. It is reasonable to expect that the VIIRS and CERES IR algorithms produce comparable performance and similar limitations. To demonstrate the VIIRS nighttime IR algorithm performance, we will select a number of test cases using NASA MODIS L1b radiance products as proxy input data for VIIRS. The VIIRS retrieved COT and CEPS will then be compared to cloud products available from the MODIS, NASA CALIPSO, CloudSat and CERES sensors. For the MODIS product, the nighttime cloud emissivity will serve as an indirect comparison to VIIRS COT. For the CALIPSO and CloudSat products, the layered COT will be used for direct comparison. Finally, the CERES products will provide direct comparison with COT as well as CEPS. This study can only provide a qualitative assessment of the VIIRS IR algorithms due to the large uncertainties in these cloud products.

Heterogeneous access and processing of EO-Data on a Cloud based Infrastructure delivering operational Products

NASA Astrophysics Data System (ADS)

Niggemann, F.; Appel, F.; Bach, H.; de la Mar, J.; Schirpke, B.; Dutting, K.; Rucker, G.; Leimbach, D.

2015-04-01

To address the challenges of effective data handling faced by Small and Medium Sized Enterprises (SMEs) a cloud-based infrastructure for accessing and processing of Earth Observation(EO)-data has been developed within the project APPS4GMES(www.apps4gmes.de). To gain homogenous multi mission data access an Input Data Portal (IDP) been implemented on this infrastructure. The IDP consists of an Open Geospatial Consortium (OGC) conformant catalogue, a consolidation module for format conversion and an OGC-conformant ordering framework. Metadata of various EO-sources and with different standards is harvested and transferred to an OGC conformant Earth Observation Product standard and inserted into the catalogue by a Metadata Harvester. The IDP can be accessed for search and ordering of the harvested datasets by the services implemented on the cloud infrastructure. Different land-surface services have been realised by the project partners, using the implemented IDP and cloud infrastructure. Results of these are customer ready products, as well as pre-products (e.g. atmospheric corrected EO data), serving as a basis for other services. Within the IDP an automated access to ESA's Sentinel-1 Scientific Data Hub has been implemented. Searching and downloading of the SAR data can be performed in an automated way. With the implementation of the Sentinel-1 Toolbox and own software, for processing of the datasets for further use, for example for Vista's snow monitoring, delivering input for the flood forecast services, can also be performed in an automated way. For performance tests of the cloud environment a sophisticated model based atmospheric correction and pre-classification service has been implemented. Tests conducted an automated synchronised processing of one entire Landsat 8 (LS-8) coverage for Germany and performance comparisons to standard desktop systems. Results of these tests, showing a performance improvement by the factor of six, proved the high flexibility and

The prevalence of HBV infection in the cohort of IDPs of war against terrorism in Malakand Division of Northern Pakistan

PubMed Central

2011-01-01

Background Hepatitis B is an important public health problem in the Pakistani population and is the major cause of chronic hepatitis, cirrhosis, fibrosis and hepatocellular carcinoma. High prevalence of HBV infections has been observed especially in areas of low economic status. In spite of effective immunization programs, no significant change has been observed in the epidemiology of HBV in the rural areas of Pakistan (~67.5% of the total population) mainly due to lack of interest from government authorities and poor hygienic measures. The current study was aimed at estimating the prevalence and risk factors associated with HBV infection within internally displaced persons (IDPs) due to war against terrorism in the Malakand Division of Northern Pakistan. Methods Blood samples from 950 IDPs suspected with HBV infection (including both males and females) were collected and processed with commercial ELISA kits for HBsAg, Anti HBs, HBeAg, Anti HBe antibodies. The samples positive by ELISA were confirmed for HBV DNA by real-time PCR analysis. Results The overall prevalence of HBV observed was 21.05% of which 78.5% were males and 21.5% were females. Most confirmed HBV patients belong to the Malakand and Dir (lower) district. High-risk of infection was found in the older subjects 29.13% (46-60 years), while a lower incidence (11.97%) was observed in children aged <15 years. Lack of awareness, socioecomic conditions, sexual activities and sharing of razor blades, syringes and tattooing needles were the most common risk factors of HBV infection observed during the cohort of patients. Conclusion The present study, revealed for the first time a high degree of prevalence of HBV infection in rural areas of Northern Pakistan. The noticed prevalence is gender- and age-dependent that might be due to their high exposures to the common risk factors. To avoid the transmission of HBV infection proper awareness about the possible risk factors and extension of immunization to the rural

Evidence for Changes in 81PIWild 2 Organic Matter Since Collection and Comparison of 82PIWild 2 and IDP Organic Matter to Access the Thermal Effects of Aerogel Capture

NASA Technical Reports Server (NTRS)

Wirick, S.; Flynn, G. J.; Keller, L.; Messenger, Nakamura; Sandford, S. A.; Zolensky, M. E.; Peltzer, C.; Jacobsen, C.

2009-01-01

NASA s Stardust spacecraft collected cometary material during its passage through the dust coma of comet 81P/Wild 2 on January 2nd, 2004 and delivered this material to Earth on January 15th 2006. The first fragment we analyzed during the preliminary examination was partially vaporized by the X-ray beam. The carbonaceous material that survived was re-analysis approx.2 months later and the carbon spectrum for this material had significantly changed from what we first observed.. We have observed similar changes to the carbonaceous matter in some interplanetary dust particles ( IDPs). Some of the 81P/Wild 2 organic matter volatilized upon impact with the aerogel as observed using IR spectroscopy where IR spectra were collected several mms away from sample tracks [1]. The time-temperature profile experienced by any particular 81P/Wild 2 grain during aerogel capture is not known, although Brownlee, et al. suggest that fine-grained materials, <1 micron in size, fragmented and then partially vaporized during collection, while particles much larger then 1 micron in size were captured intact [2]. Nearly all organic matter is subject to thermal alteration. To assess the heating and alteration experienced by the 81P/Wild 2 organic matter during capture we are comparing 81P/Wild2 organic matter with IDP organic matter where we have evidence of heating in the IDP [3,4].

Comparison of Nickel XANES Spectra and Elemental Maps from a Ureilite, a LL3.8 Ordinary Chondrite, Two Carbonaceous Chondrites and Two Large Cluster IDPs

NASA Technical Reports Server (NTRS)

Wirick, S.; Flynn, G. J.; Sutton, S.; Zolensky, M. E.

2014-01-01

Nickel in the extraterrestrial world is commonly found in both Fe-Ni sulfide and Fe-Ni met-al forms [1] and in the pure metal state in the interior of iron meteorites where it is not easily oxidized. Ni is also found in olivine, pyroxene and glasses and in some melts the partitioning of Ni between the olivines and glass is controlled by the amount of S in the melt [2]. Its most common valence state is Ni(2+) but Ni also occurs as Ni(0), Ni(+), and Ni(3+) and rarely as Ni(2-), Ni(1-) and Ni(4+) [3]. It's valence state in olivines is Ni(2+) in octa-hedral coordination on the M1 site and rarely on the M2 site.[4]. The chemical sensitivity of X-ray absorp-tion near-edge structure (XANES) spectroscopy is well established and can be used to determine not only va-lence states but also coordination sites [5]. We report here Ni XANES spectroscopy and elemental maps collected from 2 carbonaceous chondrites, 2 large clus-ter IDPs, 1 ureilite and 1 LL3 orginary chondrite.Using XANES it may be possible to find a common trait in the large cluster IDPs that will also be found in mete-orite samples.

Workshop on the Analysis of Interplanetary Dust Particles

NASA Technical Reports Server (NTRS)

Zolensky, Michael E. (Editor)

1994-01-01

Great progress has been made in the analysis of interplanetary dust particles (IDP's) over the past few years. This workshop provided a forum for the discussion of the following topics: observation and modeling of dust in the solar system, mineralogy and petrography of IDP's, processing of IDP's in the solar system and terrestrial atmosphere, comparison of IDP's to meteorites and micrometeorites, composition of IDP's, classification, and collection of IDP's.

Putting PhDs to work: career planning for today's scientist.

PubMed

Hobin, Jennifer A; Clifford, Philip S; Dunn, Ben M; Rich, Susan; Justement, Louis B

2014-01-01

Individual development plans (IDPs) have been promoted nationally as a tool to help research trainees explore career opportunities and set career goals. Despite the interest in IDPs from a policy perspective, there is little information about how they have been used. The authors examined IDP awareness and use, the benefits of creating an IDP, and ways to facilitate its use by administering a survey to current or former postdoctoral researchers via the National Postdoctoral Association (NPA) and University of Alabama at Birmingham email lists; individuals belonging to Federation of American Societies for Experimental Biology member societies who mentored postdocs; and postdoctoral administrators at member institutions of the Association of American Medical Colleges and the NPA. Although most postdoctoral administrators (>80%) were familiar with IDPs, less than 50% of postdocs and only 20% of mentors were aware of IDPs. For those postdocs and mentors who reported creating an IDP, the process helped postdocs to identify the skills and abilities necessary for career success and facilitated communication between postdocs and their mentors. Despite the fact that creating an IDP benefits postdocs and mentors, IDP use will likely remain low unless institutions and research mentors encourage trainees to engage in this process.

Putting PhDs to Work: Career Planning for Today's Scientist

PubMed Central

Hobin, Jennifer A.; Clifford, Philip S.; Dunn, Ben M.; Rich, Susan; Justement, Louis B.

2014-01-01

Individual development plans (IDPs) have been promoted nationally as a tool to help research trainees explore career opportunities and set career goals. Despite the interest in IDPs from a policy perspective, there is little information about how they have been used. The authors examined IDP awareness and use, the benefits of creating an IDP, and ways to facilitate its use by administering a survey to current or former postdoctoral researchers via the National Postdoctoral Association (NPA) and University of Alabama at Birmingham email lists; individuals belonging to Federation of American Societies for Experimental Biology member societies who mentored postdocs; and postdoctoral administrators at member institutions of the Association of American Medical Colleges and the NPA. Although most postdoctoral administrators (>80%) were familiar with IDPs, less than 50% of postdocs and only 20% of mentors were aware of IDPs. For those postdocs and mentors who reported creating an IDP, the process helped postdocs to identify the skills and abilities necessary for career success and facilitated communication between postdocs and their mentors. Despite the fact that creating an IDP benefits postdocs and mentors, IDP use will likely remain low unless institutions and research mentors encourage trainees to engage in this process. PMID:24591503

The Effects of Differentiated Instruction on the Literacy Process of Learners with Interrupted Schooling

ERIC Educational Resources Information Center

Niño Santisteban, Liliana

2014-01-01

This research study analyzes the literacy and foreign langauge processes of learners in the "Procesos Básicos" Program. The participants were 15 Spanish-speaking children and young adolescents, whose highest level of education was first grade. Eight of the 15 children were Internally Displaced Persons (IDPs), and the others were affected…

The National Polar-orbiting Operational Environmental Satellite System

NASA Astrophysics Data System (ADS)

Hoffman, C. W.; Mango, S.; Schneider, S.; Duda, J.; Haas, J.; Bloom, H.

2005-12-01

Over the last decade, the tri-agency Integrated Program Office (IPO), comprised of the National Oceanic and Atmospheric Administration (NOAA), the Department of Defense (DoD), and the National Aeronautics and Space Administration (NASA), has been managing the development of the National Polar-orbiting Operational Environmental Satellite System (NPOESS). Once operational later this decade, NPOESS will replace NOAA's Polar-orbiting Operational Environmental Satellites (POES) and DoD's Defense Meteorological Satellite Program (DMSP) systems. The IPO, through its Acquisition and Operations contractor, Northrop Grumman, will launch NPOESS spacecraft into three orbital planes to provide a single, national system capable of satisfying both civil and national security requirements for space-based, remotely sensed environmental data. With the development of NPOESS, we are evolving the existing 'weather' satellites into integrated environmental observing systems by expanding our capabilities to observe, assess, and predict the total Earth system - ocean, atmosphere, land, and the space environment. NPOESS will transform today's short-term, space-based ocean research missions into a sustained, operational ocean remote sensing observation program. Ocean measurements comprise one-fourth of the 55 user-validated requirements for geophysical measurements that will be made by NPOESS sensors. In 1997, the IPO initiated a robust sensor risk reduction effort for early development of the critical sensor suites and algorithms necessary to support NPOESS. In 2001, preliminary design efforts were completed for the last of five critical imaging/sounding instruments for NPOESS. Ocean requirements have directly and substantially 'driven' the design of three NPOESS sensors: the Visible/Infrared Imager Radiometer Suite (VIIRS); the Conical-scanning Microwave Imager/Sounder (CMIS); and the Altimeter. With these instruments, NPOESS will deliver higher resolution (spatial and temporal) and more

IDP camp evolvement analysis in Darfur using VHSR optical satellite image time series and scientific visualization on virtual globes

NASA Astrophysics Data System (ADS)

Tiede, Dirk; Lang, Stefan

2010-11-01

In this paper we focus on the application of transferable, object-based image analysis algorithms for dwelling extraction in a camp for internally displaced people (IDP) in Darfur, Sudan along with innovative means for scientific visualisation of the results. Three very high spatial resolution satellite images (QuickBird: 2002, 2004, 2008) were used for: (1) extracting different types of dwellings and (2) calculating and visualizing added-value products such as dwelling density and camp structure. The results were visualized on virtual globes (Google Earth and ArcGIS Explorer) revealing the analysis results (analytical 3D views,) transformed into the third dimension (z-value). Data formats depend on virtual globe software including KML/KMZ (keyhole mark-up language) and ESRI 3D shapefiles streamed as ArcGIS Server-based globe service. In addition, means for improving overall performance of automated dwelling structures using grid computing techniques are discussed using examples from a similar study.

Assemblage of Presolar Materials and Early Solar System Condensates in Chondritic Porous Interplanetary Dust Particles

NASA Technical Reports Server (NTRS)

Nguyen, A. N.; Nakamura-Messenger, K.; Messenger, S.; Keller, L. P.; Kloeck, W.

2015-01-01

Anhydrous chondritic porous inter-planetary dust particles (CP IDPs) contain an assortment of highly primitive solar system components, molecular cloud matter, and presolar grains. These IDPs have largely escaped parent body processing that has affected meteorites, advocating cometary origins. Though the stardust abundance in CP IDPs is generally greater than in primitive meteorites, it can vary widely among individual CP IDPs. The average abundance of silicate stardust among isotopically primitive IDPs is approx. 375 ppm while some have extreme abundances up to approx. 1.5%. H and N isotopic anomalies are common in CP IDPs and the carrier of these anomalies has been traced to organic matter that has experienced chemical reactions in cold molecular clouds or the outer protosolar disk. Significant variations in these anomalies may reflect different degrees of nebular processing. Refractory inclusions are commonly observed in carbonaceous chondrites. These inclusions are among the first solar system condensates and display 16O-rich isotopic compositions. Refractory grains have also been observed in the comet 81P/Wild-2 samples re-turned from the Stardust Mission and in CP IDPs, but they occur with much less frequency. Here we conduct coordinated mineralogical and isotopic analyses of CP IDPs that were characterized for their bulk chemistry by to study the distribution of primitive components and the degree of nebular alteration incurred.

Validation Test Report for the Automated Optical Processing System (AOPS) Version 4.12

DTIC Science & Technology

2015-09-03

the Geostationary Ocean Color Imager (GOCI) sensor, aboard the Communication Ocean and Meteorological Satellite (COMS) satellite. Additionally, this...this capability works in conjunction with AOPS • Improvements to the AOPS mosaicking capability • Prepare the NRT Geostationary Ocean Color Imager...Warfare (EXW) Geostationary Ocean Color Imager (GOCI) Gulf of Mexico (GOM) Hierarchical Data Format (HDF) Integrated Data Processing System (IDPS

A Fragment-Based Method of Creating Small-Molecule Libraries to Target the Aggregation of Intrinsically Disordered Proteins.

PubMed

Joshi, Priyanka; Chia, Sean; Habchi, Johnny; Knowles, Tuomas P J; Dobson, Christopher M; Vendruscolo, Michele

2016-03-14

The aggregation process of intrinsically disordered proteins (IDPs) has been associated with a wide range of neurodegenerative disorders, including Alzheimer's and Parkinson's diseases. Currently, however, no drug in clinical use targets IDP aggregation. To facilitate drug discovery programs in this important and challenging area, we describe a fragment-based approach of generating small-molecule libraries that target specific IDPs. The method is based on the use of molecular fragments extracted from compounds reported in the literature to inhibit of the aggregation of IDPs. These fragments are used to screen existing large generic libraries of small molecules to form smaller libraries specific for given IDPs. We illustrate this approach by describing three distinct small-molecule libraries to target, Aβ, tau, and α-synuclein, which are three IDPs implicated in Alzheimer's and Parkinson's diseases. The strategy described here offers novel opportunities for the identification of effective molecular scaffolds for drug discovery for neurodegenerative disorders and to provide insights into the mechanism of small-molecule binding to IDPs.

Principal components - Petrology and chemistry of polyphase units in chondritic porous interplanetary dust particles

NASA Astrophysics Data System (ADS)

Rietmeijer, Frans J. M.

1997-03-01

Chondritic porous (CP) interplanetary dust particles (IDPs) can be described as 'cosmic sediments'. It should be possible to recognize in these IDPs the 4500 Myrs old solar nebula dusts. The studies of unaltered chondritic IDPs show that their matrices are a mixture of three different principal components (PCs) that also describe variable C/Si ratios of chondritic IDPs. Among others, PCs include polyphase units (PUs) that are amorphous to holocrystalline, both ultrafine- and coarse-grained, ferromagnesiosilica(te) materials with minor Al and Ca. The properties of PCs and their alteration products define the physical and chemical processes that produced and altered these components. PCs are also cornerstones of IDP classification. For example, the bulk composition of ultrafine-grained PCs can be reconstructed using the 'butterfly method' and also allows an evaluation of the metamorphic signatures, (e.g., dynamic pyrometamorphism), in chondritic IDPs.

Incorporating prototyping and iteration into intervention development: a case study of a dining hall-based intervention.

PubMed

McClain, Arianna D; Hekler, Eric B; Gardner, Christopher D

2013-01-01

Previous research from the fields of computer science and engineering highlight the importance of an iterative design process (IDP) to create more creative and effective solutions. This study describes IDP as a new method for developing health behavior interventions and evaluates the effectiveness of a dining hall-based intervention developed using IDP on college students' eating behavior and values. participants were 458 students (52.6% female, age = 19.6 ± 1.5 years [M ± SD]). The intervention was developed via an IDP parallel process. A cluster-randomized controlled study compared differences in eating behavior among students in 4 university dining halls (2 intervention, 2 control). The final intervention was a multicomponent, point-of-selection marketing campaign. Students in the intervention dining halls consumed significantly less junk food and high-fat meat and increased their perceived importance of eating a healthful diet relative to the control group. IDP may be valuable for the development of behavior change interventions.

Challenges to polycentric governance of an international development project tackling land degradation in Swaziland.

PubMed

Orchard, Steven E; Stringer, Lindsay C

2016-11-01

To effectively address the drivers and impacts of land degradation requires polycentric governance systems that facilitate international development projects (IDPs). This paper analyses an IDP aiming to reduce land degradation in Swaziland. A longitudinal-style qualitative approach draws on repeat household surveys, semi-structured interviews and focus groups. We aim to identify the changes that have taken place since the departure of the IDP funders, and the subsequent dynamics between stakeholders. We: (1) chart the evolution of the institutional structures and processes of the IDP; and (2) assess community perceptions of IDP outcomes. Lack of meaningful participation at various stages of the PMC caused the project to lose momentum following the departure of the funders. We discuss these findings in relation to a polycentric approach, and identify how multi-stakeholder IDP can be facilitated as part of wider polycentric governance approaches to inform policies to combat land degradation within Swaziland and more widely.

Thin-sectioning and analysis of fine-grained meteoritic materials

NASA Technical Reports Server (NTRS)

Brooks, Donald A. (Editor); Bradley, John P.

1992-01-01

The overall theme of the work was the identification of the sources and formation/aggregation mechanisms of the various classes of interplanetary dust particles (IDP's) and to clarify the relationship between IDP's and conventional meteorites. IDP's are believed to be derived from a much broader range of parent bodies than conventional meteorites. Some of these parent bodies (e.g., comets) have escaped that post accretional processing that has affected the parent bodies of meteorites. Therefore, IDP's are likely to preserve a record of early solar system and possibly presolar grain forming reactions. Using analytical electron microscopy (AEM) and more recently micro-infrared (IR) microspectroscopy to examine ultramicrotomed thin sections, we have addressed the questions of IDP formation mechanisms, sources, and their relationship to conventional meteorites. The following sections describe specific findings resulting from these studies.

Analytical electron microscopy of fine-grained phases in primitive interplanetary dust particles and carbonaceous chondrites

NASA Technical Reports Server (NTRS)

Mackinnon, Ian D. R.; Rietmeijer, Frans J. M.; Mckay, David S.

1987-01-01

In order to describe the total mineralogical diversity within primitive extraterrestrial materials, individual interplanetary dust particles (IDPs) collected from the stratosphere as part of the JSC Cosmic Dust Curatorial Program were analyzed using a variety of AEM techniques. Identification of over 250 individual grains within one chondritic porous (CP) IDP shows that most phases could be formed by low temperature processes and that heating of the IDP during atmospheric entry is minimal and less than 600 C. In a review of the mineralogy of IDPs, it was suggested that the occurrence of other silicates such as enstatite whiskers is consistent with the formation in an early turbulent period of the solar nebula. Experimental confirmation of fundamental chemical and physical processes in a stellar environment, such as vapor phase condensation, nucleation, and growth by annealing, is an important aspect of astrophysical models for the evolution of the Solar System. A detailed comparison of chondritic IDP and carbonaceous chondrite mineralogies shows significant differences between the types of silicate minerals as well as the predominant oxides.

Discovery of Brownleeite: a New Manganese Silicide Mineral in an Interplanetary Dust Particle

NASA Technical Reports Server (NTRS)

Keller, Lindsay P.; Nakamura-Messenger, Keiko; Clemett, Simon J.; Messenger, Scott; Jones, John H.; Palma, Russell L.; Pepin, Robert O.; Klock, Wolfgang; Zolensky, Michael E.; Tatsuoka, Hirokazu

2011-01-01

The Earth accretes approximately 40,000 tons of cosmic dust annually, originating mainly from the disintegration of comets and collisions among asteroids. This cosmic dust, also known as interplanetary dust particles (IDPs), is a subject of intense interest since it is made of the original building blocks of our Solar System. Although the specific parent bodies of IDPs are unknown, the anhydrous chondritic-porous IDPs (CP-IDPs) subset has been potentially linked to a cometary source. The CP-IDPs are extremely primitive materials based on their unequilibrated mineralogy, C-rich chemistry, and anomalous isotopic signatures. In particular, some CP-IDPs escaped the thermal, aqueous and impact shock processing that has modified or destroyed the original mineralogy of meteorites. Thus, the CP-IDPs represent some of the most primitive solar system materials available for laboratory study. Most CP-IDPs are comprised of minerals that are common on Earth. However, in the course of an examination of one of the CP-IDPs, we encountered three sub-micrometer sized grains of manganese silicide (MnSi), a phase that has heretofore not been found in nature. In the seminar, we would like to focus on IDP studies and this manganese silicide phase that has been approved as the first new mineral identified from a comet by the International Mineralogical Association (IMA) in 2008. The mineral is named in honour of Donald E. Brownlee, an American astronomer and a founder of the field of cosmic dust research who is the principal investigator of the NASA Stardust Mission that collected dust samples from Comet 81P/Wild-2 and returned them to Earth. Much of our current view and understanding of the early solar system would not exist without the pioneering work of professor Don Brownlee in the study of IDPs.

Carbon petrology in cometary dust

NASA Technical Reports Server (NTRS)

Rietmeijer, Frans J. M.

1992-01-01

Chondritic porous (CP) interplanetary dust particles (IDP's) are collected in the Earth's stratosphere. There exists an extensive database on major and minor element chemistry, stable isotopes, noble gas abundances and mineralogy of many CP IDP's, as well as infrared and Raman spectroscopic properties. For details on the mineralogy, chemistry and physical properties of IDP's, I refer to the reviews by Mackinnon and Rietmeijer (1987), Bradley et al. (1988) and Sandford (1987). Texture, mineralogy (Mackinnon and Rietmeijer, 1987) and chemistry (Schramm et al., 1989; Flynn and Sutton, 1991) support the notion that CP IDP's are a unique group of ultrafine-grained extraterrestiral materials that are distinct from any known meteorite class. Their fluffy, or porous, morphology suggests that CP IDP's probably endured minimal alteration by protoplanetary processes since their formation. It is generally accepted that CP IDP's are solid debris from short-period comets. The evidence is mostly circumstantial but this notion gained significant support based on the comet Halley dust data (Brownlee, 1990). In this paper, I will accept that CP IDP's are indeed cometary dust. The C/Si ratio in CP IDP's is 3.3 times higher than in CI carbonaceous chondrites (Schramm et al. 1989). The intraparticle carbon distribution is heteorogeneous (Rietmeijer and McKay, 1986). Carbon occurs both in oxidized and reduced forms. Analytical electron microscope (AEM) and Raman spectroscopic analyses have shown the presence of several carbon forms in CP IDP's but the data are scattered in the literature. Carbons in cometary CP IDP's are among the most pristine Solar System carbons available for laboratory study. Similar to a recently developed petrological model for the diversity of layer silicates in CP IDP's (Zolensky, 1991) that is useful to constrain in situ aqueous alteration in comets (Rietmeijer and Mackinnon, 1987a), I here present the first effort to develop a petrological concept of carbons

Local constructions of gender-based violence amongst IDPs in northern Uganda: analysis of archival data collected using a gender- and age-segmented participatory ranking methodology.

PubMed

Ager, Alastair; Bancroft, Carolyn; Berger, Elizabeth; Stark, Lindsay

2018-01-01

Gender-based violence (GBV) is a significant problem in conflict-affected settings. Understanding local constructions of such violence is crucial to developing preventive and responsive interventions to address this issue. This study reports on a secondary analysis of archived data collected as part of formative qualitative work - using a group participatory ranking methodology (PRM) - informing research on the prevalence of GBV amongst IDPs in northern Uganda in 2006. Sixty-four PRM group discussions were held with women, with men, with girls (aged 14 to 18 years), and with boys (aged 14 to 18 years) selected on a randomized basis across four internally displaced persons (IDP) camps in Lira District. Discussions elicited problems facing women in the camps, and - through structured participatory methods - consensus ranking of their importance and narrative accounts explaining these judgments. Amongst forms of GBV faced by women, rape was ranked as the greatest concern amongst participants (with a mean problem rank of 3.4), followed by marital rape (mean problem rank of 4.5) and intimate partner violence (mean problem rank of 4.9). Girls ranked all forms of GBV as higher priority concerns than other participants. Discussions indicated that these forms of GBV were generally considered normalized within the camp. Gender roles and power, economic deprivation, and physical and social characteristics of the camp setting emerged as key explanatory factors in accounts of GBV prevalence, although these played out in different ways with respect to differing forms of violence. All groups acknowledged GBV to represent a significant threat - among other major concerns such as transportation, water, shelter, food and security - for women residing in the camps. Given evidence of the significantly higher risk in the camp of intimate partner violence and marital rape, the relative prominence of the issue of rape in all rankings suggests normalization of violence within the home

Continued Investigations of the Accretion History of Extraterrestrial Matter over Geologic Time

NASA Technical Reports Server (NTRS)

Farley, Kenneth

2001-01-01

This grant supported our ongoing project to characterize the accretion rate of interplanetary dust particles (IDPs) to Earth over geologic time using He-3 as a tracer. IDPs are derived from collisions in the asteroid belt and from disaggregation of active comets. Owing to their small size (few to few hundred micrometers diameter) these particles spiral into the sun under Poynting-Robertson drag typically in less than a few tens of kyrs. Thus IDPs must be continually resupplied to the zodiacal cloud, and because the processes of IDP production are likely to be sporadic, time variation in the IDP accretion rate to Earth is likely to be time-varying. For example, major asteroidal collisions and comet showers should greatly enhance the IDP accretion rate. Our ultimate objective (still ongoing) is to document this time variance so as to better understand the history of the solar system, the source of IDPs accreting to Earth, and the details of the mechanism by which particles are captured by Earth. To document variations in IDP accretion rate through time we use He-3 as a tracer. This isotope is in extremely low abundance in terrestrial matter, but IDPs have very high concentrations of He-3 from implantation of solar wind ions. By measuring He-3 in seafloor sediments, we can estimate the IDP accretion rate for at least the last few hundred Myrs. Under an earlier NASA grant we identified the existence of a large increase in He-3 flux in the Late Eocene (35 Myr ago), coincident with the two largest impact craters of the Cenozoic Era. The simplest interpretation of this observation is the occurrence of a shower of long period comets at that time, simultaneously increasing the impact cratering probability and accretion rate of IDPs to Earth (Farley et al., 1998). Comet showers produced by stellar perturbation of the Oort cloud should be fairly common in the geologic record, so this is not an unreasonable interpretation of our observations.

Incorporating Prototyping and Iteration into Intervention Development: A Case Study of a Dining Hall-Based Intervention

ERIC Educational Resources Information Center

McClain, Arianna D.; Hekler, Eric B.; Gardner, Christopher D.

2013-01-01

Background: Previous research from the fields of computer science and engineering highlight the importance of an iterative design process (IDP) to create more creative and effective solutions. Objective: This study describes IDP as a new method for developing health behavior interventions and evaluates the effectiveness of a dining hall--based…

Oxygen isotopic composition of chondritic interplanetary dust particles: A genetic link between carbonaceous chondrites and comets

NASA Astrophysics Data System (ADS)

Aléon, J.; Engrand, C.; Leshin, L. A.; McKeegan, K. D.

2009-08-01

Oxygen isotopes were measured in four chondritic hydrated interplanetary dust particles (IDPs) and five chondritic anhydrous IDPs including two GEMS-rich particles (Glass embedded with metal and sulfides) by a combination of high precision and high lateral resolution ion microprobe techniques. All IDPs have isotopic compositions tightly clustered around that of solar system planetary materials. Hydrated IDPs have mass-fractionated oxygen isotopic compositions similar to those of CI and CM carbonaceous chondrites, consistent with hydration of initially anhydrous protosolar dust. Anhydrous IDPs have small 16O excesses and depletions similar to those of carbonaceous chondrites, the largest 16O variations being hosted by the two GEMS-rich IDPs. Coarse-grained forsteritic olivine and enstatite in anhydrous IDPs are isotopically similar to their counterparts in comet Wild 2 and in chondrules suggesting a high temperature inner solar system origin. The small variations in the 16O content of GEMS-rich IDPs suggest that most GEMS either do not preserve a record of interstellar processes or the initial interstellar dust is not 16O-rich as expected by self-shielding models, although a larger dataset is required to verify these conclusions. Together with other chemical and mineralogical indicators, O isotopes show that the parent-bodies of carbonaceous chondrites, of chondritic IDPs, of most Antarctic micrometeorites, and comet Wild 2 belong to a single family of objects of carbonaceous chondrite chemical affinity as distinct from ordinary, enstatite, K- and R-chondrites. Comparison with astronomical observations thus suggests a chemical continuum of objects including main belt and outer solar system asteroids such as C-type, P-type and D-type asteroids, Trojans and Centaurs as well as short-period comets and other Kuiper Belt Objects.

Interstellar and Solar System Organic Matter Preserved in Interplanetary Dust

NASA Technical Reports Server (NTRS)

Messenger, Scott; Nakamura-Messenger, Keiko

2015-01-01

Interplanetary dust particles (IDPs) collected in the Earth's stratosphere derive from collisions among asteroids and by the disruption and outgassing of short-period comets. Chondritic porous (CP) IDPs are among the most primitive Solar System materials. CP-IDPs have been linked to cometary parent bodies by their mineralogy, textures, C-content, and dynamical histories. CP-IDPs are fragile, fine-grained (less than um) assemblages of anhydrous amorphous and crystalline silicates, oxides and sulfides bound together by abundant carbonaceous material. Ancient silicate, oxide, and SiC stardust grains exhibiting highly anomalous isotopic compositions are abundant in CP-IDPs, constituting 0.01 - 1 % of the mass of the particles. The organic matter in CP-IDPs is isotopically anomalous, with enrichments in D/H reaching 50x the terrestrial SMOW value and 15N/14N ratios up to 3x terrestrial standard compositions. These anomalies are indicative of low T (10-100 K) mass fractionation in cold molecular cloud or the outermost reaches of the protosolar disk. The organic matter shows distinct morphologies, including sub-um globules, bubbly textures, featureless, and with mineral inclusions. Infrared spectroscopy and mass spectrometry studies of organic matter in IDPs reveals diverse species including aliphatic and aromatic compounds. The organic matter with the highest isotopic anomalies appears to be richer in aliphatic compounds. These materials also bear similarities and differences with primitive, isotopically anomalous organic matter in carbonaceous chondrite meteorites. The diversity of the organic chemistry, morphology, and isotopic properties in IDPs and meteorites reflects variable preservation of interstellar/primordial components and Solar System processing. One unifying feature is the presence of sub-um isotopically anomalous organic globules among all primitive materials, including IDPs, meteorites, and comet Wild-2 samples returned by the Stardust mission.

Cross-Track Infrared Sounder Science Data Record Pre-launch Calibration and On-Orbit Validation Plans

NASA Astrophysics Data System (ADS)

Hagan, D. E.; Bingham, G. E.; Predina, J.; Gu, D.; Sabet-Peyman, F.; Wang, C.; de Amici, G.; Plonski, M.; Farrow, S. V.; Hohn, J.; Esplin, M.; Zavyalov, V.; Fish, C. S.; Glumb, R.; Wells, S.; Suwinski, L.; Strong, J.; Behrens, C.; Kilcoyne, H.; Feeley, J.; Kratz, G.; Tremblay, D. A.

2009-12-01

The Cross-Track Infrared Sounder (CrIS) together with the Advanced Technology Microwave Sounder will provide retrievals of atmospheric moisture and temperature profiles for the National Polar-orbiting Operational Environmental Satellite System (NPOESS). The NPOESS is the next generation of low Earth orbiting weather and climate satellites managed by the tri-agency Integrated Program Office, which includes the Department of Commerce, Department of Defense and the National Aeronautics and Space Administration. The CrIS is a Fourier-transform Michelson interferometer covering the spectral range of 3.9 to 15.4 microns (650 to 2550 wavenumbers) developed by ITT under contract to Northrop Grumman Aerospace Systems. The first deployment of the CrIS (Flight Model 1) is scheduled for 2010 on the NPOESS Preparatory Project (NPP) satellite, an early instrument risk reduction component of the NPOESS mission. The analysis and data results from comprehensive TVAC testing of the CrIS FM1 sensor demonstrate a very accurate radiometric and spectral calibration system. We describe instrument performance parameters, and the end-to-end plans and analysis tools for on-orbit verification of sensor characteristics and validation of the SDR radiance products.

Sequence Determinants of Compaction in Intrinsically Disordered Proteins

PubMed Central

Marsh, Joseph A.; Forman-Kay, Julie D.

2010-01-01

Abstract Intrinsically disordered proteins (IDPs), which lack folded structure and are disordered under nondenaturing conditions, have been shown to perform important functions in a large number of cellular processes. These proteins have interesting structural properties that deviate from the random-coil-like behavior exhibited by chemically denatured proteins. In particular, IDPs are often observed to exhibit significant compaction. In this study, we have analyzed the hydrodynamic radii of a number of IDPs to investigate the sequence determinants of this compaction. Net charge and proline content are observed to be strongly correlated with increased hydrodynamic radii, suggesting that these are the dominant contributors to compaction. Hydrophobicity and secondary structure, on the other hand, appear to have negligible effects on compaction, which implies that the determinants of structure in folded and intrinsically disordered proteins are profoundly different. Finally, we observe that polyhistidine tags seem to increase IDP compaction, which suggests that these tags have significant perturbing effects and thus should be removed before any structural characterizations of IDPs. Using the relationships observed in this analysis, we have developed a sequence-based predictor of hydrodynamic radius for IDPs that shows substantial improvement over a simple model based upon chain length alone. PMID:20483348

An Overview of the JPSS Ground Project Algorithm Integration Process

NASA Astrophysics Data System (ADS)

Vicente, G. A.; Williams, R.; Dorman, T. J.; Williamson, R. C.; Shaw, F. J.; Thomas, W. M.; Hung, L.; Griffin, A.; Meade, P.; Steadley, R. S.; Cember, R. P.

2015-12-01

The smooth transition, implementation and operationalization of scientific software's from the National Oceanic and Atmospheric Administration (NOAA) development teams to the Join Polar Satellite System (JPSS) Ground Segment requires a variety of experiences and expertise. This task has been accomplished by a dedicated group of scientist and engineers working in close collaboration with the NOAA Satellite and Information Services (NESDIS) Center for Satellite Applications and Research (STAR) science teams for the JPSS/Suomi-NPOES Preparatory Project (S-NPP) Advanced Technology Microwave Sounder (ATMS), Cross-track Infrared Sounder (CrIS), Visible Infrared Imaging Radiometer Suite (VIIRS) and Ozone Mapping and Profiler Suite (OMPS) instruments. The presentation purpose is to describe the JPSS project process for algorithm implementation from the very early delivering stages by the science teams to the full operationalization into the Interface Processing Segment (IDPS), the processing system that provides Environmental Data Records (EDR's) to NOAA. Special focus is given to the NASA Data Products Engineering and Services (DPES) Algorithm Integration Team (AIT) functional and regression test activities. In the functional testing phase, the AIT uses one or a few specific chunks of data (granules) selected by the NOAA STAR Calibration and Validation (cal/val) Teams to demonstrate that a small change in the code performs properly and does not disrupt the rest of the algorithm chain. In the regression testing phase, the modified code is placed into to the Government Resources for Algorithm Verification, Integration, Test and Evaluation (GRAVITE) Algorithm Development Area (ADA), a simulated and smaller version of the operational IDPS. Baseline files are swapped out, not edited and the whole code package runs in one full orbit of Science Data Records (SDR's) using Calibration Look Up Tables (Cal LUT's) for the time of the orbit. The purpose of the regression test is to

A proposition for the classification of carbonaceous chondritic micrometeorites

NASA Technical Reports Server (NTRS)

Rietmeijer, Frans J. M.

1994-01-01

Classification of interplanetary dust particles (IDP's) should be unambiguous and, if possible, provide an opportunity to interrelate these ultrafine IDP's with the matrices of undifferentiated meteorites. I prefer a scheme of chemical groupings and petrologic classes that is based on primary IDP properties that can be determined without prejudice by individual investigators. For IDP's of 2-50 microns these properties are bulk elemental chemistry, morphology, shape, and optical properties. The two major chemical groups are readily determined by energy dispersive spectroscopic analysis using the scanning or analytical electron microscope. Refinement of chondritic IDP classification is possible using the dominant mineral species, e.g. olivine, pyroxene, and layer silicates, and is readily inferred from FTIR, and automated chemical analysis. Petrographic analysis of phyllosilicate-rich IDP's will identify smectite-rich and serpentine-rich particles. Chondritic IDP's are also classified according to morphology, viz., CP and CF IDP's are aggregate particles that differ significantly in porosity, while the dense CS IDP's have a smooth surface. The CP IDP's are characterized by an anhydrous silicate mineralogy, but small amounts of layer silicates may be present. Distinction between the CP and CF IDP's is somewhat ambiguous, but the unique CP IDP's are fluffy, or porous, ultrafine-grained aggregates. The CP IDP's, which may contain silicate whiskers, are the most carbon-rich extraterrestrial material presently known. The CF IDP's are much less porous that CP IDP's. Using particle type definitions, CP IDP's in the NASA JSC Cosmic Dust Catalogs are approx. 15 percent of all IDP's that include nonchondritic spheres. Most aggregate particles are of the CF type.

Automated thin-film analyses of hydrated interplanetary dust particles in the analytical electron microscope

NASA Technical Reports Server (NTRS)

Germani, M. S.; Bradley, J. P.; Brownlee, D. E.

1990-01-01

A 200 keV electron microscope was used to obtain elemental analyses from over 4000 points on thin sections of eight 'layer silicate' class interplanetary dust particles (IDPs). Major and minor element abundances from a volume approaching that of a cylinder 50 nm in diameter were observed. Mineral phases and their relative abundances in the thin sections were identified and petrographic characteristics were determined. Three of the particles contained smectite (1.0-1.2 nm basal spacing) and two contained serpentine (0.7 nm basal spacing). The point count analyses and Mg-Si-Fe ternary diagrams show that one of the serpentine-containing IDPs is similar to CI and CM chondritic meteorites. The IDPs exhibit evidence of aqueous processing, but they have typically experienced only short range, submicrometer scale alteration. The IDPs may provide a broad sampling of the asteroid belt.

Using VIIRS to Provide Data Continuity with MODIS

NASA Technical Reports Server (NTRS)

Murphy, Robert E.; Barnes, William L.; Lyapustin, Alexei I.; Privette, Jeffrey; Welsch, Carol; DeLuccia, Frank; Schueler, Carl F.; Ardanuy, Philip E.; Kealy, Peter S. M.; Smith, David E. (Technical Monitor)

2001-01-01

Long-term continuity of the data series being initiated by the MODIS (MODerate Resolution Imaging Spectroradiometer) on NASA's Terra mission will be obtained using the VIIRS (Visible Infrared Imaging Radiometer Suite) flying on the converged National Polar-Orbiting Environmental Satellite System (NPOESS) and on the NPOESS Preparatory Project (NPP). The data series include critical parameters such as cloud and aerosol properties, vegetation index, land use and land cover, ocean chlorophyll and sea surface temperature. VIIRS is being designed and built by Raytheon for the Integrated Program Office (IPO), the DoD, NOAA and NASA consortium that is responsible for NPOESS. In addition to meeting the requirements for operational environmental monitoring, VIIRS will meet the needs of the global change research community through the use of state-of-the-art algorithms and calibration and characterization activities.

Development of Innovative Design Processor

DOE Office of Scientific and Technical Information (OSTI.GOV)

Park, Y.S.; Park, C.O.

2004-07-01

The nuclear design analysis requires time-consuming and erroneous model-input preparation, code run, output analysis and quality assurance process. To reduce human effort and improve design quality and productivity, Innovative Design Processor (IDP) is being developed. Two basic principles of IDP are the document-oriented design and the web-based design. The document-oriented design is that, if the designer writes a design document called active document and feeds it to a special program, the final document with complete analysis, table and plots is made automatically. The active documents can be written with ordinary HTML editors or created automatically on the web, which ismore » another framework of IDP. Using the proper mix-up of server side and client side programming under the LAMP (Linux/Apache/MySQL/PHP) environment, the design process on the web is modeled as a design wizard style so that even a novice designer makes the design document easily. This automation using the IDP is now being implemented for all the reload design of Korea Standard Nuclear Power Plant (KSNP) type PWRs. The introduction of this process will allow large reduction in all reload design efforts of KSNP and provide a platform for design and R and D tasks of KNFC. (authors)« less

Modeling phase separation in mixtures of intrinsically-disordered proteins

NASA Astrophysics Data System (ADS)

Gu, Chad; Zilman, Anton

Phase separation in a pure or mixed solution of intrinsically-disordered proteins (IDPs) and its role in various biological processes has generated interest from the theoretical biophysics community. Phase separation of IDPs has been implicated in the formation of membrane-less organelles such as nucleoli, as well as in a mechanism of selectivity in transport through the nuclear pore complex. Based on a lattice model of polymers, we study the phase diagram of IDPs in a mixture and describe the selective exclusion of soluble proteins from the dense-phase IDP aggregates. The model captures the essential behaviour of phase separation by a minimal set of coarse-grained parameters, corresponding to the average monomer-monomer and monomer-protein attraction strength, as well as the protein-to-monomer size ratio. Contrary to the intuition that strong monomer-monomer interaction increases exclusion of soluble proteins from the dense IDP aggregates, our model predicts that the concentration of soluble proteins in the aggregate phase as a function of monomer-monomer attraction is non-monotonic. We corroborate the predictions of the lattice model using Langevin dynamics simulations of grafted polymers in planar and cylindrical geometries, mimicking various in-vivo and in-vitro conditions.

Validation of Suomi-NPP VIIRS sea ice concentration with very high-resolution satellite and airborne camera imagery

NASA Astrophysics Data System (ADS)

Baldwin, Daniel; Tschudi, Mark; Pacifici, Fabio; Liu, Yinghui

2017-08-01

Two independent VIIRS-based Sea Ice Concentration (SIC) products are validated against SIC as estimated from Very High Spatial Resolution Imagery for several VIIRS overpasses. The 375 m resolution VIIRS SIC from the Interface Data Processing Segment (IDPS) SIC algorithm is compared against estimates made from 2 m DigitalGlobe (DG) WorldView-2 imagery and also against estimates created from 10 cm Digital Mapping System (DMS) camera imagery. The 750 m VIIRS SIC from the Enterprise SIC algorithm is compared against DG imagery. The IDPS vs. DG comparisons reveal that, due to algorithm issues, many of the IDPS SIC retrievals were falsely assigned ice-free values when the pixel was clearly over ice. These false values increased the validation bias and RMS statistics. The IDPS vs. DMS comparisons were largely over ice-covered regions and did not demonstrate the false retrieval issue. The validation results show that products from both the IDPS and Enterprise algorithms were within or very close to the 10% accuracy (bias) specifications in both the non-melting and melting conditions, but only products from the Enterprise algorithm met the 25% specifications for the uncertainty (RMS).

Carbon Raman Spectroscopy of 36 Inter-Planetary Dust Particles

NASA Technical Reports Server (NTRS)

Busemann, H.; Nittler, L. R.; Davidson, J.; Franchi, I. A.; Messenger, S.; Nakamura-Messenger, K.; Palma, R. L.; Pepin, R. O.

2009-01-01

Carbon Raman spectroscopy is a useful tool to determine the degree of order of organic material (OM) in extra-terrestrial matter. As shown for meteoritic OM [e.g., 2], peak parameters of D and G bands are a measure of thermal alteration, causing graphitization (order), and amorphization, e.g. during protoplanetary irradiation, causing disorder. Th e most pristine interplanetary dust particles (IDPs) may come from comets. However, their exact provenance is unknown. IDP collection during Earth?s passage through comet Grigg-Skjellerup?s dust stream ("GSC" collectors) may increase the probability of collecting fresh IDPs from a known, cometary source. We used Raman spectroscopy to compare 21 GSC-IDPs with 15 IDPs collected at different periods, and found that the variation among GSC-IDPs is larger than among non-GSC IDPs, with the most primitive IDPs being mostly GSC-IDPs.

The Butterflies of Principal Components: A Case of Ultrafine-Grained Polyphase Units

NASA Astrophysics Data System (ADS)

Rietmeijer, F. J. M.

1996-03-01

Dusts in the accretion regions of chondritic interplanetary dust particles [IDPs] consisted of three principal components: carbonaceous units [CUs], carbon-bearing chondritic units [GUs] and carbon-free silicate units [PUs]. Among others, differences among chondritic IDP morphologies and variable bulk C/Si ratios reflect variable mixtures of principal components. The spherical shapes of the initially amorphous principal components remain visible in many chondritic porous IDPs but fusion was documented for CUs, GUs and PUs. The PUs occur as coarse- and ultrafine-grained units that include so called GEMS. Spherical principal components preserved in an IDP as recognisable textural units have unique proporties with important implications for their petrological evolution from pre-accretion processing to protoplanet alteration and dynamic pyrometamorphism. Throughout their lifetime the units behaved as closed-systems without chemical exchange with other units. This behaviour is reflected in their mineralogies while the bulk compositions of principal components define the environments wherein they were formed.

Fibrillation mechanism of a model intrinsically disordered protein revealed by 2D correlation deep UV resonance Raman spectroscopy.

PubMed

Sikirzhytski, Vitali; Topilina, Natalya I; Takor, Gaius A; Higashiya, Seiichiro; Welch, John T; Uversky, Vladimir N; Lednev, Igor K

2012-05-14

Understanding of numerous biological functions of intrinsically disordered proteins (IDPs) is of significant interest to modern life science research. A large variety of serious debilitating diseases are associated with the malfunction of IDPs including neurodegenerative disorders and systemic amyloidosis. Here we report on the molecular mechanism of amyloid fibrillation of a model IDP (YE8) using 2D correlation deep UV resonance Raman spectroscopy. YE8 is a genetically engineered polypeptide, which is completely unordered at neutral pH yet exhibits all properties of a fibrillogenic protein at low pH. The very first step of the fibrillation process involves structural rearrangements of YE8 at the global structure level without the detectable appearance of secondary structural elements. The formation of β-sheet species follows the global structural changes and proceeds via the simultaneous formation of turns and β-strands. The kinetic mechanism revealed is an important new contribution to understanding of the general fibrillation mechanism proposed for IDP.

Infrared spectroscopy of interplanetary dust in the laboratory

NASA Technical Reports Server (NTRS)

Fraundorf, P.; Patel, R. I.; Freeman, J. J.

1981-01-01

A mount containing three crushed chondritic interplanetary dust particles (IDPs) collected in the earth's stratosphere and subjected to infrared spectroscopic measurements shows features near 1000 and 500/cm, suggesting crystalline pyroxene rather than crystalline olivine, amorphous olivine, or meteoritic clay minerals. Chondritic IDP structural diversity and atmospheric heating effects must be considered when comparing this spectrum with interplanetary and cometary dust astrophysical spectra. TEM and infrared observations of one member of the rare subset of IDPs resembling hydrated carbonaceous chondrite matrix material shows a close infrared spectrum resemblance between 4000 and 400/cm to the C2 meteorite Murchison. TEM observations suggest that this class of particles may be used as an atmospheric entry heating-process thermometer.

Longitudinal relaxation properties of 1HN and 1Hα determined by direct-detected 13C NMR experiments to study intrinsically disordered proteins (IDPs)

NASA Astrophysics Data System (ADS)

Hošek, Tomáš; Gil-Caballero, Sergi; Pierattelli, Roberta; Brutscher, Bernhard; Felli, Isabella C.

2015-05-01

Intrinsically disordered proteins (IDPs) are functional proteins containing large fragments characterized by high local mobility. Bioinformatic studies have suggested that a significant fraction (more than 30%) of eukaryotic proteins has disordered regions of more than 50 amino acids in length. Hence, NMR methods for the characterization of local compactness and solvent accessibility in such highly disordered proteins are of high importance. Among the available approaches, the HET-SOFAST/BEST experiments (Schanda et al., 2006, Rennella et al., 2014) provide semi-quantitative information by monitoring longitudinal 1H relaxation of amide protons under different initial conditions. However, when approaching physiological sample conditions, the potential of these amide 1H detected experiments is reduced due to rapid amide proton solvent exchange. 13C direct detection methods therefore provide a valuable alternative thanks to a higher chemical shift dispersion and their intrinsic insensitivity toward solvent exchange. Here we present two sets of 13C-detected experiments, which indirectly measure 1HN and 1Hα inversion recovery profiles. The experiments consist of an initial spin inversion-recovery block optimized for selective manipulation of different types of proton spins followed by a CON read-out scheme. The proposed experiments were tested on human α-synuclein and ubiquitin, two representative examples of unfolded and folded proteins.

Sequence fingerprints distinguish erroneous from correct predictions of intrinsically disordered protein regions.

PubMed

Saravanan, Konda Mani; Dunker, A Keith; Krishnaswamy, Sankaran

2017-12-27

More than 60 prediction methods for intrinsically disordered proteins (IDPs) have been developed over the years, many of which are accessible on the World Wide Web. Nearly, all of these predictors give balanced accuracies in the ~65%-~80% range. Since predictors are not perfect, further studies are required to uncover the role of amino acid residues in native IDP as compared to predicted IDP regions. In the present work, we make use of sequences of 100% predicted IDP regions, false positive disorder predictions, and experimentally determined IDP regions to distinguish the characteristics of native versus predicted IDP regions. A higher occurrence of asparagine is observed in sequences of native IDP regions but not in sequences of false positive predictions of IDP regions. The occurrences of certain combinations of amino acids at the pentapeptide level provide a distinguishing feature in the IDPs with respect to globular proteins. The distinguishing features presented in this paper provide insights into the sequence fingerprints of amino acid residues in experimentally determined as compared to predicted IDP regions. These observations and additional work along these lines should enable the development of improvements in the accuracy of disorder prediction algorithm.

Coordinates Analyses of Hydrated Interplanetary Dust Particles: Samples of Primitive Solar System Bodies

NASA Technical Reports Server (NTRS)

Keller, L. P.; Snead, C.; McKeegan, K. D.

2016-01-01

Interplanetary dust particles (IDPs) collected in the stratosphere fall into two major groups: an anhydrous group termed the "chondritic-porous (CP) IDPs and a hydrated group, the "chondritic-smooth (CS) IDPs, although rare IDPs with mineralogies intermediate between these two groups are known [1]. The CP-IDPs are widely believed to be derived from cometary sources [e.g. 2]. The hydrated CS-IDPs show mineralogical similarities to heavily aqueously altered carbonaceous chondrites (e.g. CI chondrites), but only a few have been directly linked to carbonaceous meteorite parent bodies [e.g. 3, 4]. Most CS-IDPs show distinct chemical [5] and oxygen isotopic composition differences [6-8] from primitive carbonaceous chondrites. Here, we report on our coordinated analyses of a suite of carbon-rich CS-IDPs focusing on their bulk compositions, mineralogy, mineral chemistry, and isotopic compositions.

Relations between aliphatics and silicate components in 12 stratospheric particles deduced from vibrational spectroscopy

DOE Office of Scientific and Technical Information (OSTI.GOV)

Merouane, S.; Djouadi, Z.; Le Sergeant d'Hendecourt, L., E-mail: sihane.merouane@ias.u-psud.fr

2014-01-10

Interplanetary dust particles (IDPs) are among the most pristine extraterrestrial samples available in the laboratory for analyses with moderate to high spatial- and spectral-resolution spectroscopic techniques. Their composition can provide precious information on the early stages of the solar nebula as well as on the processes on the surfaces of different small bodies in the solar system from which IDPs originate. In this work, we have analyzed six anhydrous IDPs and six stratospheric particles possibly of cosmic origin through infrared (IR) and Raman micro-spectroscopy to study and investigate their silicate and organic components. We find that the length/ramification of themore » aliphatic organics given by the CH{sub 2}/CH{sub 3} ratios in the IDPs is closely linked to the silicate family (pyroxene or olivine) present in the samples. Both IR and Raman data suggest that this relation is not correlated with either aqueous (as evidenced by the absence of aqueous related minerals) or thermal processes (as deduced from Raman measurements). Therefore, this observation might be related to the initial path of formation of the organics on the silicate surfaces, thus tracing a possible catalytic role that silicates would play in the formation and/or ramification of organic matter in the primitive nebula.« less

Stardust (Comet 81P/Wild-2) Samples and Early Solar Sys-tem Processes

NASA Astrophysics Data System (ADS)

Ebel, Denton S.; Weisberg, M. K.; Connolly, H. C.; Zolensky, M.; Mineralogy/Petrology Preliminary Examination Subteam, Stardust

2006-12-01

Dust particles from comet 81P/Wild-2 were captured in silica aerogel (also as impact debris on Al-foil strips) at 6.1 km/s relative velocity by the Stardust spacecraft on 2-Jan-2004, and returned to Earth 15-Jan-2006 [1]. A pre-liminary examination team (PET) of 150 are preparing reports on a subset of samples [2, 3, e.g., 4]. PET investigations in a short time on a limited number of <10 micron grains show that olivine, pyroxene, FeNi-metal and sulfide are common. Olivine and low-Ca pyroxene are unequilibrated in Mg/(Fe+Mg). Some for-sterite is low-iron, Mn-enriched as also found in some in-terplanetary dust particles (IDPs), and in matrix and amoe-boid olivine aggregates in CR carbonaceous chondrites (CC)[5]. Diopside and melilite are found, similar to those in spinel-pyroxene aggregates in CM chondrites and in re-fractory IDPs[6,7]. FeNi-metal and Fe-Ni, Fe-Ni-Cu and Fe-Zn sulfides are observed, and the highly reduced phase osbornite (TiN). Hydrous silicates and carbonates are not observed. A primary result is the preponderance of high temperature and reduced crystalline phases. These may form from amorphous precursors heated near the sun[8], or by viscosity-related processes farther out in the disk[9]. Silicate, metal and sulfide compositions are consistent with chondrites, particularly the CR clan. A better comparison may perhaps be made to anhydrous IDPs, which probably sample outer regions of the Solar System. The isotopic homogeneity of the grains will have important implications for mixing in the early disk. References: [1] Brownlee et al. (2004) Science 304, 1764.[2] ftp://ftp.lpi.usra.edu/pub/outgoing/lpsc2006/full101.pdf [3] Zolensky et al. (2006) LPSC XXXVII #1203. [4] Zolensky et al. (2006, in prep.) Science. [5] Weisberg et al. (2004) MaPS 39, 1741. [6] McKeegan (1987) Science 237, 1468. [7] Zolensky (1987) Science 237, 1466. [8] Scott and Krot (2005) Chondrules and the Protoplanetary Disk, 15-54. [9] Joung et al. (2004) ApJ 606, 532.

Nitrogen Isotopic Anomalies in a Hydrous Interplanetary Dust Particle

NASA Technical Reports Server (NTRS)

Smith, J. B.; Dai, Z. R.; Weber, P. K.; Graham, G. A.; Hutcheon, I. D.; Bajt, S.; Ishii, H.; Bradley, J. P.

2005-01-01

Interplanetary dust particles (IDPs) collected in the stratosphere are the fine-grained end member (5 - 50 microns in size) of the meteoritic material available for investigation in the laboratory. IDPs are derived from either cometary or asteroidal sources. Some IDPs contain cosmically primitive materials with isotopic signatures reflecting presolar origins. Recent detailed studies using the NanoSIMS have shown there is a wide variation of isotopic signatures within individual IDPs; grains with a presolar signature have been observed surrounded by material with a solar isotopic composition. The majority of IDPs studied have been anhydrous. We report here results from integrated NanoSIMS/FIB/TEM/Synchrotron IR studies of a hydrous IDP, focused on understanding the correlations between the isotopic, mineralogical and chemical compositions of IDPs.

Structured and Unstructured Binding of an Intrinsically Disordered Protein as Revealed by Atomistic Simulations.

PubMed

Ithuralde, Raúl Esteban; Roitberg, Adrián Enrique; Turjanski, Adrián Gustavo

2016-07-20

Intrinsically disordered proteins (IDPs) are a set of proteins that lack a definite secondary structure in solution. IDPs can acquire tertiary structure when bound to their partners; therefore, the recognition process must also involve protein folding. The nature of the transition state (TS), structured or unstructured, determines the binding mechanism. The characterization of the TS has become a major challenge for experimental techniques and molecular simulations approaches since diffusion, recognition, and binding is coupled to folding. In this work we present atomistic molecular dynamics (MD) simulations that sample the free energy surface of the coupled folding and binding of the transcription factor c-myb to the cotranscription factor CREB binding protein (CBP). This process has been recently studied and became a model to study IDPs. Despite the plethora of available information, we still do not know how c-myb binds to CBP. We performed a set of atomistic biased MD simulations running a total of 15.6 μs. Our results show that c-myb folds very fast upon binding to CBP with no unique pathway for binding. The process can proceed through both structured or unstructured TS's with similar probabilities. This finding reconciles previous seemingly different experimental results. We also performed Go-type coarse-grained MD of several structured and unstructured models that indicate that coupled folding and binding follows a native contact mechanism. To the best of our knowledge, this is the first atomistic MD simulation that samples the free energy surface of the coupled folding and binding processes of IDPs.

Carbon abundances, major element chemistry, and mineralogy of hydrated interplanetary dust particles

NASA Technical Reports Server (NTRS)

Keller, L. P.; Thomas, K. L.; Mckay, D. S.

1993-01-01

Hydrated interplanetary dust particles (IDP's) comprise a major fraction of the interplanetary dust particles collected in the stratosphere. While much is known about the mineralogy and chemistry of hydrated IDP's, little is known about the C abundance in this class of IDP's, the nature of the C-bearing phases, and how the C abundance is related to other physical properties of hydrated IDP's. Bulk compositional data (including C and O) for 11 hydrated IDP's that were subsequently examined by the transition electron microscopy (TEM) to determine their mineralogy and mineral chemistry are reported. Our analysis indicates that these hydrated IDP's are strongly enriched in C relative to the most C-rich meteorites. The average abundance of C in these hydrated IDP's is 4X CI chondrite values. The bulk compositions (including C and O) of 11 hydrated IDP's were determined by thin-window, energy-dispersive x ray (EDX) spectroscopy of the uncoated IDP's on Be substrates in the scanning electron microscopy (SEM). As a check on our C measurements, one of the IDP's (L2006H5) was embedded in glassy S, and microtome thin sections were prepared and placed onto Be substrates. Thin-film EDX analyses of multiple thin sections of L2006H5 show good agreement with the bulk value determined in the SEM. Following EDX analysis, the mineralogy and mineral chemistry of each IDP was determined by analyzing ultramicrotome thin sections in a TEM equipped with an EDX spectrometer.

A Small Molecule Causes a Population Shift in the Conformational Landscape of an Intrinsically Disordered Protein.

PubMed

Ban, David; Iconaru, Luigi I; Ramanathan, Arvind; Zuo, Jian; Kriwacki, Richard W

2017-10-04

Intrinsically disordered proteins (IDPs) have roles in myriad biological processes and numerous human diseases. However, kinetic and amplitude information regarding their ground-state conformational fluctuations has remained elusive. We demonstrate using nuclear magnetic resonance (NMR)-based relaxation dispersion that the D2 domain of p27 Kip1 , a prototypical IDP, samples multiple discrete, rapidly exchanging conformational states. By combining NMR with mutagenesis and small-angle X-ray scattering (SAXS), we show that these states involve aromatic residue clustering through long-range hydrophobic interactions. Theoretical studies have proposed that small molecules bind promiscuously to IDPs, causing expansion of their conformational landscapes. However, on the basis of previous NMR-based screening results, we show here that compound binding only shifts the populations of states that existed within the ground state of apo p27-D2 without changing the barriers between states. Our results provide atomic resolution insight into how a small molecule binds an IDP and emphasize the need to examine motions on the low microsecond time scale when probing these types of interactions.

The effects of interplanetary dust impacts on the accumulation of volatiles in the lunar permanently shadowed regions

NASA Astrophysics Data System (ADS)

Horanyi, Mihaly; Szalay, Jamey

2017-10-01

The lunar regolith has been formed, and remains continually reworked, by the intermitten impacts of comets, asteroids, meteoroids, and the continual bombardment by interplanetary dust particles (IDP). Thick atmospheres protect Venus, Earth, and Mars, ablating the incoming IDPs into “shooting stars” that rarely reach the surface. However, the surfaces of airless bodies near 1 AU are directly exposed to the high-speed (>> 1 km/s) IDP impacts. The Moon is expected to be bombarded by 5x103 kg/day of IDPs arriving with a characteristic speed of ~ 20 km/s. The IDP sources impacting the Moon at high latitudes remain largely uncharacterized due to the lack of optical and radar observations in the polar regions on Earth. These high latitude sources have very large impact speeds in the range of 30 < v < 50 km/ hence they are expected to have a significant effect on the lunar surface, including the removal and burial of volatile deposits in the lunar polar regions.Water is thought to be continually delivered to the Moon through geological timescales by water-bearing comets and asteroids, and produced continuously in situ by the impacts of solar wind protons of oxygen rich minerals exposed on the surface. IDPs are an unlikely source of water due to their long UV exposure in the inner solar system, but their high-speed impacts can mobilize secondary ejecta dust particles, atoms and molecules, some with high-enough speed to escape the Moon. Other surface processes that can lead to mobilization, transport and loss of water molecules and other volatiles include solar heating, photochemical processes, and solar wind sputtering. Since none of these are at work in permanently shadowed regions (PSR), dust impacts remain the dominant process to dictate the evolution of volatiles in PSRs. The competing effects of dust impacts are: a) ejecta production leading to loss out of a PSR; b) gardening and overturning the regolith; and c) the possible accumulation of impact ejecta, leading

Pre-accretional sorting of grains in the outer solar nebula

DOE Office of Scientific and Technical Information (OSTI.GOV)

Wozniakiewicz, P. J.; Bradley, J. P.; Ishii, H. A.

2013-12-20

Despite their micrometer-scale dimensions and nanogram masses, chondritic porous interplanetary dust particles (CP IDPs) are an important class of extraterrestrial material since their properties are consistent with a cometary origin and they show no evidence of significant post-accretional parent body alteration. Consequently, they can provide information about grain accretion in the comet-forming region of the outer solar nebula. We have previously reported our comparative study of the sizes and size distributions of crystalline silicate and sulfide grains in CP IDPs, in which we found these components exhibit a size-density relationship consistent with having been sorted together prior to accretion. Heremore » we extend our data set and include GEMS (glass with embedded metal and sulfide), the most abundant amorphous silicate phase observed in CP IDPs. We find that while the silicate and sulfide sorting trend previously observed is maintained, the GEMS size data do not exhibit any clear relationship to these crystalline components. Therefore, GEMS do not appear to have been sorted with the silicate and sulfide crystals. The disparate sorting trends observed in GEMS and the crystalline grains in CP IDPs present an interesting challenge for modeling early transport and accretion processes. They may indicate that several sorting mechanisms operated on these CP IDP components, or alternatively, they may simply be a reflection of different source environments.« less

The Nature and Origin of Interplanetary Dust: High Temperature Components

NASA Technical Reports Server (NTRS)

Keller, L. P.; Messenger, S.

2004-01-01

The specific parent bodies of individual interplanetary dust particles (IDPs) are un-known, but the anhydrous chondritic-porous (CP) sub-set has been linked directly to cometary sources [1]. The CP IDPs escaped the thermal processing and water-rock interactions that have severely modified or destroyed the original mineralogy of primitive meteorites. Their origin in the outer regions of the solar system suggests they should retain primitive chemical and physical characteristics from the earliest stages of solar system formation (including abundant presolar materials). Indeed, CP IDPs are the most primitive extraterrestrial materials available for laboratory studies based on their unequilibrated mineralogy [2], high concentrations of carbon, nitrogen and volatile trace elements relative to CI chondrites [3, 4, 5], presolar hydrogen and nitrogen isotopic signatures [6, 7] and abundant presolar silicates [8].

Joint Polar Satellite System

NASA Technical Reports Server (NTRS)

Trenkle, Timothy; Driggers, Phillip

2011-01-01

The Joint Polar Satellite System (JPSS) is a joint NOAA/NASA mission comprised of a series of polar orbiting weather and climate monitoring satellites which will fly in a sun-synchronous orbit, with a 1330 equatorial crossing time. JPSS resulted from the decision to reconstitute the National Polar-orbiting Operational Environmental Satellite System (NPOESS) into two separate programs, one to be run by the Department of Defense (DOD) and the other by NOAA. This decision was reached in early 2010, after numerous development issues caused a series of unacceptable delays in launching the NPOESS system.

Putting PhDs to Work: Career Planning for Today's Scientist

ERIC Educational Resources Information Center

Hobin, Jennifer A.; Clifford, Philip S.; Dunn, Ben M.; Rich, Susan; Justement, Louis B.

2014-01-01

Individual development plans (IDPs) have been promoted nationally as a tool to help research trainees explore career opportunities and set career goals. Despite the interest in IDPs from a policy perspective, there is little information about how they have been used. The authors examined IDP awareness and use, the benefits of creating an IDP, and…

Assessment of the Interstellar Processes Leading to Deuterium Enrichment in Meteoritic Organics

NASA Technical Reports Server (NTRS)

Sandford, Scott A.; Bernstein, Max P.; Dworkin, Jason P.; DeVincenzi, Donald L. (Technical Monitor)

2001-01-01

The presence of isotopic anomalies is the most unequivocal demonstration that meteoritic material contains circumstellar or interstellar components. In the case of organic compounds in meteorites and interplanetary dust particles (IDPs), the most useful isotopic tracer has been deuterium (D). We discuss four processes that are expected to lead to D enrichment in interstellar materials and describe how their unique characteristics can be used to assess their relative importance for the organics in meteorites. These enrichment processes are low temperature gas phase ion-molecule reactions, low temperature gas-grain reactions, gas phase unimolecular photodissociation, and ultraviolet photolysis in D-enriched ice mantles. Each of these processes is expected to be associated with distinct regiochemical signatures (D placement on the product molecules, correlation with specific chemical functionalities, etc.), especially in the molecular population of polycyclic aromatic hydrocarbons (PAHs). We describe these differences and discuss how they may be used to delineate the various interstellar processes that may have contributed to meteoritic D enrichments. We also briefly discuss how these processes may affect the isotopic distributions in C, 0, and N in the same compounds.

JPSS Common Ground System Multimission Support

NASA Astrophysics Data System (ADS)

Jamilkowski, M. L.; Miller, S. W.; Grant, K. D.

2013-12-01

NOAA & NASA jointly acquire the next-generation civilian operational weather satellite: Joint Polar Satellite System (JPSS). JPSS contributes the afternoon orbit & restructured NPOESS ground system (GS) to replace the current Polar-orbiting Operational Environmental Satellite (POES) system run by NOAA. JPSS sensors will collect meteorological, oceanographic, climatological & solar-geophysical observations of the earth, atmosphere & space. The JPSS GS is the Common Ground System (CGS), consisting of Command, Control, & Communications (C3S) and Interface Data Processing (IDPS) segments, both developed by Raytheon Intelligence, Information & Services (IIS). CGS now flies the Suomi National Polar-orbiting Partnership (S-NPP) satellite, transfers its mission data between ground facilities and processes its data into Environmental Data Records for NOAA & Defense (DoD) weather centers. CGS will expand to support JPSS-1 in 2017. The JPSS CGS currently does data processing (DP) for S-NPP, creating multiple TBs/day across over two dozen environmental data products (EDPs). The workload doubles after JPSS-1 launch. But CGS goes well beyond S-NPP & JPSS mission management & DP by providing data routing support to operational centers & missions worldwide. The CGS supports several other missions: It also provides raw data acquisition, routing & some DP for GCOM-W1. The CGS does data routing for numerous other missions & systems, including USN's Coriolis/Windsat, NASA's SCaN network (including EOS), NSF's McMurdo Station communications, Defense Meteorological Satellite Program (DMSP), and NOAA's POES & EUMETSAT's MetOp satellites. Each of these satellite systems orbits the Earth 14 times/day, downlinking data once or twice/orbit at up to 100s of MBs/second, to support the creation of 10s of TBs of data/day across 100s of EDPs. Raytheon and the US government invested much in Raytheon's mission-management, command & control and data-processing products & capabilities. CGS's flexible

Interplanetary Dust Particles

NASA Astrophysics Data System (ADS)

Bradley, J. P.

2003-12-01

), and isotope abundances have confirmed that the collected particles are indeed extraterrestrial and that, prior to atmospheric entry, they spent 104-105 yr as small particles orbiting the Sun (Rajan et al., 1977; Hudson et al., 1981; Bradley et al., 1984a; McKeegan et al., 1985; Messenger, 2000). (21K)Figure 1. (a)-(c) Secondary electron images. (a) Anhydrous CP IDP. (b) Hydrated CS IDP (RB12A44). (c) Single-mineral forsterite grain with adhering chondritic material. (d) Optical micrograph (transmitted light) of giant cluster IDP (U220GCA) in silicone oil on ER2 collection flag. (14K)Figure 2. Darkfield transmission electron micrographs. (a) Solar-wind sputtered rim on exterior surface plus implanted solar flare tracks in chondritic IDP U220A19 (from Bradley and Brownlee, 1986). (b) Solar flare tracks in a forsterite crystal in chondritic IDP U220B11 (from Bradley et al., 1984a). The track densities in both IDPs are ˜1010-1011 cm2 corresponding to an orbital exposure age of ˜104yr. During atmospheric entry most IDPs are frictionally heated to within 100 °C of their peak heating temperature for ˜1 s and, to a first-order approximation, the smallest particles are the least strongly heated. Although some IDPs may experience thermal pulses in excess of 1,000 °C for up to 10 s (depending on particle size, mass, entry angle, and speed) (Love and Brownlee, 1991, 1996), the presence of solar flare tracks in an IDP establishes that it was not heated above ˜650 °C ( Bradley et al., 1984a). Since IDPs decelerate from cosmic velocities at altitudes >90 km, where the maximum aerodynamic ram pressure is a factor of ˜103 less than that exerted on conventional meteorites, extremely fragile meteoritic materials that cannot survive as large objects can survive as small IDPs (Figure 1(a)) ( Brownlee, 1994). Such fragile materials are suspected to be among the most primitive objects and potentially the most informative regarding early solar system and presolar processes

Does Comet WILD-2 contain Gems?

NASA Technical Reports Server (NTRS)

Chi, M.; Ishii, H.; Dai, Z. R.; Toppani, A.; Joswiak, D. J.; Leroux, H.; Zolensky, M.; Keller, L. P.; Browning, N. D.

2007-01-01

It is expected that Comet Wild-2 dust should resemble anhydrous carbon-rich, chondritic porous (CP) interplanetary dust particles (IDPs) collected in the stratosphere because some CP IDPs are suspected to be from comets. The rarity of carbonaceous grains and presolar silicates, as well as the presence of high-temperature inner solar nebula minerals in the Wild-2 sample (e.g. osbornite and melilite), appear incompatible with most CP IDPs. However, it is premature to draw firm conclusions about the mineralogy of comet Wild-2 because only approx. 1% of the sample has been examined. The most abundant silicates in CP IDPs are GEMS (glass with embedded metal and sulfides). Nonsolar O isotopic compositions confirm that at least some GEMS in IDPs are presolar amorphous silicates. The presence or absence of GEMS in the Wild-2 sample is important because it addresses, (a) the relationship between CP IDPs and comets, and (b) the hypothesis that other GEMS in IDPs formed in the solar nebula. Here we show that most of the GEMSlike materials so far identified in Stardust aerogel were likely impact generated during collection. At the nanometer scale, they are compositionally and crystallographically distinct from GEMS in IDPs.

Porosity of an Anhydrous Chondritic Interplanetary Dust Particle

NASA Astrophysics Data System (ADS)

Strait, M. M.; Thomas, K. L.; McKay, D. S.

1995-09-01

images were cropped and reduced in size to fit the memory capabilities of the computer analysis program. The digitized images were measured using NIH Image on a Macintosh computer. Results Using a combination of tools, including density slicing, area selection and a graphics tablet, to measure porosity, the sections give values from 9 to 15% porosity. There are a variety of errors that can enter into the measurement process: (1) defining exterior surface boundaries, which can vary between sections, (2) presence of holes in the sections where grains fell out during microtoming, and (3) differentiation between the mounting medium (epoxy) and extraterrestrial components (carbonaceous material can look similar to epoxy). As noted in earlier work [6], image processing is somewhat subjective and requires careful judgment in setting the conditions for electron microscopy and digital scanning. Conclusions Typical published anhydrous IDP porosities are in the 40% range [2], a value significantly higher than the 9-15% porosity range for IDP Clu17. The porosity values for cluster particles may be more difficult to determine because both the inter- and intra-fragment porosities must be considered. The inter-fragment porosity of ~10% for Clu17 is probably much lower than the intra-fragment porosity for cluster L2008#5 because this IDP remained intact on the collection surface while the cluster particle was fragmented into hundreds of smaller units, likely reflecting an high initial cluster porosity and low coherence. We conclude that image analysis combined with microtome sectioning offers a useful new tool for porosity measurements of IDPs and other kinds of extraterrestrial materials. Acknowledgments: This work was supported by NASA/JOVE Grant #NAG8-999. References: [1] Rietmeijer F. (1993) EPSL, 117, 609. [2] Love S. et al. (1994) Icarus, 111, 227. [3] Flynn G. and Sutton S. (1991) Proc. LPS, Vol. 21, 541. [4] Thomas K. et al. (1995) GCA, in press. [5] Bradley J. (1994) Science

The Management Strategy of Benign Solitary Intraductal Papilloma on Breast Core Biopsy.

PubMed

Ko, Dayoung; Kang, Eunyoung; Park, So Yeon; Kim, Sun Mi; Jang, Mijung; Yun, Bo La; Chae, Sumin; Jang, Yerang; Kim, Hye Jin; Kim, Sung-Won; Kim, Eun-Kyu

2017-08-01

Intraductal papilloma (IDP) is well-known as one of the common benign breast lesions requiring excision. However, treatment of IDP without atypia is controversial. The aim of our study was to determine the proper management of solitary IDP by core needle biopsy (CNB). We retrospectively reviewed patients with solitary IDP confirmed by CNB from March 2003 to March 2015. We collected data about final pathology after excision, as well as clinical, histologic, and radiologic findings at initial diagnosis. The final pathology was categorized as benign or malignant. We evaluated the rate of upgrade to malignancy and factors associated with malignancy. We identified 405 patients who presented benign solitary IDP by CNB. The mean age was 46.1 years (range, 15-86 years). In total, 135 patients underwent surgical excision, and 211 underwent vacuum-assisted excision. Of 346 patients, malignant lesions were found in 8 patients (2.3%): 7 underwent surgical excision, and 1 underwent vacuum-assisted excision. Only the size of IDP was significantly associated with cancer upgrade (P = .003). Our study shows that overall malignancy upgrade rate of benign solitary IDP after excision is very low (2.3%). Even when the size of IDP was less than 1 cm, the upgrade rate to cancer was only 0.9%. Therefore, for patients with small solitary IDP, we recommend close follow-up with ultrasound instead of excision. Copyright © 2017 Elsevier Inc. All rights reserved.

Characterization of fusion genes and the significantly expressed fusion isoforms in breast cancer by hybrid sequencing

PubMed Central

Weirather, Jason L.; Afshar, Pegah Tootoonchi; Clark, Tyson A.; Tseng, Elizabeth; Powers, Linda S.; Underwood, Jason G.; Zabner, Joseph; Korlach, Jonas; Wong, Wing Hung; Au, Kin Fai

2015-01-01

We developed an innovative hybrid sequencing approach, IDP-fusion, to detect fusion genes, determine fusion sites and identify and quantify fusion isoforms. IDP-fusion is the first method to study gene fusion events by integrating Third Generation Sequencing long reads and Second Generation Sequencing short reads. We applied IDP-fusion to PacBio data and Illumina data from the MCF-7 breast cancer cells. Compared with the existing tools, IDP-fusion detects fusion genes at higher precision and a very low false positive rate. The results show that IDP-fusion will be useful for unraveling the complexity of multiple fusion splices and fusion isoforms within tumorigenesis-relevant fusion genes. PMID:26040699

Medical effects of iodine disinfection products in spacecraft water

NASA Technical Reports Server (NTRS)

Sauer, Richard L.; Janik, Daniel S.; Thorstenson, Yvonne R.

1987-01-01

Various iodination products (IDPs), including iodinated and iodine-induced new compounds, will be present in the iodine-disinfected water that is expected to be used by crews on the NASA Space Station and on long duration missions. The metabolic intermediaries created by such a process may be more important to crew health than the parent IDPs, and reclamation and recycling may be expected to produce additional products. These medical effects may be expressed in crews as hypersensitivity, allergic, acute toxic, and chronic toxic reactions, as well as modifications of immune system response.

Unfoldomics of human diseases: linking protein intrinsic disorder with diseases

PubMed Central

Uversky, Vladimir N; Oldfield, Christopher J; Midic, Uros; Xie, Hongbo; Xue, Bin; Vucetic, Slobodan; Iakoucheva, Lilia M; Obradovic, Zoran; Dunker, A Keith

2009-01-01

Background Intrinsically disordered proteins (IDPs) and intrinsically disordered regions (IDRs) lack stable tertiary and/or secondary structure yet fulfills key biological functions. The recent recognition of IDPs and IDRs is leading to an entire field aimed at their systematic structural characterization and at determination of their mechanisms of action. Bioinformatics studies showed that IDPs and IDRs are highly abundant in different proteomes and carry out mostly regulatory functions related to molecular recognition and signal transduction. These activities complement the functions of structured proteins. IDPs and IDRs were shown to participate in both one-to-many and many-to-one signaling. Alternative splicing and posttranslational modifications are frequently used to tune the IDP functionality. Several individual IDPs were shown to be associated with human diseases, such as cancer, cardiovascular disease, amyloidoses, diabetes, neurodegenerative diseases, and others. This raises questions regarding the involvement of IDPs and IDRs in various diseases. Results IDPs and IDRs were shown to be highly abundant in proteins associated with various human maladies. As the number of IDPs related to various diseases was found to be very large, the concepts of the disease-related unfoldome and unfoldomics were introduced. Novel bioinformatics tools were proposed to populate and characterize the disease-associated unfoldome. Structural characterization of the members of the disease-related unfoldome requires specialized experimental approaches. IDPs possess a number of unique structural and functional features that determine their broad involvement into the pathogenesis of various diseases. Conclusion Proteins associated with various human diseases are enriched in intrinsic disorder. These disease-associated IDPs and IDRs are real, abundant, diversified, vital, and dynamic. These proteins and regions comprise the disease-related unfoldome, which covers a significant part

Charge pattern matching as a ‘fuzzy’ mode of molecular recognition for the functional phase separations of intrinsically disordered proteins

NASA Astrophysics Data System (ADS)

Lin, Yi-Hsuan; Brady, Jacob P.; Forman-Kay, Julie D.; Chan, Hue Sun

2017-11-01

Biologically functional liquid-liquid phase separation of intrinsically disordered proteins (IDPs) is driven by interactions encoded by their amino acid sequences. Little is currently known about the molecular recognition mechanisms for distributing different IDP sequences into various cellular membraneless compartments. Pertinent physics was addressed recently by applying random-phase-approximation (RPA) polymer theory to electrostatics, which is a major energetic component governing IDP phase properties. RPA accounts for charge patterns and thus has advantages over Flory-Huggins (FH) and Overbeek-Voorn mean-field theories. To make progress toward deciphering the phase behaviors of multiple IDP sequences, the RPA formulation for one IDP species plus solvent is hereby extended to treat polyampholyte solutions containing two IDP species plus solvent. The new formulation generally allows for binary coexistence of two phases, each containing a different set of volume fractions ({φ }1,{φ }2) for the two different IDP sequences. The asymmetry between the two predicted coexisting phases with regard to their {φ }1/{φ }2 ratios for the two sequences increases with increasing mismatch between their charge patterns. This finding points to a multivalent, stochastic, ‘fuzzy’ mode of molecular recognition that helps populate various IDP sequences differentially into separate phase compartments. An intuitive illustration of this trend is provided by FH models, whereby a hypothetical case of ternary coexistence is also explored. Augmentations of the present RPA theory with a relative permittivity {ɛ }{{r}}(φ ) that depends on IDP volume fraction φ ={φ }1+{φ }2 lead to higher propensities to phase separate, in line with the case with one IDP species we studied previously. Notably, the cooperative, phase-separation-enhancing effects predicted by the prescriptions for {ɛ }{{r}}(φ ) we deem physically plausible are much more prominent than that entailed by common

The Interaction with Disabled Persons scale: revisiting its internal consistency and factor structure, and examining item-level properties.

PubMed

Iacono, Teresa; Tracy, Jane; Keating, Jenny; Brown, Ted

2009-01-01

The Interaction with Disabled Persons scale (IDP) has been used in research into baseline attitudes and to evaluate whether a shift in attitudes towards people with developmental disabilities has occurred following some form of intervention. This research has been conducted on the assumption that the IDP measures attitudes as a multidimensional construct and has good internal consistency. Such assumptions about the IDP appear flawed, particularly in light of failures to replicate its underlying factor structure. The aim of this study was to evaluate the construct validity and dimensionality of the IDP. This study used a prospective survey approach. Participants were recruited from first and second year undergraduate university students enrolled in health sciences, occupational therapy, physiotherapy, community and emergency health, nursing, and combined degrees of nursing and midwifery, and health sciences and social work at a large Australian university (n=373). Students completed the IDP, a 20-item self-report scale of attitudes towards people with disabilities. The IDP data were analysed using a combination of factor analysis (Classical Test Theory approach) and Rasch analysis (Item Response Theory approach). The results indicated that the original IDP 6-factor solution was not supported. Instead, one factor consisting of five IDP items (9, 11, 12, 17, and 18) labelled Discomfort met the four criteria for empirical validation of test quality: interval level scaling (scalability), unidimensionality, lacked of DIF across the two participant groups and data collection occasions, and hierarchical ordering. Researchers should consider using the Discomfort subscale of the IDP in future attitude research since it exhibits sound measurement properties.

Climate Suite Study for the National Polar-Orbiting Operational Environmental Satellite System Internal Concepts Study. Part A; Ozone Sensors

NASA Technical Reports Server (NTRS)

Lucke, R. L.; Planet, Walter G.; Hudson, R. D.

1995-01-01

Our recommendations to NPOESS for the sensors it should adopt to meet threshold requirements for global monitoring of ozone and, to some extent, of aerosols and of atmospheric temperature, pressure, and water vapor content are summarized in this report. The degree to which these sensors fulfill other NPOESS requirements than ozone is also summarized. The number of sensors that should be in the constellation is discussed in terms of desired reliability, continuity of coverage, and the ability to cross-calibrate successive sensors. Our recommendations for specific ozone measurement requirements, IORD item 4.1.6.2.28, are given. We make the case that the monitoring of three minor constituents in the upper atmosphere (N20, ClO or ClONO2, and HNO3) should be added to the list of NPOESS requirements because of their importance to long-term ozone studies and the small additional cost required (ozone sensors are already designed to measure them). Specific measurement requirements, which should be regarded as supplementary to the ozone requirement, are given here. The necessity of using two types of sensors, nadir-viewers and limb-scanners, for atmospheric studies is discussed.

Breast intraductal papillomas without atypia in radiologic-pathologic concordant core-needle biopsies: Rate of upgrade to carcinoma at excision.

PubMed

Pareja, Fresia; Corben, Adriana D; Brennan, Sandra B; Murray, Melissa P; Bowser, Zenica L; Jakate, Kiran; Sebastiano, Christopher; Morrow, Monica; Morris, Elizabeth A; Brogi, Edi

2016-09-15

The surgical management of mammary intraductal papilloma without atypia (IDP) identified at core-needle biopsy (CNB) is controversial. This study assessed the rate of upgrade to carcinoma at surgical excision (EXC). This study identified women with a CNB diagnosis of intraductal papilloma without atypia or carcinoma at a cancer center between 2003 and 2013. Radiologic-pathologic concordance was assessed for all cases, and discordant cases were excluded. The radiologic and clinicopathologic features of patients with a CNB diagnosis of IDP were correlated with an upgrade to carcinoma at EXC. The study population consists of 189 women with 196 IDPs; 166 women (171 IDPs) underwent EXC. The upgrade rate was 2.3% (4 of 171). The upgraded lesions were 2 invasive lobular carcinomas and 2 cases of ductal carcinoma in situ (DCIS). One case of DCIS involved the residual IDP, whereas the other 3 carcinomas were ≥ 8 mm away. Twenty-four women (25 IDPs) did not undergo EXC and had stable imaging on follow-up (median, 23.5 months). The upgrade rate at EXC for IDPs diagnosed at CNB with radiologic-pathologic concordance was 2.3%. These findings suggest that observation is appropriate for patients with radiologic-pathologic concordant CNB yielding IDP, regardless of its size. Cancer 2016. © 2016 American Cancer Society. Cancer 2016;122:2819-2827. © 2016 American Cancer Society. © 2016 American Cancer Society.

Comparison of the oxidation state of Fe in comet 81P/Wild 2 and chondritic-porous interplanetary dust particles

DOE Office of Scientific and Technical Information (OSTI.GOV)

Ogliore, Ryan C.; Butterworth, Anna L.; Fakra, Sirine C.

2010-07-16

The fragile structure of chondritic-porous interplanetary dust particles (CP-IDPs) and their minimal parent-body alteration have led researchers to believe these particles originate in comets rather than asteroids where aqueous and thermal alterations have occurred. The solar elemental abundances and atmospheric entry speed of CP-IDPs also suggest a cometary origin. With the return of the Stardust samples from Jupiter-family comet 81P/Wild 2, this hypothesis can be tested. We have measured the Fe oxidation state of 15 CP-IDPs and 194 Stardust fragments using a synchrotron-based x-ray microprobe. We analyzed {approx}300 ng of Wild 2 material - three orders of magnitude more materialmore » than other analyses comparing Wild 2 and CP-IDPs. The Fe oxidation state of these two samples of material are > 2{sigma} different: the CP-IDPs are more oxidized than the Wild 2 grains. We conclude that comet Wild 2 contains material that formed at a lower oxygen fugacity than the parent-body, or parent bodies, of CP-IDPs. If all Jupiter-family comets are similar, they do not appear to be consistent with the origin of CP-IDPs. However, comets that formed from a different mix of nebular material and are more oxidized than Wild 2 could be the source of CP-IDPs.« less

Water and organics in interplanetary dust particles

NASA Astrophysics Data System (ADS)

Bradley, John

Interplanetary dust particles (IDPs) and larger micrometeorites (MMs) impinge on the upper atmosphere where they decelerate at 90 km altitude and settle to the Earths surface. Comets and asteroids are the major sources and the flux, 30,000-40,000 tons/yr, is comparable to the mass of larger meteorites impacting the Earths surface. The sedimentary record suggests that the flux was much higher on the early Earth. The chondritic porous (CP) subset of IDPs together with their larger counterparts, ultracarbonaceous micrometeorites (UCMMs), appear to be unique among known meteoritic materials in that they are composed almost exclusively of anhydrous minerals, some of them contain >> 50% organic carbon by volume as well as the highest abundances of presolar silicate grains including GEMS. D/H and 15N abundances implicate the Oort Cloud or presolar molecular cloud as likely sources of the organic carbon. Prior to atmospheric entry, IDPs and MMs spend 104-105 year lifetimes in solar orbit where their surfaces develop amorphous space weathered rims from exposure to the solar wind (SW). Similar rims are observed on lunar soil grains and on asteroid Itokawa regolith grains. Using valence electron energy-loss spectroscopy (VEELS) we have detected radiolytic water in the rims on IDPs formed by the interaction of solar wind protons with oxygen in silicate minerals. Therefore, IDPs and MMs continuously deliver both water and organics to the earth and other terrestrial planets. The interaction of protons with oxygen-rich minerals to form water is a universal process.

Genome-wide analysis of protein disorder in Arabidopsis thaliana: implications for plant environmental adaptation.

PubMed

Pietrosemoli, Natalia; García-Martín, Juan A; Solano, Roberto; Pazos, Florencio

2013-01-01

Intrinsically disordered proteins/regions (IDPs/IDRs) are currently recognized as a widespread phenomenon having key cellular functions. Still, many aspects of the function of these proteins need to be unveiled. IDPs conformational flexibility allows them to recognize and interact with multiple partners, and confers them larger interaction surfaces that may increase interaction speed. For this reason, molecular interactions mediated by IDPs/IDRs are particularly abundant in certain types of protein interactions, such as those of signaling and cell cycle control. We present the first large-scale study of IDPs in Arabidopsis thaliana, the most widely used model organism in plant biology, in order to get insight into the biological roles of these proteins in plants. The work includes a comparative analysis with the human proteome to highlight the differential use of disorder in both species. Results show that while human proteins are in general more disordered, certain functional classes, mainly related to environmental response, are significantly more enriched in disorder in Arabidopsis. We propose that because plants cannot escape from environmental conditions as animals do, they use disorder as a simple and fast mechanism, independent of transcriptional control, for introducing versatility in the interaction networks underlying these biological processes so that they can quickly adapt and respond to challenging environmental conditions.

Multifarious Roles of Intrinsic Disorder in Proteins Illustrate Its Broad Impact on Plant Biology

PubMed Central

Sun, Xiaolin; Rikkerink, Erik H.A.; Jones, William T.; Uversky, Vladimir N.

2013-01-01

Intrinsically disordered proteins (IDPs) are highly abundant in eukaryotic proteomes. Plant IDPs play critical roles in plant biology and often act as integrators of signals from multiple plant regulatory and environmental inputs. Binding promiscuity and plasticity allow IDPs to interact with multiple partners in protein interaction networks and provide important functional advantages in molecular recognition through transient protein–protein interactions. Short interaction-prone segments within IDPs, termed molecular recognition features, represent potential binding sites that can undergo disorder-to-order transition upon binding to their partners. In this review, we summarize the evidence for the importance of IDPs in plant biology and evaluate the functions associated with intrinsic disorder in five different types of plant protein families experimentally confirmed as IDPs. Functional studies of these proteins illustrate the broad impact of disorder on many areas of plant biology, including abiotic stress, transcriptional regulation, light perception, and development. Based on the roles of disorder in the protein–protein interactions, we propose various modes of action for plant IDPs that may provide insight for future experimental approaches aimed at understanding the molecular basis of protein function within important plant pathways. PMID:23362206

Free-energy landscape of intrinsically disordered proteins investigated by all-atom multicanonical molecular dynamics.

PubMed

Higo, Junichi; Umezawa, Koji

2014-01-01

We introduce computational studies on intrinsically disordered proteins (IDPs). Especially, we present our multicanonical molecular dynamics (McMD) simulations of two IDP-partner systems: NRSF-mSin3 and pKID-KIX. McMD is one of enhanced conformational sampling methods useful for conformational sampling of biomolecular systems. IDP adopts a specific tertiary structure upon binding to its partner molecule, although it is unstructured in the unbound state (i.e. the free state). This IDP-specific property is called "coupled folding and binding". The McMD simulation treats the biomolecules with an all-atom model immersed in an explicit solvent. In the initial configuration of simulation, IDP and its partner molecules are set to be distant from each other, and the IDP conformation is disordered. The computationally obtained free-energy landscape for coupled folding and binding has shown that native- and non-native-complex clusters distribute complicatedly in the conformational space. The all-atom simulation suggests that both of induced-folding and population-selection are coupled complicatedly in the coupled folding and binding. Further analyses have exemplified that the conformational fluctuations (dynamical flexibility) in the bound and unbound states are essentially important to characterize IDP functioning.

Programming molecular self-assembly of intrinsically disordered proteins containing sequences of low complexity

NASA Astrophysics Data System (ADS)

Simon, Joseph R.; Carroll, Nick J.; Rubinstein, Michael; Chilkoti, Ashutosh; López, Gabriel P.

2017-06-01

Dynamic protein-rich intracellular structures that contain phase-separated intrinsically disordered proteins (IDPs) composed of sequences of low complexity (SLC) have been shown to serve a variety of important cellular functions, which include signalling, compartmentalization and stabilization. However, our understanding of these structures and our ability to synthesize models of them have been limited. We present design rules for IDPs possessing SLCs that phase separate into diverse assemblies within droplet microenvironments. Using theoretical analyses, we interpret the phase behaviour of archetypal IDP sequences and demonstrate the rational design of a vast library of multicomponent protein-rich structures that ranges from uniform nano-, meso- and microscale puncta (distinct protein droplets) to multilayered orthogonally phase-separated granular structures. The ability to predict and program IDP-rich assemblies in this fashion offers new insights into (1) genetic-to-molecular-to-macroscale relationships that encode hierarchical IDP assemblies, (2) design rules of such assemblies in cell biology and (3) molecular-level engineering of self-assembled recombinant IDP-rich materials.

Infrared Spectroscopy of Anhydrous Interplanetary Dust Particles

NASA Technical Reports Server (NTRS)

Keller, L. P.; Flynn, G. J.

2003-01-01

Infrared (IR) spectroscopy is the primary means of mineralogical analysis of materials outside our solar system. The identity and properties of circumstellar grains are inferred from spectral comparisons between astronomical observations and laboratory data from natural and synthetic materials. These comparisons have been facilitated by the Infrared Space Observatory (ISO), which obtained IR spectra from numerous astrophysical objects over a wide spectral range (out to 50/cm) where crystalline silicates and other phases have distinct features. The anhydrous interplanetary dust particles (IDPs) are particularly important comparison materials because some IDPs contain carbonaceous material with non-solar D/H and N-15/N-14 ratios and amorphous and crystalline silicates with non-solar 0- isotopic ratios, demonstrating that these IDPs contain preserved interstellar material. Here, we report on micro- Fourier transform (FT) IR spectrometry of IDPs, focusing on the inorganic components of primitive IDPs (FTIR spectra from the organic/carbonacecous materials in IDPs are described elsewhere).

A J-modulated protonless NMR experiment characterizes the conformational ensemble of the intrinsically disordered protein WIP.

PubMed

Rozentur-Shkop, Eva; Goobes, Gil; Chill, Jordan H

2016-12-01

Intrinsically disordered proteins (IDPs) are multi-conformational polypeptides that lack a single stable three-dimensional structure. It has become increasingly clear that the versatile IDPs play key roles in a multitude of biological processes, and, given their flexible nature, NMR is a leading method to investigate IDP behavior on the molecular level. Here we present an IDP-tailored J-modulated experiment designed to monitor changes in the conformational ensemble characteristic of IDPs by accurately measuring backbone one- and two-bond J( 15 N, 13 Cα) couplings. This concept was realized using a unidirectional (H)NCO 13 C-detected experiment suitable for poor spectral dispersion and optimized for maximum coverage of amino acid types. To demonstrate the utility of this approach we applied it to the disordered actin-binding N-terminal domain of WASp interacting protein (WIP), a ubiquitous key modulator of cytoskeletal changes in a range of biological systems. One- and two-bond J( 15 N, 13 Cα) couplings were acquired for WIP residues 2-65 at various temperatures, and in denaturing and crowding environments. Under native conditions fitted J-couplings identified in the WIP conformational ensemble a propensity for extended conformation at residues 16-23 and 45-60, and a helical tendency at residues 28-42. These findings are consistent with a previous study of the based upon chemical shift and RDC data and confirm that the WIP 2-65 conformational ensemble is biased towards the structure assumed by this fragment in its actin-bound form. The effects of environmental changes upon this ensemble were readily apparent in the J-coupling data, which reflected a significant decrease in structural propensity at higher temperatures, in the presence of 8 M urea, and under the influence of a bacterial cell lysate. The latter suggests that crowding can cause protein unfolding through protein-protein interactions that stabilize the unfolded state. We conclude that J-couplings are a

A road damage and life-cycle greenhouse gas comparison of trucking and pipeline water delivery systems for hydraulically fractured oil and gas field development in Colorado

PubMed Central

Duthu, Ray C.

2017-01-01

The process of hydraulic fracturing for recovery of oil and natural gas uses large amounts of fresh water and produces a comparable amount of wastewater, much of which is typically transported by truck. Truck transport of water is an expensive and energy-intensive process with significant external costs including roads damages, and pollution. The integrated development plan (IDP) is the industry nomenclature for an integrated oil and gas infrastructure system incorporating pipeline-based transport of water and wastewater, centralized water treatment, and high rates of wastewater recycling. These IDP have been proposed as an alternative to truck transport systems so as to mitigate many of the economic and environmental problems associated with natural gas production, but the economic and environmental performance of these systems have not been analyzed to date. This study presents a quantification of lifecycle greenhouse gas (GHG) emissions and road damages of a generic oil and gas field, and of an oil and gas development sited in the Denver-Julesburg basin in the northern Colorado region of the US. Results demonstrate that a reduction in economic and environmental externalities can be derived from the development of these IDP-based pipeline water transportation systems. IDPs have marginal utility in reducing GHG emissions and road damage when they are used to replace in-field water transport, but can reduce GHG emissions and road damage by factors of as much as 6 and 7 respectively, when used to replace fresh water transport and waste-disposal routes for exemplar Northern Colorado oil and gas fields. PMID:28686682

A road damage and life-cycle greenhouse gas comparison of trucking and pipeline water delivery systems for hydraulically fractured oil and gas field development in Colorado.

PubMed

Duthu, Ray C; Bradley, Thomas H

2017-01-01

The process of hydraulic fracturing for recovery of oil and natural gas uses large amounts of fresh water and produces a comparable amount of wastewater, much of which is typically transported by truck. Truck transport of water is an expensive and energy-intensive process with significant external costs including roads damages, and pollution. The integrated development plan (IDP) is the industry nomenclature for an integrated oil and gas infrastructure system incorporating pipeline-based transport of water and wastewater, centralized water treatment, and high rates of wastewater recycling. These IDP have been proposed as an alternative to truck transport systems so as to mitigate many of the economic and environmental problems associated with natural gas production, but the economic and environmental performance of these systems have not been analyzed to date. This study presents a quantification of lifecycle greenhouse gas (GHG) emissions and road damages of a generic oil and gas field, and of an oil and gas development sited in the Denver-Julesburg basin in the northern Colorado region of the US. Results demonstrate that a reduction in economic and environmental externalities can be derived from the development of these IDP-based pipeline water transportation systems. IDPs have marginal utility in reducing GHG emissions and road damage when they are used to replace in-field water transport, but can reduce GHG emissions and road damage by factors of as much as 6 and 7 respectively, when used to replace fresh water transport and waste-disposal routes for exemplar Northern Colorado oil and gas fields.

Characterization of fusion genes and the significantly expressed fusion isoforms in breast cancer by hybrid sequencing.

PubMed

Weirather, Jason L; Afshar, Pegah Tootoonchi; Clark, Tyson A; Tseng, Elizabeth; Powers, Linda S; Underwood, Jason G; Zabner, Joseph; Korlach, Jonas; Wong, Wing Hung; Au, Kin Fai

2015-10-15

We developed an innovative hybrid sequencing approach, IDP-fusion, to detect fusion genes, determine fusion sites and identify and quantify fusion isoforms. IDP-fusion is the first method to study gene fusion events by integrating Third Generation Sequencing long reads and Second Generation Sequencing short reads. We applied IDP-fusion to PacBio data and Illumina data from the MCF-7 breast cancer cells. Compared with the existing tools, IDP-fusion detects fusion genes at higher precision and a very low false positive rate. The results show that IDP-fusion will be useful for unraveling the complexity of multiple fusion splices and fusion isoforms within tumorigenesis-relevant fusion genes. © The Author(s) 2015. Published by Oxford University Press on behalf of Nucleic Acids Research.

Impact of interventions on malaria in internally displaced persons along the China-Myanmar border: 2011-2014.

PubMed

Zhou, Guofa; Lo, Eugenia; Zhong, Daibin; Wang, Xiaoming; Wang, Ying; Malla, Sameer; Lee, Ming-Chieh; Yang, Zhaoqing; Cui, Liwang; Yan, Guiyun

2016-09-15

Internally displaced persons (IDP) represent vulnerable populations whose public health conditions merit special attention. In the China-Myanmar border area, human movement and resettlements of IDP can influence malaria transmission. Comparison of disease incidence and vector densities between IDP camps and surrounding local villages allows for better understanding of current epidemiology and to evaluate the effectiveness of interventions in the region. Malaria and vector surveillance was conducted in three IDP camps and three local villages neighbouring the camps along the China-Myanmar border in Myanmar. Clinical malaria cases were collected from seven hospitals/clinics from April 2011 to December 2014. Malaria vector population dynamics were monitored using CDC light traps. The use of malaria preventive measures and information on aid agencies and their activities was obtained through questionnaire surveys. Malaria was confirmed in 1832 patients. Of these cases, 85.4 % were Plasmodium vivax and 11.4 % were Plasmodium falciparum malaria. Annual malaria incidence rates were 38.8 and 127.0 cases/1000 person year in IDP camps and local villages, respectively. Older children of 5-14 years had the highest incidence rate in the camps regardless of gender, while male adults had significantly higher incidence rates than females in local villages and females child-bearing age had significantly lower risk to malaria in IDP camps compare to local villages. Seasonal malaria outbreaks were observed both in the IDP camps and in the local villages from May to August 2013. The proportion of P. vivax remained unchanged in local villages but increased by approximately tenfold in IDP camps from 2011 to 2014. Anopheles vector density was tenfold higher in local villages compared to IDP camps (2.0:0.2 females/trap/night). Over 99 % of households in both communities owned bed nets. While long-lasting insecticidal nets accounted for 61 % of nets used in IDPs, nearly all residents

Deciphering the Dynamic Interaction Profile of an Intrinsically Disordered Protein by NMR Exchange Spectroscopy.

PubMed

Delaforge, Elise; Kragelj, Jaka; Tengo, Laura; Palencia, Andrés; Milles, Sigrid; Bouvignies, Guillaume; Salvi, Nicola; Blackledge, Martin; Jensen, Malene Ringkjøbing

2018-01-24

Intrinsically disordered proteins (IDPs) display a large number of interaction modes including folding-upon-binding, binding without major structural transitions, or binding through highly dynamic, so-called fuzzy, complexes. The vast majority of experimental information about IDP binding modes have been inferred from crystal structures of proteins in complex with short peptides of IDPs. However, crystal structures provide a mainly static view of the complexes and do not give information about the conformational dynamics experienced by the IDP in the bound state. Knowledge of the dynamics of IDP complexes is of fundamental importance to understand how IDPs engage in highly specific interactions without concomitantly high binding affinity. Here, we combine rotating-frame R 1ρ , Carr-Purcell-Meiboom Gill relaxation dispersion as well as chemical exchange saturation transfer to decipher the dynamic interaction profile of an IDP in complex with its partner. We apply the approach to the dynamic signaling complex formed between the mitogen-activated protein kinase (MAPK) p38α and the intrinsically disordered regulatory domain of the MAPK kinase MKK4. Our study demonstrates that MKK4 employs a subtle combination of interaction modes in order to bind to p38α, leading to a complex displaying significantly different dynamics across the bound regions.

Modeling disordered protein interactions from biophysical principles

PubMed Central

Christoffer, Charles; Terashi, Genki

2017-01-01

Disordered protein-protein interactions (PPIs), those involving a folded protein and an intrinsically disordered protein (IDP), are prevalent in the cell, including important signaling and regulatory pathways. IDPs do not adopt a single dominant structure in isolation but often become ordered upon binding. To aid understanding of the molecular mechanisms of disordered PPIs, it is crucial to obtain the tertiary structure of the PPIs. However, experimental methods have difficulty in solving disordered PPIs and existing protein-protein and protein-peptide docking methods are not able to model them. Here we present a novel computational method, IDP-LZerD, which models the conformation of a disordered PPI by considering the biophysical binding mechanism of an IDP to a structured protein, whereby a local segment of the IDP initiates the interaction and subsequently the remaining IDP regions explore and coalesce around the initial binding site. On a dataset of 22 disordered PPIs with IDPs up to 69 amino acids, successful predictions were made for 21 bound and 18 unbound receptors. The successful modeling provides additional support for biophysical principles. Moreover, the new technique significantly expands the capability of protein structure modeling and provides crucial insights into the molecular mechanisms of disordered PPIs. PMID:28394890

Structural biology of intrinsically disordered proteins: Revisiting unsolved mysteries.

PubMed

Sigalov, Alexander B

2016-06-01

The emergence of intrinsically disordered proteins (IDPs) has challenged the classical protein structure-function paradigm by introducing a new paradigm of "coupled binding and folding". This paradigm suggests that IDPs fold upon binding to their partners. Further studies, however, revealed a novel and previously unrecognized phenomenon of "uncoupled binding and folding" suggesting that IDPs do not necessarily fold upon interaction with their lipid and protein partners. The complex and often unusual biophysics of IDPs makes structural characterization of these proteins and their complexes not only challenging but often resulting in opposite conclusions. For this reason, some crucial questions in this field remain unsolved for well over a decade. Considering an important role of IDPs in cellular regulation, signaling and control in health and disease, more efforts are needed to solve these mysteries. Here, I focus on two long-standing contradictions in the literature concerning dimerization and membrane-binding activities of IDPs. Molecular explanation of these discrepancies is provided. I also demonstrate how resolution of these critical issues in the field of IDPs results in our expanded understanding of cell function and has multiple applications in biology and medicine. Copyright © 2016 Elsevier B.V. and Société Française de Biochimie et Biologie Moléculaire (SFBBM). All rights reserved.

Identification of a Compound Spinel and Silicate Presolar Grain in a Chondritic Interplanetary Dust Particle

NASA Technical Reports Server (NTRS)

Nguyen, A. N.; Nakamura-Messenger, K.; Messenger, S.; Keller, L. P.; Kloeck, W.

2014-01-01

Anhydrous chondritic porous interplanetary dust particles (CP IDPs) have undergone minimal parent body alteration and contain an assemblage of highly primitive materials, including molecular cloud material, presolar grains, and material that formed in the early solar nebula [1-3]. The exact parent bodies of individual IDPs are not known, but IDPs that have extremely high abundances of presolar silicates (up to 1.5%) most likely have cometary origins [1, 4]. The presolar grain abundance among these minimally altered CP IDPs varies widely. "Isotopically primitive" IDPs distinguished by anomalous bulk N isotopic compositions, numerous 15N-rich hotspots, and some C isotopic anomalies have higher average abundances of presolar grains (375 ppm) than IDPs with isotopically normal bulk N (<10 ppm) [5]. Some D and N isotopic anomalies have been linked to carbonaceous matter, though this material is only rarely isotopically anomalous in C [1, 5, 6]. Previous studies of the bulk chemistry and, in some samples, the mineralogy of select anhydrous CP IDPs indicate a link between high C abundance and pyroxene-dominated mineralogy [7]. In this study, we conduct coordinated mineralogical and isotopic analyses of samples that were analyzed by [7] to characterize isotopically anomalous materials and to establish possible correlations with C abundance.

An introduction to the interim digital SAR processor and the characteristics of the associated Seasat SAR imagery

NASA Technical Reports Server (NTRS)

Wu, C.; Barkan, B.; Huneycutt, B.; Leang, C.; Pang, S.

1981-01-01

Basic engineering data regarding the Interim Digital SAR Processor (IDP) and the digitally correlated Seasat synthetic aperature radar (SAR) imagery are presented. The correlation function and IDP hardware/software configuration are described, and a preliminary performance assessment presented. The geometric and radiometric characteristics, with special emphasis on those peculiar to the IDP produced imagery, are described.

Refugee Education: The State of Nigeria's Preparedness

ERIC Educational Resources Information Center

Obashoro-John, Oluwayemisi A.; Oni, Gbolabo J.

2017-01-01

The spate of insurgences and conflicts in the country and around the sub-region has led to the increased presence of refugees and Internally Displaced People (IDPs) in Nigeria. This has resultant challenges on the basic needs of refugees and IDPs at different levels. One of the highest priorities of refugees and IDPs communities is education.…

Mortality and health among internally displaced persons in western Kenya following post-election violence, 2008: novel use of demographic surveillance.

PubMed

Feikin, Daniel R; Adazu, Kubaje; Obor, David; Ogwang, Sheila; Vulule, John; Hamel, Mary J; Laserson, Kayla

2010-08-01

To evaluate mortality and morbidity among internally displaced persons (IDPs) who relocated in a demographic surveillance system (DSS) area in western Kenya following post-election violence. In 2007, 204 000 individuals lived in the DSS area, where field workers visit households every 4 months to record migrations, births and deaths. We collected data on admissions among children < 5 years of age in the district hospital and developed special questionnaires to record information on IDPs. Mortality, migration and hospitalization rates among IDPs and regular DSS residents were compared, and verbal autopsies were performed for deaths. Between December 2007 and May 2008, 16 428 IDPs migrated into the DSS, and over half of them stayed 6 months or longer. In 2008, IDPs aged 15-49 years died at higher rates than regular residents of the DSS (relative risk, RR: 1.34; 95% confidence interval, CI: 1.004-1.80). A greater percentage of deaths from human immunodeficiency virus (HIV) infection occurred among IDPs aged > or = 5 years (53%) than among regular DSS residents (25-29%) (P < 0.001). Internally displaced children < 5 years of age did not die at higher rates than resident children but were hospitalized at higher rates (RR: 2.95; 95% CI: 2.44-3.58). HIV-infected internally displaced adults in conflict-ridden parts of Africa are at increased risk of HIV-related death. Relief efforts should extend to IDPs who have relocated outside IDP camps, particularly if afflicted with HIV infection or other chronic conditions.

Electrostatically Accelerated Coupled Binding and Folding of Intrinsically Disordered Proteins

PubMed Central

Ganguly, Debabani; Otieno, Steve; Waddell, Brett; Iconaru, Luigi; Kriwacki, Richard W.; Chen, Jianhan

2012-01-01

Intrinsically disordered proteins (IDPs) are now recognized to be prevalent in biology, and many potential functional benefits have been discussed. However, the frequent requirement of peptide folding in specific interactions of IDPs could impose a kinetic bottleneck, which could be overcome only by efficient folding upon encounter. Intriguingly, existing kinetic data suggest that specific binding of IDPs is generally no slower than that of globular proteins. Here, we exploited the cell cycle regulator p27Kip1 (p27) as a model system to understand how IDPs might achieve efficient folding upon encounter for facile recognition. Combining experiments and coarse-grained modeling, we demonstrate that long-range electrostatic interactions between enriched charges on p27 and near its binding site on cyclin A not only enhance the encounter rate (i.e., electrostatic steering), but also promote folding-competent topologies in the encounter complexes, allowing rapid subsequent formation of short-range native interactions en route to the specific complex. In contrast, nonspecific hydrophobic interactions, while hardly affecting the encounter rate, can significantly reduce the efficiency of folding upon encounter and lead to slower binding kinetics. Further analysis of charge distributions in a set of known IDP complexes reveals that, although IDP binding sites tend to be more hydrophobic compared to the rest of the target surface, their vicinities are frequently enriched with charges to complement those on IDPs. This observation suggests that electrostatically accelerated encounter and induced folding might represent a prevalent mechanism for promoting facile IDP recognition. PMID:22721951

Utilization of protein intrinsic disorder knowledge in structural proteomics

PubMed Central

Oldfield, Christopher J.; Xue, Bin; Van, Ya-Yue; Ulrich, Eldon L.; Markley, John L.; Dunker, A. Keith; Uversky, Vladimir N.

2014-01-01

Intrinsically disordered proteins (IDPs) and proteins with long disordered regions are highly abundant in various proteomes. Despite their lack of well-defined ordered structure, these proteins and regions are frequently involved in crucial biological processes. Although in recent years these proteins have attracted the attention of many researchers, IDPs represent a significant challenge for structural characterization since these proteins can impact many of the processes in the structure determination pipeline. Here we investigate the effects of IDPs on the structure determination process and the utility of disorder prediction in selecting and improving proteins for structural characterization. Examination of the extent of intrinsic disorder in existing crystal structures found that relatively few protein crystal structures contain extensive regions of intrinsic disorder. Although intrinsic disorder is not the only cause of crystallization failures and many structured proteins cannot be crystallized, filtering out highly disordered proteins from structure-determination target lists is still likely to be cost effective. Therefore it is desirable to avoid highly disordered proteins from structure-determination target lists and we show that disorder prediction can be applied effectively to enrich structure determination pipelines with proteins more likely to yield crystal structures. For structural investigation of specific proteins, disorder prediction can be used to improve targets for structure determination. Finally, a framework for considering intrinsic disorder in the structure determination pipeline is proposed. PMID:23232152

Ocean observer study: A proposed national asset to augment the future U.S. operational satellite system

USGS Publications Warehouse

Cunningham, J.D.; Chambers, D.; Davis, C.O.; Gerber, A.; Helz, R.; McGuire, J.P.; Pichel, W.

2003-01-01

The next generation of U.S. polar orbiting environmental satellites, are now under development. These satellites, jointly developed by the Department of Defense (DoD), the Department of Commerce (DOC), and the National Aeronautics and Space Administration (NASA), will be known as the National Polar-orbiting Operational Environmental Satellite System (NPOESS). It is expected that the first of these satellites will be launched in 2010. NPOESS has been designed to meet the operational needs of the U.S. civilian meteorological, environmental, climatic, and space environmental remote sensing programs, and the Global Military Space and Geophysical Environmental remote sewing programs. This system, however, did not meet all the needs of the user community interested in operational oceanography (particularly in coastal regions). Beginning in the fall of 2000, the Integrated Program Office (IPO), a joint DoD, DOC, and NASA office responsible for the NPOESS development, initiated the Ocean Observer Study (OOS). The purpose of this study was to assess and recommend how best to measure the missing or inadequately sampled ocean parameters. This paper summarizes the ocean measurement requirements documented in the OOS, describes the national need to measure these parameters, and describes the satellite instrumentation required to make those measurements.

Combined infrared and analytical electron microscope studies of interplanetary dust particles

NASA Technical Reports Server (NTRS)

Bradley, J. P.; Humecki, H. J.; Germani, M. S.

1992-01-01

Ultramicrotomed thin sections (less than 100 nm thick) of eight chondritic interplanetary dust particles (IDPs) were studied by analytical electron microscopy and IR microspectroscopy with the objective of identifying IDPs or their specific components with IR spectral transmission characteristics at 10 microns similar to those of comets. Two IDPs are identified whose silicate emission characteristics between 8 and 12 microns are similar to those of comets Halley and Bradfield. Implanted solar flare tracks and sputtered rims resulting from solar wind damage suggest that the minerology and petrography of these IDPs have not been significantly perturbed since ejection from their parent bodies.

Mapping multivalency and differential affinities within large intrinsically disordered protein complexes with segmental motion analysis.

PubMed

Milles, Sigrid; Lemke, Edward A

2014-07-07

Intrinsically disordered proteins (IDPs) can bind to multiple interaction partners. Numerous binding regions in the IDP that act in concert through complex cooperative effects facilitate such interactions, but complicate studying IDP complexes. To address this challenge we developed a combined fluorescence correlation and time-resolved polarization spectroscopy approach to study the binding properties of the IDP nucleoporin153 (Nup153) to nuclear transport receptors (NTRs). The detection of segmental backbone mobility of Nup153 within the unperturbed complex provided a readout of local, region-specific binding properties that are usually masked in measurements of the whole IDP. The binding affinities of functionally and structurally diverse NTRs to distinct regions of Nup153 can differ by orders of magnitudes-a result with implications for the diversity of transport routes in nucleocytoplasmic transport. © 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

An Interesting Case of Oral Inverted Ductal Papilloma.

PubMed

Berridge, Natasha; Kumar, Mahesh

2016-12-01

Ductal papillomas are rare benign papillary tumours of the minor salivary glands. Previously they have been classified into three subtypes: inverted ductal papilloma (IDP), sialadenoma papilliferum and intraductal papilloma. The oral inverted ductal papilloma is the least common of these lesions, with 48 cases reported to date in a search of the English-language literature. We describe an interesting case of oral IDP and highlight the pertinent clinical and histopathological features. Importantly, unlike the histologically similar inverted papilloma (IP) of the nose and paranasal sinuses, oral IDP is a completely benign lesion and is not associated with malignant change. Clinical relevance: This case report aims to raise awareness of the lesion, oral inverted ductal papilloma (IDP). We outline the clinical features and unique histopathology of oral IDP so that clinicians may correctly diagnose such a lesion and implement appropriate treatment.

Disorder-function relationships for the cell cycle regulatory proteins p21 and p27.

PubMed

Mitrea, Diana M; Yoon, Mi-Kyung; Ou, Li; Kriwacki, Richard W

2012-04-01

The classic structure-function paradigm has been challenged by a recently identified class of proteins: intrinsically disordered proteins (IDPs). Despite their lack of stable secondary or tertiary structure, IDPs are prevalent in all forms of life and perform myriad cellular functions, including signaling and regulation. Importantly, disruption of IDP homeostasis is associated with numerous human diseases, including cancer and neurodegeneration. Despite wide recognition of IDPs, the molecular mechanisms underlying their functions are not fully understood. Here we review the structural features and disorder-function relationships for p21 and p27, two cyclin-dependent kinase (Cdk) regulators involved in controlling cell division and fate. Studies of p21 bound to Cdk2/cyclin A revealed that a helix stretching mechanism mediates binding promiscuity. Further, investigations of Tyr88-phosphorylated p27 identified a signaling conduit that controls cell division and is disrupted in certain cancers. These mechanisms rely upon a balance between nascent structure in the free state, induced folding upon binding, and persistent flexibility within functional complexes. Although these disorder-function relationships are likely to be recapitulated in other IDPs, it is also likely that the vocabulary of their mechanisms is much more extensive than is currently understood. Further study of the physical properties of IDPs and elucidation of their links with function are needed to fully understand the mechanistic language of IDPs.

Ethical challenges in mental health research among internally displaced people: ethical theory and research implementation

PubMed Central

2013-01-01

Millions of people undergo displacement in the world. Internally displaced people (IDP) are especially vulnerable as they are not protected by special legislation in contrast to other migrants. Research conducted among IDPs must be correspondingly sensitive in dealing with ethical issues that may arise. Muslim IDPs in Puttalam district in the North-Western province of Sri Lanka were initially displaced from Northern Sri Lanka due to the conflict in 1991. In the backdrop of a study exploring the prevalence of common mental disorders among the IDPs, researchers encountered various ethical challenges. These included inter-related issues of autonomy, non-maleficence, beneficence, confidentiality and informed consent, and how these were tailored in a culture-specific way to a population that has increased vulnerability. This paper analyses how these ethical issues were perceived, detected and managed by the researchers, and the role of ethics review committees in mental health research concerning IDPs. The relevance of guidelines and methodologies in the context of an atypical study population and the benefit versus risk potential of research for IDPs are also discussed. The limitations that were encountered while dealing with ethical challenges during the study are discussed. The concept of post-research ethical conduct audit is suggested to be considered as a potential step to minimize the exploitation of vulnerable populations such as IDPs in mental health research. PMID:23497333

Intrinsically Disordered Protein Specific Force Field CHARMM36IDPSFF.

PubMed

Liu, Hao; Song, Dong; Lu, Hui; Luo, Ray; Chen, Hai-Feng

2018-05-28

Intrinsically disordered proteins (IDPs) are closely related to various human diseases. Because IDPs lack certain tertiary structure, it is difficult to use X-ray and NMR methods to measure their structures. Therefore, molecular dynamics simulation is a useful tool to study the conformer distribution of IDPs. However, most generic protein force fields were found to be insufficient in simulations of IDPs. Here we report our development for the CHARMM community. Our residue-specific IDP force field (CHARMM36IDPSFF) was developed based on the base generic force field with CMAP corrections of for all 20 naturally occurring amino acids. Multiple tests show that the simulated chemical shifts with the newly developed force field are in quantitative agreement with NMR experiment and are more accurate than the base generic force field. Comparison of J-couplings with previous work shows that CHARMM36IDPSFF and its corresponding base generic force field have their own advantages. In addition, CHARMM36IDPSFF simulations also agree with experiment for SAXS profiles and radii of gyration of IDPs. Detailed analysis shows that CHARMM36IDPSFF can sample more diverse and disordered conformers. These findings confirm that the newly developed force field can improve the balance of accuracy and efficiency for the conformer sampling of IDPs. This article is protected by copyright. All rights reserved. This article is protected by copyright. All rights reserved.

Ethical challenges in mental health research among internally displaced people: ethical theory and research implementation.

PubMed

Siriwardhana, Chesmal; Adikari, Anushka; Jayaweera, Kaushalya; Sumathipala, Athula

2013-03-12

Millions of people undergo displacement in the world. Internally displaced people (IDP) are especially vulnerable as they are not protected by special legislation in contrast to other migrants. Research conducted among IDPs must be correspondingly sensitive in dealing with ethical issues that may arise. Muslim IDPs in Puttalam district in the North-Western province of Sri Lanka were initially displaced from Northern Sri Lanka due to the conflict in 1991. In the backdrop of a study exploring the prevalence of common mental disorders among the IDPs, researchers encountered various ethical challenges. These included inter-related issues of autonomy, non-maleficence, beneficence, confidentiality and informed consent, and how these were tailored in a culture-specific way to a population that has increased vulnerability. This paper analyses how these ethical issues were perceived, detected and managed by the researchers, and the role of ethics review committees in mental health research concerning IDPs. The relevance of guidelines and methodologies in the context of an atypical study population and the benefit versus risk potential of research for IDPs are also discussed. The limitations that were encountered while dealing with ethical challenges during the study are discussed. The concept of post-research ethical conduct audit is suggested to be considered as a potential step to minimize the exploitation of vulnerable populations such as IDPs in mental health research.

The Joint Polar Satellite System (JPSS) Program's Algorithm Change Process (ACP): Past, Present and Future

NASA Technical Reports Server (NTRS)

Griffin, Ashley

2017-01-01

The Joint Polar Satellite System (JPSS) Program Office is the supporting organization for the Suomi National Polar Orbiting Partnership (S-NPP) and JPSS-1 satellites. S-NPP carries the following sensors: VIIRS, CrIS, ATMS, OMPS, and CERES with instruments that ultimately produce over 25 data products that cover the Earths weather, oceans, and atmosphere. A team of scientists and engineers from all over the United States document, monitor and fix errors in operational software code or documentation with the algorithm change process (ACP) to ensure the success of the S-NPP and JPSS 1 missions by maintaining quality and accuracy of the data products the scientific community relies on. This poster will outline the programs algorithm change process (ACP), identify the various users and scientific applications of our operational data products and highlight changes that have been made to the ACP to accommodate operating system upgrades to the JPSS programs Interface Data Processing Segment (IDPS), so that the program is ready for the transition to the 2017 JPSS-1 satellite mission and beyond.

Micro-analyses of Interplanetary Dust Particles (IDPs) and Micrometeorites (MMs): Implications for sample return missions to undifferentiated protoplanets

NASA Astrophysics Data System (ADS)

Rietmeijer, F.

The good news is that the original, typically non-chondritic, presolar dust had an extremely simple mineralogy of predominantly Mg-rich olivines and -pyroxenes, pyrrhotite (Fe7 S8 ), Fe-o xides and Fe,Ni-metal. This unique property is preserved in the least modified protoplanets for in situ sampling (e.g. STARDUST, MUSES-C) and in their debris in the form of stratospheric IDPs and MMs. The corollary is that mineralogical complexity in all extraterrestrial materials is an evolved secondary property. The earliest stages of solar system evolution were defined by hierarchical dust accretion whereby the accreting dust was recycled prior to the formation of the final surviving protoplanets. This recycling concentrated initially minor elements so they could form new minerals , e.g. alkali-feldspars and plagioclase. The least- modified protoplanets are comet nuclei, i.e. random mixtures of rubble piles and dirty snowballs, and the icy (ultra)carbonaceous asteroids. Second best are the dormant, extinct and rare active comet nuclei among the near-Earth asteroids that are relatively easy to access by sample return missions. Third are the anhydrous CO/CV carbonaceous chondrites and the low metamorphic grade, unequilibrated ordinary chondrites from the main asteroid belt. Lithification of the original rubble piles in these asteroids erased all structural properties but not the mineralogy and chemistry of the accreted entities, i.e. matrix, chondrules and CAIs.Consequently , returned samples of small chips, fragments or powders from the surface of undifferentiated protoplanets will amply suffice for a full mineralogical and chemical characterization of these small bodies, including modifications from interactions with the space environment, e.g. space weathering, regolith formation and the black mantle on icy-protoplanets. Major improvements in the sensitivity of available micro-analytical tools means that in situ acquired samples can be analyzed at scales of individual, n m-s i

Mortality and health among internally displaced persons in western Kenya following post-election violence, 2008: novel use of demographic surveillance

PubMed Central

Adazu, Kubaje; Obor, David; Ogwang, Sheila; Vulule, John; Hamel, Mary J; Laserson, Kayla

2010-01-01

Abstract Objective To evaluate mortality and morbidity among internally displaced persons (IDPs) who relocated in a demographic surveillance system (DSS) area in western Kenya following post-election violence. Methods In 2007, 204 000 individuals lived in the DSS area, where field workers visit households every 4 months to record migrations, births and deaths. We collected data on admissions among children < 5 years of age in the district hospital and developed special questionnaires to record information on IDPs. Mortality, migration and hospitalization rates among IDPs and regular DSS residents were compared, and verbal autopsies were performed for deaths. Findings Between December 2007 and May 2008, 16 428 IDPs migrated into the DSS, and over half of them stayed 6 months or longer. In 2008, IDPs aged 15–49 years died at higher rates than regular residents of the DSS (relative risk, RR: 1.34; 95% confidence interval, CI: 1.004–1.80). A greater percentage of deaths from human immunodeficiency virus (HIV) infection occurred among IDPs aged ≥ 5 years (53%) than among regular DSS residents (25–29%) (P < 0.001). Internally displaced children < 5 years of age did not die at higher rates than resident children but were hospitalized at higher rates (RR: 2.95; 95% CI: 2.44–3.58). Conclusion HIV-infected internally displaced adults in conflict-ridden parts of Africa are at increased risk of HIV-related death. Relief efforts should extend to IDPs who have relocated outside IDP camps, particularly if afflicted with HIV infection or other chronic conditions. PMID:20680125

Contribution of proline to the pre-structuring tendency of transient helical secondary structure elements in intrinsically disordered proteins.

PubMed

Lee, Chewook; Kalmar, Lajos; Xue, Bin; Tompa, Peter; Daughdrill, Gary W; Uversky, Vladimir N; Han, Kyou-Hoon

2014-03-01

IDPs function without relying on three-dimensional structures. No clear rationale for such a behavior is available yet. PreSMos are transient secondary structures observed in the target-free IDPs and serve as the target-binding "active" motifs in IDPs. Prolines are frequently found in the flanking regions of PreSMos. Contribution of prolines to the conformational stability of the helical PreSMos in IDPs is investigated. MD simulations are performed for several IDP segments containing a helical PreSMo and the flanking prolines. To measure the influence of flanking-prolines on the structural content of a helical PreSMo calculations were done for wild type as well as for mutant segments with Pro→Asp, His, Lys, or Ala. The change in the helicity due to removal of a proline was measured both for the PreSMo region and for the flanking regions. The α-helical content in ~70% of the helical PreSMos at the early stage of simulation decreases due to replacement of an N-terminal flanking proline by other residues whereas the helix content in nearly all PreSMos increases when the same replacements occur at the C-terminal flanking region. The helix destabilizing/terminating role of the C-terminal flanking prolines is more pronounced than the helix promoting effect of the N-terminal flanking prolines. This work represents a novel example demonstrating that a proline is encoded in an IDP with a defined purpose. The helical PreSMos presage their target-bound conformations. As they most likely mediate IDP-target binding via conformational selection their helical content can be an important feature for IDP function. Copyright © 2013 Elsevier B.V. All rights reserved.

NASA Science Data Processing for SNPP

NASA Astrophysics Data System (ADS)

Hall, A.; Behnke, J.; Lowe, D. R.; Ho, E. L.

2014-12-01

NASA's ESDIS Project has been operating the Suomi National Polar-Orbiting Partnership (SNPP) Science Data Segment (SDS) since the launch in October 2011. The science data processing system includes a Science Data Depository and Distribution Element (SD3E) and five Product Evaluation and Analysis Tool Elements (PEATEs): Land, Ocean, Atmosphere, Ozone, and Sounder. The SDS has been responsible for assessing Environmental Data Records (EDRs) for climate quality, providing and demonstrating algorithm improvements/enhancements and supporting the calibration/validation activities as well as instrument calibration and sensor table uploads for mission planning. The SNPP also flies two NASA instruments: OMPS Limb and CERES. The SNPP SDS has been responsible for producing, archiving and distributing the standard products for those instruments in close association with their NASA science teams. The PEATEs leveraged existing science data processing techniques developed under the EOSDIS Program. This enabled he PEATEs to do an excellent job in supporting Science Team analysis for SNPP. The SDS acquires data from three sources: NESDIS IDPS (Raw Data Records (RDRs)), GRAVITE (Retained Intermediate Products (RIPs)), and the NOAA/CLASS (higher level products). The SD3E component aggregates the RDRs, and distributes them to each of the PEATEs for further analysis and processing. It provides a ~32 day rolling storage of data, available for pickup by the PEATEs. The current system used by NASA will be presented along with plans for streamlining the system in support of continuing the NASA's EOS measurements.

Meteoric Material: An Important Component of Planetary Atmospheres

NASA Technical Reports Server (NTRS)

Grebowsky, Joseph M.; Moses, Julianne I.; Pesnell, W. Dean; Vondrak, Richard R. (Technical Monitor)

2001-01-01

Interplanetary dust particles (IDPs) interact with all planetary atmospheres and leave their imprint as perturbations of the background atmospheric chemistry and structure. They lead to layers of metal ions that can become the dominant positively charged species in lower ionospheric regions. Theoretical models and radio occultation measurements provide compelling evidence that such layers exist in all planetary atmospheres. In addition IDP ablation products can affect neutral atmospheric chemistry, particularly at the outer planets where the IDPs supply oxygen compounds like water and carbon dioxide to the upper atmospheres. Aerosol or smoke particles from incomplete ablation or recondensation of ablated IDP vapors may also have a significant impact on atmospheric properties.

Evidence-based diuretics: focus on chlorthalidone and indapamide.

PubMed

DiNicolantonio, James J; Bhutani, Jaikrit; Lavie, Carl J; O'Keefe, James H

2015-03-01

Thiazide and thiazide-like diuretics are cornerstone treatments for hypertension. However, unlike chlorthalidone (CTD) and indapamide (IDP), hydrochlorothiazide (HCTZ) lacks evidence for reducing morbidity and mortality as monotherapy compared with placebo or control. Despite this fact, HCTZ is prescribed much more frequently than CTD or IDP. We believe that all hypertension guidelines should follow the National Institute for Health and Excellence (NICE) and make IDP and CTD first choice 'thiazide-like diuretics.' This article will focus on the available evidence pertaining to HCTZ versus CTD and IDP. We will review the pharmacological differences between these three diuretics, as well as the clinical trial data and important side effects.

Satellite Capabilities Mapping - Utilizing Small Satellites

DTIC Science & Technology

2010-09-01

Metrics Definition…………………………..50 Figure 19. System and Requirements Decomposition…………………………………...59 Figure 20. TPS Fuctional Mapping Process...offered by small satellites. “The primary force in our corner of the universe is our sun. The sun is constantly radiating enormous amounts of...weather prediction models, a primary tool for forecasting weather” [19]. The NPOESS was a tri-agency program intended to develop and operate the next

Submicrometer Organic Grains: Widespread Constituents of the Early Solar System

NASA Technical Reports Server (NTRS)

Messenger, Scott; Nakamuri-Messenger, Keiko; Keller, Lindsay; Matrajt, Graciela; Clemett, Simon; Ito, Motoo

2007-01-01

Primitive meteorites and interplanetary dust particles (IDPs) contain remants of interstellar organic matter, marked by anomalous H and N isotopic ratios. These isotopic anomalies are attributed to mass fractionation during chemical reactions at cryogenic temperatures (10-100K) in a cold molecular cloud. Significant variations in the chemistry and isotopic compositions of organic compounds within and between these samples suggest varying histories of alteration and dilution of the presolar components. Recent studies have reported large H and N isotopic anomalies preserved in sub-m organic inclusions in both meteorites and IDPs. In the Tagish Lake meteorite, the largest H and N isotopic anomalies are associated with sub-m, hollow organic globules. The common physical, chemical, and isotopic characteristics of these globules suggest that they formed before being incorporated into their parent meteorite. These organic globules probably originated as organic ice coatings that formed on preexisting ice or mineral grains in a cold molecular cloud. Radiation driven photochemistry may have processed them into refractory organic grains. This model implies that submicrometer organic grains were widely distributed throughout the solar nebula during the epoch of planet formation. Submicrometer organic particles were detected by the Giotto and Vega encounters with comet Halley, termed CHON particles based on their major element chemistry. The first direct samples of cometary dust (comet Wild-2) were returned by the Stardust spacecraft in January 2006. These samples exhibit widely varying, fine grained mineralogy similar to anhydrous IDPs, including submicrometer carbonaceous grains. The submicrometer organic grains from comet Wild-2 exhibit H and N isotopic anomalies of similar magnitude to those commonly observed in primitive meteorites and IDPs. Isotopically anomalous, submicrometer organic grains have now been observed in meteorites, IDPs, the Oort-cloud comet Halley, and

Unusual biophysics of intrinsically disordered proteins.

PubMed

Uversky, Vladimir N

2013-05-01

Research of a past decade and a half leaves no doubt that complete understanding of protein functionality requires close consideration of the fact that many functional proteins do not have well-folded structures. These intrinsically disordered proteins (IDPs) and proteins with intrinsically disordered protein regions (IDPRs) are highly abundant in nature and play a number of crucial roles in a living cell. Their functions, which are typically associated with a wide range of intermolecular interactions where IDPs possess remarkable binding promiscuity, complement functional repertoire of ordered proteins. All this requires a close attention to the peculiarities of biophysics of these proteins. In this review, some key biophysical features of IDPs are covered. In addition to the peculiar sequence characteristics of IDPs these biophysical features include sequential, structural, and spatiotemporal heterogeneity of IDPs; their rough and relatively flat energy landscapes; their ability to undergo both induced folding and induced unfolding; the ability to interact specifically with structurally unrelated partners; the ability to gain different structures at binding to different partners; and the ability to keep essential amount of disorder even in the bound form. IDPs are also characterized by the "turned-out" response to the changes in their environment, where they gain some structure under conditions resulting in denaturation or even unfolding of ordered proteins. It is proposed that the heterogeneous spatiotemporal structure of IDPs/IDPRs can be described as a set of foldons, inducible foldons, semi-foldons, non-foldons, and unfoldons. They may lose their function when folded, and activation of some IDPs is associated with the awaking of the dormant disorder. It is possible that IDPs represent the "edge of chaos" systems which operate in a region between order and complete randomness or chaos, where the complexity is maximal. This article is part of a Special Issue

Hygienic practices and diarrheal illness among persons living in at-risk settings in Kabul, Afghanistan: a cross-sectional study.

PubMed

Mubarak, Mohammad Yousuf; Wagner, Abram L; Asami, Mari; Carlson, Bradley F; Boulton, Matthew L

2016-08-31

Sustained civil and military conflict, resulting in large numbers of internally displaced persons (IDP), in combination with rapid urbanization has strained public health and sanitation within cities in Afghanistan. In order to examine the association between preventive sanitary behaviors and diarrhea within two high risk settings located within Kabul, Afghanistan, this study aimed to evaluate the prevalence of hygienic practices and diarrheal illness in an IDP camp and an urban slum. In this cross sectional study, a convenience sample of residents of an IDP camp and an urban slum in Kabul, Afghanistan, was used. Participants were asked to describe their hygienic practices and interviewers independently documented household sanitation. The knowledge and attitudes about and practice of hygienic activities to prevent diarrhea were compared between the two settings. Two hundred participants, 100 from each setting, were enrolled. Knowledge, attitudes, and practices regarding hygienic activities to prevent diarrhea were greater among the slum dwellers than the IDP. Fewer than half of participants washed their hands with soap before eating or after eating: 31 % of slum dwellers washed before eating compared to 11 % of IDPs (P = 0.0050), and 25 % of slum dwellers washed after defecating compared to 4 % of IDPs (P = 0.0020). The IDPs were more likely to share a latrine (P = 0.0144) and less likely to disinfect their latrine than slum dwellers. Diarrhea in the household within the past 3 months was more common in the IDP camp (54 %) than the slum (20 %) (P = 0.0020). Even though certain sanitary and hygienic practices were more common among slum dwellers than IDPs, the lack of hygienic activities in both setting indicates that interventions to change behavior, like increasing the availability of soap and encouraging hand washing, are needed. Any initiative will have to be developed in the context of pervasive illiteracy among persons in both of these

The mental health, quality of life and life satisfaction of internally displaced persons living in Nakuru County, Kenya.

PubMed

Getanda, Elijah Mironga; Papadopoulos, Chris; Evans, Hala

2015-08-06

Internally displaced persons (IDPs) are among the most vulnerable people in the world today. Previous research highlights that conflict-induced forced displacement can cause problems with mental health and wellbeing. This study aimed to contribute to this body of knowledge by investigating the mental health, quality of life, and life satisfaction among IDPs living in Nakuru, Kenya. A questionnaire that included the General Health Questionnaire-12, Satisfaction with Life Scale, and a modified version of the WHO Quality of Life-BREF tool was used for data collection. The questionnaire also included an open-ended question inviting qualitative responses about their experience as an IDP. The questionnaire was distributed through a three-stage sampling approach across four refugee camps from four regions of the Nakuru County in Kenya. One hundred IDPs participated in this study. All participants scored substantially higher than the applied GHQ-12 threshold for caseness (mean GHQ-12 score = 28.7, SD = 3.6). Quality of life and life satisfaction scores were also very poor (M = 10.24, SD = 1.9; M = 6.82, SD = 1.5 respectively). The qualitative results reflected these findings with statements reflecting suicidal thoughts, unhappiness with the government, lack of support, and fear for themselves and their children. Significantly higher GHQ-12 scores were found among older IDPs (rho = .202, sig = .046), widowers compared to married IDPs (mean difference = -2.41, SE = .885, sig = .027), while lower scores were found among IDPs who reported having friends as a source of support (U = 834, sig = .045), while quality of life scores were higher among IDPs who reported receiving governmental support (U = 248, sig = .018). The findings revealed poor levels of mental health, quality of life and life satisfaction. Older, widowed IDPs and those who did not perceive support from friends or the government were found to be at the highest risk of poor health and wellbeing.

Sub-Micrometer Scale Minor Element Mapping in Interplanetary Dust Particles: A Test for Stratospheric Contamination

NASA Technical Reports Server (NTRS)

Flynn, G. J.; Keller, L. P.; Sutton, S. R.

2004-01-01

Combined X-ray microprobe (XRM), energy dispersive x-ray fluorescence using a Transmission Electron Microscope (TEM), and electron microprobe measurements have determined that the average bulk chemical composition of the interplanetary dust particles (IDPs) collected from the Earth s stratosphere is enriched relative to the CI meteorite composition by a factor of 2 to 4 for carbon and for the moderately volatile elements Na, K, P, Mn, Cu, Zn, Ga, Ge, and Se, and enriched to approximately 30 times CI for Br. However, Jessberger et al., who have reported similar bulk enrichments using Proton Induced X-ray Emission (PIXE), attribute the enrichments to contamination by meteor-derived atmospheric aerosols during the several weeks these IDPs reside in the Earth s atmosphere prior to collection. Using scanning Auger spectroscopy, a very sensitive surface analysis technique, Mackinnon and Mogk have observed S contamination on the surface of IDPs, presumably due to the accretion of sulfate aerosols during stratospheric residence. But the S-rich layer they detected was so thin (approximately 100 angstroms thick) that the total amount of S on the surface was too small to significantly perturb the bulk S-content of a chondritic IDP. Stephan et al. provide support for the contamination hypothesis by reporting the enrichment of Br on the edges of the IDPs using Time-of-Flight Secondary-Ion Mass-Spectrometry (TOFSIMS), but TOF-SIMS is notorious for producing false edge-effects, particularly on irregularly-shaped samples like IDPs. Sutton et al. mapped the spatial distribution of Fe, Ni, Zn, Br, and Sr, at the approximately 2 m scale, in four IDPs using element-specific x-ray fluorescence (XRF) computed microtomography. They found the moderately volatile elements Zn and Br, although spatially inhomogeneous, were not concentrated on the surface of any of the IDPs they examined, suggesting that the Zn and the Br enrichments in the IDPs are not due to contamination during

Computer Simulations of Intrinsically Disordered Proteins

NASA Astrophysics Data System (ADS)

Chong, Song-Ho; Chatterjee, Prathit; Ham, Sihyun

2017-05-01

The investigation of intrinsically disordered proteins (IDPs) is a new frontier in structural and molecular biology that requires a new paradigm to connect structural disorder to function. Molecular dynamics simulations and statistical thermodynamics potentially offer ideal tools for atomic-level characterizations and thermodynamic descriptions of this fascinating class of proteins that will complement experimental studies. However, IDPs display sensitivity to inaccuracies in the underlying molecular mechanics force fields. Thus, achieving an accurate structural characterization of IDPs via simulations is a challenge. It is also daunting to perform a configuration-space integration over heterogeneous structural ensembles sampled by IDPs to extract, in particular, protein configurational entropy. In this review, we summarize recent efforts devoted to the development of force fields and the critical evaluations of their performance when applied to IDPs. We also survey recent advances in computational methods for protein configurational entropy that aim to provide a thermodynamic link between structural disorder and protein activity.

The sexual and reproductive rights of internally displaced women: the embodiment of Colombia's crisis.

PubMed

Alzate, Mónica M

2008-03-01

As of 30 June 2006, more than 3.5 million Colombians are internally displaced persons (IDPs), the second largest IDP population in the world after that of Sudan. While most IDP studies treat the plight of internally displaced women (IDW) as an isolated phenomenon, this paper demonstrates that their situation reflects Colombia's chronic cultural, political and socio-economic crisis. This paper uses a sexual and reproductive rights framework to establish a connection between IDW and Colombia's culture of violence, discrimination and inequality. The effects of this culture of violence, discrimination and inequality are highlighted during a discussion of the rights to health, reproduction, privacy, physical integrity, education, and freedom from violence and sexual exploitation. This paper argues that a holistic understanding of Colombia's humanitarian emergency is essential to improving the lives of IDPs. It ends with some concrete, short-term recommendations to meet some of the needs of IDPs and other vulnerable populations.

Molecular Dynamics Simulations of Intrinsically Disordered Proteins: On the Accuracy of the TIP4P-D Water Model and the Representativeness of Protein Disorder Models.

PubMed

Henriques, João; Skepö, Marie

2016-07-12

Here, we first present a follow-up to a previous work by our group on the problematic of molecular dynamics simulations of intrinsically disordered proteins (IDPs) [ Henriques et al. J. Chem. Theory Comput. 2015 , 11 , 3420 - 3431 ], using the recently developed TIP4P-D water model. When used in conjunction with the standard AMBER ff99SB-ILDN force field and applied to the simulation of Histatin 5, our IDP model, we obtain results which are in excellent agreement with the best performing IDP-suitable force field from the earlier study and with experiment. We then assess the representativeness of the IDP models used in these and similar studies, finding that most are too short in comparison to the average IDP and contain a bias toward hydrophilic amino acid residues. Moreover, several key order- and disorder-promoting residues are also found to be misrepresented. It seems appropriate for future studies to address these issues.

Wrecked regulation of intrinsically disordered proteins in diseases: pathogenicity of deregulated regulators

PubMed Central

Uversky, Vladimir N.

2014-01-01

Biologically active proteins without stable tertiary structure are common in all known proteomes. Functions of these intrinsically disordered proteins (IDPs) are typically related to regulation, signaling, and control. Cellular levels of these important regulators are tightly regulated by a variety mechanisms ranging from firmly controlled expression to precisely targeted degradation. Functions of IDPs are controlled by binding to specific partners, alternative splicing, and posttranslational modifications among other means. In the norm, right amounts of precisely activated IDPs have to be present in right time at right places. Wrecked regulation brings havoc to the ordered world of disordered proteins, leading to protein misfolding, misidentification, and missignaling that give rise to numerous human diseases, such as cancer, cardiovascular disease, neurodegenerative diseases, and diabetes. Among factors inducing pathogenic transformations of IDPs are various cellular mechanisms, such as chromosomal translocations, damaged splicing, altered expression, frustrated posttranslational modifications, aberrant proteolytic degradation, and defective trafficking. This review presents some of the aspects of deregulated regulation of IDPs leading to human diseases. PMID:25988147

Internal displacement and health among the Palestinian minority in Israel.

PubMed

Daoud, Nihaya; Shankardass, Ketan; O'Campo, Patricia; Anderson, Kim; Agbaria, Ayman K

2012-04-01

Long term health impacts of internal displacement (ID) resulting from political violence are not well documented or understood. One such case is the ID of 300,000-420,000 Palestinian citizens of Israel and their descendants during the Nakba of 1948 (Palestinian Catastrophe). We aim to document the long term health impacts of this ID. We draw on data collected in 2005 from a nationwide random sample of 902 individuals aged 30-70. Research participants were interviewed in person after being selected through a multistage sampling procedure. About 24% of participants reported that either they or their families had been internally displaced. Palestinian internally displaced persons (IDPs), that is, those who were forcibly displaced and dispossessed from their homes and lands during the Nakba and its aftermath, as well as their families and descendants, and who reside within the current borders of Israel, had an odds ratio of 1.45 (95% CI = 1.02-2.07) for poor self-rated health (SRH) compared to non-IDPs after controlling for demographic, socioeconomic and psychosocial factors. No difference was found between IDPs and non-IDPs in limiting longstanding illness following control for confounders. Low socioeconomic position and chronic stress were significantly related to ID and to SRH. Our findings suggest adverse long term health impacts of the Nakba on the IDPs when compared to non-IDPs. We propose that these disparities might stem from IDPs' unhealed post-traumatic scars from the Nakba, or from becoming a marginalized minority within their own society due to their displacement and loss of collective identity. Given these long term health consequences, we conclude that displacement should be addressed with health and social policies for IDPs. Copyright © 2012 Elsevier Ltd. All rights reserved.

GeoInt: the first macroseismic intensity database for the Republic of Georgia

NASA Astrophysics Data System (ADS)

Varazanashvili, O.; Tsereteli, N.; Bonali, F. L.; Arabidze, V.; Russo, E.; Pasquaré Mariotto, F.; Gogoladze, Z.; Tibaldi, A.; Kvavadze, N.; Oppizzi, P.

2018-05-01

Our work is intended to present the new macroseismic intensity database for the Republic of Georgia—hereby named GeoInt—which includes earthquakes from the historical (from 1250 B.C. onwards) to the instrumental era. Such database is composed of 111 selected earthquakes and related 3944 intensity data points (IDPs) for 1509 different localities, reported in the Medvedev-Sponheuer-Karnik scale (MSK). Regarding the earthquakes, the M S is in the 3.3-7 range and the depth is in the 2-36 km range. The entire set of IDPs is characterized by intensities ranging from 2-3 to 9-10 and covers an area spanning from 39.508° N to 45.043° N in a N-S direction and from 37.324° E to 48.500° E in an E-W direction, with some of the IDPs located outside the Georgian border, in the (i) Republic of Armenia, (ii) Russian Federation, (iii) Republic of Turkey, and (iv) Republic of Azerbaijan. We have revised each single IDP and have reevaluated and homogenized intensity values to the MSK scale. In particular, regarding the whole set of 3944 IDPs, 348 belong to the Historical era (pre-1900) and 3596 belong to the instrumental era (post-1900). With particular regard to the 3596 IDPs, 105 are brand new (3%), whereas the intensity values for 804 IDPs have been reevaluated (22%); for 2687 IDPs (75%), intensities have been confirmed from previous interpretations. We introduce this database as a key input for further improvements in seismic hazard modeling and seismic risk calculation for this region, based on macroseismic intensity; we report all the 111 earthquakes with available macroseismic information. The GeoInt database is also accessible online at http://www.enguriproject.unimib.it and will be kept updated in the future.

Electrostatically Accelerated Encounter and Folding for Facile Recognition of Intrinsically Disordered Proteins

PubMed Central

Ganguly, Debabani; Zhang, Weihong; Chen, Jianhan

2013-01-01

Achieving facile specific recognition is essential for intrinsically disordered proteins (IDPs) that are involved in cellular signaling and regulation. Consideration of the physical time scales of protein folding and diffusion-limited protein-protein encounter has suggested that the frequent requirement of protein folding for specific IDP recognition could lead to kinetic bottlenecks. How IDPs overcome such potential kinetic bottlenecks to viably function in signaling and regulation in general is poorly understood. Our recent computational and experimental study of cell-cycle regulator p27 (Ganguly et al., J. Mol. Biol. (2012)) demonstrated that long-range electrostatic forces exerted on enriched charges of IDPs could accelerate protein-protein encounter via “electrostatic steering” and at the same time promote “folding-competent” encounter topologies to enhance the efficiency of IDP folding upon encounter. Here, we further investigated the coupled binding and folding mechanisms and the roles of electrostatic forces in the formation of three IDP complexes with more complex folded topologies. The surface electrostatic potentials of these complexes lack prominent features like those observed for the p27/Cdk2/cyclin A complex to directly suggest the ability of electrostatic forces to facilitate folding upon encounter. Nonetheless, similar electrostatically accelerated encounter and folding mechanisms were consistently predicted for all three complexes using topology-based coarse-grained simulations. Together with our previous analysis of charge distributions in known IDP complexes, our results support a prevalent role of electrostatic interactions in promoting efficient coupled binding and folding for facile specific recognition. These results also suggest that there is likely a co-evolution of IDP folded topology, charge characteristics, and coupled binding and folding mechanisms, driven at least partially by the need to achieve fast association kinetics for

Generating intrinsically disordered protein conformational ensembles from a Markov chain

NASA Astrophysics Data System (ADS)

Cukier, Robert I.

2018-03-01

Intrinsically disordered proteins (IDPs) sample a diverse conformational space. They are important to signaling and regulatory pathways in cells. An entropy penalty must be payed when an IDP becomes ordered upon interaction with another protein or a ligand. Thus, the degree of conformational disorder of an IDP is of interest. We create a dichotomic Markov model that can explore entropic features of an IDP. The Markov condition introduces local (neighbor residues in a protein sequence) rotamer dependences that arise from van der Waals and other chemical constraints. A protein sequence of length N is characterized by its (information) entropy and mutual information, MIMC, the latter providing a measure of the dependence among the random variables describing the rotamer probabilities of the residues that comprise the sequence. For a Markov chain, the MIMC is proportional to the pair mutual information MI which depends on the singlet and pair probabilities of neighbor residue rotamer sampling. All 2N sequence states are generated, along with their probabilities, and contrasted with the probabilities under the assumption of independent residues. An efficient method to generate realizations of the chain is also provided. The chain entropy, MIMC, and state probabilities provide the ingredients to distinguish different scenarios using the terminologies: MoRF (molecular recognition feature), not-MoRF, and not-IDP. A MoRF corresponds to large entropy and large MIMC (strong dependence among the residues' rotamer sampling), a not-MoRF corresponds to large entropy but small MIMC, and not-IDP corresponds to low entropy irrespective of the MIMC. We show that MorFs are most appropriate as descriptors of IDPs. They provide a reasonable number of high-population states that reflect the dependences between neighbor residues, thus classifying them as IDPs, yet without very large entropy that might lead to a too high entropy penalty.

GeoInt: the first macroseismic intensity database for the Republic of Georgia

NASA Astrophysics Data System (ADS)

Varazanashvili, O.; Tsereteli, N.; Bonali, F. L.; Arabidze, V.; Russo, E.; Pasquaré Mariotto, F.; Gogoladze, Z.; Tibaldi, A.; Kvavadze, N.; Oppizzi, P.

2018-01-01

Our work is intended to present the new macroseismic intensity database for the Republic of Georgia—hereby named GeoInt—which includes earthquakes from the historical (from 1250 B.C. onwards) to the instrumental era. Such database is composed of 111 selected earthquakes and related 3944 intensity data points (IDPs) for 1509 different localities, reported in the Medvedev-Sponheuer-Karnik scale (MSK). Regarding the earthquakes, the M S is in the 3.3-7 range and the depth is in the 2-36 km range. The entire set of IDPs is characterized by intensities ranging from 2-3 to 9-10 and covers an area spanning from 39.508° N to 45.043° N in a N-S direction and from 37.324° E to 48.500° E in an E-W direction, with some of the IDPs located outside the Georgian border, in the (i) Republic of Armenia, (ii) Russian Federation, (iii) Republic of Turkey, and (iv) Republic of Azerbaijan. We have revised each single IDP and have reevaluated and homogenized intensity values to the MSK scale. In particular, regarding the whole set of 3944 IDPs, 348 belong to the Historical era (pre-1900) and 3596 belong to the instrumental era (post-1900). With particular regard to the 3596 IDPs, 105 are brand new (3%), whereas the intensity values for 804 IDPs have been reevaluated (22%); for 2687 IDPs (75%), intensities have been confirmed from previous interpretations. We introduce this database as a key input for further improvements in seismic hazard modeling and seismic risk calculation for this region, based on macroseismic intensity; we report all the 111 earthquakes with available macroseismic information. The GeoInt database is also accessible online at http://www.enguriproject.unimib.it and will be kept updated in the future.

AIRS Retrieval Validation During the EAQUATE

NASA Technical Reports Server (NTRS)

Zhou, Daniel K.; Smith, William L.; Cuomo, Vincenzo; Taylor, Jonathan P.; Barnet, Christopher D.; DiGirolamo, Paolo; Pappalardo, Gelsomina; Larar, Allen M.; Liu, Xu; Newman, Stuart M.

2006-01-01

Atmospheric and surface thermodynamic parameters retrieved with advanced hyperspectral remote sensors of Earth observing satellites are critical for weather prediction and scientific research. The retrieval algorithms and retrieved parameters from satellite sounders must be validated to demonstrate the capability and accuracy of both observation and data processing systems. The European AQUA Thermodynamic Experiment (EAQUATE) was conducted mainly for validation of the Atmospheric InfraRed Sounder (AIRS) on the AQUA satellite, but also for assessment of validation systems of both ground-based and aircraft-based instruments which will be used for other satellite systems such as the Infrared Atmospheric Sounding Interferometer (IASI) on the European MetOp satellite, the Cross-track Infrared Sounder (CrIS) from the NPOESS Preparatory Project and the following NPOESS series of satellites. Detailed inter-comparisons were conducted and presented using different retrieval methodologies: measurements from airborne ultraspectral Fourier transform spectrometers, aircraft in-situ instruments, dedicated dropsondes and radiosondes, and ground based Raman Lidar, as well as from the European Center for Medium range Weather Forecasting (ECMWF) modeled thermal structures. The results of this study not only illustrate the quality of the measurements and retrieval products but also demonstrate the capability of these validation systems which are put in place to validate current and future hyperspectral sounding instruments and their scientific products.

Consequences of inducing intrinsic disorder in a high-affinity protein-protein interaction.

PubMed

Papadakos, Grigorios; Sharma, Amit; Lancaster, Lorna E; Bowen, Rebecca; Kaminska, Renata; Leech, Andrew P; Walker, Daniel; Redfield, Christina; Kleanthous, Colin

2015-04-29

The kinetic and thermodynamic consequences of intrinsic disorder in protein-protein recognition are controversial. We address this by inducing one partner of the high-affinity colicin E3 rRNase domain-Im3 complex (K(d) ≈ 10(-12) M) to become an intrinsically disordered protein (IDP). Through a variety of biophysical measurements, we show that a single alanine mutation at Tyr507 within the hydrophobic core of the isolated colicin E3 rRNase domain causes the enzyme to become an IDP (E3 rRNase(IDP)). E3 rRNase(IDP) binds stoichiometrically to Im3 and forms a structure that is essentially identical to the wild-type complex. However, binding of E3 rRNase(IDP) to Im3 is 4 orders of magnitude weaker than that of the folded rRNase, with thermodynamic parameters reflecting the disorder-to-order transition on forming the complex. Critically, pre-steady-state kinetic analysis of the E3 rRNase(IDP)-Im3 complex demonstrates that the decrease in affinity is mostly accounted for by a drop in the electrostatically steered association rate. Our study shows that, notwithstanding the advantages intrinsic disorder brings to biological systems, this can come at severe kinetic and thermodynamic cost.

Molecular inference of sources and spreading patterns of Plasmodium falciparum malaria parasites in internally displaced persons settlements in Myanmar-China border area.

PubMed

Lo, Eugenia; Zhou, Guofa; Oo, Winny; Lee, Ming-Chieh; Baum, Elisabeth; Felgner, Philip L; Yang, Zhaoqing; Cui, Liwang; Yan, Guiyun

2015-07-01

In Myanmar, civil unrest and establishment of internally displaced persons (IDP) settlement along the Myanmar-China border have impacted malaria transmission. The growing IDP populations raise deep concerns about health impact on local communities. Microsatellite markers were used to examine the source and spreading patterns of Plasmodium falciparum between IDP settlement and surrounding villages in Myanmar along the China border. Genotypic structure of P. falciparum was compared over the past three years from the same area and the demographic history was inferred to determine the source of recent infections. In addition, we examined if border migration is a factor of P. falciparum infections in China by determining gene flow patterns across borders. Compared to local community, the IDP samples showed a reduced and consistently lower genetic diversity over the past three years. A strong signature of genetic bottleneck was detected in the IDP samples. P. falciparum infections from the border regions in China were genetically similar to Myanmar and parasite gene flow was not constrained by geographical distance. Reduced genetic diversity of P. falciparum suggested intense malaria control within the IDP settlement. Human movement was a key factor to the spread of malaria both locally in Myanmar and across the international border. Copyright © 2015 Elsevier B.V. All rights reserved.

Intradiscal Pressure Changes during Manual Cervical Distraction: A Cadaveric Study

PubMed Central

Gudavalli, M. R.; Potluri, T.; Carandang, G.; Havey, R. M.; Voronov, L. I.; Cox, J. M.; Rowell, R. M.; Kruse, R. A.; Joachim, G. C.; Patwardhan, A. G.; Henderson, C. N. R.; Goertz, C.

2013-01-01

The objective of this study was to measure intradiscal pressure (IDP) changes in the lower cervical spine during a manual cervical distraction (MCD) procedure. Incisions were made anteriorly, and pressure transducers were inserted into each nucleus at lower cervical discs. Four skilled doctors of chiropractic (DCs) performed MCD procedure on nine specimens in prone position with contacts at C5 or at C6 vertebrae with the headpiece in different positions. IDP changes, traction forces, and manually applied posterior-to-anterior forces were analyzed using descriptive statistics. IDP decreases were observed during MCD procedure at all lower cervical levels C4-C5, C5-C6, and C6-C7. The mean IDP decreases were as high as 168.7 KPa. Mean traction forces were as high as 119.2 N. Posterior-to-anterior forces applied during manual traction were as high as 82.6 N. Intraclinician reliability for IDP decrease was high for all four DCs. While two DCs had high intraclinician reliability for applied traction force, the other two DCs demonstrated only moderate reliability. IDP decreases were greatest during moving flexion and traction. They were progressevely less pronouced with neutral traction, fixed flexion and traction, and generalized traction. PMID:24023587

Suomi NPP VIIRS active fire product status

NASA Astrophysics Data System (ADS)

Ellicott, E. A.; Csiszar, I. A.; Schroeder, W.; Giglio, L.; Wind, B.; Justice, C. O.

2012-12-01

We provide an overview of the evaluation and development of the Active Fires product derived from the Visible Infrared Imager Radiometer Suite (VIIRS) sensor on the Suomi National Polar-orbiting Partnership (SNPP) satellite during the first year of on-orbit data. Results from the initial evaluation of the standard SNPP Active Fires product, generated by the SNPP Interface Data Processing System (IDPS), supported the stabilization of the VIIRS Sensor Data Record (SDR) product. This activity focused in particular on the processing of the dual-gain 4 micron VIIRS M13 radiometric measurements into 750m aggregated data, which are fundamental for active fire detection. Following the VIIRS SDR product's Beta maturity status in April 2012, correlative analysis between VIIRS and near-simultaneous fire detections from the Moderate Resolution Imaging Spectroradiometer (MODIS) on the NASA Earth Observing System Aqua satellite confirmed the expected relative detection rates driven primarily by sensor differences. The VIIRS Active Fires Product Development and Validation Team also developed a science code that is based on the latest MODIS Collection 6 algorithm and provides a full spatially explicit fire mask to replace the sparse array output of fire locations from a MODIS Collection 4 equivalent algorithm in the current IDPS product. The Algorithm Development Library (ADL) was used to support the planning for the transition of the science code into IDPS operations in the future. Product evaluation and user outreach was facilitated by a product website that provided end user access to fire data in user-friendly format over North America as well as examples of VIIRS-MODIS comparisons. The VIIRS fire team also developed an experimental product based on 375m VIIRS Imagery band measurements and provided high quality imagery of major fire events in US. By August 2012 the IDPS product achieved Beta maturity, with some known and documented shortfalls related to the processing of

Benign Intraductal Papilloma without Atypia on Core Needle Biopsy Has a Low Rate of Upgrading to Malignancy after Excision

PubMed Central

Han, Song-Hee; Kim, Milim; Chung, Yul Ri; Yun, Bo La; Jang, Mijung; Kim, Sun Mi; Kang, Eunyoung; Kim, Eun-Kyu

2018-01-01

Purpose The management of benign intraductal papilloma (IDP) without atypia diagnosed on core needle biopsy (CNB) remains controversial. This study was performed to evaluate the rate of upgrading to malignancy or high-risk lesions after excision and to identify factors associated with upgrading using a large series of benign IDP cases without atypia. Methods We included patients who were diagnosed as having benign IDP without atypia on CNB and underwent surgical or vacuum-assisted excision between 2010 and 2015. We analyzed the clinical, radiologic, and histopathologic features of IDPs that were upgraded to malignancy or high-risk lesions after excision. Results A total of 511 benign IDPs without atypia diagnosed via CNB were identified, of which 398 cases were treated with excision. After reviewing these cases, four cases of high-risk lesions in adjacent tissue on CNB, two cases which were revealed as papilloma with atypia, and nine cases of malignancy in the same breast were excluded. In the remaining 383 cases, the rate of upgrading to malignancy and high-risk lesions after excision was 0.8% and 4.4%, respectively. The presence of concurrent contralateral breast cancer, the presence of symptoms, and multifocality were factors significantly associated with upgrading to malignancy on subsequent excision. Surgical excision rather than vacuum-assisted excision was significantly associated with upgrading to high-risk lesions or malignancy. Conclusion The rate of upgrading to malignancy for benign IDP without atypia was very low, suggesting that close clinical and radiologic observation may be sufficient for patients with benign IDP without atypia on CNB under proper settings. PMID:29628987

Benign Intraductal Papilloma without Atypia on Core Needle Biopsy Has a Low Rate of Upgrading to Malignancy after Excision.

PubMed

Han, Song-Hee; Kim, Milim; Chung, Yul Ri; Yun, Bo La; Jang, Mijung; Kim, Sun Mi; Kang, Eunyoung; Kim, Eun-Kyu; Park, So Yeon

2018-03-01

The management of benign intraductal papilloma (IDP) without atypia diagnosed on core needle biopsy (CNB) remains controversial. This study was performed to evaluate the rate of upgrading to malignancy or high-risk lesions after excision and to identify factors associated with upgrading using a large series of benign IDP cases without atypia. We included patients who were diagnosed as having benign IDP without atypia on CNB and underwent surgical or vacuum-assisted excision between 2010 and 2015. We analyzed the clinical, radiologic, and histopathologic features of IDPs that were upgraded to malignancy or high-risk lesions after excision. A total of 511 benign IDPs without atypia diagnosed via CNB were identified, of which 398 cases were treated with excision. After reviewing these cases, four cases of high-risk lesions in adjacent tissue on CNB, two cases which were revealed as papilloma with atypia, and nine cases of malignancy in the same breast were excluded. In the remaining 383 cases, the rate of upgrading to malignancy and high-risk lesions after excision was 0.8% and 4.4%, respectively. The presence of concurrent contralateral breast cancer, the presence of symptoms, and multifocality were factors significantly associated with upgrading to malignancy on subsequent excision. Surgical excision rather than vacuum-assisted excision was significantly associated with upgrading to high-risk lesions or malignancy. The rate of upgrading to malignancy for benign IDP without atypia was very low, suggesting that close clinical and radiologic observation may be sufficient for patients with benign IDP without atypia on CNB under proper settings.

Tin in a chondritic interplanetary dust particle

NASA Technical Reports Server (NTRS)

Rietmeijer, Frans J. M.

1989-01-01

Submicron platey Sn-rich grains are present in chondritic porous interplanetary dust particle (IDP) W7029 A and it is the second occurrence of a tin mineral in a stratospheric micrometeorite. Selected Area Electron Diffraction data for the Sn-rich grains match with Sn2O3 and Sn3O4. The oxide(s) may have formed in the solar nebula when tin metal catalytically supported reduction of CO or during flash heating on atmospheric entry of the IDP. The presence of tin is consistent with enrichments for other volatile trace elements in chondritic IDPs and may signal an emerging trend toward nonchondritic volatile element abundances in chondritic IDPs. The observation confirms small-scale mineralogical heterogeneity in fine-grained chondritic porous interplanetary dust.

Acid dissolution experiments - Carbonates and the 6.8-micrometer bands in interplanetary dust particles

NASA Technical Reports Server (NTRS)

Sandford, S. A.

1986-01-01

A chemical dissolution experiment on an interplanetary dust particle (IDP) showed that carbonates, not acid-insoluble organic compounds, were responsible for virtually all the absorption at 6.8 micrometers seen in the infrared spectra of this particle. The IDP examined had an infrared spectrum characteristic of layer-lattice silicates and belongs to a class of IDP's whose spectra resemble those of protostellar objects like W33 A, which also exhibit a band at 6.8 micrometers.

Inclusion of climate change strategies in municipal Integrated Development Plans: A case from seven municipalities in Limpopo Province, South Africa

PubMed Central

2016-01-01

The Intergovernmental Panel on Climate Change (IPCC) has made it clear that anthropogenic greenhouse gasses are the main cause of observed global warming that leads to climate change. Climate change is now a global reality. In the South African political set-up, local municipalities are the structures that are in direct contact with communities and they draw up Integrated Development Plans (IDPs), which are reviewed and upgraded annually. The article seeks to investigate the extent to which climate change adaptation and mitigation strategies are embedded IDPs in seven vulnerable municipalities in the Limpopo Province. The article conducted an in-depth content analysis of the IDPs of the seven municipalities and the results have revealed that these municipalities have not included adaptation and mitigation strategies adequately in their IDPs despite being the most vulnerable municipalities in the province. The article concludes that these municipalities have not as yet institutionalised climate change in their daily operations, planning and decision making. To this end, the paper recommends that local municipalities should include climate change adaptation and mitigation strategies in their IDPs.

Forced migration and mental health: prolonged internal displacement, return migration and resilience.

PubMed

Siriwardhana, Chesmal; Stewart, Robert

2013-03-01

Forced internal displacement has been rising steadily, mainly due to conflict. Many internally displaced people (IDP) experience prolonged displacement. Global research evidence suggests that many of these IDP are at high risk for developing mental disorders, adding weight to the global burden of disease. However, individual and community resilience may act as protective factors. Return migration may be an option for some IDP populations, especially when conflicts end, although return migration may itself be associated with worse mental health. Limited evidence is available on effects of resettlement or return migration following prolonged forced internal displacement on mental health. Also, the role of resilience factors remains to be clarified following situations of prolonged displacement. The public health impact of internal displacement is not clearly understood. Epidemiological and interventional research in IDP mental health needs to look beyond medicalised models and encompass broader social and cultural aspects. The resilience factor should be integrated and explored more in mental health research among IDP and a clearly focused multidisciplinary approach is advocated.

Maternal and Child Health of Internally Displaced Persons in Ukraine: A Qualitative Study

PubMed Central

Nidzvetska, Svitlana; Rodriguez-Llanes, Jose M.; Aujoulat, Isabelle; Gil Cuesta, Julita; Tappis, Hannah; van Loenhout, Joris A. F.; Guha-Sapir, Debarati

2017-01-01

Due to the conflict that started in spring 2014 in Eastern Ukraine, a total of 1.75 million internally displaced persons (IDPs) fled the area and have been registered in government-controlled areas of the country. This paper explores perceived health, barriers to access to healthcare, caring practices, food security, and overall financial situation of mothers and young children displaced by the conflict in Ukraine. This is a qualitative study, which collected data through semi-structured in-depth interviews with nine IDP mothers via Skype and Viber with a convenience sample of participants selected through snowball technique. Contrary to the expectations, the perceived physical health of mothers and their children was found not to be affected by conflict and displacement, while psychological distress was often reported. A weak healthcare system, Ukraine’s proneness to informal payments, and heavy bureaucracy to register as an IDP were reported in our study. A precarious social safety net to IDP mothers in Ukraine, poor dietary diversity, and a generalized rupture of vaccine stocks, with halted or delayed vaccinations in children were identified. Increasing social allowances and their timely delivery to IDP mothers might be the most efficient policy measure to improve health and nutrition security. Reestablishment and sustainability of vaccine stocks in Ukraine is urgent to avoid the risks of a public health crisis. Offering psychological support for IDP mothers is recommended. PMID:28075363

Maternal and Child Health of Internally Displaced Persons in Ukraine: A Qualitative Study.

PubMed

Nidzvetska, Svitlana; Rodriguez-Llanes, Jose M; Aujoulat, Isabelle; Gil Cuesta, Julita; Tappis, Hannah; van Loenhout, Joris A F; Guha-Sapir, Debarati

2017-01-09

Due to the conflict that started in spring 2014 in Eastern Ukraine, a total of 1.75 million internally displaced persons (IDPs) fled the area and have been registered in government-controlled areas of the country. This paper explores perceived health, barriers to access to healthcare, caring practices, food security, and overall financial situation of mothers and young children displaced by the conflict in Ukraine. This is a qualitative study, which collected data through semi-structured in-depth interviews with nine IDP mothers via Skype and Viber with a convenience sample of participants selected through snowball technique. Contrary to the expectations, the perceived physical health of mothers and their children was found not to be affected by conflict and displacement, while psychological distress was often reported. A weak healthcare system, Ukraine's proneness to informal payments, and heavy bureaucracy to register as an IDP were reported in our study. A precarious social safety net to IDP mothers in Ukraine, poor dietary diversity, and a generalized rupture of vaccine stocks, with halted or delayed vaccinations in children were identified. Increasing social allowances and their timely delivery to IDP mothers might be the most efficient policy measure to improve health and nutrition security. Reestablishment and sustainability of vaccine stocks in Ukraine is urgent to avoid the risks of a public health crisis. Offering psychological support for IDP mothers is recommended.

Automated Job Controller for Clouds and the Earth's Radiant Energy System (CERES) Production Processing

NASA Astrophysics Data System (ADS)

Gleason, J. L.; Hillyer, T. N.

2011-12-01

Clouds and the Earth's Radiant Energy System (CERES) is one of NASA's highest priority Earth Observing System (EOS) scientific instruments. The CERES science team will integrate data from the CERES Flight Model 5 (FM5) on the NPOESS Preparatory Project (NPP) in addition to the four CERES scanning instrument on Terra and Aqua. The CERES production system consists of over 75 Product Generation Executives (PGEs) maintained by twelve subsystem groups. The processing chain fuses CERES instrument observations with data from 19 other unique sources. The addition of FM5 to over 22 instrument years of data to be reprocessed from flight models 1-4 creates a need for an optimized production processing approach. This poster discusses a new approach, using JBoss and Perl to manage job scheduling and interdependencies between PGEs and external data sources. The new optimized approach uses JBoss to serve handler servlets which regulate PGE-level job interdependencies and job completion notifications. Additional servlets are used to regulate all job submissions from the handlers and to interact with the operator. Perl submission scripts are used to build Process Control Files and to interact directly with the operating system and cluster scheduler. The result is a reduced burden on the operator by algorithmically enforcing a set of rules that determine the optimal time to produce data products with the highest integrity. These rules are designed on a per PGE basis and periodically change. This design provides the means to dynamically update PGE rules at run time and increases the processing throughput by using an event driven controller. The immediate notification of a PGE's completion (an event) allows successor PGEs to launch at the proper time with minimal start up latency, thereby increasing computer system utilization.

Volatiles in interplanetary dust particles: A comparison with CI and CM chondrites

NASA Technical Reports Server (NTRS)

Bustin, Roberta

1992-01-01

In an effort to classify and determine the origin of interplanetary dust particles (IDPs), 14 of these particles were studied using a laser microprobe/mass spectrometer. The mass spectra for these particles varied dramatically. Some particles released hydroxide or water which probably originated in hydroxide-bearing minerals or hydrates. Others produced spectra which included a number of hydrocarbons and resembled meteorite spectra. However, none of the individual IDPs gave spectra which could be matched identically with a particular meteorite type such as a CI or CM carbonaceous chondrite. We believe this was due to the fact that 10-20 micron size IDPs are too small to be representative of the parent body. To verify that the diversity was due primarily to the small particle sizes, small grains of approximately the same size range as the IDPs were obtained from two primitive meteorites, Murchison and Orgueil, and these small meteorite particles were treated exactly like the IDPs. Considerable diversity was observed among individual grains, but a composite spectrum of all the grains from one meteorite closely resembled the spectrum obtained from a much larger sample of that meteorite. A composite spectrum of the 14 IDPs also resembled the spectra of the CM and CI meteorites, pointing to a possible link between IDPs and carbonaceous chondrites. This also illustrates that despite the inherent diversity in particles as small as 10-20 micron, conclusions can be drawn about the possible origin and overall composition of such particles by looking not only at results from individual particles but also by including many particles in a study and basing conclusions on some kind of composite data.

Predicting positive mental health in internally displaced persons in Indonesia: the roles of economic improvement and exposure to violent conflict.

PubMed

Saragih Turnip, Sherly; Sörbom, Dag; Hauff, Edvard

2016-01-01

Positive mental health, rather than just the absence of mental illness, is rarely investigated among the internally displaced persons (IDPs) affected by violent conflict in low-income countries. The purpose of this study was to investigate a model that could explain the interrelationship between factors contributing to positive mental health in displaced populations. In a longitudinal study we examine poverty, exposure to traumatic events and the change of material well-being after one year. We collected data in two consecutive years (2005 and 2006) from a community-based sample of IDPs in Ambon, Indonesia, through face-to-face structured interviews with consenting adults. Participants of this study were IDPs lived in Ambon during the violent conflict period. We interviewed 471 IDPs in the first year and reinterviewed 399 (85%) of the same subjects in the second year. The IDPs possessed good sense of coherence and subjective well-being. Our final model, which was generated by the use of structural equation modeling, fits the data well (χ(2) = 52.51, df = 45, p = .21, CFI = .99, RMSEA = .019). Exposure to violent conflict had a negative impact on IDPs' mental health initially and better economic conditions improved it (r = -.30 and .29 respectively). Mental health status one year previously was a strong predictor of future mental health, followed by individual economic growth in the past year (r = .43 and .29 respectively). On a group level the IDPs were resilient and adaptive to survive in adverse living conditions after devastating violent conflict, and the economic improvement contributed to it.

Results of the Excreta Bioassay Quality Control Program for April 1, 2009 through March 31, 2010

DOE Office of Scientific and Technical Information (OSTI.GOV)

Antonio, Cheryl L.

2012-07-19

A total of 58 urine samples and 10 fecal samples were submitted during the report period (April 1, 2009 through March 31, 2010) to General Engineering Laboratories, South Carolina by the Hanford Internal Dosimetry Program (IDP) to check the accuracy, precision, and detection levels of their analyses. Urine analyses for Sr, 238Pu, 239Pu, 241Am, 243Am 235U, 238U, elemental uranium and fecal analyses for 241Am, 238Pu and 239Pu were tested this year as well as four tissue samples for 238Pu, 239Pu, 241Am and 241Pu. The number of QC urine samples submitted during the report period represented 1.3% of the total samplesmore » submitted. In addition to the samples provided by IDP, GEL was also required to conduct their own QC program, and submit the results of analyses to IDP. About 33% of the analyses processed by GEL during the third year of this contract were quality control samples. GEL tested the performance of 21 radioisotopes, all of which met or exceeded the specifications in the Statement of Work within statistical uncertainty (Table 4).« less

Results of The Excreta Bioassay Quality Control Program For April 1, 2010 Through March 31, 2011

DOE Office of Scientific and Technical Information (OSTI.GOV)

Antonio, Cheryl L.

2012-07-19

A total of 76 urine samples and 10 spiked fecal samples were submitted during the report period (April 1, 2010 through March 31, 2011) to GEL Laboratories, LLC in South Carolina by the Hanford Internal Dosimetry Program (IDP) to check the accuracy, precision, and detection levels of their analyses. Urine analyses for 14C, Sr, for 238Pu, 239Pu, 241Am, 243Am, 235U, 238U, 238U-mass and fecal analyses for 241Am, 238Pu and 239Pu were tested this year. The number of QC urine samples submitted during the report period represented 1.1% of the total samples submitted. In addition to the samples provided by IDP,more » GEL was also required to conduct their own QC program, and submit the results of analyses to IDP. About 31% of the analyses processed by GEL during the first year of contract 112512 were quality control samples. GEL tested the performance of 23 radioisotopes, all of which met or exceeded the specifications in the Statement of Work within statistical uncertainty except the slightly elevated relative bias for 243,244Cm (Table 4).« less

Interplanetary dust particles collected in the stratosphere: observations of atmospheric heating and constraints on their interrelationships and sources.

PubMed

Sandford, S A; Bradley, J P

1989-01-01

The majority of the interplanetary dust particles (IDPs) collected in the stratosphere belong to one of three major classes, the first two dominated by the anhydrous minerals olivine and pyroxene, and the third by hydrous layer-lattice silicates. Infrared spectroscopy and transmission electron microscopy studies show that the different IDP classes represent different types of dust that exist as individual particles in interplanetary space. The majority of the collected IDPs smaller than 30 micrometers in diameter in the layer-lattice silicate and pyroxene classes appear not to have been heated to temperatures above 600 degrees C during atmospheric entry. The relatively low maximum temperatures experienced by these IDPs during atmospheric entry imply that they arrive at the top of the atmosphere with low geocentric encounter velocities. This limits the possible encounter trajectories for these particles to relatively circular, prograde orbits. As a result, it is unlikely that these IDPs are from Earth-crossing comets or asteroids. Asteroids, and comets having low inclinations and perihelia outside 1.2 AU, appear to be the best candidates for the parent bodies of the pyroxene and layer-lattice silicate particles. Chemical and mineralogical information suggests that the pyroxene-rich IDPs are from comets and the layer-lattice silicate-rich IDPs are from asteroids. The collected IDPs dominated by olivine appear to include a larger fraction of particles heating above 600 degrees C, suggesting that these particles were captured from more eccentric orbits. This, and the observation of the infrared spectral features of olivine in several comets suggest these particles have a cometary origin. Since much of the collected dust has apparently been captured from nearly circular, prograde orbits and since there are no appropriate parent bodies presently in such orbits, these results provide an experimental confirmation that the Poynting-Robertson effect exists as a

The spectral properties of interplanetary dust particles

NASA Technical Reports Server (NTRS)

Sandford, Scott A.

1988-01-01

The observed spectral and mineralogical properties of interplanetary dust particles (IDP) allows the conclusion that: (1) the majority of IDP infrared spectra are dominated by olivine, pyroxene, or layer lattice silicate minerals, (2) to the first order the emission spectra of comets Halley and Kohoutek can be matched by mixtures of these IDP infrared types, implying that comets contain mixtures of these different crystalline silicates and may vary from comet to comet and perhaps even within a single comet, (3) do not expect to observe a single 20 micron feature in cometary spectra, (4) carbonaceous materials dominate the visible spectra properties of the IDPs even though the mass in these particles consists primarily of silicates, and (5) the particle characteristics summarized need to be properly accounted for in future cometary emission models.

Delivery of Exogenous Complex Organic Compounds by Solar System Small Bodies and Space Dusts and Its Relevance to Origins of Life

NASA Astrophysics Data System (ADS)

Kobayashi, Kensei; Fushimi, Hidehiko; Motoyama, Takuya; Kaneko, Takeo; Obayashi, Yumiko; Yoshida, Satoshi; Mita, Hajime; Yabuta, Hikaru; Okudaira, Kyoko; Hashimoto, Hirofumi; Yokobori, Shin-Ichi; Yamagishi, Akihiko

A wide variety of organic compounds including amino acid precursors have been detected in such extraterrestrial bodies as carbonaceous chondrites and comets. It was suggested that these organics were formed in quite cold environments. We irradiated frozen mixtures of possible constituents of ice mantles of interstellar dust particles including water, methanol and ammonia with high-energy heavy ions from HIMAC, National Institute of Radiological Science, Japan. Amino acid precursors with complex structures were detected whose molecular weights are up to a few thousands. Such complex amino acid precursors are much stronger than free amino acids against radiation. Such organics could have been incorporated in solar system small bodies after the formation of the solar system and delivered to the primitive Earth. Possible carriers of such organics are meteorites, comets and interplanetary dust particles (IDPs) that were formed from comets and meteorites. It is suggested that IDPs brought much more organics than meteorites and comets. However, nature of organics in IDPs is little known, since they have been collected only in terrestrial biosphere. We are planning a space experiments named Tanpopo, where IDPs would be collected in aerogel equipped on the Exposure Facility of the International Space Station. In addition, amino acids and their relating compounds would be exposed to space environments to see their possible alteration processes in the interplanetary space. We will report some preliminary results for the preparation of the mission including the capture of amino acid-containing particles at high velocity with ultra-low density aerogel.

Native Hydrophobic Binding Interactions at the Transition State for Association between the TAZ1 Domain of CBP and the Disordered TAD-STAT2 Are Not a Requirement.

PubMed

Lindström, Ida; Dogan, Jakob

2017-08-15

A significant fraction of the eukaryotic proteome consists of proteins that are either partially or completely disordered under native-like conditions. Intrinsically disordered proteins (IDPs) are common in protein-protein interactions and are involved in numerous cellular processes. Although many proteins have been identified as disordered, much less is known about the binding mechanisms of the coupled binding and folding reactions involving IDPs. Here we have analyzed the rate-limiting transition state for binding between the TAZ1 domain of CREB binding protein and the intrinsically disordered transactivation domain of STAT2 (TAD-STAT2) by site-directed mutagenesis and kinetic experiments (Φ-value analysis) and found that the native protein-protein binding interface is not formed at the transition state for binding. Instead, native hydrophobic binding interactions form late, after the rate-limiting barrier has been crossed. The association rate constant in the absence of electrostatic enhancement was determined to be rather high. This is consistent with the Φ-value analysis, which showed that there are few or no obligatory native contacts. Also, linear free energy relationships clearly demonstrate that native interactions are cooperatively formed, a scenario that has usually been observed for proteins that fold according to the so-called nucleation-condensation mechanism. Thus, native hydrophobic binding interactions at the rate-limiting transition state for association between TAD-STAT2 and TAZ1 are not a requirement, which is generally in agreement with previous findings on other IDP systems and might be a common mechanism for IDPs.

Automated calibration of the Suomi National Polar-Orbiting Partnership (S-NPP) Visible Infrared Imaging Radiometer Suite (VIIRS) reflective solar bands

NASA Astrophysics Data System (ADS)

Rausch, Kameron; Houchin, Scott; Cardema, Jason; Moy, Gabriel; Haas, Evan; De Luccia, Frank J.

2013-12-01

National Polar-Orbiting Partnership (S-NPP) Visible Infrared Imaging Radiometer Suite (VIIRS) reflective bands are currently calibrated via weekly updates to look-up tables (LUTs) utilized by operational ground processing in the Joint Polar Satellite System Interface Data Processing Segment (IDPS). The parameters in these LUTs must be predicted ahead 2 weeks and cannot adequately track the dynamically varying response characteristics of the instrument. As a result, spurious "predict-ahead" calibration errors of the order of 0.1% or greater are routinely introduced into the calibrated reflectances and radiances produced by IDPS in sensor data records (SDRs). Spurious calibration errors of this magnitude adversely impact the quality of downstream environmental data records (EDRs) derived from VIIRS SDRs such as Ocean Color/Chlorophyll and cause increased striping and band-to-band radiometric calibration uncertainty of SDR products. A novel algorithm that fully automates reflective band calibration has been developed for implementation in IDPS in late 2013. Automating the reflective solar band (RSB) calibration is extremely challenging and represents a significant advancement over the manner in which RSB calibration has traditionally been performed in heritage instruments such as the Moderate Resolution Imaging Spectroradiometer. The automated algorithm applies calibration data almost immediately after their acquisition by the instrument from views of space and on-onboard calibration sources, thereby eliminating the predict-ahead errors associated with the current offline calibration process. This new algorithm, when implemented, will significantly improve the quality of VIIRS reflective band SDRs and consequently the quality of EDRs produced from these SDRs.

Summer 2006 war on Lebanon: a lesson in community resilience.

PubMed

Nuwayhid, Iman; Zurayk, Huda; Yamout, Rouham; Cortas, Chadi S

2011-01-01

The internally displaced persons (IDPs) during the July 2006 war in Lebanon exhibited a high level of community resilience, affirmed by relief agencies and public health professionals. Data from personal observations, interviews, meetings and published material were used to examine factors contributing to this resilience. Findings suggested that community resilience is a process rather than an outcome. The sense of a collective identity, prior experience with wars and social support networks have contributed to building up IDP's resilience over time, while community cohesiveness, adequate public health interventions, social solidarity and a connected political leadership helped to sustain it during and shortly after the war. This paper examines implications for public health professionals and argues for a paradigm shift in disaster relief practice.

Discovery of nuclear tracks in interplanetary dust

NASA Technical Reports Server (NTRS)

Bradley, J. P.; Brownlee, D. E.; Fraundorf, P.

1984-01-01

Nuclear tracks have been identified in interplanetary dust particles (IDP's) collected from the stratosphere. The presence of tracks unambiguously confirms the extraterrestrial nature of IDP's, and the high track densities (10 to the 10th to 10 to the 11th per square centimeter) suggest an exposure age of approximately 10,000 years within the inner solar system. Tracks also provide an upper temperature limit for the heating of IDP's during atmospheric entry, thereby making it possible to distinguish between pristine and thermally modified micrometeorites.

What if chondritic porous interplanetary dust particles are not the real McCoy

NASA Astrophysics Data System (ADS)

Rietmeijer, Frans J. M.

To select a target comet for a Comet Nucleus Sample Return Mission (CNSRM) it is necessary to have an experimental data base to evaluate the extent of diversity and similarity of comets. For example, the physical properties (e.g., low density) of chondritic porous (CP) interplanetary dust particles (IDPs) are believed to resemble these properties of cometary dust although it is yet to be demonstrated that the porous structure of CP IDPs is inherent to presolar dust particles stored in comet nuclei. Porous structures of IDPs could conceivably form during sublimation at the surface of active comet nuclei. Porous structures are also obtained during annealing of amorphous Mg-SiO smokes which initially forms porous aggregates of olivine + platey tridymite and which, upon continued annealing, react to fluffy enstatite aggregates. It is therefore uncertain that CP IDPs are entirely composed of unmetamorphosed presolar dust. Conceivably, new minerals and textures may form in situ in nuclei of active comets as a function of their individual thermal history. Unmetamorphosed comet dust is probably structurally amorphous. Thermal annealing of this dust can produce ultra fine-grained minerals and this ultrafine grain size of CP IDPs should be considered in assessments of aqueous alterations that could affect presolar dust in comet nuclei between 200 and 400 K. Devitrification and hydration may occur in situ in ice-dust mixtures and the mantle of active comet nuclei. Devitrification, or uncontrolled crystallization, of amorphous precursor dust can produce a range of chemical compositions of ultrafine-grained minerals and (non-equilibrium) mineral assemblages and textures in dust contained in comet nuclei as a function of period and trajectory of orbit and number of perihelion passages (not considering internal heating). Thus, experimental data on relevant processes and reaction rates between 200 and 400 K are needed in order to evaluate comet selection, penetration depth for

What if chondritic porous interplanetary dust particles are not the real McCoy

NASA Technical Reports Server (NTRS)

Rietmeijer, Frans J. M.

1989-01-01

To select a target comet for a Comet Nucleus Sample Return Mission (CNSRM) it is necessary to have an experimental data base to evaluate the extent of diversity and similarity of comets. For example, the physical properties (e.g., low density) of chondritic porous (CP) interplanetary dust particles (IDPs) are believed to resemble these properties of cometary dust although it is yet to be demonstrated that the porous structure of CP IDPs is inherent to presolar dust particles stored in comet nuclei. Porous structures of IDPs could conceivably form during sublimation at the surface of active comet nuclei. Porous structures are also obtained during annealing of amorphous Mg-SiO smokes which initially forms porous aggregates of olivine + platey tridymite and which, upon continued annealing, react to fluffy enstatite aggregates. It is therefore uncertain that CP IDPs are entirely composed of unmetamorphosed presolar dust. Conceivably, new minerals and textures may form in situ in nuclei of active comets as a function of their individual thermal history. Unmetamorphosed comet dust is probably structurally amorphous. Thermal annealing of this dust can produce ultra fine-grained minerals and this ultrafine grain size of CP IDPs should be considered in assessments of aqueous alterations that could affect presolar dust in comet nuclei between 200 and 400 K. Devitrification and hydration may occur in situ in ice-dust mixtures and the mantle of active comet nuclei. Devitrification, or uncontrolled crystallization, of amorphous precursor dust can produce a range of chemical compositions of ultrafine-grained minerals and (non-equilibrium) mineral assemblages and textures in dust contained in comet nuclei as a function of period and trajectory of orbit and number of perihelion passages (not considering internal heating). Thus, experimental data on relevant processes and reaction rates between 200 and 400 K are needed in order to evaluate comet selection, penetration depth for

Water and organics in interplanetary dust particles

NASA Astrophysics Data System (ADS)

Bradley, John P.

2015-08-01

Interplanetary dust particles (IDPs) and larger micrometeorites (MMs) impinge on the upper atmosphere where they decelerate at ~90 km altitude and settle to the Earth’s surface. Comets and asteroids are the major sources and the flux, 30,000-40,000 tons/yr, is comparable to the mass of larger meteorites impacting the Earth’s surface. The sedimentary record suggests that the flux was much higher on the early Earth. The chondritic porous (CP) subset of IDPs together with their larger counterparts, ultracarbonaceous micrometeorites (UCMMs), appear to be unique among known meteoritic materials in that they are composed almost exclusively of anhydrous minerals, some of them contain >> 50% organic carbon by volume as well as the highest abundances of presolar silicate grains including GEMS. D/H and 15N abundances implicate the Oort Cloud or presolar molecular cloud as likely sources of the organic carbon. Prior to atmospheric entry, IDPs and MMs spend ~104-105 year lifetimes in solar orbit where their surfaces develop amorphous space weathered rims from exposure to the solar wind (SW). Similar rims are observed on lunar soil grains and on asteroid Itokawa regolith grains. Using valence electron energy-loss spectroscopy (VEELS) we have detected radiolytic water in the rims on IDPs formed by the interaction of solar wind protons with oxygen in silicate minerals. Therefore, IDPs and MMs continuously deliver both water and organics to the earth and other terrestrial planets. The interaction of protons with oxygen-rich minerals to form water is a universal process.Affiliations:a University of Hawaii at Manoa, Hawaii Institute of Geophysics and Planetology, 1680 East-West Road, Honolulu, HI 96822, USA.b National Center for Electron Microscopy, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA.c Materials Science Division, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA.d Department of Materials Science & Engineering, University of California

Intrinsically Disordered Proteins and the Origins of Multicellular Organisms

NASA Astrophysics Data System (ADS)

Dunker, A. Keith

In simple multicellular organisms all of the cells are in direct contact with the surrounding milieu, whereas in complex multicellular organisms some cells are completely surrounded by other cells. Current phylogenetic trees indicate that complex multicellular organisms evolved independently from unicellular ancestors about 10 times, and only among the eukaryotes, including once for animals, twice each for green, red, and brown algae, and thrice for fungi. Given these multiple independent evolutionary lineages, we asked two questions: 1. Which molecular functions underpinned the evolution of multicellular organisms?; and, 2. Which of these molecular functions depend on intrinsically disordered proteins (IDPs)? Compared to unicellularity, multicellularity requires the advent of molecules for cellular adhesion, for cell-cell communication and for developmental programs. In addition, the developmental programs need to be regulated over space and time. Finally, each multicellular organism has cell-specific biochemistry and physiology. Thus, the evolution of complex multicellular organisms from unicellular ancestors required five new classes of functions. To answer the second question we used Key-words in Swiss Protein ranked for associations with predictions of protein structure or disorder. With a Z-score of 18.8 compared to random-function proteins, à differentiation was the biological process most strongly associated with IDPs. As expected from this result, large numbers of individual proteins associated with differentiation exhibit substantial regions of predicted disorder. For the animals for which there is the most readily available data all five of the underpinning molecular functions for multicellularity were found to depend critically on IDP-based mechanisms and other evidence supports these ideas. While the data are more sparse, IDPs seem to similarly underlie the five new classes of functions for plants and fungi as well, suggesting that IDPs were indeed

Evaluation of Operational Albedo Algorithms For AVHRR, MODIS and VIIRS: Case Studies in Southern Africa

NASA Astrophysics Data System (ADS)

Privette, J. L.; Schaaf, C. B.; Saleous, N.; Liang, S.

2004-12-01

Shortwave broadband albedo is the fundamental surface variable that partitions solar irradiance into energy available to the land biophysical system and energy reflected back into the atmosphere. Albedo varies with land cover, vegetation phenological stage, surface wetness, solar angle, and atmospheric condition, among other variables. For these reasons, a consistent and normalized albedo time series is needed to accurately model weather, climate and ecological trends. Although an empirically-derived coarse-scale albedo from the 20-year NOAA AVHRR record (Sellers et al., 1996) is available, an operational moderate resolution global product first became available from NASA's MODIS sensor. The validated MODIS product now provides the benchmark upon which to compare albedo generated through 1) reprocessing of the historic AVHRR record and 2) operational processing of data from the future National Polar-Orbiting Environmental Satellite System's (NPOESS) Visible/Infrared Imager Radiometer Suite (VIIRS). Unfortunately, different instrument characteristics (e.g., spectral bands, spatial resolution), processing approaches (e.g., latency requirements, ancillary data availability) and even product definitions (black sky albedo, white sky albedo, actual or blue sky albedo) complicate the development of the desired multi-mission (AVHRR to MODIS to VIIRS) albedo time series -- a so-called Climate Data Record. This presentation will describe the different albedo algorithms used with AVHRR, MODIS and VIIRS, and compare their results against field measurements collected over two semi-arid sites in southern Africa. We also describe the MODIS-derived VIIRS proxy data we developed to predict NPOESS albedo characteristics. We conclude with a strategy to develop a seamless Climate Data Record from 1982- to 2020.

Results of Automated Retinal Image Analysis for Detection of Diabetic Retinopathy from the Nakuru Study, Kenya.

PubMed

Hansen, Morten B; Abràmoff, Michael D; Folk, James C; Mathenge, Wanjiku; Bastawrous, Andrew; Peto, Tunde

2015-01-01

Digital retinal imaging is an established method of screening for diabetic retinopathy (DR). It has been established that currently about 1% of the world's blind or visually impaired is due to DR. However, the increasing prevalence of diabetes mellitus and DR is creating an increased workload on those with expertise in grading retinal images. Safe and reliable automated analysis of retinal images may support screening services worldwide. This study aimed to compare the Iowa Detection Program (IDP) ability to detect diabetic eye diseases (DED) to human grading carried out at Moorfields Reading Centre on the population of Nakuru Study from Kenya. Retinal images were taken from participants of the Nakuru Eye Disease Study in Kenya in 2007/08 (n = 4,381 participants [NW6 Topcon Digital Retinal Camera]). First, human grading was performed for the presence or absence of DR, and for those with DR this was sub-divided in to referable or non-referable DR. The automated IDP software was deployed to identify those with DR and also to categorize the severity of DR. The primary outcomes were sensitivity, specificity, and positive and negative predictive value of IDP versus the human grader as reference standard. Altogether 3,460 participants were included. 113 had DED, giving a prevalence of 3.3% (95% CI, 2.7-3.9%). Sensitivity of the IDP to detect DED as by the human grading was 91.0% (95% CI, 88.0-93.4%). The IDP ability to detect DED gave an AUC of 0.878 (95% CI 0.850-0.905). It showed a negative predictive value of 98%. The IDP missed no vision threatening retinopathy in any patients and none of the false negative cases met criteria for treatment. In this epidemiological sample, the IDP's grading was comparable to that of human graders'. It therefore might be feasible to consider inclusion into usual epidemiological grading.

Biomechanical analysis of disc pressure and facet contact force after simulated two-level cervical surgeries (fusion and arthroplasty) and hybrid surgery.

PubMed

Park, Jon; Shin, Jun Jae; Lim, Jesse

2014-12-01

The objective of this study was designed to compare 2-level cervical disc surgery (2-level anterior cervical discectomy and fusion [ACDF] or disc arthroplasty) and hybrid surgery (ACDF/arthroplasty) in terms of postoperative adjacent-level intradiscal pressure (IDP) and facet contact force (FCF). Twenty-four cadaveric cervical spines (C3-T2) were tested in various modes, including extension, flexion, and bilateral axial rotation, to compare adjacent-level IDP and FCF after specified treatments as follows: 1) C5-C6 arthroplasty using ProDisc-C (Synthes Spine, West Chester, Pennsylvania, USA) and C6-C7 ACDF, 2) C5-C6 ACDF and C6-C7 arthroplasty using ProDisc-C, 3) 2-level C5-C6/C6-C7 disc arthroplasties, and 4) 2-level C5-C6/C6-C7 ACDF. IDPs were recorded at anterior, central, and posterior disc portions. After 2-level cervical arthrodesis (ACDF), IDP increased significantly at the anterior annulus of distal adjacent-level disc during flexion and axial rotation and at the center of proximal adjacent-level disc during flexion. In contrast, after cervical specified treatments, including disc arthroplasty (2-level disc arthroplasties and hybrid surgery), IDP decreased significantly at the anterior annulus of distal adjacent-level disc during flexion and extension and was unchanged at the center of proximal adjacent-level disc during flexion. Two-level cervical arthrodesis also tended to adversely impact facet loads, increasing distal rather than proximal adjacent-level FCF. Both hybrid surgery and 2-level arthroplasties seem to offer significant advantages over 2-level arthrodesis by reducing IDP at adjacent levels and approximating FCF of an intact spine. These findings suggest that cervical arthroplasties and hybrid surgery are an alternative to reduce IDP and facet loads at adjacent levels. Copyright © 2014 Elsevier Inc. All rights reserved.

Principal Components Constrain Dynamic Pyrometamorphism in a Partially Melted Interplanetary Dust Particle

NASA Astrophysics Data System (ADS)

Rietmeijer, F. J. M.

1996-03-01

All interplanetary dust particles [IDPs] that decelerate in the Earth's atmosphere experience flash heating whereby their orbital velocity (km s^-1) is reduced to cm s^-1. Iron-oxide rims that may either be continuous on the particle surface, or discontinuous along its perimeter, occur on many IDPs. It is the most conspicuous mineralogical indicator of dynamic pyrometamorphism and its formation is interpreted as 'intense' IDP heating. It appears that the formation of these magnetite and maghemite rims is intimately linked to the presence of Fe,Ni-sulfides in the pre-entry particle. In this regard chondritic porous (CP) IDP L2011K7 may be an unusual particle. This porous aggregate consists of S-free polyphase units (PUs), nonstoichiometric diopside and Mg-rich wollastonite single-crystals and small, accessory Fe,Ni-sulfide grains. Only a few Fe-oxide nanograins are present along its perimeter. The nonstoichiometry of its Ca,Mg-clinopyroxenes indicates incongruent melting or vaporization that was experimentally determined at 1300 degrees-1400 degrees C. The CP IDP L2011K7 is probably of cometary origin since active comet nuclei are the most likely sources for uncompacted aggregate IDPs whereby their low density (< 1 g cm^-3) enhances atmospheric entry survival of large particles. In addition, the fluffy texture which inhibits chemical exchange among its components further enhances their survival unless compaction occurs during deceleration in the Earth's atmosphere.

MFIB: a repository of protein complexes with mutual folding induced by binding.

PubMed

Fichó, Erzsébet; Reményi, István; Simon, István; Mészáros, Bálint

2017-11-15

It is commonplace that intrinsically disordered proteins (IDPs) are involved in crucial interactions in the living cell. However, the study of protein complexes formed exclusively by IDPs is hindered by the lack of data and such analyses remain sporadic. Systematic studies benefited other types of protein-protein interactions paving a way from basic science to therapeutics; yet these efforts require reliable datasets that are currently lacking for synergistically folding complexes of IDPs. Here we present the Mutual Folding Induced by Binding (MFIB) database, the first systematic collection of complexes formed exclusively by IDPs. MFIB contains an order of magnitude more data than any dataset used in corresponding studies and offers a wide coverage of known IDP complexes in terms of flexibility, oligomeric composition and protein function from all domains of life. The included complexes are grouped using a hierarchical classification and are complemented with structural and functional annotations. MFIB is backed by a firm development team and infrastructure, and together with possible future community collaboration it will provide the cornerstone for structural and functional studies of IDP complexes. MFIB is freely accessible at http://mfib.enzim.ttk.mta.hu/. The MFIB application is hosted by Apache web server and was implemented in PHP. To enrich querying features and to enhance backend performance a MySQL database was also created. simon.istvan@ttk.mta.hu, meszaros.balint@ttk.mta.hu. Supplementary data are available at Bioinformatics online. © The Author 2017. Published by Oxford University Press.

Structural and Kinetic Characterization of the Intrinsically Disordered Protein SeV NTAIL through Enhanced Sampling Simulations.

PubMed

Bernetti, Mattia; Masetti, Matteo; Pietrucci, Fabio; Blackledge, Martin; Jensen, Malene Ringkjobing; Recanatini, Maurizio; Mollica, Luca; Cavalli, Andrea

2017-10-19

Intrinsically disordered proteins (IDPs) are emerging as an important class of the proteome. Being able to interact with different molecular targets, they participate in many physiological and pathological activities. However, due to their intrinsically heterogeneous nature, determining the equilibrium properties of IDPs is still a challenge for biophysics. Herein, we applied state-of-the-art enhanced sampling methods to Sev N TAIL , a test case of IDPs, and constructed a bin-based kinetic model to unveil the underlying kinetics. To validate our simulation strategy, we compared the predicted NMR properties against available experimental data. Our simulations reveal a rough free-energy surface comprising multiple local minima, which are separated by low energy barriers. Moreover, we identified interconversion rates between the main kinetic states, which lie in the sub-μs time scales, as suggested in previous works for Sev N TAIL . Therefore, the emerging picture is in agreement with the atomic-level properties possessed by the free IDP in solution. By providing both a thermodynamic and kinetic characterization of this IDP test case, our study demonstrates how computational methods can be effective tools for studying this challenging class of proteins.

The Syrian public health and humanitarian crisis: A 'displacement' in global governance?

PubMed

Akbarzada, Sumaira; Mackey, Tim K

2018-07-01

Ongoing failure by the international community to resolve the Syrian conflict has led to destruction of critical infrastructure. This includes the collapse of the Syrian health system, leaving millions of internally displaced persons (IDPs) in urgent need of healthcare services. As the conflict intensifies, IDP populations are suffering from infectious and non-communicable disease risks, poor maternal and child health outcomes, trauma, and mental health issues, while healthcare workers continually exit the country. Healthcare workers who remain face significant challenges, including systematic attacks on healthcare facilities and conditions that severely inhibit healthcare delivery and assistance. Within this conflict-driven public health crisis, the most susceptible population is arguably the IDP. Though the fundamental 'right to health' is a recognised international legal principle, its application is inadequate due to limited recognition by the UN Security Council and stymied global governance by the broader international community. These factors have also negatively impacted other vulnerable groups other than IDPs, such as refugees and ethnic minorities, who may or may not be displaced. Hence, this article reviews the current Syrian conflict, assesses challenges with local and global governance for IDPs, and explores potential governance solutions needed to address this health and humanitarian crisis.

New Manganese Silicide Mineral Phase in an Interplanetary Dust Particle

NASA Technical Reports Server (NTRS)

Nakamura-Messenger, K.; Keller, L. P.; Clemett, S. J.; Jones, J. H.; Palma, R. L.; Pepin, R. O.; Kloeck, W.; Zolensky, M. E.; Messenger, S.

2008-01-01

Comet 26P/Grigg-Skjellerup was identified as a source of an Earth-crossing dust stream with low Earth-encounter velocities, with peak anticipated fluxes during April in 2003 and 2004 [1]. In response to this prediction, NASA performed dedicated stratospheric dust collections using high altitude aircraft to target potential interplanetary dust particles (IDPs) from this comet stream in April 2003. Several IDPs from this collection have shown unusually low noble gas abundances [2] consistent with the predicted short space exposure ages of Grigg-Skjellerup dust particles [1]. High abundances of large D enrichments [3] and presolar grains [4] in IDPs from this collection are also consistent with an origin from the comet Grigg-Skjellerup. Here we report a new mineral from one of the cluster IDPs of the "Grigg-Skjellerup" collection, L2055. Our report focuses on an unusual manganese-iron-chromium silicide phase that, to our knowledge, has not been observed previously in nature. This unique phase may also shed light on the genesis of the enigmatic low-Fe,Mn-enriched (LIME) olivine that has been previously reported in IDPs and meteorites [5].

Diagnostic value of the near-nerve needle sensory nerve conduction in sensory inflammatory demyelinating polyneuropathy.

PubMed

Odabasi, Zeki; Oh, Shin J

2018-03-01

In this study we report the diagnostic value of the near-nerve needle sensory nerve conduction study (NNN-SNCS) in sensory inflammatory demyelinating polyneuropathy (IDP) in which the routine nerve conduction study was normal or non-diagnostic. The NNN-SNCS was performed to identify demyelination in the plantar nerves in 14 patients and in the median or ulnar nerve in 2 patients with sensory IDP. In 16 patients with sensory IDP, routine NCSs were either normal or non-diagnostic for demyelination. Demyelination was identified by NNN-SNCS by dispersion and/or slow nerve conduction velocity (NCV) below the demyelination marker. Immunotherapy was initiated in 11 patients, 10 of whom improved or remained stable. NNN-SNCS played an essential role in identifying demyelinaton in 16 patients with sensory IDP, leading to proper treatment. Muscle Nerve 57: 414-418, 2018. © 2017 Wiley Periodicals, Inc.

Analysis of the Organic Matter in Interplanetary Dust Particles: Clues to the Organic Matter in Comets, Asteroids, and Interstellar Grains

NASA Technical Reports Server (NTRS)

Flynn, G. J.; Keller, L. P.

2003-01-01

Reflection spectroscopy suggests the C- , P-, and D-types of asteroids contain abundant carbon, but these Vis-nearIR spectra are featureless, providing no information on the type(s) of carbonaceous matter. Infrared spectroscopy demonstrates that organic carbon is a significant component in comets and as grains or grain coatings in the interstellar medium. Most of the interplanetary dust particles (IDPs) recovered from the Earth s stratosphere are believed to be fragments from asteroids or comets, thus characterization of the carbon in IDPs provides the opportunity to determine the type(s) and abundance of organic matter in asteroids and comets. Some IDPs exhibit isotopic excesses of D and N-15, indicating the presence of interstellar material. The characterization of the carbon in these IDPs, and particularly any carbon spatially associated with the isotopic anomalies, provides the opportunity to characterize interstellar organic matter.

Meta-structure correlation in protein space unveils different selection rules for folded and intrinsically disordered proteins.

PubMed

Naranjo, Yandi; Pons, Miquel; Konrat, Robert

2012-01-01

The number of existing protein sequences spans a very small fraction of sequence space. Natural proteins have overcome a strong negative selective pressure to avoid the formation of insoluble aggregates. Stably folded globular proteins and intrinsically disordered proteins (IDPs) use alternative solutions to the aggregation problem. While in globular proteins folding minimizes the access to aggregation prone regions, IDPs on average display large exposed contact areas. Here, we introduce the concept of average meta-structure correlation maps to analyze sequence space. Using this novel conceptual view we show that representative ensembles of folded and ID proteins show distinct characteristics and respond differently to sequence randomization. By studying the way evolutionary constraints act on IDPs to disable a negative function (aggregation) we might gain insight into the mechanisms by which function-enabling information is encoded in IDPs.

Discrete Molecular Dynamics Can Predict Helical Prestructured Motifs in Disordered Proteins

PubMed Central

Han, Kyou-Hoon; Dokholyan, Nikolay V.; Tompa, Péter; Kalmár, Lajos; Hegedűs, Tamás

2014-01-01

Intrinsically disordered proteins (IDPs) lack a stable tertiary structure, but their short binding regions termed Pre-Structured Motifs (PreSMo) can form transient secondary structure elements in solution. Although disordered proteins are crucial in many biological processes and designing strategies to modulate their function is highly important, both experimental and computational tools to describe their conformational ensembles and the initial steps of folding are sparse. Here we report that discrete molecular dynamics (DMD) simulations combined with replica exchange (RX) method efficiently samples the conformational space and detects regions populating α-helical conformational states in disordered protein regions. While the available computational methods predict secondary structural propensities in IDPs based on the observation of protein-protein interactions, our ab initio method rests on physical principles of protein folding and dynamics. We show that RX-DMD predicts α-PreSMos with high confidence confirmed by comparison to experimental NMR data. Moreover, the method also can dissect α-PreSMos in close vicinity to each other and indicate helix stability. Importantly, simulations with disordered regions forming helices in X-ray structures of complexes indicate that a preformed helix is frequently the binding element itself, while in other cases it may have a role in initiating the binding process. Our results indicate that RX-DMD provides a breakthrough in the structural and dynamical characterization of disordered proteins by generating the structural ensembles of IDPs even when experimental data are not available. PMID:24763499

NPP Clouds and the Earth's Radiant Energy System (CERES) Predicted Sensor Performance Calibration and Preliminary Data Product Performance

NASA Technical Reports Server (NTRS)

Priestly, Kory; Smith, George L.; Thomas, Susan; Maddock, Suzanne L.

2009-01-01

Continuation of the Earth Radiation Budget (ERB) Climate Data Record (CDR) has been identified as critical in the 2007 NRC Decadal Survey, the Global Climate Observing System WCRP report, and in an assessment titled Impacts of NPOESS Nunn-McCurdy Certification on Joint NASA-NOAA Climate Goals. In response, NASA, NOAA and NPOESS agreed in early 2008 to fly the final existing CERES Flight Model (FM-5) on the NPP spacecraft for launch in 2010. Future opportunities for ERB CDR continuity consist of procuring an additional CERES Sensor with modest performance upgrades for flight on the NPOESS C1 spacecraft in 2013, followed by a new CERES follow-on sensor for flight in 2018 on the NPOESS C3 spacecraft. While science goals remain unchanged for the long-term ERB Climate Data Record, it is now understood that the task of achieving these goals is more difficult for two reasons. The first is an increased understanding of the dynamics of the Earth/atmosphere system which demonstrates that rigorous separation of natural variability from anthropogenic change on decadal time scales requires higher accuracy and stability than originally envisioned. Secondly, future implementation scenarios involve less redundancy in flight hardware (1 vs. 2 orbits and operational sensors) resulting in higher risk of loss of continuity and reduced number of independent observations to characterize performance of individual sensors. Although EOS CERES CDR's realize a factor of 2 to 4 improvement in accuracy and stability over previous ERBE CDR's, future sensors will require an additional factor of 2 improvement to answer rigorously the science questions moving forward. Modest investments, defined through the CERES Science Team s 30-year operational history of the EOS CERES sensors, in onboard calibration hardware and pre-flight calibration and test program will ensure meeting these goals while reducing costs in re-processing scientific datasets. The CERES FM-5 pre-flight radiometric

Cell cycle regulation by the intrinsically disordered proteins p21 and p27.

PubMed

Yoon, Mi-Kyung; Mitrea, Diana M; Ou, Li; Kriwacki, Richard W

2012-10-01

Today, it is widely accepted that proteins that lack highly defined globular three-dimensional structures, termed IDPs (intrinsically disordered proteins), play key roles in myriad biological processes. Our understanding of how intrinsic disorder mediates biological function is, however, incomplete. In the present paper, we review disorder-mediated cell cycle regulation by two intrinsically disordered proteins, p21 and p27. A structural adaptation mechanism involving a stretchable dynamic linker helix allows p21 to promiscuously recognize the various Cdk (cyclin-dependent kinase)-cyclin complexes that regulate cell division. Disorder within p27 mediates transmission of an N-terminal tyrosine phosphorylation signal to a C-terminal threonine phosphorylation, constituting a signalling conduit. These mechanisms are mediated by folding upon binding p21/p27's regulatory targets. However, residual disorder within the bound state contributes critically to these functional mechanisms. Our studies provide insights into how intrinsic protein disorder mediates regulatory processes and opportunities for designing drugs that target cancer-associated IDPs.

Lessons Learned From Developing Three Generations of Remote Sensing Science Data Processing Systems

NASA Technical Reports Server (NTRS)

Tilmes, Curt; Fleig, Albert J.

2005-01-01

The Biospheric Information Systems Branch at NASA s Goddard Space Flight Center has developed three generations of Science Investigator-led Processing Systems for use with various remote sensing instruments. The first system is used for data from the MODIS instruments flown on NASA s Earth Observing Systems @OS) Terra and Aqua Spacecraft launched in 1999 and 2002 respectively. The second generation is for the Ozone Measuring Instrument flying on the EOS Aura spacecraft launched in 2004. We are now developing a third generation of the system for evaluation science data processing for the Ozone Mapping and Profiler Suite (OMPS) to be flown by the NPOESS Preparatory Project (NPP) in 2006. The initial system was based on large scale proprietary hardware, operating and database systems. The current OMI system and the OMPS system being developed are based on commodity hardware, the LINUX Operating System and on PostgreSQL, an Open Source RDBMS. The new system distributes its data archive across multiple server hosts and processes jobs on multiple processor boxes. We have created several instances of this system, including one for operational processing, one for testing and reprocessing and one for applications development and scientific analysis. Prior to receiving the first data from OMI we applied the system to reprocessing information from the Solar Backscatter Ultraviolet (SBUV) and Total Ozone Mapping Spectrometer (TOMS) instruments flown from 1978 until now. The system was able to process 25 years (108,000 orbits) of data and produce 800,000 files (400 GiB) of level 2 and level 3 products in less than a week. We will describe the lessons we have learned and tradeoffs between system design, hardware, operating systems, operational staffing, user support and operational procedures. During each generational phase, the system has become more generic and reusable. While the system is not currently shrink wrapped we believe it is to the point where it could be readily

The alphabet of intrinsic disorder

PubMed Central

Theillet, Francois-Xavier; Kalmar, Lajos; Tompa, Peter; Han, Kyou-Hoon; Selenko, Philipp; Dunker, A. Keith; Daughdrill, Gary W.; Uversky, Vladimir N

2013-01-01

A significant fraction of every proteome is occupied by biologically active proteins that do not form unique three-dimensional structures. These intrinsically disordered proteins (IDPs) and IDP regions (IDPRs) have essential biological functions and are characterized by extensive structural plasticity. Such structural and functional behavior is encoded in the amino acid sequences of IDPs/IDPRs, which are enriched in disorder-promoting residues and depleted in order-promoting residues. In fact, amino acid residues can be arranged according to their disorder-promoting tendency to form an alphabet of intrinsic disorder that defines the structural complexity and diversity of IDPs/IDPRs. This review is the first in a series of publications dedicated to the roles that different amino acid residues play in defining the phenomenon of protein intrinsic disorder. We start with proline because data suggests that of the 20 common amino acid residues, this one is the most disorder-promoting. PMID:28516008

DOE Office of Scientific and Technical Information (OSTI.GOV)

Riback, Joshua A.; Bowman, Micayla A.; Zmyslowski, Adam M.

A substantial fraction of the proteome is intrinsically disordered, and even well-folded proteins adopt non-native geometries during synthesis, folding, transport, and turnover. Characterization of intrinsically disordered proteins (IDPs) is challenging, in part because of a lack of accurate physical models and the difficulty of interpreting experimental results. We have developed a general method to extract the dimensions and solvent quality (self-interactions) of IDPs from a single small-angle x-ray scattering measurement. We applied this procedure to a variety of IDPs and found that even IDPs with low net charge and high hydrophobicity remain highly expanded in water, contrary to the generalmore » expectation that protein-like sequences collapse in water. Our results suggest that the unfolded state of most foldable sequences is expanded; we conjecture that this property was selected by evolution to minimize misfolding and aggregation.« less

Biophysical characterization of α-synuclein and its controversial structure

PubMed Central

Alderson, T Reid; Markley, John L

2013-01-01

α-synuclein, a presynaptic protein of poorly defined function, constitutes the main component of Parkinson disease-associated Lewy bodies. Extensive biophysical investigations have provided evidence that isolated α-synuclein is an intrinsically disordered protein (IDP) in vitro. Subsequently serving as a model IDP in numerous studies, α-synuclein has aided in the development of many technologies used to characterize IDPs and arguably represents the most thoroughly analyzed IDP to date. Recent reports, however, have challenged the disordered nature of α-synuclein inside cells and have instead proposed a physiologically relevant helical tetramer. Despite α-synuclein’s rich biophysical history, a single coherent picture has not yet emerged concerning its in vivo structure, dynamics, and physiological role(s). We present herein a review of the biophysical discoveries, developments, and models pertinent to the characterization of α-synuclein’s structure and analysis of the native tetramer controversy. PMID:24634806

Photographic photometry with Iris diaphragm photometers

NASA Technical Reports Server (NTRS)

Schaefer, B. E.

1981-01-01

A general method is presented for solving problems encountered in the analysis of Iris diaphragm photometer (IDP) data. The method is used to derive the general shape of the calibration curve, allowing both a more accurate fit to the IDP data for comparison stars and extrapolation to magnitude ranges for which no comparison stars are measured. The profile of starlight incident and the characteristic curve of the plate are both assumed and then used to derive the profile of the star image. An IDP reading is then determined for each star image. A procedure for correcting the effects of a nonconstant background fog level on the plate is also demonstrated. Additional applications of the method are made in the appendix to determine the relation between the radius of a photographic star image and the star's magnitude, and to predict the IDP reading of the 'point of optimum density'.

Multiscaled exploration of coupled folding and binding of an intrinsically disordered molecular recognition element in measles virus nucleoprotein

PubMed Central

Wang, Yong; Chu, Xiakun; Longhi, Sonia; Roche, Philippe; Han, Wei; Wang, Erkang; Wang, Jin

2013-01-01

Numerous relatively short regions within intrinsically disordered proteins (IDPs) serve as molecular recognition elements (MoREs). They fold into ordered structures upon binding to their partner molecules. Currently, there is still a lack of in-depth understanding of how coupled binding and folding occurs in MoREs. Here, we quantified the unbound ensembles of the α-MoRE within the intrinsically disordered C-terminal domain of the measles virus nucleoprotein. We developed a multiscaled approach by combining a physics-based and an atomic hybrid model to decipher the mechanism by which the α-MoRE interacts with the X domain of the measles virus phosphoprotein. Our multiscaled approach led to remarkable qualitative and quantitative agreements between the theoretical predictions and experimental results (e.g., chemical shifts). We found that the free α-MoRE rapidly interconverts between multiple discrete partially helical conformations and the unfolded state, in accordance with the experimental observations. We quantified the underlying global folding–binding landscape. This leads to a synergistic mechanism in which the recognition event proceeds via (minor) conformational selection, followed by (major) induced folding. We also provided evidence that the α-MoRE is a compact molten globule-like IDP and behaves as a downhill folder in the induced folding process. We further provided a theoretical explanation for the inherent connections between “downhill folding,” “molten globule,” and “intrinsic disorder” in IDP-related systems. Particularly, we proposed that binding and unbinding of IDPs proceed in a stepwise way through a “kinetic divide-and-conquer” strategy that confers them high specificity without high affinity. PMID:24043820

Insights into the Molecular Mechanisms of Alzheimer's and Parkinson's Diseases with Molecular Simulations: Understanding the Roles of Artificial and Pathological Missense Mutations in Intrinsically Disordered Proteins Related to Pathology.

PubMed

Coskuner-Weber, Orkid; Uversky, Vladimir N

2018-01-24

Amyloid-β and α-synuclein are intrinsically disordered proteins (IDPs), which are at the center of Alzheimer's and Parkinson's disease pathologies, respectively. These IDPs are extremely flexible and do not adopt stable structures. Furthermore, both amyloid-β and α-synuclein can form toxic oligomers, amyloid fibrils and other type of aggregates in Alzheimer's and Parkinson's diseases. Experimentalists face challenges in investigating the structures and thermodynamic properties of these IDPs in their monomeric and oligomeric forms due to the rapid conformational changes, fast aggregation processes and strong solvent effects. Classical molecular dynamics simulations complement experiments and provide structural information at the atomic level with dynamics without facing the same experimental limitations. Artificial missense mutations are employed experimentally and computationally for providing insights into the structure-function relationships of amyloid-β and α-synuclein in relation to the pathologies of Alzheimer's and Parkinson's diseases. Furthermore, there are several natural genetic variations that play a role in the pathogenesis of familial cases of Alzheimer's and Parkinson's diseases, which are related to specific genetic defects inherited in dominant or recessive patterns. The present review summarizes the current understanding of monomeric and oligomeric forms of amyloid-β and α-synuclein, as well as the impacts of artificial and pathological missense mutations on the structural ensembles of these IDPs using molecular dynamics simulations. We also emphasize the recent investigations on residual secondary structure formation in dynamic conformational ensembles of amyloid-β and α-synuclein, such as β-structure linked to the oligomerization and fibrillation mechanisms related to the pathologies of Alzheimer's and Parkinson's diseases. This information represents an important foundation for the successful and efficient drug design studies.

Delivery of organics to Mars through asteroid and comet impacts

NASA Astrophysics Data System (ADS)

Frantseva, K.; Mueller, M.; van der Tak, F. F. S.; ten Kate, I. L.; Greenstreet, S.

2017-09-01

Preliminary results show that the asteroid-borne organic flux on Mars is comparable to the IPD rate; asteroids certainly cannot be neglected. Comets, on the other hand, contribute only 0.01% of the IDP-borne rate and can be neglected in the process of organic delivery to Mars.

Understanding disordered and unfolded proteins using single-molecule FRET and polymer theory.

PubMed

Hofmann, Hagen

2016-11-17

Understanding protein folding and the functional properties of intrinsically disordered proteins (IDPs) requires detailed knowledge of the forces that act in polypeptide chains. These forces determine the dimensions and dynamics of unfolded and disordered proteins and have been suggested to impact processes such as the coupled binding and folding of IDPs, or the rate of protein folding reactions. Much of the progress in understanding the physical and chemical properties of unfolded and intrinsically disordered polypeptide chains has been made possible by the recent developments in single-molecule fluorescence techniques. However, the interpretation of the experimental results requires concepts from polymer physics in order to be understood. Here, I review some of the theories used to describe the dimensions of unfolded polypeptide chains under varying solvent conditions together with their more recent application to experimental data.

Atomic force microscopy based nanoassay: a new method to study α-Synuclein-dopamine bioaffinity interactions

NASA Astrophysics Data System (ADS)

Corvaglia, Stefania; Sanavio, Barbara; Sorce, Barbara; Bosco, Alessandro; Sabella, Stefania; Pompa, Pierpaolo; Scoles, Giacinto; Casalis, Loredana

2015-03-01

Intrinsically Disordered Proteins (IDPs) are characterized by the lack of well-defined 3-D structure and show high conformational plasticity. For this reason, they are a strong challenge for the traditional characterization of structure, supramolecular assembly and biorecognition phenomena. We show here how the fine tuning of protein orientation on a surface turns useful in the reliable testing of biorecognition interactions of IDPs, in particular α-Synuclein. We exploited atomic force microscopy (AFM) for the selective, nanoscale confinement of α-Synuclein on gold to study the early stages of α-Synuclein aggregation and the effect of small molecules, like dopamine, on the aggregation process. Capitalizing on the high sensitivity of AFM topographic height measurements we determined, for the first time in the literature, the dissociation constant of dopamine- α-Synuclein adducts.

Atomic force microscopy based nanoassay: a new method to study α-Synuclein-dopamine bioaffinity interactions

NASA Astrophysics Data System (ADS)

Corvaglia, Stefania; Sanavio, Barbara; Hong Enriquez, Rolando P.; Sorce, Barbara; Bosco, Alessandro; Scaini, Denis; Sabella, Stefania; Pompa, Pier Paolo; Scoles, Giacinto; Casalis, Loredana

2014-06-01

Intrinsically Disordered Proteins (IDPs) are characterized by the lack of well-defined 3-D structure and show high conformational plasticity. For this reason, they are a strong challenge for the traditional characterization of structure, supramolecular assembly and biorecognition phenomena. We show here how the fine tuning of protein orientation on a surface turns useful in the reliable testing of biorecognition interactions of IDPs, in particular α-Synuclein. We exploited atomic force microscopy (AFM) for the selective, nanoscale confinement of α-Synuclein on gold to study the early stages of α-Synuclein aggregation and the effect of small molecules, like dopamine, on the aggregation process. Capitalizing on the high sensitivity of AFM topographic height measurements we determined, for the first time in the literature, the dissociation constant of dopamine-α-Synuclein adducts.

On the Origin of GEMS

NASA Technical Reports Server (NTRS)

Keller, L. P.; Messenger, S.

2004-01-01

GEMS (glass with embedded metal and sulfides) are a major component of anhydrous interplanetary dust particles (IDPs) their physical and chemical characteristics show marked similarities to contemporary interstellar dust. Recent oxygen isotopic measurements confirm that at least a small fraction (less than 5%) of GEMS are demonstrably presolar, while the remainder have ratios that are indistinguishable from solar values. GEMS with solar oxygen isotopic compositions either (1) had their isotopic compositions homogenized through processing in the interstellar medium (ISM), or (2) formed in the early solar system. Isotopic homogenization necessarily implies chemical homogenization, so (interstellar) GEMS compositions should reflect the average composition of dust in the local ISM. We performed a systematic examination of the bulk chemistry of GEMS in primitive IDPs in order to test this hypothesis.

Possible prevalence and transmission of acute respiratory tract infections caused by Streptococcus pneumoniae and Haemophilus influenzae among the internally displaced persons in tsunami disaster evacuation camps of Sri Lanka.

PubMed

Watanabe, Hiroshi; Batuwanthudawe, Ranjith; Thevanesam, Vasanthi; Kaji, Chiharu; Qin, Liang; Nishikiori, Nobuyuki; Saito, Wakana; Saito, Mariko; Watanabe, Kiwao; Oishi, Kazunori; Abeysinghe, Nihal; Kunii, Osamu

2007-01-01

The objective of this prospective study was to investigate the status of acute respiratory tract infections caused by Haemophilus influenzae and Streptococcus pneumoniae in tsunami disaster evacuation camps. Nasopharyngeal swabs (NP) of 324 internally displaced persons (IDP) in 3 different tsunami disaster evacuation camps of Sri Lanka were collected between March 18th and 20th, 2005, and analyzed for MIC, beta-lactamase production, serotypes, PCR and pulsed-field gel electrophoresis (PFGE). Many IDP had respiratory symptoms and the prevalence of cough and/or sputum was 84%, 70.5% and 64.7% in the three camps. Twenty-one H. influenzae from 20 IDP and 25 S. pneumoniae from 22 IDP were isolated from the NP. All H. influenzae isolates were nontypeable, and 5 were beta-lactamase producing. Seventeen pneumococci were susceptible, 5 showed intermediate resistance and 3 were fully resistant to penicillin G. Molecular analysis showed the 21 H. influenzae strains had 13 PFGE patterns and 25 pneumococci had 16 PFGE patterns. All 4 different PFGE patterns of H. influenzae strains were detected in a few IDP in camps 1 and 3, and 5 different PFGE patterns of serotype 3, 22A, 9A, 10A and 11A pneumococci were detected in a few IDP in camps 1 and 3. Our data indicate acute respiratory tract infections caused by various types of H. influenzae and S. pneumoniae appear to have been prevalent, some of which were potentially transmitted from person to person in tsunami disaster evacuation camps.

Nitrogen in Interplanetary Dust Particles

NASA Astrophysics Data System (ADS)

Keller, L. P.; Thomas, K. L.; Bradley, J. P.; McKay, D. S.

1995-09-01

Little is known about the abundance, distribution and chemical state of nitrogen in IDPs with the exceptions of the isotopic enrichment in 15N displayed by many particles [1-3], and the inferred association of nitrogen with polyaromatic hydrocarbons in some IDPs [4]. Like carbon, nitrogen is strongly fractionated among meteoritic materials and it is well known that the most primitive carbon-rich meteorites also tend to have high nitrogen abundances [5]. Nitrogen-bearing compounds are also a significant component of the carbonaceous material (CHON particles) sampled during the comet Halley encounter [e.g. 6]. We describe here the first reported detection and location of nitrogen concentrations in several IDPs using electron energy-loss spectroscopy. Three chondritic, anhydrous IDPs (L2011R11, L2008F13, and a fragment from L2006, cluster 14) were embedded in sulfur [7] and tranmission electron microscope (TEM) specimens were prepared by ultramicrotomy. The IDP thin sections were placed on copper TEM grids with SiO thin film substrates and analyzed using a JEOL 2010 TEM equipped with a thin-window energy-dispersive X-ray detector and a Gatan 666 parallel EELS spectrometer. We also analyzed W7027H14, a carbon-rich, chondritic-porous IDP that was embedded in epoxy. The EELS data from carbon-rich amorphous regions of the analyzed IDPs typically show a small, but distinct nitrogen edge at ~400 eV (Figure 1). The nitrogen is not homogeneously distributed in the carbonaceous material in the four IDPs analyzed to date, but occurs in "hot spots". However, these "hot spots" do not appear to be associated with a distinct N-bearing mineral (e.g. nitrides); the nitrogen is indigenous to the carbonaceous material in these IDPs. Although the quantitative N analyses using EELS are still in progress, the preliminary data from one IDP (L2011R11) indicates an upper N/C atom ratio of ~0.1, which is comparable to the chondritic value (N/C ~0.08, [8]). It should be noted however, that the

Outward foreign direct investments and home country's economic growth

NASA Astrophysics Data System (ADS)

Ciesielska, Dorota; Kołtuniak, Marcin

2017-09-01

The study examines the time stability of the causality direction and cross-correlations between the home country's economic growth and pace of growth of its outward foreign direct investment (OFDI) stocks within the complex system of the Polish national economy. The research has been performed in order to verify, using both the time and frequency domains time series analyses, if economic agents' long term decisions on outward foreign direct investments, leading to cross-border value chains and production fragmentation processes, are of adaptive or predictive character. Consequently, the aim was to check if the home country's economic growth leads the internationalization processes of domestic enterprises, which stays in line with Dunning's Investment Development Path (IDP) paradigm, or if these complex processes, thanks to entrepreneurs' ability to formulate relevant rational expectations, precede the home country's economic growth, which would be supported with the introduction of the policy on reinforcing the internationalization processes of domestic enterprises. The presence of the unidirectional economic growth-led internationalization, consistent with the IDP concept's base assumptions, has been ascertained by the results of the short term Granger causality tests. Nevertheless, the results of the wavelet analyses, supported with the results of the econometric block exogeneity long term causality Wald tests, have revealed that in the long term the OFDI stocks' growth permanently precedes the home country's economic growth, which stays in the unequivocal contrast with the IDP paradigm's premises, as well as with the indicated above short term Granger causality tests' outcomes and indicates that economic agents' choices are not strictly of adaptive but also of predictive character, which influences the current state of knowledge on economic complex systems' characteristics. Such a result is of a great importance in the light of the existence of the significant

Dynamics of partially folded and unfolded proteins investigated with quasielastic neutron spectroscopy

NASA Astrophysics Data System (ADS)

Stadler, Andreas M.

2018-05-01

Molecular dynamics in proteins animate and play a vital role for biologically relevant processes of these biomacromolecules. Quasielastic incoherent neutron scattering (QENS) is a well-suited experimental method to study protein dynamics from the picosecond to several nanoseconds and in the Ångström length-scale. In QENS experiments of protein solutions hydrogens act as reporters for the motions of methyl groups or amino acids to which they are bound. Neutron Spin-Echo spectroscopy (NSE) offers the highest energy resolution in the field of neutron spectroscopy and allows the study of slow collective motions in proteins up to several hundred nanoseconds and in the nanometer length-scale. In the following manuscript I will review recent studies that stress the relevance of molecular dynamics for protein folding and for conformational transitions of intrinsically disordered proteins (IDPs). During the folding collapse the protein is exploring its accessible conformational space via molecular motions. A large flexibility of partially folded and unfolded proteins, therefore, is mandatory for rapid protein folding. IDPs are a special case as they are largely unstructured under physiological conditions. A large flexibility is a characteristic property of IDPs as it allows, for example, the interaction with various binding partners or the rapid response to different conditions.

TERENA eScience PKI

NASA Astrophysics Data System (ADS)

Sova, Milan

Several National Research and Education Networks associated in TERENA have joined their efforts to build a shared PKI able to serve potentially millions of users from their constituency. The TCS eScience Personal CA takes advantage of national identity federations to facilitate user identity vetting and enrollment procedures. The system uses identity management systems (IdMS) at participating institutions to perform the functions of registration authorities. The certificate enrollment application acts as a SAML Service Provider relying on information provided by IdMS performing as SAML Identity Providers (IdP). When applying for a personal certificate, users authenticate at their home IdP using credentials they normally use to access local services. The IdP controls the certificate issuance process by releasing SAML attributes specifying the user's eligibility for the service and the information to be included in the certificate such as the user's name and email address. The TCS eScience Personal CA is part of the TERENA Certificate Service that uses a commercial PKI provider. Outsourcing the actual CA machinery to a specialized company results in professional-level services such as CRL and OCSP management. The paper describes the legal, organizational and technical aspects of the TCS eScience PKI.

Improving the Quality of Life of Persons with Intellectual Disabilities Through ICTs.

PubMed

Ferreras, Alberto; Poveda, Rakel; Quílez, Manuel; Poll, Nuria

2017-01-01

Removing barriers to accessing Information and Communication Technologies (ICTs) by Persons with Intellectual Disabilities (IDPs) is crucial. Being excluded from ICTs implies being shut down from the information society, but also from accessing essential public services, as well as from the opportunity of living an independent life. The IdICT project has the general objective of increasing the competences of IDPs, their families and the professionals that work with them to exploit ICTs with a Quality of Life approach. To do that, a training platform and a training program has been developed and tested by IDPs, relatives and professionals in six European Countries.

NPOESS Preparatory Project (NPP) Science Overview

NASA Technical Reports Server (NTRS)

Butler, James J.

2011-01-01

NPP Instruments are: (1) well understood thanks to instrument comprehensive test, characterization and calibration programs. (2) Government team ready for October 25 launch followed by instrument activation and Intensive Calibration/Validation (ICV). NPP Data Products preliminary work includes: (1) JPSS Center for Satellite Applications and Research (STAR) team ready to support NPP ICV and operational data products. (2) NASA NPP science team ready to support NPP ICV and EOS data continuity.

Insomnia in a displaced population is related to war-associated remembered stress.

PubMed

Basishvili, Tamar; Eliozishvili, Marine; Maisuradze, Lia; Lortkipanidze, Nani; Nachkebia, Nargiz; Oniani, Tengiz; Gvilia, Irma; Darchia, Nato

2012-08-01

Although traumatic events are presumed to cause sleep disturbances, particularly insomnia, sleep in populations subjected to forced displacement has received little attention. The present study examined the prevalence of insomnia and associated factors in internally displaced persons (IDPs) from Abkhazia 15 years after displacement to Tbilisi. Detailed subjective information about sleep-wake habits, sleep-related and stress-related parameters were obtained from 87 IDPs categorized into good sleepers and insomniacs. The Insomnia Severity Index, Perceived Stress Scale and Beck Depression Inventory were administered. The incidence of insomnia was 41.4%. The majority of insomniacs strongly believed that war-related stress accounted for the onset of their insomnia. Stepwise regression (95% confidence interval) revealed four variables significantly associated with insomnia status: self-estimated influence of war related stress (odds ratio (OR) = 2.51), frequency of nightmares (OR = 1.6), Perceived Stress Scale score (OR = 1.14) and Beck Depression Inventory score (OR = 1.12). Insomnia in IDPs was strongly related to war-associated remembered stress. ‛Over thinking' about major stress exposure enhanced IDPs' vulnerability to insomnia. These findings have implications for the management of insomnia and associated impairment of daytime functioning in IDPs. Copyright © 2011 John Wiley & Sons, Ltd.

The measurement of trace elements in interplanetary dust and cometary particles by ultra-high sensitivity INAA

NASA Technical Reports Server (NTRS)

Zolensky, M. E.; Lindstrom, David J.; Lindstrom, Richard M.; Lindstrom, M. M.

1989-01-01

Today the major elemental composition of interplanetary dust particles (IDPs) is routinely determined in many laboratories. These and mineralogical studies have revealed the presence of at least two major types of IDPs, chondritic and refractory. Preliminary results of a successful attempt to determine abundances of a large suite of trace elements from both chondritic and refractory IDPs are reported. The analytical procedure can be used in the grain-by-grain analysis of returned cometary samples. Chondritic and refractory IDPs are characterized by standard scanning electron microscopy and energy dispersive x ray spectroscopy (SEM-EDX) techniques. With this system, detection limits for many elements are well below picogram levels, and some approach femtogram levels. This technique is non-destructive, although some sample handling is required, so particles can be analyzed by other techniques after instrument neutron activation analysis (INAA) is completed. Data is presently being reduced from the analyses of 7 IDPs. These are U2015E10, U2015F1, W7029-A2, W7029-A3, W7013A8, LACl (all chondritic) and 705 (refractory). So far, 17 different major and trace elements were detected and measured in these particles, including rare earths and some very volatile elements (Br and Zn).

Conformational transition of κ-casein in micellar environment: Insight from the tryptophan fluorescence

NASA Astrophysics Data System (ADS)

Mishra, Smruti; Meher, Geetanjali; Chakraborty, Hirak

2017-11-01

Intrinsically disordered proteins (IDPs) are under intense analysis due to their structural flexibility and importance in biological functions. Minuscule modulation in the microenvironment induces significant conformational changes in IDPs, and these non-native conformations of the IDPs often induce aggregation and cause cell death. Changes in the membrane composition often change the microenvironment, which promote conformational change and aggregation of IDPs. κ-Casein, an important milk protein, belongs to the class of IDPs containing net negative charges. In this present work, we have studied the interaction of κ-casein with cetyltrimethyl ammonium bromide (CTAB), a positively charged surfactant, utilizing various steady state fluorescence, time-resolved fluorescence and circular dichroism spectroscopy. Our results clearly indicate that κ-casein undergoes at least two conformational transitions in presence of various concentrations of CTAB. The intrinsically disordered κ-casein assumes a partially folded conformation at lower concentration of CTAB, which adopts an unstructured conformation at higher concentration of CTAB. The partially folded conformation of κ-casein at a lower CTAB concentration might be induced by the favorable electrostatic interaction between the positively charged surfactant headgroup and net negative charges of the protein, whereas surfactant nature of CTAB is being pronounced at higher concentration of CTAB.

Proteins without unique 3D structures: biotechnological applications of intrinsically unstable/disordered proteins.

PubMed

Uversky, Vladimir N

2015-03-01

Intrinsically disordered proteins (IDPs) and intrinsically disordered protein regions (IDPRs) are functional proteins or regions that do not have unique 3D structures under functional conditions. Therefore, from the viewpoint of their lack of stable 3D structure, IDPs/IDPRs are inherently unstable. As much as structure and function of normal ordered globular proteins are determined by their amino acid sequences, the lack of unique 3D structure in IDPs/IDPRs and their disorder-based functionality are also encoded in the amino acid sequences. Because of their specific sequence features and distinctive conformational behavior, these intrinsically unstable proteins or regions have several applications in biotechnology. This review introduces some of the most characteristic features of IDPs/IDPRs (such as peculiarities of amino acid sequences of these proteins and regions, their major structural features, and peculiar responses to changes in their environment) and describes how these features can be used in the biotechnology, for example for the proteome-wide analysis of the abundance of extended IDPs, for recombinant protein isolation and purification, as polypeptide nanoparticles for drug delivery, as solubilization tools, and as thermally sensitive carriers of active peptides and proteins. Copyright © 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

DIBS: a repository of disordered binding sites mediating interactions with ordered proteins.

PubMed

Schad, Eva; Fichó, Erzsébet; Pancsa, Rita; Simon, István; Dosztányi, Zsuzsanna; Mészáros, Bálint

2018-02-01

Intrinsically Disordered Proteins (IDPs) mediate crucial protein-protein interactions, most notably in signaling and regulation. As their importance is increasingly recognized, the detailed analyses of specific IDP interactions opened up new opportunities for therapeutic targeting. Yet, large scale information about IDP-mediated interactions in structural and functional details are lacking, hindering the understanding of the mechanisms underlying this distinct binding mode. Here, we present DIBS, the first comprehensive, curated collection of complexes between IDPs and ordered proteins. DIBS not only describes by far the highest number of cases, it also provides the dissociation constants of their interactions, as well as the description of potential post-translational modifications modulating the binding strength and linear motifs involved in the binding. Together with the wide range of structural and functional annotations, DIBS will provide the cornerstone for structural and functional studies of IDP complexes. DIBS is freely accessible at http://dibs.enzim.ttk.mta.hu/. The DIBS application is hosted by Apache web server and was implemented in PHP. To enrich querying features and to enhance backend performance a MySQL database was also created. dosztanyi@caesar.elte.hu or bmeszaros@caesar.elte.hu. Supplementary data are available at Bioinformatics online. © The Author 2017. Published by Oxford University Press.

Dust in the Solar System - Properties and Origins

NASA Technical Reports Server (NTRS)

Messenger, Scott; Keller, Lindsay; Nakamura-Messenger, Keiko

2013-01-01

Interplanetary dust pervades the inner Solar System, giving rise to a prominent glow above the horizon at sunrise and sunset known as the zodiacal light. This dust derives from the disintegration of comets as they approach the Sun and from collisions among main-belt asteroids. The Earth accretes roughly 4x10(exp 6) kg/year of 1 - 1,000 micron dust particles as they spiral into the Sun under the influence of Poynting-Robertson drag and solar wind drag. Samples of these grains have been collected from deep sea sediments, Antarctic ice and by high-altitude aircraft and balloon flights. Interplanetary dust particles (IDPs) collected in the stratosphere have been classified by their IR spectra into olivine, pyroxene, and hydrated silicate-dominated classes. Most IDPs have bulk major and minor element abundances that are similar to carbonaceous chondrite meteorites. Hydrated silicate-rich IDPs are thought to derive from asteroids based on their mineralogy and low atmospheric entry velocities estimated from peak temperatures reached during atmospheric entry. Anhydrous IDPs are typically aggregates of 0.1 - approx. 1 micron Mg-rich olivine and pyroxene, amorphous silicates (GEMS), Fe, Nisulfides and rare spinel and oxides bound together by carbonaceous material. These IDPs are often argued to derive from comets based on compositional similarities and high atmospheric entry velocities that imply high eccentricity orbits. Infrared spectra obtained from anhydrous IDPs closely match remote IR spectra obtained from comets. The most primitive (anhydrous) IDPs appear to have escaped the parent-body thermal and aqueous alteration that has affected meteorites. These samples thus consist entirely of grains that formed in the ancient solar nebula and pre-solar interstellar and circumstellar environments. Isotopic studies of IDPs have identified silicate stardust grains that formed in the outflows of red giant and asymptotic giant branch stars and supernovae]. These stardust grains

AIRS-Light Instrument Concept and Critical Technology Development

NASA Technical Reports Server (NTRS)

Maschhoff, Kevin

2001-01-01

Understanding Earth's climate, atmospheric transport mechanisms, and the hydrologic cycle requires a precise knowledge of global atmospheric circulation, temperature profiles, and water vapor distribution. The accuracy of advanced sounders such as AIRS/AMSU/HSB on NASA's Aqua spacecraft can match radiosonde accuracy. It is essential to fold those capabilities fully into the NPOESS, enabling soundings of radiosonde accuracy, every 6 hours around the globe on an operational basis. However, the size, mass, power demands, and thermal characteristics of the Aqua sounding instrument suite cannot be accommodated on the NPOESS spacecraft. AIRS-Light is an instrument concept, developed under the Instrument Incubator Program, which provides IR sounding performance identical to the AIRS instrument, but uses advances in HgCdTe FPA technology and pulse tube cooler technology, as well as design changes to dramatically reduce the size, mass, and power demand, allowing AIRS-Light to meet all NPOESS spacecraft interface requirements. The instrument concept includes substantial re-use of AIRS component designs, including the complex AIRS FPA, to reduce development risk and cost. The AIRS-Light Instrument Incubator program fostered the development of photovoltaic-mode HgCdTe detector array technology for the 13.5-15.4 micron band covered by photoconductive-mode HgCdTe arrays in AIRS, achieved state of the art results in this band, and substantially reduced the development risk for this last new technology needed for AIRS-Light implementation, A demonstration of a prototype 14.5-15.4 micron band IRFPA in a reduced heat-load dewar together with the IMAS pulse tube cryocooler is in progress.

Analysis of organic grain coatings in primitive interplanetary dust particles: Implications for the origin of Solar System organic matter

NASA Astrophysics Data System (ADS)

Flynn, George

Analysis of organic grain coatings in primitive interplanetary dust particles: Implications for the origin of Solar System organic matter Chondritic, porous interplanetary dust particles (CP IDPs), the most primitive samples of extraterrestrial material available for laboratory analysis [1], are unequilibrated aggregates of mostly submicron, anhydrous grains of a diverse mineralogy. They contain organic matter not produced by parent body aqueous processing [2], some carrying H and N isotopic anomalies consistent with molecular cloud or outer Solar System material [3]. Scanning Transmission X-Ray Microscope (STXM) imaging at the C K-edge shows the individual grains in 10 micron aggregate CP IDPs are coated by a layer of carbonaceous material 100 nm thick. This structure implies a three-step formation sequence. First, individual grains condensed from the cooling nebular gas. Then complex, refractory organic molecules covered the surfaces of the grains either by deposition, formation in-situ, or a combination of both processes. Finally, the grains collided and stuck together forming the first dust-size material in the Solar System. Ultramicrotome sections, 70 to 100 nm thick were cut from several CP IDPs, embedded in elemental S to avoid exposure to C-based embedding media. X-ray Absorption Near Edge Structure (XANES) spectra were derived from image stacks obtained using a STXM. "Cluster analysis" was used to compare the C-XANES spectra from each of the pixels in an image stack and identify pixels exhibiting similar spectra. When applied to a CP IDP, cluster analysis identifies most carbonaceous grain coatings in a particle as having similar C-XANES spectra. Two processes are commonly suggested in the literature for production of organic grain coatings. The similarity in thickness and C-XANES spectra of the coatings on different minerals in the same IDP indicates the first, mineral specific catalysis, was not the process that produced these organic rims. Our results

The CCC Institutional Development Program: Organizational Change from the Chief Executive's Perspective.

ERIC Educational Resources Information Center

Ellison, Nolen M.; Eadie, Douglas C.

Cuyahoga Community College's three-phase Institutional Development Program (IDP) was established in January, 1975, to provide systematic and uniform college-wide planning and management procedures. This report first discusses the social forces which led the college to undertake the IDP; identifies the long-range planning, governance, and…

Individual Development Plans as Governance Tools--Changed Governance of Teachers' Work

ERIC Educational Resources Information Center

Parding, Karolina; Liljegren, Andreas

2017-01-01

Auditing, accountability, and transparency are concepts that greatly impact the working conditions of today's public sector professionals, including teachers. Documentation requirements have been on the increase for some time, which can be seen in the education sector's Individual Development Plans (IDPs), for example. These IDPs are pedagogical…

Looking at the Disordered Proteins through the Computational Microscope.

PubMed

Das, Payel; Matysiak, Silvina; Mittal, Jeetain

2018-05-23

Intrinsically disordered proteins (IDPs) have attracted wide interest over the past decade due to their surprising prevalence in the proteome and versatile roles in cell physiology and pathology. A large selection of IDPs has been identified as potential targets for therapeutic intervention. Characterizing the structure-function relationship of disordered proteins is therefore an essential but daunting task, as these proteins can adapt transient structure, necessitating a new paradigm for connecting structural disorder to function. Molecular simulation has emerged as a natural complement to experiments for atomic-level characterizations and mechanistic investigations of this intriguing class of proteins. The diverse range of length and time scales involved in IDP function requires performing simulations at multiple levels of resolution. In this Outlook, we focus on summarizing available simulation methods, along with a few interesting example applications. We also provide an outlook on how these simulation methods can be further improved in order to provide a more accurate description of IDP structure, binding, and assembly.

Intrinsic disorder mediates the diverse regulatory functions of the Cdk inhibitor p21

PubMed Central

Wang, Yuefeng; Fisher, John C.; Mathew, Rose; Ou, Li; Otieno, Steve; Sublett, Jack; Xiao, Limin; Chen, Jianhan; Roussel, Martine F.; Kriwacki, Richard W.

2011-01-01

Traditionally, well-defined three-dimensional structure was thought to be essential for protein function. However, myriad biological functions are performed by highly dynamic, intrinsically disordered proteins (IDPs). IDPs often fold upon binding their biological targets and frequently exhibit “binding diversity” by targeting multiple ligands. We sought to understand the physical basis of IDP binding diversity and herein report that the cyclin-dependent kinase (Cdk) inhibitor, p21Cip1, adaptively binds to and inhibits the various Cdk/cyclin complexes that regulate eukaryotic cell division. Based on results from NMR spectroscopy, and biochemical and cellular assays, we show that structural adaptability of a helical sub-domain within p21 termed LH enables two other sub-domains termed D1 and D2 to specifically bind conserved surface features of the cyclin and Cdk subunits, respectively, within otherwise structurally distinct Cdk/cyclin complexes. Adaptive folding upon binding is likely to mediate the diverse biological functions of the thousands of IDPs present in eukaryotes. PMID:21358637

Multi-level partnerships to promote health services among internally displaced in eastern Burma.

PubMed

Mahn, M; Maung, C; Oo, E K Shwe; Smith, L; Lee, C I; Whichard, E; Neumann, C; Richards, A K; Mullany, L C; Kuiper, H; Lee, T J

2008-01-01

Ethnic populations in eastern Burma are the target of military policies that result in forced labour, destruction of food supplies, and massive forced displacement. Despite international assistance to Burmese refugees along the Thai-Burma border, traditional humanitarian models have failed to reach these internally displaced persons (IDPs) within Burma. Nevertheless, through the cultivation of a model (cross border local-global partnerships) 300,000 IDPs in eastern Burma now receive critical health services where, otherwise, there would be none. We describe key elements of the partnership model's genesis in eastern Burma. The role of the local partner, Backpack Health Worker Team (BPHWT), is highlighted for its indigenous access to the IDP populations and its maintenance of programmatic autonomy. These local elements are potentiated by international support for technical assistance, training, resources, and advocacy. International policy and investment should prioritize support of locally-driven health initiatives that utilize local-global partnerships to reach not only IDPs but also other war-torn or traditionally inaccessible populations worldwide.

Mineralogy of interplanetary dust particles from the 'olivine' infrared class

NASA Technical Reports Server (NTRS)

Christoffersen, R.; Buseck, P. R.

1986-01-01

Analytical electron microscopy observations establish that olivine is abundant and the predominant silicate phase in three interplanetary dust particles (IDPs) from the 'olivine' infrared spectra category. Two of the particles have microstructures resembling those of most nonhydrous chondritic IDPs, consisting of micron to submicron grains together with a matrix composed of amorphous carbonaceous material and sub-500 A grains. In addition to olivine these particles respectively contain enstatite and magnetite, and pentlandite plus Ca-rich clinopyroxene. The third IDP consists mostly of olivine and pyrrhotite with little or no matrix material. Olivine grains in this particle contain prominent solar-flare ion tracks with densities corresponding to a space-exposure age between 1000 to 100,000 years. Although the three particles have olivine-rich mineralogies in common, other aspects of their mineralogies and microstructures suggest that they experienced different formation histories. The differences between the particles indicate that the olivine infrared spectral category is a diverse collection of IDPs that probably incorporates several genetic groups.

Looking at the Disordered Proteins through the Computational Microscope

PubMed Central

2018-01-01

Intrinsically disordered proteins (IDPs) have attracted wide interest over the past decade due to their surprising prevalence in the proteome and versatile roles in cell physiology and pathology. A large selection of IDPs has been identified as potential targets for therapeutic intervention. Characterizing the structure–function relationship of disordered proteins is therefore an essential but daunting task, as these proteins can adapt transient structure, necessitating a new paradigm for connecting structural disorder to function. Molecular simulation has emerged as a natural complement to experiments for atomic-level characterizations and mechanistic investigations of this intriguing class of proteins. The diverse range of length and time scales involved in IDP function requires performing simulations at multiple levels of resolution. In this Outlook, we focus on summarizing available simulation methods, along with a few interesting example applications. We also provide an outlook on how these simulation methods can be further improved in order to provide a more accurate description of IDP structure, binding, and assembly.

Effect of multilevel lumbar disc arthroplasty on the operative- and adjacent-level kinematics and intradiscal pressures: an in vitro human cadaveric assessment.

PubMed

Dmitriev, Anton E; Gill, Norman W; Kuklo, Timothy R; Rosner, Michael K

2008-01-01

With lumbar arthroplasty gaining popularity, limited data are available highlighting changes in adjacent-level mechanics after multilevel procedures. Compare operative- and adjacent-segment range of motion (ROM) and intradiscal pressures (IDPs) after two-level arthroplasty versus circumferential arthrodesis. Cadaveric biomechanical study. Ten human cadaveric lumbar spines were used in this investigation. Biomechanical testing was performed according to a hybrid testing protocol using an unconstrained spine simulator under axial rotation (AR), flexion extension (FE), and lateral-bending (LB) loading. Specimens were tested in the following order: 1) Intact, 2) L3-L5 total disc replacement (TDR), 3) L3-L5 anterior interbody cages+pedicle screws. IDP was recorded at proximal and distal adjacent levels and normalized to controls (%intact). Full ROM was monitored at the operative and adjacent levels and reported in degrees. Kinematics assessment revealed L3-L5 ROM reduction after both reconstructions versus intact controls (p < .05). However, global quality of segmental motion distributed over L2-S1 was preserved in the arthroplasty group but was significantly altered after circumferential fixation. Furthermore, adjacent-level ROM was increased for the arthrodesis group under LB at both segments and during AR at L2-L3 relative to controls (p < .05). FE did not reveal any intergroup statistical differences. Nonetheless, after arthrodesis IDPs were increased proximally under all three loading modalities, whereas distally a significant IDP rise was noted during AR and LB (p < .05). No statistical differences in either biomechanical parameter were recorded at the adjacent levels between intact control and TDR groups. Our results indicate no significant adjacent-level biomechanical changes between arthroplasty and control groups. In contrast, significant alterations in ROM and IDP were recorded both proximally (ROM=LB & AR; IDP=AR, FE, LB) and distally (ROM=LB; IDP=AR & LB

Training and deployment of lay refugee/internally displaced persons to provide basic health services in camps: a systematic review.

PubMed

Ehiri, John E; Gunn, Jayleen K L; Center, Katherine E; Li, Ying; Rouhani, Mae; Ezeanolue, Echezona E

2014-01-01

Training of lay refugees/internally displaced persons (IDPs) and deploying them to provide basic health services to other women, children, and families in camps is perceived to be associated with public health benefits. However, there is limited evidence to support this hypothesis. To assess the effects of interventions to train and deploy lay refugees and/or IDPs for the provision of basic health service to other women, children, and families in camps. PubMed, Science and Social Science Citation Indices, PsycINFO, EMBASE, POPLINE, CINAHL, and reference lists of relevant articles were searched (from inception to June 30, 2014) with the aim of identifying studies that reported the effects of interventions that trained and deployed lay refugees and/or IDPs for the provision of basic health service to other women, children, and families in camps. Two investigators independently reviewed all titles and abstracts to identify potentially relevant articles. Discrepancies were resolved by repeated review, discussion, and consensus. Study quality assessment was undertaken using standard protocols. Ten studies (five cross-sectional, four pre-post, and one post-test only) conducted in Africa (Guinea and Tanzania), Central America (Belize), and Asia (Myanmar) were included. The studies demonstrated some positive impact on population health associated with training and deployment of trained lay refugees/IDPs as health workers in camps. Reported effects included increased service coverage, increased knowledge about disease symptoms and prevention, increased adoption of improved treatment seeking and protective behaviors, increased uptake of services, and improved access to reproductive health information. One study, which assessed the effect of peer refugee health education on sexual and reproductive health, did not demonstrate a marked reduction in unintended pregnancies among refugee/IDP women. Although available evidence suggests a positive impact of training and deployment

Sequence charge decoration dictates coil-globule transition in intrinsically disordered proteins.

PubMed

Firman, Taylor; Ghosh, Kingshuk

2018-03-28

We present an analytical theory to compute conformations of heteropolymers-applicable to describe disordered proteins-as a function of temperature and charge sequence. The theory describes coil-globule transition for a given protein sequence when temperature is varied and has been benchmarked against the all-atom Monte Carlo simulation (using CAMPARI) of intrinsically disordered proteins (IDPs). In addition, the model quantitatively shows how subtle alterations of charge placement in the primary sequence-while maintaining the same charge composition-can lead to significant changes in conformation, even as drastic as a coil (swelled above a purely random coil) to globule (collapsed below a random coil) and vice versa. The theory provides insights on how to control (enhance or suppress) these changes by tuning the temperature (or solution condition) and charge decoration. As an application, we predict the distribution of conformations (at room temperature) of all naturally occurring IDPs in the DisProt database and notice significant size variation even among IDPs with a similar composition of positive and negative charges. Based on this, we provide a new diagram-of-states delineating the sequence-conformation relation for proteins in the DisProt database. Next, we study the effect of post-translational modification, e.g., phosphorylation, on IDP conformations. Modifications as little as two-site phosphorylation can significantly alter the size of an IDP with everything else being constant (temperature, salt concentration, etc.). However, not all possible modification sites have the same effect on protein conformations; there are certain "hot spots" that can cause maximal change in conformation. The location of these "hot spots" in the parent sequence can readily be identified by using a sequence charge decoration metric originally introduced by Sawle and Ghosh. The ability of our model to predict conformations (both expanded and collapsed states) of IDPs at a high

An exploration of social determinants of health amongst internally displaced persons in northern Uganda

PubMed Central

2009-01-01

Social determinants of health describe the conditions in which people are born, grow, live, work and age and their influence on health. These circumstances are shaped by the distribution of money, power and resources at global, national and local levels, which are themselves influenced by policy choices. Armed conflict and forced displacement are important influences on the social determinants of health. There is limited evidence on the social determinants of health of internally displaced persons (IDPs) who have been forced from their homes due to armed conflict but remain within the borders of their country. The aim of this study was to explore the social determinants of overall physical and mental health of IDPs, including the response strategies used by IDPs to support their health needs. Northern Uganda was chosen as a case-study, and 21 face-to-face semi-structured interviews with IDPs were conducted in fifteen IDP camps between November and December 2006. The findings indicated a number of key social determinants. Experiencing traumatic events could cause "over thinking" which in turn could lead to "madness" and physical ailments. Respondents also attributed "over thinking" to the spirit (cen) of a killed person returning to disturb its killer. Other social determinants included overcrowding which affected physical health and contributed to an emotional sense of loss of freedom; and poverty and loss of land which affected physical health from lack of food and income, and mental health because of worry and uncertainty. Respondents also commented on how the conflict and displacement and led to changes in social and cultural norms such as increased "adultery", "defilement", and "thieving". Response strategies included a combination of biopsychosocial health services, traditional practices, religion, family and friends, and isolating. This study supports work exploring the political, environmental, economic, and socio-cultural determinants of health of IDPs

Sequence charge decoration dictates coil-globule transition in intrinsically disordered proteins

NASA Astrophysics Data System (ADS)

Firman, Taylor; Ghosh, Kingshuk

2018-03-01

We present an analytical theory to compute conformations of heteropolymers—applicable to describe disordered proteins—as a function of temperature and charge sequence. The theory describes coil-globule transition for a given protein sequence when temperature is varied and has been benchmarked against the all-atom Monte Carlo simulation (using CAMPARI) of intrinsically disordered proteins (IDPs). In addition, the model quantitatively shows how subtle alterations of charge placement in the primary sequence—while maintaining the same charge composition—can lead to significant changes in conformation, even as drastic as a coil (swelled above a purely random coil) to globule (collapsed below a random coil) and vice versa. The theory provides insights on how to control (enhance or suppress) these changes by tuning the temperature (or solution condition) and charge decoration. As an application, we predict the distribution of conformations (at room temperature) of all naturally occurring IDPs in the DisProt database and notice significant size variation even among IDPs with a similar composition of positive and negative charges. Based on this, we provide a new diagram-of-states delineating the sequence-conformation relation for proteins in the DisProt database. Next, we study the effect of post-translational modification, e.g., phosphorylation, on IDP conformations. Modifications as little as two-site phosphorylation can significantly alter the size of an IDP with everything else being constant (temperature, salt concentration, etc.). However, not all possible modification sites have the same effect on protein conformations; there are certain "hot spots" that can cause maximal change in conformation. The location of these "hot spots" in the parent sequence can readily be identified by using a sequence charge decoration metric originally introduced by Sawle and Ghosh. The ability of our model to predict conformations (both expanded and collapsed states) of IDPs at

Cometary dust in Antarctic ice and snow: Past and present chondritic porous micrometeorites preserved on the Earth's surface

NASA Astrophysics Data System (ADS)

Noguchi, Takaaki; Ohashi, Noriaki; Tsujimoto, Shinichi; Mitsunari, Takuya; Bradley, John P.; Nakamura, Tomoki; Toh, Shoichi; Stephan, Thomas; Iwata, Naoyoshi; Imae, Naoya

2015-01-01

Chondritic porous interplanetary dust particles (CP IDPs) collected in the stratosphere are regarded as possibly being cometary dust, and are therefore the most primitive solar system material that is currently available for analysis in laboratories. In this paper we report the discovery of more than 40 chondritic porous micrometeorites (CP MMs) in the surface snow and blue ice of Antarctica, which are indistinguishable from CP IDPs. The CP MMs are botryoidal aggregates, composed mainly of sub-micrometer-sized constituents. They contain two components that characterize them as CP IDPs: enstatite whiskers and GEMS (glass with embedded metal and sulfides). Enstatite whiskers appear as <2-μm-long acicular objects that are attached on, or protrude from the surface, and when included in the interior of the CP MMs are composed of a unit-cell scale mixture of clino- and ortho-enstatite, and elongated along the [100] direction. GEMS appear as 100-500 nm spheroidal objects containing <50 nm Fe-Ni metal and Fe sulfide. The CP MMs also contain low-iron-manganese-enriched (LIME) and low-iron-chromium-enriched (LICE) ferromagnesian silicates, kosmochlor (NaCrSi2O6)-rich high-Ca pyroxene, roedderite (K, Na)2Mg5Si12O30, and carbonaceous nanoglobules. These components have previously been discovered in primitive solar system materials such as the CP IDPs, matrices of primitive chondrites, phyllosilicate-rich MMs, ultracarbonaceous MMs, and cometary particles recovered from the 81P/Wild 2 comet. The most outstanding feature of these CP MMs is the presence of kosmochlor-rich high-Ca pyroxene and roedderite, which suggest that they have building blocks in common with CP IDPs and cometary dust particles and therefore suggest a possible cometary origin of both CP MMs and CP IDPs. It is therefore considered that CP MMs are CP IDPs that have fallen to Earth and have survived the terrestrial environment.

Genome-Wide Prediction of Intrinsic Disorder; Sequence Alignment of Intrinsically Disordered Proteins

ERIC Educational Resources Information Center

Midic, Uros

2012-01-01

Intrinsic disorder (ID) is defined as a lack of stable tertiary and/or secondary structure under physiological conditions in vitro. Intrinsically disordered proteins (IDPs) are highly abundant in nature. IDPs possess a number of crucial biological functions, being involved in regulation, recognition, signaling and control, e.g. their functional…

Academics as Part-Time Marketers in University Offshore Programs: An Exploratory Study

ERIC Educational Resources Information Center

Poole, David; Ewan, Craig

2010-01-01

Australian universities maintain almost 900 offshore programs delivered to more than 100 000 students, primarily in the nations of Singapore, Malaysia, China, and Hong Kong (Universities Australia, 2009; IDP, 2009a). Although offshore students comprise an estimated 30 per cent of international student enrolments at Australian universities (IDP,…

Abstracts for the 54th Annual Meeting of the Meteoritical Society

NASA Technical Reports Server (NTRS)

1991-01-01

Abstracts of the papers presented at 54th Annual Meeting of the Meteoritic Society are compiled. The following subject areas are covered: Antarctic meteorites; nebula and parent body processing; primary and secondary SNC parent planet processes; enstatite chondrites and aubrites; achondrite stew; refractory inclusions; meteorite exposure ages and sizes; interstellar/meteorite connections; lunar origins, processes and meteorites; craters, cratering and tektites; cretaceous-tertiary impact(s); IDPs (LDEF, stratosphere, Greenland and Antarctica); chondrules; and chondrites.

Vaccination coverage survey versus administrative data in the assessment of mass yellow fever immunization in internally displaced persons--Liberia, 2004.

PubMed

Huhn, Gregory D; Brown, Jennifer; Perea, William; Berthe, Adama; Otero, Hansel; LiBeau, Genevieve; Maksha, Nuhu; Sankoh, Mohammed; Montgomery, Susan; Marfin, Anthony; Admassu, Mekonnen

2006-02-06

Yellow fever (YF) is a mosquito-borne vaccine-preventable disease with high mortality. In West Africa, low population immunity increases the risk of epidemic transmission. A cluster survey was conducted to determine the effectiveness of a mass immunization campaign using 17D YF vaccine in internally displaced person (IDP) camps following a reported outbreak of YF in Liberia in February 2004. Administrative data of vaccination coverage were reviewed. A cluster sample size was determined among 17,384 shelters using an 80% vaccination coverage threshold. A questionnaire eliciting demographic information, household size, and vaccination status was distributed to randomly selected IDPs. Data were analyzed to compare vaccination coverage rates of administrative versus survey data. Among 87,000 persons estimated living in IDP camps, administrative data recorded 49,395 (57%) YF vaccinated persons. A total of 237 IDPs were surveyed. Of survey respondents, 215 (91.9%, 95% CI 88.4-95.4) reported being vaccinated during the campaign and 196 (83.5%, 95% CI 78.6-88.5) possessed a valid campaign vaccination card. The median number of IDPs living in a shelter was 4 (range, 1-8) and 69,536 persons overall were estimated to be living in IDP camps. Coverage rates from a rapid survey exceeded 90% by self-report and 80% by evidence of a vaccination card, indicating that the YF immunization campaign was effective. Survey results suggested that administrative data overestimated the camp population by at least 20%. An emergency, mop-up vaccination campaign was avoided. Coverage surveys can be vital in the evaluation of emergency vaccination campaigns by influencing both imminent and future immunization strategies.

Soft interactions and volume exclusion by polymeric crowders can stabilize or destabilize transient structure in disordered proteins depending on polymer concentration.

PubMed

Rusinga, Farai I; Weis, David D

2017-08-01

The effects of macromolecular crowding on the transient structure of intrinsically disordered proteins is not well-understood. Crowding by biological molecules inside cells could modulate transient structure and alter IDP function. Volume exclusion theory and observations of structured proteins suggest that IDP transient structure would be stabilized by macromolecular crowding. Amide hydrogen exchange (HX) of IDPs in highly concentrated polymer solutions would provide valuable insights into IDP transient structure under crowded conditions. Here, we have used mass spectrometry to measure HX by a transiently helical random coil domain of the activator of thyroid and retinoid receptor (ACTR) in solutions containing 300 g L -1 and 400 g L -1 of Ficoll, a synthetic polysaccharide, using a recently-developed strong cation exchange-based cleanup method [Rusinga, et al., Anal Chem 2017;89:1275-1282]. Transiently helical regions of ACTR exchanged faster in 300 g L -1 Ficoll than in dilute buffer. In contrast, one transient helix exchanged more slowly in 400 g L -1 Ficoll. Nonspecific interactions destabilize ACTR helicity in 300 g L -1 Ficoll because ACTR engages with the Ficoll polymer mesh. In contrast, 400 g L -1 Ficoll is a semi-dilute solution where ACTR cannot engage the Ficoll mesh. At this higher concentration, volume exclusion stabilizes ACTR helicity because ACTR is compacted in interstitial spaces between Ficoll molecules. Our results suggest that the interplay between nonspecific interactions and volume exclusion in different cellular compartments could modulate IDP function by altering the stability of IDP transient structures. Proteins 2017; 85:1468-1479. © 2017 Wiley Periodicals, Inc. © 2017 Wiley Periodicals, Inc.

Automated thin-film analyses of anhydrous interplanetary dust particles in the analytical electron microscope

NASA Technical Reports Server (NTRS)

Bradley, J. P.; Germani, M. S.; Brownlee, D. E.

1989-01-01

An AEM apparatus equipped with digital beam control has obtained quantitative point-count analyses of thin sections taken from eight anhydrous chondritic interplanetary dust particles (IDPs); between 200 and 500 X-ray analyses were collected from each thin section and analyzed for Mg, Al, Si, S, Ca, Cr, Mn, Fe, and Ni. Two types of anhydrous chondritic aggregates were observed in the eight IDPs: one highly porous, the other less so. The eight anhydrous IDPs are characterizable as mixtures of fine- and coarse-grained aggregates, large mineral grains, glass, and carbonaceous materials. Their elemental concentrations follow those of solar abundances, suggesting that they are unperturbed by aqueous alteration.

Entropic Elasticity in the Giant Muscle Protein Titin

NASA Astrophysics Data System (ADS)

Morgan, Ian; Saleh, Omar

Intrinsically disordered proteins (IDPs) are a large and functionally important class of proteins that lack a fixed three-dimensional structure. Instead, they adopt a conformational ensemble of states which facilitates their biological function as molecular linkers, springs, and switches. Due to their conformational flexibility, it can be difficult to study IDPs using typical experimental methods. To overcome this challenge, we use a high-resolution single-molecule magnetic stretching technique to quantify IDP flexibility. We apply this technique to the giant muscle protein titin, measuring its elastic response at low forces. We present results demonstrating that titin's native elastic response derives from the combined entropic elasticity of its ordered and disordered domains.

Trace Element Abundance Measurements on Cosmic Dust Particles

NASA Technical Reports Server (NTRS)

Flynn, George

1996-01-01

The X-Ray Microprobe on beamline X-26A at the National Synchrotron Light Source (NSLS) at Brookhaven National Laboratory was used to determine the abundances of elements from Cr through Sr in individual interplanetary dust particles (IDPs) collected from the Earth's stratosphere and the Scanning Transmission X-ray Microscope (STXM) on beamline X-1A at the NSLS was used to determine the carbon abundances and spatial distributions in IDPs. In addition, modeling was performed in an attempt to associate particular types of IDPs with specific types of parent bodies, and thus to infer the chemistry, mineralogy, and structural properties of those parent bodies.

Cloud-based Web Services for Near-Real-Time Web access to NPP Satellite Imagery and other Data

NASA Astrophysics Data System (ADS)

Evans, J. D.; Valente, E. G.

2010-12-01

We are building a scalable, cloud computing-based infrastructure for Web access to near-real-time data products synthesized from the U.S. National Polar-Orbiting Environmental Satellite System (NPOESS) Preparatory Project (NPP) and other geospatial and meteorological data. Given recent and ongoing changes in the the NPP and NPOESS programs (now Joint Polar Satellite System), the need for timely delivery of NPP data is urgent. We propose an alternative to a traditional, centralized ground segment, using distributed Direct Broadcast facilities linked to industry-standard Web services by a streamlined processing chain running in a scalable cloud computing environment. Our processing chain, currently implemented on Amazon.com's Elastic Compute Cloud (EC2), retrieves raw data from NASA's Moderate Resolution Imaging Spectroradiometer (MODIS) and synthesizes data products such as Sea-Surface Temperature, Vegetation Indices, etc. The cloud computing approach lets us grow and shrink computing resources to meet large and rapid fluctuations (twice daily) in both end-user demand and data availability from polar-orbiting sensors. Early prototypes have delivered various data products to end-users with latencies between 6 and 32 minutes. We have begun to replicate machine instances in the cloud, so as to reduce latency and maintain near-real time data access regardless of increased data input rates or user demand -- all at quite moderate monthly costs. Our service-based approach (in which users invoke software processes on a Web-accessible server) facilitates access into datasets of arbitrary size and resolution, and allows users to request and receive tailored and composite (e.g., false-color multiband) products on demand. To facilitate broad impact and adoption of our technology, we have emphasized open, industry-standard software interfaces and open source software. Through our work, we envision the widespread establishment of similar, derived, or interoperable systems for

The "Interaction with Disabled Persons" Scale: Revisiting Its Internal Consistency and Factor Structure, and Examining Item-Level Properties

ERIC Educational Resources Information Center

Iacono, Teresa; Tracy, Jane; Keating, Jenny; Brown, Ted

2009-01-01

The "Interaction with Disabled Persons" scale (IDP) has been used in research into baseline attitudes and to evaluate whether a shift in attitudes towards people with developmental disabilities has occurred following some form of intervention. This research has been conducted on the assumption that the IDP measures attitudes as a…

Overview of the Ocean Observer Satellite Study

NASA Astrophysics Data System (ADS)

Cunningham, J. D.; McGuire, J. P.; Pichel, W. G.; Gerber, A. J.

2002-12-01

A two-year study of ocean satellite remote sensing requirements and instrument/satellite options is nearing completion. This Ocean Observer Study was sponsored by the U.S. Dept. of Commerce/Dept. of Defense/National Aeronautics and Space Administration Integrated Program Office, whose mission is to develop the future U.S. National Polar-Orbiting Operational Environmental Satellite System (NPOESS). A comprehensive Ocean Observer User Requirements Document has been drafted by a team of over 150 government, academic, and private sector scientists, engineers, and administrators. Included are requirements for open and coastal ocean surface, cryospheric, hydrologic, and some land/hazard and atmospheric boundary layer parameters. This document was then used as input to the instrument and satellite study (conducted by the Jet Propulsion Laboratory) which produced five different instrument/satellite configuration options designed to address the maximum number of requirements which will not be met with the already-approved NPOESS instruments. Instruments studied include a synthetic aperture radar (SAR), an altimeter, and a hyper-spectral coastal infrared/visible imager. After analyzing the alternatives, it appears that one of the best options is a two-satellite system consisting of (1) an altimeter mission in the Topex/Poseidon orbit carrying both wide-swath and delayed doppler altimeters, and (2) a multi-polarization, multi-frequency, multi-mode interferometric SAR mission including a coastal imager in a polar sun-synchronous orbit. This paper summarizes the user requirements process, briefly describes the notional satellite configuration, and presents some of the capabilities of the instruments.

Dynamical Coupling of Intrinsically Disordered Proteins and Their Hydration Water: Comparison with Folded Soluble and Membrane Proteins

PubMed Central

Gallat, F.-X.; Laganowsky, A.; Wood, K.; Gabel, F.; van Eijck, L.; Wuttke, J.; Moulin, M.; Härtlein, M.; Eisenberg, D.; Colletier, J.-P.; Zaccai, G.; Weik, M.

2012-01-01

Hydration water is vital for various macromolecular biological activities, such as specific ligand recognition, enzyme activity, response to receptor binding, and energy transduction. Without hydration water, proteins would not fold correctly and would lack the conformational flexibility that animates their three-dimensional structures. Motions in globular, soluble proteins are thought to be governed to a certain extent by hydration-water dynamics, yet it is not known whether this relationship holds true for other protein classes in general and whether, in turn, the structural nature of a protein also influences water motions. Here, we provide insight into the coupling between hydration-water dynamics and atomic motions in intrinsically disordered proteins (IDP), a largely unexplored class of proteins that, in contrast to folded proteins, lack a well-defined three-dimensional structure. We investigated the human IDP tau, which is involved in the pathogenic processes accompanying Alzheimer disease. Combining neutron scattering and protein perdeuteration, we found similar atomic mean-square displacements over a large temperature range for the tau protein and its hydration water, indicating intimate coupling between them. This is in contrast to the behavior of folded proteins of similar molecular weight, such as the globular, soluble maltose-binding protein and the membrane protein bacteriorhodopsin, which display moderate to weak coupling, respectively. The extracted mean square displacements also reveal a greater motional flexibility of IDP compared with globular, folded proteins and more restricted water motions on the IDP surface. The results provide evidence that protein and hydration-water motions mutually affect and shape each other, and that there is a gradient of coupling across different protein classes that may play a functional role in macromolecular activity in a cellular context. PMID:22828339

Insights into the Molecular Mechanisms of Alzheimer’s and Parkinson’s Diseases with Molecular Simulations: Understanding the Roles of Artificial and Pathological Missense Mutations in Intrinsically Disordered Proteins Related to Pathology

PubMed Central

Coskuner-Weber, Orkid

2018-01-01

Amyloid-β and α-synuclein are intrinsically disordered proteins (IDPs), which are at the center of Alzheimer’s and Parkinson’s disease pathologies, respectively. These IDPs are extremely flexible and do not adopt stable structures. Furthermore, both amyloid-β and α-synuclein can form toxic oligomers, amyloid fibrils and other type of aggregates in Alzheimer’s and Parkinson’s diseases. Experimentalists face challenges in investigating the structures and thermodynamic properties of these IDPs in their monomeric and oligomeric forms due to the rapid conformational changes, fast aggregation processes and strong solvent effects. Classical molecular dynamics simulations complement experiments and provide structural information at the atomic level with dynamics without facing the same experimental limitations. Artificial missense mutations are employed experimentally and computationally for providing insights into the structure-function relationships of amyloid-β and α-synuclein in relation to the pathologies of Alzheimer’s and Parkinson’s diseases. Furthermore, there are several natural genetic variations that play a role in the pathogenesis of familial cases of Alzheimer’s and Parkinson’s diseases, which are related to specific genetic defects inherited in dominant or recessive patterns. The present review summarizes the current understanding of monomeric and oligomeric forms of amyloid-β and α-synuclein, as well as the impacts of artificial and pathological missense mutations on the structural ensembles of these IDPs using molecular dynamics simulations. We also emphasize the recent investigations on residual secondary structure formation in dynamic conformational ensembles of amyloid-β and α-synuclein, such as β-structure linked to the oligomerization and fibrillation mechanisms related to the pathologies of Alzheimer’s and Parkinson’s diseases. This information represents an important foundation for the successful and efficient drug

A late Miocene dust shower from the break-up of an asteroid in the main belt.

PubMed

Farley, Kenneth A; Vokrouhlický, David; Bottke, William F; Nesvorný, David

2006-01-19

Throughout the history of the Solar System, Earth has been bombarded by interplanetary dust particles (IDPs), which are asteroid and comet fragments of diameter approximately 1-1,000 microm. The IDP flux is believed to be in quasi-steady state: particles created by episodic main belt collisions or cometary fragmentation replace those removed by comminution, dynamical ejection, and planetary or solar impact. Because IDPs are rich in 3He, seafloor sediment 3He concentrations provide a unique means of probing the major events that have affected the IDP flux and its source bodies over geological timescales. Here we report that collisional disruption of the >150-km-diameter asteroid that created the Veritas family 8.3 +/- 0.5 Myr ago also produced a transient increase in the flux of interplanetary dust-derived 3He. The increase began at 8.2 +/- 0.1 Myr ago, reached a maximum of approximately 4 times pre-event levels, and dissipated over approximately 1.5 Myr. The terrestrial IDP accretion rate was overwhelmingly dominated by Veritas family fragments during the late Miocene. No other event of this magnitude over the past approximately 10(8) yr has been deduced from main belt asteroid orbits. One remarkably similar event is present in the 3He record 35 Myr ago, but its origin by comet shower or asteroid collision remains uncertain.

Microcrystals and Amorphous Material in Comets and Primitive Meteorites: Keys to Understanding Processes in the Early Solar System

NASA Technical Reports Server (NTRS)

Nuth, J. A.; Brearley, A. J.; Scott, E. R. D.

2004-01-01

Comets, fine-grained matrices of chondrites, and chondritic interplanetary dust particles (IDPs) are each composed of both crystalline and amorphous silicates. The primitive solar nebula, in which comets and asteroids accreted, was formed from the collapsed core of a Giant Molecular Cloud, that, in turn, condensed from materials present in the interstellar medium (ISM). Despite observations that reveal the presence of crystalline magnesium silicate minerals in the shells of very high mass-loss-rate stars [1,2], typical silicate grains in the ISM are most likely to be amorphous, given their relatively long residence time in such a high radiation environment. An upper limit of 3% crystalline grains can be derived from their non-detection in spectra of ISM solids [3]. If the vast majority of grains that enter the primitive solar nebula are amorphous, then the observation of crystalline dust in comets and primitive chondrite matrices indicates the action of specific processes required to transform the amorphous starting materials into the crystals that are observed.

Outreach to internally displaced persons in Bogotá, Colombia: challenges and potential solutions

PubMed Central

Shultz, James M; García, Natalia Muñoz; Ceballos, Ángela Milena Gómez; Florez, Luis Jorge Hernandez; Araya, Ricardo; Verdeli, Helen; Espinel, Zelde; Bolivar, Sandra Patricia Cipagauta; Neria, Yuval

2014-01-01

Programs that provide services for internally displaced persons (IDPs) in Colombia, South America face challenges when attempting to engage and enroll the target population of forced migrants they intend to serve. Innovative multi-strategy outreach approaches must be used in order to effectively seek, recruit, provide services, monitor, and retain IDPs. PMID:28229001

Norwegian Field Research Projects in Industrial Democracy.

ERIC Educational Resources Information Center

Qvale, Thoralf Ulrik

The Industrial Democracy Programme (IDP) of Norway seeks to create conditions for a self-supporting change process in industry, rather than implementing a ready-made model, believing that it is more valuable for employees to learn to change their organization than for the specific solution to be found. The central problems, therefore, have been to…

Physically-Retrieving Cloud and Thermodynamic Parameters from Ultraspectral IR Measurements

NASA Technical Reports Server (NTRS)

Zhou, Daniel K.; Smith, William L., Sr.; Liu, Xu; Larar, Allen M.; Mango, Stephen A.; Huang, Hung-Lung

2007-01-01

A physical inversion scheme has been developed, dealing with cloudy as well as cloud-free radiance observed with ultraspectral infrared sounders, to simultaneously retrieve surface, atmospheric thermodynamic, and cloud microphysical parameters. A fast radiative transfer model, which applies to the clouded atmosphere, is used for atmospheric profile and cloud parameter retrieval. A one-dimensional (1-d) variational multi-variable inversion solution is used to improve an iterative background state defined by an eigenvector-regression-retrieval. The solution is iterated in order to account for non-linearity in the 1-d variational solution. It is shown that relatively accurate temperature and moisture retrievals can be achieved below optically thin clouds. For optically thick clouds, accurate temperature and moisture profiles down to cloud top level are obtained. For both optically thin and thick cloud situations, the cloud top height can be retrieved with relatively high accuracy (i.e., error < 1 km). NPOESS Airborne Sounder Testbed Interferometer (NAST-I) retrievals from the Atlantic-THORPEX Regional Campaign are compared with coincident observations obtained from dropsondes and the nadir-pointing Cloud Physics Lidar (CPL). This work was motivated by the need to obtain solutions for atmospheric soundings from infrared radiances observed for every individual field of view, regardless of cloud cover, from future ultraspectral geostationary satellite sounding instruments, such as the Geosynchronous Imaging Fourier Transform Spectrometer (GIFTS) and the Hyperspectral Environmental Suite (HES). However, this retrieval approach can also be applied to the ultraspectral sounding instruments to fly on Polar satellites, such as the Infrared Atmospheric Sounding Interferometer (IASI) on the European MetOp satellite, the Cross-track Infrared Sounder (CrIS) on the NPOESS Preparatory Project and the following NPOESS series of satellites.

Mental health problems among internally displaced persons in Darfur.

PubMed

Hamid, Abdalla A R M; Musa, Saif A

2010-08-01

War victims are regarded as one of the highest risk groups for mental disturbances. This study investigated the effects of the Darfur conflict on mental health of 430 internally displaced persons (IDPs) from three camps located around Fasher and Nyala towns. A stratified random sampling technique was used to select participants. Male participants represented 50.6% of the sample while female participants represented 49.4%. The Posttraumatic Stress Disorder Checklist and the General Health Questionnaire (GHQ-28) were used in addition to a questionnaire measuring demographic variables and living conditions. It was hypothesized that high prevalence of posttraumatic stress disorder (PTSD) symptoms and of nonpsychotic psychiatric symptoms will be evident. Results showed a high dissatisfaction rate (72%) with living conditions among IDPs. There was also high prevalence of PTSD (54%) and general distress (70%) among IDPs. Female participants showed more somatic symptoms than their male counterparts. Married participants were more distressed, anxious, and showed more social dysfunction, while single ones reported more avoidance symptoms. Significant differences related to date of displacement were found in PTSD and hyperarousal. The group of IDPs displaced in 2003 scored higher on these scales than those displaced in 2004 and 2005. There was also significant difference related to date of displacement in distress, somatic symptoms, depression, anxiety, and social dysfunction. IDPs displaced in 2003 scored higher on these scales. Results are discussed in light of the study hypotheses and previous findings. It is concluded that three factors might affect the dissatisfaction of IDPs with living conditions inside camps. These are: lack of employment, unsuitability of food items, and lack of security around camps. It was recommended that psychological support services should be among the prime relief services provided by aid agencies.

Order-disorder transition of intrinsically disordered kinase inducible transactivation domain of CREB

NASA Astrophysics Data System (ADS)

Liu, Hao; Guo, Xiang; Han, Jingcheng; Luo, Ray; Chen, Hai-Feng

2018-06-01

Transcription factor cyclic Adenosine monophosphate response-element binding protein plays a critical role in the cyclic AMP response pathway via its intrinsically disordered kinase inducible transactivation domain (KID). KID is one of the most studied intrinsically disordered proteins (IDPs), although most previous studies focus on characterizing its disordered state structures. An interesting question that remains to be answered is how the order-disorder transition occurs at experimental conditions. Thanks to the newly developed IDP-specific force field ff14IDPSFF, the quality of conformer sampling for IDPs has been dramatically improved. In this study, molecular dynamics (MD) simulations were used to study the order-to-disorder transition kinetics of KID based on the good agreement with the experiment on its disordered-state properties. Specifically, we tested four force fields, ff99SBildn, ff99IDPs, ff14IDPSFF, and ff14IDPs in the simulations of KID and found that ff14IDPSFF can generate more diversified disordered conformers and also reproduce more accurate experimental secondary chemical shifts. Kinetics analysis of MD simulations demonstrates that the order-disorder transition of KID obeys the first-order kinetics, and the transition nucleus is I127/L128/L141. The possible transition pathways from the nucleus to the last folded residues were identified as I127-R125-L138-L141-S143-A145 and L128-R125-L138-L141-S143-A145 based on a residue-level dynamical network analysis. These computational studies not only provide testable prediction/hypothesis on the order-disorder transition of KID but also confirm that the ff14IDPSFF force field can be used to explore the correlation between the structure and function of IDPs.

Insight into Primordial Solar System Oxygen Reservoirs from Returned Cometary Samples

NASA Technical Reports Server (NTRS)

Brownlee, D. E.; Messenger, S.

2004-01-01

The recent successful rendezvous of the Stardust spacecraft with comet Wild-2 will be followed by its return of cometary dust to Earth in January 2006. Results from two separate dust impact detectors suggest that the spacecraft collected approximately the nominal fluence of at least 1,000 particles larger than 15 micrometers in size. While constituting only about one microgram total, these samples will be sufficient to answer many outstanding questions about the nature of cometary materials. More than two decades of laboratory studies of stratospherically collected interplanetary dust particles (IDPs) of similar size have established the necessary microparticle handling and analytical techniques necessary to study them. It is likely that some IDPs are in fact derived from comets, although complex orbital histories of individual particles have made these assignments difficult to prove. Analysis of bona fide cometary samples will be essential for answering some fundamental outstanding questions in cosmochemistry, such as (1) the proportion of interstellar and processed materials that comprise comets and (2) whether the Solar System had a O-16-rich reservoir. Abundant silicate stardust grains have recently been discovered in anhydrous IDPs, in far greater abundances (200 5,500 ppm) than those in meteorites (25 ppm). Insight into the more subtle O isotopic variations among chondrites and refractory phases will require significantly higher precision isotopic measurements on micrometer-sized samples than are currently available.

Bridge over troubled proline: assignment of intrinsically disordered proteins using (HCA)CON(CAN)H and (HCA)N(CA)CO(N)H experiments concomitantly with HNCO and i(HCA)CO(CA)NH.

PubMed

Hellman, Maarit; Piirainen, Henni; Jaakola, Veli-Pekka; Permi, Perttu

2014-01-01

NMR spectroscopy is by far the most versatile and information rich technique to study intrinsically disordered proteins (IDPs). While NMR is able to offer residue level information on structure and dynamics, assignment of chemical shift resonances in IDPs is not a straightforward process. Consequently, numerous pulse sequences and assignment protocols have been developed during past several years, targeted especially for the assignment of IDPs, including experiments that employ H(N), H(α) or (13)C detection combined with two to six indirectly detected dimensions. Here we propose two new HN-detection based pulse sequences, (HCA)CON(CAN)H and (HCA)N(CA)CO(N)H, that provide correlations with (1)H(N)(i - 1), (13)C'(i - 1) and (15)N(i), and (1)H(N)(i + 1), (13)C'(i) and (15)N(i) frequencies, respectively. Most importantly, they offer sequential links across the proline bridges and enable filling the single proline gaps during the assignment. We show that the novel experiments can efficiently complement the information available from existing HNCO and intraresidual i(HCA)CO(CA)NH pulse sequences and their concomitant usage enabled >95 % assignment of backbone resonances in cytoplasmic tail of adenosine receptor A2A in comparison to 73 % complete assignment using the HNCO/i(HCA)CO(CA)NH data alone.

Ultrafine-grained mineralogy and matrix chemistry of olivine-rich chondritic interplanetary dust particles

NASA Technical Reports Server (NTRS)

Rietmeijer, F. J. M.

1989-01-01

Olivine-rich chondritic interplanetary dust particles (IDPs) are an important subset of fluffy chondritic IDPs collected in the earth's stratosphere. Particles in this subset are characterized by a matrix of nonporous, ultrafine-grained granular units. Euhedral single crystals, crystals fragments, and platey single crystals occur dispersed in the matrix. Analytical electron microscopy of granular units reveals predominant magnesium-rich olivines and FeNi-sulfides embedded in amorphous carbonaceous matrix material. The variable ratio of ultrafine-grained minerals vs. carbonaceous matrix material in granular units support variable C/Si ratios, and some fraction of sulfur is associated with carbonaceous matrix material. The high Mg/(Mg+Fe) ratios in granular units is similar to this distribution in P/Comet Halley dust. The chondritic composition of fine-grained, polycrystalline IDPs gradually breaks down into nonchondritic, and ultimately, single mineral compositions as a function of decreased particle mass. The relationship between particle mass and composition in the matrix of olivine-rich chondritic IDPs is comparable with the relationship inferred for P/Comet Halley dust.

Consensus on updating immunizations in patients with primary immunodeficiencies

PubMed

2018-04-01

Las inmunodeficiencias primarias (IDP) constituyen un grupo de enfermedades hereditarias que afectan el número y/o la función de los distintos componentes del sistema inmune. Su prevalencia es de 1:1000-2000 nacimientos. Comprenden defectos de la inmunidad adaptativa, defectos de la inmunidad innata, inmunodeficiencias con fenotipos característicos, trastornos de la regulación inmune, síndromes autoinflamatorios, defectos de los fagocitos y del sistema del complemento y defectos considerados fenocopias de IDP. La vacunación con vacunas inactivadas es segura y puede ser efectiva en muchas inmunodeficiencias; las vacunas vivas atenuadas pueden no ser protectoras en ciertas IDP o presentarse como enfermedad vacunal asociada a la inmunización, lo que conlleva una alta morbimortalidad. Con el objetivo de actualizar las recomendaciones de vacunas en pacientes con IDP, el Comité Nacional de Infectología y el Grupo de Trabajo de Inmunología trabajaron sobre las vacunas que podían indicarse a estos pacientes, convivientes y el equipo de salud.

Skin diseases among internally displaced Tawerghans living in camps in Benghazi, Libya.

PubMed

Elfaituri, Safa S

2016-09-01

Benghazi has received many internally displaced persons (IDPs) from other Libyan cities as a result of the armed conflict in Libya. These groups have significant health problems associated with their displacement, including skin diseases. This study aimed to determine the spectrum and frequency of skin diseases among people living in IDP camps in Benghazi. A total of 480 IDP camp residents with complaints of skin diseases were studied over a period of 6 months. All subjects were ethnic Tawerghans; about three-quarters were female and half were adults. The disease types found to occur at the highest frequencies were skin infections (40.0%), followed by xerosis (31.3%), eczema (18.3%), acne (17.0%), hair-related diseases (6.7%), and psychosomatic diseases (3.0%). People who are resident in IDP camps have skin problems similar to those of other populations in similar circumstances. They have increased vulnerability to infections, environment-associated disorders such as xerosis cutis and eczema, and diseases of psychosomatic origin. © 2015 The International Society of Dermatology.

The thermal history of interplanetary dust particles collected in the Earth's stratosphere

NASA Technical Reports Server (NTRS)

Nier, A. O.; Schlutter, D. J.

1993-01-01

Fragments of 24 individual interplanetary dust particles (IDPs) collected in the Earth's stratosphere were obtained from NASA's Johnson Space Center collection and subjected to pulse-heating sequences to extract He and Ne and to learn about the thermal history of the particles. A motivation for the investigation was to see if the procedure would help distinguish between IDPs of asteroidal and cometary origin. The use of a sequence of short-duration heat pulses to perform the extractions is an improvement over the employment of a step-heating sequence, as was used in a previous investigation. The particles studied were fragments of larger parent IDPs, other fragments of which, in coordinated experiments, are undergoing studies of elemental and mineralogical composition in other laboratories. While the present investigation will provide useful temperature history data for the particles, the relatively large size of the parent IDPs (approximately 40 micrometers in diameter) resulted in high entry deceleration temperatures. This limited the usefulness of the study for distinguishing between particles of asteroidal and cometary origin.

Training and deployment of lay refugee/internally displaced persons to provide basic health services in camps: a systematic review

PubMed Central

Ehiri, John E.; Gunn, Jayleen K.L.; Center, Katherine E.; Li, Ying; Rouhani, Mae; Ezeanolue, Echezona E.

2014-01-01

Background Training of lay refugees/internally displaced persons (IDPs) and deploying them to provide basic health services to other women, children, and families in camps is perceived to be associated with public health benefits. However, there is limited evidence to support this hypothesis. Objectives To assess the effects of interventions to train and deploy lay refugees and/or IDPs for the provision of basic health service to other women, children, and families in camps. Methods PubMed, Science and Social Science Citation Indices, PsycINFO, EMBASE, POPLINE, CINAHL, and reference lists of relevant articles were searched (from inception to June 30, 2014) with the aim of identifying studies that reported the effects of interventions that trained and deployed lay refugees and/or IDPs for the provision of basic health service to other women, children, and families in camps. Two investigators independently reviewed all titles and abstracts to identify potentially relevant articles. Discrepancies were resolved by repeated review, discussion, and consensus. Study quality assessment was undertaken using standard protocols. Results Ten studies (five cross-sectional, four pre-post, and one post-test only) conducted in Africa (Guinea and Tanzania), Central America (Belize), and Asia (Myanmar) were included. The studies demonstrated some positive impact on population health associated with training and deployment of trained lay refugees/IDPs as health workers in camps. Reported effects included increased service coverage, increased knowledge about disease symptoms and prevention, increased adoption of improved treatment seeking and protective behaviors, increased uptake of services, and improved access to reproductive health information. One study, which assessed the effect of peer refugee health education on sexual and reproductive health, did not demonstrate a marked reduction in unintended pregnancies among refugee/IDP women. Conclusion Although available evidence

Si Isotopes of Brownleeite

NASA Technical Reports Server (NTRS)

Nakamura-Messenger, K.; Messenger, Scott R.; Ito, M.; Keller, L. P.; Clemett, S. J.; Jones, J. H.; Tatsuoka, H.; Zolensky, M. E.; Tatsuoka, H.

2010-01-01

Brownleeite is a manganese silicide, ideally stoichiometric MnSi, not previously observed in nature until its discovery within an interplanetary dust particle (IDP) that likely originated from a comet [1]. Three discrete brownleeite grains in the IDP L2055 I3 (4 microns in size, hereafter IDP I3) were identified with maximum dimensions of 100, 250 and 600 nm and fully analyzed using scanning-transmission electron microscopy (STEM) [1]. One of the grains (100 nm in size) was poikilitically enclosed by low-Fe, Mn-enriched (LIME) olivine. LIME olivine is epitaxial to the brownleeite with the brownleeite (200) parallel to the olivine c* [1]. LIME olivine is an enigmatic phase first reported from chondritic porous IDPs and some unequilibrated ordinary chondrites [ 2], that is commonly observed in chondritic-porous IDPs. Recently, LIME olivine has been also found in comet Wild-2 (Stardust) samples [3], indicating that LIME olivine is a common mineral component of comets. LIME olivine has been proposed to form as a high temperature condensate in the protosolar nebula [2]. Brownleeite grains also likely formed as high-temperature condensates either in the early Solar System or in the outflow of an evolved star or supernova explosion [1]. The isotopic composition of the brownleeite grains may strongly constrain their ultimate source. To test this hypothesis, we performed isotopic analyses of the brownleeite and the associated LIME olivine, using the NASA/JSC NanoSIMS 50L ion microprobe.

A decade and a half of protein intrinsic disorder: Biology still waits for physics

PubMed Central

Uversky, Vladimir N

2013-01-01

The abundant existence of proteins and regions that possess specific functions without being uniquely folded into unique 3D structures has become accepted by a significant number of protein scientists. Sequences of these intrinsically disordered proteins (IDPs) and IDP regions (IDPRs) are characterized by a number of specific features, such as low overall hydrophobicity and high net charge which makes these proteins predictable. IDPs/IDPRs possess large hydrodynamic volumes, low contents of ordered secondary structure, and are characterized by high structural heterogeneity. They are very flexible, but some may undergo disorder to order transitions in the presence of natural ligands. The degree of these structural rearrangements varies over a very wide range. IDPs/IDPRs are tightly controlled under the normal conditions and have numerous specific functions that complement functions of ordered proteins and domains. When lacking proper control, they have multiple roles in pathogenesis of various human diseases. Gaining structural and functional information about these proteins is a challenge, since they do not typically “freeze” while their “pictures are taken.” However, despite or perhaps because of the experimental challenges, these fuzzy objects with fuzzy structures and fuzzy functions are among the most interesting targets for modern protein research. This review briefly summarizes some of the recent advances in this exciting field and considers some of the basic lessons learned from the analysis of physics, chemistry, and biology of IDPs. PMID:23553817

Small Particles Intact Capture Experiment (SPICE)

NASA Technical Reports Server (NTRS)

Nishioka, Ken-Ji; Carle, G. C.; Bunch, T. E.; Mendez, David J.; Ryder, J. T.

1994-01-01

The Small Particles Intact Capture Experiment (SPICE) will develop technologies and engineering techniques necessary to capture nearly intact, uncontaminated cosmic and interplanetary dust particles (IDP's). Successful capture of such particles will benefit the exobiology and planetary science communities by providing particulate samples that may have survived unaltered since the formation of the solar system. Characterization of these particles may contribute fundamental data to our knowledge of how these particles could have formed into our planet Earth and, perhaps, contributed to the beginnings of life. The term 'uncontaminated' means that captured cosmic and IDP particles are free of organic contamination from the capture process and the term 'nearly intact capture' means that their chemical and elemental components are not materially altered during capture. The key to capturing cosmic and IDP particles that are organic-contamination free and nearly intact is the capture medium. Initial screening of capture media included organic foams, multiple thin foil layers, and aerogel (a silica gel); but, with the exception of aerogel, the requirements of no contamination or nearly intact capture were not met. To ensure no contamination of particles in the capture process, high-purity aerogel was chosen. High-purity aerogel results in high clarity (visual clearness), a useful quality in detection and recovery of embedded captured particles from the aerogel. P. Tsou at the Jet Propulsion Laboratory (JPL) originally described the use of aerogel for this purpose and reported laboratory test results. He has flown aerogel as a 'GAS-can Lid' payload on STS-47 and is evaluating the results. The Timeband Capture Cell Experiment (TICCE), a Eureca 1 experiment, is also flying aerogel and is scheduled for recovery in late April.

Trace element content of chondritic cosmic dust: Volatile enrichments, thermal alterations, and the possibility of contamination

NASA Technical Reports Server (NTRS)

Flynn, G. J.; Sutton, S. R.; Bajt, S.

1993-01-01

Trace element abundances in 51 chondritic Interplanetary Dust Particles (IDP's) were measured by Synchrotron X-Ray Fluorescence (SXRF). The data allow us to determine an average composition of chondritic IDP's and to examine the questions of volatile loss during the heating pulse experienced on atmospheric entry and possible element addition due to contamination during atmospheric entry, stratospheric residence, and curation.

Invention Development Program Helps Nurture NCI at Frederick Technologies | Poster

Cancer.gov

The Invention Development Fund (IDF) was piloted by the Technology Transfer Center (TTC) in 2014 to facilitate the commercial development of NCI technologies. The IDF received a second round of funding from the NCI Office of the Director and the Office of Budget and Management to establish the Invention Development Program (IDP) for fiscal year 2016. The IDP is using these

Synchrotron FTIR Examination of Interplanetary Dust Particles: An Effort to Determine the Compounds and Minerals in Interstellar and Circumstellar Dust

NASA Technical Reports Server (NTRS)

Flynn, G. J.; Keller, L. P.

2002-01-01

Some interplanetary dust particles (IDPs), collected by NASA from the Earth's stratosphere, are the most primitive extraterrestrial material available for laboratory analysis. Many exhibit isotopic anomalies in H, N, and O, suggesting they contain preserved interstellar matter. We report the preliminary results of a comparison of the infrared absorption spectra of subunits of the IDPs with astronomical spectra of interstellar grains.

The alphabet of intrinsic disorder

PubMed Central

Uversky, Vladimir N

2013-01-01

The ability of a protein to fold into unique functional state or to stay intrinsically disordered is encoded in its amino acid sequence. Both ordered and intrinsically disordered proteins (IDPs) are natural polypeptides that use the same arsenal of 20 proteinogenic amino acid residues as their major building blocks. The exceptional structural plasticity of IDPs, their capability to exist as heterogeneous structural ensembles and their wide array of important disorder-based biological functions that complements functional repertoire of ordered proteins are all rooted within the peculiar differential usage of these building blocks by ordered proteins and IDPs. In fact, some residues (so-called disorder-promoting residues) are noticeably more common in IDPs than in sequences of ordered proteins, which, in their turn, are enriched in several order-promoting residues. Furthermore, residues can be arranged according to their “disorder promoting potencies,” which are evaluated based on the relative abundances of various amino acids in ordered and disordered proteins. This review continues a series of publications on the roles of different amino acids in defining the phenomenon of protein intrinsic disorder and concerns glutamic acid, which is the second most disorder-promoting residue. PMID:28516010

Are Charge-State Distributions a Reliable Tool Describing Molecular Ensembles of Intrinsically Disordered Proteins by Native MS?

NASA Astrophysics Data System (ADS)

Natalello, Antonino; Santambrogio, Carlo; Grandori, Rita

2017-01-01

Native mass spectrometry (MS) has become a central tool of structural proteomics, but its applicability to the peculiar class of intrinsically disordered proteins (IDPs) is still object of debate. IDPs lack an ordered tridimensional structure and are characterized by high conformational plasticity. Since they represent valuable targets for cancer and neurodegeneration research, there is an urgent need of methodological advances for description of the conformational ensembles populated by these proteins in solution. However, structural rearrangements during electrospray-ionization (ESI) or after the transfer to the gas phase could affect data obtained by native ESI-MS. In particular, charge-state distributions (CSDs) are affected by protein conformation inside ESI droplets, while ion mobility (IM) reflects protein conformation in the gas phase. This review focuses on the available evidence relating IDP solution ensembles with CSDs, trying to summarize cases of apparent consistency or discrepancy. The protein-specificity of ionization patterns and their responses to ligands and buffer conditions suggests that CSDs are imprinted to protein structural features also in the case of IDPs. Nevertheless, it seems that these proteins are more easily affected by electrospray conditions, leading in some cases to rearrangements of the conformational ensembles.

Post-entry and volcanic contaminant abundances of zinc, copper, selenium, germanium and gallium in stratospheric micrometeorites

NASA Technical Reports Server (NTRS)

Rietmeijer, Frans J. M.

1995-01-01

Some fraction of Zn, Cu, Se, Ga and Ge in chondritic interplanetary dust particles (IDPs) collected in the lower stratosphere between 1981 May and 1984 June has a volcanic origin. I present a method to evaluate the extent of this unavoidable type of stratospheric contamination for individual particles. The mass-normalized abundances for Cu and Ge as a function of mass-normalized stratospheric residence time show their time-integrated stratospheric aerosol abundances. The Zn, Se and Ga abundances show a subdivision into two groups that span approximately two-year periods following the eruptions of the Mount St. Helens (1980 May) and El Chichon (1982 April) volcanoes. Elemental abundances in particles collected at the end of each two-year period indicate low, but not necessarily ambient, volcanic stratospheric abundances. Using this time-integrated baseline, I calculate the straospheric contaminant fractions in nine IDPs and show that Zn, SE and Ga abundances in chondritic IDPs derive in part from stratospheric aerosol contaminants. Post-entry elemental abundances (i.e., the amount that survived atmospheric entry heating of the IDP) show enrichments relative to the CI abundances but in a smaller number of particles than previously suggested.

Thermodynamic contribution of backbone conformational entropy in the binding between SH3 domain and proline-rich motif.

PubMed

Zeng, Danyun; Shen, Qingliang; Cho, Jae-Hyun

2017-02-26

Biological functions of intrinsically disordered proteins (IDPs), and proteins containing intrinsically disordered regions (IDRs) are often mediated by short linear motifs, like proline-rich motifs (PRMs). Upon binding to their target proteins, IDPs undergo a disorder-to-order transition which is accompanied by a large conformational entropy penalty. Hence, the molecular mechanisms underlying control of conformational entropy are critical for understanding the binding affinity and selectivity of IDPs-mediated protein-protein interactions (PPIs). Here, we investigated the backbone conformational entropy change accompanied by binding of the N-terminal SH3 domain (nSH3) of CrkII and PRM derived from guanine nucleotide exchange factor 1 (C3G). In particular, we focused on the estimation of conformational entropy change of disordered PRM upon binding to the nSH3 domain. Quantitative characterization of conformational dynamics of disordered peptides like PRMs is limited. Hence, we combined various methods, including NMR model-free analysis, δ2D, DynaMine, and structure-based calculation of entropy loss. This study demonstrates that the contribution of backbone conformational entropy change is significant in the PPIs mediated by IDPs/IDRs. Copyright © 2017 Elsevier Inc. All rights reserved.

IDPT: Insights into potential intrinsically disordered proteins through transcriptomic analysis of genes for prostate carcinoma epigenetic data.

PubMed

Mallik, Saurav; Sen, Sagnik; Maulik, Ujjwal

2016-07-15

Involvement of intrinsically disordered proteins (IDPs) with various dreadful diseases like cancer is an interesting research topic. In order to gain novel insights into the regulation of IDPs, in this article, we perform a transcriptomic analysis of mRNAs (genes) for transcripts encoding IDPs on a human multi-omics prostate carcinoma dataset having both gene expression and methylation data. In this regard, firstly the genes that consist of both the expression and methylation data, and that are corresponding to the cancer-related prostate-tissue-specific disordered proteins of MobiDb database, are selected. We apply standard t-test for determining differentially expressed genes as well as differentially methylated genes. A network having these genes and their targeter miRNAs from Diana Tarbase v7.0 database and corresponding Transcription Factors from TRANSFAC and ITFP databases, is then built. Thereafter, we perform literature search, and KEGG pathway and Gene Ontology analyses using DAVID database. Finally, we report several significant potential gene-markers (with the corresponding IDPs) that have inverse relationship between differential expression and methylation patterns, and that are hub genes of the TF-miRNA-gene network. Copyright © 2016 Elsevier B.V. All rights reserved.

Are Charge-State Distributions a Reliable Tool Describing Molecular Ensembles of Intrinsically Disordered Proteins by Native MS?

PubMed

Natalello, Antonino; Santambrogio, Carlo; Grandori, Rita

2017-01-01

Native mass spectrometry (MS) has become a central tool of structural proteomics, but its applicability to the peculiar class of intrinsically disordered proteins (IDPs) is still object of debate. IDPs lack an ordered tridimensional structure and are characterized by high conformational plasticity. Since they represent valuable targets for cancer and neurodegeneration research, there is an urgent need of methodological advances for description of the conformational ensembles populated by these proteins in solution. However, structural rearrangements during electrospray-ionization (ESI) or after the transfer to the gas phase could affect data obtained by native ESI-MS. In particular, charge-state distributions (CSDs) are affected by protein conformation inside ESI droplets, while ion mobility (IM) reflects protein conformation in the gas phase. This review focuses on the available evidence relating IDP solution ensembles with CSDs, trying to summarize cases of apparent consistency or discrepancy. The protein-specificity of ionization patterns and their responses to ligands and buffer conditions suggests that CSDs are imprinted to protein structural features also in the case of IDPs. Nevertheless, it seems that these proteins are more easily affected by electrospray conditions, leading in some cases to rearrangements of the conformational ensembles. Graphical Abstract ᅟ.

Merging Ocean Color Data From Multiple Missions. Chapter 6

NASA Technical Reports Server (NTRS)

Gregg, Watson W.

2003-01-01

Oceanic phytoplankton may play an important role in the cycling of carbon on the Earth, through the uptake of carbon dioxide in the process of photosynthesis. Although they are ubiquitous in the global oceans, their abundances and dynamics are difficult to estimate, primarily due to the vast spatial extent of the oceans and the short time scales over which their abundances can change. Consequently, the effects of oceanic phytoplankton on biogeochemical cycling, climate change, and fisheries are not well known. In response to the potential importance of phytoplankton in the global carbon cycle and the lack of comprehensive data, NASA and the international community have established high priority satellite missions designed to acquire and produce high quality ocean color data (Table 6.1). Ten of the missions are routine global observational missions: the Ocean Color and Temperature Sensor (OCTS), the Polarization and Directionality of the Earth's Reflectances sensor (POLDER), Sea-viewing Wide Field-of-view Sensor (SeaWiFS), Moderate Resolution Imaging Spectrometer-AM (MODIS-AM), Medium Resolution Imaging Spectrometer (MERIS), Global Imager (GLI), MODIS-PM, Super-GLI (S-GLI), and the Visible/Infrared Imager and Radiometer Suite (VIIRS) on the NPOESS Preparatory Project (NPP) and the National Polar-orbiting Operational Environmental Satellite System (NPOESS). In addition, there are several other missions capable of providing ocean color data on smaller scales. Most of these missions contain the spectral band complement considered necessary to derive oceanic chlorophyll concentrations and other related parameters. Many contain additional bands that can provide important ancillary information about the optical and biological state of the oceans.

Highly accurate FTIR observations from the scanning HIS aircraft instrument

NASA Astrophysics Data System (ADS)

Revercomb, Henry E.; Tobin, David C.; Knuteson, Robert O.; Best, Fred A.; Smith, William L., Sr.; van Delst, Paul F. W.; LaPorte, Daniel D.; Ellington, Scott D.; Werner, Mark W.; Dedecker, Ralph G.; Garcia, Raymond K.; Ciganovich, Nick N.; Howell, Hugh B.; Olson, Erik R.; Dutcher, Steven B.; Taylor, Joseph K.

2005-01-01

Development in the mid 80s of the High-resolution Interferometer Sounder (HIS) instrument for the high altitude NASA ER2 aircraft demonstrated the capability for advanced atmospheric temperature and water vapor sounding and set the stage for new satellite instruments that are now becoming a reality [AIRS(2002), CrIS(2006), IASI(2006), GIFTS(200?), HES(2013)]. Follow-on developments at the University of Wisconsin that employ Fourier Transform Infrared (FTIR) for Earth observations include the ground-based Atmospheric Emitted Radiance Interferometer (AERI) and the new Scanning HIS aircraft instrument. The Scanning HIS is a smaller version of the original HIS that uses cross-track scanning to enhance spatial coverage. Scanning HIS and its close cousin, the NPOESS Airborne Sounder Testbed (NAST), are being used for satellite instrument validation and for atmospheric research. A novel detector configuration on Scanning HIS allows the incorporation of a single focal plane and cooler with three or four spectral bands that view the same spot on the ground. The calibration accuracy of the S-HIS and results from recent field campaigns are presented, including validation comparisons with the NASA EOS infrared observations (AIRS and MODIS). Aircraft comparisons of this type provide a mechanism for periodically testing the absolute calibration of spacecraft instruments with instrumentation for which the calibration can be carefully maintained on the ground. This capability is especially valuable for assuring the long-term consistency and accuracy of climate observations, including those from the NASA EOS spacecrafts (Terra, Aqua and Aura) and the new complement of NPOESS operational instruments. It is expected that aircraft flights of the S-HIS and the NAST will be used to check the long-term stability of AIRS and the NPOESS operational follow-on sounder, the Cross-track Infrared Sounder (CrIS), over the life of the mission.

NPOESS, Essential Climates Variables and Climate Change

NASA Astrophysics Data System (ADS)

Forsythe-Newell, S. P.; Bates, J. J.; Barkstrom, B. R.; Privette, J. L.; Kearns, E. J.

2008-12-01

Advancement in understanding, predicting and mitigating against climate change implies collaboration, close monitoring of Essential Climate Variable (ECV)s through development of Climate Data Record (CDR)s and effective action with specific thematic focus on human and environmental impacts. Towards this end, NCDC's Scientific Data Stewardship (SDS) Program Office developed Climate Long-term Information and Observation system (CLIO) for satellite data identification, characterization and use interrogation. This "proof-of-concept" online tool provides the ability to visualize global CDR information gaps and overlaps with options to temporally zoom-in from satellite instruments to climate products, data sets, data set versions and files. CLIO provides an intuitive one-stop web site that displays past, current and planned launches of environmental satellites in conjunction with associated imagery and detailed information. This tool is also capable of accepting and displaying Web-based input from Subject Matter Expert (SME)s providing a global to sub-regional scale perspective of all ECV's and their impacts upon climate studies. SME's can access and interact with temporal data from the past and present, or for future planning of products, datasets/dataset versions, instruments, platforms and networks. CLIO offers quantifiable prioritization of ECV/CDR impacts that effectively deal with climate change issues, their associated impacts upon climate, and this offers an intuitively objective collaboration and consensus building tool. NCDC's latest tool empowers decision makers and the scientific community to rapidly identify weaknesses and strengths in climate change monitoring strategies and significantly enhances climate change collaboration and awareness.

OMPS Sensor Performance and Algorithm Description

NASA Astrophysics Data System (ADS)

Branham, M. S.; Farrow, S. V.; Novicki, M.; Bhaswar, S.; Baker, B.

2009-12-01

The Ozone Mapping and Profiler Suite (OMPS), built by Ball Aerospace, is the next-generation U.S. ozone monitoring sensor suite, designed and built for the National Polar-orbiting Operational Environmental Satellite System (NPOESS), under contract to the Integrated Program Office, administered by the Air Force, National Oceanic and Atmospheric Administration (NOAA), and National Aeronautics and Space Administration (NASA) under contract to Northrop Grumman. The first flight of an OMPS is scheduled for early 2011 on the NPOESS Preparatory Project (NPP) satellite. The OMPS sensor data will be used to generate the ozone calibrated sensor data and environmental data record (EDR) products. The final OMPS sensor performance and algorithms for NPP will be presented, now that the FM1 flight sensor suite has completed sell off and is integrated on the NPP spacecraft. Challenges requiring future development, and during intensive calibration/validation on orbit will be described. Also, an overview of the sensor suite, the FM1 measurement performance, and details of the retrieval algorithms will be provided in this presentation.

High Vertically Resolved Atmospheric State Revealed with IASI Single FOV Retrievals under All-weather Conditions

NASA Technical Reports Server (NTRS)

Zhou, Daniel K.; Liu, Xu; Larar, Allen M.; Smith, William L.; Taylor, Jonathan P.; Schluessel, L. Peter; Strow, Larrybee; Mango, Stephen A.

2008-01-01

The Infrared Atmospheric Sounding Interferometer (IASI) on the MetOp satellite was launched on October 19, 2006. The Joint Airborne IASI Validation Experiment (JAIVEx) was conducted during April 2007 mainly for validation of the IASI on the MetOp satellite. IASI possesses an ultra-spectral resolution of 0.25 cm(exp -1) and a spectral coverage from 645 to 2760 cm(exp -1). Ultra-spectral resolution infrared spectral radiance obtained from near nadir observations provide atmospheric, surface, and cloud property information. An advanced retrieval algorithm with a fast radiative transfer model, including cloud effects, is used for atmospheric profile and cloud parameter retrieval. Preliminary retrievals of atmospheric soundings, surface properties, and cloud optical/microphysical properties with the IASI observations are obtained and presented. These retrievals are further inter-compared with those obtained from airborne FTS system, such as the NPOESS Airborne Sounder Testbed - Interferometer (NAST-I), dedicated dropsondes, radiosondes, and ground based Raman Lidar. The capabilities of satellite ultra-spectral sounder such as the IASI are investigated to benefit future NPOESS operation.

Sampling Long- versus Short-Range Interactions Defines the Ability of Force Fields To Reproduce the Dynamics of Intrinsically Disordered Proteins.

PubMed

Mercadante, Davide; Wagner, Johannes A; Aramburu, Iker V; Lemke, Edward A; Gräter, Frauke

2017-09-12

Molecular dynamics (MD) simulations have valuably complemented experiments describing the dynamics of intrinsically disordered proteins (IDPs), particularly since the proposal of models to solve the artificial collapse of IDPs in silico. Such models suggest redefining nonbonded interactions, by either increasing water dispersion forces or adopting the Kirkwood-Buff force field. These approaches yield extended conformers that better comply with experiments, but it is unclear if they all sample the same intrachain dynamics of IDPs. We have tested this by employing MD simulations and single-molecule Förster resonance energy transfer spectroscopy to sample the dimensions of systems with different sequence compositions, namely strong and weak polyelectrolytes. For strong polyelectrolytes in which charge effects dominate, all the proposed solutions equally reproduce the expected ensemble's dimensions. For weak polyelectrolytes, at lower cutoffs, force fields abnormally alter intrachain dynamics, overestimating excluded volume over chain flexibility or reporting no difference between the dynamics of different chains. The TIP4PD water model alone can reproduce experimentally observed changes in extensions (dimensions), but not quantitatively and with only weak statistical significance. Force field limitations are reversed with increased interaction cutoffs, showing that chain dynamics are critically defined by the presence of long-range interactions. Force field analysis aside, our study provides the first insights into how long-range interactions critically define IDP dimensions and raises the question of which length range is crucial to correctly sample the overall dimensions and internal dynamics of the large group of weakly charged yet highly polar IDPs.

Atomistic details of the disordered states of KID and pKID. Implications in coupled binding and folding.

PubMed

Ganguly, Debabani; Chen, Jianhan

2009-04-15

Intrinsically disordered proteins (IDPs) are a newly recognized class of functional proteins for which a lack of stable tertiary fold is required for function. Because of the heterogeneous and dynamical nature, molecular modeling is necessary to provide the missing details of disordered states of IDP that are crucial for understanding their functions. In particular, generalized Born (GB) implicit solvent, combined with replica exchange (REX), might offer an optimal balance between accuracy and efficiency for modeling IDPs. We carried out extensive REX simulations in an optimized GB force field to characterize the disordered states of a regulatory IDP, KID domain of transcription factor CREB, and its phosphorylated form, pKID. The results revealed that both KID and pKID, though highly disordered on the tertiary level, are compact and mainly occupy a small number of helical substates. Interestingly, although phosphorylation of KID Ser133 leads only to marginal changes in average helicities on the ensemble level, underlying conformational substates differ significantly. In particular, pSer133 appears to restrict the accessible conformational space of the loop region and thus reduces the entropic cost of KID folding upon binding to the KIX domain of CREB-binding protein. Such an expanded role of phosphorylation in the KID:KIX recognition was not previously recognized because of a lack of substantial conformational changes on the ensemble level and inaccessibility of the structural details from experiments. The results also suggest that an implicit solvent-based modeling framework, despite various existing limitations, might be feasible for accurate atomistic simulation of small IDPs in general.

Binding Mechanisms of Intrinsically Disordered Proteins: Theory, Simulation, and Experiment

PubMed Central

Mollica, Luca; Bessa, Luiza M.; Hanoulle, Xavier; Jensen, Malene Ringkjøbing; Blackledge, Martin; Schneider, Robert

2016-01-01

In recent years, protein science has been revolutionized by the discovery of intrinsically disordered proteins (IDPs). In contrast to the classical paradigm that a given protein sequence corresponds to a defined structure and an associated function, we now know that proteins can be functional in the absence of a stable three-dimensional structure. In many cases, disordered proteins or protein regions become structured, at least locally, upon interacting with their physiological partners. Many, sometimes conflicting, hypotheses have been put forward regarding the interaction mechanisms of IDPs and the potential advantages of disorder for protein-protein interactions. Whether disorder may increase, as proposed, e.g., in the “fly-casting” hypothesis, or decrease binding rates, increase or decrease binding specificity, or what role pre-formed structure might play in interactions involving IDPs (conformational selection vs. induced fit), are subjects of intense debate. Experimentally, these questions remain difficult to address. Here, we review experimental studies of binding mechanisms of IDPs using NMR spectroscopy and transient kinetic techniques, as well as the underlying theoretical concepts and numerical methods that can be applied to describe these interactions at the atomic level. The available literature suggests that the kinetic and thermodynamic parameters characterizing interactions involving IDPs can vary widely and that there may be no single common mechanism that can explain the different binding modes observed experimentally. Rather, disordered proteins appear to make combined use of features such as pre-formed structure and flexibility, depending on the individual system and the functional context. PMID:27668217

In vitro biomechanical comparison after fixed- and mobile-core artificial cervical disc replacement versus fusion

PubMed Central

Lou, Jigang; Li, Yuanchao; Wang, Beiyu; Meng, Yang; Wu, Tingkui; Liu, Hao

2017-01-01

Abstract In vitro biomechanical analysis after cervical disc replacement (CDR) with a novel artificial disc prosthesis (mobile core) was conducted and compared with the intact model, simulated fusion, and CDR with a fixed-core prosthesis. The purpose of this experimental study was to analyze the biomechanical changes after CDR with a novel prosthesis and the differences between fixed- and mobile-core prostheses. Six human cadaveric C2–C7 specimens were biomechanically tested sequentially in 4 different spinal models: intact specimens, simulated fusion, CDR with a fixed-core prosthesis (Discover, DePuy), and CDR with a mobile-core prosthesis (Pretic-I, Trauson). Moments up to 2 Nm with a 75 N follower load were applied in flexion–extension, left and right lateral bending, and left and right axial rotation. The total range of motion (ROM), segmental ROM, and adjacent intradiscal pressure (IDP) were calculated and analyzed in 4 different spinal models, as well as the differences between 2 disc prostheses. Compared with the intact specimens, the total ROM, segmental ROM, and IDP at the adjacent segments showed no significant difference after arthroplasty. Moreover, CDR with a mobile-core prosthesis presented a little higher values of target segment (C5/6) and total ROM than CDR with a fixed-core prosthesis (P > .05). Besides, the difference in IDP at C4/5 after CDR with 2 prostheses was without statistical significance in all the directions of motion. However, the IDP at C6/7 after CDR with a mobile-core prosthesis was lower than CDR with a fixed-core prosthesis in flexion, extension, and lateral bending, with significant difference (P < .05), but not under axial rotation. CDR with a novel prosthesis was effective to maintain the ROM at the target segment and did not affect the ROM and IDP at the adjacent segments. Moreover, CDR with a mobile-core prosthesis presented a little higher values of target segment and total ROM, but lower IDP at the inferior

Establishment of an immortalized mouse dermal papilla cell strain with optimized culture strategy.

PubMed

Guo, Haiying; Xing, Yizhan; Zhang, Yiming; He, Long; Deng, Fang; Ma, Xiaogen; Li, Yuhong

2018-01-01

Dermal papilla (DP) plays important roles in hair follicle regeneration. Long-term culture of mouse DP cells can provide enough cells for research and application of DP cells. We optimized the culture strategy for DP cells from three dimensions: stepwise dissection, collagen I coating, and optimized culture medium. Based on the optimized culture strategy, we immortalized primary DP cells with SV40 large T antigen, and established several immortalized DP cell strains. By comparing molecular expression and morphologic characteristics with primary DP cells, we found one cell strain named iDP6 was similar with primary DP cells. Further identifications illustrate that iDP6 expresses FGF7 and α-SMA, and has activity of alkaline phosphatase. During the process of characterization of immortalized DP cell strains, we also found that cells in DP were heterogeneous. We successfully optimized culture strategy for DP cells, and established an immortalized DP cell strain suitable for research and application of DP cells.

Establishment of an immortalized mouse dermal papilla cell strain with optimized culture strategy

PubMed Central

Zhang, Yiming; He, Long; Deng, Fang; Ma, Xiaogen

2018-01-01

Dermal papilla (DP) plays important roles in hair follicle regeneration. Long-term culture of mouse DP cells can provide enough cells for research and application of DP cells. We optimized the culture strategy for DP cells from three dimensions: stepwise dissection, collagen I coating, and optimized culture medium. Based on the optimized culture strategy, we immortalized primary DP cells with SV40 large T antigen, and established several immortalized DP cell strains. By comparing molecular expression and morphologic characteristics with primary DP cells, we found one cell strain named iDP6 was similar with primary DP cells. Further identifications illustrate that iDP6 expresses FGF7 and α-SMA, and has activity of alkaline phosphatase. During the process of characterization of immortalized DP cell strains, we also found that cells in DP were heterogeneous. We successfully optimized culture strategy for DP cells, and established an immortalized DP cell strain suitable for research and application of DP cells. PMID:29383288

Dynamic protein interaction networks and new structural paradigms in signaling

PubMed Central

Csizmok, Veronika; Follis, Ariele Viacava; Kriwacki, Richard W.; Forman-Kay, Julie D.

2017-01-01

Understanding signaling and other complex biological processes requires elucidating the critical roles of intrinsically disordered proteins and regions (IDPs/IDRs), which represent ~30% of the proteome and enable unique regulatory mechanisms. In this review we describe the structural heterogeneity of disordered proteins that underpins these mechanisms and the latest progress in obtaining structural descriptions of ensembles of disordered proteins that are needed for linking structure and dynamics to function. We describe the diverse interactions of IDPs that can have unusual characteristics such as “ultrasensitivity” and “regulated folding and unfolding”. We also summarize the mounting data showing that large-scale assembly and protein phase separation occurs within a variety of signaling complexes and cellular structures. In addition, we discuss efforts to therapeutically target disordered proteins with small molecules. Overall, we interpret the remodeling of disordered state ensembles due to binding and post-translational modifications within an expanded framework for allostery that provides significant insights into how disordered proteins transmit biological information. PMID:26922996

Abundant Solar Nebula Solids in Comets

NASA Technical Reports Server (NTRS)

Messenger, S.; Keller, L. P.; Nakamura-Messenger, K.; Nguyen, A. N.; Clemett, S.

2016-01-01

Comets have been proposed to consist of unprocessed interstellar materials together with a variable amount of thermally annealed interstellar grains. Recent studies of cometary solids in the laboratory have shown that comets instead consist of a wide range of materials from across the protoplanetary disk, in addition to a minor complement of interstellar materials. These advances were made possible by the return of direct samples of comet 81P/Wild 2 coma dust by the NASA Stardust mission and recent advances in microscale analytical techniques. Isotopic studies of 'cometary' chondritic porous interplanetary dust particles (CP-IDPs) and comet 81P/Wild 2 Stardust samples show that preserved interstellar materials are more abundant in comets than in any class of meteorite. Identified interstellar materials include sub-micron-sized presolar silicates, oxides, and SiC dust grains and some fraction of the organic material that binds the samples together. Presolar grain abundances reach 1 weight percentage in the most stardust-rich CP-IDPs, 50 times greater than in meteorites. Yet, order of magnitude variations in presolar grain abundances among CP-IDPs suggest cometary solids experienced significant variations in the degree of processing in the solar nebula. Comets contain a surprisingly high abundance of nebular solids formed or altered at high temperatures. Comet 81P/Wild 2 samples include 10-40 micron-sized, refractory Ca- Al-rich inclusion (CAI)-, chondrule-, and ameboid olivine aggregate (AOA)-like materials. The O isotopic compositions of these refractory materials are remarkably similar to their meteoritic counterparts, ranging from 5 percent enrichments in (sup 16) O to near-terrestrial values. Comet 81P/Wild 2 and CP-IDPs also contain abundant Mg-Fe crystalline and amorphous silicates whose O isotopic compositions are also consistent with Solar System origins. Unlike meteorites, that are dominated by locally-produced materials, comets appear to be composed of

Calibration of Crustal Historical Earthquakes from Intra-Carpathian Region of Romania

NASA Astrophysics Data System (ADS)

Oros, Eugen; Popa, Mihaela; Rogozea, Maria

2017-12-01

the process of estimating the quality and credibility of the primary data used (e.g. IDPs, isoseismals) and in the correct determination of Mw.

Origins of GEMS Grains

NASA Technical Reports Server (NTRS)

Messenger, S.; Walker, R. M.

2012-01-01

Interplanetary dust particles (IDPs) collected in the Earth s stratosphere contain high abundances of submicrometer amorphous silicates known as GEMS grains. From their birth as condensates in the outflows of oxygen-rich evolved stars, processing in interstellar space, and incorporation into disks around new stars, amorphous silicates predominate in most astrophysical environments. Amorphous silicates were a major building block of our Solar System and are prominent in infrared spectra of comets. Anhydrous interplanetary dust particles (IDPs) thought to derive from comets contain abundant amorphous silicates known as GEMS (glass with embedded metal and sulfides) grains. GEMS grains have been proposed to be isotopically and chemically homogenized interstellar amorphous silicate dust. We evaluated this hypothesis through coordinated chemical and isotopic analyses of GEMS grains in a suite of IDPs to constrain their origins. GEMS grains show order of magnitude variations in Mg, Fe, Ca, and S abundances. GEMS grains do not match the average element abundances inferred for ISM dust containing on average, too little Mg, Fe, and Ca, and too much S. GEMS grains have complementary compositions to the crystalline components in IDPs suggesting that they formed from the same reservoir. We did not observe any unequivocal microstructural or chemical evidence that GEMS grains experienced prolonged exposure to radiation. We identified four GEMS grains having O isotopic compositions that point to origins in red giant branch or asymptotic giant branch stars and supernovae. Based on their O isotopic compositions, we estimate that 1-6% of GEMS grains are surviving circumstellar grains. The remaining 94-99% of GEMS grains have O isotopic compositions that are indistinguishable from terrestrial materials and carbonaceous chondrites. These isotopically solar GEMS grains either formed in the Solar System or were completely homogenized in the interstellar medium (ISM). However, the

Invention Development Program Helps Nurture NCI at Frederick Technologies | Poster

Cancer.gov

The Invention Development Fund (IDF) was piloted by the Technology Transfer Center (TTC) in 2014 to facilitate the commercial development of NCI technologies. The IDF received a second round of funding from the NCI Office of the Director and the Office of Budget and Management to establish the Invention Development Program (IDP) for fiscal year 2016. The IDP is using these funds to help advance a second set of inventions.

The JPSS Ground Project Algorithm Verification, Test and Evaluation System

NASA Astrophysics Data System (ADS)

Vicente, G. A.; Jain, P.; Chander, G.; Nguyen, V. T.; Dixon, V.

2016-12-01

The Government Resource for Algorithm Verification, Independent Test, and Evaluation (GRAVITE) is an operational system that provides services to the Suomi National Polar-orbiting Partnership (S-NPP) Mission. It is also a unique environment for Calibration/Validation (Cal/Val) and Data Quality Assessment (DQA) of the Join Polar Satellite System (JPSS) mission data products. GRAVITE provides a fast and direct access to the data and products created by the Interface Data Processing Segment (IDPS), the NASA/NOAA operational system that converts Raw Data Records (RDR's) generated by sensors on the S-NPP into calibrated geo-located Sensor Data Records (SDR's) and generates Mission Unique Products (MUPS). It also facilitates algorithm investigation, integration, checkouts and tuning, instrument and product calibration and data quality support, monitoring and data/products distribution. GRAVITE is the portal for the latest S-NPP and JPSS baselined Processing Coefficient Tables (PCT's) and Look-Up-Tables (LUT's) and hosts a number DQA offline tools that takes advantage of the proximity to the near-real time data flows. It also contains a set of automated and ad-hoc Cal/Val tools used for algorithm analysis and updates, including an instance of the IDPS called GRAVITE Algorithm Development Area (G-ADA), that has the latest installation of the IDPS algorithms running in an identical software and hardware platforms. Two other important GRAVITE component are the Investigator-led Processing System (IPS) and the Investigator Computing Facility (ICF). The IPS is a dedicated environment where authorized users run automated scripts called Product Generation Executables (PGE's) to support Cal/Val and data quality assurance offline. This data-rich and data-driven service holds its own distribution system and allows operators to retrieve science data products. The ICF is a workspace where users can share computing applications and resources and have full access to libraries and

The 8-13 micron spectra of comets and the composition of silicate grains

NASA Technical Reports Server (NTRS)

Hanner, Martha S.; Lynch, David K.; Russell, Ray W.

1994-01-01

We have analyzed the existing spectra of seven comets which show an emission feature at 7.8-13 micrometers. Most have been converted to a common calibration, taking into account the SiO feature in late-type standard stars. The spectra are compared with spectra of the Trapezium, interplanetary dust particles (IDPs), laboratory mineral samples, and small particle emission models. The emission spectra show a variety of shapes; there is no unique 'cometary silicate'. A peak at 11.20-11.25 micrometers, indicative of small crystalline olivine particles, is seen in only three comets of this sample, P/Halley, Bradfield 1987 XXIX, and Levy 1990 XX. The widths of the emission features range from 2.6 to 4.1 micrometers (FWHM). To explain the differing widths and the broad 9.8 micrometers maximum, glassy silicate particles, including both pyroxene and olivine compositions, are the most plausible candidates. Calculations of emission models confirm that small grains of glassy silicate well mixed with carbonaceous material are plausible cometary constituents. No single class of chondritic aggregate IDPs exhibits spectra closely matching the comet spectra. A mixture of IDP spectra, particularly the glass-rich aggregates, approximately matches the spectra of comets P/Halley, Levy, and Bradfield 1987 XXIX. Yet, if comets are simply a mix of IDP types, it is puzzling that the classes of IDPs are so distinct. None of the comet spectra match the spectrum of the Trapezium. Thus, the mineralogy of the cometary silicates is not the same as that of the interstellar medium. The presence of a component of crystalline silicates in comets may be evidence of mixing between high- and low-temperature regions in the solar nebula.

The Impact of O-Glycan Chemistry on the Stability of Intrinsically Disordered Proteins

DOE Office of Scientific and Technical Information (OSTI.GOV)

Beckham, Gregg T; Prates, Erica T; Crowley, Michael F

2018-03-02

Protein glycosylation is a diverse post-translational modification that serves myriad biological functions. O-linked glycans in particular vary widely in extent and chemistry in eukaryotes, with secreted proteins from fungi and yeast commonly exhibiting O-mannosylation in intrinsically disordered regions of proteins, likely for proteolysis protection, among other functions. However, it is not well understood why mannose is often the preferred glycan, and more generally, if the neighboring protein sequence and glycan have coevolved to protect against proteolysis in glycosylated intrinsically disordered proteins (IDPs). Here, we synthesized variants of a model IDP, specifically a natively O-mannosylated linker from a fungal enzyme, withmore » a-O-linked mannose, glucose, and galactose moieties, along with a non-glycosylated linker. Upon exposure to thermolysin, O-mannosylation, by far, provides the highest extent of proteolysis protection. To explain this observation, extensive molecular dynamics simulations were conducted, revealing that the axial configuration of the C2-hydroxyl group (2-OH) of a-mannose adjacent to the glycan-peptide bond strongly influences the conformational features of the linker. Specifically, a-mannose restricts the torsions of the IDP main chain more than other glycans whose equatorial 2-OH groups exhibit interactions that favor perpendicular glycan-protein backbone orientation. We suggest that IDP stiffening due to O-mannosylation impairs protease action, with contributions from protein-glycan interactions, protein flexibility, and protein stability. Our results further imply that resistance to proteolysis is an important driving force for evolutionary selection of a-mannose in eukaryotic IDPs, and more broadly, that glycan motifs for proteolysis protection likely coevolve with the protein sequence to which they attach.« less

Validity and reliability of Arabic version of the ID Pain screening questionnaire in the assessment of neuropathic pain.

PubMed

Abu-Shaheen, Amani; Yousef, Shehu; Riaz, Muhammad; Nofal, Abdullah; Khan, Sarfaraz; Heena, Humariya

2018-01-01

Diagnosis of neuropathic pain (NP) can be challenging. The ID Pain (ID-P) questionnaire, a screening tool for NP, has been used widely both in the original version and translated forms. The aim of this study was to develop an Arabic version of ID-P and assess its validity and reliability in detecting neuropathic pain. The original ID-P was translated in Arabic language and administered to the study population. Reliability of the Arabic version was evaluated by percentage observed agreement, and Cohen's kappa; and validity by sensitivity, specificity, correctly classified, and receiver operating characteristic (ROC) curve. Physician diagnosis was considered as the gold standard for comparing the diagnostic accuracy. The study included 375 adult patients (153 [40.8%] with NP; 222 [59.2%] with nociceptive pain). Overall observed percentage agreement and Cohen's kappa were >90% and >0.80, respectively. Median (range) score of ID-P scale was 3 (2-4) and 1 (0-2) in the NP group and NocP group, respectively (p<0.001). Area under the ROC curve was 0.808 (95% CI, 0.764-0.851). For the cut-off value of ≥2, sensitivity was 84.3%, specificity was 66.7%, and correct classification was 73.9%. Thus, the Arabic version of ID-P showed moderate reliability and validity as a pain assessment tool. This article presents the psychometric properties of the Arabic version of ID Pain questionnaire. This Arabic version may serve as a simple yet important screening tool, and help in appropriate management of neuropathic pain, specifically in primary care centers in the Kingdom of Saudi Arabia.

Should we excise? Are there any clinical or histologic features that predict upgrade in papillomas, incidental or non-incidental?

PubMed

Zaleski, Michael; Chen, Yun An; Chetlen, Alison L; Mack, Julie; Xu, Liyan; Dodge, Daleela G; Karamchandani, Dipti M

2018-05-11

The clinical decision to excise intraductal papilloma (IDP) without atypia diagnosed on biopsy remains controversial. We sought to establish clinical and histologic predictors (if any) which may predict upgrade in IDP. 296 biopsies (in 278 women) with histologic diagnosis of IDP without atypia were retrospectively identified and placed into Incidental (no corresponding imaging correlate), or Non-incidental (positive imaging correlate) groups. 253/296 (85.5%) cases were non-incidental, and 43/296 (14.5%) were incidental. 73.1% (185/253) non-incidental and 48.8% (21/43) incidental cases underwent excision. 12.4% (23/185) non-incidental cases underwent an upgrade to cancer or high-risk lesion; namely 8-Ductal carcinoma in situ (DCIS), 8-atypical ductal hyperplasia (ADH), 6-lobular neoplasia, and 1-flat epithelial atypia. There was no histopathologic feature on the biopsy in the non-incidental group which predicted upgrade; however a past history of atypia was significantly associated with upgrade. 2 of the 21 incidental cases upgraded (1 to ADH and 1 to lobular neoplasia); the former had a past history of ADH. Both incidental upgrades were >1 mm in size, and were not completely excised on the biopsy. None of the incidental cases which appeared completely excised on biopsy upgraded, irrespective of the size on biopsy. These findings suggest that all non-incidental IDPs should be considered candidates for surgical excision, given the 12.4% upgrade rate and no definitive histologic predictors of upgrade. Patients with incidental IDPs (if <1 mm, completely excised on biopsy and with no history of high risk breast lesion) can be spared excision. Copyright © 2018 Elsevier Inc. All rights reserved.

Topology-based modeling of intrinsically disordered proteins: balancing intrinsic folding and intermolecular interactions.

PubMed

Ganguly, Debabani; Chen, Jianhan

2011-04-01

Coupled binding and folding is frequently involved in specific recognition of so-called intrinsically disordered proteins (IDPs), a newly recognized class of proteins that rely on a lack of stable tertiary fold for function. Here, we exploit topology-based Gō-like modeling as an effective tool for the mechanism of IDP recognition within the theoretical framework of minimally frustrated energy landscape. Importantly, substantial differences exist between IDPs and globular proteins in both amino acid sequence and binding interface characteristics. We demonstrate that established Gō-like models designed for folded proteins tend to over-estimate the level of residual structures in unbound IDPs, whereas under-estimating the strength of intermolecular interactions. Such systematic biases have important consequences in the predicted mechanism of interaction. A strategy is proposed to recalibrate topology-derived models to balance intrinsic folding propensities and intermolecular interactions, based on experimental knowledge of the overall residual structure level and binding affinity. Applied to pKID/KIX, the calibrated Gō-like model predicts a dominant multistep sequential pathway for binding-induced folding of pKID that is initiated by KIX binding via the C-terminus in disordered conformations, followed by binding and folding of the rest of C-terminal helix and finally the N-terminal helix. This novel mechanism is consistent with key observations derived from a recent NMR titration and relaxation dispersion study and provides a molecular-level interpretation of kinetic rates derived from dispersion curve analysis. These case studies provide important insight into the applicability and potential pitfalls of topology-based modeling for studying IDP folding and interaction in general. Copyright © 2011 Wiley-Liss, Inc.

Social phobia among long-term internally displaced persons: An epidemiological study of adults in two internally displaced person settlements in Sudan.

PubMed

Salah, Tarig Taha Mohamed; Ayazi, Touraj; Lien, Lars; Eide, Arne; Hauff, Edvard

2015-09-01

Despite the high worldwide prevalence and association with other mental disorders and disability, only few studies are available on social phobia in low-income countries. This study aims to assess the prevalence, socio-demographic correlates and comorbidity of social phobia and its association with disability among long-term internally displaced persons (IDPs) in one urban and one rural area in Central Sudan. This cross-sectional study was implemented in two IDP areas in Central Sudan. Data were collected during face-to-face interviews using structured questionnaires to assess socio-demographic factors and the Mini International Neuropsychiatric Interview (MINI) to determine psychiatric diagnoses. A total of 1,876 adults were enrolled from both study areas. The overall lifetime prevalence of social phobia was 14.2%, with higher rates among IDPs in the rural area and among those who were less educated. Social phobia was associated with other mental disorders in both study areas. Disability and prolonged displacement increased the risk of having social phobia in the rural area. Further work needs to be done to improve our understanding and to establish proper interventions in dealing with social phobia, other common mental disorders and disability among long-term IDPs in these impoverished areas. © The Author(s) 2014.

The transition state structure for binding between TAZ1 of CBP and the disordered Hif-1α CAD.

PubMed

Lindström, Ida; Andersson, Eva; Dogan, Jakob

2018-05-18

Intrinsically disordered proteins (IDPs) are common in eukaryotes. However, relatively few experimental studies have addressed the nature of the rate-limiting transition state for the coupled binding and folding reactions involving IDPs. By using site-directed mutagenesis in combination with kinetics measurements we have here characterized the transition state for binding between the globular TAZ1 domain of CREB binding protein and the intrinsically disordered C-terminal activation domain of Hif-1α (Hif-1α CAD). A total of 17 Hif-1α CAD point-mutations were generated and a Φ-value binding analysis was carried out. We found that native hydrophobic binding interactions are not formed at the transition state. We also investigated the effect the biologically important Hif-1α CAD Asn-803 hydroxylation has on the binding kinetics, and found that the whole destabilization effect due the hydroxylation is within the dissociation rate constant. Thus, the rate-limiting transition state is "disordered-like", with native hydrophobic binding contacts being formed cooperatively after the rate-limiting barrier, which is clearly shown by linear free energy relationships. The same behavior was observed in a previously characterized TAZ1/IDP interaction, which may suggest common features for the rate-limiting transition state for TAZ1/IDP interactions.

The Effects of Physiological Biomechanical Loading on Intradiscal Pressure and Annulus Stress in Lumbar Spine: A Finite Element Analysis

PubMed Central

Zahari, Siti Nurfaezah; Rahim, Nor Raihanah Abdull; Kamarul, Tunku

2017-01-01

The present study was conducted to examine the effects of body weight on intradiscal pressure (IDP) and annulus stress of intervertebral discs at lumbar spine. Three-dimensional finite element model of osseoligamentous lumbar spine was developed subjected to follower load of 500 N, 800 N, and 1200 N which represent the loads for individuals who are normal and overweight with the pure moments at 7.5 Nm in flexion and extension motions. It was observed that the maximum IDP was 1.26 MPa at L1-L2 vertebral segment. However, the highest increment of IDP was found at L4-L5 segment where the IDP was increased to 30% in flexion and it was more severe at extension motion reaching to 80%. Furthermore, the maximum annulus stress also occurred at the L1-L2 segment with 3.9 MPa in extension motion. However, the highest increment was also found at L4-L5 where the annulus stress increased to 17% in extension motion. Based on these results, the increase of physiological loading could be an important factor to the increment of intradiscal pressure and annulus fibrosis stress at all intervertebral discs at the lumbar spine which may lead to early intervertebral disc damage. PMID:29065672

The Effects of Physiological Biomechanical Loading on Intradiscal Pressure and Annulus Stress in Lumbar Spine: A Finite Element Analysis.

PubMed

Zahari, Siti Nurfaezah; Latif, Mohd Juzaila Abd; Rahim, Nor Raihanah Abdull; Kadir, Mohammed Rafiq Abdul; Kamarul, Tunku

2017-01-01

The present study was conducted to examine the effects of body weight on intradiscal pressure (IDP) and annulus stress of intervertebral discs at lumbar spine. Three-dimensional finite element model of osseoligamentous lumbar spine was developed subjected to follower load of 500 N, 800 N, and 1200 N which represent the loads for individuals who are normal and overweight with the pure moments at 7.5 Nm in flexion and extension motions. It was observed that the maximum IDP was 1.26 MPa at L1-L2 vertebral segment. However, the highest increment of IDP was found at L4-L5 segment where the IDP was increased to 30% in flexion and it was more severe at extension motion reaching to 80%. Furthermore, the maximum annulus stress also occurred at the L1-L2 segment with 3.9 MPa in extension motion. However, the highest increment was also found at L4-L5 where the annulus stress increased to 17% in extension motion. Based on these results, the increase of physiological loading could be an important factor to the increment of intradiscal pressure and annulus fibrosis stress at all intervertebral discs at the lumbar spine which may lead to early intervertebral disc damage.

Chain Collapse of an Amyloidogenic Intrinsically Disordered Protein

PubMed Central

Jain, Neha; Bhattacharya, Mily; Mukhopadhyay, Samrat

2011-01-01

Natively unfolded or intrinsically disordered proteins (IDPs) are under intense scrutiny due to their involvement in both normal biological functions and abnormal protein misfolding disorders. Polypeptide chain collapse of amyloidogenic IDPs is believed to play a key role in protein misfolding, oligomerization, and aggregation leading to amyloid fibril formation, which is implicated in a number of human diseases. In this work, we used bovine κ-casein, which serves as an archetypal model protein for amyloidogenic IDPs. Using a variety of biophysical tools involving both prediction and spectroscopic techniques, we first established that monomeric κ-casein adopts a collapsed premolten-globule-like conformational ensemble under physiological conditions. Our time-resolved fluorescence and light-scattering data indicate a change in the mean hydrodynamic radius from ∼4.6 nm to ∼1.9 nm upon chain collapse. We then took the advantage of two cysteines separated by 77 amino-acid residues and covalently labeled them using thiol-reactive pyrene maleimide. This dual-labeled protein demonstrated a strong excimer formation upon renaturation from urea- and acid-denatured states under both equilibrium and kinetic conditions, providing compelling evidence of polypeptide chain collapse under physiological conditions. The implication of the IDP chain collapse in protein aggregation and amyloid formation is also discussed. PMID:21961598

Pristine Stratospheric Collections of Cosmic Dust

NASA Technical Reports Server (NTRS)

Messenger, S.; Keller, L. P.; Nakamura-Messenger, K.; Clemett, S. J.

2012-01-01

Since 1981, NASA has routinely collected interplanetary dust particles (IDPs) in the stratosphere by inertial impact onto silicone oil-coated flat plate collectors deployed on the wings of high-altitude aircraft [1]. The highly viscous oil traps and localizes the particles, which can fragment during collection. Particles are removed from the collectors with a micromanipulator and washed of the oil using organic solvents, typically hexane or xylene. While silicone oil is an efficient collection medium, its use is problematic. All IDPs are initially coated with this material (polydimethylsiloxane, n(CH3)2SiO) and traces of oil may remain after cleaning. The solvent rinse itself is also a concern as it likely removes indigenous organics from the particles. To avoid these issues, we used a polyurethane foam substrate for the oil-free stratospheric collection of IDPs.

Application of the maximum entropy principle to determine ensembles of intrinsically disordered proteins from residual dipolar couplings.

PubMed

Sanchez-Martinez, M; Crehuet, R

2014-12-21

We present a method based on the maximum entropy principle that can re-weight an ensemble of protein structures based on data from residual dipolar couplings (RDCs). The RDCs of intrinsically disordered proteins (IDPs) provide information on the secondary structure elements present in an ensemble; however even two sets of RDCs are not enough to fully determine the distribution of conformations, and the force field used to generate the structures has a pervasive influence on the refined ensemble. Two physics-based coarse-grained force fields, Profasi and Campari, are able to predict the secondary structure elements present in an IDP, but even after including the RDC data, the re-weighted ensembles differ between both force fields. Thus the spread of IDP ensembles highlights the need for better force fields. We distribute our algorithm in an open-source Python code.

CONFLICT IN EASTERN UKRAINE: STRATEGY FOR TUBERCULOSIS.

PubMed

Kuchuloria, T; Akhvlediani, T; Akhvlediani, N

2016-09-01

This root cause analysis concerns the conflict in the Eastern Ukraine and its impact on healthcare delivery in the context of treating internally displaced persons (IDPs). Inadequate treatment of tuberculosis (TB) was selected as a major topic for intervention planning in conflict areas in Ukraine. With respect to treating TB among IDPs, rapid diagnosis and adequate nutrition and shelter are important components of care and disease control. The DOT, supported by trained primary healthcare providers equipped with rapid MDR TB diagnostic capacities, need to provide appropriate shelter and nutrition to IDPs. In addition to active disease management, this paper discusses the important role of ongoing project monitoring and communicating evaluation findings with all the major stakeholders shaping the national TB strategy in Ukraine. A comprehensive strategy is essential for successful transitioning and re-structuring of TB healthcare delivery both during after conflict resolution.

Defining the safe current limit for opening ID photon shutter

DOE Office of Scientific and Technical Information (OSTI.GOV)

Seletskiy, S.

The NSLS-II storage ring is protected from possible damage from insertion devices (IDs) synchrotron radiation by a dedicated active interlock system (AIS). It monitors electron beam position and angle and triggers beam drop if beam orbit exceeds the boundaries of pre-calculated active interlock envelope (AIE). The beamlines (BL) and beamline frontends (FE) are designed under assumption that the electron beam is interlocked within the AIE. For historic reasons the AIS engages the ID active interlock (AI-ID) at any non-zero beam current whenever the ID photon shutter (IDPS) is getting opened. Such arrangement creates major inconveniences for BLs commissioning. Apparently theremore » is some IDPS safe current limit (SCL) under which the IDPS can be opened without interlocking the e-beam. The goal of this paper is to find such limit.« less

Radar-acoustic interaction for IFF applications

NASA Astrophysics Data System (ADS)

Saffold, James A.; Williamson, Frank R.; Ahuja, Krishan; Stein, Lawrence R.; Muller, Marjorie

1998-08-01

This paper describes the results of an internal development program (IDP) No. 97-1 conducted from August 1-October 1 1996 at the Georgia Tech Research Institute. The IDP program was implemented to establish theoretical relationships and verify the interaction between X-band radar waves and ultrasonic acoustics. Low cost, off-the-shelf components were used for the verification in order to illustrate the cost savings potential of developing and utilizing these systems. The measured data was used to calibrate the developed models of the phenomenology and to support extrapolation for radar systems which can exploit these interactions. One such exploitation is for soldier identification IFF and radar taggant concepts. The described IDP program provided the phenomenological data which is being used to extrapolate concept system performances based on technological limitations and battlefield conditions for low cost IFF and taggant configurations.

Fuel Efficient Stoves for Darfur Camps of Internally DisplacedPersons - Report of Field Trip to North and South Darfur, Nov. 16 -Dec.17, 2005

DOE Office of Scientific and Technical Information (OSTI.GOV)

Galitsky, Christina; Gadgil, Ashok; Jacobs, Mark

Approximately 2.2 million internally displaced persons (''IDPs'') in Darfur are living in dense camps scattered in arid areas with low fuelwood productivity. Unsustainable harvesting of fuelwood by the IDPs has created ever increasing zones of denudation, that now (in November 2005) have reached several kilometers from the camp boundaries. Leaving the safety of the camps to fetch fuelwood from farther and farther away imposes great risk and hardship on the IDP women. Three different metal fuel efficient stove (''FES'') designs were tested in Darfur IDP camps for their suitability to substantially reduce the fuelwood needs of IDPs. The mud-and-dung ''ITDG''more » stoves being promoted under the current FES program were also examined and tested. A modified design of the ITDG mud-and-dung stove, ''Avi'', was developed, built and tested. Systematic informal surveys of IDP households were undertaken in North and South Darfur to understand the household parameters related to family size, food, fuel, cooking habits, cooking pots, expenditure on fuel, and preferences related to alternative ways to spend time/money if fuel could be saved. Surveys found that a significant fraction of families are missing meals for lack of fuel (50% in South Darfur, and 90% in the North Darfur camps visited by the mission). About 60% of women in South Darfur, and about 90% of women in North Darfur camps purchase fuelwood. Selling some of the food rations to purchase fuel to cook meals was significant (40%) in South Darfur and has become common (80%) in North Darfur. The LBNL mission found that two of the metal stoves and the mud-and-dung Avi can significantly reduce fuelwood consumption using the same fuel, pot, cooking methods, and food ingredients used by Darfur IDPs. The most suitable design for Darfur conditions would be a modified ''Tara'' stove. With training of the cooks in tending the fire, this stove can save 50% fuel for the IDPs. The stove costs less than $10 (US) to produce in

Novel Experimental Simulations of the Atmospheric Injection of Meteoric Metals

NASA Astrophysics Data System (ADS)

Gómez Martín, J. C.; Bones, D. L.; Carrillo-Sánchez, J. D.; James, A. D.; Trigo-Rodríguez, J. M.; Fegley, B., Jr.; Plane, J. M. C.

2017-02-01

A newly developed laboratory, Meteoric Ablation Simulator (MASI), is used to test model predictions of the atmospheric ablation of interplanetary dust particles (IDPs) with experimental Na, Fe, and Ca vaporization profiles. MASI is the first laboratory setup capable of performing time-resolved atmospheric ablation simulations, by means of precision resistive heating and atomic laser-induced fluorescence detection. Experiments using meteoritic IDP analogues show that at least three mineral phases (Na-rich plagioclase, metal sulfide, and Mg-rich silicate) are required to explain the observed appearance temperatures of the vaporized elements. Low melting temperatures of Na-rich plagioclase and metal sulfide, compared to silicate grains, preclude equilibration of all the elemental constituents in a single melt. The phase-change process of distinct mineral components determines the way in which Na and Fe evaporate. Ca evaporation is dependent on particle size and on the initial composition of the molten silicate. Measured vaporized fractions of Na, Fe, and Ca as a function of particle size and speed confirm differential ablation (I.e., the most volatile elements such as Na ablate first, followed by the main constituents Fe, Mg, and Si, and finally the most refractory elements such as Ca). The Chemical Ablation Model (CABMOD) provides a reasonable approximation to this effect based on chemical fractionation of a molten silicate in thermodynamic equilibrium, even though the compositional and geometric description of IDPs is simplistic. Improvements in the model are required in order to better reproduce the specific shape of the elemental ablation profiles.

Novel Experimental Simulations of the Atmospheric Injection of Meteoric Metals

DOE Office of Scientific and Technical Information (OSTI.GOV)

Gómez Martín, J. C.; Bones, D. L.; Carrillo-Sánchez, J. D.

2017-02-20

A newly developed laboratory, Meteoric Ablation Simulator (MASI), is used to test model predictions of the atmospheric ablation of interplanetary dust particles (IDPs) with experimental Na, Fe, and Ca vaporization profiles. MASI is the first laboratory setup capable of performing time-resolved atmospheric ablation simulations, by means of precision resistive heating and atomic laser-induced fluorescence detection. Experiments using meteoritic IDP analogues show that at least three mineral phases (Na-rich plagioclase, metal sulfide, and Mg-rich silicate) are required to explain the observed appearance temperatures of the vaporized elements. Low melting temperatures of Na-rich plagioclase and metal sulfide, compared to silicate grains, precludemore » equilibration of all the elemental constituents in a single melt. The phase-change process of distinct mineral components determines the way in which Na and Fe evaporate. Ca evaporation is dependent on particle size and on the initial composition of the molten silicate. Measured vaporized fractions of Na, Fe, and Ca as a function of particle size and speed confirm differential ablation (i.e., the most volatile elements such as Na ablate first, followed by the main constituents Fe, Mg, and Si, and finally the most refractory elements such as Ca). The Chemical Ablation Model (CABMOD) provides a reasonable approximation to this effect based on chemical fractionation of a molten silicate in thermodynamic equilibrium, even though the compositional and geometric description of IDPs is simplistic. Improvements in the model are required in order to better reproduce the specific shape of the elemental ablation profiles.« less

Unusual olivine and pyroxene composition in interplanetary dust and unequilibrated ordinary chondrites

NASA Technical Reports Server (NTRS)

Klock, W.; Mckay, D. S.; Thomas, K. L.; Palme, H.

1989-01-01

The presence, in both a number of interplanetary dust particles (IDPs) and in meteorite matrices, of olivine and orthopyroxene grains, low in FeO but containing up to 5 wt pct MnO, is reported. The majority of olivines and pyroxenes in meteorites contain less than 0.5 wt pct MnO. The presence of these low-iron, manganese-enriched (LIME) olivines and pyroxenes in IDPs and meteorites may indicate a link between the origin and history of IDPs and the matrix material of primitive meteorites. The origin of the LIME silicates could be explained by condensation from a gas of solar composition. Forsterite is the first major silicate phase to condense from a solar nebula gas, and Mn, which is not stable as a metal under solar nebula conditions, would condense at about 1100 K as Mn2SiO4 in solid solution with forsterite.

Financing of internal displacement: excerpts from the Sri Lankan experience.

PubMed

Amirthalingam, Kopalapillai; Lakshman, Rajith W D

2010-04-01

This paper investigates how internally displaced persons (IDPs) in Batticaloa, Sri Lanka, financed their first year of displacement. We conducted extensive fieldwork in August 2007 in numerous welfare centres in Batticaloa that have received persons displaced from the Mutur divisional secretariat (DS) in the Trincomalee district. The sample was selected from the village of Sampur in Mutur DS. The displaced from Sampur are of Tamil origin and our findings reveal certain coping strategies that may be particular to this community, including the selling or mortgaging of gold jewellery to address the effects of displacement. We found that the IDPs in this region rely heavily on jewellery and other forms of savings to ensure their survival. There is also strong evidence to support the critically important coping role played by livelihoods. These coping strategies have enabled the IDP households in the present sample to live marginally above the poverty line.

Dancing Protein Clouds: The Strange Biology and Chaotic Physics of Intrinsically Disordered Proteins*

PubMed Central

2016-01-01

Biologically active but floppy proteins represent a new reality of modern protein science. These intrinsically disordered proteins (IDPs) and hybrid proteins containing ordered and intrinsically disordered protein regions (IDPRs) constitute a noticeable part of any given proteome. Functionally, they complement ordered proteins, and their conformational flexibility and structural plasticity allow them to perform impossible tricks and be engaged in biological activities that are inaccessible to well folded proteins with their unique structures. The major goals of this minireview are to show that, despite their simplified amino acid sequences, IDPs/IDPRs are complex entities often resembling chaotic systems, are structurally and functionally heterogeneous, and can be considered an important part of the structure-function continuum. Furthermore, IDPs/IDPRs are everywhere, and are ubiquitously engaged in various interactions characterized by a wide spectrum of binding scenarios and an even wider spectrum of structural and functional outputs. PMID:26851286

From Sequence and Forces to Structure, Function and Evolution of Intrinsically Disordered Proteins

PubMed Central

Forman-Kay, Julie D.; Mittag, Tanja

2015-01-01

Intrinsically disordered proteins (IDPs), which lack persistent structure, are a challenge to structural biology due to the inapplicability of standard methods for characterization of folded proteins as well as their deviation from the dominant structure/function paradigm. Their widespread presence and involvement in biological function, however, has spurred the growing acceptance of the importance of IDPs and the development of new tools for studying their structure, dynamics and function. The interplay of folded and disordered domains or regions for function and the existence of a continuum of protein states with respect to conformational energetics, motional timescales and compactness is shaping a unified understanding of structure-dynamics-disorder/function relationships. On the 20th anniversary of this journal, Structure, we provide a historical perspective on the investigation of IDPs and summarize the sequence features and physical forces that underlie their unique structural, functional and evolutionary properties. PMID:24010708

From sequence and forces to structure, function, and evolution of intrinsically disordered proteins.

PubMed

Forman-Kay, Julie D; Mittag, Tanja

2013-09-03

Intrinsically disordered proteins (IDPs), which lack persistent structure, are a challenge to structural biology due to the inapplicability of standard methods for characterization of folded proteins as well as their deviation from the dominant structure/function paradigm. Their widespread presence and involvement in biological function, however, has spurred the growing acceptance of the importance of IDPs and the development of new tools for studying their structure, dynamics, and function. The interplay of folded and disordered domains or regions for function and the existence of a continuum of protein states with respect to conformational energetics, motional timescales, and compactness are shaping a unified understanding of structure-dynamics-disorder/function relationships. In the 20(th) anniversary of Structure, we provide a historical perspective on the investigation of IDPs and summarize the sequence features and physical forces that underlie their unique structural, functional, and evolutionary properties. Copyright © 2013 Elsevier Ltd. All rights reserved.

Quantitative determination of the conformational properties of partially folded and intrinsically disordered proteins using NMR dipolar couplings.

PubMed

Jensen, Malene Ringkjøbing; Markwick, Phineus R L; Meier, Sebastian; Griesinger, Christian; Zweckstetter, Markus; Grzesiek, Stephan; Bernadó, Pau; Blackledge, Martin

2009-09-09

Intrinsically disordered proteins (IDPs) inhabit a conformational landscape that is too complex to be described by classical structural biology, posing an entirely new set of questions concerning the molecular understanding of functional biology. The characterization of the conformational properties of IDPs, and the elucidation of the role they play in molecular function, is therefore one of the major challenges remaining for modern structural biology. NMR is the technique of choice for studying this class of proteins, providing information about structure, flexibility, and interactions at atomic resolution even in completely disordered states. In particular, residual dipolar couplings (RDCs) have been shown to be uniquely sensitive and powerful tools for characterizing local and long-range structural behavior in disordered proteins. In this review we describe recent applications of RDCs to quantitatively describe the level of local structure and transient long-range order in IDPs involved in viral replication, neurodegenerative disease, and cancer.

Intrinsically Disordered Proteins as Important Players during Desiccation Stress of Soybean Radicles.

PubMed

Liu, Yun; Wu, Jiahui; Sun, Nan; Tu, Chengjian; Shi, Xiaoying; Cheng, Hua; Liu, Simu; Li, Shuiming; Wang, Yong; Zheng, Yizhi; Uversky, Vladimir N

2017-07-07

Intrinsically disordered proteins (IDPs) play a variety of important physiological roles in all living organisms. However, there is no comprehensive analysis of the abundance of IDPs associated with environmental stress in plants. Here, we show that a set of heat-stable proteins (i.e., proteins that do not denature after boiling at 100 °C for 10 min) was present in R 0mm and R 15mm radicles (i.e., before radicle emergence and 15 mm long radicles) of soybean (Glycine max) seeds. This set of 795 iTRAQ-quantified heat-stable proteins contained a high proportion of wholly or highly disordered proteins (15%), which was significantly higher than that estimated for the whole soybean proteome containing 55,787 proteins (9%). The heat-stable proteome of soybean radicles that contain many IDPs could protect lactate dehydrogenase (LDH) during freeze-thaw cycles. Comparison of the 795 heat-stable proteins in the R 0mm and R 15mm soybean radicles revealed that many of these proteins changed abundance during seedling growth with 170 and 89 proteins being more abundant in R 0mm and R 15mm , respectively. KEGG analysis identified 18 proteins from the cysteine and methionine metabolism pathways and nine proteins from the phenylpropanoid biosynthesis pathway. As an important type of IDP related to stress, 30 late embryogenesis abundant proteins were also found. Ten selected proteins with high levels of predicted intrinsic disorder were able to efficiently protect LDH from the freeze-thaw-induced inactivation, but the protective ability was not correlated with the disorder content of these proteins. These observations suggest that protection of the enzymes and other proteins in a stressed cell can be one of the biological functions of plant IDPs.

JPSS CGS Evolution

NASA Astrophysics Data System (ADS)

Miller, S. W.; Grant, K. D.; Jamilkowski, M. L.

2012-12-01

The National Oceanic and Atmospheric Administration (NOAA) and National Aeronautics and Space Administration (NASA) are jointly acquiring the next-generation civilian weather and environmental satellite system: the Joint Polar Satellite System (JPSS). JPSS will contribute the afternoon orbit component and ground processing system. As such, the Joint Polar Satellite System replaces the current Polar-orbiting Operational Environmental Satellites (POES) managed by NOAA. It also replaces the ground processing component of both Polar-orbiting Operational Environmental Satellites, as well as components of the Defense Meteorological Satellite Program (DMSP) replacement, previously known as the Defense Weather Satellite System (DWSS), managed by the Department of Defense (DoD). The JPSS satellites will carry a suite of sensors designed to collect meteorological, oceanographic, climatological and solar-geophysical observations of the earth, atmosphere and space. The ground processing system for JPSS is known as the JPSS Common Ground System (JPSS CGS), which consists of a Command, Control and Communications Segment (C3S) and an Interface Data Processing Segment (IDPS). Both segments are developed by Raytheon Intelligence and Information Systems (IIS). The C3S is currently flying the Suomi National Polar Partnership (Suomi NPP) satellite and transfers mission data from Suomi NPP and between the ground facilities. The IDPS processes Suomi NPP satellite data to provide Environmental Data Records (EDRs) to NOAA and DoD processing centers operated by the United States government. When the JPSS-1 satellite is launched in early 2017, the responsibilities of the C3S and the IDPS will be expanded to support both Suomi NPP and JPSS-1. The CGS also employs its ground stations at Svalbard, Norway and McMurdo Station, Antarctica, along with a global fiber communications network, to provide data acquisition and routing for multiple additional missions. These include POES, DMSP, NASA

Algorithm integration using ADL (Algorithm Development Library) for improving CrIMSS EDR science product quality

NASA Astrophysics Data System (ADS)

Das, B.; Wilson, M.; Divakarla, M. G.; Chen, W.; Barnet, C.; Wolf, W.

2013-05-01

Algorithm Development Library (ADL) is a framework that mimics the operational system IDPS (Interface Data Processing Segment) that is currently being used to process data from instruments aboard Suomi National Polar-orbiting Partnership (S-NPP) satellite. The satellite was launched successfully in October 2011. The Cross-track Infrared and Microwave Sounder Suite (CrIMSS) consists of the Advanced Technology Microwave Sounder (ATMS) and Cross-track Infrared Sounder (CrIS) instruments that are on-board of S-NPP. These instruments will also be on-board of JPSS (Joint Polar Satellite System) that will be launched in early 2017. The primary products of the CrIMSS Environmental Data Record (EDR) include global atmospheric vertical temperature, moisture, and pressure profiles (AVTP, AVMP and AVPP) and Ozone IP (Intermediate Product from CrIS radiances). Several algorithm updates have recently been proposed by CrIMSS scientists that include fixes to the handling of forward modeling errors, a more conservative identification of clear scenes, indexing corrections for daytime products, and relaxed constraints between surface temperature and air temperature for daytime land scenes. We have integrated these improvements into the ADL framework. This work compares the results from ADL emulation of future IDPS system incorporating all the suggested algorithm updates with the current official processing results by qualitative and quantitative evaluations. The results prove these algorithm updates improve science product quality.

Precision Oxygen Isotope Measurements of Two C-Rich Hydrated Interplanetary Dust Particles

NASA Technical Reports Server (NTRS)

Snead, C. J.; Keller, L. P.; McKeegan, K. D.; Messenger, S.

2016-01-01

Introduction: Chondritic-smooth IDPs (Interplanetary Dust Particles) are low porosity objects whose mineralogy is dominated by aqueous alteration products such as Mg-rich phyllosilicates (smectite and serpentine group) and Mg-Fe carbonate minerals. Their hydrated mineralogy combined with low atmospheric entry velocities have been used to infer an origin largely from asteroidal sources. Spectroscopic studies show that the types and abundance of organic matter in CS IDPs is similar to that in CP IDPs. Although CS IDPs show broad similarities to primitive carbonaceous chondrites, only a few particles have been directly linked to specific meteorite groups such as CM and CI chondrites based on the presence of diagnostic minerals. Many CS IDPs however, have carbon contents that greatly exceed that of known meteorite groups suggesting that they either may derive from comets or represent samples of more primitive parent bodies than do meteorites. It is now recognized that many large, dark primitive asteroids in the outer main belt, as well as some trans-Neptunian objects, show spectroscopic evidence for aqueous alteration products on their surfaces. Some CS IDPs exhibit large bulk D enrichments similar to those observed in the cometary CP IDPs. While hydrated minerals in comets have not been unambiguously identified to date, the presence of the smectite group mineral nontronite has been inferred from infrared spectra obtained from the ejecta from comet 9P/Tempel 1 during the Deep Impact mission. Recent observations of low temperature sulfide minerals in Stardust mission samples suggest that limited aqueous activity occurred on comet Wild-2. All of these observations, taken together, suggest that the high-carbon hydrated IDPs are abundant and important samples of primitive solar system objects not represented in meteorite collections. Oxygen isotopic compositions of chondrites reflect mixing between a 16O-rich reservoir and a 17O,18O-rich reservoir produced via mass

Frequent Spread of Plasmodium vivax Malaria Maintains High Genetic Diversity at the Myanmar-China Border, Without Distance and Landscape Barriers.

PubMed

Lo, Eugenia; Lam, Nancy; Hemming-Schroeder, Elizabeth; Nguyen, Jennifer; Zhou, Guofa; Lee, Ming-Chieh; Yang, Zhaoqing; Cui, Liwang; Yan, Guiyun

2017-12-05

In Myanmar, civil unrest and the establishment of internally displaced person (IDP) settlements along the Myanmar-China border have impacted malaria transmission. Microsatellite markers were used to examine source-sink dynamics for Plasmodium vivax between IDP settlements and surrounding villages in the border region. Genotypic structure and diversity were compared across the 3 years following the establishment of IDP settlements, to infer demographic history. We investigated whether human migration and landscape heterogeneity contributed to P. vivax transmission. P. vivax from IDP settlements and local communities consistently exhibited high genetic diversity within populations but low polyclonality within individuals. No apparent genetic structure was observed among populations and years. P. vivax genotypes in China were similar to those in Myanmar, and parasite introduction was unidirectional. Landscape factors, including distance, elevation, and land cover, do not appear to impede parasite gene flow. The admixture of P. vivax genotypes suggested that parasite gene flow via human movement contributes to the spread of malaria both locally in Myanmar and across the international border. Our genetic findings highlight the presence of large P. vivax gene reservoirs that can sustain transmission. Thus, it is important to reinforce and improve existing control efforts along border areas. © The Author 2017. Published by Oxford University Press for the Infectious Diseases Society of America. All rights reserved. For permissions, e-mail: journals.permissions@oup.com.

Accelerating the Conformational Sampling of Intrinsically Disordered Proteins.

PubMed

Do, Trang Nhu; Choy, Wing-Yiu; Karttunen, Mikko

2014-11-11

Intrinsically disordered proteins (IDPs) are a class of proteins lacking a well-defined secondary structure. Instead, they are able to attain multiple conformations, bind to multiple targets, and respond to changes in their surroundings. Functionally, IDPs have been associated with molecular recognition, cell regulation, and signal transduction. The dynamic conformational ensemble of IDPs is highly environmental and binding partner dependent, rendering the characterization of IDPs extremely challenging. Here, we compare the sampling efficiencies of conventional molecular dynamics (MD), well-tempered metadynamics (WT-META), and bias-exchange metadynamics (BE-META). The total simulation time was over 10 μs, and a 20-mer peptide derived from the Neh2 domain of the Nuclear factor erythroid 2-related factor 2 (Nrf2) protein was simulated. BE-META, with a neutral replica and seven biased replicas employing a set of seven relevant collective variables (CVs), provided the most reliable and efficient sampling. Finally, we propose a free-energy reconstruction method based on the probability distribution of the secondary structure contents. This postprocessing analysis confirms the presence of not only the β-hairpin conformation of the free Neh2 peptide but also its rare bound-state-like conformation, both of that have been experimentally observed. In addition, our simulations also predict other possible conformations to be verified with future experiments.

Computer modeling of Earthshine contamination on the VIIRS solar diffuser

NASA Astrophysics Data System (ADS)

Mills, Stephen P.; Agravante, Hiroshi; Hauss, Bruce; Klein, James E.; Weiss, Stephanie C.

2005-10-01

The Visible/Infrared Imager Radiometer Suite (VIIRS), built by Raytheon Santa Barbara Remote Sensing (SBRS) will be one of the primary earth-observing remote-sensing instruments on the National Polar-Orbiting Operational Environmental Satellite System (NPOESS). It will also be installed on the NPOESS Preparatory Project (NPP). These satellite systems fly in near-circular, sun-synchronous low-earth orbits at altitudes of approximately 830 km. VIIRS has 15 bands designed to measure reflectance with wavelengths between 412 nm and 2250 nm, and an additional 7 bands measuring primarily emissive radiance between 3700nm and 11450 nm. The calibration source for the reflective bands is a solar diffuser (SD) that is illuminated once per orbit as the satellite passes from the dark side to the light side of the earth near the poles. Sunlight enters VIIRS through an opening in the front of the instrument. An attenuation screen covers the opening, but other than this there are no other optical elements between the SD and the sun. The BRDF of the SD and the transmittance of the attenuation screen is measured pre-flight, and so with knowledge of the angles of incidence, the radiance of the sun can be computed and is used as a reference to produce calibrated reflectances and radiances. Unfortunately, the opening also allows a significant amount of reflected earthshine to illuminate part of the SD, and this component introduces radiometric error to the calibration process, referred to as earthshine contamination (ESC). The VIIRS radiometric error budget allocated a 0.3% error based on modeling of the ESC done by SBRS during the design phase. This model assumes that the earth has Lambertian BRDF with a maximum top-of-atmosphere albedo of 1. The Moderate Resolution Imaging Spectroradiometer (MODIS) has an SD with a design similar to VIIRS, and in 2003 the MODIS Science Team reported to Northrop Grumman Space Technology (NGST), the prime contractor for NPOESS, their suspicion that ESC

Characteristics and Trade-Offs of Doppler Lidar Global Wind Profiling

NASA Technical Reports Server (NTRS)

Kavaya, Michael J.; Emmitt, G David

2004-01-01

Accurate, global profiling of wind velocity is highly desired by NASA, NOAA, the DOD/DOC/NASA Integrated Program Office (IPO)/NPOESS, DOD, and others for many applications such as validation and improvement of climate models, and improved weather prediction. The most promising technology to deliver this measurement from space is Doppler Wind Lidar (DWL). The NASA/NOAA Global Tropospheric Wind Sounder (GTWS) program is currently in the process of generating the science requirements for a space-based sensor. In order to optimize the process of defining science requirements, it is important for the scientific and user community to understand the nature of the wind measurements that DWL can make. These measurements are very different from those made by passive imaging sensors or by active radar sensors. The purpose of this paper is to convey the sampling characteristics and data product trade-offs of an orbiting DWL.

Cometary dust: the diversity of primitive refractory grains

PubMed Central

Ishii, H. A.

2017-01-01

Comet dust is primitive and shows significant diversity. Our knowledge of the properties of primitive cometary particles has expanded significantly through microscale investigations of cosmic dust samples (anhydrous interplanetary dust particles (IDPs), chondritic porous (CP) IDPs and UltraCarbonaceous Antarctic micrometeorites, Stardust and Rosetta), as well as through remote sensing (Spitzer IR spectroscopy). Comet dust are aggregate particles of materials unequilibrated at submicrometre scales. We discuss the properties and processes experienced by primitive matter in comets. Primitive particles exhibit a diverse range of: structure and typology; distribution of constituents; concentration and form of carbonaceous and refractory organic matter; Mg- and Fe-contents of the silicate minerals; sulfides; existence/abundance of type II chondrule fragments; high-temperature calcium–aluminium inclusions and ameboid-olivine aggregates; and rarely occurring Mg-carbonates and magnetite, whose explanation requires aqueous alteration on parent bodies. The properties of refractory materials imply there were disc processes that resulted in different comets having particular selections of primitive materials. The diversity of primitive particles has implications for the diversity of materials in the protoplanetary disc present at the time and in the region where the comets formed. This article is part of the themed issue ‘Cometary science after Rosetta’. PMID:28554979

JPSS Cryosphere Algorithms: Integration and Testing in Algorithm Development Library (ADL)

NASA Astrophysics Data System (ADS)

Tsidulko, M.; Mahoney, R. L.; Meade, P.; Baldwin, D.; Tschudi, M. A.; Das, B.; Mikles, V. J.; Chen, W.; Tang, Y.; Sprietzer, K.; Zhao, Y.; Wolf, W.; Key, J.

2014-12-01

JPSS is a next generation satellite system that is planned to be launched in 2017. The satellites will carry a suite of sensors that are already on board the Suomi National Polar-orbiting Partnership (S-NPP) satellite. The NOAA/NESDIS/STAR Algorithm Integration Team (AIT) works within the Algorithm Development Library (ADL) framework which mimics the operational JPSS Interface Data Processing Segment (IDPS). The AIT contributes in development, integration and testing of scientific algorithms employed in the IDPS. This presentation discusses cryosphere related activities performed in ADL. The addition of a new ancillary data set - NOAA Global Multisensor Automated Snow/Ice data (GMASI) - with ADL code modifications is described. Preliminary GMASI impact on the gridded Snow/Ice product is estimated. Several modifications to the Ice Age algorithm that demonstrates mis-classification of ice type for certain areas/time periods are tested in the ADL. Sensitivity runs for day time, night time and terminator zone are performed and presented. Comparisons between the original and modified versions of the Ice Age algorithm are also presented.

GEMS Revealed: Spectrum Imaging of Aggregate Grains in Interplanetary Dust

NASA Technical Reports Server (NTRS)

Keller, L. P.; Messenger, S.; Christoffersen, R.

2005-01-01

Anhydrous interplanetary dust particles (IDPs) of cometary origin contain abundant materials that formed in the early solar nebula. These materials were transported outward and subsequently mixed with molecular cloud materials and presolar grains in the region where comets accreted [1]. GEMS (glass with embedded metal and sulfides) grains are a major component of these primitive anhydrous IDPs, along with crystalline Mg-rich silicates, Fe-Ni sulfides, carbonaceous material, and other trace phases. Some GEMS grains (5%) are demonstrably presolar based on their oxygen isotopic compositions [2]. However, most GEMS grains are isotopically solar and have bulk chemical compositions that are incompatible with inferred compositions of interstellar dust, suggesting a solar system origin [3]. An alternative hypothesis is that GEMS grains represent highly irradiated interstellar grains whose oxygen isotopic compositions were homogenized through processing in the interstellar medium (ISM) [4]. We have obtained the first quantitative X-ray maps (spectrum images) showing the distribution of major and minor elements in individual GEMS grains. Nanometer-scale chemical maps provide critical data required to evaluate the differing models regarding the origin of GEMS grains.

Hanford Internal Dosimetry Program Manual, PNL-MA-552

DOE Office of Scientific and Technical Information (OSTI.GOV)

Carbaugh, Eugene H.; Bihl, Donald E.; Maclellan, Jay A.

2003-10-10

This manual is a guide to the services provided by the Hanford Internal Dosimetry Program (IDP). It describes the roles of and relationships between the IDP and site contractors, and provides recommendations and guidance for consideration in implementing bioassay monitoring and internal dosimetry elements of radiation protection programs. Guidance includes identifying conditions under which workers should be placed on bioassay programs, types, descritptions, and capabilities of measurements, suggested routine bioassay programs, limitations on services, and practices for recording and reporting results.

Spin-State-Dependent Ion-Molecule Chemistry as the Origin of N-15 and D Isotopic Anomalies in Primitive Matter.

NASA Technical Reports Server (NTRS)

Wirstrom, E. S.; Charnley, S. B.; Cordiner, M. A.; Milam, S. N.

2012-01-01

Many meteoritic and interplanetary dust particle (IDP) samples contain bulk enhancements and hotspots rich in N-15. Similarly low C(14)N/C(15)N ratios have been observed in numerous comets, An almost constant enrichment factor in comets from disti'nct formation zones in the nebular disk (i.e. both Jupiter Family and Oort Cloud comets), strongly suggests that this fractionation is primordial and was set in the protsolar cloud core. Deuterium enrichment is observed in both meteorites and IDPs

Radiometric and spectral validation of Atmospheric Infrared Sounder observations with the aircraft-based Scanning High-Resolution Interferometer Sounder

NASA Astrophysics Data System (ADS)

Tobin, David C.; Revercomb, Henry E.; Knuteson, Robert O.; Best, Fred A.; Smith, William L.; Ciganovich, Nick N.; Dedecker, Ralph G.; Dutcher, Steven; Ellington, Scott D.; Garcia, Raymond K.; Howell, H. Benjamin; Laporte, Daniel D.; Mango, Stephen A.; Pagano, Thomas S.; Taylor, Joe K.; van Delst, Paul; Vinson, Kenneth H.; Werner, Mark W.

2006-05-01

The ability to accurately validate high-spectral resolution infrared radiance measurements from space using comparisons with a high-altitude aircraft spectrometer has been successfully demonstrated. The demonstration is based on a 21 November 2002 underflight of the AIRS on the NASA Aqua spacecraft by the Scanning-HIS on the NASA ER-2 high-altitude aircraft. A comparison technique which accounts for the different viewing geometries and spectral characteristics of the two sensors is introduced, and accurate comparisons are made for AIRS channels throughout the infrared spectrum. Resulting brightness temperature differences are found to be 0.2 K or less for most channels. Both the AIRS and the Scanning-HIS calibrations are expected to be very accurate (formal 3-sigma estimates are better than 1 K absolute brightness temperature for a wide range of scene temperatures), because high spectral resolution offers inherent advantages for absolute calibration and because they make use of high-emissivity cavity blackbodies as onboard radiometric references. AIRS also has the added advantage of a cold space view, and the Scanning-HIS calibration has recently benefited from the availability of a zenith view from high-altitude flights. Aircraft comparisons of this type provide a mechanism for periodically testing the absolute calibration of spacecraft instruments with instrumentation for which the calibration can be carefully maintained on the ground. This capability is especially valuable for assuring the long-term consistency and accuracy of climate observations, including those from the NASA EOS spacecraft (Terra, Aqua and Aura) and the new complement of NPOESS operational instruments. The validation role for accurately calibrated aircraft spectrometers also includes application to broadband instruments and linking the calibrations of similar instruments on different spacecraft. It is expected that aircraft flights of the Scanning-HIS and its close cousin the NPOESS Airborne

Dancing Protein Clouds: The Strange Biology and Chaotic Physics of Intrinsically Disordered Proteins.

PubMed

Uversky, Vladimir N

2016-03-25

Biologically active but floppy proteins represent a new reality of modern protein science. These intrinsically disordered proteins (IDPs) and hybrid proteins containing ordered and intrinsically disordered protein regions (IDPRs) constitute a noticeable part of any given proteome. Functionally, they complement ordered proteins, and their conformational flexibility and structural plasticity allow them to perform impossible tricks and be engaged in biological activities that are inaccessible to well folded proteins with their unique structures. The major goals of this minireview are to show that, despite their simplified amino acid sequences, IDPs/IDPRs are complex entities often resembling chaotic systems, are structurally and functionally heterogeneous, and can be considered an important part of the structure-function continuum. Furthermore, IDPs/IDPRs are everywhere, and are ubiquitously engaged in various interactions characterized by a wide spectrum of binding scenarios and an even wider spectrum of structural and functional outputs. © 2016 by The American Society for Biochemistry and Molecular Biology, Inc.

Identification of a Drug Targeting an Intrinsically Disordered Protein Involved in Pancreatic Adenocarcinoma

NASA Astrophysics Data System (ADS)

Neira, José L.; Bintz, Jennifer; Arruebo, María; Rizzuti, Bruno; Bonacci, Thomas; Vega, Sonia; Lanas, Angel; Velázquez-Campoy, Adrián; Iovanna, Juan L.; Abián, Olga

2017-01-01

Intrinsically disordered proteins (IDPs) are prevalent in eukaryotes, performing signaling and regulatory functions. Often associated with human diseases, they constitute drug-development targets. NUPR1 is a multifunctional IDP, over-expressed and involved in pancreatic ductal adenocarcinoma (PDAC) development. By screening 1120 FDA-approved compounds, fifteen candidates were selected, and their interactions with NUPR1 were characterized by experimental and simulation techniques. The protein remained disordered upon binding to all fifteen candidates. These compounds were tested in PDAC-derived cell-based assays, and all induced cell-growth arrest and senescence, reduced cell migration, and decreased chemoresistance, mimicking NUPR1-deficiency. The most effective compound completely arrested tumor development in vivo on xenografted PDAC-derived cells in mice. Besides reporting the discovery of a compound targeting an intact IDP and specifically active against PDAC, our study proves the possibility to target the ‘fuzzy’ interface of a protein that remains disordered upon binding to its natural biological partners or to selected drugs.

Identification of a Drug Targeting an Intrinsically Disordered Protein Involved in Pancreatic Adenocarcinoma

PubMed Central

Neira, José L.; Bintz, Jennifer; Arruebo, María; Rizzuti, Bruno; Bonacci, Thomas; Vega, Sonia; Lanas, Angel; Velázquez-Campoy, Adrián; Iovanna, Juan L.; Abián, Olga

2017-01-01

Intrinsically disordered proteins (IDPs) are prevalent in eukaryotes, performing signaling and regulatory functions. Often associated with human diseases, they constitute drug-development targets. NUPR1 is a multifunctional IDP, over-expressed and involved in pancreatic ductal adenocarcinoma (PDAC) development. By screening 1120 FDA-approved compounds, fifteen candidates were selected, and their interactions with NUPR1 were characterized by experimental and simulation techniques. The protein remained disordered upon binding to all fifteen candidates. These compounds were tested in PDAC-derived cell-based assays, and all induced cell-growth arrest and senescence, reduced cell migration, and decreased chemoresistance, mimicking NUPR1-deficiency. The most effective compound completely arrested tumor development in vivo on xenografted PDAC-derived cells in mice. Besides reporting the discovery of a compound targeting an intact IDP and specifically active against PDAC, our study proves the possibility to target the ‘fuzzy’ interface of a protein that remains disordered upon binding to its natural biological partners or to selected drugs. PMID:28054562

Clay minerals in primitive meteorites and interplanetary dust 1

NASA Technical Reports Server (NTRS)

Zolensky, M. E.; Keller, L. P.

1991-01-01

Many meteorites and interplanetary dust particles (IDPs) with primitive compositions contain significant amounts of phyllosilicate minerals, which are generally interpreted as evidence of protoplanetary aqueous alteration at an early period of the solar system. These meteorites are chondrites (near solar composition) of the carbonaceous and ordinary varieties. The former are subdivided (according to bulk composition and petrology) into CI, CM, CV, CO, CR, and ungrouped classes. IDPs are extraterrestrial particulates, collected in stratosphere, which have chemical compositions indicative of a primitive origin; they are typically distinct from the primitive meteorites. Characterization of phyllosilicates in these materials is a high priority because of the important physico-chemical information they hold. The most common phyllosilicates present in chondritic extraterrestrial materials are serpentine-group minerals, smectites, and micas. We discuss these phyllosilicates and describe the interpretation of their occurrence in meteorites and IDPs and what this indicates about history of their parent bodies, which are probably the hydrous asteroids.

Comparing the VIRTIS Spectrum of 67P/Churyumov-Gerasimenko to Wild 2 and in Primitive Interplanetary Dust Particles

NASA Astrophysics Data System (ADS)

Flynn, George

2016-04-01

The Visible and Infrared Thermal Imaging Spectrometer (VIRTIS) instrument, a point spectrometer with high spectral resolution covering the range from 2 to 5 microns, on the ESA Rosetta spacecraft obtained spectra of the surface of Comet 67P/Churyumov-Gerasimenko. The spectral region covered by VIRTIS has been well studied in meteorites, interplanetary dust particles (IDPs) collected by NASA from the Earth's stratosphere, and the samples of Comet 81P/Wild 2 that were delivered to Earth by the NASA Stardust spacecraft. Infrared spectra of the nucleus of Comet 67P/Churyumov-Gerasimenko acquired by VIRTIS show a broad absorption band observed at ~3.3 μm, a region of the spectrum where C-H, O-H, and N-H stretching features occur (Capaccioni et al., 2015). This broad feature is similar to the O-H feature exhibited by hydrous minerals, but shifted to a significantly longer wavelength. Capaccioni et al. (2015) compared the VIRTIS spectra to laboratory spectra of carbonaceous chondrite meteorites of the CI, CM, and CR types and concluded that none of the typical features of these meteorite spectra are compatible with the spectra of the surface of 67P/Churyumov-Gerasimenko. Comparison of VIRTIS spectra of 67P/ Churyumov-Gerasimenko to the laboratory spectra of well-characterized extraterrestrial materials including the Wild 2 dust and the IDPs, a significant fraction of which are believed to be cometary, could aid in the interpretation of the 67P/Churyumov-Gerasimenko spectra. None of the Wild 2 particles examined by infrared spectroscopy exhibit an O-H feature, but this may be due to the high temperature reached during their capture in the aerogel collection medium. The O-H feature is also absent in all anhydrous IDPs. The hydrous IDPs exhibit varying strengths of both aliphatic C-H absorption features and the O-H absorption feature, but, as with the meteorites, the O-H feature occurs at a significantly shorter wavelength than the broad feature detected in 67P

A Cloud-Based Infrastructure for Near-Real-Time Processing and Dissemination of NPP Data

NASA Astrophysics Data System (ADS)

Evans, J. D.; Valente, E. G.; Chettri, S. S.

2011-12-01

We are building a scalable cloud-based infrastructure for generating and disseminating near-real-time data products from a variety of geospatial and meteorological data sources, including the new National Polar-Orbiting Environmental Satellite System (NPOESS) Preparatory Project (NPP). Our approach relies on linking Direct Broadcast and other data streams to a suite of scientific algorithms coordinated by NASA's International Polar-Orbiter Processing Package (IPOPP). The resulting data products are directly accessible to a wide variety of end-user applications, via industry-standard protocols such as OGC Web Services, Unidata Local Data Manager, or OPeNDAP, using open source software components. The processing chain employs on-demand computing resources from Amazon.com's Elastic Compute Cloud and NASA's Nebula cloud services. Our current prototype targets short-term weather forecasting, in collaboration with NASA's Short-term Prediction Research and Transition (SPoRT) program and the National Weather Service. Direct Broadcast is especially crucial for NPP, whose current ground segment is unlikely to deliver data quickly enough for short-term weather forecasters and other near-real-time users. Direct Broadcast also allows full local control over data handling, from the receiving antenna to end-user applications: this provides opportunities to streamline processes for data ingest, processing, and dissemination, and thus to make interpreted data products (Environmental Data Records) available to practitioners within minutes of data capture at the sensor. Cloud computing lets us grow and shrink computing resources to meet large and rapid fluctuations in data availability (twice daily for polar orbiters) - and similarly large fluctuations in demand from our target (near-real-time) users. This offers a compelling business case for cloud computing: the processing or dissemination systems can grow arbitrarily large to sustain near-real time data access despite surges in

Comprehensive development and testing of the ASIST-GBV, a screening tool for responding to gender-based violence among women in humanitarian settings.

PubMed

Wirtz, A L; Glass, N; Pham, K; Perrin, N; Rubenstein, L S; Singh, S; Vu, A

2016-01-01

Conflict affected refugees and internally displaced persons (IDPs) are at increased vulnerability to gender-based violence (GBV). Health, psychosocial, and protection services have been implemented in humanitarian settings, but GBV remains under-reported and available services under-utilized. To improve access to existing GBV services and facilitate reporting, the ASIST-GBV screening tool was developed and tested for use in humanitarian settings. This process was completed in four phases: 1) systematic literature review, 2) qualitative research that included individual interviews and focus groups with GBV survivors and service providers, respectively, 3) pilot testing of the developed screening tool, and 4) 3-month implementation testing of the screening tool. Research was conducted among female refugees, aged ≥15 years in Ethiopia, and female IDPs, aged ≥18 years in Colombia. The systematic review and meta-analysis identified a range of GBV experiences and estimated a 21.4 % prevalence of sexual violence (95 % CI:14.9-28.7) among conflict-affected populations. No existing screening tools for GBV in humanitarian settings were identified. Qualitative research with GBV survivors in Ethiopia and Colombia found multiple forms of GBV experienced by refugees and IDPs that occurred during conflict, in transit, and in displaced settings. Identified forms of violence were combined into seven key items on the screening tool: threats of violence, physical violence, forced sex, sexual exploitation, forced pregnancy, forced abortion, and early or forced marriage. Cognitive testing further refined the tool. Pilot testing in both sites demonstrated preliminary feasibility where 64.8 % of participants in Ethiopia and 44.9 % of participants in Colombia were identified with recent (last 12 months) cases of GBV. Implementation testing of the screening tool, conducted as a routine service in camp/district hospitals, allowed for identification of GBV cases and referrals to

GEMS and New Pre-Accretionally Irradiated RELICT Grains in Interplanetary Dust - The Plot Thickens

NASA Astrophysics Data System (ADS)

Bradley, J.

1995-09-01

The hypothesis that GEMS (glass with embedded metal and sulfides) in interplanetary dust particles (IDPs) might be the long-sought interstellar silicate grains is undergoing close scrutiny [1-3]. GEMS are proposed to be interstellar because: (a) they are abundant in cometary IDPs; (b) they were irradiated prior to incorporation into IDPs; (c) both their size distribution and Oamorphous silicate" microstructures are consistent with those of interstellar silicates; (d) they contain nanometer-sized (superparamagnetic) alpha-iron inclusions, which provides a simple explanation for the observed interstellar grain alignment and polarization [4,5]. Challenges to the GEMS hypothesis include the following: (a) GEMS may have formed and been irradiated in the solar nebula rather than a presolar interstellar environment; (b) non-solar isotope abundances have yet to be measured in GEMS; (c) the irradiation regime required to produce the observed effects in GEMS might be incompatible with the interstellar medium; (b) relationships between GEMS and carbon (e.g. core/mantle) need clarification; (c) major element abundances in GEMS should be consistent with observed interstellar gas phase depletions [2,3]. GEMS may indeed have formed in the solar nebula, in which case they would be the oldest known solar nebula solids [2]. An interstellar origin for GEMS does not require detection of non-solar isotope abundances [6]. Irradiation experiments are in progress to simulate the properties of GEMS. The petrographic relationship between GEMS and carbon in IDPs is being investigated (by examining IDPs embedded and thin-sectioned in carbon-free media). Major element abundances in GEMS are being evaluated in terms on interstellar gas phase abundances. For example, sulfur is not highly depleted in the interstellar gas, implying that it must be significantly depleted in interstellar grains [3]. GEMS are significantly depleted in sulfur relative to solar abundances. Analytical electron

Hanford Internal Dosimetry Program Manual, PNL-MA-552

DOE Office of Scientific and Technical Information (OSTI.GOV)

Carbaugh, Eugene H.; Bihl, Donald E.; Maclellan, Jay A.

2009-09-24

This manual is a guide to the services provided by the Hanford Internal Dosimetry Program (IDP), which is operated by the Pacific Northwest National Laboratory.( ) for the U.S. Department of Energy Richland Operations Office, Office of River Protection and their Hanford Site contractors. The manual describes the roles of and relationships between the IDP and the radiation protection programs of the Hanford Site contractors. Recommendations and guidance are also provided for consideration in implementing bioassay monitoring and internal dosimetry elements of radiation protection programs.

Cometary and interstellar dust grains - Analysis by ion microprobe mass spectrometry and other techniques

NASA Technical Reports Server (NTRS)

Zinner, Ernst

1991-01-01

A survey of microanalytical measurements on interplanetary dust particles (IDPs) and interstellar dust grains from primitive meteorites is presented. Ion-microprobe mass spectrometry with its capability to determine isotopic compositions of many elements on a micron spatial scale has played a special role. Examples are measurements of H, N, and O isotopes and refractory trace elements in IDPs; C, N, Mg, and Si isotopes in interstellar SiC grains; and C and N isotopes and H, N, Al, and Si concentrations in interstellar graphite grains.

Multiple generations of grain aggregation in different environments preceded solar system body formation.

PubMed

Ishii, Hope A; Bradley, John P; Bechtel, Hans A; Brownlee, Donald E; Bustillo, Karen C; Ciston, James; Cuzzi, Jeffrey N; Floss, Christine; Joswiak, David J

2018-06-26

The solar system formed from interstellar dust and gas in a molecular cloud. Astronomical observations show that typical interstellar dust consists of amorphous ( a -) silicate and organic carbon. Bona fide physical samples for laboratory studies would yield unprecedented insight about solar system formation, but they were largely destroyed. The most likely repositories of surviving presolar dust are the least altered extraterrestrial materials, interplanetary dust particles (IDPs) with probable cometary origins. Cometary IDPs contain abundant submicron a- silicate grains called GEMS (glass with embedded metal and sulfides), believed to be carbon-free. Some have detectable isotopically anomalous a- silicate components from other stars, proving they are preserved dust inherited from the interstellar medium. However, it is debated whether the majority of GEMS predate the solar system or formed in the solar nebula by condensation of high-temperature (>1,300 K) gas. Here, we map IDP compositions with single nanometer-scale resolution and find that GEMS contain organic carbon. Mapping reveals two generations of grain aggregation, the key process in growth from dust grains to planetesimals, mediated by carbon. GEMS grains, some with a- silicate subgrains mantled by organic carbon, comprise the earliest generation of aggregates. These aggregates (and other grains) are encapsulated in lower-density organic carbon matrix, indicating a second generation of aggregation. Since this organic carbon thermally decomposes above ∼450 K, GEMS cannot have accreted in the hot solar nebula, and formed, instead, in the cold presolar molecular cloud and/or outer protoplanetary disk. We suggest that GEMS are consistent with surviving interstellar dust, condensed in situ, and cycled through multiple molecular clouds. Copyright © 2018 the Author(s). Published by PNAS.

Validation of S-NPP VIIRS Day-Night Band and M Bands Performance Using Ground Reference Targets of Libya 4 and Dome C

NASA Technical Reports Server (NTRS)

Chen, Xuexia; Wu, Aisheng; Xiong, Xiaoxiong; Lei, Ning; Wang, Zhipeng; Chiang, Kwofu

2015-01-01

This paper provides methodologies developed and implemented by the NASA VIIRS Calibration Support Team (VCST) to validate the S-NPP VIIRS Day-Night band (DNB) and M bands calibration performance. The Sensor Data Records produced by the Interface Data Processing Segment (IDPS) and NASA Land Product Evaluation and Algorithm Testing Element (PEATE) are acquired nearly nadir overpass for Libya 4 desert and Dome C snow surfaces. In the past 3.5 years, the modulated relative spectral responses (RSR) change with time and lead to 3.8% increase on the DNB sensed solar irradiance and 0.1% or less increases on the M4-M7 bands. After excluding data before April 5th, 2013, IDPS DNB radiance and reflectance data are consistent with Land PEATE data with 0.6% or less difference for Libya 4 site and 2% or less difference for Dome C site. These difference are caused by inconsistent LUTs and algorithms used in calibration. In Libya 4 site, the SCIAMACHY spectral and modulated RSR derived top of atmosphere (TOA) reflectance are compared with Land PEATE TOA reflectance and they indicate a decrease of 1.2% and 1.3%, respectively. The radiance of Land PEATE DNB are compared with the simulated radiance from aggregated M bands (M4, M5, and M7). These data trends match well with 2% or less difference for Libya 4 site and 4% or less difference for Dome C. This study demonstrate the consistent quality of DNB and M bands calibration for Land PEATE products during operational period and for IDPS products after April 5th, 2013.

Accretion of Interplanetary Dust Particles by the Earth

NASA Astrophysics Data System (ADS)

Kortenkamp, Stephen J.; Dermott, Stanley F.

1998-10-01

Analyses of hypervelocity micrometeoroid impact craters preserved in lunar material and on the panels of the Long Duration Exposure Facility (LDEF) indicate that each year Earth accretes about 3 × 107kg of interplanetary dust particles (IDPs) from the zodiacal cloud (E. Grünet al.1985,Astron. Astrophys.286, 915-924; S. G. Love and D. E. Brownlee, 1993,Science262, 550-553). The size distributions of these lunar and LDEF craters indicate that the mass distribution of IDPs encountering Earth peaks at about 200 μm diameter. This particle-size cutoff may be indicative of collisionally evolved asteroidal dust, where the collisional lifetime of dust particles larger than ∼100 μm is shorter than the time required for their orbits to decay under Poynting-Robertson light drag from the asteroid belt to Earth (B. Å. S. Gustafson, 1994,Annu. Rev. Earth Planet. Sci.22, 553-595). Additionally, analyses of IDPs collected from the stratosphere by high-flying aircraft reveal a diversity in chemical composition which is even narrower than that of the meteorites (G. J. Flynn, 1995,Nature376, 114). Together these findings suggest that IDPs present in the atmosphere and our collections may originate from very limited sources in the asteroid belt. The most abundant sources of dust to be unambiguously linked to the zodiacal cloud are the three asteroid families Eos, Themis, and Koronis-the progenitors of the ten-degree and low-latitude dust bands discovered by the Infrared Astronomical Satellite in 1984. We use direct numerical integration of the full equations of motion to model the orbital evolution of dust particles from these three families as well as from other nonfamily asteroids and from the population of known short period comets. Our simulations include gravitational perturbations from the planets, radiation pressure, and solar wind drag. We find that a large, and perhaps the dominant, fraction of the IDPs accreted by Earth comes from the asteroid families Eos, Themis, and

Fluid-induced organic synthesis in the solar nebula recorded in extraterrestrial dust from meteorites

PubMed Central

Vollmer, Christian; Kepaptsoglou, Demie; Leitner, Jan; Busemann, Henner; Spring, Nicole H.; Ramasse, Quentin M.; Hoppe, Peter; Nittler, Larry R.

2014-01-01

Isotopically anomalous carbonaceous grains in extraterrestrial samples represent the most pristine organics that were delivered to the early Earth. Here we report on gentle aberration-corrected scanning transmission electron microscopy investigations of eight 15N-rich or D-rich organic grains within two carbonaceous Renazzo-type (CR) chondrites and two interplanetary dust particles (IDPs) originating from comets. Organic matter in the IDP samples is less aromatic than that in the CR chondrites, and its functional group chemistry is mainly characterized by C–O bonding and aliphatic C. Organic grains in CR chondrites are associated with carbonates and elemental Ca, which originate either from aqueous fluids or possibly an indigenous organic source. One distinct grain from the CR chondrite NWA 852 exhibits a rim structure only visible in chemical maps. The outer part is nanoglobular in shape, highly aromatic, and enriched in anomalous nitrogen. Functional group chemistry of the inner part is similar to spectra from IDP organic grains and less aromatic with nitrogen below the detection limit. The boundary between these two areas is very sharp. The direct association of both IDP-like organic matter with dominant C–O bonding environments and nanoglobular organics with dominant aromatic and C–N functionality within one unique grain provides for the first time to our knowledge strong evidence for organic synthesis in the early solar system activated by an anomalous nitrogen-containing parent body fluid. PMID:25288736

Prolonged displacement may compromise resilience in Eritrean mothers.

PubMed

Almedom, Astier; Tesfamichael, Berhe; Mohammed, Zein; Mascie-Taylor, Nick; Muller, Jocelyn; Alemu, Zemui

2005-12-01

to assess the impact of prolonged displacement on the resilience of Eritrean mothers. an adapted SOC scale (short form) was administered. Complementary qualitative data were gathered from study participants' spontaneous reactions to and commentaries on the SOC scale. Displaced women's SOC scores were significantly less than those of the non-displaced: Mean = 54.84; SD = 6.48 in internally displaced person (IDP) camps, compared to non-displaced urban and rural/pastoralist: Mean = 48. 94, SD = 11.99; t = 3.831, p < .001. Post hoc tests revealed that the main difference is between IDP camp dwellers and urban (non-displaced). Rural but traditionally mobile (pastoralist or transhumant) communities scored more or less the same as the urban non-displaced--i.e., significantly higher than those in IDP camps (p < 0.05). Analysis of variance confirmed that gender is critical: displacement has significantly negative effects on women compared to men: RR = .262, p < .001. SOC scores of urban and pastoralist/transhumant groups were similar, while women in IDP camps were lower scoring--RR = .268, p < .001. The implications of these findings for health policy are critical. It is incumbent on the international health institutions including the World Health Organization and regional as well as local players to address the plight of internally displaced women, their families and communities in Eritrea and other places of dire conditions such as, for example Darfur in the Sudan.

Use of 'sense of coherence (SOC)' scale to measure resilience in Eritrea: interrogating both the data and the scale.

PubMed

Almedom, Astier M; Tesfamichael, Berhe; Saeed Mohammed, Zein; Mascie-Taylor, C G N; Alemu, Zemui

2007-01-01

An adapted 'sense of coherence' scale short form (SOC-13) was administered in nine languages of Eritrea with a total of 265 participants (162 women and 103 men) in order to assess 'resilience' in quantitative terms. Statistical analysis yielded significant differences in SOC scores between the displaced and non-displaced: mean=54.84 (SD=6.48) in internally displaced person (IDP) camps, compared with mean=48.94 (SD=11.99) in urban and rural settlements (t=3.831, p<0.001). Post-hoc tests revealed that the main difference is between IDP camp dwellers and urban (non-displaced) residents. Those in rural but traditionally mobile (pastoralist or transhumant) communities scored more or less the same as the urban non-displaced - i.e. significantly higher than those in IDP camps (p<0.05). Analysis of variance showed that displacement has a significantly negative effect on women compared with men (RR=0.262, p<0.001). Repeating the analysis for the three groups confirmed that urban and pastoralist/transhumant groups are similar, while women in IDP camps are lower scoring (RR=0.268, p<0.001), Hamboka women being worst affected due to their experience of serial displacement. These findings are interpreted and discussed in the light of qualitative information gleaned from the study participants' interrogation of the content of the SOC scale; and in the wider context of historical, socio-political and cultural characteristics of Eritrea. The study's implications for humanitarian and public health policy are considered.

Fluid-induced organic synthesis in the solar nebula recorded in extraterrestrial dust from meteorites.

PubMed

Vollmer, Christian; Kepaptsoglou, Demie; Leitner, Jan; Busemann, Henner; Spring, Nicole H; Ramasse, Quentin M; Hoppe, Peter; Nittler, Larry R

2014-10-28

Isotopically anomalous carbonaceous grains in extraterrestrial samples represent the most pristine organics that were delivered to the early Earth. Here we report on gentle aberration-corrected scanning transmission electron microscopy investigations of eight (15)N-rich or D-rich organic grains within two carbonaceous Renazzo-type (CR) chondrites and two interplanetary dust particles (IDPs) originating from comets. Organic matter in the IDP samples is less aromatic than that in the CR chondrites, and its functional group chemistry is mainly characterized by C-O bonding and aliphatic C. Organic grains in CR chondrites are associated with carbonates and elemental Ca, which originate either from aqueous fluids or possibly an indigenous organic source. One distinct grain from the CR chondrite NWA 852 exhibits a rim structure only visible in chemical maps. The outer part is nanoglobular in shape, highly aromatic, and enriched in anomalous nitrogen. Functional group chemistry of the inner part is similar to spectra from IDP organic grains and less aromatic with nitrogen below the detection limit. The boundary between these two areas is very sharp. The direct association of both IDP-like organic matter with dominant C-O bonding environments and nanoglobular organics with dominant aromatic and C-N functionality within one unique grain provides for the first time to our knowledge strong evidence for organic synthesis in the early solar system activated by an anomalous nitrogen-containing parent body fluid.

Fullerenes and interplanetary dust at the Permian-Triassic boundary.

PubMed

Poreda, Robert J; Becker, Luann

2003-01-01

We recently presented new evidence that an impact occurred approximately 250 million years ago at the Permian-Triassic boundary (PTB), triggering the most severe mass extinction in the history of life on Earth. We used a new extraterrestrial tracer, fullerene, a third carbon carrier of noble gases besides diamond and graphite. By exploiting the unique properties of this molecule to trap noble gases inside of its caged structure (helium, neon, argon), the origin of the fullerenes can be determined. Here, we present new evidence for fullerenes with extraterrestrial noble gases in the PTB at Graphite Peak, Antarctica, similar to PTB fullerenes from Meishan, China and Sasayama, Japan. In addition, we isolated a (3)He-rich magnetic carrier phase in three fractions from the Graphite Peak section. The noble gases in this magnetic fraction were similar to zero-age deep-sea interplanetary dust particles (IDPs) and some magnetic grains isolated from the Cretaceous-Tertiary boundary. The helium and neon isotopic compositions for both the bulk Graphite Peak sediments and an isolated magnetic fraction from the bulk material are consistent with solar-type gases measured in zero-age deep-sea sediments and point to a common source, namely, the flux of IDPs to the Earth's surface. In this instance, the IDP noble gas signature for the bulk sediment can be uniquely decoupled from fullerene, demonstrating that two separate tracers are present (direct flux of IDPs for (3)He vs. giant impact for fullerene).

DOE Office of Scientific and Technical Information (OSTI.GOV)

Matrajt, G.; Flynn, G.; Brownlee, D.

We analyzed two ultracarbonaceous interplanetary dust particles (IDPs) and two cometary Wild 2 particles with infrared spectroscopy. We characterized the carrier of the 3.4 {mu}m band in these samples and compared its profile and the CH{sub 2}/CH{sub 3} ratios to the 3.4 {mu}m band in the diffuse interstellar medium (DISM), in the insoluble organic matter from three primitive meteorites, in asteroid 24 Themis, and in the coma of comet 103P/Hartley 2. We found that the 3.4 {mu}m band in both Wild 2 and IDPs is similar, but different from all of the other astrophysical environments that we compared it to.more » The 3.4 {mu}m band in the IDPs and Wild 2 particles is dominated by CH{sub 2} groups, the peaks are narrower and stronger than in the meteorites, asteroid Themis, and the DISM. Also, the presence of the carbonyl group C=O at {approx}1700 cm{sup -1} (5.8 {mu}m) in most of the spectra of our samples indicates that these aliphatic chains have O bonded to them, which is quite different from astronomical spectra of the DISM. Based on all of these observations, we conclude that the origin of the carrier of the 3.4 {mu}m band in the IDPs and Wild 2 samples is not interstellar; instead, we suggest that the origin lies in the outermost parts of the solar nebula.« less

NMR contributions to structural dynamics studies of intrinsically disordered proteins☆

PubMed Central

Konrat, Robert

2014-01-01

Intrinsically disordered proteins (IDPs) are characterized by substantial conformational plasticity. Given their inherent structural flexibility X-ray crystallography is not applicable to study these proteins. In contrast, NMR spectroscopy offers unique opportunities for structural and dynamic studies of IDPs. The past two decades have witnessed significant development of NMR spectroscopy that couples advances in spin physics and chemistry with a broad range of applications. This article will summarize key advances in basic physical-chemistry and NMR methodology, outline their limitations and envision future R&D directions. PMID:24656082

Automated analysis of retinal images for detection of referable diabetic retinopathy.

PubMed

Abràmoff, Michael D; Folk, James C; Han, Dennis P; Walker, Jonathan D; Williams, David F; Russell, Stephen R; Massin, Pascale; Cochener, Beatrice; Gain, Philippe; Tang, Li; Lamard, Mathieu; Moga, Daniela C; Quellec, Gwénolé; Niemeijer, Meindert

2013-03-01

The diagnostic accuracy of computer detection programs has been reported to be comparable to that of specialists and expert readers, but no computer detection programs have been validated in an independent cohort using an internationally recognized diabetic retinopathy (DR) standard. To determine the sensitivity and specificity of the Iowa Detection Program (IDP) to detect referable diabetic retinopathy (RDR). In primary care DR clinics in France, from January 1, 2005, through December 31, 2010, patients were photographed consecutively, and retinal color images were graded for retinopathy severity according to the International Clinical Diabetic Retinopathy scale and macular edema by 3 masked independent retinal specialists and regraded with adjudication until consensus. The IDP analyzed the same images at a predetermined and fixed set point. We defined RDR as more than mild nonproliferative retinopathy and/or macular edema. A total of 874 people with diabetes at risk for DR. Sensitivity and specificity of the IDP to detect RDR, area under the receiver operating characteristic curve, sensitivity and specificity of the retinal specialists' readings, and mean interobserver difference (κ). The RDR prevalence was 21.7% (95% CI, 19.0%-24.5%). The IDP sensitivity was 96.8% (95% CI, 94.4%-99.3%) and specificity was 59.4% (95% CI, 55.7%-63.0%), corresponding to 6 of 874 false-negative results (none met treatment criteria). The area under the receiver operating characteristic curve was 0.937 (95% CI, 0.916-0.959). Before adjudication and consensus, the sensitivity/specificity of the retinal specialists were 0.80/0.98, 0.71/1.00, and 0.91/0.95, and the mean intergrader κ was 0.822. The IDP has high sensitivity and specificity to detect RDR. Computer analysis of retinal photographs for DR and automated detection of RDR can be implemented safely into the DR screening pipeline, potentially improving access to screening and health care productivity and reducing visual loss

Contribution of industrial density and socioeconomic status to the spatial distribution of thyroid cancer risk in Hangzhou, China.

PubMed

Fei, Xufeng; Lou, Zhaohan; Christakos, George; Liu, Qingmin; Ren, Yanjun; Wu, Jiaping

2018-02-01

The thyroid cancer (TC) incidence in China has increased dramatically during the last three decades. Typical in this respect is the case of Hangzhou city (China), where 7147 new TC cases were diagnosed during the period 2008-2012. Hence, the assessment of the TC incidence risk increase due to environmental exposure is an important public health matter. Correlation analysis, Analysis of Variance (ANOVA) and Poisson regression were first used to evaluate the statistical association between TC and key risk factors (industrial density and socioeconomic status). Then, the Bayesian maximum entropy (BME) theory and the integrative disease predictability (IDP) criterion were combined to quantitatively assess both the overall and the spatially distributed strength of the "exposure-disease" association. Overall, higher socioeconomic status was positively correlated with higher TC risk (Pearson correlation coefficient=0.687, P<0.01). Compared to people of low socioeconomic status, people of median and high socioeconomic status showed higher TC risk: the Relative Risk (RR) and associated 95% confidence interval (CI) were found to be, respectively, RR=2.29 with 95% CI=1.99 to 2.63, and RR=3.67 with 95% CI=3.22 to 4.19. The "industrial density-TC incidence" correlation, however, was non-significant. Spatially, the "socioeconomic status-TC" association measured by the corresponding IDP coefficient was significant throughout the study area: the mean IDP value was -0.12 and the spatial IDP values were consistently negative at the township level. It was found that stronger associations were distributed among residents mainly on a stripe of land from northeast to southwest (consisting mainly of sub-district areas). The "industrial density-TC" association measured by its IDP coefficient was spatially non-consistent. Socioeconomic status is an important indicator of TC risk factor in Hangzhou (China) whose effect varies across space. Hence, socioeconomic status shows the highest TC

Thermodynamic characterization of the multivalent interactions underlying rapid and selective translocation through the nuclear pore complex

PubMed Central

Hayama, Ryo; Sparks, Samuel; Hecht, Lee M.; Dutta, Kaushik; Karp, Jerome M.; Cabana, Christina M.; Rout, Michael P.; Cowburn, David

2018-01-01

Intrinsically disordered proteins (IDPs) play important roles in many biological systems. Given the vast conformational space that IDPs can explore, the thermodynamics of the interactions with their partners is closely linked to their biological functions. Intrinsically disordered regions of Phe–Gly nucleoporins (FG Nups) that contain multiple phenylalanine–glycine repeats are of particular interest, as their interactions with transport factors (TFs) underlie the paradoxically rapid yet also highly selective transport of macromolecules mediated by the nuclear pore complex. Here, we used NMR and isothermal titration calorimetry to thermodynamically characterize these multivalent interactions. These analyses revealed that a combination of low per-FG motif affinity and the enthalpy–entropy balance prevents high-avidity interaction between FG Nups and TFs, whereas the large number of FG motifs promotes frequent FG–TF contacts, resulting in enhanced selectivity. Our thermodynamic model underlines the importance of functional disorder of FG Nups. It helps explain the rapid and selective translocation of TFs through the nuclear pore complex and further expands our understanding of the mechanisms of “fuzzy” interactions involving IDPs. PMID:29374059

Measurement of polycyclic aromatic hydrocarbon (PAHs) in interplanetary dust particles

NASA Technical Reports Server (NTRS)

Clemett, S. J.; Maechling, C. R.; Zare, R. N.; Swan, P. D.; Walker, R. M.

1993-01-01

We report here the first definitive measurements of specific organic molecules (polycyclic aromatic hydrocarbons (PAH's)) in interplanetary dust particles (IDP's). An improved version of the microbeam-two-step laser mass spectrometer was used for the analysis. Two IDP's gave similar mass spectra showing an abundance of PAH's. Control samples, including particles of probable terrestrial origin from the same stratospheric collector, gave either null results or quite different spectra. We conclude that the PAH's are probably indigenous to the IDP's and are not terrestrial contaminants. The instrument used to study the particles is a two-step laser mass spectrometer. Constituent neutral molecules of the sample are first desorbed with a pulsed infrared laser beam focussed to 40 micrometers. In the second step, PAH's in the desorbed plume are preferentially ionized by a pulsed UV laser beam. Resulting ions produced by resonant absorption are extracted into a reflectron time-of-flight mass spectrometer. This instrument has high spatial resolution, high ion transmission, unlimited mass range, and multichannel detection of all ion masses from a single laser shot.

Binding of Disordered Peptides to Kelch: Insights from Enhanced Sampling Simulations.

PubMed

Do, Trang Nhu; Choy, Wing-Yiu; Karttunen, Mikko

2016-01-12

Keap1 protein plays an essential role in regulating cellular oxidative stress response and is a crucial binding hub for multiple proteins, several of which are intrinsically disordered proteins (IDP). Among Kelch's IDP binding partners, NRF2 and PTMA are the two most interesting cases. They share a highly similar binding motif; however, NRF2 binds to Kelch with a binding affinity of approximately 100-fold higher than that of PTMA. In this study, we perform an exhaustive sampling composed of 6 μs well-tempered metadynamics and 2 μs unbiased molecular dynamics (MD) simulations aiming at characterizing the binding mechanisms and structural properties of these two peptides. Our results agree with previous experimental observations that PTMA is remarkably more disordered than NRF2 in both the free and bound states. This explains PTMA's lower binding affinity. Our extensive sampling also provides valuable insights into the vast conformational ensembles of both NRF2 and PTMA, supports the hypothesis of coupled folding-binding, and confirms the essential role of linear motifs in IDP binding.

Impact of Ionic Liquids on the Structure and Dynamics of Collagen.

PubMed

Tarannum, Aafiya; Adams, Alina; Blümich, Bernhard; Fathima, Nishter Nishad

2018-01-25

The changes in the structure and dynamics of collagen treated with two different classes of ionic liquids, bis-choline sulfate (CS) and 1-butyl-3-methyl imidazolium dimethyl phosphate (IDP), have been studied at the molecular and fibrillar levels. At the molecular level, circular dichroic studies revealed an increase in molar ellipticity values for CS when compared with native collagen, indicating cross-linking, albeit pronounced conformational changes for IDP were witnessed indicating denaturation. The impedance was analyzed to correlate the conformational changes with the hydration dynamics of protein. Changes in the dielectric properties of collagen observed upon treatment with CS and IDP reported molecular reorientation in the surrounding water milieu, suggesting compactness or destabilization of the collagen. This was further confirmed by proton transverse NMR relaxation time measurements, which demonstrated that the water mobility changes in the presence of the ILs. At the fibrillar level, differential scanning calorimetry thermograms for rat tail tendon collagen fibers treated with CS show a 5 °C increase in denaturation temperature, suggesting imparted stability. On the contrary, a significant temperature decrease was noticed for IDP, indicating the destabilization of collagen fibers. The obtained results clearly indicate that the changes in the secondary structure of protein are due to the changes in the hydration dynamics of collagen upon interaction with ILs. Thus, this study on the interaction of collagen with ionic liquids unfolds the propensity of ILs to stabilize or destabilize collagen depending on the changes invoked at the molecular level in terms of structure and dynamics of protein, which also got manifested at the fibrillar level.

Equation Chapter 1 Section 1Sequence-To-Conformation Relationships of Disordered Regions Tethered to Folded Domains of Proteins.

PubMed

Mittal, Anuradha; Holehouse, Alex S; Cohan, Megan C; Pappu, Rohit V

2018-05-12

Intrinsically disordered proteins and regions (IDPs / IDRs) are characterized by well-defined sequence-to-conformation relationships (SCRs). These relationships refer to the sequence-specific preferences for average sizes, shapes, residue-specific secondary structure propensities, and amplitudes of multiscale conformational fluctuations. SCRs are discerned from the sequence-specific conformational ensembles of IDPs. A vast majority of IDPs are actually tethered to folded domains (FDs). This raises the question of whether or not SCRs inferred for IDPs are applicable to IDRs tethered to folded domains. Here, we use atomistic simulations based on a well-established forcefield paradigm and an enhanced sampling method to obtain comparative assessments of SCRs for thirteen archetypal IDRs modeled as autonomous units, as C-terminal tails connected to folded domains, and as linkers between pairs of folded domains. Our studies uncover a set of general observations regarding context-independent versus context-dependent SCRs of IDRs. SCRs are minimally perturbed upon tethering to folded domains if the IDRs are deficient in charged residues and for polyampholytic IDRs where the oppositely charged residues within the sequence of the IDR are separated into distinct blocks. In contrast, the interplay between IDRs and tethered folded domains has a significant modulatory effect on SCRs if the IDRs have intermediate fractions of charged residues or if they have sequence-intrinsic conformational preferences for canonical random coils. Our findings suggest that IDRs with context-independent SCRs might be independent evolutionary modules whereas IDRs with context-dependent intrinsic SCRs might co-evolve with the FDs to which they are tethered. Copyright © 2018. Published by Elsevier Ltd.

Cloning of a cDNA encoding bovine mitochondrial NADP(+)-specific isocitrate dehydrogenase and structural comparison with its isoenzymes from different species.

PubMed Central

Huh, T L; Ryu, J H; Huh, J W; Sung, H C; Oh, I U; Song, B J; Veech, R L

1993-01-01

Mitochondrial NADP(+)-specific isocitrate dehydrogenase (IDP) was co-purified with the pyruvate dehydrogenase complex from bovine kidney mitochondria. The determination of its N-terminal 16-amino-acid sequence revealed that it is highly similar to the IDP from yeast. A cDNA clone (1.8 kb long) encoding this protein was isolated from a bovine kidney lambda gt11 cDNA library using a synthetic oligodeoxynucleotide. The deduced protein sequence of this cDNA clone rendered a precursor protein of 452 amino-acid residues (50,830 Da) and a mature protein of 413 amino-acid residues (46,519 Da). It is 100% identical to the internal tryptic peptide sequences of the autologous form from pig heart and 62% similar to that from yeast. However, it shares little similarity with the mitochondrial NAD(+)-specific isoenzyme from yeast. Structural analyses of the deduced proteins of IDP isoenzymes from different species indicated that similarity exists in certain regions, which may represent the common domains for the active sites or coenzyme-binding sites. In Northern-blot analysis, one species of mRNA (about 2.2 kb for both bovine and human) was hybridized with a 32P-labelled cDNA probe. Southern-blot analysis of genomic DNAs verified simple patterns of hybridization with this cDNA. These results strongly indicate that the mitochondrial IDP may be derived from a single gene family which does not appear to be closely related to that of the NAD(+)-specific isoenzyme. Images Figure 1 Figure 3 Figure 4 Figure 5 PMID:8318002

Spontaneous Fluctuations can Guide Drug Design Strategies for Structurally Disordered Proteins.

PubMed

Maity, Barun Kumar; Vishvakarma, Vicky; Surendran, Dayana; Rawat, Anoop; Das, Anirban; Pramanik, Shreya; Arfin, Najmul; Maiti, Sudipta

2018-06-21

Structure-based 'rational' drug-design strategies fail for diseases associated with intrinsically disordered proteins (IDPs). However, structural disorder allows large amplitude spontaneous intramolecular dynamics in a protein. We demonstrate a method that exploits this dynamics to provide quantitative information about the degree of interaction of an IDP with other molecules. A candidate ligand molecule may not bind strongly, but even momentary interactions can be expected to perturb the fluctuations. We measure the amplitude and frequency of the equilibrium fluctuations of fluorescently labeled small oligomers of hIAPP (an IDP associated with Type II diabetes) in a physiological solution, using nanosecond fluorescence cross-correlation spectroscopy. We show that the inter-terminal distance fluctuates at a characteristic timescale of 134 ± 10 ns, and 6.4 ± 0.2 % of the population is in the 'closed' (quenched) state at equilibrium. These fluctuations are affected in a dose-dependent manner by a series of small molecules known to reduce the toxicity of various amyloid peptides. The degree of interaction shows the following order: resveratrol < epicatechin ~ quercetin < congo red < epigallocatechin-3-gallate. Such ordering can provide a direction for exploring the chemical space for finding stronger-binding ligands. We test the biological relevance of these measurements by measuring the effect of these molecules on the affinity of hIAPP for lipid vesicles and cell membranes. We find that the ability of a molecule to modulate intramolecular fluctuations correlates well with its ability to lower membrane affinity. We conclude that structural disorder may provide new avenues for rational drug design for IDPs.

Clinical outcome following DIAM implantation for symptomatic lumbar internal disk disruption: a 3-year retrospective analysis

PubMed Central

Lu, Kang; Liliang, Po-Chou; Wang, Hao-Kuang; Chen, Jui-Sheng; Chen, Te-Yuan; Huang, Ruyi; Chen, Han-Jung

2016-01-01

Background/objective Internal disk disruption (IDD), an early event of lumbar disk degeneration, is the most common cause of low back pain. Since increased intradiskal pressure (IDP) is associated with symptoms and progression of disk degeneration, unloading a painful disk with an interspinous process device (IPD) is a rational treatment option. The goal of this study was to evaluate the effectiveness of dynamic stabilization with an IPD in the treatment of symptomatic IDD of the lumbar spine. Patients and methods Patients with symptomatic IDD were treated with implantation of an IPD, the device for intervertebral assisted motion (DIAM). Diagnosis of IDD was based on typical MRI finding of posterior annular high-intensity zone and positive provocative test on discography. IDP was analyzed intraoperatively. Axial back and leg pain was evaluated with visual analog scale, functional status with Oswestry Disability Index, and final clinical outcomes with Odom criteria. Data from 34 patients followed up for at least 3 years were collected. Results DIAM implantation significantly reduced IDP (n=11, P<0.0001). All 34 patients reported symptom relief. Thirty-one patients (91%) remained symptom free until the last followups. Three patients (9%) experienced recurrence of pain, of which the causes were unrelated to the IDD or surgery. Disk status at the DIAM-implanted segments remained stable. Segmental flexion/extension mobility was preserved in 27 of 30 patients with preoperative mobility. No proximal or distal adjacent segment degeneration was observed. The final clinical outcomes were excellent/good in 31 and fair/poor in three patients. Conclusion For patients with symptomatic IDD, dynamic stabilization with DIAM provides pain relief and functional improvement. The implantation maintains disk status and prevents progression of disk degeneration, without compromising segmental flexion/extension mobility or causing adjacent segment degeneration. PMID:27826214

Interfacial Properties of NTAIL, an Intrinsically Disordered Protein.

PubMed

Bénarouche, Anaïs; Habchi, Johnny; Cagna, Alain; Maniti, Ofelia; Girard-Egrot, Agnès; Cavalier, Jean-François; Longhi, Sonia; Carrière, Frédéric

2017-12-19

Intrinsically disordered proteins (IDPs) lack stable secondary and tertiary structure under physiological conditions in the absence of their biological partners and thus exist as dynamic ensembles of interconverting conformers, often highly soluble in water. However, in some cases, IDPs such as the ones involved in neurodegenerative diseases can form protein aggregates and their aggregation process may be triggered by the interaction with membranes. Although the interfacial behavior of globular proteins has been extensively studied, experimental data on IDPs at the air/water (A/W) and water/lipid interfaces are scarce. We studied here the intrinsically disordered C-terminal domain of the Hendra virus nucleoprotein (N TAIL ) and compared its interfacial properties to those of lysozyme that is taken as a model globular protein of similar molecular mass. Adsorption of N TAIL at the A/W interface was studied in the absence and presence of phospholipids using Langmuir films, polarization modulated-infrared reflection-absorption spectroscopy, and an automated drop tensiometer for interfacial tension and elastic modulus determination with oscillating bubbles. N TAIL showed a significant surface activity, with a higher adsorption capacity at the A/W interface and penetration into egg phosphatidylcholine monolayer compared to lysozyme. Whereas lysozyme remains folded upon compression of the protein layer at the A/W interface and shows a quasi-pure elastic behavior, N TAIL shows a much higher molecular area and forms a highly viscoelastic film with a high dilational modulus. To our knowledge, a new disorder-to-order transition is thus observed for the N TAIL protein that folds into an antiparallel β-sheet at the A/W interface and presents strong intermolecular interactions. Copyright © 2017 Biophysical Society. Published by Elsevier Inc. All rights reserved.

Transitioning GONG data processing to NOAA SWPC operations

NASA Astrophysics Data System (ADS)

Reinard, A.; Marble, A.; Hill, F.; Berger, T. E.

2015-12-01

The NOAA Space Weather Prediction Center (SWPC) is the nation's official source of space weather watches, warnings, and alerts, providing 24x7 forecasting and support to critical infrastructure operators around the world. Observations of the conditions on the Sun are crucial for determining when and if a warning is needed. The Global Oscillation Network Group (GONG) operated by the National Solar Observatory (NSO) consists of six ground stations, allowing continuous observations of the Sun. Of particular interest for space weather purposes are the H-alpha images and magnetograms. The H-alpha data are used to identify filaments and their eruptions, to assess active region evolution and plage extent, and to help localize flare locations. The magnetograms are used to identify neutral lines, to examine potential shearing areas and to characterize the magnetic structure of active regions. GONG magnetograms also provide the initial condition for models of solar wind expansion through the heliosphere such as the WSA-Enlil model. Although beyond the scope of current space weather applications, GONG helioseismology products can be used to assess active region emergence on the far side of the Sun and to indicate the flaring potential of a front-side active region. These products are being examined as future tools in flare prediction. NSO has operated GONG as a science facility since 1995 and has provided processed space weather data products to NOAA via public internet connections for the past several years. In 2014 the White House Office of Management and Budget (OMB) requested that NOAA transition the GONG network to an operational space weather asset in order to ensure the continued flow of critical magnetogram data for solar wind models. NSO will continue to operate and manage the instruments and sites, but the H-alpha images and 10 minute averaged magnetogram data will be sent directly to SWPC for processing and use in space weather modeling. SWPC will make these data

Transitioning GONG data processing to NOAA SWPC operations

NASA Astrophysics Data System (ADS)

Reinard, Alysha; Marble, Andrew R.; Berger, Thomas

2016-05-01

The NOAA Space Weather Prediction Center (SWPC) is the nation's official source of space weather watches, warnings, and alerts, providing 24x7 forecasting and support to critical infrastructure operators around the world. Observations of the conditions on the Sun are crucial for determining when and if a warning is needed. The Global Oscillation Network Group (GONG) operated by the National Solar Observatory (NSO) consists of six ground stations, allowing continuous observations of the Sun. Of particular interest for space weather purposes are the H-alpha images and magnetograms. The H-alpha data are used to identify filaments and their eruptions, to assess active region evolution and plage extent, and to help localize flare locations. The magnetograms are used to identify neutral lines, to examine potential shearing areas and to characterize the magnetic structure of active regions. GONG magnetograms also provide the initial condition for models of solar wind expansion through the heliosphere such as the WSA-Enlil model. Although beyond the scope of current space weather applications, GONG helioseismology products can be used to assess active region emergence on the far side of the Sun and to indicate the flaring potential of a front-side active region. These products are being examined as future tools in flare prediction.NSO has operated GONG as a science facility since 1995 and has provided processed space weather data products to NOAA via for the past several years. In 2014 the White House Office of Management and Budget (OMB) requested that NOAA transition the GONG network to an operational space weather asset in order to ensure the continued flow of critical data for solar wind models. NSO will continue to operate and manage the instruments and sites, but the H-alpha images and 10 minute averaged magnetogram data will be sent directly to SWPC for processing and use in space weather modeling. SWPC will make these data available to NSO and the public via the

Mineralogy of Interplanetary Dust Particles from the Comet Giacobini-Zinner Dust Stream Collections

NASA Technical Reports Server (NTRS)

Nakamura-Messenger, K.; Messenger, S.; Westphal, A. J.; Palma, R. L.

2015-01-01

The Draconoid meteor shower, originating from comet 21P/Giacobini-Zinner, is a low-velocity Earth-crossing dust stream that had a peak anticipated flux on Oct. 8, 2012. In response to this prediction, NASA performed dedicated stratospheric dust collections to target interplanetary dust particles (IDPs) from this comet stream on Oct 15-17, 2012 [3]. Twelve dust particles from this targeted collection were allocated to our coordinated analysis team for studies of noble gas (Univ. Minnesota, Minnesota State Univ.), SXRF and Fe-XANES (SSL Berkeley) and mineralogy/isotopes (JSC). Here we report a mineralogical study of 3 IDPs from the Draconoid collection..

Meteorological Satellite Education Resources: Web-based Learning Modules, Initiatives, and the Environmental Satellite Resource Center (ESRC)

NASA Astrophysics Data System (ADS)

Schreiber-Abshire, W.; Dills, P.

2008-12-01

The COMET® Program (www.comet.ucar.edu) receives funding from NOAA NESDIS and the NPOESS Integrated Program Office (IPO), with additional contributions from the GOES-R Program Office and EUMETSAT, to directly support education and training efforts in the area of satellite meteorology. This partnership enables COMET to create educational materials of global interest on geostationary and polar- orbiting remote sensing platforms and their instruments, data, products, and operational applications. Over the last several years, COMET's satellite education programs have focused on the capabilities and applications of the upcoming next generation operational polar-orbiting NPP/NPOESS system and its relevance to operational forecasters and other user communities. COMET's activities have recently expanded to include education on the future Geostationary Operational Environmental Satellites (GOES-R). By partnering with experts from the Naval Research Laboratory, NOAA-NESDIS and various user communities, COMET stimulates greater utilization of both current and future satellite observations and products. In addition, COMET has broadened the scope of its online training to include materials on the EUMETSAT Polar-orbiting System (EPS) and Meteosat geostationary satellites. EPS represents an important contribution to the Initial Joint Polar System (IJPS) between NOAA and EUMETSAT, while Meteosat imaging capabilities provide an early look for the next generation GOES-R satellites. Also in collaboration with EUMETSAT, COMET is developing future modules on the joint NASA-CNES Jason altimetry mission and on satellite capabilities for monitoring the global climate. COMET also provides Spanish translations of relevant GOES materials in order to support the GEOSS (Global Earth Observation System of Systems) Americas effort, which is associated with the move of GOES-10 to provide routine satellite coverage over South America. This poster presentation provides an overview of COMET

Validation of OMPS Ozone Profile Data with Expanded Dataset from Brewer and Automated Dobson Network.

NASA Astrophysics Data System (ADS)

Petropavlovskikh, I.; Weatherhead, E.; Cede, A.; Oltmans, S. J.; Kireev, S.; Maillard, E.; Bhartia, P. K.; Flynn, L. E.

2005-12-01

The first NPOESS satellite is scheduled to be launched in 2010 and will carry the Ozone Mapping and Profiler Suite (OMPS) instruments for ozone monitoring. Prior this, the OMPS instruments and algorithms will be tested by flight on the NPOESS/NPP satellite, scheduled for launch in 2008. Pre-launch planning for validation, post launch data validation and verification of the nadir and limb profile algorithm are key components for insuring that the NPOESS will produce a high quality, reliable ozone profile data set. The heritage of satellite instrument validation (TOMS, SBUV, GOME, SCIAMACHY, SAGE, HALOE, ATMOS, etc) has always relied upon surface-based observations. While the global coverage of satellite observations is appealing for validating another satellite, there is no substitute for the hard reference point of a ground-based system such as the Dobson or Brewer network, whose instruments are routinely calibrated and intercompared to standard references. The standard solar occultation instruments, SAGE II and HALOE are well beyond their planned lifetimes and might be inoperative during the OMPS period. The Umkehr network has been one of the key data sets for stratospheric ozone trend calculations and has earned its place as a benchmark network for stratospheric ozone profile observations. The normalization of measurements at different solar zenith angle (SZAs) to the measurement at the smallest SZA cancels out many calibration parameters, including the extra-terrestrial solar flux and instrumental constant, thus providing a "self-calibrating" technique in the same manner relied upon by the occultation sensors on satellites. Moreover, the ground-based Umkehr measurement is the only technique that provides data with the same altitude resolution and in the same units (DU) as do the UV-nadir instruments (SBUV-2, GOME-2, OMPS-nadir), i.e., as ozone amount in pressure layers, whereas, occultation instruments measure ozone density with height. A new Umkehr algorithm

On Using SysML, DoDAF 2.0 and UPDM to Model the Architecture for the NOAA's Joint Polar Satellite System (JPSS) Ground System (GS)

NASA Technical Reports Server (NTRS)

Hayden, Jeffrey L.; Jeffries, Alan

2012-01-01

The JPSS Ground System is a lIexible system of systems responsible for telemetry, tracking & command (TT &C), data acquisition, routing and data processing services for a varied lIeet of satellites to support weather prediction, modeling and climate modeling. To assist in this engineering effort, architecture modeling tools are being employed to translate the former NPOESS baseline to the new JPSS baseline, The paper will focus on the methodology for the system engineering process and the use of these architecture modeling tools within that process, The Department of Defense Architecture Framework version 2,0 (DoDAF 2.0) viewpoints and views that are being used to describe the JPSS GS architecture are discussed. The Unified Profile for DoOAF and MODAF (UPDM) and Systems Modeling Language (SysML), as ' provided by extensions to the MagicDraw UML modeling tool, are used to develop the diagrams and tables that make up the architecture model. The model development process and structure are discussed, examples are shown, and details of handling the complexities of a large System of Systems (SoS), such as the JPSS GS, with an equally complex modeling tool, are described

Proteus: a random forest classifier to predict disorder-to-order transitioning binding regions in intrinsically disordered proteins

NASA Astrophysics Data System (ADS)

Basu, Sankar; Söderquist, Fredrik; Wallner, Björn

2017-05-01

The focus of the computational structural biology community has taken a dramatic shift over the past one-and-a-half decades from the classical protein structure prediction problem to the possible understanding of intrinsically disordered proteins (IDP) or proteins containing regions of disorder (IDPR). The current interest lies in the unraveling of a disorder-to-order transitioning code embedded in the amino acid sequences of IDPs/IDPRs. Disordered proteins are characterized by an enormous amount of structural plasticity which makes them promiscuous in binding to different partners, multi-functional in cellular activity and atypical in folding energy landscapes resembling partially folded molten globules. Also, their involvement in several deadly human diseases (e.g. cancer, cardiovascular and neurodegenerative diseases) makes them attractive drug targets, and important for a biochemical understanding of the disease(s). The study of the structural ensemble of IDPs is rather difficult, in particular for transient interactions. When bound to a structured partner, an IDPR adapts an ordered conformation in the complex. The residues that undergo this disorder-to-order transition are called protean residues, generally found in short contiguous stretches and the first step in understanding the modus operandi of an IDP/IDPR would be to predict these residues. There are a few available methods which predict these protean segments from their amino acid sequences; however, their performance reported in the literature leaves clear room for improvement. With this background, the current study presents `Proteus', a random forest classifier that predicts the likelihood of a residue undergoing a disorder-to-order transition upon binding to a potential partner protein. The prediction is based on features that can be calculated using the amino acid sequence alone. Proteus compares favorably with existing methods predicting twice as many true positives as the second best method (55

Factors associated with the health status of internally displaced persons in northern Uganda

PubMed Central

Roberts, B; Ocaka, K Felix; Browne, J; Oyok, T; Sondorp, E

2009-01-01

Background: Globally, there are over 24 million internally displaced persons (IDPs) who have fled their homes due to violence and insecurity but who remain within their own country. There have been up to 2 million IDPs in northern Uganda alone. The objective of this study was to investigate factors associated with mental and physical health status of IDPs in northern Uganda. Methods: A cross-sectional survey was conducted in November 2006 in IDP camps in the Gulu and Amuru districts of northern Uganda. The study outcome of physical and mental health was measured using the SF-8 instrument, which produces physical (PCS) and mental (MCS) component summary measures. Independent demographic, socio-economic, and trauma exposure (using the Harvard Trauma Questionnaire) variables were also measured. Multivariate regression linear regression analysis was conducted to investigate associations of the independent variables on the PCS and MCS outcomes. Results: 1206 interviews were completed. The respective mean PCS and MCS scores were 42.2 (95% CI 41.32 to 43.10) and 39.3 (95% CI 38.42 to 40.13), well below the instrument norm of 50, indicating poor health. Variables with negative associations with physical or mental health included gender, age, marital status, income, distance of camp from home areas, food security, soap availability, and sense of safety in the camp. A number of individual trauma variables and the frequency of trauma exposure also had negative associations with physical and mental health. Conclusions: This study provides evidence on the impact on health of deprivation of basic goods and services, traumatic events, and fear and uncertainty amongst displaced and crisis affected populations. PMID:19028730

The impact of internal displacement on child mortality in post-earthquake Haiti: a difference-in-differences analysis.

PubMed

Chen, Bradley; Halliday, Timothy J; Fan, Victoria Y

2016-07-19

The Haiti earthquake in 2010 resulted in 1.5 million internally displaced people (IDP), yet little is known about the impact of displacement on health. In this study, we estimate the impact of displacement on infant and child mortality and key health-behavior mechanisms. We employ a difference-in-differences (DID) design with coarsened exact matching (CEM) to ensure comparability among groups with different displacement status using the 2012 Haiti Demographic and Health Survey (DHS). The participants are 21,417 births reported by a nationally representative sample of 14,287 women aged 15-49. The main independent variables are household displacement status which includes households living in camps, IDP households (not in camps), and households not displaced. The main outcomes are infant and child mortality; health status (height-for-age, anemia); uptake of public health interventions (bed net use, spraying against mosquitoes, and vaccinations); and other conditions (hunger; cholera). Births from the camp households have higher infant mortality (OR = 2.34, 95 % CI 1.15 to 4.75) and child mortality (OR = 2.34, 95 % CI 1.10 to 5.00) than those in non-camp IDP households following the earthquake. These odds are higher despite better access to food, water, bed net use, mosquito spraying, and vaccines among camp households. IDP populations are heterogeneous and households that are displaced outside of camps may be self-selected or self-insured. Meanwhile, even households not displaced by a disaster may face challenges in access to basic necessities and health services. Efforts are needed to identify vulnerable populations to provide targeted assistance in post-disaster relief.

Nutrition among men and household food security in an internally displaced persons camp in Kenya.

PubMed

Singh, Kamal P; Bhoopathy, Sankara Varun; Worth, Heather; Seale, Holly; Richmond, Robyn L

2016-03-01

To determine the nutritional status of men and the food security status of their households in an internally displaced persons (IDP) camp in Kenya. A descriptive, cross-sectional study using a questionnaire and biometric measurements was completed in June 2013. IDP camp, Rongai, Kenya. A total of 267 men aged ≥18 years residing within the camp were recruited via respondent-driven sampling. Statistical associations between categorical variables were analysed using Pearson's χ 2 tests, while independent t tests were used for continuous variables. Among the men surveyed, we found a mean BMI of 20·3 (sd 2·5) kg/m2, with 23·9% of participants in the underweight category (BMI<18·5 kg/m2). The mean Individual Dietary Diversity Score was 6 out of a maximum score of 9. The mean Household Food Insecurity Access Scale score was 11·6 (sd 6·8), with 180 participants (71·7%) residing in households categorised as severely food insecure. Low monthly household income (<2000 Kenyan Shillings, or $US 25) was associated with a higher food insecurity score (P<0·001), greater likelihood of residing in a severely food-insecure household (P<0·001), low dietary diversity score (P<0·05) and being underweight (P<0·01). While the nutritional status of men in the IDP camp is comparable to non-displaced men in Kenya, household food insecurity is relatively high. Efforts to improve food security for the future are essential to minimise the impact of severe food insecurity on mental health, disease profiles and family well-being reported in other IDP settings.

Bisphosphonate Inhibitors Reveal a Large Elasticity of Plastidic Isoprenoid Synthesis Pathway in Isoprene-Emitting Hybrid Aspen1

PubMed Central

2015-01-01

Recently, a feedback inhibition of the chloroplastic 1-deoxy-d-xylulose 5-phosphate (DXP)/2-C-methyl-d-erythritol 4-phosphate (MEP) pathway of isoprenoid synthesis by end products dimethylallyl diphosphate (DMADP) and isopentenyl diphosphate (IDP) was postulated, but the extent to which DMADP and IDP can build up is not known. We used bisphosphonate inhibitors, alendronate and zoledronate, that inhibit the consumption of DMADP and IDP by prenyltransferases to gain insight into the extent of end product accumulation and possible feedback inhibition in isoprene-emitting hybrid aspen (Populus tremula × Populus tremuloides). A kinetic method based on dark release of isoprene emission at the expense of substrate pools accumulated in light was used to estimate the in vivo pool sizes of DMADP and upstream metabolites. Feeding with fosmidomycin, an inhibitor of DXP reductoisomerase, alone or in combination with bisphosphonates was used to inhibit carbon input into DXP/MEP pathway or both input and output. We observed a major increase in pathway intermediates, 3- to 4-fold, upstream of DMADP in bisphosphonate-inhibited leaves, but the DMADP pool was enhanced much less, 1.3- to 1.5-fold. In combined fosmidomycin/bisphosphonate treatment, pathway intermediates accumulated, reflecting cytosolic flux of intermediates that can be important under strong metabolic pull in physiological conditions. The data suggested that metabolites accumulated upstream of DMADP consist of phosphorylated intermediates and IDP. Slow conversion of the huge pools of intermediates to DMADP was limited by reductive energy supply. These data indicate that the DXP/MEP pathway is extremely elastic, and the presence of a significant pool of phosphorylated intermediates provides an important valve for fine tuning the pathway flux. PMID:25926480

Factors associated with post-traumatic stress disorder and depression amongst internally displaced persons in northern Uganda

PubMed Central

Roberts, Bayard; Ocaka, Kaducu Felix; Browne, John; Oyok, Thomas; Sondorp, Egbert

2008-01-01

Background The 20 year war in northern Uganda between the Lord's Resistance Army and the Ugandan government has resulted in the displacement of up to 2 million people within Uganda. The purpose of the study was to measure rates of post-traumatic stress disorder (PTSD) and depression amongst these internally displaced persons (IDPs), and investigate associated demographic and trauma exposure risk factors. Methods A cross-sectional multi-staged, random cluster survey with 1210 adult IDPs was conducted in November 2006 in Gulu and Amuru districts of northern Uganda. Levels of exposure to traumatic events and PTSD were measured using the Harvard Trauma Questionnaire (original version), and levels of depression were measured using the Hopkins Symptom Checklist-25. Multivariate logistic regression was used to analyse the association of demographic and trauma exposure variables on the outcomes of PTSD and depression. Results Over half (54%) of the respondents met symptom criteria for PTSD, and over two thirds (67%) of respondents met symptom criteria for depression. Over half (58%) of respondents had experienced 8 or more of the 16 trauma events covered in the questionnaire. Factors strongly linked with PTSD and depression included gender, marital status, distance of displacement, experiencing ill health without medical care, experiencing rape or sexual abuse, experiencing lack of food or water, and experiencing higher rates of trauma exposure. Conclusion This study provides evidence of exposure to traumatic events and deprivation of essential goods and services suffered by IDPs, and the resultant effect this has upon their mental health. Protection and social and psychological assistance are urgently required to help IDPs in northern Uganda re-build their lives. PMID:18489768

Multimode Jahn-Teller effect in bulk systems: A case of the N V 0 center in diamond

DOE PAGES

Zhang, Jianhua; Wang, Cai -Zhuang; Zhu, Zizhong; ...

2018-04-15

Here, the multimode Jahn-Teller (JT) effect in a bulk system of a neutral nitrogen-vacancy (NV 0) center in diamond is investigated via first-principles density-functional-theory calculations and the intrinsic distortion path (IDP) method. The adiabatic potential energy surface of the electronic ground state of the NV 0 center is calculated based on the local spin-density approximation. Our calculations confirm the presence of the dynamic Jahn-Teller effect in the ground 2E state of the NV 0 center. Within the harmonic approximation, the IDP method provides the reactive path of JT distortion from unstable high-symmetry geometry to stable low-symmetry energy minimum geometry, andmore » it describes the active normal modes participating in the distortion. We find that there is more than one vibrational mode contributing to the distortion, and their contributions change along the IDP. Several vibrational modes with large contributions to JT distortion, especially those modes close to 44 meV, are clearly observed as the phonon sideband in photoluminescence spectra in a series of experiments, indicating that the dynamic Jahn-Teller effect plays an important role in the optical transition of the NV 0 center.« less

Multimode Jahn-Teller effect in bulk systems: A case of the N V0 center in diamond

NASA Astrophysics Data System (ADS)

Zhang, Jianhua; Wang, Cai-Zhuang; Zhu, Zizhong; Liu, Qing Huo; Ho, Kai-Ming

2018-04-01

The multimode Jahn-Teller (JT) effect in a bulk system of a neutral nitrogen-vacancy (N V0 ) center in diamond is investigated via first-principles density-functional-theory calculations and the intrinsic distortion path (IDP) method. The adiabatic potential energy surface of the electronic ground state of the N V0 center is calculated based on the local spin-density approximation. Our calculations confirm the presence of the dynamic Jahn-Teller effect in the ground 2E state of the N V0 center. Within the harmonic approximation, the IDP method provides the reactive path of JT distortion from unstable high-symmetry geometry to stable low-symmetry energy minimum geometry, and it describes the active normal modes participating in the distortion. We find that there is more than one vibrational mode contributing to the distortion, and their contributions change along the IDP. Several vibrational modes with large contributions to JT distortion, especially those modes close to 44 meV, are clearly observed as the phonon sideband in photoluminescence spectra in a series of experiments, indicating that the dynamic Jahn-Teller effect plays an important role in the optical transition of the N V0 center.

Optimization algorithms for large-scale multireservoir hydropower systems

DOE Office of Scientific and Technical Information (OSTI.GOV)

Hiew, K.L.

Five optimization algorithms were vigorously evaluated based on applications on a hypothetical five-reservoir hydropower system. These algorithms are incremental dynamic programming (IDP), successive linear programing (SLP), feasible direction method (FDM), optimal control theory (OCT) and objective-space dynamic programming (OSDP). The performance of these algorithms were comparatively evaluated using unbiased, objective criteria which include accuracy of results, rate of convergence, smoothness of resulting storage and release trajectories, computer time and memory requirements, robustness and other pertinent secondary considerations. Results have shown that all the algorithms, with the exception of OSDP converge to optimum objective values within 1.0% difference from one another.more » The highest objective value is obtained by IDP, followed closely by OCT. Computer time required by these algorithms, however, differ by more than two orders of magnitude, ranging from 10 seconds in the case of OCT to a maximum of about 2000 seconds for IDP. With a well-designed penalty scheme to deal with state-space constraints, OCT proves to be the most-efficient algorithm based on its overall performance. SLP, FDM, and OCT were applied to the case study of Mahaweli project, a ten-powerplant system in Sri Lanka.« less

Multimode Jahn-Teller effect in bulk systems: A case of the N V 0 center in diamond

DOE Office of Scientific and Technical Information (OSTI.GOV)

Zhang, Jianhua; Wang, Cai -Zhuang; Zhu, Zizhong

Here, the multimode Jahn-Teller (JT) effect in a bulk system of a neutral nitrogen-vacancy (NV 0) center in diamond is investigated via first-principles density-functional-theory calculations and the intrinsic distortion path (IDP) method. The adiabatic potential energy surface of the electronic ground state of the NV 0 center is calculated based on the local spin-density approximation. Our calculations confirm the presence of the dynamic Jahn-Teller effect in the ground 2E state of the NV 0 center. Within the harmonic approximation, the IDP method provides the reactive path of JT distortion from unstable high-symmetry geometry to stable low-symmetry energy minimum geometry, andmore » it describes the active normal modes participating in the distortion. We find that there is more than one vibrational mode contributing to the distortion, and their contributions change along the IDP. Several vibrational modes with large contributions to JT distortion, especially those modes close to 44 meV, are clearly observed as the phonon sideband in photoluminescence spectra in a series of experiments, indicating that the dynamic Jahn-Teller effect plays an important role in the optical transition of the NV 0 center.« less

Structural disorder in plant proteins: where plasticity meets sessility.

PubMed

Covarrubias, Alejandra A; Cuevas-Velazquez, Cesar L; Romero-Pérez, Paulette S; Rendón-Luna, David F; Chater, Caspar C C

2017-09-01

Plants are sessile organisms. This intriguing nature provokes the question of how they survive despite the continual perturbations caused by their constantly changing environment. The large amount of knowledge accumulated to date demonstrates the fascinating dynamic and plastic mechanisms, which underpin the diverse strategies selected in plants in response to the fluctuating environment. This phenotypic plasticity requires an efficient integration of external cues to their growth and developmental programs that can only be achieved through the dynamic and interactive coordination of various signaling networks. Given the versatility of intrinsic structural disorder within proteins, this feature appears as one of the leading characters of such complex functional circuits, critical for plant adaptation and survival in their wild habitats. In this review, we present information of those intrinsically disordered proteins (IDPs) from plants for which their high level of predicted structural disorder has been correlated with a particular function, or where there is experimental evidence linking this structural feature with its protein function. Using examples of plant IDPs involved in the control of cell cycle, metabolism, hormonal signaling and regulation of gene expression, development and responses to stress, we demonstrate the critical importance of IDPs throughout the life of the plant.

Hydrodynamic Radii of Intrinsically Disordered Proteins Determined from Experimental Polyproline II Propensities

PubMed Central

Tomasso, Maria E.; Tarver, Micheal J.; Devarajan, Deepa; Whitten, Steven T.

2016-01-01

The properties of disordered proteins are thought to depend on intrinsic conformational propensities for polyproline II (PP II) structure. While intrinsic PP II propensities have been measured for the common biological amino acids in short peptides, the ability of these experimentally determined propensities to quantitatively reproduce structural behavior in intrinsically disordered proteins (IDPs) has not been established. Presented here are results from molecular simulations of disordered proteins showing that the hydrodynamic radius (R h) can be predicted from experimental PP II propensities with good agreement, even when charge-based considerations are omitted. The simulations demonstrate that R h and chain propensity for PP II structure are linked via a simple power-law scaling relationship, which was tested using the experimental R h of 22 IDPs covering a wide range of peptide lengths, net charge, and sequence composition. Charge effects on R h were found to be generally weak when compared to PP II effects on R h. Results from this study indicate that the hydrodynamic dimensions of IDPs are evidence of considerable sequence-dependent backbone propensities for PP II structure that qualitatively, if not quantitatively, match conformational propensities measured in peptides. PMID:26727467

Using Quality Attributes to Bridge Systems Engineering Gaps : A Juno Ground Data Systems Case Study

NASA Technical Reports Server (NTRS)

Dubon, Lydia P.; Jackson, Maddalena M.; Thornton, Marla S.

2012-01-01

The Juno Mission to Jupiter is the second mission selected by the NASA New Frontiers Program. Juno launched August 2011 and will reach Jupiter July 2016. Juno's payload system is composed of nine instruments plus a gravity science experiment. One of the primary functions of the Juno Ground Data System (GDS) is the assembly and distribution of the CFDP (CCSDS File Delivery Protocol) product telemetry, also referred to as raw science data, for eight out of the nine instruments. The GDS accomplishes this with the Instrument Data Pipeline (IDP). During payload integration, the first attempt to exercise the IDP in a flight like manner revealed that although the functional requirements were well understood, the system was unable to meet latency requirements with the as-is heritage design. A systems engineering gap emerged between Juno instrument data delivery requirements and the assumptions behind the heritage flight-ground interactions. This paper describes the use of quality attributes to measure and overcome this gap by introducing a new systems engineering activity, and a new monitoring service architecture that successfully delivered the performance metrics needed to validate Juno IDP.

Applying Thiessen Polygon Catchment Areas and Gridded Population Weights to Estimate Conflict-Driven Population Changes in South Sudan

NASA Astrophysics Data System (ADS)

Jordan, L.

2017-10-01

Recent violence in South Sudan produced significant levels of conflict-driven migration undermining the accuracy and utility of both national and local level population forecasts commonly used in demographic estimates, public health metrics and food security proxies. This article explores the use of Thiessen Polygons and population grids (Gridded Population of the World, WorldPop and LandScan) as weights for estimating the catchment areas for settlement locations that serve large populations of internally displaced persons (IDP), in order to estimate the county-level in- and out-migration attributable to conflict-driven displacement between 2014-2015. Acknowledging IDP totals improves internal population estimates presented by global population databases. Unlike other forecasts, which produce spatially uniform increases in population, accounting for displaced population reveals that 15 percent of counties (n = 12) increased in population over 20 percent, and 30 percent of counties (n = 24) experienced zero or declining population growth, due to internal displacement and refugee out-migration. Adopting Thiessen Polygon catchment zones for internal migration estimation can be applied to other areas with United Nations IDP settlement data, such as Yemen, Somalia, and Nigeria.

Coordinated Stem and NanoSIMS Analysis of Enstatite Whiskers in Interplanetary Dust Particles

NASA Technical Reports Server (NTRS)

Nakamura-Messenger, K.; Messenger, Scott; Keller, L. P.

2009-01-01

Enstatite whiskers (less than 10 micrometer length, less than 200 nanometer width) occur in chondritic-porous interplanetary dust particles (CP IDPs), an Antarctic micrometeorite and a comet 81P/Wild-2 sample. The whiskers are typically elongated along the [100] axis and contain axial screw dislocations, while those in terrestrial rocks and meteorites are elongated along [001]. The unique crystal morphologies and microstructures are consistent with the enstatite whiskers condensing above approximately 1300 K in a low-pressure nebular or circumstellar gas. To constrain the site of enstatite whisker formation, we carried out coordinated mineralogical, chemical and oxygen isotope measurements on enstatite whiskers in a CP IDP.

Goulds Belt, Interstellar Clouds, and the Eocene Oligocene Helium-3 Enhancement

NASA Technical Reports Server (NTRS)

Rubincam, David Parry

2015-01-01

Drag from hydrogen in the interstellar cloud which formed Gould's Belt may have sent interplanetary dust particle (IDPs) and small meteoroids with embedded helium to the Earth, perhaps explaining part the helium-3 flux increase seen in the sedimentary record near the Eocene-Oligocene transition. Assuming the Solar System passed through part of the cloud, IDPs in the inner Solar System may have been dragged to Earth, while dust and small meteoroids in the asteroid belt up to centimeter size may have been dragged to the resonances, where their orbital eccentricities were pumped up into Earth-crossing orbits; however, this hypotheses does not explain the Popigai and Chesapeake Bay impacts.

NASA's future Earth observation plans

NASA Astrophysics Data System (ADS)

Neeck, Steven P.; Paules, Granville E.; McCuistion Ramesh, J. D.

2004-11-01

NASA's Science Mission Directorate, working with its domestic and international partners, provides accurate, objective scientific data and analysis to advance our understanding of Earth system processes. Learning more about these processes will enable improved prediction capability for climate, weather, and natural hazards. Earth interactions occur on a continuum of spatial and temporal scales ranging from short-term weather to long-term climate, and from local and regional to global. Quantitatively describing these changes means precisely measuring from space scores of biological and geophysical parameters globally. New missions that SMD will launch in the coming decade will complement the first series of the Earth Observing System. These next generation systematic measurement missions are being planned to extend or enhance the record of science-quality data necessary for understanding and predicting global change. These missions include the NPOESS Preparatory Project, Ocean Surface Topography Mission, Global Precipitation Measurement, Landsat Data Continuity Mission, and an aerosol polarimetry mission called Glory. New small explorer missions will make first of a kind Earth observations. The Orbiting Carbon Observatory will measure sources and sinks of carbon to help the Nation and the world formulate effective strategies to constrain the amount of this greenhouse gas in the atmosphere. Aquarius will measure ocean surface salinity which is key to ocean circulation in the North Atlantic that produces the current era's mild climate in northern Europe. HYDROS will measure soil moisture globally. Soil moisture is critical to agriculture and to managing fresh water resources. NASA continues to design, develop and launch the Nation's civilian operational environmental satellites, in both polar and geostationary orbits, by agreement with the National Oceanic and Atmospheric Administration (NOAA). NASA plans to develop an advanced atmospheric sounder, GIFTS, for

Soft X-Ray Photoionizing Organic Matter from Comet Wild 2: Evidence for the Production of Organic Matter by Impact Processes

NASA Technical Reports Server (NTRS)

Zolensky, Michael E.; Wirick, S.; Flynn, G. J.; Jacobsen, C.; Na

2011-01-01

The Stardust mission collected both mineral and organic matter from Comet Wild 2 [1,2,3,4]. The organic matter discovered in Comet Wild 2 ranges from aromatic hydrocarbons to simple aliphatic chains and is as diverse and complex as organic matter found in carbonaceous chondrites and interplanetary dust particles.[3,5,6,7,8,9]. Compared to insoluble organic matter from carbonaceous chondrites the organic matter in Comet Wild 2 more closely resembles organic matter found in the IDPS both hydrous and anhydrous. Common processes for the formation of organic matter in space include: Fischer-Tropsch, included with this aqueous large body and moderate heating alterations; UV irradiation of ices; and; plasma formation and collisions. The Fischer-Tropsch could only occur on large bodies processes, and the production of organic matter by UV radiation is limited by the penetration depth of UV photons, on the order of a few microns or less for most organic matter, so once organic matter coats the ices it is formed from, the organic production process would stop. Also, the organic matter formed by UV irradiation would, by the nature of the process, be in-sensitive to photodissocation from UV light. The energy of soft X-rays, 280-300 eV occur within the range of extreme ultraviolet photons. During the preliminary examination period we found a particle that nearly completely photoionized when exposed to photons in the energy range 280-310eV. This particle experienced a long exposure time to the soft x-ray beam which caused almost complete mass loss so little chemical information was obtain. During the analysis of our second allocation we have discovered another particle that photoionized at these energies but the exposure time was limited and more chemical information was obtained.

Improved Thermal-Vacuum Compatible Flat Plate Radiometric Souce for System-Level Testing of Optical Sensors

NASA Technical Reports Server (NTRS)

Schwarz, Mark A.; Kent, Craig J.; Bousquet, Robert; Brown, Steven W.

2015-01-01

This work describes the development of an improved vacuum compatible flat plate radiometric source used for characterizing and calibrating remote optical sensors, in situ, throughout their testing period. The original flat plate radiometric source was developed for use by the VIIRS instrument during the NPOESS Preparatory Project (NPP). Following this effort, the FPI has had significant upgrades in order to improve both the radiometric throughput and uniformity. Results of the VIIRS testing with the reconfigured FPI are reported and discussed.

1D-VAR Retrieval Using Superchannels

NASA Technical Reports Server (NTRS)

Liu, Xu; Zhou, Daniel; Larar, Allen; Smith, William L.; Schluessel, Peter; Mango, Stephen; SaintGermain, Karen

2008-01-01

Since modern ultra-spectral remote sensors have thousands of channels, it is difficult to include all of them in a 1D-var retrieval system. We will describe a physical inversion algorithm, which includes all available channels for the atmospheric temperature, moisture, cloud, and surface parameter retrievals. Both the forward model and the inversion algorithm compress the channel radiances into super channels. These super channels are obtained by projecting the radiance spectra onto a set of pre-calculated eigenvectors. The forward model provides both super channel properties and jacobian in EOF space directly. For ultra-spectral sensors such as Infrared Atmospheric Sounding Interferometer (IASI) and the NPOESS Airborne Sounder Testbed Interferometer (NAST), a compression ratio of more than 80 can be achieved, leading to a significant reduction in computations involved in an inversion process. Results will be shown applying the algorithm to real IASI and NAST data.

Interstellar and Solar Nebula Materials in Cometary Dust

NASA Technical Reports Server (NTRS)

Messenger, Scott; Nakamura-Messenger, Keiko; Keller, Lindsay; Nguyen, Ann; Clemett, Simon

2017-01-01

Laboratory studies of cometary dust collected in the stratosphere and returned from comet 81P/Wild 2 by the Stardust spacecraft have revealed ancient interstellar grains and molecular cloud organic matter that record a range of astrophysical processes and the first steps of planetary formation. Presolar materials are rarer meteorites owing to high temperature processing in the solar nebula and hydrothermal alteration on their asteroidal parent bodies. The greater preservation of presolar materials in comets is attributed to their low accretion temperatures and limited planetary processing. Yet, comets also contain a large complement of high temperature materials from the inner Solar System. Owing to the limited and biased sampling of comets to date, the proportions of interstellar and Solar System materials within them remains highly uncertain. Interstellar materials are identified by coordinated isotopic, mineralogical, and chemical measurements at the scale of individual grains. Chondritic porous interplanetary dust particles (CP IDPs) that likely derive from comets are made up of 0.1 - 10 micron-sized silicates, Fe-Ni-sulfides, oxides, and other phases bound by organic material. As much as 1% of the silicates are interstellar grains that have exotic isotopic compositions imparted by nucleosynthetic processes in their parent stars. Crystalline silicates in CP IDPs dominantly have normal isotopic compositions and probably formed in the Solar System. 81P samples include isotopically normal refractory minerals that resemble Ca-Al rich inclusions and chondrules common in meteorites. The origins of sub-micron amorphous silicates in IDPs are not certain, but at least a few % of them are interstellar grains. The remainder have isotopic compositions consistent with Solar System origins and elemental compositions that are inconsistent with interstellar grain properties, thus favoring formation in the solar nebula [4]. The organic component in comets and primitive

Role of non-native electrostatic interactions in the coupled folding and binding of PUMA with Mcl-1

PubMed Central

Chu, Wen-Ting; Clarke, Jane; Shammas, Sarah L.; Wang, Jin

2017-01-01

PUMA, which belongs to the BH3-only protein family, is an intrinsically disordered protein (IDP). It binds to its cellular partner Mcl-1 through its BH3 motif, which folds upon binding into an α helix. We have applied a structure-based coarse-grained model, with an explicit Debye—Hückel charge model, to probe the importance of electrostatic interactions both in the early and the later stages of this model coupled folding and binding process. This model was carefully calibrated with the experimental data on helical content and affinity, and shown to be consistent with previously published experimental data on binding rate changes with respect to ionic strength. We find that intramolecular electrostatic interactions influence the unbound states of PUMA only marginally. Our results further suggest that intermolecular electrostatic interactions, and in particular non-native electrostatic interactions, are involved in formation of the initial encounter complex. We are able to reveal the binding mechanism in more detail than is possible using experimental data alone however, and in particular we uncover the role of non-native electrostatic interactions. We highlight the potential importance of such electrostatic interactions for describing the binding reactions of IDPs. Such approaches could be used to provide predictions for the results of mutational studies. PMID:28369057

Chemically anomalous, pre-accretionally irradiated grains in interplanetary dust -- interstellar grains?. [Abstract only

NASA Technical Reports Server (NTRS)

Bradley, J. P.

1994-01-01

Ultrafine-grained matrix is a unique and fundamental building block of chondritic porous (CP) interplanetary dust particles. Most IDPs so far determined to be of cometary origin belong to the CP class. The matrix in CP IDPs is not homogeneous but rather a loose mixture of discrete single crystals (e.g., olivine, pyroxene, Fe sulfides) and polyphase grains. The petrographic diversity observed among the polyphase grains suggest that they were formed under variable physiochemical conditions. One particular class of polyphase grains are a dominant component in cometary IDPs. Although their occurrence is well documented, the terminology used to describe them is confused. They have been called many names. Here they are simply called GEMS (Glass with Embedded Metal and Sulfides). The bulk compositions of GEMS are within a factor of 3 chondritic (solar) for all major elements except C. Quantitative thin-film X-ray (EDS) analyses have shown that GEMS are systematically depleted in Mg and Si, enriched in S, Fe, and Ni, and stoichiometrically enriched in O. Electron energy-loss spectroscopy (EELS) suggests that the excess O is present as hydroxyl (-OH) groups. These same chemical 'anomalies' were observed in solar-wind-irradiated amorphous rims on the surfaces of IDPs, suggesting that the compositions of GEMS reflect prior exposure to ionizing radiation. In order to test this hypothesis, a sample of Allende (CV3) matrix was exposed to proton flux. Radiation-damaged amorphous rims on olivine and pyroxene crystals in the Allende sample were found to be depleted in Mg and Ca, enriched in S, Fe, and Ni, and stoichiometrically enriched in O. Thus, the compositions of GEMS are indeed consistent with exposure to ionizing radiation. This study suggests that chemical as well as isotopic anomalies may be used to identify presolar interstellar grains in primitive meteoritic materials.

Binding Rate Constants Reveal Distinct Features of Disordered Protein Domains.

PubMed

Dogan, Jakob; Jonasson, Josefin; Andersson, Eva; Jemth, Per

2015-08-04

Intrinsically disordered proteins (IDPs) are abundant in the proteome and involved in key cellular functions. However, experimental data about the binding kinetics of IDPs as a function of different environmental conditions are scarce. We have performed an extensive characterization of the ionic strength dependence of the interaction between the molten globular nuclear co-activator binding domain (NCBD) of CREB binding protein and five different protein ligands, including the intrinsically disordered activation domain of p160 transcriptional co-activators (SRC1, TIF2, ACTR), the p53 transactivation domain, and the folded pointed domain (PNT) of transcription factor ETS-2. Direct comparisons of the binding rate constants under identical conditions show that the association rate constant, kon, for interactions between NCBD and disordered protein domains is high at low salt concentrations (90-350 × 10(6) M(-1) s(-1) at 4 °C) but is reduced significantly (10-30-fold) with an increasing ionic strength and reaches a plateau around physiological ionic strength. In contrast, the kon for the interaction between NCBD and the folded PNT domain is only 7 × 10(6) M(-1) s(-1) (4 °C and low salt) and displays weak ionic strength dependence, which could reflect a distinctly different association that relies less on electrostatic interactions. Furthermore, the basal rate constant (in the absence of electrostatic interactions) is high for the NCBD interactions, exceeding those typically observed for folded proteins. One likely interpretation is that disordered proteins have a large number of possible collisions leading to a productive on-pathway encounter complex, while folded proteins are more restricted in terms of orientation. Our results highlight the importance of electrostatic interactions in binding involving IDPs and emphasize the significance of including ionic strength as a factor in studies that compare the binding properties of IDPs to those of ordered proteins.

Fossil diatoms imply common cometary origin of space-dust and the Polonnaruwa meteorite

NASA Astrophysics Data System (ADS)

Miyake, N.; Wallis, M. K.; Wickramasinghe, N. C.

2013-09-01

IDPs collected in 2001 at 40km altitude by cryosamplers studied via scanning electron microscopy and EDX were found to contain siliceous fibres and whiskers, some isolated but often embedded in a mineral matrix. The newly-arrived Polonnaruwa meteorite gives strong evidence for the hypothesis that they are fragments of diatoms agglomerating on solar system icy bodies. Diatom frustules and even whole diatom skeletons are identifiable within the meteorite. Specimens of a siliceous exoskeleton with multiple spines/whiskers have also been found, thought to be freshwater diatoms. As diatoms are dependent on a source of nitrogenous organics, the siliceous whiskers within IDPs would be an indicator of a photosynthesizing ecosystem, probably on a comet.

Nuclear Weapon Effect Research at PSR (Pacific-Sierra Research Corporation) - 1983. Acute Radiation Effects on Individual Crewmember Performance

DTIC Science & Technology

1984-08-31

information and data from other IDP investigations. Along with the IDP Core Group, DNA staff members David L. Auton and Robert W . Young of Science and...questijonna ire for tli.i s p ilIot riiii wals a moSt ident ica I to tihe f inal version * w ith a few di fterences in I nu ige an normal tIinme va ti...46J a C) C 7 r- A *.- . -4 0 - W - 24AJC a) -~ý 4 -4 m’.*4 W ~C V- CL CC Cý C) Z, Q) > :J U.- .0 M 0 -0t4. )v. , (1) wl a)r 0 )-4 1 QC ) f O * - 1J) a

Organics Captured from Comet Wild 2 by the Stardust Spacecraft

NASA Technical Reports Server (NTRS)

Sandford, Scott A.; Aleon, Jerome; Araki, Tohru; Bajt, Sasa; Baratta, Giuseppe A.; Borg, Janet; Brucato, John R.; Burchell, Mark J.; Busemann, Henner; Butterworth, Anna;

Effects of correcting in situ ruminal microbial colonization of feed particles on the relationship between ruminally undegraded and intestinally digested crude protein in concentrate feeds.

PubMed

González, Javier; Mouhbi, Rabiaa; Guevara-González, Jesús Alberto; Arroyo, José María

2018-02-01

In situ estimates of ruminally undegraded protein (RUP) and intestinally digested protein (IDP) of ten concentrates, uncorrected or corrected for the ruminal microbial colonization, were used to examine the effects of this correction on the relationship between IDP and RUP values. Both variables were established for three rumen and duodenum cannulated wethers using 15 N labeling-techniques and considering measured rates of ruminal particle comminution (k c ) and outflow (k p ). A covariance analysis showed that the close relationship found between both variables (IDP = -0.0132 ± 0.00679 + 0.776 ± 0.0002 RUP; n = 60; P < 0.001; r = 0.960) is not affected by correcting for microbial colonization (P = 0.682). The IDP content in concentrates and industrial by-products can be predicted from RUP values, thus avoiding the laborious and complex procedure of determining intestinal digestibility; however, a larger sample of feeds is necessary to achieve more accurate predictions. The lack of influence of the correction for microbial contamination on the prediction observed in the present study increases the data available for this prediction. However, only the use of corrected values may provide an accurate evaluation. © 2017 Society of Chemical Industry. © 2017 Society of Chemical Industry.

DOE Office of Scientific and Technical Information (OSTI.GOV)

McAfee, J.G.; Krauss, D.J.; Subramanian, G.

The 3-hour biodistribution of /sup 99m/Tc complexes of five diphosphonates (HMDP, NMMDP, DMAD, DPD, and APD), imidodiphosphonate (IDP), and pyrophosphate (PYP) was compared in rats with segmental renal infarction induced by a 1-hour occlusion of a renal artery branch. /sup 95m/Tc labeled MDP was a reference substance in all animals. Three agents (APD, HMDP and IDP) had a higher infarct/normal kidney concentration ratio than MDP, the latter two by virtue of a lower content in normal kidney. HMDP, DPD, and IDP had very high liver concentrations. DPD showed relatively high concentrations in soft tissues and blood. The blood and kidneymore » levels of PYP were higher than those of MDP but the infarct/normal kidney ratios were similar. None of the agents had a higher uptake in bone than MDP: four had a significantly lower uptake. The increased concentration of /sup 99m/Tc MDP in the infarcts was readily seen in camera images one day after renal artery occlusion, but not at three or seven days. Increased diphosphonate uptake was accompanied by an influx of calcium in both cortex and medulla. The accumulation of diphosphonate in areas of infarction was not modified by infusions of verapamil or Captopril.« less

Spatiotemporal Co-existence of Female Thyroid and Breast Cancers in Hangzhou, China

NASA Astrophysics Data System (ADS)

Fei, Xufeng; Christakos, George; Lou, Zhaohan; Ren, Yanjun; Liu, Qingmin; Wu, Jiaping

2016-06-01

Thyroid and breast cancers (TC, BC) are common female malignant tumors worldwide. Studies suggest that TC patients have a higher BC risk, and vice versa. However, it has not been investigated quantitatively if there is an association between the space-time TC and BC incidence distributions at the population level. This work aims to answer this question. 5358 TC and 8784 BC (female) cases were diagnosed in Hangzhou (China, 2008-2012). Pearson and Spearman rank correlation coefficients of the TC and BC incidences were high, and their patterns were geographically similar. The spatiotemporal co-existence of TC and BC distributions was investigated using the integrative disease predictability (IDP) criterion: if TC-BC association is part of the disease mapping knowledge bases, it should yield improved space-time incidence predictions. Improved TC (BC) incidence predictions were generated when integrating both TC and BC data than when using only TC (BC) data. IDP consistently demonstrated the spatiotemporal co-existence of TC and BC distributions throughout Hangzhou (2008-2012), which means that when the population experiences high incidences of one kind of cancer attention should be paid to the other kind of cancer too. The strength of TC-BC association was measured by the IDP coefficients and incidence prediction accuracy.

Comparison of Carbon XANES Spectra from an Iron Sulfide from Comet Wild 2 with an Iron Sulfide Interplanetary Dust Particle

NASA Technical Reports Server (NTRS)

Wirick, S.; Flynn, G. J.; Keller, L. P.; Sanford, S. A.; Zolensky, M. E.; Messenger, Nakamura K.; Jacobsen, C.

2008-01-01

Among one of the first particles removed from the aerogel collector from the Stardust sample return mission was an approx. 5 micron sized iron sulfide. The majority of the spectra from 5 different sections of this particle suggests the presence of aliphatic compounds. Due to the heat of capture in the aerogel we initially assumed these aliphatic compounds were not cometary but after comparing these results to a heated iron sulfide interplanetary dust particle (IDP) we believe our initial interpretation of these spectra was not correct. It has been suggested that ice coating on iron sulfides leads to aqueous alteration in IDP clusters which can then lead to the formation of complex organic compounds from unprocessed organics in the IDPs similar to unprocessed organics found in comets [1]. Iron sulfides have been demonstrated to not only transform halogenated aliphatic hydrocarbons but also enhance the bonding of rubber to steel [2,3]. Bromfield and Coville (1997) demonstrated using Xray photoelectron spectroscopy that "the surface enhancement of segregated sulfur to the surface of sulfided precipitated iron catalysts facilitates the formation of a low-dimensional structure of extraordinary properties" [4]. It may be that the iron sulfide acts in some way to protect aliphatic compounds from alteration due to heat.

Advantages of synchrotron radiation circular dichroism spectroscopy to study intrinsically disordered proteins.

PubMed

Kumagai, Patricia S; DeMarco, Ricardo; Lopes, Jose L S

2017-10-01

The unordered secondary structural content of an intrinsically disordered protein (IDP) is susceptible to conformational changes induced by many different external factors, such as the presence of organic solvents, removal of water, changes in temperature, binding to partner molecules, and interaction with lipids and/or other ligands. In order to characterize the high-flexibility nature of an IDP, circular dichroism (CD) spectroscopy is a particularly useful method due to its capability of monitoring both subtle and remarkable changes in different environments, relative ease in obtaining measurements, the small amount of sample required, and the capability for sample recovery (sample not damaged) and others. Using synchrotron radiation as the light source for CD spectroscopy represents the state-of-the-art version of this technique with feasibility of accessing the lower wavelength UV region, and therefore presenting a series of advantages over conventional circular dichroism (cCD) to monitor a protein conformational behavior, check protein stability, detect ligand binding, and many others. In this paper, we have performed a comparative study using cCD and SRCD methods for investigating the secondary structure and the conformational behavior of natively unfolded proteins: MEG-14 and soybean trypsin inhibitor. We show that the SRCD technique greatly improves the analysis and accuracy of the studies on the conformations of IDPs.

Spatiotemporal Co-existence of Female Thyroid and Breast Cancers in Hangzhou, China

PubMed Central

Fei, Xufeng; Christakos, George; Lou, Zhaohan; Ren, Yanjun; Liu, Qingmin; Wu, Jiaping

2016-01-01

Thyroid and breast cancers (TC, BC) are common female malignant tumors worldwide. Studies suggest that TC patients have a higher BC risk, and vice versa. However, it has not been investigated quantitatively if there is an association between the space-time TC and BC incidence distributions at the population level. This work aims to answer this question. 5358 TC and 8784 BC (female) cases were diagnosed in Hangzhou (China, 2008–2012). Pearson and Spearman rank correlation coefficients of the TC and BC incidences were high, and their patterns were geographically similar. The spatiotemporal co-existence of TC and BC distributions was investigated using the integrative disease predictability (IDP) criterion: if TC-BC association is part of the disease mapping knowledge bases, it should yield improved space-time incidence predictions. Improved TC (BC) incidence predictions were generated when integrating both TC and BC data than when using only TC (BC) data. IDP consistently demonstrated the spatiotemporal co-existence of TC and BC distributions throughout Hangzhou (2008–2012), which means that when the population experiences high incidences of one kind of cancer attention should be paid to the other kind of cancer too. The strength of TC-BC association was measured by the IDP coefficients and incidence prediction accuracy. PMID:27341638

Intrinsically Disordered Energy Landscapes

PubMed Central

Chebaro, Yassmine; Ballard, Andrew J.; Chakraborty, Debayan; Wales, David J.

2015-01-01

Analysis of an intrinsically disordered protein (IDP) reveals an underlying multifunnel structure for the energy landscape. We suggest that such ‘intrinsically disordered’ landscapes, with a number of very different competing low-energy structures, are likely to characterise IDPs, and provide a useful way to address their properties. In particular, IDPs are present in many cellular protein interaction networks, and several questions arise regarding how they bind to partners. Are conformations resembling the bound structure selected for binding, or does further folding occur on binding the partner in a induced-fit fashion? We focus on the p53 upregulated modulator of apoptosis (PUMA) protein, which adopts an -helical conformation when bound to its partner, and is involved in the activation of apoptosis. Recent experimental evidence shows that folding is not necessary for binding, and supports an induced-fit mechanism. Using a variety of computational approaches we deduce the molecular mechanism behind the instability of the PUMA peptide as a helix in isolation. We find significant barriers between partially folded states and the helix. Our results show that the favoured conformations are molten-globule like, stabilised by charged and hydrophobic contacts, with structures resembling the bound state relatively unpopulated in equilibrium. PMID:25999294

pE-DB: a database of structural ensembles of intrinsically disordered and of unfolded proteins.

PubMed

Varadi, Mihaly; Kosol, Simone; Lebrun, Pierre; Valentini, Erica; Blackledge, Martin; Dunker, A Keith; Felli, Isabella C; Forman-Kay, Julie D; Kriwacki, Richard W; Pierattelli, Roberta; Sussman, Joel; Svergun, Dmitri I; Uversky, Vladimir N; Vendruscolo, Michele; Wishart, David; Wright, Peter E; Tompa, Peter

2014-01-01

The goal of pE-DB (http://pedb.vib.be) is to serve as an openly accessible database for the deposition of structural ensembles of intrinsically disordered proteins (IDPs) and of denatured proteins based on nuclear magnetic resonance spectroscopy, small-angle X-ray scattering and other data measured in solution. Owing to the inherent flexibility of IDPs, solution techniques are particularly appropriate for characterizing their biophysical properties, and structural ensembles in agreement with these data provide a convenient tool for describing the underlying conformational sampling. Database entries consist of (i) primary experimental data with descriptions of the acquisition methods and algorithms used for the ensemble calculations, and (ii) the structural ensembles consistent with these data, provided as a set of models in a Protein Data Bank format. PE-DB is open for submissions from the community, and is intended as a forum for disseminating the structural ensembles and the methodologies used to generate them. While the need to represent the IDP structures is clear, methods for determining and evaluating the structural ensembles are still evolving. The availability of the pE-DB database is expected to promote the development of new modeling methods and leads to a better understanding of how function arises from disordered states.

Initial Retrieval Validation from the Joint Airborne IASI Validation Experiment (JAIVEx)

NASA Technical Reports Server (NTRS)

Zhou, Daniel K.; Liu, Xu; Smith, WIlliam L.; Larar, Allen M.; Taylor, Jonathan P.; Revercomb, Henry E.; Mango, Stephen A.; Schluessel, Peter; Calbet, Xavier

2007-01-01

The Joint Airborne IASI Validation Experiment (JAIVEx) was conducted during April 2007 mainly for validation of the Infrared Atmospheric Sounding Interferometer (IASI) on the MetOp satellite, but also included a strong component focusing on validation of the Atmospheric InfraRed Sounder (AIRS) aboard the AQUA satellite. The cross validation of IASI and AIRS is important for the joint use of their data in the global Numerical Weather Prediction process. Initial inter-comparisons of geophysical products have been conducted from different aspects, such as using different measurements from airborne ultraspectral Fourier transform spectrometers (specifically, the NPOESS Airborne Sounder Testbed Interferometer (NAST-I) and the Scanning-High resolution Interferometer Sounder (S-HIS) aboard the NASA WB-57 aircraft), UK Facility for Airborne Atmospheric Measurements (FAAM) BAe146-301 aircraft insitu instruments, dedicated dropsondes, radiosondes, and ground based Raman Lidar. An overview of the JAIVEx retrieval validation plan and some initial results of this field campaign are presented.

NPP After Launch: Characterizing ATMS Performance

NASA Technical Reports Server (NTRS)

Lambrigtsen, Bjorn

2011-01-01

The NPOESS Preparatory Project (NPP) mission is scheduled to launch in the fall of 2011. Although several teams from the government and the instrument contractor will be assessing and characterizing the performance of the Advanced Technology Microwave Sounder (ATMS) and the Cross-track Infrared Sounder (CrIS) sounding suite, the NASA NPP Science Team will be paying particular attention to the aspects of these sensors that affect their utility for atmospheric and climate research. In this talk we discuss relevant aspects of ATMS and our post launch analysis approach.

Everyone Wins: A Mars-Impact Origin for Carbonaceous Phobos and Deimos

NASA Technical Reports Server (NTRS)

Fries, M.; Welzenbach, L.; Steele, A.

2016-01-01

Discussions of Phobos' and Deimos' origin(s) tend to feature an orthogonally opposed pair of observations: dynamical studies which favor coalescence of the moons from an orbital debris ring arising from a large impact on Mars; and reflectance spectroscopy of the moons that indicate a carbonaceous composition that is not consistent with Martian surface materials. One way to reconcile this discrepancy is to consider the option of a Mars-impact origin for Phobos and Deimos, followed by surficial decoration of carbon-rich materials by interplanetary dust particles (IDP). The moons experience a high IDP flux because of their location in Mars' gravity well. Calculations show that accreted carbon is sufficient to produce a surface with reflectance spectra resembling carbonaceous chondrites.

Multielement analysis of interplanetary dust particles using TOF-SIMS

NASA Technical Reports Server (NTRS)

Stephan, T.; Kloeck, W.; Jessberger, E. K.; Rulle, H.; Zehnpfenning, J.

1993-01-01

Sections of three stratospheric particles (U2015G1, W7029*A27, and L2005P9) were analyzed with TOF-SIMS (Time Of Flight-Secondary Ion Mass Spectrometry) continuing our efforts to investigate the element distribution in interplanetary dust particles (IDP's) with high lateral resolution (approximately 0.2 micron), to examine possible atmospheric contamination effects, and to further explore the abilities of this technique for element analysis of small samples. The samples, previously investigated with SXRF (synchrotron X-ray fluorescence analysis), are highly enriched in Br (Br/Fe: 59 x CI, 9.2 x CI, and 116 x CI, respectively). U2015G1 is the IDP with the by far highest Zn/Fe-ratio (81 x CI) ever reported in chondritic particles.

Coordinated Analyses of Mineral-organic Matter Associations in Interplanetary Dust Particles

NASA Technical Reports Server (NTRS)

Nakamura-Messenger, K.; Herzog, G. F.; Smith, T.; Keller, L. P.; Flynn, G. J.; Khodja, H.; Taylor, S.; Wirick, S.; Messenger, S.

2012-01-01

Little is known about the timing and processes involved in the incorporation of organic matter with inorganic materials in early Solar System bodies. Recently, X-ray absorption near-edge spectroscopy (XANES) studies showed carbon-rich rims surrounding individual mineral grains in anhydrous IDPs [1,2]. These carbonaceous rims are believed to have formed prior to parent body formation and likely served to bond mineral grains during accretion into larger aggregates. We are exploring the nature of these carbonaceous rims through coordinated analyses of their chemistry, mineralogy, spectroscopy and isotopic characteristics. Here we report our preliminary mineralogical observations.

Hydrocarbons on Saturns Satellites: Relationship to Interstellar Dust and the Solar Nebula

NASA Technical Reports Server (NTRS)

Cruikshank, D. P.

2012-01-01

To understand the origin and evolution of our Solar System, and the basic components that led to life on Earth, we study interstellar and planetary spectroscopic signatures. The possible relationship of organic material detected in carbonaceous meteorites, interplanetary dust particles (IDPs), comets and the interstellar medium have been the source of speculation over the years as the composition and processes that governed the early solar nebula have been explored to understand the extent to which primitive material survived or became processed. The Cassini VIMS has provided new data relevant to this problem. Three of Saturn's satellites, Phoebe, Iapetus, and Hyperion, are found to have aromatic and aliphatic hydrocarbons on their surfaces. The aromatic hydrocarbon signature (C-H stretching mode at 3.28 micrometers) is proportionally significantly stronger (relative to the aliphatic bands) than that seen in other Solar System bodies (e.g., comets) and materials (Stardust samples, IDPs, meteorites) and the distinctive sub-features of the 3.4 micrometer aliphatic band (CH2 and CH3 groups) are reminiscent of those widely detected throughout the diffuse ISM. Phoebe may be a captured object that originated in the region beyond the present orbit of Neptune, where the solar nebula contained a large fraction of original interstellar ice and dust that was less processed than material closer to the Sun. Debris from Phoebe now resident on Iapetus and Hyperion, as well as o Phoebe itself, thus presents a unique blend of hydrocarbons, amenable to comparisons with interstellar hydrocarbons and other Solar System materials. The dust ring surrounding Saturn, in which Phoebe is embedded, probably originated from a collision with Phoebe. Dust ring particles are the likely source of the organic-bearing materials, and perhaps the recently identified small particles of Fe detected on Saturn's satellites. Lab measurements of the absolute band strengths of representative aliphatic and

The Mineralogy of Circumstellar Silicates Preserved in Cometary Dust

NASA Technical Reports Server (NTRS)

Keller, L. P.; Messenger, S.

2010-01-01

Interplanetary dust particles (IDPs) contain a record of the building blocks of the solar system including presolar grains, molecular cloud material, and materials formed in the early solar nebula. Cometary IDPs have remained relatively unaltered since their accretion because of the lack of parent body thermal and aqueous alteration. We are using coordinated transmission electron microscope (TEM) and ion microprobe studies to establish the origins of the various components within cometary IDPs. Of particular interest is the nature and abundance of presolar silicates in these particles because astronomical observations suggest that crystalline and amorphous silicates are the dominant grain types produced in young main sequence stars and evolved O-rich stars. Five circumstellar grains have been identified including three amorphous silicate grains and two polycrystalline aggregates. All of these grains are between 0.2 and 0.5 micrometers in size. The isotopic compositions of all five presolar silicate grains fall within the range of presolar oxides and silicates, having large (17)O-enrichments and normal (18)O/(16)O ratios (Group 1 grains from AGB and RG stars). The amorphous silicates are chemically heterogeneous and contain nanophase FeNi metal and FeS grains in a Mg-silicate matrix. Two of the amorphous silicate grains are aggregates with subgrains showing variable Mg/Si ratios in chemical maps. The polycrystalline grains show annealed textures (equilibrium grains boundaries, uniform Mg/Fe ratios), and consist of 50-100 nm enstatite and pyrrhotite grains with lesser forsterite. One of the polycrystalline aggregates contains a subgrain of diopside. The polycrystalline aggregates form by subsolidus annealing of amorphous precursors. The bulk compositions of the five grains span a wide range in Mg/Si ratios from 0.4 to 1.2 (avg. 0.86). The average Fe/Si (0.40) and S/Si (0.21) ratios show a much narrower range of values and are approximately 50% of their solar

Ensemble characterization of an intrinsically disordered FG-Nup peptide and its F>A mutant in DMSO-d6.

PubMed

Reid, Korey M; Sunanda, Punnepalli; Raghothama, S; Krishnan, V V

2017-11-01

Intrinsically disordered proteins (IDP) lack a well-defined 3D-structure under physiological conditions, yet, the inherent disorder represented by an ensemble of conformation plays a critical role in many cellular and regulatory processes. Nucleoporins, or Nups, are the proteins found in the nuclear pore complex (NPC). The central pore of the NPC is occupied by Nups, which have phenylalanine-glycine domain repeats and are intrinsically disordered, and therefore are termed FG-Nups. These FG-domain repeats exhibit differing cohesiveness character and differ from least (FG) to most (GLFG) cohesive. The designed FG-Nup is a 25 AA model peptide containing a noncohesive FG-motif flanked by two cohesive GLFG-motifs (WT peptide). Complete NMR-based ensemble characterization of this peptide along with a control peptide with an F>A substitution (MU peptide) are discussed. Ensemble characterization of the NMR-determined models suggests that both the peptides do not have consistent secondary structures and continue to be disordered. Nonetheless, the role of cohesive elements mediated by the GLFG motifs is evident in the WT ensemble of structures that are more compact than the MU peptide. The approach presented here allows an alternate way to investigate the specific roles of distinct amino acid motifs that translate into the long-range organization of the ensemble of structures and in general on the nature of IDPs. © 2017 Wiley Periodicals, Inc.

Porosity and Permeability of Chondritic Materials

NASA Technical Reports Server (NTRS)

Zolensky, Michael E.; Corrigan, Catherine M.; Dahl, Jason; Long, Michael

1996-01-01

We have investigated the porosity of a large number of chondritic interplanetary dust particles and meteorites by three techniques: standard liquid/gas flow techniques, a new, non-invasive ultrasonic technique, and image processing of backscattered images . The latter technique is obviously best suited to sub-kg sized samples. We have also measured the gas and liquid permeabilities of some chondrites by two techniques: standard liquid/gas flow techniques, and a new, non-destructive pressure release technique. We find that chondritic IDP's have a somewhat bimodal porosity distribution. Peaks are present at 0 and 4% porosity; a tail then extends to 53%. These values suggest IDP bulk densities of 1.1 to 3.3 g/cc. Type 1-3 chondrite matrix porosities range up to 30%, with a peak at 2%. The bulk porosities for type 1-3 chondrites have the same approximate range as exhibited by matrix, indicating that other components of the bulk meteorites (including chondrules and aggregates) have the same average porosity as matrix. These results reveal that the porosity of primitive materials at scales ranging from nanogram to kilogram are similar, implying similar accretion dynamics operated through 12 orders of size magnitude. Permeabilities of the investigated chondrites vary by several orders of magnitude, and there appears to be no simple dependence of permeability with degree of aqueous alteration, or chondrite type.

Detecting Interplanetary Dust Particles with Radars to Study the Dynamics at the Edge of the Space

NASA Technical Reports Server (NTRS)

Janches, Diego

2015-01-01

The Earth's mesosphere is the region of the atmosphere between approximately 60-120 km altitude, where the transition from hydrodynamic flow to molecular diffusion occurs. It is highly dynamic region where turbulence by wave braking is produced and energy is deposited from sources from both, below and above this altitude range. Because aircraft and nearly all balloons reach altitudes below approximately 50 km and orbital spacecrafts are well above approximately 400 km, the mesosphere has only been accessed through the use of sounding rockets or remote sensing techniques, and as a result, it is the most poorly understood part of the atmosphere. In addition, millions of Interplanetary Dust Particles (IDPs) enter the atmosphere. Within the mesosphere most of these IDPs melt or vaporize as a result of collisions with the air particles producing meteors that can be detected with radars. This provides a mean to study the dynamics of this region. In this lecture the basic principles of the utilization of meteor radars to study the dynamics of the mesosphere will be presented. A system overview of these systems will be provided as well as discuss the advantages/disadvantages of these systems, provide details of the data processing methodology and give a brief overview of the current status of the field as well as the vision for the next decade.

Site-Specific Modulation of Charge Controls the Structure and Stimulus Responsiveness of Intrinsically Disordered Peptide Brushes.

PubMed

Bhagawati, Maniraj; Rubashkin, Matt G; Lee, Jessica P; Ananthanarayanan, Badriprasad; Weaver, Valerie M; Kumar, Sanjay

2016-06-14

Intrinsically disordered proteins (IDPs) are an important and emerging class of materials for tailoring biointerfaces. While the importance of chain charge and resultant electrostatic interactions in controlling conformational properties of IDPs is beginning to be explored through in silico approaches, there is a dearth of experimental studies motivated toward a systematic study of these effects. In an effort to explore this relationship, we measured the conformations of two peptides derived from the intrinsically disordered neurofilament (NF) side arm domain: one depicting the wild-type sequence with four lysine-serine-proline repeats (KSP peptide) and another in which the serine residues were replaced with aspartates (KDP peptide), a strategy sometimes used to mimic phosphorylation. Using a variety of biophysical measurements including a novel application of scanning angle interference microscopy, we demonstrate that the KDP peptide assumes comparatively more expanded conformations in solution and forms significantly thicker brushes when immobilized on planar surfaces at high densities. In both settings, the peptides respond to changes in ambient ionic strength, with each peptide showing distinct stimulus-responsive characteristics. While the KDP peptide undergoes compaction with increasing ionic strength as would be expected for a polyampholyte, the KSP peptide shows biphasic behavior, with an initial compaction followed by an expanded state at a higher ionic strength. Together these results support the notion that modulation of charge on IDPs can regulate conformational and interfacial properties.

Needs of Internally Displaced Women and Children in Baghdad, Karbala, and Kirkuk, Iraq

PubMed Central

Lafta, Riyadh; Aflouk, Nesreen A; Dhiaa, Saba; Lyles, Emily; Burnham, Gilbert

2016-01-01

Background: The continuing conflict in Iraq has now created an estimated four million internally displaced persons (IDPs). The bulk of recently displaced persons are in Central Iraq, often in insecure and difficult situations. Objective: To determine the health status and health needs of women and children, age 15 and under, among a sample of this IDP population in Kirkuk, Baghdad, and Karbala governorates. Methods: Data were collected from the senior female in 1216 families which contained 3665 children living in 45 makeshift settlements. Findings: The majority of IDPs were living in tents or religious centers. Repeated displacements were common. Kidnappings were reported by 5.2% of families, and 7.9% of families reported a death of a family member during or after displacement. Intentional violence accounted for 72.3% of deaths. Only a third of children in school at the time of displacement continued in school. On average, households had received assistance on 3.2 occasions since displacement, food being the most common form. Access to health services was difficult. Some form of transport was often required. Few women knew where to secure antenatal services and many did not know where childhood immunization services were available. During or after displacement 307 women had delivered or were currently pregnant. Complications of pregnancies were common, with a quarter reporting anemia, and 22.1% experiencing hemorrhage. Both communicable and non-communicable diseases (NCDs) were common in the women and children in the survey. Scabies, diarrhea and lice were common among children. Among women, hypertension accounted for 36.6% of NCDs and type 2 diabetes for 15.9%. Domestic violence directed against women was reported in 17.4% of families and against children in 26.6% Interpretation: Women and children in IDP settlements of Central Iraq experience many vulnerabilities involving their health, education and their environment, in addition to living in physical danger

Dome C ultracarbonaceous Antarctic micrometeorites. Infrared and Raman fingerprints

NASA Astrophysics Data System (ADS)

Dartois, E.; Engrand, C.; Duprat, J.; Godard, M.; Charon, E.; Delauche, L.; Sandt, C.; Borondics, F.

2018-01-01

Context. UltraCarbonaceous Antarctic MicroMeteorites (UCAMMs) represent a small fraction of interplanetary dust particles reaching the Earth's surface and contain large amounts of an organic component not found elsewhere. They are most probably sampling a contribution from the outer regions of the solar system to the local interplanetary dust particle (IDP) flux. Aims: We characterize UCAMMs composition focusing on the organic matter, and compare the results to the insoluble organic matter (IOM) from primitive meteorites, IDPs, and the Earth. Methods: We acquired synchrotron infrared microspectroscopy (μFTIR) and μRaman spectra of eight UCAMMs from the Concordia/CSNSM collection, as well as N/C atomic ratios determined with an electron microprobe. Results: The spectra are dominated by an organic component with a low aliphatic CH versus aromatic C=C ratio, and a higher nitrogen fraction and lower oxygen fraction compared to carbonaceous chondrites and IDPs. The UCAMMs carbonyl absorption band is in agreement with a ketone or aldehyde functional group. Some of the IR and Raman spectra show a C≡N band corresponding to a nitrile. The absorption band profile from 1400 to 1100 cm-1 is compatible with the presence of C-N bondings in the carbonaceous network, and is spectrally different from that reported in meteorite IOM. We confirm that the silicate-to-carbon content in UCAMMs is well below that reported in IDPs and meteorites. Together with the high nitrogen abundance relative to carbon building the organic matter matrix, the most likely scenario for the formation of UCAMMs occurs via physicochemical mechanisms taking place in a cold nitrogen rich environment, like the surface of icy parent bodies in the outer solar system. The composition of UCAMMs provides an additional hint of the presence of a heliocentric positive gradient in the C/Si and N/C abundance ratios in the solar system protoplanetary disc evolution. Part of the equipment used in this work has been

Malnutrition and Mortality Patterns among Internally Displaced and Non-Displaced Population Living in a Camp, a Village or a Town in Eastern Chad

PubMed Central

Guerrier, Gilles; Zounoun, Malaïka; Delarosa, Olimpia; Defourny, Isabelle; Lacharite, Michelo; Brown, Vincent; Pedalino, Biagio

2009-01-01

Background Certain population groups have been rendered vulnerable in Chad because of displacement of more than 200,000 people over the last three years as a result of mass violence against civilians in the east of the country. The objective of the study was to assess mortality and nutritional patterns among displaced and non-displaced population living in camps, villages and a town in the Ouddaï and Salamat regions of Chad. Methodology Between May and October 2007, two stage, 30-cluster household surveys were conducted among 43,900 internally displaced persons (IDPs) living in camps in Ouaddai region (n = 898 households), among 19,400 non-displaced persons (NDPs) living in 42 villages in Ouaddai region (n = 900 households) and among 17,000 NDPs living in a small town in Salamat region (n = 901 households). Data collection included anthropometric measurements, measles vaccination rates and retrospective mortality. Crude mortality rate (CMR), mortality rate among children younger than 5 years (U5MR), causes of death and the prevalence of wasting (weight-for-height z score <−2) among children aged 6 to 59 months were the main outcome measures. Conclusions The CMR among the 4902 IDPs in Gozbeida camps, 4477 NDPs living in a village and 4073 NDPs living in a town surveyed was 1.8 (95% CI, 1.2–2.8), 0.3 (95% CI, 0.2–0.4), 0.3 (95% CI, 0.2–0.5) per 10,000 per day, respectively. The U5MR in a camp (n = 904), a village (n = 956) and a town (n = 901) was 4.1 (95% CI, 2.1–7.7), 0.5 (95% CI, 0.3–0.9) and 0.7 (95% CI, 0.4–1.4) per 10,000 per day, respectively. Diarrhoea was reported to be the main cause of death. Acute malnutrition rates (according to the WHO definition) among 904 IDP children, 956 NDPs children living in a village, 901 NDP children living in a town aged 6 to 59 months were 20.6% (95% CI, 17.9%–23.3%), 16.4% (95% CI, 14.0%–18.8%) and 10.1% (95% CI, 8.1%–12.2%) respectively. The study found a high mortality rate

Malnutrition and mortality patterns among internally displaced and non-displaced population living in a camp, a village or a town in Eastern Chad.

PubMed

Guerrier, Gilles; Zounoun, Malaïka; Delarosa, Olimpia; Defourny, Isabelle; Lacharite, Michelo; Brown, Vincent; Pedalino, Biagio

2009-11-26

Certain population groups have been rendered vulnerable in Chad because of displacement of more than 200,000 people over the last three years as a result of mass violence against civilians in the east of the country. The objective of the study was to assess mortality and nutritional patterns among displaced and non-displaced population living in camps, villages and a town in the Ouddaï and Salamat regions of Chad. Between May and October 2007, two stage, 30-cluster household surveys were conducted among 43,900 internally displaced persons (IDPs) living in camps in Ouaddai region (n = 898 households), among 19,400 non-displaced persons (NDPs) living in 42 villages in Ouaddai region (n = 900 households) and among 17,000 NDPs living in a small town in Salamat region (n = 901 households). Data collection included anthropometric measurements, measles vaccination rates and retrospective mortality. Crude mortality rate (CMR), mortality rate among children younger than 5 years (U5MR), causes of death and the prevalence of wasting (weight-for-height z score <-2) among children aged 6 to 59 months were the main outcome measures. The CMR among the 4902 IDPs in Gozbeida camps, 4477 NDPs living in a village and 4073 NDPs living in a town surveyed was 1.8 (95% CI, 1.2-2.8), 0.3 (95% CI, 0.2-0.4), 0.3 (95% CI, 0.2-0.5) per 10,000 per day, respectively. The U5MR in a camp (n = 904), a village (n = 956) and a town (n = 901) was 4.1 (95% CI, 2.1-7.7), 0.5 (95% CI, 0.3-0.9) and 0.7 (95% CI, 0.4-1.4) per 10,000 per day, respectively. Diarrhoea was reported to be the main cause of death. Acute malnutrition rates (according to the WHO definition) among 904 IDP children, 956 NDPs children living in a village, 901 NDP children living in a town aged 6 to 59 months were 20.6% (95% CI, 17.9%-23.3%), 16.4% (95% CI, 14.0%-18.8%) and 10.1% (95% CI, 8.1%-12.2%) respectively. The study found a high mortality rate among IDPs and an elevated prevalence of wasting not only in IDP camps but also in

Densities of 5-15 micron interplanetary dust particles

NASA Technical Reports Server (NTRS)

Love, S. G.; Joswiak, D. J.; Brownlee, D. E.

1993-01-01

We have measured the densities of about 100 5-15 micron stratospheric IDPs. Great care was taken to minimize selection bias in the sample population. Masses were determined using an absolute x-ray analysis technique with a transmission electron microscope, and volumes were found using scanning electron microscope imagery. Unmelted chondritic particles have densities between 0.5 and 6.0 g/cc. Roughly half of the particles have densities below 2 g/cc, indicating appreciable porosity, but porosities greater than about 70 percent are rare. IDPs with densities above 3.5 g/cc usually contain large sulfide grains. We find no evidence of bimodality in the unmelted particle density distribution. Chondritic spherules (melted particles) have densities near 3.5 g/cc, consistent with previous results for deep sea spherules.

Final Assessment: U.S. Virgin Islands Industrial Development Park and Adjacent Facilities Energy-Efficiency and Micro-Grid Infrastructure

DOE Office of Scientific and Technical Information (OSTI.GOV)

Petersen, Joseph M.; Boyd, Paul A.; Dahowski, Robert T.

The purpose of this assessment was to undertake an assessment and analysis of cost-effective options for energy-efficiency improvements and the deployment of a micro-grid to increase the energy resilience at the U.S. Virgin Islands Industrial Development Park (IDP) and adjacent facilities in St. Croix, Virgin Islands. The Economic Development Authority sought assistance from the U.S. Department of Energy to undertake this assessment undertaken by Pacific Northwest National Laboratory. The assessment included 18 buildings plus the perimeter security lighting at the Virgin Islands Bureau of Correctional Facility, four buildings plus exterior lighting at the IDP, and five buildings (one of whichmore » is to be constructed) at the Virgin Islands Police Department for a total of 27 buildings with a total of nearly 323,000 square feet.« less

Skin tumor area extraction using an improved dynamic programming approach.

PubMed

Abbas, Qaisar; Celebi, M E; Fondón García, Irene

2012-05-01

Border (B) description of melanoma and other pigmented skin lesions is one of the most important tasks for the clinical diagnosis of dermoscopy images using the ABCD rule. For an accurate description of the border, there must be an effective skin tumor area extraction (STAE) method. However, this task is complicated due to uneven illumination, artifacts present in the lesions and smooth areas or fuzzy borders of the desired regions. In this paper, a novel STAE algorithm based on improved dynamic programming (IDP) is presented. The STAE technique consists of the following four steps: color space transform, pre-processing, rough tumor area detection and refinement of the segmented area. The procedure is performed in the CIE L(*) a(*) b(*) color space, which is approximately uniform and is therefore related to dermatologist's perception. After pre-processing the skin lesions to reduce artifacts, the DP algorithm is improved by introducing a local cost function, which is based on color and texture weights. The STAE method is tested on a total of 100 dermoscopic images. In order to compare the performance of STAE with other state-of-the-art algorithms, various statistical measures based on dermatologist-drawn borders are utilized as a ground truth. The proposed method outperforms the others with a sensitivity of 96.64%, a specificity of 98.14% and an error probability of 5.23%. The results demonstrate that this STAE method by IDP is an effective solution when compared with other state-of-the-art segmentation techniques. The proposed method can accurately extract tumor borders in dermoscopy images. © 2011 John Wiley & Sons A/S.

Collaboration pathway(s) using new tools for optimizing `operational' climate monitoring from space

NASA Astrophysics Data System (ADS)

Helmuth, Douglas B.; Selva, Daniel; Dwyer, Morgan M.

2015-09-01

Consistently collecting the earth's climate signatures remains a priority for world governments and international scientific organizations. Architecting a long term solution requires transforming scientific missions into an optimized robust `operational' constellation that addresses the collective needs of policy makers, scientific communities and global academic users for trusted data. The application of new tools offers pathways for global architecture collaboration. Recent rule-based expert system (RBES) optimization modeling of the intended NPOESS architecture becomes a surrogate for global operational climate monitoring architecture(s). These rulebased systems tools provide valuable insight for global climate architectures, by comparison/evaluation of alternatives and the sheer range of trade space explored. Optimization of climate monitoring architecture(s) for a partial list of ECV (essential climate variables) is explored and described in detail with dialogue on appropriate rule-based valuations. These optimization tool(s) suggest global collaboration advantages and elicit responses from the audience and climate science community. This paper will focus on recent research exploring joint requirement implications of the high profile NPOESS architecture and extends the research and tools to optimization for a climate centric case study. This reflects work from SPIE RS Conferences 2013 and 2014, abridged for simplification30, 32. First, the heavily securitized NPOESS architecture; inspired the recent research question - was Complexity (as a cost/risk factor) overlooked when considering the benefits of aggregating different missions into a single platform. Now years later a complete reversal; should agencies considering Disaggregation as the answer. We'll discuss what some academic research suggests. Second, using the GCOS requirements of earth climate observations via ECV (essential climate variables) many collected from space-based sensors; and accepting their

Dynamics, Conformational Entropy, and Frustration in Protein-Protein Interactions Involving an Intrinsically Disordered Protein Domain.

PubMed

Lindström, Ida; Dogan, Jakob

2018-05-18

Intrinsically disordered proteins (IDPs) are abundant in the eukaryotic proteome. However, little is known about the role of subnanosecond dynamics and the conformational entropy that it represents in protein-protein interactions involving IDPs. Using nuclear magnetic resonance side chain and backbone relaxation, stopped-flow kinetics, isothermal titration calorimetry, and computational studies, we have characterized the interaction between the globular TAZ1 domain of the CREB binding protein and the intrinsically disordered transactivation domain of STAT2 (TAD-STAT2). We show that the TAZ1/TAD-STAT2 complex retains considerable subnanosecond motions, with TAD-STAT2 undergoing only a partial disorder-to-order transition. We report here the first experimental determination of the conformational entropy change for both binding partners in an IDP binding interaction and find that the total change even exceeds in magnitude the binding enthalpy and is comparable to the contribution from the hydrophobic effect, demonstrating its importance in the binding energetics. Furthermore, we show that the conformational entropy change for TAZ1 is also instrumental in maintaining a biologically meaningful binding affinity. Strikingly, a spatial clustering of very high amplitude motions and a cluster of more rigid sites in the complex exist, which through computational studies we found to overlap with regions that experience energetic frustration and are less frustrated, respectively. Thus, the residual dynamics in the bound state could be necessary for faster dissociation, which is important for proteins that interact with multiple binding partners.

How Robust Is the Mechanism of Folding-Upon-Binding for an Intrinsically Disordered Protein?

PubMed

Bonetti, Daniela; Troilo, Francesca; Brunori, Maurizio; Longhi, Sonia; Gianni, Stefano

2018-04-24

The mechanism of interaction of an intrinsically disordered protein (IDP) with its physiological partner is characterized by a disorder-to-order transition in which a recognition and a binding step take place. Even if the mechanism is quite complex, IDPs tend to bind their partner in a cooperative manner such that it is generally possible to detect experimentally only the disordered unbound state and the structured complex. The interaction between the disordered C-terminal domain of the measles virus nucleoprotein (N TAIL ) and the X domain (XD) of the viral phosphoprotein allows us to detect and quantify the two distinct steps of the overall reaction. Here, we analyze the robustness of the folding of N TAIL upon binding to XD by measuring the effect on both the folding and binding steps of N TAIL when the structure of XD is modified. Because it has been shown that wild-type XD is structurally heterogeneous, populating an on-pathway intermediate under native conditions, we investigated the binding to 11 different site-directed variants of N TAIL of one particular variant of XD (I504A XD) that populates only the native state. Data reveal that the recognition and the folding steps are both affected by the structure of XD, indicating a highly malleable pathway. The experimental results are briefly discussed in the light of previous experiments on other IDPs. Copyright © 2018 Biophysical Society. Published by Elsevier Inc. All rights reserved.

High Precision Oxygen Three Isotope Analysis of Wild-2 Particles and Anhydrous Chondritic Interplanetary Dust Particles

NASA Technical Reports Server (NTRS)

Nakashima, D.; Ushikubo, T.; Zolensky, Michael E.; Weisberg, M. K.; Joswiak, D. J.; Brownlee, D. E.; Matrajt, G.; Kita, N. T.

2011-01-01

One of the most important discoveries from comet Wild-2 samples was observation of crystalline silicate particles that resemble chondrules and CAIs in carbonaceous chondrites. Previous oxygen isotope analyses of crystalline silicate terminal particles showed heterogeneous oxygen isotope ratios with delta(sup 18)O to approx. delta(sup 17)O down to -50% in the CAI-like particle Inti, a relict olivine grain in Gozen-sama, and an olivine particle. However, many Wild-2 particles as well as ferromagnesian silicates in anhydrous interplanetary dust particles (IDPs) showed Delta(sup 17)O values that cluster around -2%. In carbonaceous chondrites, chondrules seem to show two major isotope reservoirs with Delta(sup 17)O values at -5% and -2%. It was suggested that the Delta(sup 17)O = -2% is the common oxygen isotope reservoir for carbonaceous chondrite chondrules and cometary dust, from the outer asteroid belt to the Kuiper belt region. However, a larger dataset with high precision isotope analyses (+/-1-2%) is still needed to resolve the similarities or distinctions among Wild-2 particles, IDPs and chondrules in meteorites. We have made signifi-cant efforts to establish routine analyses of small particles (< or =10micronsm) at 1-2% precision using IMS-1280 at WiscSIMS laboratory. Here we report new results of high precision oxygen isotope analyses of Wild-2 particles and anhydrous chondritic IDPs, and discuss the relationship between the cometary dust and carbonaceous chondrite chondrules.

In situ 3-D mapping of pore structures and hollow grains of interplanetary dust particles with phase contrast X-ray nanotomography

NASA Astrophysics Data System (ADS)

Hu, Z. W.; Winarski, R. P.

2016-09-01

Unlocking the 3-D structure and properties of intact chondritic porous interplanetary dust particles (IDPs) in nanoscale detail is challenging, which is also complicated by atmospheric entry heating, but is important for advancing our understanding of the formation and origins of IDPs and planetary bodies as well as dust and ice agglomeration in the outer protoplanetary disk. Here, we show that indigenous pores, pristine grains, and thermal alteration products throughout intact particles can be noninvasively visualized and distinguished morphologically and microstructurally in 3-D detail down to ~10 nm by exploiting phase contrast X-ray nanotomography. We have uncovered the surprisingly intricate, submicron, and nanoscale pore structures of a ~10-μm-long porous IDP, consisting of two types of voids that are interconnected in 3-D space. One is morphologically primitive and mostly submicron-sized intergranular voids that are ubiquitous; the other is morphologically advanced and well-defined intragranular nanoholes that run through the approximate centers of ~0.3 μm or lower submicron hollow grains. The distinct hollow grains exhibit complex 3-D morphologies but in 2-D projections resemble typical organic hollow globules observed by transmission electron microscopy. The particle, with its outer region characterized by rough vesicular structures due to thermal alteration, has turned out to be an inherently fragile and intricately submicron- and nanoporous aggregate of the sub-μm grains or grain clumps that are delicately bound together frequently with little grain-to-grain contact in 3-D space.

Papillary lesions of the breast: To excise or observe?

PubMed

Khan, Sidrah; Diaz, Adrian; Archer, Kellie J; Lehman, Rebecca R; Mullins, Tiffany; Cardenosa, Gilda; Bear, Harry D

2018-05-01

Papillary lesions of the breast range from benign to atypical to malignant. Although papillomas without frank cancer are benign, their management remains controversial. When a core needle biopsy of a lesion yields a diagnosis of intraductal papilloma with atypia, excision is generally recommended to rule out a concurrent malignant neoplasm. For intraductal papillomas without atypia, however, recommendations for excision versus observation are variable. The aims of this study are to evaluate the rate of concurrent malignancies for intraductal papilloma diagnosed on core needle biopsy and to assess the long-term risk of developing cancer after the diagnosis of a papillary lesion. This single institution retrospective study analyzed 259 patients that were diagnosed with intraductal papilloma (IDP) by core needle biopsy from 1995 to 2010. Patients were grouped by initial diagnosis into three groups (papilloma without atypia, papilloma with atypia, and papilloma with atypical duct hyperplasia or atypical lobular hyperplasia (ADH/ALH) and followed up for long-term outcomes. After a core needle biopsy showing IDP with atypia or IDP + ADH/ALH, surgical excision yielded a diagnosis of concomitant invasive or ductal in situ cancer in greater that 30% of cases. For intraductal papilloma without atypia, the likelihood of cancer was much lower. Moreover, even with excision, the finding of intraductal papilloma with atypia carries a significant risk of developing cancer long-term, and such patients should be followed carefully and perhaps should be considered for chemoprevention. © 2017 Wiley Periodicals, Inc.

Student performance in a flipped classroom dental anatomy course.

PubMed

Chutinan, S; Riedy, C A; Park, S E

2017-11-09

The purpose of this study was to assess dental student learning in a dental anatomy module between traditional lecture and flipped classroom cohorts. Two cohorts of predoctoral dental students (N = 70 within each cohort) participated in a dental anatomy module within an Introduction to the Dental Patient (IDP) course ([traditional/lecture cohort: academic year (AY) 2012, 2013] and [flipped classroom cohort: AY 2014, 2015]). For the dental anatomy module, both cohorts were evaluated on pre-clinical tooth waxing exercises immediately after each of five lectures and tooth identification after all lectures were given. Additionally, the cohorts' performance on the overall IDP course examination was compared. The flipped classroom cohort had statistically significant higher waxing scores (dental anatomy module) than students in the traditional classroom. There was no statistically significant difference for tooth identification scores and the overall IDP course examination between the traditional vs flipped approach cohorts. This is due to the latter two assessments conducted at the end of the course gave all students enough time to review the lecture content prior to the assessment resulting in similar scores for both cohorts. The flipped classroom cohort promoted students' individual learning and resulted in improved students' performance on immediate evaluation but not on the end of the course evaluation. Redesign of courses to include a new pedagogical approach should be carefully implemented and evaluated for student's educational success. © 2017 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.

Trapped Xenon in Interplanetary Dust Particles and Antarctic Micrometeorites

NASA Astrophysics Data System (ADS)

Spring, N. H.; Busemann, H.; Crowther, S. A.; Gilmour, J. D.

2014-09-01

We analysed the Xe inventory of several IDPs and AMMs and infer that cometary solids may contain the same mix of organic matter and trapped Xe carriers as found in carbonaceous chondrite matrices, although in higher relative abundances.

The REFANI-S study protocol: a non-randomised cluster controlled trial to assess the role of an unconditional cash transfer, a non-food item kit, and free piped water in reducing the risk of acute malnutrition among children aged 6-59 months living in camps for internally displaced persons in the Afgooye corridor, Somalia.

PubMed

Jelle, Mohamed; Grijalva-Eternod, Carlos S; Haghparast-Bidgoli, Hassan; King, Sarah; Cox, Cassy L; Skordis-Worrall, Jolene; Morrison, Joanna; Colbourn, Timothy; Fottrell, Edward; Seal, Andrew J

2017-07-06

The prevalence of acute malnutrition is often high in emergency-affected populations and is associated with elevated mortality risk and long-term health consequences. Increasingly, cash transfer programmes (CTP) are used instead of direct food aid as a nutritional intervention, but there is sparse evidence on their nutritional impact. We aim to understand whether CTP reduces acute malnutrition and its known risk factors. A non-randomised, cluster-controlled trial will assess the impact of an unconditional cash transfer of US$84 per month for 5 months, a single non-food items kit, and free piped water on the risk of acute malnutrition in children, aged 6-59 months. The study will take place in camps for internally displaced persons (IDP) in peri-urban Mogadishu, Somalia. A cluster will consist of one IDP camp and 10 camps will be allocated to receive the intervention based on vulnerability targeting criteria. The control camps will then be selected from the same geographical area. Needs assessment data indicates small differences in vulnerability between camps. In each trial arm, 120 households will be randomly sampled and two detailed household surveys will be implemented at baseline and 3 months after the initiation of the cash transfer. The survey questionnaire will cover risk factors for malnutrition including household expenditure, assets, food security, diet diversity, coping strategies, morbidity, WASH, and access to health care. A community surveillance system will collect monthly mid-upper arm circumference measurements from all children aged 6-59 months in the study clusters to assess the incidence of acute malnutrition over the duration of the intervention. Process evaluation data will be compiled from routine quantitative programme data and primary qualitative data collected using key informant interviews and focus group discussions. The UK Department for International Development will provide funding for this study. The European Civil Protection and

Collected Extraterrestrial Materials: Constraints on Meteor and Fireball Compositions

NASA Astrophysics Data System (ADS)

Rietmeijer, Frans J. M.; Nuth, Joseph A., III

The bulk density and bulk porosity of IDPs and various meteorite classes show that protoplanet accretion and evolution were arrested at different stages as a function of parent body modification. The collected IDPs, micrometeorites and meteorites are aggregates of different structural entities that were inherited from the earliest times of solar system evolution. These structural entities and the extent of parent body lithification will determine the material strength of the meteoroids entering the Earth's atmosphere. There is a need for measurements of the material strength of collected extraterrestrial materials because they will in part determine the nature of the chemical interactions of descending meteors and fireballs in the atmosphere. High-precision determinations of meteor and fireball compositions are required to search for anhydrous, carbon-rich proto-CI material that has survived in the boulders of comet nuclei.

SeaWiFS long-term solar diffuser reflectance and sensor noise analyses

NASA Astrophysics Data System (ADS)

Eplee, Robert E., Jr.; Patt, Frederick S.; Barnes, Robert A.; McClain, Charles R.

2007-02-01

The NASA Ocean Biology Processing Group's Calibration and Validation (Cal/Val) team has undertaken an analysis of the mission-long Sea-Viewing Wide Field-of-View Sensor (SeaWiFS) solar calibration time series to assess the long-term degradation of the solar diffuser reflectance over 9 years on orbit. The SeaWiFS diffuser is an aluminum plate coated with YB71 paint. The bidirectional reflectance distribution function of the diffuser was not fully characterized before launch, so the Cal/Val team has implemented a regression of the solar incidence angles and the drift in the node of the satellite's orbit against the diffuser time series to correct for solar incidence angle effects. An exponential function with a time constant of 200 days yields the best fit to the diffuser time series. The decrease in diffuser reflectance over the mission is wavelength dependent, ranging from 9% in the blue (412 nm) to 5% in the red and near infrared (670-865 nm). The Cal/Val team has developed a methodology for computing the signal-to-noise ratio (SNR) for SeaWiFS on orbit from the diffuser time series corrected for both the varying solar incidence angles and the diffuser reflectance degradation. A sensor noise model is used to compare on-orbit SNRs computed for radiances reflected from the diffuser with prelaunch SNRs measured at typical radiances specified for the instrument. To within the uncertainties in the measurements, the SNRs for SeaWiFS have not changed over the mission. The on-orbit performance of the SeaWiFS solar diffuser should offer insight into the long-term on-orbit performance of solar diffusers on other instruments, such as the Moderate-Resolution Imaging Spectrometer [currently flying on the Earth Observing System (EOS) Terra and Aqua satellites], the Visible and Infrared Radiometer Suite [scheduled to fly on the NASA National Polar-orbiting Operational Environmental Satellite System (NPOESS) and NPOESS Preparatory Project (NPP) satellites] and the Advanced

SeaWiFS long-term solar diffuser reflectance and sensor noise analyses.

PubMed

Eplee, Robert E; Patt, Frederick S; Barnes, Robert A; McClain, Charles R

2007-02-10

The NASA Ocean Biology Processing Group's Calibration and Validation (Cal/Val) team has undertaken an analysis of the mission-long Sea-Viewing Wide Field-of-View Sensor (SeaWiFS) solar calibration time series to assess the long-term degradation of the solar diffuser reflectance over 9 years on orbit. The SeaWiFS diffuser is an aluminum plate coated with YB71 paint. The bidirectional reflectance distribution function of the diffuser was not fully characterized before launch, so the Cal/Val team has implemented a regression of the solar incidence angles and the drift in the node of the satellite's orbit against the diffuser time series to correct for solar incidence angle effects. An exponential function with a time constant of 200 days yields the best fit to the diffuser time series. The decrease in diffuser reflectance over the mission is wavelength dependent, ranging from 9% in the blue (412 nm) to 5% in the red and near infrared (670-865 nm). The Cal/Val team has developed a methodology for computing the signal-to-noise ratio (SNR) for SeaWiFS on orbit from the diffuser time series corrected for both the varying solar incidence angles and the diffuser reflectance degradation. A sensor noise model is used to compare on-orbit SNRs computed for radiances reflected from the diffuser with prelaunch SNRs measured at typical radiances specified for the instrument. To within the uncertainties in the measurements, the SNRs for SeaWiFS have not changed over the mission. The on-orbit performance of the SeaWiFS solar diffuser should offer insight into the long-term on-orbit performance of solar diffusers on other instruments, such as the Moderate-Resolution Imaging Spectrometer [currently flying on the Earth Observing System (EOS) Terra and Aqua satellites], the Visible and Infrared Radiometer Suite [scheduled to fly on the NASA National Polar-orbiting Operational Environmental Satellite System (NPOESS) and NPOESS Preparatory Project (NPP) satellites] and the Advanced

SeaWiFS long-term solar diffuser reflectance and sensor noise analyses

DOE Office of Scientific and Technical Information (OSTI.GOV)

Eplee, Robert E. Jr.; Patt, Frederick S.; Barnes, Robert A.

The NASA Ocean Biology Processing Group's Calibration and Validation(Cal/Val) team has undertaken an analysis of the mission-long Sea-Viewing Wide Field-of-View Sensor (SeaWiFS)solar calibration time series to assess the long-term degradation of the solar diffuser reflectance over 9 years on orbit. The SeaWiFS diffuser is an aluminum plate coated with YB71 paint. The bidirectional reflectance distribution function of the diffuser was not fully characterized before launch,so the Cal/Val team has implemented a regression of the solar incidence angles and the drift in the node of the satellite's orbit against the diffuser time series to correct for solar incidence angle effects. Anmore » exponential function with a time constant of 200 days yields the best fit to the diffuser time series.The decrease in diffuser reflectance over the mission is wavelength dependent,ranging from 9% in the blue(412 nm) to 5% in the red and near infrared(670-865 nm). The Cal/Val team has developed a methodology for computing the signal-to-noise ratio (SNR) for SeaWiFS on orbit from the diffuser time series corrected for both the varying solar incidence angles and the diffuser reflectance degradation. A sensor noise model is used to compare on-orbit SNRs computed for radiances reflected from the diffuser with prelaunch SNRs measured at typical radiances specified for the instrument. To within the uncertainties in the measurements, the SNRs for SeaWiFS have not changed over the mission. The on-orbit performance of the SeaWiFS solar diffuser should offer insight into the long-term on-orbit performance of solar diffusers on other instruments, such as the Moderate-Resolution Imaging Spectrometer [currently flying on the Earth Observing System (EOS) Terra and Aqua satellites], the Visible and Infrared Radiometer Suite [scheduled to fly on the NASA National Polar-orbiting Operational Environmental Satellite System (NPOESS) and NPOESS Preparatory Project (NPP) satellites] and the Advanced

An Overview of Future NASA Missions, Concepts, and Technologies Related to Imaging of the World's Land Areas

NASA Technical Reports Server (NTRS)

Salomonson, Vincent V.

1999-01-01

In the near term NASA is entering into the peak activity period of the Earth Observing System (EOS). The EOS AM-1 /"Terra" spacecraft is nearing launch and operation to be followed soon by the New Millennium Program (NMP) Earth Observing (EO-1) mission. Other missions related to land imaging and studies include EOS PM-1 mission, the Earth System Sciences Program (ESSP) Vegetation Canopy Lidar (VCL) mission, the EOS/IceSat mission. These missions involve clear advances in technologies and observational capability including improvements in multispectral imaging and other observing strategies, for example, "formation flying". Plans are underway to define the next era of EOS missions, commonly called "EOS Follow-on" or EOS II. The programmatic planning includes concepts that represent advances over the present Landsat-7 mission that concomitantly recognize the advances being made in land imaging within the private sector. The National Polar Orbiting Environmental Satellite Series (NPOESS) Preparatory Project (NPP) is an effort that will help to transition EOS medium resolution (herein meaning spatial resolutions near 500 meters), multispectral measurement capabilities such as represented by the EOS Moderate Resolution Imaging Spectroradiometer (MODIS) into the NPOESS operational series of satellites. Developments in Synthetic Aperture Radar (SAR) and passive microwave land observing capabilities are also proceeding. Beyond these efforts the Earth Science Enterprise Technology Strategy is embarking efforts to advance technologies in several basic areas: instruments, flight systems and operational capability, and information systems. In the case of instruments architectures will be examined that offer significant reductions in mass, volume, power and observational flexibility. For flight systems and operational capability, formation flying including calibration and data fusion, systems operation autonomy, and mechanical and electronic innovations that can reduce

Physics of interplanetary dust capture via impact into organic polymer foams

NASA Technical Reports Server (NTRS)

Anderson, William W.; Ahrens, Thomas J.

1994-01-01

The physics of hypervelocity impacts into foams is of interest because of the possible application to interplanetary dust particle (IDP) capture by spacecraft. We present a model for the phenomena occurring in such impacts into low-density organic polymer foams. Particles smaller than foam cells behave as if the foam is a series of solid slabs and are fragmented and, at higher velocities, thermally altered. Particles much larger than the foam cells behave as if the foam were a continuum, allowing the use of a continuum mechanics model to describe the effects of drag and ablation. Fragmentation is expected to be a major process, especially for aggregates of small grains. Calculations based on these arguments accurately predict experimental data and, for hypothetical IDPs, indicate that recovery of organic materials will be low for encounter velocities greater than 5 km/s. For an organic particle 100 micrometers in diameter, approx. 35% of the original mass would be collected in an impact at 5 km/s, dropping to approx. 10% at 10 km/s and approx. 0% at 15 km/s. For the same velocities the recovery ratios for troilite (FeS) are approx. 95%, 65%, and 50%, and for olivine (Mg2SiO4) they are approx. 98%, 80%, and 65%, demonstrating that inorganic materials are much more easily collected. The density of the collector material has only a second-order effect, changing the recovered mass by less than 10% of the original mass.

Cometary Evolution: Clues on Physical Properties from Chondritic Interplanetary Dust Particles

NASA Technical Reports Server (NTRS)

Reitmeijer, Frans J. M.; Mackinnon, Ian D. R.

1997-01-01

chondritic interplanetary dust particles (IDPS) as a guide to the likely constitution of mature comets traversing the inner Solar System. While there is, as yet, no direct proof that a specific sub-group or type of chondritic IDP is derived from a specific comet, it is clear that these particles are extraterrestrial in origin and that a certain portion of the interplanetary flux received by the Earth is cometary in origin. Two chondritic porous (CP) IDPS, sample numbers W701OA2 and W7029CI, from the Johnson Space Center Cosmic Dust Collection have been selected for this study of putative cometary physical parameters. This particular type of particle is considered a likely candidate for a cometary origin on the basis of mineralogy, bulk composition and morphology. While many IDPs have been subjected to intensive study over the past decade, we can develop a physical parameter model on only these two CP IDPs because few others have been studied in sufficient detail.

Progress towards MODIS and VIIRS Cloud Fraction Data Record Continuity

NASA Astrophysics Data System (ADS)

Ackerman, S. A.; Frey, R.; Holz, R.; Platnick, S. E.; Heidinger, A. K.

2016-12-01

Satellite-derived clear-sky vs. cloudy-sky discrimination at the pixel scale is an important input parameter used in many real-time applications. Cloud fractions, resulting from integrating over time and space, are also critical to the study of recent decadal climate changes. The NASA NPOESS Preparatory Project (NPP) has funded a science team to develop and study the ability to make continuous climate records from MODIS (2000-2020) and VIIRS (2012-2030). The MODAWG project, led by Dr. Steve Platnick of NASA/GSFC, combines elements of the MODIS processing system and the NOAA Algorithm Working Group (AWG) to achieve this goal. This presentation will focus on the cloud masking aspects of MODAWG, derived primarily from the MODIS cloud mask (MOD35). Challenges to continuity of cloud detection due to differences in instrument characteristics will be discussed. Cloud mask results from use of the same (continuity) algorithm will be shown for both MODIS and VIIRS, including comparisons to collocated CALIOP (Cloud-Aerosol Lidar with Orthogonal Polarization) cloud data.

Gender-based violence in conflict and displacement: qualitative findings from displaced women in Colombia.

PubMed

Wirtz, Andrea L; Pham, Kiemanh; Glass, Nancy; Loochkartt, Saskia; Kidane, Teemar; Cuspoca, Decssy; Rubenstein, Leonard S; Singh, Sonal; Vu, Alexander

2014-01-01

Gender-based violence (GBV) is prevalent among, though not specific to, conflict affected populations and related to multifarious levels of vulnerability of conflict and displacement. Colombia has been marked with decades of conflict, with an estimated 5.2 million internally displaced persons (IDPs) and ongoing violence. We conducted qualitative research to understand the contexts of conflict, displacement and dynamics with GBV. This as part of a multi-phase, mixed method study, in collaboration with UNHCR, to develop a screening tool to confidentially identify cases of GBV for referral among IDP women who were survivors of GBV. Qualitative research was used to identify the range of GBV, perpetrators, contexts in conflict and displacement, barriers to reporting and service uptake, as well as to understand experiences of service providers. Thirty-five female IDPs, aged 18 years and older, who self-identified as survivors of GBV were enrolled for in-depth interviews in San Jose de Guaviare and Quibdo, Colombia in June 2012. Thirty-one service providers participated in six focus group discussions and four interviews across these sites. Survivors described a range of GBV across conflict and displacement settings. Armed actors in conflict settings perpetrated threats of violence and harm to family members, child recruitment, and, to a lesser degree, rape and forced abortion. Opportunistic violence, including abduction, rape, and few accounts of trafficking were more commonly reported to occur in the displacement setting, often perpetrated by unknown individuals. Intrafamilial violence, intimate partner violence, including physical and sexual violence and reproductive control were salient across settings and may be exacerbated by conflict and displacement. Barriers to reporting and services seeking were reported by survivors and providers alike. Findings highlight the need for early identification of GBV cases, with emphasis on confidential approaches and active

Proton-decoupled CPMG: a better experiment for measuring (15)N R2 relaxation in disordered proteins.

PubMed

Yuwen, Tairan; Skrynnikov, Nikolai R

2014-04-01

(15)N R2 relaxation is one of the most informative experiments for characterization of intrinsically disordered proteins (IDPs). Small changes in nitrogen R2 rates are often used to determine how IDPs respond to various biologically relevant perturbations such as point mutations, posttranslational modifications and weak ligand interactions. However collecting high-quality (15)N relaxation data can be difficult. Of necessity, the samples of IDPs are often prepared with low protein concentration and the measurement time can be limited because of rapid sample degradation. Furthermore, due to hardware limitations standard experiments such as (15)N spin-lock and CPMG can sample the relaxation decay only to ca. 150ms. This is much shorter than (15)N T2 times in disordered proteins at or near physiological temperature. As a result, the sampling of relaxation decay profiles in these experiments is suboptimal, which further lowers the precision of the measurements. Here we report a new implementation of the proton-decoupled (PD) CPMG experiment which allows one to sample (15)N R2 relaxation decay up to ca. 0.5-1s. The new experiment has been validated through comparison with the well-established spin-lock measurement. Using dilute samples of denatured ubiquitin, we have demonstrated that PD-CPMG produces up to 3-fold improvement in the precision of the data. It is expected that for intrinsically disordered proteins the gains may be even more substantial. We have also shown that this sequence has a number of favorable properties: (i) the spectra are recorded with narrow linewidth in nitrogen dimension; (ii) (15)N offset correction is small and easy to calculate; (iii) the experiment is immune to various spurious effects arising from solvent exchange; (iv) the results are stable with respect to pulse miscalibration and rf field inhomogeneity; (v) with minimal change, the pulse sequence can also be used to measure R2 relaxation of (15)N(ε) spins in arginine side chains. We

Gender-based violence in conflict and displacement: qualitative findings from displaced women in Colombia

PubMed Central

2014-01-01

Introduction Gender-based violence (GBV) is prevalent among, though not specific to, conflict affected populations and related to multifarious levels of vulnerability of conflict and displacement. Colombia has been marked with decades of conflict, with an estimated 5.2 million internally displaced persons (IDPs) and ongoing violence. We conducted qualitative research to understand the contexts of conflict, displacement and dynamics with GBV. This as part of a multi-phase, mixed method study, in collaboration with UNHCR, to develop a screening tool to confidentially identify cases of GBV for referral among IDP women who were survivors of GBV. Methods Qualitative research was used to identify the range of GBV, perpetrators, contexts in conflict and displacement, barriers to reporting and service uptake, as well as to understand experiences of service providers. Thirty-five female IDPs, aged 18 years and older, who self-identified as survivors of GBV were enrolled for in-depth interviews in San Jose de Guaviare and Quibdo, Colombia in June 2012. Thirty-one service providers participated in six focus group discussions and four interviews across these sites. Results Survivors described a range of GBV across conflict and displacement settings. Armed actors in conflict settings perpetrated threats of violence and harm to family members, child recruitment, and, to a lesser degree, rape and forced abortion. Opportunistic violence, including abduction, rape, and few accounts of trafficking were more commonly reported to occur in the displacement setting, often perpetrated by unknown individuals. Intrafamilial violence, intimate partner violence, including physical and sexual violence and reproductive control were salient across settings and may be exacerbated by conflict and displacement. Barriers to reporting and services seeking were reported by survivors and providers alike. Conclusions Findings highlight the need for early identification of GBV cases, with emphasis on

Further analysis of LDEF FRECOPA micrometeroid remnants

NASA Technical Reports Server (NTRS)

Borg, J.; Bunch, T. E.; Radicatidibrozolo, Filippo

1992-01-01

Experiments dedicated to the detection of interplanetary dust particles (IDP's) were exposed within the FRECOPA payload, installed on the face of the LDEF directly opposed to the velocity vector (west facing direction, location B3). We were mainly interested in the analysis of hypervelocity impact features of sizes less than or = 10 microns, found in thick Al targets devoted to the research of impact features. In the 15 craters found in the scanned area (approximately 4 sq. cm), the chemical analysis suggests an extraterrestrial origin for the impacting particles. The main elements we identified are usually refered to as chondrite elements: Na, Mg, Si, S, Ca, and Fe are found in various proportions, intrinsic Al being masked by the Al target; we notice a strong depletion in Ni, never observed in our samples. Furthermore, C and O are present in 90 percent of the cases; the C/O peak height ratio varies from 0.1 to 3. Impactor simulations by light gas gun hypervelocity impact experiments have shown that meaningful biogenic element and compound information maybe obtained from IDP residues below impacts of critical velocities, that are less than or = 4 km/sec for particles larger than 100 microns in diameter. Our results obtained for the smaller size fraction IDP's suggest that at such sizes, the critical velocity could be higher by a factor of 2 or 3, as chemical analysis of the remnants were possible in all the identified impact craters, performed on targets possibly hit at velocities greater than or = 7.5 km/s, which is the spacecraft velocity. These samples are now subjected to an imagery and analytical protocol that includes FESEM (field emission scanning electron microscopy) and LIMS (laser ionization mass spectrometry). The LIMS analyses were performed using the LIMA-ZA instrument. Results are presented, clearly indicating that such small events show crater features analogous to what is observed at larger sizes; our first analytical results, obtained for 2 events

OTOTOXICITY OF 3,3'-IMINODIPROPIONITRILE: FUNCTIONAL AND MORPHOLOGICAL EVIDENCE OF COCHLEAR DAMAGE

EPA Science Inventory

Previous reports suggested that IDPN may be ototoxic (Wolff et al. 1977; Crofton and Knight, 1991). The purpose of this research was to investigate the ototoxicity of IDPN using behavioral, physiological and morphological approaches. Three groups of adult rats were exposed to IDP...

Thermal performance of the CrIS passive cryocooler

NASA Astrophysics Data System (ADS)

Ghaffarian, B.; Kohrman, R.; Magner, A.

2006-02-01

The configuration, performance, and test validation of a passive radiant cooler for the National Polar-orbiting Operational Environmental Satellite System (NPOESS) Crosstrack Infrared Sounder (CrIS) Instrument are presented. The cooler is required to provide cryogenic operation of IR focal planes. The 11 kg device, based on prior ITT Industries Space Systems Division coolers, requires virtually no power. It uses multiple thermally isolated cooling stages, each with an independent cryoradiator, operating at successively colder temperatures. The coldest stage, with a controlled set point at 81 K, cools a longwave IR (LWIR) focal plane. An intermediate stage, with a 98 K control point, cools detectors operating in MWIR and SWIR spectral regions. The warmest stage includes a fixed, integral earth shield that limits the thermal load from the earth in the NPOESS Operational Low-earth Orbiting (LEO) orbit. A study of the thermal balance and loads analysis used to evaluate the predicted cooler performance is discussed. High performance margins have been retained throughout the cooler development, fabrication and test phases of the program. The achievable in-orbit temperatures for this cooler are anticipated to be 73 K for the LWIR cooling stage and 91 K for the midwave IR (MWIR)/shortwave IR (SWIR) stage. Test results from two iterations of thermal vacuum verification testing are presented. Lessons learned from the first test, which failed to produce the predicted performance are included. The thermal model of the cooler and test configuration was used to identify deficiencies in the test targets resulting in unexpected heat loads. Corrective action was implemented to remove the heat leaks and a second test verified both the cooler performance and the correlation of the detailed thermal model.

3D Observation of GEMS by Electron Tomography

NASA Technical Reports Server (NTRS)

Matsuno, Junya; Miyake, Akira; Tsuchiyama, Akira; Nakamura-Messenger, Keiko; Messenger, Scott

2014-01-01

Amorphous silicates in chondritic porous interplanetary dust particles (CP-IDPs) coming from comets are dominated by glass with embedded metal and sulfides (GEMS). GEMS grains are submicron-sized rounded objects (typically 100-500) nm in diameter) with anaometer-sized (10-50 nm) Fe-Ni metal and sulfide grains embedded in an amorphous silicate matrix. Several formation processes for GEMS grains have been proposed so far, but these models are still being debated [2-5]. Bradley et al. proposed that GEMS grains are interstellar silicate dust that survived various metamorphism or alteration processes in the protoplanetary disk and that they are amorphiation products of crystalline silicates in the interstellar medium by sputter-deposition of cosmic ray irradiation, similar to space weathering [2,4]. This consideration is based on the observation of nano-sized crystals (approximately 10 nm) called relict grains in GEMS grains and their shapes are pseudomorphs to the host GEMS grains. On the other hand, Keller and Messenger proposed that most GEMS formed in the protoplanetary disk as condensates from high temperature gas [3,5]. This model is based on the fact that most GEMS grains have solar isotopic compositions and have extremely heterogeneous and non-solar elemental compositions. Keller and Messenger (2011) also reported that amorphous silicates in GEMS grains are surrounded by sulfide grains, which formed as sulfidization of metallic iron grains located on the GEMS surface. The previous studies were performed with 2D observation by using transmission electron microscopy (TEM) or scanning TEM (STEM). In order to understand the structure of GEMS grains described above more clearly, we observed 3D structure of GEMS grains by electron tomography using a TEM/STEM (JEM-2100F, JEOL) at Kyoto University. Electron tomography gives not only 3D structures but also gives higher spatial resolution (approximately a few nm) than that in conventional 2D image, which is restricted by

Abiogenic synthesis of nucleotides on the surface of small space bodies with high energy particles

NASA Astrophysics Data System (ADS)

Simakov, M. B.; Kuzicheva, E. A.; Antropov, A. E.; Dodonova, N. Ya

Abiotic formation of such complex biochemical compounds as nucleotides and oligopeptides on the surface of interstellar and interplanetary dust particles (IDP) by cosmic radiation was examined. In order to study the formation of organic compounds on IDPs, solid films prepared from nucleososide and inorganic phosphate were irradiated with high energy protons. Irradiated products were analyzed with HPLC. The natural nucleotides were detected. The main products were 5' AMP (3.2%) and 2'3' cAMP (2.7%). The results were compared with others experiments on the action of ultraviolet radiation with different wavelengths, γ-radiation and heat on solid mixtures of biologically significant compounds. The experiment on abiogenic synthesis of nucleotides on board of space satellite "BION-11" was compared also. The present results suggest that a considerable amount of complex biochemical compounds formed in extraterrestrial environments could have been supplied to the primitive earth before the origin of life.

Pristine extraterrestrial material with unprecedented nitrogen isotopic variation.

PubMed

Briani, Giacomo; Gounelle, Matthieu; Marrocchi, Yves; Mostefaoui, Smail; Leroux, Hugues; Quirico, Eric; Meibom, Anders

2009-06-30

Pristine meteoritic materials carry light element isotopic fractionations that constrain physiochemical conditions during solar system formation. Here we report the discovery of a unique xenolith in the metal-rich chondrite Isheyevo. Its fine-grained, highly pristine mineralogy has similarity with interplanetary dust particles (IDPs), but the volume of the xenolith is more than 30,000 times that of a typical IDP. Furthermore, an extreme continuum of N isotopic variation is present in this xenolith: from very light N isotopic composition (delta(15)N(AIR) = -310 +/- 20 per thousand), similar to that inferred for the solar nebula, to the heaviest ratios measured in any solar system material (delta(15)N(AIR) = 4,900 +/- 300 per thousand). At the same time, its hydrogen and carbon isotopic compositions exhibit very little variation. This object poses serious challenges for existing models for the origin of light element isotopic anomalies.

Complete Tem-Tomography: 3D Structure of Gems Cluster

NASA Technical Reports Server (NTRS)

Matsuno, J.; Miyake, A.; Tsuchiyama, A.; Messenger, S.; Nakamura-Messenger, K.

2015-01-01

GEMS (glass with embedded metal and sulfide) grains in interplanetary dust particles (IDPs) are considered to be one of the ubiquitous and fundamental building blocks of solids in the Solar System. They have been considered to be interstellar silicate dust that survived various metamorphism or alteration processes in the protoplanetary disk but the elemental and isotopic composition measurements suggest that most of them have been formed in the protoplanetary disk as condensates from high temperature gas. This formation model is also supported by the formation of GEMS-like grains with respect to the size, mineral assemblage, texture and infrared spectrum by condensation experiments from mean GEMS composition materials. Previous GEMS studies were performed only with 2D observation by transmission electron microscopy (TEM) or scanning TEM (STEM). However, the 3D shape and structure of GEMS grains and the spatial distribution of Fe/FeS's has critical information about their formation and origin. Recently, the 3D structure of GEMS grains in ultrathin sections of cluster IDPs was revealed by electron tomography using a TEM/STEM (JEM-2100F, JEOL). However, CT images of thin sections mounted on Cu grids acquired by conventional TEM-tomography are limited to low tilt angles (e. g., less than absolute value of 75 deg. In fact, previous 3D TEM observations of GEMS were affected by some artifacts related to the limited tilt range in the TEM used. Complete tomographic images should be acquired by rotating the sample tilt angle over a range of more than absolute value of 80 deg otherwise the CT images lose their correct structures. In order to constrain the origin and formation process of GEMS grains more clearly, we performed complete electron tomography for GEMS grains. Here we report the sample preparation method we have developed for this study, and the preliminary results.

Decoupling of size and shape fluctuations in heteropolymeric sequences reconciles discrepancies in SAXS vs. FRET measurements

PubMed Central

Fuertes, Gustavo; Banterle, Niccolò; Ruff, Kiersten M.; Chowdhury, Aritra; Mercadante, Davide; Koehler, Christine; Kachala, Michael; Estrada Girona, Gemma; Milles, Sigrid; Mishra, Ankur; Onck, Patrick R.; Gräter, Frauke; Esteban-Martín, Santiago; Pappu, Rohit V.; Svergun, Dmitri I.; Lemke, Edward A.

2017-01-01

Unfolded states of proteins and native states of intrinsically disordered proteins (IDPs) populate heterogeneous conformational ensembles in solution. The average sizes of these heterogeneous systems, quantified by the radius of gyration (RG), can be measured by small-angle X-ray scattering (SAXS). Another parameter, the mean dye-to-dye distance (RE) for proteins with fluorescently labeled termini, can be estimated using single-molecule Förster resonance energy transfer (smFRET). A number of studies have reported inconsistencies in inferences drawn from the two sets of measurements for the dimensions of unfolded proteins and IDPs in the absence of chemical denaturants. These differences are typically attributed to the influence of fluorescent labels used in smFRET and to the impact of high concentrations and averaging features of SAXS. By measuring the dimensions of a collection of labeled and unlabeled polypeptides using smFRET and SAXS, we directly assessed the contributions of dyes to the experimental values RG and RE. For chemically denatured proteins we obtain mutual consistency in our inferences based on RG and RE, whereas for IDPs under native conditions, we find substantial deviations. Using computations, we show that discrepant inferences are neither due to methodological shortcomings of specific measurements nor due to artifacts of dyes. Instead, our analysis suggests that chemical heterogeneity in heteropolymeric systems leads to a decoupling between RE and RG that is amplified in the absence of denaturants. Therefore, joint assessments of RG and RE combined with measurements of polymer shapes should provide a consistent and complete picture of the underlying ensembles. PMID:28716919

Understanding the Structural Ensembles of a Highly Extended Disordered Protein†

PubMed Central

Daughdrill, Gary W.; Kashtanov, Stepan; Stancik, Amber; Hill, Shannon E.; Helms, Gregory; Muschol, Martin

2013-01-01

Developing a comprehensive description of the equilibrium structural ensembles for intrinsically disordered proteins (IDPs) is essential to understanding their function. The p53 transactivation domain (p53TAD) is an IDP that interacts with multiple protein partners and contains numerous phosphorylation sites. Multiple techniques were used to investigate the equilibrium structural ensemble of p53TAD in its native and chemically unfolded states. The results from these experiments show that the native state of p53TAD has dimensions similar to a classical random coil while the chemically unfolded state is more extended. To investigate the molecular properties responsible for this behavior, a novel algorithm that generates diverse and unbiased structural ensembles of IDPs was developed. This algorithm was used to generate a large pool of plausible p53TAD structures that were reweighted to identify a subset of structures with the best fit to small angle X-ray scattering data. High weight structures in the native state ensemble show features that are localized to protein binding sites and regions with high proline content. The features localized to the protein binding sites are mostly eliminated in the chemically unfolded ensemble; while, the regions with high proline content remain relatively unaffected. Data from NMR experiments support these results, showing that residues from the protein binding sites experience larger environmental changes upon unfolding by urea than regions with high proline content. This behavior is consistent with the urea-induced exposure of nonpolar and aromatic side-chains in the protein binding sites that are partially excluded from solvent in the native state ensemble. PMID:21979461

What Factors Contribute to Intimate Partner Violence Against Women in Urban, Conflict-Affected Settings? Qualitative Findings from Abidjan, Côte d'Ivoire.

PubMed

Cardoso, L F; Gupta, J; Shuman, S; Cole, H; Kpebo, D; Falb, K L

2016-04-01

Rapid urbanization is a key driver of the unique set of health risks facing urban populations. One of the most critical health hazards facing urban women is intimate partner violence (IPV). In post-conflict urban areas, women may face an even greater risk of IPV. Yet, few studies have examined the IPV experiences of urban-dwelling, conflict-affected women, including those who have been internally displaced. This study qualitatively examined the social and structural characteristics of the urban environment that contributed to the IPV experiences of women residing in post-conflict Abidjan, Côte d'Ivoire. Ten focus groups were conducted with men and women, both internally displaced (IDPs) and non-displaced. Lack of support networks, changing gender roles, and tensions between traditional gender norms and those of the "modern" city were reported as key contributors to IPV. Urban poverty and with it unemployment, food insecurity, and housing instability also played a role. Finally, IDPs faced heightened vulnerability to IPV as a result of displacement and discrimination. The relationship between economic strains and IPV are similar to other conflict-affected settings, but Abidjan's urban environment presented other unique characteristics contributing to IPV. Understanding these factors is crucial to designing appropriate services for women and for implementing IPV reduction interventions in urban areas. Strengthening formal and informal mechanisms for help-seeking, utilizing multi-modal interventions that address economic stress and challenge inequitable gender norms, as well as tailoring programs specifically for IDPs, are some considerations for IPV program planning focused on conflict-affected women in urban areas.

Spectral Resolution and Coverage Impact on Advanced Sounder Information Content

NASA Technical Reports Server (NTRS)

Larar, Allen M.; Liu, Xu; Zhou, Daniel K.; Smith, William L.

2010-01-01

Advanced satellite sensors are tasked with improving global measurements of the Earth s atmosphere, clouds, and surface to enable enhancements in weather prediction, climate monitoring capability, and environmental change detection. Achieving such measurement improvements requires instrument system advancements. This presentation focuses on the impact of spectral resolution and coverage changes on remote sensing system information content, with a specific emphasis on thermodynamic state and trace species variables obtainable from advanced atmospheric sounders such as the Infrared Atmospheric Sounding Interferometer (IASI) and Cross-track Infrared Sounder (CrIS) systems on the MetOp and NPP/NPOESS series of satellites. Key words: remote sensing, advanced sounders, information content, IASI, CrIS

Possible Gems and Ultra-Fine Grained Polyphase Units in Comet Wild 2.

NASA Technical Reports Server (NTRS)

Gainsforth, Z.; Butterworth, A. L.; Jilly-Rehak, C. E.; Westphal, A. J.; Brownlee, D. E.; Joswiak, D.; Ogliore, R. C.; Zolensky, M. E.; Bechtel, H. A.; Ebel, D. S.;

Coordinated STEM/FIB/NanoSIMS Analyses of Presolar Silicates in Comet Dust and Primitive Meteorites

NASA Technical Reports Server (NTRS)

Keller, Lindsay; Nguyen, A.; Rahman, Z.; Messenger, S.

2012-01-01

Silicate grains were among the most abundant mineralogical building blocks of our Solar System. These grains were the detritus from earlier generations of stars that have been recycled in the early solar nebula. Rare sub-micrometer survivors of this processing have been identified in meteorites, micrometeorites and interplanetary dust particles (IDPs). These silicate grains are recognized as presolar in origin because of their extremely anomalous isotopic compositions that reflect nucleosynthetic processes in their stellar sources (evolved stars, novae and supernovae). We perform coordinated chemical, mineralogical and isotopic studies of these grains to determine their origins and histories. We examine the complex mineralogy and petrography of presolar silicates using imaging, diffraction and chemical data obtained from thin sections with the JSC JEOL 2500 field-emission STEM equipped with a Noran thin window energy dispersive x-ray (EDX) spectrometer and a Gatan Tridiem GIF. Quantitative element x-ray maps (spectrum images) are acquired by rastering a 4 nm incident probe whose dwell time is minimized to avoid beam damage and element diffusion during mapping. Successive image layers are acquired and combined in order to achieve approx 1% counting statistics for major elements. The IDP samples are prepared by ultramicrotomy of particles embedded in epoxy or elemental sulfur. After EDX mapping, the sections are subjected to C, N, and O isotopic imaging with the JSC NanoSIMS 50L ion microprobe. We prepare sections of some meteorite grains using the JSC FEI Quanta 3D focused ion beam (FIB) instrument. The specimen surface is protected from the FIB milling process by layers of electron beam-deposited C and Pt followed by an ion-deposited Pt layer. We also use the FIB to preferentially remove surrounding grains to reduce the background in subsequent NanoSIMS measurements. For mineralogical studies, we again employ the FIB instrument to deposit a protective cap over the

78 FR 36223 - Extension and Redesignation of Syria for Temporary Protected Status

Federal Register 2010, 2011, 2012, 2013, 2014

2013-06-17

... toll-free number). Note: The phone number provided here is solely for questions regarding this TPS... Authorization Document FSA--Free Syrian Army Government--U.S. Government IDP--Internally Displaced Persons IJ.... In addition to the Free Syrian Army (FSA) and Syrian National Army, reports published in 2012...

Site-Specific Three-Color Labeling of α-Synuclein via Conjugation to Uniquely Reactive Cysteines during Assembly by Native Chemical Ligation.

PubMed

Lee, Taehyung C; Moran, Crystal R; Cistrone, Philip A; Dawson, Philip E; Deniz, Ashok A

2018-04-12

Single-molecule fluorescence is widely used to study conformational complexity in proteins, and has proven especially valuable with intrinsically disordered proteins (IDPs). Protein studies using dual-color single-molecule Förster resonance energy transfer (smFRET) are now quite common, but many could benefit from simultaneous measurement of multiple distances through multi-color labeling. Such studies, however, have suffered from limitations in site-specific incorporation of more than two dyes per polypeptide. Here we present a fully site-specific three-color labeling scheme for α-synuclein, an IDP with important putative functions and links to Parkinson disease. The convergent synthesis combines native chemical ligation with regiospecific cysteine protection of expressed protein fragments to permit highly controlled labeling via standard cysteine-maleimide chemistry, enabling more global smFRET studies. Furthermore, this modular approach is generally compatible with recombinant proteins and expandable to accommodate even more complex experiments, such as by labeling with additional colors. Copyright © 2018 Elsevier Ltd. All rights reserved.

Carriers of the astronomical 2175 ? extinction feature

DOE Office of Scientific and Technical Information (OSTI.GOV)

Bradley, J; Dai, Z; Ernie, R

2004-07-20

The 2175 {angstrom} extinction feature is by far the strongest spectral signature of interstellar dust observed by astronomers. Forty years after its discovery the origin of the feature and the nature of the carrier remain controversial. The feature is enigmatic because although its central wavelength is almost invariant its bandwidth varies strongly from one sightline to another, suggesting multiple carriers or a single carrier with variable properties. Using a monochromated transmission electron microscope and valence electron energy-loss spectroscopy we have detected a 5.7 eV (2175 {angstrom}) feature in submicrometer-sized interstellar grains within interplanetary dust particles (IDPs) collected in the stratosphere.more » The carriers are organic carbon and amorphous silicates that are abundant and closely associated with one another both in IDPs and in the interstellar medium. Multiple carriers rather than a single carrier may explain the invariant central wavelength and variable bandwidth of the astronomical 2175 {angstrom} feature.« less

Setting up an early warning system for epidemic-prone diseases in Darfur: a participative approach.

PubMed

Pinto, Augusto; Saeed, Mubarak; El Sakka, Hammam; Rashford, Adrienne; Colombo, Alessandro; Valenciano, Marta; Sabatinelli, Guido

2005-12-01

In April-May 2004, the World Health Organization (WHO) implemented, with local authorities, United Nations (UN) agencies and non-governmental organisations (NGOs), an early warning system (EWS) in Darfur, West Sudan, for internally displaced persons (IDPs). The number of consultations and deaths per week for 12 health events is recorded for two age groups (less than five years and five years and above). Thresholds are used to detect potential outbreaks. Ten weeks after the introduction of the system, NGOs were covering 54 camps, and 924,281 people (IDPs and the host population). Of these 54 camps, 41 (76%) were reporting regularly under the EWS. Between 22 May and 30 July, 179,795 consultations were reported: 18.7% for acute respiratory infections; 15% for malaria; 8.4% for bloody diarrhoea; and 1% for severe acute malnutrition. The EWS is useful for detecting outbreaks and monitoring the number of consultations required to trigger actions, but not for estimating mortality.

Folding propensity of intrinsically disordered proteins by osmotic stress

DOE PAGES

Mansouri, Amanda L.; Grese, Laura N.; Rowe, Erica L.; ...

2016-10-11

Proteins imparted with intrinsic disorder conduct a range of essential cellular functions. To better understand the folding and hydration properties of intrinsically disordered proteins (IDPs), we used osmotic stress to induce conformational changes in nuclear co-activator binding domain (NCBD) and activator for thyroid hormone and retinoid receptor (ACTR). Osmotic stress was applied by the addition of small and polymeric osmolytes, where we discovered that water contributions to NCBD folding always exceeded those for ACTR. Both NCBD and ACTR were found to gain a-helical structure with increasing osmotic stress, consistent with their folding upon NCBD/ACTR complex formation. Using small-angle neutron scatteringmore » (SANS), we further characterized NCBD structural changes with the osmolyte ethylene glycol. Here a large reduction in overall size initially occurred before substantial secondary structural change. In conclusion, by focusing on folding propensity, and linked hydration changes, we uncover new insights that may be important for how IDP folding contributes to binding.« less

Dust Infall Onto Phobos and Deimos Can Explain Their Carbonaceous Reflectance Signature, Perhaps Overlying a Mars-Impact-Origin Core: A Hypothesis

NASA Technical Reports Server (NTRS)

Fries, M.; Cintala, M.; Steele, A.; Welzenbach, L. C.

2017-01-01

Discussions of Phobos' and Deimos' (henceforth P&D) origin(s) include an unresolved conflict: dynamical studies which favor coalescence of the moons from a large impact on Mars [1,2], versus reflectance spectroscopy of the moons showing a carbonaceous composition that is not consistent with martian surface materials [3-5]. One way to reconcile this discrepancy is to consider the combined options of a Mars impact origin for Phobos and Deimos, followed by deposition of carbon-rich materials by interplanetary dust particle (IDP) infall. This is significant because, unlike asteroidal bodies, P&D experience a high IDP flux due to their location in Mars' gravity well. We present some relatively simple, initial calculations which indicate that accreted carbon may be sufficient to produce a surface with sufficient added carbon to account for P&D's reflectance spectra. If this is true, then a major objection to an impact origin for P&D is resolved.